LOCUS       DASULZ010000001         2764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_913366, whole genome shotgun sequence.
ACCESSION   DASULZ010000001 DASULZ010000000
VERSION     DASULZ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913366"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VFY96_00005"
     CDS             <1..765
                     /locus_tag="VFY96_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5604861.1"
                     /translation="DVSYTADGKFILESINWRVAEGEHWAILGPNGSGKTTLLKLMGG
                     YLWPNAGGIIFRKGKRLSYLPGLRKSIGWVTAELAAEIPAHEKALDTVVSGKFAQIGY
                     LGGVWGEASKRDYQVARRYLDQLGFGRLSEQPFGTLSQGEQQKVLIARARMAKPYLII
                     LDEPCAGMDPGARENFLASLRELGKQKRIPALIYVTHHVEEILPIFRHTLILRQGKIL
                     YVGRTAGILRRYILKDLYGVSLTLMKRKGRYWPVMN"
     gene            900..2309
                     /locus_tag="VFY96_00010"
     CDS             900..2309
                     /locus_tag="VFY96_00010"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5604862.1"
                     /translation="MASLATLIVTADSGQLSIAMPLIIKDLHADLSLASWIALVYALV
                     TASLYLPCGRLSDVLGVGKLFLAGFVLYSLSSFAAGMAEAAVQMIFFRAVQAAGSALI
                     MANNFALITALFPREERGRAMGIAGGTVSALGYTLGPVLGGLLTHSFGWRANFYLSGL
                     LAIIGFAAARKLLPAESFKPSGEKKTPFDVIGAITFGAGISLLLFSLALTQKGSWRGP
                     AVGLTALSAVAALGLFIRWEKRASFPLLDLNIFRVAAFTLGNIARGFSFITMSVSNLL
                     MPFFLQLALGLDPLRAGLLVAPTPFAMALLAPITGWLSERFSPERLCAVGLTVNGAAL
                     VLLALLQTDATGFQVAAGLALLGVGMGTFQTPNNNLLMSSVPRHRLGIGSSVLSIVRS
                     LGYSVGATLAAMIVGHFLLSATGEMSLQNLSAAAGISRDTPTLAAFLRGYRWAYLTAA
                     VIAFAGAAVSLWAAADNKN"
     gene            complement(2317..>2764)
                     /locus_tag="VFY96_00015"
     CDS             complement(2317..>2764)
                     /locus_tag="VFY96_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5604863.1"
                     /translation="PVPAREIVKLPLYTAAAAAWLYGIWFCLRLKKVQASAPCQISIA
                     LTVGVILASLTLPHLLYYDLCVLLPAGVLLLAKNSPLQIPGTHSVGVMGWIAITLFFP
                     LLLGFAYTTILPLILELIILLLFVSLLTRLTEQVNAGHALNAESTA"
BASE COUNT          585 a          729 c          795 g          655 t
ORIGIN      
        1 gatgtttcgt atacagcaga tggaaaattc attctcgaat cgatcaattg gagggttgct
       61 gagggcgagc attgggcgat tctcggaccc aacggttcgg gaaagacgac gttgctcaag
      121 ttgatgggcg gatacttatg gccaaacgcg ggtggcatca tttttcgcaa aggaaaaagg
      181 ctttcctatt tacccgggct cagaaaaagt attggttggg taaccgcaga gctggccgct
      241 gaaattcccg ctcatgaaaa agcgctcgat actgtcgtgt ccggtaaatt tgcccagatc
      301 ggctacctcg gcggtgtatg gggtgaggcc agtaagcggg actatcaagt agcccgccgt
      361 tacctcgacc agcttggctt cggtcggttg agcgagcagc catttggcac actttcgcaa
      421 ggagagcagc agaaggtttt gatcgcgcga gcgcgcatgg caaaaccgta cctgatcatc
      481 ctcgatgagc cttgcgccgg aatggatccg ggtgcgcgcg aaaatttttt ggcttcactt
      541 cgagagctcg ggaagcaaaa aagaatcccc gcgttgattt acgtgacgca ccatgtcgaa
      601 gagattctgc caatcttccg acacacgttg attctgcgcc aaggaaaaat cctttacgtc
      661 ggacgcactg ccggtatcct gcggcgctat atcttgaaag atctttacgg cgtttctctc
      721 acgctgatga aacgcaaggg ccgatattgg cccgtgatga actgaacagt tgaagcttca
      781 aacatatcga taagcccccg cttcgcactc ttgaattctc atagagccgc ttcaggttga
      841 tgatccaccc acgagaggaa cccttatcgc cgccccgaag ccgctggtgg tctctcgcgg
      901 tggcatccct agccaccttg atcgtcactg ccgattcagg gcagctcagc atcgccatgc
      961 ccttgatcat caaggacttg cacgctgatt tatccttggc gagttggata gcactggttt
     1021 acgccctcgt cacagcatcg ctctatctgc cttgtggcag actgtcggat gtcctcgggg
     1081 ttggaaaact gtttctcgcc ggctttgttt tgtattcgct gagctccttt gctgccggca
     1141 tggcagaagc agcggtccaa atgattttct ttcgtgcggt gcaagcggcg ggaagcgcac
     1201 tcatcatggc caataacttc gcgttgatca ccgcgctctt tccgcgcgag gaacgggggc
     1261 gcgctatggg catcgccggc gggactgtct cggcactcgg ttacacgctt ggtccggtac
     1321 tcggagggct tctcactcat agcttcggtt ggcgggcaaa tttctatctg tccggattgc
     1381 tcgcaatcat cggatttgcc gccgcccgaa agcttttgcc ggcggagagc ttcaaaccca
     1441 gcggagagaa aaaaacaccc ttcgatgtca tcggggcgat caccttcggt gctggaatct
     1501 cgttgctctt gttcagtttg gcgctcacac agaaaggcag ctggcgagga cctgcggtag
     1561 ggttaaccgc actgagtgcc gtcgccgcac tgggtctgtt tattcggtgg gaaaagcgcg
     1621 caagctttcc cttgctcgac ttgaatattt tccgcgttgc cgcgttcaca ctgggtaata
     1681 tcgccagagg gttcagtttc attaccatga gcgtcagcaa tttattgatg ccgttttttt
     1741 tgcagctcgc gctcggtctg gatccgctgc gcgccgggct tttggtggcg cccacgccat
     1801 ttgcgatggc actgctggcg ccaattaccg ggtggctatc agagcgcttt tcgcccgaac
     1861 ggctctgcgc cgtcggactt actgtgaacg gagccgcgct ggtgcttctg gctctgttgc
     1921 aaacggatgc gactggtttt caagttgccg cgggtctggc tttgctgggt gtcggcatgg
     1981 gaacttttca aacaccgaac aacaatctac tgatgagctc ggtgccgcgc catcggctgg
     2041 gaatcggatc ttctgtgttg tcgattgtcc gcagcctggg ttattccgtc ggcgcaacac
     2101 tggcggcgat gattgtcggc cattttttgc tcagcgcgac aggtgaaatg tcgctgcaga
     2161 acctcagcgc ggccgcagga atttctcggg atacgccgac cctggcggcg tttttacgcg
     2221 gttatcgttg ggcctatctc acggctgcgg tcatcgcctt cgccggcgcc gccgtgagct
     2281 tatgggcggc ggccgataac aaaaactgac gaattctcag gccgtggatt cagcgttgag
     2341 ggcatgtcct gcgttcactt gttctgtaag gcgcgtcagc aaacttacga acaacagcaa
     2401 aatgatcaat tcgagtatca gaggaagtat tgttgtgtaa gcaaagccga ggagcagcgg
     2461 aaaaaagaga gtgatggcga tccaacccat taccccgact gagtgagttc cgggaatttg
     2521 aaggggagaa tttttcgcga gaagaagcac tccggccggc aggagaacgc aaagatcgta
     2581 gtacaataaa tggggtaacg tcaaagacgc caggatgacg ccaaccgtaa gcgcgatcga
     2641 aatttggcag ggcgcactcg cctgaacttt ttttagccgg aggcaaaacc aaataccgta
     2701 cagccatgcc gcggccgcag cggtgtagag cggtaacttc acaatttccc gggcaggaac
     2761 agga
//
LOCUS       DASULZ010000002         4011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1217912, whole genome shotgun sequence.
ACCESSION   DASULZ010000002 DASULZ010000000
VERSION     DASULZ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217912"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            371..1498
                     /locus_tag="VFY96_00020"
     CDS             371..1498
                     /locus_tag="VFY96_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX5604864.1"
                     /translation="MSRIKGGSGKGSSLGAWDIAATLGLVAVAFIVERTAPVDNTKPA
                     LPPKEGRNASTAGLASEDGNRGRDAASPSDIPAKGWKDILWRVYGNVSDHRILALAAG
                     MTYYSILAIFPALAALVAIYGLFTDPASIAKHLDELSGFIPGGAAEVAREQLTRVASK
                     GGSTLGFTFGIGLIISLWSANAAMKALFDTLNIVYGEQEKRGFLKLNAMSLGFTLAGI
                     AFILSALAAVVVIPIILEYLGLSKAADLLIRIARWPAMFLALAIGLSFIYRFGPSRAA
                     PRWRWITWGSAAATIMWIVTSALFSFYAANFGTFNETYGSLGAAIGLMTWLWISAIAI
                     LLGAELNAEMEHQTKRDTTTGGPKPLGTRGAKMADTLGAAR"
     gene            1870..2370
                     /locus_tag="VFY96_00025"
     CDS             1870..2370
                     /locus_tag="VFY96_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HEX5604865.1"
                     /translation="MAKEPKTLDDLFHDTLKDIYFAEKKILATLPKMAKAAQSEELKA
                     AFEKHRTETEGQIERLEKVFAVIEQKPQGKTCAAINGITEEGAEIMEEYKGSPALDAG
                     LLAAAQAVEHYEISRYGTLIAWAEELGLDDAVSLLEETLEEEEATDEALTEIAKTAIN
                     QEAQAA"
     gene            2771..3820
                     /locus_tag="VFY96_00030"
     CDS             2771..3820
                     /locus_tag="VFY96_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5604866.1"
                     /translation="MGAGARMNARVVLALVLFGLAVWTAAGFLPALIWATILAVALWP
                     LYLKFAARFSSGPSSLAAFIFTAIVALVLFTPMSLAVYQIAQQGDVLVSWLKQARESG
                     VEVPDWVARLPVAAESLQSWWRANLSDPKAATALLQTVNADNASEFFKTFGGQLLHRM
                     FLLLFSLLTLFFLLRNGRAVASRFLETCDRLIGDAGEGLVEKMVDATRGTVNGTVLVA
                     VGEGLLIGVGFLVAGVPNPVMFTILTTAFAMLPFGAWLAFTTAALVLVSGGGSGFAAA
                     AVFCWGAIVMLAGDHFVWPTLVGGSARLPFLLAFVGIFGGLATFGLFGLFLGPVIMAA
                     LLTVWREWIYRPVPQ"
BASE COUNT          794 a         1160 c         1221 g          836 t
ORIGIN      
        1 gatggctgcg atgcgcctgg tggaaatcgt tttgagccgc ccatccgcca acggtgccca
       61 ggcggtggaa caggatagga gcgacgaggc gccaagaaag tgcatcgctt tgccgttgct
      121 gacaatctgc agcaactttt gctctgcctc gaaagcggcg agcctgtgac gcagagacaa
      181 accaacatgg acaacctgat cgaattcccg tccaagcgga gggcgagcta aatcaccgcc
      241 atagcggacg gactgcgttc gcgggtcgct tagtctcgtt ggtgtttgca cgctcgtccg
      301 gcgcgagcag atacaccaga gaaagaggaa cagatgagcg tcgaacgcga ttaccttcgc
      361 ggagatgccg atgagcagga taaaaggcgg cagtggaaaa ggctcctcgc ttggcgcctg
      421 ggatatcgcg gcgacgctcg gactcgtggc ggttgccttt atagtcgagc gcacggcccc
      481 cgtggataac acgaaaccgg cccttccgcc gaaggagggc cgcaacgcct caaccgccgg
      541 cctcgccagc gaagacggga accgcgggcg cgacgcggcg tcaccttcag atatcccggc
      601 aaaggggtgg aaagacattc tttggcgcgt atacggcaat gtaagcgacc accgcattct
      661 ggccctggcc gcaggtatga cctactacag catccttgca atttttcccg cgctcgcggc
      721 tcttgtggcc atctatgggc tgtttaccga ccccgccagc atcgcaaagc atcttgacga
      781 actatcgggc ttcataccgg gcggcgcggc agaagttgcg cgggagcagc tgacgcgcgt
      841 ggcatcgaag ggaggcagca cgctcggctt caccttcggg ataggtctca ttatttcgct
      901 atggagcgcc aacgctgcaa tgaaggcgct cttcgacacg ctcaacattg tgtacgggga
      961 gcaggaaaag cgcggcttcc tcaaactcaa cgcgatgtca ctagggttca cccttgccgg
     1021 gatcgcattc atcctgtcgg cgctggccgc ggtcgtcgtc attcccatca tcctggaata
     1081 tctcggactc tcgaaagccg ccgatctcct gatccggatc gcgcgctggc cggcaatgtt
     1141 tcttgcgctc gcgatcggcc tttccttcat ctaccgcttc ggaccgagcc gcgcagcgcc
     1201 gcgctggaga tggatcacat ggggcagcgc agccgcgacg atcatgtgga ttgtgacctc
     1261 tgcgcttttt tccttttacg cggcgaactt cgggacgttt aacgaaacct atggctcgct
     1321 gggcgcggcc atcggtttga tgacgtggct ctggatttcg gcgatagcca tactgcttgg
     1381 cgctgaactc aacgcggaga tggagcacca aactaagcgc gacaccacta ccggcgggcc
     1441 aaagccgtta ggcacgcgag gtgcaaaaat ggctgacaca ctgggagcgg ctcggtagtg
     1501 agcaatccgc tacgaaattc cggcgatttg aaaccttgcc gaggtgcgtg aggccccttc
     1561 catcatttaa acgccctccg ctcccgcgcc gcctaggcgg ctccactctg cgtggtagct
     1621 gcggcagcgg ctgggcttgc cggtaaagcc gagggagggt tcacccttga agcatgcgcg
     1681 ctccggcgca gatggcatcc tgaagagacc tgcttcgggg cgggtttcag tgaagaaggg
     1741 aaataacggg cccgatccaa ctaaaaagtc gcaggacgct ttaaacagga acctgtgacc
     1801 gaaaggcccg ttgggggcgg taagctcacc acgcggtgag acaccaattc accctaaagg
     1861 actccaatca tggccaagga accgaagaca ctcgacgacc tgtttcacga cacgctgaag
     1921 gacatctact tcgcggagaa gaagatcctg gcaaccctgc cgaagatggc aaaggctgct
     1981 cagagcgagg agctcaaggc ggcattcgag aagcatcgca ccgaaacgga aggtcagatc
     2041 gagcgcctcg aaaaggtgtt cgccgtcatc gaacagaaac cgcagggcaa gacctgcgct
     2101 gctatcaacg gtattacgga agagggtgcc gagatcatgg aagaatataa gggctctccg
     2161 gcattggacg ccggcctcct tgccgccgcg caggcagtcg agcactacga gatttcccgc
     2221 tacggcacgc tgatcgcgtg ggccgaagag ctcggcctcg atgacgcggt ctcgctcctt
     2281 gaagaaaccc ttgaggagga agaggcaact gacgaagcac tgacagagat tgcaaagact
     2341 gcgatcaatc aggaagctca agcagcctaa ctagcaacag aaaatgcccc gcattgcggg
     2401 gcattttttg accgggacca ctctgtacgc tcaactttaa ggctgaggcg ccattcccca
     2461 ccgcgcacgg ggttgccttc atagtttcgg tagtcgaggc tgacgccgtc gacgattgag
     2521 atcacgaggg acctaaaggg aaataacttc tcgcgaataa actaattaga ccagtttccc
     2581 gtagcgcgcc cgtctttcgt aggattgcca cgttttccca gcggggaccg aacgaccgtc
     2641 ctgtagttct gtcatgacaa taggggcgcg acaagcgatg gcaagaaggg tgattttagc
     2701 cgcgtccaaa aaacggccgt ccgagcggat cgacgaagcg cctgccagga cggagaggaa
     2761 gcctcttcct ttgggcgccg gcgcgcgcat gaatgcccgg gtcgtgcttg ccctggtcct
     2821 gtttggtctt gcggtgtgga ccgccgcggg ttttctgccg gcgctgatct gggccacgat
     2881 tctcgcggtt gccctgtggc cgctgtatct caaatttgcg gcccgctttt cctctggccc
     2941 ctccagcttg gctgcgttca tctttaccgc aatcgttgcg ctggtcttgt tcactccgat
     3001 gtcgcttgcc gtctaccaga tcgcgcagca aggcgacgtg ctcgtgagtt ggctgaagca
     3061 ggcccgcgag agtggggttg aagtcccgga ttgggtcgct cgtctgccgg ttgctgcaga
     3121 aagtctgcaa tcatggtggc gcgccaacct ttcggacccg aaagctgcca ccgccctgct
     3181 tcaaactgtc aatgctgaca atgcgtcgga gtttttcaaa acgtttggcg gacagctgct
     3241 ccatcggatg ttcctgttgc tcttctcgct cctgactttg ttctttctgc tccgtaatgg
     3301 tcgcgccgtc gcgagccgct tcctcgagac gtgtgaccgc cttattggcg atgccgggga
     3361 ggggctggtc gagaaaatgg tcgatgccac gcgcggcacg gtgaacggca cagtgctggt
     3421 cgccgtgggc gagggattgc tgatcggcgt tggtttcctc gtggcgggtg taccgaatcc
     3481 ggtgatgttt acgattctaa cgaccgcttt cgcgatgctg ccgtttggtg catggctcgc
     3541 cttcaccacg gcggcgttgg tcctggtctc cggcggcggc agcgggttcg cggccgcagc
     3601 cgtattttgc tggggcgcga tcgtcatgct tgccggcgat catttcgtct ggccaaccct
     3661 ggtcggcggc tcggcgcgtc tcccgtttct tctcgccttt gtcggaatct tcggaggcct
     3721 cgctaccttc gggttgttcg gattgtttct ggggcctgtg atcatggcgg cgttgctgac
     3781 cgtttggcgc gagtggattt accggcccgt tccacaatag aattctgcag cggcgcctat
     3841 gatcacggct ctgagcgcgg tcggtattcg atatcgattt ggcgagcaac cctgcttcgc
     3901 agccgtcgcc ttaaaaaatt accgaatatt cccgaagttc ggaaccggca cgcatgtcgc
     3961 gggtttcagg agctcgcttc aatttgttgg aggacaacat gaagaatctt g
//
LOCUS       DASULZ010000003         5299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_273, whole genome shotgun sequence.
ACCESSION   DASULZ010000003 DASULZ010000000
VERSION     DASULZ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5299
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VFY96_00035"
     CDS             complement(<1..617)
                     /locus_tag="VFY96_00035"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HEX5604867.1"
                     /translation="MAIITIYQGASGSGEELAGALAQSLGCRCISREVLVEASLRYGI
                     PEAKLSKIVEREPNWWSRFMENLQPYRIALQAAFCQIAVTEGQENLVYHGHLGHELLP
                     DYKHVLKVLLTAPMARRVEQVKARQGLSDTAARKFIDETDKARSRRLMGMFGVDWRDA
                     SRFDLVVNLGRMSINTIKPLIVETLSQPDYEITPGSKQAFEDFALA"
     gene            975..2156
                     /locus_tag="VFY96_00040"
     CDS             975..2156
                     /locus_tag="VFY96_00040"
                     /inference="COORDINATES: protein motif:HMM:NF014510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HEX5604868.1"
                     /translation="MFTPFFNIGVADVIDILVVTVLLYTATAWLRQTRAAFVIRGIFI
                     LAAVYIVARYLDLQLTAWLFQGFFAIFLIIIVVIFQEELRQIFERIAVWSLTTKPARP
                     LHSDAVDILVGTLADLAKEKIGALVVVEGRDPIDRHIMGGIELGGKISEPLLKSIFDP
                     HSPGHDGAAIIENDRISRFAAHLPLSNNFQQLSNVGTRHSAALGLTELSDALCIAVSE
                     ERGTISFARNGRLRQVDNIQELGLLLRRFLQEKYPSSERRTFSLALVRENWVVKLVTF
                     SLAVGLWYVLIPGSSTVEVTYKLPVQVQNLPADYHVEEVMPSQVNATFTGPRRAFYFF
                     DPGKLKVTVDLSATDTGRKVLRLTEQNVQHPQDLTLQQLDPQTLRVSVRKVSREQTDK
                     G"
     gene            complement(2207..>5299)
                     /gene="treS"
                     /locus_tag="VFY96_00045"
     CDS             complement(2207..>5299)
                     /gene="treS"
                     /locus_tag="VFY96_00045"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEX5604869.1"
                     /translation="YGTLKDFKIFLSEAHQRGIRVITELVLNHTSDQHPWFQRARRAP
                     AGSKERDYYVWSDNQEQYKEARIIFKDFETSNWAWDPVAKGYFWHRFYSHQPDLNFDN
                     PAVKTAIFEAVDFWLDMGVDGLRLDAVPYLFEREGTSCENLPETHQFLKELRRHVDEK
                     YRNRMLLAEANQWPEEAVTYFGDGDECHMAFHFPVMPRIFMSVHMEDRFPIIDILNQT
                     PEIAANCQWTLFLRNHDELTLEMVTDEDRDYMYKVYASDPKARLNLGIRRRLTPLLGG
                     NRRKIELLNALLFSLPGTPVIYYGDEIGMGDNIYLGDRNGVRTPMQWSADRNAGFSRA
                     NPQRLYLPIIIDPEHHYEAINVEAQQQNLSSPLWWMKRLILLRKRFNAFSRGSTEFLF
                     PENRKVLVFIRRLQDEIILTVANLSRFVQCVEIDLSPFKGSVPVELFGQTEFPPIGDL
                     PYFVTLGPHSFYWFKIETTPAAAARSSEEAPLASLELAGGWEQILKGSGKSALEKILP
                     AYLKSCRWFGGKARRIRAVNLSESVSVPLDSDGALFTVVEVNYREGEPDYYFLPLTFV
                     SGDRVTELTQSSPQAVVARLKVRRSGGSVDGVLLDALYDRIFLKALLEAIFRRRTFQG
                     KSSEISAVPSKLLRKLSGAEKDLPDPIPAKREQSNTSVVYGDRFILKMFRRLQEGVNP
                     ELEIGSFITERTSFEHVPKLAGSFQWSRGSRSATLGVLQSWIRNEGDAWRYTLDHLGD
                     FFEHGSTVGETPASFSPPARHVLDFVAHELPLGPRELIGSYLASARLLGQRTGELHVV
                     LASDRNDSDFAPEPFTPFYRRSVYQSMRTAADRTLTLLRERTKNLPDEIRGEAEKILT
                     FRSEIFGRYRFVVDQKITALRIRCHGDYHLGQVLFTGKDFVITDFEGEPTLPLGERRA
                     KRSPLRDVAGMLRSFEYAALSGLRSGDFRPEQLPILKPLARAWVFWVSVAFLESYLEA
                     SRSGGFLPNDVEELKNLLDLYLLEKAVYELRYELNNRPDWVAVPIQGISELLHSRGRD
                     "
BASE COUNT         1177 a         1531 c         1430 g         1161 t
ORIGIN      
        1 gccaaagcaa aatcttcaaa cgcctgtttc gaaccgggcg tgatctcata atccggctgg
       61 cttagggttt ccacaatcag aggtttaatg gtgttaatgc tcatgcgccc gaggttcacc
      121 acgagatcga accggctcgc atcgcgccag tcgacaccga acatccccat gagccggcgg
      181 ctgcgcgctt tgtcggtttc gtcgataaat tttcgcgccg ccgtgtcgct cagcccctga
      241 cgggctttga cctgctcgac ccttcttgcc atgggagcgg tcagcaaaac cttcagcacg
      301 tgtttataat cgggcaaaag ctcgtgtccc aaatgaccat ggtaaacgag gttctcttgc
      361 ccctcggtaa cggcgatttg gcaaaaggca gcctgcagag cgatgcggta aggctggaga
      421 ttctccataa accggctcca ccagtttggt tctcgctcga cgattttgct aagcttcgct
      481 tccggaatac cgtatcgcaa gctcgcttcc accagcacct cccgactgat gcaacggcaa
      541 ccaagcgatt gcgccaatgc gccggcgagt tcttcaccgc tacccgatgc gccttgatag
      601 atggtgatga tggccatgtt tacctcctca tcgagtcaga taatatcccc ttcggtcgat
      661 agaaccgccg ctgcaggtgc gcggcacagc ctaggacatc gctgtgctgg gcaagacatc
      721 ggcgcaattg acgatagaaa aaccgcagga cttcaggtgc cggaccgaac atcggatgga
      781 agctcctcct tccccggagc acgacctcgg ttccgctgag cagtgtggct tcgcaggtta
      841 ccggggccac gctagcaccc gcatcggaca cggtcaaacc gcttttacaa ctcgcttctt
      901 cccgttgcca tcggcactcg attgccagtc agcggtgctt cgtgatttaa tgcaccagcg
      961 agagaaaatc tgccatgttc acgccatttt ttaacatcgg cgttgccgat gtaatcgata
     1021 ttctcgtcgt cacggtattg ctttacacgg cgaccgcatg gctacggcaa acccgagcgg
     1081 cctttgtcat ccgcggaatt tttatccttg cggccgttta tattgtcgcc cgctacctcg
     1141 atcttcaatt gaccgcatgg ctctttcaag gtttcttcgc gatttttttg atcatcatcg
     1201 tcgtgatctt tcaggaagag ctgcgacaga ttttcgagcg tattgcggtc tggagtctaa
     1261 cgacgaagcc agcccggcca ctgcactcgg acgctgtgga tattctggtc ggcacccttg
     1321 ccgatctggc caaggagaaa atcggcgcgc tggtggtcgt ggaagggcgc gaccccatcg
     1381 atcgccacat catgggcgga atcgaattgg gcggcaaaat tagcgagccg ttgttgaaaa
     1441 gtatctttga tccacattct cccggccacg acggcgcagc gatcatcgaa aacgaccgga
     1501 tctcgcgctt cgcggctcac ttgccgctct ccaataactt ccaacaacta agcaacgtcg
     1561 gcacgcgcca cagcgcggct ctcggcctga ccgagctgag cgacgccctg tgcattgccg
     1621 tttccgaaga acgcggcacc atctcgttcg cgcgcaatgg ccgccttcgc caagttgaca
     1681 atattcagga actcggcctg ttgctgcgca gatttttaca ggagaaatat ccgtcgtcag
     1741 agcggcgcac gttttcgctt gcgctggtgc gggaaaactg ggtggtcaag cttgttacct
     1801 tttctctggc ggtaggactc tggtatgtcc tgattcccgg ctcgagcacg gtcgaagtta
     1861 cctataaatt gcccgtccaa gttcaaaacc tccccgccga ttatcacgtg gaagaagtca
     1921 tgccgtcaca agtgaatgcc accttcaccg gaccgcgccg agcattttat tttttcgatc
     1981 ccggtaagct caaagtgacg gtcgatttat ccgccaccga caccgggcga aaagtgcttc
     2041 ggctgacgga gcaaaacgtc cagcatcctc aagacctgac gcttcagcag cttgatccgc
     2101 agaccctgcg ggtctcggtg cgtaaggttt cccgcgagca aacagacaaa ggataaacca
     2161 gtcgatactt cggactggag aaaagtagcg agggttatct gccgaatcag tctcggcccc
     2221 gagagtgtaa aagttcgctg atcccctgta tcggcacagc tacccaatcg ggtcgattgt
     2281 tcagttcgta gcgcaattcg tagacggcct tttccagcag atacagatcc aacaggtttt
     2341 tcagttcttc gacatcattg ggtaaaaatc cgccgctacg gctggcttcc aggtagcttt
     2401 cgagaaaagc gaccgacacc caaaagaccc aggcgcgcgc caatggcttg aggatgggaa
     2461 gttgttccgg gcggaaatca ccgcttctta atcccgagag cgccgcatat tcgaaggacc
     2521 tcagcatgcc cgcaacatcg cgcaacggtg atcgcttggc gcgccgctcg cctagaggta
     2581 gcgtcggctc gccctcgaaa tcagtaatga cgaaatcttt gcccgtgaaa agcacctggc
     2641 cgagatgata gtcaccgtga cagcgaattc gtagcgcggt gattttttga tcgacaacaa
     2701 accgataccg gccaaagatc tcgctcctga aggtcagaat cttttcggcc tcaccacgga
     2761 tctcatcggg caagtttttt gtccgctcgc gcaacaaagt gagcgttcgg tccgctgcgg
     2821 tgcgcatgct ctgatagacc gagcggcgat aaaacggcgt aaacggctcg ggcgcgaaat
     2881 ccgaatcgtt acgatcggaa gccagcacga cgtgaagctc accggtgcgt tggcccaaga
     2941 ggcgcgccga tgccagataa gacccgatca gctcacgcgg ccccagcggc aactcatgag
     3001 cgacaaagtc caagacgtgt cgggcagggg gcgaaaaact cgccggagtt tcgcccacgg
     3061 tcgacccatg ttcaaaaaaa tcgccaaggt gatcgagcgt atagcgccag gcatctcctt
     3121 catttctgat ccagctttgc aaaaccccca aggtcgcgct ccggctgccg cggctccact
     3181 gaaagctgcc ggcaagtttg ggaacatgtt cgaacgacgt tctctcggtg atgaaactgc
     3241 cgatctccag ttcggggttc acgccttcct gcaaacgccg aaacattttg aggatgaagc
     3301 gatcgccgta cacgacggaa gtattgcttt gttctcgctt ggccggaatc gggtccggca
     3361 ggtctttttc agctccgctc aattttcgca gtagttttga cggcactgcg gaaatttctg
     3421 acgatttacc ctgaaaagtt cggcgccgaa agatcgcttc gagtagcgcc ttgagaaata
     3481 tgcgatcgta cagggcatcc aggagaacgc cgtcgacact gccgccgcta cgacgcactt
     3541 tgagtcgcgc gaccaccgcc tgaggcgatg attgcgtcaa ttcggtaacc cgatctccgc
     3601 tgacaaacgt gagcggcaag aaataatagt cgggctcacc ctctctgtaa ttgacttcga
     3661 ccaccgtaaa aagcgcgcca tcggaatcga gcggcactga caccgattcg gatagattga
     3721 ccgcgcgaat cctgcgcgcc ttgccgccga accagcgaca actcttgaga taggccggta
     3781 aaatcttttc cagagccgac ttgccgctgc cctttaaaat ctgctcccag ccgccggcga
     3841 gttccaggga agcgagcggc gcttcctcgg agctgcgtgc cgcagcggcc ggagtggtct
     3901 cgatcttgaa ccagtagaag ctgtgcggcc cgagagtaac aaagtacggc aaatcgccga
     3961 tgggcgggaa ttcagtttgt ccgaacagct cgacgggaac cgagcctttg aagggcgaga
     4021 gatcgatctc cacgcactgc acaaagcgtg acagattggc caccgtcaat atgatctcat
     4081 cctgtaggcg gcgaatgaat accaaaacct tgcggttctc aggaaagaga aactcagtgc
     4141 tgccccggct aaaggcatta aagcgcttgc gcagcaagat caatcgcttc atccaccaga
     4201 gcggcgagct taaattctgc tgctgtgcct cgacattgat cgcttcgtaa tgatgctcgg
     4261 ggtcgataat gatgggcaga taaagccgct gcggattggc gcgcgagaac cctgcgtttc
     4321 ggtccgcact ccactgcatc ggcgtgcgca cgccgttccg atcgccgaga tagatattgt
     4381 cgcccatgcc gatctcgtcg ccatagtaga tgaccggcgt ccccggcaaa gaaaaaagca
     4441 atgcgttgag cagttcgatt ttgcgccggt taccgcctaa taacggcgtc agccggcgcc
     4501 ggataccgag attgagccgc gcctttggat cgctcgcata caccttgtac atgtaatcgc
     4561 gatcttcatc ggtgaccatc tcgagggtca gctcgtcgtg attgcgcaga aacaaggtcc
     4621 attggcagtt cgcggcaatt tccggagttt ggttgaggat atcgataatc ggaaaacggt
     4681 cttccatgtg caccgacatg aagatgcgcg gcatcaccgg aaagtgaaac gccatatggc
     4741 actcgtcgcc atcgccgaaa tacgtcacgg cttcttcggg ccactgattg gcctcggcga
     4801 gcagcatgcg attgcgatat ttctcatcga catggcggcg cagctctttc aagaactgat
     4861 gcgtttccgg cagattttcg cagctcgttc cttcgcgctc gaataaatag ggcaccgcat
     4921 cgagtctgag cccgtccact cccatgtcga gccagaagtc aaccgcttcg aagatcgctg
     4981 tcttgaccgc cggattgtca aaattcaggt ccggctggtg tgaatagaac cgatgccaaa
     5041 aatagccttt ggccaccgga tcccatgccc agttggaagt ctcgaagtct ttgaagataa
     5101 tgcgcgcttc tttgtactgc tcctggttgt cactccagac gtagtagtcg cgctctttgc
     5161 ttccggcggg agcgcgccgc gcgcgctgaa accagggatg ctgatccgag gtatgattca
     5221 ggaccagctc ggtaatcacc cggatgccgc gctggtgcgc ttcggagaga aagatcttga
     5281 agtctttgag ggtgccgta
//
LOCUS       DASULZ010000004         5394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_406328, whole genome shotgun sequence.
ACCESSION   DASULZ010000004 DASULZ010000000
VERSION     DASULZ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406328"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..442)
                     /gene="trxA"
                     /locus_tag="VFY96_00050"
     CDS             complement(116..442)
                     /gene="trxA"
                     /locus_tag="VFY96_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX5604870.1"
                     /translation="MGKITEVGDNNFETEVLNSTVPVLVDFWAPWCGPCKSIAPIVEE
                     LAGEYEGKLKVAKLNVDDHPATASRYGVRGIPNLIILKGGAVKDQIVGAVPKAKLVSA
                     IEKALQ"
     gene            complement(454..1932)
                     /locus_tag="VFY96_00055"
     CDS             complement(454..1932)
                     /locus_tag="VFY96_00055"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX5604871.1"
                     /translation="MEVRFRDSRIDRTKADLLVLPVREKKVDAPDVAALDRRLKGHLR
                     AQIERSKFTGAEGSALLYPSAGMLPANQILLIGMGSASESALDSWRTAGARARKESVA
                     QGAEEIAVFFSPERESEAAAAALVEGALLAGYQFNKYRSNSKRSAQPKFLTLFRPGFK
                     TAAPLLRSVGQAQAVSAGVFLARDLVNEPPSIATARFLGEQAEEHCRGRGLSTEVWNK
                     KKIEAMKLAGLLAVNRGSHEEPRFIKIRYQPARRPKKKIALIGKGITFDSGGLSLKPS
                     KSMETMKLDMAGGAAVIATMSCLPKLNLDIEVIGYVPTTDNLPGHNAQKPGDVIRYLN
                     GKTIEVLNTDAEGRLILADALALAAEQKPDSMINLATLTGACVVALGPRVGGLFSNHQ
                     PLADALLRSATDTGEKLWQLPLVKEYKDMIKSSVADIKNIGGAHAGAITAALILQEFV
                     GDLPWAHLDIAGPAFAESENSLGPKGGTGFGVRTLVKFLTSL"
     gene            complement(1943..2494)
                     /gene="folE"
                     /locus_tag="VFY96_00060"
     CDS             complement(1943..2494)
                     /gene="folE"
                     /locus_tag="VFY96_00060"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX5604872.1"
                     /translation="MLEEHIRHILKAIGEDPEREGLLKTPHRVAQALSFLTRGYEMDP
                     AKVINDALFTEEYEEMILQKDIDFFSLCEHHLLPFFGKAHVAYIPHHKIVGISKLARL
                     VDVYARRLQVQERMTNQIATIIMEKLDALGVAVVIEAEHLCMRMRGVEKQNSYVITSS
                     LLGAFRTRQDTRNEFMTLIRSKT"
     gene            complement(2590..3327)
                     /locus_tag="VFY96_00065"
     CDS             complement(2590..3327)
                     /locus_tag="VFY96_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX5604873.1"
                     /translation="MTDVNVFVAFAAGIFSFLSPCVLPLIPSYLSFVSGVSLDEMRNE
                     QSRARVRWRVVLNSLAFILGFSLVFISLGASASFLGELFLAYRNVIRIFGGLFILLVG
                     LYLIGVFKIAALEQYLQFNLKDKPAGYFGSIVVGITFAVAWTPCVGPILGATLALAST
                     AGEVGRGTLLLTSYAAGLALPFFLSALAIDSFFEFSHRLRRYIQPIHTIGGLLLVIVG
                     LLLISDYMTFLNAYALRFTPRWLLDRL"
     gene            complement(3324..3845)
                     /locus_tag="VFY96_00070"
     CDS             complement(3324..3845)
                     /locus_tag="VFY96_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX5604874.1"
                     /translation="MVSMFAPTFTSVWAQTKIDYKKAVPILQPMKEAAPTPDFNLTTP
                     EGKKISLKDFRGKVVLLNFWASWCVPCREEMPAMEKLYQEYKDKNFAVLAVAVKDRKQ
                     DAVDFVKELKITYPIALDPEAQVGSLYGAWGLPATYLIGPKGEGLARGWGPAEWYGNG
                     ARGLIKDLTDGKR"
     gene            complement(4489..>5394)
                     /locus_tag="VFY96_00075"
     CDS             complement(4489..>5394)
                     /locus_tag="VFY96_00075"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX5604875.1"
                     /translation="EEVEKPLGSQLSKLEEQIYSLKRQKLDALINDKLLDKEAAKRKL
                     TVPQLIDAEVTSKVTLVSEQEIEKFYQDNKAQIKTDDPQLCDKIRAYLQNQKLTAKRE
                     EFLKSLRSQAKIVDNLKPPPIARVAVSTEGAPVRGAANAPVTLVMFSDFECPFCKRTH
                     PTINQLLERYAGKIKLVYRDLPLESIHPQARRAAEAARCANAQGKFWEYYDVLFTESP
                     KLGSEDLKRYAAQIGIEPKKFDDCLAAGTYKEIVQKDADEATRLGINGTPAFFINGRP
                     LTGAQPYEMFARLIDEELSQPAAMP"
BASE COUNT         1255 a         1463 c         1421 g         1255 t
ORIGIN      
        1 atcatgaacg tgagcatgcg ccggtacgtt ttctgactaa aaaaaaccgc cgcatcggct
       61 ctcaaaagat ccgacgcggc ggcccgctat tcttacctga gttgtttcaa tctgcctact
      121 gcagtgcttt ctcgattgcg cttacgagtt tcgctttcgg cacggcgccg acgatttggt
      181 ctttgaccgc gccacccttc agaatgatca aattgggaat tccccgcacg ccgtagcgcg
      241 aagcggtggc ggggtgatca tcgacattga gcttggctac tttgagcttg ccctcgtact
      301 caccggcgag ttcttccacg atcggcgcga tcgacttgca tggaccgcac cagggcgccc
      361 aaaaatccac aagcaccggc accgtcgaat taagcacttc ggtctcgaaa ttgttgtcgc
      421 ccacttcggt aattttgccc atagaactcc ctcctaaaga ctggtaagga atttaaccag
      481 agtgcgaact ccgaatccag tgccgccttt cggcccgagc gagttttcgc tttccgcgaa
      541 cgccgggccc gcaatatcga ggtgcgccca gggaagatcg ccgacgaatt cctgcaaaat
      601 caacgctgcc gtgatcgcgc cggcatgtgc accgccgata ttttttatgt cagcgacgct
      661 actcttgatc atatccttgt attctttcac cagcggcagc tgccatagct tctcacccgt
      721 gtccgtggca gagcggagca gcgcgtcggc gagcggctga tgattgctga agagtccgcc
      781 cacacgaggc ccaagcgcca ccacgcaggc gccggtcaag gttgccagat tgatcatgga
      841 atcgggcttc tgttcggcgg cgagggcgag ggcatcggcg aggatcagcc gaccttcggc
      901 atcggtgttc aggacttcaa tggttttgcc gttgagataa cgaatcacat caccaggttt
      961 ttgcgcgttg tgtcccggta aattatccgt ggtcggaacg taaccgatga cctcgatgtc
     1021 gaggttgagc ttcggtaggc aactcatcgt ggcaatcacg gcggcgccgc ccgccatatc
     1081 gagcttcatg gtctccatcg acttggacgg tttcaatgac aatccgccgg aatcgaaggt
     1141 gatgcctttg ccaatcagag cgattttctt tttcggccgg cgcgccggct ggtagcgaat
     1201 ttttatgaac cgtggctctt cgtgactgcc gcgattgacg gccaaaagac cagcgagctt
     1261 catcgcttcg atcttttttt tgttccagac ttcggtcgat aaaccacgcc cgcgacaatg
     1321 ctcttcagcc tgttcgccga gaaatcgcgc cgtggctatc gacggcggct cattgaccaa
     1381 atctcgcgcc aggaaaactc cagcgctgac ggcttgtgct tggccgacgg atctcaacaa
     1441 cggcgctgcg gttttgaagc ccggcctgaa cagggtcaaa aatttcggtt gcgcggatct
     1501 tttggagttg gagcgatact tattgaattg ataaccggca agaagcgcgc cttcgaccag
     1561 tgcggccgcg gccgcctccg actctctctc cggcgaaaaa aaaactgcaa tttcttccgc
     1621 cccctgggcg accgattcct tacgagcccg ggcgccggcc gtgcgccaac tgtccagtgc
     1681 gctttcggat gcgctaccca tcccgattag caggatctga tttgctggga gcatgccggc
     1741 gcttggatag agcagagcgc ttccttccgc gccggtaaat ttgcttctct cgatttgggc
     1801 gcgcaaatgt cctttcaagc ggcggtctaa ggcagctacg tccggcgcgt cgactttttt
     1861 ttcgcgcaca ggcagcacca ggagatctgc cttggtgcga tcgatgcggc tgtcgcgaaa
     1921 tcttacttcc atgcgcactg gttcaggttt ttgagcggat gagagtcatg aactcgttac
     1981 gggtgtcttg gcgggtgcga aatgccccca gcaggctgct ggtgataaca taagaatttt
     2041 gcttctctac accgcgcatg cgcatgcata gatgctccgc ctcgatgacg acggcgacac
     2101 caagggcgtc gagcttttcc ataatgatcg tcgcgatttg attggtcatg cgttcctgca
     2161 cttggagacg gcgcgcgtag acgtcgacca gacgagcgag cttggaaatc ccgacgattt
     2221 tatgatgcgg aatataggcg acatgggctt tgccgaagaa tggcagcaag tggtgttcac
     2281 agagagaaaa gaagtcaata tccttttgca ggatcatttc ttcgtactct tccgtgaaga
     2341 gcgcgtcatt gatcactttt gccggatcca tttcatagcc gcgggtcaaa aaagacaacg
     2401 cctgggccac ccgatgcggt gttttcaaca gtccctcgcg ttcgggatct tcaccaatcg
     2461 cttttagaat atgacgaata tgttcttcca atattgcccc ggcaggatgt gagatccaac
     2521 gtatggtaaa ttctcaaaaa actaacattt agctcatgag cggtcaaatc gagcttcgtc
     2581 gagagagccc tacagacggt cgaggagcca acgcggcgta aaccgcaagg cataggcgtt
     2641 caggaacgtc atgtagtccg agatcaagag cagcccgaca atgaccagaa gcagaccacc
     2701 gatcgtgtgg atgggttgaa tatagcggcg cagccgatgg gaaaactcaa aaaacgaatc
     2761 gatcgcgagg gcgctcaaaa agaaaggcag cgccagtccc gcggcatagc tcgtcaacag
     2821 tagcgtgccg cggccgactt cgcctgcggt gctggcgagc gcgagggttg ctcccaggat
     2881 cggtccgaca cacggggtcc aggccacggc gaacgtgata cccaccacga tcgagccgaa
     2941 gtagccggcc ggcttatctt taagattgaa ttgcaaatat tgctcgaggg cggcaatctt
     3001 aaaaactccg atcaaataaa gtcccacgag caggatgaag aggccgccaa aaattctgat
     3061 tacgtttcgg taagccagaa agagctcgcc gagaaagctc gccgatgcgc caagcgaaat
     3121 gaataccagt gaaaaaccca gaatgaaggc gagggaattc agcacgacgc gccagcgcac
     3181 gcgcgcacgc gattgctcat tgcgcatctc atcgagggaa actcccgaaa cgaatgaaag
     3241 ataggaagga atcagcggca aaacacaggg agagaggaac gagaaaatac ccgcggcgaa
     3301 agcgacaaag acattgacat cggtcatctt ttgccgtcgg tcagatcttt aatcagtccg
     3361 cgcgccccat tgccgtacca ttccgccggt ccccagccac gcgccagtcc ctcgcccttg
     3421 ggaccgatca aataagtcgc cggcaatccc catgcgccat aaagactgcc gacttgcgct
     3481 tcaggatcga gcgcgatggg ataggtgatc ttgagttcct tgacgaaatc gacagcatcc
     3541 tgtttgcggt ctttcactgc cacagccaag acggcaaaat tcttgtcctt atattcctgg
     3601 tacaactttt ccatcgccgg catctcttca cgacacggca cacaccagct tgcccaaaaa
     3661 ttcaacaaca ccaccttgcc acgaaaatcc ttcaaagaaa ttttttttcc ctcgggcgtg
     3721 gttaaattaa aatccggtgt gggcgccgcc tctttcatcg gctgaagaat cggcacggct
     3781 tttttataat cgattttggt ttgagcccaa accgaagtga aagtcggcgc aaacatgcta
     3841 accatgagaa caattgcaaa ctctaatgac cgagggagtc gcgctttcat aaccggacac
     3901 tgtaacgaac gggtgccgtg gacgcaatac acgaacgacg cagaatcagc agggattaaa
     3961 gaggcttcag gaatgcagaa gagacgggca ctaaaaacac aaggccctcc agatcgatgg
     4021 ttggagggct ctgtgtatca ggtaaccaga acggttaccg tattcgcctg agagtaagga
     4081 gggcttaaat cagcaccaca accccaccac tcaacagtta cttctttttc tttttggctt
     4141 tcttcttggc tttcttctta gcagccatag acacctcctt ttagattcca cggggcgggc
     4201 ttatcgacgg agagcataaa gcaacgaact caggcgcaaa gaaaatttct actaaaccct
     4261 cccatatttt gtgggtgatt cgattattaa ggcacaacaa attgtatgtc aagaaaaaaa
     4321 tgcagtttta gaaattattt agatcgaatt gcgaatgcga gtgatgaata tatctcgcga
     4381 tcgcttgctc aattgtgatc gacagtcagc ttacgatcgg cgtcaagagc ttagaagcga
     4441 ttttctgcgg catcgctcaa aggcagtcga agtttggtca ttcggagatt atggcattgc
     4501 ggctggttgt gacagttctt catcgatcag acgcgcgaac atttcgtaag gctgcgctcc
     4561 cgtcaacggc cgcccgttga tgaaaaaagc tggcgtcccg ttgattccca agcgcgtagc
     4621 ttcatctgca tccttctgga cgatttcttt gtacgtgccc gctgcaaggc agtcatcgaa
     4681 ttttttcggc tcgatgccga tttgcgcggc gtagcgcttc agatcttcag aacccagttt
     4741 cggcgattca gtaaacaaaa cgtcataata ttcccagaat tttccctgag cgtttgcaca
     4801 gcgggccgcc tctgcagcgc gccgtgcctg cggatggata ctctcgagtg gtaaatcccg
     4861 gtaaacgagc ttaattttgc cggcatatct ctcaagtaac tggttgatgg tgggatgcgt
     4921 gcgcttacag aacgggcatt cgaaatcgga aaacatcacg agcgtcaccg gcgcattcgc
     4981 cgccccccgc acgggtgcgc cttcggtcga aacagcgacc cgcgcgatgg gcggcggctt
     5041 taaattgtcg acgattttgg cttgcgagcg aagcgattta agaaactcct cgcgttttgc
     5101 ggtaagcttc tggttctgca gataagcacg gattttatca cacagctgcg gatcgtcggt
     5161 ttttatctga gctttgttgt cctggtaaaa tttctcaatc tcctgctcgc tcaccagggt
     5221 gacttttgac gtcacctcgg catcgatcag ttgaggcacc gtgagcttgc gtttggccgc
     5281 ttccttgtcc aataacttgt cattgatcaa ggcgtcgagc ttctggcgct tgagactata
     5341 gatctgctct tcgagcttgc tgagctgcga gcctaagggt ttctcgactt cttc
//
LOCUS       DASULZ010000005         2151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1421211, whole genome shotgun sequence.
ACCESSION   DASULZ010000005 DASULZ010000000
VERSION     DASULZ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421211"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="VFY96_00080"
     CDS             <1..958
                     /locus_tag="VFY96_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5604876.1"
                     /translation="RRVGLLELHCSHRGTSLEFGRVEDQGIRCCYHGWLYDTAGRCLE
                     QPAEPADSTFKDRIHHPAYKVREIAGFIFAYLGPDPAPLLPRYDLFLQENGERVIGAG
                     TEYCNWLQRAENSVDQTHLVALHAPEYPQMALKTPEIGWQKTVYGAKITMHVPGVSKP
                     KHSHWVFPSHTRHTTARKDRVPDHAIRFRVPTDDTTTKTFWLRFTPNDEANRGRPLRL
                     KTVGFEDDKPGVYTRVDDGWWNIASHDQDRVAQESQGEIYDRSKEHLGASDEGVILLR
                     QTIKDSIDAVRQGRDPFWILRKPEENNRINFDASMAEIGALG"
     gene            complement(988..>2151)
                     /locus_tag="VFY96_00085"
     CDS             complement(988..>2151)
                     /locus_tag="VFY96_00085"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5604877.1"
                     /translation="LREIDNAALVLLITAFGTIESAVEAMRAGAFHYVTKPFHNEEIL
                     IQVKRAVEQKRLEQEVERLRTEVRAHHRFQNIIGQSAAMQKILETVAQVSDLPANTLI
                     EGESGTGKELIARAIHANSSRARGPFIAVNCAAIPETLLESELFGYVRGAFTDARKNR
                     SGLFREANGGMLFLDEISELPMTLQTKFLRVLEDKEVRPLGANQGEKVDTRVLSASNR
                     NLAELVRTDKFRQDLYYRLNVIRIELPPLRQRGDDVPLLVQHFVEKFAAGAKRQVDGI
                     EPEALAALKNYDWPGNIRELEHTIERAVLLGKGSRIGLDDLPPNVVADGQSSVVLAQA
                     LTRQLTLRDLEREYVAKILEATDGNKTEAAKILGVDRTTLYRKLEEYKLKDDP"
BASE COUNT          449 a          634 c          595 g          473 t
ORIGIN      
        1 gcggcgtgtc ggattgcttg aactgcattg ctcccatcgc ggcacctccc tcgaattcgg
       61 ccgggtcgaa gatcagggaa ttcgctgctg ttatcacggc tggctgtacg acacggcagg
      121 ccgttgtttg gagcagcccg ccgagccggc ggacagcaca ttcaaagatc gcatccacca
      181 tccggcctac aaagttcggg agatcgccgg ttttatcttc gcttacttgg gtcctgatcc
      241 tgcgccgctc ttgccccgtt acgatttatt cttgcaagag aatggcgaac gggtcatcgg
      301 cgccggcacg gaatattgca attggctaca gcgcgcggag aattcggtgg accaaacgca
      361 tctcgtcgcg cttcatgctc ccgaataccc gcagatggcg ctaaaaacgc cggagattgg
      421 ctggcagaaa actgtgtacg gcgccaagat tacgatgcac gtgccggggg tatccaagcc
      481 aaagcactcg cattgggtgt ttccatcgca cacccggcat acgacggccc gcaaggaccg
      541 ggtaccggat catgccattc ggtttcgagt gcccaccgac gacacgacga cgaaaacttt
      601 ctggttacgt tttacgccga acgacgaagc gaaccgcggg cgtccgctaa ggttaaagac
      661 ggtcgggttc gaggacgata agccgggagt ttacactcgc gttgacgacg gctggtggaa
      721 catcgcgtcc catgatcaag accgcgtcgc ccaagaatcc cagggcgaga tttacgatcg
      781 cagtaaggag catctgggcg cctcggatga aggcgttatc ttgctgcgcc aaaccatcaa
      841 agactccatc gacgccgtgc ggcaaggacg ggatcctttc tggatcctcc gcaagcccga
      901 agaaaacaac agaatcaatt tcgatgcgag tatggccgaa atcggcgctc tgggttgagc
      961 cgcaagacgg ccggccgcgc aacaacgtta cgggtcatct tttagcttgt actcttccag
     1021 tttgcggtag agcgtggtgc gatccacacc gaggatcttc gctgcctcgg tcttatttcc
     1081 gtcggtcgct tccaagattt tggcgacgta ttcccgttcg agatcgcgta atgtgagctg
     1141 ccgggttagc gcctgagcca aaacgaccga cgactgtcca tcagcgacga cgttcggcgg
     1201 cagatcatcc aatcctatcc gagatccctt gcccaaaagc accgcgcgct cgatcgtgtg
     1261 ttcgagctcc cgaatgttac ctggccaatc ataattcttg agcgcggcca gagcctccgg
     1321 ctcgatgccg tccacctgac gtttcgctcc ggcggcaaac ttctcgacga aatgctgcac
     1381 cagtagcggc acgtcatcgc cgcgctgacg cagtggcggc agctcgatcc gaatcacgtt
     1441 cagccggtag taaagatctt gacgaaactt gtcggtgcgg acgagttctg cgagatttcg
     1501 attgctcgcg gaaagcacgc gcgtatcgac cttttctcct tgattcgcgc cgagaggtcg
     1561 gacttcttta tcttccaaaa cgcgtaggaa tttagtctgc agcgtcatcg gcaattcgct
     1621 gatctcgtcc aaaaaaagca tgccgccgtt tgcctcgcga aataggccgc tgcgattctt
     1681 gcgcgcatcc gtgaatgcac cgcgcacata gccgaacagc tcactttcga gcagcgtttc
     1741 cggaatcgcc gcgcagttca ccgcaatgaa gggcccgcgc gcccgggaac tgttggcatg
     1801 aatcgcccgc gcaatcagct cttttcctgt accgctctcc ccttcgataa gggtattggc
     1861 gggtaagtca ctcacttgcg cgacggtttc gaggattttc tgcatcgccg cgctctgacc
     1921 gataatgttc tggaatcggt gatgggcccg tacttccgtg cgcaggcgct caacctcttg
     1981 ttcgagtcgt ttttgttcga ctgcgcgttt gacctgaatt agtatttctt cattatgaaa
     2041 cggcttcgtc acatagtgaa acgcgccggc acgcatcgcc tcgaccgcgc tttcgatggt
     2101 gccgaacgcg gtgatcaaaa gcaccaaagc agcgttatca atctcgcgca g
//
LOCUS       DASULZ010000006         3638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_203569, whole genome shotgun sequence.
ACCESSION   DASULZ010000006 DASULZ010000000
VERSION     DASULZ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203569"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="VFY96_00090"
     CDS             <1..1037
                     /locus_tag="VFY96_00090"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX5604878.1"
                     /translation="FIGTLAGTSLIKGLLAGGIGLMLAAIGVDPQTGSLRYTFGSLYL
                     WDRLDLIPVVVGLFAIPEIVDLAVRGTSIASQDQGKLTGVMEGVKDTFRHFWLVVRCS
                     LIGVYFGVLPVLGANVAQWISYAHAVQGLKDKSRAGKGAVEGLLGPGAANNSTRGGDL
                     IPTLAFGIPGSGSMALLLGAMLIVGLNPGPEMLKSRLDVTFSMVWVLIISNIIVAGLC
                     FMILNHLAALTFVRGSLLIPFLMFLVFIGSFTANNDLNDIITTLLFGFIGFCMVKFGW
                     PRPPLILGLTLGKIAENYLWISTAAYGAKWLTFPTVVGLIALTIFTVAYPTIKERIGK
                     AKYERPEDAV"
     gene            1434..3101
                     /locus_tag="VFY96_00095"
     CDS             1434..3101
                     /locus_tag="VFY96_00095"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5604879.1"
                     /translation="MAHDVVIKNGTVVDGTGSPAFSADVAIDGDKITAIGKNLGSGKK
                     EIDAKGHVVAPGFIDSHTHMDAFVVQYPNGNPVVNYGVTSIVIGDCGASCAPVPPKAE
                     PRQVLVQYLRRVLDKYVDDKDWRWNTFGDYLNYLEGKVAINVLPLMPHSPVRLTVMGE
                     AAYQRAANPEELDAMKNMVREGMELGAIGFSTSPRGGPAIHAGTPSTFADHDEIIELA
                     NVAGEYGGCFQFNGFQMILQPESGVPEMLTKIKGLQIGNEFRVRPGAKAAGMQSIKYM
                     EEAQKRGQDLCGVVIPYQHIRRFNFDDCFLFNGLPTWEAIKNSSDIKTKLQDKEIRRK
                     IEQERIACQGKLEFPEWHGWDRVVFEHLEKPELKKLELKNVEEISRMTEKPPVDAFFD
                     TWIEDNFNSAICYHGLANAHPELLAEMIKSDTSLIGTDVGAHLDRFFWHGAPTKILGY
                     WTREKHLFSLEQAVHKITGYPAKKLRLNRGVLKEGMPADVTVFDADKIDDLVSKKLPD
                     TVDAQEVRRHPPGMKAVVVNGATVVEDGHCNDVFPGKVRRQQLCLAN"
     gene            3201..>3638
                     /locus_tag="VFY96_00100"
     CDS             3201..>3638
                     /locus_tag="VFY96_00100"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5604880.1"
                     /translation="MAASAIDIHHHYVPQSLLEEAKKNGKHLGVELVEKDGQQSLSFA
                     GGPPFVLHPELPAIDKRLQMMTNSELAMAALEAHTATLGYRLTGEQGENWCNVYNDGI
                     HELVKRYPDRFVGLASVPLQDPPRAAKVLERAVRDLNFRGGYIG"
BASE COUNT          798 a         1002 c         1009 g          829 t
ORIGIN      
        1 cattcatcgg cacgcttgca gggacatcgt tgatcaaagg acttcttgcc ggaggtatcg
       61 gcctcatgtt agcggctatc ggcgtcgatc cccagaccgg ctctctgcgc tacacattcg
      121 gctcgctata cctgtgggat cgtctcgatc tcatccccgt ggtcgtgggt ctattcgcta
      181 ttcctgaaat tgtcgacctg gctgttcgcg gcacaagtat cgcttcgcaa gatcagggaa
      241 agcttacggg cgttatggaa ggcgtcaaag acaccttccg ccatttctgg ctggtcgtgc
      301 gctgtagttt gatcggagtt tatttcggag ttttgcccgt gctcggcgct aacgtggctc
      361 agtggatttc ttacgcccac gccgtgcagg ggctgaaaga caaatcccgc gccggcaaag
      421 gcgcggtcga gggcttgctc ggtcccggcg cagccaacaa ttcaacccgc ggcggcgatc
      481 tcattccaac tctcgccttc ggtattcccg gcagcggcag tatggcgctg ctgctcggcg
      541 cgatgctgat cgtcggcctc aatccaggcc ccgagatgct caaatcgcgg ctcgacgtga
      601 ctttttcgat ggtttgggta ttgattatct ccaacatcat cgtcgccggc ttgtgcttca
      661 tgattttaaa tcatctcgcc gcactgacct ttgtgcgagg ttccctgctg attccatttt
      721 tgatgtttct ggttttcatc ggctcgttca ccgcgaacaa cgatctcaac gacatcatca
      781 ccactcttct gttcggcttt attggatttt gcatggtgaa gttcggctgg ccgcgcccgc
      841 cgttaattct cggcctcacc ctgggtaaga ttgcggaaaa ctacctatgg atctccaccg
      901 ccgcttacgg agcaaaatgg ctgacatttc caaccgtcgt cgggctcatt gcactgacga
      961 tctttacggt ggcttacccg acgatcaaag agcggatagg taaagccaaa tacgagcgtc
     1021 cggaagacgc tgtctaattc ctcgccacct tggcattttc aagaacctca cgtctcgcgt
     1081 tcacaagccg gagacgcttt tttcccgcaa tcatcggccc cttcacctaa gatccggcga
     1141 aggcggacgc ggacactgga ccaggcggga gagagctctc accttaggat cgagatggag
     1201 gattatgtac ttgtcagtca aatttttcgg tggccctcag gatagactcc gcgaggaatc
     1261 tttttccatg ctaagagtca tggtgaattg aaatgaacca acacccggac gcaatttcat
     1321 tcccattcca aaactgtgac acgagttaat actcaattga tccgccagtt gacagcctca
     1381 ccgtttaggt gggataagaa tttcaacgac cgagcatagg aggagatccg attatggctc
     1441 atgatgtagt catcaagaac ggaaccgtcg ttgacggcac gggcagtccg gctttttcgg
     1501 ctgatgttgc gatcgacggc gacaaaatca ccgcgatcgg aaagaatctc ggctccggca
     1561 aaaaggaaat cgatgccaag gggcatgtgg tggcgccggg gtttatcgac tcgcatacgc
     1621 acatggatgc gtttgtcgtg cagtacccga acggcaatcc ggtggtcaac tacggcgtta
     1681 ccagtatcgt tatcggcgat tgcggcgcat cctgcgcgcc cgtgccgccg aaagccgagc
     1741 cgcgccaggt tttggtccag tatctccgtc gcgtgctcga caaatacgtt gatgataaag
     1801 actggcgttg gaatactttc ggcgattatc tgaattacct ggaaggcaaa gtggcgatca
     1861 acgtgctgcc gctcatgccg cactcgccag tgcgtctgac ggtgatgggt gaagccgcct
     1921 atcagcgcgc cgccaatccg gaagagttgg atgccatgaa gaacatggtg cgcgaaggca
     1981 tggagctcgg cgccatcggt ttttccacca gtccgcgcgg cggaccggcg attcacgcgg
     2041 gcacaccgag cacgttcgcc gatcacgacg aaatcatcga gcttgccaat gtcgccggag
     2101 aatacggcgg ctgctttcag ttcaacggat ttcagatgat tctccagccg gagtccggcg
     2161 tgccggagat gctgaccaaa atcaaaggcc tgcagatcgg aaatgaattc cgcgttcggc
     2221 ccggcgccaa ggctgcgggc atgcagtcga tcaaatacat ggaagaagcg cagaagcgcg
     2281 ggcaggacct ttgcggcgtg gtcattccct atcagcacat tcgccgattc aatttcgacg
     2341 actgcttttt gttcaacggc ctgccgacct gggaggcgat caaaaactca tccgacatca
     2401 agaccaagct gcaagataaa gagattcgca gaaaaatcga acaggagcgt atcgcctgtc
     2461 aaggcaagct cgaatttcca gaatggcacg gttgggaccg ggtggttttc gagcatttgg
     2521 aaaaacccga gctgaaaaag ctcgagctca agaatgttga agagatttcc cggatgacgg
     2581 aaaagcctcc ggtcgacgcc tttttcgata cgtggatcga agacaacttc aattcggcaa
     2641 tctgctatca cggcctggcc aatgcccatc ccgagttgct cgcggaaatg atcaagtcgg
     2701 acacgagcct catcggcacg gacgtcggcg cgcacctcga ccgcttcttc tggcatggcg
     2761 ccccgacgaa aattctcggc tactggacgc gcgagaagca ccttttcagt ctcgagcagg
     2821 cggtgcacaa aattaccggc tatccggcaa agaaactccg cctcaatcgg ggtgttctga
     2881 aggagggcat gccggcagac gtgacggttt tcgatgccga caagatcgac gacctcgtga
     2941 gcaaaaagct tcccgatacg gttgacgccc aggaagtgcg gcgccatccg ccgggcatga
     3001 aagctgtcgt cgtcaatggc gctacggtgg tcgaagacgg ccactgtaac gacgtcttcc
     3061 ccggcaaagt gcgccgccag cagctctgtc tagccaacta aatctttttg tctttgtttt
     3121 cttgccttca tctcaccctc tccctcggga gagggctggg gtgagggaaa gtctttgatt
     3181 taaatttcga gaggatcgcc atggccgcgt ctgccataga tattcatcat cattatgttc
     3241 cccagagtct tttggaagaa gccaaaaaga acggcaagca tctcggggtg gagctggtcg
     3301 agaaagatgg acaacagtcg ttgtcattcg ccggcggtcc gccatttgtt ttgcatccag
     3361 agcttccggc gatcgataag cgacttcaga tgatgaccaa ttccgagctc gcgatggcgg
     3421 cgctggaagc gcataccgcc acgctcggtt atcggctcac cggcgaacag ggggaaaatt
     3481 ggtgcaacgt ttacaatgac gggattcatg agctggtaaa gcgctatccc gatcgttttg
     3541 tcggtttggc atccgtgccc ttgcaagatc cgccgcgcgc cgcaaaagtt ctggagcgcg
     3601 ccgtgcgtga tttgaatttt cgcggcggct acatcggt
//
LOCUS       DASULZ010000007         3335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1421348, whole genome shotgun sequence.
ACCESSION   DASULZ010000007 DASULZ010000000
VERSION     DASULZ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421348"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VFY96_00105"
     CDS             complement(<1..717)
                     /locus_tag="VFY96_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5604881.1"
                     /translation="MAVDQVDLEIPSGVCFGVLGPNGAGKTTTLRMIYGVTQPTSGTI
                     HVFGMDITKNLRAVRSRLGVTLQQNVLIEALSPKENLQVFGRYHLLREPELSRRAEQL
                     IDFLELRSHAEVPVRQLSGGFQRRLSIALSLINAPELLILDEPTTGLDPAVRLALWSR
                     VRELREAGTTVLITTHYMDEAQRLCDQVAIIAAGKVIASGAPAELIVSRLAPETVEFD
                     FTPNEEALLFDGFEPKRRLRI"
     gene            complement(977..1954)
                     /locus_tag="VFY96_00110"
     CDS             complement(977..1954)
                     /locus_tag="VFY96_00110"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5604882.1"
                     /translation="MRFVIALSGLALLCAGEQAGAQSKVRLNWGAISGVMSPIWVAQE
                     EGIFKKYGLDVELIHIAATSKAIQSMLSGEIQFTTADALNTIQAVNAGADLVMVCAGV
                     NRFVFSIMARGDIKRISDLKGKKIGITRIGSSTHTAVLYVINKAGLGANDYTLLQLGE
                     VPNILTTLLAGQIDAGALSPPTNSRAKKAGLQELINLATDGPEYPSTVIASTRSYVKS
                     NPENTRRIVRALGEGLAIFKSNRQIGIRAIQKYARLKDAEILEDTYNQFRDAFDAIPY
                     VSRAGIASLLASMGEKDPKIHQIKYEDVADMRFVAEAEKEGVFKKLAIK"
     gene            complement(2053..3129)
                     /locus_tag="VFY96_00115"
     CDS             complement(2053..3129)
                     /locus_tag="VFY96_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5604883.1"
                     /translation="MAESLHYYIAHNGGLKVMENRSGALQTVGEFFAGKTLEHLIGCR
                     KKPEIVFAAVAFDGGYRSDDAGKSWTKIMEGDVRTFTVDPHDERVVYMGMGPIRLLRS
                     EDKGNTWEPLDGMLAIPEEAKKKWDVPPRLRGVEFPHVRHIFIHPDDQSLIYVLLEHG
                     GVLMSRDRGNTWYDRSIGIGYVDMHYIENYPGSKERYYVSSARGFYRSDNSGEHWRRV
                     ENGMPWGYTELHSYSHEWHFMPGDTPRMVLCGGKGSPGVWSREKIHPHGCILLSDDAG
                     ENWRVSTQGLEKENPWMPWVLLHHPTDSNALFCGMGDGARGYGFDARIGGKGALYMSR
                     DRGDSWEPVIQNTPSILTAWVAPN"
BASE COUNT          715 a          990 c          857 g          773 t
ORIGIN      
        1 gatacgcagg cgccgtttcg gttcgaatcc gtcgaacagt agtgcctctt catttggcgt
       61 gaaatcgaac tccacagttt ccggtgctag ccgggacaca atcaactcgg caggagctcc
      121 cgaggcgatc accttgcccg cggcaatgat cgcgacttga tcgcagagcc gctgcgcctc
      181 gtccatgtag tgcgtcgtga tgagcaccgt cgttcctgcc tcgcgtaatt ctctgacccg
      241 cgaccacaag gcgaggcgca cggcaggatc gagcccggtg gtgggctcgt cgagaatcaa
      301 cagttcgggc gcattgatca gcgagagcgc aatcgacaac cgccgttgga atccgcctga
      361 aagctggcgc acgggaacct cggcatgtga gcgcagctcc aaaaaatcga tgagctgttc
      421 agcccgccgc gaaagctccg gctcgcgcaa gagatgatag cggccaaaca cttggagatt
      481 ttctttgggt gacaaagcct cgatcagtac gttctgctgc agtgtgacgc cgagccgtga
      541 acgcaccgcg cgcaagtttt tagtgatgtc catgccgaat acgtgaatcg taccactcgt
      601 tggctgggtc actccgtaaa tcatccgcag agtggtggtt ttccccgcgc cgttcggacc
      661 gagaactccg aaacaaaccc ccgacggaat ctcgaggtca acctgatcga cggccactcg
      721 atcgccgaat ttcttcgaaa gttcgcgcgt ccccaccacc attgttgcgg atgaagcctg
      781 ctgtttcacg atgttcttgg gaacctgttc gatgtgctag ttgattaagt cctttgaacg
      841 gcgtcacgca ccccgtgaac atacgcatga cggggaaggc aaaaaacgat ctctgtcatg
      901 gagaatcccg aggcgaaaca gatccaccat aggtgatgtt atcgtccgaa ctggcaagaa
      961 taaagatgaa cgtggattat ttgatggcga gtttcttaaa cactccttct ttttccgcct
     1021 ccgccacaaa gcgcatatcc gcgacgtctt cgtacttgat ttggtgtatt ttcgggtcct
     1081 tttcacccat gctcgccagg agtgatgcga tcccggccct gctgacgtag ggaatcgcgt
     1141 caaacgcgtc acgaaactga ttgtaagtgt cttctaaaat ttccgcgtcc ttcaaacgcg
     1201 cgtatttctg aatcgcccgg atgccgatct gccgattgct tttgaagatc gcaagtccct
     1261 cgccgagagc gcgaacgatg cgccgtgtat tttctgggtt gctcttcacg taggaccgag
     1321 tgctagcgat aaccgtcgac ggatactcag gcccgtcggt agcgagattg atcagctctt
     1381 gcaagcccgc tttcttcgcc cggctattgg ttggcggcga aagcgctccc gcatcgattt
     1441 gtcccgccag caaggtcgtt aaaatgttcg gcacttcacc gagctgtaac agcgtgtaat
     1501 cgttggctcc gagtcccgct ttgttgatga cgtacagcac cgcagtgtga gtcgacgatc
     1561 caatacgcgt gatgccgatt tttttgcctt tcagatctga gattcgcttg atatcgccgc
     1621 gagccatgat tgagaagaca aaccggttga cacccgcgca aaccataacc aaatccgcgc
     1681 ccgcgttcac cgcctggatc gtgttgagtg catccgccgt ggtgaactga atttcgcccg
     1741 acaacatcga ttggatcgct ttggaagtgg cggcgatatg aatgagctct acgtcaaggc
     1801 cgtacttttt aaatatgccc tcttcctgcg ccacccagat ggggctcatc acaccgctga
     1861 tcgcgcccca gtttagacgg actttgctct gcgccccggc ctgctctccc gcgcataata
     1921 gcgccaagcc gctgagagcg atgacaaatc gcattcggct cttcgcaacc gtgaatcgtt
     1981 tatttatcaa tcgaagtgtc gtcaagaagg ttttgattta gatgggtagc cgtttcttgt
     2041 tggagttttc gttcaattcg gagccaccca cgcggtcaag atcgacggcg tgttctggat
     2101 caccggctcc caggaatctc ctcggtcacg gctcatgtaa agcgctcctt taccccctat
     2161 acgcgcatca aacccgtagc cgcgcgcgcc gtctcccatg ccgcagaaaa gtgcgttcga
     2221 atcggtcgga tggtgcagca gcacccaggg catccaggga ttttcttttt ccaatccttg
     2281 cgtagagacg cgccagttct cgccagcatc gtcactcagc aaaatacatc catgcgggtg
     2341 aattttttcc cggctccaaa ctcccggcga gcccttgccg ccgcacaaaa ccatacgcgg
     2401 cgtatccccg ggcatgaaat gccattcatg agaatagctg tgcagctcgg tgtagcccca
     2461 gggcatgccg ttctccacac gccgccaatg ctcgccgcta ttgtcactcc gataaaaacc
     2521 gcgcgccgac gatacatagt agcgctcttt gctgccggga taattttcga tgtaatgcat
     2581 atcgacgtag ccgataccga tgctgcggtc gtaccaggta ttgccccgat cgcggctcat
     2641 taaaacaccg ccgtgctcga gcaagacata aatgaggctc tgatcgtcgg ggtgaataaa
     2701 gatatggcgc acgtggggga attcgacacc acgcaggcga ggcggcacat cccacttctt
     2761 tttggcttct tcgggaatgg cgagcatgcc gtcgagcggc tcccaggtgt ttcccttatc
     2821 ctcactgcgc agcagccgga tcggccccat tcccatgtaa acgacgcgtt catcgtgggg
     2881 atcaaccgtg aatgtccgca cgtctccttc catgatcttc gtccagcttt tcccggcatc
     2941 gtcactgcga tagccgccat cgaaggccac cgcggcgaaa acgatctcag gttttttcct
     3001 gcagccgatc agatgctcca atgttttgcc ggcgaagaat tctccaaccg tttgcagtgc
     3061 gccggagcga ttctccatca ctttcaatcc accgttatga gcgatgtagt aatgcagcga
     3121 ttcggccata agtctcctcc ctttccctcc ctaacgctaa actgtggagt tcaattccga
     3181 aatacaagac actacactaa acgccagacg cttttccgtt cttgagcgcc ctataaactt
     3241 tctccgaagt caccggcaga tccgtaatgc gcacaccgca agcgtccgct acagcattcg
     3301 caaccgccgc ggcggttggc acattggcga cctcg
//
LOCUS       DASULZ010000008         7684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_203766, whole genome shotgun sequence.
ACCESSION   DASULZ010000008 DASULZ010000000
VERSION     DASULZ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7684
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203766"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VFY96_00120"
     CDS             complement(<1..774)
                     /locus_tag="VFY96_00120"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5604884.1"
                     /translation="MTSNNRYVILGLFLLLLSAPQSDGADKITLSSTNPTAFEIDHVV
                     AKEKGFFAKENLDVEVNYMTPDLVVKALIAGTVDIARSGSHFGLIAATRGAEIKIIGG
                     SNYGYPYQVIGNPQFKTLADLKGQKIAGASLASITTTIFKDVMQRRGIPPTAYTLLFV
                     GGSPERFQAVASGQVAASLAEAPPFNFKSIEAGRKVLLNYSDEIKNLQYTAFFAASKS
                     LTQNRQLFTRFIRAIGQGMRWVNDPANEKAAIELMVQRLK"
     gene            complement(1100..2161)
                     /locus_tag="VFY96_00125"
     CDS             complement(1100..2161)
                     /locus_tag="VFY96_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007260895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX5604885.1"
                     /translation="MHRIPIDVPLLQEKIVTSILFTTIETDDGISGYGLTRGSQRFGL
                     KEFINREAGPFLRGKNPLETERIWNQLYKQFNPRAQTGMWSSAVSAIDIALWDIKGKR
                     YREPVWRLLGGAQNPVQAYVTFGLKQYEKDHLTEVAKQLVERGETRLKMVVAVDPEKP
                     KLDAERVRAVREAVGDEVELMIDANYLFSFNRALELCKLVEPYGITWFEEPVYQNDAK
                     LLADLRRHTDIPLAAGQNEGHRFRHRELIVNHAVDIAQPNVCSVGGYTEAVKVAALAQ
                     AFNLPIANGGGWPHHNMHLQAAMANGWRVEFHFEMWGVGDKIYKEPAAPDHGWVMLPE
                     TPGLGLEPRWDVLKEYEEK"
     gene            complement(2405..3448)
                     /locus_tag="VFY96_00130"
     CDS             complement(2405..3448)
                     /locus_tag="VFY96_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5604886.1"
                     /translation="MSITVIFAILSLWALSTGSSFAQSQFFAGKTITVVLGGPPAGSA
                     DMRTRAVTNLLRKHIPGNPNIVIQYMPAGGGRQAANHVFNVAKPDGLTLGSMGAALVA
                     NAVLGEPGVQYDIDKFIYLGTPDSAQHYLFITHRDAGLNTLEKLRAAPAVRVGGQSVG
                     HPVYITGRLFAYMLGLNDPRFVTGYTGPQLDIAITRREIDARVTTGETMLVRSREWLD
                     KGMVDILAGIEIPKGKKNARFAALPELESFARSNREKRLLEMFRAFRLAGQNFFVPPA
                     TPKERAEILQEAMRKTLTDPEFEKEYERTAGDTPSPLAPETLQKVIRDLPRDAATVEV
                     FNKLAGADPLPPR"
     gene            complement(3801..4700)
                     /locus_tag="VFY96_00135"
     CDS             complement(3801..4700)
                     /locus_tag="VFY96_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5604887.1"
                     /translation="MAIWIIGLSLLSIVFGHANLFAQPTLKKVRMGIQSTNIGFLPFH
                     IAFHKGFYREQGIDLEMIFMSTQAVNAAFVRGDLDYSAAVNGIIQGIVRGAPAKILAC
                     AVDRPLQSFISRKDIRGPRDLKGKKVAGSTTGGTATLMADAALKHFGLESGRDVSVIP
                     LRDNRLTALESGTVDAALLGVPENIIAVDKGYNELLFLGDVLSFPQNGFGASTKQRNR
                     DEVINIILKQWRLTDRRLAGEMLKQFGRGIIRDMSVRSEGIQLMIDLVRDDAKIAQPF
                     SVSQVADFSFLEKALRELGVTKP"
     gene            4912..5937
                     /locus_tag="VFY96_00140"
     CDS             4912..5937
                     /locus_tag="VFY96_00140"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5604888.1"
                     /translation="MRLNDKICARIVCCLFIFYCAICFLPSAKAQTIDETYNQALKEG
                     GVLNVYGTLTPDTAVKVLPVFEKRFPGIKTVNTGASSDKIVARAISEARGGRTIGDVF
                     HMNLENVIQVHEQGLVLEKLPPEAEVFATHLKGTYWVATRFNTLVAAWNTQLVSKADE
                     PKTFEDFADAKWKGKLLAEPRDAEILIAFAEQKYKNADNAIALFKKIAANSVEFHSGH
                     PQLVELLMAGQGSVCLTCYTHHLLPRMQKGAPLNYLRNLGVGLISATAVFKNAPHPNT
                     AWLFHRWAASDEGQKTFGIEGRIPANPKVEPAEPIGVTTVYGLGEKEYREFTKYEKIW
                     KQIFSLR"
     gene            5982..6392
                     /locus_tag="VFY96_00145"
     CDS             5982..6392
                     /locus_tag="VFY96_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEX5604889.1"
                     /translation="MATRWLFKTEPSVYSYQQLEKDKKTVWDGVANNLALKNLNDIKK
                     GEQIFIYHTGDEKAAVGVARALGGAYPDPEKDDPKMLVVDVEPVRALKRPVTLAEVKA
                     HLKLKNFDLVRNSRLSIMKVSDEQWEIMESMAKN"
     gene            complement(6389..>7684)
                     /locus_tag="VFY96_00150"
     CDS             complement(6389..>7684)
                     /locus_tag="VFY96_00150"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5604890.1"
                     /translation="RFHDSHLQHVTSYSADPHTRPMGIGLELSARRKDGTEFPVDIML
                     SPINSGKDGMVMAVIRDITEPKRTAEGVRKTNDELMALVTELQRRDREMQSLISMDDL
                     LQSCTSQEDAYKVISFAAGELFEGQTGCLAVLQASSRYLEMVARWGATPLGEPIFSLD
                     DCWALRRGQLHEVANAETGLLCRHFVSQRNTRYLCVPLTVQGEILGVFCLAGAPAHTS
                     DHQIGQLQLAVTVSESIKLSLSNLKLREKLRAEAIHDPLTGLFNRRYLEETLVRELHR
                     ARRRNSALCLTMLDLDNFKRFNDSHGHDAGDALLRELGQLMRAKLRKSDILCRYGGEE
                     FVIVLPDSSLVDAEQRIDQIRAQLKEVQIQHGGEFLPAPTLSAGIAQADEHNFDPGEL
                     LRAADKALYSAKNAGRDRVIRYGANAKGDRPGKKQPEDC"
BASE COUNT         1726 a         2073 c         2130 g         1755 t
ORIGIN      
        1 ttttaggcgc tggaccatca actcgatggc cgctttctcg ttcgctggat cattgaccca
       61 acgcatgcct tggccgatgg cgcgaatgaa acgggtaaac agttgtcggt tttgcgtcag
      121 tgatttgctc gctgcaaaaa acgcggtgta ctgaagattc ttgatttcgt ccgaataatt
      181 gagcagcacc ttgcgcccgg cttcgatgga tttgaaattg aatggcggtg cttccgccag
      241 cgaagccgcg acctgccccg aggctaccgc ttggaatctt tccgggctac cgccgacgaa
      301 caacagcgtg tatgctgtcg ggggaatccc gcgccgttgc atgacgtctt tgaaaatcgt
      361 cgtcgtgatg ctcgccaaag acgcgccggc gattttttgc cctttgagat cagcgagggt
      421 cttgaactgc ggattgccga tcacttgata aggataaccg tagttggagc cgccaatgat
      481 cttgatctct gcgccgcgcg tggcggcgat caagccgaaa tgcgaccctg accgagcgat
      541 gtccacagtc ccggcgatca gcgctttgac caccagatca ggcgtcatgt agttgacttc
      601 gacatcgagg ttctctttgg cgaagaatcc cttttctttg gccaccacgt ggtcgatttc
      661 aaaagcagtg ggattggtcg agctcaaggt gatcttgtcg gcgccatctg attgcggcgc
      721 tgagagcaaa agcaagaaca ggccgagtat cacgtagcgg ttgttgctcg tcataagttc
      781 ctccagttcg tggtaccgtt tatcattgtg aagcgtacgg aaaatcttta aagcctggat
      841 ttcgatccgc tacatcccgg ctacagtgta ctacaaactc taaagcctcg gattgcgtgc
      901 cgctccatcc gagctacgag cggtgcgcaa acgaaacgta cgagtggtaa agctgcgcat
      961 tggtgcgcaa gcgcacccta caatctgccc ccgcaaagaa ttccggctta ccacgaaccc
     1021 cacgaaggtc acgaaggaac tttggcaaga ccattgaact tcgtgttttt cgcgctgttc
     1081 gtggtgaaat acccagtggt catttctctt cgtactcttt cagaacatcc cagcggggtt
     1141 ccaagcccaa tccgggtgtt tccggcaaca ttacccagcc gtggtccggc gcggcgggct
     1201 ccttatagat cttatcgccg acgccccaca tctcgaagtg aaactcgaca cgccagccgt
     1261 tcgccatcgc tgcttgtaga tgcatgttgt ggtgcggcca gccgccgccg ttggcgattg
     1321 gcaggttgaa cgcctgcgcc agcgcggcaa cctttaccgc ttccgtatag ccgccgacgg
     1381 aacagacatt gggttgagca atatcgacag cgtgattgac aatcagctcg cggtggcgaa
     1441 agcggtgccc ttcgttttga cccgcggcga gcgggatgtc ggtatggcgt cgcaaatcgg
     1501 cgagcagctt agcgtcgttt tgataaacgg gttcttcgaa ccaggtaatg ccgtagggct
     1561 cgaccagctt gcagagctcc agagcgcggt tgaaggaaaa tagataattc gcgtcgatca
     1621 tcaattcgac ctcgtcgcca acggcctcgc gcacggcgcg tacgcgctcc gcgtccagct
     1681 ttggcttttc cggatcgacc gcgaccacca ttttgagacg tgtttctcca cgctcgacga
     1741 gctgcttggc cacttcggtg aggtgatctt tttcgtactg tttgagaccg aaagtaacgt
     1801 aggcttgcac cggattttgc gcgccgccga gcagacgcca gaccggctcg cgatagcgct
     1861 tccctttgat gtcccagagc gcgatatcga tggcactcac ggccgagctc cacatgccgg
     1921 tctgagcgcg cggattgaac tgcttgtaga gctgattcca gatccgttcc gtctcgagtg
     1981 gattcttgcc gcgcagaaac ggccccgctt cgcgattgat aaattctttc agaccgaatc
     2041 gctgcgatcc gcgagtcagc ccgtacccgc ttatgccgtc gtcggtttca atcgtagtaa
     2101 acaagatcga tgtgacgatc ttttcctgta agagcggcac atcgattgga atgcgatgca
     2161 aagttgcccg gacatcggct attttcagag gtcacctcca gaatgccggc gatgaatgtt
     2221 gctctaaatt tttgtatgtt caatcatcaa tgttgttcaa acgctcaaat cctcctgatt
     2281 ttgccgcaag gagacttatc agaatgacat ctatttgata ttggagcacg acagagattc
     2341 gacgccacga acattaaact atccgtcacg tgacgtcgtg tggcatccag ttggtccttt
     2401 caaattaccg cggcggtaac ggatcggcgc cagccagctt gttaaaaact tcgactgtcg
     2461 cggcgtcacg cggcaaatcg cgaatcactt tttgtagcgt ttcaggcgcg agcggcgagg
     2521 gcgtgtcgcc ggcggtcctt tcatattctt tttcgaattc gggatccgtg agagttttgc
     2581 gcatcgcttc ttgaagaatc tccgcacgct ccttcggcgt tgccgggggg acgaagaaat
     2641 tttggccggc aagtctgaag gcgcgaaaca tttcgagaag ccttttctca cgattggaac
     2701 gcgcgaagct ttcgagctcc gggagggcgg caaaccgagc gttctttttt cccttgggaa
     2761 tctcgatccc ggcaagaata tcgaccatgc ctttgtccag ccattcgcgc gaacgtacca
     2821 acatggtctc gccggtcgtg acacgcgcgt cgatctcgcg gcgagtgatc gcgatatcga
     2881 gttgaggacc ggtatagccg gtcacgaacc tcgggtcatt gaggccaagc atgtacgcga
     2941 acaggcgccc cgtaatgtag accgggtgac cgacggattg accgcccacg cgcacggccg
     3001 gagctgcgcg cagtttttcc agcgtattca atccagcatc acgatgcgtg atgaacaaat
     3061 aatgctgggc gctgtcgggg gtgccgagat agataaattt atcgatgtca tattgtacac
     3121 cgggttcccc taggacggca ttggcgacca aagcggcacc catgctgccc aacgtgaggc
     3181 cgtcgggctt agcgacattg aagacatgat tggccgcctg ccgaccgccg ccggccggca
     3241 tgtattgaat gacaatgttc ggattgcctg gaatgtgctt gcgtaggagg ttcgtgacag
     3301 cgcgggttcg catatctgcg gagcccgcgg gcggcccgcc cagaaccacg gtgattgttt
     3361 tgccagcgaa aaattgtgat tgagcgaagc tcgagcctgt gctcaatgcc cagagactca
     3421 agatggcaaa aatcacggtt atgctcataa aaggatctgc cgaagcgatg cgacaaagcc
     3481 ctcctactcc agtccaagtg aattgtccac cggtcagttt gcgaacgatc tctcaagtcg
     3541 ctcgatataa tagactgtaa tacagtcatt ccgagcaaag cgaggaatct tcttgaattc
     3601 taagcgtcga aataatttgt ggaactagga tggattcaat aattcattct ctgtcttgcc
     3661 gtcatagcgg cgaacgccgg tattcagaaa atgtaaaatt cctggattcc gggtcgcgct
     3721 agctatcgcc agctgcccgg aatgacgctc gaacagtatg gctaatcttc gattccgggg
     3781 atactaattt ttcgttgttt ttacggtttc gttacaccga gctcgcgtag cgccttttcg
     3841 agaaaactga aatcggcaac ttgcgaaaca ctgaagggct gcgcgatttt tgcgtcgtcc
     3901 cgtaccaggt cgatcatcag ctggatccct tcagatctga ccgacatgtc gcgtataata
     3961 ccgcgcccga attgcttgag catttcgcca gcgaggcgcc gatcggtgag ccgccactgt
     4021 ttcaaaataa tattgatcac ttcatctcgg ttgcgctgtt tcgtcgatgc gccaaagccg
     4081 ttctgaggaa aactcaagac gtcgcccagg aacagcaact cgttgtagcc tttgtcgacg
     4141 gcaatgatgt tttcgggtac cccgagtaat gccgcatcga ccgtgccgga ctctagtgcg
     4201 gtaaggcggt tgtctctgag gggaattacg gagacatcgc ggccggattc caaaccgaag
     4261 tgcttgagcg cggcgtccgc catcaaggtt gcggtcccgc ccgtggtgct gccagcgact
     4321 ttctttccct tgaggtcgcg cggcccccga atgtcttttc tgctaatgaa cgactgcagc
     4381 ggccggtcga cagcacaggc caagattttg gccggtgcac cacgcacgat accctgaata
     4441 atgccgttga ccgccgcgct gtaatcaaga tcgccacgca caaaagccgc gttcaccgct
     4501 tgagtggaca taaaaatcat ttccagatcg atcccttgct cgcggtaaaa tcctttgtgg
     4561 aaggcgatat gaaaaggcag gaagccaata ttggtcgact gaattcccat ccgaactttt
     4621 ttgagcgtcg gctgcgcgaa cagatttgcg tggccgaaaa caatcgacag aagcgataat
     4681 ccgatgatcc aaattgccat gtgatatgtg actgtttata accgagggaa ctccgcaggc
     4741 cgatatacgc cgtagcataa tttgccgcct atgaaaaaca tttggcacga cgcgttccgg
     4801 cgaaggaagt gaaggattaa cgataatgag ccattgccga tccggtcatt ccttgatatt
     4861 tcgaaaggcg ctttgctacg gaggaacacc attcacaatc ggagcggtct aatgcggcta
     4921 aacgacaaaa tctgcgcgcg catcgtgtgt tgcttgttta ttttctactg cgcaatttgt
     4981 ttccttccat ctgccaaagc ccagaccatc gatgagactt acaaccaggc attgaaggaa
     5041 ggcggcgtgc tgaatgtcta tggcacgttg acgcccgaca cggcggttaa agtcttgccg
     5101 gtcttcgaaa agcgctttcc aggcatcaag accgtcaaca ccggggcgag ctcggacaaa
     5161 atcgtcgccc gggcgatttc cgaagcacgc ggcggcagaa ccatcggcga tgttttccac
     5221 atgaacctcg agaatgtcat ccaggtgcat gagcaagggc tggtgctgga gaaattaccg
     5281 ccggaagccg aggtttttgc gactcatctg aagggcacat actgggtggc gacccgtttc
     5341 aatactttgg tggcggcgtg gaatactcaa ttggtaagca aagcggatga gccgaaaacc
     5401 ttcgaagact tcgctgacgc aaaatggaag ggaaagctcc tggccgagcc gcgggatgcc
     5461 gagattttga tcgcgtttgc cgagcagaaa tataaaaacg ccgacaacgc cattgctttg
     5521 ttcaaaaaga ttgccgccaa cagcgtcgag ttccatagcg gccatcctca attggtcgaa
     5581 ctcttgatgg ccgggcaagg ctcggtttgc ctcacctgtt acacccatca tctattgccg
     5641 cggatgcaga aaggcgcgcc actcaattat ctgcgcaacc tcggtgtcgg tttgatcagc
     5701 gcgacggcgg tcttcaaaaa tgcgccgcac ccaaatacgg catggctttt tcatcgctgg
     5761 gccgcttcgg acgaaggtca aaaaaccttc ggcatcgagg gccgcatacc ggccaatccg
     5821 aaagtcgagc ctgccgagcc gatcggtgtc acaaccgtgt acggcttggg cgaaaaagaa
     5881 taccgggaat tcaccaaata cgaaaaaatt tggaagcaga ttttcagctt gcgataggct
     5941 tcggcgatcg aactcacgta ggcatctcaa caggaggaag catggcaact cgttggttgt
     6001 tcaaaaccga accgagcgtt tactcctacc aacagttgga gaaggacaag aaaaccgtgt
     6061 gggacggcgt ggcgaataat ctcgcgctga aaaatctcaa cgacatcaaa aaaggcgagc
     6121 agatttttat ttaccatacc ggcgatgaaa aggcggccgt gggtgtggcg cgagccttgg
     6181 gcggcgcata cccggatccc gagaaggacg atcccaagat gctggtggtg gatgtcgagc
     6241 cggtacgcgc cttgaagcgg cccgtgactc tggcggaagt gaaggctcac ctcaaattga
     6301 agaattttga tctcgtacga aactcgcgct tgtcgatcat gaaggtcagc gacgagcaat
     6361 gggagatcat ggaaagcatg gccaaaaatt aacaatcttc aggttgtttt tttcccggtc
     6421 gatcgccctt agcgtttgcg ccgtagcgga taacacggtc gcgtccggcg tttttcgcgc
     6481 tatacaaggc tttgtccgcg gcgcgtaata attcgccggg atcgaagttg tgttcgtcgg
     6541 cctgcgcaat gccggccgag agcgtaggag ccggcaggaa ctcgccgccg tgctggatct
     6601 gcacttcctt cagctgcgcc cggatctgat caatgcgctg ctcggcgtcg acgagcgatg
     6661 aatccggcag aactatgaca aactcttcgc cgccgtagcg gcagagaata tcgcttttcc
     6721 gaagtttggc gcgcattagt tgtcccaatt cgcggagtag agcatcaccc gcatcgtgtc
     6781 catggctatc gttgaagcgt ttgaaattgt ccaaatcgag catggtgagg caaagcgccg
     6841 aatttcgccg ccgcgcgcgg tgtagctcgc gcaccaaggt ttcttccaga tagcgccggt
     6901 tgaacaatcc ggtgagcggg tcatgaatcg cttcggcgcg gagcttctcg cgcaatttaa
     6961 gatttgacaa ggagagcttg atcgactcgc tcacggtgac cgccagttgg agttggccga
     7021 tttggtggtc actggtatgg gctggtgctc cggcgagaca aaataccccg agaatttcac
     7081 cttggacagt caacgggacg cagagataac gggtgttccg ttgactgaca aagtgacggc
     7141 agagcaaacc ggtctcggcg ttcgcgacct cgtgcagctg accccgtctc agcgcccagc
     7201 agtcatcaag cgagaaaatt ggctctccga gcggggtggc gccccagcgc gcgaccattt
     7261 cgaggtatcg actagacgcc tgcaaaacag cgagacagcc ggtctggccc tcgaaaagct
     7321 ctcccgcagc gaagctgatg accttgtaag cgtcctcttg agaagtgcag ctctgcagca
     7381 gatcatccat gctgatgagg gactgcattt cccgatcgcg ccgttgcaac tccgttacca
     7441 aagccatcag ctcgtcattg gtttttcgta cgccttccgc tgtccgtttg ggttcagtga
     7501 tgtcgcgaat gaccgccatc accatcccgt ccttgccgga gttgatcggg ctcagcataa
     7561 tgtccacggg gaattcagtt ccatccttac gccgcgccga gagctccagg ccaattccca
     7621 tgggtcgagt gtgaggatcg gcgctgtaac tggtcacgtg ctgaagatgg ctgtcgtgga
     7681 agcg
//
LOCUS       DASULZ010000009         3081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1015642, whole genome shotgun sequence.
ACCESSION   DASULZ010000009 DASULZ010000000
VERSION     DASULZ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015642"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VFY96_00155"
     CDS             <1..888
                     /locus_tag="VFY96_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX5604891.1"
                     /translation="KKVLAVGAEAKRMLGRTPGSIVAIRPLKDGVIADFEITEAMLRY
                     FIQKVHNRRTLVRPRIIVGVPFGITEVEKRAVRESAESAGAREVYLIEEPMAAAIGSG
                     LPITEPTGNMIVDIGGGTTEVAVISLSGIVFSRSVRVGGDKMDEAIAQFIKRKYNLLV
                     GERTAELIKITIGSAYPSDEVQTMEIKGRDLVAGVPKTVVITDEEIRDSLLEPINQIV
                     EAVRLSLERTPPELASDIVDRGIVLAGGGALLRNLDILLREETGLPVMMADDPLTAVV
                     MGAGKALDEISLLREVAVH"
     gene            935..1765
                     /gene="mreC"
                     /locus_tag="VFY96_00160"
     CDS             935..1765
                     /gene="mreC"
                     /locus_tag="VFY96_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX5604892.1"
                     /translation="MLAFVRKNQVPLSSCFCLLLSIYILTAAARGQLKNEPLGALLMW
                     IMRPLQLASEGAVSWVKNFRDNYDTLAGFKSENERLRKRIQNLEVERQRLLEAEATNR
                     QLKALLDFRSRLPAGAITASIIANSANSLFQSCMIDKGSSDGVRKGMAVVTPLGVVGQ
                     VVGVTPDAAKVLLLTDPNSGIDVLVQRTRSRGIVSGSLDSGTVLKYVKRSEDVQEGDR
                     LITSGIDGIFPKGMMVGTVIKVRKQNIGLFQFIEVLPAVQTSRIENVLVVAGDSAGKS
                     "
     gene            1797..2309
                     /gene="mreD"
                     /locus_tag="VFY96_00165"
     CDS             1797..2309
                     /gene="mreD"
                     /locus_tag="VFY96_00165"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX5604893.1"
                     /translation="MRLFLLFFLVGILLILLQTTLLHLLPIGPIIPDLTLVLCVYLGL
                     YHPTVGAALGSFLIGYSVDVVSSPSRMVGLNAFAMSLVFLLVYFSSRSIWLQNPLVSS
                     IVVLFAALVKSAALVLISAFFVNLEGFWIGATWYIIQEALAAAVLAPVVFGLLKAGEN
                     YAARFRAAGQ"
     gene            2318..>3081
                     /locus_tag="VFY96_00170"
     CDS             2318..>3081
                     /locus_tag="VFY96_00170"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX5604894.1"
                     /translation="MAPLANISKQDGAPEVRRRSQLLFIFIAVAFLGLITRLVFLQIV
                     EGERFTYLSENNRIRIKRVPGTRGMIFDREGQLLVDSRPSFDLIFVPEDSERPETTLK
                     LLAGYLRRDPVELLKIFEENKSRAAFDELVLGRDIEWPAVVAVETHQLELPGVSLRAR
                     PRRNYSDGPMAAHVLGYLGEINQKQLKLLKDQGYVLGDEIGQYGLERRWEELLRGQSG
                     GQQVEVDALGRRVRVLHEVTDVPGYTVHLTLDRQMQ"
BASE COUNT          642 a          829 c          911 g          699 t
ORIGIN      
        1 aagaaagtgc tcgcggttgg tgccgaagcc aaacggatgc tcggccgcac accgggaagc
       61 atcgtggcca tccgcccgct caaggacggg gtgattgcgg attttgagat taccgaagcg
      121 atgctccgct acttcattca aaaggtgcac aaccgccgga ccttggtgcg cccgcgcatc
      181 atcgttggcg tcccgtttgg catcaccgaa gtggagaagc gtgcggttcg tgaatcggcc
      241 gagtcggctg gcgcacgaga ggtttattta atcgaggaac cgatggccgc cgctatcggt
      301 tcgggattgc cgatcacgga accgaccggc aacatgatcg tggacatcgg cggcggcacc
      361 actgaagtcg cggtgatctc gctctcgggc atcgtattct cgcgctcagt gcgggtaggc
      421 ggagacaaga tggacgaggc gatcgcgcaa ttcataaaac gcaagtacaa tctgctcgtc
      481 ggcgaacgca ctgccgagct catcaaaatc actatcggct cagcgtatcc cagcgacgaa
      541 gtgcaaacca tggagatcaa aggacgtgat ttggtggccg gcgtcccgaa aaccgtggtc
      601 atcaccgacg aggagattcg cgactcgtta ctcgaaccga tcaatcagat tgtcgaagcc
      661 gtacgcctct cactcgagcg cactccacct gagctcgcgt cggatattgt cgatcgcggg
      721 atcgtgctcg ccggcggcgg cgctttgtta cgaaatttgg atattctact gcgtgaagaa
      781 accggcctgc cggtgatgat ggcggatgat ccgttgacag cggtcgtaat gggagcgggc
      841 aaagccttag atgagatctc actactcaga gaggttgcgg ttcattagca gaggtccgca
      901 tgcattggga tgaccggagg caatcgagcc ttccatgctc gcttttgttc gtaaaaatca
      961 ggttccgcta agttcttgtt tctgcctact tctatcgatt tacattttga ccgccgcagc
     1021 gcgcggccag ctcaagaacg agccgctcgg cgccctgttg atgtggatca tgcggccgct
     1081 gcaactcgcg tccgaagggg cggttagttg ggtaaaaaat tttcgcgaca actatgatac
     1141 tctggcagga ttcaagtcgg agaatgagcg attacgcaaa cgtattcaaa acctcgaagt
     1201 cgaacgacag agattattag aggcagaagc cactaaccgc cagcttaaag cactcctcga
     1261 tttccgctcc cgtttaccgg ctggggcgat caccgcctcg atcatcgcca acagcgccaa
     1321 tagcttgttt caaagttgca tgatcgataa aggcagcagc gacggggtac ggaaaggtat
     1381 ggcggtcgtc acgccgctcg gcgtggtcgg gcaagttgtc ggcgtgacac ccgatgccgc
     1441 gaaagtcctg ctgctgaccg atccaaacag cggcatcgac gtcctggtgc agcggacgcg
     1501 cagccggggc attgtctcgg gatcgctgga tagcggcacc gtcttgaagt acgtgaagcg
     1561 aagcgaggat gtgcaagaag gcgaccggct gattacctcg ggaattgacg ggatttttcc
     1621 caaaggaatg atggtcggaa ccgtaatcaa agtacgtaag cagaacattg gcttgttcca
     1681 attcatcgag gtactgccgg cggtgcaaac ctcacgcatc gaaaacgtcc tggtcgtagc
     1741 cggggatagt gcgggcaaaa gctgagtttt gacgcgtaca ggtctagtaa ttcgctatgc
     1801 gcctcttttt gcttttcttt ctcgtcggga ttcttttgat tctgctccag acgacactgc
     1861 ttcacctcct acccatcggt ccgatcattc cggacttgac gttggttctg tgcgtctatc
     1921 tggggctgta tcatcctaca gtcggtgctg ccttgggctc gtttctaatc ggttattcgg
     1981 ttgatgtggt gtcgagtccg agccgaatgg tgggcctcaa cgccttcgcc atgtctttgg
     2041 tgtttctgct cgtgtacttc agctcgcgtt ccatctggct gcaaaatcca ctggtgagct
     2101 cgatcgtggt tttgttcgca gctctggtca aaagcgcggc cctggtgttg atctcggcat
     2161 tcttcgtcaa tctcgaggga ttctggatcg gcgccacttg gtatataatt caagaagctt
     2221 tggcggcggc ggtgcttgcg cccgtagttt ttgggttgct caaggccggt gaaaattatg
     2281 ctgctcgatt ccgcgccgca gggcagtaac tccgacaatg gctcccctcg caaacatcag
     2341 taaacaagac ggcgcgcctg aagtgcggcg gcgcagccag ttgcttttca tttttatcgc
     2401 ggtggcgttt cttgggttga tcacgcgtct ggtttttctc cagattgtcg agggcgagcg
     2461 gtttacctat ctctccgaaa ataatcggat ccgcatcaaa cgggtgcccg gcacgcgcgg
     2521 catgatcttc gatcgagaag gccaactcct cgtcgacagc cggccctctt tcgatctgat
     2581 ttttgtgccg gaagatagcg agcgtccgga aactaccctg aaattgctcg ccggttatct
     2641 gcgccgggat cctgtcgagc tgctgaaaat cttcgaggaa aacaagagcc gcgcggcatt
     2701 cgatgaactg gtgctggggc gcgatattga atggcccgca gtcgtagcgg tagagacgca
     2761 tcaactcgag ttgcccggcg tcagcttgcg cgcccgaccc cggcggaatt actccgatgg
     2821 acccatggcc gcgcacgtgc tcggttattt gggtgagatc aatcaaaaac agctcaagct
     2881 tctcaaagac caaggctatg tgctcggcga tgagatcggc caatacgggc tcgaacggcg
     2941 ctgggaggag ttactgcgcg gccagagcgg tggacagcag gtcgaagtcg acgcgctcgg
     3001 ccgccgcgtg cgcgtgctcc acgaagtcac cgacgttccg gggtatacgg ttcacctgac
     3061 tttggaccgc cagatgcagg a
//
LOCUS       DASULZ010000010         5695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1320208, whole genome shotgun sequence.
ACCESSION   DASULZ010000010 DASULZ010000000
VERSION     DASULZ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320208"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..1121)
                     /locus_tag="VFY96_00175"
     CDS             complement(126..1121)
                     /locus_tag="VFY96_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5604895.1"
                     /translation="MSIVKKTTFALLASAAIAFLFPAGSKAQSRKMIVGQAGVNPGAG
                     LFTIAVNQGFYKKHGLDVEIIKTNTTAAVQAMLAGKMQMATGAGAAAFVTATLEGAPP
                     FVLVASWVNVFPYKIMAQKGIKKIEDLKGKTGHVGAPFGTIPDTALRFALTRLKIDPE
                     KDVKLVQHSNADPASILAAMEKGEVQFGILAPPFDLIAERRGYEMLLALPGLGIPWQQ
                     NGELVPASYLKSNRDNVLRLLRATAEAEKFYFDQKDKTIEIMTEYLGRPRDETEYAYN
                     QYKLWTDRIPRPRVDTVTTTLEAIKKNTPKAASANAASFVDTTLVDQLLKEGYYK"
     gene            complement(1275..2150)
                     /locus_tag="VFY96_00180"
     CDS             complement(1275..2150)
                     /locus_tag="VFY96_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5604896.1"
                     /translation="MSLGVSRRAPTAKSKPDVVDLVSVDESNPLTTHRAWQRARQLIS
                     DWLGVIGIVLFFLAWELFTRLEILNPFYFPPFSKILAKGYELFASGAIWEHMWFSLTN
                     FAIGFVISVVLGVLLGVPMGWYKGVSKTFDPLLSGIYATPLIALLPLIIMLFGLGAIS
                     KIIMTVLAAVFPILINTMVGIANTDYRLITMARAFGAKDNQIFRKVSLPGSLPYIVAG
                     MRVALGRALVYIVVAEQYGAAMGLGYLSSVAAQRFQMAAMFVPIVIIAGLGAGLNELL
                     KSFERRLEKWKPPKS"
     gene            complement(2279..3076)
                     /locus_tag="VFY96_00185"
     CDS             complement(2279..3076)
                     /locus_tag="VFY96_00185"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5604897.1"
                     /translation="MLTPIKIENRMNAFLGIPAGDGKKPVVIHLHERYGIVQHTTDLG
                     QKFVDAGYVSIVPDYFSRFTGDRQKLAAGDDRCELDDEQVLVDTDAVVAYLRTVPQAD
                     MSKVAMSGVCQTGRQPILVGAERDYLSAAVVLYGAVYDADWKSHRVRPEPIDKLMQEI
                     KCPLTAVFGELDNLIHRDNILRMLTVLAGAKKSFDIRIYPDAPHGFLNDTMPGRYRRP
                     QSDAAWSQITSFLQMVFIGEQKRDRARWRFEADTSVNYDFSSMKRWE"
     gene            complement(3250..3825)
                     /locus_tag="VFY96_00190"
     CDS             complement(3250..3825)
                     /locus_tag="VFY96_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5604898.1"
                     /translation="MIINFYVIVAFSWLILAVSAMGANAPELTACSIVSGADAQKLVG
                     GELDVQEFAKVPTAGGADTYDSVCTYIAKGGSFQNPVGASRFLDVTLHFLNSADDMKT
                     VYDGSFDQYREMAKAADAPFKNAEITPVSGFGDKAFIVEAVTDVKTNYKSALIVFYKG
                     RTGGAVSAWRKPESSLETTKAVLRHILSKLP"
     gene            complement(3844..4911)
                     /locus_tag="VFY96_00195"
     CDS             complement(3844..4911)
                     /locus_tag="VFY96_00195"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5604899.1"
                     /translation="MAYALSGPPAFLPELVIGGMVNRICACFQTRVVFAMLLILSGAM
                     PASAQPRKINIAYTATSPYQAALIIAKEAGIFKKHNLDPQLILTPGGSLGFQAMMGGD
                     VAMVMADGSAAVTSNLAGADVVIIASFLNTFPYSLISIPEVKRVEQLVGGKVAISRFG
                     SATDVSVRMSLAKVGLNPDKDVTILQIGTQTTRFAALQSKQVQATIITPPFTLTARKQ
                     GYNTLIDMAQLNIPFELTALLTRKAYIKTQREIVLDVVAALADAVHFYKREKEPVLKI
                     LSRYLQTTDREALEETYREIALKTMPEKPYPTLPGIQTILDELAPKNPKAKASRPEDF
                     VDSSFVKKLDDEGYFERLYKK"
     gene            complement(4960..>5695)
                     /locus_tag="VFY96_00200"
     CDS             complement(4960..>5695)
                     /locus_tag="VFY96_00200"
                     /EC_number="4.1.3.34"
                     /inference="COORDINATES: protein motif:HMM:NF004868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HEX5604900.1"
                     /translation="ELDRIFVRNYDLNKDLLGKISFAQMLYLMLEGRLPSAEQALMID
                     AMLIVLVDHGMTTGAAAARMTFHSAPEAIQGAVAAAILGAGSVHLGSSEHCAKMLNDA
                     LPKDAKETDLEAIALKTVEKRLGNNQLIPGIGHGIHTGGDPRAERLFEIAQETKVYGR
                     YCELLKKIGKIADQKVGKHLPVNVTGAIAAISLDMGIHWQMSKSFAILGRALGGLAHV
                     GEEIRRPIARGISNLIRDNLQYEPEK"
BASE COUNT         1242 a         1546 c         1581 g         1326 t
ORIGIN      
        1 cgcgaaatgg taagctaaca tcgcgttccg attcggtcaa tgcgcgttaa atttcgacaa
       61 ggatagatga tagaggattg cgatgcttca atccggtata tgcacccccg tcctactcaa
      121 tctcattatt tgtagtaacc ttcctttaat agttgatcga caagagtagt atcgacgaac
      181 gatgcggcgt tcgcgctggc agctttgggg gtattttttt tgatcgcttc gagagtcgtt
      241 gtgaccgtat ccacccgcgg ccgcgggatg cgatcggtcc aaagcttgta ctgattatac
      301 gcgtattcgg tttcgtcgcg tggccgtccc aggtattcgg tcatgatctc gatcgtttta
      361 tccttctggt cgaagtaaaa tttctctgcc tcggcggttg cgcgcagcaa acgcagcacg
      421 ttgtcgcgat tgctttttag atacgaagcg gggactaatt cgccgttttg ctgccacgga
      481 atgccgagac cgggaagtgc caagagcatt tcgtagccgc gtctttcggc gatcagatcg
      541 aagggcggcg cgagaatgcc gaactgcact tcgccttttt ccatcgccgc cagaatgctc
      601 gcgggatcag cgttcgagtg ctgcactaac ttgacatctt tttcgggatc gatcttcagg
      661 cgagtcaacg cgaagcgcaa ggcggtatcc ggaatggttc cgaagggagc gcccacatgg
      721 ccggttttgc ccttgagatc ttcgattttt ttgatgcctt tttgcgccat gatcttgtac
      781 ggaaaaacat tgacccagga ggcaactaac acaaacggcg gcgccccttc gagggtcgca
      841 gtgacgaacg ccgcagcgcc cgctccggtg gccatttgca tttttccggc gagcatcgcc
      901 tgcacggcgg cagtggtatt ggtcttgatg atttccacgt cgaggccgtg ctttttgtag
      961 aagccctgat tgaccgcaat cgtgaacaat ccggccccgg gattcacgcc cgcttgcccg
     1021 acgatcatct tgcggctttg cgccttgctc ccggccggga agagaaatgc aattgcggcc
     1081 gatgcgagta gagcgaatgt tgtttttttt acgatgctca ttattcctcc tgacttcccg
     1141 tattgctgtc gtcctgagac gttagtcgaa ggatcccgaa tctgctctag gtggcttaaa
     1201 atacgagatg cttcgccgcg ctcagcatga cagtagcaac tctttcttat cgggggatgt
     1261 taggccaaag ccgcctacga cttcggtggt ttccattttt ccaatcgccg ctcgaaactc
     1321 ttgagtagct cgttcaagcc ggcgcccaag ccggcgatga tgacgatcgg cacaaacatc
     1381 gccgccatct ggaagcgctg cgcggcgacg gagctcaaat aaccgagacc catggctgca
     1441 ccgtattgct cggcgacaac gatgtaaacc agcgcacggc cgagcgcaac gcgcatgccg
     1501 gcgacgatgt agggaagaga accggggagc gagactttgc gaaaaatttg attatctttg
     1561 gcgccgaagg cgcgcgccat cgtgatcagg cgatagtcag tattagcaat tccaaccatg
     1621 gtattgatga gaatcgggaa caccgcggcc aggacggtca taatgatttt cgaaatcgcg
     1681 cccagaccga acagcatgat gatgagcggt aacaacgcga tcagcggtgt cgcgtaaatg
     1741 ccggacaaca acggatcaaa tgttttgctg acgcctttgt accagcccat cggcacgccg
     1801 agaagaacgc caaggacgac ggaaataaca aagccgatgg caaaattcgt caggctgaac
     1861 cacatgtgct cccaaatcgc gccgctggca aaaagctcgt aaccttttgc gagaattttg
     1921 ctgaatggtg gaaaatagaa cggattgaga atttccaggc gggtgaagag ctcccaggca
     1981 agaaaaaaca gcacaattcc aatgacgccg agccagtcgg aaattaactg gcgcgcgcgc
     2041 tgccaggcgc ggtgcgtcgt cagcggattc gactcatcga cggagacgag atcgacaacg
     2101 tctggtttgg attttgccgt aggggccctc cgactaactc cgagggacaa tatgcgtcgc
     2161 caggtcgtac tgtcaaggcg cggcgcgaag gcgacgcgat ttcaaatctc aaattcaaat
     2221 cgacttcgac cttgcgtgca actgaaaaca taatttgcga ttcggagcgc agcgaggact
     2281 actcccagcg tttcatgctg gaaaaatcgt agttcactga cgtgtcagct tcgaagcgcc
     2341 atcgtgcacg atcacgtttc tgctcgccga tgaaaaccat ctgcaaaaat gacgtgattt
     2401 ggctccaggc ggcatcgctc tgcggccggc ggtagcggcc aggcattgtg tcgttcaaaa
     2461 acccatgggg tgcgtcggga tagatgcgga tatcgaagct tttttttgct ccggccaaga
     2521 ccgtgagcat gcgcaggata ttgtctcgat gaatcaaatt atcgagctcg ccgaaaactg
     2581 cggtgagggg acatttaatt tcttgcatta gtttgtcgat cggttcggga cggacccggt
     2641 gcgatttcca gtcggcgtcg taaaccgctc cgtaaaggac gacagcggcg ctgagatagt
     2701 cgcgttcggc tccgaccaaa atgggttgtc ggccggtctg gcatacgccc gacatggcga
     2761 ctttgctcat atcggcctgg ggcacagtgc gtaagtaggc gaccacggcg tccgtgtcga
     2821 ctaagacttg ctcgtcgtcc agctcacagc gatcatcgcc tgccgcaagc ttttggcgat
     2881 cgccggtaaa acgcgaaaaa taatctggca cgatgctcac gtaaccggcg tcgacgaatt
     2941 tttgcccgag atcggttgtg tgctggacga tgccgtagcg ctcatgcaaa tgaatcacga
     3001 ccggtttctt gccgtcgccg gctgggatgc cgagaaatgc attcattcgg ttctcgatct
     3061 tgatgggtgt aagcatgggt acggtccttc ctttgttttt tgatcacctc aggttgaaga
     3121 ttcgaatact tcgccgccgt catgaccgcg aactccgggc gtatgccata cgccctacga
     3181 atggagtccc gactcgggct gcataacccg agttggttgg gcacaaggca atccgttcgc
     3241 gcaattcacc tacggcagct tgctcaggat gtgcctcagc accgctttgg ttgtttccag
     3301 cgacgactcg ggttttctcc aggcgctgac cgcgccgccg gttctgcctt tatagaacac
     3361 aatcaacgct gatttgtagt tcgtcttcac atcggtcact gcttcaacaa taaacgcttt
     3421 gtcgccgaat ccgctgaccg gcgtgatctc agcatttttg aatggcgcgt ccgccgcttt
     3481 ggccatttct cgatattggt cgaaagaacc atcgtagacc gttttcatat cgtcggcgga
     3541 attcaaaaaa tgcagcgtga catcgagaaa ccgactggcg ccgaccggat tctgaaaact
     3601 tcccccttta gcgatgtacg tgcagaccga atcataggtg tcggcgccgc ccgcagtcgg
     3661 gactttggca aactcctgca catcgagctc cccgccgacg agtttctgag catcggcgcc
     3721 gctgacgatg ctgcaagctg tgagttccgg cgcattcgca cccatcgccg aaacagcaag
     3781 aatcagccaa ctgaaggcga cgatcacgta aaaatttatg atcacaatgc ggtccgaacc
     3841 agtctacttt ttgtacaaac gctcaaaata gccttcatca tcgagttttt tcacgaagct
     3901 cgagtcgacg aagtcttcag gacgcgaggc tttcgctttc gggtttttcg gcgctagctc
     3961 gtcgagaatc gtctgaattc ccggcaaggt cgggtaaggc ttttccggca tcgttttgag
     4021 cgcgatctca cgataggtct cttcgagggc ttcacgatcg gtggtctgga gatagcggct
     4081 gaggatcttc agcaccggtt ccttctcacg cttgtaaaaa tgtaccgcgt cagccagcgc
     4141 agcgacgacg tcgagaacga tttcgcgctg tgttttgata taagccttgc gcgttaagag
     4201 cgcggtcagc tcgaacggaa tattcaattg cgccatgtcg atgagcgtgt tgtagccctg
     4261 tttgcgcgcg gtcagcgtga acggcggcgt aatgatcgtc gcttggactt gtttggactg
     4321 caacgcggca aatcgtgtgg tctgcgtgcc gatctggaga atcgttacat ccttgtccgg
     4381 gttcagtccg actttagcca aggacattcg cacgctgacg tcggtcgcgc tgccgaaccg
     4441 gctgatcgcg accttgccgc cgacgagttg ttccacgcgc ttcacttccg gaatcgagat
     4501 caagctgtaa ggaaaggtat tcaagaaact tgcgatgatg accacgtcgg cgccggcaag
     4561 attgctcgtc accgccgcag atccatcggc catgaccatg gcgacatcgc ctcccatcat
     4621 cgcttgaaaa cccagcgagc cgcctggcgt caggataagc tgcgggtcaa ggttgtgctt
     4681 tttaaatatg cctgcctcct tggcaatgat cagtgcggcc tgataggggc tcgtcgccgt
     4741 ataggcgatg ttgattttgc gtggttgagc ggaagcgggc atcgctccgg acaaaatcag
     4801 tagcatggcg aagacgactc tggtttgaaa acacgcgcat attctgttca ccatacctcc
     4861 aatcaccagc tcaggaagaa atgcaggcgg tccggaaagg gcgtatgcca tacgccccta
     4921 catcaaacat acctttacct cacctagcga aaaccagatc tatttctccg gctcgtactg
     4981 caaattgtcg cgaatcaaat tggagatgcc tcgggcgatg ggccggcgga tttcttcgcc
     5041 gacgtgcgcg agacctccga gcgcgcggcc gaggatagcg aagctcttgg acatctgcca
     5101 gtgaatgccc atatccagag agatcgccgc aatcgcgccg gtgacattca ccggcaagtg
     5161 cttgccgact ttttggtccg cgatcttgcc gattttcttg agcagctcgc agtagcgccc
     5221 atagactttg gtttcctgcg caatttcgaa gagccgctcg gcacgggggt ccccgccggt
     5281 gtgaatgccg tggccgatac cgggaataag ctgattgttg cccagccgtt tttccacggt
     5341 cttgagcgcg atcgcttcta aatcggtttc ttttgcatcc ttcggcaacg cgtcgttcag
     5401 catcttcgcg caatgttcgg aggaacctaa atgcacgctg ccggcgccga gtatagcggc
     5461 cgcgaccgcg ccttgaatcg cttccggcgc ggaatgaaag gtcatgcgcg ccgcagccgc
     5521 gcccgtggtc atgccgtgat cgacaagaac gatgagcatg gcatcgatca tgagggcttg
     5581 ctcggcgctc ggtagccggc cttcgagcat gagataaagc atctgcgcga agctaatctt
     5641 gccgagcaga tctttgttca aatcgtaatt gcgcacgaaa atgcgatcga gctcg
//
LOCUS       DASULZ010000011         2206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_812869, whole genome shotgun sequence.
ACCESSION   DASULZ010000011 DASULZ010000000
VERSION     DASULZ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812869"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VFY96_00205"
     CDS             <1..774
                     /locus_tag="VFY96_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5604901.1"
                     /translation="DVDFVSGNHISPYLLVKRGKPIVCLTSPSNTTNDRLVTNFPITR
                     IAELRGKRIGDTTLVDSIGGYHHPRGNHMLYVMRDGLKLDEVEWVELSESNNEFKAMQ
                     MHVLKSKKVDAIFVTGNTDQFRQAGMHVLALDRLPMINGPTLTSTLTTLQNKPGLAER
                     LVKAQVMGIHFARTRRGETEAILDGLRQRVPECKNVQYRSVAKLLPKPYPEHEAVANA
                     YRLCCLKTPETEEQSPMALWDLHYLRLLDHSGFIDKLYA"
     gene            826..1983
                     /locus_tag="VFY96_00210"
     CDS             826..1983
                     /locus_tag="VFY96_00210"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5604902.1"
                     /translation="MVSAVAPAGDNVNASMSILKFITVAIYVVCAASSSHAAQADWRK
                     DWEQTLSAAKKEGQVNIYIYRYEALLQDFKRDFPGINVAAVTGRGNELTNRIMAERRA
                     GKYIVDVYSGGTNSLYNTLYKGKALDPFKSLLVLPEVTDVSKWWGNEHRYADPEGKYI
                     FSFIGSPSNSQLAYNTKLVDPKEFNSYWDVTNPKWKGKVVSLDPRDTGLGATMQFFYY
                     SPEIGPEFMKKFFGAMDITYAKNFRQMTDWLAQGKFAICMGCKDSMRAKNQGLPVDDF
                     DTTKWKEGSSFSAGGGSMAYMNQAPHPNAAKVFINWFLSRKGQIALQKLGDVDDPANS
                     RRIDIPKDDIPPDNRMQPGVKYFDVVKPEYGDMKPIFDLAKEIMASVEKGK"
BASE COUNT          565 a          556 c          613 g          472 t
ORIGIN      
        1 gatgtggatt tcgtatccgg caatcatatt tcaccttatc tgcttgtgaa gcgcggcaag
       61 ccgatcgttt gcctcacctc tccgtcgaac acaaccaacg atcggctggt gacgaatttt
      121 ccgatcacgc gtatcgccga gttgcgcggc aagaggatcg gcgacacgac cctggtggat
      181 tccatcggcg gatatcatca cccgcgcggc aatcacatgc tttacgtaat gcgcgatggg
      241 ctgaaactgg atgaagtaga atgggtagaa ctgtcggagt cgaataacga attcaaggcg
      301 atgcagatgc acgtgctcaa atcgaaaaaa gtcgatgcga tcttcgtgac cggcaacacg
      361 gatcaatttc gacaggccgg catgcatgtg ctggccctcg atcgcttgcc gatgatcaat
      421 gggccgacgc tcacttccac gctgaccacc ttacagaaca aaccaggatt ggcggagcga
      481 ctggtcaaag cccaagtgat gggtatccat ttcgcgcgca ccagacgagg tgaaaccgaa
      541 gcgattctgg atggcttgcg ccagcgagta ccggaatgca aaaacgtgca atatcgtagc
      601 gtcgcaaagc tactgcccaa accttatccg gaacacgaag cggtggccaa tgcttatagg
      661 ctttgttgtt taaagacgcc cgaaaccgaa gagcaaagcc ccatggcttt atgggatttg
      721 cactatttgc gtctgctcga tcactcgggc ttcatcgata agctttatgc ctaaagtatt
      781 ttgcgttgtc agcacattgc catgaaactc agcgattgct attaaatggt ctcggcggtc
      841 gcgcctgccg gcgacaatgt caatgcgagc atgagtatcc tgaaatttat taccgtagcg
      901 atttatgttg tctgtgcggc ttctagcagt catgccgctc aggcggattg gcgaaaggat
      961 tgggagcaga cgctgagcgc cgcaaagaaa gagggacaag tcaatatcta catttaccga
     1021 tacgaagcgt tgctgcagga ttttaaacgg gactttccag ggatcaatgt cgcggcggta
     1081 acggggcgcg gcaacgagct caccaaccga atcatggccg agcggcgcgc cgggaaatac
     1141 atcgttgacg tttacagcgg cgggacgaac agtctgtaca atacgctcta caagggaaaa
     1201 gcgctcgatc cgttcaagtc cttgctggtg ctgcccgaag ttaccgacgt atcgaagtgg
     1261 tggggcaacg agcatcgcta tgccgatcca gaggggaaat atattttttc ttttatcggc
     1321 agcccgagca attcccaact ggcgtacaac accaagctcg tcgatcccaa ggagttcaac
     1381 tcttactggg acgtcaccaa cccgaaatgg aagggcaagg tcgtctctct cgacccgcgt
     1441 gacacgggac tgggcgcgac tatgcagttc ttttattaca gtccggagat cggtccggag
     1501 ttcatgaaga aatttttcgg cgccatggat atcacctacg cgaaaaattt tcgccagatg
     1561 accgactggt tggcacaagg aaaatttgcg atttgcatgg gatgtaagga ctccatgcgc
     1621 gccaagaatc aagggctgcc ggtggacgac ttcgatacga ccaaatggaa agaaggctcg
     1681 agcttttcgg caggcggggg ctcgatggcc tatatgaacc aggcgccgca ccctaacgcg
     1741 gcaaaagtct tcattaattg gtttctgtcg cggaaggggc aaatcgctct gcagaagctc
     1801 ggtgatgtcg atgatccggc gaactcgcga cgcatcgaca ttccgaaaga tgacattccg
     1861 ccggacaaca gaatgcagcc gggggtcaag tatttcgacg tggtgaagcc ggagtacggt
     1921 gatatgaaac cgattttcga tttggcaaaa gaaattatgg catcggtgga gaagggcaaa
     1981 taaggcgttc gagcttgcgc gttcaaggtt caagatattt gccaacaatc ctgaaaatcg
     2041 aactttgaac ctgcaaccgg agaaaactat gctcacacgc gaagaaaacg aaacgctcac
     2101 ccgagtcgga cccggaacgc cgggaggtga actgttgcgg cggtactgga tgcccgtcgc
     2161 atgcgccggt gaactgaccg acgcgaagcc gattaaaccc tttcgc
//
LOCUS       DASULZ010000012         3055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1218717, whole genome shotgun sequence.
ACCESSION   DASULZ010000012 DASULZ010000000
VERSION     DASULZ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218717"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1752)
                     /locus_tag="VFY96_00215"
     CDS             complement(121..1752)
                     /locus_tag="VFY96_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5604903.1"
                     /translation="MAVPTPRQIAKQFTAHYQPIVDLNSGAIAGFEALARVSNPDGSM
                     STAGALIENIERRTDTLKALIQTILSSIRRDMVPLFDRYPHFYVSVNIPPSILGSGQI
                     LPMVEALELTPYLDRIVCEVTERQALTQVGRSALEGARRSGMSVAIDDFGTGHSGLMQ
                     IVGLDFDILKIDHSLIDKILTNQTAARMLRGIVALAAALRLHTVAEGVESQEEAFFLR
                     AAGVDCGQGWFWSKAVSGPAAARLLKQRYPLRGDRRTGRKDLERICTLSPARYTVPFV
                     KSIFHPSDFSKASELAFAHALAIALFRKTELVIMHAGGDRVGDWAQFPPVRRTLERWE
                     VLEPGSPRSAVYEKLAIRVTKVSTGGDPVEASIKHIEKQKPDLVVLATRGRHGLPLWL
                     KPSIAQAIARRSHAMTLFVPRGCRGIVSPDGVIQLRRILLPIDDHPNAQEAAIRAVRA
                     AEAFGDETVEIVILHVNGGALPEFARPESEACSWKELRRKGDVAETILDVANNIDLIV
                     MATEGRQGVVDAMRGSVTERVVRGTPCPVLAVPAA"
     gene            complement(1888..>3055)
                     /locus_tag="VFY96_00220"
     CDS             complement(1888..>3055)
                     /locus_tag="VFY96_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX5604904.1"
                     /translation="NIGGAPALAGLRERLQQRDIKLLLDFVPNHMAPDHPWVDEHPDF
                     FVHGTESDLTRAPRNYCRMHNKTGSVVFAYGRDPYFDGWPDTLQLNYGNPALQEAMIG
                     ELLKIADQCDGVRCDMAMLILPEVFQRTWGIEAELFWPKATQRVREKSPEFMFMAEVY
                     WNLEWTMQQQGFDYAYDKRLYDRLREGHARSVREHFHAGLDYQNKLARFLENHDEPRA
                     AAVFAPEVHQAAAVITFLSPGLRFFHQGQFEGKTKRISPHLIRGPYEPVDERIAQFYD
                     RLLAVLRQPVVRDGQWQLLECIPAWEGNWTSEGFVAFLWRGTSEEKLLVAVNYAAHQS
                     QCYVRLPFAELRDKQWRLEDLLSAAHYDRNRNDLESRGLYLDIAPWGYHAFKLL"
BASE COUNT          695 a          896 c          818 g          646 t
ORIGIN      
        1 aacagcgaag cgccggatcg acagcgtaaa tggaactatt aaaacgagcc gtggcaacag
       61 ctagtcgggc tgtgcggcac tattcgttgc agcgggttga agcagcagct ccgcgtacgt
      121 tcacgcggcg ggcacagcca atactggaca gggcgtgccc cgaacgacgc gctccgttac
      181 gctgccacgc atcgcatcaa ccactccctg acgcccttcg gtcgccatga caatcaggtc
      241 tatattgtta gccacatcga ggattgtttc tgcgacatcg cctttgcgtc gcagttcttt
      301 ccacgaacaa gcctcacttt caggccgagc gaattctggc agtgcgccgc cattcacgtg
      361 aagaatcacg atctcaacag tttcatcgcc gaacgcttcc gcagcgcgga ctgctcgaat
      421 ggccgcttct tgcgcattgg ggtgatcatc gattggcagg agaattcgac gcaactgaat
      481 cacaccgtcc ggcgatacaa tgccgcggca tccgcgcggg acaaacagcg tcatggcatg
      541 acttcggcgc gcgatcgcct gagcgattga cggtttgagc cataacggca aaccgtggcg
      601 tccgcgtgtt gcgagcacga ccaaatccgg tttttgtttc tcaatatgct tgatgctcgc
      661 ttcgaccgga tcacctcccg tgctcacctt cgtgactcgg atcgcaagct tttcataaac
      721 cgcagaacga ggactgccgg gctccagcac ttcccaccgt tccaaagttc ttcgcaccgg
      781 tggaaactgc gcccaatcac ccacgcgatc gcctcccgca tgcatgatca ccaactcggt
      841 cttgcgaaac agcgcgatcg ccagcgcatg agcaaaggcc aactcactgg ccttggaaaa
      901 atccgaaggg tggaaaatcg attttacgaa cggcaccgtg tatcgtgctg gactcaatgt
      961 gcagatcctt tcaagatctt tcctgccggt acggcgatct ccgcgcagcg ggtatctctg
     1021 tttcaagagc cgggctgctg cggggccgga tacagcctta ctccaaaacc agccttggcc
     1081 gcaatcaacg cccgcggcgc gaagaaaaaa agcttcttcc tggctttcca cgccttcggc
     1141 cacggtatgg agccgcagtg cggcggcaag cgctacgatg ccacgcagca ttctagccgc
     1201 cgtctgattg gtaagaattt tatcgatcaa ggagtgatca atttttaaaa tatcaaaatc
     1261 gagcccgacg atttgcatca agccgctgtg gccagtgccg aaatcgtcaa tggcgacgct
     1321 cattcccgac cgcctggccc cctcgagcgc cgatcgtcca acttgcgtca atgcttgccg
     1381 ttccgtcact tcgcagacaa ttcgatccaa atagggcgta agctccagcg cctcgaccat
     1441 cggcaagatt tgaccgctgc ccaaaataga cggaggaatg ttcacgctca cataaaaatg
     1501 cggatagcga tcgaaaagag gaaccatgtc gcgccggatt gatgatagaa tcgtttgaat
     1561 caatgctttg agtgtatccg ttcgccgctc gatattttcg atcagagcgc ctgcggtgct
     1621 catcgacccg tccgggttac tgacccgcgc gagcgcctca aagccggcaa tcgcgccgct
     1681 attcagatcg acgatgggtt gataatgagc cgtaaactgt ttggcgattt gacgaggtgt
     1741 cggaacagcc acaaaactcc tattaccgaa actactttaa ggagaaatag acggtcggac
     1801 agcaaaaccc gtcacaatcg tacttacgcg ctgtgtaacc agccatcgat tcccgtggtc
     1861 cgcacggttc tcggatgacg ggctgcatca cagcagcttg aaagcgtggt aaccccaagg
     1921 agcgatgtcg agatacaggc cgcgcgattc gaggtcgttg cggtttcgat cgtagtgcgc
     1981 agcgctcagc agatcctcga gtcgccactg cttgtcgcgg agctcagcga acggcaaacg
     2041 aacatagcat tggctttgat gggcggcata gttgacggcg acaaggagct tttcttcgct
     2101 agtgccgcgc cagagaaagg ctacaaaccc ttccgatgtc caattccctt cccaggcggg
     2161 aatgcattcg agcaattgcc actggccgtc gcgaacaacc ggttgtcgca gtacggcgag
     2221 cagccggtca tagaattgcg caatccgctc atcgacgggt tcgtatggtc cgcgaatgag
     2281 atggggcgag atacgcttgg ttttgccctc gaattgtccc tgatgaaaaa agcgcaggcc
     2341 cggcgacaga aaagtaataa cagccgcagc ttgatgaact tccggcgcaa acaccgcagc
     2401 tgctcgcggc tcatcgtggt tttccaaaaa gcgggcaagt ttgttttgat aatccaatcc
     2461 ggcgtgaaaa tgttcgcgca cggagcgggc gtgaccctcg cgcagacggt catacaggcg
     2521 cttgtcgtag gcgtagtcaa agccttgctg ctgcatcgtc cattccaagt tccagtagac
     2581 ctccgccatg aacatgaact caggcgattt ctctcttacg cgctgcgtcg ctttcggcca
     2641 gaacagctcc gcctcgatgc cccaagtccg ttgaaatacc tccggcaaaa tcaacatcgc
     2701 catgtcgcag cgcacgccgt cacattgatc ggcgattttc aatagttcgc cgatcatcgc
     2761 ttcctgcaat gccggattgc cgtagttgag ttgaagagta tcaggccaac catcgaagta
     2821 ggggtcccgg ccataggcga ataccaccga cccggttttg ttgtgcatgc ggcaataatt
     2881 tcgcggtgcg cgagtcagat ccgactcggt gccatggaca aaaaaatccg ggtgctcgtc
     2941 cacccatggg tgatccggcg ccatgtgatt cggcacaaag tcgagcaaca gcttgatgtc
     3001 ccgttgttgc agccgctctc gcagacctgc gagcgctgga gcgccgccga tattt
//
LOCUS       DASULZ010000013         2598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1117328, whole genome shotgun sequence.
ACCESSION   DASULZ010000013 DASULZ010000000
VERSION     DASULZ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117328"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1163)
                     /locus_tag="VFY96_00225"
     CDS             complement(57..1163)
                     /locus_tag="VFY96_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX5604905.1"
                     /translation="MSKIFNVIIAGGKGTRFWPLSRAHRPKQILKLLSRNSLIRETAE
                     RVYPLAGRKQTLVVTIADQLSLLRRELPGVPKNNFIAEPQGKNTAPCIGLAALEVMRR
                     DPEAVMVVLPADHWVADIGAFRKTLKTAVKLAAETDQLITIGIKPDYPETGYGYIMKT
                     RPIGAKSNAAFVVKRFVEKPTLSAARRLIRQHGLWNSGIFIWKASTLLTALRRYQPEI
                     YQSLEKINKAAAAHSLTSWAPRLRKIIADVYSGMANLSIDYALMEKAGAEGKVLTLEG
                     QFGWSDVGSWAAVHRMMPKDRNGNASQNRWLGVDTNNCLIHAPNRLVVLLGVESTVIV
                     DTPDALLVADLQRSQEVREVVDALNREHLNSYTT"
     gene            1211..2176
                     /locus_tag="VFY96_00230"
     CDS             1211..2176
                     /locus_tag="VFY96_00230"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX5604906.1"
                     /translation="MTDQGTMDATKAWNVAPEHEQLRVDAFLRHCLPQLSRRQLASAI
                     ADRLVSLNGRVSKKGDRVSVGDVLVFHGPAAWLSPQTPANPKLQLRVVYEDSELLIID
                     KPAAMPTHGFSGRDQHTVANFIAAKHPELLAIGKSRWEPGILNRLDRDTSGLVLVAKT
                     QRSFIALRKQFRHREIVKTYWALVWGKPSASGTITLPLAHDPRDKTRMRTVDPAHLQT
                     ERIWNASTHYRKLAQRIGVALLELQMMTGVTHQIRVHLAAIGYPIVGDLLYGAVNPES
                     FGLNRQFLHAIKVQFRHPKTGRKLTVEAPLPDDLMKVLTRLKLSF"
     gene            complement(2173..>2598)
                     /locus_tag="VFY96_00235"
     CDS             complement(2173..>2598)
                     /locus_tag="VFY96_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5604907.1"
                     /translation="KISLSNLSESGASIQGALNKTKMPRAKKKSASRAGKDRTHEYID
                     DGTHDESEDEDLPSDEAGRRKIKKNYEELLGIRVEKLRGRVLNREKINKSIDGLYFIC
                     FECKRICHNLDDGWVEEAATLNNYCGNCAKLKAIRSSAA"
BASE COUNT          586 a          689 c          689 g          634 t
ORIGIN      
        1 aagcgggcca tttggagaga gatgcttcac cgaagaacaa tcccgagatg taagacttag
       61 gtggtatatg aattcaagtg ttcacgattc aacgcatcca cgacttcgcg aacttcttga
      121 gagcgctgga gatccgcgac cagcaacgca tccggggtat cgacaatcac ggtactctca
      181 acgcccagta aaacgaccaa ccgattcgga gcatgaatga gacaattgtt cgtatcgact
      241 ccaagccagc gattttgact ggcgttgccg tttcgatcct tcggcatcat ccgatgcacc
      301 gctgcccaac tgccgacatc gctccagcca aattgacctt cgagagttaa aaccttgcct
      361 tcagcgccgg ctttttccat gagagcataa tcgatcgaca ggttcgccat cccactatag
      421 acgtcggcaa tgatcttacg cagccgagga gcccagctcg tgagcgagtg agccgcagca
      481 gctttattga ttttttccaa tgactgataa atttccggtt ggtaacggcg caatgcagtg
      541 agcagcgtcg atgccttcca aatgaagatc ccgctgttcc acaacccatg ctgccggatg
      601 agtcgccgtg ccgccgacaa ggttggcttc tcgacaaagc gttttacgac aaacgccgcg
      661 ttcgatttgg ctcctatggg cctggttttc atgatgtagc cgtaaccggt ttcagggtaa
      721 tcgggcttaa ttccgatcgt gatcaactga tcggtttcag cggccagctt gacggccgtt
      781 ttaagcgttt ttcgaaacgc accgatatcg gcgacccagt ggtcggcggg aagtaccacc
      841 atcacggctt cgggatcgcg gcgcatgact tccaaagccg ctaggccgat gcacggagcg
      901 gtattcttgc cctgcggctc ggcgataaag ttatttttcg gtacaccagg caattcgcgc
      961 cggagcaacg aaagttgatc ggcgatggtg acgaccaacg tttgcttccg acccgccaac
     1021 ggatagacgc gttcagcagt ttcccggatc aagctgtttc gactaagaag cttgagtatt
     1081 tgcttgggtc ggtgggcacg gctcaatggc caaaaccgcg tccctttccc gcccgcgatg
     1141 atcacgttga aaattttact cactggatcg ccttattgat gagatcgcgg ggtatagtac
     1201 ggtagtcgat atgacggatc aaggcacgat ggacgcgacc aaggcatgga atgtcgcgcc
     1261 cgagcacgag cagcttcgtg tggatgcttt tctacgccat tgcctgccgc agctttcccg
     1321 ccggcaactg gcgagcgcca ttgccgatcg actggtttct ctcaacggca gagtaagtaa
     1381 aaaaggcgac cgtgtgagcg tgggtgatgt cctagtgttt cacgggccgg cggcatggct
     1441 gagtccgcag acgccggcga atcctaagct gcaattacga gttgtgtatg aagactcaga
     1501 actcctgatc attgataagc cagccgctat gccgacgcat ggcttctcgg ggcgggatca
     1561 acacacggtg gctaacttca tcgctgcaaa gcatcccgag ttgctggcga tcggcaaatc
     1621 tcgttgggaa ccgggaattt taaaccggct tgatcgcgat acctctggac tggtgctggt
     1681 ggctaaaacg caaagaagct ttattgcgtt gcgcaaacag tttcgccacc gggagatcgt
     1741 taaaacttac tgggcgcttg tgtggggaaa accaagcgca agcggcacca tcacgctgcc
     1801 cctagcacat gatccacgag acaaaactcg gatgcgcact gtcgatccgg cgcatctgca
     1861 aacggagagg atttggaacg cttcgactca ctatcggaaa ttggcgcaaa ggatcggagt
     1921 tgctttgctc gagctacaga tgatgaccgg agtgacccac cagattcgcg ttcatctagc
     1981 ggcgatcggc tatccgattg tcggcgacct gttatatggt gcggtaaacc cggagagttt
     2041 cggtcttaac cgtcaatttc tgcacgcgat caaagtgcag ttccggcatc caaaaaccgg
     2101 ccggaaattg accgtcgaag cacctttgcc ggatgatcta atgaaggttt tgacgcggtt
     2161 aaaattgtcg ttttaggcag cactgctgcg aatggcttta agcttcgcgc agttaccgca
     2221 ataattgttg agcgtcgccg cctcctcaac ccatccatca tccaaattgt ggcagattcg
     2281 tttgcactcg aaacagataa aataaaggcc gtcgatgctc ttgttgattt tttcgcggtt
     2341 aaggacgcgc cctctaagct tctcgacgcg aatacccagg agttcttcat agtttttctt
     2401 tattttgcgc cgacccgctt cgtcacttgg aagatcctca tcttccgatt cgtcgtgagt
     2461 gccgtcatcg atatactcat gggttcgatc tttgccggcg cggctggcgc tctttttttt
     2521 cgctcgtggc atcttggttt tgtttagcgc tccttgaatg ctagctccgg attctgaaag
     2581 attgctcaaa gatatttt
//
LOCUS       DASULZ010000014         2409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1153, whole genome shotgun sequence.
ACCESSION   DASULZ010000014 DASULZ010000000
VERSION     DASULZ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..449)
                     /locus_tag="VFY96_00240"
     CDS             complement(99..449)
                     /locus_tag="VFY96_00240"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEX5604908.1"
                     /translation="MKSETHRLGDNPNFDEHADEDSYFAVKEHELIENMKHEFRKDET
                     TRRVAQMETCPKCSGKFEKDHILGVVVDRCEACEGIWLNKGELQGILRQQARGPLGVF
                     LDRCFSKVKNGNNP"
     gene            complement(512..2404)
                     /gene="ftsH"
                     /locus_tag="VFY96_00245"
     CDS             complement(512..2404)
                     /gene="ftsH"
                     /locus_tag="VFY96_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX5604909.1"
                     /translation="MIFLVLSMQNFFAGTHVELINYSQFKALLKKDLISDLVIRETTI
                     DGNLKGAAVKESFTPERLKEVSPDVLAGKKVFPFKTVRVEDPTLTSELETAKLPFKGE
                     VSNNWLPTILSWVVPVVLFVLMWSYLGRKMGSGSGGLMQIGKSKARVYIEKKTGVTFA
                     DVAGIDEAQEEVAEVVGFLKDPDKYQRLGGHIPKGVLIVGPPGTGKTLLARAVAGEAG
                     VPFFSLTGSDFVEMFVGVGAARVRDLFMQAMKNAPSIIFIDELDAVGKARGVSMLTGN
                     DEREQTLNQLLAEMDGFDPNQGVIIMAATNRPEILDPALLRPGRFDRQILVDRPDIKG
                     RTKILQLHAKKVKLSADLDLALVAAKTPGFVGADLANIVNEAALLAARQDKASIDMAE
                     FDEAIERVVAGLQKKSHVINPKEKKTVAYHESGHALLAELVPGADPVSKISIIPRGIA
                     ALGYTTQLPTEDHYLLTRSELVARIDVLLGGRVAEEIVFGEISTGAQNDLQRATEIAR
                     TMVTQFGMSEKLGLATLEGPRRASFLMVPTESRQEYSEDTARLIDAEVKQILSEAHTK
                     AHDILLAHRAALEDLAQLLLDKEVVDRPALQVILKVRSIDSMKEKKRPPELSGHESNQ
                     TNAERE"
BASE COUNT          493 a          684 c          616 g          616 t
ORIGIN      
        1 tgtgagcctg gtaaaaactt cgcgaacttc ggggaccaga gttcaaattc cagcattcgg
       61 ggtcctcgca gtccctcagc ttgccccggc tacccgagct aaggattatt tccgtttttg
      121 accttggaaa aacatcgatc cagaaagact cccagcgggc cgcgggcctg ttgtctcaag
      181 atgccttgga gttcgccttt gttgagccag atcccttcac aagcttcaca ccgatctacc
      241 acgactccta agatgtggtc tttttcaaat tttcctgaac attttggaca ggtctccatc
      301 tgtgccactc ttcgggttgt ctcgtccttg cgaaattcgt gcttcatatt ttcgatgagt
      361 tcatgctctt ttacggcaaa atagctatct tcatcggcgt gctcatcaaa gttcggattg
      421 tcaccgaggc gatgcgtttc acttttcatg ttttgctccc taatttgttg aatcactacg
      481 gctcatcgcc tactcagccg accagccatg gctattcccg ctcggcattg gtctgattgc
      541 tttcgtgacc ggatagctcc ggagggcgct ttttttcttt catgctgtcg atgcttcgaa
      601 cttttaggat cacctgcagg gccggcctgt cgacaacttc tttgtccagc agcagctgtg
      661 ccaagtcctc cagcgcggcg cggtgcgcaa gcaaaatgtc gtgggccttg gtatgcgctt
      721 ccgatagtat ttgtttgacc tccgcgtcga tcaggcgggc ggtatcctcg ctgtactctt
      781 ggcgactttc agtgggcacc atgagaaacg aggcgcgccg cggtccttcg agagtagcca
      841 ggccgagctt ttcgctcata ccgaattggg tgaccatcgt ccgggcaatt tcggtcgctc
      901 gttgcagatc gttctgcgcg ccggtcgata tttcgccgaa gacgatctcc tccgcgactc
      961 tcccgcccaa aagaacatcg atgcgcgcga ccagttcaga acgtgtcagg agatagtggt
     1021 cttcggtcgg taactgcgtc gtataaccca aggcggcgat tccgcgcgga ataatagaaa
     1081 tcttggaaac ggggtcagcg cccggcacca gctcggccaa gagcgcgtgg cccgactcgt
     1141 ggtaggcaac agtttttttc tccttgggat tgatgacgtg gcttttcttt tgtaatcccg
     1201 cgactacccg ttcgatcgcc tcgtcaaatt ccgccatatc gatcgacgct ttgtcctgac
     1261 gcgccgccag taacgctgct tcattgacga tgttggcgag atcggcaccg acaaagccgg
     1321 gagttttggc tgcgactaat gcgagatcga gatcagcgga aagctttacc ttctttgcgt
     1381 gcaattggag gattttcgtg cgtcctttga tgtcgggacg atccaccagg atctgccgat
     1441 cgaagcgccc cggacggagc agcgcggggt cgaggatctc cggccggttg gtggcggcca
     1501 tgatgatcac accttgattt ggatcgaacc catccatctc ggctaaaagt tgattcagcg
     1561 tttgttcccg ctcgtcgttg cccgtgagca tgcttacccc gcgagctttg ccgacagcat
     1621 cgagctcatc gatgaagatg atgctggggg cattcttcat cgcttgcata aaaaggtccc
     1681 tgacccgcgc tgcgccgacg ccgacgaaca tctcgacgaa gtcggaaccg gtcaggctga
     1741 agaaaggaac cccagcctcg cccgctacgg cgcgagccag aagcgttttt cctgtgcccg
     1801 gcggccccac gatcaacaca cccttcggaa tatggccgcc cagtcgttga tatttatccg
     1861 gatctttcag gaacccgacc acctccgcga cttcttcttg ggcctcgtcg atgccggcaa
     1921 cgtcggcaaa ggtaactccg gtttttttct cgatgtagac tctggctttg ctcttgccga
     1981 tctgcatcaa accgccgctt cccgacccca ttttcctacc aagatagctc cacatgagaa
     2041 caaatagcac cacgggaacc acccaggata aaatcgtcgg cagccaattg ttgcttactt
     2101 ctcccttaaa gggcagtttc gctgtctcta gctcagacgt gagagtcggg tcctccactc
     2161 tcacggtttt gaatggaaaa acttttttac cggcaaggac gtccggagag acttccttaa
     2221 gtctctccgg cgtaaaactt tccttaaccg cggctccttt taaattgccg tcgatggtcg
     2281 tttcgcgaat gacaagatca ctgatcaaat ctttcttgag cagagccttg aattggctat
     2341 agttgataag ctcgacgtga gtgccggcaa aaaaattttg catcgagagc acgagaaaaa
     2401 tgatggcaa
//
LOCUS       DASULZ010000015         4312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1249, whole genome shotgun sequence.
ACCESSION   DASULZ010000015 DASULZ010000000
VERSION     DASULZ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VFY96_00250"
     CDS             <1..817
                     /locus_tag="VFY96_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5604910.1"
                     /translation="PTRLEPRRGTPLSLVRVLGSVAGGSCLSPLLAELFYLRHDCAVI
                     WHNLKSLARAEELSDQLEHEPRWPALVAVLAVGGLYTGLPDALTMGPRWLFPSVVVAL
                     LIPTVVSHHTSRHRLNNSFGFAVDAVLTLGLTISIVLLIAALPTGEESPQALLLSAAS
                     LWITNILVFALWYWRLDAGGPYQRDKTPGHSDGAFLFPQMTMSEQALRDAGIENWSPE
                     FIDYLFLAFNTSTAFSPTDVPVLARWAKFLMMLQSLLSLLIVALLAARAVNIL"
     gene            931..1335
                     /locus_tag="VFY96_00255"
     CDS             931..1335
                     /locus_tag="VFY96_00255"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX5604911.1"
                     /translation="MAIPGEKAIQMVRSAHSQAAQLDIAVTAVVVDQSGRMIALGRMD
                     KARPITVEIALNKAYTAASFQQPTKDLASVAGQSWFQSLVVSSNGRIMPGGGAVPIVE
                     GGSVVGAIAVSGGTDEQDQKCAEVAMSGYTNA"
     gene            1415..2728
                     /locus_tag="VFY96_00260"
     CDS             1415..2728
                     /locus_tag="VFY96_00260"
                     /inference="COORDINATES: protein motif:HMM:NF024877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reductive dehalogenase domain-containing
                     protein"
                     /protein_id="HEX5604912.1"
                     /translation="MSKAEKWDQLPNSTTNDEFPTPYLGRSVRFVGSHVNVDMNQSPH
                     PKNQRGELGKPLFNWYRNTVSKDPLTRVSAPNHFADRRHFLVNVINRSTNGKINPRRI
                     AVDDPAAMARHIKAVALYMGADVVGIAKAHPSFLYAAGGGRYVQDGTAKDDYQSHSPE
                     DLARRFPFIIQATTAWDYNKLQAHRHHIGDAAYHVSQMKGVMVLKALEGYIKELGYTA
                     LRGVVTPQAAGVAGGIGELGRNGLVINKNFGARIHMPDPILTDLPLLADEPADIGVED
                     FCKICRKCANTCPTNSIPFGDKVTFNGIEKYKINWLTCYKLRPYVHDHWGSCLTCAAV
                     CPFTKPNVWWHNLATWALHTCPYSARPALVGALKWIDDKFWGVTRSSRVRWLGYDSGI
                     KPGEQACTVAGCTASHEGKASSVSGEMGYYAPLKENTNRFVKRDG"
     gene            2728..3351
                     /locus_tag="VFY96_00265"
     CDS             2728..3351
                     /locus_tag="VFY96_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5604913.1"
                     /translation="MALRLGPFKFPWFGKDSHKNDPYWDFFINTPPADLANTVIEMIR
                     KAPDGSVFPTKAELHTPEITSSHVKGMAEYFGAELVGIAKLSRNRDLSPLPRGEGNDE
                     EDNVETYPFAIICAVHADYDPRTSHGIGGQAPVQNGLFITFVLSAWIRELGFRASTAP
                     DPDAEKLAAAAGLGTLNSGGRLVTAKFGANVHIANVIRTDLPLAADG"
     gene            3455..4120
                     /locus_tag="VFY96_00270"
     CDS             3455..4120
                     /locus_tag="VFY96_00270"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX5604914.1"
                     /translation="MPYVITSLCTNDGACVEVCPVACIHTTPGAPQFYIDPEVCIDCE
                     QCEIVCPVDAIFKDVDIPPEHTVSIEVNAAFFRQNKAAIGPVPFDMAWEMIWSAHEYA
                     QMVGLAVSAVVVDEAGAPIAVGRMDKAEPNSVELAFNKAYTASAFHMATTELAPQSGA
                     PWMRSLMISHHGRMLPMGGAVPILNGVTIVGAIGVAGASRPEQDVLCCRAAMAVWETP
                     GHH"
BASE COUNT          924 a         1233 c         1221 g          934 t
ORIGIN      
        1 accgactcga ctcgagccac gacgcggcac gccactgtca ttggtacgcg tccttggttc
       61 ggtcgccggg ggctcttgcc tctctccgct actagccgaa ttgttttatc tgcggcatga
      121 ttgtgccgtg atttggcaca atctcaagtc gctggctcgc gccgaagagc tttcggatca
      181 gctagagcat gaacctcgct ggccggctct cgttgcggtg cttgcggtag gcggacttta
      241 caccggcttg ccggacgcgc tcaccatggg accgcgctgg ctgtttccct cagtcgtggt
      301 ggccctgctc attcccactg ttgtttctca tcatacgagt agacatcggc tgaataacag
      361 ttttggcttt gccgtcgatg cggtgctcac cctcggactc acgatctcga tcgtgctcct
      421 catcgcggcg ctgccaacgg gagaggaatc tccccaggcg cttttacttt ctgccgcatc
      481 cctatggatc accaacattt tggtttttgc cttatggtat tggcgcctgg atgccggcgg
      541 gccgtaccag cgcgacaaga cgccgggcca cagcgacggc gcctttctct ttccgcagat
      601 gacgatgagc gagcaagctc tccgcgatgc cgggatcgaa aactggtctc ccgagttcat
      661 cgattatttg ttcctagcgt tcaataccag cacggccttc tcgcccaccg atgtgcctgt
      721 cctggcccgc tgggcgaaat ttttgatgat gttacaatcg ttgttgtcgc ttttgatcgt
      781 cgcgctgttg gccgcccggg ccgtcaatat tctctaaatc gttttgagcc tgcctcggcg
      841 atgtgcttga ctgcctgggc agggtctctg ctaaagtggc aaaatggagt ttggacggta
      901 ctaaccctaa ccaggaggac cccaggcagc atggccatcc caggagaaaa ggcgattcaa
      961 atggtgcgca gcgcacacag ccaggcagca cagctcgata tagccgtaac tgccgtcgtc
     1021 gtcgaccaaa gcggtcgcat gatcgcgctt gggcgcatgg acaaggcccg tccgatcacc
     1081 gtggaaatcg ctctcaataa agcatatacg gcggcgagct ttcagcagcc gacgaaagat
     1141 ctggcaagcg tcgccggaca gagctggttt caaagcctgg tggtgtcgag caacggccgg
     1201 atcatgcccg gtggtggcgc cgtaccgatc gttgaaggtg gcagtgttgt cggcgccata
     1261 gcggtgtcgg gcggaaccga tgaacaagat cagaaatgcg ccgaagtggc gatgtcagga
     1321 tacaccaacg cataaaagta taatggaacc tcgcttggtt tgcctgaaat tcagcccttc
     1381 gccttcttcg tttaaatgat taaaggagcg aaacatgtct aaggcagaaa aatgggatca
     1441 attgccaaac agcaccacca atgatgagtt tcccacgcct tacctgggcc gatcggtaag
     1501 attcgttgga tcccatgtca atgttgacat gaaccagtcg ccccatccga aaaatcagcg
     1561 cggcgaactg gggaaaccgc ttttcaactg gtatcgaaac acggtgtcga aagatcctct
     1621 gacccgcgtc tctgcgccga accattttgc cgatcgcagg cattttctgg tcaacgtcat
     1681 caaccgctcg acaaacggca agatcaatcc tcgccggatc gctgtcgacg atccggcggc
     1741 tatggcgcga cacatcaaag ccgtggcgct ctatatgggc gccgacgtgg tcggtattgc
     1801 caaggcgcat ccgtcgtttc tctatgccgc gggtggagga cgttacgtcc aggacggcac
     1861 cgccaaagat gattaccaga gccattcgcc agaagacctg gcgcgtcgct ttccttttat
     1921 tatccaggcg accaccgctt gggattacaa caagctgcag gcgcatcggc accatatcgg
     1981 tgacgccgca tatcatgttt cacagatgaa gggcgtcatg gtgctcaagg cgctcgaagg
     2041 ctacatcaaa gagctgggtt ataccgcgct gcgcggtgtc gtcacaccgc aagcggcggg
     2101 agttgccggc ggcatcggcg agttgggcag aaacggcctg gtgatcaata aaaatttcgg
     2161 cgcccgcatt cacatgcccg atccgattct caccgatcta ccgctgctgg cggacgagcc
     2221 ggcggacatc ggcgttgagg atttctgtaa aatttgccgg aaatgcgcca acacctgccc
     2281 gaccaacagt attccgttcg gcgacaaggt gactttcaac ggcattgaaa aatacaaaat
     2341 caactggctc acctgctaca agctgcgtcc ctatgtccat gaccattggg gtagctgcct
     2401 cacttgcgca gcggtttgcc cgttcaccaa acccaatgtc tggtggcaca atctggcgac
     2461 ctgggcgcta cacacctgtc cctattccgc gcggcccgct ttggtcggag cactgaagtg
     2521 gatcgacgat aaattctggg gcgtgacgcg gagcagtcgc gtgcgctggc tgggatacga
     2581 ttccgggatc aagcccggtg agcaagcatg tacggttgcg ggatgcacgg catcccacga
     2641 aggcaaagcc agctcggtga gcggcgaaat gggctactat gcgccgctga aagaaaacac
     2701 caatcgcttt gtcaaacggg acggctagtg gcgttgcgac tcggtccctt taaatttccc
     2761 tggttcggaa aagactctca caaaaacgat ccctactggg atttttttat caatacgccg
     2821 cccgccgatc tcgccaacac ggtcatcgaa atgatccgca aggcgcccga cggcagcgtg
     2881 tttccgacca aagcggaact gcacaccccc gagatcacgt cgagccatgt caagggcatg
     2941 gcggaatatt tcggcgccga gctcgtcggg atagcgaaac tatcacgcaa ccgagatctt
     3001 tcgcctctgc cacggggaga ggggaatgat gaggaggata atgtcgagac ttatcccttt
     3061 gcaattatct gcgccgtcca tgccgattac gatccacgca cttcccacgg catcggcggc
     3121 caggcgccgg tgcagaacgg attgtttatc acgtttgttt tgagcgcctg gatcagggaa
     3181 ttgggttttc gcgccagtac cgctccggat ccagatgcgg agaagctggc ggcggcagcc
     3241 ggcctcggaa cgctcaattc aggcggaaga ttagtcacgg cgaaattcgg cgccaacgtt
     3301 cacattgcca atgtgatacg caccgatttg ccgctcgccg cagacggtta acacagttca
     3361 aaccgctgca accgttcaag acgttcaatg tgttttgaac gatttgaact gctcgaaccg
     3421 attgaacgac ttgaacgcat tggagacatt ctgaatgccg tacgtgatca cttccttatg
     3481 taccaacgac ggcgcctgcg tggaggtctg tccagtcgcc tgcattcata cgacaccggg
     3541 cgcgccgcag ttctacatcg atcctgaagt atgcatcgac tgcgagcagt gcgaaatcgt
     3601 ttgtccggtg gacgcgatct tcaaagatgt cgacattccg cccgagcata cggtatcgat
     3661 cgaggtcaat gccgctttct ttcgccaaaa caaagcagcg atcggtccgg tgccattcga
     3721 catggcatgg gaaatgattt ggtcggcgca tgaatacgcg cagatggtag gcctcgcggt
     3781 gagcgctgtg gtcgtggatg aagcgggcgc gccgatcgcg gttggacgca tggataaagc
     3841 ggagccgaat tcagtagagc tcgctttcaa caaggcttac actgcgtcgg cttttcacat
     3901 ggccaccacc gagctcgcgc cgcagtcagg cgcaccctgg atgcgtagtc tcatgatttc
     3961 acatcacggc cgcatgctgc cgatgggagg tgcggtgccg atcctaaacg gcgtcacgat
     4021 tgtcggtgcc atcggagttg ccggagccag ccggccggaa caggacgtgc tctgctgccg
     4081 tgcggccatg gcggtctggg aaaccccagg ccatcactag tgaatcgaaa ggggtcggga
     4141 gtcttaggtc agaacttccg ggcctcttct tacgtccgga accggttcta aaccgatcat
     4201 cctgagtagg cggcgcgcag cgctgccgta tcgaaggaca cgtctatttg acttcccccg
     4261 gaaataactt cgaagcatac tctcggttca ccgcctgaac gagcgtatta tc
//
LOCUS       DASULZ010000016         5573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1524, whole genome shotgun sequence.
ACCESSION   DASULZ010000016 DASULZ010000000
VERSION     DASULZ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VFY96_00275"
     CDS             complement(<1..543)
                     /locus_tag="VFY96_00275"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5604915.1"
                     /translation="MALFFHSDDVDQLISFMEAVQITESALRDLVSPRGVNAPRKRLN
                     LHRDIAEGSFDTVLNVYAGGSASYGAIGAQIALHRKAIVASTQKRPPFNPDQTELALI
                     YDSDTGSLLGVMVHRPRDFTGVADLRTPAASLAGLDLFARKDARRVGIYGSGQQAWAT
                     FAGLCRLRTIEWAKIFSPTPA"
     gene            complement(554..1555)
                     /locus_tag="VFY96_00280"
     CDS             complement(554..1555)
                     /locus_tag="VFY96_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5604916.1"
                     /translation="MQYRTIGKTGLKVSEIGFGCGNNAVLMVRAPYQDQLNAVRHALD
                     CGINFFDTAYAYGLGKSEENLGRILKQLGVAPVISTKIRLEPDCLSDIKGATIQAVES
                     GLSRLGRERVDFVQLHTRVTTARAMGERFSLSPGDVLGANGVIAGFKTMRDRGKVGFF
                     GFSGLGDPDALLELVDSGEFHGFQCYYNLLNPSAGNPVPSSFSALDYRRILDRAAANG
                     MGAFVIRVLAAGALTADPSTGGGGSGQTLSPGSDYSVDLKRAEKVKAALGVGGKELTQ
                     AAIRFALINPNVSTVLVGFSKTSHIDEAVACSGAPGFAKDQMLNMQKLWQSDFSLTE"
     gene            complement(1723..2415)
                     /locus_tag="VFY96_00285"
     CDS             complement(1723..2415)
                     /locus_tag="VFY96_00285"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX5604917.1"
                     /translation="MERLILFDIDGTLTRTENGYVPFNEAILQTFGCTGDIRTVVPDG
                     NTDPLIVQDIFAKSNFVCEFQDTIWQQFTINLRERYHHHVMQGTTKIRALPGTDALLR
                     ALAANEKICPSVVTGNFEATAEIKLETAGLAPYLCRGAYASDSHVRADLPAIAKARCE
                     HITGSALLAEQCIIIGDTSNDLQAARQNHMKCILVGTGRYPIEELRYWEPDACLADLV
                     DTDAVLEILEKI"
     regulatory      2630..2714
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            2836..3978
                     /locus_tag="VFY96_00290"
     CDS             2836..3978
                     /locus_tag="VFY96_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX5604918.1"
                     /translation="MIKHYLLSPGPTPIPNEVQLAMAQTMIHHRTPQFYQVFNETRAG
                     LKKIFGTQNDVLMLASSGTGAMEASVANLFSPGDKVLVINGGKFGERWLNISNAYGLN
                     PIELKVEWGQAVKVADVERQLKQHSDIQGVMIQASETSTTVFHPVKEIAKLTENGPLF
                     LVDGVTAVGVVPLQMDEWGFDVLVTGSQKALMLPPGLGFIALSERAWEKTKRAKLPRF
                     YFDLNLERKNQQKGSGAFTPAVSLIFGARASMNLLEREGLANVYARHARLSRATRAAA
                     TALGLRLLAPTDPSPAATGIYLPEGIDADQVLDYLRDKMNITLAEGQDQLKGKVIRIA
                     HVGYMGSFDVITAIAALEMALRKFGAEIPFGRGVAAAEEVLMEALQ"
     gene            4049..>5573
                     /gene="serA"
                     /locus_tag="VFY96_00295"
     CDS             4049..>5573
                     /gene="serA"
                     /locus_tag="VFY96_00295"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX5604919.1"
                     /translation="MKILVTDPLAPEGLEVFKNADGFEVDVKIGLKPDELKKICHAYD
                     GWVIRSGTKITADLIEAATNLKVIGRAGVGYENIDADTATKKGIVVMNTPGGNNVTTG
                     EHTIALMMALARHIPQAVASLKGGKWDRNKFVGVEMCNKTIGVIGLGNVGRIVAERAA
                     ALRMKVIGFDPFIAAENIARMGVQPRTLDDIFTKSDFITVHVPLTPDTQGLINKAAFA
                     KMKRGVRIINCARGGIVDETDLAEAIKQGKVAGAALDVYVDEPPPPDHPLLKLDQVIT
                     TPHLGASTDEAQLNVAIAVAEQMVDFLAKGVVRYAVNVPSVSPELLQVLRPYLTLAEK
                     LGSLHAQMAGALPEEVQVEYRGDVTQYNVAPLTLAVLKGLLSPVMEFAVNYVNAPILA
                     RERGIKIIESKGEEATDFTSSIIVRAKNGKESLEVEGAIFAAKHPRIVRVNSFYLEAV
                     PEGYILVLQNKDVPGVVGAVGTILGNHGLNIAGMELGRSEKGGNAISFTHVDEAVSKK
                     "
BASE COUNT         1268 a         1565 c         1526 g         1214 t
ORIGIN      
        1 agccggagtg ggactgaaga ttttcgccca ttcgatcgtg cgcagccggc agagccctgc
       61 gaaagttgcc caagcctgct ggccagaccc gtagataccg acgcgccgcg catccttgcg
      121 ggcaaacaaa tccaagccgg ccaagctcgc tgccggcgtg cgcaaatcag cgaccccggt
      181 gaaatcacgc ggccgatgca ccatgacgcc gagcaaagat ccggtatcgg aatcataaat
      241 cagcgccaat tcggtttgat cgggattgaa cggcggccgt ttctgagtgc tggcaacgat
      301 cgcttttcga tgcagcgcga tctgcgcgcc gattgcgcca tagctcgccg agccgccggc
      361 atagacattc aacaccgtat caaagctacc ttcagcgata tcgcggtgca ggttaagccg
      421 cttgcgcggc gcgttgacac cgcgaggcga gaccaaatcg cgcagcgcac tctcggtgat
      481 ttggactgct tccatgaacg agatcagttg atcgacatcg tcggaatgga agaaaagcgc
      541 catgcgaagt tccttattcg gtcagactaa aatccgattg ccaaagtttt tgcatgttca
      601 gcatctgatc tttagcaaag cccggcgctc ccgaacaggc gaccgcctca tcgatgtgac
      661 ttgttttgga aaacccgacg agcaccgtcg agacgttggg attgattaac gcaaatcgga
      721 tcgccgcttg agtcaactcc ttgcccccca ctccgagcgc cgctttcacc ttttccgcgc
      781 gtttgagatc gaccgagtag tccgatcccg gcgataaggt ttgtccgctt cccccacctc
      841 cggtgctggg atccgctgtg agagcgcctg ccgccagaac tcggatgaca aaagctccca
      901 tgccattggc tgcggcgcga tcgagaatgc ggcgatagtc taacgcgctg aacgacgacg
      961 gcactgggtt tccggcgctg ggattgagca aattgtagta gcactgaaag ccgtggaact
     1021 cgccgctatc caccaactca agcagtgcgt caggatcgcc gaggccgcta aagccaaaaa
     1081 agccgacttt gccccgatca cgcatcgttt tgaacccagc gattacgcca tttgcgccaa
     1141 gtacatcgcc gggactcaag ctaaaccgtt cacccatagc gcgcgcagtg gtcacgcgcg
     1201 tgtggagctg aacgaaatcg acacgttcgc ggccgagccg actgagtccg gattctacgg
     1261 cctgaatcgt ggctcctttg atatcgctta gacagtccgg ttcgagacga atctttgtcg
     1321 agatcacggg cgccacgccc agctgtttca gaatgcgtcc aagattttct tcggatttac
     1381 cgaggccgta ggcataggcg gtatcgaaaa aattgatgcc gcaatcgagc gcatggcgca
     1441 ccgcattgag ttgatcttga tagggcgccc gcaccatcaa gacggcatta ttgccgcacc
     1501 cgaagccgat ctcggacact ttcaacccgg ttttgccaat ggttcgatat tgcatgttta
     1561 ccccgacgag ctctgctgtg agcgtgcgtt caaatgtctg agagttgatt caattccttg
     1621 tgcgacaaat ccccctttca tcccaaagag ggaaagaggg ggattttcgt taaggtcgtc
     1681 tggcgattcg ataactcacc aagattgcgg catcttggct atttagattt tctccaatat
     1741 ctccaacact gcatccgtgt ccaccagatc cgctaagcaa gcgtccggct cccagtaacg
     1801 caattcttcg atcggataac gcccggtgcc aacgagaatg cacttcatgt gattctgccg
     1861 cgcggcttgt aaatcgttcg acgtatcgcc gatgataatg cactgctcgg ccaacaacgc
     1921 gcttcccgtg atatgttcgc atcgcgcttt cgcaatcgcc ggcaaatcag cgcgcacatg
     1981 ggaatcgctc gcgtatgcgc cgcggcaaag atagggcgcc agacctgcgg tttcaagctt
     2041 gatctcggcg gtggcttcga agttgcctgt caccacactc ggacaaatct tttcgttcgc
     2101 cgcaagcgct cgcagcaaag cgtccgtgcc cggcaaggcg cggatcttcg tcgtgccctg
     2161 catcacgtga tggtgatagc gctcgcgcaa attgatcgtg aattgttgcc agatcgtgtc
     2221 ctggaactcg caaacaaagt tcgacttagc aaagatatct tgcacgatca acggatcggt
     2281 gttcccgtcc ggcaccaccg tacgaatgtc gccggtacaa ccgaacgttt ggagaatcgc
     2341 ctcattgaac ggtacatagc cgttttccgt ccttgtgagc gtgccatcga tgtcgaacag
     2401 gatcagccgt tccatgaata tgcggatctt gtatgatcgt ctgctactta gcaagttacg
     2461 tggagactga attgatgatg cagaaaatca ctcacctcgc ttcgaaacat cgaatcgtca
     2521 ttccaagcaa aaacgcagaa tctttccgat ctaaactgcc atgatcttgc accatcgtag
     2581 taattgcgca gcgttgacaa ggcgggtctt tcaagataga agttttccgt aagttttgcg
     2641 cgactggcgt caacgtggaa ttgaccacga gggagcgcaa agcattaaga cggccgaacg
     2701 cctgggtctc ttaataggga ggtccaggct tttttgtgga taatgcggtg tccagttttt
     2761 ttggcgccgt gtttaccatt cctgcccctc ctttaggtct acaagaaggc cttcaaagcg
     2821 tctaggctat tggtcatgat caagcattac ctgctttccc ccggcccgac gcctattccc
     2881 aacgaggttc agttggcaat ggcgcaaacg atgatccatc atcgtacgcc ccaattttat
     2941 caagtgttca atgaaacgcg cgcagggctg aaaaaaatct ttggcaccca aaatgatgtc
     3001 ctcatgctcg cctcctcggg caccggcgcc atggaagctt cagtggcgaa cctgttttcg
     3061 ccgggtgaca aagtcctggt cataaacggc ggaaaattcg gcgagcgctg gctcaatatc
     3121 tcgaacgcct atggtttgaa cccgatcgag ctcaaagtcg agtggggcca ggcggttaaa
     3181 gtcgccgatg tcgagcgcca gttaaaacag cactccgata tccagggcgt catgatccag
     3241 gcgagcgaaa catccactac ggtttttcat cccgttaagg aaatcgccaa acttacggaa
     3301 aacggtccgc tgtttctggt tgatggtgtc accgccgtgg gcgtcgttcc gttgcagatg
     3361 gacgaatggg gtttcgacgt gctggtgacc ggctcgcaga aagcattgat gctgccgcct
     3421 ggtttgggtt tcatcgcgct cagcgagcgc gcctgggaaa agaccaaacg cgccaagtta
     3481 ccgcgctttt attttgacct gaacctggag cgcaagaatc aacagaaagg ttccggcgcc
     3541 ttcaccccgg cagtttctct gatcttcggc gcgcgcgctt ccatgaatct gctcgagcgc
     3601 gaggggttgg caaacgttta tgcgcgccat gctcgtctga gccgcgcgac ccgcgccgcg
     3661 gctacggccc tgggcttgcg actgctggct ccgacggatc ctagtccggc ggcaaccgga
     3721 atctacttgc ccgaaggcat cgacgccgat caggtacttg attacttgcg cgacaaaatg
     3781 aatatcactc tcgccgaagg tcaagatcag ctcaaaggca aagtgatccg cattgcgcac
     3841 gtcggctata tgggatcctt cgatgtcatt accgccattg ccgcgctcga gatggcgctc
     3901 agaaaattcg gtgccgagat accgttcggc cgcggggtgg ccgcggcgga agaagttctg
     3961 atggaggctt tgcaataaaa ttgaggaggg agaatagaga atagaagttg cgcgatcctc
     4021 tatcttcgat cctcgattat cgctcagcat gaagatccta gtcaccgacc ccctcgcccc
     4081 tgaaggtctg gaagttttta aaaacgccga cggcttcgaa gtcgacgtta agatcggtct
     4141 caaacccgac gagctcaaga aaatttgcca tgcctacgac ggttgggtga ttcgcagcgg
     4201 caccaaaatt accgccgatc tgatcgaagc cgctacgaat ctcaaagtca tcggccgcgc
     4261 tggtgtgggc tacgaaaaca tcgacgcgga caccgcgaca aaaaagggca tcgtcgtgat
     4321 gaatacgccg ggcggcaaca atgtgaccac gggcgagcac acgatcgctt tgatgatggc
     4381 gctggcgcgc cacattcccc aggccgtcgc gtctctgaag ggcggcaagt gggatcgcaa
     4441 caagtttgtc ggcgtggaaa tgtgcaacaa gacgatcggc gtcatcggtc tcggcaacgt
     4501 cgggcggatc gtcgccgagc gcgccgccgc actccggatg aaagtcatcg gcttcgatcc
     4561 gttcatcgcc gctgaaaaca tcgctcgcat gggcgtacag ccccgaaccc tcgacgatat
     4621 ttttaccaaa tccgatttca tcacggtcca cgtaccgttg actccggaca cccagggatt
     4681 gatcaataaa gcggctttcg cgaaaatgaa acggggcgtg cgtattatca actgcgcgcg
     4741 cggcggaatt gtcgacgaaa ctgatctcgc cgaagcgatc aaacaaggaa aagtcgccgg
     4801 cgccgcgctc gatgtctacg ttgacgagcc gccgccaccg gatcacccac tactcaagct
     4861 cgatcaggtt atcacgacgc cccatttagg cgcttcgact gatgaggcgc aactgaacgt
     4921 tgcgatcgcc gtcgccgagc agatggttga ttttcttgcc aaaggcgtgg tgcgttatgc
     4981 ggtgaatgtt ccgtcggtca gcccggagct tttacaggta ttgcgtccct atttgactct
     5041 cgccgaaaag ctcggcagtt tgcacgcgca aatggccgga gcgctccccg aagaagtcca
     5101 ggtggaatat cgcggcgatg tcactcaata caatgtcgcg ccgttgacgc ttgccgtctt
     5161 gaaaggtctg ttgtctccag taatggaatt tgccgtgaat tatgtgaatg ccccgattct
     5221 cgctcgagag cgcggcatca agatcatcga gtcaaagggc gaagaggcga ccgatttcac
     5281 cagctcaatc atcgtcagag cgaaaaacgg caaagagagc ttggaagtgg aaggcgccat
     5341 cttcgcggcc aagcatccgc gcatcgtgcg cgtcaatagt ttttatctcg aggcggtgcc
     5401 cgaaggatat attctggttt tgcagaacaa ggatgttcct ggcgtggtcg gcgccgtcgg
     5461 cacgattctg ggcaatcacg gtttgaacat cgccggcatg gagctcggcc gcagtgaaaa
     5521 gggtgggaat gcgatctcgt tcacccatgt cgatgaagcc gtaagcaaaa aag
//
LOCUS       DASULZ010000017         3661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_813332, whole genome shotgun sequence.
ACCESSION   DASULZ010000017 DASULZ010000000
VERSION     DASULZ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813332"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VFY96_00300"
     CDS             complement(<1..929)
                     /locus_tag="VFY96_00300"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX5604920.1"
                     /translation="MLRFKFKLLSNLMIGLLGTVGNGSAYGGFLSVGDHVITLQHADR
                     VRSAIVHVPARATERRDIPVVINFHGGGGHGSNQQEYSLMDRSADRQVFVAVYPNGTG
                     QFSKRLLTWNAGTCCGYAAIHQIDDVGFVRSLTDKIAEVMPIDRRRIYATGLSNGAMM
                     AYRMAAEAGDLIAGIAPIAGGMVLPMIASQRAIPVMHIHSLDDPRALYGGGLGPPFPF
                     TNSRVFHPNIEQMISRWVKHNGCAAEPTVDGQVESKKPRQSARRYVYSNCRKDSEVVL
                     WKLTGAGHVWPGGKQKVLERILGPSTEIIDANQ"
     gene            complement(1006..1368)
                     /locus_tag="VFY96_00305"
     CDS             complement(1006..1368)
                     /locus_tag="VFY96_00305"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HEX5604921.1"
                     /translation="MNSRKAALFTKSPFIASLALGVTLLAGCAGGALTTREKGAGIGA
                     LGGAAMGGIIGSAVHHPAAGAAIGTAFGLGAGALVGDQLQGRDNAAYEQDRAINANQQ
                     ELERQRREIDDLRRRPEY"
     gene            complement(1456..2211)
                     /locus_tag="VFY96_00310"
     CDS             complement(1456..2211)
                     /locus_tag="VFY96_00310"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX5604922.1"
                     /translation="MGLKKPLQRLLSEEALRVVSKLSDEGPQEIFLVLLLAAAMLSSC
                     VVATEQPSRRAPRDDNSSSPASSGARADPRDADRLRRIMLPLLKAMDHPCREDQVRIG
                     IVNQNEINAANAGNCEFYVTMGLLRRANDDQLRGVLAHEIAHEDLGHVAKAQVLGAGL
                     NIGASLLQQLFPAAGVLAPIAGELVARGYGRSEEYAADRHGVDILRRAGYSKETMIDA
                     LTWVRSVAGSGGGGFLSTHPGIDDRITTIQQLR"
     gene            complement(2244..3098)
                     /locus_tag="VFY96_00315"
     CDS             complement(2244..3098)
                     /locus_tag="VFY96_00315"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEX5604923.1"
                     /translation="MRLSKPIVATLVLVVGLYQAGAFGASQTLIRPEAVPVYQPKHFP
                     FEGGEKEVYRASWNGLVSVATAEISTVPTVIDGKKVYQVQVEAKSSKMLDFIWKMRDT
                     ITSTFDAKALLPARFTFSQRENSKVIDTEARLDHTRKRWAVNRQQAGKQPTIYEFDAQ
                     NTLDPITAVYLARSVDFKVGDRLYFKVFGGRYQYLLELFVEKKEPLELASGKIDAYRI
                     IPRIQNLTKKGYAERLNEATIWISADERRLPVRLSSKIAYGSVHLELVQDKRTTESTA
                     VDFNRPAS"
     gene            3288..>3661
                     /locus_tag="VFY96_00320"
     CDS             3288..>3661
                     /locus_tag="VFY96_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5604924.1"
                     /translation="MVKQIRLRLFAVIFSSILFLGFGVLPTSAQDSSPVGARSSALPT
                     LEAAKTCLSNSASAECLDQLFREALKTHSVKEVLQLIEQFEAEDPEFRRDCHPVVHAI
                     GRETFRSKNNLHEAFAACDQTC"
BASE COUNT          807 a         1027 c         1011 g          816 t
ORIGIN      
        1 tcctgattgg cgtcgatgat ttcggtggat ggtccaagga tgcgctccaa cactttttgc
       61 ttgccaccgg gccaaacgtg accggcgccg gtgagcttcc agagcacgac ttccgaatcc
      121 tttcggcagt tggaataaac ataacgcctt gcgctctgcc gcggtttctt gctttcgacc
      181 tgcccgtcaa ccgtcggctc ggctgcacaa ccattgtgct tcacccagcg cgagatcatc
      241 tgctcgatat taggatgaaa cacgcggctg tttgtaaaag gaaatggcgg acccagacct
      301 ccgccgtaaa gggcgcgcgg atcgtcgaga ctgtgaatgt gcattacggg aatggcgcgt
      361 tgagatgcga tcatcggcag aaccatgccc ccggcgatcg gcgcgatccc tgcgatgaga
      421 tcaccggcct cggccgccat gcgatacgcc atcatcgcac cattggaaag tcccgtggca
      481 taaattcgcc gacgatcgat cggcatcacc tcggcaattt tgtctgtgag agaacggacg
      541 aatccaacgt cgtcaatttg atgaatagcg gcatagccgc agcacgttcc cgcattccat
      601 gtcagtaatc gcttgctgaa ctgccccgtg ccattggggt acacggcaac aaacacctgc
      661 cgatcagcac tccgatccat caacgaatac tcctgctggt tggagccgtg gccgccgccg
      721 ccatgaaaat tgataacgac gggaatgtcg cgccgttccg tcgcccgcgc gggaacatga
      781 acaatggcgg agcgtacccg atcggcatgt tgcaatgtga ttacgtgatc gccgacggac
      841 aaaaatccgc catacgctga cccgttgccg actgtgccca gcaagccgat catgagattg
      901 ctgagcagct tgaatttgaa gcgcagcatc ggagaggcta ttttgacttg cgataaaaat
      961 ggggggcact gccccccatt tttatcgcct gatgcaaggc tggaactagt actcgggccg
     1021 gcgtcgcaga tcatcgatct cacggcgctg gcgctcgagt tcctgctggt tcgcgttgat
     1081 cgctcgatcc tgctcataag ctgcgttgtc ccggccttgt aactgatcgc caaccagagc
     1141 tccggcgcca agcccgaagg cggtgccaat cgctgcgccg gcagcaggat gatgcacggc
     1201 cgaaccgata ataccgccca tggcagcgcc gcccagtgcg ccgatgcccg cgcctttttc
     1261 gcgtgttgtc agtgcaccgc ccgcgcatcc ggcgagcaac gtgacgccca gtgccagact
     1321 tgcgatgaaa ggagattttg tgaacaaagc ggcttttcga gaattcatgg tttttgcgcc
     1381 tcctggtgct tagacgtacc cctcccggat ttgtttactg acaggatgaa aagggcgtaa
     1441 accaggtggt agcgattaac ggagctgttg gatggtcgta atacggtcgt caatgcccgg
     1501 gtgcgtggaa agaaatcccc caccgccact gcccgcaacg ctccgaaccc aggtaagcgc
     1561 atcgatcatg gtctctttgg agtagcccgc acgacgcaaa atgtccacgc cgtggcgatc
     1621 ggcggcatat tcttcgcttc gaccatagcc gcgcgccacc aactcgccag caatgggcgc
     1681 cagtacgccg gctgctggaa atagctgttg gagaagggag gcgccgatat ttaaccccgc
     1741 gccaagcact tgcgcttttg ccacatggcc tagatcctcg tgcgcgatct catgtgccag
     1801 tacaccgcgc agttgatcgt cgttggctcg ccgcaataaa cccatggtga cgtagaattc
     1861 acaattcccc gcattcgctg cattaatttc gttctggttt acaatcccga tacggacctg
     1921 atcctcgcga cagggatgat ccatcgcctt gaggagcggc aacatgattc gacgcaatcg
     1981 gtcggcatcc cgcggatcgg ctcgggctcc ggaagaagcc ggggaggaag aattgtcgtc
     2041 cctgggagcg cgccggctgg gttgctccgt ggcgactaca caggaggaca acatggcagc
     2101 agcaagcagc aaaaccagga agatttcttg cggtccctcg tcgctgagtt tcgacacgac
     2161 ccggagcgct tcttcggaca aaagacgttg cagcggtttt tttagtccca tcacatagat
     2221 ggaacaacgt ggcggcggtc gggtcaagag gctgggcggt taaagtcgac tgcggtcgat
     2281 tcggttgtcc gcttgtcttg caccagttcc aaatggacac tgccataagc gattttgctg
     2341 ctgagcctaa ccggcaaccg gcgctcgtcc gcagaaatcc aaatcgtcgc ctcgttcaag
     2401 cgctcggcat atcccttctt cgtgaggttc tgaatccgcg ggatgattcg gtatgcgtcg
     2461 attttccccg aagcaagctc taggggttct ttcttctcca cgaacaactc cagcaaatac
     2521 tggtaacgcc cgccgaaaac cttgaaataa agccggtccc cgactttgaa atcgacgctt
     2581 cgcgcgagat agacggcggt aatgggatcg agggtatttt gcgcgtcgaa ctcgtagatc
     2641 gtcggttgtt ttccggcctg ctgccggttt actgcccatc gcttccgcgt gtgatcgaga
     2701 cgcgcttcgg tatcgatgac cttcgaattc tcccgttgac tgaacgtgaa gcgagccggc
     2761 aacaacgcct tggcatcgaa agtcgaggtg atggtatcgc gcattttcca gataaaatcg
     2821 agcatcttcg agctcttggc ctcgacttga acctggtaaa cttttttgcc atcgataacg
     2881 gtcggaaccg tcgaaatttc agcagtggcc accgagacta ggccgttcca ggaagctcga
     2941 taaacttctt tttcgccgcc ttcgaacgga aaatgcttgg gctggtaaac cggcactgcc
     3001 tcgggcctga tcagtgtttg actcgcgcca aaagcgccgg cttggtagag accaaccaca
     3061 agaacaagcg tcgcaacgat cggcttggat aggcgcatta caggtcttag acgaacggac
     3121 ggtcggtgag ttatcacaaa gtaacacaca aaatgatgct aagtaaaatt ctaaccgcat
     3181 ttggttgcgt atattcgatt gaacgattga gaatacgatt tgttttgggc gaaaaaaatt
     3241 gcagagagtg atcgatttag gctagtatcg gccaacgggg agtattcatg gtgaaacaaa
     3301 ttcgcctgcg gctattcgca gttatttttt ccagtatcct ttttcttggt tttggggtgc
     3361 tgcccacttc cgcgcaagat tccagcccgg tgggggctcg atcctcagca cttccgacct
     3421 tagaggcagc aaagacctgc ctgagcaaca gtgcgtctgc cgagtgtctc gatcaacttt
     3481 ttcgcgaagc gctgaagacc cactcggtca aggaagtttt gcagctgatt gaacaattcg
     3541 aggcggaaga tccggaattc cggcgtgact gtcacccggt ggtgcatgct attgggcgag
     3601 aaacgtttcg ctcaaaaaat aaccttcacg aggcgttcgc ggcttgcgat cagacctgcc
     3661 a
//
LOCUS       DASULZ010000018         5474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_915022, whole genome shotgun sequence.
ACCESSION   DASULZ010000018 DASULZ010000000
VERSION     DASULZ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915022"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1231
                     /locus_tag="VFY96_00325"
     CDS             <1..1231
                     /locus_tag="VFY96_00325"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEX5604925.1"
                     /translation="APEHKLRIVSILKQMGEIVAVTGDGVNDAPALKKADIGIAMGLR
                     GNDVAKEASRMILTDDNFSSIVAAIEEGRAIFDNIKRFAAYVLNSNPQEMYPYLFWVL
                     FPGMPLAMTVMGVLAVDVGTDLIPAMGLGIEPPEEGIMERSPRRRDERILSLAFILRS
                     YFVQGTLLAASCYATYYYMGWVLGAWRPGESLTNMPASPGGLKFETASYAYLQTLTAY
                     FFPTVTTQIANVLCKRSWRSSLFSINFLNPARRQEALQAILTWRPPHYTARVRIDYHV
                     KGVTEFDIVRAFFALAAGLIALPFRFVSMLFVQSLARIERPIIVPFTAWLAGFLKRHY
                     IIFNFISNPLIDLGILFELVLCYFFFYTPLAEIYYFAPVPWHVYLFAFHGTIVLIAFE
                     EAKKYYRRKGCPLEFLG"
     gene            1344..2219
                     /locus_tag="VFY96_00330"
     CDS             1344..2219
                     /locus_tag="VFY96_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX5604926.1"
                     /translation="MSLLFAFSLVLHTDAASQDHAPIADQLIGGELSYIVRRGDSLTG
                     IGARFGVAVGVLAADNDVSPSALLKTGQQLRVDNHHIVPNIVSDGIVINIPQRMLFYF
                     KERRLIRAFPVGLGRYDWPTPTGAFQIVAKKENPIWSVPISIQKEMQREGKSVQTCVP
                     PGPDNPLGKHWLGLSIAGYGIHGTIAPASIYHFQTHGCIRAHPDDIAALFDDLSPGTH
                     GLLVYQRLLIANVGGKVFLEVHPDPYKKDPDVRGQFEKLLKNLSGFSFDRQLAARIID
                     SQDGIARQIGVNSLR"
     gene            2249..2800
                     /locus_tag="VFY96_00335"
     CDS             2249..2800
                     /locus_tag="VFY96_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HEX5604927.1"
                     /translation="MKREPQLNRRYFLRLSAIAATAALFPRTAFSIDARIPEKSLAFY
                     NTHTGETLKSVYWADGRYLGDSLRAINHILRDPRNNEIHDIDTRLLDLLFSLRREVDT
                     SQPFHVISGYRSAETNTYLRTHTTGVTENSLHLVGQAIDIRSPGRELRSLQKAAIQLR
                     GGGVGYYPKSDFVHIDIGRVRYW"
     gene            complement(2893..5439)
                     /gene="glgP"
                     /locus_tag="VFY96_00340"
     CDS             complement(2893..5439)
                     /gene="glgP"
                     /locus_tag="VFY96_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020161015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX5604928.1"
                     /translation="MSKQTRDLSPLYSFLPAEIEGFDELAELALDLRWSWNHYADELW
                     RQFDAKLWGITQNPWVILQTVSRDQIQRMLADPTFRKKVDALVEARREAAEAPAWFQQ
                     KYQRTSLSCVAYFSMEFMLSEALPIYSGGLGNVAGDQLKAASDLGVPVIGVGLLYQQG
                     YFRQVIDKDGAQQALFPYNDPGQLPIIPLRQANGEWLRVEIALPGYSVWLRAWEVRVG
                     RAKLYLLDSNDAANFPAHRGITSELYGGGPELRLKQELTLGIGGWRLLEALGIQPEVC
                     HLNEGHAAFAVLERARSFIKKTGQSFEVALAVTRAGNLFTSHTAVAAGFDRFAPDLIE
                     QYLSEYAQKELGLTVHELLALGRQNPNDSEEHFNMAYLAIRGSGAVNGVSRLHGKVSR
                     RLFERLFPRWPEDEIPVGYVTNGVHMPSWDSAAADELWTTACGKDRWLGTTETLERDI
                     RRVSDGELWEMRTHASRSLVVYARERLSQLLAASGSSDEAVETAKHLFDPNVLTLGFA
                     RRFATYKRPNLLLYDPERLTRLLTDAHRPVQLIIAGKAHPDDQAGQAMIQEWTRFIRR
                     PETRRHAIFLSDYDMLLTEHLVQGVDLWINTPRRPWEACGTSGMKVLVNGGINLSELD
                     GWWAEAYTPEVGWALGDGQEHDADPSWDAAEAQALYELLEREVIPEFYTRDENGIPRA
                     WVARMRESMARLTPRFSANRTVREYTEQHYLPAATGYRTRAADKSAVGREVVDWQHSL
                     NQKWAMLHFGEARVETVGDLHVFDVQLYLGDVDPDRVRVELYADGVNDSGPVRQEMKR
                     VRQLVGAAGGYAYSARVDATRPGTDYTARVIPYCAGVAIPLETDRILWQR"
BASE COUNT         1181 a         1635 c         1407 g         1251 t
ORIGIN      
        1 cgcaccggag cataagctcc gcatcgtctc catcctcaaa caaatgggcg agatcgtcgc
       61 cgtcaccggc gacggcgtca acgatgctcc agcgctaaaa aaagcagaca tcggcattgc
      121 catgggactg cgcggcaacg atgtcgctaa agaagcctcg cgcatgatct tgaccgacga
      181 taactttagt tccatcgtcg cggccatcga agagggccgc gcaattttcg acaacatcaa
      241 acgctttgcc gcctacgtgc tcaatagcaa tccgcaggaa atgtatccgt accttttttg
      301 ggtcttattc cccggcatgc cgctggcaat gacggtcatg ggcgtgttgg cggtcgatgt
      361 gggaaccgat ttgataccgg cgatgggcct gggcatcgag ccgccggaag aaggcatcat
      421 ggagcggtcg ccacggcgcc gcgacgaaag gatcttatcg ctcgctttca tcctgcgctc
      481 ttattttgtc caaggcactc tgctcgccgc cagttgctac gctacttact actatatggg
      541 ctgggtcctg ggcgcgtggc gacccggcga gagtcttaca aatatgccag cgtcgcccgg
      601 cggcctcaag tttgagaccg cgtcctacgc ttacttacag actttgacgg cttacttttt
      661 cccaacggta acgacgcaaa tcgccaatgt gctctgcaag cgctcgtggc gcagttcgct
      721 cttttcaatt aacttcctca accctgcgcg ccgccaagaa gcgctgcaag caattttgac
      781 ttggcgtccg ccgcactata cggcgcgggt gcgcatcgat taccacgtca aaggcgtcac
      841 agaatttgac attgtgcgcg ccttcttcgc gctcgcagct ggtttgatag ccttgccatt
      901 ccgtttcgtt tcgatgctct tcgttcagtc actggcgcgc atcgagcgcc cgatcatcgt
      961 tccgtttacc gcctggctcg cgggttttct caagcggcac tacattatct ttaatttcat
     1021 ctccaatccg ctcatcgacc tcggtattct gttcgaactc gtgctctgct atttcttctt
     1081 ctacacacct ctcgccgaga tctattactt cgccccggtg ccgtggcacg tctacctctt
     1141 tgcctttcat ggcacgattg tgctgatcgc tttcgaagaa gcaaaaaaat actaccggcg
     1201 gaaaggctgc ccgctggaat ttctcggttg aattcacggg gaaaactatg gctgagcatg
     1261 tctggcttcg tgggagacct tcttcacgca agaggatagc ttgcgacaag aaatttgggc
     1321 ttattagtct cattttcccg atcgtgtctc tcctgtttgc tttctcgctc gttctgcata
     1381 ctgatgcggc gagtcaagac catgcgccaa ttgcggatca actcatcggc ggcgagcttt
     1441 cttacatcgt tcggagagga gattcactca caggcatcgg cgcaaggttc ggcgtagccg
     1501 tcggcgttct cgccgcagac aatgacgttt ccccaagcgc gcttttgaaa acaggacaac
     1561 agctgcgtgt cgacaaccat cacatcgtac ccaatatcgt cagcgacggg atcgtgatca
     1621 acattccgca gcgtatgctc ttttatttca aggagcgccg tttgattcgt gcgttccccg
     1681 tgggactcgg gcggtatgat tggcccactc ccacgggcgc atttcaaatc gtcgcaaaaa
     1741 aagaaaatcc gatctggagc gttccaatat caatccagaa ggaaatgcag agagaaggca
     1801 aatctgttca gacttgcgtt ccaccaggac cagacaatcc tctgggcaag cattggctcg
     1861 gacttagcat cgctgggtat ggcattcatg gtacgatcgc gccggcaagc atctatcact
     1921 tccagaccca tggctgcatc cgcgcgcacc cggacgatat cgccgcactt tttgatgacc
     1981 tgtcgcccgg tacgcacggc ttgctggttt atcagcgact tctcatcgcg aatgtcggcg
     2041 ggaaagtttt tttggaggtc catcccgatc cgtataaaaa agatcccgat gtccgcgggc
     2101 agtttgaaaa gttactcaaa aatctatctg gctttagctt tgatcgtcag ctggcagcgc
     2161 gtataatcga ctcgcaggac ggtatagccc gacagattgg agttaacagt ttgcgatgaa
     2221 ttctttaggt cccgatcacg aatccgtgat gaaacgtgaa ccgcagctaa atcgacgata
     2281 ctttttaaga ctcagtgcaa ttgcggcaac agcggcactt ttcccccgca ccgccttcag
     2341 cattgacgcg agaattcccg aaaaatcttt agcattctac aatacccata ccggcgaaac
     2401 cctgaaatca gtttattggg ccgatggccg atatctggga gattcgctcc gtgcgatcaa
     2461 tcacattttg cgcgatccac gcaacaacga gattcatgac atcgatactc gactcttgga
     2521 tctccttttt tcgcttcgcc gcgaagtcga tacaagccaa ccctttcatg tcatttctgg
     2581 ctaccgctcc gcagaaacca acacatatct gcgcacgcac accaccggag tcaccgaaaa
     2641 cagcctacac ctcgtcggac aggctatcga catcaggtca ccggggcgag agctgcgcag
     2701 cttacaaaaa gctgccatcc agttgagagg cggcggagtt ggttattacc caaagtcgga
     2761 tttcgtgcac atcgatatcg gacgcgtgcg ctattggtga actgtttcta cttactagcg
     2821 caataacggc gtctctattt cggggcagcg aggacacgtg catcgtccgg cgcgatcggc
     2881 ctcttcctaa attcatcgct gccataggat acgatcagtc tccaggggaa tcgcaacgcc
     2941 agcgcaatac ggtatgacgc gcgctgtata gtccgttccc ggccgcgttg catccacccg
     3001 cgcgctataa gcgtagccgc cggccgcgcc caccagttgg cggactcgct tcatctcctg
     3061 ccggacgggt cccgagtcgt tgactccatc ggcataaagc tcaacccgca ctcgatccgg
     3121 atcaacatca ccgagataaa gttgaacgtc gaacacgtgc aggtccccga ccgtctcaac
     3181 cctcgcttcg ccgaaatgca gcatcgccca tttctggttt agagagtgct gccaatcgac
     3241 cacttccctg ccgactgcac ttttgtctgc agcgcgtgtt cgatagccgg tggcagccgg
     3301 gaggtagtgt tgctcagtgt attcgcgcac cgtccggttg gcagaaaagc gcggtgttaa
     3361 cctcgccata ctttcacgca tgcgcgccac ccaggcacga ggaataccgt tctcgtcgcg
     3421 ggtataaaac tcggggatca cctcccgttc cagcagttcg taaagcgcct gcgcttcagc
     3481 cgcgtcccag gatggatcgg cgtcatgttc ctggccatcg cccaacgccc accctacctc
     3541 cggtgtgtac gcttcggccc accagccgtc cagttctgaa agattgatgc cgccattgac
     3601 gagcaccttc atcccgcttg tcccacacgc ctcccaaggc cgccgtggcg tattgatcca
     3661 gaggtccacg ccctgcacta gatgctcagt caaaagcatg tcgtagtcac tcaaaaagat
     3721 cgcatggcgt cgcgtttcgg gccgccgtat gaagcgcgtc cattcctgaa tcatggcctg
     3781 ccccgcctgg tcatcaggat gggctttccc ggcaatgatg agttgtacag gacgatgagc
     3841 gtccgtcagt aaacgggtca ggcgttctgg gtcgtacagc aacaaattgg ggcgcttata
     3901 ggttgcgaag cgacgcgcaa atccgagcgt caatacgttg ggatcaaaca gatgctttgc
     3961 agtttcgacg gcctcgtcag acgaacccga tgcagccagt agctgggaca atcgttcgcg
     4021 agcgtatacg acaagagacc ggctagcatg ggtgcgcatt tcccagagct cgccgtcgga
     4081 aacacgtcga atgtcgcgct ccagtgtttc tgtcgtgccg agccagcgat cctttccaca
     4141 ggcggttgtc caaagctcat cggccgccgc cgaatcccaa cttggcatat ggacgccgtt
     4201 ggtcacgtat ccgaccggga tttcatcttc cggccagcgc ggaaacaggc gctcaaacag
     4261 gcgccggctg acctttccgt gcaaacggct cacaccgttg accgccccgc ttccacggat
     4321 tgcgagatac gccatgttga aatgctcttc cgagtcgttt gggttttggc gtcccaaggc
     4381 cagcaactca tgaaccgtca ggccgagttc cttctgggca tactcactaa ggtattgctc
     4441 gatcaagtcg ggagcgaacc ggtcaaaacc ggcggccacc gcagtgtgac tggtgaagag
     4501 gttacccgct cgcgtgacgg ccaatgcaac ttcgaaggac tgtcctgtct ttttgatgaa
     4561 gctccgcgcg cgttctaaca cggcaaaagc tgcgtgtcct tcatttaggt gacagacttc
     4621 cggctggatg ccgagcgctt cgagcagccg ccacccaccg atgccaagag tcagctcttg
     4681 cttgaggcgc agctcgggtc ctccaccata cagttcgctg gtaatccctc gatgagcagg
     4741 aaagttcgcc gcgtcattgc tatccaatag ataaagcttc gctctgccaa cccgtacctc
     4801 ccaggcgcga agccagaccg agtaaccggg caacgcgatc tccacccgca accactcacc
     4861 attcgcctga cgcaaaggta tgattggcag ttgccccgga tcgttgtaag ggaagagggc
     4921 ctgttgcgcc ccgtccttgt caatcacctg gcgaaaatag ccttgttggt agagcagtcc
     4981 cacaccgatg accggcacac ccaaatcgct ggcagctttg agctgatcgc cagcgacatt
     5041 gccaagcccg cccgagtaaa tcggcagcgc ttcgctcaac atgaattcca tactgaaata
     5101 ggcgacacag ctcagagaag tccgctgata tttttgttga aaccacgcgg gcgcctccgc
     5161 cgcttcacgt ctagcttcca caagagcatc gactttcttg cggaacgtag ggtcagccaa
     5221 catgcgttgt atttggtccc gcgagacagt ctgcaggatg acccatgggt tttgggtgat
     5281 cccccatagc ttggcatcaa actggcgcca cagttcgtcg gcgtaatgat tccacgacca
     5341 acgcagatcc agggcgagct ctgccagctc atcgaaccct tcaatttccg ccggtaggaa
     5401 actgtagagt ggacttaaat cgcgtgtttg tttgctcatt ggctttactc agacaaagca
     5461 ctggacgagc tgtc
//
LOCUS       DASULZ010000019         2844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1786, whole genome shotgun sequence.
ACCESSION   DASULZ010000019 DASULZ010000000
VERSION     DASULZ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1786"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VFY96_00345"
     CDS             <1..406
                     /locus_tag="VFY96_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5604929.1"
                     /translation="IEAQIADLRTHSQTEQMMIVIDDCQRLKGVDHAWDTRLQIIAEE
                     LQQTARKFNLPVLAVWPDLASGSGALAQSWSEKAASPDVVLVMDRDATRTNAMNELRQ
                     AIILHLVKNRGGEKGRLAYDFATAFAAFTETE"
     gene            complement(390..1115)
                     /locus_tag="VFY96_00350"
     CDS             complement(390..1115)
                     /locus_tag="VFY96_00350"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEX5604930.1"
                     /translation="MIWTSAAADLRLGADEIHVWRVDAFNNDADYAYCAALLSKDEQA
                     RGEQFKFDRDRRLFTVAHAALRTICATYLRTTPRSIEFANGPYGKPKLASDSRSGLRF
                     NLSHSGELALIAAALQREIGVDIEAVRDFDFDQVAERFFTAREVAALRSLPKPLQRSA
                     FYKCWTSKEAFLKARGTGLSAKLDEVQIICDGDDIRISAFVPGWSLAALSAGEGYIAA
                     LAGQGAPVRVQLCQWSSTSTLSQ"
     gene            complement(1209..1511)
                     /locus_tag="VFY96_00355"
     CDS             complement(1209..1511)
                     /locus_tag="VFY96_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5604931.1"
                     /translation="MATQDEEAVAIENAAGLIIAVTEQRNWIEVFFEGDLMHTRTVNF
                     PSGRVFDIYVEDIPHKTTVYEHPRTMIFFNGPCHLQITRDGDRVIVTGSTSRQNLA"
     gene            complement(1524..>2844)
                     /locus_tag="VFY96_00360"
     CDS             complement(1524..>2844)
                     /locus_tag="VFY96_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5604932.1"
                     /translation="TAIPLSYFATQKIGAVTVILDARLKGKELQAVLKDADLKLLIVH
                     AQLLGEIEEAFKGMTTIPLWVVGGEGERSFEKRLCAPGGAIESPRVNSGDDALILYTS
                     GTTGEPKGVVLSYQNLAQYPQVMRKMGITDGSTIRGCILPMSHIVGPVVCNELAERGY
                     TLVIFDQINPVTLLEGIQRYRVTVFESVPIVFQLLLGVKNLASYDMSSVKVAAMMGTS
                     IPMPLLNTFQTAQSHIKVIQGYGLTETSPMITLVEPEMAKEKIGSIGQAVPGVEVKIV
                     DENGQELGNEEPGEIITRGPHVMKGYFRRPDATAARIREGWLYTGDVGKRDRDGYFYH
                     LGRRDDMIITGGLNVYPAEVENLIYTYPGVQEAIVFAIPDIKRGHVIGAAVVPHGGAN
                     LVDKELLAFLRANLANFKVPDKIVVREWLPRTSSGKTVRDAAALLSH"
BASE COUNT          632 a          818 c          763 g          631 t
ORIGIN      
        1 aattgaagcg caaatcgccg acctgcgtac tcacagccaa acagagcaaa tgatgattgt
       61 cattgacgat tgtcagcgtc tcaagggcgt ggatcacgca tgggatacgc gtttgcagat
      121 catcgccgag gagctgcaac aaaccgcccg gaagttcaac ctgccggtgc tcgcagtttg
      181 gcccgatctc gcgagcggca gcggtgcact tgcgcaaagc tggagtgaaa aagccgcctc
      241 tccggacgta gttctggtta tggatagaga tgcaactcga accaacgcca tgaacgagct
      301 acgtcaagcg attattttgc acctggttaa aaaccgcggc ggtgagaaag gccggctggc
      361 ttacgacttc gcgaccgcat tcgccgcatt cactgagaca gagtagaagt cgaactccat
      421 tgacagagct gtactctgac gggcgctccc tgaccggcga gtgccgcgat atagccttct
      481 ccggcagaca atgccgccaa tgaccagccc ggtacaaacg cgctgatccg aatatcgtca
      541 ccgtcgcaga taatctgaac ctcgtccagt ttcgcggata agccggtgcc tctcgccttt
      601 aaaaaagcct ccttgctcgt ccagcatttg taaaacgctg aacgctgtag aggctttgga
      661 agagagcgca aagcggccac ttcccgggcg gtaaaaaaac gctcggcaac ttgatcgaaa
      721 tcaaaatctc tgacggcctc gatatccact ccgatctcgc gttgcaaagc ggcggcgatc
      781 agcgccaatt ctcctgaatg ggagagattg aaccgcaggc cactgcgcga gtccgatgcc
      841 agtttgggct tgccgtacgg accgttggca aactcgatgc tgcgcggggt tgttcgcaag
      901 taggtcgcac agatcgtgcg cagcgcggcg tgggcgacgg tgaaaaggcg ccgatctctg
      961 tcgaatttga attgctcccc gcgagcttgt tcgtcctttg acaagagagc cgcgcagtaa
     1021 gcgtaatcgg cgtcattgtt aaaagcatca acccgccaaa cgtggatttc gtctgcgccg
     1081 agtctaagat cagcggcggc gctggtccaa atcattgctg gcgatatatt cgtatttttt
     1141 cctcagccag ggctcaacca accgcacctg acgcggtcgt caagtccggc cgatggctgg
     1201 tttctggatc acgccaggtt ttggcgtgac gtgctaccag taacgatgac tcggtcgccg
     1261 tcgcgcgtaa tctgcagatg gcacggtcca ttgaaaaaaa tcatcgtccg tgggtgttca
     1321 tagacggtcg tcttgtgcgg aatatcttcg acatagatgt cgaacacgcg gcccgacgga
     1381 aaattaaccg tccgggtgtg catcagatcg ccttcgaaaa acacttcgat ccagtttcgc
     1441 tgttcggtga ctgcaatgat caacccggcg gcattttcga tcgccaccgc ttcttcatct
     1501 tgagtcgcca cgaacggagc ccttcaatgg ctgagtaatg ccgcggcatc tcgcaccgtc
     1561 ttgcctgagg aggtgcgcgg tagccattcc cgcaccacga ttttgtccgg gactttgaag
     1621 ttcgccagat tggcgcgcaa aaaagcgagg agttccttgt caaccagatt ggcccctcca
     1681 tggggcacga cagcggcgcc gatcacgtgg ccgcgcttga tatctggaat cgcaaaaacg
     1741 atcgcttcct gtacacccgg ataggtgtag atcaaattct ccacctctgc cgggtaaaca
     1801 ttcagcccgc cggtaatgat catatcgtcg cgccgaccga gatggtaaaa atagccgtct
     1861 cggtcgcgct tgccgacatc gccggtgtag agccaaccct cgcgaatacg cgccgcagtc
     1921 gcgtccggcc ggcgaaagta gcccttcatg acatgcgggc cgcgtgtaat gatttcaccc
     1981 ggctcctcat ttcccagctc ttgcccattt tcatcgacaa ttttgacttc gacgccgggc
     2041 acggcttggc cgatgctgcc gattttttcc ttggccatct cgggctccac gagagtgatc
     2101 atcggcgagg tttccgtcaa tccatagccc tgaatgacct tgatatgaga ttgtgcggtt
     2161 tgaaacgtgt tcaacagcgg catcggaatg ctcgtgccca tcatggcggc tactttgacg
     2221 ctgctcatgt cgtagctcgc taggtttttt actcccaata acaattggaa aacgatcggc
     2281 acgctctcga agactgtcac gcgatatctt tgaatcccct cgagcaacgt aaccggattg
     2341 atctgatcaa aaatcaccag ggtatagcct cgttcggcga gctcattgca aaccactggc
     2401 ccgacgatgt gcgacatcgg cagaatgcag ccgcgaatgg tcgatccgtc cgtgatgccc
     2461 atcttgcgca tcacctgagg atactgagcg aggttttgat aacttaaaac cacacccttg
     2521 ggttctccgg tggtgccgga agtatagaga atcagcgcgt catcaccgga atttacacgg
     2581 ggagactcaa ttgcaccgcc gggcgcgcag agtcgctttt cgaagctccg ttcgccttcg
     2641 ccaccgacga cccagagcgg tatcgtcgtc atccctttga aggcttcttc gatctcgccc
     2701 aacaactgcg cgtgcacaat caacagtttc aggtcggcat cctttagaac cgcttggagc
     2761 tccttgcctt tgagccgggc atcgagaatt accgtcacgg cgccgatctt ctgagtggcg
     2821 aaatagctca acggaattgc cgtc
//
LOCUS       DASULZ010000020         4242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_712346, whole genome shotgun sequence.
ACCESSION   DASULZ010000020 DASULZ010000000
VERSION     DASULZ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712346"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1337
                     /locus_tag="VFY96_00365"
     CDS             117..1337
                     /locus_tag="VFY96_00365"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020471643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEX5604933.1"
                     /translation="MSWITMIKTPIYLDNHATTPVDPRVLTAMLPFFTKKFGNASSKN
                     HPFGWEAEAAVDSAREQVARLIGASSPREIVFTSGATESDNLAIKGVAQAYRERGNHI
                     VTCTTEHKAVLDSCKALEKQGFKVTYLRVQTNGLVNLQRLEDSLTDKTILVSIMTANN
                     EIGTIQPVEEIGRLTKKKGILFHTDATQAVGKVPVDVNKMAIDLLSLTAHKIYGPKGV
                     GALYVRGTNPRVKLMPMLDGGGHERGMRSGTLNVPGIVGLGIACELGQKEMGGEAERL
                     IGLREHLRTGLFAELEDVYINGDPVKRLPGNLNMSFGYIDGESLMMGLKEIAVSTGSA
                     CTSASLEPSHVLKALGLEDSLAHASIRFGVGRFNTAEEIEYTIGRVVEEVRRLRKISP
                     LYKARAEKRKVGVF"
     gene            1352..1681
                     /locus_tag="VFY96_00370"
     CDS             1352..1681
                     /locus_tag="VFY96_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX5604934.1"
                     /translation="MAMGVSLTERAVARIKELIAAEQRDGQGLRVKVVGGGCSGLQYK
                     VDFDAPKPTDKVFERDGAKVLVDMKSLLYLTGTELDYKDELMQSGFVFQNPNVKQSCG
                     CGASFTV"
     gene            1696..3156
                     /gene="sufB"
                     /locus_tag="VFY96_00375"
     CDS             1696..3156
                     /gene="sufB"
                     /locus_tag="VFY96_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX5604935.1"
                     /translation="MKTETETVQDLTRREYKYGFITDIEADTVSPGLNEDIVRLISAK
                     KEEPEFMLQWRLKAYRHWAKLEKAEAEPKWAMVHYPPIDYQSIRYYSAPKLKGDRPKN
                     LEEVDPELIKTYEKLGIPLREQELLAGVAVDAVFDSVSVATTFKSKLADLGIIFCAFS
                     EAVQNHPELVEKYLGSVVPYTDNFFAALNSAVFSDGSFCYIPKGVRCPMELSTYFRIN
                     AAETGQFERTLIIADEGATVSYLEGCTAPMRDKNQLHAAVVELIAHRDAQIKYSTVQN
                     WYPGDAEGKGGIYNFVTKRGKCLGERSKISWTQVETGSAITWKYPSCILQGDDSVGEF
                     YSVALTNNYQQADTGTKMIHIGKNTKSTIVSKGISAGHGQNTYRGLVKIMKPAKGARN
                     YSQCDSLLLGDQCGAHTFPYLEVFNNSSQLEHEASTSKIGEDQLFYCRQRGISPEDAV
                     NMIVNGFCKQVFKELPMEFAVEAQKLLGVSLEGSVG"
     gene            3182..>4242
                     /gene="nifS"
                     /locus_tag="VFY96_00380"
     CDS             3182..>4242
                     /gene="nifS"
                     /locus_tag="VFY96_00380"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009784202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HEX5604936.1"
                     /translation="MAHQVYLDNNATTRVLPEVAEAMLPFLTEHYGNPSSIHRFGSGV
                     GERIAVAREQVANLIGAADPVEIVFTSCGTEGDNAAIRGMLEARPNKRHIVTTQVEHP
                     AVLSLCQHLEKRGYRVTWLSVDENGGLDLEELRSALTDDTALVSVMWANNETGVIFPV
                     EQIGAIVKGKGVPFHVDAVQAAGKIHLRVQDLPVDLMTISGHKFYAPKGVGALYVKRG
                     LTFPPFIIGGHQERNRRGGTENVASIIGMGKAAEVASSRIDSDAARITNLRDQLEALL
                     LRSCPGSRINGRAQKRLPNTCNISFRYLEGESILVLLDQQGICASTGSACTAGSSEPS
                     HVLRAMKVPPDWLQGAVRF"
BASE COUNT         1029 a         1039 c         1253 g          921 t
ORIGIN      
        1 tcgcgtcccg ggcatctctc gataattaag caaaaacaac agcttagttt cgagtaggcg
       61 gcgttgactc gcgagcatat tttggttacc ttgatatcag accaataaag tctgtaatgt
      121 cctggatcac aatgatcaag acgccgatct acttggataa tcatgccaca acccccgtgg
      181 acccgagggt attaaccgcg atgctgcctt tttttactaa aaagttcggc aacgcttcga
      241 gcaagaatca cccgttcgga tgggaagccg aggcagccgt agatagcgcc cgggagcagg
      301 tggcgaggct aattggcgcg tcttcgccgc gggagattgt ctttaccagc ggtgccactg
      361 agtcggataa tttagcgatc aaaggggtgg cgcaggctta tcgggagcgc ggaaatcata
      421 tcgtcacctg tacgacggaa cacaaagcgg tcttggacag ttgcaaggct ttggagaagc
      481 agggattcaa ggttacttac ctgcgggtgc aaaccaacgg cttggtcaat cttcaaaggc
      541 tcgaggattc gcttactgac aagacgattc tcgtttcgat tatgacggca aacaacgaga
      601 tcggcacgat ccagcccgtg gaggaaatcg gtcgactaac caaaaaaaag ggcattctgt
      661 ttcataccga tgcgactcag gcggtgggaa aagttcccgt cgacgtaaat aaaatggcca
      721 tcgatctgct gtcgctcacc gcgcacaaga tctatggacc gaaaggtgtc ggcgcgcttt
      781 acgtccgagg tacaaacccg cgggtgaaac taatgccgat gctcgatggc gggggccacg
      841 agcgcggcat gcgatcggga acgttgaatg tgcccggcat tgtcggcttg gggatcgctt
      901 gcgaactggg acaaaaggag atgggtggtg aggccgaacg cctgataggg ttgcgagaac
      961 acttgcggac gggtctattt gcggaactcg aagacgttta tatcaatggc gatccggtga
     1021 agcgcttgcc gggaaattta aatatgagtt tcggttatat cgacggagag tcgctgatga
     1081 tgggcctcaa ggaaattgcc gtgtcgacgg gttcggcatg cacgtcggca agcctcgaac
     1141 catcacacgt cctcaaggcg ttaggtttgg aggactcttt ggcgcatgct tcgatccgtt
     1201 ttggcgttgg ccgattcaac accgctgagg agatcgaata cacgatcggg cgtgtcgttg
     1261 aagaagttcg tcgcctcagg aaaatttctc ccttgtacaa ggcaagggcg gaaaaacgca
     1321 aggttggagt tttctaggag gaaaatgcgc gatggcgatg ggagtgagtc ttacggagcg
     1381 tgcggtggcg cgaattaaag aactgattgc agctgagcag cgggacggtc aaggcttgag
     1441 agtgaaagtc gttggcggcg gctgctcagg attgcaatac aaagtcgatt tcgatgcacc
     1501 taagcccacg gataaagttt ttgaacggga tggagccaag gtgctggtgg acatgaaaag
     1561 cttgctttat ctgaccggca cggagctcga ttacaaagac gagctgatgc aatccggctt
     1621 cgtttttcaa aacccgaacg tgaagcaatc ctgcggctgc ggggcatcat tcacggtttg
     1681 attaaggatt tggcgatgaa aactgagacc gaaactgttc aagacctgac tcgacgcgag
     1741 tacaagtacg ggtttatcac cgatatcgag gcagataccg tatcgccggg cctgaacgaa
     1801 gacatcgtgc ggcttatttc cgccaagaaa gaagaacctg agttcatgct tcaatggcgc
     1861 ctcaaggcct accggcattg ggcgaagctg gaaaaagccg aggccgagcc aaagtgggcg
     1921 atggtgcatt atccgccgat cgattaccag tcgattcgct actactcggc gccaaagtta
     1981 aaaggcgata gaccgaagaa tctcgaggag gtcgaccctg agctcatcaa aacctacgag
     2041 aagcttggga ttccgctaag agagcaagaa ctgctcgcgg gcgtggcggt ggatgcggtc
     2101 ttcgatagtg tttcagtggc aaccaccttc aaaagtaagc tcgcggacct ggggataatc
     2161 ttctgcgctt tttccgaagc cgtacaaaat catccggagc tagtggagaa gtatttgggt
     2221 tcggtggtgc cgtacacgga caattttttt gcagcgctga attcggcggt ctttagcgac
     2281 ggctcttttt gctacattcc caagggcgtg cgctgtccca tggagctgtc gacctatttc
     2341 cgcatcaacg cggcggaaac gggccagttc gagcgcacac tgatcattgc cgatgagggt
     2401 gcgaccgtca gctaccttga aggttgcacc gcgccgatgc gtgataagaa ccagttgcac
     2461 gctgcagtgg tggagctcat cgcgcaccgg gatgcgcaaa tcaagtattc aaccgtgcaa
     2521 aattggtatc cgggagacgc cgaaggcaaa ggcggcatct ataattttgt caccaagcgc
     2581 ggcaaatgcc tgggcgagcg ttcgaagatc tcatggacgc aggtggaaac cggttcggcg
     2641 attacctgga aatacccgag ttgtatcctg caaggcgatg actccgtcgg cgagttctac
     2701 tcggtggcgt taacgaacaa ttatcaacaa gccgataccg gcactaaaat gatccatatt
     2761 ggcaagaaca ccaaaagcac cattgtctcc aaaggcattt cggcgggtca tggccaaaat
     2821 acctatcgcg gattggtaaa gatcatgaaa cctgctaagg gcgcgcgaaa ttattcccag
     2881 tgcgactcgt tactcctggg cgatcaatgc ggcgcgcaca cctttccata cctcgaggtg
     2941 ttcaacaata gctcgcagct ggagcacgag gcatcgacgt ctaaaatcgg cgaggatcaa
     3001 ttgttttatt gccggcaacg gggcatttcg ccagaagacg cggtcaacat gattgtcaac
     3061 ggcttctgca agcaagtgtt caaagagttg ccgatggaat ttgccgttga agcgcagaaa
     3121 cttctcggcg tgagcttgga aggaagcgtc ggctaagcga gaattttctg tgtaaagcat
     3181 tatggctcac caagtctatc tagataacaa cgcgacgaca cgggttttgc ccgaagtcgc
     3241 cgaggcgatg ctgccattcc tgaccgagca ctatggcaac ccctccagta ttcaccgctt
     3301 cggcagcggc gtgggggaac ggattgcggt ggctcgagag caggttgcca atctgatcgg
     3361 tgcggccgat ccggtcgaga ttgtttttac cagctgcggc acggagggcg acaacgcagc
     3421 gattcgggga atgctcgaag cgcggccgaa caagcgccac atcgtaacca cccaggtgga
     3481 acatcctgcg gtattgagtt tgtgccagca cttggaaaaa agaggttacc gcgtgacttg
     3541 gctcagtgtc gatgaaaacg gcgggctcga cctcgaagag cttcgctcag ctctgacgga
     3601 cgacactgcg ctggtctcgg tgatgtgggc gaacaacgag accggagtca tttttccagt
     3661 cgagcagatc ggcgcgattg tgaaagggaa gggcgttcct tttcacgtcg acgcggttca
     3721 ggccgccggc aagatccatt tgagagtcca agatctaccc gtggatctga tgaccatttc
     3781 cgggcacaag ttttatgcgc caaagggcgt cggagcgctc tacgtcaaac gcggcttgac
     3841 ctttccacct ttcataatcg gtggccatca agagcgcaat cggcgtggcg gaactgagaa
     3901 cgtcgcgagt attatcggta tgggtaaagc cgcagaggtg gcatcgagcc ggatcgattc
     3961 cgatgcggcg cggatcacga accttcggga tcaactcgaa gctctattac tcaggtcttg
     4021 tcccgggagc aggatcaacg gaagggcgca aaagcggctt ccgaacacct gcaatataag
     4081 ttttcggtat ctggaaggcg agtctatcct ggtgcttctc gatcagcagg gaatttgtgc
     4141 ctcgactggc tcggcgtgta ccgctggatc atcggagccc tctcacgttc tgcgcgcgat
     4201 gaaagttccg ccggattggc ttcaaggggc tgtccgtttt ag
//
LOCUS       DASULZ010000021         5599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_205230, whole genome shotgun sequence.
ACCESSION   DASULZ010000021 DASULZ010000000
VERSION     DASULZ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205230"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(721..1446)
                     /locus_tag="VFY96_00385"
     CDS             complement(721..1446)
                     /locus_tag="VFY96_00385"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5604937.1"
                     /translation="MVFASWRGVVGCVKPTHRPGSLEEFIRLLPEGIGVVPVYLNFQR
                     GTEDEFRAALNAVHEKVAELAREGVDLIHPEGAPPFMVHGHKGEATIVKDWEAEFKTP
                     IVTAAQTQVEALRALGIKKFVGVTYFTGSINEIFTRYFEEAGFEVVAMEGMEVAFADV
                     GRLASTEIYAHTHRAFLKNPGADGIYMLGTGWRCLDIIHLLEEDLQVPVVHPVPARVW
                     AVQKRLHVRQPVKGFGRLLEAMP"
     gene            complement(1461..2939)
                     /locus_tag="VFY96_00390"
     CDS             complement(1461..2939)
                     /locus_tag="VFY96_00390"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX5604938.1"
                     /translation="MARPERIGTSESTVTYRGLRDWIEQVDKLGELLKVNGAHWDREM
                     GSITQMLTEGGKGKAPAILFDEVPGYPKGYRTLYGQFSTIKRVALTLGLPLEYERKAD
                     IVKAYHHVMTDMKLIKPKMVKTGPIFENVLEGDRVDVFKLPVPVHHQADTHRYIGTAD
                     MCITKDPDAEWYNFGAYRCEVYDEKTIGCQITEGKHGRIHRDKYFERGQSCKFAIVCG
                     QDPLLYMLSASPLPSGVSEYDFAGGIRGEPVEVVKGPFTGFPIPADAEIVIEGEAVPG
                     QSKPEGPFGEWMGYYSDDVVPRPYLEVKTVMHRNNPILTCAPQHKPVDETGLLKGIAG
                     AAEIWNALEACGLPDILGVWNHEGAPATRFTAIQIRQRYPGHARNVLHVAANCMAGAY
                     NGKWTVVVDDDVDCSDIDQVLWAMGTRFDPITDIDLVQKAWSSGRDPMFLPGNFNNRI
                     LIDACIPYNRKLRGEFPKVVEVPDNVRAQVKAKFPQIFRSQS"
     gene            3141..3629
                     /locus_tag="VFY96_00395"
     CDS             3141..3629
                     /locus_tag="VFY96_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5604939.1"
                     /translation="MDTSTSYGQQLRSLGQCLEAQRINIFELTCRGERFLVKGEPERE
                     TSLLAALRQWQQRRRSDGLNTSLIFSRPDLDQLERQGRAQRKQSDRLPDFYSLPNALR
                     TVGQYLELKGAELLELQKRQLGLTLLSRDKDGHPQMEERSLASFYDLFLKLHDKRGKR
                     QR"
     gene            3679..4434
                     /locus_tag="VFY96_00400"
     CDS             3679..4434
                     /locus_tag="VFY96_00400"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017753413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX5604940.1"
                     /translation="MNYTLRELMVAAAAREIHDGDKVFVGMRLPLLGFAVAKEIHAPN
                     AIGVFENGVIRDWPALESIFTMSDPPNIARALSCGSLNEVMYLLQTRRIDLGFIGGAE
                     IDRFGNLNTHWVDDNGKRIRLPGSGGAADIATMARRCIIIMNHEKRRFTAKVRFNTSP
                     GFGDGGNWRERRNLIGGGPSRVISSMGIFSFDPASHEMTLSSYHPGITNEQIATETGW
                     PLRIADDVHETSPPTDEELAAVRKYDPRAVWTG"
     gene            complement(4467..4979)
                     /locus_tag="VFY96_00405"
     CDS             complement(4467..4979)
                     /locus_tag="VFY96_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5604941.1"
                     /translation="MAENNPVVSMTTSMGKIRIELDAERAPITTKNFLEYVNAGHYDG
                     LIFHRVIPGFMIQGGGMDAQMNEKKGKAPIKNEAANGLKNKIGTIAMARTNVVDSATS
                     QFFINVKDNDFLNHRSATPAEFGYAVFGQVIEGMEVLHGIEKVKTGRKGQHDDVPDTA
                     VVIQSVTLET"
     gene            5140..>5599
                     /locus_tag="VFY96_00410"
     CDS             5140..>5599
                     /locus_tag="VFY96_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX5604942.1"
                     /translation="MPSERKIEGILMPMPVAFKQDGAIDHKATDAIIDFYLDAGVHGF
                     FPLGTHGQGMVMEIDERKKVAEQIVQRVKGRVPIVMHIGTANTLSSIDLAKHAVSLGV
                     DAIGVVPPYYYPHDDWEVYAHYKAIARSIPGTPMFIYDNTETTWVEITPAK"
BASE COUNT         1278 a         1629 c         1447 g         1245 t
ORIGIN      
        1 tcctgaataa ggaatagcgg tgctgttgtc gtcgatgggt ttgttgagct tcaacgtcat
       61 gcttaaggat tcgatgcatc cgttcgtgac gtccattttg gctcggctta cccggcttaa
      121 tccgttccgg ccgaatcccc agtcgaatgc accacttcga caactcacgg attccaccga
      181 gggccaacga ggcgaagggt gcatcgttgt cagtgcggat cgcttgaggc aatccatacg
      241 tttgaaacgc ccactcaaac cagggtcgaa ccgcagcgta actcgggtgt tccaaagccc
      301 gacacaacag cagatatcgc gagaagttgt ccgacagcgt ccgcgcgtaa cagcggcggc
      361 gattacccag aacaaaatcc cctatgaaat ctgcactcca gatattggga gcttggcagt
      421 cacaaatggc tcgctatacg cactgacgtg gcgccgtttg actctgcgct tgaccaaccc
      481 ggcccgcttc aggatcgtac ccgcagtgga atcagccggc cacttcagct ctggcccatt
      541 ggcgcgcaaa tagtccaaaa tctttttcgg tccccagctc gcgcaggaaa atgtcatctc
      601 gagcagagcg agagatcttt ccgagttgtt cagcgcacga agaaagatcc ctcacattcg
      661 ttcgggatga caaaccgcaa atggaaactg tatggctttg aaaggtcagc gcgtagcggc
      721 ttacggcatt gcttccagca atcgcccgaa gcccttcaca ggttggcgca catgaagacg
      781 tttctggacc gcccacacgc gggccggcac gggatgtacg acaggaacct gcagatcttc
      841 ctcgagcaga tgaataatat cgaggcagcg ccagccggtg ccgagcatgt aaatgccgtc
      901 ggctccggga ttttttaaga aagctctatg cgtgtgcgcg tagatttccg tcgacgccag
      961 gcgccccaca tcggcaaatg ccacttccat cccttccatc gccacgactt cgaaaccggc
     1021 ttcctcgaaa tagcgtgtaa atatctcatt gatgctgccg gtgaaatagg taacgcccac
     1081 gaatttcttg atgccgagag cacgcaacgc ctcgacttgg gtttgcgccg cagtgacaat
     1141 gggcgttttg aattccgcct cccagtcttt gacgatcgtc gcctcgcctt tatgcccatg
     1201 caccatgaac ggcggcgccc cttcagggtg aatcaaatcc acgccctctc tcgctagctc
     1261 tgcgaccttc tcatggacgg cattgagcgc ggcacgaaac tcgtcttccg tcccgcgttg
     1321 gaagttcaaa taaaccggca caacgccgat gccttcaggc aaaagccgga taaattcttc
     1381 cagcgaccct ggccgatgcg tcggcttgac acaacccacc acgccgcgcc aactggcaaa
     1441 aaccataatc ggctgccgta ttatgactgt gacctgaaaa tctgcggaaa cttggccttc
     1501 acttgcgcgc gcacgttatc cggtacttcg actactttgg gaaactcgcc gcgcagtttt
     1561 cggttatagg gaatgcacgc gtcgatcaaa atgcggttat tgaaatttcc cggcagaaac
     1621 atcggatcac gtcctgacga ccacgctttt tggacgagat cgatgtcggt aattggatcg
     1681 aaacgcgtgc ccatggccca gagcacctga tcgatatccg agcagtcgac gtcatcgtcc
     1741 acgaccactg tccacttccc gttgtaagcg cccgccatgc agttggctgc gacgtgcaga
     1801 acgttgcgtg catggcctgg atagcgctgg cgaatctgga ttgcagtaaa ccgcgtggcc
     1861 ggagcgcctt cgtgattcca aacaccgaga atatccggca atccacaggc ctccagcgcg
     1921 ttccagattt ccgcggcgcc ggcaatgccc ttcaacaatc ccgtctcgtc caccggcttg
     1981 tgctgcggcg cgcaggtaag aatcgggttg tttcggtgca tgaccgtttt gacctccaag
     2041 taaggtcgcg gcaccacgtc gtcggaatag tagcccatcc actcgccaaa aggcccttcc
     2101 ggctttgact ggccgggaac cgcctcgcct tcgatgacga tttctgcatc ggccggaatg
     2161 gggaatccgg tgaacgggcc tttaacgact tccacaggct cgccgcgaat cccgccggca
     2221 aaatcatact cggagacccc cgatggcagc gggctagccg agagcatgta tagcaaggga
     2281 tcttggccgc acacgatggc gaatttgcaa gactggccac gctcaaaata tttgtcacgg
     2341 tggatgcggc cgtgcttgcc ctccgtgatt tgacagccaa tggttttttc gtcgtacacc
     2401 tcacaacgat aggcgccgaa attgtaccat tccgcgtcag gatctttggt gatgcacata
     2461 tccgccgtgc caatgtaacg atgggtatcg gcttgatggt gaaccggcac gggcaatttg
     2521 aacacgtcca ctctgtcgcc ttccaaaacg ttttcgaaga tcggtccggt cttgaccatc
     2581 ttgggcttga tcaacttcat gtcggtcatg acgtgatgat aggccttgac gatatcggcc
     2641 ttgcgctcat attcgagcgg caatccgagc gtcaaggcca cgcgcttgat cgtggaaaac
     2701 tgaccgtaga gcgttcggta acccttggga tatcccggaa cttcgtcaaa taaaatcgcg
     2761 ggcgccttgc ccttgccgcc ttcggtgagc atttgagtga tcgagcccat ttcgcgatcc
     2821 cagtgggcgc cgttgacctt gagcaattcg ccgagcttat cgacctgctc gatccaatca
     2881 cgtaggcctc ggtaggtcac cgtgctctcc gaagtgccaa ttctctcggg acgtgccatg
     2941 aatgcctccg ccgaatctat ctaacgcttc accttacgtt tagggtaata gcttgtcaac
     3001 aaagttacgc ctggcacaat cgcagagctg caattccccc cacggcacgc gtcaaagagg
     3061 tctgtcctcc gcctcggggt tgtcggaacc caacaagaat gatatgtagc gccaactaaa
     3121 tacttgtctc ggggcgcgac atggatactt caacttcgta cgggcaacag cttcgttccc
     3181 tcggtcaatg tctcgaagca caacgaatta acatcttcga gctgacctgc cgcggcgagc
     3241 gcttcctcgt caaaggtgag ccagaaaggg agacctcttt gctggccgcc cttcgtcaat
     3301 ggcagcagcg acgacgcagc gatggactga atacttcgtt gatcttcagc cggcccgatc
     3361 tcgatcaact ggagcgacaa ggacgagccc agcgtaaaca atccgatcgg cttccggatt
     3421 tttacagcct accgaacgct ctgcgtaccg tcggacaata tctcgaactg aaaggcgccg
     3481 aactcctgga actacaaaaa cgtcagctcg gtttgactct cctgtcgcgc gataaagacg
     3541 ggcatccgca aatggaagag cgcagcctcg cgtcatttta cgatcttttt ctcaaactgc
     3601 acgataaacg cggcaagcga cagagatagc gtgccggatc gctagaatcg caatctattc
     3661 caagatattc ctagtcgagt gaactacacg ctccgcgagt taatggtcgc cgcggcggcg
     3721 cgggaaatcc acgacggcga caaagttttc gtcggcatgc ggttacccct gctcggcttc
     3781 gccgtcgcca aggagatcca tgcgcccaac gctatcggtg tttttgagaa cggcgtgatc
     3841 cgcgactggc cagctctcga atccattttc accatgagcg atccgccgaa catcgcgcgc
     3901 gcgctttcat gcggcagtct caacgaggtg atgtacttat tgcaaacccg ccgcatcgat
     3961 ctaggcttta tcggcggcgc cgagatcgac cgcttcggca accttaatac ccactgggta
     4021 gacgacaatg gcaaacgaat tcgtctaccc gggagcggcg gcgccgcgga catcgcgaca
     4081 atggcacgcc gctgcatcat cattatgaat catgaaaaac gacgctttac agcaaaagtg
     4141 cgattcaaca cctcacctgg gtttggtgac gggggcaatt ggcgtgagcg gcgaaatctt
     4201 atcggcggcg gaccgagccg agtgatcagc agtatgggca ttttttcgtt cgatcccgcg
     4261 agccatgaaa tgaccctgag cagttaccat ccgggaatta ccaacgagca aatcgcaact
     4321 gaaacgggat ggccgctgcg tatcgccgat gacgtccatg aaaccagccc acccacggac
     4381 gaagagctcg ccgcagtcag gaagtatgat cctcgagcgg tttggactgg ttaatgccag
     4441 tacagccgaa gtccaatttc aacttctcag gtttcgagcg tcactgactg gatcaccaca
     4501 gcggtgtcgg gcacgtcatc gtgctgaccc ttgcgaccgg ttttgacttt ttcaattccg
     4561 tgcagtactt ccataccctc gatcacctga ccgaacaccg cataaccaaa ctccgccggc
     4621 gtagcgctac gatgatttaa aaaatcattg tccttgacat tgatgaaaaa ctgcgaggtc
     4681 gcgctgtcga cgacattcgt gcgcgccata gcgatcgtgc cgattttatt cttcaagccg
     4741 ttcgcggctt cgtttttgat cggcgctttg ccctttttct cattcatctg cgcgtccatg
     4801 ccaccgccct gaatcataaa accgggaatc acgcgatgaa aaatcagccc atcgtagtgt
     4861 ccagcgttga cgtattccaa aaagtttttg gtcgtgatcg gtgctcgttc ggcgtcgagc
     4921 tcaatgcgta tcttgcccat gctcgtcgtc atcgatacca ctgggttatt ttccgccatt
     4981 cctgccctcc tttagatacg gtttatgcat aacatctttt tccggtaagc gacatcgagc
     5041 agcgcttttc atagcttctc tttgtcggtg gaattatctt tgaggttgcg ctatggatac
     5101 cctagctaaa ccaggcgcta gagagaaagg aggcacccaa tgccgagcga gagaaaaata
     5161 gaaggtatct tgatgcccat gccggttgct ttcaaacagg acggagccat cgatcacaaa
     5221 gccacagatg cgatcatcga tttttacttg gatgcgggtg tgcatggctt ttttccgctc
     5281 ggcacgcatg gccaaggcat ggtcatggaa atcgacgaac gaaaaaaagt cgcggaacaa
     5341 atcgtccaac gcgtgaaggg ccgcgtgccg atagtgatgc atatcggcac agccaataca
     5401 ctgtcttcca tcgacctcgc caagcatgcg gtcagcctgg gagtcgatgc gatcggcgtc
     5461 gtgccacctt attattaccc acacgacgac tgggaagttt acgcgcacta caaggcgatt
     5521 gcccgctcca tacccggaac gccgatgttc atctacgaca atacggaaac cacctgggtt
     5581 gaaatcacgc cggctaaag
//
LOCUS       DASULZ010000022         4494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_205371, whole genome shotgun sequence.
ACCESSION   DASULZ010000022 DASULZ010000000
VERSION     DASULZ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205371"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VFY96_00415"
     CDS             complement(<1..527)
                     /locus_tag="VFY96_00415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX5604943.1"
                     /translation="MAVKFGWKAGTEQYPPLELLEYAAAAEEASFDSIDVSDHFHPWS
                     EHGQACFVWTWLGAAAVRTRKIMLGTGVTCPILRYHPAIIAQAAATLAAFAPGRSFLG
                     LGTGEALNEYSATGLWPSYQTRQAQLVEAVQLIRALWKGDPVSYSGTYYKTRKAKLYT
                     RAETSPPILISALVP"
     gene            complement(549..1115)
                     /locus_tag="VFY96_00420"
     CDS             complement(549..1115)
                     /locus_tag="VFY96_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEX5604944.1"
                     /translation="MADSLSGMKIAILVTNGFEQSELTEPKKALEQAGAQLFIISPAG
                     KQVRGWNHTEWGDSFPVDVALDDVQPADYSALLLPGGVMNPDYLRWNRRAVTFVKSFV
                     DANKPIAAICHGPWTLIEAGAVKNRKITSWPSLRTDLINAGAQWVDQEVVVDGRLVTS
                     RKPDDIPAFNQKMIELFAQKEEPHRAIG"
     gene            1345..3093
                     /locus_tag="VFY96_00425"
     CDS             1345..3093
                     /locus_tag="VFY96_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016951982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5604945.1"
                     /translation="MADTASDVLMDILIDWNVEVIFGLPGDGINGLMEALRKRKDKIR
                     FIQVRHEEAAAFMACAYAKYTGRLGVCLATSGPGGIHLLNGLYDAKLDGQPVLAITGH
                     HYHDLIGTHAQQDVDLDKLFMDVAVYNTRIMGLAHVEPVTHLACRTALSYRGVAHINF
                     PTDIQDFTSAERSKRDIPGHTSAVISRGAGAPDDRDLQYAADILNRGKKVAILAGRGA
                     LGAGEELEETAEILGAPIVKALLGKAAVPDDSPYTTGGIGLLGTRASQEALERCDTLL
                     LVGTSFPYIEFMPKPGQAKAVQIDIDPTRIGLRYPVDVGLVADVCIALKALGPKLTRH
                     EDRGFLRQAQEAMSEWWALMQERGTRTDHPMKPQVVAWELGKRIAPDAIVSSDSGTIT
                     TWFARQIPAKRNQMFSLSGTLATMANGLPYAIAAQVAYPERQCIAFVGDGGFSMLMAE
                     FATAVKYQLPIKVIVIKNNTLGQIKWEQMVFLGNPEYGCELQPIDFAMFARACGGSGF
                     TIGKASECGAVLDGALSTPGPVLIQAEVDANEPPMPAKITMEQAEKFAESLIRGTPHR
                     GDIALTVLSDKVRELV"
     gene            complement(3175..3891)
                     /locus_tag="VFY96_00430"
     CDS             complement(3175..3891)
                     /locus_tag="VFY96_00430"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEX5604946.1"
                     /translation="MFEDKVDREELEKMPSEYKELLERVLTIQADCEIGGPHLYVKDV
                     LPSAPSKINQLIVARTAAEEMDHYRKFARLAGEIGKDTSFLLSIPNQKRYLEAFRGMI
                     TTWEDFSVFGFLIDRVGRYQLEEFLDCSYLPLQRILPEVLKEEMGHIGFGTNQTAELA
                     AKNDESRERVQQSLNFWYVKGLDMFGRSDSERSNRFCHWGLKRRSNSQARWEFKKEVD
                     PIIQSMGLTVPDPVEGRKFL"
     gene            complement(3929..>4494)
                     /locus_tag="VFY96_00435"
     CDS             complement(3929..>4494)
                     /locus_tag="VFY96_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5604947.1"
                     /translation="LAAGAVISTFLLPGVGPILAVGLGSAIFGVGGAVAGGAVGNFLE
                     ESLTRGVPKDEIFLYEDALRQGRTVVIAFADDEERIERGRGIMERAGAESLDAARDSW
                     WIGLRDAEAAEYDPDGRSFGEAEQTYRSGFEAALRPDCRGKSLEETRAVLRDRYAEVC
                     DQDTFRRGYERGQRYYAQSNRHSLRGE"
BASE COUNT          987 a         1312 c         1196 g          999 t
ORIGIN      
        1 ctaggaacca gcgccgagat caagatcggc ggagaagtct ccgcacgcgt gtaaagcttt
       61 gctttccgtg tcttgtagta ggttccactg tatgaaactg gatcgccttt ccatagagcg
      121 cgaatcaatt gaaccgcttc gaccagctgc gcctgccgag tttggtaact tggccacagc
      181 ccggtagctg aatactcatt gagggcttct ccagtcccca agccgaggaa cgagcgtcct
      241 ggtgcgaagg ccgccaatgt ggcagcagcc tgagctatga tcgccgggtg ataacggaga
      301 atcggacacg tcactcccgt gccaagcatg atcttgcggg tacgaacggc agctgcgccg
      361 agccatgtcc aaacaaaaca ggcctggcca tgttcgctcc acggatgaaa atggtcgctc
      421 acgtcaatgg aatcgaagct cgcttcttcc gctgccgctg catattccag caattctagc
      481 ggcggatatt gctcggtccc cgctttccaa ccgaatttaa cggccatcgt ctttgctcct
      541 tgccggcttc agccgattgc gcgatgcggc tcttctttct gcgcgaacag ttcgatcatt
      601 ttttgattga aagctggtat gtcgtccggt ttgcgactgg tgaccaggcg accgtcgacg
      661 acgacctctt gatccaccca ctgcgccccc gcgtttataa gatcggtccg caatgatggc
      721 cacgaggtga tcttgcgatt tttcaccgcc ccagcctcaa tcaatgtcca cggcccatgg
      781 cagatcgccg cgatcggctt attggcgtcg acgaacgact tcacgaacgt gactgcgcgc
      841 cggttccagc ggagataatc cggattcatg acgccgcccg gcaaaagcaa cgcggaatag
      901 tcggcgggtt gcacgtcatc taacgccaca tcgactggaa agctgtcgcc ccactccgta
      961 tggttccagc cccgcacttg ctttcccgcc ggtgaaatga tgaacagctg cgcgcccgct
     1021 tgttcaagag cctttttcgg ttccgtcaat tctgattgtt caaaaccgtt ggtaacgaga
     1081 atcgcgatct tcattcctga caatgaatcg gccattgtga acctccaccg agtttcatcg
     1141 aaagtggcgc aagcgttatg ccatgccgct cggcagagat gcattcccgt ctcattccgg
     1201 agatctcgac cagtcgtccc attaccagat cattttcaag cgggcgtgtt gcgatttcag
     1261 tccagcagcg acgctggacg gtggcatctc gattgctcga taagattacc caaaagaact
     1321 cttctctcag taaaggacga gaatatggct gatactgcat ctgatgtttt gatggatatt
     1381 ctcatcgact ggaatgtcga agttatattc ggactaccag gcgacggcat caacggctta
     1441 atggaagcgc tgcgcaagcg aaaagacaaa attcgtttca ttcaagtgcg gcatgaagaa
     1501 gcagccgcat tcatggcgtg tgcttacgcc aagtatacgg gtcgactggg cgtctgcttg
     1561 gccacctcgg gtcctggtgg aattcacctc ttgaatggat tgtatgacgc caagcttgat
     1621 ggccaaccag tgttggcgat caccggtcat cattatcatg acctgatcgg tacgcatgcg
     1681 cagcaagacg tcgatctcga caagcttttt atggacgtcg ccgtgtacaa cacgcgcatc
     1741 atgggattgg cgcacgtgga accggtgacg catctggcct gccgtaccgc gctcagctac
     1801 cgcggcgtag cgcacattaa ctttccgacg gatatacaag acttcacatc tgcagaacgg
     1861 tcgaaacgcg atatacccgg tcataccagc gcagtcatat cgcgcggcgc tggcgcgccg
     1921 gatgacagag atctgcaata cgccgcggat attctcaatc gcggaaaaaa agtggcgatc
     1981 ctcgcgggtc gtggcgcgct cggcgcgggt gaagagctgg aagagaccgc tgaaattctt
     2041 ggtgcgccga ttgtcaaagc attgctcggc aaagctgctg tccctgacga cagcccttac
     2101 acgaccggcg gcattggcct actcggaacc cgcgcctctc aagaagcgtt agaaagatgc
     2161 gatacgcttc tgctcgtggg gacgtctttc ccttacatcg agttcatgcc gaaacccggg
     2221 caagcaaaag cggtgcagat cgatatcgac ccgacgcgga tcgggttgcg ctaccccgtc
     2281 gatgtcggac tcgtcgctga cgtttgcatc gcattgaaag cgctcgggcc aaaattgacc
     2341 cgtcacgagg atcgcggttt tctccgccag gctcaggaag ccatgagcga gtggtgggct
     2401 ctcatgcagg agcgcggcac tcgcacggat catcctatga agccgcaggt ggttgcctgg
     2461 gagctcggca aaagaattgc ccccgacgcg attgtgtcat cggactcggg aacgattacg
     2521 acctggttcg ctcgacagat tcccgccaag cgaaatcaaa tgttttcgct ttctggaacg
     2581 ttagccacca tggccaacgg tttaccttac gcgatcgcgg cgcaggtcgc ttatccagag
     2641 cggcagtgca tcgcatttgt cggcgatggc ggcttctcca tgctgatggc cgaattcgcc
     2701 actgccgtta aatatcaact gccgatcaaa gtgatcgtta ttaaaaacaa tacgctaggc
     2761 caaataaaat gggagcagat ggtcttcttg ggcaaccctg agtacggctg tgagctgcag
     2821 ccgatcgatt ttgcgatgtt cgcacgggcc tgcggtggtt cgggctttac cattggaaaa
     2881 gcgtccgaat gcggcgctgt cctcgatggc gcattatcga cccccgggcc ggttttgatc
     2941 caggccgagg tagacgccaa cgaacccccc atgccggcga agatcaccat ggaacaggcc
     3001 gaaaaatttg ccgaatcgtt gatccgcgga acgccgcatc gcggtgacat cgccctcaca
     3061 gtcctgagcg ataaggtgag agagctggta taaagaaccg gcctcaaatg gggtctgccg
     3121 cgacggcgac taacggcgag cacggcatcg atccagcgct ttccgacgca atctctagag
     3181 gaattttcgg ccttcaacgg gatctggaac cgtcagaccc atgctctgaa taatcgggtc
     3241 cacttccttc ttgaactccc agcgtgcttg gctgttgctg cgccgcttca acccccaatg
     3301 gcagaaacgg ttcgagcgtt cggagtccga acggccgaac atatccaacc ctttcacgta
     3361 ccaaaaattc aggctctgct gcacacgctc gcgcgattcg tcgtttttcg ccgccagctc
     3421 cgctgtttgg ttggtgccga acccgatatg ccccatctcc tctttgagca cctcgggcaa
     3481 aatacgttgc agcggcaggt agctgcagtc gagaaattct tccagttgat accgccccac
     3541 gcgatcgatc aaaaaaccga aaaccgagaa atcttcccac gtcgtgatca tgccgcgaaa
     3601 ggcttcaaga taacgcttct gatttggaat actcaataga aatgaagtgt ctttaccgat
     3661 ttcgccggcc agccgggcga atttgcggta gtgatccatt tcttccgcgg cggtgcgcgc
     3721 gacgatcaac tgatttatct tgctaggcgc cgagggcaaa acgtctttga cgtagagatg
     3781 cggtccgccg atttcacaat cggcttggat ggtcagcacc cgctccagca gttccttata
     3841 ctcggatggc attttttcta actcttcgcg gtcaacttta tcttcaaaca ttgagactat
     3901 tttcctccgc aaataggata gtcaatgact attcaccgcg cagactgtgg cgattcgatt
     3961 gtgcataata acgctgtccg cgctcatagc ctcggcggaa agtgtcttga tcgcacacct
     4021 cagcatagcg gtcgcgcaac acagcgcgag tttcctctag cgatttacca cggcagtcgg
     4081 gccggagcgc ggcttcgaac ccggagcgat aggtttgctc cgcctcaccg aaggatctac
     4141 cgtcggggtc atattctgcc gcttctgcat cacgcagtcc gatccaccag ctgtctcgcg
     4201 cggcatctaa actttccgcg ccggctcgct ccatgatgcc gcggccgcgt tctatgcgct
     4261 cctcatcgtc ggcaaacgca attaccacgg tccgtccctg tcgaagtgcg tcctcgtaaa
     4321 gaaaaatctc gtctttgggt acgccgcgag tcaagctctc ttccaaaaaa tttcccacag
     4381 cgccgccggc taccgcgcca ccgacaccga agatggccga gcccagccca acagcgagga
     4441 tcggacccac tcccggaagc agaaaggtgc tgatcactgc tcctgcggct aaac
//
LOCUS       DASULZ010000023         2503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_408427, whole genome shotgun sequence.
ACCESSION   DASULZ010000023 DASULZ010000000
VERSION     DASULZ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408427"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VFY96_00440"
     CDS             <1..790
                     /locus_tag="VFY96_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5604948.1"
                     /translation="PTERHAIAWLGNGVVGMEIDAQLFFLPFESSRRTLEVATHILTL
                     LTPAITQYRRDRIIALPEWVDDFHFKSEEPLYLEINDLLGKLNRLESELASWKDYKAI
                     LASSGIYLRNKLVALFESVFGLKVQCMDREERFLVVKDDDSPMALVESEGTQGPVQMA
                     SIERLARARRQHSLPDSFPATLLINNDMAITNVSERSATSIGSDVLEASVRQNVLVVR
                     TIDLLFLLNHLEKSPNKKGRMLDLLTSGGGWLKASTGSYDIIHS"
     gene            complement(927..2396)
                     /locus_tag="VFY96_00445"
     CDS             complement(927..2396)
                     /locus_tag="VFY96_00445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEX5604949.1"
                     /translation="MASLRLLRIGAAFCAINLLMTIGGWAAERQAVQANRAQGLPEFT
                     GLAERLAPVVVNVSTRAKVSREQAPTAPFGENDPLAEFWRRFFGSPPGGGRGGSVQEH
                     LGSGFIVDRSGVVLTNNHVIEGADKITVKLTDEREFDAKVVGRDPKTDIAVIRIGDGK
                     ENFPVAPLGDSSQLQVGEWVAAMGSPFGLANTITAGIISAKGRNIGAGPYDSFIQTDA
                     SINPGNSGGPLVNLRGEVIGINSAILSQTGGNLGIGFAIPINLAKEILPELIKTGKVR
                     RGWLGVSIQRVTPEIAKAMGLERDRGALVSEVIQGSPAEKAGIRSGDVITEYDGKSIE
                     HANDLPILVAQTPVGKNVSVVVQRENKRMPVDVTVGELKEEEKVASVPEKARLGLSVQ
                     TVTPDIARSLGLEQAQGVIVSSVQPGSPAAEAGLLRGDVILEVNRGPIKSAAELQKII
                     DSAKAGANLLFLVRRSGNNLFLAMNVPEKDGETGSQPRG"
BASE COUNT          535 a          754 c          642 g          572 t
ORIGIN      
        1 accgacggaa cggcacgcca ttgcctggct gggcaacggt gtggtcggaa tggaaatcga
       61 tgcacaacta ttttttctgc cctttgaaag ctctcgacga acactcgaag tcgccacaca
      121 catcctgacc ttgttgacac ccgctattac ccagtatcgc cgcgaccgca tcatcgccct
      181 gcccgaatgg gtcgatgatt ttcattttaa atcggaggaa ccgctctatc tggaaatcaa
      241 cgatctgctc ggaaaactca accgcctcga gagtgagctc gcctcatgga aagactacaa
      301 agcgattttg gccagctcgg gaatttatct gagaaataag ctcgtggctc ttttcgagag
      361 tgtctttggg ttgaaggtac aatgcatgga tcgcgaagag cgtttcttag tggtcaaaga
      421 cgacgactca ccgatggctc ttgtggagtc cgaagggacc cagggcccgg tacaaatggc
      481 gtccatcgag cggctggcgc gtgcccggag gcagcacagt ctgcccgatt cctttccagc
      541 cacattgttg atcaacaacg acatggcgat cactaatgtc agcgaaagat cagcaactag
      601 catcggctcg gacgttctcg aagcctcagt gcggcaaaac gtgctcgtcg tacgcaccat
      661 cgatctgcta tttttgctca atcatctcga aaagagcccg aacaaaaaag gccgcatgct
      721 cgatctcttg acttcgggcg gcggttggct gaaagcaagc acggggtcat acgacataat
      781 tcactcttag ccttgctcgc tgcggcgcgg cgaaatctcg gatcgtagat ccggctcgcc
      841 cgctgtactt cgtctcgaca gggtgcttta cgcgctcatc cgccggaaaa gcccagcgaa
      901 acacaccttg aaaacgggat tgggaattat cctcttggtt gagatccggt ctcgccgtct
      961 ttttccggca cgttcatagc gagaaaaaga ttgttgccgc tgcgccgcac caggaacaat
     1021 agattggctc cggcctttgc gctatcgata attttttgca gttccgcggc gctcttgatc
     1081 ggcccccggt tgacctcgag gatgacatcg ccgcgcaaca aaccggcctc cgccgcggga
     1141 cttccaggct gcacgctact aacaatcacg ccctgggctt gttcgagacc caagctgcgc
     1201 gcgatatcgg gagtgacagt ttgcacggag agacccaatc tcgccttctc cggcacggaa
     1261 gcaacttttt cctcttcttt gagctcgccg acggtcacgt caacgggcat gcgtttgttt
     1321 tcgcgctgaa cgaccacgct gacgtttttt cccaccggtg tttgcgccac caggatcggt
     1381 aagtcgttcg cgtgctcgat gctcttgccg tcgtattccg taatcacgtc ccccgatcgg
     1441 attccggctt tttccgccgg gctcccttga attacttccg agacgagagc gccacgatcc
     1501 cgttccaatc ccatcgcttt cgcgatctcg ggggtcacgc gctgaatact cacgcctaac
     1561 cagccgcgtc tgacttttcc cgtcttaatc agctcaggca gaatctcctt cgccaaatta
     1621 atcgggatgg caaatccaat accgagatta ccaccggtct gactcagtat tgcgctattg
     1681 atgccgatca cctcaccgcg aagattgacg agcggcccgc cggaattgcc gggattgatg
     1741 gacgcatcgg tctggataaa gctgtcatag ggtcccgcac caatatttct ccccttcgca
     1801 ctgatgatac ctgccgtaat ggtattggcc agtccgaacg gacttcccat ggcggcgacc
     1861 cactcgccca cttgaagttg gctcgaatcg cctaacggcg ccaccggaaa attttctttg
     1921 ccgtcgccga tgcgaattac ggcgatgtcg gttttgggat cgcgtccgac gaccttcgca
     1981 tcgaattcgc gttcgtcggt cagcttgact gtgatcttgt ccgccccctc gatgacatgg
     2041 ttattcgtca aaaccacgcc gcttcggtcg acaataaatc ccgaccccaa atgttcctgg
     2101 actgaaccgc ctcgcccgcc tccaggcggg ctgccgaaaa atcttcgcca aaattcggcg
     2161 aggggatcgt tttcgccgaa cggcgcggta ggcgcttgct cacggctgac tttcgctcgc
     2221 gtcgaaacgt tgaccaccac tggggcaagg cgttccgcga gaccggtaaa ctcgggcaga
     2281 ccttgggcac gatttgcctg tacggcctgc cgctcggcgg cccatccccc aatcgtcatc
     2341 aacagattga tggcacaaaa tgccgccccg atacgcaaaa gacgtaaaga cgccattcgg
     2401 ttttctccta ttcgcgatct catccaatgg gctaggtggc aacggacatg ccaagcgaaa
     2461 tatccgcgct atcggagcat ttgctaaact gtattcgcac tga
//
LOCUS       DASULZ010000024         5647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_205738, whole genome shotgun sequence.
ACCESSION   DASULZ010000024 DASULZ010000000
VERSION     DASULZ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5647
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205738"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VFY96_00450"
     CDS             complement(<1..610)
                     /locus_tag="VFY96_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5604950.1"
                     /translation="MAKPKTRLNSQTSALAKTVYSTLEMPESQSTSVANSTNGWWDSR
                     LLRWFVPSALFLGSLLIVIITAGDYGVVWDEPAYFHASDLHVGWLKRLLHGPHDDFSA
                     LLDDNVIAEAWHWNPYNVPHPPLSRIISGLTHILFYPFLDKFISYRVAPGLFFAALVT
                     VAYLWVKELFGKLAGLFSAMAILLMPNLFAFAHMAVTDLPLAT"
     gene            complement(586..1308)
                     /locus_tag="VFY96_00455"
     CDS             complement(586..1308)
                     /locus_tag="VFY96_00455"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5604951.1"
                     /translation="MSKSREFSYPGAELSLFAQASNWKSYVKSQIDEYLIGDVLEIGA
                     GIGGTTIALNDGSARRWFCLEPDQNQVNRLQLLLGENRGHAPLVVAGSLAAFAQKPTF
                     DCILYIDVLEHIDDDRLQIQTAARLVRPGGHIVVLSPAHEWLFSEFDKNIGHRRRYNK
                     RSLGKLMPSEWKEIKVKYLDSIGVFLSLGNVIALRQSLPTSSQLELWDRICVPVSRAF
                     DRLLLGTFGKSVLAVWQNPRRG"
     gene            complement(1292..2278)
                     /locus_tag="VFY96_00460"
     CDS             complement(1292..2278)
                     /locus_tag="VFY96_00460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5604952.1"
                     /translation="MFEFAPAANQLVILMPVYNDWQALSLLLPRLDQELAENGLNAQV
                     FLVDDGSTLLPSENLFSLGYESIQSIDVLALRRNLGHQRAIAAGLSYIEANVGCQYVV
                     IMDGDGEDDPSDVPKLLQKCLASGNKIVFAARTRRSEGWLFRFLYQMYRLVHFALTGV
                     KVQVGNFSVVPVIALKKLVAVSELWNHYAAAVHKAKLPLDVVPTARGCRLAGPSQMDI
                     VALVVHGLSAMAVFGDRIGVRLLLLVFLGILLDLGALLSVIAVRLVTSWAIPGWATYA
                     TGILLIILIQMSVVVLVFVFVILASRDRINFIPARDYVHLAGAVQRVYEQVA"
     gene            complement(2744..2992)
                     /locus_tag="VFY96_00465"
     CDS             complement(2744..2992)
                     /locus_tag="VFY96_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5604953.1"
                     /translation="MHDSLQQLSNVALAADYSEVALKLLTDSRYDAPIQIQKILIPDV
                     PPRNPDHQSRAVVASIGGLGCRCHNGRGYSCSSALDAR"
     gene            complement(3095..3514)
                     /locus_tag="VFY96_00470"
     CDS             complement(3095..3514)
                     /locus_tag="VFY96_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5604954.1"
                     /translation="MNKLIAIVLPTVFLAFFPAAVLSQGIFEYGRAVGSVPHRQGITG
                     GKVSGGAQQGGGTVGGMGDVGGRAMPTRLVVSAKTTGLYPRQDEETEKLEQLSQGDAL
                     SPMVQTAGTNSWYMVKTSKGLVGWVKGTDVREEITKK"
     gene            3862..4638
                     /gene="kdsB"
                     /locus_tag="VFY96_00475"
     CDS             3862..4638
                     /gene="kdsB"
                     /locus_tag="VFY96_00475"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEX5604955.1"
                     /translation="MSVVAVIPARYGSTRLPGKPLAMLGGKPMIQHVYENASKAKVLD
                     EVIVATDDRRIEQAVKNFGGNVVTTSKNHASGTDRMAEVARKFKSADWLVNIQGDLPF
                     IRARTIARAIRPLMGDRKIPMGTVCTAIYDMNEWNNPNVVKVLKDRAGFALYFSRAPI
                     PFFRNSRIDLAGKSSTSSSNQRLWGYRHLGLYVYRRDFLLKFAVLRRTILEQIESLEQ
                     LRALQNGYPIYVAEVDEHPIEVDTPDDLKKAEYYLKNRIH"
     gene            4648..>5647
                     /locus_tag="VFY96_00480"
     CDS             4648..>5647
                     /locus_tag="VFY96_00480"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX5604956.1"
                     /translation="MATVKTKFIFVTGGVVSSLGKGLAAASIGALLESRGLKVTMLKM
                     DPYINVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERYISTRMGKKNNCTTGQIYETV
                     IAKERRGDYLGGTVQVIPHITDEIKRRILAAAEGYDIAIGEVGGTVGDIEGLPFLEAV
                     RQIAWDWGRENVLYVHLTLVPYITGGGELKTKPTQHSVKELTGLGIQPDILLCRTDRI
                     LDRKVKTKIAHFCNVAENAVITAKDVEWIYEVPLVFHEEGLDERVVEKLNIWTGAPHL
                     ENWQRVVHVLKNPLDSVRIAIVGKYMSLKESYKSLLEALVHGGIANEVRVDLDCIEA"
BASE COUNT         1492 a         1428 c         1447 g         1280 t
ORIGIN      
        1 aagtcgccaa tggcaaatcc gtgaccgcca tatgagcaaa tgcaaataga tttggcatca
       61 acaggatggc cattgctgaa aatagtccgg cgagttttcc aaaaagctct ttgacccaga
      121 gatatgccac cgtcacaaga gcagcgaaaa acagaccggg tgccactcga taggagataa
      181 atttgtccaa aaaggggtag aacagtatgt gtgtaagccc agaaatgatc cgggaaagcg
      241 ggggatgtgg aacgttgtac ggattccaat gccatgcttc tgcgatgacg ttatcatcca
      301 gcaacgcaga gaaatcgtca tgcggcccat ggagcaacct tttgagccaa ccgacatgca
      361 aatccgaagc gtggaaatat gctggttcgt cccagaccac gccataatca ccggctgtga
      421 taatgacgat taaaagcgac cccaaaaaaa gtgcactggg aacaaaccaa cgcagcaatc
      481 gtgaatccca ccagccgttg gtactgtttg ctacgctggt cgactgtgac tcgggcattt
      541 ccagggtaga ataaactgtc ttggctaacg cagatgtttg gctattcaac cgcgtcttgg
      601 gttttgccat acggccaaca ctgacttccc gaaagtaccc agtaaaagcc tgtcgaaggc
      661 tcgtgaaacc ggaacacaga tacgatccca cagttctaac tgagacgaag taggaagcga
      721 ttgccgcagg gcaattacat ttcccaaaga gaggaaaacc ccgatgctgt caagatactt
      781 gacctttatt tctttccatt ccgacggcat gagcttgcct agactccgct tgttatagcg
      841 tcggcgatgt ccaatgtttt tgtcaaattc actaaaaagc cattcatgtg cgggagacaa
      901 caccacaata tgacctccag gccgtacgag ccgagccgcc gtctgaattt gcaatcggtc
      961 gtcgtcaata tgttccagaa catcgatgta taaaatacaa tcgaaggtcg gtttttgggc
     1021 gaaggcagca agggaacccg cgacgacaag cggcgcgtga ccccgatttt cccccagaag
     1081 caactgtaga cggtttactt gattttgatc cggttccagg cagaaccagc ggcgggccga
     1141 tccgtcgttt agtgcgatcg tcgtgccgcc tatcccggcg ccgatctcaa gcacatcgcc
     1201 aataagatat tcatcgatct gagacttgac atacgatttc caattgctcg cttgcgcaaa
     1261 cagagagagc tcggcgcccg gataggaaaa ctcacgcgac ttgctcataa acccgctgaa
     1321 cagcgccggc caaatgaaca tagtcccggg cgggaataaa attgatccga tcgcgggacg
     1381 ccaaaatcac gaagacgaaa accaaaacca caactgacat ttgaatcaaa attattaata
     1441 ggattccggt tgcataggtt gcccaaccgg gaattgccca ggacgtaact aagcggacag
     1501 caattacgct cagcaaagct ccaagatcga gcaggattcc gagaaacaca agcagcaaaa
     1561 gccgaacgcc aatgcgatca ccaaaaacgg ccattgcgct gagtccgtgc acgacaagtg
     1621 caacgatgtc catttgtgaa ggtccggcca gtcgacagcc gcgggctgtc ggaacgacat
     1681 ccagaggaag tttcgcttta tgcacggccg ctgcatagtg gttccacagc tcggatactg
     1741 cgactaactt tttcagggca attaccggta cgacgctaaa gttgccgact tggactttga
     1801 cgccggtcaa cgcaaaatga acaagccggt acatctgata aagaaaccta aagagccaac
     1861 cttctgagcg acgcgtccgc gcagcgaaga caattttatt cccgctcgca aggcattttt
     1921 gaagcagctt aggaacatca ctagggtcat cttctccgtc gccatccata ataaccacgt
     1981 actggcaacc cacattcgcc tcgatgtatg acaatccggc tgctattgca cgctgatgtc
     2041 caagattgcg acggagggcg agtacgtcaa tgctttgaat gctttcgtaa cccaacgaaa
     2101 acaaattttc cgatggtaat agggtcgatc catcatcgac caaaaaaact tgcgcgttga
     2161 gaccgttttc agctaattcc tggtccaatc ggggcaacag tagtgaaagg gcctgccaat
     2221 cattgtacac aggcattaaa atgactagct gattggctgc aggcgcaaac tcaaacactc
     2281 gaatgaaccc tcgagatctt gaaaccggcc gctatcctaa tcgatacgat tgggttcttc
     2341 ttgctccgat aagggccgac gccgaaagca atgagtgaat caaaatcggc cctagaatac
     2401 cacatcgtcg gaatagcaaa aagtaaactt aatgatcgcg ggctggcgcg gtagttttgt
     2461 tccggcgagg agctcgtaaa ctcttaacgt cagttctgat ctagcttcgt tgaagtcata
     2521 ggattgcctc taatagctaa ttgattagcc aggtcggagg ggtcgaactg tctttaacag
     2581 ttgcagtcga aactcactac acgatcgtcg cattcagtag cgtctggcgg agagaattga
     2641 tgtagtttgt ttgttctaaa cggagattag ccgatcaaca gcagaaggat tacgaagccg
     2701 ggcgaattgt ttggctgcga gacacgctca ctttcaacag gaatcatcgc gcatcaagcg
     2761 ccgaagaaca ggaatagccg cggccattgt gacagcggca cccgagaccg ccgatgctgg
     2821 cgacgacagc ccttgattgg tggtctggat tacgcggggg aacgtcggga ataagtatct
     2881 tttggatttg aatcggtgca tcatatcggc tatccgtcag cagcttcagc gccacctcgg
     2941 aatagtcggc cgccagagcg acattggata attgctggag gctgtcgtgc aaacagcaag
     3001 caaattggtg aaacccttac agggggaatc agccaatttc ttgcttcgag tcttcgagca
     3061 gacacttctg cctcgaccat ggcgatggca aggctcattt cttggtaatt tcttcccgaa
     3121 cgtcggttcc ctttacccaa ccgactaggc ctttagaagt tttaaccatg taccacgaat
     3181 tagtgcctgc ggtttgtacc atcggtgaaa gcgcatcgcc ttgtgacaac tgctctagct
     3241 tctcagtttc ttcgtcttga cgcgggtaaa gcccagtggt ctttgccgaa accaccaaac
     3301 gggtcggcat cgcgcgaccg cctacgtcac ccatcccgcc gaccgtacca cctccttgtt
     3361 gagcgcctcc ggacactttg ccacctgtga ttccttgcct atgcgggacg ctgccaacag
     3421 ctcggccata ctcaaagatt ccctgagaca aaacagcagc gggaaagaag gctaagaata
     3481 cagtcggcaa aacaatcgcg atgagtttat tcattgtgaa catatcctcc aatcttctgc
     3541 cgcatcatat aacgcaaggt taggggcaac aacatttttc tcgctaatgg taagcttgcg
     3601 gcgaagtcgt tacagcgatt ggatagcgtg actgtatgat caaagctgtt ttgcaaccgc
     3661 tgctgactcg caaattgtcg gaattcctgg cacggtcacg tacccgcccg atcacgtaaa
     3721 tctcgttgac acgaattcga tgcccacgta tattgaaagc ccgtggtgcg cttttcgtga
     3781 agccatcacg gtttttcttt ttcagtaata ccgcgaatct gtttggtgtg atcaaatcaa
     3841 ctattttcca agcctgagcc aatgagcgtg gtcgctgtga tcccggcgcg ttacggctcg
     3901 acgcgattac caggcaagcc gttggcgatg ctcggcggca agcccatgat ccagcacgtt
     3961 tatgaaaatg cttccaaggc aaaggtgctg gatgaagtga tcgtagccac tgacgatcgc
     4021 cgaatagagc aagccgtcaa aaattttggc ggcaatgttg tgacgacctc gaaaaatcac
     4081 gccagcggca ccgatcggat ggcggaagtc gcacggaagt tcaaatcagc ggactggctc
     4141 gtcaatattc agggtgatct gcctttcatt cgcgcgcgaa cgattgcgcg agccatcagg
     4201 cctttgatgg gtgatcggaa aattcccatg gggaccgtat gcacggcgat ctatgacatg
     4261 aacgagtgga acaatcccaa cgtagttaaa gtgctcaagg atcgcgcggg cttcgccctt
     4321 tatttttcgc gcgcaccgat tccttttttt cgaaatagcc ggatcgacct ggccggcaaa
     4381 agtagcacgt cttcatccaa tcagcgcttg tggggctatc ggcatctggg cttgtatgtt
     4441 taccggcgtg atttcctgct gaaatttgct gttctgcggc gaacaatcct agagcaaatt
     4501 gaaagcttgg agcagcttcg agcactccag aacgggtatc caatttacgt tgcagaagtg
     4561 gatgaacatc cgattgaagt ggatacaccg gatgatctca agaaagccga atactatttg
     4621 aaaaatagga tccactgaaa ggaagccatg gcgactgtca aaaccaagtt tatttttgtc
     4681 accggcggcg tcgtttcttc tttgggtaag gggctcgcgg cagcctcgat cggcgcactt
     4741 ttagagagcc gggggctcaa ggtaaccatg ttaaaaatgg atccttatat taatgtcgat
     4801 ccaggtacga tgagtccgta ccagcacggt gaagtctttg tcacggacga cggcgcggaa
     4861 accgatttag atctcggcca ttacgagcgc tacatttcaa cccgcatggg aaaaaagaat
     4921 aactgcacga ctggccaaat ttacgagact gtaatcgcca aggagcgccg cggcgattat
     4981 ctaggcggca cggtgcaagt aattcctcac atcaccgacg aaattaagcg gcgtattctt
     5041 gccgcggcgg aaggttacga tatcgcgatc ggcgaagtcg gcggcacggt gggtgatatc
     5101 gaaggattac cgtttttaga ggcggtccgt cagatcgcct gggattgggg ccgtgaaaat
     5161 gttctctatg ttcatttgac cttggtgcct tacatcaccg gcggcggcga attgaagacc
     5221 aaaccgacgc agcatagcgt taaggagttg acaggcttag gaatccagcc tgacatcttg
     5281 ctctgtcgca ccgatcgcat tctcgacagg aaagtaaaaa ccaaaatagc gcatttctgt
     5341 aatgtcgcag agaatgcggt catcaccgcg aaggatgtcg aatggatcta tgaagtgccg
     5401 ctggttttcc acgaagaagg gctcgatgag cgggttgtcg agaagctcaa tatctggacc
     5461 ggcgcgccgc atctggaaaa ttggcaacgg gtggtgcacg tgcttaaaaa tccgctcgat
     5521 tccgtccgca ttgcgattgt cggcaagtat atgagcctta aagaatccta caaaagcttg
     5581 ctcgaggctc tggtgcacgg tggaatcgcc aatgaagttc gggtcgatct cgactgcatc
     5641 gaggcgg
//
LOCUS       DASULZ010000025         3010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_104518, whole genome shotgun sequence.
ACCESSION   DASULZ010000025 DASULZ010000000
VERSION     DASULZ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104518"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VFY96_00485"
     CDS             complement(<1..431)
                     /locus_tag="VFY96_00485"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX5604957.1"
                     /translation="MKPKLVIILGPTAVGKTQLALSLAQRINAEVVNADSQQVYRYLD
                     IGTGKASKAERERVRHHLIDVVNPDEDFNAALYRHLAAASIDEIHHRGAKVLVCGGTG
                     LYLKALTGGLFAGPGQDTELRAKLDREIAATGLASLYHRLIA"
     gene            complement(544..2277)
                     /gene="mutL"
                     /locus_tag="VFY96_00490"
     CDS             complement(544..2277)
                     /gene="mutL"
                     /locus_tag="VFY96_00490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEX5604958.1"
                     /translation="MKIQILSDDLASRIAAGEVVERPASVVKELIENSLDAGASEIFV
                     ALEKSGTALIRVADNGEGMDAEDLEMSVERHATSKLSVEADLFRIATLGFRGEALPSI
                     ASVSRMEIASRAKDAATAHLLSCEGGSRKELRPTAAAPGTTVFVREIFFNMPARRKFL
                     KSLATELSHICDVINRIALAHPAVHFRLAHDERTVADYTAVGQARDRVQQVLGREVVR
                     DIAPLSWRRSELLVQGFLSAAPASFPNSRYLYTYVNRRYVRDKVLTHAVLQGYENLLM
                     KGQYPAVVLFLDLPFEDVDVNVHPAKSEVRFRRQAEVHTAAASAVRNALKQAAKEPAV
                     RASFTSAETFQGVSEMALPYQMGSTAAQSFAHAVGDNFISALRTNEPDGYFSSLQILG
                     QILGCYIVCAAAHGLALIDQHAAHERVAFVKLRRQLETGQVSTQTLLLPQTLELNAGE
                     MSLVEANFETLKSFGFALEAFGPGTYAITEAPALLPEGDYTQLMRQMITDLADVDTST
                     KLRAHLEERLATIACHSVIRANRLLELDEIRALLTELDQIEFATQCPHGRPVLIEISR
                     EELERMFKRVV"
     gene            complement(2566..>3010)
                     /locus_tag="VFY96_00495"
     CDS             complement(2566..>3010)
                     /locus_tag="VFY96_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX5604959.1"
                     /translation="DVKGVWLTVSGGSRLMMVVSIKQRYPGHAKQAAIVASQCHAGAY
                     LGRFVVVVDDDIDPSNTDDVIWAMSTRCEPEQDIDILRRCWSGPLDPVIHPTKKGFNS
                     RAIIDATRPYEWMKDFPPVAESSKEVLDATAKKWGAELFGSNGKK"
BASE COUNT          651 a          893 c          806 g          660 t
ORIGIN      
        1 gcaatcagcc ggtgatataa ggacgccagc ccagtcgcgg cgatttcgcg gtctaatttc
       61 gcgcgcagtt ccgtgtcctg ccccggcccc gcaaataggc cgccggtcag cgccttcaag
      121 tacaacccgg tcccgccgca aaccaacacc tttgcaccgc gatggtgaat ctcgtcgata
      181 ctcgctgcag ccaggtggcg atatagcgct gcgttgaaat cttcatcggg attgaccaca
      241 tcgatcagat gatgtcgcac tcgctcgcgc tcggctttcg atgccttgcc ggttccgatg
      301 tcgagatagc gatagacttg ttgagagtcg gcgttgacaa cctcggcatt tattctttgt
      361 gccaggctta gcgccagctg ggttttgccg accgctgtcg ggccgaggat gatgacgagt
      421 ttcggtttca tcagaacgca gggctgcttt gttcgaaagt ccaacgttca aggttcaatg
      481 ttcaacgaac gatccggacg ctacactcct aacgttgaac cttgaacccg aacctcgaac
      541 agtctagacc acccgcttga acatccgctc gagttcctcc cgactgatct cgatcaacac
      601 cggtcggcca tgggggcact gggttgcgaa ctcgatttgg tcgagctcgg tgagcagcgc
      661 tcggatttcg tctaattcta acaaacgatt ggcacgaatg acgctatgac acgcgatggt
      721 cgccagtcgc tcttcgagat gggcccggag ttttgtcgag gtgtccacgt ccgcgagatc
      781 ggtgatcatc tggcgcatca gttgcgtgta gtctccctcc ggcagcagcg cgggcgcctc
      841 tgtaatcgca tacgttccgg gtccgaaagc ctcgagagcg aagccaaagc tcttgagagt
      901 ttcgaaattc gcctcgacca gactcatctc gccggcattg agctccagcg tctgcggcag
      961 caaaagcgtc tgcgtcgaaa cctgtccggt ttcgagctgc cggcgaagtt ttacgaaggc
     1021 gacccgttcg tgcgccgcat gctgatcgat cagggcaagc ccgtgcgcgg ctgcgcacac
     1081 aatatagcaa ccgagaattt gtccgagaat ttgcagcgag gaaaaataac catcaggttc
     1141 gttcgtcctt agcgcgctga tgaagttgtc cccaaccgca tgggcgaagc tttgcgcggc
     1201 ggtcgatccc atctggtagg gcagcgccat ctcgctgacg ccttgaaacg tttctgccga
     1261 ggtaaagctt gcgcgcacgg caggttcttt cgcagcttgt tttaacgcgt tccgcacggc
     1321 gctcgccgcg gctgtatgca cctccgcttg gcggcgaaaa cgtacctcgg atttggccgg
     1381 atggacgttg acatcaacgt cttcgaaggg cagatccaaa aacagcacaa cggcgggata
     1441 ctgccctttc atcaaaagat tttcgtatcc ttggagaacc gcatgggtga gcactttatc
     1501 gcgcacgtag cgccggttca catacgtgta gagataccgt gagttcggaa aagaggccgg
     1561 tgccgcactc aaaaagcctt gcaccaatag ttcgctccgt cgccagctca acggtgcaat
     1621 atccctaacc acctcccgcc ccaacacctg ctgcacacga tcgcgcgcct gcccaacggc
     1681 ggtataatcc gcaaccgtgc gttcgtcgtg cgccaggcga aaatgaaccg ctggatgagc
     1741 gagcgcgatc cgatttatga catcgcagat atggctcaat tccgtcgcca gcgatttgag
     1801 gaacttgcga cgcgccggca tgttaaaaaa gatctcgcgg acgaaaaccg tcgtaccggg
     1861 agcagccgcg gtcgggcgaa gctctttcct actcccgccc tcacaagaga gtaaatgcgc
     1921 agtggctgcg tccttggcgc gcgaagcgat ttccattcgt gataccgacg cgatactcgg
     1981 cagggcttcg ccgcgaaacc ccaacgtggc gatccgaaac aaatccgcct cgacgctcaa
     2041 cttgcttgtg gcatggcgct cgacggacat ttccaggtct tcggcgtcca taccttcccc
     2101 attgtcggcc acgcggatca gtgccgtgcc gcttttttcc agggcgacga aaatctcgct
     2161 ggcacccgca tcaagcgaat tctcgatcag ttccttcacc accgaggccg ggcgctcaac
     2221 gacttcgccg gccgcaatgc ggctggcaag gtcatcggac aaaatctgga ttttcaccgt
     2281 aattctctaa tgtgatatcg ctaaggtccg ttatattttc aggacactgg aagccgacaa
     2341 tactgactgc ctgtggcgcg gtcaaggtaa gtttctcgct agcaagcaaa gggggcctgt
     2401 ctgtgcgcct caggagcaat ccatctgtgt agccgcatca aaaacagttt ccaattcggc
     2461 tccagatttg ctggcgacgt aattcaaccc ctgcaagata aaataaaaaa ggcaagtcga
     2521 tctaacgatc gacttgcctt ttttgtcagt agtcagtcgc tacttttact tcttaccgtt
     2581 gctgccgaat aactcagcgc cccatttttt cgcggtggca tcgagcactt ctttgctcga
     2641 ctcagcgacc ggcggaaagt ccttcatcca ttcgtacggc cgagtcgcat cgatgatcgc
     2701 gcgcgagttg aaaccctttt tcgtcggatg aatcaccgga tccagcggtc cactccagca
     2761 gcggcgcaga atgtcgatat cctgttcggg ctcgcagcgg gtcgacatgg cccagatcac
     2821 gtcatcagta ttcgatggat cgatatcatc atcgacgaca acgacaaagc ggccaagata
     2881 agcgccggcg tgacattgcg aagcaacgat cgcggcttgc ttggcatgac ccggatagcg
     2941 ctgtttgatc gagaccacca tcatcaatcg gctgccgcca gaaaccgtaa gccacacacc
     3001 tttgacgtcg
//
LOCUS       DASULZ010000026         5001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1220721, whole genome shotgun sequence.
ACCESSION   DASULZ010000026 DASULZ010000000
VERSION     DASULZ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5001
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220721"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VFY96_00500"
     CDS             <1..843
                     /locus_tag="VFY96_00500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HEX5604960.1"
                     /translation="SRKSHDELLKFIGKKPYNYRLALLDGDASLAGLWVNKDDYTHER
                     TLGGIAAIDPSLISIEAVEAYLLEKTKDQRRSNAARAAYESLRQEAKPPRVRTVTPDE
                     GIEWPHAVPVLPKWFEMQEGAVVPAVQRGFEMGPRGQNRNPGFKRGTTKTQRPVVRFD
                     LCTKCTLCWAECPDECFDPTTDGFYDVDYQYCVGCGKCAQVCPVKECIVMVDELQFED
                     DSSPWEHWKRTPQEYIQWAETKKGKERVSYPVVTGKGMTVTQGELMPEGKIVPVRRTE
                     EVET"
     gene            840..2084
                     /locus_tag="VFY96_00505"
     CDS             840..2084
                     /locus_tag="VFY96_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013604565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HEX5604961.1"
                     /translation="MSVAVAQTKSDESGGVYEREDLLNGCQAVAQGVCLADVDVIAAY
                     PIRPYTEVMDALSKLIADGKFDAEYIIADSEHSQFEIVKHASSVNARAFGGSSGTGWM
                     YGMEALVVTATDRLPPLFVVGNRALDDPGAFGVEHNDAMAVRDMGWLLCWVTTPQEAL
                     EHVLIGYRIAEDKKVRMPMGLAMDGAFLTHSQHMVKIPSVEAAKRFLPQYDLAERRLH
                     PDNPISVAPQANEDWVMEIRRQNWEAAKRARGVIKDAYKEFNSVFGDRYAAPYYFDEF
                     MTDDAEAVLIGIGTVAAPARTAVRRLREKGQKVGYVNLRWFRPFPTVELRECLSRFKA
                     VGVVDRDFAHGSPDNCGILMHEVRSCLYPAKNRPAIVNFIGGLGGRDLSIADTQKMFE
                     TTQQAAKKDSMDEFITWIGLRE"
     gene            2100..3095
                     /locus_tag="VFY96_00510"
     CDS             2100..3095
                     /locus_tag="VFY96_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012021147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5604962.1"
                     /translation="MSTLPTIDYERIKGVKNVPLEELYTSGHRTCQGCESATTMRGFI
                     KSAGSRTVVTGATGCMYVANTSYMTTPWIVPWMHTQLGAGGSSAVGMAAGLRALKRKG
                     KIKDEKINVINFSGDLGAGDMGIGGISAALQSDYDLLIIMYDNESAANTDIQATGLST
                     YGAQTTFTPPGSKHPIMQRRWKKNVAGMLAVGHPTCKYVATACATYPPTDYLNKVRKA
                     LEIGGPTFIHTYDPCPKGWDYHPRYSNELGELGIKCGLYPLYEIVEGNVIYTYDARKS
                     GKGRIPVTELLTKQGRFDHLKEEHFTYIQKMVDQMWEEWQIPGVAPLRGILKARI"
     gene            3473..>5001
                     /locus_tag="VFY96_00515"
     CDS             3473..>5001
                     /locus_tag="VFY96_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5604963.1"
                     /translation="MAIANKSDGSKEATGPVISGGHLVAKALKNEGVEVIWTLCGGHI
                     IDIYDGCLDEGIKIIDVRHEQVAAHAADGYSRQSGKIGCVVTTAGPGTTNAMTGIANA
                     FRAESPLLMIGGQGALSQHKMGSLQDLPHVDIMSPITKFAASVPHTERIADMVSMAFR
                     EAVNGAPGPSYLEIPRDVLDAKVPIERARIPKPGAYRASTKSTGDPADIEKLADLVVN
                     SERPAALFGTQVWTCRGHEAAVEFCRALNVPGYMNGSARGIFAPGDPHGFNRTRSYAF
                     DKADVILIVGCPFDFRLGYGRRLRADATVVQIDLDYRNVGKNRDISLGLVGDCGAILK
                     AVAQAATGRASKKNTQRAEWMKELRAAEAKAYEKLLPSFKSESVPINPYRVAYEINEF
                     LTDNTVYIGDGGDVVTITATAVEPRKAGNWMDPGPLGTLGVGTSFSMATKFVHPKKEV
                     LCYYGDGSFGMTSWDMETAQRFKLPYMAVIGNNSAMNQIRCGQIGKYGPERGGVGNKL
                     G"
BASE COUNT         1131 a         1395 c         1507 g          968 t
ORIGIN      
        1 tccagaaaaa gccatgatga actgctcaag ttcatcggca aaaaacccta taactatcgc
       61 ctggcgctgc tcgacggtga cgcgagcctc gccggactct gggtaaacaa ggacgattac
      121 acccacgagc gaactctcgg cgggatcgcg gccatcgacc cgagcctgat cagcatcgag
      181 gccgtggaag cctatctgct cgaaaaaacc aaagaccagc gccggtcgaa tgctgcgcgc
      241 gccgcgtacg aatcgctgcg ccaggaagca aagccgccgc gcgttcgaac cgtgacgccg
      301 gacgaaggca tcgaatggcc gcacgccgtg ccggtgttgc caaagtggtt cgagatgcaa
      361 gaaggcgccg tggttccggc ggtgcagcgc ggcttcgaga tgggtccgcg gggacaaaat
      421 cgaaaccccg gctttaagcg cggcacgacc aagacgcagc gcccggttgt gcgattcgat
      481 ctttgcacga aatgcactct ctgctgggcc gaatgcccgg acgagtgctt cgacccgacg
      541 acggacggtt tttacgacgt cgactatcag tattgcgtcg gctgcggcaa gtgcgcgcag
      601 gtgtgtcccg tcaaagaatg catcgtgatg gtcgatgagc tgcaattcga ggacgactct
      661 agcccgtggg aacactggaa gcggacgcct caagaataca ttcaatgggc agaaaccaag
      721 aagggcaaag agcgggtttc ctatccggtg gtcaccggga agggaatgac ggtgacccag
      781 ggagaactta tgccggaagg aaaaatcgtg ccggttcgaa ggactgagga ggtggaaaca
      841 tgagcgttgc agttgcacaa actaaatcag atgaatcagg cggtgtctac gagcgggaag
      901 atctcttaaa cggctgccag gcggtcgcac aaggggtgtg tcttgcggac gtggatgtga
      961 tcgccgctta tccgatacgt ccctacaccg aggtaatgga cgctctctcg aagctgatcg
     1021 ccgacggaaa attcgacgcc gagtatatta tcgccgacag cgagcattct cagtttgaga
     1081 tcgtcaagca cgccagttcc gtaaacgccc gcgccttcgg cggatcgagc ggcacgggtt
     1141 ggatgtacgg tatggaagcg ctggtcgtta ccgcgaccga ccgcctgccg cctcttttcg
     1201 tcgtcggcaa ccgcgctctg gatgatccgg gcgccttcgg cgtcgagcac aacgacgcga
     1261 tggcggtccg cgacatgggc tggctgctct gctgggttac gacgccacag gaagcgctcg
     1321 agcatgtgct gatcggctac cgcatcgccg aggacaagaa agtcagaatg ccgatgggct
     1381 tggcgatgga cggcgccttt ctcactcatt cgcagcacat ggtgaagatt ccatccgtag
     1441 aagccgcgaa gagatttctc ccacagtatg accttgccga gcgccggctc catcccgaca
     1501 acccgatctc ggtcgcgcca caggccaatg aggactgggt catggaaatt cgccggcaaa
     1561 attgggaagc cgccaagcgc gcccgcggcg tgatcaaaga cgcgtacaaa gaattcaaca
     1621 gcgttttcgg cgatcgttac gccgcgcctt actatttcga tgagttcatg accgacgatg
     1681 cggaagcggt gctgatcggc atcggcacag tggcggctcc agcgcgcacg gcggtccgac
     1741 gtttacgcga gaagggacag aaagtcggct acgtgaacct gcgctggttc cgtcctttcc
     1801 cgacggtaga gttgcgcgag tgtttgagcc gcttcaaagc cgtcggcgtc gtggaccggg
     1861 acttcgcgca cggatcgccc gacaattgcg gcatcttgat gcacgaggtc cgttcctgcc
     1921 tctacccagc gaagaaccgg ccggcgatcg tcaacttcat tggcggcttg ggcggtcgcg
     1981 atctgtcgat cgcagacacg cagaaaatgt ttgaaaccac tcaacaggcg gccaagaagg
     2041 attccatgga tgaattcata acctggatcg gtctacgaga ataaaggaga ggaaccatta
     2101 tgtcgactct accgacaatc gattacgaaa gaattaaagg cgtcaaaaat gttccgctcg
     2161 aagagctcta cacatcgggt caccggactt gccagggctg cgagtcagct accaccatgc
     2221 gcggattcat caaatcagcg ggatcgcgca ctgtggtcac gggcgcgacc ggctgcatgt
     2281 atgtggcaaa tacgtcgtat atgacgacgc cgtggatcgt gccgtggatg cacacgcagc
     2341 tcggcgccgg cggttcttca gcagttggca tggccgcggg tctccgcgcg ctcaagcgca
     2401 agggcaagat caaagacgaa aagatcaacg tcatcaattt ttccggcgat ctgggcgccg
     2461 gcgacatggg catcggcggc atctccgccg cgctgcagag cgactacgat ttgctgatta
     2521 tcatgtacga taacgagtcg gcggcgaaca cagatattca agctaccggg ctttcaactt
     2581 acggcgcaca gactacgttt acgcctcccg gcagcaagca tccgatcatg cagcggcgct
     2641 ggaaaaagaa cgtcgcgggt atgctcgccg taggacatcc gacgtgcaag tacgtcgcca
     2701 ccgcatgcgc gacctacccg ccgacggatt atttgaacaa ggtacgcaaa gcgcttgaga
     2761 tcggcggtcc gacttttatt cacacctacg atccctgccc gaagggttgg gattatcacc
     2821 cgcgctactc gaatgagctc ggcgagctgg gcattaaatg cggtctctac ccgctttatg
     2881 aaatcgtcga aggcaatgtc atctacacct acgatgcacg caaatccggc aaggggcgga
     2941 ttcccgtaac agaactgctc accaagcagg ggcgcttcga tcatctcaaa gaagaacact
     3001 tcacctatat ccagaagatg gtcgatcaaa tgtgggaaga gtggcaaatt cccggtgtcg
     3061 cgccgctgag aggaatactc aaagccagga tctaattcgc cttgtcattg tccaaactga
     3121 ctgggccgct ggttgcggcc ccagtcttta ttgaggttcc atcgtcggag cgtcacatag
     3181 gaaagattcc tccgctccgt tcgggatgcc gtccgcgcat ttctccagtg cttgagcgtc
     3241 ggagcggtat tctgtatcct aacctgatac gagacgcggc ggttaggaca ctgattatag
     3301 ctcctgccgg ttagagcgat ccgctctcat atacaaatcc gagagcaatt tatttgcgcc
     3361 acgttgaata gtggcgggct ctaaatgcca agccgctttg ggcaaaacag acgaacgagg
     3421 cggcggcatt ggttagtatt gttgtagctt tatagtggga tgagaggtct ttatggcgat
     3481 tgcaaataag tccgacggca gcaaagaagc gaccggaccg gtgatctcgg gtggtcactt
     3541 agtcgcgaag gcgctcaaga acgaaggtgt ggaagtgatc tggacgctct gcggcggcca
     3601 tatcatcgat atctacgacg gttgtctcga tgaggggatc aagatcattg acgtccgtca
     3661 cgagcaggtt gccgcgcatg cggcggacgg ctattcgcgc cagagcggca aaatcggctg
     3721 tgtcgtgacc acggcgggtc cgggcacgac gaacgcgatg acgggcattg ccaatgcatt
     3781 ccgcgctgaa agtccattgc ttatgatcgg cggccagggc gcgctcagcc agcacaaaat
     3841 gggctcgctg caggacttgc cgcatgtcga tattatgtcg ccgatcacga aattcgccgc
     3901 cagcgtgccg cataccgagc gcatcgcaga catggtttct atggcatttc gcgaagcggt
     3961 caacggcgcg ccgggaccga gctatttgga gattccgcgc gacgtgctcg atgccaaggt
     4021 gccgatcgaa cgcgcgcgta tccccaagcc aggcgcatat cgcgcttcga cgaaatccac
     4081 cggcgatccg gcggacatag aaaaattggc cgatctcgtg gtcaattctg aaaggccggc
     4141 ggcgctcttc ggtactcagg tctggacgtg ccgcgggcac gaggcggcag tggaattttg
     4201 ccgcgcgctc aacgtacccg ggtacatgaa tggctcggca cgcggcatct tcgcgccggg
     4261 tgatccgcac ggattcaatc gcacgcgcag ttacgccttt gacaaagcgg atgtgatttt
     4321 gatcgtcggc tgcccgttcg attttcgttt gggttatggc cggcgtttgc gcgccgatgc
     4381 gaccgtggtg cagatcgatc tcgattatcg caatgtcgga aaaaatcgcg atatctcgct
     4441 cggcctggtc ggcgactgtg gcgcgattct aaaagccgtc gcgcaagcag ccaccgggcg
     4501 cgcatcgaag aagaacacgc aacgcgccga atggatgaag gagttacggg ccgctgaagc
     4561 caaagcctac gaaaaactgt tgccgtcgtt taaatcggaa tcggtcccga tcaatcccta
     4621 ccgggtcgca tatgaaataa acgagtttct caccgacaac acggtgtata tcggcgacgg
     4681 cggcgacgtg gtgacaatca cggccacggc cgtagaaccg cgcaaggccg gcaactggat
     4741 ggatccgggg ccgctcggca cgcttggcgt cggtacttcc ttctcgatgg ccacgaagtt
     4801 cgtccaccct aaaaaagaag tgctctgcta ttacggcgat ggctcgttcg gtatgacgtc
     4861 ctgggacatg gaaaccgcgc agcgcttcaa gctgccgtac atggcggtga tcggcaacaa
     4921 ttccgcgatg aatcagattc gctgcggaca gatcggcaaa tacggtccgg agcgcggcgg
     4981 cgtcggcaac aagctcggcg a
//
LOCUS       DASULZ010000027         5690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1322379, whole genome shotgun sequence.
ACCESSION   DASULZ010000027 DASULZ010000000
VERSION     DASULZ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5690
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322379"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VFY96_00520"
     CDS             <1..503
                     /locus_tag="VFY96_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5604964.1"
                     /translation="IIRQEESTKSDGRVDTWSYFKDSKMVRREVDTKQLGRPDTVYFY
                     DNDKIVREERDEAGLGSITYRAIYQNGRLAKLEKDTNGGGRPDLWIYYDASQDGEIVL
                     KEERDLNGDGVADLWTYYENGRLVRRDVSAVGFEILSKKEELPAAVTELTKISVPGNE
                     NAADQR"
     gene            484..1506
                     /locus_tag="VFY96_00525"
     CDS             484..1506
                     /locus_tag="VFY96_00525"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX5604965.1"
                     /translation="MQQINADWEDFSVLSLFRSPFLIPSVLFHSMLALLALRAAMLPI
                     AKPEETPISVQLLEPREGTSPNKSIGRASGPGGPRTMPKLGTPTPPMQRTGKLDQGSV
                     ETSIPNNTPTETAPPPKPAPLPAPKVLASDSRAESVNVKETSPDSLVHLPTKESPTNL
                     PGNAADLDLRQQQSPAALKGTGEGPGIKALKDGAQIPGALKGTGTGLGPYGVPGGTST
                     GTGVTGGGTGIGNGGGSTTGLRGLSSADYNQYLSQLKKRVESVWKYPDGVNGVQKVAI
                     LFTLDKAGRLMRSEVLESTDARLNASAVEAMKRASPFPPIPETLKDLANTPLRMQFTV
                     SIGVRG"
     gene            1599..2225
                     /locus_tag="VFY96_00530"
     CDS             1599..2225
                     /locus_tag="VFY96_00530"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX5604966.1"
                     /translation="MQPQGLDLIDIIHKGAIATYPLILLSIISLTVVFERLWSLRNLG
                     AITLRVTESILEPVKKGQRDLAIAICKQNSNWPVARIFLNILERESSARMEAANSIVT
                     EALFEETQKLKKNLWILGTVASSAPFIGLLGTVVGIIKSFESMAIAGTGGFAVVAAGI
                     SEALVATALGLGVAIIAVIFYNYFQTRIAMLTGLFRIQAAKVVQLMSA"
     gene            2233..2658
                     /locus_tag="VFY96_00535"
     CDS             2233..2658
                     /locus_tag="VFY96_00535"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX5604967.1"
                     /translation="MAMTPIGGNEDSSEIVAEINITPLTDIFLVLLIIFMITSSAMIE
                     SGGKVNLPKAVATQSESRGTTVTITPKHEIFVDQKIVKEENLEKILQDALNARANKTV
                     ILRGDKDVLLGDTVKVMSMIKRAGASEIAIAAETERKSR"
     gene            complement(2683..3876)
                     /locus_tag="VFY96_00540"
     CDS             complement(2683..3876)
                     /locus_tag="VFY96_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5604968.1"
                     /translation="MNSLASAQPKVQILSPKNGTQITQDQKSVLVSGKVARETGRSAN
                     VDVFLLIDVSGSTALYAGADLGDADQPPQSTGFPVPQISIGGFGLGGPLMRNPRTSVL
                     AAEVAAARRLLLQLNHETTRVGIVSFSDRANLLQPLTHDFERVRKVLADVLRAGSYGG
                     TNMVEAIRLGISELMGFGSSERRTDAIKVQFLLTDGFPTLPIGESKRMTPEDVALTIN
                     AARLAGKAGIKIHVFALGEEALSYPRAASGIAKESGGTYTPVMRPADALAVLENISVV
                     GVDYVQVINQTIGQNATQLRLAADGFFSSAVPVTEGKNQIDVLARASDGANSRESITV
                     YYQPGTQKSLELEVFLEKEKNLQLEVNRLGKSPAEIQREVERNREDSLSKPQQLPPPT
                     DGPAR"
     gene            4040..4771
                     /locus_tag="VFY96_00545"
     CDS             4040..4771
                     /locus_tag="VFY96_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5604969.1"
                     /translation="MLKATLIAALLCVSFGIADAQYVVVLKNGRQLTVQNYREEGSVI
                     KFFGFGGEIAISKDQVQTIRRADTSESGAAPRASLDRPPAETAAESPPAPSAQPEIRA
                     PAAVSLDQQQAKKRAEEAKAYESRLKELTAQLKELRERYSLMTRGNQGPEPSFFTTEE
                     AFRGQQEDLLSRLRDAQNKAQGLPLGGAATSPPFSLDPPPAYSDRQKQLSDLRARITQ
                     IENERQKIIDEMRAKGFEVGSLFLE"
     gene            complement(4757..5191)
                     /gene="aroQ"
                     /locus_tag="VFY96_00550"
     CDS             complement(4757..5191)
                     /gene="aroQ"
                     /locus_tag="VFY96_00550"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015199325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX5604970.1"
                     /translation="MQILVLNGPNLNLLGKRQPEIYGSLSLSDIEQQVRALAKELGVE
                     VDFRQSNSEAELINWIHEAAGKFGALVINPAAYTHTSLAIRDAISAVGIPTVEIHISN
                     IHRREPFRHHSYIAEVAVGQIAGFGAISYLLGIRAAVELLQK"
     gene            complement(5234..>5690)
                     /locus_tag="VFY96_00555"
     CDS             complement(5234..>5690)
                     /locus_tag="VFY96_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX5604971.1"
                     /translation="IVALDRELLERIVVTSCSIKARIVEMDEREENQRAVLNFGHTIG
                     HALEAATDYREFLHGEAVGIGMVKATMLSVQHGFCDQQTFERILKVIKQAGLPSEIPA
                     GLSRQSVIEAMQLDKKAAGGRIKFVMSEGIGKTRFHWLLPGEVLAALET"
BASE COUNT         1347 a         1591 c         1541 g         1211 t
ORIGIN      
        1 aaattattcg ccaagaagaa tcgacaaaga gcgatggccg cgttgatacc tggagctatt
       61 tcaaagacag taagatggtt cgtcgtgagg tcgataccaa acaactcggc agacccgata
      121 cagtgtattt ttacgataac gacaaaattg ttcgcgaaga gcgtgacgag gccggcttgg
      181 gaagtatcac ctatcgcgcc atctatcaga atggccgact cgccaagctc gagaaagaca
      241 cgaacggcgg cggccggccc gatctgtgga tttattatga tgccagtcaa gatggcgaga
      301 tcgtcctcaa agaagagcgc gatctcaatg gcgatggcgt cgcagattta tggacgtatt
      361 atgaaaatgg tcgcctcgtt cggcgcgatg tcagtgccgt cggtttcgaa atcctctcaa
      421 agaaagaaga gcttcccgca gcggtgaccg aattgacgaa aatttctgtt cccggaaatg
      481 aaaatgcagc agatcaacgc tgattgggaa gactttagcg ttctctcgct gtttcgcagt
      541 ccttttctca tcccctcggt gttgtttcac tcgatgcttg cgttgctcgc attgcgtgca
      601 gcgatgttgc cgattgcgaa acctgaagag acgccgattt ccgttcagct actggaacca
      661 agagaaggga catcgccgaa caagagtatc ggtcgagcca gcggtccggg cggcccgcgc
      721 acaatgccga aattggggac accgacacca cccatgcaac gaaccggcaa attggatcaa
      781 ggctccgtcg aaacctcgat tccaaacaat actcctactg aaaccgcacc gcctcccaag
      841 ccagcgcctt tgcctgcccc aaaagtcttg gcttcagact cgcgcgctga atcagttaac
      901 gtaaaagaaa cgtcaccgga ttcattggtg cacttgccta ccaaggaaag tccgacaaat
      961 cttcccggca atgctgctga tctcgacctc cgtcagcagc aatccccggc agcgctcaaa
     1021 ggcacgggag aaggccccgg gatcaaggcg ctcaaggacg gcgcgcagat tcccggcgcc
     1081 ctcaagggaa cagggacggg cttaggcccg tacggcgtcc cgggcggcac tagtacggga
     1141 acaggcgtaa cgggcggggg tacaggaatt ggcaacggcg gcggcagcac gaccggactg
     1201 agggggttgt cgagcgccga ttacaatcaa tatctgagcc agctcaagaa acgagtggag
     1261 tccgtgtgga agtatcctga cggtgtcaac ggcgtgcaaa aagtcgcgat cttattcacg
     1321 ctcgacaaag cgggcagact gatgcgctcg gaggtgctgg agtccaccga cgcgagactg
     1381 aatgccagcg cagtcgaggc gatgaagcgc gcctccccat tcccgcccat tcccgagaca
     1441 ttgaaagacc tggccaacac ccctctgcgc atgcagttta ccgtcagcat cggcgtgcgc
     1501 ggttagctat cccctcagag acactccgga ttccccgggg ttcgttgcaa taattttcct
     1561 ataaggtaat catcatccga tcatcggaag gaaatttcat gcagccgcaa ggtctcgatc
     1621 tcatcgatat tattcacaaa ggcgccatag cgacttaccc gttgattctc ctgtcgatca
     1681 tcagcttgac cgtggtgttt gaacgtctct ggtcgctcag aaatcttggt gcgattacac
     1741 ttcgagtcac tgagtccatt ctcgaaccgg tcaagaaagg gcagcgcgat ttggcgattg
     1801 ctatatgtaa gcaaaactcc aattggcccg ttgcgagaat ttttttgaac atcctggaac
     1861 gcgaaagcag cgcgcgcatg gaagccgcca acagcatcgt caccgaagcg cttttcgaag
     1921 aaactcagaa attgaagaaa aatctctgga tcctcggcac cgtggccagc agcgcgccgt
     1981 tcatcggtct gctcggcacc gttgtcggaa tcatcaaatc cttcgaaagt atggcgatcg
     2041 ccggaacggg cgggtttgcg gtcgtggctg cagggatatc cgaagccttg gtggctacgg
     2101 ctttgggtct tggggtcgcg attatcgcgg ttatttttta caactatttc caaacccgga
     2161 tcgcgatgct cacgggcctc tttcgcattc aagccgccaa agttgtccag ctcatgagcg
     2221 cgtgaggatt gtatggcgat gacaccgata ggcggaaatg aagacagttc cgagatcgtc
     2281 gccgagatca atatcactcc attgactgat attttcctcg tgctgctgat cattttcatg
     2341 atcaccagtt cggcgatgat cgagtccggc gggaaagtga acctgccgaa agcggttgcg
     2401 actcaatcag aatcacgcgg cacaaccgtg acgatcacgc cgaaacacga gattttcgtc
     2461 gaccaaaaaa tagtcaagga agagaatctg gaaaaaatcc ttcaggatgc gctcaacgct
     2521 cgcgccaaca agaccgtgat ccttcgtggc gacaaggacg tgctgctagg cgatactgtt
     2581 aaggtgatgt cgatgatcaa acgcgccggc gcttctgaga tcgccattgc ggctgaaacg
     2641 gaaagaaaaa gtcgctaggc caggcgcaga gctttctcaa gctcatcgcg ccggtccgtc
     2701 ggtgggcggc ggcagctgct gcggcttgct aaggctatct tcacggttgc gctcgacttc
     2761 ccgctgaatc tccgccggac ttttaccgag ccggttgact tcaagttgca gatttttttc
     2821 cttttctagg aagacttcca actccaagga tttttgtgtg ccgggctgat agtaaaccgt
     2881 gatcgattca cgactgttgg caccatcact cgcccgcgcc aaaacatcga tttgattttt
     2941 tccttccgtg accggtaccg cggaggaaaa aaacccatcg gccgccaacc gtaattgcgt
     3001 cgcgttctgc ccaatcgtct ggttgatgac ctgcacgtaa tcgacaccga ccaccgaaat
     3061 gttttccagc accgcaagcg catccgccgg tcgcatcacg ggcgtatagg tgccgccact
     3121 ctctttggca ataccactcg cggcgcgcgg gtaagagagc gcctcctccc caagagcaaa
     3181 gacgtggatt ttgatgccgg cttttccggc caaccgtgcc gcattgatcg tcagtgcgac
     3241 gtcctccggt gtcattctct tactttcacc gatcggcagg gttggaaatc cgtcggtgag
     3301 aaggaattgc actttaatgg cgtcggtccg cctttcgctg ctgccgaatc ccattagctc
     3361 gctgatgcct agccgtatcg cttccaccat attggtaccg ccgtaagaac ccgcccgcag
     3421 cacatcggcc aagacttttc gaactctttc gaaatcatgg gtgagcggtt gaagcaaatt
     3481 ggcgcggtcg ctgaagctca cgatgccgac ccgcgtcgtt tcatgattga gctgaagaag
     3541 caggcggcgt gccgccgcca cctcggcggc cagaacggaa gttcgcgggt tgcgcatgag
     3601 cggtccgccg agaccgaaac cgccaatgct gatttggggc accggaaaac cggtgctttg
     3661 gggaggctga tcggcatccc cgagatccgc gcccgcataa agtgcagtgc tccctgaaac
     3721 atcgataagt aaaaagacat cgacattcgc cgatcgcccc gtctcgcgcg cgaccttacc
     3781 gctgaccaaa actgattttt gatcctgggt gatctgggtg ccgtttttgg gcgaaagaat
     3841 ctgtactttg ggttgtgcgg aggcaaggga gttcaaacca acaatgcaca agaagcatga
     3901 agcgctaacg agagcacatc gaaacaggtt catagatcaa cctctccgac agtgccgacg
     3961 ctagcatgat tgctcggggc ataaaagagc cgttccagac gcatacctga atagaggtca
     4021 tcgtgttata atccgtccaa tgttgaaagc taccctgata gcagcgctgc tctgcgtgag
     4081 tttcggcatc gcggacgcac agtacgttgt cgttttaaag aatgggcggc aactgacggt
     4141 gcagaactat cgcgaggaag gcagcgtcat taaatttttt ggtttcgggg gagagatcgc
     4201 catcagcaag gatcaggttc aaacgatccg ccgcgcagac acctcggaga gcggcgctgc
     4261 gcctcgcgcg tcattagatc gtccgccagc ggaaactgcc gccgaaagtc cgccggcgcc
     4321 gagcgcacaa ccggagatta gagcaccggc tgcggtttcc ctcgaccaac aacaagctaa
     4381 aaagcgcgct gaagaagcca aagcgtacga gagcaggcta aaagaattga ctgcacaact
     4441 gaaggaattg cgggagcgct attctttgat gacacgaggt aaccaggggc ccgaaccgag
     4501 tttttttacc acggaggaag ccttcagggg ccagcaggaa gacctattgt cgcggcttcg
     4561 tgacgcccag aacaaagctc aagggctgcc gctcggcggc gctgcgacgt cgccgccgtt
     4621 ttcactcgac ccgccgccgg cctattccga ccggcaaaag cagttgagcg atttgagagc
     4681 tcgtatcact caaatcgaaa acgagcggca aaaaataatc gacgaaatga gagcaaaagg
     4741 attcgaagtc ggttcgctat ttttggagta actccaccgc ggcgcgtatc cccaataggt
     4801 aactgatcgc tccgaagccg gcgatctgac cgacggccac ttccgctata taggaatgat
     4861 gtcggaacgg ctcgcgcctg tgtatattgg aaatgtgaat ctccactgtc ggaatcccca
     4921 ccgcggaaat agcgtctcgt attgccaggc tggtatgggt gtacgccgcg ggattgatga
     4981 ccagagcgcc gaacttcccg gcggcctcgt ggatccagtt gatcaattcg gcctcgctgt
     5041 ttgattgacg aaaatcaact tcgacgccaa gctccttggc aagcgcgcgc acttgctgct
     5101 cgatatctga aagactcagg cttccatata tctccggctg tcgtttgccg agcagattga
     5161 gattggggcc attcaatacc agaatctgca cgtccgccat tgagtcacct ccgtcggaac
     5221 cacgctctcc aagctacgtc tctaatgcgg ccagaacttc tccaggcaaa agccagtgaa
     5281 aacgtgtttt cccgatccct tcagacatga caaacttgat ccggccgccg gctgcttttt
     5341 tatccagctg catagcttcg atcacactct gccgcgagag cccggcggga atttcgctcg
     5401 gcaacccggc ttgtttgatg accttgagaa tgcgctcaaa ggtttgctga tcacaaaaac
     5461 cgtgctgcac cgagagcatg gtggctttga ccatgccgat tcccacggct tcgccgtgca
     5521 gaaattcccg gtaatccgtc gcggcttcca acgcatgccc aatcgtgtgc ccaaagttga
     5581 gcacggcgcg ttgattctcc tcgcgctcgt ccatttccac gattcgcgcc ttgatggaac
     5641 aagacgtcac gacgattcgc tccaataact cgcgatcgag cgcgacgatc
//
LOCUS       DASULZ010000028         3577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_206153, whole genome shotgun sequence.
ACCESSION   DASULZ010000028 DASULZ010000000
VERSION     DASULZ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206153"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            325..651
                     /locus_tag="VFY96_00560"
     CDS             325..651
                     /locus_tag="VFY96_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5604972.1"
                     /translation="MLRNMQSEIENQVRPLAQQAIDLEVQRLREQAAIDERDLRACLE
                     RIDRCVSACIQRAHEYRREHAELAGFNRRLAALGSSAEPMPECPSVITEIIKSRLKWL
                     RVQQKA"
     gene            complement(1236..1457)
                     /locus_tag="VFY96_00565"
     CDS             complement(1236..1457)
                     /locus_tag="VFY96_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5604973.1"
                     /translation="MFPTRTGWWVITQLQPITVIFTVPEDNLPQILRYIKTGQKLPVE
                     AYDRKQKRSLATGALASIDRIDPQPKPSS"
     gene            complement(1571..1927)
                     /locus_tag="VFY96_00570"
     CDS             complement(1571..1927)
                     /locus_tag="VFY96_00570"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEX5604974.1"
                     /translation="MPEDLAREVREQATHQICSNSAAPYNTITVKSRVDGQLVNVLFK
                     EGQTVSGGELLAEIDPRPFQVHLAQPKVKERGIRLVDQRQKSIWNGTACCFPKTPVPK
                     QQLDTQESLVRQYEVW"
     gene            complement(2069..2449)
                     /locus_tag="VFY96_00575"
     CDS             complement(2069..2449)
                     /locus_tag="VFY96_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5604975.1"
                     /translation="MPSQENLTERARRRLSISKRKLLGWEKYYLFQTRGTAPKRAVLI
                     NNLVRDTLMLLQPLFDERTIEVTSNCSGAAPVIYGNSGSIQRVLIKVLDNAMYACEKA
                     GAIGARSKFLPGVLARAQTGRCSY"
     gene            complement(2463..2600)
                     /locus_tag="VFY96_00580"
     CDS             complement(2463..2600)
                     /locus_tag="VFY96_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5604976.1"
                     /translation="MRAEIIKRKKAETELLRVQLELGRVERLATLGRMAEPLRTMWAR
                     R"
BASE COUNT          811 a          967 c          992 g          807 t
ORIGIN      
        1 cagtcgcagt gacgttctgg aattggtctc cagcaaatcg ctgaatttag gggaggcgag
       61 cgagagtcct ctgcgttcag ccgccggcgg cgcaaaatag acgtcttgag attcggtcca
      121 agcagtggaa gaccaagatc caatcaagga actacaatcc cttctgaccg accgaagcaa
      181 agtgctggaa cgtatctccg gcatccatgc catgttggat tccatgaagg ccccggtatc
      241 gagtgcagct agtactgaaa ccactcgaaa ccggtatcgc ttgctccagc gtcaagtgaa
      301 aacccctatg acaggctttt gcatatgttg cgcaatatgc aatcggaaat agaaaatcag
      361 gtgcggccat tagcgcagca agcgattgat cttgaagtac aacgtttgcg cgagcaagcg
      421 gcaatcgatg aacgagactt acgtgcctgc ctggagcgga tcgatcggtg tgtatccgca
      481 tgcattcaac gcgcccatga gtaccgacgc gaacacgcgg aacttgcggg tttcaatcga
      541 cgattggctg cgcttgggtc gtcggcggag ccgatgcccg aatgtccctc ggttatcaca
      601 gagattatca agtcccggtt aaagtggctg cgtgtgcagc agaaggcgta gcctcagaac
      661 tcacgatatc taagacgttt tccaacctcc gccaagcgct ttcaccaata aaacggcagt
      721 gttcagccgc cggccaagaa gatccatcgc gacgcgttga ttgctgaacg cagttgttag
      781 tgccacgacc acgttcaaat acctgacgat tcccgcgcga tactggttcg tgattacagc
      841 tagagattgt tgcgcagctg tgactgcctc atcttgcgct tgcgcctcgc tggtgagaat
      901 tcgctgcgcc atatattacc ccgactcctt gagtgtcgcg cgagcttggc gcggagtcgc
      961 ccgatcacct gatcggggcg aagatcgcgc tcagccaagg gcttacgcgg cataaagatg
     1021 ttgcttgtat ttagcggcaa agccaccccc attgaaggcc atcacagagg ccacttactt
     1081 cgaccgtgtg gtccggtctt accacgtaag cgaaagggtt gcggactgtg ctgaatcgcc
     1141 gcgctaggaa ccaaagtgac attgtgcagc gtgtcgagga gcgttgacga attggtttgg
     1201 aaacagctcg ctttccgcgt tggcgagcac tgccttcaac ttgatggttt cggttgggga
     1261 tcaattcggt cgatgctcgc caacgcgccg gtggccaaag atctcttttg tttccggtcg
     1321 taagcttcca cgggaagttt ctgacccgtt tttatatatc tcaggatctg tggcagattg
     1381 tcttccggca cagtgaagat aaccgtgatc ggttgtaact gggtaatgac ccaccagccc
     1441 gttcgcgtcg gaaacatgca ccatattgcc gacgtccacc aagcggagtc cgacgcgccc
     1501 gcggatcggc gcattgatgc ggcaataact cagctgcagc ttccattatc gacctgcccc
     1561 tgatccgctc tcaccacacc tcgtattgac gcacaagtga ctcttgcgtg tcgagttgtt
     1621 gcttgggaac cggagtcttg ggaaaacagc acgcggtacc gttccaaatc gacttttggc
     1681 gctgatcaac aagcctgatc ccgcgctctt tgaccttcgg ctgagcgagg tggacttgaa
     1741 atggccgtgg atcgatttcc gccagcagct caccgccgga gacggtttga ccttccttaa
     1801 acagaacgtt tactagctgt ccatcgacgc ggctcttcac cgtgattgtg ttatagggcg
     1861 ctgcggaatt cgaacaaatc tgatgagtgg cttgctcgcg cacttcgcgc gccagatctt
     1921 ccggcaacaa caccggctga tacgagagca gcagagcctg ctcgatggca tgctcgagct
     1981 cgcaaatatt gcccggcagc gataagtgct tagaagcttc atagcattat cagaagtcgc
     2041 cgagatgcct ggcccggtat ggctgatttc aataggaaca ccttccggtt tgggcgcggg
     2101 cgagcacgcc tggtagaaat ttggatcgcg ccccgatcgc gccggctttt tcgcaagcat
     2161 acatggcgtt gtccaatacc ttgatcaaca cgcgttgaat ggaaccgctg ttgccgtaga
     2221 tcaccggcgc agctccacta cagttcgaag tcacctcaat cgtgcgttca tcaaaaagcg
     2281 gttgcaataa catcaatgta tcgcgcacaa gattattgat caggacggcg cgtttgggcg
     2341 ccgtccctcg ggtttggaaa agatagtatt tctcccatcc gagtaatttg cgtttcgata
     2401 tcgaaaggcg gcgcctggcg cgttccgtca gattttcttg ggaaggcata gccgagaaga
     2461 atttatcggc gtgcccacat cgtgcgcaat ggttcggcca tgcgtccgag cgtcgccagg
     2521 cgttcgacgc gcccaagctc aagctgcacg cgcaagagtt ctgtttcggc ctttttgcgc
     2581 ttgatgattt cggctctcat gctgtcattg gcttgcgcca gctcgcggtc cgttctcgaa
     2641 cgcgctgctc gagctcgtcg tgagcttagc ccaaagcctg ttcggctcgt ttacggggtt
     2701 tgtgagatac tgcttaaggc agcaatcgct gtgaacacgc ccaagcgcaa aaaagcgcgc
     2761 cagtctaacg tgatcaagta cttggagatg aaaaatattc gagacacagg gcggaaaaga
     2821 tagtggcaag aagaccggcg tcaagaccgc cataccagga actcagcatc accgcgataa
     2881 aaaacagtga gaagtggctg ggatcggcgc gcgctggatt agttcagcca aagccaagcc
     2941 ggctaggacc gtggcgacag catatcgaag caacgcgggc cgatccattg atcatggtgc
     3001 gcctacacca atcggcagaa ctccaggtca tcttggaacc cataaactat gcccgcgacg
     3061 atttgcattg tgctagccca taccatacgc ggcggcgtgg gctcgagcca acagagattt
     3121 caactttcat tccgaagaga agggctgatc tctgcagtat tgtcgtgcgt aaatcgcgaa
     3181 gttcaagtcg acgcgtgcat ggcggccaca gggggctacc gaaatgccac acggagtggg
     3241 ccttggcaca ggtgcgtcac acggtagtta tctgtgctgc ggaattgttg ctcctcccga
     3301 agcgagttgg ggtaagaaat gacaacggta tggcaggaac cggctgatct catacgctcg
     3361 ccactaaacg cccgcgtctg cgctacctcg ttttcggcgg atttgacaat tttgttgttg
     3421 atgagatccc ggacgtgcaa tgaacgtgac gtaaactagt gatttagcgc gtggttgctc
     3481 gctgaattgt tggcatgcga agtgcgacat ccggccggcg atggttcttt ctgattccgc
     3541 cgccgaaacg catccttccg aagccacaga aaccaga
//
LOCUS       DASULZ010000029         6726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1221035, whole genome shotgun sequence.
ACCESSION   DASULZ010000029 DASULZ010000000
VERSION     DASULZ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6726
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221035"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1758
                     /locus_tag="VFY96_00585"
     CDS             <1..1758
                     /locus_tag="VFY96_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEX5604977.1"
                     /translation="AVVFFFIFFGVALIGVERKQLALDVFNIAKEALSKATKFVVSLT
                     PYGVFAIAANTAGTLNVDQIARIQVYLITYIVVALLVALWVLPGLISALTPFSYREVL
                     GPTRDALITAFVAADLFIVLPILIQGCKELLERHRLVGDRTRELPDVIVPTSFNFPHT
                     GKLLSISFILFAGWFADAAVPVSDYPRLALTGLLTFFGSLNVAVPFLLDVFHIPADTF
                     QLFLATGVINSRFGSLLAAVHTVSVTLLGSAAIVGSLRFSAARIVRYFCFTFFLTALT
                     IGGLRLVFNRALAGSFAGQELVYSMRPLMTTGDARLTQQTDLDNDAAKPAGSLIETIT
                     KRGILRVGVFADRLPFVFTARDGHLVGFDVEMAQLLARDLGVKAEFIAMEEAGARVPR
                     ALAMAQVDLVMTGFPVTAARASQILFSEPYLDESLAFVIKDQLREDFSSWSAIRELGE
                     FPVTIPELPYFIDSVKARAPALKLGVVNSMKRIEEGLKNATLDAVVLPAERGSVLSLL
                     YPKYTVVVPQPDIVKIPLAYPVARRDQDFAAFLNTWIELKRRDGTIHALYGHWILGKQ
                     ASKRQPRWSIIRNVLHWVE"
     gene            2039..3175
                     /locus_tag="VFY96_00590"
     CDS             2039..3175
                     /locus_tag="VFY96_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5604978.1"
                     /translation="MTGCSKEEPPPPPPPEVTVTEAVQKDVPIYIELVGSTLGSEDVE
                     IRARVEGYLTSVNFTEGSFVKKGQLLYRIDPQPFQAALSQAQANLSTARARLDKTNND
                     VARYKPLAAEKAVSQQELDNALSAQEAARFQVTAYDSLVDKAKLDLAYTTITSPIDGL
                     IGTTQKKVGALVGRGENTLLNTISQINPILFRCAIAEAEYLRLARGAAAKSKSLEKQF
                     GVELILADGTVHPHKGRLDAVERAVDPTTGTLTGQFSFPNPERILRPSQYGKARFVID
                     LKQDAVLVPQLAVQEIQGLYSVMVVKPDATVEQRMIKVGERVGNLWIIDSGLKPGEKV
                     IVEGIQKVKPGVQVSAKLEKVKETASPAGSAPASASEPKEAKGK"
     gene            3179..6337
                     /locus_tag="VFY96_00595"
     CDS             3179..6337
                     /locus_tag="VFY96_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008465636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEX5604979.1"
                     /translation="MANFFIRRPIVAIVIAILMVLMGLFSLKGLSIEQYPFLAPPSIR
                     VTATYPGASAVAVEQSVATPIEQEVNGVDKMIYMKSSNTSDGRMQLDVNFEVGVDQDT
                     ANVLTQNRVSSAQARLPQEVVQQGVTVKKQSPSILMLISVYSPKGSYDANFLTNYCGI
                     NLRDQLLRIPGIAQVDLFGGADYGMRIWLRPDKLAKLALTPSDVISAIKEQNLQAPAG
                     RVGAAPSPKDQEFTYTVSAPGRLVNPEEFENIILRQTETGAQIRIKDVGRAELGSQNY
                     DSFGRLDGKPSGTMAVYLLPGANQLKASEAIYETLRQAKVLFPPDMDYKIVYDTTPAV
                     EASIDGIVHTFIEAIILVTLVVFIFLQNLRATIIPLLTVPVSLIGTFIFFPMLGFSVN
                     TLSMFGLVLAIGIVVDDAIVVVEAVMHHIEHGMNPKEATIQAMKEVSAPVVGIALILS
                     AVFIPVAFLGGLTGQMYKQFALTIAISVLLSAFSALSLAPALSAMLLKAHHGPARGPL
                     GKFFGWFNRTFDRTTNAYIGGARLLVRRSILTIVIVAAVAVGAGLFGGALPAGFIPEE
                     DQGIFGVNVTLPPAASLERTGNVLSQVEKILTKTEGVDSFSTIGGYGVVTSTYQPNFG
                     TIFVRMKPWEERHSDALHVRGIMANVQRQVANIPEAIIFPFNIPTISGFGASAGFNFL
                     LQDRSGTLTVQQLGDSTRQFLEAARKRPELANLFTSFDPRYPQVKVDLDREKARKLGV
                     PINEVFQALSASMGGTYVNDFNRFGRLFRVYVQSEADYRRKPNDIGDIYVRSRTTNTM
                     IPLATLINITSEGGTEITVRFNLFRSVEINGVPGRGYTSGQALAALEEVFKQTMPKEM
                     GFAYSSLSYQEKVAPPAGPTFVLAIVFVFLLLAAMYESWRLPWAVLLGSPLVALGSFF
                     GVWLMGYDNNVYVQVGNIMLIGLAAKNAILIVEFAKAKHEEGMSPEEAALESARLRFR
                     PILMTAFAFILGVVPLMIASGAGAGAQNVMGTAVFWGMLVATALGVFLIPGNFAFIEG
                     LGRKKKVAAPIVSGIPSHEGAH"
     gene            6469..>6726
                     /locus_tag="VFY96_00600"
     CDS             6469..>6726
                     /locus_tag="VFY96_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX5604980.1"
                     /translation="MLTFFLTGCALGPDYLRPKVLVPENHRGLVGTPGADSLADLPWW
                     ELFKDSVLQELTREALRNNYDLITAAARVEEARAQAGVVRSF"
BASE COUNT         1371 a         1960 c         1862 g         1533 t
ORIGIN      
        1 gccgtggttt ttttctttat ttttttcgga gtcgcgttga tcggcgtaga acgcaagcaa
       61 ctcgcgctcg acgtcttcaa catcgccaaa gaggcgcttt cgaaggcgac gaagtttgtc
      121 gtgagtttga ccccctatgg cgttttcgcg atcgccgcca acaccgccgg tactttgaac
      181 gtggaccaga tcgcgcgcat ccaggtttac ttaatcacct acattgtggt ggctctgctc
      241 gttgcactgt gggtactacc gggcctaatt tcggcgctga cgcccttcag ctatagagag
      301 gtgctcgggc cgacgcggga tgccctaatc acagcatttg ttgcagccga tttgtttatc
      361 gtcttgccga ttttgatcca gggctgcaag gaactcctcg agcggcaccg gcttgttggc
      421 gaccggacca gggaactgcc cgacgtgatc gtgccgacgt cgttcaactt tccccatact
      481 ggcaagctgc tgtcgatcag tttcatcctg ttcgccggat ggttcgctga cgccgcggtt
      541 ccggtatctg actacccgcg cctggcattg acaggtcttt taaccttctt cggcagcctg
      601 aatgtggccg tgccgttttt gttggacgtg ttccatattc ccgccgatac gtttcaactg
      661 tttcttgcca ccggtgtgat caactctcgt ttcggatctc ttttagccgc ggttcacacg
      721 gtgtccgtaa cactattggg cagcgccgcc atagttgggt cgctccgttt cagcgctgcg
      781 cgtatcgtcc gctacttttg cttcacgttc ttcctcacgg ctctgacgat tggcggtttg
      841 cgattagttt tcaaccgggc attggcggga agcttcgccg gccaagagct ggtctatagc
      901 atgcgcccac tcatgaccac tggcgatgcg cgcctgaccc aacagacgga tttggacaac
      961 gatgccgcga agcctgcggg ttcactgatc gaaaccatca ccaagcgcgg aatactccgc
     1021 gtcggggtat ttgccgatcg tctacctttt gtgttcaccg cccgagatgg ccatttagtc
     1081 ggcttcgacg tggaaatggc acagctcctt gcgcgcgatc tcggcgtcaa ggcggaattc
     1141 atcgcgatgg aagaagcggg cgcgcgggtg ccacgcgcgc tggcaatggc tcaggtcgac
     1201 ctggtgatga cgggatttcc tgtgacggcg gcgcgcgcca gccaaattct gttttccgag
     1261 ccctacctgg acgagagcct agctttcgtg attaaggatc aactgcgtga ggatttttcc
     1321 agctggagcg cgatccgaga actcggcgag tttccagtca cgattcctga gctgccatat
     1381 ttcatcgatt ccgtgaaagc gcgagcgcct gcgctgaagc tcggagtggt aaactcgatg
     1441 aagcggatcg aagagggact gaaaaatgcg actttggacg ccgtagttct tcccgcggag
     1501 cgcggttcgg tgctgagctt actttacccg aaatacaccg tcgtcgtgcc tcaaccggac
     1561 attgtcaaga taccactggc gtatccagtg gcacggcgcg atcaggactt tgcagccttt
     1621 cttaacacct ggatcgagct caagcgccga gacggcacca tccacgctct ttacggccac
     1681 tggattctgg gcaagcaagc gagcaaacgc cagccgcgct ggtcaattat tcgtaacgta
     1741 ctgcactggg tggaatgagc tagcaggttc tcctccgaga ggtgtggacc ccgtcatcat
     1801 tggaccaaag tccaatatgc acaagtttac gatttcgata ataaagttcg tgttctctga
     1861 tgtttgattt cttttgaaat caagccagca aattggctcc gcagcgatgc tggcgatgcc
     1921 gaaacgatcg taagtgcctt cgtgggagga tcatgtgaac cgtgaaattg tcaggtcgat
     1981 tcgggacggg tgtaggatgt tttcaaccgg tctcgttcta ttggtcagcc tagccgcaat
     2041 gaccggatgc tcaaaagaag aacctccccc gccaccacca ccagaagtca cagttactga
     2101 ggcggtgcaa aaagatgttc cgatctacat agagcttgtc ggcagcacgc tcggttccga
     2161 ggatgtcgag attcgcgctc gtgtagaggg ctatctcact tcggtcaact tcaccgaagg
     2221 ctcctttgtc aaaaaaggac aactgctcta cagaatcgat ccgcagccgt ttcaagcggc
     2281 gctttctcaa gcgcaggcca acctatcgac ggcgcgggct cgcctcgaca aaactaacaa
     2341 cgacgttgcg cgatacaaac cattggcggc ggagaaggcg gttagtcaac aggaactgga
     2401 caacgcgttg tccgctcaag aggcggctcg atttcaggtc acagcttacg attcgctcgt
     2461 ggacaaagcg aaactcgatc ttgcatatac cacgatcact tcgcccattg acggactcat
     2521 cgggacgacg cagaagaaag tcggcgcctt ggtcgggcgc ggtgaaaaca cgctgctcaa
     2581 tacgatttcg cagatcaatc cgattctctt ccgctgcgcg atcgccgagg cggaatattt
     2641 aagactcgct cggggcgccg ccgctaaaag taagtccctg gagaagcaat ttggcgtcga
     2701 gcttattctt gccgatggaa ccgtgcatcc gcacaaaggc cgtttggacg ccgtcgagcg
     2761 cgcggtggac ccaaccacgg gcactctgac cgggcagttt agctttccca atcctgagcg
     2821 gattcttcgg ccaagccaat acggcaaagc gcgattcgta atcgatttga agcaggatgc
     2881 cgtcctggtc ccgcaactcg ccgtgcaaga gattcaaggg ctctacagcg tcatggtcgt
     2941 aaagccggat gctacggtgg agcagcgcat gataaaagtt ggcgaacggg tcggcaatct
     3001 ctggatcatc gattcaggac ttaagccggg ggaaaaggtt atcgtggaag ggattcaaaa
     3061 agtgaagccc ggcgtgcagg tcagcgccaa actcgaaaag gttaaagaaa ccgcttcgcc
     3121 tgcagggagc gctccagcat cagccagcga acccaaggaa gcgaaaggaa aataagcgat
     3181 ggcaaacttc ttcatccgcc ggcccatcgt cgcaatcgtt attgcgatct tgatggttct
     3241 gatgggactg ttcagcctca agggtctctc gatcgagcag tacccattcc tcgcgccgcc
     3301 ttccatccgc gtcacggcca cctacccggg cgcttccgcg gtggcggtgg aacaatcggt
     3361 agcgacgccc attgagcagg aagtgaacgg cgtcgataag atgatctata tgaaatcatc
     3421 gaacaccagc gacggccgca tgcagctcga cgtcaacttt gaagtcggtg tcgaccagga
     3481 caccgcgaac gtgttgacgc aaaaccgcgt ctcttcggct caagcgcgcc taccccagga
     3541 agtcgttcag cagggtgtca ccgtcaaaaa gcagagcccg agcattctga tgctcatctc
     3601 cgtctactcg cccaaaggga gctacgacgc caactttttg accaactatt gcggcatcaa
     3661 tctccgcgat cagctgctgc gaatccccgg gatagcccag gtcgatcttt tcggcggcgc
     3721 ggattatggc atgcgcattt ggctgcgccc cgacaagttg gccaaactcg cgctcacacc
     3781 ttccgatgtg atctcggcaa tcaaagagca gaacttgcaa gctccggccg ggcgcgtcgg
     3841 cgccgcacct tcaccaaaag accaagaatt tacctatacg gtgagcgccc cgggccggct
     3901 cgtcaaccca gaggagtttg agaacatcat tctccgtcag actgaaaccg gcgcgcagat
     3961 ccgcatcaag gacgtcggac gcgcggagct gggctcgcag aattacgact cctttggacg
     4021 gctcgacggc aagccgtccg gcacgatggc cgtctacctg ctgccgggtg ccaaccagct
     4081 caaggcctcc gaagcgatct atgagaccct gcgccaggcg aaagtgctgt ttccgcccga
     4141 catggactac aagatcgttt acgatacgac gcctgcggtg gaagcttcca tcgacggtat
     4201 tgtccacacg tttatcgagg cgatcatcct ggtgacgctg gtggttttca tcttcctgca
     4261 aaacctgcgc gcgacgatca ttccgctgct caccgtgccc gtgtcgctca tcggcacctt
     4321 catttttttc ccgatgctcg gcttctcagt taacactctc tcgatgttcg gtttggtgct
     4381 ggccatcggc atcgtcgtgg acgatgccat cgtcgtggtc gaagcggtga tgcaccacat
     4441 cgagcacggg atgaacccga aggaagcgac catccaggcg atgaaggaag tctcagctcc
     4501 ggttgtcggc atcgccctca ttctttcagc cgtcttcatc ccggtcgcct tcctgggcgg
     4561 cctgaccggg cagatgtaca agcaattcgc cttgaccatc gctatttccg tgctgctctc
     4621 cgctttcagt gcactgtcac tcgcgcccgc actctcggcc atgctgctca aggcacacca
     4681 tgggccggcc agggggccgc taggcaaatt cttcggctgg ttcaaccgca cctttgaccg
     4741 caccacgaac gcatatatcg gtggcgcacg gctcctggtg cgacgttcga ttcttaccat
     4801 cgtcatcgtc gccgcggtgg ccgtcggcgc cggcctattc ggcggcgcgc tgcccgctgg
     4861 cttcatcccg gaagaagatc aaggaatctt cggcgtcaac gtcacgctcc cgcccgccgc
     4921 gtctctcgaa cgcacgggta atgtattatc acaagtcgag aagatcctga ccaagactga
     4981 aggagtcgac tctttctcga ccattggcgg ctatggcgtg gtaaccagca cctaccaacc
     5041 caacttcggt acgattttcg tccgtatgaa gccgtgggaa gagcgtcatt ccgacgcgct
     5101 ccatgtgcgc ggtatcatgg cgaatgtgca gcggcaggtg gcgaacatac ccgaggcgat
     5161 catctttcct ttcaacattc caacgatctc ggggtttggc gcgtcggccg gttttaattt
     5221 tctattgcag gaccggagtg gcacgctcac cgtgcaacag cttggcgact ccacccgcca
     5281 gtttctcgag gcggcgcgta agcggcccga actagccaat ctcttcactt ccttcgatcc
     5341 tcgctatcct caagtgaagg tagacctcga ccgcgagaag gcacgcaagc tcggcgtgcc
     5401 gatcaacgag gtctttcagg ccctgtccgc aagcatgggc ggcacctacg tgaatgactt
     5461 caaccgtttc ggccgtctgt tccgcgtcta tgtgcagtcc gaggccgact atcgccgcaa
     5521 gcccaacgac atcggcgaca tttatgtccg cagccgcacc actaacacga tgatcccgct
     5581 cgctactctc attaacatca cctccgaagg cggaaccgag atcacggttc gcttcaacct
     5641 gttccgctcc gtcgaaatta atggcgtgcc tgggcgcggt tatacttctg gccaagcgtt
     5701 ggccgctctt gaggaagttt tcaaacagac gatgccgaag gagatgggtt ttgcctattc
     5761 cagtctttcc tatcaggaaa aagtcgcgcc gcctgcggga ccgacctttg ttctcgccat
     5821 cgtattcgtg tttctcctgc tggccgctat gtatgagagt tggcgcctgc cgtgggccgt
     5881 tttgcttggc tctcctctgg ttgctttggg ttcgttcttc ggggtctggc tgatgggcta
     5941 cgacaacaac gtctacgttc aagtcggcaa catcatgctg atcggcctcg ctgccaagaa
     6001 cgcgattctg atcgtggagt tcgccaaagc gaaacacgaa gaaggcatgt cgcctgaaga
     6061 ggccgcgctg gagtcggcgc gcttgcgttt tcgtccgatc ctcatgacgg cattcgcttt
     6121 cattctcggc gtcgtgccgc tgatgattgc gagcggcgcc ggagccggtg cgcagaacgt
     6181 catgggcaca gcagttttct ggggcatgct ggtcgctacg gctctcgggg tattcctcat
     6241 tcccggcaac ttcgcattca tcgaaggcct cggcagaaag aaaaaagtcg ccgccccgat
     6301 cgtttcaggt ataccgtcgc acgagggagc ccactgatga cgcgagcttc tttgcgactc
     6361 cggacgccgg cgatgttaca caaattcatt gttagcacga agatcacgag cggcacgaag
     6421 aagttttcta ttcgtttagg tcttcgtcga ctttgtggtg aattattatt gctaactttt
     6481 tttcttaccg gatgcgccct cggtcccgat tatctgcgac caaaggtcct ggttccggaa
     6541 aaccaccgcg gacttgttgg cacacccggg gcggactctc ttgccgatct tccctggtgg
     6601 gagcttttca aagattcggt gctgcaggag ctgacccgag aggccttacg taacaattat
     6661 gacttgatca ctgctgctgc gcgcgtcgag gaggcaaggg cgcaggcggg tgtcgttcgc
     6721 tctttt
//
LOCUS       DASULZ010000030         4030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_307869, whole genome shotgun sequence.
ACCESSION   DASULZ010000030 DASULZ010000000
VERSION     DASULZ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307869"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..605
                     /locus_tag="VFY96_00605"
     CDS             117..605
                     /locus_tag="VFY96_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5604981.1"
                     /translation="MPGKADFTQEEWKKIVAAAPMVGLAVTCASPNGPWGVMKEMLSM
                     GMAMAEMLQKGSSNPLIADLAADLKARQTKPEPPQNLKDPEQCKDAALQHVRAVNELL
                     DSKVKGPQADEFKRWLVTVAHRVAEAANEGGLFGCGGERVSEAEKNIIRQIAFNLGQP
                     AN"
     gene            complement(657..>4030)
                     /gene="metH"
                     /locus_tag="VFY96_00610"
     CDS             complement(657..>4030)
                     /gene="metH"
                     /locus_tag="VFY96_00610"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX5604982.1"
                     /translation="AAKKAVELALDRQLSRPLFVAGAMGPTNRTCSISTDVQSAATRG
                     VTFDELVDGYYEQARGLLDGGVDLLLVETIFDTLNAKAAFFAIAKLFDETNRRVPLMA
                     SVTFIQPGSNRGVTGQTVEAFWNSISHVPLLSVGMNCALGPKEMRPLIEELAGIAPIY
                     VSSYPNAGLPDPLLPTGFPETPESLAPQLAEWVESGWLNIVGGCCGTTPAHIEAIAEA
                     VRGKAPRRLPQLDPYLRLSGLESLTVRPESNFVNIGERTNVTGSPVFAKLVLAGDFEK
                     AVSVARQQVEGGAQIIDVNMDEGMLDSKAAMEKFLRLIAGESDIARVPVMVDSSKWEV
                     IEAGLKCLQGKGIVNSVSLKEGEEKFIAQAKLIGRYGAAVVVMAFDERGQADTLERKI
                     EVCARSYGILVEQVGFAPQDIIFDPNILTVGTGIEEHNIYAVNFIEATRWIKQNLPLA
                     KVSGGVSNISFSFRGNNPVREAMHSAFLYHAIKAGMDMGIVNPGMLAVYEEIPKDLLE
                     LVEDVLLNRRPDATERLVSFAETVKKTDKTVARDDSWRDGPVEERLAHALVKGLTDFI
                     DQDVEEARTRYGKPLAVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKKAVAYLT
                     PFLEAEKKAAKNIDQRTKLVFATVKGDVHDIGKNIVAVVLSCNNYDVIDLGVMVPADK
                     IIDTAIEEKADMIGLSGLITPSLDEMTHVAAEMNRRGLNLPLLIGGATTSKRHTAVKI
                     APAYAHETIHVVDASRAVPVVGALSKPDARRALASQNRREQQELREQFESRTGPQLLG
                     YEAARQHRLNTHWDGQQIAVPEFIGRRVLKDFPLDELVPYIDWSPFFHTWEMRGRYPD
                     LLEDPKRGPAARELFANAQEILREILEKKLLAAHAVYGFYPANSDGDDIILYSDATRT
                     ETLTCLHTLRQQKVNSSGKPQLALADYIAPKESGRIDYIGAFAVTAGHGCQQLAERFE
                     RENDQYNAIMAKALADRLAEAFAELLHKRARNDWGYGNEERLSPEDLIAEKYRGIRPA
                     PGYPAQPDHTEKPLIFDLLDVGAATGITLTESFAMYPAASVCGLYFAHQEARYFAVSA
                     IGKDQVESYAARKGMKVSEVERWLGSILGYDPAKP"
BASE COUNT          847 a         1180 c         1119 g          884 t
ORIGIN      
        1 gccgaggaag tctcgttgac cattgccgac gattgactca ttagtttgtt gcgccgctgt
       61 ttgttctgct cgcgttatgt tagacaaacc atattcaaaa acgggaggcg atggcgatgc
      121 caggtaaagc cgactttacg caggaggaat ggaaaaaaat cgtcgcggct gcccccatgg
      181 tgggactggc ggtcacatgc gcaagtccga acggtccatg gggagtaatg aaggagatgt
      241 tgtcgatggg catggcgatg gcggaaatgc tgcaaaaggg tagctcaaat ccgctgatcg
      301 ccgatctcgc cgccgatctc aaggcgcgtc agaccaaacc ggagccgccg caaaatttga
      361 aggatcccga gcagtgcaaa gacgcggcgc tgcaacacgt gcgcgcggta aacgagctcc
      421 tcgatagcaa ggtcaaaggg ccgcaagcgg atgaattcaa gcgctggctc gtgactgtcg
      481 ctcatcgggt tgcggaagcg gctaacgaag gcggtctgtt cgggtgcggt ggcgagcggg
      541 tctctgaggc ggaaaaaaat attattcgac aaatcgcctt caatctcggg caaccggcaa
      601 attgatgcgc agctttcatc cagccgcaaa agctccgatg aacggtgaag taatttctag
      661 ggcttcgctg gatcgtagcc caaaattgac ccaagccagc gctcgacttc gctcaccttc
      721 atgcctttgc gtgcggcgta ggactccact tggtcctttc cgatcgcgct gacggcgaaa
      781 tagcgcgctt cttgatgcgc gaagtagagc ccgcaaaccg acgccgccgg atacatggcg
      841 aagctctcgg tcagtgttat tcccgtcgcg gcaccgacgt cgagcagatc gaaaatgagc
      901 ggcttctcgg tatggtcggg ctgggcggga taacctggcg ccggccggat gccacgatat
      961 ttctcggcaa tgagatcttc aggactaagc cgctcctcgt tgccgtatcc ccaatcgttg
     1021 cgcgcgcgtt tgtgtaaaag ctcggcaaaa gcttccgcca ggcgatccgc caacgctttg
     1081 gccatgatcg cattgtattg atcattctct cgctcgaagc gttcagccaa ttgctgacaa
     1141 ccatgtccgg cggtcaccgc gaatgcgccg atatagtcga tccgaccgct ttccttgggc
     1201 gcgatgtagt cagccaaggc gagctgcggt ttaccgctcg aattcacttt ctgctggcgt
     1261 agcgtatgta gacaggtcaa ggtttcggtg cgcgtcgcat cggaataaag gataatgtcg
     1321 tcgccatcgc tattcgccgg ataaaaaccg taaacggcat gagccgctaa aagctttttt
     1381 tcaaggattt cccgcagtat ttcttgtgcg ttggcgaaca attcgcgcgc cgccggtccg
     1441 cgcttgggat cctcaagcaa atctggatag cgcccgcgca tttcccaggt gtgaaaaaat
     1501 ggcgaccagt cgatatacgg caccaactca tcgagaggaa aatcctttaa cacccgccga
     1561 ccgataaact ccgggaccgc aatttgctgc ccatcccagt gggtgttcaa tctgtgctgc
     1621 cgcgctgctt cataacccag caattgaggc ccggtccggc tttcgaactg ttcgcgaagt
     1681 tcctgttgtt ctcttcggtt ttgagaagcg agcgcgcgcc gtgcatcggg cttgctaagc
     1741 gcgcccacca ctgggaccgc gcgcgaggcg tccaccacgt gaatggtctc atgcgcgtac
     1801 gccggcgcga ttttcaccgc ggtgtggcgc ttgctcgtgg tcgcaccacc gatcaagagc
     1861 gggagattca aaccgcgccg gttcatctcc gccgccacat gggtcatctc gtcgagagac
     1921 ggagtgatca gcccgctcag gccgatcata tccgcttttt cttcaatcgc cgtatcaatg
     1981 atcttatcgg ccggcaccat cacgccgagg tcgatcacgt cgtaattgtt gcagctgagc
     2041 acgacagcaa caatattttt gccgatgtca tgcacgtcgc ctttgaccgt agcaaatacc
     2101 aatttggtgc gctgatcgat gttcttcgcg gcttttttct ccgcctcgag aaacggcgta
     2161 agataagcca cggctttttt catcacccga gcgcttttta ccacttgagg caaaaacatt
     2221 tttccggagc cgaacagatc accgacgaca ttcatgccat ccatcagcgg cccttcgatc
     2281 accgccagag gcttgccgta ccgggttctt gcctcttcca catcttgatc gataaaatcg
     2341 gtgagcccct ttaccagagc gtgcgccaat cgctcttcta ccggtccatc gcgccagctg
     2401 tcgtctcgcg caacggtttt atcggttttc tttaccgtct cggcgaagga aacgagtcgc
     2461 tccgtcgcat cgggacgccg gttgagcagc acgtcttcga cgagttccag taagtccttg
     2521 ggaatttctt cgtagaccgc gagcatgccg ggattgacaa tgcccatgtc catccccgcc
     2581 ttgatcgcat gatacaaaaa cgccgagtgc atcgcctcgc gcaccggatt attgccgcga
     2641 aaggaaaacg aaatattgct gacgccgccg ctgaccttcg caagcggcag attctgcttg
     2701 atccagcggg tcgcttcgat gaaattgacg gcgtagatgt tatgctcttc gataccggtg
     2761 ccgaccgtga gaatgttggg atcgaagata atgtcctgtg gcgcaaaccc gacctgctcg
     2821 acgagaatcc cgtaagagcg cgcgcaaacc tcgatttttc gctcgagcgt atccgcttga
     2881 ccgcgctcat cgaacgccat cacgacaacc gccgcgccgt agcggccgat cagcttggcc
     2941 tgagcgataa atttctcctc gccttctttg aggctgaccg aattgacgat acccttcccc
     3001 tgcaaacact taagccccgc ttcgatgact tcccatttcg agctatcgac catcaccggt
     3061 acgcgcgcga tatccgattc gccagcgatg agcctgagaa atttctccat tgcggctttg
     3121 gaatcgagca ttccttcatc catgttgacg tcgataatct gcgcgccccc ctcgacttgc
     3181 tggcgcgcca cgctcaccgc tttttcaaag tcgccggcaa gaacgagctt ggcgaagact
     3241 ggcgaaccgg tgacattggt tcgctcgccg atgttgacga aattggactc gggtcgcacg
     3301 gtgagacttt ccaggccgct caagcgcagg tagggatcga gttgtggtaa gcggcgcggc
     3361 gccttgccgc ggaccgcctc cgcgatcgct tcgatatgcg ccggcgtggt gccgcagcaa
     3421 cctccaacaa tattgagcca gccgctttcc acccactccg ccaattgcgg tgccagtgat
     3481 tcaggcgtct cgggaaaacc ggtgggcaag agcggatcag gcaatcccgc gttgggatag
     3541 ctgctcacat aaatcggtgc gatgccggcg agttcctcga tgagcggtcg catttccttg
     3601 gggccgaggg cgcagttcat gccgacactc aacagcggca cgtgcgaaat cgaattccag
     3661 aatgcctcca ccgtttgccc cgtgacaccg cggttgctgc ccggctgtat gaaagtcacc
     3721 gacgccatca acggaacgcg cctgttcgtc tcatcgaaaa gcttggcgat cgcgaagaat
     3781 gccgctttgg cattgagcgt gtcgaaaatg gtttcgacca acagcagatc gactccgcca
     3841 tccagcaaac cgcgcgcttg ctcgtaatag ccgtcgacca actcgtcgaa ggttacgcca
     3901 cgggtggcgg cgctttgtac atcggtcgaa atcgagcagg tgcgattggt gggacccatg
     3961 gctccggcga cgaaaagggg gcgagacagt tgcctatcga gtgccagctc gacggctttt
     4021 ttggcggcct
//
LOCUS       DASULZ010000031         2184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_206341, whole genome shotgun sequence.
ACCESSION   DASULZ010000031 DASULZ010000000
VERSION     DASULZ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206341"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="VFY96_00615"
     CDS             complement(<1..893)
                     /locus_tag="VFY96_00615"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5604983.1"
                     /translation="MHYRRPLMLLSLLIVFCAPVSAPAQEKLRLAWAGFSPTNSPIWT
                     IEDRKLLQKQGVQPEIIAINNSPTVLQALLADEIDVASISVTTLTSSRLAGADTVMIV
                     GVVPTFVDHIISVSSITAVEQLKGKTGGVNRLGTTSDLGLRLALSKLGIEPDKDVKII
                     PTGGTAERFAALSKGITQFTIIPEPFVTQAEKLGLRNLFNISDLKIPFWWNGILSREN
                     IVKSKRPLMLKLVRAMIEAIHIIKTEKEYAKALFKKNLGVADPEGLDRAYRDYSNAFP
                     EVPYPKADGVKTMLDYLSKTNP"
     gene            1168..2139
                     /locus_tag="VFY96_00620"
     CDS             1168..2139
                     /locus_tag="VFY96_00620"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5604984.1"
                     /translation="MASKGILVTGGSGLVGAYAVTMLLDRGERPVIYDVAVNERLLEA
                     VGVESGKVTAIRGDMMDLPALISVLRDHDCDRIIHLAAFLGEEVQRRPYSGVRLNFMG
                     TVNVFEAARLERVSRIIFPSSGTVYLGSLGEGLHKIDETIPMNPPSIYAATKASCEFM
                     GRAYAKRYGFEFICLRYTGGLYGPSPAALKATREIAIQQMIRAAVKGEPAKIGWPYGP
                     AEILYGKDAAKGTVLAVLKDKFADTLFHIGNGITLSGDDIVAGVRKAYPGSNIELVKE
                     KNPMPYPDSRLASDFSRAKDQLGYEPDYSIEKAVLDYGATLKRLEHL"
BASE COUNT          507 a          565 c          604 g          508 t
ORIGIN      
        1 ggattcgtct tcgataagta gtcaagcata gtctttaccc catcagcttt cgggtaaggc
       61 acctccggaa aagcattgga ataatctcta taggcccgat ccaaaccctc cgggtccgca
      121 acacctagat ttttcttgaa cagagctttc gcgtattcct tttcggtctt gatgatgtgg
      181 atcgcttcga tcatcgcgcg gacaagcttc agcatcaacg gccgttttga tttaacgatg
      241 ttctcccggg agagaatgcc gttccaccag aagggaattt tcaggtcgct gatattaaaa
      301 agattacgca agccaagctt ctctgcttgg gttacaaacg gctcgggtat aatcgtaaac
      361 tgcgttattc cctttgagag cgcggcgaaa cgctctgcgg tccctcccgt gggaatgatt
      421 ttcacatcct tgtcgggctc gatacccagt ttactgagag cgagtctgag tcccagatcc
      481 gaggttgtcc cgaggcggtt gacgccgccc gtcttgccct taagttgttc cactgccgtg
      541 atgctcgaca ctgaaataat atggtcgacg aacgtcggaa ccacacccac gatcattacc
      601 gtgtcggcgc cagccaaacg cgagctagtc aatgtggtga ccgagatact tgccacatcg
      661 atctcgtcgg caagaagagc ttgaagaacc gtgggactat tgttgatggc aatgatttcg
      721 ggttgcacgc cctgtttctg gaggagcttt cggtcttcaa tggtccagat tgggctattg
      781 gtcgggctga agccggccca tgccaaccgc aatttttctt gcgcgggcgc ggaaacgggc
      841 gcgcaaaaca caataagcag tgatagaagc atcagcggtc gtcggtaatg catgtcagtc
      901 ctcctcgcta agtctattcg tgccggctga catcatgctt gaaaaaaaag tgaggtcaag
      961 cccgggtggc cgtcaacaag aacagaagcc gatggactgg cgttcggcga tcagtctatc
     1021 cacgcccagg ctaatcacga ttccaagaaa gattcccgct tcgcttggaa tgacactatc
     1081 gcgttttctc gccaatcgaa gcgccctgcg ttgactcaaa gaggggtttg agatactaga
     1141 ggcaccgttc cgagaagaag agattatatg gcgagcaaag gaatactcgt aactggcggt
     1201 tccggactgg tcggagcgta cgccgtaaca atgttgctcg atcgcggcga gcggccggtc
     1261 atctatgatg ttgccgtgaa cgagcgactg ctcgaggcgg tcggtgtcga gagcggcaaa
     1321 gtcacagcga tccgtggcga tatgatggac ttgcccgcgc tgatttccgt tctacgagat
     1381 catgattgtg atcggatcat tcatcttgcc gcttttctgg gcgaagaagt acaacgccga
     1441 ccgtacagcg gcgtgcggct gaatttcatg ggaaccgtca atgtttttga agccgcgcgt
     1501 ctggaaagag tgtcccggat catatttcca agctcgggaa ccgtctactt ggggtcgctc
     1561 ggcgagggtt tgcacaaaat cgatgagaca attccgatga atccgccgtc gatttatgct
     1621 gcgaccaaag cgagctgtga attcatgggc cgtgcctacg ccaaacgcta cgggttcgaa
     1681 tttatttgcc tgcgttacac gggtgggctg tatggtccct cgccggccgc gcttaaagcg
     1741 actcgcgaaa tcgcgatcca gcagatgatt cgcgcggctg tgaaaggcga gccggcaaaa
     1801 atcggctggc cttatggacc ggcggagatt ctctacggga aggatgcggc caaaggcacc
     1861 gtattggcgg tgctcaagga caagttcgct gacacgctgt ttcacatcgg caatgggatc
     1921 acactcagcg gcgatgacat tgtcgcaggg gtgcgcaaag cttatccagg atcgaacatc
     1981 gagctggtca aagaaaagaa tcccatgcct tatcccgatt cccgacttgc cagcgatttt
     2041 tctcgcgcga aagaccagct cggttatgag cccgattatt ccatcgaaaa ggcagtcctg
     2101 gactacggcg caacgctgaa gagactggag catctttaaa agccgcttcc gaagtgaagt
     2161 tctctctgcc gactacgtct aaag
//
LOCUS       DASULZ010000032         6400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_713955, whole genome shotgun sequence.
ACCESSION   DASULZ010000032 DASULZ010000000
VERSION     DASULZ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6400
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713955"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VFY96_00625"
     CDS             complement(<1..251)
                     /locus_tag="VFY96_00625"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5604985.1"
                     /translation="MALIKLTGIVPRMIQYGGGISAVRTLPKAKGKRPAVIILHERYG
                     IDQHTKDLAVKLAQAGFVGLAPDLFHRFTGDRKAVLRGE"
     gene            373..690
                     /locus_tag="VFY96_00630"
     CDS             373..690
                     /locus_tag="VFY96_00630"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX5604986.1"
                     /translation="MDLKDGASIVADLTTIGRKSGLPRTVELRFIYHQGKFYASSSRV
                     KGKHWCENLLNNPAVVITLKGQKIAGLARQINDDKLRREILATRHSSRELDRVVFEIK
                     PPG"
     gene            1137..1877
                     /locus_tag="VFY96_00635"
     CDS             1137..1877
                     /locus_tag="VFY96_00635"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEX5604987.1"
                     /translation="METGLAVTDVGLWRDLQKGVAIRTVVLERSQPNYTVELKLARFN
                     SQLISARVLDSGEFQLRSASAKTFAEKSQAIAAINANYFDERGRPLAYLKTRVKEVNR
                     SLSKHSLYTGIFGIDESGAFVTHRDEFAPAQASEALQSGPLLLHRGLPIAINAALGRY
                     ARRAVIGIDNTGAIIIAVADAVLGGLALAELQKLFVDNRWHIDAVELLNLDGGGSAQL
                     YVKSGKFEESLPGTSEVPVAIGFFNKPR"
     gene            complement(1964..3406)
                     /locus_tag="VFY96_00640"
     CDS             complement(1964..3406)
                     /locus_tag="VFY96_00640"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX5604988.1"
                     /translation="MNVNFLPLLPVVQVLLTALVVLIRDLFLSERDSKGYLAFISLLG
                     IALAVAESLALWGGQQGAFDDTLMLDNFALYFSLIFLLTAALTILTSIQYLRLTAIHE
                     GEFYALVLFATVGMMIMAAANDLLVFFLGLETMSIAVYVLTGMWRASERASEAAMKYF
                     FMGAFATGFLLYGIALIYGATGSTHLNVIAEALVEQPSEWPAYLIGGGLLLLVGFAFK
                     VGAVPFHFWIPDVYEGAPTPVTGFMAVAVKAAAFAAWTRVLLHKLSALDSDWVFPLWL
                     IAIGTMTLGNLLALTQSSVKRMLAYSSIAHAGYLLIAIVVGEEWGGLPLLFYVASYAL
                     MTVGAFAVLTSVADADGPNENYGSFAGLGFKRPFIGLAMSIFMLSLAGFPPLAGFVGK
                     FYVFRGAVLSGYIGLAIVGVLNSLLSVVYYLRVIVAMYMEDGGVEGKSFRQAPYVYIA
                     IAFALVGTIYLGVLPASALDWSRTAFASLE"
     gene            complement(3396..4946)
                     /locus_tag="VFY96_00645"
     CDS             complement(3396..4946)
                     /locus_tag="VFY96_00645"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX5604989.1"
                     /translation="MFGLPIGHISILLLIPVLGAGLLLFVPRRQTQTLFSLATLASGL
                     DFLWSVKIFALFDGASGEMQLVERAPWMPGFGIQYLVGIDGISLFLVLLTTLLMPVAI
                     LASWSVKERVKEYLIFMLLLETGMLGAFVALDLFLFYVFWEVMLVPMYFLIGVWGGTR
                     RIYAALKFVVYTMSGSLLMLVAIIYLATRHAQVEQALTFDVFKLYGLHLPFDQQIWLF
                     LAFALSFAIKVPLFPFHTWLPDAHVEAPTAGSVILAGVLLKLGTYGFLRFAMPLFPDA
                     AMAAAPIIVALAVIGIIYGALVAMMQSDIKKLVAYSSVSHLGFVMLGLFAFNIQGIQG
                     SVYQMLNHGLSTGALFLLVGMIYDRRHTRLISEFGGLWKQLPVCSAMLMVITLSSIGL
                     PGLNGFIGEFLILLGSFSARPLWTAVATTGVILGAIYMLWMYRRVIFGPLVNAANEKL
                     SDLNSREIGLLVPILFFVVLMGIYPTPFLHRMAPAVEVTLKRIQSAVAAPVALNHRTG
                     MTPTTHER"
     gene            complement(4952..>6400)
                     /gene="nuoL"
                     /locus_tag="VFY96_00650"
     CDS             complement(4952..>6400)
                     /gene="nuoL"
                     /locus_tag="VFY96_00650"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX5604990.1"
                     /translation="YLLIGFWYRDVNNTIAGNKAFIVNRIGDFGFILGIFLLITGLGR
                     QGIWTLDFIELQKHTQLLSPAMIGAITLLLFVGATGKSAQIPLFVWLPDAMAGPTPVS
                     ALIHAATMVTAGVYMTARLHFLFALAPATLSLIAWIGAATAFFAATIALTQNDIKKVL
                     AYSTISQLGYMFLAVGMAAFTAAIFHVFTHAFFKACLFLGSGSVIHGLDGEQDMRKMG
                     GLRSRMPTTYWTYLIATLAIAGAPFTAGFFSKDLILWEAFSRGAMILWGIGLITAGMT
                     AFYMFRQLFLVFHGEFRGDDHAQAHLHESPAVMTFPLVILAIGSIFTGWVGAPEYLWG
                     SRWEHWLQPMFGAVAAQESSVDTEILVTVLTLAVVAMGIYWAYAKYCRAPAKGALSAA
                     EQGFLYRLSLNKYYIDEIYDHLIVRPFTACSRFFAEFIDPRVIDGAVNGVAAIARGLS
                     WVWCGLQTGNVQHYATGLLVGTLALLAYFLGQ"
BASE COUNT         1636 a         1836 c         1722 g         1206 t
ORIGIN      
        1 tgttcgccgc gcagcacggc tttacgatcg cccgtgaaac gatgaaacaa atcgggcgcc
       61 aagccgacga aacccgcttg agctaatttt acggctaggt ctttggtatg ctgatcgatt
      121 ccataccgct cgtggagaat aatgacggct ggccgcttgc cttttgcttt cggcaacgtg
      181 cgcactgcgc tgatgccgcc gccgtattga atcatccgcg gcacaattcc cgtcaatttg
      241 ataagcgcca ttcaaccccc acccgcaaca caaagtcctt gttgtgcggg caagcttatc
      301 catccggcac gcggctcgtc aagcaaacaa gagctggttg cccaagctcg gtcatctctt
      361 tatatttgcg gcatggattt gaaggacggc gcgagcatcg tcgcggattt gacgactatt
      421 ggacggaaga gcggtttacc gcgcaccgtt gagctgcgtt tcatttatca tcagggaaaa
      481 ttctacgcat catcgagccg agtcaaaggc aaacattggt gcgaaaacct gctcaacaat
      541 cccgccgtgg tgatcacctt gaagggacaa aaaatcgccg gtctcgctcg tcaaattaac
      601 gatgacaagc tacgcagaga aatacttgcc acccgacatt cgtcacgcga gctggaccgg
      661 gtggtgttcg aaattaagcc gcccggatag ccagcgctcg ctgaatggac caagcctatt
      721 tctggctggc atccttttga tcggtcagtt tcttctcgat attttcaaca cctttttgca
      781 tgccttgcat catccctttt tctttttgca taccttcctg aaggactttt tgtacttctt
      841 gatcccgcgc cgcactgtcc ttcttgtcgc cgcacgccga aaatcgcgag cagcgcgagc
      901 attacgattg gaccgattag cttcatgatg acgacctccg tcacctggct gctcctgtag
      961 taaccgctat tgcgacggac cgccaaccgg ctgcgcgcag taagattttt attcgcgtcc
     1021 tcgtctgtta tccttaggca atcatgcacc ggcaattgct ttgcatcgtc ttaactttcc
     1081 ttttcgtcat cctatccggc gccgcagttc aaccccaagg atcgaggaaa agccaagtgg
     1141 agaccggcct tgccgtaacg gatgtcggac tctggcgcga tctacaaaag ggtgtcgcaa
     1201 tccgcacggt tgtgctggaa cgctcccagc ccaattacac tgtcgagctg aagctggcgc
     1261 gcttcaacag ccagctgatc tcagcgcgcg tcctcgattc cggagaattt cagttgcgca
     1321 gtgctagcgc caaaaccttc gcggaaaaga gccaggcgat tgccgcgatc aacgccaact
     1381 acttcgacga aagagggcga ccgttggcat acctcaagac gagggtcaaa gaagtcaacc
     1441 ggagtctttc gaagcactct ctctatacgg ggattttcgg catcgatgag tcgggcgcat
     1501 tcgtcactca tcgcgacgag tttgcgccgg cgcaagccag cgaagccttg caatcgggtc
     1561 cgctgttgtt gcatcgcggt cttccgatcg ccattaatgc cgctctcggc cgctatgcgc
     1621 gccgtgccgt cattggcata gacaacaccg gcgcgatcat tattgctgtc gccgatgcgg
     1681 tgcttggcgg gctcgccctt gccgagttgc aaaaattatt cgtcgacaac cggtggcata
     1741 tcgatgccgt agagttgctc aatctggacg gcggcggctc cgctcaactc tacgtcaagt
     1801 cgggaaaatt cgaggaatcg ttgcccggca cctcggaagt tccggtggca atcggttttt
     1861 tcaacaaacc caggtagaca gagacacaga gttcgcggag cattttttaa gtccttcctc
     1921 tgtgttctca gtgcctccgt ggtgagtcca atttatcgct gattcattcc agcgaggcga
     1981 atgcggttcg gctccagtcg agggctgaag ccggcagcac tccgagatag atggttccca
     2041 cgagagcgaa cgcgatcgca atatagacgt agggcgcctg gcgaaagctc ttgccctcca
     2101 ctccgccgtc ttccatgtac atcgcgacga tcacccgaag ataatagacc accgaaagca
     2161 agctattgag cacgcccacg attgccagcc cgatatagcc ggacaacacg gcgccgcgaa
     2221 agacgtaaaa cttaccgacg aagccggcaa gcggcggaaa cccggcgagg gacaacataa
     2281 aaatcgacat cgccaggcca atgaatggcc gtttgaatcc cagaccggcg aagctgccat
     2341 aattttcatt gggaccatcg gcatcagcga cagaggtcaa gacggcaaac gcaccgacgg
     2401 tcatgagagc ataactggcc acatagaaca gcagcggcaa gccgccccat tcttcgccga
     2461 caacgattgc aatcaacaaa tatccagcat gggcgatgct cgaataggca agcatccgct
     2521 tgacgctcga ttgcgtcagc gccagcaaat tgccgagtgt catggtgccg atggcgatca
     2581 gccataaagg aaaaacccaa tcgctatcga gcgccgaaag cttatgcaac aacacgcgcg
     2641 tccaggccgc aaatgcagcg gctttcacgg caacggccat gaaaccggtg accggcgtcg
     2701 gcgccccttc ataaacatcg gggatccaga agtgaaacgg cacggcgccg actttaaagg
     2761 caaagccgac caacaaaagc aacccgccgc cgatcagata tgccggccat tcgctcggtt
     2821 gctccaccag cgcctcggcg atcacattga gatgagttga gccggtggcg ccataaatca
     2881 gcgcgatccc gtagagtaaa aagccggtgg caaaggcgcc catgaagaaa tatttcatag
     2941 cggcttcgct ggcgcgctcg ctggcgcgcc acatcccggt caatacgtaa accgcgatgg
     3001 acatggtttc gaggccaaga aaaaaaacca gcaggtcatt ggcggcagcc atgatcatca
     3061 tgccgaccgt ggcaaaaagc accagcgcgt agaattctcc ctcgtggatc gccgtaagcc
     3121 ggagatactg gatcgatgtc aggatcgtga gtgcggctgt cagcaaaaag atcagcgaaa
     3181 aataaagcgc gaaattatcc aacattaggg tgtcatcgaa ggcgccctgt tggccgcccc
     3241 agagcgccag cgactcggcg accgcgaggg cgatgccgag caaactaata aaggccagat
     3301 agcccttgga gtcgcgctcg ctgaggaaca aatcgcgaat caggacaacg agtgcggtca
     3361 gcagcacttg aaccaccggc aaaagcggca ggaagttaac gttcatgggt tgtaggagtc
     3421 atcccggtcc tgtgatttaa ggcgaccggc gcagcgaccg cactctggat tcttttcaaa
     3481 gtcacttcca ccgccggggc catccgatgc agaaatggcg tcgggtagat gcccatcagc
     3541 acgacgaaaa atagaatcgg cacaaggaga ccgatctccc gggaattcaa gtcgcttaat
     3601 ttttcatttg cagcgttgac cagcggcccg aagataaccc tccggtacat ccagagcata
     3661 taaatggcgc cgagaatcac acccgtggta gcgacagccg tccaaagcgg ccgcgcgctg
     3721 aaagatccga gcaaaatcag aaattcgccg ataaagccat tcaaccccgg caagccgatc
     3781 gaagacaagg tgatgaccat cagcatggcg gaacaaaccg gcagctgttt ccagagaccg
     3841 ccaaattcac tgatcaaccg ggtatggcgc cggtcataga tcatgccgac caaaagaaat
     3901 agcgcgccgg tggaaagccc atggttgagc atttgataca cactgccctg gattccctgt
     3961 atgttgaacg cgaacaggcc gagcatcacg aaaccgagat ggctgaccga agaataggca
     4021 accaatttct taatgtcgct ctgcatcatc gccacaagag cgccatagat tatgccgatc
     4081 accgcgagtg caacgatgat tggcgccgcg gccatcgctg cgtcgggaaa aagcggcatg
     4141 gcgaaacgaa gaaagccgta agtcccgagc ttcagcaaaa caccggctaa aatgacggag
     4201 cctgcggtcg gcgcttcgac gtgcgcgtcg ggcaaccacg tatggaaggg aaacaaaggc
     4261 actttgatcg cgaaggacag cgcgaaggcg agaaacagcc agatctgctg atcgaacggc
     4321 aggtgcaggc cgtaaagctt gaagacatcg aaggtcagtg cctgttccac ctgcgcatgg
     4381 cgagtcgcca gataaataat cgcgaccagc atgagcaaac tgccgctcat cgtatagacg
     4441 acaaacttta gcgccgcata aatccgccgc gtcccgcccc aaacgccgat caaaaagtac
     4501 atcggcacga gcatgacttc ccagaagaca tagaagagaa acaaatccag cgcgacaaat
     4561 gcgccgagca ttccggtttc gagaagcagc atgaagatca agtactcttt gacccgctct
     4621 ttcaccgacc aggacgcgag gatggcaacc ggcatcagca aggtcgtgag caagaccagg
     4681 aacaagctaa tcccgtcgat gccgaccaga tattgaatgc cgaagccggg catccatggc
     4741 gcgcgttcga caagctgcat ctcaccgctg gcgccgtcga agagcgcaaa gattttgacc
     4801 gaccacaaaa aatccagtcc gctggcgagg gttgccaggc tgaagagcgt ctgggtttga
     4861 cggcgcggca caaacaggag caggcccgct ccaagcaccg gaatcagcag caagatcgag
     4921 atatgaccga tcggcaaacc gaacataatc actactgacc gagaaaataa gccaacaacg
     4981 caagcgttcc caccaacagc cccgtcgcgt agtgttgcac gttgccggtc tgcagcccgc
     5041 accacaccca gctaagaccg cgtgcaatcg cggcaacgcc gtttactgca ccgtcaatca
     5101 cgcggggatc gataaactcg gcaaaaaatc gcgagcaggc cgtgaagggt cgaacgatca
     5161 agtgatcgta aatttcatcg atgtaatatt tgttgagcga gagccgatac aaaaacccct
     5221 gctcagcggc acttagcgct cccttggcag gcgcgcggca gtatttcgcg taagcccaat
     5281 agattcccat cgcaaccacc gcaagcgtca gtacggtaac gagaatctct gtgtccaccg
     5341 agctttcctg tgctgctacg gcgccgaaca tgggttgaag ccagtgctcc caacggctgc
     5401 cccacaaata ctccggagcg ccgacccatc cggtgaagat cgagccgatc gccagaatca
     5461 ccagtggaaa cgtcatgacc gctggcgatt catgaaggtg cgcctgggca tgatcgtcgc
     5521 cgcggaattc gccatggaaa accaagaaga gctggcggaa catgtaaaaa gccgtcattc
     5581 ctgcggtgat caaaccgatc ccccaaagaa tcatcgcgcc gcggctgaag gcctcccata
     5641 agattaaatc cttggagaaa aatccggcag tgaacggtgc tccggcgatg gccaatgtcg
     5701 cgatcaagta ggtccagtac gtggtcggca ttcggctcct gagcccgccc atcttgcgca
     5761 tgtcctgttc gccatccaga ccgtgaatga cgctgccgga accgagaaag agacaggctt
     5821 tgaagaaggc gtgcgtgaag acatgaaaga tcgcggcagt gaacgctgcc atccccacgg
     5881 cgaggaacat gtaacccaac tggctgatgg tcgaataagc gaggaccttt ttgatgtcgt
     5941 tttgcgtcaa cgcgatggtc gcggcgaaaa atgcggtcgc cgcgccgatc caggcgatca
     6001 gcgatagtgt tgccggcgcg agggcaaaca aaaaatgcaa tcgcgcggtc atgtaaactc
     6061 ccgcggtcac catggtggcc gcgtgaatca atgcgctcac cggcgtcggc ccggccatgg
     6121 catccggcag ccagacaaag agcgggatct gcgcggattt gccggtggcg ccgacaaaaa
     6181 gcagcaacgt gatcgcaccg atcatagccg gactgaggag ctgggtatgt ttttgcaatt
     6241 cgataaaatc gagcgtccag ataccctggc ggcccaggcc ggtgatcagc aaaaaaatcc
     6301 ccaaaatgaa accaaagtcg ccgatgcggt tcacgataaa agctttatta ccggcgatgg
     6361 tattattgac atcgcgatac cagaacccga tcaggaggta
//
LOCUS       DASULZ010000033         2783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_511199, whole genome shotgun sequence.
ACCESSION   DASULZ010000033 DASULZ010000000
VERSION     DASULZ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511199"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="VFY96_00655"
     CDS             <1..1124
                     /locus_tag="VFY96_00655"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX5604991.1"
                     /translation="AEYLQIPYAKVALAALVPALLYYVALFIEVDLLAARDGIHGLPR
                     DERPKLIPIMRRSPTFLVPLAVLVGWMFFLKRQPEEAGLVAVVAGLVTGFLTPGLKLG
                     GRELKEILVTTGRGLLDITVTTGLAGVVIGILQVTGLGFSLTFVMLSLGQASVFLLLV
                     LTAIVSIILGMGLPTTAVYILLAVLVAPGLAQMGILPLAAHLFIFYFGMLSMVTPPVC
                     LAAYAAASIGKTDPMRTGWEGMRLCAIAYVVPFLFVFSPALLLIGSWYEVTLSVLTAI
                     AGAVLLGIGLVGYLFRVIGATRRILFTASALGLLIPIVDSGHHATLTWTSNSIGLLLG
                     IFLVCLEWFARHHATPSPSAVSEDIDASTSSSRRGSETP"
     gene            1276..1650
                     /locus_tag="VFY96_00660"
     CDS             1276..1650
                     /locus_tag="VFY96_00660"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5604992.1"
                     /translation="MRILKINELQEKPMDTATPIAGWSGGPVSRTRQAIIGEGQSDNF
                     RCNVVNFRVGATTGWHVHDCDQILVVTAGKGKVATESEERDITVGDVVHIKAGENHWH
                     GATADSPMSHITVTLAGSTSKH"
     gene            1804..2676
                     /locus_tag="VFY96_00665"
     CDS             1804..2676
                     /locus_tag="VFY96_00665"
                     /inference="COORDINATES: protein motif:HMM:NF024877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5604993.1"
                     /translation="MADPVLAEMVHARGATESRHYAAVKPVIQAYRASWVKMAPYRDG
                     PVNSRRVKVDDPAAMTAQIKAKATALGANLVGICRLQPHMIDLGSEVPHEFVIACCVA
                     EGYDKVLQGPDAVEEEAMRTYAKCTEIATYLAAYVRDMGYPAIAHHNGASEVQAIPIF
                     YQVGFGELGRHGSLINEKYGASFRPGFVTTDLPLIEDQPREFGVQDFCMNCNVCQLNC
                     PGDAIPQDHVMTHGIRRWLIDLEKCYPYSRLRDEYCHLCVDVCPYSVKSHPETYKAFM
                     KERKKVGYKTPKTY"
BASE COUNT          590 a          729 c          837 g          627 t
ORIGIN      
        1 tcgccgaata cctgcagatt ccctatgcga aggtcgcgct cgcagcgctg gtgccggcgc
       61 tactctacta cgtcgccctg ttcatcgaag tggatcttct ggctgctcga gacgggattc
      121 acggtctgcc gcgcgacgaa cggccgaaat tgattccgat catgcgcagg tcgccgacct
      181 ttttggtgcc actggcagtc ctcgtgggat ggatgttttt tctcaagcgc caacccgaag
      241 aagccggatt agtggcggtg gttgcaggac ttgttaccgg ttttttaacg ccgggcttaa
      301 agctcggcgg gcgcgagctt aaagagatac tggttacgac cggccgcgga ttactcgata
      361 tcacggtcac caccgggctt gcaggagtag tcatcggcat tctacaggtg acgggattgg
      421 gatttagcct gactttcgta atgctctcgc tcggtcaagc gagcgtcttt ttacttctgg
      481 tgcttaccgc gatcgtcagc attatcctcg gcatgggtct gccgacgacc gcagtctata
      541 ttctgttggc ggtgctcgtt gcgccgggtt tggcgcaaat ggggattttg cctctcgcag
      601 cccatctctt tattttttac ttcggcatgc tgagtatggt cacgccgccg gtatgcctcg
      661 ccgcgtacgc cgcggcctcc atcggcaaga ccgatccgat gcgcactggt tgggaaggca
      721 tgcggctctg tgcgatcgcc tatgtcgttc cctttctgtt cgtcttctcg ccggcgctgt
      781 tattgatcgg aagctggtat gaagtgacgc tctcggtgtt gaccgcgatc gcgggtgcgg
      841 tcttgctcgg gatcggattg gtgggctatc tgttccgtgt catcggcgcg acccggcgga
      901 tactcttcac ggcctcggcg ctaggactgc tcataccgat tgtagacagc ggtcatcatg
      961 cgactctcac ctggaccagc aacagtatcg gtcttttgct gggaatcttt ctggtgtgtt
     1021 tggagtggtt cgcacgccat catgccacgc catcgccgtc ggctgtttct gaagacattg
     1081 acgcatcaac gagcagctct cggagaggca gcgaaacccc ttgagcgggt ttggtctctt
     1141 cgccagcaag acacaggcga aatcatcatc aaaacggaga gcgagtttcg actaccaacc
     1201 gatatcaaga agtaattcac ccgacgcggg gagcgatggg aatagagtca gtgacaatcg
     1261 ttacgaggag gtaagatgcg catactcaaa atcaatgaac tccaagaaaa gccgatggat
     1321 acggcgacgc cgatcgcggg atggagtggc ggtcccgtga gccggacgcg gcaggcgatt
     1381 atcggtgagg gacaatcgga taatttccgc tgcaacgtgg tcaatttccg tgtcggcgcg
     1441 accaccggct ggcatgttca tgattgcgat cagattttgg tggtcaccgc gggaaaggga
     1501 aaagtcgcga cagaatctga agagcgcgac attaccgtcg gcgacgtggt tcatatcaaa
     1561 gccggtgaaa accactggca cggtgcaacg gcggattccc cgatgtcgca cattacggtg
     1621 acattggcag gaagtacatc gaagcactaa attgcgttgt gcattaatgg aaacggacta
     1681 agagattgcc gcgtaggcac ggagggcacc gagatttttg attaaagata ttccgaactc
     1741 tgcgacctct attggctctg tggtgaatcg atgcgcggaa taacgcctcg acgagagcag
     1801 gggatggccg atccggtgct ggccgaaatg gttcacgcca ggggggcgac tgagtcgcgc
     1861 cactacgcgg cggtgaagcc ggtcatacaa gcctatcgcg caagctgggt gaaaatggcg
     1921 ccttatcgcg atggcccggt gaattcccga cgggtgaagg tcgacgatcc ggcggcgatg
     1981 accgcgcaga tcaaagcaaa ggccacggcg ttgggcgcaa acctggtcgg gatctgccgt
     2041 ttgcagccgc atatgatcga tcttggctcg gaagtgccac acgagttcgt gatcgcctgc
     2101 tgcgtcgccg aaggttacga caaggttctc caaggacccg acgcggtgga agaagaggcg
     2161 atgaggactt atgcgaagtg caccgaaatc gccacgtacc ttgctgccta cgtgcgcgac
     2221 atggggtatc cggcgatcgc ccaccacaac ggcgccagcg aggttcaagc gattccgatt
     2281 ttttaccagg ttggcttcgg cgagctggga cgccacggca gcttaattaa cgagaaatac
     2341 ggcgcgagtt ttcggccggg ttttgtcact acggatctgc cgctcatcga ggatcaaccg
     2401 cgcgagttcg gcgtccagga tttctgcatg aactgcaatg tctgccagct caattgtccc
     2461 ggcgacgcga ttccgcagga tcacgtgatg acgcacggca ttagacgttg gctgattgat
     2521 ctggagaaat gttatcccta ttcgcgcctg cgcgatgaat attgtcatct atgcgtcgat
     2581 gtctgtccgt acagcgtaaa gtcgcatccc gaaacttata aagcgttcat gaaagagcgc
     2641 aagaaggtcg gttacaaaac gccgaaaacg tactgagccg ttatggaaaa actttacatc
     2701 aacgagatcg gtcccgatgg cgaggtcagc accgtgggct tattttactc gcagggcgag
     2761 gcgctaaaga ttatcgagat gct
//
LOCUS       DASULZ010000034         4219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_612612, whole genome shotgun sequence.
ACCESSION   DASULZ010000034 DASULZ010000000
VERSION     DASULZ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612612"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..855)
                     /locus_tag="VFY96_00670"
     CDS             complement(121..855)
                     /locus_tag="VFY96_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX5604994.1"
                     /translation="MKKTPKTLVGLLVLLLPRLPAALQAYDQIAVTDGATIRGSVKLE
                     GAAPKLAPLQITKYKEVCNDVPNESLMIGPNRGVRYAVITLEGIGKGKAVEREVVHEL
                     DNVKCRFAPHVQAASVGHFILLKNSDPILHTAHARFTNDQPQFNIGLYPGRVSRKPLI
                     APGLVKIQCEVHPWMSAYIIVTDHPYVAVTDLYGEYELSEVPPGSYQLKIWHESLGVQ
                     EKRIEIKAGGAQVVDFSFTPAAGVKK"
     gene            complement(902..1423)
                     /locus_tag="VFY96_00675"
     CDS             complement(902..1423)
                     /locus_tag="VFY96_00675"
                     /inference="COORDINATES: protein motif:HMM:NF040826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HEX5604995.1"
                     /translation="MATTVKKYDTSYKKRTDTDRDTYLPKRSPEEMLQCLGCAAFYHR
                     RHWTLTKPTGFDFSVRVHLCYCPACRKIKDQRASGELDLASNGNETKDVLRILRNEEG
                     RAREKNPLGRIMRIEEVNSGWRVQTTTEKLAQRLGRAVRKARGGKLQYKWSHNNKFLR
                     VTWDRQQTGSQSS"
     gene            complement(1445..1768)
                     /locus_tag="VFY96_00680"
     CDS             complement(1445..1768)
                     /locus_tag="VFY96_00680"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEX5604996.1"
                     /translation="MENQKDRFGETMRLAERAKEDIFFAEKDRELLEKLRAQLRKVDR
                     VEALRCPKCPGNLESYTFHGISLDRCHECGGVWLDKGELQAIVRKISRGPVGEWLDKL
                     TARNE"
     gene            2018..2416
                     /locus_tag="VFY96_00685"
     CDS             2018..2416
                     /locus_tag="VFY96_00685"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5604997.1"
                     /translation="MHRVSWAISAILFIVPFGVSFAADVDGGKEMYLKYCASCHGITG
                     EGDGAVSRDLKVKVPDLTLLRRNNQGVYPTDRVLSSIDGRRDVRAHGDRTMPVWGEVF
                     RKEHEKESYTELTTLLQAKVIAEYVGSLQK"
     gene            complement(2503..2970)
                     /locus_tag="VFY96_00690"
     CDS             complement(2503..2970)
                     /locus_tag="VFY96_00690"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5604998.1"
                     /translation="MLRVKKILAPTDFSELSKLGIRYALDMARDTRAEVIIYHVIDLG
                     EEWRGMHSQLRTYHDMVEEGRQLLDKFISDNFPDRADSVEVRLLVEFGGSFTNIVDLA
                     EKEHVDVIVMSTHGRTGLDHMMVGSVTEKVIARASCPVLVLPGHRRRALSSAA"
     gene            3211..3687
                     /locus_tag="VFY96_00695"
     CDS             3211..3687
                     /locus_tag="VFY96_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5604999.1"
                     /translation="MVAPEGCQRLDLKGADMGKYNQMTVSDVMTRNPVSISPSETVGQ
                     ADELMVENKIRQLPVVKNRVLVGIITDRDVRSFLSGSLLSAAEERERALGTEVNEVMT
                     ADPVTLAPDDELQDAIELLLEQKIGGVPVVDEAEGLVGIVTYVDILRCFLTGLQEE"
     gene            3690..4112
                     /locus_tag="VFY96_00700"
     CDS             3690..4112
                     /locus_tag="VFY96_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5605000.1"
                     /translation="MKNSKEETVREIMMGSPVTLKPDDTADLASDIISLGRIRHVPIV
                     EEGKLVGLVSARDLMGAAANQIFGLKQKTKSALLKTVPIKQIMKKRLITVEPETDIKE
                     AAHLMADRKVGCLPVLKEGSLVGLVTTTDVLRHMSKSR"
BASE COUNT          963 a         1124 c         1104 g         1028 t
ORIGIN      
        1 ccggtttgcc gttgatcgtg atgatcccct tgatcacgcc cgtaccgtca gcccaagcgt
       61 ggggaacgct ggaaaaaaac gcgaaaagtc cgccgaggat acacgacagc aatgccgctt
      121 tcatttcttc acccccgctg ccggcgtgaa actgaagtcg acgacttgcg ccccacccgc
      181 tttgatctcg atccgtttct cctggacgcc caagctctcg tgccagattt ttagttggta
      241 gcttcccggt ggaacttcac tgagctcgta ctctccgtaa aggtccgtca cagcgacgta
      301 gggatgatcc gtcacgatga tgtaggccga catccacgga tgcacttcgc attgaatctt
      361 aaccaggccg ggtgcaatca acggcttgcg gcttactcgc cctgggtaga ggccaatgtt
      421 aaattgaggt tgatcgttcg taaatcgagc gtgcgccgtg tgaagaatgg gatcagagtt
      481 ttttagcagg ataaagtgcc caacgctggc tgcttgcacg tgcggtgcga aacggcactt
      541 cacgttgtcg agctcgtgga caacttccct ttcaaccgct ttccctttgc cgatcccttc
      601 cagagtaatg accgcgtaac gaacaccgcg attcgggccg atcatcaagc tttcgttggg
      661 cacgtcgttg caaacttctt tgtatttggt gatctggagc ggcgccaatt tgggcgctgc
      721 accctcgagt ttgacgctgc cgcgaatcgt cgctccgtct gtgaccgcga tttgatcgta
      781 agcttgcagt gccgcgggaa gcctcggaag taatagtacg agcaaaccta cgagagtctt
      841 tggagttttc ttcatggccc ttccattctg cgtacgattg cgaggtttct tcagtcgcga
      901 atcaactgga ttgacttccc gtttgttgtc tgtcccaggt cacgcgcagg aacttattgt
      961 tatgtgacca cttgtactgg agcttgccgc cgcgcgcttt gcgcacggcg cggcccaagc
     1021 gctgcgcgag cttttccgtg gtcgtttgga ccctccaacc ggagttcact tcttcaatcc
     1081 tcatgatgcg ccccaggggg ttcttctcgc gagcacgccc ttcttcgttg cgtaaaatgc
     1141 gtaagacatc ttttgtttcg ttgccattgc tggcaaggtc aagctcgccc gacgcacgct
     1201 ggtctttaat ctttcgacag gcgggacaat agcacaagtg tacgcgcaca gaaaaatcaa
     1261 acccggtcgg tttcgtcagc gtccaatggc gccgatgata gaaagcggcg catcctaggc
     1321 actggagcat ctcttctggc gagcgtttcg gcagataagt atcccgatcg gtatcagttc
     1381 tcttcttgta gctggtgtca tattttttga ctgtagttgc catcgaaact cctcgttcag
     1441 aaattcattc gtttctagcc gtcagtttat caagccactc acccacagga ccacggctga
     1501 tctttctgac aatcgcctgt aactcacctt tgtccagcca aacaccaccg cattcatggc
     1561 agcgatcgag gctgatccca tgaaacgtat aactctccag gttgcccggg cacttcggac
     1621 agcgcagcgc ttcaactcta tcaaccttac gcagctgcgc tcgcaatttc tccaacagct
     1681 cgcgatcctt ttcggcaaaa aaaatatcct ctttcgctct ttcggctaga cgcatggttt
     1741 cgccgaaccg gtccttttga ttttccataa cgttcgcctc caggaattct catccgtttc
     1801 aaaacgcagc tttgatgcca gtgtaatgtt tcgttgcgtg cccgctgttt tatgaattgg
     1861 aggcgcgctc gtgcgctagt tattcatttt gttgtgcagg gaaatttgcc aaaattgcaa
     1921 acacaggcac ccggtttcgc atcattagga aatgtgctaa ttggcaactg attcgttccg
     1981 gggatcgcaa gcgatatcgc aaagggatgt taactttatg catcgcgtct cgtgggcaat
     2041 ttcagcaatc ctattcatag tgccgtttgg ggtctccttt gctgccgatg tcgatggcgg
     2101 caaagagatg tacttaaaat attgtgcttc ctgtcacggc atcacaggcg aaggagacgg
     2161 tgcggtcagt cgtgatttga aagtcaaagt gccggatctg acgctgttaa gacgcaacaa
     2221 ccaaggcgtg tacccgactg accgagtgct gtcgtcaatc gacggccggc gcgacgttcg
     2281 tgctcacggt gatcgcacta tgccggtatg gggggaagtt tttcgaaaag aacacgaaaa
     2341 agaaagttat accgagctca ctacgcttct ccaagccaag gtaattgccg aatacgtcgg
     2401 cagtttgcag aaatagacgt tcattcagct atttggcagg ctgcttcgcc atctagctct
     2461 cacccgctgt cgacatcatg caggccgtcc tgcagcgagt gtttaagcgg cgctcgacag
     2521 agcccgtcta cgatgtcctg gcaacaccag tacggggcag gaagcgcgtg caatgacttt
     2581 ttccgtcacg ctacccacca tcatatgatc caatccagtg cggccgtggg tcgacataac
     2641 gatcacatca acgtgttctt tctcggccaa gtcgacaata ttggtaaaag acccgccgaa
     2701 ctcaacgagt agcctgactt cgaccgaatc ggcccggtcc ggaaaattat cgctaatgaa
     2761 tttatcgagg agctggcggc cttcttccac catatcgtgg taggttctca gttggctgtg
     2821 catgcctctc cattcttcgc cgagatcgat aacatggtag atgatgacct cggccctggt
     2881 atcgcgggcc atgtctagcg catagcgaat gcccagcttc gataattctg aaaaatccgt
     2941 cggcgccaat atttttttta ctctaagcat atgaccccct aatccaccgt gttgtaatcg
     3001 actctccgca agaaagatgc ccgctggaaa ccgcccagga atcgaactat ggcgttccgc
     3061 ggttaatact ctcgcatttt tcagaaaaaa tgacggcatt cttgtcaggc gcgcgaaaat
     3121 ttgttcgtct agtgcagcca agctgatgaa atagcggcga catctcgcat ggcgattggc
     3181 atatgctgtg ctgaattgac gagttgagtc gtggtcgcgc cagaaggctg ccagcggctc
     3241 gatctgaaag gagccgacat gggaaaatac aatcagatga cagtgagcga tgtgatgacg
     3301 agaaatccag tgagcatttc accgtcggaa accgtcggac aggctgacga attgatggtg
     3361 gagaacaaaa ttcggcaact accggtagtc aaaaatcgtg tccttgtggg catcatcacg
     3421 gaccgcgatg ttcgctcttt tctcagcggc tcattgctat ccgcggctga ggagcgcgaa
     3481 cgcgcgctcg gcaccgaggt gaacgaggtc atgaccgccg atcctgttac tctggcgccc
     3541 gacgacgaat tgcaggatgc tatagagctt ttgctcgaac aaaagatcgg tggcgtgccg
     3601 gtggtcgatg aagcggaagg tctggtcggc atcgttacct acgtcgatat tttacgatgt
     3661 ttcctgacgg ggctgcagga ggaataatca tgaaaaacag caaagaagaa actgtccgcg
     3721 agatcatgat gggttccccg gttacgctga agcccgatga taccgctgat ctcgccagcg
     3781 acatcatctc gcttggacga attcgtcacg ttccgatcgt cgaagagggc aagctcgtcg
     3841 gcctcgtaag tgcgcgcgat ttgatgggcg cggcggcgaa ccaaatcttc ggactcaagc
     3901 agaagactaa gtcagcgctg ctgaaaaccg taccgatcaa acagataatg aaaaaacgcc
     3961 ttatcaccgt cgagccggaa acggacatca aggaggcagc gcaccttatg gcggaccgta
     4021 aggtcggctg tttaccggta ttaaaggaag gatctctggt cgggctcgtc acgacgaccg
     4081 atgttctccg tcacatgagc aaatcacggt agagcgcgtc agcgatttct gcaacgagcg
     4141 aacgccgcac aacgataaaa ttattgagga ggtcgtatgg gaggttttct tggcgcaaaa
     4201 gaggttctga ccagagaac
//
LOCUS       DASULZ010000035         2346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_105330, whole genome shotgun sequence.
ACCESSION   DASULZ010000035 DASULZ010000000
VERSION     DASULZ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105330"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..823)
                     /locus_tag="VFY96_00705"
     CDS             complement(74..823)
                     /locus_tag="VFY96_00705"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX5605001.1"
                     /translation="MSVAEVKDPAEYVKEHYRKVAARWRARVTDSPFMQQLRAGTLPR
                     DALRTFFKNWGSYTIEINTVEAASYHKHIAFFRKHRDLMARMAEKLADELIHPQPPGH
                     VHVVIETAKALGIGEDEIFLAPMLAEFRAKLDFKRSLLWEGTMAEFYAAGATEEQTGY
                     WSAEFFQALTTHYGLTREQAIYFSTHEEADLKEHDGVMGHGSFRRLVLRTLIEDGMAM
                     ARPGYTLEYCGMTAVDLHGVCLQAALNAPRS"
     gene            complement(951..1841)
                     /locus_tag="VFY96_00710"
     CDS             complement(951..1841)
                     /locus_tag="VFY96_00710"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX5605002.1"
                     /translation="MIRGVLLPIITPFDEKVRVDEQMMRALIDFHIKAGVQGLFVLGS
                     TGQGPAMTIEERKEAAAIALDQAKSRVPAIIHVGTADAQTTVELAEHAAAHKADAVAI
                     VPPYYYSDHTEFEIIAHYKAVAKAVPLPIFIYENPKYSGISIPPGLAVRMKEQVPMLK
                     GIKVAYGQGALLEYARLLPDVSVFTGNADLFGLVPFGVAGMINPPTSFVPELCVELFG
                     ALDRQDYPRAVKAQKRVDTATRLVAARLRKYGRVPLQEVFRMRGFAVKRFPKWETEQM
                     PKDEIAKLERELRSEGILGQ"
     gene            complement(1887..>2346)
                     /locus_tag="VFY96_00715"
     CDS             complement(1887..>2346)
                     /locus_tag="VFY96_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605003.1"
                     /translation="VHAKMDAAALMTEGSRCEHWMFVTPNDDEHFMLFTANNYLGPME
                     NFFDHLKELRANETPLQEIKPYDKRKYMPYKGNIRQEDIMTQGTQGLLGERSEQLGSS
                     DRGIIKFRKIVLDAINTSLGGGRPKGVIADKDDDGIFRLDTQVGLRPKVT"
BASE COUNT          482 a          681 c          631 g          552 t
ORIGIN      
        1 ctttgagttc tgcgctattt ttgcggcgcg gcgggacggt tggactttgg cgcgttctct
       61 gcattgaaga gaattagctg cgcggcgcgt tgagggccgc ctgcagacaa actccatgca
      121 ggtcgaccgc ggtcatgccg cagtattcga gcgtgtagcc gggacgcgcc atcgccatac
      181 cgtcttcgat gagcgtacgc agcaccagac gccggaagct tccgtgtccc atcacgccgt
      241 catgctcttt gagatcagct tcttcatgcg tcgaaaaata gatcgcctgc tcgcgtgtca
      301 atccatagtg ggtcgtgagc gcctggaaaa actccgcgga ccaatagccg gtttgctctt
      361 cggtcgcgcc ggcggcataa aactccgcca tggtgccttc ccacaacaac gaacgcttga
      421 agtcgagctt cgcacgaaac tcggcaagca tcggcgccag gaaaatttca tcctcaccga
      481 tgcccagcgc cttggcggtt tcgatcacga catgaacatg tcccggcggt tggggatgga
      541 tcaattcatc ggcgagcttt tccgccatgc gcgccatcag atcgcgatgc ttgcgaaaaa
      601 aggcgatgtg cttgtggtac gacgccgctt cgacggtatt gatttcaatc gtataggagc
      661 cccagttttt gaaaaaagtc cgcagcgcgt cacgcggcag tgtgccggct ctcagttgtt
      721 gcatgaacgg agaatccgtg acccgcgcgc gccagcgcgc cgccactttc cggtaatgct
      781 ccttcacata ctcggctggg tctttgactt cggcgaccga catgttcgtt cctttttctt
      841 ttccgcccga ggggaatcgt gcccctagcg tattcgagct ggcgtttgaa cgatgctctg
      901 gcagcaatca ggcgagatac agagcgctag caaaatcatc cgagcgattt ttattgcccg
      961 agaatgccct cgcttctgag ctcgcgctcc agttttgcaa tctcatcttt gggcatttgc
     1021 tcagtttccc atttcggaaa acgcttcacc gcaaagccgc gcatacgaaa aacttcctgc
     1081 aacggcaccc ggccatattt ccgcaaacgc gcggccacca gccgcgtcgc cgtatcgacg
     1141 cgcttctgcg cttttacggc gcgcggataa tcctggcgat ccaaggcgcc gaaaagctct
     1201 acgcaaagct cgggaacgaa gctcgttggc ggattgatca tgcccgcgac gccgaacggc
     1261 accaaaccga acagatccgc attgccggtg aagaccgaaa catctggcag tagtcgagca
     1321 tattccagca atgcgccttg cccgtaggcg actttgattc ccttgagcat cggcacctgc
     1381 tctttcatac ggaccgccag tcccggcgga atcgagatgc cggaatattt cgggttctcg
     1441 tatatgaaga tcggcaatgg tacggctttg gcgacagctt tataatgggc gatgatctcg
     1501 aattcggtgt gatcggagta atagtaaggc ggcacgatcg ccacggcgtc ggctttatgc
     1561 gccgcagcgt gctcggccaa ttccaccgtg gtttgcgcgt ccgcggtacc gacatggatg
     1621 atcgcaggaa cccgcgactt cgcctgatcg agggcaattg ctgccgcttc tttacgctct
     1681 tcgatggtca tcgccggccc ttgccccgtg gatccgagca caaacaaacc ctgtacgccg
     1741 gctttgatgt gaaaatcgat cagcgcccgc atcatctgct cgtcgacgcg gaccttctca
     1801 tcaaacggcg taatgatcgg cagcaacact cctcgaatca ttagcgttcc cctttcaatt
     1861 ctttgcgtca gacgaaaaat ctggccttac gtgacctttg gccgcaagcc gacttgcgtg
     1921 tccaaacgaa aaatgccgtc gtcatctttg tccgcgataa cgcctttggg gcggccgccg
     1981 cccaatgagg tattgatggc gtccagaacg attttacgaa atttgatgat gccgcgatcc
     2041 gatgagccaa gttgctcgga tcgttcgccg agcaggccct gcgttccctg agtcatgata
     2101 tcttcctgcc gaatattgcc cttgtaaggc atgtattttc gtttgtcgta cggctttatt
     2161 tcctgtaggg gagtctcgtt ggcgcgcagc tcttttaggt ggtcgaagaa attttccatg
     2221 ggtcccaagt aattattcgc cgtgaaaagc atgaaatgct catcatcatt gggggtcaca
     2281 aacatccagt gttcgcaacg cgagccttcg gtcatcaacg cggcggcatc cattttggcg
     2341 tgaaca
//
LOCUS       DASULZ010000036         3301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_917214, whole genome shotgun sequence.
ACCESSION   DASULZ010000036 DASULZ010000000
VERSION     DASULZ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917214"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..1236)
                     /locus_tag="VFY96_00720"
     CDS             complement(151..1236)
                     /locus_tag="VFY96_00720"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5605004.1"
                     /translation="MMKAFVHFLIILAALFSQMNDPAHAASPREPEEIWQELLKLPAD
                     ERQKRLVAGARAEGKAIVYGNISADHLEKLRIDFDKRYGVKLDGYRAAGERVANRLLT
                     EARSGKLDADVMAPSNEHIPTLIKAGIAGRYNSPERAAFPDSHKDRLGYWTAYDYNVA
                     VIAYNSRLVPAQEAPKKYEDFLDPKWKGNFAIDMDPDKSIMGWFKIWGVERAKKYLQA
                     ISKNEVVVRKGHTLLTQLLCAGEFKAAIDLYAYRLADLKHTRGCPVEISYPDPTPATP
                     SPVGVIKKTPRPYAAALLLDYLLSEPAQRIFAEAGRISARRDVRPRYPEIDLEAKGVK
                     ILLLTPDDAVQYEKPYQQLREEFLLNR"
     gene            complement(1248..2693)
                     /locus_tag="VFY96_00725"
     CDS             complement(1248..2693)
                     /locus_tag="VFY96_00725"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX5605005.1"
                     /translation="MAKSLRTFLADMRRSCPTDVVSISRTVNPLNYDITAIVKHLGAR
                     KKFPVLVFEQPLNAHGRASEMKLVISTENSQKKIQVALGLPSAMNRAEMARECLRREA
                     AKIGPVLVRKEAAPVKEIVQTGNQIDLYELPLMRHHEMDGGPYIDMSSVARDRSSGVY
                     NCSYHRMEVKDRNHTGFLMTLQHMWRIFRAYEDAGEECPVATVIGHHPAYQMGACYGG
                     AFDVSEYEIIGGYLQEPLRLTPSETWGERLLVPADAEIVIEGALLPGQRMVEGPFGEV
                     NGYLGVQGFQQSAHYEVRAITRRRGAMHTSILTPEGDKPWMDLAREGAYLRRCREAVP
                     GVQAVCASGRHALLNVFISMKKMSEGDPGRAAAAVLTWDWAKNVFVFDEDIDVYNPTE
                     ILWGLATRVQPHRQISIVPEIMRGSLIDPSMIDARKTSVMIIDATKPLDRPFSPVSKC
                     PDEALERIKLEEFVPSEVLQHIPVDRTTYWA"
     gene            2990..>3301
                     /locus_tag="VFY96_00730"
     CDS             2990..>3301
                     /locus_tag="VFY96_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605006.1"
                     /translation="MATQRYNAGIQRAKGLLEIAAGLYDSSLSYQTRRAAENVLQTDT
                     DLGRVLRLSLGTKKSETRPEVTLSFATGSFREIIAVGQGKYSLAWVNPSVLLTMAYRG
                     TG"
BASE COUNT          683 a          996 c          879 g          743 t
ORIGIN      
        1 cgagcaaaca gagcacccgg ccgtcatttt cagtgatctc gacaaaagaa ttcgtcccga
       61 acaacacttg gactcgctgg tagccggttt cgcgatttcc ctcgacaatg actgctctga
      121 ttttcgccat cgacagcctc gttcattagc ttaacggttc aagagaaact cttctcgcaa
      181 ttgctgatag ggtttttcgt actgcaccgc gtcatccgga gtaaggagga ggatcttcac
      241 tcccttggct tcgagatcga tctcaggata gcgcgggcgc acgtcgcggc gcgcagaaat
      301 gcgccccgcc tcggcgaaga tgcgctgtgc cggttccgag agaagatagt cgagcaacaa
      361 cgcggcggca tagggccgtg gggttttttt aatgaccccc accgggctcg gagtggcagg
      421 cgtcggatcg ggataggaaa tctccaccgg gcaaccccgt gtatgcttca ggtccgccag
      481 gcggtaagcg taaagatcga tggccgcttt gaactctccc gcgcagagca attgggtgag
      541 caaggtatgc cctttgcgca ccaccacttc atttttgctg atcgcctgca gatatttttt
      601 cgcccgctcg actccccaga ttttgaacca gcccatgatc gatttgtcgg gatccatgtc
      661 gatggcgaaa ttgcccttcc atttgggatc gagaaagtct tcgtactttt ttggcgcttc
      721 ctgcgctggc accagccggc tgttatacgc aatcaccgcc acgttgtaat cataggcggt
      781 ccagtagccg agccggtcct tgtgcgaatc gggaaacgcc gctcgctccg gtgaattgta
      841 ccgcccggca atacccgcct tgatcagggt gggaatatgc tcgttgctcg gcgccatcac
      901 gtcggcatcg agcttgccgc ttctcgcctc ggtcaagagc cgattcgcga ctctttcacc
      961 tgccgcgcgg tagccatcga gtttgacccc atagcgttta tcgaaatcga tgcgcagctt
     1021 ttcgagatga tcggcgctga tattaccata aacaatcgcc tttccttccg cccgcgcacc
     1081 tgcgacaagc cgtttttgcc gctcgtcggc tgggagcttg agcaactcct gccagatctc
     1141 ttcgggctcg cgcggcgaag cggcgtgcgc cgggtcattc atctgggaaa aaagcgcggc
     1201 caagattatt aagaagtgca cgaaggcttt catcatttta cctccgatca ggcccagtat
     1261 gtcgttcgat cgaccgggat atgctgcaga acttcactag gcacgaactc ctccagcttg
     1321 attctctcga gcgcctcgtc gggacatttt gaaaccggcg agaacggacg atcgagaggt
     1381 ttggtggcat cgatgatcat caccgatgtt tttcgggcat cgatcatgga tggatcgatc
     1441 aagctgccgc gcatgatctc gggaacgatg gaaatttgcc gatgcggctg cacgcgcgtg
     1501 gccagacccc agaggatctc cgttggattg taaacatcga tgtcttcatc gaagacgaag
     1561 acgttctttg cccaatccca ggtcaaaacc gctgccgcgg cgcggccggg atcgccctcg
     1621 gacatttttt tcatcgagat aaagacgttg agcaacgcgt gccggccgct cgcgcagacc
     1681 gcctggacac cgggcacggc ttcccgacag cgccgcagat aagccccttc gcgcgcgagg
     1741 tccatccagg gcttgtcgcc ttctggagtc aaaatcgagg tatgcattgc gccgcgccga
     1801 cgtgtaatcg cgcgcacttc gtaatgcgcc gattgctgaa acccttgcac gcctaaatag
     1861 ccgttcactt cgccgaacgg tccctcgacc atgcgctggc cgggcaagag cgcgccctcg
     1921 atcacgatct cggcgtcggc tggaaccagc aaccgctcgc cccaggtttc ggagggcgtg
     1981 agccgcagcg gctcctgcaa atagccgccg atgatttcgt attcgctgac gtcgaacgct
     2041 ccgccgtaac aagcgcccat ttgataggcc ggatgatggc cgatcaccgt ggccaccgga
     2101 cattcttcgc cagcgtcttc gtacgcgcgg aaaatccgcc acatgtgctg caaggtcatg
     2161 agaaacccgg tatggttgcg gtctttgact tccatccgat gatacgagca gttgtaaacg
     2221 ccactgcttc tgtccctcgc cacactcgac atatcgatgt aaggtccgcc gtccatctca
     2281 tgatggcgca tcagtggaag ctcgtagaga tcgatctgat tgccggtctg gacgatttct
     2341 ttgaccggcg cggcttcttt gcgcaccagg accggcccga tcttggccgc ttcgcgccgc
     2401 aagcactcgc gcgccatctc ggcccggttc atggcgctcg gtaacccgag cgccacctga
     2461 atttttttct gcgagttctc cgtgctgatc accagtttca tttcgctagc gcggccgtga
     2521 gcgttgagcg gctgctcgaa aaccagcacc ggaaatttct tgcgagcgcc gagatgcttg
     2581 acgattgccg taatgtcgta attgagcgga ttgaccgttc tcgagatcga gactacgtcg
     2641 gtcggacagg agcgacgcat atccgctaga aatgttcgta gagattttgc catcgatctg
     2701 gttcaattag gacgattcac ccaatcgcgc gaggcctgcc ccaaccccaa ccaccacgat
     2761 cccaagcgcc gccttgcgcc atcacgagct tcattatttt aataacatat cccgcaggag
     2821 cgcttcatcg ttcactccta cctcgtacca cgcaggaaat gacgtaaaca acaccattta
     2881 ccagatccta ttaggatcac ccatgctact acactcctcg cacaaaatgc ttgtcacaac
     2941 cgtaccgggt gattattgtg gggccactca caggaggcat ccactggcaa tggctacgca
     3001 acgttataat gccggcattc agcgcgccaa gggtctgctc gagatcgcgg ctgggcttta
     3061 cgacagttcg ttatcctatc aaacccgacg tgctgccgag aacgtgctgc aaaccgatac
     3121 tgatctggga cgagtgctga gactttccct cggcacaaaa aaatccgaaa cccgtcctga
     3181 agtaacccta tcgtttgcca ccggcagctt tcgcgagatc attgccgtcg gccaaggaaa
     3241 atattccctc gcctgggtca atccttcggt gctcctgacc atggcttatc gcggcacagg
     3301 t
//
LOCUS       DASULZ010000037         3493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_815984, whole genome shotgun sequence.
ACCESSION   DASULZ010000037 DASULZ010000000
VERSION     DASULZ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815984"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1382)
                     /locus_tag="VFY96_00735"
     CDS             complement(<1..1382)
                     /locus_tag="VFY96_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX5605007.1"
                     /translation="MTATALQRRHALRNIAIIAHVDHGKTTLVDGMLRQTGIFRANEQ
                     VAERVMDSMDLERERGITIMAKNTTIHYGDVKINIVDTPGHADFGGEVERTLAMVDGV
                     MLLVDASEGPLPQTRFVLKKALEAKLPPIVCINKIDRSDARVAAVLDEIYDLFIDLDA
                     TEEQLDFPVVYTNARAGTATRDLKQPGENLKPLFDLIVDTLPGPAFDPTGSTQFQANN
                     LDYNDYVGRLAIGRLKNGALAIGNYTLCRSDGAQQQVKVTQVFGWRGLKRVEIEAAEA
                     GDIVAVAGIEDIGIGDTIADRENPKPLPALRIDEPTIAMVFSANNSPWAGREGDFVTS
                     RKLRERLFLEQKKNVSLRITDMEQPDSFQVTGRGEFQLAIIIETMRREGYELMVSKPT
                     VVTREIDSKLHEPLELLLIDIPEDFIGVVSQLLAMRKGKMTKMIHAGSSRVRLEFSVP
                     SRGLIGFRSR"
     gene            1497..3236
                     /locus_tag="VFY96_00740"
     CDS             1497..3236
                     /locus_tag="VFY96_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5605008.1"
                     /translation="MGRISQYIWRYRRRYLFGGLCLIGTATLVMWIPWWIREAIRIIE
                     HGGALSDVRYYVLLIIAAAIAQGFVRTYSRALIFNAGRNVEYDLRNDLFAHLEKLPLS
                     FYHSQRTGDLMSRVINDISAVRTMLGPGVLNFANAPVYYVYAVALMLSMDVRMTLAAL
                     APFPLLIYVARRFRGRIMKSSLEVQQQMSALSSHVQENLSGMHVVKAYAQEEFQTQHF
                     IGLNKDYEVKSMEMAKMRGIVNPVMQGINGLTVLIVIWYGGVRVMRGDLLVADIVAFI
                     AYLNVLAWPTAAFGWMLSLVERGRAAMRRLEEILEVKPEITDPPSPVAIDALKDGIEF
                     RDVSFAYERQRNGHTALSAINFTLPVGRSVGLVGRVGSGKSTLAQLVARLIDASSGGI
                     FLDGQDIRKVALRDLRRLIGYVPQEPFLFSTSLKANLALGKEQCSSEELARATAIAKL
                     DRDLEIFPLGLDTVVGERGVTLSGGQKQRATLARALVMDPPVLILDDCLSSVDAQTEA
                     EILHGLCAILKEKTCLIISHRISAVREADEILVLDEGAIIERGNHDELIRRGGVYAEL
                     CQQQRLSEELEQI"
     gene            3237..>3493
                     /locus_tag="VFY96_00745"
     CDS             3237..>3493
                     /locus_tag="VFY96_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5605009.1"
                     /translation="MTEAIEQEEVLGKAYDLRLIRRLWQFILPYKRLFLISLLLLPLQ
                     QAFGLAQPYLMKIGIDQYIAGKDLWGLQSVMLLFLGALIGET"
BASE COUNT          689 a          984 c         1033 g          787 t
ORIGIN      
        1 aaacgcgagc gaaaaccgat caggccgcgc gaaggcacgc tgaactccag tcggacacgg
       61 ctcgaaccgg catgaatcat tttcgtcatc ttgcctttgc gcatggccaa caactgcgac
      121 accacgccga tgaaatcctc cggaatgtcg atgagcaaca attccagagg ctcgtggagc
      181 ttgctatcga tttcacgcgt gaccacggtc ggcttggaga ccattagctc atacccttca
      241 cggcgcatgg tctcgataat gatggcgagc tgaaattcac cgcgcccggt aacttggaaa
      301 gagtcgggtt gctccatgtc ggtgatgcgc aggctgacgt ttttcttttg ttcgagaaaa
      361 agccgctcgc gaagcttgcg tgacgtgaca aaatcgccct cgcgtcctgc ccagggcgaa
      421 ttattcgcgc tgaaaaccat cgcgatcgtc ggttcatcga tacgtagtgc cggcaggggc
      481 ttaggatttt cccggtcggc gatggtatcg ccgatgccga tatcttcgat gccggcgacg
      541 gcgacaatgt cgcccgcctc ggcggcctcg atctcgaccc gcttcaagcc tcgccagcca
      601 aagacctgtg ttacttttac ctgctgttgc gcgccatcgc tgcgacacaa agtgtaattg
      661 ccaatggcaa gcgcgccgtt tttcaaccgt ccgatggcga gccggccgac gtaatcgttg
      721 tagtcgagat tgttggcttg aaattgcgtc gagcccgtcg gatcgaaggc gggcccggga
      781 agcgtatcca cgatcaaatc gaacagcggc ttcaaatttt ctcccggttg cttcaagtcg
      841 cgggtcgcgg tgccggcgcg agcgttggtg taaaccacgg gaaaatcgag ttgctcctcg
      901 gtggcatcga ggtcgatgaa aagatcatag atttcgtcca acacagcggc gacgcgggcg
      961 tcggatcggt cgattttgtt gatgcagacg atcggcggca acttggcctc gagggctttt
     1021 ttcagaacaa accgtgtttg cggcagcggc ccctccgaag catcgacgag caacatcacg
     1081 ccatcgacca ttgcgagggt gcgctcgacc tcgccgccga agtcggcgtg ccccggcgta
     1141 tcgacgatgt tgatcttgac atcgccgtag tgaatcgtgg tgttcttcgc catgatcgtg
     1201 atgccgcgtt cgcgttcgag atccattgaa tccatgacac gctcggcaac ctgctcgtta
     1261 gcgcgaaaaa ttccggtttg gcgcagcatg ccatcgacca aagtggtttt gccgtgatcg
     1321 acgtgcgcga taatagcgat gttgcgcagc gcgtggcgcc gttggagcgc ggtggcggtc
     1381 atcactcatg gttattaggg agcagtctcg cattgtcaag cgaccttgtt gggtgcggca
     1441 cggcggcaac catttgttgc gaccgccgag gccgtagagt attctaatac ctctacatgg
     1501 ggcgcatttc ccaatacatc tggcgttaca ggcgacgtta tctcttcggc ggcctgtgtc
     1561 ttatcggcac ggcgaccttg gtcatgtgga ttccctggtg gatccgcgag gcgatccgca
     1621 tcatcgagca cggcggcgcg ctcagcgacg tgcgatatta cgtgttgttg atcatcgccg
     1681 ccgcgattgc gcaggggttt gtccgaacct actcgcgcgc gctgattttc aacgccgggc
     1741 gtaacgtcga gtacgattta cgcaacgatc tattcgccca tttggaaaaa ctgccgctgt
     1801 cgttttatca ctcgcaaagg accggcgatc tgatgtcgcg cgtcatcaac gatatctccg
     1861 cggtccggac gatgctcggt ccgggcgtgc tcaatttcgc caacgcgccg gtctactatg
     1921 tctatgcggt cgctttgatg ctctcgatgg atgtgcgcat gaccctcgcc gctttggcgc
     1981 cgtttccgtt gttgatctac gtagcccgcc gctttcgcgg ccggatcatg aaaagctcgc
     2041 tggaagtgca gcaacaaatg tcggctttga gcagccacgt gcaggaaaac ttgagcggca
     2101 tgcacgtcgt caaagcttac gcgcaggaag agtttcaaac tcagcacttc atcggcttga
     2161 acaaagatta cgaagtcaaa agcatggaaa tggccaagat gcgcggcatc gtgaatccgg
     2221 tgatgcaggg catcaacggc cttactgtgt tgatcgtgat ttggtacggc ggcgttcgcg
     2281 tcatgcgcgg cgatcttttg gtggccgaca tcgtcgcctt tatcgcctat ctcaatgtgc
     2341 tcgcctggcc gacggcggct ttcggttgga tgctgtctct ggtcgaacgg ggccgcgctg
     2401 ccatgcgccg gctcgaagag attctcgagg tcaagcccga gatcaccgac ccgccatcgc
     2461 ccgtcgcaat cgatgcgctc aaagacggta tcgagtttcg cgatgtttcc ttcgcttacg
     2521 agcggcaaag gaacggccat accgcgctat ccgcgatcaa tttcacactg ccggtaggac
     2581 gttcggtagg cttggtgggc cgagtcggtt ccggcaagag cacgctggcg cagctcgtgg
     2641 cccggcttat tgacgcttcg agcgggggaa ttttcctgga cggacaggac atccgcaagg
     2701 tcgcgctgcg tgatctgcgc cggttgatcg gctacgtccc ccaggagccg tttctatttt
     2761 cgacctcgct caaggccaat cttgccttgg gcaaggaaca gtgttcatcc gaagagctgg
     2821 cgcgggctac cgcgatcgcg aaattggatc gcgatctgga aatttttccg ctcggcttag
     2881 acacggtcgt gggtgagcgc ggcgtgacgc tttccggggg gcaaaaacag cgagcgactc
     2941 tggcccgcgc gctggtgatg gatccgccgg tgctgattct cgacgattgc ctctccagtg
     3001 tcgatgccca gaccgaagcc gagattctcc acgggctctg tgcgattctg aaagaaaaaa
     3061 cctgcctgat catctcgcat cgaatttctg cggtcagaga agccgacgag atcctggtcc
     3121 tcgacgaagg agcgatcatc gagcgcggca accacgacga actgatccgg cgcggcggcg
     3181 tctatgcgga gctttgccag cagcagcgtc tgagcgagga actggaacag atctagatga
     3241 ccgaagcgat cgaacaagaa gaagtcctcg gcaaagccta cgatctcagg ctgatcagac
     3301 ggctttggca gttcatcctt ccctacaagc gtctgttcct tatcagccta ctgcttttgc
     3361 ctttgcagca ggcttttggt ttagcccagc cgtacttgat gaagatcggc atcgatcaat
     3421 acattgccgg taaggatctt tgggggctgc agagtgtgat gctgctcttc ctgggcgcgc
     3481 tgatcggcga gac
//
LOCUS       DASULZ010000038         4009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_105687, whole genome shotgun sequence.
ACCESSION   DASULZ010000038 DASULZ010000000
VERSION     DASULZ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105687"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VFY96_00750"
     CDS             complement(<1..818)
                     /locus_tag="VFY96_00750"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5605010.1"
                     /translation="MAKRETSYELWLRQEGIPVIGGFGVEDVTELPLKSWKRTGGKGA
                     YIDLKGMEGFTGMYVSEIPPGGALHPENHLYEELIYIIAGYGATEIWSAGDNVRKMHF
                     EWQQGSLFAIPLNTRHRMINGSREPVFFLAVTSAPLMIDLLHNVPFVMGSDYTFADRF
                     DAESDYFVPTKTRKEVGGFINWETNFIADARAALVDPSEAKGFGVKITSFDMGGSTLV
                     GHIAEWPVGRYHKAHFHQGGAILLILRSRGYTLMWPPEAGIRPYQSGHGDQVVKV"
     gene            complement(821..2404)
                     /locus_tag="VFY96_00755"
     CDS             complement(821..2404)
                     /locus_tag="VFY96_00755"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX5605011.1"
                     /translation="MPQAYYEDFRGYLSELQNHGKLHRWNRPINKDTELMPLMRLQYR
                     GVADENRQCFLYENVVDSRGRRYDLRVATGMYGSSRQIAALGLGCAEPSEIYEKWRQA
                     LAKPHPTRRVSAGAVQEIVYTGAALKNFGVTSLPAPVEEPGFSGGIRATGPFITMDRE
                     TGVRNVGMYSGHFRAEDRLIAGIAPTHHAILYHHRSAISRQETLPVAIVLGALPDINF
                     VSAANLPYGVDELSIAGGIRERPVDVVPCKTIDLEVPAEAEIAIEGEMSFDRRERGEP
                     FSDYPGYLMVEREFRVVIDVKAITMRRNAIFTAILVGLPPSESNGISRTCREMMLYNH
                     LRYGCAMPEVLEVSCPEMGGGWNWWVIRMRKSHPSKPKQALHAAAGMDPTSKMIIVVD
                     EDIDPKDPDMVMWAMSFAMQPHQDAEIITGRVPLLDPSAHSLMRGREERSFPGAVGCS
                     GLLIDATRKGPFPPVGLPKKSYMEKALQIWQQAEMPKLNLKTPWYGYPLGLWDEEDDR
                     LAEKVAQGDYFQSKQVKGH"
     gene            3399..3743
                     /locus_tag="VFY96_00760"
     CDS             3399..3743
                     /locus_tag="VFY96_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5605012.1"
                     /translation="MYQLQKRGLKFGVIPEFKDYGAYLTPGFGSAMFVNRAPHPNAGK
                     VFLNWLLGKEGQTAFSKGMGYVSRRLDVPTDHVPSYWVPKTGVKYWPGYYEEDAKMSS
                     EQEGFLKTLFGR"
     gene            3771..>4009
                     /locus_tag="VFY96_00765"
     CDS             3771..>4009
                     /locus_tag="VFY96_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009182750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605013.1"
                     /translation="MPRTLRPSKKRALRPNFTDRAVHTLPSNQQRTGILFGALGAVIV
                     RVTLTFAAAQLLTFPAVQLIGGLLLIWIAVKLLTQ"
BASE COUNT          897 a         1145 c         1055 g          912 t
ORIGIN      
        1 actttcacga cttgatcgcc atgaccggac tgataaggcc gaatccccgc ttcaggcggc
       61 cacatgagcg tgtagcccct ggagcggaga atcagcaaaa tcgcgccgcc ttgatgaaag
      121 tgcgccttgt gatagcgccc caccggccac tcggcaatat gtcctacgag cgtgctgccg
      181 cccatatcga acgaagtgat cttgacgcca aaacctttgg cttccgaagg gtcgaccagg
      241 gccgctcgcg catcggcgat gaagttggtt tcccaattga tgaacccgcc aacttcttta
      301 cgcgtcttcg tcggcacgaa ataatcgctc tcagcatcga agcggtccgc gaacgtgtag
      361 tcgctgccca ttacgaatgg aacgttgtgg agcagatcga tcatcaacgg cgcgctggta
      421 accgcgagaa aaaaaaccgg ttcgcgactg ccgttgatca tccgatggcg tgtgttgagc
      481 gggatcgcaa agaggcttcc ctgttgccat tcaaagtgca tcttgcggac gttatcgccc
      541 gccgaccaga tttccgtcgc gccatatccg gcaatgatat agatcagctc ttcgtacaag
      601 tgattttcgg gatggagcgc gccgcctggt ggaatctcgc tgacatacat tccggtgaag
      661 ccctccatac ccttcagatc gatgtaggct cccttgcctc cggtgcgttt ccacgacttg
      721 agcggcaact cggtcacgtc ttcaacaccg aatccaccaa tgacaggaat tccctcttgc
      781 cgcagccaca actcgtaact ggtttcgcgc ttcgccacga tcaatgtcct ttcacttgtt
      841 tcgattgaaa ataatctcct tgggcgactt tttccgcgag gcgatcgtct tcttcgtccc
      901 agagaccgag cggatatcca taccacggcg tcttcagatt gagcttcggc atctcggctt
      961 gctgccaaat ctgaagagcc ttctccatgt aagacttctt tggcaatccg accggcggaa
     1021 atggcccttt gcgcgtcgca tcgatcaaga gtccagaaca gccgaccgcg ccaggaaagc
     1081 tccgctcttc gcgaccgcgc atgagcgaat gcgctgaggg atcgagcagc gggactctgc
     1141 cggtaatgat ttcagcatcc tgatgtggtt gcatcgcaaa gctcatcgcc cacatcacca
     1201 tgtccggatc ctttggatcg atgtcctcgt cgacaacaat gatcattttg ctcgtcggat
     1261 ccataccggc agcggcatgc agagcttgct tcggcttgct cgggtgactt ttacgcatac
     1321 gaatcaccca ccagttccag ccgccaccca tttctgggca ggaaacctcc aagacttccg
     1381 gcatggcgca gccgtaacgg agatgattgt aaagcatcat ctcgcggcac gtgcgcgaga
     1441 ttccgttgga ctcgctcggc ggcaggccaa ctagaatagc ggtaaaaatg gcgttgcggc
     1501 gcatcgtgat cgctttcacg tcgatcacga cgcgaaactc acgctcgacc atcagataac
     1561 cgggataatc gctgaatggc tcgccgcgct cgcggcgatc gaatgacatt tcaccttcga
     1621 tcgcgatctc cgcttcggcc gggacttcca gatcgatggt tttgcacggg acgacatcca
     1681 ccggccgctc gcgaatgccg ccggcgatgg aaagttcgtc caccccgtag ggaaggttcg
     1741 cggcggaaac gaagttgata tccggcaagg cgccgagcac gatggcgacg ggaagcgtct
     1801 cctgccggct gatggcgctg cggtgatgat aaagaatcgc atggtgcgtt ggcgcgatgc
     1861 cggcaatcag ccgatcctcc gccctaaaat gaccactgta catgccgacg ttgcgcacgc
     1921 cggtctcgcg atccatggta atgaagggtc cggtcgctcg gatgccaccg ctaaagcccg
     1981 gctcttcgac tggcgctggc aaactggtga cgccaaaatt cttcagcgct gctcctgtgt
     2041 aaacaatctc ctgtaccgcc ccagccgaaa ctcttctcgt cgggtgcggc ttcgctagcg
     2101 cttgacgcca cttttcatag atttccgatg gctcggcgca gcctagcccg agcgcggcga
     2161 tctggcgcga agatccatac attccggtgg cgacccgcag atcatagcgc cgaccgcggc
     2221 tgtcaacgac attttcgtag agaaaacact gtcgattctc gtcggcgaca ccacggtact
     2281 gaagcctcat cagcggcatc agttcagtgt ccttgttgat ggggcgattc cagcgatgca
     2341 acttgccgtg gttttgcagc tcgcttaagt acccgcggaa atcctcatag taagcctgag
     2401 gcattcgggc cgattccttt catctatgta gtcttctgcc gaaacggcct gtgcgatggt
     2461 gacaccggct cgcgagtacg tgactttttt cttggattac ttctgcgttg gctgagatag
     2521 ccaaatcttt attatcaaat attcaacgtg tcaagtattt tcgccatgaa atctttcgat
     2581 cggcgacaga gatcgtggag cattagcaga aataggagaa aagaaggtgg gcagagaaaa
     2641 tggagcgggt gaggagggtc gaactcccga gcttatgctt ggcaaacacg aaaagcaccg
     2701 gggaaagcag aaaaaatgct tataaatcaa tctccgaatt ccctgccctt ccccagcttt
     2761 ccttcgctga aaaatttttc acgtcattgc ccgcgtagag ccccacaagg atgagcttga
     2821 tcctgacgcg ccattgttcg tcagctagcg gggattcgat atccgtggac atttacagac
     2881 cgttccagca agcggagacg gccgaaatag atttgcttta attcgcttca ggaaaaaatc
     2941 ttggttgaaa atgtacatca accgtcctcg tcttgattcg cgctaatacg tatatcggag
     3001 gcgactaata acattttttt taggcatcaa aataaattga agggtctcgg cgcattgccg
     3061 gaaattggtt ggcggaggca cctgcaccca aactgtcgcc gacagagagt gcacggagat
     3121 ttctttacac tggtgaacga tctcaaaaac ccgggcgcga gtgttggtgc ggaaaaacgg
     3181 cagtggcacg atggccacac caaacggtgt cgatcagctc cgactttctg agcacctcgt
     3241 cgattgctct ctaacaactg tatcctctgg agctccccga ttcgatccaa tgctgggcca
     3301 ggtggcggcg gtacgaatcc ctacgttgtg cgcctctacc ccagcgtaaa tccacggcat
     3361 cgccgtcgta tccgcttaac tttgggcccg gcacggtcat gtatcagctt cagaaacgag
     3421 gactcaaatt tggcgtcata cccgaattca aagactacgg cgcatatctc actcccggct
     3481 ttggcagcgc gatgttcgtc aatcgcgcgc cgcatcccaa tgccggaaag gtttttctca
     3541 actggcttct tggcaaagaa gggcaaacgg cttttagcaa aggcatgggt tatgtgagcc
     3601 gcagactcga tgtccccacc gatcatgttc cttcttactg ggtgccgaaa acaggggtga
     3661 agtattggcc cggctactac gaggaagacg ccaagatgtc ttctgagcag gaaggatttc
     3721 tgaagacatt attcggaagg tagtttgacg ataacttctc ggatcagccg ttgccgcgca
     3781 cactccggcc atcgaaaaag cgagcgctac ggcccaactt tactgaccga gcggtccata
     3841 cgcttccttc gaaccagcaa cgcactggaa ttctgtttgg cgcgttaggc gctgtgatcg
     3901 tacgcgtcac cctgacattc gcagccgcac aactcctgac ctttccggct gtgcagctaa
     3961 tcggcggtct cctgctgatc tggattgccg tgaagcttct tactcaaaa
//
LOCUS       DASULZ010000039         2230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_816311, whole genome shotgun sequence.
ACCESSION   DASULZ010000039 DASULZ010000000
VERSION     DASULZ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816311"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2230
                     /locus_tag="VFY96_00770"
     CDS             <1..>2230
                     /locus_tag="VFY96_00770"
                     /inference="COORDINATES: protein motif:HMM:NF014503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605014.1"
                     /translation="ELASQLPKSVQVNLVTHSRGGLIGDLLCLDPFSDELIEQFKSDL
                     PEPGDVAEEERERLKKEIANAHAEQRAKLRDLRALLQDRQFAIRRYVRVAAPARGTLL
                     ASGNFDVFLSALLTLIGRVPYLYGNPLYYAFKRVVLEIAKNRTNAKLVPGIEAMLPDA
                     PMARFLASAKPRASAQFAAIAGDTEGGGLLKRLGVLFTDYAFFTGVDNDLVVDTGSMY
                     AGIAREAHGRALFDQGPTTSHFHYFQNDATRAALRDWLTVENTEALDLFKSLPGEVTE
                     PSLSEETARVAAARRSRGGAAAEASLPIVIVLPGIMGSHLWRSTQDRVWFDFPDLVTG
                     GLQKLSWSATTGSDAGDGVMPEKLFDMSYGDVCLYLMDTHRVERFPYDWRLPLNILAS
                     ELEKFLRRLLDETKEPLRPIRLLAHSMGGLVVRALIHQHPQLWDELMGRAGARFIMLG
                     TPNQGAHSMVEALLGKSDTLRSLARVDLQHDLQTILNIIGEFRGALQLLPKTGFRDVG
                     ETPFDEYYSITRWSGLKAEMKDFWFGDRVAAVPSEAALSQGKWLWDRDGLATPALPER
                     HTEKTIYVHGTAPLTACGIQKIDGRWKMIGTNEGDGTVTWKSGAISGIGRRYYMPAVH
                     GDLAATPEYFAGIAELLQSGATNQLQDRAPEVRDPEAAKPKPYEAGPPRYAMPEEAAH
                     ALMGAPKRKRLKARPVSTLKVIVKAMDLRFVTQPIMVGHYEQDAISGAESIIDRELVE
                     HAL"
BASE COUNT          462 a          632 c          688 g          448 t
ORIGIN      
        1 gagctggcca gccaattacc caaaagcgtg caagtcaacc tggttaccca ttcgcgcggg
       61 ggactcatcg gcgatttatt gtgcttggat cctttcagcg atgaattaat cgagcagttc
      121 aagagtgatc tgcccgaacc gggtgatgtg gcggaagagg agcgtgaacg gctcaaaaaa
      181 gaaatcgcca acgcgcacgc tgaacagcgc gccaagttgc gcgacctccg ggcgctgttg
      241 caggaccggc aattcgccat acgccgttat gtacgggtcg cggcgccggc gcgcgggaca
      301 ttgctcgctt caggtaactt cgacgtgttc ctttccgctc tactgacctt gatcgggcgg
      361 gttccgtacc tctacggcaa cccgctctac tatgcattca agcgcgttgt gcttgaaatc
      421 gccaaaaatc gcaccaacgc caagctggtt ccgggcatcg aagcgatgct acccgatgcg
      481 ccgatggcgc gctttctggc gtcggccaag ccgcgggcca gcgcgcaatt cgccgccatt
      541 gccggcgata ccgagggcgg gggcctgctg aaacgcctcg gtgtcctgtt taccgactac
      601 gcttttttta ccggcgtcga caacgatctg gttgtcgata ccggctctat gtatgcgggg
      661 attgcgcgcg aagcgcatgg ccgcgcgctc tttgatcaag gaccgacgac cagccacttc
      721 cattattttc agaacgacgc tacccgagcc gcgctgcgtg attggctgac ggttgaaaac
      781 acagaggctt tggacttgtt caagtctttg cccggcgagg tgacggagcc aagcttgagt
      841 gaagagacgg cgcgcgtggc cgcagcgcgg cggagccgcg gtggtgcagc ggcggaggcg
      901 agcctgccga tcgttatcgt cctgcccggt atcatgggct cccatctttg gcgcagtacg
      961 caagaccgag tctggttcga ttttcccgat ctggtgaccg gaggtctaca aaagctttcc
     1021 tggagcgcga ccacgggcag cgatgcgggc gacggtgtta tgccggaaaa actgttcgac
     1081 atgtcatatg gagacgtttg cctatatctc atggacacgc acagggtcga gaggtttcct
     1141 tacgactggc gtctgccgct caatatactt gcgagtgaac tggagaaatt cttgcgccga
     1201 ctgttggacg aaaccaagga gccgttgcgg ccgattcggc tgctcgccca tagcatgggc
     1261 gggttagttg tccgtgcgct gattcatcag catccccaac tctgggatga actgatgggg
     1321 cgcgctggcg cgcgatttat catgctcggc acgccgaacc agggagccca ttcaatggtc
     1381 gaggcgctgc tcggtaaatc ggatacactc cgcagcttgg cgcgcgtcga tctccagcac
     1441 gacttgcaga cgattctcaa cattattggc gagtttcgcg gcgcgctgca gctcttgccg
     1501 aagaccggtt tccgggacgt cggtgaaacg ccattcgatg agtactatag cattacccga
     1561 tggagcgggc tcaaagcgga gatgaaagat ttttggttcg gcgatcgcgt cgcagccgtg
     1621 cccagcgaag ccgcgcttag tcaaggtaaa tggctctggg atcgggatgg attagccaca
     1681 ccggcgcttc cagaacgaca tacagagaaa accatttatg tccatggcac ggcgccgctt
     1741 accgcctgtg gtatccaaaa gatcgacggg cgatggaaga tgatcggcac gaatgaaggt
     1801 gatggcaccg tcacctggaa atcgggcgcg atcagcggta tcgggcggcg ctattacatg
     1861 ccggcggtac acggcgatct cgctgccaca cccgagtatt ttgccggtat tgcagaattg
     1921 cttcagagcg gcgccaccaa tcaattgcag gatcgggccc ctgaagtgcg cgacccggaa
     1981 gcggccaagc caaagcccta cgaggcgggg ccgccgcgct atgccatgcc ggaggaagca
     2041 gcacatgcgc ttatgggcgc accgaagcga aagagactca aagcgcggcc ggtgtctacg
     2101 ttaaaagtga tcgtcaaggc gatggatctg cgcttcgtca cccagccgat catggtcggc
     2161 cactatgagc aggatgcgat ctcgggcgcg gaatcgatca tcgaccgcga gctcgtcgag
     2221 cacgcattga
//
LOCUS       DASULZ010000040         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1323641, whole genome shotgun sequence.
ACCESSION   DASULZ010000040 DASULZ010000000
VERSION     DASULZ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323641"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..776
                     /locus_tag="VFY96_00775"
     CDS             135..776
                     /locus_tag="VFY96_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005372790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEX5605015.1"
                     /translation="MGTLEALSLAMGTAWTSGINLYATVAALGIANRLELIYLPQNLE
                     VLSHPGVIAIACIMYMIEFFADKVPYLDTGWDALHTFIRVPAGAILAARSLGDMNPAL
                     ELMALLAGGSIALVAHGTKATTRLAINASPEPFSNWVASVGEDVMVFGSIWLMFNHPV
                     IMLIVVILFLAIVAWLAPKIYRLAKRGFHALHNKIRGVKPDQPAPNESSPIPT"
     gene            796..1656
                     /gene="hemQ"
                     /locus_tag="VFY96_00780"
     CDS             796..1656
                     /gene="hemQ"
                     /locus_tag="VFY96_00780"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HEX5605016.1"
                     /translation="MDPLPATAAHPRLPAAPLTLEGAFILHQMFRVRWTAWRDLGSAD
                     RKHVLNRATTTFAAMEANHQEPTALFSLLGHKGDLMLVHFRRTLDELNHAELTVAGSE
                     LNTYLEPTTSYLSVIELGLYEASVKLYSELTAKGLQPSTAEWNREVEAELVQQREAAA
                     PRLWPEIPRRRYLCFYPMNKFRGETKNWYSEPIHERRRMMREHGMIGRHYAGQVTQII
                     SGSIGFDDWEWGVDLFADDPLVFKKLVYEMRFDEASAAYGLFGAFYVGLRFPSAHLES
                     LFDSKTPSFT"
     gene            1689..>2062
                     /locus_tag="VFY96_00785"
     CDS             1689..>2062
                     /locus_tag="VFY96_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5605017.1"
                     /translation="MRLKDKVAIITGAAHGIGKAYARRFAEEGAHVVIADTDHSAGAA
                     VAKALLDSGGSSWARRTDVRSFADVQSLTQETIKRFGRIDVLLNNAAIYVTEKLWKGP
                     VEELAIDEWDRVIEVNLKGVFL"
BASE COUNT          448 a          566 c          557 g          491 t
ORIGIN      
        1 tgccttttcg aaggaataca aagagaaagt cggcggcaag ttcaaaatct aagctcattt
       61 agacgatcgc gaaccgttaa tgttatgttt ctgcaaacac caacgtcaaa cctatagtca
      121 ggaaggagtt tcttatgggc acacttgaag ccttgagctt ggctatgggg accgcgtgga
      181 cctcgggaat caacctgtac gcgaccgtcg ccgcgttggg aattgccaat cggctcgaac
      241 ttatttacct gccgcaaaat ctagaagtgc tatcccatcc gggcgttatt gccattgctt
      301 gtattatgta catgatcgaa ttttttgccg acaaggtgcc ttacctagat accggctggg
      361 acgcgctaca tacgtttatt cgtgtgcccg cgggggcaat tttggcggct cgatcgttag
      421 gcgatatgaa tccggcgctg gaactgatgg cacttcttgc cggcggttcg atcgcgctgg
      481 tcgctcacgg caccaaagcg acaacccgtt tggcgatcaa tgccagccca gagccgttta
      541 gtaattgggt cgcaagcgtc ggcgaggacg tgatggtttt cggcagtatt tggctgatgt
      601 tcaatcatcc ggtaattatg ttgatcgtcg tcatcctctt tttggcaatc gttgcctggc
      661 tagcgccgaa aatttatcgc ctcgccaagc gcggttttca tgcgttgcat aataaaattc
      721 gcggcgtgaa gcccgatcag cctgcgccga acgaatcttc ccctattccg acttaatttt
      781 gcgcccagcc cacgcatgga tcctctaccg gctacggccg ctcaccccag actccccgcc
      841 gcgccgctca ccctcgaagg cgcttttatt cttcatcaga tgtttcgcgt ccgttggact
      901 gcttggcgcg atctcggtag tgccgatcgc aagcacgtct tgaatcgagc gacaaccacc
      961 ttcgccgcca tggaagccaa tcatcaggaa cccacagcac tgttttcgct gctcggccac
     1021 aagggtgatt tgatgctggt gcatttccgc aggactctcg acgagctcaa tcatgcggaa
     1081 ttaaccgtcg ccggatccga gctcaatacc tacctcgagc cgaccacctc ttatctttcg
     1141 gtcatcgaat tgggtttgta cgaagcctcg gtcaagctct attccgaact gaccgcgaaa
     1201 ggattgcagc cgagtacggc agaatggaat cgggaagtcg aagccgagct cgtccagcag
     1261 cgcgaagccg cggcgccgcg gctttggccg gaaatacctc gtcgacgtta tctctgtttt
     1321 tacccgatga ataaattccg cggcgagacg aagaactggt acagcgagcc gatccacgag
     1381 cgccgacgca tgatgcgcga gcacggcatg atcggtcgtc actatgcggg gcaggtcacg
     1441 caaatcatct ccggctcgat cggcttcgac gattgggaat ggggcgtcga tctttttgca
     1501 gatgatccac ttgtgttcaa aaagctcgtt tacgaaatgc gcttcgatga agccagcgca
     1561 gcttatggtt tgttcggcgc attctacgtc ggcctgcgct tcccttcggc acatctggaa
     1621 agcttgttcg acagcaaaac acccagcttc acctgaaaat tcgtggatgg tccgcctgcg
     1681 gaggtccgat gcgactcaaa gacaaagtgg cgatcattac cggcgcggca cacggcatcg
     1741 gcaaggctta tgcgcgccgc ttcgccgagg aaggggccca cgtcgttatt gctgataccg
     1801 atcactccgc cggagcagcg gttgctaaag cattactcga cagcggcggc tcgtcatggg
     1861 cgcgccggac ggatgttcga tcttttgccg acgtgcaatc tttgacgcag gaaacgatta
     1921 aacgttttgg ccggattgac gtcctgctca acaatgccgc aatttatgtc actgagaagc
     1981 tctggaaagg gccagtcgaa gaattagcta tcgatgaatg ggatcgggtg atcgaggtca
     2041 atctgaaagg tgttttcctc tg
//
LOCUS       DASULZ010000041         6230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1120923, whole genome shotgun sequence.
ACCESSION   DASULZ010000041 DASULZ010000000
VERSION     DASULZ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120923"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(239..1255)
                     /locus_tag="VFY96_00790"
     CDS             complement(239..1255)
                     /locus_tag="VFY96_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEX5605018.1"
                     /translation="MMRLVSAKQYAKPFSFLSVILLLSACSLPERKAAYDGWSLAAPL
                     PVPNSETAVASLDGKIYVIGGYPWDRKTVATVQVYDSATDKWQIVAPLPVPLNHVMAA
                     GVGGKIYAIGGQTTESSEPDKAGFVDTVYEFDPGANKWTARAPMPTKRGGGAAAVVDG
                     KIYVAGGRPPGGADFAVYDPKADRWMRLPNLPTQRNHLAADAIDGKIYVVGGRFEGGF
                     RSHRSDAVEVFDPATNQWSKRHPMLRPRGGVNGIAANGCFHLFGGEGNSEHPYGIYPD
                     HHYYNPVTDRWHRLEDRPIAVHGVTGMAFIKGLIHLTGGGTAQGGSSGSTIHQVYKPT
                     MSCK"
     gene            complement(1277..2950)
                     /gene="ilvD"
                     /locus_tag="VFY96_00795"
     CDS             complement(1277..2950)
                     /gene="ilvD"
                     /locus_tag="VFY96_00795"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX5605019.1"
                     /translation="MADNLKPRSSAITDGPDRAPSRAMLKAVGFTDDDLRRPIIGVAN
                     TWIEIGPCNYHLRRLAAKVKEGIRAAGGTPMEFKTVSISDGISMGAEGMKCSLISREV
                     IADSIELVARGNHFDGLVCLSGCDKTNPGVVMALARLDIPGLALYGGSIAPGHLGEKD
                     LTIQDVFEAVGAFASKRIDAEQFLAVENAACPGAGACGGQFTANTMSTVMEFLGISPM
                     GSNGIPATAAEKDEAAFSAGKLVMELLKADLRPSRIITREAIENAIASVAATGGSTNA
                     VLHLLAIAREAGVDLTIDDFDRISERTPLIADLKPGGRFVANDLYKAGGIPLVAKRLL
                     DAELIHGDALTVLGKTIAEAAQMTKETPGQEVIRPLSQPLKQTGGLVILKGNLAPEGC
                     VVKVAGYERMIHKGPARVFDREEDAFKAVQAGKIKDNDVVVIRYEGPKGGPGMREMLG
                     VTAALVGAGLGDSVALLTDGRFSGATHGLMAGHVAPEAAHGGPIAAVRDGDTILFDIQ
                     ARRLDVAIPEEELKARLSRWKAPEPRYKTGVMAKYAKLVSSASEGAVTR"
     gene            3283..3855
                     /locus_tag="VFY96_00800"
     CDS             3283..3855
                     /locus_tag="VFY96_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605020.1"
                     /translation="MISVLAIAVSTTAAPQISREQGNSFARKVDEINQNATAKLATPK
                     RTAMTEAEVNSYLAFNIKEKIPRGLTEPHVRLLGNGQLAGRVLVDMDDFKRGRTSGGM
                     LDPLSYIAGQVPVTARGVLSTRDGKGQFQLIAAEMNGYPLPKPFVQELVRYFSRTPTN
                     PNGFNLEEPFLLPAKIREITVNAAEAVVVQ"
     gene            4090..5085
                     /locus_tag="VFY96_00805"
     CDS             4090..5085
                     /locus_tag="VFY96_00805"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605021.1"
                     /translation="MDIHRSGTLRALFLSLVVFTSPLLAHAEIPVLAGVAGPGINLAY
                     AYLAQDMGFWKKHGLDIKLLLFESGSTLAQVARSGEIKIAINSAPATIAARAQGADSI
                     IVAAAVNTLPYSLVAARGITSWDQLKGKRVGISRFGSGTDTAIRLLCKKFGLDPAKDL
                     IILQGGTQPTRLQALSAGAIDATLVSPPLDFVAKKQGWSIVVNIAQLGIPYPQLVIET
                     TDRLNRESPKLVKGFLAGFFEAIYYSARFKDETKKVITKHLRISDPEILEATYESFIK
                     VTDYSAAPNLEGIRNAIDEVALRVPAVKSKKPQDFVDMRFIQELQSEKFFKQLEN"
     gene            5207..6202
                     /locus_tag="VFY96_00810"
     CDS             5207..6202
                     /locus_tag="VFY96_00810"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605022.1"
                     /translation="MKRTPSIAIAVAALVLALFACPVALPAIEQPIVGVAGPAINMIY
                     AFVARDAGLFQKYGVDPKLVVFDSGSVLAQAALAGDAKISVTSGPVTIASRSQGADAI
                     LVASCVNTPPYSIVAAKNITSFEQLKGKRVAMSRFGSGTDTSLRLVLRKFGLDASKDL
                     MVLQLGTQPSRYQALLAGKIDATIISPPLDLTAKQQGFNILVDIADLGIPYPQQGIET
                     TDRLIRDNPQLVKNFLKGYIEGVYYAAKNKDHTKKIITKYLKTTDPEILEATYRSYVK
                     VTDYSAYPNLEGVQNAMDEVALRVPAVKKKKPADFVNTRFLQELENEGFFKSLSK"
BASE COUNT         1364 a         1838 c         1652 g         1376 t
ORIGIN      
        1 aattccgtcg cgccgcctga ataatttctt ggttgctgac gtaatcgagg ctcatgaaag
       61 gactctcctg ctggagcttt gaaatttttt acatcgcgcc gcagcttatc gaatggcgcg
      121 acaagctgtc aaaggttttg catcaaaaaa gttgcttagg tttaccccgg gattgcgcta
      181 cgcctgatcg atcggcgatt acgcgagcga aagcgatttg aacggcttga atgcagtttt
      241 acttgcagct catcgtcggc ttgtaaacct ggtgaatcgt cgaaccgctg ctaccgccct
      301 gcgccgtccc gccgccggtg agatggatta ggcctttgat gaaagccatg ccggtcactc
      361 cgtgtacggc gatcggccta tcctctaagc gatgccatcg gtcggtcaca ggattataat
      421 aatggtgatc cggatagatt ccgtagggat gttccgaatt gccttcgccg ccgaacaaat
      481 gaaaacagcc gttggcggcg atgccattga cgccgccgcg tggacgcagc atcggatgtc
      541 gcttcgacca ctgattggtc gcgggatcga acacttcgac ggcgtcgctg cgatggctcc
      601 gaaatccgcc ttcgaatctg ccgccgacga catagatctt gccgtcaatg gcgtccgccg
      661 cgagatgatt tctctgcgtg ggtaagttcg gcaacctcat ccaacgatct gcctttggat
      721 catacaccgc gaagtccgcg ccgccgggcg gacggccgcc ggcgacataa atcttcccgt
      781 cgacaaccgc tgccgcgccg ccgccgcgct tggtcggcat cggcgcgcgc gccgtccatt
      841 tattggcgcc gggatcgaat tcgtaaaccg tatcgacgaa tccggcttta tccggctcgc
      901 tcgactccgt cgtctggcca ccgatggcgt aaatttttcc gccaacgccg gctgccatga
      961 catgattgag cggcacgggc agcggcgcga cgatctgcca cttgtcggta gccgaatcgt
     1021 aaacctgcac ggtcgccacg gttttgcgat cccatgggta gccgccgatt acgtaaatct
     1081 ttccgtcgag cgacgccacg gcggtctccg aattcggcac gggcagcggc gcggcgagac
     1141 tccagccatc gtaggccgct ttacgctccg gcagactgca agcactcagt aacaggatta
     1201 cgctcaagaa cgaaaacggt tttgcatatt gcttggcgct tacaagcctc atcatcgccg
     1261 ctccctcatg caaaactcat cgcgtcacag cgccttcgga tgcggaagac acgagcttgg
     1321 cgtatttcgc catcacgcct gttttgtagc gcggctccgg cgctttccat cggctcaacc
     1381 gcgctttcag ttcttcctcg ggaattgcga cgtccagtcg gcgcgcttgg atgtcgaaaa
     1441 ggatcgtatc gccgtcgcgc accgcagcga tcggaccgcc gtgggccgct tcgggcgcca
     1501 catgtccggc catgaggcca tgggtcgcac ccgagaagcg gccatccgta agcagcgcga
     1561 cggaatcgcc caaaccggct ccgaccagcg ccgcagtgac gccgagcatc tcgcgcatac
     1621 ccggcccgcc cttcggccct tcgtagcgaa tcaccaccac gtcgttgtct ttaatcttgc
     1681 cggcctgcac agctttgaat gcgtcttcct cgcgatcaaa cacgcgcgcc ggccccttgt
     1741 gaatcatgcg ctcatatccc gcgactttga ccacgcagcc ttccggcgcc aagttgcctt
     1801 tgagaatcac caagccgccg gtttgcttta acggttgcga tagtggccgg atcacttctt
     1861 gccccggggt ttccttggtc atttgcgccg cttcggcaat ggtctttccc aaaaccgtca
     1921 gcgcgtcgcc atgaatcaat tcggcatcca gcaaacgctt ggcgaccagc ggaatacctc
     1981 cggccttgta aagatcgttg gcgacaaaac gaccgcccgg cttgagatct gcaatcagcg
     2041 gcgttcgttc gctgattcga tcaaaatcat cgatggttaa atccacgccg gcttcgcgcg
     2101 cgatcgccag caagtgcagc acggcattgg tcgaacctcc cgtggccgcc accgatgcga
     2161 tcgcgttttc aatcgcctcg cgcgtgatga tgcgcgaagg tcgcaggtcc gctttgagca
     2221 attccatgac cagtttgcca gcgctgaacg cagcttcatc tttttccgcg gcggttgcgg
     2281 gaattccgtt gctgcccatc ggtgagatac cgagaaactc catgaccgtc gacatcgtgt
     2341 tcgccgtaaa ctggccgccg caggcgcccg ctccggggca ggcggcattc tcaaccgcca
     2401 agaattgttc cgcgtcgatg cgcttgcttg cgaaagcgcc gaccgcttcg aagacgtctt
     2461 gaatggtcaa atccttttcg cccaaatgtc ccggcgcaat ggagccgccg tagagcgcta
     2521 acccgggaat atcgagccgc gcgagcgcca tcaccacgcc tggattcgtc ttgtcacagc
     2581 cggacaaaca aaccagccca tcgaaatgat tgccgcgcgc cacgagctcg atggaatcgg
     2641 cgatcacctc ccggctgatc agcgagcatt tcatgccttc cgcgcccatg ctgatgccgt
     2701 cagaaatcga aaccgtcttg aactccatcg gtgtgccacc ggcggcgcga atcccctctt
     2761 tcaccttcgc cgcgagccgg cgcaagtgat agttgcacgg cccgatctcg atccatgtgt
     2821 tggcgacgcc gatgatcggc cggcgcaaat cgtcgtcggt aaaccccacc gctttgagca
     2881 tcgcgcggct cggtgcgcga tccggcccat cggtgatcgc tgagctccgc ggcttcaaat
     2941 tatccgccat aattcgctcc taaactcact ttgaatgggc ttcacttaac tatgtgaaag
     3001 caacatttgc aagccaatga tcgaggagga gcggttcggc atgaagacca cgaggttacc
     3061 ccctgagtgt gagacctttc gaggtgacaa accgattttt tggtcttcct taacacgcaa
     3121 gtaaggaatt tttcccagcg gagtttacaa aaaagctaaa ctgaacaagt acactgcggc
     3181 atacgcaaca atggcgtcta tgaactggta attgtaatga ccctgctcaa tctttaaaat
     3241 tcttccatgc gacggatttc ttttagctcg gtcgtgtgtg tcttgatttc ggttctagcg
     3301 attgccgttt ccaccaccgc ggcgccgcag atttcgcgcg aacaaggcaa cagtttcgcc
     3361 agaaaggtcg atgagatcaa tcaaaacgcc accgcaaagc ttgcaactcc gaagcgcaca
     3421 gcgatgaccg aagcggaagt aaactcctat ctggctttca acatcaagga aaaaattccc
     3481 cgcggtttga ccgagccaca cgtccgtttg cttggcaatg gccaacttgc cgggcgggtt
     3541 ctcgtcgata tggatgattt caaacgcggt cgaacctcgg gcggcatgtt ggacccgctg
     3601 agctatatcg ccgggcaagt gcccgtcacc gcgcgcggtg ttctcagcac gcgagacggt
     3661 aaagggcaat ttcaattgat tgccgcagag atgaatggct accctttgcc aaagccgttt
     3721 gtgcaggagc tcgtgagata tttttcccgc acgccgacta atcccaatgg attcaacctc
     3781 gaagagccct tcctcttacc ggcgaagatc cgcgagatca cagttaacgc agccgaagcg
     3841 gtcgtcgtcc agtaatcaca ccggagccca tcgtactcgt cagattcggt ttgccaggca
     3901 cgaaatcgcc tggatctcac aacccggcac accacgaacc gggtttgatg gaatttctta
     3961 agctttcggg tagccgctcg gtacgctaga atggataagc gaaggctcgg gcgaccaaac
     4021 ggccgccgcg cgcatctaag agcagaccac ccgggttaac gccggcgtaa cgaaaggaac
     4081 aaagacatct tggatataca tcggagcgga acgttacgcg cgctgttttt gtcacttgtc
     4141 gttttcacct cgccgctact agcccatgcc gagatacccg tcctggccgg tgtcgctggt
     4201 ccaggtatca atttggctta tgcatacctc gcccaagata tgggattttg gaaaaagcac
     4261 ggcctcgata tcaagcttct gctcttcgaa tccggctcga ctctggcgca ggtcgctcgt
     4321 tctggcgaga tcaagatcgc tatcaattcg gcgccagcta cgatcgccgc tcgtgctcaa
     4381 ggcgctgatt cgatcatcgt tgccgctgcg gtgaatactc tcccgtatag tttggtggcc
     4441 gcgaggggaa tcaccagttg ggatcagctc aaaggaaaaa gggtcggtat cagccgtttc
     4501 ggctccggga cggacacagc gattcgcttg ctatgcaaaa agttcggact cgacccggcg
     4561 aaggatctca tcatcttgca aggcgggaca cagccgacaa ggctccaagc gctatcggcg
     4621 ggggccatcg atgccacgtt ggtttctccg ccgttggact tcgtcgccaa aaagcaagga
     4681 tggtctattg tcgtaaacat cgcgcaattg ggcattcctt atccgcaact cgttatcgaa
     4741 accactgatc gtttaaaccg cgagagtcct aagctcgtga aaggttttct cgcgggtttc
     4801 tttgaggcga tttactatag cgccagattt aaggatgaga ccaaaaaagt gattaccaag
     4861 catctaagaa tctccgatcc ggaaattctt gaggcgacct acgagagctt catcaaagtg
     4921 acggactata gcgccgcacc caatctcgaa ggcatccgca atgcgatcga cgaagtggcg
     4981 ctgcgcgtcc ccgcagtgaa gagcaagaaa ccccaagact ttgtcgatat gcggtttatt
     5041 caagaattgc agagcgaaaa atttttcaag cagcttgaaa attgattatt ttccaacgac
     5101 ggcttcgccg gcgggattcg aacttttagc tgatcgacag gcctgctctc tcgctattga
     5161 attcccttgc ttggttcgct ataacccaat ccgccatgag gcccgtgtga agcgcacccc
     5221 ttcgatagcc attgcagtag ccgctctcgt tttagctctc ttcgcctgcc ccgttgctct
     5281 tcccgccatc gagcaaccca tcgtcggcgt tgccggtccg gcgatcaaca tgatttatgc
     5341 cttcgtcgcc cgggacgcag gcttgttcca aaagtatgga gtcgatccca agctcgtagt
     5401 cttcgattcc ggctcggtgc tagctcaagc tgcgctcgcc ggcgacgcaa aaatctccgt
     5461 gacctcaggc ccggtaacaa tcgcttcccg gagtcaaggc gccgacgcga ttctggttgc
     5521 gagttgtgtg aacaccccgc cctacagcat cgttgcagca aagaacatta ccagtttcga
     5581 gcaactcaaa ggaaagcgcg tcgccatgag ccgtttcggt tccgggacgg acacctcgct
     5641 tcggctggtg ctcagaaaat tcggactcga tgccagtaag gacctcatgg tattgcaact
     5701 cggcactcag ccgagccgat atcaggctct gctggccggt aagatcgatg ctacgatcat
     5761 ttcgcctcca ctggatctca cagccaagca gcagggtttc aacattttag tggatatcgc
     5821 agacctcggc atcccatatc cgcagcaagg catcgagacc accgatcgtc tcattcgcga
     5881 caatccacag ctggtaaaga actttttgaa gggctacata gagggcgtgt actatgccgc
     5941 gaaaaacaag gaccacacga aaaaaattat tacgaaatat ttgaaaacca ccgatccgga
     6001 aattctcgag gcgacgtatc gaagctatgt aaaggtgacg gattacagcg cttacccgaa
     6061 tctggagggg gttcagaacg cgatggatga ggtcgcgctc cgggtgccgg ccgtgaaaaa
     6121 gaaaaaaccg gcagatttcg tgaatacgcg ctttttacag gaactggaaa acgaggggtt
     6181 ttttaagtcg ctcagcaaat agtcgtcttc tctgctgctt cgatagagta
//
LOCUS       DASULZ010000042         3586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_917836, whole genome shotgun sequence.
ACCESSION   DASULZ010000042 DASULZ010000000
VERSION     DASULZ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917836"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2130)
                     /locus_tag="VFY96_00815"
     CDS             complement(<1..2130)
                     /locus_tag="VFY96_00815"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5605023.1"
                     /translation="MPKVNVVVRWRGRNGVVMTAVGLSLVRIDGIDKVAGRAMYAGDL
                     HLPGMAIAKVLRSPVPHARVRRIDATRALALQGVLAVLTRDNLDVAGSSFGAYVRDQE
                     ILACEKVRYVGDMVAAVAATEDGVAAEALKLIDVDYEELPAVFTIDDALKNGAPLVHD
                     SLDNRKDPGYGRGGSHIVHDQSNICFHFRHERGDVEEGFRQSDQIFEDVFHFPSAQHY
                     PMEPHLCVAHFEAGVLTVWSATQSPFPVRQELARIFGLPFSAVRVIVPHVGGGYGAKS
                     GIKTEAIAACLSRRIGRPLKLAFGADETFRTICQPRAKLVVKTGVKKDGTFIARRCEV
                     YLNGGAYANSGPTVAEKAGYRAHGPYRIPHVLTDAYSIYTNTVPGGAFRGFGGPQVAF
                     AYETHIDMIVNRLGLDAIEIRMKNLLDKGESFSYSDTPIDCDLKAGLRSIAQAIGWDS
                     RPNGEPQPDSKRRGIGIATAMKDGGGTNKPANAAVTIFNDGSVLLSTGSVEIGQGMRT
                     AFLQIVAEELAVATDSVRVAALDTHYTPFDKGTNASSATSVMGQAVQRAARDARRQFL
                     EAASAVLNAPSSEIRLDNGFASHGTETVSFREIMQRHFRDSEGEIWGRGYFKVARNEN
                     VPLGYLSPFWEIGFGAAEVEVDSETGEVKILRYVSLTDAGKMINPLQCEAQDEGAAVF
                     GLGQALFEDLAYQDGQLLNGTLIDYRLP"
     gene            2188..2925
                     /locus_tag="VFY96_00820"
     CDS             2188..2925
                     /locus_tag="VFY96_00820"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX5605024.1"
                     /translation="MADHSKLSAQEAKQKTEAIRKVILDTYHREVKPSPFFTDLRAGT
                     LSRERLQGWIKNWYSFALEVNTGMATVYHQFVGFFKRHPELEDRIAQKIGEEFTTPTI
                     GGHARMLPVLGKALGVSAEEMIECELIPEARGLVDFFVRSWIDIPLAECFAVHMEEEI
                     FGFISRDFRQSLPKYGVSNEAMKYFRVHEEADLDEDHGGTNQAILEVALQEGYGNERA
                     GWPLEYCGKIAVQMIGNFYDGVYRRFP"
     gene            3050..>3586
                     /locus_tag="VFY96_00825"
     CDS             3050..>3586
                     /locus_tag="VFY96_00825"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5605025.1"
                     /translation="MEQPKEIQELNETLRPRWLSGLWNVDHAERPSDPKTRVKPHLWK
                     WDDVYDSLLQAREKISVASGSVERRVIRLVNPGLAATEMTSHTILLSFQLIQPGEIAP
                     AHRHTMSAFRFILQGHGAYTNVDGEKMVMEDGDLILTPHGCWHEHAHEGADNMVWIDG
                     LDVPFVQALQQISFDPYKQ"
BASE COUNT          788 a         1050 c         1002 g          746 t
ORIGIN      
        1 cggcaagcga taatcgatca acgtgccgtt gagcagctgt ccatcctggt aggcgagatc
       61 ttcgaacaac gcctggccaa gaccgaaaac cgcagcgccc tcgtcctgag cctcgcattg
      121 cagcggatta atcattttgc ccgcgtcggt gagcgacacg taacgcaaaa tcttcacctc
      181 tcccgtctcg ctgtccacct caacctcagc cgcgccaaag ccgatctccc agaacggcga
      241 gaggtaacca agcggaacgt tttcattcct agcgactttg aagtatccgc gcccccaaat
      301 ctcaccttcc gagtcgcgga agtggcgctg catgatttcg cgaaagctga ccgtttcggt
      361 gccatgtgac gcaaagccgt tgtccaggcg aatttcgctg gatggcgcgt tcaagacggc
      421 gcttgccgct tcgagaaact gccggcgcgc atcgcgcgcg gctcgttgca cagcctgacc
      481 catgaccgac gtggcgctgc tcgcattggt gcctttgtcg aacggcgtat aatgcgtgtc
      541 caacgcggca acccgcacgc tatcggtggc gaccgccaac tcctcggcaa cgatctgcag
      601 gaaggcggtg cgcatgcctt gaccgatctc caccgaaccg gtcgacaata gcacgctgcc
      661 atcgttgaaa atcgtcaccg cggcattcgc aggtttgttg gtgccgccgc cgtctttcat
      721 tgccgtcgcg atgccgatac ctcttcgctt ggagtcaggt tgcggttcgc cgttcgggcg
      781 gctatcccag ccaatcgctt gcgcgatgct cctcaggccg gccttgagat cacaatcgat
      841 cggcgtatcg ctgtaggaaa aactttctcc tttgtcgagc aaattcttca ttcggatctc
      901 gatggcgtcc aggccgagcc gattcacgat catgtcgatg tgggtttcgt aggcaaacgc
      961 cacctgcggt ccgccgaagc cgcgaaaagc gccgcccgga accgtgttgg tgtaaatcga
     1021 ataagcgtcg gtcaggacat gcggaatacg gtatggaccg tgcgcgcgat agccggcttt
     1081 ctccgctacc gtcgggccgg agttggcata agcgccgcca tttaaataaa cctcgcagcg
     1141 gcgcgcgatg aacgtgccgt cttttttcac gccggtctta acgaccagct tagcgcgtgg
     1201 ctggcaaatc gtcctgaatg tctcatcggc gccgaaagcg agcttgagcg gacggccaat
     1261 ccggcgcgac agacaggccg cgatcgcttc agttttaatg ccgcttttgg cgccgtaacc
     1321 accgccgacg tgaggaacga tgacgcggac cgcgctgaac ggcaaaccga aaatacgcgc
     1381 gagctcctgg cgaaccggaa atggactctg tgtcgcgctc cacacggtga gtacgccggc
     1441 ttcgaagtga gccacgcaaa ggtgcggctc catcggatag tgctgggcgc tgggaaaatg
     1501 aaaaacgtct tcaaagatct ggtcggattg gcgaaaaccc tcttcgacgt caccccgctc
     1561 gtgacgaaaa tgaaaacaga tattgctctg atcgtggaca atgtggctgc cgccccggcc
     1621 gtaacccggg tccttacggt tatcgaggct gtcatggaca agcggcgcgc cgtttttcaa
     1681 cgcgtcatcg atggtgaata ccgccggcag ctcttcgtag tcgacgtcaa tcagcttcag
     1741 cgcctctgcc gcaacaccat cctcggtggc agcgaccgcg gccaccatgt cgccaacata
     1801 acggactttc tcgcatgcca agatttcctg atcacggacg tatgctccga acgagctccc
     1861 tgcgacgtcc aaattgtccc tggtgagcac cgcgagcact ccttgaagag caagcgctct
     1921 ggtcgcatca atacggcgaa ctcgggcgtg cggcaccggg ctgcgtaaga ctttggcgat
     1981 cgccatacct ggcaagtgca ggtcgccggc atacatcgca cggccggcga ctttatcgat
     2041 gccatcgatt cgaaccaagc tgagacccac ggccgtcatg acgacgccat ttctgccgcg
     2101 ccatcggacc acaacattga ctttcggcaa agccttcggc taaagatcgt ggtattaatc
     2161 atggtcgcac tcgaaaggat tttgtccgtg gctgaccata gcaaactgag cgcgcaagaa
     2221 gcaaaacaaa agaccgaggc aatccggaaa gtcattctcg atacctacca tcgcgaagta
     2281 aaaccctcgc catttttcac cgatttgcgt gccgggacat tgtctcgcga acggctccaa
     2341 ggttggatca aaaactggta ttcctttgcc ctcgaagtga ataccggaat ggccacggtc
     2401 tatcatcagt tcgtcggctt ttttaaacgc catccagagc tcgaagaccg cattgcgcaa
     2461 aaaatcggcg aagagttcac gactccgacg atcggcggcc atgcgcgcat gctgccggtc
     2521 cttggcaaag ctctcggcgt ctccgctgaa gagatgatcg agtgtgagct cattccggaa
     2581 gcccgcggct tggtcgattt ttttgtccgc tcctggatcg acattccgct cgccgagtgc
     2641 ttcgccgtgc acatggaaga agaaattttc ggtttcattt cacgggactt ccgccaaagc
     2701 ttgcccaaat acggtgtcag caacgaagcg atgaaatatt tccgcgtgca cgaggaagcc
     2761 gaccttgacg aagatcatgg cggcacgaac caggcgattc tggaagtcgc gctccaggag
     2821 gggtacggca acgaacgcgc cggttggccg ctggaatatt gcggcaagat cgcggtgcag
     2881 atgatcggca atttttacga tggggtttat cgacggttcc cttgagcgaa gtaagggttc
     2941 aaaccgtgca gtccgtcggt ccagacgctt tctagtgtag gccgatttga acgtgttaaa
     3001 cgggggcgaa acggttgttg aacggtttga acgaggtcag aggtgaacga tggaacagcc
     3061 caaagaaatc caagagctga atgaaacgct tcgtccgcgc tggctttcgg gactgtggaa
     3121 tgtcgatcat gcggaacgtc cgtccgatcc gaagacgcgg gtaaagccgc atctttggaa
     3181 atgggacgat gtctacgaca gtctgcttca ggccagggaa aaaatttctg ttgcgagcgg
     3241 cagcgtcgag cgacgcgtca ttcgactggt gaacccgggg ctcgccgcaa cggagatgac
     3301 cagccataca attctactat cgtttcagtt gatccaaccc ggcgagatcg ctcccgcaca
     3361 tcgccatacc atgagcgcgt ttcgtttcat tctccaaggc cacggcgcct ataccaatgt
     3421 cgatggtgaa aagatggtta tggaagatgg cgatctgatt ctcacgccgc acggctgctg
     3481 gcacgaacac gcccacgaag gcgctgacaa catggtctgg atcgacggcc tcgacgtccc
     3541 ctttgttcaa gcgttgcagc agatctcttt cgatccctac aaacag
//
LOCUS       DASULZ010000043         6301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_106240, whole genome shotgun sequence.
ACCESSION   DASULZ010000043 DASULZ010000000
VERSION     DASULZ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6301
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106240"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VFY96_00830"
     CDS             complement(<1..655)
                     /locus_tag="VFY96_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018174705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX5605026.1"
                     /translation="MKLSVIVPVLNEADVIGANLEALLAVAPYEIIVVDGGSTDATRQ
                     VCHQLGVKVVTCERGRGHQMNVGASQASGDVLLFLHADTRLPSCALGDIATALRDSRC
                     PGGRFDLEIDGMHWMLKIVAALINHRSRLTQVATGDQAIFVRRTVFQSVGGFQDVPLM
                     EDIAFSRALKQIGPIACLRSRVITSGRRWESDGVWRTIVKMWALKLAYLAGVSPHRLK
                     "
     gene            complement(673..1521)
                     /locus_tag="VFY96_00835"
     CDS             complement(673..1521)
                     /locus_tag="VFY96_00835"
                     /inference="COORDINATES: protein motif:HMM:NF016658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HEX5605027.1"
                     /translation="MKAALRINLVLALILWLTSVDSVGQSPSRIDLWARWDKHDAAST
                     ETIDHTRWQGFLQKFVVTAHPSGINRIRYQAVGADAFASLRGYLSSMQTVTISNYNRA
                     EQKAFWINVYNALTVQLVLSRYPIASIRDINISPGLATRGPWGAKLFIVEGERLTLDD
                     IQHRILRPIWKDSRAHYALTTAALGSPHLQSIAYTARNSEALLEKSAREFINHPRGVS
                     IQNGRLQVSTLYVWFQEDFGGGAEGLMEHWQDYADASLADALAKYNGGLAHDYDWRLN
                     GSESKP"
     gene            complement(1532..2281)
                     /locus_tag="VFY96_00840"
     CDS             complement(1532..2281)
                     /locus_tag="VFY96_00840"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEX5605028.1"
                     /translation="MNFRRAVLLVIVVTAVAVGFLLLPAREWFSQFEAYVASLGVLGP
                     FLFAFTYMVLTVLLFPASILTIGAGTLFGLKTGFFAALLGANLGAFCAFLLARTFLRE
                     RVVLWTASHPKFRFLDEAIAKQGFKMVLLCRLSPIFPFILLNYFLGLTSVRSAAYVLA
                     NLLGMVPANFVFVYIGATARDAVGGSVESSLGFYQQLLKYIGLLATLAAFIVITRIAR
                     RALRDAERAQEGGGFVAPSSCGVPPPQGEGE"
     gene            complement(2385..3344)
                     /gene="arsS"
                     /locus_tag="VFY96_00845"
     CDS             complement(2385..3344)
                     /gene="arsS"
                     /locus_tag="VFY96_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020482782.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HEX5605029.1"
                     /translation="MQPFSEKIRQLGAPLTRRGVDALQVNLGRYCNLACIHCHVESGP
                     TRTEIMSRETVDAVLAFLARSSIPTLDLTGGAPELHPDFDYLIESARAMDRHVMDRCN
                     LTVVFEPGKDYLPEFFKRHQVELICSLPCYSEENVDRQRGKGTFDLSIRALQRLNEIG
                     YGQPGSNLLLNLVYNPVGPHLPPPQEQLEEEYKQKLGAEFGIVFNHLFCLTNMPITRY
                     AKHLRLRNEYDRYQDLLEENFNGATLDHMMCRNLISIGWHGSVYDCDFNQMLDLALTN
                     DAGERLNIASLAPEVIERGAIRIGDHCYACTAGPGSSCGGALV"
     gene            complement(3367..4471)
                     /gene="prfB"
                     /locus_tag="VFY96_00850"
     CDS             complement(join(3367..4410,4412..4471))
                     /gene="prfB"
                     /locus_tag="VFY96_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157212186.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX5605030.1"
                     /translation="MLEETAEQLTHLEQRLDLLRGVFDVESKQKRIEELNQITSQPDF
                     WNDAEKAQAIFKEQSALKAVIDAWAKHRAELEEARFFLDVAKDEKSEDALAEAATKVA
                     AVGKEMAETELKQLLGGPDDRRNAIVNLHPGAGGTEAQDWAEILLRMYLRWCDRRGYR
                     KEILEYQPGEEAGVKSVTFLVEGDYAYGYLKAEAGIHRLVRISPFDANSRRHTSFASV
                     FVYPEVDDDIKIEINEADLRVDTYRSSGAGGQHVNKTDSAVRLTHLPTGIVVACQNER
                     SQHKNRAMAMKILKSRLYELEVEKQKEKMESYHKTKKDIAWGSQIRSYVLAPYRMVKD
                     HRTNVEVGNADAVLDGDLDQFIQAYLMQADEPA"
     gene            complement(4514..4963)
                     /gene="ybeY"
                     /locus_tag="VFY96_00855"
     CDS             complement(4514..4963)
                     /gene="ybeY"
                     /locus_tag="VFY96_00855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX5605031.1"
                     /translation="MPVEIAQRGAGKKFSTGKIKKIALIILELVAEKNAELSLALIGD
                     VEMKKLNAKYRNKDYPTDVLSFPIEGVVPRETRLLGDVIISVEKAAEQAKARGHSSEQ
                     ELITLLIHGVVHLLGYDHERSVREARAMRRVEKRIYRQLCERGLIKV"
     gene            complement(5030..6109)
                     /locus_tag="VFY96_00860"
     CDS             complement(5030..6109)
                     /locus_tag="VFY96_00860"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5605032.1"
                     /translation="MSLKGILLAAQISLCAWIVSADFGHSATIDDVVKSIRGVPALQR
                     KTILEEGARKEGEVIWYTSMSLTDFPKIVGAFEKTSPHVKIRTNRLSQSSVLPKIETE
                     ARAGHYAVDVVGSAPMEMWELKQKGYSAAYLSPELKAFPAGSFDPQGFWSSTEVTPLV
                     LALNTNLVSPDEAPRNYQDLLQPRWKGKMNFGSDEYAWYSVMLDGMGKAKGLEYMKAL
                     ARQQLHIPGGSSIMRLQLMLAGESALAIAARGRRATEYKEKGAPIDYRLLDPYPAEPN
                     GLALMRRSAHPHGSILFIDWMLSEEGQTVLAQQIPRITLRKGIKQIPRHQELYKKEFV
                     FVNPAFIGPNLNDLIASYQQIFNIR"
BASE COUNT         1401 a         1736 c         1741 g         1423 t
ORIGIN      
        1 gtttgaggcg gtggggcgaa acgccggcta gataagcgag cttcagcgcc cacatcttaa
       61 cgatcgtccg ccaaactccg tcgctttccc agcgccggcc ggaggtaatc acgcggctac
      121 gcagacaagc gatggggccg atctgtttga gcgcccggga gaacgcaatg tcctccatca
      181 gcggcacatc ttggaatccg cccacgctct ggaacaccgt gcggcggacg aaaattgcct
      241 gatcgcccgt cgcgacttgg gtgaggcgag accggtgatt gatcagggcc gcgacaattt
      301 tcagcatcca gtgcataccg tcgatctcga gatcgaagcg gccgccggga cagcgtgagt
      361 cgcgcagcgc ggtggcgata tctcccaacg cgcagctggg caatctggtg tccgcatgga
      421 gaaacagcag cacatcgccg ctggcttgac tcgcacccac attcatctgg tgccctcggc
      481 cgcgttcgca tgtcactacc ttgacgccga gttgatggca aacttgtcgg gttgcgtcgg
      541 tgctgccccc atcgacaaca atgatctcgt acggggcgac cgcgagcagc gcttctaagt
      601 tggcgccgat gacgtctgct tcgttgagta cgggaacgat tacggaaagt ttcaactggg
      661 ctacacgatg ttctatggtt tcgattcgct gccgttcagg cgccagtcat aatcatgggc
      721 taagccgccg ttgtattttg ccagtgcgtc ggccagggat gcatccgcgt agtcctgcca
      781 atgttccatc aagccctctg cgccgccgcc gaaatcctcc tgaaaccaaa cgtaaagagt
      841 cgagacctgg agccggccgt tttgtatgga gacaccccgc gggtgattga tgaattcacg
      901 agcggatttt tccagcaagg cttcgctgtt tcgcgccgtg taggcgatcg attgcagatg
      961 aggggagccg agggcagcgg tcgtcagcgc gtaatgggcc ctgctgtctt tccaaatcgg
     1021 tcggaggata cgatgttgga tatcatcgag cgtcagtctt tcaccttcca cgatgaacaa
     1081 cttggcgccc cagggaccgc gcgtggcaag accgggtgag atattgatgt ctcggatcga
     1141 agctatgggg tagcgggaca agaccaattg caccgtaaga gcgttataaa cattaatcca
     1201 gaaagctttc tgttcggcgc gattgtagtt cgagatcgtc accgtctgca tcgatgaaag
     1261 gtaaccgcgc aaactggcaa aagcgtcggc gcctacagct tgatagcgaa tccgattaat
     1321 tcccgacgga tgagccgtaa caacgaattt ttgcagaaac ccttgccatc ttgtgtgatc
     1381 aatcgtctcc gtacttgccg catcgtgctt gtcccaccgc gcccaaaggt cgatcctcga
     1441 aggagactgt cccacggaat cgacactggt aagccacagg atcagtgcca aaactaagtt
     1501 gattctcagg gccgccttca tctttatcgc ttcattctcc ttctccctgg ggtggcggga
     1561 caccgcagga actcggcgcg acgaaaccgc cgccctcctg cgctcgctca gcgtcgcgca
     1621 gcgcgcggcg cgcaatgcgc gtgatcacga taaaagctgc gagtgtcgcc aggagtccga
     1681 tgtacttcag tagttgttgg taaaatccga gcgaagattc cacagatccg cctactgcgt
     1741 cacgggcagt ggcgccgatg tagacgaaga cgaaattcgc cggcaccatt ccgagtaagt
     1801 tcgccagaac ataagcggcg ctgcggacgg aggtcagacc gagaaagtaa ttgagcagaa
     1861 tgaatggaaa aatcgggctc aagcggcaga gcaaaaccat cttgaagccc tgcttagcga
     1921 tggcttcgtc gaggaaacga aatttcgggt gcgaagcggt ccagagcacg actcgttcac
     1981 gcaaaaacgt gcgcgccagc aggaaggcgc agaaggcgcc tagatttgcg ccgagtagcg
     2041 ccgcaaagaa tcctgttttc agcccaaata acgtgccggc gccgatcgtc agaatgcttg
     2101 ccggaaacag aaggacggtc aggaccatat aggtgaaagc aaacaaaaat ggtccgagca
     2161 cgccgagaga agcgacataa gcctcgaatt gcgaaaacca ctcgcgcgcc ggcagcagca
     2221 aaaagccaac tgccactgca gtgacaacaa tgaccagaag tactgctcga cggaaattca
     2281 tcgagttccg gactggtaaa aagaaccaag atagatcgcg cagaacgaag gaaagaaccg
     2341 tttcgcccta tgatggacgg ctgctacgac cctgcaaccg gggctcaaac cagagctcca
     2401 ccgcaactgc tccctgggcc agccgtacag gcgtagcaat gatcgccgat gcgaatcgct
     2461 cctctctcta ttacttcagg cgccagtgaa gcaatattga gtcgctcgcc ggcgtcgttg
     2521 gttaaagcaa gatcgagcat ctgattgaag tcgcagtcat aaaccgagcc gtgccaaccg
     2581 atgctgatca gattgcggca catcatatga tccagggtgg cgccgttgaa gttttcttcg
     2641 agtaaatctt gatagcgatc gtattcattg cgcaatctga gatgtttagc atagcgcgta
     2701 atcggcatat tcgtcagaca gaagaggtga ttgaaaacga taccgaactc cgcgcccaac
     2761 ttttgtttgt attcctcctc gagctgctct tggggtggcg gcagatgtgg acccacggga
     2821 ttataaacga ggttcaacag aagattgcta ccgggctgac cgtagccgat ttcgttcaag
     2881 cgctgcaaag cgcggatgct caaatcgaat gtccctttgc cgcgttgccg gtcaacgttt
     2941 tcttcggaat agcagggcag ggagcagatc aactctacct gatggcgttt aaaaaactcc
     3001 ggcagatagt ccttgcccgg ctcgaagacg acggtcaagt tacagcgatc catcacatgg
     3061 cgatccattg cacgtgccga ttcgatcaga tagtcgaaat cggggtgtag ctcgggcgcg
     3121 ccgccggtga gatccaaggt gggaatgctg ctgcgggcga gaaacgcaag cacggcatcg
     3181 acggtctcgc ggctcatgat ttctgtgcgc gttggaccgg attcgacgtg gcaatggatg
     3241 cacgccaggt tacagtaacg gccgagattc acttgcagcg catccacccc gcgccgggtc
     3301 agcggcgcgc cgagctggcg aatcttctca ctaaacggct gcacaaatac cactccgggc
     3361 caggcgctac gccggctcgt ccgcctgcat cagataagcc tgaatgaatt gatcgaggtc
     3421 gccgtcgagc acagcgtcgg cattgccgac ctcgacattg gtgcggtgat ctttgaccat
     3481 tcggtacgga gcaagcacat aggagcggat ctgactgccc caggcgatgt ctttttttgt
     3541 tttatggtag ctctccattt tttctttttg cttttcgacc tcgagctcgt aaagtcgcga
     3601 cttcaagatc ttcatcgcca tggcgcggtt tttgtgttgc gagcgctcgt tctgacacgc
     3661 aacgacaatc ccggtcggaa gatgggtcaa acgcacggcg gagtcagttt tgttgacgtg
     3721 ctgcccgccg gcgccgctcg agcgataggt atcgacccgc aggtcggcct cgttgatctc
     3781 gattttgatg tcgtcgtcca cttcgggata gacaaaaacc gacgcaaacg aagtatgccg
     3841 ccgcgaattg gcgtcgaacg gcgagatacg cacaagccgg tgaatcccgg cttcggcttt
     3901 taaatagccg taggcatagt cgccttccac caagaaggtc acgcttttca cacccgcctc
     3961 ttcgccgggt tggtattcga ggatctcttt ccgataacca cgccggtcgc accagcgcag
     4021 atacatgcgc aataatattt ccgcccaatc ttgcgcctcc gtgccgccgg cgccgggatg
     4081 aaggttgacg atggcattgc gccggtcgtc aggaccgccg agcaattgtt tgagctcggt
     4141 ttcagccatc tctttgccaa cagcggcgac ctttgttgcg gcttcggcaa gggcatcctc
     4201 gcttttttca tctttggcga catcaagaaa aaatctggcc tcttcgagct cggcgcgatg
     4261 ctttgcccat gcgtcgatga ccgctttgag cgcagattgt tctttgaata tcgcctgggc
     4321 tttttctgcg tcgttccaga aatcgggctg ggaggtgatc tggttcagct cctcgatccg
     4381 tttttgcttg ctttcgacgt caaagacgcc tccgcaggag atcgagacgc tgttcgaggt
     4441 gagttaactg ttctgctgtt tcttccaaca ttgctttttc gctttcgaag gaacgatcat
     4501 tcaccgctgg aaattatacc ttgattagtc ctcgctcaca aagctggcga tagattcttt
     4561 tttccacccg acgcatggcg cgggcttccc gcaccgagcg ctcgtggtcg tagcctaaca
     4621 ggtgaacgac gccatgaatc aacagggtga tcaactcctg ctcagagctg tgaccgcgcg
     4681 ccttcgcttg ttcggcggct ttctcgactg aaatgatgac atctcccaac aatcgagtct
     4741 ccctcggcac gacgccctcg atcgggaacg acaaaacatc ggtaggataa tctttgttgc
     4801 gatatttcgc attgagcttt ttcatttcca catcgccgat caaagccagg ctgagctcgg
     4861 cgtttttctc tgcgaccaac tccaggatta tgagcgcaat tttttttatt ttaccggttg
     4921 aaaatttttt gccggcgcca cgctgcgcga tctcgacagg catttgtcga agcttaacgg
     4981 tttgctcgaa ccgtcgcgaa tgatagacga ctgccgctcc tcatccagtt tagcggatat
     5041 taaaaatctg ctgatacgaa gcgatcagat cattcagatt ggggccgata aaagcggggt
     5101 taacgaagac gaattctttt ttataaagct cctgatgcct gggaatttgt ttgatgcctt
     5161 tgcgcagggt gatgcgcgga atttgctgcg ccaaaacagt ttgcccctct tccgataaca
     5221 tccagtcgat gaaaagaatc gatccatggg gatgtgcgga gcggcgcatc agggccaggc
     5281 cgttcggttc ggcggggtac gggtcgagca ggcggtaatc gatcggcgcg cctttttcct
     5341 tgtattcagt ggcgcgccgc ccccgcgcag cgatagcgag cgccgattca cccgccagca
     5401 tgagctggag ccgcatgatg ctgctgccgc caggtatatg gagctgctgg cgcgctaagg
     5461 ctttcatgta ctcgagccct ttggctttgc ccatgccatc gagcatcact gagtaccacg
     5521 cgtactcatc actgccgaaa ttcattttac ccttccatct cggttgcagc aggtcttgat
     5581 agtttcgcgg tgcctcatcc ggagatacca aattcgtatt caaggccagc accagcggcg
     5641 tcacttcagt cgatgaccaa aatccttgcg ggtcgaagga accggcgggg aaagccttca
     5701 actcgggcga aaggtaagct gcggaataac ctttttgttt tagctcccac atctccatgg
     5761 gtgcggagcc cacgacgtcg acggcataat gcccggcccg cgcttcggtt tcgattttgg
     5821 gcagcaccga tgactgcgat agacgattgg tgcgaatctt gacgtgagga gatgtctttt
     5881 cgaaggcgcc gacaattttc ggaaaatcgg tgaggctcat cgaggtatac cagatcacct
     5941 ccccttcttt gcgggcgcct tcttcaagga tcgtcttgcg ttgtaaggct gggacaccgc
     6001 ggatactctt caccacgtcg tcaatggttg cggagtggcc aaagtcggcg cttacgatcc
     6061 aagcacagag gctgatttga gccgctaata gaattccctt taagctcatg agtttcccct
     6121 tcgtgatcgc ctcgtctaac accgcgacga cgtccggtca agctaatgtg aaatggcgta
     6181 agcccgccgc atcattacat ttaattgact tgcagaagtc gatttgctag taaacctgga
     6241 agccgatttg aggaggaaga gatgacctat gttattgccg aaccctgcat agacaccaag
     6301 g
//
LOCUS       DASULZ010000044         2573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_613717, whole genome shotgun sequence.
ACCESSION   DASULZ010000044 DASULZ010000000
VERSION     DASULZ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613717"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2157)
                     /locus_tag="VFY96_00865"
     CDS             complement(<1..2157)
                     /locus_tag="VFY96_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011144016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5605033.1"
                     /translation="MNISGLFIRRPVTTTLVMVGILIFGILGYQSLAVSDLPTVDFPT
                     ITVSANLSGASAETMASSVATPLEKNFSTIAGLDSMSSTSTLGRTQITLQFSLSRNID
                     AAAQDVQSMIARTARDLPPSMTSPPSYRKVNPANNSILLLALTSDTLPLSTVDEFAQT
                     ILAQRISMVSGVAQVDVFGSQKYAVRVQLDPNQMGVRQIGLDDVVAAIQRSNVDLPVG
                     TLNAPTRAYTFVSNGQLLNAAAFQSMVVTYRNGAPVRIQDIGKAIDSVENNQVASWFK
                     DKKAVILGVQRQPGANVVGVVDAIKEILPGLRAQIPQSVKLDVFFDRTESIRASVHDV
                     QLTLVGTIILVVLVIFLFLRNVSATIIPSLALPMSIVGTFAVMYLLDYSLDNLSLMAL
                     TLAVGFVVDDAIVVLENIVRHMEEGEKPYEAAVKGALEIGFTIVSMTISLVAAFIPIL
                     FMSGMLGRLLREFAVTICVSILVSGFISLTLTPMLCSRYLRPPADEKHGRLYLFLGRC
                     LDLMNKGYEITLAWVMRHHVTTMLASLVVLAATVHMFGLVPKGFIPSEDTGQILINTE
                     GAQGVSFEQMSRNQQQVADIVLQDPNVEAFFSSVGIGGVGLSGNTGRIFMKLKPRSER
                     PLVCSTFLGVKSCRSLSSEELIREMRPKLARVPGIRVSLQNPPVIRVGGRLSRSLYQF
                     TLQSPDAPELYRNAALFEDRLRALPDFRDVSSDLQLK"
     gene            complement(2154..>2573)
                     /locus_tag="VFY96_00870"
     CDS             complement(2154..>2573)
                     /locus_tag="VFY96_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5605034.1"
                     /translation="TTGTIKLKAEFKNSDLRLWPGQYTNVALTLSTQQGAVVVPSDAI
                     QVGPEGQQVFVVKEDKRVEVRPVTVGQSQEGESIITKGLAPGEVLVREGQFLLGPNSR
                     IEIKTAGADTSNSGGQGRKGQGERGKTKSQSDQRGAS"
BASE COUNT          628 a          740 c          679 g          526 t
ORIGIN      
        1 tttaagctgg agatcgctgc tgacatcgcg aaaatccggc agcgcgcgca agcggtcttc
       61 aaacaacgcc gcatttcgat aaagctcggg cgcatcaggg ctctgcaaag taaattgata
      121 gaggctacgg ctaagccgcc cgccaacccg gatgacgggc gggttctgca aagaaactcg
      181 gattcccggc acgcgtgcca gtttagggcg catttctcgg atcaactcct ccgagctgag
      241 cgagcggcaa gattttactc caaggaacgt agaacaaacg agcggccgct ccgatcgcgg
      301 tttgagcttc atgaagattc ggcccgtatt tcccgataat cccaccccgc cgattccgac
      361 actggagaaa aatgcttcta cattcggatc ttggagcaca atatcagcga cctgctgctg
      421 attgcgcgac atctgttcaa acgaaacccc ttgcgcgccc tcggtattaa ttaaaatctg
      481 accggtatct tcgctcggaa taaatccctt cggtaccagt ccgaacatgt gcactgtcgc
      541 cgcgagaacg actagcgatg ccagcatcgt cgtaacgtga tgccgcatta cccatgcgag
      601 agtgatctca taacctttgt tcatgagatc caggcaacgg cccaggaaca ggtacaaccg
      661 tccgtgtttc tcgtccgcag gcggacgcaa atagcggctg cacagcatcg gcgtaagagt
      721 taacgaaata aatcccgata ccaaaatcga cacacagatc gtcaccgcaa actcgcggag
      781 cagccgtccg agcatgccgc tcatgaagag gatcgggatg aaagcggcga ccagcgaaat
      841 cgtcatggaa acaattgtaa acccgatctc tagagctccc ttgacggcgg cttcataggg
      901 tttctcgccc tcttccatat gtcgaacaat gttttccagg accacgattg cgtcgtcaac
      961 gacaaagccg acagcgagag tcaaagccat caacgacagg ttatcgaggc tatagtccag
     1021 caaatacatg acggcaaaag tgccaacgat cgacatcggc agagccaaac tcggaatgat
     1081 cgtcgcggac acgttgcgta ggaacaagaa aataaccaac acgacgagta taatcgtacc
     1141 caccagagtc agctgaacgt cgtgcacgga agcgcgaatc gattcggtgc gatcgaaaaa
     1201 tacatccagc ttaaccgact gcgggatctg cgcgcgcaac cccggaagaa tctctttgat
     1261 cgcgtcgaca accccgacca cattcgcccc gggctgccgc tgcacgccga gaatgacggc
     1321 cttcttgtct ttgaaccagc tcgcaacctg attgttttcg accgagtcga tcgctttgcc
     1381 gatatcttgg atccgcaccg gcgcgccgtt acgataggtc acgaccatgg actgaaatgc
     1441 ggccgcgttg aggagctgac cgttggaaac gaaagtgtac gcgcgggtcg gtgcgttcag
     1501 cgtgccgacc ggcaaatcga cattactgcg ctggatcgcc gccacgacat cgtcgagacc
     1561 gatttgtctg acacccatct gatttgggtc gagctgcacc cggaccgcgt acttctgcga
     1621 accaaagaca tcgacctgag cgaccccgct caccatcgaa atacgctgcg ccagaatcgt
     1681 ctgagcaaac tcatcaaccg tcgacaaggg aagcgtatct gacgttaacg ccaacagcag
     1741 aatcgaatta ttggcagggt tgactttgcg gtaggaaggc ggactcgtca tgctcggcgg
     1801 caagtcacgc gccgtccgag cgatcattga ctgcacgtcc tgggcggcgg catcgatgtt
     1861 acggctcaaa ctaaattgaa gggtaatttg cgtgcgacca agtgtgctcg tcgagctcat
     1921 cgaatcaagg ccggcgatcg tggagaagtt tttttctaac ggagtcgcca cggatgaagc
     1981 catggtctcg gcgctggcgc ccgacaaatt ggcgctgacg gtaatcgtgg gaaaatccac
     2041 cgtcggcaaa tcgctgaccg cgagggattg gtaaccgagg ataccgaaga tcaggatccc
     2101 gaccatcacc agagtggttg taaccggacg ccgaatgaaa agtccggaga tgttcacgat
     2161 gcacctcgtt gatcgctctg gctcttggtc ttgccgcgtt ctccttgacc ctttctgccc
     2221 tgcccaccgc tattggacgt atcggcgccc gcggtcttaa tctcaatccg agagttagga
     2281 ccaagtaaaa attgtccttc acggacgagg acctcgcccg gcgcaagacc tttggtaatg
     2341 atcgattcac cttcctgact ttggcccacg gtgaccggcc ggacttcgac tcgtttgtcc
     2401 tctttcacga caaaaacttg ttgcccttca gggccgacct gaatcgcatc cgaaggaacc
     2461 acaacggcgc cttgctgcgt cgagagcgtc aaggcaacgt tggtatactg gcccggccaa
     2521 agtcgtagat ccgaattctt aaactcggcc ttgagtttga tcgtgcccgt agt
//
LOCUS       DASULZ010000045         3150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1019729, whole genome shotgun sequence.
ACCESSION   DASULZ010000045 DASULZ010000000
VERSION     DASULZ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019729"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /gene="fmt"
                     /locus_tag="VFY96_00875"
     CDS             <1..851
                     /gene="fmt"
                     /locus_tag="VFY96_00875"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX5605035.1"
                     /translation="VGVVTQPDRPSGRGQRPVRSPVRKLAEAHGVPVIAPEKIRTPEV
                     LETLRDWRPQIIVVVAYGRILPKTILDLPPYGCLNVHYSLLPKYRGAAPAAWTIINGE
                     EQGGVTTMKLVEKMDAGPIYLQEPVPLAPDETTGSLQRKLTPVGGRLLAQTLRRLKEG
                     ALIPREQDESRASLAPMLKKQDGLIEWRRAAVEIERRVRGLNPWPGSFTYLNGRLLKV
                     HRTMIITENRGEPGEIIRADSGGFWVATAAATLSLEEVQLENKKRLPGSEFIKGARTR
                     AGDRLG"
     gene            844..2199
                     /gene="rsmB"
                     /locus_tag="VFY96_00880"
     CDS             844..2199
                     /gene="rsmB"
                     /locus_tag="VFY96_00880"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEX5605036.1"
                     /translation="MAEKARSLRAIAAEILSQVDTRKAYADILLDRALKNSCMDERDR
                     ALLTEVVYGTLRWRGKIDAALVKYLHQPFSRTDQRIRNLLRLTCYQLLFLDRVPDYAA
                     VSEAVEIAKLHGGKRAAGFVNGVLRNFLRSKPGNTIAPENPSSSSLAVEYSHPEWLVK
                     LWTEQFGLESAIGLMRANNEKSPLVLRANSLKCSRDQLLQLLLDAGIKATPTRQSPHG
                     ISIQSTGAVEKLSGFAEGLFQVQAESSQLIAPLLSALPGERILDACAAPGGKATHIAE
                     TIKDRGEIVAIDKSATGIARLRENLTRLGITAVRPYRADVSQKLTDELSGPYDRILVD
                     APCSGFGTLRSHPEIKWHRDLANVRRLRALQSKILDCVAPSLKLGGIFVYATCTLTAE
                     ENERNVESFLARHPGFELENAARYLPDEAKQMVKANYFEALPNRDNTDGFFAARMKRI
                     S"
     gene            2200..2865
                     /gene="rpe"
                     /locus_tag="VFY96_00885"
     CDS             2200..2865
                     /gene="rpe"
                     /locus_tag="VFY96_00885"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX5605037.1"
                     /translation="MASVKIAPSILSADFSRLKDEIAEVEAAGADWLHVDVMDGHFVP
                     NITIGPVVVESVRKVTKIPLDVHLMITDPDKYAPEFIKAGADWVSVHPDTCPNPNATL
                     ERIRELGAKCSVAVNPDVPLDKVELCFDHIDMILMMTVFPGFGGQAFIPDVLTKIAAV
                     KKIFDQRPAEILIEVDGGIKADNIARVVAAGGEVIVSGSGIFKTPDYAATIRQMRRAV
                     SAS"
     gene            2942..3018
                     /locus_tag="VFY96_00890"
     tRNA            2942..3018
                     /locus_tag="VFY96_00890"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2976..2978,aa:Pro,seq:cgg)
BASE COUNT          708 a          890 c          867 g          685 t
ORIGIN      
        1 ttgtcggcgt cgttacccag cccgatcgtc cgtccggtcg cggccaacgg ccagtgcgat
       61 caccggtccg caagctggcc gaagcgcatg gcgttccggt gatcgcgccg gaaaagatca
      121 ggacgccgga agtgctcgaa accttacgcg actggcgccc acagattatc gtggtcgtcg
      181 cctacggacg aattctcccg aaaaccattt tggatctgcc gccatacggc tgtctcaacg
      241 ttcactactc gctcttgccg aaatatcgcg gtgcggcccc agcggcttgg accatcatca
      301 acggagaaga gcagggcggc gtgacgacca tgaaactggt agagaagatg gatgctgggc
      361 cgatttacct ccaggaaccc gttccactcg ctcctgacga aaccaccggt tcacttcaaa
      421 gaaagctcac tccagtcgga ggccgcttgc tcgcacaaac tctccggcgg ctcaaagaag
      481 gagctctcat tccgagagaa caagacgaat ctcgggcatc attggcgccg atgctgaaga
      541 aacaggatgg actcatcgag tggcgaagag ctgccgttga aatcgagcgg cgtgtgcgcg
      601 gtctcaatcc ttggccgggg agtttcactt atttgaacgg acgcctactc aaggtgcacc
      661 ggaccatgat catcactgag aatcgcggcg agcccggcga aattattcgc gcggatagcg
      721 gcgggttttg ggtggcaaca gccgccgcga cccttagcct cgaagaggtt caactggaaa
      781 acaagaaaag gctgccgggc tcagaattca tcaagggtgc gcgaactaga gccggtgatc
      841 gccttggctg aaaaagcccg cagcctacgc gcaatcgccg ccgagattct ttcgcaagtg
      901 gacacgcgca aagcttatgc cgacattttg ctcgatcgag cgctcaagaa tagttgcatg
      961 gatgagcgcg accgggcgct gctcaccgaa gttgtttatg gcactctccg ctggcgcggc
     1021 aaaattgacg cagccttggt taaatatctg catcagcctt tttcccgcac ggatcaaagg
     1081 atccgcaatt tactgcgcct cacctgttat cagctgctct ttctcgatcg cgtccccgat
     1141 tacgccgcgg tgagcgaagc tgtggaaata gccaagctcc acggcggcaa aagagctgcc
     1201 ggtttcgtta atggcgtgct gcgcaacttt cttcggagca aaccgggtaa cacgatcgcg
     1261 ccagaaaacc cgtcgagctc ctccctcgcc gtcgaatatt cccatcccga gtggctggta
     1321 aaactgtgga cggaacaatt cggtcttgaa tccgcgatag gattaatgcg cgccaacaat
     1381 gaaaaatcgc ccttggtctt gcgcgctaac tcgctcaaat gtagcagaga ccagttgttg
     1441 cagctgctgt tagacgccgg aattaaagca acgccgaccc gacaatcacc gcacggaatt
     1501 tctattcaat cgactggcgc tgttgaaaag ctttccggtt tcgctgaagg attgtttcag
     1561 gtgcaagcgg agtcgtcgca gcttatcgcg ccgctgctct cggcgctgcc aggagaacgg
     1621 atccttgacg cctgtgccgc gcccggcggt aaagcgacac acattgccga aaccataaaa
     1681 gacagaggcg aaatcgtcgc catcgacaaa tccgctacag gtatcgccag actgcgtgag
     1741 aaccttaccc gtttgggcat caccgccgtt cggccgtatc gagctgacgt gagccaaaaa
     1801 ctcactgatg agcttagcgg gccctacgac cgcattctcg tggacgcacc ctgcagcggc
     1861 ttcggcaccc tgcgctcgca tcccgaaatc aagtggcatc gggatctcgc caatgttcgc
     1921 cggctccgcg cgttgcagtc aaaaatactc gactgcgttg ctccctctct aaagctcggc
     1981 gggattttcg tctacgcgac ctgcacgctg actgcggagg aaaatgagcg caacgtggaa
     2041 tcctttctcg ctcgtcaccc cgggtttgag ttggaaaatg ccgcgcgcta tcttcccgac
     2101 gaggccaaac agatggtcaa ggcgaactac ttcgaggcgc tgccgaaccg cgataatacg
     2161 gacggatttt ttgccgcgcg catgaagagg atttcctgaa tggcatcggt aaaaatcgcg
     2221 ccatcgatac tctccgcgga ctttagccgg ctcaaggacg aaattgccga ggttgaagca
     2281 gcgggcgcag attggctgca cgttgatgtc atggacggcc acttcgtacc gaatatcacc
     2341 atcggccccg tggtcgttga atccgtgcgc aaggtcacca agattccgct ggacgttcat
     2401 ctgatgatta ccgatccgga taaatacgcg ccggaattta tcaaagccgg tgccgattgg
     2461 gtatcggttc atcccgacac ttgtccgaat ccgaacgcta ccctggagcg cattcgggaa
     2521 ctgggcgcga agtgctccgt cgctgtcaat ccggatgtgc cgctggataa agttgaactc
     2581 tgcttcgacc acattgatat gattttaatg atgacggtat ttcccggttt tggcggccag
     2641 gcctttattc ccgacgttct caccaaaatc gccgcggtta aaaaaatctt tgatcaacgc
     2701 ccggcagaaa ttctgatcga agtcgatggt ggtatcaaag ccgataatat tgctcgggtt
     2761 gtcgctgccg gtggagaagt aatcgtgtcc ggttctggaa tattcaagac cccggattac
     2821 gctgctacca tccgccagat gcgccgtgcg gttagcgcca gttagcgtcg attcggttct
     2881 aaggggaagg agcgcgcttt agctgttgtt ttctggtttc ggtgtgttag tttgagccga
     2941 tcggggcgtg gctcagcttg gtagagcgca cggttcggga ccgtgaagtc ggtggttcaa
     3001 atccactcgc cccgaccatt tcctaatttc taaattccaa caacaatttc ttttcccgag
     3061 aaccgcgaaa gtaacgcagc gaatggttaa ttctcacgtt tcgcgttcag gatatatcga
     3121 accagcatgg tagcacggtc accggtttgc
//
LOCUS       DASULZ010000046         5353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_918449, whole genome shotgun sequence.
ACCESSION   DASULZ010000046 DASULZ010000000
VERSION     DASULZ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918449"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..310)
                     /locus_tag="VFY96_00895"
     CDS             complement(107..310)
                     /locus_tag="VFY96_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605038.1"
                     /translation="MRYFGPLALLFVLSASFVGCTATHDRVIYQRYAGHNYCHTKVET
                     AGDPLMPTERDVVDYYGPCDAFD"
     gene            complement(570..1184)
                     /locus_tag="VFY96_00900"
     CDS             complement(570..1184)
                     /locus_tag="VFY96_00900"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX5605039.1"
                     /translation="MNNRSLMFFLWITFSAATAWTAEEGPNRPEPKPVTVDSKSTAVL
                     VLDLNTRCDDPKQVCSKITASVGDFLDKARAASVPIIYTVSTAAKGKPIGELAAPLRR
                     KENEVIIYPDGFDKFYSGELQPLLKDKDVKTLIITGSSTNSAVLYTATTAARMHRYTV
                     VIPMDGVNAATPYEQEYTIHQFTVLPSEANKLFHFTNLSMIRFR"
     gene            complement(1239..2384)
                     /locus_tag="VFY96_00905"
     CDS             complement(1239..2384)
                     /locus_tag="VFY96_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605040.1"
                     /translation="MTPPSVQDIDRIAELRDPVVRNLQITQCYHEISQCVAERTGFCA
                     NWCTFATWASRQAGQTIRQEDLIRAFEERFFLSPEISATLEDINAKLTQLGARLQAGF
                     SAKEIFQAFNPAAAFARASAAVARGNKKVFEEIGFEFARFLQTVRVGNRFDRDQTARF
                     CAALRPGDPPDGQRLLREAFAAYSEASVQDGKQKAELMLLANIAAGYHEQIRLQPEIA
                     EALNASVAGAMDFKRSLLTRLVAHFHLREDLLTRRLPHLDALFGRLSGEVNRLIRQVI
                     TDHLMTLHLSSTAVLRLGRDLSGEFPQSLVRLHNPRLAALLAEVDLTPESLNATGATD
                     WANFQQRMHFIADFFRVYQERQYLFETPFSAPQIELLKMGRRPDGPL"
     gene            complement(2566..3435)
                     /locus_tag="VFY96_00910"
     CDS             complement(2566..3435)
                     /locus_tag="VFY96_00910"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605041.1"
                     /translation="MIAKDAGLFREQDLDVDLVFMGSSNLIIQSMLAGSVPVAGVAGP
                     AVISSVVGGGDVVTVANLAPLTIALMVKPSIEKPEDLRGKKIGVPRLGAVAHFAIRMI
                     LDRHGIKDAAILQMGSQPEAAAGMRRQAIDGAMISLPLNYVLEKEGYRQLVGPQDYRK
                     LGIQFISQGISARKSYGAKNRDVVLRLIKATMQGLKLMSTDEPLAKKTLAKYTRQTDP
                     ESLDRAYRFGLETLNKDPTIPQEAIVSMVRLMADLALIDRSAASNTPPEAFYDNSFVD
                     EMKRTGFLADLWK"
     gene            complement(3711..4877)
                     /locus_tag="VFY96_00915"
     CDS             complement(3711..4877)
                     /locus_tag="VFY96_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEX5605042.1"
                     /translation="MNQSVERILTTHVGSLPRPADLLQMNDVRANGRDFDAAIWAARV
                     KSAIAEVVRLQRDAGIDVVNDGELSKSSWSSYVNDRLGGFAESVSRGQGSLARGRDRK
                     AFQEFYDEYDRIQPFRTVNGSRWTDVVCNAAVTYTGHNAVQMDIENLKAACGSEEVKE
                     AFITAVAPGSIVTRRINRFYPSEEAFLFAIAEAMKTEYQAIVDAGFLLQIDDPQLVTR
                     YDAEDPPPDAEQYRKRAGVRVEALNCALAEIPPERIRYHICWGGWHGPHTTDLPLKDI
                     VGLLLQIRAGAYLIEAANARHEHEWRVWEEVKLPPGKILIPGVVSHATNVIEHPELVA
                     ERIARFANLVGRDNVIAGTDCGLGGRLHHQLVWAKLKALAEGARLASKDLWGRM"
     gene            5168..>5353
                     /locus_tag="VFY96_00920"
     CDS             5168..>5353
                     /locus_tag="VFY96_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605043.1"
                     /translation="MSALLFVCSILSTGAMAQTRRTPERNRITVGLVAEANQQEVEKH
                     VQEFVEYLARKLSSDGKT"
BASE COUNT         1193 a         1470 c         1477 g         1213 t
ORIGIN      
        1 aaggaatagg atcactgagc ccgcgctgtg tcaggcggta tctcaataga aaacccaacc
       61 gcgaattcga catcctgaat gcgaatagaa gtgccccgac tgctcatcaa tcaaacgcat
      121 cacacgggcc gtaataatcg accacgtctc gttcggtcgg catgagcgga tcgccggcgg
      181 tctccacctt tgtatggcaa taattatgac cggcgtagcg ctgatatatg acgcgatcgt
      241 gagtcgcggt gcatccaacg aacgaagcgg ataagacgaa cagcaatgct aatggtccaa
      301 aatacctcat tgttgatctc ctcctggctg cgtagagaat caagatatgt gccatttgcc
      361 ttatcgtgcc atagctggtc gtaagcgcgt agtccggtta gatattaata agataccagc
      421 gaccaaaggc ctgattcggc caggttccaa catcaaccag agaattgtct atcccgctgg
      481 tagagaaatt cggacataca catcgaccga acggatgaga tctgacggct tgaacagctc
      541 gaacgattaa acggcttgca ccctcagtgt cagcgaaagc gaatcatcga caggtttgtg
      601 aaatggaaca atttgttcgc ttctgaaggc agaactgtaa attggtgaat ggtatattcc
      661 tgttcatatg gcgttgccgc gttcacaccg tccattggaa tcaccactgt atagcgatgc
      721 atgcgcgccg ctgtcgtcgc ggtgtagagc acggctgagt tcgtcgacga cccggtaata
      781 atcaaagttt tcacatcttt gtctttaaga agaggctgga gctcgccact gtaaaatttg
      841 tcgaacccat caggataaat gatcacctcg ttttctttcc gcctgagggg tgccgccagc
      901 tcgccgatcg gctttccctt ggccgcagtt gataccgtat aaataatcgg cacggatgca
      961 gccctggctt tgtcgaggaa atctcccaca gaagcagtta ttttcgaaca aacttgtttg
     1021 ggatcgtcgc agcgggtatt gaggtcaagc accaacacgg cggtgctctt gctgtccaca
     1081 gttactggtt tcggctcggg ccggttcgga ccctcctctg ccgtccatgc ggtcgcggca
     1141 gagaatgtga tccaaagaaa aaacatcagc gatcgattgt tcatttcaat tctcctgtct
     1201 acggtgctaa ctcgacacgc tatttcacga ccaagttttt acagtgggcc gtcgggtcgc
     1261 cggcccatct tcaatagctc gatttgcggg gcgctaaatg gcgtctcgaa cagatactga
     1321 cgttcctgat aaacacggaa aaaatcggcg atgaagtgca tccgctgttg gaaattagcc
     1381 caatccgtcg caccggttgc gtttaaactt tcaggcgtca aatcgacctc ggcgaggagc
     1441 gctgcgaggc gcgggttatg gagccgcacc agtgattgcg gaaattctcc gctcagatca
     1501 cgtccgaggc gaagcaccgc cgtgctcgac aagtgaagcg tcattaaatg atcggtgatc
     1561 acttgtcgaa tcaggcggtt cacttctccc gacaacctgc cgaacagagc gtcgaggtgg
     1621 ggcaggcgcc gcgttagaag atcctcgcgc agatgaaaat gcgcgaccag ccgcgtcaac
     1681 aggctgcgtt tgaaatccat cgctccggcg acagatgcgt taagcgcttc ggcgatttcg
     1741 ggttgaagcc gaatctgctc gtgataacca gcggcaatgt tcgctaggag catcagctcg
     1801 gctttctgct tgccgtcctg aactgacgcc tcggagtagg ccgcgaacgc ttcgcgcagc
     1861 agtcgctgtc cgtccggtgg gtctccggga cgaagcgccg cacagaagcg cgccgtctgg
     1921 tcgcgatcga agcggtttcc gacgcgaacc gtttgcaaaa accgagcgaa ctcaaaaccg
     1981 atttcttcga agactttctt gttaccacgc gcgacggcgg cgctcgcgcg agcaaatgcc
     2041 gcggcggggt tgaacgcctg aaaaatttct ttcgccgaaa aaccagcctg taatctggcg
     2101 ccgagttgcg tcagtttggc attgatgtct tcgagagttg cggagatctc cggtgaaaga
     2161 aaaaaacgtt cctcaaacgc gcggatcaga tcttcctgcc ggatggtttg gcccgcttgg
     2221 cgcgatgccc aagttgcaaa cgtgcaccaa ttagcgcaaa aaccagtgcg ttcggcgaca
     2281 cactgggaga tctcgtggta gcattgcgtg atctgaaggt tacgcacgac tggatcgcgc
     2341 agttcggcga tccggtcgat gtcttgaacg ctcggcggcg tcatatcaga cgtgccacag
     2401 cagcggtcat atttatcgat ttgaccgctg aattcagcac gatgcttgtt gccgagcaaa
     2461 atggtgagaa cgaagggatt tcacagcggt catcttgcca tcgctttgga gttgatggcc
     2521 gggaaaactt cggctgatag tgacggccgc ggtacactga cgatctcact tccataaatc
     2581 ggccaaaaat ccggtccgct tcatttcatc gacgaagctg ttgtcataga acgcttcggg
     2641 cggtgtgttg cttgctgctg agcggtcgat gagagcaagg tcggccatca gacgcaccat
     2701 cgatacgatc gcttcctgcg ggatcgttgg gtccttgttc agcgtctcaa gtccgaagcg
     2761 ataggcacgg tcgagggact ccggatctgt ttggcgcgtg tattttgcta gagttttctt
     2821 tgccagaggt tcgtcggtcg acatcagctt taagccctgc attgtcgctt tgatgagtct
     2881 caggacaacg tcgcggttct tcgcgccata ggatttgcgc gcggagatgc cttgggaaat
     2941 aaactgtatg ccgagcttac ggtagtcctg aggaccgacg agttggcgat acccttcttt
     3001 ttcgagcacg taatttagcg gcagcgaaat cattgcgccg tcgatcgcct gccgtcgcat
     3061 acctgcggcg gcctcaggtt ggctgcccat ttgcagaatc gcagcatctt ttattccgtg
     3121 gcggtcgaga atcatgcgaa ttgcgaagtg cgcgaccgct cccaagcgcg gcacgccgat
     3181 cttctttccc ctaagatcct cgggtttttc aatggagggc tttaccatca gcgcgatcgt
     3241 tagcggcgcg agattcgcga cggtcacaac atcgccccca ccgacgacgc tcgatatcac
     3301 cgccgggcct gccacgccgg ccacggggac cgacccggca agcatcgatt ggatgatcaa
     3361 gttcgaggag cccatgaaaa cgagatcgac gtcaaggtcc tgctcgcgga agagcccggc
     3421 atctttggca atcatccagg gcaggctgct gtagttgacc accgagtaag gaaagagtat
     3481 tttttgcagc ttctgcgcat aaccgtcggt tatgccgatt atctgaagga tagcgagtgc
     3541 aaataatgac ttttttcccc tcgcaacgaa ttttgctgcc caactaaacc ctggtgagcg
     3601 cgaggggttg gagattctac cgcagagacg cgcaggacac tgagataggc gtcgcgtctg
     3661 aagacatatt cggacccctg caaactctct gtcgccgtgg cgaagttccg tcacatccgc
     3721 ccccagagat ccttgcttgc caagcgcgcg ccttccgcta atgccttgag cttcgcccag
     3781 acgagctggt gatgaagacg cccgccgagg ccgcaatcag taccagcgat aacgttatct
     3841 cgacccacca gattagcgaa acgcgcgatg cgttcggcca ccagctctgg atgctcgatg
     3901 acgttggtgg cgtgactgac aacaccgggg attaaaattt ttccgggcgg cagcttcact
     3961 tcttcccaca cgcgccactc gtgttcgtga cgcgcattgg cagcctcgat caaataggcg
     4021 ccggcgcgga tttgtaagag tagaccgaca atatccttga gcggcaaatc ggtcgtatgt
     4081 ggtccatgcc agccacccca gcaaatatga taacgaatgc gctctggcgg aatttccgcg
     4141 agtgcgcaat tgagcgcctc gacccgcacc cccgcccgct tgcggtattg ttcagcgtcg
     4201 ggaggcggat cttccgcgtc gtagcgagtg acgagctgag ggtcgtcgat ctgcagcaaa
     4261 aagccggcat cgacgatcgc ttggtattcc gtcttcatag cttcagcaat ggcgaacaga
     4321 aaagcttcct cgctcgggta aaagcggttg attcgacgag tgacgatgga gcccggagcg
     4381 actgccgtga tgaatgcctc cttcacttcc tccgagccac acgctgcctt caagttctct
     4441 atgtccatct ggacggcgtt atgtcccgtg tatgtgaccg ccgcgttgca aaccacatcc
     4501 gtccaacgcg agccgttgac cgttctaaaa ggttgaattc tgtcgtactc gtcataaaac
     4561 tcttgaaacg cttttctgtc tcggccgcgc gccagcgacc cttggccgcg agaaacactt
     4621 tcggcgaatc cgcctagacg gtcattgacg tagctcgacc agctcgactt cgacaattcg
     4681 ccatcgttca cgacatcaat gccggcgtcg cgctgcagcc ggacaacttc ggcgattgcg
     4741 ctctttacgc gcgctgccca gattgcagcg tcgaaatccc tcccatttgc ccttacgtca
     4801 ttcatctgca aaagatcggc gggccgggga aggctgccca cgtgagtggt gagaattctc
     4861 tcaacgcttt gattcaccga tgattctcct ttgcttctcg ctgggcaatc tttatatctt
     4921 ctcgcgttta ttggaaacag ttcagcgccc atcggtcgat gggacatagg cgcgctggtt
     4981 cgcatgcgtg gacaactggg cgacggacta gtgcaggaag cgaacgggtc gtaaaaggca
     5041 tcgagccagg catcgtaatc gactggcaca gagcggcccg ctcggtcgtg cttgccaagg
     5101 agaggtataa tacctacgcc acaggacgca cgatgccttc aacaaagctt acctacggct
     5161 cgtagtcgtg tctgcgctgc ttttcgtctg cagcattctg agcaccgggg cgatggctca
     5221 aacgcggcgg acacccgaaa gaaatagaat tactgtggga ctggttgccg aggcaaatca
     5281 gcaggaggtg gagaagcatg ttcaggagtt tgtcgagtac ctggcgagaa aactttcctc
     5341 tgatggaaag act
//
LOCUS       DASULZ010000047         2979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1222881, whole genome shotgun sequence.
ACCESSION   DASULZ010000047 DASULZ010000000
VERSION     DASULZ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222881"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="VFY96_00925"
     CDS             <1..1128
                     /locus_tag="VFY96_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX5605044.1"
                     /translation="YIREAYGKLMGFLSGWSSFLVTFSGAIAFLAVGFTGFLSFFIPA
                     LGSNEPFFSVNGFGLSIHPTGGTLFSIIVVLSISALHCLGVRQGTVTQNILTTLKLGA
                     LLAIILLGISIGSGDRAHFTPLFDWSKISNFSVFAAAFIPAIFAYSGWNAVIYIAGEV
                     KDPERNLPKALLWANLLVITLYLAINMVYIYGVPVTEMKGALRVSEVATTALFGYQTS
                     ALITAIITVSILGALNVVTMLGPRIYYAMARDGVFFRRLTYIHPTFGTPTSAIILQAV
                     WTCFLILTNTWGTLFTYVSVVITLFSALTVGSVIVLRYKRPELKRPYKLWGYPVVPIL
                     FIIANAWIVWGSVTEKPYESLIGLLIVCLGIPAYLIWQKKL"
     gene            1193..1807
                     /locus_tag="VFY96_00930"
     CDS             1193..1807
                     /locus_tag="VFY96_00930"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX5605045.1"
                     /translation="MIRITKVYTRTGDKGDTALVGGKRVAKDSPRIEAYGTIDELNSV
                     VGLARVFNAESLDAGDAHRFLNEVLCQLQDELFDLGGELATPTESFQEGMYRVGSEQV
                     DRLEKWIDQCQQDLEPLKSFILPGGGRIGGYLHHCRTVCRRAEREILRLSRSEEINRH
                     VLEYFNRLSDLFFVLSRWIAKQTGEPEYLWQRGLMEKPKNKKAK"
     gene            1997..2767
                     /gene="fabI"
                     /locus_tag="VFY96_00935"
     CDS             1997..2767
                     /gene="fabI"
                     /locus_tag="VFY96_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEX5605046.1"
                     /translation="MKLLEGKKAAIFGVANERSIAWAIAEAFHAQGAELAFTYAGEML
                     EKRVRPLAEGVGSKIILPCDVTKDEEVEKVFAYLREAWGGLDILIHAIAFANKEDLAN
                     PYVQTSRAGFHLAMDVSAYSLIALAQRAAPLMEGRGGAMVTLTYMGSERVVPNYNVMG
                     VAKAALEASVRYLAHDLGPKGIRVNAISAGPIRTLAAAGISDFKTMLHHVAERAPLKR
                     NIDAEEVGKTALYLCSDWGSAVTGEVLHVDAGYNIMGM"
BASE COUNT          648 a          777 c          850 g          704 t
ORIGIN      
        1 tatatccgtg aggcgtacgg aaagctgatg ggcttcttat ccggttggtc atcgtttctc
       61 gtaacgtttt cgggcgcgat tgcgtttttg gcggtcggat ttaccgggtt tctttcgttt
      121 tttattcccg cgctcggttc gaatgagccg tttttttcgg tgaacggatt cggcttgtcc
      181 atccatccga ctggcggcac gttattttcg atcatcgtcg tgctctcgat ttccgcgctg
      241 cattgtctcg gcgtgcgcca gggcacggta acgcagaaca ttttaacgac tttgaaactc
      301 ggcgcgctcc tggcaattat cttgctggga atttctatag gctccggcga ccgggcgcat
      361 ttcacgccgc tattcgactg gagcaaaatt tcgaacttca gtgtttttgc cgcggcattt
      421 atcccggcaa tcttcgccta ctcgggttgg aacgcggtca tttatatcgc tggtgaagtc
      481 aaagacccgg agagaaatct gcccaaggca ctactctggg ccaatctcct ggtgataacg
      541 ctgtacctgg cgatcaacat ggtttacatc tacggcgtcc cggtgacaga gatgaaaggc
      601 gcgctgcgcg tctccgaggt cgccaccaca gcattgttcg ggtatcagac ttccgccttg
      661 atcacggcga tcatcacggt ctcaattctc ggcgcgctta acgtcgtgac gatgctcggc
      721 ccccggattt attatgccat ggcgcgcgac ggagtgtttt tccggcggct cacctatatt
      781 caccccacgt tcggcacgcc taccagcgcg atcattcttc aggcggtatg gacctgtttc
      841 ttgattttga ccaacacgtg gggaacgctc ttcacctacg tgagcgtggt gatcacgctg
      901 ttttctgcgc ttaccgtcgg ttcggtcatt gtgctccgct acaaacggcc tgaattgaag
      961 cgtccttaca aactgtgggg ctatccggtt gtgccgatct tgttcatcat tgccaatgcc
     1021 tggattgttt ggggttcggt gactgaaaaa ccctatgaat cattgatcgg cttactgatt
     1081 gtttgcctcg gcatcccggc ttatcttata tggcagaaaa agctataaag atgagcgaat
     1141 agcgcgagcc ttccggagag aatctcgtgt tcacagcgag gcaaaccagt tcatgatcag
     1201 aatcacgaaa gtttataccc gcacgggcga caagggcgac accgctttgg tgggcggcaa
     1261 gcgtgtagcc aaagattcgc cgcgcatcga agcctacggg acgatcgacg agctcaactc
     1321 ggtcgtcggt ctggcgcgcg ttttcaatgc cgaaagcctg gacgccggcg atgcgcaccg
     1381 gtttctcaat gaggtgttgt gccaacttca ggacgagctt ttcgatttgg gcggcgagct
     1441 ggcgacaccc acggagtcat ttcaggaagg catgtatcgc gtcggcagtg aacaagtcga
     1501 ccggctggaa aaatggatcg atcaatgcca gcaggatctc gaaccgctca aatcttttat
     1561 tctcccgggc ggcggtcgga tcggcggtta cttgcatcat tgccgaacgg tctgtcgtcg
     1621 ggccgagcgc gaaatcctgc ggctctcgcg tagcgaagag atcaaccggc acgtgctcga
     1681 atatttcaac cgcttgagcg atctgttttt tgttttgtcg cgctggattg ccaaacagac
     1741 cggcgagccg gagtatctgt ggcagcgcgg gctaatggaa aagcccaaaa acaagaaagc
     1801 caaatagttg agagtgcctc aatccgttat ggttcgacaa gctcaccatg cgcggtttag
     1861 gtattctgga agatcaagtg cttagccgtt catcctgagc atgtcgaagg acgaacggcg
     1921 agttttcacc cactctgagg aagtgagaga tctttcaaac tgcaacgatc aacttaagaa
     1981 tcgctgaggt cgtttgatga aactactcga gggaaaaaag gcggcgatct tcggtgtcgc
     2041 caatgagcgc agcatcgcct gggccattgc cgaggcgttc catgcgcagg gcgccgagct
     2101 ggcgttcacg tatgcgggag aaatgctgga gaaacgggtt cgtcctttgg ccgaaggcgt
     2161 cggttcgaaa atcattttgc cttgcgatgt aaccaaagac gaggaggtcg aaaaggtatt
     2221 tgcgtacctg cgggaggcct ggggcggact cgatatcctg atccatgcca tcgcctttgc
     2281 caataaagaa gacctggcga atccctacgt ccaaaccagc cgcgcaggat ttcatctggc
     2341 catggacgtg agcgcctact ctctgatcgc gctcgcgcag cgcgccgcgc ctttgatgga
     2401 aggacgaggt ggcgcgatgg tgacgttaac ttacatggga tcggagagag tcgttcccaa
     2461 ctacaacgtc atgggagttg ccaaagccgc gttggaagcg agcgtgcgat atctggcgca
     2521 cgatctcgga ccgaagggca ttcgggtcaa cgcgatttct gccggaccga ttcgaacctt
     2581 ggcggcggcc ggtatttccg atttcaagac gatgcttcat cacgtcgccg agcgcgcgcc
     2641 gctcaagcgt aacatcgacg ccgaggaagt tggcaagacc gcgctttatc tctgtagcga
     2701 ctggggcagc gcggtgaccg gtgaagttct gcacgtcgat gccgggtaca atatcatggg
     2761 catgtagcaa ttgcgctcaa tcaggtacag gagaaccatt atgagagaat gggaagtatt
     2821 cgtaccggag gaagaacgaa agatctatga gaaagccggc tacaagggca aagaaaaata
     2881 tggagtcaat ccggcgctct tgatcattga cgtcatcacc ggattcaccg gcaccaaacc
     2941 gatgccggtg atggacgcca tcgacgactt tcccaccag
//
LOCUS       DASULZ010000048         2358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_715667, whole genome shotgun sequence.
ACCESSION   DASULZ010000048 DASULZ010000000
VERSION     DASULZ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715667"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1306)
                     /locus_tag="VFY96_00940"
     CDS             complement(137..1306)
                     /locus_tag="VFY96_00940"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEX5605047.1"
                     /translation="MLLFALLLLPAPALAGAKEKVAALAPSATVFVVDADGKELVAQN
                     ADKPFVPASVTKIVTAWLVLEVLGEDYRFETKFYLDDRRVLYVRGGGDPFLVSEELAG
                     LAQGLVAAAGKQPITGMVLDASYYPSDLRIPGIEDTRRSYDALNSALAVNFNTIAAVR
                     KGNNVISGETQTPITPLAISQFRARGPKGSGRISLAQDPAVSLRYAGELIAAFIERAG
                     GSVKGKIATGTVPAGLTPVYVHRQSRSLPEILHELLVGSNNYIANQLFLEIGGHRLGG
                     PVNLKKSLKVANEMLAAHDLAGAIYLEEGSGISRGNRFTARGLAKVLSLFAPHAGLLK
                     KDKSASYKTGTLDGVRTLAGYATTSKNGRVRFAISLTGNDGELRFRLLKAIEPEL"
     gene            1526..1993
                     /locus_tag="VFY96_00945"
     CDS             1526..1993
                     /locus_tag="VFY96_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605048.1"
                     /translation="MRENLDIDTKTLALYYAWDHFAYHAQQRQTVFNFFLLVVGGCVA
                     AYSAMIGESGTEYSYFRSFLGAVLALASLLFWRLDQRNARLVNVSEEALKKLEARMAD
                     EIKEPSIRLIENADKKSARFLFSKIESFSQIYGVVFAVSGLVGVAMATLSVRG"
     gene            2120..2314
                     /locus_tag="VFY96_00950"
     CDS             2120..2314
                     /locus_tag="VFY96_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605049.1"
                     /translation="MNTITNETAEVSSGTYFAHLFKLDADQYQLLQKPRFHRDCDYLN
                     RPYELSDLMRQATDAGVWPR"
BASE COUNT          478 a          696 c          721 g          463 t
ORIGIN      
        1 ttctggccta aagcggacat ccactcttgc gctgcacatg tccgctttca ggggtaaagc
       61 ggacatcacc gcctgacacg ggtctctgat cggcggaggc tactgtgacg ccacatcgtt
      121 gcgaccggag ttgcagttac agctcgggct cgatggcctt cagcaaccgg aagcgcagct
      181 ccccatcgtt gccggtcaac gagatcgcga agcgcacccg cccatttttg gacgtggtgg
      241 cgtagcctgc cagcgtgcgg acgccatcga gcgtgccggt cttgtacgag gcgctcttat
      301 ccttcttgag caggccggcg tggggcgcga agagactgag caccttggcg aggccgcgcg
      361 ccgtgaagcg gttgccgcgg ctgatgcccg agccttcctc gaggtaaata gccccggcga
      421 ggtcatgcgc ggcgagcatc tcgtttgcga ccttgagcga tttcttgaga ttgacgggac
      481 cgccgagacg gtgcccgccg atctccagga aaagctgatt ggcaatgtag ttgttcgagc
      541 cgacgagcag ctcgtggagg atctccggga ggctgcgcga ctggcgatgc acgtagactg
      601 gcgtcaggcc cgcgggcacg gtcccggttg cgatcttgcc tttgacgctg ccgccggcgc
      661 gctcgatgaa cgcggcgatc agttcgccgg catatcgcag gctcactgcg gggtcctgag
      721 ccagactgat gcggccggat cctttcggcc cccgtgcgcg gaactggctg atcgccagcg
      781 gcgtgatcgg ggtctgcgtc tcgccggaga tcacgttgtt gcccttgcga acggcggcga
      841 tcgtgttgaa gttcaccgcc agcgcggagt tcagcgcgtc gtaagacctg cgggtgtcct
      901 cgatgcccgg gatgcggaga tcggaaggat agtaacttgc gtcgagcacc atgccggtga
      961 tcggctgctt gccggccgcg gcgaccagtc cctgcgccag cccggcaagc tcttcggaga
     1021 ccaggaacgg atcgccgccc ccgcgcacat agaggacccg gcggtcgtcc aggtagaatt
     1081 ttgtctcgaa gcggtaatcc tcgcccaaca cctccagcac cagccaggcg gtcacgatct
     1141 ttgtgacgga cgccggaacg aagggcttgt ccgcgttctg agccaccagc tccttgccgt
     1201 cggcgtcgac cacgaacacc gtcgccgacg gggccagcgc cgcgaccttc tccttggcgc
     1261 cggccagcgc cggcgcgggc agcaggagca gagcaaaaag aagcaaacaa gaccgcatgg
     1321 cgagtgtgcg catggataca agcacgtcac acaggtggga cagggctacc gccaccctga
     1381 tccgagtcca gtttagtgac gataacaaaa tcagtcgact tcgcataaaa cctcgctccg
     1441 agcgcaaaat acgtcagcaa cgttcaccga ccggaaggct ttctccaatg ttatgatggc
     1501 catggcgaac aagcggacag tttgcatgag agaaaacctg gacatcgaca ccaagacgct
     1561 cgcgctttat tacgcctggg atcactttgc ctatcacgcg cagcaacgtc agaccgtctt
     1621 caattttttc ctgctggtgg tcggaggctg tgtcgccgct tattcggcaa tgatcggcga
     1681 gagcggaacg gaatatagtt atttccggag ctttctgggt gctgtcctgg cgctggcgag
     1741 cctgctgttc tggcgtctcg accagagaaa tgcgcggctt gtgaatgtgt ccgaggaggc
     1801 cttgaaaaaa cttgaagcgc ggatggcaga tgaaatcaaa gagccaagca tccggctgat
     1861 cgagaacgcc gacaaaaaga gcgcccgatt tctgttttca aagattgaat ccttcagcca
     1921 gatttatggg gtcgtctttg ctgtcagcgg actggttggg gtagcgatgg cgaccctttc
     1981 ggtgagggga tgagaggtaa tcccgtagcg aaatggcagc aaaagtaatt cgcgcacttt
     2041 gtgatcctcg tcactgaaca cccatcgccg gccaactact ctcaacctga ctttcacagg
     2101 gtcaggatgg gagatgacca tgaacacgat tacaaatgag accgccgagg tttcttccgg
     2161 cacctatttc gcacacttgt tcaagctgga tgccgatcag taccagctgt tacaaaagcc
     2221 gcgcttccat cgggactgcg attatctcaa tcgcccctac gaattgagcg acctgatgcg
     2281 ccaggccacc gatgccggtg tttggccgcg ctaataagat ctagacgcga gcccacgtga
     2341 acgccgtgac ggctccct
//
LOCUS       DASULZ010000049         4232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_918583, whole genome shotgun sequence.
ACCESSION   DASULZ010000049 DASULZ010000000
VERSION     DASULZ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918583"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VFY96_00955"
     CDS             complement(<1..540)
                     /locus_tag="VFY96_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5605050.1"
                     /translation="MPKLSAKKKLKTASIADIAHVGPGTPCGEWFRRYWVVVGIARDL
                     YDIPQAVKVLGEDLVLFRDEFGKLGLLGLHCPHRGTSLEYGDIESGGLRCPYHGWLFD
                     VTGQCLEMPAEPKDSKFPQKVKHLSYPVQELGGLIFAYMGADKDHPPLLPKYAPLLDR
                     GGQRQIEPVRQADYSWFNFF"
     gene            complement(770..1675)
                     /locus_tag="VFY96_00960"
     CDS             complement(770..1675)
                     /locus_tag="VFY96_00960"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5605051.1"
                     /translation="MVSYVRSLNMRIIVTGGSGRVGQAVVRELFTHGHRVAVLDVVKP
                     RECVCPTYVVDLSKTKALESHFESVEAVVHLARERFPYTETGFNTETQLWDCPDPLGD
                     GERFNLNVSMVNNVLAASRAAGVKKIVCGSSLAVYGFYYPRTAVQPDYLPIDEDHPLR
                     PHDPYGLTKLVGEKLCDAAAANSDIQIASLRFSGIYTEAHRGLILERKKKPLIRGTGA
                     LWSYVDARDAARACRLALGGEFTGHQAFNICAAETFMDAPTAELLQKYLPAVKLARPI
                     TGRQSGYDVSKAKRVLSFEAMLKLP"
     gene            complement(1651..2640)
                     /locus_tag="VFY96_00965"
     CDS             complement(1651..2640)
                     /locus_tag="VFY96_00965"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605052.1"
                     /translation="MRLRRRLTIIVPLLGAAAIFLSGLATAAPLRIAYSAISGAMATL
                     WVAQEAGYFKREALDTELLYIGGGSLLIQSMLSGEVPFAYGPSVPVINAALRGADLVL
                     VANTGNALVFSIMSRPEIKQPANLKGKKIGVTRLGGSTDWALAAALKQWGIQRRELLV
                     IQTGGMPEGLAGLASGSLDAVVLSPPSNFRALKAGMHELVDVGQLGIVFPNTPLSTTR
                     SFTKSQQDTVLRFLRGFTEGLNRLRTDKLFSMKVLSKYTKVVDSETLAEMYQTYGVRY
                     SGDPIPYVRAEGVEEILKRTPGKEAQDAKPGEFIDNSLLKDLEQSGWFRMFGR"
     gene            complement(2642..3871)
                     /locus_tag="VFY96_00970"
     CDS             complement(2642..3871)
                     /locus_tag="VFY96_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX5605053.1"
                     /translation="MAERIFDRRTKGVSIADIPHVGPLTAAGEWFRRYWIVVGATRDV
                     YDIPHAVKILGEELVLFRDPAGNFGLLNAHCPHRGASLEYGDIEDGGIRCPYHGWLFD
                     VRGRCLAMPAEPKESNFPGQVKHLSYPVREQGGLLFAYLGPQPENPPPLPKYQALADN
                     TGQRSLEATRIYDYNWFNFIENGADPVHFSILHRADPHDRTWRSWFFNYHEIPDFDAV
                     ETKYGMKVISRKPGPSPDTEYIDEKSFALPSILQIGDTEFTHFKQPREAVIAGSHNAH
                     FMFVTPNDDQSFTLFTVNHYTGADPEFFEKLAPSRRVEVEAEKKSYDQRRYSPFRGHV
                     RTEDIVCQSTQPLLSARKEQLASSDKGVILLRKIILNAIKAAQKRQTPKGLPLPEDAD
                     EIVRIDSFTGVRAKGGS"
BASE COUNT          967 a         1214 c         1085 g          966 t
ORIGIN      
        1 gaaaaaattg aaccagctat aatccgcttg ccgcaccggt tcgatctgac gctgtccgcc
       61 gcgatcgagc agtggcgcgt acttgggtaa aagcggcgga tggtctttgt cagctcccat
      121 gtacgcgaaa atcaacccgc caagttcttg caccggatac gaaagatgtt ttaccttttg
      181 cggaaacttg ctgtcttttg gctccgcagg catctccaga cattggccag tcacgtcgaa
      241 aagccagcca tgataagggc agcgcaggcc gccactttcg atgtcgccgt attccaacga
      301 tgtcccgcga tgcggacagt gaagcccgag caagccaagc ttgccaaact catcgcgaaa
      361 caacaccaga tcctcgccga gcactttgac cgcttggggg atatcgtaga ggtcgcgcgc
      421 gatgccgacg accacccagt atcgacgaaa ccattcaccg cagggcgtcc ccggcccgac
      481 gtgcgcgatg tccgcgatcg aggcagtctt aagttttttc ttcgcgctca gcttcggcac
      541 taacccttct tttttgccac tgttcggaaa gatctctcac gtttgtacga gatgacagat
      601 gcgtactgga tctcattccg agtggatgcg aggaatcttt cccaaatttc aattgaaaca
      661 aactcatccg cttgaacaat ttgaacgttt cgaacggttt aacgcttttg tctatttgaa
      721 atcattcgtt ccgctctttc aaaacgatta gttcctgcgt gaatcactgc tatggcagct
      781 tcagcatcgc ctcaaagctc agcacgcgtt tcgccttcga cacgtcatag ccgctctgtc
      841 tgcctgtgat tggccgcgcc aatttgactg caggtaaata cttttgcagc agctctgcag
      901 tgggtgcatc catgaaggtt tccgccgcgc aaatattgaa cgcctgatgc ccggtgaatt
      961 cgcctccgag ggctaaccga catgcgcgcg ccgcatcgcg cgcatccaca taggaccaca
     1021 gtgcaccggt gccgcggatg agcggcttct tttttcgttc caaaatgagc ccgcgatgcg
     1081 cctcggtgta aattccggaa aagcgcaaac tcgcaatctg aatatcgcta ttggccgctg
     1141 cggcatcaca gagtttttcc ccaacgagct tggtcaagcc gtacgggtcg tgcggtctca
     1201 gcggatgatc ttcgtcgatc ggtaagtagt cgggctgaac tgccgtgcgc ggataataaa
     1261 aaccgtacac agcaaggctc gagccgcaga cgattttctt cacgcctgca gcgcgcgacg
     1321 ccgccaaaac attattgacc atcgagacgt tcaaattgaa ccgctcgcca tcgccaagtg
     1381 gatcaggaca atcccagagc tgcgtttccg tgttgaagcc ggtttcggtg tacggaaagc
     1441 gttcccgagc caggtgaacc acggcctcaa ctgattcgaa atgtgattcg agcgccttcg
     1501 ttttactcag atcgaccacg taagtgggac aaacacattc ccgaggcttg acgacatcga
     1561 gcacggcaac tcggtgcccg tgagtgaaaa gctcccgcac aaccgcttga cctacgcgcc
     1621 cggatcctcc tgtgacgata atgcgcatat ttaacgaccg aacatacgaa accacccgct
     1681 ctgttccagg tctttgagca ggctgttatc aataaattca cccggcttgg cgtcctgcgc
     1741 ctctttgccg ggagttcttt tgagaatttc ctcgacgcct tccgcgcgga cgtacggaat
     1801 aggatcgccg ctgtaacgca ctccataagt ttgatacatc tccgccaaag tttcggaatc
     1861 gacgactttg gtatacttcg agagcacctt catgctgaac agtttatcgg ttcgcagccg
     1921 gttcaatcct tcagtgaagc cgcgcagaaa tcgcagcact gtgtcttgtt gtgacttggt
     1981 aaaactccgc gtcgtcgaca acggcgtatt cggaaagacg attcccaact gaccgacgtc
     2041 cacaagctcg tgcatacccg ctttaagggc acgaaaatta ctcggcggcg acagtacaac
     2101 cgcatcgagg ctgccggacg caagaccagc caacccctcg ggcatgccgc cggtctgaat
     2161 caccagcagt tcgcggcgct gaattcccca ttgcttgagc gccgcagcca aagcccaatc
     2221 cgtcgagccg ccgagacggg tcacgccgat tttcttgccc ttgaggtttg ccggttgttt
     2281 gatctccggc cgcgacataa tcgagaaaac cagtgcattg ccggtgttgg cgacgagaac
     2341 cagatccgca ccgcggagcg ccgcattgat gaccggcacg gatggtccgt aggcaaacgg
     2401 cacctcaccg ctcaacatcg actgaattag taaagagccg ccaccgatgt aaagcagttc
     2461 cgtatccaga gcttcacgct tgaaatatcc cgcttcctgc gcgacccaca gagtggccat
     2521 tgcgccgctg atcgcgctat aagcgatacg cagcggtgca gcagtagcta ggccggagag
     2581 gaagatggca gcggcgccga gcagcggcac gatgatcgta agccggcgac gaagtcgcat
     2641 ctcacgaccc tcctttcgca cgcacgccag tgaaagaatc gatcctcaca atttcatccg
     2701 cgtcctccgg caggggtaat cccttaggtg tttgtctctt ttgcgcggcc ttaatcgcat
     2761 tgagaatgat tttgcgcagc agaatcactc ccttgtccga acttgcgagc tgttctttgc
     2821 gcgcgctgag caacggctgg gtggattggc aaactatatc ttcggtgcgc acgtgccctc
     2881 taaacggcga atacctgcgt tgatcataag atttcttttc cgcttcgact tctactcttc
     2941 ggctcggcgc caatttctcg aagaattccg gatctgctcc ggtgtagtga ttgacggtaa
     3001 acagcgtaaa cgactgatca tcgttcggcg tcacgaacat gaaatgcgcg ttatgcgacc
     3061 cggcgatcac tgcctctcgt ggttgcttga agtgagtgaa ctctgtatcg ccaatctgca
     3121 gaatgcttgg taaggcaaaa ctcttttcat cgatgtattc ggtgtcgggg ctcgggcccg
     3181 gtttgcgcga gatgactttc atgccgtact tggtttcaac cgcatcgaag tcagggattt
     3241 catgataatt gaagaaccag ctccgccacg tgcgatcgtg aggatcggcg cgatgcaaaa
     3301 tcgagaaatg caccggatcg gcgccgttct cgataaagtt gaaccagttg taatcgtaga
     3361 tccgcgtcgc ttccaatgag cgttgtccgg tgttatcggc cagtgcctgg tattttggca
     3421 gtggcggggg gttttccggc tgcgggccga gataagcgaa tagcaaaccg ccctgctccc
     3481 gaacgggata cgataagtgt tttacctggc ccggaaagtt gctctccttc ggttccgcag
     3541 gcatcgccaa gcatctgcca cgcacgtcga acagccaacc gtgataggga cagcggatac
     3601 cgccgtcttc gatgtcgcca tattccagcg aagcgccacg atgtggacaa tgggcattca
     3661 acaaaccgaa atttccagcc ggatcacgaa acagcaccaa ctcctctccc aaaatcttga
     3721 ccgcatgcgg aatgtcgtaa acatcgcgcg tcgcaccgac cacgatccag tagcggcgaa
     3781 accactcgcc cgcagccgtc agagggccga catgaggaat gtcagcgatg ctgacgcctt
     3841 ttgttcttct atcaaatatt cgttctgcca ttcgatgttg cagagactgt gcgtgagccg
     3901 atcaagtgtt cggtcctgaa ctgagattaa acgaaaccgt cacgttactc cacaacggcg
     3961 cctttttgca cgctttttgc ctatagatat cccccatggg tcaagcgagc tcaagcgtcg
     4021 aagcgttaat cgaaatatta gacttgtgtg aacaggcact tacggcaatt cgtagctgtg
     4081 gagaacggct aatcccggaa ccaatgacgg acaaatatcg ccaatcctgg cgtaacacgc
     4141 gcgtaaagct cgatcgaaac tttgttctga ttcgcggtcg attggacaac caaactagtc
     4201 cctccaatcg ccgttagact caagtattcc tt
//
LOCUS       DASULZ010000050         5166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1324682, whole genome shotgun sequence.
ACCESSION   DASULZ010000050 DASULZ010000000
VERSION     DASULZ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324682"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..591
                     /locus_tag="VFY96_00975"
     CDS             151..591
                     /locus_tag="VFY96_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605054.1"
                     /translation="MGKSRFSRKDQTPFDDANRETAQNGRRQRACGGEAESPNVALTL
                     KHFHRKENSLTSAEGKRLYLRVGDEVNHLRYDEWGMGVVMEVMTSSVPGGTCLARVRF
                     QDGQLRCFNNDLDNEACCYYFGLRRYWNPTHGVDAIRTKFFLRG"
     gene            595..918
                     /locus_tag="VFY96_00980"
     CDS             595..918
                     /locus_tag="VFY96_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605055.1"
                     /translation="MLRPIKLFAAASILSMLCLWCPPAHAEDAIETAGVAVGVTAGNM
                     WFLPSKAISMSVGALSGALSYILTGNADLTKQIWQDTSQGPYLITPSLARTAIGQRPE
                     LLEQK"
     gene            complement(977..1711)
                     /locus_tag="VFY96_00985"
     CDS             complement(977..1711)
                     /locus_tag="VFY96_00985"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5605056.1"
                     /translation="MAQTIDNIVDPNDASLTSEMVSFPSQAGNVNAYVSRPKNGSNLG
                     TVIVIHENRGLVGHIKDVARRFAKDGFAAIAVDCMSRIGGSDKWNGSDEATKEIQKVN
                     GSMVAEDLASAVAYMKKQNYVNGKFGVVGYCWGGGQSLNFATKCKDLNAAVVYYGRNP
                     DPLDSVSNIPCPVMGNYAADDPNIMPGVEPLKAAMEKAGKKFDVKVYPGAKHAFNNNT
                     NADRYNPEAAKDAWARTTSFFKANLS"
     gene            complement(1892..2227)
                     /locus_tag="VFY96_00990"
     CDS             complement(1892..2227)
                     /locus_tag="VFY96_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX5605057.1"
                     /translation="MPIYEYQCQKCGTFEATQRITDKPLAKCPTCKGKVKKLISNTSF
                     QLKGTGWYVTDYARKGQNGDAKPEAKPADGTKETKEAKESKGESKKTETSSSSSEKSS
                     SSASSSTPA"
     gene            complement(2307..2663)
                     /locus_tag="VFY96_00995"
     CDS             complement(2307..2663)
                     /locus_tag="VFY96_00995"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5605058.1"
                     /translation="MDVKNLDEMIATKKRNIRLFGTGNFRSWMLYFVPGDGTDMHYHT
                     NPETFLVLSGSGVVKGLKGEERPIRKNEVLFLEAKDYYQITNNGTEPLVLFGNRSEGF
                     GGPHIKEDGSDRNATA"
     gene            2811..3554
                     /locus_tag="VFY96_01000"
     CDS             2811..3554
                     /locus_tag="VFY96_01000"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX5605059.1"
                     /translation="MLKLNGSQAAEFVAELAQKCDRRWQETVDQTRFMNELRDGKLKK
                     QTIQLFYKNWGSFVPVINSVYTSAFYRHLWFFVKNVDLMEVYTQKVLDEFGHPSPPGH
                     IQILMSTGKALGLSKEEVLLSPMLPACRALPDFHRTLLNDGQIQEYWFSVLWERPFGN
                     SCLDFYKALTRHYGLTTADASYFSKHHEADTMDHLDRKSHGAVTQTVLIRLLQEGINE
                     RPGYSAEYCVVTPADLNALFMNACYEATH"
     gene            3777..>5166
                     /locus_tag="VFY96_01005"
     CDS             3777..>5166
                     /locus_tag="VFY96_01005"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003882280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX5605060.1"
                     /translation="MAFADLRAFIDGAARIGEIKVIEGADWNLEIGCLTELMAEQEGP
                     LLLFDTIVGYPKGFRIATNALATPRRFALALGLPTEISKLEILKTWRSKTRDLKPFAP
                     VDVSSGPITENVLEGDRIDLSIFPTPKWHEHDGGRYIGTGDMVVIKDPESAWVNIGTY
                     RSCIVGKDRLTLWIIEQKHGKQIARKYWQAGRACPVAIVLGCEPATWMAAPSAAKLGV
                     SEYEYAGALRGAPLEIIRAPYTGLPVPATSEIVIEGEMPPPEEESHPEGPFGEWPGYY
                     THSGDECVVRVKRILHREHPILLGNPPLLPITERYGIPLYAARIWDHLEQSGISDVQG
                     VWCHCHTLMVVFSLKQRHVGQALQALTAVAGMQTGASMYRYYIAVDDDIDPVDLKQVI
                     WAMCTRVDPAESVQIIKSWTSDLDPRLPPEKRERGDFTMSRMLIDACKPFPWRDRFAI
                     ANKFSAEKREEVW"
BASE COUNT         1126 a         1430 c         1393 g         1217 t
ORIGIN      
        1 acttcaagct ccgagcagcc aagcgaagtt ggcgcatcat gagagcttga ctcttgaacg
       61 ggatttcagg aattatgggc gtgcgctgat tgagatcggc aacgagttat gcaagcttga
      121 atagaaatac gatttgatca aggatgattt gtgggcaaga gccgtttctc acgaaaggat
      181 cagacgccgt ttgatgacgc taaccgcgaa accgcacaga acggtagaag gcagcgcgcc
      241 tgcggcggcg aggccgagag cccgaacgtg gcattgacgt taaagcactt ccaccgcaag
      301 gagaattccc tcacatccgc cgaaggcaag cggctctact tgagagtcgg cgacgaggtc
      361 aatcacttgc gctatgacga gtggggcatg ggcgtagtca tggaagtgat gacatcctcg
      421 gttcccggtg gcacttgcct tgctcgggtg cgctttcaag atgggcaact gcgttgcttc
      481 aataacgacc tcgacaatga ggcatgctgc tattacttcg gcttacgccg ctattggaat
      541 ccgacccacg gcgtagatgc tattcggacg aaattttttc tcagaggtta gctcatgctg
      601 agacccatta agctattcgc cgcggcatcg atcctgtcga tgctttgtct gtggtgtccg
      661 cccgcccatg ccgaggatgc aatcgaaacg gccggcgtcg cggtcggcgt caccgctgga
      721 aacatgtggt tcttaccgag taaagccatt tcgatgagtg ttggcgcact gagcggcgcg
      781 ctctcctaca ttttgaccgg caacgccgat ttgaccaagc agatttggca agatacctcg
      841 caaggtcctt atctgatcac tccgtcgctg gcgcgaacag ccattggtca gcgtccggag
      901 ctgctcgagc aaaaataaaa aacggcatgc gaccggagaa aagccgcatg ccgtcgtgat
      961 cggtccgtct gtcggtctaa cttaaatttg ccttgaagaa gcttgtcgtc cgcgcccagg
     1021 catccttggc ggcctcggga ttgtatcgat ctgcgttcgt gttgttgtta aacgcgtgct
     1081 tcgcgccggg atagacttta acatcgaact tcttgccggc cttctccatc gctgccttca
     1141 gtggctccac ccccggcata atgttcgggt catccgccgc gtaatttccc atgactgggc
     1201 agggtatgtt gcttacggaa tcgagcgggt caggatttcg accgtagtaa accactgcgg
     1261 cgttcaggtc cttacatttg gtggcaaaat ttagggactg gccgccgccc cagcagtagc
     1321 ccaccacgcc aaactttccg ttcacgtaat tctgcttttt catgtatgcc acggcagacg
     1381 ccaaatcctc ggccaccatg ctgccgttca ccttctgaat ttccttggtg gcttcatcgc
     1441 tgccattcca tttatcgctg ccaccgatcc gactcatgca atccacagcg atcgcagcga
     1501 aaccgtcttt ggcaaaccgg cgcgccacat ccttgatgtg accgaccaat cctcgatttt
     1561 cgtgaatcac gatcacagta ccgagattgc tgccattctt tggccgggag acgtacgcgt
     1621 tcacgtttcc tgcttggctt gggaacgaca ccatttccga tgtcaggctg gcgtcattcg
     1681 gatcgacgat attatctata gtttgagcca tcgataaaaa cctccttcag caaattttct
     1741 tgtcaatttc gtcccagcta tagcgaaaaa caaatttcaa atctagacct attgacgcga
     1801 attccgggct gtcggagagt aaaaataact gcggcgcccc gaggcgccgc agtgcacaaa
     1861 accggtaact gtttgtatgg ctgattggga gttatgcggg cgtagaactc gaggcactgg
     1921 acgaagattt ttcgctgctc gaggatgaag tctcggtctt tttactttcg cccttggatt
     1981 ctttggcttc ctttgtttcc tttgttccat ccgcgggctt ggcttcgggt ttggcgtcgc
     2041 cattctgtcc cttgcgggcg taatccgtga cgtaccagcc ggttcccttc aattggaacg
     2101 aggtgttcga aattagcttt tttacctttc ctttacacgt cggacacttt gccagcggtt
     2161 tgtcggtaat ccgctgggtg gcctcaaagg taccgcattt ttggcattgg tattcgtaga
     2221 taggcatgca aaggtcctca atcgaagcta taatcgcaaa aagtaaccac tacccggcgc
     2281 attgtcaatc agccgaagcc gcagaattat gccgtggcgt tacgatccga gccatcttct
     2341 ttgatatgcg gccccccgaa cccctccgaa cggttgccga agagcaccag cggctctgtg
     2401 ccgttgttgg taatctggta atagtccttg gcctccagaa acagcacctc atttttccgg
     2461 atcggccgct cctctccctt caagcccttg accacaccac tcccggacaa aaccaaaaac
     2521 gtctcgggat tggtatggta gtgcatgtcg gtgccgtcgc caggaacgaa ataaagcatc
     2581 catgaccgaa aattgccggt gccaaacagg cggatattac gcttcttggt cgcgatcatc
     2641 tcatcgaggt tttttacatc catcgtttcc tcctcaagct agatggactc gattgtatcg
     2701 tagctctttt atcttcggca agccttgccg cagcgagctt gacagctgtc tgggcctgca
     2761 ataggggtga cgatgtttgc cgcaatagtg catcttcgag gaggctgtta atgctgaaat
     2821 tgaacgggtc gcaagccgcg gagttcgtcg ccgagctggc gcaaaaatgc gatcgccgct
     2881 ggcaggaaac cgtggatcag acacgcttca tgaatgagtt gcgcgatggc aagctcaaga
     2941 agcaaaccat ccagctcttc tacaaaaact ggggctcctt cgtgccggtg atcaactccg
     3001 tctacacttc ggcattttat cgccaccttt ggtttttcgt gaagaacgtt gacctgatgg
     3061 aagtctatac acaaaaggtt ctcgacgaat tcggtcatcc ctcaccgccg gggcatattc
     3121 agattctcat gagcacagga aaggcactgg gcctcagcaa agaagaagtc ttgctttcac
     3181 cgatgctgcc ggcctgccgc gcattgccgg attttcaccg aacactcctc aacgacggtc
     3241 agatccagga gtattggttc tcggtgctat gggaacgccc gttcggcaat tcctgcctgg
     3301 atttttataa agcgttgacc aggcactacg gcctcacgac cgcggacgcg agttactttt
     3361 ccaaacacca cgaagcggac accatggacc atctcgatcg caaatcacat ggcgccgtga
     3421 cgcaaacggt cctgatccgt ttgttgcagg agggaatcaa cgaacgtccg ggctattcgg
     3481 cggaatattg cgtcgtcacc cccgccgatt tgaatgcact cttcatgaac gcctgctacg
     3541 aagcgaccca ctaaaggagc tgcgccgttt ttgacatttc aaatttcata tctcagatcc
     3601 gttccgagcg tcctagctga gattgtcatt gagattcgag gctaaaggcg gtgtcgcagt
     3661 tgtcatttcg agccgaatgc gatgggtctc gtctcccatc gagattcttc ggctgcgcct
     3721 gagaatgata tctaacgttc agagttgaca ttagagttct ccacgaagtg agcactatgg
     3781 cgttcgctga tctaagagcg tttatcgacg gtgcggcgcg catcggcgag atcaaagtta
     3841 tcgaaggcgc ggactggaat cttgagatcg gctgcttgac cgagctgatg gcagaacagg
     3901 aaggtccgct gctgctgttc gacacgattg tcggttaccc caagggtttt cgcatcgcca
     3961 ccaacgcgct cgccacaccg cgccgcttcg ctctcgcgct tggtcttcct acggagatat
     4021 cgaagctgga aatcctcaaa acctggcgca gcaagacccg cgatctaaaa ccatttgcgc
     4081 cagtggacgt ttcatcaggg ccgatcacgg agaacgttct cgaaggcgac cgaatcgatt
     4141 taagtatttt cccgacgcca aaatggcacg agcacgatgg cggccgttac atcggcacgg
     4201 gtgacatggt ggtcatcaaa gatccggaga gtgcctgggt caatatcggc acctatcgat
     4261 cgtgtattgt cggcaaagat cggctgaccc tgtggatcat cgaacagaaa cacggtaaac
     4321 aaatcgcccg caagtattgg caagccggcc gcgcttgtcc cgtcgcgatt gtgctcggct
     4381 gcgagcccgc cacgtggatg gcagcgccgt cggcagcaaa actgggtgtc agcgaatacg
     4441 aatatgccgg cgcgttacgt ggcgcgccgc ttgaaatcat tcgtgcgccg tataccggat
     4501 tgccggtccc cgcgacttcg gaaatagtta tcgaaggcga gatgccgccg ccagaagaag
     4561 agtcgcatcc ggagggcccc ttcggagagt ggccgggata ctacacccac agcggcgacg
     4621 aatgcgttgt tcgcgtcaag cgcattcttc atcgcgagca tccgattttg ctcggcaatc
     4681 cgccgctgct cccgatcacg gagcgctacg gtatcccgct ctatgcggcg cggatctggg
     4741 atcatttaga gcagtcgggc atttcggacg tccagggcgt ctggtgccac tgccatacgt
     4801 tgatggtggt gttttccttg aagcagcgtc acgtcgggca ggcactgcaa gcgctcaccg
     4861 cggtggccgg catgcagacc ggcgccagca tgtatcgcta ctatatcgcc gttgacgatg
     4921 acatcgatcc ggtggattta aagcaagtca tctgggcgat gtgcacgcgc gtcgatccgg
     4981 cggagtcggt gcagatcata aagtcctgga cttcggatct cgatccgcgg ctgccgcccg
     5041 agaaacgcga aaggggcgat ttcaccatga gccggatgct catcgacgcc tgcaaaccgt
     5101 ttccctggcg cgaccgcttc gccatcgcaa ataaattcag cgccgagaag cgcgaagaag
     5161 tgtggg
//
LOCUS       DASULZ010000051         2855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_918745, whole genome shotgun sequence.
ACCESSION   DASULZ010000051 DASULZ010000000
VERSION     DASULZ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918745"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VFY96_01010"
     CDS             complement(<1..197)
                     /locus_tag="VFY96_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605061.1"
                     /translation="MRSLRLLCSGLIAGLVLFQWLSIARAAPLDELVGAARKEGTLNF
                     YGPSTLKPEGAQVIAQAFNKR"
     gene            complement(601..1653)
                     /locus_tag="VFY96_01015"
     CDS             complement(601..1653)
                     /locus_tag="VFY96_01015"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605062.1"
                     /translation="MNQKKSSLSSIGSATFISTIALAVLICGASAGKLFAAAADSLDQ
                     LYEKAKKEGKVTLYAPLSPSAMSVIPPAFMKRFPGITVDHIDATSDKLIARVLAEQKG
                     GRVIADVISGALTYLPQILDLKIVERVVIPEAAAYPAQLKGDLWVATDTQFYIAGWNT
                     NLVKKGEEPKSYEELANPKWKDSIMGEPRDYQMLVGFAKRKYNSDEKAIDLFKKIAAN
                     QVEFHRGHSQLTEFLLAGQRAICFTCYSHHFPMRVKKGAPIQPLMTEGVGEVGGSAMV
                     MKEAPHPNAALLWVRWAVSEEGQKVYAQAGETPAHPKVEPLEKIRPTTPYMLTIDDVR
                     EFPKYEKTWKEIFQIR"
     gene            complement(1884..>2855)
                     /locus_tag="VFY96_01020"
     CDS             complement(1884..>2855)
                     /locus_tag="VFY96_01020"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605063.1"
                     /translation="FVLVWIGCASVTVAGAQLDSVTIGFSSFSGFYGPLWLAVEDGLG
                     KKYGVDLRAVYAGRVRPQQLLASGETPLVIASGSGAMTSHVLGVKDQVIVALLSSKMG
                     GGIFSKSEIKRPEDLKGKLIATGRPGALNDVLVRYVLTRKWGLSPDRDVKLMPIGEPP
                     LMLQALERGVVDATSLSVPASFLAKSKGFFELVNYDALGLSYPQHAVTTLRPTIAKTP
                     QMIEGVLKALIESVAIFKTNKQKSFAVWRKYMRGASEEFLEETYQHTIAGLESVPTPS
                     VQVIASALDMLSAHYPQAKQTDPNLIVDPSFVRRIDQSGFIAALYKK"
BASE COUNT          635 a          790 c          736 g          694 t
ORIGIN      
        1 tatcttttgt tgaaggcttg agcgataacc tgagcgccct caggcttcaa ggtggacggg
       61 ccatagaagt tcagcgtgcc ttcttttctg gcggcgccga ccaattcatc gagcggcgcg
      121 gcgcgagcaa ttgagagcca ctggaacaaa accaagccgg caatcagtcc cgagcaaagg
      181 agacgcagcg atcgcatctt tcacctcccg ttacgtgccg tggccctttc caggagccgt
      241 ctggtaaatg cgcccgagct ctttcctgga cggcgtctat cgctggtcct gagaattgtc
      301 aagtcgatat ttcacgctgt cctcttgctc gcgcctattt gacggaatgc aagcgatggc
      361 tatgccgagc ggcagcccgg aaagcgaagg aatcagttct tagatcggcg agctaaattc
      421 actgtgcagc ttgggatcgc ggtcccgtcg attagcgaga tggcgatcga tagtcgaaaa
      481 gcaagttatc caatgccacc cctaaaatgt tcatcgtatg tacctttgag atctagtgca
      541 ccggagcaag tcatcgacag gttgccgcgg cccgtcgcaa taaagaccgc tcgtcgaatt
      601 tcaacggatc tggaagattt ccttccacgt tttctcgtac ttcggaaact ctcttacgtc
      661 gtcgatcgtc agcatataag gcgtcgtcgg gcgaattttt tccagaggtt ccactttggg
      721 atgagccgga gtttcacccg cttgagcata aaccttctgt ccttcttcac tgaccgccca
      781 gcgaacccag agtagtgcgg cgttgggatg cggagcttcc ttcatcacca tcgcagagcc
      841 gccgacttcg cccacacctt ccgtcattag cggttgaatc ggggcgccct ttttcactcg
      901 catcggaaaa tggtgtgagt agcaagtaaa acaaatggcg cgctgaccgg ccaagaggaa
      961 ctcggttaac tgcgaatgcc ctcgatgaaa ctcaacttga ttggcggcga ttttcttgaa
     1021 cagatcgatc gccttttcat cgctattgta tttccgtttg gcgaatccga ccagcatttg
     1081 gtaatcgcgc ggctcgccca tgatgctatc tttccatttg ggattcgcga gctcctcgta
     1141 gcttttcggc tcttcgcctt tcttcaccag attggtgttc catccggcaa tatagaattg
     1201 cgtatccgtt gccacccaaa ggtcgccttt caactgggcc ggatacgcgg ccgcctcggg
     1261 tatgacgacg cgctccacga tctttaggtc caggatctgc ggcaaatagg tgagcgctcc
     1321 gcttatgacg tcggcaatga cgcgtccgcc tttctgctcc gcgagcactc gcgcgatcag
     1381 cttgtcgctc gtggcatcga tatgatcgac cgttataccg ggaaagcgct tcatgaacgc
     1441 cggcggaatc accgacatcg cgctagggga tagcggtgca taaagcgtaa cctttccttc
     1501 cttttttgct ttctcataaa gttgatcgag ggagtccgct gcagccgcga aaagcttacc
     1561 ggcagaagcg ccgcagataa ggacagctaa ggcgatggtc gagataaagg ttgcagatcc
     1621 gatcgaactc aatgaactct tcttttgatt catttcactc ctccgtgatc ttcattccat
     1681 gatgctgaat caatatccgg gaaaattttc cctgccttgc gactcttcgt aaacccttct
     1741 ctgcaactcc tccatgtagg aaaataccga agatgtcaaa cttaggccag gcattcatgt
     1801 ttcgctcatc ccactaggta gttccaaatt aaaccactaa gttttgcgag agggattcgc
     1861 ggctgcgcgc agatttgggt gagttatttt ttatataagg cggcgatgaa tccgctctgg
     1921 tcgatccgtc ggacgaacga tggatcgacg atgagattcg gatcggtttg ttttgcttga
     1981 gggtaatggg cggaaagcat gtcgagcgca ctggcaatca cctgcacgga aggtgttggc
     2041 accgactcca gtcccgcgat agtgtgctgg taggtttcct cgagaaactc ctcgctcgcg
     2101 ccccgcatgt atttacgcca aacagcgaaa ctcttttgtt tattggtttt aaaaatcgcc
     2161 acggactcga tgagagcctt caaaactccc tcgatcatct gtggtgtctt cgcaatcgtc
     2221 ggtctcaggg tggttacggc atgctggggg tagctgagcc ccagagcatc gtaattgacc
     2281 agctcaaaaa atcccttgct tttagccaga aagctcgccg gaaccgacaa cgatgttgca
     2341 tcgacaacgc cccgttccaa agcttgtagc atgagcggcg gctctccaat cggcatcagc
     2401 tttacatctc gatcgggaga gagtccccat ttgcgcgtca acacataacg aaccagaacg
     2461 tcgttaagag cgcctgggcg tccggttgca atcagcttgc ccttcaggtc ttcgggtctt
     2521 ttaatttcgc ttttggagaa tatgccgccg cccattttgc tggaaagcag cgccacgatc
     2581 acctggtcct tcacgccgag aacgtgtgaa gtcatggcgc cgcttcccga cgcaattacc
     2641 aagggagtct ccccgctggc gagcaattgc tggggacgga ctctgcctgc gtagacggct
     2701 ctcaggtcca ctccgtactt tttacccaaa ccatcttcga cggcgagcca caacggaccg
     2761 taaaatcctg aaaaggaaga aaacccgata gtaacgctgt cgagctgtgc gccggcgacg
     2821 gtgaccgacg cgcaaccaat ccagacaaga acgaa
//
LOCUS       DASULZ010000052         3289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_513245, whole genome shotgun sequence.
ACCESSION   DASULZ010000052 DASULZ010000000
VERSION     DASULZ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513245"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1691)
                     /locus_tag="VFY96_01025"
     CDS             complement(120..1691)
                     /locus_tag="VFY96_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5605064.1"
                     /translation="MAVKRSCQELADLMNSDDLFAVFDIRERGEYNQCQIPGTTSLPR
                     SQIEFRGSRLVPNPDISVIIYDQAEERAPLAAETLTELGYRNVSILRGGITAWSGENR
                     PTVSGVNVPSKAFGEKVHHERSIPEISADELFRLQKEHEPLRILDVRTPEEYGGFCIP
                     GAVNVPGGDLILWLDELRESTTPVIVNCAGRTRSIIGTAALRRLGLTNVRALKNGTMG
                     WVLSGLDLEKNPPRRTTQASEQSRTRAVSQALALAAEENIAWISPDEIAAAVANQTDT
                     VTYAIDVRSEAEYEFGHIAGSINVPGGQAVQRADDFVAVRNGQIIFISNDSVRAIMAA
                     YWYRQMGYPHVFVLRGGLRAWSGSGGQLETGMPQDEPLGFETAKRNAHLLGPEELARR
                     PRKSTMLILDVGTSVDFEAAHVPGANWISRGWLEIVIPERFRDRRQAIVLTCSNGAQS
                     IFAARALQRVGYTDVAALSGGVRAWSAAGFPTEAGLDRRLMEPNDVVLSPSVRGSRED
                     MQRYLDWEVRLTKPA"
     gene            1759..2736
                     /locus_tag="VFY96_01030"
     CDS             1759..2736
                     /locus_tag="VFY96_01030"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX5605065.1"
                     /translation="MSAIHERFSGLFVPHVTPFHDDGSVDLESLTRLTQYFAGIRSVA
                     GLVSCARIGESPVLNLEEKRRVYEISGKAMHDAGKVHIAAIAPQSTDEAVALTRDLEN
                     LPVDGVMIFPPLLFAWGKVDPNLKVRFFEDLARETKLPVVLFQIPVKSYYFDPETICR
                     IARLENVVAFKEASFDIDLFTQTVNALKHDRAAMRVLTGNDRFVAESYKLGANGALIG
                     VANLATEKWGALDIAGRAGRSDEAAAVQNELASVKELVFSEPIVEAVARIKIVLQHEG
                     LIKTDKVRRPQLGISEAEQKQLLDSYDALKRNDFRAGTAELEKKRQAVG"
     gene            complement(3039..>3289)
                     /locus_tag="VFY96_01035"
     CDS             complement(3039..>3289)
                     /locus_tag="VFY96_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605066.1"
                     /translation="VAFAALHHRNYRRYFFVIMLSQMGDNNMSNNGLHAFNGVTVGVV
                     GGLIGIHWSLALSAMILLAVTIALFALVIPGRGTGAFR"
BASE COUNT          727 a          975 c          881 g          706 t
ORIGIN      
        1 atcaggaaat tacgtagtgt cctgctgagc gcagtgaagc atcgccgctt ctgcgcttgg
       61 gaagaacgag attcttcggc ttcgccttgg tgatccgcgg ctgagagagg tatgccccat
      121 catgccggtt tggtcagcct aacctcccaa tccaagtacc gctgcatgtc ttccctgctg
      181 ccgcgaacgg atggtgacaa gacgacatcg ttgggttcca tgaggcgccg atcgagtcca
      241 gcctcagtgg gaaaacccgc tgccgaccag gcgcggacgc cgccgctcaa cgccgcaacg
      301 tccgtatagc cgactcgctg caatgcgcgc gcggcaaaga tcgactgcgc tccattgctg
      361 catgtgagaa cgatcgcctg tcgccgatcg cggaaccgtt cgggaatcac aatctcgagc
      421 cagcctcgcg atatccagtt cgctcctggc acgtgagccg cttcgaaatc gacgctcgtg
      481 ccgacgtcga ggatcagcat tgtcgatttc cttggtcttc gcgccaactc ttccggccca
      541 agcaaatgcg cgttccgttt ggcggtttca aaaccgagcg gctcatcttg cggcatgccg
      601 gtttcaagct gcccgccgct ccctgaccac gcgcgcaacc ctccgcgcaa aacaaaaaca
      661 tgcggatatc ccatttggcg gtaccaatag gcagccatga tcgcccgtac cgaatcatta
      721 ctgatgaaaa taatttgccc attccgcacc gcgacgaagt cgtcggcccg ctgcacggct
      781 tgtccgcccg gcacattgat tgagccggca atgtggccga attcatattc cgcttccgag
      841 cgcacatcaa tggcgtaggt cactgtatcg gtttggttcg ctactgccgc cgctatttca
      901 tcaggcgaga tccatgcgat gttctcttct gcagcaagcg caagcgcctg cgaaacggcg
      961 cgcgtgcgac tctgctctga tgcctgggtt gtccgtcgcg gcggattttt ttccagatcc
     1021 aagccagaca gcacccagcc catggtcccg tttttgagag cgcgtacgtt ggtcaatccc
     1081 aagcggcgca atgccgcggt cccgatgatg ctccgcgtcc gaccggcaca atttacgatc
     1141 accggggtgg tcgattccct gagttcatcc agccataaaa tgagatcgcc gccgggaacg
     1201 ttcacggcac cgggaataca gaatccgcca tactcttcgg gagtgcgcac gtcgagaatc
     1261 cgcaacggct cgtgctcttt ttgcagccga aacagttcat cggcggaaat ctccgggatg
     1321 cttcgctcgt ggtgaacctt ttcaccaaaa gctttgctcg gcacgttgac accgctcacg
     1381 gtcgggcggt tctcgccaga ccacgccgta atgcctccgc gcagaattga aacgttccga
     1441 taacccaatt ccgtcagagt ctccgccgcg agcggcgccc tctcctcggc ttggtcataa
     1501 atgatcacgg aaatatcggg gttcggcacc aaccggctgc cgcggaattc gatttgactg
     1561 cgcggcaacg atgtcgtgcc cggaatttgg cactggttgt attcaccccg ctcccggatg
     1621 tcgaaaacgg caaataaatc gtctgaattc atcaaatctg caagttcttg gcatgatctc
     1681 tttacagcca tggcttgaac tcataccgta atctggtcta cttatgctag cgactaattc
     1741 tagatcttcg gaggaagaat gtcagcgatt catgaacgat tcagcggctt gtttgttcca
     1801 catgtgacac cgttccatga cgacggctct gtcgacctgg aaagcctcac gcgtttgacc
     1861 caatatttcg ctgggattcg aagtgtcgcc ggcctggttt cttgcgcccg catcggtgag
     1921 agcccggtgc tgaacctgga agaaaaacgc cgcgtttacg aaatctccgg taaggcgatg
     1981 cacgacgccg gcaaagtgca cattgccgcg atcgcgccgc aatcgacgga tgaagccgtc
     2041 gccctgacgc gcgatctcga aaacctgcct gtcgacggcg tgatgatctt tccgccgctg
     2101 ctttttgcct ggggcaaggt cgacccgaat cttaaagtcc gtttcttcga agacctggcg
     2161 cgggaaacca aactgcccgt ggtgctcttc cagattccgg tgaaaagcta ttatttcgac
     2221 cccgagacga tctgtcgaat tgcccgtctg gaaaacgtcg tggcgtttaa agaagcgtcc
     2281 ttcgacatcg atttattcac tcaaaccgtg aacgcgctca agcacgatcg tgccgccatg
     2341 cgcgtgctta ctggtaacga ccgctttgtc gccgagagct acaagcttgg tgcgaacggc
     2401 gcgctgatcg gcgtcgccaa tctcgccacg gaaaaatggg gcgccctgga tatcgccgga
     2461 cgcgccggta gatccgacga agcggctgcg gtccaaaacg agctggcgtc cgtaaaggaa
     2521 ttggttttta gcgaacccat tgttgaagcg gtggctcgga tcaaaatcgt gctccagcat
     2581 gagggattga ttaaaaccga caaagtgcgc cgcccgcagc tcggcatctc agaagccgag
     2641 cagaaacagc ttctcgacag ctacgacgcg ttgaagcgaa atgatttccg cgccggaacc
     2701 gcggaactgg aaaaaaagcg ccaagccgtc ggctaacgcc cgatcaccgt aagggcgaaa
     2761 aaaaatcgcc tctacaagtc cctccaattt cgttttcgtg aacttcgcac cccgttttga
     2821 aaatcctagt gcgctaaaga gagctgggac ttccaaatcg tcaccccccg catgctttag
     2881 tcgggggtcc agtccgaatt ccgcctggat tcccgctaaa agcatgccgg aatgacggac
     2941 tgcggggaga atcgaggtta tgaagtgagg aaaatcctag ctttcgttct tttggggcaa
     3001 gcgtaagaca aatcacttcg ggcgagattt ctttttattc atctgaatgc gccggtaccg
     3061 cggcccggaa tgaccaatgc aaacagcgca atcgtcacag cgagcagaat catcgcgctc
     3121 agcgccaatg accagtgaat tccgatcaaa ccgccgacga cgccgacagt gacgccgttg
     3181 aatgcatgca agccgttgtt ggacatatta ttgtcgccca tctgcgacag catgatcaca
     3241 aagaagtagc gccgatagtt gcgatgatgc agcgccgcaa aagcgacct
//
LOCUS       DASULZ010000053         7042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_107261, whole genome shotgun sequence.
ACCESSION   DASULZ010000053 DASULZ010000000
VERSION     DASULZ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7042
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107261"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..643)
                     /locus_tag="VFY96_01040"
     CDS             complement(248..643)
                     /locus_tag="VFY96_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605067.1"
                     /translation="MERSPNLKIMAVLATLQGVLGLLRAYNWVQIGANLFGQGLLLVP
                     FVGVMAVARGVFISIVALLYVLFAIGVFLGKSWAWWPCITAVIINLFLVVGALVQGVP
                     LIEGIAWSVIPAVLLVFFFSQKGDAVAGV"
     gene            complement(785..982)
                     /locus_tag="VFY96_01045"
     CDS             complement(785..982)
                     /locus_tag="VFY96_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605068.1"
                     /translation="MVDGYGCHRGSDKVSYHCHQGQFAGRTFKSKDDFLRELRGGKAT
                     QLSPKNNPPQPERKPEKIAPN"
     gene            complement(1114..1977)
                     /locus_tag="VFY96_01050"
     CDS             complement(1114..1977)
                     /locus_tag="VFY96_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5605069.1"
                     /translation="MFKRTIIFGVLLMSLCLSRPSIGQELDGTLKKIKDSNTFTLGYL
                     TSAPPFSFPGPDKRPVGYSIDLCTQIASKIQDQLGTTLKLNWVPVTTENRLDMVASGK
                     VDIECGTTTASLSRQERVDFSLMTFVDGGGLLTKKDFTRRAVADLADKRIAIIPGTTT
                     ETAFAKFLKEEFVTVKLVPVKNHVDGLTALQNGSADAFASDRGILIGLAVTSKDPSVF
                     ALPPILFSYEPYGFMVRRNDAAFRLAVNRALAGLYRSGDVAPIYERWFGGFGKPSDAL
                     RAMYLINGVPE"
     gene            complement(2100..2591)
                     /locus_tag="VFY96_01055"
     CDS             complement(2100..2591)
                     /locus_tag="VFY96_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605070.1"
                     /translation="MQIDLFAKTIVVAMAFMLFGTAPLGVAQDKPADNMQILRDKIRA
                     DKKLVIATNMELTESEAKNFWPIYEQYQKDLQKINQRMANLLESYADDFRNKSLTDEK
                     AKKLIDEALAIDRAEATLKSTYAPKLSKALPIRKAVRYLQIENKIRAVVKYDIASGVP
                     LVK"
     gene            complement(2664..3263)
                     /locus_tag="VFY96_01060"
     CDS             complement(2664..3263)
                     /locus_tag="VFY96_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEX5605071.1"
                     /translation="MSFDYGRVRLRDAMAVIRLGCVICAMSLAINAAMLTMCLAASAK
                     TIDRDAKATLDKLYKNNQGAKALGDKAVGVLVFPSIVKGGFIIAGQFGDGALRKNGKS
                     VAYYRSLAASYGFQAGAQAFGYVLFFMDEDSLRYLDNSAGFELGTGPSLVVLDSGFGK
                     NLSTTTLQKGIYAFIFDQKGLMGGVGIQGTKITKINPNP"
     gene            complement(3424..3930)
                     /locus_tag="VFY96_01065"
     CDS             complement(3424..3930)
                     /locus_tag="VFY96_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605072.1"
                     /translation="MTRRTVPEFVLVGMLLGLMHLTHAWAQTKAPAGDKPASNLEIIH
                     EKLKADKKLTVAKYMDLTESEAAKFWPVYEEYQTDLQSSNEKLRKLLESYAADYRNKS
                     LTDEKAQKLLDEWIALEQEEGKRRSAFAPKVLAALPAKKAARYLQIENEFRILLRYDL
                     AATVPLVQ"
     gene            complement(4149..5225)
                     /gene="ydiK"
                     /locus_tag="VFY96_01070"
     CDS             complement(4149..5225)
                     /gene="ydiK"
                     /locus_tag="VFY96_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HEX5605073.1"
                     /translation="MADIQRDITRNLLAVIFILALIGSSIWILRPFLGAIVWATTVVV
                     ATWPFMISLQKWLGKKRVLAVIVMTLLLVCVLVVPLTFAIGAIASNLDDIAIWVKDLA
                     SFMLPPPPTWLASLPLVGAKAAELWERVAIVGIQDATVRVAPYAGGVIKWFAAQLGSV
                     GVLFLEFLLTIILAATMYASGERATERLLRFGRKLAGSGGENAIYLAGQTIRGVALGV
                     VVTAIVQSLFSGIGLIIAGVPFAAALTGAMLLLSIAQIGVIPVLGGVVAWLYWTGQSG
                     WGTFMVVWMLIAGTMDNFLRPILIRKGADLPLLLVFAGVVGGLIAFGLIGIFVGPVIL
                     AIADKLLMAWIEDDIHDGELRDGP"
     gene            complement(5309..6391)
                     /locus_tag="VFY96_01075"
     CDS             complement(5309..6391)
                     /locus_tag="VFY96_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605074.1"
                     /translation="MQRKTPSIMVWVLLTLGGCASVGPGTVARDRFDYISAISDSWKS
                     QMLLNLVKLRYGDAPVFLDVASVINQYGVEGSLGFSGSWAQNSQLPWPYTAVYNLLGS
                     GRYVERPTITYAPLSGEKFARNLMTPIPPAAILNLLQGGYPVDLVLRLCVHSINGINS
                     RFGSGARARQADTEFYELVERLRNIQQSGDLGLRVRKTGDQTTTMLVFSKKPNPAIDA
                     DRAAVRKLLGLDPKTEELNVVYGSVPINDKEIAMLTRSMLEILLDLSSYIEVPAASAT
                     ERRTFATPAPEIVNGTPVVPLIRISSSSQAPGDTFAAVPYGREWYWIDDKDFASKRLF
                     SFIMFLFTLTETEGKPNAPLITIPVG"
     gene            complement(6412..>7042)
                     /locus_tag="VFY96_01080"
     CDS             complement(6412..>7042)
                     /locus_tag="VFY96_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605075.1"
                     /translation="GAIYLPMAAATLAYTHLTPTEYDGGPYLALSVGAGVTWIIGLAA
                     VIWLIAYLIWSASRSRAVGPSAGNILNAVHPGEWWVVLAHAVAFGSGYVLGAANASYL
                     FVIAVHHEVQYLAFTCAMARRRTNSQSLAVQSAVQDVYSPGPCRADRGIRTEVSFIGS
                     FLFWPVIGFVGAVFGSWLNFPALAPFGAGGLFCHYWLDGRIWRRPSAHA"
BASE COUNT         1741 a         2032 c         1707 g         1562 t
ORIGIN      
        1 ttttcactga gaatcggcga atagtaaatc gccccgtcaa cccagacggt catcagcaga
       61 acacaaatcc cagccagaat ccggatgcca actctcactc aggctccgaa aaggactacc
      121 ggagtactcg cagtccgctc ataagctttt acttactctc cgaggttccc gagagctcga
      181 atctgactct accctgaccg tcggagcacg accattgctt tagtccaatg gtcaaattcg
      241 ttaagcgtta cacgccggcc acggcgtcgc ctttttgtga gaagaagaag acgagcaaaa
      301 ccgccggaat taccgaccag gcgattccct cgatcaaagg cacgccttgg accaaggcgc
      361 ccacaacaag aaataaattg atgatgactg ccgtgataca gggccaccat gcccagctct
      421 tccccagaaa cactccgatg gcgaacaata catagagtag agccacgatc gagataaaca
      481 cgccccgcgc tacagccatt acgccaacga acgggaccag cagaagccct tgcccaaaaa
      541 gatttgctcc gatttgcacc cagttgtatg cgcgcagcag acccaacaca ccttgcagag
      601 tcgccaacac ggccatgatt tttaagttcg gcgatcgttc catacaccgt ccgcgatact
      661 cctcacccac tagggtatac tcaaaagtcg ccgtgcacgg ttagcaatcc ttcaattgac
      721 ggcgagataa atgattgccc ggcggtttca tagagcgatg ccagcacgag agcgagggcg
      781 gcgactaatt gggcgctatt ttttcgggct ttctttccgg ttgcggcggg ttgtttttgg
      841 gagatagttg cgtagctttg ccgccgcgca actcgcgcag aaaatcgtcc ttggatttga
      901 aggtccggcc ggcgaactgc ccctgatggc aatgatagct taccttgtcc gagccccgat
      961 gacagccgta gccgtcaacc attccggagt ggctcatagc aatattgctg acgcctagca
     1021 aaatcccaag gcctattgaa aatgcaatgc caatccactt gcgcggcgtc atgtcttaga
     1081 tcttgaagat catacatgag gaaccatttt gcgttactcg ggcactccat tgatcagata
     1141 cattgcccga agggcatcgc ttggtttgcc gaagccaccg aaccagcgct cataaatcgg
     1201 cgccacatcg ccagaccgat acagccctgc gagagcccgg ttcaccgcaa gccgaaacgc
     1261 agcatcgttc cggcgtacca taaagccata gggctcatag gaaaataaaa tcggcggtag
     1321 agcaaaaacg ctcgggtcct tcgatgtgac cgcaaggccg atcaaaatac cgcggtccga
     1381 cgcgaacgca tcagctgagc cattctgaag cgctgtgagt ccatcgacgt ggtttttgac
     1441 gggaaccagt ttaactgtaa caaattcttc tttgagaaat ttggcgaaag cggtttccgt
     1501 cgtggtaccc ggaatgatcg cgatccgctt atcggcgaga tccgcgacag cgcgccgggt
     1561 aaaatctttt ttcgtcagca agccgccgcc atccacaaat gtcattagac taaaatcgac
     1621 tcgctcctgc cgagaaaggc ttgccgtggt ggtgccgcat tcaatatcga ctttgcccga
     1681 tgccaccata tcgagccggt tttcggtcgt gaccggcacc cagttcagtt ttagagtagt
     1741 gccaagctga tcctgaattt tgctcgcgat ctgcgtgcag aggtcgatgg aataaccgac
     1801 aggtctctta tccggcccgg ggaaggaaaa tggcggtgca gaagtaaggt agccgagcgt
     1861 gaaggtgttc gagtccttga tttttttgag ggtcccatcc aactcttgac caatcgatgg
     1921 gcgggacaaa cataggctca taagcaatac gccgaaaata atcgtcctct taaacattga
     1981 atcgtcctca aacctcccga agttggatcg tacagccaaa tgctgagcat taagcccggt
     2041 attcgtcttc cctgtatcct cagtgtgcat caatcgaccg cgtccttcac cgtctgttcc
     2101 tacttcacca aaggcacacc cgacgcgata tcatatttga cgacagctcg aatcttgttt
     2161 tctatctgta aataacgcac agctttcctg attggtaatg ccttgctgag cttcggcgca
     2221 tacgtgctct tcagggtggc ttccgctcgg tcgatcgcca gagcttcgtc aatgagcttc
     2281 tttgcctttt catcggtgag cgacttattc cgaaagtcgt ctgcgtagct ctccaaaaga
     2341 ttagccatcc gttgatttat tttttgcagg tctttttggt actgctcata gatcggccaa
     2401 aaattttttg cttccgactc agtaagttcc atattcgtgg caatgactaa ttttttatcg
     2461 gctctgatct tgtcacgcaa aatctgcata ttgtcggctg gcttatcttg cgccaccccc
     2521 aatggggcag taccgaagag catgaacgcc atcgcaacta cgattgtttt ggcgaataaa
     2581 tcgatctgca tcgtttctct ccttcgagct tgattgaaga tttggactac ttgaacggct
     2641 ttgaacagct ttaactgctc agcctaagga tttggattga tcttagtgat cttagtgcct
     2701 tgaatcccga caccccccat caatcctttc tggtcgaaaa tgaatgcgta gatgcctttt
     2761 tgcagcgtcg tggtcgataa atttttgcca aatccagaat ccagcacgac tagactcggc
     2821 ccggttccga gctcgaatcc cgcactatta tcgaggtacc gaagtgaatc ttcatccatg
     2881 aaaaagagca cgtaaccaaa tgcttgcgcc ccggcctgaa atccgtagga cgcggcgagg
     2941 gatcggtaat aagcaacgga tttgccgttt ttgcgcaatg ccccgtcacc gaattgaccg
     3001 gcgatgataa agcccccttt cacgatactg gggaaaacca acacgccgac ggctttatca
     3061 cccaacgcct tcgcaccctg attattcttg taaagcttat ccaaagtcgc tttagcatca
     3121 cggtcaatgg tttttgcgct ggccgccaaa cacatcgtca acatagcggc attgatcgcg
     3181 aggctcatcg cgcaaatcac acaccctaaa cgaatgactg ccattgcatc tctcaatctt
     3241 actcgcccgt agtcaaagct cattcctttt ctccttagcc gccggcatca cgtttcagtt
     3301 gtcagacggt gtcgctctca gcgagcaact cgcccagtac taacgacctg aacactgagc
     3361 ctgaattcct aaccccaatc ggatctaata ttcctcgtga cccgtcctcg accttcccgc
     3421 cgcctactga accagcggta cggtcgcagc aagatcatag cgcaatagaa tgcggaactc
     3481 gttttcgatc tgcaggtaac gcgccgcctt tttggctggc agcgcggcca ggactttcgg
     3541 cgcaaaagcg ctgcgacgtt tgccctcttc ctgctcgaga gcaatccatt catccagtaa
     3601 cttttgagct ttctcatccg tcaatgattt gtttcgataa tctgccgcat agctctccag
     3661 tagcttgcgc aacttctcat tgctgctttg cagatccgtc tggtattcct cgtaaaccgg
     3721 ccagaatttg gcggcctccg attcggtcaa gtccatatat tttgccacag tcaatttttt
     3781 gtccgccttg agcttctcat gaatgatttc cagattgctt gccggcttat cacctgctgg
     3841 cgctttcgtc tgtgcccaag cgtgagtcaa atgcatcagc cccagcagca ttcccacgag
     3901 cacaaactct ggaaccgttc gtcttgtcat gaattctcct ttcgatcgca gtattcagcg
     3961 ttacgtccga tcactgctgt tcacgcacca tgcgaatttg atgatatgca actgaatagc
     4021 agacaccgcc tacgagccgg tattggactt tggtccaata agagcaaatc gcatgtagaa
     4081 tgagctggct actcgcatca agacaataga cgaaaagccg cattggcgca ctgcggtgcg
     4141 tcatcgatct aaggaccatc gcgtaattcg ccgtcgtgaa tatcgtcctc gatccaagcc
     4201 atgagtagct tatccgcaat ggccaatatc accggaccga caaaaatgcc gatcaatccg
     4261 aaggcgataa gaccacccac cacaccggcg aaaacgagaa gcaacggtaa gtctgcacct
     4321 ttcctgatca gaatcgggcg caaaaaatta tccatcgtgc cggcaatcag catccagacc
     4381 accatgaatg ttccccaccc cgattgaccg gtccagtaaa gccacgccac gaccccccca
     4441 agcacgggaa tgactccaat ctgcgcgatg gataaaagca gcattgcacc cgtgagcgcc
     4501 gcggcgaagg gaactccagc aatgatcaaa ccgatgccgg aaaaaagcga ctgtacgatg
     4561 gccgtcacaa cgaccccgag cgcgacaccg cgaatagtct ggccggctag atagatcgcg
     4621 ttttcaccgc ctgaccccgc caattttcgg ccgaaacgaa gcagccgctc cgttgcacgc
     4681 tctccgctcg catacatggt ggccgccaga attattgtca gtaaaaattc caggaataat
     4741 acgccgacgc ttcccaattg agcagcgaac cacttgatca caccacccgc gtatggcgcc
     4801 actctgacgg tggcatcctg aattccgaca atggcgactc gctcccacaa ctccgcggct
     4861 ttcgcgccaa cgaggggcag acttgccagc caagtcggcg gcggaggtag cataaaggaa
     4921 gccaaatctt tcacccagat cgcaatatca tcgaggttcg acgcaatcgc gccgatggca
     4981 aacgtcagcg gaactactag gacacagacc agcaaaagcg tcatcacgat taccgctaac
     5041 acgcgctttt tccccaacca tttttgaagc gaaatcatga aaggccaggt cgccaccacc
     5101 actgtggtcg cccagacgat cgcgcctaaa aacggccgta atatccagat tgaggacccg
     5161 atcaaagcga gaataaagat aacggcgagc aggttgcgtg tgatatcgcg ttgtatgtca
     5221 gccacaagca aaccttccca agttgttgct cacacctgaa atgatgcaat ggccggcaaa
     5281 gtcgatgtcg gataacgaaa ctgttatctt acccgaccgg gatcgtgatg agcggcgcgt
     5341 ttggcttgcc ctccgtttca gtgagcgtga agagaaacat gatgaacgaa aagagccgct
     5401 tggaagcaaa atctttgtcg tcgatccaat accattcgcg gccgtacgga accgcggcga
     5461 acgtgtcgcc gggagcttga gaagagctgg aaatccttat gagcggcacc acaggcgtgc
     5521 cgttcacgat ttccggcgcc ggagttgcaa acgtccgccg ttctgtcgcg cttgcggctg
     5581 gaacttctat ataagaagaa agatcgagca gaatttcgag catggaccgg gtgagcatgg
     5641 cgatctcttt gtcgttgatc gggactgaac cgtagacgac gttgagttct tcggttttag
     5701 gatcaagacc caatagctta cgcactgcgg ctcgatccgc atcgatggct gggttgggtt
     5761 ttttactaaa taccagcatg gtcgtcgtct ggtcaccggt ttttcgtacc cgtagaccga
     5821 gatccccgga ctgctggata ttacgcaatc tttccaccag ctcataaaac tccgtgtcag
     5881 cttgacgcgc tctcgctccg ctaccgaaac ggctattgat gccgttgatg ctatgcacgc
     5941 acagccgcag aaccaaatct actggatacc cgccctgaag cagattgagg atcgccgccg
     6001 gcggaatcgg cgtcatgaga ttgcgggcga atttctcccc gctcaacggc gcatacgtaa
     6061 tggtaggcct ctcgacatac cttcccgatc cgagcaagtt ataaaccgct gtatacggcc
     6121 acggaagctg ggaattctgc gcccagcttc cggagaaacc aagagagccc tcgactccgt
     6181 attgattaat caccgaagcc acatccagaa acacaggcgc atcgccatat cggagcttga
     6241 ccaggttcag caacatctga gatttccacg aatccgagat cgctgaaatg taatcaaagc
     6301 gatcgcgtgc cacggtgccg ggaccgacac tggcgcagcc gccgagcgtg agcagcaccc
     6361 agaccatgat cgaaggcgtt ttccgctgca ttttttctcc tcacttgacc atcaagcatg
     6421 cgccgacggt cgccgccaaa tccgaccgtc cagccagtag tggcagaaga gtccgccggc
     6481 gccgaagggt gctagtgccg gaaagttgag ccaactcccg aaaaccgctc cgacgaagcc
     6541 aatcaccggc cagaaaagga acgacccgat gaagcttact tcggtgcgga tcccgcgatc
     6601 agctcggcac ggaccgggcg agtaaacatc ctggaccgcc gactgaacag ccaaggattg
     6661 tgaattcgtt ctgcgccggg ccatcgcaca ggtaaacgct agatattgga cctcatggtg
     6721 tactgcgatc acaaacaaat aggaggcatt ggccgccccc aacacatagc cgctgccgaa
     6781 ggcaacggcg tgcgcgagca ccacccacca ctcgccggga tgaactgcat taaggatgtt
     6841 tccggcacta ggtcccacgg cgcgagatcg cgatgcgctc cagatcaggt aagcgatcag
     6901 ccaaatgaca gcagccaagc cgattatcca agtgactcct gctcccacac tcaacgcgag
     6961 atatggccct ccatcgtact ccgtcggggt taaatgagta tatgcgaggg tagcagcggc
     7021 catcggcaga taaatcgcgc cg
//
LOCUS       DASULZ010000054         3418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_5891, whole genome shotgun sequence.
ACCESSION   DASULZ010000054 DASULZ010000000
VERSION     DASULZ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5891"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VFY96_01085"
     CDS             complement(<1..293)
                     /locus_tag="VFY96_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HEX5605076.1"
                     /translation="MTTDYIWLEPILIFFLILANGFFAASEIAMIAMRKSRIDALVER
                     GVRSAAVVARLKADPDRFLATVQIGVTVISTFASAIGGAAASGYLKPQIAALP"
     gene            498..2747
                     /locus_tag="VFY96_01090"
     CDS             498..2747
                     /locus_tag="VFY96_01090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEX5605077.1"
                     /translation="MAEKQNETTSIQGTVGRITYQHPETHYTVARLDEQAGGGITIVG
                     SIFPVSEGEEIKVTGAWKRHPRYGLQFQVDHWIKVDPATCEGIEKYLGSGLIKGIGPT
                     YAKRLVAAFGLETLQILSEQPLRVMDVEGIGEVRARRIIQAWQQQRGMQDVMVFLQGH
                     GVGASLALRIYRVFGADTITQVRENPYALAQQVHGIGFLLADRLANHIGIRGDFPLRV
                     QAGVLHVLREMAERGHCFAAFTALKQNVAALLNVDEDPVEAAIGKLAARGDLVLHESE
                     NADAQVYLDELYQAERRIVRALQNLLSTPSTLEGTPLLTHGEWPNSVSRGKVDLVSPE
                     LFAPSAVTLDEEQMRAAEQALKEKVLVITGGPGTGKTTLLTALLAVLNRAKLRFALAA
                     PTGRAAKRICESTGEDALTIHRLLEYNPRDGGFQRNEENPLQADFIIVDETSMVDLPL
                     MDHLLRAIDPRSHLILLGDVDQLPSVGPGSVLRDFIDSGVVPKFVLRRIFRQDRESLI
                     VANAHRILQGKSLWLGENGDNRDFAFIPHESEDDILMTLKTLVREQIPLSFQLNPNEV
                     VQSIQVLTPMHRGMLGTVNLNKELQALLNSNGAALERAGSRLRAGDKVMQLRNNYEKG
                     VFNGDLGRIASIDPEDGSAKIDFIDRVVEYDSDELDEITLAYATSVHKSQGSEYPVVV
                     IPLHTSHYVMLHRSILYTAVTRGKKLVVIVGSRKALAMAIRNVRVERRNTGLTEKLRT
                     LIPHQPQFA"
     gene            complement(2800..>3418)
                     /locus_tag="VFY96_01095"
     CDS             complement(2800..>3418)
                     /locus_tag="VFY96_01095"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arylmalonate decarboxylase"
                     /protein_id="HEX5605078.1"
                     /translation="GVGVHVTRLRMTGKFRKPLSELKRPLTEAAQAISDVKPGVIVFH
                     CTANSMESGRAHEQALIEIIEQASGCPTITTAQAITQAFDRSEIKKLVLVSPYVQSTN
                     QLEVNYLSETGYQVLHEVGLGLETHAYAAVTPQEWQAVVKENSRADADGYFLSCTNTR
                     MIEAITDLERDLGKPVINSNQATLWACLKKLGIQHTDPRLGSLFH"
BASE COUNT          749 a          932 c         1003 g          734 t
ORIGIN      
        1 aacggcaacg cggcaatctg tggtttcaga taaccgctcg ccgccgcgcc gccaatggcc
       61 gacgcgaagg tgctgatcac cgtcacgccg atttgcactg tggctaagaa gcgatcggga
      121 tcggccttga gacgcgccac cacggcagcc gatcggaccc cgcgctcgac cagagcatcg
      181 atgcggctct tacgcatggc gatcatggcg atttctgatg ccgcgaaaaa gccattggcg
      241 aggatcagga aaaaaatcag aatcggttcg agccaaatat agtctgtggt catgtcaaag
      301 tctcctggtt tttgccgctc ggaaaatcgg caaaagcgct ggcactgtcg agggatttag
      361 caatggaatc ggcggtggaa cgccatgatt ggaggcaggt ttcggcgatc ggtttgtcgg
      421 ggattttagc gccatgccgg caaaaggcaa ggggaagcgt ttaccggtca aatcctttgg
      481 cgtaggatac ccctcgcatg gcggagaagc agaacgaaac gacctccatc caggggaccg
      541 tcgggcgcat cacttatcaa cacccggaaa cacactacac ggtggcgcgc ttggacgagc
      601 aagccggcgg cggaatcacc atcgtcggga gcatttttcc cgtttccgaa ggtgaagaga
      661 ttaaagtgac aggcgcatgg aagcgccatc ctcgctacgg cttgcaattt caggtcgatc
      721 actggatcaa ggtcgatccg gcgacctgcg aaggaatcga gaaatatctc ggctcggggt
      781 tgatcaaagg cataggcccg acatatgcga aacggctcgt cgccgctttt ggactagaga
      841 cgctccaaat tttgtcagag cagccgctgc gcgtgatgga tgtcgagggc atcggcgaag
      901 tgcgtgcgcg acggatcatt caggcgtggc aacaacagcg cggtatgcag gatgtcatgg
      961 tttttcttca aggtcacgga gtgggggctt cgctggcgct aagaatctac cgcgtatttg
     1021 gcgcggacac gatcacccaa gttcgtgaga acccctacgc gttggctcag caggtgcacg
     1081 gcatcggatt cctgttggct gaccgtttgg ccaatcacat cggtatccga ggcgactttc
     1141 ccttgcgggt acaagctgga gttctgcatg tgctgaggga gatggcggaa cgcggccatt
     1201 gcttcgctgc gtttacagcg ctcaagcaaa atgtcgcggc actgttgaat gtggatgagg
     1261 atcccgtgga ggccgccatc ggcaagctcg cagccagagg tgatttggtg ctacacgaga
     1321 gcgaaaacgc agatgcccaa gtttatttgg acgagcttta ccaagcagag cgccggattg
     1381 ttcgcgcgtt gcaaaatctt ttatcgacgc cctccactct ggagggcaca ccccttctca
     1441 cccacggcga atggccgaac agcgtctcgc ggggaaaggt cgatctggtc agtcccgaat
     1501 tgtttgcgcc ctcggcggtc actctcgacg aggagcagat gcgcgccgcc gagcaggcgc
     1561 tcaaagaaaa ggttctggtg atcaccggcg gtccgggcac cgggaaaacg accctcttga
     1621 ccgcgcttct ggcagtctta aatcgggcca agctgcgctt tgcactggct gctcccaccg
     1681 gccgagccgc caaacggata tgcgaaagta ccggagaaga cgcactgaca atccaccgat
     1741 tactcgagta caatccgcgc gacggcggct ttcagcgcaa cgaagagaat ccactgcagg
     1801 ccgatttcat catcgtcgac gaaacctcca tggtcgattt gccattgatg gatcacttgc
     1861 tgcgcgccat cgacccgcgc agccatctga ttcttctcgg cgatgtcgat cagctgccct
     1921 ccgttggacc gggcagcgtg ctgcgcgact ttatcgattc cggcgtcgtt ccgaaatttg
     1981 tcttgcggcg aatttttcgc caggaccggg aaagtttgat cgttgccaat gcgcatcgga
     2041 tcctgcaagg aaaatcactc tggttaggtg aaaacggcga taaccgcgac tttgcattca
     2101 ttccgcatga atccgaggat gacattctca tgacgctgaa aacgctggtg cgtgagcaga
     2161 ttccgctatc gttccagctg aatccaaacg aggtagttca gtcgattcag gtgctgacgc
     2221 cgatgcatcg cggcatgctc gggacggtca atctgaacaa ggagctgcag gcgctgctga
     2281 atagtaacgg tgccgctttg gagcgcgctg gatcgcgatt acgcgccggc gataaggtga
     2341 tgcagttgcg gaataattac gaaaagggcg tgttcaatgg cgatctggga cgaattgcca
     2401 gcatcgatcc ggaggatggc agcgccaaaa tcgattttat cgatcgcgtc gtcgaatacg
     2461 attccgacga gctcgatgaa atcactttag cctatgcgac gtccgtgcat aaaagccaag
     2521 gcagcgaata tcccgtggtg gtgattcccc tgcacacctc gcattatgtc atgctgcatc
     2581 gaagcatcct ttacaccgcc gtgacgcgcg gcaaaaaact agtcgtgatc gtcgggagcc
     2641 gtaaagcttt ggcaatggcg atccgcaatg ttcgcgtgga gaggcgtaat accggtttaa
     2701 ccgaaaagct ccgcacactc atcccgcatc agccgcaatt tgcttaagcg taaacgagcg
     2761 cgcgtggtag caatgaacca gggttcgggt gacgacggcc tagtggaata ggctgccaag
     2821 gcgcgggtcg gtatgctgga tgccgagttt cttgagacaa gcccagagcg tcgcttgatt
     2881 gctgttgatg accggtttgc ccaaatcccg ttccaagtcc gtaatggctt cgatcatccg
     2941 cgtattcgtg cagctcagaa aatagccgtc ggcatcggcc cggctatttt ctttgacgac
     3001 agcttgccat tcttgaggcg tcaccgccgc ataggcgtga gtttcaagac cgagccccac
     3061 ctcatgcagc acctggtacc cggtttcgct gagatagttc acttcgagct gattcgttga
     3121 ttgtacatag gggctcacga gcacgagctt tttgatttcc gatcgatcga aagcctgcgt
     3181 gatcgcctgg gctgtcgtga tggtcggaca gccgctcgcc tgttcgatga tttcaatcag
     3241 cgcctgttca tgggctcggc cgctctccat ggagttggcc gtgcagtgaa atacgatcac
     3301 tccaggtttc acgtccgaaa tcgcctgcgc cgcttcggtc agcggtcttt tgagctcgct
     3361 cagcggtttg cgaaatttgc cggtcatgcg taaccgagtg acatgcacgc cgacaccg
//
LOCUS       DASULZ010000055         4141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1426370, whole genome shotgun sequence.
ACCESSION   DASULZ010000055 DASULZ010000000
VERSION     DASULZ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426370"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..731
                     /locus_tag="VFY96_01100"
     CDS             240..731
                     /locus_tag="VFY96_01100"
                     /inference="COORDINATES: protein motif:HMM:NF028512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605079.1"
                     /translation="MLRHQMDETALLLTTSLSDAAATYLARKDLLRLNTTVTKYALLS
                     RVVYVVIRDDEDKVIAHSPKSLPSGLEHDLGFDHGRREVIRREITLEGQTVYETSAPI
                     LEGQLGRAHIGISAAAVQSEIDRALFLFVWPIAVGILAGAIILTVGSRLCWARSKRVG
                     QLG"
     gene            complement(1441..1695)
                     /gene="grxC"
                     /locus_tag="VFY96_01105"
     CDS             complement(1441..1695)
                     /gene="grxC"
                     /locus_tag="VFY96_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010652721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEX5605080.1"
                     /translation="MAQVRIYTTNYCPYCRAAKALLRRKAVQFEEIDVTDDPQLRAEM
                     EKLSRRMTVPQIFINGEPIGGYDDAQELDASGELDRLLGG"
     gene            1770..2366
                     /gene="coaE"
                     /locus_tag="VFY96_01110"
     CDS             1770..2366
                     /gene="coaE"
                     /locus_tag="VFY96_01110"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019534595.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX5605081.1"
                     /translation="MKLVGLTGGIASGKSTVAKNLQRLGAAIVNADDLARQVVEPGRE
                     AWKEIVALFGPDVLQTDQNLDRQKLRTLIFNQPEARKRLESIIHPRVRALAEERIRQY
                     AAAGYPIVVYEVPLLFEGNLQEWLRPVILVACNIETQTARLQKRDHLTAADAEKHIAA
                     QMSLAEKRRLADYVIENNGSLEDLERQTREVLEELKAT"
     gene            complement(2359..2919)
                     /gene="efp"
                     /locus_tag="VFY96_01115"
     CDS             complement(2359..2919)
                     /gene="efp"
                     /locus_tag="VFY96_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX5605082.1"
                     /translation="MVQATQLRPGMIIIHGGDLFRVTAVHHLTPGNKRGFMQTKMKNL
                     RTGIGTEYKFRSEDRVDQAILDNRQMQYLYAEGDLHTFMDLENYEQITLAKEDIGDLL
                     SFLLPNSIVGIEFHEGRPVGINPPSTVDLEVVDTEPSMKGATASASYKPAKLETGVTV
                     QVPPFVQVGDKVRVDPTDGSYLERVK"
     gene            complement(2987..>4141)
                     /locus_tag="VFY96_01120"
     CDS             complement(2987..>4141)
                     /locus_tag="VFY96_01120"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX5605083.1"
                     /translation="RRPDRALIVSVPTRMDDGGVKVFTGYRVQHNDVLGPFKGGIRYH
                     PAVNLGEVSALAMWMTWKCSLVGLPLGGAKGGIACDPAELSRHELQSMTRRFTAEIIN
                     VIGPEVDVPAPDMGTGEQVMAWIMDTYSQHKGHAVPEIVTGKPVAIGGTLGRREATGR
                     GVVYMILEAAKHLNIDLGQCTAVVQGFGNVGSVAVKELANIGVKLIAVSDRTGGFVDV
                     KGLPVEKLLELADKNHSLEGCPYGDKISNEQLLELKCDVLVPAALEMQITKANAAKIQ
                     CRILAEGANGPTTPDADAILRENGIFLIPDILANAGGVVVSYFEWVQDLQNFFWNEVE
                     VNSKLREILVKSFHEVLRMSEKHHVDMRLAALMIGIDRVARAMLWRGLYA"
BASE COUNT          928 a         1157 c         1097 g          959 t
ORIGIN      
        1 cgagcctaag aaacgagtgg cttgttgtgc ctgatacgaa agttgacaca ctagcagcgg
       61 caaattaggg aatctggtca tcttatgagc gtagtgtatg atgcgctaac aaaagcagcg
      121 gcggaaaaga cgcgagcaac cgacgcgctc aatacgtggt ctagcgtgaa tctgcgctgg
      181 atgatcatta tcacatttac tcttctgttt tggcaactgt aaaccagctt gtggcgcggg
      241 tgttacggca tcaaatggat gagactgcgt tattgttgac aactagtctc agtgatgccg
      301 cggcaacgta cttagccagg aaagatttgc tgcgcttaaa tacaacagtt acaaaatatg
      361 ctttattgag cagggttgtt tacgtcgtta ttagagacga cgaggacaaa gtgattgctc
      421 atagtccgaa aagtcttcca tcaggtcttg agcatgacct tggtttcgat catggccggc
      481 gcgaagtaat tcggcgggag atcacattag agggacagac cgtatatgaa acgagtgcgc
      541 caattcttga ggggcaactt gggagagcgc atattgggat ttcggcagct gcagtacaat
      601 cggaaattga ccgggcgctg ttcctctttg tctggccgat tgcggtgggg attttagccg
      661 gcgctataat actgacggtc gggtcacggc tttgttgggc acgttctaag agagtcggac
      721 agcttggcta gtccgaaaca tttacgttgc ggaatagaga gaccgagggt gcgagcggct
      781 tgttggacgg aatagccaga taagtcgcaa ggattttctt gctgttgatc gtcctgccaa
      841 ttatcgtttg cgcaataagt tactgaccag aaaacccgcg gtgcactcgc caacgggccc
      901 ggctccgata agaacagttt gacgagttcc cgccaatcat aaagccaaca gacatgacat
      961 tgtcgccagt cgcgaaactc cccgcgtgga tacattaatt agctgagcca acctcaggtg
     1021 aggcagggcc gcaattaaga ccagcaccta gccaattagg tgtctgtcgc ccgtactatt
     1081 gacatgcact gatgcggccc gccagtgtaa ctcaacagaa tctgggagaa gcttttttgt
     1141 ttttcgccgt gtccggctac ctcccctaag ccgaaacacc ccccggcggg cgtgccaagt
     1201 cgccgtccgc aaatcatgcg aaaaaacgtt ccagaaatgt tccgggtttg tgccaatacc
     1261 aggtgacaaa ccatagcacg ctgtgacatg tcaacgctga caagcccttg attttcttac
     1321 cttccgctcg ttcctatcac tccttgtttt tgcggttttg gcctcgatgc agcggttcga
     1381 atccgctcgc cgcctccagt tgagtcacgg gtctgctcac gcgccgcttg atcgctggca
     1441 tcagcctcct aaaagccgat ccaactcgcc gctggcgtcc agttcttgcg catcgtcata
     1501 gccaccgatc ggctcgccat tgatgaagat ctgcggcacc gtcatacgcc gcgaaagctt
     1561 ctccatttcg gcccggagtt gtggatcatc ggtgacgtcg atctcctcga actggacagc
     1621 cttcctccgc agcagagctt tggccgctcg acaataggga caatagtttg tcgtgtagat
     1681 ccgtacctga gccatggcaa atagctcccc tttcatctgt taatcgcgca ttgcttgaaa
     1741 ccactctttt attttgcgaa gagtgttgcg tgaaattggt cgggctaacc ggcggcattg
     1801 cctcaggtaa aagcacagtg gcgaaaaatc tccagcgttt aggcgccgcc attgtcaatg
     1861 cggacgattt ggcccgccag gtggtcgaac ccggacgcga ggcatggaaa gaaatcgtcg
     1921 cgctcttcgg accggatgtg ttgcaaaccg atcaaaacct cgatcgccaa aaactgcgca
     1981 cgttgatctt taatcagcct gaggccagaa aacggctgga atcgatcatt catccacgcg
     2041 tgcgcgccct cgctgaagaa agaatacggc agtatgccgc ggcgggctac cccatagtcg
     2101 tttatgaagt gccgctcctc ttcgaaggca acctgcagga gtggctgcgg ccggtgatct
     2161 tggtcgcttg caatatcgaa acccaaacgg ctcgtttgca gaaacgcgat cacctcacgg
     2221 ccgctgacgc tgagaagcat atcgcggccc agatgagctt ggcagaaaaa cggcgactcg
     2281 ccgattatgt catcgaaaac aacggcagtt tggaagatct cgaacggcaa acgcgggaag
     2341 tcctggaaga actcaaagct acttgacgcg ttcgagatat gagccgtcgg tcggatcgac
     2401 gcgcacttta tccccgactt ggacaaacgg cggcacttgc accgtcactc cggtttcgag
     2461 cttggccggt ttgtaggacg cgctcgcagt tgcgcctttc attgaaggct ccgtgtcgac
     2521 aacttccagg tccacggtgg acggtggatt gattccaacc ggcctgccct cgtgaaattc
     2581 gatgccgacg atcgaattgg gcaacaggaa tgataacaga tcgccgatgt cttcttttgc
     2641 taacgtaatc tgctcgtaat tttccagatc catgaaagta tgcaagtccc cttctgcata
     2701 aaggtactgc atctggcgat tgtcaagaat cgcctgatcg acgcgatctt ccgagcgaaa
     2761 cttgtactcc gtcccgatcc ccgttcgcaa atttttcatc ttggtctgca tgaagccgcg
     2821 cttattcccg ggggtcagat gatgcacggc cgttacgcgg aacaggtcgc ctccatggat
     2881 gatgatcatt ccgggccgaa gttgcgtcgc ttgaaccatt gactaccgtc tctctttcat
     2941 ttggatgccc cttggttaac agtttctggt tcccgcttca aatcgttcat gcgtacagcc
     3001 cacgccaaag catcgcgcga gctacccgat cgatgccgat catcagcgcc gcgagtcgca
     3061 tgtcgacatg atgcttttcg ctcatccgga gcacctcatg aaatgatttg accagaatct
     3121 cccgcagctt gctgttgacc tcaacttcat tccagaagaa attttgcaga tcctggaccc
     3181 attcgaaata agaaaccacc accccgccgg cgttcgccag gatgtcggga atcaggaata
     3241 tcccgttctc gcgcaaaatc gcatcggcgt cgggcgtcgt tggcccgttc gcaccctcag
     3301 cgaggatgcg gcactgaatc ttcgccgcat tggctttcgt gatctgcatc tcgagggcgg
     3361 cgggcaccag cacgtcgcac ttgagctcca ataattgttc gttgctgatc ttgtcgccgt
     3421 aggggcagcc ttccaaactg tggtttttgt cggcaagctc caacagtttt tccaccggca
     3481 atcctttcac atcgacaaaa ccgccggtac ggtcggaaac ggcaatcagt tttacgccga
     3541 tgttggcaag ctccttgacg gccaccgaac cgacattgcc gaagccctgg accaccgccg
     3601 tgcactggcc gagatcgatg tttagatgtt tggcggcttc aagaatcatg tatacaacac
     3661 cgcggccggt cgcctcgcgc cgcccgagag tgccgccgat ggccacgggc tttcccgtca
     3721 cgatctcggg caccgcatgt cctttgtgtt gggaataggt gtccatgatc catgccatca
     3781 cctgctcgcc agtgcccata tccggcgccg gaacgtcgac ctcgggaccg atcacgttga
     3841 tgatttcggc agtgaagcgg cgggtcatcg attgcagctc gtggcgcgac agctccgccg
     3901 gatcgcaggc gattccgccc ttcgctcctc ccagaggcag accgaccagt gaacatttcc
     3961 atgtcatcca catcgccagg gcggaaactt caccgagatt gactgcggga tgatagcgga
     4021 tgccaccttt gaacggaccc aagacatcat tgtgctgtac ccgatagccg gtaaaaactt
     4081 tgactccgcc gtcatccatc cgcgtcggca cactgacgat cagcgcgcga tccggacggc
     4141 g
//
LOCUS       DASULZ010000056         6184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_513412, whole genome shotgun sequence.
ACCESSION   DASULZ010000056 DASULZ010000000
VERSION     DASULZ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6184
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513412"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /gene="lipA"
                     /locus_tag="VFY96_01125"
     CDS             complement(<1..795)
                     /gene="lipA"
                     /locus_tag="VFY96_01125"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX5605084.1"
                     /translation="MPRRHPDWIKVKAPGNPNYLRLKRLVREKNLHTVCEEARCPNIG
                     ECWGNKTATFLILGDTCTRGCRFCAIDKGKPLALDPEEPRNVALVVKDLALDHIVVTS
                     VNRDDLPDGGAAHFAQTVFWIKHFNPGIRVELLVPDFAGNLQSLEIVVDSGIEILNHN
                     IETVARLYGKVRPGHSYEGSLDLLRAAKKHRPEILTKSGFMLGVGETAQEVFATLQDL
                     RDNQVDIVTIGQYLQPSTKQLRVDRYVTPDEFHDFKAKAELLGFRHV"
     gene            complement(799..1497)
                     /gene="lipB"
                     /locus_tag="VFY96_01130"
     CDS             complement(799..1497)
                     /gene="lipB"
                     /locus_tag="VFY96_01130"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX5605085.1"
                     /translation="MAALLYQNLGTLDFFSALELQEKFVARKQRQSSPDILLFVEHSH
                     VYTLGRGGNQVNVLAPEDVPVIRTSRGGDVTYHGPGQLVVYPIIDLRSKLRKDVHCYV
                     RNLELAAIQTLRDFGLSGARRPPFTGIWIGDKKIAAIGVAVRRCITFHGLALNVNPDL
                     AYFKRIVPCGLSWADVTSMSRELGTNQDFSAVRDGFLQNFAEIFGYDRPKQVFSERHS
                     GDWNGLNFLNGLNN"
     gene            1628..1921
                     /locus_tag="VFY96_01135"
     CDS             1628..1921
                     /locus_tag="VFY96_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605086.1"
                     /translation="MLIALAQKNSCLALPLRNHVISPKFQRTTRKVFAPFQFDPEATK
                     DIDENDWPGVILLKVQMNAAFDGRPEENRASNESFTPGDIVELLGRSVQTMDA"
     gene            1988..2545
                     /locus_tag="VFY96_01140"
     CDS             1988..2545
                     /locus_tag="VFY96_01140"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX5605087.1"
                     /translation="MKLSVIKPIDAEYPQRLLERLGERAPKSLTTAGDIALLFQPKTA
                     LFCSARCASDKMLSACEAVRKLRDNGVTVISGFHSPVEKKSLQILLKGNQPIIICLAR
                     ALEKIRVPSGWREPLESGRMLLLSRFDKSRRADKDTARRRNELVAALADEALIIYAEQ
                     GGGIEKISAMIDQWLIPKIDLAKGL"
     gene            2688..2984
                     /locus_tag="VFY96_01145"
     CDS             2688..2984
                     /locus_tag="VFY96_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605088.1"
                     /translation="MMKIDIDQLSEEELIDLNHRIVERLRFLTQMRAHSEMLDSKIGE
                     RVTFHPNGHPQLFGILTRYNRKTVTVITDTGQQWNVAPSVLRRADARATAGGGP"
     gene            3176..4720
                     /locus_tag="VFY96_01150"
     CDS             3176..4720
                     /locus_tag="VFY96_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEX5605089.1"
                     /translation="MPQSSFEPAWFVRKDLDGFFGLMIDNLIQLILIVSLCRELIHLS
                     NEYIFGRILPGAAISILVGNFFYAWQARRLARATGREDVTALPYGINTVSLFAFIFFI
                     MLPTYLETKDPVWAWKIGLVACFLNGVIEIIGAFVAETVRRVTPRAALLSALAGIAIT
                     FISMDFTFKIFARPLVALLPMAIIFVAYFSRQRLPLGLPGGMLAIAIGTVLGWVLGTM
                     NGSAIAASYDFALPKYSGDSLWQAITDPAFLSYFSVILPMGIFNVVGSLQNIESAEAA
                     GDRFRTFPSLMANGIGSVVAALFGSVFPTTIYIGHPGWKRLGARSGYSALNGIFISVL
                     CLTGTIQTVLTVIPLEAGIGILLYIGIIIVAQSFQETPKEHAPAVALGIVPALAGWGL
                     LMVETGLRAGGKSLYAVGLSNFDQESLAIRGMISLERGFIFTSMILAAIGVALIERRF
                     AQAGAWAAAAALLSAFGIIHAYDLTSSGVTSRFGWMAAPEFCAAYLLLAGLFFLIAML
                     ARNGAR"
     gene            complement(4724..5374)
                     /locus_tag="VFY96_01155"
     CDS             complement(4724..5374)
                     /locus_tag="VFY96_01155"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX5605090.1"
                     /translation="MQILLVRHGATDWNLQGRCQGSSDRHLSEVGVRQAEHLAALLRA
                     EEIHAIYSSHLRRALQTADYISQPHKLPVLIEQKICELDHGALEGLTFNQIKENYAEF
                     LVRWRSEPAEIRVPGGERLADVAERAWNGLNQIVQRHSEAERILVVSHNFPILGIVCR
                     ITGTHLNAYRSFHIDPCGVTRLAYDAGRWNLTAVNSREYNPTPLPYAENGPTSASK"
     gene            complement(5391..>6184)
                     /locus_tag="VFY96_01160"
     CDS             complement(5391..>6184)
                     /locus_tag="VFY96_01160"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX5605091.1"
                     /translation="ESLKEATPEITKILKAEKAKSEAAKMADRDREKALSGTELSKLA
                     QESGVKITVSNWLAAGETLPEIGENQEFYKSAFALAPKDVSPVIEGKNGYYLIRLKAR
                     QEPATPPLESVRDQIEKNLKESKAYELASQKGNSLLEQLKKEKEISKLAQTGDLKIEE
                     TGWFPRSSQQLPKIGELAELKGGPISLSAQKPIPERLYTQKDAVYVLAFKDSQPVDMA
                     QFDKDKAALMKQAQAEARQRVLVKFLENLKAKAKVKINNAFLEES"
BASE COUNT         1306 a         1608 c         1714 g         1556 t
ORIGIN      
        1 gacatggcga aaacctaaaa gctctgcctt cgctttgaag tcgtggaatt cgtccggtgt
       61 cacatagcgg tcaacgcgaa gctgcttggt agatggctgc aaatactgac cgatggtgac
      121 aatgtctacc tggttgtcgc gcaaatcttg caatgtggcg aaaacttcct gggccgtttc
      181 gccaacgccg agcatgaaac cggatttggt gaggatctcc ggacggtgct ttttagccgc
      241 cctgaggagg tccagcgagc cttcatagct atgtcccgga cggaccttgc cgtatagacg
      301 cgccacggtt tcaatattgt ggttgagaat ttcgataccg gagtcgacga cgatttcaag
      361 cgactgcaag ttgccggcaa aatcgggaac gagcaattct accctgatcc ccggattgaa
      421 atgcttgatc cagaataccg tctgggcgaa atgggctgcg ccgccgtcag gcaggtcatc
      481 gcggttcacc gaggtaacga cgatgtggtc gagagcgagg tcttttacca ccaaagcgac
      541 attgcgcggt tcttccggat ccagggcgag cggtttacct ttgtcgatgg cacaaaatcg
      601 acagccgcgc gtacaggtat cacccagaat caagaatgtc gcggttttgt tgccccagca
      661 ctcgccaata tttggacagc gcgcctcttc acaaaccgtg tgcaggtttt tttcacgcac
      721 gaggcgcttc agccggaggt agttgggatt acccggcgct ttgactttga tccagtcggg
      781 atgtcggcgg ggcatagatt aattgttcaa gccgttcaaa aagttcaaac cgttccaatc
      841 gcccgagtgg cgctcggaga aaacttgttt agggcgatcg tagccgaaaa tctcggcgaa
      901 gttctgaaga aaaccatctc gaactgccga aaaatcctga ttggtgccga gctcgcgcga
      961 catcgacgtg acgtcggccc aactgaggcc gcaggggacg atgcgtttga aataagccaa
     1021 atccggattt acgttcagcg ccaaaccgtg aaaagtgata cacctccgca ccgcaacacc
     1081 gatcgccgcg atctttttat cgccgatcca aatccctgta aagggagggc gccgggcgcc
     1141 actcaaaccg aagtctcgca aggtctggat cgccgccaat tcgagatttc tgacgtagca
     1201 gtgcacgtct tttctcagct tcgagcgcag atcgatgatt ggataaacga ccagttgccc
     1261 gggcccgtga taggtcacat cgccgccacg gctcgtgcga atgaccggaa cgtcttcggg
     1321 cgcgagcacg ttgacttggt tgccgccgcg gcccagcgtg tagacatgag aatgttcgac
     1381 aaagaggaga atatcgggcg aggactggcg ttgcttgcgc gcaacgaact tttcttgtag
     1441 ctccagggcg gaaaaaaaat ctagagttcc aagattttgg tataggagag cggccattag
     1501 atgattctag tcttaacctc agcactctga cgtttcaagc taggccggta tcggacgcta
     1561 cattgttaag atgcgtaacg ccgaaaaagg gtaacccctt cagtttggtc caagtgcggt
     1621 tgataggatg ttgatcgcgc ttgcccaaaa aaattcttgc cttgccctgc cattgcggaa
     1681 tcacgtaata tcaccgaaat tccaacgcac aactcggaaa gtcttcgcgc cgtttcagtt
     1741 cgatcccgaa gcgaccaaag atatcgacga gaacgattgg ccgggagtga tcctgctcaa
     1801 agtgcagatg aacgcggctt ttgacggaag acctgaagaa aaccgcgcga gtaacgagag
     1861 cttcacgccg ggcgatattg tcgagttact cgggcgttca gtgcaaacga tggatgcata
     1921 aattggaatc cttcaggcgg cgcactatgg gcttacaaaa tacggaattg gatattatta
     1981 ccagccggtg aaattgagtg taatcaagcc gatcgatgct gaatatcccc aacgtctgct
     2041 cgagcggttg ggtgaacgtg cgccaaaatc acttaccact gctggcgata ttgcgctgct
     2101 atttcagcca aagaccgcgc tgttttgctc cgcccgctgc gcttccgaca aaatgctctc
     2161 cgcctgcgag gcagtccgga aattacgcga taatggagtt acagtaatta gcggttttca
     2221 ttctcccgtt gagaagaaaa gtttgcagat tctgctgaaa ggaaatcagc ccatcatcat
     2281 ctgtttggcg cgcgccctcg agaaaatccg tgttccgtcg ggttggcggg agcctttgga
     2341 atccggtcga atgctcctgc tttcacggtt cgacaaatcc cgccgtgctg acaaagacac
     2401 agctcgccgc cgcaatgaac tcgtggcagc tttggccgac gaagccctga ttatttacgc
     2461 cgagcaaggc ggaggtattg aaaaaatttc ggcaatgatt gaccaatggc ttattccgaa
     2521 gattgatctc gcgaaagggc tctgaccgca agacgcgacg ggctgctatc ggagttggaa
     2581 ccgctcgccg tcgatccttt accaacgaag ctgtcgctaa ttactgggta gagcggggct
     2641 acaagttttg aattcgcaat gaggtgcagg cattcggagg aataactatg atgaaaatcg
     2701 atatcgacca gctcagcgaa gaggaactga ttgacctgaa tcatcggatc gtcgagcggc
     2761 tgcggttttt gacccagatg cgcgcgcatt cagagatgct ggattccaaa attggcgagc
     2821 gcgtgacgtt tcatccgaac ggccatccgc aactgttcgg gattctaacc cgctataacc
     2881 gaaaaactgt cacggtcatc acggatactg gacagcagtg gaatgttgcg ccgagtgtcc
     2941 tgcgccgggc tgacgcgcgc gcgacagcag gcggcggacc ctaagctagt ggggttaaaa
     3001 agaaactaat tcactgctcg tttctcctca ccattgctgc ctactcacta cctcatttcg
     3061 ggattgctaa gcggaaagcc gggaatcgct tgatgcagtc cggagttcct gccgtttgac
     3121 ttaaaagagc ggcggcgttt acagtcggcg gaatttttcg ggagaatttt ccacgatgcc
     3181 gcaaagctca ttcgaaccgg cttggtttgt gcgcaaggat ctcgacgggt tctttggatt
     3241 gatgatcgat aatctcatcc agctcattct gatcgtcagt ctttgccgcg agctgattca
     3301 cctgtcgaac gagtacattt ttggccggat ccttcccggt gcggccattt ctattctggt
     3361 ggggaatttt ttttacgcct ggcaggcgcg gcgtttagcg cgcgcgaccg gccgtgaaga
     3421 tgtgaccgcg ctcccttacg gcatcaacac cgtaagtctt tttgccttca tattttttat
     3481 catgctgccg acttatcttg aaacgaaaga tccggtttgg gcttggaaga tcggcctggt
     3541 ggcctgtttt cttaacggcg tgatcgaaat tatcggcgct ttcgtcgccg agacggtgcg
     3601 gcgtgtcacg cctcgcgcgg cgctattatc ggcgttagcg ggaatcgcga ttacatttat
     3661 ttccatggat tttaccttca agatttttgc tcggccgcta gttgccttgt tgcccatggc
     3721 aattattttt gtcgcttatt tttcgcgcca aaggttgcca ctcggcctgc ccggcggcat
     3781 gctggcaatc gccattggca ctgtcctcgg ttgggtgctg ggaaccatga acggcagtgc
     3841 tatcgcggcc tcttatgatt ttgcgctgcc gaagtattcg ggcgattcat tgtggcaggc
     3901 gatcacggat ccggcattcc tgagctactt ctcggttatt ttaccaatgg gaattttcaa
     3961 tgtggtcggc tcgctgcaga acatcgagag cgccgaagcg gccggcgatc ggttccgcac
     4021 gtttccatcg ctcatggcca acggcatcgg ttcggtcgtc gcggcgctat tcggcagcgt
     4081 ttttccaacc acgatctaca tcggtcatcc aggctggaag cgcttgggcg cgcgctccgg
     4141 ctattccgct ttgaacggta ttttcataag cgttctctgt ttgacgggaa cgattcagac
     4201 cgtgctcact gtcatcccgc tcgaagcggg aatcggcatt ctcctttaca ttggcatcat
     4261 cattgtcgct cagtccttcc aggaaacgcc caaggaacat gcaccggcgg ttgctctagg
     4321 catcgttcca gcattggctg gatggggctt gttgatggtg gagacgggcc ttcgggcagg
     4381 cggaaaatcc ctttatgcag tgggactgtc aaacttcgat caggagagtt tagcaatccg
     4441 cggtatgatt tccctggagc gtggctttat ttttacctcg atgattcttg ccgccatcgg
     4501 cgtagctttg atcgaacgca gatttgcaca agccggcgca tgggcggcag cggccgctct
     4561 gctctcggcg ttcggaatca tccatgctta tgatctcacc agcagcggcg ttacgagccg
     4621 cttcggttgg atggcggcac cggagttttg cgccgcttac ctgctattgg cggggttgtt
     4681 ttttcttatt gcgatgttgg cgagaaacgg cgctcggtaa gcgtcacttg ctggcggatg
     4741 tcggaccgtt ctctgcatac ggcagtggcg tgggattgta ctctcggctg ttgactgcgg
     4801 tcagattcca acgaccggca tcgtaggcga ggcgggtgac gccacaggga tcgatgtgaa
     4861 agctgcgata agcattcaga tgcgtgccgg tgatccggca gacgatcccg agaatcggaa
     4921 aattatggct tacgaccaaa atccgttccg cttcggaatg acgctgcacg atctggttca
     4981 gcccgttcca tgcacgctcg gcgacatcgg cgagccgttc gccgcccggg acgcggatct
     5041 cggcgggttc gctgcgccag cgcactaaga actccgcata attctctttg atttggttga
     5101 atgtcaaacc ctcgagcgcg ccgtgatcga gctcacagat cttttgttcg atgagtaccg
     5161 gcagtttgtg aggctgactg atatagtccg cggtctgcaa tgcgcggcga aggtgactcg
     5221 aatagatcgc gtgaatttcc tcggccctga gcagtgcagc gagatgctcg gcctggcgaa
     5281 cgccgacttc gctcaggtgc cgatcactgg agccctgaca acggccttga agattccaat
     5341 ctgtggcacc gtggcgcact aaaagaattt gcactcggag gctccgctgc ttagctctct
     5401 tcgagaaagg cgttgttgat tttcaccttg gctttggcct tcagattttc gagaaattta
     5461 actaaaactc tctggcgcgc ttcggcctga gcttgtttca tcaatgcggc tttgtctttg
     5521 tcgaactggg ccatatcgac tggctggctg tctttgaacg ccaaaacgta aacggcatct
     5581 ttctgagtat agagccgttc aggaatgggt ttttgcgccg aaaggctgat gggcccgcct
     5641 tttagctcgg cgagttcgcc gatcttgggt agctgctggg agctgcgcgg gaaccaaccg
     5701 gtttcttcaa ttttcaaatc accggtttga gccaacttgg atatctcttt ttcttttttc
     5761 aattgttcga gcaggctatt gcccttttgc gatgcaagct catacgcttt ggattctttc
     5821 aaattttttt cgatttgatc gcgcacgctc tcgaggggcg gagtcgcagg ttcttggcgt
     5881 gctttgagac ggatcagata atatccattc ttcccttcga tgacgggact gacatccttg
     5941 ggcgccagcg cgaaagcgct tttgtagaat tcctgatttt cgccaatttc gggaagagtt
     6001 tcacctgccg cgagccagtt gctgacggtg attttgacgc ccgattcttg cgccagcttg
     6061 ctcaactcag tgccggacag cgctttttcc cgatcgcgat cagccatttt cgctgcttcg
     6121 ctttttgcct tttcggcctt gagaattttt gtgatctccg gagtcgcttc cttgagcgat
     6181 tcag
//
LOCUS       DASULZ010000057         2958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_817900, whole genome shotgun sequence.
ACCESSION   DASULZ010000057 DASULZ010000000
VERSION     DASULZ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817900"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VFY96_01165"
     CDS             <1..826
                     /locus_tag="VFY96_01165"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5605092.1"
                     /translation="DVLHGGSGTLEPLKNENMLAPFMEYVVLAEVKDPKQWWGGHMWE
                     DNVKTNRFIYSFSADFSVPPFYNADLVKPEEITSFDDLLQPKWKGKIGMFEPRVPSAG
                     QGLWGYMMRAKGKEFLQRLAEQNLYIHRDGQQIAVGLAKGNLAIALGLAQRFVDPYIK
                     GGLPIKVLTSIKEGMGGSNGFGTVAVMGNAPHPNAAKVYINWLLGKEGQELYGRALTQ
                     GTRRLDVDTKWLARFNTPAAKDKITPEEFEKIRFYGEDVIINWREPAGDYARKILK"
     gene            1071..1787
                     /locus_tag="VFY96_01170"
     CDS             1071..1787
                     /locus_tag="VFY96_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006754093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX5605093.1"
                     /translation="MRYELYYWPSIQGRGEFVRLALEDAGADYADVARGPKGMAAMMR
                     LIESLSLRNPPFAPPFLKAGKLVIAQTANILLYLGPRLGLVPQSEAARLWAHQLQLTI
                     ADWVSEAHDTHHPIGGSLYYEDQRSEAKRRAADFTAERMPKFFDYFEGILQRNPKRSG
                     YLLGKQFSYVDLSLFQMIEGLRYAFPKTMKKLEKKYRRAVTLWDRVAARPRIASYLAS
                     PRRIPLNQEGIFRYYPELEQ"
     gene            1813..2475
                     /locus_tag="VFY96_01175"
     CDS             1813..2475
                     /locus_tag="VFY96_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX5605094.1"
                     /translation="MAIELYWGSGSPFAWRVMLTLELKRLPYESKLLEFSKQEHKTPA
                     YLQLNPRGKVPTLKDGDFVLYESIAIMAYLDRKYPTPPIFGTTAEQTGLIWRSVLECE
                     SYLLSAGDKVVRPLFFGKGLENVNEIQAAADVIRRELKMVNERLADSNWLVGGEISAA
                     DIAIFPLVQLLLRAAGKEAARPLDLGLIPLSKSFPNIARWVRRIEELPNYQRTYPPHW
                     KE"
     gene            2727..>2958
                     /locus_tag="VFY96_01180"
     CDS             2727..>2958
                     /locus_tag="VFY96_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605095.1"
                     /translation="MSSKNLHRLVFAVGLILTLSFGRSDAAEKTKITIGYSTTGPTAV
                     GLWTAKDIGAFDKYGVDPALIFISSSPVMVPAL"
BASE COUNT          703 a          771 c          840 g          644 t
ORIGIN      
        1 cgatgtgctt catggcggct cgggaacact ggaaccactg aagaacgaga acatgctggc
       61 gccattcatg gagtatgtgg ttctggcgga ggtcaaagat cccaagcaat ggtggggcgg
      121 tcacatgtgg gaagacaacg ttaagaccaa ccgcttcatt tattcattct cggcagattt
      181 ttcggtgccg cctttttaca acgccgacct ggtgaagcca gaggaaatca cgtcgttcga
      241 cgatctcttg cagccaaagt ggaagggcaa gatcggcatg ttcgaaccgc gtgtgccgag
      301 cgcgggccaa gggctgtggg gttatatgat gcgcgccaaa ggaaaagaat ttctgcaacg
      361 attggcggaa cagaatcttt atattcaccg cgatggccag cagatcgctg tcggcttggc
      421 caaaggaaat ctcgcgatcg cgttgggtct ggcgcagcga ttcgtcgacc catacatcaa
      481 gggcggcttg ccgatcaaag tgctgacctc gatcaaagaa gggatgggcg gcagcaatgg
      541 tttcggcacc gtcgccgtga tgggcaacgc gccgcatccg aacgcggcga aggtttacat
      601 caattggctg ctcggcaaag aagggcagga gctctatggc cgggcgctga cccaggggac
      661 ccggcgtctc gatgtcgaca ccaaatggct cgcgcgcttc aacacaccgg cggcgaagga
      721 caaaattaca ccagaagaat ttgaaaagat tcggttctac ggtgaggatg tgatcatcaa
      781 ctggcgcgag ccggccggcg attatgcgcg aaagatcttg aagtgagtga aagaaatttc
      841 cttgagcgaa ctcttcaccc gtttttggtt tatatcgcat tcgcggagta cgcagttaaa
      901 atccctccta acctcccttt gcgaaaggga ggaacgtagg accgaatagc acgacacgag
      961 taactcgcaa tcatctagaa cccatcgact aactctgggt gcactcttcg gacgatgatg
     1021 actcgttaga ttgtagagaa tcgagaaagt cagcgaaagg agtgtgacct atgcgctatg
     1081 agctttacta ctggccatcg attcaggggc gcggggagtt tgtccggctg gcgctcgaag
     1141 atgccggcgc agattacgcc gacgttgcgc gtgggccgaa aggaatggcg gcgatgatgc
     1201 gcctgataga aagtctgtcg ttaagaaatc cgccgtttgc gccacctttt ctcaaagccg
     1261 gcaaactcgt gatcgcacaa accgccaata ttctcttgta ccttggtcca cgtttgggac
     1321 tcgtgccgca aagcgaagcc gcgcgccttt gggcgcacca attgcaactg acgatcgccg
     1381 attgggttag cgaagcccat gatacgcacc atccgatcgg cggcagcctt tactacgaag
     1441 atcaaaggtc ggaagccaag cggcgcgcag cggatttcac cgccgagcgc atgcccaagt
     1501 tcttcgacta ttttgaagga atcctgcagc gcaacccgaa acgaagcgga taccttctcg
     1561 gcaagcaatt ttcctatgtc gatctctcct tatttcaaat gatcgaaggg ctgcgctatg
     1621 cttttccgaa aacgatgaaa aaactcgaaa aaaaatatcg gcgggccgtc acgctctggg
     1681 atcgggtcgc ggcccgcccg cgtatcgcca gttacctcgc ttcgccgcga cgcattcccc
     1741 tcaatcaaga agggattttc cgatactacc cggagctgga acaataaata atttttgcga
     1801 aggagagaat cgatggctat cgaactctac tggggcagcg gcagtccctt tgcttggcgg
     1861 gtgatgttga cactcgagct gaagcgactg ccgtacgaat cgaaacttct ggaattttca
     1921 aagcaagagc acaaaacgcc ggcatacctt cagctgaacc cgcgcggcaa agtgccgact
     1981 cttaaggatg gcgatttcgt gctctacgaa tcgattgcga tcatggccta tctcgaccga
     2041 aagtatccga cgccgccgat tttcggaacc acggcggaac aaacagggtt gatctggcgc
     2101 tcggtgttgg aatgcgaatc ctatcttctc agcgcgggtg acaaagtcgt gcgtccgctc
     2161 ttttttggca aaggactgga gaacgtaaat gagatccaag cggcggcaga cgtcatacgc
     2221 cgcgaattaa aaatggtcaa cgagcgtctg gcggattcga actggcttgt cggcggcgaa
     2281 atttctgccg cggatatcgc gatttttccc cttgttcagc tcctcttgcg ggctgccgga
     2341 aaggaggcgg cacggccgct cgatctcgga ttgataccct tgtcgaaaag ctttccgaat
     2401 atcgcacgct gggtccggcg tatcgaggag ttgccgaatt atcagcgcac gtatccgccg
     2461 cactggaaag agtagcgggg gcgcgatagg ggtcaagtag tctcttctac caagatttga
     2521 agtttctccc ccgtaagaaa ggagacttgt cattccgccg catgtcggaa tccagacttt
     2581 ttgtttgtct aacagaaaca atgaacctgg gtgccggtcc cggatcacgt ccggcgcaag
     2641 ctttcaccgg catggcggat agctaaacca ttgacagcgg cgcagctatc ttgttaggtg
     2701 gcggcggacg cgtcgccgct cgtgatatgt cttcaaaaaa tctccatagg ctggttttcg
     2761 ccgttgggct cattctgact ctgtcattcg ggcgatccga cgcggcagaa aaaaccaaaa
     2821 tcaccatcgg ctattcgacc accggaccaa cggcggtcgg gttgtggacg gcgaaggaca
     2881 ttggagcatt cgataaatac ggtgtcgatc ccgccttgat cttcatttcg tcgagcccgg
     2941 tgatggtgcc ggcattga
//
LOCUS       DASULZ010000058         2863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_513881, whole genome shotgun sequence.
ACCESSION   DASULZ010000058 DASULZ010000000
VERSION     DASULZ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513881"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..403
                     /locus_tag="VFY96_01185"
     CDS             197..403
                     /locus_tag="VFY96_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5605096.1"
                     /translation="MKISANVLEAVGNTPLVRLNHITAGITATICVKLEASNPGRSVK
                     DRIALAVVEDAEKKGVVEARRHDH"
     gene            375..1286
                     /locus_tag="VFY96_01190"
     CDS             375..1286
                     /locus_tag="VFY96_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5605097.1"
                     /translation="MLKPGGTIIEPTSGNMGVALAIVAAVKGYKCIFTIPDKMSIEKI
                     RRLRAFGAEVVVTPTAVPPESPQSYYSVARRLSEEIPGAYMPMQYDNIANREAHYAST
                     GPEIWKQTDGKLTHFVAGLGTAGTISGIAKYLKEKNPQCTVVGVDPEGSILAKAFKRE
                     KWSWSDSRPYKVEGIGEDFVPKNLLMQYIDDVVTVSDRDSFLTGRRLTREEGLFCGGS
                     SGAAVAGALEYARAQRLGKDSLVVVLLPDAGEIYLSKMYSDEWMRQNQFLGKSARVSD
                     ILESKGEEFPDCCRSKQPRRCAMQLPR"
     gene            1312..1572
                     /locus_tag="VFY96_01195"
     CDS             1312..1572
                     /locus_tag="VFY96_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5605098.1"
                     /translation="MFDKGNLVGSLCESSLFQRSMESPEVMELTVGALLEPPFPTVGA
                     DEDVHEVVQLLKSAPAVLVRDREKYHGILTRFDVVGHLGGQP"
     gene            1608..2723
                     /locus_tag="VFY96_01200"
                     /pseudo
     CDS             1608..2723
                     /locus_tag="VFY96_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015132131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
BASE COUNT          611 a          806 c          861 g          585 t
ORIGIN      
        1 cgcggcaagt tgacgttcac gtcggaatat cggacgagct tctaactgat agcgacaaac
       61 ttgtaattga tggccgccac tcggttgaaa aatccgctgt cggtgagcga aaggacagac
      121 ggcatagtat acttctgtta gctgggcaga attaatcagg ctgtcattga gctattcatg
      181 ctcgggggga atcgaaatga aaatctccgc taacgtgctt gaagccgtcg gtaacacacc
      241 gcttgtccgt ctgaaccata ttaccgccgg aattacagcc accatttgtg tgaaactcga
      301 ggcgtccaat ccgggaagga gcgtcaagga tagaatcgcg cttgcggtcg ttgaggatgc
      361 ggagaaaaag ggggttgttg aagcccggag gcacgatcat tgagcccact tcgggaaata
      421 tgggtgtggc gctggcgatt gtcgccgcgg tgaaggggta taagtgtatc tttacgattc
      481 cggacaaaat gagcatcgaa aaaattcgcc gcctgcgcgc ctttggtgcc gaagtggtcg
      541 tgactcccac ggctgtgccg cccgaatcgc cacaatcgta ctattcggtt gccagacgtc
      601 tttccgagga gatcccgggc gcctatatgc ccatgcagta cgataacatc gcgaaccgtg
      661 aagcccacta cgcgtccaca ggtcctgaga tctggaaaca gaccgacgga aagctcactc
      721 actttgtcgc ggggcttgga accgccggca caatctcggg aatcgccaaa tacctgaaag
      781 aaaaaaatcc gcagtgcacg gtggttggag tcgatcccga gggatcgatt ctcgccaagg
      841 cgttcaagcg ggagaaatgg tcatggagtg attcacgccc ctacaaggtc gaagggattg
      901 gcgaggactt cgtgccgaag aacttgctca tgcagtatat cgacgacgtc gtaactgttt
      961 ccgaccgcga ctcgtttctg acggggagac gcctaaccag agaggaagga ctgttctgcg
     1021 gcggatcgtc cggcgctgcg gttgccggtg ctctcgaata cgcgcgagca cagcgcctcg
     1081 gcaaggacag cctagtcgtg gtcttactcc cggacgccgg agaaatttat ttgtcgaaga
     1141 tgtattccga tgaatggatg agacaaaatc agttcctcgg caaaagcgct cgggtgagcg
     1201 acattctcga atcaaaaggc gaagagttcc cggactgctg tcggtcgaaa cagcctcgac
     1261 ggtgcgcgat gcaattacca cgatgaccga ttcggcgtct cgcaacttcc ggtgttcgat
     1321 aaaggaaacc tggtcgggtc gctgtgcgag agctcgctct tccaaaggtc gatggaaagc
     1381 cccgaggtga tggaattgac cgtcggggct ctgctcgagc cgccgtttcc caccgtcggg
     1441 gccgacgaag acgttcatga ggtcgtgcag cttcttaagt ctgcgccggc ggttctcgtc
     1501 cgggataggg aaaaatatca cggaatcctg acgcgtttcg acgtcgtcgg tcacctgggt
     1561 ggacaaccct gatggcgcga gcaaagcgag gcgggcatca gcagtcgatg aaatttgcca
     1621 cacgcgcgat ccatgtcgga caagagccgg aagccgcgac cggggcgatt actgttccga
     1681 tctaccagac gagcacgttc gcgcagagcg cgcccggtgt tcacaaaggc tacgactact
     1741 cgcgcaccga taacccgacg cgaaccgcgc tgcagacagc gctcgcaagc ctggagtccg
     1801 ccgagtactg tctcgccttt tcttccgcca tgcgcgcggc aacgactggg atgcttctgc
     1861 tcgaaagggg agatcacgtg gtctcctcgc gcgacgtgta cggcggcacc taccggcttt
     1921 ttcgcgcggt gttggaagtt tcggcctcac gtttcccttc gttgagcgcg ccatgactcg
     1981 gcggactcgg cttgtctgga tcgaatcgcc gaccaacccg cttctccgaa tcacggatat
     2041 caggttggca gcgaacgtcg ccccatcgcc gcggcgcctt gtgtctagtc gacaacacct
     2101 ttgcatcgcc ggttttccag cggccgctcg agcttggcgt cgatctcgtg cttcactcga
     2161 cgacaaaata tctcgcggga catgcggacg tcgtcggcgg cgcgatttgc ctcaatgaga
     2221 tcgcgctcta caagcggctc aagttcctgc aaaatgccgc cggcgcgacg ccgtctccgt
     2281 tcgactgctt tctctcgttg cgaggaataa agactctcgc gttgcgcatg cggtagcacg
     2341 aaaaaaacgc cacggagatc gcgcgtttcc tgcaagacca cgcgcgggtg cggcgggtct
     2401 actatcccgg cttgcataat catccggccc atgatgttgc cacttctcag atggacggct
     2461 tcagcggcat tgtctccttc gaggtgaagg gggggctcgc ggaagcgcgc cgcgttctgg
     2521 cgcgcctgcg cctgttcaag atcgccgaaa gtctcggcgg cgtcgagtcg ctcgtcgagt
     2581 tgccggctgt catgacccac gcctcgattc ccaaaccaga gcgcaaaaag gccggactcg
     2641 acgaagggct gatccgtttg tcagtgggga tcgagcacgt tcaggatctt atcgaagatc
     2701 tgaaacgggg actgggacgc tgacaggcaa gtctgacacc aatgcttcgt tgcgcgctcc
     2761 tcgtttaagt gcggaactca aaaattaggc gtggccgaca gacccggcgc ctgcggtctc
     2821 aggcctcggg ccgacggaga tcggacctgg ttgatacagc ggc
//
LOCUS       DASULZ010000059         3549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1021155, whole genome shotgun sequence.
ACCESSION   DASULZ010000059 DASULZ010000000
VERSION     DASULZ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021155"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..151
                     /locus_tag="VFY96_01205"
     CDS             <1..151
                     /locus_tag="VFY96_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HEX5605099.1"
                     /translation="QTISRATKISSVSKYLERIADHATNIAEMVIFMVKGKSIRHLKE
                     LPPSV"
     gene            155..871
                     /locus_tag="VFY96_01210"
     CDS             155..871
                     /locus_tag="VFY96_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5605100.1"
                     /translation="MKTGQKILVVEDEPDIRKLVNYNLAQEHFRVLEAEDGEQGLKMV
                     QREKPDLVILDLMLPGLSGIELCKILRERHETEKLPVLMLTAKAGEADRIVGLEMGAD
                     DYLAKPFSPRELVARVRAVLRRVESAKQADHPPVYDKGGLKIDFTTYEVFARGRSVRL
                     TLKEFELLRFLVQNPNRVLTRDQLLDRVWGGETFVTPRTVDVHIRRLRKAIEKDDSKP
                     QWILTLRGVGYKFDEKALEQ"
     gene            849..2681
                     /locus_tag="VFY96_01215"
     CDS             849..2681
                     /locus_tag="VFY96_01215"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5605101.1"
                     /translation="MKRLWNSSLIVKVFLSYFLVVALLFLSFYFSANLLLRHFYIRSL
                     STRMQQEAHLLARMVPFDEAGAALDSLCRQLSGELGARITIIATDGRVLCDSAEISEH
                     MENHGTRPEILEAMRTGTGTSIRYSTTVKYNMLYRAFHQTGANRDRFVRLAVPLTDID
                     NVIRAVRQSLLAGLAVASVMGLLLAWVFSRHLSRRFRRLVQFSGQVAAGSFPQNFFPD
                     TGSDEIALLEGHLNEMSLHLRANLSQVIEEKEKADSILRCMIEGVLVLDPKGRVLVMN
                     QQARAMFHVPDDLNVHGASVLEITRHPEVHRILEEIFTFDFSTQTYSKEVELDDERWF
                     RVNAVRLRDTRGNLQGSILVFHDVTDIKRFESMRSDFVANVSHELRTPLTAIRGYVET
                     LMHTPPADPKESRQFLAIIDRHSERLSRLTQDLLTLSDLESGKIQLSLQPLDASQLIQ
                     RVLEVFWDQAARKQVKLTDKIEPELPNILGDIDRLQQLFINLVDNALKYTPSGGEIIL
                     EARSLRPRNGTVAQVELAVTDSGVGIPEKDLPRLTERFYRVDKARSRDVGGTGLGLAI
                     VKHIVQAHKGDLTIESVLNHGTTIRVRLAAAIVARNQPHTVVCL"
     gene            2836..3465
                     /locus_tag="VFY96_01220"
     CDS             2836..3465
                     /locus_tag="VFY96_01220"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX5605102.1"
                     /translation="MPKFQFLPAETKFYEWFEKATGNLLEAAEALQDLLDNYVQVESK
                     VARITELEHRGDFIVHEVMHLLINTLIIPLDGEDIERLISALDDSLDKIEATAVRMLI
                     FRIEAPTEKARQLGQLIRSGASELHMAMPNLREKKNFRLVTEHIKEINTIENNGDRIL
                     REALVEIVAHKDDIFNLIRWKEIYEMLEETTDTMEDVADVLQRVVIKNG"
BASE COUNT          796 a          998 c          951 g          804 t
ORIGIN      
        1 gcaaaccatc agccgagcga ccaaaattag ctcggtttcg aaatacctcg aacgcatcgc
       61 cgaccacgcc accaatatcg cggaaatggt aattttcatg gtgaaaggta aaagcatccg
      121 ccatctcaaa gagttgcccc cttcggtttg atctatgaag accggacaaa aaattttagt
      181 ggtcgaagat gagccggata ttcgcaagct ggtcaactac aacctagcgc aagagcactt
      241 tcgggtgctg gaagccgaag acggcgagca aggtctgaag atggtccagc gcgaaaagcc
      301 cgatctggtc attctcgatt taatgctacc cggcctctcc ggcatagagc tctgcaagat
      361 tctgcgcgag cggcatgaga cggaaaagct tccggtgctg atgttgacgg ccaaggccgg
      421 cgaagccgat cgcatcgtcg gtctcgagat gggcgctgac gactacctcg ccaagccctt
      481 cagcccgcgc gagctcgtcg cgcgcgtgcg cgcggtgctc cgccgggtcg agagcgccaa
      541 gcaagcggac catccgcccg tctatgataa gggcgggctc aagatcgact tcacgaccta
      601 tgaagtcttc gcgcgcgggc gtagtgtcag actgactcta aaggaatttg agcttttgcg
      661 cttcttggtc caaaatccca accgtgtgct cacccgagat caactgttgg acagggtttg
      721 gggcggtgaa accttcgtga cgccgcgcac cgtggatgta cacattcgcc ggttgcgcaa
      781 agcgattgaa aaggacgaca gcaagcccca atggatcctg acgttgcgcg gagtcggcta
      841 caaattcgat gaaaaggctc tggaacagta gtcttattgt aaaagtattt ctctcgtatt
      901 ttctcgtcgt cgcgcttctc ttcctgagct tctatttttc tgccaacctg ctgctgcggc
      961 atttctacat ccgttctctg agcacgcgca tgcaacaaga agcgcacctg ctcgcgcgca
     1021 tggtgccctt cgatgaagcg ggtgcggccc tggacagcct ttgccgccag ctctcgggcg
     1081 agctcggcgc ccgcatcacg attattgcca ccgatggccg tgtactctgt gactccgctg
     1141 agatctctga gcacatggaa aaccacggta cccgcccgga aattctcgag gcgatgcgga
     1201 ccggcacggg aacttcaatt cgctatagca cgacggtcaa atacaacatg ctctaccgcg
     1261 ctttccatca gaccggagcc aatcgagatc gctttgtccg gctggctgtg ccgttgaccg
     1321 acatcgacaa tgtcattcgg gcggtccgtc agtctttgct cgccggcctt gcggtggcct
     1381 ctgtcatggg tcttctgctg gcgtgggtgt tttcgcgcca cctgagccgg cgttttcgaa
     1441 gattggtgca gttttccggg caggtcgccg cgggctcatt cccgcaaaat ttttttcctg
     1501 acaccggcag cgacgagatt gccctgctcg aaggtcatct gaacgaaatg agtcttcatc
     1561 tgcgcgccaa cttgtcgcaa gtcatcgagg aaaaggaaaa agcggattcg atcctgcgct
     1621 gcatgattga aggcgtcctg gtgcttgatc ccaaaggccg tgttctggtg atgaaccagc
     1681 aagcgcgcgc tatgttccat gttcccgatg acctgaatgt tcatggcgca tccgttctgg
     1741 aaatcactcg ccacccggag gtccaccgaa ttctcgaaga gatatttacc ttcgatttct
     1801 cgactcaaac ctattcaaaa gaagtggagc tcgatgacga gcgctggttt cgcgtcaacg
     1861 cggtacgttt gcgggatact cgggggaatc tccaggggtc gattctggtt ttccatgatg
     1921 tcacggatat caaacgcttc gaatccatgc gttcggactt cgtcgccaat gtctctcatg
     1981 agctgcgcac gccgctcacc gcgattcgcg gctatgtcga gactctcatg cacacgccgc
     2041 cggcggaccc caaggaaagc cggcaatttc tcgccattat cgatcgccat tccgaaaggc
     2101 tgagccgttt gacgcaagat ctgctgacgc tttccgactt ggaatcgggc aagatccaat
     2161 tatcgctgca gccgctcgat gcgagccagc tcattcagcg tgtcctcgaa gtcttctggg
     2221 atcaagcggc ccgcaagcaa gtcaagctca ccgacaaaat cgaaccagag ctgccgaaca
     2281 ttctcggcga tatcgatcgt ttacagcagc tgttcatcaa ccttgtcgat aatgcgctca
     2341 aatacacgcc gagcggcggt gaaattatcc tcgaagcgcg ttcgcttcgg ccgcggaacg
     2401 gcactgtcgc ccaagtcgag ctcgctgtta ccgactccgg cgtgggaatt ccggaaaaag
     2461 acctgccccg tctcaccgaa cgattttacc gagtcgacaa ggcgcgctcg cgagacgtcg
     2521 gcggcaccgg tttggggttg gcgattgtca aacacatcgt gcaggctcac aaaggtgatc
     2581 tcaccatcga aagcgttttg aatcacggca cgacgatacg ggtccgcttg gccgccgcaa
     2641 ttgtcgcacg aaatcagccc cacacggtcg tctgtctctg aacatcctct ctctctctct
     2701 ctctctctct ctgcagctgt cccgttgttt tggttgaagc cgttttggac gcatgctaag
     2761 ttgcatcgcg tccatcagct ttgaatccaa ataatcaaaa caatttccat aacttgagca
     2821 gcttttgggg tgaccatgcc gaaatttcag tttctgccgg ccgagaccaa attctatgaa
     2881 tggtttgaaa aagccaccgg taatttgctc gaggcggcag aagccctgca ggatcttctt
     2941 gataattatg tgcaggtcga aagcaaagtc gcgcgcatca cggaattgga gcatcgcggc
     3001 gacttcatcg tccatgaggt catgcatctg ctcatcaata ctctgatcat tccgctcgac
     3061 ggcgaagata tcgaacggct gatctcagcc ctggatgact cattggataa gatcgaagcg
     3121 accgcggtgc gcatgttgat ttttcgcatc gaggcgccaa cggaaaaagc gcgccaactc
     3181 gggcagctca tccgcagcgg cgcgagcgag ttgcacatgg cgatgccgaa tctgcgggag
     3241 aagaaaaact tccggttggt gaccgagcac atcaaggaaa tcaataccat cgagaacaac
     3301 ggcgatcgaa ttctgcggga agcgctagtt gagatcgtcg cccacaaaga tgacatcttc
     3361 aacttgatcc gctggaaaga gatttatgag atgctcgaag agactactga taccatggaa
     3421 gatgtggccg atgtactaca gcgggtcgtg atcaaaaatg gctgatccgc tcctggttct
     3481 gacggtcctg atcatcgccg ttgcgcttgt tttcgactat gtgaacggct ttcatgacgc
     3541 cgcaaattc
//
LOCUS       DASULZ010000060         4142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_615367, whole genome shotgun sequence.
ACCESSION   DASULZ010000060 DASULZ010000000
VERSION     DASULZ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615367"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..498)
                     /gene="rnpB"
                     /locus_tag="VFY96_01225"
     ncRNA           complement(135..498)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFY96_01225"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(754..1467)
                     /locus_tag="VFY96_01230"
     CDS             complement(754..1467)
                     /locus_tag="VFY96_01230"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEX5605103.1"
                     /translation="MQAQIEILASLQTVDREIKEQTSRKQKLLDELRVMEKQILVKKG
                     EIDGLKAVHAEKEKFRAEKDRVLQDEGRKAMDKRMRMNRIKNVKELQALQREIELMRQ
                     TNGELEEELIKIMQEMDEIKTQIQTKESEMAAMQQEWQNKQNELQSEITGIDAAVSEA
                     ASRRQSIAAQVAGDLISRYELIFSRRGGTAVVEVAGGICQGCYMNLPPQLWNEIIRNE
                     KLHLCPSCQRILFVKPRAE"
     gene            complement(1695..3077)
                     /locus_tag="VFY96_01235"
     CDS             complement(1695..3077)
                     /locus_tag="VFY96_01235"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5605104.1"
                     /translation="MDRIAGELARLIGTERISVDPSQLDELSWDALSEGRIHPQHRPE
                     LHRPTCIVRPQSTDEVRRVVEFANTEMISVVPFGGGSGLMGGALSIRPCIALDLRHMN
                     RIIDVDTEAHTARVEAGVVLESLDTTLSQSGFILGHDPWTVPVATIGGAISTDSVGYR
                     AGIYGSMGEQVLGLEAVLPNGEVLRTPPVSKRSVGINLNGLLIGGEGCFGVVTEATIR
                     IFPKPETRVFCGFLFSSFEEGFAAIQKLFHKRVRPALLDFGDDEDEHEAGALLYLVFE
                     GNERVVAAESQLAIGICSDHSGKSLNPEQPEQFWRERHDIARRFMENRRQRRERGRDG
                     IFRDWIHVALPASKVVPFRRAAQDVIRRRGVLLQESGLWVQPELFSMRLAAEDDALPN
                     AQLALEETVEALLRLAQQMGGSMEYTHGVGIKLAPLMANEHGYGLEVMRQIKRAVDPR
                     NIMNPGKMGL"
     gene            complement(3178..>4142)
                     /gene="mgtE"
                     /locus_tag="VFY96_01240"
     CDS             complement(3178..>4142)
                     /gene="mgtE"
                     /locus_tag="VFY96_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX5605105.1"
                     /translation="SRLRADMTVDEAISYLRKDARMREKTVYYVFVVDAQEHLLGVVS
                     FRDLLVSPGDKLVRDVMRNEVISAPEDLDQEALSKLFMRHHLLMIPIVDAEGRIKGVV
                     NVDDIVDVVQEEATEDIQKIGGVETLEGPYLQVPLLVMIRKRAGWLAALFLGEMLTAT
                     AMGQFEAEIARAVVLALFVPLIISSGGNSGSQATTLVIRAMALGEVRLRDWWRVIRRE
                     VVTGLGLGAILASIGLVRILLWQFLFDAYGEHYFLVALTVALSLVGVVLWGSLAGSIL
                     PFILRGLGFDPASASAPFVATLVDVTGLVIYFTIAAGVLSGTLL"
BASE COUNT          958 a         1221 c         1038 g          925 t
ORIGIN      
        1 gaaataaaat cgagcgccaa aatccgccgt tcagaactca cggacagcag aagagcaggc
       61 tcacaaaatg tgcgggatct aaagaaagac ggatgtctaa gcgttatgag acgtcggagg
      121 aagacaggct cgcgggctaa agtgaatcga taagccggat tctgtggtgg ctcataaaaa
      181 gaaccacagc gatcattcat cttgcccctc gattgctcga ggggtcgagc gaccataccc
      241 gagggctatg agcggaccac tcaaccctcc tatttggcct tgctccaggt ggggtttgcc
      301 atgcgttcgc cattactggc gaaccggtga gctcttacct cgccttttca accttaccag
      361 ccacacaggc tggcggtgtt ttttctgtgg tactttcccc cgatcactcg gagccgccgt
      421 tagcggccac cgtgtcctat ggagtccgga ctttcctccc cttgcgcaag cgcaaagagc
      481 gatcgcttaa tccactttag cccgagcgat ttgatcagcg ctgggtttag gcatgagtgt
      541 tacgtcgacg attggctagc tcacttgtag aggatacgtt aggactaagg ctgacgcaaa
      601 tcgcgaggcg agcgcactgc cgaatcgcac tcggcaaaaa cgcgtgagct gggtctagtc
      661 ttcggaaggt aattagcccg aaagcttgcg cgaaaaatcg ctcaagttat ggaatcaaag
      721 agaaacaaac gatccgctga tgtcttaacg ccgctattca gcgcgaggct tgacgaaaag
      781 aatccgttgg cagcttggac ataaatgaag cttttcgttt cgaatgatct cattccatag
      841 ttggggcgga agattcatgt aacagccttg acagattcct cccgctactt cgaccactgc
      901 cgttcccccg cgacgcgaga aaatcagctc atagcgggag atcagatccc ctgccacctg
      961 cgctgcgatg ctttgccggc ggcttgctgc ctcggaaacc gcggcgtcaa tcccagtgat
     1021 ctccgattgc agctcgtttt gcttgttttg ccattcttgc tgcatcgctg ccatttcgga
     1081 ttcctttgtt tgaatctgtg ttttaatctc gtccatttcc tgcatgatct tgatgagctc
     1141 ctcttcgagc tcaccgttgg tttgtctcat gagctcgatc tcgcgctgaa gagcttgcag
     1201 ttccttgacg tttttaatgc ggttcatgcg catccgtttg tccattgcct ttcgaccttc
     1261 gtcctgcaac acgcggtctt tttcggcacg aaacttttcc ttttcggcat gcaccgcttt
     1321 taatccgtcg atttcccctt tcttgaccag gatttgcttt tccattaccc gcaactcgtc
     1381 gagcaatttt tgcttccggc tggtttgctc tttgatttcc cgatcaacag tttgtaacga
     1441 tgccaaaatt tcgatctgtg cttgcaagtc ttaaccctcc aagtaaaaaa aaatgcaccc
     1501 tttggggtgc atttctattg atggacgcta gcggaaagat atcgattgag tcttaatctg
     1561 ttcactccag actacctcta tttatctata tcgctttcca gccgttgtca aagccggttt
     1621 tcacgctcgt gctattcgac ggcgacaatc gcttctttga cgacgagcgg tttcttagct
     1681 tcacgactct tgttttaaag acccatcttg cctgggttca tgatattcct cggatcgacg
     1741 gcccgcttga tctgacgcat gacttccagc ccatagccgt gctcattcgc catcagcggc
     1801 gcgagcttga tcccgacgcc atgggtatac tccatcgagc cgcccatctg ctgcgcaagc
     1861 cgaagcaatg cttccaccgt ctcttcaagt gccaactgtg cgtttggcaa agcgtcatct
     1921 tcggcagcta gcctcatcga aaagagctcg ggttgcaccc acagcccact ctcctggagg
     1981 aggactccac gccgccggat tacgtcctgc gctgcccgtc taaatgggac taccttcgag
     2041 gctggcagcg ccacatgaat ccagtcacga aaaattccgt cgcggcctcg ctcgcgccgc
     2101 tgccggcgat tttccataaa gcggcgcgca atatcgtgcc tttcgcgcca gaactgttca
     2161 ggttgctctg ggttaagcga tttaccgcta tggtcagagc agatcccgat tgcgagctgc
     2221 gattccgctg cgacgactcg ctcattacct tcgaaaacta gatacaagag cgctccagct
     2281 tcgtgctcat cttcatcgtc gccgaaatcg agcagcgccg gccgtactcg tttgtgaaac
     2341 aatttctgga tcgcggcaaa accttcctcg aacgaagaaa aaagaaaccc gcagaacacc
     2401 cgcgtctcgg gttttggaaa aatgcgaatc gttgcctctg taacaacacc gaagcagccc
     2461 tcgccgccga tcagaagacc gtttagatta attcctaccg atcgcttcga tacgggcggc
     2521 gtgcgcagaa cctcgccgtt cggcaacacc gcttccaaac cgagcacctg ctcgcccata
     2581 gaaccataga tacccgcgcg atagccgaca ctatcggtag aaatcgcgcc accgatcgtg
     2641 gcgacaggca cagtccaagg atcatggccc agaatgaaac ccgattggct caacgtggta
     2701 tctagcgatt caagaacaac accggcttcg acccgcgccg tatgcgcctc cgtatcgaca
     2761 tcgatgatgc ggttcatgtg acgcagatcg agtgctatgc aggggcgaat cgacaacgca
     2821 cctcccatca acccggaccc gccgccgaac ggcaccacgg agatcatttc cgtattggca
     2881 aattcaacga cccggcgaac ctcgtcggtg gattgcggtc ttacaatgca agtggggcga
     2941 tgcagctcgg gtcggtgctg tgggtgaatt cgcccttcgc tgagagcgtc ccaggaaagc
     3001 tcgtccagtt gagaaggatc tacggaaatt cgctcggttc ctatgagcct cgccagttcg
     3061 ccggcaattc gatccataaa gagttacacg cgaaagaatc ttatctcaat caaatagacc
     3121 gggaaagcgg acagatctac cgtgacaaag gcaaaccgga ttgcttgtag agaaccgcta
     3181 gagcaatgtg ccgctcagca ctccggcggc gatggtgaag taaatcacta gacccgtcac
     3241 gtcgaccaaa gtcgccacga acggcgccga ggcgctcgcg gggtcgaacc caagaccgcg
     3301 caaaatgaaa ggcaggatgg agcccgccaa tgaaccccac agcacgacgc cgaccaggct
     3361 caaggcaacg gtcagcgcga ccagaaaata atgctcgccg taagcatcga ataaaaactg
     3421 ccaaaggagg atgcgaacga ggccaatgga agcgaggatc gcccccaatc ccaagccggt
     3481 cacgacctca cggcggatga cacgccacca atcgcgcagc cgaacttccc ccagcgccat
     3541 tgcgcgaatg acgagggtgg tggcttgcga gcccgaattg ccgccgctgc tgataatcag
     3601 cggcacgaac aacgccaaca ccacagcgcg ggcaatttcc gcctcgaatt gccccatcgc
     3661 cgtggcggtg agcatttcgc ccagaaacag cgcagccaac cagccggcgc gtttacgaat
     3721 catgaccaac agcggcacct ggagatacgg cccttcgagc gtctccacac cgccgatttt
     3781 ttgaatatct tcggtcgcct cctcttgcac gacatcgacg atatcatcga cgttgacgac
     3841 gcccttgatt cgcccctccg catcgacaat cggtatcatc aagagatggt ggcgcataaa
     3901 aagcttgctc aaggcctctt gatctaaatc ttcgggagcg gaaataacct cgttgcgcat
     3961 gacatcgcgc accaacttgt cgcccgggga taccagcaga tctcgaaacg agacaacgcc
     4021 gagcaggtgc tcttgcgcgt cgaccacgaa cacgtagtaa accgtctttt cccgcatgcg
     4081 cgcgtcttta cggagatagc tgatcgcttc atcgactgtc atatccgcgc ggagacgaga
     4141 at
//
LOCUS       DASULZ010000061         2517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1427122, whole genome shotgun sequence.
ACCESSION   DASULZ010000061 DASULZ010000000
VERSION     DASULZ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427122"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VFY96_01245"
     CDS             complement(<1..377)
                     /locus_tag="VFY96_01245"
                     /inference="COORDINATES: protein motif:HMM:NF013243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX5605106.1"
                     /translation="MNNVLSYMTFIPVAGAAIVLALPNNAKLIRWVSAIATVPPLLMA
                     IWLFKNFDRAVPGFQFVEDYTWIPAYNISYTMGVDGLSISMILLTALLSFLCIFASFG
                     IDKAVKGYFALFLLLDAGMMGVF"
     gene            complement(399..2441)
                     /gene="nuoL"
                     /locus_tag="VFY96_01250"
     CDS             complement(399..2441)
                     /gene="nuoL"
                     /locus_tag="VFY96_01250"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015471303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX5605107.1"
                     /translation="MESPIQTDFIRWIVFLPLLGAIVNGLLGAKIQKSMGKSAIGVLA
                     CTPVVIAFGLAVYSFFILKGLEPEKRFLIDNLYPWIYLGSLKVDMAFMIDPLSTVMIL
                     VVTGIGGLIHIYATGYMHEDKAFWRFFAYLNLFTAAMLTLVLGDSLLVLFVGWEGVGL
                     CSYALIGFWYTDHANARAGNKAFIVNRVGDFGFVLGMFLLFWSLDAQGHGSLTFREMI
                     KWAPSIKDSMLWGIPVVTLATLFLFVGATGKSAQIPLFVWLPDAMAGPTPVSALIHAA
                     TMVTAGVYMTARMNVFFSMAPVTLEVIAAIGVATALLAATIALTQFDIKKVLAYSTVS
                     QLGYMFIGVGVGGYAAAVFHLMTHAFFKACLFLGSGSVIHAMHHEQDMRKMGGLKNWM
                     PITFVTFFISVLAIAGFPPFAGFFSKDEILWLTFTSPHGNKLVWFLALCGAGLTAFYM
                     FRQLFMTFYGDCRADHHTQEHLHESPHIMTMPLVILAIGAVVAGFIGLPGVLGGSQFA
                     QWLEPVIEAHEEAHASHVLEWGLMAVSVSIAAVGVLMAYLMYRRESLSSAIFVNLAGG
                     FFYRLFDRKWYFDEVYQVIFVSGTLRLARLGSLFDQYVIDGIVNGLATLTRSIAWLEG
                     LFDQYVVDGIVNLIGNLTFAIGNKFRRVQTGNINSYLYGILIAVVLLIIAKLRYAS"
BASE COUNT          699 a          694 c          655 g          469 t
ORIGIN      
        1 cagaaaacgc ccatcatgcc ggcatcgagc aggagaaaca gcgcgaaata acctttgacc
       61 gccttgtcga tgccgaagga ggcaaagatg cataaaaagc tcaagagcgc cgtcaaaagt
      121 atcatggaaa tactcaagcc atcgacgccc atggtataac tgatgttgta agcagggatc
      181 caagtataat cttcgacgaa ctgaaatccc ggaaccgcgc gatcgaaatt cttgaagagc
      241 cagatcgcca tgagcagcgg cggcaccgtc gcgattgccg aaacccaacg aatcagcttg
      301 gcgttgttcg gcaacgcgag cacaatggcc gctccagcca ctggtatgaa agtcatgtaa
      361 ctgagcacgt tgttcatctt ttccgttctc cctggagatc agctcgcgta acgcagcttg
      421 gcaataatca gcaacaccac ggcgatcaaa attccgtaaa gatagctgtt gatgttgccg
      481 gtttgaactc ggcgaaactt gttgccgatc gcgaaggtca gattgccgat caaattgacg
      541 ataccgtcca caacgtattg atcgaacaaa ccctcgagcc aagcaataga ccgggtcaat
      601 gtggcgaggc cgttgacgat accgtcgatc acatactgat cgaacagcga acccagccgc
      661 gccagccgca aagtaccgct gacaaaaatc acctgataaa cttcgtcgaa ataccacttg
      721 cgatcgaaca gacggtaaaa gaaaccgccg gcgaggttca caaaaatcgc ggacgacagc
      781 gattcgcggc gatacattaa ataggccatc aggacaccaa ccgcggcaat ggataccgac
      841 acggccatca aaccccattc cagcacgtgc gaagcatgtg cctcttcatg cgcttcaatc
      901 accggctcga gccactgcgc aaattgactg ccgccgagca caccgggcag tccgataaat
      961 ccagccacga ccgcgccgat cgccaagatc acgagtggca ttgtcataat gtgcggcgat
     1021 tcatgcagat gctcctgagt gtgatgatcc gcgcggcagt cgccataaaa cgtcatgaag
     1081 agctggcgaa acatatagaa ggccgttagc ccggcgccgc agagcgcgag aaaccagacg
     1141 agcttattcc cgtgcggact tgtgaacgtg agccagagga tctcatcttt ggaaaaaaat
     1201 ccggcgaagg gcgggaatcc cgcgatcgcg agcaccgaga tgaagaaagt cacgaaagtg
     1261 atcggcatcc agtttttgag gccgcccatc ttgcgcatat cttgctcatg atgcatcgcg
     1321 tgaatcacgc tgccggagcc gaggaagaga caagctttga aaaaagcatg ggtcatcagg
     1381 tgaaaaaccg ccgcggcata tccgcccacg ccaacgccga tgaacatata tccgagctgg
     1441 ctaacggtcg aataggcaag gactttcttg atgtcgaatt gagtgagcgc gatcgttgct
     1501 gcaagcagcg ccgtggcgac accgatcgcc gcgatcactt ctaaggtgac cggcgccatg
     1561 gaaaagaata cgttcatgcg agccgtcatg tagacacccg cagtgaccat cgtcgcggcg
     1621 tggattaacg cgcttaccgg cgtcggaccc gccatggcat ccggcaacca gacaaacagc
     1681 gggatctgcg ccgactttcc ggtggcgccg acaaaaagaa acaaagtggc gagagtgacc
     1741 acgggaatgc cccagagcat cgaatccttg atactgggcg cccacttgat catctcgcga
     1801 aatgtgaggc tgccgtgacc ctgagcatcg agggaccaaa acagcaaaaa catgccgagg
     1861 acaaagccga aatcaccgac ccggttcacg atgaatgctt tgttgcctgc gcgcgcattc
     1921 gcatgatcgg tgtaccagaa tccgatcaac gcataggagc acaatcccac gccttcccag
     1981 ccgacaaaca aaaccagcaa gctgtcgccc aaaacgagcg tgagcatcgc cgcggtgaac
     2041 aaattcaagt aggcaaagaa gcgccaaaac gctttgtcct cgtgcatgta gcctgtcgca
     2101 tagatgtgaa tcagcccgcc gatgcccgtg acgacaagaa tcatcacggt ggaaagcggg
     2161 tcgatcatga aagccatgtc gaccttgagc gagccaagat aaatccacgg atagagattg
     2221 tcgataagga agcgtttttc cggctcaaga cctttgagga tgaagaaact gtagaccgcg
     2281 agtccaaacg cgatcacgac cggcgtacag gcaagtacgc cgatggcgct cttgcccatg
     2341 cttttttgga ttttcgcgcc cagcaatcca ttaacgatcg cgccgagcaa aggcaggaac
     2401 acgatccagc gaataaaatc tgtttgtatc ggactctcca cgtagcctct attttcgatt
     2461 ttccattttg gattttcgat tacggaaacg gatgacgctt ggaatgtttt tcatcga
//
LOCUS       DASULZ010000062         5032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1427258, whole genome shotgun sequence.
ACCESSION   DASULZ010000062 DASULZ010000000
VERSION     DASULZ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427258"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..579)
                     /locus_tag="VFY96_01255"
     CDS             complement(181..579)
                     /locus_tag="VFY96_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004927853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5605108.1"
                     /translation="MSLAKAHLRVARPTDQLAAVVKFYREGLGLEVLYEFQDHDGFDG
                     VMLGHKSAGYHLEFTRKRGHAAGKAPSQDNLLVFYIPDRTEWEEAAQRIEKLGYPPVP
                     AFNPYWDIQGKTFQDPDGYRVVLQNASWTA"
     gene            811..1857
                     /locus_tag="VFY96_01260"
     CDS             811..1857
                     /locus_tag="VFY96_01260"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605109.1"
                     /translation="MTITRLRSSLLRLKQRCHSTALALVAVCLWAQAETALASEAKPL
                     AKVVIGYGSADGGVAVLGFAKDTKLFEKHGLDVVLVGMGTGSVSLRALIAKDLQISSL
                     SGSGLVQAALQGADTVLIAALINGFVFKIFSVPEISTPVQLKGKKLGVSRYGATSDFA
                     VRLALKKWGLNPERDVNILQIGTSQDTVRALQAKMLDAGVTSGTSSLVARKSGFRELG
                     DLADLGLDYPTSAIGTTRSYLQKNEANVKRFMLAYIEAIHDFKRNKEAAIAVLKKYSR
                     NEDREVLEHNYAEIASRYLPMPIPTVEGIRTILSELASTTPAAKNADPEQFVSYKIAR
                     EIEASGFVKRLYEK"
     gene            1910..2674
                     /locus_tag="VFY96_01265"
     CDS             1910..2674
                     /locus_tag="VFY96_01265"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605110.1"
                     /translation="MTTDYTKKLLPRFRYGVIQPRAHEEVQRARSYQLYRLLPLEFME
                     ISTGLGLENYTKEGVEKAINNYWACVDRLAKEKVDHIIFSGAPISAMLSRPRVLQLLR
                     ETKERTGISADAPLEALIAAMKHLGLTKLAVGSRWADPVNDAIKRYLEAGDIEVVGIT
                     KRNQWASDSFAMTLEQGLEMALDVGREAARTWPEAEAISVPGGAAMSLHVIPAIEEEY
                     GKPAFTNMSAEVWNDLVRPGIIAPVQGWGRLLAGMK"
     gene            complement(2797..3732)
                     /locus_tag="VFY96_01270"
     CDS             complement(2797..3732)
                     /locus_tag="VFY96_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5605111.1"
                     /translation="MKPRNQLTLLKRRVLPPPRQMTGKERRRINPNRGVIKVAERYTD
                     ILFSRFIYPFLGNFWNPYCWVLEQRFSLAETTVAPLGWPNDLAPLRVLLITDIHGGIF
                     LKPQTLSGILRALMQMKPDLVVIGGDLVSGHVNEAIPYFDGLGQLSNAPLGAWFCLGN
                     HDYFGGDPVELTENLAGIGIQTLKNQSVSLLHHGKALVLGGIDDRIMGKPDWQSLTAQ
                     QRPHLLMAHNPDFFYEAEQRGIPLMVSGHTHGGQIRFPRGPAIIRQSRYCLDEGVYAY
                     GSTLLVVSRGLGSVMLPFRWGADPEAILIEVVPPR"
     gene            4028..>5032
                     /locus_tag="VFY96_01275"
     CDS             4028..>5032
                     /locus_tag="VFY96_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605112.1"
                     /translation="MLIHSKCFIALRLPIALATVLLLLVFVVSEAAESPRPGGELVFA
                     VGEIPPSFDGHRETTFGMLHPVAPHYSTLLRFDPQNYPKIVGDVAEKWSSSKDGLTVT
                     VMIRKGIKFHDGTPLTAQDVKATYDKIIFPPEGVASARKALYSMVDKVEAPDDATVVF
                     KLKHISASFLANLASPWNFIYSAERLKKDPRWYEKNVMGSGPFIFAEHVAGSHWAGKK
                     NPNYFLPGRPYLDGFRAVFIRDTAPRIAAVRSGQAIVEFRGFNPAARDDIVKSLGNKV
                     VVQESPWACNILVTLNNEKKPFNDARVRRALTLAIDRNEASKALSQISVMKYIGGVFR
                     P"
BASE COUNT         1103 a         1391 c         1464 g         1074 t
ORIGIN      
        1 taaccgccga ttcgatcaac cgggcactta cgattataac tgctcggtcc atccttatat
       61 gatgggccgc gtgatcgtgc gtgctcgatg aagatgccgg cgaggcatgc ggctgagccg
      121 acactgcgcg acgagccgga aggacgcgcg gcgccggctc atgccatgtg cggcagtagc
      181 tcaagcagtc caactcgcgt tctgcaaaac tactcggtag ccgtcgggat cttggaacgt
      241 ttttccctgg atgtcccagt aagggttgaa cgccgggacg ggtgggtagc cgagcttctc
      301 gatccgctgc gcggcttcct cccactccgt tcgatcggga atgtaaaaaa ccagaagatt
      361 gtcctggctg ggtgctttcc cggctgcatg cccacgcttg cgcgtgaact cgaggtggta
      421 tcccgctgac ttatgaccca gcatgacacc atcgaagccg tcgtgatcct ggaattcgta
      481 aagaacttcg aggccgaggc cctcgcgata aaatttcacg accgcggcga gttgatcggt
      541 gggtcgcgcc acgcgcaaat gagctttcgc gagactcatc gcaaggtcat ccgattttcc
      601 catctgctct gtcaagtcct gcttctgtcc cgagcaagac ctacgtcgtc tcagaaggat
      661 ttccgcgtcg aggccaggcg ccgaattgct tgcctgtttc gctgtgatcc gaaccggcta
      721 ccgattcgat tgacagcgag cagatcagcg cataagtttt tagcgagccg tgccggtccg
      781 ttcgcgaacg gctcgcggcg ggaaaatggg atgactataa ctcgcctccg atcttctctc
      841 cttcgcctaa agcagcgctg ccattcaact gccctcgcgc tcgtggccgt ttgtctctgg
      901 gctcaagcgg agactgcgct ggcaagcgaa gccaagccgc tcgcgaaagt cgtcatcggc
      961 tacggttctg cggacggcgg cgtcgcagtc ctagggtttg ccaaggacac gaaactattc
     1021 gagaagcatg gtctcgatgt ggtgctggtt gggatgggca ccggatcagt ctcattgcgg
     1081 gcgctgattg ccaaagatct gcagatttct tcgctctcgg ggtcgggttt ggtgcaagcc
     1141 gcgctgcagg gcgccgatac ggtactcatt gcggctctca tcaatggatt tgttttcaag
     1201 attttcagcg tgccggagat ttccacaccg gtgcagctca agggcaagaa gttgggcgtt
     1261 agccgttatg gcgcgacctc cgatttcgcg gttcgtctgg cgctcaagaa atggggtctc
     1321 aatcccgagc gcgacgtcaa tattcttcaa atcggcacat cgcaggacac ggtccgcgcg
     1381 ttgcaggcca agatgctcga tgcgggggtc accagtggca cctcgtcgct ggtcgcgcgc
     1441 aagtcgggtt ttcgcgagtt gggggatctc gccgacctcg gcctcgatta tccgacctcg
     1501 gccatcggca cgacccgctc ttatttgcaa aaaaacgaag cgaacgtgaa acgatttatg
     1561 ctcgcctaca tcgaagcgat ccatgatttc aagcgcaata aagaagccgc gatcgcggtg
     1621 ctgaagaagt attcgcgcaa cgaagaccgc gaggtgctgg agcataacta cgccgagatc
     1681 gcgagcagat acctgccgat gccgattccg acagtcgaag ggattcgtac cattctgagc
     1741 gagcttgcga gtaccacgcc ggcagcaaag aatgccgatc cggagcaatt cgtctcatac
     1801 aagatcgcgc gggaaatcga agcgagcggc ttcgtcaaac ggctgtacga aaaataaggg
     1861 tggcgcttac cgacactaca atctcgatct ttcagccaag gagctactga tgacgaccga
     1921 ctatacaaaa aaacttctgc cccgttttcg ttacggagtg attcagccgc gcgcccacga
     1981 agaagttcag cgtgcgcgca gctatcagct ctatcgcttg ctgccgctcg agttcatgga
     2041 aatctcgacc ggccttggct tggagaatta caccaaggaa ggtgtcgaga aggcgatcaa
     2101 caattattgg gcgtgcgtcg atcggctggc taaagagaag gtcgatcata ttatcttcag
     2161 cggcgcgccg atctccgcga tgctgagtcg gccgagggtg ctgcaactgc tgcgcgaaac
     2221 gaaagagaga accggaatat ccgccgacgc tccgcttgag gcgctgatcg cggcaatgaa
     2281 gcatctcggc ttgaccaagc ttgcggtcgg cagccgctgg gccgacccgg tgaacgatgc
     2341 catcaaacgc taccttgaag ccggcgacat cgaggtcgtc ggcatcacca agcgaaacca
     2401 gtgggcgtcg gactcgttcg cgatgacact ggaacaaggg ttggaaatgg ccctcgatgt
     2461 cggtcgtgag gcggcaagga cctggcccga ggccgaagcg atctcggtcc cgggcggcgc
     2521 ggcgatgtca ctgcacgtaa ttccggcgat cgaggaagaa tacggcaagc cggcttttac
     2581 caacatgagc gccgaagtat ggaacgatct cgtgcgcccc ggcatcattg cgcccgtgca
     2641 aggctgggga cggctcctgg cgggcatgaa gtgagggtgc tcactttctc cgtttggtcg
     2701 aaatgacaat gacgggattt acctttgtga aactgtgagt tcagttcagt cgtggcgcgc
     2761 ggggagcttg tggtttagct atagaggcca gtggtgttag cgcggtggca cgacttcgat
     2821 caatatcgct tccgggtctg cgccccagcg aaagggcagc atcacactgc ccaacccgcg
     2881 tgagaccacc agcaatgtcg agccgtaagc gtacacacct tcgtcgagac agtaacggct
     2941 ttggcggata attgccggac cgcggggaaa acgaatctga ccgccgtgcg tatgacccga
     3001 caccatcaat ggtatgccgc gctgttcggc ttcgtaaaaa aaatccgggt tgtgggccat
     3061 cagcaaatgg ggtcgttgct gcgcggtcaa actctgccaa tcgggctttc ccataatacg
     3121 gtcatcaatg ccgccaagaa cgagcgcttt gccgtgatgc agcagggaaa cactctggtt
     3181 cttgagtgtc tggatgccga tacccgcgag attttccgtg agctcaacgg gatcgccgcc
     3241 gaagtaatcg tgattgccca agcagaacca tgccccgagc ggagcgttgg acaattgccc
     3301 caacccgtcg aaataaggga tcgcttcgtt gacatggccg ctgaccaaat cgccgccgat
     3361 cactaccaaa tcgggcttca tctgcatcaa agcgcgtagg atgccgctca gcgtctgcgg
     3421 ttttagaaaa atcccgccat gaatatccgt gatcagaagc acgcggagcg gcgcgaggtc
     3481 gttcggccaa cccaacggag caaccgtggt ttctgcaagg ctgaagcgct gctctaaaac
     3541 ccagcagtac gggttccaga aatttccgag gaacgggtag ataaagcgcg aaaacaaaat
     3601 gtcggtgtaa cgctcagcga ccttgatgac gccgcgattg ggattgatgc ggcgccgctc
     3661 tttacctgtc atttgccgcg gcggcggcag tactcgccgt ttaagcagcg taagctgatt
     3721 ccggggtttc atggtggggg cgcgcatttg tgaaaatatt gtagcgcgcc gtcgcgataa
     3781 atgcatcgat aaaggagcaa aactgtttcg ggtgttctcg gtcgaatcga tgaacgcggt
     3841 tcgccgatta gcgcgattgt catcgagcca ggcattcaca ttaattcaag aacttccaaa
     3901 gacaggattc gagtcagaaa gtaaattccc gggcattgat caagcacagc tatccgaagc
     3961 ttgactttgt tacgatgaat cccctatttt cgctcgcatc tcgaggcatc cacttaaggg
     4021 agatcacatg ctgatccatt cgaagtgttt catcgcgctt agattgccga tcgcgctcgc
     4081 gacagttttg ttgctgctag tttttgtcgt gagcgaagcg gcggaaagtc cccgacccgg
     4141 cggcgagctg gtatttgccg tgggcgagat tccaccgtcg tttgacggcc accgtgaaac
     4201 cacttttggc atgcttcatc cggttgcacc gcactacagc acgttgctgc gcttcgatcc
     4261 gcaaaattat ccaaaaatcg tcggcgatgt cgctgagaag tggtcatctt ccaaagacgg
     4321 tttgacggtg acggtgatga tccgtaaagg catcaaattt catgatggca cgccgctcac
     4381 ggcacaagac gtcaaggcaa cgtatgacaa aattattttt cccccggaag gagtagccag
     4441 cgcgcggaag gctttgtact ccatggtcga taaggtggaa gcaccggatg atgcgacggt
     4501 cgttttcaag ctcaagcaca tttccgcttc attcctggct aatctggcat cgccctggaa
     4561 cttcatctac agcgccgagc ggctcaagaa agatccgcgc tggtacgaga agaacgtgat
     4621 gggctccggc ccgttcattt ttgccgagca cgtcgccggc tcgcattggg caggtaagaa
     4681 aaatcccaat tactttctcc ccggacgtcc ttacctggac ggctttcgcg cggttttcat
     4741 ccgcgacacg gcgccgcgca tcgcggcggt gcgaagcggc caggcgatcg tcgagtttcg
     4801 cggcttcaat ccggctgctc gcgatgacat cgtaaaaagt ctgggcaaca aggtcgtcgt
     4861 ccaggagagt ccatgggcgt gcaatattct ggtcacgctc aacaatgaaa aaaagccgtt
     4921 caacgatgca cgcgtgcggc gcgccctgac gcttgccatc gatcgcaatg aagcgtcgaa
     4981 ggcgctgtcg cagatttccg tgatgaaata catcggcggt gtctttcgcc cg
//
LOCUS       DASULZ010000063         7044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_209631, whole genome shotgun sequence.
ACCESSION   DASULZ010000063 DASULZ010000000
VERSION     DASULZ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7044
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209631"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1682
                     /locus_tag="VFY96_01280"
     CDS             <1..1682
                     /locus_tag="VFY96_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEX5605113.1"
                     /translation="LREEQNGGEQIAFAYRGLDGVTRRTIVRCDPVPDSLTLAGAGFV
                     QSLGPKEEKEFALTYSCEIEGTTPVFIGSNYERSHAESERDLSVQQSHDAQISTSNEQ
                     FNDWLAQSTADLHMMFTSTPYGLYPYAGVPWFSTPFGRDGIITALEYLWVNPNVARGV
                     LSYLAATQARREDPERDAEPGKILHETRKGEMAALREIPFDRYYGSVDATPLFVLLAG
                     AYFEATQDLEFIKSIWPQINLALRWIDHYGDRDGDGFIEYARSSSRGLVHQGWKDSWD
                     SVSHSDGGLAHGPIALCEVQGYVYGARLAGATIATALARRKKARTLMARAEALKEKFH
                     ADFWCEDLLTYALALDGDKQQCRVVSSNPGHCLLTGIADPESAKSIARILMQENSFTG
                     WGVRTLAFSEARFNPMSYHNGSVWPHDNALIAAGLARYGFKEEASRILGGLFDASLFV
                     EYRLPELFCGFYRRKGAGPVAYPTACSPQAWSAASVFLLLQAILGMSIDAARSRLTFV
                     RPVLPSFLDEVRIANLRVGAASVDVLITRRPQYATVQVERREGRVELLTEA"
     gene            complement(1726..2160)
                     /locus_tag="VFY96_01285"
     CDS             complement(1726..2160)
                     /locus_tag="VFY96_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011305364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605114.1"
                     /translation="MSQPSDMERVRANTPAEINWRIDRQIEDSVRRYASEPKDVILRR
                     IWELEREWDIERVLQLMASSFSLTGILLSGIRDKRWIVLPAVVLSFLFLHAVQGWCPP
                     VPVLRRLGVRTREEIDRERYALKVLVGDFPTIANLQPGAATH"
     gene            complement(2386..2619)
                     /locus_tag="VFY96_01290"
     CDS             complement(2386..2619)
                     /locus_tag="VFY96_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX5605115.1"
                     /translation="MPLYEYECHNGHRFETIQKTSDKPLRRCSSCTARVKRAISQPTL
                     LHNRGVHVFDRVTKDDTIRDRPSSLKSLRKDKF"
     gene            2780..4246
                     /gene="guaB"
                     /locus_tag="VFY96_01295"
     CDS             2780..4246
                     /gene="guaB"
                     /locus_tag="VFY96_01295"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX5605116.1"
                     /translation="MLEQEVLPVGLTFDDVLLIPAESAVLPRDIDVSTRLTDRITLKI
                     PLVSAAMDTVTESNTAIAMAQEGGIGIIHRNLSVWAQAAEVEKVKKYESGMITDPITV
                     APDQKISEAQAIMNQYGISGLPVTKNGKLVGILTNRDLRFEKRHDRPVSEVMTKHNLV
                     TVKPGVDLDTAKELLHQHRIEKLLVVDDRLELRGLITVKDMEKKIQFPNASKDDRGRL
                     RVGAAVGVGPDWEERVETLVRAGVDLIAVDTAHGHSKNVLEVIGQIHRRYPDLDLAGG
                     NVATAEGTTALIQAGVNAVKVGVGPGSICTTRVVSGVGVPQITAIGSCAKAAARHNIP
                     IIADGGVKYSGDITKGLAAGADSVMIGSLFAGTEESPGEMILYQGRSYKAYRGMGSLG
                     AMGQGSRDRYGQADVKDANKLVPEGIEGQVPYKGSLSFNIHQLVGGLRAGMGYLGCPD
                     VAALHSKARFMQVTAAALREGHVHDVFITKEAPNYRVE"
     gene            4243..5775
                     /gene="guaA"
                     /locus_tag="VFY96_01300"
     CDS             4243..5775
                     /gene="guaA"
                     /locus_tag="VFY96_01300"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX5605117.1"
                     /translation="MILVLDFGSQYTQLIARRVRESKVYCEIHPFNIALEKIRQLQPE
                     GIILSGGPASVYQENAPTVSEDLFKMPVPVLGICYGMGVVNLAFGGEAGRAEQREYGP
                     ADLVIDDNADLFYGFSANEATRVWMSHGDKMTALPKGWSVLAHSGNSPIAAFANPTRC
                     YFGVQFHPEVMHTARGRQILDNFVFRVCACRPDWTMEHFIESSVSKIRNQVGDGRVLC
                     ALSGGVDSAVAATLVFRAIRERLICVFVNNGLLRKNEAERVCSLFVERFGRSFRYVDA
                     SEQFLDALQGVEDPEIKRKRIGNKFIEIFEHEARTLGDIEFLAQGTLYPDVIESVSVL
                     GPSATIKSHHNVGGLPTNMKFQLIEPLRELFKDEVRNVGKELGLPPEMIKRQPFPGPG
                     LAIRVLGEVSDGRLKILRDADAIVLDEVRAAGFYDKLWQSFAVLLPIKTVGVVGDERS
                     YENVIALRAVDSQDGMTADWVPLPYELLGKISNRILNEVRGVNRVVYDISSKPPATIE
                     WE"
     gene            5840..6667
                     /locus_tag="VFY96_01305"
     CDS             5840..6667
                     /locus_tag="VFY96_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010044526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX5605118.1"
                     /translation="MSRKEDEVLTAEEWGSTPGAPEVVRFLEGPQKRSIELVRAVKIF
                     CEFMRGFRALHFVGPCVTVFGSARFTERHPYYSVARECGVELAKAGFTVMTGGGPGIM
                     EAANRGAKDIGGRSIGCNIELPKEQKPNPYLDQWVTFRHFFVRKVMLVKYSYAFIALP
                     GGFGTLDEIFEAATLIQTAKISRFPLVLMVSEFWRPLLDFLSRHLVKEKTIDQADVDR
                     LVVTDSPRDAVAFITEIAMGQFGLSYGSRTKSRWYFGERQQSDPSSLIANRATRTDD"
     gene            6660..>7044
                     /locus_tag="VFY96_01310"
     CDS             6660..>7044
                     /locus_tag="VFY96_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX5605119.1"
                     /translation="MIEIAQVLLFDRHNRLLIYLRDDKAGIPFPNHWDFFGGHLEPGE
                     TPEQALVREVKEELGIELISWQFFRLYQCLQGDVYPNKKYIYRARIDRTIDELTLHEG
                     QRLASIELDQCFDFKFANILATILKD"
BASE COUNT         1554 a         1861 c         2033 g         1596 t
ORIGIN      
        1 atttacgcga agagcagaat ggcggcgagc agatcgcctt cgcttaccgg ggactcgacg
       61 gtgtcacgcg ccggacaatc gtacggtgcg atcctgtgcc ggacagtttg acccttgccg
      121 gcgccggttt cgtgcaatct ttgggaccca aagaagaaaa agagttcgcc ctgacttact
      181 cctgcgaaat cgaagggacg acgccggttt tcatcggttc gaattatgaa cgctctcacg
      241 cggaatcgga gcgagatctt agcgtacagc agagtcacga tgcccaaatc tccacgtcca
      301 acgagcaatt taacgactgg ctggcgcagt cgaccgccga tctgcacatg atgtttactt
      361 cgacgcccta cggcctctat ccgtacgcgg gagtcccatg gttcagcact ccattcggtc
      421 gcgacggcat catcacggcg ctcgagtatt tgtgggtcaa tccaaatgtt gcgcggggag
      481 tgctttcata tttggctgcg actcaggcac gacgagaaga tccagagcgg gacgcggagc
      541 cgggaaaaat tctccatgag acccgaaaag gcgaaatggc cgcgctacgg gagattccgt
      601 tcgatcgcta ttatggcagt gtcgatgcca cgccactttt cgtcctgctt gccggcgcat
      661 atttcgaggc gacccaggat ctcgagttta tcaagtcgat ctggccgcag atcaatctcg
      721 cgcttcgctg gatcgatcat tatggtgacc gggacggcga cggttttatc gagtatgcgc
      781 gatcgtcttc cagagggctg gttcatcagg gctggaaaga ttcctgggac tcggtctcac
      841 acagtgatgg tggcttggcg cacggaccga tcgcgctttg cgaggtgcag ggttacgtct
      901 atggcgcacg gcttgcgggt gccacgatcg ccacagcgtt agcgcgccgc aaaaaagcca
      961 gaacgctgat ggcgcgagcg gaagctctca aggagaaatt ccacgccgac ttttggtgcg
     1021 aggacctgtt gacctacgca ttggcgttgg acggtgataa acagcaatgc cgcgtcgtct
     1081 catcgaatcc cggtcattgc ttgctgacgg gtatcgctga tcctgaaagc gcaaaatcca
     1141 ttgcccgtat tctcatgcaa gagaactcct tcactggttg gggtgtgcgg acactggcgt
     1201 tttccgaggc gcgctttaat cccatgtctt atcacaatgg gtcggtttgg ccgcacgata
     1261 atgccctaat tgctgccgga ttggcacgat atggtttcaa agaggaggcg agtcgaattc
     1321 ttggcggctt attcgatgcg agcctttttg tcgagtatcg tctgcccgaa ctgttttgcg
     1381 gcttctacag gcgcaaaggc gccgggccag tggcgtaccc gaccgcttgt tcgccgcaag
     1441 cctggtctgc cgcatccgtg ttcctactat tacaagccat cttgggcatg agcatcgatg
     1501 ccgctcgctc gcggcttact tttgtgcgac cggtgctacc gagttttctc gatgaggttc
     1561 gaatcgcgaa cttgcgcgtc ggcgcagcct ccgtcgacgt gctgatcacc cggcgccctc
     1621 aatacgcgac ggtgcaggtc gagcgacgcg aagggcgggt agaactcctc accgaggcct
     1681 gatgagtgcc cgagctcggc tcctcgcgat ttgaggaacg caatgttagt gggtggccgc
     1741 gcccggctgc aaattggcaa tggttggaaa gtcgcccacc aaaaccttta atgcgtagcg
     1801 ttcgcgatca atttcttctc gagtccgtac acccaatcga cgcagcacag gtaccggagg
     1861 acaccaaccc tgcaccgcat gaaggaataa aaacgaaagt accacggccg gcaaaacgat
     1921 ccagcgttta tcccggattc cgctcaacaa gatcccggtg agtgaaaacg acgatgccat
     1981 cagctgcagg acgcgctcga tatcccactc acgttcgagt tcccaaatcc gacgcaagat
     2041 gacatccttg ggctcactgg cgtagcgccg aacgctatct tcgatttgcc gatcaatgcg
     2101 ccagttaatt tcggctggcg tattggctct gacgcgctcc atatccgaag gttgtgacat
     2161 aatgtggtcc tcctgtttca aagattcgct gtaaggagtg agcaagtgat atgccacgat
     2221 ttcctgaggt taaatcagga acaacgcttg tggcagcgag acaattgtcg gctgattact
     2281 gcgccagcag gaaaactacc gactgtggta ctcagcgcgt gtggcaatgc cgttcgcgtt
     2341 aagcccgtgg ccggaacgat ccctggccgg ctataccggc gtcgatcaga atttgtcttt
     2401 tcttagcgat ttcagcgagg atggacggtc gcggatagta tcgtctttcg taacccggtc
     2461 gaagacatgc acaccgcggt tatggagcag ggtcggttgc gagatggcgc gtttcacgcg
     2521 agcagtgcag ctggagcaac ggcgcagcgg cttgtcagaa gttttctgaa tggtctcgaa
     2581 gcggtgtccg ttgtgacact cgtattcata gagcggcatg ggcagttgct atcacaatgg
     2641 cccgtgcgcc tcaatgcgaa atcttgccgc ccgaaaaatt tcctggtata agatttcatg
     2701 tttgctcgaa taacagatga gcgccaaaaa acccaggcaa ggtctgggtt tttttttatc
     2761 tcaccccgga gggtgaaaca tgcttgaaca agaagtcttg cctgtcggtt tgactttcga
     2821 tgacgttttg ttgatccccg ccgagtccgc cgtattgccg cgcgatatcg atgtttcgac
     2881 ccgtttgacc gatcgcatta cgctcaaaat tccgctcgtc agtgcggcaa tggatacggt
     2941 aaccgagtcg aacaccgcca tcgccatggc acaagaagga gggatcggca tcattcaccg
     3001 caatttgtcg gtctgggcac aggcagcgga ggtggagaag gtaaaaaaat acgaaagcgg
     3061 aatgatcacc gatcccatca cggtagcgcc cgatcaaaaa atttccgaag ctcaggcgat
     3121 catgaaccag tacggtattt cgggattgcc ggttacgaag aatggcaagc tcgtgggaat
     3181 tctcaccaat cgcgatttgc gcttcgagaa acgtcatgat cgtccggtct ccgaagtgat
     3241 gacgaagcac aatctcgtga cggtcaagcc aggcgtcgat ctcgatacgg cgaaggagct
     3301 tttacatcaa caccggatcg aaaagctctt ggttgtggac gatcgcctcg agctcagggg
     3361 cttgattacc gtaaaggaca tggaaaagaa aattcagttc cctaacgcct ccaaggatga
     3421 ccgcggacgg ctgcgagtgg gagctgcggt cggagtcggg ccggattggg aagaacgtgt
     3481 cgaaacgctg gtgcgcgccg gagtcgatct catcgccgtc gacacggcgc atggccattc
     3541 gaaaaatgtg ctcgaggtca ttggacagat ccaccgacgc tatcccgatc tcgatcttgc
     3601 ggggggcaat gtcgccacgg cggaaggaac gacggccctg atacaggccg gcgtcaacgc
     3661 ggtaaaagtt ggcgtcgggc cgggctccat ctgcacgacc cgcgttgttt cgggagtcgg
     3721 cgtgccccag attaccgcga tcggcagttg tgcgaaggca gcggcgcgtc acaatatccc
     3781 catcattgcg gatggaggcg tcaagtattc cggtgatatt acgaagggac tcgcagccgg
     3841 cgcggacagc gtcatgatcg gcagtctctt cgcaggcact gaggaaagcc cgggcgagat
     3901 gattctctat cagggaagaa gctacaaagc gtatcgtggt atgggatcgc tcggagcgat
     3961 ggggcaggga agccgcgatc gttatggtca agcggacgtg aaagacgcca ataagctcgt
     4021 ccccgaggga atcgaaggtc aggtgccgta caaaggatca ctttccttca atattcacca
     4081 gctagttgga ggattgcgcg ccggcatggg ttatctcggc tgtccggatg tggcggcgct
     4141 tcatagcaaa gccagattca tgcaagtgac cgctgcggcg ttacgggaag gtcacgttca
     4201 cgacgtgttt atcaccaagg aagcgcccaa ctatagagtc gaatgatcct cgttctggac
     4261 ttcggttccc aatacaccca actcatcgcc cggcgggttc gcgagtcgaa agtctattgc
     4321 gagatccatc ccttcaacat tgcgctcgaa aaaattcggc aactccagcc ggaggggatt
     4381 attctctcgg gcgggccggc gagcgtatac caggaaaacg ccccgacagt cagcgaagat
     4441 cttttcaaga tgcctgtacc cgttctcggc atctgttatg gcatgggcgt cgtcaatcta
     4501 gctttcggtg gagaggccgg ccgcgctgaa cagcgtgaat acgggcccgc tgatctcgtg
     4561 atcgacgaca acgccgatct tttttacgga ttctccgcaa acgaagcgac ccgcgtctgg
     4621 atgagccatg gcgacaaaat gaccgcgttg ccaaagggct ggagtgtgct cgcgcacagc
     4681 ggcaactcgc cgattgcggc ttttgccaat ccaacccggt gctacttcgg cgtacagttt
     4741 catccagaag tcatgcatac agcgcgtggg cggcagattt tggacaattt cgtattccgg
     4801 gtttgcgcct gccggcctga ctggacgatg gagcatttca tcgagagctc ggtcagcaaa
     4861 atacgcaacc aagtcggcga cgggcgcgtt ttgtgcgcgc ttagcggcgg ggtcgattcc
     4921 gctgttgcgg ccaccttggt tttccgcgcg attcgcgagc ggctgatttg cgtttttgtc
     4981 aataatgggc tgctgcggaa aaacgaagcc gaacgcgtct gcagtttgtt tgtcgaacgg
     5041 tttggccgga gctttcgcta tgtcgatgct agcgagcagt ttcttgacgc gcttcaaggc
     5101 gtcgaagatc cagagatcaa acgaaagcgc atcggcaata agttcattga aatcttcgag
     5161 cacgaagcgc gtacccttgg ggacatcgaa ttcttagctc aggggacgct ctatcctgac
     5221 gtgatcgaat cggtctccgt gctcggcccc tctgctacga tcaagtcgca tcacaacgtc
     5281 ggtggtctgc cgacgaatat gaagtttcag ctcatcgaac cactccgaga gctattcaag
     5341 gatgaagtta gaaatgtggg caaagagcta ggtttgccgc cagaaatgat taaacggcag
     5401 ccttttcccg gtcctgggct agcaatccgt gttttgggcg aagtcagcga tggacggtta
     5461 aaaattctgc gcgacgccga tgcgatcgtc cttgatgagg tccgggctgc cggattttat
     5521 gataagctct ggcaatcgtt tgcggtgttg ctgccgatca aaacggtcgg cgtggttggc
     5581 gacgagcgca gttacgaaaa cgtgatcgcg cttcgagcag tggatagcca ggatggcatg
     5641 accgccgatt gggtgccctt accttacgaa ctcctcggca agatttcgaa ccgcattttg
     5701 aacgaggtgc ggggcgttaa tcgcgtcgtt tacgatattt cctcgaaacc gccggcgaca
     5761 atcgagtggg aatagatttc gtcctgcgat agttgagttg ggacagtgtg ctcactcgct
     5821 ggagcgtaac aatttttcca tgagcagaaa agaagacgaa gtcctcacag ccgaggagtg
     5881 gggctccact ccaggcgcgc ctgaagtcgt gcgttttctc gaaggaccgc agaaacgcag
     5941 cattgagctt gttcgcgccg tgaaaatttt ctgcgaattt atgcgcgggt ttcgcgcgct
     6001 tcatttcgtc ggcccttgcg tgactgtttt cgggtcggcg cgattcacgg aacgacatcc
     6061 ctattacagc gttgcgcgtg agtgcggcgt ggaactcgcc aaagcgggtt tcacggtgat
     6121 gaccgggggc gggcccggca ttatggaagc cgccaatcgg ggcgcaaaag atatcggcgg
     6181 tcgatcgata ggctgcaata tcgaactgcc gaaagaacaa aagcctaacc cgtatctcga
     6241 tcaatgggtc acgtttcggc attttttcgt gcgcaaagtc atgctggtca aatattccta
     6301 cgctttcatc gctttacccg gcgggtttgg cactttggac gagattttcg aggccgcaac
     6361 tttgatccag accgcgaaga tcagtcgctt cccgctggtt ttgatggtca gtgagttttg
     6421 gcgcccattg ctcgacttcc tctcgcggca tctggttaag gagaaaacca tcgatcaggc
     6481 tgacgtagat cgcttggtcg tcactgactc gcctcgtgac gccgtagcgt ttatcactga
     6541 gatcgccatg ggccaattcg gtttgtccta cggttcgcgc acaaagagcc gttggtattt
     6601 tggggaacgc caacagtctg atccctcgtc attgatcgcc aatcgagcca ctcggaccga
     6661 tgattgaaat cgcccaggtc ctgctcttcg atcgccataa ccgtttgctc atttatctgc
     6721 gcgacgacaa ggcaggtatt cctttcccga atcactggga ttttttcggc ggccacctag
     6781 aaccaggcga gacgcccgag caggcactgg tgcgcgaagt gaaagaggag ttagggatcg
     6841 agcttatcag ctggcaattt ttccgtttgt atcaatgcct gcaaggagac gtttacccga
     6901 acaaaaaata tatttaccga gcgcgaatcg atagaaccat tgatgaattg acgctgcacg
     6961 aaggacaaag actcgcgagc atcgagctcg atcaatgctt tgatttcaaa tttgcgaata
     7021 tcctcgccac catcttgaag gatt
//
LOCUS       DASULZ010000064         3049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_7152, whole genome shotgun sequence.
ACCESSION   DASULZ010000064 DASULZ010000000
VERSION     DASULZ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7152"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1175
                     /locus_tag="VFY96_01315"
     CDS             132..1175
                     /locus_tag="VFY96_01315"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5605120.1"
                     /translation="MMKPQRNRPRIIDADGHIRETDEEIIEYMSPGYRARRDAMLYFP
                     LTPHHGWHRSIPANDFRQQDFRVPDWREWVSKLDEGGFELTVLYPTRFMHIGQIGNPP
                     YAVELCRAYNDFLYDRFLIRDQRFRGMALLPLQDPAAAVKELRRAVNNYDMLGGILPA
                     EGLPLPLGHRQYWPVFQEAEKLGCTLSIHSCNSLRDNDRYLVPNEAATLTHVIPQMRQ
                     FTNILFSGLMNKLKKLRLGFLEAGCGWVPFIMSKIEERLERVAPKERPILPSELLKRK
                     QIYFQCGEEITTKRDVELLGDDCLLWASDFPHEATRTDMRKLVKEHFARKDLNRDAKR
                     KIIYDNAKRFYGL"
     gene            complement(1211..2179)
                     /locus_tag="VFY96_01320"
     CDS             complement(1211..2179)
                     /locus_tag="VFY96_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605121.1"
                     /translation="MFLALALSHPAPAQDKRLERIRVGGGSTSATQMSMWLAKEGNLY
                     EKHGLSVEAISIPGSSLALQAMLAGELPIIQLGGAASIQANLAGADTVIIATIVKKFL
                     FWIYGRPEISRMEDLKGKVFGTTRFGTLSDLASRFALRFYGIDPERDITMVQTGGPAE
                     TVTAILSGKIHAAALSPPATLQAKKAKLKELLDMSKLDAEYHINGVVTTRRYLKTNED
                     TVRRFMRAYIEGATRAQKDKTFALKAMGRTFRTDDRELLDESYDMIIKSNFVIPPYPS
                     VPGIASLLKGLEQTNPKAKTAKPEDFADSRIVKELDQSGFVKAVMQ"
     gene            complement(2511..>3049)
                     /locus_tag="VFY96_01325"
     CDS             complement(2511..>3049)
                     /locus_tag="VFY96_01325"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5605122.1"
                     /translation="ERYHVVGNSVGGYIAARLALEDSRVDKFVSTTSGTLAPKGSTES
                     QALGQKHSDELREYVPSMENMRALTFGTLFRKDLVTVELVRARHEMSIGKNAEAARLR
                     RGIKAPRPLFGELPRLKKKTLLLWGAQDRGVSVERGILLFQLVPNAEFHLFDQCAHWV
                     QWDQAERFNRLVADFLKA"
BASE COUNT          719 a          885 c          777 g          668 t
ORIGIN      
        1 tataagacgc gcgatacagt gaaatgaaac aagaattcac aatcagaggt caatgataca
       61 gattgacaga ctgacaaagc ccatgcaaca aaatcaaaca aaccgtgtag gaaggcatat
      121 acaaggatga aatgatgaaa ccgcagcgca accgacctcg tatcatcgac gccgatggcc
      181 acatccgcga aaccgacgaa gaaatcatcg aatacatgtc ccccggctat cgtgctcggc
      241 gcgacgcaat gctttatttt ccattaaccc cgcaccacgg ctggcaccgc tcgatcccgg
      301 caaatgattt tcgccagcag gattttcgtg ttccagactg gcgcgaatgg gtcagcaagc
      361 tcgacgaagg aggtttcgag ctgacggtgc tatatccgac ccggttcatg catatcggcc
      421 aaatcggcaa cccgccctac gccgtcgagc tatgccgcgc ctataacgat ttcctctatg
      481 acaggtttct catccgtgat cagcgctttc gtggtatggc gcttctgccg ttgcaagatc
      541 cggccgccgc cgtcaaggag cttcgccgcg ccgtcaacaa ttacgacatg ctcggcggca
      601 ttctgccggc ggaaggattg cctctgcccc ttgggcaccg gcagtattgg ccggtttttc
      661 aggaggccga gaagctcggc tgcactctat cgatccactc gtgtaattcg ctgcgtgaca
      721 atgaccgtta tctggtgccg aacgaagcgg cgacattgac ccacgtcatt ccgcagatgc
      781 gccaattcac caacattctg ttctcggggc tgatgaacaa actcaaaaaa ctccggctcg
      841 gctttcttga agcgggttgt ggttgggtac catttataat gagcaaaata gaagaacggc
      901 tcgagcgtgt ggcgccgaaa gagcgtccaa tactacccag cgagctcctg aagagaaaac
      961 agatctattt tcaatgcgga gaagaaatca ccaccaaacg cgatgtcgag ctgctcgggg
     1021 acgactgcct gctctgggcc tccgatttcc cccacgaagc gacccgcacc gacatgcgca
     1081 agctcgtcaa agaacatttc gcccgtaaag atttgaaccg cgacgcaaaa agaaaaatca
     1141 tctatgataa tgcgaagcgg ttctacggac tgtagacacc agccgcacaa agaacgttca
     1201 gatttaaccc tcactgcatc accgctttga caaacccgct ctgatccagc tcctttacga
     1261 tccggctgtc cgcgaaatct tccggctttg cggtttttgc cttgggattg gtctgctcca
     1321 atccctttaa caaacttgca atccccggca ctgagggata cggcgggatc acaaaattgc
     1381 tcttgatgat catgtcataa ctctcgtcga gcaactcgcg atcgtccgta cgaaacgttc
     1441 ttcccatcgc tttgagcgca aacgttttat ctttttgagc acgcgtcgcg ccttcgatgt
     1501 aagcccgcat gaagcgcctg accgtgtctt cgttggtctt taaataacgc ctggtcgtca
     1561 ccaccccatt gatgtgatac tccgcgtcga gcttcgacat atcgagcaat tccttgagct
     1621 tggctttttt cgcctgcaac gtcgccggcg ggctcagcgc cgccgcgtga atttttccac
     1681 tcagaatcgc agtcacagtt tccgccggcc cgccggtttg caccatcgtg atgtcgcgct
     1741 ccggatcgat gccataaaac cgtaacgcaa atcgtgaagc gagatctgag agcgtgccga
     1801 accgggtggt gccgaacact ttgcccttca aatcttccat ccggctgatt tctggccggc
     1861 catagatcca gaagagaaac ttcttcacga tcgtcgcaat aatcacggta tcggcgccgg
     1921 caagattcgc ttggatggat gcggcgccgc cgagctgaat gatcggcaat tcgccggcaa
     1981 gcatcgcctg caacgccagc gagcttcctg ggatactgat cgcttcgacg ctcaatccgt
     2041 gcttctcgta gaggttacct tccttcgcaa gccacatgga catctgcgtc gcggaagttg
     2101 acccgccgcc gacacggatt cgttccaatc ttttgtcctg agccggtgcc ggatgcgaca
     2161 atgcaagcgc taggaacatt actgtgccgg caaccgatcg tattcggatt atgcggtctc
     2221 gatatttatg aagaagtctc atgacgcgcg agattagcga gcgacacccc gcagagtcaa
     2281 gcctgagcgg gagtgctcgg atcggacact ctactgtcct gggctgaaat cagaatggaa
     2341 accagctgga taccggtcct cgccggttga cgatcgccca gatccaggac actaatagga
     2401 gagctgggat gaacccgagt atcggtaaca agagtcgaga aacgacggct ccaatcaggt
     2461 ccggttctct cacatatttg gcaaatagcg aggtgcagat ttttgcgcaa tcacgctttg
     2521 agaaaatccg cgaccaatct gttgaaccgt tctgcctgat cccattgcac ccagtgggcg
     2581 cactgatcga acaggtgaaa ttcggcattg gggacgagtt gaaagagcag gatgccacgc
     2641 tccaccgaca caccgcgatc ctgagcgccc cacaggagta gcgtcttctt cttcaatcga
     2701 ggtaattctc cgaagagcgg ccgtggagct ttaatgccgc ggcgcaatcg ggcggcctcg
     2761 gcattttttc cgatactcat ctcgtggcgc gcgcgcacca actccaccgt caccagatcc
     2821 ttacgaaaca acgttccgaa cgtaagcgcg cgcatgttct ccatgctcgg gacgtattca
     2881 cgtaactcat cagaatgctt ctgtcccagt gcttgcgatt ccgttgaacc tttgggcgcc
     2941 agtgtaccgc tggtagtcga aacgaactta tcgacccggg agtcttccag cgccaggcga
     3001 gcggcgatat agccgccgac ggaattgccg accacgtgat agcgctcga
//
LOCUS       DASULZ010000065         2581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_412926, whole genome shotgun sequence.
ACCESSION   DASULZ010000065 DASULZ010000000
VERSION     DASULZ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412926"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VFY96_01330"
     CDS             <1..310
                     /locus_tag="VFY96_01330"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5605123.1"
                     /translation="LREMKATSFLINTARGELVHEEALHDALMDGRIGGAGLDVMEHE
                     PPKSDHPLRFSECVVITPHCAWYTNRSQMELRQKACAEVIRVLRGGVPKHLVNKIALR
                     "
     gene            433..1461
                     /locus_tag="VFY96_01335"
     CDS             433..1461
                     /locus_tag="VFY96_01335"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5605124.1"
                     /translation="MKSKRLMLACAILIGVAFAARAQGQSKDPLLDAAKKEGKVVFYS
                     SEDRTALAEPVKAFEAKYGIPIELNRQSTGRILKVAQSEFETNTVRSDLLDITNPLVF
                     FDWKKKGYLKQWKASHANEQVSDVFRDGEDFIWSRRLLISCIGYNKKALSEAEAPKSW
                     QDLLTAKWKSKVASAGPEAGPTFGWIVYISRKYGWDYFDKLHANDTIVAPSSDETVAL
                     LATGERPLAVVNCFDILEAGAQGSPIANIFPTDGLAPWWAGLAIPKAAPHPNAALLLA
                     NFVMSAEGAAAASKGRHGYVAYLNKSTPMAKVLPPIEDLVKGYPKIDWATLNPADITK
                     EFRKRMGQ"
     gene            1694..>2581
                     /locus_tag="VFY96_01340"
     CDS             1694..>2581
                     /locus_tag="VFY96_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5605125.1"
                     /translation="MAERANRTISLWRWNNNGLPSATAGETPLRRLSRCLPVVLPLLV
                     LAVLVVYPVAGLVGSSFFDEKGFTVGNYTQLLTTRFFNLMVNTMVLGLGSAMLATAAG
                     TMFAWLCVCTDLPFRKAFTILHIAPYGLPTIAAALAWIFLLTPKAGLFNRFLMWLFGL
                     QNAPFNILSMPGMIWALAAHLYPWAFLLTSAAFATMDRSLEDAARMSGASEFKTFRKI
                     TVPLVAPSIAAAFILVFTISIEQFSIGGTIGVLARIPILPVEIYRSIAVYPPEWGFAT
                     TLSVVLLLITSLLIYGQNKI"
BASE COUNT          582 a          704 c          713 g          582 t
ORIGIN      
        1 gttgcgcgaa atgaaagcca cgagtttttt gatcaatacg gcgagaggag aacttgtcca
       61 tgaagaagcg ctgcacgacg ccctcatgga cgggcggatc ggcggcgccg ggctcgacgt
      121 catggaacac gagccgccaa agagcgatca tccgctcaga ttctcagagt gcgttgtcat
      181 cacgccccat tgcgcctggt atacaaaccg atcccaaatg gaattgcgtc agaaagcgtg
      241 cgccgaagtc attcgcgtgc tgcgcggcgg tgtgccgaag caccttgtga ataaaatagc
      301 tttgcgatag atagaaacgc gccagaaatc ctgttgctcg gaaacgggtg ggattaattc
      361 aattaggatt cgtgatctca ctggtactga actgaatgga tgctttgaga cttccgcgat
      421 aaggagagtc ctatgaagag caaacgtctg atgctggctt gcgcgatttt gattggagtg
      481 gctttcgctg cgcgagcgca aggccagtcg aaagatccac ttttagatgc ggccaaaaaa
      541 gagggcaagg tggtttttta ttcctcggaa gaccgcaccg ccctcgcaga gccggtcaaa
      601 gcttttgagg ccaaatatgg cattcccatc gagctcaacc gccagtcgac cggacgcatt
      661 ctcaaggtcg cccagtccga gttcgaaacc aatacggttc gttcggacct cctggatatc
      721 acgaatccac tcgtgttctt cgactggaaa aagaagggct atttgaaaca atggaaggca
      781 agtcatgcaa acgagcaggt ttcggatgtg ttcagagacg gggaggactt catctggtcc
      841 agaaggttac tgatcagctg catcggttac aacaagaaag ccctcagtga ggcagaagcg
      901 ccgaaaagct ggcaagatct cctcacagcg aagtggaaaa gtaaagttgc cagcgccggt
      961 cccgaagccg gaccgacctt cggctggatc gtctacataa gccgtaaata cggctgggac
     1021 tatttcgata agctgcacgc gaacgatacc atcgtcgcgc cgagcagcga cgagaccgtt
     1081 gctttgttag cgacgggcga acgaccgctc gcggttgtga attgtttcga catcctcgaa
     1141 gctggggccc aaggctcgcc gatcgccaac atctttccca cagacggcct cgcgccctgg
     1201 tgggccggac tggcgatccc gaaggcggct cctcatccca acgcggcgct tctgctcgcg
     1261 aatttcgtga tgagcgccga gggagcggcc gctgcctcca agggaagaca tggttatgta
     1321 gcctatctga acaaaagtac tccgatggca aaggtcctgc ctcccataga agacctggtg
     1381 aaaggctacc caaaaatcga ttgggctacc ctcaaccctg cggacattac gaaagagttt
     1441 cgtaagagaa tggggcagta atcacgagcg aaccctggat agccgcaagg tcggggtgat
     1501 ctgatttggt gacacccccc atctcgatct tccccccgtc gagggggaag aggtctgaat
     1561 cgaatgcagc gatatgcggc tcccattctt ttctttgtgg gcagcgtaag cttatgaatc
     1621 gtcgatggaa cggggttcga tcactctgct agatggatga gagtctgaat catccgcata
     1681 gttggcacct gctatggcgg aacgcgcaaa ccgaacgatc tcgctctggc gatggaacaa
     1741 taacgggcta ccttcggcga cggcaggcga aacaccgctg cgccgacttt cccgctgtct
     1801 tccggtcgtt ctcccgctcc tcgtcctagc cgtgttggtc gtttatcctg tcgcagggct
     1861 cgttggctcg agtttcttcg atgaaaaagg atttacggta ggcaattaca cccagctcct
     1921 gacgacgcgc ttcttcaatt tgatggttaa caccatggtc ctgggtctcg gctcggccat
     1981 gctcgcgact gcggcgggca cgatgttcgc ctggctgtgc gtttgcacgg acttaccgtt
     2041 tagaaaagct ttcacgatac ttcatatcgc gccttacggt ttgccgacga tcgctgccgc
     2101 tctcgcctgg atatttctgc tcacgccgaa agccggtctc ttcaaccggt ttctgatgtg
     2161 gcttttcggc ttacaaaatg cgccctttaa tatattgagc atgcccggca tgatctgggc
     2221 gctcgctgct catctgtatc cgtgggcatt cctgctgaca tcagcagcgt tcgcgacgat
     2281 ggaccgatca ctcgaagacg ccgcccggat gagcggcgca agtgaattca aaacttttcg
     2341 aaagatcacc gtccccctgg ttgcgcctag catagccgcg gcattcatac tggtgtttac
     2401 gatctcgatt gagcagttct cgatcggagg caccatcggg gtgctcgccc gaattccgat
     2461 cctgcctgtc gaaatctacc gctcgattgc ggtctatcct ccggaatggg gatttgccac
     2521 cacgctctcc gtggtgctgt tgttaatcac cagcctgctt atctatggac aaaacaagat
     2581 a
//
LOCUS       DASULZ010000066         2214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1326417, whole genome shotgun sequence.
ACCESSION   DASULZ010000066 DASULZ010000000
VERSION     DASULZ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326417"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /locus_tag="VFY96_01345"
     CDS             <1..1252
                     /locus_tag="VFY96_01345"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX5605126.1"
                     /translation="IVVLMMENRSFDHMLGYLRLEENRNDVEGLTPGLSNSDGQKSYF
                     IHHLDHTALKTSQDPCHSRQCVADQLQNNNGGFVSNYVSTHPNDPERDLVMGYYNKNE
                     LTTYDYLAAEFAVCDHWFSSVPGATWPNRLYAVTGRADGSKDNKTIPIYNLPAFVRKL
                     DAKKVTWDWYSDNFFGSTLNLTDGRYRSRSVYHLEDFYQQAADGTLPSVCWIDPLFIN
                     SPGVIAANDDHPPADVRAGQDFVLNIYHALVSNPVQWNKTLFLLVYDEHGGFYDHIVP
                     PAAEDNRPSFRSYGARVPALLVSPWVPQGKAVSNLFDHTSIIKTILLRFCRAADGTIP
                     DMGKRVMAANHLGYALTEATPRPSPSKENYDHVVADLADWHAERFEARFQPDHVRTNQ
                     PHELNDFQKGLLRASERLPTRREH"
     gene            1496..>2214
                     /locus_tag="VFY96_01350"
     CDS             1496..>2214
                     /locus_tag="VFY96_01350"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5605127.1"
                     /translation="MAVIVSIVAAVFLSVVSSQANAASAEDFYKGKTIRVVVGFAAGG
                     GFDSYARILARHMPKHTPGNPALIVDNMAGAGSRLAANYLYKASAPDGLTIGNFIGSL
                     IMQQVLGDQSVEFDGRKFEWLGAPVTDETACALTTASGVTNMDQWLAAKKPVKLGGEA
                     PGANDSDVPRVMRAALGLPIQLIEGYKGTSNIRLAAESGEVDGGCWTWSSIRSTWKKG
                     IESGAVKVVLQVNAKKAADLP"
BASE COUNT          499 a          608 c          623 g          484 t
ORIGIN      
        1 gatcgtcgtg ctgatgatgg agaaccgctc cttcgatcat atgctcggct atctcaggtt
       61 ggaagaaaac cgaaacgacg tggaaggact gacgccgggc ttgagcaaca gcgatgggca
      121 gaaaagctat ttcatccatc atcttgatca caccgcgctc aaaacaagcc aggacccgtg
      181 tcatagcagg caatgcgtcg ccgatcagct gcaaaacaac aacggtgggt tcgtcagtaa
      241 ttatgtgagt actcatccga atgatcccga gcgggacctg gtgatggggt actacaacaa
      301 aaatgagctc accacgtacg actatctcgc cgccgaattc gccgtgtgcg atcactggtt
      361 ctcttccgtt cccggtgcca cctggcccaa tcggctgtat gcggtcaccg gacgcgctga
      421 tggcagcaaa gacaataaaa cgattccgat ttacaacctt ccggctttcg ttcgcaaact
      481 cgatgcaaaa aaggtgactt gggattggta tagcgataac ttcttcggtt cgacgctaaa
      541 tctgaccgac ggccgctatc ggtcgcgcag cgtttatcac ttagaggatt tttatcagca
      601 ggcggccgac ggtactctgc caagcgtctg ttggatcgat ccgctcttca tcaatagccc
      661 cggcgtgatt gcggccaacg acgatcatcc tcccgccgac gtccgggccg ggcaggactt
      721 cgttcttaat atctatcacg cgctcgtcag caatccggtc cagtggaaca agacgttgtt
      781 cttgctggtt tatgacgagc acggcggctt ttatgaccat atcgtcccgc ctgccgcgga
      841 agacaacagg ccaagtttcc ggagttacgg cgcgcgtgtt ccggcgcttt tagtctctcc
      901 gtgggtgccg caaggaaaag ctgtgtcgaa cctattcgat cacacgtcga tcattaaaac
      961 gattttgctg cgcttctgtc gtgccgctga cggaaccatc ccggatatgg gaaaaagagt
     1021 catggcagca aatcacttgg ggtacgcctt gaccgaggca acgccgcgac catcgccgtc
     1081 aaaggagaat tatgaccacg tcgtcgccga cttggcggac tggcatgccg aacgtttcga
     1141 ggcgcgattt caacctgatc atgtcagaac caatcagccc catgagctca atgattttca
     1201 aaaaggtctc ctgagagctt ccgagcgatt gccgactaga cgcgaacatt agttcaatgg
     1261 cgacgcgacg atccatgcac cggtaagatg aagaaccggc gcgatggctt gcagtcagat
     1321 cgatcaaccc tgtcaggctg cttcatcgag cgaaacgggt gacgattttc gaaccgacag
     1381 ccagccggta ttgcctctat cgatgggagt cgtgcggatc tacagatttc tcttttcaaa
     1441 cccctccttt tggactaaac atcgccagca agctttcatc atcagaggag gagtcatggc
     1501 cgtgatcgtt tctattgtcg ctgctgtctt tctgagcgtc gtctcgtctc aagccaacgc
     1561 cgcttcggcg gaggattttt acaagggtaa gaccatccgc gtcgttgtcg ggtttgccgc
     1621 cgggggaggg ttcgatagtt acgcgagaat cctcgcgcgc catatgccga agcatacgcc
     1681 cggcaatccg gcgctgattg tcgacaacat ggcgggcgcg ggcagccgtc tggcagcaaa
     1741 ttatctatac aaagccagcg ctcccgatgg cttgaccatt gggaatttca tcggctcgct
     1801 catcatgcag caggtcctcg gcgatcagag cgtggaattc gacggtcgta aattcgaatg
     1861 gctcggcgcg ccggttacgg acgagacggc atgcgctctc acgacggcca gcggtgtcac
     1921 caatatggat cagtggctcg ccgcaaaaaa accggtgaag cttggcggcg aggctccggg
     1981 tgcgaacgat tccgacgtcc cgagagttat gcgggcggcg cttgggctac cgatccagtt
     2041 gatcgagggt tacaagggca cttcgaatat tcgccttgcg gccgagtcgg gagaagtcga
     2101 cgggggttgc tggacctggt cgtcgatccg gtcgacctgg aagaagggaa tcgaatcggg
     2161 cgcggtcaag gtcgtcctgc aagtcaatgc gaaaaaggcc gcagatcttc ccaa
//
LOCUS       DASULZ010000067        13448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_7376, whole genome shotgun sequence.
ACCESSION   DASULZ010000067 DASULZ010000000
VERSION     DASULZ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 13448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13448
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7376"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /gene="nth"
                     /locus_tag="VFY96_01355"
     CDS             <1..659
                     /gene="nth"
                     /locus_tag="VFY96_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX5605128.1"
                     /translation="EKAHPDAKLDLDYKNPLELLIALILAAQARDDLVNQITANLFKI
                     QRSAADYANTPLTSLERQIGKINFYRTKARSIHNCCHHLVDRFDGKVPANLDDLLSLP
                     GVGRKTANILLGNAYGRQTIGVDTHVLRLSQRLGFTKNTDPDKIEADLMVIVPDKQRV
                     RFCHLLQYHGRRLCWAKKPNCPACTIRELCPFPHKTKSVVGAIRHSPRHPSKSKQQTG
                     "
     gene            776..1546
                     /locus_tag="VFY96_01360"
     CDS             776..1546
                     /locus_tag="VFY96_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605129.1"
                     /translation="MPDSFEAPDPSRLFLEARGLIELPLLLFRFPELAWQPRGEGRPV
                     LILPGYGAGDGSTAILKSYLKLLGYRARGWGLGRNTGTAADLLPRILKRVSSLARRTQ
                     QQVHLIGWSFGGYLARELARERADLIGQVITLGTPVIGGPKYTVVARSFHRRGIDMDA
                     IAAEVEMRNRITFTTPVIALYSRRDRVVAWQACIDANAQNVEHVEVRTTHFGFGFSPE
                     VYKIIAQRLAANRASATGRAQRELWRWRWPGASRQAKR"
     gene            1577..2065
                     /locus_tag="VFY96_01365"
     CDS             1577..2065
                     /locus_tag="VFY96_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEX5605130.1"
                     /translation="MDSAFFLWPIAGVLVLAGLAGLILPAIPGAPLIFAGLVVGALAD
                     DFVYVGAWTLIALAVLTLLIIGVDFWATMFGAKKFGASRRAVIGAVVGSIAGLFLGVP
                     GVLFGPFIGAVLGEISSAGSLRQATRAGFGATLGLILGAAMKLALAFSMIGIFVAVRF
                     IQ"
     gene            2149..3069
                     /locus_tag="VFY96_01370"
     CDS             2149..3069
                     /locus_tag="VFY96_01370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX5605131.1"
                     /translation="MKTLTVLFAALIIHGQALALTIATGSAEGTYFKIAQDIKQIAEK
                     EGIPIEVVQTNGSFDNINLLGAGKVDLAILQLDVLRFVAEIMLKETGFNVLQEAKVVL
                     NLYPEEIHIIAKDEKIRTVQELQGKRVAVGPEKSGSALTAEVLLAGFNVHIDRAFDTP
                     EEAIKKIVSGDLDALVFVGGAPVPAFRNLDPSFHFVTIPAESGLEQIYPKKKIEQNVY
                     SWAGEVETHAVPSVILTRNRDDRDFVVSLQKLVLSILVNKESLDANGHPKWKDSYVRS
                     TLGGVGYGPTTEVILLYNTLDSAGYRIIKK"
     gene            complement(3090..3482)
                     /gene="gloA"
                     /locus_tag="VFY96_01375"
     CDS             complement(3090..3482)
                     /gene="gloA"
                     /locus_tag="VFY96_01375"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010873329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEX5605132.1"
                     /translation="MKIVHTMIRVNDLDESLKFYCNGLGMKLLKRRDYESGRFTNVFV
                     GYGSEETDAVIELTYNWDTHTYDLGNGFGHIALGVPDVYKACEEFRAKGIKIVREPGP
                     MKHGSGSNAIAFLEDPNGYKIELTTRPD"
     gene            complement(3629..4600)
                     /locus_tag="VFY96_01380"
     CDS             complement(3629..4600)
                     /locus_tag="VFY96_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003934011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5605133.1"
                     /translation="MDHGVGLFPTEPLQKMIHLARVSEEAGYSHIWVGDSHLIWREAY
                     VNMAAMALNTTKVKLGTGVTNPLTRHPSVVGSAYATLEEYAPGRMIVGIGLGDSSVET
                     MGMKPAKLSYFETTIHQMRDLLDGKEVQLETGKIHVLHPCQRKVPIYIAASGPKMLEL
                     CGRIADGVIVLVGVADEYIEHAKEKIAAGAKLVGRKLTDIQLVLWVPCAVSENTSAKD
                     AVKAHVARVVAHPLPYVLDPNEQKVLAEIRKTYDYYHHMDQQANHAEVIPDWLVDKFA
                     IAGTVDECRGQIERIKKTGIQQVAIIPYSPPGGSREETIRGFARAMN"
     gene            complement(4681..5853)
                     /locus_tag="VFY96_01385"
     CDS             complement(4681..5853)
                     /locus_tag="VFY96_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020497946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5605134.1"
                     /translation="MPSILIKNIGAFVTGKLENPLREADSIFIKSGVVEAIGNGLSEP
                     ADQTIDANGITAIPGLIDSHSHPSIGEYTPAQNALGWITNYMHGGVTSLISAGELHLP
                     GLPLPPDARTALSVAIVTKKCYDNLRPSGVKVHAGTLLLVPGLSERDFDEIHSLGIKL
                     VKFIFYDYSLLPNGEAERYVNWAHARGIKVKIHSGGVSRSGVSQIADFAMVQKIKPDI
                     IGHITGGPIPMPEEDMIRIASETECFMEICTSGNYRLALVLARLVKTKNLHHRVLIGT
                     DTPGGTGIVPRGILREVAFLSSVGEIEPEKAICMATGNVGRAHGLDIGILEEGRPADL
                     VLLHKITGSVAKDALDSFGKGDLPGISTVIIDGEIRVTGRSQQTPPPERVAALKDK"
     gene            complement(5926..8613)
                     /locus_tag="VFY96_01390"
     CDS             complement(5926..8613)
                     /locus_tag="VFY96_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5605135.1"
                     /translation="MKLRKWIKYRLLTVTLGWLCLVISGPLAARAATQTEITRATLKN
                     GLRVVIVRNALAPVVTTEINYLVGSNEAPEGFPGMAHALEHMMFRGSPGLSAAQLSSL
                     IAAMGGSFNAATQQVVTQYFFTVPATDLDTALQIEAIRMRDVLATDALWKQERGAIEQ
                     EVAQDYSNPEYLFYSRLLAQMFSGTPYAHDALGTRPSFQKTTAAMLRNFHRKWYAPNN
                     AILVIVGDVNPDSALATVRQLFESIPRRSVPPRPKIALRELKPAKIEIDSDLPYGLAA
                     VAYRMPGYSDADFAASVVMADALDSRRGELYGLAAEGKALASGFDASALPAAGFGYAH
                     AAFPQGQDSAALVSEMKQVIARYLDHGFSADLVEATKRREVAEAEFRRNSISGLAAEW
                     SQALAVEGRNSIDDDIEAIKKVTVADVNRVAKKYLTNDKAIVVIMTPHASGEAVASQG
                     SRSGGESFAPKQAKAVPLPAWAKRAVSPLSVPVSTVQPTDITLANGLRVIVQPESISD
                     TISVFGRVKNRPELQVPPGKEGTDQILRDLFSYGSVELDRLAFQEALDQIGGQLHAGT
                     SFSLEVLPDAFGRAVELLADNLLRPALPEAAFKAVQQRTRQAVAGQLQSPIYRSGRAF
                     LKAIYPEKDPTLREATPETVASLTLNDVRQYHEQVFRPDLTTIVVIGKVTPEQARAVI
                     EKYFGSWKAAGPKPETDLPPVPRNKASFIVVPDRSRVQDEVNLGQTFELKRQDEDYYP
                     LQAGIQILSGGFYASRLYQDLRETSGLVYSIGAELQSSKTRSVFAVSYACDPPNVAKA
                     RALVERNLRLMQTKPVTAGELEQAKILLLRSIPLSESSVHGIASGLLDRSLRDLPLDE
                     PITAAKRYVNLTAAEIQAAFSKWLKIEDLAQVVLGPAPK"
     gene            complement(8622..9128)
                     /locus_tag="VFY96_01395"
     CDS             complement(8622..9128)
                     /locus_tag="VFY96_01395"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX5605136.1"
                     /translation="MLEKFLLWRHQHVLGSNHGVHPVGYMNLLADGAHNFIDGVIIGA
                     SYAINLHVGLATTLAVVFHEIPHELGNFFILLYAGFSKGKALFFNFLSALFAVLGTII
                     SLLIGSSIADFSGVMLPLAAGGFIYIAGSDLVPELNKESTLGKSLVQLLAIGLGVGLM
                     LLLTLLES"
     gene            complement(9108..9353)
                     /locus_tag="VFY96_01400"
                     /pseudo
     CDS             complement(9108..9353)
                     /locus_tag="VFY96_01400"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
     gene            complement(9384..10832)
                     /locus_tag="VFY96_01405"
     CDS             complement(9384..10832)
                     /locus_tag="VFY96_01405"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX5605137.1"
                     /translation="MNGFFTMLNPSYLLFPALVGTLIMGFVCPLIGSYLVLRRTIFLG
                     LTLPEIAAAGVSFTFWLQQTGILPEFAQGERSIGMIGSLLFTFLGMALLGYLEQRGKG
                     MAEGRLAAAYAFAGAVTILFMVFNPAGQVDVLNLLKGEVIALSRGELKLLAVVFGLVA
                     MLMLVFRREFLLISFDRDLAFLLKGRQIIWDILLYLLAGIVIALGVIMAGPLLIFGFL
                     VLPALAAKPLVSRMSGFLLLSSVLGLVMAFVGFYSSVRLDLPLGPTDVALGCCLIFLA
                     YGLSRISVRRRVALMLMALVVVSASACGAAKTMSPIPEARALNPQPLWLAKVKNSTAL
                     PLLLPSSNPLRSLAEMAGKVSSDYRSSVMDLLREDLRTELEQRGFKVALPEQVDVRFT
                     AFTSDPDSALRLAREGKLSGALFVSEIWRWEGETQKFVRALVDFKLIQIDDGTVVWQR
                     RVQGAIPTPSATNLGQASADAVRAIVRELFPG"
     gene            complement(10832..11548)
                     /locus_tag="VFY96_01410"
     CDS             complement(10832..11548)
                     /locus_tag="VFY96_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEX5605138.1"
                     /translation="MKTANTEASIVGNPATAAAVAPLIVMENTSMGYNSGAILTDVNL
                     AIYPGNLVGLAGPNGSGKTTLFRTVLGLLAPLSGRLVRHCPLAKFGYVPQSAALDPQF
                     PLSVLEIVEMGAYGRVKSFQIFPAPETKRALNVLEQVGLAHVKSKSFFALSGGQKQRV
                     LIARALMVDPAIMILDEPLSGVDEESRQSITALLIRLSREERLAVFFSSHDLEMVQRV
                     ADKIVRVDRGAVWVEERSGS"
     gene            complement(11552..12475)
                     /locus_tag="VFY96_01415"
     CDS             complement(11552..12475)
                     /locus_tag="VFY96_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEX5605139.1"
                     /translation="MKTLLKIAKWQALTILLAAAGISRPAVAQPIRVVATFPDLADIT
                     RQIGKNLVSVESLATGVEDPHGVPMKPSFVPRLNRANVLVVTGLDDEHSWLPALLEVA
                     SNPKILSGQSGYIDCSVGIPVLEAPTRLDRAEGDLHPKGNPHYLLDPTYGKIAAQNIA
                     AGLARSFPQYQADFDKNLKAYLGELDNWISRWEKIAAPLRGVKYVEYHPEWVYFANRF
                     GLKRVGSIELKPGIEPTPNHLVALVQQIKQDKAQVLIYGSQNPRIPQQLQGETGIKVL
                     RLYTSAGARSETDTYIKWIDYTVHSLVQAVS"
     gene            complement(12494..>13448)
                     /locus_tag="VFY96_01420"
     CDS             complement(12494..>13448)
                     /locus_tag="VFY96_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605140.1"
                     /translation="QRSKAGGNFNFRSAEIGLAASVDPFARMYGFINGTSDGVEVDEA
                     AAVTTSLPWNLTAKGGRFLADFGRFPKIHEHELPFVNQPLSIERMIGGETRADGAELN
                     YLFPTPFFLRATLGGYNEIGEENQRVDNRKSRSWSRFTYLGRLNTYFDLSDNHSIELG
                     TSMAYTPSVRLTGAADGGDRMLNGIDLTYRFQPLASTVYQGVTVGSEIFRNSEQFAFE
                     SAAKRRESFGGYSYAELKLSKNWSTGFLFDYAPSVSSPGKKTIGYSPFVTWNMSEFNR
                     LRFQFTHADDRVREDKNDGGNQFFLQWTTVIGAHTHGFLGR"
BASE COUNT         3008 a         3908 c         3630 g         2902 t
ORIGIN      
        1 tcgagaaagc acatcccgat gcaaagctcg atcttgacta caaaaatcct ctggaactgc
       61 ttatcgcatt gattctcgcc gcacaggcgc gagacgacct ggtaaatcaa atcacggcca
      121 atctcttcaa gatccagcgc agcgccgccg attacgcgaa cacaccgctg acgagcctcg
      181 aaaggcagat cggcaaaata aatttctacc gcaccaaagc cagatcgatt cacaactgct
      241 gtcaccacct ggttgatcgt ttcgatggca aggttcccgc caatttggat gacttgctct
      301 cgttgcccgg cgtcggacga aaaaccgcga acattttgct gggcaatgct tacggccgac
      361 agaccatcgg cgtcgatact cacgtcttgc gactgtcgca gcggctcgga tttacgaaaa
      421 acaccgaccc ggacaaaatc gaagccgatc tcatggtgat cgtccccgac aagcagcgcg
      481 tgcgtttttg ccatctgctg cagtatcacg gccgacgcct gtgctgggca aaaaaaccga
      541 attgtcccgc ctgcacgatc cgtgagctct gcccatttcc acacaaaacc aaatccgttg
      601 tcggtgcgat ccggcattcg ccgcgccatc ctagtaaatc caaacaacaa accgggtaaa
      661 cacaggcaac cgattctgtt gagccctgct ttgccttctc gatgcggtaa gtcatgacat
      721 tgtgtattgc tcgtgccccc tgtacactga gaacgtcact cgacgacatc gatccatgcc
      781 cgactcgttt gaagctcccg acccttctcg tcttttcctt gaagctcgcg gtttaatcga
      841 attgccactc ctattgtttc gctttccaga gcttgcctgg cagccccgcg gagaaggccg
      901 accggttttg attttgcccg gctacggcgc cggtgatggc tcgactgcga tattgaagag
      961 ctatctcaaa ttgctagggt atcgagcccg tggctggggc ctcggacgga atactggcac
     1021 cgccgcggat ttgctgccga gaatcttgaa gcgggtctcc tccttggctc gccgcaccca
     1081 acaacaagtt catctcatcg gctggagttt cggcggttat ctcgcccgcg aactggcgcg
     1141 tgagcgcgcc gatctgatcg gccaggtcat tactttgggc acgcccgtca tcggcgggcc
     1201 caagtatacg gtcgtcgcca gatcctttca tcgccgcggc attgatatgg atgcgatcgc
     1261 tgccgaggtc gaaatgcgca atcggatcac cttcactacc ccggtgatcg ccctctattc
     1321 gcgtcgcgat cgggtggtcg cttggcaggc ttgcattgat gccaacgcgc aaaatgtcga
     1381 gcacgtcgaa gtacgcacca ctcacttcgg tttcggcttt tcaccggaag tttacaaaat
     1441 catcgcccag cgattggcgg caaatcgcgc atcggcaacc ggacgagcgc aacgtgagtt
     1501 gtggcgctgg cgctggccgg gtgcgagccg gcaagccaaa cgctgatcgg gtcagccccg
     1561 aatccgatcc tccgccatgg acagcgcttt cttcctgtgg ccgattgcgg gagttctcgt
     1621 gttagccgga ttggcggggc taatcctgcc ggcaatcccg ggtgcgccgt tgattttcgc
     1681 aggattagtg gttggcgcct tggccgatga cttcgtctac gtcggagcgt ggacccttat
     1741 cgcgctcgcg gttctgaccc tgctcataat cggtgttgat ttctgggcga ctatgtttgg
     1801 cgcaaaaaag tttggcgctt cccgacgcgc tgtgattgga gccgttgtcg gttccattgc
     1861 cggcctattt ttaggcgtcc ctggcgtgct ctttgggccg tttattggcg cggtgctcgg
     1921 cgagatctca tcggccggca gcttgcggca ggcgacgcgc gcaggtttcg gcgcgactct
     1981 tggcctgatt cttggtgctg caatgaagct ggccttggcc ttctccatga ttggcatctt
     2041 cgtggcggta cgctttatac agtgattgat ctgcgaatgg agtagaaagt ctttatggaa
     2101 tcaccaaacc ggatacaccg gacacgggcg tttcaccgag gagtgcaaat gaaaacactt
     2161 acggttctct tcgcggcttt aatcattcat ggtcaggcgc tcgctctcac catcgccacc
     2221 ggctcagccg aagggactta ttttaagatc gcccaggaca tcaagcagat cgccgagaaa
     2281 gaaggaattc ccatcgaagt cgttcagacc aatggttcct tcgataatat taacctcctc
     2341 ggcgcgggca aagtggatct cgcaattttg cagctcgacg tgttgcgttt cgtcgctgag
     2401 atcatgctca aggagaccgg cttcaacgtc ttgcaggaag cgaaagtggt tctcaatttg
     2461 tacccggaag agatccatat catcgctaag gatgaaaaaa tcagaaccgt ccaagagctc
     2521 caaggcaaac gagtggccgt gggaccggag aagagcggtt cggcgctgac cgcggaagtt
     2581 ttgctcgctg gattcaatgt gcacatcgac cgcgcgtttg ataccccaga agaggcgata
     2641 aagaaaatcg tcagcggcga tctcgacgcg ctcgtgttcg tgggcggcgc gcccgttccg
     2701 gcgtttcgca atctcgatcc gagttttcat ttcgttacga ttcccgcgga gagcggcttg
     2761 gagcagattt acccgaaaaa aaagatcgag cagaacgtgt atagttgggc tggcgaagtg
     2821 gagacccacg ccgtgccgtc ggtcattctc acgagaaacc gcgacgatcg cgactttgtt
     2881 gtcagcttgc agaagctcgt gctctcgatt ttagtcaata aagagagctt ggacgcgaac
     2941 ggtcatccga aatggaagga ttcctatgtg cgctcgaccc ttggcggcgt cggttatgga
     3001 ccgaccacag aggtgatttt gttgtacaat actctcgata gcgccggcta ccgtatcatc
     3061 aaaaaatagc tgcccgttgg tttactcgat caatcgggac gggttgtcag ttcgatcttg
     3121 tacccgttgg gatcttcgag aaatgcgatg gcgttgctcc cgctgccgtg cttcattggc
     3181 cccggttcac ggacaatctt aatccctttg gcgcgaaact cttcgcaggc tttgtaaacg
     3241 tccggcacgc cgagtgcgat atgtccgaac ccgttgccga gatcgtatgt gtgcgtatcc
     3301 cagttgtagg tgagctcgat cacggcgtca gtttcttccg acccatagcc gacgaaaaca
     3361 ttggtaaatc ggccggactc gtaatctctt ctctttagca acttcatgcc gagcccatta
     3421 cagtagaact tcaaactctc gtcgagatca ttcacccgaa tcatcgtatg gacaattttc
     3481 atgtgaatct cctctccaga ttggccctga tcactgccgc gcaatgaatg gccgtcgtac
     3541 cggcgaaagc cggtacccaa gttcgcaatg ctgactcagt ggcccagcaa gaactattcg
     3601 cgtttaccga accatttggc aatcgccctc agttcatcgc tctggcgaat ccccgaatgg
     3661 tttcttccct gctgccgcct ggcgggctgt agggaataat cgcgacttgc tgaatgcccg
     3721 tcttcttgat tctttcgatt tgcccgcgac attcatcgac cgttccagcg atggcgaatt
     3781 tgtcgactaa ccaatcggga atcacctcag catggttcgc ttgctgatcc atgtgatggt
     3841 aatagtcata ggtttttcgg atttccgcca aaaccttctg ttcgttggga tcgaggacgt
     3901 agggaagcgg atgcgcaacc acacgagcga catgcgcttt gacggcgtcc ttcgcggagg
     3961 tgttttccga aaccgcgcaa ggcacccaaa gcaccaattg aatgtccgtt aatttgcgcc
     4021 ctacgagctt ggcgccggca gcaatctttt ctttggcgtg ctcgatgtac tcatcggcga
     4081 cacccaccag cacaatcacg ccgtcggcaa tccggccgca gagctcgagc attttcggtc
     4141 cgctggcggc gatgtaaatc ggcacctttc tttggcatgg gtgaagcacg tggatcttgc
     4201 cggtttcgag ctgcacttcc ttaccgtcga ggagatcccg catttgatgg atagtggtct
     4261 caaagtacga tagcttggcg ggtttcatgc ccatggtttc caccgagctg tcgcctaaac
     4321 cgatcccgac gatcatccgt ccgggcgcgt actcctccaa cgttgcgtag gcgcttccga
     4381 ccaccgaggg atggcgcgtg agcggattgg tcacgcccgt gccgagctta accttggtgg
     4441 tattgagcgc catcgctgcc atgttgacgt aggcctcgcg ccagatcaga tgcgagtcgc
     4501 cgacccaaat gtgactgtag cccgcttcct cgctcaccct ggccaggtga atcatcttct
     4561 gtaacggctc agtcggaaat aatccaactc cgtgatccat tgttcactcc gggagcgagg
     4621 accgagaatg gcgggtcgag gatcgcttct cgatcgatcc tccacatcta tcctcaatcc
     4681 tcatttgtct ttcagcgccg cgactcgctc gggcggcgga gtttgctgac tccgccctgt
     4741 gacccgaatt tcgccatcga taatcaccgt cgatataccc ggcagatcac ctttgccgaa
     4801 cgaatcgaga gcgtccttgg caaccgaccc ggtgattttg tgcagtagca cgagatccgc
     4861 cgggcgtccc tcctccagta tcccgatatc aaggccgtgc gcccggccga cgtttccagt
     4921 cgccatgcaa atagcttttt ccggttcgat ctcgccgacc gaagataaaa aggccacttc
     4981 gcgcaaaatg ccgcggggaa caatcccggt tcctccgggc gtatccgtcc cgatgagcac
     5041 ccgatggtgc aaatttttcg ttttgacgag ccgcgcgagc accagtgcca ggcgataatt
     5101 gcccgacgtg cagatctcca tgaaacattc ggtttcgctg gcgatgcgga tcatgtcttc
     5161 ctcgggcatc gggatcggcc cgccggtaat atgaccgata atgtcgggct ttattttttg
     5221 caccattgca aagtcggcga tttggctgac accggagcgc gacacaccgc ccgaatgaat
     5281 ttttaccttg atgccgcgcg catgcgccca gttgacgtag cgctccgctt caccgttagg
     5341 caaaaggctg taatcgtaaa aaataaattt gaccagcttg atgccgagcg agtggatttc
     5401 gtcaaaatcc ctttcactca gtcccggaac cagcaataag gtcccggcgt gcactttcac
     5461 acctgaaggg cgcaggttgt cgtagcattt cttggtgacg atcgcgacgg acagcgccgt
     5521 gcgcgcgtcc ggcggcagcg gcagaccggg cagatgcagc tcgccggcgg agatcaatga
     5581 cgtcacgccg ccgtgcatgt agttggtgat ccacccgagt gcattctgcg ccggcgtgta
     5641 ctcgccgatg gacggatgcg agtgagaatc gatcagaccg ggaattgccg tgatcccgtt
     5701 cgcatcaatc gtctggtccg ccggttcgct caagccattg ccgatcgcct caacaacacc
     5761 gctcttgatg aaaatggaat ccgcttccct gagcggattc tccaattttc cggtgacgaa
     5821 agcaccgata tttttgatca ggatcgaagg catgcgcgga ctataccaaa ggttttacaa
     5881 cagcaaaagg aaacgttctt attggcaaaa attctgccgc gggctttact tgggagcggg
     5941 ccccaacacg acctgtgcga ggtcttcaat cttgagccat ttcgaaaaag ccgcttgaat
     6001 ctccgcagcg gtcaaattga cgtaacgctt cgctgcggtg atcggctcgt cgagtgggag
     6061 atcacgcaac gaacggtcaa gcagcccgct cgcaatacca tgaacgctcg attcactcag
     6121 aggaatgctg cgcagcaaca gaatcttcgc ctgttctaac tcgccagccg tgaccggctt
     6181 ggtttgcatt aaacgcaggt ttcgctccac gagcgcgcgc gctttggcga cattgggcgg
     6241 atcgcaggca tatgagacag cgaacaccga acgggtcttg ctcgattgca gctcggcacc
     6301 gatggagtaa accaaaccgc tcgtttcgcg caaatcctga tagagtcggc tcgcatagaa
     6361 accgccggac aaaatttgta ttcccgcctg cagcggatag taatcctcat cctggcgttt
     6421 gagctcgaaa gtttggccga ggttaacctc gtcttggact cggctccggt ccgggacaac
     6481 gataaaactc gccttgttgc gcggcaccgg cggcagatcg gtttcaggct ttggcccggc
     6541 cgctttccaa gagccgaaat acttttcaat cacagcacgc gcctgctcgg gtgtcacctt
     6601 tccgatcaca acgatcgtgg tcagatcggg acgaaaaact tgctcgtgat actggcgcac
     6661 atcgttcaag gtcagcgaag cgacggtctc gggcgtggct tcgcgcaagg tgggatcttt
     6721 ttccggatag atcgctttga gaaaggcgcg acctgaacgg tagatcgggc tctgcagctg
     6781 gccggcgaca gcttggcgtg tgcgctgctg cacggccttg aacgccgcct caggcagcgc
     6841 cggacgaagc aagttgtcag ccaacagctc gacggcgcgc ccgaaagcgt caggcagcac
     6901 ttcgagggaa aagctcgtgc ccgcatgtaa ttggccacca atttggtcga gcgcctcctg
     6961 aaatgcgaga cggtcgagct cgaccgatcc atatgaaaac agatctctca ggatctgatc
     7021 tgtgccttcc ttgcctggcg gtacttggag ctcggggcga ttcttgaccc gcccgaagac
     7081 gctaatcgta tcgctgatcg actccggctg tacgatcacg cgtaaaccgt tcgccaaggt
     7141 aatatcggtc ggctgcaccg tcgagaccgg caccgaaagg ggcgaaaccg ctcttttcgc
     7201 ccaggcgggc aatgggacgg ccttcgcttg ctttggcgca aatgactcgc cgcccgaacg
     7261 cgagccttgc gatgcgaccg cctctccgga cgcatgcggc gtcatgatca ccacaatggc
     7321 cttgtcattg gtcagatatt ttttcgccac ccggttcacg tctgccacag tgactttctt
     7381 gatcgcctcg atatcatcgt cgatagaatt cctgccctcg accgcgaggg cttgcgacca
     7441 ttcagcggct aaaccggaaa tcgaattgcg gcgaaactcc gcttcggcca cctcacgccg
     7501 tttggtggct tcgactaagt ctgcactgaa accgtgatcg agatatcgcg caatgacttg
     7561 tttcatctcg gaaaccagcg cggcactatc ctgcccctgc ggaaacgcag cgtgggcata
     7621 gccaaatccc gccgccggca acgcgctcgc atcgaaaccg ctggccagag ccttgccttc
     7681 ggctgctaat ccgtaaagtt caccgcgccg gctatcgagc gcgtcggcca taactacgct
     7741 cgccgcgaaa tcggcatcgc tgtaaccggg catgcgatat gccaccgcgg ctaatccata
     7801 gggcaggtcc gaatcaattt cgattttggc tggctttagc tccctcagcg caatcttcgg
     7861 tcgaggcggc acgctccggc gcggaatgga ttcgaacagc tgccgaacag ttgccaaggc
     7921 tgagtcggga ttcacatcac cgacgatgac gagaatcgca ttgtttggcg cataccactt
     7981 tcgatgaaaa tttctgagca tcgcggcggt ggttttttga aatgaaggcc gcgtaccgag
     8041 cgcatcgtgc gcatacgggg tgccggagaa catctgcgcc agcaggcgcg aataaaataa
     8101 gtattcggga tttgaatagt cctgcgcgac ctcctgctcg atcgcgccgc gctcctgctt
     8161 ccatagcgcg tcggtggcga gcacatcgcg catcctgatc gcctctattt ggagcgccgt
     8221 gtccagatca gtcgccggga cggtaaagaa atactgggtg accacctgct gcgtggccgc
     8281 gttgaaactt ccgcccatag ccgcgatcaa actcgacaac tgagccgccg atagtcccgg
     8341 gctgccgcga aacatcatgt gctcaagggc atgcgccatc ccgggaaaac cctccggcgc
     8401 ctcattggac ccgaccagat agttaatttc tgtcgtcacc acgggcgcca aggcattacg
     8461 gacaatcact acacgtagcc catttttgag cgttgcgcgt gtgatctcgg tttgtgtggc
     8521 cgcccgcgca gcgagtggcc cgctgatcac caggcataac catccgagcg tcaccgtgag
     8581 aagcctatat ttgatccatt tacgaagctt cataatcagt cctacgattc caacagagtt
     8641 agcagaagca tcaatccgac tcccaatcct atggcgagca gttgtaccag cgatttgccc
     8701 aacgtcgact ccttgtttag ctcaggcacc aaatcggatc ccgcaatata gatgaatcca
     8761 ccggccgcca ggggcagcat cactcccgag aaatcagcga tgctggaacc gatcaagagc
     8821 gagatgatgg tccccaaaac cgcaaaaagc gccgacaaaa aattgaaaaa taatgccttg
     8881 cccttgctga agccggcata gagcaaaata aaaaagttac ccagctcatg cgggatctcg
     8941 tgaaaaacca ccgccagggt cgttgccagc ccgacgtgga gattgatggc gtaagacgcg
     9001 ccgatgatga cgccgtcgat aaaattgtgc gcaccgtcgg cgagcaggtt catatacccc
     9061 accggatgca ccccgtgatt cgagccgagc acgtgctgat gccgccagag gagaaacttc
     9121 tcaagcacaa aaaacgcgaa aattccggca agcaccaaga ttgaagcttg ttctttgctg
     9181 cccagccgtt cgaaggactc tggcaagaga tgaatgaaag catcgccaaa caacccgccc
     9241 accgccaagc tcaccataaa aaagataatg cgcttgagcc ggtcttcgcc gatggagata
     9301 aacgcgagcc cgatcagcga cagcagactg actacgacga cgctggcgag actatataac
     9361 caagtcgctg acgtggaatc ctcttaacca gggaatagct cgcgcacgat cgccctgacg
     9421 gcatcggccg acgcttgtcc gagattggtc gcactcggcg tgggaatggc tccttgaacc
     9481 cgcctctgcc acaccaccgt tccatcatcg atctgaatca gcttaaagtc caccaaggcg
     9541 cggacgaact tctgcgtctc tccttcccaa cgccaaatct cgctgacaaa cagcgcccct
     9601 gaaagctttc cttcgcgggc taatcgcagc gcgctgtcgg gatcagacgt gaaagcagta
     9661 aagcgcacat cgacttgttc cggcaaagcg accttgaatc cccgttgttc gagctccgtt
     9721 cgtaaatctt cccgaagcag atccatgaca ctcgatcggt aatccgaaga gacttttccg
     9781 gccatctcgg cgagggaacg aagcggattg gaagacggca acaaaagcgg cagcgctgtg
     9841 ctgtttttca ccttggccaa ccagagcggt tgtggattga gagctctggc ttcagggatt
     9901 ggcgacatcg ttttcgcggc gccacaagcg ctggctgaaa caacgactag cgccattagc
     9961 atgagcgcca cgcggcgtcg cacagagatc cggctcaatc cgtaggcgag aaaaatcaag
    10021 caacatccga gcgccacgtc cgtcgggccg agcggcagat caagccgcac cgagctgtaa
    10081 aagccgacaa acgccatcac caatccgagc acggaggaga gcagaagaaa accgctcatg
    10141 cggctgacca aaggtttggc cgccaacgcc ggcaacacga gaaagccaaa aatcaagagc
    10201 ggcccggcca taatgacacc gagagcaata acgatcccgg caagcaaata aagcaggata
    10261 tcccaaatga tctgtcgtcc tttcaggaga aatgccaaat cgcgatcgaa agaaatcaac
    10321 agaaactctc tgcgaaaaac aagcatgagc atggcgacaa ggccaaagac caccgccaac
    10381 aatttgagct cgcctctgga cagggcgatc acttcgcctt tgagcaggtt cagaacgtcg
    10441 acctgaccgg cggggttgaa aaccataaac aagatcgtta ccgctccggc aaacgcgtag
    10501 gccgccgcga gacggccttc cgccatgccc ttgccgcgct gttctagata cccgagcaaa
    10561 gccatcccga gaaacgtaaa tagcaacgag ccgatcatac cgatgcttcg ttctccctgt
    10621 gcaaactcag gcagaattcc agtttgctgc aaccagaacg tgaaagaaac ccccgctgcg
    10681 gcaatttccg gcagagttaa gccaagaaaa atcgtcctgc gcaaaaccag gtaagaaccg
    10741 ataagcggac agacgaatcc catgatcagc gtgccgacga gcgcggggaa gagcagatag
    10801 gacggattga gcatcgtaaa aaagccattc atcaagagcc gctcctttcc tcgacccaaa
    10861 ccgcgccccg gtcgactcgc acaatcttgt ctgcgaccct ttgcaccatt tccaaatcgt
    10921 ggctcgaaaa gaaaaccgcc agccgctctt cacggcttaa tcggatcagt agagcggtga
    10981 tgctctgccg cgattcctca tcgacaccgg agaggggttc atcgagaatc atgattgcgg
    11041 gatcgaccat taaggctcga gcgatcagca cgcgctgttt ttgcccaccg gaaagagcaa
    11101 aaaaagattt gcttttaacg tgcgccaggc cgacttgctc gagcacgtta agagcccgct
    11161 tcgtttctgg cgcggggaag atctgaaatg atttcacgcg gccataggcg cccatctcga
    11221 caatctcgag aacgctcaac gggaactgcg ggtcgagggc agcgctttgc ggaacatagc
    11281 cgaacttcgc caaggggcaa tggcgtacga gcctgccgct gagcggagcc aataacccga
    11341 gcaccgttct gaatagtgtc gtcttgccgg atccattggg gcccgcgagt cccacgagat
    11401 tgcccgggta aattgccaga ttgacgtccg tcaaaatggc cccggaattg taacccatgc
    11461 tggtattttc catgacaatc agaggggcga cggcagccgc cgttgccggg tttccaacga
    11521 ttgaggcctc ggtattcgct gtcttcatcc tctaactcac cgcctgcact aagctatgca
    11581 ccgtgtaatc gatccatttg atataggtat cggtttcgga ccgagcaccg gcgctggtgt
    11641 agagtcgcag gactttgatg ccggtctctc cttgaagctg ctgcgggatg cgcgggttct
    11701 gcgagccgta aatcagcact tgggccttgt cctgcttgat ctgctggacg agcgccacga
    11761 gatgattggg cgtgggctcg atgcccggct tgagctcgat gctaccgacc cgcttcagac
    11821 cgaagcggtt ggcgaaataa acccactccg gatggtattc cacgtatttt acaccgcgga
    11881 gcggcgccgc gatcttttcc cagcgactga tccagttatc gagctcgccg agatacgctt
    11941 tcaaattctt gtcgaaatcc gcttgatact ggggaaagct tcgcgccaat ccggcagcaa
    12001 tgttctgagc ggcgattttc ccgtacgtag ggtccaaaag ataatgtgga tttcccttcg
    12061 gatgcaggtc cccctcggcg cgatcaagcc gcgtgggagc ttccaaaact ggaatgccga
    12121 ccgaacaatc aatgtagccg gattggccgg aaaggatttt cgggttgctc gccacctcga
    12181 gcagcgccgg cagccaggaa tgctcgtcat cgagcccggt caccacaaga acgttagcgc
    12241 ggttgaggcg cggcacgaaa ctaggtttca tcggcactcc gtgcggatct tccacgccgg
    12301 tcgccaggct ttccacactg accaaattct tgccgatctg ccgagtaata tcggcgagat
    12361 ccggaaacgt cgcgacaact cgtatgggct gcgctacggc cggtcgggaa attccggccg
    12421 cggccagaag aattgttaag gcctgccatt ttgcgatctt gagcaaagtt ttcattcgcg
    12481 ggtcctttca gtgctagcgg ccaagaaagc cgtgcgtatg ggcgccgatg accgtggtcc
    12541 actgtagaaa aaactgattg ccgccatcat ttttatcttc gcggacacga tcatccgcat
    12601 gcgtgaattg gaaacgcaac cgattaaact cgctcatgtt ccaagtaaca aacggcgagt
    12661 agccgatggt cttcttgcct ggacttgaga cactcggtgc gtagtcgaaa agaaatcccg
    12721 tggaccagtt cttactaagt ttcagttcgg catagctgta gccaccgaag gattcccggc
    12781 gtttcgcagc actttcaaag gcgaactgtt cactattgcg aaaaatttcc gagccgacgg
    12841 tcactccctg gtaaaccgtg ctcgcgagcg gttgaaaccg ataggtcaga tcgatgccat
    12901 tgagcattcg atctccccca tcggcagcgc cggtgagacg gaccgacggc gtgtacgcca
    12961 tgctcgtacc cagctcgatg ctgtgattat ccgatagatc aaagtaggta ttgagcctgc
    13021 cgaggtaggt gaaccggctc catgagcgcg atttgcgatt gtcgacccgc tggttttctt
    13081 cgccgatctc gttatagcca cctaacgtgg cgcgtaaaaa aaacggcgtc ggaaaaagat
    13141 aattgagctc cgcgccgtcc gcccgggttt cgccgccgat catccgctcg atcgaaagtg
    13201 gttggttcac gaacggcagc tcgtgttcat gaattttcgg aaatcgtcca aaatcagcca
    13261 ggaagcgccc acctttggcc gtcagattcc agggtagtga ggtggtcacc gccgctgcct
    13321 catcgacttc aacgccatcg gaagttccat tgatgaagcc atacatcctg gcgaacggat
    13381 cgacggaagc agccagcccg atttcggcgc tgcgaaaatt gaaattacct cccgccttgg
    13441 agcgctgg
//
LOCUS       DASULZ010000068         2960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_920518, whole genome shotgun sequence.
ACCESSION   DASULZ010000068 DASULZ010000000
VERSION     DASULZ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920518"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1590
                     /gene="gspE"
                     /locus_tag="VFY96_01425"
     CDS             <1..1590
                     /gene="gspE"
                     /locus_tag="VFY96_01425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HEX5605141.1"
                     /translation="TRLVVELGFLSEEDLLPVLRDHFELPLISLKDFPPTALPIDLPP
                     GIGDFFKLARMVPVKIEGRELLVATTDPFDVSHLHALELAVGLNVRPVLAREKEIAAR
                     IDALYSSGYAGDGRQSAAEATDAIGEDEDVAHLRDMASEVPVIRMVNQMLVRALESRA
                     SDVHIEPFENQLKVRYRIDGILHEVESPPRQLKGAVISRLKILAQLNIAERRLPQDGR
                     IKIRLAGKDVDLRIATVPTLYGESVVIRLLERGQIFTDLDTLGFPPALLAKFSEMISK
                     PHGMLLVTGPTGSGKTTTLYGALQKINDPGKKIITIEDPVEYQLSGVNQIYVKPQIGL
                     TFANGLRSIVRQDPDVIMVGEIRDSETAEIAVQAALTGHLVLSTLHTNDAAGAISRLL
                     EMGVQDYLLSSSLLGVLAQRLVRRLCPQCRREVPFAGLEGAEAELDLLNGNRIETVWE
                     AVGCDGCNATGYLGRIGIFELLPVTSEICKIIVQRADAGAIRNLAVRQGMRLLREDGW
                     DKVRRGITTLSELLRVTREEA"
     gene            1721..2944
                     /locus_tag="VFY96_01430"
     CDS             1721..2944
                     /locus_tag="VFY96_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX5605142.1"
                     /translation="MAFFQYRAADQAGKLVEGVMEADAEQSVVARLREMGCVPLRIAL
                     PTGRPATTIRNPLPLGSRRKVSQQQLLQFTRELATLLGAGLPLDRSLSILGGLIEGGE
                     FSKVLRSLVEAVRAGKSLAASMGEYPDVFPKIYVNMIRAGEAGGILEGVLRYLTDYLE
                     STVALKEDIKSALIYPIILASAASLSLVVLFVFVIPKFATIFRDNGKALPVITKIIIE
                     LSQILAQYGWVILLLLCAAIVGALFYIRSPEGRGEWDRMCLRSWLLGDLVRKFETARF
                     CRTLAALLKGGVPVLEALGTVQGVIGNRLLARAITQVQARVREGKGMARPLNESGVFP
                     SLALNMITVGEETGKLDNMLAEVANYYDQEVKRATKRLTSLLEPVLILGMGLIIGIVV
                     ISMLMAIFSINDLPF"
BASE COUNT          633 a          763 c          870 g          694 t
ORIGIN      
        1 acgcgcctgg ttgtcgaatt ggggtttctc tccgaggaag atctgttgcc ggtcttacgc
       61 gaccactttg agctcccttt aatttctctc aaggattttc cgccgacggc gctgcccatc
      121 gaccttcccc cggggattgg agattttttc aagctggcgc gcatggttcc ggtcaaaatc
      181 gaagggcgcg agctcctcgt tgccaccact gatccgttcg atgtgtctca tttgcatgcg
      241 cttgagcttg cggtgggatt gaacgtgcgg ccggtattgg ctcgtgagaa ggaaatagcc
      301 gcgcgcatcg atgcgcttta cagtagtggc tatgctggcg atggtcgtca gagcgcggcc
      361 gaggcgactg acgctatcgg tgaggacgaa gacgtcgcac acctgcggga catggcatcc
      421 gaggtacccg tgattcggat ggtgaaccaa atgctggtcc gggcgttgga aagccgcgcc
      481 tccgacgtac atatcgaacc cttcgagaat caactaaaag ttcgttaccg gatcgatggc
      541 attctccatg aagtcgagtc gccgccgcga caactgaaag gcgcggtcat ttcgcgtctg
      601 aaaattctcg ctcagctcaa cattgctgag cgtagattgc cgcaggacgg ccgaatcaag
      661 attcgtttag ccggcaaaga cgtcgatctg cggatcgcca ccgtgccgac cctgtacgga
      721 gaaagcgtcg tcatccggct tttggaacga gggcagatct ttactgatct cgatactctg
      781 gggtttcctc ccgccttgct ggcaaagttc agcgagatga tctcaaaacc tcacgggatg
      841 cttttggtga ctggccctac cgggagcggt aaaacgacaa ccctgtatgg tgcgctgcaa
      901 aagatcaacg atccgggcaa aaagatcatt accatcgaag acccggtgga atatcaattg
      961 agcggcgtca accagatcta tgtcaagccg caaatcggtt taacctttgc caacgggcta
     1021 cgctcgatcg tccggcagga tccggatgtc attatggtgg gggaaatccg tgactcagaa
     1081 accgccgaga ttgccgtaca agctgcgctg acggggcatt tggtgctttc gactttgcac
     1141 accaatgatg ctgcgggcgc gatttcgcgg ttactggaaa tgggagtgca ggattatctt
     1201 ctctcgtcgt ctttactggg ggtgctggcg caacgattgg ttcggcgtct ttgcccgcag
     1261 tgtcgcagag aagtcccgtt cgcaggcctc gagggcgccg aggctgaact ggatttgctc
     1321 aatggcaatc ggatcgaaac tgtatgggaa gcggtcggat gcgacggctg caacgcgacc
     1381 ggctatcttg ggcggatcgg gatttttgag ctcctccctg tcacttcgga aatttgcaag
     1441 attatcgtgc agcgcgccga tgccggcgca atccgcaacc ttgccgtacg ccaggggatg
     1501 cgcctcctga gggaggacgg ctgggacaaa gttcgccggg gtattacaac gttatcggag
     1561 ctactacggg tcacgcgaga agaggcctga gacatttggt tttcgatttc ggatttccga
     1621 ttgaactaaa catggcccgc acagcttaga gaaccccagc caatctgcac tcacactcag
     1681 tctagatccg aaatcgccga acatgatggt tggatgagag atggcattct tccaatatcg
     1741 cgccgctgat caggccggta agctggtcga aggtgttatg gaagcagacg cggagcagag
     1801 cgtagtggcg cggcttcggg aaatgggctg tgtgccgctg cgcattgcgc tcccaacggg
     1861 taggcctgcg acaacgatac gcaacccttt gccgctcggt tcgcggcgca aggtgagcca
     1921 gcaacagttg ctccagttta cccgagagct ggcaactcta ctcggagcgg gcttgccact
     1981 tgatcgcagt ctttcgattc tcggcggctt aattgaaggc ggtgaattca gcaaggttct
     2041 gcgctctctc gtggaggccg tccgggcggg caaatcattg gccgcctcga tgggggagta
     2101 tcccgacgta tttccaaaaa tttatgtgaa catgattcgt gccggagaag cgggcggtat
     2161 tttagagggg gtgctccgat atctgaccga ctacttggaa agcactgtcg ctctcaaaga
     2221 ggacatcaaa tcggcgttga tttatccaat tatattggct agcgcggcga gcctatccct
     2281 ggtggttctg ttcgtgtttg tcataccaaa atttgccacg attttccgcg ataacggcaa
     2341 ggccttgccg gtgatcacaa agatcatcat cgaacttagc caaattctgg cacaatacgg
     2401 ctgggtgatt ttgctactac tttgcgctgc tatcgtcggg gcgctgtttt acatcagaag
     2461 ccccgaaggc cgtggcgaat gggaccggat gtgcttacgc agctggttgc tgggtgatct
     2521 ggtgcgaaag ttcgaaaccg cgcggttttg ccgtacactg gcggcattgc tgaagggcgg
     2581 cgtgcctgtc ctcgaagcgc ttggaaccgt gcaaggggtc atcggcaatc gtttgcttgc
     2641 acgggcgatc actcaagttc aggcacgcgt ccgcgaaggt aaaggcatgg cccgtcctct
     2701 gaacgaaagc ggcgtttttc caagcttggc gttgaacatg atcactgtcg gtgaggaaac
     2761 cggtaagttg gataacatgc ttgctgaagt tgcgaactac tacgatcaag aggtaaagcg
     2821 agcgaccaag agactcactt cactgctcga acctgtgctc attctcggca tgggcctaat
     2881 catcggaatt gtcgtgatct caatgctaat ggcgattttc agtatcaatg atttaccgtt
     2941 ttgacgatca aaggcttccc
//
LOCUS       DASULZ010000069         2539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1123845, whole genome shotgun sequence.
ACCESSION   DASULZ010000069 DASULZ010000000
VERSION     DASULZ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123845"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..864)
                     /locus_tag="VFY96_01435"
     CDS             complement(238..864)
                     /locus_tag="VFY96_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HEX5605143.1"
                     /translation="MRCEPADELPDGRLVVSSADRNRGPILNVLERVLPKTGLVLEIA
                     SGTGQHVVHFAQALKGLSWQPTDMDAACRRSISAWLVTADLANVRQPLDLDVRALPWR
                     VPTLDAIVCLNLIHIAPWSVATALFAGAALAVRESGLLYLYGPYSVQGRHTSPSNAAF
                     DSALRAENLEWGLRDLKEVESLAKDQGFDLAETIEMPANNFSVLFRKR"
     gene            complement(971..1282)
                     /locus_tag="VFY96_01440"
     CDS             complement(971..1282)
                     /locus_tag="VFY96_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605144.1"
                     /translation="MAEFVSPDGTKKVVVFQRDCGATTDFSTQASLLATNEKLENEGG
                     NVFSADTNHNAAPSGVGGGPELRVRWESRNRLLLQHHPSARVSKRRVILTRDITYETF
                     R"
     gene            complement(1883..>2539)
                     /locus_tag="VFY96_01445"
     CDS             complement(1883..>2539)
                     /locus_tag="VFY96_01445"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605145.1"
                     /translation="APTVKKPEDLKGKRIGAAQAGTASYHAVLLGLKHWGLDPRRDRI
                     TILQLGNQGARVASLQSGGSDAIIVNPGLGPSLKERGNNIIADFTELPIPYPQQTVSV
                     RERTLKTDAEFVEKVMKGVVAGNSFSLEPRNKERVKQILAKYLRLERVSQAEEHYQAA
                     LKVMAPKPYVDLAGITSMIEFITETDPLVAKVKPEMVINHTILKKLDDGGFFDQLTKR
                     "
BASE COUNT          537 a          726 c          714 g          562 t
ORIGIN      
        1 tgatgggctt aacgggttcg gcaaaattat cttgctcaag gttgattaaa ctgaacgagc
       61 ctgcgcaagt ggatattcgc ctaacgccat gttgagccgc gccatgaggc gcatagcgcc
      121 gaatcgcgtc ggctcgaact aaagttagcg ctcattctgc ccacccagcc gaccgattga
      181 aggcttcgtc aggacgcccc ttcggtggag tcgcagcaag cgctggtgtc cggtcatcta
      241 gcgctttcga aacagcacgc tgaagttgtt agcaggcatc tcgatcgttt cggctagatc
      301 gaagccttgg tctttcgcca acgactcgac ctctttcaaa tctcgcagtc cccattcgag
      361 gttctcggcg cgcagtgcgc tatcgaaagc agcattgctc ggcgacgtgt gcctgccctg
      421 cacggaatag ggtccgtata ggtacaagag gccgctctcg cgaacggcga gtgctgcacc
      481 ggcgaagaga gccgtcgcta ccgaccaagg cgctatgtgg atgagattga ggcagacgat
      541 cgcatccagt gtgggtacgc gccaaggcaa tgcgcgtacg tcgagatcaa ggggctggcg
      601 tacattggcg agatctgcag tcaccagcca ggcggagatc gatcggcggc aggctgcgtc
      661 catatctgtg ggttgccagc tcaacccttt cagtgcctga gcgaaatgaa cgacatgctg
      721 cccggtgccg ctcgcgatct ccagcacgag cccggttttc ggcaggacgc gttcgagaac
      781 gttcagaatg ggcccgcggt ttcggtcggc tgaggagacg accagccgac catcgggaag
      841 ctcatcagca ggttcacagc gcattgccga cctccatgag cgctaacgcg atgccgctga
      901 gccgctacag cggagatcgt tcattcagca cgatgttcgc cgtcggctca agcggcgggt
      961 tatccagctt tcacctaaac gtctcatacg taatgtccct cgtcaaaatg acgcgccgct
     1021 ttgaaaccct cgcgctcggg tggtgctgga gcagcaatcg attgcgactc tcccaacgta
     1081 cccgcagctc gggaccgccg ccgactcctg acggtgcagc attgtggttg gtgtcggcag
     1141 agaatacatt gccgccctcg ttctccaact tttcgtttgt cgcgagcaac gaagcctgcg
     1201 tgctgaagtc cgtagtcgcg ccgcagtctc gctggaacac gacgactttt ttagtgccat
     1261 cgggcgagac aaactcagcc aaagcttcgt tgccggccgt tagcaaaaat ctgatagagg
     1321 aaaatcccaa aagcaatgac aaccaccgcc gtcaccgtca cggatataac gaaagccttt
     1381 ctcattcggg gagtcatttg tctggataac tactaagtga accgcgtcga attccggcgg
     1441 gctattctgg aagtcgtaga ctccgtaaac attaaatcag cagcaatatc ggtcaactcg
     1501 ctagccattg tcaatcgtct ccgcggacaa acaacgcgcc tgcggcgggt tatccggata
     1561 tcagcccgcg gtatagcccg gcaacgctag ctgtttttcg atctgaaatc ccgagtacgt
     1621 tttttcggat ttttctctga ccctcgcgtg catctgctca cacacaggac ttgcttcgta
     1681 gtaacctcac agtcggtccc tgtagcgccg agcgcggacg tttgccagac ctgagaaact
     1741 tggggctgtt gggccgttaa aaactgattg agacaacttg cgcgagccca tggtgtgggc
     1801 tgccggagga tggaaaaaca gcggagattc tattcttagg gaactgacga gacaaagtta
     1861 atcgcaatcg aaatgatccc gctcaccgct tcgtcaactg atcgaagaac ccgccgtcgt
     1921 cgagtttctt cagaatcgtg tggttaatca ccatctccgg tttcactttg gcgacgagcg
     1981 ggtcggtttc ggtgataaat tcaatcatgg aagtaatgcc ggccagatcg acgtagggct
     2041 tgggcgccat caccttgagc gccgcctgat aatgctcctc ggcttgggag actcgctcca
     2101 aacgtaagta tttggcgagg atctgtttga cgcgctcttt gttgcgcggc tcaagactga
     2161 aactgttgcc ggcgacgacg cccttcatca ccttctcgac gaattcagca tccgttttga
     2221 gcgtacgctc tcgcacgctc acggtttgct gcggataggg aattggcagc tcggtaaaat
     2281 cggcgatgat attgttaccg cgctctttca acgacggacc gaggccgggg ttgacgatga
     2341 tcgcgtcgct gccgccggat tgcagtgagg ccacacgcgc tccctgatta cccaactgca
     2401 agatcgtgat ccgatcccgg cgtggatcga gcccccagtg tttgagcccg agcagcaccg
     2461 catgataaga agcggttccc gcctgcgcgg cgccgatccg tttgcctttg agatcctcag
     2521 gttttttgac cgtcggcgc
//
LOCUS       DASULZ010000070         4161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_717760, whole genome shotgun sequence.
ACCESSION   DASULZ010000070 DASULZ010000000
VERSION     DASULZ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717760"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..983
                     /locus_tag="VFY96_01450"
     CDS             78..983
                     /locus_tag="VFY96_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX5605146.1"
                     /translation="MPELPDVTVYIEALGERVLDQTIQRIRIGSPFVVRTFDPPIKEV
                     ERKKILALRRIGKRIVIELEDDLFLIIHLMIAGRLHWKTRGAKIARKYGQAAIDFPNG
                     TLLLTEAGTKKRASIHLVRGEAALEEYDPGGLELFDMTLEQFTEALTRENHTLKRSLT
                     DPHLFSGIGNAYSDEILHRAKLSPVRLTKQMAAVEIERLYQAVRESMIEWIDRLRQER
                     GTGFPEKVTAFRRDMAVHGKYGKPCPVCGSPVQRIVHAENETNYCATCQTDGKLLADR
                     SLSRLLKQDWPRSLEELEEMKRVRS"
     gene            complement(1057..1662)
                     /locus_tag="VFY96_01455"
     CDS             complement(1057..1662)
                     /locus_tag="VFY96_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017741875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX5605147.1"
                     /translation="MSVKLYNVAYSGNSYKVRLLLSHLGVRCEFIDVDILKGESRTAE
                     FLKINPNGRTPVLDDNGFILAESNAILAYLAKGTKFLPEDRKEFGLVCQWLFFEQYSH
                     EPYIATSRFWLQHKPDSPEKTALLASKRDGAWAALKVMEEHLFRNAFFVGDYTIADIA
                     LFAYTHVAYEGGFPLDHLPKIRAWIERVRAQPGFVPICKTV"
     gene            complement(1794..2930)
                     /locus_tag="VFY96_01460"
     CDS             complement(1794..2930)
                     /locus_tag="VFY96_01460"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605148.1"
                     /translation="MTSNGTAPVGVSKNIKQLGRMDLPGSGSIVVENDYAYVGHMDPP
                     HGTSIIDVKDPKNPKLVARLEIPEGLHSHKVRVSGDIMLVNYERYKGKQPLQAGLKIF
                     DISNRAKPREIYFYETAGKGVHRFTFDGRYAYISPTVEGYNGNIAMMLDMKNPEKPEE
                     VMRWWMPGQWTAGGETATWHGTATRCHHPIRRGDRLYISYWHGGFAIVDISDLSKPKT
                     ISTLDWSPPYPCPTHTTLPMLNKIMERDWLIVTDEEVGEKLNETHNAFLWVVDITKEE
                     LPLPVATFIVPFDGKSTNEFRFGAHQPAEQVYGNTLYVTWFGGGLRAVDFSNPYMPKE
                     VGYYIPNPGKGQRVVMSNDVFHDKDGKLYLMDRYDGLEILEPQI"
     gene            complement(3027..3662)
                     /locus_tag="VFY96_01465"
     CDS             complement(3027..3662)
                     /locus_tag="VFY96_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEX5605149.1"
                     /translation="MNVNDFSLTKTGLLFFDILNGYVAEPEPGKPRVLKPWIQNAVKL
                     GKAGRAAGLPIFFAKGNHRPDNATTALLLTDTNNSLQPWPNGEVTKTKMHVIAGEKSS
                     EVLADLEPHPNDYYIVKYRWSAFFQTYLDLALRTRGIDTIIVSGGSTDVGVTSTLYSG
                     RDLDYNMIVVSDACGTSHDQRAHDMLMELVFPRMARVRTTEQVLAMLKNAR"
     gene            complement(3674..>4161)
                     /locus_tag="VFY96_01470"
     CDS             complement(3674..>4161)
                     /locus_tag="VFY96_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEX5605150.1"
                     /translation="PPRELAPTIIAAAENGKVALIFGPEDHGLSNRDLEHCQLLVTIP
                     THPDYPSLNVAQAAVICLYELYLASLTDQENADIKRADAEQIERLFDIMRNSLLKIGF
                     LDSENPEHMLLAFRRIFGRAGLEEKDVRILTGMFRQIEWYAKEGWQTVQDKEQRGIKI
                     R"
BASE COUNT          917 a         1080 c         1168 g          996 t
ORIGIN      
        1 tcggtttctc ggatgtgcgt gtttgagtcg tccggtccag gacgtttgtt ctccagtgtt
       61 gcttgtagtt cttggtcatg cctgagttac ccgacgttac ggtctacatc gaagcgctcg
      121 gcgagcgggt gctcgatcaa accatccaaa gaattcgtat cggcagcccc tttgtcgtgc
      181 gaacttttga tccgccgatc aaggaagtcg aacgcaagaa aattctcgcg ctccgtcgga
      241 tcggcaaacg gatcgtcatc gagctcgaag acgatctatt cttaataatt catttgatga
      301 tcgccggcag attgcattgg aagacccggg gggcaaagat tgcgcggaaa tatggacagg
      361 cggcgattga ttttccgaac ggaactttac tcctgacgga ggccgggacg aaaaagcgtg
      421 cgtccattca tttggttcgc ggcgaagctg ctctggaaga gtacgatccc ggcgggctgg
      481 aactcttcga tatgactctc gagcaattca cagaggcgct cacccgagag aaccataccc
      541 tgaagcgctc gctcaccgat ccccatctgt ttagcgggat cggcaatgcc tattccgacg
      601 agattcttca tcgcgcgaag ctatcaccgg ttcgcttgac caaacagatg gcggcggtgg
      661 agatcgagcg actgtatcaa gccgtgcgcg aatcaatgat tgagtggatt gacaggttgc
      721 gacaagagcg cggcaccggc ttcccggaaa aagtcaccgc atttcgccgc gacatggccg
      781 tccacggcaa gtacggcaaa ccctgtcctg tgtgcggctc tccggtgcag cggatcgtgc
      841 acgcagagaa tgaaacgaat tactgcgcaa cctgccagac tgacggcaag ctgctggccg
      901 accggagcct ctcgagactg ctcaaacagg attggccgcg aagtttggag gaattggaag
      961 agatgaagag ggtgcgcagc taaaaatagc ccatggtgcg caagcgcagc ctacgaatat
     1021 ggagattgag ccggtagggt gcgctcgcgc accatcctag acggttttac aaatcgggac
     1081 aaaaccgggt tgcgctctca ctcgttcgat ccatgctctg atcttcggaa ggtggtccaa
     1141 tgggaagccg ccttcgtacg ccacgtgcgt atacgcgaac agggcaatat cggcgatggt
     1201 atagtcaccg acgaagaaag catttctgaa caaatgctct tccataactt tcagcgctgc
     1261 ccaggcgccg tcgcgcttgg atgcgagcag cgctgtcttc tccggcgaat ccggcttatg
     1321 ttggagccaa aatcgcgagg tagcgatgta aggctcgtgg ctgtactgtt cgaagaacag
     1381 ccactggcaa actaggccga attctttccg atcttctggc aggaattttg tccctttggc
     1441 aagataggcg agaatggcgt tggattcggc gagtatgaag ccgttgtcgt cgagcaccgg
     1501 agtgcgaccg ttggggttga tcttgagaaa ttctgcggtg cggctttcgc ccttgagaat
     1561 atcgacgtcg ataaactcac atcgaacccc gaggtgagag aggagcaggc ggactttgta
     1621 agaatttccg gagtaggcga cgttgtatag cttcacactc ataaggttca aaccgtttaa
     1681 accgttcaag cggttcaaac tgtttattcg agcgcctttg ggttcactcc ggacatacgg
     1741 cttgaacggc ttaaactact tgaacggttt gaaccagtta aaccattgtt caatcagatc
     1801 tgtggctcaa gaatttccaa cccgtcatag cggtccatga gatagagctt gccgtctttg
     1861 tcgtgaaaga catcgttgct catgacgact ctctggcctt tgccgggatt tggaatgtag
     1921 tagccgactt ctttgggcat gtacggattt gagaaatcga cggcgcgcag accgccgccg
     1981 aaccaggtga cgtagagagt attgccgtag acttgttccg caggttgatg cgcaccgaag
     2041 cggaactcgt tggtcgattt tccgtcgaag ggcacgatga acgtggcgac cgggagcggc
     2101 agctcttcct tggtgatgtc gacgacccat agaaaagcat tgtgcgtttc gttcagtttc
     2161 tcgcccacct cttcgtcggt gacgatcaac cagtcgcgct ccataatttt gttcagcatc
     2221 ggcaaagtgg tgtgcgtcgg gcagggatag ggcgggctcc agtcgagggt gctgatagtt
     2281 ttaggcttac tcaaatcaga gatgtcgacg atggcaaaac cgccgtgcca ataactgatg
     2341 tagaggcgat caccgcgccg aatcggatga tggcagcgcg tcgcggtgcc atgccacgtc
     2401 gccgtttcgc ctccggcggt ccactgcccc ggcatccacc agcgcatgac ctcttccggt
     2461 ttttcgggat ttttcatatc caacatcatg gcgatgttgc cgttgtaccc ttccacggtt
     2521 ggcgagatgt aagcgtagcg gccgtcgaag gtaaagcggt gaacgccctt gcctgccgtc
     2581 tcataaaaat aaatttcgcg cggtttcgcg cgattcgaga tgtcgaaaat tttgagtccc
     2641 gcttgcagcg gttgcttgcc cttgtagcgc tcgtaattga ccagcatgat gtcgccgctg
     2701 acgcggacct tatgcgaatg cagaccctcg ggaatttcga gacgcgcgac gagtttggga
     2761 ttctttggat ctttgacgtc gataatggaa gtgccgtgcg gcggatccat atggccgacg
     2821 taggcgtagt cgttttcaac gacaatcgag ccgctgcccg ggagatccat gcggccgagt
     2881 tgcttgatgt tcttacttac gccgacgggg gcagtaccgt tcgatgtcat gtttcctcct
     2941 tagattcgct acggcttagg aaatttcgag aaagatctct cacttattcg agaagacagc
     3001 aggctgaacg ttctggtatg cagagctcac cgcgcatttt tcagcattgc taaaacttgt
     3061 tcggtcgtcc gcacgcgcgc catgcgcgga aacaccagtt ccatcagcat gtcatgggcg
     3121 cgctggtcgt gcgatgtgcc gcaggcgtcg ctcacgacga tcatgttata atccaagtcc
     3181 ctgccggaat acaaagttga tgtcacgccg acgtcggtcg aaccgcccga aacaataatg
     3241 gtgtcgatgc cgcgggttcg tagcgccaga tccaaatagg tttggaaaaa agcgctccag
     3301 cgatatttga caatgtaata atcattcgga tgcggttcca aatcagcgag cacctcggag
     3361 cttttttcgc ctgcgatcac gtgcattttt gtcttcgtca cttctccgtt cggccacggt
     3421 tgcagcgaat tgttcgtatc ggtgagaagc agggcggtgg tggcattgtc gggccggtga
     3481 ttgcccttgg caaagaaaat cggcaagccg gcggcccgtc ctgctttgcc cagctttacg
     3541 gcgttttgaa tccatggttt caatacccgc ggcttcccgg gctcgggctc ggcgacgtac
     3601 ccgttcagaa tgtcgaaaaa aagcagcccg gtttttgtaa gactgaaatc attgacgttc
     3661 acgtgatctc ctcctaacgg attttaattc cgcgttgctc cttgtcctgc acggtttgcc
     3721 aaccttcttt tgcataccat tcgatctggc gaaacatacc cgtgagtatt cttacgtcct
     3781 tctcctccaa accggcgcgt ccgaaaatgc gacgaaacgc gagcagcata tgctcgggat
     3841 tttccgaatc caaaaacccg atcttcaaca gcgaattgcg cataatgtca aagaggcgct
     3901 cgatctgttc ggcgtcggct ctctttatgt cggcattctc ctgatccgtc aaagaagcta
     3961 ggtaaagctc gtaaaggcag atcaccgcgg cctgtgcgac attaagagac gggtaatcgg
     4021 gatgggtcgg gatcgtgact agtaactggc aatgttccaa atccctatta ctgaggccgt
     4081 gatcttccgg accgaagatg agagctacct tgccattttc tgcggcggca atgatggtcg
     4141 gagcgagttc ccgcggaggt t
//
LOCUS       DASULZ010000071         3423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1022130, whole genome shotgun sequence.
ACCESSION   DASULZ010000071 DASULZ010000000
VERSION     DASULZ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022130"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="VFY96_01475"
     CDS             <1..1114
                     /locus_tag="VFY96_01475"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5605151.1"
                     /translation="EHGAEVIAIEAGRAPRSPQRGQRFQFASDLESNLDRRRIAVDMK
                     HPDGVALAKKLIAKSDVVVENFSARVMASWGLDYSQMREIRRDIIMASLQAFGQTGPR
                     RDYVSFGPILMSYSGMAYLWRDPEIDRPGAACQTAFPDYVAPSFGALAILAALHYRAR
                     TGKGQYIDISQAETAASMLGPAYLEYLVNGREPEPQGNFSAVAAPHGVYRCKGDDRWC
                     VIAVQTEEEWRRFCELADHREWLTDHRFAELPARIEYRQELDYWIENWTSKYTPHQVM
                     IMLQREGIAAAVVQTAEDLYRDPHLRERGFAREVFHRQVGWLTRVGPSVRMTEAGFVT
                     RGEAHVAGQDNEPVLSEILGMSSEEIRDLTDRQVLR"
     gene            complement(1247..2059)
                     /locus_tag="VFY96_01480"
     CDS             complement(1247..2059)
                     /locus_tag="VFY96_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5605152.1"
                     /translation="MAETKFLNANIDENTGIAWLTLNRPEKKNALSIALLGELAAVLR
                     SLAEDEKIRCIVTTGAGDSYSSGRDLYDMRGQDNRRRNRGFGGVAEIVDIMRKLPQIT
                     VAKVRGWCLGGGLALINGHDLVISADTASFGMPEVIRGSYGATATPSLFHAGIPFKKA
                     FYISLTGRNLTGIEAERVGLVSQVVPEKELDGSVEILAKELASRNGATLENAKIAAYM
                     QKDLPFDMALRADDLVQHRLRYYTNPLSDVEGYLHSQKGGGTVKYVKPEDRK"
     gene            complement(2211..3224)
                     /locus_tag="VFY96_01485"
     CDS             complement(2211..3224)
                     /locus_tag="VFY96_01485"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5605153.1"
                     /translation="MNPTFTIIDADAHHLDGPAYKNYLPERYRTRSGPYFPAFGWDIF
                     LNGTTGRKPTNPQEYCKDLDVEKIGDAVAYPSNALAIGLVRELDLATELAKAYNNWAA
                     DFCQESGNRVKYAAVIAPQVISEAVTELRRAITQKGAVAVMMPTYVQSGLDLGQPQFD
                     PIYAAAQELDVPVGFHATAQVAVGNTRFHKYLGVHMTSHPFEQMLSMVAIISNGVLDR
                     FPKLRVGFLEAGVGFLPYWMERFDEKYLLRRSEMEPLKLLPSEYIKDHRCYFTCEGEE
                     SALPLVIEQFGDECMMYASDYPHWDMEWPHSVNQVVSRKNISDISKQKILADNAKIFY
                     RLS"
BASE COUNT          777 a          896 c          964 g          786 t
ORIGIN      
        1 tgaacatggc gcggaagtca tcgcgatcga agcaggtcga gcgccgcgga gtcctcaacg
       61 agggcaaaga ttccagtttg cttcggatct cgaaagcaac ctcgatcgca gacgcatcgc
      121 cgtcgacatg aagcatcccg atggcgtggc gttggcaaaa aagctcatcg ccaagtccga
      181 tgtcgtagtg gaaaatttta gcgcgcgagt gatggcgagc tggggactcg attattctca
      241 aatgagagaa attcgtcgcg atatcatcat ggcgagtctt caggcgttcg gacagacagg
      301 accgcgacga gattatgtca gtttcgggcc aatcctgatg tcctattccg gtatggctta
      361 tctatggcgc gatccggaga tcgaccgtcc gggtgcggca tgccagacag catttccgga
      421 ttatgttgct ccgtcgtttg gcgcgttggc cattctcgcc gcgctgcact atcgggcgcg
      481 gaccggtaaa ggtcaatata tcgatatctc ccaggcagag acggcagcgt ccatgctcgg
      541 accggcttac ctggaatatc tcgtcaatgg tcgtgagccc gaacctcaag gcaatttcag
      601 cgccgtcgca gcaccgcacg gcgtctatcg ctgcaaaggt gacgatcgct ggtgcgtgat
      661 cgccgtacaa accgaagagg agtggagacg gttctgtgaa ttagccgatc atcgtgagtg
      721 gttgacggat catcgcttcg cagagctccc tgcccggatt gagtatcggc aagagctcga
      781 ctactggatc gaaaattgga cttcgaagta taccccgcac caagtcatga tcatgttgca
      841 acgcgaagga atcgctgcgg cggtggttca gaccgccgag gatctttatc gcgatccgca
      901 cctgcgtgag cgcggttttg ctcgagaggt gtttcatcgc caagtggggt ggctgacccg
      961 cgtcggacca tcggtccgga tgaccgaggc gggattcgtg acgagaggcg aagcccatgt
     1021 tgccggtcaa gacaatgaac cggtcttgag cgagattctc ggtatgtcga gtgaagaaat
     1081 tcgcgatctg acagatcggc aggtgctgcg ctaggcgaaa atcgcagggt cgacggcagg
     1141 aggacttcgc cgtcgctttt cccgctactt cttacaaggc cccgggttct ttattgtcaa
     1201 gtgtgattga gactgttcgc aagcgttgag gcgcgattaa agtcgctcac ttccgatctt
     1261 ccggtttgac gtacttgacg gtgccgccgc ctttttgcga atgaaggtat ccttccacat
     1321 cgcttagcgg gttggtgtag tagcgcaatc gatgctgcac gaggtcatcg gcgcggagcg
     1381 ccatatcaaa aggtaaatct ttttgcatat aggcggcaat tttcgcattc tccaaagtcg
     1441 caccattgcg gctggcgagt tctttcgcga gtatctcgac agacccgtca agctcctttt
     1501 ccgggacaac ctgcgaaacc aagccgacgc gttcggcttc aatgccggtc aagtttcttc
     1561 ccgtgagaga aatgtaaaag gcttttttga acggaattcc agcgtgaaaa agcgacggcg
     1621 tcgccgtagc tccatagctg ccgcgaataa cttcgggcat tccaaaactg gcagtgtccg
     1681 ccgagatcac caaatcatgg ccattgatca gcgccaggcc gccgccgaga caccagccgc
     1741 gcactttggc aaccgtgatt tgcggcagct tgcgcatgat atcgacgatc tcggcgactc
     1801 cgccgaagcc gcggttacgg cgacgattgt cctggccgcg catatcgtaa agatcgcggc
     1861 cggaggaata cgaatcgccg gccccggtgg tcacaataca gcgaattttt tcgtcttcgg
     1921 ccagagagcg aagaactgcg gcaagctcac cgaggagcgc gatgctcagg gcgtttttct
     1981 tttccggtcg attgagcgtt agccaggcaa tcccggtgtt ttcatcgata ttggcattga
     2041 ggaattttgt ttcggccatg atcactccag ggctaggggt tcaaatcgtt caaacggttc
     2101 aagcagttcc agtatcttgt tgcttctgat cctgaggtcg gagagatttt cgggaggatc
     2161 tctcgttcct tcgcgatgac aaaaagaacc gacaacgcgg ttattgatga tcaagagagc
     2221 cgatagaata tcttggcgtt gtcagcgagg attttttgtt tgctgatgtc ggaaatattt
     2281 ttacggctca ccacctggtt gacgctgtgc ggccactcca tatcccagtg cgggtaatcg
     2341 gaagcgtaca tcatgcattc gtcaccgaat tgttcgataa ccagaggcag agccgattcc
     2401 tcgccttcac aggtgaaata gcaacgatga tctttgatgt attcgctggg gagtagcttg
     2461 agcggctcca tctccgatcg acgcagcaag tatttttcat cgaagcgttc catccagtaa
     2521 ggcagaaagc caacgcccgc ctcgagaaac ccgactctta gttttgggaa ccggtcgagc
     2581 acgccgttgg aaatgatggc gaccatcgac aacatctgct cgaatgggtg cgaagtcata
     2641 tgcacgccca gatatttgtg aaagcgcgta ttgccaaccg cgacttgagc ggtggcatga
     2701 aagccgaccg gtacatccag ttcctgagcc gcggcgtaaa tcggatcgaa ttgcggctga
     2761 cctaaatcca gtcccgactg gacataggtc ggcatcatca ccgccacagc gcctttttgg
     2821 gtgatagcgc ggcgcaattc ggtgacggct tcgctgatca cctgaggcgc aatcacagcg
     2881 gcgtatttga cccgattacc gctctcctga cagaaatcgg cagcccaatt gttgtaggct
     2941 ttcgccaatt ctgtggcgag atcgagctcg cgcacgagac cgatggcgag ggcgttactc
     3001 ggataggcaa cggcatcgcc gatcttctcc acgtcaagat ctttacaata ctcttgcggg
     3061 ttggttggct tacgtccggt ggtgccattc agaaagatat cccagccaaa ggcgggaaaa
     3121 tacggcccgc ttcgcgtccg ataccgttca ggtaaataat ttttgtacgc cggcccatcg
     3181 aggtgatggg cgtcggcatc gatgatggta aaagtaggat tcacgacttc gagcctcccg
     3241 aggactctga aacagtttgg ggaacagctc gaacgtcttg aaccaatgaa agttccagcc
     3301 gttcaaacag ttcaaaacgt tcaagccgtc agtttcactc agatgaattg attaatcctg
     3361 taacccgtac cgttgtgcaa cgttcagaaa aaccgagccg acgaaaacgg tgccaatggt
     3421 aat
//
LOCUS       DASULZ010000072         3361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_7603, whole genome shotgun sequence.
ACCESSION   DASULZ010000072 DASULZ010000000
VERSION     DASULZ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7603"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1586)
                     /locus_tag="VFY96_01490"
     CDS             complement(75..1586)
                     /locus_tag="VFY96_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX5605154.1"
                     /translation="MKQEIFINSTPQESRIAIMEDGQLAEFLIERKADMGVAGNIYKG
                     KVARVLPGMQAAFVDIGMDKAGFLHVSDFSMEPEDVPLIGSSGDEVEFEEAPKRPPSR
                     RMPLEKQISRGEEILVQVAKDPLGTKGARVTSHISLPGRYMVFMPGTHHIGISRRIES
                     DEERKRLREIAQGLRADQGGFILRTACEGRSKREIQRDLSFLTKLWERIQKKSATAAA
                     PALIHQDLDLIVRAIRDFFTADTAQVVVDASKDHRRIIDFVYNFMPRLKSKIVLHAEK
                     TPLFEQYAIEEKITKALERRVWLRSGGYIIIERTEALTAVDVNTGRFVGKRNQEETIL
                     KTNMEATQEVVRQLRLRNVGGIIIIDFIDMEKEGNRKKVYDALKEALKRDKARTNILK
                     ISELGLVEMTRQRTRESLENQLLRPCPHCEGRGRIKSPATVAYEVLRAIRRQRTNGEA
                     DHNIVVRMHPDIANFLYDEKNNSLENLERDINRRIIIKASQDFHQEQYEITEN"
     gene            1831..2430
                     /locus_tag="VFY96_01495"
     CDS             1831..2430
                     /locus_tag="VFY96_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020478752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX5605155.1"
                     /translation="MLNTKVLILNRSYLPIHVTSVRRAFVLLYQGIARAVNEQYQTFD
                     FDSWSELSISAHDETIGLVNRVIRIPRVILLVTYDRVPKRQVRFSRYNIYARDKCTCQ
                     YCGRRLPRHELNLDHVIPRSQGGTSIWENVVCSCHECNRRKGGRTPHQACMPLVRRPM
                     KPQWTPFMQETFSMSRYKEWMPFLNTVDVSYWNTELIEK"
     gene            2677..>3361
                     /locus_tag="VFY96_01500"
     CDS             2677..>3361
                     /locus_tag="VFY96_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5605156.1"
                     /translation="MRLKRLEMTGFKSFAQRTVVEFNPGITAVVGPNGCGKSNIVDAL
                     RWAMGEQSPRHLRGQQMEDVIFNGSDSLPATGMAEVSIIFDNEDGRSLAEYSTFSEIM
                     VTRRLFRSGESEYAINKVNSRLKDVVELFLGTGVGSKAYSIVEQGKVDELVNSKPEER
                     RALIEEAAGTSKYKSRKVLAEKKLERTQQNLLRVADIVREIERQIRSMELQAKKAERY
                     RALRAEFKEK"
BASE COUNT          753 a          884 c          877 g          847 t
ORIGIN      
        1 actgcgagac tcgagcggtt ttttagcgcg cgacggctcg tttctccgag tgccgctcgg
       61 agctaagcag cgccctaatt ttcagtgatc tcgtattgtt cctggtggaa atcctggctg
      121 gccttgatga tgatgcggcg gttgatgtca cgttccagat tctccaagct gttgtttttc
      181 tcgtcgtata aaaagtttgc aatgtccgga tgcatacgga ccacaatgtt gtgatcggcc
      241 tcgccattcg tccgttgccg cctgatagcc cgtaatactt catacgcaac cgtcgctggc
      301 gatttgattc tgccgcggcc ctcacaatgc gggcacggcc gcagcaactg attttccagg
      361 ctctcgcgcg ttcgctggcg cgtcatttcc acgagtccaa gctcggagat ttttagaatg
      421 ttggtgcgcg ccttgtcgcg tttgagcgct tctttgagtg cgtcgtagac cttttttcgg
      481 ttgccctcct tctccatgtc gatgaaatca ataataataa tgccaccgac attcctcagc
      541 cgcagctgac gcacgacttc ctgagttgcc tccatgttgg tcttgagaat cgtctcttct
      601 tggtttcttt tgccgacgaa gcggcccgta ttcacatcga ccgcggtcaa cgcctcagtg
      661 cgctcgatga tgatgtagcc gccggagcgg agccacaccc ggcgctcgag cgctttggtg
      721 attttttcct cgatcgcata ctgctcaaaa agcggggttt tttctgcgtg gagcacgatt
      781 ttagacttca ggcgcggcat aaaattgtag acaaaatcga taatgcgccg gtggtctttc
      841 gatgcatcga caacgacctg tgcggtatcg gcggtgaaaa agtcacggat cgcgcgcacg
      901 atcagatcga ggtcctgatg gatcagcgcg ggcgccgcgg cggttgcaga ttttttttga
      961 attcgttccc agagcttcgt cagaaagctc aagtcccgtt gaatttcgcg cttgctgcgc
     1021 ccctcgcaag ccgtgcgcaa gatgaagccg ccttggtcgg cccttaggcc ttgggcaatt
     1081 tcccttaaac gtttgcgctc ttcgtcgctt tcgattcggc gggaaatccc gatgtgatgg
     1141 gtacctggca taaaaaccat gtagcgaccg ggcaacgaga tatgggaggt gacgcgagcg
     1201 cctttggtac caagcgggtc tttggcgact tggacaagaa tctcttcacc ccgggaaatc
     1261 tgtttttcta agggcatgcg tcttgaggga ggccgtttcg gcgcctcctc gaattccact
     1321 tcatcgccgg acgagccaat cagcggtacg tcctctggtt ccatggaaaa atccgaaaca
     1381 tggagaaagc cggccttgtc catccctata tccacaaagg cggcctgcat tccgggcagg
     1441 actcgcgcta ctttgccctt ataaatgttg ccggcgacgc ccatatcggc ttttcgctcg
     1501 atgagaaatt cagcgagttg gccgtcctcc ataatggcga tacggctttc ctgaggagtt
     1561 gaattaataa aaatctcttg tttcatcaaa gccaaacggg taacgaacag gagggagtct
     1621 gtgacgtgga atcatccaag gtcgcgggcg aatcccacaa acgcatctac aacccgctgt
     1681 gtttatagag gaaattactc gtgagatcaa gcgtgtgcag cccgttcgca gggattgttg
     1741 caattgacaa tggccgccat gctcgatatt ttaaaacaac taagtagcga ataaactatg
     1801 gcctatccgg ctaccgcccc cgtttacggc atactgaata ctaaagttct cattctcaat
     1861 cgctcgtatc tcccgattca cgtcacctcg gttcgccgtg ctttcgtgct tctctatcag
     1921 gggatcgccc gcgcggtcaa cgagcaatac caaaccttcg attttgatag ctggagcgaa
     1981 ctttcgattt cagcccatga tgagacgatt gggttagtca atcgtgttat ccggattcct
     2041 cgagtgatct tgctggtcac ctacgatcga gtgcccaagc gccaagtacg tttcagccga
     2101 tacaatatct atgctcggga taaatgtacc tgccagtact gtgggcgcag actgccacgc
     2161 catgagctaa acctggatca tgtcattcca cgctctcaag gcggtacctc gatctgggaa
     2221 aatgtcgtct gctcctgtca tgaatgcaat cggcgcaagg gcggcaggac gccccaccag
     2281 gcctgtatgc ccctcgtccg caggccaatg aagccgcagt ggaccccatt tatgcaggag
     2341 acatttagca tgtctcgcta taaagaatgg atgccgtttc tgaacacagt cgacgtgtcg
     2401 tactggaaca cagagcttat tgagaaatag ttttatttgc taacctctgc ccttggaaat
     2461 tcctttcgcc cgattctcgt ataaccaaga ctctcagaac gtccgtagtt tggcctatca
     2521 cccggtctta attggttctc ctatatcttg ggttaaaaac tttcacctcg cactgtatat
     2581 tgtgattttc cttgacacct ccacctaagc tatgaaatat tgcggctaag tttcgctctc
     2641 aatacgtctc acaacttccc tggatagaaa tgcattatgc gtctaaaacg tcttgaaatg
     2701 actggcttta agtcctttgc ccaaaggaca gtggtcgagt ttaaccccgg gattacggcc
     2761 gtggttggtc ccaacggctg cggcaagtcg aacattgtgg acgccttacg ttgggcgatg
     2821 ggtgaacaga gcccgcgtca tctgcgggga cagcagatgg aagacgtcat tttcaatgga
     2881 agtgacagtc tgccggccac aggaatggct gaggtttcta ttatttttga caacgaagac
     2941 ggccggagtc tagcggagta cagcaccttc agtgagatca tggtgactcg tcgcctgttt
     3001 cgctcgggcg aatccgaata cgccatcaac aaagtcaatt cccgcttaaa agatgtcgtg
     3061 gagcttttcc tcggcaccgg ggtgggcagt aaagcttatt cgattgtcga gcaaggcaaa
     3121 gtcgatgaac tggtcaattc gaagcccgaa gagcggcgcg ccttgatcga ggaggcggcc
     3181 ggtacgagca agtacaagag ccgtaaggtt ctcgccgaaa agaaactcga acgcacgcaa
     3241 cagaatctgt tgcgggtcgc tgatatcgtg cgggaaatcg agcggcaaat ccgctccatg
     3301 gagctgcaag ccaaaaaggc cgaaaggtat cgtgctctga gggcggagtt taaagaaaaa
     3361 g
//
LOCUS       DASULZ010000073         3612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_920847, whole genome shotgun sequence.
ACCESSION   DASULZ010000073 DASULZ010000000
VERSION     DASULZ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920847"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1329
                     /locus_tag="VFY96_01505"
     CDS             <1..1329
                     /locus_tag="VFY96_01505"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5605157.1"
                     /translation="EEVMSYTASGRTKDFPVFDCDSHIYEPPEIWDKYIPQNNREFAK
                     THFYRDADRLFLVRNGKISFRDPDKWKYPGETWHPGLTKKIIGETQPGTKEWDAKIGR
                     NQSARDPHARLNDMDAAGIDQVMIFPSTFVYLPLVENADAAYICARAYNDWAYDYCSA
                     DKKRLYPAAVLPVQSVDYAIEELRRVAKRGFKSALVRPIIAQGKYPTFPEFDPLWKEF
                     EELGLVLGMHTFPSRGEAMSPDLNARMGANRKRLFGDEDLLVYSPGQFVANIMQIMGS
                     KQAGDAAYGFIAEAMTWTGVVLMTGWLEKFPRLKVAILESNSSWLPLVLEKAETYLDL
                     YKHLGEKIGDPHEVFYKHCFIAFESDEIVTLRLWDLYENIALWSSDMPHLDASDVWEA
                     IDNMNKWKVPQNVQEKMLGGNARRLYGIEPEWVVTTAPEDYQPQTMSRYG"
     gene            complement(1429..2418)
                     /locus_tag="VFY96_01510"
     CDS             complement(1429..2418)
                     /locus_tag="VFY96_01510"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605158.1"
                     /translation="MIGKLVLIILVGALYSAGSNRGFAQEKIRIAWAGASPANAAIWV
                     LQEKKLLQKYGVEPEIISISASPIVSQALIAGEIDVSATSVATLLSSRLAGADTIMIL
                     GIVRTFVDHIITAQNITTPDQLRGKIGGVNRIGSTSDLGLRFALRRLGIDPDKDAKII
                     TAGGNPERFAALSKGVIHFTIIPEPFVRQALQLGFRDLFDIGSLKIPFWWNSVLSREA
                     IVKAKRPLLLKFTRAMIEAIYFNKTNKEEAKAIWGKYLKIADQEGLERAWHAYTAAYP
                     DNLLPTPEGVKTALDDLAPRDARAAAADPRAFVDPSLVREIEATGLVKQLYRK"
     gene            complement(2456..3190)
                     /locus_tag="VFY96_01515"
     CDS             complement(2456..3190)
                     /locus_tag="VFY96_01515"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605159.1"
                     /translation="MVETEKEYLVGTVRPTNRPEAGQELDLDTLIPAKIKFLYSTMNF
                     TRGTEEEFSTSMPGYEAKAAEFAKMGADLVRPSGAPPFMLLGYQKEQELISSWEKKYG
                     VQMFTSGQNHVHALRTLGIQKFVGATYFPEKMNNIFARYFTDAGFEVLAMEGIDAPFA
                     AVPSIPPEEILVFMKRIAAKHKNTDGIYMLGSAWKTVAIVDRLEKETGLTVVHAGPAR
                     AWETQLRLGLHHKIPGYGRLLAEMPS"
     gene            3516..>3612
                     /locus_tag="VFY96_01520"
     CDS             3516..>3612
                     /locus_tag="VFY96_01520"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX5605160.1"
                     /translation="MNRKITRLGFIGLGRMGKPMAIDLLKTGYDLA"
BASE COUNT          814 a          962 c          998 g          838 t
ORIGIN      
        1 gaggaagtca tgtcgtacac ggcaagcggt agaacgaagg actttccggt gttcgattgc
       61 gatagtcaca tttacgagcc gccggagatc tgggacaaat atattccgca aaacaaccgc
      121 gagttcgcca aaactcattt ctatcgggat gcggaccggc tcttcctggt gcgcaacggg
      181 aaaatcagct tcagagatcc cgacaagtgg aaatatccgg gtgaaacctg gcatccgggt
      241 ttaaccaaaa agatcattgg cgagacgcag ccgggaacca aggaatggga tgcaaaaatc
      301 ggcagaaacc aaagcgccag agatccgcat gcgcggctca atgacatgga cgccgccggc
      361 atcgaccagg tgatgatttt cccatccacg ttcgtttatc tgcccctggt cgagaacgcc
      421 gacgcggcat acatctgcgc tcgggcttac aacgattggg cgtacgatta ctgcagcgcg
      481 gacaaaaagc ggctctatcc ggcggcagta ttgccggtgc aaagcgttga ctacgccatc
      541 gaagagctgc ggcgcgtcgc caaacgcggg ttcaagtcgg ctctggtgcg gccgatcatc
      601 gctcagggaa aatatccgac ctttcccgag ttcgacccat tgtggaaaga atttgaagag
      661 ctcggcctcg tgctcggcat gcacaccttt ccatctcgtg gtgaagcgat gtcgccggac
      721 ttgaacgctc gtatgggagc aaaccgcaag cgtttgttcg gtgatgaaga tctgctggtc
      781 tactcacccg ggcagtttgt cgcaaacatc atgcagatca tgggttcgaa gcaggccggc
      841 gatgccgcgt acggatttat cgccgaggcg atgacttgga ccggtgtggt tctcatgacc
      901 ggctggttgg agaaatttcc gcgcttgaaa gtcgcgattc tggagtcgaa ttcgagctgg
      961 ctgccgttgg ttttggagaa ggccgaaact tacctcgatc tttacaaaca tttgggcgag
     1021 aaaattggcg acccgcatga agtgttttac aaacattgct ttatcgcctt cgagagcgac
     1081 gagatcgtca cgctgcgcct atgggatctc tatgaaaata tcgcgctgtg gtcttcggac
     1141 atgccgcatc tcgacgcatc ggatgtctgg gaggctatcg ataacatgaa caaatggaaa
     1201 gtgccgcaga acgtgcagga aaagatgctc ggcggcaacg ccagaagact gtacggcatc
     1261 gagccggaat gggtggtgac aacggcaccg gaggattacc aaccgcagac gatgtcgcgg
     1321 tatgggtaat atcaaacggt tcccagtcgt tccaaccgtt cgaattgtga aacccgacaa
     1381 attcgggcgc agcatgctgc gcccctacac ccgcgtgccg cctctcgtct attttcggta
     1441 gagctgcttg acgagtccgg tggcttcgat ttcgcgcact aagctcgggt cgacgaacgc
     1501 gcgcggatcc gcggccgcgg ccctggcatc gcgcggcgca agatcatcga gtgccgtctt
     1561 tacgccttcg ggagttggca gcaaattgtc cggatacgcc gcggtgtaag cgtgccaggc
     1621 gcgctcgagt ccttcctgat ccgcaatctt taaatatttt ccccagatcg ctttcgcttc
     1681 ttctttattg gttttgttga agtaaatcgc ctcgatcatg gcgcgggtaa acttcaaaag
     1741 cagcggccgt ttcgctttta caatcgcctc gcgtgacaac acgctgttcc accaaaaggg
     1801 gatcttcaac gagccgatat cgaagagatc gcgaaagccc agctgcagtg cttgacgcac
     1861 gaatggctcg ggaatgatcg tgaagtgaat tactcccttc gagagagccg cgaagcgctc
     1921 gggatttcct ccggcggtaa tgattttggc gtccttgtcc ggatcgattc cgagccttct
     1981 cagcgcaaag cgcagcccca aatcggatgt gctgccgatc cgattcacgc cgccaatctt
     2041 tcctctcaat tgatcgggag tcgtgatgtt ctgcgcggta ataatatgat caacaaatgt
     2101 gcgtacgatg cccaggatca ttatcgtgtc ggcgcccgcg aggcgcgagc tcaatagagt
     2161 tgccaccgaa gtcgcggaga catcaatctc acccgcgatg agcgcctggg aaacgattgg
     2221 gctggcgctg atgctgatga tttccggctc gacgccgtac ttttgcagaa gctttttttc
     2281 ctgtagtacc cagatcgcgg cattggccgg gctggcgcca gcccaagcga tacgaatctt
     2341 ttcctgggcg aaaccacggt tgctaccggc cgaatataaa gcgccgacaa gaataatgag
     2401 cacgagttta ccaatcacga tgcgttcctg agctaagcgc actaatgggc gaagtttaac
     2461 ttggcatctc cgcgagcaaa cggccgtagc ctggaatctt atgatgaagt cctaggcgca
     2521 actgtgtttc ccatgcgcgc gccgggccgg catggacaac ggtcagaccg gtttccttct
     2581 ccaatcggtc aacgattgcg acggtcttcc aggcagagcc gagcatataa ataccgtcag
     2641 tgtttttatg cttggcggcg atccgcttca taaataccag gatttcctcg ggcggtatgc
     2701 ttgggacggc ggcgaaaggc gcatcgattc cttccatggc caaaacttcg aatccagcat
     2761 cggtgaaata ccgcgcaaaa atgttgttca ttttctccgg aaaataagtg gcgccgacga
     2821 atttttggat gccgagcgta cggagtgcgt gcacgtgatt ctggcctgag gtgaacatct
     2881 gcacaccgta tttcttttcc cagctgctga tcagctcctg ttctttttga tagccgagca
     2941 gcatgaatgg cggtgcgccg ctcggtcgga cgagatcggc gcccattttg gcgaactccg
     3001 ctgctttggc ttcgtagccg ggcatggaag tgctgaactc ctcctcggtt ccgcgagtga
     3061 agttcattgt tgaatataaa aatttgatct tggctggaat caatgtgtcg agatcgagct
     3121 cctgtccggc ttccggccga ttggtcggtc gcaccgtgcc aactagatat tccttctctg
     3181 tttcgaccat aattctgcct cctcggttcg gcgaaaatga agtgatcgga tctaatcact
     3241 tgtgcagagc aagcgtcaag cgccgggatt tcatttttga ttatgagttc ttcgtgatgg
     3301 aaactaccga agtcctaatc atcagcggat agtgactcac gttcggctta gatccttcac
     3361 tttgttcgga tgacagtgct ttattttctg agcggcagcg aagaacctcg ttcggcagat
     3421 gaacttgctg cctcaaacgc agtcgactac ccttcgaaag ctggacaatc gcccggcagg
     3481 taattattat ccgaccgcca agcagcacat ttcgcatgaa ccgaaaaatc acccggctcg
     3541 ggtttatcgg cctcggccgc atgggcaaac cgatggcgat tgacttgctc aaaaccggct
     3601 atgatctcgc tg
//
LOCUS       DASULZ010000074         5342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_109313, whole genome shotgun sequence.
ACCESSION   DASULZ010000074 DASULZ010000000
VERSION     DASULZ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109313"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..391)
                     /locus_tag="VFY96_01525"
     CDS             complement(98..391)
                     /locus_tag="VFY96_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605161.1"
                     /translation="MAQFLSQKEIDAGVLRSVTLAQIDEIKPRRLASVVDEWKKLTKS
                     DAPPSLAVTIVYYDYLGKIRRRSQNSLRRRAMLYNSDRRIRAGWRKFCKKPQT"
     gene            1100..1759
                     /locus_tag="VFY96_01530"
     CDS             1100..1759
                     /locus_tag="VFY96_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012463808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HEX5605162.1"
                     /translation="MKSTNYFFAMVVLAWSLLPAIAYADLQEDVERAASIIERFEEIP
                     ETAIPPRVLRDAKGVAILTLTKAGFIVSGRGGTGVVVARTEKGWSGPLAIGSGGIGVG
                     FQAGVQVSEYVIILNTQEAVNAFSKGGNVTLGGNLSAAIGPIGRSAEAGVAPQAAIYT
                     YSRNQGIFAGVSLEGTVIATRYEANEQYYGKPVFPADILSGQVKPPEDAQKLLQLLAK
                     Y"
     gene            complement(1822..2352)
                     /locus_tag="VFY96_01535"
     CDS             complement(1822..2352)
                     /locus_tag="VFY96_01535"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HEX5605163.1"
                     /translation="MSRAQEALQIEPGLRWYALQTKPNRERDVEKRLTDLRLEVFLPW
                     MRTRRRIGSRFQWVMVPLFPGYVFCRLDMVLSGKAARYSPGVKDFLSFGSRVAEVGED
                     IILVLRERCPEGVAQIDPVSAKPGDVVRITEGPFSGLEAVFEQKLKGSERVAVLLEIL
                     GRQTRLVLPSETIAKV"
     gene            2856..4778
                     /locus_tag="VFY96_01540"
     CDS             2856..4778
                     /locus_tag="VFY96_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HEX5605164.1"
                     /translation="MEFENLIKKDRDLHQARSWRGNLLGYLEKVKADPTIPKLAHARL
                     YDTIMKPGVRDIHENSDAHVKRLYKDESIKLYDFFTDEFFGIEKTVSQIVRYFHAASL
                     KGEESRQVLYLMGPVGSGKSSLVEKLHRGLEETEPFYAIEGCPMFEEPLHLIPRHLRK
                     EFEKMLGVHIEGDLCPVCRYKLIHEYGGKYEEFPVYTAEFSKRSRIGIGVVPPVDPNN
                     QDTSVLIGSEDISKLDLYSEGDPRVLELNGALNIGNRGVVEFIEVFKNEIEYLHCMIT
                     ATQEKVVPAPGRHGLVYVDTVIVAHSNEAEWQKFKADHTNEAILDRIVVVRVAYNLRL
                     SEEVKIYQKIIRNSDFRAHVSPHTLEVASMFAILSRLEPTNKCDLMTKLKLYNGEEVI
                     EKGRTKKVDVQELRDSSKREGMSGISTRFIMKALDNALSDNHAGNCINPINVREALIN
                     MVKEADLPDDMRKQYLEFLQDTLHKEYLEILEKEITRAFVYSYQEQAEALFQNYLDHA
                     EAYVNKSKVKDRNTKEELSPDEGFMKSIEEQIAIIGSAADGFRQEVIAYLWAANRRGE
                     KIDYRSYEPLKEAIEKKLISSVRDLSRVITKARMRDEEQSEKYDAMVKNLLEHGYCPS
                     CVDVVLKYAANNLWKD"
     gene            4823..>5342
                     /locus_tag="VFY96_01545"
     CDS             4823..>5342
                     /locus_tag="VFY96_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HEX5605165.1"
                     /translation="MATIFRPYNSSSGQRSDRSAGDRLRHRQKVREAIRNNIADIIAE
                     ESIIGKDKNRIIKVPIRGVKEYRFIYGDNAPGVGQGGDGEPNPGDVVGEGKDGQGAQQ
                     AGDRPGIDYYETDVSLDELIDLMFEDLELPDIERKRLRQIEVERQFRHKGFRRKGIRV
                     RLDKKRTARSRVK"
BASE COUNT         1300 a         1458 c         1425 g         1159 t
ORIGIN      
        1 tcgtacattt ttttgagtga tttgatatcg gccggctcaa agcttgcgat gtaggctccg
       61 tcccactggt tcgcgtagcc tcgcgcatcg gctgcattca tgtttgcggc tttttgcaga
      121 atttccgcca cccggccctt attcttcgat ccgaattgta gagcattgcg cgtcgccgca
      181 aggaattttg cgaccgcctc ctgatttttc cgaggtagtc atagtaaacg atggtcaccg
      241 cgaggctcgg cggtgcatcg ctcttggtta gttttttcca ttcgtcgacc acgctggcca
      301 gacgacgcgg tttgatctca tcgatttgcg ccagcgtgac ggagcgcagc acgccggcat
      361 cgatttcttt ctggctcagg aattgcgcca cgcgttccct gccacacatt agtgatgatc
      421 aaaggtcgcc gtcatgaaca ctacaaagat ggtcgtcgca ttggagatgc cgaaccaata
      481 atcgcaaaaa tcgacaaaac cgtgttcaac acaagaagca ccggttcgaa gaactcaccc
      541 gctcgcctgt tggcggccag caaaattccc agtggcaagc gggtcagcgc cgccaatacg
      601 aaccccaccg aaacgcgcca cagtgtaata gcgaggttgt tccacaaatc accgtttgcg
      661 gcgatcagcc gaagcgcatc ccaaacgcgc actggcccag gtaaaatata gtgtggcagg
      721 gacagtggcg atagcgacgc ttaccaacgc gaggcgctct ccgaataagt caaagcgcaa
      781 cttacgccac gtcggctgcg ccggtaactc ggagttcatc agtaaacaga ttacaaccag
      841 gaacttcgct gatccgtctg tcttggaccg cgaatatact cccccggatc atatcaatag
      901 atctatggag ccacggttcg agtgcgcaat tgtaacgacg ttgagcaaga gggcaatttc
      961 agcctgcggc cgatgcgttt attcttagta gctttttcgc ctcacatagt tgtgagcccg
     1021 ctcgctttgc tttactgtaa ggtcctgcga ttgcttagca gcacactcaa acgggatcga
     1081 agcagcggag gaagtgcaga tgaaatcgac aaattatttt tttgccatgg tcgtgctcgc
     1141 ctggtcgtta ttaccagcca tcgcctatgc tgatcttcag gaagatgtcg agcgcgcagc
     1201 atcaatcatc gagcgtttcg aggaaattcc ggagactgca ataccaccgc gagtgctgcg
     1261 agacgcgaag ggagtagcaa tcctcacttt gaccaaagcg gggtttattg tcagcggcag
     1321 gggtggtacg ggcgtggttg tagcgcgcac ggaaaagggt tggagcggtc ccttggccat
     1381 cggctcgggc ggcatcggag tcggctttca agcgggagtg caagtctcgg agtacgttat
     1441 catcctcaat acgcaggagg cggtaaatgc gttttcgaaa gggggcaacg ttacattagg
     1501 gggcaattta agcgcggcga tcgggccaat tgggcgctcg gccgaagccg gcgtggcgcc
     1561 gcaggcggcg atttatacct acagccgcaa tcaaggaatt ttcgccggtg tttcgctgga
     1621 aggtacggtc atcgctacac gctacgaagc taatgagcaa tactatggta agccggtatt
     1681 tcccgcagat attctttccg gccaagtaaa accgccggag gacgcgcaaa aattgcttca
     1741 gcttttggcc aaatactaag gccgctccgc caggagattc tcacatttca ggacgcgtat
     1801 cgcagagcaa ccagctgacg gttacacctt agcgatggtc tcgctgggca gcacgagacg
     1861 cgtttgccgt ccgagaattt ccagcaatac cgcaacccgt tcactgcctt tgagcttttg
     1921 ctcgaagacg gcttcgagtc ctgagaatgg tccttcagtg atgcgcacga cgtcccccgg
     1981 cttcgccgaa accggatcaa tctgcgccac cccctctgga caacgctccc gcaaaacaag
     2041 aataatgtct tcgccgactt ccgccactcg gctgccgaag ctcaaaaaat ccttaacgcc
     2101 gggcgaataa cgcgcggcct tgccggacaa caccatatcc agacgacaaa acacgtaacc
     2161 tggaaataac ggcaccataa cccactgaaa ccgactgccg atgcgtcgcc gcgtgcgcat
     2221 ccagggcaga aagacttcga gccggagatc ggtcagccgc ttctcgacat ctctctcacg
     2281 attgggtttg gtctggagcg cgtaccatct taaacccggt tcgatttgca atgcttcttg
     2341 tgcccttgac attcgtgcga ctcccgcgag gtttgtaggt gcaaaacctg atgtcggccg
     2401 tagtttaaca aacaaatcgc cggatagtaa ccggcatctg tttttcttcg cgcgccgtat
     2461 ttgcccagcg gtgaaatttc ttgacatcgg cgaggatttg cggtgcaatg aagccgtggt
     2521 ggctgtcgat tgaagatgag ttgatgtttt tcgtctaaaa agcgagaggc gcttgaccga
     2581 ccgaacggat cctaaggagg tcgcatgcaa gccgagcggc acccccgatt ttctggccaa
     2641 ctgatttcca ctttcgggcc ttctccccgt ccgatttctt acgccacgtg atgcacgagc
     2701 tcaacatact gtctgcgccc gtttcgccta ctttagaacg cgtcctctgt ttgtttgcgg
     2761 ttgttttcgc ccatgaaccc acgggtctgg gcacgatcgc gccaatctcg cccgtagcga
     2821 gctgaataag ggaggctgtt ggaatggacc acaatatgga atttgaaaat ctcatcaaga
     2881 aagatcgcga cctgcaccag gcgcgctcgt ggcgcggcaa tttactgggc tatctcgaga
     2941 aagtgaaagc cgatccaacg atccccaaac tcgctcatgc gcgtctatac gacacgatca
     3001 tgaaaccggg ggtgcgtgat attcacgaga atagcgatgc ccacgttaaa cgtctctata
     3061 aagacgaatc gatcaagctg tacgattttt tcaccgacga gtttttcggc atcgaaaaaa
     3121 ccgtttcaca gattgtgcgg tactttcatg ccgcctcgct caaaggcgaa gagagccgcc
     3181 aggttctcta cctgatgggt ccggtgggct ccggcaaaag ttcgctggtg gaaaaactcc
     3241 accgcggatt agaggaaacc gagcccttct acgcgatcga aggctgcccg atgttcgagg
     3301 agcctctcca tctgattcca cgccatctgc gcaaagaatt cgaaaagatg ctcggcgttc
     3361 acatcgaggg tgatctctgt cccgtctgcc gctacaaact gattcacgaa tacggcggta
     3421 agtatgaaga gtttccggtc tacaccgcgg aattttccaa gcgctcgcgc atcggcatcg
     3481 gtgttgtgcc tcccgtcgat ccgaacaatc aggacacatc ggtgctgatc ggcagcgaag
     3541 atatctctaa gctggatctc tattccgagg gagatccgcg cgtcttggag ttgaacggcg
     3601 ccctcaatat cggcaatcgc ggcgttgtgg agtttatcga ggttttcaaa aacgagatcg
     3661 agtacctgca ctgcatgatt accgccactc aggaaaaggt cgtgcccgct ccggggcgac
     3721 atggccttgt ctatgtcgat accgttatcg tcgcccattc caacgaggcc gagtggcaaa
     3781 aatttaaggc cgatcacacc aacgaagcga ttctcgatcg tatcgttgtg gttcgcgtcg
     3841 cctataacct gcggctctcc gaggaagtaa agatttacca gaagatcatt cgcaactcgg
     3901 acttccgcgc tcatgtctca ccacacacgc tggaagtcgc gtccatgttt gcgatcctct
     3961 cgcgcctgga accgacgaac aagtgcgact tgatgaccaa gctcaaactt tataatggcg
     4021 aagaggtaat cgagaaaggc cggaccaaga aagtcgacgt gcaagaactc cgcgatagca
     4081 gcaaacgcga aggcatgtcg gggatttcca cgcggtttat catgaaggct ctcgataacg
     4141 ctctttcgga caatcacgcc ggcaactgca tcaacccgat caacgtgcga gaagccttga
     4201 tcaacatggt caaggaagcg gacctgccgg acgacatgcg caagcagtat ttggaattcc
     4261 tacaggacac cttacacaag gagtatctgg agatcctgga aaaagagatc acacgcgcct
     4321 tcgtctattc ctaccaagaa caagcggaag cgttgttcca gaattatctg gatcacgctg
     4381 aagcctacgt caacaaaagc aaagtcaaag atcgcaacac caaagaagaa ctgtcgcccg
     4441 acgaaggctt catgaaatcc attgaggagc agatcgcgat catcggctcg gcggcggatg
     4501 gttttcgcca ggaagtgatt gcctatctct gggcggccaa tcggcgcggc gagaaaatcg
     4561 attatcggag ctatgaacca ttaaaagaag caatcgaaaa gaaactcatc agctcggtgc
     4621 gcgatttgag ccgtgtcatt accaaggcgc gcatgcgcga cgaagaacaa tcggaaaagt
     4681 atgatgcgat ggttaaaaac ctgctcgagc acggttattg ccccagttgt gtggatgtgg
     4741 tgctgaagta tgcggccaat aacctctgga aagattagcg ccgccgctca gaaaaacaca
     4801 atcgagcgtt ccaggtaatc ctgtggcgac tatttttcgt ccctacaatt cgtcgtccgg
     4861 ccaacgctcc gatcgcagcg cgggagaccg cctgcgccac cgtcaaaaag tgcgcgaggc
     4921 gattcgcaac aacatcgccg acatcatcgc cgaggaatcc attatcggta aagacaaaaa
     4981 ccgcatcatc aaagtcccta tccgcggtgt caaagaatat cggttcattt atggcgataa
     5041 tgcgcctgga gtcggccaag gcggcgatgg agagccgaac cccggagatg tcgttggaga
     5101 aggaaaggac ggccaaggcg cgcaacaagc cggcgatcgt ccgggcatcg attactacga
     5161 gaccgacgtc agcctggacg aattgatcga cttaatgttt gaagatctgg agctgccgga
     5221 tatcgagcgc aaacgcttgc gccagatcga ggtcgagcgc cagtttcgtc ataagggatt
     5281 ccgccgcaaa ggcattcgtg tgcgcctcga caaaaagcgc acggcgcgtt cgcgcgtcaa
     5341 ac
//
LOCUS       DASULZ010000075         3260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1124460, whole genome shotgun sequence.
ACCESSION   DASULZ010000075 DASULZ010000000
VERSION     DASULZ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124460"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1454)
                     /locus_tag="VFY96_01550"
     CDS             complement(<1..1454)
                     /locus_tag="VFY96_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX5605166.1"
                     /translation="MPVISDQADREQIVSALDETLIVEAAAGAGKTTELVRRIVRVIE
                     TGRAEVTEIVAVTFTEKAAGELKLRLREALEETRGASTSGSEERNRLDRALGRLEEAQ
                     VSTIHGFCADLLRERPVEARIDPLFTVLTEAQASRIYDEAFHHWLEAQLADPSDGVKR
                     ALRRPSFGGFGADMDGGNGPTDRLRRAGWELLQWRDFEGEWQRPQFDRASRIGELVRQ
                     LNQLAELLESPQSSSDPLHADCWAARKLADEICRTESVAARDHDRIEAGLIELIHNRN
                     FKDPRKGNGKNYRNGVLREQVWRAHESLLHGLILFETDANADLAAALRQELRGCVRAY
                     ELAKSKAGVLDFLDLLLKTRDLLRDNSTVRASFQNRFKRIFVDEFQDTDPLQTEILLL
                     LAAADPVLTNWRETRPVAGKLFLVGDPKQSIYRFRRADVGLYRGVYEILETSGARRVT
                     LRTSFRARPNIQRAINAAFAPVMNGDGDSLQAAYV"
     gene            complement(1454..>3260)
                     /locus_tag="VFY96_01555"
     CDS             complement(1454..>3260)
                     /locus_tag="VFY96_01555"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX5605167.1"
                     /translation="LAHLQSFSLPVIQELSAWPRQATWRDWLQRLEQLAPRVLRRPEF
                     VLRVLADLRPMGAVAPVTLVEVRDVLADRLGSLEVEPPLYRYGRVFVCSPEQARGRAF
                     RVVFVPGLAERLFPQKLHEDALLLDDLRRAIRRDLTLQEDRADYERLLLRLSLGAASE
                     RLYASFPRVETAEARARVPSFYALELMRAVTGRIPDHQQIELEASENSNASLAWPAPI
                     NPQDAIDDFEHDLSVLRSLMRSTDDVKGHAHYMLGLNEYLRRSASERWARARTPWSHY
                     DGLIRVTDSTRPFLESQRLGARPYSVSALQNYAYCPYRFLLSALYHLAPLEEPEPLDR
                     MDPLTKGRLFHEVLAEFFRALQQRAIPISPSSIPAVLAVVDETLTRVADNYAELLAPA
                     VERVWQDEIAGIRTDLHIWARELAQSTTWEPWLFEFGFGLAETPGRDPKSRREPVRID
                     GRFILRGAIDLIERRPGTNILRVTDHKTSKNRSARGSIIGHGQQLQPVLYSLAVEVAT
                     GCTVESARFSYCTTAGGFTEHQVPINERTRAMGIEVLEIIDRAVEAGMLPPAPAEKAC
                     SFCDFLSVCGPDQERRARRKSHKEIADLMDLRGRP"
BASE COUNT          690 a          972 c          975 g          623 t
ORIGIN      
        1 acgtatgctg cttgcaagct atcgccgtca ccgttcatta cgggcgcaaa ggccgcattg
       61 atggcgcgct gaatgttagg ccgtgcacga aaactggtgc gcagggtgac acggcgcgcc
      121 ccggaggtct ccaggatctc atagacgccg cggtagaggc cgacatcggc tcgtcgaaat
      181 cgatagatcg attgtttcgg gtcaccgact aaaaaaagtt ttcccgctac cggacgagtt
      241 tcccgccagt tcgtcaagac tggatcagcg gccgctagca gtaacaggat ttctgtctgc
      301 agcggatcgg tatcctgaaa ctcgtcgaca aaaatgcgtt tgaagcgatt ttgaaagctt
      361 gcccgcacag tcgaattgtc gcgcagaaga tctctggtct tgagcagcag atcgagaaaa
      421 tccagtacac cggctttgct tttggcgagc tcgtaagcgc ggacacagcc gcgaagctcc
      481 tggcgaaggg ctgcagccag atcggcgttg gcatcggtct caaaaagtat caggccatgc
      541 agcaggcttt cgtgcgcgcg ccagacctgc tcgcgcagca cgccgttgcg ataattttta
      601 ccgtttcctt ttcgcgggtc cttgaagtta cgattatgga tgagctcgat cagtcctgct
      661 tcaatgcgat cgtgatcccg tgccgctact gattccgttc ggcaaatctc gtcggcgagc
      721 tttctcgccg cccaacaatc cgcgtgcaat ggatcagagc tggattgtgg agattcgagc
      781 aactcagcta actggttcaa ttgtcgaacc agctcaccga ttcgtgaggc acgatcgaac
      841 tgcggccgct gccactcacc ttcaaaatcg cgccattgca gtagctccca accagctcgt
      901 cgcagccgat cagtggggcc attgccgccg tccatgtcgg cgccaaaacc gccaaaactt
      961 ggacggcgca aggcgcgctt cacaccgtcc gatggatcgg cgagctgagc ctccagccaa
     1021 tgatgaaacg cttcatcata gatccgcgac gcttgcgctt cagtgaggac ggtaaacaag
     1081 ggatcgatgc gcgcttcaac cggccgctcg cggagaaggt cggcgcaaaa accgtgaatc
     1141 gtgctgacct gcgcttcctc gagcctaccc aaggcgcgat cgagccggtt acgctcttcg
     1201 gatccgctcg tcgaagctcc tctggtctct tcgagggctt cgcggagacg gagcttcaac
     1261 tcgccagcgg ccttctccgt gaacgtgact gcgacaatct cggtgacttc ggcacgaccg
     1321 gtctcgatca cgcgcacgat gcggcgaacc agttcggtgg ttttcccggc gccggcagct
     1381 gcctcgacaa tgagcgtttc atcgagcgcg ctgacaatct gttcgcggtc ggcctggtcg
     1441 gaaatcaccg gcatcacggt cgtcctctca aatccatcag atcggcaatt tccttgtgtg
     1501 atttgcggcg ggcgcgacgt tcctgatcgg gtccgcacac cgagaggaag tcacaaaaag
     1561 agcaggcctt ctcggctggt gccggcggca gcatgccggc ctccacggcg cgatcgatga
     1621 tctcgaggac ttcgatgccc atagcgcggg tgcgctcgtt gatcggaacc tgatgctccg
     1681 tgaaaccgcc tgcggtcgtg cagtatgaaa atcgtgcgct ctcgaccgtg caaccggtgg
     1741 cgacttcgac ggcaagacta tacagcaccg gctggagttg ttggccatgt ccaataatcg
     1801 atccgcgtgc cgagcgattc ttcgaggttt tgtggtcggt gaccctcagg atattcgtgc
     1861 cgggccggcg ctcgatcaaa tcaatggcgc cgcgcagaat gaagcggcca tcaatcctga
     1921 ccggctcgcg gcggctcttt ggatcgcgcc cgggcgtctc cgcgaggcca aaaccgaact
     1981 cgaacaacca cggttcccat gtcgtgcttt gcgcaagctc acgcgcccag atgtggagat
     2041 cggtgcgaat accggcaatt tcatcctgcc agacgcgttc caccgcaggc gcgagaagct
     2101 ccgcgtaatt gtccgctacc cgcgtcagag tctcatcgac aaccgcgagc accgccggta
     2161 ttgaacttgg ggaaatcgga atggcgcgct gctgcaaggc gcgaaaaaat tcggccagca
     2221 cctcatgaaa caatcttcct ttggttagag gatccatgcg atcgagaggc tcaggctcct
     2281 cgaggggggc caggtgatac agcgccgaaa gcaaaaagcg gtaagggcag taggcgtaat
     2341 tctgcaacgc ggagacagag taaggtcggg caccaagacg ctgcgattcg agaaacggac
     2401 gtgtcgagtc ggtcacgcga atcaatccat cgtaatgcga ccacggcgtt ctagcgcggg
     2461 cccagcgttc gctggccgag cgacgcagat actcattgag cccgagcata tagtgggcgt
     2521 gacccttcac gtcatcagtg ctgcgcatga gactgcgcaa aaccgataaa tcgtgctcga
     2581 aatcgtcaat agcgtcttgc ggatttatcg gcgccggcca ggcgaggctg gcattggagt
     2641 tctccgaagc ttcaagttcg atctgctgat ggtcgggaat gcggccggtc actgcgcgca
     2701 tcagttccag ggcgtaaaac gaggggacac gcgcgcgggc ttcagcggtt tcgacccgcg
     2761 gaaatgacgc atacaggcgc tcgcttgccg ccccgagcga gaggcgcagc aaaagccgtt
     2821 cgtagtcggc gcgatcctct tgcaaggtca agtcgcgtcg gatcgcgcgc cgaagatcat
     2881 ccagcaggag cgcatcctcg tgaagttttt gcgggaacag ccgttcggcc aaaccgggga
     2941 cgaacacgac gcggaaagct cgaccgcggg cttgctccgg gctgcaaacg aacacccgcc
     3001 catatcgata gagaggtggc tccacttcaa gagagccgag ccgatcggca agcacgtctc
     3061 tgacttcgac caaggtgacc ggcgccactg cacccatcgg ccggaggtcg gcaaggaccc
     3121 taagcacgaa ctcggggcgc cggagcacgc gcggcgcgag ctgctccaag cgctgcagcc
     3181 aatcgcgcca tgtcgcttgc cgcggccaag ccgagagctc ttggatcacg ggcaacgaga
     3241 aactctggag atgagcaagg
//
LOCUS       DASULZ010000076         2587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1428685, whole genome shotgun sequence.
ACCESSION   DASULZ010000076 DASULZ010000000
VERSION     DASULZ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428685"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VFY96_01560"
     CDS             complement(<1..402)
                     /locus_tag="VFY96_01560"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605168.1"
                     /translation="MMKTLLLLIIATVLSHPAFALDRVRIAASNPNMPNLTVATAQAK
                     GFFKEENLDAEIIRMNPNVAITALASGDIDYCQLFGAVVAGAIAGLPVRIVAGFLDNW
                     PMTLIAQPELKSLKDLKGKTLGISSFGATPDL"
     gene            complement(546..1436)
                     /locus_tag="VFY96_01565"
     CDS             complement(546..1436)
                     /locus_tag="VFY96_01565"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5605169.1"
                     /translation="MPYCYPPDPNTRKPSFTPPANSCDTHFHIFGPPEQFPFVSTHEY
                     TPPAAPLEHYLKMIQVIGIERAVVVQPSVHGLDNSATLDAIASSNGRFRGVARIDDQT
                     PKSELHRLHRSGIRGVRFNLLDRPRGNVKLAVLDRCVKNIAELGWSVDLHIDMTNLIG
                     QEKRIRNMPVPVVIDHIARVKPPEGLKQSGFQLLLDLLKLEHVWVKVSGADKICDSKV
                     YGYFGLAFTEVIPYARAAIAAAPDRIIWGSDWPHSNNFSPGHTPNDGDLIDLLAAFAP
                     DESTRKKILVDNPAVLYGFD"
     gene            1595..2473
                     /locus_tag="VFY96_01570"
     CDS             1595..2473
                     /locus_tag="VFY96_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605170.1"
                     /translation="MSRFRIQSHGRLQEWVAEEKDYFTREGLEYEFLVKPIVTWSADA
                     ERVDSSPADIQRGAFESIEDGRGCSLSAACHWTVNMAASAGHGKMWGEAYSVSPSGIF
                     VSPNSPMQRPDDLADVPITVGYHSGSHYSTLQGLERFLLRDQIKLHFGGLLLDRLALL
                     VDGQVPAASLFGAPFYVAEQLGFKKLLDTTFMIGHLVSENISRDDVDRYFKALRRAQH
                     DIDLEPEAYKHYFLRELPERYHSQIDVRRFGPGERIVFELYPQEVFERTRRWIESWNL
                     FPPEQKGNAGYDIAVV"
BASE COUNT          551 a          691 c          747 g          598 t
ORIGIN      
        1 gagatcgggc gtcgctccga agctgctaat gcctaacgtt ttgcctttca ggtccttgag
       61 ggacttgagc tccggctgtg cgatcaatgt catgggccag ttatcgagga acccggcgac
      121 gattctcacc ggcaaacccg cgattgcgcc cgctacgact gcgccaaaga gctggcaata
      181 atcgatatcc ccgctggcga gcgccgtgat ggcgacgttg ggattcatcc ggatgatctc
      241 ggcgtcgagg ttttcttctt tgaaaaatcc cttcgcttgc gccgtcgcca ccgtcaagtt
      301 gggcatgttc ggattcgatg ccgcgatgcg cactcgatcc aaggcgaagg cggggtggga
      361 taaaacagtc gctatgatga gcaggagcaa cgttttcatc atttcgtcac gttagcgttc
      421 tgcacggtgg ttgttggcag atcttatcga cgttctcgac cacgcgagca tttgcgcttg
      481 gggctttgtc aactcgtcag tggatttgat catttggatt acgtggctcc tagtgtcatg
      541 gatgctcagt cgaagccata caaaacggct gggttgtcga caagaatttt tttccgtgtg
      601 ctctcgtccg gcgcgaatgc cgcgagcaaa tcaatgagat cgccgtcgtt tggcgtatgc
      661 cccggcgaga aattgttcga gtgcggccaa tcgctgcccc agatgatacg gtcaggcgct
      721 gcggcgatcg ccgctctcgc gtacgggatc acctcggtaa acgccaaacc gaaatagccg
      781 tagactttac tatcgcaaat cttgtcggcg ccgctgacct ttacccagac atgctcaagc
      841 ttgagaaggt cgagcaggag ttgaaaaccg gactgcttca agccctccgg cggttttacc
      901 ctggcaatgt gatcgatcac cactggaact ggcatattgc ggatgcgctt ttcctggccg
      961 atcaggtttg tcatgtcgat gtggagatcc accgaccagc ctagctccgc aatatttttg
     1021 acgcagcgat caagtacggc cagtttcaca ttgccgcgcg ggcggtcaag cagattgaag
     1081 cggacgccgc ggatgccgct ccgatgcagc cggtgcagct cgcttttcgg cgtctggtcg
     1141 tcaattcgcg ccacgccgcg aaatcttccg tttgagcttg cgatggcatc cagcgtcgcc
     1201 gaattgtcta acccgtgcac gctcggttgc acgaccacgg cacgttcgat gccgatgacc
     1261 tgaatcattt tgaggtagtg ttcgaggggt gctgccggcg gcgtgtattc gtgcgtcgag
     1321 acgaacggaa attgttcggg cggaccgaaa atatgaaaat gggtgtcgca ggaattggct
     1381 ggcggtgtaa agctgggttt gcgggtattc ggatcgggtg gataacagta cggcataagc
     1441 gatttctcca gcagatcttt tagcctagct caacgccgct gtccaactct gctcaacgaa
     1501 acgcccatca aacttgatct gctctctcct tgggcgtaca aagtggcaga ctcatggcca
     1561 gactgaaacg tcaacgaaag tgaggaatat acgaatgagc cgattccgca ttcagtcgca
     1621 cggacgttta caagagtggg tcgccgaaga aaaagattat ttcacacgag agggattgga
     1681 atacgagttc ctagtcaaac cgatcgtgac ctggtccgcc gacgcggaac gggtcgactc
     1741 gagtcccgcc gacattcagc gcggcgcgtt tgaatccatc gaggatggcc gcgggtgcag
     1801 cttaagtgcc gcctgccatt ggaccgtcaa tatggcggcg tcggcaggac acggaaaaat
     1861 gtggggcgaa gcctattcgg tgtcaccgtc gggaatcttc gtttcgccaa attcgccgat
     1921 gcaaaggccc gacgacctcg ccgacgttcc gatcactgtc ggttatcact caggcagcca
     1981 ttattcgaca ctacaaggac tcgaaaggtt tttgctgcgc gatcagatca agcttcattt
     2041 cggcggcttg ctgctcgatc gattggcgtt gctcgtcgat ggccaagtgc cggcggcaag
     2101 cctcttcggc gcgccctttt acgttgccga acaactcggg ttcaaaaagc tcctggacac
     2161 cacgttcatg atcggccatc tcgtttcaga aaacatcagt cgtgatgacg tggatagata
     2221 tttcaaggcc ttgcgccgcg cgcagcacga tatcgacctc gagccggaag cgtacaaaca
     2281 ttattttttg cgagaattgc ccgagcgcta tcactcgcaa atcgacgttc gtcgcttcgg
     2341 tcccggcgag cgcatcgttt tcgagctcta tccgcaagag gtgttcgagc gcacgcgccg
     2401 atggattgaa tcgtggaatc tttttccgcc ggagcaaaag ggcaacgcgg gttacgacat
     2461 tgcggttgtt tagcttagct agtgattgcg aaggcgcgct aagaagcgcg tgatgtcgtc
     2521 ctgagcgtga gccgacggat cacggcctgg acagggaaaa tcagcgctca ttggataaag
     2581 caggatt
//
LOCUS       DASULZ010000077         3336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1327678, whole genome shotgun sequence.
ACCESSION   DASULZ010000077 DASULZ010000000
VERSION     DASULZ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327678"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..1260)
                     /locus_tag="VFY96_01575"
     CDS             complement(178..1260)
                     /locus_tag="VFY96_01575"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5605171.1"
                     /translation="MRIIDADGHVLEKQIPWADLLEAPYRDRAPKEVKDNRGFSFIMM
                     EGKLTPKPVGKACSFVGAPRSHSPQPTTGMVDPRQRLKDMDLEGIDTAVLFGTSPFLS
                     LPFVEDKDLASAIARVYNNWLAQYCSADPRRLKGVAAVAIQDPTEAVKELRRAVEVLK
                     FVAVASPPISASKKNLDDPDLFPFFAEAERLNVPVCIHVGAGDGVPAATERFDHPFFT
                     HAMAHPFEQMIASLCVVAGGLLERFPRLKFAFMEAGCGWVPYWMERLDEHYEILQPTV
                     PWLKKPPSEYMKGGQMFYAFEMEEKMLPYVAEFVGAERLVFATDYNHSDSKFPHTVEE
                     VMERKDLSDSLKAKLMGENAALLYSL"
     gene            complement(1464..2492)
                     /locus_tag="VFY96_01580"
     CDS             complement(1464..2492)
                     /locus_tag="VFY96_01580"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605172.1"
                     /translation="MSRMKNFVCVQAILCACVLWSTLHGSHVAAHAAELKPEKNRLEV
                     AIAATGPLYLPVILANEAGYFSKRGVAVSLSTLSATASAQALLSGQVDIYQGGTATIH
                     ANVAGSDLIYIAASVDRSTLVLFGQKAITSFDQLRGKSVATTSVGAFGEIAIRKTAKE
                     RGMEVGKDIKLLYHKGPPDALSTFLVGNADGLIVTPPQTEMARGKGFPVIIDYYEKGL
                     KIIGPGTGVARSFVQRNPTTLKIFLMGYLDGVKRALDDPAYAKTVLAQSSKLTDQKLI
                     DDSYQEGLKVWNKDMSVDPEAIKVVLEQSTIAKAAELDLKRFYDNTLIREVNRDYASK
                     LFPGQVKW"
     gene            complement(2513..>3336)
                     /locus_tag="VFY96_01585"
     CDS             complement(2513..>3336)
                     /locus_tag="VFY96_01585"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5605173.1"
                     /translation="ENMTGAGTLIAAKYLHSSAKPDGLSFGIFNGALILSGALGNKSV
                     DFDMRELEYLGVPVQDSTVCALRKESGVTNMDQWFAAKAPIKLGGLSPGNSTSDVARV
                     LSTSLNLPIQLVEGYKGTNEIRLAADAGELHGACWAWETLKVAWANAIPAGEVNVVLQ
                     VTAKKIPELTSVPMALELAKTEEARMLLKAGAIEPAAIVRVYVTTPRTPKDRLQILRT
                     AFAKTLTDPEFIAEAKKATLDINPLTGEDVKRIVDDLFKLDPAVKNKLAGILGAK"
BASE COUNT          705 a          908 c          930 g          793 t
ORIGIN      
        1 agatccctgc gatcatgact tatcataatt tttgggcgag cttgtcgagc agccttgtta
       61 tttgacgctc gagaaggaat ggagttacgt cgaagtttgg ccgattcctt ctcctctggg
      121 agaagatcag gatgagggcg caaagcggac ggaaaggaaa agttagcggt gcgaactcta
      181 cagactgtaa agcagcgctg cattttctcc catgagctta gctttgagcg aatcggagag
      241 atccttgcgc tccatcactt cttccacagt gtgcggaaac ttcgagtcgc tgtggttata
      301 atccgtggcg aaaaccagcc gttcggcgcc gacaaattcc gccacgtaag gcagcatttt
      361 ttcttccatc tcgaaggcgt aaaacatctg tccgcctttc atgtattcgc tcggcggttt
      421 ttttaaccag gggaccgtcg gttgaaggat ctcatagtgc tcatcgagcc gctccatcca
      481 gtagggcacc caaccgcagc ctgcttccat aaaagcgaac ttcaggcgcg gaaaacgctc
      541 caataaacct cccgccacga cgcacagaga agcgatcatc tgctcaaagg gatgcgccat
      601 cgcatgggta aagaaaggat ggtcgaaacg ctccgtggcc gcagggacgc catcgccggc
      661 gccaacatgg atgcaaaccg gcacattgag acgctccgcc tcggcaaaaa acggaaacaa
      721 atccggatcg tcgagatttt ttttcgaagc tgaaatcggc ggactggcga cggcgacaaa
      781 cttgagaact tcgacggcac gccgaagttc tttgacggcc tcggtggggt cctgaatggc
      841 gactgcggcg acgcctttta gccggcgcgg atcagcgctg caatactgcg cgagccaatt
      901 gttgtaaaca cgggcaatgg cgctcgccag atctttgtct tcgacaaacg gcaaactcaa
      961 aaagggcgag gtgccgaaga gaacggccgt atcgatgccc tcgagatcca tatccttcaa
     1021 gcgctgccgg ggatcgacca tgccggtcgt tggctgcggc gagtggctgc gcggcgcgcc
     1081 gacaaaactg catgctttgc caacaggctt cggcgtcaac ttgccttcca tcatgatgaa
     1141 gctaaagccg cgattgtctt tcacttcctt gggcgcgcga tcgcgatagg gtgcttcaag
     1201 caaatccgcc caggggattt gtttctccag cacgtggcca tcagcatcga taatgcgcat
     1261 aaacacagct cctcaataaa tgcttagagg tattcagtat cagaaaccgg caacgatgct
     1321 caacagcgga tcgagaatcg cggcggcgct tgtctgactt gggtgaagag ctccgaggaa
     1381 ttgtgggttg cgcttcgctc tacgcatcct acgggatcgg atttgtaggg tgggtggagg
     1441 tgttttgtgc ttgatttcat cgattaccat ttcacctggc cgggaaacag cttcgaggca
     1501 taatcccgat tgacctcgcg gatcaaggta ttgtcgtaga agcgctttaa atccagttcg
     1561 gcggccttcg cgattgtcga ttgctcgagc acgaccttga tcgcctccgg atcgacgctc
     1621 atatccttgt tccaaacttt gagaccttct tggtaactat cgtcaatgag tttttgatcg
     1681 gtcagcttgc tactttgcgc cagcaccgtt ttggcatagg ccggatcatc gagggcacgt
     1741 ttcaccccgt caagataacc catgagaaaa atctttaggg tggttggatt cctttggaca
     1801 aaactgcgcg ccactccagt tcctggtccg ataattttga gacctttttc ataataatcg
     1861 atgatcacgg gaaaaccttt gccacgagcc atttccgttt gcggcggggt aacgatgagc
     1921 ccgtcggcgt tgccgaccag aaatgtcgag agcgcgtccg gcggcccttt atgataaagg
     1981 agcttgatgt ctttgccgac ttccatgccg cgctctttgg cggttttgcg gatggcgatt
     2041 tcaccgaagg cgcccaccga tgtcgtcgca accgactttc cgcgcagctg atcaaagctc
     2101 gtgatcgctt tttggccgaa tagcactaac gtgctccggt ccacgctggc ggcgatgtaa
     2161 atcaaatccg aacccgcaac attggcgtgt atggtcgcgg tgcctccctg gtaaatgtcg
     2221 acttgacccg acaggagcgc ctgtgccgag gccgtcgcgc tcagtgtgct gagactgact
     2281 gccacaccgc gcttgctgaa atagcctgct tcgttcgcca ggatcaccgg aaggtaaagc
     2341 ggtccagtgg cggcaatggc aacttcgagg cgattttttt cgggtttgag ctcggcggca
     2401 tgtgctgcga cgtgcgatcc atgcagtgtg ctccacaaaa cacaggcgca gagaatcgct
     2461 tgaacacaaa caaagttctt catgcgactc atcgagttta gctctccgtc gtctatttgg
     2521 ccccgagaat gccggcgagc ttgtttttga ccgccggatc gagcttgaac aggtcgtcga
     2581 caattctctt tacatcttca ccggtcagag gattgatgtc caatgtggcc tttttcgctt
     2641 cagcgatgaa ttcgggatcg gtcaaagttt tggcgaacgc ggtacgcaag atctgcaggc
     2701 ggtctttcgg cgtgcgcggt gtggtgacgt agactcggac gatggcagcg ggctcgatgg
     2761 cgccggcttt gagcaacatt cgcgcttctt cggttttagc gagctcgaga gccatcggga
     2821 cactcgtgag ttccggtatt ttcttcgctg tgacctgtaa cacgacattg acttcccctg
     2881 cgggaatcgc gtttgcccag gccaccttca atgtttccca cgcccagcaa gcgccatgga
     2941 gttcgcccgc atcagcggcg aggcggattt cgttcgtgcc tttatagcct tcgacgagct
     3001 ggatcggcag gttgagactt gtggagagca ctcgcgccac atcgctggtg ctgttccccg
     3061 ggcttagacc gccgagctta atcggagcct tggccgcgaa ccattgatcc atgttggtta
     3121 cgccgctctc tttgcgcagt gcgcacacgg tgctgtcttg taccggaacc ccgaggtatt
     3181 cgagctcccg catatcgaaa tcgacgctct tgttgccgag cgccccgctc aggatcaatg
     3241 ctccattgaa gatgccgaag gaaagcccgt cgggtttcgc gctgctatgc aaatatttgg
     3301 cggcgatcag tgtgccggcg ccggtcatgt tttcta
//
LOCUS       DASULZ010000078         3103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1124915, whole genome shotgun sequence.
ACCESSION   DASULZ010000078 DASULZ010000000
VERSION     DASULZ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124915"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VFY96_01590"
     CDS             <1..739
                     /locus_tag="VFY96_01590"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX5605174.1"
                     /translation="SRIFMTYNQTLGDVLTLAHESGHAYHGFLMRETRPFARSYPMTL
                     AETASTFAEQILINGSLDRPGIGDGFKAVMLDMELGHGAIYLLDIPVRYNFEKAFYEE
                     RASGPLSVSRLKQLMTETQRQVLGDVLESGGDDPYFWASKLHFYITGLTFYNFPYTFG
                     YLLSRGLYSMFTREGAAFLPKYEEFLRRTGSDTPENVVKQTIGADLQKPEFWSESIRS
                     LEQPLIELQALAPRVIGLHDKQVQRSN"
     gene            complement(764..2014)
                     /locus_tag="VFY96_01595"
     CDS             complement(764..2014)
                     /locus_tag="VFY96_01595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HEX5605175.1"
                     /translation="MATAEIVAIGSELLLGQIVDTNSAWMAQRLTALGVNLYFKSVVG
                     DNPGRMKEVIGRALERADIVITSGGIGPTQDDLTREIVAEVTARKLVQDAGMLAQVEE
                     HFRRRGRAMTPNNIRQSYMPEGALPVKNPNGTAPCFIVEDSRSIIYCLPGVPVEMKWL
                     FENEVEPYLRKKFHLAEVIHYRVLKVIGIGESAVDDKIGHLIANSSNPTVGVLALPGQ
                     VDVRIAAKAANREEAKKMIAPVEEEVRRLLGDAIFAADDETMEHVVGGLLRAQKKTVA
                     VYEDVTCGQLAERLQSASTEEFAAGFICNTEPAIKALLVNCRNHEKLAALLADPASLT
                     DELALSVRGQSGSDFGVALHAVTDPDTKIQNLARGQMYISLTDGKRVLRRESTTAGRG
                     AYDRSRMTLNALDLLRAALLGRTE"
     gene            2125..2877
                     /gene="surE"
                     /locus_tag="VFY96_01600"
     CDS             2125..2877
                     /gene="surE"
                     /locus_tag="VFY96_01600"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX5605176.1"
                     /translation="MVILLSNDDGIHSEGLTSLEAELGQIAEIYTVAPDRAQSSMSHA
                     LTLHRPLRVQEIAARRLAVDGTPVDCVKLSLTGLLPTRPHLVVSGINKGPNLGDDIIY
                     SGTVSAAIEGALLGIPAIAVSLVTFKDFDFRAAAGFTAELVERIAERGIPPKTLLNVN
                     VPPGEKQNIKGWRATRMGKRHYSETIVERVDPRGGKYYWIGGDDLGFADEEGTDCKVV
                     SEGYISVTPLQVDLTDYRLLQNSTLPIFRWPQ"
     gene            2874..>3103
                     /locus_tag="VFY96_01605"
     CDS             2874..>3103
                     /locus_tag="VFY96_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEX5605177.1"
                     /translation="MTKSSNRDDELSTEVRLDKWLWAARFFKTRALAAEAAAGGKVEI
                     NGERAKPSRIVRLGDRLCIRRGRFEWTVIVKDT"
BASE COUNT          649 a          917 c          866 g          671 t
ORIGIN      
        1 atcgcggatt tttatgacct acaaccaaac actcggcgac gtcctcacac tggcgcacga
       61 atccggccac gcctatcacg gctttctgat gcgcgaaaca cgtccgtttg cgcgcagcta
      121 tccaatgacc cttgccgaga cggcttcgac ctttgccgaa cagatactga tcaacggttc
      181 gctcgatcgt ccgggtattg gcgatggatt caaggcagtg atgctcgata tggaattagg
      241 tcacggcgcc atctacttgc tcgatattcc ggtacgctat aattttgaga aggccttcta
      301 tgaggaaaga gcgagcgggc ccttgagcgt ttctcgactc aagcagttga tgactgaaac
      361 tcagcgacag gtgctcggtg atgtattaga aagcggcggt gacgatccct atttttgggc
      421 gtcgaaactg catttctaca ttaccggcct gacgttttac aattttccat acacgttcgg
      481 ctatctccta agccgcgggc tttacagtat gtttacccga gaaggcgcag ccttcctccc
      541 caaatatgag gaatttctcc ggcgtacggg tagcgacacg cccgagaacg ttgtcaaaca
      601 aacgattggc gccgaccttc aaaaaccgga attttggagc gagtcgatta gaagtctgga
      661 acagccgctg atcgagctgc aagcgctggc gccaagagtc atcggtctac acgacaaaca
      721 agtccagcgg tcaaattgaa gcggccgtgt ttttacggct tagttactca gtcctgccaa
      781 gcagcgccgc gcgcaataga tcgagagcat tgagcgtcat gcgcgagcga tcataggcgc
      841 cgcgacccgc cgtcgtgctt tcccggcgga gaactctttt accatcagtc agggatatat
      901 acatttgccc gcgcgcgaga ttttgaatct tggtgtcggg atctgtcacc gcatgcagcg
      961 cgacgccaaa gtccgagccc gattgtcctc gcaccgacaa ggcaagctca tcagtcaagc
     1021 tcgccgggtc agccagaagg gccgcaagtt tctcgtggtt acggcagttc acaagcaagg
     1081 ctttgatagc cggctcggtg ttgcaaatga aaccggcggc aaattcttcc gtgctcgcag
     1141 attggagtct ctccgccagc tgaccgcaag tgacgtcctc atataccgcc acggtttttt
     1201 tctgtgcgcg cagcaatccg cccacgacat gctccatagt ttcatcgtcc gcggcaaaaa
     1261 tcgcatcgcc taataatctg cgcacctcct cctccacggg cgcgatcatt tttttggcct
     1321 cctctcgatt cgccgccttc gccgcaatcc gcacgtccac ctgtccgggc aacgccaata
     1381 cgccaaccgt gggattgctg gagttggcga tcaaatggcc gatcttatcg tcgacggcgc
     1441 tctctccgat cccaatcact ttcaaaacac ggtaatgaat cacttcggcg aggtggaatt
     1501 tttttctgag atacggttcg acttcgttct cgaatagcca tttcatctcc accggcactc
     1561 cgggcaggca ataaataatg ctgcgcgaat cttctacgat aaaacacggc gccgtcccgt
     1621 tgggattttt taccggcagg gcgccttcgg gcatatacga ttgacggatg ttgtttgggg
     1681 tcatcgcccg cccgcgccgg cgaaaatgct cctccacttg agccagcata ccggcgtcct
     1741 ggaccagttt gcgcgcggtc acttcggcga cgatctcgcg ggtcaaatcg tcctgggtcg
     1801 ggccgatgcc gccgctggtg atgacgatgt cggcgcgctc gagagcgcga ccgatgacct
     1861 ccttcatgcg gccgggattg tcgccgacca ccgatttgaa atagagattg acgccgagcg
     1921 ccgtcaatcg ctgcgccatc caggcggagt tggtatcgac gatctgaccg agcagcagct
     1981 ccgatccgat cgccacgatc tctgcggtag ccataaaaaa tctctcctcg attcggctga
     2041 ttttatatcg tagccacgcc ggcggaaaca aggcgcccag gcgcgccctt gtccgtttgc
     2101 cgacggtttg cgaaaatgac tgacgtggtt attttgcttt ccaacgacga cggtatccac
     2161 tccgagggcc tcacgtctct ggaagcggag ctcggtcaga tcgcagaaat ttatacggtc
     2221 gcgcccgatc gcgcccagag ttccatgagc cacgctctca ctttgcaccg gccgctgcgc
     2281 gtgcaggaga tcgcggcgcg tcgtctggcg gtggacggaa cgccggtcga ctgcgtgaag
     2341 ctttcgctga ccggtcttct tccgacgcgg ccccatttgg tggtctcggg catcaacaaa
     2401 gggcccaatc tcggcgacga tatcatttat tccggcacgg tttccgcggc catcgaaggc
     2461 gcgttgctcg gcattccggc catcgcggta tcgttggtga cgttcaagga ttttgatttt
     2521 cgcgccgcgg cgggattcac cgccgagttg gtcgaacgca tcgccgagcg cggcattccg
     2581 ccgaaaaccc tgctcaacgt caacgtgccg ccgggcgaga aacaaaacat caagggctgg
     2641 cgcgccacgc gcatgggcaa gcgccattac agcgaaacca tcgtcgagcg cgtcgacccg
     2701 cgcggcggca agtattactg gatcggcggc gacgatctag gctttgccga cgaagaagga
     2761 accgactgca aagtcgtgag cgaaggctat atctcggtga cgccgctgca ggtcgatctg
     2821 acggattaca gactactcca gaacagcacg ttgccgattt ttcgctggcc acagtgacaa
     2881 agtcgagcaa tcgtgacgac gagttgagca cggaagtccg cctcgacaag tggctctggg
     2941 cggcgcgctt ttttaaaacc cgagcgctcg cggccgaggc ggctgccggt ggcaaagtcg
     3001 aaatcaacgg cgagcgcgcc aaaccgagcc gcatcgtgcg tttgggcgat cgcctgtgca
     3061 ttcggcgcgg ccggttcgaa tggacagtga ttgtcaaaga cac
//
LOCUS       DASULZ010000079         2943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1226176, whole genome shotgun sequence.
ACCESSION   DASULZ010000079 DASULZ010000000
VERSION     DASULZ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226176"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VFY96_01610"
     CDS             <1..554
                     /locus_tag="VFY96_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009370160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HEX5605178.1"
                     /translation="GEEHRRFIETCKRIRLILGSHIYAEGDTTLEQRVGQLLSERHRT
                     LALAESCTGGLISHRITSIAGSSAYYLGGVTSYANDVKIELLGVRPATLEKYGAVSQE
                     TALEMSHGVREKIGADYGLSVTGIAGPSGGTPEKPVGTVWISIAQEKNHQARLFHFHG
                     DRERVILASSQAALNWLRLALFE"
     gene            558..1157
                     /gene="thpR"
                     /locus_tag="VFY96_01615"
     CDS             558..1157
                     /gene="thpR"
                     /locus_tag="VFY96_01615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX5605179.1"
                     /translation="MIRAFIAVDLDDPVIEKICNVIEILNSRITEIRWLRKENIHLTL
                     KFLGNIAESQVEPIAAALRHTLGLFSPCTISAKRLGVFPDFRRPKILWVGLTGDHLVQ
                     LAAMIESALVPFGFIPEKRAFTPHLTIGRWREGSRPPKNLRQEIDSWNDFEFGACAVR
                     QIVLFQSVLKPEGASYSQLKTIQLGAELAGNRKLELKEE"
     gene            1158..2219
                     /gene="recA"
                     /locus_tag="VFY96_01620"
     CDS             1158..2219
                     /gene="recA"
                     /locus_tag="VFY96_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX5605180.1"
                     /translation="MDVNREKALDLAVSQIERQVGKGAIMKLGEAGAPKDLPVISSGS
                     LGLDIALGIGGVPRGRVIEIYGPESSGKTTLALHIVAEAQKQGGMGAYIDAEHALDLG
                     YAKKLGVKTDDLLISQPDHGEQALEIAETLVRSGAIDVLVIDSVAALVPKAEIEGEMG
                     DAHMGLQARLMSQALRKLTATISRSHTVVIFINQIRMKIGVMFGNPETTTGGNALKFY
                     ASVRMDIRRIGALKEGDNVVGGRTRVKVVKNKMAPPFKEAEFDILYGTGISRDGEIVD
                     LGSEIGVVEKSGAWYSYAGERIGQGREAAKQFLKEHPETAKDIMAKVMDKTGIKRPET
                     PPPPAAGATADKKEKAKSR"
     gene            2309..2731
                     /locus_tag="VFY96_01625"
     CDS             2309..2731
                     /locus_tag="VFY96_01625"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEX5605181.1"
                     /translation="MGFRPRSETEVRDYLQQRGFSRTALEPAIRRLLSLNYLNDEVFA
                     RNWALSRAQSYGYGPLKIDYELRTKGIALPVIRDTLRETFDQVDETAQARRLLTKHFK
                     DERLSETRTARRAAAFLQRRGYSGKVIFDLLRCSIDDE"
     gene            2734..>2943
                     /locus_tag="VFY96_01630"
     CDS             2734..>2943
                     /locus_tag="VFY96_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011368247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HEX5605182.1"
                     /translation="MLTGNEIRKSFLEFFSRQGHTVVKSSSLVPEKDPTLLFTNAGMV
                     QFKNVFLGQERLPYVRAASAQKCLRI"
BASE COUNT          731 a          776 c          826 g          610 t
ORIGIN      
        1 ccggtgaaga acaccggcgc tttatcgaaa cgtgtaaaag aatccgcctg atactgggat
       61 cccatattta cgctgagggc gataccaccc tggagcaacg ggttggtcaa cttcttagtg
      121 aacgacatcg gacgttggct ttggccgagt cgtgtaccgg cgggttgatc agtcatcgca
      181 ttacgagcat cgcgggaagc tcggcgtact atctgggagg cgttacctcg tatgccaatg
      241 acgttaaaat cgagcttctc ggcgttagac cggcgacatt ggagaaatac ggcgcggtta
      301 gccaagagac cgccctcgag atgtcacatg gcgttcgtga gaaaattggc gccgattacg
      361 gtctaagcgt aaccggcatc gccgggccca gtggcggcac tccggaaaaa cccgtaggca
      421 ccgtctggat tagcatcgca caagaaaaaa atcatcaagc cagattgttc cattttcatg
      481 gcgatcgcga gcgcgttatc ctcgcgagct cgcaggctgc tctcaactgg cttcgcctcg
      541 ctcttttcga atagatcgtg attcgcgctt ttattgccgt ggaccttgat gatcctgtca
      601 tcgaaaaaat ttgcaacgtc atagaaattc tcaactcgcg tataactgaa attcgttggc
      661 tgaggaaaga aaatattcac ctgacgctca agttcctcgg caatattgca gaatcgcaag
      721 tcgaacctat cgccgcagcg ctccggcaca cgctagggct cttttcgccc tgcaccataa
      781 gtgctaaacg tttgggagta tttcccgact tcagacgtcc aaagatattg tgggtgggat
      841 tgaccggcga tcacttggtg caattggcgg caatgatcga atcggcgtta gtgccgttcg
      901 gatttatacc cgaaaagaga gctttcacgc cccatctaac gattggccgc tggcgcgagg
      961 gtagccggcc gccgaaaaac ttgcgccagg aaatcgacag ctggaacgat tttgaatttg
     1021 gcgcttgcgc cgtccgtcaa atagttttat ttcagagcgt gctcaagccc gaaggggcga
     1081 gttacagtca gttgaaaacg atacagctcg gcgccgagtt ggcgggcaac cgaaagctcg
     1141 aactcaagga ggagtaaatg gatgtaaatc gagagaaggc actcgatctc gccgtcagcc
     1201 aaatcgaacg gcaagtcggc aagggcgcca tcatgaagct tggcgaagct ggtgccccaa
     1261 aggacttgcc ggtgatttcc tcgggatcgc tcggactcga tattgccctt gggatcggcg
     1321 gagttcctcg tggacgagtc atcgaaatct atggtcctga gtcgtccggc aaaaccaccc
     1381 tcgcgctgca catcgtcgcc gaggcgcaaa agcaaggagg gatgggcgcc tatatcgacg
     1441 ccgaacacgc cctcgacttg ggttatgcca aaaaactcgg tgtcaagacg gatgacttgc
     1501 tgatctcgca accggaccac ggcgagcagg ctctggaaat cgcagagacc ctggtgcgga
     1561 gcggcgccat cgatgttctg gtcatcgatt ccgttgcggc gcttgtaccc aaggccgaaa
     1621 tcgaaggcga gatgggcgac gcccacatgg gactgcaggc gagactcatg tcgcaagctt
     1681 tacgcaaatt gaccgcgacc atttctcgtt cgcacacggt ggtaattttt atcaatcaaa
     1741 ttcgtatgaa aatcggcgtc atgttcggca atccggaaac tacgaccggc ggaaatgcgc
     1801 tcaaatttta cgcctccgtt cgaatggaca ttcgccgtat cggcgcgctc aaggaaggcg
     1861 ataatgtcgt cggcggccgg actcgagtca aggtagtcaa gaacaagatg gcgccgccgt
     1921 tcaaagaggc tgaattcgac atcctatacg gcacgggcat ttcgcgcgat ggagaaatcg
     1981 tcgatcttgg cagcgagatc ggtgtcgttg aaaaaagcgg cgcctggtat tcctatgcgg
     2041 gtgagcgcat tggccaagga cgcgaggccg cgaaacagtt tctgaaagaa catccggaaa
     2101 cggccaagga tattatggcg aaagtcatgg acaagaccgg gatcaagcga ccggaaaccc
     2161 cacccccgcc cgccgcaggc gcgacagcgg ataaaaaaga aaaagcaaag agcaggtaag
     2221 ctagacgggt tcactggcga acaaacctaa gcccaaatcg gagcgcgcag atagcgcgga
     2281 agcagcgctg caactggcgc tgcggtattt gggattccgt ccgcgaagcg aaaccgaagt
     2341 tcgagattat cttcagcaaa ggggcttttc gagaaccgcc ctggaacccg ccatccgccg
     2401 gctcctttcg ctcaattacc tcaacgacga agtttttgcg cgaaactggg cgctgtcgcg
     2461 cgcccaaagt tatgggtacg gcccattaaa aatcgactat gaactgagaa ccaagggtat
     2521 cgcgctgcct gtgatccgcg acacgttgcg cgagaccttc gatcaagtgg acgaaaccgc
     2581 gcaagccagg cggcttttga caaagcattt taaagacgaa agacttagcg agaccagaac
     2641 tgcccggcgc gctgcggcgt ttctgcagcg gcgaggctat agcggcaaag tcattttcga
     2701 tctgctacgc tgttcgatag acgacgaata accatgctta cgggaaacga aattcgtaaa
     2761 tcatttttgg agtttttctc cagacaaggc cataccgtcg tgaaaagctc gtcccttgtg
     2821 cccgagaagg acccgacgct gctgttcacc aatgctggaa tggtgcagtt caaaaacgtt
     2881 tttcttggac aagagcggct gccttatgtg cgcgccgcca gcgcccagaa atgtcttcgt
     2941 atc
//
LOCUS       DASULZ010000080         2525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_414836, whole genome shotgun sequence.
ACCESSION   DASULZ010000080 DASULZ010000000
VERSION     DASULZ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414836"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(334..1152)
                     /locus_tag="VFY96_01635"
     CDS             complement(334..1152)
                     /locus_tag="VFY96_01635"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HEX5605183.1"
                     /translation="MPIITIYQGASGEGQELAEAVAHDLGYRSVGREVLVEASRRYRI
                     PEAKLSDIVEKGPHWWERLLEDVRPYRIALQAALCELARESKLVYHGHLGHELLPGIS
                     HVLKVLLSAPIEYRVKQVRARQGLTDGSARKYIDELDKARSRRLMEMFGSDWRNPNRY
                     DLVLNMGKMHRESAKHIILAAANLEQYQPTPASLQAFNDLAIAARVHATLIASPHLQG
                     LSVEVRAEAGHVHVKGRIDHGLEDEIMMIVKNAPGVIKVTTDLYSVPPEALLGA"
     gene            1522..1650
                     /locus_tag="VFY96_01640"
     CDS             1522..1650
                     /locus_tag="VFY96_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605184.1"
                     /translation="MMHKMMVSAIVGGLLVGYATLLYALVHMIMVMNHSYLLSLAS"
     gene            complement(2025..>2525)
                     /locus_tag="VFY96_01645"
     CDS             complement(2025..>2525)
                     /locus_tag="VFY96_01645"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX5605185.1"
                     /translation="DPGKVYGTAPTTNCGPETPAGFCVSSDGGATWKSMTPRDFRIGY
                     VDPFFVHPRDPNLLFVAGAKTGPGTWRKLHTADARIARSRDGGKTWEILTGGLPEHLR
                     ANIEGMAMDVWNGGYAIFAGTTDGDVFYSEDGGEHWQTIIKGIGPVSKSHHYHNLALE
                     EQQAHH"
BASE COUNT          552 a          697 c          656 g          620 t
ORIGIN      
        1 agtcccgcga gcgtttcggg gagtgatcgc ttggtgacgg cgttctttaa aacctttttc
       61 ttcatcgaaa aaaaacccgg ctggaaatcc cagtccgggt aaaatgaatt cataaccgat
      121 aggcgcgacc gggagccgcc cgctctgcaa gaatcgccta tgtttagttt aattgtcgcg
      181 ctgccgtcaa ggacggaaag aactagtcag ggtggttcat tttaacgagt tattcggatc
      241 gtgagcattg gtcgtggctt tgctgaggat ttgttcgaat ttgttgccgc cagatttgtt
      301 tggatctttg gatttcgtgc ttcggattgt atatcacgct cctaaaagcg cttccggcgg
      361 gacggaatag aggtctgttg ttactttgat cacgccggga gcattcttta cgatcatcat
      421 gatttcatcc tcgaggccgt gatcaattct ccccttgaca tgaacatgcc cagcttcggc
      481 gcgcacctcc acggacaacc cttgcaaatg cggcgatgca ataagagtgg catggacgcg
      541 tgcagcaatg gccaagtcgt tgaacgcctg gagagatgcc ggtgtgggct gatactgttc
      601 gagatttgct gccgcgagaa taatatgctt cgcactttcg cggtgcattt tgcccatgtt
      661 caggacaaga tcataacgat ttgggttccg ccaatccgag ccgaacatct ccatcagccg
      721 gcggctgcgg gctttgtcaa gctcgtcgat atatttgcgg gcggagccgt ctgtgagtcc
      781 ttgccgagcg cgcacctgtt taacgcggta ttcgattggt gcactcaata gtaccttcaa
      841 aacatgcgaa atacccggca gtagctcgtg acccaagtgg ccgtggtaca caagctttga
      901 ctctcgagcc agttcacaca aagcagcctg cagcgcgatc cgatatggcc gaacatcttc
      961 cagcaaccgc tcccaccagt gtggcccttt ttcgacgatg tcactgagtt ttgcctcagg
     1021 tattcgatac cggcggctcg cttcgacaag cacttcgcgg ccgacggaac gataaccgag
     1081 atcgtgggcc acagcctcgg ccaactcctg accttcgcct gaagcaccct gataaatcgt
     1141 gatgatgggc atgaccttag ccctccttta ggctgacgct gcgtgctctg caacacaagg
     1201 aatccagggc aacaccgatg ccatcacaca tttgtgcttg gatctgcttt catgaactga
     1261 actcaattgt aaatgaaggc ttcttacgtt ttaccggctc ccgacgatcc acggtacccc
     1321 ttcagttgca gtgccgaagc aactttgttt acgccgtgta tacgcgcgtt gacagaagca
     1381 cttctccacg gctgctcgca attatctatt cgccgcaaat tcgctacttc ggtgaccgat
     1441 cgtcattggc atcgtcgttg cctatcgaaa gattgcaacc ctaaccaaca ggcgcaaaca
     1501 gaaccagact tgaggtgagt tatgatgcac aagatgatgg tctctgcaat tgtcggtgga
     1561 ctcctggtcg gatatgccac attgttatat gccctagtcc atatgatcat ggtgatgaat
     1621 cattcctact tgctgagcct tgcgagctag gacgtaccgc gccagttttc gccatctgca
     1681 atggcacagt gcatttcgat gcatgaagaa ccaaggaccg gaatgtgcgc cgattccgtc
     1741 gcggattcaa agaagcgttt ccgccgcgtc aaatttggtg cgcgctcacc ggtcgatgaa
     1801 agcctttcag tataagcttg ccaatagatc gctatccgac aaatgcctcg acggcctatg
     1861 agccgctgcg gactagatga aagattctcg ccgatcatca tttatcaatt tgtgatctgc
     1921 gccctggcgg cttggctcca tttcaatcga aaaaaattgt tcgtaaaaaa agtagaggcg
     1981 cagcttgtgc gcccctacag attaatcgca gttgcaatcg ccgactaatg atgcgcttgc
     2041 tgctcctcca gcgccagatt gtggtagtgg tgcgatttcg ataccgggcc gatgcctttg
     2101 atgatcgttt gccagtgctc gcccccatcc tcgctgtaaa aaacatcgcc gtccgtcgtg
     2161 ccggcgaaaa tagcataacc accgttccac acatccatcg ccatgccttc gatattggca
     2221 cgtaggtgtt cgggcaagcc accggtcaag atttcccagg ttttgccgcc gtcccggctg
     2281 cgcgcgatgc gggcatcggc ggtgtgaagc tttctccacg tgccgggtcc ggttttggcg
     2341 cccgcgacga acaacagatt cggatcgcgc ggatgaacga agaacgggtc cacgtagcca
     2401 atgcgaaaat cccgcggcgt catgctcttc caggttgcgc cgccatcgct gctgacacaa
     2461 aagccggccg gggtttccgg cccacagttc gtcgttggcg cggtgccgta aaccttgccc
     2521 ggatc
//
LOCUS       DASULZ010000081         2568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1125439, whole genome shotgun sequence.
ACCESSION   DASULZ010000081 DASULZ010000000
VERSION     DASULZ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125439"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..349
                     /locus_tag="VFY96_01650"
     CDS             83..349
                     /locus_tag="VFY96_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605186.1"
                     /translation="MAPVLVVTIVVVPLVVLVYYFYLPADLTTRRQVRVYVRVGRTRP
                     DSPDYLREVTGSKLLRGHAPGNDICGRNLPSDDRVSLFPRRTLT"
     gene            1020..2105
                     /locus_tag="VFY96_01655"
     CDS             1020..2105
                     /locus_tag="VFY96_01655"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5605187.1"
                     /translation="MKSRIVAQSERMKILGFALCWLFLVPSEGWVQSGKPMTITEIVT
                     YSGADREQLLYAGAKTEGKITWYTSLAGDSYKGMVKTFETKYPGVKVEAYRVSGSDMT
                     TRMMEEAKAKRYIADTVETTEGNLSFMRDAFLLRPYHSPHFKSYPEDAKEKGERGLYF
                     WAIARESYIGFAYNTKLLAKDAVPKNYDGLLHPALKGRMGVSVSDPAYKVIGAMLRTK
                     GKEFVQKLKVQEIALHTIIPPALLDLIASGEVLASPAIFRNHTLIAIAKGAPVNWVPM
                     DIVPTNVGGAAIAVQPPHPHAALLLADYLLGPEGQSVLEKYEYGSATKNLGFKRWRPD
                     HGIVTEKYEQELEGWEKLLKQISRKGS"
     gene            complement(2132..>2568)
                     /locus_tag="VFY96_01660"
     CDS             complement(2132..>2568)
                     /locus_tag="VFY96_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX5605188.1"
                     /translation="KVVPPYVDPDGYFFLADTLEELAAAIKNEYQAKPMKGTTLRATV
                     ERYNSMVDRGVDLDFGKPSPKYKIQTAPFYAAWGTVVVHDTRAGLRINAQCQVIDMNG
                     DVIPGLYCAGESAGGFNQHGMGRCATQGYIAGLNAATEEPRR"
BASE COUNT          612 a          698 c          677 g          581 t
ORIGIN      
        1 gcagggcacg cgctaaccgt gtacatgacc cctatgattg ttcctcccga acagttcata
       61 aactggagtg actaaaaatc taatggcccc ggtccttgtt gtcacgatcg tcgtcgtgcc
      121 gcttgtcgtc ttggtctatt acttctatct gcccgcggat ctcaccacca gacgacaggt
      181 ccgtgtgtac gttcgcgtag gccgcacccg cccggatagc ccggattacc tccgcgaggt
      241 caccggcagt aagctgctgc ggggccatgc tcccggcaat gacatttgcg gacgtaatct
      301 tcccagtgac gaccgcgtca gtctctttcc ccgcaggaca ctgacctgaa aggttggtag
      361 gatccttatt agtgtccgtc tggcaaagcc agataacgat gctgccgttc acacctagtt
      421 gagcaatatg gatatggctc tgggtcacga caccctgtaa tcctcggtat cttagtacgt
      481 actcgatcga ctgttcgtcg cggctgatcc gcgcgaggaa ctcgccggta gcgagagtgg
      541 agaccgatgg aacctcctca tagccggtta gatcggcacg cagccttctc tcatcatcat
      601 gcccggacgg gccggcggct gttagagaaa acgcagccat aacaagaatt aaaattcgta
      661 atcgcttcat agaacaccct ccttgtaatc cgaaaggttt ggaagccggt tttcccttcc
      721 cgtacccagt cctcagtcca gaggcgcaat accaatatcg ccgatgctcg gtcaatcccc
      781 agaaggggtg atttttatga ccccctaact gaggatcaaa gctcatcgac agtatttgtt
      841 tggggtggta tcggggcgac agttttttcg cagtccgccc atcaattcga ttatttatac
      901 ggtcaccctt ccaaggagta cagctttaag cgttggtacc cggacgccgg ccgatgtata
      961 gagcagtatg aaaaagacag cgtccattgg gaaaaattcg ttaaggagat tactcagaga
     1021 tgaaaagtcg cattgttgca caaagtgaaa ggatgaagat cctgggtttc gcgctttgtt
     1081 ggctgtttct ggtgccatct gagggctggg tacaaagcgg caagccaatg acgatcacag
     1141 agatcgtcac ttacagcggc gccgatcgag aacaattgct gtacgcggga gcgaagaccg
     1201 aaggaaaaat cacctggtac acgtctctcg cgggagattc ctacaaagga atggtgaaaa
     1261 cgtttgaaac aaaatatccg ggcgtgaaag tcgaagcgta tcgggtctcg ggctccgata
     1321 tgacgacgcg catgatggaa gaagcaaaag ccaagcggta catcgccgac acggtcgaaa
     1381 ccaccgaagg caatctctcg ttcatgcgcg acgcctttct gctgcggccc tatcattcgc
     1441 cgcattttaa atcgtatccg gaagacgcca aggaaaaagg tgagcgcggg ttgtatttct
     1501 gggcgattgc gcgcgagtcg tacattggat ttgcatacaa caccaaactt ctcgccaaag
     1561 acgccgtgcc gaaaaattac gatgggctgc tgcatccggc actcaaaggg cgcatgggcg
     1621 tttccgtcag cgatccagcc tacaaagtca tcggcgcgat gctcagaacc aaaggaaagg
     1681 aatttgtcca gaagctcaaa gtgcaggaaa tcgcgctaca cacgattatc cctccggcgc
     1741 tgctcgattt gatcgcttcg ggcgaagtgc tcgcttcgcc cgcgattttt cgcaatcata
     1801 ccttgattgc gatcgccaaa ggcgcgcccg tcaactgggt gccaatggat atcgttccaa
     1861 caaacgtcgg cggcgctgcc attgcggtgc agcctcctca tccgcatgcc gccttgctgc
     1921 ttgccgacta tcttttagga cccgaaggtc aatcggtttt ggaaaaatac gagtatggta
     1981 gcgcgactaa gaatctgggt ttcaaacgtt ggcgccccga tcacggcatc gtaacggaaa
     2041 agtatgaaca agaactcgaa ggttgggaga aattactgaa gcagatcagc cgaaagggca
     2101 gttagattgc ggagagcagc atgacgaaat cttatcgacg cggctcttct gtcgcggcgt
     2161 ttagcccagc gatatatccc tgcgtcgcac agcgtcccat gccgtgctgg ttgaagccac
     2221 cagcggactc acctgcgcaa tacaggccgg gaatcacatc gccgttcatg tctatgacct
     2281 ggcattgagc attgattctc aatcccgcac gcgtatcgtg caccaccaca gtcccccagg
     2341 cggcatagaa cggcgccgtt tgaattttgt attttggcga cggctttccg aaatcgagat
     2401 cgacgccgcg atccaccatt gagttgtagc gctccaccgt cgcgcgcaaa gttgtgccct
     2461 tcatcggttt tgcctgatat tcgtttttta tcgcggcggc gagttcttcg agggtatcgg
     2521 ccaaaaagaa ataaccatcc ggatcgacat acggcggcac cactttcc
//
LOCUS       DASULZ010000082         4751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_820999, whole genome shotgun sequence.
ACCESSION   DASULZ010000082 DASULZ010000000
VERSION     DASULZ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820999"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..607
                     /locus_tag="VFY96_01665"
     CDS             185..607
                     /locus_tag="VFY96_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605189.1"
                     /translation="MRRRAKTRAARDLFAPASEPKRTIVGIKVKFELNSKNGLRRVIF
                     GLAKSSQGDDVLWKINFALFERAKKSDPYGDALLDMDVEVDTQLSKKAEDTAQKGLTP
                     GQAAHALGPAAEDAKAAAAGEIDHEEASDTVQATLRKK"
     gene            622..2709
                     /locus_tag="VFY96_01670"
     CDS             622..2709
                     /locus_tag="VFY96_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605190.1"
                     /translation="MAYVLLLTFAIILSSRVQAASVLSAGNCPDGVDFEKSGYSIRFA
                     HVESPFQYVSALRTAIKNAEVKVTDLAGRPYRNREVRKRADELEGLNFLPDAIEQPVR
                     VSVVITSVENCSTDRLDLVFTVFTTQVAPLLSRTFESYQLEKFAPERSAGIDAGNNPW
                     RFSPSIAYDRAEQLSGGARLEYRNANSVSSNFPFGSVIIEGRGSARMHDFSAALSGSV
                     VRGADWIEWAEWQLNYRNASNPTEESSLRQNRLVAQLTATTRPLGSVQLPLRFGGQLE
                     GGTLRSHFADADLARNTIEGSSYGALKFYLGTTMRLDRHAFAFSYGLELGSTRPGAEI
                     GWLKHIADVAHDVSFPVGDHRSLEIESRLTAGIIQVPGSIPAAARFYGGNREEPFMPG
                     DNWNIRSNPVIRSIPADHFSQTDNGRGGTRFISYNMTAGIPIWRRPLVPPELSHDSEF
                     TDELNGQLQTVTSLLQPDFAAKDPHFQRVAAQLDEVQSALDKLLAIVSTAQPSAPEPV
                     AQQFKACSSALNTAKRRVQAAAQSKGAAQYGDVSTLLTVNETEDRLNKVRSACLEGLN
                     SQLQDVQIAAAADSLEEIHSRMEKEFAQIDQSAASKQAESEMSYGKRTLNSILYEMNF
                     LSLGPILLFDVARMSPAGSKLGTRYGIGGGLRFTLISHVDFTIGYAANPKRITGQPPG
                     AFFFSMRFKDLLN"
     gene            2793..3767
                     /locus_tag="VFY96_01675"
     CDS             2793..3767
                     /locus_tag="VFY96_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX5605191.1"
                     /translation="MITSKDLKGIMAMMPAFTKKDGDRIDATDTVDTDELTRAVDRLI
                     RDGGVNVLTTTGSFGEFHTLLWDEHKKLIEATVAAVNKRVPVFVGCTNLNPREAIRQA
                     KFAQQAGADGLLLGVPFYYQATVDNAVQFYHDVADALPNMGVMIYHNPTHHRVTIPVG
                     AFKKITEKPNIVGMKDSHRTPLQFVELCALTKGKLSVFVNQTQMFPYYQLGAAGCWSF
                     NVWMGPSPVVNLVDACLAQDWETAKRICLDLDATNKVGPNIGNLSWRENVFKLAVNEA
                     GYCSAGPLRAPWRIVPQEVIDNSKKIAAYWKTLCEKYPLAEQQVRKTA"
     gene            3895..>4751
                     /locus_tag="VFY96_01680"
     CDS             3895..>4751
                     /locus_tag="VFY96_01680"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5605192.1"
                     /translation="MDEQKHKIIDGDGHVVEDFASIIKYMPEEYVGRSFSEARGKNPF
                     PPIDHLHSANRHYTPKGAFANVGREGWELFLEDVGIGTTVLYTSAGLAFGKIVSRDWA
                     IELARAYNNWMYDTYTSKSTRFKAMGLVPLQEPAEAVIELRRIVRDLGFCGAMLPSTG
                     VNMPQLGAQKYWPIYGEAERLGCALAIHGGAHENLLMDDMSPYAVANALGHPMSQMIC
                     FAGIVFNGIFDKFPGLRIGFMEAGCAWLLTCMERFTGSWASHVQYDPRGRFLQLKKGE
                     KIIDYIVRH"
BASE COUNT         1095 a         1354 c         1314 g          988 t
ORIGIN      
        1 ctatgacgtg gattttttca ctgctggcag agactattcc cgagatacta tgagccgggc
       61 cggctggttc cagctccatg gttgggacca acaaaaattc ccttctcgct caaaatcagt
      121 tcctaggacg ggaaacaaac cgcgccagcg gattgctcgc agccattcaa aacgaggagg
      181 aaagatgaga cgcagagcga aaaccagggc cgcccgcgat ctgttcgcac ccgcgagcga
      241 gccgaagcga accatcgttg gaataaaagt caagttcgaa ttgaactcga aaaacggcct
      301 tcgccgcgtc attttcggac tggcgaaaag cagtcagggc gatgatgttc tgtggaaaat
      361 taatttcgcg ctcttcgagc gcgccaagaa gtccgatcct tatggcgacg cactcctcga
      421 tatggacgtc gaagtcgaca cccagctaag caagaaagcc gaagataccg cgcagaaggg
      481 tttgactccg ggccaggcag cccatgccct cggaccggcg gcagaggacg ccaaagcggc
      541 agcggccggc gaaatcgatc atgaggaagc gagtgacacc gtacaggcga ccctgagaaa
      601 aaaatagccg ctctccgccc aatggcgtat gttctccttc taaccttcgc aatcatcctg
      661 tcatcgcgag ttcaggccgc cagcgttctc tctgcgggca attgtcccga cggagtcgat
      721 ttcgaaaaat ccggatactc gattcgcttc gctcatgtcg aaagcccatt tcagtacgta
      781 agcgcgctgc gcactgcgat caaaaacgct gaagtaaaag tgacagactt agccggtcgg
      841 ccgtatcgaa atcgcgaggt gcgcaaacga gccgacgagc tcgagggact gaatttcctt
      901 ccggatgcaa tcgaacagcc ggtgcgggtt tcggtggtga tcacgtcagt ggaaaactgc
      961 tccaccgatc gactcgatct tgtttttact gtcttcacga cgcaggttgc tccgttactg
     1021 agtcgcacct tcgaatctta tcagctcgag aaattcgcgc cggaacgaag cgccggaatt
     1081 gacgcaggaa ataatccgtg gcgcttttct ccgtcgattg cctacgaccg cgccgaacag
     1141 ttgtccggcg gcgctcgctt ggaatatcgc aatgccaatt cggtctcgag taactttcct
     1201 ttcggttccg tcatcatcga aggacgcggc tcggcgcgaa tgcacgactt cagcgcggcc
     1261 ctgagcggct cggtcgttcg cggcgccgac tggatcgaat gggccgagtg gcaactgaac
     1321 taccggaacg cgtcgaatcc taccgaagag tcgagcttgc gtcagaatcg tttagtcgcg
     1381 cagttgaccg cgacgacgcg gccgcttgga tctgtgcagt tgccgctccg tttcggcggc
     1441 caattggaag gaggaaccct gcgcagtcac tttgccgatg ccgatctcgc gcgcaacacg
     1501 atcgagggtt ccagctacgg cgcgctcaag ttctatcttg ggacgacaat gcgattggac
     1561 cgccatgcgt ttgccttctc ctatggtctg gagttgggtt cgacgcggcc aggcgccgaa
     1621 atcggctggc tcaagcacat cgccgatgtc gcacacgacg tctcctttcc tgtcggcgac
     1681 catcgttccc tcgagatcga atcgaggctg acggcgggca tcattcaagt gcccggctcg
     1741 atcccggccg ctgcgcggtt ttatggcggc aaccgggagg agccgttcat gcccggtgac
     1801 aactggaaca tccgcagcaa tccggtcatt cgtagtattc cagccgatca cttttctcag
     1861 actgacaacg gccggggtgg cacacgattc atcagctaca acatgactgc tggaatacca
     1921 atttggcgcc gtcccctcgt tccgcctgaa ctgtctcacg acagcgagtt tacggatgag
     1981 ctaaacggtc aactgcagac tgtaacgagc cttttacaac ccgattttgc ggccaaggat
     2041 ccgcacttcc agcgtgtcgc ggcgcaactc gatgaggtgc aaagcgccct ggacaagctt
     2101 ctggcaatcg tgagcactgc ccagccatcg gctcccgagc cggttgcgca gcagttcaag
     2161 gcctgttcga gcgccctcaa taccgcaaaa cgccgcgtgc aagctgcggc gcagtccaaa
     2221 ggcgccgcac agtatggcga cgtttcgacc ttgcttaccg tgaacgaaac tgaggaccgc
     2281 ctgaacaaag ttcgcagcgc gtgtctcgaa ggcttaaata gccaactcca agatgtgcaa
     2341 attgccgcgg ccgcggacag cctggaagaa attcattcgc ggatggaaaa agagtttgct
     2401 caaatcgatc aaagcgcggc ttcaaaacag gccgaatccg aaatgagcta cggcaaacgc
     2461 actttgaaca gcattctcta tgaaatgaat tttctgtccc tcggaccgat cctactgttc
     2521 gacgtcgcgc gtatgagtcc ggccggttca aaactcggca cgcgctacgg catcggtggc
     2581 gggctccgtt ttacgttaat cagccatgtt gatttcacca tcggctatgc cgccaacccc
     2641 aaacgcatca cgggccaacc tcccggcgcc ttcttttttt ccatgcgctt caaagatctg
     2701 ctgaactaaa acgctgtgca tgaactccag ttcgacttga ctcccgcgat ctcgtattgg
     2761 agaatccggc aaatctcagc gaggaggctt gaatgattac ttcgaaggat ctcaaaggca
     2821 tcatggcgat gatgccggcg tttacgaaaa aagacggcga ccggatcgac gccaccgata
     2881 ccgtggacac cgacgagctg actcgcgccg tggataggct gatccgagat ggcggcgtga
     2941 atgtgcttac cacgaccggc agtttcggtg aatttcacac gctactttgg gacgagcaca
     3001 aaaaactcat cgaagcgacc gtggccgcag taaacaaacg cgtcccggtg tttgtcggct
     3061 gtacgaatct taatccgcgt gaggcgattc gccaggccaa atttgcccag caggcgggtg
     3121 ccgacgggtt gttgctcggc gtgcctttct actatcaggc caccgtggac aacgccgtgc
     3181 aattttatca cgacgtcgcc gatgccctgc cgaacatggg cgtgatgatc taccacaatc
     3241 cgacgcatca tcgcgtcacg attccggtcg gcgcgttcaa aaagatcacc gagaagccaa
     3301 acatcgtggg gatgaaagac tcccatcgga cgccgctcca gttcgttgaa ttgtgcgctc
     3361 tcaccaaggg aaagctgagc gttttcgtca accagacgca gatgtttcct tactaccagc
     3421 tcggcgccgc cggctgctgg tccttcaatg tctggatggg gccgtcgccg gtggtgaatt
     3481 tggtcgatgc ctgcctcgcg caagactggg aaacggcgaa acggatctgt ctcgatctgg
     3541 acgcaacgaa caaagtcggg ccgaatatcg gtaacctctc gtggcgcgag aacgtcttca
     3601 aattggccgt caacgaagca ggctactgca gcgcgggtcc tttgcgcgct ccctggcgaa
     3661 ttgttccgca ggaagtcata gacaacagca aaaagattgc tgcgtactgg aagacactgt
     3721 gcgaaaaata tccgctcgca gaacagcaag tgagaaaaac cgcatagcaa gagtctagag
     3781 tctacggtct aacgtcttcg gttccatccg aacccaaact cgagacacca aacgctggac
     3841 ttggttaatc gaagcggacg cgttacacgt taagcgtata ccaggagaaa gctcatggat
     3901 gaacaaaagc ataaaatcat cgacggcgac ggacatgtgg tggaagactt cgcgagcatc
     3961 atcaaataca tgcccgaaga gtatgtcggt agaagctttt ccgaagcgcg cggcaaaaat
     4021 ccgttcccgc ccattgatca tttacattct gccaatcgcc actacacgcc gaagggcgcc
     4081 ttcgccaatg tcgggcgcga gggttgggaa ctcttcctcg aagacgtcgg catcggcacg
     4141 accgtgcttt ataccagcgc gggtctggca ttcggcaaga tcgtcagccg cgactgggcg
     4201 atcgaattag cgcgggcgta caataactgg atgtatgata cctacacatc gaaaagcaca
     4261 cgcttcaagg cgatgggctt ggtccctctt caagaacctg ctgaagcggt tatcgaactg
     4321 cggcgcattg tgcgcgatct cggattttgc ggcgccatgc tgccgtccac cggcgtcaac
     4381 atgccgcagt tgggcgcgca gaagtattgg cccatttacg gcgaggcgga acggctcggt
     4441 tgcgcgttag cgatccatgg tggcgctcat gagaatctct tgatggatga catgagtccc
     4501 tatgctgtgg ccaatgctct cggccatccg atgagccaga tgatttgttt cgccgggatt
     4561 gtgtttaacg gcatcttcga caaattcccc ggcctgcgca tcggtttcat ggaagccggt
     4621 tgcgcctggc ttctgacctg catggagcgc ttcaccgggt cgtgggcaag ccacgtgcaa
     4681 tacgatccgc gcggtcgttt cctgcaactc aagaagggcg aaaaaattat cgactacatt
     4741 gttcggcata t
//
LOCUS       DASULZ010000083         2498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_415065, whole genome shotgun sequence.
ACCESSION   DASULZ010000083 DASULZ010000000
VERSION     DASULZ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415065"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..390)
                     /locus_tag="VFY96_01685"
                     /pseudo
     CDS             complement(103..390)
                     /locus_tag="VFY96_01685"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            complement(608..>2498)
                     /locus_tag="VFY96_01690"
     CDS             complement(608..>2498)
                     /locus_tag="VFY96_01690"
                     /inference="COORDINATES: protein motif:HMM:NF042664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HEX5605193.1"
                     /translation="GLTGGTTGQFIQQGNIRTDMFEQLLIADLVIADISIHNANVFYE
                     LGIRHALRDKRTFLIKSRDSKPQGVETEVPFDLKTDRYLSYDARNPGRAVDILAAALR
                     LTLDAQNPDSPVYHLLPGLKPADPTKVLVVPFDFREEVERAEAGSNCGNLQLLASEVD
                     GFAWRIAGLRLIGNAQFRLKDWQGAKVTWLGVREYDESDVEANTFLATIFQRLGDLVS
                     SDQAVERALQNKDISPLQRAEIRALMGRNAKNQWEQSWAKPNGTEATQQAALTSPYLE
                     KSLDLYRAGFSEDRNHFYSGLNALAMATMLIELAQCQSNIWQDNFDSEEEANQKLAKL
                     KELRSDLTAAVKLAIQSKQTALERTGKSDVWTDISTADLTCLTSGRPNRVAQAYKKAL
                     ANATPQARDAVRRQLLLYQRLGILNENIKAGLEGIGPVAAQAQELPPRVILFTGHRID
                     TPDRASPRFPAAKESLARDMIRDAVVREIARGSERIIGVAGGASGGDILFHEICDELK
                     IPSQMYLVWPKNEYIKASVADGGSEWVERFNRLFDKHEPKILSESERLPRWLRAKADY
                     DIWQRSNLWMLHNALFLSREHLTLIALWNGATGDGPGGTEDMVDRAKNRGASFIHLDA
                     RKLLD"
BASE COUNT          546 a          707 c          662 g          583 t
ORIGIN      
        1 tggctattcg tcggtgacct ccgtattcct gccgttttgg atcggcaatg aagccggttt
       61 ttacaatcgg gcagcttctg acgtaatggc tcggctgatg gttcaacaat ggagctgctg
      121 cggaagtttt tcaaactttt catccaaccg ttccatcaag tagggaaccc agccgacccc
      181 ggcttctaaa aacgccactt tgagccgcgg aaatcgcacc ggcgcgccat cgagcaaata
      241 gctcgtgaaa tggatttgct gcgcgaccgc gtgttccatc accaggattt ttacgaagga
      301 atcgtacagg tcgaggccga gaccggcgct cgaaccgccg tgcacggcca atggcatatt
      361 aagccgcacg gcagccttat accccggata tcgaggcctt ctttcttgta aaaaccggct
      421 tcattgccga tccaaaacgg caggaatacg gaggtcaccg acgaatagcc aacgacgagc
      481 ggcgcggcgc atgccccgac ggcaaataac agggacaagg cgacgcccag aaagaggctt
      541 ttcatgaagc cgatgagctg cgcgagggtt gtcatttcga ccgaagggag aaatctttcc
      601 aaaagtctta atcgagcaat ttgcgcgcat caagatggat aaagctcgcg cctcggttct
      661 tcgctcgatc caccatatct tcggttccgc ccgggccgtc ccctgtggca ccgttccaga
      721 gcgcgatcaa cgtcaagtgc tcacgggaca aaaacagcgc gttgtgcagc atccataaat
      781 ttgagcgctg ccagatgtca taatccgcct tcgctcggag ccaacgcggc agccgctccg
      841 attcggacaa aatcttgggc tcgtgtttgt caaaaagccg attgaatcgc tccacccact
      901 ctgaaccccc atcggcaacc gaggctttga tatattcatt cttcggccat accaaataca
      961 tctggcttgg aattttgagt tcgtcgcaga tctcgtgaaa aagaatatcg ccgccggaag
     1021 cgccgccggc gacaccgatg atccgttcgc tgcctctcgc gatttcccgc accaccgcat
     1081 cacggatcat gtctcgcgct aagctctcct tggccgcagg aaaacgaggg ctggcgcgat
     1141 caggggtgtc gatccgatgg ccagtaaaca aaataacccg gggcggcagt tcctgagctt
     1201 gcgccgccac ggggccaatt ccttccagac cagctttgat attctcgttg aggattccca
     1261 agcgttggta cagtaaaagc tgtcgtcgta ccgcgtcgcg cgcctgcggc gtggcgttgg
     1321 cgagcgcctt tttgtaggct tgagcgacac gattgggtcg gccggatgtc aaacaagtta
     1381 gatccgcggt actaatgtcg gtccacacat cgcttttgcc agtgcgttcc agggcagtct
     1441 gtttcgattg gatcgccaac ttgacggcgg cggtaagatc gctgcgcagt tccttgagct
     1501 tagccaactt ttgattggct tcttcttccg agtcaaagtt gtcttgccaa atattacttt
     1561 ggcattgcgc caattcgatg agcatcgtcg ccatcgccaa tgcattcaga cccgaataga
     1621 agtggtttcg atcctcgcta aaacctgcgc ggtaaagatc cagtgatttt tctaaatacg
     1681 gcgaggtcag agccgcctgc tgcgtggcct cagtgccgtt tggttttgcc cagctttgtt
     1741 cccattggtt cttggcgttt cggcccatca aggcgcgaat ttcggcgcgt tgaagaggag
     1801 aaatgtcttt attctgaagc gcccgctcca ctgcttgatc cgagcttacg agatcgccca
     1861 agcgctggaa gatcgtcgcg agaaatgtat tggcctcgac atcactttca tcgtactctc
     1921 gcactccaag ccaggtcact ttcgcgccct gccaatcctt caatcgaaat tgcgcattac
     1981 cgatgagccg aaggccggca atgcgccaag caaagccatc cacctcgctg gctaaaagct
     2041 gtagattgcc gcagttgctt ccggcttctg cccgttcgac ttcctcgcga aaatcgaacg
     2101 gaaccaccaa gactttggtt gggtcggccg gttttagccc cggtaataaa tggtagaccg
     2161 gactgtcggg attctgtgca tccaaagtaa gtctgagcgc ggcggccaat atgtctacgg
     2221 ccctgcccgg attgcgcgca tcatacgaca gataccgatc tgtctttaga tcgaacggca
     2281 cctcggtttc cacgccttgc ggtttgctgt cccgggattt aatgagaaat gtccgtttgt
     2341 cgcggagcgc atgacggatg ccgagttcat aaaaaacgtt ggcgttatgg atcgatatgt
     2401 ccgcgatgac aagatcggca atcagcaatt gctcgaacat atcggtccgg atgtttccct
     2461 gctggatgaa ctgtccggta gtgccgccgg tcaggccc
//
LOCUS       DASULZ010000084         3579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_719394, whole genome shotgun sequence.
ACCESSION   DASULZ010000084 DASULZ010000000
VERSION     DASULZ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719394"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..465
                     /locus_tag="VFY96_01695"
     CDS             88..465
                     /locus_tag="VFY96_01695"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX5605194.1"
                     /translation="MPFRFSIPQRWLIYLFSALIVLLALITVVGERGVLHLWRLRGEK
                     NRLDEQNFRLQKENEALRQRISRIRNDNAYLEKLAREELNLVRPGEIVYRFSNSAARP
                     ADQKPDSRPGTKPRGAPGPKPKP"
     gene            604..1116
                     /locus_tag="VFY96_01700"
     CDS             604..1116
                     /locus_tag="VFY96_01700"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5605195.1"
                     /translation="MDTRDPKAAATLILSGLKKAGINFVATLPDEKMLELIRAVEKDP
                     DLKHVPLCREEEGIGICAGAYLAGKKPAIIMQNAGFLNSCNALTTTSLQFQIPILMLI
                     YYAGDIGDRGFTTLGAVTEPVLEAMGFRSYVLRRTDEVDEILRGAQILAEDAKTPVAV
                     LLTKTILGVN"
     gene            1116..1715
                     /locus_tag="VFY96_01705"
     CDS             1116..1715
                     /locus_tag="VFY96_01705"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5605196.1"
                     /translation="MQRYDYLKAIVPHAEDPLAVCTGWAAREWWALRPSEGNLKTRTL
                     GLVSSIAAGLAIALPHRKVIAIDGDGAFLMNLCGLPTIALQKPGNLIHLLFDNEIYEA
                     SGGTATASRNSDAVALALGAGYKHALWVSDPGEFATEFTAACKRGELTLIAAKVDPGQ
                     PKGLPSLKMDEIENKYRFMRYLEATENKPLLSPGFDSKL"
     gene            complement(1751..2740)
                     /locus_tag="VFY96_01710"
     CDS             complement(1751..2740)
                     /locus_tag="VFY96_01710"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5605197.1"
                     /translation="MRIKKGRSTIAEGFYNFRYRCGEFAVRGFMATLPYLPPPVVSGF
                     TKSLAWLTFGLLWKYRKRMAENVASVLGSELGCDQQKVLVWRAWLNFARGVLETMTVM
                     HLSRNEIIGKIKLEGEEHLKRALEMQKGVLAISAHLGSFTLIGGRLAAAGYPFSVIVK
                     QPADKRFAQLIDDYRAQLGIHTISAKPRREAAREILKALRNNRIVLIIADEFKSGDVM
                     VDFFQMKAPAPRGPATLALRTGAVTLPMFAVRGAGESIVLTVGPAIPAEERGDIEENV
                     VATTALYTRHIEAAIRKHPDQWNWLGLPRRDGKMSRSEMARLAKGTRAVADEG"
     gene            complement(2842..3300)
                     /locus_tag="VFY96_01715"
     CDS             complement(2842..3300)
                     /locus_tag="VFY96_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605198.1"
                     /translation="MSYDTGLSMEERVTSLFQPDTLLPDQYLDTFRRKLYVEPEKKLM
                     LAILDDAIACFQKYAFARDSKGKALFTEAEEWIQQGAAGGIFSFDSVCEVLGLNPSYL
                     RQGLGAWKENALAARSPAKVYRLSQSATKQKQPARVSRMAQRRLRRAAGR"
     gene            complement(3364..>3579)
                     /locus_tag="VFY96_01720"
     CDS             complement(3364..>3579)
                     /locus_tag="VFY96_01720"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX5605199.1"
                     /translation="DVIEAIEGPIAVNACMDHALGCDQIPRCRMLGVWSEVQRSVTEV
                     LSRTTIAALRQPACGDLVHSSSLSSAA"
BASE COUNT          813 a         1051 c          916 g          799 t
ORIGIN      
        1 ccgttgaatt ctcatcgttg tcctccaaga aactaagtca ggtaagacaa aagcataaat
       61 tgtgatatat tgctatgtga cttgacgatg ccgtttcgct tttccatacc tcagcgctgg
      121 ttgatctatc tctttagcgc cctaatcgtt cttctcgcat tgattactgt cgtcggcgag
      181 cgcggagttc tacacctatg gcggcttcgt ggtgaaaaga atcgcctgga tgagcaaaat
      241 tttcgtttac aaaaggaaaa cgaagctttg cgccaacgca tttcccgcat ccgtaatgac
      301 aacgcgtatc tggaaaagct cgcgcgcgaa gagcttaatc tcgtgcggcc cggcgaaatc
      361 gtctatcgct tttccaattc ggcagctcga cctgccgatc aaaaaccaga ttcgcggcct
      421 ggcacaaaac cgcgcggcgc acctggacca aagcccaaac cgtgatttcg ttgcgagact
      481 ccagtgaaag gcaaccgcgc tccgcggctt gacaacctcg actcgttcac agtaccggtc
      541 aatacggcac cgtccgcgtt gtgaaagcgg acacgcctag ttctcgtcga ggcacattaa
      601 accatggaca ctcgagatcc gaaggccgca gccaccttaa ttctcagcgg gctgaaaaaa
      661 gccggcataa attttgtcgc tactctgccg gatgaaaaaa tgctcgagct tatccgagca
      721 gtggaaaaag atccggattt gaagcacgtt ccactctgcc gtgaagaaga aggaatcggc
      781 atctgcgcgg gcgcatatct tgccggcaag aaaccggcga ttatcatgca aaacgccggc
      841 ttcttgaaca gctgcaacgc tctgacgacg acttcgctgc agtttcaaat tccaattctg
      901 atgttgatct actatgccgg cgacattggc gaccgcggct tcaccaccct cggggccgtc
      961 accgaaccgg tgctcgaagc gatgggcttt cggagttacg tattgcgccg cacggatgaa
     1021 gtcgatgaaa tcttgcgcgg cgctcaaatc ctggcagaag atgctaagac accggtagcc
     1081 gtccttttga cgaaaaccat actcggagtg aattgatgca gcggtacgac tacttgaaag
     1141 cgatcgtgcc ccatgccgag gaccctctgg cggtttgcac cggctgggct gctcgagagt
     1201 ggtgggcgct ccggccgagc gagggcaatt taaaaacccg cacccttgga ttggtctcat
     1261 cgattgccgc cgggttggcc atcgctctac cgcaccggaa ggtgatcgcg atagatggcg
     1321 acggcgcgtt tctcatgaac ctatgcgggc tgccgacgat cgcattgcaa aagcctggca
     1381 atctgattca tctgcttttc gacaatgaga tctatgaagc atcagggggt acagccacgg
     1441 cttcgcgcaa cagcgatgcg gtggcgcttg ctttaggagc agggtacaag cacgcacttt
     1501 gggtaagtga tccgggagag tttgcgacgg aattcaccgc cgcctgcaaa cgaggtgaat
     1561 tgaccttgat cgctgccaaa gtagaccccg ggcaacccaa aggtcttccg tctctaaaga
     1621 tggacgagat cgagaacaag taccggttta tgcgttatct cgaagcgacc gaaaataaac
     1681 cgctcttgag cccaggtttc gattcaaagc tctgaaccgg ttcaatcgtc ccgtccctcg
     1741 gcggattttc ttagccttca tcagcaacag ctctcgttcc ctttgcgagc cgggccattt
     1801 cagagcgcga cattttaccg tcccggcgtg gtagaccgag ccaattccac tgatcgggat
     1861 gtttccggat cgccgcttcg atgtgacgag tgtagagcgc cgtggtcgcc acgacattct
     1921 cttcgatatc gccgcgttct tctgcaggaa tcgctggccc aacggtcagc acaatagact
     1981 cacccgcccc tcttaccgca aacatcggca aggtcaccgc gcccgtccgt agcgctaacg
     2041 ttgccggacc acggggagca ggcgccttca tttggaaaaa atccaccatg acgtcacccg
     2101 atttgaactc atccgcaata atgagaacaa tccgattgtt gcgtagcgcc tttaaaattt
     2161 cccgcgcagc ctcccggcgc ggcttcgccg atatcgtgtg aatgcccagc tgggcgcgat
     2221 aatcgtcgat cagttgggca aagcgtttat cggccggctg ttttacgatc acgctgaacg
     2281 ggtagccggc ggccgccagc cgcccgccaa tcagagtgaa acttcccaga tgcgcgctta
     2341 tcgcaagcac acccttctgc atctctagcg cgcgtttgag atgctcctct ccttccaatt
     2401 tgattttgcc gataatttcg ttcctcgaca aatgcatgac cgtcatggtt tcgagcacgc
     2461 cgcgggcaaa attcaaccaa gctcgccaaa ccaagacttt ttgctgatca cagccgagct
     2521 ctgaacccag gacggatgcg acattttcgg ccattctctt gcggtatttc cacaagagac
     2581 cgaacgtcag ccaagccaag gacttcgtga atcccgagac tacaggtgga ggcagataag
     2641 gcagtgtggc catgaatccg cggaccgcga attcaccgca tcgatacctg aaattgtaaa
     2701 acccttctgc aatagtcgac ctgccctttt ttattcgcat gccctatctt tgaatttgtt
     2761 tacgaactat ttcaagcaaa taaaaagacg gcgacggaat tctccgtcgc cgtccccttc
     2821 gaactcggtt agctgagccg gttaccggcc cgccgcccgg cgcagacgcc gctgagccat
     2881 gcgtgatact cgagctggct gcttttgctt tgtcgcgctt tgagacagac ggtaaacctt
     2941 cgctgggctc cgcgccgcca aggcgttctc cttccacgcc ccgaggccct gcctcaggta
     3001 actgggattt aacccgagca cctcgcacac gctgtcaaac gaaaaaatgc ctccggccgc
     3061 gccctgttga atccattctt cggcttcggt gaataacgct ttccctttgc tgtcgcgagc
     3121 gaaagcatat ttttggaaac acgcgatagc atcgtccaga atcgccagca tcagcttttt
     3181 ttccggctcc acatacaatt tgcgcctgaa cgtatccagg tactggtccg gcaaaagcgt
     3241 gtccggttga aacagcgacg tcacgcgctc ttccatacta agtcctgtat cgtaactcat
     3301 agaaccacct cccgttggag tacggcttgg gtgcaagtga attcatggca atcttcaccc
     3361 ggctcacgcc gcactcgaaa gagaagagga atggactaaa tctccacagg ccggctgccg
     3421 tagagccgcg atcgttgtgc ggctgaggac ctcggtgacg cttcgttgca cctcgctcca
     3481 cactccgagc atccggcagc gcgggatctg atcgcagccc aacgcgtgat ccatgcaggc
     3541 attgaccgcg ataggcccct cgatggcctc gatcacgtc
//
LOCUS       DASULZ010000085         2949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1328578, whole genome shotgun sequence.
ACCESSION   DASULZ010000085 DASULZ010000000
VERSION     DASULZ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328578"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..1425)
                     /locus_tag="VFY96_01725"
     CDS             complement(133..1425)
                     /locus_tag="VFY96_01725"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5605200.1"
                     /translation="MLMINIGCAKNSSMNQSGNITEETSRLSLFRHREFCVFWTGSFL
                     SSVGTQFTTVAMAWQIYELTNSPLQIGLLGVARALPQIALLLVGGLLADAVDRRKLMM
                     CTQMGLFCVSTTLAWLTLAGKASPQMLYIATVFLAFFTSLEQPSRQSMIPNLVPRPLL
                     AQALALQGTQRYVPIIAGPSIAGVVLAVWGPAACYSVDACSWLAMLAALVLLRAKIPQ
                     GGGWKNISVASLHEGLQFVWSHGVIFPLMVLDFSATFFGNVRGLFPIYARDILFVGPK
                     GLGILYAARAVGSLIAAASMALFGPVRHDGRWIFLGIGIYGISTVLFSGSQVFWFSVL
                     MLTLTGAGDTISSILRSTINQLSTPDELRGRMSSINSIFTSSGPQLGQFESGLVAAWL
                     GAQGSALTGGLATLFVLAIVAGTFPRVRRFQNPHFSIS"
     gene            1601..2620
                     /locus_tag="VFY96_01730"
     CDS             1601..2620
                     /locus_tag="VFY96_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007229116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HEX5605201.1"
                     /translation="MRNGKIRVGIMGLGQIGRHLYHLALENDDIEIVAVADIGKPEII
                     EYLLKSEIKDELQCQLKDNYLVNGKFRTRMFQHARPEEVPWDVMNIDCVVDATGRYRR
                     RQDMEGHLRAGAKRVILASLPADPIDRIVIPGINEKQASLSDRIISAGSATTNAFALL
                     LQSLDSALGVDCASMTTVHAYSSDQSLQDYAHEDFRRSRSAAENIIPNKNESAHWVET
                     ILPKFAGKLSGYALNVPVQRGSLLDVNCVMQNSGVTVDQVNEAMRSAAARDPELIGVS
                     DDPIVSSDVIGCRQSLLFDSRATIQAGKKIIKTLAWYESLGHACRVLEVVRQYGKLGT
                     TGGER"
     gene            2617..>2949
                     /locus_tag="VFY96_01735"
     CDS             2617..>2949
                     /locus_tag="VFY96_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008294828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HEX5605202.1"
                     /translation="MRVAINGFGRIGRSVFRILDKRADADLVAINDVFDHDALRYLLN
                     YDTVMGRFEGVLRLEGDELVTSHGRTRMIQERDPLKLPWRALGIDVVIESTGIFTARE
                     QLQKHLEAG"
BASE COUNT          714 a          819 c          849 g          567 t
ORIGIN      
        1 agccttgctc ctgggcaacg gcgatcgcaa tctggtgacc gacgtgataa ttgctgttgg
       61 caatcagaat aggctggtca agtgtgctca tggcgactcc agtcgattaa agttccatcc
      121 gattcccgac gttcagctga tcgaaaaatg aggattctgg aaccggcgca ccctcggaaa
      181 agttcccgcg acaatcgcaa gcacaaaaag ggtggccaat ccccccgtca aagccgatcc
      241 ctgagcgccc agccaagcag cgaccagacc cgattcaaac tggccgagct gcgggccgct
      301 actggtgaaa atactgttaa tcgacgacat ccgcccgcgc aattcgtccg gtgtgctgag
      361 ctggttgatg gttgagcgca ggattgagct gatggtatcg ccggctccgg tcaacgtaag
      421 catcagaacc gaaaaccaga acacctgcga gccggagaac agcacggtcg agattccata
      481 aatgccgatg ccgaggaaga tccaccgtcc gtcgtgccga accgggccga acagcgccat
      541 gctcgcggcc gcaatcaaag agccgaccgc gcgcgcggcg tataggattc cgagtccttt
      601 gggtccgacg aacaaaatgt cgcgcgcata gatcggaaac aatccgcgca cattgccaaa
      661 aaaggtcgcg ctgaagtcca acaccatcag gggaaaaatg actccgtgcg accagacgaa
      721 ttgcaagcct tcatgcagcg acgccactga aatatttttc cagcctcccc cctggggaat
      781 tttggcgcgg aggagaacga gcgctgccag catggccagc cacgagcagg catcgacgga
      841 ataacaggcc gcgggccccc acactgcgag aaccacgcca gcgatagaag gtccagcgat
      901 gatcggcacg tagcgctgtg tgccttgcaa tgccaaagct tgcgcgagca atggccgcgg
      961 caccaggttc ggaatcatcg attgacgcga cggctgctcc agggatgtaa aaaatgccaa
     1021 gaacactgtg gcgatgtaga gcatctgcgg gctggctttc ccggcgaggg ttaaccaagc
     1081 gagagttgtg gaaacgcaaa acaagcccat ttgggtgcac atcatcaatt tgcgacggtc
     1141 caccgcatca gccagtaatc caccgaccag tagcagcgcg atttgcggca gcgcacgagc
     1201 gactccgagt agaccgatct gcagcggcga atttgtcagc tcatagatct gccaggccat
     1261 tgcgacggtg gtgaattgag ttccaacgct cgatagaaaa gaacccgtcc agaaaacgca
     1321 aaactcacgg tggcggaaaa gcgacaaacg gctggtctct tcggtgatat ttccgctctg
     1381 gttcatcgaa ctatttttcg cgcagccaat gttgatcatc aacatgcgcc gccgcgattg
     1441 ccgtgtctta tctcaaagcc agcggaacga caaaccgagc cgcgacagtc actgcaaaaa
     1501 tttcagcaag gcgtcggaaa aatctgtagg tttcatcaaa gctcaggtat aatattgcca
     1561 ttccactaac acgacttctt cagaaccgga ggtaagccaa atgcgcaacg gaaagatccg
     1621 tgtcggcatt atgggcttgg ggcagatcgg tcggcacctc taccacctcg cgctggaaaa
     1681 tgacgacatc gagatcgttg ccgtagccga tattggcaag cccgaaatta tcgagtatct
     1741 gctcaaaagc gaaattaaag acgagctgca gtgccagctc aaagacaatt atctggtaaa
     1801 cggcaagttt cgtacgcgga tgttccagca cgcgcggccg gaggaagtgc cgtgggatgt
     1861 tatgaatatc gattgcgtgg tggatgcgac cgggcgatat cgtcgacgcc aggatatgga
     1921 aggccacctg cgcgccgggg cgaaacgtgt gattctcgca tcccttccag cggacccgat
     1981 cgatcgaatc gtgatcccgg gaatcaatga gaagcaggca agcctcagcg accggatcat
     2041 ctcggcaggt tccgcgacca ccaatgcgtt tgcgttgttg ctgcagtccc tagactccgc
     2101 gctgggggtg gattgcgcat cgatgaccac ggtgcacgcc tactccagcg atcagtcgct
     2161 gcaggattac gcgcatgaag attttcgtcg cagccgctcc gccgccgaaa acatcattcc
     2221 caataaaaac gagtcggcgc attgggtcga aactattctg cccaagtttg ccggaaagtt
     2281 gagcggttat gcgctgaacg tgccggtgca gcgcggctcg ttgctcgatg tgaattgcgt
     2341 catgcagaac agcggcgtca ctgtcgatca agtcaacgag gcgatgcgtt cggccgcggc
     2401 gcgcgatccg gaactgatcg gcgtcagcga cgatccgatc gtatcttccg acgtgattgg
     2461 ttgccgacag tctttgctct tcgactcgcg cgcgacgatc caagccggta aaaaaatcat
     2521 caaaacgctc gcctggtacg agagtttggg gcacgcctgc cgcgtgctcg aggtcgtgcg
     2581 gcaatatggc aagctcggca ctaccggagg tgagcgatga gagttgcgat caatggtttt
     2641 ggacggatcg gccggtcggt ttttcggatt ttggacaaac gcgcggatgc cgatctggtc
     2701 gcgatcaatg acgttttcga tcatgacgcg ctgcgctatc tgcttaacta tgacacggtg
     2761 atggggcgat tcgagggtgt cttacggctt gaaggcgacg agcttgtgac ctcccacgga
     2821 cgcacgcgca tgatccaaga gcgagatccg ctgaagctgc cgtggcgcgc tctgggaatc
     2881 gacgtcgtga tcgaatcgac gggcatattc accgctcgag aacagctcca aaagcatctc
     2941 gaagccggc
//
LOCUS       DASULZ010000086         3174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_110997, whole genome shotgun sequence.
ACCESSION   DASULZ010000086 DASULZ010000000
VERSION     DASULZ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110997"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VFY96_01740"
     CDS             <1..582
                     /locus_tag="VFY96_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017743048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5605203.1"
                     /translation="DYREGSVGSPIEDVEMKIVDPDGLELPIGEQGEIAIKGPNVMLG
                     YFRRPEETAQVMRDGWFLTGDIGKRDHDGHFYLVDRAKDMINVSGFKVWPREVEEVLL
                     QHPSVSEVGVIGVADPVSGEAVKAFAVLKQGESIRERDLIDYARGRIAVYKAPRFVEF
                     IDALPRNPAGKVLKRELRARANIPENAASMRVA"
     gene            593..1756
                     /locus_tag="VFY96_01745"
     CDS             593..1756
                     /locus_tag="VFY96_01745"
                     /inference="COORDINATES: protein motif:HMM:NF014820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEX5605204.1"
                     /translation="MSEQVAIIGIGVVPLQPTTPRYSFKEMVFEAAQKAYADAGISFD
                     EIDSFVTCAEDFNEGISIFDEYTPDQLGCRQKPMHTLTQDGLHGIADALMQIRAGVAE
                     IVVVEAHSKASNILTPDWLLDYALDPIYTRPLGFTPHALAAMDMQAFTHQTGITPSQC
                     AEVVIKNRLNARDNPLAAYALESNRTSVEASPYFSFPLHEEELARAADGCVVVVVANE
                     TTASKAAKRPAWILGTGFANDSPTIESRRWGHAEYARLAAQSAFRQAGIKTAQEIDFF
                     EVDDTYAYKELQHLIALGLYAEPIEAGRAIESGATRRDGRTPVNVSGGALGMGLPLEA
                     HGLYRIVELVLQLRGRAGDRQLSRAKIGLAQSWRGVPTTSGAVVILGAEPPRG"
     gene            1731..2924
                     /locus_tag="VFY96_01750"
     CDS             1731..2924
                     /locus_tag="VFY96_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010866978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HEX5605205.1"
                     /translation="MERNLHVAKRRVAVIGAGMTRFMHRALETPKELAWHAAHAALAS
                     SELKLNQIDCVVLGTAPDAFDGVHMKSEYLADGCGGWGKPFLRCYVGGGTGVYAVAQA
                     YFQVASGMFDSALVICEEKMSSCQPHPQAAFLTIFDPITEQPLKPNLLWIFALEQTRY
                     METYGITIEEIARVAVKNKRNALNHPAAQLGAKLTVEDVLASEIMAWPVHRLMVSPTS
                     DGAAAIVIAAEEVAPRLSDKPIWIQGIGWALDSAYWTNRDLAYPEYVEKAAWMAYDMA
                     GVKEPRKEIHIAEPYDPFAYKELHHLEGLQLAPKGEAPRLLADGVFERDGDLPVTPSG
                     GLLGVGNPIAAAGMMKICELFWQMRGEAGKRQLPGKPERGVAQAWGDLMQVGTVVVMG
                     TEKPD"
     gene            2946..>3174
                     /locus_tag="VFY96_01755"
     CDS             2946..>3174
                     /locus_tag="VFY96_01755"
                     /inference="COORDINATES: protein motif:HMM:NF023594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX5605206.1"
                     /translation="MPKFPGTPVDNRELKRGKIFMASYAPRLRYRWDTGEAIGRYLEE
                     LKRGRLIARRCDGCRRVLIPPRMFCERCFRPT"
BASE COUNT          687 a          913 c          944 g          630 t
ORIGIN      
        1 gactatcgtg agggcagcgt cgggtctccc atagaggacg tcgagatgaa aattgtcgat
       61 cccgacggac tggagctgcc gatcggcgag caaggggaaa ttgcgatcaa aggaccgaac
      121 gtgatgctgg gctactttcg gcggcccgag gaaactgcac aggtaatgcg ggacggctgg
      181 tttttgaccg gtgatattgg caagagagat catgacggcc atttctatct ggtcgatcgg
      241 gccaaggaca tgatcaatgt atcgggtttc aaggtctggc cgcgcgaagt tgaagaggtt
      301 ttgctgcagc atccgagtgt cagcgaggtg ggcgtgatcg gcgttgcaga tccggtttcc
      361 ggcgaagcgg tcaaggcttt cgctgtgctg aaacagggag agtcgatcag agaacgggac
      421 ctaatcgatt acgcgcgcgg gcggatagcg gtttacaaag caccgcgatt tgtcgaattc
      481 attgacgcgc tcccgcgcaa tcccgccggg aaagtgctca agcgagagct cagagcacgg
      541 gcaaacatac cggagaacgc agcttcaatg agagtagcct agtcgcgccg ccatgtcaga
      601 acaggtcgcc attatcggta ttggcgtcgt cccactgcaa cccaccactc cgcgctattc
      661 gttcaaagag atggtcttcg aagcggcgca aaaagcctac gcggacgccg gcatttcctt
      721 tgacgagatc gattctttcg ttacctgcgc cgaagatttt aatgaaggta tctcgatttt
      781 cgacgaatac acgcccgacc aactcggctg caggcaaaaa ccgatgcaca cgctcaccca
      841 ggatggactt cacggtatcg ccgatgcact aatgcagatt cgcgccggag tggctgagat
      901 cgtcgttgtc gaagcccata gcaaagcgtc caacatattg acacccgact ggcttctcga
      961 ttacgccttg gatccgattt atacccgacc gctcggcttc actccccacg ctttagcagc
     1021 catggacatg caggctttta ctcatcagac aggaatcact ccatcacagt gcgccgaagt
     1081 ggtcatcaag aatcggctaa acgcgcgcga taatccgctg gcggcttatg ccctcgagtc
     1141 gaatcgaaca agtgtcgagg catcgccgta tttttctttt cccttgcatg aagaagaact
     1201 agccagggct gccgacggct gtgtcgttgt ggtcgtcgca aatgaaacaa ccgccagcaa
     1261 ggctgccaag cgacctgctt ggattttggg tacaggcttc gcgaacgaca gtccgaccat
     1321 agaatcgcgc cgctggggtc atgctgaata tgcgcggctg gcagcgcaat ccgcctttcg
     1381 ccaggccggg attaaaacgg cgcaagagat cgacttcttc gaggtggacg acacctatgc
     1441 gtataaagag ctgcaacacc tgatcgccct cggcctttat gcggagccta ttgaggccgg
     1501 ccgagcgatt gaatctggcg caacccggcg cgacgggcga acgcccgtta atgtgtccgg
     1561 cggcgcacta ggcatgggtc tgccgctgga agcgcatggg ctctaccgta ttgtcgagct
     1621 ggttctgcag ctccgcggcc gggcgggcga ccgccagctg tcgcgggcga aaatcggcct
     1681 ggcgcaatcg tggcgaggcg taccgactac cagcggcgca gtcgtgattc ttggagcgga
     1741 acctccacgt ggctaaacgt cgcgtcgccg tcatcggggc gggcatgacc cgattcatgc
     1801 accgcgcgct tgagacaccc aaggagcttg cttggcatgc ggctcatgcc gctctcgctt
     1861 ccagcgaatt gaaactgaac cagatcgatt gcgtggttct cggcaccgcg ccggacgcat
     1921 ttgacggcgt ccacatgaaa agcgagtatc tcgccgacgg ctgcggcggc tggggaaagc
     1981 cttttctacg ttgttacgtc ggcggcggca ccggcgtgta tgcggtcgct caagcttact
     2041 ttcaggtcgc ctcgggaatg ttcgacagtg cgctggtgat ctgcgaagaa aaaatgtcca
     2101 gttgccaacc gcatcctcag gcggcgtttc tcactatctt cgatcccatc accgaacaac
     2161 cgttaaaacc caatctcctc tggattttcg ccctggagca gacccgttat atggagactt
     2221 acggcatcac cattgaagag atcgcccgcg ttgcggtgaa aaataagcgc aacgctctaa
     2281 accatccggc tgctcagctg ggcgcgaagc tgacggtgga agatgttctg gcttcggaaa
     2341 tcatggcctg gccggtccat cgtctgatgg ttagtccaac cagcgatggc gcggcggcca
     2401 ttgtgatcgc cgccgaggag gtcgcgccgc gacttagcga caagccgatt tggatacaag
     2461 ggatcggttg ggccctagac agcgcctatt ggaccaaccg cgatcttgcc taccccgagt
     2521 atgttgagaa ggccgcctgg atggcctacg acatggccgg ggtgaaggaa ccgcgaaaag
     2581 aaattcacat cgccgaaccg tacgatccct tcgcctacaa ggagcttcac cacctcgaag
     2641 gcctccagct tgccccaaag ggcgaagccc cgagactact ggccgacggc gtctttgagc
     2701 gggacggtga tttgcccgtc acgccttcgg gaggtttgct cggcgtcggc aaccccatcg
     2761 ccgccgccgg gatgatgaaa atctgcgagc tgttctggca aatgcgcggc gaggccggca
     2821 aacgccagct gcccggaaaa cccgagcgcg gcgtcgctca agcgtgggga gatctaatgc
     2881 aggttggcac cgtggtcgtc atgggaacgg agaaaccgga ctaaaacctc agccacaaaa
     2941 cgatcatgcc taaatttccc ggcacgccgg tggacaaccg cgaactgaag cgcggtaaaa
     3001 ttttcatggc ttcatacgcg ccacggctcc gctaccgatg ggacaccggc gaagccatcg
     3061 gtcgctatct cgaagaactg aaacgcggcc ggctgatcgc tcgccgctgc gatggctgcc
     3121 ggcgggtatt gattccgccg cgtatgtttt gcgaacgctg ctttcgcccc actg
//
LOCUS       DASULZ010000087         3411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1430200, whole genome shotgun sequence.
ACCESSION   DASULZ010000087 DASULZ010000000
VERSION     DASULZ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430200"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VFY96_01760"
     CDS             <1..191
                     /locus_tag="VFY96_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5605207.1"
                     /translation="GEFFGASKGLGFMIATAATSYKVDVVFAGVAIFMGLSLILTMAV
                     KKIESNLSRWRPEGAKTF"
     gene            complement(357..1373)
                     /locus_tag="VFY96_01765"
     CDS             complement(357..1373)
                     /locus_tag="VFY96_01765"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605208.1"
                     /translation="MNRLNLVRRRWVFCSLVAIFSTAPILLLSAYSACAQSIKIGKTI
                     GGSGFHIPSYIAMDKGLLKAEGLNADFISATASVLVRAGIAKEIEFVPIPGGGAEAML
                     KGAPLAFIVGESLVSQWAIVTQPEFKRVEELKGKTIGLERPGQAAYTEAVVVLGKFFK
                     MEPGKDYKVITFNAETDRVAALINKSIDAALLSFPHAARAARAGMKILVKTGDYIPRL
                     GGTFITHRDFLKEKRDVTKRFIRAMVRANDYVKANKQGTIEVIQKYFEIKEPGLAEGI
                     YNQVANAFGPEIPHDLLLELFQSRTTPELGWPAGKPLPDIEQFVARDLLNEVLKEMGR
                     KVTK"
     gene            1517..2467
                     /locus_tag="VFY96_01770"
     CDS             1517..2467
                     /locus_tag="VFY96_01770"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5605209.1"
                     /translation="MNILITGAGLIGSNAARHAVDNGHKAVLFDLSPNRDYIDKVVGR
                     DKADIVAADMRDLPALLAAMEKFNVNTIVHTAGLIGSRVQENSYTGATNNILGTINIL
                     EAARLRKVRRVVYVSTFGAYDRNKINDNVIRETHPIGGRNLYATTKVCSEHLIHAYEG
                     LYNLDVIIIRPGGVFGRGFYVGGSTVGKVMRDLALNIIKGRPFTIDAKTYGPNEYVYG
                     KDVGMALYLACEAKNPKQRIYNAGTGVVHGPEELAQVVRELLPNAEIKVSGTSGAEKS
                     RSIPMDISVSKAELDYAPKFMLKDALRDYMDELWADGARP"
     gene            complement(2494..3204)
                     /locus_tag="VFY96_01775"
     CDS             complement(2494..3204)
                     /locus_tag="VFY96_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5605210.1"
                     /translation="MKLSVLMPVYNEERTLDEIVRRVCAINIPKELILVDDGSKDRSR
                     EILTRLHEENRRAGDPLNEIKIFFQPQNEGKGSALKTALDHVTGDVVMIQDADLEYDP
                     KDYPSLLQPIQEGVADVVYGTRFAGGGAHRVHFFWHSMGNRMLTLLSNMLTNLNLSDM
                     EVGYKVFRADVLKGINLQSKRFGFEPEVTVKLAKKGCRFYEVPISYYGRTYQEGKKIT
                     WKDGLAALYYMIRFRFGN"
     gene            complement(3201..>3411)
                     /locus_tag="VFY96_01780"
     CDS             complement(3201..>3411)
                     /locus_tag="VFY96_01780"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX5605211.1"
                     /translation="VPHYLSVSPNPRGTLALLLRLSQWLGLRVDTSPLERAAADFQAK
                     INDAVNGDPKLSAFVRELKKREFEQ"
BASE COUNT          755 a          977 c          897 g          782 t
ORIGIN      
        1 ttggagaatt ttttggtgcg agcaagggtc tcggcttcat gatcgccacc gcggccacga
       61 gctacaaagt ggacgtagta ttcgccggcg tggcgatctt tatgggcctc tctctgatcc
      121 tgacgatggc ggtaaagaaa atcgaatcca atctctcgcg ctggcgcccg gagggggcca
      181 agaccttcta gtgggctgtg acagcaattt agcgttcgcc gttcatgctt cgcttcgacg
      241 ttgctcaggg caggctgtct cagcacgaat ggcttgcgcc ttgaccgatc aacctgagct
      301 tgcctaacgg ttcttagaat ttagccgcga atcaatggtt acagttcact aatatcttac
      361 ttggtcacct tcctccccat ctccttgaga acttcattca agagatcgcg tgcgacgaac
      421 tgctcgatat cgggcaaagg cttgcccgcc ggccagccaa gctccggcgt cgtccgggac
      481 tgaaaaagtt ccagcagcag atcgtgagga atttccggac caaaggcgtt cgccacctga
      541 ttgtatattc cctcggccaa cccgggctct ttgatttcaa aatatttctg gatcacctcg
      601 atggtgccct gcttgttggc cttgacgtaa tcgttcgctc taaccatcgc gcggatgaac
      661 ctcttggtca cgtcgcgctt ctctttcaga aaatcccggt gggtaataaa tgtaccaccg
      721 agtctcggaa tgtaatcccc cgttttcacc aggatcttca taccagccct tgccgccctc
      781 gccgcatggg gaaacgagag caaagcagcg tcgatcgatt tgttgatcag cgcggccacc
      841 cgatcggtct cggcgttaaa ggtgatcacc ttgtagtctt tgcccggttc catcttgaag
      901 aactttccga gcacgaccac ggcttccgta taagcagcct gccccggcct ctcaagacct
      961 atggtctttc ctttcaactc ctccactcgc ttgaattcgg gctgggtgac gatagcccat
     1021 tgagaaacca gggactcgcc gacaataaaa gcgagcggcg ctcctttgag catcgcctca
     1081 gcgcctccac cgggaatcgg cacgaactca atttctttag cgatgcctgc cctgactaaa
     1141 acactcgcgg tagctgaaat gaagtctgcg tttagccctt ccgctttcaa cagtcccttg
     1201 tccatggcta tgtaggacgg gatgtggaag ccgctgccgc cgatcgtctt gccgattttg
     1261 atcgattgcg cgcatgcaga atacgctgac aataacagga ttggcgcggt ggaaaaaatt
     1321 gccaccagcg agcagaaaac ccaccgtctt cgcacgagat tgagccgatt catggagtcc
     1381 cccttttcgc gtaagccgac gataggaaac catataatcc tgagtgacca gcacaggcaa
     1441 ccccgcgcca tcctgtttga cagtgctccg acgcctgcct aatgtgggcg tgcaaacgat
     1501 ccgggaggaa aagcccatga acattctcat taccggagcc ggattgatcg gcagcaacgc
     1561 ggcccgccat gccgtcgaca acggccacaa agccgtcctt ttcgatttgt cgccaaaccg
     1621 cgattacatc gacaaggtcg tcggtcgaga taaagctgat atcgtcgccg ccgacatgcg
     1681 cgatttgccc gcgttgctcg cggctatgga aaaattcaac gtcaatacta tcgttcatac
     1741 cgccgggttg atcggctccc gcgtacagga aaattcttac accggcgcga ccaacaatat
     1801 actcggcacg attaacatcc tcgaagctgc gcgtctgcgc aaagtacgcc gtgtggttta
     1861 cgtcagtacc ttcggcgcct atgatcgaaa caagatcaac gacaatgtga tccgtgaaac
     1921 tcatcccatc ggcgggcgca atctctacgc caccaccaag gtctgctccg agcatttgat
     1981 tcatgcttat gagggcttgt acaacctcga tgtgatcatc atccggccgg gcggcgtttt
     2041 cggccgcggt ttttacgtcg gcggctcgac ggtcggcaag gtgatgcgcg atctggcgct
     2101 gaacatcatc aaaggccggc cgtttaccat cgacgctaaa acttacggcc ccaatgaata
     2161 tgtttacggc aaggatgtcg gtatggcgct ctatctcgca tgcgaagcga agaatcccaa
     2221 gcagcgcatt tacaacgccg ggaccggcgt agtccacggt ccggaagagc tcgctcaggt
     2281 cgtcagagaa ctgctgccca acgccgagat caaagtcagc ggcaccagcg gcgccgaaaa
     2341 gagccgcagc attccgatgg acatcagcgt atcgaaggcg gagttggact acgcgccgaa
     2401 gttcatgctg aaagacgcgc tgcgcgatta tatggatgag ctatgggccg atggagcgcg
     2461 tccttgaaac tggaggacgc ttgttggaga cctttaattt ccgaagcgaa accggatcat
     2521 gtagtacagc gcggcgagcc catctttcca agtgatcttt ttaccttctt gataggtgcg
     2581 cccgtagtat gagatcggta cctcgtaaaa gcggcagccc ttcttcgcca acttcaccgt
     2641 gacctccggc tcgaagccga atcgtttcga ctggagattg atgcccttca atacgtcggc
     2701 gcgaaagact ttatagccga cttccatgtc ggagagattc aaattcgtga gcatattgga
     2761 aagcaaggtc agcatccggt tgcccatgga gtgccagaaa aaatgcacgc ggtgcgcgcc
     2821 gccgcccgcg aagcgcgtgc cgtaaacgac gtcggccact ccttcctgga tcggctgcaa
     2881 taagcttggg taatccttcg gatcgtattc cagatcggcg tcttggatca tcaccacgtc
     2941 gccggtcacg tgatccagcg cggttttcaa cgccgagcct ttgccttcgt tctgcggctg
     3001 aaaaaagatc ttgatctcat tgagcgggtc gccggcgcgg cggttttctt cgtgcagtcg
     3061 cgtcaagatc tcccggctcc gatcttttga accatcatcc acgaggataa gctccttcgg
     3121 gatgttaatg gcgcaaacac gccggacaat ctcgtccaac gtccgctctt cgttgtacac
     3181 gggcatcagg acggaaagct tcactgttcg aattcgcgtt ttttgagttc ccggacgaag
     3241 gcggagagtt tgggatcgcc atttaccgcg tcatttattt tggcttgaaa atctgccgcg
     3301 gctcgttcca atgggctcgt atcgaccctt aggccgagcc attgactcaa gcgtaacagc
     3361 agcgccagcg ttcctctggg attaggcgag acggataaat aatgaggcac t
//
LOCUS       DASULZ010000088         2266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_415606, whole genome shotgun sequence.
ACCESSION   DASULZ010000088 DASULZ010000000
VERSION     DASULZ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415606"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..320
                     /locus_tag="VFY96_01785"
     CDS             81..320
                     /locus_tag="VFY96_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605212.1"
                     /translation="MLHEADVDKVAIEAASNSDDIRTTVYLSNVPNRDAGVALAKKVH
                     AEALNRISFWHELGIEDAAPTGHQFTPINSAAGIL"
     gene            477..749
                     /locus_tag="VFY96_01790"
     CDS             477..749
                     /locus_tag="VFY96_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605213.1"
                     /translation="MHLYNILLMFFDDERPRAQERLDTYIREQNSDVPMTPNPRTNRP
                     ETVYTRLRNEFGHHRQGVNLDDTKAQMEKRLGELIGLSNQVIESRR"
     gene            complement(1209..1285)
                     /locus_tag="VFY96_01795"
     tRNA            complement(1209..1285)
                     /locus_tag="VFY96_01795"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1249..1251),aa:Arg,seq:tcg)
     gene            complement(1289..>2266)
                     /locus_tag="VFY96_01800"
     CDS             complement(1289..>2266)
                     /locus_tag="VFY96_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605214.1"
                     /translation="LTDGTVRLARGSYPVAYDSAFNQRMVVDPDSKISASLAAKLSHP
                     IIGAPVLSFGNGETMRDAVGQLLAGLGYQALPADQSIVIQEEGVTFEAKGDWVVLAPE
                     VSNKTQEMYVINLTEKSNQTPGYLKSELAKKGLHLREVALKPESLIDPRSAENNQSLI
                     AGGQTKNWPRDKQELVDALLLLYGVTFGVAEKLPVELGDGVEVDVGVDRFFELGGQRT
                     ALFFNGGSPEVRKLLEEKRGITPIVLDLASMSPREIITRMLNLLGDPTVYREHRFSAG
                     GDALPDRITIRAWGFQPTKKTIFITDRQIPPTFQRFFFEKGLDIVYFRQ"
BASE COUNT          563 a          611 c          579 g          513 t
ORIGIN      
        1 agatcgtgaa caaggaaaaa acttccccga tagcattgac ccccgcgacc gttaaatcac
       61 ggtgattttt tgctaatata atgttgcacg aagcggacgt cgataaagtc gcgatagaag
      121 ctgcaagcaa cagtgacgac atacgcacaa ccgtttacct gtctaacgtg cccaatcgcg
      181 acgctggcgt tgcgctcgcg aaaaaagttc acgcagaggc tctgaaccga atctctttct
      241 ggcacgaact cggcatcgag gacgctgcac ccaccgggca ccagttcacg ccgattaact
      301 cggccgccgg aatcttatag ccattgaagc ggcggacacg atgaactttt acagcgatgc
      361 ttcaaaagtc gttcttggct tggaggccgc cagattgaaa gcggatcttg agcaacgacc
      421 ctggccggag aggattattt cggcctcttg ctgtccacaa gcccgtcgag aagtttatgc
      481 atttgtacaa cattctgctg atgttcttcg atgatgagcg accgagagca caggagaggc
      541 ttgatactta tatccgggaa caaaactcag acgtgccgat gacgcccaat ccgcgcacaa
      601 atcgacccga gacggtttat acccggctca gaaatgaatt tggccaccac cgccaaggcg
      661 tgaaccttga cgacacgaaa gcacaaatgg aaaagcggct cggcgaattg attggattga
      721 gtaaccaagt tatcgagtcg cgtcgctgag cggccacaaa atgaccatca tcaggtgccc
      781 tcgctgcaag agcacgatga tcgcccgctc gcaccgccgg aattttctgg agaggctgct
      841 taagttcgtt tggatcaggc cgtatagctg tacgaaatgt ggacggcggt ttggggattt
      901 aggcggtaaa ctaagattga aatttggacc catccgatga ggctgtacaa acgtttacgg
      961 gtaatcacgt gcccgaattg caagagcgac ttgatcagcc ggtcacgccg ccacgactta
     1021 ctggaaaggt tattacgatt ggtctcgatt aggcagtatc gctgcgggaa ctgcgaacag
     1081 cgattctggg cgcgacgcat acggtaacca tcggcccttg agccgctacc gctgctacca
     1141 ctttggaaaa aaatgtggaa acaatttggg aatgcggctg atttgtggtc gtaacttgct
     1201 ggatttaatg gcgcgcctgg agggattcga acccccaacc ctcagatccg aagtctgatg
     1261 ctctgtccag ttgagctaca ggcgccgttc attgtcgaaa atagacgata tcgagacctt
     1321 tttcaaaaaa gaaacgctga aacgtcggcg gaatctgacg gtctgtgatg aatattgttt
     1381 ttttcgtcgg ctgaaatccc caagcgcgaa tcgttattcg atctggcaac gcatctcccc
     1441 cagcggaaaa gcgatgttcc cggtaaactg tcggatcacc tagtagattc aacatgcgcg
     1501 ttataatctc tcgtggcgac attgatgcca ggtcgagcac aattggcgtg ataccccgct
     1561 tttcctcgag caatttccga acttccgggc tcccgccgtt aaaaaatagc gcggtgcgct
     1621 gcccacccaa ttcaaagaag cgatcgacgc caacgtcgac ttctacgccg tcgccgagct
     1681 ccaccggcaa tttttccgcg acgccgaaag tgacgccgta taacaatagc aaagcatcaa
     1741 cgagctcctg cttatcgcgc ggccagtttt ttgtctgacc gccagcaatt aagctctgat
     1801 tattttcagc actccgcgga tcgatcaggc tttcgggttt aagcgccacc tcacgcaggt
     1861 gcaatccctt ttttgccagc tcggatttaa gatatcccgg ggtttggttg gacttctcgg
     1921 taagattgat aacgtacatt tcctgagtct tgttgcttac ctcaggggca agaacgaccc
     1981 aatcaccttt tgcctcaaag gtaacgccct cctcctgaat gacgatcgac tgatcagccg
     2041 gcaaagcctg gtaacccaat cccgcgagca gctgcccgac cgcatcgcgc atcgtctcgc
     2101 cattgccgaa cgacaagacc ggggcaccta ttattgggtg actcagtttg gcggcgagag
     2161 atgccgagat cttgctgtcg ggatcgacaa ccattctctg attgaacgca gaatcgtagg
     2221 ccaccggata ggaacctctt gcaagacgca cggttccgtc cgtcaa
//
LOCUS       DASULZ010000089         7837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_618741, whole genome shotgun sequence.
ACCESSION   DASULZ010000089 DASULZ010000000
VERSION     DASULZ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7837
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618741"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..1230)
                     /gene="aroG"
                     /locus_tag="VFY96_01805"
     CDS             complement(151..1230)
                     /gene="aroG"
                     /locus_tag="VFY96_01805"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HEX5605215.1"
                     /translation="MQYRTDDLRIKEIKELLPPAKLLGDFPITEKAAQTVYETRQKIH
                     RILHGADDRAVLIAGPCSIHDTKAAQEYATLLKEAEDRFAPDLLILMRVYFEKPRTTV
                     GWKGLINDPNLDGSFQINDGLRLARRLLLELNEAGMPAGCEFLDMITPQYFADLVSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGNVRIAMDAIRAAQAPHHFLSVTKAGHS
                     AIVSTAGNEDCHIILRGGKQPNYDAGNVDAAAKNLAQAGIPARIMIDCSHGNSGKDPA
                     NQVKVGRDVTAQIAAGDERIFGIMIESHLKPGRQDLLPGKPLVYGQSITDACVGWEDT
                     RVLIDALAGAVRQRRLKAAANGGSD"
     gene            complement(1446..2669)
                     /locus_tag="VFY96_01810"
     CDS             complement(1446..2669)
                     /locus_tag="VFY96_01810"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5605216.1"
                     /translation="MRSAVNFTPTVRGLTTLYTSALLAGMWSMMVPAVPVIARAFEVS
                     PGTAAQAITALALGRFAGMPISGAALDRLGTRAALISGPVIACSAATLAGMSPSFGVI
                     LTLLFLIGVGESIWVIAREVAGIDLARQDQRGRVLSGFHGINNLGLALGPLFGGLLTE
                     ALGFRSVFFSYAVCALAAAFLGTSVNGSGVAPISSPPFNWSLAALGQRLRGLKDLFHQ
                     IEPALRATYVVLVFATLTSFMHRVTTQSILPLLATSKLGFSPKEVGLLFTISGFSVFA
                     MILPAGFVIDKVGRKWATVPSTGIPALAFLLIPFAESFFQLAVLISFLGIANGLSLGS
                     LATSTYDVVPAHARGRLQAARRTVAEIGGVAAPLFGGYLADSFNPGVPFLVYAPLLIL
                     SAILLALVGRETLVR"
     gene            2933..5440
                     /locus_tag="VFY96_01815"
     CDS             2933..5440
                     /locus_tag="VFY96_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605217.1"
                     /translation="MLFYLGVFLISFGALALEIVQTRILSVVVWYHLAFFVISLAMFG
                     LTAGAVWVYLKGDRFSDKTLSYDLGYFSGLFAIATAVCLATQMTLAPIVGRLLTTLWV
                     WTELALCLSIPFFFAGVVLSIALTRSSFSVARVYGVDLLGAAAGAVGALFLLNTTDGP
                     SAVLWIGAIGAAASILFMRSGIGGAPAIKSPLAAAISNRPWIFLFIVLLAIVNPFLEH
                     GLQPIAVKGKFEFPGSKALRRWNSFSRVDVSPTFRGFPIMWGPSPKMLEKKWLTNQVD
                     LFIDGDAGSTAYRFDGNFADVEFLRYDVTNLAYFLPHRERGVVIGMGAGRDVLSAALF
                     GVKEITAVELNPILVDLQTSESGLLPFTNIARIPGVRLIADEGRSWLARNRDFFDVVQ
                     MSLVDTWAATGAGAFTLSENGLYTIEAWKIFLNRLTPNGVLTVSRWYEPAFPEETGRL
                     VSLAVATLMKMGISEPRRHLFLAAQKRIATLIVSTAPLAASDLDALEQAAASYQHEVL
                     ITPSREPASTVLARIVGAPNISALVRYTANLPFDLTPPTDGRPFFFNQLPLSRPLEAL
                     AFARSMLAASSEGGGVRRGNLVATVTLLILFSVALLLVVMALIIPLRSALNDLGARPV
                     THGTVYFSLIGVGFMMIEIALLQRMSIFLGHPVYSLSVLLSTLILTTGIGSFLCDKFP
                     LQTRSRLLLWAAATGGYSIALALLMGPVLTALDGAELPSRIIFCIAVIAPLGMLLGFG
                     FPSGVRLISLVDAKPTPWFWGINGAAGVLASIVALVLSLAFGISVTLSVGGLCYFLLI
                     PSALFLTRAMTSARVKKTSPVRISSGRAAKLPARRSR"
     gene            complement(5484..6875)
                     /gene="asnS"
                     /locus_tag="VFY96_01820"
     CDS             complement(5484..6875)
                     /gene="asnS"
                     /locus_tag="VFY96_01820"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017289279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEX5605218.1"
                     /translation="MTAQLSSVKQAFNLPVGSETALRGWVRSRRDSKGVTFVELNDGS
                     RFKSIQLVVEAGAISDETLKQVTTGSSIEAIGRLVESPAKGQAVELKVTAIQVYGSAD
                     PATYPLQKKGHTLEFLREISHLRVRSNTFGAAFRVRNALTHAIHLFFQARDFLYVQTP
                     IITTSDCEGAGQMFNVTTLNMQQPPRTPDGSIDWQQDFFAKPAYLTVSGQLEGEIFAS
                     AFSKVYTFGPTFRAENSNTPRHLAEFWMIEPEMAFYQLDDNMRLAEEFLKHIIRYVLE
                     QCREDLEFFNQFIEKSVLATLEHVAESDFGHMTYSDAVAELKKSGKAWEFPVQWGNDL
                     QTEHERYLSEEVFKKPVIVTDYPREIKAFYMRLNDDDKTVRAMDVLVPRVGEIIGGSQ
                     REERYEILLRRLRETGLDEKPYWWYLDLRRYGTVPHSGFGLGLERMMMYLTGLKNIRD
                     VIPFPRTPGNAEF"
     gene            6999..7394
                     /locus_tag="VFY96_01825"
     CDS             6999..7394
                     /locus_tag="VFY96_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605219.1"
                     /translation="MATSSTLIPEDVAQFIIEKIDSVAQMEALLLLRENPQQQWHVAA
                     VAKRLYIDDEQAGKVLARLREEELLVAEPSQSIEYRYQPGSLVLSQIVDRVAEIYSKH
                     LVPITNLIHSKPKSRVQEFADAFKFRKDE"
     gene            7396..7653
                     /locus_tag="VFY96_01830"
     CDS             7396..7653
                     /locus_tag="VFY96_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HEX5605220.1"
                     /translation="MAPLFYALCTVTAALCAWLLLRGYFRSGYKLLLWGGLCFVGLTV
                     NNALLVADKQLFPEVNLYPWRLVVALLAMLVLLYGIIWDRE"
     gene            7663..>7837
                     /locus_tag="VFY96_01835"
     CDS             7663..>7837
                     /locus_tag="VFY96_01835"
                     /inference="COORDINATES: protein motif:HMM:NF039620.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HEX5605221.1"
                     /translation="MNAALLGAIAMASLVAGLFFLRFWKDTRDRFFLFFSISFFVEGL
                     NRFALAMVSDPNED"
BASE COUNT         1609 a         2128 c         2258 g         1842 t
ORIGIN      
        1 gcgaccatgc gccaaccgta gaacaatccg gtaaactgct taactatctt ggcggcttga
       61 gtgggaacag gccaaatcat ttcagcaagt gctcagcacc cggcacggca tcgagaatga
      121 atcgggaggt ttcagctgcc aaagagcccg tcagtcggag ccgccgtttg ctgctgcttt
      181 caggcgccgt tgacggacgg cgccagccag cgcatcgatc aagacgcgcg tgtcttccca
      241 gcccacacag gcatcggtga tgctctggcc gtagacgagc ggttttccgg gaagcaaatc
      301 ttggcggcct ggcttcagat ggctttcgat cataatgccg aagatgcgtt catcaccggc
      361 ggcgatctgc gctgtcacgt cccttcccac cttcacttgg ttggcaggat ctttgccgct
      421 attgccgtga ctgcagtcga tcatgatgcg cgccggaata ccggcttgcg caagattttt
      481 ggccgcggca tcgacgttgc cggcatcgta atttggctgt ttgccgccgc gcaggatgat
      541 atggcaatcc tcgttaccgg cagtggaaac gatcgctgaa tggccggcct ttgtgaccga
      601 aagaaaatga tgcggcgcct gagcggcgcg aatcgcgtcc atcgcaatgc gcacgttgcc
      661 gtcggtgccg tttttaaaac cgacaggaca cgagagaccg gatgcaagct cgcggtggac
      721 ttggctctcg gtcgtgcgcg cgccaattgc gccccaggaa accaaatcgg cgaagtattg
      781 cggcgtgatc atgtcgagga attcacatcc cgcgggcata cctgcttcgt tcaattccag
      841 caataaacgg cgcgccaagc gcagtccatc attgatttga aaactgccgt cgaggttcgg
      901 atcattgatc aagcccttcc agcccacggt cgtgcgcggt ttctcaaaat aaacccgcat
      961 gaggatcaat aaatccggag cgaaacgatc ttcggcctct ttcagaagcg ttgcgtattc
     1021 ttgggctgct ttggtgtcgt gaatcgaaca gggaccggcg atcagcacgg cgcgatcgtc
     1081 cgcgccgtgg agaatccgat ggattttctg acgcgtctca tacaccgttt gtgcggcttt
     1141 ttccgtgatc ggaaaatcgc ccaacagctt ggctggcggc aacaattcct tgatctcttt
     1201 gatgcggaga tcgtccgtgc ggtactgcat aactttcgga atggcgcaat cccaccatta
     1261 tagacgaaag ttcgcggaaa tctaacgagc ccagcggcgg taccgactca tttctttaga
     1321 tccttcgctt cgttttagga tgacaacggg tgatccgtta ttttgaggga agccgaagaa
     1381 tcccgctgtg gcagattaac tctgtcagcg taaaatgagt caacctcgac acgcaggggt
     1441 gcgcgttacc gtaccagggt ctcgcgtccg accagtgcaa gcaaaatcgc ggataggatt
     1501 aacagtggcg catagaccaa gaaaggcaca ccgggattga aactgtcggc gagatagccg
     1561 ccaaacaagg gcgcagcaac accgccgatc tccgccactg tgcggcgcgc ggcttgcagc
     1621 cggccacgcg cgtgagccgg cacgacgtca taggtcgatg tcgccagcga gccgagggaa
     1681 aggccattgg cgatgccaag aaacgaaatc agcaccgcga gctgaaaaaa actttcggca
     1741 aacggaatca gtaaaaatgc caaagccgga atgcccgtgc tcggaaccgt cgcccatttc
     1801 ctgcccactt tgtcaatgac aaagcccgcg ggcaggatca tcgcgaaaac ggaaaaaccc
     1861 gagatcgtaa acagcaagcc gacttctttg ggcgaaaaac cgagcttcga agtggcgagc
     1921 agcggcaaga tgctttgggt ggtcactcga tgcatgaagc ttgtcagtgt ggcaaacacc
     1981 aaaacgacat aggtggcgcg cagagccggt tcgatctggt gaaacagatc cttcaagccg
     2041 cgcaggcgct ggcccagagc agccaagctc cagttaaagg gcggactcga gatcggtgcc
     2101 actcccgagc cgttcacgga ggtcccgagg aaggccgccg cgagggcaca gaccgcgtag
     2161 ctgaaaaaaa ccgagcggaa gccgagcgct tcggtaagca gtccaccgaa cagtgggcct
     2221 aaagcgagac cgagattgtt aatgccgtga aatccgctca gcacccggcc acgctgatcc
     2281 tggcgcgcaa ggtcgatgcc ggcaacttca cgcgcgatca cccaaatact ttcgccaacg
     2341 ccgatcagaa acagcaatgt caatatcacc ccgaacgatg gactcatgcc ggctagcgtg
     2401 gcggcgctgc acgcgatcac ggggccggag atcaaagcag cgcgcgtacc gagccgatcg
     2461 agcgccgcgc cgctgatcgg cattccagca aaacgcccga gagcgagggc cgtgatcgct
     2521 tgcgccgccg tgcccgggct gacctcgaat gcccgtgcga tcacgggcac cgccggcacc
     2581 atcatcgacc acattccggc aagcagagcg ctggtataaa gcgtagtgag gccgcgcacg
     2641 gtcggggtga aattaactgc ggatcgcatc agcttgcagc aagcagtcgg gttcgcatgg
     2701 tcgtttactt attcgtgacg ctgctggatg gcgccggctg cgatttcaac gtcgtaattt
     2761 tttccggcgc gtgctaatga atgcaaaacg gaaactcaga tgcgcgggaa aacctcattg
     2821 tttttacttg ccatgcatct cggttccgag caaggtgttc gaatgactgg ggttggttgc
     2881 cgctcaatgc acgttgtcta tttcgagtaa tcagaccctc cttacgaagc acatgctttt
     2941 ttatctcggc gtattcttga tcagctttgg cgcgttggca ttggaaattg tgcagacgcg
     3001 gattctgtcg gtggtggtct ggtatcacct ggcgtttttt gtgatcagcc tggcgatgtt
     3061 cggtctcacg gcaggcgcgg tttgggttta tctgaagggc gatcggttca gcgacaagac
     3121 gctgtcttac gacctcggct atttcagcgg gctatttgcg atcgcgacgg cggtttgcct
     3181 ggcgacgcag atgacgcttg cgccaatcgt cggtcgtttg ctcacgaccc tttgggtttg
     3241 gacagagctg gcgctgtgct tatcgattcc ttttttcttt gccggtgtgg tgctgagcat
     3301 cgctctgacc cgcagctctt tttcagtggc gcgagtttac ggcgtcgatc tgttaggcgc
     3361 ggctgctggc gccgtcggtg cgttattcct tttgaatacc accgacggac catccgcagt
     3421 gctttggatt ggggcgatcg gcgcagcagc ttcgatcttg tttatgagat ccggaatcgg
     3481 cggcgccccg gccattaagt cgccgcttgc tgccgcaatt tcaaatcggc catggatttt
     3541 cttgttcatc gtgttgttgg ccatcgtcaa tccttttctc gaacacgggc ttcagccgat
     3601 cgcggtcaaa ggaaagttcg aatttcccgg ctctaaggcg ctgcgtcggt ggaattcgtt
     3661 ttcccgcgtg gatgtgagtc ccacgttcag gggctttccc attatgtggg gtccatcacc
     3721 gaaaatgttg gaaaaaaaat ggctcaccaa tcaggtggat cttttcattg acggtgatgc
     3781 cggcagtacg gcgtatcggt ttgacggcaa ttttgccgat gtcgaattct tgcgctatga
     3841 cgttacgaac ctcgcctatt ttcttccgca tcgcgaacgc ggcgtcgtaa tcggcatggg
     3901 agcggggcgc gatgtactgt cggcggcgct tttcggcgtg aaagaaatta ccgcggtgga
     3961 gctcaatccg atcctcgtcg atttgcaaac cagcgaaagc ggtctgttgc cgttcacaaa
     4021 tattgccagg attcccggcg tccgtctgat cgcggacgaa ggccgcagct ggttggcgcg
     4081 aaaccgggat ttttttgatg tggttcagat gagcctggtg gatacctggg cggccaccgg
     4141 cgcgggagct ttcactttga gcgagaacgg gctttatacc attgaagcgt ggaaaatctt
     4201 tcttaaccgt ttgacgccca atggcgttct caccgtgagt cgctggtacg aaccggcatt
     4261 tccggaggaa accggcagac tggtcagttt ggccgtcgcg acgttgatga agatgggtat
     4321 cagtgagccg cggcggcact tgtttctcgc ggctcagaag cgcatcgcga ctttgattgt
     4381 gtcgaccgcg ccgctggccg catcggatct cgacgccctc gagcaggcgg cagcgtcgta
     4441 tcagcacgaa gttctgatta ccccgagtcg tgagccagcc tcgaccgtgc ttgcgcgcat
     4501 cgtcggagcg ccgaatattt ctgcgcttgt gcgttacaca gcaaacctgc cgttcgattt
     4561 gacgccgccc actgatggcc ggccattctt tttcaatcaa ttgccgctgt cacgtccgtt
     4621 ggaagcgttg gcgtttgccc ggagtatgct tgcggcatcc agcgaaggcg gcggtgtgcg
     4681 ccgtggtaat ctggtcgcca ccgtcacgtt gctgatcttg ttttcagtcg ccttgctgct
     4741 ggtggtcatg gcgctcatca ttccgctgcg ctcggcatta aacgacctcg gcgctcggcc
     4801 ggtgacacac gggacagttt atttttctct gattggggtc ggctttatga tgatcgagat
     4861 tgcgctgttg caacgcatga gcatctttct cggccatccc gtctactcgt tgagcgtgct
     4921 tctgtcgacg ctcattctaa cgaccggtat cggcagtttt ctttgcgata agtttccgtt
     4981 gcaaacccgg tcccgattgc tcctctgggc cgccgcaaca ggaggttact cgattgcttt
     5041 ggcgctgctc atgggcccgg tgttgactgc gcttgacggc gccgagcttc cgagccgaat
     5101 cattttttgt atcgcagtca tcgcgccgct tggaatgttg ctcggctttg gcttcccgtc
     5161 gggggtgcgc ttgatatcat tggtggatgc gaaaccgacg ccatggttct gggggatcaa
     5221 cggcgcagcc ggtgtgcttg catcaatcgt tgcgctggtt ctcagtctgg cgttcggaat
     5281 cagcgtgact ttgagtgtgg gcggattgtg ttacttccta ttgattccat cggcgctgtt
     5341 tttgactcgt gcgatgacga gcgctcgggt aaaaaaaacc agcccggtgc gcatctcgtc
     5401 tggtcgtgca gcaaaattac cggcgcgccg ctcacgttga aatgtaggag ggagaagttt
     5461 cccggttgcc ggggcgatct caactaaaac tcggcgttac ccggcgtgcg cggaaaggga
     5521 atcacgtcgc ggatgttttt gaggccggtg aggtacatca tcattcgttc caatcccagc
     5581 ccgaacccgg agtgaggcac cgtgccgtaa cggcgcagat cgagatacca ccaatagggc
     5641 ttttcgtcca agccggtctc ccgcagcctg cgcaataata tttcatagcg ctcttcacgc
     5701 tgactaccgc cgatgatttc gccgacgcgc ggcacgagca cgtccattgc gcgcaccgtt
     5761 ttgtcatcat cgttgaggcg catgtaaaat gccttgatct ctcggggata atccgtgacg
     5821 atgaccggct ttttgaatac ctcctcggat aagtagcgct cgtgctccgt ctgcaaatcg
     5881 ttgccccatt gcacaggaaa ttcccaggct tttccagatt tctttagctc cgcgacggca
     5941 tcgctatagg tcatatggcc gaaatcagac tcggcgacat gctccagagt agcgagcact
     6001 gatttttcga tgaactggtt gaagaactcc agatcctcgc ggcactgttc gagcacatac
     6061 cgaataatat gcttcagaaa ctcctcggca aggcgcatgt tgtcgtcgag ctggtaaaag
     6121 gccatttcag gctcgatcat ccagaactcg gccaagtgcc gcggcgtgtt gctgttttca
     6181 gcgcgaaatg ttgggccgaa ggtgtaaact ttggaaaatg ctgaagcgaa gatttcaccc
     6241 tcgagctggc cgctcacggt gagataggca ggcttcgcga agaaatcctg ctgccaatcg
     6301 atactaccgt ccggcgtccg cggcggttgc tgcatattca gggtggtcac gttgaacatc
     6361 tggccggcgc cttcgcaatc cgaagtcgtg atgatcggcg tttgcacgta gagaaaatcg
     6421 cgcgcttgga aaaacaagtg aatcgcgtgg gtcagcgcat tgcgcacccg gaaggccgcg
     6481 ccgaaagtat tgctgcgcac gcggagatgc gagatctcgc gaagaaactc cagcgtgtgc
     6541 cctttttttt gcaggggata agtcgccgga tcggctgaac cataaacctg aatcgccgtg
     6601 actttaagct cgaccgcttg ccctttcgcc ggagactcga ccaatcgacc gatcgcctcg
     6661 atgctgctcc cggtggtgac ctgtttcagc gtctcatcgc tgatcgctcc cgcttctact
     6721 acgagctgta tgcttttgaa gcgagaaccg tcgttcaatt cgacgaaagt aacgccttta
     6781 gaatcacggc gcgaccgaac ccacccccgc aaagcggttt cacttccgac gggtaggttg
     6841 aacgcttgct tgactgaaga aagttgtgcg gtcatataaa gtcagtatcg gctaacgact
     6901 cacaatgtca acggacggct ccgctgtgct tgtccattat acatctctgt gcacaactgc
     6961 agggccgatg atgatttccg tttcgagaaa gtgtttccat ggccacgagc tcaacgctta
     7021 tacctgaaga cgtagcgcaa ttcattattg aaaaaatcga ctctgtagca caaatggaag
     7081 cgctgttgct gttgcgcgag aatccgcagc agcagtggca tgtggctgcc gtcgcgaaaa
     7141 ggctctatat cgatgacgag caggctggta aagttttggc gcgtctgcgc gaggaagaac
     7201 tactagttgc cgagccgagc caatcgattg agtatcgata tcagcccggt tcccttgtgc
     7261 tgagccagat agtcgatcgg gtggccgaaa tctattcgaa acatcttgtc ccgatcacga
     7321 atcttatcca ctccaaaccg aagagcagag ttcaagagtt cgccgacgcg ttcaaattca
     7381 gaaaggatga gtaacatggc tccgctgttc tatgcgcttt gcactgtaac ggcagcgctt
     7441 tgtgcctggc tgctgttgcg cggctatttt cgaagtggct acaaattgct gctgtggggc
     7501 gggttgtgct ttgtcgggct gacggtcaac aatgcgcttt tggtcgcgga caagcagttg
     7561 tttcccgagg tgaatcttta tccttggcgt ctggtggtgg cactgctcgc catgctggtg
     7621 ctactttacg gcatcatctg ggacagggaa tgaggtgagg ctatgaatgc tgctttgctc
     7681 ggcgccattg cgatggcttc actggttgca gggctattct ttctgagatt ctggaaagat
     7741 accagagatc ggttctttct cttcttctcg atttcgtttt tcgtcgaagg tcttaaccgt
     7801 tttgcgctgg cgatggtcag cgacccgaat gaagacc
//
LOCUS       DASULZ010000090         3678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_822278, whole genome shotgun sequence.
ACCESSION   DASULZ010000090 DASULZ010000000
VERSION     DASULZ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822278"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3149
                     /locus_tag="VFY96_01840"
     CDS             <1..3149
                     /locus_tag="VFY96_01840"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HEX5605222.1"
                     /translation="RDVVVAEMIGSISETQRMELRALIRLASEIIAYYWPMRTFVHHN
                     PLHGLEELHFEKAVRRGERLLGAQPYLSNETFRDYFQSGRISSDHVDAVLRAYAGNEQ
                     VAIGTQTVSRFDVQRIHLMQGIVAPPAETLAGSVASHSDGALISSLADHLLADSKPGQ
                     SRVTPPEDGTLEKTETVSAWCDRLLGTEIGEAINRELVKWCEAFLDEGHAAWPMPARE
                     KGFYGAWRFLAGQEWSPCGIGNHRQKLARLPANPEASLLESLTAIGINRENWQEYLSL
                     HLAALSGWAGFIKWRADQNDYEWQLAYPIDLAQYLAVRLWYERELVQQACRERLGIDG
                     NVAAISAEIRQRKDLAAIAGSKRAVDRVHITAAWKLVALAQALQIDPSLLVKTSSETL
                     FTIIEWLEHFPESEHGPIWLKAFEAGYREELLSKLELNLSSTEPPTCKDAEEPREPNG
                     DSAGAVRPQAQAVFCIDVRSETFRRHLEAAGDYETYGFAGFFAVFIRHQALGSHHETD
                     QFPVIMKSKNIVREIPRSYHGRHLFRHRHREKLLETGHTLLYDLKENVVTPYVMVESL
                     GWFYILPFLGKTLSPRWYRTALEWVRRKLVPPIATTLTVEKLAREEVVEMIAAEQRAV
                     TRRALQEKFGDRNLNLSLERLEFLRRRALDEFVGEPSPRARATALTPEEESTFIEDLR
                     QHYGINQGAFFARMERITRTGFTLNEQIFTVETVLRVIGLTRNFARLVFFCGHGSSSE
                     NNPFEAALDCGACGGNSGKPNARVLAIMANRPLVREQLAKNGIDIPQDTYFIAGQHNT
                     TTDEVELFDLEDLPPTHRKDLLQLVHDLERAGARTSQERYARFPEGKVLRRASRAKRE
                     TRRRSADWSQVRPEWGLSGNAAFIIGRRALTRGIQLAGRVFLHSYDYREDPDGKLLEV
                     VMSGPQVVGQWINMEHYFSTVDQDVYGSGSKIYHNIVGRFGIMSGPQSDLRTGLAAQT
                     VMNGSRPYHEAMRLLTLIEAPRETIEKIVRNQRHLQELYDNEWVSLIAIEPEERTYYR
                     YVPKIGWTPVETRASALE"
     gene            3174..3512
                     /locus_tag="VFY96_01845"
     CDS             3174..3512
                     /locus_tag="VFY96_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005005276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX5605223.1"
                     /translation="MTDLTLHPMKEIRIIIQGEHLKFATDLLDDKATGYTIIHNISGK
                     GHHGFHTAHPMFNEMDSIIMLMTVVTADQVEPILAGLKPLFARYTGVMFVSDVAVSRL
                     EYFSPASVKT"
BASE COUNT          837 a         1064 c         1025 g          752 t
ORIGIN      
        1 ggcgagatgt cgtcgtggca gaaatgatcg gctcgattag tgaaacccag cggatggagc
       61 tcagagctct gatccgtttg gcgagtgaaa tcatcgccta ttattggccg atgcgcacgt
      121 tcgtccatca taacccgctg cacggtctgg aagagctcca cttcgaaaaa gcggtgcgca
      181 gaggcgaacg cctgctcggc gcacagccct atttatccaa tgagacgttt cgcgactatt
      241 ttcagagcgg caggatttcg agcgatcacg tcgatgccgt cttgagagca tatgccggca
      301 acgaacaggt cgccatcgga actcagacag tcagccgttt cgatgtgcaa cggattcatc
      361 tgatgcaggg aatcgtcgcg ccgcccgcag aaacgctcgc cgggtctgtc gcgtcacatt
      421 cagatggcgc actcatttcg agccttgcgg atcatctgct cgcggactcc aagccaggac
      481 aatctcgcgt taccccgcca gaagatggaa ctctcgaaaa aacagagacg gtctccgcat
      541 ggtgcgatcg tctgttggga acggagattg gtgaggcgat caaccgcgag ctggtcaaat
      601 ggtgcgaggc ttttttggac gaaggccatg ccgcatggcc gatgccggcc cgggaaaagg
      661 gcttttacgg ggcatggaga tttctcgccg gccaggaatg gtcaccttgc ggtatcggca
      721 atcatcgtca gaaactcgcc cgcttgccgg caaatccaga agcttctctc ctcgagagtc
      781 tcactgccat cggtatcaat cgggaaaatt ggcaggaata tctttcgctt catctcgccg
      841 cgctttcggg ttgggccggc tttatcaagt ggcgcgccga tcaaaatgac tacgagtggc
      901 agctcgccta cccgatcgac ctggcgcaat atctcgcggt gcgcttgtgg tacgagcgtg
      961 aacttgtaca gcaagcctgc cgcgaacggc tcggcatcga tggcaatgtg gccgccattt
     1021 cggccgagat ccgacagcga aaagaccttg ccgcaatcgc cgggagcaaa cgcgccgtcg
     1081 atcgcgttca cataaccgcc gcttggaagc tggttgcctt ggcgcaagcg cttcagatcg
     1141 atccgtcact gctagtgaaa acttccagcg aaacattatt cacgatcatt gagtggctcg
     1201 aacactttcc cgaatcggag cacggcccga tctggctcaa agcgttcgag gccggctatc
     1261 gcgaagagct tttgtccaaa ctagaactta atctctcgtc gacagaaccg cccacgtgca
     1321 aagacgcgga agaaccgcgg gaaccgaacg gcgattccgc cggtgcagtg cgccctcaag
     1381 ctcaagccgt tttttgcata gacgtgcgct cggagacctt tcgccggcat ctggaagccg
     1441 caggcgacta cgagacctac ggcttcgcgg gtttttttgc cgtgttcatc cgacaccagg
     1501 cgctcggcag ccatcatgaa accgatcagt tcccggtgat catgaagagc aaaaatatcg
     1561 tgcgcgagat tccgagaagc tatcacggtc gacatttgtt ccggcaccgc catcgcgaga
     1621 aactcctcga aacgggccat accctgctct acgacctgaa agagaacgtc gtgactccat
     1681 acgtcatggt tgaatcgctg ggctggttct acattttgcc atttctcggc aaaacccttt
     1741 ctccgcgatg gtaccgaacc gcgctggagt gggtgcgccg gaagctcgtt ccgcccatcg
     1801 ccaccacatt gacggtagaa aaattggcgc gggaggaagt cgtcgagatg atcgcggcgg
     1861 aacagcgcgc cgtgacgcgc cgtgcgctcc aggaaaagtt tggcgaccga aatctcaacc
     1921 tgtcgctgga gcggctggag ttcttacgtc ggcgcgctct cgacgaattt gtgggcgagc
     1981 cttcgcctcg agcacgcgcc actgccctca cgccggagga ggaatcaacc ttcatcgaag
     2041 acctgcgaca gcattacggc atcaatcagg gtgcgttttt tgcgcgcatg gagcggatta
     2101 cccgcaccgg cttcaccttg aacgaacaga tctttaccgt cgaaaccgtc ctgcgcgtga
     2161 tagggctgac ccgaaatttt gcccggcttg tgtttttttg cggccatggc agcagctctg
     2221 aaaacaaccc cttcgaagca gcgcttgatt gcggcgcctg cggcggcaac tcgggaaaac
     2281 ccaacgcgcg cgtgcttgcg ataatggcca atcgaccgtt ggtccgcgaa caactcgcga
     2341 aaaacggcat agacattcca caagacactt atttcatcgc cggccaacac aatacgacca
     2401 ccgacgaagt ggaacttttt gatctcgaag atctgccgcc gacccatcgg aaggatctgc
     2461 tgcaactcgt tcatgacctg gagagagcgg gagctcgcac cagccaggag cgttacgcgc
     2521 gctttccgga aggtaaagtg ttacgccgcg catcgagagc caaacgtgaa actcgccggc
     2581 gcagtgccga ctggagccag gtgcgacccg agtgggggct ttcgggcaac gccgctttca
     2641 ttatcggccg gcgtgcgctg actcgcggga ttcaactagc ggggcgagta tttcttcact
     2701 cttacgacta tcgcgaagat ccggacggca agctgctcga ggtcgttatg tccggtccgc
     2761 aagtagtcgg ccaatggatc aatatggagc attatttctc caccgtcgat caggacgtct
     2821 atgggagcgg cagcaaaatc taccacaata ttgtcggccg gttcgggatc atgtccggac
     2881 cgcagagcga tttacgcacg ggtctcgccg cgcagacggt catgaacggc tcgcgcccct
     2941 accatgaggc gatgcggttg ctcaccctga tcgaggctcc ccgagagacc atcgaaaaaa
     3001 tcgttcgcaa tcagcgccac ttgcaggagc tttacgacaa cgaatgggtt tcattgatcg
     3061 ccatcgaacc cgaagagcgg acgtactacc gctacgttcc caaaatcggc tggacgcccg
     3121 tcgaaacgcg agcttccgcg ctggaataaa gcgttgctaa aggagggata actatgacgg
     3181 acctgacgct gcatccgatg aaagagatca gaatcatcat tcaaggcgaa catctgaaat
     3241 tcgccacaga cctgctcgac gacaaggcga ccggctacac gatcatccac aacatctccg
     3301 gcaagggaca tcatggcttt cacaccgctc atccaatgtt caacgagatg gatagcatca
     3361 tcatgctcat gacggttgtc actgcggatc aagtggagcc gattctcgca ggattgaaac
     3421 ctctgtttgc ccggtatacc ggcgtcatgt tcgtctcgga cgtggcggtg agccgcctgg
     3481 aatatttttc tccggcttcc gtcaaaacat aactccaccc gctagcggat ttcacatacg
     3541 ccgagcttaa ccgtggcgct cggtatatcc tgctcgaaga ctggtgacgg gcggctaaat
     3601 ggaactgcgc agattctgat gagcacgtac gagaggcaaa cggcgaaaac actccacgac
     3661 atgatcattg atcattcc
//
LOCUS       DASULZ010000091         2025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_822416, whole genome shotgun sequence.
ACCESSION   DASULZ010000091 DASULZ010000000
VERSION     DASULZ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822416"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1480)
                     /locus_tag="VFY96_01850"
     CDS             complement(<1..1480)
                     /locus_tag="VFY96_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX5605224.1"
                     /translation="MAVAELDKLRNVGILGQGGSGKTSLGEAMLFAGGATQRLGKVQD
                     GTAVLDHEPEEIKHKVSISTAFHSLSWKKHPITLIDMPGYAAFFADAINCMRAASGAV
                     FVVNPLVGLRVESERLWSRANEERISRLIFVTKMDHEPANVEERLVPIFEGLEAKGVY
                     LQMPIGAEASFKGVVDLVSMKAYLHEADSGKFSEAEIPADLKSAAESWRQKMVEDVCE
                     TDDALLEKFLDGTEPSSEELKKAIREATRERKIFPVLFGSPLHQIGVAELLDAIIDYL
                     PSPMELGEVEGKNPINGESLKRRADPNAPFSAYVFKTVIDPFAGKLSIMRVISGKIAP
                     DMTCYVSNRQLREKTGHLFRLEGKKQEPVKDAVAGEIVAAAKFKDIATGDTLCDEKAP
                     IVYDGAVRFAPNISFALEPKSKADEDKLPQGLHRMMEEDQTIEMHRDEETRDFILSGM
                     GQQHIEIIVEKLKRKYGAEVVLKAPKVPYKETIRASASAQGRL"
     gene            complement(1506..>2025)
                     /locus_tag="VFY96_01855"
     CDS             complement(1506..>2025)
                     /locus_tag="VFY96_01855"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX5605225.1"
                     /translation="KTGMPILYESPKDVGADRIVNGIAAYDKYRDACIIVDFGTATTI
                     DLISKKGEYIGGAIAPGLSISLEALIQRASKLPRIEIVKPKDVVGRNTINSIQAGIFY
                     GYVGLVDGIVQRIQKQHGIRGKVVATGGLAPLVASECSSIDNVDEFLTLEGLRIIHER
                     NALQEIRARAQT"
BASE COUNT          410 a          622 c          494 g          499 t
ORIGIN      
        1 tcaatcttcc ctgcgcgctg gcgctcgcgc gaatggtttc cttgtacggc acctttggcg
       61 ccttgagcac aacctccgcg ccatatttgc gtttgagttt ttcgacgatg atctcgatat
      121 gctgctgccc cataccggaa agaatgaaat cgcgggtttc ttcatcccga tgcatttcga
      181 tggtctgatc ttcttccatc atccggtgca gaccctgcgg cagcttgtct tcatcggctt
      241 tgcttttggg ctccagcgcg aacgagatat tcggcgcaaa gcgcacggcg ccgtcgtaaa
      301 cgatcggcgc tttctcgtcg catagcgtgt ctcccgtcgc gatgtctttg aacttcgcag
      361 ccgcgacaat ttcaccggcc accgcgtcct tgaccggttc ctgtttcttt ccttccaacc
      421 ggaacaaatg gccggttttc tcccgcagct gccgattgct cacgtagcaa gtcatgtcgg
      481 gagcaatctt tcctgagatc acccgcataa tcgagagctt ccctgcaaag ggatcgatca
      541 cggttttaaa cacgtatgca gagaacggcg cgtttggatc agcacggcgt ttgagagatt
      601 caccattgat cgggttcttc ccttccactt cgcccaactc catgggcgac ggcagataat
      661 cgatgatcgc atcgagaagc tcggccacgc cgatctgatg cagcggcgac ccgaaaagaa
      721 ccggaaaaat ttttcgttcg cgggtcgctt cccgaatcgc cttctttaat tcctctgaac
      781 tcggttccgt gccgtcgaga aatttttcca gcagcgcgtc atcggtctcg caaacgtctt
      841 cgaccatttt ttggcgccac gattccgcgg cgctctttaa atccgccgga atctctgcct
      901 cggaaaattt tccgctgtcc gcttcatgga ggtatgcttt catcgacacg agatcgacga
      961 cgcctttgaa actggcctcg gcgccgatcg gcatttgcag ataaacgccc ttcgcttcca
     1021 atccctcaaa aatcggcacc agacgttcct cgacattggc cggctcgtga tccatcttgg
     1081 tgacaaaaat cagccggctg atgcgctcct cgttcgcgcg cgaccacaga cgctcggatt
     1141 cgacccgcag gcccaccagc ggattgacaa cgaacaccgc gccgctagcg gcgcgcatgc
     1201 agttgatcgc atcggcgaag aaggccgcat agcccggcat atcgatcaaa gttataggat
     1261 gctttttcca gctcaacgaa tgaaatgccg tcgagatcga gactttgtgc ttgatctctt
     1321 cgggctcgtg atcgagcacc gcggtcccgt cttgaacctt tcccagtcgt tgcgtcgcgc
     1381 cgcccgcgaa caacatggct tcgcccaacg atgttttacc tgaaccgcct tgccccagaa
     1441 tcccgacgtt acgaagttta tcgagctccg ctacagccat gataatagtt cccttcctcg
     1501 aatctctaag tctgcgctcg cgctctaatc tcctggagcg catttcgttc gtgaatgatc
     1561 cgcaagccct cgagggttaa gaattcgtcc acgttgtcga tactggaaca ttccgaggcc
     1621 accagcggcg ccaatcctcc ggtcgcgacg accttgcctc gaataccgtg ctgtttttga
     1681 atgcgctgca caatcccatc cacgagcccc acatagccgt aaaaaatacc ggcttgaatc
     1741 gagttgatcg tatttcgtcc gaccacgtct ttaggcttga cgatctcgat gcgcggcagt
     1801 ttcgacgctc gctgaatcaa tgcctccagc gatatcgata ggccgggcgc aatggcgcca
     1861 ccgatgtatt cgcctttttt tgaaatcaga tcgatggtcg ttgccgtgcc gaaatcaacg
     1921 atgatgcagg cgtcgcgata tttatcgtat gccgcaatac cgttcacgat ccggtcagca
     1981 ccgacatcct tcggactctc gtaaaggatc ggcatgcccg tcttc
//
LOCUS       DASULZ010000092         4407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_314832, whole genome shotgun sequence.
ACCESSION   DASULZ010000092 DASULZ010000000
VERSION     DASULZ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314832"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(338..1468)
                     /locus_tag="VFY96_01860"
     CDS             complement(338..1468)
                     /locus_tag="VFY96_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605226.1"
                     /translation="MRREREKRRQRQWIANAVASWILLYVAFASAADQANWKQEWARV
                     IEAAKQEGQLSLYGGQEITHPDIIAGFNKDFPFVKVNSASGRAADMMTRIVAERRADR
                     YLADLTASGPNGPRMLYLNKILDPIAPTFILPEVSDPSRWYGGRHWYADPENKYIFMF
                     EGTIVSTGLSFNTKLMTADEITSYWDLLQPKWKGKLLGQDPRGAALSTPVLILYHRAG
                     LGTEFLRRLYTETDITLFRDRRQGTNWLATGKFPVCHLCRDIDKAAKQGLPVEDISPD
                     KLKEGGTIGGGGSSVLALINRAPHPNAAKLFINWYLARQGQALWQHVMNVKEVEASDS
                     MRIDIAKEDVLLDGKRAPGREYQVIGFLDPEPVQKLLQEILK"
     gene            complement(1487..2623)
                     /locus_tag="VFY96_01865"
     CDS             complement(1487..2623)
                     /locus_tag="VFY96_01865"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5605227.1"
                     /translation="MKRVWMLIGMALLLVTFLKSSGLAAQAEAWKGDWEKTIQAAKKE
                     GQLTLYGSPDFEGLFGEFHKKYAEIKITGVFNRGADVAKRLMAERRGDKYLADLYVNG
                     MTTGYNVFYKAKALDPIPPQLILPEVTDTSKWWRGKLHYVDPENQYLLNINGENRMVV
                     AYNTKLVNPAEIKSYWDLLNPKWKGKIVAYDPTLGGSGDAMRFFYHSKSLGPEFIRRI
                     LLETDIVISTDTRQMGDWLAGGKFALSIFGPVSRMDLDLMQMQGLPVDWFKPDQLKEG
                     TYITGGSGGVALINRAPHPNAAKLGLNWLLSREGQIAYQRLFTQGNDGPDSLRIDIPK
                     DKVPRGNRRPEGDENRYPFVDRAEWMDQAPISKFVKEVLEQRKK"
     gene            complement(2632..3726)
                     /locus_tag="VFY96_01870"
     CDS             complement(2632..3726)
                     /locus_tag="VFY96_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605228.1"
                     /translation="MAVFVTATAVYAGEAPNWQTEWEQTLAAAKKEGEITFYGSQGYK
                     KVFEVFQKKYPDIKVNAVTNMRGSEHGARVMTERRAGKYLVDLFINGVVTPIQVYLKA
                     NILEPIRPALILPEVTDESKWWEAKHHYADPDGKYIFVFQGNVHGGENAYNTNSVHPK
                     EIKSYWNFVEPKWKGKIVAYDVSRVSTIAHSLRFLYNHPDLGPEFIRRFFGEMEITYS
                     RDERQMIDWLGAGKFQIAFFVTDIEDAAKQGLPVKFFEPGQFKEGAFVGPSQGGVNLF
                     KNAPHPNAAKVAINWLLSREGQDAYQRMFALQHDVRQSMREDISTDVIPPTYRRIKGV
                     KYIYSGRPEWLDMGPVTKIIKEAQQKSASK"
     gene            complement(3889..>4407)
                     /locus_tag="VFY96_01875"
     CDS             complement(3889..>4407)
                     /locus_tag="VFY96_01875"
                     /inference="COORDINATES: protein motif:HMM:NF044283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605229.1"
                     /translation="LGMPPGISGKSIALSAANVWLALEKAGVTDVKGVWEYQTRYIAV
                     ISIEQKYGGHAKRAAMAMLGSTYGYHRRFVVIVDEDVDPSNIDDVLWAVATRCDPASA
                     INIINEAWSTPLDPRIAPDARAARNFTNSVAIVNACRPYHWKDQYAKTCYFPDETRRR
                     TYEKWKDVLKLD"
BASE COUNT          945 a         1271 c         1091 g         1100 t
ORIGIN      
        1 aatccacatc gccgccgaag agcgactttt ctgccgcttc cgagctattg caaaactcga
       61 aggtgacttt gagtccgttt tgctgccaga ttcccgcctt ctccatcacc tcccagatcg
      121 gcagatgcgc atgagagcgg aacaatccgc gaatctccat cgtgttatgt tccgccatag
      181 ttttcctcct tgtgttgtac cctcgcccgt tcgcggacct catcctaacc ttctcctaaa
      241 ggcagaagga atcctaagat tccgccgtgg aaaatcaacg gctcgtgtaa aatcaaaaat
      301 cccctcggca ttcggatcgg agagagtcag gtgagggcta cttcaaaatc tcctgcaaga
      361 gtttctgcac cggctcagga tcgagaaaac ctatcacctg atattcacgt ccgggagccc
      421 gcttgccatc aagcaacacg tcctccttcg ctatgtcgat gcgcatggaa tccgatgctt
      481 ccacttcctt gacgttcatc acgtgctgcc acagcgcttg tccttgccgc gcaaggtacc
      541 aattgataaa cagcttggcc gcattcggat gcggcgcgcg attgattagc gccaaaacgc
      601 tgctgccacc gccgccaatg gttccgccct cctttaattt gtccggcgaa atatcttcta
      661 ctggcaatcc ctgtttcgct gccttgtcaa tgtcacggca taaatgacaa acgggaaatt
      721 ttccggtcgc aagccaattg gttccctggc ggcggtcgcg gaacagggta atatcggttt
      781 cggtataaag ccggcgcaga aactccgttc ccaaaccggc gcgatgataa agaatcaaca
      841 ccggtgtcga caaagccgcg ccgcgcgggt cctgaccgag aagctttcct ttccatttcg
      901 gctggagcaa atcccaatac gatgtaatct cgtccgcggt catgagcttg gtattaaacg
      961 aaagtcccgt gctcacgatg gtgccttcga acatgaaaat atatttgttc tccggatcgg
     1021 cataccaatg tctcccgcca taccacctgc tcggatcgct cacctcgggt agaatgaatg
     1081 tcggcgcgat cggatcgaga atcttgttga gatagagcat gcgcggaccg ttgggtccac
     1141 tggcggtaag atcggcgaga tatcgatcgg cgcgccgctc cgcgacaatg cgcgtcatca
     1201 tatcggccgc tcgtccgctg gcggaattca ccttcacaaa aggaaaatct ttgttgaatc
     1261 cggcgataat atccgggtgc gtaatttctt gaccgccgta aagcgaaagc tgtccttcct
     1321 gttttgccgc ctcgattacc cgcgcccact cctgtttcca gttcgcctga tccgcagcgc
     1381 tcgcaaacgc gacgtagagc aaaatccaag acgctaccgc gttggcaatc cattgccgct
     1441 gccgtcgttt ttcccgctca cgcctcacgc ctgacgcctc acgtcttcac ttttttcttt
     1501 gttccaagac ttccttcacg aacttactga tcggcgcctg atccatccat tctgcccgat
     1561 cgacaaacgg atagcggttc tcgtctccct cgggacggcg gttgccgcgt ggtactttgt
     1621 ctttgggaat gtcgatgcgc agcgaatccg gcccgtcatt tccctgcgta aacaaccgtt
     1681 ggtaagcgat ttgcccttca cgtgaaagaa gccagttaag ccccagcttc gcggcgttgg
     1741 gatggggtgc tctgttgatt agcgcaacgc ctccggagcc gccggtgatg tatgtgcctt
     1801 cctttagttg gtcgggcttg aaccaatcca ccggcagtcc ctgcatctgc atcagatcca
     1861 aatccatccg tgaaacgggg ccgaatatcg aaagcgcaaa ctttcctccc gccagccagt
     1921 ctcccatctg gcgagtatcc gtactaatga cgatatcggt ttcgagaaga atacggcgga
     1981 tgaactccgg gcccagactc ttgctatgat aaaaaaaccg catcgcgtcg cccgatccgc
     2041 cgagcgtcgg atcgtaagcg acaatcttcc ccttccactt cgggttgagc agatcccaat
     2101 aagatttgat ctcagcggga ttgacgagct tcgtattgta tgcaaccacc atgcgattct
     2161 cgccattgat gttcagcaag tattggtttt ccggatcgac gtaatggagc ttaccgcgcc
     2221 accatttgga ggtgtcggtt acctcgggca gaatcaattg aggcgggatc ggatcgagcg
     2281 ctttggcctt gtagaatacg ttatagcctg tggtcatgcc gttgacataa aggtccgcga
     2341 ggtatttgtc tccgcgtcgc tccgccatca gccgcttcgc cacgtcggcg ccgcggttga
     2401 acacgccggt tattttgatc tctgcatatt tcttgtgaaa ctcgccgaac aatccctcga
     2461 agtcaggact gccataaagc gtgagttgac cttccttttt cgcggcttga atggttttct
     2521 cccagtctcc tttccaggcc tccgcctgtg ccgcgagccc gctacttttg agaaaagtga
     2581 caagtaataa ggccataccg atcagcatcc acacgcgctt catttgttct cctacttgct
     2641 ggcgcttttt tgctgcgctt ctttgatgat cttggtcacc ggacccatat cgagccactc
     2701 cggtcgacct gagtaaatgt atttgacgcc cttaatccgt cgataggtcg gcggaatcac
     2761 gtctgtggaa atgtcttcgc gcatcgattg cctgacatcg tgctgtagcg caaacatcct
     2821 ctggtaggca tcttgtcctt cccgtgacag aagccagttg atggcgactt tggccgcgtt
     2881 gggatgcggt gcgttcttga agaggttgac gccgccctgg ctcgggccga caaacgcccc
     2941 ttctttgaac tgacccggct cgaaaaactt gaccggcaat ccctgcttgg ctgcgtcttc
     3001 gatatccgtc acgaaaaacg cgatttggaa cttgccggca cccagccagt cgatcatctg
     3061 ccgctcatcg cgtgaataag tgatttccat ttcgccgaaa aatcgtctga tgaattccgg
     3121 cccgagatcc ggatgattgt agagaaatcg cagcgaatgg gcgatggtgc tgacccggct
     3181 cacgtcgtac gcgacaattt tacccttcca tttcggctcg acaaaattcc aataagattt
     3241 gatctccttg ggatggaccg aattcgtatt gtacgcgttc tcgccgccgt gaacgttccc
     3301 ctgaaacacg aagatatatt tcccgtcggg gtcggcgtaa tgatgcttgg cttcccacca
     3361 tttcgactcg tcagtgactt cgggaagaat cagcgccgga cgaatcggtt cgagaatatt
     3421 tgccttaaga taaacctgaa taggcgtgac gacgccgttg ataaagagat cgacaagata
     3481 ctttccagcg cggcgctcgg tcatcactcg cgcgccatgc tcgctcccgc gcatgttcgt
     3541 tacggcattg accttgatgt cggggtactt cttttgaaag acttcgaaaa cttttttgta
     3601 tccttggctg ccgtagaatg tgatttcgcc ttctttcttc gccgcagcga gcgtctgctc
     3661 ccattcggtc tgccagttcg gcgcctcccc ggcgtatacc gccgtcgccg taacgaatac
     3721 tgccaccgca acaagccatt ttagattttc gatccttcga ctttgcttag gacaagtttt
     3781 agattttaga ttgtcggaaa aaaacgtaac acgaggaatt aaacgcttgg agctatccac
     3841 gcctacccaa tcgaaaatcc aaaatcgaaa atccaaaatc attttgcctc agtccaattt
     3901 caacacgtct ttccacttct cataggttcg cctgcgggtt tcgtcgggga aatagcaagt
     3961 ttttgcgtac tggtctttcc agtgatacgg ccggcaggca ttgacgatcg cgacggaatt
     4021 ggtgaaattt cgcgcggctc gcgcgtcggg cgcgatgcgc ggatcgagcg gcgtgctcca
     4081 tgcttcgttg ataatgttga tggcactcgc cggatcgcag cgggtggcca cggcccataa
     4141 aacatcatcg atgttggagg ggtcgacgtc ttcatcgacg atgacgacaa aacggcgatg
     4201 ataaccatag gtcgaaccga gcatcgccat ggcagcccgc ttggcgtgac cgccatactt
     4261 ctgttcaatg gaaatgaccg caatatagcg tgtctgatat tcccacacgc ctttcacgtc
     4321 tgtgacgcca gccttttcca acgcaagcca aacattcgcg gcgctgagcg cgatactttt
     4381 tcccgagatg cccggcggca tcccaag
//
LOCUS       DASULZ010000093         2077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_213196, whole genome shotgun sequence.
ACCESSION   DASULZ010000093 DASULZ010000000
VERSION     DASULZ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213196"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VFY96_01880"
     CDS             <1..579
                     /locus_tag="VFY96_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEX5605230.1"
                     /translation="IIRVTKGRTPVRDEELEKTGTVLPTTIPDIEYGRGEIEKQITER
                     TRQKIADFGIELLDERFKRSKYNPAVAEKIIERMSSERHQIAARFRSEGRGEAANISG
                     QKESDVASISSTATKNALEIEGKADAEAAAIYAAAFGAPEAQELYTFLRSLDVMRAAF
                     EKDTTAVLSTNSDLGRMLKSMAEAVAPANPPR"
     gene            676..2049
                     /locus_tag="VFY96_01885"
     CDS             676..2049
                     /locus_tag="VFY96_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010046327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX5605231.1"
                     /translation="MKTTLAHQLADYTCAVRFEDLSKEVVHEVKRRVIDSLGCALGAW
                     KEEPFVIARKVAADFSAKQGSTIFGTDHKSPPDWAAFANGCGIRYFDFNDTYLSKEPA
                     HPSDNISAALAVAESVGAGGRELITATAIAYEVQCRLCDAASIRARGWDHVTYGTFST
                     ALASAKLMKLNSANTRHAVNIAGVAGAAMRQARAGELSHWKGVAFATAARHGVFSAVL
                     ARSGMTGPAPIFEGEMGFEKQLGVSLGNVGEKFHGSPTMILKTSIKYWPAEYHSQSAI
                     ESALFLRDQIGDLSKIKGVVIESHDASVDIIGSEPEKWKPKTRETADHSLPYITAIAL
                     IDGEISDRQFEPERFKDPKVWKFLENVKVERNAELSAMYPDAVGNIVHVDLADGRRLT
                     QRVDYPLGNAKNRLKDSAVEGKFLALVERIIGESRAKKIIELVWKLDEAKNMDELMQA
                     MRMTNDQ"
BASE COUNT          527 a          525 c          611 g          414 t
ORIGIN      
        1 atcattcgtg taaccaaagg gcgaacaccg gtgcgcgacg aagagttgga aaagaccggg
       61 acggttttgc caaccactat tcccgatatc gaatacgggc gcggcgaaat tgagaaacag
      121 atcaccgagc gcacgcgtca aaaaatcgcc gacttcggga tcgaactact cgatgaacgt
      181 tttaagcgca gcaaatacaa tccggctgta gccgagaaga ttatcgagcg aatgtccagc
      241 gagagacacc aaatcgcggc gcgttttcgt tccgaaggcc gcggcgaagc ggcgaatatt
      301 agcggccaaa aagaaagcga cgtcgcctcg atcagttcca ccgccacaaa gaatgcgctt
      361 gaaattgaag gcaaagccga cgccgaagcc gctgcgattt acgctgcagc ttttggcgcg
      421 cctgaagcgc aggaacttta tacgtttcta cgcagcctcg atgtcatgcg cgccgcattc
      481 gaaaaggaca caacggcggt cctctccacc aacagcgatc tcggccgaat gctgaaatca
      541 atggccgaag ctgtggctcc ggcgaatcca ccgcgttgat cggcgtcaag gaggtcgtct
      601 ggatcttgag caggcgcaat gggcgctcct cttgctccag cgtaaatcgt cggcagattg
      661 ggattccaat ttttaatgaa gacaacgctt gctcatcaac ttgctgatta cacgtgtgcg
      721 gtgcggttcg aggatctttc gaaggaagtc gttcacgaag tgaaacggcg tgtgatcgat
      781 tccctgggct gcgcgctagg cgcatggaag gaagaaccgt ttgtgatcgc gcgaaaagtg
      841 gcagccgatt tttcggctaa acaaggatcg acgatctttg gtacggatca caaatcgccg
      901 ccggactggg ccgcattcgc caacggttgc ggcattcgct atttcgattt caacgacaca
      961 tatctgtcga aagagccggc gcatcccagc gacaacattt cggccgcgct cgccgtcgcg
     1021 gaaagtgtgg gcgctggtgg acgcgaattg attacggcca ccgccattgc atacgaagtg
     1081 caatgccggt tatgcgacgc cgcgagcatt cgtgcgcgcg gctgggatca cgtgacttac
     1141 gggacgttct ccactgcgtt ggcgtccgcg aagttgatga aacttaattc ggcaaacacg
     1201 cggcacgcag tgaacattgc cggtgtagcc ggagcggcca tgcggcaggc ccgcgcaggc
     1261 gagctctcgc attggaaagg cgtagcgttt gcgaccgcag cgcgacacgg cgttttctcc
     1321 gcggtgcttg cgcgctcagg tatgaccggg cccgcgccaa tcttcgaagg cgaaatggga
     1381 ttcgaaaagc aactcggcgt gtcgcttgga aatgtgggcg agaaatttca cggatcgccc
     1441 acaatgattt tgaagacgag catcaaatat tggcccgcgg aatatcatag tcagagcgcg
     1501 atcgaatcgg cgcttttttt acgagaccag attggcgatc tttcgaaaat caaaggcgta
     1561 gtgatcgaaa gccatgacgc ttcagtggac atcatcggga gcgaaccgga aaaatggaaa
     1621 ccgaagacgc gcgaaacggc agaccacagt ttgccttaca tcacggccat tgcgctgatc
     1681 gacggcgaaa tctccgatcg ccagttcgag cctgagcggt tcaaagatcc caaggtttgg
     1741 aagtttttgg agaatgtgaa agtggagcgg aacgcggaat taagtgcgat gtatccagat
     1801 gcggtcggca acattgtgca cgttgatctt gccgatggcc ggcgattaac ccagcgcgtc
     1861 gattatccgc tcggcaacgc aaagaatcgg ctcaaagatt cagcggtcga aggaaaattc
     1921 ctcgcgcttg tcgagcgtat tattggggag agtcgggcga aaaagattat cgagctggtc
     1981 tggaaactgg atgaagccaa aaacatggat gagctgatgc aagcaatgag aatgacgaat
     2041 gaccaatgac gaattacgaa ggaatgatcg aatggcg
//
LOCUS       DASULZ010000094         3799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_112168, whole genome shotgun sequence.
ACCESSION   DASULZ010000094 DASULZ010000000
VERSION     DASULZ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112168"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VFY96_01890"
     CDS             <1..726
                     /locus_tag="VFY96_01890"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5605232.1"
                     /translation="EWALTTTYNRWMAEKCASTNGRLRWAAVLPLLQPDKAVEELRWA
                     KEHGACGIYKRGFDLDRNITDRHFFPVYEEASKLDIPLCIHTGHPLPFHEWDRGFPIM
                     YSFSALVSSGVAKKFPRLRFGLIESGASWIPYTISQLGAVKRQSLRGKGTRLPRLYEL
                     EPEIFRQNRIYITIDPIDDIESLLKFGTEDSFMIGTDYSHTDISANLSGLDEVRSWVD
                     EGRITQAQGHKILETNSQAFYGL"
     gene            complement(791..1318)
                     /locus_tag="VFY96_01895"
     CDS             complement(791..1318)
                     /locus_tag="VFY96_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX5605233.1"
                     /translation="MSTQKNSSGEVQQAIIQGAITREYRADAQQVIQRLNALRSTEIM
                     SYLQYMQHGYMAVSLLSPALKGEFQAHATQELDHANRLAERIQQLGGVPIYDPREIAA
                     KAAKVGVAPEQGATLTDMVVEDLMLERQQIEAYTVFIREIGDQDFVTRHMLIAILAET
                     EKHANELADYLKRST"
     gene            1782..2348
                     /locus_tag="VFY96_01900"
     CDS             1782..2348
                     /locus_tag="VFY96_01900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX5605234.1"
                     /translation="MTPAIRILGIAGSLRRESYNRAALRAASQLVPQDATLEVFELDG
                     ISGFNQDEEKNPPAKVVELKRRVRDAHAILIVTPEYNYSVPGVLKNAIDWASRPYGDS
                     AWAGKPVAIMGASVGAIGTARAQYHLRQTFVFLNMFPVNQPEVMIGNAAERFDSAGNL
                     KDETTKKLISQLLTNLVEWTRRIGAAPR"
     gene            2369..3268
                     /locus_tag="VFY96_01905"
     CDS             2369..3268
                     /locus_tag="VFY96_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5605235.1"
                     /translation="MKPALTIESKVPLNDGRLIPQLGLGVWQTRAGATCEAAVLTALE
                     AGYRHIDTASMYGNEESVGAAIRKSGIPREQIFVTTKLWNSDHGNPQRALETSLRKLQ
                     VAYVDLYLIHYPVRQRIQSWHALELLRERGKARSIGVSNFTIKHLTELLQETKTIPAV
                     NQVEFHPYLYQRELLEFCRRSGIVLEAYSPLTKGERLKDRKLVAIANKYPTSPREGAN
                     SRSLPSILGKLPGHSNSKSTAQILIRWALQHGLVVIPKSANPKRILENADVFDFEISP
                     ADMELLDGFNEDLRTCWDPTDAP"
     gene            3446..>3799
                     /locus_tag="VFY96_01910"
     CDS             3446..>3799
                     /locus_tag="VFY96_01910"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5605236.1"
                     /translation="MTPSLARQRYYPWVVIGIAFLTVAVAFGTRNAFAVFLIAVIDEF
                     HWSRALASGALMLGSVLWTLAAPVIGIALDRFGPRLVVPTGAIIMALGFVISALAHSA
                     IEYYIGMGVLMGVGFA"
BASE COUNT          865 a          999 c         1058 g          877 t
ORIGIN      
        1 gagtgggcgc tgaccactac ctataaccga tggatggcag aaaaatgcgc aagcaccaac
       61 ggccgcctgc gctgggcggc ggtgcttcca ctgctgcagc ccgataaggc ggtcgaggag
      121 ctacggtggg ccaaggagca cggggcttgc ggtatttaca aacggggatt cgatctcgac
      181 aggaacatca ccgatcggca tttctttccc gtgtacgagg aagcgagcaa actcgatatt
      241 ccactctgca ttcataccgg ccacccactg cctttccacg aatgggaccg cggctttccg
      301 atcatgtatt cgtttagcgc cctggtgagc tccggcgtgg caaaaaaatt tcccagactg
      361 cgcttcggat tgatcgaatc gggcgcttcc tggattcctt atacgatttc gcagctggga
      421 gcggtaaaac gtcaatcgct tcgcggcaag ggaacgcgac tgccgcgtct ctacgaatta
      481 gagccggaaa ttttccgaca aaaccggatc tatatcacta tcgaccccat cgacgacatc
      541 gagtcgctgc tcaaattcgg cacggaagac agcttcatga tcggcaccga ttacagccac
      601 acggatattt ccgcgaacct gagcggactc gatgaagtgc gcagctgggt ggacgagggc
      661 agaattaccc aggcgcaagg acataagatt ctggaaacca attcgcaggc gttctacggc
      721 ctgtgaaccg ccatgggcgg tttacatgac cgcccttcac gcgctccaat gagttcagcg
      781 gcgctgccga ttatgtgctg cgcttgagat agtctgccaa ttcattggca tgtttctccg
      841 tctcggcgag gatagcgata agcatatgcc gggtgacaaa atcttggtcg cctatttcac
      901 gaatgaaaac ggtataggct tcgatttgct gtcgttccag catgagatct tcgacgacca
      961 tgtcagtaag cgtagcgcct tgctcgggcg caactcctac cttcgcggct ttcgcggcga
     1021 tttccctggg atcataaatg ggtacgcccc cgagttgttg gattcgttca gccagccgat
     1081 tggcatgatc taattcttgg gtagcatggg cctggaactc gcctttcaac gccggtgaaa
     1141 gtagtgacac cgccatgtag ccatgttgca tatactgaag atagctcatg atctctgtgg
     1201 aacgcagagc gttcaggcgc tggatcactt gctgcgcatc ggctctgtat tcccgtgtga
     1261 tagctccctg gataatcgcc tgttgtactt ctccagaact atttttttgc gtggacataa
     1321 ttcccccttc cctgcgagaa aaaataactg tacgacacgc tctgactgcc gtgttctcgc
     1381 gcaaaaatag taccaatttg atcaccttaa atttgttggg aaaagacaaa tgactccagg
     1441 gtcttggtga ttcttaagat tggtttaaag acgcacagga gaattgccga gcttgcagtg
     1501 tctgattttt gccgccagtt gggaaaatca ccttcagatg ttcgcaaagt tcggtttgaa
     1561 tgggcggaga ttttcggcgc ccgttgagct caatgttttt agcctccgca tactcgggac
     1621 aattttccca gcacaacttg attggcccgc ccccaggtat acgctcgaat gaatagctat
     1681 ttctttcaaa tcgttcgata gggcgcgcac tcttccaggc gtgccgctct cgaagatagc
     1741 gatgttgacg agtcttaaag ttttcgattt atggaggagc aatgacgcca gccatccgca
     1801 ttctcggaat cgccggcagc ttacggcgcg agtcctacaa tcgcgcggcg cttagagccg
     1861 caagccagct tgtgccacaa gacgcaactc tcgaagtctt tgagctggac ggcatatcgg
     1921 ggttcaatca ggacgaggaa aaaaatccgc cagcgaaggt ggtcgagctg aaacgccggg
     1981 tgcgcgatgc ccatgcgatc ttgatcgtga cgccggaata taactattca gttccgggcg
     2041 tgctcaagaa cgcaatcgat tgggcctcga gaccttacgg tgatagtgca tgggcgggga
     2101 aaccggtggc gatcatgggc gcttcagttg gcgcgatcgg cacggcgcgg gcgcagtatc
     2161 atctccggca gacttttgtt tttctcaaca tgtttccggt gaatcagccg gaagtgatga
     2221 tcggcaatgc cgccgaaagg ttcgactcag cgggaaatct caaggacgag acgacgaaaa
     2281 aattgatcag ccagctgctc accaatttag tcgagtggac gcggcgcatc ggcgcagcac
     2341 cacgataaaa aatgcttttc gtagagccat gaagccggcc ctgacgatcg aaagcaaagt
     2401 tcctctgaac gatgggcggc tgattcctca gctcgggttg ggcgtgtggc agactcgcgc
     2461 gggggcaact tgcgaggcag cggtgctgac agctctcgag gcgggttacc gccacatcga
     2521 cacggcatcg atgtacggca acgaagagag cgtgggggcc gcgatacgca agagcggtat
     2581 tccccgtgag caaatcttcg tcacgacaaa actgtggaac tccgatcatg gaaacccgca
     2641 gcgagcgctg gagacgagcc tacgtaaact gcaagtggcc tacgtggatc tttatttgat
     2701 tcattatccc gtgcgacaac gcattcagag ctggcacgcg ttggaactgc tccgcgaacg
     2761 aggcaaagcg cgttcgatcg gcgtaagcaa tttcaccatc aagcacttga cggagttact
     2821 tcaagaaact aagacgattc ccgccgtcaa tcaggtcgag tttcatccat atctgtatca
     2881 gcgggaactg ctcgagttct gccgccgcag cggcattgtc ctcgaggctt atagcccgtt
     2941 gactaaagga gagcggttaa aggaccggaa acttgtcgcg attgctaaca aatacccaac
     3001 atcgccgcga gagggcgcga attcgcggtc gctgccatca atcttgggaa aacttcccgg
     3061 ccactcgaat agtaaatcga cagcacagat cctgattcgt tgggcgctac aacacgggct
     3121 ggtcgtgatt cccaagtcag ccaatcccaa gcgcatcttg gaaaatgccg atgttttcga
     3181 tttcgagatc agtccagcgg atatggagct tctcgatggc ttcaatgaag atttgcgaac
     3241 ttgctgggat ccgaccgatg ctccgtaact aatttgtcag ccacgcgttt ccagcgaaat
     3301 ctctttcgat aacgcacgcg cgttcaaatc acggcgtctc gaaacagcca ccgattcatg
     3361 agaggaatgg gccagacatt gactcccgat gcgccgggtt ccgggagtag cgatgagttt
     3421 tgttgtgtcg atacaagaaa tagaaatgac ccctagcctt gcgcgccagc gctattatcc
     3481 atgggtggtt atcggcatcg cgtttttgac cgtggcggtg gcgttcggga cgcgcaatgc
     3541 atttgcggtt tttctgatag cggtcatcga cgagttccat tggagccgag cgctggcatc
     3601 cggcgcgctg atgctcggct cagtactgtg gactctagcg gcgccggtga ttggaatcgc
     3661 gcttgatcgg ttcggaccac gcctcgttgt tcccactggc gcaatcatta tggcgctcgg
     3721 gtttgtgatc agcgcattgg cgcacagcgc gattgaatac tacatcggca tgggcgtttt
     3781 gatgggcgtt ggttttgcg
//
LOCUS       DASULZ010000095         4294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1330015, whole genome shotgun sequence.
ACCESSION   DASULZ010000095 DASULZ010000000
VERSION     DASULZ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330015"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(244..1284)
                     /locus_tag="VFY96_01915"
     CDS             complement(244..1284)
                     /locus_tag="VFY96_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5605237.1"
                     /translation="MLLPFREHLDFGPTPYDLLQAKEGIPIIKGNVVADVNALKLGRW
                     ERTGAYGCYLNLSNQQQTDAYVCEIPPGSETLPQRHLFEEIIYVAHGRGATSVWQDGA
                     GKVTFEWSRGAIFAIPLNCSYVHYNGSRDEPVRLFGGTTCPRMINIFHNEEFIFRNPF
                     NFRDRFHEGEDFLRYNKHMADRYWETNLVPDVNAFPLDDFPMKGQGVKHMRFTLADTT
                     YGCHVSEFPSGSRSTFHRHGPGAVIIITHGEGYVMLWKDGEPRERHEFQVGTVYSPDD
                     LMWHGHFNTGKSTMRHFAMRGDSPKYSHDRFRNPLWTMIPMADEPPEIHREYVHILRE
                     KGVQTAVSVVED"
     gene            1402..1782
                     /locus_tag="VFY96_01920"
     CDS             1402..1782
                     /locus_tag="VFY96_01920"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEX5605238.1"
                     /translation="MKREVPCGITRTQTYATTLDMRARQLKADVFSTPAMIGLMERTC
                     VDMTEPYLDTDEQTVGIHVDVRHRAPTKIGQTVTVTAKLLEVKDDKILYAVSASNDQG
                     VKIGDGTHRRAVINTKRFGNGGVI"
     gene            1838..2011
                     /locus_tag="VFY96_01925"
                     /pseudo
     CDS             1838..2011
                     /locus_tag="VFY96_01925"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
     gene            2048..2359
                     /locus_tag="VFY96_01930"
     CDS             2048..2359
                     /locus_tag="VFY96_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEX5605239.1"
                     /translation="MQRARARIWIDYCNTRLQQAAGYIAHDHEVDKSKEKLREYLRTL
                     NQEMRDREYIAGDYSLADITYIPFFCRLERYQTVIGNDLPHVKAWMQRLLDRPAVRST
                     S"
     gene            complement(2408..2569)
                     /locus_tag="VFY96_01935"
     CDS             complement(2408..2569)
                     /locus_tag="VFY96_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605240.1"
                     /translation="MKGVPIAILALAFAFFSLLSLLTATRESDNRSAASADGPPRVSE
                     VGLRVTFQH"
     gene            3054..3896
                     /locus_tag="VFY96_01940"
     CDS             3054..3896
                     /locus_tag="VFY96_01940"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605241.1"
                     /translation="MASVWMAKEIGAFAKEALDIEVLSMSSSSAIPALIANEIDVVQV
                     SAAPVITASLRGIDVVFIAGLLNTMIWDFYTRPEIKTSEQLKGKIIGTDRPATPVAYG
                     TLVALKKMGLTPKDVQLRPLGSSPQIVAAFYAGQIAGGVGSPPASFLLERAGFHSLVS
                     LLDVPYQNVGLVVRRSRMEELGSRLVSLLRAVRAGIERYYADKPFAMKVIGRYAKETE
                     PEVLDRSYEFYKKAGFRRELLTSEPGLQGILDFLAESIPEAKTAKPAQFFDDRFVRQV
                     NSGK"
     gene            complement(4044..>4294)
                     /locus_tag="VFY96_01945"
     CDS             complement(4044..>4294)
                     /locus_tag="VFY96_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605242.1"
                     /translation="MGAAVAKADTKAEHAGCSTSQVASLFLLAGSPEALAHNRKAVWV
                     KAGSFERVIVATSFPRRRYKNRSINFGSCHFLQQLLAC"
BASE COUNT          983 a         1198 c         1161 g          952 t
ORIGIN      
        1 tcgcatccca cggttgatcg cgggtccgta gcgccgtcga gcgcttgtcc cagggcgcct
       61 cgagatactt tcgaatgtaa cgctcttttt ccaagacatg cccgtcggca tcgatcactg
      121 gacaattaac cataggacct cctgctgcgt tcgagatggt tcacacagaa gcgccgagta
      181 acacagcgaa agataatttt gcctccgtgt cccctgcggc tccccagcga tagtttcttt
      241 ttttcaatcc tccacgacgg agactgcggt ttgcacgcct ttttcgcgga ggatgtgcac
      301 gtactctcta tgaatttccg gcggctcgtc tgccatggga atcatcgtcc aaagcggatt
      361 tctaaatcga tcgtggctgt attttggact gtcgccgcgc atcgcaaagt ggcgcatcgt
      421 cgacttgccg gtattgaaat ggccgtgcca catcagatcg tcaggggaat aaacggttcc
      481 tacttgaaac tcgtgccgct cgcgtggctc accatccttc caaagcatca cgtaaccttc
      541 gccgtgggtg ataatgatga ccgcgcccgg tccatggcga tgaaacgtgc tgcgggaacc
      601 ggacggaaat tccgacacat ggcagccata ggtcgtgtcc gctaatgtga agcgcatgtg
      661 cttaacgccc tggcccttca tagggaaatc gtcgagcgga aatgcgttca catccggcac
      721 cagattcgtt tcccaatagc gatccgccat gtgcttgttg tagcgcagga agtcctcccc
      781 ttcgtgaaag cgatcacgaa agttgaaggg attgcgaaaa ataaactcct cgttgtgaaa
      841 gatgttgatc atgcgcgggc aggtggtacc gccgaacagc cgcaccggct catcccgcga
      901 accgttatag tgaacgtagg aacaattcag cggtatcgcg aagatcgccc cgcgagacca
      961 ttcgaacgtc actttacctg cgccgtcttg ccagacggag gtcgcgccgc gtccatgggc
     1021 gacataaata atttcttcga acagatggcg ctggggcaac gtctcggaac ccggaggaat
     1081 ctcgcacacg taggcgtccg tctgctgttg gttggagaga ttgagataac agccgtaggc
     1141 accggtgcgc tcccagcggc cgagcttgag agcgttcaca tcggccacga cattgccttt
     1201 gatgatggga atgccttctt tcgcttgcaa cagatcgtag ggcgtagggc caaagtcgag
     1261 gtgctcacga aacggcaaca acattattcc aaactccttc gggatgcgta tctcatagca
     1321 cgaattcgcg cggtgatcac aggttcgacc aattcattgt cgctattgcg catccgccat
     1381 aggctctcgt atagtcttcc tatgaagcgc gaggtaccct gcggcataac ccgaacgcaa
     1441 acctacgcga ccacactcga catgcgcgca cgccaattga aagccgatgt tttttccacc
     1501 cccgcgatga tcggcttgat ggaacggacc tgcgttgata tgacggaacc ctatttggac
     1561 actgacgaac aaaccgtcgg aattcacgtt gatgtgcgcc atcgtgcccc gacgaaaatc
     1621 ggtcaaaccg tcacagtgac ggccaaactg ttggaagtga aggacgataa aattctttac
     1681 gcagtttccg ccagcaacga tcagggtgtc aagatcggcg acggaacgca ccgtcgcgcg
     1741 gtgatcaaca caaaacgatt cggcaatggc ggggtgattt aaagaaggga gagttacaat
     1801 gaaactttat tcctaccagc cttgacccta cgctcacagg acaaggattg tcctagcgga
     1861 aaaaagactt tcctacgagc tgatcgaatg tgaccttagc aacaagccga aagatctttt
     1921 ggagctaaac ccctacggaa aagtccccgt gctcgtcgat ggcgacggag tgatttacga
     1981 gtcggcgatc atcaatgaat atctcgaaga aagatatcct ggtgttccgc ttttgcccaa
     2041 ggacccattg cagcgcgcac gcgcacgtat ctggatcgac tactgcaaca cgcgcctaca
     2101 acaagcggcg ggctatatcg cccacgatca tgaagtcgat aagtcgaagg aaaagctgcg
     2161 cgaatatctg cgcactctga atcaggagat gcgcgaccgc gagtatattg cgggagatta
     2221 ctcgctcgcc gacattactt acatcccgtt tttctgtcgg ctagagcggt atcagacagt
     2281 catcgggaac gatttaccgc acgtaaaagc ctggatgcag cgcttgctcg accggccggc
     2341 ggtgcgctca acgtcgtagc atcaccggct tgagaccgcc gcgggagaag aatcattggc
     2401 gcgattttta gtgctgaaaa gtgacgcgga gcccgacttc ggaaaccctg ggcggtccat
     2461 cggccgacgc cgcacttctg ttgtccgact cacgcgtggc agtgagcagt gacagcaacg
     2521 aaaagaacgc gaaagcaagc gccagtatgg cgatcggtac acctttcata cccgcctcct
     2581 aggccgagga catcgcggca gacatgtggt agacgagcga gtcgcgtgat cgtttacagt
     2641 gtctcctgcc gataaactac gactatcacc tagcaagcgc agcgcaaggc ccgtaccggt
     2701 ctgcacaggc aaaagccacc gcttttgacg cggatctcga ccgaacttcg ccaattagca
     2761 gcacggaatt gtgcggtttg tagcggaagc gcttgtcacc ggatgtggtt cgtcccgctt
     2821 gacgccagag aaagctctgc cctattttta ctaaggcact agttgcatgt tttttactgt
     2881 gaggtccttg catgtcgctt ctcatgctct gttatgtagg tcgaggttcg gttgcgcttg
     2941 gcgcgtccgt ccttgtcgct ctgatttcgt ttgttaaccc gatagcaagc tgcgcgcaaa
     3001 cccgggcgcg cgcacgacca ctacgcgtcg cttatctcag cacatctgcg acaatggctt
     3061 cggtttggat ggccaaggag atcggcgcct tcgcgaagga agccttagac attgaggtcc
     3121 tgtcgatgtc gtcgagcagc gcgattcccg ctttgattgc aaatgaaatc gatgttgtgc
     3181 aggtctccgc tgcgccggtg attaccgcct ccctgcgcgg catcgacgtg gtattcatcg
     3241 ccggattgct caatacgatg atatgggatt tttacactcg cccggaaatc aagaccagcg
     3301 aacaactgaa aggcaaaatt atcggcaccg accggccagc tactcccgtc gcctacggca
     3361 ctctcgtggc gctgaagaaa atggggctga cgccgaagga tgtccaactc cggccgctgg
     3421 gcagcagtcc gcaaatcgtc gctgcttttt atgcaggcca gattgccgga ggggtcggct
     3481 cgccgcccgc gagttttcta ctcgagcggg ccggttttca ctccctggtc agtttgctcg
     3541 acgtcccgta tcaaaatgtc ggtctcgtgg tgaggcgcag ccgtatggaa gagctcggtt
     3601 cccgcttggt ttcgctgttg cgagcggttc gagctggaat cgagcgctac tacgcggaca
     3661 agcccttcgc catgaaagtg atcggcaggt atgccaagga aactgaacca gaagtgctcg
     3721 accgaagtta cgagttttat aaaaaagccg gtttccgtcg cgagttgctc acatccgaac
     3781 cgggacttca gggcattttg gattttctcg cggaatcaat tccggaagcg aaaaccgcca
     3841 agcccgcaca attctttgac gatcgcttcg tacgccaggt caacagcgga aaatgacgaa
     3901 agttcttgac gattggctgc cacatgactc cttcgacggt ttttagatga cgatcattga
     3961 cgcccaagtc cacatctggc gctctgaaag accaaacggt tgtacgtcac agaagatgca
     4021 tccaaaccgc atcggcccat cccttaacac gcgagcagtt gttggagaaa atggcagctg
     4081 ccgaagttga tcgagcgatt cttgtaccgc cttcttggga aggatgtcgc aacgattacg
     4141 cgctcgaagc tgcccgcctt tacccagacc gctttgcggt tatgggcaag tgcctctggt
     4201 gatccggcca gtaaaaacaa actggccact tggctcgtcg agcaaccagc atgctcggct
     4261 ttcgtatcag ctttcgcgac agcggcaccc attc
//
LOCUS       DASULZ010000096         2663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_416902, whole genome shotgun sequence.
ACCESSION   DASULZ010000096 DASULZ010000000
VERSION     DASULZ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416902"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VFY96_01950"
     CDS             complement(<1..650)
                     /locus_tag="VFY96_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605243.1"
                     /translation="MRQDYIIDPTRREFLKAASLCASWALFKLPSEAPAAEPALETTR
                     LKLVRTTSICQAPQYVGEELLKAEGFTEVTYVPKASPGEIGKALASGEADINLHFAGP
                     LIMSLDAGDPIVVLSGNHIGCFELFGTDKVRAIRDLKGKTVAVPALGSPPYVFLSIIA
                     SHVGLDPRKDINWITRPTAESMQMLAESKIDAYLGFPPEPQELRAKKIGHVLVNSV"
     gene            complement(647..2203)
                     /locus_tag="VFY96_01955"
     CDS             complement(647..2203)
                     /locus_tag="VFY96_01955"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5605244.1"
                     /translation="MSRGLLAWLPRLIAHVPVKVHAKLLAAFLAMVLLLIAVGAVGLR
                     ALSEANGRAAELVGLQRKIAAYRQLQHNTTAQLYSVASALLVHDEATLDATLRQLNQF
                     GYDFDRLQFVAKDEVELLRQVRKDYEEFIKVVSQAIELIRGGRIAEGNQLQVTEANPL
                     AERLERLMNQLVNRAESDMVASIDASQAGYAHSQGFVIVFAVMSIALAIVLGYAISWS
                     IVGPVQEMGARFKQLASGDFSQRIEIPNRDELGNLAINLNRMSQELAQLYQEIEEKNE
                     QLTAANKHKSDFLARVSHDLRTPLNSIIGFTRIVLRRMEGQLPSLQKENLQKVLISSE
                     HLLNLINELLDLAKIDAGKMEVIAATFRVEDVINMTTATVEPLLKGGCVRLVKDIAAD
                     LPLVRTDRDKLKQILFNLLSNAAKFTEQGEIRVSAARENGTLKLAVSDTGIGMTREAL
                     EHIFEEFQQAEKTTASRYGGTGLGLAIVKKFINLMGGEIVVESEIGKGSKFTITMPIE
                     LRSQAEERSA"
     gene            complement(2200..>2663)
                     /locus_tag="VFY96_01960"
     CDS             complement(2200..>2663)
                     /locus_tag="VFY96_01960"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX5605245.1"
                     /translation="HGKRLSLNDLRGKVIAISFIFASCADTCPLLTAKMVFLQNKLGS
                     AFGPEVRFLSVTVDPERDTPEVLKHYAAAYHANARGWAFLTGTKAEIREVAKRYGIYY
                     KKTPRGDVDHTFLTSLVDRHGNLRVQYMGVRFNPDEMLRDIQSLVAEAKRT"
BASE COUNT          580 a          739 c          714 g          630 t
ORIGIN      
        1 atgacgctgt tcaccaacac atgaccgatc ttcttggctc gcagttcctg aggttccggc
       61 gggaatccga ggtaggcgtc gatcttcgac tcggcgagca tttgcatcga ttcggccgtc
      121 gggcgggtaa tccaattgat atctttacgg ggatcgagac cgacatggga cgcaataatc
      181 gagagaaaaa cataaggggg cgagccgagc gccggcaccg caaccgtttt tccttttaaa
      241 tcgcgaatcg cccgcacttt gtcggtgcca aataattcga agcaaccaat atgatttccc
      301 gatagaacca caattgggtc gcccgcgtcc aaagacatga tcagcgggcc ggcgaagtgt
      361 aagttgatat ccgcttcacc ggaggctagg gctttaccga tttcgcccgg cgatgccttt
      421 ggaacgtatg ttacttctgt aaatccctcg gctttgagca attcctcgcc aacatattgg
      481 ggagcctgac aaatactcgt cgtccgcact aattttaacc gggtcgtctc taacgccggc
      541 tctgcagcag gcgcttcaga aggtagctta aagagtgccc aactcgcaca gagcgaagcc
      601 gcctttagaa attccctgcg cgtcggatca attatgtagt cctgtctcat gccgacctct
      661 cttccgcctg cgatctcaat tcaatcggca tagtgatcgt gaacttggag cctttgccga
      721 tctcgctttc gacgacgatc tcgccaccca taagattgat aaatttcttg acgatcgcca
      781 atccgaggcc ggtgccgccg tacctgcttg cagtggtctt ctcggcctgc tgaaactcct
      841 cgaaaatatg ttccaacgct tcgcgtgtca tgccaatgcc cgtatccgac acggccaatt
      901 tcaacgtgcc gttctcgcgt gccgccgaca cgcgaatctc accttgttcg gtaaactttg
      961 ccgcattgct cagcaaattg aacaagatct gcttgagctt gtcgcggtcg gttctgacta
     1021 agggaagatc agccgcgata tccttgacca gacggacgca tccgcctttg aggaggggct
     1081 cgacggtagc cgtcgtcatg ttaatcacat cttccactcg gaaggtagcg gcgatcactt
     1141 ccattttgcc cgcgtcgatc ttggcaaggt cgagaagctc gttgatgagg ttaagcaagt
     1201 gttccgagct gatcagaact ttctgcagat tctctttctg caggctgggc agttgccctt
     1261 ccatacgacg cagtacaatg cgagtaaagc cgatgatcga attgagaggc gtgcgcagat
     1321 cgtggctcac gcgagcgaga aagtccgact tgtgcttgtt ggcagcggtg agctgttcgt
     1381 ttttttcttc tatttcttga tacagctgtg cgagttcttg gctcatgcga tttaagttga
     1441 tggcgagatt ccccagctcg tcacgattag gaatttcgat gcgctgggaa aaatcgcccg
     1501 atgcgagttg cttaaaacgt gcgcccatct cctggacagg gccgacaatt gaccacgaga
     1561 tggcgtagcc gagaacgata gccaaggcga tgctcatcac ggcaaaaaca attacgaatc
     1621 cttgcgagtg tgcgtacccc gcttggctgg catcgatgct ggcgaccatg tcggattcgg
     1681 cgcgattgac cagttggttc atcaagcgct cgagccgctc ggccagtgga ttggcttctg
     1741 taacttgcag ctggtttcct tctgcaatcc tgccgccacg gatgagttcg atggcttggc
     1801 tgactacttt gataaattcc tcgtagtctt tgcgtacttg gcggagcagt tcgacttcgt
     1861 ctttagcgac gaactgcagc cgatcgaaat cgtaaccgaa ctgattgagc tgacgcaacg
     1921 tggcgtcgag agttgcctca tcgtggacca agagtgctga ggccacgcta tagagctgtg
     1981 ccgttgtgtt gtgctgaagt tgtcgatagg cggcgatttt tcgctgaagg ccaaccaact
     2041 cggcggcgcg gccgttggct tcactcaaag cccgcaggcc gaccgcgccc accgcgatca
     2101 gcagcagcac catcgcgaga aaggccgcca gcaacttggc gtgcactttc accggcacgt
     2161 gcgcaatcag ccggggcaac caggctagca acccgcgact cacgtccttt ttgcctccgc
     2221 cacaaggctc tgaatgtcgc gaagcatctc gtcaggattg aatcggacac ccatgtactg
     2281 cacgcgcagg ttcccgtgcc gatcgactag cgatgtcaga aaagtgtgat cgacgtcgcc
     2341 gcgcggcgtt ttcttgtagt agatgccgta gcgtttggcg acctcgcgga tctcagcctt
     2401 tgtgcccgtt aggaaagccc acccccgggc attcgcgtga tacgctgcag catagtgctt
     2461 gagaacctcc ggcgtgtccc gctccgggtc tacggtgact gaaagaaatc taacttccgg
     2521 accaaaggcc gagcccagct tattctgaag aaaaaccatt ttcgccgtaa ggagcggaca
     2581 agtgtcagcg caactggcaa agataaagct aatcgcgatc accttgccac gaagatcatt
     2641 taacgagaga cgtttaccgt gtt
//
LOCUS       DASULZ010000097         7639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_721148, whole genome shotgun sequence.
ACCESSION   DASULZ010000097 DASULZ010000000
VERSION     DASULZ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7639
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721148"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..1511
                     /locus_tag="VFY96_01965"
     CDS             183..1511
                     /locus_tag="VFY96_01965"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5605246.1"
                     /translation="MDNESSENIQKRPPLSLPFYYGWFVISLCFLTTLTSAGVRSSPS
                     VLIHPLEAEFGWSRTLIASAVSMNLLLFGIAAPLSGFLIDRFGPRKVMMGSLGLLIAG
                     VSGTIVMTQFWQFFLVWGVIVGLGAGGVGSVLTATVGNRWFIARRGLALGILGSASST
                     GQIIFLPLFMAMITYAGWRLGSMALIIVAIVLLPLIFLFMRDDPSEVGLEPYGAGDPK
                     IASNTSAASLRGMPAKNATITAREVIGHPTFWLLAGSFFVCGGTANGLIGTHLIPHEI
                     DIGIPQIAAASLLGIMGGLNMVGTIFSGWMVDRVRANRWLALVYAMRGVSLLILPFVK
                     DFSGLVFFAIIYGLDWFATVPPSMAITADTFGRQNVGKVYGWIFMSHQIGAAIMASTA
                     GAIRTWMGDYQFAFLSGGVIAMIAAGLALQIKLKPKEAITPPAGAVPAGA"
     gene            complement(1776..2243)
                     /locus_tag="VFY96_01970"
     CDS             complement(1776..2243)
                     /locus_tag="VFY96_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605247.1"
                     /translation="MSAASVGRVVYPNMLINFAMAKSFSLLEAAFLALILTASACGRD
                     LNSAQGVAEEFVDQHYVVIDLPKAKTLAVSVALSKLNEEIRLTSGQPIDASTRKPRVH
                     YTLLEKKEGDQRATFLYQGTIQSDDNTSFTRQWLITARREGTQWRVSNFTESD"
     gene            2287..3195
                     /gene="xerC"
                     /locus_tag="VFY96_01975"
     CDS             2287..3195
                     /gene="xerC"
                     /locus_tag="VFY96_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEX5605248.1"
                     /translation="MEKLIEQYRAHLRNERNVSPHTLRNYLSDLHQFHQFVSERNWAV
                     DSTGCVDVRKIEIQVVRAFLASLTPGRKKSSLGRKIACLKGFFKYLVTTRQIEGDPMF
                     SIHSPKNEKPLPAFLSVDDVFQLLGAVKTKTELDVRDRAVLEFFYSTGVRVSELVGLN
                     WADVDFRLEIVRVTGKGSKERIVPIGEIALEALQDYAREQRKRWNFSCKGETAVFLNN
                     RGRRITTRSVARIVEKHLKQAGIHVKMGPHGLRHSFATHLLNSGADLRSIQELLGHAS
                     LSTTQRYTHLNLDQLTAVYDKAHPRA"
     gene            3263..3793
                     /gene="hslV"
                     /locus_tag="VFY96_01980"
     CDS             3263..3793
                     /gene="hslV"
                     /locus_tag="VFY96_01980"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEX5605249.1"
                     /translation="MIHGTTILAIRRDGKVVVVGDGQVSMGQTVMKRGARKVRKLHHD
                     RVIAGFAGATADAFTLFEKFEAKLEQFNGNLKRAAVELAKDWRTDRVLRRLEALMVIA
                     DSDDLLVISGAGDVIEPDDNVIAIGSGGNYALAAARVLLKHTQLDARLIAEEAMKVAA
                     GICVYTNEQLTFEELP"
     gene            3976..5358
                     /gene="hslU"
                     /locus_tag="VFY96_01985"
     CDS             3976..5358
                     /gene="hslU"
                     /locus_tag="VFY96_01985"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006424976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEX5605250.1"
                     /translation="MTDGVKTNLPESGAPAPVMTPREIVSELDRYIVGQNEAKRAVAI
                     ALRNRWRRQLVPEDLREEIAPKNIIMIGPTGVGKTEISRRLAKLSQAPFIKVEASKFT
                     EVGYVGRDVESIIRDLTDLAVNMVKEEEKQKVEIKAREVAEERVLDLLLPASAGETDG
                     DAVDAARLQSTREKLKKMLREGKMDDRFVDIELTQSAMPMIEVLTPQGMEGMEFNLKE
                     MFSNLMPKKTKKKTVKIPEALEILTQEEASKLVDMDKVTGEAIRRVEQSGIVFLDEID
                     KIAGRESMQGPDVSRQGVQRDLLPIVEGSTVNTKYGMVKTDHILFIASGAFHTAKPSD
                     LIPEFQGRFPIRVELSSLSKADFVRILTEPKNALTKQYEALLETEGVHLRFAEDAIQE
                     IALIASDVNERTENIGARRLHTILERLLDEISFTAPETHGREVAIDARYVRERLAPIL
                     KDEDLSRYIL"
     gene            5477..6361
                     /gene="argB"
                     /locus_tag="VFY96_01990"
     CDS             5477..6361
                     /gene="argB"
                     /locus_tag="VFY96_01990"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX5605251.1"
                     /translation="MENFIGKAEVLMEALPYIQRFYGKTFVIKYGGHAMENEELKTSF
                     AQDIVLLKYVGINPVVVHGGGPQIDSALDKMGITSRYVRGMRVTDKETLDIVEMVLVG
                     KVNKEIVTMINQHGGMAVGLSGKDGGLILAKKMSVTVESSNGEAPETLDIGMVGEIVG
                     INPLVIESLDQNKFIPVIAPVGVGRDGETYNINADLVAGEIAEALHAEKLILMTDVEG
                     VKDKKSTLLSTLKENQARKMIQDGIVGAGMIPKVECCINALKGGVEKTHIIDGRVKHA
                     VLLEIFTREGVGTEVVRK"
     gene            6361..7551
                     /locus_tag="VFY96_01995"
     CDS             6361..7551
                     /locus_tag="VFY96_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX5605252.1"
                     /translation="MKNREVAALTEKYVARTYARAPIALVKGRGIKVWDADGKEYLDF
                     LAGIAVNSLGHCNPAIVKAIKGQAQQLLHVSNLYHIQPQAELARELCRHSFADRAFFC
                     NSGAEANEAAIKLARRYGLEKRAGKFEILSTHNSFHGRTLATLTATGQEKIRAGYDPL
                     PVGFRQVPYNDLNATEAAIDGQKTVAILVEPIQAEGGVVVANEIYMLGLREICDQRGL
                     LLIFDEVQTGMGRTGKLFGYEHFGVKPDIMTLAKALGGGLPLGAMLAREEIAASFVAG
                     SHASTFGGNPVSCAAGLAVMNALIKGGVLKNCVQMGKYFVKGLEALKKRFSFIREIRG
                     KGLLLGLELTIEGNKIAEVCMQEGLLINCTASKVLRFAPPLTITKREIDRGLAILENV
                     LARQ"
BASE COUNT         1757 a         1960 c         2221 g         1701 t
ORIGIN      
        1 ccgcccgcag ttgcagagga atcaggagca cgcagcaaac gatgatcatt cgaggcggct
       61 ggttcatcca gcgatagata acatgcagag atctacccaa caattcccct ccggccaaac
      121 atctaacaga caaacttgca cccaggtttg tgctttccgc aaaaggtcag tataaaaaat
      181 agatggacaa cgagagttca gaaaatatcc aaaagcgccc gcccttgtca ttgcctttct
      241 actacgggtg gttcgttatt tccctttgct ttctaaccac tttgaccagt gccggcgtgc
      301 gttcgtcgcc ctcggtgctg atccacccgc tggaagctga gttcggttgg agccgcactc
      361 tgatcgcttc agcggtcagc atgaatcttt tgctcttcgg catcgctgca cccctgagcg
      421 gttttttgat cgaccgtttc gggccgcgca aagtcatgat gggaagtctg ggcctcctca
      481 tcgccggtgt cagcggcacc atcgtcatga ctcagttctg gcaatttttt ttggtctggg
      541 gcgtgatcgt gggccttggg gctggaggtg tgggttcggt attgaccgca acggtcggca
      601 accgctggtt tattgcccgc cgtggtttgg ctctaggaat tctcggcagc gccagttcca
      661 cgggacaaat tatctttttg cccctgttta tggcgatgat tacctatgcc ggctggcgct
      721 taggctccat ggcattgatc attgtcgcga tcgtgttatt accgttgatc ttcttgttca
      781 tgcgtgacga cccctcagag gtcggtttgg aaccctacgg cgcaggtgac ccgaaaatcg
      841 ccagcaatac tagtgcggcg tcacttcgcg gcatgccggc gaaaaacgcc acaatcacgg
      901 cacgcgaagt gatcggtcat ccaactttct ggttgttggc cggcagtttt tttgtctgcg
      961 gtggaaccgc gaacggatta atcgggacgc accttatccc gcacgaaatc gacatcggca
     1021 ttccgcaaat cgctgcagcg tctttgcttg gtattatggg cggcttgaac atggtcggga
     1081 cgattttctc cggctggatg gtcgatcgag tcagagcgaa tcgttggctg gcgctggtct
     1141 atgccatgcg aggggtatcg ctattgatct tgccgttcgt aaaggatttt agcgggctgg
     1201 tcttttttgc gattatctat ggccttgact ggtttgccac cgtgccgccg tcgatggcga
     1261 ttactgccga tacattcggc aggcaaaacg tcggtaaagt ttatggctgg attttcatgt
     1321 cacaccagat cggcgccgcg attatggctt ccaccgcggg cgcgattcgc acctggatgg
     1381 gcgattatca gtttgcgttt ttgtccggcg gagtgatcgc gatgattgct gccggattgg
     1441 cgttacagat caagctgaaa cccaaggaag cgattacccc accagcggga gcggtgccgg
     1501 cgggagcctg agcagcacgc gggcgtcggt ctttcagaaa tttcgcagaa attttaccgc
     1561 agaggcagag agaacccata gagaaactag gacgcgttct ccgggtcctc ttatgaaatt
     1621 ccgattagag gcaaaaattc cccggtccta aggcccgccc cctctttgcc aaagcgggcc
     1681 atggagaatt tgtcagtatt ggccgaacca ctacggctca ttctcatgcg ctgtgttgta
     1741 ccgattggta catgaatgac tctgcggttt aacattcagt ccgattcagt gaaattggac
     1801 acgcgccact gggtgccttc cctgcgcgcg gtgatcaacc attggcgcgt gaacgacgta
     1861 ttgtcgtccg actgaattgt gccttggtag agaaacgtcg cgcgttggtc gccttccttt
     1921 ttttcgagaa gcgtgtaatg cacacgcggt ttgcgggttg aggcgtcgat tggctggccc
     1981 gaggtcaagc gaatttcctc gttcaatttg cttaaagcca cgcttacggc aagcgtcttc
     2041 gccttgggca aatcaataac gacatagtgc tgatcgacaa actcctcagc gactccttga
     2101 gcggagttga gatcccgacc acatgcgctg gcggtgagga taagagcaag aaacgctgct
     2161 tcaagcagcg aaaacgattt tgccatcgca aaattaatta gcatgttcgg gtagacaacg
     2221 cgacctactg aagcagcgct cattgttgtt ccataaccgg gcatgatatc ttggtcttcg
     2281 gctggaatgg aaaagctcat cgagcagtac cgcgcccacc ttcgtaacga acgtaacgtt
     2341 tcgccccata cgctaagaaa ttacctcagc gatttacatc agtttcacca gtttgtcagc
     2401 gagcgaaact gggcggtgga tagtacggga tgcgtggacg tgcgaaaaat cgagattcag
     2461 gtcgtgcgcg catttttagc atcacttacg cccgggcgaa aaaagagttc cctcggcaga
     2521 aagattgcct gcttgaaagg gttttttaaa tatctggtta cgacgcggca aatcgagggc
     2581 gatccgatgt tttcgattca ctccccaaaa aatgaaaagc cgctgcctgc atttctctcg
     2641 gtcgatgatg tcttccagct gctcggagcg gttaaaacca agactgagct tgacgtgcgc
     2701 gatcgcgccg ttctggagtt tttttattcc accggtgtcc gggtgagcga gcttgttgga
     2761 ctcaactggg ctgacgtcga ttttcgcctg gagatcgtgc gggttacggg taaaggttcc
     2821 aaagaacgaa ttgtgccgat cggcgaaatt gcgctcgagg cgttgcagga ttatgctcgg
     2881 gaacagcgca aacgctggaa tttttcctgc aaaggcgaaa ccgctgtgtt tctgaataat
     2941 cgcgggaggc gcatcacaac gcgaagcgtc gcccgaattg tggaaaagca tctgaagcag
     3001 gccggcattc atgtcaaaat gggaccgcac ggtttgcgac acagctttgc cacgcatcta
     3061 cttaacagcg gcgcagatct gcgctcgatt caggaactgc tcggacatgc gagcttgtca
     3121 acgactcaac gctacacgca cctgaatttg gatcagctaa ccgcagtgta cgacaaagcg
     3181 cacccgaggg cgtgagaaca tttttgattt tggattttag attttcgatt tggaatccga
     3241 aattggcaaa cgggaatcga aaatgattca cgggacgacg atattggcga ttcgacgaga
     3301 cggaaaagtc gtcgttgtcg gagacggcca ggtgagcatg ggtcaaacgg tgatgaaacg
     3361 cggcgcgcgc aaagtccgca agctgcacca tgaccgtgtc atcgccggat tcgccggagc
     3421 cacggcggat gcttttacct tgttcgaaaa attcgaggcc aagctcgaac agttcaatgg
     3481 caatcttaag cgtgccgcgg ttgagttggc aaaggactgg cgcacagatc gggtgttgcg
     3541 gcgcctggaa gcgctgatgg tcatcgcgga cagtgacgat ctgctggtga tctccggtgc
     3601 cggcgatgtc atcgaaccgg acgataacgt tatcgcgatc ggctccggag gcaattacgc
     3661 cttggcggcg gctcgagttc ttctcaagca cacgcagttg gatgcccggc tcatcgccga
     3721 agaagcgatg aaagtggcag ccggcatctg cgtctacact aacgaacagc ttacctttga
     3781 agaattgccg taggcactaa gaccgagacc gtgtcagttg cgcattcctt ttggcggctc
     3841 ggagcacgct aacaactcga gtactggagt cgggagtgat ggggtttaga cccagtactc
     3901 caatattcca gtagtcgaac ggccattccc aaatgattgc ttttggtggg gaaattgtta
     3961 ggagtaaata ttcccatgac agacggagta aaaactaacc tacccgaaag cggtgcaccc
     4021 gcgccggtaa tgacgccccg ggaaattgtt tcagagctcg atcggtatat cgttggtcag
     4081 aacgaagcga agcgggctgt ggcgatcgcg ctacgcaacc gatggcgccg ccaactggtg
     4141 cctgaggatc tacgagaaga gatcgcgcca aagaacatca tcatgatcgg tccaacggga
     4201 gtgggtaaaa cggaaatctc ccggcgctta gcgaaactct cacaggctcc ctttatcaaa
     4261 gtcgaagcgt ccaaatttac tgaggtcggc tatgtcggcc gggatgtcga gtcgatcatt
     4321 cgcgatttga ccgaccttgc ggtcaacatg gtgaaagagg aagagaagca aaaagttgaa
     4381 atcaaggcgc gcgaggtcgc cgaagaacgg gtgctcgatc tccttttgcc agcgagcgcc
     4441 ggcgagacgg acggcgatgc cgttgatgct gcccggttac agtcgacgag ggaaaagctc
     4501 aaaaaaatgt tgcgcgaagg caaaatggac gaccgtttcg tcgacatcga gctgactcaa
     4561 tcggccatgc cgatgatcga agttttgacg ccccagggca tggaaggaat ggagttcaac
     4621 ctcaaggaaa tgttttccaa cctgatgccc aagaagacca aaaaaaagac cgtgaaaata
     4681 cccgaggcgc tggagatcct tacgcaggaa gaagccagca agctggtcga tatggataaa
     4741 gtcacgggcg aagcgattcg ccgggtggag caatccggaa ttgtttttct agacgagatc
     4801 gacaagatcg ccgggcgaga atccatgcag ggccctgatg tttcgcgcca aggcgtacag
     4861 cgggatcttt tgccgatcgt cgagggttct accgtcaata ccaaatacgg catggtgaaa
     4921 actgatcaca ttctgttcat tgcctcgggt gcttttcata ccgccaagcc ctcggattta
     4981 attcctgagt tccagggacg tttcccgatt cgggtcgagc tcagttccct cagtaaagcg
     5041 gatttcgtgc ggattctgac cgagccgaag aatgcgctga ccaagcagta tgaagccttg
     5101 ctggaaaccg aaggcgttca tctgcgattt gccgaagacg cgatccagga aatcgcgctg
     5161 atcgccagcg acgtcaacga gcgcacggaa aatatcggtg cgcgccggtt gcataccatc
     5221 ctcgaacgct tgctggacga gatctctttt accgcgccgg aaacccatgg ccgggaagtc
     5281 gccatcgatg cgcgttacgt ccgcgagcgg ctcgcgccga tcttgaagga cgaagatcta
     5341 tcgcgctata tcctctgaga agcgcgggtg gatgctggag tgacagatgc tcggtgacgt
     5401 cgcccaagac tcctaccgtc cggtaacgca tcttttttag atattttgat gtgcgctttg
     5461 tgctaagctc cgagtgatgg aaaacttcat cggcaaggct gaggtcttga tggaggcctt
     5521 gccctacatt cagcgcttct acggcaagac cttcgtcatc aaatacggtg gtcatgcgat
     5581 ggaaaacgag gagctcaaaa cgagcttcgc gcaggacatc gtgttgctga aatatgtcgg
     5641 aatcaacccc gtggtggttc atggcggcgg gccgcaaatc gactcggcgc tcgacaaaat
     5701 gggcatcacg agccggtatg tccgcggcat gcgcgtcacc gacaaagaga cgctcgacat
     5761 cgtcgagatg gtcctggttg gaaaggtcaa caaagaaatc gtcacgatga tcaaccagca
     5821 cggcggcatg gcggtaggtc tcagcggcaa ggacggcggc cttatcttgg ctaaaaagat
     5881 gagcgttacc gtggaatcga gcaatggcga ggcgccggag acccttgata ttggcatggt
     5941 cggcgagatc gtcgggatca atccgctggt gatcgaatcc ctggatcaga ataagtttat
     6001 tccggtgatt gcgccggtcg gcgtcggtcg agatggagag acttacaaca tcaacgccga
     6061 tctggtcgcg ggcgaaattg ccgaggcgct gcatgccgaa aagctgattc tcatgacgga
     6121 cgtcgagggc gtcaaagaca aaaaaagcac cctgttgagc accctcaaag aaaatcaagc
     6181 gcgcaagatg atccaggacg ggatcgtggg cgcgggcatg attcccaaag tcgaatgttg
     6241 tatcaatgcg ctcaagggcg gtgtcgagaa aacgcacata atcgacggcc gggtgaagca
     6301 cgccgtgctg ttggaaattt ttacgcggga gggtgtgggg acggaggtgg tgcggaagtg
     6361 atgaagaacc gggaggtcgc ggctttaacc gaaaaatacg ttgcccggac ttatgcgcgc
     6421 gcgccgatcg ccctggtcaa aggacgcgga atcaaggttt gggatgccga cggcaaggaa
     6481 tatctcgatt ttcttgccgg gatcgcggtc aacagtcttg ggcactgcaa cccggcgatc
     6541 gtcaaagcga tcaaggggca agcccaacaa ctgctgcacg tttccaacct ctatcacatc
     6601 cagccgcagg cggagttggc ccgggagcta tgccgccatt cgtttgcgga tcgggcgttt
     6661 ttctgcaaca gcggagccga agcgaatgaa gcggccatca agctggcgcg ccgttacgga
     6721 ctcgaaaaac gcgctggcaa gttcgagatc ctctcgaccc acaactcgtt tcacggccgc
     6781 acgctggcga cgttgaccgc gaccggccag gaaaagatcc gcgcgggcta tgatccgctg
     6841 ccggtgggat ttcgtcaggt gccctataac gatctgaatg ccacggaagc cgcgatcgat
     6901 ggccagaaga ccgtcgcgat tctcgtcgag cccattcaag ccgagggcgg agtcgtcgtc
     6961 gccaacgaga tttatatgct cgggctgcgc gaaatctgcg atcaacgcgg ccttttgctg
     7021 atttttgacg aggtgcagac cggcatgggg cggaccggaa aacttttcgg ctacgaacac
     7081 ttcggcgtga agcccgacat catgactttg gccaaagcgt tgggcggcgg tttaccgctg
     7141 ggtgcgatgc tggcgcggga agaaatcgcc gccagcttcg tcgccggaag tcatgcgtcg
     7201 acgttcggcg ggaatccagt gtcctgcgcg gccggcttgg cagtcatgaa cgctctcatc
     7261 aaaggcggcg tgctgaagaa ttgcgtgcag atgggcaaat atttcgtcaa aggcttagag
     7321 gcgctgaaga agcgattttc atttatccga gagattcgcg gcaaggggct tctcctcggc
     7381 ctcgaattga cgatcgaagg gaacaaaatt gccgaagtct gcatgcaaga agggttgctg
     7441 atcaattgta cggcgtccaa ggttctgcgt ttcgcgccgc cgctcacgat caccaagcgt
     7501 gaaatcgacc gcggcttggc aattctagaa aatgttctgg cccgccagtg agtcggcctc
     7561 ctctcatgca atgagcaaac gtgacctagt caattttgcc ggcctgagcc ggcgcgatct
     7621 tttcgttatt ttaggcctc
//
LOCUS       DASULZ010000098         2673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_315501, whole genome shotgun sequence.
ACCESSION   DASULZ010000098 DASULZ010000000
VERSION     DASULZ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315501"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VFY96_02000"
     CDS             <1..210
                     /locus_tag="VFY96_02000"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX5605253.1"
                     /translation="ELAPQSGAPWMRSLMISHHGRMLPMGGAVPILNGVTIVGAIGVA
                     GASRPEQDVLCCRAAMAVWEPPGHH"
     gene            complement(483..1505)
                     /locus_tag="VFY96_02005"
     CDS             complement(483..1505)
                     /locus_tag="VFY96_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605254.1"
                     /translation="MITFWHKKLISLLAAFTLIGASHFCATAGEMVFPKAEKTRLEVG
                     TAGSGTTSLPLFVALDGGYFAKRGLMVSVNQVGATVAVQGIISGTLDIYQGGTAAIAA
                     NLAGADIMYVAAAVDRNSLILFGQKGITSFESLRGKSIATTFPGAFGEIAVRMTARKL
                     GMEVGKDFKLLYHRSPPEALTTFLAGNSDALIITPPQTELARQQGYPVIIDYYKEGLK
                     IVGPGSAVTREFAQRYPNTIKAFLMSYLDGLKRAIDDEEFATKTESKYSKITDPKILA
                     ANYQQGLRVWNKDMTVDVSAIRVVLEDSSDSRAKGADPNRFFDNTLVQAVNREYASKL
                     FPGEVK"
     gene            1826..2242
                     /locus_tag="VFY96_02010"
     CDS             1826..2242
                     /locus_tag="VFY96_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605255.1"
                     /translation="MRRLSLMLLLLSLTACATTPLTLNQTVAIPESRVLAPQFLSQTS
                     NAGSLVLKRDGGFMGSACTIRVFVDAVPVADLKPSERVELFVPLGEHSVVATATGAIC
                     GGGTSEAAVVIMPERQRILRIASGQSGDIFLQPSAF"
     gene            complement(2449..>2673)
                     /locus_tag="VFY96_02015"
     CDS             complement(2449..>2673)
                     /locus_tag="VFY96_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605256.1"
                     /translation="APAKASENADQLVTKITAALEKSHWFFADLLEKFPKADYKSIGS
                     AVARLYEEGKITQDQEGKFQLKATEISPAA"
BASE COUNT          566 a          765 c          707 g          635 t
ORIGIN      
        1 gagctcgcgc cgcagtcagg cgcaccctgg atgcgtagtc tcatgatttc acatcacggc
       61 cgcatgctgc cgatgggagg tgcggtgccg atcctaaacg gcgtcacgat tgtcggtgcc
      121 atcggagtcg ccggagccag ccggccggaa caggacgtgc tctgctgccg tgcggccatg
      181 gcggtctggg aacccccagg ccatcattag cacgagaaac tgcttttact tctccactct
      241 gtgtgtgaaa gctcgccgtt cgttcttcga gttggactta ggatgaacaa agggcgcaag
      301 gcgcatgtct ttcgatatgg cagcgctgtt cgctgcctat tcagaatggt cggtttaaaa
      361 accggttccc cgagtagcgc aagccgcgta catcgagggg cgagacaatc gaaccgcgac
      421 gcagcaccga ttacacctgt ggcctgtgac acgactcacg cctgactcct cacctctgtc
      481 cgtcacttca cttcccccgg aaataacttc gaagcatact ctcggttcac cgcctgaacg
      541 agcgtattat cgaagaatcg gttgggatcg gcgcctttag ctctggagtc ggaagagtct
      601 tccagcacga cacgaatggc ggagacatcg accgtcatgt ctttgttcca cacccgcagt
      661 ccctgttggt agttagccgc aagaatcttc ggatcggtga tcttgctata tttagactcg
      721 gtcttcgtgg cgaactcttc gtcatcgatc gctcgcttga gcccatcgag gtaactcatg
      781 agaaacgcct tgattgtgtt cgggtatctt tgcgcgaatt cgcgagtaac cgcactcccc
      841 ggcccgacga tcttcaatcc ttccttgtaa taatcgatga tgaccggata gccctgctgc
      901 cgggccaact cggtttgcgg gggcgtgatg atgagcgcgt cggagtttcc cgcaagaaaa
      961 gtcgtcagcg cctccggcgg actgcgatga tagagcagct tgaaatcttt cccgacctcc
     1021 atgccgagtt ttcgtgcggt catgcgcacg gcgatttcgc cgaacgctcc cgggaacgta
     1081 gtcgcaatgg atttgccgcg cagactttcg aaagatgtaa ttcccttttg gccgaataaa
     1141 atgagcgaat tgcgatcgac cgcggcggcg acgtacatga tatcggcgcc ggcgagattc
     1201 gccgcgattg cggccgtgcc gccctgataa atatcaagcg tgccggaaat aattccctgc
     1261 accgccaccg tggcgccgac ttgattgacc gaaaccatga ggccgcgttt ggcaaaatag
     1321 ccgccgtcga gagcgacaaa caacggcaaa ctggtggttc ccgatccggc cgtgccgacc
     1381 tcgagtcgag ttttctccgc tttgggaaat accatttccc cggccgtcgc gcagaaatga
     1441 cttgctccga tcagagtgaa tgcggcaagc aatgaaatta attttttatg ccaaaaagtt
     1501 atcattgttg ttactctccg tacccacgac caatctctga tgtcatccta agcgcgctca
     1561 gattacgttt cacatcctcg tgaagtgtac acgatacgtt gaggcgttac gaaatcgtcc
     1621 ggtattttcg tacgagcaaa tgactgccga acgcgcccct cacctaaatc cggggcaagc
     1681 ggcatctcat tcggcaacgt ctgtgcccgc gaagcgagtg cccccgaagc cgcctaaccg
     1741 ctgaattgct atcggattca atctctctgg gttggaaact cgctaagact cgtactatag
     1801 tcccgaactc ggccttggag gatgtatgcg ccgcttatcg ctgatgcttc tccttctgtc
     1861 acttaccgct tgcgccacga cgccgctgac gcttaatcaa accgtcgcca tacccgaatc
     1921 ccgcgtctta gctccacagt tcctgagtca aacttcaaat gcgggaagtc tcgtcctgaa
     1981 gcgcgacggc ggctttatgg gatcggcgtg tacaattcgc gtattcgtcg acgccgtccc
     2041 ggttgccgat ctcaaaccgt cggagagagt cgaattgttt gtcccgcttg gcgaacacag
     2101 cgtggttgcc accgcaaccg gcgctatttg cgggggaggc acttcagagg cggcagtggt
     2161 aatcatgccg gagcggcaaa gaattttgcg catcgccagc ggtcagagcg gcgatatttt
     2221 tcttcagccc agcgcgttct gaacgggttc tgctaaaaac tcccctgccc caacttgcca
     2281 aagaggggtt gcaccccgct tgattcagaa caacaggctt tcgcccacgt gagcaccgtt
     2341 gcgtgctggt ttgaacgaac tgcatcgtga gcttgattga acatttaacg ggctgcatgt
     2401 cttccagtat gccgcaatga ctcttcaagt actcgttgag atgttccgtt acgctgctgg
     2461 tgagatttct gtagccttca gctgaaactt tccctcttga tcctgagtaa tctttccttc
     2521 ttcgtacaat ctcgcaacgg cgctaccgat agacttgtaa tctgccttgg gaaacttctc
     2581 caagagatcc gcaaaaaacc agtgggattt ttcgagagcg gcggtaatct tagtcaccag
     2641 ttgatcggca ttctcggaag cttttgcagg agc
//
LOCUS       DASULZ010000099         2677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1025681, whole genome shotgun sequence.
ACCESSION   DASULZ010000099 DASULZ010000000
VERSION     DASULZ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025681"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VFY96_02020"
     CDS             <1..475
                     /locus_tag="VFY96_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605257.1"
                     /translation="QKEAEEELIARERRVDRFKIVPLSREDAARFSEDWRAVQNRFVD
                     DPKASVEDADRQVRDLMQRRGYPMGDFEQRAADLSVDHAEVVEHYRTAHQIAVRNRNQ
                     GGNATTEDLRKAIVHYRAIFDELLEVGRPRERAHEEKKGGLAQWKFGKDLFRRQI"
     gene            436..885
                     /locus_tag="VFY96_02025"
     CDS             436..885
                     /locus_tag="VFY96_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005164107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605258.1"
                     /translation="MEVRERPLSTADLAGKPETHDERERRDAEATATIDKRDAAVPIE
                     QPAAKGPRAAGSNNEELVPLFSESAVQNFRQRWTTLQTEFVDEPRRSVEQADELVAHV
                     MKDMAATFSDERKKLEQQWEQGDKVSTEDLRLVLRRYRSFFDRFLSI"
     gene            975..2555
                     /locus_tag="VFY96_02030"
     CDS             975..2555
                     /locus_tag="VFY96_02030"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEX5605259.1"
                     /translation="MKKIFYWIGGTIAVLVILVIVLSYAIDEPLRRKMEGDLNNRLKG
                     YSVRIGHLDFHPIGLSLDLERAVIYQTAQPDPPVAEIPSLSASVHWKALLFGHLVADF
                     EINEPKVHFDLRQFAQEAKDEVPMKDKGWQDAVQAIYPLKINRFAIYNGDLTYVDKGP
                     FKPLHITKVNFVTENIRNVQSAEGTFPSPVQADGVVFDRGTIKVNGNADFLAEPHVAL
                     NLAAQLDQIALDYFKPIAERYKLAVQQGVLSADGSFQYTREGTRLEIKQLRLDNFVAD
                     YIHTTAEAPTKKVVKETGKVAREHANEPTFELKVDQVQVRNGRLGYVNRAAKPPYHVF
                     FANTNLTIENLSNHLKEGIAHVRAEGKFMGSGPAKIDAAFRPENKGPDLNLAVSIDDT
                     DMRSMNDLFRAYGNFDVVAGKFSFYSELKVRQGKIDGYVKPLFRDMDVYDQRQDKEKS
                     LFRKLYEGLVGGISGLLQNKPREEVATKIPVSGDIESPQTRTWETVGNLVENAFFKAI
                     LPGFERQVAQGKGEGGRG"
BASE COUNT          662 a          684 c          759 g          572 t
ORIGIN      
        1 ccaaaaggaa gcggaggagg aactgatagc gcgcgagcgc cgtgtggatc gtttcaagat
       61 cgtgccgctt tcgcgtgaag atgcggcgcg gttcagtgag gattggcgcg ccgtgcaaaa
      121 tcgttttgtc gacgatccaa aagcatccgt cgaggacgct gaccggcagg tgcgcgactt
      181 aatgcaacgg cgcggatatc cgatgggcga tttcgagcag cgagcggcgg atctgtccgt
      241 agatcatgct gaagtggtcg agcactatcg gacggcgcac caaatcgcgg tccgaaatcg
      301 aaatcagggc ggcaacgcca ccaccgagga tctcagaaaa gctattgttc actaccgcgc
      361 gatcttcgat gagttgttag aagtgggcag accgcgcgag cgagcccacg aagaaaaaaa
      421 gggaggattg gcacaatgga agttcgggaa agacctcttt cgacggcaga tttagctgga
      481 aaacctgaaa cccacgatga gcgtgagcgc agggacgctg aagcaactgc gacgatcgat
      541 aaacgcgacg ccgctgttcc catcgaacag cccgcggcaa aagggcctcg agcggcgggc
      601 agcaacaatg aagaactcgt tccactcttt tctgaatcgg cggtgcagaa ttttcgccaa
      661 cgatggacca cgcttcaaac cgagtttgtc gatgagcccc ggcgctcggt cgaacaggcg
      721 gacgaactcg tggctcacgt gatgaaggat atggcggcga ccttttccga cgagagaaag
      781 aagctcgaac agcaatggga gcagggtgat aaggtttcca ccgaagactt acggctggtc
      841 ctgcgccgtt atcgctcatt tttcgatcgg ttcctttcga tttagttcga cggttcctcg
      901 caattcatcc tatggtcggc tctgccgtag cgtggccgac tcgcagtcca tttgtcacgg
      961 gtgtggggac agacgtgaag aaaatatttt actggatcgg tggcacaatc gctgttcttg
     1021 tgatcctcgt catcgtgctc tcgtacgcca tcgacgagcc gctccgtcga aagatggagg
     1081 gcgacctcaa caatcggctt aagggctact cagttcgcat cggccatctc gattttcatc
     1141 ccataggact ctccctcgat ctcgaacgcg cagtcattta ccagaccgcg cagccggatc
     1201 caccggtggc ggagatccct tccttgagcg ccagcgtgca ctggaaagca ctgcttttcg
     1261 gtcatttggt cgcagatttc gaaatcaacg agcccaaagt gcacttcgat ctaaggcaat
     1321 tcgcccaaga agcgaaagac gaggtgccca tgaaggataa gggatggcaa gacgcggtgc
     1381 aggcgatcta tcctctaaaa atcaatcgct tcgcgattta caacggcgat cttacctatg
     1441 tggataaggg tcctttcaaa ccgttgcata tcacaaaggt caactttgtc actgagaata
     1501 tccgcaatgt tcaatcggca gaaggcacgt ttccttcgcc cgtacaagcg gatggcgttg
     1561 tttttgaccg cggtacaatc aaagtgaacg gcaatgcgga ttttctcgca gaaccgcatg
     1621 ttgctctaaa tctggcggca cagctcgatc aaattgcgct ggactacttt aagccgatag
     1681 ccgagcgcta caaattggcg gtgcaacaag gagtgctatc ggccgacggg tcctttcagt
     1741 acacgcgaga gggaactcgg ctcgagatca agcaattacg tctcgataat tttgtcgccg
     1801 attacatcca tacgaccgcc gaagctccca ccaagaaagt tgtcaaggaa accggcaagg
     1861 tggcaaggga acacgctaat gagccgacct ttgagctcaa ggtggatcaa gtgcaagtcc
     1921 gtaacggccg tctggggtat gtgaatcggg cggcgaaacc tccgtatcac gtgttttttg
     1981 ccaacacgaa cttgaccatc gagaatctga gcaaccatct caaagagggc attgcgcacg
     2041 ttcgagcgga aggcaagttc atggggagcg gacccgcaaa aatagatgcg gcattccgtc
     2101 cggaaaacaa aggacccgat ttgaacctgg cggtcagcat tgatgatacg gatatgcgct
     2161 ccatgaatga tctgtttcgc gcctacggga acttcgatgt ggtcgcgggg aagttttctt
     2221 tttactcgga gttgaaagtc cgacagggca agatcgacgg ctacgtcaag ccgctgtttc
     2281 gtgatatgga cgtttacgat cagcgtcagg ataaagaaaa gagtcttttt cgcaagcttt
     2341 atgaggggtt agtcggcggt atttccggcc tcctgcagaa taaaccgcgt gaggaggtgg
     2401 cgaccaaaat tcctgtctct ggggacatcg agtcgccaca aaccaggacc tgggaaaccg
     2461 tgggcaatct agtagaaaac gcttttttca aagccattct ccccggcttt gagcgacagg
     2521 tcgcgcaagg caaaggggaa ggtgggcgcg gctaatcttt ggccattcac ctcttcacaa
     2581 ctcctgtaag cactggcgct gcggatttgc atttgcacgc ctacggttct gtcgaataaa
     2641 gtccgaacgc tgaagtattc atcggcggag gaacaga
//
LOCUS       DASULZ010000100         3495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_518839, whole genome shotgun sequence.
ACCESSION   DASULZ010000100 DASULZ010000000
VERSION     DASULZ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518839"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VFY96_02035"
     CDS             <1..537
                     /locus_tag="VFY96_02035"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX5605260.1"
                     /translation="THDQAEALAISDQIAVMHGGVLIEVGSPHQLYTRPKRRFTATFL
                     GLTNLIEGKVVEIDGDGKPGKLQTKKGVLSFIPSTDLKKNQAAVISIRPENIPLYKER
                     PQGLENVLEGKVTEAIFMGDAYQCKIAVGDDLLAVHTHPFNAVNLGDIVYLQLDPRSC
                     NGLPADDREGIEESMLGD"
     gene            574..2064
                     /locus_tag="VFY96_02040"
     CDS             574..2064
                     /locus_tag="VFY96_02040"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX5605261.1"
                     /translation="MTALEARKLAEDEMKAGKTPRYRDLRDWLEIVDSIGELKKVDGV
                     DWNLEMGTLAEIFARQSKTGVTPAVLFDNVPGYPKGYRTLFAQNASFKRMALNLGLDM
                     NLSSLDLVRAFRQKLVTHEAIKHKVVTKGPVLENVDSGDKVDLLKFPVPFMHELDGGR
                     FIGTACLVTTRDPDEGWVNFGAYRGMVHDKNSMGLYISPGKHGSIQRNKYFERGKPCP
                     VIVSVGQDPVLFMVSGNEIDYGVSEFDYAGGLKGEPIEVIEGKSTGLPFAANAEIVIE
                     GWMDPTDRKTEGPFGEWTGYYASSSRPEPVIRVENIYYRNDPILCCARPGRPPSDYSI
                     AKCMVKASLIWEQVEKAGVPDVRGVWCHEAGGGRLLNIISIKQRYPGHARQAMLAASQ
                     VHAGAYLGRYVIVVDEDIDPTNTFDVLWALASRSDPVESIEILRRCWSGPLDPRIHPS
                     KKGFNSRAVIDACRPFEWMKDFPPVAESSPELRDKIYNKWKKFIEA"
     gene            2223..3284
                     /locus_tag="VFY96_02045"
     CDS             2223..3284
                     /locus_tag="VFY96_02045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5605262.1"
                     /translation="MRYGLFYLPSSLPCTRADGAERFRTVVDQVSFGEEIGFDSVWLA
                     EHHFHSFAGLFSSPPVIGAAIAQRTTRMRIGTAVLLLPYHNPLRVAEDYATLDCLSNG
                     RVEFGVGHGFIKWEALTFGIALDELRDRFKENLEIVLKAWTAPALHHRGRFQKFNGVE
                     VYPRPVQEPHPAVWMAATTSVESFELSGRSGFHMMLIPFLNEVEELRAKMQAYFSARK
                     AAGHDPSSARVLGVYHAYVGESSVEARAAAAPGLAEYHSAASQAHGLTPGMADPASYR
                     SHEQHRAQMKQLTFDDLVEQSRVLVGSAAEVRDKIEYVRERLYLTDLAGNFALGGLTD
                     AQVRASMRRFMQQVAPKIR"
BASE COUNT          789 a          926 c         1023 g          757 t
ORIGIN      
        1 actcacgatc aagctgaagc gctggcgatt tcggaccaga tcgccgtgat gcacggcggc
       61 gtgctgatcg aagtcggttc gccgcatcaa ctttataccc ggccgaaacg cagatttaca
      121 gcgacgttcc tcggattgac caaccttatc gagggcaagg tggtcgagat cgacggcgac
      181 ggcaagccgg gaaagttgca aaccaaaaaa ggggttttga gctttattcc atcgaccgat
      241 ctaaaaaaga accaggctgc ggtgatctcg atccggccgg aaaatattcc gttgtacaag
      301 gagcggccgc aaggattgga aaacgtgctc gagggaaaag tcaccgaagc cattttcatg
      361 ggcgatgctt atcagtgcaa gattgccgtg ggcgatgact tgctggcggt tcatacgcat
      421 ccgtttaacg ccgtcaatct cggtgacatc gtttatctgc agttggatcc gagaagttgc
      481 aatggtcttc cggcggatga tagggaaggt attgaggaat ccatgctcgg ggactagaat
      541 cctcgaaaca gcatctgaaa ggagactgtc cagatgacag cactagaggc taggaagctg
      601 gccgaagatg aaatgaaagc cgggaagact cccaggtacc gagacttacg cgactggctc
      661 gagatcgtcg acagcattgg cgaattgaag aaggtcgatg gcgttgactg gaacctcgaa
      721 atgggcacgc tggcggagat cttcgcccgg caatcgaaga cgggtgtaac gccggcggtt
      781 ttgttcgata acgttcccgg ttacccgaaa ggctatcgaa ctcttttcgc gcaaaacgct
      841 tcattcaaac ggatggcgtt gaatcttggc ctcgacatga acctttccag tttggacctg
      901 gtgcgtgcgt tccgtcaaaa gctcgtcacc cacgaagcga tcaaacacaa agtcgtcacc
      961 aaaggtccgg ttctggaaaa cgtcgactcg ggtgacaaag tcgacctgct caagtttccc
     1021 gttcctttta tgcatgagct cgacggcggt cgctttatcg gtaccgcctg tctggtcacg
     1081 acgcgtgacc cggatgaagg atgggtaaac ttcggcgctt accgaggcat ggtgcacgac
     1141 aagaacagca tgggattgta catttcgccg ggcaagcacg gcagcattca gcgcaacaaa
     1201 tacttcgagc gcggcaaacc gtgtccggtg atcgtctccg tcggtcagga tccggtgctc
     1261 ttcatggtct cgggaaacga gatcgactac ggtgtttcag aattcgatta tgccggcgga
     1321 ttgaagggcg agccgatcga agtgatcgaa ggcaaatcca cggggcttcc tttcgccgcc
     1381 aacgcggaaa tcgtcatcga gggctggatg gatccgaccg accgaaaaac cgaaggtccg
     1441 ttcggcgaat ggaccgggta ctatgcgagc tccagccgtc cggagccggt gatccgggtc
     1501 gaaaacattt actatcgtaa cgatccgatc ctctgctgcg cgcgtcccgg gcgtccgccg
     1561 tcagattact ccatcgctaa atgcatggtg aaagcgtcgt taatttggga gcaggtcgaa
     1621 aaagccggcg ttccagacgt tagaggcgta tggtgtcacg aagccggcgg cggtcgcttg
     1681 ctaaatatca tttcaatcaa acagcgctat cccggtcacg cccgccaggc gatgctcgcg
     1741 gcgtcgcagg tgcatgcggg agcgtatctc ggccgctatg tcatcgttgt cgatgaagac
     1801 atcgatccga ccaatacttt cgacgtgctc tgggcgctcg ccagccgttc cgatccggtg
     1861 gaatccatcg agatcctccg ccgctgctgg agcgggccgc tcgatccccg tattcatccc
     1921 agcaagaagg gttttaactc tcgcgcggtc atcgatgcgt gccggccgtt cgaatggatg
     1981 aaagatttcc cgccggttgc ggagtcttca ccggaattgc gcgacaagat ttacaacaag
     2041 tggaagaagt ttatcgaagc gtagttaaag ttacgacttg ggtaagcggg gcgaccgtga
     2101 tgagcggtcg cccctttttt ttttgatcga taaagcaatt cgttgacaga gttgattcct
     2161 cttaattcac ctcttaacga tttcatcttg tcatccccaa ttcgctgacg tatggtccgg
     2221 ccatgcggta cgggctcttc tatttgccgt cgtcgcttcc atgtacgcgc gccgacggcg
     2281 ctgagcgctt tcgaacggtt gttgatcaag tttcgttcgg agaggagatc ggcttcgact
     2341 cggtctggct cgccgagcat cacttccatt ctttcgccgg attgttttct tcgccaccgg
     2401 tgatcggcgc ggcaattgcg cagcgaacga cgcgaatgcg tatcggcacg gcggtgctct
     2461 tgctgcccta tcacaatccg ctccgcgtgg cggaggatta cgccactctc gattgcttga
     2521 gcaatggaag agtggagttt ggcgtcggcc acggcttcat caagtgggaa gcgctgacgt
     2581 tcggaattgc gctcgacgag ctgcgtgatc gattcaagga aaatctcgaa atcgttctca
     2641 aggcgtggac agcacctgcg ctccatcacc ggggacgatt tcaaaaattt aacggcgtcg
     2701 aggtttatcc gcgcccggtg caagagccgc atcctgcggt ttggatggcg gcaacgacct
     2761 cggtggaatc cttcgagctc tcgggccgct ccggctttca tatgatgctc attccttttc
     2821 tgaacgaggt cgaagagctt cgcgcgaaaa tgcaggctta tttcagcgcg cggaaggccg
     2881 ctggacacga tccgtcgagc gctcgcgtcc tcggcgtgta tcatgcctac gtcggtgaaa
     2941 gctccgtaga ggcgcgtgcg gccgcagctc caggacttgc ggaataccat tcggctgcga
     3001 gccaagctca tggcctgacg ccgggaatgg ccgatccggc atcttatcga agtcacgaac
     3061 aacaccgtgc gcagatgaag caactcacat tcgacgactt ggtcgagcaa agccgcgtgc
     3121 tggtcgggag cgcggcagaa gtgcgcgaca agattgaata cgtgcgcgag cggctttact
     3181 tgacggattt ggcgggaaac ttcgcgctag gaggattgac cgacgcgcaa gtgcgcgcat
     3241 ccatgcgccg attcatgcaa caggtagcgc cgaagatccg ctgagccggt agcattcacc
     3301 atccgatgag gctagcattg acatgcaatt gactgaggaa ctataaccag ccagaccggt
     3361 tataaaaacc ttcagtacat gctacgcaaa aagccttaca ggcgaggcgg gcgaaacatg
     3421 gatgagcgga ggcgtactca gagcgtacgt cgtagcgaag caattgagcg caacgaagca
     3481 tatgaggctt tttca
//
LOCUS       DASULZ010000101         2579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_11530, whole genome shotgun sequence.
ACCESSION   DASULZ010000101 DASULZ010000000
VERSION     DASULZ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11530"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..220)
                     /locus_tag="VFY96_02050"
     CDS             complement(92..220)
                     /locus_tag="VFY96_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605263.1"
                     /translation="MTNRAEVYVEGEWKQERYISHFVLRSLSPANLAKFYREVYEL"
     gene            complement(230..403)
                     /locus_tag="VFY96_02055"
     CDS             complement(230..403)
                     /locus_tag="VFY96_02055"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5605264.1"
                     /translation="MGRQAGLEHFGLEVEDVDKVAARLKEKYPAIQLVKRPTNRPFAG
                     ISTHDPDGYVFDL"
     gene            863..1963
                     /locus_tag="VFY96_02060"
     CDS             863..1963
                     /locus_tag="VFY96_02060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX5605265.1"
                     /translation="MEQETRSLADLAAEIDDWLAAQKDFVNADQFAGILRTLLRLAKD
                     NAERGDLKILNRALQELRRAFSIFAPYRHVRKVSIFGSTRVKEIDPYYHLARSFAEEL
                     ARAGLMVITGAGPGIMQAGHEGAGREKSFGVNIRLPSVQDANPFIRNDPKLMNFHFFF
                     TRKLMFVKEADAVVIFPGGFGTHDELLESITLAQTGKTHLVPIILMDVPGGTYWRRWQ
                     EFLREDVVSRGYITEKEMTFFRIATSADAAVQEINNFYRNFHSYRFVKHDLVIRLNYP
                     PSAELIERLNRDFADILKDGKVQQTEPLPAEADDPGKLHLHRLRMRFNREDYARLRQM
                     IDVINAEELSDHAEPIVPERRQTKSDAELSSE"
     gene            2067..2276
                     /locus_tag="VFY96_02065"
     CDS             2067..2276
                     /locus_tag="VFY96_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HEX5605266.1"
                     /translation="MSEHVYKQIELTGTSKTSVEDAVQNAITKAAQTLHSIHWFQVVE
                     TRGYIENNAVDYWQVTIKLGFRLDD"
     gene            complement(2322..>2579)
                     /locus_tag="VFY96_02070"
     CDS             complement(2322..>2579)
                     /locus_tag="VFY96_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605267.1"
                     /translation="GVNRAGSKPWLIIQVPLDGEVIDCAYDYLHANSEPTLYPPADAV
                     ENLIKMSGYMDKKLQSISAKQVVDLTLLDELGTKQNERVHR"
BASE COUNT          575 a          692 c          732 g          580 t
ORIGIN      
        1 cgtaatccga gatggcccag ggtgagatta tcatggtcac gcggctgtcg gttaaatagt
       61 gatttcgatc gtcggcgggt ttctcgagct cctagagctc gtaaacttcg cgatagaact
      121 ttgccaaatt ggccgggctc aaagagcgga gcacaaaatg gctgatatag cgctcctgct
      181 tccattcgcc ttcgacgtac acctccgcgc ggttcgtcat cgcctgctgc tatagatcga
      241 agacgtagcc gtccgggtcg tgggtgctga tcccggcaaa cggccgattc gtcgggcgtt
      301 tcaccagttg aatcgcgggg tacttttctt tcaatcgggc agcgaccttg tcgacatcct
      361 cgacttccag accgaagtgc tccaggccgg cttgtcttcc caaactttcg cgggatgata
      421 ttcatgccgg cgtaaccgat cgccaccgcg ctctccggcc gaggcgattt cgatgttcgc
      481 ctgccgaaga gagcttcata gaaccggctc aacaaaacat aattgtcgct gagagtggca
      541 aggtgtttta gtttcgcgaa catggcaccg cgaccgataa caagtaaatc tgaaacgtgt
      601 cacgctgata accgtgcgac tatcttgggt ggtcgttgat ggatggagca ggatctcact
      661 gagtgtctcc tggccctgcc gaatggttcg agatgacaga cgattactgt gttgtcattg
      721 cgagcaaagc gagaaatctt tcttaaatat cactcgacgg ttggaccgct gctgcggcag
      781 cgcaaacgtt cactatcgag gggcggctcc atcgtctata ctgtcgtaga cgggaatttc
      841 tctccgagag aaggtggcgt aagtggaaca ggaaaccaga agcttggcgg atctcgcggc
      901 ggagatcgac gactggctgg ccgcgcagaa agattttgtc aatgccgatc aatttgccgg
      961 catcctcagg actttgctca ggctcgcgaa ggataacgcg gaacggggcg acctcaaaat
     1021 tcttaaccgc gcattgcagg aactgcgccg cgccttcagc atctttgcgc cctatcggca
     1081 tgtgcgcaag gtcagcatct tcggttcgac gcgggtcaaa gaaatcgacc cctattacca
     1141 tttggcgcgg agcttcgccg aggagcttgc tcgagcaggg ttgatggtga tcacgggcgc
     1201 cggaccgggg atcatgcaag ccgggcacga aggtgcgggc cgggagaaga gcttcggcgt
     1261 caatatccgt cttccctcgg tgcaggacgc caatcccttc attcgcaacg acccgaagct
     1321 catgaacttt cattttttct ttacgcgcaa gctcatgttc gtcaaagaag ccgacgcggt
     1381 ggtgattttt ccgggcggct tcggcactca cgacgagctt ctggaatcga tcaccttggc
     1441 acagaccggc aaaacccatc tcgtgccgat tatcctgatg gatgttcccg gcggaacgta
     1501 ctggcgccgg tggcaagagt ttctccggga agatgtcgtg agccggggtt atattaccga
     1561 aaaagaaatg acttttttcc gtatcgccac gagcgccgat gcagcggttc aagagatcaa
     1621 caacttttat cgtaacttcc attcgtaccg attcgtaaaa catgatctag tgattcgcct
     1681 gaattatccg ccgtcggccg aattgatcga gcgcctcaac cgcgacttcg ccgatattct
     1741 gaaggacggc aaagtgcagc aaacggagcc cctgcccgcc gaagcggatg atcccggcaa
     1801 gctccatttg catcgtctgc gcatgcgttt caatcgcgaa gattacgcgc gcttgcgtca
     1861 gatgatcgac gtcattaacg cggaggaact atccgatcat gccgagccga ttgtcccaga
     1921 aagacgacaa accaagagcg acgcggaact ctccagcgag taacgctaac cgttccctat
     1981 ttgattttag ccgtttaatc gcgacctgac gccggggtac tgttcttgca cacagctcta
     2041 gcaaattcac gcgaccggag ggcggcatga gcgagcatgt ctacaagcag atcgaattga
     2101 ctgggacgtc gaagacgagc gttgaagacg cggtgcaaaa tgcgattacg aaagccgccc
     2161 agacacttca cagtatccac tggttccaag tcgtcgaaac gcgcggctat atcgaaaaca
     2221 acgcggtcga ttactggcag gtgacgatca aactcggctt caggctggac gattaggcag
     2281 aaatcggtcc gggggtaggg cagatccgtg cgcggcgtgg gttatcgatg cacccgttcg
     2341 ttttgcttcg tgccgagctc atctaaaagc gtgagatcga cgacttgctt ggcgctgatc
     2401 gattgcagct tcttgtccat atagccggac attttgatta gattctctac agcgtcggcg
     2461 ggcggataga gggttggctc tgagttggcg tgcaggtaat cgtacgcgca gtcgatcacc
     2521 tcgccgtcga gcggcacctg aatgatgagc cagggtttac tgccggcgcg attgacgcc
//
LOCUS       DASULZ010000102         2623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_924557, whole genome shotgun sequence.
ACCESSION   DASULZ010000102 DASULZ010000000
VERSION     DASULZ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924557"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VFY96_02075"
     CDS             complement(<1..582)
                     /locus_tag="VFY96_02075"
                     /inference="COORDINATES: protein motif:HMM:NF045289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605268.1"
                     /translation="MDIALLQQPLFYVGGHHITVLGLIAFIGFFAAGLIIARFLQSQV
                     IRRFFSRFKIDTNFIAIVTTTLSLAVIVFFTVTAVNAAGIPLAWTAPLPAISLSLVQI
                     FLLIALLVGVFWFSSGTKRFLFNRVLAQSGLERSLQYAIAQVVSNIVLVVGIFFVLEN
                     AGIHLAALAVFAGAVGVGVGFGLQNIASNFISGL"
     gene            complement(691..942)
                     /locus_tag="VFY96_02080"
     CDS             complement(691..942)
                     /locus_tag="VFY96_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX5605269.1"
                     /translation="MADKPIEEKVKDIIVEQLGVNPEQVTPQASFIEDLGADSLDIVE
                     LVMAFEEEFGVEVPDEDAEKLQTVGDVIKYIEEKASKQQ"
     gene            1393..1800
                     /locus_tag="VFY96_02085"
     CDS             1393..1800
                     /locus_tag="VFY96_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605270.1"
                     /translation="MPRKGSNKRPDFDRVKDRRVPERAAQPPLKQFEGSIRFFPRESV
                     FENVVAQIKSGSVAYSLFVLARLFLEKPGRYEVRLTAKTEAPLYQLGENGGISVDREL
                     LERNAFRVAQRDFYQVNVVESDPIKGNFSNVAR"
     gene            1942..>2623
                     /locus_tag="VFY96_02090"
     CDS             1942..>2623
                     /locus_tag="VFY96_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605271.1"
                     /translation="MVERWKEEARKTTTYTTLREETPLTFSSAAEAEKHFRSRYLPAL
                     TRNVEDATIGGMPSRSLPDRILNRAIEEAWVRETRSPSNMMQELASRFRQIGLHIFRH
                     RRGMLFVSPIRARAFVHEHTGVSSSVNAILEAVSATAGISRKQLFETLTGNGASDDAE
                     PRRLALASDLRWLINEGYLIEFNDGSLDRPRMKSKQPRQPPEALGVSGITVTTTTWQL
                     QLTWPERKL"
BASE COUNT          648 a          712 c          716 g          547 t
ORIGIN      
        1 cagaccgctg atgaaattac tggcgatatt ttgaaggcca aatccgacgc cgacgccaac
       61 cgccccagca aaaaccgcca gtgcggcgag atggattccg gcgttctcca gaacaaagaa
      121 gataccaacc accagcacga tgttgctgac gacctgggca atggcgtact gcaacgatcg
      181 ttcgagccca ctttgcgcca gcacccggtt gaaaagaaaa cgcttcgtcc ccgaggaaaa
      241 ccaaaacacg cctaccaaca gcgcgatcag cagaaaaatt tgaaccaggc tcagactgat
      301 tgctggaagc ggcgcagtcc atgccagcgg aattcccgca gcattgaccg cggtgacagt
      361 gaaaaagaca atgactgcca ggctcagggt cgtcgtgacg atcgcgatga aatttgtatc
      421 gatcttgaac cggctgaaga aacgccgaat gacctggctc tgcaaaaacc gggcgattat
      481 cagtccggcg gcgaaaaacc caatgaaggc gattaacccg aggaccgtta tgtgatgacc
      541 gccgacgtaa aacagcggct gctgcaggag cgcaatgtcc acgtcctagt gtgaacgtgt
      601 catggggctt ggcaaccagc gcttatggag gcatgcagtc gttagaacgc ctgaagtttg
      661 caacgaccgc cgattgcgat ccgaatggcg ttactgctgt ttggacgcct tttcttcgat
      721 gtatttgatg acgtcgccga cagtttggag tttttccgca tcttcatccg gtacttcgac
      781 cccaaattcc tcttcaaaag ccatcaccag ttcgacaatg tccaatgaat ctgcccctaa
      841 atcctcaata aaagatgcct gcggcgtgac ctgctctggg ttcacgccga gttgctcgac
      901 aatgatgtcc ttgaccttct cttcgatcgg tttatccgcc ataaaatttg ttcggttggt
      961 gttggttgcg cgggggtaag tccacgcagc tgccggaaaa tccggccaca gcatcgcaag
     1021 taaatgaatt aaagaggatg tcaatcattt tcgggagcaa tggcaaaacg tgctgcgatc
     1081 cggatgcgga acgtgaattg aaaggcgcca gccgttcgcg tttgcttccg tttgctgttc
     1141 gagtagatga acgtgatgag cgctccgccc gtcgagcccg ccgatcttct tgatctgaag
     1201 ttattgcctt catgggtgaa agagcccggg gctacgaatc actacgatca ctacaccgga
     1261 gaagaggccc gtgaagaatc gcgtgggcgc catcgagatg gcaggcacaa agatcgaaaa
     1321 tcgcggggcc gcgaccagag acgagacacg cgacgaccgg tatccaggtc acagacgggc
     1381 gaccgtaaga gaatgcccag aaaaggaagc aacaagcgtc ccgattttga ccgggttaag
     1441 gatcgccgcg tgcccgagcg cgccgcccag ccgccactaa aacagttcga gggatcgatt
     1501 cgttttttcc cacgtgagag tgttttcgaa aatgttgtgg cgcagatcaa gtccggctcg
     1561 gttgcctatt cgctgtttgt tctggcccgc ttgttcctgg aaaaaccggg acgttatgaa
     1621 gttcgtctca ccgcaaagac cgaagcgcct ctttatcaac tcggcgagaa cggcggaatt
     1681 tccgtagacc gggaattgct ggagcgaaac gccttccggg tcgcacaacg cgatttttat
     1741 caggtcaatg tcgtagaaag cgatccgata aagggaaatt tctcgaatgt cgccagatga
     1801 cgtttgagcg gcacgctgct tggcccaacc aatcaccacg cttaccagcc gcaattacgt
     1861 agcttgtacg agcaacggtt tagccggcgc atgagctttg ccgattatca acggcaaatc
     1921 gatatcgtca gcgatcccgc agtggtcgag cgctggaaag aggaagccag aaagacgacg
     1981 acttacacca cattgcggga agaaacgcct cttacctttt cgtctgctgc cgaagcggaa
     2041 aaacatttcc gttcgcgcta tttaccggca cttactcgaa acgtggagga cgcgactatc
     2101 ggcggcatgc cgagccggag tctgccggac cggatcctga accgggcgat cgaagaggcg
     2161 tgggtgcgcg aaacccgctc tccttcaaac atgatgcaag agctggcgag ccgattcagg
     2221 caaattggtc tgcatatctt tcgtcatcgg cgtggcatgc tgtttgtttc gccaattcgc
     2281 gcccgtgcgt ttgttcatga acacaccggc gtctcttcgt ctgtgaacgc gatacttgaa
     2341 gcagtctctg cgactgcagg gatcagtcga aaacagcttt ttgaaactct gactggcaat
     2401 ggagcgagcg acgacgcgga accgcgcagg ctggcgctcg cgtccgacct gcgttggctg
     2461 atcaacgaag gttacttgat cgagttcaac gacggttcac tcgatcggcc acgaatgaaa
     2521 tcaaagcaac cgcgacagcc tccagaggct cttggtgtaa gtgggataac cgttaccacc
     2581 acaacctggc aactacagct gacctggcca gaacgtaagc tac
//
LOCUS       DASULZ010000103         2092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_417416, whole genome shotgun sequence.
ACCESSION   DASULZ010000103 DASULZ010000000
VERSION     DASULZ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417416"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2092)
                     /gene="uvrA"
                     /locus_tag="VFY96_02095"
     CDS             complement(<1..>2092)
                     /gene="uvrA"
                     /locus_tag="VFY96_02095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX5605272.1"
                     /translation="LLFVRAGRLHCPDCGSEIVAQSTEQIVDQLLSLPHGTRLIVLAP
                     LALSSEIKADKLLAEVARQGFSRAMVDGKTVELGGEMSADWSKARQLDVVIDRLIVRE
                     GIRTRLVEAVELAGRQGAQVVKVLILEDGDYESAVERRFSKAPKCLNCGAAAPEVTMS
                     LLSFNNPEGACPTCNGLGEIPEHAKNVEQTTYACPECNGTRLKEESRLMRIGGHDISQ
                     ISSWPIADAMEFFCALEFSGDRVLVGKKVVGEITRRMRVLTHLGLNHLSLDRSSLTLS
                     GGEIQRVRLGTEIGSGMTGVLYVLDEPSRGLHQRDNAQLLDLLKGLREAGNSVIVVEH
                     DPETMRAADYLIDMGPGAGERGGEVVAQGSFAEILADEQSLTGRYLAGELTISTRAAR
                     RNGSGKYLLIRNARMHNLKNITVQVPIGALTCVTGVSGAGKSTLVMDVLYEELRRRLH
                     KSKSGKPPAADLVGWENFDRVIGVDQQAIGRTPRSNPATYTGVYDYLRGFFSELPEAR
                     VRGYQPERFSFNQKGGRCEACAGDGVTRVEMYLIPDLSVICPVCKGRRYNRETLEVRY
                     KGLSIADVLALTVDEALELLSNIPPIRERLQTLRDVGLGYVRLGQPASTLAGGEAQRV
                     KLARELARKSSGQALYVLDEPTAGLHFEDTKKLLDLLKRLTDSGNTVVVVEHNLDMIK
                     NADYVVDLGPESGVG"
BASE COUNT          460 a          641 c          523 g          468 t
ORIGIN      
        1 gccgacgccg ctttccggcc ccaagtcaac gacgtaatca gcgtttttga tcatgtctag
       61 attgtgctcg actaccacta cggtattgcc gctatccgtg agacgcttaa gcaagtccaa
      121 aagttttttt gtatcctcga aatggagccc agccgtgggc tcatccagaa cataaagagc
      181 ttggccggaa ctttttcgcg ccagctcgcg cgcgagtttt accctttgcg cttcgcctcc
      241 cgcgagtgtg gatgcgggct gacccagcct gacatagccc aatcccacgt cccgcaaagt
      301 ttgcagccgc tcgcgaatcg gtggaatatt gctgagtaat tcgagcgcct catcgacggt
      361 cagcgccaaa acatcagcga tgctcaaccc tttatatctg acctccaagg tctcacggtt
      421 atagcgccgg cctttacaaa ccgggcagat gacggaaaga tccggaatca aatacatctc
      481 cacccgtgtc actccgtcac cggcacaggc ttcacagcgg ccaccttttt gattgaatga
      541 aaagcgctct ggctggtagc cacggacgcg cgcttcaggc agctcggaaa aaaaaccccg
      601 aagataatcg taaacaccgg tatacgtggc cggattggat cgcggtgtgc gtccgatcgc
      661 ttgctggtct acgccgatga cacggtcgaa attttcccag ccgacgagat ctgctgccgg
      721 gggcttaccg ctttttgatt tatgcaaacg tcttctcaac tcctcataca aaacatccat
      781 gaccaatgtg cttttgcccg cgccggaaac accggtgacg caggtgagtg cgccaatggg
      841 aacctgtaca gtgatatttt ttagattatg catacgcgcg ttacgaatca gcaaatattt
      901 cccgctgccg ttccgtcgcg cagcgcgagt agaaatagtt aattcgcccg cgagataccg
      961 accggtaaga gactgctcat cggcaaggat ctccgcaaaa gagccttgcg ctaccacttc
     1021 accaccgcgc tcacctgcgc cgggacccat atcaataaga tagtccgcag ccctcatggt
     1081 ctcgggatca tgttcgacga caatcaccga attacccgcc tcgcgaagtc ctttcagaag
     1141 atcgagcaat tgggcgttgt ctctttggtg taacccgcgg ctcggctcgt ccagaacgta
     1201 gagcacacct gtcatacccg agccgatctc cgttcctagg cggaccctct ggatctcgcc
     1261 accggaaagg gtcagcgaag accgatccaa gctcaaatga ttcaaaccca aatgcgttag
     1321 aacacgcatt cgccgggtga tttcaccgac gactttctta cccaccagta ctcgatcgcc
     1381 ggaaaattcg agtgcgcaga agaattccat cgcgtccgcg atgggccagc tgctgatttg
     1441 cgaaatgtca tggccgccga tgcgcatcag gcggctttcc tcttttagcc gggttccatt
     1501 gcactccggg catgcgtagg tcgtttgttc cacgttttta gcgtgctcag gtatttcccc
     1561 gagaccatta caggtcgggc aagctccttc cggattgttg aacgacaaca agctcatggt
     1621 gacttccggt gcggccgcgc cgcagttcag gcatttgggc gctttgctga accgcctctc
     1681 aaccgcggac tcatagtcac cgtcttcgag gattagcacc ttgacgacct gagcgccctg
     1741 tcgacccgcg agttcgaccg cttccacgag ccgagttcga attccttcgc gcacaattaa
     1801 ccggtctatg accacgtcca actggcgcgc tttgctccaa tcagcggaca tttcgccgcc
     1861 aagctcgacc gttttgccgt cgaccatcgc ccttgaaaac ccttgccttg ccacttccgc
     1921 gagtagcttg tcggctttta tttccgacga aagcgccagc ggcgccagca cgatcaggcg
     1981 cgtgccatgg ggcaacgaca gcagttgatc aacaatttgt tcggtgctct gcgcgacgat
     2041 ttcactgcca cagtcgggac aatgcaaccg acccgctcga acgaacagca gg
//
LOCUS       DASULZ010000104         3861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1026216, whole genome shotgun sequence.
ACCESSION   DASULZ010000104 DASULZ010000000
VERSION     DASULZ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026216"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..738
                     /locus_tag="VFY96_02100"
     CDS             235..738
                     /locus_tag="VFY96_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605273.1"
                     /translation="MKKLIVLTDTEPRQSWANLRGLFIFLLLIDGCAQSMKHDETAAA
                     RRAIEFAQAAFVDRKFDQAYELLSTGGKRHLSLEKFKETVTRMHSRGFPTKVIAKEFQ
                     PMPGENAIWIYLAGQNSEDQFGYRLTMESDGHGDYKILTFDSGVVGRMFSPLSEKQPF
                     KTTFSTH"
     gene            775..1419
                     /locus_tag="VFY96_02105"
     CDS             775..1419
                     /locus_tag="VFY96_02105"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX5605274.1"
                     /translation="MIGTANVAQRRFGKAQKIRHSLWLLLSVTAVSLFFFGNGRSGSL
                     AIAIGQEKPVSIERATRPLIRVPTHLTLEEARLIIEAAKSVVKAENGRAAIAVVDFNG
                     NLVSLDSMDGTSGFWIRFAIGKAMGAATLQQSTAESADQLQTNPQRFHSALSLMQGQI
                     ILIPGGLPLIIDNVIVGAVGSAGHRGDGDVRAAKAGIAAWEKYRQSKPTEGAQK"
     gene            1444..1881
                     /locus_tag="VFY96_02110"
     CDS             1444..1881
                     /locus_tag="VFY96_02110"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5605275.1"
                     /translation="MPAEFIDILKMEELDIGHSKKVLFSSDHFHTWVHGDYPGTKGPM
                     HKHTADQQFYCVRGQCTFHFPDAPSRALNPGMLVTIPAGQLYQLDNTGSEYMILLGAR
                     AEPAGNPRFSAAGNEVGHGDLAKRNLDKITAPEQKKAKARLES"
     gene            1969..2559
                     /locus_tag="VFY96_02115"
     CDS             1969..2559
                     /locus_tag="VFY96_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605276.1"
                     /translation="MNATQRLPVFHQFIQDLRAAWAELPNAEARMKKGQQLLEALVKD
                     PTLREASKKWPSTEGRKNLLFYEDPDYGFAINGVVRVPGRKGSIHDHAHSWTAYGLLE
                     GTESLERYRRVDDGSKEGYARLELEGVTPGVPGKVDLVPPFDAHAEQGGPTRSVAVIL
                     RSERVSGKVLQGSYDLEANTVRRIDGPTNIPYEITG"
     gene            complement(2671..>3861)
                     /locus_tag="VFY96_02120"
     CDS             complement(2671..>3861)
                     /locus_tag="VFY96_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5605277.1"
                     /translation="AVTNGEKNLGFSTLAVHGGEPRPKLGNSLATPIIQTATYTFSNT
                     RELHDHFQRRIEREDYGRYGNPTQRIAEQKLAVLEGAEDCLLFSSGMAAVTTAVYAVL
                     SHNSHVVVTDDSYRRTRQFLTQTLHRYGIEVSTVPAGDYDAIEEAIRPTTRLLVSESP
                     TNPYNRIVDMERFAEIGRRHRVKTLIDATFATPYNQRPLEFGIDIVLHSATKYLAGHN
                     DLLAGAILGSVELVDGIRSLQHVTGAIIDPLAAYLLVRGLKTFALRLARQNANAQVLA
                     DFLAAHPKITAVHYAGLRTHPQHDVARRQMRGFGGVVSFEIAGDLEATSRVVDACRIP
                     QIAPSLGGVESLIEQPALMSFYELSTEERLTVGIKDNLIRYSVGIEDADDLIADLAAA
                     LEQA"
BASE COUNT          955 a         1072 c         1033 g          801 t
ORIGIN      
        1 ccaaaagcat cattttcatc cgcctatcga catccgccag acctcagccg gtctgcttcg
       61 gggtaaacta ggaatcagta gagtcttagt gtaaaccatg tgaggcagga agcagattac
      121 gccgactgat acttttagat gacaccacga tgtcggtact cacgttcttc atacaaacaa
      181 attacagtgg tataagacca gcatttttct aagcaagctt tgtccgaaaa agttgtgaaa
      241 aaactgatag tcctaacaga caccgagccc cgacagtcat gggcgaacct gcgcggactc
      301 tttatcttcc ttttattgat cgacggctgc gctcaatcga tgaaacatga tgagaccgcc
      361 gcagcgcgac gcgccatcga attcgcccag gccgcattcg tcgaccggaa gttcgatcaa
      421 gcatatgaac tgctctccac cggtggcaag cgtcatctgt cgctggaaaa attcaaagag
      481 acggtcaccc gtatgcacag tcgcgggttc cctacgaaag tcatcgcaaa agagtttcaa
      541 ccgatgcccg gtgagaacgc catctggatc tatctggcgg gacaaaactc cgaagatcag
      601 tttggctatc gactgaccat ggaatcggac ggtcacggag attataaaat cttgacgttc
      661 gacagcggtg tggtgggaag aatgttttcc cccttgagcg aaaaacaacc attcaagact
      721 acttttagca cgcattgacg attatggctt catagccggg caagcgaagg cgtaatgatc
      781 ggtactgcga acgtagcaca acggcggttc ggtaaagctc aaaagattcg acattcacta
      841 tggcttctgc ttagcgtcac ggcggtgtcg ctcttctttt ttggcaacgg ccgatccggc
      901 tcgctcgcca tcgcaatcgg gcaagagaag ccggtatcca tcgagcgcgc cacccgtccg
      961 ctgattcggg taccgaccca tttgaccctc gaggaagcgc ggctcatcat cgaagcagcc
     1021 aaatcagtcg tgaaggcgga aaatggtcgc gcggcgattg ccgtcgtgga ttttaacggt
     1081 aatcttgttt cgttggattc catggacggc acttcgggtt tttggataag attcgccatt
     1141 ggcaaagcca tgggagccgc cacactgcag cagagcaccg ccgaatcggc agatcagcta
     1201 caaacgaatc cgcagcgttt ccatagcgcg ctcagcctga tgcaagggca aatcattctc
     1261 ataccgggcg gattgccgct catcatcgat aacgtcatcg tcggcgccgt cggaagcgcc
     1321 ggccatcgcg gcgatggcga cgtgcgcgcg gccaaagcgg gcatcgcggc atgggaaaaa
     1381 taccggcaga gcaaacccac cgagggcgcg caaaaataga cgttcacatg atgggaggta
     1441 aatatgccag cagaattcat cgatattttg aagatggaag aactcgacat cggccacagc
     1501 aaaaaagtac ttttcagctc ggatcatttt cacacttggg tgcatggcga ttatcccggc
     1561 accaaaggac ccatgcacaa gcatacggcg gatcagcaat tttattgtgt ccgaggtcaa
     1621 tgcacgtttc atttccccga cgctccctct cgggcgctta atccgggcat gctggtaacc
     1681 attcccgcgg gccagcttta tcagctcgac aatacaggaa gcgagtacat gattctgctc
     1741 ggcgcgcgtg ccgaacctgc cggtaatccg cggttcagtg cggcgggaaa cgaagttggg
     1801 cacggcgatt tggccaaaag gaatctcgac aagattacag caccggaaca aaagaaggcg
     1861 aaggcgcgtc tcgaatcgtg attgccgcag tccggctgag gcttgactga aagccgataa
     1921 attgtcggcc aaggaattga tcagctactg attctgaagg agcgacatat gaatgcaacc
     1981 caaagactcc ccgttttcca ccaattcatt caggatctcc gcgccgcctg ggcagaatta
     2041 ccgaatgctg aggcgcgcat gaaaaaaggc caacaactgc tcgaggcatt ggttaaagat
     2101 ccgacgttgc gtgaggcttc aaaaaaatgg ccgtcaaccg aagggcgcaa gaatctgctt
     2161 ttttacgagg atcccgatta cggttttgcc atcaatggag tcgtgcgcgt gcctggacgc
     2221 aaaggcagta ttcacgatca cgctcattca tggacagcct acggattgct cgagggaacc
     2281 gagagcctcg agcgttatcg aagggtcgac gacggatcga aggaaggcta cgcacgactt
     2341 gaactggaag gcgttacgcc aggggtccct ggcaaagtcg acctggtgcc cccattcgat
     2401 gcccacgccg agcaaggcgg cccgacgaga tccgttgcgg tgatcttacg ttccgagcgg
     2461 gtctccggga aagtgctgca gggcagttac gacctagaag ccaataccgt ccgccggatt
     2521 gacggcccaa ccaatatccc atacgaaatt accggctaaa ttcacctgcg agacgcggag
     2581 tacacggacc gaatcaaaac caatcctccg tgacctccgt gtctctacgg tctaattgtt
     2641 tcgttcgaaa ctcctgtcaa ccgattggcg ctaggcttgc tcgagcgcgg cggcgagatc
     2701 ggcgatgaga tcgtcggcgt cttcaatacc gacggagtag cggatcaaat tgtctttgat
     2761 tcctaccgtc aggcgctctt ccgtactgag ctcgtaaaag ctcatcagcg ccggttgctc
     2821 aatcaagctc tccactccac ccaacgacgg cgcaatctgc ggaattcgac acgcgtcgac
     2881 gacgcgcgat gtggcttcga gatcaccggc gatttcaaac gaaaccacgc cgccgaagcc
     2941 acgcatttgc cggcgtgcaa cgtcgtgctg cgggtgagtt cgcagacccg catagtgcac
     3001 cgccgtaatc ttcggatgtg cggccaaaaa atccgcaagc acctgggcgt tggcgttctg
     3061 ccgggctaag cgtagagcaa aggtctttaa accccgcacc aacaaatatg cagcgagggg
     3121 atcaataatc gcacccgtca cgtgctgcag cgaccgaatt ccgtccacca actccaccga
     3181 acctaaaatc gctccagcca aaagatcatt gtgaccggcc agatatttcg tcgcggaatg
     3241 cagaactatg tcgatgccga attctagcgg acgctggttg taaggtgtag caaaggtcgc
     3301 gtcgatgaga gttttgacac ggtgacgacg gccgatttca gcgaagcgct ccatatcgac
     3361 gatgcgattg taaggattcg tgggcgactc actcaccaat aatcgggtcg tcggccggat
     3421 cgcttcctcg atcgcatcgt aatctcccgc cggcacggtc gaaacttcaa tgccgtagcg
     3481 gtgcagcgtc tgggtcaaaa actgccgcgt gcggcggtac gagtcatccg tcacgacaac
     3541 atgagaatta tgcgacagca cggcatagac ggcagtcgta accgctgcca tcccactcga
     3601 aaacagaagg caatcctccg ccccttccaa aacggcaagt ttctgctcgg cgatacgctg
     3661 ggttggattt ccataacggc cgtagtcctc acgctcgatt cgccgctgga aatggtcgtg
     3721 caactcccgc gtatttgaaa aagtgtacgt cgccgtttga ataattggcg tcgccagaga
     3781 attgcccagc ttagggcgtg gctcaccgcc gtgcaccgcc aacgtgctga aacctagatt
     3841 tttttcaccg ttcgtgacgg c
//
LOCUS       DASULZ010000105         7758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1128268, whole genome shotgun sequence.
ACCESSION   DASULZ010000105 DASULZ010000000
VERSION     DASULZ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7758
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128268"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /gene="uvrC"
                     /locus_tag="VFY96_02125"
     CDS             complement(<1..860)
                     /gene="uvrC"
                     /locus_tag="VFY96_02125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX5605278.1"
                     /translation="MKTVAEKLANLPSRPGVYLMRDKAGKVIYVGKAKDLRSRVRAYF
                     NSADERSQVQFLVQRIDDFETLVTTSDKEALILENNLIKQYKPRYNIRLKDDKSYLSI
                     KVTTAHAWPRIFATRKIVKDGSRYFGPFSSAVAARETIDIIEKHFLLRNCTDHNFRNR
                     SRPCLQYQIKRCMAPCVLPVPGEVYREHVRQAMLFIDGKRQELVAELRQKMQQKSEAL
                     EYETAARIRDQIQAVEKTLEKQRMVAHWGADQDIFGLYREGGFIEVQVLLVRQGKLTD
                     NLSYSLEDLE"
     gene            complement(1111..3156)
                     /gene="uvrB"
                     /locus_tag="VFY96_02130"
     CDS             complement(1111..3156)
                     /gene="uvrB"
                     /locus_tag="VFY96_02130"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX5605279.1"
                     /translation="MEKLRDRDQGQFRLAANFAPQGDQPQAIEKLVAGLVQERRNQVL
                     LGVTGSGKTFTMANVIARANKPTLVIAPNKTLAAQLYNEFRELFPDNAVRYFVSYYDY
                     YQPEAYVPSTDTYIEKDASINDEIDKLRHSATKALLERRDTLIVASVSCIYGLGSPEA
                     YFEMMLYLEEGMTIQRDQMLRKLVDIQYQRSDYDFHRGTFRVRGDIVEIFPAYEDRSG
                     LRIELFDDRIESIAEIDPIRGKALRRIDKATIYPASHYVTGAARMKQATRGIREELRA
                     RLKELREQHKLLEAQRLEQRTLYDLELLEEMGFCPGIENYSRHLTGRQPGEPPPTLLS
                     YFPADFLLFIDESHVTVPQIGGMYRGDRSRKETLVEYGFRLPSALDNRPLNFSEFEAM
                     LNRTIYVSATPAEYEFGVSEGAVVEQLIRPTGLMDPEIIVRPARSQVDDLLDEIRKRV
                     ERNERVLVTTLTKRMAEDLTEYYQDLNVKVRYLHSDIETIERVEIIRQLRMGQFDVLV
                     GINLLREGLDLPEVSLVAILDADKEGFLRSDRSLIQTIGRAARNVYGTVILYADSMTD
                     SMRRAIDETERRRRLQAAFNKKHGITPQSIVKALGSPLIKIYEADYFEIPVAAEKPAK
                     YGPAELSRLLRKLQKEMKQAAERLEFETAAELRDRIWELQKQELNLRDSALTSSADQ"
     gene            complement(3160..4161)
                     /locus_tag="VFY96_02135"
     CDS             complement(3160..4161)
                     /locus_tag="VFY96_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605280.1"
                     /translation="MKPIQNLIVLPLALALVLLGHYALAQPRPDKLRIGYAARAVAHS
                     VPYVAKEAGFFSEEGIDAEIIRTAGSIAPMALVANEVDLAIMSAYLMIPVAAQNRDVV
                     MLGGFSRYASMVFVARPEVKGAQDLKGKIVGIQRPGDAYEKSARFALRHLGLDADRDV
                     KMLALGTNDVMWSALQTGRVSATILSPPGTLFARKAGMNFMVDLTDLKLEYQGSTIAT
                     RRSLLEKNPQLATRALRAIIRGVHLFKTRKEETMRILGKFLGTTDREALEESWDYAAK
                     MPAKPYAVESAVQAVLDHLSETQPRFAQYRPSEFINSRLLTELDKSGYIDRLYGQTK"
     gene            complement(4500..4769)
                     /locus_tag="VFY96_02140"
     CDS             complement(4500..4769)
                     /locus_tag="VFY96_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605281.1"
                     /translation="MADLRNARNGDAKIMLGFIVRRCAADIGHTPTPAEFATWANGQQ
                     QNGKRLCLFGKEISPSIAKVMFNQPGRLVTVSAEGLARIWKSNGR"
     gene            complement(4894..5829)
                     /locus_tag="VFY96_02145"
     CDS             complement(4894..5829)
                     /locus_tag="VFY96_02145"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5605282.1"
                     /translation="MSDFYTAWLERSKNNQKIVDEAPRVARGKDLKWVHTPQDYKAAL
                     MIAPETGFPTGGSCLMRAEIPVGWHTGKHFHGEEAIYVESGEGFMVLDEKRYDFFPGT
                     VLHIPYRSPHQLFNTGKTAVGYLSGLAWHLEADVYMGKMEQLEECGENDPKVLAAFPK
                     EESQYWPEDGRRIAMHQNQQEKSDESKHGATYFLMGRSGKQNGFKATGVAISSIFVDL
                     AHTKSHSHAHPEAYLYALEGDGYSEIGGKRYDWNQGDAVHVPPGMMHHQHFNPYDRDM
                     KELRFEFGVRYWLVDQWKGYTTVDKHLKATTHMVD"
     gene            5967..7424
                     /locus_tag="VFY96_02150"
     CDS             5967..7424
                     /locus_tag="VFY96_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HEX5605283.1"
                     /translation="MSFTLVAVIALLFVVLVIRAGSFKSRQVTAEPTQTLAISRAAAV
                     AHLSQAIQLKTVSLKPPAANAGEFVALHALIARAFPHINRQLNKETVGEHSLLFTWQG
                     KDHRLKPMLLMAHIDVVPVDPTTESSWHYAPFSGEIADGYIWGRGAMDDKGSVFAILE
                     AVESLLTTNFRPERTMYLAFGHDEEIGGINGAAKIAALLRARHVELESILDEGLNVFT
                     GIISGLDAPVALIGIAEKGYLSLQLSVESAGGHSSMPPEHTAIGALIGALQKLQDAPF
                     PARLNRPTREMFEFLGPEMPFDKKLVLANLWLFSPLVEKTLAQSPRTNALLRTTLAPT
                     MLNGGITDNVLPSKASAVVNLRIVPGESTASASQYVRQAIADPTVSILPLSIHWEPSA
                     VSNVDAASFAILRKTIRQTIPTAVVGPALLVASTDSRHYRSLTKNIFRFLPVTVGPHD
                     ISRYHGIDERISVEDYERCIRFYAQLIINANAPMR"
     gene            complement(7439..>7758)
                     /locus_tag="VFY96_02155"
     CDS             complement(7439..>7758)
                     /locus_tag="VFY96_02155"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5605284.1"
                     /translation="FSFAVSRDARIAFGFLVAVGALSTLFETLLNTSIQLRVEEAFRG
                     RVSGFYGLTGGGLREFGGMQAGFIAEWTSAPVAITAGAVALALIGYIFLNFRITNWHV
                     SRT"
BASE COUNT         1661 a         2230 c         2061 g         1806 t
ORIGIN      
        1 aattccaggt cttccagcga ataagaaaga ttgtcggtga gtttaccctg gcgcaccaaa
       61 agcacctgaa cttcgatgaa cccgccctcg cgatagagac cgaagatgtc ctggtcggcg
      121 ccccaatgcg ccaccatgcg ttgcttctcg agggtctttt ccacggcctg gatttgatcg
      181 cggatgcgcg cagcagtttc atactccagc gcctccgact tctgctgcat tttttgccgg
      241 agttcagcga cgagttcctg gcgtttgcca tcgatgaaca gcatcgcctg gcgcacgtgc
      301 tcccggtaaa cctctcccgg aaccgggaga acgcaggggg ccatgcagcg cttgatctgg
      361 tactgcaagc aaggccggct gcggttgcgg aaattgtggt cggtgcaatt gcgcagcaaa
      421 aaatgcttct cgatgatgtc gatggtttcg cgtgccgcca cggccgatga gaacggtccg
      481 aagtaacggc tgccgtcctt gacgattttt cgcgtggcga aaatacgcgg ccaggcatgc
      541 gccgtggtca ctttaatgct gagataactt ttgtcgtcct tcagccgaat gttatagcgc
      601 ggcttgtatt gcttgatgag gttattttcg aggatcagcg cctctttgtc cgaggtggtg
      661 accagcgtct cgaaatcgtc gatgcgttgc actaggaatt gcacctgcga tcgctcgtcc
      721 gcgctgttga aataagcgcg tacgcgcgag cgcagatcct ttgccttgcc gacgtaaatc
      781 accttgccgg ctttgtcccg catcaagtag acgccaggac gggaaggcaa gttcgcaagc
      841 ttttcagcaa ccgtcttcat cttttaaaaa aaccctatgc gccaaggatc cacgtccatg
      901 tggtgctggc ttggcatagc ttcgcctact atgccatttc gctccttgtc taatattcct
      961 gcttagattc tatcgcaaat gatgcggcta aatgtactct attgctggga attggtttgt
     1021 ctgacgggga gctaggcggg gcagctagag gtgaatgtaa ctcggtccga ccttggctcg
     1081 cgctaagcac gaacaacggc aggcctaaaa tcactggtcg gcgctggagg tcaacgccga
     1141 gtcccggagg ttcaactctt gtttctgtaa ctcccaaatt cggtcccgta attccgccgc
     1201 cgtttcgaat tccaaccgct cggctgcttg cttcatctct ttttgcagtt tgcgcagcag
     1261 gcgcgacaat tcggccggtc cgtacttcgc tggtttctcc gcagctacgg gaatctcaaa
     1321 ataatcggcc tcgtaaatct tgatgagcgg cgatcccagc gcctttacaa tcgactgcgg
     1381 agtgatgcca tgcttcttgt taaaggccgc ttgcaaacgc cgccggcgct cggtttcgtc
     1441 gatcgcccgc cgcatcgaat cggtcattga gtcggcatac aaaatcaccg tgccgtacac
     1501 gttgcgcgcg gcacgtccaa tggtttggat caacgagcgg tctgagcgca aaaatccttc
     1561 tttatcggca tcgagaatcg cgaccagtga gacctccggt agatcgagac cctcgcggag
     1621 caaattgatc ccgaccaaca cgtcaaattg tcccatgcgc agctgccgaa tgatttcgac
     1681 tcgttcaatc gtctcgatgt cggaatgcag gtaacgtact ttgacattta agtcttgata
     1741 atactccgtc agatcttccg ccatgcgctt ggtcaaagtc gtcaccagga cccgttcatt
     1801 gcgctctacc cttttgcgga tctcgtcgag caggtcgtcc acctgactgc gtgcgggccg
     1861 gacaatgatc tccggatcca ttaagccggt ggggcgaatc agctgctcga cgacggcgcc
     1921 ttcgctgaca ccaaattcgt attctgccgg cgttgccgaa acataaatcg ttcgattcag
     1981 catcgcttcg aattcgctga agttgagcgg ccggttgtcg agggcggatg ggagacgaaa
     2041 accatactcg accaaggttt ctttgcgcga gcgatcaccg cgatacatgc caccgatctg
     2101 cggcaccgta acatggcttt catcgatgaa gagcaaaaag tccgccggga aatagctgag
     2161 caaggtcggc ggtggctcgc ccggctgacg acccgttaga tggcgtgaat aattttcaat
     2221 ccccgggcag aaccccatct cttccaaaag ctcgagatcg tacaaggttc gctgctccag
     2281 ccgttgcgct tccaggagct tgtgttgctc gcgcaactct ttgagacgcg ctctcagctc
     2341 ttcccggata ccgcgggttg cctgcttcat gcgcgcggcg ccggtgacgt aatggctcgc
     2401 cggatagatg gtcgctttgt cgatgcgccg cagcgctttg ccgcggatcg gatcgatctc
     2461 ggcaatcgat tcaatgcgat catcgaaaag ctcgatcctc agtccggagc gatcctcgta
     2521 ggcgggaaaa atctccacga tatcgccccg cacccgaaac gtaccgcgat gaaaatcgta
     2581 atcgctgcgc tgatactgga tatccaccag cttgcgcagc atctggtcgc gctggatggt
     2641 cattccttct tcgagataga gcatcatctc gaagtacgcc tccggcgagc cgaggccata
     2701 gatgcaggaa acgctagcaa cgattagcgt atcgcgccgt tcgagaagcg ctttggttgc
     2761 ggaatggcgc aacttgtcga tctcgtcgtt gatcgacgca tctttctcga tgtaggtatc
     2821 ggtggacggc acataagctt ccggctggta gtaatcataa tagctgacga aatagcgcac
     2881 cgcattgtcc ggaaagagct cgcgaaactc attgtacaac tgcgccgcca aagtcttgtt
     2941 gggtgcaatc acaagcgttg gcttgtttgc acgggcaatc acgtttgcca tggtaaaagt
     3001 tttacccgag ccggtgactc ccaatagcac ctgattgcgc cgctcttgca cgagtccggc
     3061 gacgagcttt tcgatcgctt gcggctgatc gccctgcgga gcaaaattcg cagcgagccg
     3121 aaactgccct tgatctcgat cgcgaagctt ttccatccgt tatttggttt ggccgtaaag
     3181 ccgatcgata taaccgctct tatcgagctc cgtcaggagc cggctattga taaattcgct
     3241 cgggcggtat tgagcgaagc gcggctgagt ttcacttaaa tgatcgagca ccgcctggac
     3301 cgcgctctcc acagcataag gtttggccgg catcttcgcc gcatagtccc aagactcttc
     3361 caatgcttcc cggtcagtgg tgccgagaaa ttttccgaga atgcgcatgg tctcttcctt
     3421 gcgcgttttg aacaagtgca ccccgcggat aatggcgcgg agcgcgcgcg tcgcgagctg
     3481 cggatttttc tcaagcaatg accggcgggt cgcgattgtc gagccttgat attccagctt
     3541 gaggtccgtg agatcgacca tgaaattcat tccggctttg cgtgcgaaca gagttcccgg
     3601 gggagagaga atcgtcgccg agactcggcc ggtctggagc gcgctccaca tgacatcgtt
     3661 ggttcccagc gcgagcattt tgacgtcccg atccgcatcc aatcccaaat gacgcaacgc
     3721 gaaacgcgcg cttttctcgt aagcgtcgcc ggggcgctgg attccgacga tcttgccttt
     3781 taagtcctgc gcgcccttca cttcgggtcg cgccacaaag accatcgaag cgtagcgcga
     3841 aaaaccgccc agcatgacaa catcgcgatt ttgcgccgcc accggaatca tcaaataggc
     3901 cgacatgatc gccagatcca cctcgttggc caccaacgcc atcggcgcga tcgaacccgc
     3961 ggtgcgaatg atttcggcgt cgattccttc ttcgctaaaa aaaccggctt ccttcgccac
     4021 gtacggaacc gaatgggcca cggcccgtgc agcgtacccg atgcgcagct tatccggcct
     4081 gggttgagcg agcgcgtaat ggccaagtag cactagcgcc agagccagag gtaagacgat
     4141 caaattttga atcggtttca ctgcttattc aattgaccgt tgtcgagcgt gcgagcgctg
     4201 aaagcataac aagcgaaggc gatggcggca acgtttaccg ttctagggtt ggttttctcc
     4261 gtttattgtg aaagctcaaa ctcagagccg tacagctcca cgctctcctg ttgctattgg
     4321 agaactgccg caacgaactt ttgtatgagc tttaagcctt cgaacgacaa accggtcgga
     4381 gcttgattct gctatcttgc ctgcggcagt tctgctcgct tacggctgat cagcaaaatt
     4441 cccgaccgac gaagcgctgc agttttgggt ttggaactat tgagggcggc agaatgaagc
     4501 taacggccgt tgctcttcca gatccttgct agaccttctg cgctgacggt gacgagccgg
     4561 cccggctgat tgaacatcac tttagcgatc gatggcgaaa tttccttgcc gaagagacag
     4621 agacgtttgc cgttttgctg ttggccattt gcccaggtcg caaattcggc aggtgtgggg
     4681 gtgtgaccga tatcagcggc acaccggcgc acgataaagc cgagcatgat tttggcgtcg
     4741 ccatttcttg cgttgcgcaa atccgccatc actaattcta tagaagatcc agggtgacgt
     4801 tgcaagaaat aaatggcgcc ggctgaatcg ctgcaagctc gaagaaaaag ctaaccaatc
     4861 gtcagactgg ctcgcttttg tttcgttaaa aaactaatcg accatgtgcg tggtcgcctt
     4921 cagatgcttg tccaccgtgg tgtaaccctt ccactggtcg accagccagt agcggacgcc
     4981 gaactcaaag cgcagctcct tcatatcgcg atcataggga ttgaaatgct gatgatgcat
     5041 catgccagga ggaacgtgaa ccgcgtcgcc ctgattccaa tcatagcgct tgccgccgat
     5101 ctccgaatag ccgtcgcctt caagagcgta gagataagcc tcggggtgtg cgtgactgtg
     5161 gcttttggtg tgcgccaagt cgacaaagat cgagctgatc gctacgccag tggccttgaa
     5221 gccattttgt tttccgctcc gccccatcag gaaataggtt gcgccgtgct tcgactcgtc
     5281 ggatttttcc tgctggtttt ggtgcatcgc aatacgccgg ccgtcttcgg gccaatattg
     5341 cgattcctct ttggggaacg cggccagaac cttgggatcg ttttcaccgc attcctcgag
     5401 ctgctccatt ttacccatat aaacatcggc ctcgaggtgc caggcgagtc cggagagata
     5461 acctaccgct gttttgcccg tgttgaacaa ctgatgtggc gatcgatagg gaatgtgcag
     5521 gaccgtcccc ggaaaaaaat cgtagcgctt ctcatcgaga accataaatc cttcgccgct
     5581 ctcgacgtaa atcgcttctt cgccatggaa atgcttgccg gtatgccagc ccaccggaat
     5641 ctccgcacgc atgagacagc tacctccggt ggggaatcca gtttccggcg cgatcatcaa
     5701 tgccgctttg tagtcttgcg gtgtgtgcac ccatttgaga tccttgccac gcgccactcg
     5761 cggcgcttca tcaactattt tctggttgtt ttttgaccgt tccaaccagg ccgtataaaa
     5821 atcgctcatt gtccctccag ttttttgccc tttgtatatc ccaaaaaaat tatccgaccc
     5881 tcctgcgctt gtcaaattaa ggtcgctctg attcactcga tgacctgact tgctgactgt
     5941 tccctcaatt cacaatgatc aaaaaattga gcttcacgct tgttgccgtg atcgcgcttt
     6001 tgttcgttgt gctcgtgatc cgcgctggga gtttcaaatc ccgccaagtt accgccgaac
     6061 cgactcaaac gcttgcgatc agccgtgcag cagccgtagc gcacctctcg caggcgattc
     6121 aactcaaaac ggtttcgcta aaaccgccgg cagctaatgc cggtgaattc gtcgctttgc
     6181 acgctctcat cgcgcgcgcg tttccccaca tcaaccgcca attgaacaaa gaaaccgtcg
     6241 gcgagcacag tttgcttttc acctggcagg gtaaagacca ccggctgaag ccgatgctgc
     6301 tcatggcgca catcgatgtg gtgccggtgg atccaacgac ggaatcttcc tggcattacg
     6361 cgcctttttc tggagagatc gccgacggct acatctgggg acgcggcgca atggatgaca
     6421 agggtagcgt tttcgcaatt ctagaagcgg tcgagtctct gctcacgacc aatttccgtc
     6481 ccgagcgcac gatgtatctg gctttcggtc atgacgaaga gatcggtggc ataaacggcg
     6541 ccgccaaaat tgccgcgctt ttacgcgcgc gccacgtgga gctcgaaagc attctcgacg
     6601 agggactaaa tgtttttacc gggattattt ctggtctcga tgcgccggtg gcgctgatcg
     6661 gtattgccga gaaaggctat ctcagcttgc agctaagcgt cgaaagcgcc ggcgggcata
     6721 gctccatgcc tcccgagcat accgcaatcg gcgctctgat cggagcgctg caaaagctgc
     6781 aagatgcgcc gtttcctgcg cgcctcaata gaccaacgcg cgaaatgttt gaatttctcg
     6841 gtccggaaat gcccttcgat aaaaagcttg tgctcgcgaa tctgtggctg ttcagtcctt
     6901 tggtggagaa aaccctcgcg caatcaccgc gcaccaacgc gctcctccgg acaacgctcg
     6961 ccccgaccat gcttaacggc ggcatcacag acaacgtgct gccgtcgaaa gccagcgctg
     7021 ttgtcaatct tcgtattgtg ccgggcgaat cgacggcgag cgcgagtcaa tacgtccgcc
     7081 aagcgatcgc cgatcccacg gtaagtattc tgccactttc catccattgg gaaccgtcgg
     7141 ctgtatcgaa tgtcgatgct gcaagcttcg caatactccg taaaaccatt cgtcaaacga
     7201 tcccgacggc cgtcgtcggg cccgccctgc tcgtcgcctc caccgattcg cgccattatc
     7261 gcagcttgac gaagaatatc tttcgctttc tgccggtgac agtcggaccg cacgatatca
     7321 gcagatatca cggcatcgat gagcggatct cggtggaaga ctacgagcgc tgcatccgat
     7381 tttacgcgca gctgatcatc aacgctaacg cgccgatgcg ttgagattcg cgccgccttc
     7441 aggtccgact gacgtgccaa ttcgttatgc ggaagttaag aaagatgtag ccgatcaatg
     7501 cgagcgccac cgcacccgca gtgattgcca ccggcgcgct tgtccattcg gcgataaacc
     7561 cggcctgcat accgccgaat tcgcgcaagc ccccgccggt cagaccataa aatccagaaa
     7621 cgcggccgcg aaaagcttct tccacgcgca gttgaatcga agtgttgagc aatgtctcga
     7681 ataacgtgct caaggcgccg accgcgacca aaaaaccaaa ggcgatccgg gcatctcgac
     7741 tgaccgcgaa cgagaaca
//
LOCUS       DASULZ010000106         3033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_417848, whole genome shotgun sequence.
ACCESSION   DASULZ010000106 DASULZ010000000
VERSION     DASULZ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417848"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VFY96_02160"
     CDS             <1..426
                     /locus_tag="VFY96_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605285.1"
                     /translation="PVTFGAQKKGFYKVLDVGAMVDMPGGGLTALVKTIQERPSETKR
                     VIRSLQQAKDEIRKSKPKSLDLIVKLLKMDREAASETYDQFLTTLSPTGIPTRVGMDI
                     LVKSVQAQGRHVGRKVGFEEIADDRLATEVAKEMGYKIP"
     gene            655..1704
                     /locus_tag="VFY96_02165"
     CDS             655..1704
                     /locus_tag="VFY96_02165"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605286.1"
                     /translation="MIQRRRIKFDKRAFARALLTIVVVIICASGSWSQEKPTKIRISN
                     AGFTITALPLLAAKDWGVFSANGLDVELIVMSPTLAAAAVAQGDIDYVAGVGPASVAA
                     TLSGLPSRAIWFSSDRISYWLQSAPSYKTLQDLKGKKIALSGGVGGTNHVALVIALEK
                     SNVNPKDYVMVGIPGQQAQVFYSLDSSFVEAALLSPPHMFIAARKGFNRLLDVGAMVE
                     MPGGGLTTLIKTIQSRPSEVKRVIHALQLAKDEVRKSKAKTVELIMRLLKMDKQAASE
                     TYDAFQTTLNPTGIPASTISCDRCRRKDASPIVKSPSAKSPTIVWRRKWPGNWVTNCR
                     DREGTFPSPCGREVM"
     gene            1795..>3033
                     /locus_tag="VFY96_02170"
     CDS             1795..>3033
                     /locus_tag="VFY96_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5605287.1"
                     /translation="MSPINCDVLVVGAGLAGFTAATRAAERGAKVLLIEKSAGELGDG
                     NILMASGSLRAGGKSPRTHPRELYDFVMAEGIAHPDLVRAWSETCGRAVDWLINSGVK
                     VDENAPGRVWLDQSGEVSLAPVYKKDVGTRALTKLKERFLSFAGRYENSIQGKTLIVE
                     KGRVTGMIGTRAGEKIEFRAHATILATGGFSANEDLIKKHIGPHADECKLRGSKQDTG
                     DGLRMALAAGAKAVNLKYFYGHLISRKALVDDRFWPYPRLDCFVDEGILVNRDGMRFV
                     DEGRGDVAVANELARSQDVTGAVLIFDDDSWQAAKDDSFSNLVKTPAPNPWLMDNGGE
                     IFRSETIEGLARVLGMDAERLRSTVNDYNRAVDTAANPSLGVSRSGKPKLLRPCYHGL
                     RVVPGITFTMGGILISGRGVA"
BASE COUNT          721 a          819 c          880 g          613 t
ORIGIN      
        1 ccggtaacgt tcggcgctca gaaaaaaggc ttttacaaag ttctggatgt gggcgcgatg
       61 gtcgacatgc cgggcggtgg cttgaccgcg ctggtaaaaa cgatccagga acggccgagc
      121 gaaaccaaac gcgtgattcg ctccctgcaa caggccaagg atgaaatcag aaagtcaaaa
      181 cctaaatcac tcgatctgat cgtgaagctg ctcaaaatgg acagggaagc agcgagcgaa
      241 acctacgatc aatttctcac caccctgagc ccgacgggaa ttccgacccg cgtagggatg
      301 gacatactgg tgaagtccgt tcaggcgcag ggccgacacg tgggacgcaa agttggtttc
      361 gaagaaatcg ccgatgatcg gctcgccacc gaagtcgcca aagagatggg ttacaaaatt
      421 ccatgacgcg ggcttcagcc tctgggatcc gtacatgaac gcggtctatt ccttctctga
      481 gactgcgcgg cggtcaaaaa aatacgcgcg tcggcagtgt cctacgaact cgacgaggcg
      541 tctcgacgtt cctacgtgtt gggaagacaa gattcttcgc cctgcagggc ccagaataac
      601 aaactccatg tcatcctgaa cgaagtgaag tatctttaaa atttaggacc agcaatgatt
      661 caacgccgta ggatcaaatt tgataaacgg gcattcgcgc gggcgctact gactatcgta
      721 gtagtcatta tatgtgccag cggcagttgg agccaagaaa aaccaaccaa aatccggatt
      781 tccaatgccg ggtttaccat caccgcattg ccgctgctcg cggcgaagga ctggggagta
      841 ttcagcgcca atggtttgga tgtcgagttg atcgtgatga gcccaacgct tgctgcggcg
      901 gcggttgccc aaggcgacat tgattacgtc gccggcgtgg ggccggcgtc cgtggcagca
      961 acattgagcg gcctgccctc gcgggcgata tggttctcct ccgacagaat ttcttactgg
     1021 ttgcagagcg caccttccta caaaacgcta caggacttga agggcaaaaa gatcgcgctc
     1081 tccggcggtg tgggcggaac caatcatgtc gctttggtga ttgcgctgga gaaatcaaac
     1141 gtcaatccaa aggattacgt aatggtcggc atccccggtc aacaggcaca agtattctat
     1201 tcgcttgact cgagcttcgt cgaggcggcg cttttatcgc cgccgcatat gttcatcgcc
     1261 gcacgaaaag gatttaacag actcctggat gtcggcgcga tggtcgagat gccaggcggc
     1321 gggttgacga cgttgatcaa gaccattcag tcgcggccaa gcgaagtaaa gcgcgtcatt
     1381 cacgcgctgc agctggccaa agacgaggtg cgcaaatcga aagcgaagac cgtcgagctg
     1441 atcatgcgac tcttgaaaat ggataagcaa gccgcctcgg aaacgtacga tgccttccag
     1501 acaacgctca atcccacggg gattccggca tcgacaatct cgtgcgatcg ctgcaggcgc
     1561 aaggacgctt caccgatcgt aaagtcgcct tcagcgaagt cgccgacgat cgtctggcga
     1621 cggaagtggc cagggaattg ggttacaaat tgccgtgatc gagaaggaac ctttccttct
     1681 ccctgtggga gagaggtgat gtgaaagctt cggaccctca ccctcgccct ctcccgcaag
     1741 cgggcgaggg aaaaacaaag atgccactcg taggtttcgt ttgcgaggtc aagcttgagt
     1801 cctattaact gcgatgtgct cgtcgtcggc gctgggcttg ccggcttcac cgccgcgacg
     1861 cgcgcggcgg aaagaggcgc caaagtttta ttgatcgaga agagcgcggg agaactgggc
     1921 gacgggaata tcctgatggc ctcaggcagc ctgcgcgccg gcggcaaaag cccgcgcacc
     1981 cacccacgcg agctttacga ttttgttatg gccgaaggca tcgcccatcc tgatcttgtt
     2041 cgggcgtggt cggaaacctg cggccgcgcc gtcgactggt taattaatag cggtgtcaaa
     2101 gtcgacgaaa atgcgccagg cagagtctgg ttggatcagt cgggtgaagt ctctctcgcg
     2161 ccggtttaca aaaaagatgt cggcacacgc gccctcacca aactcaagga gcggtttctt
     2221 agtttcgccg gccgctatga aaatagcatt caaggaaaaa cattgatcgt cgagaagggc
     2281 cgcgtaaccg gcatgatcgg aacgcgggcc ggcgaaaaaa ttgaatttcg cgcgcatgcg
     2341 acaattctcg cgaccggcgg cttcagcgcc aacgaggact tgatcaaaaa gcacatcggc
     2401 cctcatgcgg acgagtgtaa gctgcgcggt tcgaaacagg ataccggcga tggccttcgg
     2461 atggcccttg cagccggcgc caaagcagtc aacctgaaat acttctacgg ccatctcatt
     2521 tcgcgcaaag cgctcgttga cgaccgcttc tggccctatc cgcggctcga ttgtttcgtt
     2581 gatgaaggca ttctagtcaa tcgcgacggc atgcgcttcg tcgacgaagg tcgcggcgat
     2641 gttgcagtgg ccaatgaatt ggctcgatcg caagacgtta ccggcgcggt gctcatcttt
     2701 gatgacgata gttggcaagc agctaaagac gacagttttt ccaatctggt aaaaacgccg
     2761 gcgcccaatc cttggctaat ggacaatggc ggtgaaatct ttcgcagtga aaccatcgaa
     2821 ggcctggcgc gggttcttgg tatggacgcc gaacgactgc gttcaactgt caacgactac
     2881 aatcgcgccg tcgatacagc tgcaaaccca tcgctcggcg tctcacgaag cggcaagccc
     2941 aagctgttgc gcccttgcta ccatggcttg agagtcgtgc ccgggattac gtttacgatg
     3001 ggcggaatcc tgataagcgg ccggggtgtg gcc
//
LOCUS       DASULZ010000107         2925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1026507, whole genome shotgun sequence.
ACCESSION   DASULZ010000107 DASULZ010000000
VERSION     DASULZ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026507"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..553
                     /locus_tag="VFY96_02175"
     CDS             122..553
                     /locus_tag="VFY96_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605288.1"
                     /translation="MAYDVAKLLTMSQSELDALFTDSAAGNIPDGEADGTAIVAPGTT
                     YSATIAKFVSHFAWQGKVFDAKKGVLRNRILPFGLNAILAKVYKGPSWLDGKECIVLD
                     YSDTSIIAHWIRDEIRQIDPGLYLGKVYWDKKRLIDFALKF"
     gene            721..2241
                     /locus_tag="VFY96_02180"
     CDS             721..2241
                     /locus_tag="VFY96_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006101226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5605289.1"
                     /translation="MDERSASERVFSRIGLITVVFLVLAVLGGMFLLGRVGADVPVDY
                     NDDIDHFKYGSVGSEHEFGVPYWIWRALPELFQDKLPPPAQGWQSVGFVFEPGKDLPV
                     GMSKRHYLGFDVVWLNCAFCHAGTVRETPQSGPKVYAAMPANTFDFRAFMRFLFATGE
                     DRRFTPQDILRQIDTIRKREGLGRLPLIDRLVLRFYGIYYMRERILTLRDRLSFIKDE
                     PEWGPGRVDTFNPLKAYFNFPIEKLPQRERIGTTDFPSIWNQGQREALKMNLHWDGNN
                     ISLEERNRSAAMGAGITPPTGDRPRMKRVADWLRKLDAPPYPFKIDKTLAADGAPIYK
                     KYCAECHGADGKDFRGEYSGKVVPIDNIGTDRHRLDSYTYDVAVNQNMIFAEYGDERF
                     SHFRKTFGYANSPLDGIWLRAPYLHNGSVPTLRDLLQPSANRPKTFYRGYDVYDQKNV
                     GFVTDIAEEKGKKFFLYDTDEKKEPGNSNKGHEGKRYGTELSAAEKDALLEYLKTF"
     gene            2279..>2925
                     /locus_tag="VFY96_02185"
     CDS             2279..>2925
                     /locus_tag="VFY96_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605290.1"
                     /translation="MADGQQKGYRWKLALLVLVILAVVFGAVGWYKFFREEAQPAWVT
                     ATPDMRFMYGSIGAEHDAGLPYWIFFVLPRMFPEKLPGPGGYASLGVAWEQGQELPIG
                     FTKKVIGFPRVANNCASCHTASYRKSPNENPVFVPTGPNHTLNLQAFFRFAVDCAKDP
                     RFNADEIMSQIALVYDLPFLDKLIYRYVLIPITKKRLLEREKQFQWVYHKEFPLWG"
BASE COUNT          674 a          775 c          805 g          671 t
ORIGIN      
        1 aattttcttg acgaacaaag cccagctatg tgagattaag ccgcgccaag tcccgctcca
       61 tgcaagcatt actgcttcac cgcgaaattc aaactcagta aagcccacgg aggaacgctc
      121 gatggcttac gacgtcgcca aactgttaac tatgtcgcag tcggagctcg atgctctgtt
      181 taccgatagt gccgcgggca acattccgga cggggaagcg gacggcacgg cgatcgttgc
      241 gcccggcaca acttacagcg caacgatcgc gaaattcgtc agccactttg cctggcaagg
      301 aaaagtattc gatgcaaaaa aaggcgtgct gcggaatcgg attttgccct ttgggctgaa
      361 tgcaatactc gccaaagtgt ataaagggcc gagctggctc gatggcaaag aatgcattgt
      421 tctggattac tccgatacat caatcatcgc ccactggatt cgcgacgaga tccgacaaat
      481 agaccccggt ctatacctgg gaaaagttta ttgggacaaa aagcggctca ttgatttcgc
      541 gttgaagttt taatctgcag accgatcgcc ttcgcgcgtt tctatttcga gccgattcaa
      601 agtgctttgc cagcggttca tctaacctct ttcaggtctt gcatgatcac cggtgagagt
      661 cgcgctcttg ggaatcgagg gaatacgcat caacttggag accaaagggt ttgatttctt
      721 atggacgagc gcagcgcgag cgagcgagtt tttagtcgaa tcggattgat caccgtcgtt
      781 tttctcgtac tcgctgttct aggcggcatg tttttgttag gtcgcgtcgg cgcggatgtt
      841 ccggtcgact ataacgacga catcgatcat tttaagtacg gctccgtcgg cagtgaacac
      901 gaatttggcg tgccgtattg gatttggcgg gcattgccgg aattgttcca agacaagtta
      961 ccgccgcctg cccagggttg gcagtcggtc ggtttcgttt tcgaacccgg caaagatttg
     1021 ccggtcggca tgtcaaagcg gcactatctc ggcttcgacg tcgtctggct caactgcgcc
     1081 ttttgtcatg ccggcacggt acgcgagacg ccgcagagcg ggccgaaggt ctatgcggcg
     1141 atgccggcca acaccttcga ttttcgcgcc tttatgagat ttctcttcgc caccggcgaa
     1201 gatcgcagat tcactccaca ggacattttg cgccagatcg atacgattcg aaaacgcgaa
     1261 ggcctcgggc gattgccgct gattgaccga ttagtgctgc gcttttatgg aatctactac
     1321 atgcgcgagc gcattctcac gctgcgcgat cgtttgagtt tcatcaaaga tgagcctgaa
     1381 tggggaccgg gacgcgtgga tactttcaat ccgctgaaag cctatttcaa ttttcccata
     1441 gagaaattac cgcaacgcga gcgcatcggc acgacagatt ttccgtcgat ctggaatcag
     1501 gggcagcgcg aagcgttgaa gatgaacctg cattgggacg gcaacaacat ctcgttggag
     1561 gaacgcaatc gcagtgccgc catgggcgcc gggatcacgc cgccgaccgg agaccggccg
     1621 cgcatgaaaa gggtcgccga ttggctacgc aagctcgacg cgccgccgta cccgtttaaa
     1681 atcgacaaaa cgctcgctgc tgacggagcg cccatttaca agaaatattg tgccgagtgt
     1741 cacggcgccg acggcaagga ctttcgcggc gagtattcag gcaaagtggt gccgatcgac
     1801 aatatcggca cggaccgcca tcggctcgat tcttacactt atgatgtcgc ggttaatcaa
     1861 aacatgattt tcgccgagta cggcgatgag cgcttcagcc acttccgcaa gacctttggc
     1921 tacgcaaata gcccgctgga cggcatctgg ctccgagctc cctatctcca taatggttct
     1981 gtgccgacac tgcgcgattt gctccagccg agcgcgaacc ggccgaagac gttctaccga
     2041 ggctatgacg tctatgacca aaaaaatgtt ggcttcgtga ccgacattgc cgaagaaaag
     2101 ggcaagaagt ttttcctgta cgacacggac gaaaagaagg agccggggaa ttcgaacaag
     2161 ggccacgaag gcaaacgtta cggcacggaa ctctcagcgg ctgagaagga cgctttgctc
     2221 gaatatctta agacctttta acgccaaacc gggttaacga gtcacgattg gagaaatgat
     2281 ggcggacggg cagcagaagg gataccgctg gaaactcgca ttgcttgtgc tggtgatttt
     2341 ggcggtcgtt ttcggcgccg tcggctggta caagtttttc cgcgaggaag cgcaaccggc
     2401 ttgggttacc gccacgcccg acatgcgttt catgtatggc tcgatcggag cggaacacga
     2461 tgccgggctg ccctattgga tttttttcgt actgccgcgg atgtttcccg aaaagctgcc
     2521 gggtcccgga ggctacgctt cgctcggcgt agcgtgggaa caagggcagg aactgccgat
     2581 cggcttcacc aaaaaagtga ttggctttcc acgcgtagcg aataattgcg cttcctgcca
     2641 tacggcgagc tatcgtaaaa gtcccaacga gaatccggtt tttgttccta ccggaccgaa
     2701 ccatacgctc aatcttcagg cttttttccg tttcgccgtg gattgcgcca aagatccgcg
     2761 cttcaatgcc gatgagatca tgagccagat cgcgctcgtt tacgatttgc cgtttctcga
     2821 caagctgatt tatcgctacg ttctgattcc gatcacgaag aagcgtctgc tcgaacggga
     2881 aaagcaattc cagtgggttt accacaagga attcccactc tgggg
//
LOCUS       DASULZ010000108         8508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_925331, whole genome shotgun sequence.
ACCESSION   DASULZ010000108 DASULZ010000000
VERSION     DASULZ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8508
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925331"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VFY96_02190"
     CDS             complement(<1..477)
                     /locus_tag="VFY96_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5605291.1"
                     /translation="MAKRTKIPDFQAMAELRYQIRRFLRFSENAARKAGIEPQQHQLL
                     LAIKGLPNELKPTVGALAERMQLQHHSTVELIDRLVERGFVCRLRAADDRRQVLVKLT
                     RNGEEFLNSLSFHHLEELRSTGPKFVKILQRLIEIDDLADTENPSQPKLRSSRHKPG"
     gene            755..1069
                     /gene="rplU"
                     /locus_tag="VFY96_02195"
     CDS             755..1069
                     /gene="rplU"
                     /locus_tag="VFY96_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX5605292.1"
                     /translation="MKYAVIRTGGKQYRVSEGDIVKVEKLAGEVGAKVVLDNVLFIGG
                     NDEVKVGAPLVANAKVTGEIVGQGRAKKIIVFKKKRRKSYSRQHGHRQYQTALKITAI
                     EA"
     gene            1084..1341
                     /gene="rpmA"
                     /locus_tag="VFY96_02200"
     CDS             1084..1341
                     /gene="rpmA"
                     /locus_tag="VFY96_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX5605293.1"
                     /translation="MAHKKAGGSSRNGRDSQGQRRGVKVFGGESVRAGNILVRQLGTR
                     IHPGKNVGMGRDYTLFALIDGVVRYEHMDKHRKRVAVLPAQ"
     gene            1465..2499
                     /gene="obgE"
                     /locus_tag="VFY96_02205"
     CDS             1465..2499
                     /gene="obgE"
                     /locus_tag="VFY96_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX5605294.1"
                     /translation="MKFIDEVKIRVIGGDGGQGCVSFRREKFVPRGGPSGGNGGNGGD
                     VIAVADPQLTTLLDLRYQKLYKAGRGQHGMGSDCHGHRGDDRIIKVPVGTIVRDGATG
                     ELIGDLQAAGERVIVATGGRGGKGNAHFVSSTHRSPRFAQPGEPGEERELDIELRLLA
                     DVGIIGLPNAGKSTLISVISSVRPKIADYPFTTLVPNLGVVGYDDKSFVVADIPGLIE
                     GAHEGHGLGHKFLRHVTRTTLLIHLIDGSQIDPDDPLKDWRTVNDELALFDPALAQKP
                     QIVVINKIDLAEAREHALAFGKEIPERYRPLYLISAATTEGVQALIQTVGRKIEALKQ
                     TETRHENAGI"
     gene            2480..3616
                     /gene="proB"
                     /locus_tag="VFY96_02210"
     CDS             2480..3616
                     /gene="proB"
                     /locus_tag="VFY96_02210"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX5605295.1"
                     /translation="MKTLEYKNDILRRTRRVVVKVGSQILSSREGIEESRVRGLVREL
                     ADLHSAKKELVIVSSGAVAAGMTRLGRTERPKTIPEKQALAAVGQIKLMALYERHFSK
                     FDKSVAQVLLTHEDLANRQRYINAKHTFHMLLQSSIIPIVNENDTVAVEEMKFGDNDH
                     LSALVATLLGADLLVILSDVQGVYDRDPRAYAHAQLIALIEDIKGTKVSIEGSNRSVL
                     GTGGIVTKIAAAEEAAAGGIPTIITSGLGAGAIERVFNPKEQTGTLILADQNRLRHRK
                     HWIAYNLKPAGDVVVDSGAHEAVVHKGKSLLPSGIKEIRGTFGVGECVRCLDLNGQEF
                     ARGLVNYSAQELNQIKGLHTSKIEKILGYKAYDEIIHRDDLVVL"
     gene            3627..4892
                     /locus_tag="VFY96_02215"
     CDS             3627..4892
                     /locus_tag="VFY96_02215"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEX5605296.1"
                     /translation="MQNSSLKEEALRLGTSAKSAAREVAPLSAAEKNRALLAMADELE
                     RRSPLLISENQKDLESAKTAGLSNAVLDRIALNAGRVASMARGLRDVAALPDPVHEVI
                     KMWRRPNGLQVGRMRIPLGVIGIIYEARPNVTADAAALCLKSGNAVILRGGSEAHHSN
                     QAIGSALRDACVGTRVPQDAVQMVGRKDHALVNELLQLEDYIDLIIPRGGEDLIRAVV
                     ANSKIPVIKHYKGVCHVYVEADASLEMAERICLNAKVQRPSVCNAMETLLVHEAIAAK
                     FLPPMIAKFQAAGVEIRGCARTSALVSGVVPAREPDWSEEYLDLILAVRVVRDLDEAI
                     AHIERYGSEHTESIITASYQKAREFVERVNSSAVLVNASTRFNDGGELGLGAEIGIST
                     SKIHAFGPMGLEELTTTKFIVFGDGQIRE"
     gene            5027..6097
                     /gene="nadD"
                     /locus_tag="VFY96_02220"
     CDS             5027..6097
                     /gene="nadD"
                     /locus_tag="VFY96_02220"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX5605297.1"
                     /translation="MKIGLFGGTFDPIHWGHLRSAEEVAETYGLDRVYFIPASVPPFK
                     RAQTSTPPADRLQMVRLAVARNPRFRASTVEISRPGVSYSIDTIRYFGARKRPEDALY
                     FIIGLDAFREISKWKDFAEIFPLCNFVVTSRPGSKESDPLKGTGVAVKKLFCYDLKSR
                     NYRHRSGTRIFFIELTDIAISASEIRALVKRGKSIRYLVPSVVEQYIKQRHLYVDGAQ
                     SVTASNEISSWDKALLLTRLALEKKAYDLVVLDVHALTSIADYFILCSGRSDRQVQSI
                     AQGLEENAADDGVKPYAVEGTQRGHWVLMDFSDVIVHVFYEPVREFYDLDGLWSHAPR
                     AALPEAYTKLIEQFQIANEQLS"
     gene            6173..7702
                     /gene="gpmI"
                     /locus_tag="VFY96_02225"
     CDS             6173..7702
                     /gene="gpmI"
                     /locus_tag="VFY96_02225"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX5605298.1"
                     /translation="MQPVILLILDGFGINPRKDGNAIANASTPNLDKLLRDYPNSHMS
                     MSGTDVGLPAGQMGNSEVGHMILGAGRIVYQDLTLIHKDIDEGTFFTNPVLLDALRKT
                     KAKSDRLHLMGLLGDGGVHSHQSHMEALIEMARREKLDKIFLHLFLDGRDTPPHSAEQ
                     FVLALNEKLKSFPNARIATVCGRYYAMDRDKRWDRVEKAYQCLTEGVGNKAGSALEAI
                     RKSYQEKVTDEFVLPTVIEGVDGLIRDGDGVIFFNFRADRARELTRVFTEEGFSEFPR
                     HRQIRLASYTTMTRYDETFKLPAAYARREIRRILGELASQAGIRQLRIAETEKYAHVT
                     YFFNGGEEKKFPGEERILIPSPKDVPTYDLKPEMSARPVTEALTQFLREQDAGLVIAN
                     YANADMVGHTGNFEASVKAVEVIDECLGKVVDAALSKRGKVIITADHGNIEQLIDYDT
                     GMPHTAHTTNPVPVILVDDEQRNAHLNQGTGVDIAPTLLKLLRQPQPQEMTGRSLIRG
                     S"
     gene            7773..>8508
                     /locus_tag="VFY96_02230"
     CDS             7773..>8508
                     /locus_tag="VFY96_02230"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX5605299.1"
                     /translation="MDFCESRSFADRFDWVVDWLYPPRCRACSLRIHGRDAEYFCADC
                     WAHIRLISHPLCNWCGRPFPDAAGDDHTCGACLSRPPRFAAARAWACYPREEIEEDPV
                     RQVVHKFKYGRKVSLGKPLGRLMALGCREFLDGCRFDLIVPVPLHPKRLRWRAFNQSL
                     LLAREISRAYRIPADPFVLRRDRATPPQTQLPEDERRRNVRSAFSLNPDRSVDGNSIL
                     LVDDVYTSGATVNECSRVLKRGGASEV"
BASE COUNT         1903 a         2261 c         2521 g         1823 t
ORIGIN      
        1 tcctggtttg tgccgcgacg aacgcagctt cggttgtgat gggttttccg tgtcagccaa
       61 gtcgtcgatt tcgattaagc gctgtaagat tttgacaaac ttcggacccg tcgaccgcaa
      121 ctcttcaagg tggtggaaag aaagactatt caaaaactct tcgccgttcc gcgttagctt
      181 taccagcacc tgacgccgat catcggcggc gcgcaaacgg catacaaagc cccgttccac
      241 taggcgatcg ataagctcca ccgtgctgtg gtgttgcagt tgcatccgtt cggccagagc
      301 tccgaccgtg ggcttcagtt cgttgggcaa tcccttgatc gcgagcaaca gctgatgctg
      361 ctgcggctcg atgccggctt tccgtgcggc gttctcgctg aagcgcagaa agcgccggat
      421 ctggtagcgc aattccgcca tcgcttggaa atctgggatt ttggttcgct ttgccatatc
      481 gcgcttcgac acaatcgtag cacgatatat tatggcggaa ctacccgtgt caaggatagc
      541 gcgtcaatta ttttaccgac cccgctcttg cgcgctcggg cctacgattt ttgtggccga
      601 ttccgaacac gccggtaagt gaaccgttcg acggcatgcg gaatcaatcc attctaagct
      661 atgctaaagt cgtgatgttt ggacgatggc gcttcggcgg tttagttgac tttgctgggg
      721 ttttccttta aaaatttagt ttcgacaaaa tcgtatgaaa tatgcagtaa ttagaaccgg
      781 cggcaagcag taccgcgttt ccgaaggcga tatcgtcaaa gtcgaaaaac tcgccggcga
      841 agttggcgcc aaagtggtcc tcgacaatgt tttatttatc ggcggcaatg acgaggtcaa
      901 agttggtgcg ccgctggtcg ccaacgccaa agtcaccggc gagattgtgg gacaggggcg
      961 cgccaaaaaa atcatcgtct ttaaaaagaa acgacgcaag agctacagcc gccagcatgg
     1021 tcacaggcaa tatcagacgg ctttgaaaat taccgcaatc gaagcctgag gcaggagcag
     1081 attatggcgc ataagaaggc gggtggcagt tcccgtaacg gacgcgacag tcaaggccaa
     1141 cggcgtgggg taaaggtttt cgggggcgaa tccgtgcgcg cgggaaatat cctggtgcgc
     1201 cagctcggaa cccgtattca tccggggaaa aatgttggca tggggcgcga ttatactctg
     1261 tttgcgttga tcgacggggt cgtgcgctac gagcacatgg acaagcaccg caagcgcgtt
     1321 gcagttctgc cagctcagtg aaattgagca gcgcttaacg aaagcccctc ttcggggctt
     1381 tttttttgcc cggcggaaga gagctaacga ataggtgaag aacagtcggt cgtgagaccg
     1441 gaccggccac tgaatacttt tcgcatgaaa ttcattgacg aagtcaaaat tcgcgtgatc
     1501 ggcggtgacg gggggcaagg ctgtgtcagt tttcggcgcg agaaattcgt cccgcgcggc
     1561 ggtcccagcg gcggcaatgg cggcaacggc ggtgatgtga tcgccgttgc ggatcctcag
     1621 ctcacgacgt tgctcgatct tcgttatcag aagctctaca aagctggccg cgggcaacat
     1681 ggcatgggca gcgactgtca cgggcaccgg ggcgatgacc gcatcatcaa agttccggtc
     1741 ggaaccatcg tccgcgatgg cgcaacgggt gagctgatcg gtgatttgca agccgccggt
     1801 gagcgagtca tcgtggcgac cggcggccga ggcggcaagg gcaacgcgca ctttgtctca
     1861 tcgacccatc gcagtccgcg gtttgctcaa cccggcgagc ccggagagga gcgtgagctg
     1921 gatatcgagc tgcggttatt ggcggacgtt ggcatcatcg gcttgcccaa cgccggcaaa
     1981 tcaactttga tttctgtgat ctcgtcagtg cgtccgaaga tcgccgatta tccattcacc
     2041 acgctggtgc cgaatttggg cgtcgtcgga tacgatgaca aaagctttgt cgttgccgat
     2101 attccgggtt tgatcgaagg cgcccatgag ggccacggct tgggacacaa attccttcgg
     2161 catgtcacgc ggacgacctt attgattcat ttgatcgatg gatcacaaat cgaccctgac
     2221 gatccgctga aggactggcg aaccgtcaat gatgagctgg cgctttttga tccggcgttg
     2281 gcgcagaagc cacagatcgt ggtcataaat aaaatcgatt tagccgaagc tcgagagcac
     2341 gccctggcgt ttggaaaaga aattccggag cgttatcgcc cgctatattt gatctcggcg
     2401 gcaacgacgg agggcgttca ggcactgatt caaaccgttg gccgaaaaat cgaagcgctc
     2461 aagcaaaccg agacccgtca tgaaaacgct ggaatataaa aacgatatct tacgccggac
     2521 gcgtcgtgtc gtcgtcaagg tcggcagcca gattctttca tcgcgagagg gtatcgaaga
     2581 atccagggtg cggggcttgg tgcgcgagtt ggcggattta cattcggcaa aaaaggaact
     2641 ggtcatcgtt agctccggcg ccgtggcagc gggcatgacg cgccttggcc gcaccgagcg
     2701 gccgaagacg attccggaaa agcaggcgct cgccgccgtc ggtcaaatca aactgatggc
     2761 gctctatgaa cgccacttct cgaagttcga caaaagcgtg gcgcaagtat tgttgacgca
     2821 cgaagatctg gccaatcggc agcgctacat caacgctaag catacgtttc acatgctctt
     2881 gcaatcatcg atcattccga tcgtgaacga gaacgacacg gtggccgtgg aagaaatgaa
     2941 attcggcgac aacgatcatc tctcagcgct ggtggcgacc ttgctcggcg cggatctgtt
     3001 ggtgattctg agcgatgtac aaggcgttta cgatcgcgat ccgcgcgcgt acgctcatgc
     3061 gcagttgatc gcattaatcg aagatatcaa gggaacgaag gtcagcattg aaggatcgaa
     3121 ccgtagtgtt cttggtacgg gcggcatcgt gaccaaaatc gccgccgccg aggaagccgc
     3181 tgccgggggc attccaacga tcattaccag cgggctcggt gccggtgcca tcgagagagt
     3241 gttcaacccc aaagagcaga ccggtacttt gatccttgct gaccaaaatc gcttgaggca
     3301 ccgcaagcat tggatcgcct acaatctcaa accggcgggc gatgtggtgg tcgattccgg
     3361 agctcacgaa gccgtggtgc acaagggaaa gagcttgctg ccttccggta tcaaagaaat
     3421 caggggtacc tttggggtcg gcgagtgcgt gcgctgcctc gatctgaacg gtcaagagtt
     3481 tgcgcgcggg ctggtgaact acagcgcgca ggagttgaat cagatcaagg gattgcatac
     3541 aagcaagatt gaaaaaattc ttggctacaa agcctacgat gagatcatcc atcgcgatga
     3601 tctcgttgtg ctgtaggcgg ccgatcatgc aaaactcttc actgaaagaa gaagcgcttc
     3661 gccttggcac aagcgccaaa tctgcggcgc gcgaggtggc gccactttcg gctgctgaaa
     3721 agaatcgagc actgctggcg atggctgacg agctggaacg acgaagtccg ctcctgatca
     3781 gcgaaaacca gaaggatttg gagtcggcaa agactgccgg ccttagtaac gccgtgctcg
     3841 accgcatcgc gctcaatgcc ggacgggtag cgtcaatggc gaggggattg cgggatgtgg
     3901 ctgccttacc ggatccggtt cacgaagtga tcaagatgtg gcggcggccg aatgggcttc
     3961 aggtcggacg gatgcgcatt ccgctcggtg ttatcggcat catctatgaa gcgcgcccga
     4021 acgtgaccgc cgacgcggcc gcgctctgct tgaagtccgg caacgccgtg attctgcgcg
     4081 gcggcagcga agcgcatcac tcgaatcagg cgatcggcag cgcgctgcgt gatgcctgcg
     4141 tcggcacgcg cgtgccgcag gatgccgtac agatggtagg gcgcaaggat catgcgctgg
     4201 ttaacgaatt gctccagctc gaggactaca tcgatctgat cattccgcgc ggcggcgagg
     4261 atctcatccg cgccgtggtt gcgaattcca aaatcccggt gatcaagcac tacaaaggag
     4321 tgtgccacgt ttacgtcgag gcggacgcat cgctggaaat ggcagaacgg atttgcttga
     4381 acgccaaagt gcagcggccg tcggtctgta acgccatgga aaccttactg gtgcacgaag
     4441 cgattgcggc gaagtttttg ccgcccatga tcgccaaatt tcaagctgcc ggggtggaga
     4501 ttcgcggctg cgcacgaacc tccgccctgg tctcgggagt cgtgcccgca cgcgaacccg
     4561 attggagcga agagtatctc gatttgattc tcgccgtacg cgtcgtgcgg gacctcgacg
     4621 aggcaattgc ccacatcgag cgctacggct cggagcatac ggaaagcatc attacggcga
     4681 gctatcagaa agcgcgcgag tttgtcgaac gggtcaactc gtcggccgta ctggtcaacg
     4741 cgtcgacccg gttcaacgac ggcggcgagc tcggcttggg cgcggagatc ggcatcagca
     4801 cgagtaaaat ccacgcgttc ggccccatgg gtttggagga gctgacgacc accaagttca
     4861 tcgtttttgg cgatgggcaa atccgggaat aggcgttaag catgaagagg agaggtgagg
     4921 gcatggagga gcgcgcgctg cggattctgt tatttcctga ttttcccatc agctgtgccg
     4981 cagtagtcgt ttttgcgtat tgcgccatcc ctcgagcagc cgcggcatga agatcggcct
     5041 cttcggcggc actttcgatc cgatccactg gggacatttg cggagcgcgg aggaggtagc
     5101 cgaaacctac gggctcgatc gggtttattt tattccagca tccgttcctc cattcaagcg
     5161 agcgcagacg tcaacccctc cagccgatcg cttgcaaatg gtgcgtttgg cagtagcccg
     5221 aaacccgcgg ttccgagcgt ccaccgtgga aatttcgcgc ccgggtgttt cctattccat
     5281 tgatacgata cggtactttg gcgcacggaa acggcctgag gacgcgcttt acttcattat
     5341 tgggctcgac gcgtttcgcg aaatctcgaa gtggaaagat tttgcggaaa tttttccact
     5401 ctgtaatttc gttgtcacat cacggccggg gagcaaagaa agtgatccgc tcaaaggaac
     5461 cggcgttgct gtgaaaaaac tgttttgtta tgatctgaaa agcaggaact acaggcatcg
     5521 aagtggtaca cggatttttt ttattgagct cacagatatt gcgatctcgg catcagaaat
     5581 tcgcgcgctg gtcaaacgcg gaaagtcgat ccgctattta gtgccgtctg tggtcgaaca
     5641 atacatcaag cagcgccatt tgtacgtcga cggagctcaa tcggtgaccg cctcgaacga
     5701 gatcagttcc tgggataaag ctctactttt gacccgcctc gctctcgaaa agaaagccta
     5761 cgatctggtg gtcttggacg tacacgcgct gacgtcgatc gcggactatt tcatcttgtg
     5821 ctcagggcgc tcggatcgac aggtgcagag catcgcgcaa ggcctggaag agaacgccgc
     5881 agacgacggg gtgaaaccct atgcggtcga aggcacccag cgaggccatt gggtactgat
     5941 ggatttttcc gatgtgatcg tgcacgtgtt ctacgaaccg gtgcgcgaat tctacgatct
     6001 cgatggcctg tggagtcacg cgccgagagc cgcgcttccc gaagcttaca ccaagctcat
     6061 cgagcagttt cagatcgcca acgaacagtt gtcctgagcc taaatgacgc ttcctcgctc
     6121 gatcggagcg ggaatcctga tgccgtcgcc cggcgagaaa ggcttcgacc caatgcaacc
     6181 cgtgattctt ctcatccttg acggtttcgg tatcaatcca cgcaaagacg gcaacgccat
     6241 tgccaacgca tctacgccca acctcgataa gctcctgcga gattatccga actcgcatat
     6301 gtcgatgtct ggtacggatg tcggtttgcc ggccgggcaa atgggcaatt ccgaagtcgg
     6361 ccatatgatt ctcggcgccg gacggattgt ttatcaggat ttgaccctga tccacaaaga
     6421 tatcgacgaa gggacttttt ttacgaatcc cgttcttctc gacgcgctac gcaaaaccaa
     6481 agcaaaaagc gatcggctcc atctgatggg gcttctgggc gacggcggcg tgcatagcca
     6541 ccagagccat atggaggcgc tgatcgagat ggcgcggcgc gagaaactcg ataaaatctt
     6601 tttgcatttg tttctggacg gccgggatac gccaccgcac agcgcggaac aatttgttct
     6661 cgctctcaac gagaagctca aaagttttcc caatgctaga atcgccacgg tttgcgggcg
     6721 ctactatgcg atggatcgtg ataagcgttg ggatcgagtc gagaaagcct atcagtgctt
     6781 gaccgagggt gtgggcaaca aagccggttc ggcgctggaa gcgattcgca agagctatca
     6841 ggaaaaggtc acggatgaat ttgttttgcc gacggttatt gaaggcgtcg acggtttgat
     6901 tcgcgacggc gacggggtga tcttttttaa tttccgggcg gatcgggcgc gcgaattaac
     6961 ccgggtattt accgaggagg gattcagcga attcccccgg caccgacaaa tacggctcgc
     7021 cagctacaca accatgaccc gctatgacga gactttcaaa ttgccggcgg cgtatgcgcg
     7081 gcgggaaatc agaagaatcc tgggagaact cgcgagccag gccggcattc ggcagctgcg
     7141 catcgccgaa accgagaagt acgcgcacgt gacctatttt tttaatggcg gcgaagaaaa
     7201 gaaattcccc ggcgaggagc ggattctcat cccttcgcca aaagatgtgc cgacctacga
     7261 tctcaaaccg gaaatgagcg ctcggcccgt gacggaagcg ctgacgcaat ttctgcgcga
     7321 gcaagacgct ggcctggtga tcgcgaatta cgcgaatgcc gatatggtgg gccatacggg
     7381 aaatttcgaa gcgtccgtga aagcggtgga agtgatcgac gaatgcctcg gcaaagtcgt
     7441 cgatgcggcg cttagcaaaa ggggaaaagt gatcattacc gccgatcacg gcaatatcga
     7501 gcaattgatc gactatgaca cgggaatgcc gcacacggcg cacacgacca atccagtgcc
     7561 ggtgatcctg gtcgacgacg agcaaagaaa cgctcacctc aatcaaggga caggcgtcga
     7621 tatcgccccg accttgctga aacttttgcg gcaaccgcag ccgcaagaaa tgaccggccg
     7681 atcgttgatc cgcggctcgt aacaatctcg ttatcagccg cttcattccc gactttcgcg
     7741 ccaaccactg gatttgctag agtcgcttgc gtatggattt ttgcgagagc cgttcgtttg
     7801 cggatcgatt cgactgggtc gtcgattggc tctatccgcc gcgctgccgc gcctgcagcc
     7861 tcaggatcca tggccgtgat gctgaatatt tttgtgccga ttgttgggcg cacatccgtc
     7921 tcatttctca tcccctgtgc aactggtgcg gccgcccgtt tccggacgct gcgggagacg
     7981 atcacacctg tggtgcttgt ctatctcggc cgccccgatt cgcggcggcg cgcgcgtggg
     8041 cctgctaccc gcgcgaggaa attgaagaag atcctgtgcg acaggtggtg cacaaattca
     8101 aatatggccg caaagtttct ttggggaagc ctctgggacg attgatggcg ctgggctgcc
     8161 gcgaatttct cgacggctgt cgtttcgatt tgattgtgcc cgtgccactt catccaaaaa
     8221 gattgcgctg gcgggctttt aatcagtcac tgctactcgc gcgtgagatc agtcgggctt
     8281 accggatacc cgcggatccg tttgtgctcc ggcgcgaccg cgcgacgcca ccgcaaacgc
     8341 aattgccgga ggatgaacgg cgaagaaacg tgcgcagcgc tttttctctc aaccccgatc
     8401 gctctgtgga tggcaacagc atattgctgg tggatgacgt gtacacttcg ggtgccacgg
     8461 taaatgaatg tagccgcgtc ttgaagcggg gtggcgcgag cgaagtct
//
LOCUS       DASULZ010000109         2017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_12437, whole genome shotgun sequence.
ACCESSION   DASULZ010000109 DASULZ010000000
VERSION     DASULZ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12437"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..616
                     /locus_tag="VFY96_02235"
     CDS             128..616
                     /locus_tag="VFY96_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605300.1"
                     /translation="MTAQKIKVADEVWIATGLLHRENPKREDFTVAEIVERVRRENIF
                     GELRPGIQVHAYLHCVANRPPNPGRYRMLYATGKKTRRLFRESDDFDPQRRDGKVKPE
                     KENIPVKYHALLDWYGEKFVGKRKGLKETDPILGLCGLGKEIWVGEDPDEYVSRVRGG
                     WE"
     gene            613..1035
                     /locus_tag="VFY96_02240"
     CDS             613..1035
                     /locus_tag="VFY96_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX5605301.1"
                     /translation="MSRIFWDTNLFIYLIEEFGDLSERVISLRKRMVERRDELYTSAL
                     TLGEILIKPLEAGDEVLARRYEAALLQGAVVIPFDVEPAQLYATIRKDRTIRPPDAIQ
                     LACAAHARVDLFITNDERLSMKSIPRIQFVSSLQRAFL"
     gene            1408..>2017
                     /locus_tag="VFY96_02245"
     CDS             1408..>2017
                     /locus_tag="VFY96_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009950247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HEX5605302.1"
                     /translation="MAQEKINATRRQTNLPNESDKYLSKREELRLAEIELMRQRERVA
                     ELRRGLPQGAAIQDYAFEEGPANLDAGDTPIRTVRLSELFSAPNRSLVVYQFMFGKKN
                     TTPCPMCTLWIDGWNGVAHHLAQNVDFAIVAAADPMALRAHARTRGWHRLRLLSCGAN
                     TFKYDLGSEDREGNQDSTISVFTRDTDGTVRHFYTAHPSLSSD"
BASE COUNT          491 a          506 c          549 g          471 t
ORIGIN      
        1 tgagttttcc aagccaggaa gcgagaagtt cccaggtact tgagaacggt tgttatcgca
       61 ggcctcttga catatcttcc tttggatata gattttacaa ggtaaacatc ataatcagag
      121 gaataaaatg accgcacaaa agatcaaagt tgccgatgag gtttggatag cgaccggtct
      181 ccttcaccga gagaatccaa aacgcgagga tttcactgtt gctgaaatcg tcgagcgggt
      241 acggcgcgaa aacatattcg gtgagctaag accagggatt caagtgcatg cctaccttca
      301 ttgcgtcgcc aaccgtccgc cgaacccggg gcgttatcgg atgctttatg ccaccggcaa
      361 aaaaacccgc cgtctgtttc gggaaagtga tgactttgat ccgcagcgtc gcgatgggaa
      421 ggtaaagcct gaaaaagaaa acattcctgt caagtatcac gcgctactgg actggtacgg
      481 tgaaaaattt gttggcaaga gaaagggtct caaagaaacc gaccccatcc ttgggctttg
      541 tgggttaggg aaagagatct gggttggcga agatcccgat gaatatgtca gccgggttcg
      601 cggaggatgg gagtgagccg aattttttgg gacacgaatc tgttcatcta tctgatcgag
      661 gaatttggcg atctgtcgga gcgtgtcatc agcttgcgca aacgcatggt cgagcgccgg
      721 gacgagctct acacctctgc gctgacgtta ggagagatcc tgatcaagcc gcttgaggcg
      781 ggtgacgagg tcctggcgcg ccgctatgaa gccgcattgc tacaaggcgc ggttgtcatt
      841 ccttttgatg tagaaccggc ccagctctat gcaactattc gtaaggatcg taccattcgt
      901 ccccccgacg cgattcagct tgcgtgtgcg gcgcatgcgc gtgtcgatct gtttatcacg
      961 aatgacgagc gtttaagcat gaaatcgatt ccgcgcatcc aattcgtgag ctcattacaa
     1021 cgggcattct tgtgagtcat tttgatgatc ctccggatca aatatcaatt tcataaccgt
     1081 tgtctttcga tacgaaaaag gttgtatttg gcatatcact caccgctgcc agcaaaagca
     1141 attctactgc agtttgccga cgccggtatt tgcattgggt ttttcaagcc cgaagcgaga
     1201 agttgtcgca agattctttt ggttcatact tcagattccc attcgatctg acatacttta
     1261 ataacagtcg caaaaatttt ccgcacgacg gtcctaaaag cgctcgctca ttcgtcgcgc
     1321 tattgagtga ggcatcggtg tctggcgggc gaatgcagca ggaatgtctc caaacttttt
     1381 ggcgaaaagc ttgaggaggt atgaaccatg gcacaggaga aaatcaatgc gacgcgtcga
     1441 cagaccaatt tgccgaacga atccgacaaa tatctatcca agcgagaaga acttcgactg
     1501 gccgaaatcg agctgatgcg ccagcgagag cgagtcgcag aacttcgccg cgggctcccg
     1561 caaggcgcgg cgattcagga ttatgcattc gaagagggtc cggcgaacct cgatgccggc
     1621 gacacgccga tccgcaccgt ccgcctgagc gaactgttca gcgcgcctaa tcgttcgttg
     1681 gtcgtctatc agtttatgtt cggaaagaag aacaccacgc cgtgcccgat gtgcacgctc
     1741 tggatcgatg gttggaatgg cgtcgctcat catctggctc agaacgtcga ctttgcgatc
     1801 gtggccgcgg ccgatccgat ggcgttgcga gcacacgcgc gtacgcgcgg ctggcacagg
     1861 cttaggctgc tgagttgcgg cgccaacacg ttcaaatacg atttggggag cgaagaccgc
     1921 gaggggaatc aggattcgac catctccgtt ttcactcgag atactgacgg aaccgttcgt
     1981 cacttctaca cagctcatcc gtctctctcg agcgaca
//
LOCUS       DASULZ010000110         2133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1230017, whole genome shotgun sequence.
ACCESSION   DASULZ010000110 DASULZ010000000
VERSION     DASULZ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230017"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..1165)
                     /locus_tag="VFY96_02250"
     CDS             complement(170..1165)
                     /locus_tag="VFY96_02250"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605303.1"
                     /translation="MKISRQNNFQSVVRAVRLIPISFLLFLVAAHSAEIPKERIAFAY
                     ASISPSMSAVWMAKEIGAFDRNGLNAELVYISSGATTIQALVGGSVQAALGASNAVVA
                     AALKGAPIIAVASNTSRPGMQLWVQPEIQRADQLQGKTLAITRFGSTSDFVTRLVLRK
                     LNLENKTELRQFGGVVEADLGFRARQAEGRVSSQAPGPKAKMLVDAAELGIPFSMNLL
                     AVSNDYLSKSPKSVEAIVRAYIEGVAALKTRKAQALKVFDKYMGQRGGSAEMHHEFIM
                     RYLDSVPRIDPAAVETVLEMVGVKGTPKSKLYDNSIIDRLVHEGFTEKLYKGAKS"
     gene            complement(1162..1866)
                     /locus_tag="VFY96_02255"
     CDS             complement(1162..1866)
                     /locus_tag="VFY96_02255"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX5605304.1"
                     /translation="MDANGWSKAFGELARQTMTSPELERYFSIPITKPRAQLILQQLS
                     LYVRHRRDCWAYVSGNSPVLAVKQKILAHEYEEVIRDQHSEHGHLDLIVRQGKCIGLN
                     GDDILNTKPIFSTTATLYAWGWMCKKKHWLEGLAAMTMTEWNQDDRLLGDLGGGHAYR
                     MGQRWIEQLGITWDDLPNWKAHREADRGHVDMFLPVWEEFGKGDNEKMILEAAKESEE
                     LYRVYQAGLADAMERM"
BASE COUNT          447 a          646 c          531 g          509 t
ORIGIN      
        1 gagacatgcg accagcattc gcgccgatgg cgtacgaaca agctctgctg cttgaccatg
       61 accgccgcgc ggggcttgtt catcttgatg gaaaaataat attccagctc cggcgatcgg
      121 atataatcgc gcaccagctc accgagacat tcgcgccact gcttttccgt catgactttg
      181 ctcctttgta aagcttttcg gtgaaccctt cgtgaaccaa ccggtcaatg atcgaattat
      241 cgtaaagttt gctcttcggc gtgcctttca cgcccaccat ttcgagcacc gtctccaccg
      301 ccgccggatc gatgcgtgga accgaatcga gatatctcat aatgaattcg tggtgcatct
      361 cggcgctgcc gccgcgctgt cccatgtatt tgtcgaatac cttgagtgcc tgagctttcc
      421 tggtcttcag tgcggcaacg ccttcgatgt aagcccggac aattgcttcg acggacttcg
      481 gcgatttcga gagatagtcg ttgctcacgg ctaacagatt catcgaaaac ggaatgccaa
      541 gctccgccgc atcgaccagc atcttcgcct tcggcccggg cgcctgcgac gagacgcgcc
      601 cttccgcttg ccgcgcgcga aaaccgagat cggcttcgac cacgccgccg aactggcgca
      661 gctccgtttt gttttcaaga ttcagttttc gcaaaaccag ccgcgtcacg aagtcgctgg
      721 tggagccgaa ccgggtaatc gcgagcgttt tgccttgcag ctgatctgcg cgttgaatct
      781 ccggctgtac ccaaagctgc atgcccgggc ggctggtatt gctcgcgacg gcaataatgg
      841 gcgcgccttt cagcgccgcg gcaacaaccg cattgctcgc cccaagcgcc gcttgaacgc
      901 tgccgccgac caatgcctgg atcgtcgtcg cgcctgagct gatgtaaacc agctccgcat
      961 tcaaaccgtt tctgtcgaag gcgccaatct ccttggccat ccagacggcc gacatgctgg
     1021 gactaatgga agcataggcg aacgcgattc tttctttggg gatttcagcg gaatgagcgg
     1081 cgacaagaaa taataagaac gaaataggaa ttagtctgac ggcgcgcacg acgctttgaa
     1141 agttattttg ccgagatatt ttcacatccg ttccatcgca tccgcgaggc ctgcctggta
     1201 aacgcgataa agctcctccg attccttcgc cgcctctaga atcatttttt cgttatcacc
     1261 tttgccgaac tcctcccaca cgggcagaaa catatcgacg tgccctctgt cggcctcgcg
     1321 atgcgctttc cagtttggca gatcatccca ggtgatgccc agctgctcga tccagcgttg
     1381 gcccatccga tacgcatgtc cgccgcccaa atctcccagc agtcgatcgt cctgattcca
     1441 ttccgtcatc gtcatcgccg ccagaccttc gagccagtgc tttttcttgc acatccagcc
     1501 ccacgcgtag agggtcgccg tggtgctgaa aatcggtttg gtgttcaaaa tatcatcgcc
     1561 attgagaccg atgcattttc cttgacgaac aatcagatcc aaatggccgt gctccgagtg
     1621 ttgatcacgg atcacttctt catactcgtg tgcgaggatc ttttgcttca cggcgagaac
     1681 gggcgagtta cccgacacat acgcccaaca atcgcgccgg tgacggacgt acagcgatag
     1741 ctgctgcaga atgagttgcg cccgcggctt tgtaattgga atcgaaaaat aacgctcgag
     1801 ctcgggcgac gtcatggttt gccgcgctaa ttcgccgaac gccttcgacc acccatttgc
     1861 atccatgtaa gattctcctg ctcagcttaa ttttttggag ctcccccttc ggaaaagcca
     1921 gagcgagggg atttctcgaa aatcctccct acgctccttt gccaaaggag gggtttgaat
     1981 acacctgagc cggcgtcgtg cgcttggctt aaacaaattt ctccatttcc ttggagatgc
     2041 cgtagcggaa aatcttcagc agctccatcg actctttcgc ggcccgataa accaactgtt
     2101 ctttctcacc ggtggcgaac ctttcaaggt cct
//
LOCUS       DASULZ010000111         4058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_418470, whole genome shotgun sequence.
ACCESSION   DASULZ010000111 DASULZ010000000
VERSION     DASULZ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418470"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1824)
                     /locus_tag="VFY96_02260"
     CDS             complement(<1..1824)
                     /locus_tag="VFY96_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX5605305.1"
                     /translation="MPDSSARPTEYEFIVIGSGAGGGTVAARLAEAGHSVLVLEAGGD
                     PLQLIDERLPEDYQVPAFHAFASENAALRWDFFVRHYADDERQRRDPKYTSIKKGVLY
                     PRAGTLGGCTAHHAMIFVYPNNSDWDYIADITGDSSWRAAHMRRYFERLENCRHRPVW
                     RFVQKLTRINPTLHGFAGWLPTERAHPPKEAWRDREMMDVIEASAFAAIAQLDQPLKR
                     LFWFLRAKGDPNDWRSVRRNAFGIRYLPLTTSDHQRFGSRERLLDVAARYPEKLHIEL
                     DALASRIIFDADHRAVGVEYLKGQNLYRACDQTTGDSGQRRHAGATREVILCGGAFNT
                     PQLLMLSGVGPKSELARHGINVRIDLPGVGANLQDRYEVSVVNRMRDDWECMAGAEFV
                     RGDRQFVEWQKHRKGIYATNGGLLTVIKNSRDQKALPDLFCMSLLARFEGYFPGYSKL
                     ISGSRDFFSWVILKAHTQNRAGTVKLRSADPRDPPDINFRYFEGDGADEDLRAVVDGI
                     KFVRAISAELKKEKLIAKEELPGDNVQSDDDLKEFVRSNAWGHHASCSCPIGARENGG
                     VLSSDFRVHGTRGLRVVDASVFPRIPGYFIVSAVYMIGEKAA"
     gene            complement(1843..>4058)
                     /locus_tag="VFY96_02265"
     CDS             complement(1843..>4058)
                     /locus_tag="VFY96_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605306.1"
                     /translation="ELTANEVKGRNNWIVWTGGNDRFWDDLVNRSFGAVDFLKIISSH
                     PKVQHLSRDTRWKYLGLVNEPCFEKPQAARSDRFGLWLDGRSKDCPADPFENEQKYPG
                     VKIGARGKNIPVGSYYGYGTGIVGLRLFPNPDFDETAAKRWNAERFYTDPNYYNDKHL
                     VRPYRVGMSCGFCHVGPSPIKPPADPENPKWENLNSNPGAQYFWVDRILFWNKDEGNF
                     IYQLLHTSLPGTLDTSFISSDYINNPRTMNAVYSVGARLAPALRWGKEKLAGDELKNK
                     QFQDFPQTAALSQFFQKPDTVMTPRVLKDGADSVGLLGALNRVFINIGLFSEEWTLHF
                     NPLVGGKKITPIRIEDAEKNSSYWNATVNQTADVALFFLKTAKPDSLKNAPGGEKYLT
                     GDKATIDRGKLAFANYCARCHSSKVPAAPANVNDSDWSQYWAWTKTDDFKEKMKAIVL
                     ADDFLTDNYLSTERRIPVTLLQTNACSPLATNALGGNIWDNFSSQTYKNLPSAGKMTF
                     YNPIDASPFEYELPAGGRGYTRVPSLTSLWSTAPFLLNNSVGKFNWEPSIESRVDAFN
                     DAIEKMLWPEKRDKDPILGDKVPGFVYRTTATSYIKLAPGFLPDSLHKLLSWNDWLAR
                     LFPWLFSEQGVRIGPIPKGTPVSLLTNIDLETDKLDLATLLVKMKEDLKRVEGQGDTE
                     AAQVLKNLVPDLIKVSKCPDYIVNKGHYFGTTFFKEEPPLSDEDKRALIEFLKTL"
BASE COUNT          856 a         1135 c         1125 g          942 t
ORIGIN      
        1 ggcggccttt tcgccgatca tatacacggc gctcacgatg aagtaaccgg ggattcgcgg
       61 aaatacggag gcgtccacta ctcgcagccc gcgagttcca tgcacccgga aatcgctgct
      121 cagaacgccg ccgttttcgc gcgcaccgat ggggcaggag caagacgcat gatgccccca
      181 ggcattgctg cgcacgaact cctttaaatc gtcgtcgctt tgaacgttat cgcccggcag
      241 ttcttctttg gcgataagct tttctttttt cagctcggcg ctgatcgcgc ggacaaactt
      301 aatgccgtcg accaccgccc gcaagtcctc atcggcgcca tctccttcga agtaacgaaa
      361 gttgatgtcc ggcggatcgc gcggatcggc tgatctgagc ttcacggttc ccgctctgtt
      421 ttgggtatgc gcctttaaga ttacccagct gaaaaaatcg cggcttccgg aaatgagctt
      481 cgagtaacct gggaagtacc cttcgaagcg cgccagcaag ctcatgcaaa acagatccgg
      541 aagcgccttt tgatcgcgcg aattcttgat gaccgtgagc aggcccccgt tggtcgcata
      601 aatgcctttt ctgtgtttct gccattcgac aaattgtcga tcgccccgga cgaactcggc
      661 gcccgccata cattcccaat catctcgcat tcgattgacg acgctgactt catagcggtc
      721 ctgcagattg gcgccgaccc ccggcagatc gatgcgcacg ttaatgccgt gccgcgccaa
      781 ctcgcttttc ggcccgacgc ccgaaagcat tagtagttgc ggtgtgttga aggcgccgcc
      841 gcatagaatc acttctctcg ttgcacctgc gtgccgccgt tgaccggaat ctcccgtcgt
      901 ctgatcgcag gcgcgataca ggttttggcc cttgagatat tccacgccga cagcgcgatg
      961 atcagcgtcg aagatgattc ggctcgccag agcatccagc tcaatatgca gtttctctgg
     1021 ataacgggcg gcgacgtcca agagcctctc gcggctgccg aagcgctggt gatcggaggt
     1081 ggtcagtggc agataacgaa ttccgaaggc attgcggcgc acgctgcgcc aatcgttggg
     1141 atcgcccttc gctctgagaa accagaataa ccgcttgagc ggctgatcga gttgcgcgat
     1201 ggcggcgaac gctgacgctt cgatcacgtc catcatttct cggtcgcgcc atgcttcttt
     1261 cggtggatga gcccgctcgg tgggcagcca gccggcaaag ccgtgcaagg tcggattgat
     1321 gcgtgtgagc ttctggacaa aacgccacac cggacgatga cggcagtttt cgagacgctc
     1381 aaaataacgc cgcatgtgag cggcgcgcca tgaggaatcg ccggtgatgt cggcgatgta
     1441 atcccaatcc gaattgttcg gatagacaaa gatcatggcg tggtgcgcgg tgcagccgcc
     1501 gagggtaccg gcccggggat aaagcacccc cttttttata gacgtatact tcggatcgcg
     1561 cctttggcgc tcatcatcgg catagtggcg cacaaagaaa tcccatcgca aagccgcgtt
     1621 ttccgaggcg aaggcatgga atgccggcac ttgatagtct tcggggagtc gttcgtctat
     1681 tagctgaaga gggtcgccgc cggcctcgag aaccagcacg gagtggccgg cctcggcaag
     1741 tcgggccgcc accgtcccgc cgcccgcccc cgagccgatg acaataaatt cgtactcggt
     1801 cggacgcgcg ctggagtcag gcatcaagtg gctttcggaa cgttagagcg tcttcaaaaa
     1861 ctcgataagc gcccgcttgt cctcatcgct gagcggcggc tcttccttga aaaaggtcgt
     1921 gccgaaatag tgtcccttgt tgacaatata atcgggacac ttgctgacct tgatcagatc
     1981 gggaaccagg tttttgagca cctgagcggc ttccgtgtcg ccttggcctt cgactcgctt
     2041 gagatcttct ttcatcttga ccaagagcgt ggcgagatcg agcttgtcgg tctcgagatc
     2101 gatgttagtg agcaaactga ccggcgtgcc cttgggaatc ggtccgatcc tcacgccttg
     2161 ttcgctgaac agccacggaa acaagcgcgc cagccagtcg ttccagctga gcagtttgtg
     2221 cagcgaatca ggcagaaaac ccggcgcgag cttgatgtag ctcgtagctg tggtgcgata
     2281 aacaaacccc ggcactttgt cgccgagaat cggatcttta tcccgttttt ccggccagag
     2341 catcttctcg atggcatcgt tgaaagcatc gacgcgcgac tctatggaag gctcccagtt
     2401 gaattttccg acggaattgt tcaagagaaa cggcgccgtc gaccataggc tggtcaacga
     2461 cggcactcgc gtatagccac ggccgccggc cggcaattca tattcgaatg ggctagcgtc
     2521 gatgggattg tagaaggtca tcttaccggc cgaaggcaag ttcttgtaag tctgcgacga
     2581 gaagttatcc cagatattgc cgcccagcgc attggtcgcg agcggactac aggcattcgt
     2641 ctgcagcaac gttacgggaa tccggcgctc cgttgaaaga tagttgtccg tgagaaaatc
     2701 atccgcaagc acaatcgctt tcattttttc tttaaagtca tcggttttag tccatgccca
     2761 gtattgactc cagtcggagt cattgacatt cgccggtgca gcgggaacct tgctcgaatg
     2821 acaacgcgcg cagtaattgg cgaaagccaa tttgccgcgg tcgatggtgg ctttatcgcc
     2881 agtcagatac ttctcgccgc ctggagcatt cttcagcgag tcaggttttg ccgtctttaa
     2941 aaagaacagc gccacgtcgg ctgtctggtt caccgtggcg ttccagtagc tggaattttt
     3001 ttccgcgtct tcgatccgaa tcggcgtaat tttttttccg cccaccagtg gattgaaatg
     3061 cagcgtccat tcttcgctga acagaccgat gttgatgaaa actcgattca acgcgccgag
     3121 cagtcccacg gagtctgcac cgtcctttaa cacccgcggc gtcatgaccg tatccggttt
     3181 ttgaaagaac tgagagagcg cggcggtctg gggaaaatct tgaaattgct tgtttttgag
     3241 ttcgtcgccg gcaagttttt ctttgcccca gcggagcgcc ggcgcgagtc gtgcaccaac
     3301 gctgtagact gcgttcatcg tgcgtggatt attaatgtaa tcgctggaaa taaacgaggt
     3361 gtccaatgtc cccggcaagg acgtgtgcag cagctgataa atgaagttgc cttcgtcttt
     3421 attccaaaaa aggatgcggt cgacccaaaa atactgtgcg cctggattgg agttgaggtt
     3481 ttcccatttg ggattctccg gatctgcggg cggcttgatc ggactcgggc cgacgtgaca
     3541 gaagccgcat gacataccga cgcgatacgg ccggactaaa tgcttgtcgt tgtagtagtt
     3601 gggatcggtg tagaaccgtt cggcattcca tctttttgcc gctgtctcat cgaaatccgg
     3661 gttcggaaaa agtcgcaaac cgacgattcc ggtgccatag ccgtaatagg atccgaccgg
     3721 aatatttttt ccccgcgcgc cgatctttac tcctggatac ttctgctcat tctcgaaagg
     3781 atcggcggga caatctttac tgcgaccgtc gagccataga ccgaaacgat cggatctggc
     3841 agcctggggt ttctcaaagc agggttcgtt caccagaccg agatatttcc agcgcgtgtc
     3901 ccggctgaga tgctggacct tgggatgaga cgaaatgatc tttagaaaat ccaccgcgcc
     3961 aaagctccgg ttcacgagat catcccaaaa ccgatcgttc ccgccggtcc acacgatcca
     4021 gttgtttcgg cctttgactt cgttggccgt gagctcta
//
LOCUS       DASULZ010000112         4205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1027191, whole genome shotgun sequence.
ACCESSION   DASULZ010000112 DASULZ010000000
VERSION     DASULZ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027191"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VFY96_02270"
     CDS             <1..623
                     /locus_tag="VFY96_02270"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605307.1"
                     /translation="KTIESLKGAKIAVSSLQAGSTQVLKYILKQRGLIYPRDYNLIRA
                     GGTTLRWAALQTQQVDGAILAEPVSLIAVEKGFANLGEAYKLVPGYQLAGVWIREGWA
                     AKNKDVTLRFLRAFHNALKWLHDNRTEALQVLPKITTLPKQYVQSAWETYTKAIWPRD
                     GRANRQGLQLVIDLMAEDGLLKKPVKPEDIIDDSYLREIGGEFGAK"
     gene            complement(676..1854)
                     /locus_tag="VFY96_02275"
     CDS             complement(676..1854)
                     /locus_tag="VFY96_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX5605308.1"
                     /translation="MRHAEVRGTLTVALLLTLLGAFASTAIAGESSPRAVQTVPGMPP
                     VIDPNNLYSETRPDKLSPVVAKHLHLVYVPNRASNDVWVIDPATLKVIDKYKVGINPQ
                     HVVPSWDLKTLWVNNNAEGRTDGSVTPIDPLTGKPGKNIPVDDPYNMYFTPDGSAAIV
                     VAEAHKRLDFRDAQTMALKYSIATPDCRGINHADFSIDGRYAIFTCEYAQSIAKIDLV
                     DRKVVGYLKFSKGGMPQDVRISPDGKIFYVADMKAEGVFLVDGDKLFEIGHIKTGLGT
                     HGLYPSRDGTKLYVANRGVARVYGPPKGKGSVSVIDFATRKVEATWPIPGGGSPDMGN
                     ISIDGKQLWLSGRYDNSVYVIDTTTGEVKSIPVKTEPHGLTVWPQPGRYSLGHTGNLR
                     "
     gene            complement(1953..>4205)
                     /locus_tag="VFY96_02280"
     CDS             complement(1953..>4205)
                     /locus_tag="VFY96_02280"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5605309.1"
                     /translation="GSAVYGYDLVLPNMLYGKTLFSTRAHARIKRINSEKAKKLPGVV
                     AVVTGEDVPWTHGESVKDKPFLAQGRVRFIGEPVAAVAAEDEDTAQAAVKLLEVEYED
                     LPVYTDPEEACKPGCVAIHEDFDKYRKAEFIVPGAMPNVAEHFKLRTGNVEQGFKESD
                     LVLEERYFVPIIQHAAMEPHSAHAQFDKESGRLTIWVANDAPFRALHEITEALNMPKE
                     KIRFINPLQGGGFGSKGGLKVEPIAVALAFHTNGRPVRVKFNREETFISTLTRHEAVV
                     YSKTGVKKDGTLIAREMTIYWGAGAYAEKSPTVCIRGSLPAPGPYRIPHVKVDGYAVY
                     TNKPVAGSYRGYGIPQGAWAGEQQMDQIARRLGMDPIDIRMKNIFVEGDISYWGERLH
                     AVGLKETLIKATEAIDYGKRSLNPKPGIKIGKGFACIQKPTRSPTTSAAGVRVNAKGE
                     VTVLAGTVEIGQGCNTILSQVAAEELKVPMEKIRMHPLDTDVIPYDASTTSSRSTYHM
                     GNAVRRASIHAREQIMEAARPMLEAEMKEIDYADGKVFLKAQPQQALPIGEVVRRKLG
                     INGEVRGDGSYTYEIGKDLDLETGHSDHASAFYMYATQAAEVAVDEDSGRVRVLRMSA
                     AHDVGKAINPLNCVAQIEGGVVMGIGSALHEEMVVDATGKVRNPSFLDYHLATSLDIP
                     ELISIVVECAEPEGPYGAKGLGEPGLAPTPAAIGNAVADAIGVRIYDLPLKPENVYWA
                     MQKQKKKSAA"
BASE COUNT          858 a         1170 c         1200 g          977 t
ORIGIN      
        1 tcaaaactat cgagtcgctc aaaggcgcaa agatcgcggt gtcgagcctt caagccggct
       61 cgactcaagt gctcaaatat attctcaagc agcgcggatt gatttacccg cgcgactata
      121 atttgattcg cgcgggcggg acaacgctac gttgggcggc gctgcaaacg caacaagtcg
      181 atggggcgat tctggccgag ccggtcagcc tcatcgcagt ggaaaagggt tttgccaatc
      241 tcggcgaggc ctacaagcta gtcccgggat accaactcgc cggcgtgtgg atcagagagg
      301 gttgggcggc gaaaaacaaa gacgtgacgt tgcgattcct aagggcgttc cataacgcac
      361 tcaaatggct gcatgacaat cggaccgaag cgctgcaggt tttgccgaag atcaccacct
      421 tgcctaaaca gtacgtgcag agcgcgtggg aaacctatac caaggcaatt tggccgaggg
      481 acggcagggc caatcgccaa ggactacagc tggtgatcga tctgatggcc gaagatggac
      541 tgctcaagaa gcccgttaag cccgaagaca tcatcgatga ttcttatttg cgggagatcg
      601 gcggcgaatt cggcgcgaaa tagtttcgcc agcgcgcaaa tctccgttct gactggctac
      661 atggacaacc gcaactcagc gcaagtttcc ggtgtgtcct aacgagtatc gtccaggctg
      721 cggccagacc gtgaggccgt gcggttcggt tttaaccgga atacttttta cctcacccgt
      781 tgtcgtgtcg ataacataaa ccgaattgtc ataacgcccg gaaagccata actgcttgcc
      841 atcgatgctg atgtttccca tgtccggact gccgccgccg gggatcggcc atgtcgcttc
      901 cacttttcgc gtggcgaaat cgatcacgct cacgctgcct tttccttttg gcgggccgta
      961 aacacgcgcg acgccgcgat tggcgacata aagtttggtg ccgtcgcggc tggggtagag
     1021 gccatgggtg ccgaggccgg tcttgatatg gccgatctca aagagcttat cgccatcgac
     1081 cagaaaaacg ccttcggctt tcatgtccgc gacatagaat attttgccgt ccggagagat
     1141 gcgcacgtct tgcggcatgc cacctttgga gaatttaagg tagcccacga cctttctatc
     1201 gactaagtcg atcttggcta tgctttgggc gtactcgcag gtgaagatcg cgtaacgtcc
     1261 gtcaatggaa aaatcagcat ggttgatgcc gcggcaatcg ggtgtcgcaa tcgaatactt
     1321 cagcgccatc gtctgcgcgt cgcggaaatc gagccgttta tgcgcttcag caacgacgat
     1381 ggctgcgctg ccgtcgggag taaaatacat gttgtacgga tcgtccacgg gaatattctt
     1441 gcccggcttg cccgtgagcg gatcgatcgg cgtgacactg ccgtcggtgc ggccttcggc
     1501 attattgttg acccagagtg tttttaaatc ccacgagggc acgacgtgct gcgggttgat
     1561 gccgactttg tatttgtcga tgaccttcag cgttgccggg tcgatcaccc aaacgtcgtt
     1621 ggaagctcga ttgggcacat aaacgagatg caaatgcttg gcgacgaccg ggctgagttt
     1681 gtccgggcgc gtttcgctgt agagattgtt cggatcaatc accggcggca tgccgggaac
     1741 ggtttgaacc gcccgcggcg atgattctcc ggcgatggcc gtggacgcga aagcgccgag
     1801 cagggtcaaa agaagagcga cagttaacgt gccgcgaacc tcggcgtgcc gcatgaagtt
     1861 tccctccgac atacgtattg gtcaccggct cagcggtgca gcgagcggtt ggcaaatgtt
     1921 agatgaaaag ctttaggaat tcgagagttt tgttatgcgg cgcttttttt cttttgtttc
     1981 tgcatcgccc agtagacgtt ttcaggtttg agcggaagat cgtagatgcg cacaccaatg
     2041 gcatctgcga cggcattgcc gatggcggcc ggtgtgggtg ccagtccggg ctcgccgagt
     2101 cctttggcgc cgtaggggcc ttcgggttcg gcgcactcga cgacaattga aatcagctct
     2161 ggaatatcca gcgaagtcgc tagatgataa tcgagaaagg atggattgcg cactttgccg
     2221 gtggcgtcca cgaccatttc ttcgtgcaac gccgagccaa tgcccatcac gacaccgcct
     2281 tcgatctgag ccacgcaatt gagcggattg atcgccttgc cgacgtcgtg ggccgccgac
     2341 atgcgcaaaa cgcgaactcg accgctgtct tcgtcaacgg cgacttcggc cgcctgggtc
     2401 gcatacatat aaaacgcgga agcgtgatcg ctatggccgg tttccagatc caagtctttg
     2461 ccgatctcgt aagtgtaaga tccgtcaccg cgcacttcac cattgatgcc aagcttgcgc
     2521 cgcactacct cgccaatcgg cagcgcctgc tgcggctgag ctttcaaaaa taccttgcca
     2581 tccgcgtaat cgatctcttt catctcggct tcgagcatcg gccgtgcggc ttccatgatc
     2641 tgttcgcgcg cgtggatcga tgcgcgccgc accgcattac ccatatgata ggtgctgcgg
     2701 cttgaagtcg tcgacgcgtc gtaggggatc acatccgtat cgagcggatg catgcggatt
     2761 ttttccatcg gcactttgag ctcttcagca gcgacctgag agagaatcgt gttacagccc
     2821 tggccaattt cgacggttcc cgcgagcacg gtcacttcgc ctttggcatt gacccgcact
     2881 ccggcagccg acgtcgtcgg cgagcgggta ggcttctgaa tgcacgcgaa gcccttgccg
     2941 attttaatgc cgggcttcgg gttcaatgat ctcttgccgt aatcgatcgc ctcggtcgcc
     3001 ttaatcagtg tttctttcaa accaaccgca tggagccgct caccccagta ggagatatcg
     3061 ccctcgacga aaatattttt catccggatg tcgatgggat ccatgccgag acggcgcgcg
     3121 atctggtcca tctgctgctc gccggcccag gcgccctggg gaattccata gccgcggtac
     3181 gatccggcga caggcttgtt ggtataaacc gcgtagccgt cgactttgac gtgcggaatg
     3241 cgatacgggc ccggcgcggg taagctacct cggatgcaga ccgtcggact tttttcggcg
     3301 taagcaccgg cgccccaata gatggtcatc tctcgcgcta tcaaggtgcc gtcctttttg
     3361 actccggttt ttgaataaac cacggcttcg tggcgcgtca gcgtcgaaat aaaagtttct
     3421 tcccggttga attttacgcg cacgggtctg ccgttggtgt gaaatgcgag cgccacggca
     3481 atgggctcga ctttcagtcc acctttagag ccgaaaccgc ctccctggag tggattgatg
     3541 aagcggattt tctccttcgg catgttcaag gcttcggtaa tctcatggag cgcgcgaaat
     3601 ggcgcatcgt tggccaccca aatcgtcaaa cgacccgatt ctttatcgaa ctgcgcgtgc
     3661 gcggagtgtg gttccatcgc ggcatgctgg atgatcggaa cgaaatatct ttcttcgagc
     3721 accagatcgg attctttgaa accttgctcg acattccctg tgcggagctt gaagtgttca
     3781 gcaacgttgg gcatcgcgcc cggcacgatg aattcggcct tgcggtattt atcaaaatcc
     3841 tcgtggatcg cgacgcaacc cggtttgcat gcttcttccg ggtcggtgta caccggcagg
     3901 tcttcgtatt cgacctcgag caatttgacc gcggcttgtg cggtgtcttc atcctctgca
     3961 gcgactgcag ccacgggctc gccgatgaaa cgaactctac cctgcgccaa aaacggtttg
     4021 tctttgacgg actctccgtg tgtccagggt acgtcttcac cagtcacaac ggccacgacg
     4081 ccgggaagct ttttcgcctt ttcgctgttg atgcgtttga tccgagcatg cgctctggtg
     4141 ctgaacagcg tttttccgta gagcatgttg ggtagaacga ggtcgtagcc gtaaaccgca
     4201 ctccc
//
LOCUS       DASULZ010000113         2441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1027373, whole genome shotgun sequence.
ACCESSION   DASULZ010000113 DASULZ010000000
VERSION     DASULZ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027373"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VFY96_02285"
     CDS             <1..837
                     /locus_tag="VFY96_02285"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX5605310.1"
                     /translation="AIRKLLAFLPSNFESMPPRKSTDDDPARSVEALEKIVPEDMKRA
                     YNIVQVIKTLVDEAEFFELKAKFARNLVIGFGRLDGYPVGFVANQPMFLAGSLDVDAA
                     DKAARFIRFCDAFNIPIVTLMDVPGFFPGKQQEEKGIIRHGAKMLYAYAEATVPKITL
                     VLRKGYGGAKQALCTREMGADQLFVWPGVELAVMGGGGAVNVLFRREIEQSADPAKTR
                     AEKIAEYQERFNGPFEALSKQFAHAALRPRETRSRLIRSLELLREKKEERPKKKHGLM
                     PV"
     gene            complement(875..>2441)
                     /locus_tag="VFY96_02290"
     CDS             complement(875..>2441)
                     /locus_tag="VFY96_02290"
                     /inference="COORDINATES: protein motif:HMM:NF011068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HEX5605311.1"
                     /translation="QNVEIFESVAPWNHVRLVGEDVVATELLLPRAHRLRPYDLGALL
                     AAGHTKVRVKVRPRVAIIPTGDELIEPGEEPKPGAVIDFNSTVLGSFVHEWGGTPIKY
                     SRVRDDMAALIEAVRRAAANSDLVTIIAGSSAGEHDLTAEVVAEAGELLAHGIDVMPG
                     KPAVLGKVDGKPFLGVPGYPVSAIVIAREILRPALEKFLGASAPGYPKVRAVLPKKIA
                     SHLGLEEYIRVTLGRVDEKLVAVPLARGAGVITTMVHADGLMRIPSLVEGLNAGEQIE
                     VELLRPLDDIENTILCTGSHDLAIGVLEDQLKQRHPEIKIAAANVGSLGGLLALQRRE
                     SHIAGTHLLEAESGVYNIPDIKRVIPNVPVVLVHLAQREQGIVVPRGNPKGISSLTDL
                     VRNDIRFVNRQPGSGTRVLLDYELKKRNMNPTEITGYDREEFTHMAVGVAIASGLADA
                     GLAVRAAARALALDFIPIANEQYDLLMGRDFFDSSRGQLLLTVISGESFKRAVNALGG
                     YDTEKAGTVLYRQ"
BASE COUNT          519 a          719 c          673 g          530 t
ORIGIN      
        1 gcgatcagaa aattgctcgc ttttctgccg tccaatttcg aatcgatgcc gccccgtaag
       61 tccaccgacg acgatccggc acgctcggtg gaagctcttg aaaagattgt ccccgaggac
      121 atgaagcgcg cttataacat cgtgcaggtg atcaagacgt tggtggatga agcagagttt
      181 ttcgagctca aggcgaagtt tgcccgcaat ctcgtcatcg ggtttggacg cctcgacggt
      241 tatcctgttg gcttcgtcgc caatcagccc atgtttctcg ccggcagttt ggacgtcgat
      301 gccgcagaca aagcggcgcg atttatccgc ttctgcgacg cgttcaatat tccgatcgtc
      361 actttgatgg atgtgccggg attttttccg ggaaaacaac aggaagaaaa aggcatcatt
      421 cgacatggag cgaaaatgct ttacgcttac gccgaagcca cggtgccgaa aatcaccctt
      481 gtcctgagaa aaggctacgg cggcgcgaag caggcgctct gtaccaggga aatgggcgcc
      541 gatcagctgt ttgtttggcc cggcgtcgag ctcgcggtca tgggcggcgg cggcgccgtg
      601 aatgtgctgt ttcggcgcga gatcgagcag tccgcggatc cggctaaaac ccgcgcggaa
      661 aaaattgccg aatatcagga aaggttcaac ggtccgttcg aagcgttatc gaagcagttt
      721 gcccatgccg cgctgcggcc gcgggaaact cgctcgcgtc tcattcgctc cctcgagctc
      781 ttgcgcgaga agaaggaaga gcggccgaag aaaaagcatg ggctgatgcc ggtgtaaact
      841 ctgatcttcg acgcaaattt tgtttcagag cttcttactg gcggtagagc accgtgccgg
      901 ctttctcggt atcgtaccca cccagcgcat tcacggcgcg cttaaagctt tcgcccgaga
      961 tcaccgtaag tagcagttgg ccacgcgacg aatcaaaaaa atcacgcccc atcagcaagt
     1021 cgtactgctc atttgcgatc gggatgaaat ccagtgcgag cgcgcgagcc gcggcgcgca
     1081 ccgcaagacc cgcatcggca agaccgctgg cgatcgcgac accgaccgcc atatgcgtaa
     1141 attcctcgcg gtcgtagccg gttatttctg tcgggttcat attccgtttt ttgagctcat
     1201 aatcgagcag gacacgggtt cccgaacccg gttgtcgatt cacgaaccga atatcgtttc
     1261 gcaccagatc cgtcagactc gagatgcctt tgggattacc tcgcggaact acgattcctt
     1321 gttcccgctg agccagatgg accagtacga cgggaacgtt tgggatcacc cgcttgatat
     1381 cgggaatgtt gtaaacgccg ctttcggcct cgagaagatg cgtgcctgct atgtggctct
     1441 cacggcgttg caacgccagc aatccgccga gacttcccac gttagccgca gcaatcttga
     1501 tttccggatg acgttgcttc agttgatctt ccaagacgcc gatcgccagg tcatgactcc
     1561 cggtgcaaag aatcgtgttc tcgatatcgt caagcggcct aagtagttcg acttcaatct
     1621 gttcgccggc attcagtcct tcgaccagac tgggaatgcg catcaaacca tccgcatgaa
     1681 ccattgtggt gatcacacct gcgccgcgcg ccagcggcac tgccaccagt ttttcatcga
     1741 cgcgtcccaa ggtgacgcgg atatattctt ccaagccgag atgcgacgcg atttttttcg
     1801 gcagaacagc acgaaccttc gggtaaccgg gcgcgctcgc tcctaaaaat ttttccagcg
     1861 cgggtcggag aatttcgcgg gcaatgacga tcgcggaaac cggatatccc ggcacgccga
     1921 ggaacggctt tccgtccact ttccccagaa ccgccggctt tcccggcatg acgtcgattc
     1981 catgggctaa caattcgccg gcttcggcca caacttcggc ggtcaagtcg tgttcgcccg
     2041 cggatgatcc cgcgatgatc gtgacaagat cagagtttgc tgccgcccgg cgcaccgctt
     2101 cgatcagagc ggccatgtca tctcgaaccc gcgaatattt gatcggcgtg ccgccccatt
     2161 catgcacgaa gctccccaaa acagtcgagt tgaaatcgat caccgctccc ggtttgggtt
     2221 cttcgccggg ctcgatcaat tcgtctccgg tcgggatgat cgcgactcgc gggcgaactt
     2281 tgacccggac cttcgtatgg cccgcggcga gcaatgcacc taaatcatac gggcgcagac
     2341 ggtgggcgcg cggcaacagc agctccgtcg caaccacgtc ctcgccgact agccgtacgt
     2401 ggttccacgg cgctaccgat tcaaaaattt cgacgttttg a
//
LOCUS       DASULZ010000114         2849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_114703, whole genome shotgun sequence.
ACCESSION   DASULZ010000114 DASULZ010000000
VERSION     DASULZ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114703"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VFY96_02295"
     CDS             <1..679
                     /locus_tag="VFY96_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated putative
                     ABC transporter substrate-binding protein"
                     /protein_id="HEX5605312.1"
                     /translation="FENRIAELIAREQNAALGYEWWAQRRGFVRNTLREGRCDLIVGV
                     PVGYDPVLTTRPYYRSSYVFVSRSDRQLRIESFDDPRLRHLKIGVQLMGDDGANAPPA
                     HALARRGIIRNVVGYTIYGDYSEPNPPARLIDAVASGAIDLAIAWGPLAGFFASREPT
                     ALTITPLATPIDASGLRFTFAIAMGVRKGDTALRDELNVMLDKRRAEIKKILDDYGVP
                     QVTETNE"
     gene            936..1424
                     /locus_tag="VFY96_02300"
     CDS             936..1424
                     /locus_tag="VFY96_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5605313.1"
                     /translation="MAPSSGRPAPVRQTELQPGAPGTPPSAEVSSVTLRSKQGEGPFD
                     KNAWAVSEGKRLFTWYNCVGCHANGGGGMGPPLMDDQWIYGSAPEQIHATILEGHPNG
                     MPSFGGKIPDQQIWQIVAYVRSLSGQLRKDVRSTRNDHMSVHPSDQARRPEQPKSSGE
                     AK"
     gene            1498..2442
                     /gene="coxB"
                     /locus_tag="VFY96_02305"
     CDS             1498..2442
                     /gene="coxB"
                     /locus_tag="VFY96_02305"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX5605314.1"
                     /translation="MLAPAGPQAGHIAALWWFMLWVSVAILTLVTVALIIALFRSTRT
                     SDSVPMTISEPEPDRRTGIIVGICVAATCIILIVFLTASVVTGHRMAQLASDQGVTIK
                     LTGYRWWWKVEYSDPEPAKTVITANEIHVPIGRPVLINTYSQDVIHSFWIPALQGKRD
                     LIPGNPSAIWLQADQPGVFEGQCAEFCGFQHAHMRLVLIAESEEQFQAWLEAQRQPSR
                     APGNSMAQRGEEIFVSGPCALCHSIRGTDANGQVAPDLTHLAGRLKIAAGTLPNTPGH
                     LAGWIVDSQHIKPGNAMPPTSLSADDLQALLAYLRGLA"
     gene            2439..>2849
                     /locus_tag="VFY96_02310"
     CDS             2439..>2849
                     /locus_tag="VFY96_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX5605315.1"
                     /translation="MNAQQPAGPRDIELSPEELHAALDQAWASPRGFFGWFANVYHKP
                     ISTRIIVTAFIFFALAGTLALIMRLQLALPENTLVGPDLYNQLFTVHGSTMMFLFAVP
                     VMQAMGLYFVPLMIGTRNLAFPRLNTFGFYMFVIG"
BASE COUNT          574 a          871 c          784 g          620 t
ORIGIN      
        1 ctttgaaaat cgtattgccg aactcatcgc gcgcgaacaa aacgccgcgc taggttacga
       61 atggtgggcg cagcgccgcg ggtttgtccg caatacgctc cgcgaaggtc gctgcgatct
      121 gattgtcgga gtccccgtag gttatgaccc ggtgctgacg acgcggccct attaccgctc
      181 gagttatgtg ttcgtctcgc gctcagaccg gcaactacgg atcgagtcct tcgatgatcc
      241 ccgcttacgt cacttgaaga ttggcgtgca acttatgggc gacgacggcg ccaatgcgcc
      301 gcccgcccac gctttggcgc gtcgcggcat tattcggaat gtcgtcggtt atacgatcta
      361 cggcgattac tcggaaccca acccaccggc acgcctaatc gatgccgtcg cctctggggc
      421 catcgatctc gccatcgcat ggggaccgct tgcgggtttt ttcgcctcgc gcgaaccaac
      481 agctttgaca attacgccgc tcgcaactcc catcgatgcc tcggggctgc gcttcacatt
      541 cgcgattgcg atgggcgtgc gcaaaggcga cacggcactt cgtgacgagc tcaacgtaat
      601 gctcgacaag cggcgcgcgg aaataaaaaa aatcctcgac gattacggcg tgccacaagt
      661 cacggagacg aacgaatgag tttgcgcgca attacgctcg ctgcggatag tctttcgcgt
      721 cgtgttgctt gctgccaact ctgttttcgt tctggctcgt tcgaaaacac cctcttggag
      781 caaccacgga catgagttcc tcgcctgacg ttgctcctct acactgtggc atttcacaat
      841 tctgctctac tatcacacgc tgtctgttct ccgtcgtgtt actagcgagc tcactcgtca
      901 taggttgcga gcgcgaacag cgaaaattta gcgacgtggc cccgtcaagc ggtcggccgg
      961 cgccggtgcg gcaaactgag cttcaacccg gagcgccggg cacgccaccg tccgcagaag
     1021 tgagttccgt caccctgcgg tcgaaacagg gcgaaggccc gtttgataaa aacgcctggg
     1081 cggtgtccga aggcaagcgg cttttcacct ggtataactg cgtcggttgt cacgccaacg
     1141 gcggcggcgg catgggaccg cccttgatgg acgatcaatg gatttatggc agcgctcccg
     1201 agcagattca cgcaacgatc ctcgagggcc atccaaacgg catgccgtcc ttcggcggaa
     1261 aaatcccaga tcaacagatc tggcagatcg tcgcctatgt gcgctcgctc agcggtcagc
     1321 tgcgcaaaga cgtgcgctcc acacgcaatg atcacatgtc ggtgcatcca tcggatcagg
     1381 caagacgacc ggaacagccg aaatcatcgg gagaagctaa atgagaattt ttacgtcgcg
     1441 gccggtgcga agcgtccggt tcgaaaatcg cgggatcgct ctaagatgat ccggtccatg
     1501 cttgcccccg caggtcccca ggccggacac atcgccgcgc tctggtggtt tatgctctgg
     1561 gtctcggtgg cgatcctcac cctcgtgact gttgcgctga tcatcgctct gttccgttcc
     1621 acccgcacca gtgactccgt tcccatgaca atttccgaac ccgagccaga ccgacgtacg
     1681 ggaatcatcg tcggtatttg cgtagcggca acctgcatta ttttgattgt ttttttgacc
     1741 gcgagtgttg tcacgggcca tcgaatggca caactcgcat ccgatcaggg tgtcaccatt
     1801 aagctcaccg gctatcgctg gtggtggaaa gtcgagtatt cagatccgga gcctgccaag
     1861 acggtcatca cggccaacga aattcacgta cccatcggcc ggccagtgtt gatcaacacc
     1921 tattcccaag atgtcattca tagcttttgg ataccggcgc tccaaggcaa gcgagatctc
     1981 ataccgggaa atcccagcgc catttggctg caagcggacc agccgggagt cttcgaaggg
     2041 cagtgcgccg agttctgcgg ttttcaacac gcgcatatgc gcctcgtgct gattgccgaa
     2101 tcggaggagc aatttcaggc atggctcgaa gcacagcgcc aaccttctcg cgcgcccggg
     2161 aactccatgg cgcagcgcgg cgaggaaatt tttgtgagcg gcccgtgcgc tctctgccat
     2221 tcgatccgcg gcacagatgc caacggtcaa gtcgcacctg atctcaccca tttggccggc
     2281 cggctaaaaa tcgccgccgg cactttgccg aacacgccgg gccatctggc cggttggatc
     2341 gtcgactctc agcacatcaa gcccggcaac gccatgccgc cgacgagtct ctcggccgac
     2401 gacctccaag cgctcctggc atatctaaga gggctagcat gaacgcacaa cagccggcgg
     2461 gacctagaga catcgaactc agtccagagg agcttcacgc cgcgctggat caagcctggg
     2521 cttcgccgcg tggttttttt ggctggtttg ccaacgttta ccacaagccg atctcgactc
     2581 ggatcatcgt cacggcattt atcttttttg cgctcgccgg caccctcgcg ctgattatgc
     2641 ggctgcagct cgcgctgcca gaaaacactc tggtcggacc ggatctttac aatcagcttt
     2701 tcacggtaca cggcagcacg atgatgtttc tgttcgccgt accggtcatg caggccatgg
     2761 gactttattt cgtgccgttg atgattggca cacgaaatct cgcgtttccc cggcttaata
     2821 ccttcggatt ttatatgttc gtgatcggc
//
LOCUS       DASULZ010000115         2610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_926430, whole genome shotgun sequence.
ACCESSION   DASULZ010000115 DASULZ010000000
VERSION     DASULZ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926430"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2159)
                     /locus_tag="VFY96_02315"
     CDS             complement(<1..2159)
                     /locus_tag="VFY96_02315"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5605316.1"
                     /translation="MALKNLNKAVPRIEGAEKVSGRMRYAADIPVPAALSAKILRTSL
                     PHARILSIDTSKARKLPGVRAIITGADVAGVMVGLRMKDMPLLAQERVRYVGEPIAAV
                     AADNEEIAEEALGLIDVQYEELPFVTDPVEAIRPGAPLLHDNPAQYKNAPERETELPN
                     VQSYGKWSNGDLEAGFQKAARIFEHTFRTPLGFHGYIEPQACTVRINDDGRIEIWASN
                     KAPFTLRARLARDLGLDEANIKVHILPVGGDFGGKTSVVEAPVCYFLAKKTGKPVRMV
                     LDYGEELTAVSHRHPAVITLRSGVDADGSLCAMQVRAVFSGGGYAALKANAEVTVQGP
                     RRAASYYRIPAIQVETICAYTNQVPCTQTRTPGSPQTTFAVESHVDIIARELGMDPVT
                     FRMKNLLNDGDPTPFGQKLKGVLVKEVLQKALDTAGWKKSKEKNIGRGVAVYERPSGA
                     GKSGAAITIEEDGAVSVNLGVPDVGPGIHTVVQQIVSEVLDLPRERVKIVVADTDNSP
                     YDSGTGGSKSTNSVGTAAYQAVNELKERIIALAATKLGCQPEDIQATNGRYSTPGRKP
                     LPFSDLLKLALDQNGGPLTHLSIYEPSRAPITSFAAQVAEVEIDPGTGRIKVKKLTTV
                     HDSGTVLNHLSYTGQIDGGVITGLGFALMEDNSLVEGRMLTANLGEFKMATIADVPKL
                     TTVLIEDASGPIPYHGKAIAEIPNVPTAAAIANAIEDA"
     gene            complement(2245..>2610)
                     /locus_tag="VFY96_02320"
     CDS             complement(2245..>2610)
                     /locus_tag="VFY96_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5605317.1"
                     /translation="HSKVFEVFPKLKIVICHCGGGLNRFIPTDHHVGQRDLKNNLYYD
                     ACCYDVLYLEAAIKQRGVDQMLFGSESPGSGGAVRPETGKPSDDLVPVIDGLGFLSAE
                     DKMKIFQKNPLKVFTKVKL"
BASE COUNT          496 a          733 c          766 g          615 t
ORIGIN      
        1 gcgtcttcga tcgcattggc aatggcggcg gccgtgggca cattggggat ctccgcgatc
       61 gctttgccat gatacgggat cggaccgctg gcgtcctcga tcaacacggt cgtgagcttc
      121 ggcacatcgg cgatggtggc cattttgaac tcgccaaggt tggcggtgag catcctgccc
      181 tcgaccaatg aattgtcttc catcagagca aatcccaacc cggtaatcac gccgccgtcg
      241 atttgtccgg tgtagctcag atggttcaac acagtgcccg aatcgtgaac tgtagttaac
      301 tttttgacct tgatacgacc cgtgccggga tcgatctcga cttcagcgac ctgagcggca
      361 aaggaagtga tcggcgcgcg cgacggctcg tagatgctca gatgcgtgag cgggccgcca
      421 ttttgatcga gcgcgagttt caagagatcg ctgaacggca acggctttcg tcccggcgtg
      481 ctgtagcgtc catttgtcgc ctgaatgtct tctggttggc agccgagctt cgtagccgcg
      541 agagcgatta ttctttcttt gagctcgttg accgcttggt aagctgctgt gccgacgctg
      601 ttcgtggatt tgctgccgcc ggtcccggaa tcatagggcg agttgtcagt atcggcgacc
      661 acgatcttca cgcgctcacg cggcagatcg agcacctcac taacgatctg ctggaccacc
      721 gtgtgaatgc cgggaccgac gtcaggcact cccaaattga cgctgaccgc accatcttcc
      781 tcgatggtaa tcgccgcgcc cgatttcccg gcgccggagg gccgttcgta aaccgcgaca
      841 ccgcggccga tgtttttttc tttggatttt ttccagcctg cggtgtcgag tgctttctgg
      901 aggacctcct tgacgagcac gcctttaagc ttttgtccaa atggcgttgg gtcgccatca
      961 tttagcaggt tcttcatgcg aaacgtgacc ggatccatgc ccaactcgcg ggcgatgatg
     1021 tcgacgtggg attcgacggc aaaggttgtt tgcgggcttc ccggcgtgcg cgtctgtgtg
     1081 cagggaacct ggttggtgta agcgcaaatg gtttcgacct gaatcgccgg gatccggtaa
     1141 tagctcgcgg cgcgccgcgg tccctggact gttacttcgg cattcgcctt cagggcggcg
     1201 tagccaccgc cgctgaaaac tgctcgcacc tgcatggcac agaggctgcc gtcagcgtcg
     1261 acgccgctgc gcagcgtgat caccgcggga tgacgatgcg atacggcggt cagctcttca
     1321 ccgtaatcga gtaccatgcg cacaggcttg ccggtctttt tcgcgaggaa atagcagacc
     1381 ggtgcttcga cgacggaagt tttgccgccg aaatcgccgc cgacgggaag aatgtgtact
     1441 ttgatattgg cctcatcgag cccgagatcg cgggcgagcc gggcgcgcaa ggtgaacgga
     1501 gctttgttcg aagcccaaat ctcgattcga ccatcgtcgt tgattcggac agtgcaggct
     1561 tggggttcga tatagccatg aaaccccaag ggtgttcgga aggtgtgctc gaaaattcgc
     1621 gctgcttttt ggaaaccggc ttcgagatcg ccgttggacc atttgccgta ggactgcacg
     1681 ttcggcaact cggtttcgcg ctcaggcgcg tttttgtatt gtgccggatt gtcgtgcagg
     1741 agcggcgctc cgggacgaat cgcctccacc ggatcggtga caaacggcag ctcttcatat
     1801 tgcacatcga tgaggccgag agcttcttcg gcgatctcct cgttgtcggc cgccacggcc
     1861 gctatcggtt cgccgacata acgcacccgc tcttgggcca acagcggcat gtccttcatg
     1921 cgcaaaccga ccatcacgcc ggcgacgtcg gcgccggtga tgatggcgcg cacgccggga
     1981 agtttcctgg ccttgcttgt atcgatgctg agaatgcgcg catgaggcag cgaggtgcgg
     2041 agaatttttg ccgataacgc cgcaggaact ggaatatccg ccgcataacg cattctgcct
     2101 gagactttct ctgctccttc gattctgggt actgctttgt tgaggttttt taacgccatc
     2161 tacttctccg tcgatctccg aggataaagt atagaggctc gaggatggtg gaaaccgatc
     2221 ttcgatcctc aatcctccag cttgctacag ctttactttc gtgaacactt tcagcggatt
     2281 tttttggaat attttcattt tgtcttccgc gctcaaaaaa ccgagcccgt cgatcacagg
     2341 aactagatcg tcggacggtt tgccggtttc cggccgcacg gcgccaccgg agccggggga
     2401 ttccgaaccg aatagcatct gatccacacc gcgctgcttg atggcggctt ccaaatagag
     2461 aacgtcgtag cagcaggcgt cataatataa attgtttttc agatcgcgct ggccgacgtg
     2521 atgatcggtg ggaatgaaac gattcaagcc cccgccgcag tggcagatga caatcttgag
     2581 cttgggaaag acctcgaaga ctttgctgtg
//
LOCUS       DASULZ010000116         5142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_926743, whole genome shotgun sequence.
ACCESSION   DASULZ010000116 DASULZ010000000
VERSION     DASULZ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926743"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1009..1158)
                     /locus_tag="VFY96_02325"
     CDS             complement(1009..1158)
                     /locus_tag="VFY96_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605318.1"
                     /translation="MRLLLSTLFRKLPVRTTKQRATICKRCGVRIYSLSYLKAHLESH
                     DRKAH"
     gene            1500..1637
                     /locus_tag="VFY96_02330"
     CDS             1500..1637
                     /locus_tag="VFY96_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605319.1"
                     /translation="MSRFVDYMLFNYFEPALGWVLSHPIISSLGVLALIYFSVRNYRM
                     L"
     gene            1851..2717
                     /locus_tag="VFY96_02335"
     CDS             1851..2717
                     /locus_tag="VFY96_02335"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605320.1"
                     /translation="MPLVSAVAASHAPNILLEPGSEWEDFMDLHYSMAPKGSATRPTL
                     EQQRKLRKDAEEAFAVLRKDLQRSHPDVLVVVANDQFVNFFWNNIPTFFVTIGEEVKG
                     QFTRHRFHYRNHKELGRAIIRAGMEMGIDFSYGEKIELQHTQNVPLYFLLREPSIPIL
                     PIYVNTWVDPAPSPRRCYQVGELIRAVAEQSKERIAILATGGLSHFPGSPRIGEIDEK
                     FDHQLLEVLREGNGKTLADYSVQELLHAGDSEFLNWMVVIGAVGDAKASYTAYMPDFV
                     ATGWGFVSWRSV"
     gene            2756..2959
                     /locus_tag="VFY96_02340"
     CDS             2756..2959
                     /locus_tag="VFY96_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605321.1"
                     /translation="MKDSVYQQALTDFEGVMANYELTAEEKTALRDGDPRKLRQLGVH
                     GMLCLYIKRLQPEFRDNIYWQQK"
     gene            3065..3856
                     /locus_tag="VFY96_02345"
     CDS             3065..3856
                     /locus_tag="VFY96_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5605322.1"
                     /translation="MDLGLTGKVALVAGASQGMGRASALGFAREGAKVAICARGETAL
                     NDAAAMIRKQTGGEVFALVADMSKSEDIKKFVNQSVEHFGRLDIIVNNAGGPPPGEFM
                     KFTDEDWQNAYNLSFMSTMRMTRAAVPHMRKVGGGRVINITSYSVKEPITGLVLSNAV
                     RSAVIGLAKTLSRELARDNILINNICPGRIDTERAQKLNKARADRLNRPVEEINKEMA
                     AEVPLGRYGTAEETADVIVFLGSERASYVTGTTIQIDGGLVRGIQ"
     gene            3856..4179
                     /locus_tag="VFY96_02350"
     CDS             3856..4179
                     /locus_tag="VFY96_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605323.1"
                     /translation="MVHFVIQWDLPDQKANLTIYSNKARNDWLPTTLTHTGVIEIRNY
                     RNPLEVTPHVAITIDFETLDNWRDFIDSQDYIRIMRELRILGCNNFTVNVWAPSKYFP
                     ESMRP"
     gene            complement(4257..>5142)
                     /locus_tag="VFY96_02355"
     CDS             complement(4257..>5142)
                     /locus_tag="VFY96_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605324.1"
                     /translation="APTEPKPIGHFATGSTGTHRNFYDGGNFVHAAAGAPTLNGKIYR
                     IVNIADPDKPREIGRFSLPEQEQGALTDGLKFSCHGPAHVEGDRAYLSYGDGGGIVLD
                     VSDMSAPKMIGQLAFRGITSSQGIHTFLPLPRRKIALINDEAIRENGDENLNLAGIVD
                     ISDERRPRMLSLFPLPEPPAESGVKNFYAKGGRFGPHNHHHANHQACLENRDDIAYLT
                     YFNAGLRVYDIRDARQPKEIGYFVPGDPVTRIGLKPSRLVAQSEDVLVDHRGFIYLSD
                     KNHGVHILRLKQVWPYAT"
BASE COUNT         1222 a         1340 c         1388 g         1192 t
ORIGIN      
        1 agtcttatta tttaaaatgt caaatttttc tagcgtcatc ccaagaactg cgggaattca
       61 ggcgactcga aatccaacaa ctaaatgtgt cgaagaattt tgtcacagcc ggaaaacttg
      121 cctcaactct ccggcttcca acatctcaac ggaaccgtca gtgatatcag tcttccatcg
      181 gcatgggcgt acctctttaa aaatcgcgac ccgtcttggt acgggtcgtt gccgtctatt
      241 tgaacccact cgaccgcgcg ccaggtcgca atttgatgag agatcatccc actcacaaga
      301 tgagctattc acatccgcgc tcgagtgtac cttcatgcac aaacccggga aatttcttcg
      361 aaggccttaa aagaatccac ctcaggcagg gtaacgtgaa tggcgactgt catttatcaa
      421 actgtcattt atcaaatttc ctgaagcgcg agtggagcaa aagatctttg cttaccgggt
      481 aatcagtgac agcatggatt caaacacgcg tgagatcgcc gcacttgatc gagctttggc
      541 aagacatgaa cggttgacaa atttcgcgac cgagagaatc agtggtcagt aacccgcctg
      601 tgaaaacaag ttttcaaaaa cgagtccaac gatttacgat acggtctctg gaagtctgat
      661 gggcttggag ctactcaccg ggctggtggt gcatttggtc ccggcgctat tggttggcct
      721 tgccgttatc aacttgccac gctgaagtgc ggcgagagtc atccggatcg cacgatattg
      781 tagagtcgcg ccacatacgg aacgccttcg ttccgcggtc gaaagaataa tttaacggcc
      841 tgcattgctg ctcctggtca gaagaagcca gcgcgaatga gttgtatgaa acgaatcaat
      901 cattcctcat gacgggtgac gtaagactca atctggtgct cttgttcgca taggacagcg
      961 ataattttgc ctcatggaaa gcggaaaatt ccattgagtc tccgatgact aatgggcttt
     1021 acggtcgtgc gattccaggt gggccttgag gtaactcaaa gaatagatac gcacgccgca
     1081 gcgcttacaa atggtcgcac gctgtttggt tgttcgcacc ggcagttttc tgaacagggt
     1141 cgatagcagt agtctcatcg tttgctccct ctgtaaattt gatgggtcgt cgatagagca
     1201 acgccgatgc caccagtttg agtaattcgt ggaccgcaaa agtctctagc ccatgcgaag
     1261 ttgggctcgg ctgtcacctg ggaataagcg cttttgttcg agtacttctg tccaaagcta
     1321 ggctactttg agcaaacttg tacagacaat ggctcaattg ttacctggat tgccaaagcg
     1381 ccggtccatg gcctctccta tgcctccgtc cgggcaaata ctcaaggagg caaaaatgat
     1441 ctgtccccag caggcgagtt ttgttataac tatcgatcaa aatcgggtga ggattgggaa
     1501 tgagccggtt tgtcgattat atgttgttta actacttcga gcccgcactc ggttgggtgt
     1561 tgagccatcc gattatttcc agtcttggag tacttgccct tatctatttc tcggtccgta
     1621 actatcgcat gctctgaccg atcgattctc tttctaccct ctatttcttg cattcaatag
     1681 cgcgcatccg cgatactctc gtaccgcatg ggttgagcat tccgtccgca tccgctctga
     1741 gttagcgccg aaatcgactc aaaattccta gcctaaccga ctgactggct cggttgcttt
     1801 ttctcaggtc ggggactagg attttttcaa aattcatcga ggagtcggcg atgcctctgg
     1861 tttccgccgt ggccgccagc cacgcaccca atatcctgct cgagccggga agcgaatggg
     1921 aagatttcat ggatcttcac tacagtatgg cgccgaaggg ctccgcgacg cggcccacac
     1981 tcgagcagca aagaaagctt cggaaggatg ctgaagaggc tttcgcggtc ctgcgcaagg
     2041 acttgcaacg aagtcacccc gacgtcctgg tcgttgtcgc caacgatcag ttcgtgaatt
     2101 ttttttggaa taatattccg acttttttcg tgaccatcgg cgaagaagtc aaaggccagt
     2161 tcacgcgcca ccggtttcat tatcgaaatc ataaagaact cggcagggcg attatccgcg
     2221 ctgggatgga gatgggaatt gatttttcct atggcgagaa gattgagctc cagcatacgc
     2281 aaaatgtgcc gctttatttt ctccttcgag agccgagcat tcccatcttg ccgatttacg
     2341 tgaatacctg ggtcgatccc gcaccgtcgc cgcgccgttg ctatcaagtg ggcgaattga
     2401 tccgcgcggt ggcagaacag tcgaaagagc gcatcgcaat cctcgcaact ggcggcttat
     2461 cgcattttcc tggctcgcca agaattggcg aaatcgacga aaaattcgat caccagctgc
     2521 tcgaggtttt gcgcgaagga aacggcaaga cactcgcgga ctattcggtt caggagcttt
     2581 tacacgccgg cgatagcgag tttctcaatt ggatggtcgt gatcggcgcg gtcggggatg
     2641 ccaaagcatc gtacactgca tacatgcccg actttgtcgc caccggttgg ggctttgtca
     2701 gctggaggtc agtgtgagtt gttacgattt gagccggtta ttattcgatc tcaaaatgaa
     2761 agactcagtg taccaacagg cgctgaccga cttcgaaggc gtcatggcga attacgagct
     2821 cacggccgag gaaaaaaccg cgcttcggga cggcgatccg cgcaagctgc gccaactcgg
     2881 cgtgcatggg atgctttgcc tttacatcaa gcggctccag ccggaattcc gcgacaatat
     2941 ctactggcag caaaaataaa ttaaagatcc agacaacagc gactgtttcg accaagcaaa
     3001 tcttttgacc gtccactagg cgtttgctag tgtatcggcg aatcaagcag ggggaaaggc
     3061 cagcatggat ttaggactga cggggaaggt cgcgttggtg gcgggagcga gccagggaat
     3121 gggacgcgcg agcgcgctcg gcttcgcccg cgaaggagcg aaggtcgcca tctgcgcgcg
     3181 cggcgagacc gcgctgaacg atgcggctgc gatgattcgt aaacaaaccg gcggcgaagt
     3241 gttcgcccta gtggcggaca tgtcaaaatc ggaagatata aaaaaattcg tcaaccaaag
     3301 cgtcgagcac ttcgggcgtc tcgacatcat cgtcaataac gccggcggac cgccgcccgg
     3361 tgaattcatg aaatttaccg atgaagattg gcaaaacgcc tacaacctga gcttcatgag
     3421 caccatgcgc atgacgcgtg cagcggtgcc gcacatgcgc aaggtaggtg gcggcagagt
     3481 cattaacatt acttcttatt cggtcaaaga accgatcacc ggactggtgc tgtccaacgc
     3541 cgtgcgcagc gcagtgatcg gcttggcgaa aacgctctcg cgcgagctag cccgagacaa
     3601 catcctgatc aacaatatct gccccgggcg aatcgatacc gagcgcgcgc aaaaactgaa
     3661 caaagcgagg gcggaccggc ttaaccggcc ggtggaagag atcaacaagg aaatggcagc
     3721 ggaagtgccc ctcggccgtt acggcaccgc ggaggaaacg gcggacgtca ttgtctttct
     3781 cggttcggag cgggcgagct atgtcaccgg caccacgatt caaatcgatg gaggcttggt
     3841 aagggggata cagtaatggt ccatttcgtc attcaatggg acctgccgga tcagaaagcc
     3901 aacttgacga tctattctaa caaggcgaga aatgactggt tgccgactac gctgactcac
     3961 acaggggtga ttgaaatccg caattaccga aatcctctcg aggtcacacc gcacgtcgcc
     4021 atcactatcg atttcgagac gttggacaat tggcgcgatt ttatcgattc ccaagactac
     4081 attcggatca tgcgcgagct gcgcattctg ggctgtaaca acttcaccgt caacgtgtgg
     4141 gcgccgtcca aatatttccc tgaatcgatg aggccgtaga ttagaagttc tctttcaaca
     4201 atttcgagcg cagcgccacc gtcccgggtg gcgttgtcgt tttcactggt aaactgctaa
     4261 gtcgcatacg gccaaacttg tttgagacgg agaatgtgaa ccccgtgatt cttgtcggat
     4321 agataaatga acccgcggtg atccacaagc acatcttccg actgcgcgac caggcgactg
     4381 ggcttgagtc caatccgagt tacgggatca ccgggcacaa aataaccgat ctctttcggt
     4441 tgacgcgcat cacggatgtc gtagactctc agcccggcat tgaagtaggt cagataagcg
     4501 atgtcgtcac ggttttcgag acaggcttga tgattcgcat gatggtggtt gtgaggaccg
     4561 aagcggccgc ctttggcgta aaaatttttg accccgctct ccgccggagg ctccggcaac
     4621 ggaaacagcg acagcatgcg cgggcgtcgc tcgtcgctga tatccacgat cccagctagg
     4681 tttaagtttt cgtcgccgtt ttcccggatc gcttcgtcgt tgatcagggc gatcttacgg
     4741 cgcggcaagg gcaggaacgt gtgaattccc tggctggagg tgatgccacg gaaggcgagc
     4801 tggccgatca tcttcggcgc cgacatgtcg gaaacgtcga gaacaatgcc gccgccatcg
     4861 ccgtatgaga gatacgcgcg gtcaccttcc acatgagccg gcccatgaca agaaaatttc
     4921 aagccatctg ttaatgcgcc ctgttcttgc tccggcaatg aaaaccggcc gatctcgcgc
     4981 ggcttgtcag gatcagcgat gttgacgatg cgataaatct ttccgttcaa agtcggggca
     5041 cccgcggcgg catggacgaa attgccgcca tcgtaaaaat tccgatgggt tcccgtgctt
     5101 ccggtcgcga aatggccgat gggtttcggt tcagtcggtg cg
//
LOCUS       DASULZ010000117         8673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_216260, whole genome shotgun sequence.
ACCESSION   DASULZ010000117 DASULZ010000000
VERSION     DASULZ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8673
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216260"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VFY96_02360"
     CDS             <1..373
                     /locus_tag="VFY96_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HEX5605325.1"
                     /translation="GGLASTLTALQNAGGNIEFVVARRAADKPGTGVVFLSPIKGAKQ
                     KTAAERSGLATSESLHSVRVEGPDRAGLGAKMTEALAGAGINLRGISAAALGRRAVSY
                     FAFDSAADADNAMRVLKKTLK"
     gene            complement(435..1508)
                     /locus_tag="VFY96_02365"
     CDS             complement(435..1508)
                     /locus_tag="VFY96_02365"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5605326.1"
                     /translation="MAMVFFMLIAALVYEPHSGHAATADEIIKKTSSLSGSQRRSFLE
                     EGAKKEGEIVFYTSLSLTDYPKIMPHFERAYPFIKTNTYRATPSGVFNKADTEARAGR
                     FAVDVIGSAPVEMWQLKQRKLSAPYLSPEQKSLPSGSHDPEGYWQGFEVTPLVLAFNT
                     KQVNGTDVPRSYQDLLNPKWKGKMSLGTEEYTWFNILLESMGAKKGAEFMQALAKQDL
                     QMPGSSSVMRVQLMLAGESAIAIAARGRRVTEYKQQGAPIDFRILDPYAGEPNFVALM
                     QRAPHPHSALLFIDWILSEEGQTRLADAAGRIPVRKGIKQKPWVQELFQKDFVFLSPS
                     SIGPNLNSLIEQYNQIFGIRKTK"
     gene            complement(1524..1901)
                     /locus_tag="VFY96_02370"
     CDS             complement(1524..1901)
                     /locus_tag="VFY96_02370"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5605327.1"
                     /translation="MAKLRHLAILTHQPDKLATFYKRVFDMTEMYRTKNGSVHLSDGE
                     VNLAILNATDPKEPGHQPGLYHFGFQVEDNREVAKRIQEIYPEGAPKEREATYAEGRG
                     SDPDGNLFDISTVGWGQKRRMDL"
     gene            complement(2004..2924)
                     /locus_tag="VFY96_02375"
     CDS             complement(2004..2924)
                     /locus_tag="VFY96_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5605328.1"
                     /translation="MRVKSMSRRDFARYALAAAFFPWKEQVAMADGISTKPIPKTGER
                     LAAIGLGTWQTFDVGAVESARAPLREVLHEFVRTGGTVIDSSPMYGKSESVTGELATE
                     LDVQKKIFFATKVWTSGRDAGIRQIEESYRRLRVDRMDLLQVHNLVDYRTQLSTLRAW
                     KEQGKVRYIGVTHYAASAYDELARVLAREDLDFVQLNYSFAERDAEKRLLPLAAEKRL
                     AVLINRPLAAGNLFSRVRGKPLPAWANELGCATWAQFFLKFVISHPAVTCAIPATRKV
                     QHLTDNMRAAFGPLPDASARERMARYFAGL"
     gene            complement(3003..3869)
                     /locus_tag="VFY96_02380"
     CDS             complement(3003..3869)
                     /locus_tag="VFY96_02380"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX5605329.1"
                     /translation="MTLSLIVWLLSFFLVAPARALDLPPLTSPVTDMAGMFPPASLSD
                     LTQRLGRFETESGTRVFVITVRSLEGEKITHLAERAFSALPLSEAERRKTVLLAVART
                     ERLVALRAGSQLRELLPEPQTHEKLQAQVALYWDGMRPDLGIHGAVYYLVRVLRGDIR
                     VGSQTEEEKLEQVSLRGGEAGAIFALCLGPFLAFFVGVFWGIYATQYGVQRGPRLLMS
                     GIFGGATAKIVGMLMSFLGNYSDDLWYFIMFLAIPLAVFGSLTEFWMSGDWSGIPRIK
                     ERPRKPEDRMGI"
     gene            complement(3866..4645)
                     /locus_tag="VFY96_02385"
     CDS             complement(3866..4645)
                     /locus_tag="VFY96_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605330.1"
                     /translation="MIEQNVEQLARLAAAYRKAEVEVVNSFFEKPRTNTDHVLWLKAQ
                     GFKEYSAIKPLIAALAALYRQIDDGIDRHRYAELTEKLADETRHARLVMDLLEEITGN
                     KITPRDLTWLPQDRKLARVRAKYSKSYAALLHGSEKISSGEIARRDEELERAAITLTE
                     GGGGALYQVCAKLKKTGIEAKIAVVFREILRDEVGHKENGARELARLIRSQTAYRRAA
                     KIICDVSQQRLRMRNEQFGFPLAASDLMALDGFARRSIEPR"
     gene            complement(4638..5180)
                     /locus_tag="VFY96_02390"
     CDS             complement(4638..5180)
                     /locus_tag="VFY96_02390"
                     /inference="COORDINATES: protein motif:HMM:NF017830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydratase"
                     /protein_id="HEX5605331.1"
                     /translation="MKELIPIDINSLIEEVKQQGQGRRVLWQDSESIAFLSSGRKERW
                     DFHIDPADEVTLQLSGVQNLVYRDEAGTEHTAKIKAGQILLCPAGVPHSPRLEPDSWF
                     IVFERKRRPGEIDHFKWFCRKCAEQVYETSVEVGDYRADPVGQVYEKFASDETLRTCK
                     RCGEVIPAPPGRGLEAMRHD"
     gene            5296..6087
                     /locus_tag="VFY96_02395"
     CDS             5296..6087
                     /locus_tag="VFY96_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX5605332.1"
                     /translation="MAERVWDRFLTEQDKATLVGRADRRIGFGERPALLLIDLYRWVF
                     GDKPELVTEAIKTWPGSCGLAGWNSLPHIKKLLGKAREVGIPIIHVTGMDGVAVEPWA
                     FRRDNKKRATMSKEELDRFRRRYDIVDEVKPIPGETVFKKVSPSAFWGTPLIGHLNFL
                     GIDTIITCGESTSGCVRASVVDGTTNRLRMIVAEECVFDRHEACHAINLFDMNQKYAD
                     VLPLDEILKYLDAWRAEKAGQVGYANNSIEYLKDLAIGEEYKGLR"
     gene            complement(6171..7337)
                     /locus_tag="VFY96_02400"
     CDS             complement(6171..7337)
                     /locus_tag="VFY96_02400"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5605333.1"
                     /translation="MKLSPLTLYVSAAVCQTAGSSGAAMLAPFFMREHGYSVATAGIP
                     LVANGVGRVCSDVMSGVMATYFSPGLLLIIAMMLGLVTSIVGYAFIDAMPVFTAAWIV
                     FGLTEAMFALSLRKIGFDQAAPERQGRVQGQMASALGIGFTLGPLLGGWVGKWLGADS
                     LFLLYAAPQFVGMLLIFYAGAHRYRRTVKPESLRVWRDGGLLLRKWPFLASCLAICQS
                     FLFLVGVTRVAFPFLAVNLRGISLETVGTLVSISRLTDTLGRYTGGWLCDRIKPGRVI
                     LLGIALSIPMFLLQVAGTNLVTLLVPLAIMTMGFGFTNVGATTFALQSAHHGAKELAL
                     GISRASTSVGQTIGPLACGALIQQLGYAGGFEAMALSSLIVLALAWYGLQKQSN"
     gene            7450..7758
                     /gene="glpE"
                     /locus_tag="VFY96_02405"
     CDS             7450..7758
                     /gene="glpE"
                     /locus_tag="VFY96_02405"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001195.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HEX5605334.1"
                     /translation="MEVPQIQIQDAKNKLDGKECLFIDIRDPGSYEEAHIPGAIRLHD
                     GNVQEFLQSANKSKEVIVYCYHGNSSLGATAFLIENGFTNVASMSGGFEAWRQVYEHE
                     "
     gene            7846..>8673
                     /locus_tag="VFY96_02410"
     CDS             7846..>8673
                     /locus_tag="VFY96_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HEX5605335.1"
                     /translation="MSVVVGSGDYKYEVIENWGTLPDGWTFHEVAAVAVDAQDQVYCF
                     TRGKHPVIVFDRDGNFLRAWGDGVFKRAHGATIGPDQTIFLTDDGDHTVRQCTLEGKI
                     LMTIGVPGKCATYQCGEPFNRCTHVAFSAKGEIYVSDGYGNSRVHKYSPDGKLLFSWG
                     ESGSDPGQFNIVHNIVTDKNGWVYVADRENNRIQVFDANGKYETQWNNMHRPCALYMD
                     TTKNDPICYVGELGPGLGVNKDAPNLGNRVDIYDKTGKRLARLGDIWPGEKPGQFIAP
                     "
BASE COUNT         2018 a         2363 c         2414 g         1878 t
ORIGIN      
        1 cggcggttta gcgagcacac tgacggccct acaaaatgcg ggcgggaaca ttgaatttgt
       61 tgtggcgcgt cgcgctgccg acaagcccgg caccggagtc gtttttctca gcccaatcaa
      121 gggcgccaaa caaaaaacgg cggctgagcg gtccggtttg gccaccagtg aaagccttca
      181 ttcggtccga gtcgaaggtc ctgaccgagc cgggctggga gctaaaatga ccgaggccct
      241 cgccggagca ggaatcaacc tgcgcggcat ttcggcggcg gctttgggcc ggcgcgcggt
      301 gtcctatttt gcattcgaca gcgccgcgga tgcggacaat gccatgcgcg tattgaagaa
      361 aacgctgaag tagggccaac cggatcctac cgggaaagcg ggtccttgct cgattcagta
      421 cgccgattgc gacgctactt agttttgcga atgccgaaga tctgattgta ttgctcaatc
      481 agcgagttca ggttaggtcc gattgaagaa gggctgagaa acacaaagtc cttttgaaag
      541 agttcttgga cccagggttt ttgtttgata cctttgcgca caggaattct gcccgccgca
      601 tcggcgaggc gtgtttgtcc ttcttcggac aaaatccaat cgataaacaa cagcgccgaa
      661 tggggatgag gcgcgcgctg catcagcgct acaaagttgg gctcgccggc gtacggatct
      721 agaatccgaa agtcgatcgg ggcgccttgc tgcttgtact ccgtcacgcg gcgcccacgc
      781 gctgctatcg caatcgccga ttcgcccgcg agcatcagct gcacccgcat aacactgctc
      841 gaaccaggca tttgcagatc ctgtttcgcg agcgcctgca taaattcggc acctttttta
      901 gcgcccatgg attccaacaa aatattaaac caggtgtact cctcggtgcc gagactcatc
      961 ttacctttcc acttcggatt caacagatcc tggtagctgc gtggcacatc ggtgccgttc
     1021 acttgcttgg tattgaaggc taagacgagc ggcgtcactt cgaatccctg ccaatatccc
     1081 tcgggatcgt gtgaaccgga cggcaatgac ttctgctcgg gcgaaagata gggcgcggaa
     1141 agcttgcgct gcttgagctg ccacatctcc accggagccg atccgataac atcaacagca
     1201 aaacggccgg ctcgcgcctc ggtatcagct ttattaaaaa cgcccgatgg tgtggcgcga
     1261 tacgtattgg ttttgatgaa aggataagct cgttcgaaat ggggcatgat tttcggatag
     1321 tcggtcaagc tgagggaggt ataaaaaact atttcgcctt cttttttggc tccttcctcg
     1381 agaaagctcc tgcgttgcga gcccgagagg ctgctggttt ttttaataat ttcatccgcg
     1441 gttgctgcat gacctgaatg cggctcataa accagcgccg cgatgagcat gaaaaatacc
     1501 atcgccatcg aagagcgccc gttctacaga tccatgcgcc gcttttgtcc ccagccgacg
     1561 gtggagatat cgaacagatt gccgtccgga tcggagccgc ggccttccgc ataggtcgcc
     1621 tcgcgctcct tgggcgcgcc ctctggatag atctcctgaa tacgcttggc gacttcccgg
     1681 ttgtcttcaa cctgaaaccc gaagtgataa agtcccggct ggtgccccgg ctctttggga
     1741 tccgtagcgt tcagtatcgc gaggttcact tcgccgtcgg aaagatgcac cgaaccgttt
     1801 ttggtgcgat acatctcggt catatcgaac acccgcttat agaaggtcgc gagcttatcc
     1861 ggctgatgag ttaggatggc gagatgtcga agcttggcca tagcgttgtc ctcctgatcg
     1921 atttcgagtt cagccgtatc atcgcgaatt tacgctgtca aattatcgtc gtcggcagga
     1981 aggcaaaact catcatctcg cattcacagt ccggcaaaat agcgcgccat gcgctcccga
     2041 gctgaagcgt caggcaacgg accgaacgct gcacgcatat tatcggtaag atgctgcact
     2101 ttcctcgtgg cgggaatcgc gcaggtaacg gcgggatgag agatcacgaa tttcaaaaag
     2161 aattgcgccc aagtcgcgca gccgagttcg ttcgcccagg cgggaagcgg cttgccgcga
     2221 actctgctga atagatttcc cgccgcgaga ggccggttga ttagcaccgc aagccgcttt
     2281 tccgctgcca aaggcaacag tcgcttttcc gcatcacgct cggcaaaaga atagttgagc
     2341 tggacaaaat ccagatcctc ccgtgcgagc acacgagcga gttcgtcgta ggcgcttgcc
     2401 gcataatgag tgacgccgat ataacgcact ttcccctgtt ctttccaagc ccgcagagtg
     2461 ctgagctggg tgcggtaatc gacaagatta tgaacctgca atagatccat cctgtcgaca
     2521 cgcaaacgcc tatacgattc ttctatttgg cgaatccctg catcgcggcc gctggtccaa
     2581 accttggttg cgaaaaaaat tttcttctgc acatcgagct cagttgcgag ctcacccgtc
     2641 accgattcgg actttccgta catcggcgat gaatcaatca ccgttccacc cgtgcgaacg
     2701 aattcatgca gcacttcgcg aagcggtgcg cgcgctgatt cgacggcgcc gacatcgaag
     2761 gtctgccagg tgccgagacc gatcgccgct agccgttcgc ccgtctttgg aatcggtttg
     2821 gtgctgatgc catccgccat agcgacttgc tccttccatg gaaaaaatgc cgcggcgaga
     2881 gcgtatctgg cgaaatcgcg ccgggacatg ctttttacgc gcataatttt gttctttaga
     2941 atagatgtgg atcttctcga cgggtgatca ggtccgagga atcggccata ttcatggtcg
     3001 gatcaaatgc ccatacggtc ttcgggtttg cgcggtcgtt ccttaatgcg cggaatgccg
     3061 ctccagtccc cggacatcca gaattccgtc aagctgccga agacagcgag gggaatggcg
     3121 agaaacatga tgaaatacca aagatcatcg ctatagttgc ccaaaaaaga catcagcatc
     3181 ccgactatct tcgccgtagc cccgccaaaa attccgctca tgagtaaacg cggaccgcgc
     3241 tgcacgccat actgggttgc atagattccc caaaaaaccc ctacgaagaa agccaggaag
     3301 ggtccgaggc aaagcgcgaa aattgcgcct gcttcaccac cgcgcaagga gacttgctcc
     3361 aatttttctt cttcggtttg actgccaacg cggatgtcgc cacgcagaac gcgaaccaga
     3421 taatagacgg caccatggat gccgagatcg ggtcgcatgc cgtcccaata aagtgcgact
     3481 tgggcctgca gtttttcgtg cgtttgcggc tcgggcagta attcacgtag ctgagagccg
     3541 gcacgcaaag cgaccaatcg ttctgtgcgc gcgacagcca gtaagacggt tttacgccgt
     3601 tctgcctcgc tcagcggaag agctgaaaac gcccgctcgg cgagatgcgt aatcttttct
     3661 ccttcgaggc ttcggacggt gataacaaaa acgcgagttc cgctctcggt ctcaaagcgg
     3721 cccagacgct gggtaagatc actcaggctt gccggtggaa acatgccggc catgtcggtg
     3781 accggtgatg tgagaggcgg caaatccaaa gcacgagcgg gagcgactaa gaaaaagctc
     3841 aacaaccaga cgataagaga aagcgtcatc gtggctcgat cgatcgccgg gcgaaaccgt
     3901 cgagtgccat caaatcgctt gccgccagcg gaaatccaaa ctgttcattg cgcatacgca
     3961 aacgctgctg cgaaacatcg cagatgattt ttgccgcccg tctataggcg gtctggctgc
     4021 ggatcaggcg tgcgagctcc cgcgcgccgt tttctttgtg cccgacttca tcacgcaaga
     4081 tttctcgaaa gaccacggct attttcgctt caatcccggt tttcttgagt ttagcgcaga
     4141 cctgatagag ggcgccgccg ccaccttcgg tcaaggtgat agcggcgcgc tcgagctctt
     4201 cgtcgcgccg tgcgatttca ccggagctga ttttttccga gccgtgcaac agcgccgcat
     4261 agctcttgga gtatttggct ctcaccctgg cgagttttcg gtcttgcggc aaccaggtga
     4321 ggtctcgggg cgtaatcttg ttcccggtaa tttcctccag aagatccatg accagacgtg
     4381 catggcgcgt ttcgtccgcc aatttttcgg tcagctcggc atagcgatgt cgatcgatac
     4441 cgtcgtcaat ttgtcgatac agagcggcca gcgcggcgat gagcggtttg attgcagagt
     4501 attccttgaa tccctgcgct ttgagccaga ggacgtgatc tgtgttggta cgtggcttct
     4561 caaagaagct gttgacgact tcaacttccg cctttcgata ggctgccgcc agtcgcgcaa
     4621 gttgctcgac attttgttca atcatgccgc attgcttcca gtcctcggcc gggtggcgca
     4681 ggaatgactt cgccgcagcg tttacaggtg cgcagcgtct cgtcactggc aaatttttca
     4741 taaacctgcc ctaccggatc ggcccggtag tcgccgactt ccaccgaggt ctcgtatact
     4801 tgttcggcgc atttcctgca gaaccatttg aagtggtcga tctcgccagg gcggcgtttg
     4861 cgctcgaaga cgatgaacca ggaatccggt tccagccgcg gcgaatgggg cacgccggcg
     4921 gggcacaaca aaatctgacc ggctttgatc tttgcggtat gctccgttcc cgcttcatcg
     4981 cggtaaacga gattttgcac tccgctcagc tgcaaggtga cctcgtcggc cggatcgatg
     5041 tgaaaatccc aacgctcttt gcgcccgctg ctcaagaagg cgatggattc cgaatcctgc
     5101 catagcacgc gccgcccttg gccttgctgt ttcacctctt cgatgaggct gttgatatcg
     5161 attggaatca gctctttcat ggaccgacta tagcgctatc gaaatgccag gggcaacagc
     5221 gctctgggac cggttctgcc ttgacccctg aataaacggg atgataagtc ttgagcatta
     5281 aaccggaggt gtttcatggc cgaacgagtg tgggatcgtt ttctgaccga gcaggataag
     5341 gcaacattgg tcggcagggc tgatcgtcgt ataggtttcg gtgaaagacc agcactacta
     5401 ctcatcgatc tttaccgctg ggtttttggc gataagcccg agctggtgac cgaagcgatc
     5461 aagacttggc cgggcagctg cggcctggcc ggctggaact ctcttcccca cattaaaaaa
     5521 ctactcggta aagcacgcga agtgggaatt cccattattc acgtcacggg gatggatggc
     5581 gtggcggtag agccgtgggc ttttcggcgc gacaataaaa aacgtgcgac catgtcaaag
     5641 gaagagctcg accgttttcg tcggcgttac gatatcgtgg atgaagtgaa accgattcca
     5701 ggagaaacgg ttttcaaaaa agtctcaccg agcgcgttct ggggcacgcc cctgatcggc
     5761 catctgaact ttctcggcat cgatacgatc attacttgcg gcgaaagcac gagcggctgt
     5821 gtgcgcgcga gtgtcgtcga tggaacgacc aaccgtttgc gcatgatcgt tgccgaagaa
     5881 tgcgtttttg accgccacga ggcctgtcat gcgatcaatc ttttcgatat gaaccagaaa
     5941 tatgccgacg tcctgccgct ggacgaaatt ctcaaatacc tcgatgcctg gcgcgcggaa
     6001 aaagctggcc aggtcgggta tgccaacaac agtatcgaat atttgaaaga tctagcgatc
     6061 ggtgaagaat acaagggcct gcgctgaggg acggacgtaa tctcaaagtc caacgttcag
     6121 cttttttcgc atcggttgtc catgaatctg aaagttaagc tttgaagcga ttagttggat
     6181 tgtttttgaa gaccgtacca ggcaagcgcc aggacgatca gcgagctcag cgccatcgct
     6241 tcgaagcctc cggcataacc taactgctga atcagcgcac cgcaggccaa cggtccgatc
     6301 gtttggccca cagaggtgga cgcgcgcgag atgcccagcg cgagttcctt ggctccgtgg
     6361 tgagcggact gcagcgcaaa agtcgtcgca ccgacgttcg taaatccgaa acccatggtc
     6421 atgattgcga gcggaaccaa cagagtcaca agattcgtgc cggcgacctg aagtaagaac
     6481 atcgggatgc tcagcgcgat gccgagcagg atgacgcggc cgggtttgat tcgatcacaa
     6541 agccaacccc cggtatagcg tcccaaggtg tccgtcaacc gggatatcga aaccagcgtg
     6601 ccgacggtct ctaaactgat gccgcgcaga ttgaccgcga gaaatggaaa cgcgacccga
     6661 gtcacaccga cgaggaaaag gaacgattgg caaatcgcta aacacgaggc taggaaaggc
     6721 catttgcgta acagcagacc accgtcacgc cagactctga gcgattcggg ctttacggtt
     6781 cgtcgatagc ggtgcgcccc ggcatagaag atcagcagca taccgacaaa ttggggcgcc
     6841 gcgtaaagca gaaataaact atcggcccca agccatttgc cgacccaacc gccgagcaag
     6901 ggtccgaggg tgaagccgat ccccagggca gaagccattt gcccctgcac tcttccttgt
     6961 cgctcgggcg cggcttgatc aaaaccgatt tttctcaaag atagggcgaa catcgcttcg
     7021 gtcaagccga acacgatcca tgcggctgta aacaccggca tcgcatcgat gaaggcatag
     7081 ccaacaatgc ttgtgaccag gccaagcatc atcgcgatga tgagcaatag gcccggactg
     7141 aaataagtag ccattacgcc tgacatcaca tccgagcaaa cccggccgac cccgttagcg
     7201 accaagggga ttccagcggt cgcgacggaa tatccatgtt ctctcatgaa gaacggcgcc
     7261 aacatcgccg ccccggacga tccggcggtc tggcaaacgg ccgccgaaac atacaaagtt
     7321 agaggggaga gcttcattcg cgcgagtata gcaactcgtt catttacagc atatcaacgg
     7381 gcaaaagcta tgactgtgga caggcaaaac cgggcttgat aaaacgacca cgtcagcagg
     7441 agtgaaacga tggaagttcc gcaaattcaa atacaggacg cgaaaaacaa gttggacggg
     7501 aaggaatgcc tgtttatcga tatacgcgac ccgggttcgt acgaagaggc gcacattccc
     7561 ggagccatcc gtctgcacga cggcaatgtg caggagttcc tgcagagcgc caataagtca
     7621 aaagaggtga ttgtctactg ctatcatggg aacagcagtt tgggtgcgac tgcctttctt
     7681 atcgagaacg gcttcacgaa tgttgcgagc atgagcggcg gtttcgaggc atggcggcag
     7741 gtgtacgagc atgaataagg ccagagtgtc tagtctctgg ttcccaattt cgagttgacc
     7801 gcaacaagaa agggaacttg gaacgatgaa cttcgaggag gttccatgag cgtcgtcgtc
     7861 ggaagcggtg attacaagta tgaagtaatc gaaaactggg gcacgttgcc cgacggatgg
     7921 actttccatg aagtggcagc cgtggccgtg gatgctcagg atcaggttta ctgttttaca
     7981 cgaggaaaac atccggtgat cgtctttgat cgagacggta attttctgcg cgcttggggc
     8041 gacggtgttt ttaaaagggc gcacggcgca accataggtc ccgatcaaac gatctttttg
     8101 accgacgatg gcgatcacac ggttcggcag tgcaccctcg agggcaaaat cctcatgacg
     8161 atcggcgttc caggaaaatg cgcaacctac caatgcggag aacccttcaa tcgctgcacg
     8221 catgtcgcct tctcggctaa aggtgagatt tacgtctcgg acgggtacgg gaactcgcgc
     8281 gtgcacaaat attcgccgga tggaaaatta ttgttctcct ggggcgaatc gggctctgat
     8341 cccggacaat ttaacattgt tcacaatatc gtcacggaca agaacgggtg ggtctatgtc
     8401 gctgaccggg aaaacaatcg catccaggtt ttcgacgcta acggcaaata cgagacgcag
     8461 tggaacaata tgcatcggcc ctgcgcgctc tacatggaca ccaccaagaa cgatccgatc
     8521 tgctatgtgg gcgagcttgg tcccggtttg ggcgtgaaca aggatgcgcc gaacttgggg
     8581 aatcgcgtcg atatttacga taagacggga aaacgactcg cgcgtttggg cgatatatgg
     8641 cctggcgaaa aacccggcca gttcattgcg ccg
//
LOCUS       DASULZ010000118         3094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_216643, whole genome shotgun sequence.
ACCESSION   DASULZ010000118 DASULZ010000000
VERSION     DASULZ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216643"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VFY96_02415"
     CDS             complement(<1..354)
                     /locus_tag="VFY96_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5605336.1"
                     /translation="MALVIDRLRTVECEFWADAFCRLAPVHAERAAKAEAAGDSATAR
                     KEYVIAYGCYHVARYPAPNSPAKMAAYWKSQEYFYKAARYFDPPVERVEMPFHGRAGE
                     GSVSIGLLRKPRESLN"
     gene            680..1453
                     /locus_tag="VFY96_02420"
     CDS             680..1453
                     /locus_tag="VFY96_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5605337.1"
                     /translation="MARDVAGRVLPPVLAVASVIAIWWSLYLIYPRLLPGPMNTLQEA
                     LRLVSDGTFFFHMYQSLRRVFVGAVVAMFFSVGVGIYMGTVAMGERFFQPLVVLGLTI
                     PGLMWALIAVMLFGINEFSPYFAVTVTIFPMLVINIWAGVKSLDKELIDMSHVFRFTK
                     WMKISQVILPQLVPNIFAATRYGLGLAWKVVVVVEMFGTSNGVGYQVMKSYQVFNMEG
                     VIAWTITFVIAMIVIEYGLINFAERRLTAWRPRVEVWRR"
     gene            1453..2214
                     /locus_tag="VFY96_02425"
     CDS             1453..2214
                     /locus_tag="VFY96_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5605338.1"
                     /translation="MAELWVKDAEKWFRREDGRPFKIIDRLSFAAREGCVTVLIGPSG
                     CGKSTLLNSVVGLENLDRGELIFINRGKPEQRPLIGYVFQSPRLLPWKTVGENITLAL
                     QGAGVPRSQWESRVTRYLNLVGLEEYANQFPLYLSGGQRQRVGLARALAIESDIVLMD
                     EPFASVDELTARQLRQTTRRLCEQLHRTVVFVTHNLAEAAYMGDYVVTLTQRPARLDA
                     YVPNPLPSPHHWGAAIYKFAAELENKVTLKEDTET"
     gene            2211..>3094
                     /locus_tag="VFY96_02430"
     CDS             2211..>3094
                     /locus_tag="VFY96_02430"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX5605339.1"
                     /translation="MTATRLPLILPLCLFAAFLPARARAGQALTPINIATSAAGQSGY
                     IAALIENKKLDTKYGIKITNMMMDFTEAANAVKLGRTLASSMQPSTAVNLVKSGTDIR
                     LITPQIWSGNSWVVRKDSPYKSFADLKGKKLGNFARVTGAYFFSAVIAKEQKLDIEKD
                     FQNVPAETGALIALLERGEVEAINMFEPHTTRLVMSGKYRVLLDFDIELENIFGAPPL
                     KSSVGILKETVEKQPLLVKAIRSAYVDAIHIIKTGQDEDFFKSKAKELFNLSTPEEVA
                     AGFKQNQANAADKWGESF"
BASE COUNT          678 a          871 c          847 g          698 t
ORIGIN      
        1 gtttaacgac tcccggggtt tgcgcaaaag tccgatcgag acgcttccct cccccgcacg
       61 gccatggaat ggcatttcga cgcgctccac aggtggatcg aaatagcgcg ccgctttgta
      121 aaaatactcc tgtgatttcc aatacgcggc catcttcgcc ggagaattgg gcgcgggata
      181 gcgcgcgaca tgataacagc cgtaggcgat cacgtattct ttgcgcgcag tcgcgctgtc
      241 gccggccgct tccgcttttg ccgcccgctc ggcgtgcacc ggcgccagcc ggcagaatgc
      301 gtcggcccag aattcgcact caaccgtgcg caatcgatcg ataaccagtg ccacttcttt
      361 aaacacggtg tagagaaacg ggttgcgatt ttctctagcg cgctcccaaa agctcgtttg
      421 atttcgtcga tagggcgggg agcaaacgat tcgcttgggc tagtcatgaa cgcctccgat
      481 agctaaattg acaaaaactg agcaaagcag cggcgcaagt caaaacaatc cgccgccggg
      541 cgcttaaacg gattgacaag aatcgccgtt ctatagcact acattacctg tgaagtctga
      601 gcccgatcat tcagctcttg cggcgggccg gcaggtcggc attgccggag ccgaaaccct
      661 cccgcccttt gaagctcgaa tggcacgcga tgtggcgggc cgcgtcctgc cgccggttct
      721 agcagtggcc agtgtcatcg caatctggtg gtctttgtat ttgatctacc cacgcctgct
      781 gccgggtccg atgaacacgc ttcaggaagc tcttcggctc gtgagtgatg gcaccttctt
      841 ttttcacatg tatcaaagct tgcgccgtgt tttcgtcggc gccgtggtcg caatgttttt
      901 cagcgtcggt gtcggcattt acatgggcac ggtcgccatg ggcgagcgtt tttttcaacc
      961 cctggtggtg ctcggcctga ccattcccgg cctgatgtgg gcgcttattg cggtcatgct
     1021 attcggcatc aacgagtttt ctccctattt tgccgtgacc gtaacaatct ttccgatgct
     1081 cgtgatcaat atttgggccg gagtcaagtc cctcgataaa gagctcatcg atatgagcca
     1141 tgtcttccgc ttcacgaaat ggatgaagat ttcccaggtg attttgccgc agctcgtgcc
     1201 gaatattttc gcggcaaccc gttacggtct cggtctcgcc tggaaagtcg tggtcgtcgt
     1261 cgagatgttc ggcaccagca acggcgtcgg ctatcaagtg atgaaatctt accaagtctt
     1321 caacatggaa ggcgttattg cctggacgat aacttttgtc atcgccatga tcgtaatcga
     1381 atacggtctg atcaacttcg cagagcgccg cctcaccgcg tggcggccac gagtcgaggt
     1441 ttggcggcgc taatggccga gctttgggtc aaagacgccg aaaagtggtt ccggcgcgag
     1501 gatggccgac cgttcaaaat tatcgaccgc ttatctttcg ccgcacgcga aggctgcgtc
     1561 acggtattga tcggcccgtc gggatgcggc aaatcgacgc tgctcaattc cgtggtgggc
     1621 ttggaaaatt tagatcgtgg agagctcatt ttcattaatc gcggcaaacc cgagcagcgg
     1681 cccctgatcg gctacgtctt tcaaagcccg cgtttgttgc cttggaaaac tgtcggcgaa
     1741 aatatcactc tagccttgca aggcgccggt gtgccccgaa gccaatggga atcgcgcgtc
     1801 acacgctatt tgaaccttgt cggcctcgaa gaatatgcca accaatttcc gctctacctt
     1861 tcaggtggac agcgccagcg ggtcggactg gcgcgcgccc tcgcgatcga gtcggatatt
     1921 gtcctcatgg acgagccgtt cgcaagtgtg gatgagctta ccgcacggca actacgccaa
     1981 accacacggc ggctgtgtga gcaactccac cgcacggttg tttttgtcac acacaatctc
     2041 gccgaagcgg cttatatggg cgattacgtc gtgacattga cacagcgtcc ggcgcggctc
     2101 gatgcttatg tgcctaatcc gctgccgtcg ccacaccact ggggcgccgc tatttacaaa
     2161 tttgccgccg agctcgaaaa caaggtcact ttgaaggagg ataccgaaac atgacagcaa
     2221 cccgactgcc tttgattctc ccactctgcc tcttcgcggc attcctacct gcgagggctc
     2281 gggccggcca agccttgacc ccgattaaca ttgcgacgag cgccgccggt caatccggct
     2341 acatcgccgc cctcattgaa aacaaaaagc tcgataccaa gtacggcatc aagatcacaa
     2401 atatgatgat ggatttcacc gaagccgcca acgcggtcaa gcttggtcga accctagcca
     2461 gctcgatgca accgagcacg gcggtcaacc tggtgaagag cggcacggat attcggctga
     2521 ttacgccgca aatttggtca ggcaattcct gggtggtgag aaaagattcg ccgtacaaat
     2581 cgttcgccga tctgaaagga aaaaagctcg gcaactttgc tcgcgtgacg ggcgcctatt
     2641 tcttctcggc ggtgatcgcc aaagagcaga agctcgacat tgagaaggat tttcagaatg
     2701 ttccggctga aaccggcgcg ctgatcgcgc tcttggagcg tggcgaagtc gaggcgatca
     2761 atatgttcga gccacacacc acgaggttgg tgatgtccgg caaatatcgc gtgctcctcg
     2821 acttcgatat cgaactggag aatatttttg gcgcgccacc actcaaatcg agcgtcggca
     2881 tactcaagga aaccgtcgag aagcagcctc tgttggtcaa ggcgattcgt agcgcctatg
     2941 tcgacgcgat tcacataatt aaaacgggac aggatgagga ttttttcaaa tcaaaagcaa
     3001 aagagctttt caatttgagc acgcctgagg aagtcgccgc cgggttcaag cagaatcagg
     3061 cgaacgccgc cgacaaatgg ggcgagagct tctt
//
LOCUS       DASULZ010000119         3937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_318525, whole genome shotgun sequence.
ACCESSION   DASULZ010000119 DASULZ010000000
VERSION     DASULZ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318525"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VFY96_02435"
     CDS             complement(<1..510)
                     /locus_tag="VFY96_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyalkane--coenzyme M transferase"
                     /protein_id="HEX5605340.1"
                     /translation="MKRSIERILTTHVGSLPRPADLRAMWAKKNPTAEDEAALQTRLH
                     SAVGEVVRMQKDVGIDIPNDGEFGKPMRASTDRGAWGNYIFDRLSGFTATSPETIAPD
                     TAAPGAPMRIVGVRWEQREFAEFYADTGLGTPSTAALRPTCTGPIAYSAEQFLRDQLV
                     NLKLAANAAG"
     gene            complement(600..1502)
                     /locus_tag="VFY96_02440"
     CDS             complement(600..1502)
                     /locus_tag="VFY96_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605341.1"
                     /translation="MGSVLSLAKGWHTPGILLAFLLVILLQAQVGWAQELAPPKLVEE
                     LGKQDTIYRSHGADVPSGYVTNRGLSDYAGLLPSGFCAALGSLGNSDQWLDIGAGDGQ
                     AILDYYAAQADAAAVEKCGRSGPRARAVALSIEDRRTKSWQLQAVMLSGDRIRYLSGK
                     RLRDYSREDLGKFQIITDVYGGFSYTEDLSRFIDKVLSLLEVGGAFYTLVQNVQLESG
                     KGKPSSSYQTELVDSAGRDVRVCSWLKQTPCVNVGCESKSDWDTPTELINIRKVCSDV
                     AVPRLKLLKYEAGNPPGRRFQLEP"
     gene            complement(1749..2393)
                     /locus_tag="VFY96_02445"
     CDS             complement(1749..2393)
                     /locus_tag="VFY96_02445"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5605342.1"
                     /translation="MEIALYYAPNTCALAPYVTLTEAGADFEVRALNFAKRQNFSPEY
                     LKINPKHKVPVLVVNGKILTENVAIHQWVHRNFPNAKILPANPWDELQAISLHAWCAS
                     GIHPYLSRINNPAKVCDAPGASDRVIEIATEFLFENFKIADEMLADREYFFDHFTAPD
                     AHFFWCCRRATQLKVDISGFSNVVSHFKRMQERPSVKKLLAYEKQVNEGFAKTA"
     gene            complement(2481..3842)
                     /locus_tag="VFY96_02450"
     CDS             complement(2481..3842)
                     /locus_tag="VFY96_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605343.1"
                     /translation="MADKNSWQPPEFVSRDRCVKDSEEILGMPDLPIRSTEDIFRIST
                     LGMDWDLGMIVHEPVDSRKIPTGADGKKAGFFLLHGGSSDFKGIERHAKLLAQKFGFR
                     VIAGTFPGRFYFPDTSRDWPDDTIHADGTVRTPIWKQGELITPEQYDVVKDPSKRNRY
                     GTRTLARAKPETNFWFRMAAWPAAFEAGMIEACRRHFPEKEFSVYGQGHSTGGPFVCM
                     LSQRIPNMAGVCATEHSPFGYLCCDRDKWGGDMGKITGYEKPEKKGQGRNDPFNELYI
                     RTWRDLARYQGPEALGQEGPTALMRLPAIMEDILDAWEKAKNRPQFKAEYVVTHAITD
                     SLAEAARVSAARLKMNRDETEALVNRFIGYTRELRGTDDKPVPPFLFEISKDSRDHSP
                     EVYEEVILPGFRAMNPAPKIGITRFGAGVHSFWKAEKDLPVGIVPSVIKSWKEAVTGG
                     YFI"
BASE COUNT          773 a         1115 c         1148 g          901 t
ORIGIN      
        1 gccggcagcg ttggcggcaa gcttcaagtt aacgagctgg tcgcgcagaa actgctctgc
       61 gctgtaagcg atcgggccgg tgcaggtcgg gcgcagcgcg gcggtgctgg gcgtgcccaa
      121 gccggtgtcg gcgtaaaatt cggcgaactc gcgctgttcc cagcgcacgc cgacgatgcg
      181 catcggcgcg ccaggggcgg cggtgtcggg tgcgatggtc tccggcgaag tcgccgtgaa
      241 gcccgagagg cggtcgaaga tataattgcc ccaggcgccg cgatcggtcg acgcgcgcat
      301 gggcttaccg aattcaccgt cattggggat gtcaatgcct acatctttct gcatgcgcac
      361 gacttcgccg accgctgagt gcagtcgggt ttgcagcgct gcttcgtcct ccgccgtcgg
      421 attttttttt gcccacatgg cgcgcaaatc cgccggccgc ggcaggctgc cgacatgggt
      481 ggtgagaatg cgttcaatgc ttcgtttcat aagccgcttg cctttcacaa ttgggccagc
      541 atgctaaacc cggaactgag agctgcgtgg caacggtgac tgctgtgatt tcgaccatcc
      601 tacggctcca attggaatcg tctgccggga ggattccccg cctcgtattt caataatttc
      661 aagcgcggga ccgcgacgtc gctgcaaact ttccgaatat ttattagctc ggtgggtgtg
      721 tcccagtcgc tcttcgactc gcagccgacg ttcacgcagg gggtctgctt cagccacgaa
      781 cacactctta catcgcggcc ggctgaatcc accaactcgg tttgatacga ggagctgggt
      841 ttgcccttgc cactctctaa ctgcacgttt tgcaccagtg tatagaaggc gccgccgacc
      901 tccagcaggc tcagcacctt gtcgatgaat cgagacaagt cctcggtata agagaatccg
      961 ccgtagacgt cggtaatgat ctggaatttt cctaggtcct cgcgtgaata gtctcgcagc
     1021 cgcttgccgg agagatagcg tatacggtca ccgctgagca tcaccgcctg cagttgccag
     1081 ctcttggtcc ggcggtcttc tatcgacagt gcgacggcac gcgccctggg accggaccgg
     1141 ccgcactttt caaccgccgc cgcgtcggcc tgcgctgcgt agtagtccag tatcgcctgt
     1201 ccgtcgccag ccccgatatc gagccattgg tccgagttgc ccaggctgcc gagcgcggcg
     1261 cagaagccgg agggaagaag acccgcgtaa tccgagagcc ctcggttagt gacgtagccg
     1321 ctgggaacgt cggcgccgtg gctacggtag atcgtatcct gtttgcccaa ttcctcgacg
     1381 agtttcggtg gagcaagttc ctgtgcccag ccaacttgcg cttgcagaag gatgacgagc
     1441 agaaatgcta ggaggattcc cggcgtatgc catccttttg ccaacgataa gactgaaccc
     1501 atgaatttgc ttcctccttg cgtgaaacac atcgttgtta agcccgcttt ctgctgtccg
     1561 aggcctgttt gatgccgccg gttgctgcaa ggctgaagtc actgcgctga cgaaagcgct
     1621 ctgcacttcc tccgtgtaaa tcggagttgt cctcgattgc ggcgcccttt tcgcggagac
     1681 ccttcattga cttcgaaggg cagggcgaaa tgatctgctt ctcggcaaca aaattttgtg
     1741 caactcacct aggccgtttt cgcaaagcct tcattcactt gtttttcgta ggcgagcagc
     1801 ttcttgacgc tcgggcgctc ttgcatgcgt ttgaaatgcg aaacgacgtt ggaaaaacct
     1861 gaaatgtcca ccttcaactg ggtggcgcgg cggcagcacc agaaaaaatg cgcgtcgggg
     1921 gcggtgaaat ggtcgaagaa gtactcgcgg tccgctagca tctcgtcggc gattttgaaa
     1981 ttctcgaaca aaaattccgt cgcgatctcg attacgcggt cggacgcgcc cggcgcgtcg
     2041 cagactttgg ccgggttgtt gatccggctc aggtagggat gaatgccgct ggcgcaccag
     2101 gcgtgcagcg agatcgcctg caattcatcc cagggattcg cgggcaggat cttcgcgttg
     2161 ggaaagtttc gatggaccca ttggtggatg gcgacgttct cggtcagtat tttgccattc
     2221 acaacgagca caggcacttt gtgcttcggg ttgattttca ggtattcggg cgagaaattc
     2281 tgccgcttgg caaaattaag cgcacgcacc tcgaaatcgg cgccggcttc ggtcaaggtg
     2341 acatagggag cgagcgcgca ggtgttcggg gcatagtaaa gagcgatttc catttacgtg
     2401 tcctctttaa ttttgattga gaagaagcgt gcggcacacg aaccacatac ccaatcaacg
     2461 cacgtctaaa tttccattga tcaaatgaaa taaccgccgg tcaccgcttc cttccacgat
     2521 ttgatcaccg aaggcacgat gccgaccggc aagtcttttt cggctttcca gaaactatgc
     2581 acaccggcgc cgaagcgagt gatgccgatc ttgggcgctg gattcatcgc gcgaaagccg
     2641 ggcaggatca cttcttcgta aacttctggg ctgtggtcgc ggctatcttt ggaaatttcg
     2701 aaaagaaacg gcggtaccgg cttgtcgtca gttcccctca attcgcgcgt gtagccaatg
     2761 aagcgattca ccagcgcttc ggtttcgtcc cggttcatct taagtcgcgc ggcactgacg
     2821 cgagccgctt cggcgaggga atccgtgatc gcatgggtga cgacgtattc ggccttgaac
     2881 tgcggtcggt ttttggcctt ttcccaagcg tcgagaatat cttccatgat cgcgggcaag
     2941 cgcatgagcg ccgtcgggcc ttcctggccg agagcttcgg ggccttgata gcgggcgaga
     3001 tcgcgccagg tgcggatgta aagctcattg aacggatcgt tgcggccttg gccttttttc
     3061 tccggttttt cgtagccggt aatcttgccc atgtcgccgc cccacttgtc gcgatcgcag
     3121 cagagataac cgaagggcga gtgctcggtg gcgcagacgc cggccatgtt gggaatgcgc
     3181 tgtgacaaca tgcagacaaa cggcccgccg gtggaatggc cctgaccgta cacggaaaat
     3241 tctttttccg gaaaatgacg gcggcacgcc tcgatcatgc ctgcttcaaa cgcggccggc
     3301 caggccgcca tgcgaaacca gaaattcgtt tccggctttg cacgtgccag tgtgcgcgtg
     3361 ccataccggt tgcgcttcga gggatctttg acgacatcgt attgctccgg cgtgatcaac
     3421 tcgccttgtt tccagatcgg cgtccgcacg gtgccgtccg cgtgaatcgt gtcgtcaggc
     3481 caatcgcgac tcgtatcggg aaaataaaaa cggcccggga acgtgccggc gatgacgcga
     3541 aagccaaact tctgcgcgag cagtttggcg tgacgctcga tgcctttgaa atcgctcgag
     3601 ccgccgtgca acaaaaaaaa tccggctttc ttgccgtcag cgccagtggg aattttgcga
     3661 gaatccaccg gctcgtgcac gatcatgccc agatcccaat ccatgccgag agtgctgatg
     3721 cggaaaatat cctcagtgct tcttatgggc aggtcaggca tgccgaggat ttcttcactg
     3781 tctttgacgc agcggtcgcg ggagacaaat tcgggcggtt gccagctatt tttatcggcc
     3841 atttttctct cctctttgtt ctcgttgtac taagcggagc gaatcggtgt caaccatatc
     3901 aatatattga gagactcgcg tctactgtct ggcgtca
//
LOCUS       DASULZ010000120         4008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_14526, whole genome shotgun sequence.
ACCESSION   DASULZ010000120 DASULZ010000000
VERSION     DASULZ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14526"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VFY96_02455"
     CDS             complement(<1..187)
                     /locus_tag="VFY96_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEX5605344.1"
                     /translation="MNYTRSEMVVGLFVVVGLACLGYLAIKLGKLEVFGAGGYTVSAD
                     FPSVAGLKVGDPVEIAGV"
     gene            complement(199..981)
                     /locus_tag="VFY96_02460"
     CDS             complement(199..981)
                     /locus_tag="VFY96_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5605345.1"
                     /translation="MELRDDVGHVVIMIRVVDLHKTFGGQEVLRGINVEFETGKITTI
                     IGGSGSGKTVLLKHLNALLLPDRGSVLVDGRDITKLGQSALNEVRQKFGMLFQSAALL
                     DSMTIYDNVAFPLREKTKLQEKDIEQKANDSLAQVGLAGMGYKFPAEVSGGMKKRAGL
                     ARALVTQPEIMLFDEPTTGLDPLLGKSIHELIRRIHDKFRFTGVIVSHDIPEVFKFSD
                     RVAMLANGVIIEAGTTEEFTASTNPMVKQFLQGETQGPISVL"
     gene            complement(948..1718)
                     /locus_tag="VFY96_02465"
     CDS             complement(948..1718)
                     /locus_tag="VFY96_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HEX5605346.1"
                     /translation="MLSLIGQTGRYATQSTAGMGRIFLFLLSAFAWAFRPPPRVRLVV
                     LHLRTIGVESLSVVILSGVFTGMVMGLQGYYSLRKFNAENFLGSAVALGILRELGPVL
                     SAFMVTGRTGSAMAAEIASMKVTEQIDALYSMAINPIQYLISPRVIAGLISMPLLTAI
                     FDVVAIYGAYLVGVELLGVSSGSYFAGMEQSVVFKDVFSGFLKSVSFGLIITWICCYK
                     GFNAPPMATGVGQATTESVVLCFVMILVWNYVMTSVML"
     gene            1859..2653
                     /locus_tag="VFY96_02470"
     CDS             1859..2653
                     /locus_tag="VFY96_02470"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: protein motif:HMM:NF017755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEX5605347.1"
                     /translation="MMSRSDESLDAFFDGRVTLYQSRTGYRFSLDALLLAHFVTVKTG
                     ARIVDLGTGNGVIPLALAALHPSIKVTGIELQPRMVERARRNIGRNELQTQIKIAHGD
                     VRSPKQIGGAGDFDVAVCNPPYRKSTSGRLSMNEERQVARHEIAGGLDEFLRAGAFFL
                     RANGSMAMVYPAVRSVDLLTAMRHAGLEPKRLRIVHSFRAAKASLILAEAIKGGRSGV
                     EVLAPLSIYEGGKTYSDEVASMIAGTRARLRHAQTGDALEDEERIG"
     gene            complement(2650..3114)
                     /locus_tag="VFY96_02475"
     CDS             complement(2650..3114)
                     /locus_tag="VFY96_02475"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEX5605348.1"
                     /translation="MTKKPPSVDVIGEVLDRSLSRLDLSRRLDEYGVWPIWRDVVGEA
                     IARNAQPEKIRNGTLFVKVSSPVWMQELQFMKEMIAEKLNHRLTGEIVKNIFFMVGKI
                     EATTASVETRNKPTEVPAIPIDEEFLHSIEDPEIRAAFQRLVKSFAHRRAKG"
     gene            3334..>4008
                     /locus_tag="VFY96_02480"
     CDS             3334..>4008
                     /locus_tag="VFY96_02480"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830595.1"
                     /note="catalyzes the reduction of ribonucleotides to
                     deoxyribonucleotides; the rate-limiting step in dNTP
                     synthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEX5605349.1"
                     /translation="MAIGTSQAAEIEARKTTAGAKAGLKISRYFTRPGIQPYDEIEWE
                     LRNASITNEKGKVVFEQTNVEIPKSWSLMATQVVVSKYFRGPLGTPQREHSVRQMISR
                     VVDTITGWGRKDGYFASDDDAQAFADELTHILVHQKACFNSPVWFNCGIEEKPQCSAC
                     FILAVEDTMDSILDWYRKEGVIFKGGSGSGVNLSKIRSSKEQLAGGGTASGPVSFMKA
                     ADASAGV"
BASE COUNT          953 a         1066 c         1114 g          875 t
ORIGIN      
        1 tgactccggc gatctccacg ggatcgccga ccttgagacc ggccaccgag ggaaaatcag
       61 cgctgacagt ataaccgcct gcgccaaaaa cttcgagctt gccgagcttg atggcaagat
      121 aaccaaggca ggcgaggccc accaccacga acaagccaac caccatttcg ctgcgcgtat
      181 aattcacgga tcggactcct aaaggacgct gatcggtcct tgggtttcgc cttgcagaaa
      241 ttgtttgacc attggatttg tcgacgcggt aaattcttcg gttgtgccgg cttcgatgat
      301 aacgccattg gcgagcatgg cgacgcgatc ggaaaacttg aacacctccg gaatgtcgtg
      361 gctgacaatc accccggtga acctaaactt atcgtgaatc cgtcgaatca gttcgtgaat
      421 gctttttcca agcagcgggt ccaaaccagt ggtcggctcg tcgaacagca ttatttcggg
      481 ttgagttacg agagcgcgtg ccagtccggc gcgttttttc atgccgccgc tcacttctgc
      541 aggaaacttg tagcccattc cggcgaggcc cacttgcgcg aggctgtcat tggctttttg
      601 ctcgatgtcc ttttcctgga gcttggtttt ttcgcgtaac ggaaatgcga cgttatcata
      661 gatggtcatt gaatcgagca gcgccgcgct ttgaaaaagc atgccgaatt tttgccggac
      721 ttcgttcaac gcgctttggc cgagcttggt aatgtcgcgg ccgtcgacca gaacgctgcc
      781 gcggtccgga agcaggagcg cgttcagatg ttttagcaat accgtcttcc ccgatccgct
      841 gccgccgatg atcgtggtaa tctttccggt ttcaaattcg acgttgattc ccctgagcac
      901 ctcttgaccg ccaaaggttt tgtgcagatc gacgacgcga atcatgatta caacatgacc
      961 gacgtcatca cgtaattcca aactaggatc attacgaagc aaagcacgac ggattcggtg
     1021 gtcgcttgac cgacaccggt ggccatgggc ggcgcgttaa aacctttgta acagcagatc
     1081 caggtgatga tcaggccaaa gctcacggat ttcaaaaagc cgctgaaaac atccttgaag
     1141 accacactct gctccatgcc cgcgaagtaa cttcccgagc tgacgccgag taactcgacc
     1201 ccaaccaagt aagcaccata aatcgctacc acgtcgaaaa tcgcagtcag caacggcatg
     1261 gaaatcaagc cggcgatgac acgtggcgat atcagatact ggatcggatt gatcgccatc
     1321 gagtagagcg cgtcaatttg ctccgtaact ttcatgctgg caatttccgc tgccatggcg
     1381 gagccggttc tgcccgtgac catgaaggcg gagagcaccg ggccgagctc gcgcagaatg
     1441 ccaagagcaa ccgccgaacc gagaaaattt tcagcgttga actttctcaa gctgtaatag
     1501 ccttgaaggc ccatgaccat gccggtaaaa actcccgaaa ggataaccac cgaaagcgat
     1561 tcgacgccga tggtgcgcag atgaagaacg accaagcgta ctcgcggcgg cggccgaaat
     1621 gcccaggcga atgccgacag cagaaacaaa aaaattcgtc ccattccggc cgtgctctga
     1681 gtcgcgtaac ggccggtttg gccgattaga gaaagcataa ttgcgcgaag gtctttgtgt
     1741 tattagactc aggctattac agaatagttg gcgcgttttc aagaatcaca atcatttatt
     1801 caagatctct tgcggggttc cggctgtgcc ggtttgcggc tgccgcggac tgcgatggat
     1861 gatgagtcga tccgacgaaa gcttggatgc attttttgac ggccgggtaa cgctctatca
     1921 aagccgtacc ggctatcgct tttcactgga cgctttgctg ctggcgcatt ttgtcacggt
     1981 aaagaccggt gcgcgaattg tcgatttggg aactggcaac ggggttattc cgctggcgct
     2041 cgcggctctc cacccatcga tcaaagtgac cgggatcgag ttgcagccca gaatggtgga
     2101 gcgagcgcga cgcaatattg gccggaacga gcttcaaacc cagatcaaaa ttgctcacgg
     2161 cgacgtgcga agtccaaaac agatcggggg cgccggagac tttgacgtcg cagtctgcaa
     2221 tccgccctat cgaaaatcca cgagcgggag attgagcatg aacgaagaac ggcaggtcgc
     2281 gcgtcatgaa atcgccggtg gactcgacga gtttcttcgc gccggagcgt tctttttgcg
     2341 agcgaacggt tcaatggcaa tggtttatcc cgccgtgcgg agcgtggatc tgttgaccgc
     2401 gatgcgtcat gcgggtctag agccgaagcg cttgcgcatc gtgcattcct ttcgcgccgc
     2461 gaaagcttca ctgatcctcg ccgaagcgat caaaggcggg cgcagcgggg ttgaggtgct
     2521 agcgccgctt agcatctacg aaggcggtaa aacctacagc gatgaagtcg cgtcgatgat
     2581 cgccggcacc cgggcgcgcc tgcgccacgc tcaaaccggc gatgcattgg aggatgagga
     2641 gcgaatcggt tagcccttgg ctctgcgatg agcgaagctc ttgacgagcc tttgaaatgc
     2701 ggcgcggatt tccggatcct cgatcgagtg aaggaactct tcatcgatcg gaatggctgg
     2761 aacttcggtg ggtttgttac gtgtctccac tgaagccgtc gtggcctcta tcttgccaac
     2821 catgaaaaaa atgtttttga cgatctcgcc ggtgagccga tggttgagct tctcggcaat
     2881 catctctttc atgaactgaa gctcctgcat ccacaccgga ctcgatactt tgacgaacag
     2941 cgtgccgttg cgaatttttt ccggctgcgc attgcgcgca atggcttcgc cgaccacatc
     3001 gcgccagatg ggccaaaccc cgtattcgtc gagccttctg gagagatcaa ggcggctgag
     3061 cgatctgtcc agaacctcgc caatgacgtc tacggacggc ggctttttcg tcaaggatgc
     3121 cacgacgcga cattagcaga agtaaatcgt cgaaaccagc cgcaaattga ccgttggagg
     3181 ccgtgcaaaa atctctaaac tcggcgaaaa atcaggacaa aaaacgatga gaaaaattct
     3241 tattgacaaa ctgaacgttt gtttctaaaa gtacaaaatc tagtgtccgt ctgactcaag
     3301 cacacaatat attgcgcgaa gggagaatcg aaaatggcta ttggcacgtc gcaagcggcc
     3361 gagatagagg cccggaaaac taccgccgga gcaaaggccg ggctgaaaat atctcggtat
     3421 tttacccgtc cagggattca gccgtatgac gagatcgagt gggagttacg gaatgcgtcg
     3481 atcaccaatg aaaagggcaa ggtggttttc gaacagacca acgtcgagat acccaaatcg
     3541 tggtcgctca tggccactca agtggtggtt tccaaatatt ttcgcggacc cctgggcacg
     3601 ccgcaacgag agcatagtgt gcgccagatg atcagccgag tggtggatac cattacgggg
     3661 tggggtcgta aggacggcta tttcgccagc gacgatgacg cccaggcctt tgccgacgag
     3721 ctaactcata ttctggtgca ccaaaaagcc tgctttaaca gccccgtatg gtttaactgc
     3781 ggaatcgagg agaagccgca gtgctcggct tgttttattc ttgccgtgga agacaccatg
     3841 gattctattc tcgattggta tcgcaaagaa ggagtcatct tcaaaggtgg ctccggttcc
     3901 ggcgtcaatc tgtcaaagat tcgctcatcg aaggaacagc tggccggcgg tggcacggca
     3961 tcgggacccg tctctttcat gaaagcggcg gacgcttcgg cgggcgtc
//
LOCUS       DASULZ010000121         4748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1232112, whole genome shotgun sequence.
ACCESSION   DASULZ010000121 DASULZ010000000
VERSION     DASULZ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232112"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..869)
                     /locus_tag="VFY96_02485"
     CDS             complement(9..869)
                     /locus_tag="VFY96_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5605350.1"
                     /translation="MLSNRVSEIMTTQLTKAPVSASIFEIMQTMIAEDVGRIIITDDE
                     VPVGIFTEKDVVKRVVNAGLDPRKENIKKVMTSPIRAVREETHIIEAFGKMYRGKYRH
                     LLVRGRRGKIIGIVSMRRILNLAVELGQGMAETKTLGEIATAELVTIDETAPVQEAIT
                     SLSDKNVTAVIVTAAGKPTGIFTERDVLKRVAARELNPREISIKEVMTMPIIMMPGST
                     FVGDVLAEMYHRDIRNMPVNNDQGELTGLVSMPEILQFARAFNIDEQVRRSWKEVAEY
                     LDTEDQYTPG"
     gene            1055..1369
                     /gene="clpS"
                     /locus_tag="VFY96_02490"
     CDS             1055..1369
                     /gene="clpS"
                     /locus_tag="VFY96_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEX5605351.1"
                     /translation="MSRRDPEFEHGLVTETKKKVKRPPLYKVLLHNDDYTTKEFVVQV
                     LQYVFHKEQTEAVQIMLHVHKKGIGVAGVYTYEVAETKVALVENLARQHEYPLKCTME
                     EA"
     gene            1371..3638
                     /gene="clpA"
                     /locus_tag="VFY96_02495"
     CDS             1371..3638
                     /gene="clpA"
                     /locus_tag="VFY96_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEX5605352.1"
                     /translation="MISKELEATLNFALKEAKARRHEYVSLEHLLYALLRDKDGKAAI
                     LACGGDIEKLNRGLEEFMTGRMETLPQGRDRDPQQTLSFHRVLQRAVIHAQSAERKEI
                     NGGNLLIAMYREPDSYAVYLLEEQGITRFDLVNYVSHGVSKIAAEEEADSQSEDDEES
                     GEERPSRRVKPLEAFTVNLVDKAAQGLIDPLIGRDDEIERTIHVLCRRRKNNPIYVGD
                     PGVGKTAIAEGLAMKIHHGEVPDALKSAVIYALDMGALLAGTKFRGDFEARLKGVLTG
                     LKKHPNSILFIDEIHTIVGAGATSGGSMDASNILKPALASGELKCIGSTTYHDYKSYF
                     ERDRALARRFQKIEVPEPSQEDAVKILQGLKPHYEKHHGVRYSNSAIRAAVKLSAKHI
                     NDRRLPDKAIDVIDEIGAAVKIQPEEKRRKTISPKDVENIVAKIAKIPPRSVSSSDKE
                     QLQNLDRDLKLVVFGQDSAIDTLASTIKLSRSGLGHPEKPIGCFLFAGPTGVGKTELA
                     KQLAKTMGIEFLRFDMSEYMEKHTVSRLIGAPPGYVGFDQGGLLTDAINRNPYAVLLL
                     DEIEKAHPDLFNILLQVMDHAALTDNNGKKADFRNIVLIMTTNAGAREMSGSPLGFGA
                     QSNVGKGKEAIEKMFSPEFRNRLDAMITFNSLSQENIERVVDKFIIELDQQLNERKVF
                     LQLTPRARRWFAERGYDPTFGARPMARLIQNEIKRILADEILFGRLQAGGKVEVDEEE
                     GKLTFSYSTPALAAV"
     gene            3679..>4748
                     /locus_tag="VFY96_02500"
     CDS             3679..>4748
                     /locus_tag="VFY96_02500"
                     /inference="COORDINATES: protein motif:HMM:NF027806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX5605353.1"
                     /translation="MENFIIAVFLFLFLLEFCLELILNELNLRYVRARWAERKIPDCF
                     RDKIGAEDYQRSVQYTLARGCFGRWSEIYGRGVTLFVLFSGLLGSLDRTATVVAQHYS
                     LGVYGGGILFCFAVALIFSLASLPTSIYSTFYLEAKFCFNKTTARLYILDKLKALVLG
                     LLIGIPFLFVVLWLMDTMGPYWWIWAFGFILAFQLLMIVIFPTFIAPWFNKFEPLPAG
                     ELRDRILALAKQLGFKTSGIYTMDGSKRSAHSNAYFTGLGRSKRIVLFDTLLEQMTID
                     QGLAVLAHEMGHYKMKHILRMLLVQTLFLLIGLYLLSLLLNYAPLFSAFGLERSNHAA
                     LVLFSLVSGPATFYLAPLMNLLS"
BASE COUNT         1101 a         1246 c         1293 g         1108 t
ORIGIN      
        1 accggcttct agccgggcgt gtattgatcc tccgtatcga ggtattcagc cacttctttc
       61 cagctgcgac gcacttgctc atcaatgtta aaggcgcggg cgaactgaag aatctccggc
      121 atcgagacta atccggtaag ctcgccttga tcattattta cgggcatatt tctgatgtcg
      181 cggtgataca tctcggcgag aacatcgccc acgaacgtcg aacccggcat cataatgatc
      241 ggcatggtca tgacttcttt gatcgaaatc tcacgagggt tgagctctcg cgcggctact
      301 cgcttcagga catcgcgctc ggtgaaaatg ccggtgggct taccggcggc ggtgacgatc
      361 acggccgtga cgttcttatc gcttaggctc gtgatcgcct cctgaactgg ggcagtttca
      421 tctatggtca cgagctctgc agtggcaatc tcgccgagag ttttggtttc agccattccc
      481 tggccgagct cgacggccaa gttgagaatg cggcgcatag agacgatgcc gataattttg
      541 ccgcgccggc cgcgcaccag caggtgacga tacttgcccc gatacatttt tccaaaagcc
      601 tcaatgatat gggtttcctc gcgcaccgcc cgaatcggag aggtcatcac cttcttaatg
      661 ttctccttcc tggggtcgag tccggcgttg acgacgcgct ttaccacgtc tttctcagta
      721 aaaattccca cgggtacttc gtcgtcggtg attataatgc gcccgacgtc ctcggcaatc
      781 atggtctgca ttatttcaaa gatcgaggcg gagaccggag ccttggtcag ctgagtggtc
      841 atgatctcgc tcaccctatt ggaaagcata ggcacctcca aaaatgttta tccgaagacg
      901 cgctctgcac atggcgcaga cgaaatcact atagcattcg ccgctcgcca agttaatcat
      961 ttgcccgatg ggcggtcggc gtttcagatc attgcgagca ggtgtaacgg cgtgttagat
     1021 ttattttcac ctcggttatg ataatgggtc cattatgagc cgcagagatc cggaatttga
     1081 acacggcctg gtcaccgaga ccaaaaaaaa ggtcaagcgg cctcctcttt ataaagtcct
     1141 gctccacaat gacgactaca cgaccaaaga gttcgtagtg caggttctgc aatacgtctt
     1201 ccacaaggag cagaccgagg cggttcagat catgcttcac gtgcacaaga aaggcatcgg
     1261 cgttgccggt gtctacacct acgaggtcgc cgaaaccaag gtcgcgctag ttgaaaatct
     1321 ggcgcgtcag catgagtatc cgctgaaatg cacaatggaa gaggcttagt atgatcagta
     1381 aagaactcga agccacgctc aacttcgcgt tgaaagaagc caaagcgcgc agacatgaat
     1441 acgtcagctt ggaacacctg ctctacgcct tgctgcgcga caaggatggt aaagcagcga
     1501 ttctcgcctg tgggggcgac atcgaaaagc tgaatagagg cctcgaagaa tttatgactg
     1561 ggcggatgga aacgctgccg cagggacgag accgcgaccc gcagcagact ttgagctttc
     1621 accgtgtttt gcaacgggcg gtaatccacg cccaatcagc tgagcgcaag gagatcaacg
     1681 gcggtaattt gctgatcgcc atgtaccgtg agccggattc ttacgccgtt tatcttctcg
     1741 aggaacaagg catcacgcgg ttcgatctgg tcaattatgt ttcgcacggc gtgtcgaaga
     1801 tcgccgcgga ggaagaagcg gatagccagt ccgaggatga cgaggaaagc ggcgaggagc
     1861 gaccgtcgcg ccgagtgaag ccgctggagg cgttcacggt aaacttggtt gataaagcgg
     1921 cgcagggttt gatcgatccg ctcatcggca gagatgacga gatcgagcga accattcacg
     1981 tgctctgtcg gcggcggaag aataatccaa tttatgtggg tgatcccggc gtgggcaaga
     2041 ccgccatcgc cgagggtctg gcaatgaaaa ttcatcatgg cgaagtgccg gacgcgctca
     2101 agagcgctgt catttacgcc ctggacatgg gagccctgct tgcaggcacg aagtttcgcg
     2161 gtgatttcga ggcgcgcctc aagggggttc tcaccggact taagaaacat cccaactcga
     2221 tcctttttat cgatgaaatc cacaccatcg tcggcgctgg cgcgaccagc ggcggctcca
     2281 tggacgcgtc aaacattttg aagcccgctt tggcatccgg ggagctcaaa tgtatcggat
     2341 cgacaactta tcatgattac aagagctatt ttgagcgtga ccgagcatta gcgcgacgct
     2401 ttcaaaaaat cgaagtgcca gaacctagcc aggaagacgc ggtcaaaatc ctgcaaggtt
     2461 taaaaccaca ctacgaaaaa caccacggcg tgcgctacag caacagcgcg atccgcgcgg
     2521 ccgtgaagct ctcagccaag cacatcaatg atcggcgcct gccggacaag gccatcgacg
     2581 tcatcgacga aatcggcgcg gcggtgaaga tccaaccgga agaaaaacgg cgcaaaacga
     2641 ttagcccgaa agatgtcgag aacatcgttg ccaagatcgc caagattccg ccgagaagcg
     2701 tgtcgagctc cgataaagaa cagctgcaaa acttggatcg cgacttaaag cttgtagtat
     2761 ttggccaaga ttcggctatc gataccctag ccagcacgat caagctttcc cgctccggtc
     2821 ttggccatcc ggaaaaaccg attggctgtt ttctttttgc cggacccacc ggggtcggca
     2881 agactgagct ggcgaagcag ctcgcgaaaa ccatgggcat cgagtttctg cgcttcgaca
     2941 tgagcgaata catggaaaaa cataccgtct cgcggctcat cggcgcgcct cccggatacg
     3001 tagggtttga tcaaggtgga ttgctgaccg acgcgatcaa tcgaaatccc tacgctgtct
     3061 tactgctcga tgaaatcgag aaggcacatc ccgacctgtt caatatttta ctgcaggtga
     3121 tggatcatgc ggctctcact gacaataacg ggaaaaaagc agactttcga aatattgtgt
     3181 tgatcatgac caccaatgcc ggggcgcggg aaatgagcgg ctcaccgctc ggcttcggcg
     3241 ctcaatcgaa cgtgggcaaa ggcaaagaag cgatagaaaa aatgttcagc ccggaatttc
     3301 gtaatcggct cgacgccatg atcaccttca attcgctcag ccaggaaaac atcgagcgcg
     3361 tggtggataa gttcattatc gagctcgacc agcagctcaa cgagcgcaaa gtgtttctgc
     3421 agctcacacc gcgcgcgcgc cgttggtttg ccgaacgcgg ctatgacccg actttcggtg
     3481 cccggccgat ggcacgactg attcaaaacg aaatcaagcg gattctcgcc gatgagattc
     3541 tattcgggag gctacaagcg ggcggcaagg tcgaggtcga cgaagaagaa ggcaagttaa
     3601 ccttttccta cagcacgccc gcgctggctg cggtctgagc cggaactctt ccgctcattc
     3661 gagattctat ccgcgcagat ggaaaacttt atcattgcgg ttttcctgtt cttgtttcta
     3721 ctcgaattct gcctcgaact tattctgaac gaattgaatc tgcgttatgt tcgggcgcgc
     3781 tgggcggaga gaaaaatccc ggattgcttc cgcgataaaa tcggcgccga agactaccag
     3841 cgctcggtcc aatatacgct cgcgagaggt tgcttcggcc gttggtcgga aatctacggc
     3901 cgaggcgtca cgcttttcgt tctgttcagc ggattgcttg gcagcttgga tcgaacggcc
     3961 acggtggttg cgcagcacta ttcgttaggc gtttacggcg gagggatttt gttctgtttc
     4021 gcggtcgcgt tgatcttttc actggcgagc ttgccgacga gtatttactc caccttttat
     4081 cttgaagcaa aattctgctt taacaagacg accgcacggc tttatattct agataaactc
     4141 aaagcgctcg tgctgggcct actgatcggg ataccgttct tattcgttgt gctctggttg
     4201 atggatacga tggggcctta ctggtggatt tgggcctttg gctttattct agccttccag
     4261 cttctgatga tcgtcatatt tcctactttc atcgcgccct ggtttaataa gttcgagccg
     4321 cttcccgcag gcgagttgcg cgatcgaatc ttggcgttgg cgaagcagct cggtttcaaa
     4381 accagcggaa tttacaccat ggacggctcc aagcgctcgg ctcattcgaa tgcgtacttc
     4441 acgggtttgg gtcgttccaa gcggattgtt ctgttcgata cattgctcga gcaaatgact
     4501 atcgatcagg ggttggcggt gctcgcccat gaaatggggc actacaaaat gaagcacatt
     4561 ttgcggatgc ttcttgtgca aacgctcttt cttttgatcg ggctatacct gctgagtttg
     4621 ctgctgaatt atgcgccgct gttttcggcc ttcggtttgg aacggtcgaa tcatgcggcg
     4681 ctggttttat tcagcttggt atctggaccg gcgactttct atcttgcacc gctgatgaat
     4741 cttctgtc
//
LOCUS       DASULZ010000122         2661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1232666, whole genome shotgun sequence.
ACCESSION   DASULZ010000122 DASULZ010000000
VERSION     DASULZ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232666"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VFY96_02505"
     CDS             complement(<1..496)
                     /locus_tag="VFY96_02505"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX5605354.1"
                     /translation="MHEIVVPNQASDKKLENFLKREFPIGYVRKLLRKNGVRVNGRRA
                     KGEDLVRTGDRVQLYIEFEKKSSRAKTPSTKQVNIIYEDDSLLVINKPAGIAVHEGKS
                     VRKGDSVLGMLEANYRGTDIRPQLVHRLDKNTSGLLLVAKNPATAEELENRFEQGAVE
                     KDYFC"
     gene            628..1716
                     /gene="pheA"
                     /locus_tag="VFY96_02510"
     CDS             628..1716
                     /gene="pheA"
                     /locus_tag="VFY96_02510"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEX5605355.1"
                     /translation="MRLSLSRNEPVDVLRNKIDQIDAKILSLLNDRASLAQKIGRSKS
                     VNHQEVYAPHREKEVLKRVCELNRGPLSAQVVGAIFREIISGCRGLEGRLNVSFFGAE
                     ATYTHLATREKFGSSANLRATASIPEVFQEVTRGRADFGVVPIENSTEGAVTHTFDLL
                     VDTDLQICAEIFLEIHHSLLSRSGRAADVKGIISHPQALAQCRGWLARHFPSIPIDEV
                     ASTAHAAMKAAADGSLAAISSVMAREVYDLQTVSANIEDQSNNITRFVVIGKLLTKPS
                     GDDKTSLVFSVQDKPGVLHRMLQPFARSRINLTKIESRPIKNKPWEYLFFLDLRGHRE
                     QNTVKKAIAGLEKNCLFLKILGSYPSGR"
     gene            1713..>2661
                     /gene="hisC"
                     /locus_tag="VFY96_02515"
     CDS             1713..>2661
                     /gene="hisC"
                     /locus_tag="VFY96_02515"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX5605356.1"
                     /translation="MKVVEHVPEYIRSLIPYEPGKPIEEVEREYGICGSVKLASNENP
                     LGPSPRALAALRERLAEIHLYPDGDCFYLRRALAQKLGVDPDCLIFGNGSNEIIELAV
                     RTFMRPGDEAVMAEQAFVVYELIVQAVGGKRTIVPLKNFTHDLAAIADAVTPQTRLVF
                     LANPNNPTGTIYGRREWEAFLERISPDVLVIVDEAYFEYVGAADYPNSLDDQARHGAI
                     LTLRTFSKLYGLAGLRIGYGIGAKDIIALMQRVRQPFNVNAPAQWAALAALADAEHVQ
                     RSLETNQQGMQYLQAEFARLGLTYGPSQANFILVRVGNGP"
BASE COUNT          578 a          715 c          736 g          632 t
ORIGIN      
        1 gacagaaata gtctttttcg acggcgcctt gttcgaagcg gttttccagt tcctcggccg
       61 tcgcgggatt cttcgcaacc agcaacaatc cggatgtatt tttgtcgaga cggtgcacga
      121 gttgaggtct gatatctgtg ccgcgatagt tggcctccaa catgccgaga accgaatctc
      181 ccttgcggac ggattttcct tcatgaaccg cgatccccgc aggcttgttg atgacgagca
      241 gcgaatcatc ctcgtagata atattgacct gctttgtgct cggagtttta gcccttgagc
      301 tttttttctc gaactcgata taaagctgaa cgcggtctcc ggtacgaact aaatcctccc
      361 ccttggcccg gcgaccgttg actcgcacgc catttttccg cagtagcttt cgaacatagc
      421 cgatggggaa ctcccttttt aaaaagttct cgagcttctt atcagatgcc tggttcggca
      481 ccacaatctc gtgcatggct tcaattgaat agcggcagcg ccggctctca gtcaagcagt
      541 ggatgtctga ttatttgcaa ttgtctagtt attaagatag cttacgttga cggcgccagg
      601 gcgttgagcc ttgtttggcc gcgccgggtg aggttgtcat tgagccgtaa tgagccggtt
      661 gatgttttaa gaaataaaat cgatcaaatt gatgcaaaaa tcttaagcct tctaaatgat
      721 cgggcgtctt tggcgcaaaa gatcggccgt tccaaaagcg ttaaccatca agaagtctac
      781 gctccccatc gcgagaagga agttctcaag cgcgtctgtg agctcaaccg cggaccactc
      841 tcggcgcagg ttgtcggcgc gatttttcgg gaaatcattt ccgggtgccg aggtctcgaa
      901 ggacgattga acgtctcgtt ctttggcgcc gaggcgactt acacgcatct ggcgacacgc
      961 gagaaattcg gctcctcagc caacttgcgg gcgaccgcga gcatcccgga agtctttcaa
     1021 gaagtcactc gcggccgagc cgatttcggc gttgtgccga tcgaaaactc cactgaagga
     1081 gcagtcactc acacattcga tttgctcgtc gatacggatc tacagatctg cgcggaaatc
     1141 tttttggaga tccatcacag cttgttgtcc cgatccggca gagcagccga tgtaaaaggc
     1201 attatctcac atccacaagc attggcgcag tgccgcggct ggctcgcgcg ccacttcccg
     1261 tcgatcccga tcgatgaggt cgcaagcacc gctcatgcgg caatgaaagc cgctgcggac
     1321 ggttccttgg cagccatctc cagcgtgatg gccagggaag tttacgatct gcagacggtg
     1381 tccgcgaaca tcgaggatca aagcaataat attacgcggt ttgtggtcat cgggaagctg
     1441 cttacgaagc cgagcggcga tgacaaaact tctctagttt tttccgtcca ggataagccg
     1501 ggcgtcctgc atcgaatgct ccagcccttc gcccgaagcc gcattaacct gacgaagatc
     1561 gaatcgcgac cgataaaaaa caagccttgg gaatatctgt tttttctcga tctgcgcggc
     1621 catcgcgagc agaacacggt gaagaaggcg attgcgggcc tggaaaaaaa ttgcttgttc
     1681 ctgaaaatat tgggctctta tccgagcggc agatgaaggt tgtcgaacac gtgccggaat
     1741 atattcgctc gttgattcct tacgagccgg gaaagccgat cgaagaagtc gagcgtgaat
     1801 atggaatttg cggctccgtg aagctggcct ccaacgaaaa tccgttgggg ccgtcgcctc
     1861 gagcgctcgc cgctctgcgc gagcgactgg cggagatcca tctctatccg gatggcgatt
     1921 gcttctactt aagacgcgcg ctggcgcaga agctcggtgt cgatcccgat tgcttgattt
     1981 ttggcaatgg ttccaacgag atcatcgagc tcgcggtgag gacgttcatg cgccccgggg
     2041 acgaagcggt gatggcggag caagcgtttg tcgtttatga gctgatcgtt caagcggtcg
     2101 gcggcaagcg cacgatcgtc ccgctaaaaa atttcactca cgatctggct gcaattgcgg
     2161 acgcggtcac gccccaaacc cgtctggtgt ttctcgccaa tccgaataat ccaaccggta
     2221 cgatttacgg tcgtcgcgaa tgggaggcat ttctcgagcg catttctccc gatgtcctgg
     2281 tgattgttga cgaggcctat ttcgaatatg tcggggcagc ggattatcca aactccctcg
     2341 acgaccaggc gcggcatggc gcgattttga cattgcgcac gttttccaag ctttacgggc
     2401 tggccggttt acggatcggt tacgggattg gcgcgaagga tatcattgct ttgatgcagc
     2461 gcgtacgcca gccgttcaac gtcaatgcgc cggcgcaatg ggccgcgcta gccgctttgg
     2521 ccgatgccga gcacgtgcag cggagcctcg aaaccaatca gcagggaatg cagtatctcc
     2581 aagcggaatt tgcgcggctc ggccttacat acgggcccag tcaggcgaat tttattctcg
     2641 ttcgcgtcgg caacggaccc g
//
LOCUS       DASULZ010000123         2007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_217542, whole genome shotgun sequence.
ACCESSION   DASULZ010000123 DASULZ010000000
VERSION     DASULZ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217542"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..1512)
                     /locus_tag="VFY96_02520"
     CDS             complement(76..1512)
                     /locus_tag="VFY96_02520"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HEX5605357.1"
                     /translation="MSPEISNVRPEPDKILVDLADYAASYRPESREAIDTARVNLMDA
                     LGCGLLALRYPECTKRLGPVVPGAVLRDGARVPGTAWQLDPVHAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILALADYLSRTNQQQLTVSDVLMALIKAHEIQGILALENSF
                     NRVGLDHVLLVRVATAAVTTAMLGGSREQIISAISNAWTDGGALRLYRHAPNTGPRKS
                     WAAGDATSRGVRLALMALRGEMGYPSALSAKTWGFHDVLFAGKPVTLGRPPASYVMEN
                     VLFKISFPAEFHAQTAVECAFQLHDQVRNRLTEIKTVVISTQESALRIIDKSGPLYNP
                     ADRDHCIQYMTAVGLIFGELTADHYEDAIARDPRIDELRAKMTCVEDPQYSKDYLDPE
                     KRSIANAVQVFFNDGSSTAKIAVEYPVGHRRRRAEGIPLLLKKFEANLASRFLPEQCA
                     NILRLCGDSTQLDATAVNKFTDMFAKNV"
     gene            complement(1639..>2007)
                     /locus_tag="VFY96_02525"
     CDS             complement(1639..>2007)
                     /locus_tag="VFY96_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013925447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX5605358.1"
                     /translation="KLVMGFGHRVYKISDPRSDVIKAWSKKLAAAVDDTRLFPVSERL
                     EQVMRREKKMFPNLDFYSATAYHFCGVPTEMFTPLFVISRITGWAAHIIEQRADNRLI
                     RPNADYIGPDPRPYVPIDKR"
BASE COUNT          447 a          544 c          575 g          441 t
ORIGIN      
        1 atttaaagtg atttcgagct taaacttcgg gcgtgccatt aattcgtcgt gacgctggac
       61 gctagaccat aaacattaga cattctttgc aaacatatcc gtaaatttgt ttaccgccgt
      121 ggcatcgagc tgggtgctat cgccgcacag ccggagaata tttgcgcatt gctcaggcaa
      181 aaaccgcgac gccaaattgg cttcgaattt tttcaggagt agcggtattc cttccgcgcg
      241 ccggcgccgg tgcccgacgg gatactcaac agcgattttc gccgtgctcg atccgtcgtt
      301 gaaaaaaacc tgcacggcat tcgcaatcga gcgtttctcc gggtcgaggt agtcttttga
      361 atactgcggg tcctcaacgc aggtcatttt ggcacggagc tcatcaatgc gcggatcgcg
      421 cgctatggca tcttcatagt gatcggcagt gagctcgccg aaaatcaggc ccaccgctgt
      481 catgtactgg atgcagtgat ctcgatccgc cggattataa agcgggccgg acttatcgat
      541 gatccggagc gccgattcct gcgtgctaat cacaactgtt ttgatttctg tcaagcgatt
      601 cctgacctga tcgtgcagct gaaatgcgca ctcgacggcg gtttgcgcgt gaaactcggc
      661 gggaaaggaa attttaaaca acacgttctc catgacatag gatgcaggcg gccggccgag
      721 cgtaaccggc tttccggcga aaagcacatc gtgaaagccc caggttttgg ccgagagcgc
      781 cgatgggtat cccatttcgc ctcgcagggc catcagcgca aggcgcaccc cgcgactggt
      841 ggcatcgccc gccgcccagg atttgcgcgg gccggtattc ggcgcatgcc gataaaggcg
      901 caaggcgcca ccgtcggtcc aggcatttga aatcgcgctg atgatctgtt ctctggatcc
      961 gccgagcatc gccgtggtga cggctgcggt ggcgactcga accagcaaca cgtgatcgag
     1021 accgacgcgg ttaaagctgt tttctaacgc cagaatgcct tgtatttcgt gagctttgat
     1081 cagcgccatc agcacgtcag aaacagtcag ctgctgctgg tttgtgcgcg agagatagtc
     1141 ggcgagggca agaataccgc ccagattgtc ggaaggatga ccccattcgg cggcgagcca
     1201 ggtatcgttg aagtcgagcc aacgaatcat tgcgccgata ttgaaagcgg cgtgaaccgg
     1261 atcgagctgc catgcggttc ccgggacgcg cgcgccgtcc ctcaagactg cgcccggaac
     1321 cacgggtcct aagcgcttcg tgcactcggg atagcggagc gcaagcaaac cgcagccgag
     1381 cgcatccata agattcaccc gtgcggtgtc gatcgcttca cggctctcag gtcggtagct
     1441 cgccgcataa tcggccaaat cgacgaggat tttgtccggc tctggccgaa cgttggaaat
     1501 ttctgggctc atgcgttctt aaccttcaag gttcaagctt caaggcttgt cctgaaccaa
     1561 gtgaaggatc ttttcgccgt gatcggtaga tcgttcgaag ctaggatcgc aaagcgcgca
     1621 ccgaaacttt gcatcgtctt atctcttatc gatcggcaca tagggtctgg gatcggggcc
     1681 gatgtagtca gcattgggac ggatgagacg attatcagcg cgttgttcga tgatgtgcgc
     1741 cgcccagcca gtaatccggg aaatgacaaa gagcggggta aacatctccg tcggaacacc
     1801 gcaaaagtga taggcggtgg cgctatagaa atcgaggttg gggaacatct ttttttcacg
     1861 ccgcatgacc tgttcgaggc gctccgaaac gggaaaaaga cgagtgtcat caaccgctgc
     1921 ggcgagtttc ttcgaccatg ccttgatcac gtcggaacgc ggatcggaaa ttttgtaaac
     1981 ccgatggccg aaacccatga ccagttt
//
LOCUS       DASULZ010000124         4389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1131570, whole genome shotgun sequence.
ACCESSION   DASULZ010000124 DASULZ010000000
VERSION     DASULZ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131570"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VFY96_02530"
     CDS             <1..606
                     /locus_tag="VFY96_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605359.1"
                     /translation="MGAEHQTHDYYMTIGPMFADPMARQLYAEIASIEEQHVTQYESI
                     IDPEETWLEKWLMHEAGEIYNYYSCLTYETDNRVKEIWQRFLDYELGHFHYVLELFQK
                     YEKRDPAVLIPPSLPEPIEYKSHREFVRKVLNEEVDLRARGKQIVGSDEDESEATGRY
                     RAQLNSNGSPSESVAVGYQWKPGTEIADETPDIEKLRKRVA"
     gene            619..1470
                     /locus_tag="VFY96_02535"
     CDS             619..1470
                     /locus_tag="VFY96_02535"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX5605360.1"
                     /translation="MKSKSTEASGREGATNRTGIMFNPELSAELILGAEAATRSGTEG
                     GAEVTDQATYLTETAPIGSLPAMVADDGEANGSISILLDKLGERLAFERQGTRLYQAF
                     VQKVEALGDGASAGPSSEELRHICEEELQHFRLLQKIITELGGDATVQTPSADVVGVL
                     SHGMVQVVSDPRTTIAQCLQAVLTAELADNDGWQMLSALAEAVGNSDLVDQCEKAFEE
                     EQEHLEDVRAWLNEMTANEAANASNADIGKGDAETERADAGTKRRRTSSKSTGSRAKK
                     SKKRRKK"
     gene            2034..2549
                     /locus_tag="VFY96_02540"
     CDS             2034..2549
                     /locus_tag="VFY96_02540"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX5605361.1"
                     /translation="MNETDSYPAQQNVRTVSQLEQAALRSRSVAERVSDVLTKAVGSL
                     SSIVLHLTWFTLWILINTGVLPIVQRFDPFPFGILTLIVSTEGVLLALTILISQNRMI
                     RQADRRAHLDLQISLLDEQETTAVLRILRRMAQHLGVPDGPAEAEVSKLSQQTDIDVV
                     MRNLEEQLPKD"
     gene            complement(2583..4139)
                     /locus_tag="VFY96_02545"
     CDS             complement(2583..4139)
                     /locus_tag="VFY96_02545"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX5605362.1"
                     /translation="MNNMPVDNHFTITRRYPGSAVDYPMLLKRLRSIHYDLAVDVSCS
                     QSAMGAVLVGFSGARLRAGMRGKWDRWLNIKISKSGEINKYQTLAKFLSSLGVRYESP
                     VPAIALSDLERETGQKKLAALTRGRPCAQTVGVFVGGRKSWGKRWSTANFCELITALH
                     AYGLNVITFVGPEEKDTLGLFRDALDQGIPIVFERSLRMFAAMVSNCDLFVTCDSGPM
                     HLASSLGVRTVAIFRYRNFDRWAPPASSTRLVHDPAGSSTGAVLAACCEELSVHEDHP
                     QKSTGHFQKVPNLRSIPVIHQALDRLESAKVLARLLKTGGWLRLGFVLLLVIYALFVP
                     AGVFEEGTLTETFVDIVGGAALILGGLLSLWTISHRPAGERWNRSIRRELIRSGPYAY
                     MQHPLWIAHLLIGTGMIFLLDAYVFIFLLLIIGMVYQRIIEPAEGQFLRKYFGETFDQ
                     YCRTTPKYIPRILPKNLFFGRRVRLREIGPILALILLVFLFESIESPQNRPFISTFYR
                     WLHPMTVRTE"
BASE COUNT         1105 a         1133 c         1228 g          923 t
ORIGIN      
        1 atgggcgctg aacaccagac gcatgattac tacatgacga ttggcccgat gtttgccgat
       61 ccgatggcgc ggcagctcta cgccgaaatt gcttcgatcg aagagcaaca tgtcacgcaa
      121 tatgagtcga tcatcgatcc cgaagaaact tggctggaaa aatggctgat gcatgaggcc
      181 ggggagattt acaactacta cagctgcctc acctacgaaa ccgacaatcg ggtcaaggag
      241 atctggcagc gatttctaga ctatgagctt ggccattttc attatgtctt ggagctattc
      301 caaaaatacg aaaagcgcga tcctgctgtg cttattccac cctcgctacc ggagccgatc
      361 gaatacaaga gccatcgaga attcgtccgc aaggtgttga acgaagaggt cgatctacgc
      421 gcgcggggca agcagatcgt cggtagcgac gaggatgaat ccgaggccac tggacgttat
      481 cgagcccagc tgaattccaa cggatcaccg agcgaatctg tcgccgttgg ctatcaatgg
      541 aagccgggaa cagagatcgc ggacgaaact ccggacatcg aaaagctccg gaaaagagtt
      601 gcgtgattag gaggtacgat gaaatctaag tcaaccgaag ctagcgggcg tgagggcgca
      661 acgaatcgaa caggaatcat gttcaatccg gagctgagtg cggagctgat actgggcgct
      721 gaagcggcaa caagaagtgg taccgaggga ggcgcggagg tcacggatca agcgacttac
      781 ctaactgaga cagcgcccat tggttcgttg cccgcaatgg tagccgatga cggcgaagct
      841 aacggttcga tttcaattct cctcgacaaa ctcggtgagc gcctggcctt cgaacgtcaa
      901 gggacgaggc tatatcaggc ttttgtgcaa aaagtcgaag ccctgggcga tggtgcctca
      961 gcggggccaa gcagcgaaga gctacggcat atttgcgaag aagagctgca gcacttccgg
     1021 ctcctgcaaa aaatcataac cgaactcggc ggtgatgcga ccgtgcagac gccttcggcc
     1081 gatgtcgttg gagtgctctc tcacggaatg gttcaggtgg tcagcgaccc acggacgaca
     1141 atcgcgcaat gcctgcaggc cgtgctcacg gcggaattgg cggacaatga tggttggcaa
     1201 atgctctccg ctctcgccga agcagtcggt aattctgatc tcgtggatca atgcgaaaaa
     1261 gcatttgaag aggagcagga acatttggaa gatgtccgag cctggctcaa cgaaatgacc
     1321 gcgaatgagg cggcgaatgc gtccaacgcg gacatcggga aaggagacgc ggaaacagag
     1381 agagctgacg cgggaacaaa acggcggcga acttcctcga aatcgaccgg ctcccgtgcg
     1441 aagaaaagca aaaagcggcg caagaagtaa tcagccgtgc cgtaggtcgc gcacgattcg
     1501 agagcttcca atggggcagc tacacgatat cggccagtgt catatccgtt tgcgtgctcg
     1561 agagcgtttg cgaaaggttt gttgaacccg gatttgtcca cgaagtgttt catgagctga
     1621 gcgctcgccc taaggaatga aaatggggaa ggatttttgc tgcgctcgaa aggacaacga
     1681 agacatacct gtcgtcccga acccattcga tgtgcttagg gtcaagtccg tgagagatct
     1741 ttcctcgatt atattgtcaa agagcccttc agcattatca tgttagctgt cgtgaatgcc
     1801 cgtcggattg tggtactcga aacgtaaggc ttgccggatt tgagaccttg ccactcattc
     1861 ggcaatccag tgaatcacac catcgccctt gatccgctcg gcctacctgc ttcaataacc
     1921 ttagattgtt ttgcgcttag tatcgcttga acgatctcag gtggtcctct gtcgtggggt
     1981 ttattcaggc aaggagcgtc gagttcaacg aaagaagaag cagtgtttag accatgaatg
     2041 aaacagattc gtacccggct cagcaaaacg tcagaaccgt gtcgcagctc gagcaggcgg
     2101 cgttgcggag tcgtagcgtg gctgaacggg tcagcgatgt tctcaccaaa gcggtaggca
     2161 gcctgagttc gattgtcctg catctgacct ggtttacgct gtggatcttg atcaacaccg
     2221 gagttcttcc catcgttcag cgatttgatc cttttccgtt cggcatcttg actctgatcg
     2281 tttctacgga gggggtcctt ctcgcgctga ctattctgat cagccaaaat cgcatgatcc
     2341 ggcaagctga ccgtcgcgcc catttagatt tgcaaatcag tcttcttgat gagcaagaga
     2401 ccaccgccgt cctacgaatc ctgcgccgaa tggcgcagca tctgggagta ccggatggtc
     2461 cggctgaggc cgaagtgagc aaacttagcc agcaaaccga tatcgacgtg gtaatgcgga
     2521 acctagaaga gcaattgcct aaggattagc gcagtgtgaa ttgtgcagcg ggcgacgaaa
     2581 cctcattccg ttcgcaccgt catagggtga agccaacgat agaacgtact gatgaacggg
     2641 cgattctgtg gcgactcgat gctttcgaac aggaaaacaa gcagaatgag cgccaagatc
     2701 ggaccgattt cacggaggcg gactcgtcgg ccgaagaata agtttttggg gagaatgcgg
     2761 gggatatatt ttggggtcgt gcggcaatac tgatcgaaag tttcaccgaa gtattttcgc
     2821 aggaactggc cttctgccgg ttcgatgatg cgctggtaaa ccataccgat gatcagtaac
     2881 aaaaatataa aaacgtatgc gtccaggaga aaaatcattc ccgtgccgat cagcagatga
     2941 gcgatccaga gcgggtgttg catgtatgca tagggtccgc ttctaatcag ctctcgtcgt
     3001 atggaccgat tccatcgctc gccggctggc cgatgactga ttgtccaaag cgagagtaaa
     3061 ccgccgagga tcagggctgc gccgccgacg atatccacaa aggtttccgt caacgtgcct
     3121 tcctcaaaaa cgccggcagg tacaaacaaa gcatagatca cgagcagcag aacgaaaccg
     3181 aggcgtaacc acccgccggt ttttagcagt cgggcgagca ccttcgcgct ttcgagtcga
     3241 tccagcgcct ggtggataac cggaatggaa cgaagattcg gaactttctg aaagtgcccg
     3301 gtagattttt gcgggtgatc ctcatgcact gatagctctt cacaacacgc tgctagaacc
     3361 gcgcctgtcg agcttcccgc agggtcatgg accagacgcg tgctgctggc cggcggtgcc
     3421 cagcgatcaa aattacgata acgaaagatt gctaccgtcc ggacgcccaa actggaggcc
     3481 aggtgcatcg ggccgctgtc acaagtcacg aagagatcgc agtttgaaac catggcggca
     3541 aacatcctga gcgatctctc gaagacgatc ggaatgcctt ggtccaaggc atcgcgaaac
     3601 aatcctaacg tatccttttc ctccggcccg acgaaagtta tcacgttgag accataggcg
     3661 tgtaatgctg tgatgagctc gcaaaaattc gccgtcgacc atcgcttccc ccaggattta
     3721 cgcccgccaa caaaaactcc caccgtctgc gcgcacggcc gtcctcgagt caatgctgcg
     3781 agtttctttt gacctgtttc gcgctccagg tcggaaagag ctatcgccgg aaccggtgat
     3841 tcgtaacgaa cgccgaggct actcaaaaat tttgctaacg tttgatactt attgatttcc
     3901 cctgatttgg agatctttat gtttagccag cggtcccatt ttcctcgcat tccggcgcgc
     3961 aagcgcgctc ccgaaaaccc cactaggact gcccccatag ccgactgaga gcagctgacg
     4021 tcgacggcca gatcatagtg gatgctgcgc aaccgcttga gcagcatggg ataatccaca
     4081 gcggagccgg gataacgcct cgtgatagtg aagtggttat ccacgggcat gttgttcatg
     4141 aggttcttcg aaatcggtcc tccgacaaaa tctatgcgcg cgtgtggaaa ttgctttcga
     4201 aaagaccaga gggccggaaa cgccagaacc gagtccccaa ggcgaaatga ggcacgcaca
     4261 atcagaatct tcttaataga ttccttgcgc aaatccggcg cggtcggggc ggtcgacgcc
     4321 gttatcctgc tgaggatcca aagcagtaac ccgcgaaacc gccgattgag tgagtagcgg
     4381 aaaactggt
//
LOCUS       DASULZ010000125         3187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_523137, whole genome shotgun sequence.
ACCESSION   DASULZ010000125 DASULZ010000000
VERSION     DASULZ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523137"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..1358
                     /locus_tag="VFY96_02550"
     CDS             213..1358
                     /locus_tag="VFY96_02550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5605363.1"
                     /translation="MNFSIFMMPLHHPTENPSLAFDRDISLIQYADELGYDEFFIGEH
                     HSGGWETMPAPEMALAKASAHAHRIRLGTSVISVPFHHPFHIAERMAFLDHLTRGRAI
                     LGVGPCALVTDKNLFGLATEMLYPMMAESVDIIVRLLESPEPIDYDGQFWKLKQMRLQ
                     LRSYQQPRMPLAIASSGNPVSLELAGKHGMLFLSPAGKNKRNNQTKAEQWNRVEAIAA
                     KHGTHTSRDNWRLATCVYLAESREEAWREVEANIDRDKHYFFSIGLKAPYEAYPGQPA
                     HEITARSGADRRDWIIGTPDDAIAQIERMQTETGGFGGLMLTTHEWASTDRLRRSYEL
                     FARYVIPHFRGHNQGYRDEWRRVQEAAADGGFKPDPATTNNLLLKGE"
     gene            1669..2832
                     /locus_tag="VFY96_02555"
     CDS             1669..2832
                     /locus_tag="VFY96_02555"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5605364.1"
                     /translation="MWTQETYREIVRVICSGQVIDGNALEELESIISEALAVRSVLLC
                     GSGSFALELALSAGGVQPGDEIVIPTFCCSAIVPPILSIGATPVLADIGEELNITRET
                     VAAVLTKKTRAVIVPHLFGNPADIQDILELAQRRNFFVIDDAAQAFGATIGKRPVGSF
                     GNFGILSFGDEKLCPSIGGGALVSRENDIFVAKSAMPLRSASYVTTLRRCFSTLIWNC
                     SRHWFAPLTKLRGSHSDPTVIPACYGREAMPNLYAAVAVTLVERLAEHIAARRERVRA
                     YQQLLGHNDSLELIPHRLGSACVTQVVRVLPRKRSRDIAMDVVNALAAEKYAIRGSYV
                     PLHLIPGLSACVWDRLPHADRIWSDLIELPCEPSVGFDDVTQIATIVAAVASS"
     gene            3000..>3187
                     /locus_tag="VFY96_02560"
     CDS             3000..>3187
                     /locus_tag="VFY96_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605365.1"
                     /translation="MASTHKHRAANSRGQELRAVGQGLESLDVEDFDLTTDVEGYFAL
                     ATRRVSTPTSKTAVRKVVR"
BASE COUNT          687 a          893 c          894 g          713 t
ORIGIN      
        1 atcagctact tcaccttgtc aatctaagcc ctaccggaaa agccgactgg ggcggcgggt
       61 tatatcactg agcagcggcg gcttatctcc gctaaagttc acgaacatct atcggttact
      121 tgcatgtgtc atttcgctgg atcttgcctg cgcggtttct cttgctcgcc gtaccgtttt
      181 cgcagtagag attgaatgag aagaggccga ttatgaattt ttcgattttc atgatgccgc
      241 tgcaccatcc gacggaaaat ccgtcgctgg catttgatcg cgacatttct ttgatccagt
      301 acgctgacga gctcggctac gacgagtttt ttatcggcga gcatcactct ggcggttggg
      361 aaaccatgcc ggcgccggag atggcgctgg ccaaagcgtc agcgcatgcg catcgcatcc
      421 gtctcggcac ttcggtgatc agcgtgccgt ttcatcaccc gtttcatatc gccgaacgga
      481 tggcgtttct cgatcatctg actcgcggcc gcgccattct cggagtcggc ccctgcgcgt
      541 tggtcaccga caagaatctt tttggtctcg cgaccgagat gctctatccg atgatggcgg
      601 agtcggtgga tattatcgtg cgcttactcg agtcgcccga gccgatcgat tacgacggcc
      661 aattctggaa actgaagcaa atgcgcctgc aactccgttc ctatcagcag ccgcgcatgc
      721 cgctcgccat cgcgtcgtcg ggaaatccgg tgagcctcga gctcgccggt aagcacggca
      781 tgttgttcct ctcaccggcg ggcaagaata agcggaataa tcaaaccaag gcagagcagt
      841 ggaacagagt cgaagcgatc gccgcaaagc atggtacgca cacatcgcgc gacaactggc
      901 ggcttgcgac ctgcgtctat ctcgccgaaa gccgcgagga agcatggcgc gaggtcgagg
      961 ctaacatcga tcgcgataag cattatttct tctcaatcgg attgaaagcg ccttacgaag
     1021 cgtatcccgg gcaacctgcg catgaaatca cggctcgatc gggcgccgat cggcgcgact
     1081 ggatcatcgg gacgcctgat gacgccattg ctcaaatcga gcgcatgcag acggagaccg
     1141 gcggtttcgg tggactcatg ttgaccacgc acgagtgggc gagcacagac aggctccgcc
     1201 gctcgtatga gctttttgca cgctatgtga taccgcactt tcgtggacac aaccaaggct
     1261 atcgcgatga atggcggcgc gtacaggagg ccgctgccga tggcggattt aagccggacc
     1321 cggctactac aaacaatttg ttgctgaagg gcgagtgacg aacactttct tggatcaacc
     1381 cttctttcac gccactgttc ctcaactcgc gaatgcacat accatcagac tgtgcgacac
     1441 gcccatgccc gcgcgggtgt ctctcgatac gcgcgtcgcg ctactcggga agccggttta
     1501 tccgaccatc ctgagtaggc agcgcgcagc gctgccgtat cgaagaacga gcgagagatc
     1561 ttgcactcgc agcaacatac gaagaaagat ttctcgccgg agtctaccct gcctctcaca
     1621 ccggacgcta aggaaaatag agcttccgcg actcctactc gccgtggagt gtggactcag
     1681 gaaacgtatc gcgaaattgt tcgcgtcatc tgctccggtc aagtcatcga tgggaatgcg
     1741 ctcgaagagc ttgaatctat aatcagcgaa gcgctcgcag ttcgaagcgt actcctttgc
     1801 ggctccggga gttttgcatt ggaactcgcg ctcagcgccg ggggtgtgca acccggcgat
     1861 gagatcgtga ttccaacttt ttgttgcagc gcgattgtgc cgccgattct atcgattggc
     1921 gcgacaccgg tgctcgccga catcggcgag gagttgaata tcacccgtga aaccgtcgcc
     1981 gcggtgctaa caaaaaaaac tcgcgccgtt atcgtgccgc atttgttcgg caacccggca
     2041 gacatacagg acatcctcga gctcgcgcag cgccgaaatt ttttcgtcat cgatgatgcc
     2101 gcgcaagctt tcggtgcgac cataggcaag cggccggtgg gaagcttcgg gaatttcggc
     2161 atcttgagct tcggcgacga aaaactttgt ccgagtatcg gtggtggggc gctggtatca
     2221 cgcgagaacg acattttcgt tgcgaaatcg gcaatgccat taaggtcggc ttcttacgtc
     2281 acaaccctgc gacgctgttt ctcaacgctg atctggaatt gctcacggca ctggttcgcg
     2341 cccctgacaa aactccgcgg cagccattcc gatccaacgg tgatacctgc atgctacgga
     2401 cgagaggcga tgcccaacct ctacgctgca gtggcggtca cactcgtaga gcgattggct
     2461 gagcacatcg ccgcccggcg cgagcgcgtg cgcgcctatc aacaactcct ggggcacaac
     2521 gattccttag agctgattcc tcataggtta gggtcggcgt gcgtaacgca agtcgtacgg
     2581 gttttgccca ggaagcgcag tcgggacatt gcaatggacg tagtgaacgc gctcgctgca
     2641 gaaaaatacg caattcgagg cagctatgtg cctttgcatt tgattccggg attgagcgct
     2701 tgcgtttggg atcggctgcc ccacgctgat cgtatttggt cggatctgat cgagcttccc
     2761 tgcgagccgt ctgttggctt tgatgatgtt acgcaaatcg ccacgatcgt cgccgcagtt
     2821 gctagcagtt aattccctga ctccgattgt gatctgaagg acatctatgt cattggctgg
     2881 cccgcctaga aacggaaaag ctgcgcgcga ttgccaatga gcggaaagaa ttccgctgga
     2941 ggttaaattt ccaaccggcc tgaattttgc cctgtgcaaa cgatatgaaa tccgaacgaa
     3001 tggcttcaac ccacaaacat cgagcggcga actcgagagg gcaggaattg cgcgctgtcg
     3061 gtcagggttt ggagtctctc gatgtcgaag atttcgatct aactacggat gttgaaggct
     3121 atttcgcctt ggctacgcgc cgtgtttcca ctccaacatc aaagactgcg gtcagaaaag
     3181 ttgttcg
//
LOCUS       DASULZ010000126         2869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_16077, whole genome shotgun sequence.
ACCESSION   DASULZ010000126 DASULZ010000000
VERSION     DASULZ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16077"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VFY96_02565"
     CDS             complement(<1..499)
                     /locus_tag="VFY96_02565"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5605366.1"
                     /translation="MPSTSVLIIGAGLAGLAAAYQLERSGRSARIVEARDRVGGRVWT
                     LRQGLGGMHAEAGGELIDDDQEEIRKLLREFKLPEARILRHGFSQYRLGRNGHRQLRA
                     SSTGWRLTAQALEPLLRLYRLNGEEWTGPIAAGIAKESVAEWLSPNNKTLFPRAARRD
                     AANTAE"
     gene            complement(702..1871)
                     /locus_tag="VFY96_02570"
     CDS             complement(702..1871)
                     /locus_tag="VFY96_02570"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017309118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HEX5605367.1"
                     /translation="MKANCWYGKGDVQVVDVSEPEILNSRDAVIKVTSTAICGSDLHL
                     YNGFVPTMEKGDILGHEFMGEVVEVGNEVHNLKAGDRVVASFPISCGKCFFCQKELFS
                     LCENSNPNAWMAQKLFGQSPAGIYGYTHMLGGFAGGQAEYARVPFADVGTVKIPDGLS
                     DEQVLFLSDIFPTGYMAAENCNIQPGDTIAVWGCGPVGQFAMKSAYLLGAERVIGIDR
                     FPYRLRIAREKVGAEVLNYEECDVYDSLMDLTGGLGPDACIDAVGMEAHVPGVIGAYD
                     RVKQSMMLESDRPHALRQAIMACRKGGTVSIPGVYGGFDDKIPLGSLMNKALTVKTGQ
                     THVQHYFAPLLERIQDGDIDPSFIITHRLPLDQAPHGFEMFLNKQDDCLKVVLKP"
     gene            complement(1886..>2869)
                     /locus_tag="VFY96_02575"
     CDS             complement(1886..>2869)
                     /locus_tag="VFY96_02575"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5605368.1"
                     /translation="LTSAARRAWDQKQLGILIDGNERTVARGLGWFSIGLGLAEVLAP
                     RTLERFLGIRHHGFLLRVMGLREIASGVGILTGRRPSDWLWARVGGDVIDIASLGMAL
                     SSDTAKPANIGLACVAVAGVTVVDMHCAQELSRTNDAALSARTIEVRKSIQINCSPDA
                     VYKFWRNFQNLPKFMSNVESVETLSDKRSHWCVRGPAGKRIEWDAEITDEQENHLIAW
                     RSLEGAQVQNYGTVRFEAAPGGRGTYVQVHLQYSPPAGIFGARVAKLFGREPRQQIHE
                     DLHHLKQIMETGEIITTEGQPAGRARGTSWKYDQAVRRQVDSRSAYQTSAA"
BASE COUNT          573 a          871 c          777 g          648 t
ORIGIN      
        1 tctcggcagt attggcagca tcccttcgag cggcgcgcgg gaaaagagtt ttgttgttgg
       61 ggctcagcca ctccgcaacg ctttcttttg caatgcccgc agcaatcggc ccggtccatt
      121 cttcgccatt caaccggtaa aggcgtaaca ggggctccag cgcttgcgcc gtgagacgcc
      181 aaccggtgga cgaggcgcgg agctgccggt ggccattcct gccaaggcga tattgagaaa
      241 acccgtggcg caagattcgt gcttcaggga gcttaaattc gcgcagcagt tttctgatct
      301 cttcctggtc atcatcgatc agctccccgc cggcttccgc atgcatcccg ccgaggccct
      361 ggcgtaacgt ccacacccga ccgccaactc ggtcacgtgc ttcgacgatc ctcgcgctcc
      421 ggccacttcg ttcgagttgg taggcagcag cgagccctgc gaggccggcg ccgatgatca
      481 acaccgatgt gctgggcacg gtgttaagct tttcatatgt tgcggttggt gtgaaatccg
      541 gcctagaagc tgccggtgag caagctcgct cttcccggat ccgggctcag cttttcggcg
      601 atgcatctct gcgagagttt tcagcatctg gtttttccga ggtctgtctc aagcattggt
      661 gctccgttct tgccgccacg ttcgcgtaca acgccctttc tttacggctt gaggacgacc
      721 ttcaagcagt catcctgctt gttcaaaaac atctcgaagc cgtggggcgc ctggtcgagc
      781 ggcagccggt gagtaatgat gaagctcgga tcgatatcgc cgtcctgaat cctttccagc
      841 agtggcgcaa agtagtgctg cacgtgtgtt tgtccagtct tgaccgtcag tgccttattc
      901 attaaagaac cgagcggaat tttatcgtcg aaaccgccgt acacacccgg aatcgatacc
      961 gtgccgcctt tgcggcacgc catgatcgct tgccgtaatg cgtgtggtct atcgctttcc
     1021 aacatcatcg attgcttgac ccgatcgtag gcgcctatga ctcctggcac atgtgcttcc
     1081 atcccgaccg cgtcgatgca ggcatcgggg cccagcccgc cggtcaaatc catgagggaa
     1141 tcgtacacgt cacattcctc gtaattcagc acctccgctc cgactttttc acgtgcgatt
     1201 cgcaagcgat agggaaatcg atcaatgccg atgacgcgct cggctccgag caagtaggca
     1261 ctcttcatgg cgaattgtcc gaccgggccg caaccccaaa cggcgatcgt atcaccgggc
     1321 tgaatgttgc agttttccgc ggccatataa ccggtcggaa aaatatccga caagaataag
     1381 acctgttcgt cgctcaagcc gtcgggaatc tttaccgtgc ccacatcggc aaagggcaca
     1441 cgagcgtatt ccgcctgtcc gccggcgaag cccccgagca tgtgggtgta gccataaatt
     1501 cccgcggggg attggccgaa aagcttttgc gccatccaag cgttggggtt ggaattctca
     1561 cacagcgaaa aaagctcctt ttgacagaaa aaacatttcc cgcacgagat cgggaacgaa
     1621 gcgaccaccc gatcgcctgc cttcaggttg tgaacttcgt tgccgacctc aacgacttcg
     1681 cccataaact cgtgaccgag aatatcgccc ttttccatgg tgggcacgaa gccgttgtaa
     1741 agatgcaaat ctgagccgca gatcgcggta gacgtgacct tgatcacggc gtcgcgacta
     1801 ttgaggatct ccggttccga gacgtcgacg acttgaacgt ctcctttgcc ataccagcag
     1861 tttgccttca tgggttctcc ttttctcagg cagcggaagt ttggtaggcc gatctcgaat
     1921 cgacctgccg tcggacggcc tggtcatatt tccaggaggt tccgcgggcg cgaccggcgg
     1981 gctgcccctc ggtcgtgatt atttctccgg tctccatgat ctgtttgagg tggtgcaaat
     2041 cctcatggat ctgctgccgc ggctcacgcc cgaaaagctt cgccacccta gcgccgaaaa
     2101 tgcccgcggg cgggctgtat tgcagatgga cttgaacata ggtgccgcgt ccgccgggag
     2161 ccgcctcgaa ccgcacggtg ccgtagttct ggacctgcgc tccttcaagc gaacgccagg
     2221 cgatcaagtg attctcttgc tcgtcggtaa tttcagcgtc ccactcgatg cgcttgccgg
     2281 cagggcctct gacgcaccag tgcgagcgct tatcgctcag tgtctcaacc gactcgacat
     2341 tgctcataaa tttgggcagg ttttgaaagt tacgccagaa tttgtaaacc gcatccggtg
     2401 aacagttgat ctggatcgac tttctcacct cgatcgtgcg ggcgctgagc gcagcatcat
     2461 tggttcggct aagttcttga gcacagtgca tatctacaac ggtgactccg gcgaccgcca
     2521 cgcaagccag tccgatattg gccggtttcg ccgtatcaga actaagcgcc ataccgaggc
     2581 tggcgatatc gatcacatcg ccgcccacgc gcgcccatag ccagtcggac ggacggcgcc
     2641 cggtcaagat tcccacgccg gaagcaattt cacgcagtcc catgacccgc agtaaaaatc
     2701 catgatgcct gatgccgagg aatcgttcga gcgttcttgg cgccaagact tccgctaagc
     2761 caaggccgat gctaaaccaa ccaagcccgc gcgccacggt tctttcgttt ccatcgatca
     2821 gaatgcctag ttgtttttgg tcccacgctc tgcgcgccgc tgatgtcag
//
LOCUS       DASULZ010000127         2100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_523413, whole genome shotgun sequence.
ACCESSION   DASULZ010000127 DASULZ010000000
VERSION     DASULZ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523413"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VFY96_02580"
     CDS             <1..568
                     /locus_tag="VFY96_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605369.1"
                     /translation="KDFLVSSGGLILSRNKESYQKASMQAFLDGAQLPGLAMMQLAIS
                     LRDIFAADNYIKSRVEQVRYLWQRLKDRIPVVSPPGGHAVFIDVKAFLPDLQSAEFRS
                     EALGAFLYRYAGIRVTKGPPPAPSQAARGMELLRLAIPARKYLNAHLDDVAEALCYAY
                     SRRNEITGLKRSEDPVRSKYDPGYFQPL"
     gene            565..>2100
                     /locus_tag="VFY96_02585"
     CDS             565..>2100
                     /locus_tag="VFY96_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5605370.1"
                     /translation="MNDRAVQSVASTPYRHIGKIFFQRVAELGERPFIRFQESTGPVD
                     ISWREFGAMVRNILLALYGLGLMPGDAIAIIGENSLERVCADLATLAGGFPNVVISPA
                     MSGGMLLKVLDHSRCRAAFVQSEMDASRLLNLKRELPALEHLFMMNGTAAPLPDTVPF
                     LELMEQGQAFDAARLEEILESVHAQDLATIMYTSGSTGEPKGVMRTQDNLLSNITNGG
                     EVIISKPDELYVIVLSLNHLLGRFGFLKSAITGRTTAIVESTELNLDLDVIQGLSGTA
                     MALVPRVMERIWKDLLDRGDHRQLWETIEALDQKKTAGRLAEADSKKLEECITVLREA
                     ARKALGGRMKYISYSGAAMPPRIMRFFELIGIPLLGSYGSTECGGVTLCGIGENRPGN
                     LGKPFPNVEVRIAADAEILVRGPTVSPGYFLNPEATREVLDTDGWFHTGDLGALEPDG
                     SLRIIGRKKDVFYCADGSNIYPAFIELQLENETFIRQAVLLGDRRPFISALIVPERQR
                     IADA"
BASE COUNT          452 a          557 c          610 g          481 t
ORIGIN      
        1 caaagatttt ttagtgtctt cgggcggact tatcttgagt cggaacaaag agagctatca
       61 gaaagcttcg atgcaggctt ttctcgacgg cgcgcagctg ccgggcctag cgatgatgca
      121 actagcgatt tcgctgcgcg atatttttgc cgcggacaat tatatcaaaa gccgcgtcga
      181 acaagtccgt tatctgtggc aacgattaaa ggatcgtatt cccgtcgtga gtcccccggg
      241 cgggcatgct gtgtttatag acgtcaaagc atttttgccg gatttgcaat ccgctgaatt
      301 ccgcagtgaa gctttggggg cgtttttgta tcgctatgcg gggatcaggg tgaccaaagg
      361 cccgccgccg gcgccgagcc aagccgcgcg cggtatggaa ctcctccggc tggccatacc
      421 cgcgcgtaaa tatcttaacg cgcatctgga tgacgtggcg gaggcgcttt gctacgccta
      481 ctcaaggcgc aatgaaatta ccgggctgaa acgcagcgaa gacccggtgc gctcaaaata
      541 cgatccgggc tactttcaac cgctatgaac gaccgcgccg tccaatccgt tgcttcaact
      601 ccataccgcc atatcggaaa aattttcttt cagcgtgttg ccgagcttgg cgagcgcccg
      661 ttcatcagat tccaagagag caccggaccc gtggacattt cctggcgtga gttcggcgcg
      721 atggtcagaa atattctctt ggccttgtat ggtctgggtc tgatgccggg agacgccatt
      781 gcgattatcg gtgaaaacag cctcgagcgg gtctgtgctg acttggcgac attggccggt
      841 ggatttccca atgtggtgat ttcccccgcg atgtcgggtg gcatgctgct caaagtgctc
      901 gaccattcac gctgccgcgc agcgtttgtg caaagcgaaa tggacgcaag ccgactgctc
      961 aatttgaagc gagagttgcc cgcgttggag caccttttca tgatgaacgg aaccgccgca
     1021 ccgttgcccg atacggtgcc gtttctggag ctgatggaac aaggccaagc gttcgatgcc
     1081 gcaaggctcg aagagattct cgaatcggtg catgcgcaag atttggcaac catcatgtat
     1141 acatccggtt ccaccggcga accgaagggc gtcatgcgca cccaagataa tcttttatcg
     1201 aacattacta acggcggcga ggtgatcatc agcaagccgg atgagttgta cgtaattgtt
     1261 ttgagcctga atcatctgct cggcagattt ggtttcctaa agagcgctat tacgggcagg
     1321 acgacggcga tcgtcgaatc gaccgaactg aatctcgacc tcgatgtcat tcagggcttg
     1381 tcgggaaccg cgatggctct ggtaccccgg gtaatggagc gaatctggaa agacctcctc
     1441 gatcgaggtg atcatcgaca gctctgggag acgatcgaag ccctcgatca aaaaaagaca
     1501 gctggacgcc tcgccgaagc ggacagcaag aaattggaag agtgtataac cgttctgcgg
     1561 gaggccgcgc gcaaagcgct tggcgggcgc atgaagtata tctcgtatag cggagcggcc
     1621 atgccgccgc gaattatgcg cttctttgaa ttgatcggca tcccgctgtt gggatcctac
     1681 ggctcgaccg agtgcggtgg agtgaccctt tgcggcattg gcgagaatcg cccgggtaat
     1741 ttaggcaaac cctttcccaa cgttgaggtt cgcattgccg cggatgcgga aatcctggtg
     1801 cgcggtccga cggtctcgcc gggttatttc ctgaaccccg aggccacccg cgaagtgttg
     1861 gataccgacg gctggtttca taccggcgat ctcggcgcgt tggagcccga cggctcgctg
     1921 cggattatcg gcagaaaaaa agacgttttc tattgcgcgg acggatcgaa catttatccc
     1981 gcttttatcg agttgcagtt ggaaaatgag acgttcattc gtcaagcggt cctgctcggc
     2041 gatcgtcgtc cattcatttc ggcattgatc gttcctgagc gccagcgcat cgccgatgct
//
LOCUS       DASULZ010000128         7204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_218222, whole genome shotgun sequence.
ACCESSION   DASULZ010000128 DASULZ010000000
VERSION     DASULZ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7204
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218222"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2059)
                     /locus_tag="VFY96_02590"
     CDS             complement(<1..2059)
                     /locus_tag="VFY96_02590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX5605371.1"
                     /translation="MTQSKLRFLSISLVVLLVVTSTGLVFGAWYLKQLENTVTEKFEG
                     RKWVFPSKVYSDSYLLYVGAPLRSENLIEKLRRLGYYATSGELRAKGDYRVLRSSTIE
                     IFLHDFAFPTEQFKGVPVRLSLQGTLLARIENIATGQEMFSLELEPELVTGLYERIWQ
                     ERRLVKLTDVPPLLVKAILAVEDERFYRHHGVDPLGILRAMWVNLRTLSYQQGGSTLT
                     QQLVKNFFLSDERTMSRKIPEAMMALIAERKYSKDTILENYLNEIYLGQKGSQGIFGV
                     WEAAQFYFAKPLSDLTTGECALLAGLIRAPNRLSPYRSYEAATRRRNVVLVKLLDDRI
                     ITRKEYDMALREKLPQRALVKVTNDAPFYVDYLRRELEENYSNAVLTKEGLRIFSSLD
                     LQLQKIAERALVEGLNKLEAAHPALRRKGEESLEGAIVVLRPQTGEIKAMVGGRNYSK
                     SQFNRVFQAKRQPGSVFKPFVYLAALMYGGQSGIKYTPDTIINDSQFTWSYDGRDWQP
                     SNYRNEYFGAVTLRRALESSLNSATSRVAQDVGIRRVRDMAHRLGIQSSLPAVPSLAL
                     GAAEVTPLEVAVAYSTLANGGVRPRPLAVRNVIDQNTKVLEKRDVRSEQVISPQLAFV
                     MNDLLRGVLDRGTGAMARRWGFTRPAAGKTGTTNDYKDAWFVGYTPDLLTVVWVGFDG
                     PSKL"
     gene            complement(2162..3661)
                     /locus_tag="VFY96_02595"
     CDS             complement(2162..3661)
                     /locus_tag="VFY96_02595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEX5605372.1"
                     /translation="MKLKEIWGGGVGVNGAVLIALVSLAIGLGISGSLDWLAPSRAVN
                     LLGDAGNSEVRTSAGLPDFVSLAKKMRPVVVNISTTQVSEGRGGASPEFGSPFGEEDP
                     FNDFWRRFFGGPMPRGPQRQRSLGSGFVIDGDGSILTNNHVVENASKIVVKLSDDQEY
                     EAKVVGRDQKTDIAIIRIEGKPNLPAASLGDSDTLEVGEWVVAIGNPFGLDSTVTSGI
                     VSAKGRHIGQGPYDNFIQTDASINPGNSGGPLINLRGEVIGINTAIFSRTGGNIGIGF
                     AIPINLVKELLPQLRGKGKVTRGYLGVLIQKVTPEIAESLGMEKANGALVANVSKDGP
                     ADKAGVKVGDVIVEFDGKEVKDSGDLPILVARTAVDKKARLKVLRDKKEVALTVAVGE
                     LKDEEVVATAPEKGELGLTVQRLTPQMAESLGLEKSEGVVVTAVEPGSAADEAGIRRG
                     DIILEVDRKTVRNLDEYKKAIAGARKGRGVLFLVRRGDNTLFLALKPQR"
     gene            complement(3672..4109)
                     /locus_tag="VFY96_02600"
     CDS             complement(3672..4109)
                     /locus_tag="VFY96_02600"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HEX5605373.1"
                     /translation="MADEESKQAGKGFTVQDRRRFSPDTGEAREEAPEQTPQQAETQP
                     QSSEERRESSGQDASGEALPEINFSTFVIGLSTQALMHLGEIANPMSGKVEIDVPTAK
                     QMIDILGLLKDKTRGNLNASEDRLMEDILFDLRMKYVEAVKKR"
     gene            complement(4299..5312)
                     /locus_tag="VFY96_02605"
     CDS             complement(4299..5312)
                     /locus_tag="VFY96_02605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX5605374.1"
                     /translation="MKSLPNTARVATQRNVCVLFPGALGDFICFAPTLQLLAEAAHVD
                     VYARSEFADLVPKGITVHSLEQSAIRKLFTAEVETDEEAQCFFGNYSTVYSWFGSQQA
                     DFVRRITAASFGRAQIFPFRPAGARVHQVDHYLSCLRVGGTLNRQPVIELRAEAIRWR
                     VDFWRKQKLNGHPVLVIGAGSGAREKNWPEEFLVRVVKWWRTATRGNVVLPVGPVEQE
                     RGGIERLSRYCVTVSGLGLAELAALIAGSDLYLGNDSGVSHLAAAVAVRTVALFGPSD
                     IEQWAPRGTRVTIVRHSVHCSPCETPAMKNCPHRSCLTALLPDEVIQALAQLPEVLTL
                     TRG"
     gene            5406..6350
                     /gene="cysK"
                     /locus_tag="VFY96_02610"
     CDS             5406..6350
                     /gene="cysK"
                     /locus_tag="VFY96_02610"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009710785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX5605375.1"
                     /translation="MNGARSVLDLIGSTPVVKLKALPGKNDADVWAKLESFNPGGSVK
                     DRICLNMIETAERQGKLKPGETIVEPTSGNTGIGLALIAAVKGYRLILTMPDTMSEER
                     RSLLCAYGARLILTPDTKGMGGAIHKAEELLEQNTDYFMPQQFNNPANPEIHRRTTAV
                     ELLKQFKRIDAFVAGVGTGGTITGVGEVLKQKMKGVRICAVEPGASPVISGGEPGFHK
                     IQGIGAGFIPAVLNRQILDEVITVTDDDATSYTRRLAAEEGLLVGISSGAACCAALKV
                     AKTLGAGHVVVTIFADKGEHYLSTDLFSDKKWTRNLND"
     gene            6326..7138
                     /gene="larE"
                     /locus_tag="VFY96_02615"
     CDS             6326..7138
                     /gene="larE"
                     /locus_tag="VFY96_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015207283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEX5605376.1"
                     /translation="MDPKLERLTKILREQQRILVAFSAGVDSTFLLKAAYLALGDGAV
                     ALTASSPTAPPGELEAAKAFAADLGCRHIVLDSHELANPLFAKNPVNRCFFCKDELYR
                     ICREEADKLGISTIVDGTNLDDLHDHRPGLQAANEWGVRHPLVEAEMTKDDIRRYSQA
                     LALPTWDKPSSPCLSSRFPYGTEIDLEKLRKVGVCELFLKQLHFREFRVRYHGDLARI
                     ELAPNEIERMLDSSIRAAVVSKFKEVGFNYVSVDLQGFRSGSLNEVLRNKSV"
BASE COUNT         1589 a         2123 c         1896 g         1596 t
ORIGIN      
        1 ttaacttact tggaccgtca aaacctaccc aaacgactgt cagcaagtcc ggcgtgtaac
       61 cgacaaacca cgcatccttg taatcattgg tcgtgccggt ttttccggct gccggacgcg
      121 taaagcccca gcgtcgcgcc atcgctcctg tccctcggtc gagcactcct ctgagaaggt
      181 cattcatcac aaaagcgagc tgtggggaaa tcacctgctc gcttctaacg tcgcgcttct
      241 ctaaaacctt ggtgttttga tcgatcacat tgcgaaccgc caaagggcgc ggccgtacgc
      301 caccgttggc cagagtcgaa taggcgacgg ccacttcgag cggcgtcacc tccgctgcgc
      361 ctagagcaag cgacggcact gccggtaaag agctttggat gccaagacga tgcgccatgt
      421 cacgcacgcg ccggatgccc acgtcctgcg ccacccggct ggtcgccgag ttgagcgaac
      481 tttccaaggc gcggcgaagg gtcaccgccc caaaatattc gttgcgataa ttgctcggct
      541 gccagtccct gccgtcatag ctccaggtga attgactgtc gttgataatc gtatcaggcg
      601 tgtatttaat tccgctttgt ccaccataca taagcgcggc gagataaacg aatggcttga
      661 agaccgagcc cggctggcgc tttgcctgga acacgcgatt gaattgcgac ttggaatagt
      721 tccgtccgcc caccatcgcc ttgatctcgc cggtttgcgg ccgcagcacc acaatcgctc
      781 cttccaggct ttcctcacct ttccgacgca gcgccggatg cgcggcttcg agcttattca
      841 agccttctac cagagcgcgc tcggcgatct tttgcagctg caaatcaaga ctgctgaaaa
      901 ttcttagccc ctctttcgta agaaccgcgt tggaatagtt ttcttcaagc tcgcgacgca
      961 gatagtcgac ataaaaaggc gcatcattgg taaccttgac gagcgcccgc tgcgggagct
     1021 tttcccggag cgccatgtcg tactccttgc gcgtgataat acgatcatcg aggagcttga
     1081 ccaacacgac gttgcggcgc cgggtcgccg cctcataact tcggtaggga gacagccgat
     1141 tgggcgcgcg aatcaatcct gcgagcagcg cgcattcacc ggtcgtcaaa tcggacagcg
     1201 gcttggcgaa gtagaattgt gccgcttccc agaccccgaa aattccctgt gagccttttt
     1261 gtccaaggta gatttcgttc aggtaatttt ccaggatggt gtcctttgag tacttccgct
     1321 ccgcgatcag cgccatcatc gcttcgggaa tttttcggct catagtgcgc tcatcggaga
     1381 gaaaaaagtt cttcaccaac tgctgcgtta aagtgctccc gccctgttga taactcaggg
     1441 tacgcaggtt gacccacatc gcgcgcaaaa ttccgagcgg atcgacgccg tggtgccgat
     1501 agaaacgttc gtcttccacc gccagaatcg ctttcaccag cagcggcggc acatccgtca
     1561 gtttgaccag cctgcgttcc tgccagatcc gttcgtagag cccggtgaca agctcgggtt
     1621 cgagctcgag cgaaaacatt tcctgtccgg tagcaatgtt ttcgatgcgc gccaacaaag
     1681 tgccctgcag cgaaagccgg accggcacgc ctttgaattg ctcggtagga aaagcaaaat
     1741 cgtgcagaaa aatttcgatc gtgctggagc gcaaaacgcg ataatcgccc ttggcgcgta
     1801 attcgccgct tgtggcgtaa taacccagtc ggcgcagctt ctcgattaaa ttttcagagc
     1861 gcagcggagc accgacatac aagagatagc tgtcggaata aacctttgac ggaaaaaccc
     1921 actttctgcc ctcgaacttt tccgtcaccg tattttccaa ctgcttcaag taccacgcgc
     1981 cgaagaccag cccggtggag gtgacaacta agaggacgac aagggaaata ctaagaaatc
     2041 ggagcttgga ttgtgtcaaa cgtgcgcctc gggatagcta attttcgcgc agaaccgaca
     2101 caaaagcaac gagaacggat ctaaatccgc tctcgtcaca tgaagtaaat cgtcgaaaag
     2161 atcagcgttg aggcttaagc gccaggaaga gtgtattatc gccgcgcctg accaggaaca
     2221 gcacgccacg tcctttgcgc gcaccggcga tggctttttt gtattcgtcg agattacgaa
     2281 ccgtcttgcg atcgacttct aaaatgatgt cgccccggcg aatgcccgcc tcatcggcgg
     2341 cgctgccagg ctccacagcg gtgacgacga caccttctga tttttccaat cccaagcttt
     2401 cggccatttg cggcgtgaga cgctgaacgg tcaatcccaa ttcgcccttt tccggcgccg
     2461 tcgcgaccac ttcctcatcc ttgagttcgc caacggcaac ggtgagcgcg acttcttttt
     2521 tgtcacgcag caccttcagc cgcgcttttt tgtctacggc ggtgcgggcc accaggatcg
     2581 gcagatcgcc cgaatctttg acttccttgc cgtcgaactc gacaatcacg tcaccgacct
     2641 tcacacccgc tttgtctgcc ggtccatcct tcgaaacatt ggcaaccagc gcgccattgg
     2701 ctttctccat gcccagactc tcggctattt ctggcgtcac tttttggatc agcacgccga
     2761 gatagccgcg cgtcactttg cctttgccgc gcagctgcgg cagcaattct ttgacgaggt
     2821 tgatggggat cgcgaagcca atgccgatat tgccgccggt gcggctgaaa atagcagtat
     2881 taatgccgat gacttcgccg cgtaaattga tcagcggtcc acccgaattc cccgggttga
     2941 tcgacgcatc ggtctgaatg aaattatcat atggcccctg accgatgtgc cggcccttgg
     3001 cgctgacgat acccgatgtc acggtgctgt cgagtccgaa cggattaccg atggcaacga
     3061 cccattcgcc aacttcgagc gtatccgagt cgcccaggct ggcggcggga aggttcggct
     3121 tgccttcgat tcttatgata gcgatatcgg ttttctgatc gcgcccgaca actttggcct
     3181 cgtattcttg atcatcggag agcttgacga cgatcttgct ggcattctcg actacgtggt
     3241 tgttggtgag tattgagccg tcaccgtcga taacaaatcc ggagcccaaa cttcgctgcc
     3301 gctgcggacc gcgcggcatc ggcccgccaa aaaaacggcg ccagaaatca ttgaaaggat
     3361 cctcctcgcc gaacgggctg ccgaactccg gcgatgcacc accgcgcccc tcgctgacct
     3421 gagtcgtaga aatgttgacc accacgggcc gcattttctt cgccaagctg acaaagtccg
     3481 ggaggccggc gcttgttcgg acttcgctat tgccggcatc tcccaaaagg ttgactgccc
     3541 gactaggcgc caaccaatcg aggctaccac tgatcccgag tcctatggcg agcgaaacca
     3601 aggcgatcaa cacggccccg ttcacgccca cgccaccacc ccagatctct ttcagtttca
     3661 tcgaatggct cctatcgttt tttgaccgcc tcgacgtatt tcatgcgcaa atcgaacaag
     3721 atgtcttcca tcagccgatc ttcgctcgca ttaagatttc cccgcgtttt gtctttcagc
     3781 aaccccagta tatcgatcat ttgcttcgcc gtgggcacat cgatttcaac tttgccgctc
     3841 atcgggttgg caatctcacc gagatgcatg agcgcttgcg tgctcaaacc gataacaaag
     3901 gtcgaaaagt taatctccgg caacgcctca ccgctcgcgt cctgaccgct tgattcgcgc
     3961 cgctcttcgc tggattgcgg ctgagtctcg gcttgctgcg gagtttgctc tggagcttcc
     4021 tcccgcgctt cacccgtatc cggtgaaaaa cgtcgccgat cctgcacggt aaaccctttg
     4081 cccgcctgtt tcgattcttc atcagccatg gttcaactcc ggagtgacac gaaaaaaaac
     4141 ctggatatcg tgcgactctc aagccgtccg agtaccaggt gtcttatatc ctaattcaat
     4201 gcttcgtaat tatctgccgg tgacaaatac gagcaaccta aaccgctgct tttgctttcg
     4261 ttttgccaat ctttctcgcc tcaaaatgta atccctggct accctcgtgt caaggtaagc
     4321 acctccggca gctgtgccaa agcttggatc acttcatcgg gtaacagcgc tgtcaggcaa
     4381 gagcggtgcg ggcagttttt catcgcggga gtttcgcagg gcgaacaatg gacagaatgt
     4441 cgcacaatgg taactctcgt gccgcgcggc gcccattgtt caatatccga gggaccgaac
     4501 aaagccaccg ttctaacggc aactgcggcc gcaagatgag acacgccgct atcattgcca
     4561 agatatagat cactaccagc gatgagcgcc gccagctccg ctaaacccaa tccagagacc
     4621 gtgacacagt aacgcgacag ccgctcgatt ccgccacgtt cttgttcgac cgggccgacg
     4681 ggcaaaacga catttcctcg cgttgcggtc cgccaccact tcaccacacg aactaaaaat
     4741 tcctccggcc aattcttttc gcgcgcgccg cttcccgctc cgatcacaag cacgggatgc
     4801 ccattgagct tctgttttct ccagaaatcc acgcgccacc ggatagcctc agcgcggagc
     4861 tcaatcaccg gttgccggtt tagtgttcca ccgacgcgaa gacaactcaa ataatgatcc
     4921 acctgatgaa cgcgtgcccc cgcggggcga aacggaaaaa tttgtgctct gccgaatgac
     4981 gctgcagtaa tccgccggac gaaatcggct tgctggctcc caaaccacga ataaaccgtc
     5041 gagtagttgc cgaagaagca ctgcgcttct tcgtcagtct caacttctgc ggtgaagagc
     5101 tttcggattg ccgattgttc gagggaatga accgtgattc ccttcggcac aagatcggcg
     5161 aactcactgc gcgcgtaaac atcgacgtgc gcggcttccg cgagcagttg aagcgtcggg
     5221 gcaaagcaga tgaaatcgcc cagcgcaccg ggaaaaagca cgcagacatt tcgctgggtc
     5281 gccacccgag cggtgttagg gagtgatttc attgactttg ctggggtgag aatatagcat
     5341 tcaccaaggc ttaaatcgaa gttctcgccc cttacgaaag acgagtgaca ttgcctgtga
     5401 caaatttgaa cggcgccaga tccgtcctgg atctgatcgg ctcgactcca gttgttaagc
     5461 tcaaggcgct ccctggaaaa aacgatgcgg acgtctgggc taaattggag tcgtttaatc
     5521 ccgggggatc ggttaaggat cgtatttgtc ttaacatgat cgagacagcc gagcgccagg
     5581 gcaagctcaa accgggagag accatcgtgg agcccaccag tggcaataca ggcattggcc
     5641 tcgcgctcat cgccgcggtg aagggttacc ggctcatact gaccatgccc gacaccatga
     5701 gtgaagagcg gcggagcctg ttgtgcgctt acggcgcgcg gctaattttg actccggaca
     5761 cgaaaggcat gggcggcgcg attcacaaag ccgaagagtt gctggagcaa aacactgact
     5821 actttatgcc gcagcagttc aataatccgg cgaatccaga aattcaccgg cgcactacgg
     5881 cggtcgaact gctgaagcaa ttcaagcgca tcgatgcgtt tgttgccggc gtcggtacgg
     5941 gcggtacgat caccggcgtc ggcgaggttc ttaagcagaa aatgaagggc gtccggatct
     6001 gtgccgtcga gcccggggca tcacctgtca tatcgggcgg agaaccgggc tttcataaaa
     6061 ttcagggcat cggcgcgggg ttcattccgg cggtattgaa ccggcaaatt ctcgatgagg
     6121 tcatcaccgt caccgatgac gatgccacga gctacacgcg tcgcttggcg gccgaggaag
     6181 gtctgctggt cgggatctct tcgggcgcgg catgttgcgc ggccctcaaa gtggccaaga
     6241 ctcttggagc cggtcacgtc gtggtcacga tcttcgcgga caaaggcgaa cactatttga
     6301 gcacggatct tttcagcgac aaaaaatgga cccgaaactt gaacgactga cgaaaatcct
     6361 gcgcgagcaa cagagaatcc tcgtggcgtt ttcagcaggc gttgactcga cttttttgct
     6421 gaaagctgcc tatttggctc tcggcgatgg ggctgtcgct ctcactgcat cctcgcctac
     6481 ggcgcctccc ggcgagctcg aagcggccaa agcctttgct gcggatctgg gttgccgtca
     6541 tatcgtgctg gactctcacg agttggcgaa tccgttattc gcgaaaaatc ccgtcaatcg
     6601 ctgctttttt tgcaaagacg aactctatcg catttgccgc gaggaagcgg ataagctcgg
     6661 catcagcacg attgtcgacg gcacaaatct cgacgatttg cacgatcacc ggcccggctt
     6721 gcaagccgcg aatgaatggg gcgtgcgcca tcctctggtc gaagcggaga tgacgaagga
     6781 tgacatccgg cgttacagcc aggcgctcgc tctaccgacc tgggacaaac cgtcgagtcc
     6841 ctgcctctct tcgcggtttc cgtacggaac tgagatcgat ctcgagaagc tgcgcaaagt
     6901 gggcgtttgc gagctctttt tgaagcaact gcactttcgg gagtttcgcg tacgttatca
     6961 cggcgatctc gcgcgcatcg agctcgcgcc gaatgaaatc gagcgcatgc ttgattcttc
     7021 gatccgggct gcggtcgtca gcaaattcaa ggaagtaggt ttcaattacg tgagcgtcga
     7081 cctgcaaggt tttcgcagcg gcagcctcaa tgaggtattg cgaaacaaat ccgtctagga
     7141 aacacgtccc tctcgtagta aaagttccgc gagccagaca tcttccggca cggtaatttt
     7201 aaaa
//
LOCUS       DASULZ010000129         2353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_16351, whole genome shotgun sequence.
ACCESSION   DASULZ010000129 DASULZ010000000
VERSION     DASULZ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16351"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..1158
                     /locus_tag="VFY96_02620"
     CDS             181..1158
                     /locus_tag="VFY96_02620"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX5605377.1"
                     /translation="MLVISEQQVRGLVDIDELIEALEKAHIQYSTDRAVMPVRLVVPL
                     PAIQGRITSMPGFLTDDKALGMKVVTYFQQNPKRHLPAILATIMMFDADTGKIIAIMD
                     GSYVTAIRTACASAMATKVLANPDSKVVGILGAGVQARAHIEALTRVRNIGAFKIYSP
                     SGVSAAKLQEEMRGRIDSAIEVVSSAEQAIRDADLVITVTTAKAPIIKADWLKPGTHI
                     NAVGSHRPDLRELDGATLARAKVVVDSRAAVMAECGDILLAIQERSIAPSAVHAEIGE
                     VLAGTKPGRTNESEITLYKSVGIAIQDVAAAHLIYNKALQQGVGLAVEI"
     gene            1212..>2353
                     /locus_tag="VFY96_02625"
     CDS             1212..>2353
                     /locus_tag="VFY96_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX5605378.1"
                     /translation="MTWDLTSYFPSFGGPEMLQFKQALREDIAALQKTAAALPQLNVD
                     SVSAWEDVLLRNEKLMRRMSHLSSYISCLASADGRNESYQREEAEIIRDRAELGKVRI
                     ELFRAFKGVTDEVFAVLFKKPSLVGAENYLHRLREEAHRAMSSDKEVLATELGVDGIQ
                     GWGQLYNTLSSKLQFEMEFPDGSRKILPISQRRSLLDHPDRRIRAAAFASGNKAWQSI
                     ADTAASALNAIAGTRLTLNRHRGVNHFLDIALFQAAITRKTLEAMFDALFANIEIPRR
                     ILRFKANLMGRQSLAWYDLGAPLDLSQESARSWDEAVAMVSAAFTRAYPALSRFFQQE
                     VIGKNWIDWEPRDGKRSGGFCTSSMLSKESRIFMTYSQALGDVLTLA"
BASE COUNT          533 a          667 c          640 g          513 t
ORIGIN      
        1 tttttgtgct ccgatgtgcg aacccacccg ccggcgcgcg ccgccgaacg ccgagtctgc
       61 tggcgcgttg agcttatcca acggcacgtc ggaaatatcg atcacatgtt tgtgttttcc
      121 catgactact ccttatccat aaggtgtgaa tcctcgcgtt ctgtgctatc tgaatgaagc
      181 atgctggtga tttcagagca acaagttcgc ggtcttgtcg acattgacga gctgatcgaa
      241 gcactggaaa aagcacacat ccaatactcg actgacagag cggttatgcc ggtgcggttg
      301 gtcgtcccct tgcctgcgat tcagggtcgc atcaccagca tgcccggttt tttaaccgac
      361 gacaaagccc tcggcatgaa agtcgtcact tattttcagc aaaacccgaa acgccatctt
      421 cccgcgattc tcgccacaat catgatgttc gatgccgata ccggtaaaat catcgcgatc
      481 atggacggca gttatgttac ggccattcgc accgcctgcg cctcggcaat ggcgacgaag
      541 gttttggcaa acccggatag caaggtggtc ggaatcttgg gagcgggcgt tcaggctcgg
      601 gcacacatcg aggctttgac gcgcgtaaga aatattggcg cgttcaagat ctatagcccg
      661 tccggcgtca gtgctgcgaa gcttcaagaa gagatgagag gacgcattga ctcggccatc
      721 gaagttgtaa gcagcgctga gcaggccatc cgcgatgccg atcttgtcat taccgtgacc
      781 accgccaaag caccgatcat caaagctgac tggctcaaac cgggcactca cattaatgct
      841 gtcggctcgc accggccgga cctcagggaa ctcgatggcg cgaccttggc ccgcgccaaa
      901 gtggtcgtcg attcgcgcgc ggcggttatg gctgagtgcg gagatatcct gcttgcaatc
      961 caggaaagaa gcatcgcgcc gagcgcggtt cacgcggaaa tcggcgaagt acttgctgga
     1021 accaagcctg ggcgaacgaa cgagagcgaa atcacgctct acaaatcggt cggcattgcc
     1081 attcaggatg tcgctgctgc ccatttgatt tacaacaaag cgctgcagca aggcgtcggt
     1141 ttagccgtcg agatttagtc gacccgagac gacctgaact tcgcgatcac gaaactccaa
     1201 ccgttgagca tatgacctgg gatctcacca gctattttcc aagtttcggc gggccggaaa
     1261 tgctgcaatt taaacaagcg ctccgcgagg acattgcagc tttacagaaa accgcagcgg
     1321 cgcttccgca attgaatgtg gattcggttt cagcctggga agatgttctc ctccgtaacg
     1381 agaaattaat gcggcgcatg tcgcacctta gctcatacat cagctgcctc gcttctgccg
     1441 atggccgaaa tgaatcgtac cagagagagg aagccgagat catccgcgat cgcgccgagc
     1501 ttggcaaagt gcgcatcgag ctcttccgtg ctttcaaagg cgtcaccgat gaggtattcg
     1561 ctgtgctgtt caaaaagccg tcgctagtcg gggcggagaa ttatttgcac cgtctgcgcg
     1621 aagaagccca tcgcgcaatg tcgagcgaca aagaagttct cgccacagaa cttggcgtcg
     1681 acggcattca gggctggggg cagctttaca acaccctatc gtccaaactt caattcgaaa
     1741 tggagtttcc cgacggctct cgcaaaattt tgccgatctc ccagcggcgt tctttactcg
     1801 atcatccgga tcggcgtatt cgggctgcgg ctttcgctag cggcaataag gcttggcaaa
     1861 gcatcgcaga caccgcggcc agcgcgctga acgcgatcgc cggcactcgc ctaacgctta
     1921 accgtcatcg cggcgtcaat cacttcctcg acatcgccct gtttcaagcc gcgatcacgc
     1981 gcaaaactct cgaggccatg ttcgatgcgc tgtttgcgaa tatagaaatt ccccggcgta
     2041 ttttgcgttt caaagcaaac ctgatgggcc gccagtcgct tgcttggtat gaccttggcg
     2101 cgccgctcga tctctcccaa gaatcagctc ggtcatggga tgaagctgtt gcgatggtca
     2161 gcgcggcatt cacgcgcgcg taccccgccc taagtagatt ctttcagcaa gaggtaatcg
     2221 gaaaaaactg gattgactgg gagccgcgcg acggcaagcg ctccggtggg ttttgcacca
     2281 gctcgatgct gagcaaagaa tcgcggattt tcatgaccta cagtcaagca ctcggtgacg
     2341 tcctcacgtt ggc
//
LOCUS       DASULZ010000130         4845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_523716, whole genome shotgun sequence.
ACCESSION   DASULZ010000130 DASULZ010000000
VERSION     DASULZ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523716"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="VFY96_02630"
     CDS             complement(<1..1096)
                     /locus_tag="VFY96_02630"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX5605379.1"
                     /translation="MKISDVQTIRLRAPIPAEGQVFSRSGVRSTRSTTLLRVETDEGI
                     SGIGSASGNADLIELIVARVIKPLIVGMDPTDIEAMWDKAYVRGGHKEFGSRGIGVVA
                     LSGVDIALWDILGKVRGLPVYQLLGGKCRERVPVYATALYPEEPAKVACRARAFAEQG
                     FYGVKIKVGFDLDQDIRIVRAVRNELGKNFTVMTDANQGYSIDVALKAADAFADCGAY
                     WLEEPLFVEDIEGHTILRERGRTPIAVGENLHTRYAFENFIVRGAVDFIQPDVARAGG
                     ISEIRKITALASTYKVPVSLHTWGDGVALAASVHLSAALENCIMMELDYTYNPLREEL
                     LREPFQVENGCVIPPERPGLGIELDQNALRR"
     gene            complement(1102..1536)
                     /locus_tag="VFY96_02635"
     CDS             complement(1102..1536)
                     /locus_tag="VFY96_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5605380.1"
                     /translation="MEATTFKLRTPHITGGRSHIPLAQTDHMTVGLNYYIPGRKNKLH
                     THPGEDHIFVVMDGQATFYNKEHQPTVLNKGEGIMLPENHYYYFASTGDVPLALFRVN
                     AKKGNKPKVVRVDAEGNKRLDEEIDFIVVDGEVVEGQFWEMK"
     gene            1686..2594
                     /locus_tag="VFY96_02640"
     CDS             1686..2594
                     /locus_tag="VFY96_02640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5605381.1"
                     /translation="MAHIGLAWVNPAPLTEPKNIVNFAKKCEAMGCHSMWTIDRIAYD
                     NLEPLTVLAAAAGATQRIRLGTSVLLGNLRHASHVAKIVSTLDFISNGRVTLGLGFGS
                     RESDYKAVEIPFEHRGSRAVEQVQLMKRLWTEDNVTFKGKFYNAENLSVGPKPIQKPH
                     PPIWTGGSAEVALKRAGSWADGFICGSSAIPEFPSTWEKIAGYARAAGRDPNRISKAG
                     LTFMAIHDDESKAVRAVEDYVMRYYGRLRADVANTSLVGSPAAVSERIGAFLAKGLDT
                     LIIGVADPDPRQLDLFGEKVIPQLSQ"
     gene            2668..3120
                     /locus_tag="VFY96_02645"
     CDS             2668..3120
                     /locus_tag="VFY96_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605382.1"
                     /translation="MKVIVIIALTAVMSLVGSSFASGPRHGGVVIRPFFPRAPVRGTF
                     GHAPFRFFPNRVVMPSRFSFTPGWYGSYYYARGYYSPPYAYAPPYPYYSYPAYGYPPV
                     PPPPAIDTSYDRGYSEGYTRGYEEAQKEINRLLDLEKTPSKEHEKSGD"
     gene            complement(3281..3460)
                     /locus_tag="VFY96_02650"
     CDS             complement(3281..3460)
                     /locus_tag="VFY96_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605383.1"
                     /translation="MPNLELTPDEALLLKEILQSHLGDLRMEIAGTDLQSFRDKLRKN
                     EEVINRIIDHLGKMD"
     gene            complement(3574..4017)
                     /locus_tag="VFY96_02655"
     CDS             complement(3574..4017)
                     /locus_tag="VFY96_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605384.1"
                     /translation="MFIETYYDETRSGFDGTGERLIPYPELVGEELAAWRQFLPIKHP
                     FGMYSWELGVPAPVLSEVRLLRAARLFDKVEIWARKDPDPMAVGIIAPASGRTRYYSI
                     ARWGDAEITLDEVKRKLRLQEVVSGAILSAAILLVMVSIFLVFSA"
     gene            complement(4563..>4845)
                     /locus_tag="VFY96_02660"
     CDS             complement(4563..>4845)
                     /locus_tag="VFY96_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013960298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605385.1"
                     /translation="NRAGDHPENALRLWKRFFDDLYKESALGPMFTTFQLHPYVSSRL
                     GRTSALKEMIEYMKGHEGVWFATGIEIANWWLEQNFSEQPAPLRMAAAS"
BASE COUNT         1086 a         1320 c         1299 g         1140 t
ORIGIN      
        1 aacgacgcaa tgcgttttga tcgagttcga tgccgagacc gggcctctcc ggagggatga
       61 cacaaccgtt ttcaacctgg aacggctcgc gcagcaattc ctcgcgcagc ggattgtagg
      121 tgtagtcgag ctccatcatg atacagtttt cgagcgcggc cgagagatgc acgctcgcgg
      181 caagcgctac accgtcaccc caggtgtgaa gggacactgg aactttgtag gtcgatgcaa
      241 gggcggtgat ttttcttatt tcggagatac cgccggcgcg cgccacgtcg ggctggatga
      301 aatcgacggc gccgcggacg ataaaatttt cgaaggcgta gcgagtatgc aagttttcgc
      361 cgacggcaat cggggttcta cctctctcgc gcaaaatcgt atggccttca atgtcttcga
      421 caaaaagcgg ctcttcgagc caataggcgc cgcagtctgc gaacgcgtcg gcagctttga
      481 gcgcgacatc gatactgtac ccctggttcg catcggtcat gacggtgaaa tttttgccca
      541 actcgttacg taccgcacgg acaatgcgga tatcttgatc gagatcgaaa ccgactttga
      601 tcttgacgcc gtaaaaaccc tgctcggcga aagcgcgcgc ccggcatgct actttggccg
      661 gttcttcggg atagagcgcg gtggcgtaaa ccggaactct ctcgcggcac ttgccgccga
      721 ggagctgata gaccgggagg ccgcgcactt ttccgagtat gtcccataac gcgatatcca
      781 cgccgctcaa agccacgacg ccgatgccgc ggctgccgaa ttctttgtgg ccgccgcgca
      841 cgtacgcttt gtcccacatc gcctcaatgt cggtgggatc catgccgacg atcagtggct
      901 tgatcactct ggcgacgatt agttcgataa ggtcggcatt accggaggcc gagccgatgc
      961 ccgaaatacc ttcatcggtc tcgactcgca ataatgtggt ggaacgcgtg ctgcgcacac
     1021 cggaacgact aaagacttgc ccttcggccg gaatcggcgc gcgcaaccga atcgtctgaa
     1081 cgtcgctaat tttcattaac gttacttcat ctcccagaat tgcccctcga cgacttcacc
     1141 gtcgaccact ataaaatcaa tttcttcgtc cagtcttttg tttccttcgg catcgacccg
     1201 caccactttc ggtttgttgc cttttttggc attgacccga aacaacgcca gcggcacgtc
     1261 gccagtgctg gcaaaataat aataatgatt ttccggcagc atgatgcctt cgcctttgtt
     1321 gagcacggtc ggttggtgtt ctttgttgta aaaagtcgct tggccgtcca tcaccacgaa
     1381 aatatgatcc tcgcccggat gggtatgtaa cttattcttt ctgccgggaa tgtagtagtt
     1441 gagcccgacg gtcatgtgat cggtttgcgc aagaggaata tgactgcgcc cgcccgtgat
     1501 atggggtgtt ctcagtttga aggtcgttgc ttccattttc attcctccta acgactcgta
     1561 tatcgcagag ccgaaatctt gaaaagagcc acaagcctgg tctatttttg cgttggtcgg
     1621 acatttgaac gggttcaact cgttgaaagg ctttgaacgc cgcaacgctt acgtaaggag
     1681 aaacaatggc tcacatcggc cttgcctggg tgaatcccgc gccgctcacc gaaccgaaga
     1741 atatcgtcaa tttcgcgaaa aaatgcgaag cgatgggctg ccactcgatg tggacgatcg
     1801 atcggatcgc ctacgataac ctcgagccgt taacggtatt ggcggcggca gcgggcgcga
     1861 cgcaaagaat tcgcctcggc acatcggtat tgctcggcaa tttgcgccat gcgagccacg
     1921 tcgcgaaaat cgtttcgacg ctggatttca tttccaacgg ccgggttact cttgggcttg
     1981 gattcggcag ccgggaaagc gattacaaag ccgttgagat tcccttcgag catcgcggta
     2041 gccgagcagt ggaacaggtg caattgatga agcgtctctg gaccgaggac aacgtcacct
     2101 tcaaaggaaa gttttacaac gcggagaacc ttagcgtcgg gccgaagccg attcaaaaac
     2161 cgcatccacc gatctggacg ggcggcagcg cagaagtggc gctgaagcgc gccggcagtt
     2221 gggccgacgg atttatttgt ggcagctcgg cgattcccga gtttccctct acgtgggaaa
     2281 aaatcgccgg ctatgccaga gccgccgggc gagatcccaa cagaataagc aaggctggcc
     2341 tgactttcat ggcaatccat gacgacgaga gcaaagcggt gagagccgtc gaagattacg
     2401 tgatgcgcta ctacggccgg ctgcgcgccg atgtggcgaa cacttctctg gtgggttcgc
     2461 ccgcagccgt cagcgagcgt atcggcgctt ttctcgccaa aggtctcgat acgctgatta
     2521 tcggcgtggc cgatccggat ccgcgacagt tggatctttt cggcgaaaag gtgattcctc
     2581 agctaagtca gtaagcggga gcgccgctca aaagcgcgtg atctatcatc gtcgaaggat
     2641 ttgctgaacc tttttttgga ggacgctatg aaggttattg taatcatcgc gctgaccgcc
     2701 gtcatgtctc ttgtgggctc gtctttcgcc tcggggccgc ggcacggcgg tgttgtcatt
     2761 cggccttttt tcccgcgcgc tcccgtcaga ggcacttttg gccacgcgcc atttagattt
     2821 ttcccaaatc gggtggtgat gccatcgcgg ttctctttca ccccaggttg gtatggttct
     2881 tattactacg cgcggggtta ttactctccg ccttacgcct atgcgccgcc gtatccgtat
     2941 tatagttacc cagcttacgg ttatcctcct gttccgcctc cgccggcgat cgatactagt
     3001 tacgatcgcg gttattcaga gggatacaca cggggctatg aagaggctca aaaagaaatc
     3061 aatcgactgc tcgacttgga gaaaacaccg agtaaggagc acgagaaaag cggtgattga
     3121 agggcggtag tttcgttaca cgtgatggac tcgcgattac tttgttcggg attaccgttc
     3181 gatcaaagat cccgtgacct gacgttattt ggagacggca gagccgaaaa gctcgcaccg
     3241 acgcggaaat tttaaaacac gtagcatgtt cagtacgtgg ctaatccatt ttccccaaat
     3301 ggtcgatgat tcggttgatg acttcctcgt tttttcttag cttgtcgcga aaactttgaa
     3361 gatcggtgcc ggcgatctcc atgcgcaaat cgcccagatg gctctgaaga atttctttca
     3421 gcaatagcgc ctcgtccggt gtaagttcca ggttcggcat atcatctgcc ttgatggcgt
     3481 atgaagcact gacgccgaga ttgaatgcgc cctgattgcg gtagcagctc tgcgcatcta
     3541 aagccggaag agcagctctg attaggcatt gctctacgcc gagaaaacca aaaaaatact
     3601 caccatgacc aacaaaatcg ctgcgctgag aatcgccccg gagaccactt cttgcagccg
     3661 cagctttctt ttcacttcat cgagggtgat ctctgcgtca ccccagcgcg caatcgaata
     3721 gtaccgtgtt ctgccgctcg cgggagcgat tattcccact gccataggat caggatcttt
     3781 gcgggcccat atttctacct tatcgaacaa tcgagctgct ctcaggagcc tcacctccga
     3841 cagcaccggc gcgggcaccc cgagctccca tgaatacatt ccaaacggat gtttgatcgg
     3901 cagaaactgg cgccatgccg ccaactcttc tcctacgagt tcaggatatg gaatcagccg
     3961 ctcacccgtc ccgtcgaagc ccgatcgcgt ctcatcgtaa taggtttcga tgaacataaa
     4021 cttctcctca ccacgatttc tgatggacct tcaagcatcg gctcgtctat tacactggcg
     4081 agcagaatgc atgccacttg ttatgcgttt tcatgacgca gacatgaacg cctgtgcgca
     4141 tggctcggcg cgatcgtcat caatagcttt gtaggagttc agattcttgc gagcagaatg
     4201 tgtcgaaaac gaaacaaggt cgcagaaatt ttttcccgag aatgcaaaat ttctttcggc
     4261 tagcggcgct gaacactatg agcaaactct cggctttgac tggagcaggg ttgaacagtt
     4321 gcaaggtgaa atcatctatc aaacacgaga aaaagcggcg attaggacac actgatacca
     4381 atgttaccgt tcagggaagc cgagatcggc tccgtacggg gttttcctct tagtgctgaa
     4441 gcgctctcga tgcaggactg actggcaatg acaccggcaa acggagatat ttggcaatga
     4501 gcggaagacg cgcatcgggc atggcgcgtc ttccgcctac gagcagatga gtggcggagt
     4561 tcctaggatg ccgcagccat gcgcagcggc gcgggctgct cagaaaaatt ctgctccagc
     4621 caccagttgg cgatctcgat cccggtcgca aaccacacgc cttcatgacc tttcatgtat
     4681 tcaatcattt ctttgagcgc gctggtcctg cccaatctgc tggaaacgta cgggtgcagt
     4741 tgaaacgtag taaacatcgg cccaagcgcc gattctttat aaagatcgtc gaagaaccgt
     4801 ttccataaac gaagtgcatt ctcgggatgg tcgccagctc ggttt
//
LOCUS       DASULZ010000131         2313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_929336, whole genome shotgun sequence.
ACCESSION   DASULZ010000131 DASULZ010000000
VERSION     DASULZ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929336"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1457
                     /locus_tag="VFY96_02665"
     CDS             <1..1457
                     /locus_tag="VFY96_02665"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX5605386.1"
                     /translation="ALEIVGSRVLAPYFGSSIFVWGSLISVVMTALSIGYYWGGWLSA
                     REPSYGKLLILLLIPGVMIFFLPLMYPSINEWIASIDFGHRLNPLIACGALFLLPGIF
                     LGTISPYVIRLAATQLHTVGSTAGTLYAVSTCGSIVGTLLTAFYLIPVLGVSNIIHIL
                     GVTLVCLSLIVVPLLRLQGVSLSRAIATVSLFLASLNLIWTPATWAKTLLQKDTFYHR
                     IRIEEDDQARYMYFDRTLQSAMNLKDPTALRLIYSRYTSLGFAFRPDAKKMLIIGLGG
                     GSIPKKVQKEFPDIEIDAVEIDPEVIKMAKDFFNVKESNNLHIHAQDGRLFLTRTPKQ
                     YDIILLDAYFTDAMPFHLTTKQFFELAQKKLTPNGIIVANLISAVTGPSGKIARAFVR
                     TQRQIFPQAYVFAARRPDHASLDTIQNVIVVATRDKQRLDIKEIVKRATAIDRGLFPD
                     PIQDIAVAYFDKPLPEDVPILTDDYAPTDNLLNP"
     gene            1516..2076
                     /locus_tag="VFY96_02670"
     CDS             1516..2076
                     /locus_tag="VFY96_02670"
                     /inference="COORDINATES: protein motif:HMM:NF025068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HEX5605387.1"
                     /translation="MRVQVLADVSFRARNPNWRDEARDLIESASDYYEREFDIRFIAQ
                     TVSAWPEKDRVRSTPELLARVKSQFGPEITNDYDLIVTFTAEGISRVVTAGRPRVDRI
                     GNCNQGLARYAVLPVTKIFQYQGANSEPEFDVLALIHELGHVFGAEHVEDTNSIMHED
                     FGYRTEFDAKNRAIIQKNRFCAFARK"
     gene            2153..>2313
                     /locus_tag="VFY96_02675"
     CDS             2153..>2313
                     /locus_tag="VFY96_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEX5605388.1"
                     /translation="MLSNLRVILVRPRGSGNIGSVARAMKNVGARELAIVGQARTRSF
                     WARAMAVHA"
BASE COUNT          490 a          653 c          588 g          582 t
ORIGIN      
        1 tggctctgga aatcgtcggc agccgcgtat tggcgcccta tttcggcagt tccattttcg
       61 tttggggtag tctgatttca gtcgtcatga ctgccttgag cattggctat tattggggcg
      121 gctggctctc agcgcgcgaa ccctcctatg gcaagcttct catccttctg ctgattcccg
      181 gggtcatgat ttttttcctg cctttgatgt atccgtcgat caacgaatgg atcgcgagca
      241 ttgattttgg tcatcggctg aacccgctga tcgcgtgcgg cgcgctgttc ctcttaccgg
      301 gaatcttcct gggcacgatc tcgccttatg tgattcgcct cgctgccacg caattgcaca
      361 ctgttggcag taccgcgggg acgctctatg ccgtttcgac ttgtggcagc atcgtcggca
      421 ctctgctgac ggcgttctat ctcattcctg tgctcggcgt cagcaatatc attcacattc
      481 tcggcgtcac tctggtatgt ctgtccctga ttgtagtacc gttgctccgg ctccaaggcg
      541 tttcattgtc acgcgcgatc gcgacggtgt cacttttctt ggcgtcactc aacctgattt
      601 ggacgcccgc gacatgggcg aaaacacttt tgcaaaaaga tacgttttac catcgcatcc
      661 gcatcgagga ggatgaccaa gcgcgctata tgtacttcga ccgcacgctg cagagcgcaa
      721 tgaatctgaa agatcccacg gcgctacgtt tgatctattc gcgctacact tccctggggt
      781 tcgccttccg gcccgatgcg aaaaaaatgt tgatcattgg cctcggcggc gggtccattc
      841 cgaaaaaagt ccagaaagaa tttcccgata tagaaatcga tgcagtcgag atcgatcctg
      901 aagttatcaa aatggcaaaa gatttcttta acgtaaagga atcaaacaat ctccacatcc
      961 atgcgcaaga cggccggctg tttttaacgc gtacgccgaa acagtacgat attattctgc
     1021 tcgacgccta ttttaccgat gccatgccgt ttcacctgac gaccaagcaa ttttttgagc
     1081 tggcgcaaaa aaaactcaca ccgaacggta tcatcgtcgc caatttgatc agcgcggtga
     1141 ccggtccttc aggaaagatc gcccgcgctt ttgttcggac ccagcgacaa atttttccac
     1201 aggcatatgt gtttgccgca cgccgccccg accatgcgag cttggacacg attcaaaacg
     1261 tcatcgtggt cgccacgcgc gacaaacagc gcctcgatat caaggagatc gtcaaacgcg
     1321 cgaccgccat cgacagaggg ctttttccgg atccaatcca agacattgcg gtcgcttact
     1381 tcgataaacc tcttcccgaa gatgtcccga ttctgaccga tgattacgcg ccgacggata
     1441 acctgcttaa tccttagcgc gctcttcatc tcctcctgtg ccgtttttca ttccgctaat
     1501 cagcaccagc gggccgtgcg cgtccaagtg ctggcggacg tttctttccg ggcacgtaat
     1561 ccaaactggc gcgacgaagc gcgcgacctg atcgagtccg catcggacta ttacgagcgc
     1621 gagtttgata tccgcttcat tgcgcagacc gtttccgcct ggcccgagaa agatcgcgtg
     1681 cgctctacac cggaattgct cgcccgagtc aagagtcagt tcggccctga gataaccaat
     1741 gactatgact tgatcgtgac ttttactgcc gagggcatca gtcgggttgt gacagccggc
     1801 cgaccgcgtg tcgatcgtat tggtaattgc aaccagggtc ttgcacgcta tgccgttttg
     1861 cccgtgacga aaatctttca gtaccagggc gcaaacagcg aacccgagtt tgatgtgctc
     1921 gctttgattc atgagcttgg ccacgttttc ggcgccgaac acgtcgaaga tacaaattcg
     1981 attatgcacg aagacttcgg ctaccggacc gaatttgacg ccaagaaccg cgcgatcatc
     2041 cagaaaaatc gtttttgcgc atttgccaga aagtaagcag taggcaatag taattaggcg
     2101 ttctgaaaga gggttgccgt ctagtcgtgc ctaacgcttg gtgcctggat ttatgttgtc
     2161 taaccttcga gtgattcttg ttcgtccgcg cggctccggc aatatcggct cggtggcgcg
     2221 cgccatgaaa aatgtcggcg ctcgtgagct cgccattgtc ggacaagcgc gcactcgcag
     2281 cttttgggca cgcgcgatgg ccgtccacgc tag
//
LOCUS       DASULZ010000132         6169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1233686, whole genome shotgun sequence.
ACCESSION   DASULZ010000132 DASULZ010000000
VERSION     DASULZ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6169
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233686"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="VFY96_02680"
     CDS             complement(<1..1286)
                     /locus_tag="VFY96_02680"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5605389.1"
                     /translation="MARPRPTVLIVDDDGATRAGLAVLVENWGYQPIEAADGKSAVKI
                     CNEELPRAIVTDLMMPGINGLEFVTALGDRARQIAIVFVTGQATIDSAVQAIKLGAYD
                     YLPKPLEPQRLREVLEKGLKQVSLAREAGALRERLESPLGSYGALIGKSSPMRRLYQQ
                     IGQIAPTDAAVLVSGESGTGKELVAQTLHDLSAQREGNFLALNCAAISPTLMESELFG
                     HEKGAFTSAVDRHVGCFEQADGGTLFLDEITEMALEIQPKFLRVLEEGQIRRIGGNKN
                     IPVSVRVIAATNRQPAAAVKEGKLRQDLFYRLNVFHLELPPLRERGEDIELLAGYFLE
                     TFAKKYNRSTLPWDTDFAPALRAHSWPGNVRELRNAMERLTVLTTGSHLTAKDFQQYC
                     LATPVEEQSEFAPLSLAEAERQAIDRALGATSGNKT"
     gene            complement(1286..2824)
                     /locus_tag="VFY96_02685"
     CDS             complement(1286..2824)
                     /locus_tag="VFY96_02685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5605390.1"
                     /translation="MSLRQQQVTVFVGTVIVIFGIFGAGIWWINQRLTTETTLQTALL
                     MARQVEIALADSLRERSVVVESRQSGGTQSSFWNFLGNLLPGRSPARSTTTTTRYAPP
                     RHAEVTGLMQAFVDRSASIEAMWVVNGEGRLLYSSVEGEKNKPAAADSVMEKLRNGEP
                     IIAAKRQGSIDYYDVWVPLQMPSGVRGPGGLRLWINPADWTGLLSGLWHQVLLLFVLG
                     GGVALVSAFLTTALYTRRFRLISDTLRQAEAGTYAHRPQYASHDEVGASLDLIDRLVM
                     KQRQNVGVPAPVQRLAIAARTLAHEVKTPLNALAIHLEVLRSKLAVDASDGAQSLRSI
                     TALDSSLRQVDRLVRDFTDYSAPVTMEKKPVDVAEVLAMSLESVRADCAAKKINLAAE
                     PLRKPWPIQGDATRLRQTFDNLLRNAIEAQPNGGDITVETKTNGDQLIVDISDHGPGV
                     PPERRLEIFEFGKTSKAGGSGIGLPLSQLIVEAHGGSLSYRDRNNDGSGATFRVSLPM
                     ELIH"
     gene            2927..3937
                     /gene="moaA"
                     /locus_tag="VFY96_02690"
     CDS             2927..3937
                     /gene="moaA"
                     /locus_tag="VFY96_02690"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX5605391.1"
                     /translation="MLTDSLKRPLKDLRISVTDRCNFRCTYCMPLDHYEWIDKKEILT
                     FEEISRLARLAVELGVEKIRLTGGEPLVRHDLPTLVENLASIAGLKDLCLTTNGALLA
                     EQVDALKSAGLRRVNISVDTLDAEKFRSMTKRGDLDKVLEGIFAAKEAGLAPIKLNAV
                     VERGVNEDDIIPLVEFARQHGLSMRFIEYMDVGNSNRWTSAKLVAKKDIIAKINARYP
                     LREVGRFEGSAPAVDYEFVDGAGDIGVIASVTEPFCGSCTRLRLTADGKLVTCLFSEH
                     GQDVKALLRGGATDDELRTFLSSLWQKRSDRYSMERLEALNSANYDPASHRKIEMISL
                     GG"
     gene            complement(3951..4229)
                     /locus_tag="VFY96_02695"
     CDS             complement(3951..4229)
                     /locus_tag="VFY96_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605392.1"
                     /translation="MKISRCRYRITLFAGFSLAVSGCTSQTVKMIQPQTGATANCSGS
                     SYGFGQLFTESFVDSCARAYQDRGFVPLERLTPEARADLDKRGLLPKD"
     gene            4400..5143
                     /locus_tag="VFY96_02700"
     CDS             4400..5143
                     /locus_tag="VFY96_02700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5605393.1"
                     /translation="MLLKDKIALVTGSTHNIGLAIARAFAREGAKVIIHSRHEADAKQ
                     IAAGLSGDYFTADLADPAQIAALFEHVQRTHGRLDLLVNSVAHSTKGGVLDTTLEEWR
                     DVLAINLTGYFLCIQHAGKMMMAQGQGSIINISAGSGERSSPGGAVYSVSKGAINALT
                     KQAAADLAPKIRVNGIISGLVGTPLGQKEMGNRKPEYDFIPLKRIGRPEDVAEAAVFL
                     ASDKASYITNTILPVDGGRLNSMNSGSAR"
     gene            complement(5187..5954)
                     /locus_tag="VFY96_02705"
     CDS             complement(5187..5954)
                     /locus_tag="VFY96_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX5605394.1"
                     /translation="MECSHAQSLLEIYLDQELDPVRHLEIEEHLHGCAGCAQSYGDRQ
                     VIRQSLKAESLYFKAPPKLEKRIQRSLRQAAKAETPARWLSGSWVKMAAPLAVAALVV
                     LMLVPHFSGPSPDDLLTQQVIASHIRSLMVDHLADIPSTDEHTVKPWFNGKLDFSPPV
                     ADPVKGGFPLIGGRLDYLNNRPVAALIYRRDKHLINVFVWPASTGQQLAQSHKTQQGY
                     NLIRWTSSAMNFWAVSDLEANQLTKFSELLKSSIPSS"
     gene            complement(5956..>6169)
                     /locus_tag="VFY96_02710"
     CDS             complement(5956..>6169)
                     /locus_tag="VFY96_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007984247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5605395.1"
                     /translation="KSGDVEMIRQSLQELPDEFREVIVLRDLEEMPYKQIAAVIKAPL
                     GTVMSRLARARAKLKRILCSRLKEKP"
BASE COUNT         1332 a         1796 c         1707 g         1334 t
ORIGIN      
        1 tgggttttgt tcccgctggt ggcgccaagg gctctgtcga tggcctggcg ttcggcttcg
       61 gcgagtgaca gcggcgcaaa ctcggactgc tcctcgacag gcgtcgccaa acaatactgc
      121 tgaaaatctt tcgcggttaa atggctgccg gtcgttaata ccgtcagtcg ctccatggca
      181 ttgcggagct cacgtacatt accaggccag ctgtgggctc ggagcgctgg cgcaaaatcg
      241 gtgtcccaag gcagggttga acggttatat ttttttgcga atgtttccag gaaatatccg
      301 gccagcagct cgatgtcttc accgcgctcg cgcagcggcg gcagctcgag gtggaacaca
      361 ttgagccggt aaaacagatc ctggcgcagc ttgccttctt tgaccgccgc ggccggttga
      421 cggttggtcg ccgcgatcac gcgcactgac acaggaatat tcttgttccc gccgatgcgc
      481 cggatttgac cttcctcgag cacgcgcaga aatttcggct ggatttccag cgccatctcg
      541 gtgatctcat cgagaaaaag cgtgccgccg tcggcttgct cgaagcaacc cacgtggcga
      601 tcgacagcgc tcgtgaacgc gcctttttcg tgaccgaaca gctcgctttc catcaacgtg
      661 ggcgagatcg ccgcacagtt gagcgcaaga aaatttccct cccgctgcgc cgacaaatca
      721 tgcagcgttt gcgccactag ttccttacca gtgccgctct cgccgctcac cagcaccgcg
      781 gcgtccgtgg gcgcgatctg gccgatctgt tgatacaggc gtcgcatcgg ggaagatttt
      841 ccgatcagag ctccgtacga gccgagcggc gattccaacc gctcacgcag cgcgcccgcc
      901 tcccgtgcca gcgacacctg ctttaaacct ttttccagga cctcgcgcag ccgctggggc
      961 tcgagcggct tgggcaggta atcgtaagcg ccgagcttga tcgcctgaac ggcagagtcg
     1021 atggtcgctt gcccggtgac aaaaacgatc gcgatctggc gcgcgcgatc gcccagcgca
     1081 gtcacgaact ccaatccgtt aatccccggc atcatcagat ccgtcacgat cgcgcgtggt
     1141 agctcctcgt tacagatttt cacagccgac ttaccatcgg ccgcttcgat cggctgatag
     1201 ccccaattct cgacgagaac agccagaccg gcgcgcgtcg cgccgtcatc atccacgatc
     1261 aagacagtcg gtcgcgggcg ggccattagt gaatcaactc cattggcagg gaaacgcgaa
     1321 aagtcgcgcc gctcccgtcg ttattgcggt ctcggtaact gagagaacca ccgtgggctt
     1381 cgacgatgag ttggctcagc ggtaagccga tcccgctgcc gcccgctttg ctggttttgc
     1441 cgaactcgaa aatttccaag cgccgctccg gcggcacgcc gggaccgtga tcgctgatat
     1501 ccacgattaa ttgatcgcca ttcgttttgg tctcgactgt aatgtcgccc ccgttcggtt
     1561 gggcttctat cgcattgcgc agaagattat cgaaagtttg ccggaggcgc gtcgcatcgc
     1621 cttgtatcgg ccaaggcttg cgcagcggct cagccgccaa attgatcttt ttggcggcac
     1681 aatcggctct gacgctttcc agactcatgg ctaaaacctc ggccacatcg acgggttttt
     1741 tttccatggt gacgggcgcc gagtaatcgg taaaatcgcg cactaaccga tcgacttgac
     1801 gaaggctcga atcgagtgcc gtgatggaac gcagcgattg cgcgccgtcg ctcgcgtcga
     1861 cggcaagttt gctgcgcaat acttctaaat gaatcgccaa agcgttcaaa ggcgtcttta
     1921 cttcgtgggc aagcgtgcgg gcggcgatcg ccaggcgctg caccggcgcg ggcacgccga
     1981 cattttgccg ttgtttcatc actaaacgat cgatcagatc caagctcgcg ccgacttcgt
     2041 cgtgactggc atactgaggc cggtgcgcat acgttcccgc ttcagcctgg cgcaaggtat
     2101 cgctgatcaa acgaaacctg cgcgtgtaca gcgccgtcgt cagaaaagca ctaactagcg
     2161 caacaccgcc gccgagaaca aacagtaaga gtacctggtg ccaaagtccg gacaacaatc
     2221 ccgtccagtc ggccgggtta atccacagtc tcaagccgcc cggaccgcgc acgcccgacg
     2281 gcatttgcag cggcacccaa acatcgtagt agtcgatgct tccctggcgt ttggcggcga
     2341 tgatcggctc accattacgc agtttttcca tcacgctatc tgcggcagcg ggcttatttt
     2401 tctccccctc caccgagctg taaagcaaac gcccctcacc gttgactacc cacatcgctt
     2461 cgatgctcgc gctgcgatcg acaaatgcct gcatcaaccc ggtcacttcg gcgtggcgcg
     2521 gcggcgcata gcgggtcgtc gtggttgtcg atctggcagg gcttcgacca gggaggagat
     2581 tgccgagaaa gttccaaaaa gaggattgcg tccctcctga ctggcgcgat tcgacgacga
     2641 ccgagcgttc tcgcaggcta tcggcgaggg cgatttcgac ctgccgcgcc atcagcagag
     2701 cggtttgcag cgttgtttca gtcgtgagcc gctggttgat ccaccagatg ccggcgccga
     2761 aaataccgaa tattacaatc actgtgccga caaagacggt cacctgctgt tggcgaagac
     2821 tcatggcggg cgctagaata ccagatcggc caactagccg gaagttcgct tcttgcgcag
     2881 aatcgccggc ttgcgcgctc agacccttta ttgcatagtg aattagatgc taaccgacag
     2941 ccttaaaaga ccgcttaaag acttacgcat ctcggtgacc gaccggtgta attttcgctg
     3001 cacttattgt atgcctctcg atcactatga atggatcgat aaaaaagaaa ttcttacatt
     3061 cgaggaaatt tcgcgcttag ccaggctcgc cgttgagctc ggtgtcgaga agatccgtct
     3121 caccggcggt gaaccgttgg tgcgtcacga tttgccgacc ctggtcgaaa acctcgcttc
     3181 catcgccggc ttgaaagacc tatgcctgac caccaatggc gctctcctgg ccgagcaggt
     3241 tgacgccctc aagagcgccg gactgcggcg agtgaacatc agcgtcgata cgttggatgc
     3301 cgaaaaattt agaagcatga ctaaacgcgg cgatctcgac aaagttctcg aaggaatctt
     3361 tgctgctaaa gaagccggcc tcgcgccgat caagctaaac gccgtagtcg agcgcggcgt
     3421 caatgaggac gatattatcc cgctcgtgga atttgcccgc cagcacggct tgagtatgcg
     3481 cttcatcgaa tacatggacg tcggcaactc caaccgttgg acatcggcaa agctggtggc
     3541 gaaaaaggat atcatcgcaa agatcaacgc gcgctatccg ttaagagagg tgggacgctt
     3601 cgaaggaagc gctcccgccg tggattacga gttcgtcgat ggcgccggcg atattggagt
     3661 tatcgcctcg gtaaccgaac ctttctgcgg cagttgcacc cggcttcgtc tcaccgcaga
     3721 cggcaaactg gtcacttgcc tgttttctga acacggacag gatgtcaaag ctttgctgcg
     3781 cggcggtgcg accgacgatg agctcagaac ctttttgagc tcactttggc agaagcggag
     3841 cgatcgctat tcgatggagc gcctcgaagc gctaaactcc gccaactacg atcccgccag
     3901 tcatcgtaag atcgaaatga tttcactcgg tgggtgagac cgcgctgctg tcaatccttg
     3961 ggcagcaatc cgcgtttgtc taagtcggcg cgcgcttccg gagtcaacct ctccagcggc
     4021 acaaaaccgc gatcctgata cgctcgcgcg caactatcga caaaactttc ggtaaaaagc
     4081 tgtccaaaac cataactcga gccgctgcag ttggcggtcg cgccggtctg tggctggatc
     4141 attttcacgg tttggctggt gcaaccagaa accgccagcg aaaaaccggc gaacagagtg
     4201 atgcgatagc ggcacctact gattttcatt tatctcacct catatctacc taggtctgca
     4261 tggtattagc acgtatgctc atcatccagt acatcgcact tttaaaaaac tgtcattccg
     4321 acccaagtga ggaacctttc ttcgttgctc agcccaatcg ctcgaagtat ttaaaagcaa
     4381 accgaactgg aggacactca tgttactcaa agacaaaatc gctctggtca ccggctccac
     4441 gcacaatatc ggcctggcga tcgcgcgcgc gttcgcgcgt gaaggcgcca aagtcatcat
     4501 tcattcgcga catgaggccg acgcgaaaca gatcgctgct ggattatcgg gtgactattt
     4561 cacagccgac ctggccgacc ccgcgcagat cgccgctctc ttcgagcacg ttcagagaac
     4621 ccacggccgg ctggatctcc tggtgaacag tgtcgctcat tcgaccaagg gcggcgtact
     4681 cgatacgact ctcgaggaat ggcgcgatgt actggcgatc aacttgaccg gctattttct
     4741 gtgtattcag cacgcgggca aaatgatgat ggcgcaaggg cagggcagca tcattaatat
     4801 ctccgccggt tccggagaac gatcgagtcc gggcggagcc gtatattccg tttcaaaggg
     4861 ggcgatcaat gccttgacca agcaggctgc cgctgatctc gcgccgaaaa ttcgtgtgaa
     4921 tggaataatc tctggattgg tcggtacgcc actgggacaa aaagagatgg gcaatcggaa
     4981 gcccgaatat gattttattc cgctgaagcg catcggccgc cccgaggacg ttgctgaagc
     5041 ggcggtattt cttgcttccg ataaagcgag ttatatcacg aatacgattt tgcccgtgga
     5101 cggcggccgc cttaattcga tgaatagcgg cagcgcgaga tgagcgcctg cggcgctctc
     5161 gccgaaaaag caagcggcgc ttcacgtcag ctggagggta tcgatgattt tagcagctcg
     5221 ctaaatttcg tgagctggtt ggcttcgaga tccgacaccg cccagaaatt catagcggaa
     5281 ctggtccagc gaatcaggtt atagccctgt tgcgttttat ggctctgcgc gagctgttga
     5341 cccgtgctgg cgggccaaac aaagacgttg atgagatgtt tgtcgcgccg atagatcagc
     5401 gccgcaacgg gtctgttgtt caagtaatcg agccgcccgc cgatcaaagg aaagcctccc
     5461 tttaccggat cagccaccgg aggagaaaaa tccagcttgc cattaaacca cggtttcacc
     5521 gtatgttcgt ctgtcgaagg aatgtccgcc aagtgatcga ccatcaatga acggatgtga
     5581 ctggcaatga cttgctgggt caaaagatca tcgggcgacg gtccggaaaa atgtggaacg
     5641 agcattaaaa cgaccagcgc agcgactgcc agcggtgctg ccatttttac ccacgatccg
     5701 gataaccatc gtgccggcgt ctcggctttg gcagcttggc gcaacgagcg ctggatgcgt
     5761 ttttccagtt taggaggagc tttgaaataa agcgattctg ctttgaggct ctggcgaatt
     5821 acctggcgat ccccgtagct ctgcgcacaa cccgcgcagc cgtgcagatg ctcttcgatt
     5881 tccaggtggc gcaccggatc gagctcctga tccaggtaaa tctccagcaa gctctgggcg
     5941 tgactgcact ccatgctagg gcttctcctt gagtcgggag cagagaattc tttttaattt
     6001 ggcgcgcgcc cgcgccaggc gcgacatcac cgtcccgaga ggcgccttga tcactgcagc
     6061 aatctgcttg tagggcattt cttcgagatc gcgcaggacg atgacttcgc gaaactcgtc
     6121 cggcaattct tgcaatgact gcctgatcat ttccacgtcg ccgctcttg
//
LOCUS       DASULZ010000133         2347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_218645, whole genome shotgun sequence.
ACCESSION   DASULZ010000133 DASULZ010000000
VERSION     DASULZ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218645"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1148
                     /locus_tag="VFY96_02715"
     CDS             <1..1148
                     /locus_tag="VFY96_02715"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5605396.1"
                     /translation="DLNYCKSILYLQEDSMNSPISPRIIDADAHVVECARTWDFMDAS
                     ERQYRPIPLETREEAGIKLQFWLIDGKVRGFRFPAFSTEALANLAGRVGRKFADAQEA
                     RELGNVELRLEHMDETGVDVQVLHNTVFIESATDRAGVEVALYKSWNQWLGDIWKQGQ
                     GRLLWSCMAPTLSIPDALDQIRWSKQHGACAVMMRPVEGQRLLADPYFYPIYDEAQKL
                     DLPIAIHIANGNSWLNDLYSHPVRIASTFHRFRIPTVAAFNDIIMSEIHEVFPRLRFG
                     FIEASAQWLPWVMHEARNRFKTLGRSWPENPFRTLGMYVTCENGDDIPYIVREAGEDC
                     LVIGTDYGHTDTSSDVDAIKIFKDRPDVSPEVKRKILSDNARALYGL"
     gene            1307..2323
                     /locus_tag="VFY96_02720"
     CDS             1307..2323
                     /locus_tag="VFY96_02720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5605397.1"
                     /translation="MRFYINILTTYFPNLDPPYDTYYQQILQQVELAEELGWECFMFN
                     EHHFLGYGGLVANPAVLLAAAAARTSKIRLGPCIAILPLRHPLHSAEDYAMVDAISGG
                     RLEFGIGSGNTDIDYKVFGVSRENDRQRLREAIDVILKTWANERISHTGQFWKFDELA
                     LYPRPIQQPYPPIWVAGTSAEGLGWAGGQGYHIMTVGHPHPPEKVRPGVEAWKQGLLA
                     SGYDLNTRHCQFHVRTHVNESSANARSIAMAAIKRYDEISRIGRRALTASPEDYDWNM
                     MLATGRNNYGNPVECIKNIENARKNYYFDTLTTTFNFGGIPHAEILKSMRLFAKEVMP
                     AFHP"
BASE COUNT          557 a          658 c          612 g          520 t
ORIGIN      
        1 gtgatctcaa ctactgtaaa tccattctct acctgcagga ggattccatg aactcaccga
       61 tctcccccag gattatcgac gctgatgccc acgtggtcga atgcgcacgc acttgggact
      121 ttatggacgc atcggagcgg caatatcgac ccataccgct cgaaacccgc gaagaagccg
      181 gcatcaagct tcagttctgg ctgatcgatg gcaaggtcag aggatttcgc tttcccgcgt
      241 tttcgaccga agcgttggcc aatctggccg ggcgcgtggg gcgcaaattt gccgatgcgc
      301 aggaagcgcg tgagctcggc aacgtcgagc taaggttaga acatatggac gaaaccggcg
      361 tggacgttca ggtgctgcac aatactgtat tcatcgaatc ggccaccgat cgtgccggcg
      421 tcgaagtcgc tttatacaaa agctggaatc aatggctggg cgacatctgg aaacagggac
      481 aaggacgctt gctttggtca tgcatggcgc caaccttgag catacccgat gcactagatc
      541 aaattcgctg gtcgaaacag catggtgcgt gcgctgtcat gatgcgacct gttgaagggc
      601 agcggctcct ggcagatcct tacttctatc ccatctacga tgaagcgcag aaactcgacc
      661 tgccgatcgc aattcatatc gccaacggta attcatggct gaatgacctc tatagccatc
      721 cggttcgcat cgcctcgacg tttcatcgct tccggattcc tacggtcgcc gctttcaacg
      781 acattattat gagcgaaatc catgaggtct ttccgcggct tcgtttcggt ttcattgaag
      841 cgagcgcgca gtggctgccc tgggtcatgc acgaagcgag gaatcgattc aaaaccctcg
      901 gccggagttg gcccgaaaat ccatttcgca ccttgggcat gtacgtgacc tgtgaaaatg
      961 gcgatgatat tccgtatatc gtgcgcgagg ccggcgaaga ctgtctggtg atcggcaccg
     1021 actacggcca tacggatacg tccagcgatg tcgacgcgat aaaaattttt aaagacaggc
     1081 ctgatgtgtc gcctgaagta aagcggaaga tcttaagcga caatgcgcgc gccttgtatg
     1141 gcctatgacg gcctcaagct tcaaggcgcg ccccgcccgt actgagattc caaacatctc
     1201 gcctcattca ccattcttga ttgctttcag ccgagccctc gagctctcat cggcacccgt
     1261 ttatctggat cgttgaatcg aagcacccga gaacaaccca cgcgatatga gattttacat
     1321 caacattttg acaacgtact tccctaatct cgacccgccg tatgacacct actaccagca
     1381 aattctccaa caggtggaac tggcggaaga gctaggctgg gaatgcttca tgttcaacga
     1441 acatcacttt ctcggttacg gcggcctcgt cgccaatccg gccgtcctgc tcgccgccgc
     1501 tgccgcgcgc acatcgaaaa ttcgtttggg accctgcatt gcaattttgc cgctgcgcca
     1561 cccgctgcac agtgcggaag actacgcgat ggtcgatgct atctctggcg gacggcttga
     1621 gtttggcatc ggatcgggaa acaccgacat cgattacaaa gttttcggtg tcagccgaga
     1681 aaacgaccgt cagcggctcc gcgaggcgat cgacgtgatc ctgaaaacct gggccaatga
     1741 acgaatcagc cataccggac agttttggaa attcgacgaa cttgcgctct atcctcggcc
     1801 aatccaacaa ccctatccgc cgatctgggt cgcgggcacg tcggctgaag gtcttggttg
     1861 ggcgggaggc caaggatacc acatcatgac ggttggccat ccacacccac cggagaaggt
     1921 ccgtcccggt gtcgaagcat ggaagcaggg actgttagca tcgggctacg atctcaacac
     1981 taggcactgt caatttcatg tgcgtacaca cgtgaacgaa agttcggcaa acgcgcgctc
     2041 catcgcgatg gcggcaataa aacgttacga cgaaatttcc cgaattggcc ggcgcgctct
     2101 taccgcctca cccgaggatt acgattggaa tatgatgctc gccaccggcc ggaacaatta
     2161 tggaaatccg gttgaatgca tcaaaaatat tgaaaatgcc cggaagaatt attatttcga
     2221 tacgctgacg acgaccttca atttcggtgg cattccgcac gctgagattt taaagtccat
     2281 gcgtcttttc gccaaagaag tgatgccggc ttttcacccc tgaggttcgg aacgcgcgca
     2341 caataaa
//
LOCUS       DASULZ010000134         2780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1133153, whole genome shotgun sequence.
ACCESSION   DASULZ010000134 DASULZ010000000
VERSION     DASULZ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133153"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1568
                     /locus_tag="VFY96_02725"
     CDS             132..1568
                     /locus_tag="VFY96_02725"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX5605398.1"
                     /translation="MADIFISYANEDRETAAQFAQLLESVGWHVWWDRRIPAGRTWRS
                     ILEDALREMRCLVVLWSKNSVQSPWVTEEAEEARRLGKTIVPVLIEGVEPPIGFRAIQ
                     AANLANWNGSTDDPAAQQFIADLKSILGDVVETSLPPGESSPAKTRVTTLLQRTTSAR
                     IFKFGAVIAAFVVFLVGWQVWREFSQHTQISAPVEKAAVRSAPQLASLAIRGERKNLN
                     PAEKTKLELRAIDSEGKQAQIKDAISWSSSAPQVAGVSPEGEVMALTPGTAEIKANVG
                     GVVSPPWAINVKRVEAPSKAAAQPKIVALRVTVGTRELHLNERIPIRVRGRYSDESEQ
                     ALSRDIEWQISNRAVASISDAGELEGLRPGKTEVVARYGDLRSPPVIVLVKEPEKPAQ
                     QPAKSMQTPEPGSVKVQTSTAEVKTKVTTYVGRAGSLREQGNYAGAMAELEKARILDP
                     FDEGVRKEIEQTKRACNAERVLGNPVSC"
     gene            complement(1594..2436)
                     /locus_tag="VFY96_02730"
     CDS             complement(1594..2436)
                     /locus_tag="VFY96_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605399.1"
                     /translation="MRLAHFSIVLLLIVSCAGLATQSEFLAGRQALLRGEPDNARSYF
                     DRVAQSDPRFVSNEVSPPRSIWTYIGRADYNAGRYAQAESAFEKAVGQLKEDYIAKLY
                     LGLTRLRPPEPTVSSKAFSLQEITYALREGIDPKRVATLARERGVAFDLNKETENQLQ
                     TIGANNLLLDELKKIRAENTKGSRSTDARRSQAGKELTDALTGLREWLAYTISYTSQG
                     KFWDPSERIRNQLQLCLKMLDARPPDWDALVANAEWVGSMFEEESDRARRDEAAERNR
                     QLRR"
     gene            2592..>2780
                     /locus_tag="VFY96_02735"
     CDS             2592..>2780
                     /locus_tag="VFY96_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605400.1"
                     /translation="MRFSPTPWLVASLAFVVLGFSRGIHSSFGVFNVALLDTFGWSRG
                     ATAGIFSIVLTVDAILSPL"
BASE COUNT          610 a          762 c          792 g          616 t
ORIGIN      
        1 caccaagagg tttcattttg ttagtttgca ttcgagattt tccaaaattc cgaacatttg
       61 ttagaatccg gcgcagctta tctgtccggc atctccgtca gcattcgtcg acacggtgaa
      121 agtccgggtc catggccgat attttcatca gctacgcaaa tgaggatcgc gaaacggcag
      181 cccagttcgc ccagctgctc gaatcggtcg gttggcacgt atggtgggat cgccggattc
      241 cggcaggacg cacctggcga tcgatcctcg aagatgcgct acgtgagatg cgctgcttgg
      301 tcgtgctctg gtcgaaaaat tcggtgcaaa gcccgtgggt tacggaagaa gctgaagaag
      361 cacgtcggct tggcaagacc atcgtcccgg tattgatcga gggggtcgag ccgcccatcg
      421 gctttcgcgc gattcaagcg gctaatctcg cgaactggaa cggctcaacc gatgatcccg
      481 ccgcgcagca attcatcgcc gatctaaaat ccattttggg tgacgtggtc gaaacctctt
      541 tgccgccggg cgaatcttcg ccagccaaaa cccgcgtaac taccttgctg cagcgaacca
      601 ctagcgcgcg aatctttaag ttcggcgctg tcatcgccgc ctttgtcgtg tttttggttg
      661 gatggcaagt ctggagagag ttctcacaac acacacaaat atcagcaccc gtagaaaagg
      721 ccgctgttag atccgcgccg caattggcga gcctcgcgat tcgcggcgaa cgaaagaatc
      781 tcaatccagc agaaaaaacg aaactggagt tacgcgcgat cgattcggaa ggcaagcagg
      841 cgcagatcaa agacgcgatc agttggtcga gcagcgctcc gcaagttgca ggcgtcagtc
      901 cggagggcga agtcatggct ctcacgcccg gaaccgcgga gatcaaagca aacgttggcg
      961 gtgtggtgag tccgccgtgg gccatcaatg tcaagcgagt cgaagcacca tcgaaagccg
     1021 cagcgcagcc aaaaatagtt gctttgcggg tgaccgtcgg aacccgagaa ttgcacctaa
     1081 acgagcggat tcccatccgt gtgcgcggcc gctattccga tgaaagcgag caagctcttt
     1141 cgcgagatat cgaatggcaa atcagcaacc gcgccgttgc atcgatcagt gatgccggtg
     1201 agctagaggg gttgcggccc ggcaagaccg aggtggttgc gcgctatggc gacttgcgta
     1261 gcccgcccgt gatcgtgctc gtcaaagagc cggaaaaacc ggcgcagcag cccgcaaagt
     1321 cgatgcaaac tcccgaaccg gggtcagtga aagtgcagac ttcaactgcg gaggtgaaaa
     1381 ccaaagtgac gacctacgtc gggcgtgccg gttcgttacg cgagcaggga aattacgccg
     1441 gggcaatggc ggaactggag aaggcgagaa tcctggaccc cttcgatgaa ggcgtgcgaa
     1501 aagaaattga acagacgaaa cgagcctgca acgccgaacg ggtcttgggc aatccggtaa
     1561 gttgctaatt cgatgcgact gagtcccgga actctagcgc cgtaattgcc gattacgttc
     1621 cgccgcctca tcgcgccgcg ctcgatcgct ctcctcctcg aacatcgaac cgacccattc
     1681 cgcgttcgcg acgagcgcgt cccaatccgg aggtcgcgcg tcgagcattt taagacaaag
     1741 ttgcagttga ttccgtattc gttccgaggg atcccagaat tttccttgcg aggtatagct
     1801 aatcgtatag gcgagccact cgcgcaatcc ggtcagcgca tcggttaatt ccttgccggc
     1861 ttgcgatctt cgtgcatccg tcgatcggct tcctttggtg ttctcggcac ggattttttt
     1921 cagttcgtcg agaagcaaat tattggcgcc aatcgtctgg agctgatttt cggtctcttt
     1981 gttcaaatca aaggcgacac cgcgttcgcg ggccaaagtg gcaactcgct tgggatcaat
     2041 tccttcacgt aacgcatagg ttatttcttg cagtgaaaac gccttggaag aaacagtcgg
     2101 ctcgggcggt cgaagccgtg tcaggcccag atagagcttg gcaatgtagt cttccttgag
     2161 ctggccgacg gctttttcaa acgccgattc tgcctgcgca tatctgccgg cgttgtagtc
     2221 cgcacgtccg atataggtcc atatgctccg cggcggcgaa acctcattgc tcacgaagcg
     2281 cgggtcgctc tgagctaccc ggtcgaaata ggaccgcgca ttgtcgggtt caccgcgcag
     2341 taaagcttgg cgtccggcga ggaactcgct ctgcgttgcc aagccggcgc atgacacgat
     2401 caacaaaaga acgattgaaa agtgagctaa ccgcatacaa atcgccagca gaataagcgc
     2461 agcgtggaga aaagacaagt ttttcaccct cgctgaggtg aaagatttcg cggcgcggcg
     2521 gctttgcctc tggttaatcc ttggcggttg tggctcagac aacgctctgc taagcagaag
     2581 atatttttct catgcgtttt tctcccacac cgtggctggt ggcgtcgctt gcctttgtcg
     2641 ttctaggttt ttcccgcggt attcattcat cctttggcgt ctttaatgtc gcgttgctgg
     2701 atacctttgg ttggagccgc ggcgcgaccg cgggtatttt ctcgatcgtt ttgaccgtcg
     2761 acgctattct gtcgccgctc
//
LOCUS       DASULZ010000135         3802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_219294, whole genome shotgun sequence.
ACCESSION   DASULZ010000135 DASULZ010000000
VERSION     DASULZ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219294"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VFY96_02740"
     CDS             complement(<1..190)
                     /locus_tag="VFY96_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605401.1"
                     /translation="MKKLGSVILSTALLALPVRGLAAYQAGDVKDGGSISGTVKFKGS
                     APAPKKLDVRKDKEVCDKT"
     gene            complement(252..2945)
                     /locus_tag="VFY96_02745"
     CDS             complement(252..2945)
                     /locus_tag="VFY96_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5605402.1"
                     /translation="MAERGVDLKEEKKSYGSVFVIGVALLVALSLWAYWDDNITRRPW
                     KGVQARFYRLDYNKAKAAYDEEDKKLQDDPKYQELSKKLADIQTSLARGDLAKKLQAL
                     EEQESKATVAFKDKDQEVKFIKSELEEAWYEHDHAVQEKRNPAPYLEQIKELDAEKAK
                     LDPEVEKAQARRDQIREEIKKIKSSGKDLEDELSKMSADRDKWVRVMENDTFKLGPFA
                     FYKIPKIQQVSLNEFDRNRFDQPVARVDRCQTCHLAINRAGFEKEANPFKTHPRREVL
                     LADNAHSPEKFGCTTCHEGQGVAVNSAQAAHGEVHLWEFPLLRGASAQSSCTSCHLDV
                     QKFADDAPLLVEGQRLFEQVGCTGCHLVKGYENIPKIAPSLLKISAKVDPNWMVRWIE
                     NPHKFRPRTRMPNFDLKESDAIAIASYIWSQSKEEGDKWTQEHPLPTGIRPGDANSVG
                     QGKKLVESIGCKGCHGFADGEYTTPLGKSKDLVPNLKDVAAKVVGPQWVYHWLKNPRG
                     YSPETRMPSLRLSDDEAASITSYLMTLGTKGDEIPNIQEKLADAQNIKRGEGLVRKWG
                     CFGCHDIKGMEKETRIGAELTTFGSKANEELFFGNRTDIKETWDDWTYNKLKTPRIYA
                     TERIEQLMPQFDLADEDIKALRVLLGGFRERKVGLAYLADQGLRVQQVVEGRRLMQQY
                     NCIGCHEIEKRGGYIRKYYQENPTLAPPILNGEGEKVQSNWFFSFLKAPVPIRPWLDL
                     RMPTFGLSDEHTNQLISYFNGLSKVDIPFAYVDDKKIPPDHIEAAKQLVSKDYFNCFS
                     CHQQGDKKPEGPVEGWAPDLTMARQRLNPNWILKWLRDPQKVQPGTKMPSFYPGGPDD
                     ILGGKEDLQIEALRDYLMSIGRGGPAPPAPAVAAKAKPGRS"
     gene            complement(2956..3114)
                     /locus_tag="VFY96_02750"
     CDS             complement(2956..3114)
                     /locus_tag="VFY96_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605403.1"
                     /translation="MAKILQALGLADMGYALFAGIVRNASMAEEIVVSLIGLGVFSLG
                     RVLERKVV"
     gene            complement(3124..>3802)
                     /locus_tag="VFY96_02755"
     CDS             complement(3124..>3802)
                     /locus_tag="VFY96_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEX5605404.1"
                     /translation="LFVLVALTLWALTVDAPLEEPANPTRTPNPSKAPWYFLGLQEML
                     VYFDPWNAGVVLPSLIIVGLMVIPYIDINPRGNGYYCFKDRKWEILTFFLGFHILWIT
                     MIMIGTFFRGPGWNLFWPWQKWDPHKVVALTNVDLPYLVGFRDYWWSAVFSLVMIGMY
                     FITTIALFYFFVLKVKGKAFLERWGMVRFSITAVLIALMLSLPAKMALRLLFNVKYVL
                     VTPWINV"
BASE COUNT          810 a         1082 c          947 g          963 t
ORIGIN      
        1 gtgtcttgtc gcagacctcc ttatcttttc tcacatcgag tttcttcggt gccggcgcgc
       61 tgcccttgaa tttcactgtc cccgatattg agccgccatc ctttacatcg cccgcctgat
      121 aggctgccag gccgcgcaca ggtagagcta aaagcgccgt cgacaagatc actgaaccca
      181 gcttcttcat agttcctcct tcagtataaa gactgtacca tttgtattga cgaatgaaac
      241 ccggctacta ttcacgacct tccgggtttc gctttggccg cgactgcagg cgctggtggc
      301 gccggtccac cacgaccgat actcatgaga taatcccgca acgcctcgat ttgcagatct
      361 tctttgccgc cgagaatgtc gtccggcccg cccggataaa aggaaggcat cttcgttccc
      421 ggctggactt tctgcggatc ccttaaccac ttgagaatcc agttggggtt cagccgttgc
      481 cgtgccatgg tcagatccgg cgcccaacct tccactggcc cctcgggttt tttatcaccc
      541 tgttgatggc agctgaagca gttgaagtag tctttcgaca cgagctgctt tgccgcctcg
      601 atatggtcag gcggaatttt tttatcgtcc acgtacgcga aaggaatatc gaccttggat
      661 agcccgttga agtaactgat caactggttt gtatgctcgt cggacaagcc aaatgtcggc
      721 atgcgcaggt cgagccacgg acgaatcggc accggtgctt tcagaaaact gaagaaccag
      781 ttcgattgca ctttctcgcc ttcaccgttg agaatcggcg gtgccagggt tggattctcc
      841 tgatagtact tgcgaatgta gccgcctctc ttttcgattt cgtggcaacc gatacagttg
      901 tactgctgca tcagacggcg cccctccacg acttgctgta cgcgcagccc ctgatcggcg
      961 agataggcta agccgacttt ccgctcgcgg aagcctccca gcaataccct cagcgccttg
     1021 atgtcttcat ccgccagatc aaattgcggc attaactgct caatccgctc ggtggcataa
     1081 atccgcgggg ttttgagctt gttgtatgtc cagtcgtccc aggtttcttt gatatcggtg
     1141 cggtttccga agaaaagttc ctcgttggcc ttggagccaa aggtcgtgag ctcggcaccg
     1201 atgcgggttt ctttttccat ccctttgata tcgtgacaac caaagcagcc ccatttgcgc
     1261 accaggccct cgcctcgttt gatgttctgc gcatcggcga gtttttcttg aatattggga
     1321 atctcgtcgc cttttgtgcc gagggtcatt aggtaactgg taatcgaagc agcctcatcg
     1381 tcggacaggc gcaagctcgg catccgcgtc tcgggcgagt agccgcgcgg atttttaagc
     1441 caatggtata cccattgggg tccgacgact ttagccgcga cgtcttttaa gttcggcaca
     1501 agatccttag attttccgag tggcgtcgta tattcgccgt ccgcaaatcc gtggcagcct
     1561 ttgcagccaa tggattcgac cagcttcttg ccttggccaa ccgagttggc gtcgcctggt
     1621 cggatacctg tgggaagcgg gtgttcctgc gtccacttat cgccttcttc cttggattgc
     1681 gaccagatgt aggacgcaat tgcgatggca tcgctttctt tgagatcgaa gttcggcatg
     1741 cgcgtgcgcg gccgaaactt gtgcgggttc tcgatccagc gcaccatcca gttcggatca
     1801 actttagcgc tgatcttcaa cagactcggt gcgatcttgg gaatattctc gtagcccttg
     1861 acgagatggc agccggtgca gccgacctgt tcgaataatc tctggccttc gaccaatagc
     1921 ggcgcgtcat cggcaaactt ctgcacgtct agatggcacg aagtgcagct cgattgagca
     1981 ctggcgccgc gcagaagcgg gaattcccaa agatgaactt cgccgtgcgc cgcctgggcg
     2041 ctgttgacag ccacgccctg gccttcatga caagtcgtgc agccgaattt ctctggcgaa
     2101 tgagcgttgt cggccaacaa aacttcgcgt cgaggatgcg tcttgaacgg atttgcctct
     2161 ttttcaaagc ccgcccgatt gattgcgaga tggcacgtct ggcaacgatc caccctcgcc
     2221 accggctggt cgaagcgatt gcgatcgaat tcgttgagtg agacttgctg aatttttgga
     2281 atcttataaa acgcaaatgg tccgagcttg aatgtgtcgt tttccatcac gcggacccat
     2341 ttatcccgat ccgcagacat cttgctgagc tcgtcttcga gatctttccc gctcgatttt
     2401 atctttttga tttcttccct gatctggtcg cgccgtgcct gggctttttc cacctcgggg
     2461 tcgagcttcg ccttctctgc gtcaagctcc ttgatttgtt ccaggtaagg cgcgggattt
     2521 cttttttcct ggaccgcgtg atcatgctcg taccacgcct cttccagctc gctcttgatg
     2581 aatttgactt cctggtcttt gtctttgaac gcaaccgtgg ctttggattc ttgctcctct
     2641 agcgcttgaa gctttttggc caagtcaccg cgcgccaagc tggtttggat atcggcgagt
     2701 ttcttgctca gctcttgata ctttgggtcg tcctgtaatt tcttatcctc ttcgtcgtag
     2761 gcggctttag ccttgttgta atcgagtcga taaaaccgcg cctgcacacc cttccatgga
     2821 cggcgcgtaa tgttgtcgtc ccaataggcc cataaagaca acgccaccag cagagccacg
     2881 ccgataacaa agacgctgcc gtaagatttc ttttcttctt ttaagtcgac tcctcgctct
     2941 gccatgccga ctcccttaca cgaccttgcg ttcgagcacg cgtcccaagg aaaaaacccc
     3001 gagtccaatt agcgatacga cgatttcttc tgccattgaa gcgtttctca caatcccggc
     3061 aaacaaggca tagcccatat cggcaagtcc caatgcctgg aggatcttcg ccacgtaaaa
     3121 catctagacg ttaatccaag gggtgacgag aacgtatttg acgttgaaaa gcagccgtaa
     3181 agccattttg gcggggagcg acagcatgag cgcaatcaga accgcagtga tcgagaaacg
     3241 caccatgccc cagcgctcga gaaatgcttt ccctttgacc ttgagcacaa aaaaataaaa
     3301 aagcgcaatg gtcgtgatga aatacatccc gatcatgacc agactgaaca ctgcggacca
     3361 ccagtaatct ctgaatccga ccaggtaagg taggtcgacg ttggtcaacg ccacaacctt
     3421 gtgtggatcc cacttttgcc agggccagaa aagattccac cccggaccgc gaaagaatgt
     3481 accgatcatg atcatcgtga tccacaaaat gtgaaagcct aaaaaaaacg tcaggatttc
     3541 ccatttacgg tctttgaagc aataatagcc attgcctctc gggttgatat cgatgtacgg
     3601 aatcaccatc aacccgacaa taatcagact cggcagcacc actccggcgt tccagggatc
     3661 gaagtaaacc agcatctcct gcagcccaag aaaataccag ggtgccttgg acggattcgg
     3721 cgtgcgcgtc gggttcgccg gctcctccag cggtgcatca acggtcaaag cccacagtgt
     3781 caatgccacg agtacaaaca ag
//
LOCUS       DASULZ010000136         2265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_219510, whole genome shotgun sequence.
ACCESSION   DASULZ010000136 DASULZ010000000
VERSION     DASULZ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219510"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VFY96_02760"
     CDS             complement(<1..570)
                     /locus_tag="VFY96_02760"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX5605405.1"
                     /translation="MDALAIAIVLGMVEGITEFLPVSSTGHLIISGHLLGFVGDAAAS
                     FEVIIQLGAILSVVLLYWRRFVGLIPHPVSGFQASNLQGWSGIWRIAVATFPALLVGY
                     GARHIIKERLFNPTAVTLALAIGGIAILLVERYAAKRKASSLERLTLPQALGVGLFQV
                     LALWPGTSRAAATIVGGMLLGLDRKSAAEF"
     gene            complement(593..>2265)
                     /locus_tag="VFY96_02765"
     CDS             complement(593..>2265)
                     /locus_tag="VFY96_02765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5605406.1"
                     /translation="PWLLTAAVAFVLLYKLGSVALFEPDEGRNAEKAREVLLLNDWIT
                     PHENFYPVLDKPIFFYWLIAFSYKLFGVSEWPARLPSLLSALACVLLVYRFTRAHWGS
                     WVALWSALVLVTTVEFFILARIVIFDMPLTFSETVALLAFYEAAHTEDRRRRAALCLA
                     LYLALGVGTLIKGLVALVIPGIVFFLFILLRGRWQILRRIYLIPGAGVFLAVVLPWYL
                     QAEARNPGYLNYYLWAEHFGRYTTASFDRSEPWYYFIGVALVGFFPWTLVLPWIIKNL
                     WRRSWDDKTLYVVLWVSVPLLFFSASHSKLPHYILPIFPALSILAGATLVGLYEQSGS
                     ELRWAMTSVWVVQSLNALYLLLGLFYPTILAHQIRERFSDMSFLLWLYAIVSLLILAY
                     MASRRITRVRQGPIYLAQVVGLSVFFVLLTEVMISIAPHRSAKQTAMAVLSHLRSGEQ
                     VALFETYMSGVPFYLRAERPVWLITHENKKRTFLGNYYAIGKRHDPVTPWGKAIFNLA
                     DFAEQWKSRTQPFLVIVKDKNLQQLADTIGEAPKRVAQIDEYVLVSNR"
BASE COUNT          644 a          610 c          593 g          418 t
ORIGIN      
        1 gaattccgcg gcgctttttc gatctaaccc gagcagcatt ccgccgacaa tggtcgccgc
       61 ggcgcgcgat gtaccgggcc aaagggcgag aacctggaaa agaccgactc ccaaggcttg
      121 cggaagcgtg agtctttcga gactgctggc ttttcgcttt gcggcataac gctccacgag
      181 aagaattgcg atgccgccaa tcgccagagc taacgtaacg gccgttggat tgaacagtcg
      241 ctctttgatg atgtgacgcg ctccgtagcc gacgagcaac gccggaaacg tcgcaaccgc
      301 aatgcgccaa atacctgacc agccttgcag attcgaggcc tgaaagccgc ttacggggtg
      361 ggggatcaat ccgacaaagc ggcgccaata gagcagcacc acggagagaa tggcgccgag
      421 ctgaatgatc acttcaaagc tcgccgctgc gtcaccgaca aatccgagca gatgacctga
      481 aataatcaag tgtcctgtgg aagaaaccgg cagaaattcg gtaattccct caaccattcc
      541 taaaacgatc gcaatcgcca gagcatccat gccgacctcc tcctacagca tctcaacggt
      601 tggataccaa aacatattcg tcaatctgtg caacgcgctt tggcgcttcg ccgattgtgt
      661 cggccagttg ctgcagattt ttatctttca cgatgacaag aaatggctgc gttcgactct
      721 tccattgctc agcgaaatcg gccaagttga aaatcgcttt gccccatggc gtcacgggat
      781 cgtgccgttt gccaatagca taataatttc ccaaaaacgt ccgcttttta ttctcgtgtg
      841 tgatcagcca aaccggtcgc tcggctcgga gataaaaagg cacccccgac atatacgtct
      901 cgaacaatgc tacttgttcc ccgcttcgca ggtgggacaa cactgccatc gcggtttgct
      961 tcgcggagcg atgcggggct atcgagatca tgacttctgt aagcaacacg aaaaatacgc
     1021 tcaagccgac aacctgggcg agataaatgg ggccttgccg cactcgagta atccgtctgc
     1081 tcgccatata agccagaatc agcaacgaga ctatcgcata gagccaaagc agaaaggaca
     1141 tgtccgagaa ccgctcgcga atctgatgcg ctaaaatcgt tggataaaat aatccgagca
     1201 gcaggtagag cgcattcagg ctttgcacga cccaaactga tgtcatcgcc caacgcaact
     1261 cggatccgga ttgctcatac aagccgacca gtgttgcgcc ggcaagtatg gaaagagcag
     1321 gaaagattgg cagaatatag tgcggcaact tcgaatggga agcggaaaaa aacaacagcg
     1381 gcacggatac ccaaagcacg acatacagtg tcttgtcatc ccaacttctg cgccatagat
     1441 ttttgatgat ccaaggaaga acaagggtcc acgggaaaaa accgaccagg gcaacgccga
     1501 tgaagtaata ccagggctcc gagcgatcga agctggcggt agtatagcgg ccgaaatgct
     1561 ccgcccatag gtaataattc aggtatccgg ggtttcgcgc ttcggcctga aggtaccagg
     1621 ggagaacgac cgcaagaaaa acgccagcgc caggaattaa atagattcgg cgtaagatct
     1681 gccaccgtcc acggagcaaa ataaaaagga agaatacgat tccaggaatg accagcgcga
     1741 ccaagccttt gatgagagtt cccacgccaa gagctagata cagtgccaga caaagagccg
     1801 cgcgccggcg acgatcctcg gtatgcgcgg cttcgtagaa agccaatagc gcgacggttt
     1861 cagagaacgt aagcggcata tcgaagatca cgatccgggc gagaataaaa aattcaacgg
     1921 ttgtcacgag aaccagggcg ctccataaag ccacccaact tccccaatga gctcgagtaa
     1981 aacggtagac caataagaca caagcgaggg cagacaacag cgagggaaga cgggccggcc
     2041 attccgagac gccgaacaat ttataggaaa acgcgatcag ccagtaaaag aaaatgggtt
     2101 tgtccaaaac cgggtaaaaa ttctcatgcg gcgtaatcca atcgttcagt aagagaactt
     2161 cccgagcctt ttcggcgttg cgaccttcgt cgggctcgaa aagtgctacg ctcccgagct
     2221 tatagagcag gacgaaagca accgccgcgg tcaggagcca tggca
//
LOCUS       DASULZ010000137         2595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1234778, whole genome shotgun sequence.
ACCESSION   DASULZ010000137 DASULZ010000000
VERSION     DASULZ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234778"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1477)
                     /locus_tag="VFY96_02770"
     CDS             complement(<1..1477)
                     /locus_tag="VFY96_02770"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX5605407.1"
                     /translation="MISELDTDVLIVGAGPVGLFLANECARRNLRSLIVEERASQSEH
                     SKALAIFPRTMEIFDMAGIVAPFLAAANCVTSVAVMAHDRTLARMEFRPEQSPYSFVS
                     MVPQNVTEKILAAELQKKGGRIDYETTFVSADQEEDSVSAILDHRGNRRTVTASFVVG
                     CDGAHSAIRHLLKISFAGAEYKDSFVLADVDTNDAVPASELLLCPSKFGPVAIFPMSA
                     TRRRIIASIEEPEGDAPSLDLVQRILAQRAPATVKASAMHWSSYFRIHHRHVEKMRVG
                     RIFIAGDAAHIHSPFGGQGMNTGLHDVWNLAWKLELFLRGHGNEQLLDSYNTERLPII
                     EHVIELTHVMTKVLGTPNKLAQALRNTIIPMVSRLAPFQHAFVQRLSELGIAYSNSPI
                     IEGPGERYFDDSMRAGGIKGRFLLMVDKADENRLSDVLKQFSKSFNEIVELRLYDDRG
                     ITLVRPDGYVAFTAHSINDSTLVSLRSLLERQTSRFKISETD"
     gene            1890..>2595
                     /locus_tag="VFY96_02775"
     CDS             1890..>2595
                     /locus_tag="VFY96_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoD-like phosphatase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5605408.1"
                     /translation="MKITRRSFLKTAAAIGASLAWVGPARGSRVQWHERRDLYPQGVA
                     SGDPDPHSVILWTRHPFAQGTRQLLTVEVAEDEAFRRVIAHAQAPVSNAADWTARVLI
                     GGLKPARTYWYRFTDADGNGSRVGRTITAPLPNDPRTVNFAFVSCQDVNEGKLNGYRR
                     MIYEDERAPAAEQLDFVLHLGDFIYEVVEYPDEVKTRYDRTIYEVARIPDGGKSGKLH
                     YPLTVDGYRAVYKGYLA"
BASE COUNT          591 a          716 c          703 g          585 t
ORIGIN      
        1 catctgtctc ggaaattttg aagcggctag tctgccgctc taatagcgag cgcaatgaca
       61 ccagcgttga atcgtttatg ctatgtgctg tgaaggccac gtaaccatcg ggtctgacca
      121 acgtgattcc gcgatcatca tacaagcgca actcaacaat ttcgttaaat gatttcgaga
      181 attgcttcag aacatcactc agacgatttt cgtccgcctt atctaccatc aataagaaac
      241 gtcctttgat tcctccagcg cgcatcgagt catcgaaata ccgctcgccg ggtccttcaa
      301 tgattggact gttagaatag gcgatcccca attccgaaag ccgttggacg aatgcgtgtt
      361 gaaatggcgc aagccgagac accattggta tgatcgtgtt gcgcagagcc tgagccagct
      421 tgttcggcgt tcccaaaact tttgtcatta cgtgcgtaag ctctattacg tgctcgatga
      481 tcggcagacg ctcggtgtta tagctgtcca gcaactgctc attgccgtga ccacgcagaa
      541 aaagctccaa tttccaggcc aggttccaga cgtcgtgtaa gccggtattc atcccctgtc
      601 cgccgaaagg actatgaata tgagcggcgt cgcccgcgat aaagattcgg ccaactctca
      661 tcttctccac gtggcgatga tgaatacgaa agtagctgct ccaatgcata gcactagcct
      721 ttacagtggc cggcgctctt tgcgcgagta tccgctgtac cagatccagt gagggagcat
      781 cgccttccgg ctcttcgatc gaagcgatga tgcggcgtcg cgttgcactc atgggaaaga
      841 tcgccactgg accgaatttg cttggacaca atagcagctc agaggcagga acggcgtcat
      901 tcgtatccac gtcagccagg acgaaggaat ccttgtattc agcaccggca aacgagattt
      961 tcagcagatg ccggatcgcg ctgtgagccc catcacagcc gactacgaac gaggctgtaa
     1021 cggtgcgtcg attacccctg tgatcaagaa ttgcgcttac agagtcttct tcttgatcgg
     1081 cggagacgaa agtcgtttcg taatcgatgc gtccgccttt cttttgtaat tctgcggcga
     1141 gaattttctc ggtcacattc tgcggaacca tggaaacgaa tgaataagga ctctgttcag
     1201 gcctgaattc cattcgggcc agcgttcgat cgtgcgccat tacagccacc gaagtgacgc
     1261 agttggcggc agcgaggaat ggcgctacga tccctgccat atcgaagatc tccatcgtgc
     1321 gcggaaagat tgctagagct ttggagtgtt ccgattgcga ggcgcgttct tcaacgatga
     1381 gcgaacgcaa gtttcgccgg gcacattcat tcgcaaggaa taaacctacc ggaccggcgc
     1441 cgacgatcaa gacatcagta tccagttcgc tgatcatgtt ctgctccttt ccaccgaagc
     1501 gcgagttaca attcaggctg caacctttgc aatgatggcc gcgcgttaaa gtcgaaccac
     1561 ttatctatga cgatgctttg aattgagcag cggatgcccg ttcacatttc gttgtcgatt
     1621 caatcaaggc cgaaccatgt aagtgtcgcc gccccctccg tccagtggcg ggacatgttg
     1681 ttactaagcc agccgaagcg ataccttgac gaccgctggg aggcataaat actggctccg
     1741 cccaaattcg acaagcagta ttaaaggacc tttcgaaggc acgttatcaa gcatgagagg
     1801 gataatatat agttaggcgg ttgtcgtagc gggcgttcgc gtccgaatcg tgaggtggcg
     1861 ccaactgcaa ccggagacgg agtgagacga tgaagattac ccgccgatcc tttctcaaaa
     1921 ctgcagcagc aatcggtgct tcgctcgcct gggttggacc agctcgaggt tcgcgcgtcc
     1981 aatggcatga gcggcgtgat ctctatcccc agggcgtcgc ctcgggcgat cccgatccgc
     2041 acagcgtcat cctctggacg aggcatccgt ttgcccaggg cacacgccag ctcctgaccg
     2101 tcgaggtagc cgaggatgag gcattccggc gagtcattgc gcacgcgcag gcgccggtct
     2161 caaacgccgc ggactggacc gctcgcgttc tgattggcgg actgaagccg gcgcgcactt
     2221 actggtatcg gtttaccgac gccgacggaa acggtagccg cgtcggccgc acgatcactg
     2281 cgccgttgcc aaacgatccg cgcacagtga actttgcatt tgtgagctgt caggatgtca
     2341 atgagggcaa gctcaacggc tatcgccgga tgatttatga agatgaacgt gctcccgccg
     2401 ccgaacagct cgatttcgtt ctgcacctcg gcgattttat ctacgaggtg gtcgagtatc
     2461 ccgacgaggt gaagacacgc tacgatcgca cgatttatga ggtggcgcgc atccccgatg
     2521 ggggaaagag cggcaagctc cactacccgc tgaccgtcga cggctatcgg gccgtgtaca
     2581 agggctatct cgctg
//
LOCUS       DASULZ010000138         5741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1031561, whole genome shotgun sequence.
ACCESSION   DASULZ010000138 DASULZ010000000
VERSION     DASULZ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031561"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1658)
                     /gene="ileS"
                     /locus_tag="VFY96_02780"
     CDS             complement(<1..1658)
                     /gene="ileS"
                     /locus_tag="VFY96_02780"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX5605409.1"
                     /translation="MDYKDTLNLPKTAFPMRANLPQNEPKQVEKWQREKTYFQILDAH
                     ANQPKFILHDGPPYANGNIHIGHALNKILKDVIVKYKAMTGNAAPYVPGWDCHGLPIE
                     LQVEKNVGRAKKVTMTKAEIRRLCREYAEEYVSIQREEFKRLGVLGDWDYPYLTLNPA
                     YEAQEIRELGKIAASGALYRQKKPVYWCASCVTALAEAEVEYEDHTSPSIYVKFAVTD
                     PKGLFPINPTIGTFFVIWTTTPWTLPANQAIAVHPKLLYRLVKTPQGELIISQELIHG
                     VMKALALETTDYEISSGAWMGTELEGVVCRHPWLDRDSNIILGEFVTQDQGTGAVHIA
                     PGHGQEDYEVGLRYGLPVMAPVDAEGKFTAEAGDLQGQSVFKADAHIIQKLVDRGALL
                     KQQTLSHSYPHCWRCKNPVIFRATAQWFVSMEKKNLRQQALDAIDHVRWIPPWGKDRI
                     RGMLESRPDWCISRQRSWGVPIPAVYCRKCNHAILTQELCDHAAAIFEKEGSDSWYTR
                     SLQELIPTRLTCPDCGGRDFLREEDILDVWFDSGVSWAAVVEADP"
     gene            complement(1701..2078)
                     /locus_tag="VFY96_02785"
     CDS             complement(1701..2078)
                     /locus_tag="VFY96_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605410.1"
                     /translation="MRMILPPIRERRLVDRLLSAFFKEYKGADFKKAIAKLCRFYHLR
                     NPKVEWFEYIDWGRTAGKTYENGQIYLVHPENWKKGRKYTSERRWINTVYHEMGHYVF
                     WADAENKADMFAARMVRGVNHHR"
     gene            2321..3319
                     /locus_tag="VFY96_02790"
     CDS             2321..3319
                     /locus_tag="VFY96_02790"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5605411.1"
                     /translation="MGARFTAMLASAATRCGFALGCFAVRVFPRHWLFRLADGLASLG
                     YLLFKGFRAHSTENIQAAFHEQLSETAVENIARRSLRNFFRDCVEIGVVLESSDEEVR
                     DQVPIIGKEYLDAALEKGSGVFVLSAHLGNFFLLGTRLALEGYPVFVLVNQPKDGRFA
                     ELMDRYRLKVKQRTIHARPRRAALRQLNEVLRRNEIAIVIADEYRRGNGVPVPLFGRT
                     VVARRGPATLALRTGAAVVPACVVRQPDDSLQLIIEPELELERSGGTKVDVVENTVRL
                     TRWLESLVRRHPDQWNWMNIRWWQNTNTETLQRQRVERGEKTSMKGETGEKQQEGS"
     gene            3294..4040
                     /locus_tag="VFY96_02795"
     CDS             3294..4040
                     /locus_tag="VFY96_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX5605412.1"
                     /translation="MKSSKKDLDELKKKVALGNRIMFYQGLADYHGHVSARIPGTQRF
                     FIKPVLAPLGEIAPKHIIEVDIDAYMEVCEQNYAQAGNKRAITKLKNPPRETMIHAAI
                     YKTRPDINSVVHTHQTIATAFSVADTPILPIYNQAAVFAPKTPIFPSPRLIYIVQDGK
                     EICQTLGDRTAMLLKGHGIVVCGDSLEYATVHAIYLERTAYMQFIASTVGKPSIMPQS
                     EIDYMKENMTYRSYDAFSYFKSLLPKAARL"
     gene            4074..5666
                     /locus_tag="VFY96_02800"
     CDS             4074..5666
                     /locus_tag="VFY96_02800"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX5605413.1"
                     /translation="MAKREERLTESTSKRPIQDLREWLERVDQMGDLVHVKEPVSCEE
                     EMSAIGYLVAKQKPSPAVLFDNIKGYEKSPFKARSLWNILGPSIPRIALTMEESPETP
                     TVALISRVKDKLKNRMPPREVSASQVQFYENTLTGDQIDLTQLPIPRHWPLDGGRYAG
                     TADCVITRDPDTGYLNVGTYRMMLQGKKETGLYLSPGKDARLHITRSWQQGKAVEVAA
                     AWGIDPLFMVVGSQTFAKNVSEYEFLGGIKGEPIPVVKGKTTNLLLPANAELIVEGVI
                     KPNSVKSEGPFGEFPGYYGRPEAGCPLVEVTAVHYRNNPILTNALMADYPSNEQSGFF
                     SIIRSARIWDDLEKLGIPGIQGVYAHPAGAGGFGMTAIALEQRYAGHAAQALALAAQV
                     PGGAYYTKWIIAVDEDIDPTDMDQVIWAMSSRCNPIDDIDILRNTWSTWLDPTQNPPE
                     KRPYGSKALINACKEHRYLPVFSKRTALRREIYDQVAAEWKKLGLPGQVPAVRAFEEE
                     KKVVYHEVGGFEPGKQPGEEKK"
BASE COUNT         1372 a         1472 c         1603 g         1294 t
ORIGIN      
        1 ggatcggcct caaccactgc tgcccagctt acgccagaat caaaccatac gtcgagaatg
       61 tcctcctcgc gcaaaaaatc ccggccgccg caatcgggac aggtgaggcg cgtcgggatt
      121 aattcctgca gcgatcgggt ataccaggaa tccgacccct ctttttcaaa aatcgccgcc
      181 gcgtgatcgc agagttcctg tgttaggatt gcgtgattac atttgcggca ataaaccgcg
      241 ggaatcggca cgccccatga gcgctggcgc gagatgcacc agtccggccg actttcgagc
      301 atcccacgaa tccgatcctt gccccagggc ggaatccagc gcacgtgatc gatggcgtcg
      361 agtgcttgtt ggcgcaaatt ctttttttcc atcgagacga accactgcgc ggtggcgcga
      421 aagatcaccg ggttcttgca gcgccaacaa tggggatagc tatgggataa ggtttgctgt
      481 ttgagcaagg caccgcgatc gacgagtttc tgaatgatat gcgcatcggc tttgaaaacc
      541 gattgtccct gaagatcgcc agcttcagcg gtaaatttcc cctcggcatc gaccggagcc
      601 ataaccggca atccgtagcg gaggccgact tcgtagtctt cctgaccatg acctggagcg
      661 atatgcaccg cgcctgtacc ttgatcctgg gtaacaaact caccgagaat gatgttcgag
      721 tcacgatcaa gccaaggatg gcggcaaaca acaccttcga gctccgtgcc catccaggcg
      781 cctgacgaga tctcgtaatc agtggtttcg agcgccaatg ccttcatcac accatgaatc
      841 aattcctggc tgatgatgag ctcgccctgg ggcgttttaa ctagtcggta taagagtttc
      901 ggatgaaccg cgatggcttg gttcgcgggc aatgtccaag gtgtggtggt ccagatgaca
      961 aagaatgtgc cgatggttgg attgattgga aaaagcccct tcgggtccgt gacggcaaat
     1021 ttgacataaa tagaaggcga ggtgtgatcc tcgtattcga cttcggcttc ggccaacgcc
     1081 gtgacgcacg acgcgcacca gtagaccggc ttcttttgcc gatatagcgc accggacgcc
     1141 gcaattttcc cgagctcgcg aatctcctga gcctcgtagg ctggatttaa ggtcaaatac
     1201 gggtagtccc aatcaccaag aacaccgagc cgtttgaatt cctcgcgttg gatggaaacg
     1261 tattcttccg catattctct gcacagacgg cgaatctcgg ccttcgtcat ggtcactttt
     1321 tttgctctgc cgacattctt ctcgacctgc aattcgatcg gcagaccgtg gcaatcccaa
     1381 ccgggaacat agggcgcagc gttgcccgtc atcgctttgt atttgacgat aacgtctttc
     1441 agtatcttgt tgagtgcatg gccaatatgg atgttgccgt tggcataggg cggaccgtca
     1501 tgcaaaatga acttcggctg gttcgcgtgc gcgtcgagga tttgaaaata ggttttctcg
     1561 cgctgccatt tctccacctg cttaggctca ttctgaggca gattggcacg catcggaaac
     1621 gccgttttgg gtagattgag tgtatccttg taatccatga cagggcttga ttctggaaac
     1681 cgtgagaatt ggccggctct ttaccggtgg tgattcacgc cgcgaaccat gcgtgctgca
     1741 aacatatccg ccttgttttc cgcgtctgcc caaaaaacat aatggcccat ctcgtggtat
     1801 acggtattga tccagcggcg ctccgaagtg tatttgcgcc cttttttcca gttctccgga
     1861 tgaactaaat aaatctgacc attttcgtag gttttccccg ccgtccggcc ccaatcaatg
     1921 tattcgaacc actcgacttt gggattgcgc aggtggtaaa aacggcacaa cttggcgatg
     1981 gctttcttga aatccgcgcc tttatattcc ttgaaaaatg ccgaaagtaa acggtctacg
     2041 agacggcgtt ccctgatcgg tggcaaaatc attctcatgg ggtgcgtccg gatccactag
     2101 ttgaaaatta gtagcataca accgccacaa tagaaacact cagcgcccag cgacgctcga
     2161 tctgtcgagc tctaagctat tgtaaagtca attgtttctg acgaattatg ctgggccgct
     2221 gcgaattagc ggcctaccaa ccgaagcttg cgatggcagt aaaagtgtca tagattggca
     2281 gccttcaaga cacctgacga gataaaatgt ctgaaaatta atgggggcta ggtttacggc
     2341 gatgctcgcg tcggcggcga cccggtgcgg gtttgcgctc gggtgttttg cggttcgagt
     2401 gttcccgcgc cattggctct tccgattggc tgatggactg gcgagcctcg gataccttct
     2461 gtttaaagga tttcgcgcgc actcgacgga aaacattcaa gcggcttttc atgagcagtt
     2521 gagcgagacc gcggtggaaa atatcgcgcg ccgatcgcta agaaattttt tccgcgactg
     2581 cgttgaaatt ggtgttgtgc tcgaatcatc cgatgaagag gtaagagatc aggttccaat
     2641 tatcgggaag gagtatctcg acgccgccct tgagaagggc tccggagttt tcgttctcag
     2701 cgctcacctg ggaaattttt ttcttctcgg gacacgcctg gcgctcgagg gatatcccgt
     2761 tttcgtgctt gtcaatcagc cgaaggacgg tcgatttgcc gaacttatgg atcgataccg
     2821 attaaaggtg aaacaacgga cgattcacgc ccggccaaga cgagcagcat taagacagct
     2881 caatgaggtt ttgcgtcgaa acgagattgc cattgtgatt gcggacgagt accggagggg
     2941 gaatggtgtt cctgtgccgc tgttcggccg tacggtggtt gcgcgcaggg gcccggcgac
     3001 attggcgctc cgcaccggcg ctgcggtggt gccggcatgc gtcgttcggc aaccggacga
     3061 ttcgctccag ttaatcatcg agcctgagct cgaattggag cgcagcggtg gaacgaaagt
     3121 cgacgtcgtc gagaacacgg tccggctcac gcgctggctt gaaagcttgg tgcgtcgcca
     3181 tccggatcaa tggaattgga tgaatatccg ttggtggcag aatacgaata cagaaacgtt
     3241 gcagcgacag agagtagaac gtggtgaaaa gaccagcatg aaaggggaaa caggtgaaaa
     3301 gcagcaagaa ggatcttgat gagctaaaga aaaaagtcgc cttggggaac cggatcatgt
     3361 tctaccaggg attggccgac tatcacggcc atgtgagcgc gcgcattccc ggcacgcagc
     3421 ggttttttat caagccggtg ctggcaccgc taggtgaaat cgcgcccaaa cacatcatcg
     3481 aagtcgatat cgacgcgtac atggaggttt gtgagcaaaa ttatgctcag gcaggcaaca
     3541 agcgggctat cactaaactg aaaaatccgc cgcgcgaaac tatgattcat gcggcaatct
     3601 ataaaacgag accggacata aattcggttg tgcatacgca tcaaaccatc gccacagcct
     3661 tttctgtggc ggacacgccg attctgccca tctacaatca agccgctgtg ttcgccccga
     3721 aaacgccgat ttttcccagt ccgcgtttga tttacatcgt gcaggacggc aaggaaatct
     3781 gtcagaccct aggagatcga actgcaatgt tgctgaaagg ccatggcata gttgtgtgcg
     3841 gcgatagctt ggaatatgct accgttcacg cgatctacct ggaacgaact gcctacatgc
     3901 agttcatcgc cagtacggtc ggcaagccgt cgatcatgcc gcaatcggaa atcgattaca
     3961 tgaaagaaaa tatgacgtac cgttcgtatg atgcattttc gtatttcaag tcgctgctgc
     4021 ccaaggccgc gcggctctga gctaacccgc aacgactatt ggaggatgaa cttatggcca
     4081 aaagggaaga gagactgacg gagtcgacga gcaaacggcc aattcaagat ctgcgggagt
     4141 ggctcgagcg cgtggaccag atgggcgatt tagttcatgt caaagagcct gtcagctgtg
     4201 aagaggaaat gagcgcgatc ggctatctgg tggcgaagca gaaaccatca ccagcagttc
     4261 ttttcgacaa cataaaagga tacgagaaga gtccgttcaa ggcgcgttcg ctctggaaca
     4321 ttctcggccc gagcattccg cgcattgctt taacgatgga ggaatcgcct gaaacgccga
     4381 ccgtggcttt gattagccgg gtcaaagata aactgaaaaa tcgaatgccg ccgcgcgaag
     4441 tatcggcgtc acaggtgcaa ttctacgaga acactttgac gggagatcag atcgatttaa
     4501 ctcaactgcc catcccgagg cattggcctt tggacggagg ccgatatgcc ggcactgcgg
     4561 actgcgtgat tacgcgcgat cccgatacgg ggtacctgaa cgtcggtacc taccggatga
     4621 tgctccaggg gaagaaggaa accggacttt atctttcgcc gggaaaagac gcgcgcttgc
     4681 acattacgcg atcgtggcag caggggaaag ccgtggaagt tgccgctgcc tggggcattg
     4741 acccgttgtt tatggtggtt ggctcgcaaa cgtttgccaa aaacgtttcc gagtacgaat
     4801 ttctcggcgg tatcaaagga gagccaatcc ccgtcgtgaa aggaaaaact actaatcttc
     4861 tgcttcctgc caatgcagaa ttaatcgttg agggtgtgat caagccgaat tctgtcaaaa
     4921 gcgaagggcc tttcggagag tttcccggct actatggcag gcccgaagcg ggttgtccgc
     4981 tggtcgaagt gaccgctgtg cattatcgca acaatccaat tttgaccaat gcgctgatgg
     5041 ccgattatcc ttcgaacgag cagagcgggt ttttctcgat tatccggtca gctcgcatct
     5101 gggatgattt ggaaaagctc gggatacctg gaatccaggg cgtctatgcg catccggccg
     5161 gtgccggcgg atttggcatg acggccattg ccctcgagca gcgttatgcc gggcacgcgg
     5221 cgcaagcgct ggcattggcg gcgcaggtcc cgggcggtgc ttactacacc aaatggatca
     5281 tcgctgtcga tgaagacatc gacccgaccg atatggatca ggtcatctgg gcgatgagca
     5341 gccgctgcaa ccccatcgat gacatcgata ttctgcgcaa cacgtggagc acttggctcg
     5401 acccgacgca gaatccaccg gaaaagcgcc cgtacggatc caaggcgctg atcaacgcct
     5461 gcaaagagca tcggtacctg ccggtttttt cgaaacggac tgcgctgcgc agggaaatct
     5521 atgatcaagt tgcagcggag tggaaaaaac tcggcttacc tggtcaggtc ccggcggtgc
     5581 gcgcgttcga agaagaaaaa aaggtggtct accacgaggt tggcgggttc gagccgggaa
     5641 aacagccggg agaggaaaag aagtaaatta gaaaagctgg acaccgatta cgagatttag
     5701 tcgccgtcca gcttcttctt aaacgtgacc accaaaaaat t
//
LOCUS       DASULZ010000139         2783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_626359, whole genome shotgun sequence.
ACCESSION   DASULZ010000139 DASULZ010000000
VERSION     DASULZ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626359"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..519)
                     /locus_tag="VFY96_02805"
     CDS             complement(19..519)
                     /locus_tag="VFY96_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605414.1"
                     /translation="MDNVNPHFKPIRVYLESVIVSGMLPTKHQRLSDYFNLTAGYECV
                     ALKDWNAQDFDGHPFAVNSGEFLIYKPEVILVADMRETEKAGSSEVNLERIDKEGRKV
                     MLSVGPFWLQGTIHILPGNTPQQVLTGNTGFVPLTEARLLKPVQSELRTFLINQTKIG
                     VLASLD"
     gene            complement(1331..2155)
                     /locus_tag="VFY96_02810"
     CDS             complement(1331..2155)
                     /locus_tag="VFY96_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX5605415.1"
                     /translation="MARPLRLEFPGAIYHVTSRGNSRQKVFFTDADCELFLRTLSDVV
                     TRYGWICHAYCLMANHYHLLIETPKANLSIGMRQLNGIYTQSFNRRHNRTGHLFQGRF
                     KAILVEKESHLLELCRYIVLNPARVKGSATTSNWQWSSYRSTAGLASVPEFLSTDWIL
                     EQFGKNRLRAQQQYREFVRDGMATRPWDDLKGQIYLGSEAFVEKHASRKKDIKEIPRA
                     QLRAAKPSLERIFARSGKKAIAEAYEQGYRLNEIAAHLDVHYATVSRRLKRIEQTS"
     gene            complement(2305..>2783)
                     /locus_tag="VFY96_02815"
     CDS             complement(2305..>2783)
                     /locus_tag="VFY96_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605416.1"
                     /translation="TGGDLSVELKERWAGSEEMMLNVFDEARDKIAEIMFRVDRDRAG
                     RKFLAVKDQNTLKKYRSRRLMSLMHFFLLHRYKTDLVHYLTPTDDNRLSVRRMIHNGV
                     FREARTDDPNVIAIEVDTARAQKIFANDESIKQFIARQFGALETDSTGRASKPAVA"
BASE COUNT          622 a          779 c          656 g          726 t
ORIGIN      
        1 aattgaattc gtcaaatttt aatccagaga ggctaaaacg cctattttgg tctgattgat
       61 taggaatgtc cgcagttcag attgtaccgg cttgagtagc cttgcctctg tcagaggaac
      121 gaatccagta ttgcccgtca agacctgttg gggagtattc ccgggtaaga tgtgaatcgt
      181 tccttggagc caaaaggggc cgacactcag cataactttt ctgccttctt tatcgatccg
      241 ctcaagattc acttctgatg aaccggcttt ctctgtttcg cgcatgtcgg cgaccaatat
      301 cacttccggt ttgtatatca gaaattcccc agagttgacc gcaaagggat ggccgtcgaa
      361 atcttgcgcg ttccagtctt tgagagcgac gcattcgtag ccggccgtca aattaaaata
      421 gtcgcttagt cgctggtgct tggtaggaag catcccactg acgatcaccg actcaagata
      481 aactcttatc ggtttaaaat gtggattaac gttgtccatg gttgcccttt cgtctacatc
      541 ggcagtatct cccttgcgct tatcacaaga ccgcgagctt tcgaatcatt ctgcggcgac
      601 cgccgtggca ttccctttaa agccatctca gagatcaatc ccaattttcg cgaaagtacc
      661 ggagcccgtt ccggttaagc tcttaaacga tgccttttac gctacctgat gctgtgaaac
      721 aaataacttc ctttggtccg accccaagac gccgacaatt ccgcgaatta aaatccgtaa
      781 tgcatgcgcc accgcaacct tgagtctcag ccagctgaat agagattcga cccctttcag
      841 tcggcgagat tttgtcgggg agcttgtcga acggttgaac gacttgaacg tcttgaacaa
      901 acttttggtg ccagcggtta gtgatatgtc taccctgctt ttgctcctta atcttctgtt
      961 ccagccaaag attaacgagc gtatccgacg acacgccttg cttttggcca ccgactggac
     1021 cctcgccgat aaatctcttt caaccgggta gtaagtggct tccggctcgg ccacgacttc
     1081 gaacttcacc ttttagtctt tccccacata tcgcttaggt cgtgtttatc ccaatattct
     1141 ccaagctgcg catacgaccg agctctagag atggaactct taagtttttt catagcgctt
     1201 cctttcactt caaccgccat ctccggtcag tgatcctatc atttcgcgcc ctgtgcggct
     1261 aactttcgcc ggtccacagc gactccgtct tctcgggcgc gctttcaatg gaaaaagtag
     1321 ccgtgatact ctaactcgtt tgctcgatcc gtttgagtcg gcggctcacc gtcgcatagt
     1381 ggacatccaa atgcgcggcg atttcgttta gccgatagcc ctgctcgtac gcctcggcaa
     1441 tcgctttttt cccgctcctc gcaaatatcc tctccagcga cggcttcgca gctcttaatt
     1501 gcgctcgcgg tatctcttta atgtcctttt tacgagaagc gtgtttttcg acgaacgctt
     1561 cactccccaa atagatctga cccttgagat cgtcccaggg ccgagtcgcc attccgtccc
     1621 taacgaactc ccggtactgc tgctgcgctc gtaaccggtt cttgccaaat tgctccagaa
     1681 tccagtcggt gctcaaaaac tccggcacag aagccagtcc ggccgtcgac cggtaactac
     1741 tccattgcca atttgaagtc gtagcactgc ctttgacccg agcgggattg agcacgatgt
     1801 agcggcaaag ctccagcagg tgtgattctt tttcaaccag aatcgccttg aaccggccct
     1861 gaaacagatg tccagtccgg ttatgccggc ggttaaaact ttgggtgtaa atgccgttga
     1921 gctggcgcat gccgatcgag aggttggcct taggggtctc gatcagcaga tgataatgat
     1981 tggccatcag gcagtaggcg tgacagatcc aaccgtaccg agtgacaaca tccgacagag
     2041 tgcgcagaaa cagctcgcaa tcagcgtcgg taaaaaatac tttttggcga gaattgccgc
     2101 ggctggtcac atgatagatc gcaccgggaa attctaatcg tagagggcgc gccatgacgt
     2161 tccgtaattt atccgaccaa atgtgtcatt gcaagacctg accccaatgt gacccacgtc
     2221 caattgaccc taatgacaaa ccttcttttc tcctcacgag aggattctcc ttcccgacaa
     2281 tgtcagcacc tcggggactg agcgttatgc caccgcaggc ttgctcgcgc ggccggttga
     2341 atcagtctcc aaagctccaa actgtcgagc gataaactgc ttgatcgatt catcgttggc
     2401 aaagatcttc tgggcgcgag ccgtatcgac ttcaatggcg atcacattcg gatcgtcggt
     2461 ccgggcctct cggaacacgc cgttgtgaat catacgccgg acggaaagcc gattgtcgtc
     2521 cgtcggggtt aagtagtgca cgagatccgt cttgtatcga tgcaggagaa aaaagtgcat
     2581 caggctcatc agacgccgag aacggtattt tttgagcgtg ttctggtctt tgacggcgag
     2641 aaattttcgc cccgcccggt cccgatcgac tctaaacatg atctcggcta ttttgtcgcg
     2701 ggcttcgtcg aaaacattga gcatcatctc ttcggacccc gcccatctct ccttcagctc
     2761 gacgctcaaa tcaccgccgg tgt
//
LOCUS       DASULZ010000140         2020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_18003, whole genome shotgun sequence.
ACCESSION   DASULZ010000140 DASULZ010000000
VERSION     DASULZ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18003"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VFY96_02820"
     CDS             complement(<1..511)
                     /locus_tag="VFY96_02820"
                     /inference="COORDINATES: protein motif:HMM:NF037004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605417.1"
                     /translation="MSVGRLLEEGHYTRAKLNEDVSKKFLQTYLEMLDFSHLFFTQED
                     VDGITAKYANSMAGDVLMGTLKPGYEIYALYTKRVDERVAKIKELLKQPMDFKGNATV
                     ELSRQKSPWPKNEAEADQLWRGRIANELLQEHLSEHPIEPAPQLVSRRYERLAKNVHE
                     QDKDEQMKLY"
     gene            739..1359
                     /locus_tag="VFY96_02825"
     CDS             739..1359
                     /locus_tag="VFY96_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5605418.1"
                     /translation="MAPATYKSFCGGIFETNCYLFEAPEGWILFDAPDGACEWLRSLN
                     IHPKLLLLTHGHVDHIQDVARIKQQFHCQIGCHPLTAPMISDRDFFRNFGFALEVEPV
                     EPDFLIEETPNRNFLGADFQVLDVPGHCPGSLCFFSRNDRLLIGGDVLFAGSIGRGDL
                     PGGDMDLLIGGIRNKLFPLDDDVMVLSGHGRPTTIGQERLTNPFLQ"
     gene            complement(1552..>2020)
                     /locus_tag="VFY96_02830"
     CDS             complement(1552..>2020)
                     /locus_tag="VFY96_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5605419.1"
                     /translation="EDHLDVLVNNAGIIADGDDAILEISDDLFRKTLETNTLGPLRVT
                     QAFMPVLKKSKAPRVINVSSGGGQLTGGADGWAPAYCISKTALNGVTVQLAAALPKFA
                     VNSVCPGWVRTEMGGENASRSVEEGADTIVWLALEAPQNLSGKFLRDRKEIPW"
BASE COUNT          426 a          580 c          544 g          470 t
ORIGIN      
        1 gataaagctt catctgctcg tccttatcct gctcgtgaac gttcttggcc agacgctcat
       61 accggcgaga gaccagctgc ggcgccggct cgatcgggtg ctcgctcaga tgttcctgaa
      121 gcagttcgtt tgcaattcgg ccgcgccaga gttggtcggc ttcagcctca ttttttggcc
      181 agggcgattt ttgacggctc aattccaccg tcgcattgcc cttgaagtcc atcggctgtt
      241 tcaaaagctc tttgattttc gccacacgct cgtccacgcg ctttgtgtat agcgcgtaaa
      301 tctcgtaacc gggcttcagc gttcccatga gaacgtctcc cgccatggag ttggcgtact
      361 tcgcagtgat cccatcaacg tcttcctgcg tgaaaaagag atggctgaaa tccagcatct
      421 cgagatacgt ctgcaggaat ttcttcgaaa catcttcgtt cagttttgcc cgggtgtaat
      481 gcccctcctc caggagtcgc ccgacggaca tcgccacggt ttctctcgaa ctcgccggtg
      541 cgggcggagc cgcaaacgcg gctgtaaaaa ggacggtgca aagggcaagt gatcgccgaa
      601 aagatggtct cattggtaaa atggaggttc aagacattcg cagaattcgc cacgcagcgc
      661 aagacaacga gacaaagtcg gcattattgg gtagagatag acaatcgaca gcgcggaaca
      721 ttcaaaaact tcattacgat ggcacctgcg acttacaaat cgttttgcgg agggattttc
      781 gaaaccaatt gctacctgtt tgaagcgccg gagggatgga tcttgttcga cgcacctgat
      841 ggtgcgtgtg aatggctccg ttccctgaat atccatccca aacttttgtt gctgacacac
      901 gggcacgtcg atcacatcca ggacgtcgcc agaattaaac aacagtttca ttgccagatc
      961 ggatgtcacc cgctcaccgc gcctatgatt tccgatcgcg atttctttcg gaacttcgga
     1021 ttcgcgctgg aagtcgaacc ggtcgagcca gattttctga tcgaggagac gccaaaccgg
     1081 aactttctcg gtgcggactt ccaggtcctg gacgttcctg gtcattgccc gggcagtctt
     1141 tgcttctttt cgcggaacga ccggttgctc atcggtggcg acgtcctttt cgccggcagc
     1201 atcggccggg gtgatttgcc cggcggcgat atggatctgc taatcggcgg gatcaggaac
     1261 aagttatttc cgctggacga tgacgtgatg gtgctgtccg gccacgggcg gcccactacg
     1321 atcggccagg aacggctgac aaatccgttc cttcaataaa gatgcggcgc gcacggagtg
     1381 cgcgccctag caagtatcac aaacgcagaa aagtacggcc cttcgcgaga aatcggatac
     1441 cgcgtggcac ggttttgcgc ccgcggggct ttcgggatag gaatgaaagc cgtgccagga
     1501 cgcaccggat tcctaaagtg ttgcgcgcga aattcaatgc tcttgtttga atcaccaggg
     1561 aatctctttg cggtcgcgca gaaatttacc actgagattc tgcggcgctt caagggcgag
     1621 ccacacaatc gtgtccgcgc cttcctccac tgagcggctg gcgttttcac cgcccatttc
     1681 cgtcctcacc caacccgggc acaccgaatt gacggcgaat ttcggtagcg ccgctgccag
     1741 ctgcacagtt acgccattca gcgcagtctt cgaaatgcag taagccggcg cccagccatc
     1801 cgcgccgcca gtcagttgac cgccgccgct cgagacattg atcacgcgcg gcgctttgct
     1861 tttcttgagc accggcatga acgcctgcgt tacgcgcagc ggacccagcg tatttgtttc
     1921 cagagtcttt cggaacagat cgtcgctgat ttctaaaatt gcatcatcac cgtcggcaat
     1981 gatccccgcg ttgttcacga gcacatcgag atgatcctcg
//
LOCUS       DASULZ010000141         3563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1337120, whole genome shotgun sequence.
ACCESSION   DASULZ010000141 DASULZ010000000
VERSION     DASULZ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337120"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..695)
                     /locus_tag="VFY96_02835"
     CDS             complement(204..695)
                     /locus_tag="VFY96_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX5605420.1"
                     /translation="MESILIVYHSKTGGTYQMADAAARGAASEPEIGIDFTHAADAGP
                     DNLLQSSAYIFATPENLAMMSGVMKDFFDRTYYSVLDRIVGRPYATLVCAGSDGENAA
                     RQIQRIAVGWRLKAIADPIIVCTHAQTPDAILAPKQIGEFDLKRCEEVGATVAAGLAL
                     GIY"
     gene            complement(856..1578)
                     /locus_tag="VFY96_02840"
     CDS             complement(856..1578)
                     /locus_tag="VFY96_02840"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605421.1"
                     /translation="MPIKIGFLGSSAPSSPHHDSFRALIPQEISFDFVQESRAKASLY
                     DARGKLVSLIQQAQELIRENGWDGIIISGAPKEALNPGMWQRVCQEISIPVALALRSS
                     VAALKVFGAKKILLMSPVDDELKRLYCAYLRDFEIDALALPQTLRAHTDAQRLTSKDV
                     ESMTRDSLAKLSDVDAIYFQGALLDPLPVLQKMEAELGLPIIASNPAMLWVILSKLGQ
                     RYRMAGYGKLLSDWPALPSGSF"
     gene            complement(1602..1796)
                     /locus_tag="VFY96_02845"
     CDS             complement(1602..1796)
                     /locus_tag="VFY96_02845"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEX5605422.1"
                     /translation="MPVVHIYMYAGRSKEQKSEMVRRISKDFEEVLNVKPESLNVLFH
                     DMEKSDWGIRGALASETPSK"
     gene            2097..3092
                     /locus_tag="VFY96_02850"
     CDS             2097..3092
                     /locus_tag="VFY96_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605423.1"
                     /translation="MSLIRLSALAFLGFTLSLLVPPVGADELNVYFKTTPGVERLRPY
                     ADPASMSLLVTDANGRPLTEGSVALRLDAPRSGVFFSTDFPWVEGTHLQEMQLDLRQG
                     RANWKYLLPIRGEYRLAVNVITADGRKASKEFTFNVRENEKKWGALAAFSAGLFCLGF
                     TAGRVFTAAPKEAVIVLLALLSAFGGRADSSSTQSDASAALEVAPATVGRPTLVRWRF
                     NDNVDPVSARVLLSLAIVHLEKGKPVFEVERIPVPGEFAMNFQFTDGAEYRLVAVGEK
                     PGRPPVRSEKVVAAAALEPPAKAMIPAFGYFIGLIAIGLALGRWSKRREGVKGEE"
     gene            complement(3141..>3563)
                     /locus_tag="VFY96_02855"
     CDS             complement(3141..>3563)
                     /locus_tag="VFY96_02855"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5605424.1"
                     /translation="EKVEERKRRIGHFALRCVEPDQCAEFYREVFELTEMEKPSGDPN
                     HYLSDGRVTLAIMPWKIADFYGSGIERPALDHIGFKVESMAALKADLEKSVHLRTQED
                     RPEKNPEREVRQKLLATCCFGQLQLSDPDGVLIDVSEN"
BASE COUNT          765 a          966 c          985 g          847 t
ORIGIN      
        1 gaatgcgggt caggagtgcc ctttctcctt cgcggtcatg acagtgtcgc aagcgaaagc
       61 catgctgagc gatagagaag catctcgatt tcgcagccga tttgtcatat cagatcttcg
      121 gtttcgcctc gcaggatcac agttagcacg tagtgtcggc cggacgaaag gactccggcg
      181 tcaagtgaag ccggatttgt cacctaatat attcccagcg ccaaaccagc tgcaacggtg
      241 gcgccgacct cctcgcaacg tttgagatcg aattcgccga tctgttttgg cgcgagaatc
      301 gcgtcaggcg tctgtgcgtg cgtgcagaca ataataggat cggcaatcgc tttaagccgc
      361 cagccgaccg caatgcgctg aatctgacga gcggcgtttt ctccgtcgct gccggcgcaa
      421 accagcgtcg catacggtct gccgacgatc cgatcgagga cggaatagta agttcgatcg
      481 aagaaatctt tcataacgcc tgacatcatt gcgagattct ccggagtcgc gaaaatgtaa
      541 gcggatgatt gaagtaaatt gtcagggccg gcatcagccg catgagtaaa atcgataccg
      601 atctcgggct cgcttgcggc tcctcttgcc gcggcgtcgg ccatttgata ggtgccgccg
      661 gttttggagt gataaacgat caaaatgctt tccatcgcac accattcaaa tacagcggaa
      721 cgaacggcca aaccagtgat cgagacgatt tgtcatttcg agcgcgcagc gagaaatcct
      781 tcttctgcgc agatccattc ctgctaacag gcgagagaaa gattttctcc cagcactttg
      841 cgttagagga tctagctaaa acgatccgga cggcagcgcc ggccagtcgg agagcagttt
      901 tccgtagccg gccatacgat aacgctgccc aagtttcgac aaaatcaccc aaagcatcgc
      961 cggattgctg gcaataattg gcaggcccag ctcagcttcc attttttgca acaccggcag
     1021 cggatcgagc agtgcgcctt gaaaatagat tgcatcgacg tcggaaagtt tcgccaagct
     1081 gtcccgagtc atcgactcaa cgtccttgga cgtgagccgt tgcgcgtcgg tgtgagcccg
     1141 cagagtttgc ggcaaagcga gggcgtcaat ttcgaagtca cgaagatagg cgcaatagag
     1201 ccgtttgagc tcgtcgtcga ccgggctcat caacaaaatc ttttttgctc cgaaaacttt
     1261 gagggcggca accgacgacc tgagcgccag cgccaccggg atgctgattt cttgacacac
     1321 ccgttgccac attcccggat tgagagcttc tttgggggca ccgctgatga tgatgccatc
     1381 ccaaccattc tcccggatca gctcctgggc ttgctggatc agcgaaacaa gcttgccgcg
     1441 agcatcataa agggaggctt tcgctctgct ctcctgtacg aagtcaaatg agatctcctg
     1501 agggatcaat gctctgaagc tgtcgtggtg tggactggag ggagcgctcg atccgaggaa
     1561 accaatcttg atgggcatca atttttccgg cttcgagctt gtcacttcga cggcgtttct
     1621 gaagccaggg caccacgaat gccccaatcg cttttctcca tgtcatgaaa caacacgttc
     1681 agcgactcgg gtttgacgtt cagaacctct tcgaaatcct tcgaaattct tctgaccatt
     1741 tcactttttt gttccttgct tcgccctgca tacatataga tatgaacgac cggcattttg
     1801 acctcctgaa aagacttgct ctcatccata cgaaaggctc cggcgaaagg caagccgttg
     1861 caatccttga cagcagcagc cgatcgtatg attatgaagc catcggagga gcgcaccgtg
     1921 gaaagaatcg ttcatgcttg gctcggttta gctgtttcag aacaggcttc atttttgatc
     1981 aagtccggaa tcttcttatc cgtctgtggc gccactctat tctttgctct gggtgcgagt
     2041 taccccgcgg ttcacgatac gctgcacaac gttcgtcacg ccttggccgt tgttccttgt
     2101 cattgatcag gctcagcgcg ttagcgtttc tcggattcac tctttcgctc ttagtcccgc
     2161 ccgttggggc cgacgagctc aacgtttatt ttaaaaccac accgggggtc gaacggcttc
     2221 gtccctacgc cgatcccgcg agcatgtcgc tgctcgtaac cgacgccaac gggcgaccgc
     2281 tgacggaggg atctgtggcg ctccggctcg atgctccgcg atccggcgtt tttttttcca
     2341 ccgattttcc ttgggttgaa ggtacgcacc tgcaggagat gcagctcgat ttgcggcaag
     2401 gccgcgccaa ctggaaatat cttttgccga tccggggcga gtatcggctc gcggtaaatg
     2461 tcatcacggc ggacggccgc aaagcgagca aagaatttac tttcaatgtg cgtgagaatg
     2521 aaaagaagtg gggcgcgttg gcggcgtttt cggctggttt gttttgtctc ggttttacgg
     2581 cgggaagagt attcaccgcc gcgccgaagg aagccgtaat cgtcctgctc gcgctgctct
     2641 cggcgttcgg aggtcgcgcc gatagtagct ccacgcaatc cgacgcaagc gcggctttgg
     2701 aagtagcacc ggcaactgtc gggcggccaa cactcgtgcg gtggcgattt aatgataacg
     2761 tcgatccagt atcggcgagg gttttgctat cgctcgcaat cgttcacttg gaaaaaggca
     2821 aaccagtttt tgaggtcgaa aggattcccg tcccagggga gttcgcgatg aattttcagt
     2881 tcaccgacgg cgcagagtac cgtttggtgg ccgttggcga aaaaccggga agaccgccgg
     2941 tacgcagcga gaaggttgtt gccgcggccg cgttggagcc tccggccaaa gcgatgattc
     3001 cggcgttcgg ctattttatc ggtctaatcg cgatcggttt ggcgctcggc cgatggagca
     3061 aacgccgaga gggcgtgaag ggtgaggagt gaagagtgaa agcggattcc actcacttct
     3121 ttcccctcct tgtaccgggt ttagttttcc gagacgtcga tcaagacgcc gtcaggatca
     3181 gatagctgga gctggccgaa gcagcaggtg gcgaggagtt tttgcctgac ttcgcgctcg
     3241 ggatttttct ccgggcgatc ttcctgggta cgcagatgca cgctcttttc caggtcagct
     3301 tttaaagcgg ccatgctttc cactttgaat ccgatatgat cgagcgccgg gcgttcgatt
     3361 cccgagccgt aaaaatccgc gatcttccac ggcataatcg cgagagtgac tctgccgtcc
     3421 gatagatagt ggttgggatc gccggaaggc ttctccattt cagttagttc gaacacttca
     3481 cggtaaaatt ctgcgcattg atcgggctcg acacagcgca acgcaaaatg accgatgcgc
     3541 cgtttgcgct cctctacttt ctc
//
LOCUS       DASULZ010000142         3278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1337165, whole genome shotgun sequence.
ACCESSION   DASULZ010000142 DASULZ010000000
VERSION     DASULZ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337165"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VFY96_02860"
     CDS             complement(<1..392)
                     /locus_tag="VFY96_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5605425.1"
                     /translation="MKPEVAKAVVFGLLAVSILAGLIVLGSRNLSHFDAALVGYTFAV
                     LFAAFGITYRYAMWLQRPPTALYWKRGWQVFFRPGRIGRNAGNWLRRVSSDVALNRFI
                     FRRGAERWLAHWLIMWGCVIAGAITFPLV"
     gene            complement(480..902)
                     /locus_tag="VFY96_02865"
     CDS             complement(480..902)
                     /locus_tag="VFY96_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5605426.1"
                     /translation="MTEPRKILTIRTLMAQNPATLDRNETLDIPDTVMNLGRIRHMPV
                     VEDGKVVGILSQRDLFRSALIVALGFGRKTTNALIKTIRVKEVMTEPAITIAPEAAVS
                     DAARLMIEKKIGCLPVVQNEKLVGLITETDILRYVMEC"
     gene            complement(963..2168)
                     /locus_tag="VFY96_02870"
     CDS             complement(963..2168)
                     /locus_tag="VFY96_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5605427.1"
                     /translation="MTLRIALRALARNKLRAFLTMLGIIIGVGAVIAMVAIGEGAKST
                     IRAQIAALGTNVLIVLPGSNVQGGVRAGFGNVNTLIDADAKAMSRELPSVAFVSPVLR
                     RQEQVVAANLNWGTLAQGVAPEFQQIRDWEVAQGRFLHEGDMDSAAKVAVIGQTVARQ
                     LFGSDDALDAVIRIRNIPFRVIGVLAPKGQTGQGTDQDDTVMIPYSTMQKRLMRITWV
                     QSIVVKAVSAERVEEAEEQITLLLRQRHRIGPDREDDFNVRNLSDIAEAATTTARVMA
                     VLLGSVASISLLVGGIGIMNIMLVSVTERTREIGIRMAVGARSRDIMLQFLVEAVVMA
                     AMGGLIGILLGIGSSEAIKQWAQWPTLINPGIIAIAFLFSGAVGVFFGFYPAKKAANL
                     DPIEALRYE"
     gene            complement(2165..2896)
                     /locus_tag="VFY96_02875"
     CDS             complement(2165..2896)
                     /locus_tag="VFY96_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5605428.1"
                     /translation="MSELIRVEEVHKIYRMGDVEVPALRGINLTIAHGEFVAVMGSSG
                     SGKSTFMNILGCLDRPSTGRYFLEGDEVGSLSSDEWAYIRNKRIGFVFQGFNLLSRTS
                     ALENVELPMMYNNGVASQERHRRAIEVLTLVGLGNRLDHTPNQLSGGQQQRVAIARAL
                     VNRPPLILADEPTGNLDSATSNEIMGLFEQLNRNQHISVILVTHESDIAAHARRQIVF
                     RDGLVVSDQRNEKVCAEWSGECRRS"
     gene            complement(2893..>3278)
                     /locus_tag="VFY96_02880"
     CDS             complement(2893..>3278)
                     /locus_tag="VFY96_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605429.1"
                     /translation="DVLTIPNMALRFKLPEEKNESQELLRQEQSRTAAPRVGARRTSR
                     TGGAGSSAAARGRSVKVYLLRDGRPRPIEVQVGITDGSRTEVIAGELKENDPVIIGMS
                     SSATGQTSAAPVNPFQPPAPRPGFR"
BASE COUNT          718 a          983 c          846 g          731 t
ORIGIN      
        1 accaagggaa acgtgatggc accggcgatg acacagcccc acatgatgag ccaatgcgcc
       61 aaccagcgtt ccgcgcctcg gcgaaatata aatcgattaa gcgccacgtc gctgctgacg
      121 cgtcgaagcc aattgccggc gttgcgtccg atccggcctg gtctgaagaa tacctgccag
      181 cctcttttcc agtagagtgc ggtgggcggt cgctgcagcc acatcgcgta acgataggtg
      241 atcccgaagg ccgcgaaaag aacggcgaac gtataaccca ccaatgcggc atcgaaatgg
      301 gacaaattgc gtgatccgag gacgatcaga cccgccaaaa tacttactgc taacaggcca
      361 aagaccaccg ctttagccac ttcaggtttc attagccgtt tcatcatttc accacagcgc
      421 tagcgcgcga tttccgaggg ttatcgtgca acaaaaaggt tttgacgtct acttttcagt
      481 tagcactcca tcacgtagcg cagaatatcg gtttccgtga tcaatccaac aagtttttca
      541 ttttgaacca ccggcagaca gccgattttc ttttcgatca tcaggcgcgc ggcgtcgctg
      601 accgctgcct ccggtgcaat cgtgattgct ggttccgtca tgacttcctt gactctgatg
      661 gtcttgatca gcgcattggt tgtttttcgg ccaaatccca gcgccacgat caatgccgaa
      721 cgaaacaaat cacgctggct gagaatgccc acgaccttgc cgtcttccac cacaggcatg
      781 tggcgaatgc ggccgagatt catgacggta tcggggatat cgagcgtttc gttgcggtct
      841 aacgtcgccg gattctgtgc catgagagtt ctgattgtca gaatttttct tggttccgtc
      901 atttgttggt cctttccgaa cgctaccgct gacaagtcgg cataattata cttgcgaaat
      961 gcctattcgt atctgagggc ttcgatgggg tccaagttcg ccgctttctt ggccggataa
     1021 aatccgaaga agacacccac cgctcccgag aagagaaatg cgatcgcaat aattcccgga
     1081 ttgatcaagg ttggccactg tgcccattgc ttgatagctt cggagctgcc aataccaagt
     1141 agaattccga tcaggccgcc catcgccgcc atcaccaccg cttcgacaag aaactgcagc
     1201 ataatgtcac ggctgcgcgc cccgacggcc atgcgaatac caatttcgcg ggtcctctcg
     1261 gtcaccgaca ccagcatgat attcatgatg ccaataccac ccaccaggag cgaaatagaa
     1321 gccacgctgc cgagcagcac tgccatcacc cgcgccgtag tcgttgcggc ctccgcaata
     1381 tcggacagat tgcgcacatt gaaatcgtct tcgcggtccg gaccgatgcg gtggcgctgc
     1441 cggagcagca aagtgatttg ctcctcggct tcttcgactc tttcggcgct caccgccttc
     1501 accacgatgc tttgcaccca ggtgatccgc attaaacgct tctgcatcgt cgagtagggg
     1561 atcatcaccg tatcatcctg atccgttcct tgtccggtct ggcctttggg cgcgagcacg
     1621 ccgatgacgc gaaatggaat atttcggatg cgaataacgg catcgagagc atcgtcactg
     1681 ccaaaaagtt ggcgcgcgac ggtttgaccg atcacggcaa ctttagccgc actatccatg
     1741 tcgccttcat gaaggaaacg tccctgagca acctcccaat cgcggatctg ttgaaattcg
     1801 ggggctacgc cctgagccaa agtcccccag ttcaaattgg cggcgaccac ctgttcctgg
     1861 cgccgaagca cgggcgagac aaacgccact gagggcaatt cgcgcgacat tgccttggca
     1921 tcggcatcaa tcaatgtatt cacattgccg aagccagccc tgactccgcc ttgaacattg
     1981 ctgcccggca acacgatcaa gacattggtg ccaagagcag cgatctgcgc gcgaatagtc
     2041 gatttggctc cttcgccaat cgccaccatc gcgatcacgg cgcccacgcc gatgatgatg
     2101 ccgagcatgg tcaaaaaagc gcggagctta ttgcgtgcca gcgctctgag cgcaatcctc
     2161 aatgtcatga gcgcctgcat tcaccgctcc actcggcaca caccttttca ttccgctgat
     2221 cagagacgac gagcccgtcg cgaaacacga tttgtcgcct agcgtgtgcc gcgatgtcgc
     2281 tttcgtgcgt cacgagaatc acagagatat gctgatttct gttgagttgc tcgaacaagc
     2341 ccatgatctc attgcttgtc gctgaatcga ggttacctgt gggttcatcc gccaaaatga
     2401 gcggcgggcg attgaccaaa gctcgcgcaa tcgcaacacg ctgctgttgt ccgcccgaga
     2461 gttgattagg ggtatggtcg agtcgattac ccagcccgac cagggtaaga acctcgatcg
     2521 cgcggcgatg cctttcctgg ctagcgacgc cgttgttgta catcatcggc aactcgacgt
     2581 tctccagagc gctggtgcgc gataacagat tgaagccttg gaatacaaag ccgattcgct
     2641 tattgcgaat gtatgcccat tcatcagaac tgagcgagcc aacttcgtct ccttctaaga
     2701 aataccggcc cgtgctcggt cggtcgagac aacccaagat attcatgaag gtcgatttgc
     2761 cagatccgga tgagcccatg actgcgacaa actcgccatg ggcgatcgtg agattgatgc
     2821 cgcggagggc gggcacctcg acatcgccca tgcgatagat cttgtgcact tcctccaccc
     2881 gtatgagctc gctcatcgaa agcccggacg aggagccggc ggctgaaatg ggttcactgg
     2941 ggcggctgaa gtttgccccg tagcgctgct cgacattcca atgatgaccg gatcattttc
     3001 cttaagctcg ccggcgatga cttcggttct tgatccgtcg gtaatcccga cttgcacttc
     3061 tatcggccgc ggcctaccgt ctcgcagcaa ataaactttg accgaacggc ctcgcgccgc
     3121 tgcgctgctt cccgcaccac ccgtccggct ggtccgccga gcgccgacac gaggagccgc
     3181 cgtccggctt tgttcctgtc gcagcagttc ctggctctca ttcttttctt ccggtagctt
     3241 gaagcgcagc gccatattcg gaatcgtcag cacgtctt
//
LOCUS       DASULZ010000143         2313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1031988, whole genome shotgun sequence.
ACCESSION   DASULZ010000143 DASULZ010000000
VERSION     DASULZ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031988"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VFY96_02885"
     CDS             <1..322
                     /locus_tag="VFY96_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605430.1"
                     /translation="IFVSQEDKMHFADKRTGYALCRARLAQRRVVVVRTLQHIASERN
                     NLETIRGTRIRPALRKRRGLLDKVYGWYDAELRRIDSVLLRLNALSIGQQVQTHHKSF
                     RASA"
     gene            533..2047
                     /gene="gndA"
                     /locus_tag="VFY96_02890"
     CDS             533..2047
                     /gene="gndA"
                     /locus_tag="VFY96_02890"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014436786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEX5605431.1"
                     /translation="MVEKTLADVGLIGLAVMGQNLALNMADHGFNVAVFNRTTQVMKD
                     FIAANPNTPGGLVGCEKLSELMAAVKKPRKIILLVKAGPAVDTVAKQLFEAGLEADDL
                     IVDCGNSQWTDTIRREKEYSARCRFFGSGVSGGEVGARFGPSLMPGGDRQAWKHLEPI
                     WRAIAAKVDPKSGKPIETARPGRPVEGGVPCAAYIGENGAGHYVKMVHNGIEYIDMQL
                     IGEAYFLIRNLLNVRPNDLAEIFAQWDEGDLDSYLIQITADILKQRDPRHPDGFLVDY
                     VLDTAQQKGTGKWTSINALDLGIPANSIAEAVFARCLSALKEERVQASKHLRGPDYSY
                     DGKRDELIDSVRDALYCSKICAYAQGFQLMRAAEVEYGWKLDLGTIASIWRGGCIIRA
                     RFLQKIADAYLQDPRLGNLLLAPYFQEQIHQNQKRWRKVVTLAAESGTPAPAFMSALA
                     YYDGYRTANLPANLLQAQRDYFGAHTYKRTDESPGKSFHLDWPKADRPEIELSS"
     gene            2102..>2313
                     /locus_tag="VFY96_02895"
     CDS             2102..>2313
                     /locus_tag="VFY96_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605432.1"
                     /translation="MLIFLLATFLAGCSTASNYDAMQADVDQAAAVVERFQAIPEKGI
                     PAAVMRAARGLAILTVTKAGFIGSVR"
BASE COUNT          530 a          570 c          688 g          525 t
ORIGIN      
        1 gatttttgtt tcacaggagg acaaaatgca ttttgccgat aagcgaaccg ggtatgcttt
       61 gtgtcgcgct cggttggcac agcgccgtgt ggtcgtggtg cgaactcttc agcacatcgc
      121 ctcggaacgt aataatctgg aaacgatccg gggaactaga attcgcccgg cgttacgaaa
      181 gcgacggggc ctgctcgata aagtttacgg ttggtacgac gcggaattaa gaagaataga
      241 ttcggtgctc ttacgcctca acgcgctgtc cattggtcag caggtccaaa ctcaccataa
      301 aagctttcgg gcatcggctt gattaccgca ttagctctag gaaaatgaag gttgcgtaag
      361 ctattcgttg cgggtgaggt ggaacttggc cgtgaacgga aggtttccca cggcatgact
      421 aaagcttgcg gcgtactgaa atgggctcga cgttgagttc tcttgcctgt aagacaggcc
      481 attctcgaat ggcgaaattg ccgccgcagc ccttgcgtct ggaggactcg ttatggtcga
      541 aaaaacctta gcggatgttg gcctgatcgg tttagcggtc atgggccaga atctggccct
      601 gaatatggcc gaccatggct ttaacgttgc ggtcttcaac cggacaacgc aagtcatgaa
      661 agactttatt gccgccaacc cgaacacacc tggcggtctt gtcggatgcg aaaaactcag
      721 cgagctcatg gctgcggtca aaaagccgcg caaaattatc ctgctcgtga aagccggtcc
      781 ggcggtcgat accgtcgcaa agcagctgtt cgaagccggc cttgaagcag acgatttgat
      841 cgtggactgc ggcaacagtc agtggacgga cacgattcgc cgcgaaaagg aatattccgc
      901 tcgctgccgt ttcttcggct cgggagtttc cggcggcgag gttggcgcgc gtttcggtcc
      961 atcgctcatg cccggcggag accggcaggc ctggaaacat ctggagccga tctggagggc
     1021 gattgccgcc aaagttgatc ctaaaagcgg aaaaccgatc gaaacggcgc ggcccggcag
     1081 gccggtggaa ggcggtgttc cttgcgcggc atatattggc gagaacggcg caggacatta
     1141 cgtgaagatg gtccacaacg ggatcgaata catcgatatg cagcttatcg gggaagctta
     1201 ctttcttatt cgaaacctct tgaacgttcg gccgaatgat ctcgcagaaa tttttgccca
     1261 atgggatgag ggagacctcg attcctacct tattcagatc accgccgata ttttaaagca
     1321 gcgcgatccg cgccacccgg acgggtttct ggtcgactat gtgctcgaca cggcccagca
     1381 gaaaggcacg gggaagtgga cgagcatcaa tgccctcgat ttgggaattc ccgcaaactc
     1441 gattgcggaa gctgtttttg cccgctgttt gagcgcgtta aaagaggagc gtgtccaagc
     1501 gtcgaaacat ttgcgcggac ccgactatag ctacgacggc aagcgcgacg aattgattga
     1561 ctcggtgcgc gatgccttat actgctcgaa aatttgcgcc tatgctcagg gatttcagct
     1621 catgcgggct gccgaggttg aatacggctg gaagctcgat ttggggacca ttgcaagtat
     1681 ttggcgcggc ggctgtatta ttcgcgcgcg atttttgcag aaaatagccg acgcgtacct
     1741 gcaggatccg agactcggaa atctgttgct tgctccttat tttcaggagc agattcatca
     1801 aaaccaaaag cgctggcgga aagtcgtgac actcgccgcc gaatcgggga ctccggctcc
     1861 ggcattcatg tcggcgctgg cctattatga tggttatcga acggcgaatt tgccggcaaa
     1921 cttgctgcag gcacagcgag actatttcgg tgcgcacacg tataagcgca ccgatgaatc
     1981 gccgggtaaa tcgtttcatt tggattggcc gaaagcagat cgtcccgaga ttgagttgag
     2041 cagttgacgc gggcgtagtt ggagggttcg agatgacaag ctgaggaggg tcgtcgcata
     2101 gatgttgata tttttactgg cgacattttt ggccggctgt tctacggctt ctaattacga
     2161 tgcgatgcaa gcggacgttg atcaagcagc ggcggtggtc gaacggtttc aagccattcc
     2221 agaaaaagga attccggctg cggtgatgcg cgcggcgaga ggtctagcca tcctcacggt
     2281 cacgaaagca ggtttcatcg gtagcgtcag aga
//
LOCUS       DASULZ010000144         7395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_626586, whole genome shotgun sequence.
ACCESSION   DASULZ010000144 DASULZ010000000
VERSION     DASULZ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626586"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1487)
                     /locus_tag="VFY96_02900"
     CDS             complement(3..1487)
                     /locus_tag="VFY96_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HEX5605433.1"
                     /translation="MATAPRGLFSSANRHMQEEENLQLTSVGVDIGSSTSHLVFSRLE
                     LTLEGSRYRVTKREVLNESDILLTPYVDDTRIDVDALGSFINQQYKKAKINREQVDTG
                     ALILTGVAVRRRNARAIAELFAQEAGKFVAVSAGDGLEATMAAHGSGAVAHSGKMGGV
                     VLNIDIGGGTSKFAICNNGKVQEVSAIDIGARLLAFDQDGAVTRIEEAGRKHAAWAGF
                     SLTLGDKIPEVKLRTMVSGMVDKLFSMLKPDSVTEELANLLRLPALSYRGEIDCVMFS
                     GGVSEFIYNRAKSSFGDLGPLIAEEVHRRAGSLGLLVMEPNARIRATVIGASQYTVQV
                     SGNTIFITPENAVPVRNVAVVAPEFPLNQDEFTKEAVGAALNNALRRLDLLHGRQAVA
                     VAFHWDGSATFSRLQAFSSGIAEGLKEIFAKGHPLILVNDGDIGGIVGLHFREELQLE
                     MAIISIDGIALSDFDYIDIGALIPSSGAVPVVIKSLIFPTAPQN"
     gene            complement(1586..2782)
                     /locus_tag="VFY96_02905"
     CDS             complement(1586..2782)
                     /locus_tag="VFY96_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5605434.1"
                     /translation="MDHPAEQSFKEPSDKKTRAGYGKFLRPNTPYDNFMEAQNIPIYR
                     DIGVRRVQDLPLKPWKRTGGKGTFIQLLGTEGLWGSYVVEVPGAGALNAEKHLYEEQF
                     LVVEGRGTTEVWVDGNPKKLTFEWQKGSIFAIPMNAMHRIVNATSSPALLLAGTTAPN
                     IMNNFDNEDFIFNCPYHFKDRFDPRDDYYKYKEDVTPDPLRGLAMRQTNIIPDVMSCE
                     LPLDNRRSPGYRRIEPHMVGQQFYFWIGQHETGRYSKAHAHGSAAVLICLSGKGYTYT
                     WPAKLGTHPWEKGTTEEIRRQDYEPVGMVSAAPMSGNWFHAHFGASKEPLRLTAWFGP
                     NAPGRDRGRPGERGIDYGAIDMKDGGTAIPYNEEDPFLRKEFEETLAKVGAQSRMEPE
                     LYTHSY"
     gene            complement(2978..3481)
                     /locus_tag="VFY96_02910"
     CDS             complement(2978..3481)
                     /locus_tag="VFY96_02910"
                     /inference="COORDINATES: protein motif:HMM:NF021613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEX5605435.1"
                     /translation="MIKKLRLSATTAKILCLCYAMEPAVHTAIEVLEGDEREIIRGFW
                     SLAEDCNLFVGHNILDFDLRFIYQRSIIHEIKPSRDIPFARFRNAPVYDTMQEWSKWG
                     REHVSLEALSKALGIPSPKESLDGAKVYPYYRAGKLAEIIEYCKRDVDSARRVYRRLT
                     FTNGTLA"
     gene            complement(3591..7004)
                     /locus_tag="VFY96_02915"
     CDS             complement(3591..7004)
                     /locus_tag="VFY96_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX5605436.1"
                     /translation="MTQSASEFADAADRRAAVTTFDRSVVVTAGAGTGKTTLLIERLV
                     HLLLRNPEPLKITEIVALTFTNKAADELKLRLRQRLQTFLDSELDREPVSAFERESST
                     EIQEIIALYRLSKDELESRVRDALRNLERSDLGTIHSFAANLLRLYPLEAGLDPQFHE
                     DDGSAFEQLFDEQWSLWLDQELSLGSARAAEWQIILSRCRLDHVKALAKSLATENVDL
                     RQNSDDVTTIPAALRDWLSQLENRATILLERHPEDRLNEKLLRAAHAIISKFNQLGRK
                     LERNSEISILFSEKSLSRMTKGWSPEDIKDAQTIVRAAKGLVGVDSELIGILWRLLVT
                     YAADFRERFVREGHVSFEGLLVRARDLVRDHARVRADVKRRYRAILLDEFQDTDPIQY
                     EILLYLAEQPGQTTTDWRRVKVVPGKIFVVGDPKQSIYAFRRADIEAYLEIVEKVIQA
                     QDGIECRLTTNFRSNRTILDGVNWVFENLIQAQHGLQPHYIPLYPAPNRVSTSSPIPK
                     LLVRKIISARDMDAESARRLEGESLARWLKDEVLNKATILNNSGDQARAQSKDVAILL
                     RKLTDIHDYLLPLRREGIRYVVEGERNFYAANEIIDAVSLLRAVEDPYDRLALVGVLR
                     SPVGGLTDQQIYDLHRQNLLNYHAAEKLLGKKFPPSLAELYDALARLHEETRALSIGA
                     AVTQVFATLPVELLAACYFHGEQAVANLQKLTREAELLGREGMTTLKQAIRQLEKRVL
                     EIKDEGESALAEEHLDAVRIMSIHKAKGLEFPIVILAGCHAGVEGGRAADAEALFDWS
                     TGLTGVRIGQFSDLAGLYIAEKSRLRTAEEQKRLLYVAMTRAREHLVISCAPGSRRST
                     GSFLSMLDSTVDQRISAAQKSETLPVGKGTIEIEVVSASLTAPSTSQKPASTAADVNW
                     QGFMECWRRRALNYEGTLSSAPFVTPSRLKYQEQALTEAGDRIDGEVYDQTPALVLGD
                     LAHRFLQHWDFANDVNQFERALHDWLAHTPSTEAKSSLVQIESELQRIFAGFIRSSAY
                     SEVAASRILGREVPLVMPWNGQIMEGIIDLIYEKNGLLYLADYKTAKIVKAEVSQGVE
                     RYRPQAEIYLKAVEQSLGRAPAAFKVIFLRLGKAVELVPQDRNRELWLF"
     gene            complement(6985..>7395)
                     /locus_tag="VFY96_02920"
     CDS             complement(6985..>7395)
                     /locus_tag="VFY96_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605437.1"
                     /translation="ALRGERLQPPFYYMLAQHWAKARMQSITVSIAANFFYIAPRWLE
                     GPLISKPYAGEALTGNTGAETKRTIAYLARGVRGGRFFINRGEYCGQCDAAPICRKNH
                     PPSLWRAGKDPVTEPHRALRQKSFEIDEHDPECE"
BASE COUNT         1558 a         2034 c         2121 g         1682 t
ORIGIN      
        1 ggctaatttt gcggagctgt cggaaatatg agggacttga tcacgaccgg tacggcaccg
       61 gaagacggaa ttagcgcacc gatatcaatg taatcgaagt cgctgagcgc aatgccgtca
      121 atggaaataa ttgccatctc caactgcaat tcttctcgga agtgcagtcc aacgatgccg
      181 ccgatatcgc cgtcattgac caagatcaat ggatgacctt tggcgaaaat ctcttttagc
      241 ccttcggcaa ttccgctgga gaaagcctgt aagcgggaaa atgtcgcgga gccgtcccag
      301 tgaaaggcga ccgcaacggc ctggcgaccg tgcaggagat ccaagcgacg cagcgcatta
      361 ttgagcgcag cacctaccgc ttctttcgtg aactcatcct ggttcagcgg aaattccggg
      421 gcaacaacgg caacattgcg cacgggcaca gcgttttccg gggtaatgaa gatggtgttg
      481 ccgctcactt gcaccgtgta ttgtgacgcg ccgatgacgg tggcgcgaat acgggcattg
      541 ggctccatca cgagcagacc caagctgccg gcgcgccggt gcacttcctc ggcgatcaac
      601 ggcccgagat cgccgaagct gcttttggcc cggttataaa taaactcgga gacgccgccg
      661 gaaaacatca cgcaatcgat ctcacctcga tacgataagg ccggcaatcg caggaggttc
      721 gcgagctctt cagtcacgga gtcgggcttt aacatagaaa acaatttgtc caccattccg
      781 gaaaccatcg tgcgcaactt gacctcggga attttatctc cgagcgtcag cgaaaatccc
      841 gcccaggctg catgcttgcg gccggcttct tcgatgcgtg tgacggcgcc gtcctggtcg
      901 aacgcaagca agcgcgcgcc gatatcgatg gcggagactt cttggacttt gccgttgttg
      961 cagatggcga acttgctggt gccgccgccg atatcgatat tcaaaaccac accgcccatt
     1021 tttcccgaat gcgccaccgc gccggacccg tgcgcggcca tggttgcttc cagaccgtcg
     1081 ccggcactga cggcgacgaa ttttccagct tcctgagcga acaattcggc gatggcgcga
     1141 gcattgcggc ggcgtaccgc cacgccggtg agaatcaaag cgccggtgtc cacctgctcc
     1201 cgattgattt tggccttttt gtactgctga ttgataaaag atccgagagc atcgacatcg
     1261 atccgagtgt cgtcgacgta gggcgtcagg agaatgtccg actcgttcaa cacctcgcgc
     1321 ttagtaacgc gataacgcga accttcgagg gtcaattcga gccgggaaaa aaccaaatgc
     1381 gaagtggagg agccgatgtc gacgccgaca ctggtgagct ggagattttc ttcctcctgc
     1441 atgtggcggt tggcgctcga aaacaagcct ctcggtgcag tggccatgaa actccttcgg
     1501 gtagaagatg gttcggtgtc cagagtctag agtctggcgt tgaaccaaac cctaaactcc
     1561 agacgggaga cgctagataa acttcttagt aggagtgcgt gtagagttcc ggctccatgc
     1621 gcgactgtgc gccgaccttg gccagcgttt cttcgaattc cttgcgcaga aacggatctt
     1681 cttcgttata cggaatcgcg gtgccgccat ctttcatgtc gatggcgccg tagtcgatcc
     1741 ctctttcgcc gggtctgcct cgatcgcgcc ccggcgcatt ggggccgaac caggcggtca
     1801 gacggagcgg ctctttgctt gcgccgaaat gagcgtgaaa ccagttgccg ctcatcggtg
     1861 ccgcgctgac cataccgacc ggctcgtagt cctgacggcg aatttcttct gtcgtgcctt
     1921 tttcccaagg atgcgtgccg agctttgccg gccaagtata ggtataacct ttaccgctga
     1981 ggcaaatgag caccgccgcc gatccgtggg catgggcttt cgagtatcgc ccggtttcgt
     2041 gttggccaat ccaaaaataa aattgctggc caaccatgtg cggttcgatg cgacgatagc
     2101 ccggcgagcg acgattatcg aggggcaact cgcaggacat gacatcggga atgatattgg
     2161 tctggcgcat ggccaggccg cgcaacgggt ccggcgtgac gtcttctttg tatttatagt
     2221 aatcgtcacg cggatcgaag cgatctttga aatggtacgg gcaattgaag atgaaatctt
     2281 cgttatcgaa gttgttcatg atattgggcg ccgtggtgcc cgccaacagc agcgccgggc
     2341 tcgaggtcgc attcacgatc cgatgcatgg cgttcatcgg gatcgcaaag atggaaccct
     2401 tctgccattc gaacgtgagc tttttgggat tgccatcgac ccagacttcg gtggtgccgc
     2461 gtccctccac cactaagaac tgctcttcgt agagatgttt ctccgcattg agtgcccctg
     2521 cgcccggaac ttcgacgacg tagctgcccc acaatccttc ggtaccaagc agctgaataa
     2581 aggtgccctt gcctccggtg cgcttccacg gcttgagtgg aagatcctgc acccggcgca
     2641 cgccgatatc gcggtagatg ggaatgttct gcgcctccat gaagttgtca tagggcgtgt
     2701 tgggccggag aaattttccg tacccggccc gggtcttctt gtctgatggt tctttgaacg
     2761 attgctcagc aggatgatcc atgaatgcct ccttacgcgc ttattaaatc tttggaatct
     2821 gcaggcgtgg aatcgcgatg cttagctcaa gcccagcgga tttgaaatca attattatcg
     2881 attggctgaa ggggagtcaa acgaggcgtg actcggtatc aagtccggca gagatcgtcg
     2941 ccttgagaat cttttggtca aactcgcaac ggtgaggtta tgccaacgtg ccgttcgtaa
     3001 acgtcaggcg gcggtagact ctacgcgcag aatcgacgtc gcgtttgcag tattcgatga
     3061 tttcggcgag cttcccagct cgataatatg gataaacttt ggctccgtcg aggctttcct
     3121 tcggtgatgg aatgccaagc gctttcgaga gcgcctcgag gctgacatgc tcgcgacccc
     3181 atttgctcca ctcctgcatg gtgtcgtaaa ccggcgcgtt ccgaaagcgc gcgaagggaa
     3241 tatctcttga cggctttatt tcgtgaatga tcgaacgctg gtaaatgaaa cggagatcga
     3301 aatcgagaat gttatggccg acgaatagat tgcagtcttc cgcaaggctc cagaatcccc
     3361 tgatgatctc tcgctcgtcg ccttcgagga cttcgatcgc tgtgtgcact gcgggttcca
     3421 tggcgtagca caggcaaaga atttttgccg tggtcgccga gagacggagc ttcttgatca
     3481 aatcgccttc gttcagttgc tactgcggct cgagaaggcc atgttcccgt aaagcctgat
     3541 cggtgggcac ggtttcgatg tcgagaaaga tgaccttcat agaaagcgcg tcagaacagc
     3601 catagctccc gatttctatc ttggggaacg agctcgacag ctttgccgag ccgcaaaaaa
     3661 atcactttga atgcggcggg cgcacgcccg aggctttgct ccacagcttt caagtagatt
     3721 tccgcttgcg gccgataccg ttcgacgcct tggctgactt cggctttcac gattttagct
     3781 gtcttatagt cggccagata aagcaagcca tttttttcgt agatgagatc gataatgcct
     3841 tccataattt ggccgttcca gggcatgaca agtggcactt cgcggccgag aatgcgggag
     3901 gcggcgactt cggaataggc gcttgagcga atgaagccgg caaagattcg ctggagctcg
     3961 gactcgatct ggacgaggga ggatttggct tcggtcgaag gcgtatgagc gagccagtcg
     4021 tgaagcgcgc gctcgaactg attaacatcg ttggcgaaat cccagtgctg cagaaatcga
     4081 tgggcgaggt cgcccagcac gagcgccgga gtttgatcat aaacttctcc atcgattcgg
     4141 tcgcctgctt cggtcaaagc ttgttcctga tattttagcc gcgagggcgt aacgaacggg
     4201 gctgacgaca gcgtgccttc gtaattgagc gcgcggcgtc tccagcactc catgaaacct
     4261 tgccaattca catctgccgc cgtacttgcg ggtttttgac tggtgctggg cgcagtcaag
     4321 cttgcgctta cgacctcgat ctcgatggtt cccttgccca cggggagggt ctccgatttc
     4381 tgcgcggcgc tgattcgttg atcgaccgta ctatcaagca ttgaaaggaa gctgccggtg
     4441 gagcgccggc tgcccggtgc gcaggaaatg accaaatgct cgcgggcccg ggtcatcgcg
     4501 acatagagca gccgtttttg ttcttcagca gttcgcagcc gagatttttc cgcaatgtaa
     4561 agcccggcaa gatcggaaaa ttgaccgatt cgaactccgg tcagccccgt cgaccagtcg
     4621 aacaatgctt cagcatcggc cgctcggccg ccttccactc cggcatggca gccggcgaga
     4681 atgacaatgg gaaattccaa gcctttggct ttatggatac tcatgatgcg cacggcatcg
     4741 agatgctctt cggcgagcgc gctctcgccc tcatccttga tttcgagaac tcgtttttcc
     4801 aactggcgaa tggcttgttt gagagtagtc attccctccc gcccgagcag ctcggcctcc
     4861 cgcgtcagct tttgcagatt cgcgactgcc tgctcgccgt ggaaatagca ggccgcgagc
     4921 aactctacag gtaaggttgc gaacacctgc gtgacggcag ctccgataga caacgcgcgc
     4981 gtctcctcgt gaagacgagc gagcgcgtcg tagagctcgg ctaagcttgg cggaaatttt
     5041 ttgcccagaa gtttttctgc ggcatggtaa ttgagcagat tttgccgatg caagtcgtag
     5101 atttgctgat cggtcagacc gcccacaggc gatcgcagga cgccgacgag cgctaagcgg
     5161 tcgtacgggt cctccacggc acgaagcaag ctcacggcat cgatgatttc gttggcagca
     5221 tagaaatttc gttcgccttc gacgacatag cgaatgccct ctcgccgtag aggcagtagg
     5281 tagtcgtgaa tatcggtcag tttgcggagc aggatcgcca catctttgga ttgcgcacga
     5341 gcttggtcgc cggagttgtt caggatcgtg gccttattta ggacctcgtc cttgagccag
     5401 cgtgcgaggc tctcgccttc tagacggcgc gcgctttcag catccatatc gcgtgccgaa
     5461 ataatctttc gcaccaagag cttcggaatt ggacttgacg tgctcactcg attcggggcg
     5521 ggatagagcg gaatgtaatg cggctgcagg ccgtgttgtg cttggatgag attttcaaag
     5581 acccaattga cgccgtcgag gatagtccga ttgctgcgga agttggtggt gagccggcac
     5641 tcgatgccat cctgagcttg gatgactttc tccacgatct cgagataggc ttcaatatcg
     5701 gcgcggcgaa aagcgtagat cgattgcttc ggatccccga cgacgaatat ttttccggga
     5761 actactttca ctcggcgcca gtccgttgtc gtctgaccgg gctgttcggc cagataaagc
     5821 aggatctcat actgaatcgg atcggtatcc tgaaattcgt cgaggagaat cgcacggtag
     5881 cgccgtttca cgtcggcgcg cacgcgcgcg tgatcacgca ccaggtcgcg ggcgcgcacc
     5941 agcaaacctt cgaacgagac atggccctcg cgcacgaagc gctcgcggaa atccgctgca
     6001 taggtaacga gcaagcgcca gaggatcccg atcagttcgc tatcaacccc gacgaggccc
     6061 ttggcggcgc gcacgatggt ttgagcatct ttgatatctt ctggcgacca tcccttggtc
     6121 atgcgactga gcgatttctc ggagaacaaa atcgaaattt ccgagtttcg ctccaatttt
     6181 cgcccgagct gattgaattt gctgataatc gcatgcgccg cccgaagtaa cttttcgttc
     6241 agccggtctt cggggtggcg ttcaagcaaa attgtcgctc ggttctctag ctgagaaagc
     6301 caatcgcgca aagcggctgg aatggttgtc acatcatcgc tgttctgccg caaatcgaca
     6361 ttttccgtcg caagggattt ggccaaagct ttgacatggt cgaggcggca gcgggagaga
     6421 attatttgcc actccgccgc gcgcgcactt cccagcgaca gctcctgatc gagccaaaga
     6481 ctccactgct catcgaaaag ctgctcgaat gccgagccat cgtcctcatg aaattgtgga
     6541 tcgaggccgg cttcgagcgg ataaagacgg agcagattgg cggcgaagct atgaatggtc
     6601 ccgagatcgc ttctttccag atttctgagc gcatctcgga cccggctctc caactcatct
     6661 ttcgagagac gatagagcgc aataatttcc tggatctcgg ttgagctttc gcgctcgaac
     6721 gcgcttacgg gctcgcggtc gagctcactg tccaaaaaag tttgcaagcg ttgccggagt
     6781 ctcagcttca gctcatcggc ggctttattg gtaaaggtaa gcgcgacgat ctcggtgatt
     6841 tttagcggct cgggattacg cagcagtaga tgaaccaagc gctcgatcaa taacgtggtc
     6901 ttgccggtgc ccgcacccgc cgtgacgacg acgctgcggt cgaaggtggt gacggcggcg
     6961 cgccggtcgg cggcatcggc aaattcactc gcactctggg tcatgctcat cgatttcgaa
     7021 acttttttgc cgtaaagcgc gatggggttc agttactgga tctttgccgg cccgccacaa
     7081 actgggcggg tgatttttac gacagatcgg cgccgcgtcg cactggccgc agtattcgcc
     7141 gcggttgata aaaaaacgac cgccgcggac gccgcgggcg agataggcga tcgtcctttt
     7201 cgtttcggcg ccggtattcc cggtaagcgc ttcgccggcg tacggcttgg aaatcaacgg
     7261 accttccagc cagcgcggcg caatatagaa aaaattcgcc gcgatactca ccgttatgct
     7321 ttgcatgcgc gctttggccc agtgctgcgc gagcatgtaa tagaagggcg gttgcaggcg
     7381 ctcgccgcgg agcgc
//
LOCUS       DASULZ010000145         6419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_220196, whole genome shotgun sequence.
ACCESSION   DASULZ010000145 DASULZ010000000
VERSION     DASULZ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6419
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220196"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..952
                     /gene="hpnH"
                     /locus_tag="VFY96_02925"
     CDS             2..952
                     /gene="hpnH"
                     /locus_tag="VFY96_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018002755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HEX5605438.1"
                     /translation="MFRCNLACAGCGKIDYSEEILDRRLSLAECMAAIDECGAPVVSI
                     AGGEPLIHKEMPAIVRGFIERKKFVYLCTNALLLARRMDDYVPSPYLTFSVHLDGDRS
                     WHDRSVCRAGVFDEAVAAIQKALERRFRVTINCTLFHGQTAQEVAKFVDHVMGLGVEG
                     ITISPGFSYEHAPEQEVFFQRQASKQLFRDIFTLGKKRRWRLNHSSLFLDFLAGNQAY
                     RCTPWGNPTRNVFGWQRPCYLLVDEGYAPTFKALMEETQWYNYGTGNNPKCANCMAHC
                     GYEATAVNDSINHPLKALRVTLRGPKTEGPFAPELPVSYE"
     gene            complement(1072..2091)
                     /gene="hpnA"
                     /locus_tag="VFY96_02930"
     CDS             complement(1072..2091)
                     /gene="hpnA"
                     /locus_tag="VFY96_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011387819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HEX5605439.1"
                     /translation="MTTLVTGATGFVGAAVLRALVAAGHRVRALVRPRSDRRNLTGID
                     CEIVTGDLLEPASLQSALRGCEALFHVAADYRLWVPDRETMHRTNVQGTVDLFRAAGA
                     AGVSRIVYTSSVATLRLSNDGSSANEQSHAELSEMIGVYKQSKFLAEREVNRLAQETG
                     ISVVTVKPTTPFGPGDVKPTPTGRMIIEAASGRMPAYVNTGLNVVHVDDVAAGHLLAF
                     NRGAVGDTYILGGENRTLQWILQTVAELTGRSPPRIRLPHWFVSPLAHVAESATRLLG
                     GGVPMLTVDGVRMSRNFMYFSSAKAERELGYSARPALWALRDELEWFYENGYVVRRSR
                     KPKST"
     gene            2221..3330
                     /locus_tag="VFY96_02935"
     CDS             2221..3330
                     /locus_tag="VFY96_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605440.1"
                     /translation="MFPAIVSLLAACSGIPVHGIIAGQTIDSRVDSEVARYYVTNYLQ
                     GKHSDPALDARIDQVYKKIDGTIPGRAELKAVSDEFSADFAALLFADEISRVPLNRRF
                     RSIFDVAYDYARKAVPEGRAKLPAAADRYEALIVPTYLYNRPTLSGADLAAPRAALQK
                     VGLICQFVETSDDGPVESNAEIVMAAIRARAQSGRRLIIISASKSGAEVALALTKLRA
                     AETRHVAAWINTVGALQGTPLIDDNMIPELEFIVGKIDPAGVESMSVTRSRERFKSFQ
                     VPPQILVVNFFGIPVSGSVGFRARRGFFPMRKYGPNDGMVLLGDMIFPGGITLTELGS
                     DHFLMNDHLDVTAVALAITVIQWLENPAGENSPTP"
     gene            complement(3344..4597)
                     /locus_tag="VFY96_02940"
     CDS             complement(3344..4597)
                     /locus_tag="VFY96_02940"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX5605441.1"
                     /translation="MRAITFDISIPRLAAAKIFGAILSRAFVSSWAPVRCQHVPDPIL
                     WADDWVLVQPRLAGICGSDVIQVFMKAGLDNPLSAVVSAPHVMGHEVVGTVIETGSAV
                     KRVRKGQRVAVSPILSCLPRGLPSCESCQRGDYPLCHRFFDGRLAKGMQLGACSDVGG
                     GFAEAMSIPESMLFPIPEAVEFEAAVLADPFAVSLHAVLRAPPQRGETAVVYGSGSLA
                     LMTTLLLRVLFPETRVLAIARAYAISQFYRLGAHAVFTSKGRDLITEIGEFLKMPLRK
                     PLYGLPWLLSGVQRVYDAVGSSETFEIGVRIAKPRASIVVVGVSPPKRFEWTLLYFKE
                     LALLGSNSYGLETMDGKRCNGIEIYLDLLAQKRLDVSGLITHCYPLEHYADAFLAARN
                     KPQSKAIKVVFDPRLKVPHIEQQRN"
     gene            complement(4625..5416)
                     /locus_tag="VFY96_02945"
     CDS             complement(4625..5416)
                     /locus_tag="VFY96_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605442.1"
                     /translation="MPQLSYVEAPRGSPSATIAKWAWGLARLANPRGVLTNFNAERVT
                     GGADALPRERVEADPFVAGIARLLLTFFSVVILDLTIISLMAHRLRFWFPVWLDPEWA
                     SRSDPWVVYSQSYFAGIFLLPVLFWLVDRDFLAKRGAVTRAIFWSLCVFLFAFMIWWK
                     GELMLQYNKQYELLGWAVLTGLIWMISRRVEILPQRLRSLTRSQMLRGLLFGVSLFFF
                     VMSVLDPLVQLGLQRLPWSFGLSIEVGFFIPAGVALMTLSRRLRA"
     gene            5483..>6419
                     /locus_tag="VFY96_02950"
     CDS             5483..>6419
                     /locus_tag="VFY96_02950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5605443.1"
                     /translation="MLQKRWPVTEALWIFALAVSAVIWAGFALLPWGLWRNREVLEGT
                     RAADKGPLAEITAVIPARNEAKLIQQTLLCLAAQGTALKIILIDDGSNDCTVAVARQL
                     AHSNLRIIESAPLPDGWSGKVWALEQAWRQVMTRYILFLDADIKLTPGSINALEQQMQ
                     QQSAGFISLLAAPSMASGWEKLLMPAFVYFFKALYPFDRVNSPDSKIAAAAGGCILVE
                     TRVLQQIGGFESLKFAVIDDCAFAQRVKSHDFKIWLGLTHAAESIRGYKKLTEIGNMV
                     ARSAFAQLRYSVALLVLCTLILVLLYIAPALMIASP"
BASE COUNT         1428 a         1849 c         1810 g         1332 t
ORIGIN      
        1 gttgtttcgc tgtaatctgg cctgtgccgg gtgtggcaaa atcgattatt ccgaggagat
       61 tctcgaccgc cgcctgagtt tggccgaatg catggcggcg atcgatgagt gtggcgcgcc
      121 tgtagtttcg attgccggcg gtgagccgtt aatccacaaa gaaatgccgg cgatcgtgcg
      181 cggctttatc gagcgcaaaa aatttgttta tttatgcacc aatgctttgc tgctggcgcg
      241 ccgcatggat gattacgttc cctcacccta cctgaccttt tcggtgcatc tggacggcga
      301 tcgaagctgg cacgatcgtt cggtttgtcg cgccggggtc tttgacgaag ctgtagctgc
      361 aatccagaag gcgcttgaaa ggcgctttcg ggtgaccatc aactgcactt tgtttcacgg
      421 ccaaactgca caggaagtcg cgaagtttgt cgatcatgtg atggggctcg gagtagaggg
      481 tattacgatt tctcccgggt ttagttatga gcatgcgccc gaacaggagg tgtttttcca
      541 acgccaggcg agtaagcagc tgttcagaga tattttcacg ctaggcaaaa aacgacgctg
      601 gcggctgaac cattcgagct tgttcctgga tttcctcgcc ggtaatcagg cttaccgctg
      661 tacaccttgg ggtaacccca cgcgcaatgt tttcggttgg caacggccct gttatctgct
      721 cgtcgacgag ggttacgcgc cgactttcaa agcgctgatg gaagaaaccc agtggtataa
      781 ctacggcacc ggcaacaacc ccaaatgcgc caactgcatg gcacattgcg gttatgaagc
      841 gacggctgtg aatgattcca taaaccatcc gctcaaggcg ttgcgtgtga cgctccgcgg
      901 tcccaaaacc gaaggcccgt tcgcaccgga gttgccggtc agctatgagt gatgaagcct
      961 cagctttacc tcggccgtag cgcagagctg caggcaaggc tgcgatcgcg ctcatgctga
     1021 gtactcatcg ccttcgctct cgattgactc tcggtttaat acttgcgaac ttcaggtgga
     1081 cttaggcttt cgggaccgcc gcactacgta accgttttcg taaaaccact cgagctcatc
     1141 gcgcagcgcc caaagcgccg gccttgcact ataacctagc tcacgttcgg ccttggcgct
     1201 ggaaaaatac atgaagttgc gagacatacg aaccccatcg acggtaagca tcggtacgcc
     1261 tccaccgaga agccgggtgg cggattcagc aacgtgggcc agcggcgaca caaaccagtg
     1321 gggcagtcgt attcgcggcg gtgacctgcc cgtaagctca gcgactgtct gcaaaatcca
     1381 ttgcaatgtt cgattctcgc ctccaagaat gtaggtgtct ccaactgctc ctctattgaa
     1441 ggccagcagg tggccggccg ccacatcgtc gacgtgaaca acatttaagc cagtattgac
     1501 ataggcgggc atccggcccg aagcggcttc gataatcatt cttccggtgg gagtcggttt
     1561 gacatcgccg gggccgaacg gtgtcgtcgg cttgactgtt accaccgata taccggtttc
     1621 ctgcgcgagc cggttgacct cacgttcagc caaaaacttc gactgcttgt agaccccgat
     1681 catttcggaa agctctgcgt gcgattgctc gttcgcactg gagccgtcgt tactcaaccg
     1741 taacgtcgca acactcgagg tataaacgat gcgcgacacg cccgccgcgc ccgccgcccg
     1801 gaatagatcc accgtgcctt gaacattggt ccggtgcatt gtctctcgat caggcaccca
     1861 cagccggtaa tccgcggcga cgtggaaaag cgcctcacaa ccgcgcagcg cactctgcag
     1921 ggaggctggt tcaaggagat cgccggtgac gatctcacaa tcgatgccag tcaaattgcg
     1981 ccgatcgctc ctgggccgaa ccagcgctcg tacccggtgg ccggcggcca ccagggcgcg
     2041 cagcactgcc gcgccgacga agccggtcgc acccgtcaca agcgtcgtca tagagtatgt
     2101 tttcgttttt agtgcggaag gatgtaaata acaaattgcc aaggctgttg atttatactc
     2161 attttcgata aaaatatttt tgccgtgata aaaagaaact gccgcctcgt tctgctgagt
     2221 ttgtttcccg cgatagtatc gcttctcgcc gcatgttccg gtatcccggt gcatggcatc
     2281 attgcgggcc aaacgatcga cagccgcgta gactcggaag tagcgcgcta ctatgtgaca
     2341 aattaccttc agggtaagca cagcgatcct gctctggatg cgcggatcga ccaagtatac
     2401 aaaaaaattg acggcactat tcctggccgt gccgagctca aagcagttag cgatgaattt
     2461 tcggctgact ttgcggcgct tttgttcgcc gatgagattt cgcgtgtgcc cctcaaccgt
     2521 cggtttcgca gcatttttga cgtagcctac gactacgctc gtaaggccgt tcccgaaggc
     2581 cgtgcaaaac tgcccgccgc cgccgatcgc tacgaagcgc tgatcgtgcc gacctatctt
     2641 tacaatcgac cgaccttgag tggcgctgat ttggcagccc cgcgggcagc tctgcaaaaa
     2701 gtcggactga tctgccaatt tgtcgaaacc agcgacgacg gtcctgttga gtcgaacgcg
     2761 gaaatcgtca tggccgccat tcgcgcccgg gcccaaagcg gacggcggct aatcatcatc
     2821 agcgctagca aatcgggcgc ggaggttgcg cttgcgctga ccaagctccg agcggccgag
     2881 actcgtcatg tcgcggcttg gatcaacacg gtgggtgcac ttcagggcac gccattaatc
     2941 gatgacaata tgatccccga gctagaattc atcgtcggta aaatcgaccc agccggcgtt
     3001 gaaagcatgt ccgtcacccg cagccgggag cgattcaaat cgtttcaggt cccgccacag
     3061 attctggtgg taaatttctt cggtattccc gtgagcggca gcgttggttt tcgtgcgcgc
     3121 cgaggttttt ttccaatgcg gaagtatgga cccaacgatg gcatggtgct gctcggcgac
     3181 atgattttcc ctggcgggat cactttgacc gagcttggga gcgaccactt tcttatgaat
     3241 gaccacctcg atgtcacagc cgtggcattg gcgataactg tcatccagtg gctggagaat
     3301 cctgctggcg aaaattcccc tacgccgtga agcgctggtc ccgtcaattt cgctgctgct
     3361 cgatatgagg caccttcagg cgtggatcga agacgacttt aatcgcttta gactgaggtt
     3421 tgttgcgcgc cgccaggaag gcgtccgcat agtgttcgag cgggtagcaa tgagtgatca
     3481 acccgctcac atcaagtcgc ttttgcgcaa ggagatccag atagatttca ataccgttgc
     3541 aacgctttcc atccatggtc tccagcccat aggaattaga ccccagcagc gcgagttcct
     3601 tgaaataaag aagcgtccac tcgaaccgtt tgggcggcga gacgccgaca accacgatgg
     3661 aagcgcgcgg ttttgcgatg cgcacgccaa tctcgaacgt ttcgcttgag cccacggcgt
     3721 cgtagacgcg ctgcacccct gacaggagcc agggcaagcc gtataggggt ttccgcaacg
     3781 gcatcttaag aaactcaccg atttcggtga tgagatcccg acctttcgac gtgaatacgg
     3841 catgcgcgcc cagtctgtaa aattgtgaga tcgcataagc gcgagcgatc gcgagaaccc
     3901 gcgtctcggg aaacaacacc cgaaggagaa gcgtggtcat gagcgcgagc gatcccgatc
     3961 cataaaccac cgccgtctcg ccccgctgtg gcggcgcccg gagaaccgca tggagggaaa
     4021 ctgcgaaggg atcagcaagc accgcggcct cgaactcaac cgcctctgga atcggaaaga
     4081 gcatcgattc agggattgac atcgcctcgg cgaaaccacc gccaacatcg ctgcaggcgc
     4141 ccagttgcat gcctttggcg agcctgccgt cgaagaagcg atgacacagc ggataatcac
     4201 cgcgctggca ggactcacag gagggcagtc cgcgcggcag gcaggagagg attggcgaaa
     4261 ccgcaacccg ttgacccttc ctcactcgct tcacggcgga cccggtttcg atcaccgtgc
     4321 ccacaacttc atggcccata acgtgcggcg cggacaccac cgccgacagc gggttgtcga
     4381 gaccggcctt catgaagacc tgaataacgt cgctgccgca gatgcccgcg aggcgcggct
     4441 gcacgagcac ccaatcgtca gcccacaaaa tggggtccgg cacatgctga cagcgcaccg
     4501 gtgcccagct tgatacgaag gcgcgagaca aaatcgcgcc gaagattttg gcagcagcca
     4561 ggcgcgggat cgaaatatcg aaggtgatcg ctctcatggc tcgaatttga tctcggagct
     4621 ccgctcacgc gcgcagtcgt cgcgataacg tcatcagggc cacgcctgcc ggaatgaaga
     4681 atcccacctc gatggagagt ccgaaggacc acggcaggcg ctggaggccg agctgaacca
     4741 agggatccag gacgctcatg acaaaaaaga agagcgacac tccgaacagc aacccgcgta
     4801 gcatttggct gcgcgtcaga ctgcgaagcc gctgcggtag aatttcgacg cgccgactga
     4861 tcatccatat tagtcctgta agaacggccc aacccaacag ctcgtactgc ttgttgtatt
     4921 ggagcatgag ctctcccttc caccagatca tgaaagcaaa caggaagacg cacagcgacc
     4981 agaaaatcgc tcgcgtcacg gctccgcgtt tagcgaggaa atcacggtcc accagccaga
     5041 agaggacagg gagcagaaaa atacccgcga aatacgactg ggaatagaca acccacggat
     5101 cggaacggct cgcccactct gggtcgagcc aaaccgggaa ccagaagcgc agccgatgcg
     5161 ccattaagga aatgatcgtg agatcgagga tcacaacgga aaagaacgtc agcaggagcc
     5221 gggcaatacc ggcgacgaac ggatcggcct cgacgcgttc cctcggcagt gcatcggcac
     5281 cccccgtaac tcgctcggca ttaaaattcg taaggacccc tctcggattg gcaagacgcg
     5341 ccaaccccca tgcccatttc gcgatcgtcg ccgacggtga cccgcgcggt gcttccacgt
     5401 aacttagttg gggcaaaccc ggggttcaat gggtcacagc agaaagtttt gattgcatag
     5461 aaccgaggac cgtgattgcc tggtgctcca aaagcggtgg ccggttacgg aagctctctg
     5521 gatatttgcc ctggcagtct cggcggtgat atgggccggt ttcgctctac tgccctgggg
     5581 tctttggcgc aatcgcgagg tactcgaagg gactcgggcc gcggataaag gaccgctggc
     5641 ggaaatcacc gctgtgattc cggcgcgcaa tgaggcgaag cttattcagc agacgctcct
     5701 ttgcctagcc gcccagggaa ccgctcttaa gataatcctg atcgacgatg gatccaatga
     5761 ctgcaccgta gcagtggcgc gccagcttgc ccattcaaat cttcgaatca tcgaaagcgc
     5821 gcccctgccc gacgggtgga gcggcaaggt ttgggcgctg gaacaagcct ggcggcaggt
     5881 gatgactcgg tacattcttt ttctcgacgc cgatatcaaa ttgacgcccg gcagcatcaa
     5941 cgccctcgaa cagcagatgc agcagcaaag cgcaggcttc atttcgcttc tggccgctcc
     6001 ttccatggcg tcgggctggg aaaagctgct gatgcccgcg tttgtttatt tcttcaaggc
     6061 gctctatccg tttgatcgcg tgaactcgcc ggatagtaag atagcggcag cggcaggcgg
     6121 atgtatcctt gtggaaacgc gagttttaca gcaaatcggc ggcttcgaat cactaaaatt
     6181 tgcggtaatc gatgattgtg ccttcgctca gcgggttaaa tcacacgact ttaaaatttg
     6241 gctggggttg acgcatgcgg cagagagcat tcgaggctat aaaaaattaa ccgagattgg
     6301 caacatggtt gcccgatcgg cgttcgcgca gctccgttac tctgtcgccc ttttggtgct
     6361 ctgcacgttg atcctcgtgc tcttgtacat cgccccggct cttatgatcg cgtcgccca
//
LOCUS       DASULZ010000146         3002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_119480, whole genome shotgun sequence.
ACCESSION   DASULZ010000146 DASULZ010000000
VERSION     DASULZ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119480"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VFY96_02955"
     CDS             <1..434
                     /locus_tag="VFY96_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005286045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5605444.1"
                     /translation="GHTPAHMSWQIEDVLFVGDTLFMPDYGTARCDFPGGSAEQLFDS
                     IQRIYALPESTRLFMCHDYQPDGRELRFVTTVSEQKQSNIQLSDRTTKKEYLAFRKNR
                     DAQLAMPSLILPAVQVNIRAGEFPEPEANGTMYLKIPLNIF"
     gene            553..1038
                     /gene="tsaA"
                     /locus_tag="VFY96_02960"
     CDS             553..1038
                     /gene="tsaA"
                     /locus_tag="VFY96_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEX5605445.1"
                     /translation="MAEIVFKPIGVVRAQASESDIRRDSKEIEAELEIFPEFEPALDG
                     VDGYSHLFVLGYFNKLRPDQIGPLQVKPVRLVNRGFKLEELPVLGVFALDSPTRPNPI
                     GLTLVRFISREGRRLKVRGIDFFDGTPILDIKAYRPEYRVDEFDVPEWYKKLKDAHGH
                     I"
     gene            1442..1654
                     /locus_tag="VFY96_02965"
     CDS             1442..1654
                     /locus_tag="VFY96_02965"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX5605446.1"
                     /translation="MVPYIGKLAVEQKRWLGDQTFRDGVALCQTIPGATAMQTAAYVG
                     FRTRGVSGAAASFIGFGLPAFLLLAA"
     gene            complement(1651..2718)
                     /gene="arsB"
                     /locus_tag="VFY96_02970"
     CDS             complement(1651..2718)
                     /gene="arsB"
                     /locus_tag="VFY96_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HEX5605447.1"
                     /translation="MTTPARAKRLNFFERYLTAWVALCMLIGVVLGKTMPGMIALVRD
                     MEFGEASHINIPIAVLIWLMIYPMMLKVDFASVLQVGKRPKGLLITLFVNWLVKPFTM
                     ALFGWIFFKHLFLPLIGPELAEQYTAGVIILAAAPCTAMVFVWSYLTDGDPAYTLVQV
                     SVNDLLMLVAFAPIVTLLVSGASALAVPFLVLVYAAILFIVIPLSAGSLTRVLLVRAR
                     GLEWFEKKFLPLLHPISILALLLTLVIIFAFQADNITTRFFHVILIAVPILIQVYFNS
                     SLAYGLMRWFKVSHSIAAPGALIGASNFFELAVATAIALFGADSGAALATVVGVLVEV
                     PVMLSVCSVCNRTRPWFPQQI"
     gene            complement(2793..>3002)
                     /locus_tag="VFY96_02975"
     CDS             complement(2793..>3002)
                     /locus_tag="VFY96_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008383288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX5605448.1"
                     /translation="PVWPGQPMTAHWGITDPAAVEGDEEMRRKAFLRAYTELYRRISL
                     FTALPFETLDKLALKAKLDEIGRLQ"
BASE COUNT          753 a          837 c          825 g          587 t
ORIGIN      
        1 ccggccacac cccggcgcac atgtcctggc aaatcgagga cgtgctcttt gtcggcgata
       61 cgctcttcat gccggactac gggacggcac gttgcgactt tcccgggggg tccgctgaac
      121 aattattcga ttccatccaa cgaatctatg ctctcccgga aagcaccagg cttttcatgt
      181 gtcacgacta tcaacctgat ggccgcgagt tgcgttttgt aacgaccgtc tccgagcaaa
      241 agcaatcgaa tattcagctg agcgaccgca ccacgaaaaa ggagtacctg gcattcagaa
      301 aaaaccgcga cgctcagcta gccatgccga gtttgatttt gcctgccgtg caagtcaaca
      361 tccgcgcagg agaatttccc gagcctgagg ccaatgggac tatgtatctg aaaatcccgc
      421 tgaatatttt ttgatgcgtc gggtcagtcg cagcacaggc tcgccgcagt aacatacacg
      481 cgatcggcaa cgatgccgag aggccaaagg aagtgcttgt cgtcgaccaa atcggaagaa
      541 tgaggtgaca acatggcaga aatcgtattc aaaccaattg gcgtggtgcg agcccaagct
      601 tcggaaagcg acattcggcg cgacagcaag gagatcgaag cggagctgga aatctttccc
      661 gaattcgaac ccgctttgga cggagtagac ggctattcac atctctttgt tctgggctat
      721 ttcaacaagc tgaggccgga tcaaatagga ccgcttcaag tgaaaccggt acgactcgtg
      781 aatcgaggat tcaagctgga agagcttccg gtactgggtg tattcgcgct tgattctccc
      841 acccgcccga atccaatcgg actcacgctc gttcgtttca taagccggga aggccggcgt
      901 ctcaaagtca ggggaatcga cttttttgac gggacgccga ttctcgacat caaggcttat
      961 cggccggagt acagagtgga cgagtttgac gtgccggaat ggtacaaaaa gttaaaagac
     1021 gcgcacggac atatttaaaa cctgcaaggt aagtgcgaac gaacgcggtc agagaagccc
     1081 cggagttgag cgggaatgcg tttcacggac cgagtgtcta aaaagcgagg agctcaccgt
     1141 ccgtttggct ccggaagcga gcggctccca acgcgtagcc gttgtcgcca ccgagccttt
     1201 gactaacgat gaggagtggc agccccctgc cggaatcgaa agtggtgctg tttattgccg
     1261 ggcaaagaat ttggtaaaga cagatacgaa aagccagtgc gggcggcaag ccaagtgcgc
     1321 gcgtcgatgc caaggagaac gggtcagtgc atcaattccg ctcaagcctg tgaacccctc
     1381 tttggcccgc tcttcctgtc tttcttaaag ctcggacgca acggcattcg gcgggcccgc
     1441 catggtgccg tacatcggca agttggccgt cgaacaaaag cgctggctcg gcgatcagac
     1501 ctttcgcgat ggcgtggctc tttgccaaac gatccctgga gcaacggcga tgcaaaccgc
     1561 tgcttacgtc ggattcagaa cgagaggagt cagcggcgct gccgccagtt ttattggctt
     1621 cgggcttccc gcctttcttc tcctggccgc ttaaatttgc tgcgggaacc agggacgggt
     1681 gcgattgcag accgagcaga cggacagcat aactggcacc tctacaagca cgccgacgac
     1741 cgttgccagg gccgccccag aatcagcacc gaagagagcg atcgccgtcg ccaccgccaa
     1801 ctcaaagaag ttcgacgcgc cgatgagcgc gcctggcgca gcgatgctat gcgacacttt
     1861 gaaccacctc atcagcccgt atgccagcga tgagttgaag tagacctgaa tcaggatcgg
     1921 aaccgcgatc aaaataacat gaaagaaacg cgtcgtgatg ttatcggcct ggaacgcgaa
     1981 gatgatgact aacgtaagca acaaggccag aatcgagatg ggatgcagaa gaggcaagaa
     2041 ctttttctca aaccattcca atccccgcgc ccgcaccagc aaaacccggg tcagacttcc
     2101 tgcggacaag ggaattacga tgaaaaggat cgctgcgtat accaggacga ggaacggcac
     2161 cgccaatgcc gatgctccgc tcaccagcag cgtaacaatg ggagcgaatg caaccagcat
     2221 gaggagatcg ttcaccgaca cttgcaccaa agtataagcc gggtcaccgt cggtcagata
     2281 gctccagacg aataccattg ccgtacacgg cgcggcggcc aagatgatga ctccggcagt
     2341 atactgttcc gccagctccg gaccgatgag cggcagaaat agatgcttga aaaaaatcca
     2401 gccaaacagt gccatggtga agggcttgac cagccaattg acgaacaagg taatcaacaa
     2461 accttttgga cgtttgccaa cctgcagcac ggacgcaaaa tccaccttta acatcattgg
     2521 ataaatcatc agccagatga gcacagcgat cggaatgttg atgtgactgg cctctccgaa
     2581 ctccatgtcg cgcaccaagg caatcatacc gggcatcgtt ttacccaaga cgacgccaat
     2641 gagcatgcaa agagcgaccc acgcggtcag gtagcgctca aaaaaattga ggcgtttggc
     2701 acgcgcggga gtagtcatag tctcgacgac tttgtgcccc tggacacgcg acggctgttc
     2761 gctttgtatc tcagcggccg gttggccaag gttcattgca ggcgtccgat ctcgtcgagt
     2821 tttgccttca gcgcaagctt gtctaacgtc tcgaacggaa gcgcggtaaa cagcgatatg
     2881 cgccgataaa gctcggtata tgcccttaga aacgcctttc gtctcatctc ttcgtctccc
     2941 tcaaccgccg ctggatcggt gatgccccag tgcgccgtca tcggctgtcc gggccagacc
     3001 gg
//
LOCUS       DASULZ010000147         2543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_626781, whole genome shotgun sequence.
ACCESSION   DASULZ010000147 DASULZ010000000
VERSION     DASULZ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626781"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1641
                     /locus_tag="VFY96_02980"
     CDS             <1..1641
                     /locus_tag="VFY96_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX5605449.1"
                     /translation="NLSHGAKRMAQAKVIVKRLASIEDFGSMNVICSDKTGTLTEGIV
                     HLQSAVDVDGAPSDKVFLHAYLNAFYETGFTNPIDEAIRASRKADLTGYQKTDEIPYD
                     FLRKRLSILVAHANTHLMVTKGALANVLAVCASAEVGEGKIVDLATVQDHIQQHLEEF
                     SNKGCRTLGVAYKNMGSASRMSKVNEAGMTFLGFLVLFDPPKAKIIETIGSLKTLGVA
                     LKIITGDNHLVTANVSQQMGLSNTKILSGADLRDLSDGALLRRVADVDVFAEIEPNQK
                     ERIILALKKAGNVVGYMGDGINDASALHAADVGISVESAVDVAKEAADIVLLEKDLGV
                     LVQGVREGRTTFANTLKYVFMATSANFGNMFSMAGVSLFLPFLPLLPKQILLTNLLTD
                     FPEMTIATDKVDSEMVVYPRRWDIKAIRKFMIIFGLVSSVFDYLTFGALLTILHATQA
                     QFRTGWFLESVISASLIVLVIRSRKPFFKSRPSKYLFAATLLVVIVTVTLPFTPLGEI
                     FGFSRLPMAFLLFIGIIVMGYIVTAEIAKTIFYKKVKL"
     gene            1638..>2543
                     /locus_tag="VFY96_02985"
     CDS             1638..>2543
                     /locus_tag="VFY96_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HEX5605450.1"
                     /translation="MTDRRDRQQRSILRSIAHRAMLARGLLPEFSPEARAQLDGIHEP
                     ATRAQESTRDLRNLIWCSIDNDDSRDLDQLTVAEAMPDGATKILVAVADVDAVVKKQS
                     ALDDHARHNTTSVYTAGEIFPMLPEKLSTDLTSLNFASERLAIVIEMAVASDGSLQSS
                     DIYRATVRNHAKLAYNSLSGWLEGNGPMPSGIGKIPGLDENLRLQDSVAQKLKSLRHV
                     QGALNLDTIEAHPVFDGDELKDLAAQKKNRAKEIIEDFMIAANGVTARYLAAKMFPSL
                     RRVVRIPKRWDRIIEIASDHGFTLPE"
BASE COUNT          600 a          686 c          694 g          563 t
ORIGIN      
        1 aaccttagcc acggcgccaa acggatggcg caagcgaagg tgatcgtcaa acggctagct
       61 tcgattgaag acttcggcag catgaacgta atctgctccg ataaaaccgg aaccttgacc
      121 gaagggatcg tacacttaca atccgccgtt gacgtcgacg gtgcgccaag cgacaaagtc
      181 tttctccacg cgtatctcaa tgcattttac gagacgggct ttaccaaccc gattgacgaa
      241 gccattcgcg cgagtcgcaa ggccgacctg accggctacc agaagaccga cgagatcccc
      301 tatgattttc tacgcaagcg tttgagcatc ctggttgctc acgccaatac ccatctgatg
      361 gtaaccaaag gcgcgctagc aaatgtgctg gcagtctgtg catcagcgga agttggcgag
      421 gggaaaattg tcgaccttgc cacggtgcag gaccacatcc aacaacattt ggaggagttc
      481 agcaataagg gttgtcgcac gctgggcgtc gcttacaaaa atatgggatc tgcatcacgg
      541 atgagtaaag tcaacgaagc cggcatgacg tttttgggat tccttgtgct gtttgatccg
      601 ccgaaagcga aaatcatcga gaccatcggc agtctaaaaa ccctcggcgt ggcactgaag
      661 atcatcaccg gagacaatca cctcgttaca gccaacgtca gtcaacagat gggattatcg
      721 aatacaaaaa ttctcagcgg ggcagatctc cgtgacttga gcgatggcgc gttgctcaga
      781 cgtgtggccg atgtggacgt cttcgctgaa atcgagccga accagaaaga gcgcataatc
      841 ctcgccctca agaaggccgg caatgtcgtc ggctatatgg gagacggcat caacgatgcc
      901 tcggcgctcc acgccgccga tgttggcatt tccgtcgaga gtgccgtcga tgttgccaaa
      961 gaggcggcgg atatcgtttt gctcgaaaag gatctgggtg tgcttgtgca aggtgtgcgc
     1021 gaggggcgga cgacgttcgc gaacacgctg aagtatgtct tcatggccac cagcgccaat
     1081 tttggcaaca tgttcagcat ggccggcgta tcgctcttcc tgccgtttct gccattgttg
     1141 cccaagcaga ttttgctcac caatttactg accgattttc ccgagatgac gatcgccacc
     1201 gacaaggtgg atagcgaaat ggttgtgtat ccacggcgct gggacatcaa ggcgattcgc
     1261 aagttcatga ttatcttcgg attggtgagt tcggtgtttg actacctcac ttttggcgcg
     1321 ctgctaacca tcctccacgc tacccaggcc caatttagaa ccggctggtt tttggagtcc
     1381 gtcatttcag cgtcgctgat cgtgctggtt attcgcagtc ggaaaccttt cttcaaaagc
     1441 agaccttcga aatatttatt cgcggccact ctcttggtgg ttatcgtgac ggtgactctg
     1501 cccttcaccc cgctgggtga gatttttgga tttagccggc tgccgatggc attccttctg
     1561 ttcataggga tcattgtaat gggctacatt gtcacagcag aaatagccaa gacgattttt
     1621 tacaagaagg tgaaactatg acggatagac gcgatcgaca acaacgctct atcttgcgaa
     1681 gcatcgctca ccgggcgatg ctggcgagag ggcttcttcc cgaattttct cccgaagcgc
     1741 gcgctcagct cgacggcatc cacgagccgg caacaagagc acaagaatcg acgcgcgatc
     1801 tgcgaaatct tatctggtgt tccatagaca acgatgattc gcgggacctg gatcagctta
     1861 ccgttgccga agccatgccc gatggggcga caaagatcct tgtcgccgtt gccgatgtgg
     1921 acgctgtcgt caagaagcaa tcggcgcttg acgatcacgc gcggcacaac acgacttcgg
     1981 tctacaccgc tggcgaaata tttccgatgc tgccggagaa actctcgacc gatcttactt
     2041 cgctgaattt tgcatcagag cggctggcga tcgtcattga aatggccgtg gccagcgatg
     2101 gatcgctgca gagttcggac atttatcgcg cgacggtgcg caatcatgcc aagctggcct
     2161 acaacagtct ttccggttgg ttagagggga acggaccgat gccgtcaggg atcggcaaga
     2221 tcccgggtct agacgagaac ctccggctcc aggacagtgt cgcccaaaag ctcaagtccc
     2281 tcaggcatgt gcagggtgcg ctgaatctgg ataccatcga agcgcatccg gtgtttgacg
     2341 gcgacgaact caaagattta gcagcccaaa aaaagaaccg cgccaaagaa attatcgagg
     2401 acttcatgat cgcggcaaac ggtgttaccg cgcgctacct cgcggccaaa atgtttccgt
     2461 cgctgcggcg cgtggtgcgc atacccaaac gctgggaccg gatcattgag atcgcctcgg
     2521 accacggctt tacgctaccc gag
//
LOCUS       DASULZ010000148         2933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1032283, whole genome shotgun sequence.
ACCESSION   DASULZ010000148 DASULZ010000000
VERSION     DASULZ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032283"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VFY96_02990"
     CDS             complement(<1..208)
                     /locus_tag="VFY96_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX5605451.1"
                     /translation="MQRAKPASEDFALDDDPLWYKNAVIYELHVRAFSDGNADGIGDF
                     KGLKEKLDYLSDLGVTALWLLPFYP"
     gene            complement(214..2208)
                     /locus_tag="VFY96_02995"
     CDS             complement(214..2208)
                     /locus_tag="VFY96_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HEX5605452.1"
                     /translation="MAPPDGRSRVIIENVTPEIDGGTFAAKRVCGERVIVEADIFADG
                     HEVISGMLLFRREAEKKWHETALEFVVNDRWRAVFRVDELGRYRYTLVAWIDRFQSWR
                     RDLQKKAEVNEHTALDILTGALIIEQTAGRAAASDKKKLVEAIELLRDKDTSLSTKLT
                     AALGDDLLELVKRYPDRGGETRYDKELAVVVDREKARFSAWYEMFPRSCAAEPGVHGT
                     FKNCERLLSYVASMGFDTLYLPPIHPIGYTHRKGKNNSITAAADDPGSPWAIGSAEGG
                     HKSIHPQLGTLQDFRSFVAKAENLGLEVALDLAYQCSPDHPYVTEHPKWFRWRPDGTV
                     QFAENPPKKYQDIYPLDFTTESWRALWEELKSIVEFWREQGIRIFRVDNPHTKPLPFW
                     QWLIDTVKQDHPEVIFLAEAFTRPKVMYRLGKLGFTQSYSYFTWRNTKAEIMDYFQEL
                     TQTEVREYFRPNLWPNTPDILPEYLQFGDRAAFMIRLVLAATLSGNYGIYGPAFELFE
                     NRPRESGSEEYLDSEKYEIKHWNRESTDSLKDFIMRVNGVRKDNAALKCDWSLAFHDI
                     DNAQMICYSKQTEDLGNIIVVVVNLDPHHVQSGWVKIPLEPLQLAATDSYQAHDLLTG
                     ARFLWQGARNYVELDPKNGPAHILRLRRRIHREQDFDYFM"
     gene            complement(2195..>2933)
                     /locus_tag="VFY96_03000"
     CDS             complement(2195..>2933)
                     /locus_tag="VFY96_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEX5605453.1"
                     /translation="GESQSFISGFDPFAKTVAYCGMFNSLAQVLLKIASPGVPDIYQG
                     TEFWDLSFVDPDNRRPVDYAVRRRCLEELRGTEAQNRAGLLHDLLSSWPDGRIKLYLM
                     HKLLDFRRAHQELFAKGAYVPLEITGESSQVVCAFARQKDQAWAVAVAPRLIGSAVYN
                     GSVPLGGEFWNSTAVSFGAEMPTQWINIITGERLESTQSGSTQALSLSRVFSHFPVAL
                     LYQEQASAASSVREEQLHAAGVQHGTT"
BASE COUNT          646 a          850 c          788 g          649 t
ORIGIN      
        1 acgggtaaaa cggcagcaac cagagcgcgg tcacgccgag atcggaaagg taatcgagtt
       61 tttccttcag tcccttgaaa tcgccgatgc cgtcggcatt gccgtccgaa aatgcccgca
      121 catgcagctc ataaatgact gcgtttttat accaaagcgg atcatcgtcc aaagcgaagt
      181 cttcgctggc tggttttgcg cgttgcatag ccctcacatg aaataatcga agtcttgttc
      241 acggtgaatg cgccgccgca gacgcaagat gtgcgcgggt ccgtttttag gatcgagctc
      301 aacatagttt cgtgcgccct gccaaagaaa gcgcgcgccg gtcagcagat cgtgcgcttg
      361 ataggaatcg gtcgccgcca actgcagcgg ctccagaggg atttttaccc agcccgactg
      421 cacatgatgc gggtcgagat tcaccaccac gacgatgata ttgccgagat cttcagtttg
      481 tttgctgtag caaatcatct gcgcattatc gatatcgtgg aaagcgaggc tccaatcgca
      541 tttcagcgcg gcgttatctt tgcgaacccc gttcacgcgc atgatgaaat cttttagact
      601 atccgtgctt tcccgattcc aatgcttgat ctcgtacttt tccgaatcga gatactcttc
      661 gctgcccgac tcccgagggc gattttcgaa cagctcgaag gccggaccgt agattccata
      721 attgccgctt agtgttgctg cgagcaccag ccggatcata aaggccgcac ggtcgccgaa
      781 ctgcaagtat tccggcaaaa tatcgggcgt gttcggccac aggttgggac gaaagtattc
      841 gcgcacctcg gtttgcgtga gctcttgaaa ataatccatg atctcggcct tggtgttgcg
      901 ccaagtgaaa tagctgtagg attgcgtgaa cccaagcttg ccgagacgat acatcacctt
      961 cgggcgggta aaagcttcgg cgagaaaaat gacctcggga tgatcttgct tcaccgtgtc
     1021 aatcagccat tgccaaaatg gcaagggttt ggtgtgggga ttgtccactc gaaagatacg
     1081 gattccctgt tcgcgccaaa attcaacgat gctttttaac tcctcccata gtgcgcgcca
     1141 cgactcggta gtgaaatcga gtggatagat gtcttgatat ttttttggcg ggttttcggc
     1201 aaactgaacc gtgccgtccg gacgccaacg aaaccatttg ggatgctcgg ttacgtacgg
     1261 atgatcgggg ctgcactgat aggcgagatc gagtgcgacc tcgaggccga ggttttcggc
     1321 cttggcgaca aagctcctga aatcttggag cgtgccgagc tgcggatgaa tcgatttatg
     1381 gccgccctcc gccgaaccga tcgcccacgg gctacccgga tcatccgcag ccgccgtgat
     1441 tgaattattt ttccccttgc gatgcgtgta accgatggga tgaatcggtg gcaaatagag
     1501 cgtgtcaaac cccatcgagg ccacgtagga aagcaaacgc tcacagttct tgaaggtccc
     1561 gtgaacgccc ggctcggccg cgcacgagcg tggaaacatc tcgtaccaag cgctgaatcg
     1621 agctttctcg cggtcgacga cgacggcgag ttccttgtca tatcgggtct ccccgcctcg
     1681 atccggataa cgtttgacca gctcgagcaa atcatcgccg agcgccgctg tgagcttggt
     1741 cgataaactc gtatccttgt cgcgcaggag ctcaatcgcc tcgaccagtt tttttttatc
     1801 gctggcagcg gctcgcccag ccgtctgttc gataatgagc gcgccggtga gaatatcgag
     1861 cgcggtgtgc tcattcacct cggccttctt ctgcaagtca cgtcgccatg actggaaccg
     1921 atcgatccac gcgacaagcg tgtaccggta tcggccgagc tcgtcgacgc gaaaaacggc
     1981 gcgccagcga tcgttcacaa cgaattcgag cgctgtttcg tgccacttct tttccgcttc
     2041 tcggcggaac agaagcatac cggaaatcac ttcatggcca tcggcaaaaa tatcggcttc
     2101 gacaatcacc cgctcgccgc agacacgctt cgcagcaaaa gtcccgccgt cgatctccgg
     2161 cgtgacgttt tcgatgatga cccggcttct tccgtcaggt ggtgccatgt tgaacaccgg
     2221 ctgcatggag ctgttcctct cggacgctcg acgccgccga agcttgctcc tgatacagca
     2281 aagccacagg aaaatgactg aagacacggg acagcgacag agcctgggtc gaccccgact
     2341 gcgtcgactc cagacgttcg cccgtgatga tgtttatcca ttgggttggc atctcagcgc
     2401 caaagctcac cgcggtggag ttccagaact caccgccgag cggcacgctc ccgttataaa
     2461 cggcactccc aatcagccgc ggcgcgactg ccaccgccca cgcctgatct ttctgccgag
     2521 caaacgcgca caccacctgg ctggattctc ccgtgatctc gagcggcaca taggcccctt
     2581 tagcaaagag ttcctggtgc gcccgccgaa aatccagcag cttgtgcatc aagtaaagct
     2641 tgatccggcc gtccggccat gacgagagca gatcgtggag caaaccagcc cggttttgtg
     2701 cttctgtccc tcgcagttct tcgagacagc gccggcgcac ggcataatcc accggccggc
     2761 gattgtccgg atcgacaaag ctcagatccc aaaactcggt gccctgataa atatccggca
     2821 ctcccggcga agcgattttg aggagcactt gcgcgagaga gttgaacatt ccgcaataag
     2881 caactgtttt ggcgaacgga tcaaatccgc tgatgaacga ttggctttca ccg
//
LOCUS       DASULZ010000149         3663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1337726, whole genome shotgun sequence.
ACCESSION   DASULZ010000149 DASULZ010000000
VERSION     DASULZ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337726"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(582..1568)
                     /locus_tag="VFY96_03005"
     CDS             complement(582..1568)
                     /locus_tag="VFY96_03005"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605454.1"
                     /translation="MASRSRCFSLLVTVVPLICLPITAYSAKLYVVYASIGGTQSIGW
                     IAKEAGLFAKHGLDVDLLFTGGGRAITSLLGGDTPIITVGGPSVIAARLAGSDVIITA
                     HVFDTILYSLMVTPEIRTLADIKGKKLGASRFGSATDFALRYVLKQNGIDPVKDTVIF
                     QIGGQAETLAALKAGSIQGGVIASPATAEAKRLGMRELVNMANLGVEYPQTTIATNGR
                     YLRTNRDTLLRFTRAYVEAAHRFVNDRELSLKVIGKYTKIQNRATLDATYDDHVPYVK
                     KIPTPSAGSIKTVLEQLAVSDPKARSARPQDFYDGSIVAELEQEGFFSQVWR"
     gene            complement(1623..2468)
                     /locus_tag="VFY96_03010"
     CDS             complement(1623..2468)
                     /locus_tag="VFY96_03010"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5605455.1"
                     /translation="MRWQWYCGLLLLMMTTPAAAQPIRRSFQTTDSVTLSFLEAGSEH
                     RNKPSIVLVPGWLMPGRIWQNQLNDFSRTYRTVAFDPRGQGDSQVASSGYTAERRATD
                     LHELLLKRSNVLLIGWSLGAIDALQYIQMFGAARLAGLVLVDSSVGEEPAPGSGGGFK
                     DNLRRDRDKTLREFMRAIFAKPRPEKEIGQLVRSAKRMPLEDSLALLDYPFQRTHWRN
                     IVRSFQKPLLYVVTPQYEAQAANLKKNRPATEVELFKDAGHALFVDQPQRFNALIKKF
                     AARLS"
     gene            complement(2478..>3663)
                     /locus_tag="VFY96_03015"
     CDS             complement(2478..>3663)
                     /locus_tag="VFY96_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605456.1"
                     /translation="ALTGTLVKFMSLKRLNILTNLSHFCSLFLLVLWLMPATTEVFAN
                     ETGEYNRRATATAQILAGITPNSPDPALKHLVESETFKEHQQWMNTNWNQVRNRIQTM
                     DAWRSHEIKISGAQKKNLVYPFSGPDLLNAYVFFPDHARYIFFSLERPGTLPDLESVT
                     PAQFSKLLQDVRSAFRDIFERNYFITSYMTKQLTTPWIRGTVPVMAIMLALMNRRIIR
                     IEPLDLFPDLTHSYDVPDAKRPPVLMRAVRVDFTSATNGSGIQQLYYVSLDAADRALE
                     FYPDFLNWVSQYKPATVLIKSASYLLHDQQFSKTRTMILDAADVLVQDDTGIPFRFIS
                     QNPWQVKLYGRYHRPIRLMRYGYQKDLETAFNSRQEQNSLPFPFGYHWRGQQSGLIVA
                     HR"
BASE COUNT          853 a         1006 c          994 g          810 t
ORIGIN      
        1 gaagcaaaag ctgctttatc acaataccgt gcgactattt ggcgaaccgt aggttagctg
       61 ctgcatgttg gccgtacacc aactcaactc gtcagacccc ccgctttgtc atttcgagcg
      121 acaagcgaga aatctttctt cgtttgcagc tgaccatgga atctctcact cccgtatgaa
      181 aaatccccgt ttgaatgttt cgttcaatcg tgtgacgaca tgctgctcac cgtgagcggc
      241 ttgggcaatc tgcaaggccg atcgccgtca agatttgaaa actttgaatg ctgtttcttc
      301 gccgaacgaa acgcaagcag acccaagcac cgactccctg agtagcgtca ttattgagta
      361 gcgaaggctc atccctcgat atgcgctgcg cgcactcggg aaatcggacc acaccaaatg
      421 ttctacaggc agtgagcagc ggtgccgtac tgaaggatgc gcgtttcgcc ctcgtcgggt
      481 aaccctgaac gtctctaagc agcaacgaag ttttccggtt gctcttcgat ctggcaaaat
      541 gtgcaattca tataggttgc tacacagctt ccatgtggca tctagcgcca cacctgcgag
      601 aagaagccct cctgttccag ctcagcaacg atgctgccat cgtaaaagtc ttgcggccgt
      661 gcgctccgcg ccttcggatc actcacagcc agttgctcca aaacggtttt gatcgatccc
      721 gcagaaggag tggggatttt tttgacgtag ggcacgtgat cgtcataggt cgcatccaag
      781 gtcgcgcggt tttggatctt cgtgtacttg ccaatgacct tcagtgacaa ttcccggtcg
      841 ttgacaaacc ggtgcgcagc ctcgacatag gctcgcgtaa agcgcagcag cgtatcgcgg
      901 ttggtgcgta gataacgtcc gtttgtagcg atcgtcgtct gtggatattc gaccccaagg
      961 ttcgccatgt tgacaagctc tctcatgccc agccgctttg cttcggccgt tgccggcgat
     1021 gcgatcacac ccccctgaat cgaaccggct ttgagtgcgg ccagcgtttc tgcctgtccg
     1081 ccgatctgaa agattaccgt atctttcacc ggatcgatcc cgttttgctt cagcacatag
     1141 cgcaaggcaa aatccgtcgc cgagccaaac cggctggcgc cgagtttttt gcctttgatg
     1201 tcagccaatg tgcgaatctc aggagtgacc atgagggaat agagaatcgt gtcgaacacg
     1261 tgggcggtaa taatcacatc gctcccagcg agccgcgcgg caattaccga cgggccaccg
     1321 acagtgataa tcggggtgtc gccgcccaaa agcgaggtga tcgcgcgccc gccaccggtg
     1381 aaaagcaaat cgacatcgag accgtgcttg gcaaatagcc ctgcttcttt ggcaatccag
     1441 ccaatggact gggtgccgcc aatcgaagca taaacgacat agagcttcgc gctgtatgcc
     1501 gtgatcggaa gacaaatgag cgggacgacc gtgacgagga gactgaaaca acgggaacga
     1561 gaagccattt gggttcctcc gctctgctca aaactcgggc gagaatacaa cttgtgcgga
     1621 actcaggaaa gccgcgccgc gaatttttta atcaacgcat tgaatctttg cggctggtcg
     1681 acaaagagcg cgtggccggc gtctttgaac agttctactt cggtcgccgg tcgatttttt
     1741 ttgagattcg ccgcctgcgc ctcatattga ggcgtcacga cgtagagcaa cggcttttgg
     1801 aagctacgaa cgatgtttcg ccagtgggtc cgttgaaaag gatagtcaag caatgccagg
     1861 ctgtcctcta acggcatgcg cttggcgctg cgcaccagtt gcccaatttc cttttcggga
     1921 cgcggcttgg caaagattgc gcgcatgaac tcacggagcg tcttgtcgcg atcacgcctt
     1981 aaattgtctt taaacccacc gcccgagccg ggagccggct cttcgcccac agagctgtct
     2041 accaaaacga gcccggccag acgggcggct ccgaacattt gaatgtattg cagcgcatcg
     2101 atggccccca gggaccagcc gatcaaaaga acatttgatc ttttaagcaa cagctcgtgc
     2161 agatcggtgg cgcgccgttc cgctgtatag ccgctggaag cgacctggga gtcaccttgg
     2221 ccgcgcggat caaaggccac cgtgcggtag gttcggctga aatcatttag ctggttctgc
     2281 cagatgcggc ccggcatgag ccagcccgga accaaaacga tgctcggctt gttccggtgc
     2341 tcggagccag cctctagaaa ggagagcgta acgctgtccg tggtttgaaa tgaacggcga
     2401 atcggttgcg cggccgccgg tgttgtcatc atcaacagca agagcccaca gtaccactgc
     2461 caccgcacgg tcgctgccta ccgatgcgcg acgatcagcc cggactgctg tccccgccag
     2521 tgataaccaa aaggaaacgg cagtgaattt tgctcttggc gtgaattgaa tgccgtctcc
     2581 aaatcttttt gatacccata cctcatgaga cgaatcggcc ggtggtagcg accatagagt
     2641 ttcacctgcc agggattctg actgatgaat cgaaacggga tgccggtatc gtcctgcacc
     2701 aaaacgtcgg ccgcatcgag aatcatcgtc cgggtcttgg aaaattgctg atcgtggagc
     2761 agataggagg ccgacttgat caacaccgta gccggcttgt actggctcac ccagttaagg
     2821 aaatcaggat aaaactctaa cgccctgtcc gctgcgtcca gcgagacgta ataaagctgc
     2881 tggataccgg atccgttcgt tgcgcttgtg aagtccactc gtaccgcgcg catcagcacc
     2941 ggcggacgct tggcgtcggg cacgtcgtag gaatgtgtca gatcgggaaa aagatcgagc
     3001 ggttcaatgc gtatgatccg gcgattcatg agagccagca tgatggccat gactggaacc
     3061 gtgccgcgaa tccagggcgt cgtcagctgc tttgtcatgt aagaagtaat aaagtagttg
     3121 cgctcgaaaa tgtcgcggaa cgcgctacgc acgtcttgca acagcttcga gaactgcgcc
     3181 ggtgtgaccg attccagatc cggcagggta ccagggcgtt cgaggctgaa aaaaatgtag
     3241 cgcgcatgat ccggaaagaa gacgtaggcg ttcagcaaat ccggaccgct gaaaggataa
     3301 accagatttt ttttctgcgc gccggaaatc ttaatttcgt ggctgcgcca cgcatccatg
     3361 gtttgaattc gattgcgcac ctgattccag ttagtgttca tccactgctg gtgttctttg
     3421 aacgtttcac tctcgaccaa gtgcttcaac gctggatccg gcgaattcgg tgtaataccc
     3481 gccaagatct gcgcggtggc cgttgcgcgc cggttatact cgccagtctc attggcgaaa
     3541 acctctgtgg tggcaggcat gagccacaac accagcagga acaaggaaca aaaatgagat
     3601 aaattcgtca aaatattcaa gcgcttaaga gacataaatt taaccagcgt accagttaaa
     3661 gcc
//
LOCUS       DASULZ010000150         3276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1235928, whole genome shotgun sequence.
ACCESSION   DASULZ010000150 DASULZ010000000
VERSION     DASULZ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235928"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(281..1042)
                     /locus_tag="VFY96_03020"
     CDS             complement(281..1042)
                     /locus_tag="VFY96_03020"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014463958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX5605457.1"
                     /translation="MKLQGKTALIAGGGGGIGRAVVLALAHEGAKTAVADIVKDNAEK
                     VGSEVKALGVDAMACPVDLTKKPDVDRMVSEVLARFGQIDILINCQGWDRLEPFVESD
                     EETWEKLLAINFKSVLYTAKAVLPQMISHGGGKIVNIASDAGRVGSSWEAVYAGAKGA
                     VIAFSKTIAREVARYKINVNVVCPGLTETPLLQAVRSQSEQTARIIDAVTKATPFRRP
                     AQPEEIAEAVLFLTSPAANFITGQTLSVSGGLTMV"
     gene            complement(1039..1875)
                     /locus_tag="VFY96_03025"
     CDS             complement(1039..1875)
                     /locus_tag="VFY96_03025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HEX5605458.1"
                     /translation="MTFVAGIDMGAKSTKALILDGEKRVCGKARVKTRPDFHAVAKEV
                     LDLALQGAGLKETDLDYIATTGFGRYNVPFRDVQITDITCVGRGAVFLFPRTRCVLDI
                     GAQSTRALRVSDTGKVREFRTNDKCAAGAGGFIERACKYLEVNIDQVGDLSIQANKPQ
                     TISSVCAVLAESEIINHVSNGETIENILRGVHNSLASRALALLKRSGMEDEVTFVGGV
                     ARQKGMVKALEETLKRKVNVSEEPELVGALGAALLALRRLEKIREDKKTAVLREEARA
                     AS"
     gene            complement(1913..2746)
                     /locus_tag="VFY96_03030"
     CDS             complement(1913..2746)
                     /locus_tag="VFY96_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HEX5605459.1"
                     /translation="MIYAAGIDVGSTQTKGIIINDKHEIVARALTDTGAYVTRAAERC
                     FVEALQKSGVKREDFAYIVGTGYGRYKVTFGDAQITEISCHARGASYLFPGTRTVIDM
                     GGQDAKGIKIGEDGDVKDFVMNDKCAAGTGRFLASAAEALGMGLGDIGDISLKAKNPV
                     RLTTVCTVFVESDIMSYLAQGKKTEDILGGVHSAIAARTISLVRRVGIEPEVTFTGGV
                     SLNIGMVRALEEKLGMKLNVSPDSHFVGALGASIFALERTDKVRGEKQQTPGIAAGPT
                     A"
     gene            complement(2743..>3276)
                     /locus_tag="VFY96_03035"
     CDS             complement(2743..>3276)
                     /locus_tag="VFY96_03035"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HEX5605460.1"
                     /translation="EKARMGTGPLPEERFRFIIEGPPPWPYLRTFRDMFSKWGAVAVA
                     STYSTVGGLWEFGFRHDANHPLESIAQHMLQWNVTNRNFLQRYQQIRRYVEEWHADAL
                     VIHSVKSCRLFSAGQGDMREYFTKELNVPTMLIESDIEDPRYFSEAQLKNRIDAFFES
                     LEHKKIVRAGAEAGGAA"
BASE COUNT          641 a          966 c          889 g          780 t
ORIGIN      
        1 gatggcccgt tccatgcggc aaataagctc gccttttttc gcctcgttcc acttggcaat
       61 gaactcaggc gtggcgacgt agtacatgaa gtcaatggct tgaatttcgt tgggcatgct
      121 ctttctccgt gattgttctc gtgccctcac ccaaccctct cccagcggga gagggtgcaa
      181 gattaactgg agagcacagg ttcagaaaga agtgtaagtg atgcatcaca tcctgcctcc
      241 gtgttttcgc cctggccctc tgggaaggga ggggtcagag ctacaccatc gtcaagcctc
      301 cgctgacact gagcgtctgg ccggtaatga aattcgccgc tggcgaagtc agaaacagca
      361 cggcttcggc aatttcttcc ggctgcgccg gacggcgaaa gggtgtggcc ttcgtcaccg
      421 catcgatgat cctcgccgtc tgttccgatt ggctgcgcac agcttgcagc aacggtgttt
      481 ctgtaagccc cgggcagacc acattgacgt tgatcttata gcgcgcgact tcgcgcgcga
      541 tggttttaga aaacgcgatg acggcgccct tggcgccggc gtacaccgct tcccagctgg
      601 agccgacccg gccggcgtcg gaggcgatgt tgacgatctt gccaccgcca tgggagatca
      661 tttgcggcaa gacggcttta gctgtgtaaa ggacggattt gaaatttatc gcgagtagtt
      721 tctcccaggt ctcttcgtca ctttccacaa aaggctctaa gcgatcccac ccttggcagt
      781 tgataagaat gtcgatctga ccgaagcgcg cgagcacctc gctcaccatg cggtcgacat
      841 ctggcttttt ggtcagatcg accgggcaag ccatcgcgtc gacgccgagg gccttaacct
      901 cgctgccgac tttctcagca ttgtctttga cgatatctgc gaccgcggtc tttgcgcctt
      961 cgtgcgccaa ggcaagcaca acggcgcgcc cgattcctcc tccgcctcct gctatcagtg
     1021 cggtttttcc ttgaagcttc atgacgccgc tcttgcctcc tctctaagca cggctgtttt
     1081 cttatcttca cgaatcttct ccaggcggcg taacgcgagc agcgcggcgc ccaaagcgcc
     1141 gacgagttct ggctcttcgc taacgttcac ctttcttttg agcgtctcct ccagtgcctt
     1201 taccattcct ttttgccgcg ccacaccccc gacgaaagtg acctcgtctt ccataccgga
     1261 gcgcttgagc aacgctaagg cccgcgaagc aagtgagttg tgcacgccgc gcagaatgtt
     1321 ctcgatagtt tctccgtttg agacgtgatt gataatctct gattcagcca acaccgcaca
     1381 gacgctactt atggtttgcg gcttattagc ttgaatcgaa agatccccta cctgatcgat
     1441 attgacctcc agatatttgc aggctctttc gatgaatccg ccggcgccgg cggcgcactt
     1501 gtcgttggtg cggaactcgc gcactttacc ggtgtcgctg acgcgaagcg ctcttgtact
     1561 ctgcgcgcca atatctagga cgcagcgggt cctggggaag agaaatactg ccccccgccc
     1621 aacgcaggtg atatcggtga tttgaacgtc gcggaaggga acgttgtagc ggccaaagcc
     1681 ggtggtggcg atgtagtcca agtcagtttc tttgagtccc gccccctgaa gggctaagtc
     1741 gagcacctcc ttggcaaccg catggaaatc cggacgggtt ttcactcttg ccttgccgca
     1801 aacgcgtttt tctccatcta ggatcagcgc tttggtcgat ttagcgccca tatcgatgcc
     1861 agcgacaaac gtcatgtgac ccccctttaa ttaggcaata gacgcgaggg aattaggcag
     1921 taggccccgc cgcgattcct ggtgtctgtt gcttctcgcc tctcaccttg tcggtgcgct
     1981 ccagggcgaa aatgcttgcc ccaagcgcgc cgacgaaatg agagtccgga ctcacattga
     2041 gcttcatgcc caatttttct tccagcgcgc gcaccatacc gatgttgagc gaaaccccgc
     2101 ccgtaaaagt cacttccggt tcaatgccga cccgccgcac cagtgaaatg gttcgggccg
     2161 cgatagcgct gtgcacgccg ccgaggatat cctcggtttt tttgccttgg gcgaggtagg
     2221 acatgatgtc agactcgacg aagaccgtgc agacggtggt gaggcgcacc ggattcttcg
     2281 ccttgagcga aatgtcgccg atatcgccca ggcccatccc aagagcctcg gcagcgctgg
     2341 caaggaatct tcctgtgccg gccgcgcatt tgtcattcat aacgaagtct ttgacgtcgc
     2401 cgtcttcgcc gattttgatg cccttggcgt cctggccgcc catgtcgatc acggttcgcg
     2461 tgccgggaaa aagatagctg gcgccgcgcg cgtggcaact gatctcggtg atctgcgcgt
     2521 cgccgaaggt cactttgtac ctgccgtaac cggtgcctac tatgtaggcg aagtcttctc
     2581 gtttgactcc agatttttgc agcgcttcga cgaagcacct ttccgcagcc ctggtgacgt
     2641 aggcgcctgt gtcggtcaag gctctggcga cgatctcgtg tttgtcgttg atgataattc
     2701 ctttagtctg cgtggagccg acatcgatgc ccgcggcgta gatcatgctg cgcctcctgc
     2761 ttccgctcca gcacggacaa ttttcttgtg ttccaacgat tcgaaaaacg cgtcgatgcg
     2821 gtttttcaac tgcgcttcgg aaaaatatcg cggatcttcg atatccgatt cgatcaacat
     2881 ggtgggaacg ttcagctctt tggtaaaata ttcgcgcatg tcgccctgtc ccgcagagaa
     2941 cagccgacag ctcttaacgg aatgaatcac cagagcgtca gcatgccatt cttccacata
     3001 gcggcggatt tgctggtagc gctgcaggaa gttgcggttg gtgacgttcc attggagcat
     3061 atgctgggca atcgattcta acggatgatt ggcatcatgc ctgaagccga attcccacaa
     3121 accgccgacc gtggaatacg tcgaagccac tgccaccgcg ccccatttgg aaaacatatc
     3181 gcgaaaggtt cgcagatagg gccagggcgg cggcccttcg atgatgaagc ggaagcgctc
     3241 ttcaggcaaa ggcccggttc ccatgcgtgc tttctc
//
LOCUS       DASULZ010000151         3989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1337905, whole genome shotgun sequence.
ACCESSION   DASULZ010000151 DASULZ010000000
VERSION     DASULZ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337905"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VFY96_03040"
     CDS             complement(<1..854)
                     /locus_tag="VFY96_03040"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5605461.1"
                     /translation="MNSTADQLAQRFAAELGDDCVTVDPAQLVSRKIEGQVPALLCMP
                     ANEEQLSTALSLCAEYEAAVIPWGGGTAMAIGNAPRHMDLVISTERLARIVDHDHANL
                     TVSAESGVVLARLQTQLASQQQFAPLEPPFPDCATIGGIIAGNLNGPRRSYYGSVRDL
                     VIGIKVVLIGGEKIKAGGKVVKNVAGYDMCKLFVGSLGTLGVISAATLRLAPLPETSE
                     TVVVTGTFADVQQFANEVGRSRLLPSALILRFEGSHCQVAVHSEGFAETVARSQRDLS
                     DIADRLALA"
     gene            complement(838..2148)
                     /locus_tag="VFY96_03045"
     CDS             complement(838..2148)
                     /locus_tag="VFY96_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX5605462.1"
                     /translation="MELIQKTSEDTVPAKLAGLDIPELHQCVHCGLCLNQCPTYRVLR
                     LEPASPRGRIHLVKAAAEGRIDLGDRFKEHIYLCLLCRACESACPSGVGYGRIAETAR
                     ERLGPPGSPFARTVLNLVFTHLLPYPRRLRFLFALLRLYQGIGMQRLIQPLLPAKLRE
                     MDAMLPAIPNKFFHPKSTVLPAIGERRARVGLLDGCVMPLLFGDVNAATIRVLRRNGC
                     DVVLPKNQICCGALNIHNGESVAAKRMARRNIDVFLDANLDAIIVNAAGCGAAMKEYG
                     YLLREDPAYREKAESFSARVKDSGEFLAGLGMIAKLRTTEMTVTYQDPCHLAHGQRIR
                     AQPRQLLRMIPGLKLIELEGADRCCGSAGIYNLTHSGMSQHLLKEKMQSVAATGAEAI
                     VAPNPGCMLQLAYGSQRYGPPVKIYHLMDLLDRASDAPADEFDC"
     gene            complement(2228..3664)
                     /locus_tag="VFY96_03050"
     CDS             complement(2228..3664)
                     /locus_tag="VFY96_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5605463.1"
                     /translation="MPAAALIRELRRIVGSSYVLTEKADVVVYEQDGSIFQVMPEIVV
                     VPDTVEEIAAVVKCAQRFDTPIVPRGSGTGLAGGAVPAEGGIILSLARLNRILKVDLQ
                     NRIAVVEPGVINAEITKLVAKDDLFYAPDPSSQAACSIGGNVANNSGGPHTLAYGVTT
                     NHVLGIEVVLDDGRVVWLGGEVPDTPGYDLCGVFVGSEGTMGIVTKVVVKLTPTRESV
                     RTLLAVFNQMDDATETVVAITAAGIIPAALEIMDRTTIEAVERGAPVGLPQNAEAVLI
                     VEIEGLKEHTARALEVARSMCERNHAREVKLAADETERQLIWKGRKGAFGAMGALAPN
                     YYVQDGVVPRSQLPEMMRRIALISKQYDLRIANVFHAGDGNLHPNILFDMRSPGELER
                     VIEAGAATLRACIELGGSITGEHGVGLEKKAYIGLLFSEADLAAMTRVRRVFDPDGRF
                     NPAKLFPTPAGCGEVRLEPAKIPAGLWI"
BASE COUNT          869 a         1140 c         1139 g          841 t
ORIGIN      
        1 gccaaggcga gtctatccgc gatgtcgcta aggtcacgct gtgagcgcgc aaccgtttcg
       61 gcaaatcctt ctgagtgtac ggcgacctgg caatggcttc cctcaaatcg cagaatgagc
      121 gccgatggca ataacctaga gcggccaacc tcgttggcga attgctgcac gtcagcgaaa
      181 gttcccgtaa cgaccaccgt ctcactcgtt tccggcagcg gtgccaagcg gagcgtggct
      241 gcgctgatga ctcccagtgt cccgagggag ccgacaaaaa gtttgcacat gtcataaccg
      301 gccacatttt tcacgacctt gccgccggct ttgattttct cgccgccaat taagacaacc
      361 ttgatgccaa tcaccaggtc gcgcacgctg ccgtagtagc tgcgccgcgg gccgttcaga
      421 ttgcccgcga tgatgccgcc gatggtcgcg caatcaggaa agggtggctc gagaggcgcg
      481 aactgctgct ggctcgcgag ctgggtctgc agacgagcca agacgacgcc gctttcggcg
      541 cttaccgtaa gattggcgtg gtcgtgatcg acaatgcgag ctaatcgctc ggtgctgatg
      601 acaagatcca tatggcgcgg cgcattaccg atggccattg ccgtgccgcc gccccagggg
      661 atcactgctg cttcgtattc ggcgcaaagg ctcagtgctg tactgagctg ctcctcattc
      721 gccggcatgc aaagcaaagc cggcacttgg ccctcaattt tgcgcgaaac gagctgcgct
      781 ggatcgacag taacgcagtc gtcgccgagt tccgcggcga agcgttgcgc caattgatca
      841 gcagtcgaat tcatctgcgg gagcgtcaga cgctcgatcg agaagatcca taaggtggta
      901 gatttttaca ggaggaccat aacgttggga accgtaagca agttgcagca tgcagcctgg
      961 attgggcgcg acgatcgctt cggcgccggt agctgccacc gactgcattt tttctttcag
     1021 cagatgctgt gacatccctg aatgggtcag attgtagatc ccggcactgc cgcaacaacg
     1081 atcggcgcct tccagttcaa tgagcttcag tccaggtatc atccgcaaga gctgtcgtgg
     1141 ctgcgcgcga atccgttggc cgtgcgcgag atgacaagga tcctgatagg taacagtcat
     1201 ttcggttgtg cgtaacttgg caatcatgcc aagtcctgca aggaattcgc cgctgtcttt
     1261 aacacgggcg ctgaaactct cagctttttc tcggtaagcc ggatcctcgc gcagcaggta
     1321 accatattct ttcatggctg cgccgcagcc ggccgcattg acgatgatcg cgtcaaggtt
     1381 cgcatcgaga aagacatcga tgttgcgccg ggccattcgc ttggcggcca cgctttcgcc
     1441 gttatggatg ttcaacgcgc cacaacagat ctgatttttt ggcagaacaa catcgcagcc
     1501 gttgcgccgc aggactctta tggttgctgc gttcacgtct ccgaagagga gcggcatcac
     1561 acagccatcg agcaatccga ctctggcgcg ccgctcaccg atagccggaa gaacggtcga
     1621 tttggggtgg aaaaacttat tcggtattgc gggcagcatc gcgtccattt cgcggagctt
     1681 agccggcagg agcggctgga taaggcgctg cattccgatg ccttgataaa gacgtaacag
     1741 agcgaataaa aaccgcagtc gtcggggata aggcagcagg tgggtgaaaa ccaggttcaa
     1801 gactgtgcgg gcaaacggcg atcccggagg cccgagtcgc tcacgcgcgg tttcggcgat
     1861 gcgtccgtaa ccgacaccag aagggcatgc gctttcacag gcgcgacaga gtagacaaag
     1921 atagatatgc tctttgaacc gatcacccaa gtcgatccgt ccttctgcgg cggccttgac
     1981 cagatgaatg cgcccacgag gggaggcagg ctcgagccgc aagacacggt aggtcggaca
     2041 ctgattcaaa caaagcccgc agtggacgca ttgatgcagt tccgggatat ccaaacccgc
     2101 cagtttggca gggacggtat cctctgaagt tttctgtatc agttccaacg cagcagcttc
     2161 tccggttcta cttttctagt tctttggtgg ctgaatggaa actggaaatc gggaactacc
     2221 aactattcta gatccataac ccggccggga tttttgccgg ttcgaggcgg acttcaccgc
     2281 agccggcagg ggtaggaaag agtttcgctg gattaaacct gccgtcggga tcgaacacac
     2341 ggcgcacgcg ggtcattgcc gccaagtcgg cttcgctgaa aaggagtccg atgtaggctt
     2401 tcttttccaa gccgacgcca tgctcacctg tgatgctacc accgagctcg atgcaggcgc
     2461 gcaaagtagc ggcccccgct tcgatgaccc tctccagttc accgggactg cgcatatcga
     2521 acagaatatt gggatgcagg ttgccgtcgc cagcgtggaa aacattcgca atgcgcaggt
     2581 catattgctt gctgatgagg gcgatacggc gcatcatctc cggcagttga ctgcgcggca
     2641 ccacgccgtc ttgtacgtaa taattgggcg ccaatgcgcc catggcgccg aatgcgcctt
     2701 tgcgaccttt ccaaatcagc tgacgctcgg tttcatccgc cgcgagtttc acttcccgtg
     2761 catggttccg ctcgcacatc gagcgcgcga cctccaatgc gcgtgcggta tgctccttca
     2821 ggccttcgat ctcaacgatc agcactgctt cagcgttctg gggcaatccc actggagccc
     2881 cgcgctcaac ggcttcgatt gttgtacgat ccatgatttc caaagccgct ggaataatgc
     2941 ccgccgcggt gatagcgacg accgtttcag tcgcatcgtc catttggttg aacacggcga
     3001 gcagtgtccg caccgactcg cgggtgggcg ttagtttaac gacgaccttg gtaacgattc
     3061 ccatggtgcc ttcggagccg acgaagacac cgcagagatc ataaccggga gtatccggaa
     3121 cttcaccacc cagccagacc actcgtccat catcgagcac gacttcgata ccaagtacgt
     3181 gattggtggt tacgccgtag gcgagggtgt gcgggccgcc ggagttgttc gcgacattgc
     3241 cgccgatgga gcaagcggct tgactgctcg ggtcgggagc atagaacaaa tcgtccttgg
     3301 cgactaattt cgtgatctcc gcattgatca cgcccggctc gacaacagcg atccgattct
     3361 gcaggtcgac ttttagaatt cggttgagcc gggccagaga aagaataatc ccgccttccg
     3421 cgggcaccgc cccgcctgct agtccggtcc cggagccgcg cggcacgatc ggagtatcaa
     3481 agcgctgcgc acacttgacc actgcggcga tctcttcgac agtgtcagga acaacgacaa
     3541 tttcgggcat cacctggaaa atcgaaccgt cctgctcgta tacgacgacg tctgcttttt
     3601 cagtcaaaac ataggacgac ccgacaattc tccggagttc gcgaatcaat gctgccgccg
     3661 gcatggtgct cagaagcgta tccgaggccg gaaggtggtg tcaatcggcc tggagcgcca
     3721 tatcatttcg gctttcagtc gaattaacag acgacctaag agaccgcgcg aattttgcag
     3781 attgacgaat ttcggcgaac tgtaatataa gcggagggct taaacgtcat cgtaattagg
     3841 aggtcgtcat gtccgaatcg aaagcaattc agaaggatgg catgaaaact gtctcgcgcc
     3901 gaaatatcct gagagcggca attgccgccg gcggcgccgc agctttcggc ttcccgaata
     3961 ttgtcagggc ccaaggcgtc accaatatg
//
LOCUS       DASULZ010000152         4392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_830442, whole genome shotgun sequence.
ACCESSION   DASULZ010000152 DASULZ010000000
VERSION     DASULZ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830442"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VFY96_03055"
     CDS             <1..633
                     /locus_tag="VFY96_03055"
                     /inference="COORDINATES: protein motif:HMM:NF020287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HEX5605464.1"
                     /translation="PFLVFRPSLAQLIPNLPRSAQVVYPKDIGPILLWADIFPGARVI
                     EAGVGAGALSMALLRAIGETGQLISYEIRADFAELAKKNVFRYLGPVSNWIVKLGDVA
                     SELTENEIDRIVLDLPEPWHVVGRAWSALKPGGYLLSYLPTVLQVKTLVDALRDDTRF
                     ACIETTETLMRYWHFKGMSARPQHRMVAHTGFITSARRLADGEKDIPRAR"
     gene            complement(723..1142)
                     /locus_tag="VFY96_03060"
     CDS             complement(723..1142)
                     /locus_tag="VFY96_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5605465.1"
                     /translation="MKVKQIYHIGLPCNDLERGVKFYTEVLGMKCTKVGYDTESGGPY
                     KEKYGMFPNIARLFMEDGMCLVLFQRPSSVERGDFDEGTSHLALEVSSDDFDKASDEL
                     KKAGVKVLIDEPVVRPTGKAIYFFDTEQNYIQLWSRP"
     gene            1274..2251
                     /locus_tag="VFY96_03065"
     CDS             1274..2251
                     /locus_tag="VFY96_03065"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605466.1"
                     /translation="MKCSPLVLCYFFFLPWMLQSSAEAQQNLDRVVITYPSRSIASID
                     LYIAQERGFFRQEGLLAEVVQVRGNIGVTALLSGDAHAVNNVGTVIRAMERSDLPVKV
                     LTQSLKKNVFWLVTKPEVKSLSELKGKIFGTTTFGGAQHLAAARLLQKAGLNPDKDIT
                     VVIGGDVPAQLQSLVSGAIQLAALSPPTVIVARDKFKLKIHGSTLDDLPNLQNGLAFT
                     EKMLREKRDLVKRILRARSRAHRYFWENERGTAEVLANYLKVELPIALESYRLARPAF
                     TTNGLATDKEVQDFLRADAEVLKLKEPVTAARIFDFSLQKDVNQELGIK"
     gene            2267..3052
                     /locus_tag="VFY96_03070"
     CDS             2267..3052
                     /locus_tag="VFY96_03070"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5605467.1"
                     /translation="MGAPLIQARDITLVFKSKDREPITALRGFNLEVGQGEFVSIVGP
                     SGCGKSTFLNLLLGLIKPDSGDMTLDGSPISGPGQERAMVFQEFGLLPWRTVRANVEL
                     GLELKGISSPERAQRATELISLVGLKDFERHYPHELSGGMKQRVGLARALATEPEVLL
                     MDEPFAALDAQTRDLMQTELLQIWERTKKTVLFVTHSIEEAAYLSDRVIIMTARPGRA
                     KETLKIQLPRPRDYEMRLTPKFNDIKSRIWDVLKEELTSRVPQ"
     gene            3049..3834
                     /locus_tag="VFY96_03075"
     CDS             3049..3834
                     /locus_tag="VFY96_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5605468.1"
                     /translation="MKKRLFAEHRRFWLGLGSVLFVLAAWEVLLTYVFPLNPFFFTKP
                     SLIAVAFKEQVIYGALWGDLAVSSKAFLWGFSFAILLGIPLGALMGWRRKAGYTLDPF
                     FTALYASPLVALAPLIIVVFGVGLFAKSVLVFALSVFPFIFNTFAGVRSTDRLLINVV
                     RSFGGSEKDLYFKVIFPSTLPYIAAGARIALGRGLVGIIVGEFYAASEGIGYAISRFG
                     DTYRLPEMFAAIFLLMIAAVALTEGMRKLEKVLAPWKTTQETR"
     gene            3831..>4392
                     /locus_tag="VFY96_03080"
     CDS             3831..>4392
                     /locus_tag="VFY96_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5605469.1"
                     /translation="MKRLANFYSEREQSILGGGFILLLIVLWELMPRLVPLSKGFSLF
                     FTTPWKVIEAGYQLFVENKIQEHFYVSSIEFLAGLGLAIIVGLPLGLITGRSRDLDAM
                     VDPFVTIFNATPRLIFLPLFILWFGIGIWSKIMIVFIGAVFPLLINTYEGVKNVDRVL
                     VNVVRSFGANEWQIMKIVVLPNSVPYL"
BASE COUNT          925 a         1230 c         1179 g         1058 t
ORIGIN      
        1 ccctttctag tttttcgtcc aagcttagcg cagcttattc ccaatttacc gcgcagcgcc
       61 caggttgttt acccgaagga tatcggcccg attttgctct gggctgatat ttttcccggt
      121 gcgcgggtga tcgaagccgg cgtcggcgcc ggcgccctga gcatggcgct gctccgcgct
      181 attggtgaaa ccggtcagtt gatctcttat gaaattcgtg ccgactttgc tgagctggcc
      241 aaaaaaaatg tctttcgcta tctggggccg gtgtcgaact ggatagttaa attgggtgat
      301 gttgcaagcg agctgaccga aaatgaaatc gacagaatcg ttttggactt accggaacct
      361 tggcatgtgg tcggacgcgc ctggagcgcc ctcaagcctg gtggttattt gctttcctat
      421 ttgccgacgg tgttgcaagt aaagacgttg gtcgatgcat tgcgcgatga caccagattt
      481 gcctgtatcg aaacgactga aacactgatg cggtactggc attttaaagg gatgagcgcc
      541 cggccgcagc atcgcatggt ggcacatact ggatttatca cctcagcccg ccgtctagca
      601 gacggtgaaa aagacattcc gcgggcccgc taaaccgccg ctcagaaaac ccgagcaatt
      661 caaaatagat tggccgcggc taatccagag ccgaggatgg catctgggaa cctgaactga
      721 tcctacggcc gggaccagag ctgaatgtaa ttctgttcgg tatcgaagaa ataaattgct
      781 ttccccgttg gccgcaccac cggctcgtcg atgagaactt tgacgcccgc ttttttgagt
      841 tcatcactgg ccttgtcaaa gtcgtccgaa ctgacctcga gcgctaaatg cgaagtccct
      901 tcgtcgaaat cgccgcgctc aacgcttgac ggacgctgaa acaacaccag gcacatacca
      961 tcttccatga acaaacgcgc aatgttgggg aacatcccgt acttttcttt gtacggtcct
     1021 ccactttccg tgtcgtaacc aaccttcgtg cacttcatgc ccagcacttc ggtgtaaaac
     1081 tttacccccc gctccaggtc attgcacggc aaccctatgt ggtagatctg tttgactttc
     1141 atagcctgtt cctccgtttg attttttata ctgtaacctg aacaccggtt tcaagcaccc
     1201 aggaattgga gcgaatggcg ccgcgacggc gtcatggctt gacatccact gcgaatccac
     1261 taaacgataa cccatgaaat gctcgccttt ggtgctatgc tacttttttt tcctgccctg
     1321 gatgctgcaa agcagcgcag aagctcagca gaatctcgac cgagtcgtca taacctatcc
     1381 tagccgcagc attgcctcga tcgatcttta catcgctcag gagcgcggct tttttcggca
     1441 agaaggtctt ttggccgaag tcgtgcaagt gcgtggcaat atcggcgtga ccgcgctttt
     1501 gagcggcgac gctcacgccg tcaataacgt tggcaccgtg attcgcgcca tggaacggag
     1561 cgatcttccc gtcaaagtac ttacccaaag cctgaagaaa aacgtttttt ggctggttac
     1621 caaaccggaa gtgaagtcgc tcagtgagct caaaggcaag atcttcggaa ctaccacctt
     1681 cggcggagcg cagcaccttg cggccgcacg cctcttgcaa aaagctgggc tcaatcccga
     1741 caaagacatt acggtcgtga tcggcggtga tgttcccgcc cagctacaaa gtttggtgag
     1801 cggcgcgatc caattagccg ctttgtcgcc gcctactgtg attgtggcac gagataagtt
     1861 taaattgaaa attcatggca gcacgctcga cgatttgccc aatttgcaaa atggcctggc
     1921 gttcaccgaa aagatgctgc gggaaaaaag agaccttgtt aagcgcatcc tgcgggcacg
     1981 ctctcgggca catcgttatt tttgggaaaa cgagcgcggc accgccgaag ttctcgcgaa
     2041 ttatctaaaa gtcgagctgc ctatcgctct ggaatcttat cgcctggcgc gtcccgcctt
     2101 taccacgaat ggtctcgcca cagacaaaga agtccaagat tttctccgcg ccgacgccga
     2161 ggtattgaag ctgaaagagc ccgtaaccgc ggcgaggatt tttgacttca gcctgcaaaa
     2221 ggacgtaaat caggagttgg gaattaaata gtgtcgttgc gagcctatgg gcgcaccatt
     2281 gatccaggca cgggatatca ctttggtctt caagtccaaa gatcgagaac caatcaccgc
     2341 gctgcggggt ttcaatctcg aagtcggcca aggtgaattt gtcagcattg tcgggccgtc
     2401 gggctgcggc aaaagcactt ttctcaactt gctgttaggc ctcatcaaac ccgattcggg
     2461 tgacatgacg cttgacggct cgccgatctc tgggcccgga caagagcgtg ccatggtctt
     2521 tcaagagttc gggttgttgc cgtggcgcac cgtgcgcgcc aatgtcgagc tgggactgga
     2581 gctcaaaggg atatcgtcgc cagagcgcgc gcagcgcgcg accgagctga tcagcttggt
     2641 tggcttgaaa gacttcgagc gccactaccc ccatgagcta tccggcggca tgaaacaacg
     2701 ggtcggcctc gctcgcgccc tcgccactga acctgaagtg ctgctgatgg acgaaccctt
     2761 cgccgccctc gacgcacaga cgcgcgatct tatgcagacc gagctattgc aaatctggga
     2821 gcgcacgaaa aagaccgtgc tgtttgtcac tcacagcatc gaggaggcgg cttatctctc
     2881 tgatcgcgtc atcatcatga cggcgcggcc cgggcgggct aaagagactc taaaaattca
     2941 actgccccgg ccgcgcgatt acgagatgcg gctcacgccg aaattcaacg acatcaaatc
     3001 gcggatctgg gacgtgctca aagaagagct gacttcgcgg gtgccgcaat gaagaaaaga
     3061 ctttttgccg agcatcgccg gttctggctt ggactcggct ccgtgctttt cgtgcttgcg
     3121 gcttgggaag tgctgctgac gtatgttttt ccgctcaatc ctttcttctt caccaagcct
     3181 tctcttatcg cggtggcttt caaggaacag gtcatttacg gcgcgctgtg gggcgatctg
     3241 gcggtcagca gcaaggcgtt tttgtggggc ttcagcttcg cgattcttct gggcatacca
     3301 ttgggcgccc tgatgggttg gcgtcgcaaa gccggctaca ccctcgaccc atttttcact
     3361 gccctctacg cgagtccgct ggtcgctctc gcaccgctca tcatcgtcgt tttcggcgtc
     3421 ggtcttttcg ccaaatcggt tctggtgttc gcgctttcgg tcttcccttt catcttcaac
     3481 acctttgccg gagtgcgctc caccgaccgt ttattgatca acgtggtcag gtccttcggc
     3541 ggcagcgaaa aggatctcta tttcaaggtc atctttccga gcaccttgcc ctacattgcc
     3601 gccggtgcga ggatcgcgct cggccgcggc ctggtgggga tcatcgtggg cgaattctac
     3661 gcggcttcgg aaggcatcgg ctacgcgatc tctcgcttcg gcgacactta ccggttgccg
     3721 gaaatgttcg cggcgatttt tctgttgatg attgcggcgg tggcgctcac cgaaggcatg
     3781 agaaaactcg aaaaagtgct ggcgccgtgg aagaccacgc aggagacgcg atgaagcggc
     3841 tggctaattt ctacagcgaa agagagcaat ccattcttgg cgggggattc atcctgctcc
     3901 tgatcgtcct atgggaattg atgccgcgcc tggtaccgct gtccaaagga ttctcccttt
     3961 tcttcaccac gccatggaag gtcatcgagg ccggctatca attattcgtg gagaacaaaa
     4021 ttcaggagca cttttacgtc agctcgatcg agttccttgc cggcctcgga ctggcgatca
     4081 tcgtcgggct gccgctcgga ctcatcaccg gccgttcgcg cgacctcgat gccatggttg
     4141 accccttcgt gacgattttt aatgccacac ccaggctgat tttcctgccg cttttcatcc
     4201 tgtggttcgg catcggtatc tggtccaaga tcatgatcgt tttcatcggc gcggtatttc
     4261 ctctgctcat caacacctac gaaggagtaa aaaatgtcga tcgcgtgctg gtgaatgtgg
     4321 tgcgatcgtt cggcgccaac gagtggcaga tcatgaagat cgtcgtgctg cccaattccg
     4381 ttccctacct cg
//
LOCUS       DASULZ010000153         2281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_830707, whole genome shotgun sequence.
ACCESSION   DASULZ010000153 DASULZ010000000
VERSION     DASULZ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830707"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..815
                     /gene="dnaQ"
                     /locus_tag="VFY96_03085"
     CDS             114..815
                     /gene="dnaQ"
                     /locus_tag="VFY96_03085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEX5605470.1"
                     /translation="MREIVLDTETTGLDPTQGHRLVELGCIELLNGIPTGATFHAYIN
                     PERDMPAEAFAVHGLSAEFLKGHRRFIDVVDDFLGFIGDAPLVIHNAGFDHAFLCAEL
                     KRVERELIARERLIDTLLLARRKHPAGPNRLDDLCVRYGIDNSRRTKHGALLDAEILA
                     EVYVELIGARQTQLGLAESSERGLSGPDDAIVVRTRPQPLAPRLSETELAAHRDFVAG
                     LGENALWSYYQPAGI"
     gene            complement(816..1322)
                     /gene="secB"
                     /locus_tag="VFY96_03090"
     CDS             complement(816..1322)
                     /gene="secB"
                     /locus_tag="VFY96_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEX5605471.1"
                     /translation="MTTTNGGPAQGGPAPGAGQEQPQLTVVAQYIKDFSFENPNAPQS
                     LNVRQEQPQIGIQINVGATPLSETDIEVVIKLDGKAETSSTLLFRFELEFAGVFRIRN
                     VPQESLNPIVMIECPRLLFPFAREVIATTVRNGGFPPLLLDPVDFVSLYRQKMAQAQP
                     TPAPAAGS"
     gene            complement(1404..1850)
                     /locus_tag="VFY96_03095"
     CDS             complement(1404..1850)
                     /locus_tag="VFY96_03095"
                     /inference="COORDINATES: protein motif:HMM:NF008528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HEX5605472.1"
                     /translation="MNVAKWVILAVLALPILELAAFAAIVATIGFGWALSLILAGSLC
                     GAAILRHAGSGHVTRIRVAMDEGSFAALQTDAKGGLMLLAGILLLIPGFITDMVAWSC
                     WSGHCVMLLSAQRLDRKTASSILLPSNGTRCLTPHCLTIAKNERRH"
     gene            2058..>2281
                     /locus_tag="VFY96_03100"
     CDS             2058..>2281
                     /locus_tag="VFY96_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX5605473.1"
                     /translation="MRDVFDIYTIIFLALAVFIFLRLRSVLGQRTGRERPPYDPYSAR
                     EPVRPTPEKVVALPNRTTETVAPKPADPVE"
BASE COUNT          482 a          674 c          669 g          456 t
ORIGIN      
        1 tcgtcgtgga tacatcgaaa ggattcgacg ctgcacgtgc gcaggtacgt gaaattctcg
       61 cgcgaatagc tacaatgcca aagcgccgtc actgattctg cagcacaagc gccatgcgtg
      121 aaatcgtcct cgataccgaa acaacaggcc tcgatccgac ccagggccat cggctcgttg
      181 aactgggctg cattgaactg ctcaacggga taccaaccgg ggcgacgttc cacgcctaca
      241 tcaatcccga gcgcgacatg cccgccgagg catttgcggt ccatggtctt tccgccgaat
      301 ttctgaaagg ccacaggcgg tttatcgacg ttgtcgacga ttttcttggc ttcattggcg
      361 acgctccgct tgtgattcac aacgcgggat ttgatcacgc ctttctctgt gctgaactca
      421 aacgtgtcga gcgcgaactg attgcgcgtg aaaggctcat cgacacgctg ctattggcac
      481 gccgtaagca tccggcgggg cctaatcgac tcgatgactt gtgcgtcagg tacggcatcg
      541 acaattcgcg ccggacgaag cacggcgcac tgctcgatgc ggagatactt gccgaggttt
      601 acgtcgaatt gattggtgca cggcagacac agctcggtct cgccgaatcc agcgagcgcg
      661 ggctgagtgg ccctgacgat gcaattgtcg tccgtacgcg ccctcagcca ttggcgccgc
      721 ggttgagcga gacagaactc gccgcgcatc gcgactttgt ggctggtctc ggcgaaaacg
      781 cgttgtggag ttactaccaa cctgcgggta tttgatcagc ttcccgcggc aggcgccggc
      841 gttggctgcg cctgggccat cttctggcga tacagactga caaagtctac aggatcgagc
      901 aggagcggcg ggaaaccgcc gttacggacc gtagtcgcaa tgacctcgcg cgcaaacggg
      961 aacagcagac gcgggcattc gatcatcacg atcgggttga gactttcttg cggcacgttt
     1021 cgaattcgga acaccccggc gaattccaac tcgaatcgaa acaacaatgt ggaggacgtc
     1081 tcggctttcc catccagttt gatcacaacc tcaatgtctg tttctgacaa aggtgttgca
     1141 cccacattga tctgaattcc gatctgcggt tgttcctgcc ggacgttgag cgattgcggc
     1201 gcgtttgggt tctcgaacga gaaatccttg atgtactggg caactacggt gagctgcggc
     1261 tgctcctggc cagctcccgg tgccggcccg ccttgtgccg gccccccatt tgtcgtcgtc
     1321 ataaacgctc cttcacatca cgccactgcg cgcgcggcgg ttggctaaca tagggtcggt
     1381 gagggaacaa ggatggatgc cggtcagtgt cggcgctcat tctttgcgat ggtcaggcag
     1441 tgaggggtca ggcacctggt gccattgctc gggagcaaga tcgacgacgc cgtcttcctg
     1501 tcgaggcgct gcgccgacaa aagcatgacg cagtgacccg accagcaaga ccaggcgacc
     1561 atgtcggtaa taaaccccgg aattagcagt aaaatccccg cgagcagcat gaggcctccc
     1621 tttgcgtctg tctgcagggc cgcaaaagaa ccctcgtcca ttgctacacg gatacgggtc
     1681 acatggccgc tgccggcatg gcgcaaaatc gcagcgccgc aaagcgatcc ggccaatatc
     1741 aagcttaacg cccacccgaa gccaattgtc gcaacaattg cagcgaacgc ggcaagttcc
     1801 agaatgggaa gcgcgaggac tgccaatatt acccattttg ccacattcat cggtgtttct
     1861 ctgagggtgc gatgttggcc taggcgttct gatcgtctaa ggtccgcctg agagacctga
     1921 attgcgccga agcaccggcg cgaggcccgg cccaacggat tgatgcggcc aacaacggat
     1981 gcaagagcgg cggtgccgat cgacggcacc cgatcgtacg caaatccggc cggcgagtgg
     2041 ccacggtagc cgaaagcatg cgagacgtgt tcgatatcta taccatcata tttctagcgc
     2101 tcgcggtatt catcttcttg cgcctgcgca gcgtgttggg tcaacggacg ggcagagagc
     2161 gaccgccgta cgatccatat tcggcgcgtg agcctgtgcg gccgacgccg gaaaaagtcg
     2221 tcgcccttcc caaccggacg acggagacgg tcgcaccaaa gcctgcggat cccgtcgaga
     2281 g
//
LOCUS       DASULZ010000154         5128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_221167, whole genome shotgun sequence.
ACCESSION   DASULZ010000154 DASULZ010000000
VERSION     DASULZ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221167"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VFY96_03105"
     CDS             <1..575
                     /locus_tag="VFY96_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5605474.1"
                     /translation="AIGEYEELSQRFGFMSQQKADLEKSITDLQQTIVKLNRICRLRF
                     RESFETINEKFELIFPRLFRGGKAKLVLTDDNNLLDTGVDIIVQPPGKKLQSITLLSG
                     GEKALTAVSLLFAIFLTKPSPFCFLDEVDAPLDDANIDRFIEMIQEMSAHSQFVLVTH
                     NKKSMQAAEVLYGITMGEPGVSKVVSVRMN"
     gene            639..1562
                     /gene="ftsY"
                     /locus_tag="VFY96_03110"
     CDS             639..1562
                     /gene="ftsY"
                     /locus_tag="VFY96_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX5605475.1"
                     /translation="MAGASFFQRLKQGLSKSRENLLQNIGTIFQNRHWDEQSLETMEE
                     SLIAADVGVKATEKLMQVLRRQNPSGNGESAEMASRLQSAMIEMLAQRSSLGTIAPFS
                     VKPWVVIFLGVNGVGKTTTIGKLAAQYQNNGKKVLLVAADTFRAAAIEQLDIWAQRVG
                     VEIVKHRPGADPSAVVFDGMQAAKKRAVDVVLIDTAGRLHTKVHLLEELKKIRRVIAR
                     EQADAPHETLLVLDATTGQNGLQQARIFKEATNINGIVLTKLDGTAKGGVIISIQEEL
                     GVPVKYVGVGEEIEDLQPFEPERFVQALFEN"
     gene            1590..1958
                     /locus_tag="VFY96_03115"
     CDS             1590..1958
                     /locus_tag="VFY96_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX5605476.1"
                     /translation="MTCGYVGITLILMNLDLKLTDSAVEQVKKLLARDQRAGYGLRVG
                     VTDGGCSGFSYKLDFDNQQKPEDMMFEFGGIPIYIDRASAPYLSGTVIDFVTGLHGGG
                     FKFSNPNATGTCGCGTSFSA"
     gene            2076..3110
                     /locus_tag="VFY96_03120"
     CDS             2076..3110
                     /locus_tag="VFY96_03120"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5605477.1"
                     /translation="MKAVRIHAPGAIDALRYEDCEEPQLERPTDVTVKLIAAGVNRLD
                     LELRTGANRRFSFPHILGCDGAGRIEAMGADVRHLKPGDAVCLYPFVHCGSCPFCAAK
                     EYDRCANRSILSQSENGTYAEYITVPAKSCFPIPFGFSFEEAAAFPLVYSTAWRLMMT
                     QGKLIPGEIVLIRGLGGGIATAALQVAASGGARLILSSANDEKLARAKALGFELTIND
                     RKADLATEVRRLTAKHGVDLVVDCIGGPGWEKSLACLARGGRLVTCGAIAGAKPPTDL
                     RRLFWNHLKICGATYPTRDEFRQVLKFFDYSGVKPIIDTVYSLREAAQAHRRMEQRRH
                     FGKIVLRTGN"
     gene            3126..3713
                     /gene="rsmD"
                     /locus_tag="VFY96_03125"
     CDS             3126..3713
                     /gene="rsmD"
                     /locus_tag="VFY96_03125"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX5605478.1"
                     /translation="MRVISGKAKGRRLIAPKGGAIRPTADRIKESLFNILPRDFSSMK
                     ILELFAGTGNVSIEALSRGAESALLVDASERAARVIRENLRRLELSDRAQVWVMPVRR
                     ALNAVGRQGQKFDVIFLDPPYDQGLVGRSLELIALVNPLYPSAVVVAEHSVRETVKSS
                     YGILSLNDQRRYGDTLLSFFRHAAPLNSAGQGESS"
     gene            3710..4204
                     /gene="coaD"
                     /locus_tag="VFY96_03130"
     CDS             3710..4204
                     /gene="coaD"
                     /locus_tag="VFY96_03130"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX5605479.1"
                     /translation="MTVNNVAIYPGSFDPITNGHVDLVKRTLRVFDKIVVAIATNPEK
                     DRSLFTVDERLQMINEVFTELKGRVQADSFQGLLVDYAEKKHSTVIIRGLRAISDFEY
                     EFQMAMMNHRLKPQLETLFMMTGESEFYTSSRLVKEVVSLGGNVSGLVPDNVLKKLNQ
                     KFKR"
     gene            4215..>5128
                     /locus_tag="VFY96_03135"
     CDS             4215..>5128
                     /locus_tag="VFY96_03135"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX5605480.1"
                     /translation="MTQIAIRTKLVKPSITLAIAAKAGKLRASGIDVINFSAGEPDFD
                     TPEHIKAAAVEALRKGMTKYTDVKGIEALREAISEKYRREHDLNYRKEDILVSCGAKH
                     SLYNLLQAVVNPSDEVVIPAPYWVSYSDMALLAGGVPKLIASDEASGFRITPRQLEAA
                     LTPDTRVFFLNSPCNPTGATYSRDELLALGHVLSGHDCLIIADDIYEKIVYDGFEVSN
                     VVNLCPGLRERAVIVNGVSKTYAMTGWRIGYALGPSDVIAAAAKIQSQSTSNPTSIAQ
                     AAALEAVRGQQEEVAVMVREFQKRRDAIV"
BASE COUNT         1195 a         1390 c         1407 g         1136 t
ORIGIN      
        1 cggcgattgg cgaatacgaa gagctgagcc agcgatttgg gtttatgagc cagcaaaaag
       61 cggatctaga aaaatccatc accgacctgc agcagacgat tgtcaagctg aaccgaatct
      121 gccgcttgcg tttccgagaa agcttcgaaa cgattaacga aaaattcgag ctcatttttc
      181 cccggttgtt ccgcggcggc aaagccaagc tcgtgctcac cgacgataac aatttgctcg
      241 acaccggtgt cgacatcatc gttcagccgc ccggtaagaa acttcagtcg atcaccctat
      301 tatccggggg cgaaaaagcc ctcacggcgg tgagcctctt gtttgcgatc ttcctcacca
      361 aaccgagtcc cttctgcttc ctcgatgagg tcgacgcacc ccttgacgat gcgaacatcg
      421 atcggttcat cgagatgatc caggagatga gcgctcactc gcaattcgtg ctcgtcacgc
      481 ataacaaaaa atccatgcag gcggccgaag ttctgtatgg tatcaccatg ggcgagccgg
      541 gcgtctcaaa agtggtttcg gtcagaatga attagtttct cgctccgtcg attttccagc
      601 ccattgactc cgggcacacc ttcaaagaat atcaattcat ggccggtgcc tcttttttcc
      661 aacgcctcaa gcagggtctg tccaaaagtc gggaaaattt actgcaaaat atcggcacga
      721 tctttcaaaa tcgtcattgg gacgaacagt ctctcgaaac catggaagaa agcctgatcg
      781 cggcggacgt tggcgtcaaa gcgactgaaa agctgatgca agtgttgcgt cggcaaaacc
      841 cgagcggcaa tggcgagtcg gccgaaatgg cgtctcgtct gcaatctgcg atgatcgaga
      901 tgctggcaca aagatcatcg ctcggcacaa tcgcgccgtt ttccgtcaag ccctgggtcg
      961 tcatctttct cggtgtcaat ggcgttggta agacgacaac cattggcaaa ctcgcagcgc
     1021 aataccaaaa caatgggaaa aaagtcctcc tggtggcagc cgatacgttt cgcgccgccg
     1081 ccatcgagca actcgatatt tgggctcagc gagttggcgt ggaaatcgtc aaacaccggc
     1141 ccggcgcgga cccctcggcg gtcgttttcg atggcatgca ggccgctaaa aaacgcgctg
     1201 tcgatgttgt gcttatcgac actgccggcc ggcttcacac gaaagtgcat ttacttgaag
     1261 agctcaaaaa aatccgccgc gtcatcgcgc gtgagcaagc ggatgcgccc catgaaactc
     1321 tgctcgtact cgacgccacc accggccaga atggcttaca gcaggcgcgc atttttaagg
     1381 aagccaccaa tatcaacggc attgtgctga ccaagctgga tggcaccgct aaaggcgggg
     1441 tgattatcag tatccaagag gaactcgggg ttccggtgaa atatgtcgga gtcggcgaag
     1501 agattgagga ccttcaaccc ttcgaacccg agcgcttcgt gcaagcgcta tttgaaaact
     1561 gaggtggcga ccccccccgg cgatccgagt tgacatgcgg atacgtcggc attaccctaa
     1621 tactgatgaa cttggaccta aagcttacgg atagcgcggt cgagcaggtg aaaaaacttt
     1681 tggcgcgcga tcaacgtgct ggatatgggc ttcgcgtcgg tgtcaccgat ggcggctgtt
     1741 ccggcttttc ttataagctt gacttcgaca atcagcaaaa gccggaagat atgatgttcg
     1801 agttcggcgg tataccgatc tacatcgatc gcgccagcgc cccatatctc agtgggacgg
     1861 tcatcgactt cgtcaccggt ctgcacggcg gcggcttcaa gttttccaac cccaatgcca
     1921 ccggcacttg cggctgcggc acgtcatttt ctgcatagcg cccgatctcg gctggttttg
     1981 ctttcctgaa gcgcagttcg tcagttggac tgtttccatc tttgcatcgt cgccgactcc
     2041 cgagcgtatt ttacgcgtga tcgggagaca atcgattgaa agccgtaaga atacacgccc
     2101 ctggggccat cgatgcatta cgctatgagg actgcgaaga accgcagctg gaacggccca
     2161 ccgatgttac cgttaaatta atagcggccg gagtcaatcg tctcgatctt gagcttcgaa
     2221 ccggcgctaa caggcgattc tcctttccac acattctcgg ctgtgacgga gccgggcgaa
     2281 ttgaagcgat gggcgccgac gttaggcatc ttaaaccggg cgatgcggtc tgcctttatc
     2341 cgttcgtcca ctgcggcagc tgtccgttct gcgctgctaa agagtacgac cgttgcgcca
     2401 atcgttctat tttgagccaa agtgaaaatg gtacctacgc tgaatacatt acggtcccgg
     2461 cgaagagttg ctttccaatt ccgttcggat tctcgtttga ggaggcggcg gcctttccgc
     2521 tcgtctacag caccgcctgg cgtttgatga tgactcaagg aaaactcatc cctggcgaga
     2581 tcgttctgat ccgtggcctc ggtggtggca tcgcaacggc ggcgctccag gtcgccgcct
     2641 ccggcggagc ccggctcatc ctaagctcag cgaacgatga aaaactcgcc agggcgaaag
     2701 cgcttggatt cgagttgacg atcaatgacc gaaaagcaga cttagccacg gaagtgagac
     2761 gcctgaccgc taaacacggt gtcgatctcg tggtcgattg catcggcgga cccggttggg
     2821 agaagagttt ggcctgtctt gctcgcgggg ggcggctcgt cacctgcggc gcgatcgctg
     2881 gcgcaaaacc gccgaccgat ctgcgccgac ttttctggaa ccacttaaaa atttgcggcg
     2941 ccacctaccc tacccgtgac gaatttcgac aggtgctcaa gtttttcgat tactccggag
     3001 taaaaccgat catcgacaca gtttactcgc tcagggaagc cgcacaggca caccggcgca
     3061 tggaacaacg gcgtcacttc ggaaaaattg ttctgcgcac cggcaattaa gctcaggaac
     3121 gagagatgcg cgtcatcagc ggaaaagcta aaggccggcg gctcattgct cccaaaggcg
     3181 gtgcgattag accgacagcc gaccgcatta aggaatcgct gtttaatatt ttgccgcggg
     3241 atttttccag catgaagatt cttgagctct ttgcggggac tggaaatgtc agcattgagg
     3301 ctttgagccg cggcgcggaa agcgcgctct tggtcgatgc ctcggagcga gcggctcgtg
     3361 tcattcgcga aaatctgcgg cgcctcgaac ttagtgatcg cgcacaagta tgggtcatgc
     3421 ccgtgcgccg agcgctcaac gccgtggggc ggcagggtca aaagtttgat gttatttttc
     3481 tcgatccgcc ctacgatcag gggctggtcg gacgctcgct cgagctgatt gcgttggtca
     3541 atccgcttta tccgagcgcg gtggtagtcg ccgagcatag cgtgcgcgag acggtgaaat
     3601 cgagctatgg gattcttagc ttgaatgatc aacggcgata tggtgatact ctgctgtcgt
     3661 ttttcagaca cgcagcgcca ttgaattccg ctggtcaagg agagagttca tgacggtcaa
     3721 caatgtcgcc atctatcctg gctcgttcga tccgatcacc aatggccatg tggacctcgt
     3781 gaaacgcaca ctgcgcgtat ttgacaaaat cgtcgtcgcg atcgcgacca atccggaaaa
     3841 ggatcgatct ctcttcaccg tcgatgaacg tctccagatg atcaacgaag ttttcaccga
     3901 gcttaaaggc agagtgcagg ccgactcttt tcaagggttg ctggtggatt acgcggaaaa
     3961 aaagcactcc accgtaatca ttcgcggtct gcgcgccatt tcagatttcg agtatgagtt
     4021 tcagatggcg atgatgaatc atcgtctgaa accgcagctt gaaaccttgt ttatgatgac
     4081 cggcgaatcc gagttctaca ccagctcacg gctggtgaag gaagtggtca gtcttggtgg
     4141 gaacgtttcg gggctggttc ccgataacgt gttgaaaaag ctaaatcaga aatttaaacg
     4201 ataaaggcca cttcatgacc caaatcgcca tcaggacaaa actggtcaaa ccttcgatca
     4261 cgctcgcaat tgccgcaaaa gccggaaagc tgcgcgcttc aggcattgac gtgatcaatt
     4321 tttccgcggg tgaaccggat ttcgatacgc ccgaacacat taaggccgcc gcggtcgaag
     4381 cgctccgtaa aggaatgacc aagtataccg acgttaaagg gattgaagct ttgcgcgaag
     4441 cgattagcga aaagtatcgc cgcgagcatg acttgaacta ccgcaaagaa gatatcctgg
     4501 tcagctgcgg cgccaaacat tcgctttaca acttactgca agccgtggtg aaccctagcg
     4561 atgaggtggt cattcccgct ccctactggg tgagctattc cgacatggca ttattggccg
     4621 gcggtgtgcc gaaactgatc gcgtcggatg aggcgagcgg ttttcgtatc actccccgac
     4681 aactcgaagc ggcgttgaca ccggacactc gggtcttttt tctaaacagt ccctgtaatc
     4741 ccaccggcgc gacctacagc cgtgacgagc tccttgcgct cggtcacgtt ctctccggac
     4801 acgattgtct catcattgcg gacgacatct acgaaaaaat cgtttacgac ggctttgaag
     4861 tgagcaatgt cgtaaacctg tgccccgggc ttcgtgagcg ggcggtgatc gtcaacggag
     4921 tgtcaaagac ctatgccatg acgggttggc gaatcggtta cgcgctcgga ccgagcgacg
     4981 taatcgccgc ggcagcaaaa attcaaagtc agagcacttc gaatccgacc tcgatcgccc
     5041 aagcagcggc tttggaagcc gtgcgcgggc agcaggaaga agttgcggtc atggtgcgtg
     5101 aatttcaaaa acggcgagat gcgattgt
//
LOCUS       DASULZ010000155         5076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1236396, whole genome shotgun sequence.
ACCESSION   DASULZ010000155 DASULZ010000000
VERSION     DASULZ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5076
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236396"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2208
                     /locus_tag="VFY96_03140"
     CDS             <1..2208
                     /locus_tag="VFY96_03140"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5605481.1"
                     /translation="TPTPRLEGPDKVSGKAIYASDVCLPGMLWCKVLRSPIPYGRIKG
                     IDVSRARALSGVAAVVTGQDVAGLLIGRKIYDMPILADGVVRFIGEKVAAVAAETEDI
                     AEEAVRLIEVDYEELPPILDPLEALEPSAPLLHPNVQHYRGLLHPIQQPSNVFIDMKW
                     SKGDIEAGFRTADFIIENTFTTRPVHQAYIEPHACVVQARAGGGADIWGCSKVPFALR
                     EQVAIAFGQSVDNFVVHPCYIGGCFGGKGDFMDVPACYLLSLKTGRPVKTVMDYGEEF
                     IAGNPRHAAIVRVKSGVKNGRLVAHRMDYVFDSGAYAAFKPQGYLVGPKEAAGPYNIP
                     NVFIEEKIVYTNKIPCGHMRAPGDPQGFFANESQMDLIARRLNMDPIAFRKKNLMGDG
                     DVSPIGHVVPHIKSHEIVDEAVRASGYEKPKGKQCGRGVAVAQWLPLGGECHAFVTID
                     VEGSVTVATAMLDQGAGTHTAMRLVAAEELKLPLERIRIRTLDTSEVGPDTGIGASRG
                     TRIFGNATRLAAAEAREQLLEAASRKFETPKQQIELRGEGTMQTAAGHSISYGELAAF
                     CGGEVRGHGFYRNFNSGPEAALCIQVAEVSVDVETGQIKLRQFTTAHSTGTILNPLMH
                     QAQIDGGVVMGMGYSVMEGMSIDEGKVTTTNFGENKIPSFRDIPPLKTVVQEFPVGNG
                     PYGGMSIGEPPVVPTAAAIANAVHDAVGVRIKDLPITAEKVLNALKALTQRVT"
     gene            complement(2277..2645)
                     /locus_tag="VFY96_03145"
     CDS             complement(2277..2645)
                     /locus_tag="VFY96_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605482.1"
                     /translation="MKQDLLPPSLPTGVIVAESYTRAREALADLLRCDGHRAFEANAE
                     TALFAINSNTDAGVLLLDLDMANWKTILNYAQTRIPGIFVIGMRGSEPVPPDLEESGI
                     RVCFKKPLSYRAISQVISAR"
     gene            complement(2858..3658)
                     /locus_tag="VFY96_03150"
     CDS             complement(2858..3658)
                     /locus_tag="VFY96_03150"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HEX5605483.1"
                     /translation="MNTGATASASLIAGCAVNEAPLIEQTSHRTWPLPETAWFLFMRW
                     HDLLFLHWPVRPETIRPLIPRVLELDTFDGWCWIGIVPFRMTGVRPHYLPIPLAFPEL
                     NVRTYVKTPHKTGVWFFSLDAANWLAVRAARWLGMPYYDARMKVGSAGESVDYKSLRD
                     HKKAAAAEFDATYAPTGETYMAQTGTLDHWLTERYCLFSALRPDRIVFGEIHHPQWRL
                     QPATVELRSCTMLQASGIELSNSQPLCHFSRLQEVVAWPIVPIEHAEI"
     gene            3873..4379
                     /locus_tag="VFY96_03155"
     CDS             3873..4379
                     /locus_tag="VFY96_03155"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5605484.1"
                     /translation="MKNELAQRLVRGLKEAGIDFVTYLPETRLSQIVPLIRDDPAMEL
                     IAASSEQEAVTIAVGATLGGRQGAVYMENTGVYVSSYSLLVTGKQLGVPILLVIGYLG
                     GVPDQRNSFLYATIGKHTVPVLEGLGIGYQIIEDGQKLENKIRDAVRAANAQRAPYAL
                     LFAGEFSE"
     gene            4376..4978
                     /locus_tag="VFY96_03160"
     CDS             4376..4978
                     /locus_tag="VFY96_03160"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5605485.1"
                     /translation="MIRHACLELLAGKMTDQLVVTSQSGQRIEWSHVSKHEGNLLLGM
                     MGCAIGVATGLALALPHRKVIALDSDGSVLLSLFNLATLGNLRPKNLVVYVFDNGVYS
                     GSRISYPTATAGNTDLAAMARAAGISNACTITEISEFESKGIGALTSNELGFFVCKVE
                     ESLLHREIPRPNTDLAENKYTFVRYLERTEGKTISFVGRG"
BASE COUNT         1153 a         1333 c         1500 g         1090 t
ORIGIN      
        1 acgccgaccc cgcgcctcga gggtcctgac aaggtctcgg gtaaagcaat ttacgccagc
       61 gacgtgtgct tgcccggcat gctttggtgc aaggtactga ggagtccgat cccttacgga
      121 cgcatcaaag gaatcgatgt gagccgcgcg cgcgctttat ccggcgtggc tgccgtggtt
      181 accggacaag acgtcgcggg tttgcttatc gggcgcaaga tttacgacat gccaatcctg
      241 gccgacgggg tggttcgttt tatcggcgag aaagtcgccg cggtcgcggc ggaaacggaa
      301 gatattgccg aagaggcggt gcgtctcatc gaggtagact acgaagagtt gccgccgatt
      361 ctcgaccctc tcgaagcgct cgagccctca gcgccgttgc ttcatcccaa cgtgcagcac
      421 tatcgcggtt tgctgcatcc gatccaacag ccgagtaacg tgtttatcga catgaaatgg
      481 agcaagggcg atatcgaggc cggatttcgc actgccgact tcatcatcga gaatactttc
      541 accacccgac ccgtgcatca ggcttacatc gaaccccatg cctgtgtcgt acaagcgcgg
      601 gccggcggcg gcgccgatat ctggggctgt tcgaaggtac cctttgcctt gcgcgaacag
      661 gtcgctatcg ctttcggtca aagcgtcgac aattttgttg tgcatccatg ctacatcggc
      721 ggttgcttcg gcggcaaagg tgattttatg gatgtgccgg cctgctatct gttgtcgctc
      781 aaaaccggac ggcccgtgaa gacggtcatg gattacggcg aagaattcat tgccggtaat
      841 ccgcgccatg ctgcgatcgt gcgagtaaaa agcggcgtga aaaacggtag gctggtcgcg
      901 catcggatgg attacgtctt cgatagcggc gcctatgcag cgttcaagcc gcaagggtac
      961 ctggtcggtc caaaggaggc cgcggggccg tacaacatcc ccaatgtctt cattgaggaa
     1021 aaaatcgtct atacgaacaa gattccgtgc gggcacatgc gcgcgcctgg cgatccgcag
     1081 ggatttttcg ccaacgagtc gcagatggat ctgatcgcgc gccgattgaa catggatccg
     1141 atcgcgttca gaaagaaaaa tctcatgggc gacggcgacg tctcgccgat cgggcacgtt
     1201 gtgccccata tcaagagcca tgagatcgtc gacgaggcgg tgcgcgcgtc cgggtatgaa
     1261 aaaccaaaag gcaaacaatg cggccgcgga gttgcggtgg cgcagtggct gcccttgggc
     1321 ggagagtgcc atgcctttgt gactatcgat gttgagggaa gtgtgacggt tgcgacggcg
     1381 atgctcgacc agggagcggg gacgcatacc gcgatgcggc tcgtcgcagc ggaagaattg
     1441 aagctgcccc tcgagcgaat ccgtatcagg actctcgaca cgagcgaggt gggaccggat
     1501 accggcatcg gcgccagccg cggcacgcgc atattcggca acgcgactcg actcgcggcg
     1561 gcggaagcga gagagcagtt gctcgaagcg gcgagccgca aattcgagac acccaaacag
     1621 cagatcgagt tgcgcggcga aggcacaatg caaactgccg caggccatag cataagttat
     1681 ggcgagctcg ccgcattttg cggcggagaa gttcgcggtc atggctttta ccgaaatttc
     1741 aactccgggc ccgaggccgc attgtgtatt caagtggctg aagtcagcgt cgacgtcgaa
     1801 accggtcaga tcaagctacg acagtttacg acggcgcaca gtactggaac gattctcaac
     1861 ccgttgatgc atcaagccca gatcgatggt ggtgtcgtga tgggcatggg ctattctgtc
     1921 atggagggca tgtcgatcga cgagggaaag gtaacgacga caaatttcgg cgaaaacaaa
     1981 attccttcat ttcgtgatat tcctcctctc aaaaccgttg ttcaggagtt ccccgtcggc
     2041 aatggtccgt atggcgggat gagcatcggc gagccgccgg tggttccaac ggccgcagct
     2101 atcgccaacg ccgtacacga tgcagtcggt gtgcggatca aggatttacc gattactgcg
     2161 gaaaaagtgt tgaatgccct gaaagcgctc acgcaaaggg tgacatagtc gtcagtaaca
     2221 aacggcgaca gtgcgaccag caaattccga cgatgatggt gcgagtgttg ctgtgactag
     2281 cgcgcgctga tcacttggga aatggcacga taactgagag gttttttgaa acagacccta
     2341 atgccggatt cctccagatc cggtgggact ggctcactcc cacgcatacc gatcacgaat
     2401 attccaggaa ttctcgtttg cgcatagttc agaattgttt tccagttcgc catgtccaga
     2461 tccaggagca agaccccggc gtccgtgttg ctattgatcg caaagagcgc tgtttcggca
     2521 ttcgcttcga aggctcgatg gccatcgcag cgtaacaaat ccgcgagcgc ttcgcgggca
     2581 cgcgtataac tctcggcaac gattactccg gtgggtagag aaggtggtag taaatcttgt
     2641 ttcaagagag ctccatcgct cacatatttg cgaatgcata gagcaacggc tgtgccgtcg
     2701 atggagcgaa tcggccaacc aaaatgaaag agaaaatgat gttgcattct cgtgaatgca
     2761 aaaaacggca gaagactttg gcaacgctgc aatttgtcgg acaatgcgga caaattaata
     2821 atggtcaatt cggaatcggc ggcgtttagc gccggagtta gatttcggcg tgctcgatgg
     2881 gcacaatggg ccaggcgacg acttcctgta agcgggagaa atggcaaaga ggttggctgt
     2941 tggatagctc gatccccgag gcctggagca tcgtacagct gcgcagttcc accgtcgcag
     3001 gttgcaagcg ccattgtgga tggtgaatct cgccgaacac gatgcgatcg ggcctgagcg
     3061 cgctaaaaag gcagtatctt tccgtgagcc aatgatccaa tgtccccgtc tgagccatat
     3121 aagtttctcc tgtaggcgca tacgttgcat cgaactccgc tgctgcggct ttcttgtgat
     3181 cgcgcaagct tttgtaatca acggattcgc ccgctgatcc cactttcatc cgcgcatcgt
     3241 aatagggcat gcccagccag cgcgctgctc tgaccgcgag ccaattggcc gcatccaggc
     3301 tgaagaacca aacaccggtc ttgtgcggtg tcttcacgta agtgcggacg ttcagctcgg
     3361 gaaaagccaa agggatcgga aggtagtgcg gtcgaacacc ggtcatgcga aacggcacga
     3421 tgccgatcca acaccagccg tcgaaagtat ccaattccaa gaccctcggg atcagaggcc
     3481 gaattgtctc cgggcgtacg ggccagtgaa ggaagagaag atcgtgccag cgcatgaaca
     3541 gaaaccatgc tgtttctggc agcggccaag ttcgatgcga cgtctgctcg atgagcggcg
     3601 cttcgttcac tgcgcaaccg gcgatcaagc tcgccgatgc cgtagcacct gtgttcaact
     3661 caagaggttc gcgccgggtt aacattgaga tcgcagctcg ctgggccgcc gcctgaaagg
     3721 tgagcctaat cttttgccgt ctcaccccaa gaacgtcggg atatccggta cttcacatag
     3781 attcatgttg cgcgcgtttg tgcccacagt tttgagtcta atcattggat ctgatactga
     3841 gtgtacagca ttatgttcac gagtttgagc aaatgaaaaa cgagcttgct caacgattgg
     3901 tgcgaggtct caaggaggcg ggaatcgatt ttgtgaccta cttgcccgag acacgcttga
     3961 gccagatcgt gcctttgatt cgtgacgatc cagccatgga attgattgcg gcctcgagcg
     4021 aacaagaggc ggtaaccatc gccgtcgggg cgacactagg cggaaggcag ggcgccgttt
     4081 atatggagaa cacgggcgtc tatgtttcga gctacagttt gcttgtcacg ggcaagcagc
     4141 tcggcgtgcc gattcttctt gtgattggtt atctcggcgg tgttcccgat cagcgtaaca
     4201 gttttctcta tgcgaccatc ggcaagcaca cggttccggt gctcgaaggc ttgggcatcg
     4261 ggtatcaaat tatcgaagac ggtcagaagc tcgaaaacaa aattcgcgat gccgtgaggg
     4321 ccgccaatgc gcagcgggcg ccttacgcgc tgctgtttgc cggggagttt tccgaatgat
     4381 tcggcatgca tgtctcgaac tgctcgcggg gaaaatgact gatcagctgg tggtcacgtc
     4441 gcagagcggt cagcgtatcg agtggtcgca tgtgtcgaaa cacgaaggca atttactcct
     4501 cggcatgatg ggctgcgcaa tcggcgtcgc caccggcctg gccctcgctc tgccgcaccg
     4561 aaaagtgatt gcgctcgact ccgatggcag cgtactgctg tcgcttttca atctcgcgac
     4621 tttgggcaat ctgcggccga aaaatcttgt ggtgtatgtt ttcgataacg gagtctacag
     4681 cggcagccga atcagttatc ccacggccac tgcgggaaac actgatttgg cggcaatggc
     4741 gcgcgccgcc gggatctcga acgcttgcac gatcactgag atcagtgagt ttgaaagcaa
     4801 aggaatcggt gcgttgacat ccaacgagct cggttttttt gtctgcaaag tcgaggagag
     4861 cttactccac cgcgagatcc ctcggccgaa cacagatctc gccgagaaca aatatacttt
     4921 tgttcgatat cttgaacgta cagagggcaa aaccatttca ttcgtcgggc gcggatgaaa
     4981 tttgagagga agacaacgtg aagaagcgat ttctattttt cctggcaacc ctagctctgg
     5041 cgatcaatgt tcagtctatc gcccaggggg aggatt
//
LOCUS       DASULZ010000156         9115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_830852, whole genome shotgun sequence.
ACCESSION   DASULZ010000156 DASULZ010000000
VERSION     DASULZ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9115
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830852"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFY96_03165"
     CDS             <1..324
                     /locus_tag="VFY96_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5605486.1"
                     /translation="SAYTASKHGLLGLTRALALETAQMGVTVNAICPGYVDDERTREN
                     AEIMANTTGKTVHEIVNVFAGSAPQHRLIAPAEVASLAVLIASEQLGGMTGQAINVDG
                     GAVMI"
     gene            355..1506
                     /gene="acd"
                     /locus_tag="VFY96_03170"
     CDS             355..1506
                     /gene="acd"
                     /locus_tag="VFY96_03170"
                     /EC_number="1.3.99.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaryl-CoA dehydrogenase Acd"
                     /protein_id="HEX5605487.1"
                     /translation="MDFELSDELIAARDLAREFAEKEIEPSAAKDDREKNFRGDLVKK
                     MGELGFYGTMIPDLYGGNGLGFLAMVLITEEIARAHSAMRVAINMQIGPAVTILQFGT
                     EEQRRKFIPPLLSGEKIGCFAITEADAGSDVAGMRTIAKRDGDRYLLNGSKIFITNAT
                     VSQGGLVYAYTDRSQKHRGMSAFYADWDQPGLSRKPIETLGALSSPIGELIFENFPVP
                     AANRLGNEGDGFKICMWQLNQTRLNCAAGALGLARAAKEAAVNYCNQRVQFGQKIGEF
                     QMNQDLIAQMIAHEEAARLLVYRAAWLADQQKPNNLETSIGKYAAAEAANFAADAAMK
                     ILGAYGYSTEFPVERYYRDAKSYQIVEGSSNIQKLIIAQDALGYRKANR"
     gene            complement(1510..2292)
                     /locus_tag="VFY96_03175"
     CDS             complement(1510..2292)
                     /locus_tag="VFY96_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5605488.1"
                     /translation="MNGIHAENLSFSYRDRPVLHGISLSISGGEMVGILGPNGSGKTT
                     LLKIFSGVLRGQGDLKIKGKNIEQYKRPELSRVFAVVQQAAPVNFPYSAAEIVLMGRA
                     SHHSPLALENHHDLEIARSSMDLTDSLDFASRYLHELSGGEKQRVMIARALAQEPEIL
                     LLDEPSAFLDLKHQIQVFELLLRLNRERGLTIVAALHDLNLAALFFPRLVMLCNGQIH
                     RDGAPEEVLTDKIIQDVYGIRVRIEHDSATKKPQLFIRPSLS"
     gene            complement(2289..3320)
                     /locus_tag="VFY96_03180"
     CDS             complement(2289..3320)
                     /locus_tag="VFY96_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX5605489.1"
                     /translation="MKVLDRRKMILVNSGLLILLVLTAAACTLLGPVRLDLSDALKNI
                     FSNTADAEILLRARLPRILLGIAIGGGMAACGVVLQALLGNPLACPQMLGVSGGASLG
                     GILGLIFFPNSVLPGGNLGEISWIPLAAFLGALVSMVLIYRFSLFQGRLHPYHLLLSG
                     VILNSFIAAVIMFLNSIVDFYQAQGLLFWLMGSLSTRAYLTVGCIWLYILLGFLWLWS
                     KGLALNVITLGEDAAMHLGIAPGSLQRQIFIASSLMIGAMVSVSGMIGFVGLMVPHVM
                     RLLIGSDHRLLLPASFLAGGIFLCWADTLARVLVAPAELPVGIVTAFFGGPFFLFLLY
                     REGRKTLAL"
     gene            complement(3301..4212)
                     /locus_tag="VFY96_03185"
     CDS             complement(3301..4212)
                     /locus_tag="VFY96_03185"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HEX5605490.1"
                     /translation="MIRRPRLIALELRLFGLFFLMALSSSGNAAAADFPRRIVSLAPS
                     VTETVFALGFGNRLVGVTARCDYPAEAKSLPKVGDFMSPSLEVIAAKQPDLVIGVAGA
                     TDPAKAREIERLGIKITLVSVSSVNQILSSFKRIAGLLGDPDAGVGLVETITSQFDRV
                     KKRVAPALRRSTLLTVGLRPLVAVGGKNFLDELITLAGGANIAGDASQPWLNLPDEYV
                     LAKAPQVIIEAGMGSDAAALAKHWSDLKSIPAVKQRRVYSYSSDKILRPGPRIGEGLE
                     EIARLIHPECFDHANSAKGTRCEGARS"
     gene            complement(4209..4934)
                     /gene="cobS"
                     /locus_tag="VFY96_03190"
     CDS             complement(4209..4934)
                     /gene="cobS"
                     /locus_tag="VFY96_03190"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEX5605491.1"
                     /translation="MSSFFAALRFLTIFPGPVRLAAADWRHAAIFFPVIGLLLGMILV
                     LVDFPLRLLASPGLCGALLVSILAFLTGGLHLDGLSDTFDGLGAGGDRERMLTVMDDS
                     HVGALGMIAVLLVLILKIEAIQSTDTDRWRVLLAAPILSRWAMVLLGYRSLAAKPGLG
                     SSLIDQLETKHVVLASLVSVILVIAILRIAGIALLLLTVLFTWLMRTYFHRRLGGIPG
                     DVFGAVSELTETFALVFLALGAR"
     gene            complement(4931..5986)
                     /gene="cobT"
                     /locus_tag="VFY96_03195"
     CDS             complement(4931..5986)
                     /gene="cobT"
                     /locus_tag="VFY96_03195"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEX5605492.1"
                     /translation="MLLEETLDQIKPLSASCDAAAQRHLDSLTKPQGSLGRLEELARR
                     VAVIQEAIPPRLGAKLIFVFAADHGITEERVSAYPKEVTAQMTQNFLDGGAAINVLAR
                     HYQIKTEIVDVGVDHDFAQCRNLRHCKLRRATANFSRGPALTREQARASVELGIRLVK
                     QSAPEHLFLLGAGEMGIGNTSSAAAIICALVGAAPAKIVGRGTGIDDETLARKITAVE
                     NGLRINSPDANDPLDVLAKVGGLEIGAITGVIIAAAAFRLPVVVDGFISGAAALVAQR
                     LSPHIRDILFASHLSAEKGHRLVLDELHLDPLLDLGMRLGEGTGACLAMGLIEASVKV
                     SREMATFASAGVNEKLS"
     gene            complement(5977..6513)
                     /gene="cobU"
                     /locus_tag="VFY96_03200"
     CDS             complement(5977..6513)
                     /gene="cobU"
                     /locus_tag="VFY96_03200"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEX5605493.1"
                     /translation="MAKEIILITGGARSGKSRYAEGRAAALGARRLYIATAEPKDEEM
                     ARRIDEHKLRRRSQWLTVEEPVELCSVLLAQCGRIDAALVDCVTLWLSNLILREDENL
                     VEHRVRDLLEILPRLDFHALFVTNEVGSGIVPENALARGFRDLAGSVNQRLAAVAGEV
                     VFMVTGIPLIVKKAASCS"
     gene            complement(6521..6652)
                     /locus_tag="VFY96_03205"
     CDS             complement(6521..6652)
                     /locus_tag="VFY96_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605494.1"
                     /translation="MPAPVAGPASDFLGLGSAYFRIDIKQRQENRRLIAALVEWLRS"
     gene            complement(6683..8272)
                     /locus_tag="VFY96_03210"
     CDS             complement(6683..8272)
                     /locus_tag="VFY96_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEX5605495.1"
                     /translation="MERKNALMILGTASHVGKSIITTGLGRIFAEDGYRVAPFKAQNM
                     SLNSAATFDGGEIGRAQALQAQACRVAPCVEMNPVLLKPSTDTGAQVILLGKIWGQVS
                     ASDYHTRRVEQLFPEVLSAYKRLSSNYDLVLLEGAGSPAEINLREHDIVNMRMAHGAD
                     ATCLLVGDIDRGGVFASLLGTLELLEPADRASIGGFVINKFRGDESLLSPGVKMIEKR
                     LGLPCVGVVPFIHDLGLDEEDGVALEDRVSAARRWNNVESGSARALRVGVIALPHMAN
                     FTDFDPLARESGISLAYIQHPEELADADLLILPGTKQTLDDLRWLKHHGFARELSRLS
                     ELGVPIIGICGGFQILGTTIQDPHGIENDGVPISADGLGLLPIRTVLRKDKTVRRVRG
                     LLRGNFFRASRSRHVPLEGYEIHVGETLYQAGARPLADIERQGVREAVPDGAVSQSGR
                     VFGTYVHGFLDCDEFRHSFIQAARAAADLAPAAAFLNSSAEREARIDRWACHLRKSLN
                     MNLLKSWIVGPRHRAAENSTG"
     gene            complement(8298..8606)
                     /locus_tag="VFY96_03215"
     CDS             complement(8298..8606)
                     /locus_tag="VFY96_03215"
                     /inference="COORDINATES: protein motif:HMM:NF025833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEX5605496.1"
                     /translation="MIEVTVTIDFGPAERSPLEVVVAVPEKSTVLDVLLQRLPVTTYP
                     RYGMDHFVEAIDGVKNDFVNDRGWRFDVNGRGSNVAAERYLVKHGDWIKWQYRAGSCR
                     "
     gene            complement(8650..>9115)
                     /locus_tag="VFY96_03220"
     CDS             complement(8650..>9115)
                     /locus_tag="VFY96_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605497.1"
                     /translation="PFGCFRAENVGIARFDGAEWTLAIQLVESVSLNANYTYLDWDTA
                     DGRLPRRSRNRGNVTLNYIHDEWNVNLSANLVGRRDDFRAASPFGDITKPGYAIFNLA
                     SYYNLPWRVPVVKDLTLFGRVENLFNRKYEEIDGFRARPLNFLVGMRATFRS"
BASE COUNT         2182 a         2558 c         2610 g         1765 t
ORIGIN      
        1 agcgcatata cggcttcgaa gcatggacta ctcggcctga cccgagcgct cgcacttgaa
       61 accgcgcaaa tgggagtcac cgtaaacgcc atctgccccg gctatgtcga tgatgagcgc
      121 acgagagaga acgcagagat catggcgaac acaaccggca aaacggtgca cgaaatcgtt
      181 aatgtttttg ccgggagcgc gccacagcac cgcctgatcg caccggcgga agtcgcctcg
      241 cttgcggttc tcatcgcttc ggaacagctt ggtgggatga ccgggcaagc gatcaatgtg
      301 gacggcggcg ccgtgatgat ttgagtgtgg cggagcatcg gaagaggagc gcgtatggat
      361 tttgaattat cggacgagtt aattgcagcg cgcgacctgg cgcgcgagtt cgctgagaaa
      421 gagatcgagc ccagtgcggc gaaggacgat cgggaaaaga actttcgcgg cgatctcgtc
      481 aaaaagatgg gcgagctcgg cttctacggc acgatgattc ccgatctcta tggcgggaac
      541 ggacttggat ttcttgcgat ggtgctcatc actgaagaga ttgcgcgggc gcacagtgcc
      601 atgcgcgttg ccatcaacat gcagatcggt cccgcggtta ccatcctgca gttcggcaca
      661 gaggaacagc ggcgcaaatt tattccaccg ttgctttcag gcgagaagat cggttgcttc
      721 gcaataaccg aggccgatgc cgggtcggac gtggccggta tgcggacgat cgcgaaaaga
      781 gacggcgatc gctatctgct caacggcagc aagattttta tcaccaacgc aaccgtgagc
      841 cagggcggtc tcgtctatgc gtacaccgat cgttcgcaga agcaccgcgg catgtcggca
      901 ttttatgccg attgggatca gccgggtcta agtcgcaagc cgatcgagac attgggcgcg
      961 cttagctcgc cgatcggcga actgatcttc gagaattttc cggttcccgc ggccaatcgg
     1021 ttgggcaatg aaggcgacgg tttcaagatc tgcatgtggc agctcaatca aacccggctc
     1081 aattgcgcgg cgggcgcgtt gggtctggcg cgcgccgcca aggaagcggc ggtgaattat
     1141 tgcaatcaga gagttcagtt cggccaaaaa atcggcgagt ttcagatgaa ccaggacctg
     1201 atcgcgcaga tgatcgccca cgaggaagcg gcgcgattgc ttgtctatcg agcggcctgg
     1261 ctcgccgatc aacaaaagcc caacaatctg gaaacttcca ttggcaagta tgctgcggcg
     1321 gaagcggcga actttgcggc cgacgcggcg atgaagattc tcggcgcgta cggctactcg
     1381 accgagtttc ccgtcgagcg ctactatcgc gacgcgaaat cctaccagat cgtcgaaggc
     1441 tcttcgaata ttcagaagct gatcatcgcg caggacgcgc tgggatatag aaaagcgaac
     1501 cggtgacgat cacgatagag aagggcgaat aaacagctgc ggtttttttg tcgcgctgtc
     1561 gtgctcgatg cggacgcgta tcccgtaaac atcctgaatg atcttgtctg tgagcacctc
     1621 ctcgggtgct ccgtcgcgat ggatttgccc attgcagagc atcaccaggc gcgggaaaaa
     1681 caatgccgct aaattgaggt cgtggagggc ggcaacgatg gtgagaccgc gttcgcggtt
     1741 gagccggagt aatagctcaa acacctgaat ttgatgcttc agatcgagaa aagccgaggg
     1801 ttcatccagt aaaagaattt ccggttcctg tgccagggcg cgggcaatca tcacgcgctg
     1861 cttttcgccg ccgctcagct cgtggagata acgactggca aaatcgagcg agtcggtgag
     1921 atccatgctg gagcgagcga tctcaaggtc gtgatggttc tcgagcgcca gagggctgtg
     1981 atgactggcg cgccccatga ggacgatttc tgcggcgctg taggggaaat tgaccggcgc
     2041 tgcctgctgc acgacggcga acacgcggct cagttcgggt cgtttatatt gttcaatatt
     2101 tttgcccttg attttaagat caccttggcc gcggagcact cccgaaaaga ttttgagcag
     2161 ggtggttttc cccgaaccgt tgggaccgag gatgcccacc atttccccgc cagatattga
     2221 gagtgagatg ccgtgcaaca cggggcgatc tctgtagctg aaagagagat tctctgcgtg
     2281 aatgccattc ataacgccag ggtcttgcgg ccttcccgat agaggagaaa gagaaaaaaa
     2341 ggcccgccga aaaaggccgt gacaattccc accggtaact cggccggcgc gaccagaacg
     2401 cgagcgagag tatcggccca gcaaagaaaa atcccaccgg caaggaacga tgcgggcagc
     2461 aacaaacgat gatcgctgcc gatgagcagg cgcatgacgt ggggcaccat caaaccaaca
     2521 aagccgatca tgccgctgac agaaaccatc gcgccgatca tcagcgacga agcaataaag
     2581 atttgacgct ggagagagcc cggtgcaatg cccaaatgca tggctgcatc ctcgcctaat
     2641 gtgatcacgt tcaatgcaag ccctttgctc cagagccaga gaaaaccgag aagaatgtat
     2701 agccagatgc aaccgacggt cagataggcg cgcgtcgaca ggcttcccat caaccagaaa
     2761 agcaatccct gagcttgata gaaatcgacg atggaattga gaaacataat gacggcggcg
     2821 atgaaggagt tcaaaatcac ccctgataac agcagatggt agggatgcag gcgtccttga
     2881 aataaggaaa accgatagat cagaaccatg ctgacgagcg cccccaaaaa agcggcgagc
     2941 ggaatccagg aaatttcacc gaggtttcca ccaggcaaaa cagagttagg aaaaaagatc
     3001 aggcccaaga tgccgcccaa cgacgcgcca cccgacacac cgagcatttg gggacatgcc
     3061 agcggattac ccagtaaagc ctgcagaaca acgccgcagg ctgccatgcc gccgccaatg
     3121 gcaattccca gcaggatgcg ggggaggcgt gcgcgcaaaa gtatttccgc gtctgcggtg
     3181 ttagaaaaaa tattttttag ggcgtctgac aagtcgagtc gcaccggccc gagcagagta
     3241 caggccgcgg cggtaagaac aagcaggatc aacagaccgc tgttgacgag aatcattttt
     3301 ctacgatcga gcaccttcac agcgggttcc tttagcggag ttagcgtggt cgaagcactc
     3361 cggatgtatc agccgagcga tctcttccaa tccttcgccg atgcgtgggc ctggccggag
     3421 tatcttatcc gagctgtagc tatacacccg cctttgtttt actgccggaa tcgattttag
     3481 atcgctccag tgtttcgcca gggcggcggc gtccgaaccc ataccggcct cgataatcac
     3541 ttgtggagct ttcgcaagca cgtactcgtc cggcagattg agccagggct gagacgcatc
     3601 gccggcgatg tttgcaccgc cggcaagagt aatcagctcg tcaagaaagt tttttccgcc
     3661 gacagccacc agcggtctaa gtcccactgt caaaagcgtc gagcgtcgca gtgctggcgc
     3721 aacgcgcttt ttcactctgt cgaactgcga cgtgatcgtc tcaaccagcc ccactccagc
     3781 gtccggatcg ccgagcaaac ccgcgatacg cttaaacgag ctcaagatct gattgacgct
     3841 cgacaccgaa accaaagtta ttttgatacc gagccgttcg atttctcgcg ctttggcggg
     3901 atcagtcgcg cctgcgacac cgatcaccag atccggctgc ttggcggcaa tcacttcaag
     3961 actcgggctc atgaaatcgc cgactttagg caaacttttc gcttcggctg gataatcgca
     4021 gcgagcggtg acgccgacca agcgattgcc aaagccgagc gcgaagactg tctcggtgac
     4081 gctgggagca agggagacga tgcgccgagg aaaatctgcg gccgcggcat tgccggagga
     4141 agacaatgcc atcaaaaaaa acaagccgaa caggcgcaac tctaaggcaa tcagtcgtgg
     4201 ccggcgaatc atcgcgctcc caaggccaaa aagaccagcg caaaggtttc agtcagttcg
     4261 ctcacggcgc cgaagacgtc gccgggaatg ccgcccaaac ggcgatgaaa ataggttctc
     4321 atgagccacg tgaaaagcac cgtcaaaaga agcagcgcaa ttccggcgat gcgtaggatg
     4381 gcaatgacta agatgaccga aaccagactt gcaaggacga catgcttggt ttcaagctga
     4441 tcgatcagag acgaacccaa tcccggcttt gcggcgagag aacggtaacc caacagcacc
     4501 atggcccaac ggctgagaat cggcgcggcg agcaggacgc gccagcgatc ggtatcggtg
     4561 ctttggatag cctcgatttt gaggataagc accaacagga cggcgatcat acccaacgcg
     4621 ccgacgtggc tgtcgtccat aacggtcagc atgcgctcac gatcaccacc agcgccgagg
     4681 ccatcgaatg tgtcactgag cccgtccaga tgaaggccgc ccgtgagaaa agccagaata
     4741 ctcacgagaa gcgccccgca tagcccggga cttgccaaca gcctcaaggg aaaatccacc
     4801 agcactaaaa tcattcccaa cagaagtccg atgacgggaa agaagattgc ggcgtgtcgc
     4861 caatccgcgg cagcgagcct cacaggaccc ggaaaaatag ttaagaaccg taacgctgca
     4921 aaaaaggaac tcatgagagt ttctcattta cgccggcgga cgcaaaggtt gccatctcgc
     4981 gagaaacttt taccgatgct tcgatcagtc ccattgcgag acacgcgccg gtgccctcgc
     5041 caaggcgcat gcccaaatcg agcaaaggat cgagatggag ttcgtcaagc acgagccgat
     5101 gtcctttttc agcagataag tgcgaggcga agagaatatc tcgaatgtgc ggcgacaatc
     5161 gctgtgccac aagcgccgcg gcgccggaaa tgaagccatc gacgacgacg ggcagccgaa
     5221 atgccgcggc ggcgataatg acaccggtga tggctccaat ttccaagccg ccgaccttcg
     5281 caagcacgtc gagaggatcg ttggcatcgg gagagttgat gcgaaggcca ttctcgacgg
     5341 cggtaatctt ccgagcaaga gtctcgtcgt cgatcccggt ccctcgccca acgatctttg
     5401 ccggcgccgc gcctaccagt gcgcagatga ttgccgcggc gctactggtg ttgccgatgc
     5461 ccatttcgcc ggcgccgagc aaaaacaagt gttcaggtgc cgattgtttg acaagtcgaa
     5521 tgccaagctc tacggaagcg cgcgcctgct cgcgagtcag cgccggtccg cgggaaaaat
     5581 tggctgtcgc gcggcgtaac ttgcagtggc gcaaattgcg gcattgggca aagtcatgat
     5641 ccacgccaac atcgacaatt tcggttttga tttgatagtg acgcgctaga acattgatcg
     5701 ccgctccgcc atcgagaaag ttctgggtca tttgcgcggt gacctctttg ggatatgcgc
     5761 tgacccgttc ctcggtgata ccatggtcgg cggcgaagac gaatatcaac ttggctccga
     5821 ggcggggtgg aattgcctcc tgaatgacgg ccactcggcg cgcgagctcc tcgagacgcc
     5881 ccaagcttcc ttgcggcttt gtcaacgaat cgagatggcg ctgcgcggca gcgtcgcacg
     5941 aggcgcttag gggttttatt tgatcaaggg tttcctctag gagcatgacg cggccttttt
     6001 tacgatcagc ggaatgccgg tgaccatgaa aacaacttcg ccggcaacag ctgcaaggcg
     6061 ctgattgacc gagcccgcta aatcgcgaaa gccgcgcgcg agcgcatttt ccggcacgat
     6121 ccccgatccg acctcgttag tgacgaacag cgcgtgaaaa tcgaggcgcg gtaatatttc
     6181 gagcagatcg cgcactctgt gctcgacgag attttcatct tcgcgaagaa taagattgct
     6241 cagccaaagg gtcacacaat cgaccaaggc cgcgtcgatt cggccacact gcgctagcaa
     6301 aacggagcat aattccaccg gctcttcgac cgtcagccac tgactgcggc ggcgcagctt
     6361 atgttcatcg atgcggcgcg ccatttcttc gtctttcggt tccgcagtgg cgatatagag
     6421 tcgccttgca ccgagggctg cggcacgtcc ttcggcgtac cggctctttc ccgagcgcgc
     6481 gccgccggtg atcaaaataa tttctttggc catgagcctt ctacgacctc agccattcga
     6541 ccagcgccgc gatcaggcgg cggttttctt gccgctgttt gatgtcgatg cggaaataag
     6601 cgctgccgag ccccaagaaa tcgcttgccg ggcccgctac aggtgccggc aatgccaaac
     6661 gcgaaattgg atttcatgtc gatcagcctg tggaattctc cgctgcccgg tgacgtggac
     6721 cgacaatcca gcttttgagc agattcatat tcagcgattt tcgaagatga cacgcccagc
     6781 ggtcgatgcg tgcttcgcgt tcggcgctgc tatttaaaaa agcggccgca ggggcaagat
     6841 cggcggcggc acgcgcggct tggataaaac tatggcgaaa ttcgtcgcag tccaaaaagc
     6901 cgtgcacata ggtgccaaag acgcggcctg attgactcac ggcgccgtcg ggaacggctt
     6961 cccttactcc ctgacgctca atatcagcga gaggacgtgc tccggcttga tagagcgttt
     7021 ccccgacgtg aatctcatag ccttcaaggg gaacgtgccg agatcggcta gcgcgaaaga
     7081 aattgccgcg cagaagaccg cgaacgcggc gcaccgtttt atctttgcgc aatacagttc
     7141 gtatgggcag cagaccgaga ccgtctgcac tgatggggac gccgtcattt tcaatcccgt
     7201 gagggtcttg gatcgtggta ccgagaatct gaaaaccgcc gcagatgccg atgatcggga
     7261 cgccaagttc gcttaggcga gaaagctccc gggcaaaacc atggtgtttg agccaacgca
     7321 ggtcatcgag ggtttgcttg gtgccgggaa gaatgagcag atcggcgtcg gccagttctt
     7381 cagggtgctg tatataagcc agcgatattc ccgactcgcg cgccaaagga tcgaagtcgg
     7441 tgaaattcgc catgtgcggc agagcaataa cgccgacgcg gagcgcccgc gctgaccctg
     7501 attcgacatt gttccagcgg cgcgccgctg atacgcgatc ttccagcgcg acaccatctt
     7561 cttcgtcgag gccgagatca tgaataaacg gcaccacgcc aacgcaaggc aagccaaggc
     7621 gcttttcgat cattttaacg ccaggcgata gcagggactc atcgccgcga aacttgttga
     7681 tgacgaagcc gccgatagag gcgcggtctg caggttccaa cagttccagg gtgccgagca
     7741 acgatgcgaa aactccgcca cggtcaatgt cgccgaccaa gaggcaggtg gcatcggcac
     7801 catgcgccat acgcatgttg acgatgtcgt gttcacgcaa gttgatctcg gctggcgagc
     7861 cggcgccttc gaggagcaca aggtcgtaat tactcgacaa gcgtttgtag gcgctcagca
     7921 cttccggaaa aagctgctcg acgcggcgcg tgtgatagtc ggatgcgctg acctgtcccc
     7981 atatctttcc cagcaaaatg acttgggcgc cggtatcggt cgacggcttc agcaaaacag
     8041 gattcatctc cacgcatggc gcgacgcgac aagcttgcgc ttgcagcgcc tgggcacgac
     8101 cgatctcacc gccgtcaaac gttgctgccg agttgagcga catgttctgc gccttgaacg
     8161 gcgcgacgcg atagccgtcc tcggcgaaga tgcgccccaa accggtcgtg atgatcgact
     8221 tgccgacatg cgaggccgtc ccaagaatca tcaaggcatt cttgcgttcc attggattca
     8281 cttctgttcg ctcaggttta acggcaagac ccggcacggt actgccactt gatccaatcg
     8341 ccatgtttta ccagataccg ctcggcggcc acgttagatc ctcgaccgtt gacatcgaag
     8401 cgccagccgc ggtcgttaac gaaatcgttt ttaactccgt cgatcgcctc gacgaaatga
     8461 tccatgccat atctgggata agttgtgact ggcaatcgct gtaacaaaac atcgagtacg
     8521 gtactctttt cgggcacggc gaccacgact tccaagggcg atcgctccgc gggtccaaag
     8581 tcgatggtca ccgtgacctc gatcatacat tcctcggatg attctgggcg tgccggaaat
     8641 ccggttgact cagctgcgaa acgtcgcgcg catgccgacc aaaaaattca gcggccgggc
     8701 gcgaaaaccg tcaatctcct cgtatttgcg attaaacaaa ttctccaccc tgccgaacaa
     8761 cgtcagatct tttaccaccg gcacgcgcca aggcaaatta tagtaggaag cgagattgaa
     8821 gatcgcgtaa cccggtttgg tgatatcgcc aaatggtgac gcggctctaa aatcatcgcg
     8881 ccggcccacc agattggcgc tcagattcac gttccactca tcatggatat agttcagtgt
     8941 cacattgccg cgatttctcg agcggcgcgg aagcctgcca tcggcagtat cccaatccaa
     9001 ataagtgtaa ttggcattta ggctgacgga ttcgacaagt tgtatagcca aagtccactc
     9061 ggcaccatcg aaacgcgcaa tgccgacatt ttcggcgcga aaacaaccga aagga
//
LOCUS       DASULZ010000157         3355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_526695, whole genome shotgun sequence.
ACCESSION   DASULZ010000157 DASULZ010000000
VERSION     DASULZ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526695"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VFY96_03225"
     CDS             <1..186
                     /locus_tag="VFY96_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605498.1"
                     /translation="IDFEQTEGFFKGKTWKGIYVLEGDTLRICDNAPDLARDRSAAFE
                     AKSKSAYALITFTRTNA"
     gene            825..1616
                     /locus_tag="VFY96_03230"
     CDS             825..1616
                     /locus_tag="VFY96_03230"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX5605499.1"
                     /translation="MIEVYHNDMSVCAQKVRFALGEKALQWESRHLNLRAGDQQKPEY
                     VKLNPNAVVPTLVDGGNVIIESTVICEYLDDAYPEPSLSPRDPVERARMRLWTKQLDE
                     SVHAATRTISNAIAFRHQKLALGTEALKALHDKIPDPKKRAESWENVDKGVDSSYLAD
                     AARRFEKLLSDMESTLSSSPWLAGNSFSLADIGYAPYILRLKDLQLQFLWDKRPYIAA
                     WFEKLGERPGYKTAFDDWPNESYAVLMREKGIEAAPRIKAVLGLA"
     gene            1815..2093
                     /locus_tag="VFY96_03235"
     CDS             1815..2093
                     /locus_tag="VFY96_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX5605500.1"
                     /translation="MIEIRQTESYANWFDSLRDRKARARIDVRIRRLSLGNPGDVRRV
                     SELRIDYGPGYRVYFVQRGPMLIILLAGGDKRTQERDIKRALELARRQ"
     gene            2102..2422
                     /locus_tag="VFY96_03240"
     CDS             2102..2422
                     /locus_tag="VFY96_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013209163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein"
                     /protein_id="HEX5605501.1"
                     /translation="MRKKTAKIKTRPWDPAEHLTTEGDMAAYLEAALEDGDPALIAAA
                     LGDIARAKGMSQIARETGLGRESLYKALSPDGNPEFSTVLKVVRALGLRLHAVAPAKS
                     SAAR"
     gene            2553..3251
                     /locus_tag="VFY96_03245"
     CDS             2553..3251
                     /locus_tag="VFY96_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605502.1"
                     /translation="MLLLTNEDVEQVLTMADCVGVLRRFFEEESKGRVLTRQRTESWL
                     PHLTTDTFYQCKTMEGGVPYVGKYVIRIDSNVTRKKQQDLSSRIEHVPFGNGSWMGVL
                     LVFSTDTGELLGWMPDGYLQRTRVGALYALAADYLARPDAEEVGLIGSGWQAGGQILG
                     LRCVRNIKKVRVFSSNADRRSRFAEELQASLASKCALSVPRGMRSPTRTSSHSRPTPT
                     RRSWSLPGSSPGSI"
BASE COUNT          758 a          881 c         1031 g          685 t
ORIGIN      
        1 attgacttcg agcaaacgga aggattcttc aaagggaaga cgtggaaggg aatctacgtc
       61 ttggaaggcg acacgctgag gatctgcgac aacgcaccgg acttggcaag ggaccgatcg
      121 gccgcgttcg aggcgaagag caagtccgca tacgccctca tcacgttcac gcgtacgaat
      181 gcttgaacga acccgggcga agttgtaaat gacacaacgc ctcgaccgtt gagagatcga
      241 ggtattcttt ttttcgggat gaagatgctt acacgacggc agtagcgggg cttgctctaa
      301 agggataagc acgatgatgc tagttggcga gtgtaatcaa tgcggcgcct gctgtttcac
      361 caaatccggc gcagtctgtg agaacctcga aattttgact cggcaaggct tgcccaacgc
      421 tactcggtgc agggtctact ccgaacgata cgacggtttg ccaatccgaa tgattgccaa
      481 agacggaaaa attttaagag ggtattactg cgcgaaaaac tcgccggccg aagtgtcagt
      541 gatcatcgag atgggtatca aaaagggtgt gtgctcgctg agtcgcgttg accaggcaga
      601 tcggaccggg cgaaagcagc cctcggcgca agaggcataa tgtgggttcg gtgatgagtt
      661 agggtgcgta gggcgcggcc gataccgcac tgtcgctcgc cgctggagcg atcgtcgaga
      721 tgtgaaagtc tgagccccgg agctgaccga gttgccagag tcgtacccgc ccgattgacc
      781 ggatcgagtt ttggcagggg ataaaatcgt agtaggatcg gcgcatgatc gaagtttatc
      841 ataacgatat gtccgtgtgc gcgcagaagg tgcgctttgc gctcggcgag aaggcgcttc
      901 agtgggaaag ccgccatttg aacttgcgcg ccggcgacca gcagaagccg gagtacgtca
      961 agctcaatcc caacgccgtg gtgccgacgt tggtggacgg cggcaacgtc atcatcgagt
     1021 ccacggtgat ctgcgaatat ctcgacgacg cctatccgga accgtcacta agcccgcgcg
     1081 atccggtcga gcgggcgcgc atgcggctgt ggaccaaaca gctcgatgaa agcgtccacg
     1141 ctgcaacccg aacgatcagc aacgccatcg cgtttcgcca tcagaagctc gccctcggca
     1201 ccgaggcctt gaaggcgctg cacgacaaaa ttcccgatcc gaagaaacgc gcggagagct
     1261 gggaaaatgt cgacaagggc gtcgattctt cgtatcttgc cgatgccgcg cggcgtttcg
     1321 aaaagctgct gtcggacatg gaatcgacgc tgagttcgtc gccgtggctg gcgggaaaca
     1381 gtttttccct ggccgatatc ggctatgcgc cgtacatttt gcgcctgaaa gatctccagc
     1441 ttcagttcct gtgggacaaa cgaccttata tcgccgcctg gttcgagaag ctcggcgagc
     1501 ggcccggcta caaaaccgca ttcgacgact ggcccaacga atcttatgcg gtcctgatgc
     1561 gagaaaaggg gatcgaagcc gcgccgcgca tcaaagcggt tctcgggttg gcttgagcgg
     1621 cgggccgagt tgccggcaat cacgcctgac gtgtcgcatg actccacgcg agatcgcgaa
     1681 aagctacgat catctcgccg atcgcctggc tcttagagcg agttttctca atcaactgct
     1741 gcaaggtttc cttcccttgg ctattgagcc ggcgcttgac ggattgtatc ttataaaata
     1801 cactctccat cagagtgatc gaaatccgtc aaaccgaaag ttacgccaat tggttcgaca
     1861 gtttgcgtga tcggaaagcg cgggcgcgaa tcgatgtgcg tatccgtcga ttgtcgctag
     1921 gtaatccggg agatgtaaga cgagtctcgg aattgcggat cgattacggt cccggctatc
     1981 gcgtgtattt tgtgcaacgc ggtccgatgc tgatcatctt gcttgccggc ggtgataagc
     2041 gaacgcagga gcgggacata aagagagctt tggaattagc acgaagacaa tagaggttgt
     2101 catgagaaag aagactgcaa agataaaaac tcgaccgtgg gacccagccg agcatttgac
     2161 cacggagggc gatatggcgg cgtacttgga agccgcactc gaagacggcg acccggcact
     2221 tattgccgcg gcactgggcg acatcgcacg tgccaaggga atgagccaga ttgcccgcga
     2281 aacgggtcta ggacgagaga gcctttataa agcgctatca ccggacggca acccggaatt
     2341 ctccacggtg ctcaaggtgg tgcgcgcgct cggcctccgc ctgcacgcgg tagcaccggc
     2401 aaaatcctcg gcggcaaggt gatgcgggtg ctcaggagaa tggttcgcta gatccgagaa
     2461 aatgtcaaaa ggcaaagact tgaccgtgca ggttcagact cgttgtcttc accattttac
     2521 gtttgggcac ggcgattcga agaggtgata acttgttgct gttgaccaac gaagacgtgg
     2581 aacaggtttt aacgatggcc gactgcgtcg gtgtgttgcg ccgtttcttt gaagaggaaa
     2641 gtaaaggccg tgtgttgacg cggcagcgca cggaaagctg gctgccccat ctcacgaccg
     2701 atacattcta tcagtgcaag acgatggaag gcggagttcc ttacgttggc aagtacgtca
     2761 ttcggatcga ttccaacgtc accaggaaaa agcagcagga tctgtcgagc cgcatcgagc
     2821 atgttccctt cggcaacggg agctggatgg gtgtcttgct tgttttctct accgatacgg
     2881 gcgaactgct cggctggatg cccgacggct atctccagcg gacccgtgtg ggagcgttgt
     2941 atgccctcgc tgccgattac ttggcgcgcc cggatgccga agaagttggg ctgatcggct
     3001 ccggctggca ggctggcggg cagatcctcg gattgcgttg cgtgcggaac atcaagaagg
     3061 tgcgggtttt tagttccaat gctgatcggc gcagccgctt cgccgaagag ttgcaggcga
     3121 gcttggcatc gaagtgcgcc ctgtcggtgc cgcgcgggat gcgttcgcca acgcggacat
     3181 cgtcgcactc gcgaccaacg ccaacgagaa ggtcatggag tcttcctggg tcgagcccgg
     3241 gcagcatctg agctccgtgc gctttcttga gctcgatccc cgcgcttacg aagactgcga
     3301 ccgcgtggtg gtcaaccgag tcgaaccttg gatacaaaac tatcatctcg gtgaa
//
LOCUS       DASULZ010000158         2739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_729063, whole genome shotgun sequence.
ACCESSION   DASULZ010000158 DASULZ010000000
VERSION     DASULZ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729063"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VFY96_03250"
     CDS             <1..919
                     /locus_tag="VFY96_03250"
                     /inference="COORDINATES: protein motif:HMM:NF018449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HEX5605503.1"
                     /translation="KLLYSTTFGGRTARAKAIALDLKGNVYIAGTSAGDLPTTPGAFQ
                     PKPRGQLDGFVAKLSTTFNDTIGVFRPSGNAFLLRNSNTAGPADLNVPFGIAGDLPVT
                     GDWDGNGIDDVGIFRPSTKQWLLRLQNGTTITLNAFGQNGDLPVAGDWNGDGIDTIGI
                     FRPTTNEWILSNGPNTNNTSPPENLHIPFGDNGDIPITGDWTGAGFDSVGTFRPSNSL
                     FILSNGFSSTVDITAFFGVPGVRPFVGDWDGDGVTTIGVFNPATGTMSLNNTNTSGNG
                     VGDIVFSFGLNGDSPLAGDWDGKAEVPVN"
     gene            1021..2169
                     /locus_tag="VFY96_03255"
     CDS             1021..2169
                     /locus_tag="VFY96_03255"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEX5605504.1"
                     /translation="MRSFYEFFAGGGMVRAGLGGGWECLLSNDFDHKKARVYRENWGG
                     GPELKVENISLLETSDLPGRANLAWASFPCQDLSLAGAGAGLKGERSGTFWPFWDLIK
                     GLIAEQRAPEIIVLENVCGTMTSHGSTDFATICAALCDGGYAVGAVVIDAILFVPQSR
                     PRLFFIAVRRDVKVPAAQINLTPTQPWHNKTLTTAYERLSDEIKENWIWWNLPLPRRR
                     RLCLTDILEEDPTSVTWFSAQETERLLSLMSEVNQAKLVEAKQSGRRIVGGVYKRTRR
                     DEYGRKAQRAEIRFDDVAGCLRTPAGGSSRQVIVVVESASVRARLISSRETARLMGLR
                     DSYKLPRNYNEAYHLTGDGVVVPVVRHLRKFIFEPLLTYALTQARRAA"
     gene            2180..2578
                     /locus_tag="VFY96_03260"
     CDS             2180..2578
                     /locus_tag="VFY96_03260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HEX5605505.1"
                     /translation="MEVSPLRHRTMQAVKSKNTTPELIVRRLLHTNGYRYQLHRADLP
                     GCPDIVFSRKKKIIFIHGCFWHGHSCARGARIPKSNTEYWTSKISRNRDRDAVVKKKL
                     KANGWAQVVLWECETKNESAILKKISRFLN"
BASE COUNT          661 a          674 c          756 g          648 t
ORIGIN      
        1 gaagctgtta tactcgacga cgtttggtgg acgaacggcg agagccaaag caatcgcgct
       61 ggacttgaaa ggcaatgtgt atatcgctgg tacgtcggct ggagatctac cgactacgcc
      121 cggagcgttt caacccaaac cgcgagggca gttggatggg tttgtcgcta aactctcaac
      181 tacgttcaat gacaccatcg gcgtatttcg tccttcgggc aacgcttttc tacttcgcaa
      241 ctcgaatact gccggcccgg cagatctcaa cgttccgttc ggaatcgcag gggaccttcc
      301 ggttacaggc gattgggacg gcaatggaat cgatgacgtt ggtattttta gaccaagcac
      361 gaaacagtgg ctattgcgtc ttcagaatgg gaccacaatc acgctgaatg cctttggaca
      421 aaatggtgat ctgccagtgg ctggtgattg gaatggcgac ggcatcgaca cgatcggcat
      481 ctttcgaccc acgacgaatg agtggatttt gagcaatggg ccaaacacga acaatacgtc
      541 gcctccggaa aaccttcaca ttcccttcgg cgacaacggt gatattccaa ttaccggtga
      601 ttggaccggc gccggtttcg actcggtggg aaccttccga ccaagcaact cactattcat
      661 tttgagcaat ggattcagta gcaccgtcga cataacagcc ttttttggcg taccgggagt
      721 acggcctttc gtcggagatt gggatggtga tggtgtcaca accattggcg ttttcaatcc
      781 agccaccgga acgatgtcac tcaacaacac gaatacgagt ggcaatggtg tgggagacat
      841 tgttttcagc ttcggactca acggcgattc tccgttggcc ggagactggg atggaaaagc
      901 cgaagtaccc gtgaattaac tgaggaaaag agggcgtcta tcaaacgacg ccctttcgtt
      961 tcacttcgtg gttgagatac tcttttcctg tttgtctcat tccaattgag cgcgtcgcaa
     1021 atgcgtagct tctacgagtt ttttgccggg ggaggaatgg ttcgagcggg gctcggcggc
     1081 ggatgggaat gcctgttgtc aaatgatttc gatcacaaga aggcgcgcgt atatcgggaa
     1141 aactggggag gcggtccgga actaaaggtc gagaatattt ccttactaga aacaagtgat
     1201 ctgcccgggc gagcgaatct ggcgtgggcc tcttttcctt gtcaggatct ctctcttgcg
     1261 ggtgctggag ccgggttaaa gggagagcga tcaggcacgt tttggccatt ttgggatctc
     1321 atcaagggat tgattgcaga gcagcgcgcg ccggaaatca tagtgctcga gaatgtctgc
     1381 gggacgatga cttctcatgg cagtacagac ttcgccacga tttgtgctgc actttgcgac
     1441 ggcggttatg ctgttggcgc agtcgtgatc gatgcgattt tattcgtgcc gcagtcgagg
     1501 ccgcgactat ttttcatagc tgttcgccgc gacgttaaag ttccggccgc gcagattaat
     1561 ctcacgccta ctcaaccctg gcacaacaag acgctgacaa ctgcttacga aaggttaagc
     1621 gacgaaatca aggaaaattg gatatggtgg aacctcccgc tgccacgccg ccgccggcta
     1681 tgcttaacgg acatacttga agaagacccg acaagcgtaa cctggttctc agctcaggaa
     1741 accgagcggc tgctttcgtt gatgagcgag gtaaaccagg ccaagctcgt tgaagcaaaa
     1801 caatcaggcc ggcgcatcgt tggcggcgtt tataaaagaa ctcgccgcga cgagtacggc
     1861 agaaaagctc agcgcgcaga gatacgcttc gatgatgtgg ctggatgttt acgcactcca
     1921 gcgggcggtt caagccgcca ggtgatagtc gtggtggaaa gtgcttcagt acgagctcgg
     1981 ttgatctcca gtcgtgaaac cgctcgcctc atgggcttac gcgacagtta caaattgcct
     2041 cgtaactata atgaggctta tcacctgacc ggagacggag tggttgtgcc ggtcgttcgg
     2101 caccttcgaa agttcatctt cgaaccgctg ttgacctatg cgctgacgca agcgagacgc
     2161 gcggcatgag cataccctga tggaagttag cccactcaga caccgaacga tgcaggcggt
     2221 gaagagtaag aacacgacgc cggaactgat cgtccgcagg ttattacata caaacggtta
     2281 tcgatatcag ctacatcgcg ctgatctgcc gggctgcccg gacattgtat tttctcgcaa
     2341 gaagaaaatt atctttatcc atggttgttt ttggcatggg cacagttgtg ctcgaggcgc
     2401 gcgcattccc aagtccaaca ccgaatattg gacatcaaag atctcgcgaa atcgcgatcg
     2461 agatgctgtg gtgaagaaga aattgaaggc aaatggttgg gcgcaggttg tgttgtggga
     2521 gtgcgaaaca aaaaatgagt ctgcaatatt aaaaaagatt tccaggttct tgaactgatt
     2581 tctggtgatt agcgtgtacc aggtttttca cgcctttgcg tgaactcctt gtttgaccgc
     2641 ttctaaattt gacacccacc acgaatcgca ttatcgtatc gcccgctcca aacccgacaa
     2701 gtagacctcc caaaaagccc agccgtattc ggactgtct
//
LOCUS       DASULZ010000159         3933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_526747, whole genome shotgun sequence.
ACCESSION   DASULZ010000159 DASULZ010000000
VERSION     DASULZ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526747"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1053)
                     /locus_tag="VFY96_03265"
     CDS             complement(64..1053)
                     /locus_tag="VFY96_03265"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605506.1"
                     /translation="MIRPIVIFALLVGACCPSGARAQERVKIAYSSADASNTVWFTAL
                     DAGIYKKYALDVELIFIQSSTMSVSTVVSGDIQVANASGGAVASAAVSGANLVMTACY
                     INTLPYELIVQESVKSPEDLKGKSVGISRVGSASDVAARVLIKGLGLEPVRDVPILQV
                     GGPTERAAAFRTGRIVGFPSPPGTIYLAKGMPHRIMISTADFQKRYEFPYICSTTTKN
                     YLASHRDTIRRLTMALIESTHFLKTHKEETKKFIAKYTRQDNPQYLEASYAANVKLHD
                     RVPLVTREGTEVQIKEALARKPGASWRVEEIVDDSIVRELEKSGFIDKVYNTR"
     gene            complement(1134..2234)
                     /locus_tag="VFY96_03270"
     CDS             complement(1134..2234)
                     /locus_tag="VFY96_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5605507.1"
                     /translation="MIEVKSLTKIYADGDNAGVKAVDNISFKVEEGRFYTLLGPSGCG
                     KTTTLRCIAGLEKGNAGEITVAGKKVYSANDNTFVSANRRPIGMVFQSYAIWPHLSVF
                     ENVAFPLRVGKQRFSSSDIRQKVSDALAQVELGGYEERMATQLSGGQQQRLALARALV
                     REPQVLLLDEPLSNLDAKLRERMRFELRELQRRLRITTLYVTHDQIEALSMSNVIAVM
                     NAGVIIQEGGPRDIYLQPKSKFVANFIGSTNQLSGRVARLDADGTGAVKTENGEISCQ
                     VLEGLPVGSNVVVVVRPESINLHVDRPASSLNVIEGKIGAAMFLGEYVDCAVELGKQM
                     LQTHQRYTVQVRRGDQVWVELPAAECMALPAE"
     gene            complement(2309..>3933)
                     /locus_tag="VFY96_03275"
     CDS             complement(2309..>3933)
                     /locus_tag="VFY96_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX5605508.1"
                     /translation="VLPLLMLVVGSFQAETAPREFIYTLKNYQAAYASPYTYSTFMNS
                     VIFASGSAILSFSLGTMLAWLTERTNTPLRSIFVPLAVVPLILPGVLEAIAWIFLLSP
                     KFGYLNVWLMQLFGLKSPPFNVFSLPGMIWVHSVGQVPLAFLLMVAAFKSMDPSLEES
                     AMMSGAGTWQTFKRITLRLLMPTTASVLLILFVRTLESFETPALIGIPSRIYVYTSEI
                     YLAFNDYPPDYGRGGALSVGLLLLSAVGVWLYTRGTKEGKKFQTVTGKAFRPRQFDLG
                     KWRWLGLGFLLTYFVFVVLMPFLVLFWASFLPFFATPGWDAVSKLSFDNYRYLGTFRP
                     FWDALQNSLILATLSATVAMILTSLVAWIVYKSRLPGSWLLDFLAFVPITIPGIVMGM
                     ALILLYVAFPLPIYGTIWMLLIAYVTRYIPYGMRAASGSIMQIHGELEEAAAASGASW
                     WESFKRVTLPLLRPGLVAGWIYICIVSFREFSTSILLATGESRVLSILLFTMFEQGQV
                     TVVAAIGMLMIATLLAIVAVFYKLTGRVGIQT"
BASE COUNT          924 a         1148 c         1055 g          806 t
ORIGIN      
        1 atgaaggggg attttgtttt cgctcgaaat aacccgcatc acttcatcat ttcggacggc
       61 aactcaccga gtgttataga ccttgtcgat gaaaccgctt ttttccagtt cccgcacaat
      121 actatcatcc acaatctcct cgacccgcca tgacgcaccc ggttttcgcg ccagtgcttc
      181 tttgatctgc acctcggtac cttcgcgcgt gaccaatggc acacggtcgt gcagctttac
      241 attggcggca taggaggctt ccaagtattg cggattgtct tggcgcgtgt atttggcgat
      301 gaattttttt gtctcttcct tgtgagtttt gaggaaatga gtcgactcga tgagcgccat
      361 cgtgagccgg cgaatggtat cgcggtggct cgctagataa ttctttgtcg tagtcgagca
      421 gatgtaggga aactcataac gcttttgaaa atccgccgtg ctgatcataa ttcgatgcgg
      481 catgcctttg gctagataaa tcgtgcccgg tggcgaggga aacccgacga tccgtccggt
      541 gcgaaacgcc gccgctcttt ccgtcgggcc acctacctgg agaatcggca catcgcgcac
      601 cggctccaac cccaagcctt tgatcagtac ccgcgcagcc acatcggaag cgctgccgac
      661 tcggctgatg ccaacgcttt tccctttcaa gtcctccggt gatttgaccg actcctggac
      721 gattaattcg tacggcagcg tattgatata gcaggcggtc atcaccaggt tcgccccgct
      781 cactgcggcg ctcgccaccg caccgcccga agcatttgcg acctgaatgt cgcctgagac
      841 cactgtggat acgctcatcg tcgagctttg aatgaagatc agctccacgt ccaaagcata
      901 ttttttatag atgccggcgt cgagcgccgt gaaccagacg gtgttcgagg catccgccga
      961 gctataagcg attttgacgc gctcctgcgc tcgagcaccg ctcggacagc aggcgccgac
     1021 tagtaatgca aaaatcacga tagggcggat cattgctttg acctcctcat gcctgatcca
     1081 gacgcgcctc ccgagttaaa agggagctag ggaaattcgg ctcgatctcg cgatcactcg
     1141 gcaggtagtg ccatacactc ggcggcggga agctcgaccc atacctgatc gccacggcgt
     1201 acttggacgg tgtagcgctg gtgagtttgt aacatctgtt tgccgagctc cacggcacag
     1261 tcgacatatt cgcctaaaaa catggccgcg ccgatcttgc cttcgatgac gttcaggctc
     1321 gacgccggcc ggtcaacgtg gagattgata ctttcgggac gaaccaccac gacgacattg
     1381 ctacccactg gcaagccctc gagaacctgg catgaaatct cgccgttctc ggttttcacg
     1441 gcgccggttc cgtccgcgtc gagccgcgcc acacgcccgc tcagctggtt cgtcgaaccg
     1501 atgaagttcg cgacaaactt gctcttcggc tgtagataga tgtcgcgcgg cccaccttcc
     1561 tgaatgatca cgccggcgtt catcaccgcg atcacgttcg acatggacag agcttcgatc
     1621 tgatcgtggg tgacgtacag agtggtgatt cgcaagcggc gctgcagctc tcttagctcg
     1681 aagcgcatgc gctcgcggag cttcgcgtcc aaattgctca aaggctcatc gagcagcaac
     1741 acctgcggct cgcgcaccag cgcgcgcgcc aacgcgagcc gctgttgctg cccgccggaa
     1801 agctgcgtcg ccatgcgctc ttcataaccg ccgagttcca cttgtgcgag tgcatcgctc
     1861 accttttgtc gaatatcgct actgctgaaa cgttgcttgc cgacccgcag tggaaaggcg
     1921 acgttctcaa atacgctgag gtgcggccaa atggcgtagc tttggaaaac catgccgatg
     1981 ggacgacggt tagccgatac gaacgtgttg tcgttggcgg aatagacctt tttgccggcg
     2041 acggtaattt cccctgcatt ccccttttca aggccggcga tacaacgcaa tgtggtcgtt
     2101 ttgccgcagc cggaaggacc caagagagtg taaaagcgtc cttcctcgac cttgaacgag
     2161 atattgtcta ccgccttgac tccggcatta tcgccgtcgg cgtaaatctt cgtgagggat
     2221 ttgacttcaa tcatggactc gactcgagct gttcagcgcc ggatccgacg aattgaacag
     2281 cttgaacggt ttgaacgggt tcaaagccct atgtttgtat tccaacccgg cctgtgagtt
     2341 tgtagaacac ggccacaatc gccagtaggg tcgcgatcat cagcatgccg atcgcggcca
     2401 cgacggttac ttgaccttgc tcgaacatag taaacaacaa gatggagagc acccggcttt
     2461 cccccgtcgc gagcagaatc gaagtcgaaa actcccggaa ggaaacgatg cagatataaa
     2521 tccagcccgc caccagtccc ggtcgcaaaa gcggcagggt cacgcgcttg aaactctccc
     2581 accaggaggc tcccgacgcc gcggcggcct cttccagctc accatggatt tgcatgatgg
     2641 aaccggaagc cgctcgcatc ccgtaaggga tgtagcgcgt cacgtacgcg atcagcagca
     2701 tccaaatcgt gccgtaaatg ggcagcggga atgccacgta cagtaggatc agcgccatac
     2761 ccataacgat cccaggaatc gtgatcggca cgaaggcaag aaaatcgagg agccatgacc
     2821 caggcaaacg gctcttgtag acgatccacg cgaccagcga agtcaatatc atcgccaccg
     2881 tcgcggaaag cgtcgccaaa atgagactgt tttgcagcgc gtcccaaaac ggccgaaaag
     2941 tcccaagata acgataattg tcgaagctca acttgctgac ggcgtcccaa ccgggggttg
     3001 caaagaacgg cagaaatgac gcccaaaaca ggaccaagaa cggcatcagc accacaaaga
     3061 caaaataggt tagcaaaaag cctaggccga gccagcgcca ttttcccagg tcgaattgcc
     3121 tagggcgaaa tgcctttccg gtaacggttt gaaatttctt cccttccttc gtaccgcgtg
     3181 tgtaaagcca cacgccgacc gcgctcagaa gcagtagccc cacggaaagc gcgccgccgc
     3241 ggccataatc cggtggataa tcattaaaag ccagatagat ttcactggta tagacgtaga
     3301 ttcgcgacgg tatgccgatc aaagccggcg tctcaaacga ttcgagcgtg cgcacgaaca
     3361 aaatcaacag caccgatgcc gtcgtcggca tgagtaagcg aagcgtgatg cgcttgaagg
     3421 tctgccaggt ccccgcgccc gacatcatcg ccgattcttc cagggacgga tccatcgatt
     3481 tgaaggccgc gaccatcaat aaaaaagcca gcggcacctg accgacggaa tgcacccaaa
     3541 tcatgccagg cagcgagaag acgttgaacg gcggtgattt caagccgaac agctgcatca
     3601 gccaaacgtt caggtagccg aattttggac tgagcagaaa gatccaggcg atcgcttcaa
     3661 gcacaccggg caagatcaac ggcacaaccg caagcggaac aaaaatgctc cggagcggtg
     3721 tatttgtccg ctcggtgagc cacgccagca tggtgccgag ggaaaagctc aatatcgccg
     3781 agcccgaggc gaaaatcacc gaattcatga aggtggaata cgtataggga ctggcgtagg
     3841 cggcttggta gtttttcagc gtgtagatga actcacgcgg cgctgtctcc gcttgaaagc
     3901 tgcccacgac cagcatcagc aacggcagta ccg
//
LOCUS       DASULZ010000160         2897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_932282, whole genome shotgun sequence.
ACCESSION   DASULZ010000160 DASULZ010000000
VERSION     DASULZ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932282"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFY96_03280"
     CDS             <1..345
                     /locus_tag="VFY96_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605509.1"
                     /translation="GADAALVVRPIQVERKTEYGPSYFPIPWFGYYGPHFGASWYGGY
                     GAPSVYHYNEYTSETTLYDIAKNEVVWSGTIRTADPDNVQSAIKSYVTAVMHALSEKN
                     LLAKPRQGSAPM"
     gene            544..1623
                     /locus_tag="VFY96_03285"
     CDS             544..1623
                     /locus_tag="VFY96_03285"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX5605510.1"
                     /translation="MDRKTDDLRIKDIKELVTPIELLNEFPVTPKAADTVWETRQGIH
                     RILHGAEDRLLVIIGPCSIHDVKAAVEFAERLHETKRGLAADLLMVMRVYFEKPRTTV
                     GWKGLINDPDLDGSYRINDGLRVARRLLRDLNELGMPVGCEFLDMITPQYISDFVAWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGNIRIAVDAIRAAQAPHHFMAVTKAGRS
                     AIASTTGNEDCHVILRGGRQPNYDAENVAAAARTLAQAGIPARLMIDFSHGNSGKDPQ
                     HQIVVAHDVAAQVAGGDETIFGVMMESHLKGGRQDLLPGKPLMYGVSITDACAGWEES
                     RAVLDALAEAVRQRRLKAALDTEDE"
     gene            1661..2659
                     /locus_tag="VFY96_03290"
     CDS             1661..2659
                     /locus_tag="VFY96_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605511.1"
                     /translation="MISQPMHMPLLLLATVFMSAPSFAGAAASAVKLVMTSGSASERD
                     GVIYVAQDLGLFRKHGLDLTFVQVRNGPVAMSALSSGETQFHWGSVSGANLGAIAEGA
                     DLVFVAGFINRLSGMFVVNPQIKTPADLKGRSLAVNSLSGGGWIFSMLMLDYWGLSPE
                     RDRIQFRTLGDQAVMAQGVLSGTADGAFLGYTFGKMLQSKGFRVLADSEKLPIPYQGS
                     GVIAKRSFVTASPASLENLLRALLESAAFIRHPENKAQVTKSLARALRFKRAEDAEDG
                     YHSMLTLYERKIYPSVEGIRNVIRLLGMSNEKIRRLKAEDLVDETVVRKLEKEGRF"
BASE COUNT          659 a          739 c          858 g          641 t
ORIGIN      
        1 ggtgctgatg cagcgctggt tgtccggccc attcaggtgg agagaaagac cgagtacggt
       61 cccagctatt ttccgatccc ctggtttggg tattacgggc cacatttcgg cgcgagctgg
      121 tacggcggat acggagcacc gagcgtctat cattacaacg agtacacgtc ggaaacgacc
      181 ttgtatgaca tagctaaaaa cgaggtcgtt tggtcaggca cgattcgaac cgccgatcct
      241 gataacgttc aaagcgctat taagagctat gtaacggcgg tcatgcacgc gctcagcgag
      301 aaaaatctgt tagcaaagcc caggcaggga tcggcgccga tgtgagcact acgagagatg
      361 tcgtgagcgc aggggtttct gggatcgact aaagcatctt tattctcgct ggtccgttcc
      421 gcaccggtgt ttttggtttt cagccgtcac ttcttcttac ctcatgcttg tcgactgcgt
      481 tcgcgttcga atgcacttta gcctatatga ataaaaagct ttccagcctc gaatggtgcg
      541 gcagtggatc gtaaaactga cgatttgcga atcaaagaca tcaaggaact cgtcactccg
      601 atagagttgc tgaacgagtt tccggtaaca cccaaagctg ccgatacagt ttgggagaca
      661 cgtcaaggca ttcatcgaat tcttcatggt gccgaagatc ggttactggt aattatcggg
      721 ccatgctcaa tccacgacgt caaagccgcc gtcgagtttg cggaacgatt acatgagaca
      781 aagcgcggac tcgccgctga cctactgatg gtgatgcggg tttacttcga aaagccacgg
      841 accaccgttg gctggaaagg tttgatcaat gacccggacc tcgacggcag ctaccgtatc
      901 aacgatggcc tccgagttgc gcgccgattg ctgcgcgact tgaacgagtt gggaatgcct
      961 gtcggatgtg aattcctcga catgatcacg ccgcaataca tcagcgattt tgttgcatgg
     1021 ggggcgatcg gggcgcgcac gactgaaagc caagtgcatc gcgagctcgc ttcgggtttg
     1081 tcgtgcccgg tcggattcaa aaacggcacc gacggcaata ttagaattgc cgtagatgcg
     1141 atccgcgccg ctcaggcgcc gcaccatttc atggcggtga ccaaagccgg ccgctctgcg
     1201 atcgcatcga cgacgggaaa tgaggattgt cacgtgattt tacgtggcgg caggcagccc
     1261 aactatgatg ccgagaacgt tgcggctgca gcaagaactc tagcacaggc gggaatcccc
     1321 gcgcgcttga tgatcgactt cagccacggt aatagcggca aggatcccca acatcaaatc
     1381 gttgtcgcgc acgatgtcgc ggcgcaggtt gcgggcggcg acgaaaccat ttttggcgtg
     1441 atgatggaaa gtcatttgaa aggcgggcgg caggatttgc tccccggtaa gccattgatg
     1501 tatggcgtaa gcatcaccga tgcgtgcgca ggatgggagg aaagccgcgc cgtcctggat
     1561 gctttagcgg aggccgtgcg tcaacgccga ctcaaagcgg ccttggacac tgaagacgaa
     1621 tagcacgttt tgcgaacgag cgttcacaaa ggagctctgc gtgatttctc agcctatgca
     1681 tatgccactt ctgctcttgg cgactgtttt tatgagcgcg ccttcgttcg ccggagcggc
     1741 ggcgagcgcc gtcaaactcg tgatgacttc cgggtcagcg agcgagcggg acggggtcat
     1801 ctatgtcgcg caggatctgg gcttgttccg caaacacggc ctcgatttaa ctttcgtgca
     1861 agtgcgcaat ggtccggtgg cgatgtcggc gcttagctcc ggcgagacgc aatttcattg
     1921 gggctcagtc tcgggagcca atctgggcgc gattgccgag ggcgcggatc tcgtgtttgt
     1981 cgctggattc atcaaccgtc tctcaggaat gtttgtcgtc aatccgcaaa tcaaaacgcc
     2041 ggccgatttg aaaggacgaa gcctggccgt caacagtttg agtggcggcg gttggatctt
     2101 ttccatgctg atgctcgatt attggggact ttcgccggag cgcgatcgga ttcagttccg
     2161 tacgctcggc gatcaggcgg tcatggcgca gggtgttctg agcgggactg cggacggcgc
     2221 atttttaggt tacacgttcg gaaaaatgct ccagagcaaa ggatttcgcg tgcttgccga
     2281 tagcgaaaaa ctgccgattc catatcaagg atcgggggtc atcgccaagc gcagcttcgt
     2341 aactgcgtcg cccgcgtcct tggagaattt attgcgcgcc cttctggaga gcgcggcgtt
     2401 tattcgccat ccggagaaca aagcgcaggt gaccaaaagc cttgcccgtg cgctccgctt
     2461 taagcgcgcc gaagatgccg aagacgggta tcacagcatg ctcactttgt acgagagaaa
     2521 aatttatcca agtgtggagg ggattcgcaa cgtcatccgc ctcctcggca tgagcaatga
     2581 aaaaatccgg cgtctcaaag ccgaagattt ggtcgatgag acggtggtgc gaaaactcga
     2641 gaaagaggga agattctgat cacgccagcg gtttaaaacc cagccattat gtcatagagg
     2701 attcccactg ctgctggacg cgagatagat ccacttaact catctgtcct cgttagaagg
     2761 agaagataat gcctttcgaa aagatcggta tcatgagcat cggcgagatg ggctttcact
     2821 gggcgaaact gctcaaggcc catggagtgg aagtattgac gtatgacaag gaccgcggcg
     2881 aggtgagtcg cagacgc
//
LOCUS       DASULZ010000161         3030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_932442, whole genome shotgun sequence.
ACCESSION   DASULZ010000161 DASULZ010000000
VERSION     DASULZ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932442"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..657
                     /locus_tag="VFY96_03295"
     CDS             64..657
                     /locus_tag="VFY96_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605512.1"
                     /translation="MKTRKRIFGILVLAAVALTGCDELARVGDLGNYGSLGSDLVGEV
                     EYVDTRAREIEIRTDSGATSVIRYDDQTQVIYRQRNYSVANLEPGDYIAARVQQDRDG
                     RNYTDTITVREGVQDRGNRRGSTRLDRIEGRIESIDERRGTFELRDSRNRLMVVAVPF
                     NAPRSVIDRFNRLRTGDYVRIEGRAAGAERFDLESFL"
     gene            complement(769..2391)
                     /locus_tag="VFY96_03300"
     CDS             complement(769..2391)
                     /locus_tag="VFY96_03300"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008101960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX5605513.1"
                     /translation="MATPEFVYQDPLPIEKDTTRYRLLTKGYVSVTEFEGHEILKIDP
                     AALTLLANVAIRDVSFLLRTAHLEKVAAILKDPHASSNDRYVALAMLRNADTAAKGIL
                     PFCQDTGTATIVAKKGQRVWTGVKDEEYLARGVYKTYTEENLRYSQTAPLNMYDEVDT
                     GTNLPAQIDIMASDGMEYQFLFVTKGGGSANKTMLYQETKALLNPASLEKFLTEKMKT
                     LGTAGCPPYHLAFVIGGTSAEACLKTVKLATTGYLDHLPTSGNAHGRAFRDVELENRM
                     LEQAYKSGYGAQFGGKYFALDVRIVRLPRHGASCPVGMGVSCSADRNAKAKINREGVW
                     LEELEREPGRLIPEEYRDKIASNVVHVDLNRPMKEILAQLSKYPVTTQLSLTGTMVVA
                     RDIAHAKLKERLDRGEGLPQYMKDHPIYYAGPAKTPEGMPSGSFGPTTAGRMDSYVDL
                     FQSHGGAMITLAKGNRSQQVTDACKKYGGFYLGSIGGPAAILAKENIKKVEVLEYPEL
                     GMEAIWKIAVEDFPAFILVDDKGNDFFKQILQ"
     gene            2704..>3030
                     /locus_tag="VFY96_03305"
     CDS             2704..>3030
                     /locus_tag="VFY96_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605514.1"
                     /translation="MDWIFALLLVLAVPVAATAQTQDQLIAGAKSEGRLVLYASAATQ
                     QLQMYMTAFNKRYPFIKTEYFRTDKQKLVTRVLLEEQTKQRIADLIHTSVIETHILKK
                     RGALSRY"
BASE COUNT          648 a          842 c          809 g          731 t
ORIGIN      
        1 gacaagctcg cccaaaaatt atgaagtaag tcatgatcgc agggatctgt aggaggagtg
       61 tacatgaaaa ctcgcaagcg aatttttggg attttagtct tggccgcggt cgctttaacc
      121 gggtgtgatg aattggcccg cgttggagac ttgggcaatt atggtagcct cggcagcgat
      181 ctggtcggtg aggtagagta cgtcgataca cgcgcccggg agatagaaat tcgcacggat
      241 tccggcgcca catcggtgat ccgttacgat gaccagactc aggtcattta tcgacagcga
      301 aactattccg ttgccaattt ggaaccgggc gactatatcg cggcacgggt gcaacaagac
      361 cgagatggcc gaaattatac cgacacgatc accgttagag aaggcgtcca ggatcgcggc
      421 aaccgccggg gcagcacgcg attggatcgg atcgaaggcc gcatcgagtc catcgatgaa
      481 aggcgtggaa ctttcgaatt gcgcgattcg cgcaaccggc tgatggtcgt cgcagtgcct
      541 ttcaacgctc cccgttcagt gatcgatcgt ttcaaccggc tgcgcaccgg cgattacgtg
      601 cgcatcgagg gacgggctgc aggcgccgag cgtttcgacc tggagagttt tctgtaggag
      661 tggttgcttt tgttgtcgtc gtcatgccgg tgaagaccgt catccaagtt tttccgattc
      721 ctggataccg gcctccgccg gtatgacgtt gagctattgc cataggtttt actgtaaaat
      781 ctgcttgaag aaatcgttgc ccttgtcgtc caccaggatg aacgccggaa aatcttccac
      841 tgcaatcttc cagatcgcct ccataccgag ctcgggatat tcgagcactt cgactttctt
      901 gatgttttct ttggccagga tggccgccgg cccgccgatc gatcccaaat aaaaaccgcc
      961 atattttttg cacgcatcgg tgacctgctg gctgcgattg ccctttgcaa gcgtgatcat
     1021 cgcgccgccg tgcgactgaa acagatcgac ataggaatcc attcgacccg ctgtggtcgg
     1081 cccaaatgat cccgagggca tgccttcggg agtcttcgcg ggacctgcgt aataaatcgg
     1141 atgatccttc atgtactgcg gcaatccctc gccgcggtcg agccgttcct tgagctttgc
     1201 atgcgcgata tcgcgtgcga cgaccatcgt accggtgagt gaaagctggg ttgtcaccgg
     1261 atatttcgag agctgcgcca ggatctcctt catcggtcgg ttgagatcga catggacgac
     1321 attgctcgca atcttgtcac ggtattcttc gggaataaga cgacccggtt cccgctcgag
     1381 ttcctcgagc cagactcctt ctcggttgat tttcgctttg gcattgcgat ccgcggagca
     1441 tgaaacgccc ataccgaccg ggcacgaagc cccatggcgc ggcaaacgaa caatgcgcac
     1501 atccagcgcg aagtactttc cgccgaactg agcgccatag ccggacttgt aggcctgctc
     1561 caacatccga ttttccagct cgacgtcacg aaaagctcgt ccgtgcgcgt taccggaggt
     1621 cggcagatga tcgagatagc cggtcgttgc gagcttcacg gttttcaagc acgcttcggc
     1681 cgacgttcca ccgatcacga acgctagatg atacggggga caaccggcgg taccgagggt
     1741 tttcattttc tcggtcaaga atttttcgag gctcgccgga ttgagcagcg ccttggtttc
     1801 ttgatagagc atcgtcttgt tcgccgaacc gccgccctta gtcacgaaga gaaactgata
     1861 ctccattccg tcgctggcca tgatgtcaat ttgcgccggc aaattcgttc cggtatccac
     1921 ctcgtcgtac atgttgagcg gcgccgtctg cgaatagcgc aggttttctt cggtgtaggt
     1981 cttgtaaacc cctctggcaa gatactcttc gtccttgacc ccggtccaga ctctctgacc
     2041 ttttttcgcg acgatggtcg ccgtcccggt gtcctggcaa aacggtagaa ttcccttcgc
     2101 cgcggtgtcg gcattgcgaa gcatcgccag ggcaacataa cgatcgttgc tcgaagcgtg
     2161 cggatccttg agaatcgccg cgactttttc cagatgcgcc gtacgcaaca aaaaggacac
     2221 gtcacggatc gcgacgttcg ccagcaaggt taaggccgct ggatcgatct tgagaatttc
     2281 atggccttca aactccgtga ctgaaacgta acctttggtc aggagccgat agcgggtggt
     2341 gtctttctcg ataggaagcg ggtcttgata aacgaattcc ggcgtagcca taaacttcac
     2401 ctctctcggc tgaatcctag atgaattttc ctattttgtc gacgcgtcgc tgcgcatcgc
     2461 tttactatca attggaccga agttttttgg ttttgaatcg cagctccccc gccggttcgc
     2521 cgtcgatgtt tcattcagga cgcttctctc ttgtcattcg aaacttcggg cagactcagg
     2581 gtaaactccg tgagaaatgt ttcctctctt cttcctacac tcgcgcctta cggcctcgcg
     2641 ttgtgccgct aaaggttgac agaaccacct caactcacta gtttgccgag gtcgggaggt
     2701 ttcatggatt ggatctttgc tttgcttttg gttcttgccg tgccagttgc agcgacggcg
     2761 caaactcaag atcaactcat cgccggcgcg aagagcgagg gcagactagt actttatgct
     2821 tcggcagcga cacagcaatt acagatgtac atgacagcgt tcaacaaacg ttatcccttc
     2881 attaagaccg aatacttccg cacggacaag cagaaactcg tcactcgcgt gttgctcgag
     2941 gagcaaacca aacagcgcat cgccgatctc attcacacca gcgtcatcga gactcacatt
     3001 ctgaaaaaac gcggcgcgct cagccgctac
//
LOCUS       DASULZ010000162         4606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_527477, whole genome shotgun sequence.
ACCESSION   DASULZ010000162 DASULZ010000000
VERSION     DASULZ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527477"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..908
                     /locus_tag="VFY96_03310"
     CDS             48..908
                     /locus_tag="VFY96_03310"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX5605515.1"
                     /translation="MAIRSFDLLQPRSVPEAVAMLARHGDDARAVGGGTTLVILMKQR
                     ALYYPYLVDLQTIPGLSEIKVESDGVRIGALVTHRALEFSRVIRASFPALADAVGQIG
                     NVRIRQTASIGGNLAHADYRLDPPPALLVLGAEVSLFGADGARTLPLSQFFQGLYETA
                     LKPAELLIDIKVPFMPENSRAVYLRYSTLSANDWPCLGVAAFLSKAKGRCRELRVALG
                     GLASTPVLVGGLDFVNDQTLDSAVIERLLDTVDQQIAPFGDFRGSEWYKRRMACLFVK
                     KAVEQLNSAG"
     gene            920..1396
                     /locus_tag="VFY96_03315"
     CDS             920..1396
                     /locus_tag="VFY96_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5605516.1"
                     /translation="MRREITLNINRRLETLTIDDADTLLEVLSERLKLWSVRESCGVG
                     VCGSCTVLVDGRPVSSCLLLAARAGGEITTLEGLEPGSALHEIQQAFVEERALQCAYC
                     TPGFVLTVKAFLEQNRNPSDQEIREYLAGNLCRCAGYADILQAVRAAQAKINSRQR"
     gene            1596..3527
                     /locus_tag="VFY96_03320"
     CDS             1596..3527
                     /locus_tag="VFY96_03320"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5605517.1"
                     /translation="MLYAHAAKGIQTMEQITTLDSAGRPNRWNLDRLFDAASKDTIIR
                     LRWPLVILSSYLLYYTPSEWFTPTQIQALLALYLLSHSTLYFLADDLFDSPYFYAPLL
                     IFDTVVLLAVFSTSGTASPDFYVACLFTVVVSCICNDARGLLITTLLAPLGYAYIVFH
                     SAADLDPQVYLRLPFPFMISLFYGYFAQVARMRRTARIKDEQVRLQQKAAAEIRRQRE
                     RLEVLHEVNRSMTSTIDQKQILESFLDSALIHLPYAAACVRIRNSANQKLETVAARGF
                     KVSALEHAKELVRWIDGVAESGAPLAVRNVFTDGQTKNRGFFESEGLVSIVALPLAAN
                     GEILGCSVWLTRNEHEFNQEEVDFLSTLVGQAAVAVQHSELYDQSRRQTDELRSAHKI
                     KDEFLKAVSTQLKTPLNVITGYAEMFLHGVLGEMTPIQEKAIETIGRQSKELHRLINT
                     VLQVSNVEAEKLQVELHEFSLWEFFSELRSFYDDGLATKEVQLVWSYPSDLPAVQCDR
                     AKLRRILENLIDNAIKFTERGTIGIVARYMQREKMLQVKVLDTGDGIPQEQIPKIFDR
                     FQQVPGGRATLHGGVGLGLYLVKKYVEVLGGHIQVNSRVGESSSFTLQIPAALDNATD
                     AHQQLLSAPASDAFGADAH"
     gene            complement(3565..4401)
                     /locus_tag="VFY96_03325"
     CDS             complement(3565..4401)
                     /locus_tag="VFY96_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605518.1"
                     /translation="MMSKYRARQCPNCHYFVGFAVAKSFRRGVTASLTNFCLNCNYKL
                     PVHTIVRGNGAVKRRSRRPKLRLIVGTSDSATLIGIVQSQSYTMDTKISPADYARHLR
                     AIGQDLENLHLSSFNLEYVGDAYLVWVRSDSEADANPVFRISKSRLQKLWRNKMPVRP
                     IGHEEPDHRSQSESGKRLRYSVHELDRIEREQRARRRQQSGSADGHRLSQLLRTVGDL
                     VNQRGERLLGIAWQELSVSVVVETPNGRKEIDVFRPDNLYDLWVRMYLKRDNRALLDT
                     PH"
BASE COUNT          999 a         1207 c         1301 g         1099 t
ORIGIN      
        1 taacttgact tagcccgacc gagcaggtta gctgcgaggt tatcgtcatg gcgatccgat
       61 ccttcgatct tttgcagccg cgttctgtgc ctgaggcggt ggcaatgctc gctcgacacg
      121 gcgacgatgc tcgcgcggtt ggcggcggaa cgacattggt gattttgatg aagcagcggg
      181 cgctctatta tccttatttg gtcgacttac agacgatccc cgggttgtct gaaatcaagg
      241 ttgaatcgga cggagtacgg atcggtgctc tcgtgactca tcgcgctctc gagttttcgc
      301 gggtgattcg ggcgtcattt ccggcactcg ccgatgcggt tggacagatt ggaaatgtcc
      361 ggattcgtca aaccgcgtcg atcggtggca acctggctca tgccgattac cgtctcgatc
      421 cgccgcccgc gctcttggtt ttgggcgccg aggtcagcct ctttggcgcg gacggcgcgc
      481 gcactctgcc gctcagtcag ttttttcaag gcttatatga aactgccctg aaaccagccg
      541 agctcttgat cgatatcaag gttccattca tgccggaaaa cagccgggcg gtatacttgc
      601 gctacagcac gctttcggcc aacgattggc cctgtcttgg cgtcgccgcg tttttgagca
      661 aagccaaggg tcgttgtcgt gaattgcgcg tcgcgctggg cggcttggca tccaccccgg
      721 tactcgtcgg cggattggat tttgttaacg atcagacgct cgattccgcc gtcatcgaac
      781 gactcctcga cacggtcgac cagcaaattg cgccgtttgg cgatttccgt ggctcggaat
      841 ggtataaacg gcgcatggct tgcctgtttg tgaaaaaagc ggtcgagcaa ctcaacagtg
      901 ccggctgact tcttgacgca tgagacgcga gatcacttta aatattaatc gccggctaga
      961 gacgctcaca atcgacgatg cggatacgtt gctcgaggtg ctgagcgagc gcttgaagct
     1021 ctggagcgtg cgcgagagtt gcggcgtcgg cgtttgcggc agctgtactg tgctcgtcga
     1081 tggacggccg gtcagttcgt gcttattgct ggcggcgcgc gccggcggcg aaattaccac
     1141 gttggagggg cttgagccgg gctctgcgct gcacgaaatt cagcaagctt tcgtcgaaga
     1201 gcgcgcatta caatgcgcct actgcacgcc cggctttgtg cttaccgtca aagcctttct
     1261 tgaacagaat cggaaccctt ccgaccaaga gatccgggaa tatcttgccg ggaatctttg
     1321 ccgatgcgcc ggctacgcag acattttaca agcggttcgc gctgctcaag caaagatcaa
     1381 ctctcgccag cggtaactct tcacagcgaa aaatcgtttc aacggctgct ctgcgccgag
     1441 gagcatccaa cgacaaatgt gtgcctcgtt gaagcgattt gtgcgtttgg gaagattaat
     1501 ttttagaaga acgcgatcat gcgctacaca accagtcaaa ttgcatagac aatcggcaac
     1561 tcttcgagga cgagccaacg cgaatggttg ctggcatgtt atatgcacat gccgccaagg
     1621 gaattcagac catggaacaa atcacaaccc tcgattctgc tggacgcccg aacaggtgga
     1681 atctcgatcg gctcttcgat gcggcaagca aggataccat tatccgtttg cgctggccgc
     1741 tggtgatcct ttcttcatat ctactgtact acactccgag cgaatggttc acgccaacac
     1801 aaatccaggc gttgttagct ctgtatttgc tgagtcactc gaccttgtat ttcttggctg
     1861 acgatctatt cgattcgccc tatttctacg cgccgctgct gatctttgac actgtcgtgc
     1921 tgcttgcagt gtttagcacc agcggaactg ccagtcctga tttctacgtc gcgtgcctgt
     1981 ttacggtagt cgtgagctgc atttgtaatg acgctcgcgg gttgctgatc actacattac
     2041 tcgccccgct tggatatgcg tatattgttt tccatagcgc ggccgatctc gatccgcagg
     2101 tctatttgag attgccgttt ccatttatga tttcgctctt ctatggctac ttcgcgcaag
     2161 tcgcacgcat gcggcgcacc gcgcggatta aagacgagca ggtaagactt cagcaaaaag
     2221 ccgcggcgga aattcgccgg cagcgggaac ggctggaagt tctgcatgaa gtgaaccgat
     2281 cgatgacgtc gaccatcgat caaaaacaga ttctggaatc attcctcgat tcggcattga
     2341 ttcatttgcc ctatgccgcg gcctgcgttc gaatcagaaa ttctgccaac caaaagctcg
     2401 agaccgtcgc cgcacgtggt ttcaaagtca gcgcgctgga acatgcaaag gagttggtgc
     2461 gctggatcga tggtgtggcg gaatcggggg cacctcttgc ggttcgtaat gtattcacgg
     2521 atggacaaac taaaaatcgc gggttttttg agagcgaggg tttagtatcg attgtcgctc
     2581 tgccgcttgc ggcgaacggt gagatactcg ggtgttccgt gtggctgact cgcaatgagc
     2641 atgagttcaa tcaggaagag gtcgattttt tatcgacgct ggtcggccag gcagcggtcg
     2701 ctgtccaaca ttcagaactc tacgaccaaa gccgccgaca aaccgacgag ctccgcagcg
     2761 cccacaaaat aaaggatgaa ttcttgaaag cggtttcgac ccagctgaaa accccgttga
     2821 atgtgatcac gggatacgcc gaaatgtttt tgcatggggt actgggagag atgaccccga
     2881 tccaggaaaa ggcgatcgag acgatcgggc ggcaatccaa agagctgcat cggttgatca
     2941 atacggtttt gcaagtgagc aatgtggaag ccgaaaagct tcaggttgag cttcatgagt
     3001 ttagcctgtg ggaatttttc tccgagctgc gatctttcta cgacgatggg ctcgccacca
     3061 aagaggtgca gttggtatgg agctatcctt ccgatttgcc agccgtccaa tgcgaccggg
     3121 cgaagctcag gcggattttg gaaaatctta ttgataacgc gatcaagttt acggagcgcg
     3181 gaacgatagg gattgtcgca cggtacatgc agcgagagaa aatgctccag gtcaaggtgc
     3241 tcgataccgg cgacggaatt ccccaggagc agattccgaa aatttttgac cgctttcagc
     3301 aagtgcccgg tggccgcgcg actttgcacg gcggcgtcgg cctgggactc tatcttgtga
     3361 agaagtacgt ggaagttctc ggcgggcaca ttcaggtgaa cagtcgtgtc ggagaaagtt
     3421 cgagctttac tctgcagatc ccggcggcgc ttgacaacgc aaccgacgcg catcaacaac
     3481 tcctctcggc tccggcgagc gacgcttttg gcgcggacgc gcactagacg gcgccggttc
     3541 ttcaggcggt ctaaaaatta agattcagtg tggggtatcg aggagcgcgc ggttatcccg
     3601 ttttaaatac atgcgtaccc aaaggtcgta caggttgtcc gggcggaaca cgtcgatctc
     3661 tttgcggcca ttcggtgtct ctacgacgac gcttacggac aactcctgcc aggcgattcc
     3721 cagaagccgc tcgcctcttt gattgacaag atctccgacc gtgcgcaaca actgagatag
     3781 acggtggcca tccgcgctgc cgctctgctg tcgacgccgc gcgcgctgct cgcgttcaat
     3841 tcgatcgagc tcgtgcacag agtaacgaag tcttttgccg ctctcggatt gcgatcgatg
     3901 gtccggttcc tcgtgaccaa tgggccgcac cggcatcttg tttcgccata atttctgcaa
     3961 acgacttttg ctgatgcgaa agaccggatt ggcgtctgcc tcgctgtccg atcgcaccca
     4021 gacaagatat gcgtcgccca catactccag gttgaaactg gatagatgaa gattctccaa
     4081 atcttggccg atggcgcgca aatggcgggc ataatccgca gggctaatct tagtatccat
     4141 cgtgtaactc tggctctgaa ctatcccgat gagcgtggct gaatcagagg tgccaacgat
     4201 caagcggagc ttcggtcgtc gcgaacgccg cttcacggct ccattgccgc ggacaattgt
     4261 atgcacagga agcttatagt tgcaattgag acagaaattg gtcaaggacg ccgtgacgcc
     4321 gcgccggaag gacttcgcga ccgcaaagcc gacgaagtaa tggcaattgg gacattggcg
     4381 ggcgcggtat ttggacatca tcagtagctc gattggcgag cttttaagtg gaactacggt
     4441 aaaagtcaag caacctcaaa gcactatatg tttaagtgag gaactcttgg cgtcaggaat
     4501 tcatgttttc aatctttctg tcaaatcctg tcacttcttg gaatgaaacg gctattcggc
     4561 gcgcgtccga gggttgtttt ctgggtcggt tgcgagtcca aaggga
//
LOCUS       DASULZ010000163         7158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1033885, whole genome shotgun sequence.
ACCESSION   DASULZ010000163 DASULZ010000000
VERSION     DASULZ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033885"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VFY96_03330"
     CDS             complement(<1..851)
                     /locus_tag="VFY96_03330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5605519.1"
                     /translation="MSKARELTEVEELREQLREAQETLDAIRRGEVDALVVNSPAGDQ
                     VYTLRGADYSARMLLQEMSEGAATLIPDGSILYCNKRFAEMLRLPHEKVIGVLAREFV
                     APVDRPAYDSLIRRGQEYLAKAELLFCASDGTSVPTYCSVNAISIGDEPCLCMVVTDL
                     AEQKRNEEVIAAARLASSILEQAAEIIVVCDSAGRIIQASREADEFWGKSLLGQSFGE
                     VFPLGGLIDSNLKDTTDLLKGCLTGKPIRGVGVNLHRRDGGIVHMLMSAGPLYDKAGS
                     AIGCVVT"
     gene            complement(861..1202)
                     /locus_tag="VFY96_03335"
     CDS             complement(861..1202)
                     /locus_tag="VFY96_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HEX5605520.1"
                     /translation="MASPHKRSRVISFESPLRDRGTDKYILRLYIAGTTPRSTRAVAN
                     IRDICERNLGGRYELEVIDIYQKPALAKGEQIIAAPTLVKQLPLPLRRFIGDLSDTER
                     ILVGMDLAAAS"
     gene            complement(1213..1530)
                     /locus_tag="VFY96_03340"
     CDS             complement(1213..1530)
                     /locus_tag="VFY96_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HEX5605521.1"
                     /translation="MSVTKATKNSETESNKWLLRLYVAGQTPKCIAAFANLKKICAEY
                     LDGHYQIEMIDLLENPQLARGDQIIAIPTLVRNLPEPMRKIIGDLSNTEKVLIGLDLR
                     SRT"
     gene            complement(1533..3272)
                     /gene="kaiC"
                     /locus_tag="VFY96_03345"
     CDS             complement(1533..3272)
                     /gene="kaiC"
                     /locus_tag="VFY96_03345"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HEX5605522.1"
                     /translation="MSKASEPERPALAKSPTGIRGLDEITFGGIPRGRPTLVCGRAGC
                     GKTLFSVEFLIHGAIDYDDPGVFVAFEETEEDLTKNVASLGYDLAELCRRKKLMIDYI
                     RVERSEIEETGEYDLEGLFIRLGHAIDSIGAKRVVLDTIEAIFSGFQNTNILRAELRR
                     LFRWLKDKGVTAVITAEQGDGTLTRHGLEEYVSDCVILLDHRVRDQISTRRLRVVKYR
                     GSSHGTNEYPFLIDEQGISVLPITSLGLEHKATTERVSTGIDSLDQMLGGKGFYRGTS
                     ILLSGTAGTGKTSVAAHFVDAACRRGERCVYFSSEESPAQVTRNLRSIGLDLEPWMKK
                     GLLHFDATRPNFNGLEMHLLRIHKLIDQIKPNIAVFDPLSGYLTLGDSLEVKSMLTRL
                     LDYLKGRQITSIFNALVEGGHSELQSEVGVSSLMDSWILLRNIEHNGERNRGLFILKA
                     RGIAHSNQIREFILTDHGVELKDVYVGTEGVLTGSARAAQEAREKAATQLRREATERK
                     KRELERKRRALDAQIVALRTQFETEAEELQKEIALETTREQALQLDRLQMSQLRSGNG
                     GTKPRRERDGKGA"
     gene            complement(3576..4004)
                     /locus_tag="VFY96_03350"
     CDS             complement(3576..4004)
                     /locus_tag="VFY96_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5605523.1"
                     /translation="MVPRLFPYGIIARITMDNVCASIMPNSILIVDDEENLLVLLNRI
                     LSKEGYAVTVAHGSNQALDLIGKNDFRAAILDIKMFPLDGVALLSEIKRRAPSTRVIM
                     ITAYPTADTRDECFRKGATAYLTKPLDIQKLKGVMRELTN"
     gene            complement(4203..4538)
                     /locus_tag="VFY96_03355"
     CDS             complement(4203..4538)
                     /locus_tag="VFY96_03355"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX5605524.1"
                     /translation="MFLKRDCPRWWKSGASLLLGIFLATSGCTALTGESAGQYVDDST
                     LTARVKSNLVADKVANLTRVDVDTTNQIVSLNGIVESAEQKQRAEQLARQTSGVKGVK
                     NNLQIQGKK"
     gene            complement(4575..4727)
                     /locus_tag="VFY96_03360"
     CDS             complement(4575..4727)
                     /locus_tag="VFY96_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007281962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HEX5605525.1"
                     /translation="MGTILLIILILLLIGALPTWPYSSGWGYYPSGGLGLIVLIIVIL
                     LLTGRL"
     gene            complement(4751..5860)
                     /locus_tag="VFY96_03365"
     CDS             complement(4751..5860)
                     /locus_tag="VFY96_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX5605526.1"
                     /translation="MNSKKPAIKKSPTSYALVLAGGEGKRLKSFIHLLRGDFLPKQYV
                     NFIGRRSMLEHTWDRAEKLMAPSSIFTVVNRSHLAFPDVQRQIAQRSPGTVLVQPENK
                     ETATGIIFALLHIAKRCPNSLVTLLPSDHFILQEEEMMRYLTYAGQFVRRDPSRIVLL
                     GIEPDSDESEYGYIATGNRLRRGVNGPFHITSFVEKPDYHSAQKLARGGALWNTMIMV
                     FHTGALFELVRTVCPAIFSAMQPIYDAIGSPDEEAVTQEIYQDLLPINFSKEILEPVA
                     HDFPSRLVAFPVRNVLWSDWGSETRVMEILRKIGYASRLNGLAHTADETEPRKDPHPP
                     FAPRGPSVKRHDRAEEELSFASKTQSGTQMLLNEL"
     gene            6706..>7158
                     /locus_tag="VFY96_03370"
     CDS             6706..>7158
                     /locus_tag="VFY96_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5605527.1"
                     /translation="MSAGNLLVVDDDQNLIELIALKLKAEGYEVTTASTGQEAVQAAK
                     AAIFDLCIVDLRLSDQDGISVMRELHSINPGMRVIILTGYGTVESAVQAMQEGAYSYL
                     SKPFNTQELLLQISRALENRRLNSEIQRLKGLLEQAYDFPNIVAKSAKM"
BASE COUNT         1548 a         1897 c         1974 g         1739 t
ORIGIN      
        1 aatgtcacga cgcatccgat cgctgaaccg gccttgtcat ataagggtcc ggcgctcatc
       61 agcatgtgta caatgccgcc gtccctccgg tgcaggttca ctccaacgcc acggatcggc
      121 ttaccggtaa ggcacccttt caggaggtcg gttgtatctt tcaaattcga atcgataagg
      181 cccccgagcg gaaaaacctc gccgaaggat tggccgagca ggcttttgcc ccagaactca
      241 tccgcttccc ggctcgcttg aataatcctc cctgcggaat cgcagaccac aatgatctcc
      301 gccgcttgct cgaggatcga gcttgcgagc cgagcggcag caatcacttc ttcgttgcgt
      361 ttttgttccg ctaggtcggt gaccaccatg cacaaacacg gctcatcgcc gatcgagatc
      421 gcattgacgg agcagtaggt cggcacggac gtgccgtcgc tcgcacaaaa gagtagctcc
      481 gccttcgcca gatactcctg cccacgccgg attagagaat cgtaggccgg gcggtcgact
      541 ggagcgacga actcgcgcgc gagcacaccg ataactttct cgtgtggcaa acgaagcatt
      601 tcggcaaaac gtttattgca gtaaagaatg ctgccgtcgg gaatgagcgt ggctgcgcct
      661 tcgctcattt cctgcagcag catacgcgcg gagtagtcgg cgccgcgcaa agtatagacc
      721 tgatcgcccg cgggactgtt cacaaccaag gcgtcgactt cgccacggcg gatggcatcg
      781 agagtttcct gcgcctcgcg cagttgctct cttaactcct cgacttcggt gagttctctc
      841 gctttgctca tgagaattct ttacgaagct gcggcaagat ccataccgac aagaatgcgc
      901 tcggtgtcgg agagatcgcc gatgaaccgg cgcagcggca gcggcagctg cttcaccagc
      961 gtgggtgcgg cgatgatttg ttcgcccttc gcaagtgcag gcttttggta aatatcgatc
     1021 acctcgagct cgtagcggcc gccgagattg cgctcgcata tatcgcgaat gttggctacc
     1081 gcgcgggtgg agcgcggggt ggtgccggcg atatacagac gcagaatata tttgtcggtg
     1141 ccccgatcgc ggagtggcga ttcgaaactg atcacgcgag agcgcttgtg cggtgacgcc
     1201 atttgactcc ttttaggttc ggctgcgcag atccagtccg atcaagacct tttccgtatt
     1261 cgaaagatcg ccgatgatct tacgcatggg ttccggtaga tttcgcacca gggtcgggat
     1321 cgcgatgatc tgatcgccgc gggcgagttg ggggttttcc agcagatcga tcatctcgat
     1381 ctggtaatga ccgtccaggt attcggcgca gatttttttg agattggcga acgcggcaat
     1441 acacttcggc gtctgtccgg caacgtaaag gcgcaaaagc catttgttgc tttccgtttc
     1501 ggaatttttc gttgccttgg tgaccgacat ggctaagctc cttttccgtc gcgttcccgg
     1561 cgcggtttag taccgccatt acccgaacgt agttgagaca tttgcagtct atccaattga
     1621 agggcttgct cgcgcgtggt ttccagggcg atctcttttt ggagctcttc ggcttcagtt
     1681 tcgaactgcg tgcgtaaggc gacgatctgg gcatcgaggg cgcggcgctt gcgctcgagc
     1741 tcacgctttt tacgctcggt cgcctcgcgg cgaagctgag tggcggcttt ctcgcgggcc
     1801 tcctgtgcgg cgcgggcact gccggtgagc acgccttcgg ttcccacgta aacgtcttta
     1861 agctcgacgc catgatcggt caagataaat tcacggattt gattggaatg agcgataccg
     1921 cgcgccttta ggataaaaag cccgcggttg cgctcgccgt tatgctcgat attgcgcagg
     1981 agaatccagc tatccataag cgatgagacg ccgacttcgc tttgtaactc cgaatggccg
     2041 ccttccacaa gggcgttaaa tatcgaagtg atctgccggc ctttcagata atcgagcaaa
     2101 cgcgtgagca tcgatttcac ttccaagctg tcgccgaggg tcaggtaacc ggataaagga
     2161 tcgaagacgg cgatattcgg cttgatttga tcgatcagtt tgtggatccg caacaggtgc
     2221 atttccaggc cattaaagtt cgggcgggtg gcatcgaaat ggagcaggcc ttttttcatc
     2281 cagggctcga ggtccaagcc gatggatctc agattgcggg tgacctgcgc cgggctttct
     2341 tcgctggaga agtagacaca acgctcgccg cgccggcacg cggcgtccac gaaatgtgcc
     2401 gcgacgctgg tcttgccggt gcccgccgtc cccgagagta gaatgctggt gccgcgataa
     2461 aatcctttgc ctccaagcat ttgatccagg ctatcgatgc cggtggaaac ccgttccgta
     2521 gttgccttgt gctcgaggcc gagggaggtg atgggcagca ccgaaatgcc ttgctcatcg
     2581 atgagaaagg gatactcatt ggtgccgtgg ctcgagccgc gatatttgac cacacgcagt
     2641 cgacgcgtgg agatctgatc gcgcacacga tggtcgagca ggatcacgca gtccgaaacg
     2701 tattcttcca agccgtgccg ggtcaaggtt ccgtcgccct gctcggcggt gatcaccgcc
     2761 gtcacgccct tgtctttgag ccagcggaac agccggcgca attcggcgcg caaaatgttg
     2821 gtattttgaa atcccgagaa aatcgcttcg atggtatcga ggacgacgcg tttggcgccg
     2881 atagaatcaa tcgcatgtcc gagccgaatg aacagtcctt cgaggtcgta ctcgccggtt
     2941 tcctcgattt cgctgcgctc gacccggata tagtcgatca tgagcttctt acgcctgcat
     3001 aactcggcca aatcatagcc aagagacgcg acgttcttgg tcagatcttc ttcggtctcc
     3061 tcgaatgcga cgaatacccc cgggtcatcg tagtcaattg ccccgtggat caggaactcg
     3121 acggagaaaa gcgtcttgcc gcacccggcg cgaccgcaca cgagagtcgg ccgaccgcgc
     3181 ggaatcccac cgaaggtaat ctcgtccagt ccgcgaatgc cggttggcga tttcgccagc
     3241 gccggacgct ccggttcgga ggctttactc attgtttgcg gctgctttct ttctcgtcgg
     3301 acggtcataa ggtggtcggt taaaactgtt tagcgtcttc atacaaacat cattcaatta
     3361 ctgcaagctc tttagaccgg aagatgcctt gcccggccat gtgaaacggt tcagatgaac
     3421 gcttagcgag ttagaattta ctccgtggcg gggtgctttg ggccgtgacg gatgtcacat
     3481 ctgatgggga tattgagtct gatcggcgca ggcttgagaa ctaacccatt gccgcccacg
     3541 ggtcgttaaa attcacggaa aaacagccgc agccctcagt tcgtcaactc gcgcatcacg
     3601 cctttcagct tttgtatgtc gagcggcttg gtgagatacg ccgtggcgcc tttgcgaaag
     3661 cactcgtccc tggtgtctgc cgtcggatac gcggtgatca tgatgacccg cgttgacggt
     3721 gcgcggcgtt tgatctcgga caaaagcgcg acgccatcga gcgggaacat cttgatgtcc
     3781 agaatggcgg cgcgaaagtc atttttaccg atcagatcga gagcctggtt gctgccatgg
     3841 gcgacagtca cggcgtagcc ttctttactg aggatccggt tcagcagtac cagcagattc
     3901 tcttcatcat cgacgatcag gatcgagttc ggcattatcg atgcacagac attatccatc
     3961 gtgatccgag ctatgatgcc gtaggggaac agcctcggca ccatgtgtta tttgtttatg
     4021 cttctctgtg ttccgtcttt aagcgggtcg cgcggagccg gatcaccggt gtagcgccct
     4081 ttagcgcgtt cacgcttcta ccactgtggt tttttcctgg caatatcttt cgcttgcaag
     4141 gttgaaagaa attccgaggt ctcggtccgc aagaccacgg ccgagatccc gtgatcatag
     4201 gctcactttt ttccctggat ctgcaaattg tttttgaccc ctttgacccc tgaagtctgc
     4261 cgcgcaagct gctcagcgcg ttgcttttgc tccgctgact cgacaatgcc gttgagcgaa
     4321 acaatctgat tggtggtgtc gacgtcaacg cgggtcaggt ttgcgacctt gtccgctact
     4381 aagttcgatt tcacgcgtgc cgtgagcgta gagtcgtcga catattgccc ggcactctcg
     4441 ccggtcagtg ccgtgcatcc gctggtggct agaaaaattc ccaggagcaa tgatgcgccc
     4501 gatttccacc atcgcggaca atctcttttt aaaaacatat caacctcctc gaggatcttt
     4561 tactaatgtc gtctctacag tcgtccagtg agtaaaagga tgacgatgat cagtacgatc
     4621 aaacccagcc cgccgctcgg atagtaaccc cagcccgagc tgtagggcca agtcggcagc
     4681 gcgccgatta acagcagaat gaggataatc agcaatatgg tacccatacg attgcctccg
     4741 ctgtcctgag ttagagctca ttgagtaaca tttgagttcc cgactgagtt ttcgacgcga
     4801 aagaaagttc ctcttcggca cggtcgtgtc gctttacgct aggtccccgc ggtgcgaagg
     4861 gcggatgggg atctttgcgt ggctcggttt catccgcggt gtgggccaag ccattgaggc
     4921 gcgatgcata gccgatcttt ctgagaatct ccattacacg cgtttccgag ccccaatcgc
     4981 tccataagac attgcgcacc gggaatgcga ccagccgaga cggaaagtca tgcgccaccg
     5041 gctccaatat ctctttagag aaatttatcg gcagcaagtc ttggtaaatt tcctgcgtca
     5101 ccgcctcttc atccggactt ccgatggcat cgtaaatcgg ttgcatcgcg ctgaaaatgg
     5161 ccggacaaac ggtacggacc aactcaaaga gcgcgcccgt atgaaacacc atgatcatgg
     5221 tgttccacag ggcgccgccg cgggcaagct tttgtgctga gtgataatcg ggtttttcca
     5281 caaacgaagt aatatgaaac ggaccattga cgccgcgccg taaccggttg ccggtcgcga
     5341 tgtaaccata ttcagattca tcgctgtccg gctcgatgcc gaggagtaca atgcgcgatg
     5401 gatcgcgcct gacgaactga ccggcgtatg tgaggtagcg catcatctct tcttcttgca
     5461 aaataaaatg gtcggaaggc aaaagtgtca ccagcgaatt tgggcaacgc ttggcaatgt
     5521 gcaaaagagc gaagatgatc cctgtggcgg tttctttatt ttccggctga accagcacgg
     5581 tgcccgggct tcgctgggct atttgtcgtt gcacatccgg gaaagccaaa tgactgcgat
     5641 tcactactgt gaaaatgctg gacggcgcca tgagcttttc ggctcgatcc caggtgtgct
     5701 cgagcatgga acggcggccg ataaaattga catattgctt gggtaagaaa tctccacgca
     5761 ggagatgaat gaaggacttt agtctcttac cttctccacc agccagaaca agtgcgtatg
     5821 aagtaggcga tttctttatg gccggctttt tggagttcaa aacattttcc aacatgcttg
     5881 cttgtgtcga tccggcgact aggccgcccg cttcaccgca atccgatcat tgatcgtcaa
     5941 cccggcgtgg gccgacttcg ctaattcgcc gctctgtggc gaaaccgcct cagaggagac
     6001 gagtccagaa agagtcgctt cgtttttcgt ggcttctgca tgacgtcgat ggcggcctcc
     6061 agagcagcat ctgtttcgag tttggttcgg atgcgtcgct ccaattccag gttatgaatt
     6121 acaaagtcct cgaatcgggc tcgacaggtt gtatgttcgc aggatgtccg tcacccaata
     6181 cgaaccaggt taacatcaga atcgccagta ccaacgaata caggctgatg cgggtgatgg
     6241 ttccgggcga ttcgggctgg gatagccgaa acacaaagaa tgtgccaaaa cgagaatgct
     6301 ttatcttatt gaatttaaac atgaattatg tgaagcttaa cgtgatttgt aagtttaacc
     6361 acacataacg gcagaaaact ctgacaccga gaagagcttt tttgtgtgtt cggttcgtac
     6421 aacgaatgat ggttacgatg atggttactt gtaatttgtg gcgcaggcat tgcgcttaag
     6481 actccgctga agcaattttg gttagaatat tgcagtgtcg ggaataacgg tccaagaacg
     6541 gccgtagttt gttctggtgt ttgggattta cctacttttc tgtatggtaa atacttacta
     6601 gtttttattc gcctggatta attgttcttg tcaagcatcg tgcgggcgca gcaatcatcg
     6661 ctgcggagat gctttttgta aattttacat atagagagaa tagccatgtc agcgggtaat
     6721 cttcttgtcg ttgatgacga tcaaaatttg attgagttga tcgccctgaa gttgaaagcg
     6781 gaaggatacg aggttacgac cgcgtcaacc ggtcaggagg cagtgcaggc cgccaaggcg
     6841 gctattttcg atctttgtat tgtcgatttg cgattgtctg accaggatgg catctccgtg
     6901 atgcgcgagc tgcattccat caatcccggc atgcgtgtca ttattctaac cggttatgga
     6961 accgttgaga gcgctgttca ggcgatgcag gaaggcgctt acagttatct cagtaaaccg
     7021 ttcaatacgc aggagctgtt actgcagatc tcgcgcgcat tggaaaatcg ccggctcaat
     7081 tcagaaatcc aacgcttaaa aggattgctg gagcaagcat acgatttccc caatatcgtt
     7141 gccaaaagcg ccaaaatg
//
LOCUS       DASULZ010000164         3044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1440067, whole genome shotgun sequence.
ACCESSION   DASULZ010000164 DASULZ010000000
VERSION     DASULZ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440067"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..2512
                     /locus_tag="VFY96_03375"
     CDS             182..2512
                     /locus_tag="VFY96_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5605528.1"
                     /translation="MPTYCAMCTSRCGVIATIENGRLIAVNADPQHPNGCICVKGAAA
                     PDIVYSPDRVQYPLLRSRPKGDPDAGWLRVTWDQALNLTALRLNDVKARYGAEAVVFS
                     RATTAGSAAIDYDGWLQRLANAFGSPNFLTSNHICTWNRRVGSKYTYGVGMPLPDFDH
                     TRCILLWGINPTATSPAQAVRVNRALNRGAKLIVVDPRKTTLAGRADYWLRVCPGSDG
                     ELAMAMIDVLLTEKLFDEEFARRWTNAPYLVRNDTGRLLSEADLREAGQVDTWLVWDE
                     QRKFPVNCAAAVVSPALFGSFAVRLLNGTTLQCRPALQLLKEAAAEFTPEISEKITTV
                     PADEVRKAVRLFATEKPSCYCTWVGLEQDRDAMQTNRAVCLFYGLTGQFDQRGSNVLF
                     ATTPTNPINGRELLPKEKDVTRLGLAEHPLGPPRDTGIVQAGRVYDAVLTEKPYPVKA
                     LVAFGSDALLGHGDPLRGKAALESLQFYVHVDTTINPSATFADLILPATTCWEREALL
                     PSSEIAEDTLNWAQLRPAVVNPVYESRSDTEIVFELAKRLELSDNFFGGDLDAGFDYQ
                     LNPSGLSSQRLRAHPRGIRADVQTRHRKYAEIDAARGQARGFETPSGKVEIFSSIFAE
                     TGYAPLPDFPSDTFSADHLAYPLILTFFRDIHFCDEQHRNILRLRRALPDPFLEIHPS
                     TALAREIQNGDRISLETAGGTVQLKARFNESLHPTVVATVYGWWQACKELNLPGHNPF
                     SENGANLNLLVPNADADPISASVAHRGQRCRVIKIS"
     gene            2689..>3044
                     /locus_tag="VFY96_03380"
     CDS             2689..>3044
                     /locus_tag="VFY96_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX5605529.1"
                     /translation="MDIFWEELTSGLPDTRQLVHVLIKLIAASLLGAVVGLQREKAHK
                     PAGLRTHILVSLGTAVLVLACSGVGMSLDGISRVIQGIATGVGFVGAGSILKLNEERH
                     IEGLTTAAGLWMTAAIG"
BASE COUNT          657 a          883 c          847 g          657 t
ORIGIN      
        1 aaagcatatg ggaatgacga tctcagggtg attaccgatt cagcttcaaa cgtcgagttg
       61 tcctcccgaa cgaatgtgag ggatctttcc ggaagtattc ctcgcgcgcc gttcctcgga
      121 atgagacgat ttgtagctcc cttgagtttc aacggagaat tttaaaatcg ctactgaaag
      181 gattccaacc tactgcgcga tgtgcacatc gcgctgcggc gtgattgcaa ccatcgaaaa
      241 cggcagactc atcgccgtca atgccgatcc ccaacaccct aacggctgca tctgcgtaaa
      301 aggcgccgcc gcgccggata tcgtctactc gccggatcgc gtgcaatatc cgctgctgcg
      361 ctcacggccg aaaggtgatc ccgatgctgg ctggctgcgt gtgacttggg atcaagcctt
      421 gaatctcacg gctctgcgcc tgaacgatgt caaagcgcgc tacggcgctg aagccgtggt
      481 gttttcccgc gcgacaaccg cggggagcgc tgccattgat tatgacggct ggttgcagcg
      541 cctggcgaac gctttcggga gcccaaattt tctcacgtcc aatcacatct gcacgtggaa
      601 tcgccgcgtc ggctcgaaat acacctacgg ggtcggcatg ccgctgccgg atttcgatca
      661 tacacgctgt attttgctgt ggggaatcaa tccgacggcg acatcgcccg cgcaggcagt
      721 gcgcgtgaat cgagctctca atcgcggcgc taaactgatc gtcgtcgatc cgcgcaaaac
      781 aactctggcc gggagggcgg attattggct acgggtctgc cctggtagcg acggcgagct
      841 cgccatggcg atgatcgacg tgcttctcac cgaaaaactc ttcgacgaag aattcgcgcg
      901 cagatggacc aatgctccct atctggtccg caacgacacg ggtcgattgc tttctgaagc
      961 tgatctccgc gaggccggtc aggtagatac atggttggtt tgggacgaac aaaggaagtt
     1021 tcctgtcaat tgcgctgctg ccgtcgtttc gcccgcgctt ttcggctcat ttgccgttcg
     1081 acttttaaac ggcacgacgc ttcagtgtcg gccggcgctt caattgctca aagaagccgc
     1141 cgccgagttc acaccggaga tatcggagaa gattaccacc gtcccggccg acgaggtccg
     1201 caaagctgtg cggctgttcg cgaccgaaaa accttcctgc tactgcacct gggtcgggct
     1261 cgaacaagac cgcgatgcga tgcaaactaa ccgcgcggtg tgcctcttct atgggttgac
     1321 tgggcagttc gatcagcgcg gcagtaacgt gctgttcgcg accactccga cgaatccgat
     1381 aaatggtcgt gaattgctgc cgaaagaaaa ggatgtgacg aggctcggac tcgccgagca
     1441 ccctttgggt cctccgcgtg atacgggcat cgttcaagcg ggccgcgttt acgacgccgt
     1501 tctcacggaa aaaccgtatc cggtgaaggc gctggtcgcg ttcggtagcg acgccctact
     1561 tggccatggc gatccgctgc gtgggaaagc ggccctcgag agcctccaat tttacgtcca
     1621 tgtcgatacg acaattaacc caagcgcgac gtttgccgat ttgattcttc ccgctacaac
     1681 ctgttgggag cgcgaagcac tgttgccgtc atctgagatc gccgaggata cgctgaattg
     1741 ggcacaactc cgtccggcag ttgtcaatcc cgtgtacgag tcacgctcag acacagaaat
     1801 tgttttcgaa ctcgccaagc gactggagct ctctgacaat tttttcggcg gtgatctcga
     1861 cgccggcttc gactatcaat tgaacccttc gggccttagc agccaacgat tgcgcgcgca
     1921 tccgcgtgga atccgagctg acgttcaaac tcgccatcga aagtatgccg agatcgatgc
     1981 cgccaggggt caagcgcgcg gattcgaaac cccgagcggg aaagtcgaga tcttctcctc
     2041 aatttttgcg gagaccggat atgctcccct acccgatttt ccgagcgaca cattcagcgc
     2101 cgatcacctc gcttatccgc tcatccttac tttcttccgc gatattcact tctgcgatga
     2161 gcaacatcga aatattctcc ggctgcgccg cgcgctgccc gatccgttcc tcgagattca
     2221 tccgagcacc gcactggcgc gcgaaatcca aaacggcgat cggatctcac tcgagacagc
     2281 gggtggaaca gtgcaactga aagccagatt caacgaatcc cttcatccga cggttgtcgc
     2341 aaccgtctac ggctggtggc aagcctgcaa agaactgaac cttcccggtc acaacccgtt
     2401 cagtgaaaat ggcgcaaatt taaatttgct cgttccaaat gccgacgccg acccaatcag
     2461 cgcttcggtc gcccatcgcg gtcagcgctg ccgagtgata aaaattagct gaactccttg
     2521 cagacgtcgt attgacatac gaactgcgga tcgattttct gtgtaagggc gaagcacgct
     2581 gcgcccctgg cattgcgctg caatagtgtt caaccggcaa attccggcgc catgaagtta
     2641 gagacgctga aatgggctag tcatctacga agttaatggg gatctaagat ggacatcttc
     2701 tgggaggaac taacgtccgg actacctgac acgaggcagt tagtacatgt actcatcaag
     2761 ctcattgccg ccagcctgct cggcgctgtc gtcggcttgc aaagagagaa ggcgcataag
     2821 ccggcagggc tcagaacgca tatactggtc agcctcggca cagccgtctt ggtcctggct
     2881 tgctccggag tgggcatgtc cctcgatggg atatcgcgag taattcaagg catcgctacc
     2941 ggcgtcggct ttgtgggtgc gggctccatt ctcaagctca acgaggagcg gcacatagag
     3001 gggctaacaa ccgcggctgg gctgtggatg acggcagcga tcgg
//
LOCUS       DASULZ010000165         2747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1339280, whole genome shotgun sequence.
ACCESSION   DASULZ010000165 DASULZ010000000
VERSION     DASULZ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339280"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VFY96_03385"
     CDS             complement(<1..400)
                     /locus_tag="VFY96_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HEX5605530.1"
                     /translation="MTDSTASGNFIELTAEIVSAYVSNNTVPAGEIPGLINQVHAALA
                     RVSGKPAEGSSEPLRPAVSVKKSITPEYIVCLEDGKKFKSLKRHLRTQYNMTPEQYRE
                     KWGLNADYPMVAPNYAAARSQLAKQMGLGQQ"
     gene            complement(642..1532)
                     /locus_tag="VFY96_03390"
     CDS             complement(642..1532)
                     /locus_tag="VFY96_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605531.1"
                     /translation="MAVLLFSIGTFALVAGLVMVGFGIPINEFSFGNTLISAGTTAAV
                     GGLIIVALGVAVGQLRRIAEALAGDLPARYPQPMDLVEATGTPSPAAGIPDRIPFPSR
                     PKSETPFPKMGSLPGAPTNTASDDDYDRFAAPTLPNPEEAPMAVEDEISLSPPQPSGG
                     SAPTADVRGPAGRGQEPPTSWKPSPASTPPVGSPPRPLQPAKFDAMWPAEAKSPRPGV
                     SEDAKPPSAPARETFPPVPKRPEPEAQKPRAVAILKSGVVDGMGYTLYVDGSIEAELP
                     QGTLRFASINELRSHLEKNS"
     gene            1827..2429
                     /locus_tag="VFY96_03395"
     CDS             1827..2429
                     /locus_tag="VFY96_03395"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX5605532.1"
                     /translation="MSFKLPDLPYAHDALAPYMSRETLEYHHDKHHKAYVDNGNKLLE
                     GSGLENKSLEDVVKGSFGKNAGLFNNAGQHYNHTHFWKWMKPNGGGDKIPAKLKTKID
                     SDLGGVAKMKEDFTQAGVTQFGSGWAWLAVKDGKLIVSKTPNGENPLVHGATPILGCD
                     VWEHSYYIDYRNRRPDYLKAFLDHLVNWEHVEEMFVAATK"
     gene            2458..>2747
                     /locus_tag="VFY96_03400"
     CDS             2458..>2747
                     /locus_tag="VFY96_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605533.1"
                     /translation="MFRTHPADGRLFADRLALVVLGVVAVIAAATFRDYGLGWDDYTH
                     SEYGDLLLAFYGSGFSDQRALSFVNLYKYGGGFDMAAAFAAKVLPFGLFETRR"
BASE COUNT          593 a          781 c          778 g          595 t
ORIGIN      
        1 gctgttgccc aagccccatt tgcttcgcga gctgcgagcg tgctgcggca taatttggcg
       61 cgaccatcgg gtagtcggcg ttcaaacccc atttctcacg atactgctcg ggcgtcatgt
      121 tgtattgggt acgcaaatgc cgtttgagcg atttgaattt tttgccgtct tcgagacaga
      181 caatgtactc gggggtaatt gatttcttga ccgaaacagc tggcctaagc ggttcggagg
      241 agccttccgc aggttttccg gagactcttg cgagcgcggc atgaacctga ttgatcagcc
      301 caggaatctc accagcgggc actgtattgt tgctgacata agcggacacg atttctgcgg
      361 tcagctcgat gaaatttcca ctggctgtgc tgtctgtcat tgcaactgcc ccgtttgacg
      421 aggctgccgt tgtcggcaac ccttatcccc ttcaaacagg ccctctcagc caacagccgc
      481 acgccgacga atggaggacc tatatttaaa actactatcc cacttctgta gttgcaagaa
      541 aaagctcatt gtctgatacg acaatattgg gtcgtatgcg acgaaagcag aatctcggga
      601 aagaaaaacc aagaaaagta gaagcaggcg cagtccggct cttatgaatt cttttcgagg
      661 tgacttcgga gttcgttgat cgaggcgaat cgcaatgtgc cttgtggtaa ttccgcctcg
      721 atcgaaccgt cgacataaag ggtgtagccc atcccgtcga cgacgcctga tttcagaatg
      781 gcgacggcac gtggcttttg cgcttcgggc tctggacgtt tgggtacggg aggaaacgtt
      841 tcgcgtgccg gcgccgacgg tggcttggca tcctccgaga cgcccggcct tggtgacttt
      901 gcttcagccg gccacatcgc atcgaatttg gctggctgaa gcggccgcgg aggcgatcca
      961 accggcggag ttgacgcagg ggatggtttc cagcttgtgg gcggttcttg accccggcca
     1021 gcagggcccc tcacatcggc cgtcggggcc gaaccgccag atggctgagg tggcgacagg
     1081 gaaatttcat cctccaccgc catcggtgct tcttccggat tgggcaaagt tggcgccgca
     1141 aaccgatcgt aatcgtcatc gctcgccgta ttcgtcggtg cacccggcaa cgatcccatt
     1201 ttcgggaacg gtgtctccga tttagggcgc gacgggaagg gaatccgatc aggtatgccg
     1261 gccgcaggtg agggagtacc cgtggcttcc acaagatcca tcggttgcgg gtacctcgcc
     1321 ggcaaatcgc ctgcgagcgc ctcggcaatc cgccgcaact ggccgaccgc tacgccaagc
     1381 gcaactatga tgaggccgcc gacggccgcc gtggtgccgg cgctgataag tgtatttccg
     1441 aagctaaatt cgttgatcgg aatgccaaat ccaaccatca ccagccccgc cacaagggcg
     1501 aaagtgccga tgctgaacag caataccgcc atactttatg atcccctctc gatgatcccc
     1561 tctcgaccag caactttcgc cgcggctatc cggctaaatt cttctggcaa aggcaaattc
     1621 tcgctttttc gaaagccggc ggcaaccact aaatagaccg tgatttcttc gctaaaagtc
     1681 atctaatgcc gggcccggct acgctcggtt gctcggagcc gcatcaccgc ctaattagct
     1741 tgactgagga actcgtccgg gacaggccag tttcatccga gaaccagttt attccgattc
     1801 atcgtttgtg caccgaggag gatctgatgt cgttcaagtt gcccgacctg ccgtacgctc
     1861 acgatgcgct cgcgccctac atgtcgcgcg agacactcga ataccatcac gacaagcatc
     1921 acaaggccta tgtcgacaat ggcaacaagc ttctggaagg cagcgggctg gaaaacaaat
     1981 cgctcgagga cgtagtgaaa ggatcattcg gcaagaacgc ggggctcttc aacaacgccg
     2041 gccagcacta caaccacacg catttctgga aatggatgaa gccgaatggc ggcggtgaca
     2101 agattccagc aaaacttaag acaaaaatcg acagcgatct cggcggcgtc gccaagatga
     2161 aggaagattt cacgcaggct ggcgtgacgc agtttggctc aggctgggcg tggctcgcgg
     2221 tcaaggatgg gaaactgatc gtcagcaaga caccgaatgg cgaaaacccg ctcgtccatg
     2281 gtgcaacgcc gattctcggc tgcgacgtgt gggagcactc ctattacatc gactaccgca
     2341 accggcggcc cgattatctc aaggcgttcc tggatcactt ggtgaactgg gagcacgtcg
     2401 aagaaatgtt cgttgcggca accaagtagc caaacgcagt ggaccgtacc tcgcgggatc
     2461 ttccggacac atcctgccga cggtcggctg ttcgccgacc ggctggctct tgtcgtcctt
     2521 ggggtcgttg cagtcatcgc ggcagcgacg ttccgtgact acggtctggg gtgggatgat
     2581 tacacccatt ccgaatatgg cgatctgctg ctcgccttct acggctcggg tttttcagac
     2641 cagcgcgcgc tttcattcgt caacctgtac aaatacggcg gcggcttcga tatggctgcg
     2701 gcattcgcag cgaaggtact gccattcggt ttgttcgaaa cacggcg
//
LOCUS       DASULZ010000166         3545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_222185, whole genome shotgun sequence.
ACCESSION   DASULZ010000166 DASULZ010000000
VERSION     DASULZ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222185"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VFY96_03405"
     CDS             <1..711
                     /locus_tag="VFY96_03405"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5605534.1"
                     /translation="EAMIAKMDEAGVEKVFITQCKMWSYRNKWMYMDTELEEVAQYTK
                     RYPKRFVGLAGYNPFRIKESLEEIDRAVKHYDFKGVYIHIYGFDIPLHDAKMYPLYAK
                     CDELKIPVSMQVGHVLEAMPSEHARPVYLDRIASDFPDLKIIGAHTGWPWVEELISVC
                     YKWDNVYFGVDAWMPKYLKPEIIHYINSRMGQDRCLWGTNGLPWKESLDQWEAVGLKS
                     DTKRHLLHDNAVQLFGLQ"
     gene            721..1500
                     /locus_tag="VFY96_03410"
     CDS             721..1500
                     /locus_tag="VFY96_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX5605535.1"
                     /translation="MYQTLQYHENDAIATIQLHRPQKKNSLNAEMRQEMERLFRDIMR
                     NTAIRAVIITGNDEIFCAGADISEMEGTASAEAAYQHAREFQILFDQIESLPQPIIAA
                     VSGYALGGGCELALAADFRIASESARFGLPEIKIGAFPGGGGTQRLARLIGVAKAKEM
                     IFTGDPITAQEALAVGLVTKVTARDSYLAEATLFADKLAALPRLALQASKMLINRGRD
                     VDLATGLEFEARTFASIAHSHDLAEGTKAFLEKRKPHFTGY"
     gene            1560..2354
                     /locus_tag="VFY96_03415"
     CDS             1560..2354
                     /locus_tag="VFY96_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017616758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HEX5605536.1"
                     /translation="MNYNSFVFENNDGVATIRLNDPEKLNALTFQVYGDLEKIFAELA
                     DDHQTKVVVLTGTGKGFCSGGSVNDIIGPLLKMKGDELYKFTRMTCNVVKNMRQLKKP
                     IIAAVNGIAAGAGAMLMLASDLRIFSDKARAAFLFVKVGLSGADMGALYLLPRIVGLG
                     KATELVYFGDTVDATEAYRIGLANRVVPGEALMDEAYKWANRLKDGPLYALGVTKELL
                     EYEANVNLETALEMEAAAQARCMETPDFIEGYTAFVEKRPLKFNRG"
     gene            2377..2826
                     /locus_tag="VFY96_03420"
     CDS             2377..2826
                     /locus_tag="VFY96_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5605537.1"
                     /translation="MKKEIYVRNLEKRDLSAIVSMEERQTGMARPDYWDKRIEISEAI
                     RPHWASLVAEVDNRVVGFVLGRAGEYEFGLPGTIAWIEIIGVDPAYRHHGIAQELVRQ
                     FAESAEDHGIKTIFTLVASDRPEMQQFFTQVGFLHGKMLHYQKELST"
     gene            2834..>3545
                     /locus_tag="VFY96_03425"
     CDS             2834..>3545
                     /locus_tag="VFY96_03425"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5605538.1"
                     /translation="MDSFTDPDHELRGSLRSWAERNPLVGETDQEALEREACSLARRL
                     GREGFMAYAVPRLYGGMREAVQARDLCVIREELAYFAALADVMFGVQALASQPIVITG
                     TEEQKKKYLPPLARGETLGAFALTEPEAGSDASAIALRAKRVIGGYSLTGTKRFISNA
                     GIAQTYIVFASTDPSAKAKGISAFIVEADSPGFTIKEKTRLLSPHPIGVLGFDDCFVL
                     QERLLATEGSGLKIALQTL"
BASE COUNT          868 a          924 c          996 g          757 t
ORIGIN      
        1 gaagcgatga tcgcgaaaat ggacgaagcc ggtgtcgaga aagtcttcat aacgcaatgt
       61 aagatgtggt cgtaccgcaa caagtggatg tatatggata ccgagctgga ggaagtggct
      121 cagtatacca aacgttatcc aaaacgattt gtcggtctcg ccggctacaa tccatttcgc
      181 atcaaggaat cactggagga aatcgatcgc gcggttaagc actatgactt caagggcgtc
      241 tacattcaca tctacggttt cgacattccg ttgcacgatg ccaaaatgta tccgctctat
      301 gccaagtgcg atgaactcaa gatccccgtt tccatgcaag tcggccacgt gctggaagcg
      361 atgccgagtg aacatgcccg gccggtttat ctcgatcgta tcgccagtga ctttccggat
      421 ctgaaaatta tcggtgccca taccgggtgg ccctgggtcg aggagctgat ctcggtgtgc
      481 tacaagtggg acaatgtcta ttttggcgtc gatgcctgga tgccgaaata tttgaagcct
      541 gaaatcattc actacatcaa tagccgcatg ggccaagacc gctgtctatg gggaaccaac
      601 ggcttgccct ggaaggaaag cctcgatcag tgggaagcag tcggcctgaa atcagatacg
      661 aagcgacatc tcttgcacga caatgccgtg caactgtttg gccttcagtg agagtacgat
      721 atgtatcaaa ccctgcagta ccacgagaat gacgcaatcg ccacaattca actccatcga
      781 ccgcagaaga aaaattcgct gaatgctgag atgcggcaag aaatggagcg actgttccgc
      841 gacattatgc gtaatacggc cattcgggcg gtcattatca cagggaatga cgaaatattc
      901 tgtgccggcg cagacatcag cgaaatggaa ggaacggcca gcgctgaggc ggcctaccaa
      961 catgcgcggg agttccagat tctcttcgac cagatagaat ccttgcccca gccaatcatc
     1021 gccgcagttt ccggctatgc gctcggcggc ggctgtgaat tggccttggc ggctgatttc
     1081 cgcatcgctt cggagtccgc gcgctttggc ttgccggaaa tcaagatcgg cgcattcccc
     1141 ggcggcggcg ggacgcagag actagcgcgt ctgatcgggg ttgccaaagc gaaagaaatg
     1201 atcttcacgg gagatccgat tacggcgcaa gaggcactcg cagtgggttt ggtgacgaaa
     1261 gttaccgcaa gagacagcta tttggcggag gcgactctct tcgccgataa acttgccgca
     1321 ctgcctcgtc tcgcattaca agcctcgaaa atgttgatca atcgcggtcg cgacgtggat
     1381 ctagctacgg gcttggaatt cgaagcgcgt accttcgcta gcatcgccca cagccatgat
     1441 cttgccgaag gaactaaggc gttcttggaa aaacgcaaac cgcattttac cggctattga
     1501 ttacgggatt acagcgagta tcgtcgcaaa aaagaagcta gtgaggagaa ccagacggaa
     1561 tgaactacaa cagttttgtt ttcgaaaaca acgacggcgt cgcgacgatt cgactcaacg
     1621 atccggaaaa gctcaacgcg ctcacttttc aggtttacgg cgatctggaa aagatctttg
     1681 ctgaactcgc ggacgaccac caaacgaaag tcgttgtcct gaccggcacc ggaaagggtt
     1741 tctgctccgg cggcagcgtc aatgacatca tcggtccgct gctcaaaatg aagggagacg
     1801 agctctacaa gttcacgcgc atgacctgca acgtcgtaaa gaacatgcgc cagctcaaaa
     1861 aaccgatcat cgccgccgtc aatggcatcg ctgccggtgc cggcgccatg ctgatgctcg
     1921 cttcggatct gcgaatattc tccgacaaag ccagagcggc gtttctgttt gtaaaggtcg
     1981 ggttgtccgg agcggatatg ggcgcccttt atttgctgcc tagaatcgtc ggtttaggca
     2041 aagccaccga gctggtttac ttcggcgaca ccgtcgatgc gaccgaggcc taccgtatcg
     2101 gcttggccaa tcgggtcgtg cccggcgaag ctttgatgga cgaagcttat aaatgggcaa
     2161 accgcctgaa ggacggccca ctttacgctc tcggtgtgac caaagagttg ctcgaatatg
     2221 aagcgaacgt aaatttggaa accgcactag aaatggaagc ggcggcccag gcgcgctgca
     2281 tggagacgcc ggactttatc gaagggtaca ccgccttcgt cgaaaagcgg ccgttgaagt
     2341 tcaaccgagg gtaatcccgg gctgtggaga agaaatatga aaaaagagat ttacgtaaga
     2401 aacctggaga aacgcgatct gtctgccatc gtcagtatgg aggagagaca aaccggtatg
     2461 gcacggcccg attactggga caagagaatt gaaatttcgg aggccattcg cccgcactgg
     2521 gcatcgctgg ttgccgaagt tgataatcgc gtcgtaggat tcgtattggg gcgcgccggt
     2581 gagtatgaat ttggtctacc gggcactatt gcatggatcg aaattatcgg agtcgatccg
     2641 gcatatcgcc atcacggaat cgctcaggag ctcgtcagac aatttgccga atcggctgaa
     2701 gatcacggca tcaagaccat cttcactctc gtggcgagtg atcggccgga aatgcagcag
     2761 ttctttactc aggtaggctt ccttcacggc aagatgctcc actaccaaaa agagttgtct
     2821 acctgacccc ccgatggact ccttcaccga ccccgaccac gaactgcgtg gaagcctgcg
     2881 ctcatgggcc gagcgaaatc cgctcgttgg tgagacggac caagaagcgc tcgagcgcga
     2941 agcgtgctca ctcgcgcggc gactcggtcg ggagggtttc atggcctatg cggttccgcg
     3001 tttatacggc gggatgcgcg aagccgtcca ggcgcgggat ctctgtgtga ttcgtgaaga
     3061 gctcgcgtac ttcgcggctc tcgccgacgt catgttcggc gtgcaagcgc tcgccagtca
     3121 accgatcgtc attaccggca cggaagaaca aaaaaagaaa tacttaccgc cgttggctcg
     3181 aggggaaacg ctcggagctt ttgcgttaac cgagcctgaa gctggctctg atgcctcggc
     3241 tattgctctg cgggcaaaaa gagtcatcgg tggttactct ttaacaggga cgaaacgttt
     3301 catttccaac gctggcattg cgcaaaccta cattgttttc gcgtccacgg accctagcgc
     3361 caaggcgaag ggaatcagcg ctttcatcgt cgaagcggat tcgcccgggt tcaccataaa
     3421 agagaaaact cggctgttat ctcctcaccc catcggtgta ctcggttttg acgattgttt
     3481 cgtgctgcag gagcgattgc tcgcgacaga aggaagcggg ttgaaaattg cactgcagac
     3541 tctgg
//
LOCUS       DASULZ010000167         2743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1339353, whole genome shotgun sequence.
ACCESSION   DASULZ010000167 DASULZ010000000
VERSION     DASULZ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339353"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VFY96_03430"
     CDS             <1..210
                     /locus_tag="VFY96_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605539.1"
                     /translation="LLNEDIMPERATNTEQLDEHLLEVVRALHGAVRQGVSHTDDAKI
                     TDQAIEDCKEILDVIMAGKVNEWLN"
     gene            260..754
                     /locus_tag="VFY96_03435"
     CDS             260..754
                     /locus_tag="VFY96_03435"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX5605540.1"
                     /translation="MSRAAEYFYQTEIEWTGEKDMKLSSGNLPTITAGAPPEFKGRGE
                     NWSPEHLFVASLNSCYALTLLAIAELSKITIVSLSCSAKGKLEKVEGQSYQITEIIVK
                     PRLVIASVNDLSRMPRIMEKAKENCFVSNSIKSTVKIEPEIFHQQTPASPCALGQAPA
                     GIDD"
     gene            1052..1202
                     /gene="frdB"
                     /locus_tag="VFY96_03440"
                     /pseudo
     CDS             1052..1202
                     /gene="frdB"
                     /locus_tag="VFY96_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787978.1"
                     /note="part of three member fumarate reductase enzyme
                     complex FrdABC which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdC is the
                     cytochrome b-556 subunit; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur subunit"
     gene            1389..>2743
                     /locus_tag="VFY96_03445"
     CDS             1389..>2743
                     /locus_tag="VFY96_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEX5605541.1"
                     /translation="MSKTMAKPGLIVSGGVAHAEKETKPLGNYWKALIPIAAGALLLL
                     LPIPEGLKPYAWYYFALFVAVIIALILEPIPAAAVGLIGVTAATITPWVAAKPADSIK
                     WALSGFQDGTVWLIFVAFMFALGYEKTGLGRRVALNLVKWLGKRTLGLGYAVALADLV
                     LAPFTPSNTARSGGTIFPVIKNIPPLYGSEPGPTARKIGSYLMWTAFATTCLTSAMFL
                     TGLAPNLLALSLVKTTAKIDISWTEWFIGIMPVGVLLFIFLPLIVYKIYPPEVKSSPD
                     VPVWAGSELKKMGRISAKEVGMALLALVALGLWIFGGDLINATTVAILVLCLMVICRI
                     VEWDDVLANKQAWNVLVWFATLVTMADGLNRVGFLKWFATGAAGALGGYSVMTIVIVL
                     TVVYFVVHYMFASLTAHTTALLPVLLTAAIAVPEMPVKLISLLFCYTLGLMGIISPYA
                     TG"
BASE COUNT          611 a          704 c          779 g          649 t
ORIGIN      
        1 ttgctaaacg aggatattat gccggagcga gcaaccaaca ccgaacagct cgatgagcac
       61 cttctcgaag ttgtccgcgc tcttcacggc gcggttagac agggcgtgtc tcacaccgat
      121 gatgcgaaaa taaccgatca agccatagag gactgcaaag aaatccttga cgtgatcatg
      181 gccggaaaag tgaacgagtg gttgaactga attggatcag ctgacgacga tccgataatc
      241 ggatccagag gaacattata tgagtcgggc ggccgagtat ttttaccaga ccgagatcga
      301 gtggacgggt gagaaagata tgaagctcag cagcggcaac ctgccgacga tcactgccgg
      361 cgcgccgcct gaattcaaag gccgaggaga aaactggtcg cctgagcatt tattcgtggc
      421 ctcgctcaat tcgtgttatg cgctgacgtt gctcgccatc gcggagttgt cgaagatcac
      481 gattgtgagt ctctcgtgca gcgccaaggg taagctcgaa aaagttgaag gacaaagcta
      541 tcaaatcacc gagatcatcg tgaagccgcg gcttgtgatc gcctccgtca atgacctttc
      601 gcgcatgccg cgcatcatgg aaaaagcaaa agagaactgc ttcgtttcga attcgatcaa
      661 aagcaccgtc aaaatagagc ccgaaatctt tcaccaacag acacctgcct caccttgtgc
      721 attgggtcag gcgccggccg gtatagacga ttagtaggct caactgggtt gagcacaatt
      781 ccttccgttg cacatcgctt tcgcattttt gacgattctc actcaaattt ctatcagagc
      841 tgacaagtct tggggtcgcc ggggccttca actgccgata attatacttg gtagtgcgtt
      901 gctctgctga gtttaatcag tgggacgcta cagtggttaa atcaagtaaa tgaccataag
      961 caaggctcta atgtcatgac cggaactatc gcaagctcgc cgccgatgac cagaacatct
     1021 gtgtagatga tcttcataat gcaggcgtcc gatcagcgac gacggcggtt ttttcggctg
     1081 catggcactg cttggctgtc attgacgttt gtcccaagaa gctgccgctc tccattcaaa
     1141 tcgccttcct cagaagaaaa atggtttccg tgggtctgag tgatttcttc ttaaagctat
     1201 gattgagtgc acgggccgga aatagatctg ccccgctcag ccacgctgct tgccactctc
     1261 aatcgcgata aaagccaccc cggatttttg caccggtgcg aagaggatat ccaaaaacac
     1321 agtgaatacc gcacggacct ttacggtaca cgcgttgcga tacggactcg gcagaagcgg
     1381 aggtgtttat gagtaagacg atggcaaaac ccggactaat cgtttccggc ggcgtcgcgc
     1441 atgcagagaa agaaacgaag ccgctaggaa attactggaa agcgctaatc ccgattgcag
     1501 cgggagcgct actgttgttg ttgccgattc cggaagggct caagccgtat gcgtggtatt
     1561 actttgcgtt gtttgttgcg gtgatcatcg cgctgatcct cgagccgatt ccggcggcag
     1621 ccgtgggcct gattggtgta acggctgcga ccatcacgcc ctgggtcgcg gctaaacctg
     1681 cagactcgat caagtgggcg ctctccggat ttcaggacgg tacggtttgg ctgattttcg
     1741 tggcctttat gtttgcgctg ggctatgaaa agaccggctt gggccggcgc gttgcgttaa
     1801 atctggtcaa atggctgggt aagagaacac tcggactcgg ctatgccgtc gcgctggcgg
     1861 atctcgtgct ggcgccgttt acaccgtcga atactgcgcg cagcggcgga acgatctttc
     1921 cagtgatcaa gaatattccg ccgctctacg gatctgagcc gggtccgacc gcgcgtaaga
     1981 tcggctccta cctcatgtgg acggcgttcg cgaccacgtg cttgacgagc gcaatgtttt
     2041 taaccggtct ggcgccgaac ctgttggcgc tgtcactggt caagaccacg gctaagatcg
     2101 acatcagctg gaccgaatgg ttcatcggta ttatgcccgt cggggtgttg ctgtttatct
     2161 tcttgccact gatcgtgtac aaaatttatc cacccgaggt taagagcagt ccggatgtgc
     2221 cggtctgggc gggtagcgag ctcaagaaga tgggccgcat cagcgcgaaa gaggtcggca
     2281 tggcgctgct cgctctcgtg gcactggggt tatggatttt cggcggagac ctcatcaatg
     2341 ccaccaccgt tgcgatcctg gtgctgtgtc tgatggtcat ctgccgcatc gtcgaatggg
     2401 atgacgtgct cgcaaacaaa caggcctgga acgttctggt ttggtttgcc acgctggtga
     2461 ccatggcgga tggtttgaat agagtggggt tccttaaatg gttcgcgacc ggagcggcgg
     2521 gagcattggg tggctattcg gtgatgacga tcgtgattgt gctcacggtg gtctacttcg
     2581 tcgtccatta catgttcgcg agcctcaccg cgcacaccac tgcactgctg ccggtgcttt
     2641 tgaccgcggc gatcgcagtc ccggaaatgc ccgtgaaact aatttcgctg ctcttctgct
     2701 atacgctcgg tttgatgggg atcatctctc cctatgcaac agg
//
LOCUS       DASULZ010000168         2179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_121532, whole genome shotgun sequence.
ACCESSION   DASULZ010000168 DASULZ010000000
VERSION     DASULZ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121532"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="VFY96_03450"
     CDS             complement(<1..1085)
                     /locus_tag="VFY96_03450"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605542.1"
                     /translation="MQTLGADLRRMQAECDTRNTRLIGHHDLDGSGDHMQIIKVGRYA
                     YVAHVGKSNMALSILDISDPGNPCLVRQIPHPHNTHNHKVQIVGNTLIQNSEYISYLA
                     KTGSEKPVTGLNVYNLDDPTNPKLVGFYPVGMRGVHRIWYREAPHAHIAAPVGRSRGY
                     HIVDLSDPGRPTLVGCWWPPGAKKDDADPWEVLDPAHDKFQVHGAIPYGNRAYVSCTD
                     AGVAILDIADLAAPRLISRINWCPPYGGYAHTALPLPGRGLLIALCEYSPGGRERNGD
                     KRIWVIDIREERQPVIIGSFPEPKPSRNSPWQSFHDRPVRFGPHNLHENYGNGFQSEK
                     FIFSTWFCAGMRVTDISDPDRPVEVGH"
     gene            complement(1168..1419)
                     /locus_tag="VFY96_03455"
     CDS             complement(1168..1419)
                     /locus_tag="VFY96_03455"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX5605543.1"
                     /translation="MATEIELFGVKYKEGSLDAKAAELIKFAVNLAIGHEHGAKLHHD
                     RARKQGASDDEVWETIAYAMRPVAAQVRNFAKDIVGKQQ"
     gene            1650..>2179
                     /locus_tag="VFY96_03460"
     CDS             1650..>2179
                     /locus_tag="VFY96_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605544.1"
                     /translation="MKLGACVRFTAALIVSVLSAAAFAQESVEATLAKLNALPAAERQ
                     ASLVQGAQTEKTVEWYATLPVELSAQLMTAFRKRYPFLDVKYTRGGGGRMVNRVITEH
                     RAGSNKFDVLGGTSTSHEILMKAGLIARNMTPLRKELRDGFIDADGYRVMPFTYAFVI
                     GYNTRTVPKDQRPQAY"
BASE COUNT          445 a          600 c          658 g          476 t
ORIGIN      
        1 aagtggccca cctcgaccgg ccggtcggga tcgctgatat cggtaacacg catgccggcg
       61 caaaaccagg tggagaagat gaacttttca ctttggaaac cgttgccgta gttctcgtgg
      121 agattgtgtg gtccgaaacg gaccgggcga tcgtggaaac tttgccaggg cgaatttcta
      181 gacggtttag gctcgggaaa acttccgatg atcacgggct ggcgctcttc tctgatatcg
      241 ataacccaga tgcgcttatc gccgttccgc tcgcgcccac cgggcgaata ctcgcagagc
      301 gcgatgagga gcccgcggcc gggcagcggc aacgcggtgt gagcatagcc gccgtagggc
      361 gggcaccagt tgattctgct gatgagtctc ggcgcggcta aatccgcaat gtcgagaatc
      421 gcgacgccag catcggtgca agagacgtaa gcgcgattgc cgtacggaat ggcgccgtgc
      481 acctgaaact tatcgtgtgc ggggtcgagg acttcccatg ggtccgcatc atctttcttg
      541 gctccgggcg gccaccaaca gccgaccagc gtcggtctgc ctggatccga tagatcgacg
      601 atgtgatagc cgcgcgagcg gcctacgggc gcggcaatat gggcgtgggg cgcctcgcgg
      661 taccagatgc ggtgaacccc tcgcataccc accgggtaga agccgaccag cttcgggttg
      721 gtgggatcgt cgagattgta gacgttgagc ccggtgactg gcttttcgga gccggttttg
      781 gcgagatagg agatgtactc gctgttctgg attaaagtat tcccgacaat ttgaaccttg
      841 tggttgtgcg tgttgtgcgg gtggggaatc tgacgcacca agcacggatt gcccggatcg
      901 gaaatgtcga ggatcgacag cgccatgtta cttttgccga catgggcgac atacgcgtag
      961 cgcccgacct tgatgatctg catgtggtcg ccgctgccgt cgagatcatg gtggccgatc
     1021 agccgagtat ttcttgtgtc gcactccgct tgcattcttc gcaagtcggc gccgagagtt
     1081 tgcatgactt cgttaccttc tcattcaagc agctagcgta agcgcaggaa cggagtcacg
     1141 gcgcgcagca gcagtccatc agcgagatca ctgctgcttg ccgacgatgt ccttggcaaa
     1201 gtttcgcacc tgggcggcga cggggcgcat agcgtaagcg atcgtctccc acacttcatc
     1261 atcgctggcg ccctgttttc gcgcccggtc gtggtggagt tttgcgccgt gctcatggcc
     1321 gatcgccaga ttgacggcga acttgatgag ctccgccgct ttggcgtcca atgagccttc
     1381 cttatatttc acgccgaaga gttctatttc agtcgccatg gatcacatcc tttctaaccg
     1441 cgaatgccaa ctcaataaca gaaaagcccc cggatctgtc aaacgtctcg atcgaacttg
     1501 agacttggac accggaatgt ttcccgattt tgaaattcga atttttattc ttgcaaacac
     1561 agcggcggga acaacttgcg aggtcgcacg agtttgctat agagtccgcc agcgatctca
     1621 tttcggtgac gcaacttgaa ggaggctgca tgaagctcgg tgcttgcgta cggtttaccg
     1681 cggctttgat tgtgtcggta ttgtcagccg cggcattcgc gcaggaatcg gtggaagcca
     1741 cgctcgccaa attgaacgct cttcccgctg cagagagaca agcgagcttg gtgcaaggag
     1801 cgcagaccga aaagaccgtg gaatggtacg cgactctgcc ggtcgagctg tccgcgcagc
     1861 tgatgacggc gtttcgaaaa cgctatcctt tcctcgacgt aaagtatact cgcggcggcg
     1921 gtgggcgcat ggtcaatcga gtgatcaccg agcatcgggc cggcagcaac aaattcgacg
     1981 tactgggtgg aacgagcacg tcccacgaaa ttttgatgaa ggcgggtctg atcgcgcgca
     2041 acatgacgcc ccttcgcaaa gagctccgcg acggatttat cgacgccgat ggttatcggg
     2101 tcatgccgtt tacctatgcc ttcgtgatcg gttacaacac gcgcactgtg ccgaaagacc
     2161 agcggcctca ggcctacga
//
LOCUS       DASULZ010000169         4644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_730349, whole genome shotgun sequence.
ACCESSION   DASULZ010000169 DASULZ010000000
VERSION     DASULZ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730349"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..1096
                     /locus_tag="VFY96_03465"
     CDS             221..1096
                     /locus_tag="VFY96_03465"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5605545.1"
                     /translation="MRIALGLPSRIIGATGELMLEWITRAERGPFSSVVVTDRVVSQA
                     LEPLSVLALAAGATRRIGLMTSVVIGPTRETTLLARQAATIDVLSNGRLTLGIGIGVR
                     KNDYDASGFDFHRRGRRAEEQLPILRQLWSGEAFSKEIGAIGPRSTRSVGPELLIGGY
                     VPAIVQRIAKWGDGYMAPGGGEPESMLKMWREIERAWRDTGRLGEPRWVGASYFTLGP
                     QAVEHATQYIQANYGYNPELAAKRLRTMPTSESAVEDAIKRQHDMGVDEFILRPCAEN
                     FDQMDRLADIAARTS"
     gene            complement(1124..2572)
                     /gene="glmL"
                     /locus_tag="VFY96_03470"
     CDS             complement(1124..2572)
                     /gene="glmL"
                     /locus_tag="VFY96_03470"
                     /inference="COORDINATES: protein motif:HMM:NF040745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HEX5605546.1"
                     /translation="MTSTSLAILIDFGSTFTKLVALDLDRAELLGRAQAPSTVTTHVG
                     EGLLQALALLHEHHRVFEHKPKELSVLAGRTVLASSSAAGGLRIAVVGNVPGLTVEAA
                     NQAALGAGAKVVGSTAFKLSAGQIAEIEQLQPDIILLTGGVDGGDTATILHNARVLAD
                     SALSVPVVVAGNQTVAGEVRQILQTGKNEILPVQNVMPRTGTLAVESARAAIRQLFMD
                     RITHAKGLDEVKEFVSVVLPTPMAVLEGVRLGADGTSDEKGWGDMLLVDVGGATTDVH
                     SIGFGHPAGENVISRGLAESYAKRTVEGDLGIRFNAATILHRVGLEKLSDDLRRDFPD
                     ITVSRESLRSYIDRISHQTESVPSEKWHLAADAVLARAAADLAIARHVGRRERIVARE
                     GEAWVHSGKDLRDTRVLIGTGGVFIHNRFAAYILTPGHATDERVEALRPRNPKLFLDA
                     SYLLYAVGLLAKDHSRTALRLFKKSLHPLEMA"
     gene            complement(2664..3689)
                     /locus_tag="VFY96_03475"
     CDS             complement(2664..3689)
                     /locus_tag="VFY96_03475"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5605547.1"
                     /translation="MFFSVVFFLAITLLGISAVGAQSAKEFYAGKTITILAGSSAGGG
                     TDQTVRLLARHLERYIPGKPSIVVVNKPGAGGLIAVNELYNLRKPDGLTMSNINTGAI
                     FAVAGGNDAIKFDLRKIIYIGQALDEAQTVYVRSATPYTSLEAIRKANKEGKQPRMGA
                     QALDHTSSFVVKVAEQILGLDFQVIPGYPGTPEILLDIERGALDGRAQGTGSLLATRR
                     EWLRSGYITPLMTSRKTRDARIPKVPSILELAPAGSQGLISALSAAQNIGRALAVPPG
                     VPADRVKVLRDAFAAITKDEQFLKEAEKIGLEVGLIRGEDLNQDIDNTLSDKRMMDLY
                     RTIASAK"
     gene            complement(3743..>4644)
                     /locus_tag="VFY96_03480"
     CDS             complement(3743..>4644)
                     /locus_tag="VFY96_03480"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605548.1"
                     /translation="SESMVPFGLEKLGISQAMKEKAVDIALDVQSRTVFYQRARGADL
                     YILCGWRNQHTNVWVAPPHIKSLQDLKGKRVGISDFNSIRHWAIQIQLKKAGLDLERD
                     IEWVRLGVNARFHVDAIRNGKVECAPVPPWSAEDLKKEGCNALVSPADQYPDGRPERI
                     IAATGRILEESPDLVKSFLRAMIRSYWFIRDMPKNYEYVNNLEKRLRLKSADPEERVV
                     THLRTPRDLEAMPFPLNGRATGFEDMLKEEERLGELNYEVPPIKEVAAQDLVDEAFKE
                     LRARKELAEEYERVSEVEKRWGY"
BASE COUNT          971 a         1397 c         1274 g         1002 t
ORIGIN      
        1 gttcgcgtgc cacaccagtc gaagcgcctg cggcgtgtgc gcgaacgagt cgcgaatttt
       61 ccgcgaataa tccgacaaac gattgactag attttgcgtc acgggttgaa gataacacga
      121 actcgcaagt tttgccggtc aaacgccaac ggaaccatcg attgacacgc accgcgctcg
      181 atcgctactt tcgaagccaa cctttagttt aggaggaaca atgcgtatcg cactgggact
      241 gccgagccgg atcatcggcg caaccggaga attgatgctc gaatggatta cccgcgccga
      301 gcgcggtcct ttctccagcg tcgtcgtcac cgatcgagtc gtatcgcaag cgctcgagcc
      361 gctctcggta ctcgcgttgg ctgcgggcgc aactcggcgc atcggcctga tgaccagcgt
      421 ggtcatcgga ccgacgcgcg aaaccacttt gctcgcgcgc caggccgcca ccatcgatgt
      481 gctgtccaat gggcgtttaa ccctcggtat tggcatcggt gtgagaaaaa atgactatga
      541 tgcgagcggc ttcgattttc atcgtcgtgg ccgacgagcg gaagagcagt taccgatctt
      601 acgccagctc tggtccggcg aagcgttttc gaaggaaatt ggcgcaatcg gcccacgctc
      661 gacccgatcc gtcgggccgg aattgctcat cggcggttac gtcccggcga tcgtgcagcg
      721 cattgcaaaa tggggcgatg gctacatggc gcccggcggc ggcgaaccgg aaagcatgct
      781 caaaatgtgg cgcgagatcg agcgcgcgtg gcgcgacacc ggacggcttg gcgagccacg
      841 ctgggtgggc gccagctatt ttactctcgg gcctcaggcg gtcgagcacg ccacgcaata
      901 catccaggca aattacggat acaacccgga gctcgcggcg aaacgcctgc gcaccatgcc
      961 gacttcggaa agtgctgttg aagacgctat caagcgtcaa cacgacatgg gggtcgacga
     1021 attcattctc cgcccctgtg cggagaattt cgaccaaatg gatcgccttg ccgacatcgc
     1081 cgctcgaacg agctgaaaaa gctcggatgg gacaagcgcg gctttatgcc atctcaagag
     1141 gatgtagaga ttttttgaac aaccgcaacg ccgtgcggct gtgatctttg gcaagcaagc
     1201 cgacggcata gagtaagtac gatgcatcca gaaacagctt agggtttcgc ggccgtaacg
     1261 cttcgacccg ctcgtcggtg gcatgaccgg gtgtaagaat gtaagccgca aaacggttat
     1321 gaatgaacac accgcccgtg ccgatcagga cgcgcgtatc gcgcaaatct ttgccggaat
     1381 ggacccaagc ctcgccttcg cgcgcaacga ttcgctcgcg ccgtccgacg tggcgagcga
     1441 tcgcgagatc ggccgcggcg cgcgccagca ccgcgtctgc cgcgagatgc catttttcac
     1501 tgggcacgga ctcggtttga tgactgattc gatcgatata actgcgcagc gattcccttg
     1561 agacggttat gtcggggaag tctcgccgca aatcatcgct aagcttctcc agcccgactc
     1621 gatgaagaat ggtcgccgca ttgaaacgga ttcccagatc accttccacg gtccgcttcg
     1681 cataagattc ggcgaggcct cgcgagataa cgttttctcc ggcaggatgt ccaaatccga
     1741 tcgaatgaac atcggtagtc gcgccgccaa catcaaccag caacatgtca ccccatcctt
     1801 tttcgtcgct tgtgccgtcg gcgccgagcc ggacaccttc gagcacggcc atgggcgtcg
     1861 gcaacaccac tgagacaaat tcttttactt cgtcgagtcc cttggcatga gtaatacgat
     1921 ccatgaatag ttggcgaatc gccgcgcgcg ccgattccac cgccaaggtt cctgtccttg
     1981 gcataacatt ttgcaccggc aaaatttcgt ttttgccggt ttgaagaatt tgtctcacct
     2041 caccggcgac cgtttgatta ccggcgacaa cgaccggcac cgaaagcgcc gaatcggcaa
     2101 gcacccgcgc attgtgcaga attgtcgctg tgtcgccacc gtccacgccg ccggtcaaga
     2161 gaattatatc cggctgcaac tgctcgatct cggcgatttg cccggccgaa agcttgaatg
     2221 cggttgaacc gacgaccttg gcgcccgccc cgagggcggc ttgattcgca gcctccacag
     2281 tcaatcccgg aacattgccc acgacagcaa ttcgcaaacc gcccgcagcg ctgctcgagg
     2341 cgagaacggt tcttccagcg agcaccgaga gctctttcgg cttatgctca aatacacgat
     2401 gatgctcgtg caagagcgca agagcttgca gcaatccctc acctacgtgg gttgtcacgg
     2461 tggatggcgc ttgggctcgg ccgagcaact cggcgcggtc gagatccagc gcgaccagct
     2521 tggtgaaagt actaccgaaa tcgatgagaa tggcgaggga agtcgaagtc ataggcagtt
     2581 gccagctacc agttcacaat gatcagtgga cttcgctcgt ttgacttgct ggcaactgac
     2641 gacgacatgc taaccactgt ttctcacttc gccgaggcga tcgtgcgata tagatccatc
     2701 atacgtttgt cggaaagcgt gttgtcaatg tcctggttga gatcttcgcc gcggatcaaa
     2761 cccacttcca ggccgatctt ttctgcttct tttaaaaact gctcgtcttt agttatcgcc
     2821 gcaaaggcat cgcgcaatac tttcacccga tccgccggga caccgggcgg gacggcaagc
     2881 gcgcgtccga tattttgcgc cgcggataac gcgctgatca atccctgact gcccgctggt
     2941 gccaactcca gaatgctcgg taccttgggt atccgggcat cgcgcgtttt tcgcgacgtc
     3001 atcagcggcg taatgtaacc gctcctgagc cattcccgtc tggtggccag caacgacccg
     3061 gttccctggg cgcgcccgtc gagggcaccc cgctcaatgt cgagaagaat ttccggcgtc
     3121 cccggatacc cgggaatcac ctgaaaatcc aggccaagaa tctgttcggc cactttgacc
     3181 acgaagctgc tcgtgtgatc gagcgcttga gcgcccatgc gcggctgctt accctctttg
     3241 ttcgctttcc gaatcgcttc caggctcgtg tagggagtgg cagaccggac gtaaaccgtc
     3301 tgtgcctcat cgagcgcctg gccgatgtaa ataatttttc tgaggtcgaa cttgatcgcg
     3361 tcgttccctc ctgcaacggc aaagatggcg cccgtattga tattgctcat cgtcaaaccg
     3421 tccggtttgc gcaggttgta aagctcgttg accgcaatca ggccgccggc gccgggtttg
     3481 ttaacgacca cgatcgaagg ttttccggga atgtaccgtt cgaggtggcg cgccagtaat
     3541 cgcacggttt ggtccgtgcc gccaccggcc gaggagccgg ctagtatcgt gatggtttta
     3601 ccggcgtaaa actccttcgc tgactgtgca cccacggctg aaatccccaa cagcgtgatc
     3661 gctagaaaaa agacaacgct aaagaacatc cgattgactc gaatcatacg tttccaaaat
     3721 ttaagcaatt tgccgctttc gcctagtagc cccaacgctt ctcgacctcg ctcacgcgct
     3781 cgtactcttc cgcgagttcc tttcgcgcgc gcaattcttt gaaagcttcg tcaactagat
     3841 cctgcgcggc gacttccttg atcggcggca cctcgtaatt gagctcgccg agccgctctt
     3901 cttccttgag catgtcctcg aatccagtgg cgcgcccgtt cagtggaaac ggcatcgcct
     3961 ccagatcgcg cggcgtgcgt aagtgcgtca ccactcgttc ttcgggatcg gcgctcttga
     4021 gccgcagccg cttttccaga ttgttcacgt attcgtagtt cttgggcatg tcgcgaatga
     4081 accagtagct tcgaatcatc gcacgcagaa agctcttcac tagatccgga gactcttcga
     4141 gaattcgccc ggtggcggca atgatcctct cggggcggcc atcgggatat tgatccgcag
     4201 gcgaaaccag cgcgttgcac ccttccttct tgaggtcctc cgcgctccat ggcggcaccg
     4261 gagcgcattc gactttgccg ttgcggatcg cgtccacatg aaaacgcgca ttgacgccga
     4321 gtcgtaccca ctcgatatcg cgctcgagat ccaatccggc ttttttgagc tgaatctgga
     4381 ttgcccagtg acgaatgctg ttgaaatcgc tgatgccgac tcgtttccct ttgagatcct
     4441 gcagcgattt gatatgcggg ggcgccaccc agacgtttgt gtgctgatta cgccagccgc
     4501 aaagaatgta gagatccgcg ccacgggcgc gctggtaaaa aaccgtccgc gactgaacgt
     4561 cgagcgcaat atccacagct ttttctttca tcgcctgtga aatacccaat ttttccagac
     4621 cgaacggcac catcgattcg ctca
//
LOCUS       DASULZ010000170         3174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1339735, whole genome shotgun sequence.
ACCESSION   DASULZ010000170 DASULZ010000000
VERSION     DASULZ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339735"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VFY96_03485"
     CDS             <1..267
                     /locus_tag="VFY96_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605549.1"
                     /translation="LAFQIADDILDANAGVAVEERAESSRIERKKATYPSVVGLVTAR
                     ERLAELLQLSLRELDFFALRGRPLAAIARYIVGRAIHLDDARTH"
     gene            276..2201
                     /gene="dxs"
                     /locus_tag="VFY96_03490"
     CDS             276..2201
                     /gene="dxs"
                     /locus_tag="VFY96_03490"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX5605550.1"
                     /translation="MPNPKRLLERVEDPADIRGFTIEELNQLAAEIRDEVISVVSEVG
                     GHFASTLGAVELTLALHYVFRTPEDRIVWDTGHQAYAHKLICGRRDRLSTIRQFGGLS
                     GFLSREESQYDVFGAGHAGTSISAALGMVEAKVLEGSDRKVVAVISDGGLSAGLTYEG
                     LNSAGHLDRDLIVVLNDNEHFIDPRVGAVSSFLSKQFTTNFGVRIQKNLSHLLKSLPS
                     GENLKLAARKFRDSFLSLVTPGFLFESLGFQYVGPIDGHNIGEMIVTLENVKKIEGPT
                     LVHVLTRKGKGYGPAEADPIKFHGVTPFHVLTGKAKKEKGPIPTYTEVFGQSLLRVAK
                     NHPKVVGITAAMGSGTGIDKLAREIPDRTYDVGIAEQHAVTFAGGMATEGYIPVVAIY
                     STFLQRGYDEILHDVCLQNLHVGFALDRGGLVGADGPTHHGVFDFAYMRSIPNIVIMA
                     PKDENELQHMLKTAIDHNGPISLRYPRGEGWGVSMDDEMRPLEIGKAELLRDGKDVVF
                     AAIGQTVLPALQAAEDLAALGIDAAVVNARFVKPLDRNLFRDLLFRVPRLITVEDHVI
                     AGGFGSALIEFLADEGITGVAIKRLGVPDRFIPHGTQEELRKLCGFDKDAIAQAGLQM
                     VRRGRKRSNEGWERGSA"
     gene            2180..2914
                     /locus_tag="VFY96_03495"
     CDS             2180..2914
                     /locus_tag="VFY96_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX5605551.1"
                     /translation="MGTRERLDLLLLERQLVASREEGRRRILAGEVLVNDQAVTKAGS
                     FVELGARIDLKPAPKYVSRGGFKLEKALQEFAISVEGKTTLDIGASTGGFTDCLLAHG
                     AARVFAVDVGYGQLDWRLRNDPRVVVLEKTNIRHLDRRSLPHNVELATIDASFISLKL
                     ILPCVKALLPVTHEVIALIKPQFEVGKGKVGKGGVVRSAAEHARVIDEIEKAAAATGY
                     RSRGIVESPLLGPKGNKEFLLYLDMS"
     gene            2959..>3174
                     /locus_tag="VFY96_03500"
     CDS             2959..>3174
                     /locus_tag="VFY96_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX5605552.1"
                     /translation="MASLRKIAHRGASGRYPENTRLAFTKAIEAGADMIELDCQLSRD
                     GHVVVFHDERLNRVGRSRGTVRSKTLEQ"
BASE COUNT          677 a          857 c          951 g          689 t
ORIGIN      
        1 ttggcgttcc aaattgccga cgatattctg gatgctaacg ccggtgttgc cgtcgaagag
       61 cgcgccgagt ccagccgcat agagcgaaaa aaagccacct atccatccgt ggtcggcctg
      121 gtgaccgcgc gagaacgact cgcagagcta ctgcaacttt ctcttcgcga gctcgatttt
      181 tttgcattac gcggccggcc gcttgccgca attgcccgct acatcgtagg tcgcgcaatt
      241 catctcgatg acgcacggac gcactgagga ggtctatgcc taatccgaaa cgcttgttgg
      301 aacgggtcga agatccggca gacatccggg gctttactat tgaagagctg aatcagctcg
      361 ctgcggagat tcgcgacgaa gtcatttcag tcgtttcgga ggtgggcggg cacttcgcct
      421 cgactctcgg cgcggtcgag ctcacgttgg cgcttcacta cgtgtttcgc acgcccgagg
      481 atcgcatcgt ttgggatacg ggccaccaag cctacgctca caagctgatc tgcggtcgcc
      541 gtgaccggct ctcgacgatt cgtcagtttg gcggcttaag cggatttttg agccgggaag
      601 agagccagta cgatgttttt ggcgcgggtc acgccggcac atcgatttca gctgccctcg
      661 gcatggtcga agccaaagtc cttgaaggct ccgaccgtaa agtggtcgcg gtgatcagcg
      721 acggcggctt gagcgcagga ttgacgtacg aaggactcaa ctcagcgggt catcttgacc
      781 gcgatctgat cgtcgtgctc aacgacaacg agcattttat cgacccgcga gttggcgctg
      841 tttcatcatt tttgagcaag cagttcacga ccaatttcgg tgtgcggatc caaaagaatc
      901 tgtcgcatct gctgaagagc ctgcccagcg gtgaaaatct gaaacttgcg gcgcggaaat
      961 ttcgcgattc ctttttaagc ctggtgacgc ccggttttct cttcgagagt ctcggttttc
     1021 aatacgtcgg tcccatcgat ggtcacaata tcggcgaaat gatcgttacc ttggagaacg
     1081 tcaagaaaat cgagggcccg acgctggtgc acgtgctgac cagaaaaggc aagggttatg
     1141 gacctgcgga agccgatccg atcaagtttc acggggttac gccgtttcac gtgctcaccg
     1201 gcaaggcaaa aaaagagaag gggccgattc cgacttatac agaagttttc ggtcagtcgc
     1261 ttctgcgcgt cgcgaaaaat catcccaagg tcgtcggcat caccgcagcg atgggtagcg
     1321 gcacaggcat tgacaagctg gcacgggaga ttcctgatcg cacctacgat gtcggcatcg
     1381 ccgagcagca cgccgtgacc ttcgccggcg gaatggcgac cgaaggttat attccggtgg
     1441 tggcgatcta ttcgaccttc ttgcagcgcg gctatgacga aattctgcac gacgtctgtt
     1501 tgcaaaatct tcacgtggga ttcgctctcg atcgtggcgg cttggtcggt gcggacgggc
     1561 cgacccacca tggcgtgttc gattttgcat atatgcggtc gattccgaac attgtcatca
     1621 tggccccgaa ggacgaaaac gagttgcagc acatgctcaa aaccgccatc gatcataacg
     1681 ggccgatttc gctccgttac cccaggggcg agggttgggg cgtgtctatg gatgatgaga
     1741 tgcggccttt ggaaatcggc aaggcggaac ttttgcgcga tggcaaagac gtagtatttg
     1801 cggcgattgg ccagacggtg cttccagcct tgcaagctgc ggaagatttg gccgcactcg
     1861 gcatcgacgc cgcggtggtg aacgcgcgtt ttgtgaagcc actggatcgc aatttgttcc
     1921 gtgatctgtt gtttcgagtg ccgcgactca tcaccgttga ggaccatgtg atcgcgggcg
     1981 gtttcggcag tgccttgatc gagtttttgg ccgatgaagg aattaccggg gtcgcgatca
     2041 aacggctcgg cgtaccggac cgtttcattc cccatggcac ccaggaggag ctgagaaagc
     2101 tttgcggctt cgataaggat gccatcgccc aggcgggctt gcagatggtg cggcgcggca
     2161 gaaaacgaag caatgaagga tgggaacgcg ggagcgctta gatctcttgc tgctggagcg
     2221 ccagttggtc gcgagccgtg aggaaggacg gcgcaggatc ctcgcgggcg aggtgcttgt
     2281 caacgatcag gcggttacca aagccggcag tttcgtggaa ctcggcgcgc ggatcgatct
     2341 taagcctgcg ccaaaatacg ttagccgcgg cggtttcaag ctcgaaaaag ccctgcaaga
     2401 gtttgccatc tctgtcgaag gtaagacgac cctcgatatc ggcgcatcga caggcggatt
     2461 caccgactgt ttgcttgccc atggcgcagc gcgcgtgttc gcagtggatg tgggctatgg
     2521 ccagctcgat tggagattgc gcaacgaccc gcgcgtggtg gtgctggaaa aaaccaatat
     2581 tcgccacctc gatcgacgct ctctgccgca caatgtcgag cttgcaacca ttgacgcatc
     2641 attcatttcc ctgaaactga ttctcccttg tgtcaaagcg cttttgcccg taactcatga
     2701 agtgattgcc ctgatcaagc ctcagttcga ggtcggcaag ggcaaagtgg gcaagggtgg
     2761 cgttgtccgt tccgccgcgg agcacgcgcg agtgatcgat gagattgaaa aagccgcagc
     2821 ggcaactggc tacaggagcc gcggcatcgt cgaatctccg ctgctcggac caaaaggcaa
     2881 taaggaattt ttgctttatc tcgatatgtc ctgagcggta tcgcctacca caaacagcgc
     2941 gcgtcgacca tagttgccgt ggccagtctc agaaaaatag cccatcgcgg cgcttccggg
     3001 cgctatccag aaaacactcg cttagcgttt acaaaggcca tcgaagcggg cgccgacatg
     3061 atcgagctcg actgccagct cagccgtgat ggccacgtgg tggtatttca cgacgaacgc
     3121 ttgaaccgcg ttggacgcag caggggaaca gttcgcagca agacgctcga gcaa
//
LOCUS       DASULZ010000171         2713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_20686, whole genome shotgun sequence.
ACCESSION   DASULZ010000171 DASULZ010000000
VERSION     DASULZ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20686"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..145)
                     /gene="rplB"
                     /locus_tag="VFY96_03505"
     CDS             complement(<1..145)
                     /gene="rplB"
                     /locus_tag="VFY96_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837367.1"
                     /note="one of the primary rRNA-binding proteins; required
                     for association of the 30S and 50S subunits to form the
                     70S ribosome, for tRNA binding and peptide bond formation;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX5605553.1"
                     /translation="MAIKTYQPTSAGMRFRTGLSFDEISKTGAEKGLRERLNRSGGRN
                     NTGR"
     gene            complement(166..456)
                     /locus_tag="VFY96_03510"
     CDS             complement(166..456)
                     /locus_tag="VFY96_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX5605554.1"
                     /translation="MRSPQDVIRAPLISEKGTQLTESANQVLFKVSPGANKIEVKHAV
                     ESLFKVTVVQVRMARYLGKMRRIGRNMGRRSDWKKAYVTLKEGDKIDFFGGA"
     gene            complement(453..1037)
                     /gene="rplD"
                     /locus_tag="VFY96_03515"
     CDS             complement(453..1037)
                     /gene="rplD"
                     /locus_tag="VFY96_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX5605555.1"
                     /translation="MIEPTLNEAIFGAKPRTHLLHQAVVMQLANRRTGSAATKSKGFV
                     SGGGKKPWRQKGTGRARAGSIRSPIWVGGGTVFGPQPRDYSYRMPRTARREALLSALS
                     LKKRENKIIVVDKLELEQAKTKLMVQALAELKVASALLVIPQADAKIERSARNLPNVK
                     VLRVEGLNVYDLLRYEHLILTDEALKILQEKLAA"
     gene            complement(1055..1687)
                     /gene="rplC"
                     /locus_tag="VFY96_03520"
     CDS             complement(1055..1687)
                     /gene="rplC"
                     /locus_tag="VFY96_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX5605556.1"
                     /translation="MTGLIGRKLGMTQFYNAEGNVVPATVVETGPCIVVQKKLATTDG
                     YNALQVGFGSKKNQRVNKAAQGHMAKAGKGAFEVLREFRLEDVGSYEIGQEIKAGDLF
                     KVGDRIDVSGISKGHGFSGVIKRWSFSGFPGSHGTHEYFRHGGSIGNRSYPGRVRKGK
                     KMAGHWGNETVSTQNLEVVAIRAEENLLLVRGAVPGSKRGIVILRPAVKR"
     gene            complement(1726..2034)
                     /gene="rpsJ"
                     /locus_tag="VFY96_03525"
     CDS             complement(1726..2034)
                     /gene="rpsJ"
                     /locus_tag="VFY96_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX5605557.1"
                     /translation="MNEKIRIRLKAYDHRVLDQSVGEIVETVRRTGGRVAGPIPLPTR
                     IERFTVNRSPHVDKKSRDQYEIRTHKRLLDILEPTQQTIDALGKLDIAAGVDVEIKLQ
                     "
     gene            complement(2031..>2713)
                     /gene="fusA"
                     /locus_tag="VFY96_03530"
     CDS             complement(2031..>2713)
                     /gene="fusA"
                     /locus_tag="VFY96_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365698.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX5605558.1"
                     /translation="ANVGKPQVAYKETVRKKVEQQGRFVRQTGGRGQYGDVWITLEPQ
                     QPGAGFEFIDAVKGGSIPREYISAVEKGIKEATENGPLAGYPVVDVKATLFDGSYHDV
                     DSSEIAFKIAGSMAFKGAARKANPVLLEPIMAVEVVVPEEFMGDVIGDISSRRGKVLG
                     MDTRPAAQAIDARVPLAQMFGYATDLRSMTQGRATYTMQFSHYEPVPAAVAEGIIAKF
                     DGKSDDNV"
BASE COUNT          527 a          846 c          658 g          682 t
ORIGIN      
        1 ctcggcccgt gttattacgc ccgccgctac ggttgagccg ctcacgtaag cctttttccg
       61 ctccagtctt gctgatttca tcgaagctta gtccggtccg aaagcgcatg cccgcagagg
      121 tcggttgata agttttgatc gccatcttga ttctcttagc tatgtctatg cgccgccaaa
      181 aaagtcgatt ttgtcgccct ctttaagcgt cacgtaagcc tttttccaat cggagcgccg
      241 ccccatgttt ctgccaatgc ggcgcatctt gccgagatat cgcgccattc ggacctgcac
      301 caccgtcact ttgaacaagc tctcaacggc atgcttgact tctatcttat tcgcgcccgg
      361 actgactttg aacagcacct gattcgcgct ttcggtcagc tgggtgcctt tttccgagat
      421 caggggcgct cgaatgacat cctgcgggct cctcatgccg ccagcttctc ctgtaaaatt
      481 tttagggcct cgtcagtgag gatcaggtgc tcgtaacgca gcaaatcgta aacgttcaaa
      541 ccttccaccc gcagcacttt aacattcggc agattgcgcg ctgagcgctc tattttcgcg
      601 tcggcttgcg ggatgaccag caatgcgctc gcgaccttga gttcggccag agcctgcacc
      661 atgagtttcg tcttcgcttg ttcaagctcc agtttatcga cgacgataat tttgttttcg
      721 cgtttcttga ggctcaacgc cgaaagtaat gcctcccgcc gcgctgttcg cggcatacgg
      781 taagagtagt ctcgcggttg aggcccgaat acagtgccgc ccccgaccca gatcggcgac
      841 cgaatactcc ctgcgcgcgc gcgtccggtg cccttttgcc gccacggttt tttaccgccg
      901 ccgctcacaa aaccctttga ttttgtcgcc gcgctacctg tccgtcgatt ggcgagctgc
      961 atcacgaccg cctgatgcaa cagatgcgta cgcggcttgg cgccgaaaat cgcttcattc
     1021 agagtcggct caatcacgtg aacttccatt ttgtttacct tttgacggca gggcgcaaaa
     1081 tgacaatccc gcgcttggac cccggcaccg cccctcggac gagcagcaaa ttttcctccg
     1141 ctcgaatcgc gacgacttca agattctgcg tcgatacggt ttcatttccc cagtgaccag
     1201 ccatcttttt tcccttgcgc acccgaccag gataagaccg attcccgatg gagccgccgt
     1261 gacggaaata ttcgtgggtg ccgtgggaac cggggaagcc gctgaacgac cagcgcttga
     1321 tcacgcctga gaacccgtgg cccttggaaa ttcctgaaac gtcgatccga tcgccaactt
     1381 tgaacagatc ccccgctttg atttcttgac cgatctcata cgagccgaca tcctcgaggc
     1441 gaaattctcg gagcacctcg aaggcgcctt tgcctgcttt ggccatatga ccttgcgcgg
     1501 ctttgttgac ccgctggttt ttttttgatc cgaaaccgac ctgcaacgca ttgtagccat
     1561 ccgtcgtcgc cagttttttt tgcacgacga tgcagggacc ggtttccaca acggtggccg
     1621 gaaccacatt tccttcggcg ttgtaaaatt gcgtcatgcc gagttttcta ccaatcagtc
     1681 ctgtcatatt gcgttccaat cacgtcatgc tggctcgttt tcgcactatt gaagcttgat
     1741 ttcgacatcc acccctgcag cgatgtccag tttgccgagc gcgtctatgg tttgttgcgt
     1801 cggttccagt atatccagca atcgcttgtg cgtgcggatt tcgtactgat cgcgcgattt
     1861 tttatccaca tgaggcgacc ggttgaccgt gaacctctcg atccgcgttg gtagcgggat
     1921 cggaccggcg actcgacctc ctgtgcgtct taccgtctcc acgatttctc ccaccgattg
     1981 atccagcacg cggtggtcgt aggctttgag ccgaatcctg atcttttcat tcatacgtta
     2041 tcatccgact tgccatcaaa tttggcgata attccttcgg ccaccgccgc cggaactggc
     2101 tcgtaatgag aaaactgcat cgtataagtc gcccttccct gggtcatcga ccgtaggtcg
     2161 gtggcataac cgaacatttg cgccaacggc acccgtgcat caatcgcctg cgcggccggt
     2221 cgagtatcca tgcccaaaac tttaccgcgc cgcgaactaa tatcaccgat gacatcgccc
     2281 ataaactcct cgggcacaac cacttccacc gccatgatgg gttcgagtaa caccggattg
     2341 gccttgcgcg cagctccctt gaacgccatg gaaccggcga tcttaaatgc aatctcagat
     2401 gagtcgacgt catgatagct gccatcgaac agggtcgctt tcacatcgac cacaggatag
     2461 ccggccaagg gaccgttctc cgtcgcctct ttgatcccct tctcaaccgc ggaaatgtat
     2521 tcacgcggaa tcgagccgcc cttaacggcg tcaataaatt caaatcctgc gcccggttgt
     2581 tgcggctcaa gagtgatcca gacatcgcca tactggccgc gcccgccagt ctgccgcaca
     2641 aatcggccct gctgctcgac ttttttccgg acggtctctt tgtacgcgac ctgcggctta
     2701 cccacattgg cac
//
LOCUS       DASULZ010000172         6554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_832253, whole genome shotgun sequence.
ACCESSION   DASULZ010000172 DASULZ010000000
VERSION     DASULZ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6554
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832253"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1717
                     /locus_tag="VFY96_03535"
     CDS             <1..1717
                     /locus_tag="VFY96_03535"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5605559.1"
                     /translation="PLRLFSSENGTVEAVGPDGTRRLFGISRLKNSVGGETTYAAVDL
                     PVDIAFARTREILIHQLIALAILSAIILGSTWFGTDILVLRRIRDIIAATRKIAAGDL
                     RARTILVHDNTELGQMAQAFDHLAESLERREAEAVHSSKQIHQQRQQQEALYDLNRGI
                     TSTLDVESVLGILLDHIALLFSSYTVTVSWINPQTRTLETIASREGTGSELSNGDLLG
                     TESLPLLVLQRQTLLSIADGRADAGAAGPNIFLPHGWVSYLGLPMMVKNEALGVLSFY
                     SRNRHEFTRDETTFLNALVNEAAIAIHNSQLFEQTRQQALELEKSNKIKDEFLGVMSH
                     ELRTPLNIIMNYSEALRMGTFGEISPDQVRGTDKIRQQANHLLSLINGILEITKIESG
                     TVSVEAHSVDLNIFMAELKSDYMLPLENGVALVWADTGELPSMITDQLKLKQIVTNLI
                     NNAIKFTEHGSVTVSAGISPDKSGVQIHVSDTGPGMPSELLPFIFDKFRQIDSATTRN
                     YSGAGLGLYIVKNFVSILGGTIDVRSKVGEGSVFTVSFPVALDSREAEKAPTLPNTAE
                     LCTPD"
     gene            1730..2551
                     /locus_tag="VFY96_03540"
     CDS             1730..2551
                     /locus_tag="VFY96_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605560.1"
                     /translation="MRISIFWRLVLSHIGVLLLSGVACFYSIMQLGILSGGARSALDT
                     NHRMIAYQEGLTEAFLSEVRYGGKYLISHTEARHDQLLQFKREFVNYLEMLKRLGQLE
                     SSSLSAIERLHDQYHELVDREVTYIRANQTYAQSRYQQERDKIVESTVGELDVLKTQL
                     RTRLQTKLESIDQAARTARKISIATTVVVLIVGILISLRVSRSLDGSVIRAAPATAGA
                     PGIAARLVERTSLTERVRKRLMRQAHHLTALSESLMAARSGRAGRSKSPTLRKGN"
     gene            2552..3109
                     /locus_tag="VFY96_03545"
     CDS             2552..3109
                     /locus_tag="VFY96_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605561.1"
                     /translation="MSRLAKTLKIFQAAIRRLRGFVGLILLSACFIVSCTTGDRGGSS
                     DSALGPIVQPPTDFETALKQNQAALIEAKGAEDLALYNIGVILAHPSNPKRDQTKAIN
                     SFKVLVTEYPRSIYAEPSKVWIDVLEQQRKLKDERQKLAEEKRGLMREKEMLVQERQK
                     LYYDNEKSQQLDMEIEKRRRQSLRR"
     gene            complement(3263..5059)
                     /locus_tag="VFY96_03550"
     CDS             complement(3263..5059)
                     /locus_tag="VFY96_03550"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5605562.1"
                     /translation="MAEAKGGAEIWAAGIGRKSNAQYGSDLMVEVLRELGIKYIALNP
                     GASYRGLHDSLVNFEGGKGPELIMCTHEEIAVALANGYARATGEIMATGLHDIVGLQH
                     ASMAIFNAWCDRTPILNLGGGGPLDTTNRRSTDWVHTALVQGNAVREYVKFDDQPNSV
                     DAVAESFLKAYRIAMTEPKGPVYICLDTDVQEAKISKPMIVPHAQLFRPAPGPGANPE
                     SLRNASRLLAEAQWPVIIAGEVGRNPKALPPLLDLAEALGAAVIDADGRFAFPSTHPL
                     NLTNAREEALRGADVVLALDVPSLGVPLGPSVRERGNFAPIIAPSCKVIHITLLDLER
                     QSWVSDNMWLLPVHVPMAADTAVVLPQLLDQVRERLSANKDSAKQVHERRGNIEGIYR
                     DTRKKSQEWMEKTWNEKPISQARLFGEINKRVQEKSWALVSSHGRRWREAIEVTEPAH
                     GMGGGRGGGVGYGLPSSIGAALGFKESGRLCVGILGDGDFLMTSNSLWTASKYQIPLL
                     AVVLNNRSYYNDEEHQERMARARQRPVENKGIGIRIEEPAPDLATIARGLHVDGFGPI
                     TDPDQLGPTLDKAIEIVESGKPAVVDVITQPR"
     gene            complement(5205..6167)
                     /locus_tag="VFY96_03555"
     CDS             complement(5205..6167)
                     /locus_tag="VFY96_03555"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5605563.1"
                     /translation="MRILITGGLGVNGAVAARLMVQDGLRPVLMDNRMDLTLMADIKD
                     RVDIEIGDICDRGRLENIVDRHQITHMAHLAALMPEPAEADPRLAVKVGLDGTINVLE
                     TARQKRITRIVFTSSKAAYGEITGEYAPPTCKPVREDYPKRPADLYGSIKVCCEELGR
                     YYRETYGIEFIALRFVSIYGPGKEARHGPLSFYGQLIEKARSGESWVIAQGGDQLNDA
                     VYVGDVGRAVYLALKAAAPNEWIFNIGTGKASTPRDFLHAAAKLFPKHKIELGPGPSK
                     LGRSKQSYCVFDISAAERNLGYEPAYDVARGVRDYVATLERLGR"
BASE COUNT         1473 a         1904 c         1744 g         1433 t
ORIGIN      
        1 accgctgaga ctcttttctt ccgaaaacgg aactgtcgaa gccgtcggtc cggacggaac
       61 tcgccggttg tttggcataa gccggttgaa aaactcggtc ggcggagaaa caacgtatgc
      121 cgccgtcgat ttaccggtag acatagcctt cgcgagaacc cgggaaattc ttattcatca
      181 gttaatcgct ttggccatcc tctcggcgat cattcttggt tccacgtggt tcggcacgga
      241 tatcctggtt ctgcgccgga tccgcgacat cattgcagcg acgagaaaaa ttgccgcggg
      301 agacttacgt gcgcggacaa tcctagtcca tgacaatacc gagctcgggc aaatggcgca
      361 ggcgttcgat catttagccg aatctttgga gaggcgcgaa gcggaggcgg tgcattccag
      421 caagcaaata catcagcaac gccaacaaca ggaagcgctt tacgatctca atcgcggcat
      481 cacttcgacg ctggatgtgg aaagcgttct gggaatcctc ctcgatcaca tcgcgctgct
      541 tttctcttcg tataccgtga ccgtgagctg gatcaaccca cagaccagga ctcttgaaac
      601 gatcgcatct cgagaaggaa caggaagcga gctctcgaat ggcgatttgc tcggcaccga
      661 gtcgctgccg ctgctggttt tgcaacggca gactctgctc agcatcgccg atggccgggc
      721 ggatgccgga gccgcgggtc cgaatatctt tctccctcac ggctgggtgt cgtaccttgg
      781 ccttccaatg atggtcaaga acgaagcgct cggtgttctt tcgttttaca gccgcaatcg
      841 ccacgaattc acccgggacg aaactacttt tctaaacgcg cttgtcaatg aagcggcgat
      901 tgccatccac aattctcagc tctttgagca aaccagacag caggctctgg agttggaaaa
      961 atcgaacaaa atcaaagatg agtttctcgg cgtcatgtcg cacgaattac gcacacccct
     1021 caatatcatc atgaattact ccgaggctct acgcatgggc accttcggtg agattagccc
     1081 ggaccaagtg cgcggcacgg acaaaatccg gcaacaagca aatcacctgc tctcgttgat
     1141 taatggcatc ctggaaatca caaaaataga aagcggcaca gtctcggttg aagcacattc
     1201 ggtcgatctg aacatcttca tggcggagct aaaatcggac tacatgctgc cgctggaaaa
     1261 tggtgttgca ctggtatggg ccgataccgg tgagctgccg agcatgatca ccgaccaact
     1321 aaaactcaag cagattgtga caaatttaat caataacgcg atcaagttca ccgaacacgg
     1381 ttcggtcact gtctcggccg gcatttcgcc tgacaaaagt ggagtccaaa tccatgtctc
     1441 cgacacaggc ccaggaatgc cctcggaact gctgccattt atctttgata aatttcggca
     1501 aattgacagt gcgacaacga gaaattactc cggcgccggc ctcggtctct acatcgtcaa
     1561 aaactttgtc agtattctgg gcggcacgat cgacgtgcgc agcaaagtcg gtgaaggatc
     1621 ggtgtttacg gtcagctttc ccgtcgcgct ggacagcaga gaagcagaaa aagcacccac
     1681 actgccgaat acagccgagc tctgtacacc cgactaggag aaacgggtta tgaggatttc
     1741 gatattctgg cggctagttc ttagtcacat aggagttttg ctactctccg gtgtcgcctg
     1801 cttctattca attatgcaat tgggaatctt gagcggaggc gcccggagcg ctctcgatac
     1861 caaccaccgg atgatcgctt atcaggaagg cctgaccgag gcgttcctat ctgaagtgcg
     1921 ttacggcggc aaatatctca tcagccatac cgaggcccgg cacgatcaac tgcttcagtt
     1981 caagagggaa tttgtcaatt atctcgagat gctcaagcgg ctggggcagc ttgaatccag
     2041 ttcactctcg gcaatcgagc gtttgcacga ccaataccat gaactcgtcg accgtgaagt
     2101 gacatacatt cgtgccaatc aaacctatgc tcagagccgc tatcagcaag agcgtgataa
     2161 aattgtcgaa agcactgttg gcgagctcga tgttctgaaa acgcaactcc ggacaagatt
     2221 gcaaaccaag ctcgagagta tcgatcaggc tgcccgtacc gcacggaaaa tctcaatcgc
     2281 aactacggtc gtcgtgttga ttgtgggtat cttaatttcc cttagggtca gtcggagctt
     2341 ggacggttcc gtgatccgag cggcgccagc gactgcgggt gctcctggga ttgccgcgag
     2401 attagtcgag cgcacgagcc ttacagagcg ggtccggaag cgcctcatgc gccaggcgca
     2461 ccatttgacc gcattgagcg aaagtctcat ggcggcgaga agcggtcggg ctggcagatc
     2521 gaaaagcccc acattgagaa agggcaacta aatgagccgt ttggctaaga cgctgaagat
     2581 ctttcaggcc gcgattcgtc gtttgcgcgg ttttgttgga ttaatactcc tgagcgcctg
     2641 ctttatcgtt agttgcacga ctggagatcg cggtggaagt tcggattcgg cattgggacc
     2701 gattgtacag ccgccgacgg acttcgaaac tgcgctaaaa cagaatcagg cggcattaat
     2761 cgaggccaaa ggagccgagg acctggcgct gtacaatatc ggcgtaattc tggcgcatcc
     2821 atcaaatcct aaaagggatc aaaccaaggc gatcaattct ttcaaagtct tggttacaga
     2881 gtatccgcgc agcatctacg cggaaccctc caaggtatgg attgacgtcc tcgaacagca
     2941 gcgcaagctc aaggacgaac gacaaaagct tgccgaggaa aaacgcggct tgatgcgcga
     3001 aaaggaaatg ctcgtgcagg agcgacaaaa gctttactac gacaacgaaa aatcgcagca
     3061 gctcgatatg gaaattgaaa aacgacggcg ccagtcatta cgccgttaat tcgccgcagt
     3121 ctccgcagag tctatccatc ttagatcagt ccgattctct gcgttttact ttcagctcgt
     3181 gcactactcg aacatctggg ctcggtggta ctcgacgcaa tgctgcaaag catggcacgg
     3241 cttacgatac gccgtgctgc ttctatcgcg gctgggtaat gacgtcgact actgccggtt
     3301 tgccgctctc gacgatctcg atcgctttgt ccaaggtcgg gccgagctga tcgggatccg
     3361 tgatcggtcc aaagccatcg acgtgaagtc cccgcgcaat cgtcgccaga tccggcgccg
     3421 gttcttcgat gcggataccg atgcccttgt tctccacggg ccgttggcgc gcccgcgcca
     3481 tgcgctcctg gtgctcttca tcgttgtagt aagaccggtt gttgagcacg accgctagca
     3541 aaggaatctg gtacttggac gccgtccaaa gggaattcga tgtcatcaaa aaatcaccgt
     3601 cgcctaggat tccgacgcag agacggccgc tctccttgaa accgagcgct gcgccgatgg
     3661 acgaaggcaa tccataaccg acgccgccgc cgcggccgcc acccatgccg tgcgccggct
     3721 cggtcacttc gatcgcctcg cgccagcgcc gtccgtggga tgacaccagc gcccaggatt
     3781 tctcttgcac ccgtttgttg atctcgccaa acaagcgcgc ttgtgagatc ggtttttcgt
     3841 tccaggtttt ttccatccac tcctgactct tttttcgcgt atcgcgataa atcccttcga
     3901 tatttccgcg ccgctcgtgg acttgtttgg cggagtcttt attggcgctc agccgttccc
     3961 ggacctgatc gagcaactgc ggcagcacca ccgctgtatc ggctgccatt ggcacgtgca
     4021 cgggcaggag ccacatattg tcgctcaccc agctctgccg ttcgaggtcg agtaaggtga
     4081 tgtgaatcac tttgcaactc ggcgcaatga tcggcgcgaa attaccccgc tcgcgcaccg
     4141 acggaccgag cggcacgccg aggcttggaa cgtcgagcgc caacactacg tcagcgccgc
     4201 gcagcgcctc ttctcgcgca ttggtcaggt tcaatggatg cgtgctcggg aaagcgaaac
     4261 ggccgtcggc atcgatcact gccgccccga gcgcttccgc caaatcgagc agtggcggca
     4321 gcgccttggg atttcggccc acttcccccg cgatgatgac cggccattgt gcttcggcca
     4381 aaagacgcga agcattgcgc agagattctg gattcgcgcc ggggccgggc gccggccgaa
     4441 acagctgtgc atgcggcacg atcatcggtt tgctaatttt tgcttcctga acatccgtgt
     4501 ccaggcagat gtagaccggc cctttcggtt cggtcatagc gatgcgataa gccttgagga
     4561 acgattccgc gacggcgtcg accgaattgg gctggtcgtc gaatttgacg tactcgcgca
     4621 cggcattgcc ttgcaccaaa gcggtgtgca cccagtcggt ggagcgccgg ttcgtggtgt
     4681 cgagcgggcc gccgccgccg aggttgagga taggcgtccg atcgcaccac gcgttgaaaa
     4741 tcgccatgct ggcgtgctgc aagccgacga tatcgtgcaa acccgtcgcc atgatctcgc
     4801 ccgtcgcgcg cgcgtaaccg ttggcgagcg ccactgcgat ctcttcgtgc gtgcacatga
     4861 ttagctcggg ccccttgccg ccttcgaagt tcaccagcga atcgtgcaaa ccgcgatagc
     4921 tcgcgccggg attgagcgcg atatatttaa tccccagctc gcgcagcact tcgaccatca
     4981 gatcggaacc gtactgcgcg ttgctcttgc ggccgatgcc ggctgcccag atttccgcac
     5041 cgccttttgc ttctgccatc tctcctcccg cgttctaatt tcgaatttgc tcgatcttcc
     5101 ggatgaaact gatccaccac aaagatcacg aagccaacga agatcgtaaa caaaccattt
     5161 caaccttcgt gctcttcccg ccttcgtggt gaacgatttt tcctctatcg tcccaaccgt
     5221 tccagagtcg ccacgtaatc gcgcacaccc cgtgctacgt cgtaggcggg ctcgtagccg
     5281 agatttcttt ctgccgccga gatatcgaat acacagtagc tttgtttgct ccgacccagc
     5341 ttgctcggcc cgggcccgag ctcgatctta tgtttcggaa acaatttggc cgcggcatgc
     5401 agaaaatccc tcggcgtcga agccttaccc gtgccgatat tgaaaatcca ttcattcgga
     5461 gcggcggctt tcaaagcgag ataaacggcg cgacccacat cgccgacgta gaccgcatcg
     5521 ttcaactgat ccccgccctg agctatcacc cagctttctc cgctgcgcgc tttctcgatc
     5581 aattgcccat aaaacgacaa cggcccgtgc cgcgcttcct ttcccggccc gtaaatcgac
     5641 acgaagcgca gcgcaatgaa ttcgatgccg taggtttccc ggtaataacg gcccagctcc
     5701 tcgcagcaaa ccttgatcga gccgtacaga tccgccggcc gcttaggata gtcctcgcgc
     5761 acgggcttac atgtgggcgg cgcatactcg ccggtgattt caccataggc cgccttcgag
     5821 ctggtgaaga caatgcgtgt gatccgcttc tggcgcgccg tctccaagac gttgatggtc
     5881 ccgtcgagac cgaccttcac ggcaagacgc ggatcggcct ccgccggttc gggcatcaac
     5941 gctgcgagat gggccatatg cgtgatctga tgacggtcga caatgttttc caatctgccg
     6001 cggtcgcaga tatcgccgat ttcgatatcg acccgatcct tgatgtcggc catcaacgtg
     6061 agatccatcc gattatccat cagcaccggc cgcaggccgt cctgcaccat caagcgcgcg
     6121 gccaccgcgc cgttcacgcc gagaccgccg gtaatcagaa ttcgcatttc aattcctcga
     6181 tgagcatttc tttatccggt ttggcgcgcc tttgtccagc gcaggaagta tagtttccat
     6241 tttccggttt atgcgaaaat cctcggtgcg gatcgtacga aaacttgaag cgaacgtcgg
     6301 gctcattcct tgaagagcaa acgagaaact cgagactaag aacggttcac gctgttgaca
     6361 ccgattcgct tcgcttagta caacgagaac aaagaaggag cacaaaaatg gccgatggaa
     6421 atagctggca accgcctgag tttgtctccc gcgaccgctg catcaaagac accgaagaaa
     6481 ttcttggcat gcctgacctg cccataagaa gcaatgaaga tatttttcgc atcagcaccc
     6541 tcggcatgga ttgg
//
LOCUS       DASULZ010000173         2690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1339965, whole genome shotgun sequence.
ACCESSION   DASULZ010000173 DASULZ010000000
VERSION     DASULZ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339965"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VFY96_03560"
     CDS             complement(<1..495)
                     /locus_tag="VFY96_03560"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605564.1"
                     /translation="MKKASMQLALLAWVFYCLATSAWSQTRIKLNVVYPAVTGVMTAL
                     WTAAEAKAFQKNGLDVSLLYIPSAPQVVRVMLAGDAQITVTGGAAVVSANLSGADFVF
                     IGGIVNVPAFYVMAAPEIKTVQELRGATVGVTRFGSSSDFAMRYVLQKHGLRPDRDVT
                     LLQLG"
     gene            complement(513..1001)
                     /locus_tag="VFY96_03565"
     CDS             complement(513..1001)
                     /locus_tag="VFY96_03565"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5605565.1"
                     /translation="MYPTNEREALEALKGDDPELAATAEALLWSAWCRSGDAEVDRIF
                     RAGVEAMQQQELTEAEGLFTRVIELKPEFAEGWNKRATVRFMRRNFGGSIADCQQTLA
                     RNGNHFGAASGQGLCHMSLSEFREAAVCFRRALEIHPHLDAVRHNLALAESEGGGTGY
                     LN"
     gene            complement(1089..1778)
                     /locus_tag="VFY96_03570"
     CDS             complement(1089..1778)
                     /locus_tag="VFY96_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEX5605566.1"
                     /translation="MGISQGKEVQFFVNEKGGGVSGLLLKPSNASSLLVLGHGAGAGM
                     RHRFMEDVAGKLAERGVATLRYQFPFMEKRTKRPDSEAVLTDTVRAAIGTAKKQAGHL
                     PIFAGGKSMGGRMTSIAAAKEPLDGVLGLVYFGFPLHAAGRPGIERGQHLADLGIPML
                     FLQGSRDALAELKLLKSLCAKLGKRSELFIVDGGDHSFHMLKSSKKSDEQVLDEVVSK
                     AAVWMSTIAGQ"
     gene            complement(2121..>2690)
                     /locus_tag="VFY96_03575"
     CDS             complement(2121..>2690)
                     /locus_tag="VFY96_03575"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605567.1"
                     /translation="AISRFGSLSEFLTRSALKHSGVDPKEVTMLQIGATPARFTTLAS
                     GIVDATILWFPVTERAKAAGMNKLFDLKDLYPNWTNVGFIARESWLAKEKEQTTKFLR
                     AYQRGVRYTRENPDDGIRTLRKYVGLNAAEAAAGYDEYRASFPLDGRILDAGILATVE
                     QEIETGRLKRKIPVSEMIDNSFINAVGKK"
BASE COUNT          590 a          808 c          700 g          592 t
ORIGIN      
        1 gccaagctgg agcagagtca cgtctctatc cggtctgaga ccatgtttct gcaacacgta
       61 acgcatggca aaatccgaag acgatccaaa gcgagtgaca ccgaccgtcg caccccttag
      121 ctcctggacc gtctttatct caggggcggc catgacgtaa aaggccggca cattcacgat
      181 gccaccgata aaaacaaaat ccgccccgct aagattagca ctaaccacgg ccgcgccgcc
      241 ggtcacggtg atctgcgcat ctccggccaa catgactcga accacctgag gggccgacgg
      301 aatatacagg agactaacgt cgagtccgtt tttttggaac gctttcgctt ccgccgccgt
      361 ccacaacgcg gtcatgaccc ccgtgacagc gggataaacc acgttgagtt tgatccgtgt
      421 ttggctccat gcgctggtgg caagacaata gaatacccag gcaagaagcg ccaactgcat
      481 tgaagctttt ttcatagact ctgagaactc gactagttca ggtaaccggt gccgccgcct
      541 tcggattcgg cgagcgcaag attgtgccgg accgcgtcta aatgcggatg aatctccagc
      601 gctcgcctaa agcacaccgc cgcttcgcga aattcgctga gcgacatatg gcagagaccc
      661 tgacccgagg cggcgccgaa atgatttcca ttccgcgcca gggtctgctg gcagtcggca
      721 atcgaaccgc caaagttgcg gcgcatgaag cgcacagtgg cgcgtttatt ccagccttcg
      781 gcgaactcgg gtttgagctc gatgactcgg gtaaacagac cttcggcttc agttagttct
      841 tgttgctgca tcgcttcgac gccggcgcgg aagatccggt ctacctcggc gtctcccgag
      901 cggcaccacg cgctccagag caaagcttcg gcagtggccg cgagctccgg atcgtcgccc
      961 ttcagcgcct cgagtgcttc gcgttcgttg gtcggataca taaaccagtg atcagcaatc
     1021 agtcgtcaca tgtcgcttcg aaaccgcggc ggcccgtcta ggcaagtgtc ggtaccagct
     1081 gagcacgatt actgaccggc gatcgtgctc atccagaccg cagccttact gacgacttcg
     1141 tccagtacct gttcatctga ttttttcgag ctcttcagca tgtgaaacga atgatccccg
     1201 ccatcgacaa taaacaactc ggagcgtttg ccgagtttgg cgcacaagga tttgagcagc
     1261 ttcagctccg ccagagcgtc acgcgaccct tgcagaaaga gcataggaat tccaaggtcc
     1321 gccagatgct ggccgcgttc aatgcccggc cgccccgccg cgtgtagcgg aaacccgaag
     1381 tagaccaggc cgagcactcc atcgagcggt tctttcgctg cggcaatcga tgtcatgcgc
     1441 ccgcccatcg acttgccgcc cgcgaagatc ggtaagtgtc ccgcttgctt tttcgccgtg
     1501 cctattgcgg ctctgacggt gtccgtcaga accgcttcgg agtccggacg tttcgtccgc
     1561 ttctccataa aaggaaactg atagcgcaac gtggcgacac cgcgttcggc gagcttaccg
     1621 gccacgtcct ccataaatcg atgccgcatg cccgcgcccg ctccgtgccc gagcacgagc
     1681 aatgagctcg cgttgctagg ttttagcaaa agccccgaaa ccccgccgcc tttctcattt
     1741 acgaaaaatt gaacttcctt accctgggaa atacccattt cagtctgtct ccaagctctg
     1801 cgttacatcg atcaggtctt tataaaagat aaagttaatc aaataccaga tcatcggcta
     1861 gccaaccact ctatatgcaa tcacagactg tctgctacca gcacgttatt gtcattccgg
     1921 acaagctggt gctagctagc acgatccgca atccagaaag tttctgtgta gtggcgcatg
     1981 gtgtgcgccc tttgagtgcc ggtcccggat cgtccgtaac aagttttcac cggcacgaca
     2041 accaatggcg ctgctgttca aatgaatttg gcttaaacaa gtgcacaaca gactcgctgg
     2101 ccgccaacaa tgagcgcgcg ttattttttt ccgacagcat tgatgaagct gttgtcgatc
     2161 atctcgctaa ctggaatttt gcgcttgagc ctgccggtct cgatctcctg ctcgacggtg
     2221 gccaggatcc ccgcgtcgag aattcgcccg tcgagcggga atgacgcacg atattcgtcg
     2281 tatccggccg ctgcctcggc ggcatttagc ccgacgtatt tcctgagggt tcgaataccg
     2341 tcgtccggat tttcacgcgt gtatcttacg ccccgctggt aagcacgaag aaactttgtt
     2401 gtctgctctt tctctttggc aagccagctc tctcgcgcaa tgaaaccgac gttggtccag
     2461 ttaggataga ggtctttgag gtcaaagagc ttgttcatcc cggcagcttt cgcgcgctca
     2521 gtgacaggaa accataaaat agtcgcatcg acgatgccgc tcgccagcgt ggtgaatcgc
     2581 gccggggtcg caccaatttg caacattgtg acctctttgg gatcgacacc ggagtgcttt
     2641 aacgccgaac gagtgagaaa ttctgacaat gagccgaaac ggctgatcgc
//
LOCUS       DASULZ010000174         2818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_934031, whole genome shotgun sequence.
ACCESSION   DASULZ010000174 DASULZ010000000
VERSION     DASULZ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934031"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VFY96_03580"
     CDS             complement(<1..612)
                     /locus_tag="VFY96_03580"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605568.1"
                     /translation="MKKVLIALHCILLTCCVLASAALAEKLVIAWTAVSAFNAPFWVM
                     NDAGFYKEEGLDVDTIYIASSPIAAKATLAGEIVISAQNSQVIADSGLQGADLVAIGA
                     TVNIVPFYVMAVPEIRSIADLKGKTVGVSRFGAATDFGMRIFLAKHGLTAHRDVAFIQ
                     IGGMPEIATALSKKIIAAAPMSYPMVYVAEQGGAKILANLAKDE"
     gene            complement(643..1638)
                     /locus_tag="VFY96_03585"
     CDS             complement(643..1638)
                     /locus_tag="VFY96_03585"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605569.1"
                     /translation="MKKTIFFALIFTSILLGSRAHGEKFVIAWSAVSALNSPFWVMND
                     AGLLKQEGLDMELVYIASSPTVARATLAGDIVLSGANSQVIVDAGLNGADLVAMGAIT
                     NVVAFYVMAAPEIKTVNDLKGKVVGVTRFGASTDFGMRMLLSKYGLEPSKDVPMIQIG
                     GMPELAAALSKKTVFAAPMSQPMVYLAQQAGMRMIANLAKEEIPFMHIGLTTSKKWIS
                     EHRAQAKAFIRAYGRAIYFMHTRKEETRKIFAKYTKINDPGMLDGSIQYGYDFMEKVP
                     LVKTAAFQVTLDQIAKTNPKAKQAKPEQFFDNSLVQELIDEGFFAKLWGKRGTAE"
     gene            1792..>2818
                     /locus_tag="VFY96_03590"
     CDS             1792..>2818
                     /locus_tag="VFY96_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX5605570.1"
                     /translation="MGLRNQAAIVAIGETPTDRLGSKPGEPRKSSAQYISWAMHLALE
                     DAGLSLKDFQGQGLGVTIPTAYPQPFWPEEVAEILGITPGFLLGGATGGAGAVSLLGG
                     MAAAINSGIIDIALVIGAAAPFSEHFGGGIQPGDMRDWEIPYGTMGPNSKIAMVMRRY
                     MHEYGATLDHFGKIAVAGRHHATLNPLAYLRKPITVQDYKESRLVADPVRLLDCVMPA
                     NGGRAYIVASPERAKSLRKKPIYLKGFGERSNPSYGPRAGSDALVMGVKDAGKVAMQM
                     AGAKHSEINFLELYDDYIIVVYLQIEDLGFCAKGDIGFYERTDFTFKGQLPIQTGGGM
                     INVGQPST"
BASE COUNT          610 a          823 c          754 g          631 t
ORIGIN      
        1 ttcgtccttg gccagattcg ccagtatctt tgcccctcct tgttctgcca cgtagaccat
       61 cggataagac atcggtgcgg cggcgatgat cttcttggag agcgccgtcg caatttccgg
      121 catgccgccg atctgaatga aagcgacatc gcgatgggcg gtaagtccgt gcttggcgag
      181 aaatattcgc atgccgaaat ccgttgccgc gccaaatcga gaaacgccca cagttttgcc
      241 cttgaggtca gcgatgctgc gtatctccgg cacagccatg acataaaaag gaacgatatt
      301 gacggtcgcg ccgatggcga ccagatccgc accctgaaga ccggagtccg cgatgacttg
      361 gctgttctga gcgctgatga cgatctcgcc tgccaacgta gctttggccg cgatgggcga
      421 gctggcgatg taaattgtat cgacgtccaa gccttcttct ttatagaatc ccgcatcgtt
      481 catgacccaa aacggcgcgt tgaacgcgct caccgcggtc cacgcgatga cgagcttttc
      541 cgccagcgcc gctgatgcca agacacaaca tgtgaggagg atgcaatgaa gcgcgatcaa
      601 aactttcttc atggagtcat tactccaaca ctccagcact catcactcgg ctgtacctct
      661 tttgccccaa agtttcgcga agaatccctc gtcgatcagc tcctgcacca agctgttgtc
      721 gaagaattgc tccggctttg cctgtttggc tttaggattc gtcttggcga tttgatctaa
      781 cgtcacctga aaggccgccg ttttcaccag gggaactttt tccatgaagt cgtagccata
      841 ttgaatgctg ccgtcgagca tccccgggtc attgattttg gtatacttgg caaaaatctt
      901 tcgcgtctct tccttgcgcg tgtgcataaa gtagatcgct cggccgtagg cgcgtatgaa
      961 cgccttggcc tgcgccctat gctcgcttat ccatttcttc gaagtggtaa gtccgatatg
     1021 catgaaggga atctcttcct tcgcaagatt ggcgatcatc cgcatcccgg cttgctgcgc
     1081 gaggtaaacc atcggttggg acatcggcgc ggcaaagaca gtttttttgg agagcgccgc
     1141 agcgagctct ggcatgccgc cgatttgaat catgggaaca tcttttgacg gctcgagacc
     1201 gtatttcgag agcagcatgc gcatgccgaa atcggtggac gcgccgaacc gagtcacgcc
     1261 gacgactttc cctttcaagt cgttgacggt tttgatttcc ggcgcggcca tgacgtagaa
     1321 cgcaacgacg ttcgtaatcg cgcccatggc gaccaaatcg gcgccattca accccgcatc
     1381 gacgataact tggctgttcg cgcctgatag gacgatgtcg ccggctaaag tcgcgcgcgc
     1441 caccgtgggg gagctcgcga tataaacgag ctccatgtcc aaaccttcct gctttagcaa
     1501 accggcgtcg ttcatgaccc agaatggcga attcagcgca cttaccgccg accacgcgat
     1561 aacaaacttt tctccatggg cgcggctccc cagcaaaatt gacgtaaaaa taagagcgaa
     1621 aaagattgtc ttcttcatga tgcccactac tccaacactc cgtcactcca atttgattaa
     1681 atacttccag cgacggctag cacactcgta ccggcacgct tcggacgtgt caagtgaacc
     1741 gatttccctt gaattgagcg accgtctgtc ctatggtccg agctgatcgc catgggcctt
     1801 cgcaatcaag ctgcaattgt tgcaatcggt gaaacgccaa ccgaccggct cggatcgaaa
     1861 cccggcgagc cgcgcaaatc gagcgcgcag tatatttcct gggcgatgca cctcgctctt
     1921 gaagacgcgg gtctgagctt gaaagatttt caaggccagg gcctcggtgt cacgatccca
     1981 accgcatatc cacagccgtt ttggcccgaa gaagttgccg agattctcgg catcacgccg
     2041 ggatttctcc tcggcggcgc gactggcggc gccggcgcag tatcgctgct cggcggcatg
     2101 gctgccgcga tcaactcggg gatcattgac atcgcgctgg tgatcggcgc ggcggcgccc
     2161 ttttccgaac acttcggcgg cggcattcag ccgggcgata tgcgcgactg ggaaattccc
     2221 tacggcacca tggggccgaa cagcaaaatc gccatggtga tgcgccgcta catgcacgag
     2281 tacggcgcta cccttgatca tttcggcaag attgccgtcg caggccggca tcatgcgacc
     2341 ttgaatccat tggcctacct gcgcaagccg atcaccgtgc aagactacaa ggaatcgcgc
     2401 ttggtcgcag atccggtgcg gctgctcgat tgtgtcatgc cggccaatgg cggcagggcg
     2461 tacatcgtgg catcgcccga gcgcgccaag tcgctgcgca aaaaaccgat ttacttgaag
     2521 ggcttcggcg aacggagcaa tccttcctat gggccgcgtg ccggttctga cgcgctcgtc
     2581 atgggcgtta aagacgccgg caaggtcgcg atgcaaatgg ctggtgccaa acacagtgaa
     2641 atcaattttc tcgaactata cgacgactat attattgtcg tttacctgca gatcgaagat
     2701 ctcggttttt gcgccaaagg cgatatcggc ttttacgagc gcaccgattt tacattcaaa
     2761 ggacaactgc cgattcaaac cggcggcggt atgatcaacg tcggccaacc ctccaccg
//
LOCUS       DASULZ010000175         3302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_21180, whole genome shotgun sequence.
ACCESSION   DASULZ010000175 DASULZ010000000
VERSION     DASULZ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21180"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1386)
                     /locus_tag="VFY96_03595"
     CDS             complement(46..1386)
                     /locus_tag="VFY96_03595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(325..327),aa:Sec)
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HEX5605571.1"
                     /translation="MIKIVHFVNQFFAGIGGEDKASLAVSTSDSSIGASRALAQKLGD
                     QAQIVRTVYVGDNYFHEHKDEALKEIIEAIRAAKPDVLVAGPAFNSGRYGLACVEICN
                     AVASELGISALTAMHEENPGVGLYREFLNARVFCLPTTESTTGMNDAMARLASFAVRL
                     GRAEELGPASKEGYFGRGIRRLVKADRTGAARAVDMLLKKIAGMPFQTELPMEEWEDA
                     PAAPRLDNIRDSNIAVVTTSGVVPWGNPDKFKTYRNTQWRKYNIAELKELEPGKWEAV
                     HGGYNVAYMNQNPHYGVPLDALRGLESEGAIGPGKLYPAYYVIPGNQGSPAVMRRVGQ
                     EIAADLKKDKVQGVIFVATUGTCSRSGAVMSKELEKAGLPVALISAMFPVAQQVRANR
                     IVKGVSIPHPCGDPNLSKELDARLRREIIRTALKALESEVEAPTVFSPEAAMGG"
     gene            complement(1541..2848)
                     /locus_tag="VFY96_03600"
     CDS             complement(1541..2848)
                     /locus_tag="VFY96_03600"
                     /inference="COORDINATES: protein motif:HMM:NF020896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HEX5605572.1"
                     /translation="MRLEIAEFPVNSIRLGHRFGYADEVLDLDEGALLALLSEDPRIT
                     EVKLAVVHPGEKTRITGIRDIVEPRCKIPGSAEVFPGVLGPVENVGSGRTHRLSRMTV
                     IAAAEYEGTIRAGTTVQRSAILDMSGPGAEVSRFSSYAHLVLSFKILPGLGELDAHSA
                     IQLAEFKIARRLAQITVELSPAKTSIYDLSAKNPELPTVMLVQGCITDPQHVHSGVGY
                     YGLSLRNSMATFTHPNELLDGVVTVDTTRSGRGYYPTTWDWQNHPLVLELYAAHGKRL
                     NFAGVVLQRIRFETSHGKEVGAQNAALLAKAMGADGALVTWIGGGNAFVDVMLTVRAC
                     ERNGIKTALVTYENGGKEGKDSPILFYVGEANGVVSTGALDRPLDLPAVDKVIGPYEQ
                     IKIFPFPGAAPVAARSALSLEARDVMIGGADIWGQGNWQCAEF"
     gene            complement(2947..>3302)
                     /locus_tag="VFY96_03605"
     CDS             complement(2947..>3302)
                     /locus_tag="VFY96_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605573.1"
                     /translation="GEFPIGIAINGETSAAIRDKGAPLGFKVLAPTIVKPEGLFLAKN
                     APHPHAALLFVEWVLSHEAQSFLATTLGKGSAMKGVHSKYKEFALRPDFVVSPKLGAN
                     LQNYIQDFRKVMNAP"
BASE COUNT          686 a         1007 c          861 g          748 t
ORIGIN      
        1 cgactcagcg aacgaaaccg aggcttgttt gaattaagct ggcgctcatc cgcccatggc
       61 ggcctcgggc gagaataccg tcggggcttc aacctcggat tcgagagctt tgagcgcagt
      121 gcgaatgatt tcgcggcgta aacgcgcatc gagctctttc gagagattgg gatcgccgca
      181 gggatgcgga atgctcacgc ctttcacgat ccgattcgca cgtacctgct gcgccaccgg
      241 aaacatcgcg ctgatcaacg ccacgggtag ccccgctttc tcgagttctt tgctcatcac
      301 agcgccgctg cgactgcagg ttcctcacgt ggcgacgaag atcactcctt ggactttgtc
      361 cttctttaga tccgccgcga tctcttgacc gacgcggcgc atcaccgccg gtgatccctg
      421 atttcccggg atcacatagt acgcgggata aagctttccg ggcccaatcg ctccctccga
      481 ttccaacccg cgcaaggcat cgagcggcac gccgtagtgt gggttctgat tcatataagc
      541 gacattgtag ccgccatgca ccgcttccca cttgcccggc tcgagctctt tcaactcagc
      601 gatattgtat ttgcgccact gtgtgttgcg gtaggtttta aatttgtccg gattgcccca
      661 gggaaccact ccgctcgtgg tcaccaccgc aatattgctg tctctgatgt tgtcaagccg
      721 cggcgccgca ggcgcatctt cccattcctc catgggaagc tcagtctgaa aaggcatacc
      781 cgcgattttt tttagcagca tatcgaccgc gcgtgccgcg ccggtcctat ccgccttcac
      841 cagccgtcga attccacggc cgaaatatcc ttctttcgac gccggaccga gctcttccgc
      901 acgcccgagt cggaccgcga agctggccag ccgcgccatc gcatcattca tgccggtggt
      961 tgactccgtt gtcggtaaac aaaaaacccg cgcattgaga aattctctgt agagcccgac
     1021 gccgggattc tcctcgtgca ttgcggtcaa tgccgaaatg ccgagctcac tagcgacagc
     1081 gttgcagatc tcgacgcaag ccaaaccgta tcggccggaa ttgaacgccg gacccgcaac
     1141 caaaacgtcc ggtttcgcgg cgcgtatcgc ctcaatgatc tccttcagcg cctcatcttt
     1201 gtgctcgtgg aagtaattat cgccgacgta aactgtgcgt acgatttgcg cttgatcgcc
     1261 aagtttttgc gccaaagctc ggctcgctcc aatgctgctg tcactggtcg aaaccgccaa
     1321 gcttgccttg tcttctccac cgatcccggc aaaaaactga ttcacgaaat gaacgatttt
     1381 aatcatggcg gcttctccta aaactccacg atgcttcccg aacaatatta tttgaaacaa
     1441 gaaagtacat aactgtcatg ctgagcggta gcgaagcatc taggtttttc agtacttgac
     1501 cacggcctaa ttcggcgcag taaagcgtac cgtctcatga tcagaattcc gcgcattgcc
     1561 aatttccctg tccccaaatg tccgctccgc cgatcatgac atctctcgcc tctaaagata
     1621 aagcactacg tgcggccaca ggagcggcgc ccggaaaagg gaaaattttg atctgttcgt
     1681 atggaccgat cactttatcc accgcgggga ggtccaaagg ccgatcgagc gcgcccgtgc
     1741 tgaccactcc gttggcttca ccgacataga agaggatcgg gctgtctttg ccttccttac
     1801 cgccgttctc gtatgtcacc agcgccgttt tgatgccgtt gcgctcgcaa gcgcgcacag
     1861 tcaacatcac atcgacgaac gcgttaccac caccgatcca ggtgacgagt gcgccgtcgg
     1921 cgcccatggc tttggcaaga agcgcagcgt tctgtgcacc gacctcttta ccgtgactgg
     1981 tttcgaagcg aatgcgttgc aacaccacac cggcaaaatt caaccgtttc ccgtgggcag
     2041 catacagttc caacaccaaa gggtgattct gccagtccca agtcgtcgga taatagccgc
     2101 gcccggaccg ggtcgtatcg accgtcacca cgccatcgag aagctcgttc gggtgagtga
     2161 atgtggccat ggagttgcgc aacgaaagtc cgtagtaacc tacgcctgag tgaacgtgct
     2221 gcgggtccgt gatgcatcct tgtacaagca tgacggtcgg aagctcggga tttttcgcgc
     2281 tgagatcata aattgaagtc tttgccgggc tcagctcaac cgtgatctgc gcgagccgcc
     2341 gcgcgatttt gaactccgcc agttgaattg cgctgtgggc gtcgagttca ccgagacccg
     2401 gcaagatttt gaagctcaat accaaatggg cgtacgagct gaagcgggat acttccgcac
     2461 cgggtccgga catatccagg atcgcgctcc tttgtactgt cgttcccgct cggattgtgc
     2521 cttcgtactc cgctgcggca atgacggtca ttcgtgaaag ccggtgcgtt cggccagagc
     2581 cgacgttctc aaccggcccc aggacccccg gaaaaacctc ggcgctcccc ggaatcttgc
     2641 atcgtggttc gacgatatcg cgtattccgg taatgcgtgt cttttcgccg gggtgtacaa
     2701 ccgccagctt tacttcagtg atcctcgggt cttccgaaag cagcgcaagc agagcgcctt
     2761 catcgaggtc gagtacctca tcggcgtagc cgaaccgatg ccccagacga atcgagttta
     2821 ccggaaattc tgcgatctca agtctcatgg cggtctctct cgtccccctc cttcgtcctc
     2881 cccccgacgc gggggaggaa agaggtgggg atcgaacggt ttgaacgatt tgaacaacca
     2941 acttcttcac ggcgcattca tcactttccg aaaatcctga atgtaatttt gcaagttcgc
     3001 gccgagtttc gggctcacca caaaatcggg ccttagggca aactctttgt acttgctatg
     3061 cacgcccttc attgcgcttc ctttgcccag ggtcgtggcg aggaaagatt gcgcttcgtg
     3121 cgagagcacc cactcgacaa atagtaaagc cgcatgcgga tggggcgcat tctttgccag
     3181 aaacagacct tcgggcttga cgatcgtcgg cgcgagaact ttgaatccca gcggcgcacc
     3241 tttgtcgcgg atcgccgccg aggtctcgcc gttgatggca atcccgatgg ggaattcacc
     3301 cg
//
LOCUS       DASULZ010000176         3429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_934108, whole genome shotgun sequence.
ACCESSION   DASULZ010000176 DASULZ010000000
VERSION     DASULZ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934108"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VFY96_03610"
     CDS             <1..743
                     /locus_tag="VFY96_03610"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682001.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX5605574.1"
                     /translation="LVIYDDLSKHAVAYRQLSLLLRRPPGREAYPGDVFYLHSRLLER
                     AAKMSDQRGGGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLESDLFYSGVRPAIN
                     VGISVSRVGGNAQIKAMKQVAGTLRLELAQYREMAAFAQFGSDLDQATQRQLNRGSRL
                     VELLKQGQYQPLPVEKQILIIFAGTNGFVDELPLTALKKYEQELYSFVDSRHPDVFAD
                     ILKKRELDGDLRGKLTKTLEEFKGVFKP"
     gene            826..1692
                     /gene="atpG"
                     /locus_tag="VFY96_03615"
     CDS             826..1692
                     /gene="atpG"
                     /locus_tag="VFY96_03615"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEX5605575.1"
                     /translation="MATLKAIRKRISSVRNTQQITKAMKMVSAAKLRRAQEAAVAARP
                     YAEKMTELLKNVAARVSIEAHPLLQTREEKKIDLVLFTSDRGLCGGYNANLIRAAEAF
                     IRRHAPDKEVELTLVGRKGADHFRRRRAPIADRYLNVLTTVPDELAALIGQKLISRFI
                     NRETDAVYILYSHFRSALSQVPTLEKLLPVSLTETDHTEAQQLTEYLYEPGIEQLLAS
                     LLPRITDVAVQRALLEATASEHGARMTAMDSATSNASKMIGSLTLQMNRARQASITRE
                     LMEIVGTAEALK"
     gene            1776..3185
                     /gene="atpD"
                     /locus_tag="VFY96_03620"
     CDS             1776..3185
                     /gene="atpD"
                     /locus_tag="VFY96_03620"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX5605576.1"
                     /translation="MGMGKITQVLGAVVDVEFSDSSLPPIYNALKVSNPAISDQQWNL
                     IVEVAQHLGENTVRCIAMDTTEGLVRGMDVMDTGDGITVPVGEETLGRVLNVIGEPVD
                     EMGPVNAKKSYPIHRPTPEFVDQETKVQSFETGIKVVDLLAPYARGGKIGLFGGAGVG
                     KTVILMELINNVAMKHGGYSVFGGVGERTREGNDLWLEMKESGVINKASLVYGQMNEP
                     PGARARVALTALTLAEFFRDDEGRDVLLFLDNIFRFTQANSEVSTLLGRMPSAVGYQP
                     TLATDLGELQERITTTHKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRQIAE
                     LGIYPAVDPLDSTSRILDPLVVGTEHYDVARQVQVILQRYKDLQDIIAILGMDELSEE
                     DRLTVARARKIQRFLSQPFHVAEAFTGTPGAYVELKDTIRGFKEVVDGKHDDIPEQAF
                     YMVGTIEQAVEKAKKMAAA"
BASE COUNT          757 a          995 c          989 g          688 t
ORIGIN      
        1 ccctggtcat ttacgacgat ctatcgaaac acgcagtcgc ctatcgccag ctatcgctgc
       61 tgctgcgccg cccaccggga cgcgaagctt atccgggcga tgttttttat ttgcattccc
      121 gtctgctcga gcgcgcggcg aagatgagtg atcagcgcgg gggaggttcg ctgaccgccc
      181 tgccgatcat tgaaacccag gccggggatg tttccgccta tattcccact aacgtcattt
      241 cgatcaccga tgggcagatt tttctggaga gcgatctgtt ctattccggc gtccgccccg
      301 cgatcaacgt cggcatttcc gtctcgcggg tcggcggcaa cgcgcagatc aaagcaatga
      361 aacaggtcgc cggcacgctg cgcttggagc tcgcgcagta tcgcgaaatg gcggccttcg
      421 ctcagttcgg ctccgatctc gaccaagcca ctcagcgcca gcttaaccgt ggatcacgat
      481 tagtcgaatt gctcaaacag ggacaatatc agccgctgcc ggtcgagaaa cagatcctga
      541 tcatctttgc cgggaccaac ggcttcgtcg atgagctgcc gctcacagcc ttgaagaaat
      601 acgagcagga gctctactcc ttcgtcgact cgaggcatcc ggatgtgttc gccgacattc
      661 taaagaaacg cgaactggac ggcgatctgc gtgggaaatt gaccaagacg ctggaagaat
      721 tcaaaggggt ctttaaaccc tagtttcagg actgagggcc gaggtatgag gactgagaaa
      781 agaggaactc aagcctcagt cctggtgcct cagtcataac aagtcatggc cacgctcaaa
      841 gcgatacgta agcggatcag ctcagtccgc aacactcagc agatcaccaa agccatgaag
      901 atggtttcgg ctgccaagtt gcgtcgcgcc caagaagcgg cggtagcggc acgcccgtac
      961 gctgaaaaga tgaccgagct tttgaaaaac gtcgccgcca gagtttccat cgaagctcat
     1021 ccgctgctgc aaacgcgcga agaaaaaaag atcgatctcg tgctgtttac ttcagaccgg
     1081 ggcctctgcg gcgggtacaa cgcgaacttg attcgcgctg cggaagcgtt tattcggcgc
     1141 cacgctcccg ataaagaagt tgagctcacc ctagtcggcc gcaagggcgc cgatcatttt
     1201 cgacgccggc gcgcgcccat cgctgatcgt tatctgaacg tcctgacgac agtgccggac
     1261 gagctagcag cactgattgg tcagaagctg atctcacgtt ttatcaatcg cgagaccgac
     1321 gcggtataca ttctttacag ccattttcgc tctgcgcttt cgcaggtgcc gacgctcgag
     1381 aagcttttgc ctgtgtcatt gaccgagacc gaccacaccg aggcgcaaca gctcacggaa
     1441 tatctatacg agccgggcat cgagcaactg ctggcgagtt tgctgccgcg catcaccgat
     1501 gtcgccgtgc agcgcgcgct gctcgaggcc acggcgagcg aacatggcgc gcgcatgacc
     1561 gccatggact ccgccaccag caatgcttcg aagatgatcg gcagcctgac cttgcagatg
     1621 aaccgagcgc gccaggcgtc gatcacccgc gagctgatgg aaattgtcgg cactgcggaa
     1681 gcattgaagt aatccccctt cttcgtcctc cgccgcagcg cggggaggaa agatgtgggg
     1741 cggaacacga acacgttttt ggaggtttct acattatggg catggggaaa atcacccagg
     1801 ttcttggcgc cgtcgtcgac gttgagtttt ccgactcctc gcttcctccc atttataacg
     1861 ccttgaaagt ttccaacccg gcgatcagcg atcagcaatg gaatttgatc gtagaagtgg
     1921 cacagcacct gggtgaaaac acggtgcgat gcatcgcaat ggacacgacc gaggggttgg
     1981 tacgcggcat ggacgtcatg gacactggcg acgggatcac cgtacccgtt ggcgaagaga
     2041 ccctcggacg ggtgctcaac gtgatcggtg aaccggtaga cgagatgggg ccggtcaacg
     2101 ccaaaaaaag ttacccgatt caccgcccaa cgccggaatt cgtcgaccag gaaaccaaag
     2161 ttcaatcgtt cgagaccggc atcaaagttg tcgacttgct cgccccctat gcccgcggcg
     2221 gcaagatcgg cttgttcggc ggcgcgggcg ttggcaagac ggttattctc atggagttga
     2281 tcaacaacgt cgccatgaaa catggcggct attcggtctt cggcggcgtc ggcgaacgca
     2341 cccgcgaagg caacgatctt tggctcgaaa tgaaagagtc gggcgttatc aacaaggcct
     2401 cgctcgtcta cggccagatg aacgaaccgc ccggagcgcg cgctcgcgtc gccttgaccg
     2461 ccctcacact ggcggaattt ttccgcgacg atgagggccg ggatgtgctc ttgtttctcg
     2521 ataacatttt ccgtttcacc caggccaact ccgaggtttc gaccctgctc ggccggatgc
     2581 cttccgcagt cggttatcag ccgacgttgg ccacagactt gggcgagctt caggaacgaa
     2641 tcacgaccac gcacaagggc tcgattacat ccgtccaggc gatctatgtt ccggcggacg
     2701 acttgaccga tccggcgccg gcgaccacct tcgcgcattt ggatgccacc acggttctct
     2761 cgcgccagat cgccgagttg ggaatctatc ctgcggtgga tccgctcgat tcgacttcgc
     2821 gcattcttga cccgctcgtc gtgggcacgg aacattacga tgtggcgcgc caagtccagg
     2881 tgatcttaca gcgctacaaa gacctgcagg acatcattgc cattctgggc atggacgaat
     2941 tatcggagga agaccgcctg accgtggcgc gggcgcggaa aattcagcgt ttcctgtcgc
     3001 agccatttca tgtcgcggaa gcgtttaccg gcactccggg cgcctatgtg gaattgaagg
     3061 ataccatccg cggcttcaaa gaggtggtcg acggcaagca cgacgatata ccggagcagg
     3121 ctttttacat ggttggcacc atcgagcagg cggtcgaaaa agccaaaaag atggcagcag
     3181 cctaggaggc gcgaggcgtg aggccgaatt cgtactcatg ccgcagccct caagcctcat
     3241 tcccgtaaat catgggagac aaaatcagac tgagagtggt gacgccgagc cggttgcttc
     3301 tcgacgagga tgtcgatgaa gtgacggccc atggagaatt gggtgagttt ggcgtcctgc
     3361 ccaatcacat cgcattctta tcgaccctcg tggccgggga gctgagttac aaacagggcg
     3421 cggcacggc
//
LOCUS       DASULZ010000177         2561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1137685, whole genome shotgun sequence.
ACCESSION   DASULZ010000177 DASULZ010000000
VERSION     DASULZ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137685"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..1435)
                     /locus_tag="VFY96_03625"
     CDS             complement(17..1435)
                     /locus_tag="VFY96_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005368925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX5605577.1"
                     /translation="MKPTRWTLVGNWILERFPLSKLWNEHMAHYYAPKNFNFWYFFGS
                     LALLVLVNQIITGILLTMNYKPDAELAFDSVEYIMREVNWGWLIRYMHSTGASAFFIV
                     VYLHMFRGLMYGSFKHPRELIWIFGMCIFVALMAEAFMGYLLPWGQMSYWGAQVIISL
                     FSAIPVIGEDLSLWIRGDYVISDATLNRFFAFHVIAVPLVLVMLVFLHIVALHEVGSN
                     NPDGVEIKKLKDAKGIPLDGIPFHPYYTVKDIVGVAVFLIFFSVVIFYMPELNGFFLE
                     HANFIPADPMKTPEHIAPVWYFTPFYSVLRAIPDKFAGVIGMGGAIVVLFFLPWLDRG
                     KVKSVRYRGGIYKKALFLFVVSFLTLGYLGTQPPTPAASVIARICTAIYFGFFLLMPV
                     YTRWEKLKPVPTRVTQQPSFDDRLRAAGKLYEEMTYALPPSSEQPASPLAKCAFLRRR
                     LNPAGIRSVAKRFVKTLKTSLD"
     gene            complement(1432..2031)
                     /gene="petA"
                     /locus_tag="VFY96_03630"
     CDS             complement(1432..2031)
                     /gene="petA"
                     /locus_tag="VFY96_03630"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005368923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEX5605578.1"
                     /translation="MNNQGVDKSKRQFLTSALTVVGAVGTGYLAVPFLAQMQPSVKAM
                     AAGAPVEVDISKMEPGQLLRVAWRGKPVWILKRTPEVLATLKTLDNELRDPLSLESIQ
                     PEYSKNLYRSVKPEIFVAIGICTHLGCSPTFRPELAPADLGDKWKGGFFCPCHGSWFD
                     LAGRVYRGVPAPTNLEIPPYRYVTENQLIIGESSQGASA"
     gene            complement(2220..>2561)
                     /locus_tag="VFY96_03635"
     CDS             complement(2220..>2561)
                     /locus_tag="VFY96_03635"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005368921.1"
                     /note="catalyzes the formation of L-histidinol phosphate
                     from imidazole-acetol phosphate and glutamate in histidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase"
                     /protein_id="HEX5605579.1"
                     /translation="VLTQASAEFALSHKALFDQQTRLIRAERARVFAALETIEGITAY
                     PSAANFILFRTPPDHANAVFAGLKQRGVLIKNLSPQGGILADCLRVTVGKPDENEAFL
                     FALNQSLISNN"
BASE COUNT          633 a          693 c          705 g          530 t
ORIGIN      
        1 tattattcta ttcatatcaa tcgaggctcg ttttgagggt tttgacaaag cgcttggcga
       61 cactgcgaat gccggccggg ttcagccttc ttctcaaaaa ggcgcatttg gcaagcggcg
      121 atgcgggctg ctcggaggac ggcggcaatg cataagtcat ttcttcatag agctttccgg
      181 ctgcacgcag acggtcgtcg aagctcggct gctgagtcac ccgggtcggt accggtttca
      241 gtttttccca gcgcgtatac accggcatca gcaggaagaa accgaaatat attgctgtgc
      301 agattctcgc aatgacgctt gcggccggag tcggcggctg cgtacccagg tagccgagag
      361 tcagaaagct gaccacgaac agaaacagcg cttttttata aatgcctccc cggtaacgaa
      421 ccgatttgac tttgccccga tcaagccagg gcaggaaaaa cagcacgacg atcgcgccgc
      481 ccatgccgat caccccggca aatttatccg gaatcgcgcg taggaccgaa tagaacgggg
      541 tgaaatacca caccggcgcg atatgctccg gcgtcttcat cgggtcggcc ggaatgaaat
      601 tggcgtgttc caggaagaag ccgttcaatt ccggcatata gaagatcacg accgaaaaaa
      661 agatcagaaa taccgcgaca ccgacgatat ccttgaccgt atagtaagga tggaagggta
      721 tgccgtcgag cggaatgcct ttggcgtcct tcagtttttt aatctcgacg ccgtcgggat
      781 tgttggagcc gacttcatgc aatgcgacga tatgcagaaa caccagcatc accagcacca
      841 gcggcaccgc gatcacatgg aacgcgaaga agcggttcag cgtggcgtcg gagatcacgt
      901 aatcgccgcg aatccacaat gacaagtctt cgccaatcac cggaattgcg ctgaacaacg
      961 aaatgatgac ctgcgcgccc cagtaagaca tttgccccca aggcaacaga tagcccatga
     1021 aggcttcggc catcaacgcg acgaagatgc acatgccgaa aatccagatc agctcgcgcg
     1081 gatgcttgaa cgagccgtac atcaggccgc ggaacatgtg cagataaacc acgatgaaaa
     1141 acgcggaggc gccggtcgag tgcatatagc ggatcagcca gccccagttg acctcgcgca
     1201 tgatgtattc gaccgaatcg aacgccagct ccgcgtccgg tttatagttc atggtcagca
     1261 ggatgccggt aatgatctgg ttgaccagca ccaagagtgc cagcgacccg aaaaaatacc
     1321 agaaattgaa atttttcggc gcatagtaat gcgccatatg ttcgttccac aacttggaga
     1381 gcggaaaacg ctcgaggatc cagttgccga ctaatgtcca acgagtaggt ttcatgcgct
     1441 tgccccttgg ctggattcgc cgatgatcaa ttggttttcg gtcacataac ggtaaggagg
     1501 aatttccaga ttggtcggcg caggcacacc gcgataaacg cggccggcca gatcaaacca
     1561 ggaaccgtga cagggacaga agaaaccgcc tttccattta tcgccgaggt cggcgggcgc
     1621 aagctcaggt ctgaaggtgg gcgaacagcc caggtgagta cagatgccga tcgccacgaa
     1681 aatttcgggc ttgacggagc gatagagatt tttgctgtat tcgggttgaa tcgactccag
     1741 ggataaagga tcgcgcagtt cgttatccag cgttttcagc gtcgccagca cctcgggcgt
     1801 gcgcttcaaa atccacaccg gtttgccgcg ccaggcgacg cggagaagct ggcccggctc
     1861 cattttgctg atatcaacct cgaccggagc cccggccgcc attgccttca cgctcggttg
     1921 catctgagcg agaaagggta cagctaaata acctgtaccg accgccccca ctacggtcag
     1981 tgccgaggtt aaaaactggc gtttagactt gtcgacgcct tggttattca ttatctaact
     2041 ctcctgttgc aggtctatgc cgtaataaac gcattatcgg gcgcaatata ccactagaac
     2101 ctagtcgatt tgaagtgctt aatcgatatt gtttaccggg agatgacaaa aacgcggcga
     2161 tgaaaaggcg aaaaccggag cgtttagctc tctcggtctg cctcccatcg ccgaaagact
     2221 caattattgg aaataaggct ttgatttagt gcgaaaagaa acgcttcgtt ctcatcgggt
     2281 ttgccgaccg tcacgcgcag gcaatcggcc aaaatgccgc cctgggggct caaattcttg
     2341 atcaggacgc cccgctgttt aagtccggcg aataccgcgt tcgcatggtc cggcggcgtt
     2401 ctgaacagga tgaaattggc cgcgctcggg taagcggtga tgccttcgat cgtctccagc
     2461 gcggcgaaga cgcgcgcgcg ttcggcgcgg atcaagcggg tttgctggtc gaacagcgct
     2521 ttatgactca acgcgaattc cgcactcgcc tgggtcagca c
//
LOCUS       DASULZ010000178         2706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_21607, whole genome shotgun sequence.
ACCESSION   DASULZ010000178 DASULZ010000000
VERSION     DASULZ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21607"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            361..567
                     /locus_tag="VFY96_03640"
     CDS             361..567
                     /locus_tag="VFY96_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605580.1"
                     /translation="MHSLRPSTWFLAHSRFIDTYETLHPGAAGFTWSNRNFFAQNHRP
                     VLPDQRIDYILTNHSFAGEPSRAM"
     gene            complement(682..894)
                     /locus_tag="VFY96_03645"
     CDS             complement(682..894)
                     /locus_tag="VFY96_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605581.1"
                     /translation="MIDVSLKGIRCRAAAEILDVDGAISAATEGTIIYELEGEGGQLV
                     NVHWDDGTRSLVSSEEIVITDAVTWQ"
     gene            complement(1116..2447)
                     /locus_tag="VFY96_03650"
     CDS             complement(1116..2447)
                     /locus_tag="VFY96_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX5605582.1"
                     /translation="MPSLIDLRAHAREIFFAGLSAADPLDAINHAVQRESHRLRVGKR
                     FYDLDRFQRIYITGCGKAAARMALAMERLLGDRIAGGIVVAKYGHGVKLKGTEVIEAG
                     HPVPDDAGLNGARRIAELLQSCDKNDLVFFLLSGGGSALLPYPAEGLSLTDKQRTTEA
                     LLKSGARIREINAVRKHLSRLKGGRLADLAAPATLICLVLSDVIGDSLETIASGPTVP
                     DSSTYSDCLDIIRRYQLGPKVPSAVLEYLERGARGQVAETPKRLSGIFDGVQNVIIGN
                     IRTALSSALRRAEELGYHTTIFSEAIQGESRAVARRFAALLKEIAAKDQPVSRPACVI
                     SGGETTVTIRGDGLGGRSQEFALAAALEISGVEQIVVLSAGTDGTDGPTDAAGGIVDG
                     KTVERAVTQGLDPAAFLDRNDSHHLLQATHDLLFTGPTLTNVMDLQVGLVA"
BASE COUNT          582 a          777 c          735 g          612 t
ORIGIN      
        1 attttgcttt ttcagcagct tttcgacgca gactggagca aggagttgca ggcgcgcttc
       61 ggccatcctt attggtttca accgagtgca ggcattccag tctagtggtc ttgagcaagc
      121 tgccgccgga cggcaatgaa atatatcgga tgaacaccaa atcgcccttt gaaaattatt
      181 ggcgtgatgc gctctgggcc ggactcagcg ctggcggtca ccgctaaatg tattcaacat
      241 ccatctgtcc tgggaacttg aggatcattc aacccgggag gcgcagctgc gcgagctttg
      301 gtcgttcatt gagacgcgtc gatctgagaa cggtttcgcc atcattgccg gagattttaa
      361 ttgcactctc tccggccatc gacttggttt cttgcccatt cgcgcttcat cgatacctat
      421 gaaactctgc atcccggcgc agcgggattt acctggagca acagaaattt ctttgcgcaa
      481 aatcatcgcc cggttctccc cgaccagcgc attgattaca tcctgacaaa ccattcgttc
      541 gcgggagaac cgtctcgggc gatgtgaagt gattttcgat acggccgcaa cgctgggcgt
      601 atttgcctcc gatcactttg gccttctggc ggagtttgga ctatctcgcg gtgcggattt
      661 gtagtcttgg gtcaatgata attactgcca tgtgacagcg tcggtgatga caatttcctc
      721 ggacgaaacc agcgagcgcg tgccgtcgtc ccagtggacg ttcacgagtt gtccaccttc
      781 gccttcgagc tcgtaaataa tggttccctc cgtcgctgcg ctaatggcgc cgtcgacgtc
      841 caggatttcc gcagccgcac ggcaacgaat ccctttcaga ctcacgtcga tcatttttgc
      901 tccaggacgt gttacttgtg atcaatgtgc ttcgaatcgg agaagcaaac tctaaccacg
      961 gatgaatgtt gatttcaacg gttcagcgac ggcgctagcg acgtttgcag ggagcttctt
     1021 tcttatccag ttccgagcga cagaaaaccc gcctagcggc gaccagacca gagcctcatt
     1081 gttgagtatg aagcaccgtt cgagcggcag tcctctcagg cgaccaaccc gacctgtaaa
     1141 tccatgacgt tggtcaacgt cgggccggta aataaaagat cgtgggtagc ttggagcaga
     1201 tgatgggaat cgtttcggtc gagaaaggct gcagggtcca aaccttgagt gacggcacgt
     1261 tcgacagttt tcccatccac aatgccgccg gctgcatcgg tcggtccatc tgtgccatca
     1321 gtgccagcgc tcagtacgac aatttgctcg acgccgctga tttcaagtgc cgcagctaat
     1381 gcaaattcct gactccgccc gccgagtccg tcccctcgaa ttgtcaccgt cgtctcaccg
     1441 cctgagataa cgcaagcggg ccgagaaact ggctggtctt tggcggcgat ctctttgaga
     1501 agcgcagcaa atcttcgagc gacagcgcgg ctttcacctt ggatcgcttc ggagaaaatc
     1561 gtcgtatggt aacccagctc ttcggctcgc cgaagggccg agctgagcgc cgtccgaatg
     1621 ttgccgataa tcacattctg cacgccgtca aaaatgccgc tgagacgctt cggtgtttct
     1681 gcgacctgtc ctcgggcgcc gcgctctaaa tattccagca ctgcagacgg aactttcgga
     1741 ccaagttgat agcggcgaat gatgtcaagg caatcgctat aggtgctgct gtccgggacc
     1801 gtcggaccgg aagcgatcgt ttccagtgaa tcgccgatga cgtccgaaag caccaggcaa
     1861 atcagcgtcg ctggcgccgc aagatcggct agccggccac ccttcaggcg cgacagatgc
     1921 ttacgtacgg cattgatctc gcgtatgcgc gcgccgctct tcagaagcgc ttcggtggtc
     1981 cgttgcttgt cagtcagtga caaaccttct gccggatacg ggagtaatgc gctgccgcca
     2041 cccgagagca gaaaaaaaac caaatcgttc ttatcgcatg actgcagcag ttcggcgatt
     2101 cgccgcgctc cgttcagccc ggcatcgtct ggcacgggat ggcccgcctc aattacttcc
     2161 gtgcctttca gttttacccc gtggccgtat ttggccacga caattcctcc ggcaattctg
     2221 tcgccgagca ggcgttccat agcgagtgcc atccgggccg ccgctttgcc gcagcccgtg
     2281 atgtagatcc gctgaaaccg atcgagatcg taaaagcgtt tgccgacccg aagccggtga
     2341 gattcgcgct gaaccgcatg attgatggcg tcgagtggat cagcagccga caggcctgcg
     2401 aagaatattt cccgcgcatg cgcgcgcaga tcaatcagcg acggcatcag cccgcgcctc
     2461 attgagaatc aatcggttaa gcggcacaga tggcgatggt aggtttcaaa aaaacgcgtg
     2521 acgagcgaac taagggcgtg ggtcgaacaa acggaagccg ccgaacccat actcaccaat
     2581 cggcttcccg ctgttcgcat cgaagatgtc gatgagcata ttcttctccg gatggccggc
     2641 gagaaagcgc gaaaaaatct cgacgaactg ttccttctcg gttgcactgg cgcgaacgaa
     2701 accatc
//
LOCUS       DASULZ010000179         4942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_630010, whole genome shotgun sequence.
ACCESSION   DASULZ010000179 DASULZ010000000
VERSION     DASULZ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630010"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="VFY96_03655"
     CDS             <1..913
                     /locus_tag="VFY96_03655"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5605583.1"
                     /translation="GVGFAALTMTSQATFLSNWFVRKRGMAIGVAASGIGFGILLVVP
                     WTQWLIAVYGWRSALFVLAGVLALIVAPANFLFQRQRPEEMNLKPDFGQPGKISVHEV
                     KAKTASGPTLRDALRDWRFWAFAAGVLAGAIPLHMVLIHQVAAVSDAGFSKESAAWAL
                     GLIGLFTAPAMIGMGLLADRIGRQWAYALGSGAFMIGIFFLMTIDAVSGSWLFYAFPP
                     FIAVGFSSRQSLYPTIAADLFHGKSFGAIIGALALFIGAGAGLGPWLGGLIFDWTGGY
                     YWAFCIAQGMTVASVVFIWLAGSSRTH"
     gene            1043..1465
                     /locus_tag="VFY96_03660"
     CDS             1043..1465
                     /locus_tag="VFY96_03660"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5605584.1"
                     /translation="MEWKEALPLLRENHTAVAISVTPKGHAQSTIVSTALLDDKLGFA
                     SRPRTVKLKNIQRTGRAAITVIKLDNRRYVTVEGPASIEPWQNTPAHIKRLKDFYASM
                     GRAQKGTDEDFARQMRAEERSLILITPEHVYGSLQSGA"
     gene            complement(1604..3322)
                     /gene="ybaL"
                     /locus_tag="VFY96_03665"
     CDS             complement(1604..3322)
                     /gene="ybaL"
                     /locus_tag="VFY96_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HEX5605585.1"
                     /translation="MPHNVSLITTIAAALGLALAMGFVAIRLKLPPLVGYLIAGIIIG
                     PATPGFVADVGLSQQLAEIGVMLLMFGVGLHFSFDEILAVRRIALPGAIVQIAAATAL
                     GAAVAHSWGWNIAASISFGLALSVASTVVLLRALEDRDILATLNGRIAVGWLIVEDLA
                     MVLVLVLLPSIGAWNGIHGPGTTAESIDRSLSATLGLALARVAVFIGLMLLVGRRFFP
                     WLLWQIARVGSRELFTLCIIAAAVGIAYGSAALFGVSFALGAFFAGMVMRESPLSYRA
                     AEESLPFRDAFSVLFFVSVGMLFEPRVLIREPLHVLIVLAIIIVGKSLAAFFLVLLFR
                     YPLNTALIVSASLAQIGEFSFILAGLAVSLKLMPAEGQSLVLAGAIISIGLNPLVFRA
                     IEPVQNWIRSRSKLAQALERPTDPLAALPTSIDLKRLTGQVVLVGYGRVGSRIGAALT
                     AQGIPFVVAEENREIVEKLRERDIPAVSGDASEPAVLIQAHIHRARMLVTAIPDIVQV
                     RRIIETARTVNPRVEIVVRTHSEKESALLEKEKIGAIFFGEQELARSMTQHVIDRYAA
                     RTATHF"
     gene            3468..4550
                     /locus_tag="VFY96_03670"
     CDS             3468..4550
                     /locus_tag="VFY96_03670"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX5605586.1"
                     /translation="MRLPVNPPVLPMLAKRVSELPRAGAWIFEPKWDGFRALVFRDAD
                     EIHIQSRDEKSFNRYFPELLAPLCSQLPARCVLDGEIVIAQNGALDFDALQLRIHPAQ
                     SRVKLLAKETPASIVFFDALSEGDRDLRGAAFEERRSILERLLSSAKAPIHLTPATRE
                     TKVAADWFRRFEGAGLDGVVAKPVRGIYEPNKRAMLKIKHERECDCVVAGFRWHKKGE
                     RTMLGSLLLGLYDDSGELNHVGVCASFTEKKRRELVEFLAPYRKNALVHHPWKNWAEQ
                     ANGEADYRMPGGQSRWSSGKDLSWEPLRPELVVEVAYDHMQGDRFRHTAQFRRWRIDK
                     KPKDCTYDQLEVVAPEELATIFASGR"
     gene            4736..>4942
                     /locus_tag="VFY96_03675"
     CDS             4736..>4942
                     /locus_tag="VFY96_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605587.1"
                     /translation="MKKVTFVSLILILFTAGWCAGAERMRIGYSSISGSYLGIWVAHD
                     AGYFAKEKLEDQIILIPSGSQLAQV"
BASE COUNT         1117 a         1348 c         1458 g         1019 t
ORIGIN      
        1 gggcgttggt tttgcggctc tgacgatgac gtctcaggcg acctttctca gcaactggtt
       61 tgttcggaag cggggcatgg cgatcggtgt tgctgcctcc ggcatcggat tcggcattct
      121 tctggtggtg ccttggacac aatggctcat cgcggtgtac gggtggcgct cggctttgtt
      181 tgttctcgcc ggagtgctgg cgctcatcgt ggcgccagcg aatttcctat ttcaaagaca
      241 gaggccggag gagatgaacc tcaagccgga ttttggccag ccagggaaga tttccgttca
      301 cgaggtcaag gcaaaaactg cctccggtcc cacgttgcgg gacgcgctgc gcgattggcg
      361 cttctgggca tttgcggcag gcgtcttagc tggcgcgatt ccattacata tggttttgat
      421 tcatcaagtc gctgctgtca gcgatgccgg gttctcaaag gagtcggccg catgggcatt
      481 aggactgatc ggacttttta cagcgccggc gatgatcggg atgggattgt tggccgaccg
      541 catcgggcgg caatgggctt acgcgctcgg gtcaggggcg tttatgatcg gaattttttt
      601 cttgatgacg atcgacgcgg tgagcggcag ttggttgttc tatgcttttc caccgttcat
      661 cgcggttggc ttttcttcgc gccaatcgct ctacccgacg attgcggcgg atctcttcca
      721 cggcaaatcc ttcggcgcga tcatcggagc gctggctctg ttcatcggcg ccggcgccgg
      781 actcggcccg tggctcggcg ggcttatttt cgattggacc ggcggatatt actgggcatt
      841 ctgtatcgct caggggatga cggtggcgtc ggtggtcttt atctggcttg ccggctcgag
      901 ccggacgcac tgaacgattt gtgtcattgc gactgacgca ggataaaccc tatttcgact
      961 tgacgagcaa gaagcgggag ggtaataggt gccggattct ttgtatgcga ttaacaaatc
     1021 aatacccgga ggaccgattt aaatggaatg gaaagaagcg ctcccgttgt tgcgagaaaa
     1081 tcacaccgct gtcgccatta gcgtcacgcc gaaagggcat gctcagtcga ccatcgtgtc
     1141 gacagccttg ctcgatgaca agctcggctt cgcctcgcgg ccgcgaaccg tgaaacttaa
     1201 aaatatccaa cgaacaggcc gcgcggcgat taccgtgatc aagctcgata accggcgcta
     1261 tgtcaccgtg gaaggaccgg catccatcga gccatggcaa aacacgccgg cgcacatcaa
     1321 gcggctcaag gatttttatg cctcgatggg acgcgcgcag aagggcacag acgaggattt
     1381 tgcccgacag atgcgcgcgg aagagcgtag cctaattctg attacacctg agcacgtcta
     1441 cggcagcctg caatcgggcg catagctgat cgattcgccg ctcgatagcg cgaaaccgat
     1501 aaaccacaga gagaggaaga gtacagagag attttaagtt tttaatctcc tccgcgtttc
     1561 tctgcgcctc ggtggtgacc cgctagtttt tttcctagct cgtctaaaaa tgtgtggccg
     1621 ttcgggcggc gtaacgatcg attacgtgct gtgtcatgct ccgggcgagc tcttgctcgc
     1681 cgaagaagat cgcgccgatt ttttcctttt ccagcagagc ggattctttt tcactgtgcg
     1741 tacgcacaac gatttccacg cgcggattga ccgttcgcgc agtctcgatg atgcggcgca
     1801 cttgcacgat atccgggatc gccgtcacca gcatgcgggc acgatggatg tgcgcttgaa
     1861 tgagcacggc cggctccgag gcgtcgccgg aaacggcggg aatatctctt tcgcggagct
     1921 tttcgacgat ttcacgattc tcctcggcaa cgacaaacgg aatgccttgc gccgtcaatg
     1981 ccgcaccaat gcgacttcct actcggccat atccgaccag aacaacttgt ccggtgagcc
     2041 gcttgagatc tatggaagtc ggcaatgcgg cgagaggatc cgtcggacgc tccaaagcct
     2101 gggccagctt ggagcgggag cgaatccagt tttgcacggg ctcgatggca cgaaatacga
     2161 gcggatttag tccgatcgag atgatggctc ccgcgaggac gaggctctga ccctcggccg
     2221 gcatgagctt cagggaaacc gccaatccgg cgaggataaa tgaaaactcg ccaatctgag
     2281 cgagactcgc cgaaacgata agcgcggtgt tgagcggata gcgaaacagc agcactaaaa
     2341 agaaagcggc cagggactta ccgacgatga taatcgcgag gacgatcagg acgtggagcg
     2401 gttctcgaat cagcacccga ggctcgaaaa gcattcctac cgaaacaaaa aagaggactg
     2461 aaaaggcgtc tcggaatggc aaagattctt cggccgcccg gtagctcaac ggtgattcgc
     2521 gcattaccat gccggcgaaa aatgcgccga gagcaaagga tacgccgaac aacgcagctg
     2581 agccgtaggc gatgccgacc gcggctgcga tgatgcaaag agtaaaaagc tcccgcgagc
     2641 cgacgcgcgc gatttgccag agtagccatg gaaaaaaacg gcgtcccacg agcagcatga
     2701 gcccaataaa aactgccact cttgcgaggg ccagccccaa cgttgccgat aagctgcggt
     2761 cgatactttc ggcagtagtt ccaggaccat gtataccgtt ccacgccccg atagacggca
     2821 agagcaccag gaccagaacc attgcgaggt cttccacgat gagccagccg acagcgatcc
     2881 tgccgttgag tgttgccaaa atatcacgat cttcgagagc tcggagcagc acgacggtgc
     2941 tggcgaccga cagcgcgaga ccgaacgaaa tgctggcggc aatattccat ccccaggaat
     3001 gagcaaccgc agcgccgaga gcagtggcgg cagcgatctg cacgatcgcg ccgggaagcg
     3061 caatacgtcg aacggcgagg atctcatcga atgaaaagtg cagaccgacg ccgaacatca
     3121 atagcatcac gccgatttca gcgagctgct gtgagagccc cacatcggca acgaaacccg
     3181 gcgttgccgg accgatgata attccggcaa ttaaataacc gaccagaggc ggcagtttga
     3241 ggcgaattgc gacaaagccc atcgcgagcg ctaggccgag cgcagcggcg atggtagtga
     3301 tcaagctgac gttatgcggc acggcccatt ctcccattta agaagctgaa ttaaagtacc
     3361 gaaccaagaa gccgtattgc tcagacgatg gcgtacttaa ccagccccta ctccgattgc
     3421 aatcccgccc attgacttcc gggtacagtg cgcgtgcaca atgaggtatg cgtcttcccg
     3481 tgaacccgcc ggttttgccc atgctcgcca agcgagtcag cgagttaccg cgtgcgggag
     3541 cgtggatttt tgagcccaaa tgggatggtt ttcgcgcgct ggtatttcgg gatgcggacg
     3601 aaatccatat tcagagccgc gatgaaaaat cattcaatcg ctattttccg gaacttctcg
     3661 cgccgctttg ctcacaattg ccggcgcgct gtgtgctgga cggcgaaatt gtcatcgctc
     3721 agaatggcgc gctggacttt gatgcgctac agctgcgcat tcatccggcc caatcacggg
     3781 tcaagctgct tgcaaaagaa acgccggcat cgatcgtgtt cttcgatgct ctcagtgaag
     3841 gagaccggga tttacgcggc gccgcgtttg aagagcgccg atccattctg gaaaggctgc
     3901 tttcgtctgc caaagcgccc attcatctga cgcccgcgac ccgcgaaacg aaggtcgcgg
     3961 cggattggtt tcgtcggttc gaaggcgccg gtctggacgg cgttgtcgcc aagccggtgc
     4021 ggggaatcta tgaaccgaac aagcgtgcga tgctcaaaat caagcacgag cgcgagtgcg
     4081 attgtgtggt tgctggattc cgctggcaca agaagggtga gcgcacgatg ctgggctcat
     4141 tgcttctcgg gctctacgac gattccgggg agctgaacca tgtcggcgtc tgtgcgagct
     4201 tcacggaaaa aaaacgccgc gagctcgtcg agtttcttgc accgtaccgc aaaaatgcgc
     4261 tcgtccacca tccttggaaa aattgggcgg agcaagccaa cggggaagcc gattatcgca
     4321 tgccaggcgg ccagagccgc tggagctcgg gtaaagattt gtcctgggag ccgctgcgtc
     4381 cggaactggt tgtcgaagtt gcgtatgacc atatgcaagg cgatcgattc cgtcacacgg
     4441 cgcagtttcg ccggtggcgc atcgacaaaa agccgaaaga ctgcacctac gatcagctcg
     4501 aagtcgttgc gcctgaagaa ttggcgacga tcttcgccag cggacggtag tgtactgtga
     4561 ctaaagtcat cttccctgtc ctgcggtata actccggcca tagaaaagat cggttaaaaa
     4621 gcctatccct tgcagctaac ggccgattcg tagccgcgac gaaaacaaaa ccgagcgaaa
     4681 actttgaacc cggaccttga actcggaacg ttagtagaat gggaggacga gatctatgaa
     4741 aaaggtcacc ttcgtttcac tcatcttaat cctgtttacg gcaggatggt gcgcgggcgc
     4801 tgagcgcatg cgcatcggct acagctcgat cagcggatcg tacctgggaa tctgggtcgc
     4861 tcacgacgcc ggctatttcg ccaaagagaa gctcgaggat cagatcatct taatcccgag
     4921 cggcagccaa cttgcacagg tc
//
LOCUS       DASULZ010000180         3021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1341046, whole genome shotgun sequence.
ACCESSION   DASULZ010000180 DASULZ010000000
VERSION     DASULZ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341046"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VFY96_03680"
     CDS             complement(<1..429)
                     /locus_tag="VFY96_03680"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX5605588.1"
                     /translation="MLEAFIIGFWKVLAWPAFGYMLIGIAIGFWVGLLPGIGGLSTLA
                     LMLPFSYNIKDPVSAFAFLLGMLAVTGTTGDLTSILFGIPGEGSSAALILDGYPMSKN
                     GEAGRALGAAIMSSVVGALIGAFSLALSIPIMRPLVLLFGS"
     gene            complement(651..1745)
                     /locus_tag="VFY96_03685"
     CDS             complement(651..1745)
                     /locus_tag="VFY96_03685"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5605589.1"
                     /translation="MTPRNHSPLDGSHGHRVSFLLLSMLTGLTVVGTSGLKPALAQGD
                     FYRGKTIRMIVGYQAGDNHDLWMRFYARFLAKQIPGNPDFVVQNMPGAGGMIAANHVY
                     NLTKPDGLTLATIGGALFMAQLTGRKEVQFDWPKFSWIMTPERGGHLLFMRTDAPFKS
                     LDDLRQTNDPPRCSATGVGSTGYDIPRLLEEAFGFKFRVISGYPGGAEQDLAMERNEV
                     QCRAITIDGFFGREPFHTWTKKGFVRVVLQTEKKRHDKLPNVPTIFELMEQYKISDTK
                     RRLINSYLGIWGFGSRPIVSTPNVPPERLKILRDAFMRMFKDPEVLEEMKRRGWEAEP
                     VSGDELENLAKDVVDQPPEIAAALKRILAP"
     gene            complement(1767..2801)
                     /locus_tag="VFY96_03690"
     CDS             complement(1767..2801)
                     /locus_tag="VFY96_03690"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5605590.1"
                     /translation="MRQRLVFAAIAIVLAASPGYPQTPFYQGKTIRIVVGNLPGDTHD
                     LFARAYSRSMGKHIPGNPSMLVQNMPGAGTMIAANHVYNIAKPDGLTLGSVSPALYYA
                     QLTGSKEVKFDWPKFTWIGSPERDGSLLFMRADSPYKTLEDIRNAKEPPRCSATGVGT
                     NGHDIPKLFEETIGLRFQIITGYPGGAEQDLAMERNEVQCRAITIAAFFAREPFISWY
                     KKGFVRVLIQTPRQRNPKIPDVPTLYELMEQYKTPETKRRQAFVYLGAGGFGSWPILA
                     TPGIPADRATLLREAFNNTVKDPDFTAEANKNGWEIRPVRGEELQALAKDVVNQPPEV
                     VEWLKRLLVK"
BASE COUNT          654 a          831 c          893 g          643 t
ORIGIN      
        1 cgagccgaaa agcagcacga gcgggcgcat gataggaatc gaaagtgcca gtgaaaaagc
       61 gccgatcaac gcgcccacga cggagctcat gattgcggcg cctaaggcgc gccccgcttc
      121 gccgttttta gacatggggt agccgtcgag gatcaaggcg gctgaggacc cctccccggg
      181 gatcccgaaa agaatcgagg tgagatcgcc ggtggtgccg gtaacggcga gcataccgag
      241 cagaaatgca aaggctgaaa caggatcttt gatattgtaa gagaacggca gcatcagcgc
      301 caaagtcgac aagcccccga tgcccggaag aagaccaacc cagaaaccga tagcgatgcc
      361 gatcagcatg taaccaaagg ctggccatgc cagaactttc caaaatccaa tgataaatgc
      421 ctcaagcatg tcggtctcct agatcaaatg ataccgctgc ggagcctgct ccttaccgca
      481 taatcgaaga agcgtcaata tgattcccgc cggcttggcg tatcggcctc ggcgcaattc
      541 ttcagctgaa aatgcggctc gctgaagagc gtaaaatcaa aggaagaata acgcgaaggg
      601 tttcggatgt gcttgacggc gtctcgtttg tgccgcgaaa attttcgcgc ttacggggcg
      661 aggatccgtt tcaacgcagc ggcgatctca ggcggttggt cgacgacgtc tttggcgagg
      721 ttttcaagct catcgccgga aaccggctca gcttcccagc cgcgcctttt catttcctcg
      781 agcacctccg gatctttgaa catgcgcatg aaagcgtcgc gaagaatttt caaacgctcc
      841 ggtggcacat tcggcgtcga cacgatcggc cgcgagccaa agccccagat gccgaggtag
      901 gaattgatga ggcggcgttt ggtgtcgctg attttgtatt gctccatcag ctcgaaaatc
      961 gtcggcacgt tcggcaactt gtcgtgacgt ttcttttcgg tctggagaac gactcggaca
     1021 aagccctttt ttgtccaggt gtgaaacggt tcccggccga aaaaaccgtc gatcgtgatc
     1081 gcccggcact gaacttcgtt gcgctccatc gcgagatcct gctccgcgcc gccgggatag
     1141 ccgctgatca cgcgaaactt gaagccaaac gcctcttcga gcaagcgcgg aatgtcgtat
     1201 ccggtgctgc cgacgcccgt ggcggagcag cgcggcggat cattggtctg gcgcagatcg
     1261 tcgaggctct tgaacggcgc gtcggtgcgc atgaacagca gatggccgcc gcgttcgggc
     1321 gtcatgatcc aggaaaactt cggccaatcg aattgcactt cttttcggcc ggtgagctgc
     1381 gccataaaga gcgcgccgcc gatggtggcg agcgtgagcc cgtcgggctt ggtcaaattg
     1441 taaacgtgat tcgccgcgat cataccgccc gcgcccggca tattttgcac gacgaagtcg
     1501 ggattgccgg gaatctgctt ggcgagaaac cgagcataga atcgcatcca gagatcgtga
     1561 ttgtcgcctg cctgatagcc gacgatcatg cggatcgttt tacctcgata gaaatctcct
     1621 tgcgcgagtg caggtttcaa accggacgtg cccacaaccg tcaagccggt cagcatcgaa
     1681 agaaggagga aactcacccg gtggccgtgt gaaccgtcga gcggcgagtg gtttcttgga
     1741 gtcataggga gcctcattct tatcgtttat tttaccagga gcctttttag ccactcgacc
     1801 acttccggcg gctgattgac gacatccttt gccagggcct gaagctcttc gccgcgcacc
     1861 ggccttattt cccaaccgtt tttgttcgcc tcggccgtga agtcgggatc tttgacggtg
     1921 ttgttgaacg cctcacgcag cagggtggct cggtcggcgg gaatgccggg ggtcgcgaga
     1981 atcggccagg agccaaaacc accggcgccg agataaacga atgcctggcg ccgcttcgtt
     2041 tcaggagttt tgtattgctc catcaattcg tagagcgtcg gcacgtcggg aattttggga
     2101 ttgcgctggc gcggcgtctg aatcaacact cggacaaagc cttttttgta ccagctgatg
     2161 aaaggctcgc gcgcaaaaaa agccgcgatc gtgatcgcgc gacactgaac ttcgttgcgc
     2221 tccatggcga gatcctgctc ggcgccgccg ggatatccgg tgatgatctg aaagcgcagg
     2281 ccgatggttt cctcgaagag cttgggaatg tcatggccgt tggtgcccac gccggtggcg
     2341 gagcaacgcg gcggctcttt ggcgttgcgg atatcttcga gcgttttgta gggtgaatcg
     2401 gcacgcatga acaacaacga tccgtcgcgc tccggcgaac cgatccaggt aaacttgggc
     2461 cagtcgaact tgacttcttt gctgccggta agctgggcgt agtagagcgc cggcgagacc
     2521 gagccaagtg tcaagccatc gggcttggca atgttgtaca catgattggc ggcgatcatt
     2581 gtgcctgcgc ccggcatgtt ctgaaccagc atcgaaggat tccccggaat gtgtttgccc
     2641 atgctgcgcg agtaggcgcg cgcgaagaga tcatgagtat cgcccggtag gttgccgact
     2701 acgattcgaa tggttttccc ctggtaaaag ggagtctgag gatagccggg actagcggca
     2761 aggacgatcg caatcgcggc gaaaactaat ctttgtcgca tcgcgagctc attacagtta
     2821 aaaaaccgca ttcgtggagt tcggagtaat ggaatgctgg gcgccgcttg ccggtgttgc
     2881 atcgctcatt gggtcaaccg gtcattttcc taagacccac ttcatccgct cgacgacttc
     2941 gcgcggttgg ttgatcactt ctttagacaa agtttcaagc tccgcgccac ttaatggatc
     3001 caaatcccag ccgcgcttgg c
//
LOCUS       DASULZ010000181         4752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_22112, whole genome shotgun sequence.
ACCESSION   DASULZ010000181 DASULZ010000000
VERSION     DASULZ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22112"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VFY96_03695"
     CDS             <1..663
                     /locus_tag="VFY96_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX5605591.1"
                     /translation="LVGAANLQNILGAIGVASALKLDPRAIADGVDKLRSVPGRLEKV
                     PNRLGITILVDYAHSPDALEKVLGAVRPLTRGKLITVFGCGGDRDRGKRPVMGEIAGR
                     LSDVTVLTSDNPRSESPMAILDEVEAGMRRTEVIKLSVSSLQSRVVETRKPIPETERG
                     YFVEADRRTAIEIALSIARPGDLVLIAGKGHEDYQILGSERIHFDDREVAREQAEERA
                     GV"
     gene            676..2079
                     /gene="murF"
                     /locus_tag="VFY96_03700"
     CDS             676..2079
                     /gene="murF"
                     /locus_tag="VFY96_03700"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX5605592.1"
                     /translation="MGWGKQELISATGGKILRDGKQMAFGEIVTDSSKAQAGSVFIAL
                     KGERHDGHRFIGDAVRRGATFVILHRLPKKIPAGEAVIIKVPDTLRALGDLAHYRREQ
                     IAPKVLAITGSNGKTTTKEMLAAILEEASLNGKRLRGRVLKTEGNFNNLVGLPLTLLR
                     LREDDQIAVVELGTNHPGEIQRLAEIADPDGGIITSIAGAHLEGLRSLAGVAREKGAL
                     YRNVRADGAIALNLDDPWVRRLGANFKGRKITYGKRGWVRALSPRLRATQGMQFKLQA
                     GSHTAHVRLHYLGQHNINNALGAAALALGAGVDLAAVRRGLGKARPFSMRMEVDQWNG
                     VGIINDAYNANSASMRAALQTLAELPCRGRRVAVLGDMFELGKHNAKEHRQVGKAAAR
                     AAIDGLYLLGKQATTMRRAAVAHGMRPEQVTIGQDHTDIARQLRQQLKKGDWLLVKGS
                     RGMKMETVLHELKSGKA"
     gene            2090..3166
                     /gene="mraY"
                     /locus_tag="VFY96_03705"
     CDS             2090..3166
                     /gene="mraY"
                     /locus_tag="VFY96_03705"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX5605593.1"
                     /translation="MLYYLSFPLSMTYSGFNVFRYITFRAAMAALLALLISFLLGPWL
                     IRTLTQKQIGQQIRDDGPASHAVKAGTPTMGGALIILALFFSTLMMADIANRYVLLAL
                     LATLGTGVVGFTDDYLKLTRKNSRGLSARGKLLGQLLVAFIVAVALYYQPRFSTDIAV
                     PFVKTFHFELGPFYVPFAMLVIVCTSNAVNLTDGLDGLAIGPVMIAAGTYAVIAYVTG
                     HLKLAEYLQIPYVAGVGELSVFCAAVVAAGLGFLWYNAYPAQMFMGDVGSLSLGAALG
                     VVAVMTKNEILLIIVGGVFVMEALSVIFQVISYKLRRKRVFLMAPIHHHYELKGWKEP
                     QIIVRFWILSFICAVVGLSTLKLR"
     gene            3173..4507
                     /gene="murD"
                     /locus_tag="VFY96_03710"
     CDS             3173..4507
                     /gene="murD"
                     /locus_tag="VFY96_03710"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX5605594.1"
                     /translation="MELKDKTVLVVGLGRTGRDSARFLAQQGARVLVNDFRPAAELRS
                     ETNDLSDLAIEYRLGGEHSDYLNGIDYVIPSPGVARENVLLQEASARRIPILSEIELA
                     YRFIRAPLIAITGTNGKSTATTLVGEMLRAAGKKVFVGGNLGVPFISAVSDDWDWTVL
                     EISSFQLEWVKQFRPRVAVLLNLSEDHLDRYASFAEYGATKARIFEAQTSSDTAILNR
                     DDPSVWEMRKRLKSRVVSFGFAEAGAGVFAAGNELIWRDGGAEERFSLADVKLHGIHN
                     LENMMAAVAAAKSIGFARDAIQTVLNRFPGLEHRLEFVREKDGVAYYNDSKGTNVGAV
                     VKSLESFSAPVVLLAGGVDKGGSYAPLERQIKEKVRRLVLFGAAKEIIARALGALTDT
                     VIVEDLPTAVRDAAAHSQPGDVVLLSPACSSFDQFKNYVERGRAFKTLVQEL"
     gene            4504..>4752
                     /locus_tag="VFY96_03715"
     CDS             4504..>4752
                     /locus_tag="VFY96_03715"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX5605595.1"
                     /translation="MRDGLTVDKWMLLAVVGLLAVGVTMVLSTSYLYSQERYGDGTYF
                     LRKQMIAMGAGAVTLIACTMVPSALYRRFAYPILALSFI"
BASE COUNT          985 a         1256 c         1452 g         1059 t
ORIGIN      
        1 cttgtgggcg cggcaaattt acaaaacatc ttgggcgcga ttggcgttgc ttcggcactg
       61 aaactggatc cgcgagcaat cgccgacggc gtcgacaagc tgcgctcggt tccgggtcgt
      121 ctggaaaaag ttccaaatcg cctcgggatt acgattttag tcgactatgc ccattcgccg
      181 gacgcgctgg agaaagtcct gggcgccgtc cggccgctca cccgcggaaa actgattacg
      241 gtctttggct gcggcggcga tcgtgatcgc ggcaagcggc cggtcatggg cgaaattgcc
      301 ggccggttga gcgatgtcac ggtgctgact tccgataatc cgcgcagtga aagcccaatg
      361 gcgattctcg acgaagttga agccgggatg cgtcgaacgg aagtcatcaa acttagcgtc
      421 tcgagtttgc agtctcgggt cgtcgaaacc cgaaagccga tacccgaaac cgagcgcggt
      481 tactttgtgg aagccgatcg acgtacagcg atcgagatcg cgctgagtat cgcgcggccc
      541 ggggatctgg tcttaatcgc cggcaaaggg cacgaagatt atcaaatttt gggcagtgag
      601 aggatccatt tcgacgatcg ggaggtcgcc cgcgaacaag cggaggagcg cgccggcgta
      661 taagcagcac gaggaatggg ctggggcaag caagaactga tcagcgccac gggcggcaag
      721 atcctgcgcg atggaaagca aatggccttc ggtgagattg tgacggactc gagcaaagct
      781 caagccgggt ccgtttttat cgccttgaag ggcgagcgcc atgatggaca ccgctttatc
      841 ggagacgccg tgcgccgcgg cgccacgttc gtcatccttc atcgcctgcc aaagaaaatc
      901 ccggccggcg aggcggtaat catcaaagtg cccgacacgc ttcgcgcgct gggagatctt
      961 gcgcattatc ggcgcgaaca gatagcgccc aaagttttag cgattacagg ttcaaacgga
     1021 aagaccacga ctaaagagat gcttgcggcc attctcgaag aagcatccct gaacggcaag
     1081 cgattgcgcg gtcgcgtgtt aaagaccgag ggcaacttta acaatctcgt gggtctgcca
     1141 ttgaccctct tgcgcttgcg tgaagacgac cagatcgccg tcgtggagct cggcaccaat
     1201 catccgggtg aaattcagcg gcttgccgaa attgccgatc ctgacggcgg aatcatcact
     1261 tcgattgcag gtgctcacct cgaagggctg cgcagcctgg ccggcgtagc gcgcgaaaaa
     1321 ggcgcgcttt accgaaatgt tcgcgccgat ggcgccatcg cgctcaatct cgatgatcct
     1381 tgggttagaa ggctcggcgc gaatttcaaa ggccgcaaga ttacgtacgg caagcggggc
     1441 tgggtgcgag cgctgtcgcc gcggctgcgc gctacgcaag gcatgcaatt caagcttcaa
     1501 gccggcagcc atacggctca tgtacggctg cattatctcg gccagcacaa tatcaataat
     1561 gccctgggtg ccgcggcttt agccttgggt gcgggcgtgg atctcgcagc ggttcggcgc
     1621 ggacttggca aggctcggcc tttttcgatg cgtatggaag tcgaccagtg gaacggcgtc
     1681 gggattatca atgacgccta caacgcaaat tcggcatcaa tgcgcgcggc gctgcaaacg
     1741 cttgcggaat tgccctgccg cggtcggcgc gtcgccgttc tgggagacat gtttgaactg
     1801 gggaagcaca acgcgaagga gcatcggcag gtcggcaaag cggcggcgcg tgcggcaatt
     1861 gacggtttgt atctcctcgg gaagcaagcg acaacgatgc gtcgcgccgc cgttgcccat
     1921 ggcatgaggc cggagcaagt taccattggc caggatcata ccgacatcgc tcgtcagctg
     1981 cggcaacaat tgaaaaaggg cgactggctg ctcgttaaag gatcccgcgg gatgaaaatg
     2041 gaaaccgttt tgcacgagct taaaagcgga aaggcatagg agattggagt tgctctacta
     2101 cctttcgttt ccgctttcca tgacttactc gggcttcaat gtttttcgtt acatcacgtt
     2161 tcgtgcagcg atggcggcgc tgctcgcatt gctgatctcg tttttgctcg ggccctggtt
     2221 gatccgtact ttgactcaaa aacaaatcgg ccagcaaatc cgggatgatg gtcccgctag
     2281 tcatgcggtg aaagcaggaa cgccgactat gggcggcgcg ctgatcatcc tggcgctttt
     2341 cttctcaacc ttgatgatgg ccgacatcgc caatcgatat gtcttgcttg cgcttctcgc
     2401 caccttgggt accggcgtgg tcggtttcac cgacgactat ctgaagctga cccggaaaaa
     2461 tagccggggc ctttcagctc ggggaaaact gctcggccaa ttactggtcg ctttcatcgt
     2521 cgccgtggcg ctctactatc aaccccgatt cagcacggat atcgccgtgc cgttcgtaaa
     2581 aacttttcat ttcgaactcg ggccgtttta cgttcctttt gcgatgctcg tcattgtttg
     2641 tacctcaaat gcagtcaatc tgaccgacgg cttggatgga ttggccatcg gtccggtcat
     2701 gatcgccgcg ggaacctacg ccgtgatcgc gtacgttacc ggacatctga agttggccga
     2761 gtatctgcaa attccgtacg tggccggagt gggtgagttg agcgtctttt gcgcagcggt
     2821 tgttgccgcg ggactgggct tcctttggta caacgcttat ccggcacaga tgttcatggg
     2881 tgatgtcggt tcgctttcgc tcggcgccgc cctgggtgtc gtcgcggtga tgacgaagaa
     2941 cgaaattctt ttaatcatcg tgggcggggt cttcgtcatg gaggcgttgt cggtcatctt
     3001 tcaggtgatc tcgtacaagc tcaggcgaaa gcgggtattt ttgatggcgc cgattcatca
     3061 ccattacgag ctcaagggct ggaaagagcc gcagatcatc gtgcgcttct ggattctgtc
     3121 atttatatgc gccgtggtcg gtttgagcac attgaagctt cggtgatgaa gcgtggaact
     3181 gaaagacaag acagtgctcg tggtcggatt aggccggacc ggtcgggaca gcgcgcgttt
     3241 cctggcgcag caaggagcgc gtgtgctggt caatgatttt cgtcccgcgg cggaactccg
     3301 atcggaaaca aatgacctct cggatctcgc gatcgagtat cgattgggcg gcgaacactc
     3361 ggattacctc aacggcattg attacgtgat cccgagccca ggtgtggcgc gcgagaacgt
     3421 tttgctgcaa gaagcatcag ctcggcgaat cccgattctg agcgagatcg aattagctta
     3481 ccggtttatt cgtgcgccgc ttatcgcgat caccggtacg aacggcaaaa gcaccgcgac
     3541 cacgctggtc ggcgaaatgc tcagagccgc cggcaaaaag gtttttgtcg gcggaaatct
     3601 cggcgtgccg tttatcagcg ccgtgtcaga cgactgggat tggaccgttt tggagatcag
     3661 cagttttcaa ctggagtggg tgaagcagtt tcgtccgcgc gtcgcggtgt tgctcaatct
     3721 cagcgaggat catttggatc gctatgcgtc ctttgccgag tatggcgcga ctaaggcacg
     3781 aatctttgaa gcgcaaacga gctcggatac ggccatcctc aatcgcgacg atccgagcgt
     3841 ctgggaaatg cgcaagcggc tcaagtcgcg cgtggtttcc ttcggctttg ccgaagccgg
     3901 cgcgggtgtg tttgcggcgg gaaatgagct catttggcgc gacggcggcg ccgaagagcg
     3961 attttcgctc gccgacgtca agcttcacgg catacacaat ctggaaaata tgatggcggc
     4021 agtggcggcg gccaaaagca tcggtttcgc gcgcgatgcg attcaaacgg ttttgaatcg
     4081 ttttcccggt ctcgaacacc gtctcgaatt tgtccgcgag aaggatggcg tcgcttatta
     4141 caacgattca aaaggaacca atgtcggtgc cgtggtgaaa tcactggaga gcttttccgc
     4201 gccggtcgtt ttgctcgcgg gcggggtcga taagggcggc agctacgctc ccctggagag
     4261 acaaatcaag gaaaaagtcc gccgcctggt cctgttcggc gccgccaaag agatcatcgc
     4321 gcgagcgctg ggcgcgctca ctgatacggt gatcgtcgaa gatttaccga cagcggtgag
     4381 ggatgctgcg gcacattcgc aaccgggaga tgtggtgtta ttgtcgcccg cctgctcgag
     4441 cttcgatcag ttcaaaaact acgtcgaacg aggaagagcc ttcaagactc tggtgcagga
     4501 actatgagag acggtctgac agtggacaag tggatgctcc ttgcggttgt gggtcttttg
     4561 gcggtcggtg tgacaatggt tttgagtacc agctatctct attcgcaaga acgctatggc
     4621 gacggcactt attttcttcg caaacaaatg atcgcgatgg gtgccggcgc tgtcacgctg
     4681 atcgcctgca cgatggttcc ttccgcgctc taccgaaggt ttgcttatcc gattctggcg
     4741 cttagtttta tt
//
LOCUS       DASULZ010000182         5946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1239223, whole genome shotgun sequence.
ACCESSION   DASULZ010000182 DASULZ010000000
VERSION     DASULZ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5946
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239223"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VFY96_03720"
     CDS             <1..352
                     /locus_tag="VFY96_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605596.1"
                     /translation="VAGQAAACLSCYAHHYPIRKRKGAPVDYMLTEGTGDIDATAILK
                     TAPHPNTALLFARWTASEEGQKTYAQGGRLPAHPKIEPVEKTRPAKVYPVGENDVKDY
                     PKYEKIWKEIFGLR"
     gene            complement(399..2087)
                     /locus_tag="VFY96_03725"
     CDS             complement(399..2087)
                     /locus_tag="VFY96_03725"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5605597.1"
                     /translation="MYDLILKGGRIYDGTGMPSFLGDIAINDGRIEAIGRINAYADHV
                     LDVDGLAVAPGIIDFHTHFDAQLWWDPLASSSNEHGVTTVVMGNCGLTLAPCKPESRD
                     ALIGTFVRVEDMPRQSLQAGIPWAWTTHGEFLDALEAQPLGLNVATLIGHCAVRQYAM
                     GEASVEREANDAEIAEMEELVHHGMVAGAFGFSTNANQRHFREDGKPVPSRFAGLDEV
                     ARLCRTVGESRRGLVQFTHGAFASPEHVAHIGNWYDTILKETRRPLIGESIRHFWSEP
                     DLWRKQLRDVEARCRQGYPAYAMTSTRRSMRRWSLKDTTRLDEMPAWKSLLSSPLDLR
                     KEKLRDPQTRLELGRAIEFGKPIGFCRRWDLISVKKVARPENKSAENKSIDELAREKT
                     KPAIDAFLDLALAEDLETTFEDCASQGDIHAVKEIFSNPHVLLGQSDAGAHVANVSPG
                     FGFSTMMLADWVRERRIMSLEEAIKKMTFLPASIFGIHDRGLVRRGMAADLFVFDPEK
                     IDLAEPEKVADLPEGASRYIQRAKGIHYTIVNGSVLMKNGAHTGVYPGKVLRSS"
     gene            complement(2402..3565)
                     /locus_tag="VFY96_03730"
     CDS             complement(2402..3565)
                     /locus_tag="VFY96_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEX5605598.1"
                     /translation="MKQSTHRILTTHCGSLARPTELLDVMKAKVNGEAYDAEKYAATL
                     RRSVADIVSKQAECGIDIVADGEQSKPGFFTYVRERLTGFEKKDAPKAAWTQWSGEVA
                     MFPEYYEQYFSRRMMGGSITARAPLVCTGPIAYRGQEAIRKDIENLKAALVGLNVEEV
                     FMPAVAPTGVGRNEFYRSEEEYLSAVGDALREEYKAIVDAGLILQVDDPWLTEVHSQD
                     PNLRPTERRKIAELSVEALNQAIRGIPAEKIRFHTCYGINEGPRIHDAPLKEIIDLIL
                     KVNAGAYAFEAANARHEHEYRVWEDVKLPEGKILIPGVITHCSNIVEHPELIAERIIR
                     FAKLVGRERVIAGGDCGFSSQATYNPEVHPKIVWAKFQAMAEGAKLASRQLWR"
     gene            complement(3655..4941)
                     /locus_tag="VFY96_03735"
     CDS             complement(3655..4941)
                     /locus_tag="VFY96_03735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(3892..3894),aa:Sec)
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HEX5605599.1"
                     /translation="MEKIRVVQYLNQFFAQIGGEDKADVGPQIRDGIIGPGRALQHAL
                     GEKGEVVATAFCGDNYFAERQEEVISDLIQRMAAYKPDLFIAGPAFESGRYGSACGAI
                     CDAVQRRLKIAVIAGMDEDNVGADLYRKRIYIVNSGPTIARMTPTLQRMAALGIKLAR
                     RESIGKPHDEGYLARGIKRNEFAAKPPAERAVDMLLAKLRGEAFHSEIAPPKFAGSKP
                     AAPLRDLKSAVIALVTDGGLVPEGNPDRIEPGRPTRFTTIPISGVTSLDADKYDAVHS
                     GYDTAIANRDPNRLVPLDAMRALEREGAIGKLHEFVHSTGGAHAAVENASRIGKEIAN
                     QLKSSGVTGVLLTSTUGSSTRCGAAISKEIEKLNIPVTQICTMIPVAKEVGSLRAIAS
                     GGIVSPTGNPALPPEKEKEFRRELILRALKTLQQDV"
     gene            complement(5019..>5946)
                     /locus_tag="VFY96_03740"
     CDS             complement(5019..>5946)
                     /locus_tag="VFY96_03740"
                     /inference="COORDINATES: protein motif:HMM:NF020896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HEX5605600.1"
                     /translation="NGPAAAYSPLCNTHNIVLVAHPKAGADRDDYRLAEKKAGLKAAT
                     YLAAAAQNVTPSEEQIFELPPVAANQGPKELPRIAYIFPMHSHQHPTQQNEPVFYGSN
                     IQGFMPTIVHPNEILDGALMFSYSAFTWFAQNHPVIHELYRRHGRDLWFAGVVLTIAP
                     VTIAEKERNTALAARLAKEVLNADGVIATKIGGGAVDTDLMMIYTRAEEMGMKAALII
                     MERYPDTGITFVPGNVNALVTPGLTRDPITLPAVDRVIGGDTIALDNADPDNSNPGVT
                     LVPANHELNLWIGDIVGAISQVGASRLTTYMS"
BASE COUNT         1165 a         1744 c         1687 g         1350 t
ORIGIN      
        1 ggttgcgggg caggcggctg cgtgccttag ctgttacgcg caccattatc cgatccgtaa
       61 acgcaagggc gctccggtgg attacatgct gaccgaggga accggcgata tcgacgccac
      121 ggcaatcctc aaaacggcgc cgcacccgaa caccgcgctg ctatttgcac gctggactgc
      181 cagcgaagaa ggtcaaaaga cctatgccca gggcggtcgc ctccccgcgc atcctaaaat
      241 cgaaccggtc gagaagaccc gccccgcgaa ggtctatccg gtcggtgaaa acgacgtcaa
      301 ggactatccc aagtacgaaa aaatatggaa ggagatcttc gggctgcgct gaatcgcacg
      361 cggtcgccgt cccgctcgaa ttcagcgggc gagtgccatc aagaactgcg caagactttt
      421 ccgggataga cgccggtatg cgcgccgttt ttcatcagca ctgatccgtt gacgatcgta
      481 taatgaattc ctttggcgcg ctgaatatag cgcgaggcgc cttcaggcag gtcggcaact
      541 ttttcgggct ccgccaaatc aatcttctct gggtcgaaca cgaagagatc cgcggccata
      601 ccgcggcgaa cgagaccccg gtcatgaata ccgaagatcg aggccggtaa gaaggtcatt
      661 ttcttgatcg cttcttcgag gctcatgatt cgacgctcgc ggacccaatc ggcgagcatc
      721 atcgttgaaa agccgaagcc gggactgaca ttggcgacat gggcgccggc gtccgattgg
      781 ccgagcagta cgtgcggatt gctgaaaatt tcttttaccg cgtgaatatc gccttggctc
      841 gcgcagtcct cgaatgtagt ttcgagatct tcggcgagag ccaagtccag aaaggcatcg
      901 atagccggtt tagtcttctc gcgcgcgagc tcgtcaatgc ttttattctc cgccgatttg
      961 ttctccgggc gcgcgacttt tttaaccgag atcaaatccc aacggcgaca gaaaccgatc
     1021 ggcttgccga attcaattgc tctgccgagc tccaatcgag tttgcggatc gcgcaacttc
     1081 tctttgcgca aatccagagg cgagctcagc aaacttttcc aggcgggcat ttcgtcgagg
     1141 cgagtggtgt ctttcaagct ccaacgccgc atcgaccggc gcgtactcgt catcgcatac
     1201 gcgggatagc cttggcggca acgcgcttcg acgtctctca gttgtttgcg ccacaagtcc
     1261 ggctcgctcc agaaatgccg gatgctttcg ccgattaacg gccggcgtgt ttctttgaga
     1321 atggtatcgt accagttgcc gatgtgcgcg acgtgctcag gggaagcgaa cgcgccgtgg
     1381 gtaaattgga ccaagccccg ccggctctcg cctaccgtcc gacacaggcg ggcgacttcg
     1441 tcgaggcctg cgaaacggct cgggactggc ttgccgtcct cgcgaaaatg acgctggttg
     1501 gcattggtcg agaagccgaa agcgccggcg accatcccgt ggtgcaccaa ctcttccatt
     1561 tcggcgatct cagcatcatt cgcttcgcgc tccacactgg cttcgcccat cgcgtattgg
     1621 cgtaccgcgc aatggccaat cagcgtggca acattcaatc ccaggggttg cgcctcgagc
     1681 gcatcgagaa attcgccgtg agttgtccac gcccacggaa tgcccgcctg caggctttga
     1741 cgcggcatat cctcgacacg tacgaaggta ccgatcaaag cgtcgcgact ttcgggcttg
     1801 catggcgcga gcgtcagacc gcaattgccc atgaccacag tagtgacgcc gtgctcgttc
     1861 gacgatgagg ccagaggatc ccaccagagt tgcgcgtcga aatgcgtgtg aaagtcgata
     1921 atcccgggcg ccacagcgag accgtcaaca tcgagcacgt gatcggcata agcattgatc
     1981 cggccgatgg cctcaatgcg accatcattg atcgcgatat cgccgagaaa cgacggcatg
     2041 cccgtgccgt cgtaaatgcg gccacctttg aggatcagat cgtacatagc atcacctcga
     2101 ccgatgattg agtcaccacg aagtgtttca taagtttata ctcgccacag agaatcaaaa
     2161 gggatacctc ccacgtattc ctacttcaag ttcgtcattc ccgcgtattt tttagcggtg
     2221 gttcgacggt gctcaacacc ggtgcattac gactggacct tgagtaaagc gttcggaggt
     2281 gatcggttga tgtacttcta ttcagtcgcg ccgttatttt cagacgagat acgaagcaag
     2341 ggtcggccaa aatcatgctc ctaagattcg cctgtcttcg ccccgcgtaa gggcagtgcg
     2401 cttaccgcca caattgtcgg ctcgccagct tcgctccttc cgccatcgcc tgaaattttg
     2461 cccagacgat tttcggatgg acctcggggt tgtaagtcgc ctgcgacgaa aaaccgcagt
     2521 cgccgcccgc gatcacgcgc tcgcgtccga ccagtttagc aaaacggatg attcgttcgg
     2581 cgatcagctc tggatgctcg acgatgttgc tgcagtgagt aattaccccg ggaatcaata
     2641 tttttccttc gggcagcttg acgtcttccc agacccggta ctcgtgctca tggcgggcat
     2701 tagccgcctc gaaggcataa gcgccggcgt tcacttttaa gatgagatcg atgatttctt
     2761 tcagcggcgc atcatggatg cgcggtcctt cgttgatgcc gtagcaggta tgaaagcgga
     2821 ttttctccgc tggtatgccg cgaatcgctt gattcagagc ctcgaccgac agctcagcga
     2881 tctttctccg ctcagtcgga cgtaggtttg gatcctggct gtgcacttcg gtgagccagg
     2941 gatcgtcgac ctgaagaatc aatccggcat cgactatcgc tttgtattcc tcgcgcagcg
     3001 catcgccaac ggcgcttaaa tattcttcct ctgagcggta aaactcgttt ctaccgactc
     3061 ctgtcggtgc gacggcgggc atgaagacct cctccacgtt caacccgacg agcgcggctt
     3121 tcaggttttc gatgtctttg cggatcgcct cctgtccccg atacgcgatc gggccggtgc
     3181 aaaccagcgg cgcgcgcgcg gtgatgctgc cgcccatcat gcgccggctg aaatactgtt
     3241 catagtattc cggaaacatc gcgacttcgc ccgaccattg cgtccacgcc gcctttggcg
     3301 cgtctttctt ttcgaaaccg gtgagccgct cgcgcacgta ggtgaaaaac cccggtttgc
     3361 tctgctcacc atccgcgacg atgtcgattc cgcattcggc ttgcttactg acgatgtcgg
     3421 ccacactgcg acgcaaggtc gccgcgtact tctccgcgtc gtacgcttcg ccattgacct
     3481 ttgctttcat gacgtcgagc agctcggtag gccgcgctag actgccgcag tgcgtggtca
     3541 ggatgcggtg cgtgctctgc ttcatgtcag ttctccttga ttccggattt agtttatcct
     3601 gagcctggcc gaaaggaccg gcatgactga cggctgcctc gaattaaccg aaaactagac
     3661 gtcttgctgc agggtcttca gcgcccgcag tatcagctcg cgcctgaact ccttctcttt
     3721 ttccggcggc aacgccggat ttccggtcgg gctgacgatg ccgccggaag cgatggcgcg
     3781 aagcgagccg acttctttcg caaccggaat catcgtgcag atttgcgtca cgggaatatt
     3841 gagtttttcg atttccttgc tgatcgctgc gccgcagcga gtgctggacc ctcaggtgga
     3901 tgtcagaagg acgccggtta cccctgagct tttcagctga ttagcgattt ctttgccgat
     3961 tcggctcgcg ttttccaccg cggcgtgcgc gccgccggtg gagtggacga actcgtgcag
     4021 ttttccgatc gcgccttcgc gttcgagtgc tcgcatcgcg tccagcggga ccaagcgatt
     4081 cggatctctg ttggcgatgg cagtatcgta accggaatgg accgcgtcat atttgtcggc
     4141 gtcgagagaa gtcacgccgg agatcggaat cgtggtaaag cgggtcggcc ttccgggttc
     4201 gatcctgtcc ggattgccct cgggtaccag gccgccgtcg gtgaccagtg cgatgaccgc
     4261 agatttgaga tcgcgtagcg gtgcagccgg tttgctgccg gcgaactttg gcggcgcgat
     4321 ttctgaatga aatgcttcgc cgcgcaactt ggcgagcaac atgtcgacgg ctcgctcggc
     4381 cggcggcttc gcggcaaact cgttgcgctt tatgccacgc gctagatatc cttcgtcgtg
     4441 cggtttgccg atcgattcgc gccgcgccaa tttgataccg agcgccgcca tgcgctgcaa
     4501 ggtcggtgtc atgcgcgcaa tggtcgggcc ggagtttacg atgtagatcc gttttcgata
     4561 tagatcggcg ccgacattgt cttcgtccat acccgcaatt acggcgatct tgagccgccg
     4621 ctgcactgca tcgcagatcg cgccgcaagc gctcccataa cggccgctct cgaacgctgg
     4681 accggcgatg aagagatccg gtttatatgc ggccatgcgc tgaattaggt cactgatcac
     4741 ttcctcttgt cgctccgcga aatagttatc gccgcaaaat gccgtcgcaa cgacttcgcc
     4801 tttttcgccg agcgcatgct gcagcgcgcg tccgggaccg ataatcccgt ctctaatctg
     4861 cggccctacg tccgctttgt cttcgccgcc gatctgggca aaaaattgat tgagatactg
     4921 aaccacgcga atcttttcca tctcgtttcc tcgattcgtc aggagtcagg cgtaaggggt
     4981 gaggcggggg acgccttacc acttacgatt tacgctcgtt aactcatata cgtcgtgagc
     5041 cgtgacgcgc cgacctgact gatggcgcca acgatatcgc cgatccacag attcagttcg
     5101 tgatttgctg gaacaagagt cacgccggga ttgctgttgt ccggatcggc gttgtccagc
     5161 gcaatggtgt cgccgccgat gacacgatcg accgccggca gcgtgatcgg gtcgcgggtc
     5221 aggccgggcg tcacgagcgc attcacattc cccggcacga aggtgatgcc agtatctgga
     5281 tagcgttcca taatgatcaa cgcggctttc atgcccattt cttcggcgcg cgtatagatc
     5341 atcatcaaat cggtatcgac tgcgccgcca ccgatcttcg tggcaatcac gccatcggcg
     5401 ttcagcactt cctttgctag acgagcagcg agagccgtgt tgcgttcttt ttctgcaatg
     5461 gtcaccggag cgatcgtcag caccacgcca gcgaaccaaa gatcgcggcc gtgacggcgg
     5521 tagagctcgt gaatgaccgg atggttctgc gcaaaccagg tgaacgccga atacgaaaac
     5581 atcagcgcgc catcgagaat ttcgttggga tgcacgatgg tcggcatgaa accctgaatg
     5641 ttgctgccgt aaaaaaccgg ctcgttctgc tgggtcggat gctggtgcga gtgcatcggg
     5701 aaaatgtagg cgatgcgcgg caattccttc ggaccttgat tggctgctac gggcggtagc
     5761 tcgaagatct gttcttcgga cggcgtaaca ttttgggccg ccgccgcaag atatgtcgcc
     5821 gcttttaagc cggctttttt ttctgccaaa cgatagtcgt ctcggtcggc tccagccttc
     5881 ggatgcgcta ctaagacgat attatgcgtg ttgcagagag gcgagtatgc ggcggccggg
     5941 ccgttc
//
LOCUS       DASULZ010000183         2607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_731983, whole genome shotgun sequence.
ACCESSION   DASULZ010000183 DASULZ010000000
VERSION     DASULZ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731983"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..669
                     /locus_tag="VFY96_03745"
     CDS             208..669
                     /locus_tag="VFY96_03745"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX5605601.1"
                     /translation="MISLEQANRIIAAIFARAREQSCRPMSVIVVEPGAKIKAFQKED
                     DSAMMRFEVAFGKAYAALALGRSSSLVRMRTEERPLFMQYLIRASDDQIFPEGGGMLI
                     RDPDGELLGAVGVTGDTQERDEELAAHGIRAAGLKTDEDCAQFGSKVRLKN"
     gene            complement(743..2446)
                     /locus_tag="VFY96_03750"
     CDS             complement(743..2446)
                     /locus_tag="VFY96_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5605602.1"
                     /translation="MNKPAQPPSSTITGAEAVVEMLQAHEVELIFGLCGDTSLPFYDA
                     LARLPHGMRHILARDERSAAYMADGYARVSKKVGVCEGPSGGGVTYIVPGLAEANESS
                     VPLLCINSDISVAARGRYTLTALDQGALMRLITKWNTVLDRAEDIPAVFRQAFRQSTT
                     GRPGAAHIALPFDVQNGLVNRTEVWGETKFGSCPAERSAPEVDSIERAARLLRAAARP
                     VFICGGGVVVSGAEPELLALAERISAPVATTISGKGSIDEQHPLAAGVVGSNGGTPET
                     RSIVDQADLVIFVGCRAGSVTTERWRHPVPGQVKIVHLDVDSAVPGTNYPTDAAVVGD
                     AKLALRMLDEALGDLRRPLDAALVQNAKEHKFAKFQALAESAEKPIKPERVVAALAGA
                     LDPDAIVVADAGTPCPYLSAYYELGGSGRRFFSNRAHGALGYSLSAAIGAHLAKTAVK
                     CVSVMGDGSFGFSCGELETAARYCLPITFVVIANASYGWIKAGQKSGYGKRYFSVDFA
                     ATDHAAVASAFGVKSWRVTDPVNVAMTLKEALAYGGPSLVDIVCQPLEEAKAPVSEWI
                     A"
BASE COUNT          537 a          776 c          790 g          504 t
ORIGIN      
        1 cgcgcgtaaa gcgatggtgc aaatattcga cgttttaccg ccgggtgacc cgctggtcga
       61 taagtaccga tctgagcttg ctaaggttct gttccgctaa agttcctagc gaacggctac
      121 aaaccttcgg cttggcaggg ccaaagttag atgtggtcaa tcatcgcaga tgactgaatc
      181 gaagcttgcg caagcgagga ggctgggatg atttctctgg agcaagccaa tcggatcatc
      241 gcggccatct ttgcccgcgc tcgtgagcag agctgccggc ctatgagcgt gatcgtcgtc
      301 gagcccggcg ccaagatcaa agcattccag aaagaggacg attccgcgat gatgcggttc
      361 gaggtggctt tcggcaaggc ctacgccgcg ctcgcactgg gccgctcgtc gtcattggtg
      421 cgcatgcgca ccgaagaacg gccgctgttc atgcagtatc taatccgcgc ttccgacgac
      481 cagatttttc ccgagggcgg cggcatgctg attcgcgatc ccgatggcga actgctcggc
      541 gccgtcggcg tcaccggcga tactcaggag cgcgacgagg aactcgccgc ccacggcatc
      601 cgcgccgctg gactcaaaac cgacgaagac tgcgcccaat tcggctcgaa ggtgcgactg
      661 aagaactgag gcattaggca atgggcatta gcgttaggca atgggggaga aagaaaactc
      721 ttgcctaatg cctagcgcct tctcatgcaa tccactccga gaccggagct ttggcttcct
      781 cgagcggctg gcagacgata tcgactaaac tgggaccgcc gtacgccagt gcttctttca
      841 gcgtcatcgc aacgtttacg gggtcggtca cacgccagct tttgacgccg aaggcgctgg
      901 cgacggcggc gtgatcggtc gcagcgaaat ccaccgagaa gtaacgtttg ccgtagccgg
      961 acttctgacc ggctttgatc cagccgtagc tggcgtttgc aatcaccaca aaagtaatcg
     1021 gcaggcaata ccgcgcggcg gtctcgagct cgccgcagct aaatccaaag ctgccatcgc
     1081 ccatcaccga gacgcatttt actgcagtct tggcgaggtg agcgccgatc gcggcggaca
     1141 gcgagtaacc gagagccccg tgagcacgat tcgaaaagaa ccgccgaccg ctcccgccca
     1201 actcgtaata agcagacaag tagggacatg gcgtgccggc gtcggcgacg acgatggcat
     1261 cgggatcgag cgcgccggca agcgcggcca cgacgcgctc aggcttgatt ggtttttccg
     1321 ccgattccgc gagcgcttgg aacttggcga atttgtgctc tttggcgttt tgcacgagtg
     1381 cagcatcgag tggccgccgc aggtctccga gcgcttcatc caacattctc agtgccagtt
     1441 tggcgtcgcc aacaacggcg gcgtcggttg gataattcgt tccgggcact gcggagtcga
     1501 cgtcgagatg aacgattttc acctggcctg gaacgggatg gcgccagcgt tcagtcgtca
     1561 ccgagccggc gcggcagcca acgaaaatga ccaggtccgc ttgatctacg atggaccgag
     1621 tttcgggtgt cccgccgttg gagccgacta cgccggccgc aagcggatgc tgctcgtcga
     1681 tggagccttt gccgctgatg gtcgtcgcta ccggagccga gatccgctcg gcaagggcga
     1741 gcagttccgg ctccgctccg gagacaacga caccgccgcc gcaaataaaa accggccggg
     1801 ccgccgcgcg caacagccga gcagcgcgtt cgatcgaatc gacttcgggc gcactgcgtt
     1861 cggccggaca gctgccgaac tttgtctcgc cccaaacttc agtgcgattc actaaaccat
     1921 tttgtacgtc gaaggggaga gcgatatgcg ccgcaccggg acggccggtg gtgctttgcc
     1981 gaaacgcctg gcggaaaacc gccggtatat cttcggcgcg atcgagaaca gtgttccact
     2041 tcgttatcag ccgcatcagc gcgccttgat ccaatgccgt caaggtatag cggccgcgcg
     2101 cggcgacgga aatatccgaa ttgatgcaga gcagcggcac ggaagattca ttggcttcag
     2161 ccaacccggg aacgatgtac gtcacgccgc cgccgctcgg gccctcgcac accccgactt
     2221 tcttcgatac tcgcgcatag ccgtccgcca tgtacgcggc ggagcgctcg tcacgtgcga
     2281 ggatgtgacg catgccatgc ggcaggcgcg cgagcgcgtc gtaaaacggc aaactggtat
     2341 cgccgcatag gccgaagatc aattcgactt cgtgggcttg cagcatctca accactgctt
     2401 cggcgccggt gatggttgaa gacggtggtt gggcaggctt gttcatagaa tgtttcatgc
     2461 tttgatctat tctaccgtgc tctcacgcca tacgttgctc acttcgttgc gcggaaaaaa
     2521 acgcgcgatc ggcgagctgt ctttcaccgc tggcgccgat cgtattatga tggtctagag
     2581 aaacacaaac agtggcgatc aacggat
//
LOCUS       DASULZ010000184         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_630698, whole genome shotgun sequence.
ACCESSION   DASULZ010000184 DASULZ010000000
VERSION     DASULZ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630698"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VFY96_03755"
     CDS             <1..674
                     /locus_tag="VFY96_03755"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX5605603.1"
                     /translation="QGNTTNGVGIDKASLSMSLYHVLDGSRVMGDVVCETELPGLLLV
                     PATKDLVGAEIELVAGDRREYRLKDALASVTAHYDYVLLDCPPSLGLLTLNALTAADS
                     LLIPIQSEYYALEGLSSILETMRLVQQSFNLTLRLEGIVITMFDSRNRLSHQVVEEIR
                     AHFPDKLFHTIIRRNVRLSESPSYGKPICIYDAGSTGAQDYRELAKELTNRGEHNVDA
                     EKRLG"
     gene            646..1527
                     /locus_tag="VFY96_03760"
     CDS             646..1527
                     /locus_tag="VFY96_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX5605604.1"
                     /translation="MSMPKKGLGRGLSALIPSATEPKADSRANSAPLEVSIDRITPSP
                     FQPRRSFDEAKIAELAGSILNQGIIQPLVVRPKADGFELIAGERRWRAAMKAGLSRVP
                     VVIRDASDHEALQLALVENLQREDLNPIEEANGYRRLQEEFHWSQEEIAEKVAKSRPA
                     ITNSLRLLSLPVEVQQEVSAGNLPAGQARALLGLHTEALMISACREVIAKGLSTRETE
                     KMVRHLIAGRKRRRAPLATDPDLKTIIEELQRVLGTRVRLIPKPRTNKGRIEIEYYTL
                     PDLERIIQTITRQTGTA"
     gene            complement(1801..>2062)
                     /locus_tag="VFY96_03765"
     CDS             complement(1801..>2062)
                     /locus_tag="VFY96_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX5605605.1"
                     /translation="KEIVIVGDRSDARTADLIKEIHSVYLPNKAVQLFAPGEPLEKVS
                     PLMTGKQQIDGRPTAYVCQNFTCSAPVTSWAELKPLLKTDAA"
BASE COUNT          468 a          594 c          587 g          413 t
ORIGIN      
        1 cgcagggcaa taccacgaac ggggtgggta tcgataaggc gtcactttcg atgagcctct
       61 atcacgtgct cgacggatcg cgagttatgg gcgacgttgt ctgtgagacc gagctaccgg
      121 ggcttttact agttcctgca accaaagacc ttgtcggtgc ggaaatcgaa ctggtggccg
      181 gggatcggcg cgagtaccgt ttgaaggatg cgcttgccag cgtgacagcg cattacgact
      241 acgttctcct cgactgtccg ccgtccttgg gactgctgac gctcaacgcc ttgactgccg
      301 cagactcgct gctgattccc attcaaagcg aatattatgc tttggaaggc ttgagctcga
      361 tcctggaaac catgcggctg gtgcagcaga gcttcaatct aacacttcgc ttggaaggaa
      421 tcgtgattac gatgttcgac agccgtaatc gcctatcaca ccaggtggtg gaagaaattc
      481 gcgcccactt tcccgacaaa ctatttcaca cgatcattcg ccgcaacgtg cgcttgagcg
      541 agagcccaag ctacggcaag cccatctgta tctatgatgc cggatccacc ggtgcccagg
      601 attatcgtga acttgcaaag gagttgacca accgtggaga gcacaatgtc gatgccgaaa
      661 aaaggcttgg gtagagggct gagcgcgctc atcccgagcg ccaccgagcc caaagctgat
      721 tcgcgcgcta attccgcgcc cctcgaagtt tcgatcgacc gcatcacgcc aagccctttc
      781 cagccacgcc ggtccttcga cgaggcgaaa atcgccgagc ttgccggctc gattctgaac
      841 caggggatca ttcagccgct ggtggtccgt cccaaggctg atgggttcga gcttatcgcg
      901 ggcgagcgcc gctggcgcgc cgctatgaag gcggggctga gccgcgtgcc ggtagtgata
      961 cgagacgcga gcgatcatga agctctgcaa ctcgcgctgg tcgaaaacct gcaacgcgag
     1021 gatttaaatc cgatcgagga agccaacggc tatcggcggc tccaggagga gtttcactgg
     1081 agtcaggagg agatcgccga aaaagtcgcc aagagccggc cagcgatcac caattcgctc
     1141 agactgctgt ctctgcccgt cgaggtgcag caggaagttt cggctgggaa cctgccggcc
     1201 ggtcaagcgc gcgcgctgct tggcttgcac acagaggcac tgatgatttc cgcctgccga
     1261 gaagtcatcg ccaaaggcct ctccacgcgg gaaacggaaa aaatggttcg ccacctgatc
     1321 gcaggacgga aacgccggcg tgcgccgctc gcgaccgatc ccgatctgaa aacgattatc
     1381 gaagagctgc agcgtgtcct cggcacgcgc gttcggctga tccccaaacc gcgcacaaac
     1441 aaagggcgga tcgaaatcga atactacacg ttgccggatc tcgagcgaat tatacagacg
     1501 atcacgagac aaaccggtac cgcgtgaccc gaccgtacga agtttcgctc ctggtatgct
     1561 ctgggacaga cggggaagat ttctcccgtg aatcgaaatg acaagtacgc aatgctggtc
     1621 tgcagctcaa ccacctgagg ctcggaccgg ccggtaccgc gaaaaaatgt tttcttcagc
     1681 caggcagagg aaaggatctt accgcaatcg gtgaaaccgc caccgaatcc tctggatttc
     1741 cgcgcaattt atttcctgtg atggttttga gcgagcagta gaagatttga gtgccaaggg
     1801 ctaagccgca tcagttttca acagcggctt caactcggcc cagctcgtca ccggcgccga
     1861 acatgtaaag ttctggcaaa catatgcggt cggccggccg tcgatctgct gtttgcccgt
     1921 cattagcggc gagactttct ccagcggctc acccggcgca aaaagctgca cagctttatt
     1981 gggcaaataa accgagtgga tttccttgat gagatccgcc gtgcgcgcat cgcttctatc
     2041 accgacgatg acgatttcct tg
//
LOCUS       DASULZ010000185         3648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_529741, whole genome shotgun sequence.
ACCESSION   DASULZ010000185 DASULZ010000000
VERSION     DASULZ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529741"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            623..1108
                     /gene="rimP"
                     /locus_tag="VFY96_03770"
     CDS             623..1108
                     /gene="rimP"
                     /locus_tag="VFY96_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005370371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX5605606.1"
                     /translation="MKQAPEHLVSLIEPVVEGLGYECVGIEYHPHPKHGLLRIYIDSD
                     NGILVDDCSKVSHQVSGVLDVEDPIPDNYQLEVSSPGADRPFFKISQFERFKGSTAQV
                     SLFKAIGGRKRITGLIEKVEEDIITLIEHGQTFEVPFSAISKARLVPDYLIEKGVRSG
                     K"
     gene            1098..2612
                     /gene="nusA"
                     /locus_tag="VFY96_03775"
     CDS             1098..2612
                     /gene="nusA"
                     /locus_tag="VFY96_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005370372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX5605607.1"
                     /translation="MANKEILLVVDVFSNEKAIEKEVVFQAIESALEMATIKRYANPI
                     KARVEIDRKTGDYKTYRLWDVVAPNSEINGDVEFPGTQILLDVAKFDNPDAQVGDVVE
                     EEIESVEFGRIAAQTAKQVIIHKVREAERKKIVDAYKSRVGELITGVVKRIEKGSVYL
                     DLGGHVEAYIAKEDMIPREAVRVGDRIRGYLKDVRSEPRGPQLFVSRTAPELLIALFR
                     LEVPEVSEGLISLMGAARDPGSRAKIAVKSNDPRLDPVGACVGMRGSRVQSVSNELAG
                     ERVDIILWNPNEAQYVINAMSPAEIQSIVVDEDRHSMDLAVSTDNLSQAIGRGGQNVR
                     LATLLTGWELNVIDASKAEANSEAEANKIKDIFVKELDVDDDIALTLVEVGFNTIEEI
                     AYVPVSEMLEIEGFDEELVNELRSRAKDSLLISAIAAEEKIETNEPAQDLLEMEGMDK
                     ELAYELASRGIITMDDLAEQSIDDLLSLSEMDEDRAAKLIMKAREPWFSEEKGE"
     gene            2627..>3648
                     /locus_tag="VFY96_03780"
     CDS             2627..>3648
                     /locus_tag="VFY96_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005370373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HEX5605608.1"
                     /translation="MSDQTVQQLAEVVKIPLERLLEQLKEAGLSANTPDDVISEDEKM
                     QLLAHLRKRHGKDEGEAKTAPRRVTLERRKVIEIKQASVPGTATKTISVEVRKKKTYI
                     NKRELAETAETKTAAGQPSVHEEEPIIKISEPVIAAEQKEKPIEAVQVTEAAIEAAPP
                     VEAKKMPAAGQPEEYEYEEVAVQAKAPVEEELAPVTRFETVKAKETKKVKNEKVGKVK
                     EADEVKKKITDKDRRVEESINRTAEKVRALALQKQETLHRKKQRDDSPFSEGRDAPRR
                     PKKKGKAPQQQRSNAGFEGKHQFEMPSAPIVKEVTIPETILVSDLANKMSVKVALVIK
                     QLMKLG"
BASE COUNT         1001 a          807 c         1086 g          754 t
ORIGIN      
        1 acccttgtgg tggagcttgt cgaatcataa atgcttaacg gaattgatgg agtcactgcc
       61 ccgtaggtaa ggtattggaa caaatgcggt tgactgggtg aaagcgaaat tcgtgatggc
      121 ttgttctgaa tttctttaac tgcaatgggg aatgagaaag gttgttcgcg gtacgattta
      181 acaaacgggc aaaaatagca gatatgccct ccctgctcga tgtggcgcct ttgatatgtg
      241 ccctgatgag aaaaccgcag aatgccgtaa acagctgaca agccttggaa gaagccaaaa
      301 aaccgggaaa agggaacctc gctgccggca attttagact tgaattgagt agggatgtat
      361 tttgaaggat caaagcgctt tgaaaatgac taagctttgt gctatattag cttttttagg
      421 atgtatcgac acattacggc gttgttatcc taacggcagg ggctcaaagg ttgttggttc
      481 aaaggtcccc gctgccattt acacggaagc cccatcatgg ggttttttat tttctggctc
      541 cgtgtttgtg gctgcagcat tggctagtga atgataagag ccctgggctc tttttttttg
      601 tgcggtgaaa aagcgaggaa aaatgaagca ggctcccgaa catttagtct ccttgatcga
      661 gccggtcgtg gagggcttgg gttatgaatg tgtcggcatt gaatatcacc cgcatcccaa
      721 acatggttta ttaaggattt atatcgacag cgacaacggt attctggtcg atgactgctc
      781 gaaagtcagt caccaggtca gcggcgtgct ggatgtcgaa gatccgattc cggacaatta
      841 tcaattggaa gtatcctcgc cgggggcgga ccggccgttt ttcaagatta gtcagtttga
      901 acgcttcaag ggcagcaccg cgcaggtgag tctgttcaaa gcgatcggcg gacgtaaacg
      961 gatcaccggc ctgatcgaaa aagtcgagga agatatcatt acactgatcg agcacggaca
     1021 aaccttcgaa gtgccgtttt cggcgatcag taaggcgcgt ctggtgccgg attatttaat
     1081 cgaaaaagga gtacgcagtg gcaaataaag agattttgtt ggtcgtggat gtcttttcca
     1141 acgagaaagc gatcgaaaaa gaagttgtct tccaggcgat tgaatcggcg ctggaaatgg
     1201 cgacgatcaa gcgctatgcc aacccgatca aagcccgcgt cgaaatcgat agaaaaaccg
     1261 gggattataa gacttaccgt ttgtgggacg tggttgcgcc gaacagcgaa atcaacggcg
     1321 atgtcgagtt tccgggcacg cagattttgc tcgacgtggc gaagttcgac aatccggatg
     1381 cccaagtcgg cgatgtggtt gaggaagaga tcgaatcggt cgagttcggc cgtattgcgg
     1441 cacagacggc gaagcaggtg atcatccaca aggttcgcga ggctgagcgc aagaaaattg
     1501 tcgatgccta caagagccgc gttggcgagt taattaccgg cgtcgtcaag cgtatcgaaa
     1561 aaggcagcgt ctacctggac cttggcggac atgtcgaagc ctatatcgcg aaggaagaca
     1621 tgattccgcg agaagcggtg cgtgtcggcg atcgtatccg cggctatttg aaagatgtgc
     1681 gctcggagcc gcgcggcccg caattgtttg tcagccggac ggcgccggaa ttgctgatcg
     1741 ccttgttccg gctggaagtg cctgaagtca gtgaaggttt gatttcgttg atgggcgcag
     1801 cccgcgatcc ggggtcgcgt gcgaaaattg cagtcaagag caacgatccc cgtctcgatc
     1861 cggtcggcgc ctgtgtcggc atgcgcgggt cgcgtgtgca atcggtttcg aacgaactgg
     1921 caggcgaacg ggtcgatatc attctgtgga atccgaacga agcgcaatat gtgatcaacg
     1981 cgatgtcgcc ggctgaaatt cagtcgattg tcgtcgatga agaccggcac tccatggatc
     2041 tcgcggtcag caccgacaat ctgtcgcagg cgatcggccg cggcggccaa aacgtgcgtt
     2101 tggcgacgct attgaccggt tgggaactga acgtgatcga tgcatcgaaa gccgaagcga
     2161 acagcgaggc ggaagcgaac aagatcaagg atatattcgt caaggaactc gatgtcgatg
     2221 acgacattgc gttaaccctg gtggaagtcg gttttaatac gattgaagag atcgcttatg
     2281 tgccggtcag tgaaatgctc gagatcgaag gcttcgacga ggaattggtg aacgaattac
     2341 gcagccgcgc gaaggattcc ttgttgatca gtgcgattgc ggccgaggaa aaaatagaaa
     2401 ccaacgagcc agcccaggat cttttggaga tggagggtat ggacaaggaa ctggcttatg
     2461 aattggccag ccgcggtata attacgatgg atgatctggc ggaacagtcg atcgacgatt
     2521 tgctgagctt atccgaaatg gatgaggatc gggcggcaaa gttgattatg aaggcgcgag
     2581 agccctggtt ttctgaagaa aaaggcgagt aaggcgggag taggcgatga gcgatcaaac
     2641 agtacagcaa ttggccgagg tagtcaaaat acctctggaa aggttattgg agcagttgaa
     2701 ggaagcgggt ttatcggcca atacgccgga cgacgtgatc agcgaagacg aaaaaatgca
     2761 gcttctggcg catttgcgca aacgccacgg caaggatgaa ggcgaagcga aaaccgcgcc
     2821 tcggcgcgtg acgctggaac gccgcaaggt gatcgaaatc aagcaggcga gcgtgccggg
     2881 caccgcgacc aaaacgatca gcgtcgaagt tcgtaaaaag aagacctata tcaacaagcg
     2941 cgagcttgcc gaaacggctg aaacaaagac cgcagcaggg cagccttcag tccatgaaga
     3001 agaaccgatt atcaaaattt ctgaaccggt gattgcggcg gaacaaaaag aaaaaccgat
     3061 cgaagccgtt caggtgactg aagcggcgat agaagcggcg cctccggtgg aagccaaaaa
     3121 gatgccggcg gcgggacaac cggaagagta tgaatatgaa gaagtagccg ttcaagctaa
     3181 ggcaccggtc gaagaggagc tggcgccggt aacccggttt gaaacggtca aagccaagga
     3241 aaccaaaaaa gtcaaaaatg aaaaagtcgg taaagttaag gaagccgatg aagtcaaaaa
     3301 gaaaataact gacaaggatc gccgggtcga ggaatccatc aatcgcaccg ccgaaaaagt
     3361 ccgcgcgctc gcattgcaaa aacaggaaac gctgcatcgc aaaaaacagc gcgatgacag
     3421 tccgttttcc gaaggccgtg acgccccgcg acgtccgaag aaaaagggca aggcgccgca
     3481 gcagcagcgt tcaaatgcgg gatttgaagg caagcaccag tttgaaatgc cgtccgcccc
     3541 gatcgttaaa gaagtcacga tccccgaaac gattctggtt tccgatctcg cgaataagat
     3601 gagcgtgaaa gtagcgctgg tgatcaagca attgatgaag ctcggcat
//
LOCUS       DASULZ010000186         4869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_326717, whole genome shotgun sequence.
ACCESSION   DASULZ010000186 DASULZ010000000
VERSION     DASULZ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326717"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VFY96_03785"
     CDS             complement(<1..257)
                     /locus_tag="VFY96_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605609.1"
                     /translation="MMRKSRKMLAANSELRSDPQLRMLAFGLICAFGVMAVSGCTTSK
                     ETRLPPAAQAPAVPQPASGSAAATATPMQSLQELSVREEKG"
     gene            complement(260..868)
                     /locus_tag="VFY96_03790"
     CDS             complement(260..868)
                     /locus_tag="VFY96_03790"
                     /inference="COORDINATES: protein motif:HMM:NF016254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEX5605610.1"
                     /translation="MHSSSTIIVVVCLSLAALSHGAEEPMPSDRMKQAIGKLNKAPAS
                     IGQSLQGLTDAAATKLKQLSGSDNPAPQSNAIDMTVPQKKTDIAPPAHVMKPDGRDPF
                     RPMALKTNAASRSRENLSPLERFDIGQLKIVGIVSDVKEPRAMVEDSAGLGYTVKVGT
                     PIGSNDGRVKVIGRDQIVIEEVYVDAYGARKKRDIAMRLSTE"
     gene            complement(875..1498)
                     /gene="pilO"
                     /locus_tag="VFY96_03795"
     CDS             complement(875..1498)
                     /gene="pilO"
                     /locus_tag="VFY96_03795"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEX5605611.1"
                     /translation="MNQILDAILERSTAQKIAILAVTVILMVALYYSFLFGPKADELA
                     KLADSVEIARNEKTVKTQKAANLSRLRQDLQRLDAELRKAIAQLPEKKQIPELLSSIS
                     SKAQQSGLDVLLFRPRAESYQEFYAEVPVDITVKGNFYNTVNFFDEVGRMDRLVNIDN
                     IGFKNPTVSGENVVLETTSVATAFRFLDEAERKKVAEEKAKAAKTKK"
     gene            complement(1495..2106)
                     /locus_tag="VFY96_03800"
     CDS             complement(1495..2106)
                     /locus_tag="VFY96_03800"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEX5605612.1"
                     /translation="MIRVNLLPIKALQAEVTRRREIIIGSSVLGSLAALLIGAHIYQA
                     HQLSQLQNELSALRTELQTLTLKVKEVGDLQNKIKDFRSKNKIIEDLNRKKSGPVLVM
                     ESLSSAVPTSLWLTEFKETAGSLTMNGLAVDNQTIAEFMKAIAQSKYFKDVELVETTQ
                     GAGPSAALKKFALKTTIVYRPPESIPVSSKPAPAVAVKKEEMH"
     gene            complement(2103..3203)
                     /gene="pilM"
                     /locus_tag="VFY96_03805"
     CDS             complement(2103..3203)
                     /gene="pilM"
                     /locus_tag="VFY96_03805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEX5605613.1"
                     /translation="MLPFLKNFNLGWLNIFAANDGYVALDIGSSSVKMIEAAVDRNGY
                     RILSLGILPLPECAIQNNMVVDSKPVVESIRKLISDNRVKSTQVISAVPGRAVIMKKV
                     QMPKQEPAELEANIEFEAQNVIPENLENVNLDHQVLQEIDGGNKMDVLLVAVKKEIVN
                     SYADAIEEAGLIPTIMDVDYFALENMYEANYAAEAEHGVTGIIHIGAQYTSITLLQNG
                     ISIFTGDLSVGGAYFTDNLARQLGVSVEAAETFKTTGILEGNKGLDLESTLTATSEEL
                     ADEIRRTVSLYGAFPSDDGDGLKVIYISGGGAKLAGLRTLLEDRMAVPVRLSEPFRRF
                     TVNKNIDRTFLTEAAPYFAVGAGLSIRRAGDK"
     gene            complement(3205..3405)
                     /locus_tag="VFY96_03810"
     CDS             complement(3205..3405)
                     /locus_tag="VFY96_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5605614.1"
                     /translation="MDHENSVKKMREAKMLSKTELARLAGISLLTLNRIEEGKDCRMA
                     TKRKILLALGLSIEQKQTLFKR"
     gene            3708..>4869
                     /gene="priA"
                     /locus_tag="VFY96_03815"
     CDS             3708..>4869
                     /gene="priA"
                     /locus_tag="VFY96_03815"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX5605615.1"
                     /translation="MKSARQFARVVVPGPFKEPLIYGVPESLQDRVVPGLRVLVPLQK
                     RTVTGIFLESVRENLLHQTKDIIELLDEQPFLDGHLLQLAKWIAQYYVASLGEVVASM
                     LPPNSRRQSQRMVVLKQAEAKITDELGRKILRILRQHKGKMPSAALKRAFPEAALQTV
                     LAQFESRGIIEINDHLPRQRTSKDQSWPVAGAPATDAPTKFRLTVEQNSAVRTIEERI
                     SSGGFETFLLHGVTGSGKTEVYLRAMERTKALGRRSLILIPEISLTPQLLDRLRARFP
                     NNTGVLHSALTAAERLAQWRHIAQGNVDVVIGARSAVFAPIPDLGLIVVDEEHDPSYK
                     QEETLRYNGRDVAVVRGKLLACPVILGSATPALETYENCSQGRYRLLEITRRV"
BASE COUNT         1048 a         1357 c         1253 g         1211 t
ORIGIN      
        1 tgtccctttt cttcgcgcac actcagctct tgcaggctct gcatcggagt cgcggttgct
       61 gcagcgctgc cgctggccgg ctgtggcacg gccggcgctt gtgccgccgg tgggagccgc
      121 gtttctttgc tcgtcgtgca gccgctgaca gccattaccc cgaaggcgca aatcaatccg
      181 aatgcgagca ttcggagttg cggatcgctc cgcaattcac tattcgcagc aagcatcttc
      241 ctgcttttcc tcatcatcgc tactccgttg agagtcgcat cgcgatatcg cgttttttac
      301 gggctccata agcgtccaca tagacttcct cgataacgat ctggtcccgt ccaattactt
      361 ttacccgacc gtcgttactg ccaatcggag ttccaacttt gacggtgtag ccaagtccgg
      421 cgctgtcctc gaccatggca cgcggttcct taacgtccga cacaatcccc acgatcttga
      481 gctgcccgat gtcgaaccgc tcgagcggcg aaagattctc gcgtgagcgc gacgccgcgt
      541 tggttttgag cgccatcgga cggaatggat cgcggccatc cggtttcatt acgtgcgctg
      601 gcggtgcaat gtcggttttt ttttgcggca cggtcatgtc gatcgcgttg gactgcggcg
      661 ccgggttatc actgccggaa agctgcttca gtttcgtcgc cgccgcgtcc gttaacccct
      721 gtaaactttg gccaatgctc gccggtgcct tgttcaactt gccgatcgct tgtttcatcc
      781 tgtccgacgg catcggttct tcggcaccgt ggctcaacgc ggctaggctg aggcaaacga
      841 caacgatgat ggtactggag gagtgcatcg atctctattt cttcgttttg gccgctttcg
      901 ccttctcttc ggcaactttc tttcgctccg cctcatcgag aaaacgaaac gccgtcgcga
      961 cactggtggt ctccaacacc acgttctcgc cacttacggt aggattcttg aagccgatat
     1021 tgtcgatatt gaccagtcga tccattcgtc caacttcatc aaaaaaattc accgtgttat
     1081 agaaattacc cttgaccgta atgtccaccg gtacttccgc gtaaaattct tggtagctct
     1141 ccgcgcgcgg acgaaataga aggacatcga ggcccgactg ttgggctttg ctggaaatgc
     1201 tgctaagcaa ttccgggatt tgcttcttct cgggcagctg agcgatcgcc tttcttagct
     1261 cggcatcgag acgttgcaga tcttgacgca aacgcgacaa gttggctgct ttttgggtct
     1321 tgaccgtttt ctcgttgcgc gcaatttcca cactatcggc caatttcgcc agctcgtccg
     1381 ctttcgggcc aaacaaaaag ctgtaataca gcgcgaccat taaaatcaca gtcaccgcga
     1441 gtatcgcgat cttttgcgct gtcgatcgct ctaggatagc gtcgaggatc tgattcaatg
     1501 catctcctct tttttgacgg caacagccgg cgcgggcttg ctgctcaccg gaatggattc
     1561 gggcgggcgg tagacgatcg ttgttttgag cgcgaatttt ttcaatgcag cgctcggacc
     1621 agcaccttgg gtggtttcca ccagctcgac atccttgaaa tattttgatt gcgcgatcgc
     1681 cttcatgaac tcggcgatag tttgattgtc aacggcaagc ccattcatcg tcaggctgcc
     1741 ggcagtttct ttgaactccg ttaaccaaag actcgtggga actgcgcttg aaagactttc
     1801 catgaccaat accggaccgc tcttctttct gttcaggtct tcgatgattt tgtttttgct
     1861 acggaaatct tttattttgt tctggaggtc gccaacctct ttaaccttca aagtcaaagt
     1921 ctggagctcg gtgcgcaacg cggaaagttc gttttgcagt tgcgagagct gatgagcttg
     1981 ataaatatgc gcgccgatca aaagggccgc taaagagccc agcactgagg aaccgatgat
     2041 gatctcgcgc cggcgcgtga cctccgcttg caacgcttta atcgggagta ggttgactcg
     2101 aatcatttgt ctcccgcgcg gcgaattgac aatccagcgc ccacggcaaa atagggggcc
     2161 gcttccgtta aaaacgtccg atcgatgttt ttgttaaccg tgaagcggcg gaacggctcg
     2221 ctcaagcgca ccggcacagc catccgatcc tcgagtaacg ttcgtaagcc ggcgagcttg
     2281 gcgccgccac cgctgatata gatcaccttg agaccatcgc catcgtcgga cggaaacgcc
     2341 ccgtagaggc ttaccgttct cctgatctca tcggccagct cttcggatgt cgctgttagc
     2401 gttgattcca aatctaaccc cttgttgccc tccaaaatgc ccgttgtttt aaaagtctca
     2461 gcggcctcaa cagacacccc gagctgcctt gccagattat cggtaaagta cgctccgcct
     2521 accgacaagt cgccggtaaa aatcgaaatc ccgttttgca gaagtgtaat gctcgtatac
     2581 tgcgcgccga tatgaatgat tccggtgaca ccgtgttcgg cttcagccgc gtaattggct
     2641 tcgtacatat tttccaaggc aaagtaatca acgtccatga tcgtggggat caatccggct
     2701 tcttcgatcg catcggcata gctgttgacg atttcctttt tcaccgccac caacaagaca
     2761 tccattttat tgccgccatc gatttcctgc agcacttgat gatccagatt gacgttctcg
     2821 agattttccg gaatgacatt ttgcgcttca aattcgatat tggcttctag ttccgcgggc
     2881 tcttgtttcg gcatctgcac tttcttcata attacggccc ggccgggcac ggcagaaatc
     2941 acctgtgtgg atttaactcg gttatcactg atgagcttgc gaatactttc caccaccggt
     3001 ttggagtcca cgaccatgtt gttctgaatc gcacactcgg gcagcggcag aataccgagg
     3061 ctgaggattc gataaccatt tcgatcaacc gcagcttcga tcatcttgac cgaactcgag
     3121 ccgatatcca gcgccacgta gccatcgttg gcggcaaaga tgttgagcca acccaaatta
     3181 aaattcttca gaaaagggag catcttagcg cttgaacaga gtttgctttt gctcgatcga
     3241 aagccccaga gccaagagaa tcttgcgctt agtcgccatg cgacaatctt tgccctcttc
     3301 gattcgattg agagtgagta gcgaaatgcc cgcgagccga gctaactcgg tcttgctgag
     3361 catcttcgct tcacgcatct tcttgacgct gttctcgtga tccatcccgc tgatcccatc
     3421 cacagtctag acgcgcagaa gtttccgagg cttttataaa ccagctccgg ttaagtaaaa
     3481 cttgccttaa ttatgccgaa ttgtggtaag tcgttgcgct cttaaagcaa actaactgcc
     3541 acgagcgcct gcgggatgct cgatcgttta cgcagtcaaa tcatggatat ccgaggcttg
     3601 ataaagccat gccataccca ccttgctccg ttgaacgaca gaattgaaca gaaacgttcg
     3661 gtgggcgcta agttgctttt ccaaaaaccc gtcggtagaa atcagcgatg aaatccgcgc
     3721 ggcaattcgc ccgagtcgtc gtaccggggc cgttcaaaga gccgctgatc tacggcgtgc
     3781 ctgaatcgtt gcaggaccga gttgttcccg ggctgagagt cttggtgcct ctgcaaaaaa
     3841 ggactgtgac cgggattttt ttggagtcgg tccgggaaaa cttgttgcat cagaccaaag
     3901 acattattga gttgctcgat gagcagcctt ttcttgacgg tcatttgctc cagctcgcta
     3961 aatggatagc gcagtactat gtcgcatccc tgggcgaagt agtcgcctct atgctgccgc
     4021 caaattcgcg ccggcaaagt caaagaatgg tcgtgctcaa gcaagctgag gcaaaaatta
     4081 ccgacgagct tggccgcaag attcttcgta tcttacgtca gcataaaggc aaaatgccga
     4141 gcgccgcgct caagcgggcg tttcccgaag cagcgctcca aaccgtactc gcccagtttg
     4201 agtctcgtgg aataatcgag atcaacgatc atctgccaag gcaacgaacc agcaaagatc
     4261 agagttggcc ggtcgcgggt gcaccggcga cggatgcgcc tacaaaattt cgcctgacag
     4321 tcgagcagaa tagcgcggtt cgcaccatag aggagcgtat cagcagcggc ggattcgaaa
     4381 cttttttgct gcacggtgtc accggtagcg gtaagaccga agtttacttg cgcgctatgg
     4441 aacggaccaa agcgcttggc cgacgaagtc tcattttgat cccggagatt tcactcacgc
     4501 cgcagctctt ggatcggttg agagcccgct tcccgaacaa caccggtgtg ctgcacagcg
     4561 cgcttacggc cgctgagcga ttggcccaat ggcgccatat cgcacagggg aatgtcgatg
     4621 ttgtcatcgg cgcgagatcg gccgtgtttg cgccgattcc tgatctagga ctgatcgtgg
     4681 tggacgaaga acacgacccg tcctacaagc aggaggagac gcttcggtat aatggcagag
     4741 acgttgcggt agttcgcggc aaactgttgg catgcccggt gattctgggc tcagccacac
     4801 cagcgctgga gacgtatgaa aactgcagcc aagggcgtta ccggctcctc gaaatcaccc
     4861 ggcgagtgg
//
LOCUS       DASULZ010000187         2555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_124133, whole genome shotgun sequence.
ACCESSION   DASULZ010000187 DASULZ010000000
VERSION     DASULZ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124133"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VFY96_03820"
     CDS             <1..261
                     /locus_tag="VFY96_03820"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEX5605616.1"
                     /translation="QRISLKKNRSRIGDIEEILIEGPSKLRNGQVMGRTRSNRIVNVT
                     GVEQFVGQLVPVRITGATATSLIGEACLNDSSLNSQQEGDMA"
     gene            258..785
                     /locus_tag="VFY96_03825"
     CDS             258..785
                     /locus_tag="VFY96_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEX5605617.1"
                     /translation="MMKKEDTIQMSVGGLTLDPVTKTPIVILKDTENKLNLPIWIGLL
                     EATAMATEIEGIKMARPMTHDLLKTILGEVGCSVECVEITELKENTYYAAVNLRVAGR
                     EMMIDSRPSDAIALALRTKSPIYVAKAVLEASSMLQQSEEGSERGVENVSNVSKEKWA
                     EILEKMGPEDFKYKQ"
     gene            862..1545
                     /locus_tag="VFY96_03830"
     CDS             862..1545
                     /locus_tag="VFY96_03830"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5605618.1"
                     /translation="MATASRRITRKQLRQPDKFQVFSDQALEYFAAHKALVFAAAGAV
                     IVLALALWGWQSFKERQNVAASHEFTRALALYQADKYGEAIPAFEKVQTYRWSRYAVP
                     AHLYLANSYLATNQLDKALNAAERSVVATKPETLYRQIALVTLGETQEQMKRCKPAIE
                     HFSEAQKIASALDSRATLGKARCAEALGDTKTALQAYKEYLKDNPDSPFAMKVAALEP
                     KSTSPTSTK"
     gene            complement(1878..>2555)
                     /locus_tag="VFY96_03835"
     CDS             complement(1878..>2555)
                     /locus_tag="VFY96_03835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF044548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEX5605619.1"
                     /translation="VSLGLQTRPDLIPGFAESSLHPWEMESALSLRDALANFSGGRVI
                     VGVPLGLYRCPPAPYEAQWMLDGYFREKGIRDRVQIEYFTRDPEPTGEAHDPVVWMDS
                     ESKRRGIKQNYEFVVRRIDPGRKLVQGLYNYNIPYDVLVMVPPHRPAQVLLDSGLADT
                     ETGIRVDYETLQTKWENVYAIGDCADMPASKAGGVAHQEADVLAHNLAVEITGKGRPV
                     DLWLHTI"
BASE COUNT          615 a          726 c          703 g          511 t
ORIGIN      
        1 cagcgaatct cgctgaaaaa aaaccggagc cgcattggcg acatcgaaga aatactcatc
       61 gagggcccgt cgaaactcag aaacggacag gtaatgggtc gcactcgcag caatcgcatc
      121 gtgaacgtga caggggtgga acaatttgtc ggccagctcg tgccggttcg aatcaccggc
      181 gccacggcaa cctcgttgat aggcgaggcg tgcttgaatg acagtagttt gaattcccag
      241 caagaagggg acatggcatg atgaaaaaag aagacacgat ccaaatgtcg gtcggcggtt
      301 tgactctcga tccagtcacg aaaacgccca tcgtgattct taaggatact gagaacaagc
      361 tcaatctgcc gatctggatc ggtctgttgg aagcgacggc gatggcgacg gaaatcgaag
      421 ggatcaaaat ggcacgtccg atgacccacg acttgctgaa gacgattctt ggagaagtcg
      481 gctgctccgt cgaatgcgtg gaaatcaccg agctcaagga aaatacttac tacgcggccg
      541 tcaatctgcg tgtcgcagga cgcgaaatga tgatcgattc acgcccgagc gacgcgatcg
      601 cgttagcctt acgtacgaaa agccctattt acgttgccaa ggccgtgctc gaggcgtcga
      661 gcatgctgca gcagagcgag gaaggaagtg agcgaggcgt ggagaacgtc tctaatgttt
      721 ccaaggaaaa atgggctgaa attctcgaga agatgggtcc ggaagacttt aaatacaagc
      781 agtaggtcgg agcgcgtccg gaaccctcac gatcaccgtt ccaaagttac aactcccgca
      841 gttcgataac caatcacatc catggccact gcgagccgac gaatcacgcg caagcaatta
      901 cgccagccgg acaagttcca ggttttctcc gatcaggcgc tggagtattt cgccgcgcat
      961 aaggccttag tgttcgcagc cgccggcgcg gtgattgttc tcgcgttggc actctggggt
     1021 tggcaatcct tcaaggaacg gcagaacgtc gctgcctcgc acgagtttac acgggcgctc
     1081 gcgttgtatc aggcagacaa gtacggcgaa gccattccgg ccttcgagaa ggtacaaact
     1141 taccgctggt cgcgctacgc cgtaccggcg catctttatc tcgccaatag ttatctcgcg
     1201 accaaccagc tcgataaggc attgaatgcc gctgaacgat cagtggtcgc gaccaagcca
     1261 gaaaccctgt atcgtcagat tgccctggtc acactgggcg agactcaaga gcaaatgaaa
     1321 cgatgcaaac cggccatcga gcatttcagc gaggcgcaaa aaatcgccag cgcattagac
     1381 tcgcgcgcga cactcggaaa ggcgcgttgc gccgaggccc tcggcgacac taaaacagct
     1441 cttcaagcct ataaggaata cctcaaagac aaccccgatt cgccgtttgc catgaaagta
     1501 gccgccctag agccgaaatc cacctcgccg acttccacaa agtaattccg aattctcact
     1561 tggcggcgag agcatagtcg ccctgctccg tcagaatcag caacgtctga taagatatat
     1621 tatgtcaact ttgctctgtg cgccggcgat gtagttcgca ccttaagtcg ccttctaccg
     1681 tgtgcatcgg ctctcgtctc acccggtatc caggatcaga agtaccgcca cagccaccat
     1741 ttctcgaaac cgatctttgc ccagtagatc atgcgcgccg ggccccaagt tcggggttcg
     1801 gcaactccga acggcggcca cccggtcgga taagtggttc gattggcagc ggcgcgtcca
     1861 gcgccgaacg ccagcagtca tatcgtatga agccagagat cgaccggtct tcctttaccg
     1921 gtgatctcaa cggccagatt gtgcgccagc acgtcggctt cttgatgggc cacgccgcca
     1981 gctttggatg ccggcatgtc ggcacagtcg ccgatcgcgt aaacgttctc ccattttgtc
     2041 tgtaacgttt cgtagtcgac cctgatgccg gtttcggtat cggccaaacc gctgtccaga
     2101 aggacttgcg ccggtcgatg cggcgggacc atgaccaaaa cgtcatacgg tatattgtag
     2161 ttgtacagtc cctgcactag ctttcgtccg ggatcgatgc ggcgcacgac gaattcatag
     2221 ttttgcttga tgccgcgccg cttgctctct gagtccatcc agactacggg atcatgcgcc
     2281 tcgccggtcg gttcggggtc gcgggtgaaa tattcgatct gaacccgatc gcgaatgcct
     2341 ttttcgcgaa aatagccatc gagcatccat tgggcctcgt agggggccgg cgggcatcga
     2401 tacagaccga gcggcacgcc gacgatgacc ctgccgccgc taaaattcgc caaagcgtcg
     2461 cgcagactca gcgcagattc catctcccac ggatgcagcg acgactcagc gaaaccggga
     2521 attagatcgg gtcgggtttg taaaccgaga gaaac
//
LOCUS       DASULZ010000188         3588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_326894, whole genome shotgun sequence.
ACCESSION   DASULZ010000188 DASULZ010000000
VERSION     DASULZ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326894"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="VFY96_03840"
     CDS             <1..873
                     /locus_tag="VFY96_03840"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX5605620.1"
                     /translation="AETIDEIADDLHLDGSGSELRMTQLPRASYAMPRIDADAKEARR
                     ADKFADELSEAPLHMSASGSRSSQRAVGTDLGSLWLLAKRLQKKSWAELSAVVASLVL
                     VIFGGMYLARPGSDTVSPPTDNIKSIQEGLGPISRELYRVLGRGASWETPVKQTRRSY
                     TDLEGREAYAHLGEAKTGTDRGKPGDSGPPINGSPRRDGSPMTETNRARLEHRNPQPP
                     RPGLSSSGFAVVGNSYVREKPASKAEVIATLRPGTRIQVVGRTGEYFRVRSLDHEAIR
                     GYVHKEDAFFEPYH"
     gene            938..2698
                     /locus_tag="VFY96_03845"
     CDS             938..2698
                     /locus_tag="VFY96_03845"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5605621.1"
                     /translation="MRENEWSRQGAEFLPLADPMYNEHFGFTESPFNVTPDSRFFFVN
                     PCYEEAFATLRYGIDGRKGFVVITGQPGTGKTTLLKRLTCSLDSNVQAACIFDPHLTF
                     IQLLRLTLGELGVASTGKDRLSMMGQLYDHLIQQLENGKIVCLMIDEAQNLSVEMLEE
                     LRLLSNLETDTDKLLQIVLVGQPEFEDRLERPELVQLKQRVALRCRLRPLELSEVSLY
                     LQSRLETIHYRRANLFDDESINLIALYSKGIPRLINSICDNALLIAYTTNRRYVIAQD
                     VREAAIELQLESIEPFSNQARLPLQQETKQNAGEKNGFERIFSPQSPLELTRAAADLP
                     SFQFPVEPDPGIGKPAQPTSAQPEFEPFVAYLGERSSRTRISGLYALGLSLVLIFAFG
                     GSALLYATRETQEWMPNFGGYLRELARLGHLPDGRRSELQSAENRLPGPQAPAAPSAD
                     DEKLENIPDRAPVKNDLASVPDNTADTEIQVTPEARDKAADRATTKIAEKRRAPEQRP
                     PRVRIANDQEIAGRKLEMEVYKAISDRAITGVEIVSVSDGTVYLNGRVATPRQKLAAV
                     RAALSVPGVRSVRDRIAIDN"
     gene            2951..>3588
                     /locus_tag="VFY96_03850"
     CDS             2951..>3588
                     /locus_tag="VFY96_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX5605622.1"
                     /translation="MPERDFYNILGLQRSASRDEIRKAYRKLARKYHPDINPGNKEAE
                     EKFKDVSVAYEVLSDPKKRKLYDEFGEAGLASGFDAEKARSYQQWRQQSSRAGASGAS
                     GGFSFNMDDLSDLFGGVGGFGGFGRGRAAAPTGPARGQDIESSMDIDFLDAVRGFQTA
                     ITLERPVECDACHGNGLQPGSKPTKCPECNGTGSVSVAQGPLQYRQTCPRCLG"
BASE COUNT          823 a          965 c         1047 g          753 t
ORIGIN      
        1 gcggagacca tcgatgagat tgcggatgat ttgcaccttg acggaagcgg ttcggaattg
       61 cgcatgacgc agctgccacg agcatcctat gccatgccgc gcatcgatgc ggacgcgaag
      121 gaagcgcgcc gtgcagataa attcgcagat gagctgagcg aggcgccgtt acatatgtct
      181 gcaagcggct cgaggtcaag ccagcgggcc gtcggcaccg atctgggttc gctctggctg
      241 ctcgccaagc gcttgcaaaa aaaatcatgg gccgagctat cggcggtcgt cgcaagcctt
      301 gtcttggtca ttttcggcgg catgtattta gcgcgaccag ggagtgatac cgtttccccg
      361 ccgaccgaca acatcaaatc aattcaggag ggcttagggc cgatctcgcg agagctttat
      421 cgcgtgctcg ggcgaggggc gtcatgggag acgccagtga agcagaccag gcgaagctac
      481 acggaccttg aggggcgtga ggcatacgct cacttgggcg aagccaaaac tggaaccgat
      541 cgaggaaagc caggtgactc cggcccgcca atcaatggtt ctccgcgacg cgacggctcc
      601 ccgatgacgg aaactaaccg tgcgaggctc gagcatcgaa acccacaacc gccgcgaccg
      661 ggactttctt cgtcgggttt cgccgtcgta ggaaactcgt acgtccgtga aaagccagcg
      721 tcgaaggccg aggtcattgc caccctgcgt ccgggtacac gaattcaagt agttgggcgg
      781 acgggcgaat atttccgtgt gcggtcgctc gatcacgagg cgattcgcgg gtacgttcac
      841 aaagaggacg cgtttttcga gccctatcat taaggccgcg cagtttgccg cgcgtaaaac
      901 gaaccgggct attgatacct gagccataga ttctaatatg agagagaacg agtggtcgcg
      961 ccagggcgcc gaatttttac cactcgctga tcctatgtat aacgagcatt tcggctttac
     1021 agaatcgcca tttaacgtga ctccggactc gcggtttttc ttcgttaatc cgtgctacga
     1081 agaggcattc gcgacgttgc ggtatgggat cgatgggcgt aaggggtttg tcgtcattac
     1141 cggtcaaccg gggaccggta aaaccacgct gctgaaacgc ttgacctgca gcctggattc
     1201 taacgttcag gccgcctgta tctttgaccc gcatctcacg ttcattcaac tgctgcgcct
     1261 cacgctcgga gaattggggg tggcgagcac cggtaaagat cgtctttcga tgatgggcca
     1321 gctttatgat catctgattc aacagctgga aaacgggaaa attgtttgct tgatgatcga
     1381 tgaagcgcaa aacctcagcg tggaaatgct cgaggagctt cggctcctgt caaacctgga
     1441 aaccgacacg gataaactgc tgcaaattgt tctcgtcggg caaccggaat tcgaggatcg
     1501 cttggagcgg ccggaattgg tgcaactcaa acaacgagta gcgctccgat gccggcttcg
     1561 gccgttggag ctaagtgaag tcagtttgta ccttcagtcg cgcctggaaa cgattcacta
     1621 taggcgagcc aatcttttcg acgatgaatc gatcaatctg attgccctgt attccaaagg
     1681 aattccacga ttgatcaatt cgatctgcga taacgcactg ctgattgcgt acacgaccaa
     1741 tcgtcgttat gtcatcgcgc aagacgttcg cgaagcagcg atcgagttgc agttggaatc
     1801 catagaacct ttttctaacc aagctcgttt gccgctgcag caggagacca aacaaaacgc
     1861 tggagagaaa aacggattcg agagaatatt ctctccgcaa tcgccattgg agttgacacg
     1921 agccgccgct gatttgccgt catttcaatt tccggtagag cctgatccag gcatcgggaa
     1981 gccggcgcag ccaacgagcg cgcagccgga gttcgagccc ttcgtcgcgt atctcggcga
     2041 gcgttcgtca cgaactcgca tcagcggctt gtatgctctg gggctgagtt tggtgctgat
     2101 ctttgcattt ggcggcagcg ctttgctgta tgcgacgcga gagacccaag agtggatgcc
     2161 gaatttcggg ggctacctgc gcgagctcgc acgattgggt catcttccgg acgggagacg
     2221 gagcgaacta caatccgccg aaaatcgatt acccgggccg caagctcctg ccgcgccatc
     2281 agcagatgat gaaaaactcg aaaatattcc ggaccgtgcg cccgtcaaga atgatctcgc
     2341 ctcggttccc gataataccg ccgataccga gatccaggtg actcccgaag cgcgcgacaa
     2401 agcagcagac cgagcgacga cgaagatcgc agaaaaaaga cgcgcgcctg agcagaggcc
     2461 gccgcgagtg cggatagcca atgatcaaga gatcgccgga agaaaactcg agatggaagt
     2521 gtacaaagcg attagcgatc gcgccattac cggggttgaa attgtgtcgg tcagtgacgg
     2581 cacggtttat ctcaatggca gggtggcaac accgcggcaa aagctcgctg cggtccgcgc
     2641 tgcgctgagt gtccccggcg tgaggagcgt gcgcgatcgc attgcaatcg acaattgatg
     2701 cgctatccga agccgttcac gcttcgctgc cccattctcc ttctcctgcc gtaacgaccc
     2761 ttccttcgta tctctagcac gacactgcgc cacggctccc ttggagcagt ggcagagctt
     2821 cgcttggggc atagaggaaa ttgacctgcc ggggcttctc tccttgcgga gcggcgcgat
     2881 ggaccttccg atgcggttgc ccggttcagt tttagcttga aagtctcaat agtgtggtta
     2941 ttctaaaggc atgccagaac gagattttta caacatccta ggtttacaac ggagcgcttc
     3001 gcgagatgag attcggaaag cgtatcgcaa gctggcgcga aagtatcatc cggatatcaa
     3061 tcctggcaat aaagaagccg aggaaaagtt caaagatgtt tcggtagcct atgaagtcct
     3121 gagcgatcca aaaaaacgca aactctatga cgagttcggg gaagcgggtc tcgcgtccgg
     3181 ctttgatgcc gaaaaagccc gcagctatca gcagtggcgc cagcaatcgt ctcgcgccgg
     3241 cgcctcaggc gcgtcaggcg gtttcagctt caacatggac gacctcagcg acttgttcgg
     3301 tggtgtggga ggattcggtg gtttcggcag aggcagagct gcagcgccaa caggtcctgc
     3361 tcgaggacag gacattgaat catcgatgga tatcgacttc ctcgatgcgg tgcgcggttt
     3421 tcagactgcg atcacgcttg aacggcctgt cgaatgcgac gcttgtcacg gcaatggatt
     3481 acagcccggc agcaagccca ccaagtgtcc tgaatgcaat ggtaccggta gcgtatccgt
     3541 cgctcagggg ccattgcaat accggcagac atgcccgcgt tgcttggg
//
LOCUS       DASULZ010000189         2357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_530530, whole genome shotgun sequence.
ACCESSION   DASULZ010000189 DASULZ010000000
VERSION     DASULZ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530530"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VFY96_03855"
     CDS             <1..327
                     /locus_tag="VFY96_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605623.1"
                     /translation="RPLAKAFLRAYGKAVHFLYTRQTESLAIFAKYTKIKDKKMLEGS
                     LKYGYEFMEKIPFVKRAGFQVTLDEIARTNPKAKQAKPEQFFDNSIIQELVDEGFFAK
                     LWGRNP"
     gene            406..1497
                     /locus_tag="VFY96_03860"
     CDS             406..1497
                     /locus_tag="VFY96_03860"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX5605624.1"
                     /translation="MPLFISNEEIDQLINMKDTMEALEILYREMGEGMAVTAPRSDVH
                     SPTAAGQSAEGPMAHYLKSMSGASPHFGTAALRFSSDIVAWRDSGGAMRREKLPMLPG
                     NRWMGIVLLFSSANGELLAIMNDGVLQRFRVGGANGVATKYLARQNAESVGLIGSGWQ
                     AGTQVMAVCQARPIKKIKVFSPTKANREKFAQETSERIGVEIVPVESYREAVKGVDII
                     VTSTNSRTPFLGKWVLAEGMHLSAMQRDEFDDAALLSCDPLVLHTHAIENNVTSSTLS
                     HFEREDFKLRDHPLDRGIDWKSLPTLPELVLGKIKGRQSDRQITGFVNNIGMGAQFAA
                     VGKKVYDAAKKSGAGREVPLDWFTQDVHP"
     gene            1781..>2357
                     /locus_tag="VFY96_03865"
     CDS             1781..>2357
                     /locus_tag="VFY96_03865"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605625.1"
                     /translation="MKQLRFVFVLALLLTAQETRAQQKFLMGYSSFSANQTSIWVAKE
                     EGFFKRFGTEPDLILIEGGTRGAQALISGDLPIMGMSGQPVISARARGSDLVMIAGTV
                     NKMNYVLVGAPTVKKPEDLKGKRIGAAQAGTASYHAVLLGLKHWGLDPRRDRITILQL
                     GNQGARVASLQSGGSDAIIVNPGLGPALKERG"
BASE COUNT          548 a          621 c          716 g          472 t
ORIGIN      
        1 cggccgctgg cgaaggcttt tttgcgggct tacggcaagg cggtccattt tctctatact
       61 cgccagaccg aatcattggc gattttcgcc aaatacacca agatcaaaga taagaagatg
      121 ctggagggaa gtctgaagta cggctacgag ttcatggaaa aaattccgtt cgtcaagcgc
      181 gccggatttc aggtcaccct ggatgagatc gctcgaacca atccgaaagc aaagcaggcg
      241 aagccggagc aatttttcga caacagcatt atccaggaat tggttgacga agggttcttc
      301 gcgaagcttt ggggacgaaa tccgtaagat tgccgctagc ggagaccggc ggtcgtttac
      361 aaagcccata ggttgaacgc aggcgagaaa taatccgagg tcggaatgcc cttattcatc
      421 agcaacgaag aaatcgacca gctgatcaac atgaaagaca ccatggaagc gctggagatt
      481 ctgtaccggg aaatgggtga aggcatggcg gtgaccgcgc cgcgctcgga cgtgcatagt
      541 cctactgctg ccgggcaaag cgctgaagga ccgatggcgc actatttgaa aagcatgagc
      601 ggcgcgagcc cgcacttcgg cacggctgcg ctgcgttttt cctccgatat cgttgcatgg
      661 cgggacagcg gcggcgcaat gcgccgcgag aagctgccga tgctccccgg aaaccgctgg
      721 atgggaatcg tcttgctttt cagctcagcg aatggcgagc tgctggcgat aatgaatgac
      781 ggcgtgctgc agcgcttccg tgtcggcggg gcaaacggtg tcgccacgaa atatttggcg
      841 cgtcaaaacg ccgaaagcgt cggtctgatt ggttcgggtt ggcaagccgg cacgcaagtg
      901 atggcggtct gccaagcgag acccatcaag aaaatcaaag ttttcagccc gaccaaagcc
      961 aatcgcgaga aatttgccca agaaaccagc gagcggatcg gtgtcgaaat cgtgcctgtc
     1021 gagtcgtatc gagaagcggt aaaaggcgtg gatattatcg tcacctcgac aaactcacgc
     1081 acgccttttc tcggtaaatg ggtgctggcg gaaggcatgc acctgagcgc gatgcagcgc
     1141 gacgaattcg atgacgcggc attgctcagc tgcgacccgc tggtgttgca cacgcatgcg
     1201 atcgagaaca atgtgacgtc gagcacgcta tcgcatttcg agcgcgaaga tttcaaattg
     1261 cgcgatcatc cgctggatcg cggcattgac tggaagtcct tgccgacatt gccggaactt
     1321 gtcctcggta aaatcaaagg gcgacagtcg gaccgtcaaa tcaccggctt cgtcaataac
     1381 atcggcatgg gagcgcaatt cgctgcggta gggaagaaag tctacgacgc cgcaaaaaaa
     1441 agcggcgccg gccgtgaagt gccgctggac tggttcaccc aggacgtgca tccgtagacg
     1501 acagttgccg gtcggtcatt cgatcgtccg cggctgaatc acagattcgt tgggcgggtg
     1561 gaggtgaaac gccgatacct gctgtcgggg catgatgggt atcgctggcg ctccacccat
     1621 cctcaagctc gagcctgtcc tgagcgcagc cgaaggatcg attccttcgc gcgcctcgtc
     1681 tctgaccgtt tttgagcagc ctgcactcgg agttttgagc ctgaggctta gacggatcgg
     1741 aatcaacgtt gaagacacga tcaagttgaa gggatccgcg atgaaacaac ttcgtttcgt
     1801 atttgttctt gcattactgc taaccgcaca agaaactcgg gcgcagcaga aatttctcat
     1861 gggctattct tcgttcagtg ccaatcagac gtcgatctgg gtggcgaagg aagaaggatt
     1921 cttcaagcgt tttggcacag agcccgattt gatcctgatc gaaggcggca cgcggggagc
     1981 gcaggcattg atttcgggcg acctgccgat tatgggcatg tcgggacagc cggtgatcag
     2041 cgcgcgtgca cgcggctcgg acttggtcat gatcgccggg acggtcaaca agatgaacta
     2101 tgttcttgtc ggcgcgccga cggtcaaaaa acctgaggat ctcaaaggca aacggatcgg
     2161 cgccgcgcag gcgggaaccg cttcgtatca tgcggtgctg ctcggcctta aacactgggg
     2221 cctcgatccg cgccgggatc ggatcacgat tctacagttg ggaaatcagg gcgctcgggt
     2281 cgcatcgctc caatcgggcg gcagcgacgc gatcatcgtc aaccccggtc tcggtccggc
     2341 cttgaaagag cgcggca
//
LOCUS       DASULZ010000190         2730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_936323, whole genome shotgun sequence.
ACCESSION   DASULZ010000190 DASULZ010000000
VERSION     DASULZ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936323"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VFY96_03870"
     CDS             <1..577
                     /locus_tag="VFY96_03870"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5605626.1"
                     /translation="AIAFIESHVVDYLNGGEAVSRENFPKGRIYSLLDCDGKPFMVHL
                     SGHNEAWEGLLKAGELQDLIGDSRFNMRKARTDRHDEIVTILREQFRRKPRDYWLPRL
                     DANHIPNAPINTIAEVFENPQIKHMGIPKQIAHPKLGTSNLVGSPINLSGTPARFYRA
                     APLLGEHNEEVLGKLGYDDQSLKELRASGII"
     gene            668..1882
                     /locus_tag="VFY96_03875"
     CDS             668..1882
                     /locus_tag="VFY96_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5605627.1"
                     /translation="MDFNVSADPPHVVEFRNEVAAWLAEQFKGYGHLRWSASWSTRED
                     DEEYKFRRGLAEKLGAKGWLFPIYPKKYGGAELTVDHQIVLETELFKYGLNLSHVFYT
                     LARIVAPVLIRWGTEEQKAEFLPPITRAQICVWQVLTEPHGGSDIANCQTKAIRDGDD
                     YIVNGQKVMVGHHLPPDYLWTLVCTNPQGKRHENLAWLHIPANLSGITIQHMYLMMGI
                     KNAVFFDNVRVPAKYLIGGENNGWKVASTHMELEHGGDGRIAADPLVERVVEYCRNTH
                     VGGKPLLEDPRVRDLVAEMMIDLNTLRRFNQLIYWRRFAKKPHPYGGPQQRYFQRMMR
                     LRNGERLQAIMGADALVQDLSVHEVIDFEYAVRTGPGQLHGGGTLDTDRLIFARRLGL
                     GRPTTEKAPETD"
     gene            2003..>2730
                     /locus_tag="VFY96_03880"
     CDS             2003..>2730
                     /locus_tag="VFY96_03880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5605628.1"
                     /translation="MDLSLTEDQEMLKKVAADFVKAEVPAHQMTQWYKNKQTFQPQLL
                     KKAAELGWLGMMLPEQYGGSELGATDCAVVFEELGRGPVPGPLFTSGIFAAQMILESA
                     TEEQKKSLLPQLCKAESIIVPAITDKAAYWGPEAVEARLAKTPGGYLMTGAKRFVFDA
                     QAATHFLCAARSEEDKVVFLIVDAKTPGVTITPHVGFLVSVAEVRFDNVRIAAENLLG
                     TSGACWATLESALDKALPILCAYQV"
BASE COUNT          622 a          759 c          788 g          561 t
ORIGIN      
        1 ggcgattgcg ttcatcgagt cacacgtcgt tgactatctg aacggcggcg aagcggtgtc
       61 gcgcgaaaat tttcctaagg gcaggatcta cagtttgctc gattgtgatg gaaagccctt
      121 catggttcat ttgtccggtc acaacgaggc gtgggaaggg ttgttgaaag cgggcgagct
      181 ccaggactta atcggcgact cgcgctttaa catgcgcaaa gcccgcaccg atcggcacga
      241 tgagatcgtc acgattctgc gtgagcagtt tcggcgcaaa ccacgcgatt actggctgcc
      301 gcggcttgac gccaatcaca ttcccaatgc gccgatcaat acgatcgctg aagttttcga
      361 aaatccgcag atcaagcaca tgggaattcc caagcagatc gctcatccca agctcggtac
      421 gagcaatcta gtgggcagcc cgatcaatct ctccgggaca cctgcgagat tttaccgagc
      481 ggcgccatta ttgggcgagc ataacgaaga agtcttggga aaattgggat acgacgatca
      541 atcactgaag gagttaagag caagtggaat aatatgacgc tcgtctagag tctcgcgtct
      601 aatgtctgat gttaacgctg gacgctggac cggcgacgct agactgaaga acaaaggagt
      661 aagccttatg gattttaacg tatctgccga tccgccccac gttgtcgagt ttcgtaacga
      721 ggtcgccgcg tggctcgccg agcagttcaa aggttatggc cacctgcgct ggtcggcttc
      781 ctggtcgacg cgcgaggatg acgaggaata taagtttcgc cgcggtcttg ccgaaaagct
      841 cggcgctaaa ggctggttgt ttccgattta tccaaagaag tacggcggcg ccgagctgac
      901 ggtcgatcac cagatcgtgc tcgaaaccga gctgtttaaa tacggactga atctttcgca
      961 tgtcttctac accctcgcgc gcatcgtcgc gccggtgctg atccgctggg gaacggaaga
     1021 gcaaaaagcg gaatttctcc cgcccatcac tcgggcccaa atttgtgttt ggcaagtcct
     1081 gacggagccg cacggcggca gcgacattgc aaactgccag accaaagcga tccgcgacgg
     1141 cgatgactac atcgtcaacg gccaaaaggt tatggtgggt catcacctgc cgcccgacta
     1201 tctctggacc ctggtttgta ctaatcccca gggcaagcgc cacgaaaacc tcgcatggct
     1261 gcacattccg gcaaatcttt ccgggatcac gattcagcac atgtacttga tgatgggcat
     1321 caagaacgcg gtgttcttcg acaacgtgcg cgtcccggca aaatacttga tcggaggcga
     1381 aaacaacggc tggaaagtcg ccagcacgca catggagctg gaacacggcg gcgacggcag
     1441 aattgccgca gatcctctgg tcgaacgggt ggtcgaatac tgtcgcaata cgcacgtcgg
     1501 cggcaagcct ctgttggaag atccacgcgt gcgcgatctc gtcgcagaga tgatgatcga
     1561 cctcaatacg ctccggcgct tcaatcagct catctattgg cgtcggtttg ccaagaagcc
     1621 gcacccctat ggcggccccc agcagcgcta cttccagcgc atgatgcgac tccgtaacgg
     1681 cgaacgcttg caagcgatca tgggcgccga tgcgctcgtg caagatttat cggtgcacga
     1741 ggtgattgat ttcgaatatg ccgtgcgcac cggcccggga cagcttcatg gcggtggcac
     1801 attggatacg gatcgactga tctttgcgcg gcggttgggc ctcggccggc caaccacgga
     1861 gaaagcgccg gagacagatt aatgaaggta cagccatgag gcaatctttc gatatgcgcg
     1921 ccccctcgat acgcgctccg cgctgctcgg ggtggacggg aatgtaaggg ccgcagcatg
     1981 ctgcgctctg taggagacga agatggattt atcactgacc gaagaccaag aaatgttgaa
     2041 aaaagtcgcc gccgatttcg tcaaggccga agtgccggcg caccagatga cccaatggta
     2101 caagaacaaa caaacttttc agccgcagtt gcttaagaaa gccgccgagc ttggctggct
     2161 cggcatgatg ttgccggaac agtacggcgg gtccgagctc ggcgccacgg actgcgccgt
     2221 ggtgttcgag gaattgggcc gcggcccggt acccgggccg ctctttacct ccggcatttt
     2281 cgccgcgcaa atgatcctgg agagtgccac cgaagagcaa aaaaaatcct tattgccgca
     2341 actttgcaaa gccgaatcga tcatcgtccc cgcaatcacc gataaggcgg cttattgggg
     2401 tcccgaagcg gtcgaagccc ggctggcgaa aactccgggc ggttatttga tgacgggagc
     2461 caagcgcttt gtcttcgatg cccaggcagc gacccacttt ctctgcgccg cgcgctcgga
     2521 agaggacaaa gttgtcttct taatcgttga cgcgaaaacc cccggcgtca cgatcacgcc
     2581 gcacgtcggc tttctcgttt ccgttgctga agttcgcttc gacaatgttc gcattgcggc
     2641 tgaaaatctg ctcggcacga gcggcgcatg ttgggcgact ctggaaagcg cactcgacaa
     2701 agcgttgccg attctttgcg cttaccaggt
//
LOCUS       DASULZ010000191         6303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1240505, whole genome shotgun sequence.
ACCESSION   DASULZ010000191 DASULZ010000000
VERSION     DASULZ010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240505"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VFY96_03885"
     CDS             complement(<1..580)
                     /locus_tag="VFY96_03885"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HEX5605629.1"
                     /translation="MSSAQRRSEILGTGSELPSKVVTNHDLEKMVETSDEWITVRTGI
                     KERRVLEEGKGNADMAFRAAQRALKDAQMQATDLDAIIMGTVTPDYPFPSSACVLEDM
                     LGARNVFSFDVGAACSGFLNALSVADSFIRTGQINNALVVGSDALSRLLNWQDRGTCI
                     LFGDGAGAVVLGASENGSGILSTRLRTDGSYVK"
     gene            complement(577..1821)
                     /gene="fabF"
                     /locus_tag="VFY96_03890"
     CDS             complement(577..1821)
                     /gene="fabF"
                     /locus_tag="VFY96_03890"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX5605630.1"
                     /translation="MQEQRRVVVTGLGVVSPLGTGVEKNWQALMAGRSGIRPITRFAT
                     EGFGARIAGEVPDFRAEDFIEPKEIKKMDLFIQYAICAAAMAMQDSGLKIEGEFAENV
                     GVIIGVGLCGLDTIETTHKALLEGGPRKISPFFIPKVISNLAAGHIAIRHGAKGVNWT
                     PTSACASGTHAIGEAFHLIRRGTQDAVVAGGAEAVITPLAVGGFASMKALSTRNEEPE
                     RASRPFDKARDGFVIGEGSGVLIIEEREHALRRGAKIYAEVVGYAANGDAYHMTSPAP
                     EGEGAARCMRLALKDAALTPHDVGYINAHGTSTEYNDANETTAIKNVFGEHAAKIAVS
                     STKSMTGHLLGAAGAVEGIYTVLALQQGILPPTINYETPDPACDLDYVPNTARKAEID
                     IALSNSFGFGGTNACVIFRRAQ"
     gene            complement(1901..2935)
                     /locus_tag="VFY96_03895"
     CDS             complement(1901..2935)
                     /locus_tag="VFY96_03895"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX5605631.1"
                     /translation="MGKPAVVIDPAYLKHLPGDSHPERPERIQVLLDLANALDRQKFN
                     LLAPRAATRAEIEGIHEPEHVRLVESTSSVNHYALDGDTITSRDSFAVSVLAAGGFLR
                     LLDSMAAKESSSGFALVRPPGHHASFDRAMGFCLFNTIAIGAAYLKKQYGAKKILIMD
                     WDVHHGNGTQNCFYDDPSVLFISTHQYPFYPGTGAINEVGAGPGEGYTINIPLPAGCS
                     DQEYMQVFQHLVVPAAENFQPEWILVSAGFDPHRRDPLGGMRVTEEGFGGMAGALSSV
                     AKRFSEARIALTLEGGYDLAGLRNSAAAVLSALQQGKAQQNRVLRLSESRIEPLIRRV
                     LKVHENYQQT"
     gene            3136..3408
                     /gene="tatA"
                     /locus_tag="VFY96_03900"
     CDS             3136..3408
                     /gene="tatA"
                     /locus_tag="VFY96_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX5605632.1"
                     /translation="MFGIGMPELLLILGLALIVLGPKKLPELARALGKGLAEFRRATD
                     ELKDEFRNMEREIDDASAVGNLKDDQVVESSAESPPAKPSVTPEKT"
     gene            3447..4178
                     /gene="tatC"
                     /locus_tag="VFY96_03905"
     CDS             3447..4178
                     /gene="tatC"
                     /locus_tag="VFY96_03905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX5605633.1"
                     /translation="MPFTSHLAELRSRLIKSTLAIAVAFSVCYAFVDDIFALLAAPVR
                     QLRIEGLTLIGTTVTEAFFTRMKVSFVAAIIVAAPVWLWQAWQFVAPGLYEHEKRYTR
                     SFVFFGSLFFAAGAAFCYLVVIHFGLGFLLHRYEAIHIQPLLQVGDYLSLVSRLVLAF
                     GVMFELPVLAFFLARVGIIDHRFLINHSRYGVIAIALLAAVLTPPDLISQVLLMAPLM
                     LLYAASIAAAYAARYRMNRLERDAG"
     gene            complement(4250..4801)
                     /locus_tag="VFY96_03910"
     CDS             complement(4250..4801)
                     /locus_tag="VFY96_03910"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HEX5605634.1"
                     /translation="MIPKKCFFTKGVGVHKEKLASFELALRAAGLAYCNLVLVSSIYP
                     PGCKRISKEEGVKLLRPGEIVFCVYDRESTNEPNRLVAASVGVAIPADQEQHGYLSEH
                     HSFGETEERAGDYAEDLAASMLATTLGIEFDPDTAWDERENLFKMSGKIVRTSNITQS
                     AIGNKDGLWTTVFAACAFVNEDS"
     gene            complement(4873..5376)
                     /locus_tag="VFY96_03915"
     CDS             complement(4873..5376)
                     /locus_tag="VFY96_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX5605635.1"
                     /translation="MGFVAKLGEQSYSVEIEEIGKSLYRVAVDGNEFLVDGKKTGRTN
                     YSLIVDNRSFEIEVDHTEDQYRVLVDGRNYHVHLVDERKIRVGGTQSGLELQGRQLVS
                     VPMPGKIIAVLVAEGDPVEKGQGLVIVEAMKMENEVRSPVTGAVREIKVKPGDTVEGG
                     AVLLVVE"
     gene            complement(5380..>6303)
                     /locus_tag="VFY96_03920"
     CDS             complement(5380..>6303)
                     /locus_tag="VFY96_03920"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="catalyzes the ATP-dependent carboxylation of a
                     covalently attached biotin and the transfer of the
                     carboxyl group to pyruvate forming oxaloacetate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX5605636.1"
                     /translation="IEIQVLADNYGHVIHLYDRECSIQRRHQKVIEESPAPGMDDRTR
                     RRIGRIAIQGARAVGYVGAGTLEFLLDPEQNFYFLEMNTRLQVEHAVTERVVGIDLVK
                     AQIEVAAGGYLPWRQRHITQTGHAIECRIYAEDPERDFMPCPGKIEGLRLPEGLGLRN
                     DCGVYEGAEVPIYYDPMIAKLITWGENRVEAILRMRRALREYQVRGIKTNIAFNQWIV
                     RHTRFMSGDFNTGFIDEEYRVTDGEEILPHKEIALASAAIAALHREQEQNLGLLAQGA
                     AEPSKWRDQAKRNALRRHAAFVDRGWYRPRG"
BASE COUNT         1332 a         1838 c         1678 g         1455 t
ORIGIN      
        1 gcttgacgta ggagccgtcg gttcgtaacc gggtgctcaa gatccccgag ccattctccg
       61 aagcgcccaa gactacagcg ccagcgccgt caccgaacag aatgcaggta ccgcgatctt
      121 gccaattgag caggcgactg agggcgtcgg agccgaccac aagggcgttg ttgatctgtc
      181 cggttcgaat gaacgaatcg gccaccgaca gcgcgttcaa aaatcccgag catgctgccc
      241 ccacatcaaa agaaaaaacg ttacgtgcgc caagcatatc ttcgagcaca cacgccgagc
      301 tcggaaacgg ataatcggga gtgacggtgc ccatgataat ggcatcgagg tcagtggctt
      361 gcatctgcgc gtccttgagc gcgcgttgag ccgcgcggaa tgccatgtcg gcattccctt
      421 ttccctcttc gagtacccgc cgttctttga ttccggttcg aaccgtaatc cattcgtcgc
      481 tggtttcgac cattttctcc agatcgtgat tggtcacgac cttgctcggc aattctgatc
      541 cggtgcccag tatctcgctt cgccgttgag cgctgctcac tgagccctcc gaaagatcac
      601 gcaggcattg gttcccccga acccgaacga atttgacagc gcgatatcga tctcagcttt
      661 gcgtgcggtg ttaggaacat aatcgagatc acaggcgggg tcgggagttt cgtagttgat
      721 ggtcggcggc agaatgcctt gctggagcgc aagaacggtg tagatacctt cgacggcacc
      781 ggcggcgccc agcaaatgac cggtcatcga cttggtcgag ctcactgcga tttttgcggc
      841 atgctcgccg aatacgtttt tgatcgcggt ggtttcgtta gcgtcgttgt attcggtcga
      901 agttccgtgc gcgttgatat agcccacgtc gtgcggcgtc agtgcggcat ctttgagcgc
      961 cagccgcatg caacgcgctg cgccttcacc ttcgggggcg ggcgacgtca tatggtaagc
     1021 gtcgccattg gcggcatatc cgacgacttc agcgtagatt ttcgcgccgc gtcgaagcgc
     1081 gtgctcgcgc tcttcgatga tcaaaactcc cgagccctca ccaatgacga agccatcgcg
     1141 agctttatca aacggtcgac tggcgcgttc gggctcctca ttgcgcgtcg agagcgcttt
     1201 catcgaagcg aaacccccga ccgccagggg cgtaattacg gcttctgcgc cgccggcaac
     1261 gacagcatcc tgtgttcccc gacggatcaa gtggaaagct tcgcctatgg cgtgtgttcc
     1321 tgaagcgcaa gccgacgtcg gggtccaatt tacccctttt gcgccgtgtc ggatcgcgat
     1381 gtggcccgcc gccagattgg aaataacttt cggaatgaaa aatggcgaga tctttcgcgg
     1441 cccgccttcg agcagagcct tgtgcgtggt ttcgatggtg tccaggccgc ataaaccgac
     1501 gccgatgatg actcccacgt tctcggcaaa ttcaccttcg attttcaaac cactgtcttg
     1561 catcgccatc gctgccgcgc agatggcgta ctggatgaag agatccattt tcttgatctc
     1621 tttgggctcg ataaaatctt ccgctcggaa gtccggcacc tctccggcaa ttcttgcgcc
     1681 aaatccctcc gtggcaaaac gcgtgatcgg acgaatgccc gagcggcccg ccatcaaagc
     1741 ttgccagttt ttttcgacgc ctgttcccag tggggaaacc acgccgagcc ccgtcacaac
     1801 aactctgcgt tgctcctgca ttttctagct tgtaaccgat ttgtaacgat taaagaggta
     1861 agatagatga gtcggcgcag acggtcaata gcagtagcga ttaggtttgt tgataatttt
     1921 catgaacttt caaaacgcgg cgaatgagcg gctcgatgcg gctctcggat aaacgcaaaa
     1981 ctctgttttg ctgcgctttt ccttgttgta gcgcactcag gaccgctgcc gcggaattgc
     2041 gcaatccggc caaatcgtaa ccgccttcga gcgtcaaagc gattcgcgcc tcggaaaagc
     2101 gctttgccac cgaagacaac gcgccggcca tgccgccgaa accttcttca gtgaccctca
     2161 taccacccag cggatcacgc cgatgcggat caaagccggc agaaaccaga atccactcgg
     2221 gctgaaagtt ttccgctgcg gggacgacga gatgctgaaa gacttgcata tattcttgat
     2281 cggagcagcc cgcgggcagc ggaatgttaa tcgtatatcc ttctcctggt ccggcaccga
     2341 cttcattgat cgctccggtg cccggataaa acggatattg atgggttgaa atgaacaaca
     2401 ccgaggggtc gtcgtaaaag caattttgcg tcccgttgcc gtgatgtacg tcccagtcca
     2461 tgatgagaat cttctttgcg ccgtactgct tcttgagata cgccgcgccg atcgcgatcg
     2521 tgttgaacaa gcaaaatccc atcgcgcgat caaacgaggc atggtggccg ggcgggcgca
     2581 ccaatgcaaa accgctggag ctttctttcg ccgccatgct atcgagcagc ctcagaaaac
     2641 cgcccgccgc caggacgctc accgcaaaag aatcgcggga agtaatcgtg tctccatcga
     2701 gggcgtagtg attgactgaa gaagtcgact ccaccagtcg aacatgctcc ggttcatgga
     2761 tgccttcaat ctcggcacga gtcgccgctc gcggtgcgag cagattgaat ttctgccgat
     2821 cgagcgcatt ggccaaatcg agcagaacct ggatccgctc aggtctttca ggatgcgaat
     2881 cgcccggcaa atgtttcaaa taggcgggat cgataaccac cgcaggcttt cccatggcaa
     2941 caagctaaca cctgaaatct ggcgctgcaa agaaccgcgc tgccgggatc catagggtat
     3001 ttctgtgtcg aggccaatta ccgaatcacc cgacaatcgt caccacgccg gacgcggtgc
     3061 gcagcgatcg cgcgcgaacg cttgagattt gccgccaaac ttgccaagcc gcaacaaatc
     3121 gaataacttg attgcatgtt tggcattggg atgccggaac tgctcctgat tcttggtctg
     3181 gcacttatcg tgctcggtcc gaaaaagttg ccggagctgg cgcgcgcact aggcaaaggc
     3241 ttagcggaat ttcgccgggc cacggacgag ctgaaagacg aatttcgcaa catggaacgg
     3301 gaaatcgatg acgcttcggc agttggcaat ttgaaggacg atcaggtcgt ggagagctcg
     3361 gcagaatctc ctccagccaa accatcagtc actcccgaaa agacgtgagc aacagcgaag
     3421 ggaatggaca ttccagcgat gtccaaatgc cctttacctc acatctcgcc gagctccgct
     3481 ctcggttgat caagagcacc ctcgcaatcg cagtcgcttt ttccgtttgt tacgcctttg
     3541 tcgacgatat ttttgcgctg ctcgccgcac ccgtaagaca actccgcata gaggggctaa
     3601 ccctgatcgg cacgacggtc acggaagctt ttttcaccag gatgaaagtt tctttcgtcg
     3661 cggcgattat cgttgcggcg ccggtctggc tttggcaagc atggcagttc gtcgcgccgg
     3721 gtctctacga acatgagaag cgttacactc gcagtttcgt cttcttcggt tcacttttct
     3781 ttgccgccgg cgcggccttt tgctatttgg tcgtgattca tttcggcctt ggatttctat
     3841 tgcaccgcta cgaagcgatt catatacagc ctctattgca ggtcggtgat tacttatctc
     3901 tggtttctcg tctcgtgctt gccttcggcg tcatgtttga actccccgtg cttgcttttt
     3961 ttcttgcgcg ggtcggcatt atcgaccatc gttttctgat caatcactcc cgctacggtg
     4021 tgatcgccat tgctttgctc gccgccgttt tgacgccgcc ggatttgatc tcgcaggtgc
     4081 ttctgatggc accgctgatg ctgctctacg ccgcgagcat cgccgcagct tatgccgcac
     4141 gctatcgcat gaaccggctg gagcgggacg ccggatgaat gtggagtgtt ggagtgatgg
     4201 gtataggagg cgagcgtcca gcgctcgatt actccaagtg agtttgcgct tagctgtctt
     4261 cgttgacgaa agcgcaggcg gcaaagaccg tcgtccaaag cccgtctttg ttgccgatgg
     4321 cggactgcgt aatattcgaa gtacggacaa ttttcccgga cattttgaac agattctctc
     4381 tttcgtccca agccgtatcc ggatcgaatt caatgccgag cgttgtcgcc aacatgctcg
     4441 cagctaaatc ctcggcgtaa tcccctgcgc gctcttcggt ttcgccaaag gagtggtgct
     4501 ctgaaagata gccgtgctgc tcctggtcgg ccggtatggc aacccccacc gaagcggcca
     4561 ccaaccgatt tggctcgttc gtgctttctc tatcataaac acaaaagacg atttcacccg
     4621 gccgtaggag cttaacacct tcttccttgg aaatccgttt gcagcccggc ggatagatac
     4681 tggagaccaa cacgagattg caataggcca atccagctgc gcgtaaagcc aactcaaagg
     4741 aagccaattt ttccttgtga acaccgactc ctttggtgaa aaaacatttt ttagggatca
     4801 tgctgggctg ctataacatg attggaattg ggtgacaaca ttttcgcgca ggaaatgaat
     4861 caaaaaataa ttttactcga caacgaggag caccgcacca ccttccaccg tatcgcccgg
     4921 tttcactttg atctctcgaa ctgcgccggt cacgggactg cgcacttcgt tttccatttt
     4981 catggcctct acgatcacca atccctgtcc cttttcgacc gggtccccct cggctaccaa
     5041 aacagcgatg attttaccgg gcatcggcac ggaaaccagt tgccgacctt gcaactccag
     5101 gcctgactga gtcccgccca cgcgtatttt gcgctcatca accagatgga catgataatt
     5161 ccgtccgtcc acgagcaccc ggtattgatc ctcggtatga tccacttcga tttcgaacga
     5221 acgattatca acgatgaggg agtagttcgt ccggccggtt tttttcccgt cgacgagaaa
     5281 ttcgttgccg tccaccgcca cgcgataaag cgatttgccg atctcttcga tctcgacgct
     5341 gtagctctgc tcgccgagct tggcgacaaa tcccacggat caacctcgag gacggtacca
     5401 accgcgatcc acgaacgcgg cgtgacggcg caaggcgttg cgctttgcct ggtcgcgcca
     5461 tttggacggt tcggcggcgc cctgcgcgag caatcccagg ttctgctcct gttcgcggtg
     5521 gagcgcggca atcgccgcag aagccagagc aatctcctta tggggcaaga tttcttcgcc
     5581 gtcagtcact cggtactctt cgtcgataaa tcccgtattg aaatcaccgg acataaaacg
     5641 ggtatgacgc acgatccatt ggttaaaagc gatattggtc ttgatgccgc gaacctgata
     5701 ttcccgcaac gcgcggcgca tgcggagaat tgcttcgact cggttttcgc cccaggtgat
     5761 caacttcgcg atcatcggat cgtaataaat cggcacttcc gcgccctcat agacaccgca
     5821 gtcgttgcgc agccctagcc cctcgggcag gcgcaaccct tcgattttcc cgggacacgg
     5881 catgaaatca cgctccggat cttccgcgta gatgcggcat tcgatggcat gaccggtttg
     5941 cgtaatatgg cgctggcgcc atggcagata gccacccgcc gcgacttcga tttgcgcttt
     6001 gaccaggtcg atgcccacga cgcgctcggt cacggcatgc tccacttgta gccgcgtgtt
     6061 catctcgaga aaataaaaat tctgctccgg gtcgagcaga aactcgaggg ttcccgcgcc
     6121 gacgtaaccc acggcgcgag cgccttgaat cgcgattcgg ccgatgcgcc ggcgcgtgcg
     6181 atcgtccatg ccaggggcgg gcgattcctc gatcactttt tgatggcggc gctggatcga
     6241 acactcgcga tcatacagat ggatcacatg accgtaatta tccgccagca cttgaatctc
     6301 gat
//
LOCUS       DASULZ010000192         4521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_936541, whole genome shotgun sequence.
ACCESSION   DASULZ010000192 DASULZ010000000
VERSION     DASULZ010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936541"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VFY96_03925"
     CDS             complement(<1..744)
                     /locus_tag="VFY96_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011138740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase cytochrome b subunit"
                     /protein_id="HEX5605637.1"
                     /translation="MNNQAELDVIAGVGLDQRTRKSHWPARLDFVQSASGLFLGLFMW
                     GHMAFVSTILISNDAMWWVTRMFEGQFFFGKRYPILVGAIVALVATMFVLHGLLAMRK
                     FPGNFQEYRSFAGHRKMLRHEDTTLWWFQAVTGFALFFLASVHLYQMLAHPGDIGPYE
                     SADRVWSGNWWPLYLVLLFNVELHGGIGLYRLAVKWGWFEGEDPNRTRQVLKRVKWGI
                     TVFFLVLGLTTLAAYMKIGHEHRDRAGERY"
     gene            complement(997..2412)
                     /locus_tag="VFY96_03930"
     CDS             complement(997..2412)
                     /locus_tag="VFY96_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEX5605638.1"
                     /translation="MNVSRADRRKLSKQALPFVVAIGIWFTPIPPGLTSEAWHLFAVF
                     AAAIFSVITNAFPLLTASLLAVGAVVLTDSVDATKAFAGFANSSVLLVVVAFLVAHAV
                     VKCGLGRRISLLVVSAFGRSTLGLGYSIFITDALIAPAFPSNTSRGGVLYPIILSLAE
                     SAGSRPEDAATRRLGGYLMFCGMASLSVSSALWLTATSANPIGVSLAAQSGVRIAFGT
                     WLLAASVPALATILMLPVVLARLFPPGVTETPHGPAMARKELHSMGPLSRDEWIVAIT
                     FLIMVAGWVAADSFRLSLPAVAFGGLGVLLATNVLTVEDINLQGSTLVTFFWLAVLFA
                     LSGQLNELGFMGYVGAKLTALLSGVAWPVAYVVVLVLYVLMHYMFVSQSAQVLALFGV
                     FLDVAVRAGVPRSLMAFALLFASSYFSTITPQGGSQNVIFVGSNYLTQVELYRLGLFT
                     TGFCLLSFLVLGTPWLLLVAR"
     gene            complement(2409..2927)
                     /locus_tag="VFY96_03935"
     CDS             complement(2409..2927)
                     /locus_tag="VFY96_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605639.1"
                     /translation="MALLLIAACGQPEHPTPQTSSPGEWQVFEGTWTAAGTRRMLRLG
                     RDDRAAIFELTGSVLLTGAQRPAVGFRAQAIGFSDSRAGMQGRCVWTDERGDMVYSEL
                     KGEAVGSGNHIVGTFIGGTGRYQGVTGDYSFQWQYVIDAEDGAVSGRVVNFKGRARLS
                     EASSAPPGRPAA"
     gene            complement(3039..3623)
                     /locus_tag="VFY96_03940"
     CDS             complement(3039..3623)
                     /locus_tag="VFY96_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605640.1"
                     /translation="METERPVRSYGFRIVVLLAVLANLILVIALITQVRELQQRVASL
                     PPDLASKRDVAMLRPLRVREILMQNCVECHSSRRLGTTISMEPRDLQRTLQRMQTHPG
                     ANIAPGDFDRIAASLLVARCARCHGEETLNLMVLKTHPERIATIRRMAALPGSGVRSD
                     QVLAIAQAFDKLIDNSQSGGTDSNDTLPAVQPRK"
     gene            complement(3625..>4521)
                     /gene="nrfD"
                     /locus_tag="VFY96_03945"
     CDS             complement(3625..>4521)
                     /gene="nrfD"
                     /locus_tag="VFY96_03945"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX5605641.1"
                     /translation="AITMLMVELGRPWMVWMVLVSWAPTSALYEVGWCAFLYLSVLAL
                     EFAQVPLEQAGWHRAFRIIRAIYIPIMLLGVTLSHLHQSSLGTLMTLIPHKINALWWS
                     DNLPLLYLFSAMMAGPAMAILEHIAAARWLGFAPRMELLGGLARIECWLVGLFLAFQM
                     GDLFYRGAADAMFIASWFAVSFWVEIVFGLLVPFVLLMMPEVRESRRGLATACALIIA
                     GVLLHRLNVAVIGLRVRHWETYMPSIGEIAITFGITAAAIFVFGWLARILPIHEELPA
                     PETRPRPATQPAGVLQRVEGVS"
BASE COUNT         1058 a         1382 c         1306 g          775 t
ORIGIN      
        1 gtagcgctcg ccggcgcgat cgcgatgctc gtggccgatc ttcatgtaag ccgctagcgt
       61 cgtgagtccg aggaccaaga agaaaaccgt gattccccat ttgactcttt taagcacttg
      121 acgcgtccgg ttgggatctt cgccttcgaa ccaaccccat ttcaccgcca accggtagag
      181 gccgatgccg ccatgcagtt ccacgttgaa cagcaaaacc agataaagcg gccaccagtt
      241 gccgctccac actcgatccg ctgactcata cgggccgata tccccggggt gtgcgagcat
      301 ctggtaaaga tggacggaag cgagaaagaa aagcgcgaaa ccggtcaccg cctgaaacca
      361 ccaaagagtt gtgtcctcgt ggcgcagcat ttttcgatgg cccgcgaaac ttctatactc
      421 ctggaagttg cccgggaatt tgcgcatggc gagcagtccg tggagaacga acatggtagc
      481 caccaaggcg acaatcgctc cgacgagaat cggatatctc ttgccgaaga aaaattgtcc
      541 ctcaaacatg cgcgtcaccc accacatggc atcgttgctg atcagaatcg tcgacacgaa
      601 agccatgtgt ccccacatga agagaccgag aaacaatccg ctcgcgctct gcacaaagtc
      661 cagccgtgcg ggccagtgac tttttcgcgt tctttgatcc aatccgacgc cggcaatgac
      721 atcgagctcg gcttggttat tcattggcaa tgctcctatc ccgcgcgcgt tgacaaatcg
      781 gcgagctgat tctcaagcaa cttctccaag ggttcaccta ccatgtctag cgacaattta
      841 agatgcggta tattggactt tagtccaaca ggggcacgtc cgatcgtgag gcgtcggatg
      901 gtaatctctt ggcagtaaag caatggcaaa ccgcatcgcc atcactactg atgtactgcg
      961 cttcgtgatc tctatcgtca actttgtgtg cccttgtcat cttgcgacca aaagcaacca
     1021 gggcgttccc agcaccagaa acgagagtag gcaaaagccc gtggtgaaaa ggcctaagcg
     1081 atagagctcg acctgggtca aatagttgct gccaacgaag ataacgtttt ggctgccgcc
     1141 ctgcggagta atcgtcgaga agtagctgct ggcgaatagc agcgcgaatg ccatcaaaga
     1201 tctcgggacg ccggcgcgca cggcgacatc gaggaataca ccgaacaatg cgaggacctg
     1261 cgccgactgg ctcacgaaca tatagtgcat gagcacgtag agcacgagca caacaacgta
     1321 ggccaccggc caggctacac cgctgagcag cgcggtaagc ttggcgccga catagcccat
     1381 gaaaccgagc tcattcaatt ggccactcaa cgcgaaaagc accgcgagcc aaaaaaaggt
     1441 gactagggta ctgccctgca ggttgatgtc ctcgacggtc agaacattgg tcgcgaggag
     1501 cacgcccagg ccaccgaagg ccaccgcagg aagactcaga cggaagctgt ccgccgccac
     1561 ccatccggcc accatgatca aaaaagttat tgcgacgatc cactcgtcgc gactgagcgg
     1621 ccccatgctg tgcagctctt tgcgcgccat cgcgggccca tggggcgtct cggtcacccc
     1681 tggcgggaag agtcgggcaa ggaccacggg cagcataagg atggtcgcca atgccggaac
     1741 cgacgcggcc agaagccatg tgccgaaggc gatcctcaca ccggattgcg cggcaagcga
     1801 cacgccgatc gggttggcgg aggtggcggt aagccatagt gccgacgaga cgctcaggct
     1861 cgccatgccg cagaacatga gataaccacc caggcggcgc gtggccgcat cttcaggcct
     1921 ggaaccagcg ctctccgcta gcgatagaat gatcgggtag agcacgccgc cacgcgacgt
     1981 attgctcggg aaggccggcg cgatcagcgc gtcggtaata aaaatgctat agcccagtcc
     2041 gagcgtcgat cgtccgaagg cgctcaccac gagcaggcta atgcgacgtc caagtccgca
     2101 ctttaccacc gcatgcgcaa ctagaaacgc aaccaccacc agaagtacgc ttgagttggc
     2161 aaagccagcg aaggctttgg tcgcgtctac actgtcggtc agcacgaccg cgcccaccgc
     2221 gagcagcgag gctgtcagca gcggaaacgc gttcgtgatg accgagaaaa tcgcggctgc
     2281 aaaaaccgca aaaaggtgcc acgcctcgct cgtcagcccg ggcggaatcg gcgtgaacca
     2341 gatgccgatt gcaacgacga atgggagggc ttgtttgctg agcttgcggc gatcggcacg
     2401 ggacacattc atgcggccgg ccttcctgga ggcgcactag atgcttcact taaccgcgcc
     2461 cggcccttga agttcacgac gcggccgctc accgcaccgt cttctgcatc gatcacgtac
     2521 tgccactgga agctatagtc gccggtcacg ccttggtaac ggcctgtccc gccaatgaat
     2581 gtgcctacga tatggttgcc gctgccgacc gcctcgccct tcaactcgct gtaaaccatg
     2641 tcgccgcgct cgtcggtcca gacgcatcgg ccctgcatgc ccgcgcggct atcggagaag
     2701 ccgatcgctt gcgccctgaa gccgaccgcc ggccgctgcg cgccggtgag cagcacagag
     2761 cctgtcagtt cgaagatcgc cgcgcgatca tcgcgaccca aacgaagcat tcggcgcgtg
     2821 cctgctgcag tccaggttcc ctcgaagacc tgccattcgc cagggctcga agtctgtggc
     2881 gtgggatgct ccggctggcc gcacgcagca atgagcagca gagccacgaa catccgccat
     2941 ctcaactcgg gcagcaacaa gaactgtggc tctccgcgtt tccgcgcaca acatttaccc
     3001 tcaattagcg tcaatcgttg ggattaggca gctttggcct atttgcgcgg ctgaactgcc
     3061 ggcaaggtat cgttgctatc agtgccgccg ctctgactat tatcgatgag cttgtcgaac
     3121 gcttgcgcga tcgcgagtac ctggtcggag cgcacgcccg aaccggggag cgccgccatg
     3181 cggcgaatcg tcgcgatgcg ctcaggatgt gtctttagca ccatcaggtt aagggtctcc
     3241 tcgccatgac agcgcgcgca gcgggccacc agaagagacg cggcaatgcg gtcaaaatcc
     3301 ccgggcgcga tattggcccc gggatgcgtt tgcatacgct ggagcgtgcg ctgaagatct
     3361 cgtggttcca tcgaaatcgt cgtgccgaga cggcggctgc tatggcactc cacacaattc
     3421 tgcatcagga tctcacgaac ccgcagcggc cgtagcatgg cgacatcgcg tttgctggcc
     3481 agatcgggag gcaggctcgc gacccgctgc tgcagctcac gcacctgagt gatgagggcg
     3541 atcacaagga tcagattggc gagaaccgcc agcaggacca cgatgcgaaa cccgtagctt
     3601 cggaccggtc gttccgtttc catatcacga gactccttcg acacgttgca acacgcccgc
     3661 cggctgcgtc gcggggcgtg gcctcgtctc tggggccggc aactcttcat gaatcggtaa
     3721 aatgcgtgcg agccaaccga acacaaaaat cgccgccgca gtgatgccga aggtaatggc
     3781 gatctcgccg atcgaaggca tataggtttc ccagtgccgc acacgcaggc caataacggc
     3841 gacgttcagc cggtggagta atacaccggc gataatcaag gcgcatgcgg tcgcgagtcc
     3901 gcgccggctc tcccgtactt ccggcatcat taggagaaca aacggaacca agagaccgaa
     3961 aacgatctcg acccaaaagg agacggcgaa ccaggaagca atgaacatgg cgtccgctgc
     4021 accgcggtaa aaaagatcac ccatctgaaa agcgagaaac aggccgacaa gccagcattc
     4081 gatgcgagcg agacccccga gcagctccat gcgcggtgcg aagccgagcc aacgagcggc
     4141 ggcgatgtgc tcgagaatcg ccatcgccgg gccggccatc atcgcggaga aaagatagag
     4201 caacggcaaa ttatccgacc accagagggc gttaatcttg tgcgggatca gcgtcatgag
     4261 ggttccgagc gacgattgat gcaggtgtga cagggtcacg ccaaggagca tgatcgggat
     4321 gtaaatcgcg cggatgatgc gaaacgcgcg atgccagcct gcctgctcca gcggcacctg
     4381 cgcaaactcc agagcgagca cagagagata gaggaacgcg caccagccta cttcgtacaa
     4441 cgcggatgtc ggtgcccacg acacgaggac catccaaacc atccatggcc ggccaagctc
     4501 gaccatgagc atggtaatgg c
//
LOCUS       DASULZ010000193         2911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_23959, whole genome shotgun sequence.
ACCESSION   DASULZ010000193 DASULZ010000000
VERSION     DASULZ010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23959"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="VFY96_03950"
     CDS             <1..1137
                     /locus_tag="VFY96_03950"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5605642.1"
                     /translation="GSFISGPYSAMLLADLGAEVIKVENPNGGDPFRGWDFGGDQPNF
                     WAYNRGKKSVTVNLQTPQGKEVFYGLVKKSDVVIDNYRPGVTKKLGIDFESLSKINPK
                     IVCCSITGTGPTGPYVKRPAYDTVGQGMGGMMSVLMDPHNPKPVGPAYADCLSGMFSA
                     LGILGALVARSQTGRGQQVDATMVGSILAFLNAPATETLAHGRVPGPYTRPRQSQTYA
                     FACADGAMLAIHLSSPQKFWEGLCKSVGHPELIDDPRFNTRPNRRTNYDDLQKTFAVF
                     FKEQPRSVWIQKLEANDVPHTPVYNLKEVFDDPQVQHMGLQIAIERKDKPTIRTVKFP
                     LNYSDTPSAHPTPPPELGENNTEFLSSLGYDKKKIAEFKDKGII"
     gene            complement(1326..2033)
                     /locus_tag="VFY96_03955"
     CDS             complement(1326..2033)
                     /locus_tag="VFY96_03955"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5605643.1"
                     /translation="MKLVNRDLNPETDGVPGYLAHPDCKEPGPGVLIVHHHYGVTGNM
                     KVNVCNFAKVGYTTLVLDLYKMLGESDGVHEAQKKTTDRQFVEILNSAWRFLTSRPEV
                     DAKRCAVVGYCMGGRIGIHFVAATPSVRAFVGYYPSVRDEEPSNLRPRHPNDAVKDFR
                     CPSQIFFGAKDHVASVPVQQKLMASFQANGQHLDWHFFHRAGHGFAMADGDCYDPKLA
                     EQAWSLVSEFLAAELGS"
     gene            complement(2058..>2911)
                     /locus_tag="VFY96_03960"
     CDS             complement(2058..>2911)
                     /locus_tag="VFY96_03960"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5605644.1"
                     /translation="TMTRGMIIIDSQVHIWAPETPDKPYARGDASPPHRPMPLGHEEL
                     LREMDGSGVARCVLVPPTWEADRNDTSLEAARLHPDRFRVMGRLALNKPESRELMPTW
                     KNQPYMLGIRVAFNAGNTRKWLEDGTADWFWDAAERYDIPVMAFAPNAVPKLEEIAER
                     HPGLRMIIDHMGLNSSLRGKSLEPAVANTLKFARLPNVAVKVSALPCYVDEPYPFPTL
                     HPLVRRVVDAFGPQRCFWGTDLSHLPCPYKQVVTLFTEEMKCLSGSELEWVMGRGIAE
                     WLGWPLP"
BASE COUNT          661 a          829 c          789 g          632 t
ORIGIN      
        1 ggtagtttta tttccggtcc gtacagcgcg atgttgctgg cggatttggg cgcggaagtg
       61 atcaaagtag aaaatcccaa cggcggcgat ccatttcgcg gctgggattt cggcggtgat
      121 cagccgaact tttgggctta caatcgaggc aaaaagagcg tgactgtgaa tttgcaaacg
      181 ccccaaggca aggaggtctt ttacgggcta gtcaaaaagt ccgacgtcgt aatcgacaat
      241 tatcgtcccg gggtgacaaa aaaactcggc atcgatttcg aatcgctaag caaaatcaat
      301 ccaaagatcg tttgctgctc gattaccggc acgggaccga ccggtcctta cgtcaaacgg
      361 ccggcctacg acactgtggg tcagggcatg ggcgggatga tgagcgtgct gatggatccg
      421 cacaatccca aaccggtggg accggcatac gcggattgtt tgagcggcat gttttccgcg
      481 cttgggattt tgggcgcttt ggtggcgcgc tcgcaaaccg gccggggcca gcaggtggat
      541 gcgacgatgg tggggtcgat tctcgctttc ctcaatgccc ccgctacgga aaccctcgcc
      601 catgggcgag tgccaggacc gtacactcga ccgcgccagt cgcaaacgta cgccttcgcc
      661 tgtgccgacg gcgccatgct cgcaattcac ttatcttcgc cgcagaaatt ttgggaagga
      721 ttgtgcaaat ccgtcggcca cccagagctg atcgatgacc cacgtttcaa cacgcggccg
      781 aatcgccgga cgaactatga cgatctccaa aagacattcg cggtattttt caaagaacag
      841 ccgcgttcag tttggatcca aaagctggaa gccaacgacg tgccgcacac accggtctac
      901 aatctgaaag aagtttttga cgatccgcaa gttcagcaca tgggtttgca gatcgccatc
      961 gagcgtaaag acaagccgac cattcgaacc gtcaaatttc cgttaaacta cagcgatacc
     1021 ccgtcggctc atcccacacc gccccccgag ttgggcgaaa acaacacaga atttttatct
     1081 tcgttaggat acgacaagaa aaaaatcgct gagttcaaag acaaaggaat tatctgacgc
     1141 ccagctcgcg cggattactc aggatctaag tcattccggg caagctggag atacgtagcg
     1201 cgatcggagg atttggtacg ggcgcatggc atgcgccctc tggatgccgg ttttcaccgg
     1261 catgacgaca atggaccctc gtagtttttc tatgaattga tattgcgcgg acaacagcat
     1321 agctttcagc ttccgagttc tgcggcgaga aactccgaga ccagactcca ggcttgttcg
     1381 gcgagtttcg gatcataaca gtcgccatcc gccatggcga atccatgccc cgcgcgatga
     1441 aagaagtgcc aatccaagtg ctgtccattc gcttgaaaac ttgccattaa cttttgctgt
     1501 accggaacgc tcgcaacatg atctttggcg ccgaagaaaa tctgcgacgg gcatctaaaa
     1561 tccttcaccg catcgttcgg atggcgcggc cttaggttac tcggttcttc gtcgcgcacc
     1621 gacggatagt agccgacgaa agcgcgcacg ctcggagtcg ccgccacaaa atgaatgccg
     1681 atgcgtccgc ccatacagta gccgaccact gcgcagcgtt tggcatccac ttccggtctc
     1741 gatgtcaaaa accgccacgc gctgttcaaa atttctacaa attgtctatc cgtggttttt
     1801 ttctgcgcct cgtggacgcc gtccgactcg ccgagcattt tgtacaggtc cagcactagc
     1861 gtcgtataac cgaccttggc gaagttacag acgttgacct tcatatttcc tgtgacgcca
     1921 taatgatgat gcacgatcag cacaccgggg cccggctcct tacagtcggg atgggcgaga
     1981 taacccggca cgccgtctgt ttctgggttc aaatctcgat tgacgagctt cattctccat
     2041 cctccagtta ctacggacta cggcaaaggc cagccgagcc attccgcgat cccgcggccc
     2101 atgacccatt cgagctcgga gcccgacaga catttcattt cttcggtaaa gagcgtcacg
     2161 acttgtttat acgggcaggg aagatgtgaa agatcggtgc cccagaagca gcgctgcgga
     2221 ccaaacgcat ccaccacccg ccgcaccagt ggatgtaagg tcggaaacgg atagggctcg
     2281 tcgacatagc atggcaacgc ggaaactttg acggcgacgt tcggcaagcg cgcgaactta
     2341 agtgtattgg cgactgccgg ctccagtgat ttgccgcgca ggctcgagtt cagtcccatg
     2401 tggtcgatga tcatgcgcag cccggggtgg cgctcggcga tttcttcaag cttcggtacg
     2461 gcattcggtg cgaacgccat gactggaata tcgtatcgct cggcggcatc ccaaaaccaa
     2521 tcggcggtgc cgtcctcgag ccattttcgc gtgttgccgg cattgaacgc gactctgatt
     2581 cccagcatgt aaggttgatt tttccaggtc ggcatcagct cgcggctttc cggcttgttt
     2641 agcgccagcc ggcccatcac ccgaaaacga tcggggtgca aacgagctgc ctcgagagaa
     2701 gtatcattac gatcggcttc ccacgtgggc ggcacgagca cgcagcgcgc cacgccgctg
     2761 ccgtccatct cgcgaagcag ctcttcatgt cctaacggca tcggccgatg cggcggtgat
     2821 gcatctcctc tcgcgtacgg tttatcggga gtttcgggag cccaaatgtg aacttgcgaa
     2881 tcgataatta tcataccgcg agtcatagtc c
//
LOCUS       DASULZ010000194         4255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1140035, whole genome shotgun sequence.
ACCESSION   DASULZ010000194 DASULZ010000000
VERSION     DASULZ010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140035"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VFY96_03965"
     CDS             complement(<1..456)
                     /locus_tag="VFY96_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605645.1"
                     /translation="MKTPAVILAIGISTGAANIAAAQTAADLNNQVTTMNNATANQGQ
                     NNVVTKISSDFSSFLGTDSKAVVTGLRNGTPITLTSTTTTPGSLPVTTTTTTMISPPT
                     GHMGFGNVYISLALAKQQLSNLGITQPTPEQLQAALTGGTITQTTGTGTT"
     gene            complement(478..909)
                     /locus_tag="VFY96_03970"
     CDS             complement(478..909)
                     /locus_tag="VFY96_03970"
                     /inference="COORDINATES: protein motif:HMM:NF023514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HEX5605646.1"
                     /translation="MAKQNTRFFMVVFAFGAMSVLNGCAAVGLTLFGVGAGVATGTSV
                     SYTLDGVAYKTFTAPLPQVENATRTALDRMGIRIEATSSIEQGKAIKALTNDRDIEIE
                     LEMVSKKTTRIRSVAKQGIFFKDRATATEIIIQTEKALGLG"
     gene            complement(1084..1737)
                     /gene="npdG"
                     /locus_tag="VFY96_03975"
     CDS             complement(1084..1737)
                     /gene="npdG"
                     /locus_tag="VFY96_03975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HEX5605647.1"
                     /translation="MEELKVAIVGGTGNLGNALALRLGAPGVKIIIGSRDAEKARNAV
                     EILRPKLRAGEMSGTTNRDAVKDADVIVIAVPYEGHAQMVTDLKGQLSGKVLIDTVVP
                     LNKVKPFVPAAGSALQEAQQILDGEAPVIGALHNISAVDLGDVDSSLGDVLVCGDDNA
                     AKQKVMDIIERIGASAYDGGPASNAYVIEGLTGVIIHLNRKYKSKHGSIRITGIKNH"
     gene            complement(1752..2504)
                     /gene="cofE"
                     /locus_tag="VFY96_03980"
     CDS             complement(1752..2504)
                     /gene="cofE"
                     /locus_tag="VFY96_03980"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEX5605648.1"
                     /translation="MKRLELIGLDGLGEIRAGDSVGDLICDTCARRQISLQDNDVLVV
                     AQKIISKAEGRVRSVDRVTPSARAFELGRKLSKDPELVEIILGESRRIVRSGGRALIV
                     ETHHGFVCANAGVDQSNVGLKQVTLLPQDADRSARQLRSAIHQRTGKRLGVIISDSFG
                     RPWRLGTVDVAIGVAGMAPIKDERGMRDRYGYELKAAVSAVADELAAAAELVMGKRDH
                     VPVVLVRGYDVQTKEDGSARELLRSDAEDLFR"
     gene            complement(2501..3190)
                     /gene="cofC"
                     /locus_tag="VFY96_03985"
     CDS             complement(2501..3190)
                     /gene="cofC"
                     /locus_tag="VFY96_03985"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEX5605649.1"
                     /translation="MQIAALIPMKGFSNAKQRLGEALSRAGREVLAEAMFRDVLRQVV
                     SARGLEATFVVTCDEHVAAIASAAGAEVIRESTEQGETSAVDFARDRLKNAGWEAVLI
                     IPGDMPLVRAEDIEMVLAQVPADARAPFALLVPSHDRLGTNGLLLAPPDLIQLRFGYN
                     SFSFHMSQLTAHRLPARFCENERIALDIDEPKDLERFLSYAMEGGETIKALRRVMAEA
                     DLWQTRSTGGA"
     gene            complement(3218..4144)
                     /gene="cofD"
                     /locus_tag="VFY96_03990"
     CDS             complement(3218..4144)
                     /gene="cofD"
                     /locus_tag="VFY96_03990"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEX5605650.1"
                     /translation="MILALTGGTGGAKLIEGLAAELDPDELTIVCNTADDTVVHGLHV
                     SPDLDTITYTLAGLIDPDQGWGLKNDTFAALQQLGRLGGETWFKLGDRDLALHIRRTQ
                     LMNDGLKLSEVTAQLRRALGVRAKILPMSDDKVQTRVNTPEGEISFQEFFVKEHWARE
                     VTGIRFSGVEQSQPAIGVLEAMSRAEGVIICPSNPITSIGPILAVPGIRSALAALDVA
                     IVGVSPLIGTTAVSGPAHKLMITCGYAPSALGVARNYADILDRLFIAVEDQNLASSIQ
                     DLSIGPVCTDIRMTTANDKRRLAAEVLASLEK"
BASE COUNT          892 a         1238 c         1147 g          978 t
ORIGIN      
        1 ggttgtgccc gtgccggtgg tctgagtgat agtgccaccg gttaaggcag cctgtaactg
       61 ctcgggggtc ggctgcgtga taccgaggtt gctcagctgc tgtttggcca gcgccaatga
      121 aatgtaaaca ttaccgaaac ccatgtgacc cgttggcgga ctaatcatcg ttgtcgttgt
      181 cgtggtgacc ggcaagctgc ctggtgtcgt tgtcgtcgat gtcagcgtga tcggcgtgcc
      241 gtttctcagg cccgtaacta ccgctttcga atccgttccc agaaacgaac tgaaatcgct
      301 gcttatctta gtaacgacgt tgttctgacc ctggttcgcc gttgcgttgt tcatggtcgt
      361 aacttgattg ttcaagtccg ctgccgtttg cgcggcagca atgtttgctg ctccagtcga
      421 gatcccgatt gccagaatta ctgctggtgt tttcatgatg ttctccccca tttatgctta
      481 gccgagacct aaagcctttt cagtctggat aataatttcc gttgcggtag cccgatcttt
      541 aaagaagatg ccctgctttg ccacgctgcg aatgcgggtg gttttcttac tcaccatctc
      601 caactcgatc tcgatgtcgc gatcgttggt gagagccttg atcgcttttc cctgctcaat
      661 actggaggtc gcttcaatcc tgatgcccat gcggtcaagc gctgtgcggg tggcgttctc
      721 gacctggggc aagggcgcag tgaatgtctt gtaagccacc ccgtccagcg tgtaagaaac
      781 tgatgtgccg gtggccacgc cggcaccgac accaaacagg gtcagtccta ccgcggcgca
      841 tccgttcaag acggacatgg cgccaaaagc gaataccacc atgaaaaatc gagtattctg
      901 ttttgccata tggccgctga ccataggcaa ttaacgagcc attatcgccg acgatattca
      961 gaggaatatt tggcgttatt tcatctgctc cagagcgagc gataacatcg ctccagacaa
     1021 aaccgtgcgg gatcagtaat atttaccggt ccaaacgagg cggggtaatt gcggcgaggc
     1081 ggctcagtga ttttttattc ctgtgatcct gatgctgccg tgctttgatt tgtatttgcg
     1141 gtttagatga atgatgacgc cggtcagccc ctcgatgaca taggcattgc tcgcggggcc
     1201 cccgtcatac gcactcgcac cgatgcgttc gatgatgtcc atgactttct gtttcgccgc
     1261 gttatcatca ccgcacacca gaacatcacc gagggacgag tcgacatcgc cgagatcgac
     1321 ggccgatata ttatgaagcg caccgatcac cggagcttca ccgtctaaaa tttgctgcgc
     1381 ttcctgcaat gcggagccgg ctgcaggcac aaagggcttc accttgttga gcggtacgac
     1441 ggtgtcgatg agaactttac cgctgagctg gcctttgaga tccgtaacca tttgcgcgtg
     1501 cccttcgtaa ggaaccgcaa taacgataac gtctgcgtct ttgaccgcgt cgcgattcgt
     1561 agtgccggac atttccccgg cgcggagttt cggccgcaga atttcgacgg cgttccgcgc
     1621 tttttcagcg tcacgcgagc cgatgatgat tttgacgccg ggtgcgccca gacgaagcgc
     1681 cagcgcgttg cccagattgc cggtgccgcc cacgattgcc actttcaatt cttccatatt
     1741 caattttcga actagcgaaa gagatcctcc gcatcggatc gaagaagttc ccgcgccgat
     1801 ccatcctcct ttgtttgcac atcatagccg cgcacgagca cgactggaac gtgatcgcgt
     1861 ttgcccatta ctaattcggc tgccgcggca agctcatccg caaccgccga aaccgccgcc
     1921 ttgagttcat atccatagcg atcacgcata cctctttcgt ccttgatcgg agccataccc
     1981 gcgacgccga tcgcgacatc gaccgtgccc agccgccatg gtctaccgaa gctgtcgctg
     2041 atgatgaccc caaggcgttt cccggtgcgc tgatgaattg cactgcgcaa ttgacgcgct
     2101 gagcgatcag catcttgcgg cagtaatgta acttgcttca aacccacatt cgattggtcg
     2161 acgccggcgt tggcacagac aaagccatgg tgggtctcga cgatcaatgc ccggccgccg
     2221 ctccgcacga tccgccggct ttcgcccagt atgatttcaa cgagttccgg atctttagac
     2281 aatttacgtc ctaattcgaa ggcgcgagcc gatggtgtta ctctatcgac agaccgtacg
     2341 cggccttccg ctttcgaaat gattttttgt gcgacgacca gtacgtcgtt gtcttgaaga
     2401 ctgatctggc gccgcgcgca ggtatcgcaa atcaaatcgc caaccgaatc accagcgcga
     2461 atctctccca gcccatcgag cccgatcagt tcaagccgct tcatgcgcca ccggtagacc
     2521 gagtttgcca gagatccgcc tcggccatca cgcggcgcag agcctttatg gtctcaccgc
     2581 cttccatcgc ataggaaaga aatcgttcga gatccttagg ttcatcgatg tccagggcaa
     2641 tgcgctcatt ttcgcaaaaa cgcgccggta accgatgcgc ggtcagttga ctcatgtgga
     2701 aactgaaact gttgtagccg aatcggagct gaatcaggtc aggcggtgcc aggagcagtc
     2761 cgttcgtacc gagccgatca tgcgagggaa cgagcaacgc gaaaggcgct cgcgcatccg
     2821 cgggaacttg cgcgagaacc atctcgatat cttcggcacg cacgagcggc atgtcgccgg
     2881 gaatgatcaa gacggcttcc caaccggcat ttttcaaccg atctcgagca aagtcgacgg
     2941 cactggtttc accctgttcg gttgattcgc gtataacttc ggcgcccgct gcggatgcga
     3001 tcgcagctac gtgttcatcg cacgtgacca cgaacgtcgc ttcgagaccg cgcgcactga
     3061 ctacctgacg caagacgtcc cggaacatcg cttccgccaa aacctcgcgc ccggcgcgac
     3121 tgagggcttc gccgaggcgt tgttttgcat tgctgaaacc cttcatcggt atgagtgcgg
     3181 cgatttgcat tccgattccg gagagaattt ccttagacta tttttccagc gaggccaaga
     3241 cctcggcggc gaggcggcgt ttgtcattgg cagtcgtcat acgaatgtcg gtgcaaaccg
     3301 ggccgataga aagatcctga atcgagctcg ccaggttctg gtcttctacc gcgataaaca
     3361 ggcgatcgag aatgtccgca taattgcgcg caacgccgag cgctgaggga gcatagccac
     3421 aagtgatcat gagtttatgc gctggcccgg aaacggccgt cgtgccgatg agcgggctca
     3481 cgccaacaat tgccacatcg agcgcagcta gagccgaacg aattcccggc acagccaaga
     3541 tcggcccaat gctggtgatc ggattgctgg gacagataat aacgccttcg gcgcggctca
     3601 ttgcttcgag cacgccgatc gcgggttggc tctgttcaac tcccgagaaa cgaattcccg
     3661 tcacctcacg agcccaatgc tccttgacaa aaaattcctg aaaggaaatt tcaccttcgg
     3721 gtgtattcac gcgcgtttga actttgtcgt cgctcatcgg cagaattttg gccctgacac
     3781 cgagggctcg acgcagttga gcggtgacct ccgacaattt tagtccgtca ttcatcaatt
     3841 gcgtacgcct gatatgcagc gcgagatctc gatcgccaag tttgaaccag gtttcgccgc
     3901 ccaaccggcc gagctgttgt aacgcggcaa acgtgtcatt tttcagaccc cagccttggt
     3961 caggatcgat caaccccgcc aatgtgtaag tgatcgtgtc gagatccggc gagacgtgaa
     4021 gcccatgaac caccgtatca tcagcggtgt tgcagacgat cgtgagctcg tcagggtcca
     4081 attcagcggc gagtccttcg atcagcttcg cacccccggt accgccggta agggccagga
     4141 tcatccgcca agtcctgggc tcgatggcat attgaaggca tcgagtgatc cgttcgtcag
     4201 cggaccattg tcgcgcaccc aaccgtcggc gccggcgagg cgctcgatgc tacgg
//
LOCUS       DASULZ010000195         2999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_24020, whole genome shotgun sequence.
ACCESSION   DASULZ010000195 DASULZ010000000
VERSION     DASULZ010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24020"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VFY96_03995"
     CDS             complement(<1..359)
                     /locus_tag="VFY96_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605651.1"
                     /translation="MIQYSPKLTFGPEVADWQERFNPERMRRQRVERAQVLMRKYGVA
                     ALLEANHHNIRYLTALKGFAYPMCRYVLFFAEHEPVMYEHDGYYHQMPDQCPWIKEWR
                     PARSWLTGAPGPDACAA"
     gene            817..1773
                     /locus_tag="VFY96_04000"
     CDS             817..1773
                     /locus_tag="VFY96_04000"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605652.1"
                     /translation="MLTMRLPWFSFVISLLISACLAAPGTAAERAPERVRIAVSSKSL
                     GFLDTWAAKERGFYRKYGLDPEIIAMRPPLTIGALQAGEIDYTFGASTISRGSISGAP
                     VRLVSLSLRTSFHTLVARPQFKSIADLRGKTIAVTIGAADDFVARHLIRRAGFEARDY
                     AFVNMGGSDTRFPALHAGSIDATALSLPFFIIARQQGFMLLGSASDVIDMATVGIGTS
                     IRKIQQEREQVKKMVQAQIDTLRWIKTQKGEVVPFLQKFFALDEATALEAHAVYARLI
                     IADARPLTEPVKTVLDQEGKPDLPIDRVVDPTIVEEVLRERR"
     gene            1776..2870
                     /locus_tag="VFY96_04005"
     CDS             1776..2870
                     /locus_tag="VFY96_04005"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5605653.1"
                     /translation="MPDLRIIDIDGHVREADDLFEKYLEAPFRDRAPRVEKVANGQLL
                     FRLEGERHHRKPDETPFRVKEDGSQVNENRHLATHPKQRLAAMDRDRIERGLLFPSAG
                     LYLPAVQEEAYAAALCRAYNNWLYDYCAADRVRLMGVAVIPVQDVKLAIDEAKRAVNQ
                     LGFKGIFVRPNPIKGRNIDDPYYDPLYRAIVDLGVALLVHEGSGAFLPTAGADRFSGQ
                     WFFTHTISHPFEQMLASLSLICRGALERHPDLQVVFLEAGAGWLPYWLWRMDEHYEVL
                     PFQVPWLKRKPSEYFRRQCFVSFEADETRLGQVIESIGADRVVFASDYPHWDATFPGV
                     TDVILAREDLSAEIKRKIMGENAARLLRLD"
BASE COUNT          630 a          856 c          835 g          678 t
ORIGIN      
        1 tcggccgcgc aagcgtccgg accaggcgcc cccgtcagcc atgaacgcgc cggccgccac
       61 tccttgatcc agggacactg gtcgggcatt tgatgataat atccgtcgtg ctcgtacatg
      121 accggttcat gctcagcgaa gaacagcacg tagcggcaca tcggataagc gaatcccttg
      181 agcgcggtca gatagcgaat attgtgatga ttggcttcga gcagcgccgc caccccatat
      241 ttccgcatca gcacctgcgc acgctcgacg cgctgccggc gcatgcgttc tgggttgaag
      301 cgttcctgcc aatcggcaac ttccggaccg aaagtaagct tcggagaata ttggatcata
      361 agagcctccg accggagagc tttccgtgcc tcgacgttat ccggaacggg ctatcggttc
      421 aacgccgctc aatgctcgcc ctgagcgcgg agcgtctgcg ttgtgcgccg ttttcacgac
      481 gcgccgagat tatcccgcag ccaagcggcg atcaagcttt cgcccaaatc cagacactca
      541 accgctgtcg gaaagatttc tcgcttgcga gactttgtga aaactcgccg ttcgaccttc
      601 gatacgctca ggatgaacgg ctaagtagtt gtcgttacag aacacctaaa ccgcgcatgc
      661 tgcgcttgtc gaagcatcag cggagttttc aaacagtttt gcgctcgata tgacagtctg
      721 ctcaattttt catctcgagc acaacggaga ttttgtttct actgctgcca ttcggtgatg
      781 cctcgcttgc aatctttcct agtgctggat acagaaatgc tgacgatgcg tttgccatgg
      841 ttcagctttg tgatttcgct gttgatcagc gcgtgcctcg ctgcgcccgg taccgcggcc
      901 gagcgtgcac ccgaaagggt ccgtatcgcg gtttcttcca aatcgctggg atttctcgat
      961 acgtgggctg cgaaggaacg tggcttttat cgtaagtacg gcctcgaccc ggagatcatc
     1021 gccatgcgcc caccgctcac aattggcgcg ctccaggcgg gagagatcga ttacaccttc
     1081 ggcgccagca ccatctcacg cggcagcatc tccggcgcgc ctgtgcgatt ggtgagtcta
     1141 tcgttgcgca cctcgtttca taccttggtt gcgcgtccgc agttcaaatc gatcgcggac
     1201 ctgagaggca aaacgatcgc ggtgaccatc ggcgccgctg acgatttcgt cgcgcgtcat
     1261 ctgatccggc gggcgggttt tgaggcgcgc gattatgcgt tcgtcaacat gggaggatcg
     1321 gacaccagat ttccggcgct tcatgccggc agtatcgatg cgactgcgtt gtctttgccg
     1381 tttttcataa tcgccagaca gcagggtttt atgcttctcg gcagcgcctc ggatgtcatc
     1441 gacatggcaa ccgtcggcat cggcacttcg atccgaaaaa ttcagcaaga gcgcgagcag
     1501 gtgaaaaaga tggtccaagc gcaaatagac actttgcgct ggattaaaac ccaaaagggc
     1561 gaggtggtgc ctttcctgca gaagtttttc gctctcgacg aagctaccgc cctggaagcg
     1621 catgccgtct atgcccggct gattattgcc gatgcgcggc cattaacaga acctgttaaa
     1681 accgtcctcg atcaggaagg caagcccgat ctgcctatcg atcgcgtggt cgatccgaca
     1741 attgtggaag aggtcttgcg cgaacggaga tgaaaatgcc ggaccttaga attatcgaca
     1801 tcgacggcca tgtgcgcgag gccgacgatc tcttcgagaa gtatttggaa gcgccgtttc
     1861 gcgatcgggc gccgcgcgtc gaaaaagttg ccaacggaca attgcttttt cgccttgaag
     1921 gcgagcgcca tcaccgaaaa cctgacgaaa ctcctttccg cgtcaaagaa gatggctctc
     1981 aggtcaatga gaatcgtcac ttggctaccc accccaagca gcgtctcgcg gcgatggatc
     2041 gcgatcggat tgagcgcggc ctgttgttcc cctcggcggg actctacttg cccgcggtgc
     2101 aagaagaagc gtatgcggct gcgctgtgtc gggcttacaa caattggctt tacgattact
     2161 gcgcggctga tcgcgtgcgg ctgatgggcg ttgccgtcat tccagtacaa gacgtgaaac
     2221 tcgccatcga tgaagcgaaa cgcgcggtca accagctcgg cttcaaagga attttcgtgc
     2281 ggccgaatcc gatcaaaggc cgcaacatcg acgatcctta ttacgacccg ctttaccgag
     2341 cgatcgtcga tctcggtgta gccctcctgg tgcatgaagg atcgggcgcg tttctgccga
     2401 ccgcaggagc ggatcgcttt tcgggacagt ggttttttac ccacacgatt tcacatcctt
     2461 tcgagcaaat gctcgcgagc ttgagcctga tttgccgcgg tgctttggaa cgccaccccg
     2521 atctacaggt cgtctttctc gaagccggcg caggctggct cccgtactgg ctctggcgta
     2581 tggacgagca ctacgaagtt ctcccctttc aagtcccgtg gcttaaaagg aaaccgagtg
     2641 aatattttcg ccggcaatgt ttcgtctcct tcgaagccga cgagacgcgc ctcgggcagg
     2701 tgatcgaatc catcggcgcc gatcgggtcg tctttgcttc cgattacccg cattgggatg
     2761 caaccttccc cggcgtcacc gatgtgatcc ttgcgcgcga agacctcagc gcggagatca
     2821 aaagaaaaat catgggcgag aatgccgcgc gattgttgag gttggactga caaagaaagc
     2881 gtttcgggtc tcaggttagt ctctcgcgtt atttttgaac tcaaaactcg gaacccggaa
     2941 ctcggaactg aaaaaccgga ggtgctatgg ccgataaacc gaaactcgtt tcatcgatg
//
LOCUS       DASULZ010000196         3644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1241203, whole genome shotgun sequence.
ACCESSION   DASULZ010000196 DASULZ010000000
VERSION     DASULZ010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241203"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VFY96_04010"
     CDS             <1..414
                     /locus_tag="VFY96_04010"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5605654.1"
                     /translation="DTVKELFEKAEGNNNEKQQKKLFEQIKTELETHTYIEETVFYPA
                     VEQIDELKDMVLESLEEHKQVKTLLREMEHLSDDSEKFEPKLKVLMENVEHHAIEEEE
                     EKMFPKLRKLMNSEQLQHLGQELEAAKNKQVRKAS"
     gene            complement(628..1476)
                     /locus_tag="VFY96_04015"
     CDS             complement(628..1476)
                     /locus_tag="VFY96_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5605655.1"
                     /translation="MPTAMLDGIATYYEIYGIGTPILMCAPGGFDATIDKWRTASAWT
                     GIDAVKSLGAEHMLILYDRRECGESGGRVERLSWPGYARHGKALLEHLKIDSAWIMGG
                     CMGCSVALGFGAVYPEATRGLLLHWPVGGYRWKVNSRNRFTRHYNFAKQNGLRAVIER
                     ARGGKSFWMDPEGGPWATEIVRTEEFAESFATQNLDRYLGIVFTTGQSLFDRDTAPGA
                     EPEEVLGVAVPALIIPGDDPSHATSGAHYLRELLPKADFWDIMPPEQTTPKVCDRLLE
                     FCRQNA"
     gene            1577..2392
                     /gene="mazG"
                     /locus_tag="VFY96_04020"
     CDS             1577..2392
                     /gene="mazG"
                     /locus_tag="VFY96_04020"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEX5605656.1"
                     /translation="MIDQHTERKFGELLEVMARLRSENGCPWDKEQTHVSLKPCLLEE
                     TYELLDALDNGDSKKLQEELGDVLLQVIFHAQIASEENRFSMKEVIEKLTEKLVRRHP
                     YVFGGEPLPEDSAAVLKQRMQIKAGEKKESGESSLLGNVPKSMPALARAQSISRRAAH
                     LGFEWPDIDRVWDKVEEEIRELKEAAASGDKARTGEELGDLLFTMVNIARFLDIESEE
                     VLAQTVDRFTRRFHHIEAKLKQANKRFEQSSLEELDQFWEEAKRIEAEQKHRS"
     gene            2389..3366
                     /locus_tag="VFY96_04025"
     CDS             2389..3366
                     /locus_tag="VFY96_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX5605657.1"
                     /translation="MSPPQTKIIFLDAATYGDVSLTHFTDLWECTIYQTTRSNETAQR
                     LAGHTLVVTNKVEIDELILGSPEARDLKLIAVAATGTDIIDRKAASAHGVRVCNVPGY
                     ATESVAQFTISLILELTTRVAKYGDAVKRGEWEKSPVFALLSYPSVELHGKKLGIIGY
                     GNIGRRVAEIARGFGMEVLIAARPGDNGPMTNDRVPINDLLREADVVSLHCPLTPETR
                     NLINHQSLSLMKPSAFLINTARGALIDEAALIDTLGKHLIGAAALDVISTEPPSSDHP
                     IIRAAKILDNLIVTPHTAWSAREARERLLNEVKENIAAFLRAEPRNLVA"
     gene            complement(3388..>3644)
                     /locus_tag="VFY96_04030"
     CDS             complement(3388..>3644)
                     /locus_tag="VFY96_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005455770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HEX5605658.1"
                     /translation="ARFVLNVHGRWDEAKDDRICIDWARDFFKASAPYASAGAYVNFM
                     TAEEGDRIASAYGSNYVRLIEVKRKYDPDNTFHLNQNIKP"
BASE COUNT          921 a          973 c          991 g          759 t
ORIGIN      
        1 gacacggtaa aagagttatt cgaaaaggca gaaggcaata acaacgagaa acaacaaaaa
       61 aaactgttcg agcagatcaa aacggagctg gaaactcaca catatattga ggaaaccgtt
      121 ttttatccgg cggtggaaca gattgatgag ctgaaagaca tggtgctcga atcactagaa
      181 gagcacaagc aggttaagac tttgctccgt gagatggaac accttagcga tgatagcgaa
      241 aagtttgagc ccaagctcaa agtcttgatg gaaaacgtcg agcatcacgc gatcgaagaa
      301 gaagaggaga aaatgtttcc caagctacgc aaattgatga acagcgagca gctccaacac
      361 ctaggtcaag agctggaagc ggcgaagaat aaacaagtac gtaaagcgag ctgacgaatt
      421 tcactcctat atgcgcatcg atttgacgac tcggcttgcc gctgccacgg cggcaagccg
      481 gccagtcaaa tatttcactg gcagcgtttc aaaaatagac atgccaaagt cgattccgtt
      541 ctggatctgt cctcacctga agccgactcg aagccttaca gggttcgatg agatggtcca
      601 agcgcgccga agcctgatga atttgcacta ggcattctgc cggcaaaact ccagcagtcg
      661 gtcacaaact ttcggcgtgg tctgttcagg cggcatgatg tcccagaaat ctgcttttgg
      721 gagcagctcg cgtagatagt gcgcgcccga agtggcatgg gacggatcat caccgggaat
      781 aattaacgct ggtacggcaa cgcccagtac ttcttccggc tcggcgccgg gcgcggtatc
      841 gcgatcaaag aggctctggc cggtggtaaa aacgatgccc aggtatcgat ccaggttctg
      901 agtcgcaaaa ctctccgcaa actcttcagt ccgtacgatc tccgtcgccc acggccctcc
      961 ttccggatcc atccaaaaac tcttgccacc gcgtgctcgc tcgatcacgg cgcgaaggcc
     1021 attttgcttg gcaaaattgt agtggcgcgt aaagcggttg cggctgttta ctttccatcg
     1081 gtaaccacca accggccagt gtagtaaaag gccgcgcgtc gcctcggggt aaactgcgcc
     1141 aaaacctagt gcgaccgagc agcccatgca accacccatg atccatgccg aatcgatctt
     1201 taaatgctcc agcagcgcct tgccatgccg cgcgtaaccg ggccaactga gccgttcgac
     1261 gcggccgccg gattcgccgc attcgcgccg gtcgtagaga atcagcatat gttcggcgcc
     1321 caacgatttc actgcatcga tgcctgtcca ggcgctggcg gtgcgccact tgtcgatggt
     1381 cgcatcaaag ccgcccggag cgcacatcag gatcggcgtg cctatgccgt aaatttcata
     1441 ataggtagcg attccgtcga gcattgcagt tggcatttgc cgttctcctt gtctgctgga
     1501 cttaaaaaca tgcggcacgt aagatttaac acggtgccgg tgttcagaaa aggcgcagcc
     1561 gacgtggagg cgaacagtga ttgatcaaca caccgaacgg aaattcggcg aattgctcga
     1621 agtgatggcg cgtttgcgca gtgaaaatgg ctgtccgtgg gacaaggagc aaacgcatgt
     1681 ctcattgaag ccctgtttat tggaagaaac ttacgagctg ctggatgccc tggacaatgg
     1741 tgactcgaaa aaattgcagg aagaactggg ggacgtccta ctgcaagtca tttttcacgc
     1801 gcagattgcc agcgaagaaa accgtttctc gatgaaagag gtgattgaaa aattgacgga
     1861 gaagctcgtc cgccgccacc cctacgtatt tggcggcgaa ccgttgccgg aagactccgc
     1921 agcggtgttg aagcagcgca tgcagatcaa agctggcgag aagaaggaga gcggcgaaag
     1981 ctcgctcctg ggaaacgtgc ccaaaagcat gccggcgttg gctcgggcgc aatcgatctc
     2041 gcggcgcgct gcgcatctcg ggttcgagtg gccggacatt gacagggttt gggacaaggt
     2101 agaagaggaa atccgtgagc tcaaggaagc cgccgccagc ggagacaaag ctcggacggg
     2161 tgaagagctc ggcgatctcc tttttaccat ggttaacatt gcgcgttttc tggacatcga
     2221 atcggaagag gtcttggcgc aaaccgtcga tcgcttcacc cgccgctttc accacatcga
     2281 agcgaaactg aaacaagcaa acaagcgctt tgagcagtct tctctcgaag aactagatca
     2341 attttgggaa gaggcgaaga gaatcgaagc tgagcaaaag caccggtcat gagcccaccc
     2401 caaaccaaaa tcattttttt ggatgccgca acatacggcg acgtctcgct cacccatttc
     2461 accgaccttt gggagtgcac gatttatcag accacgagat cgaatgaaac ggcgcaaagg
     2521 ctcgcgggcc ataccctagt ggttacgaat aaagtcgaaa tcgatgagct gattctgggg
     2581 tcgcctgaag cgcgcgactt aaagctgatc gcggttgccg ccacaggtac ggatattatc
     2641 gaccggaagg cagcttcggc tcatggtgtg agagtttgca acgtgccggg ttacgctact
     2701 gaatccgtag cgcagttcac gatttccctg attctggaat tgaccacccg tgttgcgaaa
     2761 tatggtgacg cggtgaaacg tggcgaatgg gaaaagagcc ccgtttttgc tttgctcagc
     2821 tatccctcgg tggagctcca cggaaaaaaa cttggcatca ttggctatgg caatatcggc
     2881 cgccgggttg cggaaatcgc gcgcggattc ggtatggaag ttcttatcgc cgcgcgtcca
     2941 ggagacaatg gaccgatgac caacgaccgc gttccgataa acgatctgct tcgcgaagcc
     3001 gacgtcgttt cacttcactg tccgctgacg ccggaaacca gaaacttgat caaccatcag
     3061 tccttgtcgc tcatgaaacc gtcggccttc ttgatcaaca ccgcgcgcgg cgcgctcatc
     3121 gacgaagccg cgctcatcga tacgttaggt aaacacctga tcggcgccgc cgcgctcgat
     3181 gtcattagca cagagccgcc ctcatctgac catccgatta tccgcgcggc gaaaattttg
     3241 gacaatttga tcgtcacgcc gcatacggcc tggagcgcgc gcgaagcgcg cgagcggctc
     3301 ctcaatgagg tgaaggaaaa tatcgcggcc tttctccgcg ccgagccgag aaacctcgtc
     3361 gcgtgaaagg ccgccggaga ccgcgcttta tggttttata ttctggttta aatgaaaagt
     3421 gttatccgga tcatattttc ttttgacttc gattagccga acgtagttgg agccgtaagc
     3481 actagcgatc cgatcgcctt cctctgccgt cataaaattc acgtaggctc cagccgatgc
     3541 ataaggcgcg ctggctttaa agaaatcacg cgcccaatcg atgcatatcc gatcatcttt
     3601 cgcttcatcc cagcgaccgt gtacgttcaa aacgaacctt gcgt
//
LOCUS       DASULZ010000197         9251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_633075, whole genome shotgun sequence.
ACCESSION   DASULZ010000197 DASULZ010000000
VERSION     DASULZ010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9251
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633075"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VFY96_04035"
     CDS             complement(<1..754)
                     /locus_tag="VFY96_04035"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605659.1"
                     /translation="MSSLFPGELKRFIVFAALFLSIPVFCVRTAAAQAKQPCKEMRKV
                     KLGVSVAPPNVVHTPVYVARDLGIFANHCIEAEIIEFEGGLSATNLVAVAKGGTMASM
                     NATAIAQGMTAKQIWGMAPRLPQAYVVSEDVKTAADLKGKRLSAAGGGVGSFNWVMGR
                     EVLKTAGLKVEDVQFISQGTAGRLPGLVAGQLNGVALHPEDVYLAQKEKPGVHVLVQL
                     VDLMPKYFFNAYGAGDSQLARERTLVRDTVTAL"
     gene            complement(773..1648)
                     /locus_tag="VFY96_04040"
     CDS             complement(773..1648)
                     /locus_tag="VFY96_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5605660.1"
                     /translation="MSVTQAESRVQPNSPYSTDSDISAEQQAARDRAKAQQRKSMAAN
                     LGIRIVSLVIVLGGWEYFGRQVNPVLFTYPTAVGAAAIKMIGSGELWQYLSQSLIVLG
                     AGLALAVVFGIALGVIMARYWVIDIALDTYITALYSIPSVALVPVLVLWIGFETSAKI
                     AVVFLFTFFPMVINTYQGVKNVDTRLLEVGRAFRCSERQLWANIILPAALPFIVAGLR
                     LSIGRGLIGMVLADLYTAITGIGYLISRYASIYRTDAMFVPVVTLGVLGITLTGLLRV
                     IERWVTPWMHYSDRD"
     gene            complement(1629..2423)
                     /locus_tag="VFY96_04045"
     CDS             complement(1629..2423)
                     /locus_tag="VFY96_04045"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015791850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5605661.1"
                     /translation="MPDGVKIIVDHASKLFLEGSVVAFRNIDLEIRSKEVLCVVGPSG
                     CGKTTLLRCIGGLLAPSSGKVLIDGQAVDRPREGVAIVFQHFGLLPWKSVAENVAFGL
                     QMAGASRQLIKERTDRYTKLVGLENFEAQYPYQLSGGMQQRVGLARALAINPDILLMD
                     EPFASVDAQTREVLQEELLQLHDRERRTMLFITHSIDEAIVLGDRIAVMTTRPGRVKE
                     ILPIDFKRPRDPAAVRSHPHYGELRNHIWNELRPTVLEGAYERHSS"
     gene            complement(2621..3616)
                     /locus_tag="VFY96_04050"
     CDS             complement(2621..3616)
                     /locus_tag="VFY96_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5605662.1"
                     /translation="MRLNRQNNIFLVLLILLLPAVKVSGQEKYPDRPITIVAHFPPGG
                     VADLTARPVAAALEKVLKNPVVVVNKTGAAGAVGMSFVANSKPDGYNLLMALSSISII
                     PEADKLFDRKPAYTMDQLLPIALISADPTIFVVNASRPWKTVKEFVDDAKKRPGEISY
                     SSSGVYGTLHMAMEMLIHGAGIQLKHVPYSGAGPALTALLGGHVDTLASGPAVVIPHI
                     KAGKLRPLAGWGAKRVASLPDVATFKELGYDIEFYIWAGLFAPRGTSPAVMKTIREAV
                     KQAVNSVEFKTAMEKIETPIAYLDAPEFQKFWDKDAKMLADAIKRIGKIEIPEAK"
     gene            complement(3642..5177)
                     /locus_tag="VFY96_04055"
     CDS             complement(3642..5177)
                     /locus_tag="VFY96_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX5605663.1"
                     /translation="MADTLHNLYIGFSVALAPQVLIYAFVGCVVGTLVGVLPGIGPLA
                     GISLLLPASFGLDATSAIVLLAGIYYGAMYGGSTTSILMRIPGEAASVMTCIDGYEMT
                     RRGRAGPALAIAAIGSYIAGTVSVVGLMLLAPPLASFALRFGPPEYLALLLLGLLVLA
                     YMSGGSMLKSLAMAALGLLLGMIGIDPMTGFFRFSYGLVELGDGIGVVPVAVGLFGLS
                     EILLTAGQPTAPEVKKPRLKDLLPSKQEWRDSLWPIGRGTVLGFLIGIIPGSAHIISS
                     FVSYAVERRLAKDPERFGHGAIEGVAGPESANNSATSGAFVPMLALGIPSGPIPAVMI
                     AAMMVHGISPGPLLITQQPELFWGFIASMYVGNVVLLILNLPLVGIFVNVLRVPYPLL
                     YPAILLFCVLGVYAVNGSTVDIGIMAGMGALGYLLRKFDFETAPVVLGLILAPMIEMS
                     LRQSLAMSSGSYAIFVNRPIASVMLLIGLALLLLSLMPFLMKSIDWRQRLGINTANEG
                     EKL"
     gene            complement(5237..5677)
                     /locus_tag="VFY96_04060"
     CDS             complement(5237..5677)
                     /locus_tag="VFY96_04060"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX5605664.1"
                     /translation="MTTDRLSAGVLALFALFVMWESRQLPLGTFRHPGPAYVPVLLAS
                     LLLLFAIFILFTASRAPKLSSLQWTEWRHALAIFACSVFSVFAIERLGYRLTMLLMLA
                     FLVKLVEKRGWIVSLIFSFSLSWGSYFLFYTLLRVPLPQGPLGF"
     gene            complement(5747..6898)
                     /locus_tag="VFY96_04065"
     CDS             complement(5747..6898)
                     /locus_tag="VFY96_04065"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEX5605665.1"
                     /translation="MKFSTERILTTHAGALPQPSELKQMHGARDTGTPLNADAFRHRV
                     RAAVAEVVKKQLECGLDIINDGEVGKSNFSRYARDRLSGFIERNAKPGEQTSTIFARD
                     LLEFGEYFKNRTYHRGDNVRRVFCNAPLKYVGYDSLKSEIEDFKAALAGQKYEEAFLP
                     AIAPGTIEHWMKNDHYPNDEAYLMAIADAMHEEYKAIVDAGFLLQIDDPDLPDGWQFM
                     SNMTVPEYRNYAELRIDALNHGLHDIPPDRVRFHTCWGSYHGPHKYDIPLRDIIDLIL
                     KVKANTISIEAANPRHEHEWRVWEEVKLPAGKVLVPGVVGHATDIVEHPQAIADRLVR
                     YAKIIGRENVMAGTDCGLGPRVGDPQIAWAKFEAMSEGARIATKELWKR"
     gene            complement(7031..8107)
                     /locus_tag="VFY96_04070"
     CDS             complement(7031..8107)
                     /locus_tag="VFY96_04070"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605666.1"
                     /translation="MPNGVDFCFHSCRVTARPDSHIAVGLCKSIRAVRLAFFGLAASA
                     LVAFPVSEIRAAEKSAKLVPLRIGYVSRSILDMPFIIARDRGFFREEGFDAELIFINA
                     IQSVQAVIAGSVDFGTATGSGISAAVNGADVRVVFGLTDKPSFDLFALPNITSIQQLR
                     GKKIGVSAPGSLTEILARQILLANKIPLDQVTLLPLGTSNVTYVALKAGTIEATMLQI
                     PQNFLAIDEGFRKLAAGADVYRALQGGLTTSKATIMERPDVVTKTIRATQRALRLIRR
                     DRKLAVDFIKGPFLELGKERDRFAERIYDAAVQLYLESGVVDEKIQREMIATASQRVK
                     PKEPVPPERVFDFSFAQKVADALK"
     gene            complement(8100..8768)
                     /locus_tag="VFY96_04075"
     CDS             complement(8100..8768)
                     /locus_tag="VFY96_04075"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX5605667.1"
                     /translation="MGNEAFVESVLQEIIRPGVERLMSSRYFSELREGKLSKKRLKGF
                     ALQHYLSNHAINKGLAFCMVKNASNPPAYNQFVELFVEESSHPALMKRFGEALGLTDA
                     DFENEIMIFECVAHTAAIIRGMFLGSAAETRAGALVNETMVQRYSEEFDTALAKHYGL
                     DSRARTFFAVHGKVDQEHTAMAAETIARNANTDREKELVRVAAKNMVRFKLAKFDGIY
                     DAYA"
     gene            8914..>9251
                     /locus_tag="VFY96_04080"
     CDS             8914..>9251
                     /locus_tag="VFY96_04080"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5605668.1"
                     /translation="MPGALDGIKVLDVTNYIAGPFATMLLADLGAEVYKIETPGGGDP
                     FRTWDKEPKDYSPSFCGMNRNKKSITLDIKQPEGRKIFMRLAEGADVIVENLRPGVVD
                     RLGIGYEEIK"
BASE COUNT         2152 a         2556 c         2658 g         1885 t
ORIGIN      
        1 tcaaagcagt cacggtatca cgtaccagcg tccgctcgcg cgcaagttgg ctgtcgccgg
       61 cgccataagc attgaaaaaa tacttcggca tcaggtcgac gagttgaacc aatacgtgga
      121 caccgggctt ttctttttgt gcgaggtaga cgtcctcggg atggagcgcc acgccgttta
      181 gctggcccgc gaccagtccg ggcagacggc cggccgtgcc ctgagagatg aattgcacgt
      241 cttcgacctt gagtccggcc gttttcagta cttcacgtcc catgacccaa ttgaagctgc
      301 ccactccgcc gccggctgcg ctgagacgct tgcccttcaa atcggcggcg gttttaacat
      361 cttcactgac gacgtaagcc tgtgggaggc ggggcgccat gccccaaatc tgcttggcgg
      421 tcattccttg cgcgatggcc gtggcgttca tcgacgccat ggttccgccc ttagcgaccg
      481 cgacgaggtt ggtggcggac aagccgcctt cgaactcaat aatttcagct tcgatgcaat
      541 ggtttgcgaa gatgccaaga tcacgcgcga cgtagacggg agtatgaaca acgttgggcg
      601 gagccaccga gacgccgagc ttcaccttac gcatttcctt gcagggttgt tttgcttggg
      661 ccgctgccgt tcggacacaa aacacgggga tcgaaaggaa tagcgctgca aaaactatga
      721 agcgcttcaa ctcgccggga aataagctgc tcatcttggc ctccgttcct attcaatctc
      781 tatccgaata atgcatccaa ggtgtaaccc accgctcgat tacgcgcagc agaccggtca
      841 gagtgatccc caacacaccc agcgtgacga ccggcacaaa catggcatcg gttcgataga
      901 tactcgcata acgagaaatg agatagccga tgcccgtgat cgccgtgtag agatccgcca
      961 gcaccatccc gatcaggcca cggccgatgg acaaccggag cccggcgacg ataaaaggca
     1021 gcgcggcggg taaaataatg ttggcccaga gctggcgctc gctacagcga aacgcgcgcc
     1081 cgacctcgag caagcgcgta tcgacgtttt tgacgccttg ataggtattg atgaccatcg
     1141 ggaagaacgt gaataaaaaa accacggcaa tctttgccga ggtttcgaag ccgatccaga
     1201 gtaccaggac gggcaccaac gccaccgacg gaatcgagta aagcgccgtg atgtaagtgt
     1261 cgagcgcaat gtcgatcacc caatagcgcg ccatgatcac acccaaggcg atgccgaaga
     1321 cgacggcaag agccagtcca gcgccaagca cgatcaagct ttgtgagagg tactgccaca
     1381 gctcaccgct gccgatcatc ttgattgccg ccgcgccgac cgccgttggg taggtgaaga
     1441 gaaccgggtt cacttgacgt ccgaagtatt cccagccgcc gagcacgatg acaagcgaaa
     1501 cgatgcgaat cccgagattg gctgccatcg actttcgctg ctgcgctttt gcccggtccc
     1561 gcgcggcttg ctgttcggcg ctgatatcgg aatccgtcga atacggggag ttgggctgta
     1621 ctctgctttc agcttgagtg acgctcatag gctccctcga gaactgtggg tcttagctca
     1681 ttccaaatat gattgcgtag ctcaccgtaa tgcggatgag agcgcacggc ggccggatcg
     1741 cgtggacgtt tgaaatcgat cggcaggatt tccttcacgc ggccgggccg agtcgtcatt
     1801 accgcaatgc gatcgccgag cacgatggct tcatcgatcg agtgagtaat gaacagcatg
     1861 gttcggcgct ctctatcgtg tagctggagc agttcttcct gcagcacttc gcgcgtttgc
     1921 gcatccacgg aagcgaaggg ctcgtccatc aataaaatat ccgggttgat cgcgagcgcc
     1981 cgcgccaagc cgactctctg ctgcatgcct ccgctaagtt ggtagggata ctgagcttcg
     2041 aagttctcca gcccgacgag cttggtgtaa cggtcagttc tttcctttat gagttggcgc
     2101 gacgcgcccg ccatttgcag gccgaaggcg acattctccg cgacgctttt ccatgggagc
     2161 agaccgaagt gctggaagac gatggcgacg ccttcgcgcg gccgatcgac ggcttgcccg
     2221 tcgatcagaa ctttgccgga actcggcgcc aacaggccgc cgatgcagcg cagcagcgtc
     2281 gtcttgccgc aaccggacgg ccctacgaca caaagaactt ctttgcttct tatttctaga
     2341 tcgatattgc ggaaggcaac gaccgatccc tcgaggaaaa gtttactggc gtgatcgacg
     2401 atgatcttta cgccgtctgg catagcagga ggttaacgct tttccgcgtc gtgattctag
     2461 agtttcgcaa tcaaagtttg ttgcgtgtcg tccaggcaaa atttcccctt gccgctttgc
     2521 aaagaggggt taaagcaacg acgaacaaac ttcccctttg aaaaagtggg atgtaagggg
     2581 gcatttaacc gacaatagac tcaccgtcat ctgcactagc tcacttggct tccgggatct
     2641 cgattttgcc gatgcgtttg atggcgtcag ccagcatctt tgcgtcttta tcccagaact
     2701 tttgaaattc gggagcatcg agataggcga tcggcgtctc gattttttcc atcgcggttt
     2761 tgaactcgac gctgttgacc gcctgtttca ccgcttcgcg gatggttttc atcacggccg
     2821 gcgacgttcc acggggcgca aacagtcccg cccaaatgta aaactcaata tcgtagccaa
     2881 gctccttgaa ggtagcgacg tccggcagcg acgcaacccg tttcgcgccc cagccggcga
     2941 gcggccgtag ttttccagct ttaatatgtg ggatcaccac cgcgggaccg gaagcgagcg
     3001 tgtcgacgtg gccgcccagg agcgcggtga gcgccggacc tgcacctgaa tatgggacgt
     3061 gcttgagctg gatacccgcg ccgtggataa gcatctccat ggccatgtgc aaagtgccgt
     3121 agacgccgga ggaagagtaa gaaatttcgc ccgggcgctt tttggcgtcg tcgacaaatt
     3181 ctttcacggt tttccacggc cggctcgcgt tgaccacgaa gatcgttgga tccgcggaaa
     3241 tcaaggcgat cggaagaagc tggtccatgg tataggcggg tttgcggtcg aagagcttgt
     3301 cggcttcggg aatgatcgag atgctcgaca gcgccattag caagttgtag ccgtcgggtt
     3361 tgctattggc gacgaacgac atacccacgg cgcccgcggc gccagtcttg ttcaccacga
     3421 cgacggggtt cttcagcacc ttctcgagcg ctgcggccac aggccgcgct gtaagatcag
     3481 cgacgcctcc gggtggaaag tgagccacga tcgtaatcgg gcgatcggga tatttctctt
     3541 ggccggaaac cttgaccgcc ggcaggagca gaattaacaa cacgagaaaa atattattct
     3601 gtcggttcaa tcgcatcact cacctccgat gagtccgccc atcaaagttt ttctccctcg
     3661 ttggcagtgt ttatgcccag tctctggcgc caatctatag atttcataag gaatggcatg
     3721 agacttaaca gtaagagcgc gagtccgatc agcagcatca ccgatgcgat tggccggtta
     3781 acgaagatcg cgtaacttcc cgaggacatt gccagtgact ggcgcaagct catctcgatc
     3841 attggcgcga gtatcagccc caatactacc ggtgcggtct cgaagtcaaa cttgcgcagc
     3901 aggtaaccga gcgcgcccat ccctgccatg atgccgatgt cgacggtgct gccgttgacc
     3961 gcgtaaacac cgaggacgca aaagagcagg attgcaggat acaaaagtgg atagggaact
     4021 cggagcacgt tgacgaaaat gcctaccagc gggagattca agatcagcag cacgacgttg
     4081 ccgacataca tgctggcgat gaagccccag aaaagctcgg gctgctgagt gatcagcagc
     4141 ggtcccggcg aaatcccgtg caccatcatc gcggcgatca tcaccgcggg gatcggtccg
     4201 gaaggaatgc cgagggcaag catcggaaca aaagcgccag aggtcgcgga gttattcgcc
     4261 gactccggcc cggctacccc ttcaatggcg ccgtgaccaa agcgctcagg atctttggca
     4321 aggcgacgct ccactgcgta ggaaacaaac gacgagatga tgtgcgcgga gccgggaata
     4381 atgccgatca aaaaaccgag cacagtaccc cggccgatgg gccagagtga atcgcgccat
     4441 tcctgtttcg acggaaggag gtctttcaac cttggttttt tgacctcggg cgccgtgggc
     4501 tgcccggcag tcaaaagaat ttcagagagg ccgaacagtc cgaccgcgac cggcacgacg
     4561 ccgatgccat cgccgagttc caccaagcca tagctaaaac gaaagaagcc ggtcatcgga
     4621 tcgataccga tcatgcccag gagaagaccg agcgcggcca ttgccagaga tttgagcata
     4681 gagccaccgc tcatgtacgc gagcactaag agaccgagca ggaggagggc taaatactcg
     4741 ggcggtccga agcggagcgc aaagctcgcc agaggcggcg cgaggagcat cagtccgacg
     4801 acgctgacgg tgccagcgat gtacgatccg atggcggcga tcgccaaggc aggtccggcg
     4861 cggcctctgc gcgtcatttc gtagccgtcg atacaggtca tgacagaagc ggcctcgccc
     4921 gggatgcgca tcagaattga tgtagtcgat ccgccataca tcgcgccgta gtaaatgccg
     4981 gccaaaagca caatggcgga ggttgcatca agtccgaagg aagcgggaag gagcaagctg
     5041 atgccggcga gcgggccgat gcccggcaaa acaccgacga gtgtgccgac cacgcagccg
     5101 acaaaagcgt agatcagaac ctgcggcgcg agcgcgacgg aaaagccaat gtagagattg
     5161 tgcagcgtgt cagccaaagg cgttccaatc gttcaagccg ttcaaaacgt tcagacagtt
     5221 caaggtaaac gtcccttcaa aatcccaacg gcccttgcgg tagaggcacg cgcaacagcg
     5281 tataaaaaag aaaatatgag ccccaggaca agctaaagga aaatatgagc gagactatcc
     5341 agccgcgttt ttccacgagc ttgactagaa acgcgagcat cagaagcatc gtgagacggt
     5401 aaccgagacg ctcgatggcg aacacggaga agacggagca ggcaaaaatt gctagcgcgt
     5461 gacgccactc ggtccactgg agcgaagaca gcttgggcgc tcggctagcg gtgaacaaaa
     5521 taaagattgc aaagagcagc aggagcgatg ctaggaggac gggaacataa gcggggccag
     5581 gatggcgaaa agttccgagc ggaagctggc ggctctccca catcacaaac agggcaaaga
     5641 gcgcgagcac gccagcggac aatctgtcgg tggtcatggc gggcggattc tagcagcgcg
     5701 ggccgggagc acaccagcaa aattaaagag cttaattatc taacaactat cgtttccaaa
     5761 gctctttggt ggcaatgcgc gcaccttcgg acatcgcctc aaacttcgcc caggcgatct
     5821 gcggatcgcc cacgcgcggt ccgagaccac agtccgtacc ggccatgacg ttttcacgtc
     5881 cgatgatttt cgcatagcgt accaaacggt cggcgattgc ttgcgggtgc tccacgatat
     5941 ccgtcgcatg cccgacgacc cccggaacga gcacctttcc ggccgggagc ttcacttcct
     6001 cccagacacg ccattcgtgc tcgtgacggg gattggccgc ttcgatggag atcgtgttcg
     6061 ctttgacctt gagaatgagg tcgatgatgt cgcgcagcgg aatgtcgtat ttgtgcgggc
     6121 cgtgataact gccccaacag gtatgaaagc gaactcggtc gggcgggatg tcgtgcagcc
     6181 catgattgag ggcgtcgata cggagctcgg catagttacg gtattcaggg accgtcatgt
     6241 tggacatgaa ttgccaaccg tcgggcagat ccggatcgtc gatctgcaac agaaaaccgg
     6301 catcgacaat ggccttgtat tcctcatgca tcgcatcggc gatcgccatc aggtaggctt
     6361 cgtcgttggg gtaatggtcg ttcttcatcc aatgctcgat ggtgcccggt gcaatggcgg
     6421 gaagaaatgc ttcttcgtat ttttgtcccg caagagcggc tttgaagtct tcgatttcgc
     6481 tcttgagcga gtcatacccg acgtacttca gcggggcatt gcagaaaacg cgtcgtacat
     6541 tgtcgccacg gtgataagtt cggtttttaa agtactcgcc aaactcgagc agatcgcggg
     6601 caaagatcgt cgaggtctgc tcgccgggtt tggcgtttcg ttcgatgaag ccgctcaaac
     6661 gatcgcgcgc ataccgggaa aagttggatt ttccgacttc gccgtcattg atgatatcga
     6721 ggccgcactc gagctgtttt ttgacgactt cggcaactgc cgcgcgaacc ctgtggcgaa
     6781 acgcgtccgc gttcaacgga gtacccgtat ctcgagcgcc gtgcatctgt tttaactcgg
     6841 acggttgcgg caaagccccg gcgtgagtgg tcaaaattcg ctcggtgcta aacttcatct
     6901 cttctcatcc tccgtatctt cgattgtcat tttgaggcgc agccgagaaa tctttctgaa
     6961 acgggtaaaa agctagatcc ctcacattcg ttcgagatga cagcattaaa aaattgcggc
     7021 atcgcagcaa tcatttcagg gcgtcggcga ctttctgggc gaagctaaaa tcaaaaaccc
     7081 gctcgggcgg gaccggctct ttaggtttga cccgctgcga agcggtggca atcatctcac
     7141 gctgaatttt ttcgtcgaca acgcctgatt ccaaataaag ttgcacggca gcatcgtaaa
     7201 tgcgctcggc gaaccggtcg cgctctttac ccagctcgag aaacggccct ttgatgaaat
     7261 caaccgcgag ttttcgatcg cgtcggatca ggcgcaaggc gcgctgtgta gcgcgaatcg
     7321 tcttggtgac aacatcgggg cgctccatga tcgtcgcttt cgatgtggtc aggccacctt
     7381 gcaaggcgcg atagacatca gcgccagcgg cgagcttgcg aaaaccttca tcgatggcaa
     7441 gaaaattctg cggaatctgc aacatcgtcg cttcgatcgt acccgcttta agagccacat
     7501 acgtcacatt actcgtgccg agcggaagca aggtcacctg gtcgagggga attttattag
     7561 cgagcaagat ttgacgggcg aggatttcgg tcaacgatcc gggagcggag acgccaattt
     7621 tttttccgcg caattgttgg atgctcgtga tatttggcag ggcaaataga tcgaaagaag
     7681 gtttgtcggt caacccgaaa accactcgca catctgcgcc attgacagca gcgctaatgc
     7741 cggacccggt ggccgtgcca aaatcgacgc taccggcgat cacggcttgg acgctttgga
     7801 ttgcgttgat gaatatgagc tcggcgtcaa aaccctcttc acgaaaaaaa ccccgatcac
     7861 gcgcaatgat gaaaggcatg tccaaaatgc tccgcgacac gtagccaatg cggagcggta
     7921 cgagcttggc agatttttct gcggcgcgga tttcgctaac gggaaatgcg accagagcgg
     7981 acgcggcgag tccgaagaac gcaaggcgca cggcacggat cgatttgcat agccctacgg
     8041 cgatgtgtga gtccggtcga gccgtgacgc ggcaggagtg aaaacagaaa tccactccgt
     8101 taggcatacg cgtcatagat gccgtcgaac ttggccagtt tgaagcgtac catgttcttg
     8161 gcggcgaccc ggacgagttc cttttctcgg tcggtattgg cattacgcgc aatcgtttcc
     8221 gcggccatgg cggtgtgttc ttgatcgacc ttgccgtgga cggcgaaaaa ggtgcgcgcc
     8281 ctgctatcga ggccgtagtg ctttgccaac gcggtatcga attcttctga atagcgctgc
     8341 accatggtct cgttcacgag cgcaccagcg cgggtttccg cggcggagcc gagaaacatg
     8401 ccgcgaatga tcgcggcggt atgggcgacg cactcgaaaa tcatgatttc gttctcgaaa
     8461 tctgcatcgg tcaagccgag cgcctcaccg aatcgcttca tcagcgccgg gtgagaagat
     8521 tcctccacga acaattcgac gaattgattg tacgccggtg gattgctggc gtttttgacc
     8581 atacaaaagg cgagaccttt gttgatcgcg tggttcgaga gatagtgctg gagcgcaaag
     8641 cccttgagcc gcttcttgga aagcttacct tcgcgcaact cgctaaaata gcgcgagctc
     8701 atcaagcgct cgacaccggg ccggataatc tcctgcagca ccgattcaac aaatgcttcg
     8761 tttcccatac agctcctcga atgtaggcca atgcacagga acattctcag agcggaaaag
     8821 gcctgtcaag ggtgaatccg aaaccgacac gcgcgggttt agttgacagt cataggtgac
     8881 gctccctata gtccggagca tctggaggaa tccatgcccg gagcgctcga cggaatcaaa
     8941 gttcttgatg tcaccaatta tatcgccggt ccgtttgcga cgatgttgct cgcggatctc
     9001 ggtgccgaag tttacaagat cgaaactccc ggaggaggag atccctttcg cacctgggac
     9061 aaggagccca aggattacag cccgagcttt tgcggcatga accgcaacaa aaagagcatt
     9121 actctcgata tcaaacagcc ggagggaaga aaaattttta tgcggctcgc cgaaggggcc
     9181 gacgtgatcg tcgagaatct tcgacccggc gtcgttgatc gtttgggcat cggctatgag
     9241 gagatcaaga a
//
LOCUS       DASULZ010000198         3868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1038325, whole genome shotgun sequence.
ACCESSION   DASULZ010000198 DASULZ010000000
VERSION     DASULZ010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038325"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VFY96_04085"
     CDS             <1..636
                     /locus_tag="VFY96_04085"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX5605669.1"
                     /translation="EKFGRNPALLIIDVITGFTGTKPMDVMDAIDEFPTSCGQVAWDA
                     LPKIKELLHACRDTGVPVIYSTSDPDFKAAFGNATKRGVDKTDFEKIAVEFPDMIKPR
                     DDEFIVRKARASAFFGTHLITYLVRKNIDCLLVAGTSTCGCVRGTVLDGYSFGYPVFV
                     VEECVFDRSRTSHLVNLFEMNAKYASVIQLAEALDYVNKLQRMEGRKAIAS"
     gene            660..1796
                     /locus_tag="VFY96_04090"
     CDS             660..1796
                     /locus_tag="VFY96_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX5605670.1"
                     /translation="MATEAPFPEEEYLARYQRAQALMDRDGVDALLVSEKNNYWYFSG
                     LISYQLDHIQRPQILILPKTGKPLLLVYGNDKAKAKALPWVGEVRAYTDVPFPEEMIA
                     GCIKEMGLGQAKIGFELGDDQRLGFPVNYLTRLTEALPQAEIRDGTVALTEMRILKSP
                     REIEFMRKACEISVKAYDRCLPQLKPGMTRREVADRLYISMIEEGAHPRHPGFLMLNA
                     STRYDDRRYNQGDRMIADFGACYEGYYGDVTRMAIFGAPTDEQRRDHETACDVIDRCF
                     ESMQPGTPLAEASRVANRELIKRGYEAVESPKRIGHGIGMSRAEPPSLNEVETEIYRP
                     GMVLALEPKVRSAKSAVHLEEDILITDKGPEFLTAGREILSVID"
     gene            1927..2922
                     /locus_tag="VFY96_04095"
     CDS             1927..2922
                     /locus_tag="VFY96_04095"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX5605671.1"
                     /translation="MAANGILYLSNEDVKNVLDLGRAIEITQQALRDHSAGRVIWSTP
                     EDLAIKPESGWQSWVTGCALVTEPVAGFRIRSIKAAGGSRDASRAPRGPRRILILSDR
                     EGGEIRAIMDEDWCHAVRTAAAATVAMRLLARKDSAVMTMLGAGDTARAAVPVMAQAF
                     RLKEIRVTSRSPDSRKNYAKEIGQKYSLNVVPIDSTEEALEGADLVVSATTTSTPFVK
                     ESWLGAGVTIYSIGKNQEMESEIYKNCDKFVVDSWLHCKNKSDMQRLLKENFLSEKDL
                     YAELPELLSGKKPGRESDHERIFVRAIGLVNQDIAMANTIYQSAVDKGIGTRLPY"
     gene            3119..3352
                     /locus_tag="VFY96_04100"
     CDS             3119..3352
                     /locus_tag="VFY96_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEX5605672.1"
                     /translation="MKTVQMTLDEQLVRAVDSAARKLGTTRSGFTREALRSALKEVRV
                     RELESKHRDGYRRRPVKRGEFSDWESEQVWGDG"
     gene            3349..3588
                     /locus_tag="VFY96_04105"
     CDS             3349..3588
                     /locus_tag="VFY96_04105"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX5605673.1"
                     /translation="MKRGEVRWYVFSRPDKRRPVLILTRDSALEFLGEVTVAPITTTI
                     RDIPSEALLTKADGVPRDCAVNFDHLQTVSQGKDR"
BASE COUNT          919 a         1090 c         1074 g          785 t
ORIGIN      
        1 gagaagttcg gccgtaatcc cgcgctgctg atcatcgatg ttataaccgg tttcaccggc
       61 acgaagccga tggacgtgat ggacgccatc gacgaatttc ccaccagctg cggccaggtc
      121 gcatgggatg cattgccgaa aatcaaagag cttctccatg cctgccgtga tacgggcgta
      181 ccggtgattt attctaccag tgatccggac tttaaagccg ccttcgggaa cgcgactaag
      241 cgcggcgtcg acaaaaccga tttcgagaaa atcgccgttg agttccccga catgatcaag
      301 ccgcgagacg atgaattcat cgtccgcaag gcgcgcgcca gtgccttctt cggcacgcat
      361 ctgatcacct atctcgtgcg taagaacatc gattgcctgc tggtcgccgg caccagcacc
      421 tgcggctgtg tgcgcgggac agtgctcgac ggctactctt tcgggtatcc ggtgttcgtc
      481 gtcgaggagt gcgttttcga tcggtcgcgc acctcgcacc tggtcaacct tttcgagatg
      541 aatgccaagt acgcgagcgt gattcaactc gcggaagcct tggactatgt gaacaaactc
      601 caacgtatgg aaggaagaaa agcgatcgcg agttgattac ccttaacaag gagatcacga
      661 tggccaccga agcgccgttt cctgaggaag agtacctggc ccgctaccaa cgtgctcagg
      721 cgctgatgga tcgcgacggc gtcgatgcgt tactggtctc cgagaaaaat aattattggt
      781 actttagcgg cctgatcagc taccaactcg atcacattca gcggccacaa atcctgatct
      841 taccgaaaac tggtaaaccg ctgctgctcg tgtacggcaa tgacaaggcg aaagccaagg
      901 ctttgccgtg ggtcggcgaa gtgcgagctt atacggatgt gccatttccc gaggaaatga
      961 tcgccggctg catcaaagaa atggggcttg gccaagccaa gatcggcttc gagctcggcg
     1021 acgaccagcg tttgggcttt ccggtaaact atctcacccg tctaaccgaa gccttgcccc
     1081 aagccgagat cagagacggc accgtggcgc tcactgaaat gcgcattctc aagagtccgc
     1141 gggaaatcga gttcatgcgc aaagcctgcg agatttcggt caaagcctat gatcgctgct
     1201 tgccgcaatt gaaacccggc atgacccgcc gcgaagtcgc ggatcggctt tacatctcga
     1261 tgatcgagga aggcgcgcat ccgcgccatc cgggttttct gatgctcaac gcatcgaccc
     1321 gctatgacga tcggcgctac aaccaaggcg accgcatgat cgccgacttt ggcgcctgct
     1381 atgaaggata ctatggcgac gtcacacgca tggcgatttt cggcgcaccg accgacgagc
     1441 aacgccgcga tcacgagacc gcctgcgatg tcatcgaccg ctgttttgag tcgatgcagc
     1501 cgggcacgcc gcttgcggaa gcttcccgag tcgccaaccg cgaattgatc aaacgcggct
     1561 atgaagccgt cgagagccca aaaagaatcg gccatggtat cggcatgtcg cgcgcagagc
     1621 cgccgtcgct gaacgaagtc gaaacggaaa tctatcgtcc gggtatggtt ctcgcgctcg
     1681 agccgaaagt ccgctccgcc aagagcgcgg ttcatctgga agaagatata ctgatcaccg
     1741 acaaagggcc ggagtttttg accgccgggc gcgagatctt gagtgtgatc gattgaataa
     1801 tgaatagcct tccacccacg aagcacacga agagcacgaa gcttttacat tgaaagaaca
     1861 gtttgaactc tccgtgtcct tcgtgagctt cgtgggtttc tcactaatcg gtcgagagtg
     1921 tcgaaaatgg cagcgaatgg aattctttac ctctccaatg aggatgtgaa aaacgtcctc
     1981 gatctcggtc gcgccatcga aatcacccaa caggcgctgc gcgatcacag cgcaggccgc
     2041 gtgatctggt cgacgccgga agatctcgcg atcaaacccg agagcggctg gcaatcctgg
     2101 gttaccggct gcgctctggt cactgaacct gtcgccgggt ttcgtattcg ctcgatcaaa
     2161 gccgcgggcg gcagccgcga tgcttcgcgt gcgccgcgcg gaccccgaag aattttaatt
     2221 ctcagcgacc gcgaaggcgg cgagatccgc gccatcatgg acgaagattg gtgtcatgcg
     2281 gtgcgcaccg cggccgccgc cactgtagcc atgcgcctgc tcgcgcgcaa agactcggca
     2341 gtcatgacaa tgctcggcgc cggcgatacc gctcgcgccg ccgtgccagt catggcgcaa
     2401 gcatttcggc tgaaagaaat ccgcgtcacc tcacggtctc cggattcacg taaaaactat
     2461 gccaaagaga tcggccagaa atattcgctg aacgtcgttc ctatcgactc aacagaagag
     2521 gcgctcgaag gcgcggacct cgtggtctcg gcgacgacaa cctcaacacc cttcgtcaaa
     2581 gaaagctggc tcggcgccgg agtaacgatc tactcaatcg gcaagaatca ggagatggaa
     2641 agcgaaatct acaagaactg cgacaagttt gtcgtcgaca gctggctaca ctgtaagaac
     2701 aagtccgaca tgcagcgact gctcaaggaa aattttctga gcgaaaaaga tctctacgcc
     2761 gagctgccgg aattgctctc aggcaaaaag cccggacgcg aatcagatca cgaacgtatc
     2821 tttgttcgag ccatcggtct cgtcaatcaa gacatcgcca tggcgaacac gatttaccaa
     2881 agcgcggtgg ataaaggcat cgggacgagg ttgccgtact gatttataga ggaccgagca
     2941 gcaaagcgaa aatgcatgat tgcaagacct cacctcgtgg ctctcttgac cctcgttgct
     3001 ctctcttttc aacaactgtg cgtgtctgcg gaaatgcgtc tacgccgccg attgatttgc
     3061 ctgcagtcgt taatgtgggc cattatagct atactgatag ctacacagga gagagcgtat
     3121 gaagaccgtc cagatgacgt tggatgaaca gctggtaagg gcagtggaca gcgctgcaag
     3181 gaagctcggc accacacgat ccggttttac ccgggaagct ttacgttccg cgctaaagga
     3241 agtgcgcgtg agagaattgg agtcgaagca ccgtgacggg tacagacgca ggccggtcaa
     3301 gcgcggcgag tttagcgact gggaatcaga acaggtctgg ggtgatggat gaagcgcgga
     3361 gaggtcagat ggtatgtttt ttccagaccg gacaaaagac ggccggtgct catcctaacc
     3421 agagattcgg cgttggagtt ccttggagaa gtcaccgtcg caccgatcac cacgacgatt
     3481 cgcgatattc cgtccgaagc tttgttgacg aaggccgacg gagtgccaag agactgcgcg
     3541 gtcaattttg atcacctcca gacggtttcc caggggaagg atcgctaatc gcgaccgtga
     3601 gcccgaacaa aatgctacaa atgcgaccgt gcttgctctt tgcccttgga ttctgaattc
     3661 gcggcggtca gggaaaatcc ctcatccgtt ggccgaaagc ttcatccccc gtggcataca
     3721 aagcatcgcg ctttgattct agacctgacc ctcgcctcct cgtgctgggc ttcaacagtc
     3781 aaattgtcca gcctgactcc atgcttctgt cccgatatgc cgcattcgga aagccacccc
     3841 aactcctttc aaaaattcca cggacgaa
//
LOCUS       DASULZ010000199         3046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_633166, whole genome shotgun sequence.
ACCESSION   DASULZ010000199 DASULZ010000000
VERSION     DASULZ010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633166"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VFY96_04110"
     CDS             complement(<1..197)
                     /locus_tag="VFY96_04110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5605674.1"
                     /translation="MKLSVLMPVYNEERTLEEIVRRVCAINMPKELILVDDGSKDRSR
                     EILTRLHEENRRAGDPLNEIK"
     gene            complement(194..973)
                     /locus_tag="VFY96_04115"
     CDS             complement(194..973)
                     /locus_tag="VFY96_04115"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX5605675.1"
                     /translation="MEALSFDSRPTLRNPVLVLSFAGWSDAGASASTAIRYLIDQLLS
                     KKFASIDPEDFYDFYRQRPVVRLNESKIREIHWPSYDFYHGPGIGIEKDFVLGIGAEP
                     HLRWRTFTETMLRFARECQAEMVITLGAYLDEVLYTRPVPLTGFSTDPQLMEQLDLVP
                     SRYQGPTGIVGVLADACRAAGIPHVSLWASLPHYLSVSPNPRGTLALLLRLNQWLGLR
                     VDTTPLERAAADFQAKINDAVNGDPKLSAFVRELKKREFEQ"
     gene            complement(1056..1544)
                     /locus_tag="VFY96_04120"
     CDS             complement(1056..1544)
                     /locus_tag="VFY96_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX5605676.1"
                     /translation="MAIPERYNKGYNIGVGGAVVREHRLLLVRRASRHGHGNWQLPGG
                     FIEPDETMEQAVVREVAEETGVVSEVEAVLGLRSRYDPESGNGMYVILLLTPIRGEPI
                     ADGREVDHAAYFSLDEIRQLSPLPPVNWEIAQRVLSPDRRLLSPKPLINLNGAKFTLF
                     VG"
     gene            complement(1742..2158)
                     /locus_tag="VFY96_04125"
     CDS             complement(1742..2158)
                     /locus_tag="VFY96_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605677.1"
                     /translation="MKNGWLTFLAAGIVSLLAFAVPNNADANSRNRQKKLDESARREL
                     RKDHNELERDRRDLSRLYRNGASRDDIYRKRAEIRDDLREIAQDRRQLGRYDGYRGER
                     YRYGNYDRYDNSGWWNRGDNGWGNWGRVSDRDRWRW"
     gene            2398..>3046
                     /locus_tag="VFY96_04130"
     CDS             2398..>3046
                     /locus_tag="VFY96_04130"
                     /inference="COORDINATES: protein motif:HMM:NF024210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605678.1"
                     /translation="MAANPAYDPIDSRYYDEKDLRTEAERVFSLCADCRLCVKFCGSF
                     PKLFEAVDDYCTEGKYAEVDTKKLKQRDIDSVVDLCFQCKLCYIKCPYTPGDHEWAID
                     FPRLIARAKAQQVKKRGVPLIDRLLGNPDLVGKVGTASAGLANWANENPISRQVLHRT
                     LGIHKDKKLPPFASNSFAARFRNHRQNIRGEPVARIAFFSTCYVNYNQPEIGLDTL"
BASE COUNT          721 a          835 c          794 g          696 t
ORIGIN      
        1 atcttgatct cattgagcgg atcgccggcg cggcggtttt cttcgtggag tcgtgtcaaa
       61 atctctcggc ttcgatcctt tgaaccatca tcaaccagga tgagctcctt cggcatgtta
      121 atagcacaaa cacgtcggac gatctcctcc aacgtccgct cttcgttgta aacgggcatc
      181 aggacggaaa gcttcactgt tcaaattcgc gttttttgag ttcccggacg aaggcggaaa
      241 gtttgggatc gccatttacc gcatcattta ttttggcttg aaaatctgca gcggctcgtt
      301 ccagtggggt ggtatcgact cttaggccaa gccattgatt caagcgtaac agcagcgcca
      361 gcgttcctct gggattaggc gaaaccgata gataatgagg caatgatgcc cagagactta
      421 catgcgggat gccggccgca cggcaagcat ccgccagcac acccacaatt cccgtcgggc
      481 cctgataacg cgagggtacc aggtcaagct gttccattag ctgcggatcg gtggaaaatc
      541 cagtaagagg cactgggcgg gtatacaaaa cctcgtcgag ataggcgccc agggtaatca
      601 ccatttccgc ctggcattcc cgcgcgaatc gaagcatcgt ttcggtaaag gttcgccagc
      661 gaagatgcgg ctcggcgccg atcccgagca cgaaatcctt ttcgataccg atgccggggc
      721 cgtgatagaa atcgtatgac ggccaatgaa tttcccgtat tttactttcg ttcaggcgca
      781 ccaccggacg ttgtcgatag aaatcataaa aatcctccgg atcgatgctc gcgaatttct
      841 tgctcagcag ttgatcgatg agataacgaa tcgccgtcga ggcagaggct cccgcatcgc
      901 tccaaccagc aaacgacaac accagtacgg gattgcgcaa ggtcggtcgg ctatcaaagc
      961 ttagagcttc catagtgagt caatctagca caactaatgc gcttctctcc agggcaaaac
     1021 gatgaatcgc ggtccccgat ggtgtttagg cgcgttcagc cgacaaacag cgtgaattta
     1081 gcaccgttga gatttataag cggtttcggc gaaagcagac ggcggtcagg agaaagtact
     1141 cgctgggcaa tttcccaatt aaccggcggt aaggggctga gttgtcggat ttcatcgagg
     1201 ctaaaatagg cggcgtgatc gacttcacgt ccatccgcaa tcggctcacc gcgaataggg
     1261 gtcagaagga gaatgacgta catgccgttg ccgctctccg ggtcatagcg gcttcggagt
     1321 ccgagcaccg cttcgacttc gctaacaacg ccggtctctt ccgccacttc gcgtacgact
     1381 gcctgctcca ttgtttcatc aggctcgata aaaccgccgg gcagctgcca attgccatgg
     1441 ccatggcgcg atgcgcgccg gactaacaac aatcgatgct cgcgcacgac ggcccctcca
     1501 acaccgatgt tgtagccttt gttgtagcgc tcagggattg ccacgaaaaa ctcgatgccc
     1561 gatattgccg agagctagca taaattgcgg cgccgcatgt gaccgcccac ctgtataaca
     1621 gcgcttatag ggcgatgagc ttcgggaagc aaggagaagg aatgaagcag cgaaagaaaa
     1681 acatccgcgg aaaaatttag gggcgaccgg tggtcgcccc tagctctgaa gcaaatcgat
     1741 gctaccagcg ccagcgatct cggtcagaca cgcgtcccca gttcccccag ccattatccc
     1801 ctctgttcca ccatcccgaa ttgtcatacc ggtcgtagtt gccgtagcgg tagcgctcac
     1861 cgcgataccc gtcatagcga ccaagttgcc gacggtcctg agcgatttcc ctcagatcgt
     1921 cgcgaatctc cgctcgtttc cgatatatgt cgtcacggct ggcaccatta cggtacagac
     1981 gcgacagatc acggcggtcg cgctccaatt cgttatgatc ctttctgagc tcgcgccgtg
     2041 ctgattcatc gagtttcttc tggcggttgc gcgaattggc gtctgcatta ttgggcacgg
     2101 caaacgcgag cagcgataca atccctgccg ctaaaaaagt taaccatccg tttttcatag
     2161 gaaccctcct ttgagtcttt agactcgccg cagtggctta cgttaacggg ttcacggttg
     2221 tgttaggctc gggcaaaaaa tgagctctaa attccaggat ttgtgcggca gagatcagga
     2281 gcgtctggat cctgggaacc cgtcgaagcc aacggtcgaa cagcaaatta ctccttagcg
     2341 gctagatttt tgtaatgctc ggagatagtt tgagcctgcc tcgctcagga gtaacccgtg
     2401 gccgctaatc cagcctatga cccgattgat tcccggtatt acgatgaaaa ggatcttcgc
     2461 acagaggctg aacgcgtctt tagcctatgc gccgactgcc gcttatgtgt aaagttttgc
     2521 ggcagctttc ctaagctttt cgaggccgtt gatgactact gcacggaagg taaatacgcc
     2581 gaagtcgata ccaagaaatt gaagcagcgg gacatcgata gcgttgtcga tctctgtttt
     2641 caatgtaagc tttgctatat caaatgcccc tacactccgg gtgatcacga gtgggccatc
     2701 gattttcccc gtttgattgc cagagcgaaa gcccaacagg taaaaaaacg aggcgtgccg
     2761 ctgatcgata ggctactcgg caaccctgat ctcgtcggaa aagttgggac tgcttctgcc
     2821 gggttggcca attgggccaa tgagaatcca atcagtcgtc aggtgctgca tcgcaccttg
     2881 ggaattcaca aggacaaaaa acttccgcca tttgcgtcca actcattcgc agctcgtttc
     2941 cggaatcacc ggcaaaacat acgtggcgaa ccggttgcca gaatagcgtt cttctccacc
     3001 tgttacgtta attacaacca gccggaaatt ggtctcgata ccctta
//
LOCUS       DASULZ010000200         6593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_532180, whole genome shotgun sequence.
ACCESSION   DASULZ010000200 DASULZ010000000
VERSION     DASULZ010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6593
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532180"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..2910)
                     /gene="aceE"
                     /locus_tag="VFY96_04135"
     CDS             complement(166..2910)
                     /gene="aceE"
                     /locus_tag="VFY96_04135"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEX5605679.1"
                     /translation="MSAKIVLMKTENKSSSHNATPAENQQPNGRGNRNDRHKWVASLE
                     HILELILKNEQSGQASLLLDELTERLRQSGVKIPRTVSTPYINTIPRENEPRYPGDRE
                     IERRIKSYVRWNAMAMVVKANRLHPDIGGHISTYASSATLYEVGLNHFFRGGDGDNHA
                     DMIYFQGHASPGAYARAYVEWRINAPKLHHFRQELSPVGGVSSYPHPYLMPDFWQFPS
                     VSMGLAPIMSIYQARFVRYLQSRGLLSGAEPRVWCFAGDGEMDEPEASGALTLAAREN
                     LDNLVWVVNCNLQRLDGPVRGNGKIIQELESLFRGAGWNVVKVIWGSDWDPLLEADDN
                     GLLLKRMEEAVDGDYQKYSVEPGSYTRKHFFGKYPELLEMVNHMTDEQIHKLLRGGHD
                     PAKVYAAYQRAVENKGSPTVILAKTVKGYGLGEAGEGRNITHQQKKLNERELREFRTR
                     FDIPLPDEEVVETPFYRPPLDSRETQYLLERRRQLGGFVPERRVRAKPLNVPDENSYA
                     ELLRGSAGHPMSTTMATVRLLTKLLHHDEIGKRIVPIIPDEARTFGMDSLFRQIGIYS
                     PKGQLYEPVDRETLLYYNEAKDGQILEEGITEAGSMSSFIAAGTAYANLGLNMIPFYF
                     YYSMFGFQRIGDLIWAAADNKAKGFLLGATAGRTTLNGEGLQHQDGHSHLLASTVPTL
                     LTYDAAFAYEIAVIVKDGLRRLYSEGEEYFYYLTLYNESYEMPPMPGGTEEGILKGLY
                     KLKPASKGKKFKAHLFGSGPLLREALRAQEILAEQFDVSADVWSATSYKLLRGDALRT
                     RRWNMLHPTATPKKSYIESVLQDEEGVFIAVSDYMKMVADQITPWVPGGLLTLGTDGF
                     GRSDTRENLRRFFEVDAEIATVATLYALYQKGLLAGHAVEQAIKKLGVDPEKPFPLYL
                     "
     gene            2940..3659
                     /locus_tag="VFY96_04140"
     CDS             2940..3659
                     /locus_tag="VFY96_04140"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEX5605680.1"
                     /translation="MKYLDLAFANPAHNLACDEALLQLMETGQVKDEILRVWEPAQYF
                     VVLGHANAIRAEVNLSACEEDRVTVLRRMSGGGAVLQGPGCFNYSLVLNGAVHRFRTV
                     REGFRHVLERHRNIIQALTGFEVALEGISDLAIGARKFSGNAQYRKSCAVLIHGTFLL
                     DFDVSLIARYLTLPVKQPAYRQNRSHLEFLTTVPVRSAELRERLRISWSADGALDQIP
                     ADRIDGLVQERYGRNEWSEKF"
     gene            complement(4606..4785)
                     /locus_tag="VFY96_04145"
     CDS             complement(4606..4785)
                     /locus_tag="VFY96_04145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5605681.1"
                     /translation="MQKRVDMPLLTPREAAKLLNLPTNTVRKMIERKELLAYKIGRRW
                     RIPQPEVTKLKPPPH"
     gene            complement(5375..5575)
                     /locus_tag="VFY96_04150"
     CDS             complement(5375..5575)
                     /locus_tag="VFY96_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605682.1"
                     /translation="MEIENELGFDGNRDDSVRLRAHVSAEIVNQISTPAGKSLAGCDP
                     SIQCAVGAELLNCPDYGADAML"
BASE COUNT         1531 a         1757 c         1798 g         1507 t
ORIGIN      
        1 gaatcgagcc tacggccttc tgagtttcca attccatgaa gggttggtct tttttgactt
       61 cttggccttc ggcaaccaga atgctgacga ctgtgccgga ctctacgcct tcggcgagct
      121 gcggaagttt gacgtccatg ctgaagattt gcggtgatcg agtatttaaa ggtaaagcgg
      181 gaatggtttt tcgggatcga caccgagttt tttgatcgct tgctcaaccg catgcccggc
      241 cagtaagcct ttctgataga gcgcatagag ggttgccacc gtggcgatct ctgcatcgac
      301 ctcaaaaaag cggcgcaggt tctcgcgcgt atcgctgcgg ccgaacccgt ccgtgcccaa
      361 cgtcaacaat ccacccggca cccacggcgt gatctggtcg gcgaccatct tcatatagtc
      421 cgatacggca atgaacacgc cctcttcgtc ctgcagcacc gattcgatgt acgatttctt
      481 tggtgtcgcc gtcgggtgca gcatgttcca gcggcgagtg cgcagggcgt caccgcgcag
      541 gagcttataa ctggtcgcgc tccagacatc ggcggaaaca tcgaactgct cagccaagat
      601 ctcctgcgct cgcagcgctt ctctcagcag cggtccgctg ccgaacagat gcgctttgaa
      661 cttcttacct ttactcgcgg gtttgagctt gtacaaacct ttgagaattc cttcttccgt
      721 accccccggc atcggcggca tttcgtaact ctcgttatac agcgtcaaat agtagaagta
      781 ttcttcaccc tcgctataga gccggcgcag accgtcttta acgatgactg cgatctcgta
      841 ggcgaaagcc gcatcgtagg tcagtaaagt tggaaccgtg ctcgccaaca aatggctatg
      901 gccgtcctga tgctgcagcc cctctccatt aagagtcgtg cgtcccgcag tggcgccgag
      961 gagaaaaccc ttggctttgt tgtccgcggc cgcccagatg agatcgccga tgcgctgaaa
     1021 gccaaacatc gaataataga aatagaaggg aatcatattg agtcccagat tggcgtaagc
     1081 ggttccggcg gcaataaacg atgacatcga accggcttcg gtaattccct cttccagaat
     1141 ctggccgtcc ttggcttcgt tgtaatagag cagagtctca cgatcgaccg gttcgtagag
     1201 ctggcctttg ggagaataga tgccgatctg ccgaaatagc gagtccatgc cgaaggtgcg
     1261 cgcctcgtcc gggatgatcg gcacgatgcg cttgccgatc tcgtcgtggt gcaacagttt
     1321 ggtgaggagc cgcaccgtcg ccatggtggt cgacattggg tgaccggcgg agccccttaa
     1381 aagttccgcg tagctatttt catccggcac gttgagcggc ttggcccgca ctcttcgttc
     1441 cggcacaaaa ccgccgagct ggcgccggcg ttccaacaga tactgcgtct cgcggctatc
     1501 gagcggcggc cgatagaacg gggtctcgac gacttcttcg tcgggcaatg gaatgtcgaa
     1561 gcgagtgcga aactcgcgca gctcacgttc gttcagcttc ttctgctgat gcgtgatatt
     1621 gcgcccttca cctgcttcac ccaagccgta gcctttgaca gtcttggcca gaatcactgt
     1681 cggcgaacct ttgttttcga cggcacgttg gtaagccgca tacaccttcg cgggatcgtg
     1741 accgccccga agcagtttat ggatctgctc gtcggtcatg tggttgacca tttccaaaag
     1801 ctctgggtat ttcccaaaga aatgcttccg ggtatagctt ccaggctcga ccgagtactt
     1861 ttgataatct ccgtcgaccg cttcttccat gcgcttcagc aataatccgt tgtcatcggc
     1921 ttccagcagc ggatcccagt ctgatcccca gatcaccttg acgacgttcc agccggcacc
     1981 gcgaaaaagc gactccaatt cttggataat tttgccattg ccgcgtacgg gaccatctaa
     2041 acgctgaagg ttgcagttca cgacccagac gagattgtcg aggttttccc gcgcggcgag
     2101 tgtcagggct ccagaagcct ccggctcatc catttctccg tcgccggcga aacaccagac
     2161 gcgtggttcg gcgccgctca ataagccgcg actttgcaga tatcggacga agcgagcctg
     2221 ataaatggac atgatcggcg cgagtcccat ggaaaccgat gggaattgcc aaaaatcggg
     2281 catcaaatac gggtgcggat aagatgagac gccaccgacc ggcgagagct cctggcgaaa
     2341 atggtgcagt ttgggcgcgt tgatgcgcca ttcgacataa gcgcgagcat aggcgccggg
     2401 tgaggcatgc ccttgaaagt aaatcatgtc cgcgtgatta tcgccgtcac caccgcggaa
     2461 aaaatggttc aaaccgactt cataaagcgt ggccgacgaa gcgtaggtgg aaatatggcc
     2521 gccaatgtcg ggatgaagac gattcgcttt gacgaccatc gccatggcgt tccaacggac
     2581 gtagctcttg atccgccgct caatttctcg gtcaccggga tagcggggtt cgttctcccg
     2641 cggaatcgtg ttgatatagg gggtgctaac cgtgcgagga attttcacgc ccgactggcg
     2701 caggcgttcg gtgagttcat ctagcagtag gctcgcttgc ccagattgct cgttcttgag
     2761 aattagttcg agaatatgct caagtgaagc aacccattta tgccgatcgt ttctgttgcc
     2821 cctgccgttg ggctgctgat tctcggccgg cgtcgcatta tgagaactgc ttttgttttc
     2881 cgttttcatc aagacaattt tagcagacaa caaaattcgg ataaaatcaa ccaaacaaat
     2941 tgaaatacct tgacctcgcc ttcgctaacc cggcgcacaa tcttgcctgt gacgaagcgc
     3001 tgctgcaact gatggaaaca ggacaggtta aagatgaaat attgcgcgtc tgggaaccgg
     3061 cgcagtactt tgtcgtgctc ggccatgcca acgcaatccg cgcggaagtc aatttgtccg
     3121 cctgtgagga agatcgcgtg accgtcctgc ggcggatgag cgggggcggt gccgtgttac
     3181 agggacccgg gtgcttcaat tactcgttgg ttttaaacgg cgcagtacat cgcttcagaa
     3241 cagtcagaga aggctttcgc cacgtattgg agcgccacag gaatatcatc caggcattga
     3301 ccggcttcga agtcgctctc gaaggtatca gcgatttggc gatcggcgca cgcaagttct
     3361 ccggcaacgc gcagtaccgc aaatcctgtg cggtactaat tcacggcacg tttctcctcg
     3421 attttgatgt gtctttaatc gctcggtatc tgacgcttcc tgtcaaacag cccgcctatc
     3481 gacagaatcg ctcgcacctg gagtttctca cgactgttcc ggtgcgctcg gcagagttgc
     3541 gcgaacgcct aagaataagt tggagtgcgg atggagctct agatcaaatt ccagctgatc
     3601 gtattgatgg actcgtgcaa gagcgctatg gaaggaacga atggtcggaa aaattttaac
     3661 gcgaaggtca atgagaacta agcgaggttg atgaaatgaa ttctggaccc tttgttcggc
     3721 gattgtttgc ggcatgagtc gtcaaggacc gtcagacgtt cagcagaaat gacgcctctt
     3781 aagcttgcgg ttactctatg tagtgttttg ttgttcggct gcgccgcgca cacctggagc
     3841 aggccgaaca ccaccgagca agaaatgcgc cgtgaccttg aacaatgtga gcgcgaccgc
     3901 aacgcaatcg caggcgagtc gtttttcctc gagcgctgca tgttctcaaa gggctatact
     3961 gaaaaatgaa agcgcacaag atccggtggc gaatggtggg agtgcgagct ataacgcgac
     4021 ggatgtgggc cgtatggcta atctttctcc gaggtgagct ggcctacggc taactcccaa
     4081 tgcttcgggc aaagatagat gaatctgcag cggctttcat caagaaatac gtttttacga
     4141 ccgtggtcat ttagtaagct catctccaca ccatcaagaa tgcccatttc catacagtcc
     4201 gcccaatcgc accggttcat gggtgattat tctcaccgat ggaagtgcca gatgacaagc
     4261 ttttttccga ccaatggaaa aattactgaa aaagtctctg ggaatatcaa caagcgccaa
     4321 atctccccgg ccgagccatc cctcggcctt aagcgccggc acaaagtgac aaccttaacg
     4381 agagatcctc gcaggcgggc aatcggataa tcacaatggc gcgaacggca acagaggggt
     4441 aagctcgccc gatgggtcga aggtgaacct atgttcccgg catccagggc cggccgatta
     4501 tgttggcgcc aaagacgaac tgcccttcgt agtggtgctc gccttcaaag cagaaataca
     4561 ataccaatgg gcggaaattc gtgaacgatg caactgagcc caagctcagt gagggggtgg
     4621 tttcagctta gtgacttcgg gctgagggat acgccagcgt ctgccgattt tgtaggccag
     4681 caattctttt cgctcgatca tttttcgtac tgtgttggtt ggcagattca gcaatttagc
     4741 ggcctcgcgt ggagtcagca acggcatgtc aactcgtttc tgcatagccc gccctcctcc
     4801 attgcccctt gtttactcgg acatgagaga gttggcaaca attgaaagga cattataagc
     4861 aagcgaaaat ctcggccggc cacggcccca ttgaatcatc gtcgcgtcat ctaagcgtcg
     4921 tccaacgcgg ctgctccgct cgatggccac gcattgcact tctttttaac ggctcgacgt
     4981 tatctatttt ttcagccacg aattgtttcc cacagcgcgg attcttgccc actcttagtg
     5041 attacattcc ccgtcgatga cgccgattgt ctgattcaga accgaattat gagtggtgta
     5101 attttccgct gcacgaatgc gatcgtccga tctgggccag ttgaaaaaaa tagagagctg
     5161 gtgaaagtgc taaccaaaat ggacgtctga aaggtaatag cagtggcggt ggtaggagtc
     5221 gaacctacga cgccgcggat atgacccgcg aggagtggga tgaaagctac tgaaaacaca
     5281 gtagctttat ttctgggggt gttctcgggg ggctattaga gtttgaaaac ccgccaaaac
     5341 atggccaagg ggcaccaaga ggaatcaaaa ccgcctaaag catggcgtcc gcgccgtaat
     5401 cagggcagtt aagaagctcg gcgccaaccg cgcactggat agacggatcg caaccggcaa
     5461 ggctcttgcc cgctggcgtc gagatttgat tgacgatctc ggcggagacg tgagcacgca
     5521 gccggacgct atcatctcgg ttgccgtcaa aaccaagctc attctcgatt tccatcgctg
     5581 tttggctact tcatccatcg cttattgata agcggaagag ggcgcttcta ccggccgtca
     5641 gagagcgcca acccctgggc gatgccttgc cgcgctattt tgccatgctc ggccttgagc
     5701 gcctaaacaa ggtgaagagc ttaaacgagc tgctcaccga gcccgaagga gcgagcacgg
     5761 caacgacaag gcggattgat ggaagcggcc gcgttaacgc agtcagacag ccgaactgag
     5821 gccaaccttc aagcagagcc ggtagcgtgc ggcaatctag tcgttacgac tagcagcggc
     5881 cattctcagc gctgcgctaa cccgttctgt aagtcgacat tgagctgttg aaccatgacc
     5941 gatggcggag gacgcaacgg cgcttctgct ctgatgcctg caaatacgat tatcacgcgc
     6001 tcaggcgggt caagacgctg ctgaatcgca gtgccgtaaa cgattttctc cggctgttag
     6061 accaaatgcg atgaacgatt tagtcgacta gcggtccaag tcagaaacaa cggaaaaaat
     6121 aacgagtgct acggccatag aaaaggcgct gacgccaaac ccgatgagaa gagaaatcgc
     6181 gagaatcaca aattcagcgt aggttttccg caatgcgggt tcaagatact gccgtcggcg
     6241 ctgcctaata gagccggcaa tatcgagttt caccgggtgt tggatcagtt gtatgaatga
     6301 atccggcccg gaccaacccc tcattatcac gtttttacgc gcgttggatg tgcgttgtcg
     6361 aaatgacgtc ccagtctttt taggaatcgc gctttgcaat gcaggcagcg gtaaggccaa
     6421 aggccaaaga tgctgatggg cttttcaaaa aaatatcgat gagaccgccg catgttcatg
     6481 gttgaacagt acggacatga ttccttagtc agcattctga gtcgaacgag caatttggac
     6541 gagcttagct tctctgaagc ctgggcaagt gaatcagaca gcaattggtg agc
//
LOCUS       DASULZ010000201         3588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_126098, whole genome shotgun sequence.
ACCESSION   DASULZ010000201 DASULZ010000000
VERSION     DASULZ010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126098"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1419)
                     /locus_tag="VFY96_04155"
     CDS             complement(25..1419)
                     /locus_tag="VFY96_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5605683.1"
                     /translation="MLLIVDDEPGVRQSLQLVFNKTYRTIEAASAEDAIQKTTEENPD
                     LILLDIMMPGTDGLAVLKQIKSANGDCQVIMLTGLNTARTAFAAKATGAFDYVTKPFD
                     IEELRLRVEHALEKVQLSRELERLKEEVGRKYGIDYVIGKSKQIIEVFKSVSMVAAKK
                     STVLITGESGTGKELIARAIHYNSDRRSKPFVVVNCAALPDTLIESELFGYERGAFTN
                     ASQKKVGRFELAHGGTLFLDEIGELNLGTQAKFLRAIEQETFTRLGGTDEIKVDVRII
                     AASNRDLEQMAKNAEFRPDLFYRLNVVALFLPPLRERRDDIPLLLDHFLRLKAQEHSI
                     AAKSLSPEVVDFFTAYPWPGNIRELENLIERLTILTPHETIMLRDLPIGMRSTDQTAT
                     LKEDVLSGSRPLSDAVDEFERELIVKALQRTGFNQTKAASLLGTSRRILKYRIEKLKI
                     TDPNEADSEVKLST"
     gene            complement(1433..3172)
                     /locus_tag="VFY96_04160"
     CDS             complement(1433..3172)
                     /locus_tag="VFY96_04160"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX5605684.1"
                     /translation="MLILGISPRYGMEARSLTRFAAQLPFAVLFLVDSKSATRIVPSE
                     AQVGFLTKPFNPYELHEKVGQLLARQAAVPKTTTTPQVLQDIRYLDYPYLSRSSASIV
                     HRFASARLPLLITGELGCGQDRVVSAVCHLERTPGFRLSIDAGEADSEYLTQKALQLS
                     FHEGFVAAPITIVIHDLDRSPSSAQSTLFNFTQDIEQKLPNVRYLSTATNDLLEKVFR
                     GEFLESLYYRLATLTLKLSPLRERQEDIVVLAGWFARIYGQVLGIGEPVISPEANSRL
                     RNYLWFGNVKELESVIARTLALRRKLTIAADDLVFEFGETTVSSEELSDYSEVTRDRS
                     AMELTSPHSGFGPYSVANEKNGHGRSVDLNVLIHELAHEFKNPMVTIKTFAQLLGDRY
                     QDENFRTRFQEVVGGDIERMDELLEMMIEFADFSQPQPGDLSLDDKLRAIVVELQAEC
                     AKRQTQFAWQENGATHVIRADEAQLAYILRNVLLAMLCETKIGTEIRLQLTSEGVLTI
                     SHLRDEPRMASIGRDIENASSRPIESILPLRVLLAKYLLEKNGGRFTREPCDDDRETL
                     RLEFPIAEHGQKN"
     gene            complement(3291..>3588)
                     /locus_tag="VFY96_04165"
     CDS             complement(3291..>3588)
                     /locus_tag="VFY96_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5605685.1"
                     /translation="SGADPVGEFVQVEVRDTGIGIPEDNLQHIFDPFFTSKDEGSGLG
                     LAVSHQIVQEHGGFVTVESTVGKGTAFFVHVPVAKPVRPAANGRAQLNEANLSH"
BASE COUNT          813 a          999 c          925 g          851 t
ORIGIN      
        1 acttcaggga acaaatttgt ccaattaggt cgataatttc acttccgaat cggcctcgtt
       61 tggatctgta atcttcagct tttcgatgcg gtacttgaga atgcgtcggc tggtacccag
      121 tagtgatgcg gccttggtct gattgaaacc ggtgcgttgg agcgctttaa cgatcagctc
      181 tcgctcgaac tcatccacgg catcgctgag cggacgtgaa ccgctcaaca catcctcttt
      241 gagagtcgcc gtctgatctg tagatctcat cccgatcggt aggtctcgca gcatgatggt
      301 ttcgtgtggc gtcaatatgg ttagccgctc gatcaggttt tccagctcgc gaatattgcc
      361 gggccacggg tatgcggtaa aaaaatcgac cacctcggga gacaagctct tagcggcgat
      421 cgaatgctct tgagctttca accgcaagaa gtggtcgagt aacagcggaa tatcgtcgcg
      481 ccgctctctc agcggtggca gaaagagcgc cacaacgtta aggcgataaa acaagtcggg
      541 acgaaactcc gcatttttag ccatctgctc gaggtcgcga ttacttgcgg caatgatgcg
      601 cacatcgacc ttgatttcat ccgtcccccc cagacgcgta aaggtttctt gctcaatggc
      661 gcgcaaaaat ttcgcttgag tcccgaggtt gagctcaccg atttcgtcga gaaacagcgt
      721 gccgccatgc gccagctcga atcggccaac tttcttttgg gaagcgttgg tgaatgcgcc
      781 acgctcatag ccgaagagct cgctttcgat cagagtgtca ggcaatgccg cacaattcac
      841 cacgacaaat ggttttgagc gccgatcgct gttgtaatga attgcccggg cgatgagttc
      901 tttgcccgtg ccgctctctc cagtgatcaa aacggtactc ttcttggccg ccaccatgct
      961 gacagacttg aaaacctcga taatctgttt cgatttgccg atgacgtaat cgataccgta
     1021 ctttcgaccg acttcttcct tcaagcgttc tagttcccgc gacaactgca ctttctcgag
     1081 cgcgtgttcg accctcaatc gcagctcttc gatatcaaaa ggcttggtga cataatcaaa
     1141 ggcgcccgtc gcttttgcag caaaggccgt gcgcgcggta ttaagtccgg tcagcataat
     1201 cacctggcag tcgccgttcg cggatttaat ttgtttcagc accgcgaggc cgtcggtccc
     1261 aggcatcata atatccaaca gaataagatc ggggttttct tccgttgttt tttggatcgc
     1321 gtcttcggcg gatgccgcct cgatcgttcg ataagtttta ttgaaaacga gctggagcga
     1381 ctggcgcact cccggttcgt catcgactat caataacaac ggttggctct tatcagtttt
     1441 tctgtccgtg ctctgcaatc gggaactcca atctcaatgt ttctctgtcg tcgtcgcacg
     1501 gctcgcgcgt aaaacggccg ccgttttttt ccaaaagata cttggcaagc aatacccgga
     1561 gcgggagaat actctcgatc ggtcgggaag aggcattctc gatgtctcgg ccgatcgaag
     1621 ccatccgcgg ttcgtcgcgt aaatggctga tcgttaacac gccttcggac gtgagttgca
     1681 gccggatttc ggtaccgatt ttagtctcgc acaacatcgc caacaagacg ttccggagaa
     1741 tgtaggcaag ctgcgcttcg tccgcccgga tcacgtgagt cgcgccgttt tcctgccaag
     1801 caaactgcgt ttggcgcttc gcgcactcgg cttgaagctc gaccacgatc gcgcgcaatt
     1861 tgtcgtcgag cgagaggtct ccgggttgcg gctgactgaa atccgcaaac tcgatcatca
     1921 tctccaacaa ttcgtccatc cgctcgatgt cgccgccgac gacttcttgg aagcgcgtgc
     1981 gaaagttttc gtcctggtag cgatcgccga gcaactgcgc gaaagtcttg atcgtgacca
     2041 ttgggttttt gaattcatgc gcgagctcat gaatcagcac attcaaatcg acggaacggc
     2101 catggccgtt cttctcgttc gcaaccgaat agggcccaaa gcccgagtgc ggcgatgtga
     2161 gttccatggc tgaacgatcg cgcgtgactt cggagtagtc cgataattct tccgagctta
     2221 cagtcgtttc gccaaactca aagactagat catccgcagc gatcgtaagc ttgcgccgca
     2281 gcgcaagcgt tcgagcaatg acactttcca actctttgac gtttccgaac cacaggtaat
     2341 tacgcaatcg gctgttcgcc tccggcgaga ttaccggctc accgatacct agaacttgcc
     2401 cataaatacg agcgaaccat ccggccaata ccacaatgtc ctcttggcgc tcgcgtaatg
     2461 gcgacagctt taacgtcaag gtggcaagcc tatagtataa cgattcgaga aattcgccgc
     2521 gaaatacttt ttcgagcaga tcattcgtcg ccgtcgagag atagcgcacg ttcggcagtt
     2581 tctgttcgat gtcctgggta aaattaaaca gcgtcgactg tgccgatgac ggactcctat
     2641 ccaagtcgtg aataacgatc gttatgggcg ccgctacaaa gccctcatga aacgagagct
     2701 gcaaagcttt ttgtgtgaga tactcagaat ccgcttcgcc ggcatcgatc gacagccgga
     2761 aaccaggcgt acgttccaga tggcacactg ctgagacaac acggtcctgc ccgcagccga
     2821 gttcgccggt gatcagtagc ggcaaccgcg ccgaagcaaa tcgatgaacg atactggccg
     2881 aactgcgact caagtacgga taatcaaggt agcggatatc ctggagaacc tgtggagttg
     2941 tagtggtttt tggcacagcc gcttggcgcg ccagaagttg cccgactttc tcgtgtagtt
     3001 catacggatt gaatggcttg gtgagaaaac cgacttgagc ttcggacggg acgatccgcg
     3061 tggccgactt tgaatctact aaaaaaagca ccgcaaacgg gagctgcgca gcaaagcgcg
     3121 tgagacttcg cgcctccatt ccataccggg gcgagattcc aaggatcaac aagtcaactt
     3181 cggcttgcgc atcggtaaaa gaaaacccct gagtgcccag cgcgcgtttc gaaacggcga
     3241 actcgcgacc gagaatcagc gccagcgtct ctcgtacgac ttccgcttca tcaatgactc
     3301 agattcgctt cattcagctg agcgcggcca ttcgcggcgg ggcgcaccgg ctttgcaacc
     3361 ggcacgtgaa cgaaaaacgc ggttcctttt ccaaccgtac tctcaacggt gacgaaaccg
     3421 ccgtgttctt gaacgatttg gtgtgacacc gccagaccca atccactgcc ttcatcttta
     3481 ctcgtgaaga acgggtcgaa aatgtgctgc agattgtctt caggaatgcc tatgcccgtg
     3541 tcgcgaactt caacttggac aaactcgccg acgggatctg ctccactc
//
LOCUS       DASULZ010000202         2995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1038613, whole genome shotgun sequence.
ACCESSION   DASULZ010000202 DASULZ010000000
VERSION     DASULZ010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038613"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VFY96_04170"
     CDS             complement(<1..736)
                     /locus_tag="VFY96_04170"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="PtrB; oligopeptidase that cleaves peptide bonds
                     following arginine and lysine residues; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HEX5605686.1"
                     /translation="MVKKIPTKLEKHGHVRIDNYYWLRERENPEVIRYLNEENEYSAK
                     EMDHAREFEERVFQEIKGRFKQTDMSVPYKRDDYFYYTRYEEQKEYPIYARKRGSLAQ
                     AEEIILNANILAQGHEFFSVGGWAVSSGQDLLAYAVDIEGRRIHTAYLKNLFTGELLP
                     DVLPNVTENLAWANDNRTLFYAKQNEVTLRQYQIYRHEVGTDPAKDQLVFQEDDETFV
                     TYIFKTKSKKFLMIVSAQTVSQEYRYL"
     gene            complement(826..1659)
                     /locus_tag="VFY96_04175"
     CDS             complement(826..1659)
                     /locus_tag="VFY96_04175"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX5605687.1"
                     /translation="MSRSDGRLFVALAACVIFSSILPEGACAQSKGRDTIDDLLKSIG
                     LSKPAFTRAPDFSLSDAVGGIASISGNRGSLMLVNFWATWCGPCRDEMPSMEQLSRSF
                     GGQGFSVLAVNQRENAGQVLRFMKTHGLNFRAPLDTDGRVAAAYRVYGIPATYLIDGA
                     GQALGMKAGPKDWAARGVIEVFRKLVSERGSTVASSSMDFEPNAPLTKMLRPRNNGLL
                     VHAQQDPQSEILAKLDRSDDLVALGKVSGASGYWYMVRTKNGVAGCVRETEVEPTSDQ
                     K"
     gene            1849..>2995
                     /locus_tag="VFY96_04180"
     CDS             1849..>2995
                     /locus_tag="VFY96_04180"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX5605688.1"
                     /translation="MRFARRSVLFLFTVVGVFCAGPQTVVAQARTKAIVLAWDGTVPS
                     FVREMLRAGKLPHLAKLIEGGAFADDVRPGFPSKTAPGFASLMTGAPPSITGISGNRV
                     PRAPRNEFTILDSLAGFSEAPLQAEPIWSVAMHAGKKVVISHIPTFAGERSEQAVRFS
                     GYDLSGGRDGIVTKRAIQNAPADAGTESPASDAPPVEITFSVGETRFIGLLIDDPSDS
                     QIGYDTLLLATARNPHEIKAKLKPAPASAAGELFWSTPIAVAVAGNQEAKTYFRLFDL
                     KSDGSDFFLYYTRPARDLPIPQTDVAPGSTVQTFIGNGASILYNQGAFGRTIPNGGSG
                     GAEARYLETVTMAQHQLMETNRWAIEQLSWDVFLAYTPFPDEAEHVWR"
BASE COUNT          639 a          854 c          802 g          700 t
ORIGIN      
        1 ctaagtagcg gtattcttgg ctcacggtct gagctgaaac aatcatcaag aatttcttcg
       61 atttggtttt gaagatgtag gtgacgaacg tttcgtcgtc ttcttggaaa accagctgat
      121 cctttgccgg atcggttccc acctcgtgcc gatagatctg atactgtcgc agcgtgactt
      181 cgttctgctt ggcgtagaaa agggttcggt tgtcgtttgc ccaggcgaga ttttcggtca
      241 cgttcggcag gacatcgggt aaaagctcgc ccgtaaacaa gttcttgaga tacgcagtgt
      301 gaatacgccg gccctcgata tccacggcat aagccagtaa atcctgcccg gagctgaccg
      361 cccagccacc gacggaaaag aactcgtgac cttgggcgag aatgttggcg ttgaggatga
      421 tttcttccgc ttgcgcgagg gaaccgcgtt ttcgcgcgta gattggatac tccttttgct
      481 cctcgtagcg tgtatagtaa aaataatcat cgcgcttgta aggcaccgac atgtcggttt
      541 gtttgaatcg ccctttgatt tcctgaaaga ctctttcctc gaactcgcgg gcgtggtcca
      601 tctctttggc ggagtattca ttctcctcgt tcaggtatcg gatcacctcc ggattttccc
      661 gctcgcgcag ccaataatag ttatcgatcc gcacgtgccc gtgcttctcc agtttggtag
      721 gaattttctt gaccattgat ggctgtcttt gtacgagctc agggaatgac tagcgggagc
      781 ggccctccct tacgcgcagt aagagacgca tagaaaagat tttgatcact tttgatcact
      841 cgttggttcc acctccgttt cccgaacgca tcctgccaca ccatttttcg ttctaaccat
      901 gtaccaatac ccgcttgcgc cggaaacctt gccgagcgca accaagtcat cgctgcggtc
      961 aagcttggca agaatttctg attgcgggtc ctgctgagcg tgcactagca gtccgttgtt
     1021 cctgggccgc agcatcttgg ttagcggcgc gttcggttcg aaatccatcg aactgctcgc
     1081 aactgtactg ccccgctcgg aaaccaattt ccggaatact tcgatgactc ctcgagcggc
     1141 ccagtctttc ggccccgctt tcatgccgag cgcctggccc gccccatcga tcagataagt
     1201 tgcgggaata ccgtagactc ggtaagcagc cgccacgcgc ccgtcggtat ccagtggcgc
     1261 gcgaaagttg agtccgtgcg tcttcatgaa ccgtaacacc tggccggcgt tttcccgttg
     1321 attcacggcg agaacactga agccttggcc gccgaaggag cgactcagtt gctccatgga
     1381 gggcatttcg tcgcggcacg gcccacacca cgtcgcccaa aaattgacca gcatcaggct
     1441 accccgattg ccactgatgc ttgcgatgcc gcccacagca tcactcaagc tgaaatctgg
     1501 cgcgcgcgta aacgccggct tactcaaacc gatcgatttc aacagatcgt cgatcgtatc
     1561 cctgcccttc gactgtgcac atgcgccctc gggcaggatc gagctaaaaa tcacgcaggc
     1621 cgcaagggcg acgaaaagtc tcccatctga tcggctcatg aagcatcctc caaattcggc
     1681 caaccccacg ctcataccga atccgcgctg atctgtctaa cgcgagaacg aaaatcccag
     1741 tgaattgacc gtcttaacct catcggctac tctcagcctc gccttagcga taaagcaggc
     1801 gagggaaggt ttgatgaaca aaatagattg cctgggaatt ggtcctaaat gagattcgcc
     1861 cgtcgatctg ttctttttct gttcaccgtc gtaggggtat tctgcgccgg gccgcagacg
     1921 gttgttgctc aagcgcgcac caaagcgatt gtgcttgcct gggacggcac ggttccgagc
     1981 ttcgttcgcg agatgctgcg cgctggcaag cttccccatt tggcaaagct gatcgagggg
     2041 ggcgcgtttg cggacgatgt cagacccggc tttccttcca aaactgcgcc tggctttgca
     2101 tcgctgatga ccggcgcacc accaagcatc accgggatca gcggcaatcg tgtgccgaga
     2161 gcgccgcgca acgagtttac catcctggac agcttggctg gattttctga agcaccgctt
     2221 caggcggagc ctatctggtc ggtggcaatg catgccggca aaaaagtagt gatatctcac
     2281 atacccacgt ttgccggcga gcggtccgaa caagccgtgc gattttccgg atatgatcta
     2341 agcggggggc gcgatggcat tgtcaccaaa cgtgccattc aaaacgcacc ggcagacgcc
     2401 gggactgagt cgccggcaag tgacgcgcca ccagtggaaa tcacgttttc cgtcggtgaa
     2461 acacgcttca tcgggttatt gatcgatgat ccatcggata gtcaaattgg ctatgacacg
     2521 ttactgctcg cgacggcccg caatcctcac gagataaaag cgaaactgaa gccagctcca
     2581 gcaagcgctg ctggagaact gttttggagc actccgatcg cggttgcggt tgccggcaat
     2641 caggaagcaa agacttattt tcgactgttc gatctgaagt cggatgggag tgattttttt
     2701 ctttactaca cgcggccggc gcgcgatctg cccatcccgc aaacggacgt ggctccgggc
     2761 agtaccgtgc agactttcat cggcaatggc gcgagcatcc tttacaacca aggcgccttc
     2821 gggagaacca ttccgaacgg cggctcgggc ggtgccgaag cacgctacct tgaaactgtg
     2881 acaatggcgc aacatcagtt gatggaaacc aaccggtggg caattgagca gctttcctgg
     2941 gatgtatttc tcgcctatac gccttttccc gatgaggcgg agcatgtctg gcgcg
//
LOCUS       DASULZ010000203         2619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_734862, whole genome shotgun sequence.
ACCESSION   DASULZ010000203 DASULZ010000000
VERSION     DASULZ010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734862"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="VFY96_04185"
     CDS             <1..846
                     /locus_tag="VFY96_04185"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX5605689.1"
                     /translation="AAARQAQAELDQLVSGSRRHELDQVKAEWSANKAQAELLRKSLK
                     RTEDLSRRELIPRQEYEDALGKTEEAEQRARVARERYETLLAGTRPEDLERVKQRLAE
                     AEARLRQLRSGFRKEEIAQAKSAVEAAQAKVQWIRTQLDETVIKAPADALVEKLDIEA
                     GDLVSAGKPVALLVRTGSLWVRTYLPQAQLRFARPGTKANVRVDVYPGTDFDGVIRRI
                     HLENDSTPSKLLSREERALQVVQTEVMVADPDNMLRPGMNADVIIAKNDARTTAQAQP
                     ELRAD"
     gene            complement(864..2084)
                     /locus_tag="VFY96_04190"
     CDS             complement(864..2084)
                     /locus_tag="VFY96_04190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5605690.1"
                     /translation="MIAITSLFGMEKLPPAKARALSIVYASSIALMMGVNFLQPALPA
                     LTQPFNISDAQLSWIMTLFTAPAIVLSPIFGAVADLYGRRLLLGFGLITFGLFGTAMA
                     FAPSFGWLLALRTLQGVGFSAVIPLTIVLIGDLLEGDNEIGGQGLKVFLDRIGYFIFP
                     PLGGVLAAIAWFWPFVCYALAIPLGLIALIWMPETKGKTGDKATAYVADILRLTRHPR
                     LVIAFSAGFLRFFLDYGFLTYFPLFLVRTQNVSTATAGLLYIFFSVGAMITSSQAGRI
                     AAGHDKANILFLSFVVSGAAVSAVPFIHGPWLVGCALFFYGLANGVISPMQKSLLTQN
                     APAELRGGIVSFDRLIQQVSKTTSTSIVGLLLVSAELPTIFWILGILSFISVGLMACL
                     LPRGQPMVARQPAS"
     gene            2219..>2619
                     /locus_tag="VFY96_04195"
     CDS             2219..>2619
                     /locus_tag="VFY96_04195"
                     /inference="COORDINATES: protein motif:HMM:NF045191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605691.1"
                     /translation="MPLDMRTWIDQLEKAGLLARIAKPVDPRTQMGALLWQARDRALL
                     FENLPGYPGWRCLGQAPGDVSLAPAAFGTERDEMIPEFVRRTEKLGTTRLVETGPVQQ
                     KVLIGDEVDITKMPIHQAGIRDGGPFIGSGL"
BASE COUNT          644 a          737 c          811 g          427 t
ORIGIN      
        1 gccgcggcca ggcaggctca agccgagttg gatcaactcg tctccggttc gcgcaggcac
       61 gaactcgatc aggtgaaagc ggagtggagc gcgaacaaag ctcaggcgga gctcttgcgt
      121 aagtctctga aacgcaccga agatctgagc cggcgagagt tgatccccag gcaggaatac
      181 gaagatgcgc tgggcaaaac ggaagaggcc gagcagcggg cgagggtggc gcgcgagcgc
      241 tacgagacct tgctggccgg cacgcgtcca gaagatttgg aacgggtgaa acagcgccta
      301 gccgaagcgg aggcacggct tcgacagttg cgcagcggct ttcgcaagga agagattgct
      361 caggcaaagt cggcggtgga agcggcgcaa gccaaagtgc agtggattcg cactcagctc
      421 gacgagacgg tgatcaaggc gccggcggat gcgctggtgg aaaaactcga tatcgaagcc
      481 ggtgatctcg tcagcgccgg caagccggtg gccctgctgg ttcgcaccgg cagtctctgg
      541 gtgcgcactt atcttccgca agcgcaactt cgtttcgcgc gacccggcac gaaagccaat
      601 gtccgagtgg atgtgtatcc aggaaccgat ttcgacggcg tgatccggcg catccactta
      661 gaaaacgact cgacgccgag taaactactc agccgcgaag aacgagcatt gcaggttgtt
      721 cagactgaag tcatggttgc tgacccggac aacatgttac ggcctggcat gaatgccgac
      781 gtcatcattg ccaaaaacga cgcccgtacg actgcgcagg cgcaaccgga gctgcgcgct
      841 gattgaacac tcgcgactca tgattatgaa gcgggctgcc gcgcaaccat cggctggccc
      901 cgcggcagca ggcatgccat caatcccacg ctgataaatg acaggatgcc gaggatccag
      961 aaaatcgtcg gcagctccgc gctgaccaac agtaacccga caattgatgt cgaggtcgtt
     1021 ttcgacacct gctgaatcaa acgatcgaag gacacgatcc cgccacgaag ctccgccgga
     1081 gcattctgag tcagcagact tttctgcatc ggtgaaatga ccccgttggc gagaccgtaa
     1141 aagaacagcg cgcagcccac cagccaaggc ccatgaataa agggcacggc gctgacggcg
     1201 gcaccgctga ccacgaacga caaaaatagg atattcgctt tgtcgtgccc cgcggcgatg
     1261 cgcccggcct ggctggaggt gatcatcgcc ccaacggaaa aaaagatata gaggagtccc
     1321 gcggtggcgg tcgaaacatt ctgggtgcga accagaaaga gcggaaaata ggtgagaaaa
     1381 ccatagtcga gaaaaaaccg tagaaaaccg gccgagaatg cgatcaccag acgcggatgg
     1441 cgcgtcaggc ggagaatatc ggccacgtag gcggtcgctt tatcgccggt cttgcccttg
     1501 gtttccggca tccaaatcaa agcgatcaaa ccaagcggaa tcgcgagcgc atagcagaca
     1561 aagggccaga accacgcgat cgcggcaagc acaccgccga ggggcggaaa tatgaagtag
     1621 ccgatgcgat cgagaaagac ctttaatcct tgaccgccaa tttcgttgtc gccttccaag
     1681 agatcgccga tgagcacgat ggtaagcgga atcaccgcgc taaagccgac tccttgtagc
     1741 gtgcgcagcg caagcagcca accgaagctc ggcgcgaacg ccatcgcggt gccgaaaaga
     1801 ccgaacgtga ttaagccaaa gccgagcagc aagcggcggc catagagatc cgcaacggcg
     1861 ccaaaaatcg gcgacaaaac gatcgcgggc gcggtaaaca gcgtcatgat ccaggagagt
     1921 tgcgcatcgc tgatgttaaa cggctgagtc agcgcgggta gcgccggctg aagaaaattg
     1981 acgcccatca tgagcgcgat actcgaggca tagacaatgc tcagtgcgcg cgccttggcc
     2041 ggagggagtt tttccatgcc gaaaagcgag gtgatcgcaa tcatccccgc aacgtagtag
     2101 aagcaccggc ggcagtcaag cgtgtagggc gcagcatgct gcgcccaatc aacgggcgcg
     2161 gtggtcagtg ttacgcggcc atgctatggc taacagaatt cgccgcccag gagggtttat
     2221 gccgctggat atgcgcacat ggatcgatca attggagaaa gccggcctcc tcgccaggat
     2281 cgccaagccg gtggatccac gcacgcaaat gggcgcgctc ttgtggcaag cccgcgatcg
     2341 agcgttgttg tttgagaacc ttcccggcta tccgggctgg cgctgcttgg gtcaggcgcc
     2401 aggcgatgtt tcacttgcgc cagcggcctt tgggacggag cgcgatgaga tgatccctga
     2461 gttcgttcgc cgtacggaaa agctcggcac cacacgattg gtcgaaaccg gtccggtgca
     2521 acaaaaagtt ctcatcggcg acgaagtcga tattaccaag atgccgattc atcaggcggg
     2581 catccgcgat ggcggcccgt tcatcggctc gggcttgat
//
LOCUS       DASULZ010000204         2567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1344650, whole genome shotgun sequence.
ACCESSION   DASULZ010000204 DASULZ010000000
VERSION     DASULZ010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344650"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VFY96_04200"
     CDS             <1..482
                     /locus_tag="VFY96_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605692.1"
                     /translation="IDAANLDSSFSRKLKQKGFSVLTDASKVNLPIVSQGIVVTAEYL
                     HKQPRVVEGVLQALIEGLAFTVAPANKALVLKTLTKKLGVMDPSALEEGYQEILTGLD
                     RKPYPSVEGMKNIQRLLQSREPRLANVKIENVVDDELMRKLDSSGFIDRVYRGYGIK"
     gene            complement(775..975)
                     /locus_tag="VFY96_04205"
     CDS             complement(775..975)
                     /locus_tag="VFY96_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605693.1"
                     /translation="MYRILCDRQPARELGSGLTWLKEDGKIVEFSTTEEAGAMVKKLT
                     EGRRAATASTVTYTAKKYNADD"
     gene            1152..2096
                     /locus_tag="VFY96_04210"
     CDS             1152..2096
                     /locus_tag="VFY96_04210"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605694.1"
                     /translation="MKRIAAHGILVLALWCFGAQPAATQSTTLHVGFNGFYGAAPLYV
                     AQDAGIFRKHGFTLELVFIAGGSLSTQALIGKSLDLLQTGGPPFLNAYVRGARLKIIA
                     GVTNILPYVLITASRITSSEQLRGKKIGISRFGSNTDFVVRFALGHLGLTPADVTVLQ
                     VGGSQARFVALKAGTIDATVLSPEEALAAQKLGMNTLLDFVAKGVEFPHVAIVAREEY
                     LQTQAPLVRRFMTAYLEGIRFFKTRKEESVRKMMVLSRLNDREIAEKAFDVYTRSLPD
                     DGRPTIKGLESVLTDFAREEPKARGLTVAQIVDLSFLP"
     gene            2218..2478
                     /locus_tag="VFY96_04215"
     CDS             2218..2478
                     /locus_tag="VFY96_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605695.1"
                     /translation="MRRISKLFAALLLVMNLATACSTQKTITTHSVQNDPHTGEPVTI
                     EKTTTTDNSGAGAAVWSGTLSLLEMVILLPIRAAAILIGAIL"
BASE COUNT          574 a          691 c          720 g          582 t
ORIGIN      
        1 tcatcgatgc cgcgaatctg gatagcagct tcagccgcaa gctcaagcaa aaaggctttt
       61 ccgttcttac tgatgcaagc aaagtcaatc ttccgatcgt gagccagggt attgtcgtga
      121 ccgcggaata tttacacaaa caacctcgcg tggtggaggg agttctccag gcattgatcg
      181 aagggttggc ctttacggtc gcgccggcca ataaagcgct ggtgctaaag accttgacga
      241 agaaactggg cgtcatggat ccctcggcct tggaagaagg ttatcaggaa atcctcaccg
      301 gtctggaccg caagccttac ccatctgtcg agggcatgaa aaatatccaa cggctgttac
      361 aatcgagaga accgcgcctt gcaaacgtca agatcgagaa tgttgtcgac gatgagttga
      421 tgcgaaaact cgattcgagt ggttttatcg atcgagttta tcgcggctac ggcatcaagt
      481 aagacgcagg aacgagggcg cggcggcgat tggcgctttg agtggaaagc tgaaagagag
      541 gaaactatgc ccgcctcatc gacgttagca ttcatcatcg ctgcgctcgc ggtattcacg
      601 gctccgacgc caagcgtcgc ggcggaatct tcctgtcccg ccatcatcga tccaaattct
      661 ccaatttgat ggacgagccg gtcacgctct cccagttcag cggtaaagtg ctgctgatcg
      721 tcaatatgcg ggcgagtaag cagcgccgcc ctagcgcgga gtgtattagg cgaactaatc
      781 gtcggcgtta tattttttcg ccgtatacgt cactgtcgaa gctgtcgcag ctctgcgccc
      841 ctcagttaat ttttttacca tagccccggc ttcttcggtc gtagaaaact cgacgatctt
      901 gccgtcttcc ttgagccagg tcaggcctga accgagttcg cgagccggct ggcggtcgca
      961 caaaattctg tacatataaa cctcctaatc gaacctcact acggaatcgg attgggtttg
     1021 agagatttgc ggaaaaggaa cgataggact tttgagctgc gtaagcaaac tgagcaaatg
     1081 aagcggacaa tttatcgtct tgaccgacac cggtgttagg gagcagtcta ggtgccagta
     1141 gggggtcggg tatgaaacgg atcgccgccc atggaatctt ggtcctcgct ctctggtgct
     1201 ttggcgcgca gcccgcggcg actcagagca ccactctgca cgtcggcttc aacggctttt
     1261 acggcgcagc gccgctctac gtcgctcagg acgccggcat cttccgcaaa catggcttta
     1321 ctctggaact ggttttcatc gccggcggtt cgctttcgac acaggcgttg atcgggaaaa
     1381 gtctcgacct gcttcagacc ggcggcccgc ctttcctgaa cgcctacgtg agaggggcgc
     1441 ggctcaagat catcgcgggc gttacgaata tcctgcccta cgtcttgata acagcgagtc
     1501 gcattaccag ctcggagcag ttgcgcggca aaaagatcgg catcagccgt ttcggcagca
     1561 acaccgactt cgtggtgcgg ttcgcgctcg ggcatctggg tttgaccccg gcggatgtga
     1621 cggtgctcca ggtaggcggc tcccaggccc ggtttgtcgc gctcaaagcc ggaaccatcg
     1681 acgcaacggt cttgagcccg gaggaagcgc tggcggcgca gaagctcggt atgaacacgc
     1741 tcctcgactt tgtcgccaag ggcgtcgaat ttcctcatgt ggccatcgtg gcccgggagg
     1801 aatacttgca gacgcaggcg ccgctcgtga ggcgcttcat gaccgcctat ctagaaggga
     1861 tccgtttttt caaaacgcgg aaggaggagt cggttcgtaa aatgatggtg ctcagccgat
     1921 tgaacgaccg ggagatcgcg gagaaggcgt ttgacgtcta tacgcgctcg ctaccagacg
     1981 atggccggcc cacgatcaag ggattggaat cggtcctgac ggactttgcc cgggaggagc
     2041 cgaaggccag aggcttgacc gtcgcgcaga tcgtcgatct cagcttcctg ccgtaaagag
     2101 agtctcggtc tgaagaaggt ctcgttgtca ggattgccaa gttgcccgca tcttcgctgg
     2161 cctactatcg gccagcttgc ttttaatcgt tcgagcttgg attgatggag aaacgttatg
     2221 cgcaggattt caaaactgtt cgccgctttg cttctcgtca tgaatctggc tactgcgtgc
     2281 tcgacccaaa aaaccattac aacccattct gtgcaaaacg acccgcacac aggcgagccc
     2341 gtgacaatag aaaagaccac tactacagac aattctggtg caggcgcagc agtttggagt
     2401 ggaactctga gtctgctgga aatggtgatc ttgttaccga tccgtgcggc cgccatcctg
     2461 atcggcgcaa ttttgtgata taaagcgtcc gttttatccg tataggacca aatggatata
     2521 gcggctcaac tccaataaat attttttttg atccacttca ccgccaa
//
LOCUS       DASULZ010000205         4614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_938536, whole genome shotgun sequence.
ACCESSION   DASULZ010000205 DASULZ010000000
VERSION     DASULZ010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938536"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VFY96_04220"
     CDS             <1..215
                     /locus_tag="VFY96_04220"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX5605696.1"
                     /translation="IIDLDILLYDSAVVKKKTLKIPHPEMPKRKFVLIPLSEIAPQVI
                     HPELGVTISELLINVKDDKKIHLFHS"
     gene            complement(620..2041)
                     /gene="mpl"
                     /locus_tag="VFY96_04225"
     CDS             complement(620..2041)
                     /gene="mpl"
                     /locus_tag="VFY96_04225"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEX5605697.1"
                     /translation="MNDIPPSGSHIHLIAICGVGMASLAGLLQARGYQVTGSDQNIYP
                     PMSSYLAEIGINILPGFHPDHLMPTPDLVVVGNAVSRNNPEADVALNQNIPYISFPQA
                     LGRFLIGSRSSLVVAGTHGKTTTTALAAWVLTGAGFDPGFFIGGVPLNFGSGWRAGSG
                     EHVVIEGDEYDTAFFDKGPKFLHYRPRNVILTSVEFDHADIYRDLNHVKSAFRKLIEI
                     IPDDGSLVVCGDYPDAKEIAVAARCPRVSYGHSAESDWKAINIEFREGRTFFEPCYHG
                     KSEGRLEVDVIGRHNVMNALAVYAMGRYLGIARQELRCAFPGFAGVKRRQEKRGERRG
                     VLVIDDFAHHPTAVRETIEAVRSAYCGRRVWAVFEPRSNTSKRNIFEQEFAKALALAD
                     QVVLANVYQPEKVPETERLSVETVAGLINRASAAPKARVIGGAAAIASHLAGDAEPGD
                     VVLVMSNGGFDSVHEKILDALAR"
     gene            complement(2038..3177)
                     /locus_tag="VFY96_04230"
     CDS             complement(2038..3177)
                     /locus_tag="VFY96_04230"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5605698.1"
                     /translation="MKSAIDFRGVEESFERAIAEGVFPGAVLLIGKGDAIVYERAFGH
                     RSLLPQKTPMLTDTIFDLASLTKALATSVALMLLVREKKVRLDDQVTRVIPMYGVFGK
                     SLTTFRHLLNHSAGLPAWKPYFEEVIKSEKAGRINFVASRAAKNYVYEQIHRERPESA
                     PGTRTVYSDLGFIVLGEVVEILTGNPLDRFCQEKIFKPLELRSAGFVDLTQIRSRRLQ
                     PVEERIAPTEECPWRKKILCGEVHDDNAYAMGGVAGHAGLFSSAHDIHVLIGRLSQCL
                     LGKDNFLPQTLLREFLSRDDASKDGSFALGWDTPSAAKSASGTHFSTRSVGHLGFTGC
                     SVWWDLEKNCHVVLLTNRVHPSRKNEKIKEFRPHVHDLIMKALFA"
     gene            complement(3233..4273)
                     /locus_tag="VFY96_04235"
     CDS             complement(3233..4273)
                     /locus_tag="VFY96_04235"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEX5605699.1"
                     /translation="MLCHQGFFANIAFPVGIVARALKVIHRRLRIGGVVSTANQVKSH
                     LTVQKPPRLRRGGRIGVIAPAGRIDRGEVESGIAAIRAEGFQVELGRNLFETKGYLAG
                     DPQCRARDLLDFFHRSDIDAIFCARGGFGSIQLLPYLSAHLQADAKIFVGYSDITILL
                     NWLRQFHGMVTFHAPMVAMDLARGLSAQSQAHFWPLLSGDLQSWKMDLTEAIRSGRAE
                     GDMIGGCLSLLVTTLGTPYEINTAGKLLFLEDVGEKPYRIERMMTQLLMAGKLDQPAG
                     VLLGDFTRCDDAGPRDLREIITELFRHVSYPVVMGMRAGHGDENLALPFGVRMALDGG
                     RATLELLESPVR"
     gene            4418..>4614
                     /locus_tag="VFY96_04240"
     CDS             4418..>4614
                     /locus_tag="VFY96_04240"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HEX5605700.1"
                     /translation="MKIKPEQIEQFAVLLLKNYRDKDVITAKADEGALKKAIVEIITK
                     NFAEEDAIEEEARKVLAAHPQA"
BASE COUNT         1104 a         1327 c         1190 g          993 t
ORIGIN      
        1 gcatcatcga tctggacatt ctgctttacg attcggcagt cgtgaaaaaa aagactttaa
       61 aaattccgca tccggaaatg ccgaagcgca aatttgttct tattccgcta agtgaaattg
      121 ccccgcaggt gattcatccc gagctgggtg tgacgatttc cgagcttctg atcaacgtca
      181 aagacgataa gaaaattcac ttgtttcact cttaaccggt cggcgccctg atcctggcga
      241 actcatagcg agaaacgcaa ttagctcgat ctgaagcgta cagacctcga tcatttgtcg
      301 tcggccgacg ctcgcgcgat agggattttg gcacgactct atcaccccgt cgacaaaccc
      361 ccgctgaaat gcgaatactt ataccagctc aatgattctc tttcgcctga ttataatctt
      421 cctgatttgt tacctcctaa ttatcgcgct gaaatcgttt ttccggccat ccaaaatcag
      481 atcagcaaat cgtaaaaact tcaccgaaga cagtgaggat atggtgctcg atccgcagtg
      541 ccaaagctat gtcccgaaag caagcgcggt ggccgagtcc ggcaagtttt tttgtagtcg
      601 cgagtgtgcg cagcgatatc tatcgcgcta gagcgtcgag aattttttca tgcaccgagt
      661 caaagccgcc attggacatc accaggacca catcgcccgg ttccgcatcg ccggctaggt
      721 gcgaagcaat cgccgctgcg ccgccgatca cccgagcctt gggcgcggcg ctggctcgat
      781 taatcagccc ggcgactgtt tccactgaaa gcctctcggt ttcgggcacc ttttcaggct
      841 gatagacgtt ggcgagaacc acctggtcgg cgagcgcaag tgccttggca aattcttgtt
      901 caaaaatatt tcgtttgctg gtgttgctgc gcggttcgaa aaccgcccag actctgcgcc
      961 cgcaataagc tgacctgacg gcctcaatcg tctcgcgcac ggcagtggga tgatgcgcaa
     1021 aatcgtcgat gaccaaaacg ccccgccgct cgccgcgttt ctcttgacgc cgcttaacgc
     1081 ctgcaaagcc gggaaaagcg cagcgcaact cttgcctggc aattcctaag taccgtccca
     1141 tcgcataaac cgcgagcgcg ttcatgacgt tgtgccgtcc gatgacgtca acttccaagc
     1201 ggccctcgga cttgccgtgg tagcacggct cgaaaaaagt tcgcccctca cggaattcga
     1261 tattgatcgc cttccagtcg ctctccgcgc tgtgaccgta actcacacgc gggcagcgtg
     1321 ccgcaacagc gatctctttt gcgtccggat aatcaccgca caccaccagc gacccgtcat
     1381 cgggaataat ctcgatgagc ttacgaaatg cgctctttac gtgattgaga tcacgataga
     1441 tgtccgcgtg gtcgaattcg acgctggtga gaataacgtt acgtgggcgg tagtgcaaaa
     1501 attttggccc tttgtcgaaa aatgccgtgt catactcatc tccttcaatg acgacatgct
     1561 cgccgctgcc ggcacgccag ccgctgccga aattcagcgg cacgccgccg atgaaaaaac
     1621 ccggatcgaa tcctgcgccc gtaagaaccc atgccgccaa cgccgtggtc gtcgtcttcc
     1681 cgtgggtgcc cgccacgacc agtgagcttc gcgagccgat tagaaaccgg cccaatgcct
     1741 gagggaatga gatatatggg atattttggt tcaaggcgac gtcggcctca ggattattgc
     1801 gcgacacggc attgccaacg accacgagat ctggcgtcgg catcaaatga tcggggtgaa
     1861 aacccggcaa aatgttgatc ccaatctccg cgaggtaact gctcatgggt ggataaatat
     1921 tctggtcgga tcccgttacc tgatacccgc gcgcctgtaa cagacccgca agtgaggcca
     1981 ttccgacacc gcatatcgcg attagatgaa tatgactgcc actcggaggt atatcgttca
     2041 tgcgaacagc gctttcatga tcaaatcgtg cacgtgcggt cggaattctt tgatcttttc
     2101 gttttttcgc gacggatgaa cccgattggt cagcagcacc acatggcaat ttttttcgag
     2161 gtcccaccaa acagagcaac cggtaaatcc caagtgacca accgatcgcg ttgaaaaatg
     2221 ggtcccgctg gcggacttag cagccgaggg cgtgtcccaa ccaagagcga agctcccgtc
     2281 cttcgaagca tcgtcgcggc tgaggaattc tcggagcaaa gtttgcggca agaagttgtc
     2341 ctttccgagc aagcactggc tcaggcgacc gattaagaca tggatatcgt gcgccgacga
     2401 aaaaagccct gcgtgtcccg ccaccccgcc catcgcgtag gcgttgtcat catgcacctc
     2461 gccgcagaga attttttttc gccaggggca ctcttcagtg ggcgcaatcc tttcttccac
     2521 cggctgcaat cgccgggacc gaatctgagt gagatcgaca aaaccggccg agcgaagctc
     2581 caaaggcttg aagatttttt cctgacagaa gcgatcaagt gggttaccag ttagaatctc
     2641 gacaacctcg cccagaacaa tgaagccgag gtcgctataa acggtccgag ttcccggcgc
     2701 gctttccggt cgttcacggt gaatttgctc gtagacgtaa ttcttcgctg cacggctcgc
     2761 gacaaagtta attcgtcctg ccttttcact ctttatgact tcttcgaaat agggcttcca
     2821 ggcgggtaag cctgccgaat gattcagtag atggcgaaat gtcgtgagac tcttgccgaa
     2881 aactccgtac atcgggatta cccgagtgac ttggtcgtcc aagcgcactt ttttctcacg
     2941 caccagaagc atgagggcga cggaggtcgc tagggctttg gtgagcgatg ccagatcaaa
     3001 tatcgtgtcg gtcagcatcg gcgttttctg tggcaggaga gaacgatgtc cgaaggctcg
     3061 ctcatagacg atcgcgtccc ccttgccgat taagagcacc gcgccaggaa atactccctc
     3121 agcgatagct ctctcaaaac tctcttcaac gccacggaaa tcgatggcag atttcattcg
     3181 gatggtgcga gaaatatgac aaagaggcgg agcacagaag ttattcagta cttcagcgta
     3241 ccggagattc gagcagctcc aaagtggcgc gacctccgtc gagcgccatt cgtacgccaa
     3301 acggcaatgc caggttttcg tcaccgtgtc ccgccctcat acccataacc acggggtaag
     3361 atacgtgacg aaatagctcc gtaatgattt ccctcaaatc gcgtgggccc gcatcatcgc
     3421 agcgggtgaa atcgcccaac aagacaccgg ctggctgatc caatttccca gccatcagaa
     3481 gctgcgtcat catgcgctcg atgcgatacg gcttttcgcc gacatcctcc aagaagagca
     3541 actttcccgc cgtattgatc tcatagggag tgccaagcgt agtcaccagt aaggagagac
     3601 aaccgccgat catgtcgcct tcggcgcggc ctgaccgtat ggcctcggtc aaatccatct
     3661 tccagctctg gaggtcgccg gaaaggagcg gccagaagtg cgcctgcgac tgcgcgctca
     3721 ggccgcgggc gaggtccatg gcgaccatcg gagcatgaaa tgtgaccata ccatggaatt
     3781 gcctcagcca gttcaggaga attgtgatat cactgtaacc gacaaaaatt ttggcatccg
     3841 cctgcagatg cgcgctcaag tatggcagca gctgaatcga gccgaatcca ccacgcgcgc
     3901 aaaaaattgc gtcgatatcg ctgcggtgaa aaaaatccag cagatctcgg gcgcgacact
     3961 gaggatcacc ggcaagataa cctttggtct cgaacaaatt cctgcccagc tcgacttgga
     4021 acccttccgc acgaatggcc gcaatccctg attcgacctc gccgcgatcg attctaccgg
     4081 cgggggcgat gacaccgata cgtccgccac gcctcaaccg cgggggcttt tgcacagtta
     4141 aatgcgattt tacctggttc gctgtcgaga ccactccacc gatccttagc cgcctgtgaa
     4201 taaccttaag cgcgcgcgcg acgattccga ctggaaatgc aatattggca aaaaacccct
     4261 gatggcacaa catttttttt cctgttgcgg tgctgtcacg ggcgaaccgc cgcgcgttga
     4321 cttcccttca tcggccagct agatagcaaa aagatccaca agcatgccgg cccaggcacc
     4381 ggacgataga cagtttatcc acaggatctg aacagccatg aagataaagc ccgagcagat
     4441 cgaacaattt gccgtgttgt tgctgaaaaa ctaccgtgat aaggatgtga tcaccgccaa
     4501 ggcggatgag ggcgcgctca aaaaggcgat cgtagagatc atcacgaaga atttcgccga
     4561 agaggacgcg atcgaagaag aagctcggaa ggtgctcgcg gcccaccccc aggc
//
LOCUS       DASULZ010000206         3471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_25902, whole genome shotgun sequence.
ACCESSION   DASULZ010000206 DASULZ010000000
VERSION     DASULZ010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25902"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VFY96_04245"
     CDS             complement(<1..299)
                     /locus_tag="VFY96_04245"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605701.1"
                     /translation="MKHRILPNNKFVITWREISAAVAAIRKSFRPSRMSGVRRKGFGN
                     TLESSMDLADIGKTFRRLDYETKVTGRAQYLADMSLPGMCHGKILRSPYPHARIK"
     gene            complement(181..645)
                     /locus_tag="VFY96_04250"
     CDS             complement(181..645)
                     /locus_tag="VFY96_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5605702.1"
                     /translation="MKKTISLTVNGVERNAEVEPRLTLLDLVREHFGLTGAKLGCDVQ
                     VCGACTLLVNGKPISACSMLALDADGCEVLTIEGLSKHNKLHPVQEAFVEFGALQCGY
                     CTSGFILTTKALLDETPHPSEQQIRDYLAGNFCRCGCYQEIIQAVKNVRSEA"
     gene            complement(722..1594)
                     /locus_tag="VFY96_04255"
     CDS             complement(722..1594)
                     /locus_tag="VFY96_04255"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX5605703.1"
                     /translation="MKNFEYLEPTTVAEACALLKQNGGAAKIFAGGAHLTILMKQGLY
                     QPRALINIKKIPELKGFRCNDAGLSIGALVTHRELETSALVRDKFPVICEAEREVANI
                     RVRHMGTVGGNLASGEPLTDLSQIFIALDGTVKVAGSIGERIIPVQELFLDFYTTCLA
                     DDEIITEIMLPLLPKKSGIDYLRFSSSSVVDKPAAGVAVRLTLEPGNEISQLARVVLG
                     CVGATPVRARRAEEILTGQRVSLDAAVEAGKAASQECDPISDLRGSAEYKRAIVGTLV
                     KRAAVKAYERAVRN"
     gene            complement(1761..3107)
                     /locus_tag="VFY96_04260"
     CDS             complement(1761..3107)
                     /locus_tag="VFY96_04260"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX5605704.1"
                     /translation="MFEDLRGFLTHLESQRQLLRVKDPIDVKYEIAAGMRKTSDIEGP
                     ALLFENIKGYPGWRVLGGLFATRKLVALGLGVPQEQMLERYLTLEDQRIPPEIVSSAP
                     CKEIRWTGEQIDLSKLPIVTHASKDCGPYVTIGVQVGKDPETGIRNLSIHRMLVLGKD
                     RLSLWAPADHHLGRMILKAEERKKGLEVATAVGVDPAIVVGSQARVPFGIDEFHVAGG
                     LRGAAVKLTKCETIDVEVPAFSEVVIEGITIPGERVADGPYGEYPGCYSEAKQAPVLK
                     VTAITMRQNPIWQTALTGMPVTENHTLIEYGNAAVVYREVKKVVPEVRGVNMTPGGTF
                     RHHVVVSIKKRAENEGRNVILALLSMGIGLKQVIVVDDDIDPFDPMQVDWAMATRFQA
                     DKDAIIIPRIACSTLDPSCPEARVTAGLGIDATAPMKDRWRFEKVEIPGVDKVKYI"
     gene            complement(3241..>3471)
                     /locus_tag="VFY96_04265"
     CDS             complement(3241..>3471)
                     /locus_tag="VFY96_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605705.1"
                     /translation="YAEAKDYFNHYYGPSYPPESLIKVWLAHGPPKDCARLVQDWIDM
                     GITTPVLRFTGKDQLLQVRRFIDEVLPLLRLK"
BASE COUNT          735 a         1038 c          883 g          815 t
ORIGIN      
        1 cttttgattc gcgcgtgcgg atacgggctg cgcaggattt tcccatgaca catcccgggc
       61 agactcatat cggcgagata ttgtgcacga ccggtcactt tggtttcata gtccagccgc
      121 cgaaaggtct ttccgatatc agctaagtcc attgacgatt ccagagtatt gccgaacccc
      181 ttacgcctca ctcctgacat tcttgacggc ctgaatgatt tcctgatagc agccacagcg
      241 gcagaaattt cccgccaggt aatcacgaat ttgttgttcg gaaggatgcg gtgtttcatc
      301 gagcagcgct ttggtcgtga gaataaaccc ggaggtgcaa tagccgcact gtagcgcgcc
      361 aaattcgacg aatgcctcct gcacaggatg aagcttgttg tgtttgctca agccttcgat
      421 ggtcaaaact tcacagccat cggcatccag cgccagcatt gagcaagcgc tgatcggttt
      481 accgttgacc aatagggtgc aagctccgca aacttggaca tcacagccga gctttgctcc
      541 cgtcagccca aaatgctcgc gcacgagatc caaaagcgtc aggcgcggct ctacctcagc
      601 gttcctttct acgccgttca ctgtaagcga gatcgtcttc ttcatgcctt gtttgaactc
      661 ttcaatcgaa aattcaaaac ctgtccggag ccgagtcgaa ggatcggaaa tctaaaattt
      721 cctagtttct caccgcccgc tcatacgctt tcacggctgc ccgtttcacc aaagtcccga
      781 caatcgcgcg tttgtactct gcagatccac gcaggtcgct tatgggatcg cactcctgtg
      841 atgctgcctt gccggcttca acagcagcat ccagggatac ccgttgcccg gttaaaatct
      901 cttccgctct tctagcgcgg accggcgtcg caccgacgca ccccagtacg acgcgcgcga
      961 gttggctaat ttcgttgcca ggctcgagtg tcagcctcac cgccacccct gccgccggtt
     1021 tatcgaccac cgaactgctg gagaatcgca ggtagtcaat tcccgatttt tttggcagca
     1081 gcggtagcat gatttccgtg atgatctcgt catccgccaa acaggtcgtg taaaaatcga
     1141 ggaacaattc ttgaacggga atgatacgct ctccgattga gccggcgact ttcactgtgc
     1201 catcgagcgc aatgaagatc tgcgacaagt cagtaagcgg ctcgcccgat gcgagatttc
     1261 cgccgaccgt tcccatatgg cgcactcgaa tgttggcgac ttctctctcc gcctcacaaa
     1321 taacgggaaa tttgtcacgc accagcgctg aggtttcgag ctcgcgatgc gtcaccagcg
     1381 cgccgatcga cagtcccgca tcgttgcagc gaaatccctt gagctcggga attttcttga
     1441 tgttgatgag cgctcggggc tgataaagcc cctgcttcat caggatcgtg agatgcgcac
     1501 caccggcaaa aatcttcgct gcgccgccgt tctgtttcag cagcgcgcac gcttcagcga
     1561 cggtagtggg ttcgaggtac tcgaaatttt tcatgggcta gcttgagttg attggctaca
     1621 tcacggaatt cgtcattccg gccaagctgg cgtagctagc gcgagccgga atccaggttt
     1681 cgaaggacca ctggattcgc gcatccgcgg gaatgacaaa gtatagaact aatattctcc
     1741 acgattctca ggccgaagct tcaaatatat ttcaccttat cgacgcccgg aatctcgacc
     1801 ttctcgaatc tccagcggtc cttcatgggt gccgtcgcat cgatgcccaa tccggcggtg
     1861 acccgcgctt ccggacaaga tggatccaag gtcgagcagg cgatgcgcgg aattatgatc
     1921 gcatccttat cggcttggaa acgagtcgcc attgcccaat ccacctgcat gggatcgaac
     1981 ggatcgatat catcgtcgac gacaatcacc tgtttaagac cgatgcccat ggagagcagc
     2041 gcgagaatga cgttccggcc ttcgttctcc gctcgcttct tgatcgaaac caccacgtga
     2101 tggcgaaagg ttccccccgg agtcatatta acgccgcgca cttccggcac gacctttttc
     2161 acctcccggt acaccaccgc agcattccca tactcgatca gcgtatggtt ctccgtcacc
     2221 ggcattccgg tcagcgcggt ttgccagata gggttctgcc gcattgtgat cgccgtgact
     2281 tttagcaccg gagcctgttt ggcttcgctg taacatcccg gatattcgcc gtatggaccg
     2341 tcggcgaccc gctcaccggg gatggtgatt ccctcgatca ccacttcgga gaacgcggga
     2401 acttccacgt cgatggtttc acacttcgtc agcttcacgg ctgcgccacg caatcctccg
     2461 gcgacgtgaa attcatcgat accaaacggc actcgcgcct gagagccgac gacaattgcc
     2521 ggatcgacgc ctaccgccgt ggcgacttcc aggccttttt tcctttcctc cgcttttaga
     2581 atcatcctgc caaggtggtg atccgccggc gcccagagag agagtcggtc cttgccgagc
     2641 acaagcatcc ggtggatcga gaggttgcgg atgccggtct cgggatcctt gccgacctgc
     2701 acgccgatcg taacgtaagg cccgcaatcc ttgctcgcgt gcgtcacgat cggcagcttg
     2761 gagagatcga tttgttcgcc ggtccatctg atttccttgc agggtgcgct ggagacgatc
     2821 tccggcggaa tgcgttgatc ttccagtgtc aaatagcgct cgagcatctg ctcttgtggc
     2881 acgccgagtc caagtgcaac taattttcgc gtcgcaaaca atcctccgag cacgcgccag
     2941 ccggggtaac ctttgatatt ctcgaaaagc agcgccggtc cttcaatatc ggaggttttg
     3001 cgcatccccg cggcgatctc gtacttgacg tcaatcggat ccttcacccg cagcagctgc
     3061 cgttgacttt ccaggtgagt caaaaaacct ctcagatctt caaacatgat tgttttcctg
     3121 tacgaaacag atccttcact cggttcagga tgacgaccgc tgccattctg agcaaagcga
     3181 agcgcctcgc cgaattgttc gggttcggca ggtggttctg ttttatcagc gtcgcagccg
     3241 tcacttcaac cgcaacagcg gtagtacctc atcgataaat cgtctcacct gaagcaattg
     3301 gtccttcccc gtgaaacgga gcaccggcgt cgtaatgccc atgtcgatcc agtcctgaac
     3361 caagcgggcg caatcttttg gcggtccatg agcgagccaa accttgatca aactctcggg
     3421 cggatagctc ggcccataat agtggttgaa gtaatctttc gcctcggcat a
//
LOCUS       DASULZ010000207         5219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_431494, whole genome shotgun sequence.
ACCESSION   DASULZ010000207 DASULZ010000000
VERSION     DASULZ010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431494"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            444..1652
                     /locus_tag="VFY96_04270"
     CDS             444..1652
                     /locus_tag="VFY96_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5605706.1"
                     /translation="MDNTGPAEAIALAHRDVAPSLSKIAVSLRGLWKTYPGSNKPAVQ
                     NLSLDVHDGEIVTLLGPSGCGKTTTLRIVAGLETPDAGDIFFGDQAVVVSARRFSLPP
                     NKRNVGMVFQSYAIWPHMTVAQNVAFPLKARRVPKREIIERVQRALDLVGLSGFEDRP
                     GPLLSGGQQQRVAFARALVTEPRVLLLDEPFSNLDAKLREQMRISVKLLQKRLSVAML
                     FVTHDQIEALSLSNRIALMNFGVVQQQGEPRILYESPVNEFVRDFVGRTVLFKGQVQS
                     SNPSGQIAIAIAGGRDCVVFGRSYNPNGIKNGDPVFIAVRPEDVEIQPALGATMPSGS
                     SAGTVKTTLFIGERIEYQVEVDDQGLMMIYGERHQPIDEGKRVWLKLRPEGHSAWSTA
                     WSHKGEQENL"
     gene            1748..2854
                     /locus_tag="VFY96_04275"
     CDS             1748..2854
                     /locus_tag="VFY96_04275"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5605707.1"
                     /translation="MLSFSAGLAAAPANFEEEWAKLSAAAKQEATLSIASGGAPSRQY
                     RPVIDVFSKKFGVKVEVSTGNATDTVNRVLAERKAGRYIMDVALISSRENNQRLVPSE
                     SLVPFTPLLIHPEVVDTSGWYLGRHWYADKASAYVFIYHVTKEDQYESWYNTDKLKEA
                     DIVTIKKQTDFFDPRWKGKILGQGMGDPSGIRQMIDSYFEPDRGPEWVRTYLLNAGIT
                     FSDDRRILETWLVGGRFPLQAVATGTEELTALAKKGLPIKQIFLPKQVGLVRAGGSGC
                     CISVFSNAPHPNTAKLFVNWFLSKEGQTLTHTLVPNIDRSSLRNDIPFGEVTPDQRRK
                     PGVEYAFPDADPKFGARQEEAQKWIYKIWETRQK"
     gene            2990..4792
                     /locus_tag="VFY96_04280"
     CDS             2990..4792
                     /locus_tag="VFY96_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5605708.1"
                     /translation="MDQSPTIDELSVPQPVAPPLLTKGHFLMALLLLSLAFLLIWPVI
                     LLLINSFNTANDWFVEPRSWGIRHWINAFQRPGLLTSLGNSLLIWTLTVTVSFPVGVS
                     IAWLLARTKIPFSHTLEFLFWVSYMVPSLPTTIAWITLLDPDIGVVNLALKSLFHLDQ
                     GPFNIFSVPGIIWANLMGHGISIKVMLLTPAFRNMDATLEEAARVGGAGNLRTLFKVT
                     LPLMISPMMTVFALQLLRVFQSFETEYLLGMPIGFFVYSTKIFTLIRNQIPNYGEATV
                     LASLTLLIIALIIPLQRWILARRRYTTITGSFRPGLIDLGNWNYIAFAAIAFLLALLT
                     VGPLAILVLGSFMQRIGYFVLGFTLEHWRFVLSDPVFVKALRTTLVLSISAAICSPLL
                     FSVLAYILVRTRLPGRAALDFMIWSAGAIPGILAGLGLLWVFIGTPGLNFLFGTIWAL
                     IIVILLQGKTTGVNIMKGVLVQVGADMEEAARVSGAGWLRTYVMIWLPLLTPSLILLA
                     VMNFVSAAGATSSIILLASRDTMTLSLMALELSSVAVSNREAASIISIFIIALTVSGA
                     LLVRYFGRHLGVRHEMQVDPNAPQVRRSAKIAAA"
BASE COUNT         1121 a         1423 c         1468 g         1207 t
ORIGIN      
        1 tgagaacgcc actgaggacg acacgattct gttctctatc gacgattggc ccgcgatgaa
       61 aaagcttggc ttctatatga aagaagagcc gaagggattt tgaaaagttc cttcgcacaa
      121 agcctcgcag gcaggtgatc cctcgattgc gttcggcaca ggctccgaaa accgctcgcg
      181 tttagggctg tccggaacga tggttttcgg cacggcctca acccatccta gacactgcag
      241 ttcctcccgt agggtggctt cggaacccgc cgttttcagg cgcgacacgc cgttatcggc
      301 acaaacactt cgatggattc cacgaggcaa caattatccg gatgcgctgc cttccgtgag
      361 ggcgacgggc gggtcgcccg actatgtaaa ggcgcaattg gctgtagcca ctgagattac
      421 tttcgtgtaa gagaagagcc gcaatggaca acaccggtcc agccgaggca attgcgctcg
      481 cccaccgcga tgtcgcgccg tcgctttcga agatcgccgt cagtctgcgc ggtttgtgga
      541 agacctatcc cggtagcaac aagcccgccg tgcagaacct atccctcgat gtacacgacg
      601 gtgagatcgt cactttgctt ggcccgagcg gttgcggcaa gacgactacg ctgcgcatcg
      661 ttgcaggatt ggaaacacct gatgcgggcg acattttttt cggcgatcag gcggtcgtcg
      721 tgagcgcgcg ccggttttcg ttgccaccca acaagcgcaa tgttggcatg gtgtttcagt
      781 cctacgccat ctggccgcat atgaccgtcg ctcagaatgt cgcattcccg ctcaaggctc
      841 gaagggttcc taaacgggaa atcatcgagc gcgtgcagcg cgcgctcgat ctcgtcggat
      901 tgtcaggatt cgaagatcgc cccggccctt tgctaagcgg cgggcaacag cagcgcgtcg
      961 cctttgcgcg agcgctggtc accgaaccgc gagttttatt gctggatgag ccgttcagca
     1021 atctcgatgc gaaactccgc gagcagatgc gcatcagtgt caagctcctg caaaaacgct
     1081 tgagcgtggc gatgctgttc gtcacgcacg atcagatcga ggcgttaagt ctatcgaacc
     1141 gcattgcctt gatgaacttc ggcgtggtgc aacagcaagg cgaaccgcgg attttgtacg
     1201 aatcgccggt caatgaattt gtccgggatt ttgtcggcag aaccgtcttg ttcaaagggc
     1261 aggtgcagag cagcaatcct tcgggccaga ttgcaattgc catagcgggt gggcgcgatt
     1321 gcgtggtgtt cggccgcagt tacaatccaa acggcataaa aaacggcgat cccgtgttca
     1381 tcgccgttcg tccggaggac gtcgagattc agcctgcgct tggcgcgacg atgccgtcgg
     1441 gaagcagcgc tggcacggta aaaacgacgc ttttcattgg cgagagaatc gagtatcagg
     1501 tcgaggtcga cgaccaaggc ctaatgatga tttatggcga gcgacaccaa ccgattgacg
     1561 agggcaaaag agtttggctc aaattacgcc ccgagggaca cagcgcgtgg tcaacggctt
     1621 ggtcgcacaa gggagagcaa gaaaatctat gaaccttcga gcgaaatctg ctggcgagta
     1681 tttgccgcga ggaagacgat cggcccgatc gatccgatcc gtcggatgga tcggatcatt
     1741 atttcttttg ctcagcttca gtgctggttt ggccgctgct ccagccaatt tcgaagagga
     1801 gtgggcgaag ctcagcgctg cggcgaaaca agaagcgacg ctttcgatag cttccggggg
     1861 cgcgccatcg cgtcagtatc gcccagtgat cgatgtgttc tccaaaaaat tcggcgtcaa
     1921 agtcgaagtc tcaaccggta acgccaccga caccgtcaat cgcgtgctcg ccgagcgcaa
     1981 ggcggggaga tacatcatgg acgtggcgtt gatcagcagt cgggagaaca accagaggtt
     2041 ggtaccgtca gaatcgctgg tcccgttcac acctttgctc attcatccgg aagttgtgga
     2101 cacttcgggc tggtacctcg gccggcactg gtatgccgac aaggcatcgg cctatgtatt
     2161 catttaccat gtgaccaaag aggatcagta cgagagttgg tacaatacgg acaagctcaa
     2221 agaagctgat atcgtcacga taaaaaaaca aaccgacttt ttcgacccgc gctggaaagg
     2281 aaaaatcctc ggccagggga tgggcgatcc ttcgggaata cgccagatga tcgacagtta
     2341 tttcgagccc gaccgtgggc cggaatgggt caggacgtat cttctcaatg cgggcatcac
     2401 attttccgat gatcgccgga ttctcgaaac atggcttgtc ggtggaagat tcccgctgca
     2461 ggcggtggct acggggaccg aagaactcac cgcattggca aagaagggac taccaatcaa
     2521 acagattttt ctgcccaagc aagtagggct ggtgcgcgcc ggcggctccg gttgctgcat
     2581 ttctgtcttt tccaacgcgc cccatccgaa cacggccaag ctttttgtga actggttttt
     2641 atccaaagaa ggacagacgt tgacccatac gctggtaccg aacatcgatc gctcatcttt
     2701 gcgcaacgac attccttttg gcgaggtcac gccggaccaa cggcgcaaac ccggcgtcga
     2761 gtacgccttc cccgatgccg atccaaagtt cggagcgcgc caggaagagg cgcaaaaatg
     2821 gatctataag atttgggaaa ccaggcagaa atagaaccca aaagttcaaa cagttcaaac
     2881 cgttcaaata gttcgagatg ttccccctcg atcctccccc gcgccgacgc gggggaggaa
     2941 gcaggtgggg gcttgaacgg ctataacggc ttgaacgatc aaaacgctcg tggaccaatc
     3001 tccaactatt gatgagttaa gcgttccgca gccggttgca ccgccgctcc tgaccaaggg
     3061 ccatttcctc atggctctgt tgctgctatc gcttgctttt cttttgatct ggccggtgat
     3121 cctgcttctg atcaacagtt tcaataccgc caatgattgg tttgtcgagc cgcgatcgtg
     3181 gggcatccgc cactggatca acgcgtttca acgcccgggc cttctgacat ctctgggcaa
     3241 ctccttgctg atctggacgc tcacggtgac ggtcagcttt ccggttggcg tcagcattgc
     3301 ctggctgtta gcacgcacga agatcccttt cagccatacg ctcgaatttc ttttttgggt
     3361 ttcttacatg gtgccgtcgc taccgacgac gatcgcctgg atcacattac tcgatcccga
     3421 catcggcgtc gtcaacctcg cactgaaaag cctctttcat ctcgatcagg ggccattcaa
     3481 tattttcagc gtcccgggaa ttatctgggc gaatctgatg gggcacggca tctcgatcaa
     3541 agttatgctc ttgacgccag cctttcggaa catggatgcg accctcgagg aggccgcgcg
     3601 cgtcggcggc gcgggtaacc tgcgaacgtt gttcaaagtg acgctgccgc tgatgatttc
     3661 cccgatgatg acggtctttg cgttgcagct tctccgggtg tttcagtctt ttgaaacgga
     3721 atatttgctc ggcatgccga tcggattctt cgtttattcg accaagattt tcaccttgat
     3781 tagaaatcaa atccccaatt atggcgaggc tacggttctc gcgagcctca cgctgctgat
     3841 catcgctttg atcattccgc tacagcgctg gattctggcg cgccggcgct acacgacaat
     3901 taccggcagc tttcgtccgg gcctcatcga cctcggcaac tggaattaca ttgcctttgc
     3961 tgcgatcgcg tttctgcttg cgctgctgac ggtgggaccg ctcgcgattc tcgttctcgg
     4021 cagttttatg caacgaatcg gctatttcgt tttgggattt acgctggagc actggcggtt
     4081 tgtgctgagc gatccggttt tcgtaaaagc gcttcggact actttagtgc tctcgatcag
     4141 cgcggcgata tgcagtccat tgcttttttc cgtgctcgct tacattctcg tgcgcacgcg
     4201 actgccgggc cgcgccgcgt tggacttcat gatctggagc gccggcgcaa ttccaggcat
     4261 cctcgcggga ctcggcttgt tatgggtttt tatcggcacg ccggggctga attttctgtt
     4321 tggtacgatt tgggcgctga tcatcgtgat acttctccaa ggcaaaacca ccggagtcaa
     4381 catcatgaaa ggcgtgctgg ttcaggtcgg cgccgatatg gaggaagcgg cgcgcgtctc
     4441 tggcgcggga tggttgagaa cttacgtcat gatttggctg ccgctcttaa cgccgagcct
     4501 gattcttttg gccgtgatga actttgtcag cgccgccggc gcgaccagca gcattattct
     4561 gctcgcttcg cgcgatacta tgacgctctc gttgatggca ctcgagctat cgtcggttgc
     4621 cgtcagcaac cgcgaagccg caagtatcat cagcatcttc attatcgctc tcaccgtaag
     4681 cggagcgttg ttggtgcgct acttcgggcg gcatttaggg gtgcgtcacg agatgcaggt
     4741 ggacccaaac gcgccacaag tgcggcggag cgctaaaatt gctgcggcat gacttggacg
     4801 ccacccgcga aaatgtcccc tgaccggctg ttctgccgaa gaaattccaa ctcaatacag
     4861 atccttcaca tgttcaggat gacaagccgg cgtcattctg agacacaggg tcggtaaaac
     4921 cgattcccga gtagcgcaaa gcgtatcgaa aggtaagcaa tccgtgagcg tagtctctat
     4981 atagtctcgc ggtcgcgaaa aatctgcttt gaagttatgg cgtcgttgct tttttccggg
     5041 cgctgaaata atcaaatcac acatagcgag gaggcttgag atggctgaaa caaatgtcgc
     5101 tgacgcttta gtcgaatact tgagctcgcg aggagtcgtc aatatttttg gtgtgctggc
     5161 gcacacgtcg ttcgcgatcg gcgacgcgat cgcgagacgg ccgcatatga aattcgtca
//
LOCUS       DASULZ010000208         4559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1039383, whole genome shotgun sequence.
ACCESSION   DASULZ010000208 DASULZ010000000
VERSION     DASULZ010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039383"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VFY96_04285"
     CDS             complement(<1..764)
                     /locus_tag="VFY96_04285"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605709.1"
                     /translation="MRRLPTACWTIVLLVFELLGGRSFAADKVRMAYVAPSVSLALPW
                     MAKETGILSKHDLVAEILLITGSPRLVQSLIAGDVDVAFAGVTALTRARVRGADVAIL
                     GASANVSSQKLMVARNSKVRRLEDLKGATIGVSQYGSEADTFTRNALATAGLKPDKDV
                     TILQLGGHPQVAAAMVAGKLDVGALAGLPAWNVEKSGGRLLSSAVELKILSPSGTFAT
                     TRGYIQRKRNVVERLTHAFVETMHFLKTNRAGSITLL"
     gene            1024..2235
                     /locus_tag="VFY96_04290"
     CDS             1024..2235
                     /locus_tag="VFY96_04290"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5605710.1"
                     /translation="MENVTRTGPLTGYRVLDVAGPLGLHCTKLLADMGADVIKIESPS
                     GDESRRIPPFKDDIPHPEKSLYFLHFNTNKRSVTLDIEKPDGRAIFLELARKADVVLE
                     TACPGRMDELGIGYSTLRAQNPGIVYAAITPFGQTGPWRDYKANDMAGVALGNLLYLA
                     GEPGEPPLQPPGEIAYGMASTYGAFGVAVALYHRLDSGKGQYIDVSMHECAGHIAGYF
                     IPNYGYTGEKPARASRKGEETDLYDPYPTKNGYARIFIIPVEQWRRLVDWIGRPPEIS
                     GPEFEKMAYRRKHPDIVTKAIAEFCARLTKEELYDEGQKRRIAVTPINTAGEFVEMPQ
                     TKERGLFVEMEHPVIGKYQQFGVVPRLMETPGAIYRPAPLLGEHNEEIYLDELEMTND
                     DLVALRAEGVI"
     gene            2323..3531
                     /locus_tag="VFY96_04295"
     CDS             2323..3531
                     /locus_tag="VFY96_04295"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5605711.1"
                     /translation="MSKFPLEGIRVIELTTGAAGPTVARVLCEFGAEVIRCETRLRGD
                     GHRGEDPKLWNKKPDFMKLQRGKKSFTVNMQTPRGRELVRELVKKSDVLVENFGLGIL
                     EKWGLDWPQLKEINPQMILIRVKGMGCTGPHAADLTYGPNVGNTMGTTYLWNYPNATT
                     ATAEPRTQHPDFMGGVTGAFGVVCALIQRKKSGFGQWIDSAQQEIGASLLGPKYLDYT
                     VNKREPRPEGNRSLIAAPYGPYQCKGDDRWCVVAVYSEEEWQRFAALLEQSGPKRSEN
                     FATHLQRVRHKDELDQWVTRWTLQYEPYEVMEKLQAIAICAAVVQDVEDQFKRDRQYA
                     ATGYLVRLNEPEAGEVVTENVPVRLSETPGKVRGVAPLMGEHTHAIARNLLGLSDDEI
                     KKLDEEKVLY"
     gene            3642..4484
                     /locus_tag="VFY96_04300"
     CDS             3642..4484
                     /locus_tag="VFY96_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5605712.1"
                     /translation="MSDEPQPLCIYEERDPHIAIIRLNRPEKKNAISRDLYWALDECW
                     QKAKADDDVWSIILTGSGDAFCAGGDLKENLAFAKGEMKGPRSGPRPYSNTRMLQMHK
                     PIIAAINGYAVGGGFGLSLACHIRYCVPSSKFGCSEVRWSHMAAWPSYVGQIPTGWAY
                     WFALTGQMIDADTAFRLGLVQKICAPEKLIDDCIELATTINRNGRLIAQHTVEFIEDK
                     FLYETQGWEKGFEIHREFYAHLRQSKDYDEGSAAFSQRRKPDFSQEYYDDKTRPPGVP
                     ETAK"
BASE COUNT         1079 a         1308 c         1264 g          908 t
ORIGIN      
        1 agcaaagtaa tcgaaccagc gcgattggtc ttgagaaaat gcattgtctc aacaaatgca
       61 tgcgtcagcc gctcgacgac gttacgtttg cgctggatat agccgcgggt cgtagcgaag
      121 gtccccgacg gtgagagaat cttcagttcg acggcgctgc taagcagtct gccgcctgat
      181 ttctccacat tccatgcggg cagaccggcc aacgcgccga catccagctt gcccgcgacc
      241 atcgcagcgg ccacttgggg atgcccgcca agctgcagga tcgtcacgtc cttgtccggt
      301 ttcaagccag cggtcgctag cgcattgcgt gtaaaggtgt ccgcttccga gccgtactgc
      361 gaaacgccga tggtcgcgcc tttcaaatcc tcaaggcggc gcactttcga attgcgggcg
      421 accatgagtt tttggctcga cacattggcc gacgcgccaa ggatcgcgac atccgcaccg
      481 cgcacgcgcg ctcgagtgag agcggtgacc cccgcaaacg ccacatcgac gtcaccggcg
      541 atgagcgact gaaccaatcg cgggctaccg gtgataagaa gaatctccgc aactaagtca
      601 tgcttgctga gaatcccggt ttcttttgcc atccatggca gagctagaga aacgctcggc
      661 gcgacgtacg ccattcggac tttgtccgcg gcaaagcttc gcccgccgag caactcgaaa
      721 acgagcaaaa caatcgtcca acatgctgtc ggcagccgtc tcatattttt gatttcatgg
      781 acgcacgatt atcgattcgc gccagcagcg cgccacagat cacgttccat caccggctcg
      841 atacttacta aggtcagtcg gaattgtaaa acagaaaaaa acctgccgct cgagttcgtc
      901 agtctcgttt tgaaatccaa gttcgattcc gaagtgagac cgcacgcctc tcgccttgac
      961 actatcgtca ggtccaagta agaatcgcgg acgaacgcag ccatttcccg ctctgaggaa
     1021 ttcatggaaa atgtcaccag aaccgggccg ctcaccggct atcgcgtcct cgacgtcgcc
     1081 ggtcccctcg gcttgcattg cacgaagctg ctcgcggaca tgggtgccga tgtaattaaa
     1141 atcgagtcgc cttccggtga tgagtcgcga cgcattccgc ccttcaaaga cgatattccc
     1201 catccggaaa aaagtctcta ctttcttcac ttcaacacga acaaacgaag cgtcacactc
     1261 gatatcgaga aaccggacgg ccgggctatt tttctcgagc tcgcgcgtaa ggcagacgtc
     1321 gtgttggaga ccgcgtgtcc cgggcgtatg gatgagttag gaatcggcta ttcaacattg
     1381 cgcgcgcaaa accccgggat cgtctatgcc gcgatcacgc cctttggcca gaccggaccc
     1441 tggcgcgatt acaaagccaa tgacatggct ggggttgcgc tgggtaattt gctctacctt
     1501 gcgggagaac caggcgagcc gccgctgcag ccgcccggtg aaatcgccta cggcatggct
     1561 tcaacctatg gagccttcgg cgtcgcggtc gcgctctacc atcgcctcga tagcggcaag
     1621 gggcaatata tcgatgtttc gatgcacgag tgcgccggtc acatcgccgg ctatttcatc
     1681 ccgaattacg gctacaccgg cgaaaaaccc gcgcgcgcct cgcgcaaggg ggaagagacg
     1741 gacttgtacg acccgtatcc cacaaagaac ggctacgccc gtatttttat tattcccgtc
     1801 gaacaatggc gccggctggt cgattggata ggccgcccgc ccgaaatttc cgggccggag
     1861 ttcgagaaaa tggcttaccg gcgaaagcat cccgacattg tgaccaaggc gatcgctgag
     1921 ttttgcgccc gacttactaa agaggaattg tacgatgagg gtcagaaacg gcgtatcgcg
     1981 gtgacaccga tcaataccgc gggcgaattc gtggagatgc cacagaccaa agagcgcgga
     2041 cttttcgttg aaatggagca cccggtcatc ggcaaatatc agcagttcgg cgtcgtgccc
     2101 cgcttgatgg aaactcccgg cgcgatctac cgtcccgcac cgctgctcgg cgagcataac
     2161 gaggagattt acctcgacga actcgagatg accaacgacg atctcgtcgc gttacgcgcc
     2221 gaaggcgtca tttgaaatac tggaatcatg cagtgatgaa atggtggagt gttgggaact
     2281 tcaaaaaaac cattactccc atagtccaga ggaattaaac acatgagcaa atttcccctt
     2341 gaagggattc gcgtgattga actgacgacc ggcgcggccg gcccgactgt ggcgcgagtg
     2401 ctctgcgagt tcggcgccga ggtcatccgc tgtgagactc ggctgcgcgg cgatggccac
     2461 cgcggcgagg atccaaagct ctggaacaag aaacccgatt tcatgaagct tcagcgcggc
     2521 aagaaatctt tcacggttaa tatgcaaact cccagaggcc gggagcttgt gcgcgagctg
     2581 gtgaaaaaat ccgacgtgct cgtcgagaac ttcgggctcg gcattttgga gaaatggggc
     2641 ttggactggc cgcaactgaa agaaatcaat ccgcaaatga tcctgatccg cgttaaaggc
     2701 atgggctgca cgggaccgca cgctgccgat ctcacttatg gtccgaacgt cggtaatacc
     2761 atgggcacca cctatttatg gaattatccg aacgccacca ccgccacagc cgagccgcgc
     2821 acgcagcatc ccgattttat gggtggcgtc accggcgcgt tcggtgtcgt gtgcgctttg
     2881 atccaacgga aaaaatccgg cttcggtcag tggatcgaca gcgcgcagca ggaaatcggc
     2941 gcatccttgc tgggaccgaa atacctcgac tacacggtga ataaacgcga accccggccc
     3001 gagggcaatc ggagtttgat tgctgcgcct tacggcccgt atcagtgcaa aggcgatgac
     3061 cgctggtgcg tcgttgcggt ctatagcgaa gaagagtggc aacgcttcgc cgctctgttg
     3121 gaacaatcag ggcccaaacg gagtgagaat tttgcaaccc acctgcagcg cgtgcgccac
     3181 aaggacgagt tggaccagtg ggtgacgcgt tggactctgc aatatgaacc gtacgaggtc
     3241 atggaaaagt tacaagcgat cgctatttgc gcagccgttg ttcaggacgt tgaagatcaa
     3301 ttcaagcgcg atcgacagta cgcagcgacg ggatatctgg tgcggttgaa cgaacccgaa
     3361 gccggtgaag tggtcacgga aaatgtaccc gtgcgattgt ctgaaacacc cggaaaagtc
     3421 cgcggtgtag caccgttgat gggcgagcac acccatgcca tcgcgcgcaa cctgctcggt
     3481 ctgagcgatg atgaaatcaa aaagttagac gaagagaaag tattgtatta gcagttctca
     3541 gttgccagtg attagtcgct aggaacgaat aggcgattga atttttgatc tcaactgaca
     3601 actgataact aaccactgac aactgaatca aaggaggaac catgagcgac gaaccgcaac
     3661 cgctatgtat ttatgaagaa cgcgatcccc acatcgccat tatccggctg aaccggccgg
     3721 agaaaaaaaa cgccatcagc cgcgatcttt actgggctct cgacgagtgt tggcagaaag
     3781 ccaaagccga tgacgacgtc tggagcatta ttctcactgg cagcggcgac gctttctgcg
     3841 ccggcggtga tttgaaagag aatctcgcgt tcgcgaaagg cgaaatgaaa gggccgcgct
     3901 ccgggcctcg cccttattcc aacacccgca tgctccagat gcacaagccg attatcgccg
     3961 cgatcaatgg ttacgcggtc ggcggtggtt tcggcctctc gctcgcctgc cacatccgct
     4021 attgcgtgcc gtcgtcaaag ttcggctgct ccgaagtccg ttggtcgcac atggccgctt
     4081 ggccgagcta tgtcggtcag attcccaccg gctgggctta ttggttcgcc ctcaccggtc
     4141 aaatgatcga cgctgacacc gcttttcgtt taggactcgt gcaaaaaatc tgcgctccgg
     4201 aaaagctcat cgacgactgc atcgagctgg cgacgacgat caaccgcaat ggtcggctca
     4261 tcgcccaaca caccgtcgag tttatcgaag acaagtttct ctacgaaacc cagggctggg
     4321 aaaaagggtt cgagatccac cgcgagttct acgctcattt gcgccagagc aaagactacg
     4381 atgaaggctc ggcggcgttt tcccagcggc gcaagccgga cttcagccag gagtactacg
     4441 acgataaaac ccggccgcca ggcgtgccgg aaaccgccaa ataatactag ctagtcgcct
     4501 ccacaaactc gggcgatcgg aacggtcgaa gcccttccgt cgcccgagca gtttcgatc
//
LOCUS       DASULZ010000209         3960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_227947, whole genome shotgun sequence.
ACCESSION   DASULZ010000209 DASULZ010000000
VERSION     DASULZ010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227947"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VFY96_04305"
     CDS             <1..869
                     /locus_tag="VFY96_04305"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605713.1"
                     /translation="ADAKGFFKDEGLDNETVVISGPLSIAALLSGDVDYSGAGGSGMR
                     AALKGAPIKGIMFQADKVTFYLVVDPTIKSAGDLKGKKIAIGSPGDTQDRIMTMFAER
                     AGVAARDIVRISMGADTNTRVMAVKTGAANATTVDPGGLIFAQKEGLTSLGFLGDLFP
                     MPFQGFVTIEKKLRDNPGQIKRWLKGVLRGLMFVRDRPEEAVDIGSKKLQLGNATRSM
                     MIEGVKNYLRALSPGVPGLPTHEGIKNFLEYDIRIPLQLKENVPPERLLSLQLVAEVV
                     RELEASQRRTTR"
     gene            866..1594
                     /locus_tag="VFY96_04310"
     CDS             866..1594
                     /locus_tag="VFY96_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX5605714.1"
                     /translation="MSSDKDLREQLALCTRIFAMQGMIGVFGHVSVYQPETKTVFISP
                     GMGSDKGNLRPEQMVPIDLQGKPISGTEGPPVEWPIHTALHGARSDALAIAHLHTPHA
                     TLFAIAKREFRPVTLQGAIFNEGVPLYPEAQLITNPERGNALLKAIGNKRAVLLRGHG
                     IVVVGQNLQEVLYTALVLEDDTKKTLQAATLGEVGTISDQECRTFGAEIALERRAQRA
                     WNYFASLEARWDRQPATGRSELFP"
     gene            1792..2847
                     /locus_tag="VFY96_04315"
     CDS             1792..2847
                     /locus_tag="VFY96_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605715.1"
                     /translation="MNENVRLYAGTQHGLMIWRSANGGWQEVARHFENGIIDSIQGCK
                     RSPEKVFLGVTHDGLYRTSDGGKSWGKVLDGDIRSVSVDPTDDDVIYAGVEPVGLFRS
                     EDGGESWQEITSLKTMPQSVRKNWWYPQAPHHGHVRNIHIHPDDPQTLYLCLEHGGIV
                     RSFDRGETWQDVSKGIDYLDIHVIANFPQRGDRYFVATARGFFTSDKPETGWVRAENG
                     LTRDYFHDFVFLRPKPAGELPAMLIATADGSPGFWRRENRGARAAMFKSTDGAASWTR
                     ITEGLANDLDSMIWALAHHPTDTRAVFAGFGNVARGHASGAGGAGDLMISRDLGNSWE
                     RLDIELPADRVLWAAAE"
     gene            2966..>3960
                     /locus_tag="VFY96_04320"
     CDS             2966..>3960
                     /locus_tag="VFY96_04320"
                     /inference="COORDINATES: protein motif:HMM:NF014750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5605716.1"
                     /translation="MTGSNSDYSEFFPSKRNTRLPGGYMGKILRVDMSSGNLTDLNLP
                     EEPLLRKYWGGQLFAEYVLLNELPFEIDPYDPRNIIVGMTGPVTGTGFTPGGTKMCFV
                     YLSPATRYTLGRGATSGYFGTSLKTAGYDGIIVTGAAQTPKYLYIGEDKVELRDASHV
                     WGKGIRDTEDIIRRESGHQDARIGCIGPAGENLIRAAMLVNDYNHTAAHGLGAVMGSK
                     KLKAFVAWGTKRPRLHDKLRLIDAGVRWAKAIAPRKTTVKKRLTSVGHGEDWGAITKL
                     NWRSTIITDEARGLDQNRVTLRPCFQCPRMCPWDVEIGEGRFKGKVGHFNAGSEW"
BASE COUNT          930 a         1069 c         1157 g          804 t
ORIGIN      
        1 ttgccgatgc caaaggattc ttcaaagatg aaggcctcga caacgaaacc gtcgtgattt
       61 ctggtccgct ttccatcgcg gcgttgttga gtggcgatgt cgattacagc ggcgcaggcg
      121 gcagtggcat gcgcgcggcg ctcaaaggcg cgccgatcaa aggcatcatg tttcaagccg
      181 acaaagtcac gttctacctc gtcgtcgatc caaccataaa aagcgcgggt gatctgaagg
      241 gcaaaaagat cgctatcgga agcccaggcg atacccaaga ccgtatcatg accatgttcg
      301 ccgagcgcgc cggtgtggca gcgcgggata tcgtccgaat ttccatggga gcggatacga
      361 atacccgcgt gatggccgtg aaaaccggcg cggcgaatgc gacgacggtc gatccgggcg
      421 gcttgatctt cgcgcaaaaa gaaggattga cttcccttgg cttcctcggc gatctctttc
      481 ccatgccgtt ccagggcttt gtcaccattg agaaaaaact ccgggacaat cctgggcaaa
      541 tcaaacgctg gctcaagggc gttcttcggg gattgatgtt cgtgcgcgac cgaccggagg
      601 aggcagtgga catcggcagc aaaaaactcc agctcggaaa cgccactcga tcgatgatga
      661 tcgaaggcgt aaagaattac ctacgcgcgt tgtcgcccgg cgtgcccggc ttgccgactc
      721 atgaaggtat caaaaatttt ctggaatacg acatcagaat cccgcttcag ctgaaagaaa
      781 atgttccgcc cgagcgatta ctgtcactgc agttggttgc ggaagtcgtc agagaactgg
      841 aagcatcaca aaggagaacg acaagatgag ctcagacaag gatctgcgcg agcaacttgc
      901 cctctgtact cgcattttcg cgatgcaagg aatgatcggt gtctttggcc acgtcagcgt
      961 ctaccagccc gaaacgaaaa ctgttttcat ctcgccggga atgggctccg acaagggcaa
     1021 ccttcgcccg gagcaaatgg tgccaataga cctgcaaggg aagccgattt caggcacgga
     1081 aggaccgccg gtcgaatggc caatccatac cgcactgcac ggagcgcgct ccgacgcgtt
     1141 ggcgattgcc catctgcata cgccgcatgc gactctcttc gcgattgcga aacgcgagtt
     1201 tcggcctgtc acattacaag gcgcaatttt caatgagggg gtgccccttt atcctgaagc
     1261 gcagctgatc accaatccgg aacgcggtaa tgccctgctg aaggccatcg gcaacaaacg
     1321 tgcggtcttg ttgcgcggcc atggtatcgt cgtcgtcgga caaaatttgc aggaagttct
     1381 ctatacggcc ctggttttgg aagatgatac caagaaaact ttacaggctg cgacgcttgg
     1441 ggaggtcggc acgatcagcg atcaggagtg tcgtaccttt ggcgcagaaa tcgcgcttga
     1501 acggcgggcg caacgagcat ggaactattt cgccagcctc gaagcgcgtt gggatcggca
     1561 accggccacc gggcggagcg aattgtttcc ataacgataa atagcctgca aaaagcagtt
     1621 caaacggttt cgatttttgt ttctcgggcg tcaacagcat gacacgcgga tttattctga
     1681 gggctgccga aaaagttagt gtgacacgga tacagcgatt ttgatcatca ccgaccttaa
     1741 acggagcgca gcgattgaac ggcttgaacg atctgaacgg aggccatcat tatgaacgaa
     1801 aacgtcagac tttacgctgg cacacaacat ggactgatga tttggcgctc ggcaaacggg
     1861 ggttggcagg aggttgcacg ccatttcgag aacggcatta ttgattcgat ccagggctgc
     1921 aagcggtcgc cggaaaaggt cttcctcggt gtgactcacg atggcctcta tcgtacgtcc
     1981 gatggcggca agagctgggg aaaggtgctc gacggcgata ttcgatccgt cagcgtcgac
     2041 ccaacagacg acgacgtcat ttatgcaggc gtcgagccgg tcggcctgtt tcgtagcgaa
     2101 gatggcggcg agagctggca agaaattact tcgttaaaaa ccatgccgca atcggttcgg
     2161 aagaactggt ggtatccgca agcgccgcat catggccacg ttcggaatat tcatattcac
     2221 ccggatgatc cgcagactct gtatctctgt ctcgagcacg gcggcattgt ccgaagcttc
     2281 gaccgcggcg agacctggca ggacgtcagc aaaggcatcg attacctcga tattcatgtc
     2341 attgcgaatt ttccgcaacg cggcgaccgc tacttcgtcg ccactgcgcg gggctttttc
     2401 accagcgaca aaccggaaac cggctgggtg cgtgctgaga atgggctgac gcgcgactat
     2461 tttcacgatt ttgtttttct gcgaccgaaa ccggcaggcg aactgccggc catgctgatt
     2521 gcaacggccg acggctcacc cggattctgg cgcagagaaa atcgtggcgc gcgcgctgcc
     2581 atgttcaaaa gcaccgatgg agcggcatcg tggacgcgca tcaccgaagg gctcgcgaat
     2641 gatctcgact cgatgatctg ggccttagcg catcatccca cggatactcg cgctgttttc
     2701 gccggattcg gcaacgtcgc ccgcggtcac gcctccggag ccggcggtgc cggcgatcta
     2761 atgatcagcc gcgacttggg gaatagctgg gaacggctgg atatcgaatt gcctgccgac
     2821 cgtgtgctgt gggcagcggc ggaatagcga acaggcggca attgcgttca aaccgttcaa
     2881 aaagttcaaa ttgtcggaac ctcggaacgg cttgaagggc ttgaagcatt gaagggtttg
     2941 aacgaaaaag tcatgaaggt tgtttatgac aggatccaac agcgactata gcgagttttt
     3001 tcctagcaaa cgaaatactc gccttcccgg cggttacatg ggcaagattt tgcgcgtgga
     3061 tatgagctcc ggcaacctca ccgacctgaa ccttcccgaa gaaccgctgc tacgcaaata
     3121 ttggggcggc cagcttttcg ccgaatacgt gctcctgaat gaattaccct tcgaaataga
     3181 tccgtatgat ccgcgcaata tcattgtcgg catgaccggt ccggttaccg gcacgggctt
     3241 tactcccggc ggaacgaaga tgtgcttcgt ttacttgagc ccggcgacgc gctacacgct
     3301 tggacgcggc gccacgagcg gctatttcgg tacgtcgctc aaaacggccg gctacgacgg
     3361 catcattgtc accggagctg cgcaaacgcc caaatacctc tacatcggtg aagacaaagt
     3421 cgagctgcgc gatgcgagcc acgtctgggg caaaggcatc cgagacaccg aagacataat
     3481 acgccgggaa tcaggccatc aagacgcgcg cattggctgc atcggtcccg ccggcgaaaa
     3541 tcttattcgc gcggcaatgc tggtcaacga ttacaaccac acggcggcgc acggcttggg
     3601 cgcggttatg gggtcaaaga agttgaaagc gttcgtcgcc tggggcacca agcggccgcg
     3661 tctgcacgat aagctccggc tgatcgatgc cggtgtgcgc tgggcaaaag cgatcgcacc
     3721 gcgaaaaacc acggttaaaa agcgtctgac tagtgttggc catggcgaag attggggcgc
     3781 cattaccaag ctgaactggc gcagcacaat tatcaccgac gaggcgaggg gtttggatca
     3841 aaaccgcgtg acgcttcgtc catgctttca gtgtccccgc atgtgtccct gggacgtgga
     3901 gatcggggag ggtcggttca aaggcaaagt cggtcatttc aacgccggca gcgaatggat
//
LOCUS       DASULZ010000210         7823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_735542, whole genome shotgun sequence.
ACCESSION   DASULZ010000210 DASULZ010000000
VERSION     DASULZ010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7823
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735542"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VFY96_04325"
     CDS             complement(<1..233)
                     /locus_tag="VFY96_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010295038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEX5605717.1"
                     /translation="MCGIVGYIGTREAAPLILESLRKLEYRGYDSAGIAVLNDGHVAI
                     RRCEGKLSNLETLLRRQPMTGAVGIGHTRWATHG"
     gene            complement(233..1627)
                     /gene="glmU"
                     /locus_tag="VFY96_04330"
     CDS             complement(233..1627)
                     /gene="glmU"
                     /locus_tag="VFY96_04330"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:NF010934.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX5605718.1"
                     /translation="MNQLGVILLAAGQGTRMKSAVPKVLHSLGGKALFLHVLETASRL
                     DPAVITIVVGHAARRVQDACTARDINWAIQEEQLGTGHAVLCARDKFREFEGDILILS
                     GDVPLLREQTLRMMIEHQRRSHADLSFLTAQLETPNGYGRILRDGEGAITGIIEEKDA
                     TDEQRQISEINTGLYVVSNPFLFEALAAVKNHNQQKEYYLTDIVAIALRRGKKVEAVH
                     IDDVRETLGINSREDLAKMEKNLRDKINQKWMLAGVTLQDPDTTYIEETVTIGQDTVI
                     GPNTHLKGKTVIGERCHIDGTAFLTDMEIGDEVLLKFSVVMTGSRIDRGAIVGPFAHL
                     RPGTHLGSNVHIGNFVEAKAAHVGEGTKANHLTYLGDVTIGRDTNIGAGTITCNYDGF
                     QKYKTTIGDRVQVGSDTTLVAPIAIGDDVYVATASTVRHDIPAGALVFNPREEQLREG
                     WTEQKREKMKGNKK"
     gene            2041..2769
                     /locus_tag="VFY96_04335"
     CDS             2041..2769
                     /locus_tag="VFY96_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEX5605719.1"
                     /translation="MSKTAGIVIIGNEVLSGKTRDTNSHFFCTELRQLGVELQKISTI
                     QDDIEIIGKEVAAFSKRFDYVFTSGGVGPTHDDVTIEGVAHGLGLKVVRHPDIERRMR
                     LRLGDQVNEARLRMANVPEGATLLATEAPFAAIVKIRNIYIFPGIPRILEERFHAIKE
                     TFRDTPYYLKNVYVRYGEGVIASILNDLLVKFPNLMLGSYPVLDVPDYKVKVTLESKD
                     ANYLEQALRAFIATLPADAVHRVD"
     gene            2773..4215
                     /locus_tag="VFY96_04340"
     CDS             2773..4215
                     /locus_tag="VFY96_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX5605720.1"
                     /translation="MADSSSWGVLAPYKTAPLPPLPSMLKALGPGVIFMALAQGSGEL
                     IWWPYIIAKYGLSFLFLLLPACLLQFPVTYEIGRYTVLTGESIFQGFIRLQRHFAFFL
                     WILMTLSFLWFGAFAAAGGTSLAALTNFPAGWSARGQTLFWGYASMAVFLVAILLSKV
                     IYQFVETFMWGVALLTLVGLLWASANPESLHALPAFIQGLFVPDLSMPRPWDSADATK
                     LLTAITFAGLGGFWTLFYSYWVRDKGIGMAHYMGRLTGPITGKAEAIPSSGFVPGKDE
                     GLAHVRRWRHYLFWDISIGIGGNLITTLMTCLLAYALLFPKNLLPQGYELAVVQSRFF
                     EVSWGVIGRFLFLIVAAAFLSDTWLATVDAVSRIHTDCLFAFFPKAQSISVRSWYLIF
                     LFVLTAITSATMGAAEPGPLILISAVIGFVGTVLFSAALIFLNHVYLPRHLPRLARPG
                     RLNLIFLCVSCLAYFLLAVAYLLTVTGIIQ"
     gene            complement(4254..4688)
                     /locus_tag="VFY96_04345"
     CDS             complement(4254..4688)
                     /locus_tag="VFY96_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018395353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX5605721.1"
                     /translation="MFRVTNEAIDLSELVRYVTDPEAGAIVPFIGTTRNNNEGRTVIA
                     LDYEAYPEMAEKELARIGDEAKKKWPICKMAIVHRLGPVQIGEASVMIAVSSAHRDAA
                     FAASRFAIEQIKKTVPIWKKEVFEGGEVWIGTQSGQPLTALK"
     gene            complement(4694..4939)
                     /gene="moaD"
                     /locus_tag="VFY96_04350"
     CDS             complement(4694..4939)
                     /gene="moaD"
                     /locus_tag="VFY96_04350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HEX5605722.1"
                     /translation="MTVRVKFFAILRERAGASEVSKELSEESTVGDLWRELQKDYPKL
                     AVPGIRLLYAVNQNYVGADHRLREHDEVVFIPPVSGG"
     gene            complement(4936..5442)
                     /locus_tag="VFY96_04355"
     CDS             complement(4936..5442)
                     /locus_tag="VFY96_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEX5605723.1"
                     /translation="MSVHEHKERGRKNIRFFVVTVSDTRDETTDTSGQTIRNLLQNNQ
                     HETVGYRIVKDEPEQIENLLNEALAKNDVDAVIVNGGTGIAPRDGTYEVVSRFLEKKL
                     DGFGEIFRYLSYLDIGSAAIMSRAAAGSARGKVLISLPGSEAAVKLAMEKLVLNEIRH
                     MVSQVQGK"
     gene            5518..6723
                     /gene="hemW"
                     /locus_tag="VFY96_04360"
     CDS             5518..6723
                     /gene="hemW"
                     /locus_tag="VFY96_04360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX5605724.1"
                     /translation="MPRVAGARWADYDARQMAKPFSLYIHIPYCVSKCPYCDFNSHVV
                     AEIPEQAYVDALLSELAHYGDSEEWRGRTAQSIFFGGGTPSTFKPESIGKLLAWSAAT
                     FPIEADCEITLEANPGTVDSDKFFGYHDAGINRISIGVQSFQPRLLKFLGRIHGVEEA
                     KRALEIVRQTNFETFSLDLIYANPGQTLDELAADLDSALSFQPPHLSAYNLTFEEGTP
                     FHHEYRAGKMTSLSEDEEIAMAELIETKLAAAGLLRYEISNYARPNCHSRHNSNYWRG
                     GDYLGLGAGAHSYRRNADHRTGRRWSNEKNPAKYIAQVGQTHQAVAGLEQVDRNKAAA
                     EFMFLGLRMTEGISPVAFRERFGKFPWEAFPRIKDLIEGMLLEVKHGFLRLTKKGLLV
                     ANSIFVEFM"
     gene            6736..>7823
                     /locus_tag="VFY96_04365"
     CDS             6736..>7823
                     /locus_tag="VFY96_04365"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEX5605725.1"
                     /translation="MISSDLSTLRIDHIGSLVRPAKLKEAFARHDRQQAAYEELKQAQ
                     DDAIREVIHKQEEHGLLVVTDGEFRRHNFQESFSECVTGFDVPKDVSRYYEKRNVNEN
                     PLERAEQDFDEAGPAIVTRRRALERLKLVRNLPLEEFRFAQSVATAPVKVTVLGPDRI
                     AQRFKWETSLSVYKGLDEFVDHVAQIERQIIAELVKAGCRYIQIDAPGYTAYVDKISL
                     GRMRSRGEDPERNFQRSIDADNALIEGFPGVTFGIHICRGNARTIDPATGKLVPQWHR
                     EGSYDAIAEKLFNTLKHQRILLEYDSERAGGFEPLRLVPKDKIIVLGLVSTKSSDMES
                     IEELKQRLDQASKFLSLEQLALSPQCGFG"
BASE COUNT         1639 a         2213 c         2092 g         1879 t
ORIGIN      
        1 ccgtgggtgg cccaacgagt atgaccgata cccactgcgc ccgtcatggg ctggcggcgc
       61 agcagggttt cgagattgct caatttgcct tcgcagcggc gaatcgcgac gtggccatcg
      121 ttcaaaaccg caataccagc ggagtcgtaa ccgcgatatt caagttttct caggctctcg
      181 agaatgaggg gcgctgcttc gcgcgtgccg atatagccga cgatgccgca cattatttct
      241 tgtttccttt catcttttcc cgcttctgtt ccgtccaccc ttcgcgcagt tgctcctcac
      301 gcggattgaa aaccaacgcc cccgcgggaa tgtcgtgccg caccgtgctg gcggtggcga
      361 cgtagacgtc atcgccgatt gcaatcggcg ctaccagagt ggtatcgctg ccgacctgga
      421 cccgatctcc gatggtcgtt ttatacttct gaaagccatc ataattacac gtgatggtgc
      481 cggcgccgat attcgtatcc cggccgatgg tgacatcgcc cagataggtg agatgattgg
      541 ctttggtgcc ctcgcccaca tgggctgctt ttgcttcgac gaagttgccg atgtggacat
      601 tgctccctaa atgggtgccg ggacgcaaat gcgcaaatgg cccgacgatg gcgccgcgat
      661 cgatgcggct gcccgtcatg acgacggaaa acttgagcag aacctcgtcg ccgatttcca
      721 tgtcggtcag aaaggcagta ccatcgatat gacaacgctc gccgatcacg gtcttgccct
      781 tgagatgggt gttcgggccg atcacggtgt cttgcccgat ggttacagtc tcttcaatgt
      841 aagtcgtatc gggatcctga agcgtcactc cggcgagcat ccacttttga ttaattttat
      901 cgcgcaggtt cttttccatc tttgctaggt cctctcggct attgattccc agcgtctcac
      961 ggacatcgtc gatatgcacg gcctccactt tcttccccct tcgaagcgcg atcgcgacaa
     1021 tatcggtcag ataatactcc ttctgctgat tatggttctt gaccgcggcc aacgcttcaa
     1081 acaggaacgg attcgagacg acataaaggc cggtattgat ctcggagatc tgtcgctgtt
     1141 cgtcggtcgc gtccttttct tcaatgattc cggtaatggc cccttcccca tcccgtaaaa
     1201 tccgcccgta gccattaggc gtttctaatt gggctgtgag aaaactcagg tctgcatggg
     1261 acctgcgttg gtgttcgatc atcatccgca atgtttgttc tcgaagcagt ggcacgtcgc
     1321 cactcaagat caaaatgtcg ccttcaaatt cacggaattt atctcgggcg cataagaccg
     1381 catgacccgt accaagctgc tcttcctgga tggcccagtt tatgtccctt gccgtgcagg
     1441 cgtcttgaac cctgcgcgca gcatgcccga ccacgattgt gatgaccgcc ggatcgagtc
     1501 ggctcgcggt ttccaaaaca tgaagaaaga gagcttttcc gcccagcgaa tgaagcactt
     1561 tgggaacggc agacttcatt cgggttccct gaccggcggc aagcaggatc actccgagtt
     1621 ggttcatagg tatgtcagga gatagattat agaggatgtc tgaaagcaat caacgacaga
     1681 ttgtgagccc tacaatggca attttggcca gcggccaatg caaattgccg ccgccatatg
     1741 tggcccgagg gagcgcagag aacaccggcg cagtgacctc agaaacctat ttaaacttct
     1801 gagcaggtaa gttattctgc gtccattgga gattttccta tgccggaaaa acccgctgac
     1861 gatcgtgcgc cacgcgagcc gatttcgcaa caaaactggt gggattattg tccagtctgc
     1921 agctcgaaac taatcaatga gaaatgtaag tatacttgtc cgaatccgga ttgccagttt
     1981 ttcatgagct gcagcgagtt tgatctctga gcccaccgct ttccttcgag agcaatacgc
     2041 atgtctaaga ccgctggcat cgtgatcatt ggcaatgaag ttttgtcggg caaaacgcgg
     2101 gacacaaatt cccatttttt ctgcaccgag ctgcgacaac tgggtgttga actccaaaaa
     2161 atatcgacca tccaggacga tatcgagatt atcggaaagg aagtcgcggc tttttccaag
     2221 cgtttcgatt atgtattcac cagcggcggc gtcggaccga cgcacgacga tgtcaccatc
     2281 gaaggggtcg cgcacggttt gggtctcaag gtagtccgtc atcccgatat cgaacggcgc
     2341 atgcgcttgc ggctaggcga tcaggtgaac gaggcacggc tgcgcatggc caatgttccc
     2401 gaaggcgcca cattgttggc gaccgaggcg ccctttgcag cgatcgtgaa gatccgcaac
     2461 atttacatct ttccaggcat tccgagaatt ctcgaggagc gttttcacgc catcaaagag
     2521 acgtttcgcg acacgccgta ctatcttaaa aacgtctacg tccggtatgg cgaaggtgtc
     2581 atcgcctcaa ttctgaatga cctgctggta aagtttccta acctcatgct cggatcctat
     2641 cctgttctcg atgtgccgga ctataaggtc aaagtcacgc tcgaatcgaa agatgcgaat
     2701 tacctcgaac aggctctccg tgcttttatc gctacccttc cggcagacgc agtgcaccgc
     2761 gtcgattaac aaatggccga ttcttcgtcg tggggagttc tggcgcccta caaaacggcg
     2821 ccgcttccgc ctctgcccag catgctcaaa gccttgggac ccggtgtcat attcatggct
     2881 ctggcgcagg gcagcggtga gctcatctgg tggccttaca tcattgccaa gtacggcctg
     2941 agttttctct ttcttttgct tcccgcctgc ttgcttcagt ttcctgtgac ctacgaaatt
     3001 ggccgatata cagtgctcac cggcgaaagc atttttcagg gattcattcg gctgcagcgc
     3061 cacttcgcct tctttctctg gattttgatg accttgtcct ttctctggtt cggcgccttc
     3121 gcagccgctg gcgggacttc gctagccgcg ctgacgaatt ttcccgccgg ctggtcggcg
     3181 cgcgggcaaa cactcttttg gggctatgcc tcgatggcgg tatttctcgt cgcgattttg
     3241 ctcagcaagg ttatttacca gttcgtcgaa accttcatgt ggggcgtggc tctgctcaca
     3301 ttggttggcc tgctctgggc tagcgccaat ccagagtcgc tccatgcctt gccagctttc
     3361 attcaaggcc tgtttgtgcc ggatctgtcg atgccacgcc cgtgggattc ggcggatgca
     3421 accaaattgc taaccgcgat tacttttgcc ggcctcggcg gcttctggac gcttttttat
     3481 tcgtattggg tccgcgacaa gggcatcggt atggcgcact acatgggcag gctgaccggg
     3541 ccgatcaccg gcaaagccga ggcgatccca agttccggtt tcgtcccggg aaaagacgag
     3601 gggctcgccc atgtgcggcg ctggcgccac tatttattct gggatatcag catcggcatc
     3661 ggtggcaatt taataaccac actgatgacc tgtctactgg cttatgcgct gctgttcccc
     3721 aaaaaccttc tgcctcaagg ttacgagttg gcagtggtac aaagccgctt ttttgaggtg
     3781 agctggggcg tgatcggcag atttttgttt ctgatcgtcg cggcggcgtt tctctcagac
     3841 acctggctcg cgacggtgga tgccgtgagt cggattcaca ccgattgcct gtttgcgttt
     3901 tttcctaaag cccagtcgat ctcggtgcgc tcgtggtatt tgatcttttt atttgttctc
     3961 actgcgatca cttcagctac gatgggagcg gccgagccgg ggccgctgat cttgatttcc
     4021 gcggtgatcg gttttgtcgg caccgtactt ttttctgcgg cgctgatctt tctcaaccac
     4081 gtctatttgc cgcgtcatct gccacggctt gcgcgtccgg gacgcttgaa tctgatcttc
     4141 ctctgcgtct cttgccttgc atatttcttg ttggccgtgg cctatcttct gaccgtcaca
     4201 ggaataattc agtaggaagt tcgcgaccgg gctgcgtgca aaaacgcgca ggtttacttc
     4261 agtgcggtca gcggctggcc gctttgcgtg ccaatccaaa cttcgccgcc ttcgaacact
     4321 tcttttttcc agatcggcac ggtctttttg atctgttcaa tggcgaagcg gctcgcggcg
     4381 aatgccgcat cgcggtgcgc cgatgagacg gcaatcatca cgctcgcttc accgatctga
     4441 accggtccga gacgatgtac gatcgccatt ttgcagatcg gccatttttt cttcgcctca
     4501 tcgccaattc gagctaactc cttctcggcc atctccggat aggcttcgta gtctagagcg
     4561 atgacggttc gaccttcgtt gttgttgcgc gtcgtaccga tgaaaggaac gatcgcaccg
     4621 gcctcgggat ccgtgacgta acggaccaac tcgctcaaat cgatggcttc gttcgtgacc
     4681 cgaaacatgt ctcttagcct ccgctcaccg gcggaatgaa caccacttcg tcgtgctcgc
     4741 gcaagcgatg atccgcgccc acatagttct gattgacggc gtacaacaga cggattcccg
     4801 gcacggcgag cttgggatag tctttttgca attctcgcca gagatcaccc accgtgctct
     4861 cctccgagag ttccttggaa acttccgaag cgccggcgcg ctcgcgcaaa atggcaaaaa
     4921 atttgactcg gacggtcatt ttccctgcac ttgagaaacc atatgtctaa tctcattcag
     4981 cacgagcttc tccatcgcca gcttcacggc cgcctcggaa cccggcagcg agatcaacac
     5041 tttgccgcgc gcgctcccgg cggccgcgcg gctcatgatc gcagccgagc cgatatcgag
     5101 ataactcaaa tagcgaaaga tttcgccgaa gccgtcgagc ttcttttcta aaaaccggct
     5161 cacgacctca taagtgccgt cgcgcggggc gatacccgtg ccgccattga cgatcacggc
     5221 gtcgacatcg ttttttgcca gtgcctcgtt gagcaaattt tcgatctgtt cgggctcatc
     5281 tttgacgatt cgatacccga cggtctcatg ttgattattc tgcaacaaat tcctgatcgt
     5341 ctggccgctc gtatccgtgg tttcgtcgcg tgtgtcgctc acggtcacga cgaaaaaacg
     5401 aatgttcttt ctacctcttt ctttgtgctc atgaaccgac ataggtcttt ctccgcagaa
     5461 actctcaggc gaatcatagc aagaccgggt agggttgcaa agctggttga aagccgcatg
     5521 cctcgagttg ccggcgcccg ttgggccgat tatgatgctc gtcaaatggc aaagccattt
     5581 tccctttaca tccatattcc ttactgcgtg agcaagtgcc cctactgcga cttcaactca
     5641 catgtcgtcg cggagattcc cgagcaggcc tatgtggatg cgctgctatc ggaattggca
     5701 cactacggcg attcggagga atggcgcggc cgtacggcgc agagcatttt ttttggcggc
     5761 ggcacaccat cgacattcaa gccggagagc atcgggaagc tcctcgcctg gagtgcggcg
     5821 acctttccca tcgaagccga ctgtgaaatc actctggaag ccaatcccgg caccgttgac
     5881 agcgataaat tctttggcta ccacgatgcc ggcatcaatc gcatcagtat cggcgtgcag
     5941 tcgtttcagc ccaggctgct taagttcctc gggcgcattc acggcgtgga agaagccaag
     6001 agagcactcg agatcgtccg gcaaacgaac ttcgagacct tcagtttgga tttgatctat
     6061 gccaaccccg gtcaaactct cgatgaattg gcagcggacc tggatagcgc attatcgttt
     6121 cagccgccgc atctctcggc ctataatttg accttcgagg aaggtacgcc ctttcatcac
     6181 gagtaccgtg ccggcaaaat gacctccctt tctgaagacg aagagatcgc gatggccgag
     6241 ctgatcgaga cgaaacttgc agcggccgga ttactgcgct acgaaatttc gaattacgcg
     6301 cggccaaatt gccattcgcg gcacaactcg aattactggc gcggcgggga ctatctggga
     6361 ctgggcgcgg gcgctcatag ctaccgtcgc aacgccgatc accggaccgg ccggcgctgg
     6421 agtaacgaaa aaaatcccgc caaatacatc gcgcaggtcg gtcaaacaca ccaagcggtc
     6481 gccgggttgg aacaggttga ccgtaataaa gctgccgctg agttcatgtt tcttggacta
     6541 cgcatgacgg aaggaatatc gccggtcgcg ttccgtgagc gatttggcaa gtttccctgg
     6601 gaagcgtttc cgcgtatcaa agatttgatc gagggaatgt tgctcgaggt aaaacatgga
     6661 ttcctcaggc tgacaaagaa gggattgctg gtcgccaatt ccatttttgt cgagtttatg
     6721 tgaaccggag gcaatatgat ttcgagcgat ctcagcacgc tgcgtatcga tcacatcggc
     6781 agcctcgtcc gaccggcaaa gctgaaagag gcattcgccc gtcacgatcg tcaacaagca
     6841 gcatatgaag agttgaaaca agctcaagac gacgcgatac gagaggtgat tcacaagcag
     6901 gaggaacacg gtttactcgt cgttaccgat ggtgagtttc ggcgccataa ctttcaagag
     6961 agcttttccg agtgcgtcac cggcttcgac gtgccgaaag atgtttcgcg ctattacgag
     7021 aaacggaacg tcaacgagaa tcccctcgaa cgtgccgagc aagacttcga tgaagccggt
     7081 ccggcgatcg tcacccggcg cagagccctc gaacggctaa aactcgtccg gaatcttccg
     7141 ctcgaagagt tccgctttgc tcaatcggtt gcgacagcgc cggtcaaggt cactgtgctc
     7201 ggaccggacc gcatcgccca acgcttcaag tgggaaacgt cgctgagcgt ctataaaggc
     7261 ttagatgagt tcgtcgacca cgtcgcccag atcgaacggc agatcatcgc cgagctggtg
     7321 aaggccggct gcaggtacat ccaaatcgac gcgccggggt acaccgctta cgtcgataaa
     7381 atttcactgg gaagaatgcg cagccggggc gaagatccgg aacggaattt ccagcgttcc
     7441 atcgacgcgg ataacgcgct tatcgagggt ttccccggcg tgaccttcgg catccacatc
     7501 tgccgtggta acgcccgcac gattgatccc gcaactggga agctcgtgcc gcagtggcac
     7561 cgcgaaggtt cctacgacgc gatcgcagaa aagctcttca acacgctcaa gcaccagcgc
     7621 atcctgctcg aatacgatag tgagcgggcg ggcggcttcg agccgctgcg gcttgtgccc
     7681 aaagacaaaa tcatcgtgct cggtttggtc tcgaccaaga gctcggacat ggagagcatc
     7741 gaggaactaa agcaacgtct tgatcaggcg agcaagtttt tgtcgctaga gcagctggcg
     7801 ctaagccctc aatgcggctt cgg
//
LOCUS       DASULZ010000211         8732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_735610, whole genome shotgun sequence.
ACCESSION   DASULZ010000211 DASULZ010000000
VERSION     DASULZ010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8732
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735610"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VFY96_04370"
     CDS             <1..309
                     /locus_tag="VFY96_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5605726.1"
                     /translation="KSFVLYMIKDPNDAQIVRSTIELAHGLGLKVIAEGVENSEVWQR
                     LRDFGCDEAQGYYMSRPYPQLKSQNGLIDSHTGRLAHSRSCRGCALADVAIHRALGSA
                     "
     gene            complement(250..2913)
                     /gene="clpB"
                     /locus_tag="VFY96_04375"
     CDS             complement(250..2913)
                     /gene="clpB"
                     /locus_tag="VFY96_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010042158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEX5605727.1"
                     /translation="MDINRFTQKAQEAISAAHSKAARYGHQQVDVEHLLASLLEQEGG
                     LAGSIFSKLSIDGGALKRRVEQELERMPRVSGGGSADQIYVTGRLNRLLTQAEDEAKK
                     LKDEYISVEHVILAMTDDSGAAGRVLKEFGVTREQLARALKEIRGNQRVTSQNPEATY
                     EALGKYGRDFTKLAAQGKLDPVIGRDEEIRRVVQVLSRRSKNNPVLIGDPGVGKTAIV
                     EGLAQRVVRGDVPESLKNKRVIALDMGALIAGAKYRGEFEERLKAVLKEVQESQGEII
                     LFIDELHTVVGAGAAEGAMDASNLLKPLLARGELHCIGATTLDEYRKHVEKDRALERR
                     FQPVLVDEPSVEDTISILRGLRERYELHHGVRIKDAALVTAAVLSHRYITDRFLPDKA
                     IDLVDEAGAKLRTEIESMPTELDEVSRRVMQLEIEREALRKESDAASRERLSKLDKEL
                     ADLKAQQQQLRARWDLEKEAIARVRGIKEQIEKTKLDMEQAERQYDLNRVAELKYGKL
                     VQLERQLADEENRQKEQGNMQLIKEEVDEDDIAAVVARWTGIPVSKLLEGEKEKLLQL
                     GEHLHKRVVGQEEAVEAVADAVIRARSGLKDPNRPIGSFIFLGPTGVGKTELARALAE
                     FLFDNENNMVRIDMSEYQERHTVARLIGAPPGYIGYDEGGQLTEAVRRRPYSVILFDE
                     IEKAHTDVFNVLLQILDDGRLTDGHGRTVDFKNTVVIMTSNVGSHLILAYRGGDDPES
                     FQQMKNDVLDALRQQFRPEFLNRVDEIVVFHSLSREHLKEIVEIQLQRLRARLSERHI
                     TLELNDRAREHLAAVGYDPSYGARPLKRAIQRELETPLGRSLLKGDIKDGERVAVDYD
                     REKNQLTFSARPSSGSEGAVNGDVRKSASAA"
     gene            complement(3254..3742)
                     /locus_tag="VFY96_04380"
     CDS             complement(3254..3742)
                     /locus_tag="VFY96_04380"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX5605728.1"
                     /translation="MAATDQEKNRASGNNEAQIDSLREELQREHESYLRVLADFDNYR
                     RRTERERASAARSGKREIILALLDVLDGYDRALQHVEDAPASISEGLEALHRKFVGLL
                     QAQGVTPMLTVGENFDPQFHDAIGLVESEEVQPGRVAEEVQRGYRWGDEVLRPARVRV
                     AQ"
     gene            complement(3775..5667)
                     /gene="dnaK"
                     /locus_tag="VFY96_04385"
     CDS             complement(3775..5667)
                     /gene="dnaK"
                     /locus_tag="VFY96_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX5605729.1"
                     /translation="MAKSVGIDLGTTNSVVAVMEGGQPTVIINSEGSRLTPSVVAFTK
                     TGERLVGQIAKRQSVLNAENTIYSAKRFIGRRYSEVQAEIKNVPYQVVSGPNDAVRFE
                     IAGKQYAPEEISAQVLRKLVEDASKYLGEKVSDAVITVPAYFNDAQRQATKDAGRISG
                     LNVLRIINEPTAAALAYGLDKKKNETILVFDLGGGTFDVSILDVGDGVFEVRATSGDT
                     HLGGDDFDKRIVDWVAQEFQRDQGIDLRKDRQALQRLIEAAEKAKIELSSAVETTISL
                     PFVTADATGPKHLEMKLTRAKFEQLTADLVERCIGPVKQAVSDAKVSERDIDEVILVG
                     GATRMPAVQALVRRLTGGKEPNQSVNPDEVVAVGAAIQAGVLAGEVKNVLLLDVTPLS
                     LGVETLGGVMTKLIERNTTVPTRKSETFSTAEDNQSAVEIHVLQGERELARDNRTLGH
                     FRLEGIRPAPRGLPQVEVTYDIDANGILTVTAKDKESGKEQKITISGSTQLSKEEIDR
                     MVADAQSHSEEDRRRREEVEARNNADSMAYQVERQLREMGDRVPLNEKARAEQLIAEI
                     RELVKNNASDLAKLRQLTGDLQQVAYGLSASSQQTSQGERPGGTSGSQQGGGDDDVID
                     AEFKES"
     gene            complement(6167..6391)
                     /locus_tag="VFY96_04390"
     CDS             complement(6167..6391)
                     /locus_tag="VFY96_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605730.1"
                     /translation="MSIFTMLMTIPQVLSIWIGHQAGGVSLLTWGTYFVSAVLWLWFG
                     IEKGDKNIYLPCIGWILLDSAVIVGVIIYG"
     gene            complement(6645..>8732)
                     /locus_tag="VFY96_04395"
     CDS             complement(6645..>8732)
                     /locus_tag="VFY96_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEX5605731.1"
                     /translation="FKQFSFPGGIPSHAAPETPGSINEGGELGYSLSHAYGAAFDNPD
                     LIVACVIGDGEAETGALATSWHSNKFLDPVSNGAVLPILHLNGYKIANPTVLARITHE
                     ELEQLLRGYGYTPYFVEGHEPEKMHQLMAATLDRAIDEIKRIQSNARQSKNEVHRPRW
                     PMIVLRSPKGWTGPKDVDGKRTEDYWRSHQVPMAEMHSNPEHVKLLERWMKSYKPEEL
                     FDGRGRLRAELAALPPKGARRMSANPHANGGLLLKDLRLPDFREYAVEVPSPGEVNAE
                     ATRVMGEFLRDVMKLNLENKNFRLFSPDENNSNRWQDVLEVTKRAWVAERFDYDDKLA
                     PDGRVMEMLSEHQCEGWLEGYLLTGRHGFFSCYEAFIHIVDSMFNQHAKWLEACNDIP
                     WRRPIASLNYLLSSHVWRQDHNGFSHQDPGFIDVVINKKAQVVRVYLPPDANCLLSVT
                     DHCLRTRNYVNVIIAGKQPAPQWLNMDQAVKHCTAGIGIWEWASNDRGSEPDIVMACC
                     GDVPTLETLAAVDLMRRHLPELKIRVVNVVDLMSLQSPGEHPHGLSDKDFDALFTKDK
                     PIIFAFHGYPWLIHRLTYRRTNHDNLHVRGYKEEGTTTTPFDMCVLNEIDRYHLVQDV
                     IDRLPQLGARAAYVKQAIRDALIDHKEYIYEHGEDSPEISGWTWGHKKKSGHLDSTES
                     DNIDSGGEAPIRN"
BASE COUNT         1684 a         2599 c         2395 g         2054 t
ORIGIN      
        1 aaatcgtttg tcctgtatat gatcaaagat cccaacgatg cgcagatcgt ccgctcgaca
       61 attgagctgg cgcacggcct gggactaaaa gttatcgcgg aaggtgtgga gaatagcgaa
      121 gtttggcagc gcttgaggga tttcggctgc gacgaagctc aaggttatta catgagccgc
      181 ccttacccgc agctgaaatc acaaaatggg ctgattgatt cgcataccgg ccgactcgcc
      241 cattctaggt catgccgcgg atgcgctctt gcggacgtcg ccattcaccg cgccctcgga
      301 tccgcttgac gggcgtgcgc tgaatgtcag ttggttcttt tctcgatcgt agtccacggc
      361 gactctctcg ccgtctttaa tatcgccttt cagcaaagaa cgtcccaacg gcgtttcgag
      421 ctctcgctga atcgctcttt tcagcggccg agctccatag ctgggatcat aaccgacggc
      481 ggcgagatgc tcgcgggccc ggtcgttcaa ctcaagcgtg atgtgacgct cggacagacg
      541 agcccgcaag cgttgaagct gaatctccac gatttccttt aggtgctcgc gcgagagact
      601 gtggaacacc acgatttcat cgacacggtt gagaaactcg ggccggaact gctggcgcag
      661 cgcgtcgagg acatcatttt tcatttgttg aaacgattcc ggatcatctc cgccgcgata
      721 ggcgagaatc agatgactgc cgacattcga ggtcatgatc acgacggtat ttttgaaatc
      781 taccgttcgg ccatggccat cggtcagacg gccgtcatcg agaatctgca gcaagacatt
      841 gaacacgtcc gtatgcgctt tctcgatttc atcgaagaga atcacagagt acgggcgccg
      901 ccgcacagcc tcggtgagct gcccgccttc atcgtagccg atgtaccccg gaggcgcgcc
      961 gatcagccgc gctacggtat gcctctcctg atactcggac atgtcaatgc gcaccatatt
     1021 gttctcgtta tcgaataaaa actcggccag agcgcgcgcc aattccgtct tgccgacgcc
     1081 agtgggcccg aggaaaataa atgacccgat cggccggttg ggatccttca agcccgagcg
     1141 ggcccggatc accgcatccg ccaccgcttc gacggcttct tcctggccga cgacgcgctt
     1201 gtgcagatgc tcgccgagct gtagcagttt ctctttttcg ccttcgagca gtttcgaaac
     1261 cggaataccc gtccatcgcg ccacgacagc ggcaatatcg tcctcatcga cttcctcttt
     1321 gatcaactgc atgttgccct gttccttttg cctgttttcc tcgtccgcca gttggcgctc
     1381 gagctggacg agcttgccgt atttgagctc cgcgacgcgg ttcaaatcgt actggcgctc
     1441 tgcttgttcc atgtccaact tggttttttc gatctgctct ttgatcccgc gcacccgagc
     1501 aatggcttcc ttctccaggt cccaacgcgc ccgcagttgc tgctgctgcg ctttcaaatc
     1561 cgccagctcc ttatcgagtt tgctcagccg ctcgcgcgag gccgcatcgc tttctttacg
     1621 cagcgcttcg cgttcgatct cgagctgcat gacgcggcgc gaaacttcgt cgagctcggt
     1681 cggcatcgat tcgatttcgg tgcggagctt ggcgcccgct tcatcgacaa gatcgatcgc
     1741 cttgtcggga aggaagcggt cggtgatata gcgatgcgag aggactgcgg cggtcacgag
     1801 cgccgcgtct ttaatgcgca cgccgtgatg gagctcataa cgctcgcgca agccgcgcaa
     1861 aatcgagatc gtgtcttcca cgctcggctc atcgaccaaa acgggttgaa agcggcgctc
     1921 cagcgcgcgg tctttctcaa catgttttcg gtactcgtcc aaggtcgtcg cgccgatgca
     1981 atgcagctcg ccgcgagcga gcaagggttt cagcaggtta gacgcgtcca tcgcgccttc
     2041 cgccgccccc gctccgacca cggtgtgcag ctcatcgatg aacagaataa tttcaccctg
     2101 cgattcctgc acctccttga gcacggcctt gagccgctct tcaaattcgc cgcggtattt
     2161 ggctccggcg atgagggcgc ccatgtccag tgcgattact cgcttgtttt tgagactctc
     2221 cggcacatcg ccgcgcacga cgcgttgcgc caagccttcg acaatggcag ttttgccgac
     2281 cccaggatcg ccgatcagca cggggttatt tttgctccgg cgcgagagca cctgcacgac
     2341 tcgccggatt tcttcatctc ggccgatgac cggatcgagc ttgccctgag cggcgagctt
     2401 ggtgaaatcg cggccgtatt ttcccagcgc ttcgtatgtc gcttcgggat tctgcgaggt
     2461 gacgcgttga ttgccgcgaa tttctttgag cgcgcgcgct agctgttcgc gtgtgacgcc
     2521 aaattcttta agaacgcgtc cggcggctcc cgaatcgtcg gtcatcgcca gaatgacgtg
     2581 ttctacggag atatattcgt ctttgagctt tttggcttcg tcttcggcct gcgtgagcaa
     2641 acggtttaac cggccggtga cataaatctg atcggcgctg ccgccgccgg acactcgcgg
     2701 catccgctcc agttcctgct ccacgcgccg ttttagcgcc cctccatcga tggataattt
     2761 gctaaagata gagcctgcca ggccgccttc ctgctcgagc agagacgcga gcagatgctc
     2821 cacgtcgacc tgctgatggc cgtagcgcgc agccttgctg tgcgcggcgc taattgcttc
     2881 ctgggctttt tgggtaaagc gattgatgtc catgaatcct ccatggtggt ggtttcaaga
     2941 aagagaacac tgatgtccta ttttgcataa tgtgctgcgt agccactcat tctcccgctg
     3001 gagcacgcaa tatttgtcgc gcaggtcgag aacgaccgcc acccccgcga gattgatacc
     3061 gagagattct cgttataaac gaatcgtgcc cggccgccaa actgcgcggt ttcagaactc
     3121 cggagaactc gtcataatct cggaataaac tgccatgctt ttgcagtccg aaattccccc
     3181 tgcgggtccg ttgtatattc gcaatcagcg tcgatcgagg aaagccagag gcaagactca
     3241 aaacgcgcag tgtttattgc gcgacacgca cgcgcgccgg cctgagcact tcgtcgcccc
     3301 agcgatagcc gcgctgcacc tcttccgcca ctctgcccgg ctgaacctcc tcgctttcta
     3361 ccaacccgat cgcgtcgtgg aattgcggat cgaagttctc accgaccgtc agcatcggcg
     3421 tcacaccctg tgcctgcagt aaaccaacaa actttctatg caacgcctcc agaccttcgg
     3481 atatcgatgc aggcgcatcc tccacgtgct gcagcgcgcg gtcatagcca tcgagcacgt
     3541 ccagcagggc aagaattatt tctcgcttgc cgcttcgagc ggcgctggcg cgctcgcgtt
     3601 cggtgcggcg gcgataatta tcaaaatcgg cgagcacgcg cagatagctt tcgtgttccc
     3661 gctgcagctc ttctctaagg ctgtcgatct gtgcctcgtt atttccgcta gcgcgattct
     3721 tttcctgatc tgtcgctgcc attttccacc tattgaaacg acttttccgc gcgtctagct
     3781 ctctttgaac tccgcatcga tgacatcatc atctccgccg ccctgttgag aaccgctcgt
     3841 tcctcccggt cgctcacctt gcgaggtctg ttggctggac gcggataatc catacgcgac
     3901 ttgctgaaga tcgccggtaa gttggcgcag cttggcaagg tcggatgcat tattcttcac
     3961 cagctcacga atctcggcga tcagttgctc ggcgcgggct ttctcgttca gcggtacgcg
     4021 gtcacccatt tcgcgcagct gccgctcgac ctgatacgcc atggaatcgg cgttgttacg
     4081 cgcttccacc tcttcacggc ggcggcgatc ttcttcagaa tgggactgtg catcggcgac
     4141 cattcgatcg atctcttctt tagagagctg tgtcgagccg gaaatggtga ttttctgctc
     4201 cttgccggat tctttgtctt ttgccgtgac ggtcagaatg ccgttcgcgt cgatatcgta
     4261 agtgacttca acctgcggaa gcccacgcgg ggccggccgt atgccttcga gccggaagtg
     4321 gcccaaggtg cggttatcgc gggccagttc gcgctcgccc tgcagcacat ggatctccac
     4381 cgccgactga ttatcttccg cggtcgaaaa agtttcagat ttccgcgtcg ggacggttgt
     4441 gttgcgctcg atcagtttgg tcatgactcc acccagtgtt tcgacaccga gcgacagcgg
     4501 ggtaacgtcg agaagaagaa cgttcttcac ctctccggct agcacacctg cctggatcgc
     4561 tgcgccgaca gcgacgactt cgtccgggtt gaccgactga ttcggttcct tgccaccggt
     4621 gagccggcgc actagcgctt gcactgcggg catgcgggtt gcgccgccaa cgaggataac
     4681 ctcatcaatg tcgcgctcac tgaccttcgc atcggaaacc gcctgcttta cggggccgat
     4741 gcaacgttca acaagatccg ccgtaagctg ctcgaatttt gcgcgcgtga gcttcatctc
     4801 gagatgtttc ggcccggtgg cgtccgccgt gacaaacggc aggctgatcg ttgtttccac
     4861 ggcgctggaa agctcgattt tcgccttctc cgcagcttcg atcagccgct gaagtgcctg
     4921 gcgatctttt cggagatcga tcccctgatc gcgctgaaac tcctgcgcca cccagtcaac
     4981 gatccgctta tcgaaatcat cgccgcccag atgggtatcg ccggaggtgg cccggacttc
     5041 gaagaccccg tcgccgacgt cgagtatcga aacatcgaaa gttccgccgc cgagatcgaa
     5101 gaccagaatc gtttcgttct ttttcttatc gagcccatag gccagcgcgg ctgcggtagg
     5161 ctcgttgatg atgcggagca cattgagacc ggagatcctg ccggcatctt tggttgcctg
     5221 gcgctgcgca tcgttgaagt aagcgggaac ggtgattaca gcatcgctaa ccttctcgcc
     5281 taaatacttc gatgcatcct cgacgagttt gcgcagcacc tgcgccgaaa tctcctccgg
     5341 cgcatattgc ttgcccgcga tctcaaagcg caccgcgtcg tttggccccg agactacttg
     5401 gtagggaacg ttcttgattt ccgcctgcac ttcggagtac cggcggccga taaaacgttt
     5461 ggccgagtag atggtatttt ctgcattaag aactgactgg cgtttggcga tttggccgac
     5521 caggcgttcg cccgtttttg taaaggcgac cactgacggc gtcagtcggc tcccctcgct
     5581 attgatgatg acggtgggct gcccgccttc cataactgcg acgacagaat tggtcgtgcc
     5641 cagatctatt cccactgatt tcgccatagc agctcctcct tgcttctcgt cgtcaaatcg
     5701 ccgattcgga cagacccggc aacgtacgat ttagcctttg attaattttt ctgaatttac
     5761 gattgctcga attaggctta gctcctgtcc cgtttcgaga gagcctggcg agacttacgc
     5821 tgcaagcact ctccttccgt actcggagac gagactgttc aacgctgtga cgatgcatct
     5881 gagcgtttag gccgtgcgag gctgctctat taagacgcta acaccgcgac ggtgtcagtc
     5941 aatgggttct ttgcagatca aaccaagata acgggctgaa ggtcagttct gcacgaactg
     6001 cttgctcgtt atggtgacga tttcactgcg cggtaaagac aatcgatcac tgcatcgcca
     6061 ccgaaagatc gagaaatgcg taattggact gcgcacgccg cgaaccgaag ggacgccatt
     6121 cgatcgtgcc gcggcgggcc gcgtacaaac gacgcgcgaa agcgagttag ccgtagataa
     6181 taactcccac gatcacggcg ctatcgagca ggatccagcc gatgcagggc agataaatat
     6241 tcttgtcgcc cttctctatt ccaaaccaga gccaaaggac ggccgaaaca aagtaggtgc
     6301 cccaggttaa taacgacact ccgcccgctt gatgaccgat ccagatggaa agtacctgcg
     6361 gtatggtcat taacatggtg aaaatagaca tgcctcccaa gatgcggcgc agccgtgtgt
     6421 cgggggctag agggcacagg atggacattg gggtcttgct gcatggcgct tatcctgaag
     6481 attttcagac cgaaacgcgc tgcattaggc tgtttccagg cggccgacga tcgaggcggt
     6541 gtggggtttt ctttgaggag gaaccggcaa ctcgtgcctt tcccctcggc atggcgatct
     6601 tcggtcaatc tttgtgttcg gagtttacgc cagttagcac atggctaatt tctaataggc
     6661 gcttcgccgc cgctatcaat attatcgctt tcggtggaat ccagatggcc ggatttcttt
     6721 ttatgacccc atgtccaacc gctaatctcc ggcgaatcct cgccgtgttc gtaaatgtac
     6781 tctttgtgat cgatgagcgc gtcgcggatt gcctgcttga cataagcggc ccgcgcgccc
     6841 aactgcggca agcgatcgat cacgtcctgc acgagatgat agcggtctat ttcattcagc
     6901 acgcacatat cgaagggcgt ggtcgtcgtc ccttcttcct tgtagccgcg cacgtggaga
     6961 ttgtcatggt tggtacggcg gtaggtgaga cggtgaatca gccacggata accgtgaaaa
     7021 gcgaaaataa tcggcttgtc tttcgtgaag agcgcgtcga aatctttgtc gctcaaaccg
     7081 tgcgggtgct cgccgggcga ttgcaggctc atcaggtcga cgacattcac gacacggatt
     7141 tttagctcgg gcagatggcg gcgcatcagg tccacggcgg cgagcgtttc cagcgtcggg
     7201 acgtcgccgc agcacgccat cacgatgtcc ggctcgctgc cgcggtcatt actcgcccat
     7261 tcccagatgc cgatccccgc cgtgcagtgc ttgaccgcct ggtccatgtt gagccactgc
     7321 ggagcaggct gttttccggc aatgatgacg ttgacgtaat tgcgggtccg taaacagtga
     7381 tccgtgacgg agagcaggca attcgcatcc ggcggcagat acacacggac aacttgcgct
     7441 tttttgttga tcaccacatc gataaagcct ggatcctgat ggctgaatcc gttgtggtcc
     7501 tgccgccaca cgtgcgaaga cagcaaataa ttaagcgatg cgatgggcct gcgccacggg
     7561 atgtcattgc acgcttccaa ccatttggcg tgctggttga acatcgaatc gacgatgtga
     7621 atgaaagctt cgtagcacga gaaaaagccg tgtcgtccgg ttagtagata accttcgagc
     7681 caaccctcgc attgatgctc gctcagcatc tccatgacgc ggccatccgg cgccagcttg
     7741 tcatcgtagt cgaagcgctc tgcgacccag gcgcgctttg tgacttcgag cacgtcctgc
     7801 cagcggttcg aattgttctc gtcggggctg aacaggcgaa aatttttatt ctctagattg
     7861 agcttcataa catcgcgtag gaattctccc atcacccgcg tcgcctcggc attaacttca
     7921 cccgggctcg gcacctccac cgcatactcg cgaaagtccg gcaggcggag gtctttcagc
     7981 aggagacccc cattggcgtg cgggtttgcg ctcatccgcc gcgctccttt cggaggaagc
     8041 gcggccaact cggcgcgcag ccggcctctt ccgtcgaaca gctcttcggg cttgtagctc
     8101 ttcatccagc gctcgagaag tttcacgtgc tccggattac tgtgcatttc ggccatgggc
     8161 acctggtgag agcgccagta gtcttcggtt cgcttgccat caacgtcctt gggccccgtc
     8221 cagcctttgg ggctgcgcaa cacaatcatc ggccagcgcg gccggtgcac ttcatttttg
     8281 gactgccttg cattgctttg aatccgcttg atctcatcga ttgcgcggtc gagcgtggcc
     8341 gccatcagtt gatgcatttt ttccggttca tggccctcga cgaaataggg cgtgtagccg
     8401 taaccgcgca ataattgctc gagctcttcg tgtgtaatgc gtgccaacac ggtgggattg
     8461 gcgatcttgt aaccattgag atgcaggatc ggcagtactg cgccgttgct cacgggatct
     8521 aagaacttgt ttgaatgcca gctcgtcgcc aacgctccgg tttccgcttc gccgtcaccg
     8581 atgacgcacg caacgatcag atcggggtta tcgaacgccg cgccgtacgc gtgcgacagc
     8641 gaataaccca gttcgcctcc ctcgttgatg gaaccgggag tttcgggcgc cgcgtggctc
     8701 gggatgccgc cgggaaacga gaactgcttg aa
//
LOCUS       DASULZ010000212         2063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_26374, whole genome shotgun sequence.
ACCESSION   DASULZ010000212 DASULZ010000000
VERSION     DASULZ010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26374"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="VFY96_04400"
     CDS             complement(<1..987)
                     /locus_tag="VFY96_04400"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5605732.1"
                     /translation="MAQRAVEIHESLKHPVIDGDGHWIEPIPVFLEYLAEVGGAKSVD
                     QMRALWHSRDAWYRATPEERQHHRLRRAIWWGVTSNTLDKATGLLPALLNERLPELGI
                     DFAMIYPSFGLSINGISQYELHRAACRAYNMMTADMFAPYASRFAPAAIIPTQTPDEA
                     LEELDYAVAQRGFKVIMMRGNQERAIPSAAEGIDRQKAAWYIDTIALDSPHNYEPVWK
                     RCVELGVAVTQHSGSGRWMDRSSINNFTYNHVGHFAESNHAFARGVFLGGLVRRYPTL
                     NFGFMEGGISWACQMCLDLIEHWEKRRRDGLQYPDSISVAELRGFIERYGDQK"
     gene            complement(1124..1717)
                     /locus_tag="VFY96_04405"
     CDS             complement(1124..1717)
                     /locus_tag="VFY96_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011767469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEX5605733.1"
                     /translation="MKQVEFWFDFASSYSYPAALRIEEVARAQSVPIIWRPFLLGPIF
                     KAQGWHDSPFNIYPAKGNYMWRDLERLCESLRIPFRKPSIFPRNGVLAASIACLFSEE
                     PWIPQFVRHMFLANFAEDRDISDISVLASALERVGQPASLHACARLAEGKAKLRSATE
                     RSLAIGIFGAPSFVVGSELFWGNERLDTALAWARRSA"
     gene            complement(1812..>2063)
                     /locus_tag="VFY96_04410"
     CDS             complement(1812..>2063)
                     /locus_tag="VFY96_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605734.1"
                     /translation="GGHKDDKSLQELALPERFKWVPESIRNGPEYDVGQMWVKFAEAI
                     RAGGKTDPDFPDFDHAVRRHCTLDAIVRASESGQRQRFA"
BASE COUNT          451 a          619 c          563 g          430 t
ORIGIN      
        1 cttttgatcg ccgtagcgct cgataaaccc acgcagctcg gcaacgctga tggaatccgg
       61 gtactgtaaa ccgtcgcggc ggcgcttctc ccagtgttcg atcaggtcga ggcacatctg
      121 gcaggcccag ctaattccgc cttccataaa gccgaaattg agagttggat aacggcgcac
      181 caggccgccg agaaacacgc cgcgcgcgaa agcatggttc gattcggcga agtggccgac
      241 atgattgtag gtaaaattgt tgatcgacga gcggtccatc cagcgtccgc tgcctgaatg
      301 ttgcgtgacc gcgacgccga gctcgacgca ccgcttccag accggttcgt agttgtgcgg
      361 gctgtcgaga gcgatcgtat cgatatacca agcggccttt tgccgatcga tcccctcggc
      421 tgcgcttgga atcgcccgct cctgattgcc gcgcatcata atcactttga aacctcgctg
      481 cgccaccgca taatccaatt cctcgagagc ttcgtccggc gtctgggtcg gaatgatcgc
      541 agccggcgca aacctcgagg cgtatggcgc aaacatatcc gccgtcatca tgttgtaagc
      601 gcggcacgcc gcgcggtgca attcgtattg cgagatgccg ttgatgctga gtccgaaact
      661 gggatagatc atcgcgaagt caataccgag ctccggtaag cgctcgttaa gcaacgccgg
      721 caacagccct gtcgccttgt ccagcgtgtt gctcgtaacg ccccaccaga tcgcgcggcg
      781 cagccgatga tgctggcgct cctctggcgt agcgcgatac caggcgtccc ttgagtgcca
      841 gagcgctctc atttgatcga cggattttgc gccacccact tcagcgaggt attcgagaaa
      901 gaccggaata ggctcgatcc aatggccgtc gccatcgatc acgggatgct taaggctttc
      961 gtgtatctct accgcgcgct gtgccatgtg atcattctcc tttcgcgaat tttactttcc
     1021 ggtagcgccg cggcaacggc gcaaaaatct acgcgatcca aaaactatcg ctaagccgcc
     1081 gaatcgtcaa tcgcaacgtg acacgcgatg ggatcagccg gaatcaggct gagcggcgcg
     1141 cccatgcaag cgcggtgtcg agccgctcgt tgccccaaaa tagctcggaa ccgacgacaa
     1201 agctgggcgc gccgaaaatt ccaatggcta aagagcgttc ggttgcgctt cgaagttttg
     1261 cctttccttc agccaatcga gcacaagcgt gcaaactcgc cggctgtccc actcgttcga
     1321 gagcggacgc aagcaccgaa atgtccgaga tatctcggtc ttctgcgaag ttcgcaagga
     1381 acatatggcg cacaaattgt ggaatccaag gctcttcgga aaataggcac gctatgctcg
     1441 ccgccagaac accgttacgc ggaaagatcg acggctttcg aaacggtatg cgaagcgatt
     1501 cgcataagcg ttccagatcg cgccacatgt agttcccttt ggcgggataa atattgaatg
     1561 gagaatcgtg ccaaccctgc gctttgaaaa tcggaccgag caagaacggc cgccaaatga
     1621 tcgggacgct ctgagcccgc gcgacctctt cgattcgaag cgccgccgga taggagtagc
     1681 tgctcgcgaa atcaaaccag aactcaactt gcttcatgtg acgtatgaaa aatcgcgctc
     1741 tatagctcac ctgccggccg gtgagcgaag aactgaccta cgtcgcactt gcaacgcggc
     1801 atgaaccggc cttaggcgaa cctttgccgt tgcccgcttt ctgaggcgcg cacaatcgca
     1861 tccaaggtgc agtggcgccg caccgcatga tcgaaatcgg ggaagtcggg atccgttttc
     1921 ccgcctgcgc gtatcgcttc ggcaaatttg acccacattt gaccgacatc gtattcagga
     1981 ccgttacgta tgctttccgg cacccacttg aatcgctccg gtagcgccag ctcttgcagt
     2041 gatttatcgt ccttgtgtcc ccc
//
LOCUS       DASULZ010000213         2982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1345525, whole genome shotgun sequence.
ACCESSION   DASULZ010000213 DASULZ010000000
VERSION     DASULZ010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345525"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1402)
                     /locus_tag="VFY96_04415"
     CDS             complement(173..1402)
                     /locus_tag="VFY96_04415"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005373395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEX5605735.1"
                     /translation="MAELNEQIEGNAENLVKTRKSRSGFWFGLILILIIFAVAGAGYY
                     FLMQLRDRQSNLGGEVQKEMAKKISDYQAQLTAIQEQLSTLESTVSNKDDHFTKRLDD
                     VSRQQSERLDDARKELNEEITRLQRQLGKTRGDLLIADAEYLLGIANERLQLVGDVNT
                     AREALEAADERLHESGDAAVFKVREEIAKEIAALKNVEAADIVGLYASLRSLQDTSEN
                     LTLLLPYAGKTLTPSTEIHSHADKKEPDHELLDSAIESLQGVVTIRHSDQPIKATLTP
                     EEAQFIREQLRAKLETVKIALVQQNEALYRAGLQDAKKWAELNFTQNPETRKFIAELD
                     RFLAINIRSHFPDISASLKLLRDITKLRIETDKALQSAPAEKPKTPEQKALPEAPATV
                     TPPPAPAAPAQPEPTKK"
     gene            complement(1386..2174)
                     /locus_tag="VFY96_04420"
     CDS             complement(1386..2174)
                     /locus_tag="VFY96_04420"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005373394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX5605736.1"
                     /translation="MSSLSGAHILVTRPRHQASNLCRLIEQQHGVAVAFPTLEIIAAN
                     DLSQISGTLAKLYRFQWVVFISANAVNFALLANDGKIDQFRNVRVAAIGQATATALAA
                     AGLTADLFPAHGSDSRALLAMPAMRQVLGQRILIVRGAGGLDTLRQELQNRGADVDYL
                     DVYQRTIPLADPAPVLDLLKRNRLDLIIATSGEAVKNLLIMLGGKDQNLALTIPLVVV
                     SERIRQIAENSGFERIFVTDSPSDTAILKKIITWATGEKSGRTE"
     gene            complement(2171..>2982)
                     /gene="hemC"
                     /locus_tag="VFY96_04425"
     CDS             complement(2171..>2982)
                     /gene="hemC"
                     /locus_tag="VFY96_04425"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX5605737.1"
                     /translation="MTTKGDKILDAPLAKVGGKGLFVKELEQGMLDGEADIAVHSMKD
                     VPVEFPDGLHLAAILKREDPTDALVSNRFSRLDELPVKARIGTSSLRRQCQIKQRFPD
                     AEILPLRGNVNTRLAKLDAGDFDAIILASAGLIRLGMEERIKERLDPLISLPAIGQGA
                     IGIECRTDDPEINALLNVLHDDDTALCVSAERAMNARLQGGCQVPIAGFAEKHGERLF
                     MRGLVGAPDGSKIYRSESWNDLGQAENLGRHIADDLLAQGADLILAALYQE"
BASE COUNT          590 a          829 c          817 g          746 t
ORIGIN      
        1 aaaaacgtaa tgatcaacgc gaccgcgaaa gcggcgagcg ttgtctcgat cgaccagtgg
       61 ccgatgccca ccaacacata gccaggggtt tccaggctct tgaaaccgta ataaaccgca
      121 agtgcggctg caatcgtaac aagcagcggc cccaaaaaat aaaggatctt tttcattttt
      181 tagtaggctc cggttgagcg ggtgcggccg gggccggcgg tggcgtgacc gtcgccgggg
      241 cttccggaag cgctttctgt tcaggcgttt tcggcttctc ggccggcgcg ctctgcaggg
      301 ctttgtccgt ttcgatccgt aatttggtaa tgtctctgag cagtttcaga gaagcgctga
      361 tgtcagggaa atggctgcgg atattaattg ccaaaaaccg gtcaagttca gcaatgaatt
      421 tacgcgtttc cggattctga gtgaagttaa gttcagccca tttttttgca tcctgcagtc
      481 cggcgcgata cagcgcttcg ttttgctgta cgagcgcaat cttgaccgtt tccagcttcg
      541 cgcgtaattg ttcgcgaatg aattgggctt cttccggcgt cagggttgcc ttgatcggct
      601 gatcggaatg ccggatcgtg acgacgcctt gcaagctttc gattgccgag tcgagcaatt
      661 catggtcggg ctcttttttg tcggcatggc tatggatttc ggtcgaaggc gtcagcgttt
      721 taccggcata gggcagcagc agagtcagat tttcggaagt gtcctgaagg cttcgcaacg
      781 aagcgtataa tccgacgatg tctgccgcct caacattttt cagtgcagca atttctttcg
      841 cgatttcctc gcgcaccttg aataccgccg catcgccgct ttcatgaagg cgctcgtcgg
      901 cggcttcaag cgcttcccgt gcggtattca catcgccgac caattgcagt ctttcattgg
      961 cgatacccaa taaatactcg gcatccgcga tcagcaagtc gccccgcgtt ttgccgagtt
     1021 ggcgctgcag gcgggtgatt tcctcgttca gttctttgcg ggcgtcatcc agtctctcgc
     1081 tttgttgcct ggagacgtcg tcgagccttt tggtaaagtg gtcgtccttg ttggataccg
     1141 tcgactcgag agtggataat tgctcctgga tcgccgtaag ctgggcctgg tagtcggaga
     1201 ttttcttggc catctctttc tggacttcgc cgcccagatt ggattggcgg tccctgagct
     1261 gcatcaggaa atagtaaccg gctccggcaa cggcgaaaat gatcaggatg aggattaagc
     1321 caaaccagaa tccgctacgg gatttccgtg tttttactaa attttccgca ttcccttcta
     1381 tttgttcatt cagttcggcc acttttttca cctgttgccc aagttattat ttttttgaga
     1441 attgctgtat ccgaagggct gtccgttaca aaaattcgtt caaatccact gttttcggcg
     1501 atttgccgga tacgctcgct gacgaccacg agaggaatcg tcagtgccag gttttgatcc
     1561 ttcccgccca gcatgatcaa taagtttttt acggcttcgc cactggtcgc gatgatcaga
     1621 tcgagccggt ttcgtttcag cagatccagc accggcgccg gatcggcgag cgggatcgtc
     1681 ctttgatata catccaggta gtcgacatcc gcaccgcgat tttgtaactc ctggcgcaat
     1741 gtgtcgagac cgcctgcacc gcgcacgatc aggattcgct ggcctaaaac ctgccgcatc
     1801 gccggcattg ccagcagcgc ccggctatcc gaaccgtgtg cgggaaaaag atcggctgtt
     1861 aagcccgcgg cggcaagcgc cgttgccgtc gcctggccga tcgcggcaac ccgcacgttg
     1921 cgaaattggt ctattttgcc atcattcgcg agcagtgcaa aatttactgc atttgcacta
     1981 atgaaaacaa cccattggaa tcgatacagc tttgccagtg ttccggagat ttgactgaga
     2041 tcgtttgcgg caatgatttc gagagtcgga aacgcgacag caacgccgtg ctgctgttcg
     2101 atcagccggc acagattgct tgcctgatgc cgcgggcgag tgaccaggat gtgcgctccg
     2161 ctcagcgaac tcattcttga tacagtgccg cgagaattag atctgcgcct tgcgccagaa
     2221 ggtcgtcggc aatgtgcctg cccaggtttt ccgcttgacc caaatcattc caactttcgc
     2281 tgcggtaaat cttcgacccg tccggcgcgc cgaccaagcc gcgcatgaac aggcgctcgc
     2341 cgtgcttttc ggcgaaccct gcgatcggca cctggcagcc gccttgcaag cgggcgttca
     2401 tcgcacgttc cgcacttacg caaagtgcgg tatcgtcgtc gtgcaaaacg ttaagcaagg
     2461 cattgatttc agggtcatca gtgcggcatt cgatgccgat cgcgccctga ccgatggcgg
     2521 gcaggctgat gagcggatcg agacgctcct ttatcctttc ttccatgccc aagcgaatca
     2581 ggcctgccga ggccaggata atcgcgtcga aatcgccggc atcgagcttg gcgagccggg
     2641 tgttcacgtt gcctcggagt ggcagaattt cggcatcggg aaagcgctgc ttgatctggc
     2701 attgccggcg caggctcgag gtgccgattc tggcttttac cggcaactcg tcgaggcggc
     2761 tgaaacggtt cgatactagc gcgtcggtcg gatcttcgcg tttaagaatg gctgccaaat
     2821 gcagcccatc cggaaactcg accggcacat ccttcatcga atgcaccgca atgtcggcct
     2881 cgccatcgag catgccttgt tcaagctcct tcacgaacaa tcccttgccg cccacctttg
     2941 cgagcggagc atccagaatc ttgtcgccct tggtggtcat tt
//
LOCUS       DASULZ010000214         2446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_330015, whole genome shotgun sequence.
ACCESSION   DASULZ010000214 DASULZ010000000
VERSION     DASULZ010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330015"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VFY96_04430"
     CDS             complement(<1..368)
                     /locus_tag="VFY96_04430"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HEX5605738.1"
                     /translation="MARTAKVERKTKETDIKLSLEIDGAGKAAIETGMPFLNHMLDSF
                     SRHGFFNIDIEAKGDLKVDYHHTVEDVGLALGQAFKDALGDKRGIRRFGEASCPLDEA
                     LAKVVVDLSGRPYLFYNVKIR"
     gene            complement(417..1712)
                     /gene="hisD"
                     /locus_tag="VFY96_04435"
     CDS             complement(417..1712)
                     /gene="hisD"
                     /locus_tag="VFY96_04435"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX5605739.1"
                     /translation="MPIRLVKTTEATFKPLLQRILERRGTRDGSVERRVAEIIEAVRR
                     QGDRAVLRYTARFDHVRLNPSTVEVKQSEIEAAMKTLPREDLAALRLAVKRISIFHRH
                     QLQKSWRYRDPLGMLLGQRITPLKRVGIYVPGGKALYPSTVLMNALPARVAGVDEIIM
                     TSPLGADGSILLAAAHLAGVNRVFRIGGAQAVAAMAFGTQTIPKVDKIVGPGNIFVAT
                     AKRMVFGEVNIDSIAGPSEILVLVDNSADPVHVAADMLSQAEHDELAAAICVTTSSSS
                     AQRIQTAIEDRLCRTKRQAITLQSLKKYGAIIVASGIEEMIELANDIAPEHIELIVKE
                     PEKLAARIRNAGAIFLGPYSAPPLGDYLAGPNHVLPTGGSARFFSPLGTYDFLKRTTI
                     IRAERRALKALAPTITHLARLEGLDDHARSVEARFRQDV"
     gene            complement(1771..>2446)
                     /locus_tag="VFY96_04440"
     CDS             complement(1771..>2446)
                     /locus_tag="VFY96_04440"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003622237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX5605740.1"
                     /translation="LARALNAMGARIEGAGGAIVQIDGVKALHGVRHRIIPDRIEAGS
                     FIIAAALAGGDVLIKGARAEHLDAFLIKLKEAGIHFVSDLAGIRVRGEGTIRSVDVTT
                     LPYPGFPTDLQAQFMVLMAVAEGVSVITETIFENRFMHAQELDRMGAHIKLEGNRAVV
                     RGVRELSGAPVMASDLRASVALILAGLVAKGTTEVSRVYHLDRGYEKIERKLSQIGAQ
                     IVRVTG"
BASE COUNT          504 a          750 c          644 g          548 t
ORIGIN      
        1 cgaatcttca cattataaaa aagataggga cgtccgctca agtcgacgac cactttggcc
       61 agcgcttcat ccagcgggca gctcgcctcg ccgaagcgcc ggatcccgcg tttatcgccg
      121 agcgcgtctt tgaacgcctg gcccaaagcg aggccgacgt cttccaccgt atgatggtaa
      181 tcgactttga gatcaccttt ggcctcaata tcgatgttga aaaaaccgtg ccggctaaag
      241 ctatccagca tatgattgag aaagggcatg ccggtctcaa tcgccgcttt tcccgcgcca
      301 tcgatttcca gcgagagttt tatatccgtc tctttggttt ttcgctcgac tttcgctgtc
      361 cgtgccatcc tgatctcctt gcgtcgctcc gctgggcgcg ctgctcaact cgccgcctat
      421 acatcctgtc tgaatcgcgc ctccactgag cgcgcgtgat cgtccaatcc ttccagtctc
      481 gccagatgcg ttatcgtcgg cgccaatgcc ttcagagcgc gacgctcggc tcgaatgatg
      541 gtcgttcgct tgagaaagtc ataggtcccg agcggagaaa aaaatcgcgc gctcccgccg
      601 gtcggcagta catgattggg tccggcgaga taatcgccta aaggcggcgc cgaataaggt
      661 cccaaaaaga ttgcgccggc gttacgaatt cgcgccgcca atttttccgg ttccttcacg
      721 atcaattcga tgtgctccgg cgcaatgtcg tttgccaatt cgatcatttc ttcgatcccg
      781 ctcgccacga tgatcgcccc atattttttt agcgactgta aggtaatggc ctgccgcttg
      841 gtgcggcata aacgatcctc gatcgcggtc tggatccgct gagcggagga agaagaggtc
      901 gtgacgcaaa ttgccgccgc cagctcatcg tgttccgctt gcgatagcat gtcggcggcc
      961 acatggaccg gatcggcgga attgtcgacc aaaaccaaaa tctcgctcgg tcccgcgatg
     1021 gagtcgatat tgacctcacc aaaaaccatg cgtttggcgg tcgccacgaa aatatttcct
     1081 ggcccgacga tcttgtctac cttaggaatc gtttgggtgc caaaagccat tgccgccacg
     1141 gcctgtgctc caccgatgcg aaaaactcga ttcaccccgg caaggtgcgc cgcggcgagc
     1201 agaatggagc catcggcgcc gagcggcgaa gtcatgatga tttcatcgac tcccgccact
     1261 ctggcgggaa gcgcattcat gagcacggtt gacgggtata atgctttgcc ccctggaaca
     1321 tagataccga cgcgcttcaa cggcgtaatg cgctgcccaa gcagcatgcc cagagggtcg
     1381 cgatagcgcc agctcttctg cagctgatgc cggtgaaata tcgagatccg tttgactgct
     1441 agccgcaatg ccgctaggtc ctcacgcggc aatgtcttca tggcagcttc gatctcgctc
     1501 tgcttgactt cgacagtcga cggattgaga cggacgtgat cgaacctggc ggtataccgc
     1561 agcaccgcgc ggtcgccttg acgacggacc gcttcgataa tttccgcaac tcgccgctcg
     1621 actgagccgt cacgcgtccc acgccgctcc agaattcgtt gcaatagcgg cttgaaggta
     1681 gcctcagtgg tctttactag tctgatcggc atcccgcgca ctcactcggc gatggattta
     1741 ttcctgttga agtttgagac ttggaacaac ttagccagtt acccgaacga tctgagcgcc
     1801 gatttgcgaa agtttcctct cgattttttc gtaaccccga tccaagtgat acacgcgcga
     1861 gacctcggtg gtacctttgg cgacaagacc ggccagaatc aatgccacgc tggcgcggag
     1921 atcgctcgcc attaccggcg cacccgaaag ctcgcgcact ccgcgcacca ccgcgcgatt
     1981 tccttccaat ttgatgtgcg cgcccattcg gtccagttct tgagcgtgca tgaagcgatt
     2041 ttcgaaaatg gtttccgtga tcacgctgac gccttccgcc accgccatga gcaccatgaa
     2101 ttgcgcttgc agatcggtgg gaaagccggg gtaaggaagc gtcgtcacat cgacgctcct
     2161 aatcgttcct tctccgcgca cgcgaattcc ggcgagatca gaaacgaagt ggatgccggc
     2221 ttccttcagc ttgatcaaaa acgcatcgag gtgttcggcc cgggctcctt tgatcagcac
     2281 atcaccgccg gccagtgccg ccgctatgat aaaactgccg gcctcgatcc ggtcgggaat
     2341 gattctatgc ctgaccccat gcaaggcttt tacgccgtcg atttgaacaa tcgcgccgcc
     2401 agccccctca attcgtgcgc ccatcgcatt gagtgcacgc gccaag
//
LOCUS       DASULZ010000215         2102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_735944, whole genome shotgun sequence.
ACCESSION   DASULZ010000215 DASULZ010000000
VERSION     DASULZ010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735944"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..1710
                     /locus_tag="VFY96_04445"
     CDS             88..1710
                     /locus_tag="VFY96_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5605741.1"
                     /translation="MPRLQKIPATSWLFALALGLLLVACRGTPGSGDIRFRVNLGTEP
                     PSLDWSLATDHVSFNVIANLMVGLTEFDKNLKPAPVIANSWDVLDGGKRIVFHLRDDV
                     LWSDGKKVRAQDFEYSWKRLLNPKTASQYAYILFDIANAQDYAEGKLKDPAAVGVQAQ
                     DDQTLMVTLRHPASYFLAITTFEVTYPQRQDIVEKFANRWTETGNLDDDGRPIPVVTN
                     GPFLLSSWKHENEIELSANAKFFRGKPTIDKVTMYMVNEKTTAVAMYEQGSLDFMDES
                     HSIPTLDKPRLSKMPGYKVVPQLRGEYYGFAVDRKPFDNPKLRKAFALAIDRTVFPKI
                     LQGRAVPGTSWIPPGMLAHNPEIGLAFNPAEARKLLADAGFPDGKGLPPIVLGYNTQD
                     DHKLVAEAVQGMWQKNLNVIVKIENQEWKVYLKKLQTDPFVVHRAGWGADYPDPDNFM
                     KLFTSNSGNNHGRWKNPRYDELLEQAAREQDEKKRTRLYDQAQKLLTETDAAIVPLFW
                     TTEATLLSPKFTGLEYNSMARMDLRNVKPAAK"
     gene            1822..>2102
                     /locus_tag="VFY96_04450"
     CDS             1822..>2102
                     /locus_tag="VFY96_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605742.1"
                     /translation="MELSEDDVLHILKLIDESKFDYFQLEVGELKITVSKGDPIPIGS
                     PSGQPAAAPAPSSTVAVKPQSTSASSTPASGAAAAKPVAIPEGMLPITAP"
BASE COUNT          532 a          581 c          545 g          444 t
ORIGIN      
        1 catcgcgagg cgtacctttc ttgaaggcgc tatattattg cggtactaat accggctaac
       61 atcagtacaa gcaagacaga tagatctttg ccccgactgc aaaaaatccc agccaccagt
      121 tggcttttcg cgctcgcgct gggcttgctt ttagtcgcct gcagaggcac accgggatcc
      181 ggcgacattc gcttccgcgt caatcttggc accgagccgc ctagtctcga ttggtcgctc
      241 gcgaccgatc acgtctcttt caatgtcatt gccaatttga tggtcgggct gacggagttc
      301 gacaagaatc tgaagcccgc gccagtgatc gccaattctt gggatgtgct cgatggcggc
      361 aaaagaattg tctttcattt gcgcgatgac gtgctgtgga gcgacggcaa aaaggtccgc
      421 gcccaggatt tcgaatactc ctggaagcgc ttgctcaatc cgaaaaccgc atctcaatat
      481 gcttacatcc tgttcgacat cgcgaatgcc caggactatg ccgagggaaa gttaaaggac
      541 ccggcggccg ttggcgtgca agcgcaagat gatcaaacgt tgatggtcac gttacgtcac
      601 cccgcctctt attttctggc gatcacgacg ttcgaagtga cctatccgca gcgccaagat
      661 atcgtggaaa aatttgcgaa ccgctggacc gagacgggta atctcgacga cgatggccgg
      721 ccaatcccag tcgttaccaa cgggcccttt cttctgagtt catggaagca cgaaaacgaa
      781 atcgaactca gcgccaacgc gaagtttttt cgcggtaaac ccacgatcga taaagtcacc
      841 atgtacatgg tgaacgaaaa aaccacagcg gtcgcgatgt acgagcaagg cagcctggat
      901 ttcatggatg agtcacacag cattccgacg ctcgacaagc cgcgtttgtc gaagatgccc
      961 ggctacaaag tcgtgccgca gctacgcggt gaatattacg gctttgctgt ggaccgaaag
     1021 ccgttcgaca atccaaaatt gcgcaaggcc tttgcccttg ccattgatcg cacggtcttt
     1081 ccgaagatcc ttcaaggccg cgcggtgccg ggaacctcct ggattcctcc cgggatgctc
     1141 gcgcacaacc cggagattgg gttagccttc aatcccgccg aagcacgcaa actgttggcg
     1201 gacgccggct ttcccgacgg caaaggactg ccgccgattg tgctgggcta caatactcag
     1261 gatgatcaca agctcgtcgc tgaggcggtt cagggaatgt ggcagaaaaa tctcaatgtc
     1321 atcgtcaaga tcgaaaacca ggaatggaaa gtttatttaa aaaagctgca aaccgatcct
     1381 ttcgtcgtgc atcgcgccgg ctggggtgcc gactatcccg atcctgataa tttcatgaag
     1441 ctgtttacct caaacagcgg caataatcat ggccgttgga aaaatccccg ctatgacgaa
     1501 ttgctggagc aggccgcgcg cgaacaagac gagaaaaagc gcaccagact ttacgatcaa
     1561 gcgcaaaaac ttcttaccga gaccgatgct gcgattgtcc ctcttttctg gaccaccgaa
     1621 gcaacgttgc tgagccccaa attcaccggg ctcgaatata attccatggc gcggatggat
     1681 ttaagaaacg tcaaaccggc ggcgaagtaa tgcgcaagcc gttgaagagt cgtgaccgca
     1741 tggcattttt ttctgcaatt tgccaagccg ggtgaaatcg cataaggagt aaaagaaatc
     1801 acctcgagga aggcgacgga aatggaactc agcgaagatg atgtgctgca catattgaag
     1861 ttgatcgatg aatcgaaatt cgactatttt caactcgaag tcggcgagct caaaattact
     1921 gtcagcaagg gcgatcccat tccgatcggc tccccgagcg gacaaccagc tgctgctccg
     1981 gcaccgagtt caacagtagc agtgaaacct cagtcaacat cagcttcgag tacgcccgcc
     2041 tccggcgccg cggccgcaaa acccgtggca attccggaag gcatgctacc gatcaccgcg
     2101 cc
//
LOCUS       DASULZ010000216         5690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_634727, whole genome shotgun sequence.
ACCESSION   DASULZ010000216 DASULZ010000000
VERSION     DASULZ010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5690
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634727"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VFY96_04455"
     CDS             complement(<1..197)
                     /locus_tag="VFY96_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605743.1"
                     /translation="MEIRQGDIITNLPYHPNCTLWFDHHITNTTPDFPQPIILGRGGF
                     RLAPSAARVVHEYYCEVGTTR"
     gene            complement(311..1429)
                     /gene="ald"
                     /locus_tag="VFY96_04460"
     CDS             complement(311..1429)
                     /gene="ald"
                     /locus_tag="VFY96_04460"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX5605744.1"
                     /translation="MIIGVPAEIKTEENRVAVTPAGVTAFVARNHEVLLQSGAGLGSG
                     LTDRAYEAAGAIIVESAKEVWEKAEMVMKVKEPQESEYPLLREGLILFTYLHLAANES
                     VTRALLDRKVTAIGYETIQLDDNSLPLLAPMSEIAGRLSIQVGAWSLQAENGGRGILL
                     GGASGVRPAKVAILGAGMSGSAACQVAAGMGAYVSILDINPTKLRYVHDILGGHLTTL
                     MSNRANIEEEVMDADLVIGSVLIPGAQAPKLISRGLVRRMKPGAAFVDISIDQGGCAE
                     TSRPTTHRQPIYIEENVVHYCVTNMPAIVPNTSTYALTNATLSYGLQLANRGFVQAIA
                     RDKALAKGLNTYKGKVTHEAVANAFDLSWTPAEQAIHS"
     gene            complement(1512..1877)
                     /locus_tag="VFY96_04465"
     CDS             complement(1512..1877)
                     /locus_tag="VFY96_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEX5605745.1"
                     /translation="MDRFFFISGAVAALIGVALGAFGAHSLRTKLSPEMLGIFEVGVR
                     YQMYHAFGLIAVAWAMARWPEANLNAAGWAFIVGVFIFSGSLYLLSITDVRWLGAITP
                     IGGLAFLLGWAILVWSIGR"
     gene            complement(1906..3372)
                     /locus_tag="VFY96_04470"
     CDS             complement(1906..3372)
                     /locus_tag="VFY96_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxyl carrier protein"
                     /protein_id="HEX5605746.1"
                     /translation="MEGITFVDTTIRDGHQSLWAENMTTGMMLPVAEAMDRAGFDAIE
                     LISSSHLKKCVRELKEDPWARVKLMSERITRTPLRLNAGRFSAFDLTPRSMYRLFMER
                     MAANGIREARISEEWNELEGWIWKVAVARDVGINPVPNIIYSVSPKHTDEYFAERTRQ
                     AASLKVNRLCLKDPGGLLTPERVQSLVPIMFANANGIPIELHTHCTTGLGPLCCLEAV
                     KLGIKIVNTALPPLADGSSNPSLFNVARNLRALGYWTLIDEEILKPVSEHFTQIAKRE
                     GFPIGEPVEYDYSQYQHQVPGGMISNLRFQLRKVGVEHKIDEALRETMRVRAELGYPI
                     MVTPLSQFVGSQAAINVIVGERYKEVTDQIMQYALGYWGREGAELMDPEVKAKILDRP
                     RAKEWAAWQPPEPSIAEIRKKMNAEHVSDEELLLRWNLTGDEIEAMRATGAPKEYLTS
                     RQPLVNLIDALSKRKGYRQIVIQKGDMLVSLNRGTPAS"
     gene            complement(3496..4353)
                     /locus_tag="VFY96_04475"
     CDS             complement(3496..4353)
                     /locus_tag="VFY96_04475"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5605747.1"
                     /translation="MARDIVAIDVHVHLSDETTQKAKGDRTAQMARYFGRERPPVSID
                     EMADQYRARKMMAVIMNTRDETVTGLTPVPNDHVASAVKKHPDVFLAFGAIDPWTGKL
                     ALDEIKRCKDLGLSGMGELNPARQHFFPNDVRFYPLWEQCAKLNMPILFHGGMAAAGA
                     GTPGGMGIKLKYSQPIAIDEVAADFPELKIISAHPTWPWTAESLAIARHKANYYIDLS
                     GWAPKYFPAELVHNINTLLQDKAMFGSDWPAIGVERWLEEFQQVNMKPEVRKKIMLDN
                     AVKFLGVTL"
     gene            complement(4528..>5690)
                     /locus_tag="VFY96_04480"
     CDS             complement(4528..>5690)
                     /locus_tag="VFY96_04480"
                     /inference="COORDINATES: protein motif:HMM:NF014489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin carboxyl carrier protein"
                     /protein_id="HEX5605748.1"
                     /translation="VERMFANGIREARISDEWNDYEGWKFRVGECHRAGLKSILNLIY
                     SVSPKHSDQYFAERTRQAASLNPYRLCLKDPGGLLTPERMETLVPIIFKNANAIPVEL
                     HTHCTTGLGPLCCIKGMELGIRSINTALPPLADDSSNPSLFNVAKNARALGYATAIDE
                     EILQPVSRHFTAIAKREGFTIGAPVEYDYTQYQHQVPGGMLSNLRFQLRKVGMENKYP
                     QALEESIRVRQEFGYPIMVTPLSQFVGSQAAINVIVGERYREVTDQVIKFALGHAGAE
                     GSRDMDPNVKDKILDRPRAKEWAKWQPDDPTPDEMRRRLGAEGTSDEELLLRWIVGQE
                     DVDVMRANPRPIEYLNSEQPLVNLLQELSKRTKNRQIHVKTSGFSMTLERRA"
BASE COUNT         1199 a         1599 c         1589 g         1303 t
ORIGIN      
        1 tcccgtgtgg tgcctacctc gcagtagtat tcgtggacta cccgagcggc gcttggcgcg
       61 agacgaaagc cacctctccc caaaatgatc ggttgcggaa aatccggtgt ggtattggtg
      121 atatgatgat cgaaccagag cgtacagttc ggatgataag ggagattggt aatgatatcg
      181 ccctggcgga tttccaccaa accgtcctac atgaacttgg gctcgacaaa caggtactcg
      241 tcgatattct ccaactgggt gaccagggcg ccgcaaacca atccgtcaaa atccgcgcga
      301 gtaacgagcc tcatgagtgg atggcctgtt cagccggcgt ccacgacaga tcgaaagcgt
      361 tggcaacggc ctcgtgggta accttgcctt tataggtatt gagtcccttg gctaaagcct
      421 tgtcgcgtgc gatcgcctga acaaagccgc gattggccag ctggagccca taggagagcg
      481 tggcattggt caatgcatag gtcgaagtat tcgggacgat cgccggcata ttcgtcacgc
      541 agtaatgaac gacgttctcc tcgatgtaga tcggctggcg gtgggttgtc ggtcgcgatg
      601 tttcggcgca accaccttgg tcgatcgaga tgtcaacaaa ggccgcaccg ggtttcatgc
      661 gtctgacgag tccgcgcgag atcagtttgg gcgcttgcgc gccgggaatc agcactgaac
      721 cgatgaccaa gtcggcgtcc atgacctctt cttctatatt ggcgcgattg gacatgagag
      781 tggtcagatg tccgccgaga atatcatgca cataacgaag tttagtggga ttaatatcga
      841 gaatgctgac gtatgcaccc attcccgcgg cgacttggca agccgcgctt ccggacattc
      901 cggcgccgag aatcgcgact tttgccggac ggacgccgga cgctccgccg agaagaattc
      961 cacggccgcc attttcggct tgcagagacc acgcgcccac ctggatcgag agccggcccg
     1021 cgatctcgct catcggtgct aaaagcggca atgagttatc gtcgagctga atggtttcat
     1081 accctatggc ggtcactttg cgatcgagga gcgcgcgggt aacggattca ttggcggcca
     1141 aatgcaagta ggtgaacagt atgagtcctt cccgcagcaa tgggtactcg gattcctgcg
     1201 gttctttcac cttcatgacc atctccgcct tttcccaaac ttctttagcc gattcgacga
     1261 tgatcgcgcc ggctgcttca taagcgcgat ccgttagacc gctgcccaat ccggcgccgc
     1321 tttgcagaag cacctcgtga ttacgcgcca caaaggcggt cactcccgcc ggcgttaccg
     1381 caacacggtt ttcttcggtc ttgatctccg cgggaacgcc gatgatcatg gttacgaaaa
     1441 actacgtgaa ggccagggtg gagtcaacgt atgaggactg gagtactgaa gtgtgggagt
     1501 gttgatccca atcaccggcc gatcgaccaa acgagaatcg cccagccgag cagaaatgcg
     1561 agcccgccga tcggcgtgat cgcgccgagc caacgtacat cggtgatgct cagaagatac
     1621 aggctgcccg agaagataaa aacgccgacg atgaaagccc aaccggcggc gtttagattc
     1681 gcctcgggcc agcgcgccat tgcccatgct acagcgatca agccaaaggc atgatacatc
     1741 tgatagcgca cgccaacttc gaaaataccg agcatctccg gcgaaagctt tgtccggagt
     1801 gaatgagcgc cgaacgcgcc gagggcgacg ccaatcagtg cggcgacggc gccggaaata
     1861 aaaaagaagc gatccatgtg caaggcccag gagtaacttt ggattttagc tggccggtgt
     1921 gccgcgattg agtgacacga gcatgtcgcc tttttggatg acgatctggc gatagccttt
     1981 gcgtttgctc aaggcatcga tcaggtttac cagcggctgg cgcgaggtga gatactcttt
     2041 cggcgcgccg gtcgcgcgca tcgcttctat ctcgtcgcca gtgagattcc agcgcaagag
     2101 cagctcttcg tcggacacat gctcggcgtt catctttttt cggatctcgg cgatgctcgg
     2161 ctccggcggc tgccacgccg cccattcctt cgcgcgcggc cgatcgagga ttttcgcctt
     2221 gacctccgga tccatcaatt ccgcaccttc tcgaccccag tagccgaggg cgtactgcat
     2281 gatttgatcg gtgacttcct tataacgctc gccgacgatg acgttgatcg ccgcctggct
     2341 gccgacaaac tgggagagtg gcgtgaccat gatcggataa ccgagctcgg cgcgtacgcg
     2401 catggtttct ctcaaggctt cgtcgatctt atgctccacc ccgactttgc gcagctgaaa
     2461 acgcagattg gaaatcatgc cgccggggac ctgatgctga tactggctgt agtcgtattc
     2521 caccggctcg ccaatgggaa atccttcccg tttggcaatt tgggtgaagt gttctgacac
     2581 cggcttcaga atttcttcat cgatcagcgt ccaataacca agcgcgcgca aatttcgcgc
     2641 gacattgaat agtgacgggt tcgacgaacc atccgccaat ggcgggagcg cggtattgac
     2701 aatcttgatt ccgagcttca ccgcttcaag acagcagagt ggacccagac ctgtggtgca
     2761 gtgtgtgtgc agctcgatgg gaatcccatt ggcgttggca aacatgatcg gcaccaagct
     2821 ttgcacgcgc tcgggagtga gcagtccacc cgggtctttc aggcagagcc ggttgacttt
     2881 tagcgaggcg gcttgccgcg tgcgctccgc aaaatattca tccgtatgtt ttggcgaaac
     2941 ggagtagata atgttcggca ccgggttgat gccgacatcg cgcgccacgg caaccttcca
     3001 gatccagcct tcaagctcgt tccattcttc ggagatgcgc gcttctcgaa taccgttggc
     3061 ggccattctt tccatgaaga gccggtacat cgagcgtggg gtgagatcga aggcgctgaa
     3121 ccggccggca ttcaatcgca gcggcgtccg ggtgatgcgc tcgctcatga gctttacccg
     3181 cgcccaggga tcttctttga gctcgcgcac gcactttttc aaatgcgagc tggagattaa
     3241 ctcgatggcg tcgaagccgg cgcgatccat ggcttcggca accgggagca tcatgcccgt
     3301 ggtcatgttt tccgcccaca ggctctgatg cccgtcgcgg atcgtagtat caacgaatgt
     3361 tattccttcc atcgttccat tcagcggatc tcagggggga agttttacca caaaggcagc
     3421 gaagttcacg aaggtttttt cgcaatcctc ttcgtcctct tggtgcgctt cgtaataagt
     3481 ccctgagtta ctgctctaaa gtgttacgcc caagaatttc actgcattat cgagcatgat
     3541 tttctttcga acttcaggtt tcatgttgac ctgctgaaac tcttccagcc agcgctcgac
     3601 gccaatggcc ggccaatcgg agccgaacat cgccttgtcc tgcagtaggg tattgatgtt
     3661 gtgcaccagc tccgcgggaa aatatttcgg cgcccaccct gacaaatcga tgtagtaatt
     3721 tgctttgtgg cgcgcgatcg ccaagctctc agcagtccac ggccacgttg ggtgagcgct
     3781 gataattttc aactccggga aatccgccgc gacttcgtcg atggcgatcg gttggctgta
     3841 ttttagcttg atgcccatgc cacctggcgt gcccgccccg gctgccgcca tcccgccgtg
     3901 gaacagaatt ggcatgttca gtttggcaca ctgttcccat aagggataga agcgcacatc
     3961 attgggaaaa aaatgttggc gcgccggatt gagttcgccc atgcctgaaa gccccaagtc
     4021 tttacagcgc ttgatttcgt ccaacgcgag ctttcccgtc cacggatcga tcgcaccgaa
     4081 ggcgagaaaa acatccggat gctttttcac cgcgctcgcc acgtgatcgt tgggaaccgg
     4141 cgttaaacca gtgacggttt cgtcgcgcgt gttcatgatc acggccatca ttttacgcgc
     4201 ccgatattga tcggccattt catcgatgga gactggaggg cgttcgcggc cgaagtaccg
     4261 cgccatttgt gcggtgcgat cacctttggc cttctgcgtt gtttcatccg aaagatgaac
     4321 gtgaacatca atcgcgacga tatctcttgc cataattttt ggcctcctca gcacgatttc
     4381 tttggttatt gcaccgcgac ggaattagtt caaggttccg tgctcaaggg atgtcctgaa
     4441 cggagtgaag gatcattgcg tactgtaaga tccttcgaac tcacacgtgg gggcgcgctc
     4501 ttgaaactaa atgtccgcac gaagctccta cgcccgccgc tccagcgtca tcgaaaaacc
     4561 ggacgttttc acgtggatct ggcggttctt ggtgcgttta gaaagttcct gtagcagatt
     4621 cacgagcggc tgctcggagt ttaaatattc gatgggccgc ggattggcgc gcatgacgtc
     4681 gacgtcttcc tgaccgacaa tccagcgcag caggagctcc tcgtcggacg tgccttcagc
     4741 gccgagccgc cggcgcattt catccggtgt cggatcgtca ggctgccatt tggcccactc
     4801 cttcgcgcgc ggacgatcga gaatcttatc tttcacgttc ggatccatgt cgcgcgaacc
     4861 ctcggcgccg gcatgaccaa gcgcgaattt aatcacttga tcggtaactt ctcgatagcg
     4921 ctcgccgaca atgacattga tcgcggcctg gctgccgacg aattgtgaaa gcggcgtcac
     4981 cataatcggg tagccgaact cttgacgtac ccgaatgctc tcttccagcg cctgcggata
     5041 cttgttttcc atgccaactt tgcgcaactg aaatcgtaaa ttggagagca ttccgcccgg
     5101 gacttggtgc tgatactgcg tgtagtcgta ttcgaccggc gcgccgatcg taaatccctc
     5161 gcgtttggca atggccgtaa aatgccttga gaccggctgc agaatttcct catcgattgc
     5221 cgttgcgtat ccgagggccc gggcattctt cgcgacattg aagagagagg gatttgacga
     5281 gtcatcggcc aatggcggaa gtgccgtgtt gatcgagcga atgcccagtt ccatgccttt
     5341 gatgcagcag agcggcccca atccagtcgt gcagtgggtg tgcaattcca ccggaattgc
     5401 attcgcattc ttgaagatga tcgggaccaa ggtctccatc cgctcgggtg tgaggagccc
     5461 gcccggatcc tttagacaaa gccgatacgg attgagtgag gccgcctggc gggtgcgctc
     5521 ggcaaaatat tggtccgaat gtttgggcga aaccgaatag atcaaattta atatcgattt
     5581 gaggccagcg cgatggcact cgccgacgcg aaatttccag ccttcgtaat cgttccattc
     5641 gtccgatatg cgcgcctccc ggataccatt ggcaaacatg cgctcgacaa
//
LOCUS       DASULZ010000217         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_330110, whole genome shotgun sequence.
ACCESSION   DASULZ010000217 DASULZ010000000
VERSION     DASULZ010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330110"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VFY96_04485"
     CDS             <1..798
                     /locus_tag="VFY96_04485"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5605749.1"
                     /translation="IQRVNRMKVVVRGLTKVYTTNQGGELRAIGGINLDVRDREFFGI
                     IGPTGCGKTTLLQIIGRLEAPSTGTVEFVGEQHAKSPVSMVFQDSALMPWRTVEENVP
                     LGAEFRKEQPSVLKRIGQVFLEIVRLADFAGARPHELSGGMKQKVAIARALANDPEVV
                     LMDEPFANLDAQTRLVMREELLRIWERDKKTVILVTHNLEEAVMLCDRIAVMSSRPGL
                     IKEVVTVDIPRPRTMKSMRDPDFSLCMEKIWNLLRYDVDAALKSNRI"
     gene            1021..1743
                     /locus_tag="VFY96_04490"
     CDS             1021..1743
                     /locus_tag="VFY96_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX5605750.1"
                     /translation="MRAFSWFHQASFLAIVVWFGGCAASSSAPDYSAALKDPDRPQAE
                     VELDAARKPQEVLAFYGVKSGDKVADIYAGRGYYTAILSNVVGPRGVVYTINPNSRDE
                     VNQRWKSAKFANVKIADGPLDKIALPQDGSLDFALTHLNYHDLSPEVRMAMNKRIFAA
                     LKHGATYAVVDHSAKEGSGNEATKTLHRIDKTLVIKEISAAGFKLAKEGNMLRKPEDT
                     RDFNVNKERNKDDRFVLAFQKP"
     gene            1766..>2264
                     /locus_tag="VFY96_04495"
     CDS             1766..>2264
                     /locus_tag="VFY96_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5605751.1"
                     /translation="MPMRLRNYVRLISALFFLSLFVGCAASSTPDYKAVLGDTNRTEA
                     ERSLDAVRKPNEVMAFYGVKNGDRVADIFASRGYYTAILSQLVGPSGIVYSANLAPRQ
                     ELHDRAKQPAMANVRIIDGSFEQIALPQDGSLDFVFIHLDYHELPPDARVPMSRRIFN
                     SLKKGG"
BASE COUNT          523 a          609 c          644 g          488 t
ORIGIN      
        1 atacagcgag taaaccggat gaaagttgtt gttcgcggat tgacaaaggt ttacacgacc
       61 aatcaggggg gggagctacg cgctatcggt gggatcaatc tcgatgtccg ggatcgcgag
      121 tttttcggga tcatcggtcc aaccggatgc ggcaaaacga cgctgttgca gatcattggc
      181 cgtctggaag cgccgagcac cggtacggtg gaatttgtcg gggagcaaca tgcgaaatcg
      241 ccagtatcga tggtgttcca ggattccgct ctgatgccct ggcgtacggt tgaagaaaat
      301 gtcccgctcg gcgccgagtt ccgcaaagag caaccgtcgg tgctaaagcg gatcggccaa
      361 gtgttcttgg agatcgtgcg cctcgccgac tttgccggcg cgcgaccgca tgaactctct
      421 ggcggcatga aacagaaggt cgctatcgcg cgtgccttag cgaatgatcc cgaagttgtg
      481 ctcatggatg agccgttcgc caaccttgac gcgcaaacgc ggctggtcat gcgcgaagag
      541 cttctgcgga tctgggagcg cgacaaaaaa accgtcattc ttgtcaccca caacttggaa
      601 gaagccgtga tgctctgcga ccggatcgcg gttatgagct ccaggccggg gctcatcaaa
      661 gaggttgtga ccgtggacat tcccaggccg cgcaccatga aaagcatgcg cgacccggat
      721 ttctcgctgt gcatggaaaa gatctggaac ttgctccgtt acgacgtcga tgcggcactg
      781 aagagtaacc gaatatagtg tcttccgact agacaataat gctcttcggc atagcgacga
      841 aagtgctgga ttccggatcg cactagctat cgccagttgc cccggacgac cgccttcgtt
      901 gttgtacttc ggcacaaata gcagaattgg cagctcggtt gtcctcctga ccctgatccc
      961 ttgcttttcg cttgtgacaa tctacattca aacgacagat aaactcagga ggcgcgattt
     1021 atgagagctt tctcgtggtt ccaccaagca tcttttctgg cgatcgttgt atggtttggc
     1081 ggttgtgccg caagcagtag cgcgccggac tactccgcgg cgctcaagga tccagacagg
     1141 ccccaagcgg aagtcgaact cgatgcggcg cgcaaacctc aggaggtgct ggctttttat
     1201 ggtgtcaaga gcggcgacaa agtcgcggat atttatgccg ggcgcggtta ctacacggcg
     1261 atcctttcca atgtagttgg accgcgggga gtcgtataca cgataaatcc gaactcccgg
     1321 gatgaggtca atcagcgctg gaaatcggct aagtttgcca acgtcaaaat tgccgatgga
     1381 ccgctcgata aaatcgcctt gccgcaggat ggctcgcttg atttcgcgtt gactcatctc
     1441 aactaccacg acctttcgcc cgaggtgcgc atggccatga ataaacgaat cttcgcggca
     1501 ttaaaacatg gcgctactta cgcggtcgtg gatcattcgg cgaaagaagg cagcggcaac
     1561 gaagcgacca agaccctcca ccggatcgac aagactctgg tgatcaagga aatcagcgcc
     1621 gccggtttta agctagccaa agaagggaac atgctgcgca agcccgaaga cacgcgcgac
     1681 ttcaacgtca acaaggagcg caacaaagac gatcgtttcg tgctggcgtt tcaaaaaccg
     1741 taaaatctgg catcgaggag acatcatgcc tatgcgactg cgcaattatg tcaggttaat
     1801 ttcggctctg tttttcctat cgctctttgt cggttgcgcc gcgagctcga cgccagatta
     1861 caaggccgtt ctcggcgaca cgaaccgcac cgaagccgag cggtcgctcg atgcggttcg
     1921 caagcccaat gaagtgatgg cgttttacgg cgttaaaaac ggggacagag ttgccgatat
     1981 ttttgccagc cgcgggtatt atacggcgat tctttcgcag ttagtcggac cgagcggaat
     2041 agtttattcg gcgaatctcg cgccgcgcca ggaactgcac gatcgcgcga agcagccggc
     2101 catggcgaac gtgcggatca tcgacggatc cttcgagcaa atcgcgctgc cgcaggacgg
     2161 ttcactcgac tttgttttca ttcatctcga ttatcacgaa ctgccgcccg atgcccgggt
     2221 cccgatgagc cgccggattt tcaactcact caagaaaggt ggaa
//
LOCUS       DASULZ010000218         2622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1040016, whole genome shotgun sequence.
ACCESSION   DASULZ010000218 DASULZ010000000
VERSION     DASULZ010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040016"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VFY96_04500"
     CDS             complement(<1..758)
                     /locus_tag="VFY96_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEX5605752.1"
                     /translation="MALKTFGLMDVDWEERVNIDRLRQERLERIKVALKKSELGALLC
                     FDMNNIRYITATHIGTWAMDKLVRFSLLAQEDEPILWDFGSAARHHQIYCPWLGERSR
                     AGISTLRGATNPGSALASDVAKKIKRELEKRHLQSEPVGVDVIEMPVLFALQAEGIKV
                     VDGQQLMHEVRKIKTQDEITLLNTACMMVDSAYDELYRFMRPGVKENECVGVVSKVLY
                     DLGSEHVEGVNAISGERCSPHPHVYTDRVLRPGDP"
     gene            complement(826..1806)
                     /locus_tag="VFY96_04505"
     CDS             complement(826..1806)
                     /locus_tag="VFY96_04505"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX5605753.1"
                     /translation="MRQISFAQAINEALAEEMRRDPTVFIIGEDVAEAGTPFKVLSGL
                     VKEFGTKRVIDSPISEAGIAGIGVGAAMTGMRPVVDIMFGDFLTLTMDQLVNQAAKIH
                     YMSGGKLKVPLVLRTTLGASRRSAAQHSQSLHAWASHIPGLKVVVPATPYDAKGLLKS
                     AIRDNNPVVFFEDKMMYQLKGPVPLEEYTIALGVADIKRAGTDITLVATSSMVQVALE
                     AAGSLHEADISAEVIDVRTTVPLDKETLIGSVKKTSRAMVIDEGYESYGVTAEIASVI
                     AAGAFYYLDAPVMRMGAMDVPVPFSPVLEDQTVPTSEKVAETALVLCNKR"
     gene            complement(1939..>2622)
                     /locus_tag="VFY96_04510"
     CDS             complement(1939..>2622)
                     /locus_tag="VFY96_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014132772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX5605754.1"
                     /translation="GKGGSMHIADQESGNLGANAIVGGSTGIATGAALSARMRGTEQV
                     AVCFFGDGALGQGLLYESMNMAALWNLPVIYVCENNQYNEYTHYSETTAGPLTARAEA
                     FGIQTEAVDGQDVRIVHQVAVDSVAKARQGEGPSFILCNTYRFHGHHVGDVDRAYYRS
                     KQEEEEWKSQRDPVQILAHWLLKTKLADSSSLARIQSTIAADIKRAVGAALEAPYPEI
                     DEVREDVYA"
BASE COUNT          543 a          775 c          682 g          622 t
ORIGIN      
        1 ggatcgcccg ggcggagcac acgatcggta tagacgtgtg gatgaggact gcagcgctca
       61 cctgagatgg cattaacgcc ctcgacgtgc tccgaaccga ggtcatacag aactttgctg
      121 acgaccccga cgcattcgtt ctcctttact cctggtctca tgaatcgata gagctcgtca
      181 tacgcggaat cgaccatcat gcaggcggtg ttcaagagcg tgatttcatc ctgagttttg
      241 atcttacgca cttcgtgcat gagctgctgg ccatcaacga ccttgatccc ttcggcttga
      301 agcgcaaaaa gcaccggcat ctcgatcacg tcgacgccaa ccggctcgct ttgcaggtga
      361 cgtttctcca gctcccgctt gatctttttc gccacgtcgc tggccaaggc cgaaccggga
      421 ttcgtagcac cgcgcaaggt cgagatcccc gcccgggagc gctccccgag ccagggacaa
      481 taaatctgat gatggcgcgc cgctgagcca aaatcccaca aaatcggctc gtcttcctgc
      541 gccaacaggg agaagcgtac cagtttgtcc atcgcccaag tgccaatatg ggttgccgtg
      601 atgtaacgaa tattgttcat gtcgaagcac aggagcgcgc ccagttcgga ttttttcagc
      661 gccaccttga tgcgctcaag acgctcctgg cgcagtcggt cgatgttgac ccgctcttcc
      721 caatcgacgt ccatgaggcc gaaggtcttt aacgccatga cctaaactcc ttcgttcgag
      781 gaatcgacag tttaaccgaa ttgaaacggt taaacgtgtt gaacttcatc ttttgttaca
      841 gagcaccagc gccgtctcag cgactttttc cgaggttggg accgtctgat cttcgagcac
      901 cggcgagaat ggtacgggca cgtccatcgc tcccatgcgc atcaccgggg catcgagata
      961 gtaaaaagcg cctgccgcga tcaccgacgc gatttctgcg gtgacaccat agctttcata
     1021 tccttcatcg atgaccatcg ctcgactggt ttttttgacg gaaccgatca aagtctcttt
     1081 gtcgagcggc acggtggttc ggacgtcgat aacttcggcg ctgatatcgg cttcgtgcag
     1141 actgccggcg gcctctagtg cgacctgcac catactactg gtcgcgacca atgtaatatc
     1201 cgtgccggcg cgcttgatat cggctacacc gagcgcaatc gtgtattctt ccagcggcac
     1261 cgggcctttt aactgataca tcattttgtc ttcgaaaaag accaccggat tattatcacg
     1321 gattgcgctc ttgagcagac ccttggcgtc atagggtgtg gcgggcacga ccactttcaa
     1381 tcccggaata tgactcgccc acgcatgcag gctctggctg tgttgcgcgg cagaacgacg
     1441 gctcgctccc agagttgttc gcaacaccag cgggactttg agcttgccgc ctgacatgta
     1501 atgaatcttc gccgcctggt tgaccaattg atccattgtc agggtcagaa aatcgccgaa
     1561 catgatgtcc accaccggac gcatgccggt catcgccgca ccgacaccga tcccggcaat
     1621 gcccgcctcc gaaatgggcg aatcgatgac gcgtttggtg ccgaattctt tcaccagtcc
     1681 cgatagaact ttgaagggcg tccccgcttc ggcgacatct tcgccaatga tgaacaccgt
     1741 tggatcgcgg cgcatctctt cggcgagggc ttcgttgatc gcttgagcaa agctaatctg
     1801 cctgatcttt gtgtcggaaa cggagctatt ctcgctgctg actgtcatga aaatttgaag
     1861 tatcgggtaa gggacacact ctgccgattt cgcatttcga cttttcccgg ctaaggtaga
     1921 actgaaacgc gcaggtgatc aagcgtagac atcttctctg acttcgtcga tttccggata
     1981 cggcgcttcc aaggcggcgc cgacggcccg ctttatatcc gctgcaattg tgctttggat
     2041 ccgagcaagc gagctgctat cggctaattt ggtcttgagc agccaatgcg cgaggatttg
     2101 aacgggatcg cgttggcttt tccattcctc ttcttcttgt tttgaccgat agtaagctcg
     2161 atcaacgtct ccgacatgat ggccgtgaaa acggtacgta ttacacagga taaagctcgg
     2221 cccctcgccc tggcgtgcct tcgcaaccga atctaccgcg acttgatgta cgatccggac
     2281 atcctggccg tcaaccgctt cggtctgaat tccaaatgct tccgcccggg cggttagcgg
     2341 cccggccgtg gtctcgctgt agtgtgtgta ctcattgtac tggttgtttt cacatacgta
     2401 gatcactggc aggttccaga gcgccgccat attcatcgac tcgtagagca gtccttgacc
     2461 caaagcccca tcgccaaaaa aacaaaccgc gacctgctcg gtgccccgca ttcgggccga
     2521 taatgccgcg ccggtggcaa tacccgtgct gccgccgaca attgcgttcg cgcccaggtt
     2581 accgctttct tgatcggcga tatgcatcga tccgcctttg cc
//
LOCUS       DASULZ010000219         2887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_838302, whole genome shotgun sequence.
ACCESSION   DASULZ010000219 DASULZ010000000
VERSION     DASULZ010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838302"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VFY96_04515"
     CDS             <1..285
                     /locus_tag="VFY96_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605755.1"
                     /translation="VDSIDAVAKDMNVAVFRRFALMRHWVSSGRYKIEDLLSADGLHM
                     NDLSYGCLANLLADSLTAATEFPTEKKVEVHQVNVRVDRNSQITRQKPAQ"
     gene            complement(291..>2887)
                     /locus_tag="VFY96_04520"
     CDS             complement(291..>2887)
                     /locus_tag="VFY96_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013818103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HEX5605756.1"
                     /translation="NSKAAGPSGEYVHLSPNGRGIDGALLDLEYQRLELIKFNLFDNS
                     GTFEQYIKGHDSTPGAALKVWEEMRLPKNNPLYAAVGGNGPQLCRGELIRARMLTGIC
                     NDIKNPLMGSTGQMFARNVEFDTTFPESGNNLLIKNRHGDRLGLFKPDPQVISRKLLT
                     RGQSDPAKCNNGQGLAGNSPQANCDYKKAPFLNVLAAFWVQFMTHDWFSHLEQGSNGP
                     ELMPMGCTTEIEAGVEKSLTPERMKQLNCRPEDRIDKGLIGEETQPSSFTYAGKEYLS
                     RAPRATQNKNTAWWDASQLYGFDENSRRRVKRDPGDPAKFLLVSGPSGETYLPLLQSS
                     DPMNAQWAGQESVAFADNWSVGLSFFHNIFAREHNLFVDAFRERAAQTPDADSGLRNP
                     ATPSRIIEYKDVTADDLFEAARLVVAAEIAKIHTTEWTPQLLYNEPLFLGMNANWHGL
                     LKDFPRAREALEKIVVQNFGPSEDVKEATEWYSVFASGSGIFGLGNHVYADEPIFATF
                     DPAKKDLWDLSNPDHVNGGINHFGSPFNFPEEFVSVYRLHSMLPDLIEFRDLNGNPNV
                     ISGKIPVLETIRGKATKVLREGGLANWGLSMGRQRLGALTLQNSPLFLQNLSMPRLPG
                     GSGKVDVLALDLVRDRERGIPRFNEFRRQYGLKQLTNFDDFADPHLPKDSAELAEQRR
                     LIGTLRESYGQHRCDAAKVITKAQVNDDGSPINDCLGHTDGSMVDNIEDVDVVVGMLA
                     EFARPHGFAISETQFQVFILNASRRLFSDRFFTSSFRPEFYTQLGYDWVLNNGPEKRI
                     ENGLPNGHQIEVMPLKRVLMRTIPELRSELASVIDAFDPWARDRGEYYSLQWKPRQGA
                     ESDESFKP"
BASE COUNT          650 a          815 c          768 g          654 t
ORIGIN      
        1 gtcgattcca tcgacgcggt ggccaaagat atgaatgtgg cggttttccg gcgctttgcc
       61 ctgatgcggc actgggtatc gagcgggcgg tataaaatag aagatctcct ctccgctgac
      121 ggcctgcata tgaacgacct gagctacggc tgtctcgcca acctgctcgc agacagccta
      181 accgcggcga ctgagttccc taccgaaaag aaggtcgaag tccaccaggt caacgtccga
      241 gtagatcgca atagccaaat tacccggcaa aaacctgccc aataatacga tcagggtttg
      301 aacgattcat ccgattcggc gccctggcgt ggcttccatt gcagggagta atactcgccg
      361 cggtcacgcg cccacggatc gaaggcatcg atgaccgaag caagctcact gcgcagctct
      421 ggaatcgttc gcatcagcac ccgtttcagc ggcatgactt caatttgatg gccgttcggc
      481 aagccgttct cgatcctctt ttctggcccg ttgttcaata cccagtcata tccgagttgg
      541 gtatagaact ccgggcgaaa gctggaggta aagaaacgat cgctaaaaag acgacgcgac
      601 gcattaagga taaacacctg gaactgcgtc tcggaaatcg caaatccatg cggccgggcg
      661 aattctgcga gcatgccgac gacgacatcc acatcttcga tgttgtcaac catgctgcca
      721 tccgtatgtc ccaaacagtc gttgatcgga ctaccgtcat cgttcacttg agctttagtg
      781 atcactttgg cggcatcaca ccgatgttgg ccgtaacttt cacgcagagt gccgatgagc
      841 cggcgctgct cggcaagctc ggccgagtcc ttcggcaaat ggggatcggc aaaatcgtca
      901 aagttggtca actgtttgag accgtactgg cggcgaaatt cgttaaaccg cggtatgccg
      961 cgctcgcgat cacgaaccag atcgagggcg agaacgtcga ccttgccgct gccgccgggc
     1021 aaacgcggca tcgacaggtt ctgcaaaaaa agcggactgt tctgcaaggt caaagctccc
     1081 aatctttgcc gccccatgga aaggccccag tttgcgagtc ctccctcgcg cagcaccttc
     1141 gtggccttgc cacgaattgt ttccagcacg ggaattttcc cgctgatcac gttaggattc
     1201 ccattgagat cgcgaaattc gatcaggtcg ggcagcatcg aatggaggcg gtaaacactg
     1261 acaaattcct cggggaaatt gaatggcgaa ccaaaatggt tgatgccgcc gttcacatga
     1321 tcggggttgg acaaatccca cagatctttt tttgcaggat cgaatgtggc gaaaatcggt
     1381 tcatccgcgt aaacgtgatt gcccaaacca aaaattccgg aacccgaggc gaatacggaa
     1441 taccactcgg tggcttcctt gacgtcttcc gacggaccga agttctggac aacaattttt
     1501 tccaaggctt cgcgcgcgcg cggaaaatcc ttcagcaagc cgtgccagtt cgcgttcatg
     1561 cctaagaaca ggggctcgtt gtagagcaat tgtggcgtcc actcggtagt atgaatcttc
     1621 gcgatctccg ccgccacgac caaacgggca gcttcaaaca aatcatcggc ggtcacatct
     1681 ttgtactcga tgatgcgcga tggagtagcc ggatttcgga gaccagagtc tgcgtccggc
     1741 gtttgagcgg cgcgctcacg aaaggcgtcg acgaacagat tatgctctcg tgcgaaaata
     1801 ttatggaaga agctcagacc cactgaccag ttatccgcga atgcgaccga ttcctgtccg
     1861 gcccactggg cgttcatggg atcggagctc tggagcaaag gcaaataggt ctcccccgat
     1921 gggccgctca ccaacagaaa ctttgccgga tcgcccggat cacgcttgac gcgccggcgc
     1981 gaattctcgt caaagccgta gagctgcgaa gcgtcccacc atgccgtgtt tttgttttgc
     2041 gtcgctctcg gcgcccggct caagtactcc ttaccggcat aagtgaaact cgacggctgc
     2101 gtctcttcgc cgatcaaacc tttgtcgatt cgatcttccg gccggcagtt gagttgtttc
     2161 attcgctctg gcgtcaatga tttctcaacg ccggcttcga tctctgtcgt acagcccatg
     2221 ggcatgagtt ctgggccatt gcttccctgt tcaagatggg aaaaccagtc atgggtcatg
     2281 aactgcaccc aaaacgccgc tagtacgttc aagaaaggcg cttttttgta atcgcaattg
     2341 gcttggggcg aattgcccgc caggccctgc ccgttattgc atttcgccgg atccgactga
     2401 cctcgcgtca ataatttgcg gctgatcacc tgcggatcgg gcttgaataa tccgagacga
     2461 tcgccgtgtc ggttctttat aagcaaattg tttcccgact caggaaaagt tgtatcgaat
     2521 tccacgttgc gcgcgaacat ttgcccggtc gagcccatca atggattttt gatatcgttg
     2581 caaatcccgg taagcatgcg cgcgcggata agctcgccgc ggcaaagttg cggcccattg
     2641 ccgccgaccg ctgcatacaa tggattattt ttcggcagcc gcatctcttc ccagaccttc
     2701 agcgcggcgc caggggtact atcgtgaccc ttaatgtact gttcgaatgt accgctgtta
     2761 tcgaagagat tgaacttgat gagctcgaga cgctgatatt cgaggtcgag cagtgcgccg
     2821 tcaatgccgc ggccgttggg gctcaaatga acgtactctc cgctcggacc cgcagctttg
     2881 ctattgc
//
LOCUS       DASULZ010000220         4008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_534129, whole genome shotgun sequence.
ACCESSION   DASULZ010000220 DASULZ010000000
VERSION     DASULZ010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534129"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFY96_04525"
     CDS             <1..297
                     /locus_tag="VFY96_04525"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX5605757.1"
                     /translation="AISKKGGVIGVWPLLRRRDNFQTYIRELDYVKNLVGADHVGIGT
                     DLFGIASETAIPTHKEFALIPAALLNRGYRESDVEKIVGGNFMRVFREVARRTG"
     gene            539..1522
                     /locus_tag="VFY96_04530"
     CDS             539..1522
                     /locus_tag="VFY96_04530"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605758.1"
                     /translation="MSLKRLRFIILISALFVSPAVIRAAQTAIPLRLLYPSFAGSWAT
                     AWIAKEAGYFANEGLDVELIRVGGSTRMVAALLGGSAPIIQAGASAALAATAAGSDVI
                     IIGSTGTVSPFRLMARPEIKNPAELKGKRAGITTFGSTSDQIVRMALKHFNLEPNKEV
                     ALLSFGAQPEVFAALQSGAVQAGALSFPLYAKATQLGMRELINFGELGIEDINGTVIT
                     TRSYIAQHRDIVMRFLRAFTRGMQRYRTDKEFSKKVLAKYGKIQDDDILEGTWQDYAP
                     TLQKTPRPSLKAVQFLLDNQFPGKKLKAEQFVDLSLVEQLEKNGFFDSVTK"
     gene            complement(1689..2615)
                     /locus_tag="VFY96_04535"
     CDS             complement(1689..2615)
                     /locus_tag="VFY96_04535"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEX5605759.1"
                     /translation="MGYTNVRHYVGGLADWKESGGSIECNTPSVAMPETFSVHQQKTA
                     HRLSWLEVVANWPLERLVGFWLGMILIFGVIYWLAGIGMGWGLQAGSSVVHPDASGLA
                     TAIYFSFVTALSIGYGDVIPVGPLRILAVAEGIAGLLVFGCVISKLVSRRQEELTAEI
                     HRTTFEDRLDRVRTNLHLVFSDLGSVQQLQSEQGALPHQVLRRLESTVRVFRGELQTI
                     HDLLYRSNIEPDEETLESILANLLICLQSLVELSDSCPEENGESANLDLALRAIGNLA
                     REICGECVPRVYAPALKSAMDQIQALAERLTE"
     gene            complement(2652..2894)
                     /locus_tag="VFY96_04540"
     CDS             complement(2652..2894)
                     /locus_tag="VFY96_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5605760.1"
                     /translation="MAKLLSTEPSNEHQMISRDELRARLRDRSLIILDVLPKGAYAEA
                     HIPSAVSLPLAEIETKARQLIANLNQEIAVYCGGAT"
     gene            3125..3949
                     /locus_tag="VFY96_04545"
     CDS             3125..3949
                     /locus_tag="VFY96_04545"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5605761.1"
                     /translation="MRTELISIPTPTHPLDAVYYTPDGPGKGAVMYCHGNQMNFYVCA
                     ARFLAPHITTLGYDFMPFNRRGHDSVSTYDSRECVGGAYQTVAEGIEDNEIAANYLAA
                     RGDNSPIVIGHSNGGVLASEHVARHPQTRALILLSAHGGGNRLGNPSSARNFSLAGDT
                     AALTRQAEALVSAGKPRQLMLVPAWWWVISARTFLDRLTNAPDLVENAKRIRCPVLFI
                     RGDQEPKENYPAERFQENCSGPCEVVIVPNCDHFYVGAEENVAKIVTEWLNRTLGE"
BASE COUNT          888 a         1143 c         1081 g          896 t
ORIGIN      
        1 gccatcagta aaaaaggcgg cgtcatcggc gtctggccct tgctgcgccg gcgcgacaat
       61 tttcagactt acatccgcga gctcgactac gtaaagaact tggtcggcgc agatcacgta
      121 ggcatcggca ccgatctttt cggcatcgcc agcgagactg cgattccgac tcacaaagag
      181 ttcgcgctga ttcccgcggc cttattgaac cgtggttatc gggagagcga tgttgaaaaa
      241 attgtcggcg gcaacttcat gcgggttttt cgcgaagtgg cgcgccgtac cggatagagt
      301 tccgggtcgg gctgcgcgtc gaagacccgt aaaccgcttg ttttaatttt cggtaagaga
      361 aacccatgat ctttcatgcc gagtccttgg gcccgcatca aaataaactc tgcgaagcat
      421 ctcgcatttg gttgctttgc aagacaatgc ttcgcctatc ggctcaggac gacatgaaat
      481 tttttcgcga cgattaagca tgtctgctaa ttcccacttc tcagaacacg gaggtttaat
      541 gtcactcaaa cgtttacgtt ttatcattct gatttcagcc ttgttcgttt caccggcggt
      601 tattcgtgcc gctcagacag cgattcctct ccgacttctc tatcccagct ttgccggctc
      661 atgggctacc gcctggatcg cgaaggaagc gggttatttt gccaatgaag ggctcgacgt
      721 cgaattgatt cgcgtcggcg gcagcacgcg catggtggcg gcgttgctcg gcggcagcgc
      781 gccgatcatt caggccggag catcggccgc attggccgcc actgcggccg gtagcgatgt
      841 gatcattatc ggttcgaccg gcaccgtttc tccttttcgt ctcatggcgc gccccgaaat
      901 caaaaaccca gccgaactga agggcaaacg tgccggaatt acgacctttg gctcgacctc
      961 ggatcaaatc gtgcgcatgg ccttgaagca cttcaatctc gaacccaaca aggaagtcgc
     1021 gctactctcg ttcggagccc agccggaagt tttcgctgcg ctccaaagcg gcgccgtgca
     1081 agcgggagcg ttgagctttc cgctctatgc caaagcgacc cagctcggca tgcgcgaatt
     1141 gatcaatttt ggcgagcttg gaattgaaga tatcaacggc acagtgatca cgacccgtag
     1201 ctacatcgca caacaccgcg atatcgttat gcgttttctt cgcgccttca cccgcggcat
     1261 gcagcgctac aggaccgaca aggaattcag caaaaaagtt ctcgccaaat acggcaagat
     1321 tcaggacgac gatatcctgg agggcacttg gcaggactac gctccgacct tgcagaaaac
     1381 gccgcggcct tcgctcaaag cggttcagtt tttgctcgac aatcaatttc ctgggaaaaa
     1441 actcaaggcg gagcagtttg tcgatttatc gttggtcgaa cagttggaga aaaacggctt
     1501 cttcgattcc gtaaccaagt gacggtcatc gatcccggag aataatatga aaattggctc
     1561 cagtagattt ttgcatttga gccttggtct attagctgtc ttgccgttcc ccagcgtggg
     1621 tgggtgcgca gctatcgact ccgttggtta ccgttcccag cgaaactgta aatctacaga
     1681 aacaccggtt attccgtgag gcgttcggca agggcttgaa tttggtccat cgcgcttttc
     1741 aacgccggcg cataaacccg cggcacgcac tcgccgcaaa tttctctcgc cagattgccg
     1801 atcgcgcgta aagcaaggtc gagattcgcc gattcgccgt tctcctccgg acaggagtcg
     1861 ctcaattcga ccaggctctg cagacagatc agcaaatttg ccaggatcga ttcgagcgtc
     1921 tcctcatccg gctcgatatt ggatcggtac aaaagatcat gaatggtctg cagctcgccg
     1981 cgaaaaaccc tgacggtgct ctccaatcgc cgcaggactt ggtgcggcag cgcgccctgc
     2041 tcgctctgga gctgttgcac ggagccaagg tcggaaaaaa ccaagtggag gttggtgcgc
     2101 acgcgatcga ggcgatcctc aaaggtcgtg cggtgaatct cggcagtgag ctcctcctgg
     2161 cgccgagata ctaattttga gatgacacaa ccaaagacca acaaccccgc gatcccttct
     2221 gccacagcga ggattcgcag cgggccgacc ggaattacat cgccataacc gatcgaaagc
     2281 gcggtgacaa agctaaagta gattgctgtg gcaagtccgc tggcatccgg gtgcacgacg
     2341 gagctgcccg cctgaagtcc ccaacccatg ccgatgccgg cgagccaata gatcacgccg
     2401 aagatcagaa tcatgcctag ccagaagccg accagccgct ccaacggcca gttcgcaacg
     2461 acttccaacc atgagaggcg atgggctgtc ttttgttggt gaacggagaa cgtttctggc
     2521 attgcgaccg acggcgtgtt gcactcgatc gatccaccgc tctctttcca atccgccaat
     2581 ccaccaacgt aatgacggac gttggtgtat cccaattttt ttagcaggcc gaccgcctgc
     2641 tggccgagcg gtcaggtggc tccgccgcag taaacggcga tttcctgatt taagttggca
     2701 atcagttggc gcgctttcgt ttcaatctcg gctagtggaa gactaaccgc actgggaata
     2761 tgcgcttcgg cgtaagctcc ctttggcaat acgtcgagga taatcagcga acgatctcgg
     2821 agccgcgcgc ggagctcgtc gcgcgagatc atctggtgct cgttagaagg ttctgttgat
     2881 agcagcttgg ccatgctagc ctgctcattc gagagaagca ggttcagcca ttttggatgc
     2941 ttcaaagcga aagtaagttg caagtctcat gcgctgcccc gacctccgtc gccacctgga
     3001 tcaccagcac cccacagctc gcaaagaata tatagccaac tctgtgttct tcgtgtttct
     3061 gctgcaaaat ctgccaagtc cgcactattc tgtagtacgc gactgaaacg gattggagtt
     3121 ttccatgcgc actgagctga tcagcatacc cacaccgacg catcctttag acgccgtcta
     3181 ctatacacct gacggtccgg gcaagggcgc ggtgatgtac tgccacggca accagatgaa
     3241 cttttacgtc tgcgccgccc ggtttctcgc gccgcatatc accaccctcg gctacgattt
     3301 catgcccttc aatcgtcggg gtcacgattc ggtcagcacc tacgatagcc gcgagtgcgt
     3361 cggcggcgct tatcaaacgg tcgccgaagg aatcgaagac aatgaaatcg cggcaaacta
     3421 tttggccgcc aggggcgaca attcgccaat tgtaatcggc cacagtaacg gcggggtatt
     3481 ggccagtgaa cacgtcgcgc ggcatccgca gacgcgcgcc ctgattctgc tctccgccca
     3541 cggcggtggt aatcgcctgg gcaatccaag ctcggcgcgc aatttttctc tcgccggcga
     3601 taccgctgcg ttaacgcgtc aagcagaagc gttggtgtcg gcgggcaaac cgcgccaact
     3661 gatgcttgtt ccagcgtggt ggtgggtaat ttccgcgcgc acttttctcg atcgattaac
     3721 caatgccccc gatttggtgg aaaatgccaa gcgcattaga tgccccgtgc ttttcatccg
     3781 cggcgatcag gagccgaagg aaaattatcc ggcggagcgc tttcaggaaa attgctccgg
     3841 cccgtgcgag gtggtgatcg tgccgaactg cgatcacttc tacgtcggtg ccgaagagaa
     3901 cgtcgcgaaa attgtcaccg aatggctaaa ccggaccttg ggagaatagt tgatgatttt
     3961 atgcgagacc agtgatgcag tgcacccgcc tcgatctgag atctgaca
//
LOCUS       DASULZ010000221         3505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1143145, whole genome shotgun sequence.
ACCESSION   DASULZ010000221 DASULZ010000000
VERSION     DASULZ010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143145"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1563)
                     /locus_tag="VFY96_04550"
     CDS             complement(<1..1563)
                     /locus_tag="VFY96_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5605762.1"
                     /translation="MHGGQAMLKAIERSGVEFILSSPGSEWPPVWEALAEAAARGAEQ
                     PKYINCRHEAVAVAMAAGYTKVTHRPQVVMLHATAGPLNAAMTLRAALHERTPMVILS
                     GEIAAYGENTKVADPGGQWLHDLTDLGGSPDLLRSCVKWADRISSAELLGSSIERAVR
                     IATEPPAGPVLIGIPFECMMEEVSFVDHGKPNEVVRAHEVNDGAIDKALSLIAAAKNP
                     VALTEHVGGDPRAFERFVELCELFSIPVMETYRPAFMNFPRSHPLYLHHDAKFVDAAD
                     LVLMVDAVTPWYPASKTPKNAKIICIADEYPNSRLPYWGYNVDLALVAPPAFTLEKLI
                     KKAQSSEQVAANREAYKQRLTSVRDRHNSYFEKLKDEARKHANETPIDPRWACHVLGE
                     ALPDNAVISEETTVYRNLIQETIPRDRPQSYFARVTGGLGVGLSYALGVKLALPDRPV
                     FALIGDGAFHYNAVPSCLGVAEEYGLPIHAVVFNNGRYLSMETSLIKYFPTGAAKQTG
                     VHFGANIGPRPDY"
     gene            complement(1585..2667)
                     /locus_tag="VFY96_04555"
     CDS             complement(1585..2667)
                     /locus_tag="VFY96_04555"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605763.1"
                     /translation="MMRTASNAEILHRRVLAGVLIGLIFLQSTASAAGPLLIAYGGFN
                     ETMAPLWIGIERGLFHKYGVDIAVLQTRSGQIMMTTLTTGGAALVWAAPSSAVSSSVA
                     GMKLGCFAAGNNKLPRELIVRKGIESVEDLRGKTFGVQSIGGGAWLSTMVALDALGLD
                     PDKYKLNVRVIGDTGTQTQAIVSGNIDAAVLPYSYGDIAKRAGARSLADAGALKLVYQ
                     ATVMCSLKSSPVVSTETMIALVKGLMESLLYILEPGNKRDVAAILKRNLRLSKDEDGE
                     AAARVAQSQMPNVEVGLNLDAWKTVRRLVARINPKVQEVDLDQLMMTSVTQNLEASGF
                     MAEMRKRVPPPPSSSPATRGRKAVGE"
     gene            2820..3386
                     /locus_tag="VFY96_04560"
     CDS             2820..3386
                     /locus_tag="VFY96_04560"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5605764.1"
                     /translation="MKRCALRVVLFAWVMSISGVSSAQDTIPFVPSPMHVVQRMLEVA
                     EITKGDVLYDMGSGDGRVVIEAAKKYGIRGVGVDLNPDLVAKARENAAKEGVSHLVEF
                     RAQDGLTVDISEASVVTLYMFKWFNNALRPKLQQLKPGARVIAHDFDIDEWKPTRIEY
                     VKSPEDGSDYAESRTLYIWIVGDKAPMP"
BASE COUNT          728 a          998 c         1030 g          749 t
ORIGIN      
        1 gtaatcgggt cttggtccga tgttggcgcc aaaatgaacg ccggtttgtt ttgccgcgcc
       61 cgtggggaaa tatttgatca agcttgtctc catcgataga taacgcccgt tgttgaacac
      121 gaccgcatga atgggcaagc cgtattcctc cgcgacaccc aaacatgacg gcaccgcatt
      181 ataatgaaat gccccatcgc cgatcagcgc aaatacggga cgatccggta aggcgagctt
      241 aactcccaac gcataactga gaccgacacc taagccgccg gtgaccctcg caaaatagga
      301 ctgtgggcgg tcacgcggaa tggtctcctg gatcaagttg cgatagacgg tggtttcttc
      361 cgagatcacg gcgttgtcgg gcagcgcttc accgaggacg tggcaagccc aacgcggatc
      421 gatcggcgtt tcattggcgt gctttcgcgc ctcgtccttc aatttctcga aatagctatt
      481 gtggcgatcg cgcacgcttg tcagtcgctg tttgtaagct tcccgattgg cggccacctg
      541 ctcggacgat tgggcctttt tgatgagctt ttcgagagtg aacgccggcg gcgcgaccaa
      601 agcgaggtcg acattgtagc cccagtaagg caggcgcgag ttggggtact cgtcggcgat
      661 gcaaatgatt ttcgcgttct tcggagtctt gctcgccgga taccaaggcg tgacggcgtc
      721 caccatcaaa accaaatccg ccgcatcgac gaatttggca tcatgatgca aataaagcgg
      781 atgactgcgc gggaagttca tgaacgccgg acgataggtt tccatcaccg ggatggaaaa
      841 cagctcacag agctcgacga aacgttcgaa ggcgcgcgga tcgccgccga cgtgttccgt
      901 gagcgccacg gggtttttgg ctgcggcaat gaggctcagt gccttgtcga tcgcgccatc
      961 gttcacctcg tgggcgcgca cgacctcgtt gggcttgccg tggtcgacga agctgacctc
     1021 ctccatcatg cattcgaagg gaatgccgat cagcacgggc ccggcaggcg gctcggtagc
     1081 aatccgcacg gcacgctcga tgctcgaacc gagcagttcg gccgaggaaa tgcggtcggc
     1141 ccatttcaca cagcttcgca gcaagtccgg cgagccgccg agatcggtta aatcgtgcag
     1201 ccactgaccg ccgggatcgg ccactttcgt gttctcgccg taggccgcga tctcaccgct
     1261 gagaatcacc atcggcgtac gctcgtgcag cgcggcgcgt aaagtcatcg cggcgttaag
     1321 cggtccggcg gtggcgtgca gcatcaccac ctgaggacga tgggtgactt tggtgtagcc
     1381 ggcggccata gcgacggcca cggcttcatg acgacaattg atatacttcg gttgctcagc
     1441 gccgcgcgcc gctgcttcgg cgagggcttc ccaaaccggc ggccattccg accccggcga
     1501 ggagaggatg aactcgacgc cgcttcgttc gatcgctttg agcattgctt gtccgccatg
     1561 catgggcact ctccttttgg ttggttattc ccccaccgct ttcctccccc gcgtcgcggg
     1621 ggaggatgaa ggagggggag gcacccgttt tctcatttcg gccataaatc cgctcgcttc
     1681 caaattctgc gtcaccgaag tcatcatcaa ttgatcgagg tcgacttctt gaactttggg
     1741 gttgatgcgc gcaaccaaac ggcggacggt cttccatgcg tcgaggttca gcccaacttc
     1801 gacgtttggc atctgtgatt gcgcgacacg cgccgccgcc tcaccatctt cgtctttgct
     1861 gagacggaga tttcttttga gtattgccgc gacgtcgcgt ttattgccag gctcgagaat
     1921 gtaaaggagc gactccatca gcccttttac gagcgcgatc atcgtttcgg tgctgacgac
     1981 gggagagctt ttcagagaac acatcacggt cgcctgataa accagcttca gagctccggc
     2041 gtccgccaag gagcgggcgc cggcgcgctt ggcaatatcg ccgtagctgt acggaagcac
     2101 cgcggcgtcg atgttgcccg agacaatggc ttgggtttgc gtgccggtat cgccgatgac
     2161 gcgcacattg agcttgtatt tgtccggatc aagaccgagc gcgtcgagag ccaccatcgt
     2221 cgatagccag gcgccgccgc cgatgctttg gacgccgaaa gtcttgccgc gcaaatcttc
     2281 gaccgattcg atgccttttc taacgatgag ctcgcgaggc agcttgttat tgccggcggc
     2341 aaaacaaccg agtttcatgc ccgccaccga ggaactgaca gcgctgctcg gggctgccca
     2401 gacgagagcg gcaccaccgg tcgtgagcgt cgtcatcatg atttgcccgc tgcgtgtctg
     2461 caagacggcg atatcgacgc cgtatttatg aaacaatcct cgttcgatgc caatccatag
     2521 gggcgccatg gtttcgttga agccgccata ggcgatcagg agcgggccgg cggcggatgc
     2581 cgtcgattgc agaaaaatca gcccgatcag aacgcccgca agaactctgc ggtgcaaaat
     2641 ttcggcgtta cttgccgttc gcatcataat tcctcggctg ctcctaccac accagcacgc
     2701 atttgcccag cccagagtga tcgccgcatg tgacgacgct tcaaaccatt gacaatccag
     2761 actgctggag agataagatt cgcgcgccat catttaatcc atcgatagga ggatatatcg
     2821 tgaaacgttg cgctctgcgt gtggttctgt tcgcgtgggt gatgtcgatt tcaggggtaa
     2881 gctcggcgca ggacactatt ccgttcgtgc cctcgccgat gcacgtggtg cagagaatgc
     2941 tcgaggtggc ggaaatcacg aagggcgatg tcctttacga catggggagc ggcgatggtc
     3001 gagttgttat cgaagcggcg aaaaaatacg gcatccgcgg cgtgggcgtc gatctgaacc
     3061 ccgatttggt cgccaaagcg cgcgagaacg cagcgaaaga aggcgtcagt cacctcgtgg
     3121 agtttcgcgc tcaagatggt cttaccgtgg atatttcgga ggcctcggtg gtgactcttt
     3181 acatgttcaa gtggttcaat aatgctttgc gtccgaagct gcagcaactc aaaccgggcg
     3241 cgcgagtgat cgcgcatgat ttcgacatcg acgaatggaa acccacccga atcgaatacg
     3301 tgaagtcccc cgaagatggc tcggattacg ccgagtcgcg tacgctctat atctggatcg
     3361 tcggcgataa agcgccgatg ccgtgaacga atttcttgga tcaccacaga ggcacggagg
     3421 ggactattca agcttcggga ataaacgacc tcaccgctaa gattatcggt gcagccattg
     3481 aagttcacaa agctcttggc ccagg
//
LOCUS       DASULZ010000222         3480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_939950, whole genome shotgun sequence.
ACCESSION   DASULZ010000222 DASULZ010000000
VERSION     DASULZ010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939950"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VFY96_04565"
     CDS             complement(<1..720)
                     /locus_tag="VFY96_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015858630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5605765.1"
                     /translation="MSFLRIQNLYKAFDRLVAVDRINLEIKQGEFFTLLGSSGCGKTT
                     TLRMIGGLEKPEGGEIHLGEQCLVSADQKIFVKPEKRDMGMVFQSYALWPHMTVFENV
                     AYPLKLRSASRSEIRGKVEEVLGLVGLSGLEDRPAPALSGGQQQRVALARALVFSPKV
                     LLLDEPLSNLDAQLREEMRRELKALQGRVNVTVIFVTHDQIEALSLSDRIAIMKFGRL
                     EQVGTSEEVYYYPATPFVRDFL"
     gene            complement(821..2557)
                     /locus_tag="VFY96_04570"
     CDS             complement(821..2557)
                     /locus_tag="VFY96_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX5605766.1"
                     /translation="MAKTETIKAALPARAWLPPLDGGTLIMAVLIGLMGFYVLYPLVL
                     ILINSFNTATIAEPPVYGLKAWREAFSEPGIWRSLWNSVKIGIVLQVVALPLGIFLSW
                     LISRTNILFANAFEFGFWISFFLPPLATTFGWMLLLDPSTGLINSWLRQLTGLSALNF
                     DIYSFSGIIWVHLVSHGISTKVMLLTPAFRRMDASMEEASRMSGAGPLTTLVRITVPA
                     MTPVIIVVFLLSVIRIFSSFEIELLLGVPWSFYVYSTKIVDLARQEPPLVNQAAALGS
                     VILLFLAAFIPLQRRLIARRQFTTVTGQFKPKIIDLGPWRYPATALVALVIFLLDVVP
                     VLSVLGGSFMTRFGFFNLPKTWTLDYWKMALSDPRILQGLQNTLIVAVSAALFGVLFF
                     SLVGYVLVRTKLPGRGILDSICWLPSAIPGVLSGLGLLWMFLGTPLFRPFYGTIVLLV
                     IASVLGGVTLSTQILKANFVQLGKELEEASRMSGAGFWKTYFRVVLPLMAQTMVMVGV
                     LKFMFASHHTSSIILLATSETRTLSLLALDQVAAGYREVASITVILIMALTLGMAVIS
                     RSLGLKVGIRAE"
     gene            complement(2765..>3480)
                     /locus_tag="VFY96_04575"
     CDS             complement(2765..>3480)
                     /locus_tag="VFY96_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5605767.1"
                     /translation="NASGSEAARYFAVTNCCFMMNLFHNSDYVFNSDYTFLDRFDPRT
                     EGYFSGQGELTGRFFLTTNFVPDTHTLKLTDYSERGKGSTNMKFNLAKNTMGAHISEF
                     PVGTYKKGHRHGPGAHVIILSGQGYSILWPQGEDIQRVDWKQGSVVVPPDQWFHQHFN
                     SGAKPARYLALRWNNWRYRFMRSQDGEGGTFTSVKLGGGQIEFEDEDPQIHRDFELAM
                     KLAGARCNMGTYHPRCTYK"
BASE COUNT          853 a          981 c          957 g          689 t
ORIGIN      
        1 gaggaaatcc cgcacgaacg gtgtggcggg ataataatat acctcctccg atgttccgac
       61 ctgctcgagc cggccaaatt tcataatcgc gatgcgatcg gacaaactga gtgcttcaat
      121 ctgatcgtgg gtgacaaaga tcacggtcac gttcacgcgg ccctggagcg ctttcagctc
      181 gcggcgcatc tcttcacgca attgcgcgtc taagttgctc agcggctcat cgagcaacaa
      241 caccttgggt gaaaacacca gggcgcgcgc cagcgcgacc ctttgctgtt ggccgccgct
      301 caacgccggc gccggacggt cttccaaccc ggacaagccc accaacccga gcacttcttc
      361 gactttgccg cgaatctcgg aacggctggc actacggagc ttcagcggat aggcgacgtt
      421 ttcgaaaacc gtcatgtgcg gccataaggc gtaggactga aaaaccatgc ccatgtcccg
      481 cttttccggt ttgacaaaga ttttttggtc cgccgaaact aggcattgct cgccgagatg
      541 gatttcgccg ccttccggct tttcgagacc gccgatcatg cgcagggtcg tcgttttgcc
      601 gcacccgctc gagccgagca aggtgaaaaa ctcgccttgc ttaatttcga gattgatgcg
      661 gtcaaccgcg accagtcggt cgaaagcttt gtaaagattc tgaatacgta aaaagctcac
      721 gcaatggcct gtcgtttgaa agcaggaggg aaaacggacg atagaagatc gacaaaccac
      781 catcttccat cttccattct cgaccgcata ccgaacatga tcactccgct cggataccga
      841 ccttgagacc caacgaacgc gagataacgg ccatgccgag cgtcagagcc ataatcaaaa
      901 tcaccgttat gcttgctact tcccgataac ccgctgccac ttggtcgagg gcgagcaagg
      961 acaacgtgcg agtctccgag gtggcgagca aaatgatgct cgaggtgtga tgcgatgcga
     1021 acatgaattt cagcacgccg accattacca tagtctgcgc catcaacggc aagaccactc
     1081 tgaaataggt tttccaaaaa cccgctccgg acatacgcga cgcctcctcg agctcttttc
     1141 cgagttggac gaagttcgcc ttgagaattt gggtcgagag tgtcacgccg cctaacaccg
     1201 aagcgatcac cagtaagacg atggtgccgt agaatggccg gaacaacggc gtccccaaaa
     1261 acatccaaag aagcccgagg cccgatagca caccgggaat cgccgaagga agccagcaga
     1321 tcgaatcgag aataccccgg ccgggcaact tcgtgcgcac cagcacgtaa ccgaccagag
     1381 aaaaaaacaa tactccgaag agcgccgcgc tgaccgcgac gatcaacgta ttttgcagcc
     1441 cctgaagaat gcgcgggtcg ctaagcgcca tcttccaata atcgagcgtc caggttttcg
     1501 gcaggttgaa aaatccgaag cgggtcataa agcttccgcc aagaacgctc agtactggca
     1561 ccacatcgag caagaaaatc actagtgcga ccagcgccgt tgccggataa cgccatggac
     1621 cgaggtcgat gatcttcggt ttgaattggc cggtgacggt cgtgaattgc cgccgggcaa
     1681 tgagccggcg ctgcagcgga ataaaggcag ccagaaatag taagattaca ctgcccagcg
     1741 ccgccgcctg attcaccaac ggaggctctt gacgcgccag gtcgacgatt ttcgtggagt
     1801 acacataaaa actccagggc acgccgagca aaagctcgat ctcaaagctg ctgaagatgc
     1861 ggatcacgct cagcagaaat accacgatga tgaccggagt catcgccggc acggtgattc
     1921 ggacgagggt ggtcagcggg cctgcgccag acatgcggct cgcttcttcc atcgatgcgt
     1981 ccatgcggcg aaacgccggt gtgagcagca tcaccttcgt ggaaatgccg tggctcacga
     2041 gatggaccca gatgattccc gagaaagaat agatgtcgaa gttgagcgcg ctcaaaccgg
     2101 tgagctggcg cagccatgaa ttgatcaatc ccgtgctcgg atcgagcaat aacatccagc
     2161 cgaaggtcgt ggcgagcggc ggcagaaaaa aagaaatcca gaagccgaac tcaaacgcat
     2221 tggcaaagag aatattggtg cgggaaatga gccaggacag aaaaatgccc aacggaagcg
     2281 caaccacctg tagaacgatg ccgatcttga cgctgttcca tagcgaacgc cagatccccg
     2341 gctcgctgaa ggcttcgcgc catgccttga gtccgtaaac cgggggttct gcgatcgtgg
     2401 ctgtattgaa gctgttgatc aggatcagca ccaaaggata aagaacgtaa aaccccatca
     2461 agccgatcag cacggccatg atcagggttc caccatccaa aggcggcaac caggcgcgcg
     2521 caggaagtgc agcctttatc gtttcggttt tcgccatcgt ttgctccccg gcataggcca
     2581 gggagtgtgt gtagcgcgtt gaggttgcga ggtcaagatt gaacagactg ttgagcaact
     2641 cgctcggggt tcgtgatgtt cagggggtcc ccataccgct tcaaatcatc gatccgcgcg
     2701 cgtgagactc ggaagtgtgc gagtcttttt tgcgccccgt aaggatcgcc atttcgaagc
     2761 cggctcactt gtatgtgcag cgaggatgat acgtacccat attgcaacgc gctccggcaa
     2821 gcttcatcgc caattcgaaa tcgcggtgga tttgtggatc ctcgtcctcg aactcgatct
     2881 gcccgccacc gagtttcacg ctcgtgaaag ttccgccctc accatcttgt gagcgcatga
     2941 aacgatagcg ccagttattc cagcgcaaag ccaggtagcg cgccggcttc gcgcctgaat
     3001 tgaaatgctg gtgaaaccac tgatccggcg gcaccaccac gctgccctgt ttccaatcga
     3061 cgcgctgtat gtcttcgcct tgcggccata gaattgaata gccttgcccg gaaaggatga
     3121 tcacatgcgc gccgggacca tggcgatggc ctttcttata agtgcccacg ggaaactcgg
     3181 agatgtgtgc ccccatggtg tttttagcca aattgaattt catgttggtg ctgcccttgc
     3241 cgcgctcgct gtaatcggtg agcttgagcg tgtgggtatc gggcacgaag ttggtcgtca
     3301 aaaaaaatcg cccggtgagt tcgccctgac cgctgaagta accttcggtg cgcggatcga
     3361 agcggtcgag aaaggtatag tcgctgttga aaacatagtc gctgttgtgg aagagattca
     3421 tcatgaagca gcaattcgta acggcaaagt aacgcgccgc ctcgctgccg ctcgcgttga
//
LOCUS       DASULZ010000223         2925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1244241, whole genome shotgun sequence.
ACCESSION   DASULZ010000223 DASULZ010000000
VERSION     DASULZ010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244241"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VFY96_04580"
     CDS             <1..374
                     /locus_tag="VFY96_04580"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEX5605768.1"
                     /translation="GDPWLFRFKNRVKEALCSGEQIVLPEASVSLAERFAVIVEHSEH
                     FERLCGRSRFMAMRKHLTWYCRNFRGAAEMRAKMTRANSADEVRCRLQEFAQNFSDAA
                     TMPTRQVPDQQWVRIDDNASV"
     gene            387..1010
                     /locus_tag="VFY96_04585"
     CDS             387..1010
                     /locus_tag="VFY96_04585"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX5605769.1"
                     /translation="MRLVLASTSPRRRQILALLDVPFEVIAPEFEERISAQLPADQEV
                     LEFALGKAQSVATKYPQGIVIGSDTIIELSGEKIGKPADREDARRILRGLSGKTHRIF
                     TSVALVDSGERSFMEVETVFVEMRRYTEEEIENYLGAGESADKAGAYSIQGHGSDLIK
                     SISGDYLAAVGMPLRRIALYLKSRGFPIADVEKVYAEKSFMNWRRFA"
     gene            1356..1547
                     /locus_tag="VFY96_04590"
     CDS             1356..1547
                     /locus_tag="VFY96_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605770.1"
                     /translation="MKQSNTYSNPDQNWHWPEKTWFRERLNAIKAREQETRQKVPTAN
                     ATTEPVDSSTTAKGDPKND"
     gene            1838..>2925
                     /locus_tag="VFY96_04595"
     CDS             1838..>2925
                     /locus_tag="VFY96_04595"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX5605771.1"
                     /translation="MAHAATEQSWDLEADVVIIGSGAAGLPAAVKAADGGASVIVVEA
                     NYDIGGHAIISGGNVPLGGGTSAQKKYGIKDSPDTVFSDLTDWSIVQPNGWPDYRYND
                     RAVMRAFADHCAPTFEFLLENGVKFKDIPPDNQGGHNIGNSAPRENHAFWTKGAGSES
                     PNNRAGTGVIRPLENSARAKGVRFLLSYKMASIVRAGEKTGRVIGVTADYNPRIMPGQ
                     SAPLKSFRSDGNIESTKSEMKIRAKKAVIVATGGMTSNVNFRRMFDPRLTDVLTVAGE
                     PYSYQDASGELAASAIGASLWGFANQTLENGDNIRTQRVLATKWNYMTWELECPIFPL
                     VRSTGLNVKDWHDLILVNQVGKRFYDET"
BASE COUNT          681 a          791 c          799 g          654 t
ORIGIN      
        1 agggcgatcc gtggctcttt cgatttaaaa accgcgttaa ggaagcactc tgctcgggtg
       61 agcaaattgt tttgcctgaa gccagcgttt cgctggcgga acgattcgcc gtaatcgtcg
      121 agcatagcga gcatttcgag cggctttgcg ggcgttcaag gtttatggcg atgcgcaaac
      181 atttgacctg gtactgccgg aattttcgcg gtgccgctga gatgcgcgca aaaatgacgc
      241 gtgccaactc ggccgatgaa gtgcgctgtc gattgcagga atttgcccaa aatttttccg
      301 atgccgcaac tatgccgacg cgccaagtcc ccgatcaaca gtgggtcagg atcgacgata
      361 acgcctcggt ctaaaagttc ccgttcatgc ggcttgttct tgcctcaacc tcaccgcgtc
      421 ggcgccaaat cttggctctg cttgacgtgc cattcgaagt catcgctcca gaattcgagg
      481 agcgaatctc cgctcaactt cctgccgatc aggaagtgct cgagtttgcc ctaggcaaag
      541 cgcaatctgt cgcaacaaaa tatccacaag gtattgttat cggtagtgac acgataatcg
      601 agctttcggg tgaaaagatc ggtaaacccg ccgatcgcga ggacgcgcgg cggattttgc
      661 gcggcctatc gggcaagacg catcgaattt ttaccagcgt cgcgctcgtc gattcgggcg
      721 aacgcagttt tatggaggtt gaaacagtat tcgtggaaat gcgccgttat acggaagagg
      781 agattgaaaa ttatctcggg gccggcgagt ccgccgacaa agccggcgcc tactccatcc
      841 aagggcatgg aagtgatctc atcaaatcca tttccggcga ttacctcgcc gcagtgggga
      901 tgcctctgcg ccgtattgct ctctacctaa agagccgcgg attccccatc gcggatgttg
      961 aaaaagtcta cgctgaaaaa tcgttcatga actggcggag gtttgcctga tcaatcatta
     1021 ctcgtaaccg tcggaaactt ctcgacatgc ttgtttttac ctttccgctg ctcgtacagt
     1081 agttgtgctt gagcaaagct gggcatctct gatcaaaata agccagaaac gcaaatcagc
     1141 acgccaagcc agcggcctct ctgtttcaaa gggcgaaagc gcatgaactc ttccttcgaa
     1201 gctgaccaat gcgcccaagc gcgtggatcc gtgcacgccg atttgtggct ggcgtgaacg
     1261 gatttgttcg aattctctca agcgaaattt ccgcagattc ttcagaactc tacaggttcg
     1321 cgtggtacag agcttgctct agctattgcc aaccgatgaa acaatcaaat acatattcca
     1381 atccggatca gaactggcat tggccggaaa aaacctggtt ccgcgaacgc ctgaatgcga
     1441 tcaaagctcg cgagcaggag acgcgccaga aagttccgac tgctaatgcc acaacggaac
     1501 cggttgactc gagcacgacc gccaaaggcg atccgaagaa cgattaagcg gtcgtttttc
     1561 cggcgctggt acccagcgcc accggccttt tcgagcccat ctcgtttctc ctcgtgcgat
     1621 gtcccgaccc atctcgattc atgtcagttc tgactctcct cacgtgattc aacagtactc
     1681 aatttagttg gccagtaaaa tctgctgcgg ccgctgtact ccttgctcag ctttgatcgc
     1741 gttcgatgga ttggctggcc tggctcttcg tagcttgcat ttttgtctat gcagatgtaa
     1801 tcgatttcag atttcacaga tcgcggagga tattgtgatg gcgcacgcgg caactgaaca
     1861 aagctgggat ctagaggccg atgtcgtgat aatcgggtca ggcgcagccg ggctgcctgc
     1921 ggcagttaaa gcggccgatg gcggcgcgtc cgtgattgtg gtcgaggcca actacgatat
     1981 cggtggccac gcgatcatca gcggtggcaa tgttcccctc ggcggcggca ccagcgcgca
     2041 aaagaagtac ggcatcaaag actcccccga cacggtcttt tccgatctta ccgactggag
     2101 cattgtgcag cccaacggct ggccagatta tcgctataac gatcgcgcgg tcatgcgcgc
     2161 gttcgcggat cattgcgcgc ccacattcga atttcttctg gagaatggtg ttaagttcaa
     2221 agatattccg ccggacaatc aaggtggcca caatatcggc aattcagcgc cgcgcgagaa
     2281 tcacgcgttc tggaccaaag gggccggctc cgaaagcccg aacaatcgcg ccggaacggg
     2341 agtcattcga ccattggaaa atagcgcacg cgcgaagggt gtgcgttttc tgctgagtta
     2401 caaaatggcg tcgatcgtgc gggcagggga gaaaaccggc agagtcatcg gtgtgacggc
     2461 ggactacaat ccgcgcatca tgcctgggca atccgcgccg ttaaagagtt ttcgctccga
     2521 cggcaatatc gagagcacca aatccgaaat gaagatccgt gccaaaaaag ccgtcatcgt
     2581 cgcgaccggc ggcatgacca gcaacgtaaa ttttcgccgg atgttcgatc cgcggttaac
     2641 ggacgtcctg acagtggccg gtgagcctta cagctaccaa gacgccagcg gcgaactcgc
     2701 cgctagcgcc atcggcgctt cgctttgggg tttcgccaat cagacgctgg aaaatggcga
     2761 taacatccgc acccaacgag tgctcgcaac gaagtggaat tacatgactt gggagctgga
     2821 atgtccgatc ttccccttag tgcgctcgac cggtttgaac gtcaaggatt ggcatgactt
     2881 gatcctggtc aatcaagtgg ggaaaaggtt ttacgatgag accaa
//
LOCUS       DASULZ010000224         2196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1244685, whole genome shotgun sequence.
ACCESSION   DASULZ010000224 DASULZ010000000
VERSION     DASULZ010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244685"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VFY96_04600"
     CDS             complement(<1..766)
                     /locus_tag="VFY96_04600"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5605772.1"
                     /translation="MQRLRNLRTLRSLKHRNFRLLLEGALVSSSGDFMQNVAQSWLVW
                     HVSRSPVAIGVVAFFDTLPRLLVGAVGGAIADRFDRRRVLMITQSLAMVQAIIYWFLV
                     QFDFIQLWHIAVLAFFLGIVNTINQTARQSLVNSLVPKEELLNAIGLQSSVFNFSKIL
                     GPSLGGVIIAWISIAGCFLVNALSFIYLIFNLYLMELPPWEKRADQKNIWADVKEGFH
                     YLLSNRRLLYIVGLSYVTATFVAPYNRFVPVFATNVL"
     gene            928..1446
                     /locus_tag="VFY96_04605"
     CDS             928..1446
                     /locus_tag="VFY96_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydratase"
                     /protein_id="HEX5605773.1"
                     /translation="MMQMPIVDLRSQARELVQTGRRVSVLWQYSDTLVFLARGREYRS
                     EFHINPSDETMQMVEGEMRLHYRTPDGKEAVAVIPEASIIYTPAGTPHSPRFSPDAFA
                     LISERKRRTGEIDRFHWYCAKCDQFLHEEKFIVHDYAEDPVSKAYKRFFDTEEFRTCK
                     KCGDVMPAPASL"
     gene            complement(1674..>2196)
                     /locus_tag="VFY96_04610"
     CDS             complement(1674..>2196)
                     /locus_tag="VFY96_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5605774.1"
                     /translation="GPQTIRRAHAVHPITAVQSEYSLWTRDYESDTIPTLKELDIGFV
                     SYYALGRGFFAGAVRDAAALGEKDSRRRSPRFSAENLPHNVRLLTELEKLASNKGITL
                     AQLSLAWILHRGENFAAIPGTLQIEHLEENFRAADVVLTAGDLATLDSLFPQKGAVAG
                     ARHEYDRSKELNI"
BASE COUNT          552 a          587 c          584 g          473 t
ORIGIN      
        1 gcagtacgtt ggttgcaaac accggcacga atcgattgta aggcgcaacg aacgtggcgg
       61 ttacatacga gagtccgacg atatacagca agcgcctatt gctcagcaga tagtgaaatc
      121 cttccttcac gtccgcccaa atgttctttt ggtcggcacg cttttcccag ggtggcaatt
      181 ccataaggta aagattaaaa ataagataaa taaagctaag agcattgact agaaaacatc
      241 cggcgatgct gatccacgca atgatcactc cacccagcga cggccccaga atcttcgaaa
      301 aattgaagac cgaagattgg aggccgatgg cgttgagtaa ctcttccttg ggcaccagac
      361 tattcaccaa cgactgacgc gcggtctggt tgatcgtgtt tacgatgccg aggaaaaagg
      421 ccagaacggc gatgtgccag agctggatga aatcgaattg gacaagaaac cagtagataa
      481 tcgcctgaac catcgctagc gattgagtga tcatcaacac tcgccgccga tcgaaacgat
      541 ctgcgatggc tccgccaacc gcgcctacca gaagccgcgg caaagtgtca aagaacgcca
      601 caaccccaat cgcgaccggg gagcgtgaga cgtgccagac caaccaactc tgcgccacgt
      661 tctgcatgaa atcgcccgag ctggaaacca gtgcgccttc taacaggagc cgaaagtttc
      721 gatgcttcag agatcggagg gttcgtaggt tgcggagacg ttgcatgttc gaacgctgcg
      781 acaaacgtct attcttacgc gactgcacca ggtttgcaaa gcacctcggg aatttttgct
      841 cgcgttgaat tgtaccgaat cagtcgaggg actaatatcg ttcagtcgag tgacactcgt
      901 tagacggtgc agcgtcggga ggcgcgcatg atgcaaatgc cgatcgtcga tttgcggtcg
      961 caagcgcgcg agttggtcca aaccggcagg cgcgtaagcg ttctttggca gtattctgat
     1021 accttggtgt ttcttgcccg cggtcgcgaa taccgtagcg agttccacat caatcccagc
     1081 gatgaaacca tgcagatggt cgaaggcgag atgcggttac attaccggac accggacggc
     1141 aaagaagccg tcgccgttat tcccgaagca tcgatcattt atacgccggc gggaactccc
     1201 cattcgccgc gcttttcacc cgacgcgttc gcgttgatct ccgagcgcaa gcggcggacc
     1261 ggcgagatcg atcggtttca ctggtactgc gcgaagtgcg atcaattttt gcacgaagaa
     1321 aaattcatcg tgcacgatta tgctgaagac ccggtctcga aggcttacaa acgctttttc
     1381 gatactgaag aattccgcac ctgcaaaaaa tgcggcgatg tcatgcctgc gccggcaagt
     1441 ttatgagtag cgcactgaac ctttataaga tttcagcaca caagatgtca gtaccggcaa
     1501 ttggtaaaac cgtcaccccc gaatgcttat cggaggtcca gatccgaaat ccctggattc
     1561 cgctaaaagc atgcgggaat gacagacggt aggcaatctt ttcaattaag ccagcaagcc
     1621 gcaggaaata attgacagag attggatcaa gcaccgacgc aaatattcat taattagata
     1681 ttcagttcct tcgaacggtc gtattcgtgc cgcgccccag ccaccgcgcc cttctgcggg
     1741 aatagactat cgagcgtcgc caagtcgccg gccgtgagga ctacgtccgc agctcggaaa
     1801 ttttcctcga ggtgctcgat ttgcagcgtg ccgggtatcg ccgcgaaatt ctcgccccga
     1861 tgaagaatcc aagccaacga tagctgcgcg agcgtaattc ctttgttgct cgcgagcttt
     1921 tcgagttcag tcaataggcg tacgttgtgc ggcaaatttt cagcggagaa gcgcggcgat
     1981 cttcgccggc tatccttttc cccaagcgct gctgcatctc gcaccgctcc ggcaaagaaa
     2041 ccgcgtccaa gcgcatagta cgatacaaag ccgatgtcga gttctttgag tgtgggaatt
     2101 gtgtcgctct cgtaatcgcg agtccaaagg gaatattcgc tctgcacagc ggtgatcggg
     2161 tgaaccgcat gcgcgcggcg aatcgtttgc ggtccg
//
LOCUS       DASULZ010000225         2271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_229667, whole genome shotgun sequence.
ACCESSION   DASULZ010000225 DASULZ010000000
VERSION     DASULZ010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229667"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..>2271
                     /locus_tag="VFY96_04615"
     CDS             230..>2271
                     /locus_tag="VFY96_04615"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEX5605775.1"
                     /translation="MIFKCLLCILVAPFVSQAVSPISLDGVWRFDLDPGDVGVAQQWF
                     NRPMRGFIRLPGSLQSQNGGNDITTDTPWVLSLYDRYWHLREEYKAYTERGKVKVPFL
                     SQPMRHYLGAAWYQREIEIPRNMQGRRILLTLERPHWQTTVWVDERLIGSDKSLVAPH
                     VYDLGLLAPGRHRLTIRVDNRMQMPYRPDAHSVSDSLGASWNGIAGKIQLEDTGRVWI
                     DDVQVIPNLARHSMLIRVKIGNATGRSGQATLTAIWPDTGIGPATWDENGGSAEVEVP
                     LRSDVETWDEFHPKLLPLKLWLRGNDVEEYKDVAVGLRDFHAEGNQFILNGRQINFRG
                     THFGGDFPLTGYPPTDVKSWRQLFELCKKWGLNHMRFHSWCPPEAAFEAADQVGFYLQ
                     PEPGMWNEISPGTPMERMLYEETDRMIRAYGNHPSFMLLSASNEPKGKWQESLSKWVE
                     NYRARDPRRLYASGTGHTERQLENITEGTDYLAIQRINQKMLRRESGWFGSDFADSLK
                     EINIPVISHEVGQWAAYPDYDIINDFKGYLRPGNFEIFRDSMKAHGLLEKNKAFADAS
                     GKFQLACYKEEIEANLRTPGLGGFQLLDIHDYLGQGTALVGLLDAFWRPKSYVKADEF
                     TEFCNSTVPLARLTKRVFTNSERLVTDVELAHFGAEPLDKVKAVWRIDESFAKGEW"
BASE COUNT          523 a          620 c          640 g          488 t
ORIGIN      
        1 ctgatcggcg gcttcgtccc gccagagata tttgacgaac cggaattttt cttctgggcc
       61 ttgagaagtc attatgctaa aaagagccac caaaatgcgc gtaactttac tggataaaac
      121 tcggttacgt ctagcgttct ctctgcggcc ctctgcgtac ctctgcgctc tctgcgttaa
      181 acggctattt cagcgcagag ggcgcagagg tacgcagagg gccgcagaga tgattttcaa
      241 atgcctgttg tgcatcctgg tcgcgccctt cgtttcgcaa gcggttagtc cgatttcact
      301 tgatggcgtc tggcggtttg accttgaccc gggcgatgtc ggagtggcgc agcaatggtt
      361 caaccggcca atgcgcggct ttattcgtct acccggaagt cttcagtcac agaacggcgg
      421 taacgacatt acgaccgata cgccgtgggt gctctcgctc tacgatcgtt actggcacct
      481 gcgcgaagag tacaaagctt acaccgagcg cggcaaagtc aaagtgccgt ttttgtcgca
      541 gccgatgcgt cactatcttg gcgcagcctg gtatcagcgc gaaattgaaa ttccgcgaaa
      601 catgcagggc cgccgcattc tactcacgct cgaacggccg cattggcaaa ccaccgtctg
      661 ggtcgatgag cgattaatcg gttcagacaa gagcctggtt gcgccacacg tttacgatct
      721 tggcctgctc gcgccaggcc gtcatcgcct gacgattcgt gtagacaatc ggatgcagat
      781 gccctaccgg ccggacgcgc acagcgtttc cgattcactc ggcgcaagct ggaacggaat
      841 cgccggcaag atccagctcg aagacacggg gcgcgtgtgg attgatgacg tgcaagtcat
      901 tccgaacctc gcgcggcatt cgatgttaat ccgagtgaag attggtaacg ccaccggtcg
      961 cagcgggcaa gcaacgctga ctgcgatctg gccggatacc ggcatcgggc ccgcaacgtg
     1021 ggatgaaaac ggtggcagcg ctgaagtgga agttccactc cgcagtgacg tggaaacgtg
     1081 ggatgagttt catccaaaac tgctgccgtt aaaactctgg ctgagaggaa acgacgtcga
     1141 agaatacaaa gacgtggccg ttggactgcg ggattttcac gcggagggaa atcagttcat
     1201 tctcaatggc cgtcagatca attttcgcgg cacacacttc ggcggggatt ttccgttgac
     1261 gggttacccg ccgactgacg tcaaatcgtg gcggcaatta ttcgagctct gcaaaaaatg
     1321 gggcctcaat cacatgcgct ttcattcgtg gtgcccgccc gaagcagcct tcgaagccgc
     1381 cgatcaagtc gggttctatc tgcaacccga acccggaatg tggaatgaga tcagcccggg
     1441 cacgccgatg gagcgcatgc tttatgaaga gaccgatcga atgattcgcg cctacggcaa
     1501 tcatccttcg ttcatgctcc tgtcggcgag caacgagccg aaaggcaaat ggcaggagtc
     1561 tctgtcgaaa tgggttgaga attaccgcgc gcgcgatcca cgccggcttt acgcgagcgg
     1621 caccggccac accgaacggc aactcgaaaa cattaccgaa ggcactgatt acctcgcgat
     1681 acaacgcatc aatcagaaga tgttgcggcg tgaatccggt tggtttggct ccgacttcgc
     1741 tgactcgctc aaagagatca acattccggt tatctcccat gaggtgggcc agtgggctgc
     1801 gtatcccgac tacgacatca tcaacgactt caaaggctat cttcgccccg gcaacttcga
     1861 gatctttcgc gactcgatga aagcgcacgg actgctcgaa aagaacaaag cattcgctga
     1921 cgcgtccgga aaatttcaat tggcatgcta caaggaggag attgaagcaa acttgcgcac
     1981 gccgggactc ggcggatttc agctcctcga tattcacgat tacctcggac aaggaacagc
     2041 gctcgtcggc ttgcttgatg ctttttggcg tccgaagagt tatgtgaagg ctgatgagtt
     2101 cacagagttc tgcaattcaa ctgtcccgct cgcgcggctc acaaagcgcg tttttaccaa
     2161 tagcgaaaga ttagtcacgg acgtggagct ggcccatttc ggcgccgagc cgctcgacaa
     2221 agtcaaagct gtctggcgca tcgatgagag ctttgcgaag ggagaatggg a
//
LOCUS       DASULZ010000226         3242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1346943, whole genome shotgun sequence.
ACCESSION   DASULZ010000226 DASULZ010000000
VERSION     DASULZ010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346943"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            243..1202
                     /locus_tag="VFY96_04620"
     CDS             243..1202
                     /locus_tag="VFY96_04620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5605776.1"
                     /translation="MSSLVDTSENRTSALETPSLLPSLSIIIPTRNRAGSLKLLLESL
                     AEPSRRGMPETEIIVVDNGSTDETRRWLDAQREQNQSGRLIILNEPRPGKSNAINRAL
                     VVARGEILMVLDDDVVVESNCLAKHAEAHAQDQFAAIQGRILPGKDFAGNPADANRLR
                     EYNIPLIDYGEEIVAIRGLTGTNMSFKRVVFDKIGFFDPRLGPGAAGFSEDTEYSIRI
                     RKAGFKIGYTPHAIVYHELNPSRSGRVYNRAVEYRKGVSRSLYRRDSILTKVLPDLFA
                     NCVRYAIYRLLGRHDKIYKTEGRILKCWGYLAGSLGRGARRVV"
     gene            1214..2116
                     /locus_tag="VFY96_04625"
     CDS             1214..2116
                     /locus_tag="VFY96_04625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5605777.1"
                     /translation="MLSRLPAVSVIVATYNRAKLLRETIDSILQQRFQDYELIVVDDG
                     STDNTRAMLEGLGSRIRYFYQENRGPSAARNAGVRHAVGRWISIQDSDDLCAPNHLEC
                     LYGYVKDHPHYGMVFANGGYLGGSKHDRETIIPTAKSRRLANRGVQLADLFDKSIVRL
                     QAALISKAAYEAAGGHDENLRICMDLDLAFRLFMNSPVSYVDEVVFFYRKHEGNSSRN
                     EELRLTENIRVIEKLLAQNKTAGALLGEKRVAARLAYRYYRLAKGRRKHGQRSEARSA
                     IRQASRLRPLAVKYQLYRLRWAFF"
     gene            2326..3138
                     /locus_tag="VFY96_04630"
     CDS             2326..3138
                     /locus_tag="VFY96_04630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5605778.1"
                     /translation="MRIDPLSSREQTPAAVSAIVVCFNEEDRIEACLRSLAWCDEIVV
                     VDSYSTDRTPEICRSYTDRFIQREWAGYRNQKAFAHSQATKEWVLLVDSDERVTEELR
                     NEIVEALARDGGRYCGYAVSRLVRYLERWWRRGGWYPDYDVRLFRRDRAAWGGSDPHE
                     KILVDGPVRRLRHPLEHYSYRSIEDHLQRINRFTSISARELKKDGGRWRLSAALLHPA
                     GRFFRSYFLKRGFMEGFAGFYVAVTAAVYVFLKYAKLWELELEEKKRLGRKR"
BASE COUNT          728 a          805 c          972 g          737 t
ORIGIN      
        1 gaaagagtac cttggaaaca gctttgccga gcgaagaatc aggcgccagt ggattaaaaa
       61 aatcatgcgc caatccgagc ggttgaatcg cgccaaagcc gcttctgggt aatgatgtta
      121 ctatctattg cacgggggaa agaaaccgaa ctcgtatagc aagtgaatcg tgaagatctt
      181 tgaaagcagg gcgatgtgtc ggtggagagg aacgaggtaa atggcggtgc cggcagactt
      241 ggatgagctc gctagttgat acgagcgaga accgtacctc agcgttggag actccatcgc
      301 tgctgccgtc gttgagcatc attattccga cgcgaaatcg cgctggaagc ttaaagctct
      361 tactggagag cctcgccgag ccgtcccggc gagggatgcc ggaaactgaa atcatcgtcg
      421 tggataacgg ctcgacagac gaaacccgcc gctggctcga tgcgcaacgc gagcagaacc
      481 agagcggacg gctaattatc ttaaatgagc cacggccagg aaagtccaac gcaattaatc
      541 gagctctcgt agttgcgcgg ggtgagattt tgatggttct cgacgacgat gtcgtcgttg
      601 aatcgaactg tctggcgaag cacgctgaag cgcatgcgca agatcaattc gccgcgattc
      661 aaggtcggat tctcccgggc aaggacttcg ccggaaatcc agcggacgcc aatcggctgc
      721 gagaatacaa tatcccgcta atcgactacg gcgaggaaat tgttgcgatc cgcgggttga
      781 ccggcactaa catgtcattc aaacgagtgg tgttcgataa gatcgggttt ttcgatcctc
      841 gtctcggccc gggggcggca ggtttcagcg aagacacgga gtattcgatc cgcattcgta
      901 aggctgggtt caaaatcggc tatacgcccc atgcgattgt ctatcatgag ctcaatccgt
      961 cgcgttccgg ccgtgtttac aatcgcgctg tggagtatcg aaaaggcgtc agccgaagcc
     1021 tttaccggcg cgactcgatt ttgacgaaag tcttgcccga cttgttcgca aactgcgtcc
     1081 ggtatgcaat ttatcggctg ctcggacggc atgacaaaat ctataaaacc gaaggacgga
     1141 ttttaaagtg ttggggttat ctggctggta gtctcggccg cggtgcgcgg cgggtggttt
     1201 aaggggagat cgtgtgctga gccgattgcc tgccgtctcg gtgattgtgg cgacttataa
     1261 tcgcgcaaaa ttgctccgcg agacgatcga cagcatattg caacaacgtt ttcaggacta
     1321 tgagttgatc gtcgttgacg atggatcgac agacaacacg cgcgcaatgc tcgagggctt
     1381 gggctcacgt atacggtact tctaccagga aaataggggc ccctctgcag cccgcaatgc
     1441 cggagtgcgt cacgcggtgg gacgatggat ttcgatacag gactccgacg atttgtgtgc
     1501 cccgaaccac cttgaatgcc tttacgggta cgtcaaagat catccgcatt acgggatggt
     1561 tttcgcgaac gggggctatc tcggcggctc gaagcacgac cgggaaacga ttattccaac
     1621 ggcaaaatcg cgccggttgg cgaatcgcgg agtgcagctt gccgatctgt tcgacaagag
     1681 catcgtgcgc ctgcaagccg cgctcatatc gaaggctgca tatgaagcgg cgggcggcca
     1741 tgatgaaaat ttgcgcattt gcatggatct cgatttggct ttccgactat tcatgaattc
     1801 tccggtttcg tatgtggacg aggtggtctt cttttaccgt aagcacgagg gtaacagcag
     1861 ccgtaacgaa gagcttcggc tcacagaaaa catccgggtg atcgagaagc tgctggctca
     1921 aaacaaaact gccggggcat tgctcggcga aaagcgcgtg gcagccagat tggcttatcg
     1981 gtactaccgc ttggcgaagg gccggcgcaa gcatggacag cgcagcgaag cgcgcagcgc
     2041 tataagacaa gcctctcggt tgcgaccatt ggcggtgaaa tatcaactct atcgtcttcg
     2101 ctgggcattt ttttagcccg gtgagatggc gctaaaattt agcatcatca tcgcaacatt
     2161 tacccgtcgt gaatctctgg ctcggctgcg gcgggtcgca aagtattagc cggtgcggaa
     2221 attccttgaa aaaatgaagc gatcatgtag cagccccccg gtcagctaaa ccagggcgtc
     2281 ggccgagttc aggttctcac cgccttacgc cctccgtttg taacgatgcg catagaccca
     2341 ctatcaagcc gcgagcaaac gcctgccgcc gtttccgcga ttgtcgtttg tttcaatgag
     2401 gaggatagga tcgaagcctg ccttcgaagt ctcgcttggt gcgacgagat cgttgtggtg
     2461 gattcttata gtacggatcg cactccggag atttgccgca gttacaccga tcgattcatt
     2521 cagcgcgaat gggctggtta ccgaaatcaa aaggctttcg cacattcgca agcgacgaag
     2581 gaatgggtgt tgctcgtgga ttcggacgag cgagttaccg aggagttacg caatgagatc
     2641 gttgaggcat tggcgcgcga tggtggccgg tattgcggtt atgcggtgtc gcggctggtt
     2701 cgatatttgg agcgctggtg gcggcgcgga ggctggtatc ctgattacga cgttcgcctg
     2761 tttcgacgcg atcgagcggc atggggcggc tcggacccgc acgagaaaat tctcgtcgat
     2821 ggccccgtgc gccggctgcg gcatccgctg gaacattatt cgtatcgcag catcgaagat
     2881 catctgcagc gcatcaaccg cttcacttcg atctcggcgc gggagttaaa gaaagacggc
     2941 ggccggtggc ggcttagcgc ggcacttctg catccggccg gtcggttctt ccgttcatat
     3001 tttttaaagc ggggtttcat ggaaggcttt gccggttttt atgttgcggt gacggccgcc
     3061 gtttatgttt ttctcaaata tgctaagctc tgggagcttg agttagagga gaaaaaaaga
     3121 cttggtcgga agcggtagtc gccgcctcct caacattctg catgtcgatc ccgagaaaaa
     3181 ctggggtggt ggggaagcgc aggtgtttgg tctactggct tatctgtcag ccagaggtca
     3241 tc
//
LOCUS       DASULZ010000227         2330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_535189, whole genome shotgun sequence.
ACCESSION   DASULZ010000227 DASULZ010000000
VERSION     DASULZ010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535189"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VFY96_04635"
     CDS             complement(<1..623)
                     /locus_tag="VFY96_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX5605779.1"
                     /translation="MDWLVDPQVWIAFVTLVALEIVLGVDNIVFISILAGKLPAGQQQ
                     RARNIGLSLALVMRVILLFSLSWVVALTRPLFTILGQEISGRDLILLLGGLFLLAKAT
                     FEIHENLEGEEGRASAKVAPTFTGVIVQILLLDAVFSLDSVITAIGMVDQVEIMIAAV
                     IVAIAFMMVFAGPVAHFVQRHPTVKMLALSFLLLIGLTLLVEAFDVH"
     gene            928..2247
                     /locus_tag="VFY96_04640"
     CDS             928..2247
                     /locus_tag="VFY96_04640"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX5605780.1"
                     /translation="MASIINRGTSCWDVASAEQTGLLIDGRNYYRAFYQAARAAKRYI
                     LICGWQFDSDVMLLRGKDAADGDEVCLLSFLNRLCEKNENLQIYILAWNFNAVYGIDR
                     EWFQKWYFNWTTNDRLTFCFDDCHCLGASHHQKFAVIDGTIAFLGGLDLCSSRWDDRD
                     HCADNPQRVNADQNSYTPFHDVQSYHVGAVAEKVAELFKHRWKLACCKDLDLPETTEK
                     QRIDFPGSLPIVSSRVGISRTQAKTEDGRQSSHQEIRRLFVSAIESAENLIYLENQYF
                     SSNALFRALSRRMTDSGRPLLEVVMILAQDANAFFEKISIGLMQAKLIARLKELASKT
                     GHKIGVYYPACLGTDGQEVPTYIHSKLFLIDDRFLSVGSANMNNRSMGLDTEVNVAWE
                     AAPEEHAMIESIRRVRVDLLAEHTGTTDSASLGELTASKGLVDYLER"
BASE COUNT          572 a          617 c          626 g          515 t
ORIGIN      
        1 atgtgcacgt cgaaagcctc gacaagcaag gtgagcccga tcagcaacag aaagctcaaa
       61 gccaacattt tgaccgtggg atggcgttga acaaagtgtg ccacgggccc ggcgaagacc
      121 atcataaagg cgatcgccac gatcaccgcc gctatcatga tctcgacctg gtcgaccatg
      181 cctatggcgg taatcaccga atctaacgag aacaccgcgt ccaacagcag aatctgcaca
      241 atcacgccgg taaatgtcgg tgccactttc gcagacgcgc ggccttcctc gccttcgaga
      301 ttttcgtgaa tctcaaacgt agctttggct agcagaaaca acccgccgag cagcaaaatc
      361 agatcgcgtc cggaaatttc ctggcccaaa atagtgaaga gcggtcgagt cagcgcgacc
      421 acccacgaga gggaaaagag cagaataacc cgcatcacca gcgccaaact cagaccgatg
      481 ttgcgcgcgc gctgctgttg ccccgcgggc aatttcccag ccaggatcga aataaatacg
      541 atattgtcga cgccgaggac gatctccaaa gcgaccagag ttacgaaagc gatccatact
      601 tgtgggtcga cgagccagtc catagattta catccttcct acgcagtcga tctcgtgtcc
      661 gcatgctcta gtcatggctg cccaccgcct gccttgggcg cgcttgtcag cgaaccccgt
      721 caaagaaact tgaatgcgtc cgtgcaccgc tcgcctttga tcacatggct gtgggcgaag
      781 ccaaagtatg ctcaatgaga gtgctaatga aaatggctag tcgtcgtata agattgtctg
      841 gttgcaggat ctctccctag catctcatgg catcgcagcg actcggattt tgggctacgc
      901 tatgaaaggt cagtgagcga ggtcacgatg gcatcaatta taaatagagg aacaagctgc
      961 tgggatgtcg cttccgcaga gcaaactggc ctgctgatcg acggtcgcaa ctactaccgg
     1021 gctttttatc aggccgcgcg ggcggcgaaa cgctacattt tgatttgcgg ctggcaattc
     1081 gacagcgacg tcatgttact gcgtggaaag gatgccgcag acggcgatga ggtgtgcttg
     1141 ctctcgttct tgaaccggct ttgcgagaag aatgaaaatc tgcagatcta cattctcgcc
     1201 tggaatttca atgcggtcta cggaatcgat cgcgaatggt ttcagaaatg gtatttcaac
     1261 tggaccacca acgaccggct cacgttctgc tttgatgatt gtcattgttt gggagccagc
     1321 caccatcaaa aattcgccgt gatagacggc acgattgcat tcttgggcgg attggacctg
     1381 tgctcgagtc gttgggatga tcgcgatcat tgcgctgata atcctcaaag ggtcaatgcg
     1441 gatcagaatt cctacacgcc gtttcatgac gtgcagtcct atcatgtcgg agccgtcgcc
     1501 gaaaaagtgg cggagctatt taaacatcgc tggaagcttg cctgctgtaa ggacctcgat
     1561 ctacccgaaa caacagaaaa acagcggatt gattttccag gaagcttacc gattgtgtcc
     1621 tcccgtgtcg gaatcagccg gacacaggca aaaacagagg acggcagaca gagttctcac
     1681 caagaaattc gtcggctttt tgtctccgcc atcgaaagtg ccgaaaacct gatttatctc
     1741 gagaatcaat acttcagctc gaacgctctc tttcgagcgc tgtcgaggcg aatgacggat
     1801 tcgggtcggc ccttgcttga ggtcgtgatg attttggccc aggatgcgaa cgcgtttttc
     1861 gagaaaatct caatcggttt gatgcaggcc aaactgattg ctcgtttaaa ggagctcgcc
     1921 tcgaaaacgg gccacaagat aggagtttac tatccggcct gtttaggcac ggatggtcag
     1981 gaggtgccca cctatattca ctcgaagctc ttcctgatcg acgatcgctt cttgagcgtg
     2041 ggttcggcga acatgaacaa tcggagcatg ggactcgaca ccgaggtcaa cgtagcctgg
     2101 gaggccgcgc ccgaggaaca tgccatgatc gaatccatcc ggcgtgtccg agtcgatctt
     2161 ttagccgaac acacgggtac aacggattcc gcgagcctcg gtgagcttac cgccagtaaa
     2221 ggcctggttg attatctgga gcgctgagca gggagcggct caagccggct tcgccgtcac
     2281 ccgtgtttgg atgttgaccc ggcagaatat gaatctttga catcgatgct
//
LOCUS       DASULZ010000228         3329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_28321, whole genome shotgun sequence.
ACCESSION   DASULZ010000228 DASULZ010000000
VERSION     DASULZ010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28321"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VFY96_04645"
     CDS             <1..792
                     /locus_tag="VFY96_04645"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX5605781.1"
                     /translation="GKLLAGGHSLIPAMKLRLQSPRVLIDLGTVAGLRGIRLDNNELV
                     IGALTVHSDIAASDLVRKYLPGLAEAASLIGDLQVRNRGTIGGSVAHADPGADFPVIL
                     SALKAAFVVQSAADTRTISVDDFFMDFFTTAMSTNEILTEIHVPLPPADSGSGYAKFP
                     HPASGYVVVSAGALITRQASGTCTASRIAIGGLASGPLRAIATEMELQGKPLTPQLIA
                     SAAAKAADESDPVDDPYASAEYKKHVATVYVRKAVEAAAARAATN"
     gene            982..3297
                     /locus_tag="VFY96_04650"
     CDS             982..3297
                     /locus_tag="VFY96_04650"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5605782.1"
                     /translation="MTTEHTSNGLIGASLKRVEDPPLITGRGCYVDDINLPGMLHLAF
                     QRSPYPHAKIVAIDISKAKAMPGVEAVISGNDISEKLNLTSSQLLPNMKTPPHPVLAR
                     GAVHCIGVPVAAVVAKTRAQAEDAANAVDVEYQPLPSIANAEEALKPGGSLAREEIDS
                     NICYTLTKNGGNVDKAFAAADHIFTMHIASPRQVALAMEPRGILASPDPFGKTLSVWL
                     STQGPHRARAEIANVLGFPEHKIHLIAPDVGGGFGSKGPVYREDIVACYLALQLRKPI
                     KWVATRSEDFVTTIQGRDQAMTSEMALRKDGKILGLKVKVVANLGAYLHASTAGPPQR
                     MLGMACGCYQIQDVRVEIVAVFTNTCPTGPYRGAGRPESVLNIERLLDKAAAVLDMDR
                     LEIRRKNFIRPDQFPYTTGTGVEYDSGDYEKPLREALQMSNYHDLLRQRDERRKRGEL
                     VGVGFSTFVEPSGGAGFESGTVRVERTGEITVLTGSSSHGQGHETSWAQIAAELMKTA
                     PENVTILHGDTHVSQQGTGTFGSRSAVVGGGALAIAAERVIEKAKRIAAHLVEAAPED
                     IVQAAGGFAVKGVPEKKISWRQIAAAAYGGRIPKGMELGLQETVFFDPKREAWGFGTH
                     VAFVSIERDTGKLTIEKLVLVDDCGMVINPMIVEGQIHGGVAQGLGEAQCEQMLYDAD
                     GQVQTGTFMNYAIMRAADMPPMVLGETITPNPFNPIGVKGVGECGTNGAPPSVANAVM
                     DALSPLGIEHIDMPYTAPKLWQAIRTAEHRN"
BASE COUNT          728 a          966 c          983 g          652 t
ORIGIN      
        1 ggcaaactgc tcgccggcgg tcatagtttg attccggcaa tgaagctgcg tcttcagtcg
       61 ccgcgcgttt tgatcgatct cgggaccgtc gctggcctgc gcggtattcg actggacaat
      121 aacgagcttg tcatcggcgc gcttaccgtg cacagcgata ttgcagcctc ggatctcgta
      181 cgtaagtact tgcccggatt ggccgaggct gcgtcgctca ttggcgacct gcaagtgcgc
      241 aaccgcggca cgatcggcgg gagcgttgct catgccgatc caggagctga ttttccggtg
      301 attctcagtg cgctgaaagc cgccttcgtc gtgcaatcgg cggcggacac tcgcacaatt
      361 tccgtggacg atttcttcat ggattttttc accaccgcga tgagcacgaa tgagattctt
      421 accgagattc atgtgccgct tccgcctgcg gatagcggca gcggctatgc gaaattcccg
      481 catcccgcat cgggctacgt cgtcgtgagc gctggagctt tgataacccg gcaggcttcc
      541 ggcacgtgca ccgcctcgcg aatcgcgatc ggcggtctcg ctagcgggcc gctccgcgct
      601 attgccacgg aaatggagct gcaaggaaaa ccgctgacgc cacagctgat cgccagtgcc
      661 gctgcgaagg cggcggatga aagcgatcct gtggatgatc cctatgccag cgcggaatac
      721 aaaaagcatg ttgccacagt gtacgtacgc aaagcggtcg aagcggctgc ggctcgtgcc
      781 gccacgaatt aacggtattt gttgaattcc ctctcccgtt gagagtgtgt cgcaatggta
      841 aaaacacgcg catgtcattc tgaacctcag ggcgaagaat ctggttttac cgctgcgaca
      901 cggtctcgtt cggagagaca agagtgagga ccctcacctc aatcctctcc caaaggaaga
      961 ggaactaaga gaggtaaaac catgaccacc gagcacacat ccaatggact gattggcgcc
     1021 tcattgaagc gcgtcgaaga tccgccgctc attaccggca gaggctgtta cgtcgacgac
     1081 atcaacttac cgggtatgtt gcacttggct tttcagcgca gtccctaccc tcacgcaaaa
     1141 attgtcgcta tcgacatcag caaagccaaa gccatgcccg gagtcgaagc cgtcatcagc
     1201 ggcaatgaca tcagcgaaaa gctcaatctt acgtcgtccc agctgctgcc caacatgaag
     1261 actccgcccc atccagtatt ggcgcgcggt gcggttcatt gtattggcgt gcccgtcgct
     1321 gcagtggtcg caaagacacg cgctcaggcg gaagacgcgg cgaacgcggt cgatgtcgaa
     1381 taccaaccgc tgccatcgat cgccaacgcc gaggaggcgc taaagcctgg cggatcgctc
     1441 gcgcgcgaag agatcgacag caatatttgt tacacgctga ccaagaacgg cggcaacgtc
     1501 gacaaggcct ttgccgcggc agatcatatt tttaccatgc acattgcgag tccgcgtcaa
     1561 gtcgcgctgg cgatggaacc gcgcggcatc ctagcgagcc cggatccctt cggcaaaacc
     1621 ctgtcggttt ggctttctac ccaaggaccg catcgcgcac gcgccgagat cgcgaatgtg
     1681 ttgggattcc ccgagcacaa aatccatctg attgcgcctg atgttggcgg cggcttcggc
     1741 agcaaaggtc cggtctatcg cgaagacata gtcgcctgct atctcgcgct gcaactccga
     1801 aaaccgatca aatgggtcgc gacccgcagc gaggattttg tcacgaccat tcagggccgc
     1861 gatcaggcga tgacgtccga aatggcgctt agaaaagacg gcaagattct cggcctgaaa
     1921 gtgaaagtcg tcgctaatct cggcgcgtat ctgcatgcga gcacggccgg accgccgcag
     1981 cgtatgctgg gaatggcatg cggctgctat cagatccagg atgtccgggt cgaaattgtc
     2041 gcggttttca ccaacacctg cccgacgggt ccgtatcggg gcgctggtcg gccggaatcg
     2101 gtcttaaata tcgagcggct gctcgacaaa gccgccgcgg ttttagacat ggatcgccta
     2161 gagattcgcc gcaagaattt tattcgcccc gaccagttcc cgtacacaac gggaaccggc
     2221 gtggagtacg attccggcga ctatgaaaag ccgctccggg aagcactgca gatgtccaat
     2281 taccatgatc tgctgcgcca gcgtgacgaa cggcgcaagc gcggtgaact ggtcggcgtt
     2341 ggcttctcca cttttgtcga gccgagcggc ggcgccggtt tcgagagcgg cactgttcgc
     2401 gtcgagcgca ccggtgagat caccgtgctc accgggtcga gctcccatgg gcaggggcat
     2461 gagacttcct gggcacagat tgctgccgag ctcatgaaaa ccgctccgga gaatgtgacc
     2521 attctgcatg gcgacaccca cgtgagccag caaggcaccg ggacatttgg cagccggagc
     2581 gcggtagtcg gcggcggcgc tttggccatt gcagccgagc gcgtaattga gaaagcaaaa
     2641 cgcattgcgg cacacctcgt cgaggccgcg ccggaagata ttgttcaagc cgccggcggg
     2701 ttcgctgtca agggtgttcc ggaaaagaaa atttcttggc gtcaaatcgc cgcggcggcg
     2761 tacggcggca gaattcccaa aggaatggaa ctcggtcttc aggaaacggt tttcttcgat
     2821 ccgaaacgtg aagcttgggg gttcggcacc cacgtggcgt ttgtcagtat cgagcgagat
     2881 accggcaaac tgacgatcga gaagcttgtg ctggtcgacg actgcgggat ggtgatcaat
     2941 ccgatgatcg tggaagggca gattcatggt ggagtcgccc agggacttgg cgaggcacaa
     3001 tgcgaacaga tgctctatga cgccgacggc caggttcaga ccgggacgtt catgaactat
     3061 gcgatcatgc gtgccgccga catgccgccg atggttttgg gcgagaccat cacaccgaat
     3121 ccgttcaatc ctatcggtgt caaaggcgtc ggcgaatgcg gcacgaatgg cgcgccgccc
     3181 tctgtggcca atgcggttat ggacgcgctt tcaccacttg gcatcgaaca tatcgacatg
     3241 ccttacacag cgcccaaatt atggcaggcg atccgcacag ccgaacaccg aaactaagcc
     3301 gacaatcgca ccataaatag cccgcgacg
//
LOCUS       DASULZ010000229         4819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1447817, whole genome shotgun sequence.
ACCESSION   DASULZ010000229 DASULZ010000000
VERSION     DASULZ010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447817"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1758)
                     /locus_tag="VFY96_04655"
     CDS             complement(19..1758)
                     /locus_tag="VFY96_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX5605783.1"
                     /translation="MEAVLRMPRAISLRLRTSPVLLGLAILPMLVIVILVSVLVWISF
                     QTGIVGTPNASYTLRNYVEILGEPFVLKVLWNTIVFTLATTATALVLGLPIAWLTERT
                     SLPGKGLVYTIMTLGLLIPGIYTAMGWTFIAHVRIGLLNRWLTDTFGLAEGPINIATP
                     VGMGFVQGLNLTPLAFILTAQMFRAMNPALEEAAKIHGMSFLGTARRITLPLAFPAIL
                     AATIYMLTIGIATFDVPAIIGLGNRIYMLSTYVYLRANPPDSGIPQYGITAALGTLMI
                     GVALLLTWWYSQVLRQDHRYQVITGKGYRPQLIQLGNWAILAWVFIALYFVASKLTPL
                     LLIAYAAFVPYLAPPSYEMLGKMTLAGFYNLNWDLFWRGLHNTLLLVVTVPLVTLLLA
                     FSISWLIVRSRNRWRYALEFGAFLPHALPEVILAIGALLLALFVLQGIVPLYGSVWLI
                     AIIYVISRLAFATRALNSSLLQIHKELEEAAYTAGLSPVRTSWRILVPLLRPTLMSVW
                     IWSAILVYRELTVAVFLISQDSVTLPAVIWSYWFSGAINQAAAVTLLMTLALTPLILI
                     FWWFGRRQIVPES"
     gene            complement(1842..3062)
                     /locus_tag="VFY96_04660"
     CDS             complement(1842..3062)
                     /locus_tag="VFY96_04660"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5605784.1"
                     /translation="MISIRKLAKEFIVSKGKVAALAHLDLEVAEGDFFVIVGASGSGK
                     TTLLRCVAGLETPDGGEICISGRPVFRDHPPIWTPPQERNLGMVFQSYAVWPHLTVFE
                     NVALPLREGAQKIPRREVTSRVRQALYMVQLEEQADRSATLLSGGQQQRVALARAIAV
                     NPRVLLMDEPLSNLDARLREQVRGKIRELAKQLGATVLYVTHDQIEAMAIADKIALMS
                     FGKLLQYGNPMELYRRPNCAEVADFFGSVNWLSGTLIAPTLVHTKIGRFEVSAHSVTG
                     PDVLVGFRPEALQVVEQTEICVQNSFEASLRSSIFLGDQFVYETVSEDLLLIGKSRQI
                     PLCPQQCLRLRVEPSDIMVFPCSEKRESVMTTNYLSPEIARLTDEMQSASAAQGSSDL
                     NPPLGRRRHGAKLF"
     gene            complement(4194..>4819)
                     /locus_tag="VFY96_04665"
     CDS             complement(4194..>4819)
                     /locus_tag="VFY96_04665"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5605785.1"
                     /translation="ALDQLQFALENGACGIFMRPLEGTRQITDPYFFPLYEKAQKIGF
                     CIGLHQSNGSAAAVDMMINPDGSRDFFNQYRIFNVGALFRIINCGIPELFPRLRFGFI
                     ETAASWVPWVIYELRRRHDTVDRKLPDDLLERCGIFVTCQIGDDVPYLIRTCMGENTL
                     MIGTDYGHADSSTELDALTTLKSSGGISAAQHKKIVEDNPRKFYGFA"
BASE COUNT         1212 a         1348 c         1295 g          964 t
ORIGIN      
        1 aagaggtggg gactcaactt acgactccgg cacgatctgg cgccgcccaa accaccagaa
       61 aataaggatg agcggcgtga gcgccagggt catcaaaagc gtaaccgccg ccgcctgatt
      121 aatcgccccg gaaaaccaat aactccagat gaccgcgggg agggtcacgc tatcctggct
      181 gatgagaaaa acagcgacag tcaattcccg ataaaccaga atcgcggacc agatccagac
      241 cgacatgagc gtagggcgaa gcaagggaac gagaatacgc catgaggtcc ggacaggcga
      301 aagtcccgcg gtataggccg cctcttccag ctccttgtgt atttgaagca gagagctgtt
      361 gagcgcccgg gtagcgaatg ccaggcgaga gatgacgtag atgatggcaa taagccatac
      421 cgatccgtaa agaggcacaa tcccctggag cacgaacaaa gccagcaaca acgcgccgat
      481 ggcgagaatg acttcgggca gcgcgtgcgg cagaaacgcc ccaaactcca aagcgtaacg
      541 ccaccgattg cgtgaacgaa cgatcagcca tgagatgctg aaagcgagaa gcaaggtcac
      601 cagcggcacg gtcacaacca ggagcagagt gttgtgaagg ccgcgccaga acaggtccca
      661 gttgagatta taaaaacccg ccaatgtcat tttaccgagc atttcgtagg acggtggagc
      721 cagataagga acgaaagccg catacgcgat cagcaagagt ggagtcaatt tcgaggcaac
      781 aaaatacagc gcgataaaaa cccaagccaa tatcgcccaa tttccgagct gaatgagctg
      841 gggacgataa cccttgccag taataacctg atagcggtga tcttgccgga ggacctggct
      901 ataccaccag gtcagcagga gcgctacgcc aatcatgagc gtccccaagg ccgcggtgat
      961 gccatactgc gggattccgc tgtccggcgg attggccctt aaataaacgt aggtgctaag
     1021 catgtaaatc cgattgccga gtccgatgat ggcgggcacg tcgaaggttg caatcccgat
     1081 ggtcagcata tagatggtgg ccgccaatat ggccgggaat gccaagggca acgtgatacg
     1141 gcgcgcggtg cccagaaaac tcatcccgtg aatctttgcc gcctcttcga gggcggggtt
     1201 catagcgcga aacatctgag cggtcagaat aaaagccaag ggcgtcaaat tgaggccttg
     1261 cacgaatccc attccaaccg gcgtcgcaat gttgatcggc ccttcggcga ggccaaaagt
     1321 atccgtcaac catcgattca gcagtccgat ccgcacgtgc gcgataaagg tccagcccat
     1381 ggcggtatag attccgggaa taagaagccc aagggtcatg atcgtgtaga ccagtccctt
     1441 gccaggcaag ctggttcgct cagtcagcca agcaatcgga agtcccaaaa caagtgccgt
     1501 cgctgttgtc gccaaggtga agacgatggt attccataac actttgagca cgaacggctc
     1561 gcccaggatc tccacgtaat tgcgtaatgt gtagctcgcg ttcggcgtgc caacaattcc
     1621 ggtttgaaaa ctgatccaca ccagaaccga gaccaaaatc acgatcacca gcatcggcaa
     1681 tatcgccagt cccagaagca caggggaagt gcgcagccgc agggaaatcg cgcgcggcat
     1741 tctaagtacg gcttccataa taagtactcc tgcgtttaac ccggcgggaa gactttacct
     1801 ctttccttcg atcggtgcgg gatcgactat tactgtatca gctaaaataa tttcgcccca
     1861 tgacggcgtc tcccaagcgg tgggtttaaa tccgaactcc cttgagctgc gcttgcggat
     1921 tgcatctcat cggtcaggcg cgcaatctct gggctgaggt aattagtcgt cataaccgat
     1981 tctcttttct ccgaacaagg aaaaaccatg atatcggaag gttccacgcg caaccgcagg
     2041 cactgctgag ggcagagtgg gatctgacgg ctcttgccga tcagcagcag gtcttcagaa
     2101 acggtctcgt acacgaactg gtcgcccaga aagatactag agcgaagact cgcttcgaaa
     2161 gaattctgaa cacagatctc tgtctgctcg acaacctgca atgcttccgg cctgaaaccg
     2221 accaaaacat ccgggcccgt aacgctatgc gccgatacct cgaaccgacc aatttttgta
     2281 tggactagcg tgggagcgat caacgtaccg gacagccaat tgaccgatcc gaaaaaatca
     2341 gccacttcgg cgcaattggg acggcgataa agctccatgg gattgccgta ctgaagcagt
     2401 ttgccgaagc tcatcagggc aatcttgtcg gcgatggcca tggcttcgat ctgatcgtgc
     2461 gttacataga gaactgtggc gccgagttgc ttggccagtt cgcggatctt gccgcgcact
     2521 tgctctctga gccgcgcatc aagattgctt aagggctcat ccatcagcaa aacgcgcgga
     2581 ttgaccgcaa tggcccgcgc caatgcgacc cgctgttgct ggccgccgct cagcaaggtc
     2641 gccgatcgat ccgcttgctc ctcaagctgc accatgtaca gcgcctgcct gacccggctg
     2701 gtaacttcac ggcggggaat tttctgtgcg ccttcccgca gcggtaaagc gacgttttcg
     2761 aaaaccgtca aatggggcca tacagcgtac gactgaaaga ccattccgag attgcgctcc
     2821 tggggcggtg tccaaatcgg cggatgatcg cgaaataccg ggcggccgga aatacagatc
     2881 tcgccgccgt cgggggtttc aagtccggcc acgcagcgca gcaaggtggt cttgcccgag
     2941 ccgctcgctc cgacgatcac gaagaaatcg ccttcggcca cctccagatc gagatgggca
     3001 agcgctgcta ctttgccttt actaacaatg aactctttgg cgagcttacg gatggagatc
     3061 atgacgactt gaccgactca tttgcctcgg ccggacactt gattcgcaaa ccgcccgaga
     3121 acgatctgcc cggctgcctt agctccgttc cggcggtagg cgcctgttgc gctctccccc
     3181 tgcgagagga ctggggtgat gatgcacttt gcgcctcgaa ggctccagcc gtttccaaac
     3241 accagggtgc cggccaaggc ataactaccg actcgcttaa cttgagtcaa atgattcttc
     3301 cccgtgctat cacccccaga gcgccgggac atctgacaat ctcaatccag acaaagagac
     3361 cgtcgatttc ctgttcagga aagtgcgaga catttgtcgt cagcttttta ccgatgatct
     3421 ttgaaccgtc tttcaccctg caccagatac ctgactgtgg gtgtggaact caagaaaagc
     3481 aaagatctcg caaacccaga attatatccg agagagacat gtcgagatgg tgtgcgttgt
     3541 tgatctcaaa actggtgagt ttcacgttac cgggagatcg tatcctacga tagaaaacgc
     3601 aaagggttac gacgagagcc aatactggga cgacgcgaaa acaatactgg gcaactgcaa
     3661 cgagaggaag acgcagaact gggacggggc gaagaacctg aggcgcttga ggttctggat
     3721 cgcctcacag tatgcctcat tcaacgtgga cgaagtcgcg aagcctgcga aagagtgaat
     3781 tcatattttt caatttatcc tcgatcgtgg ccgcggcatg gcaaagcgga tcgtgaagcg
     3841 cattgaaaag ttactcgagg cgagtttcgc ggtgagccct gacacctaat tcgcgatgga
     3901 acctcgcgca ccgtaccggg gaaaagaggc aaaggtttta cattcgcagc cagtctcgat
     3961 ctgccgcgcc cgcctttact gctgaaaatc ccggggtcgt ccattaccag cgggaatcaa
     4021 gttgtctgtc aagcaactct ttggcaagga ttcataacgc gccagcagct caggcagctc
     4081 tacaaaaatg aaagggcgac ccaagtcgcc ccttcatttt tgtgttccct ccgctcttgg
     4141 cgctgaatca ctcgttgcag attctcgctc tactcctctc cgttagtgcg gccctacgcg
     4201 aacccgtaaa atttcctcgg gttatcttca acgatctttt tgtgctgcgc ggcagagatg
     4261 ccgccgctgc ttttcaaagt cgtcagcgcg tccaactccg tagaagaatc ggcgtgacca
     4321 taatcggtgc cgatcatcag cgtattttcg cccatgcagg ttcggatcag atagggcaca
     4381 tcgtcaccga tctgacaggt gacgaaaatt ccgcagcgct cgagcagatc gtccggcagt
     4441 ttgcgatcga ccgtatcatg gcgccggcgc agctcataga tcacccacgg cacccaggaa
     4501 gccgccgttt cgatgaatcc aaagcgaagt cgcggaaata actccgggat gccgcaattg
     4561 ataatccgaa acagcgctcc gacattaaaa atccggtact ggttgaagaa atcccgactg
     4621 ccgtcgggat tgatcatcat gtccaccgcc gcagcgctgc cgttggactg atgcagtcca
     4681 atgcagaaac ctattttttg ggctttttcg tagagcggaa aaaaataggg atcggtgatc
     4741 tgccgtgttc cttccagcgg cctcataaaa atcccgcacg cgccgttttc cagagcaaac
     4801 tgcagctgat ccagcgcat
//
LOCUS       DASULZ010000230         2538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1245257, whole genome shotgun sequence.
ACCESSION   DASULZ010000230 DASULZ010000000
VERSION     DASULZ010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245257"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /gene="sthA"
                     /locus_tag="VFY96_04670"
     CDS             complement(<1..1094)
                     /gene="sthA"
                     /locus_tag="VFY96_04670"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HEX5605786.1"
                     /translation="MAHYDMLVIGSGPAGQKAAIQAAKIGKKVGVIERKKVAGGICIN
                     TGTIPSKSLREAVLFLSGFRQRNLYGASYRVKKDITFEDLAQRCDHVIKAEQEVIQNQ
                     LLRNSVDFIIGTASFLDPHRLSIRQESETNEHTADYIVIAVGSEPARPAEIPFDDESI
                     IDSDGLLSLKQLPKSLTIVGGGVIGCEYASILATLGIPVVLVERRPRLLEFVDSELIE
                     ALQYQMRNVGVTFRFNEEVVGIQKSADHSVSIQLKSGKKISAPLLMYSIGRIGATKDL
                     NLNTIGLMPDERGRLKVNENYQTALAHVYAVGDVVGFPALASTSMQQGRHAACHAFGL
                     DCDLSTHLLPYGIFTIPEISMVGRNEDELTR"
     gene            complement(1117..2067)
                     /locus_tag="VFY96_04675"
     CDS             complement(1117..2067)
                     /locus_tag="VFY96_04675"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX5605787.1"
                     /translation="MLFLKDTPISQILSMHEMIGAIEETLKEVALGRGHELPRRRIHH
                     PNRMIFGILPGSVHGVMGAYLQTDLDRRIHRETVILYSVETAAPLILFQDCAINENRT
                     AAAGAIGAKWLARKNASRAAVFGSQVHAETQLKALAAVRKLTSARVFSPNAARRAAFA
                     EKLGRELQIAVLPASSAEEALVTADIVITATNSTAPVFDGRLLQEGTHITSIANGDKT
                     RIRQEIDELTLRRAAPVFITSKTTVCANESDIFRAVRDGLISWEQVHEISHLLLGKAS
                     GRTDERQITLFKLQGTGIMDVAIGLCAYEKIKNGNLAQQL"
     gene            complement(2191..>2538)
                     /locus_tag="VFY96_04680"
     CDS             complement(2191..>2538)
                     /locus_tag="VFY96_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX5605788.1"
                     /translation="EIVRSGVQSVARGQGEAAIATGLTRNQTMRLVILPQALRNMIPA
                     LVTQFIVLLKDTSLASIIGYVDLTKAAQIVNNREIRPFELYLFIAVVYWVCSYGMSQL
                     ARYCERQLNPALS"
BASE COUNT          570 a          683 c          700 g          585 t
ORIGIN      
        1 cgggttaatt catcttcgtt tcttccgacc atggaaatct ccggaatcgt aaaaatgcca
       61 tagggcagca ggtgtgtcga gagatcacaa tccaggccga acgcatggca ggcggcatgg
      121 cgtccttgct gcatagaagt cgaagccagc gcaggaaagc ctactacgtc accgacggcg
      181 tacacatgag cgagcgcggt ctgatagttc tcatttactt tgaggcgtcc acgctcatcg
      241 ggcatgagac cgatagtatt taggttgaga tccttggtcg cgccgatgcg gccgatcgag
      301 tacatcaaaa gcggcgcact gatttttttt ccgctcttca gctggatcga taccgaatga
      361 tcggcagatt tttggatacc gaccacttcc tcgttaaagc gaaaagtgac gccgacattg
      421 cgcatttgat attgcagagc ttcgattagc tcgctatcga caaattcgag cagacgcggc
      481 cgtcgctcga ccaagacgac cggaattccc aacgtcgcga ggatcgaagc gtactcgcaa
      541 ccgatcacac cgccacccac gatggtcaag gatttgggta attgtttaag agacaacaat
      601 ccgtcactat cgatgatgga ctcgtcgtcg aagggaattt cagccggacg agcgggctcg
      661 ctgccgacgg caataacaat ataatcggcg gtgtgctcgt tggtctcgga ttcctgcctg
      721 atcgaaagcc ggtgtggatc gagaaatgac gccgtgccga tgataaaatc gacagaattc
      781 cgcagcaact ggttttgaat cacttcctgt tcggctttga tcacgtgatc gcatcgttga
      841 gcgagatcct cgaaggtaat gtctttcttg acgcggtagc tggcgccgta aaggttgcgc
      901 tggcgaaagc ccgataaaaa caagaccgcc tcgcgcagcg atttgctcgg gatcgttccc
      961 gtgttgatgc agatcccgcc agcgaccttc ttgcgttcaa tgacgccgac ctttttgccg
     1021 attttggcgg cttggattgc tgccttttgt ccggccggcc cggagccaat caccaacatg
     1081 tcgtaatgag ccatcgttgc gtcctggatt gtgctgctaa agctgttgcg cgagattgcc
     1141 gttcttgatc ttttcgtagg cgcaaaggcc aatggcgaca tccataatgc ccgtcccttg
     1201 gagcttaaac agcgtaatct gccgttcatc cgtgcgcccg gaagcctttc cgagaagcaa
     1261 atgcgagatc tcgtgcacct gttcccagga gatgagtcca tctctgacgg cacgaaaaat
     1321 gtcgctctcg ttggcgcaga ccgtcgtctt cgacgtaata aaaacaggcg cggctcggcg
     1381 cagcgtgagt tcgtcaatct cctgacggat tcgggtcttg tcgccgttgg cgatcgatgt
     1441 aatgtgagta ccctcctgaa gcaatcttcc gtcaaatacc ggcgcggtgg aattggttgc
     1501 cgtaatcacg atatcggccg ttaccaaagc ttcctcagcg ctagaagcgg gtaggacggc
     1561 gatctgaagc tcacggccga gtttttccgc aaaggccgct cggcgtgcgg cgttcggact
     1621 aaaaacccgc gcgcttgtca attttctaac cgcggcgagc gccttgagtt gggtttcggc
     1681 atgaacctgg ctcccgaaaa cggccgcgcg gctggcattt ttccgggcca accatttggc
     1741 accgattgcc cctgctgcgg cggttcgatt ttcattgatc gcacaatctt gaaagagaat
     1801 aagcggcgca gcggtctcga cgctgtacaa aatcacagtt tcgcgatgaa tccggcggtc
     1861 gagatcggtt tgcaaataag cgcccatcac gccgtgcacc gagcctggca agattccgaa
     1921 aatcatccga ttggggtgat gaatacgccg ccgtggaagc tcatgaccac ggccgagagc
     1981 aacttcttta agagtttcct cgatcgcgcc aatcatctcg tgcatcgaaa gaatttggct
     2041 gatcggcgtg tctttcaaga acagcatgtc tctgcaggca gaattgacgg gtctccacga
     2101 tgctagcaat gggcgctcac ctcggcaacc ctggaagcga gcggcgctga ttaaattttt
     2161 atctcgcggc tgtaatgggg gtcgagtttg ttatgacagc gccgggttta gttggcgttc
     2221 gcaataacgg gccaactgag acatgccgta actacacacc cagtacacca ccgctataaa
     2281 gagataaagc tcgaacggac gaatttcgcg attgttaacg atctgcgccg ctttggtgag
     2341 gtcgacgtag ccgataatgg atgccagtga ggtgtctttg agcagcacga taaattgcgt
     2401 caccagtgcg ggaatcatat tgcgcaaggc ttgcggcaaa atcacaagcc gcatcgtctg
     2461 attgcgcgtc agaccggtcg caatcgccgc ctcgccttga ccgcgcgcca ccgattgtac
     2521 gccggagcgg acgatttc
//
LOCUS       DASULZ010000231         2510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_434190, whole genome shotgun sequence.
ACCESSION   DASULZ010000231 DASULZ010000000
VERSION     DASULZ010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434190"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1268)
                     /locus_tag="VFY96_04685"
     CDS             complement(45..1268)
                     /locus_tag="VFY96_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEX5605789.1"
                     /translation="MPKRFHFFTTGISLLVLLGSARAQGALPEGTGKTAVQTYCVQCH
                     DLSTVTRSGYDAAGWRNNLHMMINVGSSLPQSEVEPLVQYLSKNFPARTRPPAKVISG
                     NVKVSITEWMVPTPGSRPHDPLATVDGAIWYTGQFANTLGRLDPKSGKIKEFHLPDKS
                     GPHGLTTDKDGNIWYTGNFKATIGKLNPKTGEVTEYFMPNPSARDPHTPIFDKNGTLW
                     FSVQGGNMVGRLKPETAEIKLVSAPTPKSRPYGMVISSQNIPFFAEFGSNKIASIDPD
                     TMAIRDYTLPHADSRPRRVAITSDDVIWYSDYSRGYLGRYDPATGKASEWASPGGPQS
                     QPYGIIAVNNVIWYSEAGVAPNTMVRFDPKTEKFQTWKIPSGGGVVRNVSLTKDGNIA
                     IACSGVNQIGLVKLN"
     gene            1444..2169
                     /locus_tag="VFY96_04690"
     CDS             1444..2169
                     /locus_tag="VFY96_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5605790.1"
                     /translation="MAEPVLMTREGDVVTILMNRPESGNRQTDATWAKVTEMFDAGSR
                     ESRLIIFKGAGDDFCLGREAMGQATPVLEAYAVRERSETIFNLYGAFRNARVPIIGVV
                     QGRAVGLGCALAALCDITIASDRARFQFPETAHRIMPTIAFSALIDRMPRKAATFMMY
                     SAQEIDAQKALTFGIASDVVASNNLDGAVSTLVEHLKKMPLPAVLALKEYARSAFGMS
                     THGATDFARNLHATVNSFSGMKA"
     gene            complement(2259..2336)
                     /locus_tag="VFY96_04695"
                     /pseudo
     CDS             complement(2259..2336)
                     /locus_tag="VFY96_04695"
                     /inference="COORDINATES: protein motif:HMM:NF022815.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2305..2463)
                     /locus_tag="VFY96_04700"
                     /pseudo
     CDS             complement(2305..2463)
                     /locus_tag="VFY96_04700"
                     /inference="COORDINATES: protein motif:HMM:NF022815.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
BASE COUNT          533 a          704 c          684 g          589 t
ORIGIN      
        1 agacgaagtc atcgacgatt gtaaaaaact tcactgtcac caaactagtt caacttcacc
       61 aatccgattt gattgacgcc gctgcacgcg atcgcgatgt tgccgtcctt cgtcaaactc
      121 acgttccgca cgacgccgcc gcccgatgga attttccagg tctgaaattt ttccgttttg
      181 ggatcgaaac gtaccatcgt atttggcgcc acgccggctt cgctatacca gatcacatta
      241 ttgacggcga tgatgccata gggttgcgat tgcgggccgc cgggcgatgc ccattcgctc
      301 gcctttcctg tggctggatc atagcgccca agataaccgc gggagtaatc ggaataccag
      361 atcacgtcgt cactggtaat cgcgacacgc cgcggccggc tgtcggcatg cggcagcgtg
      421 tagtcgcgga tcgccattgt atcgggatcg atgcttgcga tcttgtttga gccgaactcg
      481 gcaaagaatg gaatattctg cgaactgatc accatgccgt agggccgcga ctttggcgtc
      541 ggcgcagaca cgagcttgat ttctgcggtt tccggtttga gtctcccgac catatttcca
      601 ccttgcacgc tgaaccagag agtaccgttc ttatcgaaga tcggcgtatg cggatcacgc
      661 gccgacggat tgggcataaa atattccgtg acctcgccgg ttttcggatt gagctttccg
      721 atcgtggcct tgaagtttcc cgtgtaccaa atgttgccgt ctttatccgt ggtcaaaccg
      781 tgcggtccgg atttatccgg taaatgaaat tcctttatct tgccgctctt tggatcgagc
      841 cgtcccagag tgttggcaaa ctgccccgtg taccagatcg ctccgtctac cgtcgcaagt
      901 ggatcgtgcg gccgtgatcc cggtgtgggc accatccatt cggttatgct gactttcaca
      961 tttccggaaa tcaccttggc cggaggtctc gttcgcgcgg gaaagttttt cgataaatac
     1021 tgcaccagcg gttcgacttc gctctgcggt aaactcgagc cgacgttgat catcatatga
     1081 agattattgc gccagcccgc ggcatcgtaa ccgctgcgcg tgacggtgct gagatcatgg
     1141 cattgcacgc agtaggtttg caccgccgtt ttgccagtcc cttcagggag cgcgccctga
     1201 gcgcgcgccg acccaagcaa caccaagaga gatattcccg ttgtaaaaaa gtggaatcgt
     1261 ttcggcaaga agaacctcct ctggatcgtc cgcattctat tgccgggcgc gcagcttcgc
     1321 aagaataagc acgagaattg aaatcgattt gtcgattacg gtcgagccat gcgcgccggc
     1381 gttgacaacc cgagcgaggc tcctctacag tcctcgcagc ctcatcagaa ggagagagat
     1441 cgcatggcag aaccagtact gatgacaaga gaaggcgatg tcgtaacgat tctcatgaat
     1501 cgcccggaaa gcggtaaccg ccaaacggat gcgacctggg caaaagtaac ggagatgttc
     1561 gatgccggat cgcgggaatc ccggctgatt atcttcaagg gagccggtga cgacttttgc
     1621 cttggccgcg aagctatggg acaagccacg cccgtgctcg aggcctatgc tgtgcgcgag
     1681 cgcagtgaaa cgattttcaa tctatacggc gcttttcgca acgcccgcgt gccgatcatc
     1741 ggcgtcgttc aggggcgcgc cgtcggcctc ggctgcgctc ttgcagcgct ttgtgacatt
     1801 acgatcgcga gcgacagagc gcgttttcaa ttccctgaaa ccgcccatcg catcatgccg
     1861 acgattgcct tttccgcatt gattgatcgg atgccgcgca aagccgctac attcatgatg
     1921 tattcagccc aggaaatcga tgcgcagaaa gctcttacgt ttggcatcgc gagcgatgtc
     1981 gtagcaagca ataatctcga tggcgcagta tcgacgctcg ttgagcactt gaagaaaatg
     2041 ccgctgccgg ccgtcctcgc acttaaagaa tacgcccgca gcgcatttgg tatgagcacg
     2101 cacggcgcca ctgattttgc ccggaaccta catgcgacgg tgaacagttt ttcaggaatg
     2161 aaagcctagt agcccggtgg cgtcgccttt tctcgactta gtgaccgatt ttcgcggttc
     2221 tacggtgata tagacgacgc aattcttcct tggcttttct aagcggaccc ggcgagagtc
     2281 ggataattta ccaccacccg cgcggtcatg taaaagttga gcatcgacat ggccgagcga
     2341 aacggttcgg ctttgcagct ggctgcgctc ggccgagcgt tttagcgacc gggcgattgc
     2401 ccgcggatcg ttcagggaaa atactcccct cttcagatcg agagcatcgc tcgtatcggt
     2461 cacatgttgc gaccaacgtc tagtcttttt ccctggagat cgcgaccgtt
//
LOCUS       DASULZ010000232         5678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_941634, whole genome shotgun sequence.
ACCESSION   DASULZ010000232 DASULZ010000000
VERSION     DASULZ010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5678
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941634"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VFY96_04705"
     CDS             complement(<1..304)
                     /locus_tag="VFY96_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX5605791.1"
                     /translation="MALTKPIKGIIGACLTPFDDNDRVNYKALEKEVDFLVTDCDAVT
                     IAAVEAAEYTMLSRDERKELLKLATEMVGKRVPVILGCSSPSPREVIDLAEYSAKVG"
     gene            complement(308..964)
                     /locus_tag="VFY96_04710"
     CDS             complement(308..964)
                     /locus_tag="VFY96_04710"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX5605792.1"
                     /translation="MEMIDLSRLIYDGMPKIPILPDVHVQKFLSLEKGHPLNVTELSL
                     PCHAGTHVDAPVHIVPNGKSIDELPLECFAGNGAVISVKKNGGEEVTAKDLESSGVEV
                     KRGDILMLHTGWDEKFDSPDYNLHPYLSVDAAEWMVKRGIKMFGIDCITVDLPTPLRP
                     KGFDFPVHRTLLGNNVLIAENVTNLGSIVGKQTRILALPLRVKGSDAGHARIVAEVLH
                     "
     gene            complement(980..1720)
                     /locus_tag="VFY96_04715"
     CDS             complement(980..1720)
                     /locus_tag="VFY96_04715"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX5605793.1"
                     /translation="MAISSKEFVSEIQRMVRSKHSKDHPIIGMVEGGKLNRDQLKGFV
                     GQFYLFFPKPFPKPIAAMLSRCPDDPELEKMWIENVVEEGTGEITGTDSHKGLFIRFA
                     KACGYTKEELDAVEPLPETAAFLDWRELLMYQRSWLELYACQGFCLEGTANERMTRVV
                     NGLTRHYGFDRESEDIRYWTLHMGVDEEHMKVGPLAVERYAVSEPQQAMVRASVQKTL
                     DQFWLAFDGIKRAYVDNDPLYSRWLDGK"
     gene            complement(1886..2389)
                     /locus_tag="VFY96_04720"
     CDS             complement(1886..2389)
                     /locus_tag="VFY96_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605794.1"
                     /translation="MAAITLSHSQALRVIGQNLLSINCDSFDLITAGDDYVVTPHASE
                     AKVTVPSKKAFLVQLLGNRFRRGKADRRASTRLFFSRSEISIANLEGQAKRRIGSPTD
                     VRDLSFVLRVLGDYLDKKSASEFSILWSPHLIMVQHNEKEELFTRENVYDFGIRMYLK
                     RSNRPSG"
     gene            complement(2504..3007)
                     /locus_tag="VFY96_04725"
     CDS             complement(2504..3007)
                     /locus_tag="VFY96_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605795.1"
                     /translation="MTDSTHYVEELRAIGQMLAGRNIVDFELKHMDSGYFIQDLHEHT
                     ASLISRIQNWLHGAPYSTRAECLTISPAELERLSAEGRARRDKAGQLTQFRELSNILR
                     TVGAYLDSKEVELLELQKRPISITLQYRDKTGRHETEDRPVTSFYKFFLELYDKRGQT
                     EEPRRGN"
     gene            3531..4016
                     /locus_tag="VFY96_04730"
     CDS             3531..4016
                     /locus_tag="VFY96_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605796.1"
                     /translation="MATMVPDIVFLRKSCPQACLAFFQVSEGSVVRLQNPRVCLTKNP
                     IRATEYKRKDTETKPETPKPNRMPAQLRFRLSNAVLESAILAFELSDPLISSAEFFIE
                     RFGLCVCTERQLKFLAYLEIALQNSRILFFDFLFRDFQGRRNNVEVVAGSYRPRLALS
                     G"
     gene            4489..4734
                     /locus_tag="VFY96_04735"
     CDS             4489..4734
                     /locus_tag="VFY96_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605797.1"
                     /translation="MTQQDTIKNRERFQVALQNAASKQAIPLERLSWRDIPNRDASDL
                     IVEAGGKKRVFTITDNDMINDIEGQIDQILNVAIDVD"
     gene            4880..5155
                     /locus_tag="VFY96_04740"
     CDS             4880..5155
                     /locus_tag="VFY96_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605798.1"
                     /translation="MVENHIGGNESKEIFYAELEQFAREKIRGHLQDLLEQEVSEWLG
                     REKSERKINTVEQPGYRNGYGRVRRLTMSLGTIEIQRAPGARFERAI"
     gene            5488..>5678
                     /locus_tag="VFY96_04745"
     CDS             5488..>5678
                     /locus_tag="VFY96_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605799.1"
                     /translation="MKNCVRLFTRRRWGARVWHRDGGRKHREDKTRDRWVREVVADKG
                     YHSNELLKDLRVLGIRTYIS"
BASE COUNT         1379 a         1422 c         1525 g         1352 t
ORIGIN      
        1 caccgacttt tgcggagtat tccgctaaat cgatgacctc gcgaggggat gggctcgaac
       61 agccgagaat caccggtact cgcttgccca ccatctcggt ggcgagcttt aaaagctcct
      121 tgcgttcgtc acgcgacaac atcgtgtact cggcggcttc aaccgctgcg atcgtcactg
      181 cgtcgcaatc ggtgacgaga aaatcgactt ccttttcgag cgccttgtaa ttcacccgat
      241 cgttgtcatc gaacggggtc aggcaggcgc cgatgattcc tttgattggc ttggtgagcg
      301 ccataagtta atgcagaact tcagcgacaa tgcgcgcgtg accagcgtcg ctgcctttga
      361 cgcgtaatgg cagcgcgaga atgcgcgttt gcttgccgac gatgcttccc aggttcgtga
      421 cattctccgc gattaagaca ttgttcccga gaagcgtccg atgtaccggg aaatcaaacc
      481 ccttgggccg cagcggagtc ggcaaatcga cggtgatgca gtcgatgccg aacattttga
      541 tgcctctctt taccatccat tccgcggcgt ccaccgaaag atagggatga agattgtagt
      601 ccggactgtc gaatttttcg tcccatcccg tgtgtagcat gagaatgtcg ccacgcttca
      661 cctcgacgcc ggaggattcc aggtctttgg ctgtgacctc ttcgccgccg ttctttttta
      721 cgctgattac cgcgccattt ccggcaaaac attcgagcgg tagctcgtca atggatttgc
      781 cgttcggaac gatgtgaacc ggcgcatcca cgtgtgtgcc cgcatgacat ggcagcgaca
      841 actcggttac gtttaaagga tgtccttttt ccaggctcaa aaatttttgc acgtgcacgt
      901 ccggtaagat cggaattttc ggcatgccgt cataaatcag ccgtgagaga tcgatcattt
      961 ccatcgtcaa gctcctaatc tatttgccgt cgagccagcg cgagtacaga gggtcgttgt
     1021 cgacgtaggc acgcttgatg ccatcgaaag caagccagaa ttggtcgaga gttttctgca
     1081 ccgacgcacg caccattgcc tgttgcggct cactgactgc atagcgttca accgcaagcg
     1141 gcccgacctt catgtgctct tcgtccacgc ccatgtggag tgtccaatag cggatgtctt
     1201 cggattcgcg atcgaagccg tagtgccggg tcaagccgtt gaccacccgc gtcatgcgct
     1261 cgttggcggt tccttcgaga caaaaacctt ggcaggcgta aagctcgagc cagctgcgct
     1321 gatacatgag aagctcgcgc caatcgagaa atgcggcggt ctccggtagc ggttcgacag
     1381 catcgagttc ctctttggtg tagccgcacg ctttggcaaa acggatgaac aagcccttat
     1441 ggctatcggt gccggtaatt tctcccgtgc cttcttcgac gacattctcg atccacatct
     1501 tttcgagttc cgggtcgtcc gggcagcgac tcaacatcgc agcgatcggc tttggaaaag
     1561 gcttaggaaa aaataaatag aattgcccta cgaatccttt gagctgatct cggttgagtt
     1621 ttccaccttc gaccatgccg atgatcggat gatctttgga gtgtttactc cgcaccatcc
     1681 gctggatttc actaacgaat tctttgctcg atatcgccat ctcaccatcc gtcctttcgc
     1741 cttcgctgtc gtggaaaaat gatgccggtg catccttcgg accaaatttc tattatcagt
     1801 tcactctcgt tgagtccagt tcgttgagcg cgagctcggc attgctagtg tcaggatcga
     1861 ataggcgcga gactctctag gaaagttagc ccgaggggcg gttggatctt tttagataca
     1921 tgcggatccc aaaatcgtaa acattttccc gtgtgaagag ctcctctttt tcattgtgct
     1981 gaaccataat caaatgaggc gaccacaaga tggaaaactc gctggccgat ttcttatcca
     2041 gataatcgcc gagaacacgc aggacaaagg acaagtcgcg aacatccgtc ggactgccga
     2101 ttcgccgttt tgcctgacct tcgagatttg cgatcgagat ttcagaacgg ctgaagaaaa
     2161 gtcgagttga ggctctccgg tcggccttgc cccgccgaaa gcggtttccg agcaattgaa
     2221 ccaaaaacgc cttcttggac ggaacggtta cctttgcctc ggaggcatgc ggcgtgacga
     2281 cataatcatc accggctgtg atcaggtcaa aggagtcaca attgatggaa agcaggttct
     2341 gaccgatgac cctgagcgct tgtgaatgag agagagtgat ggctgccatc gtcgcatatc
     2401 tgggccgtac atcggtgtaa attcgaatcg agcaaactca tttttggcgc gaagaatggg
     2461 cttgtcaaca tgacagccgt gcggtggtaa agcattgctg gagctaattt ccgcgccgtg
     2521 gctcttctgt ttggccgcgc ttgtcataga gctcgagaaa gaacttgtag aatgacgtca
     2581 ctggacggtc ctctgtttca tgacgtcctg ttttgtctcg gtactgcagg gtgatagaga
     2641 ttggcctttt ttgaagttcc agcagttcca cttctttgga gtccagatag gcgccaaccg
     2701 tacgaagaat attggaaagc tcacgaaatt gggtgagctg cccggctttg tcacgacgtg
     2761 cccggccctc ggctgaaagt ctttcaagct ctgctggaga tattgtcaag cattcggcgc
     2821 gggtgctgta aggcgctcca tgaagccaat tttgaatgcg cgagatcaac gaagccgtgt
     2881 gttcatgaag gtcctgaatg aagtagccgg aatccatatg cttcaattca aaatcaacga
     2941 tatttctgcc cgcaagcatc tggccgatgg ctctgagctc ctcaacataa tgcgtcgaat
     3001 ccgtcatggc gcgtcctcct tccgccgtat tttaattact ttaggcggcc ggacaaacaa
     3061 aaatattgta gtaagagagc cggaccgacg aatagacggg agcagaatac tgccttaaac
     3121 actactgcac atcctgcata gcgtcgcgca gcgcatagcc aaaggacgtc gtcgaattac
     3181 tttgataaaa acagattctg tatatcgtca cgtttattcc aggctggaga ccgtaattct
     3241 attggcgccg ctttcggcac gcgcctgcgg agcccatcat cgctttggac gaaatgacgt
     3301 acagcgacag aatcttggct agaatcttgg ctatgaagtg ccgggagtct caccgatttg
     3361 tcgggtttat tagatcaaca atgtgaaact cagcacaaac atactccgag aagatttacc
     3421 gtaccgagta aagaacggaa gcgcagtcga gacttctaca accgggcaag gttttcggga
     3481 actccttcga gctatcgctg tgcatgcagc cgattgaacg agggatgcca ttggccacga
     3541 tggttcccga tatcgtcttc ctgcgcaaat cctgtccgca agcgtgtttg gctttctttc
     3601 aagtctctga gggatctgtc gtgcggctcc aaaacccgcg ggtgtgtttg acgaaaaatc
     3661 caatacgcgc caccgaatat aaacgcaagg acaccgaaac taaaccagaa accccaaagc
     3721 caaataggat gccagcgcaa ttgcgattcc gccttagcaa cgctgttctc gagagcgcta
     3781 ttcttgcttt cgagctctcg gatccgctga tctcttctgc cgagttcttc attgagcgat
     3841 ttggtctctg cgtatgcacg gagcggcagc ttaagtttct ggcctatttg gagattgctc
     3901 tccaaaattc caggattttg ttttttgact tcctgtttcg ggatttccaa ggtcgccgca
     3961 ataatgtcga ggttgtcgcc ggctcgtacc gtccacgttt ggctctcagc ggctaacgaa
     4021 atgcgcgaac tgagaacaca gcagatcaag gcagccaaca aataaatctt ttgcacggta
     4081 gcctccttgc tcacagcgag acatgggggt gtgagcaacg cacaataaaa agatagcagg
     4141 cgcctatagc gtgtcgctac ggggtgaagg tagtactgaa tatatgttca gctgcggccc
     4201 gtaatggccg catgcccagg agcacacaag ccagctggcg tggcgacggt ttagccggat
     4261 ccggtgtacc gagtagatgt tccaaacctg acggggacac ggctttttaa atgatccaat
     4321 aaccccatct cgaaagactc attcaaaatg gataatcttt gcttaagaac gggaggaaaa
     4381 aacagatgcg tcgcatgctc gctcggaaag acaacgcctg ttaatgtttc aacacgtcgg
     4441 ataaccttcg ggaactcgta gatctctatt tgaaggaggc tcggccaaat gacgcagcaa
     4501 gacacaataa aaaaccggga gcggtttcaa gtcgcattgc agaacgctgc atcaaagcag
     4561 gcgatcccgc tcgaacgtct cagttggcgc gatattccaa atcgtgatgc atccgatctg
     4621 attgtcgaag ctggcggcaa gaagagagtt tttacgatca ccgataacga tatgatcaac
     4681 gatatcgaag gacagatcga tcagattctt aacgttgcaa ttgacgtaga ttaggcagaa
     4741 cacttatgag atgagagaaa aggagctact gcgatgagct agaagcaaaa agaggaaata
     4801 gaaattaggt ggcgtaccct ttcgtagacg ttgaaacgaa aaggctgcct gttggggcag
     4861 gcagagaaag gatacgccgg tggtagagaa tcacattggg ggaaacgaat caaaggaaat
     4921 cttctatgcg gagttggagc agtttgcacg cgagaagatc cggggtcact tgcaggattt
     4981 gttggagcaa gaggtgagcg aatggctggg gcgggagaaa agcgaacgca agattaatac
     5041 ggtcgagcag ccgggttatc gcaacggcta cggcagggtg cggcggttga cgatgagttt
     5101 agggaccatc gagattcagc gggccccggg tgcgagattt gagcgagcga tttaagtcca
     5161 agatgctgcc gatgttcaag cggcagaccc cagaagtccg agagctgatc ccggagctat
     5221 atttacacgg actgagcagt ggcgatttcg agttagcgct acgtcatttg ctagcagccc
     5281 gtggtaaaag tcgctctact actatttttt atcgactaac ttgcccatct tcgcgtagag
     5341 tacgtgccat ggccatgggc aaacggaaac gaaaacttcg ccagcagtcg atgtggatcg
     5401 caacgcaaga tttgccgcga agcgcgtcgc aaccttttta cacgcaattg aatcggattt
     5461 tggagcagaa cggattcgac gactacgttg aagaactgtg ttcggctttt tacgcgccga
     5521 cgatggggcg cccgagtctg gcaccgggac ggcggccgaa aacatcgaga agataaaacc
     5581 cgagaccgat gggttcggga ggtggttgcg gacaaggggt atcacagtaa cgagctgctt
     5641 aaggatctga gggtgctcgg aattcgcact tatatttc
//
LOCUS       DASULZ010000233         4099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1042207, whole genome shotgun sequence.
ACCESSION   DASULZ010000233 DASULZ010000000
VERSION     DASULZ010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042207"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VFY96_04750"
     CDS             <1..304
                     /locus_tag="VFY96_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5605800.1"
                     /translation="IGGPILIAQMAGQQAQEGLGSFLAFLAVLSINLGVLNLLPVPVL
                     DGGHLLFFLVEAVTRKPVALKYREMAQQVGICLLALLMIYAFYNDIVRFFEKQVGG"
     gene            301..1050
                     /gene="tsaB"
                     /locus_tag="VFY96_04755"
     CDS             301..1050
                     /gene="tsaB"
                     /locus_tag="VFY96_04755"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX5605801.1"
                     /translation="MRILGIDSATGMASAALIEDEASIDEQRSDERHLVNADALDSIG
                     NCAEIIIPLINSLLEKKRLTPADLSAIAISIGPGSFTGLRVGLATAKGIAYSGGLPLI
                     GISTLLALAAGVKDFDGIICSLLDARKNDVYVGFFRRAGKILSRLSDDALLPIDRAIE
                     CARGYQEQTGAALVRVIGDAGEAHKQKLIGALGEKVVLGSEHKYPSIALQVALLARER
                     LLSESLDDLGSLVPIYLRRPEAEVRKLRVNY"
     gene            1146..1382
                     /locus_tag="VFY96_04760"
     CDS             1146..1382
                     /locus_tag="VFY96_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEX5605802.1"
                     /translation="MELREEHVIVSLLDKDPELKKFYDEHRELEKKLAEYQHKHHLTP
                     EEEVEMKKIQKLKLVGKDRMMQILEKHRHAGADH"
     gene            1398..3176
                     /gene="ilvB"
                     /locus_tag="VFY96_04765"
     CDS             1398..3176
                     /gene="ilvB"
                     /locus_tag="VFY96_04765"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEX5605803.1"
                     /translation="MKLTGAEIFCESLRREGVKTVFGLPGGVVLKIFDVLCQQKDLDL
                     VLTRHEQGAAHMAEGYAKATGRAGVVLVTSGPGMTNIVTGLADAYMDSVPLVAFTGQV
                     STDLIGNDAFQEADNVGISRPCTKHNVLVKDVNELAQTIKEAFYIATSGRPGPVLVDI
                     PKDVSTSKAEFKYPEKVNLRGYNPTVGGNKNQIRQAAEEILKAKKPILYVGGGAVFSD
                     AAQEIFELAEITQIPVTMTLMGLGSFPGNHPLSLGMLGMHGGYWTNMAMHQADLLIAV
                     GARFDDRVTGKLSEFCPEARVIHIDIDPTSIKKTFHAHIPIVGDVKAVLRQLNVILRS
                     LDGNSAQIAAQRKPWVQQIEDWKRAHPLVYKQDDKIIKPQFVIEKLYNLTKHEAIVAT
                     DVGQHQMFAAQYFKGSRPRTWLTSGGLGTMGFGFPAAIGAQIAFPDRQVLCITSEGSF
                     QMNLQELVVAAERKLPVKIVLLNNGVHGMVRQWQDLFYEGRYSASVLGTLPDFVKLAD
                     AYGIPGLRAVKPAEVESVLKDGLKQKGPVLMDIHTDPYENCYPMIPAGGAQHEMMLED
                     PPELKVAKKGGAHKRKVDEGEGVLPA"
     gene            3189..3710
                     /gene="ilvN"
                     /locus_tag="VFY96_04770"
     CDS             3189..3710
                     /gene="ilvN"
                     /locus_tag="VFY96_04770"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX5605804.1"
                     /translation="MEHIISVLVENKFGVLARVAGLFSARGYNIESLSVAPTLDPTTS
                     MITIVTVGDDRIIEQIMKQLNKVVEVLKVIDLTETHFLDRETALIKVHTKPEHRDEAI
                     RLAEIFRAKIVDSSANTYTIEITGDVDKVEAMINMLKPLGIKDLVRTGRIAISRETTR
                     TASPRREPALLKS"
     gene            3939..>4099
                     /locus_tag="VFY96_04775"
     CDS             3939..>4099
                     /locus_tag="VFY96_04775"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399623.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX5605805.1"
                     /translation="MQVYYDRDADLSVLRGKKIAVLGYGSQGHAHANNLRDSGVEVVV
                     GLRRGSSSWV"
BASE COUNT         1011 a          986 c         1131 g          971 t
ORIGIN      
        1 tataggcggt ccaattttga ttgcgcaaat ggccgggcag caagcgcagg agggcctcgg
       61 cagttttctt gcgtttctcg ccgtcctcag catcaatctt ggcgtgctga atttattgcc
      121 tgtgcctgtg ctcgacggcg gacatctatt gttctttctc gtggaagcag tgacgcggaa
      181 gccagttgca ctgaagtatc gcgaaatggc tcaacaggtt ggcatttgtc tgcttgcact
      241 actcatgatc tacgcgttct acaacgatat cgtgagattc ttcgagaagc aggtcggcgg
      301 atgagaattc tcggcattga cagtgccaca ggtatggcta gcgcagcttt aatcgaagat
      361 gaggcgtcaa tcgacgagca gagatcggat gaacgacacc tggtcaatgc cgacgccttg
      421 gattccattg ggaattgtgc cgaaattatt attccgttaa tcaactcgct tcttgaaaaa
      481 aaacgtttaa caccggctga tctctccgcc atagcaattt cgatcgggcc cggctcgttc
      541 actgggctac gggtcggcct ggcaacggcc aaaggtattg cctattccgg tggacttccg
      601 cttatcggta tctcgacctt gctggcgctt gcggcaggcg taaaagattt tgatggcata
      661 atttgttctt tgcttgatgc tcggaaaaat gatgtctatg tgggtttctt tcggcgcgct
      721 ggcaagattc tttcgcggtt gagtgatgat gctctgctcc cgatcgatag agcgatagaa
      781 tgtgcgcggg gttatcagga gcaaaccggc gctgctttag tgcgggtcat cggcgatgct
      841 ggcgaggcgc acaagcaaaa gttgatcggc gcgttggggg aaaaagtagt tttaggctcc
      901 gagcataagt atccatcgat cgctctgcag gtcgcgctac tcgctcgcga acgtctgctg
      961 agcgagtcgc tcgatgatct aggctcattg gttccaattt atcttcgtcg gcctgaggcg
     1021 gaagtgagaa aattacgtgt aaactattga aaaactaaga ggaaagttcg ttgacaaaga
     1081 tattagccta agttaaattg agtcccgcta aaaaattttt tcatactcga aaaggaggcg
     1141 cccgtatgga acttagagag gaacacgtga ttgtctccct gctcgataaa gatccagagc
     1201 taaaaaagtt ctacgacgaa catcgggaac tcgaaaagaa actggctgaa tatcaacata
     1261 agcatcacct gacaccggaa gaagaagtgg aaatgaaaaa aatccagaaa ctgaaactcg
     1321 tcggcaagga ccgcatgatg caaattcttg agaagcaccg tcatgccggc gccgatcatt
     1381 agaggagttg ttcatcgatg aaattgaccg gagcggaaat attctgcgag tcgcttagac
     1441 gtgaaggagt caaaacggtc tttggtctac caggcggagt cgtgctaaaa attttcgacg
     1501 ttctctgcca acaaaaggat ctcgatctcg tattgacccg tcacgaacag ggtgcggctc
     1561 atatggcgga aggctatgcc aaggctaccg gcagagccgg cgtcgtcttg gtgacgtcgg
     1621 gtcccggcat gacgaacatt gttaccggac tcgccgacgc ctatatggac tccgttccac
     1681 tggttgcttt tacaggtcaa gtttcaaccg acctgatcgg taatgatgcg tttcaagagg
     1741 cagacaatgt cggcatcagc cggccgtgca ccaaacataa tgtgctcgtg aaagacgtta
     1801 atgaattggc gcagacgatc aaggaagctt tctacatcgc gacaagcggc aggccgggac
     1861 cggtcttggt ggatattccg aaagacgtca gcacgtcgaa ggcggaattt aaatacccgg
     1921 aaaaagttaa cctgcgcggc tacaatccga cggtcggagg taacaagaac cagatcaggc
     1981 aagctgccga agaaatattg aaagcgaaga aaccgatcct ctacgtgggc ggcggcgcgg
     2041 ttttttccga tgccgcgcaa gagattttcg aactcgcgga aattacccag atcccggtga
     2101 cgatgacatt aatgggcctg gggagtttcc ccggcaacca tccattgtcg ctcggcatgc
     2161 ttgggatgca cggtggctac tggacgaaca tggcgatgca tcaggccgat ctcctcatcg
     2221 cggtcggtgc gcgctttgac gatcgcgtga ctggcaagct ttctgagttt tgtccggaag
     2281 cgcgcgtcat tcatattgat atcgatccaa cttccatcaa gaagaccttt catgcccaca
     2341 ttcccatcgt cggcgacgtc aaggccgttt tgcgtcagtt gaacgtgatt ctgcgatcgc
     2401 ttgacggtaa ctctgcgcag attgccgcgc aacgaaagcc atgggttcag cagatcgagg
     2461 actggaagcg agcccatcct ctcgtttaca agcaggacga caagatcatc aaaccgcagt
     2521 tcgtcattga aaagctctac aacctgacga aacacgaagc gatcgtcgcc accgatgtcg
     2581 gtcaacacca gatgtttgcg gcgcaatatt tcaagggcag ccggccgcgg acttggttga
     2641 cctcgggtgg acttggcacg atgggcttcg ggtttccggc agccattggt gcgcagatcg
     2701 cgtttcccga tcggcaagtt ctctgtatca ccagcgaggg tagttttcag atgaacttgc
     2761 aggagttagt ggtcgcggct gagcgtaaat tgcctgtgaa aattgttttg ctcaacaacg
     2821 gggtgcacgg catggtgcgg cagtggcagg atctgtttta tgaaggtcgc tattccgcga
     2881 gtgtcctcgg cacgttaccg gatttcgtga agctggccga tgcctacggc attcctggat
     2941 tgcgcgcggt caagcccgct gaagtggaat cggttttaaa agacgggctt aagcaaaaag
     3001 gtccggtgct gatggatatt cataccgatc cttatgaaaa ctgctatccc atgatccccg
     3061 ccggcggggc gcagcacgag atgatgctcg aggatccgcc cgagctcaag gttgcgaaaa
     3121 aaggtggagc acacaagaga aaggttgatg agggggaggg cgtacttcct gcctaatgat
     3181 tttcgcgaat ggaacatatt atatcggttc ttgtggagaa caaattcggc gtgctcgccc
     3241 gagttgcggg gctatttagc gcccgcgggt acaacatcga aagcttatcc gtcgctccta
     3301 cgttagaccc gaccacctcg atgatcacga tcgtcaccgt cggtgatgat cggatcatcg
     3361 agcagattat gaaacagctc aataaagtgg ttgaagtgct gaaggtaatc gatctgacgg
     3421 aaacccattt tctcgatcgg gaaaccgcgc tgatcaaagt tcatacaaaa cccgagcatc
     3481 gtgacgaagc gattcggtta gcggagattt tccgcgccaa gatcgttgat tcgtcagcca
     3541 acacgtacac catcgaaatt actggtgacg tcgacaaagt cgaagctatg atcaatatgc
     3601 tcaagcccct tggaatcaaa gacttagtcc gtaccggtcg tattgcaatc tctcgcgaaa
     3661 ctacacggac cgcgtcacca cggcgtgagc ccgctttgtt aaaaagctaa aatttcgttc
     3721 acgcagcttt ttccgctaaa cggcgtttct ttagagacgc aaaactgcgc ggtgcaccaa
     3781 aattgctgat tcattactgt agttgctttg ctagactgaa gagctgcaaa gcttcatgta
     3841 gcgctagcgg ttacggtttg taagtacttg cgagcgttgg tttgacttgc cagtgtccgc
     3901 tcggcatatt ggaagctcaa ccataggaga actagcgaat gcaagtctat tacgatcgag
     3961 acgctgatct cagcgttctc agaggaaaaa aaatcgcggt gcttggatac ggcagccagg
     4021 gtcatgcgca tgcgaacaat ctccgcgact ctggcgttga agttgtagtc ggtctcagac
     4081 ggggcagtag ctcgtgggt
//
LOCUS       DASULZ010000234         4104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_738331, whole genome shotgun sequence.
ACCESSION   DASULZ010000234 DASULZ010000000
VERSION     DASULZ010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738331"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2092)
                     /locus_tag="VFY96_04780"
     CDS             complement(<1..2092)
                     /locus_tag="VFY96_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605806.1"
                     /translation="MRSKLEARLERRTKAKKTKSPVGGKALQRLFFYLGERDPKLNDE
                     VIATIAPPRAARPKFGLTRTALRARPVSAAKAGRARRGTPAAKALALSITKAAAALTS
                     PRRRRRSTRRRVRAAAPAVAPAAWRAIGPSLIPKGQTYGSNVVHVIGRVSSIAVDPNH
                     PKHLLLGAAGGGIWESQNTGATWTARTDQMPSLAIGAIAFDPTNPKKVYAGSGEGNFY
                     ANLGAGVYKSVNGGTTWTVAASAPFVGVGFYDLLVDPQTPAVLYAATTNGFYKSTNSG
                     ASWSLKRAGKCWDISVHPTGGTVEILAAFADGLFVSTNAGNNFAAVALPSWPAGNLAR
                     LAVDHVKSAPAVAYAFAAIASAAHLWRRVGAAWTKIAAPTGLNINQAWYDWYVAAAPN
                     NQDQVYLGAIDAYRGTVSDSTASWTNITTQGSNSIHPDQHCLAFAPNNPQVIYAGNDG
                     GIYRSANSGATWKALNKGLGITEIEYLASDPSTWKWLMAGTQDNGTIRYTGSSALVWD
                     HIAGGDGGDCGINQLNSLNVYHSYYSVSLEHSTNKGNTWNELGLPWPPTLASLFYPPV
                     EVSGLTVAIGASSLLVTRNGVQPWTTVPLGLSSNEFPSAMRAVDANTLLIGTSAGRVL
                     RVWWTGAWNKAQLTSPAPRYISCIAVDPTNPQRFWVTISQIDGALVYRSDNAGSSWVS
                     CTAGLPAIPMNSVVV"
     gene            complement(2082..2273)
                     /locus_tag="VFY96_04785"
     CDS             complement(2082..2273)
                     /locus_tag="VFY96_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605807.1"
                     /translation="MTKRPGDKTPEPPGGRAAERLRMFEEARRGKKIFRVPKKKKKRA
                     ARNAKKDAIFKKGALSDEE"
     gene            2883..>4104
                     /locus_tag="VFY96_04790"
     CDS             2883..>4104
                     /locus_tag="VFY96_04790"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX5605808.1"
                     /translation="MEKRKRRALTWAGSIFGFFAIAAVGLTVFVTTLDQNRAKKYLAA
                     GVSKATGRELNINGDLKLDLGWISRLSASQIQFQNAGWSKHPQMAEVGLFDVEIDLWQ
                     LVRHFRVVLPSVTISEPNVVLEKNTEGSANWEFRTASVVAEPAVPENRGSFPVIQKII
                     IKDGVLLFDNQETKSQIKLKVTEAEGAGFLETPVKLKANGTYQGLPFTLSLDGGSYQN
                     LTSSREPYPLKIALSAGKFKGRIEGNLTEPLAMKGEDVTLDVQGDDLANLYPLIRLVF
                     PSTPPYRLKGHLKHDGKVWSFANFSGRVGDSDLSGNIRVDTAPKRPFMKADLVSNLLD
                     FDDLAGFIGGKPGTAPGETASAEQKKQAAAEKENDRIFPDQRYDLERLRAMDADVRLR
                     AKKILAPKLPIDDLN"
BASE COUNT          907 a         1151 c         1122 g          924 t
ORIGIN      
        1 ccaccactac cgaattcatt gggatcgccg gcaagcccgc cgtgcaacta acccaagagc
       61 tgcctgcatt atccgaccga taaaccaaag ccccgtcgat ttgtgagatc gttacccaga
      121 atcgttgcgg gttggtcgga tcgactgcga tgcagctgat atagcgggga gcgggcgagg
      181 tgagctgagc cttattccag gctccggtcc accagactcg gaggacgcgg ccggcgctgg
      241 tgccgatcag taacgtgttg gcgtcgactg cgcgcatcgc ggacgggaac tcgttgcttg
      301 agaggccgag cgggacagtt gtccagggct gaacgccgtt gcgcgtgacc agtagcgaag
      361 aggccccaat cgcaactgtc aatcccgata cttcgaccgg cggatagaac agagaggcca
      421 aggtgggcgg ccatggaagg ccaagctcgt tccacgtgtt acccttgttg gtcgaatgct
      481 caagtgacac ggaataatag gagtgataca cgtttagcga attaagctga ttgatgccgc
      541 agtcacctcc gtcgccgccc gcaatatggt cccagaccag cgccgacgag ccggtatatc
      601 ggattgtgcc attgtcctgc gtgcctgcca tcaaccattt ccacgtactc ggatcgctcg
      661 cgaggtattc gatctctgtg atgccaagcc ccttgttgag cgctttccag gttgcgccgc
      721 tatttgccga acggtaaatc ccgccatcgt tgcccgcata aatcacctgc ggattatttg
      781 gcgcgaaggc cagacaatgt tggtccggat ggatgctgtt gctgccttgc gtggtaatgt
      841 tggtccagct tgcagtggag tcgctgaccg ttccccgata cgcatcgatg gcgcctaagt
      901 agacttggtc ttgattattg ggtgccgcgg ccacgtacca gtcgtaccag gcctgattga
      961 tattcagtcc cgtcggcgcg gcgattttcg tccaggctgc gcccacgcgg cgccacaaat
     1021 gcgccgccga agctatagcg gcaaatgcat acgctacggc cggcgcgctt ttcacatgat
     1081 cgacggcaag ccgcgccaaa ttaccggcgg gccaggacgg aagggcgacg gcagcaaagt
     1141 tgttcccggc gttggttgac acgaaaaggc catcggcaaa cgcggcgagg atctccaccg
     1201 tccctccggt cggatggacg ctgatgtccc agcattttcc cgcccgtttc aaactccacg
     1261 aggcaccgct attggtcgat ttgtagaatc cgttggtcgt cgcggcatag agcacggccg
     1321 gcgtctgagg atcgacaagt aggtcataaa aaccgacacc gacaaacggc gccgaggcgg
     1381 ctacggtcca agtcgtcccg ccgttcacgg atttgtacac gccggcgccg aggttcgcgt
     1441 agaagtttcc ttcgccgctt ccggcataga cttttttcgg gttcgtcgga tcgaacgcaa
     1501 tggctccgat cgcgagcgaa ggcatctgat cagtacgcgc cgtccaggtt gcccctgtgt
     1561 tttggctttc ccagatgccg ccgccggctg cgcctaacag caaatgcttc ggatggttcg
     1621 gatccacagc aattgacgac actcggccga tcacgtggac aacgttgctt ccgtacgttt
     1681 gccctttagg aatgagcgat ggtccaatgg cccgccaggc ggctggggct acagcgggag
     1741 ccgccgccct tactcggcgt ctcgtgctcc ttcgtctccg cctaggactg gtgagcgcag
     1801 cagcagcttt agtaatcgac agagccaacg ccttggcggc gggcgtgcct ctgcgcgcgc
     1861 gccctgcttt ggccgcgctt accggcctcg ctcgcaaagc agtccttgtc agtccgaact
     1921 taggccgtgc ggcgcgaggt ggggcaatgg tcgctatgac ttcatcgttg agcttgggat
     1981 cgcgttcgcc aagataaaaa aacaaccgct gcagcgcttt gccgccgacg ggcgatttgg
     2041 tttttttcgc cttggtgcgc cgttcaagtc tcgcctcaag cttactcctc atcggataag
     2101 gctcccttct tgaagatcgc gtccttcttc gcattccttg ccgctcgctt tttctttttc
     2161 tttggaaccc gaaaaatttt cttgccgcgc cgcgcttcct cgaacattcg cagtcgctcc
     2221 gctgcgcggc cacccggcgg ttccggtgtt ttatcgcctg gacgcttcgt catggtcgct
     2281 ccaatccttt ggggcttgat cggtagctgt cgaagtagat tgcgttccgg ttatcgaagc
     2341 cgaaacaaaa tgtcaaattt tttccgatac ggatcacgat cttatttacg aggaatctta
     2401 cgttcaccgc cgagcaccga gactttcctc gcgcatcata atcccaactg ctttccatgt
     2461 cggacagata gcaaatcttc acctcacggt gccggtcgct gtttatgcag tgccagacgc
     2521 aatccagaaa aagtgaatgt cagagagcgc cactgagctc aatgtcaaaa ataaagattc
     2581 gcttggaagc agaaaaaatc ataactgatg ctcgggaaat tcctccaagc agccattaca
     2641 aggccacgtt gacgacctga tcggactgaa atccgagaaa gctagaccgg cggctggcct
     2701 ttcaccgcga cgtcatccgc tgtcgctcga aaactccacg tttaatgcag ctaatcgcca
     2761 atcaagctga ttcgcgatga ctttgggcct taaaacctcc atttttgata aacgcccttc
     2821 gtccagtggt acatcttttg cggccgaaca cgtcgaaacc aagcagagag acgaggtgcg
     2881 caatggaaaa gcgaaaacgt cgcgctttga cttgggcggg ttctattttt ggtttctttg
     2941 ctattgctgc ggttggactc acagttttcg ttacgactct ggatcagaac cgcgccaaga
     3001 aatatttagc agcaggcgtc agcaaagcga ccggtcgaga attaaacatc aatggggacc
     3061 tcaaactaga tctcggctgg atctcaagac tcagcgccag ccagattcag tttcagaacg
     3121 ctggctggag caaacacccg cagatggccg aagtgggtct gttcgatgtc gagatagatc
     3181 tctggcaact cgttcgacat tttcgtgtag ttctccccag tgtgacgatt tctgaaccta
     3241 acgtagttct cgaaaaaaat actgagggct cagcaaattg ggagtttcgc accgcgtccg
     3301 tagtggccga gccggcagta cccgagaacc gcggtagttt tcctgtgatt caaaaaataa
     3361 ttatcaaaga cggcgtactc ctgtttgata atcaggaaac caagagccag ataaaactca
     3421 aggtgactga ggccgaaggt gcgggatttt tggagacacc ggtcaagctc aaggccaacg
     3481 gcacatatca aggactgcca ttcactttat ctttagacgg cggatcttat caaaacctaa
     3541 cgagttcaag agagccatat cctttaaaaa tcgcccttag cgccggaaag tttaaaggac
     3601 gaatcgaggg caatctcacc gagcccttgg cgatgaaggg ggaggacgtc acgctagacg
     3661 ttcaaggtga cgacttggcg aatctctacc cgctcattcg ccttgtgttt ccatcgacgc
     3721 cgccgtaccg cctcaaaggt catttaaagc acgacggaaa agtttggtca ttcgccaatt
     3781 tttctggtcg ggtgggcgat agcgacctct cgggtaacat ccgtgtggat actgcgccga
     3841 aacgtccctt catgaaggcc gatctcgttt ccaatttgct cgatttcgac gacttggcgg
     3901 gtttcatcgg aggaaagcct ggcacagctc caggcgagac agcttcagcg gagcaaaaaa
     3961 agcaagctgc cgctgaaaag gagaacgatc gaatatttcc agatcaacgc tacgatcttg
     4021 agcgtttgcg agccatggat gcggacgttc ggctgcgggc gaaaaaaatt ctcgcgccca
     4081 aactgccgat cgacgacctc aatg
//
LOCUS       DASULZ010000235         2684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_637468, whole genome shotgun sequence.
ACCESSION   DASULZ010000235 DASULZ010000000
VERSION     DASULZ010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637468"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VFY96_04795"
     CDS             complement(<1..340)
                     /locus_tag="VFY96_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605809.1"
                     /translation="MKLPRDNETSQGQGLRSSSAARDPFIHIRGARQNNLKDIDVKVP
                     LNALTVVTGVSGSGKSTLAFDILYAEGQRRYVESFSAYARQFLDRMDKPDVESIEGIP
                     PTIAIDQSRPV"
     gene            complement(337..606)
                     /locus_tag="VFY96_04800"
     CDS             complement(337..606)
                     /locus_tag="VFY96_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605810.1"
                     /translation="MSEVKSDRSVTAEKVALLAEERKLRRLGRAMDLAAALLWQVDLT
                     LEEARDVVNHAKQTALQLFPDKEATFDLIYGPRFRRILSEKYRLQ"
     gene            713..1399
                     /locus_tag="VFY96_04805"
     CDS             713..1399
                     /locus_tag="VFY96_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEX5605811.1"
                     /translation="MRISEIFFSIQGEGRLIGVPSVFIRTAGCNLRCVWCDTPYTSWQ
                     PVGAAWSIGTILAEVQKHPTEYVVVTGGEPLLAPKIEALCRELKESGRHITIETAGTI
                     FKPIRCDLISLSPKLANSTPWRRKGGKFALQHERQRLNLPVIQKFLDNYDYQLKFVVA
                     EKKDFDEIAEILDQLKTVDRSRVLIMAEGTTTARLRVRGRWIVELCKRYGYRFTPRLH
                     IELYGNQRGT"
     gene            complement(1547..>2684)
                     /locus_tag="VFY96_04810"
     CDS             complement(1547..>2684)
                     /locus_tag="VFY96_04810"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX5605812.1"
                     /translation="SRQALRRAYQNYANQTPQQIVAHLLAARKNPQRRATILFFAWHL
                     GKSESDLETWLQQNRIPTKGIDLVKLYRAYSNPRLGLTDYAFLLGAHPLDLWCATQFR
                     NDPNLSWEKLYASSGEARRMASAWLLSGRNRRAQDLRLRIRMEKDAFARMTPYWQKLG
                     FPFKTMVPTYATAIGSSSDRPVALAELVGILVNDGVRRPTQSLTNVHFASGTPYETLF
                     QRGTADGEQVLDPQIASIVRQTMAGVVEQGTARRLSGAFKLSDGKIVTVGGKTGSGDN
                     RFETFNRYGGVISSRATNRTATFVFYIGERYFGVITAYVQGREAENYHFTSALPVTLL
                     RLLAPTLLPKVDKDFKQPAVTIPASAPGAKPDNSTGLERPAVTN"
BASE COUNT          583 a          731 c          790 g          580 t
ORIGIN      
        1 gcaccggacg gctttgatca atggcaatgg ttggtggaat cccctcgatg ctctccacat
       61 cgggcttgtc catccggtcg agaaactggc gggcataggc ggaaaagctc tccacataac
      121 gacgttggcc ttcggcatac aagatgtcga aggccaaggt tgattttccc gagccgctga
      181 cgccggtcac aaccgtgagc gcgttcagcg ggactttcac gtcaatgtct ttgagattgt
      241 tttgccgggc accgcgaata tggataaatg gatcacgggc tgccgagcta gaccgcaatc
      301 cttggccctg tgaggtttcg ttgtcgcgag gaagcttcat tgcaaacgat atttttcgga
      361 caaaatacgg cgaaagcgcg gaccgtagat caaatcgaag gtcgcttctt tgtcaggaaa
      421 aagttgcaac gccgtctgct tggcgtgatt gacgacatcg cgcgcttctt ccagggtcaa
      481 atcgacctgc caaagcagcg ccgccgcgag gtccatggct cggccgagcc ggcgcagctt
      541 gcgctcttcg gcgagtaacg caactttttc ggcggtcaca ctccgatcgg attttacttc
      601 gctcatagaa ggaagatttt cgtcgtgcgc taaccttcta catttagcac aaggcggcag
      661 tgatgcaaga ttggatttga atgccgcacc ggcactttgc tagcttgagt gagtgcggat
      721 cagcgaaatc tttttttcca tccaaggcga gggccggctg atcggcgtgc cgtcggtatt
      781 tatccggacc gcgggctgca atcttcgctg cgtctggtgc gatacgccct atacttcgtg
      841 gcagccggtc ggcgccgcat ggagcattgg aaccatcctc gctgaagttc aaaagcatcc
      901 aaccgaatac gtcgtcgtga ccggtggcga gccgctcctc gcgccgaaaa tcgaagcact
      961 ttgccgggag ctcaaggaga gcggccgtca tatcacgatc gagacggccg gcacgatctt
     1021 caaaccgatc cgctgcgatt tgatttctct cagccccaag cttgcgaatt ccacgccctg
     1081 gcgacggaaa gggggaaagt ttgcgctgca gcatgagaga cagcgattga atttgccggt
     1141 cattcaaaaa tttttggata actatgatta ccagttaaag tttgtcgtgg cggaaaaaaa
     1201 agatttcgac gagatcgccg agattctcga ccagctgaaa accgttgacc ggtcgcgggt
     1261 cttgatcatg gccgagggca caaccaccgc gcgcctgcgc gtcagaggac gctggatcgt
     1321 cgagctgtgc aaacgctatg ggtaccgatt tacgccgcgc ttgcatatcg aactctatgg
     1381 caatcagcgc ggcacgtgat agctgaaagc gctcgatatg gccctgtggg cctactcggg
     1441 caatcggctt accagagggg ccccggcaag aatttatgcg aaggcaccgg ttaacacgaa
     1501 aacatgcgaa gtctctgtct ccgtgtcaaa tacctcaaat acccaatcaa ttcgtgacgg
     1561 ccggccgctc taagccagtg gaattgtctg gctttgcccc aggggctgaa gccggaatgg
     1621 tgacggcggg ctgcttgaaa tctttgtcca ctttcgggag gagcgttggc gcgagcagcc
     1681 gtaacagcgt cacaggaagg gcgctggtga aatggtagtt ttccgcctcg cgaccttgga
     1741 cgtacgcggt gatcacgccg aagtagcgct cgccaatata gaagacgaag gttgccgtgc
     1801 ggttcgtggc gcgcgaactg atcacgccac catagcgatt gaatgtttca aagcggttgt
     1861 cccccgagcc ggtctttccg ccgaccgtga caatcttgcc atcagataat ttgaaggccc
     1921 cgctcaaacg gcgtgcggtt ccctgttcaa cgactccggc catcgtttgc cgtacgatgg
     1981 acgcgatttg cggatcgagg acttgctccc cgtccgcggt accgcgctga aaaagcgttt
     2041 cgtagggcgt cccggaagcg aagtgcacgt tggtaaggct ctgagttgga cggcgcacgc
     2101 cgtcgttcac tagaattccc accaattctg ccaatgcgac cggtcggtct gaagagctgc
     2161 cgatggcagt agcataagtc ggcaccatgg ttttgaaggg aaaaccgagc ttctgccagt
     2221 agggcgtcat gcgggcaaaa gcatcttttt ccatgcgaat gcgcaaacgt aaatcctgcg
     2281 cgcggcgatt gcgaccgctg agcaaccagg ctgacgccat gcggcgcgct tcaccgctcg
     2341 acgcgtaaag cttttcccag gagagattcg gatcattacg aaactgggtc gcacaccata
     2401 gatcaagggg gtgcgcgccg agcagaaaag cgtagtcggt gagacccaag cgcggattgc
     2461 tgtaggcacg gtacaacttt accagatcga tgcctttggt cgggatgcgg ttctgctgca
     2521 gccaagtctc cagatcgctc tcgctcttgc ccagatgcca cgcaaaaaac agaatcgtcg
     2581 cgcgacgctg ggggtttttt cgggctgcga gaagatgcgc gacgatttgc tgcggcgtct
     2641 ggttggcgta gttctgatag gcgcggcgca gcgcctgacg cgat
//
LOCUS       DASULZ010000236         2666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_537015, whole genome shotgun sequence.
ACCESSION   DASULZ010000236 DASULZ010000000
VERSION     DASULZ010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537015"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..645)
                     /locus_tag="VFY96_04815"
     CDS             complement(175..645)
                     /locus_tag="VFY96_04815"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX5605813.1"
                     /translation="MTSSPVNELSFQDQGSVRYCHGCGADNEKGLRIKSYWDGDEAVA
                     TWQPQAHHCAGTPDRLNGGIIASLIECHSLNLAIARAYRDEQRAIGSTPRIGYVTANL
                     NVSYLKPTPLGEIIELRARIDKLEGRKIWVSCSLTALGELRATGTVLGVRVNWD"
     gene            complement(642..1037)
                     /gene="msrB"
                     /locus_tag="VFY96_04820"
     CDS             complement(642..1037)
                     /gene="msrB"
                     /locus_tag="VFY96_04820"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006101300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX5605814.1"
                     /translation="MEKIKKSEDEWKKELTEEQYYVCRQKGTERAFTGKYWDNHEKGV
                     YRCACCGNELFTSDTKFDSGTGWPSFWAPVAGESVQTEEDNSLMMRRTEVTCSKCGAH
                     LGHVFDDGPQPTHLRYCINSASLEFDKNK"
     gene            complement(1209..1700)
                     /locus_tag="VFY96_04825"
     CDS             complement(1209..1700)
                     /locus_tag="VFY96_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015852257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEX5605815.1"
                     /translation="MPSFDIVNRVDLQEVDNAVNITKKAILSRYDFRNSKTEITIDKK
                     EKKIRVVTEDDMKLRAVQDSLIENLVKRRVDRKCLELKTAEPAAHGMIQREIAIKEGV
                     DSDTARNIVKLIKDKKLKVQAAIQENQVRVTGKKIDDLQEVIQMLRTSPMPIPLQFIN
                     MQR"
     gene            complement(1719..2243)
                     /locus_tag="VFY96_04830"
     CDS             complement(1719..2243)
                     /locus_tag="VFY96_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5605816.1"
                     /translation="MAAKKIISKAIRVVDWMSEGVLAVETFDSIAVARRLMAKHRVNQ
                     LPVLDNDRLVGIVTDRDVRDAYPTSLMINRAEEIDRFADKITVEEVMTHDVFVVRPDT
                     ALATAVGLLRRHRIGALPVIKDQKLVGIITRSDILDFVLRNGLGQKRKRAKSPASGEK
                     PSRRKRRRASAKAS"
     gene            complement(2243..>2666)
                     /locus_tag="VFY96_04835"
     CDS             complement(2243..>2666)
                     /locus_tag="VFY96_04835"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEX5605817.1"
                     /translation="GVDQYLAGEAKRRGKPTSGLETLEFQIGLLDQLSKQNQEAMLRE
                     TVRELDLLDRNIDEIVRSWVEGDEAVLAKLLLAGMVEFPEIHQKVIVERNRRWAPEIE
                     KLLEQGSGALIVVGAAHLVGRDGVIEMLKTKGYSVEQK"
BASE COUNT          523 a          786 c          658 g          699 t
ORIGIN      
        1 tgcggatcat tgcgcatccc gagacggctc ggcccgccgc agccaagtcc cgccacactg
       61 actttcaatc cggtttttcc gagtgtggtg tattccatgt gttgtaaacg tgccaccaac
      121 ttttctgacg gttcgaacgg cgtgaacttg tttgatcggc ttgaacgact cttctcagtc
      181 ccagttcacc cgtaccccaa gcacggtgcc cgtcgcgcgc aactcaccga gtgcggtgag
      241 gctgcagctg acccaaattt ttcgcccctc gagcttgtcg atgcgcgcgc gcaactcgat
      301 aatctccccg agcggcgtcg gcttcaggta cgaaacgttt aaattggctg tgacatagcc
      361 gatccggggc gtgctgccga tcgcgcgctg ttcgtcgcga taggcgcgtg cgatggccag
      421 gttgagcgag tggcactcga tcagtgacgc gatgatgccg ccgttcaaac gatccggagt
      481 gccggcgcaa tgatgagctt gcggttgcca tgtcgccacc gcctcgtcac cgtcccagta
      541 gctcttgatg cgtagtccct tttcattatc tgcgccgcag ccgtggcagt accgaaccga
      601 gccttgatcc tgaaacgaaa gctcgttcac cggcgatgaa gtcatttgtt tttgtcgaat
      661 tccaaagaag cggaattgat acaatagcgt agatgagtcg gctgcggtcc atcatcaaac
      721 acatgtccga gatgcgcacc gcacttgctg cacgtcactt cggttcgccg catcatgagg
      781 ctattgtcct cctcagtttg gacactctct ccggccactg gggcccaaaa actcggccag
      841 ccggtgccgg aatcgaattt ggtatcggac gtaaaaagtt cattgccgca gcaggcgcat
      901 cgatacactc ccttctcatg attgtcccaa tatttgccgg taaaggctcg ctcggtgccc
      961 ttttgacggc agacgtaata ctgctcctcc gtcagctctt tcttccattc atcttcgctt
     1021 ttcttgatct tttccatctt acctccggtc gacaaggaga atttcgccaa cgtcatcgtg
     1081 cgggctagct gaaacaatca tgttttcgtc acctccaaca tatcctccgc tatcgcagaa
     1141 tttgtgagac ctcgtgcttg gactccgaaa gccgagttcc cgattaggcg gagcgcgtaa
     1201 cgaggacctc accgttgcat attgatgaat tgcagcggaa tcggcatcgg actggtgcgc
     1261 agcatttgga tcacttcctg cagatcatca attttctttc cggtgacgcg cacttgattt
     1321 tcctggatcg ccgcttggac tttgagtttc ttgtctttga tcagtttaac gatatttcgc
     1381 gccgtgtccg aatcgacgcc ttctttgatg gcgatctcgc gctgaatcat gccatgggcc
     1441 gccggctcgg cggtttttaa ctcgaggcat tttcgatcga ctcggcgttt gaccaggttc
     1501 tcgatcaaag aatcctgcac tgcccgcagc ttcatgtcat cctcggtgac gacgcgaatt
     1561 tttttctctt ttttatcgat cgtaatttca gttttcgaat tgcgaaaatc gtagcgcgaa
     1621 agtatcgcct tttttgtgat gttgacggcg ttatcgactt cctgaagatc gacgcgattg
     1681 acgatatcga aggatggcat tcctgcacca cgtgtgtatt agctcgcttt ggcgctggcg
     1741 cgccgccgct ttcgccggct gggtttttca cctgaggcag gtgacttggc gcgcttgcgc
     1801 ttctgcccaa ggccgtttcg caggacgaaa tcgagaatat cgctgcgggt aatgatgccc
     1861 acgagctttt gatctttgat caccggcaac gcaccgatgc gatggcggcg cagcagtccc
     1921 accgccgttg ccaatgcagt atccggcctg acgacgaaaa catcgtgcgt catcacttct
     1981 tccaccgtaa tcttgtcggc gaatcggtcg atttcctcgg cacggttgat catcaggctg
     2041 gttggataag cgtcacgaac gtcccgatcg gtcacaatac ccaccagacg atcgttatcg
     2101 agcaccggta attgattgac ccgatgcttg gccatgagtc gccgggccac agcaatgcta
     2161 tcgaatgttt ccaccgcaag caccccttcg ctcatccaat cgacgacgcg aatggctttg
     2221 gaaattattt ttttcgccgc cattactttt gctccacgct ataacctttt gttttcaaca
     2281 tctcaataac accgtctcgg cccaccagat gcgccgcgcc gaccacgatc aacgccccgc
     2341 ttccttgttc caacaatttt tcgatttccg gtgcccaccg gcgattgcgc tcgacaatca
     2401 ctttttgatg gatctcggga aactccacca tgccggccag aagtagcttt gccaagaccg
     2461 cctcgtcacc ctctacccaa gagcgcacga tttcgtctat attccggtcg agcaagtcca
     2521 actccctgac ggtttcgcgc agcatcgctt cttgattttg tttcgatagc tggtccagta
     2581 gaccgatctg aaattccaaa gtctccagtc cgctggtcgg ttttccccgt cgtttggctt
     2641 cacccgcgag atattgatcg acaccg
//
LOCUS       DASULZ010000237         3402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1449333, whole genome shotgun sequence.
ACCESSION   DASULZ010000237 DASULZ010000000
VERSION     DASULZ010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449333"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..469)
                     /locus_tag="VFY96_04840"
     CDS             complement(59..469)
                     /locus_tag="VFY96_04840"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX5605818.1"
                     /translation="MNDAGSNLDQDRSSSFFQTVKIFGVGLASALHTRLDLFATELEE
                     ERERLKQALILAVLVIFGLAFGFALINIFLVALFWEHGWIAAIGILALLYFGIACGAA
                     LKLRSAILRRSGLFPATLAELGKDCAQLRKSLRE"
     gene            complement(480..920)
                     /locus_tag="VFY96_04845"
     CDS             complement(480..920)
                     /locus_tag="VFY96_04845"
                     /inference="COORDINATES: protein motif:HMM:NF017742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HEX5605819.1"
                     /translation="MLNQSGTSVVCAGVTAAIHPSGLNTRAPAIIMHESKAKTREVDM
                     DGEGFNNKERLVNDFRALMTDAEELLRVTASQAGEKIGAARQRIEQSLTEGRKALADA
                     EQRVVTKSKECAEIADDYVRENPWSAVGMAAAVGLVFGLMIRRK"
     gene            973..1995
                     /locus_tag="VFY96_04850"
     CDS             973..1995
                     /locus_tag="VFY96_04850"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5605820.1"
                     /translation="MIHWARFYGICTTVMVLLMAPLIAMAQGSPDQKIIDAAKKEGEL
                     IYYTTMTLDQSKIVVDRFQKKYGIKTTLFRTGGGPLLNKIFTEARGGRYDWDVVVGRG
                     EMVLPLIERKLVASYRSPETKMIEEQLFDKEGYWTAYYVNSYVLGWNTQLVKKPDVPK
                     TYEELLNPKWKGGQISVDTEAYGMLEGLKRVWGKEKAVDYFKRLAALDPVLKRGNTER
                     VQLVVAGEYPLILAYNQTIQRMNSRGAPIDWLPLEPAVTQVNPVMLAAKAPHPNAARL
                     FYDFVLSKEGQEMLRGFQRIPVRKDVEPDPPRLFRGFKIAVEDPEDYKDFDASVKQYL
                     AIFKLR"
     gene            complement(2031..3323)
                     /locus_tag="VFY96_04855"
     CDS             complement(2031..3323)
                     /locus_tag="VFY96_04855"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX5605821.1"
                     /translation="MSNVAIIGAQWGDEGKGKVVDLFTRQADIVVRFQGGNNAGHTLV
                     VDGKKTVLHLVPSGALHSDKLCIIGNGVVVDPEVLIEEIQALKAQGHLTSADQLRISE
                     QAHVIMPYHKAIDLARERLRGKGKIGTTGRGIGPAYEDKVARVGIRFVDLLEEDTFRE
                     KLERNIQEKNFYLKAILKEKALDFEEIHDRYGEFREILKSYVTNTGVLLDEQIHAGKR
                     VLFEGAQGTLLDVDHGTYPYVTSSSTITGGACSGSGVGPQKIQQVIGISKAYTTRVGS
                     GPFPTELDGAEGETLRREGAEFGATTGRSRRCGWFDAVGVRHAVRMNGMTGIALTKLD
                     VLTGFKKLPICTAYRYQGKLVSEFPASCKVMERAEPVYEELDGWEEPLDKVRSFSELP
                     KTAQGYVKRIEQVIGTEIILVSVGPGREQTILLKNPFD"
BASE COUNT          744 a          934 c          949 g          775 t
ORIGIN      
        1 ctcgagtcga atcttgtcaa tcaaggcttg tttgcggcgg gcgatctctg cgaaacgttt
       61 actcacgaag tgactttctc aactgcgcac aatccttgcc cagctcggcg agggtggccg
      121 gaaacagccc ggaacggcgc aggatagcgc tacgcaattt aagcgcggcg ccgcaagcga
      181 tgccgaagta aagcagcgcc aggatgccga tcgcggcgat ccagccatgc tcccaaaaaa
      241 gggcgacgag aaagatgttg atcagagcaa aaccgaaggc gagcccgaaa atgaccagca
      301 cggccaagat cagagcttgt ttgagccgtt cccgttcttc ttcgagctcg gtagcaaata
      361 agtcaagacg ggtgtgcagc gccgaggcaa gacccacccc gaaaattttc accgtctgaa
      421 aaaaactcga cgagcgatcc tggtcaaggt tgctgcccgc gtcgttcatc gatggcagtt
      481 tatttgcggc gaatcataag cccaaacaca aggccgacgg ccgctgccat tccgactgcg
      541 ctccagggat tttcccggac ataatcatcg gcgatttcgg cgcattcttt cgattttgtt
      601 actactctct gttcggcatc agccaaggct tttctgcctt ccgtgaggct ttgctcgatt
      661 ctctggcgcg ccgcgccgat cttttcgccg gcctggctag ccgtcaccct gaggagctct
      721 tcagcgtcag tcatcagcgc cctgaagtcg ttaactagtc tttctttgtt attgaatcct
      781 tcgccgtcca tgtctacctc ccgtgtctta gctttggatt catgcatgat aatcgcgggt
      841 gctctggtat tcaagccgct gggatgaatc gctgccgtga cgccggcaca gacgacactc
      901 gtgccagatt gatttagcat ttctaaatgg tatccgtgct ttggtcccgc agcggccaag
      961 gaggtaatgg caatgatcca ttgggctcgg ttttatggaa tctgcaccac ggtgatggtt
     1021 ttgctgatgg cgcccctgat cgccatggca cagggcagcc cggaccagaa gattattgat
     1081 gcggcaaaaa aagaaggcga gttgatctac tacacgacaa tgaccctcga tcagagcaag
     1141 atagtcgtcg atcggtttca gaagaagtac ggcatcaaaa cgacgctgtt tcgcaccggt
     1201 ggtggaccct tgctgaacaa gattttcacc gaggcccgcg gcggccgcta tgactgggat
     1261 gtcgtcgttg ggcgtggcga aatggtgctg ccgttgatcg agcgcaagct agtggcatcc
     1321 taccgttcgc cggaaaccaa aatgatcgaa gagcagctct tcgacaagga gggatactgg
     1381 actgcgtatt acgtcaacag ctacgttctc ggatggaata cccagctggt gaagaagccc
     1441 gatgttccga aaacgtacga agagcttttg aatcccaaat ggaagggcgg gcaaatctcc
     1501 gtcgatacgg aagcttacgg aatgcttgag ggattgaaac gcgtctgggg caaagaaaag
     1561 gcagtggatt acttcaagcg tctcgccgca ctcgatcccg tcttgaagcg aggaaatacc
     1621 gagcgcgttc agctcgtcgt cgcgggcgaa tatcccctga tccttgcgta caatcagaca
     1681 attcagcgca tgaactcgcg cggcgcgccg attgattggc tgccgctcga gccggccgtg
     1741 acgcaggtca atcccgtgat gctcgctgcc aaggcgccac atccaaatgc tgcgcgcttg
     1801 ttctacgatt tcgttctgtc caaggaaggg caggagatgc tgcgcggttt tcagcgtata
     1861 ccggtgcgca aagatgtcga acccgatccg ccgcgactgt ttcgcgggtt taaaatcgcc
     1921 gttgaagatc cggaagacta caaggacttc gacgcatcgg tgaagcagta cctggcgatt
     1981 ttcaaattaa gataagcgta ggagcgcagc atgctgcgcc cgttggcgct tcaatcgaac
     2041 gggtttttca gcaggatcgt ttgctcgcgg cccggtccga cggagacgag aatgatctcg
     2101 gtaccgatca cttgttcaat gcgcttaacg tagccttgcg cggttttcgg caactcggaa
     2161 aaacttcgaa ccttgtccag cggctcctcc catccgtcca gttcttcata gacgggctct
     2221 gcgcgttcca taactttgca gctcgcgggg aactcgctga cgagttttcc ctgatagcgg
     2281 tacgcggtac agatgggaag ttttttgaag ccggtaagga cgtcgagctt agtcaacgca
     2341 atgccggtca tgccgttcat tcgtacagcg tgacgcacgc cgacggcatc gaaccagccg
     2401 cagcgccgcg aacgaccggt ggtggcgcca aactcggcgc cttcgcgcct gagggtttca
     2461 ccctcggctc cgtcgagttc cgtcgggaat gggccgctgc ccacccgtgt cgtgtaagcc
     2521 ttcgagatgc caatcacctg ctgaattttt tgcggaccga cgcctgaccc cgagcaggcg
     2581 ccaccggtga tggtgctcga agaggtgacg tacggatagg tcccatgatc gacatcgagc
     2641 aaggttccct gcgcgccttc aaagagcacc cgcttgccag cgtggatttg ctcatcgagc
     2701 agcacgccgg tattggtgac gtagctcttc agtatctccc ggaactcacc atagcggtcg
     2761 tgaatctcct cgaaatcgag cgccttttct ttgagaatcg ccttcaaata aaaattcttc
     2821 tcctgaatgt tgcgctcaag tttttcacgg aatgtgtctt cctccaggag atcgacgaaa
     2881 cgaataccga cacgggcgac tttgtcctcg taagcggggc caatcccgcg cccggtggtg
     2941 ccgatcttgc ccttgccgcg caatcgttcg cgggcgaggt cgatcgcctt gtgatacggc
     3001 atgatgacgt gcgcctgctc gctaatgcgc aactggtcag cgctggtgag atggccttga
     3061 gctttcagcg cttggatttc ttcgattagg acctcgggat cgacgacgac gccattgccg
     3121 ataatgcaca gtttgtccga atgtaacgcg ccggaaggaa ctagatgtaa aacagttttt
     3181 ttaccgtcga cgaccaaggt atggcccgcg ttgttgccgc cctggaagcg cacaacaata
     3241 tcggcttggc gggtgaagag gtcgacgacc ttgcctttgc cttcatcgcc ccattgggcg
     3301 ccgatgattg cgacgttgga catatttgca ttgtggattt tagattgccg attttggagt
     3361 ggaatcaaaa gtccaaaatc tagaatctaa aatccattca ca
//
LOCUS       DASULZ010000238         3085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_29968, whole genome shotgun sequence.
ACCESSION   DASULZ010000238 DASULZ010000000
VERSION     DASULZ010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29968"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2804
                     /gene="glnE"
                     /locus_tag="VFY96_04860"
     CDS             <1..2804
                     /gene="glnE"
                     /locus_tag="VFY96_04860"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEX5605822.1"
                     /translation="FLRQVSVEQKTRAEHVRELDPIIKATESFDECCAALRQYKQREY
                     LRIGARDLLPSVTLEETVRELTALAEASLDVAYRFCRAAVEMDYGILNLPDSDRENRF
                     TILGMGKLGGRELNFSSDIDVIFLFESDDGESSGGARGKLSPREFFSAVGKRIIQAMG
                     NVTEDGFVFRIDLRLRPMGNSGPLVQSVHSSMLYYESWGQCWERAALIKARPVAGSFE
                     LGAEFLKEIEPFVYRRYLDYTTVDELRHMKLRIENELLSAGEKERNLKLGYGGIREIE
                     FFTQALQLVNGGYVPEVRGPSTLPALKALARHKFISEKERDSLIDAYRFLRQAEHKVQ
                     IVQEKHAHSIPEGKEEERALARRLGYNAKGNFSERQLFWRDHRRHTRAVRRIFDHLFY
                     GAQKEMEAEGAAAARSVWHDLDRRESVTAELAQVGFKEPARAYNNLVAVRDGEIYAPP
                     STKRLRIMRTLGPALVDELTRSSDPDQALFNLAHFSHSIGGRTGFLTLLAENPKTLHV
                     LITLFAESQFLTELFLNRPELIDTLIRVDLTHVIKDRAEMLRETRSALSQCDDTESKL
                     NALRRYKNEEFIRIGLHDLGGAIDLIETLSQLSDLADASVEAVLDMTLAELSKKHGAI
                     PGGRFTIIGGGKMGGRELDYNSDLDLIFVYDAGDEAQSDGGLHGPLAAHDFYVRLGQK
                     LLSYLSAPTEEGILYKIDMQLRPSGKSGPLVSSLAAFREYHKSSSQLWERQALIKTRS
                     VAGDKTLGAEVETITAAFAYGKGLAPGDLGEIHHLRMRMERELAAENDTRFNLKKGRG
                     GLVDIEFITQMLQLAYGHRFPKLRQRSTLAALQGLYEQKVLKPNEHRLLSEGYLFLRK
                     LDHRLRLERDQSIDAFEADPGRLEGIALALGYGSANTAQRRTKDPGAKLLEEYRSKRE
                     RIRACYEHYFVSSQFDEIN"
     gene            2900..>3085
                     /locus_tag="VFY96_04865"
     CDS             2900..>3085
                     /locus_tag="VFY96_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605823.1"
                     /translation="MKTQLLKGIALASLVSLTATPLYAQTSAPSQPAAEEGVGEEAGY
                     GVGAALASIFYIPAKVTY"
BASE COUNT          725 a          837 c          861 g          662 t
ORIGIN      
        1 tttttcttcg ccaggtcagc gtcgaacaaa aaacccgagc cgagcacgtt agagagctcg
       61 acccgatcat caaagccaca gagagcttcg acgaatgctg cgcggcgcta cgtcaataca
      121 aacaacggga atatctccgc atcggcgcgc gcgacctgct gccgtcagtg accttggagg
      181 agaccgtgcg cgagctgacc gcattggccg aagccagcct tgacgtggcg tatcggtttt
      241 gtcgagcggc agtagaaatg gattacggta ttttaaattt gccggattcc gatcgagaaa
      301 atcgcttcac gatcctcggc atgggcaaac tgggcggtcg cgagctcaat ttcagctcgg
      361 atatcgacgt tatttttctt ttcgagagcg atgacggcga aagctccggt ggtgctcgcg
      421 gcaaattatc cccgagagag ttcttcagcg ctgtgggcaa acggattatt caggcgatgg
      481 gcaacgtgac ggaagacggc tttgttttcc gcatcgattt gcggctccgt ccgatgggca
      541 acagcggtcc gcttgtccaa tccgtacatt cttcgatgct ttactacgaa tcctggggac
      601 agtgctggga gcgcgccgcg ctcatcaaag cgcggccggt tgccggctcc ttcgaattgg
      661 gcgcagagtt cttgaaggag atcgaaccgt tcgtctatcg tcgttatctc gattacacca
      721 ccgtcgatga gctacgccat atgaagctgc gcatcgagaa cgagctgctg agcgccggcg
      781 agaaggagcg caacttgaag ctcggctatg gcggcatccg cgaaatcgaa ttcttcactc
      841 aggcgttgca gttggtgaac ggcggatatg tgccggaagt acgaggccca agcacactac
      901 cagctctgaa ggcgctggcc cgccataagt ttatttccga aaaggaacgg gattccttga
      961 ttgatgcgta tcgatttctg cgtcaggcag agcacaaggt acagatcgtt caggaaaagc
     1021 acgctcattc catacctgag ggaaaagaag aagagcgagc gttagcccgg cggctcggtt
     1081 ataacgccaa aggcaatttt agcgagcgac agctattttg gcgcgaccac cgccgccata
     1141 cccgggcggt cagaagaatt ttcgatcatc tgttttacgg cgcacaaaag gaaatggaag
     1201 cggagggcgc tgccgcagcg cgatcggtct ggcatgatct cgatcggcgg gaaagcgtta
     1261 cagcagaatt ggcccaagtc gggttcaaag agcctgcgcg cgcttacaac aatttggtag
     1321 cggtccgcga cggcgaaatc tatgcgcccc cgagcacaaa acgattgagg atcatgcgca
     1381 cccttggccc tgcgctcgtg gacgagctta ctcgatcgag cgatcccgat caggcgcttt
     1441 tcaatctcgc ccatttcagc catagcatcg gcgggcgcac gggatttctg actctgctcg
     1501 cggaaaatcc gaaaaccttg catgtactca ttactctttt tgccgagagc caatttttaa
     1561 ccgagctctt cctgaaccgt ccggagctga tcgatacttt gatacgcgtt gacttgacgc
     1621 acgtcataaa agatagagcc gaaatgctcc gcgaaacgcg atcagcattg agccaatgcg
     1681 acgacaccga aagcaagctc aacgccttgc gaagatacaa aaacgaagaa ttcattcgca
     1741 tcgggctgca cgatctcggc ggcgcaattg acctgatcga gacactatct cagctctcgg
     1801 atcttgccga cgcatcagtc gaggctgtgc tcgacatgac gctggccgaa ctcagcaaga
     1861 agcacggcgc gattcctggt ggccgcttca ctatcatcgg cggcggcaaa atgggtggcc
     1921 gggagctcga ttacaactcc gacctcgatc tgatttttgt ctacgatgcg ggcgatgagg
     1981 cgcaaagcga cggcgggttg catgggccgc ttgcggcgca cgacttttac gtccgactcg
     2041 gccagaagct tctttcttat ctgtcggcgc ctaccgagga aggcatcctc tacaaaatcg
     2101 acatgcagct tcggccttcc ggcaaatcgg ggcctttggt ttcctccctt gcggcttttc
     2161 gagaatatca caaatcgagc tcccaattat gggaacgaca ggcgctgatt aaaactcgtt
     2221 ccgtggcggg tgacaaaaca cttggcgcgg aggtcgagac gatcaccgcg gcttttgcct
     2281 acggcaaagg cttggcgccc ggcgaccttg gtgaaattca tcacttgcgc atgcgcatgg
     2341 agcgggagtt ggccgctgaa aacgacacgc gtttcaacct caagaaaggc cggggcgggc
     2401 tggtcgatat cgaattcatc acgcagatgt tgcaattggc ttacggccac cgcttcccca
     2461 aattacgcca gcgctccacc ttggcggcgt tacaaggatt gtacgagcaa aaagtgctga
     2521 aacccaatga acaccgtttg ctatcggagg gatatctttt tttacgaaaa ttggaccatc
     2581 ggctacgcct agagcgcgat cagtcaatcg atgcgtttga ggccgatccc ggcaggctcg
     2641 aaggcatcgc tctggccctt ggctatggca gcgcgaatac ggcacaacgt cgcacaaaag
     2701 accccggtgc aaaattactc gaggagtacc gctccaagcg ggaacgcatc cgggcctgct
     2761 acgaacacta ttttgtttcg tctcagttcg atgaaatcaa ctaagtagga gtgatccgag
     2821 cgagaagaaa ttttaccgca tccgttcttt ttaaaataga attattgaca gtagcacatt
     2881 cctgggatat cttcgccgca tgaaaacaca attgctgaag ggcattgccc tggcgtcatt
     2941 ggtttccctg acagcaactc cactttacgc ccagacatcg gccccgagcc aaccggccgc
     3001 tgaagagggc gtgggtgaag aagcgggcta tggggtggga gcggcgctcg cgagcatctt
     3061 ctatatcccg gctaaagtaa cgtat
//
LOCUS       DASULZ010000239         5231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1145959, whole genome shotgun sequence.
ACCESSION   DASULZ010000239 DASULZ010000000
VERSION     DASULZ010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145959"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1831
                     /locus_tag="VFY96_04870"
     CDS             <1..1831
                     /locus_tag="VFY96_04870"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5605824.1"
                     /translation="GGNIHGVYHASSGDIELGFSESDEVTENVYTLPSIQHGHIEPHA
                     VTAYWEPSGKLVVYTPCQTPSPLQEQLAKIFKLSLNRVRVIVPPVGGGYGGKNHARIE
                     PVVALLARKARRPVTWLLTREEVFLTGRRFGAVVKIKTGFKRDGRLWARKVDVYYDMG
                     AYALSGPANTKNASIIAGGPYNVPHRDYTTYAVYTNLPPAGPFRGVGASHVCWAYESE
                     MDEIARRTGIDPLELRLKNLIQEGERFITGEAMTSVGISECLKRTARAIGWRSKQEQS
                     NKTGILVRGKGLAVAIKSTSTPSTSAASVRLNSDGSAVLLTSSVEIGQGAHTLLAQIV
                     GDALGLPLERVTVSYPDTDVTPYDKSTSSSRTTFHMGRAAQIAAGQIRDQLLEAGARL
                     FEARIEDLELKELRLQVRGVPEKHLTFPQLFRTITGDTSGSLFGHHTLHTEGGVDSNT
                     GQGKGSSFWFYSAAGAEVEVDTETGKVRVLKLSAAVDVGKAIHPKQCELQNEGSALAG
                     LGSALFEEMRFDNGQPINATFLDYLLPSMHDHPGEFASVLVETPHPDGPFGAKGMGEA
                     ALPPMAPAIGNAVANALNGLRVRELPIKPEKIFTAVQAAKAGH"
     gene            1838..2626
                     /locus_tag="VFY96_04875"
     CDS             1838..2626
                     /locus_tag="VFY96_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605825.1"
                     /translation="MSTEICYWKHDIDKDAAFLCYDGKSVVCSRCCVEVCGKETPQWF
                     AACARAGHPTWPSIDRGRRVPTKLVCLESYWDNRLFHTTSVKGFFESMAPLHDPPLQL
                     AHRFVESQRGLAHYTAAPNGLLWTDPAAADAPIFYLAFHGRPTEVRSVLEFIDEQSLC
                     DTFKGFGIKPNLVYFAACSVLKGRNGRRFAEKFLKSSGCRAVIGYTTDVNWMLSLLTD
                     MLFLHRFYRDDHPWRNLRKIYDSVLDDFKPAQAIGWTLIENRRS"
     gene            2801..3460
                     /locus_tag="VFY96_04880"
     CDS             2801..3460
                     /locus_tag="VFY96_04880"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5605826.1"
                     /translation="MLKQSEFWSKIAEKYDRVVDLQIGSKTRSLVRERLRKEGPLGNV
                     AEFGCGTGYYTQVLVEKANSVTATDLSSGMIDVAKQRITAGNAKFQSEDCQHTSLPAA
                     AFDTAFISLVLHFTDPERTVAEMQRILKTGGTLIIANLDFNSLRGLDRARATIRILYH
                     GATGYRLKPPKRFITKMLTEKQLCDLLNRSGFKVLSSEVIKDQSHSSNLPIEYIKAIK
                     T"
     gene            3603..4031
                     /locus_tag="VFY96_04885"
     CDS             3603..4031
                     /locus_tag="VFY96_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5605827.1"
                     /translation="MKTVRDILEVKGRTVWSVSPGSSVFDALGLMAEKEIGALVVLDG
                     ARVAGIISERDYARKVILHGRASPTTRVEEIMTSHVAYTHLDESIEECMAIVTDKRIR
                     HLPVLQDGKLIGIISIGDLVKAIIADQKFMIEQLERYITS"
     gene            complement(4221..4589)
                     /locus_tag="VFY96_04890"
     CDS             complement(4221..4589)
                     /locus_tag="VFY96_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5605828.1"
                     /translation="MARIRHLAILTEDVEKLVRFYTTAFGLKIVHGVGTATYLSDGHI
                     NLAIIPIGPEREIEGPQLQVGINHFGFEVEDVAQVREACKELNASTNVDKRPPNREAE
                     YRVHDPDGNPIDLSKHSWPV"
     gene            complement(4910..>5231)
                     /locus_tag="VFY96_04895"
     CDS             complement(4910..>5231)
                     /locus_tag="VFY96_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NmrA family transcriptional regulator"
                     /protein_id="HEX5605829.1"
                     /translation="DGKVVRISPGLFQPIASDDVAAFVAEAALAAPRNGTVEIAGPER
                     APFDQIVARYLKAVGDPREVVRDADALYFGGRVEERSLVPLGEARLGRIGLDEWLRRS
                     QARA"
BASE COUNT         1171 a         1512 c         1435 g         1113 t
ORIGIN      
        1 tggcgggaat atccacggcg tgtaccacgc ctccagcggc gatattgagc taggctttag
       61 cgagtccgac gaagtcaccg aaaatgttta tacgctgccg tcgattcaac acggtcatat
      121 cgaaccgcac gcggttaccg cctactggga gccaagtggg aagctcgtcg tgtatacccc
      181 ctgtcaaaca ccctcgccgc tccaggagca attggcgaaa attttcaaac tgtcgctcaa
      241 tcgcgtccgg gtcattgtcc cgcctgtggg cggcggatac ggcggcaaaa accacgcacg
      301 gatcgaaccg gtagtagcgt tgcttgcgcg caaggcgcgc cggcccgtca cctggctact
      361 cacccgcgaa gaagtttttc tcaccggtcg ccgctttggc gcggtggtaa aaatcaaaac
      421 cggctttaag cgcgacggcc gcttgtgggc gcgcaaagtt gatgtctatt acgatatggg
      481 cgcctacgca ctgtcggggc ctgcgaatac aaaaaatgcc agtatcattg ccggcgggcc
      541 ttataacgtt ccgcatcgcg actacacgac ctatgccgtt tacaccaacc tacctccggc
      601 cggtcctttt cgcggcgtcg gcgcctccca cgtctgctgg gcgtacgagt ccgaaatgga
      661 tgagatcgcg cggcgaaccg gcatcgatcc gctggaactg cggctgaaga atttgattca
      721 ggaaggcgaa cggttcatca ccggcgaggc gatgacttca gtggggattt cggaatgcct
      781 caagcgaacg gcgagggcga tcggctggcg cagcaagcaa gagcagtcaa acaaaacggg
      841 aattcttgtg cgcggtaaag gcttggctgt ggcaatcaag agcacttcga cgccaagcac
      901 ctccgccgcg agcgttcgct tgaatagcga cggatcggca gttctgctca cgagcagcgt
      961 cgagatcggc caaggagcgc acactttgct cgcgcagatc gttggcgatg cgctcggatt
     1021 gccactcgag cgcgtcaccg taagttatcc agataccgat gtgacaccgt acgacaaatc
     1081 gacgagttcg agccgcacga cttttcacat ggggcgcgcc gcacaaatag cggccggtca
     1141 aattcgcgat cagctgctgg aagccggcgc gaggctgttc gaagcgcgaa tcgaagatct
     1201 cgagctcaaa gagcttcgcc tgcaagtcag aggggtgccg gagaaacacc ttacgtttcc
     1261 gcaactcttc cggacaatta ccggcgatac cagcggcagc ttgtttggcc atcacacgct
     1321 tcataccgaa ggcggcgtcg actcgaacac cggccagggc aaaggttcgt cattttggtt
     1381 ctattccgcc gcgggcgctg aagtcgaggt ggatactgag accggcaaag tgcgcgtgct
     1441 gaaacttagt gccgctgtcg acgtcggcaa agcgattcac ccgaaacaat gcgagctaca
     1501 gaacgaggga tccgccctcg ccggcctggg ctctgcatta ttcgaagaaa tgcgcttcga
     1561 caacggccag ccgattaacg ccacgtttct cgattacctg ctgccgtcga tgcacgatca
     1621 tcccggcgaa tttgcatcgg tcttggtcga gacgcctcat cccgatggcc cgttcggcgc
     1681 caaaggcatg ggcgaagccg cgcttccgcc gatggcgcca gcgatcggca atgccgtcgc
     1741 caatgcactg aacggcctgc gtgtgcgcga actgccgata aagccggaaa aaatctttac
     1801 cgcggtccaa gcagcaaaag cggggcactg agatttcatg tcgaccgaga tctgttattg
     1861 gaagcatgac attgacaagg acgcggcttt cctttgctac gatggcaaga gtgttgtatg
     1921 cagccgctgc tgtgtcgaag tctgcggcaa ggaaacgccg caatggttcg ccgcctgcgc
     1981 gcgtgccggc catccgacct ggcccagcat cgatcgcggc cggcgggttc cgacaaagct
     2041 cgtttgcctt gagtcctact gggacaaccg gctgttccac acaacttccg tgaagggctt
     2101 ttttgaatcg atggcgccgc tccacgatcc gcctttgcaa ctggcccatc gcttcgtcga
     2161 aagtcaacgc ggtctcgcac actacaccgc cgcgccgaac ggacttctct ggaccgatcc
     2221 ggccgctgcc gatgctccga tcttctatct cgccttccac ggccgcccca cagaagtgag
     2281 atcggtgctc gaattcatcg atgaacaatc gctttgcgac accttcaaag gcttcggcat
     2341 caaacccaat ctcgtctatt ttgctgcatg cagtgttctc aagggccgca acggccgccg
     2401 cttcgctgaa aaatttctca agtcttccgg gtgccgcgcg gttattggat acaccaccga
     2461 tgtgaattgg atgctcagcc tgttgaccga tatgttgttt cttcatcgct tctaccgtga
     2521 tgaccatcca tggcgcaatc tgcggaaaat ttatgactca gtgctcgatg attttaagcc
     2581 cgcgcaggca atcggttgga ccttgatcga aaaccgccgt tcatagaaac cgatcgatat
     2641 ccccggacga catagattgc cgttacactg catttttgcc aaccggattc ggtccaggac
     2701 agcgcgaccg cggatccagg agtttcgtag gggcgtatgg catacgccct ccggtgccga
     2761 tcgaataagc tggaaaattt caccgtcgag acattagacc ttgctaaagc aaagtgaatt
     2821 ctggtcgaag atcgcagaaa agtacgatcg tgtcgtagac ttacagatcg gctcaaagac
     2881 ccggtctcta gtccgggagc gactccgcaa agaaggaccg ctcggaaatg tggcagaatt
     2941 cggttgcggc acgggttact acacgcaagt cctcgttgag aaggcgaaca gtgtcaccgc
     3001 cacggatctc tcttcgggaa tgatcgatgt cgcaaagcaa cgaatcactg ccggcaacgc
     3061 aaaattccag agcgaagatt gccaacacac gtctttgcca gcggctgcgt tcgacacggc
     3121 cttcatcagt ctcgtacttc atttcaccga ccccgaaaga acagtggcgg aaatgcagcg
     3181 catactcaaa acgggcggca ccttgatcat cgcaaattta gattttaatt ccctgcgtgg
     3241 tctcgatcgc gctcgcgcca ccatccggat cttgtatcac ggcgccaccg gctacaggct
     3301 gaagccgccc aaacgattca tcactaagat gttgaccgaa aaacaactct gcgatctcct
     3361 gaaccgctcg ggatttaagg tactcagcag tgaggtgatc aaagatcaat cccactcatc
     3421 caacctgccc atcgaataca tcaaagcgat caaaacttga aagcgccgtt tcttggtttt
     3481 tgaaacccgc ctctcgatct gtcaaggatt attcagctca gcacgctggt tatggctttt
     3541 actcgtcgaa cgtttggttg tataaaattt cgcagcaaga ttccttgaga aggaggtctc
     3601 agatgaaaac ggtgcgagac atccttgaag tcaaaggccg cacggtttgg agcgtcagcc
     3661 ctggatcgtc ggtcttcgat gcgctcggtc tgatggccga aaaggaaatt ggcgctcttg
     3721 tggtgctgga tggagcgcgc gtcgccggga ttatctcgga gcgagactat gcccgtaaag
     3781 tcattcttca cggtcgcgcc tcgccgacaa cgcgggtcga agagatcatg accagccatg
     3841 tcgcctacac tcacctcgat gaatcgatcg aagaatgcat ggcgattgtg acggataaac
     3901 ggattcgcca tctaccggtg cttcaagatg ggaaattgat cggcatcatc tccatcggcg
     3961 atttggtgaa ggcaatcatt gccgatcaga agttcatgat tgaacagctc gagcgctaca
     4021 tcacgagctg agagcagaac cgggaatctt tgtaagcgat aaacctattt ccctgtttgc
     4081 aacggctgac tcagggtttt tgtaaataca ggtcgagcag tcgaaaatcc cgcctacccc
     4141 ctttttgcca aagaggggca gaaattgacg aaaaatagct cgcaatttcg tccggcgaaa
     4201 aagaagcttt cgattaccgg ctacaccggc cagctatgct tcgagagatc gatgggattg
     4261 ccgtcgggat catgaacgcg gtattccgcc tcgcgattcg gcggccgctt atcgacgttg
     4321 gtcgatgcgt tgagttcctt gcaggcctct cgaacctgag ccacatcttc gacctcgaaa
     4381 ccgaagtgat tgattcccac ctgcaactgc ggcccttcga tctcgcgctc cggtccgatc
     4441 ggtatgatcg caagattgat gtggccgtca gaaagataag tcgcggttcc aactccgtgg
     4501 acgatcttca atccgaatgc ggtcgtataa aacctgacca gcttctccac atcttcggtc
     4561 aaaatcgcca ggtgacggat gcgagccatg gattcctcct ttggtacggg cggacctccg
     4621 tgtccgcccg gctaaaacga gcatgtaggt cagcgtcggc gccattacag ctcgccgaag
     4681 caccttatac cattgtccca gccgcttctg catggcggcg tcgaacggct ccagggttta
     4741 agcgttggat tgaatgtgtc ccaatctatt caatgtttca cggcgctctc agccggccga
     4801 ttttaccgat tcgaatactt agtggcatat agtcttgccc aagttgtagc acagctgttc
     4861 cttatcggca tcgttttatg gagccctctc cttcggtggc ggagcagggt caggctctgg
     4921 cctgtgagcg gcggagccat tcgtcgagac cgatgcggcc gaggcgcgct tcgcccaacg
     4981 gcacgagcga gcgctcctcg acgcggccgc cgaagtacaa agcatcggcg tcacgcacga
     5041 cttcacgcgg gtcgccgacc gccttcagat aacgggcgac gatctggtcg aacggcgctc
     5101 tttccgggcc ggcaatctcg acggtgccgt ttcgcggcgc ggccagcgcc gcctcggcaa
     5161 cgaaagcggc aacatcgtcc gacgcaatag gctggaacag gccgggcgaa atcctgacga
     5221 cctttccatc c
//
LOCUS       DASULZ010000240         2583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_333321, whole genome shotgun sequence.
ACCESSION   DASULZ010000240 DASULZ010000000
VERSION     DASULZ010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333321"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..771)
                     /locus_tag="VFY96_04900"
     CDS             complement(64..771)
                     /locus_tag="VFY96_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX5605830.1"
                     /translation="MTIRAVIFDFFGTLVDDFVAATGQMTPDLATAVNAPHEQFIELW
                     RKTTSLRIDGTFQSVEAAIEHVCSTIGVEISAEQMKNAVELRMQQISGAFVPKPDAVE
                     TVRQLKDSGYKIGVLSNCSIEIPLLWAESPFSQLGDSAVFSSREHLKKPDPRIFDLAC
                     KRLDEIPHQCLYIADGENHELSVARSIGLHPVLIRNSFASRRKELFREATEWDGLAIS
                     SLSEVLNVLNAAAVRET"
     gene            complement(830..1459)
                     /locus_tag="VFY96_04905"
     CDS             complement(830..1459)
                     /locus_tag="VFY96_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX5605831.1"
                     /translation="MAPEKRRVHFSMLRSYQLADLITLANGAAGTSSVFAAMSYLTAP
                     EHWRIYLALALLPLALILDIADGKIARHHREQSLFGQELDSLADIVSFGVAPAALAYA
                     LGMRGAVDMVLLIFFVSCGISRLARYNVTAAELAGPAGKVRYYEGTPIPSSLILVALL
                     ALCVFIDRIGNELPLGVVDIAGAHWHPFSLVFFINGCAMISKTLRIPKL"
     gene            complement(1514..>2583)
                     /locus_tag="VFY96_04910"
     CDS             complement(1514..>2583)
                     /locus_tag="VFY96_04910"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX5605832.1"
                     /translation="TILAARQFGFKGEILALVEDPYHREPLILAGATGAYTPRHVLGA
                     ALAARASQKVSPTVAGIQHLGQKLQVGEARIMRDSVLGGKTLAEAEFGQHSGVTVIGQ
                     WIGGKLITPPTPEMRLEAGGILVLVGNAESLQRFMTLCAGARQLRRHGPFLIAGAGEV
                     GRKVAELLTDAGEETFLIDTQPGPTVNRVGNVLDTQLLKQAGVENVQAVILALNTDST
                     TLFATVIFKDLAPDVPVIARVNRAENVERIYAAGADFALSISQVSGQLLAWRLLGKES
                     VAVDPDLRVIKVSTRGLEKYHPGQSDIRVKTGCTVVAIERGEQLLVEFATDFHYEPDD
                     AVYICGSAEAARKFYEVFPQE"
BASE COUNT          585 a          730 c          716 g          552 t
ORIGIN      
        1 cggtgtacgc caagaactca tttcttttcg tgccgaaact ggttttgcgt aaccctcgat
       61 ggattacgtt tcacggacag cagcagcgtt tagaacgttc aatacttcag aaagcgacga
      121 tattgccaaa ccgtcccatt ctgtcgcttc acggaacagc tcttttcgcc ggctcgcgaa
      181 ggagtttcgg atcagcactg gatgaagccc gatactccga gccacgctta actcgtggtt
      241 ttctccatcg gcgatgtaaa gacattggtg gggaatttcg tcgaggcgtt tacacgcgag
      301 atcaaagata cgcgggtcgg gttttttgag atgctcgcgg ctggaaaaaa ccgccgagtc
      361 gcccagttga gaaaatgggg actcagccca caggagcgga atctcaatcg agcagttgct
      421 gaggacaccg attttgtaac cgctatcctt tagttgcctg accgtttcca cggcatccgg
      481 ttttggtaca aacgcgccgc tgatctgctg cattctaagt tccacggcgt ttttcatttg
      541 ctccgcgctt atctccacac cgatggtact gcaaacgtgt tctatagccg cttcgacgga
      601 ctgaaaggtg ccgtcgatac gcagactcgt ggtcttccgc cagagctcga taaactgctc
      661 atgcggcgca tttacggctg tcgcaagatc aggggtcatt tgaccggtcg ctgccacgaa
      721 atcgtcaact aacgttccga aaaaatcgaa aatgacggct cgaatcgtca cgcatgcacc
      781 cacaattcag tgatgctctt aatgtcacgc tgtatcacga gcaacggcgt tataactttg
      841 gaattcggag agttttgctg atcatggcac agccgttgat aaaaaaaacc agtgagaatg
      901 gatgccagtg agcgcccgcg atatcgacga caccgagcgg gagctcattt ccgatccgat
      961 cgataaatac gcacagggca agcagggcaa ccagaataag gctcgatggg attggcgttc
     1021 cttcgtagta tctcaccttg ccggccggac cagcgagctc ggcagcggtg acattgtagc
     1081 gcgccagtcg gctgatgccg cagctcacaa aaaaaatcag caaaaccata tccacagccc
     1141 cgcgcatccc aagggcgtaa gcgagggcgg cgggggcgac accaaagctt acgatgtcgg
     1201 ccagcgaatc cagttcctgc ccgaacagag actgctcgcg gtgatggcgc gcaattttgc
     1261 cgtcggcaat atcgagaatt agcgccagcg gtaacagcgc gagcgctaga taaatgcgcc
     1321 agtgctcggg ggctgtcaaa tagctcatcg cggcgaatac cgaggacgtt cctgccgcgc
     1381 cattggcaag cgtaatgagg tccgcgagct gatagctacg cagcatcgaa aaatggacac
     1441 gtcttttttc cggggccatg gctcctcatt ggttgtaaac gccactgcgg tcgtgagcgc
     1501 aacctgcgac ttgctattcc tgaggaaaaa cctcgtaaaa cttccgcgcc gcctcggcgc
     1561 tgccgcagat atagactgca tcgtccggtt cgtaatgaaa atcggtagca aattcgacga
     1621 gcaactgttc gccgcgctcg atggcgacaa ccgtacagcc tgttttgacg cgaatatcgg
     1681 attgcccggg gtgatatttt tccagcccgc gcgtcgatac tttgatgacg cggagatcgg
     1741 gatccacggc gacggactct ttgccgagca gtcgccaggc gaggagttgt ccggagactt
     1801 gactgattga aagcgcgaaa tctgcgccag cagcgtagat tcgctccacg ttttcagctc
     1861 ggttcactcg cgcaatgacc ggaacatcgg gagcgagatc tttgaaaata accgtggcga
     1921 agagcgtcgt ggaatcggta ttcagcgcca ggatcacggc ctggacattt tcaactcccg
     1981 cttgtttcaa gagttgggta tcgagcacgt tgccgactct attcacagtg gggcccggct
     2041 gcgtatcgat aagaaaagtt tcttcgcctg cgtcggtcaa caactcggcg actttgcgac
     2101 ccacctctcc agccccagcg atcaaaaacg gcccgtgccg gcgcaactgc cgagccccgg
     2161 cgcaaagggt catgaagcgc tgcagacttt cggcattgcc gacgagcacc aggataccgc
     2221 cagcttcgag acgcatctca ggcgtcggcg gcgtgatcaa cttgccaccg atccattgac
     2281 cgatgacggt tacgcccgaa tgctggccaa actcggcttc ggccagggtt ttaccaccga
     2341 gcacgctgtc gcgcatgatg cgcgcttcgc ccacctgaag tttctgaccc aggtgttgaa
     2401 tgccggctac cgtcggactg actttctgac tcgcgcgggc ggcgagcgct gcgccgagca
     2461 cgtgccgtgg cgtgtatgcg ccggtcgcac cggcaaggat cagcggctca cgatgatatg
     2521 gatcttcgac caatgccagg atctcgcctt taaagccaaa ctgacgtgct gccagaatcg
     2581 tcg
//
LOCUS       DASULZ010000241         2334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_638103, whole genome shotgun sequence.
ACCESSION   DASULZ010000241 DASULZ010000000
VERSION     DASULZ010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638103"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            441..1397
                     /locus_tag="VFY96_04915"
     CDS             441..1397
                     /locus_tag="VFY96_04915"
                     /inference="COORDINATES: protein motif:HMM:NF018047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HEX5605833.1"
                     /translation="MKRPALTCPHPSNAFVSRISSHSRYDIVGAFANFFPATDFPDYD
                     AFLNALGSCLKREQRTIVTIFHRVCRGSSSEDDVSSFVLKVYKYPFWPRIRTGFQISK
                     AEREFHNLRYLNRLGIGAAQAVAYGVDRDCLGLVRSCFVITRFVDNSMNLSQWSDELR
                     LNKNVEIKRLEQIFTELGQTFRRLHQARFFLFSPKSKNILVRQGSNRADELLLIDVPY
                     ARTLKWPVLASWARARDVGVLFANLTESLSEIAIELFYRAYLPDPLGLPANTVRRHVL
                     RQMRVKQNRTLLSRSVNKIKRRLMGKINQLIGFLILAEIAYG"
     gene            1390..2052
                     /locus_tag="VFY96_04920"
     CDS             1390..2052
                     /locus_tag="VFY96_04920"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5605834.1"
                     /translation="MDEQILARYNSDEGAKDYLQKFQRHWTERVNNWHEQRLIRRFLR
                     KVSSIDRNGLALDLPCGYGRLYSIVRELGLPVVEADWSFPLLAAARSIHASRYGTEPS
                     QNYVRATALAMPFKARAFEFVLSARLSHHIREHEQRLRHVREVLRVSGKWAMFTYFDT
                     ASVKNRAHEFRRRFRQKRAKWTLTLEEVRAVGQTEGFEVVQWAWISRFFSGHRYVLMR
                     RN"
BASE COUNT          517 a          578 c          620 g          619 t
ORIGIN      
        1 gcaccggaag cggttttgtt tttcgagccc attggctgtt ttccgctaaa aaatcgaacg
       61 gtttcttgta cgctcgcgag ttgcgacctt gcgctgagtc gctctcacca atcaaaccgg
      121 ggcttcatta aaaactgagc acttttaagt taatgtgcgg tgcgccggaa ccagaaactt
      181 ccaccaacca gttccgatga ggcctaggac agtcaccccg gtcttttttt ccatccgccg
      241 cgcgggagaa cggctcttgc ccgaaatgtc tgcagcaaac ggcagcggcc cagcccagct
      301 cttgggatca tctgctgagt ttgggcaggt cgggccacta ttcgttcggt ttgatccttg
      361 acaccaacat ttgtttttag cacaagggat gcaattctac ggggtcggta aatctttact
      421 gaataggcag ggtacaactc atgaaacggc cagcgctgac atgtccgcat ccctctaatg
      481 cctttgtctc gaggatatcc tctcattcgc gctatgatat cgtcggtgcc tttgctaatt
      541 tctttccagc cactgacttt cctgattacg acgctttttt gaatgcgctc gggagttgcc
      601 tgaagcggga gcagagaact atcgtgacta tctttcatcg cgtctgccga ggttcgagct
      661 ccgaagacga cgtgtcctcg tttgtactta aagtctacaa gtatcctttt tggccgcgta
      721 ttcggaccgg ctttcagatc tcaaaagctg aaagggaatt tcacaatctt cggtatctga
      781 atcgattggg aataggtgca gcgcaggcgg ttgcttatgg tgtggatcgc gactgcctcg
      841 gattagtgcg gtcctgtttc gtgatcactc gatttgtgga caactcgatg aatttatccc
      901 agtggtctga cgagctacga ctcaataaga acgtcgaaat aaagagatta gagcaaattt
      961 ttacggagct cgggcagact ttccgccgcc tgcaccaggc gcgttttttt cttttttccc
     1021 ctaagtcaaa gaatattctc gttcgccaag gttcgaatcg agccgatgag ctattgttga
     1081 tcgatgttcc ttacgcgcga acgctaaagt ggccggtgct tgcgagttgg gcgcgagctc
     1141 gggatgttgg agtgctcttt gccaatctca cggagtcgct atcggagatc gcaatcgagt
     1201 tgttttaccg cgcctatttg cccgacccct tagggttgcc tgcgaacaca gtcaggcgtc
     1261 atgtgctgag gcaaatgcgg gtgaaacaaa accggacctt actctcgcga tccgtaaaca
     1321 agatcaagcg ccgactaatg ggcaaaatca atcaactcat cggtttcctt atactagcgg
     1381 aaattgctta tggatgaaca gattctcgcg cgctataaca gcgacgaagg cgcgaaggat
     1441 tacctgcaaa aattccaacg gcattggaca gaacgcgtta acaactggca tgagcagcgt
     1501 ttgatccggc gatttctccg caaggtctca tccatcgatc gaaacggttt ggccctagat
     1561 ctgccgtgtg gctacggccg tctttattca atcgttcgtg aattgggact acccgtagta
     1621 gaggcggatt ggagctttcc cctgctcgct gccgcgagaa gcattcacgc aagtcgctac
     1681 ggcaccgagc catcgcaaaa ctacgtgcgt gcgacagctc ttgccatgcc atttaaagcg
     1741 cgtgccttcg agttcgttct ttctgcgaga ctctctcatc acatcaggga acacgaacaa
     1801 cgattgcggc atgtacgtga ggttttacgc gttagcggaa aatgggcaat gtttacttac
     1861 ttcgatacgg cgtccgtgaa gaatcgggct cacgagtttc gcaggcgatt tcgccaaaaa
     1921 cgagcaaagt ggacgttaac cttggaggaa gttcgagcgg tcggccagac cgaaggtttc
     1981 gaagtcgttc aatgggcatg gatttcgcgt tttttttcag gtcatcggta tgtattgatg
     2041 cggcggaatt gatcttgagg gcgtgacgct gttcggcaaa agtaccgcct gcgcgattga
     2101 gggttctgtt tgctgcgggc gccaagatgc tcttgtaatt ggcatggcgt tgggctccgg
     2161 atatcgttca tccgatcatg atgtaagtag ataaataccc ctcactgttc cgctttggta
     2221 tttactactg gcggaaactc tgccagcctt tagcttggca gtactaagtt cagggtgggc
     2281 aaatttccgg cgttgtctgc gggattgcga gagacgcgat tgacagctgg gtac
//
LOCUS       DASULZ010000242         4354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_30187, whole genome shotgun sequence.
ACCESSION   DASULZ010000242 DASULZ010000000
VERSION     DASULZ010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30187"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..3336
                     /locus_tag="VFY96_04925"
     CDS             178..3336
                     /locus_tag="VFY96_04925"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEX5605835.1"
                     /translation="MSLYVPLWCKSNFSFLEGASHPDELVDEASRFGLPALALTDRDG
                     LYGIVRAHVKARELGLKIIVGSEITIDDGSIMVLLAQDRAGYANLCRLISKGRLRSEK
                     GESSVKWDEVCAHAAGLIALWGGKYGDLDFGFRRNDAKRQSLRDDRSLSVYDVNVDDI
                     ASNLRDAFGDRLYVMLARHRREEEVVHESEWRERARRFGIPWIAANEVLYHTPARRRL
                     QDVLTAIRHGVPVASCGRRLKPNAEYALKPPYAFAKLFDDDPAAVARTLEIAERCSFS
                     LSEIRYRYPSEQLPDGTTSMEWLRKQTFNGARRRFEGDIPSEIIRQIEKELEIIEELD
                     YPGYFLTMWEIVEFCRAHDILCQGRGSAANSVVCYCLGITAVDPKSIGLLFERFISKE
                     RAEPPDIDLDIEHDRREEVIQHVYQKYGRERAAMVANVVRYRSRSALRDVGKALGLSE
                     TALDRAAKFLSSYEPIRSEALAQMGIHSAAGLHEHLIDLANEILDFPRHLSIHPGGFL
                     LGHEPVHDLVPIENATMPDRTVIQWDKIDIEDLGLFKVDLLGLGGLTQLDLCFKLLRE
                     HRALEYSMATIPKGDQATFDMICKSDTIGVFQIESRAQMAMLPRLKPKNFYDLVIEIS
                     IVRPGPITGGMVHPYLRRRQNKEEVTYPHDLLRPVLERTLGVPLFQEQVMRLAMVVAD
                     YTPGEADQLRRDMAAWHRSGRMERHRERLITRMQAKGIAVEFAERVFEQIRGFGEYGF
                     PESHAASFALIAYATAWLKCHYPAEFTCALLNAQPMGFYMPATIVEDAKRHQIVMRSL
                     DVRISDWDCTLEPCAESNGGFAVCMGLRYVKGLGEREGESIVEARRGAQFRSLQDFVR
                     RTHLDEGSLSALAEAGAFDGLDVDRRTALWDIRRFARTEKESLSLPARESKPGFAPLT
                     DLEEVNWDYRRTSHSARRHPLEPMRASLIRHHLPDARTVASMKNRTRIRYAGLVICRQ
                     RPGTAGGVVFMTLEDETGFVNVVVWESVFQRYSVLAKTASFLGITGTLQVEDGIVHLV
                     AEELWEPKIALRPASAGARDFH"
     gene            complement(3565..>4354)
                     /locus_tag="VFY96_04930"
     CDS             complement(3565..>4354)
                     /locus_tag="VFY96_04930"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5605836.1"
                     /translation="CMARAYNDWLYHKFMKASPRLVGVALLPIQDISEAVKELRRAVN
                     ELGMVGAVLPAVAPGGRLYSGPEFYPLWEEAQRLDVPVSTHGGLSMPALGLDMVNNFT
                     IGHTLEHPFAQIRQFVAMMFEGVFELFPNFRFGCIECGVGWVPYMMDRMDEEQERKGH
                     YSPRCKLKPSEYVKKGNIFFAVEVEESALPLASQVANENALIWASDYPHERDQRDFSQ
                     DIPNLINRQDVSDELKRKIFWDNPLRFYPRLKARVEKADSRAVA"
BASE COUNT          917 a         1198 c         1283 g          956 t
ORIGIN      
        1 gcgttagtgg aacatgggac tctcgcatgc aatctgaacc ccgagcgatg acgatttttc
       61 actctgaaac ttgcttccat cttctacatt aaagtcttaa gcagcagcat tcttctgagc
      121 gctatcatat gagtaaacgc gctgagcgca cttcacgaaa tcagcgtatc ggttgttatg
      181 tctctctacg tgccactctg gtgcaagagt aatttttcct tcctcgaagg cgcgagccat
      241 ccggatgagc tggtggacga agcttctcgg ttcgggctgc cggctttggc attgaccgat
      301 cgcgacggtc tttacggcat cgtccgggcg cacgtcaaag cgcgcgaact gggtctgaaa
      361 atcatcgtgg gttcggagat aaccattgac gatggttcga taatggtgct cttggcgcag
      421 gaccgcgccg gatatgcgaa tctctgccgg ttaatcagca agggccgatt acgttcggaa
      481 aaaggggaga gctcggtcaa atgggatgaa gtgtgtgcgc acgctgcggg attgattgcg
      541 ctatggggcg gaaaatacgg agacctggat ttcggctttc gccggaatga cgcaaagagg
      601 cagtctcttc gagatgatcg gagtctatca gtttatgacg ttaacgtcga tgatattgcg
      661 agtaacctcc gcgatgcttt cggtgatcgg ctttatgtca tgctggcgcg gcaccgacgc
      721 gaagaggaag tcgttcacga atcagaatgg cgcgagcgag cgcggcgctt tggcatccca
      781 tggattgccg ccaatgaagt gctttatcac acgcccgcac gacgccggct gcaagatgta
      841 ttgacggcga ttcgccacgg cgttccggtc gcgtcttgcg gccggcgatt aaagcccaat
      901 gcggaatacg cattgaagcc gccttacgct ttcgcaaagc tcttcgatga cgatcctgcg
      961 gcggtggcgc gcacgctcga aatcgccgag cggtgcagtt tttcgctcag cgaaatccgt
     1021 tatcgctatc cgtcggagca gttacccgac ggcacgacat cgatggagtg gttgcgaaag
     1081 caaacgttca acggtgcgcg ccggcgtttt gaaggggaca ttccgagcga gattatcagg
     1141 caaatcgaaa aggagctgga aatcatcgag gagctcgatt atcccggtta ctttctcacc
     1201 atgtgggaaa tcgtcgagtt ttgccgcgct cacgacatcc tctgtcaggg acgcggttcg
     1261 gcggcgaatt ccgtcgtttg ttactgcctc ggtattactg ccgtcgatcc gaaaagcatc
     1321 ggattattgt tcgagcgctt tatttccaag gaacgcgccg aaccgccgga catcgatctc
     1381 gatatcgaac acgatcggcg cgaggaagtg atccaacacg tttatcaaaa atatggtcgc
     1441 gagcgcgccg ccatggtcgc caatgtggtg cgctatcgct cgcgttcggc gctgcgcgac
     1501 gtcggcaagg ctctggggct ttctgaaacc gccctcgatc gcgcggccaa atttctctcg
     1561 tcctatgaac cgattcgctc cgaggcgctg gcgcagatgg ggatccattc ggcggcaggt
     1621 ctgcacgagc atttgatcga cctggcgaac gaaattctgg attttccgcg tcatctttcg
     1681 atccatcccg gcggttttct actcggccac gagccggttc acgatctggt gccgatcgaa
     1741 aatgccacta tgccggatcg cacagtgatc cagtgggata agatcgacat cgaagatctc
     1801 ggtctattca aagtggattt actgggtctc ggcggtctca cccagctcga cctctgcttc
     1861 aagcttttgc gcgagcaccg cgcgctggaa tattcgatgg cgacgattcc gaagggtgat
     1921 caagcgacgt ttgatatgat ctgcaagagc gacactatcg gcgtatttca gatcgagagc
     1981 cgcgcgcaaa tggcgatgct gccgcggctc aagccaaaaa atttttacga tctagtgatc
     2041 gagatcagta tcgttcgtcc cgggccgatc accggcggca tggtacatcc ctatctgcgc
     2101 cgacggcaga acaaagagga agtaacttat ccccatgatc tgttgcggcc ggtgctcgag
     2161 agaaccctcg gtgtgccgct gtttcaggaa caagtgatgc ggctggcgat ggtggtggcc
     2221 gattacactc ccggagaagc cgatcagctg cgccgcgata tggcggcctg gcatcgcagc
     2281 ggacgaatgg agcgccatcg cgaacgactg atcacgcgca tgcaggcgaa gggtattgcc
     2341 gtggaattcg ccgagcgcgt attcgagcaa attcgcggat tcggcgaata cggttttccc
     2401 gaaagccatg cggcgagctt cgccctgatt gcctatgcca ccgcttggct caagtgccat
     2461 tatccggcgg aattcacctg cgcgctgctg aacgcccagc cgatgggatt ttatatgccg
     2521 gcgacgatcg tcgaggacgc caaacggcac cagatcgtga tgcgatcact cgatgttcgg
     2581 atcagcgact gggattgcac cctggaaccc tgcgcggaaa gcaacggcgg cttcgccgtg
     2641 tgcatgggac tccgatatgt gaaagggctc ggcgaaaggg agggagaaag tatcgtcgaa
     2701 gcacgtcgcg gcgcgcagtt tcgctcactc caggattttg tgcggcgcac gcatttggat
     2761 gaaggatcgc tcagtgcgct cgccgaagcg ggagccttcg acggtctcga cgtcgatcgc
     2821 cgcacggcgc tatgggacat tcgtcgcttc gcgcgcaccg aaaaagaatc tttgtcgcta
     2881 cccgcgcgcg aatccaaacc gggatttgcg cctttgacgg atctcgagga agtgaattgg
     2941 gattatcgcc gcacgtcgca cagcgcgcgg cgccatccgc tcgagccgat gcgcgcgagc
     3001 ttgatacgcc atcacttgcc ggatgcgcgc accgtggcat cgatgaaaaa tcgcaccaga
     3061 attcgttatg ccggcctggt gatctgccgg cagcgccccg gcacggcagg cggcgtcgtc
     3121 ttcatgacgt tggaggatga aaccggtttc gtgaacgtgg ttgtgtggga gagcgtcttc
     3181 cagcgctatt cagttttggc caagactgca agcttcctcg gcatcaccgg caccctgcag
     3241 gtcgaagacg gcatcgtcca tctggtagcg gaagaattgt gggaaccgaa gattgcgttg
     3301 aggccagcaa gcgccggtgc gcgcgatttt cactaagaga atcaccacag agtaattcat
     3361 gtagcaatcg gtacaacaca gcgcatgaga atgagccgta gtggttcgga ccaacaccga
     3421 caaaatcccc gatggccctt tggcgagggg gcggggccgt aggaccgggg gattttgcct
     3481 ctaatcggaa ttttcttaag cggcgcagag gacacagaga tcgttcctct gtgaactcag
     3541 tgtctccgca gtgtagtttc tttcttacgc gacggctcgg ctgtcggctt tctcgacgcg
     3601 cgctttcaag cgcggataga agcgtagcgg gttgtcccag aaaatttttc gcttgagctc
     3661 gtcactgaca tcttgacgat tgatcaggtt cgggatgtct tggctgaagt cgcgctggtc
     3721 gcgctcgtgc gggtagtcgg aagcccagat cagggcgttt tcattcgcga cttgtgatgc
     3781 gaggggcagg gccgattcct cgacctcgac ggcaaagaat atattgcctt tcttgacgta
     3841 ttcgctgggc ttgagcttgc agcgcggcga gtagtggcct ttgcgttcct gctcctcgtc
     3901 catgcggtcc atcatgtagg gaacccagcc gacgccgcac tcgatgcaac cgaatctaaa
     3961 attgggaaac aattcaaaga cgccttcgaa catcatcgcg acgaattgac ggatctgcgc
     4021 gaacggatgc tcgagcgtgt ggccgatggt gaaattgttc accatatcca agcccagcgc
     4081 cggcatgctc aaccctccgt gggtggaaac cggcacatcc aacctctgcg cctcctccca
     4141 taacggataa aattccggcc cgctgtaaag ccttccgccc ggcgccaccg ctggcaatac
     4201 cgcgccgacc atccccagct cgttcacggc tctgcgcaat tccttcacgg cttcggaaat
     4261 atcctgaatc ggcagcaacg cgacgccgac cagacgggga ctcgctttca tgaatttgtg
     4321 ataaagccaa tcgttgtagg cgcgcgccat gcag
//
LOCUS       DASULZ010000243         6622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1349236, whole genome shotgun sequence.
ACCESSION   DASULZ010000243 DASULZ010000000
VERSION     DASULZ010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6622
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349236"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="VFY96_04935"
     CDS             complement(<1..965)
                     /locus_tag="VFY96_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5605837.1"
                     /translation="MKVEAEVGAIGYAPRTRASGVGIFAGGILTLLVIMVGVPLLMVV
                     LMSLRTGFPGEGGPLTLTNFITVYSAPSTYKILFNTLLFAFGTVIVGLAFAVPLVWLL
                     NRTDIPLKNTIYVLMTVGILIPVFLRTIAWILLLSPRIGLVNQWAMQLFNLDQPIISL
                     YNIPGMAFVQGVSFVPSAFFMLSAAYRTMDPALEEAAYTSGIGKLQTFLRINVPITWP
                     AIAGVMVYLFMSALAVFEVPAIIGFPARILVLSASIFTATNPPTGLPNYGIAGAYGAV
                     MLLAGLILAYFYVRLVQHGKKYTVITGRGYRPRQIALGKWKWAALA"
     gene            complement(1263..2291)
                     /locus_tag="VFY96_04940"
     CDS             complement(1263..2291)
                     /locus_tag="VFY96_04940"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5605838.1"
                     /translation="MKKFLVQAWACGLLLVFCTAAVAAPIDDVIKAAKQEGTIEFYAP
                     STLTPQGAQAIGAAFNKKYGLNVKLQYSPSGNMTRDIGKVVGLGASGVPPEWDLMVVT
                     DAHHATLWVKKMHQPFDYKSIGVDPKAVQYDNGSVSFANQFVLPAYNKQLVPAKDVPK
                     TWDDLLNPKWKGKLGVSSATHHMARLAAGPWGEEKATNFVKALTKQDLILGRLGEIYN
                     RLLLGEIAVAVTLSDSEIKSEKNKTAPVVFAEAVSPLIAPAYHAGVPKNAPHPKVAHL
                     MAAYLTTPEAQKIWEKYSGQSSAFIAGTSAYKYVQGKDVVYMSQDQAVTVDRLAREYG
                     KIFGFTGL"
     gene            complement(2385..3575)
                     /locus_tag="VFY96_04945"
     CDS             complement(2385..3575)
                     /locus_tag="VFY96_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605839.1"
                     /translation="MTVADIQAQSENWNLRVIGHHDLNGHGDGMQLLKQGRYVYLAHL
                     GTSPMALSVIDVADPTAPQVVTQMPHAPNTHAHKVQIVGDVLIQNQERPYFDKTVPAS
                     VPNEAGIKVYSIEKPTEPREIGYLKLPGKGVHRMWFTDGKYAHVGAMLPGIKERAYLI
                     ADLSDPTNPKEAGRWWIPGTKEGEETPPDWTPFPGEHFHVHGAIPEGDRSYVALVDAG
                     MAIVDISDIAKPKTISHIDWSPPFGGYAHTTLPLPGRKLVVAVDESVKYDCNEGEKRV
                     WLIDIREERNPVIVSSCPVPEGDFCKRGLRFGPHNVHEMRPGSYQNENLIFVTYYNAG
                     LRVFDIRNKFRPEEVGFFIPPAPEEQKAPMFNDLYVDTEGLIYVSDRIKGGLYILEYT
                     GPKV"
     gene            3792..4769
                     /locus_tag="VFY96_04950"
     CDS             3792..4769
                     /locus_tag="VFY96_04950"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605840.1"
                     /translation="MKRLLAFAILALLAPTTDVHAQLKRIAAGYSAVSATQTGFYIAK
                     DAKLFEKYGLFVDPVYVPGGSRMAQAIIAGEFPLALAGGNIVNVNLAGGDIVIIGGVV
                     NVPSFYLFVQPAIKRNEDLKGKALGVTRYGASTDVSLRACLKKYALEPDRDVKIIQMG
                     GQPEIVAGMQAGVVQGGILSSPADFRAKKSGFVMLANFAKEGIDYPTTSVVSTRSTIK
                     KDRDTVKRFLMAYSEAVDLLFRDKELAMKVIGKWTRTQDRDTLESSYEYATNFIERPP
                     RLPFKATENIVASMAETDPRAKDHKAEEFLDPSIYNELEKSGFFKSLGR"
     gene            4905..5750
                     /locus_tag="VFY96_04955"
     CDS             4905..5750
                     /locus_tag="VFY96_04955"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol ring-cleavage dioxygenase"
                     /protein_id="HEX5605841.1"
                     /translation="MAEIVSIIGITHNPFMPRLFQQPQQPPGAAQVKERIAMMRQKLA
                     DANPDVLVCIGNDHLHQFFMDNMPAFMIGKMEQYNGTFYDEEREFGLPRCKLPGDLDV
                     ADAIMEGAFDRGVDFSYSNELTIDHSIVVPMMFVRPAMDIPIVPILTNCIAPPMPRAR
                     RYFEVGKALRDAIDSLPATKRIGVLVSGHLSLEIGGPKQFERHLTDPNFDAAAVGWIM
                     NGDIDGAATGCTPEKMIAAGNMTSGYLNFLMMMGLANATKPSHAEGLDAGFPAIPFFS
                     WDKQR"
     gene            6022..6294
                     /locus_tag="VFY96_04960"
     CDS             6022..6294
                     /locus_tag="VFY96_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEX5605842.1"
                     /translation="MDFRVLATIFTSVFIAELGDKTQLATMLFAADKQVSKLTVFIGA
                     SLALIVTSAIGVIAGSAISNYVSEKTFHYLAGAGFIAIGVWTLVKA"
     gene            6304..6615
                     /locus_tag="VFY96_04965"
     CDS             6304..6615
                     /locus_tag="VFY96_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605843.1"
                     /translation="MSKYLMNKLIHLINMNEEAEKEYKARPREFVEQWEQTQTLKLEP
                     EEREALATKDYGKLYALGAHPFTLWSFTEAVWVHERPREELVADYKAKAAQAGYPSFK
                     T"
BASE COUNT         1510 a         1860 c         1759 g         1493 t
ORIGIN      
        1 gccaacgcgg cccatttcca tttccctagc gcgatctgac gtggccgata gccgcggccc
       61 gtgatcacgg tatatttttt tccatgctgc accaggcgca cgtagaagta agcgagaatc
      121 aggccggcga gcagcatcac agcgccgtag gctccggcaa tcccgtaatt gggcagccct
      181 gtcggcggat tggttgccgt aaatatggaa gcgctgagca cgagaatgcg cgccggaaag
      241 ccgatgatcg ccggcacttc aaaaaccgcc agcgccgaca tgaataagta gaccatgact
      301 ccggcaatgg caggccatgt gatcggcaca ttgatgcgca gaaaagtctg cagtttgccg
      361 atgcccgacg tgtatgcggc ttcttcgagg gccgggtcca tggttcgata cgccgcagag
      421 agcatgaaga aggccgacgg cacgaatgaa actccctgca caaatgccat tcccggaata
      481 ttgtagagac tgatgatcgg ctgatcgaga ttgaagagtt gcatcgccca ttgattgacc
      541 aaaccgatgc gcgggctgag cagaagaatc caggcgatgg tacgcagaaa aaccggaatc
      601 aaaattccca ccgtcattaa gacgtagatg gtattcttga gcggaatgtc ggtgcggttg
      661 agcaaccaaa ccagtggcac cgcgaaagcc aaaccgacga taacggtgcc gaaagcgaag
      721 agcagcgtat tgaaaaggat cttgtaagtg cttggggcgc tatagacggt aataaaattc
      781 gtcagggtga gcggtccgcc ttcgcccggg aatccggtac gcaagctcat gagcactacc
      841 atcaggagcg gcacaccgac cataatcacg agcagggtca aaattccgcc ggcgaatatc
      901 ccaactccgc tcgccctagt ccgtggcgca tacccgatag cgcctacttc cgcttcaact
      961 ttcatctgag gttagtccgt tggtgtcatc attcatgacg aaaacgatcg cttgcggcca
     1021 acgcaagaat cctggtgcga gcttccctgt catttcgacc gaagggagaa atccttcccc
     1081 tttttccttg ttcttcaaga cactaagaaa caagaaagat ttctcgctca cgcgaaatga
     1141 caatttccaa gctatgtacg gcgggcgagc tcgccacatc gtggccggag agagcgcagc
     1201 gtaaccgcgt taacccagat cgcctctcct aaaatctttg tcctcggtgc tgaaattggc
     1261 ttctacaatc cggtaaatcc gaatatcttg ccgtactcgc gcgcgagccg gtccacggtg
     1321 acggcttggt cctgggacat gtacacaacg tcttttccct gcacgtactt gtacgccgac
     1381 gttcccgcaa tgaatgccga gctttgaccg gagtattttt cccagatctt ttgcgcttcc
     1441 ggcgtggtca aataggccgc catgagatga gcgaccttcg gatggggagc gttcttggga
     1501 acgccggcgt gataggcggg cgcgatcagc gggctcacag cttcggcaaa taccactggc
     1561 gcagttttat tcttttccga tttgatttcg ctgtcgctca aggtcaccgc gacggcgatt
     1621 tcgccgagca acaatcgatt gtagatctct cccaggcggc caagaatcaa gtcctgtttc
     1681 gtgagcgcct tgacgaaatt ggtcgctttt tcctcgcccc acggcccagc cgcgagccgc
     1741 gccatatgat gcgtcgcgct cgagacgccg agtttgccct tccatttggg gttcaacaaa
     1801 tcgtcccaag ttttcggcac atccttggcc ggcacgagct gcttgttgta agccggcaag
     1861 acaaactgat tggcgaaact caccgaaccg ttgtcgtatt gcacggcctt cgggtcgacg
     1921 ccaatgctct tgtagtcgaa tggctggtgc atctttttga cccacaaggt cgcatgatgc
     1981 gcgtcggtga cgaccatcaa atcccattcc ggcggcacgc cgctcgcgcc gagcccgaca
     2041 accttaccga tatcgcgggt catgttacct gaaggactgt actgaagctt gacgttgagt
     2101 ccgtattttt tattgaacgc ggcgccgatc gcttgcgccc cttgcggcgt cagagtcgag
     2161 ggggcgtaga attcgatcgt accttcttgc ttcgccgctt taataacatc gtcgatcggc
     2221 gccgcgacgg ccgccgtgca gaaaaccagc aggagcccgc aagcccaggc ttggaccaaa
     2281 aacttcttca taattgcctc ctcaatatca gcacacagcg ccgtgatgtt cgcaaccggc
     2341 caaactcgat ccgtggatcg caattcgaca ctacgatccg tcagttaaac cttcggtccg
     2401 gtatattcca atatgtacaa gccgcccttg attcgatcgc tcacataaat caatccttcc
     2461 gtatcaacat aaagatcgtt gaacatcggc gctttctgct cttccggcgc gggcggtatg
     2521 aaaaaaccga cctcttccgg acgaaattta tttcggatat cgaacacgcg cagtcctgcg
     2581 ttgtaataag tcacgaatat aagattctcg ttttggtaag agcctgggcg catttcatgc
     2641 acattatggg gtccgaaacg cagtccgcgt ttgcagaaat cgccttccgg caccggacaa
     2701 gagctgacga tcacgggatt tctttcttct cgaatatcga tcaaccagac gcgcttttca
     2761 ccttcgttgc agtcgtattt caccgactca tccacggcga caactagttt tctgcccggc
     2821 agcggcaagg tcgtatgcgc atagccgccg aacggcggac tccaatcgat gtgactgatg
     2881 gttttgggct tggcgatatc cgagatgtcg acaatcgcca tgccggcgtc caccagcgcc
     2941 acgtaggatc ggtcgccctc cggaatcgcg ccgtgcacgt gaaagtgctc tcccggaaac
     3001 ggcgtccaat ccggtggggt ctcttctcct tctttggtac ccggaatcca ccaccgtccg
     3061 gcctctttgg ggttcgtggg atcggaaaga tccgcgatca aataggcgcg ctctttaatg
     3121 cctggtagca tcgcgccaac atgcgcatac ttgccgtcgg taaaccacat acgatgcacg
     3181 cccttgccgg gaagcttcaa gtagccaatc tcccgcggct ctgtcggttt ttcgatgctg
     3241 tatactttta ttcccgcttc attgggtacg ctcgctggca ctgttttgtc gaagtacggc
     3301 ctttcctgat tctgaattag tacatcgccg acgatttgca ccttgtgcgc gtgcgtgttc
     3361 ggcgcgtgcg gcatttgcgt gaccacctgc ggcgccgtcg gatcggccac gtcgataacg
     3421 ctcagcgcca tcggcgacgt gccgagatgg gcgagataga cataacgccc ctgcttgagc
     3481 aattgcattc catcgccgtg accgttgaga tcgtggtggc cgatcacccg caggttccag
     3541 ttctcactct gcgcctgaat atccgcgacc gtcataggga gccctcaatt cgtgaaatct
     3601 aaatacgctt tcctcacact gcgcttggtt ttcttatccc tacgcagtac ctcagtcaac
     3661 ggccgaacta acatttgagt acggcacaac agggagtttc ggactttttc cttgctggtg
     3721 tgggttggcc aaggcaaact tatgtggtag taagccgcca gtttactgga gatcgacgaa
     3781 ggagggacgg catgaaacga ctgcttgctt ttgcaatcct ggcgctactt gctcctacta
     3841 ctgacgtcca tgcgcaactc aagcgcatcg ccgccggtta cagcgcggtc agcgcaacac
     3901 aaacgggctt ttacatcgcc aaggacgcca agcttttcga aaaatatggc ctcttcgtcg
     3961 atccggttta tgttcccggc ggcagccgca tggcgcaggc gatcatcgcg ggagagttcc
     4021 cgctggccct ggccggcggc aatatcgtta acgtcaatct cgccggcggc gatattgtca
     4081 tcatcggcgg tgtcgtcaat gttccatcct tttatctgtt tgttcagccc gcgatcaaac
     4141 ggaacgaaga tttgaaagga aaggcgctcg gcgttacccg ctatggcgcc tccaccgacg
     4201 tgagcctgcg cgcctgttta aaaaaatatg ctttggaacc tgatcgcgat gtcaaaatca
     4261 ttcaaatggg gggtcagccg gagatcgttg ccggtatgca ggccggagtc gtccagggag
     4321 gaatcctgag ttcgcccgca gacttccggg caaagaaatc ggggttcgtg atgctcgcca
     4381 atttcgcgaa ggaagggatc gactatccga ccacgagcgt ggtttcgact cgttcgacta
     4441 ttaaaaaaga tcgcgacacc gtcaagcgct ttctgatggc ctacagtgag gcggtcgatc
     4501 ttctgtttcg cgataaagag ttggccatga aggtcatcgg caagtggacc cgtacgcagg
     4561 atcgcgacac tttggagtct tcctatgagt acgccaccaa tttcatcgag cggccgccga
     4621 gactgccgtt caaagcaacg gaaaatattg tcgcgtccat ggccgaaacc gatccgcggg
     4681 caaaggacca caaggccgag gaattcctgg atccttccat ttacaatgaa ttggagaagt
     4741 ccggattttt caaatcgctg ggccgatagc acggcaaacg gcagagcttc aggcaagaca
     4801 tcgcgagcgg ccgggaattc gttttgcacg cctttggtaa gcacgaccat ttacgctctc
     4861 aaatgggcgt gctaaacgga aaatcacaaa tcgaggaggc gaatatggca gagatcgtct
     4921 cgatcatcgg catcacccat aatccattta tgccgcggct gttccagcaa ccgcagcaac
     4981 cgccgggcgc ggcccaggtc aaggaacgca tcgcgatgat gcggcaaaag ctcgccgacg
     5041 ccaatcccga tgtccttgtt tgtatcggca acgatcactt gcatcagttt ttcatggaca
     5101 acatgccggc ttttatgatc ggcaaaatgg agcagtacaa cggcacgttt tacgacgagg
     5161 agcgtgaatt cggcttgccg cgatgcaagc ttcccggaga cttggatgtc gcggacgcga
     5221 ttatggaagg cgccttcgac cgcggcgtcg atttttctta ctccaacgag ctcaccatcg
     5281 atcactccat tgtcgttccc atgatgttcg tacgtccggc aatggatatt cccatcgtgc
     5341 cgatcttgac caattgtatc gcgccgccga tgccgagagc gcgacggtat ttcgaagtcg
     5401 gcaaagcgct tcgcgacgcc atcgacagtc tgccggcaac taagcggatc ggcgtcctgg
     5461 tcagcggtca tttgtctttg gagatcggcg gacccaaaca attcgagcgc catcttaccg
     5521 atcccaattt cgatgccgct gcggtgggct ggatcatgaa cggtgatatc gatggcgccg
     5581 cgaccggctg cacgccagaa aagatgatcg ccgcgggcaa catgactagc ggttatctaa
     5641 atttcttgat gatgatgggc ttggcaaatg ccaccaaacc gtcccacgcc gaaggcttgg
     5701 acgcgggctt cccagcgatt ccgttcttta gctgggataa acaacggtag tgtcacacgc
     5761 ccgagtcgca actaaagcaa agggagttca ccacgaagta tttcatgatc ttacgttcgc
     5821 ccttagacgt ccgctcggaa tgacaacgta tgtcatctcg aacctttcga ctgtgctagg
     5881 atgaactctg tgagaaagtt tctttcagtc ctattctcag gggaaaccgc gaagcgcatg
     5941 aagtaaacca cgtcggatta ctttgtgacc ctcatgtcgt tcgtggtgag catcccattt
     6001 cgaggagaac gcaaaaaggt catggacttt agagttcttg cgacaatctt tacttccgta
     6061 ttcatcgccg agctcggcga caaaactcag ctcgctacaa tgcttttcgc tgcagacaaa
     6121 caggtcagca agctgaccgt cttcatcggc gcgtcgctcg cgctcatcgt cacttctgcc
     6181 attggggtca ttgccgggtc ggcgatctca aactacgtca gcgaaaagac tttccattat
     6241 ctggcgggcg ccggcttcat cgcgatcggc gtctggactt tggtcaaggc ctaaggagcg
     6301 aatatgagca aatatctgat gaacaaactc atccatctga tcaacatgaa tgaggaagcg
     6361 gagaaggaat acaaggctcg accgcgagaa tttgttgagc aatgggagca aacccagacg
     6421 ctgaaactcg agcccgaaga gcgtgaagcc cttgcaacca aagactacgg caagctctat
     6481 gcgctcggcg cgcacccctt tacgctctgg agcttcaccg aagccgtgtg ggtgcacgaa
     6541 agaccgcgcg aggagctagt tgccgattac aaagccaaag ccgcccaagc cggttatcct
     6601 agtttcaaaa cttgaaaaga tc
//
LOCUS       DASULZ010000244         3941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_30431, whole genome shotgun sequence.
ACCESSION   DASULZ010000244 DASULZ010000000
VERSION     DASULZ010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30431"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..751)
                     /gene="pal"
                     /locus_tag="VFY96_04970"
     CDS             complement(179..751)
                     /gene="pal"
                     /locus_tag="VFY96_04970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEX5605844.1"
                     /translation="MGSFGKVIFGFMVLPVSIALAGCSSTNPKPEAEQAASASTPAPG
                     DKSAGAAKSSSTSSSSLEAAQRGESSATPASSPLKDVYFDFDSYDLRADARATLKANG
                     EWLRSNPSAQVQIEGHCDERGTTEYNLALGSKRAQNVKDYLVTLGAAADRLSTISYGE
                     ELPVCTEHNEACWQKNRRARFVTQTARPTS"
     gene            971..1888
                     /locus_tag="VFY96_04975"
     CDS             971..1888
                     /locus_tag="VFY96_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015143435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX5605845.1"
                     /translation="MKLRSIWHFCRRVVVQWINNEPFELAAALSYYTLFSLTPLLVIA
                     IGIAGIAFGHETAQQQILETIQDLIGPEGTEAVRLMIQNVSSRPETGKISTLIGFAVL
                     FFGAGGVVGELQSALNKVWEVTPKSSSSAIWGLLRQRFISFAMVLGIGFLLLISLIAS
                     AILSGLTELLGQFVGATAVLVHGLDIIVSFGFVTLLFALIYKFVPDARIQWRDVAIGA
                     ALTSLLFTIGKFCIGLYLGTSGVTSVYGAAGSLITILLWVYYSSLIFFFGAQFTRTYA
                     SEYGSGIIPADHAERVTVTSKPSPAAQRN"
     gene            complement(1885..3339)
                     /locus_tag="VFY96_04980"
     CDS             complement(1885..3339)
                     /locus_tag="VFY96_04980"
                     /inference="COORDINATES: protein motif:HMM:NF016089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEX5605846.1"
                     /translation="MRAPELWIAFLRVVVGAWFLKAVWTKLSLAFAWGTLPYIAVSPR
                     FVGFQPKRVAEFAAGNPVGWYKHFLEDVVLPHASLFATLQAYAEVIVGVALLVGFCVG
                     LSALLGLVLTINYGLATQWMSFGQQGFHLLLMTSMVIFLGARAGQAWGLDGVFLRATP
                     RQRRRYLTATMALVLVAIALSVSGTAWGGEHRVFATNEKSDNVTVLQAMTHKSLGTIS
                     VGQRPRGIAASRDGRRIFVANSNSNNLSVIDSKTLKVLDTLPAGVDPEGMTVDRRGLI
                     YVVNENDSALTIIDPQRREIIKRLEVGTEPETAVVSADGRWVAVSNETSNEIYFVDTE
                     TATIIGKLSVPANPRGMRFSRDARRLFVASEKANTISLIDVGTRKLIKSVPTGGERPV
                     DVVLGPDEARLYISHGASGDVRVMEAATLNFVTSIPVGPRAWWMAQTPDNKLIYVTVG
                     RANQVAAIDVETNKVAARITAGTLPWGVAVATLD"
     gene            complement(3354..>3941)
                     /locus_tag="VFY96_04985"
     CDS             complement(3354..>3941)
                     /locus_tag="VFY96_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX5605847.1"
                     /translation="GVLMFITPHPWYPSYGTGAAAIHDQQLAGLTMWIPGGAVLAFTG
                     VCLLWYWLRDIERRTVTRERLVVISKHAQRALAISVILVGAATVFEACQDAKATAVAL
                     TGGNPADGQVAIQKYGCWTCHTIPGIAGANGVIGPPLDKVASRAYVAGRPNTPQQLIE
                     WIRRPQEVRRPTPMPDMGVTDRDARDIAAYLYTLH"
BASE COUNT          840 a         1117 c         1099 g          885 t
ORIGIN      
        1 tgccaggttc gatagcaaac tgaagagaac aaatgcggga cgcgggttac aggctactcc
       61 cgtaaccccc tgtgttttga cgtgccgccg gcgttgtggc gagcagttcg cacaattcga
      121 ggacttgtga ttgtgcgccg gatgagctcg atccggactc tggacgtatg aagaaggttt
      181 agctcgtcgg gcgcgcggtc tgggtgacga aacgggcgcg gcgatttttt tgccagcaag
      241 cttcattgtg ctcggtgcat accggaagct cctcgccata gctgatcgtt gacagccgat
      301 ccgcggcggc tcctaatgtg acgagatagt ccttgacgtt ttgcgctcgt ttcgagccca
      361 gagccaggtt gtactcagtc gttccgcgct cgtcacaatg cccttcgatc tgcacctgcg
      421 ccgaaggatt tgatctaagc cattcgccat tggcctttag agtagcccga gcgtcggcgc
      481 gcagatcata actgtcgaaa tcgaaataga catccttcaa tgggctcgat gccggagtcg
      541 cgctcgattc gccgcgctgg gcggcctcaa ggctggagga acttgtcgag ctcgacttcg
      601 ctgccccggc agatttatcg cctggggccg gagtactcgc gctcgccgcc tgttccgcct
      661 caggcttcgg atttgtcgaa gagcagccag cgagagctat cgagaccgga agaaccataa
      721 aaccgaagat taccttccca aaactaccca tcactttacc ctccttgctt gatcgtcctc
      781 caaacactgt taactgttcc gaactgtctg gtttttatta tccatttttg cgtgaaaaaa
      841 attaagaggt attgatcggc gcacatcggg cagctttcaa gagcgagtgg ggaggtgaaa
      901 ttgactcatg ggtagcaatc gatgctgtaa gggctagact aaaataacag atgctcataa
      961 gcgggcggca atgaagttga gatcgatttg gcatttctgc cggcgggtgg tcgtgcagtg
     1021 gatcaacaat gaaccctttg agctggcagc tgcgctttca tattacacgt tattctcttt
     1081 gacgccactg ctcgtgatcg caatcggcat cgctggaatc gccttcggtc atgagactgc
     1141 ccagcaacaa attcttgaaa cgattcagga tctgattggt cctgaaggaa ctgaagccgt
     1201 ccgactgatg attcagaatg tcagtagccg cccagaaaca gggaaaattt ccacgttgat
     1261 cggctttgcc gttttgtttt tcggagcagg aggcgtggtc ggcgagctgc agagtgcgct
     1321 caacaaagtt tgggaagtca ctcccaagtc gtcgtcttca gcgatatggg gactcctgcg
     1381 ccaaagattt atctccttcg ctatggttct cggcatcggg tttttgctgc tgatctcgtt
     1441 gatcgcgagc gcgattctca gcggcctcac cgaactgctg gggcaattcg tcggcgcgac
     1501 ggcggtgctc gtccatgggc tcgacatcat tgtctcattt gggttcgtga ccttactttt
     1561 tgctctgatc tataaatttg tgcccgacgc gcggatccaa tggcgcgatg tcgcgatcgg
     1621 cgcagctctg acatcccttc tattcacgat cggcaagttc tgcatcggtc tttatctagg
     1681 cacgagcggg gtcacttccg tatatggcgc cgccgggtca ctcatcacga ttctgctctg
     1741 ggtttactat tcctcattga tctttttttt cggtgcccag ttcacccgaa cctatgcgag
     1801 tgaatacggc tcgggaatta ttcccgccga tcatgccgaa cgggtaacag tcacatcaaa
     1861 gccgagccca gcagctcagc gaaattagtc gagggtcgcg accgcgacgc cccacggcaa
     1921 tgttccggcc gtaatccggg ccgccacttt gttcgtttcc acatcaatgg ctgcgacttg
     1981 gttggcacgg ccaacggtga cataaattaa tttgttgtcg ggtgtttgcg ccatccacca
     2041 ggcgcgcggc cccaccggaa tcgaagtgac gaagttcaga gttgcagcct ccatcacgcg
     2101 cacatccccg gaagcgccat gcgagatata aagccgcgct tcatcggggc caagaaccac
     2161 atcgacgggt cgctccccgc cggtcggcac ggatttgatc agcttgcggg ttccgacatc
     2221 gatgagcgaa atcgtgttcg ctttctcgct ggcaacgaaa aggcgccgcg catctctact
     2281 gaagcgcatg cccctgggat ttgccggaac gctgagttta ccgataatag tcgcggtctc
     2341 ggtatcgaca aaataaattt catttgacgt ctcattggaa accgccaccc agcggccgtc
     2401 tgctgatacc acggcagtct cgggctcggt acctacctca agacgtttta tgatctcgcg
     2461 ccgctgggga tcgatgatcg tgagcgcgga atcgttctca ttgacaacgt agatcaagcc
     2521 gcgccgatcc accgtcattc cttcgggatc cacgcctgcc gggagcgtgt cgagcacttt
     2581 caaggttttg ctgtcgatga cactcaggtt attgctgttg ctgttggcaa cgaaaatacg
     2641 gcgtccgtct cggctcgccg cgatcccgcg gggccgttgg ccgacgctaa tcgtccccag
     2701 cgatttatga gtcatggcct gaaggactgt gacattgtcg gatttctcgt tagtggcgaa
     2761 gacacgatgc tcgcctcccc atgctgtgcc ggagacggag agcgcaatag caaccaggac
     2821 aagcgccatg gtagcggtta aatatctgcg tcgttggcgc ggcgtcgccc tgagaaaaac
     2881 gccatcgagg ccccacgcct gacctgcgcg agcaccgaga aaaataacca tcgaggtcat
     2941 cagtagcaga tgaaagccct gctgcccgaa actcatccat tgggtggcga gaccgtaatt
     3001 gatcgtgaga actaatccaa ggagcgcgct cagccccaca caaaaaccta ccagcagcgc
     3061 cacgccgaca atcacttcgg cgtaggcctg aagcgtggca aacaaactgg cgtgcggcag
     3121 caccacgtct tctagaaaat gcttatacca gcccaccgga ttaccggcgg cgaactcagc
     3181 gacgcgctta ggctgaaagc caacgaatct tggcgaaacg gcgatgtatg gcagcgtgcc
     3241 ccacgcgaat gcaagactga gcttcgtcca tacggctttc agaaaccaag caccgacgac
     3301 gacccgtaga aaagcaatcc agagttcagg cgctcgcatg gttcacctct caatcagtgc
     3361 aatgtataga gataggcggc gatatcgcgc gcatcgcgat cggttacacc catgtcaggc
     3421 atcggtgtcg gacgacgaac ttcttgcgga cggcgaatcc actcaattag ttgttgcgga
     3481 gtgttcggcc gacccgcgac gtacgctcga ctggcaactt tgtcgagcgg cggtccaatg
     3541 acgccgttcg cccccgcgat tcccggaatc gtgtggcagg tccagcagcc gtatttctgg
     3601 atggccactt gtccgtctgc gggattgccg ccggtgagag cgacggcagt cgccttcgca
     3661 tcctggcatg cctcgaagac cgttgcggca ccgactagaa tgacggaaat cgccagcgca
     3721 cgttgcgcgt gtttcgagat cactacgagc cgctcgcgcg taacggttcg acgttcgatg
     3781 tctcgcagcc aataccaaag caggcagacg ccggtaaatg cgagaacagc cccgcctgga
     3841 atccacatag tgagcccggc gagttgctgg tcgtggattg cggccgctcc cgttccgtaa
     3901 ctgggatacc atgggtgcgg cgttatgaac atcagtacgc c
//
LOCUS       DASULZ010000245        14343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1247229, whole genome shotgun sequence.
ACCESSION   DASULZ010000245 DASULZ010000000
VERSION     DASULZ010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 14343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14343
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247229"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            497..1264
                     /locus_tag="VFY96_04990"
     CDS             497..1264
                     /locus_tag="VFY96_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605848.1"
                     /translation="MRQRLLRKSRKILITLLVLLHSPPGTAGERPRNASVAPRSNGSE
                     TELARARTEVIQKMRETREGAEKLLALHESARQRSAVEYERRRELYYQGLIARNEVLQ
                     AEHSLAEAITRVEEDKRWLVESDMALTEITMRDELLRLPRLSVGGYSETGTLIRFNGE
                     TPWRLPDATKIEKFFLNTFGRTLPISAMGQTATHERLRFDHRNAMDVAVHPDSKEGQS
                     LLSYLRQAGIPFIAFRNAVPGAATGAHIHIGKPSPRG"
     gene            1324..2040
                     /locus_tag="VFY96_04995"
     CDS             1324..2040
                     /locus_tag="VFY96_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605849.1"
                     /translation="MPEPAIPQPASTVVLLRPNGKNGFEVYLNRRPAHLDTYAGVFVF
                     PGGRVEESDCSAAMLALTRGLTASEAQEKLGVRSPAEICLGYWVAAVRELFEEVGIHF
                     FVSDPGTSGSSLNNIQQRMSERRPLLQNGKISFAELLTAERLCCDLSRLTYFFHRVTP
                     EHYRVRFDTRFYIAALPPDQSPLAVSEEVSESLWISPQLALERFAQNEFPMMPPTVMV
                     LRTLIKYRSWTELRDLFGVC"
     gene            2308..4029
                     /gene="glgP"
                     /locus_tag="VFY96_05000"
     CDS             2308..4029
                     /gene="glgP"
                     /locus_tag="VFY96_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX5605850.1"
                     /translation="MTNPLPPTGTIAYFSMDIAVDSKIPTYSGGLGVLAGDMLRSAAD
                     LAVPMVAVSLVHRKGYFDQRLDEGGNQLESPAKWSPESQLMRVSPRVIVAVEGREVRV
                     SAWKYLFQGITGFSIPLYLLDTDLEENDPADRGLTDFLYGGDERYRLCQEVILGIGGI
                     AMLRALGYAELRVFHMNEGHSALATLALMEEQAGGLPDRSYTELEVNAVRRRCVFTTH
                     TPVPAGHDRFNADLVAQVLGKQRADALARLSVMDGALNMTELALRFSGFVNGVSLRHG
                     EISRAMFPNYTIAAITNGVHAATWASAPFAELYDRALPEWRRDNCYLRYAVDLPLAIV
                     RQTHLEAKKELLNHVRWLTGVQLDEKVFTLGFARRATAYKRGDLLFIDRERLKQIARQ
                     VGPIQLVYAGKAHPRDEGGKAIIRRIFEAAAVLANDVRVVYLENYDMALGKLLCSGVD
                     VWLNTPLRPQEASGTSGMKAALNGVPSFSVLDGWWIEGHVEGVTGWSIGDSGTENNSD
                     AEALSLYDKLAGVILPLYYKDADKFTQVMRSAIALNGSYFNTQRMIFQYVRNAYSHVG
                     AQDGQLD"
     gene            complement(4057..4572)
                     /locus_tag="VFY96_05005"
     CDS             complement(4057..4572)
                     /locus_tag="VFY96_05005"
                     /inference="COORDINATES: protein motif:HMM:NF020091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HEX5605851.1"
                     /translation="MARDLKEEFFRLDGAWASFELMSIRKRDDYLKPFRVLRCKIDDQ
                     VDFRASESLRSTTEASLLIEMFGAEELVAATGRGPVHAVDNAMRKVLEKHYPQLAEVR
                     LEDFDVRLLHGAAENDEEYGAAALVRVLGIFTDDRDRWGTVGVAADILQASIECIIDG
                     LEWKLRGEQKH"
     gene            4843..5412
                     /locus_tag="VFY96_05010"
     CDS             4843..5412
                     /locus_tag="VFY96_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605852.1"
                     /translation="MQDRHSNPRLQDFFVKIVRQSFWQLGIYDATVADYVADVLADFA
                     RTDNLYKLRGRAGRKIDSVAEMLLRQPLARNDETDVLRERSLRKYVGDYALFMSGMFR
                     KHVEGSGSLDYYIQEGSRSYWTVSELDLSLYRTGYFLYQELSKKFEYYSGALDYTRKA
                     YFAAAPGEDPFSGFVRQIEGWMKVNLTEN"
     gene            5467..6492
                     /gene="mltG"
                     /locus_tag="VFY96_05015"
     CDS             5467..6492
                     /gene="mltG"
                     /locus_tag="VFY96_05015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX5605853.1"
                     /translation="MKALRATLFATLICLVLLAGTFLYNIFYSTPGAAALPAEVRIEQ
                     GDSFATVARKLRAQNIISNAIFFSLWARVVGSDKKIHQGLYRFTDEVSPREVLERLVT
                     GKGIFQIVTIPEGLTVREIAALLEKMQIANQEKILAAASDPALLATVGLQGKGIEGYL
                     FPNTYYFVPGTPERDILLTMTEQFRKSVQPLLTQNVTEITPHEIVTLASIIEKETGVE
                     SERPLVSAVFHNRLKRQMPLQSDPTVIYGLKDFNGNLTRKDLNDPSPYNTYRVAALPP
                     GPICNPSLSSIRAALYPADAPFLYFVSKNDGTHLFSETIEAHNHAVRIYQPVRESAAR
                     SRSVDRH"
     gene            6536..7924
                     /locus_tag="VFY96_05020"
     CDS             6536..7924
                     /locus_tag="VFY96_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605854.1"
                     /translation="MQAKPHAHERSGAEILLDRLAARLNQHALWNTLLLIVPPFGAAI
                     YALAMLFGGAAVDPWTIGIIVILALAGAIIAATFYYRRCAPTIPKVAELADRQSGAKD
                     HFLTLATLPSGNYPVSFILRLRRESSAFGNRIEIKRDFPYKIQRSAYGSIGASLLAVL
                     LIYLVTPMVSAGSRVVPVQTRLRQLAQEMAKKPELKSLASQLQALAAKLEDPKATLQE
                     KQQLAQEIEKKIEEQQKQEETKDSHNLLGEAAGAVEGAQQQVASAKQSPKEQQEGGGG
                     IQSSLPEKGEGESKESQGAGAGKGDSKTESTSDLQQGNASMPKPNEPAQDKNSQQGET
                     ADRNQTDPNQATKDPNKEQAGKTQGGSKEGAGKQQASDQPPPQGVAPADRLYPSGEGK
                     QGLQGAGYVTVQLPEEIAAEGKFESRPSKDSKGGRSRSQIPASNVPLPAHIPNAASEK
                     QQLPLEYRGMIR"
     gene            7924..8925
                     /locus_tag="VFY96_05025"
     CDS             7924..8925
                     /locus_tag="VFY96_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5605855.1"
                     /translation="MEPRLMQPEQFQQTFNRLEAEVQKVIVGHNDLIRKILIAFFAGG
                     HVLLEGVPGLGKTLLVKALTHALGMSFKRIQFTPDLMPSDIVGTEVLTDTSGRREFQF
                     KKGPIFAHVVLADEINRATPKTQSAVLEAMEEKQVTVFGESHVLASPFIVLATQNPIE
                     LEGTYPLPEAQLDRFFFKLLVMPPAPPELREILKRTTGAETPDAGRVLPDDNGDLVNA
                     MKLLLRQVMIAPPIEDYVVRLVHATQPDGQNGGAARTTVKQYIRFGSSPRGAQAIILG
                     AKGNALVEGRVHVSYEDVEKITLPALRHRIILNFQAEAENITADQVIGEVMKQVPRQ"
     gene            complement(8937..9848)
                     /locus_tag="VFY96_05030"
     CDS             complement(8937..9848)
                     /locus_tag="VFY96_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEX5605856.1"
                     /translation="MKNFVNAVSSIVFSLLIVLAVAIFQPTAAAQTDNPEAMAKGQYV
                     FALAGGCACHTVPKGPLNAGGREFPIPTGAVYSTNITSDKETGLGAWTDQQILDAITK
                     GISRDGSRILPVMPYEKYSGMAQQDLKALIAYLRTLKPIKKTTPPLKSWAPLMRSVGT
                     LVYLKAFGRFSSSPAEAPSSGVERGRYLVDHVSACGDCHTPRNSLGVPNQSLYLAGAS
                     KKIGPLGNAVPNITPDKETGIGEWKGEDIVELLHSGTKPDLDNVQGLMYEVIQGAPYG
                     FKEMKKEDALAIADYLKSIPPIKNKIE"
     gene            complement(9845..10777)
                     /locus_tag="VFY96_05035"
     CDS             complement(9845..10777)
                     /locus_tag="VFY96_05035"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX5605857.1"
                     /translation="MRIAVIGTGAVGGYFGAKLAAAGHELVFLARGKHLEALQAAGLR
                     ILSPNGDLHIQKADFLGNAAQVGVVDLILFCVKSYDTQAAAESLGPLLTTNTAVVSLQ
                     NGVDNPEKIAAIWRDQRIYPGVVYIGAEIASPGVIRHSNGGKIVFGAPDGQADESAVR
                     IEQLLSQAEIPCQLSANIHEVQWSKLLWNAPFCAISCLARADVKQIVDSAPLTRLAID
                     CMAEVQSAAELRGITLPRTSFDQTIAFSRGLGAFKPSMLQDLEAGKPLEYEAFNGIVA
                     KLLQDSGKAAPINQAFHSLLQHLDKSTRESTKPT"
     gene            10881..11081
                     /locus_tag="VFY96_05040"
     CDS             10881..11081
                     /locus_tag="VFY96_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605858.1"
                     /translation="MEKIDPQGDHEALKNFEPGCSVSFRGKTYKIQRRTTLASGEAAV
                     VLQNDQEQFVISAARFLADVRT"
     gene            11118..12110
                     /locus_tag="VFY96_05045"
     CDS             11118..12110
                     /locus_tag="VFY96_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017727520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX5605859.1"
                     /translation="MAKTRLTLACWDYDRTRALQDGRVEVEGVDLTYLPLRVEETFWR
                     MLRYGEFDVAEMSTGSFLMARDKGAPRLIGIPVFPSKTFRHSCIYINTDSEIQKPADM
                     LGKRVGVPEYQITMATWARGILQHEYGVAPEKMKWFTGGEEHPGRQDKVRHNLPPNID
                     IQAIPADKTLSGMLERGEIDAMISAHMPSPFVRRHPKIRRLIPNFREVEAEYFQRTKI
                     FPIMHTIVIREEVYEKQPWIAQSLFKAFDESKRLCKEAMYEFSALKYMLAWSIDEMEK
                     EREILGDDPWAYGLEPNRHVLETLIQYTYEQGLISKSFEVESLFAKSTLEEFKI"
     gene            12122..12649
                     /locus_tag="VFY96_05050"
     CDS             12122..12649
                     /locus_tag="VFY96_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605860.1"
                     /translation="MADEKTQAFEETKWWIDRGPVKEREKPAVAPVKGIVEKWAYDLL
                     KNYNQDQAAKNLVDINSKILNGLSIGSGEWGYYSLEIGTYPVEKLNKIKSGLPLPQGA
                     RGVVVDAGFSVRVLITGEPVGARFVDINFTDAPSLDYWVNGQLVEERVFANMGEALKR
                     IRVELADYLRRPQDS"
     gene            complement(12646..12855)
                     /locus_tag="VFY96_05055"
     CDS             complement(12646..12855)
                     /locus_tag="VFY96_05055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX5605861.1"
                     /translation="MPIFEYACRRCQHRFETIVLSTNERINCPKCASVSVEKQLSVFS
                     SPGATKEAAPSSGGCACTPQTCGCH"
     gene            complement(12869..14044)
                     /gene="ribB"
                     /locus_tag="VFY96_05060"
     CDS             complement(12869..14044)
                     /gene="ribB"
                     /locus_tag="VFY96_05060"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEX5605862.1"
                     /translation="MPLASIEEAVDEIRRGGMVILMDDKDRENEGDLCMAAEKVTPEA
                     INFMATHGRGLICLPLTEERVRHLGIAMMVSDNTSPFGTAFTVSVDAATGITTGISAA
                     DRAKTILDAIADVAKPHDLVTPGHIFPLRARNGGVLVRAGQTEGSVDLARLAGLKPAG
                     VICEIMKDDGTMARQPDLMRFAKKHKLKVVTIADLIQYRLNYDLLVYKAASAPLPTRV
                     GGLFQAIVYNTLVDQSEHLALIKGEISAREETLVRVHTKYVPGDVFGFELLNTNAVIQ
                     RSMEVIAKEGRGVILYLQPQRKGLRPAMVAYPKLEGKQQKEMNRSFVYQADFKEYGIG
                     AQILRDLGVRKMRLLSNNKKHLVGLRGYGLEITALEPIRDNVVRETAKPKKTKLRHA"
BASE COUNT         3257 a         3819 c         3988 g         3279 t
ORIGIN      
        1 aggaagcacc gcggattgaa cgatattttg cccagaattt tgggcgacag ttgcccgtaa
       61 ccgctttggg ccaatccgag acgcacaatc ggctgggttt cgatcaccac gacgcgatgg
      121 atattgccgt ccacccagac agcgcggaag gtaaagcgct catccaacaa ctgcgcaaaa
      181 tgagcgtccc ctttatcgcg ttccgaggcg cggttcctgg ggcttccaca gggcctcaca
      241 tccacgtcgg tcgcccatcg ggtcgggtca cgcgcggctg acgccgatgc accggacact
      301 ggtgccgtat tcggccggtt ggaaccgtta atcaaacaca gataaagcct tctaccttac
      361 actccctgag tgtcccggca gttggccata cgcaagagga cgtgcagaga gccggtgcag
      421 agaagccgag cccatactcc gacgcccgcc cttgacccat aaacgcttac tacctaaact
      481 cggacacgat caaagaatga gacagcgttt gttgcgcaaa tctagaaaaa tactgattac
      541 cttgcttgtg ctgttgcact cacctcctgg aacggccggt gagcgtccac gcaatgcctc
      601 cgtagctccg cgcagcaacg gaagcgaaac ggagttggcg cgcgcgcgta ccgaggttat
      661 tcagaagatg cgggaaaccc gcgaaggagc cgagaagcta ctagcgttac atgaaagcgc
      721 ccggcaacga tcggcggtgg aatatgagcg gcggcgcgaa ctctattacc aagggttgat
      781 cgcacgcaac gaggtgctgc aagcagaaca ctcgcttgcc gaggcgatca cgcgtgtgga
      841 ggaggataag cgctggttgg tggaaagcga tatggcgctc acggaaatta ctatgcgcga
      901 tgagctgctt aggttgccgc gcctttcggt gggcggttac agcgagaccg gcacactgat
      961 tcgttttaat ggcgagacgc cgtggcggct tccagatgcg acgaaaatcg aaaagttttt
     1021 cctgaacacc ttcggtcgca ccttgccgat tagcgccatg ggtcagaccg cgacgcacga
     1081 gcgtctgcgc tttgatcacc gcaacgccat ggatgtggcg gttcatccgg acagcaaaga
     1141 agggcaatcg ttgctgagct atctgcggca agcaggtatt cctttcatcg cgttcagaaa
     1201 tgctgtgcca ggcgcggcaa ccggcgctca cattcatatc ggcaagccat cgccgcgggg
     1261 gtgaaaaaat ataagcaata tgatcgcgac gttaacagca gttcggatga gagctttact
     1321 ctaatgcccg agcctgccat tcctcagccc gcgtcgacag tggtgctttt acggccgaac
     1381 ggaaagaacg gcttcgaggt ctatttgaat cggaggccgg ctcacttgga tacgtatgcc
     1441 ggtgttttcg tttttccggg ggggcgcgtt gaagagagcg attgctccgc agcgatgctg
     1501 gccctcacgc gtggccttac tgcatccgaa gcgcaggaaa agctcggcgt ccgctcgcca
     1561 gcagaaatct gcctgggtta ctgggtcgcg gctgtaagag aactcttcga agaagtggga
     1621 attcactttt tcgtttccga cccggggacc tctggttcat cgttgaacaa tattcagcaa
     1681 cgtatgtctg agcgcaggcc gctgttgcaa aacgggaaaa ttagctttgc cgagctactg
     1741 acagccgagc ggctgtgttg cgacttgagc cgtctaactt attttttcca tcgtgtgacc
     1801 cctgaacact atcgggtgag atttgataca cgattttaca tcgcggctct tccgcccgat
     1861 caaagtccgc tggctgtctc tgaggaagtt tctgagagcc tgtggatttc gcctcaactc
     1921 gcgctcgaac gttttgcgca gaatgaattt cccatgatgc cgccgaccgt gatggtgttg
     1981 cgcacgttga ttaagtatcg gtcctggaca gaattgcgcg acctgtttgg cgtttgttag
     2041 gcaggcggca agaacccggt tcaccgcgaa actatttttt cataaagtct tcgcttttcg
     2101 tttcccttac ccgtcaggcc acatttcagg tgcattttgg tgttaaatgg cactatcctt
     2161 gctttgtcac agcgaattgc ccgcgtgagg tccttatgtt atggctagtt taacttcgac
     2221 actccggaga gactcggtac attgtcgcat cctctcttat ccactcgatc gattgcttcg
     2281 taatttcgtc aaatagagca acgggctatg accaatccat tgccccccac ggggaccatc
     2341 gcctattttt ccatggacat agcggtggat tcaaaaatcc cgacttacag cggcggtttg
     2401 ggcgttttgg ccggtgatat gttgcgttcc gccgctgatc tggcggttcc aatggttgcc
     2461 gtgtctttgg ttcatcgtaa aggttatttc gatcagcgcc tggatgaagg cggaaatcag
     2521 ctggagagcc cggccaaatg gtccccggaa agccagctga tgcgtgtttc acccagagtt
     2581 atcgtggccg tggaaggacg tgaagtccgc gtgagtgcct ggaagtattt gtttcagggt
     2641 atcaccggat tctcgatccc gctttatctt ctcgatacgg atctggagga aaacgatcct
     2701 gcagatcgcg gccttacgga ttttctctat ggcggcgatg agcgctatcg attgtgtcag
     2761 gaagtgattt tggggattgg cggcatcgct atgctgcgcg ctctcggcta tgccgagctt
     2821 cgtgtttttc acatgaacga aggccattcg gcgttggcga cgcttgcgtt gatggaggaa
     2881 caagcgggcg gcttgccgga tcgaagttac accgaattgg aagtgaacgc tgtacgacgc
     2941 cgatgcgtat ttactaccca tacaccggtc cccgcaggtc atgaccggtt caacgccgat
     3001 ctcgttgctc aagttttggg caaacagcgt gcggatgcgc tggcacgtct ctcggttatg
     3061 gatggcgctc tcaacatgac ggaactagcc ctcaggttct ctgggttcgt caacggcgta
     3121 tctttgcggc atggcgaaat ttcccgcgcg atgtttccaa actatacgat cgcggcaatc
     3181 accaacggcg tccacgcagc gacttgggcg agcgctccgt tcgcggagct atacgatcgt
     3241 gcattaccgg aatggcgccg ggacaattgc tatttgcgct atgcggtcga tctaccgctt
     3301 gcgatagttc gacaaaccca cttggaggca aaaaaggaac tgctcaacca cgttcgatgg
     3361 ctcaccggag ttcagctcga cgagaaggtc tttacattgg gttttgcgcg ccgggcgact
     3421 gcgtacaaga gaggcgatct gctttttatc gatcgggagc gtctgaagca gattgcgcgt
     3481 caagtcggcc cgatacagct cgtctatgcc ggaaaagcgc atccacggga cgagggtggc
     3541 aaggcgatta ttcgccggat tttcgaggcc gcggcggttc tcgccaacga tgtgcgcgtg
     3601 gtttatctgg aaaactacga catggctctc ggaaagctgc tttgttccgg cgtcgacgtt
     3661 tggctcaaca cgcctcttcg cccgcaggaa gcgtcaggaa cgagcggcat gaaagcagcg
     3721 ttgaacggtg tgccgagctt cagcgttctg gatggttggt ggatcgaggg tcatgtggaa
     3781 ggtgtcaccg ggtggtcgat cggtgatagc ggtacggaga acaattccga tgcggaggcg
     3841 ttatcgctgt atgataaatt agctggagtt atcttgccgc tttactacaa ggacgcagat
     3901 aagttcacgc aagtcatgcg ctcggccatc gcgctgaatg gctcgtattt caacacccaa
     3961 cggatgatct tccagtatgt gaggaatgct tatagccatg tcggcgctca ggacggccaa
     4021 ttggattgag gttggctctg gaccaataga aaccgttcag tgcttctgct cgccacggag
     4081 tttccactct aacccgtcaa taatgcattc gatgctggcc tgaagaatat cggcggccac
     4141 tcccactgtt ccccaacgat ctcgatcatc ggtgaaaatc cctaaaaccc gcaccagtgc
     4201 ggctgcgccg tattcttcgt cgttttcggc cgcaccatgc agcagccgga cgtcgaaatc
     4261 ttccaaccga acctcggcga gttgcgggta atgtttctcg agaactttcc gcatggcgtt
     4321 gtcgacggca tgcaccgggc ctcgtccagt ggcggcgacg agttcttcgg cgccgaacat
     4381 ctcaatcaat aggctggctt cggtcgtact ccgtaacgac tcgctcgcgc gaaaatcgac
     4441 ctgatcgtca atcttacaac ggagtacgcg gaatggtttc agataatcat cacgtttacg
     4501 aatcgacata agctcgaagg aagcccatgc gccgtcgagt cggaaaaact cttcctttaa
     4561 atctcttgcc attcccttct cccttcacaa ctatgacgga actgccattg atagccgaag
     4621 acataccgct tcggtttgca aactggctga gcgagtacca ccctgccggc catgacgatc
     4681 tattttttca attccgcagg cctgtaagcc cgcgatgctc ggcgctggaa cggtccttct
     4741 attatagcga gccggcttca cttcgctact tgagcagcgg cggtgctcca atgaaagtac
     4801 cttttcacat tatggcaact ttgctatcat tcttttgtgg cgatgcaaga ccgacattcc
     4861 aacccccgcc tgcaggattt ctttgttaag attgtgcgcc agagtttttg gcagttaggg
     4921 atttacgatg ccactgtggc ggactatgtc gcggacgtcc ttgccgattt tgctcgtaca
     4981 gataatcttt acaaacttcg cggacgggcg ggtcgaaaga tcgatagcgt agcggaaatg
     5041 ctgctccggc agccgctcgc gcgtaacgat gagaccgatg ttctacgcga gcgctcgtta
     5101 cgtaaatatg tcggtgatta tgcgctattc atgagcggca tgtttcgaaa gcacgtagaa
     5161 ggcagcgggt ctctcgatta ttacattcaa gaaggcagcc gttcctactg gacggtttcc
     5221 gagctcgatc tctcgctcta ccgtacaggt tattttctat accaagagct ctcaaaaaaa
     5281 tttgagtact attccggcgc gctcgactat acgcgcaagg cctactttgc ggcggctcct
     5341 ggagaggatc cgttttccgg gtttgtgcgc caaatcgaag ggtggatgaa ggtcaatttg
     5401 accgagaatt agtcggcaat tgatctaact aacgcgtcca ttttcgttcc gccagcctga
     5461 ctcatcgtga aagctttacg ggctacacta ttcgcgacat tgatttgcct tgtcctttta
     5521 gcggggacgt ttctttacaa tattttctat tctactcccg gggcggcagc tttaccggcc
     5581 gaggtgcgga ttgaacaggg cgattctttc gccacggttg cgcgcaaact gcgcgcgcaa
     5641 aacattattt caaacgcaat ctttttttcg ctttgggccc gtgttgtcgg ctcggacaaa
     5701 aagatccatc agggtctcta tcgctttacc gacgaagtct cgccgcggga agttttggag
     5761 cggctggtca cgggtaaggg catctttcaa attgtgacga tacctgaggg tctcacggtc
     5821 agagaaattg ccgcgttgct ggaaaaaatg cagattgcca accaagagaa gatactcgca
     5881 gcagccagtg acccggcgct gctggcaact gtcggcttac agggcaaagg aatagaaggc
     5941 tatctgtttc cgaataccta ttatttcgtt ccgggcacgc ccgagcgaga tattctgctc
     6001 accatgaccg agcagtttcg aaagtccgtg caaccgcttt tgacccaaaa cgtcacggaa
     6061 attacgcccc atgaaatcgt gacgctcgcc tcgattatag aaaaagagac cggagttgaa
     6121 tcggagcgcc cgctcgtctc cgcggttttt cacaatcgac taaaacgcca aatgccgctg
     6181 caaagcgacc ccacggtcat ttacggcctg aaggacttca atggcaattt aactcgcaag
     6241 gatctcaacg atccgagtcc gtacaatacg tacagagttg ccgcgctgcc ccccggaccc
     6301 atttgcaatc caagcttgtc ttcgattcgc gccgcgttgt atccggcgga tgctccgttt
     6361 ctttattttg tctccaaaaa cgacggtact catttgttct ctgagaccat cgaagcgcac
     6421 aatcacgccg tgcgaattta ccaaccggtt cgtgagtcag cggcgcgctc gaggtccgtc
     6481 gaccgccact aggctgcgct taagccattc gcaccggcat gctatagtct tgccaatgca
     6541 ggcaaagcct catgcgcacg agcgtagtgg agcggaaatt ctcctcgatc gtttagctgc
     6601 gaggttaaac cagcacgcgc tctggaatac tctgcttttg attgtgccgc cgtttggcgc
     6661 ggcgatttat gcacttgcaa tgttgttcgg cggggccgcg gtcgatccat ggaccatcgg
     6721 gatcatcgta attctggcgc tcgcaggagc aatcattgcg gcaacgtttt actatcgccg
     6781 atgtgccccg actatcccca aggtggctga actggccgac cggcagtccg gcgccaaaga
     6841 tcatttttta actttagcga cgctcccatc ggggaattat ccggtctcat tcatattgcg
     6901 gctgcgccgg gagagtagcg cattcggtaa ccggatcgaa atcaaacggg attttcctta
     6961 taaaattcaa cgctcggcct atggctctat aggcgcttcg ctgttggcag ttctcttgat
     7021 ttatctcgtc acaccaatgg ttagcgcagg gagtagagtc gtgccggtcc aaactcgact
     7081 tcgtcagctc gctcaggaga tggcgaaaaa accggagctc aaatcgctgg cgagtcaact
     7141 ccaagcgctt gcggcaaagc tcgaagatcc aaaggcgacc ctacaagaaa agcagcaact
     7201 ggcgcaggaa atcgaaaaga aaatcgagga gcagcaaaag caagaagaaa cgaaagacag
     7261 tcataattta ctcggcgaag cggccggtgc tgtggaagga gcacaacaac aagtcgcaag
     7321 cgccaagcag tcgccgaagg aacagcagga gggtggcggg gggattcaga gtagtttgcc
     7381 cgagaaggga gagggagaaa gtaaagaaag ccaaggcgcc ggggcaggta agggcgattc
     7441 caaaacagaa tccacttccg atttgcagca aggaaatgcg agcatgccaa aaccaaacga
     7501 gcccgctcag gacaagaatt cgcagcaagg cgagacagcg gatcgtaacc agacagatcc
     7561 gaaccaggcg actaaagatc ccaacaagga acaagcagga aaaacccagg gcggatccaa
     7621 agaaggagcg ggcaaacaac aggcgtcgga tcaaccaccg ccgcaaggcg tggcgccggc
     7681 ggaccggcta tacccgtcag gtgagggcaa acaaggactt cagggcgcag gctatgtcac
     7741 cgtgcagttg cctgaagaga ttgcggcgga gggaaaattc gagagccgcc caagtaagga
     7801 ttcgaaaggc ggccggagtc ggtcgcaaat tccagcgagc aatgtgccgc taccggctca
     7861 cattcccaat gctgccagcg agaagcaaca actcccgctc gaatatcggg gcatgattcg
     7921 ctgatggaac cgcgcttgat gcaaccggag cagtttcaac agacgttcaa ccgccttgag
     7981 gcggaagtgc aaaaggtcat cgtcggacac aacgatttga ttcgcaaaat tttgattgcg
     8041 tttttcgccg gcggacacgt gctgttggaa ggtgtgcccg gtttgggcaa aaccttgctg
     8101 gtcaaggcgc tgactcatgc gcttggaatg tcgttcaagc gcattcagtt tacgccggac
     8161 ttgatgccgt ccgacattgt cgggaccgaa gtcttgaccg acacgagcgg acggcgtgaa
     8221 tttcagttta aaaagggtcc cattttcgct cacgtcgtcc tggcggatga gatcaatcga
     8281 gccacaccga agacgcaatc ggcggtgctc gaagcgatgg aagaaaaaca ggttaccgtg
     8341 ttcggcgaat cgcacgttct ggcgtctcca ttcattgtgc tcgcaaccca aaatccgatc
     8401 gagctcgagg gcacctaccc attgcctgag gcgcagctcg atcgattctt tttcaagctg
     8461 ctagtgatgc cgcccgcgcc gcccgagttg cgcgagattc tcaagcgcac aacgggagcg
     8521 gaaactcctg atgcgggtcg agttttgccg gatgataacg gcgatctcgt aaacgcgatg
     8581 aaactactct tgcgtcaggt gatgatcgcc ccgcccatcg aagactacgt cgtgcgactg
     8641 gtgcatgcca cacagccgga tggacaaaac ggcggagcgg cgaggacgac ggtcaagcaa
     8701 tatattcgct tcggctccag cccgcgcggt gcgcaagcga ttatccttgg cgccaaagga
     8761 aatgctttgg tcgaaggacg cgtgcacgtg agttacgagg atgtcgaaaa aatcacgctt
     8821 ccggcgctac gccacagaat tattctcaat ttccaagccg aagcggagaa catcacggcc
     8881 gatcaggtga tcggcgaagt gatgaagcag gtccccaggc agtaacttcc ccacagtcac
     8941 tcaatcttgt ttttgatcgg tgggatgctt ttgaggtaat cggcaatggc gagggcgtcc
     9001 tcttttttca tttccttaaa gccgtagggc gcgccttgaa tgacttcgta catgaggcct
     9061 tgcacgttat cgagatcggg tttggtgccc gagtgcagca gctcgacaat atcttccccc
     9121 ttccattcgc caatcccagt ttctttatcg ggtgtaatat tcgggacggc gttaccgagc
     9181 ggtccgattt tttttgacgc gcctgcaaga tacaaagatt ggtttggcac accgagcgaa
     9241 tttctcggcg tgtggcagtc accgcaagcc gagacgtgat cgaccaggta gcggccgcgc
     9301 tccacgccgc tgctcggagc ttctgccggc gagctactga aacggccgaa agctttgagg
     9361 tagacaaggg tgccgacgct tcgcatcagc ggcgcccaac ttttcagagg cggcgtcgtt
     9421 tttttgatcg gcttcagcgt gcgcaaataa gcgatcagtg ccttcaagtc ctgctgcgcc
     9481 atgccggaat acttctcata cggcattacc ggcaaaatcc ggctgccgtc gcgactgata
     9541 cctttggtaa tggcgtccaa gatctgttga tccgtccaag cgcctagccc ggtttccttg
     9601 tccgaggtga tgttggtgct gtaaacggcg ccggtgggaa tcggaaattc gcggccgcct
     9661 gcgttgagcg gtcccttcgg aacagtgtga caagcacaac cgcccgccaa agcaaaaaca
     9721 tattggcctt tggccatggc ttcggggttg tccgtttgcg ctgctgcggt gggttgaaat
     9781 atggcaacgg ccaggacgat cagcagcgaa aacacgatcg agctaacggc attgacgaaa
     9841 tttttcatgt cggctttgtg ctctcccgcg ttgacttgtc gagatgctga agcagagaat
     9901 gaaaggcttg attgatcggc gcggccttcc cggagtcttg cagaagcttc gcgacgattc
     9961 cgttgaatgc ttcatactcc aacggcttac cggcttccaa atcttgcaac atggaaggct
    10021 tgaaagcacc caggccgcgg gaaaatgcga tggtctgatc aaacgacgtt cgaggcaagg
    10081 ttatgccgcg gagttccgct gcgctctgga cttcggccat gcaatcgatc gccaggcgcg
    10141 ttagcggcgc ggaatcgaca atttgtttga cgtctgcgcg ggccaagcag ctgatggcgc
    10201 aaaaaggagc attccaaagg agcttgctcc attgcacctc gtgaatattg gcgctgagtt
    10261 gacatgggat ttcggcttgc gagagcagct gctcgatgcg gactgccgat tcatccgctt
    10321 ggccatcagg ggcaccgaag acaatttttc cgccgttcga atggcgaatc actcccggag
    10381 aggcaatttc ggcgccgata taaaccactc cgggataaat tcgctggtcg cgccagatgg
    10441 ccgcgatttt ctccgggtta tcgacgccgt tttgtaacga aacaaccgcg gtgttggttg
    10501 tgagcagtgg tccgagcgat tcagccgccg cttgggtgtc gtaagacttg acgcaaaaca
    10561 aaatcagatc aacgactccg acttgtgcgg cgttaccgag aaagtccgct ttctgaatat
    10621 gcagatcgcc gttcgggctc agaatccgca gccctgcagc ctgcaacgcc tcgagatgtt
    10681 taccccgggc gaggaacacg agctcatgtc ccgctgccgc gagcttggcg ccgaagtaac
    10741 cacccacggc gcccgtccct attaccgcga tacgcatgat gtcgaagttg ctatcgcgct
    10801 gactctaatc gcgggtcgcg ctgaagtcaa tttaacccta ggtttaagat ttactgggca
    10861 atatggtatg aattcacccg atggaaaaaa tcgatccgca aggcgatcac gaggcgttaa
    10921 aaaactttga gcccggttgt tcggtttcct ttcgcggcaa gacttacaag atccaacggc
    10981 gaacaacgct tgcatcgggc gaggccgcag tggtgttgca aaacgaccag gaacaattcg
    11041 tcatcagcgc ggcacgattt cttgccgacg tgcgaaccta aacgaagcaa ttcgcatcac
    11101 atgactagga gcagatcatg gcaaagaccc ggttgacctt ggcttgttgg gattacgacc
    11161 gtacgcgggc tttgcaggat ggtcgcgttg aagttgaggg cgtggatctc acgtatttgc
    11221 cgctccgcgt cgaggagacc ttttggcgca tgctgcgcta cggcgaattc gatgtggcag
    11281 agatgtccac gggctcgttt ctgatggcgc gcgacaaggg cgcaccgcgg cttatcggga
    11341 ttccagtctt tccttcgaag acttttcgcc actcctgcat ttacatcaat acggattccg
    11401 agattcagaa accggcagat atgttgggaa aacgcgttgg ggttcccgag tatcagatca
    11461 ccatggccac atgggcgcgc ggtatcctgc aacatgaata cggcgtggca ccggaaaaaa
    11521 tgaaatggtt caccggcggt gaagagcatc ccggacgtca ggacaaggtc agacataatc
    11581 tgccaccgaa tatcgacatt caagcgattc cagccgacaa gacgctttcg ggtatgctcg
    11641 aacgcggcga gatcgatgcg atgatttcag cgcatatgcc gtcgccgttc gtgcggcgcc
    11701 atccgaagat ccggcggttg attcccaatt ttcgcgaggt cgaagcggaa tattttcaga
    11761 ggaccaaaat ctttccgatc atgcacacca tcgtcatacg ggaagaggtt tacgaaaagc
    11821 agccttggat cgcacagagc cttttcaagg cattcgatga atccaagcgg ctctgcaagg
    11881 aggcgatgta cgagttctct gctctcaagt acatgctggc ctggtccatc gatgagatgg
    11941 agaaggaaag agaaattctt ggagacgatc cgtgggcgta tggcctggaa cccaatcgtc
    12001 acgtactgga aactctgatc cagtatacct acgagcaggg gctgatcagt aaaagtttcg
    12061 aggtcgaatc cctgtttgcg aaaagcactc tcgaggaatt taagatctga gttgcgattt
    12121 catggctgac gaaaaaaccc aagccttcga agaaacgaag tggtggattg atcgcggtcc
    12181 ggttaaagaa cgcgaaaagc cggcagtggc acccgtcaag ggaatcgtcg aaaaatgggc
    12241 ctacgatctg ctgaagaatt acaaccagga ccaagcggcg aaaaatctcg ttgacatcaa
    12301 cagtaaaatc ttgaacggcc tttcgattgg ctccggtgag tggggatact actctttgga
    12361 aatcggcact tatccggtag aaaaattaaa caagataaaa agcggtctgc cattgcccca
    12421 aggcgctcgc ggagtggtcg tggacgcagg gttttcggtc agagtgctca tcaccggcga
    12481 gccggtcggt gcgcgtttcg tcgatatcaa ttttaccgac gcgccgtcgt tggactattg
    12541 ggttaacgga caactcgtgg aagaacgagt ctttgctaat atgggcgaag cgctgaaacg
    12601 cattcgcgtt gagttggccg attatctccg aaggccgcaa gactcctagt gacagccgca
    12661 cgtttgcggt gtgcatgcgc agccgccgct cgacggagct gcttctttgg ttgcgccggg
    12721 cgagctaaac acagacaact gcttttccac agatacgcta gcgcacttcg ggcagttgat
    12781 tctttcattc gtcgaaagca cgatggtttc gaaccggtgc tgacaacgcc ggcaggcgta
    12841 ctcaaaaatt ggcatgaagt aatcctcctc aggcgtgccg cagtttggtt tttttcggtt
    12901 tcgcagtctc acgcacgacg ttgtcccgga tcggttccag ggcggtgatt tccaaaccgt
    12961 aaccgcgcag gcccaccagg tgttttttgt tgttgctcag taggcgcatt ttgcgcacac
    13021 ccagatcccg taaaatttgc gcgccgatac cgtattcctt gaaatcggcc tgataaacaa
    13081 acgagcggtt catttccttc tgctgtttgc cttcgagttt gggataagcc accatcgccg
    13141 ggcgcaaacc tttgcgctgc ggttgaagat aaaggatcac accgcgtcct tccttggcga
    13201 tgacctccat cgagcgttgg atgaccgcat tggtattgag caactcaaag ccaaaaacgt
    13261 cccccgggac atatttggtg tgaactcgca ccaaagtttc ctcgcgcgcg gagatctcac
    13321 ctttgatcag ggcgagatgt tcgctttgat cgactagagt gttatagacg atcgcctgga
    13381 agagacctcc gacccgagtc ggaagcggcg ccgatgcagc tttgtacacc agtagatcat
    13441 aattcagccg gtactggatc agatcagcaa tcgtcaccac cttcagttta tgcttcttgg
    13501 cgaagcgcat caggtcgggt tggcgcgcca tagttccgtc gtctttcatg atctcgcaaa
    13561 tcactccggc cggtttgagt cccgccaaac gggccaaatc gactgaacct tcggtttgcc
    13621 cggcgcggac cagcacgccg ccattgcgcg ctcgaagcgg aaagatatgg ccgggcgtga
    13681 ccaaatcgtg gggcttggcg acatcggcga tcgcgtcgag aatggttttg gcgcgatcgg
    13741 cggcggaaat cccggtggta attcccgttg cggcatcgac ggaaactgta aatgcagtac
    13801 cgaacggcga ggtgttatca gaaaccatca tcgctatacc gagatgtctc actctttctt
    13861 ccgtgagtgg aaggcaaatc aacccgcgcc cgtgagtcgc catgaagttg atcgcttcgg
    13921 gagtgacttt ctcagccgcc atgcagagat cgccttcgtt ctcgcggtcc ttgtcatcca
    13981 tgagaatgac cattccgcca cgtcggattt cgtcgacggc ttcttcaatg ctggctagag
    14041 gcacggggaa tcaatgtacc atcttggtgc gaaccgtcaa ccgcactttc gtcgcttacc
    14101 cgtcgaggaa accattaaaa agctggatta ttgtcatatc cgtcgtattg actttgcgca
    14161 cgtcggtgtt agcgtgtgta acatcttatg agcacacgtc tgcggaagac tcttgcggtt
    14221 gtgagcttac tcttcgtcgt cacactcagc ggcgttgcgc tcagtctggc caacgatacc
    14281 ctctcccccc aaagagatat ctttcttagc ggagtcaatt tttcgcatgt gctggcacac
    14341 cca
//
LOCUS       DASULZ010000246         2336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1146634, whole genome shotgun sequence.
ACCESSION   DASULZ010000246 DASULZ010000000
VERSION     DASULZ010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146634"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VFY96_05065"
     CDS             complement(<1..407)
                     /locus_tag="VFY96_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605863.1"
                     /translation="MALNSLEQQALAELDLPTRRPFRLADHLGAVRAASVVFLIALWE
                     IFGRQVNPLFLSYPSAIGRAFLQIVTTAEFQRSALGSLHVFAIGLLAALALGITVGLL
                     MGRYRLVEYLLDPYVYALDATPRVALIPLLLLW"
     gene            complement(658..1485)
                     /locus_tag="VFY96_05070"
     CDS             complement(658..1485)
                     /locus_tag="VFY96_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5605864.1"
                     /translation="MAVATDEQIIEQLQLRRERRWSIYDHPNLIRSASVVVFLLLWEF
                     FGRETDPILFTYPTAVAEAFVKLIVSGELIKQLAVSLSALAVGFIASLVLGVALGLAL
                     GMSRTVEAFFEPPINALYATPQVALTPLLMMWLGLGFSVKVAVVFLFAFFPILINTAS
                     GAKNVSAAMVEVARAYLAPPRQILFKVICPAALPFIMAGVRIAIGRALVGMIVAELFT
                     AVTGLGAMLSLYGNIFETAKMFVVIVVLAGLGVGLVRLTQWLERRLAIWKETERALG"
     gene            complement(1488..2249)
                     /locus_tag="VFY96_05075"
     CDS             complement(1488..2249)
                     /locus_tag="VFY96_05075"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018154474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5605865.1"
                     /translation="MLEVRRLSKIFFEQNDPRKPGLVALYNIFLAIRKNEFVSLLGPS
                     GCGKTTLIRIIAGLLTADRGEVLVNSQVVNSPGRDRCMVFQQFGLLPWRTVLSNVEFG
                     LEIDGVPRDERRALAEQYLELVGLKGFENYYPHQVSGGMQQRVGIARALSKKPEILLM
                     DEPFGAVDAQTREQLQEELLKIWAQTETTVVFVTHSIDEAIYLSDRVVVMQARPGRIK
                     EEVIIDLPRPRWEGDIKADPRFAQHRARLRDSLRS"
BASE COUNT          550 a          707 c          639 g          440 t
ORIGIN      
        1 aaccaaagga gcagtagcgg gatcaatgcc acgcgcggcg tggcgtcgag cgcgtaaaca
       61 tatggatcga gcaaatactc gaccagacgg taacgcccca tcagtaatcc gacggtgatg
      121 cccagagcca gagccgcgag cagcccaatc gcgaacacat gcaagctgcc gagcgcgctg
      181 cgctgaaatt ccgcggtggt gacaatctgt aagaacgccc ggccgatggc actcggataa
      241 gaaaggaaca gcggattcac ttgacggccg aagatttccc agagcgcaat gaggaagaca
      301 accgatgcgg cgcgtactgc gccgaggtga tcggccaggc gaaatgggcg cctcgtcggc
      361 agatccaatt ccgcgagtgc ctgctgctct aaactattaa gcgccattaa cctacgaaac
      421 caaccgatcc acagttttaa gccgagaggt tctttcgatt gtcactgcaa gtcgagttgc
      481 gtctccggca cacctcggga tccaattccc tcccgcatcc cgttaacctg aataccgtgt
      541 tccaacggca tgacggactc tgtccgtcac ctggatagcg tgggagtcga tcctgagtcc
      601 tctcggggca agcgacgtga cgaagttttg ctgcgcggaa aaaaaacttt gccatgttta
      661 tcccagcgct ctttcggttt ccttccagat cgcgagcctg cgttcgagcc attgagtgag
      721 tcgcaccagg ccgacgccca atcccgccag tacgacgatg accacaaaca tttttgccgt
      781 ttcaaaaata ttgccgtaga gcgacaacat cgcgccgaga cccgtgactg ctgtgaaaag
      841 ctcggcgacg atcataccca ctagcgcacg accgatggcg atccgcacgc cggccatgat
      901 gaacggcagc gccgcggggc agatcacttt gaaaagaatt tgccgcggcg gcgccagata
      961 ggcgcgcgcc acctcgacca tcgcggcgct gacatttttc gcgccactgg cggtgttgat
     1021 gaggatcgga aaaaaggcaa agaggaatac caccgcaact tttacgctga atccgaggcc
     1081 cagccacatc atgagcagcg gggtcagcgc aacctgcggt gttgcataga gcgcattgat
     1141 gggcggttcg aaaaacgctt caaccgtccg cgacatgccc aacgccagtc cgagagcgac
     1201 accgaggacg agtgaggcaa taaacccaac cgcgagcgca ctcaggctga ccgccagttg
     1261 cttgatcagc tcgccggaga cgatgagctt tacaaaagct tcggcaacgg ccgtcggata
     1321 ggtaaacaga atcggatcgg tttcccggcc gaaaaactcc cacagcagca aaaacactac
     1381 caccgatgcc gaacgaatga gattgggatg atcatagatc gaccaacgcc gctcgcgccg
     1441 gagctgaagc tgttcgatga tctgttcgtc ggtggcaact gccatgatca actccgcagc
     1501 gaatcgcgca aacgcgcgcg gtgctgcgcg aagcgcggat cggctttgat gtcgccttcc
     1561 caacgcggtc gcggcaaatc gatgatcact tcttccttga tgcgcccagg ccgagcttgc
     1621 atcaccacga cgcggtcaga aagatagatt gcctcatcga tgctgtgagt cacaaatacc
     1681 actgtcgttt ccgtctgcgc ccagatcttc agcagctctt cctgcaattg ttcccgtgtc
     1741 tgcgcatcga cggcgccgaa gggctcgtcc atcagcaaga tctcgggttt tttcgagagc
     1801 gcccgcgcga tgccgacgcg ctgctgcatg ccgccggaaa cttgatgggg ataatagttc
     1861 tcaaaacctt tgagaccgac caattctaga tactgctcgg ccagtgcgcg tctttcgtcg
     1921 cgcggcacac cgtcgatctc cagtccgaac tcgacattgc tcagtacagt gcgccatggc
     1981 aacaacccga actgttgaaa caccatgcag cggtcgcggc ccggcgagtt tacgacttgc
     2041 gaattgacca gcacttcacc gcggtccgcc gtcaacagtc cggcaatgat ccgaatcaag
     2101 gtggttttgc cgcagccgct cggcccgagc agcgacacga actcattctt tcgaatggcg
     2161 aggaaaatat tatatagcgc gaccaggccg ggcttgcgcg gatcgttctg ctcaaagaaa
     2221 attttggaga ggcgccgcac ctccagcatt catcaaccta ccgacgggtc gctactcgca
     2281 atccggaagc gcttattttc ggccgcccag ttccttgagc gcgcggtcaa cgaacc
//
LOCUS       DASULZ010000247         7524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1044050, whole genome shotgun sequence.
ACCESSION   DASULZ010000247 DASULZ010000000
VERSION     DASULZ010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7524
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044050"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="VFY96_05080"
     CDS             complement(<1..780)
                     /locus_tag="VFY96_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEX5605866.1"
                     /translation="MKKRLLIVLVILLVAAAGVIWWIVERHGAARELVLYGNIDLRQV
                     QLAFNNSERIAAVLVQEGDHVRKGQLLARLNTSRLEPQVAQAAAQSAAQLQVVTRMRN
                     GSRPEEIAQARANVESAKADLVNAQRQFERYRNAAEASGGRAVRQQDVDAAKAALDVA
                     VAKLAVNQKSLDLAVLGPRKEDIAENEARLHATQAQLTLLRQQLADSELIAPADAVVR
                     TRIMEPGEMATPQSPVFSLAIIDPKWVRAYVSEPDLGKIRPG"
     gene            complement(800..1390)
                     /locus_tag="VFY96_05085"
     CDS             complement(800..1390)
                     /locus_tag="VFY96_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5605867.1"
                     /translation="MAARAHFFAHGFRSVTMDDLAAELGMSKKTLYAQFPSKTELLRA
                     VLLAKFRSVETDLEAVITCSANDASAALHQLLACMQRHTEEIQPPFVRDIRRETPELF
                     QLVEERRRDLIQRYFGKIFDQGRRAGIIRKEISSELIVEILLGAVQAVMNPAKIEQLG
                     LEPKTGYGAIVSVILDGVMTEKGRSKSADWAGRPRS"
     gene            complement(1663..4827)
                     /locus_tag="VFY96_05090"
     CDS             complement(1663..4827)
                     /locus_tag="VFY96_05090"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5605868.1"
                     /translation="MNFSEPFIRRPVMTTVLTVSAILFGILSYFRLAVNDLPAVDYPV
                     IQVQADYPGASPETVANNIATPLERQFMQINGLELVTSKSTQGHTSLTLQFVLSKSID
                     AAATDVQTAISQATGSLPVDLPSPPTFSKTNPNDQPIMYIALTSDSVTRGQLYDYGST
                     QIGQRISILPGVSRVSVFGTKSAIRIKADPSALWARGISIDDLAGAIKNGTSYLGAGQ
                     FDSENGTTLLRPQGQLDSANGYSNLIVGSSNGAPVYLRDVAQVQQSVQDERLNMRFWA
                     RGYPVPGATVVMAVYRQAGANAVEVADSIRALLPQISAELPGSVRITPLYDRSRGIVN
                     SVHDVQVTLAIAFVLVVLVIFVFLGRATDTLIPIVALPISLLLTFIVMRILGYSLDNL
                     SLMALTLAIGFLVDDAIVFLENTVRRMEHGENALQATIHSAREISFTILSMTISLAAV
                     FIPLVFMPGLVGRIFREFAITIVVSIIASGLVSLTLTPLMCARLLADRGAGSKRTWME
                     RRIGAVEQRVLTGYGKSLFWFLEHRWISALIWVVCLAGTVWLFIIVPKAFLPPGDSSV
                     VTGVFIGREGSSPEQMREIQDRVDAVLLNDPNVLATIEVTGLSQFIASNQGIMFTFLK
                     PPDARPPIQAEVAKLMGSIASIPGVMAFLRPFPVLEISTGATNQNQGQYAFSISGVNP
                     QEVYDAGTKLTAKLREFPGFLSVQSDYYNNTPGLDIEIRREQAKIHGVSEARILGLLR
                     NAYSQNYLYLIKKPSDQYQVILEAQDRARKNANDLSLLYIKSDDGKRLVPLDSLVTWK
                     SSIGPQAVNHLNQFTSVTISFNLKPGVAIGDATNFITEAANGIVPSSLRADFQGEALT
                     FRDTVRNLTILMALAVFVMYVILAILYESYLHPLTVLSTLPTALVGGLLTIYLFGEQA
                     SLYAFVGMFMLMGIVKKNGIMIVDFALQRVDAGEAAEKAIHDASMDRFRPILMTTLAA
                     VFGAIPIALGFGTDAASRRPLGLVIVGGLVVSQFITLFITPVIYLYFEDFQEKVLNRT
                     SFFRSEHGAAAIRSAREADQSEPL"
     gene            complement(4824..5984)
                     /locus_tag="VFY96_05095"
     CDS             complement(4824..5984)
                     /locus_tag="VFY96_05095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5605869.1"
                     /translation="MRAVRARSRVKHLLGFLAVLGLAAACRRAEQTTFERPPAPVTTA
                     TAVARDVPVYIDAVGKIVAREVVSIQPQVSGRITKIHFVDGANLKVGDLLFTIDPRPY
                     QAQLNQAEANLAQALAAADLAKVNFTRVENVNDPRAVSRQDYDTKKNAVATAEASVQQ
                     NRAAVETARLNLEYCTIRSPINGRAGQRTVDVGNVVSANNGSLLVIQRLDPVYADFTI
                     TESDLGAVQRNMTNRTLAVEVSLPNEDGPAREGKLTFLDNAVQDGTGTVKLRATLNNP
                     DRRFWPGRFAKVRLILNTRRDAVLIPADAPQLSAKGPFVYVVKPDSSAELRPVKLGQP
                     QGDLVVVDQGLKADERVVVTGQLGVTPGGKVRLISPQNGVKPQGTENLPAKS"
     gene            complement(5999..6424)
                     /locus_tag="VFY96_05100"
     CDS             complement(5999..6424)
                     /locus_tag="VFY96_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605870.1"
                     /translation="MILKGSNESGAPKTAAMEERIGSLFQPDTLLGEDYAANFRRKLP
                     LEPERTLLLAILEDGVRCYQENIYASGGKRRTLFEEARDWLFSDDNGWFCSFVSICTL
                     LNLEPNYIRRGLRQWESRARKTAQKKPQGAVGNDQRMVA"
     gene            complement(6479..7165)
                     /locus_tag="VFY96_05105"
     CDS             complement(6479..7165)
                     /locus_tag="VFY96_05105"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5605871.1"
                     /translation="MPNIPRLSAEARKEAIVEAVQDVFAEKGFEGTTTRELAKAAGVS
                     EALLYKHFPSKESLYAAMLDGCAKGPTFAEASRILELEASTSTLVIMVHFMISHYVLG
                     RPGDRHRAALNSLLVRSLLGDGAFVRLMHKKLASAWLKKFEACLVAAAKAGEMYEVPL
                     RRDLCVWFVQHVAFSLMLHLQPKTPAVNYRVSKEELVSQATWFGLLGIGLKQEAVRRY
                     YSPKALQLLG"
BASE COUNT         1722 a         2144 c         2043 g         1615 t
ORIGIN      
        1 tccgggacga attttgccga gatctggctc tgaaacatac gcacggaccc acttggggtc
       61 gatgatcgcc agtgaaaaaa ccggactctg cggcgtcgcc atttcacccg gttccataat
      121 tcgggttcgc accacggcat cggccggagc gatcagttca gagtcggcta actgttgccg
      181 caacaaagtc agctgggctt gggtcgcatg caaccgcgct tcgttttcgg caatatcttc
      241 tttgcgcggc ccaagcaccg ccaggtccaa agacttctgg ttgacggcga gcttcgccac
      301 ggcgacatcc aaggcggctt tcgccgcatc gacgtcctgc tggcgcacgg cgcgaccgcc
      361 ggaggcttcg gcggcatttc tgtagcgctc gaattgccgc tgggcgttca ctaggtccgc
      421 tttggcggat tcgacattgg cccgcgcctg cgcaatctcc tccggacggc tgccattgcg
      481 catgcgcgtc acaacttgaa gctgcgcggc ggattgcgct gcagcctgcg ccacttgcgg
      541 ctccaaacgg ctcgtattca atcgcgccaa caattgtccc tttcgtacgt gatctccctc
      601 ttgaaccagc acagcagcga tccgttcgct gttgttgaat gcgagctgaa cctgacgtaa
      661 gtcgatattg ccgtagagca cgagctcgcg ggccgcgcca tgccgctcaa cgatccacca
      721 aattacgcct gctgccgcaa ccaggagaat gactaagacg atcagcagcc tttttttcat
      781 acatctctcg cagtttcgct catgatcggg ggcgacctgc ccaatcggct gatttggacc
      841 tccccttctc ggtcattaca ccgtcgagaa ttaccgaaac aatcgcgcca tagccggtct
      901 tcggctctag ccccagctgt tcgatttttg ccggattcat gaccgcctgc actgctccga
      961 gcagaatttc cacgatcagt tcgctggaaa tctctttgcg gatgattcca gcccttcgac
     1021 cctgatcgaa aatctttccg aaatagcgtt gaatgagatc ccggcgccgt tcctcgacga
     1081 gctgaaacaa ttccggagtc tcgcgccgaa tatcgcgcac aaatggcggt tgaatttcct
     1141 cggtgtggcg ctgcatgcaa gcgagcagct gatgcagcgc tgccgaggca tcattggcag
     1201 agcacgtgat caccgcctcc aggtcggttt ccacgctgcg aaatttcgcc agcagaaccg
     1261 cccgcaggag ctccgtctta ctgggaaatt gcgcgtagag agtctttttg ctcatgccga
     1321 gctccgctgc aagatcgtcc atcgtgacgc tgcgaaatcc atgcgcaaaa aaatgcgctc
     1381 gcgcagccaa gacgattcgc tccgcgattt cgttcaatcg ctgggccggt ggtccggtgc
     1441 gggtcgggtg cttcgaagcc gaagtagcca tgataccaag gaaactatac tagtatttac
     1501 agtttccgcg ccataaaagc aagcaaacga gatccgacct cgactaggcg gaggctctga
     1561 gcacacgagc aggcgtttca tgcggtagag tcggttggta cgaacgagaa aactagacag
     1621 cgcggtggtg tcaccgccgt tgaacggcac aggcgagttt gcctaaagag gttcactttg
     1681 atccgcttcc cgggcagatc ggatggctgc ggcgccatgc tcggaacgaa aaaaagaagt
     1741 gcggttcaat accttttcct ggaaatcttc gaagtagagg tagatcaccg gagtgatgaa
     1801 caaagtgata aattgcgaca ccaccaaccc gccgacgatc acaagcccga gaggccttcg
     1861 tgatgcggca tccgtgccga acccgagcgc gatcggaatt gcgccgaata ctgcggcaag
     1921 agtcgtcatc aaaatcggtc gaaaacgatc catgctggca tcgtgaatcg ctttttctgc
     1981 cgcttcgccg gcatcaaccc gctgcagtgc aaaatcaacg atcatgatac cgttcttttt
     2041 gacaatgccc atcaacatga acatgccgac gaacgcataa agtgaggcct gttcaccgaa
     2101 caaataaatc gtcagcaagc cgccaacgag cgcggtcggc aaggtcgaaa gcaccgtcaa
     2161 cggatggaga tagctctcat aaagaatcgc caaaatcacg tacatgacaa agaccgctaa
     2221 ggccatcaaa atcgtcagat tgcgcacagt gtcgcgaaac gtgagcgcct caccctgaaa
     2281 atcggcgcgc aagctcgatg gcacgatgcc attggctgct tcagtaatga agtttgtagc
     2341 atcgccaatc gctactccgg gcttaagatt aaacgaaatc gtcacgctgg tgaattgatt
     2401 gagatggttg accgcctgag gcccaatcga agatttccaa gttaccagag agtccagtgg
     2461 aacgagccgc ttgccgtcat ccgatttgat atagagcaag gacaagtcgt tggcattttt
     2521 ccgcgcgcga tcctgcgcct caagaatgac ctgatactga tcggatggtt ttttgatgag
     2581 atagagatag ttttgcgagt aagcgttgcg tagcaaacct aaaatccgcg cctcggaaac
     2641 accgtgaatc ttcgcctgct cacggcgaat ttcgatatcg aggcctggcg tgttgttgta
     2701 gtagtcggat tgtacgctaa gaaatccggg gaactcacgc agcttcgccg tgagcttcgt
     2761 gccggcgtca tacacttctt gcggattgac cccggaaatc gagaatgcgt attgtccttg
     2821 attttgattg gtcgctcccg tgctgatttc aagcacagga aacggtcgga gaaatgccat
     2881 cacgcccgga atcgaagcga tgcttcccat cagcttcgcg acttccgctt ggatcggcgg
     2941 acgcgcgtca ggcggtttta gaaaggtaaa cataataccc tgattggagg cgataaactg
     3001 actcaagccg gtgacttcga tggtggccaa tacgttggga tcgttcagaa gaaccgcatc
     3061 gacgcgatct tgaatttcgc gcatctgttc cggcgatgat ccttctcggc cgataaagac
     3121 cccggtgacc acgctgctgt ctcccggcgg caaaaatgct ttgggcacga tgataaacaa
     3181 ccatacggtc cccgcgagac aaacaaccca gatcagcgcc gaaatccaac gatgctcgag
     3241 aaaccaaaaa agggattttc cataaccggt aaggacacgc tgctcgacag cgccgatgcg
     3301 ccgctccatc caggtgcgct tcgatcctgc gccgcggtcg gccaacaatc gcgcgcacat
     3361 caacggagtg agggtcaaag aaaccaggcc ggaggcgatg attgagacca cgatcgtgat
     3421 cgcaaactca cggaatattc ttcccaccag gccgggcatg aaaaccagcg ggatgaacac
     3481 cgcggcaagc gaaatggtca tggaaagaat ggtaaaactt atctccctgg cgctatgaat
     3541 tgtcgcttgc aacgcgttct cgccgtgttc catgcggcgc acggtgttct ccagaaacac
     3601 aatcgcatcg tcgaccaaaa acccaatggc caaagtgagc gccatcaacg aaagattgtc
     3661 gaggctatat cccagaatgc gcatgacgat gaacgtcagc agcaaagaaa tcggcaaggc
     3721 cacgatcgga atcagcgtgt cggtggcacg tccgagaaat acaaaaatga ccaggacgac
     3781 cagcacaaat gcgatcgcca aagtgacctg aacatcgtgc accgagttga cgatgccgcg
     3841 agaacggtca tacagcgggg taatccggac cgatcccggc agctccgcac tgatctgggg
     3901 cagcaaagcg cggatgctat cggccacctc caccgcgtta gcgcctgcct gacggtaaac
     3961 ggccatgaca accgtcgcgc cgggcacggg atatccgcgt gcccaaaaac gcatgtttag
     4021 ccgctcgtct tgcaccgact gctgcacctg agcgacatcg cggagataga cgggcgcccc
     4081 attgctcgag ccgacgatca gattgctgta gccattggcg ctatcaagct gcccctgcgg
     4141 gcgcagtagc gtagtaccgt tctcggaatc gaactgcccg gcgccgagat agctggtacc
     4201 attcttgatc gctcccgcca aatcgtcgat cgagatcccg cgcgcccata atgcggaggg
     4261 atccgctttg atgcggatgg ccgatttcgt gccaaaaaca ctcacccgag aaacgcccgg
     4321 aagaatactg atccgctgtc caatctgcgt gctgccgtaa tcgtaaagct gcccgcgggt
     4381 caccgaatcg ctggtcagcg caatgtacat gatcggttgg tcattagggt tggttttgga
     4441 aaaggtcggg ggcgatggca ggtccaccgg caagctaccg gtggcctgcg atatcgcggt
     4501 ttgtacgtcg gtggcagcgg cgtcgatgct tttactaaga acaaactgta gcgtcagact
     4561 cgtgtgccct tgcgtgctct tggaggtgac gagctcgagc ccattgatct gcatgaactg
     4621 ccgctctaac ggcgtcgcga tattgttggc gacggtttcg ggactggcgc caggataatc
     4681 cgcctgtacc tggatgacgg gataatctac cgccggcaaa tcgttcaccg cgagccgaaa
     4741 ataactgaga ataccgaaaa gaattgccga gacggtcagg acggtggtca tgaccggccg
     4801 gcgtatgaaa ggttcagaaa agttcacgat tttgccggca agttttccgt cccctgaggc
     4861 ttcacgccat tttggggtga gatcaagcgc accttgccgc caggcgtaac gccgagctgt
     4921 ccggtgacga cgacacgctc atcggccttg agaccttggt cgaccacaac aaggtcaccc
     4981 tgcggctgac ccagcttgac gggtcgcaac tcggcactcg aatcgggttt gacaacgtag
     5041 acgaaaggcc ccttggccga aagctgcggc gcatccgctg gaatcaacac cgcgtcgcgc
     5101 cgggtattca agatgagtcg aactttcgca aatcggccag gccaaaaccg gcgatcgggg
     5161 ttgttgagcg tcgccctaag cttcaccgtg ccggtgccgt cttgcacggc gttgtcgaga
     5221 aatgtcagtt tgccctcccg cgccggtccg tcttcgttgg ggagactgac ttcaactgct
     5281 aacgttcgat tggtcatatt gcgctggaca gcgccaaggt cactctcggt tatagtaaaa
     5341 tcggcataga ctgggtcgag cctctggatc actagaagtg atccgttatt cgcagagacc
     5401 acattgccga catcgacggt tcgctgcccg gcgcgaccat taatcggcga tcgaattgtg
     5461 cagtattcga gattcaatcg cgccgtttcc actgcggcgc gattttgctg gacactcgct
     5521 tccgcagtgg ctacggcatt tttcttggta tcgtagtctt gacgggatac ggcgcgcggg
     5581 tcattgacat tttcgacccg ggtaaaattc accttggcta gatcagccgc tgccagggct
     5641 tgcgccagat tggcctctgc ctggtttagc tgcgcttggt agggacgcgg atcgattgtg
     5701 aaaagcagat cccctacttt gagattcgcg ccgtcgacaa agtgaatctt agtgatgcgc
     5761 ccagaaacct gcggctggat cgacacaact tcacgggcaa cgatcttccc cacggcgtcg
     5821 atatagactg ggacgtcacg cgccaccgca gtggccgttg tgaccggcgc gggcggacgt
     5881 tcgaacgtgg tctgttccgc gcgccggcaa gctgccgcta gcccaagcac cgccaaaaac
     5941 ccgagcaggt gtttaacgcg tgaacgagcc cgtaccgcgc gcatcacgag gtttcacctc
     6001 aagcgaccat gcgctgatcg tttccaaccg ctccctgagg ttttttctgc gctgtcttgc
     6061 gagcccgtga ttcccattgc cgcaaaccgc gacgaatgta attaggctcg agattcaata
     6121 gggtacagat ggaaacaaat gagcagaacc atccgttgtc gtcgctaaac agccaatcgc
     6181 gtgcttcctc gaagagcgtg cgccgcttgc caccggacgc gtagatattt tcctgataac
     6241 agcgaacgcc gtcttcgaga attgccagca agagggttcg ctctggttcg agcggcagct
     6301 tgcggcggaa attcgctgca taatcttcgc ctaataatgt gtccggctgg aacagcgaac
     6361 cgattctttc ttccattgcc gccgtttttg gcgcgccgct ttcatttgag cccttgagta
     6421 tcattcgccc tccgtaatgg tgtaatagcc gattagtaaa ttcttactta ctaaatagtt
     6481 agcccagcaa ctgcaatgcc ttcgggctgt agtagcgtcg taccgcctct tgcttgaggc
     6541 cgataccgag caatccgaac caggttgcct gggaaaccag ctcttcttta gaaaccctgt
     6601 agttgaccgc cggcgtcttc ggttgtaaat gcaacatcaa cgaaaacgcc acgtgttgaa
     6661 caaaccagac gcaaagatcg cggcggagcg gcacctcgta catttccccg gccttcgccg
     6721 ccgcaactaa acaggcttcg aatttcttga gccaagccga cgcgagtttc ttatgcatca
     6781 gcctaacgaa cgcgccgtcg cccagcaagc tgcgcactaa taaagaattc aatgctgcac
     6841 ggtgcctatc tccagggcga cctagcacgt aatgtgagat catgaaatgg accatgatga
     6901 ccagggttga agtcgatgct tccagctcta ggatccggct cgcctccgca aatgtcggcc
     6961 ccttcgcgca cccgtcgagc atggccgcat acaacgattc tttgctggga aaatgcttgt
     7021 aaagaagcgc ttctgaaacc ccggcagcct tggccaattc gcgggtggtc gtgccctcga
     7081 aacccttttc ggcaaacaca tcctgcaccg cttcgacaat cgcctctttg cgggcctcgg
     7141 cggagagtct cggaatgttt ggcaccaagg ttagttagca tttactaacc aaacgtgtca
     7201 aggagattta gctgaacagg ctaaagaatt tagctcaggg gcgcgctacc ccggactgga
     7261 tccggttgcc ggaaatgaca aggtattttt tgcctacctc aattccagga cacttgcgtg
     7321 tccttggaga gggttccgag agatctttct ctgataaaaa tttatatgtg ccccaggtga
     7381 agccttgtcc ggacgcgaga tttctaattg cgcaggaaat ccgtccagcg cttcttacat
     7441 tgaaccgcca cctgaagtct ttcgaggtgt tgctccagct gtgtactgcc ctgtttcacc
     7501 ggatgagccg cgaagaattc gcgc
//
LOCUS       DASULZ010000248         4845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1450280, whole genome shotgun sequence.
ACCESSION   DASULZ010000248 DASULZ010000000
VERSION     DASULZ010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450280"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1119)
                     /locus_tag="VFY96_05110"
     CDS             complement(28..1119)
                     /locus_tag="VFY96_05110"
                     /inference="COORDINATES: protein motif:HMM:NF045247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5605872.1"
                     /translation="MNCVVPNELVVERGSELSLVFNNLRSTLPQVPVEIVDDVRMAAA
                     HERSVDQFGAAKRKLFLTRYKGEFFKKCPGSDGQVCCNYFVINFASNCPMDCSYCYLQ
                     EYLSDNASLKVFSNIDDLIDEADRTLRKHRGLFFRIGTGEITDSLALEPYTGIVREMI
                     PYFAEQSNVLLELKTKSDCVDNLLDLDPKGRIVVAWSMNPRSVIERDEQGTASFAERL
                     AAARRCQEAGYRLGFHFDPMIEYPDWENDYQSMVEEIFATIDWRRVSWLSLGVLRETP
                     SLKRVMRQRFPRTRLLVGEQVLCPDGKLRYFQPQRIQMYRKMVRWIRQFAPTVQIYLC
                     MESKEIWQQVFGFAPSCEKELGNQLAPVD"
     gene            complement(1116..1610)
                     /locus_tag="VFY96_05115"
     CDS             complement(1116..1610)
                     /locus_tag="VFY96_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605873.1"
                     /translation="MERWLRWAAADREALATLALSHKISENHLREMMDWLEEISLRDG
                     KPIQDILAEKAIYDTSTDPRLGRADRLKRIKEQLRRRRYPGLAQTEDQIRTHINALKL
                     HPEIRLSVMPGLEGGHLKIEIQPANIAELRAAVAKLSNATSLRPLTAIFELLSGDGAE
                     EQTK"
     gene            complement(1594..2499)
                     /locus_tag="VFY96_05120"
     CDS             complement(1594..2499)
                     /locus_tag="VFY96_05120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015337578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX5605874.1"
                     /translation="MVPRVSVVIPSFNRWPLLAEAVNSVLAQSYRDFEVIVVDDGSTD
                     ATASQLAKLASGLKLLMTERRGVSAARNLGVSQSRGQYIAFLDSDDLWLKRKLERQTR
                     FMDEHPEFHICQTDEIWIRNGVRVNPRAIHQKTSGDIFKRSLELCLVSPSAVMMTKAL
                     FDRTGGFDEAFPVCEDYDLWLRITAQHQVALIPDRLTIKRGGHPDQLSRSVWGMDRYR
                     ILALQKLLRSNLDRTQQAAALEMLRQKVAIVAAGARKRGREQDAQSLEAIVAEFEQEK
                     VHGRERDPSLREPEGISTADCGTLA"
     gene            complement(2542..2973)
                     /locus_tag="VFY96_05125"
     CDS             complement(2542..2973)
                     /locus_tag="VFY96_05125"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HEX5605875.1"
                     /translation="MKIEGSYIFSAPRQRVWEVLLDPKVMAQCMPGCESMNEVAPDQF
                     EAVMKVGVASVKGTYKGKVAIKDKQPPSHYVLSGQGSGGPGFMQGDVAIDLEEAGGQT
                     VLKYSTDAKVGGLIASVGQRMLNGVAKMMVDQFFKKVESFI"
     gene            3578..>4845
                     /locus_tag="VFY96_05130"
     CDS             3578..>4845
                     /locus_tag="VFY96_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5605876.1"
                     /translation="MQAQIREAQGDPIRAHALYNTVRDSAPNSRWAAAARKAQIGLRQ
                     TFPDLFTFQTTDELAAEADRLARERQSFEAETLYKKLLNNASDPASQLTYLTKLANLF
                     FSTRSRNDSIPTLELIARDFPDSPEAPKALYQIGQIYWNRHENTQALEYFQLILEKYP
                     TSESVDRARFAAADIHEYFGRRPEAIQLYSDMIKLSPVGQIRDDAAWRLAWLYYRTSD
                     LVAASETFSVLAKQSANGSFGTAALYWLGRIAEKSGEYDKAKQIYRQIAATATESYYQ
                     TLSWRALQRLGDAGEEPKWVVASSPPEADDPPIPPELLFHLSRARALTSLSLQHLAIP
                     ELEELNRGANPSNRMRAFLMREYFKSGAYARSLQLANQLPSSRRDREPYRYPLAFWEL
                     IQKKAQERGLDPYLVVALIRQESLFDTRARS"
BASE COUNT         1134 a         1420 c         1163 g         1128 t
ORIGIN      
        1 tctcgtcaga ctagccattt gcatgtatca atcgacgggt gccaattggt tgccgagctc
       61 tttttcacat gagggcgcaa aaccgaagac ctgttgccat atttcctttg attccatgca
      121 aagataaatc tgcaccgtcg gtgcaaactg gcgaatccat cgaaccattt tgcggtacat
      181 ctgaatacgc tgtggctgaa agtagcgcag cttgccgtcg ggacagagca cctgctcacc
      241 gacgagtagc cgtgtacgcg ggaagcgttg gcgcatgacg cgtttgagac tgggagtctc
      301 gcgcaagacg ccgagactca gccaggaaac tctacgccaa tcgatggtgg caaagatttc
      361 ctctaccatt gattgataat cgttttccca atcggggtac tcgatcatcg gatcgaagtg
      421 aaatccgagt cgatagcccg cttcctggca gcggcgcgcc gccgccagcc gttcggcaaa
      481 agacgccgtg ccttgttcat cgcgctcgat gacggatcgt ggattcattg accacgccac
      541 gacgattcgg ccctttggat cgagatcgag tagattgtcg acgcagtcgc tctttgtctt
      601 tagttccagc aacacgttgg actgttcggc aaagtacgga atcatttcgc gcacgatgcc
      661 ggtgtatggc tccagcgcaa ggctatccgt gatctccccc gtgccgatgc gaaaaaaaag
      721 cccgcgatgt tttcgcaatg tgcgatcggc ttcatcgatc aagtcgtcga tgttgctgaa
      781 cacttttagc gaagcattat ccgacaaata ctcttgcaaa taacagtaac tgcaatccat
      841 cgggcagtta ctcgcaaagt tgatgacaaa atagttacag cagacttgcc cgtcgctacc
      901 cggacacttt ttaaagaact cgcccttgta ccgagtcaaa aaaagcttcc tttttgccgc
      961 gccgaactga tcaacagaac gctcgtgtgc cgcagccata cggacgtcgt cgacaatctc
     1021 gacgggaacc tgcggcagcg tggaacgcaa attgttgaag acaagactta attcggaacc
     1081 gcgttcaacg actagttcat tcgggacaac acaattcatt tagtttgctc ttccgcgccg
     1141 tctcccgata aaagctcgaa aatcgccgtt agcgggcgga gcgacgtggc gttgctaagc
     1201 ttcgccacgg cggctctgag ctcagcgata ttagccggct ggatctcgat cttgaggtgg
     1261 ccgccctcca ggccgggcat gacgctcagg cgaatttctg gatgaagttt cagggcattt
     1321 atatgagtgc gaatctggtc ttccgtttgg gccaaacccg ggtatcttcg ccgtctgagc
     1381 tgctctttga ttcgcttcaa ccgatcggcg cggcccagcc gcgggtctgt gcttgtatcg
     1441 taaatcgcct tttcggcaag aatatcttgg atgggtttac cgtctcggag cgaaatctcc
     1501 tcaagccaat ccatcatctc gcgcaagtga ttctcactga ttttgtggct caacgcgagc
     1561 gttgccagcg cctcccgatc cgccgccgcc catctaagcc aacgttccac agtctgccgt
     1621 tgaaatcccc tctggttcgc gtaagcttgg atctcgctct ctcccatgca ctttctcctg
     1681 ctcgaattcg gcgacaatgg cttccaagct ttgcgcgtct tgctcccgcc cccgctttcg
     1741 agcaccggcg gcaacaatcg ccactttctg ccgcagcatt tccaacgctg ccgcctgctg
     1801 agtgcggtcc aaattcgacc gcagtaattt ctgcaacgcc aaaatccggt aacgatccat
     1861 tccccacacg gatcgcgaca actgatcggg atggccgccg cgtttgattg tcagccggtc
     1921 gggtatcagt gccacctggt gctgcgcggt gatccgaagc caaagatcgt aatcttcgca
     1981 caccggaaat gcttcatcga aaccgccggt tcggtcaaag agggctttgg tcatcatcac
     2041 ggctgatgga ctgaccagac aaagttccag gctcctctta aaaatgtcac cggacgtttt
     2101 ttggtgaatc gccctgggat taacccgaac tccattacga atccagatct cgtccgtctg
     2161 acagatgtga aattccggat gctcgtccat aaaccgagtt tgtctctcga gctttctctt
     2221 gagccaaaga tcgtccgaat ctaagaatgc gatatactga ccgcgagatt ggctcacacc
     2281 tagattacgc gccgccgata cccctcttct ttctgtcatc aaaagtttca gccctgacgc
     2341 cagcttggct aattggctgg ctgtagcatc ggtggatccg tcatcgacga caatcacctc
     2401 gaaatcgcga tagctttgcg ccagaactga attgaccgct tctgcgagca gaggccaacg
     2461 attaaagctt ggaataacta cgctgacgcg cggcaccata acactagacc gcttgatctg
     2521 acgatggatt ctcgaatgag cttagatgaa agattccact ttcttgaaaa actgatccac
     2581 catcattttg gccacaccat tgagcattcg ctgtccgacg ctggcgatca gaccgccgac
     2641 cttcgcatcc gtgctgtatt taagcaccgt ttgtccgcca gcctcttcta aatcgatcgc
     2701 gacatcccct tgcataaatc cgggaccgcc tgacccttga ccgctcagaa cgtaatgcga
     2761 aggaggctgc ttgtccttta ttgcaacctt ccccttatac gtccccttca ccgatgccac
     2821 gcccactttc atcactgcct cgaactgatc cggcgccact tcgttcatgc tttcgcagcc
     2881 tggcatgcac tgcgccatga ccttgggatc cagcaaaacc tcccagaccc gctgccgcgg
     2941 cgcactaaat atataacttc cttcgatctt caatttatcc tccccgggtt tagacgtgct
     3001 tcttaatggg cgcgcgaccg gatcgatgat cgaccgcaaa aagataactt ccagtcggtg
     3061 aaaacacaac cctgaggacc gacggattca atcaaacgcc cgcgtatttg attgaatccg
     3121 atctccacct tggctaaaat ttcataaatt gatgagacac cgattcactg ttctgctgct
     3181 gattctcgct ctcggagccg tcccggcgtc tatttttggc caaacgagcg atgcaaacga
     3241 aatattcgca aaagcttacc gactttacac gaagggcaaa acctcggatg ccaaagaact
     3301 tctcgaaaaa attactgatc ccaagtttcg cttagccgac tacagccaat actatcttgc
     3361 tctcataaac tacgatctga ccaatcggga gcaagcacgc cagatcctaa tccaattgat
     3421 acagcgtttc ccgcaaagca tttggattcc aggcgcgact ctgcttcaag ctaaaatcga
     3481 catcgccgaa aaaaactacg gctccgcgga agaaattcta cgtcagctca gggcggacaa
     3541 atcggcaaaa cgtgagatca ccgacgaagc tctctacttg caggcacaga ttcgggaagc
     3601 tcaaggcgat ccgatccgcg ctcacgcgct gtacaacaca gtccgcgact ccgcgccgaa
     3661 ttcccgctgg gcagcggccg ccagaaaagc gcaaatcggc ctacgacaaa catttccgga
     3721 tctcttcacg tttcagacga ccgacgagtt agctgcggag gcggaccgct tggcgcgtga
     3781 acgccaaagc tttgaggctg agactctata taaaaagctt ctgaacaacg cttcagatcc
     3841 tgccagtcag ctgacctatc tcaccaaact ggcgaatctg ttcttctcga cccgcagtag
     3901 gaacgactcc attccgactc tcgagttgat tgcgcgagat tttcctgaca gcccggaagc
     3961 ccctaaggcg ctctatcaga tcggccagat ttactggaac cggcatgaaa acacacaagc
     4021 cttagaatac ttccagttga tattggaaaa atatccgacc agtgagtccg tggatcgcgc
     4081 gcgttttgcc gccgccgaca tccatgagta tttcggcaga cggccagagg cgatccagct
     4141 ctacagtgat atgattaaac tgtctcctgt aggccagatt cgtgatgacg cggcatggcg
     4201 actggcctgg ttgtactatc gcaccagcga cttagtcgcc gcgtccgaaa ccttcagcgt
     4261 gctggcaaaa caatccgcca acggttcatt tggtaccgca gctctttatt ggctagggcg
     4321 gatcgccgaa aaatcgggtg aatatgacaa ggccaagcaa atttatcgtc aaatcgccgc
     4381 caccgccaca gagtcgtact atcaaacgct gtcctggcgt gctctacagc gtcttggtga
     4441 tgccggcgaa gagcccaaat gggtagtggc gagctccccg ccggaagccg atgacccgcc
     4501 gattccgccg gagctgttgt ttcatcttag ccgtgcccgt gccctcactt ctctgtcttt
     4561 gcaacactta gctatcccgg agctggaaga gctcaatcga ggcgccaacc caagtaatcg
     4621 catgcgtgcc tttctcatgc gcgaatattt taaaagcggt gcttacgcgc gcagccttca
     4681 attagcgaac cagttgccga gctcacgccg cgaccgtgaa ccctatcgct acccgctggc
     4741 gttctgggaa ttaatccaaa aaaaagccca agaacgcggg cttgatccgt atctcgttgt
     4801 ggcactcatt cgtcaggaaa gcttgttcga cactcgtgcg cgatc
//
LOCUS       DASULZ010000249         4432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_740456, whole genome shotgun sequence.
ACCESSION   DASULZ010000249 DASULZ010000000
VERSION     DASULZ010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740456"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VFY96_05135"
     CDS             complement(<1..363)
                     /locus_tag="VFY96_05135"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5605877.1"
                     /translation="MPKKQIVQVNLAAANPNLSPATRFGNLVFVAGQTGRHPVTGEVG
                     RDVREQTRYAIERIKMILETAGTSLDNVLTVLTHLTRREDLAAYNEEYGKFFQTDKPA
                     RTTVEAMLNFPELLVEITV"
     gene            complement(555..1394)
                     /locus_tag="VFY96_05140"
     CDS             complement(555..1394)
                     /locus_tag="VFY96_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5605878.1"
                     /translation="MNLTAYNQVSIPNFMYGTAWKKAATEELVQLAVASGFRAIDTAN
                     QLIHYEEALVGEALQALEQQGVRRETLFLQTKFTSVDGQDHRTPYDPTADLTTQVKQS
                     FDSSLIHLGTAYLDSYVLHAPFQRRGLGESDWEVWAVMEQLYESGKTKMIGISNVAAG
                     QLVELCEKARHKPMVVQNRCFAALGWDKQVRDICQAQNIIYQGFSLLTANREVLVDPE
                     IRTIARRLGAGIAQVIFRFAMQIGMLPLTGTTSEQHMKEDLQAEQFTLSAEEIERIET
                     IAI"
     gene            1833..1985
                     /locus_tag="VFY96_05145"
     CDS             1833..1985
                     /locus_tag="VFY96_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605879.1"
                     /translation="MATQKQRRAARKNIKKAATAAKRKQTLKHLPKKTRRALGQQAAK
                     ARQSRH"
     gene            complement(2073..2903)
                     /locus_tag="VFY96_05150"
     CDS             complement(2073..2903)
                     /locus_tag="VFY96_05150"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX5605880.1"
                     /translation="MRMIDLTMPIWEGAGYGEILPFTNSSVRFSEYMYYDKHGLRMTR
                     MKLDGETGSPLMVPHQRMPFDPTPLQPDPKFSWTLDQIPLDRLILRDTVILDVRAPEQ
                     HEITLDEMDQAIKSADFRKEDEVLIRTGWGTRARAYELGLDYYKRTPSIHFDAAALLA
                     KKMDEMGSGIFMTDCGLVNPPRVQGNNWFRGENPIIPHPKPWPSAEARERVLDLGAHR
                     HGSPHPSSYGALIRKSIAGCKCLVDCDRISKPRVKMIILPLFIKQGGASPCRFVAIEE
                     "
     gene            complement(2905..3672)
                     /locus_tag="VFY96_05155"
     CDS             complement(2905..3672)
                     /locus_tag="VFY96_05155"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX5605881.1"
                     /translation="MNFVDLTMPLNHRWMPDEGLPTAIKFFLGPKDHQEKGMVLGSDS
                     GTSLALPSLFAEFRKTTRLDEIPIEKLFLRPTAVAHIDKADEKQISKSDVEKAFNDTG
                     PAKGDAFLIVTGWGDKGHYQGEGNEYILGSPHFSFEAARTLAQALHENENDLLLTDTA
                     VLGLPKSHIIPEWFSMVPAPSAESAEARMYLHLYDSDKAKKDFAVEMEFARLGITTVR
                     KLIQCGGIQRQRIKLMVSPLNVVRGVASTCRVVAVEE"
     gene            complement(3894..>4432)
                     /locus_tag="VFY96_05160"
     CDS             complement(3894..>4432)
                     /locus_tag="VFY96_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605882.1"
                     /translation="RDNINIQAIGDSTVIAQSLATGIIDAAALDKLQSVRLQGLGVKV
                     LFDLSRIAFPSSPFMGAEPFIEKNPQVIEKFIKALIEASTVMRAQKERGLAVLQRHIK
                     TDRALAEIGYKNLLDDLTAYTFTTVKGLRTVQEIVALRDPKIGKYNVEDLLDQRILKK
                     VVDSGYVEEVERRYKIRS"
BASE COUNT          975 a         1263 c         1157 g         1037 t
ORIGIN      
        1 gaccgttatc tccacaagca gttcgggaaa gttcagcatc gcctccacgg tcgtcctcgc
       61 gggtttgtcg gtctgaaaaa acttgccgta ctcctcgttg taggccgcaa gatcctcacg
      121 tcgggtgaga tgcgtcaata cggtcagaac attgtcgaga gatgtccctg cggtttccaa
      181 aatcatcttg atgcgttcga tcgcgtaccg cgtctgctcg cgcacgtctc tgccgacctc
      241 tccggtaacc gggtgccggc cggtttgacc ggctacgaac accagatttc cgaaccgcgt
      301 ggccggtgaa agattcggat tcgccgcagc aagattcacc tgaacgattt gtttcttggg
      361 catctcatct cctgaatcaa acttcgctcg ccggcagggt tgtggcaggt ggaccaacct
      421 gtgttcttcg caagtgccac tcgcaatcca accgcagcat cacggatgaa aatttctgct
      481 ttcgtttgcc gaacgtgtga acggagtaaa ccgattcaac agcttgaacg ctttgaacag
      541 agcaacgcga ttgattagat ggcgatggtt tcaatccgct cgatttcctc agcagagagt
      601 gtgaactgct cggcttgaag gtcctctttc atatgctgct cgctcgtggt gccggtcagc
      661 gggagcatgc cgatttgcat ggcaaaccgg aagatgacct gtgcgatgcc tgcccctaac
      721 cgccgagcga tggtacgaat ttccggatcc accaggactt ctctgttggc cgtcaataac
      781 gagaagcctt gatagatgat gttctgagct tggcaaatgt ccctgacctg tttgtcccag
      841 ccgagcgccg caaaacagcg gttttgtacg accatcggtt tatggcgcgc cttttcacac
      901 agctctacca gctgaccggc cgcgacattg ctgatgccga tcattttggt tttccccgat
      961 tcataaagtt gttccatcac cgcccacacc tcccagtccg actcccccaa accgcgccgt
     1021 tgaaatggcg cgtgtaatac atacgaatcc aaatacgctg tgccgaggtg gatcaacgag
     1081 ctgtcaaacg actgtttgac ctgcgttgtt agatcggccg tgggatcgta cggcgttcga
     1141 tggtcttggc catcgaccga agtaaatttg gtttggagaa acaaggtctc tcgcctgacc
     1201 ccttgctgct cgagcgcctg caaagcttcg cccaccaatg cttcctcata atggatcagt
     1261 tgatttgccg tatcgatggc cctgaatcct gaggccacgg ctagttgcac cagttcctcc
     1321 gtggccgctt ttttccatgc cgtgccatac ataaaattag gaatagaaac ctggttatag
     1381 gcagtcaaat tcataacgtt ccttcagatc gggattaatc aaacatttca cgatcggcgc
     1441 gatgcctccg cggtgttggc tcgattttat accgaaattt tcgagttcaa ggaagacgac
     1501 aacgttctag gatcaaactg gctggtgcga tgataaatcg caccaggaaa tttcgcgcga
     1561 agtagccgcg acgtgcgctt acctaatcga agaaaattgc agtggacgac ctgaccacac
     1621 gctcgagctc gggcgcgtta atacggcggc tgctcggttt gaattcgcga caccagcgcg
     1681 gagcgggtga cacggaacga acgatgggag gccgcgtcgc tcggctgccg catttgcgtc
     1741 aagcgaaatt tgaaaatctc ggcaacttct ttgaactgaa gctcgtcctt tttaggctga
     1801 aatcggaatt ttaaaagagc ttaggaggca ttatggctac gcagaagcag cgccgggcag
     1861 ctcggaagaa catcaaaaaa gccgccacgg cggcgaaacg aaagcagact ttgaaacatt
     1921 tgccgaagaa aacgcggcga gcgctcggac aacaggccgc gaaagccaga caatcacgcc
     1981 actgaggcgc gctcgccctt ctgactcgga agacgtgtca agcgcgctct ccggctctcc
     2041 acacatgccg gatgcacccg gcttaaacaa tttcattcct cgatcgcgac gaaacggcac
     2101 ggcgatgcgc cgccttgttt aatgaacagt ggaaggatga tcatctttac cctgggcttc
     2161 gagatcctgt cgcaatccac gagacacttg cagcctgcga tggattttct gatcaacgcc
     2221 ccgtagctgc tcggatgcgg actgccgtgc cggtgcgcgc ccagatcgag cacgcgctcc
     2281 cgtgcttcgg cggacggcca tggcttggga tggggaatga tgggattttc gccgcgaaac
     2341 cagttgttgc cctggactct gggcggattc accaagccgc aatcggtcat aaaaataccg
     2401 ctccccatct catccatttt cttggccaat agcgcggcgg cgtcgaaatg gatgctcgga
     2461 gttctcttgt agtagtcgag cccgagctcg taggctcgcg cgcgcgtacc ccagccggtg
     2521 cgaatcagca cctcgtcttc tttgcggaaa tcggcgcttt tgatcgcctg gtccatttcg
     2581 tcgagcgtaa tctcgtgctg ttccggtgct ctgacatcca ggatcaccgt atctctgaga
     2641 atcagtcgat cgagcgggat ctggtcgagg gtccatgaaa acttcgggtc cggttgcaaa
     2701 ggcgtggggt caaacggcat gcgctgatgc ggcaccatca aaggactgcc ggtttcgccg
     2761 tcgagtttca tgcgcgtcat ccggaggccg tgcttgtcat aatacatgta ctccgaaaac
     2821 cgcaccgaag agttggtaaa gggcaaaatt tctccgtagc cggcgccttc ccagatcggc
     2881 atcgtcaaat cgatcattcg catgtcattc ctccacggcg acgactctgc aagtcgaagc
     2941 gacgccgcga acaacgttca acggcgagac catcaacttg atccgctgac gttggattcc
     3001 gccgcactgg atgagctttc gcacggtcgt aatccccaac cgggcaaatt ccatctcgac
     3061 ggcgaaatct tttttcgcct tgtcgctgtc gtatagatgc agatacatgc gcgcttcagc
     3121 cgactctgcg gacggagccg gaaccatcga gaaccattcc ggaatgatat ggctcttcgg
     3181 cagcccgagc actgccgtgt cggtgagcag caagtcattt tcattctcgt gcagcgcttg
     3241 cgccaatgtt cgcgcagcct caaacgagaa atgggggctg ccaaggatgt actcgtttcc
     3301 ttctccctga taatgccctt tgtcccccca acccgtgacg atcaaaaatg catctccttt
     3361 cgctggaccg gtgtcgttaa acgccttctc aacgtccgac ttgctgattt gtttttcatc
     3421 agctttgtcg atgtgcgcca ccgcagtcgg ccgcaaaaac aacttctcga tcggtatctc
     3481 atcgagccgc gtcgtcttgc gaaactcggc gaacagcgaa ggcagcgcca ggctcgttcc
     3541 cgaatcgctc ccgaggacca tgcctttttc ctggtgatcc ttaggcccga ggaaaaattt
     3601 aattgctgtc ggcaacccct catcgggcat ccagcgatgg tttaatggca tcgtcagatc
     3661 gacaaaattc ataattcctc cactttgctc agcgcgtctc gtgatagaga aaccgtaaag
     3721 cctcggaaca aaggggtcgc gggaagcctg ccaggaggct tctcgaccgg ccgaagcttg
     3781 gaacaaccat aacgagccgc actaatggaa gacgttcgaa cggagcggaa cgttaaacga
     3841 tgtgagcggc ttgaagggct ttcacgcttg gcattcccaa ttccctgacg acgctaagat
     3901 cgaatcttgt atctgcgctc gacttcctcc acatagccgc tatcaacgac ctttttgagg
     3961 atgcgctgat ccaacagatc ctcgacattg tatttcccga tcttgggatc tcgcagcgcg
     4021 acgatctcct gtacggttct caagcccttc acggtggtaa aggtataagc cgtcagatcg
     4081 tccagaagat ttttgtatcc tatctccgcg agcgccctgt ccgttttgat atgtctttgc
     4141 agaaccgcga ggccgcgttc tttttgcgcg cgcataaccg tgctggcctc gatcagcgct
     4201 ttgataaatt tctcgatcac ctgcggattt ttttcgatga agggctccgc gcccataaaa
     4261 gggctcgaag gaaaagcgat ccggctcaga tcgaaaagga ccttaacgcc caggccctga
     4321 agccgcacgg attgcaattt atccaacgcc gccgcatcaa tgattcccgt cgccagagac
     4381 tgggcgatca cggtactgtc gcctatggcc tgtatattga tgttgtccct ct
//
LOCUS       DASULZ010000250         2175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1147763, whole genome shotgun sequence.
ACCESSION   DASULZ010000250 DASULZ010000000
VERSION     DASULZ010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147763"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(533..1021)
                     /locus_tag="VFY96_05165"
     CDS             complement(533..1021)
                     /locus_tag="VFY96_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019719543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEX5605883.1"
                     /translation="MKGLSEKLIVSHHQNNYSGVVRRLNQIPQQIGGLPKGAQPYQMG
                     SPKQEEIIATNSMILHEFYFDNLGGDGKASGTIPNLISSHGHREWEQDFRRTGMSLGG
                     GSGWVMLIYNYRDNAVHSYWAADHTNSLAWGVPLLVMDMYEHAYAIDYGANAAGYIDA
                     LF"
     gene            complement(1213..1803)
                     /locus_tag="VFY96_05170"
     CDS             complement(1213..1803)
                     /locus_tag="VFY96_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605884.1"
                     /translation="MNWNWNFDAGPPNQPPPGFSFSRTGQGRQGNWTIQNDNQAPSGA
                     YVLAQTDTDYTSYRFPVAIANEPTLRDVRVSVHCKPVSGKVDQACGVVLRYRDENNYY
                     VARANALEDNVRFYYVAGGKRHELASWSGKVVSGTWHTLSVDAKADRFEVFWNNQKVI
                     DKRDATFAEPGKVGLWTKADSVTYFDDLIVEALEGG"
     gene            complement(1882..>2175)
                     /locus_tag="VFY96_05175"
     CDS             complement(1882..>2175)
                     /locus_tag="VFY96_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEX5605885.1"
                     /translation="GEIRFDMFEAEFTHEGDKCSFEVLIERRGFIDAALKLISEMVHD
                     IDLKDSKYKREETTGIERLITGIAMAHRDDEARLQRGAAVFDDLYEYFKRKHD"
BASE COUNT          479 a          598 c          517 g          581 t
ORIGIN      
        1 gacattttgt ggggccttta tattcaagtc agatagcttt atcggccagg ctgagcgctt
       61 ctttgatgga ggcgaccgca gccgtgcaat gcacctgagc aatcgcgaga agctgttgtc
      121 cttcttcttt gctgcaaaag acctgcatta atttcgctgt tagaggtgtt ccgagactat
      181 cggacggaag ctcaacgtat ccgctctcat tctctttccc ccgtggttta aagacctctt
      241 gcagtgcatc gggactgaat ttcgataaac aatcaaaact ggattggtcg accggaatgc
      301 ataagctcat gggtttggtc cttttggggc gcatgacaga ggttcaacgg tcttttaatt
      361 catggagcga tgaaccgaaa ccggatctca agatggatca gttcaaagct gttgattttc
      421 aaccccccgc acgaggattt tcagtcctct tgccggggca ggactatatg acactatcgg
      481 tcgctatgtg tatctattca agcgttttaa tggcgcgttc tgccggtcga ttttaaaaaa
      541 gagcatcgat gtatcctgcg gcatttgcgc cataatcaat tgcgtacgcg tgctcataca
      601 tatccatgac cagcagcggt actccccagg caagactgtt ggtgtgatcg gccgcccagt
      661 aactatggac ggcattatcg cggtaattat aaattagcat cacccaaccc gagccaccgc
      721 ccagagacat tccggtacgc cgaaagtcct gctcccattc cctatgacca tgagaactga
      781 tcaaattagg gatggtgccg ctcgcttttc catcgccccc aaggttgtcg aaataaaact
      841 cgtgcagaat catggagttt gttgcgatga tctcctcctg cttcggcgac cccatttgat
      901 acggctgggc gccttttgga aggcccccga tctgttgcgg gatctgattc agtctccgaa
      961 cgacgccgct gtaattgttc tgatgatgcg agacgatgag cttttccgat aatcctttca
     1021 attttgttgg atcgaatcgc cggctgtatg tgattcatcc cgctgtacgc tggcgcggac
     1081 tgcacatttt cttgcctgtt tcctgcgcca ttgccatccc tccttcaata cgctcgggac
     1141 atcgatcaag ctggcgatca tgcatccttc tacgatgccg cgtaataatt ctcgtcgtcg
     1201 cgtcataaac aatcatcccc cttcaagcgc ttctacaatc aaatcgtcga agtaagtaac
     1261 cgaatcggcc ttggtccaaa gtcccacctt acctggttcg gcaaatgtcg cgtcacgctt
     1321 atcgatcact ttttggttgt tccagaaaac ctcaaaccga tccgccttgg catcgacgct
     1381 tagcgtgtgc cacgttccgc taacgacctt gccgctccaa cttgccagtt cgtggcgctt
     1441 tcctccggcc acgtaataaa atcgcacatt gtcttcgagg gcgtttgctc gggcgacgta
     1501 gtaattgttt tcgtctcgat accgcaaaac cacaccgcag gcctgatcga ctttgcctga
     1561 caccggcttg caatgaacgg agacgcggac atcacgaagc gtcggctcgt tggcaatggc
     1621 aacaggaaat cggtagctcg tgtagtcggt atcagtttgg gcgagaacgt aagctccgct
     1681 cggagcttgg ttatcgtttt ggatggtcca attgccttgg cggccttgtc ccgtgcggga
     1741 gaaactaaat cccggcggcg gctgattcgg cggtcccgcg tcaaaattcc agttccagtt
     1801 catagagcct cccgccgcac ccagggttaa agcgcacccg agcaacagca agattccagc
     1861 ggagaacctc ttaaccatcg ttcagtcgtg ctttcgcttg aaatactcgt acaagtcatc
     1921 aaagacggcg gctccgcgct gcagacgagc ctcgtcatct ctatgcgcca tcgcaattcc
     1981 ggtaatgagt ctttcgattc cagttgtctc ctcacgttta tattttgagt cttttaggtc
     2041 gatgtcatgg accatttccg agatcagctt aagcgccgcg tcaataaatc cccttcgttc
     2101 aattaaaact tcaaagctgc atttgtcacc ttcgtgtgtg aactcggcct cgaacatgtc
     2161 gaatctaatt tcccc
//
LOCUS       DASULZ010000251         2759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_539023, whole genome shotgun sequence.
ACCESSION   DASULZ010000251 DASULZ010000000
VERSION     DASULZ010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539023"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1104)
                     /locus_tag="VFY96_05180"
     CDS             complement(139..1104)
                     /locus_tag="VFY96_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605886.1"
                     /translation="MTTQTITLACRDYDHTRALADGRIKIDGIDVKFVNISPPSQIFL
                     RMLHDEEFDASEMSLSNYMIALGKGDRRFIAIPVFPSRVFRHAYVWINTRSGIQKPED
                     LKGKRIGIADYSMTALLFVRGFLQHQYGVMPQDVHWFRRRSEHVSIKIPTGIRIDDIG
                     KNQSLDDLLEAGDLDAVALTSPPRGFLEGLAEIKRLFADPRAVEAEYYRQTKIFPIMH
                     MVVIRRAVYEKNPGLAVRLAEAFETAKHLSFEAYAEGLSCLPWVHLDLEYARQVLGTD
                     FYPYGVSKNLATLEAATLYSNEQGLTQRKFAVNELFAPETLELFV"
     gene            1293..1454
                     /locus_tag="VFY96_05185"
     CDS             1293..1454
                     /locus_tag="VFY96_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605887.1"
                     /translation="MSLQKTFLSALLAAFLLVPAGVRGVDKVRVSGGGITPLHAIIWV
                     ANHEGLFKK"
     gene            1783..2700
                     /locus_tag="VFY96_05190"
     CDS             1783..2700
                     /locus_tag="VFY96_05190"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605888.1"
                     /translation="MRKLRVAITSLSGSMAVPWLAREAGIFKKHGLEVEVIATPSGVE
                     GMNALIAGEINFLQIAGATTVSAAVGGADVIVIGTTIGTLVQSLMVRPEIETAEQLRG
                     KSIGISRFGTTIDTGARIALRHFGLVPEKDVSIVQIGAVESIVPAMAGGRVQAGILSY
                     PAIGRARKLGFHELLDIAGLNLPYASTGITTRGEVIRKEPDLVRRYMMAQVEAIGRMK
                     RDRAFTIGVMSKFLRTNDQEQLAEAYEVYANKYLMKVPLPTVEAIRAVLEELEPRSPK
                     AKGQDPKRFFDDRFVRELQANGFIDGLYR"
BASE COUNT          647 a          685 c          812 g          615 t
ORIGIN      
        1 ggcggatcaa tccaaaaata aaccagattg atctcgatca gattatcgac tcgagtttcg
       61 ttcggagcct cgaaaacagc ggctttttgc cggatttacg caggaaaatc agctaaaccg
      121 ctaataacgc gactgcctct aaacgaagag ctccaaagtc tccggggcaa acaattcgtt
      181 tacggcaaat tttctttgag tcaggccttg ttcatttgaa tacaaggtcg cggcctctag
      241 tgtcgctaga tttttgctga caccgtacgg gtaaaaatcg gtgccgagaa cctgtcgagc
      301 atattccaga tctaaatgta cccaaggtaa acacgacaat ccttcggcat aggcttcgaa
      361 ggacaaatgc tttgcggttt caaatgcctc ggcaagacgg acagccaggc cgggattttt
      421 ttcgtacacg gcgcgccgga tcacgaccat atgcatgatt ggaaaaattt tggtctgccg
      481 atagtattcg gcttcgacgg cgcgaggatc ggcaaataat cgcttgattt ctgccaaccc
      541 ctcaagaaag ccgcgcgggg gagaagtcaa cgcaacagcg tccagatcgc ccgcctcgag
      601 caaatcgtcg aggctctgat ttttgccgat atcatcgatg cgtatgcccg tgggaatttt
      661 gatcgagacg tgctccgagc gacggcgaaa ccaatgaacg tcctgtggca tgacgccgta
      721 ttgatgctgc aagaaaccgc gcacgaagag cagcgcggtc atagaatagt cagcgatacc
      781 gattcttttg cccttcagat cctccggctt ctgaatgcct gaccgggtat tgatccagac
      841 atacgcgtgc cgaaacacgc gcgatggaaa gactggaatc gcgatgaagc gccgatcgcc
      901 tttacccagc gcaatcatgt aattggacag cgacatttcc gaggcgtcga attcttcatc
      961 gtgaagcatg cgtaaaaaga tctgcgacgg cggagagata ttgacaaatt tgacgtcgat
     1021 accgtcgatt ttgatcctgc cgtcggccag agcacgcgta tggtcgtaat cgcggcaggc
     1081 gagcgtaatc gtttgggtcg tcatgatgcc tccgtggcgg tgcgtcgaat ttaggtggaa
     1141 ctcgagcggc aagtcaatgg cacgctgtag acagtttttc gtagatgagc taaagccaat
     1201 cgctaaagga gagcgcgcgt tagacgtggc tttagtttgt aatcacatgt gcgggttatg
     1261 catccaatgc ggtcaaggcg atacgagtag ccatgagtct gcagaaaaca tttctgagtg
     1321 cgctgcttgc ggcatttctg cttgttcccg ccggagttcg gggagtggat aaggttcgcg
     1381 tcagcggcgg cggcattact ccgttgcacg caatcatttg ggtggcgaat catgaagggc
     1441 tgttcaagaa gtagggcttg gaagtggaat acctcaccat gaacagtggc acactgggag
     1501 tgcagacaaa taaaagtccg ctcatgcttc aacctgctca ctatgagggg tgtaggtggc
     1561 gactacttag ctgttcatcc tgagcaaact cgaaggacat acgccgggtt cttacaaact
     1621 ttgccgctcc cgaagacaga tttaggtgag ttatgcactg cgatacactt caagtgaaag
     1681 ggacttatga ggcaaatccg ttttgagttg atcgctttca tgtgcggctt agcttggtgc
     1741 tgtgttatcc cgcgagcata tggtgctgcg gatgcggcac cggtgcgcaa actgcgcgtt
     1801 gccatcacct cgttgtcggg atcgatggcc gtgccttggc tcgcacggga agcgggaatt
     1861 ttcaaaaagc atgggctgga agtagaagtc attgcgactc ccagcggcgt cgaggggatg
     1921 aacgctttga ttgccggcga aatcaatttt ctgcagattg ccggcgcgac gactgtgagc
     1981 gcagcggtag gcggcgccga tgtcatcgtg attggcacga cgatcgggac gctcgtgcaa
     2041 agtctgatgg ttcgcccgga aatcgaaacg gccgaacagt tgcgcggcaa atccatcggc
     2101 atcagccggt tcgggacgac catcgacacc ggggcgcgca tcgcgttgcg ccatttcgga
     2161 ctggtgcccg agaaagacgt ctcaatcgtc cagatcggcg ccgtcgagtc cattgtgccg
     2221 gcgatggcgg gagggcgcgt gcaagccggt attctgtcgt acccggcgat cggacgcgcg
     2281 cgcaagctcg gctttcacga gctgctcgat attgccggtt tgaatctccc ttacgcatcc
     2341 acgggtatta cgacacgcgg cgaggtgatt cggaaggaac cggatttggt gcgcaggtat
     2401 atgatggcgc aagtggaggc gatcgggcgc atgaagcgtg atcgggcgtt cacgattggg
     2461 gtgatgagca aattcttgcg caccaacgac caggagcaac tcgcggaagc ctacgaagtg
     2521 tatgccaaca agtacttaat gaaggtgccg ctgccgaccg tggaggcgat cagagcggtg
     2581 ctcgaagagc tcgaaccgcg aagtcccaaa gccaagggac aagatcctaa gcggtttttc
     2641 gatgaccgct tcgtgcgcga gctgcaagcc aacggtttta tcgatggact ttatcgctga
     2701 ggattcgctg catgcccgaa atgctgccaa aggtgccgag agagtcggcg cagtttgtc
//
LOCUS       DASULZ010000252         9991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1248863, whole genome shotgun sequence.
ACCESSION   DASULZ010000252 DASULZ010000000
VERSION     DASULZ010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9991
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248863"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VFY96_05195"
     CDS             complement(<1..421)
                     /locus_tag="VFY96_05195"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX5605889.1"
                     /translation="MKTLGFIGTGGMGSGMAANLIKAGNKLVVNDLRREQAKGLESQG
                     AVFKASPREVAEASEIVLSMLPYNEAVRMVGLGAGGLHEASSGAKLWIDFSSIDKKTI
                     VAVNAELSKKGWTILDGSAGGVEEAAAAGTLSLWLSGS"
     gene            complement(440..784)
                     /locus_tag="VFY96_05200"
     CDS             complement(440..784)
                     /locus_tag="VFY96_05200"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5605890.1"
                     /translation="MNEFLSFEVDDAGLDRLSWHDFGNGLSMARLAREKNAELVLYRI
                     QEDASPDAFLKHEHLGGEFYLVLKGKIADETGEYQRGDVVFLAAQSAHTPRAIGETVV
                     LVLWPAGVRILD"
     gene            complement(995..1438)
                     /gene="rplQ"
                     /locus_tag="VFY96_05205"
     CDS             complement(995..1438)
                     /gene="rplQ"
                     /locus_tag="VFY96_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX5605891.1"
                     /translation="MRHLKAGRQLSRNSAHRWALMRNLITALLREEKIQTTDPKAKEL
                     RRWVDRVITLGKRGSLHARRQALGIVQDKAVVRKLFDTIALRFKDRPGGYTRIIKLGI
                     RRGDAAHMSLIELVAAEGEKEARKTGEKKGRRARRRQRQQEEAAA"
     gene            complement(1442..2461)
                     /locus_tag="VFY96_05210"
     CDS             complement(1442..2461)
                     /locus_tag="VFY96_05210"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX5605892.1"
                     /translation="MYKHWTDLIKPKQLEVEEKTLTSNYGKFYAEPFERGFGQTIGNS
                     LRRVMLSSLMGASIVAVRIKGILHEFSTVPGVTEDVADIILNLKEIRLRLNESEQQTL
                     RIDAKGPGIVYAKDISGPPSVEILNPEHKIATLSRDAKLEMEMMVKLGRGYVPAERNK
                     EEGAPVDTIFIDAVFSPLRKVNFTVSNARVGQRTDYDRVVFEVHTDGSVKPDDAMAYA
                     AKILQDQVSIFVNFQEEPRAERKEDGPTVPLNENLFRSVDELEFSVRSQNCLQNADIK
                     YIGELVQKTEQEMLRTKNFGHKSLNEIKEILKEMGLELGMKIDHFPSREEIDARKRAA
                     EKEPV"
     gene            complement(2491..3117)
                     /gene="rpsD"
                     /locus_tag="VFY96_05215"
     CDS             complement(2491..3117)
                     /gene="rpsD"
                     /locus_tag="VFY96_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX5605893.1"
                     /translation="MARYTESVCRLCRRENLKLFLKGERCYTDKCAIERRNYPPGQHG
                     QGRQKFSEYSIQLREKQKVKRIYGLLEKQFRRTFTEAARTKGITGETLLALLERRLDN
                     VAYRLGFASSRAEARTLVRHGHVLINGRKLNIPSYTVRVGDLVSIKESSRQLGRVQTA
                     MEGAQRRGIPDWAEVDREAVSGRIRLLPSRSDITMPINEKLIVELYSK"
     gene            complement(3139..3570)
                     /gene="rpsK"
                     /locus_tag="VFY96_05220"
     CDS             complement(3139..3570)
                     /gene="rpsK"
                     /locus_tag="VFY96_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX5605894.1"
                     /translation="MADATDEKKAESPDKATERAARRKRGRKNIAEGVVHIHSTFNNT
                     IVTITDYQGNVISWSSAGAMGFKGSRKGTPFAAQQAADSAAKKAMDHGLRSVQIFVRG
                     PGAGRESALRALQSAGINISLIKDVTPIPHNGCRPPKRRRV"
     gene            complement(3583..3966)
                     /gene="rpsM"
                     /locus_tag="VFY96_05225"
     CDS             complement(3583..3966)
                     /gene="rpsM"
                     /locus_tag="VFY96_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX5605895.1"
                     /translation="MARIAGVDLPKSKRMEVALTYIYGIGRNSSRDILGKAGVSLDLK
                     SDDLSDDDTAKIRQVLEQEYKVEGDLRRDVTANIKRHLDMGSYRGQRHRRNLPVHGQR
                     THTNARTRKGPRKTVAGKKPAPSKG"
     gene            complement(4030..4143)
                     /gene="rpmJ"
                     /locus_tag="VFY96_05230"
     CDS             complement(4030..4143)
                     /gene="rpmJ"
                     /locus_tag="VFY96_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX5605896.1"
                     /translation="MKVRASVKKICNKCKVIRRKGVVRIQCENTKHKQRQG"
     gene            complement(4156..4374)
                     /gene="infA"
                     /locus_tag="VFY96_05235"
     CDS             complement(4156..4374)
                     /gene="infA"
                     /locus_tag="VFY96_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX5605897.1"
                     /translation="MPKEDAIEVTGTVLETLPNAMFRVELDNGHKVLAHISGKMRMHY
                     IKILPGDKVKIELSPYDLARGRIIFRER"
     gene            complement(4405..5163)
                     /gene="map"
                     /locus_tag="VFY96_05240"
     CDS             complement(4405..5163)
                     /gene="map"
                     /locus_tag="VFY96_05240"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX5605898.1"
                     /translation="MISLKSPREIEIMRRANAIVAEVLQELKARVAPDVTTLELDTIA
                     EQLTFKKKAVPAFKGYSVGGRVYPRCLCASINEEIVHGIPSNRALREGDIIGLDYGVI
                     YDGFYGDSAITVGVGKVSEEAQRLMDVTEQSLYRGIHELHEGKRLGDLGAAVQRFAES
                     AGFSVVRAFVGHGIGKKLHEEPPVPNYGEPDRGLRLKEGMVLAIEPMVNAGSYEVEIK
                     DDGWTAVTKDGSLAAHFEHSVAITKNGPYILSQL"
     gene            complement(5160..5816)
                     /locus_tag="VFY96_05245"
     CDS             complement(5160..5816)
                     /locus_tag="VFY96_05245"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012940719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX5605899.1"
                     /translation="MARVVLLGPPGAGKGTQAKLLLEQYGACQISTGDILRKAVAEQT
                     ALGKEAGEYIKRGALVPDDVIVNLVAERLKEPDCERGFILDGFPRTIPQAESLDRILN
                     ESGQSLNRVISVQVPKDVIIERLAGRRTCRSCGALAHVIFDPPKRNGVCDRCGGELYQ
                     REDDREATIAHRLEVYETQTAPLVDYYRQRGVLTEIDGVGNIEEIRRRIGKSLGDAAK
                     "
     gene            complement(5830..7140)
                     /gene="secY"
                     /locus_tag="VFY96_05250"
     CDS             complement(5830..7140)
                     /gene="secY"
                     /locus_tag="VFY96_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX5605900.1"
                     /translation="MLNGFQNASRIPELRRRLLFTLAMLAVYRLGIHVPTPGIDRQAM
                     AAFFDQQKNTLFGFLNLFSGGALEQFSVFSLGIMPYISASIILQLLTVVFPYLERLSK
                     EGEIGRRKITQYTRYGTVVLSIVQGMMISVGLEGTQGPGGHSLVLSAGWGFRLMTVIT
                     LTSGTAFIMWLGEQITERGIGNGISLIIFAGIVARLPSAVTTTLQFMREGELGALVAL
                     VILVLSVIVVGAIIFVERGQRRIPVQYAKRVVGRKMYGGMTSHLPLKINTANVIPPIF
                     ASSILIFPATLAQFANHPWVQTISGWLSPGNWLHDLIYVILIIFFAFFYTAVVFNPVD
                     VADNMKKAGGYIPGIRPGQKTAEYIERVLSRITLSGAIYLCVVCLLPTILVRQFNVPF
                     YFGGTALLIVVGVALDTVAQAETHLITRNYEGFMKRGRLRGRRG"
     gene            complement(7133..7582)
                     /gene="rplO"
                     /locus_tag="VFY96_05255"
     CDS             complement(7133..7582)
                     /gene="rplO"
                     /locus_tag="VFY96_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX5605901.1"
                     /translation="MRVDELKPAPGSNKKRKRVGRGDGSGHGKTSGRGHKGQGARSGG
                     NVQPGFEGGQMPLQRRLPKRGFHNAFRVQMSVVNLSQLESLSENEITPESLAQHGLIS
                     GKGRRVKVLGDGALTRALSVKAHGFSAAAKEKIEAAGGKVELIPVHA"
     gene            complement(7585..8082)
                     /gene="rpsE"
                     /locus_tag="VFY96_05260"
     CDS             complement(7585..8082)
                     /gene="rpsE"
                     /locus_tag="VFY96_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX5605902.1"
                     /translation="MERIDPQGLELKEKVVHISRVAKVVKGGRRFSFSALVVVGDNRS
                     VVGYGMGKANEVPEAIRKGLEQAKKNLIRVPVMDGTIPFEVTGHYGAGYVILKPASDG
                     TGVIAGGGVRAVVEASGIQNVLTKCLGSNNPHNMVKATIEALRDLRAPEEIADRRGKI
                     LAEIR"
     gene            complement(8109..8462)
                     /gene="rplR"
                     /locus_tag="VFY96_05265"
     CDS             complement(8109..8462)
                     /gene="rplR"
                     /locus_tag="VFY96_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX5605903.1"
                     /translation="MANKRGNIARLRRKMRVRQKVQGTEARPRVCVFRSNKHIYAQVI
                     SDSEGKTLAAASSLAATEKAPKGVEAAKQVGLALAKICKEKNINRVIFDRNGFLFHGQ
                     VKALADGAREGGLEF"
     gene            complement(8477..9028)
                     /gene="rplF"
                     /locus_tag="VFY96_05270"
     CDS             complement(8477..9028)
                     /gene="rplF"
                     /locus_tag="VFY96_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020520467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX5605904.1"
                     /translation="MSRIGKLPVTIPSGVKVAIDSNGVRLEGPKGKLQTILPPGVDVK
                     VEGNVVRIERRNDERKSRAVHGLTRKLIANMAQGVSQGFTRVLDINGVGYRAEVKGQE
                     IYMTLGYSHPVVFPLPAGISASVERQIIITLTSADRQLLGETAAKIRSLRPPEPYKGK
                     GIKYREEVIKRKAGKAVGSAGGK"
     gene            complement(9047..9451)
                     /gene="rpsH"
                     /locus_tag="VFY96_05275"
     CDS             complement(9047..9451)
                     /gene="rpsH"
                     /locus_tag="VFY96_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX5605905.1"
                     /translation="MGMTDPIADMLTRIRNASRARHQRVSVPWSRLKESIIKILIEEG
                     YLKDQKKVKAAIGGGEEIVIQLKFDRENRPIIGGLKRVSTPGRRVYVSSKSMAPVRKG
                     LGVHVLSTPKGILVDREAQKAKVGGELICSVW"
     gene            complement(9466..9651)
                     /locus_tag="VFY96_05280"
     CDS             complement(9466..9651)
                     /locus_tag="VFY96_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEX5605906.1"
                     /translation="MAKTCLRIKAERPNKFKVREYNRCPLCGRARAFYRRFRMCRLCL
                     RDHARKGHIPGLIKASW"
     gene            complement(9666..>9991)
                     /gene="rplE"
                     /locus_tag="VFY96_05285"
     CDS             complement(9666..>9991)
                     /gene="rplE"
                     /locus_tag="VFY96_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011434164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX5605907.1"
                     /translation="AIANFKLREGVPIGCMVTLRRERMYEFLDRLIHVALPRVRDFKG
                     VSDRAFDGRGNYSLGLREQIIFPEIHADRVEKTRGMTITIATTAKTDQEGRTLLRLMG
                     MPFAS"
BASE COUNT         2082 a         3053 c         2466 g         2390 t
ORIGIN      
        1 tggacccgga aagccaaagc gacaatgtcc cggccgcggc ggcttcctcg accccacccg
       61 cggagccatc taaaatcgtc cagccttttt tcgacaactc ggcattcact gcgacaattg
      121 ttttcttgtc gatgctgctg aaatcgatcc acagtttggc gccgctcgag gcttcgtgta
      181 acccgcctgc gcctagacca accatgcgca ctgcctcatt gtaaggcagc atcgataaaa
      241 caatttcgga tgcctcggcc acctctcggg gagacgcctt gaacaccgcg ccctggctct
      301 ccagcccttt ggcctgctca cggcgcaaat cattgacgac cagtttgttt ccggccttga
      361 tcaagtttgc cgccatgccg ctacccatgc cgccggttcc aatgaatccc aaagtcttca
      421 ttttctcctc ctgagcagct caatcgagaa tgcgcactcc cgccggccac aacaccaaga
      481 caaccgtttc acctatggct ctcggtgtat gcgccgattg cgcggccaag aaaaccacat
      541 ctcccctttg atactcaccg gtttcgtcgg caattttacc cttcagtacc aagtagaatt
      601 cccccccgag atgttcatgc ttgaggaatg cgtccggcga ggcgtcctcc tggattcggt
      661 aaagcaccag ctcagcattc ttttcgcggg cgagacgcgc catcgagagg ccatttccga
      721 aatcgtgcca tgaaagtcgg tccaaccccg catcatcgac ctcgaagctg aggaattcgt
      781 tcaaggattg gcgcaatacg tgccccgttc ggtgatccac gagcgattaa ttatcatgag
      841 gcgacagcga acaaaagcat gctcccgcgc attttcgacg ttcccgcact agttttaacg
      901 atccggacca gagctgtttt ccggtcagca ataaaaaaga aaggcccgcg gagtaaaatt
      961 tctcgcgggc cttttattgg gttaggaata ggtgctaagc cgccgcttct tcctgctgtc
     1021 tttgccgccg gcgcgctctt cgacccttct tttcgccagt tttccgagct tccttttcgc
     1081 cttccgcagc gaccagctcg atcaatgaca tgtgcgcggc atcgccgcgg cggattccga
     1141 gcttgatgat ccgcgtatac ccgccgggcc ggtctttgaa acgcagcgca atcgtgtcga
     1201 aaagttttcg caccaccgct ttatcctgaa caatgccgag cgcttgacgg cgtgcatgca
     1261 agctgccgcg tttgccaagc gtaatcacgc gatcaaccca acggcgcagc tccttcgctt
     1321 tcggatcggt ggtctggatc ttttcctccc gcagcagcgc ggtgatcaga tttcgcatga
     1381 gcgcccaccg atgcgccgaa ttgcggctca gctgtcttcc ggccttgaga tgacgcatgg
     1441 tttataccgg ctccttttca gccgcgcgtt tacgcgcgtc gatttcctct cgggacggaa
     1501 aatggtcgat tttcatgcct agttcgaggc ccatttcctt gagaatctct ttgatttcgt
     1561 tgagcgattt gtggccgaag tttttcgtcc ggagcatttc ctgctcagtt ttttgcacca
     1621 gctcgccgat gtacttgata tcggcatttt gcaggcaatt ttgcgagcgc acggaaaact
     1681 cgagctcgtc gacactgcga aacagatttt cgttgagtgg aacggtcggt ccgtcttcct
     1741 tccgttccgc gcgcggttct tcctggaaat taacgaagat gctcacttgg tcctggagaa
     1801 tcttcgcggc ataggccatc gcatcatccg gtttcacgct gccatccgta tgaacttcaa
     1861 atacgacgcg gtcgtagtcg gtcctttgac cgacgcgcgc attggacacg gtgaaattca
     1921 cttttcggag cggcgagaac acggcatcga tgaagatcgt gtcaaccggg gcgccctcct
     1981 ctttgttgcg ttccgcaggc acatagccgc gaccgagttt gaccatcatt tccatttcga
     2041 gcttagcgtc gcgcgacagt gtagcgattt tgtgctcggg attcagaatt tcgacgctcg
     2101 gcggaccgct gatgtctttg gcgtaaacaa tcccgggtcc tttggcatcg atgcgcaacg
     2161 tctgttgctc gctttcattg agccggagcc ggatctcttt taaattaagg ataatatcgg
     2221 cgacatcttc ggtcacaccc ggcactgtcg agaactcgtg caagatccct ttgatccgca
     2281 ctgcgacaat cgacgcgccc atgagcgatg aaagcataac ccggcgcaat gaattgccga
     2341 tcgtctggcc gaagccccgc tcgaacggct ccgcgtaaaa cttaccgtag tttgaggtca
     2401 gagtcttctc ttccacctcc aattgcttag gcttaatcaa atccgtccaa tgcttgtaca
     2461 ttgtagacat agcgtctccc cggctcgaac tcacttcgag tacaactcga caatcagttt
     2521 ttcgttgatc ggcatcgtga tatcgctgcg cgatggcaat aacctaatcc ggccgcttac
     2581 ggcttcgcgg tccacttccg cccaatccgg aataccgcgc cgctgtgctc cctccatggc
     2641 ggtctgcacc cggccgagtt gccggctcga ttctttgatc gagactaggt ctccaacccg
     2701 tacggtgtaa gatgggatat tgagcttcct gccattgata agcacgtggc cgtgtcgaac
     2761 cagggttcgc gcctcggcgc gtgagctggc gaagccgagt cgataggcga cattgtcgag
     2821 gcgacgttcc agcaacgcga gcagcgtctc gccggtaatg cctttggtcc gcgcagcctc
     2881 ggtgaaggta cggcgaaact gcttttcgag caaaccgtaa atacgcttca ccttctgctt
     2941 ttcgcgcagc tgaatactgt attcggaaaa tttctgtcta ccttggccat gctgtcccgg
     3001 cggataattc cgtcgttcga tggcgcactt atccgtgtaa caacgctccc cttttaaaaa
     3061 aagcttcaaa ttttcgcgcc tgcaaagtcg gcaaaccgat tcggtatatc tagccaagcc
     3121 aatctcctcc tgtacctatt agacccgccg ccgcttcggc ggacggcagc cgttatgcgg
     3181 gatcggcgtc acatctttga tcaaactaat attgatgcct gccgattgca gcgcgcgcag
     3241 tgccgattcc cgccccgctc ctggaccccg gacgaagatc tgcacgctcc tcaggccgtg
     3301 atccatggct ttttttgccg cgctgtccgc cgcctgctgt gcggcgaagg gcgtaccctt
     3361 acgcgatcct ttaaatccca tcgcgcccgc gctcgaccac gaaatgacgt ttccctggta
     3421 atccgtaatc gtgacgatgg tattgttaaa ggtcgaatgg atatgcacca ctccttcggc
     3481 gatatttttt cgcccgcgct ttcgccgcgc agcgcgctcg gtagccttat ccggcgactc
     3541 ggcctttttc tcatccgtgg catctgccat actcgagtcc tcttagcctt tcgacggcgc
     3601 cggcttcttg cccgcgacgg ttttgcgcgg tcctttgcga gtacgggcgt tggtatgcgt
     3661 gcgctgcccg tgcaccggca gatttctgcg atgacgctgc ccgcgatacg atcccatgtc
     3721 gaggtgccgc ttgatgtttg cggtgacgtc gcgcctgaga tcgccctcga ccttgtactc
     3781 ctgttcgagc acctggcgga tcttggccgt gtcgtcatcg ctcaaatcat cactttttaa
     3841 atcaagcgaa acgcctgcct tccccaatat gtcccgtgat gagttgcgcc caattccata
     3901 aatataggtg agcgcaactt ccattcgctt gctcttgggc aaatctacgc ctgcaatgcg
     3961 tgccatagtc tcactccatt cgtgacaagt tcagaagaga acgtaaactt tgaccgaaaa
     4021 cctaaacggt tatccctgcc gttgcttgtg tttggtgttc tcgcactgga tgcgcacaac
     4081 gcccttacgg cgaatgactt tgcacttgtt gcaaatcttt tttaccgatg cccgaacctt
     4141 cacggccgct cctctctaac gttcccgaaa aataatcctt ccgcgggcca gatcgtatgg
     4201 cgagagttcg atcttcactt tgtcaccggg aagaattttg atgtagtgca tgcgcatctt
     4261 tccggaaata tgcgcgagca ccttgtgccc attgtccagc tccacgcgaa acatcgcgtt
     4321 cggcagcgtc tctagcaccg tgccggttac ttcaatggcg tcttcttttg gcatagacaa
     4381 cctgcctgtc gcggcccgcc gcgattagag ctgactcaga atatagggcc cgtttttagt
     4441 gatcgccacc gaatgctcga aatgggcggc caagctgcca tctttagtaa ccgcggtcca
     4501 accgtcgtct ttgatttcaa cttcgtaact cccggcattg accataggtt cgatcgccag
     4561 gaccattccc tctttcaacc gcaaaccgcg atccggctca ccgtaattag gcaccggcgg
     4621 ttcctcgtgc agcttcttac cgatcccgtg accgacaaac gcacgcacca cagaaaaccc
     4681 ggcgctttcg gcaaaccgct gcacagccgc gccgagatca cccaatcgct tgccttcgtg
     4741 aagttcgtga atgcctcgat agagcgattg ttcggtaacg tccatcaacc gctgcgcctc
     4801 ttcgctcacc tttccgacgc ccacggtaat cgcggagtcg ccgtaaaaac catcataaat
     4861 cacgccatag tctaaaccga taatgtcccc ctcgcgaagc gcccggttcg acggaatgcc
     4921 atgcacgatc tcctcgttga tggaagcgca caaacaacgc ggataaacgc gacccccgac
     4981 actatagccc ttgaatgccg gaaccgcttt cttcttaaac gtcagctgtt ccgcgatggt
     5041 gtccagctcc aaggtggtca catcaggagc aacccgcgct ttcagctcct gcaagacttc
     5101 cgcgacgatc gcattcgccc gcctcatgat ttcgatttct cgaggcgatt tgagcgaaat
     5161 cacttcgctg catcccccaa cgatttgccg atccggcggc ggatttcttc gatgtttccg
     5221 actccatcaa tttccgtgag cacaccgcgc tgacggtaat aatcgaccag cggcgccgtt
     5281 tgggtctcat aaacctcaag ccggtgcgca atggtcgctt cgcggtcgtc ctcgcgttgg
     5341 taaagctcgc cgccgcagcg gtcacagaca ccgtttcgct ttggcggatc aaaaatgaca
     5401 tgggccaacg ccccacagct tcggcaggtg cgccgtcctg caaggcgctc gataatcacg
     5461 tccttcggca cttgaaccga gatgacccga tttaaactct gcccgctttc gttcaggatc
     5521 cgatcgaggc tctcggcctg tggaatcgtc cgcgggaaac catcgagaat aaacccgcgc
     5581 tcacagtccg gttctttcag ccgctccgcg actagattca cgatgacgtc atctggcacg
     5641 agcgcgccac gtttaatata ttcgccagct tctttgccca aagcggtctg ctccgcaacg
     5701 gctttgcgca aaatatcacc agtcgagatt tgacaggcgc cgtattgttc taggagcaac
     5761 tttgcctggg ttcccttacc tgctcccggc ggacccaata aaacaactcg cgccactgcc
     5821 gccactactt tagccccttc ttccgcgcag ccgtccccgc ttcataaatc cttcatagtt
     5881 gcgggtgatc aagtgagttt ccgcctgggc gaccgtatcc agggccacgc ccaccacgat
     5941 caaaagcgcg gtgccgccga aatagaacgg gacgttaaac tgccggacca atatggtcgg
     6001 caaaagacaa acgacgcaca gatagatcgc tccgctcaag gtgatgcgcg acagcacccg
     6061 ttcgatgtac tcggctgttt tttgcccggg ccgaatgccg ggaatataac cgcccgcctt
     6121 cttcatgtta tcggcgacgt cgaccggatt aaaaacgact gcggtgtaaa agaacgcgaa
     6181 aaagatgatc aggatgacgt agatcaggtc gtgcagccag ttccccggtg agagccaccc
     6241 ggagatggtc tgcacccacg gatgattggc aaattgggcc aaggtcgcgg gaaagatcag
     6301 gatcgaggat gcgaagatcg gcggtatgac attagccgtg ttaatcttga gcggcagatg
     6361 ggaagtcatc ccaccataca tctttcttcc cacaacgcgt ttggcatact gtaccgggat
     6421 ccggcgctgt ccgcgctcga cgaaaatgat cgcacccacc acaatcacgc tgagcacgag
     6481 gatcaccaaa gccaccaagg cgcccaattc accctcgcgc atgaactgca aagtcgtcgt
     6541 gaccgccgat ggcaatcggg cgacgatacc ggcaaaaatg atcagtgata taccattacc
     6601 aataccgcgc tcggtaatct gctcgcctag ccacatgata aatgcggtcc ccgaggtaag
     6661 cgtgatcacc gtcattagac gaaaacccca tccggctgac aacaccaagc tgtgtccgcc
     6721 aggcccttgg gtgccctcga gcccaacact gatcatcatg ccttgaacga ttgacaagac
     6781 gacggtgccg tagcgcgtgt actgagtaat tttgcggcgg ccgatttccc cttcctttga
     6841 gagtctctcc agataaggga aaacgaccgt cagcagctgt aagataatcg atgcgctgat
     6901 atagggcatg atgcccaatg aaaaaaccga aaattgctcg agcgcgccgc ctgaaaataa
     6961 attgagaaag ccgaaaaggg tattcttttg ctgatcaaaa aatgccgcca tcgcctgacg
     7021 atcgataccc ggcgttggca catgaatacc caggcgatag accgccagca tggccagcgt
     7081 aaaaagcaga cgccggcgca actcgggaat tcgcgaagcg ttctgaaatc cgttaagcat
     7141 ggacaggtat taactccact ttaccgcccg ccgcttcgat cttttctttg gcggcggcgg
     7201 aaaatccatg agctttgacc gataaggctc tagtcaatgc cccatcgccg agaactttta
     7261 cccggcgacc cttgcctgag attaaaccat gctgggccaa gctttcgggg gttatttcat
     7321 tctcggagag agactcaagc tgactaagat taacgaccga catttggact cggaaagcgt
     7381 tatggaagcc ccgcttgggt aaacgcctct gcaaaggcat ttgtccgcct tcgaagccag
     7441 gttggacgtt cccgcctgaa cgtgcgcctt gtcctttatg accccggcct gaagttttgc
     7501 cgtgccctga accatcgccg cggccgactc gcttgcgctt tttattcgac ccgggtgctg
     7561 gcttgagttc gtcgactctc atagttaacg aatctccgct agaattttgc cccgccggtc
     7621 ggcgatctct tcaggagcgc gcaagtcccg caatgcttct atggtcgctt tcaccatgtt
     7681 gtgtggattg ttggagccaa ggcatttggt taaaacgttc tgaattcccg aagcctcgac
     7741 tacggcgcga acgccgccgc ccgcaattac gccggtccca tccgaggccg gtttaaggat
     7801 gacatatcct gcgccgtaat gacccgtgac ttcaaaaggt atcgtgccgt ccatgaccgg
     7861 cacccgtatt aagttctttt tggcttgctc cagaccctta cggattgctt ccggcacttc
     7921 gttcgcttta cccatgccgt aaccgacaac cgagcggttg tcgccgacaa cgacaagagc
     7981 gctaaagctg aagcgccgtc caccttttac gactttcgcc actcggctga tgtgaacgac
     8041 tttttctttt aattccaaac cctgcggatc aatccgttcc aaaggcacct ctcgtctttc
     8101 aggctgtttc agaattccaa gccgccctcg cgagcaccgt cggccagagc cttgacttgg
     8161 ccgtggaaaa gaaaaccatt acgatcgaag atcactctgt tgatattttt ttctttacag
     8221 atcttggcca acgcaagccc aacctgtttg gcggcttcca cgcccttggg cgccttttcc
     8281 gtcgcagcaa gactcgaggc tgcagccagg gttttccctt cactatcaga gattacctga
     8341 gcataaatgt gtttattgct acggaagaca cagacccgcg gccgcgcctc cgtgccctgg
     8401 actttttggc ggactcgcat ttttcggcgc agccgcgcga tattgcctcg tttattggcc
     8461 atctttcatc cccaaactac tttccgccag ccgaccctac ggctttcccc gctttgcgtt
     8521 tgattacttc ctcgcgatac ttgatgccct tgcctttata gggctcgggc ggtctgaggc
     8581 tgcgaatttt tgccgcggtt tcccccagca actgacgatc ggcgctagtc aatgtaatga
     8641 taatctgtcg ttccaccgac gcgcttatac ccgctggtaa cgggaacacc accggatgag
     8701 aatagcccag ggtcatgtag atttcctgac ctttgacttc ggctcggtaa ccgacgccgt
     8761 tgatatcgag cactcgggtg aatccttggc ttaccccttg cgccatgttc gcgatcagct
     8821 ttcgcgtaag tccgtgtacg gctctcgatt tgcgttcatc gtttcgccgc tcgatgcgca
     8881 ccacgttgcc ctcgactttg acatcaacac ccggcggcaa aatggtttgc agcttacctt
     8941 tcggtccttc caagcgaacg ccattcgaat cgatcgcgac cttcacacca ctcggaatcg
     9001 ttaccggcaa ttttccaatc cgcgacactg gaacacctcg cttaaactac cataccgagc
     9061 aaatcagttc gccaccgacc ttggccttct gggcttcacg atccaccaga atccctttcg
     9121 gggtcgagag cacatgcacc cccaagcctt tgcgcacagg agccatcgac ttggagctga
     9181 cataaactcg gcgcccgggc gtactgactc tttttaatcc cccgatgatc gggcgattct
     9241 cgcgatcgaa cttgagctga atcacgatct cctcaccccc accgatcgcg gctttgacct
     9301 ttttttgatc cttcaagtag ccttcttcga tcaggatctt gataatactc tccttcaatc
     9361 gtgaccacgg caccgaaacc ctttgatggc gcgcccgtga cgcattccga atgcgcgtta
     9421 gcatatctgc gattggatcg gtcattccca tctttcttgc tccaattacc agctggcctt
     9481 gatcaggcct gggatatggc ccttgcgagc gtgatcgcgg aggcaaagcc ggcacatgcg
     9541 aaagcgccgg taaaaagccc gggcgcggcc acaaagtgga cagcggttat actcccgcac
     9601 tttgaacttg ttcggcctct cggccttgat tcgaagacat gtcttggcca atggattctc
     9661 tccctctaac tcgcgaacgg cattcccatc aatctgagca gggttcgtcc ttcctgatct
     9721 gtttttgctg tggtcgcgat cgtgatcgtc atgccgcgcg ttttttcgac cctgtcagca
     9781 tgaatttccg ggaaaataat ttgctcccgt aaacccaggg aataattgcc gcgaccgtcg
     9841 aaggcccggt ccgacacccc tttgaagtcg cgcacacgcg gcagcgccac atgaatcaag
     9901 cgatccagga actcatacat gcgctcgcgg cggagcgtca ccatgcagcc gatcggaacg
     9961 ccttcgcgga gcttgaagtt ggcgatggct t
//
LOCUS       DASULZ010000253         3424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_741286, whole genome shotgun sequence.
ACCESSION   DASULZ010000253 DASULZ010000000
VERSION     DASULZ010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741286"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VFY96_05290"
     CDS             <1..589
                     /locus_tag="VFY96_05290"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5605908.1"
                     /translation="VIGEPIKPFVVLVCGLTGSGKSTISRELARRLDVPLINSDVVRK
                     QIASKAGPQIVPFNEGIYSPAMTEKTYATMVSEAEEELAKGQGLILDATFSRRAQRKA
                     IIDLANRYGARVAVIHCVASPTTIEQRLKKRAAAGSDVSDGRWEIYLAQKAAAEPLDD
                     IPPSLQLELQTDASVEELGNACEEFLRSRLAHMHK"
     gene            complement(714..1139)
                     /locus_tag="VFY96_05295"
     CDS             complement(714..1139)
                     /locus_tag="VFY96_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HEX5605909.1"
                     /translation="MPRFPVSSEKERQLVERMAALEIREEDIEEQFVRSSGAGGQNVN
                     KVSSCVLLHHRPSGVRVRCQKERSQGLNRFLARRILLEKIESLRRGAAVEAQQRIAKI
                     RRQKRRRSRRAKLRLIEEKRRRGETKTLRSAVRPDTDDS"
     gene            complement(1147..2355)
                     /gene="ilvA"
                     /locus_tag="VFY96_05300"
     CDS             complement(1147..2355)
                     /gene="ilvA"
                     /locus_tag="VFY96_05300"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEX5605910.1"
                     /translation="MISFVDIEEARERIREQIYLSPFPHSETISQMTGNRIFFKLENL
                     QLTGSFKERGALNRLLMLSGEEAHQGVIAASAGNHGMAVAYHSRRLHLKATIVMPISA
                     PLIKVTRVRQYGAETLLHGNDYDAAYTEAVRLSRASGPCFISAFDDLWVIAGQGTIGI
                     ELYEQNPDLEVAVVPVGGGGLIAGIALALKTLLPKIRIIGVQAEAVPSMKVALDQGAP
                     AQLGPATTIADGIAVRAVGALPFELVRRYVDEIVTVNEREIANAVLLLLEIEKTVAEG
                     AAAVPLAALVNKKIAVTGKNVGLIVTGGNIDMNLISRIIETGLVQDGRLSRFAIVISD
                     RPGSLARLAQRVADLGANILQITHSRSFGQMAIGETEVELILETAGPEHIERIYSALT
                     ADKFKIKSAG"
     gene            2497..>3424
                     /locus_tag="VFY96_05305"
     CDS             2497..>3424
                     /locus_tag="VFY96_05305"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEX5605911.1"
                     /translation="MIRAGVGQSQEKVTRCAVEKAAAQALSRAGISRADFAIIFFTAD
                     HAAQSSDWLTGLSQAVGTDCVVGSSGAGILTGEGEIEGGPGVVVCVVASDTLVARPFI
                     FEPLRGNETNLGVSFGDFLAKAGEKNSLMILLPDSYQGNPQPLLAGMARRAGFHPVIG
                     AGSSENGTTGATYQLCGDKLATNSVAGAYLSGDFTLQVDITQGCQPITERMVITKAER
                     NLIYEIDGRPALEIFARLLKGPLAEDVRRAVSVIFVGLPANRQENSVADGQYVVRNII
                     GMDAEKGIIGVADEVTEGDAVIFTMRDGQRARE"
BASE COUNT          728 a          960 c          993 g          743 t
ORIGIN      
        1 agtgataggg gagccgatca agccattcgt cgttctcgtc tgcgggttga ccggcagcgg
       61 caaatcgacg atttcccgag agcttgcccg cagattagat gtgccactga tcaattccga
      121 tgtagtccgc aaacagatcg cttcgaaggc gggcccgcaa attgttccat tcaatgaggg
      181 tatctacagt ccggcaatga cggaaaaaac ctatgcaaca atggtgtctg aagccgaaga
      241 agaacttgct aaagggcagg ggctgattct cgatgccacg ttcagccggc gcgcgcaaag
      301 aaaagcgatt atcgacctcg ccaaccgcta cggcgcgcgg gttgcggtca ttcactgtgt
      361 ggcgtccccc acaacaatcg agcaaagact aaaaaagcgc gcggcggcag gatccgacgt
      421 ttccgacggt aggtgggaaa tctatctggc ccagaaggct gcggccgagc cgcttgacga
      481 tattccgccg agtctgcagc tggagctcca aaccgatgcc tccgtcgaag agctgggcaa
      541 tgcgtgcgag gagtttctcc gttcgcgctt ggctcacatg cacaagtaaa atagtcttcg
      601 gatcgaaaat tgcctcgccc attccgatgg gagaaggttg gggtgagggg tggcttgggc
      661 taacataaaa ctatgaacat tctactaaac tcacctcgtc aatcactatc aatctaacta
      721 tcgtccgtat cgggtcggac ggcgctccgg agcgttttgg tttctccgcg gcggcgcttt
      781 tcctcgatca gccgaagttt agcgcggcgc gagcggcggc gtttctgccg gcgaattttc
      841 gcgatgcgtt gctgcgcttc gacggcagca cctcggcgca aggattcgat cttttccagg
      901 agaatccttc gtgccaggaa acgattcaag ccttgggatc gctccttctg acatcgcacc
      961 cgaacaccgc tgggacgatg gtgcagtagg acacagctcg aaaccttgtt gacattctgc
     1021 ccgccggcgc cggaggagcg cacgaactgc tcctcgatgt cttcttcgcg tatctcgaga
     1081 gctgccatgc gctcgaccaa ttgtcgttcc ttttcggagg aaaccggaaa acgaggcatc
     1141 gaaagttcaa cctgcgcttt tgattttaaa tttgtccgcg gtcaacgccg aataaattcg
     1201 ctcgatatgc tccgggccgg cagtttccag gatcagttcg acctcggtct ctccgatcgc
     1261 catttgcccg aagctgcggc tgtgggtgat ctgcaagata ttcgcaccca aatccgcgac
     1321 ccgctgtgca agccgcgcca aacttcccgg ccggtccgaa atgactatgg caaatctgct
     1381 caaccggccg tcttgaacca gaccggtctc gataatccgt gaaattaggt tcatgtcgat
     1441 gttgccgccc gttacaatca gtccgacgtt tttgccggtg acggcaattt tcttattgac
     1501 cagcgctgcc agcggcacag ccgccgctcc ttcggctacg gtcttctcaa tctcgagcag
     1561 cagcagcaca gcgttggcga tctcgcgctc gttcacggtg acgatctcgt cgacataacg
     1621 tcggaccaat tcgaacggca gcgctcccac cgcgcgcacg gcgatgccat cggcgatggt
     1681 ggttgcgggc ccgagctgcg ccggagcgcc ttgatcgaga gcgactttca tcgaggggac
     1741 cgcttcagcc tggacaccga tgatgcggat ttttggcagc agagttttga gcgctagcgc
     1801 gatgcccgcg attaacccgc cgccgccgac cggcacgacc gcgacttcca gatcaggatt
     1861 ttgctcgtaa agctcgatcc cgatcgtgcc ctggccggcg atgacccata aatcgtcgaa
     1921 tgccgaaatg aagcatggcc ccgaggcgcg gctcaagcgc actgcttccg tgtaggcggc
     1981 atcataatca ttcccgtgca gcagtgtttc ggcaccgtac tgtcgcacgc gggtgacttt
     2041 gatcagtggt gccgagatcg gcataacgat tgttgccttg agatggagac gccggctgtg
     2101 atacgcgacc gccatgccgt ggtttccggc gctagccgcg atcacacctt ggtgggcttc
     2161 ttcaccgctc agcatgagca aacgattgag cgcgccgcgc tctttgaagg atcctgtcag
     2221 ttgtaaattt tccagtttga agaaaattcg attaccggtc atctggctga tggtctcgga
     2281 atgcggaaaa ggcgagaggt agatttgctc ccgaatgcgc tcgcgggctt cctcgatatc
     2341 gacgaaggaa atcatgctag caaagtagaa tgaaaagatg tacgcgcgca accaatctcg
     2401 acctctcgtc acgccacctc acatcaacga tgacgccatt aaataaagcg attcggcttg
     2461 cttccgcgac agcgaatttt ctatagtaaa gcaatcatga ttcgagccgg agttggacaa
     2521 tctcaagaaa aagtaaccag atgtgcggtc gagaaggctg ccgctcaagc attgtcgcgt
     2581 gcggggatct cgcgagcgga tttcgcgatc attttcttca ctgcggatca cgcggcacag
     2641 tcgagcgatt ggcttacagg gcttagtcaa gccgtcggga cggattgcgt cgtgggttcg
     2701 agcggcgccg gcattttgac tggcgaaggt gagattgaag gtgggcctgg agtcgtcgtt
     2761 tgcgtggttg cctcggatac gctcgttgct cgaccgttta tattcgagcc gttgcgcggc
     2821 aacgaaacga acttgggtgt cagctttggt gattttctcg cgaaggccgg agagaaaaat
     2881 tcattgatga ttttgctgcc tgactcgtac cagggaaatc cacaaccgct gctcgccgga
     2941 atggcccggc gcgccggttt tcatccggtg attggcgccg gttcgagtga gaacggcacc
     3001 accggtgcga cctatcagct ctgtggtgac aagctcgcca caaactccgt ggctggagcc
     3061 tatctctccg gcgatttcac cctgcaggtc gatatcaccc agggctgcca gccgattaca
     3121 gaacggatgg tgattaccaa ggccgaacga aacctcattt acgagatcga tgggcgaccg
     3181 gcgctcgaaa tttttgctcg cctgctgaag ggcccgctcg ccgaggacgt cagacgcgcg
     3241 gtgagcgtca tcttcgtcgg gctccccgcg aatcgccagg aaaacagcgt cgccgacgga
     3301 cagtacgtcg tccggaacat catcggcatg gatgccgaga agggtattat cggcgtcgcg
     3361 gacgaagtga ctgaaggtga cgcggtgatt tttaccatgc gggacggtca gcgggcgcgc
     3421 gaag
//
LOCUS       DASULZ010000254         3062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1451590, whole genome shotgun sequence.
ACCESSION   DASULZ010000254 DASULZ010000000
VERSION     DASULZ010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451590"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..1343
                     /locus_tag="VFY96_05310"
     CDS             81..1343
                     /locus_tag="VFY96_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605912.1"
                     /translation="MSEEATNKFVAALKTGAGLKNEFIYYMSFDEYKTCPDPFPRTKF
                     TVQEVQESIRRYPHPQKRDELFQWDIHGRLFRPAKVSIPNTAVVMVHGGAANEYEFIF
                     TPDGPEEYPDLTQTDPAKARVGVAQHIASLGVPVLAISLPGHYSRKAWPPIADRRPEF
                     VIDEIPSAEELKNRLAVYTFRMCVEAIKKLVEKTLEESIFMWGHSTGGEYFYLMEQFG
                     LKNKLIGGLGFGTGMPAWVRKEWDLACARKSPEERAAPFRNLTDLSRRSPAGYVKSGY
                     IGPNQPWGSAERWFELENHRRPQFKPFLQDIEHSAHDVLLPDIKKISGLADEDLFITY
                     KSDLHRLRGKKLLHIVGEYDKGHWIEGGERGLQFRREVYAFHRFKPYADALRLIVVPN
                     LTHYGHVESYNERLANLMVTGFKEYFCG"
     gene            1414..2436
                     /locus_tag="VFY96_05315"
     CDS             1414..2436
                     /locus_tag="VFY96_05315"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605913.1"
                     /translation="MKSSTQRARATFRRMLSHSVAIVVLFSAVCVAAEPLRIAYTSIA
                     VVYGPLWLTKEAGIFKKYNIEPEFIYIAGGPPSLQALIAGDVAISFTAAGATVAANLQ
                     GSDVVLLGATIDSLPFELWSIPTIKQPEQLRGTKLGVSRIGATTDFIARYLLKKWNLQ
                     PDKEVAIFQTGSGPQVFAALKGGAVQSGVLSTGPETLNAEAAGFFRLADVSSSGLVYP
                     FGPFAARQAFLKTHAALAMRFMKAYVEGIHRFKTDKAAALSVLDKYTKQKATPAAEKI
                     YELYATKYFKRAPEATSAAIQTILEELSATRTLPAGITPQRFTESRFIGELIRDGFVD
                     SLYKNR"
     gene            2603..>3062
                     /locus_tag="VFY96_05320"
     CDS             2603..>3062
                     /locus_tag="VFY96_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605914.1"
                     /translation="MPYGILDPERLSKASRISIERAIDWWQKSMLTSKHKTYFVLAGY
                     DSDQGQEIKLRRAIIEETVHEPELLANLIEVSARNETALAQKIARVRALLPVETITVF
                     VESRNAVSVKAIFKRKFGKTLQIRKFKATFEFNHQWITTSTSFAWFSRNWF"
BASE COUNT          731 a          867 c          806 g          658 t
ORIGIN      
        1 tcatccgcgg tcgttcaatt tgacaatact cacagttaac ccgcatataa ttagccagac
       61 gcaaaatcca ggagaccgca atgagtgaag aagcaacgaa caaattcgtc gcggcgctaa
      121 aaaccggcgc cggactgaag aatgaattta tctactacat gtctttcgac gagtataaaa
      181 cgtgtcccga tccatttccc cgcaccaaat tcacggttca agaagttcaa gaaagcatcc
      241 gcagatatcc acatccgcaa aagcgtgacg aactttttca gtgggatatt catggtcgat
      301 tatttaggcc tgctaaagtt tccattccca atacggcagt ggtcatggtt cacggcggcg
      361 cggcaaacga atacgagttt attttcaccc cggacggccc ggaagagtat cctgacttga
      421 cccaaacaga cccggcaaaa gcccgcgtcg gcgttgcgca gcatatcgcg tcgcttggag
      481 tgccggtgtt ggcgatctcg ctgccgggcc attacagccg caaagcatgg ccgcccattg
      541 ctgaccgccg tcctgaattc gtgatcgacg aaattccaag cgctgaggag ctcaaaaatc
      601 gtctcgccgt ctacaccttc agaatgtgcg tggaggcgat caaaaaactc gtcgaaaaaa
      661 ctctcgaaga atcgattttc atgtggggcc attcgaccgg cggtgaatat ttttacctca
      721 tggagcagtt tggcctcaaa aacaaattga tcggcggatt gggtttcggc accggaatgc
      781 cagcgtgggt gcgcaaggag tgggatttgg cctgcgcaag aaaatctccc gaagagcgtg
      841 ccgcgccgtt tcgcaacctg acggacttaa gccgccgctc accggcgggt tacgtgaaga
      901 gcggctacat cggtccgaat cagccgtggg gcagcgccga acggtggttc gagcttgaaa
      961 atcatcgtcg cccgcaattc aagccgtttt tacaagacat cgagcatagc gcccacgatg
     1021 tgctgctgcc ggacatcaag aaaatttccg gcctcgccga cgaggatttg ttcatcacct
     1081 acaaatccga cctgcatcgt ctgcgcggca aaaaactact gcacatcgtc ggagaatacg
     1141 acaagggaca ctggatcgag ggcggcgagc gaggattgca gttccggcgc gaggtgtacg
     1201 catttcaccg cttcaaaccg tacgccgacg ccctgcgtct gatcgtcgtg ccgaatctga
     1261 cccattacgg ccacgtcgaa agttataatg agcggctggc aaacttgatg gtcaccggct
     1321 tcaaggaata tttctgcggc taatctcctg gaatgcgcca gcttgaatcg tttgcttgac
     1381 agggtcggcc atctaaccta cagattgctc tcgatgaaaa gttccacgca gcgcgctcgg
     1441 gcaacttttc gccgcatgct ctcgcactcc gtggcaatag tcgttctgtt ctccgccgtt
     1501 tgcgtggcgg cggagccgtt gcgcatcgcc tacacttcga tcgcagtagt ctacggcccg
     1561 ctatggctca ccaaagaagc gggcattttc aagaagtaca atattgagcc ggagttcatc
     1621 tatatcgccg gcggtccacc gtctctgcag gcgctcattg ccggcgatgt cgcgatctct
     1681 ttcactgccg ccggcgcaac cgtagcagcc aacctgcaag gttcggacgt cgtgctcctt
     1741 ggcgcgacga tcgattcatt gccgttcgag ctttggtcca tcccgaccat aaaacagccc
     1801 gagcaactca ggggaacaaa gctcggcgtc agccgtatcg gcgcgaccac tgatttcatc
     1861 gcccgctatt tgttgaaaaa atggaatctc caacccgata aagaagtcgc gatttttcaa
     1921 accggctccg gaccgcaggt cttcgctgca ctcaagggcg gcgcggttca aagcggcgtg
     1981 ctctccaccg gaccggagac actcaacgcc gaagcggcgg gcttttttcg tctcgcggat
     2041 gtttcgagct ccggtttggt ctatccattc ggcccctttg ccgcacgcca ggcttttttg
     2101 aagacgcacg cggctctggc gatgcgattc atgaaggctt atgtggaagg gattcatcgc
     2161 ttcaagaccg acaaagcggc ggcgttatcg gtgttagaca aatataccaa gcaaaaggcg
     2221 accccggccg ccgaaaagat ctacgaactt tacgcgacta aatacttcaa gcgcgctccc
     2281 gaagcgacct cagccgcgat ccaaacgatt ctcgaagaac tctccgccac gcgtaccctg
     2341 cccgccggta ttacgccgca gcgcttcacc gaatcgagat ttatcggcga gctgatacgc
     2401 gacggttttg tcgattcgct ctacaagaac cggtgatgac ttcaggagag gcgcttatgc
     2461 cgatcaaacc gatcgtggcg cagctggaaa accgtagggg tgaatacgac cgaaaacacg
     2521 gtggacgtgt tgatctccga gtcataatgc accggcatgc cgcaaccaag gagcaatcgc
     2581 catttcgatc gtgatcgtcg ccatgccata cggcattctg gaccctgaac ggttgtcgaa
     2641 ggcatcgcgc atcagcatag aacgggccat cgactggtgg caaaaatcca tgctgacgtc
     2701 caagcacaag acctatttcg tgctggcggg ttacgatagc gatcagggac aggaaattaa
     2761 gctgcgccgg gcgatcatcg aggagacggt ccatgagccc gagctgttgg cgaacctgat
     2821 cgaagtctcg gctcgaaatg aaaccgctct agcacaaaag attgctcgcg tccgcgctct
     2881 cctgccggtt gagaccatca ccgtgtttgt cgaatcgcga aatgccgtaa gcgtcaaggc
     2941 catctttaag cgtaagttcg gtaaaacgct acaaatccgg aaatttaaag cgacatttga
     3001 attcaatcac cagtggatta cgacctcgac atccttcgcc tggttttccc gcaactggtt
     3061 tt
//
LOCUS       DASULZ010000255         3814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_437967, whole genome shotgun sequence.
ACCESSION   DASULZ010000255 DASULZ010000000
VERSION     DASULZ010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437967"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="VFY96_05325"
     CDS             complement(<1..1001)
                     /locus_tag="VFY96_05325"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX5605915.1"
                     /translation="MKTHLGIASLLVLRVFLAPVSVPAQQPTTLYVNRTDSTCAGHSP
                     CFRTIQAAIDSAVPASTIRIQAGTYPEQLSISDKNNFQSASEIDRIVVEADPGAGSGS
                     VVLTGSAGPQCTDKFAIRLKQSKFITIRGLTITGTGGQAISLLGGNNQNQDIHIELNR
                     IFANGSSSCNGGITVARGNLGTLIVNNLLYGNGRNAITFMDADGGPHYVINNTIWGNQ
                     WNGIEVARNHIVTLANNIVNNNGTASGTTGGRFGVIRESSNTPQPAGIKLLNNFVCGN
                     AQGQISAQVLDSTDSSNFTPVGNEGPGVGALPGCQLSGNLFGNVNGTDKQPNTADDD"
     gene            complement(1301..3115)
                     /locus_tag="VFY96_05330"
     CDS             complement(1301..3115)
                     /locus_tag="VFY96_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX5605916.1"
                     /translation="MHGFHGRLLHIDLTSGRSVWRDIEASRLRAFLGGIGLGTSLLYD
                     YAPAGVDPFSPANPLIFASAPLVGTGLTTTAKYAVVTKSPLTGFIADSLSSSYFALEL
                     KRIGVDALIITGRSNAMSYLSIKNQTVEIRSAGHLLGKSPLETESIIRAELNWPSARV
                     AAIGKAGENLVRFATISNEGRHAGRGGVGAVMGAKNLKAIALSGECPTKVADANSLEK
                     IAGALRQRSLSSLTDKYREIGTVANLSVFNRLGTLPTRNFQQSTFDDADAVSGESLTE
                     NNFSRRHGCAACTIRCERLFKALDGRDQRMEYETLFALGPLCGIRDPDSVLHAAQLCD
                     LYGLDTISTGGTLAWAMECGDKALLPEAQKFGLHFGEPEGIFAAIAAIAERRGIGALL
                     AEGSRRAATEVGGDSSDWAMHVKGLELPGYEPRSLKTMALGLAVSPRGACHNRSGAYE
                     ADFSGEVDRFSIDSRRGSLVAESENFAATLDSLIVCKFLRKCFNDYYQEAAEVLSKAS
                     GWDYSGDELKRAGERIHNLKKLYNIREGWSTDDDWLPERLLSEALPTGVGAGVRLNPD
                     ELRQMIRSYYQARGWDDAGLIPESKLAELNLPHLPPHT"
     gene            complement(3118..>3814)
                     /locus_tag="VFY96_05335"
     CDS             complement(3118..>3814)
                     /locus_tag="VFY96_05335"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX5605917.1"
                     /translation="LPAVLVHDEVAYQGPVSGIFHGLKVSTHEVCFVTSCDAPFLNLE
                     LIAYLLSQITEHDVVVPQWQERLQPLHAAYRKAVAPLLKAQLERGELRPVFLYDKVRT
                     RKIHEDEIRRLDPEGLSFLNMNSPAEYDAALQLWRKRNSISVSVELFGVPRMLTKTQT
                     VSLDLREGATLAQVFLALGEKLPALVGRVLKSGRLINGYSCNVNGLNFVRAPDVKVSA
                     GDKIFILSADAGG"
BASE COUNT          834 a         1036 c         1117 g          827 t
ORIGIN      
        1 aaatcgtcgt cggccgtgtt cggttgtttg tcagtgccgt tcacatttcc aaacaaattg
       61 cccgaaagct gacagccagg cagcgctccg acgcctgggc cttcatttcc gaccggtgtg
      121 aagttgctcg aatccgtcga atcgagcacc tgcgcagaga tctgcccctg cgcatttcca
      181 cacacaaaat tattgaggag cttgatgccg gcgggctgcg gtgtgttaga gctttcccgt
      241 attacgccga atcttccacc cgtagtgcct gaggctgtgc cattgttgtt gacgatgttg
      301 ttggcgagcg tgacgatatg gttgcgcgcg acctcgatac cgttccattg attgccccaa
      361 atggtgttgt tgatgacgta atgcggcccg ccgtcggcgt ccatgaaggt aatggcatta
      421 cggccgttgc cataaagaag attgttcaca atcagagtac ctagattgcc gcgggcgacc
      481 gtgatgccgc cgttgcacga gctgctgccg ttcgcaaaga tccgattgag ctcgatgtga
      541 atgtcttgat tctgattgtt gccgccgagc agagagatcg cttgtcctcc ggtcccggta
      601 atagtcagtc cgcggatggt gatgaacttc gattgcttta gccgaatggc aaacttgtct
      661 gtgcactgtg gccccgctga accggtgaga actaccgaac ccgatccagc cccggggtct
      721 gcttcaacga cgattcggtc gatttcgcta gcactctgaa aattgttttt gtccgagatc
      781 gaaagttgct cgggataggt gccggcttgg atgcggatgg tgctggcggg aacggcagaa
      841 tcaatcgcag cttggatcgt cctaaagcaa ggagaatggc ccgcgcaggt ggaatcagtc
      901 cggttaacgt aaagcgtggt cggctgttgt gcgggaacag aaacaggtgc caggaaaacc
      961 cgcagaacta acagcgaagc aatacccagg tgtgttttca aagatgcctc cgctccgcag
     1021 atatgttggg cgatgtttta cataaaaata aaagttcccg ggctaatagc aggggacgga
     1081 atataatgtc aagtgagtga atgccctgct tgcagacgaa ctacagcgac gagaaaatag
     1141 tgctcgttca gggttgtatc agattagccg agagatgatg cgctatttga actgatcgag
     1201 gcgagacgcg gtcggaacat ttgtgctgga aacgttccag aatcagaggc gaaaacagtt
     1261 ctctagatta ttatttgctg acccggattg ctggaggagt ctaggtgtgc ggcggcaggt
     1321 gcggcagatt gagctcggct agtttgctct ccggaatcag tccagcgtcg tcccagccgc
     1381 gcgcttgata atagctacga atcatttgcc gcagctcgtc gggattcaat ctaacgccgg
     1441 caccgacgcc ggtggggagc gcttcactca gcaaacgttc cggcagccaa tcgtcgtccg
     1501 tgctccagcc ctcacgaata ttgtatagtt ttttaagatt gtgaatgcgt tcgccggcgc
     1561 gcttcagctc gtctcccgaa taatcccagc cggacgcctt gctcagcacc tcggcagctt
     1621 cttggtagta atcattgaag catttgcgca ggaatttaca gacgatcaac gagtcgagag
     1681 tggcggcgaa attttcagat tccgcgacca agcttccacg tcgagaatca atgctgaagc
     1741 gatcgacttc gccggagaaa tcggcttcgt aagcgccgga acgattgtgg caagcccctc
     1801 tcgggctgac cgccaatccc agcgccatgg tttttaggct gcgcggctcg tagccgggaa
     1861 gctccaggcc tttaacgtgc atagcccagt cgctgctgtc gccgccgact tccgtggcgg
     1921 cacgacgcga gccttccgcc aagagcgcgc cgattccccg gcgctcggcg atggcagcga
     1981 tggcagcgaa aattccttcc ggttcgccga agtgaagccc gaacttttgc gcctcaggca
     2041 gcaatgcttt gtccccgcat tccatcgccc aagccaaggt gccgccggtg ctgatggtgt
     2101 ctagcccgta aagatcacaa agctgcgccg cgtgaagaac gctgtcggga tcgcgaattc
     2161 cgcagagcgg gccgagggca aaaagggttt catattccat ccgctgatcc cgaccatcga
     2221 gcgctttgaa gagccgctcg cagcggatcg tgcaagcggc gcagccatgc ctgcggctaa
     2281 aattattttc cgtcagcgac tctccgctca cggcatcagc gtcgtcgaag gttgattgct
     2341 gaaagttgcg tgtcggcaga gtgcctaagc ggttgaatac ggacaaattg gcgacggtgc
     2401 cgatctctcg atatttgtcg gtgagcgagc tcaagctacg ctggcgcagc gcgccggcga
     2461 tcttttcgag gctattcgca tcggcgactt tggtcggaca ctcaccgctt agagcaatgg
     2521 ccttgagatt tttcgctccc atcactgcgc cgacgccgcc gcggccggca tggcgacctt
     2581 cattactgat ggtcgcaaag cgcacgaggt tttcgcccgc tttgccgatg gcggcgaccc
     2641 gcgcgctcgg ccagttgagc tcggcgcgaa tgatggactc ggtttcaagg ggactcttgc
     2701 ccagcaggtg tccggctgag cgaatctcga cggtttggtt tttgatcgag agatagctca
     2761 tggcgttcga gcggccggtg atgatcaatg catcaacgcc gatgcgcttg agctcgaggg
     2821 caaaataact ggaggagagc gagtcggcga tgaatccggt gagcggtgat ttggtgacga
     2881 ctgcatattt tgccgtcgta gtaaggccag tgccgaccaa aggagcactg gcgaaaatca
     2941 atgggttagc gggtgagaag ggatcgacac cagccggcgc atagtcgtag aggagactcg
     3001 tgcccaagcc gatgccgccg agaaacgcgc gcaagcggga tgcttcgata tcacgccaaa
     3061 cgcttcgtcc ggaggtcaag tcgatgtgga gcaagcggcc gtggaaaccg tgcatggtca
     3121 gcctccggca tccgcggaaa gaataaaaat cttatctccc gcactgacct tgacgtccgg
     3181 cgcgcgcaca aagttcaagc cgttgacgtt gcaactgtaa ccgttaatca acctccccga
     3241 cttgaggact cggccaacca gagccggcag cttttctccc aaggctaaaa aaacctgcgc
     3301 cagcgttgct ccctcgcgca agtcaagcga gaccgtctgg gttttcgtca acatgcgcgg
     3361 cacgccgaaa agctccactg aaacggatat cgaattgcgc ttgcgccaga gttgcagcgc
     3421 ggcatcatac tccgccggcg agttcatgtt gagaaagctc aaaccttcgg gatcgagccg
     3481 gcggatttca tcttcgtgaa ttttgcgggt ccggactttg tcgtacagaa aaaccggtcg
     3541 cagctcgccc cgttcgagct gcgctttcag cagcggggca acagctttgc ggtaagccgc
     3601 atggagcggc tgaaggcgct cttgccattg aggaaccacc acatcatgct cggtgatctg
     3661 cgacaaaaga taggcgatca attcgagatt caaaaacggc gcatcgcagg aggttacgaa
     3721 acaaacttcg tgggtcgaaa ctttgagtcc atggaagatg ccgctcactg gaccctgata
     3781 ggcgacctcg tcatggacca gcacggccgg caat
//
LOCUS       DASULZ010000256         5183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_843688, whole genome shotgun sequence.
ACCESSION   DASULZ010000256 DASULZ010000000
VERSION     DASULZ010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5183
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843688"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VFY96_05340"
     CDS             <1..368
                     /locus_tag="VFY96_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605918.1"
                     /translation="NIHGFRVDMTGQGWFDHPSDDVDLCATPMEPFRQQAERADKRIF
                     FTTLSTEHIPNEQILPQLLALEPVTTIGYPIGLWDEINNLPIARRGMTATHPRVDFNG
                     ERIGLIDIAAFPGSSDRQC"
     gene            901..1248
                     /locus_tag="VFY96_05345"
     CDS             901..1248
                     /locus_tag="VFY96_05345"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX5605919.1"
                     /translation="MVQFKILSVFVAVAFVGAFSTSNYAAERGKKPVAADTVRIPAPT
                     IIKPVDINSGSIEQLRALPGIHHSYARKIIDGRPYQKKQELVSRKIIPEPVFEKIKDR
                     IITVNQEARPPSD"
     gene            2536..2970
                     /locus_tag="VFY96_05350"
     CDS             2536..2970
                     /locus_tag="VFY96_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5605920.1"
                     /translation="MGQRERQTILIIEKQPNYTQLLVKQVIFAGFRPLVAVTGEGGIR
                     NAIEHHPDLILLELDLTDMDGLAFVSLLREEPQVQKTPIVAMSIFSYLKSPALFGGCD
                     DFLQKPVKMIELMGHIRRLLQEPALACKTPNLVSRVPRSALS"
     gene            3311..4129
                     /locus_tag="VFY96_05355"
     CDS             3311..4129
                     /locus_tag="VFY96_05355"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HEX5605921.1"
                     /translation="MPVVTIAHAAYSGGSAIAQRVASLLNYRSIDREVLIEASKRYGI
                     PEAKYTEVLETEGHWWERWRESVRLYRITLQAAMCEVVEGGDLVYYGRAGQQLLPGIA
                     HVLKVLIVAPMEYRIEQVGLQKGMSSENARQYIRELDRVRSRRFRAVFNDEWQNPVGH
                     DLVLNSARFDTELGASLIAETVARPEFQPTADSAKAFQDLATTAHVQAALITSPKTRN
                     VVLNVKSDSGQVTISGILADPDLEKEILRIAAAVPGVSHVITDIEPPPIEYMHP"
     gene            4176..4700
                     /gene="tadA"
                     /locus_tag="VFY96_05360"
     CDS             4176..4700
                     /gene="tadA"
                     /locus_tag="VFY96_05360"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014554163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEX5605922.1"
                     /translation="MITSASEYMRLALAAARQAASRGEIPVGAVIVSAGQVIATAYND
                     REERQTPLGHAEITAIQLAAQYLGSWRLDNAELYVTLEPCIMCAGAILQARISRLIFG
                     CRDPKAGAIESLYRLCEDQRLNHQLPVTAGVLANESAALLRDFFARLRIQKSDTRNCD
                     PAAQNPPRTGHPAK"
BASE COUNT         1232 a         1321 c         1372 g         1258 t
ORIGIN      
        1 agaatattca cggatttcga gtcgacatga ctggtcaggg ttggtttgat catccgtcgg
       61 atgatgtaga cctctgtgcg acgccaatgg aaccatttcg ccaacaggcc gaacgagccg
      121 ataaaagaat cttttttaca accttgtcaa ccgaacatat tcctaacgaa cagatcctcc
      181 ctcaactgct cgcgctagag ccagtaacga cgatcggcta tccaatcgga ctttgggacg
      241 agatcaacaa tttacctatt gctcgacgtg gtatgaccgc gacgcatcca cgcgtagact
      301 tcaacggtga aaggatcgga ctcatcgata tcgctgcttt cccgggttca tcggatcgcc
      361 agtgctgatt gttgacgagg gcggttatgc tatcccaggt gggttcgtga ctggcggccg
      421 gcgcattctc ctgctcggaa tcctgtacgc cgggccgcag ttcacagcgg acggctcact
      481 tgagatagag aacattccta cgacgtggga cagcgcgttt cagcccagaa ctctctgttc
      541 atctcgggtt ctatataagt cagaagagtt gtttgaatta ggcgaggtga tttccgctgc
      601 cgatgccaaa acaccttagc ttgttcattc aagagaatgt gtccatcgcg ccagcttttc
      661 tgaacttctg ccaaccgaac gaaaggccgt aagatattga ttcgattaga tgcggtgagc
      721 ctggtacaat ctgacaaaag ttgctgctcg attctatgga tcggggggtt ggggttcaaa
      781 tccctccggg cgcgccatgt ttagccatca aaacatctca catcacgtaa tattgtagcc
      841 aaacgaacgt ctaatctagc gggcaacttc acgggtacgg gtactcgaga atagagctag
      901 atggtgcagt tcaaaatctt gagtgttttt gtagctgtag ctttcgttgg ggcattctcg
      961 acttcgaatt atgcggcgga acgcggtaaa aagccggtag cggctgatac cgtccgaatt
     1021 ccggcgccca ccataatcaa acccgtggac atcaactccg gatccatcga gcaactcaga
     1081 gcgctgcccg gaattcatca ctcatatgcg cgaaaaataa tcgatggtcg cccttatcag
     1141 aaaaaacaag agctggtctc gagaaaaatc attcccgagc cggtttttga aaaaattaaa
     1201 gatcgtatca ttacggtgaa ccaagaagca cgaccgccca gtgattagtg cgacgattca
     1261 tatcgcgacg cattcgcgcg tagcgcgtaa acgccgctgc ggaaaaggtt cgcgctccac
     1321 cgagacgctt ccgcccgccc gcaaaaaatt tgtctgtgcc agtcatgtga cgcgctaact
     1381 tgttagtgcg ttcggctaca acattaggca gtttgccacc ttcctttgtg cttcaattac
     1441 gcatgccgcg tcatgatgtc atcagatttc tgtagtcgtc attttgccca ctgacggtac
     1501 ggcagttgct ttaattcgta tcgccggggc gggaaatcac agctggatac cgcatcgcgt
     1561 aatctctccc ccggcacctc tcaacacgtc tttatcgcgt gggcgctttc ggcaggagct
     1621 gattaactgc aggaagcatt tttgtacgtc tcttgctctt ggtctcaatg gagcgaacgc
     1681 ggcagaattc attggttatt tcaaatggga agtagaaaaa agctagttga gcccgggaag
     1741 aatcgcgtca gacgatctgg aggacggcag ctgatcggcg aggaaatagg gccggcacgc
     1801 gttcgccaaa cgagattgtt caaaacggag ctggacgcgg aaatgtgggt gagagcggct
     1861 cctggacgac gctcgcgact ggttatgatc ggaagcgcgg catggatgcg cgcactggat
     1921 gcggaattga aagtcgcatc cgctgaaaaa taaactagca cgctggcaag tgtcagagcc
     1981 taaagattag tcgctgagct attgcgaagc ttacggcagc gccaccgtgc tcttgaccat
     2041 cctcgaggca tacgatcaca acatataaac gtaacaccaa ttttcatcgt cgcatgtatc
     2101 tcggagtctg gctgtgatcg agaccggtca agcgccggcg cgtttcggaa gcaaaaggca
     2161 agcgccgagc gtttttttaa ccggctcgtc gaccagttta ccgagcgaga gtgcaatgga
     2221 atcagattta gttgccctat ggcttaggtg tagaagggaa gcctgagagt ccacggatgc
     2281 tgacgatacc gttagaaaag aatctacggt taagtgagaa atttgaactg tcgatttttg
     2341 aaagcgtcgt aaaacgcaca gccgtcaggc cagatcgatt tccttgagac gctcggcggt
     2401 aatgcctttc aggtaagttt tatctttaat ggggtaattt tttgtgcgcc agcacgttct
     2461 ttcctcttcg ttaaaacggt agggccgtta ggggcatgtt tgttgctgct tgggcgctgg
     2521 cggggtcaaa gctcaatggg tcaacgcgaa cgccagacaa tactgattat cgaaaagcag
     2581 ccgaattaca cccaattgct tgttaagcag gtaattttcg ccggctttcg gccgctcgtc
     2641 gcggtgaccg gagaaggtgg cattcggaac gcaattgaac accaccccga tctcattttg
     2701 ctcgaattgg atttgaccga catggacgga ctggcattcg tttccttgtt gagagaagaa
     2761 ccgcaagttc aaaaaacacc aatcgtcgca atgagcattt tttcctacct gaaaagcccg
     2821 gcgcttttcg gcggctgcga tgactttttg caaaaaccag taaaaatgat cgagctcatg
     2881 ggtcatatta gacggctctt acaggaacct gcgctcgcct gcaaaacgcc caatctcgtg
     2941 agtcgggttc cccgctcagc gctttcctga tttttttcag ccaaattatt tcagcttacg
     3001 ccgccccgca gaaaagcagt agtgctctct gctttaccta tgaggtgccg gtgatttcgt
     3061 atttttcgaa ttgtcatagc gatgcttcca ccgtttcgcc atacctagaa tccggagtgg
     3121 accccgatta aaccctaagg ggagttttgg cgttctactc ccagaaatat tttcagagtc
     3181 tcttagggca gcaatgccaa acgccgcaca tcaagagctt gtccgaaccg cgggaaaaag
     3241 gtagtaaata gccggttaat tactgtcaag gctgcggcgc tagctggacg ctaacaggag
     3301 ggctgaactc atgcccgtcg tcaccattgc tcatgcggca tatagcggcg ggagtgccat
     3361 tgcccaaaga gtggcatcgc tgttgaatta ccgttccatc gatcgtgaag ttttgatcga
     3421 agcgagtaag cgttatggga ttcccgaagc caaatataca gaagtgctcg aaaccgaagg
     3481 gcactggtgg gaacgttggc gcgagagtgt tcggctttat cggatcacct tgcaggcggc
     3541 aatgtgcgaa gtcgtggaag gaggcgattt ggtttactat ggtcgcgccg gacagcagct
     3601 tttgccggga atcgcgcacg tcttaaaagt attgatcgtc gcgccgatgg agtaccggat
     3661 cgagcaggta ggcttgcaga agggaatgag tagtgaaaat gcccgccaat atattcgtga
     3721 gttggatcgg gtccgcagcc ggcgttttcg tgcggtgttc aatgatgaat ggcaaaatcc
     3781 ggtgggtcac gacctcgtct taaacagtgc gcggttcgat actgagcttg gagcatcctt
     3841 gattgccgag accgtagctc ggccggaatt ccaacctacc gccgactcgg cgaaagcttt
     3901 tcaggacttg gcgaccaccg cgcatgttca ggcggcgctc atcacatcac ccaagactcg
     3961 caatgtggtt ctcaacgtga agagcgacag cggacaggta acgatctctg gtatcctcgc
     4021 cgacccggat ttggaaaagg agattctccg aatcgcagca gcggttcctg gcgtctctca
     4081 cgtgattacc gatatcgaac cccctccgat tgaatacatg cacccgtagt cgctctccga
     4141 gcgatctacc caatttgttt taagtagctt tgatcgtgat cactagcgct tcggaatata
     4201 tgcggctcgc gttagccgcc gctcgacaag cggcaagccg tggcgagatt cccgtgggcg
     4261 ctgtaattgt atccgcggga caagtcattg cgacggccta caacgaccgt gaagagcgtc
     4321 agacgcccct aggtcatgcg gagatcacag ctatacaatt agcggctcag tatctcggga
     4381 gttggcgtct cgataacgcc gagctttacg tcactctaga gccatgtatc atgtgcgccg
     4441 gcgctatttt gcaagcgcgc ataagtcgcc tcatatttgg ctgccgagat ccgaaggcgg
     4501 gcgcaatcga atcactttac cgactctgtg aggatcaacg tctgaatcat cagttgccgg
     4561 ttaccgcggg cgtgctagcc aatgaatctg cggcgctctt gcgcgatttt tttgcccgct
     4621 tgagaattca aaaaagcgat actcgaaatt gcgatccggc tgcgcaaaac ccaccgcgaa
     4681 ccggtcatcc tgctaagtaa tcccctctac gggagagctt gccggagcgg tacaagcgct
     4741 cgacgagtta ccccggcgct caggatcggt tgcagagctg taaagctctt gccgtggcga
     4801 ggaatctcgc tattccgtag aattttcggc tcagacactt cccgctgcca tagtgcgatg
     4861 agattcctgc ccgacgtcaa ttcgcgcttc taaaccttct ccctttactt tgttggaatc
     4921 tgatcgagct cgcggcagga gagttactta tcgcctcgga aaaactattt cgaaaaatgt
     4981 gtaggcgtgt ttttgatacc gccattccgc tgctaacagt gccattctcg gttctggaag
     5041 ttcatcagtc gtaatagccg attaggcaag ctcaagccca cctatttgca accgtcgtaa
     5101 ctactttaaa tgaataaagc cgctcgcgtt ttctcgatgc ggacttttcg ctctcccgag
     5161 agtcggtcgg caactggtta ggt
//
LOCUS       DASULZ010000257         4167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_234387, whole genome shotgun sequence.
ACCESSION   DASULZ010000257 DASULZ010000000
VERSION     DASULZ010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234387"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VFY96_05365"
     CDS             <1..230
                     /locus_tag="VFY96_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605923.1"
                     /translation="MKRYDAVLTTINMGPIATAALPLPLDRRSRIGHCTWMQNVGAAW
                     LMTSLAPSPTMGGAGGYHPAWISPVSLCRCF"
     gene            complement(438..1364)
                     /locus_tag="VFY96_05370"
     CDS             complement(438..1364)
                     /locus_tag="VFY96_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX5605924.1"
                     /translation="MELVFFDDFKLGVFNGEAVVDVSHLVREVPHTGPHDLINGLIER
                     FNDYRSTLEKAASGKGVPLGTVRIRPPLPRPINIDCMAVNYMEDSTRKEPAPINAFHK
                     SPTCIIGDGDTMILPDVPAAIFEGEAELAVVIGKRADNVPAAKAMEYVFGYVNFIDGS
                     ARGLPPAGNTFYQMKSRATFGPMGPYLVTADEIKDPHGLQVKLWNNGTLMQNYHTSDM
                     THKIPRCIEWVSSIHPLEPGDVLACGTNHRGLNPFQDGDLIELETEPLGRLHIKIRDD
                     LKRTWARETRLQRQQKSLEGFTPQLTGKYAKT"
     gene            complement(1389..2036)
                     /locus_tag="VFY96_05375"
     CDS             complement(1389..2036)
                     /locus_tag="VFY96_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX5605925.1"
                     /translation="MDPKDFLNELLLPVHEFCRTLDAHPPAYSYIPLHTDEQRIRVMQ
                     SRLFNELRAADLFGTWLKTTPELDVKATMAESAHEEMVHAQLLSKRIYDLGGEPFDYK
                     PLPAQIAMFNAMEGLNDTCERVAGLSLAGETVANYLIQMCLKSPDVPEWIKQPYRKIT
                     EDEEGHGSAPQEFLKRYATSDDRQDRARRAVAMRMVLFREYLASLDRWAMGIDRW"
     gene            complement(2115..>4167)
                     /locus_tag="VFY96_05380"
     CDS             complement(2115..>4167)
                     /locus_tag="VFY96_05380"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5605926.1"
                     /translation="LCFMVLTVFVWFQSTFDFVTESILPKELRIFALSLTPSAGIHLA
                     LLLSSGSSGASLRPLHIALVYGISLLLGTVNSVTFFGPLDTWIHNFRAGYLYICIGAI
                     SFLVITGNALRRTESELDRLRLRVMFVGALVGFFVPGAATVLTSTYHFRIPYNLALVP
                     TIFFPVSVAYALLKYSLFDLGNALRLTLSRFALLTLLVAIYAVLALLIAPWIGDYAKD
                     PLVPLFFSILVVILFNPLLRWLEGIIDRYIFRQDYDPATVQEEISLYLRNLDSAASLA
                     KGFIDRTTRFLEIESAIVTYRHKQSAAHLAVAMGSALSIPEQLIANSEGVRELWPAHD
                     YRAVARAEISNHPSYQESRNAVLGVFDRWQAELLMPLVYEREVRGVVAFGARRSGREY
                     SAEDYRLLGTLVQQLALSLENGRLYEESLQAYQRVEATNRKLIEADRIKKDFVANICH
                     ELRTPVSTIIGFSEVLRDPNFKGDSRDILNRLVNNGQELSELMDNLMNFSRMETDAAA
                     AQFELVKLREILAALEMMTQRLIRERPIEFGIHIEAPVETIESDGQKLQQILVQLLTN
                     ALKFTEKGRIELSIRTRMHDSEDYLEIAVADTGIGIKHEDQKIIFDDFRQLDGSSTRQ
                     YGGTGLGLGLCRKLAAALGGEIRVASELGVGSVFSLLLPIRSPAPRAQIVHEAELQLL
                     H"
BASE COUNT          953 a         1153 c         1133 g          928 t
ORIGIN      
        1 acatgaaaag atatgacgcc gtcctcacaa ccatcaacat gggcccgatt gcgacagccg
       61 ctcttccgct ccctctggat cgccgcagta ggatcggaca ctgcacttgg atgcaaaatg
      121 tcggtgctgc ctggctgatg acctccttgg ctcccagtcc caccatgggt ggtgcaggcg
      181 gctaccaccc tgcgtggatc agcccggtct cactttgccg gtgtttctga tcgggctccc
      241 gctggcaaga agaaaaataa acacaagagc gaacttgctt cgaagaaatt tcatgagatg
      301 aaaagtttcc tcccgatcgg cgtttttccc cggtcgaagg accctcgtgt tcgtaatcgg
      361 tagcggcaga ttcttcgctg acgccagagt gacccctgaa agaaaatttg cgctgaatag
      421 cagtttcgaa tctgcttcta tgtttttgcg tacttgcccg tgagctgcgg tgtaaatcct
      481 tccaagctct tttgctgcct ctgcagacgc gtctcgcgcg cccaagtccg ttttagatcg
      541 tcacgaattt ttatgtggag tcgacccagc ggttcggttt cgagctcgat cagatcgccg
      601 tcttggaacg gattcaagcc gcggtggttg gttccgcagg cgagcacatc accgggctcg
      661 agcggatgaa tggagctgac ccactcgata cagcgaggaa ttttgtgcgt catgtcgctg
      721 gtatggtaat tttgcatcaa cgtgccgttg ttccaaagtt tcacttgcag cccatgcggg
      781 tcttttattt cgtcggcggt aaccagatag ggacccatcg gcccgaaggt ggcgcgcgat
      841 ttcatttggt aaaaagtatt gccggcgggc ggcaagccac gggccgagcc atcgataaaa
      901 ttgacatagc cgaaaacata ctccatcgcc ttggccgcgg gaacgttatc cgcgcgtttg
      961 ccgatcacga ccgcgagctc ggcttcgcct tcgaatatgg cggccggcac atcgggtaga
     1021 atcatcgtgt cgccgtcgcc gatgatgcag gtcggcgatt tgtggaaggc attgatggga
     1081 gcgggttcct tgcgcgtact atcttccatg tagttgaccg ccatgcaatc gatattgatc
     1141 ggacgcggca atggtggtcg aatccttacg gttcccaggg gcacgccctt gccgctggcc
     1201 gctttttcca atgtactgcg ataatcattg aatcgctcaa tcaagccgtt gatcaggtcg
     1261 tgcggacccg tgtgcggtac ctcgcgcacc aggtgcgata cgtcgaccac ggcctcgccg
     1321 ttgaatacac cgagtttgaa atcatcgaaa aatactagtt ccattctgac tccttactgt
     1381 cgccgagttc accaccggtc gatgcccatc gcccagcgat ccaagctcgc gagatactct
     1441 cggaacaaca ccatgcgcat cgctacggcg cgacgggcgc ggtcctggcg gtcgtcggac
     1501 gtggcgtagc gcttgagaaa ctcctgcggc gcactgccgt gaccttcttc atcctcggtg
     1561 attttccggt acggctgctt gatccattcc ggaacatccg gcgacttcag gcacatctga
     1621 atcaaataat tggcgacggt ctcgccggcg agggataagc cggcgacgcg ctcgcacgtg
     1681 tcgttcaaac cctccatggc attgaacatg gcgatttgcg ccggcagcgg cttgtaatcg
     1741 aatggctctc cgccgagatc gtagatgcgc ttcgacaaga gttgtgcatg aaccatttct
     1801 tcgtgcgctg actctgccat agtcgctttg acgtctagct ccggcgtcgt ttttagccag
     1861 gtgccgaaga ggtcggcggc tctgagctca ttaaaaagcc ggctctgcat cacccgaata
     1921 cgctgctcgt ctgtgtgcag cggaatatag ctataagccg ggggatgagc atccaacgtg
     1981 cggcaaaact catgcaccgg gagcagaagc tcatttaaga aatctttcgg gtccatgatt
     2041 aatctccctt ttggaaagct ctcgaaccgt cacaccttat cactctcact atgcgaacac
     2101 accaatgtgc gcaatcagtg cagtagttga agttccgctt catggacgat ctgtgctctc
     2161 ggcgccggcg accgaattgg caagagcagg ctgaagacgc tgccgacccc aagctcgctc
     2221 gccacacgaa tttctccgcc cagtgcagcc gccaattttc tacataaccc cagtcccaat
     2281 cccgtgccgc cgtactggcg agtcgatgag ccgtcgagct gacggaaatc gtcgaagata
     2341 attttctgat cttcgtgttt aatcccaatg ccggtatccg caaccgcgat ctccaaatag
     2401 tcttcgctgt cgtgcattcg tgtgcgaatc gatagttcaa ttctgccttt ttcggtaaat
     2461 ttgagggcat tggtcaggag ctgcaccaaa atctgctgca atttttgccc atcgctttca
     2521 atcgtttcga ccggggcctc gatgtggatg ccgaattcaa tgggccgctc gcggataagt
     2581 ctttgggtca tcatttctag cgcggcaaga atttcgcgca gtttgaccag ctcgaattgg
     2641 gcggcagccg catctgtttc catccgggaa aagttcatga ggttatccat gagctccgac
     2701 agctcttggc cattattgac cagacgattc aaaatatcgc gactgtcgcc tttgaaattg
     2761 gggtcccgca aaacctcgct aaaaccaatg atcgtgctta ccggagtgcg taattcgtga
     2821 caaatgttcg ccacgaaatc ttttttgatc cgatccgctt cgatcaattt gcgattggtt
     2881 gcttcgacgc gctggtaggc ctggagagac tcctcgtaga ggcgaccgtt ttcgagagag
     2941 agcgccaatt gttgaaccag agtccctaac aatcggtaat cctctgcgct gtattcccgt
     3001 cccgaacgac gggcgccgaa agcgacgacg ccgcgcacct cgcgctcgta caccagcggc
     3061 atgagcagct ccgcctgcca acgatcgaat acgcccagaa ccgcattgcg gctttcctga
     3121 tatgaggggt gattgctgat ctctgcccga gccacggcgc ggtaatcatg cgcgggccaa
     3181 agctcgcgaa cgccctcgct gttggcgatc agctgctcgg gaatactcag agcggacccc
     3241 atagctacgg ccagatgtgc ggccgattgc ttgtgccgat aggttacgat ggcactctcg
     3301 atttcgagaa agcgcgtagt tcggtcgatg aagcctttag ccaaagaggc ggcagagtct
     3361 agatttcgca gatacaaact gatttcttct tgcaccgtgg cggggtcata atcctggcgg
     3421 aaaatatagc gatcgatgat accctccaac cagcgcagca atgggttgaa tagaatgacg
     3481 acgaggatgg aaaagaacaa aggtacaagc gggtctttgg cgtaatcgcc gatccagggt
     3541 gcgatgagca gagcgaggac ggcgtagatc gccaccaaca aggtcaaaag ggcaaacctg
     3601 cttagcgtca accgcaaagc attaccgaga tcaaaaaggc tatacttcag gagcgcgtaa
     3661 gcgaccgaaa ctggaaagaa gatcgttggc acgagcgcaa gattgtaggg aatccgaaaa
     3721 tgataggtgc tcgtcaaaac cgttgccgcg cctggtacga aaaacccgac gagcgcgccg
     3781 acgaacatca cgcgcaaacg cagacggtcg agctccgact cggttcggcg cagagcgtta
     3841 ccggtgatga cgagaaagct gatcgcaccg atgcaaatgt acagataccc agcgcgaaaa
     3901 ttgtgaatcc atgtatccag cggaccgaaa aacgtaacgc tgttgactgt gccaagtagc
     3961 aagctgatgc cgtagaccag cgcgatatga agtggccgca aggatgcccc ggacgatcca
     4021 ctgctcagca acaacgccag atggatcccc gcgctcggcg tgagagagag tgcgaagatg
     4081 cgcaattcct tgggcaggat ggactcggtg acaaagtcaa aagtgctctg gaaccaaacg
     4141 aacacggtca aaaccatgaa acacaat
//
LOCUS       DASULZ010000258         3125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_640621, whole genome shotgun sequence.
ACCESSION   DASULZ010000258 DASULZ010000000
VERSION     DASULZ010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640621"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VFY96_05385"
     CDS             <1..891
                     /locus_tag="VFY96_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605927.1"
                     /translation="GYSSGETASTAMLVCFAVKIALVVLGCQKPSVTDGTKPSEIGSK
                     VEALMADKARSAGNLLWRSLFIGALFLIFQIVLAGPARGAESDPTDTLPMFGQPKINR
                     SEDLKKSDEAFIRDSTLRFGSRQAGSNTLAAQGWVAARAGQDDVAMRRFNQAWLLNPK
                     NYSVFWGFGAVLSGKGKLSEALDYLEKARELVNDPKQRVALLCDVGAVHSAYAARLPA
                     DRQLERAQQFVLANNRFSESLENDPTFAQSWREWAISLYEQERYSEAWIRVKKARELN
                     AEPFPPEFLEKLSAKMPEPK"
     gene            1002..1730
                     /locus_tag="VFY96_05390"
     CDS             1002..1730
                     /locus_tag="VFY96_05390"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEX5605928.1"
                     /translation="MLPIILAAPHGGRAAVPGLVVRRGVGVPQFTTERDSNTAELAEL
                     IGVKVAALLGTRPFLVIAHFERKFIDANRTENSAFESAAAKPYYEAYHTAMKDALKRV
                     RQRWGAGLLLDIHGQGTEPNTIFRGTNNGKSIARLRRRFGLAAIAGAKSILGQMAAKG
                     YRVEPTDDHERRYVGGYTTQSYGSHRGTEIDAIQLEFGANLRKSSNLERTATDLAHAI
                     EIFTREYLPITPMPGEDRALSVEP"
     gene            2047..>3125
                     /locus_tag="VFY96_05395"
     CDS             2047..>3125
                     /locus_tag="VFY96_05395"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5605929.1"
                     /translation="MSEKQSVDSGKAQSFAERLLTILNDGALCLMISVGHRTGLLDAM
                     NGAPAASSFEIAARAGLNERYVREWLGAMVTGRIVNIDPVTNRFSLSPEHGAFLTRAA
                     GADNIGVFAQYIALLGSVEDEIVECFKHGGGVPYARFPRFQEVMAEDSGQSALSSLES
                     HIVPLVPGLREKLTDGIQMLDLGCGRGKIINRLAELYPKSRFTGMDLSSEAVLSAWEE
                     AAQKKLRNIEFIVTDLTHFDESAEVESFDLIATFDAIHDQAKPLNVLRGIHRALRNDG
                     VYLMQDIKGSSDIHKNIDHPLAPALYTISCMHCMTVSLAQNGEGLGAMWGEEKTREYL
                     AKAGFRSVEKNELAHDIQNNWYVVR"
BASE COUNT          709 a          789 c          911 g          716 t
ORIGIN      
        1 ggctactcaa gcggagagac ggcaagcact gcgatgctag tttgttttgc cgtcaaaata
       61 gccctagtag ttttagggtg tcaaaaaccc agtgtcaccg acggaacaaa accaagtgag
      121 attggctcga aggtggaagc attgatggcc gataaagcga gatccgctgg caatctttta
      181 tggcggagtc ttttcattgg tgcgcttttt ttaattttcc aaattgtcct cgccggcccg
      241 gctcgcggcg ccgagtctga tccaacggac acactaccga tgtttggcca gccgaaaatc
      301 aatcgttcgg aagatctaaa aaagtcggac gaagctttca ttcgtgattc cacgctccgt
      361 tttggaagcc gccaggcagg cagcaatacg ctagcggcgc agggttgggt cgcggcacgc
      421 gccggacagg acgatgtcgc catgcggcgc ttcaatcaag cctggctgtt aaatccaaag
      481 aactacagcg tgttttgggg atttggcgcg gtgctgagcg gcaaaggcaa attgagcgag
      541 gcgctcgatt atttggaaaa agcccgcgaa ttggtgaacg atccaaaaca acgtgtagct
      601 ttgctctgcg atgttggcgc tgttcactcc gcgtatgcgg cgcgacttcc cgcggaccgg
      661 caattggaac gcgcgcagca gttcgttctc gccaataacc ggttcagcga gagtttggaa
      721 aacgacccga catttgcgca gagctggcgc gagtgggcga tctcgctgta cgagcaggag
      781 cgctactccg aagcttggat tcgcgtcaag aaagcacgtg aacttaatgc cgagccgttc
      841 ccgcccgagt ttttagaaaa attatcggcg aagatgcctg agccgaaata gacaaacgta
      901 tgttccggtt ttgtttattc gcgcttctaa tatcgctacc ggcgcacgct tgggccgaac
      961 aggatcagga gtccgctcgc tggcttaccg tctgggcagg catgctgcca atcatcttag
     1021 ccgcgccaca cggcgggcgc gcggcagttc ccggattggt ggtacggcgg ggtgttggtg
     1081 taccgcagtt tacgacggaa cgcgacagca atacggccga gctggccgag ctgatcggtg
     1141 tgaaagttgc cgccttgctc gggacccggc cgttcctcgt gattgcgcac ttcgagagaa
     1201 aattcattga cgcaaatcga accgaaaaca gtgctttcga gtctgctgcg gccaagccct
     1261 actatgaggc ttatcataca gctatgaagg atgctttgaa gcgcgtacgc caacgctggg
     1321 gcgctgggct gttgctcgat attcacgggc aaggaacaga accgaacacg atttttcgcg
     1381 gtacgaataa tggtaaaagc atcgctcggt tgcgccgccg ttttggtcta gccgccatag
     1441 cgggggcaaa gagcatttta ggccagatgg cggcgaaggg atatcgcgtc gaacccactg
     1501 atgatcatga gcggcgttat gtcggcggct acacgacgca atcctacggc agtcaccgtg
     1561 gcaccgaaat cgatgcgata cagttggagt tcggcgccaa cctgcgaaaa tcgtccaatc
     1621 tcgagcgcac cgctactgat cttgcccatg ccattgaaat cttcacgcga gaatacctgc
     1681 cgattacacc catgcctggt gaagatcggg cgttatcagt ggaaccttag cagcttggcg
     1741 agagagctag ctccaatttc gttagcttct atgaaattcg ctggtccttc ggcgagggcg
     1801 ctttaattcc cattgagtcg ctggcagcat cttttcataa gcttctaaat gccgtatgaa
     1861 ttccacttat cgcaatctaa tacgtagcta cgcgccgctc ttggtgcgac tggagtcgcc
     1921 gtttttttat tgctagaacc cctcgtgcgg cgattatttt tgctgcgcga cgtaagcgct
     1981 tgaagtcgcg gaggaggtca gcgattgaag gcgtgagcgc gttaagtttt cgatcgggga
     2041 gtgtacatgt ctgagaaaca atccgttgat agcggcaaag ctcagtcttt cgctgagcgg
     2101 ttgttgacga ttttaaatga cggagcgctt tgtttgatga tttcggtcgg ccatcggacg
     2161 gggctgctcg atgccatgaa tggcgcgcca gccgcgagca gcttcgaaat cgcagccagg
     2221 gcaggtctca atgagcgcta tgtgcgggag tggctgggcg caatggtgac cggtcggatc
     2281 gtgaatatcg atccggtgac aaaccggttt tcgctatcac cggagcatgg cgcttttctt
     2341 acgcgcgccg cgggcgctga taacatcggc gtatttgccc agtacattgc gctactcggc
     2401 agtgtcgaag acgagatcgt cgaatgtttt aaacatggtg gtggggttcc gtacgccaga
     2461 tttccgcgct ttcaggaagt catggcggag gacagcggcc aatccgcgct gtcatcgctg
     2521 gaaagccaca ttgtgccgct cgtgcccgga ttgcgcgaaa aacttactga cggcatccaa
     2581 atgctggatc tcggttgcgg ccgcggcaaa atcatcaatc gcctcgctga gctttatccg
     2641 aagagccgtt ttaccggaat ggatctttcg tccgaggcgg tcttatctgc gtgggaggag
     2701 gcggctcaga aaaaactgcg caacatagaa ttcattgtaa ccgacctcac tcatttcgac
     2761 gagagcgccg aagtggagtc attcgacttg attgccacct ttgacgcgat tcacgatcag
     2821 gccaagccgt tgaacgtgct ccgtggaatt caccgggcgc ttcgaaacga cggagtttat
     2881 ttaatgcagg acatcaaagg atcgagcgat attcataaaa atatagatca cccgctggcg
     2941 ccggcgcttt atactatctc gtgcatgcac tgtatgaccg tatctctagc ccagaacggc
     3001 gaaggtttag gcgcgatgtg gggagaagaa aagacgcgcg agtatctcgc gaaggcgggc
     3061 tttcgctcgg tggaaaagaa cgagttagcg cacgatattc agaacaattg gtacgtggtg
     3121 agaaa
//
LOCUS       DASULZ010000259         4952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1148763, whole genome shotgun sequence.
ACCESSION   DASULZ010000259 DASULZ010000000
VERSION     DASULZ010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4952
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148763"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1515..1718)
                     /locus_tag="VFY96_05400"
                     /pseudo
     CDS             complement(1515..1718)
                     /locus_tag="VFY96_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1743..1958
                     /locus_tag="VFY96_05405"
     CDS             1743..1958
                     /locus_tag="VFY96_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605930.1"
                     /translation="MAIYDISVHCNECGQLHAVLLRIDLDGGPDEKRSIADAFRGRLL
                     PPQLAAIKGRSAFCYKTRKKFPLSERR"
     gene            complement(2285..2734)
                     /locus_tag="VFY96_05410"
     CDS             complement(2285..2734)
                     /locus_tag="VFY96_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605931.1"
                     /translation="MLEPDYTGFTLVELFTARYSMDKFSRESSLEREIQKRCEHIRQQ
                     VNGNRGASEATAFKPYGLRVGVMLFCLTVGPFLISTFFDAISLIKGLDDTVTLSGVWA
                     LVTLPGLVMISMIGGRIDAERIIKRFKLSGDKPGENTNCFYEVINAL"
     gene            4456..>4952
                     /locus_tag="VFY96_05415"
     CDS             4456..>4952
                     /locus_tag="VFY96_05415"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEX5605932.1"
                     /translation="MEATAPVEDSFYIVATESSADHATRVLKDGDTFAVFDQHGDIQA
                     ENRTGQEGVYHEGTRFLSHLLFKLGNARPFLLNSTVQEDNLSFVVNLTNPDIYRSGHL
                     LLPRGSLHITRSKMLRQSRCFETFRFINYGLSPVEVRFSIQFGADFADIFEVRGMARQ
                     ARGSFR"
BASE COUNT         1250 a         1214 c         1276 g         1212 t
ORIGIN      
        1 cgaaacacaa gctagactat tttctttcgc ctcaggaaat caaaccgagc tgcaacttac
       61 tggtcagaag caagagccga aacgctcgat atattgggga tcagcgaaca tgcacgactc
      121 acaaatgtaa cgctcgttat cgcgccggta ctcttcccgc ttactcatgt cgtaataccc
      181 ggcgctaaac tcttcacctc gaaggccctc gacaatcgtt tgcaacgatc acacgtaact
      241 ttgatggttt aattattgat cgcgaagagt ctacataaac ttgcatcaac gtgaagtaga
      301 atcgtggtgg actaaataca cgaaataatc taggctccag gaattttcac aagggggcgc
      361 tcaaagctcg gtttccttgt tcttgctatc gttatcgagc agtaacacga aagcgacaat
      421 gcccaagcaa gaatgcctat aaccaagact gctgcgtaca ccagcaatat cgccacagag
      481 ccttcggcac cctaaaagat ggtgcggggc acggaggtct aaagatgcgg ttaccaaggt
      541 gcggctcttg tgccccagct ggaatacaag ctcagcaggg tttgtgccca atggccgaaa
      601 gaaatttcga gaactccggt caaccggaga gagtcaaagg caggtatacg ccgcggcaat
      661 attgctgacg gaattagtag cggacgaatg aggtcggagc ggcgatattt taaaaaaacc
      721 gatcaatttt ggttgggtag tccttgacga gacacttttt ccatccgcct tgtttcttct
      781 acatccttga agatgaccgt ggctccgcac gtcgtacaaa ccatcccgcc ggtcggggtg
      841 tcgtaatagt attcatgttg aacatgaggg tgtcggcatg gaagattgcc gcgttttttc
      901 cattttttcc gtagttgcat ggcatcgcgc atttgcatcg gagcgggtga gtgcgaacac
      961 catgccgctc gtactggaac gaccacaacg tttcttcaga tgccctgagt tctagctgaa
     1021 cgcttggaag atacaagcac tgctatgtcg gcgctgatca aaactgagcg gtttaacagc
     1081 ctaagctcga gcaattgttc gtgatggcgc gagagtcaag ctgtttaccg gtgaaaggct
     1141 ccggacgagg aatcgccaca atgcagcaga aaagggccac gctccacttc cggagaattc
     1201 ctcaggtttt ctcacttcag acctgagtca aattgccttc caggtgagca gccggcgtca
     1261 ctggcgaacc gatttgagcc acaatacggt ttttggtttg gcaacttggt ctcgtcatat
     1321 cgagcgccat gctttcggca gagtcagccg cagaatcaac cttaagggtt tatgcggtcg
     1381 cgaattggct agcggaacgc caccaattct tctcgaatta ttattccatg cagatccagt
     1441 agtccggagt taggtgctat cccaacgctg caaaccgcaa tagttgcaat ttttcgtgaa
     1501 tttaagcagt aagcggcagc tcgtacttgt ccccatcccg gctcggctag cgcgagctcc
     1561 accaccgctc gttcgctcgc ctcggcgatg cggttcttca gattcggctt gctgcgcgag
     1621 atctcggcca gggcggcttc gccaccggtc tcgtacaatt ctttgaaccg ataaaaactg
     1681 tcgcggctat aacccataat ccgacaggtc cgacaaactt gaactaggag gagtgtttca
     1741 aaatggccat ctacgacatc tctgttcatt gcaacgaatg cggccagttg cacgccgtcc
     1801 tattgagaat cgatcttgat ggtggacctg atgagaaacg tagcatagcg gatgcgtttc
     1861 gtgggcgctt gctgccgcct cagctcgcag ccataaaggg gcgcagtgca ttctgctata
     1921 aaacaagaaa aaaatttccg ctttcagaaa gacgataaaa tttttgttcg ttccgccctc
     1981 gaggttccga cgctactcag tcattcgata gcgtcttctg atggcgtcgt atctcggcga
     2041 gcggctcccg tttcgctaca ttggttggaa caagtattcg tgaggcgagc gagataccca
     2101 ttttgtttgc aggctgcgaa ctccagatct gatagagtcg cagccgttat caagcaagat
     2161 tggttataga cttgaaaatc aaacggacta ctggtgattt tgagatactg ttacccgtcg
     2221 gaatcggacc ccggctacag taccactcag caccaatcta cgaactcatg taggtcgcag
     2281 taaattacag agcgttgatc acttcataaa aacagttcgt attctcgccg ggtttgtccc
     2341 cggagagttt gaagcgtttt atgatacgtt cggcgtctat tctgccgccg atcatcgaga
     2401 tcattaccag cccgggcagc gtgacaagag cccatactcc tgacaaggtg accgtgtcat
     2461 caaggccttt gatgaggctt atcgcatcga aaaatgtaga aattagaaaa ggtcctacgg
     2521 ttaagcagaa gagcatcacc ccaacccgta atccatacgg tttgaacgcc gtcgcctcgc
     2581 tagcgcctct gttgccgttt acctgttgtc gaatgtgttc gcagcgtttt tggatttcac
     2641 gttcgagcga gctttctctc gaaaatttgt ccatcgagta gcgtgctgta aacaactcta
     2701 caagagtaaa gccggtataa tctggttcga gcatggctag agtcgtaaca gacgaacgtt
     2761 catgagttct cgagagcatg tccatacgct gtattcacgg agcaagcggt gtaccatgga
     2821 tcgtcagggc agggctcgcc gtaaggggtt gagatctaag aaaatgctgt cgatcagaga
     2881 tcacatttcc gatcgagtgg gccaagaagg tgcgatgcgg accattactc tccgagcaga
     2941 agccgaatcg aaaaacgttt ggtgggttct ttaggaaaca tctacatacg agaatacggc
     3001 acccgtcgac gtagcctact tcatcgaaga cggcgcaccc tcgggatgcg gcctggtctc
     3061 gagatcgacg cgtagtcata aacttactag agggatttgc aattacgcag tactaaaccg
     3121 taaaagttag tatagaacgg catcgaggtt aagccagcat cgatgctagt aagacgtcta
     3181 aggttgcttg aagcttgttt gcgaattccg ttgctatttc aaaagattct tcttcagtaa
     3241 ggaagctcga tcggtgagtt tatagaagct cagagtcgat tgattgaacc accgaggtat
     3301 gtaaacaccg cggggttgac acgccgaacc aagaagcgag tgtcgcgcca atcagatagc
     3361 cggggaaata atagaaaaca tcctccccaa atgagagaaa ggaaagacgt gccgtcagct
     3421 ttcgttagga ggcaagctcg tagccggaaa cggggcgaaa cgatgacagt cgcatcagca
     3481 agcatcatca gacgagttag ccgctcacac cgtattgcag acaaaaaagc tctgctgatc
     3541 ggcttgttaa tcaccctgtc cgggtgttca tcggtcatga gtaacagcgc acaattttat
     3601 caggatcatc cgcgcggacg aatcaattgg cgtgagtata tgacggattg attgcattaa
     3661 tcagcgtcag atctgatgca ggcatcaccg cttcggtaat ggaaaagcga gagtcgatgg
     3721 actcttctca cggaaaccac tacgcataat gaattttcgc gaaccacgca gtacgtacgc
     3781 ggtgaactag aaaaaagcgc ctttatactc gctcgagtgt ccgaaactct gcggatcttc
     3841 cgtccaactc agttgaattg tttgatcgat ttgcagagct ttgctcacat gactgctggt
     3901 cgataggtcg ttgcggcaac gcgacagatc agcgcaggtt gtctcgtcgt gtgctcgcga
     3961 ccgcagcgct ttgcagctgg aacttagtga aatggcttca tagatgtcgt cacgcgggca
     4021 aatctacgcg aaatgttagc cggctgtcgc ggcgaagatg gcgtgctcaa tgtcctttac
     4081 cgctttggga taatgatcac gggctatctg caggagtgtt tccgcctggg gcggttcaga
     4141 ttcaggaata gatttcgatg cccgctaagt ccttgcgggc ggcatgctcg agaatttggt
     4201 gcaggtccgt tccctctctg aaatgtcgga ccagcaagtc acttcagtcc gcgctcaatt
     4261 ccatggaaac ttcatagaaa cagcctagcg ccagtatggt gaaatacaat atgggcagag
     4321 ttgctgacgc actgaatctg agaattgggt cattttgacg aatttctttt tgacgcggcg
     4381 accagttttt ggttgatgat atgagagaaa atactaaaat tgaccgaaca ctaggagaaa
     4441 atgccgtaac actatatgga agccaccgca ccagttgagg atagttttta tattgtcgcc
     4501 actgagtctt cggcggacca cgcgacccgg gtgctcaagg acggcgacac cttcgcggtt
     4561 tttgatcagc acggtgatat ccaggcggaa aaccggaccg gacaagaggg tgtttaccac
     4621 gaaggaacaa ggttcctctc acatttgctt ttcaagctgg ggaacgcccg tccgttcctg
     4681 ctcaattcca ccgttcagga agacaatctt agcttcgtcg tgaatttgac gaaccccgac
     4741 atttatcggt cgggacatct tctgttgcca cgtggaagtt tgcatatcac gcggagcaag
     4801 atgctgcggc aatcgaggtg tttcgagacg ttcagattca tcaactatgg tttatctccc
     4861 gtcgaggtgc ggttttcaat tcagtttggc gccgacttcg ccgatatctt tgaagttcgc
     4921 ggcatggcca ggcaagcgcg gggaagtttt cg
//
LOCUS       DASULZ010000260         4215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_640907, whole genome shotgun sequence.
ACCESSION   DASULZ010000260 DASULZ010000000
VERSION     DASULZ010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640907"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VFY96_05420"
     CDS             complement(<1..823)
                     /locus_tag="VFY96_05420"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX5605933.1"
                     /translation="MPLVPKSLFASREKVGIFISYRRADTAGHAGRLVDQLKSRFGDQ
                     LFFDVDSIKPGANFHQVIEETFTKCGAVIVLIGKRWLERDTGTPRFGEEQDVITQEIG
                     LAMASKLPILPVLVDGASMPAQSQLPPSLASLSSLNAIDLRHTSFDRDLQAVNDHLVE
                     ILGGAQETVIEKFALKLYGPFLGNSIAKIQGGIVLFAAMGALWALGELAGAAFLSWRS
                     GFQTLMEPSIIDAETTRLQAVWTAAFGALIFAFFGRRSIRWWRHATILVWISLAEI"
     gene            1054..1446
                     /locus_tag="VFY96_05425"
     CDS             1054..1446
                     /locus_tag="VFY96_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HEX5605934.1"
                     /translation="MAFFSKILEKLGFGSSATAAPQATTAAPASPAATPKESAPKAVA
                     VVDVVAQLEQRAAANPQKLNWRTSIVDLLKLLDIDSSFNARKELATELGCPADLMGDS
                     AKMNMWLHKTVLQRIADNGGNVPKELLD"
     gene            1484..2026
                     /locus_tag="VFY96_05430"
     CDS             1484..2026
                     /locus_tag="VFY96_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HEX5605935.1"
                     /translation="MQVTDILAQMGGLQSMARELGVSENQAASGTEALVPAILGGFKK
                     QAQSQPSGVEGLGDLLGQLGGGSLMDDVLSPQPTNVDRGNDVLGQIFGSKDVSRTVAQ
                     NVASQSGLEPSLLKKMLPMVAMLVAGYMAKQRSAAGPEQPSRAGGGLGDLLGGLLGGQ
                     ATGAGNPLDDVLRMAGKVFR"
     gene            complement(2421..3455)
                     /locus_tag="VFY96_05435"
     CDS             complement(2421..3455)
                     /locus_tag="VFY96_05435"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5605936.1"
                     /translation="MYRKPKSLLVSFIMGYLITGIAVAAGDEGFYKGKTLRLIVAFAP
                     GGGFDTYSRAIGRHMSKHIPGSPSIIVENMTGAGGFIHANYMYRQAKPDGLTLGNNIG
                     GLILQQIMGAKGADFDGKQFEFIGAPSIDNQVCVLTKASGITSVDQWFAAKQPVRLGG
                     VGAGGSASDVARTVQAALKLPIRVVEPYKGTAEIKLAAESGELDGGCWAWESMKTMWP
                     RALETGDVNVLLMVMPKKHPELPNVPLAIDYAKTEDARRILKYAVHDIATVTRIYFVP
                     PATPKDRVQLLRKAFGDTLKDPEFVADAKKSKLDIEAVAGEELQKLVQDLYKIDSSTL
                     ARLKEVLLPK"
     gene            complement(3544..>4215)
                     /locus_tag="VFY96_05440"
     CDS             complement(3544..>4215)
                     /locus_tag="VFY96_05440"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605937.1"
                     /translation="ILNRAVMSIYAKPEIRSLADLKGKVLAVTVPGATTDFAARVLLQ
                     QARLTPGKDVKLVYLKGMVEILTGINQGNAEAGIFTSPTTLKAEHAGLKELVNVTEQN
                     IPMIHAALASTKDYIKTQPDNVRRFLQAYLEGIKIARTDAEFAKQVIGKYTKTTDTAD
                     LENSYQTFLPAWEKLPLVPAAAVQTMLNFATHPKAKTAKPESLIDNSLLSELGKSGFV
                     DRLYK"
BASE COUNT          924 a         1206 c         1155 g          930 t
ORIGIN      
        1 gtatttctgc cagtgaaatc catacaagaa tcgtcgcgtg gcgccaccac cgtatcgatc
       61 ttcgcccaaa gaaagcgaaa atgagcgctc cgaaggcagc ggtccaaacc gcctggaggc
      121 gtgtcgtctc cgcgtctata atcgaaggtt ccatcagagt ttgaaacccg ctgcgccagg
      181 aaaggaaggc agcacctgcc aattcgccga gcgcccacag cgcgcccatc gcagcaaaca
      241 gtacgatccc cccttgaatc ttcgcaatcg agttgcccag aaagggaccg tagagcttca
      301 aagcaaactt ttcgattact gtctcttgcg cgccgccgag gatttcgacg agatgatcgt
      361 tgaccgcttg caggtctcgg tcaaaagagg tgtggcgaag atcgatggca tttaggctgg
      421 agaggctcgc gagcgaaggc ggcagttgag attgtgccgg catggatgcg ccgtcgacca
      481 gcaccggcaa aatcggcagc ttcgatgcca tcgccaatcc aatctcttgc gtgatcacgt
      541 cctgctcttc gccgaagcgc ggcgttcccg tatcccgttc cagccatctc ttgccgatca
      601 gtacaatgac ggccccgcac tttgtgaaag tttcttcgat gacctggtga aaattcgctc
      661 ccggctttat cgaatccaca tcgaaaaaca gttgatcgcc gaaacgtgac ttcagctgat
      721 caactaagcg gcccgcatgc cccgccgtat cggcgcggcg atagctgatg aagatcccaa
      781 ccttttcgcg agaggcaaac agcgatttcg gaaccagagg catttctaaa atggcgtgta
      841 ttcaatctat atgcgaaatt tcacactgcg ctgtcaacga ttattttatt ggctgcaacc
      901 gcaggtgcgc gtctgaacaa gacccggaaa aaaataatgc gcgctcgacg tataagcctt
      961 tctcgctgcg tttgtcggtg gtatgcgtgt tatggtgcga tgtgatgtgt gagttgcagt
     1021 tcgctttatt gacgtccaaa aaaaggagat cccatggcat tcttcagcaa gatactcgag
     1081 aaactcggct tcggcagctc ggccactgct gcgcctcaag cgactacggc ggcgccggcg
     1141 agccccgctg cgacgccaaa ggaatccgcg cccaaggcgg tcgcggttgt cgatgtggtc
     1201 gcacaactcg agcaacgcgc cgcggccaat ccacaaaagc tcaactggcg gacttcgatc
     1261 gttgacctgt tgaagcttct cgacatcgac agcagcttca acgcacgtaa ggagctcgcc
     1321 accgagctgg gctgcccggc tgatctaatg ggagattcgg caaagatgaa tatgtggcta
     1381 cataagacgg tgctgcaacg catcgctgac aacggcggca atgtacccaa agaactgctc
     1441 gactgagcag cggcgccaac aaccaattaa ggagtccaca cacatgcagg tcacggacat
     1501 cctcgcccag atgggcggcc tccagtcgat ggcccgggag ctgggcgtca gcgagaacca
     1561 ggcggcgagc ggcacggagg cgctggtccc ggcgatcctc ggcggtttca aaaagcaggc
     1621 gcagtcacag ccgtcgggcg tcgaaggtct cggcgatttg ctcggacaac tcggtggcgg
     1681 cagtctcatg gatgacgtgc tttcgccaca gccgacaaac gtggatcgcg gcaatgatgt
     1741 tctcggacag attttcggct cgaaagacgt gagccgcacg gttgcccaaa acgtggcgtc
     1801 gcagtccggt ctcgagccgt cactacttaa aaagatgctg ccaatggtcg ccatgctcgt
     1861 agcaggttac atggcgaagc agcgaagcgc agccggtccg gagcagccgt cgcgcgccgg
     1921 tggcgggctc ggagacctgc tcggcggttt gctgggcgga caggcgactg gggctggcaa
     1981 cccgctcgac gacgtcctga ggatggccgg caaggttttc cgttaaccgt ggcagtgcga
     2041 gcccggttcc gttcaacgaa cttgactagg agaaatccaa aatgaggctc attcgcaccg
     2101 tcgtgctaac agtggtctcg tattagcggt ctctcaagca agggctgtag cgcaatctcg
     2161 agtgccaggg gcatccagcg gtaccgccgg cagcaagcct ttgacactac aatgcgacgc
     2221 ttacagaccc gctccgagtt gttcttaaac ccgcgacaac ggcgttgttg gtcttcgaca
     2281 tcgtcgatcc catttgcaag actcaacggg aatgcaccgg gatatggtgc ggcgtgcgat
     2341 cgaagtcatt tctgaccgct gccgacataa attttgccgc tgcatctcaa aacaacgcgt
     2401 cccgccggag acgcggtcct tcacttcggc aacagcacct ctttcagtct cgccaacgta
     2461 gacgaatcga ttttgtagag gtcctgcaca agcttctgca actcttcgcc cgccacggct
     2521 tcaatgtcca gcttcgactt tttcgcgtcg gcgacaaact ccggatcctt aagcgtgtcg
     2581 ccaaaagctt tgcgcaatag ttgtacgcgg tctttcggcg ttgcgggcgg cacgaaatag
     2641 attcgcgtga ccgtggcgat atcatgaacg gcgtacttca aaatgcggcg cgcgtcttca
     2701 gttttggcgt aatcgatagc cagcggaaca ttgggaagct caggatgttt ttttggcatg
     2761 accatgagaa gaacgttcac gtccccggtt tccagcgctc tcggccacat cgtcttcatt
     2821 gactcccaag cccaacagcc tccgtccagc tccccgcttt cggcagccaa tttgatttca
     2881 gcggtgcctt tatatggttc gacgactcgg atagggagtt tcagtgccgc ttggacggtc
     2941 ctcgcgacat cggaagccga gccacccgcg ccaactcctc ccagcctcac aggctgcttc
     3001 gccgcgaacc attgatccac gctggtgatg ccgctggcct tggtcaacac gcaaacctga
     3061 ttgtcgatcg agggcgcccc gataaactcg aattgtttgc cgtcgaagtc ggctcccttt
     3121 gcgcccataa tctgctgcag gatcaggccg ccgatgttat tgccgagcgt gagaccgtcg
     3181 ggcttcgcct gtcggtacat gtaattggca tgaataaaac cacctgcccc ggtcatattc
     3241 tcaactataa tcgaaggact gcccggaatg tgtttgctca tgtggcggcc tatcgcccga
     3301 ctgtatgtgt caaaaccccc gccaggcgca aaagctacaa tcaaacgaag cgtctttcct
     3361 ttgtagaatc cttcgtcacc agccgccacc gcaattcccg tgatgaggta acccataata
     3421 aagctgacca atagggattt cggttttcgg tacataagtc ttcctttctg gtggctttca
     3481 gtccgaataa gttcgactta ggtaagtccg acatcgagct cgcggcgttt gaagcgacag
     3541 cggctactta taaagtcggt cgacaaaacc ggacttgccg agttcgctca gtaacgaatt
     3601 atcgatcaag ctttcaggtt ttgcggtttt ggccttggga tgggtcgcaa aattgagcat
     3661 ggtttgcacc gccgcagccg ggaccagggg caatttttcc catgcgggaa ggaaggtctg
     3721 ataggaattt tcgaggtcgg cggtgtctgt ggtcttggta tacttgccga tgacttgctt
     3781 ggcgaattcg gcgtcggtgc gggcgatttt aatgccttca agataagcct gtaagaaccg
     3841 ccgcacattg tcaggctgtg tttttatgta gtccttcgtg ctggcgagcg cggcgtgaat
     3901 catcggaata ttttgctcgg tcacattgac caattccttc aaccctgcgt gttccgcttt
     3961 caacgtcgtc ggcgatgtaa aaatgccggc ctccgcgttt ccctgattga tccccgtaag
     4021 aatctccacc atacctttga gatagaccag tttcacatcc tttcccggcg tcaaccgcgc
     4081 ctgctggagc aaaacccgcg ctgcaaaatc cgtcgtcgcg ccgggcacgg tcacggccaa
     4141 cactttgccc ttgagatcgg ctaacgaacg aatttccggc ttggcgtaga tcgacatcac
     4201 ggcacgattg agaat
//
LOCUS       DASULZ010000261         3085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1452274, whole genome shotgun sequence.
ACCESSION   DASULZ010000261 DASULZ010000000
VERSION     DASULZ010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1452274"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VFY96_05445"
     CDS             complement(<1..330)
                     /locus_tag="VFY96_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005829351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX5605938.1"
                     /translation="MLLAAGQGTRLRPLTNRKPKALVPVAGRPMIEYPLLLLRHYGIR
                     EIMINLHHFGEQIENQLGTGQQLGLEISYSEETELLDTGGGLLKAKPFLQDGTFIVIN
                     TDALIDLD"
     gene            complement(336..1412)
                     /locus_tag="VFY96_05450"
     CDS             complement(336..1412)
                     /locus_tag="VFY96_05450"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX5605939.1"
                     /translation="MVIDRLQQLISEKIGLIFGAGVTLTSLAPLAGDASSRRYYRAQL
                     RGVGAPGTAIVMDLPAGSTLPLSSEELAVFKERPKELPFLNLHGFFSRIGVHVPKLYG
                     HWENQGIILLEDLGDTALWDRVQNLPDEQIIAWYQQAIDELLHLQIVGTAQRDDACIA
                     FQQRFDFKLYMWEFDHFIEWGLEKRPGAKIDGAAIDQLRRWFTGISETLENQVPCLNH
                     RDYHSWNLMVKNESVRVIDFQDALLAPPQYDLASLLNDRITDEIVTPPIEERLLGYYI
                     ESSRTLGRMLEPNEFIEIYRLSAIQRDLKVVGRFYYLDLMKGKPGYRKFIPPTVRRLA
                     RNLANTPQARPILPLLLEQFEAMQ"
     gene            complement(1482..1697)
                     /locus_tag="VFY96_05455"
     CDS             complement(1482..1697)
                     /locus_tag="VFY96_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_258239539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(1659..1661),aa:Sec)
                     /transl_table=11
                     /product="UXX-star (seleno)protein family 1"
                     /protein_id="HEX5605940.1"
                     /translation="MAEKVIIFGKDLUPYTAGAREAFERRKVPHQYINVLEDEQGLER
                     MLKLSNGRRQVPVILEAGKISIGFGGS"
     gene            1941..2774
                     /gene="rsmI"
                     /locus_tag="VFY96_05460"
     CDS             1941..2774
                     /gene="rsmI"
                     /locus_tag="VFY96_05460"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017746588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX5605941.1"
                     /translation="MSGALYIVPTPIGNLEDVTFRAVRVLKEVDLIAAEDTRHSQVLL
                     NQYGIRTPVTSYHEHNERSKARELVEQLRQGTSIALLTDAGTPLISDPGYRLVIEAIG
                     AGVQIIPLPGPSAVIAALSAAGLPTNRFGFEGFLPAKKNERRSALEALKEDTRTLIFY
                     EAPHRLKETLADMAEIFGNREVAIGREISKVHEEFLRGTIREILATLEQQNVRGEITL
                     VLQGATSGAPVSEEGVISEIRQLAENGMRVKEISELVGAHHGISKREVYRLALRVKNL
                     K"
     gene            complement(2816..>3085)
                     /locus_tag="VFY96_05465"
     CDS             complement(2816..>3085)
                     /locus_tag="VFY96_05465"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010995051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX5605942.1"
                     /translation="IRARVKIAGRVQGVYFRASACQEAQSLGVSGWVRNCSDGSVELV
                     AESTREKLDQLIAWCHCGPAGARVAEVTVDWEEPEHGYRGFVIKR"
BASE COUNT          704 a          838 c          871 g          672 t
ORIGIN      
        1 gtcgaggtcg atcagcgcat ccgtattgat cacgataaaa gttccatcct gcaaaaatgg
       61 ttttgccttg agcagccctc cgccagtgtc cagcaactcg gtttcttcgg aatagctgat
      121 ttctaaacca agctgttgcc cggtcccaag ctgattttcg atctgctcgc caaaatggtg
      181 caagttgatc atgatttcgc gtatcccgta gtggcgcagc aggagcagcg gatactcgat
      241 catcggccgc cccgcaacag ggaccagggc tttaggcttg cgattggtca gagggcgaag
      301 acgggttcct tggccggcgg ccaacagcat cgctttcact gcatcgcctc gaactgttca
      361 aggagcaggg gcaagatcgg tcgggcctgc ggtgtattcg ccagattccg cgctaggcgt
      421 cgaaccgtcg gcgggatgaa tttcctgtaa ccgggtttgc ccttcatcag atcgagataa
      481 taaaaccgac cgacaacttt caggtcgcgt tgaatcgccg acagccggta aatctcgatg
      541 aactcattgg gttctagcat gcgcccgagg gttcggctcg attcgatata atagccgagc
      601 agccgttctt cgataggtgg cgtgacaatt tcatcggtga tccggtcgtt gagcagagac
      661 gcgagatcgt attgcggagg cgcgagaagt gcgtcctgaa aatcgatcac acggacggat
      721 tcatttttca ccatcaggtt ccagctgtga tagtcgcgat ggttcaggca aggaacttgg
      781 ttctccaaag tttccgagat tccggtgaac catcttcgca attgatcgat ggcggctccg
      841 tcgattttgg cacccggacg tttttccaaa ccccactcaa taaagtggtc gaactcccac
      901 atgtaaagct taaaatcgaa acgctgttga aaagcgatgc aggcatcgtc tcgctgagca
      961 gtaccaacga tttgcaaatg cagcagctca tcgatcgcct gctgatacca ggctatgatt
     1021 tgctcgtccg gcaagttctg cacccggtcc cataacgcag tgtcgcctag gtcctcgagc
     1081 aatataatgc cttgattttc ccaatggccg taaagcttcg ggacatggac tccgatccgg
     1141 ctgaaaaagc cgtgcaagtt caagaaagga agttctttcg ggcgttcttt gaatacggcg
     1201 agctcctcgc tggaaagggg cagcgtgctg ccggcgggta ggtccatgac aatcgcggtc
     1261 ccgggcgcac cgacaccgcg caactgagcg cgatagtacc ggcggctgga agcgtcacca
     1321 gcaagagggg ccagagacgt caacgttacg ccggctccaa aaatcaggcc gattttttcg
     1381 ctgatcagct gctgaagacg atcaatcacc attcgccttg agtggagtgg atgagaccag
     1441 ttagctaatg aaggcaccgg cgccgccggt agctaaactt ctcatgaacc gccgaagccg
     1501 atgctgatct ttccggcttc gagaatgaca ggcacttgcc gtcgtccgtt cgacagcttg
     1561 agcatccgct cgagtccctg ctcgtcctcg agcacgttga tatactggtg tggaactttg
     1621 cgccgctcga aagcctcccg ggctccggcc gtgtagggtc aaaggtcttt gccgaaaata
     1681 atgactttct ctgccatgac gctgcccgcg gattgcttta taaaaaataa gcgccaacgg
     1741 gtgaaattgc aagggaagtt taccgcatgc tagtttgcga acgagcatgg aatccctcga
     1801 tacgccctcc gggctactcg ggatgatcga tcggcgggga gggatcgcgg attctgaggg
     1861 gtcggacgag cgtagagaac gggatgagag cgtccgatcc tcgattctcg atcgtctgtt
     1921 ctcgtagcca agtatcggaa atgtccggcg ctctatatat tgtccctaca cccattggca
     1981 atctcgaaga cgtaaccttt cgggcggttc gcgtgctgaa ggaagtcgat ctgatcgcgg
     2041 ccgaagacac ccgtcatagc caggtgctcc tcaaccaata cggcattcgc accccggtta
     2101 ccagctatca tgagcataat gagcggagta aagcgcgcga gttggtcgag cagctccgac
     2161 aaggcacaag cattgcgcta ctcacggatg cgggcacacc gctgatctcc gatccgggtt
     2221 atcggctcgt catcgaagcg attggcgctg gtgtgcagat cattcctctt ccggggccgt
     2281 ccgccgtaat cgctgcgtta agcgctgcag ggttgcctac gaatcgattc ggcttcgagg
     2341 gatttttgcc ggcgaaaaaa aacgagcgca ggagcgctct ggaggcgctt aaagaggata
     2401 ccagaactct gattttctat gaggcgccgc accggctcaa ggaaacgctt gccgacatgg
     2461 cggagatttt cgggaaccgt gaagtagcaa tcggccgcga aattagcaaa gtccatgagg
     2521 agttcttacg gggaacgatt cgcgaaattc tcgccacgct tgagcagcag aacgtcaggg
     2581 gtgagatcac gctggtcctg cagggtgcta ccagcggagc tcccgtttcg gaagaaggag
     2641 tgatcagcga gatacgccag ttagctgaaa acggtatgcg agtaaaggag atttcagaat
     2701 tggtgggcgc tcatcatggg atctcgaaac gagaggtcta ccggcttgcg cttcgagtaa
     2761 aaaatttgaa gtgatgcgac ggggttaact aaacgggcgc agcatgctgc gccccctagc
     2821 gcttgataac aaacccacga taaccgtgct ccggctcctc ccaatccaca gtcacctcgg
     2881 cgacgcgggc gccggcgggc ccgcaatgac accaggcgat taattgatcg agtttttccc
     2941 tcgtgctctc ggcgactagc tcgaccgagc cgtcagaaca gtttcttacc catccgctaa
     3001 cccccaagct ttgagcttcc tggcaggcgg aagcgcgaaa atagacacct tgcactctac
     3061 ccgcgatttt cacccgagcg cggat
//
LOCUS       DASULZ010000262        14802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_438576, whole genome shotgun sequence.
ACCESSION   DASULZ010000262 DASULZ010000000
VERSION     DASULZ010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 14802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14802
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438576"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..1058
                     /gene="corA"
                     /locus_tag="VFY96_05470"
     CDS             93..1058
                     /gene="corA"
                     /locus_tag="VFY96_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEX5605943.1"
                     /translation="MLINCAAYQDGKKLADIPVDQIHLFISRPGCFVWVALKDPEAAE
                     LKAMQAEFGLHDLAVEDALKGHQRPKIEEYGQSLFVVLHMIENGGEDLSVGEVCIFVG
                     SNYVLSVRQRAQQGFVEVRKRCEQEPELLQHGAAYVLYALMDAVVDRYFPVLDAISTE
                     LERVEQRIFATQTERASIEALYSIKQKLMTLKHATEPLLEAVGRLHGGRVPQLFHGLQ
                     DYFRDISDHLVRLNQSIDSLRDMVSTAISVSLSLITLQENEVTKRLAAYGALVAVPTL
                     IAGIYGMNFEHMPEIRWEHGYLVSLITMALIDGYLFYRFRKAKWL"
     gene            1214..2035
                     /locus_tag="VFY96_05475"
     CDS             1214..2035
                     /locus_tag="VFY96_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5605944.1"
                     /translation="MTEMTTILQRVDEAAGVAWLTFNRPEKKNAMSHTFMAELIAALK
                     DLADNDKIKCVVTMGAGDSYSSGLDLYDLRESWKRKRAWDHAGTTYEIVRVLRNMPQV
                     TVAAVRGWCLGGGLALINGHDLCIAGESAKFGMPEVIRGSYGAVATSTLFHSGIPVKK
                     AFYIQLTGRNLTAAEAERIGLVSEVVPDDQLIASAEQLAKELGSRNSVTLESAKIAAY
                     MQKDMDFLTALRTDDLVSHRMRYYTNPLSDVEGYLHSQKGGGTTRYVKPEDRKKS"
     gene            2181..3311
                     /locus_tag="VFY96_05480"
     CDS             2181..3311
                     /locus_tag="VFY96_05480"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEX5605945.1"
                     /translation="MNLHTPLCDLLNIQVPIIQAGMGWDKEGMTTPPELVAAVSNAGG
                     LGCIGGSSIRPEVIRERIRAVRRLTDKPFGVDITLPKMEDVKIPDPDEVRKEIEEKYP
                     KHAKFTDELIPKLGLAPQPPDRKSWVKTPAATREQLKVILEENVPILIIGLGDTAEVV
                     PLAHERGIKVMALAGAVKHALSHARKGADVIIAQGYEAGGHTGTIGNFPLIPQIVDAV
                     KPKPVVAAGGISDGRGVVAALALGAVGVWCGTTFLVSKECGINPVYKKQILTGSSEDF
                     VRTAYTSGHYARHYRSPVIKAWLDSGLDAMPTPYQGNAMDEIRRAAEAAERVDVLHVP
                     GGQVAGMLSEEKSARPAKEIVERMVSEAAQILNDMRSRYVGA"
     gene            3452..5887
                     /locus_tag="VFY96_05485"
     CDS             3452..5887
                     /locus_tag="VFY96_05485"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5605946.1"
                     /translation="MSGEFLKGFRALDLTDLSGQLCGRVLADLGMEVIKIEPPGGDPV
                     RNLAPFIISADGKRLSTVFAHLNAGKASKVLDLNAEAGRAQFRELVKDADVVIESFQP
                     GDLDGKGLGYQDLTTINRRIVMASITGFGQTGPKKNLACNDLVALAESGFLYISGDPS
                     LPPAKPPETQAYYFASLFATAGLLAALYRRERTGQGDHVDASMQEALATQEHIIRLWA
                     NEQQIAKRAGSQHGSVAPAKIFSCKDGFVYLYVTRQHWKLFLSLWKEHPTVFDAPDWL
                     NNLYRRAHADELNRAVEAFLNGFTMAEITEFLQAKGIPCVPVNTPLGFTNDEQVRERG
                     FMAPVEHAGFGKTKQPAIPFVIDGARPPVGSVPVLDSWQPSAAIPNERQGSKISPFDR
                     NDRIPGNDEGRGGETLSNSDGPLHGMRIVSFDHVLAGPYGTTILAELGADVIKVESSK
                     GGMDPFRFFGTGEDPNLSPRFLEFNRNKRSFTVNLKHPKGQTVLHDLVAKADAVLDNY
                     SVDVVERIGLAYDQLRKVNRDIINLRMPGLGTTGPKRHFSTVGVNITAFTGLTHLWNH
                     PGVTHPPIGSQTVFPDYVSGTLCAIIIISGVIYRERQKRGAFIDLGQSEATAFMIGAH
                     LMEAAASEKNPEPIGNLSLSVAPHGCYPCLGEDRWCVIAAESEQQWAALAKIIGSGTN
                     DDPRFATNAERLKNRDALDAMIGSWTKTKDAFALRDQLQGAGIPCGVVQTGEDLVNDP
                     HLQQRGFIVAVDNPRLGRVVLPNFPLQFTNAKLTRRWEFPVLGRDTETVLRDVVGYDE
                     ATIAAHRRDGVLE"
     gene            6118..6384
                     /locus_tag="VFY96_05490"
     CDS             6118..6384
                     /locus_tag="VFY96_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605947.1"
                     /translation="MRSLLGALLFCLMASTSVAQTKSIAELANYRGADREEMLKAGAK
                     KEGKMVWYTSLTAHRDIANIFEAKYPGVKVETYRAGPNDLTRGS"
     gene            6417..7148
                     /locus_tag="VFY96_05495"
     CDS             6417..7148
                     /locus_tag="VFY96_05495"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5605948.1"
                     /translation="MIETTPSTLMMMRENKLLMPYYSPHLTQFPEEAKEEADKGRVLW
                     TTDRESIVGLGYNHNLIRAADVPKSFAELARPENKGKIAVSGDTTGVRFLGAMIRAKG
                     EEYVKQLRALDIKMHMISGGAMHELMAAGEMPMSISIFRNHVLAAKPKGAPTEWVALD
                     LSPTNAGGVALPAGANNPYAALLFADFLLSPEGQKIFEEKFRFATSTKNYGFKRWYPE
                     KGMTLEQYEKAEDHWKKLLRDLSRR"
     gene            complement(7344..7841)
                     /locus_tag="VFY96_05500"
     CDS             complement(7344..7841)
                     /locus_tag="VFY96_05500"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEX5605949.1"
                     /translation="MAIDQRRVDRRAVQETDRLWLARFLADLLDQRFTIPGTSIRIGL
                     DPIVGLIPGVGDLLANLTGSLIVVIAAQLGVPKIVLARMGINVAINTLLGAVPIFGDV
                     ISIWYRSNVKNVELLERYVGRQSKRVESKDWLFVIALIVGLLLLLGGVVVFGIWVLKQ
                     IWQAV"
     gene            complement(7925..8923)
                     /gene="ligD"
                     /locus_tag="VFY96_05505"
     CDS             complement(7925..8923)
                     /gene="ligD"
                     /locus_tag="VFY96_05505"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX5605950.1"
                     /translation="MRQPPGKAVRRPVKSRSSQNRSVRVRGGQDTDAFETNGKLVHLT
                     NLRKIFWPDLGITKRDLLEYYIDIAPVLLPHLADRAMVMKRYPNGIGGDFFFMKRAPS
                     PRPEWIETCAIEHASGNIIDFPIIADLAALVWVINLGCIDLNPWYGRCDDVDRPDYLH
                     FDLDPVKDASWSQMLEAAALVHEALTGLGMPALIKTTGSRGFHVYVPIVRGPTQKEVW
                     RFAKEFAMSLAALRPDLITAEYRIAKRPRQRVLVDYNQNAWGQTLASIYSVRPQPKAT
                     VSTPITWNELEQSVRIEDFRMDNVPQRIKKVGDLWAPLLAQKGRVKLEKFYAAADD"
     gene            complement(9033..9848)
                     /locus_tag="VFY96_05510"
     CDS             complement(9033..9848)
                     /locus_tag="VFY96_05510"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5605951.1"
                     /translation="MKLFSTSEVAKILNLPDSRIRTFVRAGFLAPGRDDTKSLQFTFQ
                     DLLFLKTAKSLLASRVPAKRIVRILSSLKRQLPDEQHLSSVKIYADGRRVVVWDGHAR
                     WQPDSGQFLFNFDARSVIAAIKLPPSKRAKKDLGADYWFKLGTELEATSAEEAQGAYR
                     RALEIDPNIADAHLNLGKLCHEDGQLDQAETHYRAAARLASREPAAYFNLGVLLEDLK
                     RPREAVECYKESLSRDPDFADAHYNLGLLLENLGNKTEAFTHLRAARKLYIGK"
     gene            complement(9845..10699)
                     /locus_tag="VFY96_05515"
     CDS             complement(9845..10699)
                     /locus_tag="VFY96_05515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX5605952.1"
                     /translation="MPPRSIGSGAISFGLVSIPVKLYVATSSQAPSFHLLHAKCGNRI
                     RQQRVCPVDNEVVERDQLVKGYEFAKDQYVRVTDDELKALEGEASEAIEISEFVPLSK
                     VDPIYFERSYYLGPDKGGEKAYRLLTDTMTQVGKVALAKFVMRGKENLVIVRAAQKGL
                     MMHTMYFANEVRNFDEIPKGESARITGAETSLAIRLIDELSNDEFEPEKYHDEYSDRV
                     LKLVNEKAEGKEITLAQPVAQRGQVIDLMAALKESLEKTGPRQKRPAVSAKQAEPEKP
                     RKKASSKK"
     gene            10858..11676
                     /locus_tag="VFY96_05520"
     CDS             10858..11676
                     /locus_tag="VFY96_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5605953.1"
                     /translation="MATYQTLLVDKQDGITTITLNRPEKRNAMSPQLHREMYDCLTDL
                     KYDQETRVIVITGAGESFCAGQDLKQYFIEMDSQPDRVRDEVREKSRQWRNEMLRKMP
                     QPVIAKINGWVFGGGFTVVTGSDIAIAADDVQFGLSEVNFGHFPGGEVTIVLTEHLQP
                     KHGLYYALTGKTMNAKEAERIGLITKAVPRADLDREVSELANNLKEKAPSALNAVKEA
                     WYYTFYSNPDVAYEISGLISDKAKAAIGGRPGLQQFARKEYRPGLGSYKFEEKK"
     gene            11755..12909
                     /locus_tag="VFY96_05525"
     CDS             11755..12909
                     /locus_tag="VFY96_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5605954.1"
                     /translation="MDFDLPEELQMLKDTVRKFVDRELIPIEMHTIENMELKPDIRER
                     LEKKTKEMGLWMFDVPEEYGGGGLGSLAQVLVWEELARTVALPSRGQGIFGPEIRPIL
                     YALNAEQKKRFLDPIRNGEKKLCFMQTEPDAGSDPASMKTRAVRQGDYYIVNGTKRFI
                     TGAGDADFGQLMAVTDPAKGAHGGISCFMVDMKTPGLTLVTRYKTIMGEEPWHITFDD
                     MKVPAENLVGKEGEGFKLAQKWLGIGRLKHGARALGVAERCIEMGARYSKERVTFGKP
                     LSERQGITFKLADSYVELHAARLMVYQAAWKNDQKQDIRNEAYMAKLFADEMSFRVVD
                     RVLQIHGGIGLTLDLPLAKWFVDQRSRMITEGASEVMRMVIAREVLRRYN"
     gene            complement(13006..13395)
                     /locus_tag="VFY96_05530"
     CDS             complement(13006..13395)
                     /locus_tag="VFY96_05530"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5605955.1"
                     /translation="MFDIKGLRTFAMSAKNLDKSVEFYTKTLGGQIVKSVEPTEEQKK
                     AGQVKEVDVRLGNFEVHLFDASIKPREVDPHHTLNIPWQEKEQALAALQNIGANIEKV
                     RPHRDGENYSINVFDPDGNRWELSFTK"
     gene            complement(13562..14587)
                     /locus_tag="VFY96_05535"
     CDS             complement(13562..14587)
                     /locus_tag="VFY96_05535"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5605956.1"
                     /translation="MQRHWFVLIWLFFTPAAWAQTPFYQGKTITVVAGVSAGSAYDLY
                     ARLMAQFMGKHIAGNPSFIVQNMTGAGSIIGANYVYSVSKPDGLTIGAIQPSIYFNQL
                     MKQKEVKYDWAKFGWIGSSDKSDYLLYMRADLPYKTLADVRRAKEPPKCGSTGAGTSG
                     SYMPKLLEETLGTKFTIVAGYQGGGEIDLAVERGELHCRAFTVQAYHSREPYFTWRKN
                     GFARILMQTGQTRDARLPDVPTLGELMDQFKTPERERRLVPLVMAATDFGRPIVAPPG
                     IPADRLKILREAFLKTMNDPELLAEAKRKNFDITPSTGEELESLAKLVMNQPEEVVAR
                     VKILMEQ"
     gene            complement(14613..>14802)
                     /locus_tag="VFY96_05540"
     CDS             complement(14613..>14802)
                     /locus_tag="VFY96_05540"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5605957.1"
                     /translation="DEDIPYLAEFIGADHLMIGSDYGHNDPAEEKALVQTIRSREDLP
                     PDLANKILTDNPKRFYSL"
BASE COUNT         3409 a         3948 c         4246 g         3199 t
ORIGIN      
        1 tatcggaata gcgggcagag agatatggtc gacgtggcgc agattcgaga gcgttaaact
       61 tccgcacaag tctccactga aggggtagtc ggttgcttat caattgcgcc gcttaccagg
      121 acggcaagaa actcgctgac attcctgtcg atcagattca tctgttcatc agcagaccgg
      181 gttgctttgt ctgggtagcg ctcaaggatc ccgaggccgc cgagctgaaa gcgatgcaag
      241 cggagttcgg cttgcacgac ctagcggtcg aagacgcgct caaaggccat caacgaccaa
      301 agatcgagga gtacggccag tccttgtttg tcgtgctgca catgatcgag aacggcggtg
      361 aagatctcag cgtcggcgaa gtgtgtattt tcgtcggcag caattatgtt ctgtcggtac
      421 ggcagcgcgc gcagcaagga tttgtcgaag tccgcaaacg atgcgaacaa gaaccggaat
      481 tgctgcaaca tggagccgcg tacgttctct acgctctgat ggacgcggtg gtcgaccgct
      541 acttcccagt gctcgatgcg atctccacgg agctcgagcg agtcgagcaa cgaattttcg
      601 caactcaaac cgagcgggcg agcattgagg cgctttatag cattaagcaa aaactcatga
      661 ccttgaaaca cgcgaccgag cctctgttgg aagcggtcgg caggcttcac ggcggccgcg
      721 taccgcagct ctttcacggc ttgcaagact attttcgcga tatttcagat catctggtgc
      781 ggctgaacca gtccatcgac agcctgcgtg atatggtaag caccgcgatc tcggtcagtc
      841 tgtctttaat tacgttgcaa gaaaacgaag tgaccaaacg gcttgccgca tacggcgcgc
      901 tagtcgctgt gccgaccctg attgccggta tttacgggat gaacttcgag cacatgcccg
      961 agatccggtg ggagcacggc tatctcgttt ctttgatcac gatggcgctc atcgacggct
     1021 acttgtttta tcgattccgc aaggccaagt ggctctagcg ctcgtgattg atgtttaagc
     1081 gggcgaacgc cgtccgcccc tacgaaaatc ttggatgtgt gtacgggtag gaaactgtct
     1141 cgggatttga tgatgcacct cgacttctga tactcatcag agataatttt ctgatccgaa
     1201 tgcgaggaaa tttatgactg aaatgacgac aattctgcag cgcgtcgacg aagcagcggg
     1261 tgtagcgtgg ttgacgttca atcgcccgga gaaaaaaaac gccatgagcc acacattcat
     1321 ggccgaatta atcgccgcgc tgaaagattt ggcggataac gataagatca aatgcgtcgt
     1381 gacgatgggc gccggcgatt cgtattcgtc aggtctcgat ctctacgatc tgcgggaatc
     1441 ctggaaacgc aaacgcgcct gggatcatgc gggaaccacg tatgaaatcg tgcgggtgct
     1501 gcgcaatatg ccgcaggtga ccgtcgctgc cgtgagggga tggtgcctcg gtgggggtct
     1561 cgccctgatc aacggtcacg atctctgcat cgcaggcgag tcggcgaagt ttggcatgcc
     1621 cgaggtgatt cgcggcagct acggcgcggt ggcgacgtcg acgctctttc atagcggtat
     1681 tcctgtgaag aaagcattct atattcagct caccggaagg aatttgacgg cagccgaagc
     1741 ggagcgaatc ggcttggttt ccgaagtcgt gcccgatgat cagttgattg cgagcgccga
     1801 gcaactggct aaagagctcg gcagccgcaa cagcgtcacg ttggaaagcg caaaaatcgc
     1861 cgcctacatg caaaaagaca tggattttct gaccgcgctt aggaccgatg atctggtgtc
     1921 gcaccggatg cgctactaca ccaatccgct gagcgacgtg gaggggtacc tgcactcaca
     1981 aaaaggtggg ggcacgaccc ggtacgtaaa gccggaggat cgaaagaaat cgtgaacatc
     2041 tgtaaaacag gattcttcgc tttgctcaga atgatattcg accgcagtgt cgttctcatg
     2101 tatcaccctg agcctgcgcg aagggtctcg tcactcgaac cgcgggcgca gcatgctgcg
     2161 cccctacgac cggagacgat atgaatttgc acactcccct ttgcgatttg ctgaacatcc
     2221 aggtgccgat cattcaggcg ggcatgggct gggacaagga aggcatgacc acgccgccgg
     2281 aactggttgc ggccgtttct aacgccggcg ggctcggctg catcggcggc agctcaattc
     2341 ggccggaagt gattcgcgaa cggattcgag ctgtgcggcg cttgaccgac aagccgttcg
     2401 gcgtcgatat cacgctgccg aaaatggaag acgtcaagat tcccgatccg gacgaggtgc
     2461 gcaaagaaat cgaggaaaaa tatcccaaac acgcaaagtt caccgatgag ctgatcccaa
     2521 aactcggcct cgcgccgcaa ccgccggatc gcaagagctg ggttaaaaca ccggccgcca
     2581 cacgcgagca actcaaagtc attttggaag agaacgtgcc gattctaatc atcggtctcg
     2641 gtgacacagc cgaagtcgtg ccgctcgccc atgagcgcgg catcaaagtg atggcacttg
     2701 ccggcgcggt caagcacgca ctcagccacg cacgcaaagg cgcagacgtg atcatcgcgc
     2761 agggttacga ggccggcggg cacacaggga cgatcggaaa ttttcccttg attccgcaga
     2821 ttgtcgatgc cgtgaagccg aagccggtgg ttgctgccgg cggcatttcc gacggacgcg
     2881 gtgttgtcgc ggcgctcgcg ctcggtgccg ttggggtttg gtgcggcacg acgttcttgg
     2941 tcagcaaaga gtgcggcatc aacccggttt acaaaaaaca aatcctcacc ggcagttcgg
     3001 aggattttgt ccgtacggca tataccagcg gacattacgc gcggcattat cgcagtccgg
     3061 tgatcaaagc ctggcttgac tcggggctgg acgcgatgcc gacgccgtat cagggcaatg
     3121 cgatggatga gattcggcgc gcggccgaag cggccgagcg cgtcgatgta ctgcacgtgc
     3181 cgggcggtca agtggcgggg atgctcagcg aggagaaaag cgccaggccg gcaaaagaaa
     3241 tcgtcgaacg gatggtcagc gaagcagcgc aaattctaaa cgatatgcgc agccggtatg
     3301 tgggagcgta gatagtcgag aaagattgct cgcttcgctc ggaatgacaa acagcatttg
     3361 tcatctcgaa ccattcggca gactcgcggt aaactccgtg agagatcttt ctcaatgcga
     3421 tcatccctga agtacggtct aaggaagaac tatgagtgga gaatttctca aaggattcag
     3481 agcgctggac ttgaccgatt tgagcggcca gctttgcggc cgcgtgctcg ccgatctcgg
     3541 catggaagtc atcaaaatcg agccgccggg cggcgatcct gtccgtaacc tggcgccatt
     3601 cattatctcc gccgacggca aacggttgag cacggtattc gcccatctca acgcagggaa
     3661 agcgagtaag gttctggatc tgaatgccga agcagggcgc gcgcagttcc gtgagttggt
     3721 gaaagacgcc gacgtagtca tcgagagttt tcaaccgggt gatttggacg gtaaaggact
     3781 cggctatcaa gatctcacga cgatcaatcg caggattgtg atggcatcga tcaccgggtt
     3841 tggccagacc gggccgaaga aaaacttagc ttgcaacgat ctggtcgctc tggccgaaag
     3901 cggctttttg tatatctcgg gagatccgtc cttgccgccg gcaaagccgc ccgagaccca
     3961 ggcctattat ttcgccagct tgttcgccac agccggattg ctcgcggcgc tttaccgacg
     4021 cgagcgaaca ggccaaggcg atcatgtcga tgcgtcgatg caggaagctc tggcgacgca
     4081 ggaacatatc atccgcttgt gggccaacga acagcagatc gccaagcgcg ccggcagcca
     4141 acacggctca gtcgcgccgg cgaaaatatt ttcctgcaaa gacggttttg tctatcttta
     4201 tgtcacgcgc cagcactgga agcttttctt gtcgttatgg aaggagcatc caacggtttt
     4261 cgatgcgccc gactggctca acaaccttta ccggcgcgcc cacgccgatg agctcaaccg
     4321 cgccgttgaa gcgttcctga acgggttcac catggcggaa atcaccgagt tcctgcaagc
     4381 gaaaggcatt ccgtgcgtgc cggtgaacac accgctggga tttacgaacg acgaacaagt
     4441 gcgcgaacgc ggcttcatgg cgccggtaga gcacgccggg tttgggaaaa ccaaacagcc
     4501 ggcgattccg tttgtgatcg acggcgcacg gccgcctgtc ggaagcgtgc cggttctaga
     4561 tagctggcag ccttcggctg ccatcccgaa cgagcgtcag ggatctaaga tttctccctt
     4621 tgatcgaaat gacagaatcc ccgggaatga cgaaggccgg ggcggagaga ctctttcgaa
     4681 tagcgatggc ccgctgcatg ggatgcgcat cgtaagcttc gatcacgtgc ttgccggacc
     4741 ttatgggacg acaattttgg ccgaactggg tgccgatgtg atcaaagttg agtcgagcaa
     4801 gggcgggatg gacccgtttc ggtttttcgg cacgggagaa gatcccaatt tgtcgccgcg
     4861 atttttggaa tttaaccgga acaaacgatc tttcaccgtg aatctgaagc atcccaaagg
     4921 gcaaacggtg ctgcacgatc tcgtcgccaa ggcagacgcg gtgctggaca attacagcgt
     4981 cgatgtcgtg gaacggatcg ggttagcgta cgatcagctg cgcaaagtca accgtgatat
     5041 tatcaatctg cgcatgccgg ggctgggcac caccggaccg aagcggcatt tttccaccgt
     5101 cggcgtcaat atcaccgcgt tcaccggact gactcatctg tggaaccacc caggagtgac
     5161 gcatccgccg atcgggtcac agacggtttt ccccgattac gtatccggta ccttgtgcgc
     5221 gatcatcatt atttcgggcg tgatctatcg cgagcggcag aagcgcggcg cgttcatcga
     5281 tctcgggcaa tcggaagcga cggcctttat gattggcgcc catctcatgg aagccgcagc
     5341 aagcgaaaaa aatcccgagc cgatcggaaa cctatcgctg tccgtcgcgc cgcacggttg
     5401 ctacccctgc cttggggaag accgctggtg cgttatcgcc gcggaaagcg agcaacagtg
     5461 ggcggcgctg gcgaaaatca tcggttccgg caccaacgac gacccacggt ttgcgaccaa
     5521 tgccgagcgc ctaaaaaatc gcgacgcgct ggatgccatg atcgggagtt ggacgaaaac
     5581 caaagacgcg tttgcgctca gggatcaatt gcaaggcgcg ggaattcctt gcggcgtcgt
     5641 tcaaacaggc gaggacctgg tcaacgatcc gcacctgcag cagcgcggat tcatcgtcgc
     5701 tgtggataat cctcgtctcg gtcgcgtcgt gctaccgaat tttccgctgc aattcaccaa
     5761 cgccaagctg acccggcgct gggaattccc cgtactcgga agagacactg agacggtttt
     5821 acgcgatgtg gtgggttatg atgaggcgac gattgcggcg caccgacgag acggagtgtt
     5881 ggagtaaaat tggcgacggc ctgattggga tccgtcactc cgttttagat cggatacacg
     5941 gtcgatctgc agccgcgtat gcctcgatac acgctgcgcg ctactcggga actcgggtcg
     6001 tccgatagcc tgagtaggca gcgagcagcg ctgccgtaac gaagggcgaa cgctggacct
     6061 tttacggaac ctcttgttga gacgacaacc tgtatcaact ggttaggaga tacgagaatg
     6121 cgatcactct tgggtgcatt gctattttgt ttgatggcat cgacttccgt cgcgcagacg
     6181 aaatccattg ccgagcttgc caactaccgc ggggcggatc gcgaagagat gctcaaagcc
     6241 ggcgcgaaaa aagaaggtaa gatggtctgg tatacgagcc tcacggcgca tcgcgatatt
     6301 gccaatatct ttgaggcgaa atatcccggc gtcaaagtcg aaacctatcg cgccggtccc
     6361 aacgatctca cccgcggctc ctaagcgaag cgcagagtaa gcgcaacatc gccgatttga
     6421 tcgaaaccac gccatcgaca ttgatgatga tgcgcgaaaa caaactgctg atgccatact
     6481 attcaccgca tctcacgcag ttcccggaag aggcgaagga agaagccgac aagggccgcg
     6541 tgctttggac caccgatcga gaatcgattg tgggcctggg ttataaccac aacttgattc
     6601 gtgccgccga tgtgccgaaa agctttgccg aactggcgcg acccgaaaac aaaggcaaga
     6661 tcgcggtcag cggcgatacc accggcgtgc gttttctcgg cgcgatgatc agggccaaag
     6721 gtgaagagta cgtgaagcaa ctgcgcgcgc ttgacatcaa gatgcacatg atttccggcg
     6781 gcgccatgca cgagctcatg gcggcagggg aaatgcccat gtcgatttca attttccgta
     6841 atcacgtgct cgccgcgaaa ccgaagggcg cgccgacaga gtgggtggcg ctcgacttga
     6901 gcccgaccaa tgccggaggc gtggctttgc ccgccggcgc caataatcca tatgcggcgc
     6961 tgttatttgc cgattttctt ctcagccctg aagggcaaaa gatcttcgaa gaaaaattcc
     7021 gctttgccac ctcgaccaag aattacggct tcaagcgctg gtatcccgaa aagggaatga
     7081 cgttggaaca gtatgagaag gccgaggatc actggaaaaa gctgcttaga gacttgtcgc
     7141 gccggtagcg ttcgttcaaa attctgcggc gaacaaattc cttaagccac gctctggctg
     7201 gctgtcctcc gacttgtcat ttcgagcctg aggcgagaat ctatcttctt gtagacacaa
     7261 gatctctcgc tccgctcgag atgacaatca taagagattc gggacccttc gactatgttt
     7321 cgaagccggt gcagagcaac ttatcagacc gcctgccaaa tctgttttaa gacccagatg
     7381 ccaaacacga cgacgccgcc caagagcagc agcaacccga caatcagcgc gatcacgaac
     7441 agccaatctt tgctctcgac gcgcttcgat tgtctgccaa cataacgctc gagcaattcg
     7501 acatttttga cattgctgcg ataccagatc gaaatcacgt caccgaagat tggcacggcg
     7561 ccgaggagtg tattgatcgc tacgtttatt cccatacggg cgagaacgat tttgggaact
     7621 cccaattgcg ccgcgatcac gacgatgagc gatccagtga ggtttgccaa aagatcgccg
     7681 actcccggaa tcaagccgac gatcggatcg aggccgatgc ggatcgacgt gccaggaatg
     7741 gtaaagcgct gatccagcag gtcagcgaga aatctcgcga gccaaaggcg atccgtttcc
     7801 tgaactgcgc gtcgatccac tcggcgttga tcaatggcca tgggttttcc tcgggagttc
     7861 tactcgtgtc atcaggttgc gaattctttc gacggacaaa gcttccatcg gcagaatgcg
     7921 cggattaatc gtcagcagcg gcataaaatt tttctaattt cacccggcct ttttgcgcca
     7981 gcagcggcgc ccaaaggtca ccgacttttt tgatcctttg cgggacattg tccatgcgga
     8041 agtcttcgat gcgaacgctc tgttcgagct cattccaggt gatcggcgtt gacacggtgg
     8101 ccttcggctg cggccgcaca gagtagatcg aagccaaggt ttggccccag gcgttttgat
     8161 tatagtcgac cagaacgcgc tggcgcgggc gtttggcgat gcggtattcg gcggtaatta
     8221 aatccgggcg gagcgctgcc aagctcatcg cgaactcttt ggcaaatctc cagacttcct
     8281 tttgtgtcgg tcctcggaca atcggcacgt agacgtgaaa gccgcgtgag ccggtggttt
     8341 tgatcaatgc cggcatgccg agaccagtca aagcttcatg caccagcgca gcggcttcga
     8401 gcatctgtga ccagctcgcg tctttcactg gatccaaatc gaaatgcaga taatcgggac
     8461 gatcgacatc atcgcagcgg ccgtaccacg gattgagatc gatgcagcca aggttgatga
     8521 cccagaccaa cgcagccaga tcagcgataa tgggaaaatc aatgatgttg cctgacgcat
     8581 gctcgattgc gcaggtttcg atccactcgg ggcgcggcga cggcgcccgc ttcatgaaaa
     8641 agaaatcgcc gccaatacca ttcggataac gtttcatgac cattgcgcga tcggccagat
     8701 gcggtagaag aaccggggcg atgtcgatgt aatactcgag aagatcgcgt ttggtgatgc
     8761 cgagatccgg ccaaaaaatc tttcgcagat tcgttaaatg gacgagtttg ccattggttt
     8821 cgaacgcgtc agtgtcttgg ccaccgcgca cgcgcaccga tcggttctga gatgagcgac
     8881 tctttaccgg ccgtcggacg gcttttccag gtggctgccg catacagagg atctccacgg
     8941 cgggcgattt tgcattgaaa acgccctctc ctcgctatca atccagcgta acggctttgc
     9001 ggcttgtttc aaggtggcaa tcggtcggag gtctacttgc cgatgtagag tttacgcgcg
     9061 gcgcgtaggt gggtgaaagc ttctgtcttg tttcccaggt tttcgagcag caaaccgaga
     9121 ttataatggg catcggcgaa atccggatca cggctgaggg attccttata acattcgacc
     9181 gcttcgcgcg gacgcttcaa gtcttcgagc aagacaccca gattgaagta cgccgctggc
     9241 tcacgcgacg cgagtcgtgc ggcggcgcgg tagtgcgtct cggcttggtc caattgaccg
     9301 tcctcgtggc agagctttcc caaattgagg tgagcgtcgg cgatattcgg gtcgatctcc
     9361 aacgcccgac ggtaagctcc ctgtgcttct tcggcggaag tcgcttcgag ttcagtaccg
     9421 agcttgaacc agtaatcggc gccaagatct tttttcgcgc gttttgatgg tggaagcttt
     9481 attgcagcga taacgctgcg cgcatcgaaa ttgaacagga actgtcccga atcgggttgc
     9541 cagcgcgcgt ggccgtccca gaccaccact ctgcggccat cggcatatat cttgacactc
     9601 gacagatgtt gctcgtcggg caattgccgc ttgagagatg aaaggatgcg cacgattctt
     9661 tttgccggaa cgcgtgacgc gagcaggctt ttagcggttt ttaaaaacag cagatcttga
     9721 aacgtaaatt gaagcgactt ggtatcgtcg cgccccggcg cgagaaatcc ggcgcggacg
     9781 aaggtacgga tccggctatc cggcaagttc aggattttcg ccacctccga cgtggagaaa
     9841 agcttcattt ttttgacgac gctttttttc tgggtttttc aggttcggct tgctttgcac
     9901 tgaccgccgg ccgcttctgc cgcggtccgg ttttctccag actctcttta agcgccgcca
     9961 tcaaatcgat cacctggcca cgctgcgcga caggttgggc cagagtaatt tccttgccct
    10021 cggctttctc gttgacaagt ttcaagactc ggtccgagta ttcatcgtgg tatttttccg
    10081 gctcgaattc gtcgttcgac aactcgtcga tgagacgaat ggcgaggctg gtttccgcgc
    10141 ccgtgattct ggccgactcg cctttgggaa tttcgtcgaa gttgcgcact tcgttagcga
    10201 agtacatagt gtgcatcatc aggccctttt gggccgcccg aacgatcacc aaattttcct
    10261 tgccgcgcat gacaaatttc gccagggcga ccttgccgac ctgcgtcatg gtatcggtaa
    10321 gcagacggta cgctttttca ccgcctttat cgggtccgag ataatagctg cgctcgaaat
    10381 aaatggggtc taccttcgag agcggtacga actccgaaat ttcgatggcc tcggaagcct
    10441 caccttcgag cgctttcagc tcatcatcgg tgacccgaac gtattggtcc ttggcaaatt
    10501 cataaccttt aaccaactgg tcccgctcaa cgacttcatt gtcaaccggg cagacgcgtt
    10561 gctgacgaat gcgattgccg catttcgcgt gcagcagatg aaaactcggc gcttgcgatg
    10621 aagtcgcaac gtagagctta accggtatcg acactaagcc aaaggaaata gcgcctgaac
    10681 caatcgagcg tgggggcatg aggacctcct tctgcaattt tctatcgtat caaatccggc
    10741 tgtttcaagt ctagcacgca aaccggaacg ggttgaccgc gtacgattga cagttcgaac
    10801 agatctagag taggacctga gtagaaatat ttcagcgcca atcaggagga ataacgaatg
    10861 gcaacctatc agactttgct ggtggataag caagacggca tcacgaccat taccttgaat
    10921 cgccccgaga aacgcaatgc catgagcccg caactgcatc gcgaaatgta tgactgcttg
    10981 accgatctca aatacgacca ggaaaccagg gtcatcgtga tcaccggagc cggcgaaagt
    11041 ttttgcgccg gtcaggatct caagcagtac ttcatcgaga tggattcgca gcccgatcgt
    11101 gtacgcgacg aggtgcggga aaaatcgcgt cagtggcgga acgaaatgtt gcgcaagatg
    11161 ccgcagccgg tcatagcgaa aatcaacggc tgggtgtttg gcggcggctt taccgtggtc
    11221 acgggttccg acattgcgat tgcggccgac gacgtgcaat tcggtctctc tgaagtcaat
    11281 ttcggccatt ttcccggcgg tgaagtcacc atcgtcctga cggagcatct gcagccgaag
    11341 cacgggctgt attatgcgtt gacaggaaaa accatgaacg cgaaagaagc ggagcgcatc
    11401 ggcttgatca ccaaggcggt gccgcgcgcc gatttggatc gggaggtatc ggagctggcc
    11461 aacaatctca aggaaaaagc cccgtctgcg ctgaacgccg tcaaagaagc gtggtactac
    11521 accttctact cgaatcccga cgtcgcgtac gaaatatcgg gcttgatttc ggataaagcg
    11581 aaagcggcca ttggcgggcg acccggtcta cagcagttcg cgcgcaaaga gtaccggcca
    11641 gggttgggat cgtataagtt cgaagaaaaa aaataaccgt tcgaaacgtt caagccgttc
    11701 aagaagtaac ggagcgaagc gatggaatgt tagtagtcgc ttcggaggat atgcatggat
    11761 tttgatctgc cggaagagct gcagatgctc aaagatacgg tgcggaagtt cgtagataga
    11821 gaactgattc cgatcgagat gcacacgatc gagaatatgg agctgaagcc agatattcgc
    11881 gagcgcttgg aaaagaaaac caaagaaatg gggctctgga tgttcgacgt acccgaagag
    11941 tacggcggtg gcggactcgg cagcctcgct caggtcttgg tatgggagga attggcgcgc
    12001 acggtcgcat tgccgtcgcg cggacaggga atcttcgggc cggaaatccg gccgatactc
    12061 tatgcgctga acgcggagca gaaaaaacgt tttctggatc cgatcagaaa cggggaaaag
    12121 aagctctgct tcatgcagac cgagcccgac gccggttcgg acccggcatc gatgaaaacg
    12181 cgcgctgtgc ggcagggcga ttattacatc gtcaatggca ccaaacgctt catcaccggc
    12241 gccggcgacg cggatttcgg ccagctaatg gcagtgaccg atccggcgaa gggcgcgcac
    12301 ggcgggattt cctgcttcat ggtcgacatg aagacaccgg ggctgacgct cgtgacgcgc
    12361 tacaaaacaa tcatgggcga ggagccctgg cacattactt tcgacgatat gaaagttccc
    12421 gcggaaaacc tcgtcggcaa agaaggggaa ggattcaagc tcgcgcaaaa atggctgggc
    12481 atcggcaggt tgaagcacgg cgcgcgtgct ttgggagttg cggagcgctg catcgagatg
    12541 ggcgcgcgct actccaagga gcgcgtcacg ttcgggaagc cgctgtctga acggcagggg
    12601 atcaccttca aactggcgga ttcttatgtc gagctgcacg cggcgcgact gatggtctat
    12661 caagccgctt ggaaaaacga tcagaagcag gacattcgaa atgaagcgta tatggcaaag
    12721 ctattcgccg atgaaatgtc gtttcgtgtg gtcgatcgcg tattacaaat ccacggcggg
    12781 atcggcctga cattggactt gcctttggcc aagtggttcg tcgaccagcg cagccggatg
    12841 atcaccgagg gcgcgtccga ggtcatgcgc atggtgatcg cgagggaagt gctgagaagg
    12901 tacaattaag agttcaaaac gttcaagccg ttcaagatgt tcaaccggta gggattaatt
    12961 cagacagttc caacgacttg aaccatttga acggttttaa ctgccttatt ttgtaaacga
    13021 caactcccac cggttgccgt cgggatcgaa gacgttgatg gaataattct cgccatcgcg
    13081 gtgcggacga accttttcaa tattggcacc gatgttttgc agcgcggcaa gtgcctgctc
    13141 tttttcctgc caaggaatgt tcaatgtgtg atgcggatcg acttcgcgcg gcttgatcga
    13201 cgcatcgaac aggtgcactt cgaaattgcc cagacgtacg tccacttctt tcacctgtcc
    13261 agcttttttc tgctcttcag tcggctcgac ggatttgacg atttgaccgc ctaaagtctt
    13321 agtgtagaac tccaccgact tgtcgaggtt ctttgcgctc atggcgaacg ttcgaagtcc
    13381 cttgatatcg aacatgctaa acctcccgta aagaatagcg taattcgctt accgcttaac
    13441 attccgttga atcggctgtc aatctggctg attcacggat gattgcaagg cacaaggcta
    13501 aggcagggct tgcaccaagc gcgcactgcc gatttctcaa tcgcagacaa gtctttgggt
    13561 tctactgctc catcaaaatc tttacccgcg caacaacttc ttccggctgg ttcatgacga
    13621 gctttgctag gctttcaagc tcttcgcccg tcgaaggcgt aatatcgaag tttttgcgct
    13681 tcgcttcggc cagcagttcg gggtcgttca tagtcttcag aaatgcttcg cggagaattt
    13741 tcagccgatc cgctggtatt ccgggcggcg cgacgatcgg ccggccgaaa tcggttgccg
    13801 ccataaccag cggcaccaat cgtctctccc tctccggtgt tttaaattgg tccatcaatt
    13861 cgccgagcgt cggtacatcg gggagccgcg cgtcgcgcgt ttgcccggtc tgcatcaaaa
    13921 ttcgtgcgaa accgttttta cgccaggtga agtagggctc ccgcgagtga taggcttgaa
    13981 cagtaaatgc gcggcagtgg agctcgccac gttcgacggc gagatcaatt tcgccgccgc
    14041 cctgataacc ggcaacaata gtaaatttcg ttcccagggt ttcttcgagt agcttcggca
    14101 tataactgcc ggaggttccc gctcctgtgg aaccgcattt gggcggctcc ttggctctac
    14161 gcacatcggc caaagttttg tacggcagat cggcgcgcat gtagagaagg taatcggatt
    14221 tgtccgagct gccgatccag ccgaattttg cccagtcgta cttcacttct ttctgcttca
    14281 tgagctggtt gaagtagatg gacggctgaa tcgccccaat ggttaatcca tcgggcttag
    14341 aaacgctgta gacgtaatta gcgccgatga tcgatccagc gccggtcatg ttttgaacaa
    14401 tgaagctggg atttcccgca atgtgcttgc ccatgaactg cgccatcaaa cgtgcgtaga
    14461 gatcataagc gctgcccgcg ctgacccccg cgacgacggt gatggtcttg ccctgataga
    14521 acggagtctg cgcccatgca gccggagtga agaaaagcca gatgagaacg aaccaatgtc
    14581 gttgcataat ttattgtgag gcttggaaag atttacagtg aatagaaccg cttcggatta
    14641 tcagtcagaa ttttgttggc caaatcagga ggcaaatcct cgcgagatct tatggtctgc
    14701 accaatgcct tctcctcggc cgggtcattg tggccgtagt ccgagccgat catcaaatga
    14761 tctgcgccga tgaattccgc gagataagga atatcttcgt cg
//
LOCUS       DASULZ010000263         3590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_540199, whole genome shotgun sequence.
ACCESSION   DASULZ010000263 DASULZ010000000
VERSION     DASULZ010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540199"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(332..3565)
                     /locus_tag="VFY96_05545"
     CDS             complement(332..3565)
                     /locus_tag="VFY96_05545"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF020044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5605958.1"
                     /translation="MGYLTQQIADEVGRLTQERGRQYFLRGAVESIEGDNFSINAVVQ
                     GTMRYRVQIKNLNEFIDYSCNCPFFERDFEPCKHIWAAALKAEQHGYLRGRDKLSSVA
                     FNSEHKTFGPARSGASGSGLSWQKQLEPLFHSMALVESRSAVTAPAEREFLYIVDGTA
                     TASADRLIIEVAQRELRSNGTWGKLKTHKLKLNDLDLIPAAEDRRIIAMLAGSLRDSI
                     DDYSPYYQQSIFPQFALQPSLWEIVLPLICATGRCLLRVSDATEELLPLHWDDADPWD
                     FYLRLAPAENDAAYRLSGGLRRGDFEMSLSKPLLILAGGLVFFDGQVARLNDHRAFPW
                     ITLLRQRGELSIPKQDSEPFLAEILRFAHYPRLELPEELRFQEALLRPKPKLLIKPGP
                     SHPFRHPRLVGKLAFDYDGHKVFHNTPGNVYDQERRRLIRRDFGFESAARRRMIQAGF
                     RTINSNGDANAELYADQLPKVVRTLTQEGWDIEAEGKLYRTAEKLKIEVKSGVDWFEL
                     DGSATFGDAKISLPQLLRAIQQGESMVKLDDGSLGVLPEDWMKKYGLLAGLGNLQDKH
                     LRFTRPQAGLLDALLAGEPAVSVDSAFARLRQELRDFSGITAADPPMGFTGKLRAYQS
                     EGLAWLFFLQRFGFGGCLADDMGLGKTIQALALLEARREVRLGRKENPPSLVVVPRSL
                     VFHWKKEASAFAPKLRILDHTGATRLKPGDHFADYDVVLTTYGTLRRDALQFKDQRFD
                     YCILDEAQAIKNSGTLSAKAVRLLRADHRLAMSGTPVENHLGELWSLFEFLNPRMLGS
                     ASIMVNAGRSSDEETRAFLGKALRPFILRRTKSQVATELPEKTEQTLYCDLQSDDRKL
                     YDELRDYYRSRLLKRDPSGAAGQFKLQVLEALLRLRQVACHPGLVDKRRIEKGSAKLD
                     TLLAQLDQVLEENHKVLVFSQFTSFLALVRARLDAAEISYAYLDGRTRDREARVEQFQ
                     NDPNTKLFLISLKAGGVGLNLHAAEYVYLLDPWWNPAVEAQAIDRAHRIGQTRQVFAY
                     RLIARDTVEEKVLELQKAKRDLADAIISADNSVIRNLTREDLELLLS"
BASE COUNT          813 a         1027 c          973 g          777 t
ORIGIN      
        1 gctcaaggac gagcccattt tgatttttgc gctctgcgca tctcccatcg aaggccaaag
       61 caacagtccg aaaccaagtg cggtccagcg ccacttccaa ttcatttttc tatctcctgt
      121 agtagttttt cgccaaattt cgctgcccag tcattcagga aatggagaat gccacccata
      181 gcacgaccct cggataacgt ccacacgggg cggctcagcg atgagtcggc agtttaaacg
      241 tgcaacattt cccgttccac tggaacggac accaagagag atctttggtt aactgaggca
      301 gcgccccggc catcggttcc cagcttgacg ttcacgacaa cagcaattcc aaatcctctc
      361 gcgtcagatt tcggatcacg ctgttgtcgg cgctgatgat cgcgtccgcc aaatcgcgct
      421 tggccttttg cagctccagc actttctcct ccacggtgtc gcgcgcgatc aagcgatagg
      481 caaacacctg acgggtttgc ccaatgcggt gcgcgcgatc gatcgcctgc gcttccacgg
      541 caggattcca ccaggggtcg agcaggtaaa catattccgc cgcgtgcaag ttgagtccga
      601 cgccacctgc cttgagactg atcagaaaca gtttcgtgtt cggatcgttc tggaactgct
      661 cgacccgtgc ttcccgatcg cgggtccgac cgtcgagata ggcgtaagaa atttccgctg
      721 cgtcgaggcg ggcgcgtaca agcgcgagga aactggtgaa ttgggaaaat accaaaacct
      781 tatgattttc ttccagcacc tgatcgagtt gcgctagcaa agtatcgagc ttagcacttc
      841 ccttttcgat ccttcgttta tcgaccagtc cggggtggca cgccacctga cgcaagcgca
      901 acagcgcttc cagaacttgc agcttgaatt gaccggcagc acctgacgga tctcgcttta
      961 ataagcgcga gcggtaatag tcgcggagct cgtcataaag cttgcgatca tccgattgga
     1021 gatcgcagta cagggtctgc tcggtttttt ccggcagttc tgttgccacc tggctcttgg
     1081 tgcggcgcaa aatgaaaggc cggagagcct ttcctagaaa cgcccgcgtc tcttcgtccg
     1141 agctacgccc ggcattgacc atgatcgaag cgctgccgag catgcgcgga ttgaggaact
     1201 cgaaaaggct ccaaagttcg cccaggtgat tttccaccgg tgtgccgctc atcgccaggc
     1261 gatgatcagc tcggaggagg cgcacggctt tggcggacag tgtgcccgaa tttttgatcg
     1321 cctgtgcttc gtcgaggata cagtaatcaa accgctgatc tttgaactgc agtgcgtcgc
     1381 gccgcaaagt tccgtaggtg gtcagcacga catcgtaatc cgcgaagtga tcgccgggct
     1441 taaggcgcgt tgctccggtg tgatcgagaa tgcggagctt gggggcaaac gcggaagcct
     1501 cctttttcca atgaaatacc agcgagcgcg gtaccaccac gagcgatggc gggttttctt
     1561 ttctgcccag gcgcacctcg cggcgcgctt cgagcagcgc cagcgcttga atcgttttgc
     1621 ccagtcccat atcgtcggcg agacagccgc caaaaccaaa ccgctggaga aaaaacagcc
     1681 aagctaatcc ctcggactgg taggcgcgga gcttgccagt gaaacccatc ggcggatcgg
     1741 ccgcagtgat ccctgaaaaa tcccggagct cctgacgcaa acgcgcgaag gcggaatcca
     1801 ccgaaaccgc aggctcgccc gcgagcagcg catccagcaa gcccgcttgg ggccgcgtaa
     1861 accgtaggtg cttgtcctgc agattgccaa ggccggcaag tagaccgtat tttttcatcc
     1921 agtcctcggg caacactccg agggagccat catcaagctt gaccatcgat tcgccttgtt
     1981 gaatggcgcg cagcaactgc ggcaaagata ttttggcatc tccgaacgtt gcgcttccgt
     2041 ccagctcgaa ccaatcaacg cctgatttga cttcgatctt tagcttttcc gccgtacggt
     2101 atagttttcc ctcggcctct atgtcccaac cttcttgcgt cagagtccgt acgactttgg
     2161 gtaattgatc cgcgtacagc tccgcattcg catcgccatt actattgatc gtgcgaaatc
     2221 cggcttggat catgcgccgg cgcgcagcag attcgaaacc aaaatcccgg cgaattagcc
     2281 gacggcgctc ctgatcataa acgttgccgg gggtattgtg gaaaaccttg tgcccgtcgt
     2341 aatcgaaagc caacttgccg accagccgag gatggcgaaa gggatggctc ggtcctggtt
     2401 tgatgaggag tttcggctta ggccgcagta gagcttcctg aaaccggagt tcttcgggca
     2461 gttcgaggcg agggtaatgc gcaaaccgca aaatttccgc cagaaacggt tcactatcct
     2521 gcttgggaat cgacaactcg cctctttgac gcaagagagt gatccacgga aaggcccggt
     2581 gatcattaag tcgagctact tggccatcga aaaaaaccag accgccagcc aaaatcagca
     2641 aaggcttaga tagcgacatt tcaaaatcgc cgcggcgtag accgccggac agtcggtatg
     2701 ccgcgtcgtt ctcagccggt gccagcctca aatagaaatc ccatggatcg gcatcgtccc
     2761 agtgaagagg caaaagctcc tcggtcgcgt ccgaaacacg caacaggcaa cgtccggtcg
     2821 cgcagataag cggcagaacg atttcccaga gcgacggctg cagtgcaaac tgaggaaaaa
     2881 tggactgctg atagtacggt gagtaatcat caatcgaatc gcgcaaagag ccggcaagca
     2941 tcgcgatgat gcgccgatcc tccgcagcgg gaatcaaatc gagatcattg agcttgagct
     3001 tgtgggtttt aagcttgccc catgtgccgt tgctgcgcag ctcgcgctgc gccacttcaa
     3061 tgatgaggcg atctgcgctt gccgtggccg taccgtcaac aatgtagaga aactcacgtt
     3121 ccgccggggc ggtcacggct gatcggctct ccactagcgc catagaatga aacagcggct
     3181 ctagttgttt ttgccaggac agccccgatc cggaagcgcc ggacctcgcc ggaccaaacg
     3241 tcttgtgttc tgaattgaaa gctacggaag atagcttatc gcgacctctt aggtaaccgt
     3301 gctgctcggc tttgagagcc gctgcccaga tatgtttgca gggttcgaag tcgcgctcaa
     3361 aaaatggaca attgcacgag tagtcgataa attcgttcag gtttttaatc tggacacggt
     3421 aacgcatcgt gccttgcacg actgcattaa tggagaaatt gtcgccctcg atgctttcga
     3481 ccgcgcctcg gagaaagtat tgccggcctc gctcctgtgt aagcctgccg acttcatcgg
     3541 caatctgttg cgtcagataa cccatttata agacgcgcga tacagtgaaa
//
LOCUS       DASULZ010000264         3661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1046436, whole genome shotgun sequence.
ACCESSION   DASULZ010000264 DASULZ010000000
VERSION     DASULZ010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046436"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VFY96_05550"
     CDS             <1..651
                     /locus_tag="VFY96_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX5605959.1"
                     /translation="IVQYGLLHYIVNSAVVATATAVISIFVASLAAFALARFRLYWGR
                     YYLMLLLALSMFPQIAIAGPVWRLLSFLGWLNSYHGIVAAYIALSLPFAVWVLTTFFR
                     ELPYDIEEAALIDGCSRWQALRLIVWPLAAPGVFTAAILVFIQAWNEFFFALIILTYP
                     ALQTLPVGIALFPGEYTMPWGEIAAASTVATLPLVALTLLFQRRIVQGLSAGAVKG"
     gene            683..1747
                     /locus_tag="VFY96_05555"
     CDS             683..1747
                     /locus_tag="VFY96_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006698542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5605960.1"
                     /translation="MTVRQISKKFGAAAVLEEISFEVADGEFCVLLGPSGCGKTTLLR
                     IIAGLESATAGSVQIDEQRVDLLPPRERDVAFVFQNYALYPHMTVSENLGFSLRLRGA
                     SKEEINARIVEVARLLELDNQLNRKPQQLSGGQRQRVALGRAIVRRPKLFLFDEPLSN
                     LDATLRSNMRVELARLHERLRSTMVYVTHDQAEAMTLGEKVVVLDRGRIQQIGTPAEI
                     YHRPRNLFIAGFVGQPQMNFVQGRVTEGSLLVAKSGMNLDLSAILAEHWIGVSPTDVS
                     IGIRPEDLRPCSRTEAWFSGIADFVEDLGSDCFVHVIANGEKLIARAPGRTPIRRGDE
                     VFLCSDPERVHVFQGEKRFE"
     gene            complement(1897..2379)
                     /locus_tag="VFY96_05560"
     CDS             complement(1897..2379)
                     /locus_tag="VFY96_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5605961.1"
                     /translation="MPTIKGLSHVVLHVNDLEKMVSFYRDVLGLVKYREYPGRMVFLT
                     PDPKIEDHQLALTKGREGNAKIIAHIAWRVETPADVKEYYERFKALGVTIDHCVSHAY
                     EEMGNTVSCYFLDPEGNRLEIYALIPERDEKRINRPLDLDQTLDKIVAQASGLPANAL
                     "
     gene            2616..2921
                     /locus_tag="VFY96_05565"
     CDS             2616..2921
                     /locus_tag="VFY96_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605962.1"
                     /translation="MKKREIGLIEGFLLCLCDSHTVRLQTSQWKRALLVFSSGVCLEL
                     AALPLLAGLIGWEFPWFAWLLTAFFGPLGLLGLYASKFGDDRLVESLLVLPKLDLGL"
BASE COUNT          734 a          978 c         1026 g          923 t
ORIGIN      
        1 atcgtccagt acggtctgct tcattacatc gtgaatagcg cggttgtggc gacggcaacc
       61 gcggtgatca gtatttttgt cgcgtccctg gcggcgtttg ctctcgcacg gttcagacta
      121 tactgggggc ggtactactt gatgttattg ctggcgttgt caatgtttcc acaaattgcg
      181 atcgccggcc cggtctggcg cctgctgagc tttctcggct ggcttaatag ttatcacggc
      241 atcgtcgccg cctatatcgc gctttcgctg ccatttgcgg tttgggtgct cacgacattt
      301 ttccgggaac tgccgtacga tattgaagaa gcagctctca tcgatggctg cagccgctgg
      361 caagcgctgc gcctgatcgt gtggccgcta gcggccccgg gagtttttac ggcggcgatt
      421 ctggttttca ttcaagcttg gaacgaattt ttctttgcct tgatcatttt gacctatccg
      481 gcgttgcaga cgctgccggt gggtattgcg ctatttccgg gcgagtacac gatgccttgg
      541 ggggaaattg ccgctgcttc cacggtggcg acattgccgc ttgtagcttt gacgctactg
      601 ttccagcggc gcatcgtcca gggactttcg gcgggggccg taaagggctg agaaaaagct
      661 tacggaggaa ccgatcggct ctctgactgt ccgccaaata tcgaagaaat tcggcgctgc
      721 cgccgttctc gaagaaattt ccttcgaggt ggccgacggt gaattctgcg ttctgctcgg
      781 tccgtcaggc tgcggcaaga caacactgct gcgaattatc gccgggctcg agtccgccac
      841 cgcaggcagc gtccagatcg atgagcagcg cgtggatctc ttgccacctc gggagcgcga
      901 tgtcgcattt gtgtttcaaa actacgcgct ttacccgcat atgaccgtat cggaaaatct
      961 cgggttctct ttgcgcctgc gcggcgcatc taaagaagaa ataaatgcgc ggatcgttga
     1021 ggtggcccgg ctgttagagc tcgataacca gctgaaccgc aaaccgcagc aattgagcgg
     1081 ggggcagaga caacgggtgg cgctcgggcg cgccattgtg cgtcggccca agctttttct
     1141 tttcgacgag ccgctttcga atctcgacgc aaccttgcgg tccaacatgc gtgtcgagct
     1201 cgcccggctg catgagcgcc tgcgctcgac catggtttac gtgacgcatg accaggccga
     1261 ggccatgact cttggtgaaa aagtcgtcgt cctcgatcgc ggtcggatcc aacagatcgg
     1321 cacgcctgca gagatctacc atcgtccgag gaatttattt atcgccggct tcgtcggtca
     1381 gccgcaaatg aatttcgtcc agggtcgagt gactgaaggt agccttctcg tggccaaatc
     1441 gggaatgaat ctcgatctga gtgcgattct ggccgaacac tggataggag tttcgcccac
     1501 cgacgtctcc atcggtatac gcccggaaga tctccgcccc tgctcgcgca ccgaagcctg
     1561 gtttagcggc atcgctgatt ttgtcgagga ccttggttca gactgctttg ttcacgtgat
     1621 tgccaacgga gaaaagctga ttgcccgtgc gccggggcga acgccgatca ggcggggcga
     1681 cgaggttttt ttatgcagcg atccggagcg ggtgcatgta ttccaaggcg agaaacggtt
     1741 cgaataattg ctagtcgtga acggcaatga tgcgagcggg acgtcaatac tgttatttcg
     1801 agcgcgagcg aattttcttt ccgtgttgcg cgtctttgct aacaacgcga ggctaagatt
     1861 taacacgctc gcttaaaacc attaacgccg gaatctttac aacgcgtttg caggcaagcc
     1921 gctggcttga gcgacgatct tgtcgagggt ctgatcgagg tcgagcggtc gattgatgcg
     1981 tttttcgtcg cgctcgggaa tcagcgcgta gatttccagg cggttccctt cgggatcgag
     2041 aaaatagcac gacaccgtat tgcccatctc ttcgtaagca tggctgacac agtggtcgat
     2101 ggtgacgccg agcgctttaa agcgttcgta gtactccttg acgtctgcag gtgtttccac
     2161 acgccaggcg atatgggcaa tgattttggc attgccctcg cgccctttcg ttagggcgag
     2221 ttgatgatct tcgattttgg ggtcaggggt gagaaagacc atacgacccg ggtactcgcg
     2281 atatttgacg agaccgagaa cgtcccgata gaaggagacc attttttcca gatcgttcac
     2341 gtgaagcacc acatggctga gtcctttgat ggtcggcata gttcctcctg gctgggttag
     2401 gcgagacagc tgaattcgag ccgtttaaac gcctcatccg gaaagttatc cgatcaattt
     2461 agcatctgtc aatcgaaact gcttggagca atttgctttc tgacaaaagg ggccttaaca
     2521 tatgcggacc aaaaaaacga aactttgccg cgttcattag agcatcgcat aagccgtcgc
     2581 tccgtcagca gcccaagtcc gggaggaaac aagagttgaa gaaacgagag ataggattga
     2641 ttgaaggatt ccttctatgt ctgtgtgact cgcacaccgt ccggctgcag acgagccaat
     2701 ggaaaagagc acttctggtt tttagcagcg gtgtttgcct cgagctcgct gcgttgcctc
     2761 ttttggctgg gctgattggc tgggaattcc catggtttgc ctggttgtta acggcatttt
     2821 ttggacccct cgggctactc ggcttatacg cgagcaagtt cggcgatgat cgtctggtcg
     2881 agtcgctgct ggtcctgcca aagctggatc tcggcctcta aggtcatcgc aaaatgagca
     2941 atcttgattc gcagttttac gcctgctagc tgtgattagc ggggtcacgg tgagggtcga
     3001 taggaaaatt ttgtctctac gtcgctggtc tggtttcttt atcgcggcgc ttttccttac
     3061 gttcctgact tattcaaccc cccatcaagt ccatcacgtt ttcgatcacc ttcggcattc
     3121 gcaacaacat tcggatcatc acactagcac cgatcagccg cccgccggca aacagaactc
     3181 agcctgtgtc ttcgagtcgg ttgcgagccg ttgccatgca aatgccgctt catccgttca
     3241 gctgtcgggg attaatgctc cagtacagtt cttcacgggg atttcgaaaa cggccggcca
     3301 tcatcgattc attccagcaa ttttccaaat ccgcgctcct cctccttctc acctgatcgc
     3361 acaattctaa agtaagctag tttcgctgac attcggcgtc aggcctgccg agtggcgaaa
     3421 ccactgtttc catcaattac ccggcgctgc gtagcgcgtc ggagaaagct ggatcatagg
     3481 tgctcgatcc ggagagatcg tcatgaaacc acggcgttcg accgtttggc ttgctgcatt
     3541 cagtaagctt tggatgttta gcattgcatt tggctgcgtg atcccggcgc aagctgccgg
     3601 ttccggctcg ttctcgttca acactcaggt gcagttgggc cggcgcagca acaacccatc
     3661 c
//
LOCUS       DASULZ010000265         2960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1149242, whole genome shotgun sequence.
ACCESSION   DASULZ010000265 DASULZ010000000
VERSION     DASULZ010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149242"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..1085)
                     /locus_tag="VFY96_05570"
     CDS             complement(21..1085)
                     /locus_tag="VFY96_05570"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605963.1"
                     /translation="MSNFSVRQKTMLVLAGFVFYAVQAIGAPALANPYLAKPGALPLK
                     IRIGTCAVTGGFVHLYTALDHRLFDKYGIQAEHVVVRGGNVAMAALAADEISFLYCNA
                     DANIARIGAGADGKLVAAVLIGLPYVVLACKDIKKPADLKGKSIGVTRPGDFAYRLAK
                     EFLKKFNLSDKEVTLATVGGTPTERYAALAQDVFQATLIQPPLDARGRKDGFNVIYRL
                     KDLGFPFIYSSLFTNSRSLRERPEIVQKAVAGLAEAIYFVEKNPQLAMNSVGKTLRIT
                     DADTLRSAYDTYARDLINRRMLVPAKMVSDTLELAQQDGAIIRRKPNDIFDNSFVENL
                     EKSGFLKELWGGALPEERKR"
     gene            1212..>2960
                     /locus_tag="VFY96_05575"
     CDS             1212..>2960
                     /locus_tag="VFY96_05575"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEX5605964.1"
                     /translation="MENNREFFNLYNHGFIRVAVGVPEARVADPVFNTARTIELMEQA
                     EHDRAVLTLFPELGLSAYSCDDLFHQRALLDASLASLQRILEVSKQLGLIAIVGMPLQ
                     VDHLLFNCGVVLHNGKILGIAPKTYLPNYREFYEARQFAPACAAHSSAVELLGQSGIP
                     FGEEILFKSAKNPGFTFFVEICEDLWVPIPPSSKAALAGATVILNLSASNITIGKTEY
                     RHNLVANQSARCLAAYVYTAAGTGESTTDLAWDGHAIIYENGDLLAESERFRYDSHWI
                     TADIDLERLTQERMRQNSFGQSMERYAEELRSFRTVSVDIELPTNGQLLANRQIERFP
                     YVPADPELRNRRCYEAYNIQVQGLVKRLQSSKLSKIIIGISGGLDSTQALIVAARAMD
                     VLNVPRTNILAYTMPGFGTSQRTAANARALMEAIGCSANELDIRPSCEQMLKDIDHPF
                     AHGQPVYDVTFENVQAGERTSHLFRLANLHNGLVLGTSDLSELALGWCTYGVGDHMSH
                     YSINASVPKTLIQFLIRWVADTNQLGGSTSGVLRSILATDFSPELIPGDPAREQPTQR
                     TEDVVGPYELQDFTLYY"
BASE COUNT          659 a          829 c          786 g          686 t
ORIGIN      
        1 acggcgcgcg acccaatttc ctaccgtttt ctttcctccg gcagcgcgcc gccccacagt
       61 tctttgagga atccgctttt ttccagattt tcgacaaagc tattgtcgaa gatatcattc
      121 ggcttgcgcc gaatgatcgc cccgtcttgc tgggcaagct cgagggtatc gctgaccatt
      181 ttagccggca ccagcatacg ccgattaata agatctctgg cataggtatc gtaggccgaa
      241 cgcagcgtat ccgcatcggt aattctcaaa gttttgccga ctgagttcat agcaagctgc
      301 ggattttttt ctacgaaata aatcgcttct gccagcccgg ccaccgcttt ttgcacgatt
      361 tccggccgct ctctcaagct cctggagttg gtgaacaggg agctgtaaat gaagggaaac
      421 ccaagatctt tgagtctgta aatgacattg aagccgtcct tccggccacg cgcatcgagg
      481 ggcggttgaa tcaaggtcgc ttggaagaca tcctgcgcca gcgctgcata gcgctccgtc
      541 ggcgtgccac ccaccgtcgc caatgtcact tctttgtcgc tcagattaaa ctttttgaga
      601 aactctttgg cgagacgata cgcgaaatct ccggggcgag tgacgccaat gcttttaccc
      661 ttcagatctg ccggtttctt gatgtctttg cacgcgagga ccacataggg aaggccgatc
      721 aacaccgccg ctaccagctt gccatcggcc cctgccccga tccgggcgat attggcgtcg
      781 gcgttacagt agagaaaact gatttcatcg gcagcgagcg ctgccatcgc gacattgccg
      841 ccgcgcacca ccacatgctc ggcctggatt ccatacttat cgaagagccg atgatccaga
      901 gcagtgtaga gatggacaaa accgcctgtg accgcgcacg taccgatgcg gatttttagc
      961 ggcagagcgc cgggcttagc caaataggga ttcgctagcg caggagcacc gatcgcctgg
     1021 acagcgtaaa aaacaaaccc cgctagaacg agcatcgttt tttgccgaac ggaaaaattg
     1081 ctcataggga tggctcccga agtcaagcag aatggattat gtagatattc cctgcatcgt
     1141 ctccgtcaat gtcccgccag cgccattttc cctggctcgc aaattagcct atagtacgtc
     1201 acaggtgaga catggaaaac aaccgggagt tttttaacct ttacaatcat ggatttattc
     1261 gggtcgctgt gggagtgcct gaagcacgcg tcgccgatcc tgttttcaat acggctcgta
     1321 ctatagagct catggaacaa gctgagcacg atcgggctgt cctcacgctt tttcccgaat
     1381 tggggctttc cgcctactcg tgcgatgatc tgtttcacca gcgagccttg ctcgatgcct
     1441 ccttggcttc gctgcagcgc atccttgaag tctcgaagca gctcggtctc atcgcgatag
     1501 tgggcatgcc gctgcaagtc gatcatctcc tcttcaattg cggcgtggta ctgcataacg
     1561 gaaaaatcct gggtatcgca cccaaaacct acttgcccaa ttaccgcgaa ttctacgagg
     1621 cgcggcagtt tgcgcccgca tgcgccgctc acagttcggc ggtagaacta ttggggcaaa
     1681 gcggtattcc cttcggtgaa gaaattctgt tcaaatcggc caagaatccc ggcttcacgt
     1741 tttttgttga gatctgtgaa gatctttggg tgccgattcc accttcgtca aaagccgcgc
     1801 tcgcaggcgc cacggtaatt ctcaatctct ccgcatcgaa cattaccatc ggtaagacag
     1861 agtatcgtca caatctggtt gccaatcagt ccgcgcgctg tctcgccgca tacgtgtaca
     1921 cggctgccgg cacgggcgaa tcgacaacgg atctcgcatg ggatggccat gcgataattt
     1981 acgagaatgg agatctgctc gccgaatcgg aaaggtttcg ctacgactcg cattggatta
     2041 ccgccgatat cgatcttgaa cggctgactc aggagcgcat gcggcaaaac agcttcggtc
     2101 aaagcatgga gcgttatgcc gaggagttgc gcagtttccg cacggtgagc gtcgatatcg
     2161 agctgccgac caacgggcaa ttacttgcga accggcaaat cgagcgtttt ccttacgtcc
     2221 ctgcggaccc ggagctacgc aatcgtcggt gttacgaagc ctataacatt caagtgcaag
     2281 ggctcgtgaa aaggctgcag agttcgaaat tgtcaaaaat catcatcggt atttccggcg
     2341 ggctggattc cacacaggcg ctcatcgtcg ccgcgcgtgc catggatgtt ctcaacgtcc
     2401 cgcgcacgaa cattctggcg tacaccatgc caggattcgg gaccagtcag cgcacggcag
     2461 ctaatgccag agcgctgatg gaagcgatcg gctgctcggc caacgaactg gatatccggc
     2521 cgagctgtga acagatgctg aaagatatcg atcatccgtt cgctcatggt caaccggtct
     2581 acgacgtgac gtttgaaaac gttcaggcgg gagagcggac cagccatcta tttcggcttg
     2641 ccaatcttca caacgggctc gtcctcggga ccagtgattt gagcgagctt gcgctgggtt
     2701 ggtgcactta cggcgtcggc gatcacatgt ctcattacag catcaacgcc agcgttccca
     2761 aaacactgat tcagtttctg attcgttggg tggctgacac aaaccagctt ggcggtagca
     2821 ccagcggggt gctgcgctcg atactagcga cggatttcag tccggagctg attcccggtg
     2881 acccggcgag ggaacagccg acgcaaagga ccgaggatgt tgtcggtccg tacgagcttc
     2941 aggattttac gctttattac
//
LOCUS       DASULZ010000266         2921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1352459, whole genome shotgun sequence.
ACCESSION   DASULZ010000266 DASULZ010000000
VERSION     DASULZ010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352459"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..809)
                     /gene="rnc"
                     /locus_tag="VFY96_05580"
     CDS             complement(96..809)
                     /gene="rnc"
                     /locus_tag="VFY96_05580"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX5605965.1"
                     /translation="MTNETRLSRLQKALDYNFKDGALLLRCLTHVSYERRKGEAHNEV
                     LEFLGDAVLDLAVSDLLIRRNPEKSEGVLSKMRAALVNSTVLAEKATQLAFGPLLRIG
                     KGEELSGGRAKSSILAGAFEAILGGVYQDGGYEAARRVVERFFAAEVAGKTLGQHDYK
                     TRLQEISQMIFHALPSYRIVSETGPDHEKFFVTEIAVAGKVLGTGSGKSKKQSQQEAA
                     KKALDALQSDVHPYPKAAD"
     gene            complement(856..2160)
                     /gene="mtaB"
                     /locus_tag="VFY96_05585"
     CDS             complement(856..2160)
                     /gene="mtaB"
                     /locus_tag="VFY96_05585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEX5605966.1"
                     /translation="MRVAITTLGCKINQYDSAVIQSRLEHEHSMVPFEASAECYIINT
                     CTVTDRADWEARQLVRRAKRRNPQAKILVTGCYAQVNPHDVARVPGVDYVVGLNRLDD
                     LIRFIAADRPTAPQITVSDVKRERGVPVIGTRALPGHTRAFLKIQEGCNYSCTYCIIP
                     AARGLSRSVAPREVMEQVKQLAGAGYKEIVLTGIHLGGYGQDLRPKIDLTELVEMIAA
                     SGLISRLRLSSLDPRELPERLIDLIASSQMVCPHLHVCAQAGDDETLTRMRRNYNTGY
                     YRDLLARVRERLPEAALGSDIMVGFPGESEQQFESSLEYFASLPLTYFHVFPYSSRRG
                     TPAASLADQVPVHVKKTRARRLRDLGVQKKRAFCSSFLGRRLAVLIEDKMDVKTGARR
                     GFTRNYLPVNVRSSELSNEEVIVELTGFDNGWLVGAACSAQI"
     gene            complement(2249..>2921)
                     /locus_tag="VFY96_05590"
     CDS             complement(2249..>2921)
                     /locus_tag="VFY96_05590"
                     /inference="COORDINATES: protein motif:HMM:NF042466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyltransferase beta-barrel
                     domain-containing protein"
                     /protein_id="HEX5605967.1"
                     /translation="ADRAKDQSYFLFALRQEQLARVLFPLGEMQKTEVRERARRLRLP
                     AAERPESQDICFGDYKDLVASYAEKDELRGGDVINRSGKVLGRHHGIHSVTVGQRRGL
                     GIAAAEPLYVIDIDDTTKQVTVGKKAELRCTGLIASGVNWIDLPDQVEFAAEVQIRYR
                     APAVPCMVRSDANGSCEVVFHQTVHGVTPGQAAVFYRSDEVLGGGWIERAMKEERQET
                     AGSRP"
BASE COUNT          624 a          866 c          784 g          647 t
ORIGIN      
        1 ccgcagctcc agccccaacg attagcacgt ccgctgaaat tcgagccatg tggaatgaac
       61 gctccaggcc ccttcaatcg ggggctattt agatgtcaat cggcggcctt tggataaggg
      121 tgaacgtcac tttggagtgc atcaagagct ttctttgcag cttcctgctg agattgcttc
      181 ttgctcttgc cggaacctgt tccaagaact ttgccagcga cggcaatctc ggtcacgaaa
      241 aatttttcgt gatccgggcc ggtctcggag acgatacgat aggatggcag cgcatgaaag
      301 atcatttggc taatttcttg aagtctcgtt ttatagtcgt gctgtcccag ggtttttccg
      361 gcaacctcag cggcgaaaaa tcgttcaacc actcgacgtg ctgcttcgta gccgccgtcc
      421 tggtaaacgc cgccgaggat cgcctcgaaa gcgcccgcaa gtatggatga cttggcacgg
      481 ccgccactca attcctcgcc cttgccaatg cgcaggagcg gaccgaaagc gagctgagtc
      541 gccttttcgg caagaactgt agagttgacc agcgccgcgc gcattttgga tagcacgcct
      601 tcgctcttct cgggattacg gcgaatcaac aaatcgctca ctgccaaatc taaaacagcg
      661 tcgccgagga attcgagaac ctcgttgtgt gcttcgcctt ttctacgctc gtaggacaca
      721 tgggtcaggc agcgcagcag cagcgcgcca tctttgaaat tgtaatccag cgctttttgc
      781 aagcggctaa gtcgcgtctc gtttgtcact ttgattctat tctggagaaa ccgcgacgcc
      841 gtcgcaagga gccggttaga tttgagcgct acaggccgcc cccacaagcc agccattgtc
      901 aaatccggtc agctcgacga tgacctcttc gttggacagc tcgctgctgc gaacgttgac
      961 cgggagataa ttccgggtga agccgcgccg agcgccggtc ttcacatcca ttttgtcttc
     1021 gatcagcacg gcgagccgcc gacccaggaa actcgagcag aaagcgcgct ttttttgcac
     1081 gcccaaatcg cgcaggcggc gcgcccgcgt cttcttgacg tgaaccggca cttgatcggc
     1141 gagggatgcc gccggcgtac cacgccggct cgaataggga aacacgtgaa agtaagtgag
     1201 aggcagggaa gcgaaatact ccaaagacga ttcaaattgc tgctcgcttt cgccgggaaa
     1261 accgaccatg atgtcgcttc ccagagcggc ttcgggcaaa cgttcgcgca cacgcgccag
     1321 cagatcacgg taataaccgg tgttgtaatt gcgccgcatt cttgtgaggg tctcgtcatc
     1381 tccggcttgc gcgcaaacat gcagatgtgg acaaaccatc tgagacgaag cgatcaggtc
     1441 gatcagccgc tccggcagct cgcggggatc gagtgagctc aaacgcaacc gggaaatcaa
     1501 tccgcttgcg gcgatcatct ccacgagctc ggtgagatca atcttcggtc gtaaatcctg
     1561 accatagccg ccgagatgaa ttcccgtaag cacgatttct ttgtagccgg ctccggccaa
     1621 ttgtttgacc tgctccatca cttcccgcgg ggcaacgctg cggctgagcc ctcgggccgc
     1681 tggaatgatg cagtaggtac aggaataatt gcagccttcc tgaattttca gaaatgcgcg
     1741 cgtgtgcccg ggaagggcgc gagtgccgat taccggaaca ccgcgctcgc gtttgacatc
     1801 gctcacagtg atttggggtg cagttggccg atccgcagcg atgaaccgaa tcaaatcgtc
     1861 caagcgattg agcccaacga cgtaatccac gccgggaacc cgagcgacgt cgtgtggatt
     1921 tacctgcgca taacagccgg ttactagaat cttcgcctgc ggatttcgcc gcttggcgcg
     1981 gcggaccagt tggcgcgctt cccagtccgc ccggtccgtg accgtgcacg tattgataat
     2041 gtagcactcc gcactcgcct cgaagggcac catcgagtgc tcatgttcca agcggctctg
     2101 tatcaccgcg gaatcatatt ggttgatctt gcaacccaat gtagtaattg cgacccgcat
     2161 atcctgtaag cgcgcagcat cctgcgtcct atgttttacc gtccatcctg agtagcgcga
     2221 acgcgcgtat cgaaggattt tcgactttct atggccgact gcctgctgtc tcttgtcttt
     2281 cttccttcat cgcacgttcg atccagccac cgccgaggac ctcatcactg cgataaaaaa
     2341 ccgccgcctg gcccggcgtg acgccatgaa cggtttgatg gaaaactact tcacaagagc
     2401 cgttcgcatc cgatcgaacc atgcacggaa ctgccggagc gcggtagcgg atctgtactt
     2461 cggcggcaaa ttcgacttga tcgggaagat caatccaatt gacgccgctc gcgatcaggc
     2521 cagtacagcg aagctctgcc tttttaccaa ccgttacttg cttggtcgtg tcgtcaatgt
     2581 cgattacata gagcggttcc gccgccgcaa ttcccaaccc gcgtctctgg ccgacggtta
     2641 cgctatgaat accatggtgc cggcccagga ctttacctga tcgattgatc acatcaccgc
     2701 cgcgaagttc gtccttctcg gcataggagg caaccaggtc tttgtaatcg ccaaaacaaa
     2761 tatcctggct ttccggacgc tctgccgccg gcagtcgcag acgccgcgcc cgttcccgga
     2821 cttcggtttt ttgcatctcg ccgagcggaa acagcacccg cgcgagctgc tcttgcctta
     2881 gcgcaaatag aaaataggac tgatccttgg cgcgatcggc a
//
LOCUS       DASULZ010000267         3086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1046614, whole genome shotgun sequence.
ACCESSION   DASULZ010000267 DASULZ010000000
VERSION     DASULZ010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046614"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VFY96_05595"
     CDS             <1..517
                     /locus_tag="VFY96_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605968.1"
                     /translation="VTLLQLGGMLELATALSKKLIAPATLSSPADLRARRSGAQQLVN
                     MATTRVSFPQSAIVTTRSYIQAHQDQVLNFLRAYAEGMQTMIADKVLAKHVIQKYTRE
                     TDSDILESTYRYGVDYIAPVPYPARDGIVEILKQSNHPKAKTANPDDFIDMSLVKKIE
                     ESGFFRPTSHR"
     gene            548..1684
                     /locus_tag="VFY96_05600"
     CDS             548..1684
                     /locus_tag="VFY96_05600"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5605969.1"
                     /translation="MIGDYAVFDADAHAMMCPKMWEELPATYAARRPRPVRVEDSADL
                     GRWTNGWLIEGRLLPAPFGPGAQPGNEPRRVLREFGAPESGHFSIGSITLADPKARIR
                     DLDEAGVDAQMLFPTTLYARMTSEPGFEAALFRCYNRYMARQCSFAPKRLKWGGLLPM
                     RDAPQAFEAIVEMQKLGASAAVVFGTVGDRLLSHSSFLPIWEKFASTGLPLCVHMGMS
                     YTPFEQICQNLFETHGIGMSLPAQLAFVALVGDGILDRFPSMKVAFLEFGAEWMFYMI
                     GRLGHYLPIDRHDMPIKDSLPKRSIEEYLASGRLFVAAEAEDRLLSHELAMIGEDVIV
                     CSSDIPHSEKRENSATKIIERTDLSDRQKRKILYDNTVRLFGRP"
     gene            1764..2570
                     /locus_tag="VFY96_05605"
     CDS             1764..2570
                     /locus_tag="VFY96_05605"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HEX5605970.1"
                     /translation="MSEAVTVQLPYTVDDGEVLVNESFGPKNIHGRRSGTVELKTMTL
                     LNGRVLGERLSLDEQGFVFVEHKTQVKDFFDENQLKTIYYPEIEQLIRSQSGASRVVI
                     FDHALRSGDESEREQKLIRNPVLQAHNDYTEWSGPNRLREILPGEADELLKRRFAIIQ
                     VWRAINRPIQSNPLALADARSIACEDLMVAERRYPHRVGQTYRLKFNPDHRWIYFPEM
                     RRDEAIVFKVYDSDKTRARFTAHTSFDDPATPPGAPPRQSIEMRAFAFFA"
     gene            2822..>3086
                     /locus_tag="VFY96_05610"
     CDS             2822..>3086
                     /locus_tag="VFY96_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605971.1"
                     /translation="MWKKVTLLFALMSFAGFSAGAADTTLQQVADALDVSTTRTFQFT
                     ANGTMYNLGQNNNPAAPWPRFFVKNSVRLYDFTAGAMRDEVVRM"
BASE COUNT          704 a          810 c          848 g          724 t
ORIGIN      
        1 cgtgactctg ctccagcttg gcggcatgct ggaattggca acggcgctct caaaaaagtt
       61 gatagcgccg gcgacgctct cgtcgccggc ggatttgcgt gctcgaagaa gtggcgcgca
      121 gcaattggtc aatatggcga ccactagggt ttcgtttccg caatcggcca tcgtcaccac
      181 tcgctcctac atccaagcgc atcaggacca agtcttgaat tttcttcgcg cttacgctga
      241 gggaatgcaa acaatgattg ccgataaagt gctggcaaaa cacgtgatcc agaaatatac
      301 ccgcgaaacg gattctgata ttctcgaatc gacttatcgc tacggcgtgg attacattgc
      361 gccggttcct tatcccgcga gagatggaat cgtcgaaatt cttaaacaga gcaatcatcc
      421 gaaggcaaaa accgccaatc ccgatgattt tatcgatatg agcttggtca agaaaataga
      481 agaaagcggg ttctttcgcc caacgagcca ccggtgacgc tcacaggctt atcagaaagg
      541 gaaatcgatg atcggtgact atgccgtttt cgacgccgac gctcacgcga tgatgtgtcc
      601 aaagatgtgg gaagagttac ccgcgaccta cgccgcgcgg cggccccgtc cggttcgcgt
      661 cgaagattct gccgaccttg gacgatggac gaacggctgg ttgatcgaag gtcgattgct
      721 gccggcgcct ttcggaccgg gagcgcaacc agggaatgag ccgaggcgag ttttgcggga
      781 atttggcgcg ccggagtcgg gccatttttc catcggcagt atcacgttgg cagatccgaa
      841 agcacgcatt cgtgatttgg atgaagccgg cgtcgatgcg caaatgcttt ttccgacgac
      901 attgtacgcc cggatgactt ccgagcccgg tttcgaagcg gctttgttcc gttgctacaa
      961 tcggtacatg gcgcgtcaat gctcgttcgc tcccaaacgt ttgaagtggg ggggattgtt
     1021 acccatgcgc gatgcgcctc aggcgttcga agcgattgtt gagatgcaga agctcggcgc
     1081 gagcgcggca gttgtatttg ggacggtggg tgatcgcctc ctgtcccatt ccagctttct
     1141 accgatctgg gaaaagtttg cttctacggg attgccgctt tgcgtccata tgggcatgag
     1201 ctacacgccc tttgaacaga tctgtcagaa cctttttgaa acccacggca ttggtatgag
     1261 cctgccggcg cagctggctt ttgtcgcact tgtcggcgac ggcattctcg atcggtttcc
     1321 gtcgatgaag gtggcgtttt tggagttcgg cgcggaatgg atgttttata tgatcggccg
     1381 tttggggcac tatctgccga tcgatcggca cgacatgccg atcaaagatt cactgcccaa
     1441 gcgctccatc gaagaatatt tagcctccgg ccgattgttt gttgccgcag aagcggaaga
     1501 tcgattactt tctcatgagc tggcaatgat tggcgaggac gtgatcgttt gttcttcgga
     1561 tattccacac tcggaaaagc gcgagaattc cgcaaccaag attattgaac ggacagattt
     1621 gtccgaccgg caaaaaagga aaattcttta cgataacaca gtgcgtttgt tcggcaggcc
     1681 gtgatctcga cacttacatg acccggtgaa gcttgttagt ctaacgcatc gtagagtgcg
     1741 aaatttaatg aggagccccc tccatgtctg aagcagtcac tgtccaactc ccttatacgg
     1801 tcgatgacgg cgaagttctc gtgaacgaat ccttcgggcc gaaaaatatt cacggacgaa
     1861 gatccggaac cgtggagctg aagactatga ctctgcttaa cggcagggtt ctcggcgaac
     1921 gcctttcact ggatgaacaa ggatttgtgt ttgttgaaca caagacgcag gtgaaggact
     1981 tttttgatga gaatcaattg aagacgatct actatcctga aatcgagcag ttgatccgga
     2041 gtcagtcggg cgcttcgcgc gtggttatat tcgatcacgc attgcgctca ggtgacgaga
     2101 gcgaacgcga gcaaaagctg atccgaaacc cagtgctaca ggcacacaat gattatacag
     2161 aatggtccgg gcccaatagg ctccgggaaa ttcttcctgg cgaagcggac gaactgctga
     2221 agcgccgctt tgccatcata caggtatggc gggcgatcaa ccgcccgatc caatcgaatc
     2281 cgctggcgct ggccgatgcc agaagcattg cctgcgagga cctaatggtc gccgagcggc
     2341 gatatccaca ccgggtcgga cagacctatc gactcaaatt caaccccgac catcgttgga
     2401 tctattttcc cgagatgcgc cgtgacgaag cgatcgtctt taaggtttac gactcggaca
     2461 agacgcgggc gagattcacg gcgcacacct cattcgatga tccagcgact ccgccgggtg
     2521 cgccgccacg acagagtatc gagatgcggg cgttcgcgtt ctttgcctga tcacctgttt
     2581 ccgctcgaac cttttctcgc agagctgtct tcgaccgatc tggctttaag gcggctttcc
     2641 gaccgcgtcc tctactactg ttctcgtcca agttgatcaa atcagtaggc tctctgtgga
     2701 cacgttcttt ctcagccagc tcttcaggca tatggcattg acagcgccga agaccatcgg
     2761 gtagtctgcg cgccaaattg acacacgacc cgtgatctca cgagacccat caggagaatt
     2821 tatgtggaag aaggtaacgc tgcttttcgc tctcatgtca tttgcgggtt tcagtgcagg
     2881 cgccgcggac acgacattac aacaagtcgc cgatgcgctc gacgtatcaa cgaccagaac
     2941 gtttcaattc actgcaaacg gcacgatgta caatcttggc cagaataata atccggctgc
     3001 gccgtggccg aggttcttcg tcaaaaattc cgtgcggctc tatgatttca cggccggcgc
     3061 aatgcgtgat gaagtggtgc gtatgg
//
LOCUS       DASULZ010000268         3013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1149389, whole genome shotgun sequence.
ACCESSION   DASULZ010000268 DASULZ010000000
VERSION     DASULZ010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149389"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(335..1744)
                     /locus_tag="VFY96_05615"
     CDS             complement(335..1744)
                     /locus_tag="VFY96_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018622546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5605972.1"
                     /translation="MLASSTILELKSRFHGELILPDDRQYETARAVFNAAIDRRPALI
                     ARCFTSADVIEAVNFARRENLLLAVRGTGHNVAGFAVCDDGIMIDLSAMKGICVDAPA
                     RSVRVEAGCNWGEVNDALQPDGLAATGGFVSVTGVSGLTLGGGLGWLVRKHGLALDNL
                     LSAEVVLAEGRVVTASARENEDLFWAIRGGGGNFGVVTSFEFRVHPVGTVLAGIVLHP
                     AAAAAGAVRRWRDLEATAPDESAQGALLFHFTEDAPVPVPLRGAAVVGLGGVYAGDLE
                     KGEKILRPLREYGPPLADMFRPMPYNEAQRMADFLWPPGLHSYWKSSYLTALSDAAID
                     VFVDFFARVPSKRTVIVLEHNGDSAWDRVPDAETAFGHRSWAYNFVVTSAWSDPKDAQ
                     RNIGWTSELFDALRPYLARGAYVNYLGGDEGADGIQAAYGAKLARLAALKRKFDPTNL
                     FRMNHNIVPAEVGAAESSI"
     gene            2003..2986
                     /locus_tag="VFY96_05620"
     CDS             2003..2986
                     /locus_tag="VFY96_05620"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5605973.1"
                     /translation="MQKSILLTALMLCLTATSSWAQSHTKLNVIYPAISGINAPLWYT
                     TEQKIFPKYGLDVELIYIPTALQVTRVVLTGDSQIAFSGGAPVVSAVLSGADLVIIGG
                     VANVPAFYMMALPEIKTVGDLKGKTVGVTRFGSSTDFALRYLVQKNGFNPDKDLNILQ
                     LGGMPELAAALSKRLIVAAPLSAPTHIRARAAGAVPLLDMAKAGVYFPHTAVITRRGY
                     LKTNRDTGLNFFRGYCEGLQRFIQDKNTAKKVIQKYTRDTADDIIEATYQYAVDYVVR
                     PPYPNREGIVETLKLSPNPEAKKANPDQFLDSSIVKTLEDEGFLRQIGMQK"
BASE COUNT          655 a          904 c          851 g          603 t
ORIGIN      
        1 gtaataaaac gtgaaaaagt cttctccgag attcgcagac ctatattctc attactagag
       61 aaaggtccgc tcgcggacag ctgcacatac ctatcgtacg agagccttcg cgaactcgcc
      121 tccgacaaac atctcgcata cctttcagac accgtactcg atgaatatgc ggaagaggcg
      181 gaatagttaa tcaccaaagt tattgattat cgtactacga acgccgacca gctcaatcat
      241 ctcagtgtct cgcggcactt gccgaatcac taattatctc atgggaatgc cgtaatcgct
      301 gtgaaggatc tggagatcaa ctctttactt ttcactatat gctggattca gcggcgccaa
      361 cctcggccgg cacgatgttg tggttcatgc ggaaaaggtt ggttgggtcg aatttccgct
      421 tgagcgcggc gaggcgcgcc agcttcgccc cgtatgccgc ctggattcca tcggctcctt
      481 cgtcgccgcc tagataattc acgtaggccc cccgggcgag ataggggcga agcgcatcga
      541 aaagctcgct cgtccagccg atgttccgct gcgcgtcctt cggatcggac caggcggagg
      601 tcacgacgaa attgtaggcc caggagcgat ggccgaaagc cgtctcggca tccggcacgc
      661 ggtcccaagc gctgtcgccg ttgtgctcca acacgatcac cgttcgtttc gatggaactc
      721 gcgcaaagaa atcgacaaaa acatcgatcg ctgcatcgct gagcgccgtc aggtagctcg
      781 atttccagta actgtgcagt cccggcggcc agagaaaatc cgccatgcgc tgcgcctcgt
      841 tgtacggcat cggccggaac atgtcggcga gcggaggacc gtattcgcgc aacggacgca
      901 ggattttttc gcctttttcc agatcgccgg catagacgcc gccgaggccg acgacggccg
      961 ccccgcgcag cggcactgga accggcgcgt cctcggtgaa atgaaacagc agcgcaccct
     1021 gagcggattc gtcgggcgcc gtggcttcga ggtctcgcca tctgcgcacg gctcccgctg
     1081 cggcggccgc cggatgaagc acgattcccg ccagcaccgt gccgacagga tggacgcgga
     1141 actcgaaaga agtcaccacg ccgaagttgc cgccgccgcc gcgaattgcc cagaagagat
     1201 cctcgttctc gcgcgcgctg gcggtgacaa cgcgaccctc ggcgagcacc acctcggcgg
     1261 aaagcaggtt atcgagcgcg agaccatgtt tgcgcacgag ccagccgagg ccgcccccaa
     1321 gcgtgaggcc cgaaactccg gtaaccgaaa cgaagcctcc ggtcgcagcg agaccgtctg
     1381 gttgaagcgc atcgttaacc tcgccccaat tgcatccggc ctcgacgcga acggaacgcg
     1441 cgggggcgtc aacgcaaatg cccttcatcg ccgaaaggtc gatcatgata ccgtcatcac
     1501 agacggcaaa accggcgacg ttgtggccgg ttccgcggac ggcaagaagc aggttctccc
     1561 ggcgcgcgaa gttcaccgcc tcgatgacgt cggccgaggt gaaacaccgg gcaatgagcg
     1621 ccggccgccg atcgatggcg gcgttgaaaa ccgcgcgcgc tgtttcgtac tgccgatcgt
     1681 ccgggagaat cagttcgccg tgaaaacgag atttaagttc tagaatggta gaagacgcaa
     1741 gcatggtgcc ctccttttgc tgcgggtcgt tcgttgcacg agcggcgttg tgacgcgatc
     1801 gaacgagata ttgatttcgt atacccgatc gacattcggc tcaagcagtt actgggtttt
     1861 cttcagtgat cgaactgcga cttaaacttt tcgccggggt cttcgtcgtc gacgcgtggt
     1921 gtgaaccaaa acataattaa agcggggctt ggactccgca cgttcttgac tcggcagcgg
     1981 tgcactcgta tggtgccgaa ccatgcagaa atccatttta ctcacggcat tgatgctctg
     2041 tctgacggcg acttcatcgt gggcgcagag tcacacgaaa ctcaacgtga tctatcctgc
     2101 catcagcggc atcaatgcgc cgctttggta taccaccgag cagaagatct ttccgaagta
     2161 cgggctcgac gtggagctga tctatattcc caccgcgctg caagttactc gcgtggttct
     2221 gaccggcgac tcgcagatcg ctttttccgg cggcgcccca gtggttagtg ccgttctcag
     2281 cggcgccgac ctggtcatca tcggcggcgt ggcgaacgtg cccgcgtttt acatgatggc
     2341 tcttcctgag atcaaaaccg tcggcgattt gaaaggcaaa accgtggggg tcacccgatt
     2401 cggctcgtcg accgatttcg cactgcgtta tcttgtgcaa aaaaacggct ttaatcccga
     2461 caaggatctc aacatcctcc agctcggcgg catgccggag ctcgccgcgg cgctctcgaa
     2521 gcggctgatc gtcgccgcgc ctttgtcggc gccgacccac attcgggcgc gcgccgccgg
     2581 cgctgtgccg ctcctcgata tggcgaaagc gggggtttat tttccgcaca cggccgttat
     2641 cacccggcgg ggttacctga aaaccaaccg cgacacgggg ctgaattttt tccggggcta
     2701 ttgcgaaggt ctgcagcgtt ttattcagga taagaacact gcaaagaaag ttatccagaa
     2761 atacacgcgc gacaccgcgg atgatatcat cgaagcgact tatcaatatg cggtcgatta
     2821 cgtcgtccgg ccgccctatc ccaatcgcga gggcatcgtc gagacgctca agctaagccc
     2881 aaatcccgaa gcgaaaaaag ccaacccgga tcaattcctc gatagcagca tcgtaaaaac
     2941 gctcgaagat gagggctttc tacgccagat cggcatgcag aaatgaacga cacccatggt
     3001 tcgattttcg att
//
LOCUS       DASULZ010000269         4011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_439085, whole genome shotgun sequence.
ACCESSION   DASULZ010000269 DASULZ010000000
VERSION     DASULZ010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439085"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VFY96_05625"
     CDS             <1..278
                     /locus_tag="VFY96_05625"
                     /inference="COORDINATES: protein motif:HMM:NF018349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1152 domain-containing protein"
                     /protein_id="HEX5605974.1"
                     /translation="TEASAIPLRCLNGGWGEQKIRGGERSVRLTPLTALTFFVSTRVV
                     FETLSRPAQAVQRSGSLAEANQALHALGIKTELDLEFERYHQAALAR"
     gene            275..1540
                     /locus_tag="VFY96_05630"
     CDS             275..1540
                     /locus_tag="VFY96_05630"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5605975.1"
                     /translation="MKLIGENFQRDVLVLMASRGVRAFAFSYLSVVFAIYLAQLGFST
                     VTVGLVFSTGYATSALLTALWGYLADRYGRKKILILLAVLMIISNLLYLFFSHLIFIL
                     IAESIAAVGVGSSGAGGQGGGPFNPVEQALLAEKCTPENRNRIFATNACVGSIMGSLG
                     ALVSALPQYLQQRHGWEIVTSYKPLFAMTVLFSIGIIFAYASIDEHHVPRQRKAAERQ
                     AVKRSRDFGIVGKLAMLGVVDNLGAGLVSPLLSYWFFLRFGVELKSLGLTFFLSYILA
                     ACSFLIAPTVARHFGVVKTMAVSHGMASVLNILLPFAPTFSLAAGITVARSFLAYMDS
                     PLRSSFVMGVVRPEDRGSAAGITTLSRQVPVAIRPTISAYLMHSFSLNVPIFLGGFLQ
                     LLNDFSFYFLFRDVKPPEEQTAKSQPVTA"
     gene            1542..2807
                     /locus_tag="VFY96_05635"
     CDS             1542..2807
                     /locus_tag="VFY96_05635"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5605976.1"
                     /translation="MKILRELHRDAKLLMAARGIRAFAFSYLNVVFAIYLDRLGFAPV
                     MIGMIFTVAYLSGALLTAVWGYLSDRVGRRKILMLLALLTICSNFILIFFTSLLFILA
                     AVVIANVGAGGSGGGGSGGGPFNPVEEALLAEKCRPENRNQIFAINSCVGSIMGSLGA
                     LASGIPQFLEEDRGWSSVASYKPLFLLTIGFSIALLFVYRGIGEQHQPRRTERKMSKS
                     TGAFVTKMSALSIVDNFGAGMAGALVPYWFFLRFGVELKSLGIIFFASYFLAALSFLS
                     APLIARRIGVVRTMAFSHGFASLIWLCLPFAPILNIAAALIVTRSFFAYMDNPLRASF
                     TMAMVQANERGSAAGVTSLARIVPFGISPTISTYLMQTVSLTLPLFIGGGLQLINDIA
                     FFLMFRHVRPPEELAVSYTKSSTAEELSG"
     gene            2804..3205
                     /locus_tag="VFY96_05640"
     CDS             2804..3205
                     /locus_tag="VFY96_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013401650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HEX5605977.1"
                     /translation="MKRPREETGAVSFLQRIAEQRILEAQREGAFDNLPGKGKPLELE
                     DLSWVPEDLRIGYHVLKNAHVLPPEVELLKDIHSLEDLLKHVEDETERRSLAKSIQWK
                     MVRLDMLKRRSLDLNAVRTYGRKLVAKFQLK"
     gene            3300..3809
                     /locus_tag="VFY96_05645"
     CDS             3300..3809
                     /locus_tag="VFY96_05645"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5605978.1"
                     /translation="MAEEQSPIKIKKIGHVVLSVSDIERSTKFWTEVMGFKFSDKNEV
                     GMMFFRNGTDHHTVALMQATEKNELPKRGEVGFDHMALEVATVSELFKIRDFLKSKGV
                     TIFYEGRRGPGGNPGLEFYDPDGYKIEIYASMDQIGTDGRSRPANEWKRATTLEEAVA
                     NPLPNTDYH"
BASE COUNT          812 a         1098 c         1113 g          988 t
ORIGIN      
        1 ccacggaagc gagcgcgatt ccgctgcgct gcctgaacgg cggctggggc gaacaaaaga
       61 tccgcggtgg tgagagaagc gttagactca cgccgcttac ggccctcacc ttcttcgttt
      121 ccaccagggt ggtcttcgaa acgctgtcac ggccggccca ggctgtgcag cgaagcggca
      181 gtttggccga agccaaccag gcgttgcacg cgctcgggat caaaaccgaa ctcgacctgg
      241 aattcgagag atatcaccag gccgccttgg ctcgatgaaa ctgatcggcg aaaactttca
      301 gcgagacgtg ctggtgctga tggcttcgcg gggggttcgc gcatttgcct tttcctatct
      361 cagtgtggtt ttcgccatct atctcgccca actcggtttt tcgacagtga cggtgggatt
      421 ggttttttcc acgggctacg ccacgagcgc gctgcttacg gcgctatggg ggtacttggc
      481 cgaccgctac ggccgtaaaa aaatattaat actcctcgcg gttttgatga ttatttccaa
      541 tcttctctat ctctttttca gccatcttat attcattttg atcgccgaga gcattgccgc
      601 ggtcggcgtt ggcagctccg gcgccggggg ccagggtggc ggccccttta accccgtcga
      661 acaagcactg ctcgccgaga aatgcacgcc ggaaaataga aatcgcattt tcgccaccaa
      721 tgcctgtgtg ggatcgatca tggggtccct cggcgcactg gtgagcgcgc tgccccagta
      781 tttacagcag cgacacggtt gggaaatcgt cacctcatac aagccgctct tcgcgatgac
      841 tgttttattc agcatcggga tcatttttgc ttacgcgagc atcgacgagc accatgttcc
      901 gcgacaaaga aaagcagctg agaggcaagc ggtaaagcgc tcccgcgatt tcggcattgt
      961 cggtaaactg gcgatgctcg gcgtcgtcga taacctcggc gccggcctgg tcagcccgct
     1021 cctgtcgtac tggtttttct tgcgcttcgg cgtagagttg aaatctctcg gtttgacgtt
     1081 ctttctttcc tacattctcg cggcctgttc attcctgatt gcgccaaccg tcgcgcgcca
     1141 ttttggcgtg gtcaagacta tggcggtttc tcacggtatg gcttcggtgc tcaacatcct
     1201 gttgcctttt gcgccgactt tctcgctggc ggccgggata accgtggcgc gttcctttct
     1261 cgcctacatg gacagtccgc tcaggagctc gttcgtcatg ggtgtggtcc gtcccgagga
     1321 tcgaggctcc gccgctggaa tcacaaccct ttcacgccaa gtgccggtcg ccatccgtcc
     1381 gacgatttct gcttatttaa tgcattcgtt ctcgttgaat gtgccgattt ttctcggcgg
     1441 cttcctgcag ctgctgaacg acttcagctt ttatttcctg ttccgcgatg tgaagccccc
     1501 ggaagaacag accgcaaaat cgcaaccggt cacggcgtag catgaaaata ttgcgcgagt
     1561 tgcatcggga cgcaaaattg ttgatggcgg cgcgcggcat ccgcgccttc gcgttttcct
     1621 acttaaacgt cgtcttcgcg atttacctcg accggctcgg ctttgcaccc gtcatgatcg
     1681 gcatgatttt tacggttgcg tatctcagcg gcgcgctatt gacggcggtc tggggatact
     1741 tatcggatcg cgtcggccgg cgaaaaatat tgatgctgct cgcgcttctc acgatctgct
     1801 ccaatttcat cctgattttc tttaccagcc tattgttcat cttggccgcg gtggtaattg
     1861 caaacgtcgg cgcgggcggc tcgggcggcg gcggctccgg cggtggacca ttcaatccgg
     1921 tcgaagaagc cttgctggct gaaaaatgcc ggccggagaa ccgcaatcag atctttgcca
     1981 tcaactcctg cgtgggatcg atcatgggat ccctaggagc gcttgccagt ggtataccgc
     2041 agttcctcga ggaggaccgc ggctggagct ctgtcgcctc gtacaagccg ctttttttgt
     2101 tgaccatcgg gttcagtatc gcactgcttt tcgtctaccg cggcatcggc gagcagcatc
     2161 agccgcgcag gactgaacgg aagatgtcga aaagcacggg agcgttcgtt accaaaatgt
     2221 ccgctctctc gatcgttgat aattttggcg ccggtatggc gggtgcgttg gtgccgtact
     2281 ggttttttct ccggtttggc gttgaactaa agtctctggg cattattttt ttcgcgtctt
     2341 attttctcgc ggcgctctcg tttctaagcg cgcccttgat cgcgcgcaga atcggcgtgg
     2401 tgcgcacgat ggcattctct cacggctttg cctccttgat ttggctttgc ctccccttcg
     2461 cgccgatttt gaacatcgcc gccgcgttga tcgtaacgcg atcattcttt gcgtatatgg
     2521 ataacccctt gcgggcttct ttcacaatgg cgatggtcca agccaatgag cgtggttcgg
     2581 ctgccggcgt gacgagcctc gcgcgcatcg ttccgttcgg catcagtccg acaatatcga
     2641 cctacttgat gcaaacggtt tccctcactc ttccattgtt catcggtggc gggttacaat
     2701 tgatcaacga catagctttt tttctgatgt tccgtcacgt gcgaccgccg gaagagctgg
     2761 cggtgagcta cacaaaatcc tcaactgcgg aagaattaag cggatgaaga gacccaggga
     2821 agagaccgga gctgtctcgt ttctgcagcg catcgccgag cagcggattc tcgaagctca
     2881 gcgcgagggt gccttcgaca atttgccggg aaagggaaaa ccgctcgagc tggaggattt
     2941 aagctgggtc ccggaggacc tgaggatcgg ctatcacgtg ctcaagaatg cccatgttct
     3001 gcctcccgag gttgaactac tcaaagatat tcacagcctc gaagacctgc tcaagcacgt
     3061 tgaagatgaa accgagcgcc gctctctcgc caaaagcatt cagtggaaaa tggtccgcct
     3121 cgacatgctc aaacgccgct cgttggatct taacgcggtg cgtacttacg gccgtaagct
     3181 ggtcgcgaaa tttcagctaa agtaggttcg ctgcgaccgg tacgtttaat tttgccttga
     3241 ggaatcatcc gtgctagaaa ttctttgctt tagcagtgac ttgggaaagg aggaagctta
     3301 tggctgaaga acaatcgccg atcaaaatca aaaaaatcgg ccacgtggtg ctgagcgtca
     3361 gcgatatcga gcgtagcaca aaattttgga ccgaggtgat gggatttaag ttttccgaca
     3421 agaatgaggt cgggatgatg tttttccgta acggcaccga ccatcatacc gtggcgttga
     3481 tgcaggcaac ggaaaaaaac gagctgccca agcgcggcga ggtcgggttt gatcacatgg
     3541 cgctagaagt cgcgacggtg tcggagcttt tcaagatccg cgattttctc aagtcaaagg
     3601 gtgtaacgat cttctatgaa ggacggcgcg ggcccggtgg caatcctggt ctcgaattct
     3661 atgatccgga cggctacaaa atcgagatct atgcctccat ggatcaaatc ggcacggacg
     3721 gccgcagccg accggcaaac gaatggaagc gtgccaccac tttggaagaa gccgtcgcaa
     3781 atcctttgcc taacacagat taccactgac ttcgcatccc taaacagcgc acggttttgc
     3841 ttctaggggg cgcagcatgc tgcgccctag aagcatttaa cagttcatcc ggacggcgca
     3901 attgtcatga aagcgagaga tctttcattt tttcgcggat gcgaagaaag atttcccgcc
     3961 ggtgcctcga aatgacacga tcaaacggtg agttacgact gggagactcg a
//
LOCUS       DASULZ010000270         2394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1352707, whole genome shotgun sequence.
ACCESSION   DASULZ010000270 DASULZ010000000
VERSION     DASULZ010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352707"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VFY96_05650"
     CDS             <1..183
                     /locus_tag="VFY96_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5605979.1"
                     /translation="LAAKARALLAGRPNVSFDDIRTSLLPSLRHRLILNLEADAEGVT
                     AEKILTEVAAQVPEME"
     gene            316..1236
                     /locus_tag="VFY96_05655"
     CDS             316..1236
                     /locus_tag="VFY96_05655"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5605980.1"
                     /translation="MNTTRPEPENRNRTSENFFDSDFLRKLERLHLIAKRLSWAGAKG
                     EHAVSRKGFSLEFSDYRRYQRGDDLRYIDWNVYRRLERLLLKVFTAEEEMNIYLLVDT
                     SRSMAEGKPAKIDYAKKIAAALGYIGLKNLDRVGGASFSTQIAKPLTLGRGRKQVLSL
                     FRFLANLSASGQTNLRAAIHSFTNLFPHPGFVIIVSDLFDETGWRRALEELTAKRYQI
                     LVIHIVDTEETQPKAWGDVALMDVEAGRERKFFLDADLVRRFQTEIERYFNEIETTCA
                     RRHIDYLRTTTQVPFDEFVLQTLRQVGSVA"
     gene            1240..>2394
                     /locus_tag="VFY96_05660"
     CDS             1240..>2394
                     /locus_tag="VFY96_05660"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5605981.1"
                     /translation="MLWSLPSALFLLAGAIPLILFLHSLKPRGLKVGTTTMFLWERIL
                     RERPLGTRLGWLLRHNLLLLLQILAALALIIALADPALLHFGSSAGDLIVVIDLSASM
                     KATGRSGSRFDTARREFDKLIDNMSGAQKMMVIGAGVQPRLLMPFTAEKRRLRDLARG
                     LEATDAPGRVKETILFAHTFLKRDSADRIVIISDGAFKSAEEFTKPAAHYRFVSVAEG
                     SENIAIVGFEARVHSDRSAPAEIMVHLRNFTAKAVRLPLAVTLGEKLLAREEIEIGAG
                     ERRVMIYPFTGELRGILTARLEAKDDFATDNVAYLALTEQAPLRLLYVGPGNPYLQQV
                     LRLFPHVQLSTAIGWDEGITHGAEPFDLVIFDRVAAPALKQGNFILINSVS"
BASE COUNT          503 a          665 c          698 g          528 t
ORIGIN      
        1 ttggcggcaa aggcccgagc gctgttggct ggcagaccca atgtctcgtt cgacgatatt
       61 cgcacttcac tgctgccgag tttgcggcat cgcctcattc tcaatctcga agccgatgcg
      121 gaaggcgtta cggcggaaaa gattttaacc gaagtggcgg cgcaagtgcc ggaaatggaa
      181 taattcatga gtctcgcgtc tcgcgttgcg agtttaagat tcgtgccggc gcgaatcagt
      241 gacttgtttg ggcgaattgt tgtccgcgcg cggggaaaga cctctcgctc tgctcgggag
      301 gacaacgatg gggcggtgaa cacaacccga cctgaacctg aaaatcgaaa ccggacttcc
      361 gagaatttct ttgacagcga ttttttacgc aagctggaac ggcttcacct gatcgccaag
      421 cgcttaagct gggcgggcgc caagggggag cacgccgttt cacgcaaggg cttcagcttg
      481 gaattttccg actatcgccg ctatcagcgc ggcgatgatc tacgttacat cgactggaac
      541 gtttatcgcc gtctcgagcg gttgctactc aaggtcttta ccgccgagga agaaatgaac
      601 atttatctcc tggtcgatac cagccggtcg atggcggaag gtaaaccagc gaaaatcgat
      661 tatgcaaaga aaatcgccgc tgccctcggg tatatcggtc ttaaaaatct ggatcgagtc
      721 ggtggtgctt cgttttcaac gcagatcgcc aaaccgctca ccttggggcg gggccgtaag
      781 caggtgctca gcctgtttcg ttttctggcg aatctttctg ccagcggcca aaccaacttg
      841 cgcgccgcaa ttcattcctt cacgaacctg tttccgcatc cgggtttcgt gatcatcgtg
      901 agcgatttgt tcgacgaaac cgggtggcgc agagccttgg aagaattgac ggcgaagcgc
      961 taccagatcc tggttatcca tattgtcgat acagaagaaa ctcaaccgaa agcgtggggc
     1021 gatgtcgccc tgatggacgt tgaagcgggc cgggaacgaa aattctttct cgatgccgat
     1081 ctggtgcggc gctttcaaac cgagatcgag cgatatttca atgagatcga aacgacctgc
     1141 gcgcgccggc acatcgacta cttgcgcacc accacccaag tgccgtttga tgaattcgtg
     1201 ctacagacgc tgcgccaggt gggcagcgtg gcttgaacta tgctttggag cctgccgtcg
     1261 gccttattcc tgctcgccgg cgcaatcccg ctaatcttat ttctccatag cctcaaaccc
     1321 aggggcctaa aagtcggtac gacgacgatg ttcctttggg agcgcatcct cagagaacgc
     1381 ccgctcggca cccggctcgg ctggctgcta cgccacaatc ttttgttgct cctgcagatt
     1441 cttgcggcac ttgcgctgat catcgcactc gcggacccag cactgcttca cttcggctcc
     1501 agtgccggcg atctcatcgt cgtgatcgat ctgagcgcga gcatgaaagc caccggccgg
     1561 agcggctcgc gcttcgatac ggcgcggcgg gaattcgaca agctgatcga caacatgtcc
     1621 ggcgcgcaaa aaatgatggt gatcggcgcc ggcgttcagc cgcgcctgtt gatgccgttt
     1681 acggctgaga agcggcgctt gcgagatctg gcgcgcgggc tggaagcgac tgatgcaccg
     1741 gggcgagtca aggaaacgat ccttttcgcc catacttttt tgaagcgtga tagcgcggat
     1801 cggattgtga tcatcagcga tggcgccttc aagagcgccg aagagtttac caagccggcg
     1861 gcccattacc gcttcgtcag cgttgctgag ggtagtgaaa atatcgctat tgtcgggttc
     1921 gaagcaaggg ttcattcgga tcgaagcgcg cctgcggaga tcatggtgca cctccgcaat
     1981 ttcaccgcca aagctgtccg actaccattg gccgtgaccc tcggtgagaa gctgctcgcg
     2041 cgtgaagaga tagaaatcgg cgccggcgag cgacgggtga tgatctatcc tttcacgggc
     2101 gagctcagag ggattttgac cgcccgattg gaagccaagg acgattttgc aacggacaac
     2161 gtcgcctact tggcgctcac ggagcaggcg cccttacgac ttctctacgt cggcccggga
     2221 aatccttacc tgcaacaggt gctgcgcctg ttcccgcatg ttcagctaag taccgcgatt
     2281 ggttgggacg aggggataac gcacggcgcg gagccctttg acctggtaat cttcgatcgc
     2341 gtcgcggctc cggcgctgaa gcaggggaat ttcatcctga tcaatagcgt cagt
//
LOCUS       DASULZ010000271         5928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1149650, whole genome shotgun sequence.
ACCESSION   DASULZ010000271 DASULZ010000000
VERSION     DASULZ010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5928
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149650"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            421..1464
                     /locus_tag="VFY96_05665"
     CDS             421..1464
                     /locus_tag="VFY96_05665"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5605982.1"
                     /translation="MKLLTLIVLLMLAIPSFAGAQTRKPRTLDELATYTGPDRQQILL
                     EGAKAEGKIVWYTSLSGVYRELVEAFKRKYPAVAIDVYRGGSTDLAPRLINEAQAGRF
                     VADALESTPGLLMMLREGGLLKPYTSPELAPYPDEAKTKFDATRIYWVTDREAYLGFG
                     YNTRMIAPAEVPKNFQDLLRPELKGKLAVTTESSTSRVIGTMIKLKGEEFVKRLQTQE
                     IRLFKASSAGFLDLIAAGEVAGSFVVFRNQVAVMKERKAPVEWVALDVAPANAGGSAI
                     IANAPHPHAALLFTDFVIGAEGQKLMEQFRYGVAWKDYPFKREYPERGMTSAEYEKAE
                     DRWLQLTRAITKR"
     gene            complement(1655..2536)
                     /locus_tag="VFY96_05670"
     CDS             complement(1655..2536)
                     /locus_tag="VFY96_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5605983.1"
                     /translation="MPDYKNILYQKQRSGVLITLNRPKELNALNEQLMSELDQALAEA
                     ENDPEIRGLILTGANGAFSVGEDISEDNDPPTAWPYGIPDGTSLNQVYNRFRDADRKD
                     ILQRQLNRWLYSKPIVAAVSGWCLGAGASLALTCHITIAADDAVFGQPQVRHAANTDF
                     IWVALAGFKNALRYSLTGDHVDAKEALRIGLINQVVPKAELLETCFKYVERIAHVPPE
                     TVKINLHISTQGMEMMGLRKAWALNAELAAMARLSKREEFNKRLEEARRQGGIEAFLR
                     ERDAPFQPEPFGPKAKK"
     gene            complement(2608..3486)
                     /locus_tag="VFY96_05675"
     CDS             complement(2608..3486)
                     /locus_tag="VFY96_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5605984.1"
                     /translation="MPEYQEILYEKKRGGALITLNRPDAMNAISRSMIKELHQALDEV
                     EEDNEVRAIVLTGAGRAFSAGMDQGNRAGRRRDIHWPYGIVHGQTAAEVIDSWRIDPR
                     NFRRMWEFGKPIIGAINGWAMGAGSWLSLYTHITLASENAVFAQPEVRHGSNTSFMWT
                     LLGGYKNALRYGLTGDHIDANEALRIGLVVKVVPADQLLDECFKIVERIAKVPPETVK
                     INLQVATLGLDMMGLRDALTYDKQMSAPAHVMLREELRKPLDDARANKGIREYLQMRD
                     GPFQPEPFGPRAKKKS"
     gene            complement(3761..>5928)
                     /locus_tag="VFY96_05680"
     CDS             complement(3761..>5928)
                     /locus_tag="VFY96_05680"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX5605985.1"
                     /translation="SQNNDTSWRDLSPLMNPRSVAIVGASQRTDALPNREPRGTRVIR
                     NLKNFGYPGRIIAINPKYREVMDFPCYPDLAAIPEPVDCVVLAVPNRSVPDLLESAAD
                     AGVRAAVVFAAGFAETGAEGKHRQARLEALSRERGFLICGPNCYGVLNVFGKAPLFAS
                     AIPPGFLAGPVALVSQSGGLSTTIANALMLNRHVGLSYIVSCGNQSGATFEEYFNYFI
                     EDENTRVIAGFVEGFKRAETLLAVARKAVEHKKPLIILKGGRSEVSKRAAATHSGSLA
                     GAAEVVDAAFRQGGIVSVRSLNELIDAVSVFSCERFLKLYGGGRRIGVLSGSGGECTL
                     VSDAAANVGLEIPELTKPTKLQLQQAVADFGNMNNPLDGTGAMYDDPNIFPRLLQGLI
                     DDSNIDVVTINLEANDPRPKELKSGNRFAAAIEKAASSSTKPITCFSSVVGGPVDPEI
                     LLPLRAAGVPLMEGAECATSIIQKLAEYYEFKKSSSNQTAAPTVEQLDKTLSSGIMPA
                     EAAFQLFQQYGIPTAPTMLARSVDEAALAAERVGFPVVLKIESPDITHKSDVGGVALG
                     LANANEVRVAYERMHDEVSQRAPSAKITGVIVQRMTGVGVEMILGIKRDPLFGPVVLC
                     GFGGILVELLKDVVVGIPPLSFEQARDMIARLRGFPILAGVRGKPPADVDSLCHAIVS
                     LSRLAMSLGDSIAGLDINPLIVLPKGQGVIAVDAVVEIK"
BASE COUNT         1249 a         1648 c         1689 g         1342 t
ORIGIN      
        1 tgaaaatgtg ttttacgcct tcgttcttta gacattgagc taccgcatgg ccgccgagca
       61 tagattctcc gtttcactac gtgaaatgat cgagtcgctt agatgacgat gaacatcgtc
      121 gtcaactgcc ctatagtccg attcgcggtt tgggtcaacg tttcgatgcc ggtcctgctt
      181 cagcaaagtg aagatttctc atggactttg cacgtggtcg aaagtcctgg attccgggtc
      241 gcacagctat cgccagcttg cccggaatga cgtcctgtgt ggcggattcc tttgcatgca
      301 acaatttcgt agagcgattc ttgcgcattg gctgcacgac cagcacggcc ctgttgatcg
      361 agttcttcca aacatgataa ctcggacgag ctaatgaaga cgtgcggcga ggagaaacga
      421 atgaaattgt tgactctgat cgtgctctta atgctagcca taccttcgtt cgcgggagca
      481 caaacgcgaa aaccacggac gcttgatgag ctcgccacgt acacgggtcc ggatcgccag
      541 cagattctcc tcgagggcgc caaggcagaa ggtaagattg tctggtacac ctcactttcc
      601 ggtgtttacc gcgagctggt ggaggcgttc aaaagaaaat atccggctgt cgcgatcgat
      661 gtttatcgcg gcggcagcac ggacctggcg ccgcgactca tcaatgaagc gcaagcgggg
      721 cgttttgttg ccgacgcact ggagagcacg ccgggtttgt tgatgatgct gcgcgagggc
      781 ggactgctaa agccttatac ctcgcccgag ctcgcaccat atcctgatga agccaaaacc
      841 aagtttgacg cgacgcgaat ctattgggtg accgatcgcg aggcctattt gggcttcggg
      901 tacaatacgc gaatgattgc cccggcggag gtaccgaaga atttccagga tcttttgcgg
      961 ccggagctca aaggcaaatt ggcggtaaca acggaaagct cgaccagtcg cgtgatcggt
     1021 acgatgatca agttgaaagg cgaggaattc gtcaagcggc tccagacgca ggagattcgc
     1081 ttgttcaaag cgtcgagcgc gggatttctc gacctcatcg ccgccggcga agtcgctggc
     1141 tcctttgtcg tgtttcgcaa ccaggtggcg gtgatgaaag agcgcaaggc gccggtggaa
     1201 tgggttgcgc tcgatgtggc gccggccaac gccggtggtt ccgcgattat tgccaatgcg
     1261 cctcatccgc atgccgcgct gctcttcacc gattttgtga tcggcgcgga agggcagaag
     1321 ctgatggagc agtttcgcta cggtgtggcc tggaaagatt atcccttcaa acgggaatac
     1381 cccgaacgcg gcatgaccag cgccgaatac gagaaagcag aggaccgttg gttgcagctg
     1441 acgagagcaa tcacgaagag gtaaaagggt tcagttccgg aaagacttct cacctcgttc
     1501 gagatgacca atcctctaga atgtgcattc cgagcagagc gaggaatctt tctcgaatcc
     1561 aacaatcagt cgcaacattg atttcgagtg acggagtact ggagtgatga gtttcggacg
     1621 atactccagg aatttaatac tccatcgctc cgatttattt tttcgccttc ggtccgaacg
     1681 gctccggttg aaacggcgcg tcgcgctcgc gtaagaatgc ctcgatcccg ccttgccgtc
     1741 tcgcttcttc caagcgcttg ttgaactcct cgcgtttgct caggcgcgcc atcgccgcaa
     1801 gctccgcgtt cagcgcccag gctttgcgca atcccatcat ttccatacct tgtgttgaga
     1861 tgtgcaagtt aatctttacc gtctcgggcg gcacgtgcgc gatgcgctcg acatacttaa
     1921 aacaggtttc caatagttcg gctttgggca cgacttgatt gatcaaaccg atgcgcagcg
     1981 cctcctttgc gtcgacatga tcgccggtaa gggagtagcg cagcgcgttt ttgaagcctg
     2041 ccaaggcgac ccagataaag tcggtgttgg ccgcgtgtct tacttgcggt tgcccgaaaa
     2101 cggcatcatc ggccgcgatg gtaatgtgac aggtgagagc gagggatgcg ccggcaccca
     2161 ggcaccagcc gctcaccgcg gcgacgatcg gtttggaata gagccagcga ttgagttgcc
     2221 gctgcagaat atctttgcga tcggcgtctc tgaatcgatt gtatacttgg ttgagcgaag
     2281 ttccgtcagg gatcccgtag ggccatgcgg tcggcggatc gttatcttcg ctgatatctt
     2341 cgccgacaga gaaggccccg ttcgcgcccg tcaggatcag gccgcgaatc tcaggatcgt
     2401 tctcggcctc ggcaagcgcc tgatccagct cgctcatcag ttgttcgttc agcgcattca
     2461 gctctttggg ccgattgagc gtgataagca cgccgctgcg ctgcttttga taaagaatgt
     2521 ttttgtaatc aggcataaac tgctcctctc aattggccta atggagtctt gggcgattct
     2581 gaagacgtat cattacagca ctcgagctta tgatttcttt ttcgctctcg gtccaaacgg
     2641 ctcgggttga aacggaccgt cgcgcatttg caggtattcc cgaatacctt tgttggcgcg
     2701 tgcatcgtcc aagggcttgc gtagttcttc gcgcagcatc acgtgtgctg gcgcggacat
     2761 ttgcttgtca taggtcagcg cgtcgcggag tcccatcata tcgaggccca acgtggcgac
     2821 ctgcagattg atcttcacgg tttcgggcgg caccttcgcg atccgctcga caatcttaaa
     2881 gcactcatcg agcagttggt ccgcgggcac gactttgacg accaagccga tgcgcagggc
     2941 ttcattagcg tcgatgtgat caccggtgag accgtagcgc agcgcgttct tgtagccgcc
     3001 gagcaacgtc cacataaagc tcgtgttgga accgtggcgg acttcaggtt gggcgaatac
     3061 cgcgttctca gaggcgaggg tgatgtgggt atagagcgaa agccaggagc cggcgcccat
     3121 ggcccagcca ttgatcgcgc cgataatcgg cttgccgaac tcccacatgc gccgaaaatt
     3181 acgtggatcg atacgccacg aatcgatcac ttcggcggcg gtctgaccgt gcacaatgcc
     3241 gtaaggccag tgaatatcgc ggcgtctccc cgcgcgattg ccttgatcca tgcccgcgga
     3301 aaacgcgcgg cccgcgccgg tgagcacaat agcgcgaact tcattgtcct cttcgacttc
     3361 gtccaaggcc tgatgcaact ctttgatcat gctccggctg atggcgttca tcgcatctgg
     3421 ccggttaagc gtgatcagag cgccgccgcg ttttttttcg tagagaattt cctggtactc
     3481 gggcatgcag actcctttgg gttcacaggt tcaagtcgtc ggattatccg ataacggctt
     3541 gaacagtttg aacgattcga acgtttgaac tatcatttct tgttctttct ctcttcctcc
     3601 agctgcttca aaaacgacag atccatcacc tcggcgccct tgatgttcat ggtcgagggg
     3661 atttgtgagc acaaaatctc tcctaacctc cctttgttaa agggagggac agatgtcggc
     3721 cgataccgcc gttctctccg agcgaacgat tccgttgctg tcacttgatc tccactactg
     3781 catcgacggc aatgacgcct tgtcctttgg gtaagacaat caacggattg atgtccaagc
     3841 ccgcgatgct gtcgccgagg ctcattgcaa ggcgcgaaag gctgacgatc gcgtgacata
     3901 atgaatcgac atccgcggga ggtttgccac gcactccggc gagaatcgga aacccgcgca
     3961 gccgcgcgat catatcccgt gcttgctcga aagataacgg cggaatcccg acgacgacgt
     4021 ccttgagcaa ttcgacgaga atgccgccaa agccgcagag cactaccgga ccgaacaatg
     4081 ggtcgcgctt gatgccgaga atcatctcca cgcctacgcc ggtcatgcgc tgcacgatca
     4141 cgccggttat ttttgcgcta ggcgcgcgct gcgacacctc atcgtgcatg cgctcgtagg
     4201 cgacgcggac ttcgttggcg ttcgcgagtc ccaatgcaac gccgccgacg tcgcttttgt
     4261 gcgtaatatc gggcgattcg attttcaaca ccacgggaaa accgacgcgc tcggcggcga
     4321 gagccgcctc atcgacggaa cgggcaagca tcgtgggagc ggttggaatg ccgtattgct
     4381 gaaagagctg aaacgcggct tccgcaggca taatcccgct ggaaagtgtt ttgtcgagtt
     4441 gctcaacagt cggtgcggcc gtttggttac tgctgctttt tttgaactcg tagtattcgg
     4501 ctagtttttg aatgatggag gtggcgcact cggctccctc cataagcggc acgccggctg
     4561 ctctcaacgg caaaagaatt tccggatcca cgggcccacc aaccacggag ctgaaacagg
     4621 tgatcggctt tgtgctcgag ctggccgctt tttctatggc ggcggcaaag cggttgccgc
     4681 tcttgagctc tttcggccgc ggatcgttag cctcgagatt gatcgtgacc acgtctatat
     4741 tggagtcgtc gatcagcccc tgaagcagcc gcggaaatat gttggggtcg tcgtacatgg
     4801 cgccagtacc gtcgagcggg ttattcatgt tgccgaaatc cgctaccgct tgctgaagtt
     4861 gcaattttgt cggcttggtc aattctggaa tctccagacc gacattcgcc gccgcgtcgg
     4921 agacgagcgt gcattcgcca ccggaaccgc tcagcacgcc gatgcgcctg ccgccgccat
     4981 agagctttag gaatcgttcg caagaaaaga cactcaccgc gtcaatcaac tcgttcaaac
     5041 tacgcaccga tacaatgcca ccctgacgaa atgctgcatc gacgacttcc gccgcgccgg
     5101 caagagaacc ggagtgggta gcggccgcgc gtttagaaac ttccgatcgg ccgcccttga
     5161 ggatgatcaa gggctttttg tgttcgaccg cttttcgtgc aaccgcgagc aatgtttcgg
     5221 ctcgtttgaa gccctcgacg aaacccgcaa tgacgcgggt attttcgtcc tcaatgaaat
     5281 aattgaagta ttcctcgaat gtggcgccgg attgattgcc gcaggatacg atataactga
     5341 gaccgacgtg gcgattgagc atcaacgcgt tcgcgatggt cgtgctcagg ccgccgcttt
     5401 gagaaaccag cgcgaccggc ccggcaagaa acccaggagg aatcgcggaa gcaaataacg
     5461 gcgctttgcc aaacacattg agcacgccgt aacagttcgg gccgcagatg agaaagccgc
     5521 gctcgcgcga cagcgcttcg aggcgcgcct ggcgatgttt gccctcagca ccggtttccg
     5581 caaatccggc agcaaatacc acggcagcgc gtacaccggc gtctgcggcg gattccaaca
     5641 gatcgggtac cgagcgattg ggcaccgcca ggacaacgca gtctacaggt tcgggaatcg
     5701 ccgccagatc cggatagcag gggaaatcca tcacttcgcg atattttgga ttaatggcaa
     5761 tgattctgcc ggggtagccg aaatttttca agtttcggat caccctagtc ccgcgtggct
     5821 cgcggttcgg cagcgcgtcc gtccgctgcg atgccccgac gatagcaacc gagcgcgggt
     5881 tcatgagcgg cgagagatcg cgccagctgg tgtcgttatt ttgcgatt
//
LOCUS       DASULZ010000272         4705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_33996, whole genome shotgun sequence.
ACCESSION   DASULZ010000272 DASULZ010000000
VERSION     DASULZ010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33996"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VFY96_05685"
     CDS             <1..534
                     /locus_tag="VFY96_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5605986.1"
                     /translation="QLFIANLIGLSMLRELGPLMTAVILAGRSGSAFAAELGTMKVRE
                     EIDALKTMGLDPVRFLIVTRVIAAVFMTPLLTVFADLMGLIGGFVVMRSLGFPLVTYY
                     HQVQYAVTYGSLVGGLVKSFVFGILVAAIGCLRGLQTGTGATAVGQSTTSAVVSGIVL
                     ITITDGIFALVYYYLGV"
     gene            535..1338
                     /locus_tag="VFY96_05690"
     CDS             535..1338
                     /locus_tag="VFY96_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5605987.1"
                     /translation="MAEQPREKPIIQVQDFTAAYDGDVVLDKLNFEIYAGEVFAILGG
                     SGCGKSTLLKHMIGLYKPAGGRILIDGEDIAAVSGEERMKILRKFGVLYQSGALFGSM
                     SLLENVSLPLEEFTDLPPEAIRLIAMMRLQRVGLAHAADLMPAELSGGMQKRGAIARA
                     MALDPQIMFMDELSAGLDPVTSADLDQLVLNLSNNLKISFVIVTHELPSIYTIADRAI
                     MLDKQEKGIIAMGTPQELRDRNQDPRVQQFFHRQASNNHESSSVSANAR"
     gene            1341..2399
                     /locus_tag="VFY96_05695"
     CDS             1341..2399
                     /locus_tag="VFY96_05695"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5605988.1"
                     /translation="MERFSYFKIGVFVIAAVVIGVLGVVVLGAGALFQKKSIVETYID
                     ESVQGLDVGSPVKFRGVPVGRVEDITLTSVEYRTRLQYVLVRISISSNMFQFPVNDPS
                     SPVLQRELARGFRVRMAAQGLTGVAYLEADYLNPERNPPLRIDWKPDYPYIPSTRSRI
                     TQLSEAVERILRNVEEINIPQITASIEKSLAAMTKLAEGANLDKVGVQVNALLNELRD
                     TNRQVGALVSNPELKNAIADASATAGRARQIVERAEKPVNQFLADMPQATESLNRLVK
                     RLDAVANDLPQTSGELRQTIQRMNRLIANQQRDIEQTMGNLRAISENMKEITENSKKY
                     PSQTLFGGPPPPAKSMQR"
     gene            2396..3079
                     /locus_tag="VFY96_05700"
     CDS             2396..3079
                     /locus_tag="VFY96_05700"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEX5605989.1"
                     /translation="MNQSTRIITWIISFCLVCSGCVTLERSSPERHYFVIELPQSANS
                     PEAASDLVLSVGNMRISPRYADRSFVYRTSDTSYESDFYNQFLTSPDTMISEELRKGL
                     AASHQFKYVLGPADAQQPNYVLQGAVNALYGDFRKPSQPAAVLELEFFLYNDNSNNSG
                     LVAQKRYLKFVPVNERSPEALVKGWDQALQQIVAALAADLNSMNLNSKNSTPPVSPGK
                     DMSDAAESR"
     gene            complement(3024..>4705)
                     /locus_tag="VFY96_05705"
     CDS             complement(3024..>4705)
                     /locus_tag="VFY96_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5605990.1"
                     /translation="RLLTEAQLSSGGRADRFVAWDEATDRPAIYDPNTGQYERDGVMP
                     SLFGVRSLEGTDGNKITCKPVFERLGEIAVPFAPERSERIHRVPANKVWQTALLLAHN
                     RAVSMYMWNGLGQHTNATQTSRAIACLYALLGDFDRPGGNVIFPKVKVNEVDGKEFLP
                     KEAAAARIGRERKPLGPPAKPGNCAAYDVFAAIVEETPYPVKALLNFGSNTVMSTADS
                     RRGRDAMKKLEFAVAADLFMTPTAELCDYVLPATSFLEMSNMTTAFEHRQRGKTHLQY
                     RAAVVEPLAERRSDTWIIFELAKRLGLGDKFWNGDIEAAYNYELQPSGITLEELKANP
                     GGISRAIEPSYEKHASSPTHPPLTKHVLSEVEGGETGGFNSSRSHEQVTRGFATPTKR
                     VELYCKAFADHGISPMPEYIEPAISPLSRPDLAAQFPLVLTNAKFTTYVHSQQRALPT
                     LRKASPAPSADIHPETALRYGVKNKQWMTVESPRGAIRVKARVTTDIIPDVICCQHGW
                     WQDCKVLELPGYNPYEPTGANPATLIGTEIADPVSGSLPHRSYLCRVKPAE"
BASE COUNT         1007 a         1286 c         1353 g         1059 t
ORIGIN      
        1 cagttgttta ttgcgaactt gatcggactc tcgatgctgc gtgagctggg ccctttgatg
       61 acagcggtta tcttggccgg ccgctcggga tcggcttttg ctgcagagct gggtacgatg
      121 aaagtgcgcg aggagatcga tgcactcaag acgatggggc tcgatccggt gcggttttta
      181 atagtgacgc gagtgatcgc cgccgtattc atgacaccgc ttttgacggt gtttgccgat
      241 ttaatgggct tgatcggtgg cttcgtcgta atgcgctcgt tgggatttcc gctcgtcact
      301 tactatcatc aggtgcagta cgcggtcact tacggttcgt tggtgggagg gctggtgaaa
      361 tcgtttgtct ttggaattct cgtggcggcc attggttgct tgcgcggttt acaaaccggg
      421 actggcgcga cggcagtggg acagtcgacc acgagcgccg tggtcagcgg gattgttcta
      481 atcacaatca ccgacgggat ctttgcgctg gtttactatt atctgggagt ctagatggcc
      541 gagcagcccc gagaaaaacc gatcatccag gtgcaagact tcaccgccgc gtacgacggc
      601 gatgtggtgc tcgacaagct caacttcgaa atctacgcgg gcgaggtttt tgcaattctg
      661 ggcggctccg gctgcggtaa gagcactctt ttgaagcaca tgatcggact gtacaaaccc
      721 gccggcgggc gaattctgat cgacggcgaa gacatcgcag cagtatccgg cgaagagcgc
      781 atgaagattt tgcgcaaatt tggcgtgctc tatcaaagcg gcgcgttgtt cggctctatg
      841 agcttgctgg aaaacgtttc attgccgctg gaagaattca cggatttgcc gccggaagcg
      901 attcgcttga tcgcgatgat gaggctccaa agggtcggtc tcgcccacgc cgccgattta
      961 atgccagccg aactcagcgg cggaatgcag aaacgcggag ccatcgcgcg cgccatggcg
     1021 ctggatccgc aaatcatgtt catggacgaa ttatcggcgg gtttggatcc cgtaacttcc
     1081 gcggatctgg accaactcgt gctgaacctt tcgaataact taaaaattag ttttgtgatc
     1141 gtgacccatg agctgccgag catctacacg attgcggatc gtgctattat gttggataaa
     1201 caagagaagg gcataatcgc gatggggacg ccccaggagc tccgcgatcg caatcaggat
     1261 cctcgcgttc agcaattctt tcaccgtcaa gcttcgaaca accacgaatc gtcaagcgtg
     1321 agtgcaaacg cgcgttaagc atggaaagat tcagttattt taagatcggc gtctttgtga
     1381 tcgcggcggt cgtgatcggc gtgctcggcg tagtcgtcct cggggcggga gcgctgtttc
     1441 agaaaaaaag catcgtcgaa acttacattg atgagtccgt gcagggcctg gacgtcggct
     1501 cgccggtaaa attccgcggt gtgccagtgg gtcgagtgga agacatcacg cttaccagcg
     1561 tcgaatatcg aacccgattg cagtatgtgc tcgtgcgcat ttcgatttcc tccaatatgt
     1621 ttcagttccc ggtgaacgat ccatcgagcc cagtgcttca gcgagagctc gctcggggtt
     1681 ttcgcgttcg catggcggcg cagggattga ccggagttgc ctacttggaa gcggactacc
     1741 tcaatcccga gcgcaatccg ccgctacgca tcgattggaa acccgactac ccgtacatcc
     1801 cttccacccg aagccgcatt acccagctga gcgaagccgt ggagcgaatt ttgcgcaacg
     1861 tcgaagagat taacattccg caaatcaccg cgagcatcga gaagtcgctt gcggcgatga
     1921 ccaaattggc ggaaggcgcc aatttggata aggtcggagt tcaagtcaac gcactgctaa
     1981 acgagttgcg agacacgaat cggcaagtcg gcgcgctggt gagcaacccc gagctaaaaa
     2041 acgccatcgc cgatgcttcg gcaacggcgg ggcgtgcgcg tcagatcgtg gagcgcgcgg
     2101 aaaagccggt caatcaattt ctcgcggata tgccccaggc taccgaaagc ttgaatcgtc
     2161 tcgtgaaacg attggatgcc gtggctaacg atttgccgca aacaagcggc gaattgcgtc
     2221 agacgatcca gcgcatgaat cgattgatcg ctaaccagca gcgcgatatc gaacagacca
     2281 tgggcaatct gcgggcgatc tcggaaaaca tgaaagagat caccgagaac tccaaaaaat
     2341 atccctctca gactctcttc ggcggcccgc cgccgccggc caaatccatg cagcgatgaa
     2401 tcagtcgaca agaataatca cctggatcat ctccttttgt ctggtttgtt ccggctgcgt
     2461 gaccctcgag cgcagctctc cagagcgaca ttacttcgtg atcgaattgc cgcagagcgc
     2521 caattcgccg gaggccgcga gcgatctcgt cttgtcagtg ggtaatatgc gaatttcgcc
     2581 tcgctacgcc gacagaagct ttgtctatcg gacttcggac acaagctatg aatcggattt
     2641 ttacaatcag tttctcacgt cgcccgacac gatgatcagc gaggagctac ggaagggact
     2701 tgcggcctcg caccagttca aatatgtctt gggcccggcg gacgcgcaac agccgaatta
     2761 cgtcctgcaa ggagcggtga acgcgctcta cggtgatttt cgcaaaccaa gtcaacccgc
     2821 tgcggtgctg gagctcgagt tttttttgta caacgacaac tcgaacaatt cgggtctcgt
     2881 ggcacaaaaa cgttatctca aattcgtccc cgtgaacgaa agatcccccg aagcgctggt
     2941 caaaggctgg gatcaggcgc tgcaacaaat cgtcgcggcg ctggccgcgg atctcaactc
     3001 gatgaattta aattcgaaaa attctactcc gccggtttca cccggcaaag atatgagcga
     3061 tgcggcagag agccgctgac ggggtcggca atctctgtgc cgatgagcgt tgcgggattg
     3121 gcgcccgttg gctcgtacgg attatagccg ggtagctcca ataccttaca atcctgccac
     3181 catccgtgct gacagcaaat cacatcggga ataatatccg tggtcacccg cgctttgact
     3241 ctgattgcgc cgcgggggct ttcaacagtc atccattgct tgttcttgac gccgtagcgc
     3301 agcgcggttt ccggatgaat gtccgcgctc ggcgcggggg aggctttgcg cagcgtcggc
     3361 aaggcgcgct gttgactgtg cacgtacgtg gtaaatttcg cgttcgtcag caccagcgga
     3421 aattgagccg cgaggtccgg gcggctaagc gggcttattg cgggctcgat atattcaggc
     3481 atcggagaga ttccatgatc cgcaaatgct ttgcaataaa gttcgactct ttttgttggc
     3541 gtggcgaagc cgcgggtgac ctgctcatgg cttcggctcg aattaaatcc ccctgtttcc
     3601 cctccttcga cttcgctcag gacatgcttt gtcaaaggag gatgagttgg ggagctcgcg
     3661 tgcttctcgt aagacggttc tatggctcgt gatattccgc ccggattggc tttcaattct
     3721 tctagcgtaa tgccgcttgg ctgaagctcg tagttgtagg cggcctcgat atcgccgttc
     3781 cagaatttgt cgccgagccc caaccgtttc gccaactcga aaataatcca agtatcggag
     3841 cgccgctcag cgagcggttc aaccaccgcc gcacgatact gcaaatgcgt tttaccacgt
     3901 tggcggtgtt caaacgccgt cgtcatattc gacatttcca aaaagctggt cgcgggcaaa
     3961 acgtaatcgc aaagctccgc ggtcggggtc atgaataaat ccgccgcaac cgcaaactcc
     4021 agttttttca tggcgtcacg gccgcggcgc gagtcggcag tggacatcac ggtgttcgag
     4081 ccgaagttta aaagcgcttt taccggatac ggtgtttctt cgacgatagc ggcaaagacg
     4141 tcataggccg cgcagttgcc cggtttcgct ggtgggccga ggggtttgcg ctcccgtccg
     4201 atccgtgcgg cagccgcttc cttcggcaga aactccttgc cgtcgacctc gttcaccttc
     4261 actttaggaa agatcacatt gccgcccggc cggtcgaaat cgccgagcag ggcatagaga
     4321 cacgcgatgg cgcggctggt ctgcgtcgcg ttggtgtgct ggccgaggcc attccacata
     4381 tacatgctca cggcgcgatt gtgtgccagg agcagcgccg tctgccacac tttgtttgcc
     4441 ggcacgcggt gaatccgctc ggaccgctcc ggcgcaaacg ggacggcgat ttcgccaaga
     4501 cgttcaaaaa ccggcttgca ggtgatcttg ttgccgtcag ttccttcgag tgagcgcacg
     4561 ccgaaaagac tcggcataac accgtcacgc tcgtactgcc cggtgttggg atcgtaaatc
     4621 gctggccggt ccgtcgcttc atcccacgct acgaaccgat ccgctcggcc gccggacgaa
     4681 agttgagctt cggtgagcaa tcgat
//
LOCUS       DASULZ010000273         8176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_743233, whole genome shotgun sequence.
ACCESSION   DASULZ010000273 DASULZ010000000
VERSION     DASULZ010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8176
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743233"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..434)
                     /locus_tag="VFY96_05710"
     CDS             complement(60..434)
                     /locus_tag="VFY96_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605991.1"
                     /translation="MRSKAKILSALVLSSAMSLATYVVAQEKKGGDEKKSEEPAKAKA
                     EKVSGDIGLLDTEKHYLIVVTKDGKLITLDYNDKTKAQEVKETKVKMSDVGLGSSANV
                     EYVTKGDKNFLTKMEFRPAKGE"
     gene            complement(463..891)
                     /locus_tag="VFY96_05715"
     CDS             complement(463..891)
                     /locus_tag="VFY96_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605992.1"
                     /translation="MKSAVHLSGMAVISAALMFNGCSSHEEMAPKPAAPVPAPAAAPA
                     AKAEAKAPTEKSETKAKATKIAGDIGLLDTEKNYMIVVTKDGKLVTVDFDPKFQPSEL
                     KPEPAKISDIGLGSQAVVQYTKQGEKNVATSVEYKPAKGE"
     gene            complement(1798..3333)
                     /locus_tag="VFY96_05720"
     CDS             complement(1798..3333)
                     /locus_tag="VFY96_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5605993.1"
                     /translation="MDKKFWVLGIATSFLLAAGGARAQSVDDKIKALEQELTQLKEQQ
                     VELKKEATAAAAALPTFEYRPGNGLNVEAADKSWGVRFTIESHFRMLFESGRDQVGRT
                     NGEIMGRRFRPGILYCINNCLWEIEATLDLDGFGTGTGKNATGTDFGSILQRGAVNFH
                     AENLHPWLPTVQFGMEVQNAHGGSLARQGSGSVGAQAEYDLLTRNDGFNTGRAGQGIV
                     LNWDDRSLRGIGIPGRIGKFQLGMSSIGEGDDGLSSFTDRKDFNVYGNIQPFSQIKNK
                     WVSGLTFEYGSWFCNADDRALQNGCSRYRIQDHGDGGRQTLFDTGADTIGDGLTVAHG
                     PGIVWNVGPYTLRMMRSFEFSEDRGGTRGRKHSNNFLIGHDLFLWSPKGFLTGSSTTP
                     GSILIGTHFERANISVGCNGSTGITCASVVASGGSALLNQFHRIRVLLREWDLWYFIA
                     PRMSVGTSVLWYDSSNLRTGANQAAHNLGICDTPRVTPATGCRNGIGGDWVDVMLNWR
                     YTF"
     gene            3666..4427
                     /gene="hisF"
                     /locus_tag="VFY96_05725"
     CDS             3666..4427
                     /gene="hisF"
                     /locus_tag="VFY96_05725"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX5605994.1"
                     /translation="MLAKRIIPCLDVKAGRVVKGVKFVGLRDAGDPVEVARVYDRDGA
                     DELCFLDITASHENRGIILDVVARTAEQIFMPLTVGGGITTSEDIRNLLRAGADKVSI
                     NTAAVNRPEFVQEAAQRFGSQCIVVAIDAKRVGNHWEVFTHGGRNPTVLDACEWSKRM
                     ESYGAGEILLTSMDRDGTKAGYDLQLTRTISDAVDIPVIASGGVGELQHILDGVTEGG
                     ASAALAASIFHFGEFSIRDCKEFLAAHGVQVRPLR"
     gene            complement(4460..5665)
                     /locus_tag="VFY96_05730"
     CDS             complement(4460..5665)
                     /locus_tag="VFY96_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEX5605995.1"
                     /translation="MPIGTAFHSRTAPLCVSQNWRTWSGYFAASSYDVVHDYEYHAIR
                     NSAALIDVSPLYKYDIRGKDALRLVNRVMTRDAAKCSVGQALYGCLCQDDGAVIQDGT
                     VFRMTEDRFRFHLAEPSLRWLRLNATGMDLVIEEISEQIAALALQGPNALRILETLVD
                     VDLRALRFFRLTPATMAGLPVIISRTGYTGDLGYEIWLDVVYAGAVWDALIEKGKYFG
                     IKPVGMLALDVARLEAGFILLEVDYTSAEKAMIPSQRYSPFEIGLGWTVDLHKSDFVG
                     HRALRQERHPALSRQIVGLEISLTDYEYLFHQVGLPPQFPLAAWRGGVPVFKDDRQIG
                     HATTGAWSPILKKYIALATVNKPCAAPGTVLDIEVTVEHRRKTASAAVVKTPFFDPPR
                     KRAVGARLR"
     gene            5750..7348
                     /locus_tag="VFY96_05735"
     CDS             5750..7348
                     /locus_tag="VFY96_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5605996.1"
                     /translation="MTPMANYDAIVIGAGHNGLTAAAYLAKAGKKVLVLERRHVLGGA
                     AVTEEIYPGFKYSVCSYVVSLLRPEVIRELDLPKYDLEIIPLDSTFTPMPNGDYLFRS
                     GDHARTRREIARHSAADAEAYEEYGQLMIHMAMAVKPLLAMIPPEPTSWYPGDLFGLA
                     RLGNRFRRLGEKLFYDLAKLMTMSSADFLDQWFETDVLKATMSASGIIGTFMGPRSPG
                     TAYVLLHHYMGELDGAFRSWGFARGGMGSISRVIADAARRHGAEIRSQAPVDQVLIRR
                     GRACGVVLASGEEIHAKVIVSGIDPKRTFLKMIDAQCLSAEFKKQVSGFKIRGSSAKV
                     NLSLDSLPDFTSMPGPGPHLAGAISISPSIDYIEHAYDEAKYGAFSRRPYMDIILPSV
                     VDPSMAPPGKHVMSIFVQYAPYHLKTGSWTEKRDALGDTVVETLSEYAPNLKDIILHR
                     QVVTPWDLEEEFGLTEGNIFQGELTLDQLFFLRPVAGWAKYRTPIKNLYLCGACTHPG
                     GGVMAASGRLAALEILKDTRFRLL"
     gene            7354..>8176
                     /locus_tag="VFY96_05740"
     CDS             7354..>8176
                     /locus_tag="VFY96_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5605997.1"
                     /translation="MANYDAIIIGAGHNGLVTAAYLARTGKKILLLERRSIVGGIAVT
                     EEIFPLFKIPTCAHLVGSFSKKIISDLELDRHGLELLPLSPLLFAPAQEGSGLIIPRE
                     IPNAAQEIARHSKSDADRFQPFCELFKRLSEFLATLYQAPLPNKANPNHFHLAKFLEL
                     AWKFHRLGEKEMHEFLRILPMSIADLLNEWFESDLLKGALAGRAMLASFFGPRQQGSA
                     FNFLHHQLGESTGALPPAGFVRGGIGRLTEALNAAAKSYGAELRSAAEVTKIITAN"
BASE COUNT         1837 a         2300 c         2158 g         1881 t
ORIGIN      
        1 gcccggaaac gcagtgtcga accattagca ttagccgctc tatccagacc tattcactat
       61 tattcgccct tggcgggtct aaactccatt ttcgtcaaaa agttcttgtc ccctttggtg
      121 acgtattcca cgtttgcgga tgaccctaat cccacgtcag acattttgac tttggtttcc
      181 ttgacttctt gcgccttggt tttgtcatta tagtcaagag taattagttt tccgtccttt
      241 gtgacgacga tgaggtagtg cttctccgta tcgagcaagc caatatcacc ggagactttc
      301 tcagctttgg cctttgctgg ttcctccgac tttttttcgt cgccaccctt tttctcctgc
      361 gcgaccacgt atgttgccaa gctcattgcg ctcgacaaaa ctagtgcgct caaaattttg
      421 gcttttgatc tcattcggct ttcctccttc atctcggctt acctactcgc ctttggccgg
      481 cttatactcg acgcttgtgg ccacgttctt ctcaccctgc ttggtatact gcacgacagc
      541 ctgtgacccg agaccaatgt ccgatatttt cgctggttca ggcttcaact cgctgggctg
      601 aaatttagga tcgaagtcga ccgtcacgag cttgccatct ttggtaacga cgatcatgta
      661 gtttttttcc gtatccaaca gcccgatgtc acctgcaatc ttcgtcgcct tcgctttggt
      721 ctccgacttc tctgtggggg ccttggcttc ggccttcgct gccggcgcag ctgctggagc
      781 agggacgggg gccgcgggct taggcgccat ctcttcgtgg ctagaacagc cattaaacat
      841 tagtgccgca gaaattacgg ccatgccgga caagtgcact gcactcttca taattgcctc
      901 caatacgact aaagtcttta ggtacgacat gagtcgtata ctgtaaacgt ccgcggattg
      961 tcaaccatat cgaacggaaa ataatcaccc ctggctaatg ccactgacaa ttggatcgac
     1021 aacgatctac cccaaacctt gcaagacgcc atggaagacc gaacgagaac gcgagcggga
     1081 cgcgggaagg caatcgtgca tttacagttt tatgatctaa acaaagagat tggggaaagg
     1141 gctctggaca gactttcagg cagtcgtaat gtgtaatttg ctttctaacg ggctatgcct
     1201 gccaggtgca ttcgtccagc cttccgggcc aaagggcatg aaaatgatct tcgcagacta
     1261 agaagaaatg ggatgaaaat ggcgaaaatg agaagaaccc agctacgagt taaaacgata
     1321 aacgcgtcac cgacctgcga actcgcggat gagctcgcgg ctcgagatgt ttcagtgtcg
     1381 agtgatctga gcgttcatga tgtggagatg gttcggtgct agtaggcgtg cccgcaaagt
     1441 cagtctcagc agtatcccgc cgtgcaagcg cagcgtggag taacctgctg taatactgtt
     1501 gcggtacgag tcgacatccg aaacccatcg gtcaacgaaa gcatccggat aaacagcaac
     1561 cataaacgtc ggtgtgtgcc gctgcgaatt tgcattactg ttgtctattg ttcacggtct
     1621 gcgcataagc tgattagaca attaactcag cacggatttc agaggaagcc tgagcggcac
     1681 ccgagaagcg ggacaacgct aaaactttcg gctgggaaca gccccggacg tccgccaact
     1741 ataaaaaagg gaagccaaga gaggcttccc ttttttacgt aaaccctcta gatcaggtta
     1801 gaatgtgtat ctccagttga gcatcacgtc gacccaatcg ccgccgatgc cgttgcgaca
     1861 tcccgtagct ggagttacac gtggcgtgtc gcaaatgccg agattatgcg ccgcctgatt
     1921 ggctccagtg cggaggttcg atgagtcgta ccaaaggacg ctggtaccaa cgctcatgcg
     1981 cggtgctatg aagtaccaaa ggtcccactc gcgcagcaat acacggatcc tgtggaactg
     2041 gttgagcagc gctgagccac ctgaagcaac cacgcttgca cacgtgattc cggtggaacc
     2101 attacatcca accgatatgt tggcgcgctc aaagtgcgtg cctataagga tcgagccggg
     2161 ggtcgtcgac gatccggtca aaaatccttt ggggctccac aaaaacaggt cgtgaccgat
     2221 gagaaaattg ttactgtgct ttcggcctct cgtcccgccg cggtcttcgc taaattcaaa
     2281 agagcgcatc atgcgcagag tatacggtcc cacgttccag acaatcccag ggccatgcgc
     2341 cacagtcagg ccgtccccga tggtatccgc tccagtatcg aacaaagtct gccggccgcc
     2401 atctccatga tcctggatac gatatcgact gcaaccattc tgcagggcgc gatcatcggc
     2461 attacagaac caggagccgt attcgaacgt caaaccgctg acccatttgt tcttgatctg
     2521 ggagaacggc tggatgttgc cgtagacatt gaagtccttg cggtccgtga agctcgacag
     2581 accgtcgtcg ccttccccaa tactcgacat gcccaactga aacttgccga tccgcccggg
     2641 aataccaatt cctctaagcg agcggtcgtc ccagttcaac acgatgcctt gaccggctcg
     2701 gcccgtattg aaaccatcat tgcgggtgag gaggtcatac tccgcctgag cgccgaccga
     2761 tcccgagcct tggcgcgcca agctgccgcc atgcgcattt tgcacttcca tgccgaactg
     2821 aaccgtcggc agccagggat gcaaattctc agcgtggaag ttgaccgcgc cgcgctgcag
     2881 gatcgaaccg aagtcggtcc cagtggcatt ttttccggtg ccggttccga agccgtccaa
     2941 gtccagcgtg gcttcgattt cccagaggca gttattgatg caatacaaaa ttcccggcct
     3001 gaaccgccgg cccatgattt cgccgtttgt ccggccgacc tgatcgcggc cagattcgaa
     3061 cagcatgcgg aagtgtgatt cgatagtaaa ccgcacgccc caagatttgt ccgccgcttc
     3121 cacgttcaag ccgttgcctg gacgatactc gaacgtcggt agagcggcgg ctgccgctgt
     3181 agcctccttt ttgagctcca cctgctgctc ttttaactgc gtcagctctt gctcgagcgc
     3241 tttgatcttg tcatccacgc tctgagctcg tgcgccaccc gcagcgagaa ggaacgaagt
     3301 tgcaatgccc agcacccaaa actttttgtc cattcttgcc tccttttgcc tctacttttc
     3361 taaattacgt atttagtagt acttcgggcg ccttttttag cccattgatg gcgatcctgt
     3421 caagcctaaa gtggtactat ctaagtaaaa agatttaggc cagctcgtaa atcgatatag
     3481 ggcagcccac aaaaggggcg tcgtaaagcc acctcatggg caatattcat caatcgtctc
     3541 cgaaagacgc cgtaagcaac accttgactt tgttagcgtt tgaattgagg tgctcgtggc
     3601 tggagacatg taaccgcgct aggccgcttg tgaatctgct aggccaccgt tagattgacg
     3661 gacgcatgct cgctaaacgc attattccct gcctggacgt caaagctggg cgggtggtta
     3721 aaggtgtgaa gtttgtcggt cttagagatg ccggcgaccc ggtagaagtc gcacgcgtgt
     3781 acgaccggga tggcgctgac gagctttgct ttttagatat caccgcatcg cacgagaatc
     3841 gcggcataat tctggacgtg gtggcgcgca ccgccgagca gatttttatg ccgctcacgg
     3901 ttggcggcgg gatcaccacg agcgaggata ttcggaatct actcagggcg ggagcggaca
     3961 aagtttcgat caacaccgcg gcggtgaacc gcccggagtt cgttcaagaa gcagcgcaac
     4021 gattcggcag tcagtgcatc gttgtggcga tcgacgccaa gcgggttggc aaccactggg
     4081 aagtatttac ccatggcggc agaaatccca ccgttcttga tgcctgcgaa tggtcgaaaa
     4141 gaatggaaag ctatggggcc ggcgaaattc tcctcacaag catggaccgc gatggcacca
     4201 aagccggtta tgatctccaa ctgactcgaa cgatttcaga cgccgtcgat attccggtga
     4261 ttgcgtccgg cggagtaggc gaactccagc acattcttga cggggtgacc gagggcgggg
     4321 cgagcgccgc gctcgcagca tcgatctttc attttggcga gttcagcatc cgagactgca
     4381 aggaattttt ggccgcacac ggcgttcagg ttcgtccgtt gcgctaaacg cggatcaccg
     4441 gagtcttgcg ctatcactat catctcaaac gggcgccaac agcgcgtttc cgcggtggat
     4501 cgaaaaaagg cgtctttacg accgcagcac tggctgtctt tcgtcgatgc tcgacggtaa
     4561 cttcgatgtc gagcacggtt ccgggagcgg cgcacggttt gttcactgtt gcgagagcga
     4621 tgtatttttt taatatcggc gaccacgcgc cggtggttgc atggccgatc tggcggtcat
     4681 ccttgaagac tggcactccg ccacgccagg ccgctagcgg aaattgaggc ggtaagccaa
     4741 cctggtgaaa gaggtattca taatcagtga gactgatttc caatcctact atttggcgtg
     4801 agagcgccgg atgcctttcc tgtcgtaacg cacgatggcc gacaaaatca ctcttatgta
     4861 agtccaccgt ccaaccgagt ccgatctcaa atggagaata tcgctgtgat ggtatcatcg
     4921 ctttttcggc gctggtgtaa tcgacctcga gcagaatgaa tcctgcttcg agacgcgcca
     4981 cgtccagtgc cagcatcccc accggtttga tcccaaaata cttgcccttt tcaatgagcg
     5041 catcccaaac cgctccggcg taaaccacgt cgagccagat ttcatatccc aagtcgccgg
     5101 tataacccgt gcgggatatg atcacaggga ggcccgccat ggttgcaggg gtgagccgaa
     5161 aaaatcgaag agcacgcaaa tccacatcga cgagtgtctc caaaatccgc agagcattgg
     5221 ggccttggag cgccaaggcg gcgatctgct cggaaatctc ctcgatcact aaatccatac
     5281 cggtcgcgtt gagcctgagc cagcgcaggc tcggctcagc aagatgaaag cgaaagcggt
     5341 cctccgtcat ccgaaaaacc gtgccgtcct ggatcactgc cccatcgtcc tggcacagac
     5401 agccgtaaag ggcctgcccg acggaacatt tcgccgcatc ccgcgtcatc acgcgattga
     5461 ccagccgcag cgcgtccttc ccgcggatat cgtacttgta aagcggagag acatcgatca
     5521 aggcggcgga gttccgaatc gcgtggtact cgtagtcatg cacgacatcg taggagctgg
     5581 cagcaaaata acccgaccac gtgcgccagt tttggctgac gcaaagcggc gcagttcgag
     5641 agtgaaaggc tgtcccgatt ggcattcgag gattcgcgat gacaccgttc ggtgtgcaat
     5701 tccgcgttga cttcgcagac gaaattacca aatatgcgct cttaagcaaa ttacgcccat
     5761 ggcaaattac gacgcgatcg tcatcggcgc cggtcataac ggcctcaccg ccgccgcgta
     5821 tctagcaaaa gccggcaaaa aggttcttgt cctcgagcga cggcacgtcc tcggcggagc
     5881 cgccgtaacc gaagaaattt accccggctt taagtattca gtttgctcat atgtggttag
     5941 cctgcttcga ccggaggtta ttcgcgagct cgacttgccg aagtacgatc tcgaaatcat
     6001 tccgctcgat agcaccttca cgcccatgcc gaatggcgat tatctttttc gctccggcga
     6061 tcacgcgaga acacgccgcg agatcgcgcg ccattccgcc gccgatgctg aagcctacga
     6121 ggaatacggt cagttgatga tacatatggc aatggcggta aagcctctgc tcgcgatgat
     6181 ccctcctgag ccaacgagtt ggtatcccgg ggacctcttc ggacttgctc gcctgggaaa
     6241 tcgttttcgg cgtctgggcg agaagctctt ctacgatctc gccaaactca tgaccatgag
     6301 ctcggccgac tttctcgacc aatggttcga aaccgacgtc ctgaaagcga ccatgtccgc
     6361 gagcggcatc atcggtactt ttatgggtcc gcgttcgccc ggtacggcgt acgtcctgct
     6421 tcatcactat atgggcgaat tggacggcgc ttttcgctct tggggttttg cccgcggcgg
     6481 tatggggagt attagccggg tgatcgcgga tgcggcgcgc cgccacggcg ccgaaatcag
     6541 atcacaggcg ccggtggatc aggtgttgat caggcgtggc cgagcctgcg gggtagtgct
     6601 cgcatcgggc gaggagatcc acgccaaagt tatagtttct ggaatcgatc ccaaacgaac
     6661 tttcttgaaa atgatcgatg cacaatgtct ctctgcagag ttcaagaagc aggtgagtgg
     6721 gtttaaaatc cgcggctcct cggcgaaagt gaatctctcg ctcgattcgc ttcccgattt
     6781 cacttcaatg cccggtcctg ggccgcacct cgccggtgcg atctcgatca gtccgagcat
     6841 cgactatata gagcacgcct acgatgaggc caaatacggc gcattttcgc gccggcctta
     6901 catggatatc atcttgccat cagtagttga tccctcaatg gcgccgccgg gaaagcatgt
     6961 gatgtcgatt tttgtgcagt acgctcctta ccatctaaaa accggaagtt ggacagaaaa
     7021 acgtgacgcg ctgggcgata cggtagtcga gacgctcagt gagtatgcac caaacctgaa
     7081 agacatcatt cttcatcgac aggtggttac gccgtgggac ttggaagagg aattcggcct
     7141 cacggaagga aatatttttc aaggcgagct cactttggat cagctcttct tccttcgtcc
     7201 ggtcgccggt tgggcaaaat accgaactcc gattaaaaat ctttatctct gcggtgcctg
     7261 cacccatccg ggcggcggag tcatggcggc atcgggaagg ctcgcagctc tggaaatact
     7321 caaagacacg cgattcagat tgctgtaaca gccgtggcga actacgacgc gatcatcatt
     7381 ggcgccggcc acaatggcct cgtgaccgca gcttatctcg ctagaaccgg aaaaaaaatt
     7441 ctactgttgg aacgccgctc gattgtcggc ggcatagccg ttactgaaga gatttttcca
     7501 cttttcaaaa ttcctacttg cgcgcatctc gtcggaagtt tttcgaaaaa aattatctca
     7561 gacctcgagc ttgaccgcca tggcttagaa cttcttccgc tcagtcccct cctttttgcg
     7621 ccggcccagg agggaagcgg gcttatcatc ccgcgggaga tcccgaacgc cgcgcaggag
     7681 atcgccagac actcgaaatc cgacgcagac cgattccagc cattttgcga gctgtttaag
     7741 cgactttcgg aatttctcgc gaccctttat caagcaccgc tcccgaacaa agccaatccg
     7801 aatcatttcc acctcgccaa atttctggaa ctcgcttgga agtttcatcg acttggcgaa
     7861 aaagagatgc acgagtttct ccggatcttg cccatgagca tcgccgacct attgaacgag
     7921 tggttcgaaa gcgacttgct caagggcgcc ttggctggac gcgctatgct tgcttccttt
     7981 tttggcccgc gccagcaagg gagcgccttc aatttccttc atcaccaact tggcgagtcc
     8041 actggcgcgc ttccgccggc aggtttcgtc cgcggcggca taggccgttt gaccgaagct
     8101 cttaacgctg ccgcaaaaag ctatggggcc gaactccgca gcgcggcgga agttacaaag
     8161 attatcaccg ccaacg
//
LOCUS       DASULZ010000274         2151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1453532, whole genome shotgun sequence.
ACCESSION   DASULZ010000274 DASULZ010000000
VERSION     DASULZ010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453532"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VFY96_05745"
     CDS             complement(<1..480)
                     /locus_tag="VFY96_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5605998.1"
                     /translation="MNKISFGVRVPNSGPLSTIENIVKATKSAEEMGFDAIWVHDHVV
                     WSSEMHRHHISSGAFEAITDTQEANFFEATTTLSYLAAETKKIVLGVACLVMPCRNPV
                     YAAKQYSTLDHLAQGRLLVGVGLGSKATRESDEFGVFGVPYERRGDRTDEYIEAMKAI
                     "
     gene            complement(647..1567)
                     /locus_tag="VFY96_05750"
     CDS             complement(647..1567)
                     /locus_tag="VFY96_05750"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5605999.1"
                     /translation="MRTEKINFYSEGIKLAGVLYLPDSEQAKPYPGIVQGPGFLGLKD
                     AKHYIMMFEKLCAVGYACLCFDYRGWGDSEGEQRGWVMPKWQAEDIRSALSYLETRPD
                     IDADRLATYGSGGTGGGNAVYVAATDSRVKCCVSYLGVSSGREWLHSMRREYEWIDYL
                     KSIEADRKQRALTGKGAIVSAREGGIMVQTPERQTTTIKKDVADKIPDAMPLACAEAI
                     LEYSPIDVVHKISPRGVLFIAVEGDAVTPEQESFDLYEKAGEPKKLVLYRKTTHYGIY
                     NDYFDDVAANVIDWYDRHLKYHRVEVTEKH"
     gene            complement(1700..>2151)
                     /locus_tag="VFY96_05755"
     CDS             complement(1700..>2151)
                     /locus_tag="VFY96_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5606000.1"
                     /translation="TPASARRAAEYCDGWMPGRIPMLTFIKMVDYLREQYKAQGRPMG
                     TIGAIPIVSIDKDLDTALSKVNTPGLIKEGNNPSKKTWVNSGNFKSYEDIRGLLLAGK
                     PEDVAKDTKEYEKNGLEHIVYDLRFRYADWYQQIDYLGKEVLPAVRA"
BASE COUNT          451 a          616 c          559 g          525 t
ORIGIN      
        1 aatcgccttc atcgcctcga tgtattcatc cgtgcgatca ccgcgtcgtt cgtacggaac
       61 gccaaaaacg ccgaactcgt cggactcgcg cgtcgccttc gagcccaagc caacgccgac
      121 gagcaaacgt ccctgcgcga gatgatcgag ggtggaatac tgcttggcgg catagaccgg
      181 attgcggcag ggcatgacca agcacgcgac gccgagaacg atctttttgg tctctgcggc
      241 gaggtatgac agggtcgtgg tggcttcgaa aaaattggcc tcttgcgtat cggtaatcgc
      301 ttcgaatgcg ccggaagaga tgtgatgtcg atgcatttcg gagctccaga ccacatgatc
      361 gtgcacccag atggcgtcga atcccatctc ttcggccgac ttcgttgcct tgacgatgtt
      421 ttcgatcgtc gatagcggac cggaattcgg cactctcact ccgaaggaaa ttttgttcat
      481 gattggctcc tcataccttg aattttcttg acgtcattcc ggccaagctg gcgatacgct
      541 agcgcgagcc agaatccagg agaagaggag gggcctagat tcctgctttc gcgggaatga
      601 tgacgggacg aacagcgaat tttcaaccct ctgggcaaag cccgcattaa tgtttttcgg
      661 ttacttccac cctatgatac ttcaagtgac gatcgtacca gtcgatcaca ttggctgcga
      721 catcgtcgaa ataatcgttg taaatcccgt aatgcgtggt ctttctgtat aacaccaact
      781 tcttcggctc gcccgccttc tcatacaaat cgaaagattc ctgctccggc gtcaccgcat
      841 ccccttcgac agcgatgaac agcacgccgc ggggcgagat cttgtgcacg acatcgatgg
      901 gactgtattc caaaattgct tcggcacaag cgagcggcat ggcatcgggg attttgtccg
      961 cgacgtcttt tttaatcgtc gttgtctggc gctccggagt ttgcaccatg ataccgcctt
     1021 cccgcgcgct gacgatggcg ccttttccag tcaatgcgcg ctgcttacgg tccgcttcaa
     1081 tgctcttgag gtaatcgatc cactcgtatt cgcgtctcat cgaatgaagc cactcccgcc
     1141 cgctgctgac accgagataa ctgacgcagc atttgacgcg agagtcggtg gccgcgacgt
     1201 aaaccgcatt gccgccgccc gtcccgccgg agccataggt ggcgagtcga tctgcatcga
     1261 tgtccggccg cgtttccagg tagctcagcg cgctccgaat gtcttctgcc tgccatttcg
     1321 gcatcaccca accgcgctgt tcgccttcgc tgtcacccca accgcggtag tcaaagcaca
     1381 gacaagcgta gccaacggcg cagagctttt cgaacatcat gatgtaatgt ttagcgtcct
     1441 tgaggccgag aaagcccggg ccttgaacaa tgccgggata cggttttgcc tgctccgagt
     1501 cgggcagata cagcacgcct gctaacttga tgccttcgct ataaaaatta attttttcgg
     1561 tacgcatacg aactccagta gggttcaatc acttcattcc gttcaatcgc tccgctcagt
     1621 tcaaatcgtt caagccgtcg gacccggatt ctccgaaacg gtttgaacag cttgaaccgt
     1681 tggaacggtt ggaacaattt tacgcacgca ccgcgggcag cacttctttg cccaggtaat
     1741 cgatttgctg gtaccaatcg gcatatcgga aacgcagatc gtaaacgatg tgctcgagac
     1801 cgtttttttc gtattccttc gtgtccttgg cgacgtcttc cggcttgccc gccaagagca
     1861 agccgcgaat atcttcgtag gatttaaaat taccggaatt aacccatgtt tttttactgg
     1921 gattgttgcc ttctttgatc aatccaggcg tattgacttt gctgagcgcc gtatcgaggt
     1981 ctttgtcgat gctgacgatg ggaatggcgc cgatcgtccc cataggtcgc ccttgggcct
     2041 tgtactgctc gcgcaggtag tcgaccattt tgataaaggt cagcatggga atccgtccag
     2101 gcatccagcc gtcgcaatac tcggccgcgc ggcgagccga agccggcgtt c
//
LOCUS       DASULZ010000275         2520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_236239, whole genome shotgun sequence.
ACCESSION   DASULZ010000275 DASULZ010000000
VERSION     DASULZ010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236239"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1310
                     /gene="acnA"
                     /locus_tag="VFY96_05760"
     CDS             <1..1310
                     /gene="acnA"
                     /locus_tag="VFY96_05760"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEX5606001.1"
                     /translation="KPKPWVKTSLAPGSRVVTEYLKDSGLLSALENIGFHLVGYGCTT
                     CIGNSGPLPEPVANAIQAGDLVAAAVLSGNRNFEGRIHANVRANYLASPPLVVAYALA
                     GSMDVDLYHDPLGRDSQGKPVYLKDIWPSPEEIHETMRKSVRSEMFKREYSHATEGDD
                     RWNKMPVPQGELFKWDMQSTYVRQAPYFEGMTKTSGEPKPIHGARVLALLGDSVTTDH
                     ISPAGSIEKNGPAARYLTNNDVLPKDYNQYGARRGNHEVMVRGTFANIRLKNFLAPGT
                     EGGVTVHLPDKKQMSIYDAAMQYQKEGVPLIVIAGKEYGTGSSRDWAAKGPRLLGIKA
                     AIAESFERIHRSNLIGMGIVPLVFKPGENLKTHNLTGFERFDILGIGAGLKLHQELTV
                     KASADDGKVTEFKVICRIDTPAEMDYYRHGGILEYVLRQLLAS"
     gene            1362..2273
                     /locus_tag="VFY96_05765"
     CDS             1362..2273
                     /locus_tag="VFY96_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5606002.1"
                     /translation="MTRREALATLGKSSLALLFAARRRAEGMAAEGKIMATPDFIGKF
                     TANNPGIMTLQGTNQYVVGRENVVVIDVALSADSNLDGIIEQVEAMGAKKIDKILLTH
                     IHSDHCGGALALRKRCGAKLGIHRSRKGYLGGEDFQYDDNDRISFGDGELNVLHTPGH
                     ESGHCCFYESDERILFSGDNILGYGTAVIHPPDGNMTDYLKSLERLLGFDISLILPGH
                     GPLVGKPEAKIREYIKHRLEREQQVIAALHQGRETIGDVTQTIYVDVSPALRRVAEFS
                     VQAHLEKLMREGRVKQEGTHYRLVSEN"
BASE COUNT          594 a          677 c          722 g          527 t
ORIGIN      
        1 tcaagccgaa gccatgggtg aaaaccagcc tcgcgcccgg gtcgcgggtt gtgacggaat
       61 acttgaagga cagtggtttg ttatctgctc tggaaaatat cggctttcat ctggtcggct
      121 atggttgcac gacttgtatc ggcaatagcg gtccgctccc cgagccggtc gcgaacgcca
      181 ttcaagcggg cgatctcgtc gcagccgcgg tgctgtccgg caaccggaac ttcgaaggcc
      241 ggattcatgc gaatgtgcgc gccaactatc tcgcttcacc gccgctagtg gtcgcctatg
      301 cattggcagg gagcatggat gtcgatcttt accatgatcc gctgggccgg gattctcaag
      361 gcaagccggt ttatttgaaa gatatctggc cctcgccgga agagatccat gagacgatgc
      421 gcaaatccgt gcgctcggag atgtttaaga gagaatacag ccatgccacg gaaggcgacg
      481 atcgttggaa caagatgccg gtccctcagg gggagctctt caaatgggat atgcagtcca
      541 cctacgtgcg acaggcgccc tattttgagg gcatgactaa aacttcgggc gagccgaaac
      601 ccattcacgg cgcccgcgtg cttgcactgc tgggcgattc cgtaacgacg gatcatatct
      661 cccctgcagg atcgattgag aagaacggac cggcggcgcg ttatctcacc aacaatgacg
      721 tcttgcccaa ggattacaat caatacggtg cgcgtcgtgg caaccatgaa gtgatggtgc
      781 gcggaacgtt cgcgaatatc cggttgaaaa atttccttgc gcccgggacg gagggcggcg
      841 tcacggtgca cttgcccgac aagaagcaga tgtcgatcta cgatgccgcc atgcagtatc
      901 aaaaagaggg cgtaccgctg attgtcatcg ccggcaagga gtacggcacc ggatcatcgc
      961 gcgattgggc ggccaagggg ccgaggctcc ttggtatcaa agcggccatc gccgaaagtt
     1021 tcgagcgtat tcatcgcagc aatctgatcg gcatgggaat cgtaccgctg gttttcaagc
     1081 cgggcgaaaa tctcaaaacc cataatctga ccggcttcga aaggttcgat atcttgggca
     1141 tcggtgcagg gctaaagctg caccaggaac tgaccgtcaa agccagcgcc gatgacggca
     1201 aagtgactga gttcaaggtg atttgccgca tcgatacgcc ggcggaaatg gattattacc
     1261 gccacggcgg tattttggaa tacgtcctgc gccagttact tgcaagttga actgaagtag
     1321 ggcgtaaaat tttacgccac tacgggttgc tgaaataagt gatgacacga cgcgaagcgc
     1381 tggcgacgct cggcaaaagc tcgctggcgc ttttgtttgc tgcgagacgc cgcgccgagg
     1441 gaatggccgc tgaagggaag attatggcga caccagactt cattggaaag ttcacggcaa
     1501 acaatccggg catcatgacg ctccagggta ccaatcaata cgtggtgggc agagagaacg
     1561 ttgttgtgat cgatgtggcc ttgagcgctg attccaacct cgatggaatc atcgagcaag
     1621 tcgaagccat gggagcgaag aaaatcgaca agattttgct cacgcacatt cacagcgatc
     1681 attgcggcgg cgcattggcg ctgcgcaaac gttgcggcgc caagctgggc attcaccggt
     1741 cgcgcaaagg ttatctcggc ggcgaggatt ttcagtatga cgacaatgat cggatctcat
     1801 tcggcgatgg cgagctcaat gtgctacaca ctccgggcca tgaatcgggc cattgctgtt
     1861 tctatgagag cgatgaaagg atcctattca gcggcgacaa cattctcggc tatggcacgg
     1921 cggtcattca tccgcccgac ggcaacatga ccgactatct caaatctctc gagcggctgc
     1981 tcggcttcga catcagcctg atcctgccgg gtcacggtcc cctggtcggc aaacctgaag
     2041 ccaaaattcg agaatacatc aagcatcgac tcgaacgaga gcaacaggtc attgcggcgc
     2101 ttcaccaagg acgcgagacc atcggcgatg tcactcaaac gatttatgtc gacgtttcac
     2161 ccgcgttacg gcgagtggcg gagttctccg tccaagcaca tctggaaaag cttatgcgtg
     2221 aaggtcgcgt aaagcaggaa ggaacgcact atcgcctggt cagcgaaaat tgacggggag
     2281 cgccctgata aaactggaac tatcatcacc tcagcttcga tcgccagagc ctgccacgcc
     2341 tatacaatcc attgcgtagt tgatccgaag aagttctcac catagccgaa acgaccgtcg
     2401 cgtgcttgca gaagcgaagc acggttcccg gtgcttattg gcttcgcctt cattcaccta
     2461 tttctccatg atgatgaccg agtaacccgc ttggctgatt tgcctggccc gttgctctgc
//
LOCUS       DASULZ010000276         4102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_440001, whole genome shotgun sequence.
ACCESSION   DASULZ010000276 DASULZ010000000
VERSION     DASULZ010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440001"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..507)
                     /locus_tag="VFY96_05770"
     CDS             complement(151..507)
                     /locus_tag="VFY96_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606003.1"
                     /translation="MGSLLDVLASPGIAFLPYLKMGVALTDVQASVLDACSGDTCGVR
                     SVRARNDSTKPSWAVGGGLEYALSQNWIVKGEYLFIGLGLHDRLRVSGPGLGTVGGST
                     WVWSQDLSSVHTGKMV"
     gene            complement(1061..1136)
                     /locus_tag="VFY96_05775"
     tRNA            complement(1061..1136)
                     /locus_tag="VFY96_05775"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1101..1103),aa:Thr,seq:cgt)
     gene            complement(1137..1442)
                     /locus_tag="VFY96_05780"
     CDS             complement(1137..1442)
                     /locus_tag="VFY96_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606004.1"
                     /translation="MGETLEKIATYLQPNPVLSLGVALVAGFAAVKTVAYDQRAGFIL
                     FALVGVVGLFLGEFVLLYYGLDGYLESVSEFRILFDLIAAYIGSFIVAAIIHFIKPT"
     gene            complement(1446..2072)
                     /gene="thiE"
                     /locus_tag="VFY96_05785"
     CDS             complement(1446..2072)
                     /gene="thiE"
                     /locus_tag="VFY96_05785"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX5606005.1"
                     /translation="MPTPNFDLYLVTDRLHTAGRDLLWVLEQALEGGVKAIQLREKDL
                     SGKELFILAEKTRRLCQRYQAALFVNDRLDVALAIDAAGVQLGKPSLPLADARTLLGN
                     SRFIGASVHSLEEAQSAERGGADFLVFGPVYFTPSKAAFGAPQGVPALKKIVEIARIP
                     VYAIGGITPDNITELRSACVERVALISAITEASQPKQAAEEIIRLLRR"
     gene            complement(2082..2855)
                     /locus_tag="VFY96_05790"
     CDS             complement(2082..2855)
                     /locus_tag="VFY96_05790"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX5606006.1"
                     /translation="MEDFFEIAGNKYRSRLIVGTGKYKDFAETKRAIEVSGAEIVTVA
                     VRRVNIIDPNKENLLDYLDPKKYTILPNTAGCYNAEDAVRTCRLAREAGVGKLVKLEV
                     IGDDKTLFPDVPATIEAAKILVKEGFVVLPYINDDPITAKRLEEIGCAAVMPLAAPIG
                     SGLGIRNPYNIRIILEQAKVPVIVDAGVGTASDAATAMEMGCHGVLMNTAIASAKDPI
                     LMAEAMRLGIEAGRKAYLAGRIPKKLYATASSPLDGLLG"
     gene            complement(2858..3058)
                     /gene="thiS"
                     /locus_tag="VFY96_05795"
     CDS             complement(2858..3058)
                     /gene="thiS"
                     /locus_tag="VFY96_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEX5606007.1"
                     /translation="MKLKINGEEREVADGLTVTALLEEFKIRPARVVIERNREIVARE
                     AYDITHLADGDTLEIVHFVGGG"
     regulatory      complement(3144..3255)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3378..>4102)
                     /gene="amrB"
                     /locus_tag="VFY96_05800"
     CDS             complement(3378..>4102)
                     /gene="amrB"
                     /locus_tag="VFY96_05800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEX5606008.1"
                     /translation="TEKNRSIIAGLVPHAGPVYSGPCAAHLFSQLDTPPQRIIILGVN
                     HRGQGHRAALSPWPRWQTPLGEIAVNQHLNDFIRSRVNFLAEERSAHSEEHSIEIELP
                     FLQYTLTDFEFVPISLSRLSLDECSDLGAAIAAACQAEANTHTLVLASSDLSHYLLPD
                     RTEALDRLALEPILALDPKGLLRVIGQENISMCGVIPTAIMLFAANAMGAQRARLLKH
                     CHSGDVMPMHKVVGYAAVAIEH"
BASE COUNT         1003 a         1159 c         1087 g          853 t
ORIGIN      
        1 cgtagctgcc acgctggcgg cgacaaccga gttagctaac acgcctaagt tcggcgccgg
       61 ctcgccacct agacgagaag gggcgaaaca tcagcttaat aacttcactc aaatctctcg
      121 acagataaag tgcttactaa aacttgtaac tcacaccatt ttaccggtgt gaacgctgct
      181 taaatcttgg ctccacaccc acgtgcttcc tcccacggtg cccaaccccg gccccgagac
      241 gcgcaagcga tcatgaagtc ctagcccaat gaacaaatat tcgcccttaa caatccaatt
      301 ctgactgaga gcatactcga gaccaccacc gacagcccag gatggcttgg tcgagtcatt
      361 acgtgctctg accgatctca ccccgcaggt atctccgcta catgcatcca gcacggacgc
      421 ttgtacgtcg gtgagagcca cgcccatctt gagatacggg agaaaagcga tcccaggcga
      481 cgccaagacg tccagcaggg accccatacc aagctcctac tcttgtccga gagaccgggt
      541 cggatgccga gctaagcgga tcagttttgc taccgcgtaa actgaggtat ccaaattcac
      601 cctcaaaacc gaaaacggta gaagcaacct ggaaattgta gccagcgata ccagcgcggg
      661 tgaagcggcc atccgagtca tatcgccagg agtgcccagc gccgttgtaa cttgtacccg
      721 gcgcaaatac tcccccggct gaaccttgct ctgtggtttt cacatcagag aaaccagcgc
      781 cgccgagatg tatgcctaca taagcaccgc tccagtcaac cgaggtagcg ggtgtggcta
      841 aaaccgagcc cgctaacatg agaatcgttg cagttataag agcagcgtta cagtcgcacc
      901 tcagaagaga gcctacgggt gctaactaag gtcgatccgc ctgagggaaa aggcatctaa
      961 aaagagtgct tagcgacgaa cagggacaga acacggacaa aatgcagccg tttagaccaa
     1021 atgattttta acggacaggc ttctaagtct tcaaaaagac tggagccacc cggcggattc
     1081 gaaccgccga cctactgatt acgaatcagt tgctctaccg actgagctag ggtggctcag
     1141 gtgggtttga tgaaatgtat gatcgccgcg actatgaacg agccaatgta cgcggcgatt
     1201 aaatcaaata agattctgaa ttcagaaaca gactctaaat acccgtcgag cccgtagtaa
     1261 agtaacacga actcgccgag gaataaacca accactccaa cgagcgcaaa gagaataaag
     1321 ccggctcgct gatcgtaggc gacggttttc actgccgcga atcctgcgac caaggcgacg
     1381 cccagcgata acaccggatt aggttgtaaa tacgttgcga ttttctcaag tgtttcgccc
     1441 aaaccttacc tccgaagcag tcggataatc tcttctgcgg cttgctttgg ttgggaggct
     1501 tcagtaatcg cggaaatcag cgcgactcgc tcgacgcagg cgctccgcag ttcagtgatg
     1561 ttatccggcg tgatgccgcc gatagcataa acagggattc gcgcgatttc cacaattttc
     1621 ttgagggccg gaacgccttg gggcgcgcca aacgccgctt tggaaggggt gaaataaacc
     1681 gggccgaata caaggaagtc cgcgccgcca cgttccgcgc tttgcgcttc ctcgagggag
     1741 tgaaccgaag caccgataaa gcgactgttg ccaagcagtg tgcgcgcgtc agccagtggc
     1801 agcgacggtt ttcccagctg cacaccggcg gcatcgatcg ctaaagcgac atcgaggcgg
     1861 tcgttaacaa acaacgcagc ttggtagcgc tggcagagtc ggcgcgtttt ttcagcgagg
     1921 ataaacagtt ccttaccgct caagtccttt tcgcgtagct gaatcgcttt aaccccgccc
     1981 tcgagcgcct gctccagcac ccagagtaga tccctaccag cggtgtgcaa tcggtcggtt
     2041 acaagataga gatcaaaatt cggtgttggc aattgcgcgg ttcaacctaa gagtccatcc
     2101 agcgggcttg aagcggtggc ataaagtttt ttgggaattc ttccggcaag gtaagctttg
     2161 cgtcctgctt cgataccgag acgcatcgct tcggccatca aaatcggatc tttggcactg
     2221 gcaatggcgg tattcatcag tacgccgtga caacccattt ccatagccgt cgcggcatcc
     2281 gaagcggtgc cgaccccggc atcaacgatc accggcacct tcgcttgctc taagataatt
     2341 cgaatattgt atggattgcg aataccaagt ccggagccaa tcggcgctgc caacggcatc
     2401 accgccgcgc agccaatctc ttccaaacgc ttggccgtga tcggatcgtc attgatatac
     2461 ggcagcacca caaagccttc cttcaccagg attttggccg cctcgatggt ggccgggaca
     2521 tccggaaaga gagttttatc atcgccgata acctcgagct taaccaattt tcccacccct
     2581 gcttcccgcg ctaagcggca ggtgcgaacg gcgtcttcgg cgttataaca gcctgccgta
     2641 ttgggcaaga tggtgtattt tttcgggtcg agatagtcga gtaaattttc cttgttggga
     2701 tcgataatat tgactcgccg cactgcgacc gtaacgattt ccgcgccgga aacctcgatg
     2761 gcgcgcttgg tctccgcgaa gtctttgtat tttcccgtgc cgacaatcaa tcgggagcga
     2821 tatttatttc ccgcaatttc aaaaaaatct tccataacta tcctccgccg acaaaatgga
     2881 cgatctcaag ggtatcaccg tcagcaaggt gcgtaatgtc gtaggcttca cgggcgacaa
     2941 tctcccgatt gcgttcaatg acgacgcgcg cgggccgaat tttaaactcc tcgaggagcg
     3001 cggtgacagt aagtccgtcg gcgacttctc tttcctcgcc gttgattttc aatttcataa
     3061 caagaaaaaa accgcatccg cggcagcctc gcagagttgc cagggaatac ggtttcaagt
     3121 ttgtcccgct ctacaaagcc gagacggcga ctctcacgct tccctacgcc ggtgcgaccc
     3181 ggatcaggtt caaagggtta cctgaattga tcaggctctc agtctgcctg cgggcagaca
     3241 cccctagcat cgaaagcacc cttaacaaag cagtatcggc aagtcaataa actcacgagc
     3301 tgccgttcaa attgttccag tggttcacac gggttgaatc ggcgagcccg agacaaatct
     3361 tccggatgac tcaggtctca atgctcgatc gccacggctg cgtagccgac cactttatgc
     3421 atcggcatca catcgccgga atggcaatgc tttagtaatc gcgcgcgctg agctcccatg
     3481 gcattcgctg caaacagcat gatcgcagtg ggtataacgc cgcacatcga gatgttctcc
     3541 tgtccaataa cacgcagcag acctttggga tcgagcgcca aaatgggctc gagggccaag
     3601 cggtcgagcg cctcggtccg gtcgggcaag agataatggc tgagatcgct gctggccaaa
     3661 acgagcgtgt gggtattggc ttcggcttgg catgctgccg caatggcggc gcccaggtcg
     3721 ctgcactcgt cgagagacaa acgcgacagc gatatgggga caaactcgaa atcagtcagg
     3781 gtgtattgca ggaacgggag ctcaatttca atgctatgct cctcagaatg ggcactccgc
     3841 tcctcggcga ggaaattcac gcgcgagcgg atgaaatcgt tcaggtgctg attgacagcg
     3901 atctcgccga gcggggtctg ccaacgcggc cacggactca gcgcagcgcg atgaccctgc
     3961 ccgcggtgat tgacgccgag aataatgatc cgctggggtg gagtgtcgag ttgactgaaa
     4021 aggtgcgcgg cgcacggccc ggaataaacc ggacccgcat gaggaacgag gccggcaatg
     4081 atggagcggt ttttttccgt gg
//
LOCUS       DASULZ010000277         4238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_337734, whole genome shotgun sequence.
ACCESSION   DASULZ010000277 DASULZ010000000
VERSION     DASULZ010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337734"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VFY96_05805"
     CDS             complement(<1..440)
                     /locus_tag="VFY96_05805"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606009.1"
                     /translation="MDVLNITGYQRRTPHVVADCKGFFAKEDLELNFHRTTFAPDHNR
                     GMAEGRWDFTLSSADTMIARTTTDGVDYVLFMQAEEGLSAYLIGQPEVRSIEQLRGQL
                     LAGDPGDSNLDLIRKKILRMHGIDESDYQIEIIGSSPVRLEAFL"
     gene            561..1298
                     /locus_tag="VFY96_05810"
     CDS             561..1298
                     /locus_tag="VFY96_05810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5606010.1"
                     /translation="MELENKVALITGGSRGIGKAVAAAFAREGAKLALCARSADELQQ
                     TVAELRGMKAQAEGWMCDVTVEASVKEFVHQVQKTYGRIDILVNNAGVMTRPVPMVEL
                     EVRKWDYTIAVNLRGPFLVTQAVLPFMLKQKSGSIINVSSTIGRAAYPNFVAYATSKW
                     GLEGFSQTLAAELNSSQIRVNSVEPGYVATKLTGYHGSKPESITDVFVYLASDESQAV
                     TGKMLSSSTWKSQTKLNANFDHLRSTD"
     gene            complement(1295..1474)
                     /locus_tag="VFY96_05815"
                     /pseudo
     CDS             complement(1295..1474)
                     /locus_tag="VFY96_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
     gene            1769..3619
                     /locus_tag="VFY96_05820"
     CDS             1769..3619
                     /locus_tag="VFY96_05820"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5606011.1"
                     /translation="MMPRQFPSQSRPLLLALLISFTPLLTLSGWSFQDGHQHLTITEQ
                     LRRDVQFLTSPELTGRGVDTPGIKLARDYLAADFARAGLRPGGDDGSFFQRFDVAVGV
                     TVDQPSSLTLDQNAPLDLNTDWIPLGLSKSDNVDGGLVFAGYGITAKENGYDDYTDVN
                     VKGKIVLILRYEPPPKDEKSPFKKPPEYSVHAALRTKANNARDHGAVGMILVDLNHDN
                     EEQPELLSTKNSLWRGGNSLVAAQVRRGVLEKWLNSRGVSLSGLKEKIDRTGKPGSMA
                     LPGALARLQVTLKENHDRAENVIGILPGADSNLRDQRVVIGAHYDHLGFGHFGALDRQ
                     AEGKVHPGADDNASGTAVLLELARRLAKLPVKPERTIVFTAFSGEELGLYGSRHYIEH
                     TDSISAINAMINLDMVGRLRENRVTVFGARSGENLSDLVARNARGLGLDITDSNDVGR
                     SDHMSFYSKKIPVLHFFTGTHGDYHRATDTWEKLNIEGMAKISDLVLVTALEIANSKD
                     PIRFVSLPSRSPGSIGTARGVGVYLGTIPDYGAVATGVRLAGVSSGSPAAEAGLREGD
                     VIVRLDDKDIQNIEDLTDALQAHKSGDQVSVVVLRAGATITLRATLSSRG"
     gene            3743..>4238
                     /locus_tag="VFY96_05825"
     CDS             3743..>4238
                     /locus_tag="VFY96_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX5606012.1"
                     /translation="MIKLYTFPPSTNSRKVRIALLEKGLEFERINIDLTKREQKNPEY
                     LKIHPFGQVPALDDEGFIIYDSTVINEYLEDEYPYPALMPKDSEGRARARLLEDFRDT
                     RFNPYFVHLMQEIRKPEGERDSQRIDSARGEIIKSFDRIEKELNGKEYLVGSFSLADI
                     AFMSN"
BASE COUNT          972 a         1150 c         1166 g          950 t
ORIGIN      
        1 tcgagaaatg cctctaatcg cactggcgag ctgccgataa tttcgatttg ataatcgctc
       61 tcgtcgattc cgtgcatgcg aagaattttt ttccggatca ggtcgagatt ggaatcgcct
      121 ggatcacctg ccagaagttg tccgcgtaat tgctcgatcg agcgcacttc cggctgcccg
      181 atcaagtacg cgctcagccc ctcttccgcc tgcatgaaca atacgtagtc gacaccgtcc
      241 gtggtggttc gcgcgatcat ggtgtcggcg ctgctcagag taaagtccca gcgtccctcc
      301 gccatgccgc gattgtgatc gggcgcgaag gtagtccgat gaaaattgag ttcaagatct
      361 tctttggcga aaaaaccttt gcagtccgcc accacgtgcg gcgttctgcg ctgatagccg
      421 gtgatgttca gtacgtccat aaacattctc ctgcactctt ttatcttcag ccaagaaatg
      481 cctcaagagc tccgtgttgg cttcgatcac caatacagct atcaatcaat agagaaacga
      541 agtttacggg gcagattttt atggagctcg aaaataaggt tgcgttgatt accggcggca
      601 gcagaggcat cggcaaagcg gtcgcggcgg cgtttgcgcg cgaaggagca aagctagcgc
      661 tctgcgctcg tagcgccgat gagcttcagc aaaccgtagc tgagcttcgc ggcatgaaag
      721 cgcaagcaga gggctggatg tgcgacgtca cagtggaagc gtcggttaag gaattcgtgc
      781 accaggtaca aaagacgtat ggtcggatcg atatcctggt caacaacgcc ggggtcatga
      841 cgcggcccgt accgatggtc gaactggagg ttaggaagtg ggactacaca atcgccgtga
      901 acctccgtgg cccatttctg gtgacgcaag ccgtgttgcc gttcatgctc aaacaaaaaa
      961 gcggctcgat tatcaacgtt tcgtcgacga taggccgcgc cgcctatccg aactttgtcg
     1021 cctatgccac gtcgaaatgg ggcttggaag gatttagcca gactctcgct gcagagctga
     1081 attcgagcca gatccgggtg aattccgtgg agccgggcta cgtcgctacc aagctgacgg
     1141 gttatcacgg cagcaagccc gaatccatca ccgacgtgtt tgtctatttg gcgtcggatg
     1201 aatcccaagc cgtaacgggc aaaatgctga gctcctcaac ctggaaatca caaaccaaat
     1261 taaacgccaa ctttgatcat ctgcgatcaa ccgattaaag cccgagcaat tcgatgagct
     1321 ttgcctggtc gaagccgact acggattgcc cgtcgatgat cgttaccggc gtgctctgaa
     1381 agccttgatc gagcaattct ttcagcgccg ccttgtcggc gcgaacattt ttttccacga
     1441 actgaacgcc cttttgagat agaaactcca cctctttgtg acacggccct cagccgggtt
     1501 gggtgtaaac aacaactgat ttagccattg gtcctcctcg gcttcttaga atcaattgta
     1561 taacagttta atgcggaaaa gtcgcccagc cagtgtggtg accggcaact cgcatcacga
     1621 atgacatgtg ccagcctgca acgagcacaa ttctgcttct ttcgcgcgag aagcatcctg
     1681 tggctcgacc gaattttcga gcacaaacca aacagaattc ctagcgagct tcaacagcaa
     1741 acgaaaacca tgctagtcta ctaatcgcat gatgccgcgc caatttccgt cccagtcccg
     1801 tccgctactt ctagcgcttc tcatcagctt cacgcctctc ctcactcttt cgggttggag
     1861 ttttcaggat gggcatcagc atcttacgat tacggagcaa cttcgacgcg acgtacagtt
     1921 tttaacctcg cccgagctta cggggcgagg cgtcgatacg cccggcataa agctcgcgcg
     1981 agattatctc gccgccgact ttgcacgcgc tggattgcgg cccgggggcg atgatggcag
     2041 cttcttccag cggttcgacg tcgcggtcgg ggtcacagtt gaccagccga gcagccttac
     2101 gctggatcag aatgcgccac tggacctcaa cacggattgg attccgctcg ggctttcaaa
     2161 gtccgacaat gtcgacggcg gactggtctt tgccggctat ggcattaccg ccaaagagaa
     2221 tggttacgat gactacaccg acgtgaacgt caaaggcaaa attgttctga tcctgcgtta
     2281 cgagccgccg ccaaaggacg agaagagtcc ttttaagaaa ccgcccgagt actcggtcca
     2341 cgccgcactc cgcaccaaag cgaacaatgc tcgcgatcac ggcgccgtgg gcatgattct
     2401 ggtcgacttg aatcacgaca atgaggagca accggagttg ctttcgacga aaaatagctt
     2461 gtggcgcggc ggcaacagtc ttgtcgcggc acaggtcagg cgcggagttt tggaaaaatg
     2521 gttgaattcg cgcggcgtct ctcttagcgg actcaaagag aaaatcgatc gcacggggaa
     2581 acccggctct atggcgcttc ccggcgcgct cgccaggttg caggtcactc ttaaggagaa
     2641 tcatgaccgc gcggaaaatg taatcggcat cctgcccggc gcagattcca atctgagaga
     2701 tcagcgggtg gtcatcggtg ctcattacga tcatcttggg ttcgggcact tcggagcgct
     2761 cgaccgccaa gcggaaggca aagttcatcc tggtgccgac gacaatgcct cgggcacggc
     2821 cgtgctcctc gagctcgcgc gtcgccttgc gaaattgccc gtgaaacctg agcgcacgat
     2881 tgttttcacc gccttttcag gggaagagct cggactttac ggctcgcgcc attacatcga
     2941 gcacactgat tcaatttccg cgatcaacgc catgatcaat ctggatatgg tcggccgttt
     3001 acgggagaac cgggtcacgg tctttggcgc ccggtcgggc gaaaacctga gcgatctggt
     3061 agcccgaaac gctcgcgggc ttgggctgga catcactgat tccaacgacg ttggccgcag
     3121 cgatcacatg tcattctata gtaagaaaat tcccgtgctt catttcttca ccggcaccca
     3181 tggagactat catcgtgcca cggatacgtg ggagaaactg aatatcgaag gaatggcgaa
     3241 gatcagcgat ctcgttctgg tgaccgcgct cgagattgcc aatagcaaag atcccatccg
     3301 gtttgtaagt ttaccttcgc gctctcctgg aagcattggc actgcgcgcg gagttggcgt
     3361 ctatctcggc acgattcccg attatggggc ggtggccact ggcgttcggc tggcgggcgt
     3421 ctcgagcggt agtccggcag cggaagctgg actgcgtgaa ggcgacgtca tcgtccgact
     3481 tgacgacaaa gacattcaaa atatcgaaga tcttaccgat gccctgcaag cgcataagtc
     3541 gggcgaccaa gtgagcgtcg tggtgctacg cgccggcgcg acgattacgt tacgggcaac
     3601 gctgagcagc cgtggttaac ctgcgtgttc aggtatgcaa ctctctgctt aactgcgcga
     3661 aaattcaaaa aattgctcgc aggcggcggt attcttatgc taatatgcgc tcaagtggtg
     3721 ccggcaagga ggtcagctgt cgatgatcaa gttgtatacg tttccgccct caacgaactc
     3781 gcgcaaggtg cgcattgcgc tcctggagaa ggggcttgag ttcgagcgga tcaatatcga
     3841 tctcactaag cgggaacaga aaaatcctga gtacttgaag attcatccgt ttggtcaagt
     3901 gcctgcactg gacgacgagg gttttatcat ttacgactcc accgttatca acgagtatct
     3961 cgaggacgag tatccgtacc cggcgctgat gcccaaagat tcggaaggtc gcgcgcgagc
     4021 gcgcctgtta gaggattttc gggatactcg tttcaatccc tattttgttc atttgatgca
     4081 ggagatacgc aagcccgaag gcgagcgcga ttcccagcgc atcgatagcg cgagagggga
     4141 gatcatcaag tcatttgatc ggatcgaaaa ggagttaaac ggcaaggaat acttggtggg
     4201 ctccttcagc ctcgccgata tcgcttttat gtcgaata
//
LOCUS       DASULZ010000278         3882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1150871, whole genome shotgun sequence.
ACCESSION   DASULZ010000278 DASULZ010000000
VERSION     DASULZ010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150871"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..911
                     /locus_tag="VFY96_05830"
     CDS             99..911
                     /locus_tag="VFY96_05830"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5606013.1"
                     /translation="MFASIRHIAVMTENWDREAKFYQTIFGMKKITNGMTDEKGEYNK
                     NRGHLSDGVIGLALLQRQPGFRSGLDHFGFAVDDVQLVRDRLKQHYPEVFVAQSQSHV
                     PFAGLRSHDPDGNQFDLSQKGMANVREGYVEFGWEQPRWINHICIRSGRPAHIAEYYQ
                     KVFDLRPVEGLSGDNSYYLSDGKVSLAIRPWDMVQYRGHMAGLDHFGYKVDDLEQTKK
                     DLEALASATPASAPRTIAIGRDGETRQKNLEGCSLCKYALADPDGVLLDLTD"
     gene            1063..1416
                     /locus_tag="VFY96_05835"
     CDS             1063..1416
                     /locus_tag="VFY96_05835"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5606014.1"
                     /translation="MDFADINDTIRMANAENKRFVPLFSDENFRSWILYFRPGEQTDM
                     HYHLSPETFLVLQGRASVKGIKGDERVIEKNEIVFFGAKDYYQITNVGTEPLVLVGNR
                     SEAFGGAHVTANDAS"
     gene            1458..2444
                     /locus_tag="VFY96_05840"
     CDS             1458..2444
                     /locus_tag="VFY96_05840"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606015.1"
                     /translation="MPALHPRWIIFLFVFLCTSFPEISPSASLEPIKIASTGPGISTL
                     PLEIAALKGFFRAEGLDVLTITMRANIAVNALLTRNVEFATPSTSIIKTATAGLPVKL
                     IAVLLGRPDYFLTVRKEIKSVRDLKGKRIAIGTFGAAADLALRAVAKDEGLDAERDLI
                     RLQMGGAASRYASLLAGSVDATILTLPYNLEAEKAGYRDLLWFGERMELPLSGLAVRD
                     ESLRNNPKQVLAVVRAIFRAMAFAYGNREETTQIISRWLKVSPEIAARSYDLGKSSWS
                     SGGVVSDAAVRVLVDQSIAELKIKSSIPLDQVRNWSFAEQVRRELANAAPAR"
     gene            2464..3579
                     /locus_tag="VFY96_05845"
     CDS             2464..3579
                     /locus_tag="VFY96_05845"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX5606016.1"
                     /translation="MTLSLEQMVESYDPGKSLEQASTPPAYWYRDPRILELEYRTVFS
                     RAWQAIGRSDQLREPGHYITSELATEPVLAVRGSDGCLRGFYNVCRHHAAIVMTQSEG
                     QTETLRCPYHGWTYDLQGALVLAPEFGGVAKFDRSAISLVPIEIDVYKNGVFAKLEHG
                     NPSLQDFLGKDLVERFEALNLEQLKWFERRSYTLNCNWKVFVDNYLDGGYHVPHLHRR
                     LNSVLDYGHYTIETGKRFCLQSSPIVPDSSGGDAGELRRGERALYYWIYPNLMINIYA
                     GVMDTNLVVSRGVDRTEVIFDYYFADVSAEAKKNNLASVTLSEQIQAEDVAICESVQR
                     GLNSRSYSAGRLSLRREAGEHLFHKLLYTDLKAGLSG"
     gene            complement(3598..>3882)
                     /locus_tag="VFY96_05850"
     CDS             complement(3598..>3882)
                     /locus_tag="VFY96_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX5606017.1"
                     /translation="GWWKNFRYIYLPLLRPGLMAGWFWVMVHAYRELTISLMLARSSN
                     RTASVIIYDLWSNGSFQQLSAFGVIMFSILIVLVAIAQKISAHYGIQERI"
BASE COUNT          896 a         1063 c         1037 g          886 t
ORIGIN      
        1 atcgggaccc gagaaccgaa acgtgagtcg agcgcttcgg cggttgacag caagcaccaa
       61 ctctgagatg aatccgacac aagaggagga taataaccat gtttgcaagt atccggcaca
      121 tcgccgtcat gaccgagaac tgggatcgcg aggcaaagtt ttatcagact attttcggca
      181 tgaaaaagat caccaacggc atgaccgacg agaaaggtga atacaacaag aaccgcggcc
      241 acttgagcga cggcgtgatc ggcctggcgt tgctccagcg ccagcccggc ttccgctccg
      301 gactcgatca ctttggtttt gccgtcgacg atgtgcaact ggttcgcgat cgcttgaagc
      361 aacactatcc ggaggttttt gtcgcacaga gccaaagcca cgttcctttc gccggactca
      421 ggagtcatga tccggacggc aatcaattcg atctttccca gaaaggcatg gccaacgtgc
      481 gtgaaggtta tgtggaattc ggctgggagc agccgcgttg gatcaatcat atctgcattc
      541 gcagcggccg gccggcgcac attgccgagt attatcagaa agtttttgat ctgaggcccg
      601 tggagggact gtccggcgat aacagctact atcttagcga tggcaaggtg agcctcgcaa
      661 tccggccttg ggatatggtg cagtatcggg ggcacatggc cggtctcgat cattttggct
      721 acaaagtaga cgatctggag cagacgaaaa aagatctcga agccctcgcc agtgccacac
      781 cggcttcggc gccgagaaca atagccattg ggcgcgacgg cgaaacccgt cagaaaaatc
      841 ttgaaggttg cagcctctgt aaatatgcgc tggccgaccc cgacggcgta ctgctggatc
      901 tgacggacta atctttgtgt gagcggattg cgtcgcaatt gtcatttcga gctcctttga
      961 ctttgctcag aatagactcc ggcgagaaaa tctttcttca tattgtagct gacaacctat
     1021 gggccatccg cgcatcgcgg tatagtctgg aggaggaaac gtatggactt cgccgatatc
     1081 aatgacacga tccgcatggc gaacgcggaa aataaacgct ttgtcccgct cttcagtgac
     1141 gaaaactttc gctcctggat actttacttt cggcccggcg agcagacgga catgcattat
     1201 catctttcgc cggaaacctt tctggtgcta cagggcagag cttcggtcaa agggatcaaa
     1261 ggcgatgagc gggtgattga gaagaacgaa atcgtttttt tcggcgccaa ggattattac
     1321 cagatcacca atgtcggaac tgaaccgctg gttctggtcg ggaaccgctc agaagccttc
     1381 ggtggtgcgc acgtcaccgc aaacgatgcc tcataggtat ttgccagctt tactgctcgt
     1441 ctgatcacgc ggtcgtcatg cccgccttgc atccgcgctg gattattttc ctgttcgtgt
     1501 tcctctgcac atcgtttcca gagatatctc catcagcttc cctcgagccg ataaaaatcg
     1561 cttccaccgg tcctggaatc agcacgctgc ccctcgagat cgcagcgctc aaaggttttt
     1621 ttcgtgctga agggcttgat gtgctcacca tcactatgcg cgcgaatatc gccgtcaacg
     1681 cccttttgac tcgcaatgtt gaatttgcca cgcctagcac atcgattatc aaaaccgcca
     1741 ccgcaggtct tccggtcaag ttgatcgccg tcttactcgg ccgtcccgac tactttctta
     1801 cggttcgaaa agaaatcaaa tccgtaagag acttgaaagg taaacgcatc gccattggga
     1861 ctttcggggc tgccgccgat ttggcactac gcgcagtcgc gaaagatgaa ggattggacg
     1921 cggaacgtga cctaatccga ttacagatgg gcggagccgc ttcccgctac gcctccctgc
     1981 tcgcgggtag cgtggatgcg accatattaa ctctccctta caaccttgag gcggagaaag
     2041 ccggctaccg agatctcctg tggtttggtg agcgcatgga attacctttg tccgggcttg
     2101 cggtgcgcga tgagagcttg cgcaacaatc ccaaacaagt acttgcagtg gtgcgcgcca
     2161 tcttccgcgc catggcgttt gcttatggca accgcgagga aactacgcaa attatttcgc
     2221 gctggctcaa agtctctccg gagatcgcgg ccagaagtta cgacctggga aaaagcagtt
     2281 ggtcatccgg cggagttgtt tccgatgccg cggttcgtgt tttagtcgat cagtccatcg
     2341 ccgaattaaa aatcaagtcc tccatccctc tcgatcaagt ccgcaactgg agttttgccg
     2401 agcaagtccg gcgggaactg gctaacgccg cgcccgcacg atgaggctct gagccgatca
     2461 cgcatgacgc tttcgcttga gcagatggtc gaaagttatg atcccggcaa gtcgctggag
     2521 caagcatcga ctccgccagc ttactggtat cgcgatcctc ggattctcga actcgaatat
     2581 cgcacagtgt tctcgcgggc ttggcaggca atcggccgct ccgaccagct ccgtgaaccc
     2641 ggtcattaca tcacatccga actggccact gagcccgtgc tcgccgtcag agggagcgac
     2701 ggttgtttgc gcggctttta taacgtctgc cgccatcacg cagcgattgt gatgacccaa
     2761 tcagaaggac aaacggagac cttgcgttgt ccttatcacg gctggaccta cgatcttcaa
     2821 ggcgcgcttg ttctggctcc ggagttcggc ggggtcgcta aattcgaccg ttccgcgatt
     2881 agtttggttc cgatcgaaat cgatgtgtac aaaaacgggg ttttcgcaaa gctagagcac
     2941 ggaaatccgt cgctccaaga ttttttgggc aaagatctcg ttgaacgctt cgaagcgttg
     3001 aatcttgaac agttaaagtg gttcgagcgg cgcagctata ccctcaactg caactggaaa
     3061 gtttttgtcg acaattacct tgacggcggc tatcacgtcc cgcatcttca ccgcaggtta
     3121 aacagcgttc tcgattacgg ccattatacg atcgagacgg gcaagcgttt ctgtctgcaa
     3181 tcgagcccga ttgttccaga ttcctccggc ggcgacgccg gcgagctgcg ccgaggcgag
     3241 cgggcgctct attattggat ctatccgaat ctcatgatca acatttacgc cggcgtgatg
     3301 gataccaacc tcgtggtttc gcgtggcgtt gaccgcacgg aagtcatctt cgattattat
     3361 ttcgccgacg tctcggctga agccaaaaaa aacaatctcg ccagcgtgac cctcagcgag
     3421 cagatccaag ccgaagacgt ggcgatctgc gaatcggtgc agcgcgggct caactcgcgt
     3481 tcctactcgg cggggcggct ttcgctccgg cgcgaagcgg gggaacacct ttttcacaaa
     3541 ctgctatata ccgatttgaa agcgggtctt agcggctgat cttgcaccgg aatcgcttta
     3601 gattcgctcc tgaatcccgt aatgcgcgct gattttctgc gcaatcgcaa ccagcacaat
     3661 cagaattgaa aacataatca caccgaaggc gcttagctgc tgaaaggagc cattgctcca
     3721 gagatcataa atgatcacag aagcggtgcg attgctcgag cgcgccagca ttaatgaaat
     3781 cgtcagttcg cggtaggcgt gcaccatcac ccaaaaccat cctgccatta gaccggggcg
     3841 taagagtggc aagtagatat agcgaaaatt tttccaccaa cc
//
LOCUS       DASULZ010000279         5683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1048283, whole genome shotgun sequence.
ACCESSION   DASULZ010000279 DASULZ010000000
VERSION     DASULZ010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048283"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VFY96_05855"
     CDS             <1..814
                     /locus_tag="VFY96_05855"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606018.1"
                     /translation="GYNDGKNIVFDYRSAGGKPERIPAFIAELLQLKADLLFCPNLPC
                     INAAMDATKTIPIVMVSNVDPVQLGIVKSFARPGGNITGLTLQSLELSGKRLELLKEL
                     FPKLKRVGLVWNLGDHSMNLITDQIQAAAPPLGVTLQPFGVREPEDFGGIFDKMSQNP
                     PDAVFSIADRLITSQRNQILDFGVKSKIPTMFDAAPAVEAGALMAYGPSRADVARRAA
                     FYVEKILKGAKPADLPVEQPTKFEFVLNLKTANQIGLTIPQTVLMRADRVIR"
     gene            910..1938
                     /locus_tag="VFY96_05860"
     CDS             910..1938
                     /locus_tag="VFY96_05860"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606019.1"
                     /translation="MKLRNSHPASSQSPKTRVIGCAICSLLFLLSGSADAQQPPRIVR
                     LGYLSSSRPTSDTERSEGIKLALGQLGYREGENIVYHFRYAEERLDRLPQLAAELVRL
                     HVDVIIAAGGGPVIQAPINATQTIPIIMAGQGADPVAAGFVKSLSKPGGNVTGMTNLL
                     VQLGDKRLELLKEAVPKITRVAVPYVPENATHSLELKEVQSAARALGITVQTFEIRHR
                     HDFDKAFTSIDKYHPDGLQLLGGPVIRESQKRIIDFALNKRLPSVFTSVEAVKGGGLL
                     YYGADLSETYRRVAFYIDKILKGTKPADLPVEQPTKFELVINLKTAKEIGLTIPPNVL
                     ARADRVIK"
     gene            2248..3030
                     /locus_tag="VFY96_05865"
     CDS             2248..3030
                     /locus_tag="VFY96_05865"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5606020.1"
                     /translation="MFSAAAEYERFMGRWSRRLAPEFIAFAGIKNGDRVLDVGTGTGS
                     LASALERTMPFSEIVGIDPSEGFISYAKKNAKSNRTHFEIGDAQALRFQDGSFDKTMA
                     LLVMNFIPNHDRAIGEMRRVTRPQGGVSACVWDYDEGMQMLRFFWDEAVALNPAIEPK
                     DERHMKLSHQGELGELWKKAGLVSIQEKPLSIDQAYDSFDDYWGSFLKGAGPGGAYVV
                     SLPDKSRQELATRLRKRLLGNRENGPFVLKARAWCVRGEVPK"
     gene            3457..4452
                     /locus_tag="VFY96_05870"
     CDS             3457..4452
                     /locus_tag="VFY96_05870"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606021.1"
                     /translation="MRQNWLSTKLIGCIYLLPFLFMVSLQPALAQQPKKTPRVGILRV
                     GSPPDVFIDTLRQGLHDAGYIEGQNIFLEYRWLKREDQFAEAAEDLVRLNVDSIVVTS
                     TVGALAAQRATRSIPIIVPVMGDPVGSGLVTSLAHPGGNLTGFSNLAPELWPKRLELF
                     KELVPKLSRVAMLWNVNNPAMATGAKGTREAASAMNITIQDRGARDPNELDSVFTALE
                     KQRPDGVLVMIDAFTLRNSKKIIDSIANARLPAMYDEKSPIAAGGLVSYGPDFVDLFR
                     RTAIYVNKILKGAKPADLPVEQPIKFELVINLNAAHQIGLTVPSNILARADQVIK"
     gene            4462..5193
                     /locus_tag="VFY96_05875"
     CDS             4462..5193
                     /locus_tag="VFY96_05875"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606022.1"
                     /translation="MRQQAQGISKKFQAFALFAFGLSLLFGAVIARAQIPKNISRVGI
                     LFVGGRDQPHLAAFKNALSERGYTEGKNIVFEYRYGEGRYDRLTDLAEELVRDHVDVI
                     VTTSSVSAVAVRKATRTIPIVMTSGNPLEQGLAASLAQPGGNVTGLSLFVGDLSGKRV
                     ELLKEAFPDLTRVACLWSPREDPSTTAFKETERAAKAISIQLHSIELRNAADIDKGFA
                     VLPRSVKAVFGNHESACDTPFEGHR"
     gene            5153..5464
                     /locus_tag="VFY96_05880"
     CDS             5153..5464
                     /locus_tag="VFY96_05880"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEX5606023.1"
                     /translation="MNPLVTLHSKVIVELALKHRLVGMYPTRQFAEEGGLLAYGPLIS
                     DLYRRAAIYVDKILNGAKPADLPIEQPTKFEFLANLKTARQIGVTIPPNVLARADQVI
                     K"
BASE COUNT         1400 a         1523 c         1498 g         1262 t
ORIGIN      
        1 cggatacaat gatgggaaaa atatcgtctt tgactaccgc agcgcaggcg gaaaaccaga
       61 gcgcatcccc gcctttatcg ccgaacttct gcaactgaag gcagatcttc ttttctgccc
      121 aaacttaccg tgcatcaatg ctgccatgga cgccacgaaa acaatcccca tcgtaatggt
      181 ttcaaatgtc gatccagttc agcttggtat cgtaaagagt ttcgcgcggc ccggtggcaa
      241 tatcacaggc ttaaccttgc agtccctaga attgagcgga aaaagactcg agctacttaa
      301 agaacttttt ccgaagctta aacgtgtggg gctcgtgtgg aatttaggcg atcacagcat
      361 gaacctgatc accgaccaaa ttcaagccgc cgcgccaccc ttgggcgtca ccctccagcc
      421 ctttggtgtt cgagagcccg aagattttgg cggcattttc gataagatgt cgcaaaaccc
      481 gcccgacgca gtgttttcga ttgcagacag actgatcact tctcagagaa accaaattct
      541 agacttcggc gttaaaagca agattcccac aatgttcgat gccgcaccgg ctgtggaggc
      601 tggcgcattg atggcttatg gaccgagccg agccgacgtt gcacgtcgag cggcctttta
      661 cgtggagaaa attctcaagg gcgccaagcc tgccgatctt cccgtcgagc aaccgaccaa
      721 attcgagttt gtccttaatt taaagaccgc gaatcagatc ggcttgacga ttccgcaaac
      781 tgtcctcatg cgagcggata gagtaattcg ctgaaggcgt gagctgtaag acgagtcaaa
      841 atcaaaaact aaactcgtaa atcacaggaa ttcgacccaa caaactgcga caagctgatc
      901 gggtactcgg tgaaactacg caatagccat ccggcaagca gtcaaagtcc gaaaacgaga
      961 gtcatcggtt gcgctatttg ctcgctgcta tttttgctgt ccggttctgc tgacgcgcag
     1021 cagccaccgc gaatagttcg tctcgggtat ctctccagct ctcggccaac gagcgatacc
     1081 gagcgttcgg agggcattaa gctcgccctg ggtcagctcg gctacagaga aggcgaaaac
     1141 atcgtctatc attttcgcta tgcggaggag aggcttgatc gactcccgca gcttgctgcg
     1201 gagctcgttc gactgcacgt cgatgtcatc attgcggctg gaggaggacc ggtcatccag
     1261 gcgccgatca atgcgactca aaccattcca attattatgg cgggccaagg cgccgatcct
     1321 gtggcggcag gcttcgtgaa gagtctctct aagccgggcg gcaatgtcac cggaatgacc
     1381 aatctgctcg tgcaactcgg cgacaaacgc ctggaactcc tgaaggaagc tgtgccaaaa
     1441 attactcgtg tggctgttcc ttacgttccg gaaaatgcga cccattcact tgaactcaaa
     1501 gaagtgcaga gcgcggctcg agcgctgggc ataacggttc agacattcga aatccgacac
     1561 aggcacgatt tcgacaaggc ttttacatcc atagataaat accatcccga tggactgcaa
     1621 cttctcggcg gcccagtgat ccgcgaaagt caaaaacgga taatagattt cgccctgaac
     1681 aagcgtctcc cgtccgtgtt cacttccgta gaagccgtga agggcggcgg gcttttatac
     1741 tacggcgcag acctcagtga gacctaccgg cgcgtagcct tttatatcga caaaattcta
     1801 aaagggacca agccggctga tctccccgta gagcagccga caaaattcga gttggtcata
     1861 aatctaaaaa ccgccaaaga gatcggacta accattccgc ccaatgtgct ggcaagagct
     1921 gatcgagtaa tcaaataatt agacgcgagt gaaacgattt ttctgatcag attagccgca
     1981 aaatgtgagc cgatgttgaa ttggagcagc aacaatgctc cgtcacatcg ctgacgggag
     2041 gcggtttcaa atactacgag tgtgagcaga gctttcggcg cgggcttcgc cggtgaatga
     2101 aagattacga aagggcaggg tccgagaatg aacaaatatg ctatttggaa gcttctgtta
     2161 ctttcttttg cagttgcgcc actccctcgt caccatgcaa tcgttgctgc acagcagaac
     2221 accaacacga cagagacgga ggcaaaaatg ttctctgcgg ccgccgagta cgagcgattt
     2281 atgggccggt ggagccggcg gttagcgccg gaattcatcg cctttgccgg cattaaaaac
     2341 ggtgatcgtg tcctcgacgt gggcaccggg acaggatcgc tcgcctcagc gcttgagagg
     2401 acgatgccat tcagtgagat tgtcggcatc gacccctccg agggattcat cagctatgcg
     2461 aagaaaaatg ccaagtccaa tcgcacccac tttgagatcg gcgatgccca ggcgcttcga
     2521 ttccaggacg ggtcatttga caagaccatg gccctcctcg tgatgaattt tatcccgaat
     2581 catgacaggg cgatcgggga gatgcgtcgc gtgacccgtc cgcagggcgg agtcagtgcc
     2641 tgtgtatggg attatgatga aggcatgcag atgctgagat ttttctggga tgaagccgtc
     2701 gcgctaaacc cggcaattga gccaaaggac gagcgacaca tgaagctatc ccaccaaggc
     2761 gagctcgggg aactgtggaa gaaagccggg ctcgtcagta ttcaggaaaa gccgctctcg
     2821 attgatcagg catacgattc attcgacgac tactggggat ctttcttgaa gggagccgga
     2881 cccggtgggg cctacgtcgt atcgctcccg gacaaaagcc gacaggagct tgcaacccgc
     2941 ctgcgtaaac gactactcgg aaatcgcgaa aacggtccat tcgttctcaa ggcacgagcc
     3001 tggtgcgtgc gaggggaagt tcccaaatga ggcatcgaaa aatcatatga gtgtgtattt
     3061 tccgtggtta aagacatgcc ggtgggaaaa attttaaatg tagaagaacc cacgccgggc
     3121 catttttatt ggcctgatct cgatgttgat ttgagcgaag aaatcattag tcaccccgag
     3181 aggtttcctc tcaaagccaa ataacacaga aattagttcg ctctctattt ggtgattttc
     3241 tggcaagctc acgggcttgg ctgttccggg acgccgatcg accctaactt gatccgcgtc
     3301 gccttcaatc atcgataaat gctcaaggaa cgaagccggg atctttgcct tcgagcagcc
     3361 gacaaagttc gagttggtga ttggtctcta tgctgcgaat cagatcggag tgatgatccc
     3421 acaaaagttt ctggcgcgag cggattaggt gattagatga gacaaaattg gctctcaaca
     3481 aaattgatcg gctgcatcta cttactcccg tttcttttta tggtctcact tcaaccagcg
     3541 ttagctcagc aacccaaaaa gacgccacgc gtcggcattc tcagagtggg cagcccgccg
     3601 gatgttttta tcgatacgct tcggcaaggt ctgcatgacg ctgggtatat tgagggacag
     3661 aatatttttc tcgagtaccg ttggctgaag cgcgaagacc agtttgccga agccgctgaa
     3721 gacttggttc gactcaatgt cgacagcatc gtcgtgacct cgacggtggg agctttggct
     3781 gcccagcgag caacacgatc cattcccatc atcgttccgg tcatgggcga cccggtcggc
     3841 agtggattgg tcaccagctt ggcgcatcct ggcggaaatc taacaggttt ctccaacttg
     3901 gcccccgagt tgtggcccaa aagactggag ctgtttaaag aattggttcc aaaactttcg
     3961 cgggttgcga tgctttggaa cgtgaataat ccggcgatgg cgaccggagc gaaaggaacg
     4021 cgagaagcgg cgagcgccat gaacattacg attcaagatc gtggcgcccg tgatcccaac
     4081 gagctcgata gtgtttttac cgctctcgaa aaacagcgcc cagatggcgt tctcgttatg
     4141 atcgatgctt tcaccctccg taactcaaaa aaaatcattg attcgatagc caacgcccga
     4201 ttgccggcaa tgtatgacga aaaaagcccg atagcggcgg gtggtcttgt gtcctatggt
     4261 cctgacttcg ttgacttgtt tcgccgcact gctatttacg tcaacaaaat actcaaaggc
     4321 gccaaaccgg cagaccttcc tgtcgagcaa cccatcaagt ttgaactcgt catcaatttg
     4381 aacgccgcgc atcaaatcgg tcttacagtt ccatcaaata ttctggccag agcggatcaa
     4441 gtcatcaaat aggcaacagc cgtgagacaa caggcacaag gcatcagtaa aaaattccaa
     4501 gcattcgctt tattcgcgtt cggtttgtct ctgctcttcg gcgcagtgat cgcacgcgcc
     4561 cagattccga aaaatatttc ccgagtcgga attctctttg tcggcggcag agaccagcct
     4621 catttagcgg cctttaaaaa cgcgctcagc gagcgcggtt acaccgaagg taaaaacatc
     4681 gtcttcgagt accgctatgg cgagggccgc tacgatcggc tgacggatct cgccgaggaa
     4741 ctcgtgcgcg atcatgtcga cgtgatcgtc accacatcct ccgtcagcgc cgtggctgtg
     4801 cgcaaggcga cgcgaactat cccaattgtc atgacgagcg gtaatccgct cgaacagggt
     4861 ctcgccgcga gcctcgcgca gcccggtggc aatgtgaccg ggcttagctt attcgtcggc
     4921 gatctcagcg gtaagcgcgt cgagcttctc aaagaggctt ttcctgacct gacgcgtgtc
     4981 gcctgtctct ggagtccccg tgaagatcca tccacaaccg ctttcaaaga gacggagcga
     5041 gccgcgaagg cgatttcaat ccagctccac tcgatcgaac tgcgcaacgc agctgatatc
     5101 gacaaggggt tcgccgtctt accacggtcg gtcaaggcgg tgtttggtaa ccatgaatcc
     5161 gcttgtgaca ctccattcga aggtcatcgt tgagctggcg cttaaacatc ggctcgtcgg
     5221 tatgtatcca acgcggcaat ttgccgaaga aggcggattg ctcgcctacg gcccgctgat
     5281 cagcgatctt tatcgccgtg cggcgatcta cgtcgataaa attctgaacg gagcaaaacc
     5341 agccgaccta cctatcgagc aaccaacgaa attcgagttc ctggcgaacc tcaaaaccgc
     5401 ccggcagatc ggcgtcacca tcccgccaaa tgtgttggcg cgtgcagacc aagtcatcaa
     5461 atgacagtgc tgaggaacga gtgctaagga ctgaaccatg agaatcgaga tgcctgcgtt
     5521 aaactcagcg ctcatagctc agcgctcagt cctttgtgcg gcggtctgcg ctctgcactt
     5581 tgcgctttgc tcagtcgccg aggcgcagca gccggtaaaa gtggcgcgaa tcgcctacct
     5641 ctctggtggt tcactttcta atctcggggg ccgcatcgac gca
//
LOCUS       DASULZ010000280         3534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1251896, whole genome shotgun sequence.
ACCESSION   DASULZ010000280 DASULZ010000000
VERSION     DASULZ010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251896"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..527
                     /gene="rpsR"
                     /locus_tag="VFY96_05885"
     CDS             234..527
                     /gene="rpsR"
                     /locus_tag="VFY96_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX5606024.1"
                     /translation="MAQMERGPRPQRERSDDDKGPPRRRPMFRRRVCRFCADKSLAID
                     YKDLRVISQFITERGKMTPSRITGNCARHQRMLTTAVKRARSVALLPFTTAKS"
     gene            548..1498
                     /locus_tag="VFY96_05890"
     CDS             548..1498
                     /locus_tag="VFY96_05890"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HEX5606025.1"
                     /translation="MPKLEVISKFTLALASSVVLFLSGISLPPIGVIAFPFVAQPVLL
                     FGLKYGITGGIGVLCVAILLFVVFGTKELALVYGVFAALAGLLLGLLGRIRAIEYLVI
                     VIATVMLAAAGIIALQSYGSWTLMSQDVHRGLTEQVMAAIRMQENMGLSPDSLDLLKT
                     QMPLMIDRIVQLLPALVFLSFAFIILINILYLLRRFPERRGQWFSLESLREWKAPEQL
                     VWALIACGFILFIPGLVSIRNIALNLLLVIGAFYFAQGLAVIGFFFAKNKVPRFLRGL
                     TYVLIVFQQIFTLLVVGLGLFDLWGDFRRLRKDKLTSHAS"
     gene            1510..1956
                     /gene="rplI"
                     /locus_tag="VFY96_05895"
     CDS             1510..1956
                     /gene="rplI"
                     /locus_tag="VFY96_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX5606026.1"
                     /translation="MEVILKEDIANVGKIGEVVRVRDGYARNYLLPRGLVLIANKKNL
                     KTFEHQKKLVADQKQKVLRQAQGAADQLGSVSLVIAMKVGEEGRLFGSVTNIQIEKAL
                     KAKGVNIDRRKIHLDEPIKVAGDYEVPVRLAADLTVPLKISVVSES"
     gene            2199..3068
                     /locus_tag="VFY96_05900"
     CDS             2199..3068
                     /locus_tag="VFY96_05900"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX5606027.1"
                     /translation="MPQSINITARGYWLIFSAAVLWSLAGIFIKYLDVPPLAIVFYRS
                     FFAFLVFTPLLRRNQWQVNWPVLVSVLTYSAAISAFVAANKLTTAANAIVLQYTAPIF
                     VFLFSRLVLGEKISRLNGIALFSAMVGVCAISVESVGRPEMSGVMLALSSGVLFAAYM
                     INLRNAQAVNAVYLTWINNLGCALLLVLFVKSQLSLSLNELVILAVMGAVQLGLPYFL
                     FSKGLQSVPLQEASLIALIEPVLNPVWVALAFGEIPSAATFAGGGLILFGLAVRYLAP
                     WFGSSSEPVRLAS"
     gene            complement(3086..>3534)
                     /locus_tag="VFY96_05905"
     CDS             complement(3086..>3534)
                     /locus_tag="VFY96_05905"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5606028.1"
                     /translation="RIPLTGGTMPIEIMDMPKMAREEGKRKVIFNTNRFHAWLHVYPK
                     PGDADDMHCHNADQTFYVIEGECTMHFPDGGKSVMTPGMVATITGGSFYQLENTGHGS
                     MVLMGNRSGPSEAIQHINYELRKDIKTLSREEMEKIRHPGNIRVSK"
BASE COUNT          730 a          877 c          970 g          957 t
ORIGIN      
        1 aagagctttc tgcaaaaagg agctcaagtg ctggtcgacg gccggctcag tcaaagcgct
       61 gggaaaccgc ggaaggtaag ttacgcagca aatacgaagt gattgctact acggtgcagt
      121 ttttgagccg gagcaactcg actggacagg accccgccaa cgatgatgat cggttataga
      181 gaagcggtta taaaggtcgg ctccagtcaa aactactgac gaggtattaa acaatggcac
      241 aaatggaacg aggtccaaga ccacaacggg aaagatcgga cgatgacaag ggaccgccgc
      301 ggcgacggcc gatgtttcgc cggcgagtgt gtcgattttg cgcggataaa tcgctcgcga
      361 tcgattacaa agatctgcgg gtcatttccc agttcattac ggaacgggga aagatgacgc
      421 ccagccggat taccggcaat tgcgctcgtc accagcgaat gctgacaact gccgttaaac
      481 gagcgcgcag tgtcgccttg ctaccgttca ccaccgcgaa aagctaatct attttgcgct
      541 tactttgatg cccaagttag aagtcatcag caaatttact ctcgctttgg cttctagcgt
      601 ggtgctgttt ctgagcggca ttagcttgcc gccgatcgga gtgatcgctt tcccgttcgt
      661 ggctcagcct gtactgttgt ttggtctgaa atacggcatc accggcggca tcggggtact
      721 ctgtgtcgcc attttgcttt ttgttgtttt cgggacgaaa gagctggcgc tggtctatgg
      781 tgtattcgct gcgctggcgg gtctgttgct gggtttgctc gggcggattc gcgcgatcga
      841 atatttagtg atcgtgatcg ccaccgtgat gctcgcggcc gcgggcatca tagcacttca
      901 atcgtacggt tcatggacgc tgatgtcgca ggacgtgcac cgcggattga ccgaacaggt
      961 gatggccgcg atccgaatgc aagaaaatat gggcttgtcg ccggatagtt tggacctgct
     1021 gaagacgcag atgccgctca tgattgatcg cattgttcag cttctcccag cactggtctt
     1081 cctgagcttt gcctttatca tattgatcaa tattctctat ctgctaaggc gttttcctga
     1141 gcgtcgagga caatggtttt ccctcgagtc tttgcgtgag tggaaagctc cggaacagtt
     1201 ggtatgggcc cttatcgctt gcggttttat tcttttcatt ccgggtttgg tttcgatcag
     1261 aaacatcgca ctaaatctgt tgctcgtaat tggtgcattt tacttcgctc aaggactggc
     1321 ggtgatcgga ttttttttcg ccaagaacaa agtgccccgg tttttgcgcg gcttgaccta
     1381 cgtgttgatt gtttttcagc aaatttttac gttgttggtg gtcgggctgg gcttgttcga
     1441 cctttggggt gacttccgtc ggctccgaaa ggataagctc acctcgcacg cgtcctagct
     1501 ggaggctcta tggaagttat tttgaaggaa gatatcgcta atgtcggcaa aattggcgaa
     1561 gtcgtgcgag tccgtgacgg ctacgcgcgc aactatcttt tgcctcgtgg tctggtgctg
     1621 attgcgaaca aaaagaatct caaaactttc gagcaccaga aaaagctcgt ggccgatcaa
     1681 aagcaaaaag tgctgcgtca ggcacagggc gccgccgatc agcttggctc ggtttcgttg
     1741 gtcatcgcca tgaaggtggg cgaagagggc aggctttttg ggtccgtcac taacatccaa
     1801 atcgagaagg cgctgaaagc caaaggtgtg aatatcgacc ggcgcaaaat tcatctcgat
     1861 gagccaatca aagttgccgg tgattacgag gtgccggtcc gtctcgcagc ggatctcacg
     1921 gtgccgctca agatttcggt ggtgtcagaa agctagcctc ggaattccac tcgttccatc
     1981 aattcgatgg gaaccaagag ggggtgagaa tttctctccc ctcttttttt gtgccgagtt
     2041 ttagagcccg tctggatggc gatcgctcac cacaaaagct ttgtccaccg tgcgttaagc
     2101 gtctgtgaga aacacgttgt gctgcgaaac taaaaattca ttgcacccca tgcacattgt
     2161 tgttacggtt caccggttta cgcgctagcg ccagagcaat gcctcaatcc attaatatca
     2221 ctgcccgcgg ttattggctg attttctcgg cggcggttct ctggagcttg gccggcatat
     2281 ttatcaagta tctcgacgtg cccccgcttg cgatcgtgtt ctacagaagc ttttttgcgt
     2341 ttctggtgtt cacaccttta ttgaggcgaa accagtggca ggtaaactgg ccggttcttg
     2401 tctcggtcct gacttacagc gcagccatta gcgcgttcgt ggccgctaat aagctgacga
     2461 ccgccgccaa cgcgattgta ctccagtaca cagccccgat tttcgtattt cttttttctc
     2521 ggctcgtctt gggagagaaa atctcgagac tcaacggcat cgcacttttt tcggctatgg
     2581 ttggtgtctg cgccatttcc gtcgagagcg ttggccgacc ggaaatgagc ggggtgatgc
     2641 tggcgttatc aagcggcgta ctgtttgccg cgtatatgat caatttgcgc aatgcgcagg
     2701 cggtgaatgc ggtttatctg acgtggatca acaatcttgg ctgcgcgctg ctactggtgc
     2761 tctttgtaaa gtcacagttg tcgctaagcc taaatgaact cgtgattctt gccgtaatgg
     2821 gcgcggttca gctcgggctc ccatacttct tgttctccaa aggattgcag tcagttccgc
     2881 tgcaggaagc atcgctcatt gcgctgatcg aacctgtgct gaatccagtt tgggtggcgc
     2941 tggcttttgg cgagatcccc tcagccgcga cgtttgccgg tggcgggtta attctgttcg
     3001 ggcttgccgt acgttaccta gccccttggt tcgggagctc aagtgagcct gttcgtctgg
     3061 cttcgtagat ttcgagtttc ttgactcact tggagacgcg aatatttccc ggatgtcgga
     3121 tcttttccat ctcctcacgt gaaagagttt tgatgtcctt gcgcaattcg taattgatgt
     3181 gctgaatcgc ttccgaagga ccggagcgat ttcccatcag caccatcgaa ccgtgtccgg
     3241 tgttttccaa ttgataaaag gaaccgccgg tgatggtagc gaccataccg ggcgtcatca
     3301 ccgatttgcc gccatcagga aaatgcatcg tgcattcacc ttcgatgacg taaaacgttt
     3361 gatccgcatt atggcagtgc atgtcgtcgg cgtctcccgg cttgggatag acgtggagcc
     3421 atgcatgaaa gcggttggtg ttgaagatta cttttcgttt tccttcctct cgcgccatct
     3481 tcggcatatc catgatttcg atcggcattg ttcctccagt gagcggaatc cgac
//
LOCUS       DASULZ010000281         2410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1048295, whole genome shotgun sequence.
ACCESSION   DASULZ010000281 DASULZ010000000
VERSION     DASULZ010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048295"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VFY96_05910"
     CDS             <1..506
                     /locus_tag="VFY96_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606029.1"
                     /translation="RIFDRKVPADDPFNYIAGLDEKVAAWNELIQQYRATRYEYLARV
                     EEERIDRAKVAFVEANRYRLKEGNQLTILGYGQLVTKHQQSKNRYRFLLDFGDFYMLL
                     ANEYASQYDPEGLAFEFKILDQFVKSALKLYSEVAQVDGILEKVEAQGKIEAARGFLE
                     KMTRLNR"
     gene            503..>2410
                     /locus_tag="VFY96_05915"
     CDS             503..>2410
                     /locus_tag="VFY96_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606030.1"
                     /translation="MKAKNFLALLSLLVCMAFCIPYSAAQDLSLEKILNPLPDYNPFD
                     QSTAPTPQFFPDEVDKHARDLLIDALIDDEQALHRHLQFFKTQDARLQKERGTSTGLT
                     AQAQDLVNNTIRDRARYLAAQNDALKNAATPTRKKYLEAIINNDDLNQSDQLMRQSST
                     NFWGGMGNRLLSSVDLIGVASGNYVGAVAETAVSQLYGLMDRDMSIEERRALARDLEH
                     LNRFPDDPRNAPIKKQIEQLEKKKKTALLRKQIDRAKEAWGIGDFDRALFHLDLAAFI
                     EPESKDSTELREKVTKAIKDTDAARQKSLVAQAESPASDEQHADVKRLLEALSLRDSN
                     QLQRIAIETEKKYLGTPLADAARDSEAVALEMKGWREAAKKALQQMANSTKNPEIKQR
                     ANVLLQSPEYNLLTPFQEARSERELQSAKYVLFGEDFLRKNVLFAAGAMAAAGPGAAV
                     TLAMANALIITNNFYQVMTQNPVSAQSVIDAGVAYVRNHPDSENASEVYRVLADAYEE
                     RGMLDQAINYHQLAGSSKEKIAALKEKSAKMLLDAAAKTSNRSSRKYYLTTVVDEHPE
                     TPAAREAIRRLAELVKHENQGLRMSKQFLLEHPELHGARGLGLKPALFDGRSENMEIA
                     DRGVNLISDNEI"
BASE COUNT          642 a          660 c          630 g          478 t
ORIGIN      
        1 tgcgaatctt cgatcgcaaa gtccccgccg acgacccgtt caattacatc gccggtctcg
       61 acgagaaggt cgccgcttgg aacgaactca ttcagcaata cagggcgacg cgctacgaat
      121 atcttgcccg cgtcgaagaa gagcgcattg atcgcgccaa agtggcattc gtggaagcca
      181 atcgatatcg gctgaaagaa ggcaatcagt tgaccatcct gggttacggc caactggtca
      241 ccaaacacca gcaaagcaaa aaccgctatc gttttctgct cgacttcggc gatttctaca
      301 tgctgcttgc aaatgaatat gcatcccagt acgatcctga agggttagcc tttgagttca
      361 agattttaga tcaattcgtc aagtccgcgc tgaagcttta cagcgaagtg gcacaggtgg
      421 atggtatctt agaaaaagtc gaagctcagg ggaaaattga agcggcgcgt ggatttttgg
      481 agaaaatgac ccgactcaat cgatgaaggc aaaaaatttt ctcgccctcc tttcactgct
      541 cgtttgcatg gcattttgta ttccttactc cgcggcacag gatctgtcac tggagaaaat
      601 tctgaatccc ctgcccgatt acaatccctt tgatcaatcc actgcaccaa cgccccaatt
      661 ttttcctgac gaagtcgaca agcacgcccg tgatctgctc atcgatgccc tcatcgatga
      721 cgaacaggca ctccatcgcc atctacaatt tttcaaaacc caagatgctc gtctacaaaa
      781 agagcgcggc acctcgacgg gattaaccgc acaagctcag gatctggtga acaacaccat
      841 tcgcgatcgc gcgcgctatc tggcagcgca gaacgacgcc ctaaaaaacg cagcgacgcc
      901 gacgcgaaag aagtacctcg aagcgatcat caataatgat gacctcaacc agtccgatca
      961 attgatgcgc caaagctcga cgaatttttg gggcggtatg ggcaaccggc tgctaagctc
     1021 ggtggatttg attggcgtcg cttcgggcaa ttacgttggc gctgtggctg aaaccgcggt
     1081 gagccaactt tatggcctga tggaccggga catgtccatc gaggagcggc gtgccctagc
     1141 gcgcgatctc gagcacttga accgttttcc cgatgatccg cgcaatgcgc cgatcaaaaa
     1201 acagatagaa cagttggaga aaaagaaaaa aacagccttg ctgcgaaagc agatagaccg
     1261 tgccaaggaa gcttggggga taggcgattt tgatagagcc ttatttcacc tcgacctggc
     1321 ggctttcatc gaaccagagt ccaaagacag caccgagctg cgagagaaag tgaccaaagc
     1381 gataaaggat acggacgccg ctcggcaaaa atccctcgtc gcgcaggcag agtcgccggc
     1441 gagcgatgag caacacgcgg atgtcaagcg tttgctcgaa gccttgagcc tgcgcgatag
     1501 caatcagctt caacgcatcg ccattgagac cgagaaaaag tatctcggca cacctctggc
     1561 cgatgccgcg cgcgattctg aggcagtcgc cctcgaaatg aagggctggc gcgaagccgc
     1621 aaaaaaagcc ctgcagcaga tggcaaactc gactaagaat cccgagatca agcaacgcgc
     1681 aaacgtgctc ttgcaaagcc ccgagtacaa cctgctcacc cctttccaag aagcacgcag
     1741 cgaacgggag ctccagtcgg ccaagtacgt tttgttcgga gaagactttc tgcgcaagaa
     1801 cgtgcttttc gccgcaggcg caatggcggc cgccggcccc ggcgctgccg tgacactggc
     1861 aatggccaac gcgcttatca ttactaacaa tttttatcag gtgatgactc aaaatccggt
     1921 ttcggcgcaa tcggtcatcg atgccggggt tgcctatgtg cgaaatcatc cggactccga
     1981 gaacgcgtct gaagtttacc gcgtgttggc cgacgcttac gaagagcgcg gcatgctcga
     2041 ccaggcgatc aactatcatc agttggccgg ctcgtcgaaa gaaaaaattg ctgcgctcaa
     2101 agaaaaatca gcgaagatgt tgctcgatgc ggccgccaaa accagcaatc gcagcagccg
     2161 caaatattat ttaaccacgg tcgtagatga gcatcccgaa acaccggcgg cgagggaggc
     2221 catacgcagg ttggcagaac tggtcaagca tgaaaaccag ggcctacgca tgagcaagca
     2281 gtttttactg gaacatcccg aacttcacgg tgcgcgcggt ttgggcctaa aaccggcgct
     2341 gttcgacggg cgttccgaga acatggaaat tgccgaccgc ggcgtgaatt tgatcagcga
     2401 taacgaaatc
//
LOCUS       DASULZ010000282         4750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_338393, whole genome shotgun sequence.
ACCESSION   DASULZ010000282 DASULZ010000000
VERSION     DASULZ010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338393"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VFY96_05920"
     CDS             complement(<1..300)
                     /locus_tag="VFY96_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5606031.1"
                     /translation="MWHSIPLTLFWFVYFGSLGIFYPYFSLYLKENAGLTGTEVGLIL
                     AISPLVGMVAQPFWGQLADRTGKRGRLLALLTLATAVGYYAVGIASGFWAIVAATA"
     gene            352..924
                     /locus_tag="VFY96_05925"
     CDS             352..924
                     /locus_tag="VFY96_05925"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX5606032.1"
                     /translation="MSAIANAKCESRRTVHTIGHSTRTLQAVIELLRQHSIEIVIDVR
                     RWPASRRLPHFNRDALAAGFKAEGIAYLWRGDLGGFRKASPDSPNTGWRVAAFRAYAD
                     FMLQPEFARIMNKMEKIAGENRAAIMCAEAVPWRCHRQLLADAFVVRGWNVRHIMEDG
                     CHEHHLTKFAAPQGTRIYYKDPATTATKLF"
     gene            complement(1203..1967)
                     /locus_tag="VFY96_05930"
     CDS             complement(1203..1967)
                     /locus_tag="VFY96_05930"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX5606033.1"
                     /translation="MSESYSASEMMIAVAARMLKGARTVFVGVGLPNIACNLARVTGA
                     PDMELIYESGVYGARPERLPLSIGDPTLVSGAVSVVSMADLFGLYLQRGLVEIALLGG
                     AQIDRYGNLNSSVIGDYSKPKTRLPGSGGACEIATNAQRIFMIMRLKRRAFVDKLDFI
                     TSPGHLNGGDSRSRLGLPGKGPEIVITDRAILNFDNAEREMQLSALYPGVSEKDVRAE
                     IGWELRCAPQLEQVSPPSVEELRLIREELDPSGMYR"
     gene            complement(1964..2893)
                     /locus_tag="VFY96_05935"
     CDS             complement(1964..2893)
                     /locus_tag="VFY96_05935"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006553722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX5606034.1"
                     /translation="MNDSTADKVVSMREAVAQYVSDGSSVAIEGFTGFICFAAGHEII
                     RQRRRNLTLIRMTPDLIYDQMIAAGTAKKLIFSYMGNPGVGSLYCIRRAVEKNVPLPL
                     ELEEYSHYGLVGRYAAGAARMPFFALRSYVGSDMLTANPLIKLIDDPYGTGKIAVVPP
                     LNPDVAILHAQRADRRGNTQIWGLVGMQKEVAFAAQHVIVVVEEIVDESIIRADPNRT
                     LIPGLIVDAVVHEPYGAHPSYVQGYYDRDNESYLAWDKLSQDHTAVTAWLDEWVYGLD
                     NFAAYLRKIGQARFDKLKPEPYPAPSVDYGRYR"
     gene            complement(2896..4260)
                     /gene="pcaB"
                     /locus_tag="VFY96_05940"
     CDS             complement(2896..4260)
                     /gene="pcaB"
                     /locus_tag="VFY96_05940"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047472 - 3-carboxy-cis,cis-muconate
                     cycloisomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HEX5606035.1"
                     /translation="MTPLDSAIFAPLFTDEEVSSLFSDGAFVRALVEVELALARAEAR
                     LIVIPPSVARRIADVQADRINIESLTKGTLRSGFPIIALVEEIRKQAGSEAAPFVHWG
                     ATTQDIMDTASILQFRSVTELFKTRLAEIVRNLSSLAHRHRNVILAARTHGQQALPIT
                     FGVKIAAWLAPLLRHLQRLDEILPRLLVVQFGGAAGTLAALGDKGVPVMRALAEELRL
                     GMPLMPWHAQRDSLVEFAGWLSLLTGSLGKMAQDIILLAQTEVGEVGESAEQGRGGSS
                     TMPQKNNPITSELIVAAARTNASLLSGMHNAQIQEQERATHGWQMEWLILPQMMLLTS
                     GVLKHARYLAGHLQADDESMRANIARANHLILAEAAVFALAQAMPKSKAEELVKKACG
                     MAMSEGKPLVLAVQELSAGEIAPGSIDWQGLAQPENYLGAANEILDRVLKAADSRVAA
                     NEPS"
     gene            complement(4484..>4750)
                     /locus_tag="VFY96_05945"
     CDS             complement(4484..>4750)
                     /locus_tag="VFY96_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004926643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HEX5606036.1"
                     /translation="GPVPGNNASVQAPHINFVIFARGMLVHAYTRMYFADEPANAADP
                     VLNSIKNKKRRATLIAGREERNGKAVYRFDIRLQGENETVFFDM"
BASE COUNT         1032 a         1399 c         1322 g          997 t
ORIGIN      
        1 tgccgttgca gcaacgatgg cccaaaaccc gctcgcgatc ccgacggcat agtagcctac
       61 ggccgtagcg agagtcagaa gcgccagcaa gcgtccgcgc ttgcccgtac gatcggccag
      121 ttgtccccag aacggctgcg cgaccatgcc gaccagcggt gaaatcgcca agatcaatcc
      181 gacctcggtg ccggtaagac ccgcgttttc tttcaggtag agactgaagt aggggtaaaa
      241 aatacccagc gaaccgaagt aaacgaacca gaatagcgtc agtggaatgg aatgccacat
      301 gggccgatga tagcatcagt tatgtagggt atcacctcgc gctgctgaat catgagcgcc
      361 attgcgaatg cgaaatgcga gtcccggcgc accgtgcaca cgatcggtca ctcgactcga
      421 acgctccaag ccgttatcga gcttttgcgc cagcattcga ttgagattgt catcgatgtt
      481 cgccgctggc cggcgtcgcg ccgtttgccc catttcaacc gcgatgcttt agccgctgga
      541 ttcaaagcgg aaggcatcgc ttatctctgg cgcggcgacc tcggcggctt tcgcaaagcg
      601 tctcccgatt cgccgaatac cggttggcgg gttgccgcgt ttcgcgctta cgcggacttt
      661 atgcttcagc cggagttcgc gcgcatcatg aacaaaatgg aaaaaatcgc cggagagaat
      721 cgcgcggcga tcatgtgcgc cgaagccgtg ccgtggcgct gtcatcgcca gttactcgca
      781 gatgcttttg tcgtgcgcgg atggaatgtg cgtcacatca tggaggatgg ctgccacgaa
      841 catcacctga caaaattcgc ggcaccgcaa ggcacacgga tttactacaa agatccagcc
      901 accacagcta cgaaactatt ttgaaaggta ctggggctcc tctcggagcg aacaggcaaa
      961 taagaatcga tcgtcattcc aggcaagcta gcgacccgga atccaggcgc ttaggtaggg
     1021 gcgcatggca tgcgcccggg aacaaaacct ggatgccggc gttcgccggc atgacgaacg
     1081 tagcactgtg gctggaggcg acggaattaa atatctccga atgagacatt taatccttcc
     1141 ttcgataacc ggagccatgt cctgacggaa cggggtgcga ttcgcgcaaa gcgtgagatc
     1201 attcaacgat acatgccgct ggggtcgagc tcctcgcgaa tcaagcggag ctcttctact
     1261 gaaggtggcg atacttgctc gagctgcgga gcgcagcgga gttcccagcc aatttcagcg
     1321 cgcacgtctt tttcagagac gccgggatac agcgcgctta gctgcatctc gcgctcggcg
     1381 ttgtcgaaat tcaaaatcgc ccgatccgtg atgacgatct cgggtccctt gccgggcaag
     1441 cctaagcgag agcggctgtc gccgccgttc agatgaccgg gactggtgat gaagtcgagt
     1501 ttgtcgacga aagcgcggcg cttgagccgc atgatcatga aaatgcgttg cgcgttggtg
     1561 gcgatttcac aggcgccgcc gctcccggga aggcgcgtct ttggcttgct atagtcgccg
     1621 atcacggagg aattgagatt accgtatcta tcgatctgtg cgccgccgag gagcgcaatt
     1681 tccaccaaac cccgctgtag atacaacccg aacaaatctg ccatcgatac caccgagacg
     1741 gcgccgctca ccaaggtcgg atcgccgatc gagagcggca agcgctccgg gcgcgcgcca
     1801 taaacacccg actcgtaaat caactccata tcgggagctc ccgtgaccct cgccagattg
     1861 caggcgatat tgggaagacc gactccgacg aaaaccgttc gcgctccttt caacatgcgc
     1921 gccgccacgg cgatcatcat ctcggatgct gaataacttt cgctcatcga tacctcccgt
     1981 aatcgaccga aggggccggg tacggttccg gcttgagctt atcgaaacgc gcttggccaa
     2041 tcttccggag gtaagcagcg aagttatcaa ggccgtaaac ccattcgtcg agccacgcgg
     2101 tgaccgccgt gtggtcctga gaaagcttgt cccaggcgag ataagactca ttgtcacgat
     2161 cataataacc ctgaacatac gaaggatgcg caccgtaggg ttcgtgcacc acggcatcga
     2221 cgatcaatcc cgggatcaat gtgcgattgg ggtcggcgcg aataatcgac tcgtctacga
     2281 tttcttcgac caccacgatc acatgctggg cggcgaatgc gacttctttc tgcatgccga
     2341 ctaggcccca aatctgggtg ttcccgcggc ggtccgcgcg ctgcgcatgc aggatcgcca
     2401 cgtcaggatt cagcggcggt acgacggcaa ttttccccgt gccgtaagga tcgtcgatca
     2461 gcttgatgag cggattggct gtgagcatat cggaaccgac gtagctgcgg agagcgaaaa
     2521 agggcatgcg cgcggcgccc gctgcataac gcccgaccaa tccgtaatga gagtattcct
     2581 ccagctccag cggcaacgga acgttttttt ccaccgcgcg gcggatacag tacaacgatc
     2641 ccacgccggg attacccata tagctgaata tcagtttctt tgccgtccct gcggcgatca
     2701 tttgatcgta gatcagatcc ggtgtcatgc ggatcaaggt gaggtttcgg cgccgttggc
     2761 gaatgatctc atggccggcc gcaaaacaaa taaacccggt aaagccttca atcgcaaccg
     2821 agctgccatc gcttacatat tgggcgacgg cttcgcgcat agaaacaacc ttgtccgctg
     2881 tgctgtcatt catattcaac tcggctcgtt tgcggcaaca cggctgtcag ccgccttcag
     2941 tactcgatcg agaatttcgt tcgcggcacc gagatagttt tccggctgcg cgagaccctg
     3001 ccaatcgata gatccggggg cgatctctcc tgccgagagt tcttggaccg ccagaaccag
     3061 cggtttgccc tcgctcatgg ccatgccgca ggctttcttg accagctctt ccgcttttga
     3121 cttcggcatc gcctgggcga gcgcaaatac cgcggcctcg gcgagaatga ggtggttggc
     3181 ccgcgcgatg ttcgctcgca tgctttcatc gtcggcttgc aaatggccgg caagatagcg
     3241 cgcgtgcttg agcaccccac tggtgagaag catcatctgc ggcaggatca gccactccat
     3301 ttgccaacca tgcgtcgccc gctcctgttc ctgaatttgc gcgttatgca tccctgacaa
     3361 tagtgaagca ttggtccgcg ccgccgcgac aatcagttcg ctagtaatcg gattgttctt
     3421 ttgcggcatc gtgctgctac cgcctcgccc ctgttccgcc gactcgccca cttcgccgac
     3481 ctcggtttgc gccaaaagga taatatcctg cgccattttg cccagacttc ctgtgagcag
     3541 actgagccag ccggcaaatt cgaccagcga atccctctgc gcgtgccaag gcattagcgg
     3601 catgcccagc cgaagctcct cggcaagcgc gcgcatcacc gggactccct tatctccgag
     3661 ggctgccagc gtcccggcag caccgccgaa ttggaccacc agcaaacggg gaagaatttc
     3721 gtccaaacgc tggagatgac gcaacagagg cgcgagccat gcggcgattt tcacaccaaa
     3781 ggttatcggc agagcctgct ggccatgcgt ccgtgcagcc agtatgacgt ttcggtggcg
     3841 atgagcgaga gagctcagat ttcgcacgat ttcggcaagc ctggttttga acaactcagt
     3901 gactgatcga aactgcaaga tagaggcggt gtccatgatg tcctgcgtgg tcgcgcccca
     3961 gtggacaaac ggcgcggctt cgctgcctgc ctgtttccgt atttcttcga ccaaggcaat
     4021 aatcggaaac ccggagcgca gggtcccctt cgtaaggctt tcaatgttga tcctgtccgc
     4081 ttgcacgtcg gcaatccgcc ttgcgacgct tggcggaatt acaattaagc gcgcctcagc
     4141 tcgcgcaagt gcaagttcta cctccaccag cgcgcggacg aaggcgccat cactgaataa
     4201 gctggagact tcttcgtccg tgaagagcgg agcgaaaatg gctgaatcga gcggtgtcat
     4261 taaaagatta gcttaaattc gcttctgtca ttccgaacaa tgtgaggaat ctttcttcaa
     4321 gtcccaacac cggaaagatt tctcccggag tttaccctga gctttcgagg ggtcgaaatg
     4381 acaggctgtg aagtgcatca ttgcgcgaga actatccagc tcggtgcggg cgtttcccat
     4441 tactccgcta ctccaatact ccattactcc agcttcggct ccctcacata tcgaagaaca
     4501 ccgtctcgtt ttcgccctgc agacgtatat cgaaacgata gacggctttg ccgttccgtt
     4561 cttcgcgccc ggcgatcaga gtggcacggc gctttttgtt cttgatcgaa ttgaggaccg
     4621 gatcggctgc attcgccggc tcatcggcaa aatacattcg ggtgtatgcg tggactaaca
     4681 tgccgcgggc gaaaatcacg aagttgatgt gcggcgcctg aaccgaagcg ttgtttcccg
     4741 gcaccggacc
//
LOCUS       DASULZ010000283         2910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_35265, whole genome shotgun sequence.
ACCESSION   DASULZ010000283 DASULZ010000000
VERSION     DASULZ010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35265"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..1284
                     /locus_tag="VFY96_05950"
     CDS             268..1284
                     /locus_tag="VFY96_05950"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5606037.1"
                     /translation="MSKPAIDHRERLEELNSKLADLEMRGYWQLRRDSERMEPKLWRW
                     ADIYPALMKTAEVVRLGSDAFRRNVGLQTGSRTISMGFQIVLPGEVAPPHRHTNTALR
                     FVIKGGGAYTTSNGEPMPMYPGDLLIQPNWAWHDHVNHSDQPIIWIDALDTGLVNFLS
                     ASFREEWSEGTNQPLTKKDGASRRLYGPTRQPALQYEGAGVPYHYKWGETLEALQEVA
                     DQGIHDPYDGVLLEYKNPVTGGHTFLTMTCHLQMLLPGEETRFHRHTGTTLYHTVQGQ
                     GATEIDQEKPTKFVWDEHDSFSLPSWRWHRHRNRSATEPAILFSLTDRPVLKMTGLDR
                     EELA"
     gene            complement(1690..2853)
                     /locus_tag="VFY96_05955"
     CDS             complement(1690..2853)
                     /locus_tag="VFY96_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606038.1"
                     /translation="MSIPYTPADAAVAINDMLDCCAVVKPGQNVLILAAIDGLHGGRN
                     LVDEQTILWIQSGVQARGAHPTVIWADMPSRPNVIWPDIPTPETAWKIPPIVKAAMKG
                     ADLLINHISDLSSEEHLKEFPELLKELKLPMVRNMATTTGLLMSDWARTPHDLIAEIR
                     YRMAELIEPDKTWSLIHPNGTHLEGKVGSPGRGGEGYAYWRRDGYYRPFPDGIYPAVN
                     PVGTNGIYVFDQMMPVWAKEIGVPIRFSAPVRISVEDNQMTKIEGGAEAAAIRGFLEA
                     LARRIGEKNAFEIRGPHGGVHPHATVSPAQCPDEEYREFVASFHTGAVHMHLGQAGKS
                     ASFPFNLHTAAEARGATFKVGSKVLHDRGRLAVADDPRVKAVAARYAGRPGLQ"
BASE COUNT          633 a          832 c          809 g          636 t
ORIGIN      
        1 gagttgtggt tggaaaagat ctccggtgga gtttaccaaa actgcagaaa cttcgaatat
       61 taaattgctt attggttgtc atgtcgaacg cagcgagaaa tctttctcga tgctaatcgt
      121 cacaacggct tgatactcgt cgataccgag ttgcgtggat gctttggact ttgataaaag
      181 ccacacgggt gcgcttcttt cgaacggctt gaacgatttg aacgttttga acggctggaa
      241 cttctttcag tataaggagc cgcagatatg agtaagccag cgatcgatca ccgcgagcgg
      301 ctggaggaat taaacagcaa gctcgctgat ctggagatgc gcggttattg gcagcttcgt
      361 cgcgatagcg agcggatgga accgaagctt tggcgttggg cggatattta cccggcgctg
      421 atgaaaaccg cagaggtagt gcgactaggc tccgacgctt tccgccgcaa cgtcggcctg
      481 cagacgggca gtcgaacgat cagcatgggg tttcagatcg tcctgcccgg agaagtcgca
      541 ccgccacatc ggcacaccaa caccgcattg cgcttcgtga ttaaaggcgg cggagcctac
      601 accacgtcca atggcgagcc aatgcccatg tatccaggag atcttctcat ccagccaaac
      661 tgggcttggc acgaccacgt gaatcattca gatcagccga tcatttggat cgacgcgctg
      721 gataccggtt tagtgaattt tttaagcgct tcgttccgcg aggaatggtc cgaaggaacg
      781 aatcagccgt tgacgaaaaa agatggagct tcgcgacgac tctatggtcc gactcgacag
      841 ccggcgctcc agtatgaagg ggctggcgtt ccgtaccact ataaatgggg agagaccctc
      901 gaagccttgc aagaggttgc cgaccaagga atacacgatc cttacgatgg cgttttgctg
      961 gaatacaaaa atcccgtcac cggaggtcac accttcttaa ccatgacttg ccacctgcaa
     1021 atgcttctgc ccggtgaaga aacccgcttc catcgccaca cgggaacaac tctgtatcat
     1081 actgtgcaag gacaaggcgc cactgaaatc gatcaagaaa aaccgactaa attcgtctgg
     1141 gacgaacacg acagtttcag cctgccgtca tggcgctggc accgccaccg caatcgctcg
     1201 gcaactgaac cggcgatcct tttttcatta accgatcggc cggttctgaa gatgacggga
     1261 ttagaccggg aggagcttgc ttaatcagat cgttacaaac gcattggagg attaattgcc
     1321 attccgaacg gatgtgagga atccagattt ctccctcctg tcaaaatgac agtcggatcg
     1381 atacacacca gcgtttgact tgtcgatcta tgctcgaagc gacggaatgt tctactttcc
     1441 catttcagcc agactatctt tgattaattg cagcgatgga ttgcgatgag agagtttctg
     1501 ttcgtccaga tccatataat tgacaacgtt ctgtctccga tccggcagaa ccggctcccg
     1561 gcgatgaccc ttacctagac tggtaaaaat tatcgcgtgg tggcagcatt acgtgaatgt
     1621 tttctcacgg gcggccgatt agcaggcacg gacatgcctg gccttggtcc aacccgcaga
     1681 ggtcgacttc tactgaagac ccggtcgtcc agcgtagcgc gccgccaccg ccttcacgcg
     1741 gggatcgtcg gcgaccgcga gccgcccgcg gtcatgcaat actttgctgc cgaccttgaa
     1801 cgtcgcgccg cgcgcttccg ccgctgtgtg cagattgaac ggaaacgaag cactcttgcc
     1861 ggcctggccg aggtgcatgt gcaccgcacc ggtgtggaac gaggcgacga attcgcgata
     1921 ttcctcgtcc gggcactgcg cgggcgacac ggtcgcgtgc ggatgcacgc cgccgtgcgg
     1981 tccccggatt tcaaacgcgt tcttctcccc gatgcgccgc gcgagagctt caaggaatcc
     2041 acgaatagct gccgcttccg cgcctccctc gatcttcgtc atctgattgt cttcaaccga
     2101 gatgcgcacc ggcgcagaga aacggatcgg cactccgatc tccttcgccc agaccggcat
     2161 catctgatcg aacacataga tgccgttggt cccgacagga ttcaccgctg gatagatgcc
     2221 gtccgggaac ggacgatagt agccgtcgcg acgccagtag gcatagcctt cgccgccacg
     2281 ccccggcgac ccgaccttgc cttccagatg cgtgccgttc gggtggatta ggctccacgt
     2341 tttgtcgggc tcgatcagtt ccgccatgcg gtagcggatc tcggcaataa gatcgtgcgg
     2401 cgtgcgcgcc cagtcgctca taagcagacc cgtcgtggtc gccatattgc ggaccatcgg
     2461 cagcttcagc tccttgagga gctccggaaa ttccttgagg tgttcttcgg acgagagatc
     2521 gctgatgtga ttgatcagca gatcggcgcc cttcatcgcc gccttgacga tcggcggaat
     2581 cttccaggcc gtctcaggcg tcggaatatc gggccagatc acattcggcc ggctcggcat
     2641 gtcggcccat ataacagtag gatgcgcgcc gcgcgcctgc actcccgact gaatccatag
     2701 tattgtctgt tcgtcaacaa gattgcggcc gccgtgcaat ccgtcgatcg ccgcgagaat
     2761 cagcacgttc tggccgggct tcacgaccgc gcaacagtcg agcatatcgt tgatcgcgac
     2821 tgcggcgtcc gccggcgtgt aagggatgga caatggcatt cctcccgaga ctgttggcgc
     2881 tatttggcgc gcagtcttcc tgttgttgcg
//
LOCUS       DASULZ010000284         2875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1048479, whole genome shotgun sequence.
ACCESSION   DASULZ010000284 DASULZ010000000
VERSION     DASULZ010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048479"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VFY96_05960"
     CDS             <1..188
                     /locus_tag="VFY96_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606039.1"
                     /translation="IFWLNDSTAVYVAELLASLALSGALSTTRCLGPHFARSGSLIRF
                     SLRRHGILPGRTAQIDE"
     gene            344..499
                     /locus_tag="VFY96_05965"
     CDS             344..499
                     /locus_tag="VFY96_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606040.1"
                     /translation="MTLATLYGYRQPITAAISKNLAALGLEKRAPAAKSLEEILNEPD
                     HDQPEPE"
     gene            801..1163
                     /locus_tag="VFY96_05970"
     CDS             801..1163
                     /locus_tag="VFY96_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606041.1"
                     /translation="MSDLTKHQIYEAVEVLFNTGAVLLLEHEMMESQFMGLVWRGSRI
                     DHLPNEHDDFCNAVGGAVHLASKNKTTHFGFPIAVGHVPKFGDGNAQDSRGGYYVGAR
                     PWPTGNPRGVDPWGKLPK"
     gene            1396..2610
                     /locus_tag="VFY96_05975"
     CDS             1396..2610
                     /locus_tag="VFY96_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606042.1"
                     /translation="MRKFVAVILLSTACLAVAVPALTFAQTTLPPACEEGSLPSGDQK
                     FPASQLILVCIPANWNGNLVLYAHGYVPPKPEFPLVLPIAELTLDGTFFPQLLLTQGF
                     AFATSSFHKNGAAIQQGAEDLNDLLAHFKTLVPEKSLQNVLLIGASEGGLIALELLER
                     KPHEYDAALALCGSIGGSIEQIRYAYDFRVVFDYFFPTVFTFPPDQPGEKHFGAFDVP
                     ENAYLFWERVYMPRIVTAMTNDPLLTTELFRVTGAAIDRNDPNTAVETALNVLFYNIF
                     GTNDNIATSGGIPYDNRFKIYRGSTNDAALNRGVERIKGDLRAELYAFRFYQSTGDLH
                     QPLVMLHNTLDPAVPFRHELIYSALVALQHRTRFLTVLPVPTYGHCEFTADQILGAFA
                     LMVQQATGKIGD"
BASE COUNT          655 a          805 c          766 g          649 t
ORIGIN      
        1 agattttttg gctgaatgat tctactgcgg tctacgttgc ggaactcctg gcatccctcg
       61 ccttgtcggg tgcgctttcg accacgcgct gtttagggcc acacttcgct cgcagtggct
      121 cattgatccg cttctccttg cgtcgccacg gcattctgcc cggacgaact gcgcagatcg
      181 atgaatgatc gcccgcgcct tgtgcgtctg cggatcaaag tgcgcagact gagcagacca
      241 tgcttttctg cgttcagatt gccgttaggc gcgccgcgtc tggccatgct tgtgggggtt
      301 cccttagtca gtatccctga cggtttttaa tcctgaattt gctttgacac tggccactct
      361 ctacggctac cgccaaccga tcaccgcggc gatcagcaag aaccttgctg cgctcggatt
      421 agagaagcgc gcaccggcgg caaagagtct cgaggagatc ctgaacgaac ccgatcacga
      481 tcaaccggag ccagaatgaa tacaccgggc ggaatagccg tcgcgtgacc gacaggaagc
      541 gcaattgatg gaaaagaagc aggccaaaca gttgaagcgg gaatagtgca ggcagacatc
      601 atcgatcatc aaacgccttg cgagattgtt ggattcatta ccggatgccg aggtgctgga
      661 aatcctccgg aatgcgaaat aagtgcgaat ttgtattttt atcgtggcat gtcgcaggac
      721 ttcaggttac cccaagatgg tcaacagcat gcagcttggg ttgggttctc gccttcgaga
      781 aacagcggat aagttaccag gtgagtgatc taaccaagca ccagatctac gaggcggtcg
      841 aggtgctgtt caacaccggc gctgtattgc tcctcgagca cgagatgatg gagtcgcagt
      901 tcatgggact ggtttggcgc ggcagcagga tcgaccattt gccgaacgag catgacgatt
      961 tctgcaatgc cgtgggcggc gctgttcacc ttgcgagcaa gaacaaaaca acgcatttcg
     1021 gatttccgat cgccgtcggg cacgtgccaa aattcggcga tggcaacgcc caggacagcc
     1081 gcggcggata ctatgtcggc gcaagaccgt ggccgacagg caatcctcgc ggtgtcgatc
     1141 cgtggggaaa attgccaaaa tgactaaagc cctccacagc gcgagtttgg gaggacggca
     1201 cgatttcaaa aaccgttgga cgaaggctgt gacgtgcgat atataatcgc ctcgcgagcc
     1261 taacatctag gattcgactc ccgaaatagc agccggtctc cctatgggtt ctgtaacaga
     1321 ccggacgagt acaacaacaa cattatgcgc agctcgtggt gtaactgctg tgaattcggc
     1381 tgaaaaggac ttgtggtgag aaagtttgta gccgttattt tgctttcaac ggcctgcctg
     1441 gctgtagccg tgccggcgct cacgttcgcg caaaccactt tgccacccgc atgcgaggag
     1501 ggttcactgc cgagcggcga tcaaaaattc ccagcatctc agctaatcct cgtctgcatc
     1561 ccggcaaatt ggaacggcaa cctggtgctg tacgcgcacg gctacgttcc accaaagccc
     1621 gagttcccgc tcgtgctccc gattgccgag cttacgctcg acgggacctt tttcccgcaa
     1681 cttcttttga cgcagggctt cgcgttcgcg accagcagtt ttcacaaaaa tggtgccgcg
     1741 atccaacaag gggccgaaga tttaaacgat ctccttgccc acttcaaaac tctggtgcca
     1801 gaaaaatcgc tgcagaatgt attgcttata ggtgcatcgg agggcggctt gatcgcgctg
     1861 gaattgctcg aacggaaacc gcacgagtac gatgcggctc ttgcgctatg cggctccatc
     1921 ggcggctcaa tcgagcagat tcggtacgct tatgatttcc gagttgtctt cgattatttt
     1981 tttccgacgg tctttacgtt tccgccggac caacccggcg agaaacactt cggagccttc
     2041 gatgtcccgg agaatgccta tctgttttgg gagcgcgtct atatgccgcg aatcgtcacg
     2101 gcaatgacaa atgacccgct cttaacgacg gaactcttca gggtcaccgg cgcggcgata
     2161 gatcgcaatg atccgaatac ggctgtcgag acggcgctga acgttctctt ctacaatatc
     2221 tttggaacca acgataacat cgcaacaagc ggcggcatac cctatgacaa ccgattcaag
     2281 atttatcggg gctctaccaa cgatgctgcc ttgaatcgcg gcgttgagcg catcaaaggc
     2341 gatcttcgtg cagagttgta cgcctttcgc ttttaccaaa gtaccggcga cttgcaccag
     2401 ccactggtca tgctccacaa taccctcgac cccgcggtcc cattccgaca cgaattgatc
     2461 tacagcgcgt tagtagctct ccaacacaga accagattcc tgaccgtact cccagttccc
     2521 acctacggcc attgcgagtt tacagccgac caaatcctcg gcgcctttgc gctcatggtc
     2581 caacaggcca ccggaaagat cggagattaa ccggcgccca gactccggct cgaacagatt
     2641 tcagtcagat gaagagactt taaccagggg aggccaagct taattcgccc ctgctacccg
     2701 acacgaggat tttcagtcct cttgccgtag cgggactatg tgacactatc tgtacctaat
     2761 gtatctattc gagggtttaa cgcctgtttc agccggccga ttttaccgac tccaacacat
     2821 ggtgacatat agttcgggca aagttgtggg caaagtggcg gcttggcgcc atgta
//
LOCUS       DASULZ010000285         4693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1354861, whole genome shotgun sequence.
ACCESSION   DASULZ010000285 DASULZ010000000
VERSION     DASULZ010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354861"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VFY96_05980"
     CDS             <1..626
                     /locus_tag="VFY96_05980"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX5606043.1"
                     /translation="SCACGALAGIFLARGHFGFGAMCATVCGLLDSVDGMVARLSGVA
                     SDAGELLDAAIDRYVEFFFLAGLIIYYREQPALQLLALLALLGSFMVSYSTTKAEALN
                     IEPPNGLMRRPERAVYLTAGAALSTPSIAWFESTPEFATPLAYPMVLALALVSVLANF
                     SAIERFWLIAKALRRRDLKPRENASTNETKPKQTSEAEVTHPADIGL"
     gene            650..1054
                     /locus_tag="VFY96_05985"
     CDS             650..1054
                     /locus_tag="VFY96_05985"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX5606044.1"
                     /translation="MQLPLMVSLRRSQIAAISATLVDFSSLIFLVEIGRVWYVAATAT
                     GAFLGALVNFLLGRHWSFAAGDEAIHGQVFRYAAVSAGSLVLNSAGVYLLTEGFGIHY
                     ALSKLITAILVGLLFNFPLHRRFVFRRHTYAQ"
     gene            1051..2421
                     /locus_tag="VFY96_05990"
     CDS             1051..2421
                     /locus_tag="VFY96_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5606045.1"
                     /translation="MKSPIRQARQQKPTIKTVIPGPLSKALRQREDEHLAPGLQNFAL
                     LAGIAVKEAHGSAITDVDGNIFIDIIGGIGVNALGHSHPSYVQAIQAQVEKSSVGSFT
                     SEPRAKLFEQLAEHRPAPALHRVQLYSSGAEAVESALRLAKAYTGKYEFVSFSGGFHG
                     KTMGVLSLMGSDFKKGFGPMLPGCHIAPYADCYRCPLKLKYPSCGLACVEETRRQVKQ
                     NSSGKVAAFLVEPMQGTAGNVIPPKEFLPAIKEVARDFGALLIVDEMITGFGRTGEYW
                     GAWHSGVEADIITIGKQFGGGFPISGMISTDEIASAKPWSDPSGSSSSYGGNPLAAAA
                     ASASLRIIDDEALVENSKRVGEYFLERIADWVERFSFVGDVRGAGLFIGMDLVSDKNT
                     KEPLSKVSCRRIFDECLRHGLLTMTYTPRVRIQPAMTIDEGTIDGVVAILAEVFEQAD
                     REGWAK"
     gene            2409..2891
                     /locus_tag="VFY96_05995"
     CDS             2409..2891
                     /locus_tag="VFY96_05995"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEX5606046.1"
                     /translation="MGKINTIWRRAVLFCVTGGGAGYVPRLPGTVGTLVAVPFSIALN
                     RLAAEKLPLASTIVVVSVLCAIRASTRGAAILGQKDPQIIVVDEIVGFLVGNFLAPLR
                     WTVLFWSFLLFRLFDITKLFPINRLEQLPGGAGIVLDDVMAGCYTFFSLRLLLYFGWL
                     "
     gene            2982..4562
                     /locus_tag="VFY96_06000"
     CDS             2982..4562
                     /locus_tag="VFY96_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein D"
                     /protein_id="HEX5606047.1"
                     /translation="MDRRGFLKNAVILTGGLLSLENWLNTAESDTQRNRFDFASLKGY
                     ARALTAKPYKAPDRDLPAALAHLDYDGVQALRFRAPRSLWYSTGTRFRVEFFHRSSLF
                     KDRLQFAEVVNGAAHDLEYDPAMFDFSRTGVQPHALKKHLGFAGFRVHFHTDWEADVT
                     AFLGASYFRAVGTDTRQYGLSARGLAIDTALEHGEEFPIFTHFWLERPGENLDRLSLY
                     ALLDSPSVTGAYRFEIAPGPTTTMDVDAALYPRKTIERLGIAPLTSMYQYGKNDRRMA
                     NDWRPEVHDSDGLAILTGAGEWIWRPLVNSSGVRVNSFLDENPRGFGLLQRDRNFDHY
                     QDDGAYYDRRPSLWVETKLAATGWGRGAIQLVELPAVDETFDNIVAFWNPAEKPQPSQ
                     ELLFSYRLHWGTRVPLAPDRAQVVATRTGIGGVVGQKRNYFSWRFTVDFVGGGLAALP
                     PKAKVEPIITASRGEIEITSARPQKEIKGYRAMFDLKPTDSSTEPIDLRLFLQLDGEP
                     LTETWVYQWTPPPPAERN"
BASE COUNT          948 a         1270 c         1377 g         1098 t
ORIGIN      
        1 tctcttgtgc gtgtggcgct ctggcgggaa tcttcctggc gcgaggccat ttcggctttg
       61 gtgccatgtg cgccaccgtt tgcggtttgc tggattctgt ggacggcatg gtggcgcggc
      121 tttccggggt tgcttcggac gcgggcgagc ttctcgacgc cgccatcgac cggtacgtgg
      181 aattcttttt tttggcgggt ttgatcatct actatcgtga gcagccagcg ttacaattac
      241 tggcgctgct ggcgctgctc gggagcttta tggtcagcta ctcgactacc aaggccgaag
      301 ctctcaacat cgaaccgccg aatggattga tgcggcggcc tgaacgcgcg gtatatctga
      361 ccgcgggcgc tgcattgtcc acgccgagca ttgcgtggtt cgagagcacg ccagaatttg
      421 ccacgccttt ggcttaccct atggtgctgg ccctggcgct ggtatcggtt cttgccaatt
      481 tctctgccat cgagcggttt tggctgattg ccaaagcgct gcgtcggcgc gatctcaagc
      541 cgcgcgaaaa cgccagtacc aacgaaacaa aacccaagca aacctcagag gcagaagtga
      601 cacaccccgc cgacatcgga ctctgacacg gtgcggtatg cgaggtcatt tgcaactacc
      661 tttgatggtt tctttgcgcc ggtcgcagat cgccgcaatt tcggcgacgc ttgtcgattt
      721 cagctcattg atattcctcg tcgagatcgg ccgggtttgg tacgtcgccg cgacggcgac
      781 tggcgctttt ctcggagcgt tggtgaattt ccttctcggc cggcattgga gctttgccgc
      841 gggagacgag gcaattcacg gccaagtgtt tcgttacgcg gcggtttccg ccggaagcct
      901 ggtcctcaat agtgccgggg tttacctgct gaccgaaggc ttcggtatcc attatgccct
      961 ctctaaattg atcaccgcta ttttggtcgg gcttctgttt aattttccac tgcatcgccg
     1021 gtttgttttt cggcggcaca cttacgctca atgaaaagcc ccatccggca agcgcgacaa
     1081 caaaagccga caatcaaaac ggtcattccg gggccgttga gtaaggcgtt gcgccaacgg
     1141 gaagatgagc acttggctcc ggggctgcaa aattttgccc tgctcgccgg cattgcagtg
     1201 aaggaggcgc acggcagcgc aatcaccgat gtcgacggca atatttttat cgacattatc
     1261 ggcggcatcg gcgtgaacgc gctgggtcac agccatccct cctatgttca agcgattcaa
     1321 gcgcaggtcg aaaaaagcag cgtcggcagt ttcaccagtg aaccgcgcgc caagctcttc
     1381 gagcagctgg cggagcaccg tccggctccg gcgctgcacc gggtgcagct ctactcaagc
     1441 ggggccgaag cggttgagtc ggcccttcgg cttgccaagg cttataccgg aaaatatgag
     1501 tttgtcagct tcagcggcgg atttcacggc aaaaccatgg gcgttttgtc gctcatggga
     1561 tccgatttca aaaaaggctt cgggcccatg ctaccgggtt gtcacatcgc gccctacgcc
     1621 gactgttatc gatgtcctct taaactcaag tatccatcgt gcgggcttgc gtgcgtcgaa
     1681 gagactaggc ggcaggtgaa gcaaaacagc agcggcaaag tcgccgcttt cctggtcgag
     1741 ccgatgcaag gcacggctgg caacgtgatt ccacccaaag aatttttgcc ggcgatcaaa
     1801 gaagtcgccc gcgatttcgg tgcgttgctg atcgttgatg aaatgatcac cggattcggc
     1861 cgaacgggtg aatactgggg tgcgtggcac tcgggagtgg aagcggacat catcacgatc
     1921 ggcaagcagt tcggcggcgg attcccgatc agtggaatga tctccaccga tgaaatcgcc
     1981 agcgcaaagc catggtccga tccctccggg tcttcatcga gctatggggg aaatccgctg
     2041 gcggcggccg cggcgagcgc ctctctcagg attatcgacg atgaggcgct cgtggaaaat
     2101 tccaagcgag tcggcgaata ctttctcgag cgaattgcag attgggtgga aagattttca
     2161 ttcgtgggcg atgtccgcgg cgccggcctg ttcatcggca tggatctcgt ttccgataaa
     2221 aacacgaagg agccactatc aaaggtctcg tgccggagaa tttttgacga atgtctcaga
     2281 cacggtcttt tgaccatgac ctatacgccg cgggtgcgaa ttcaacccgc gatgacaatc
     2341 gatgaaggaa ccatcgacgg cgtggtggcg attctggcag aagtgtttga gcaggcggat
     2401 cgtgagggat gggcaaaata aatacgattt ggcgaagagc agtgctattt tgcgtgacag
     2461 gcggaggggc gggatatgtt ccccgccttc ccggaaccgt cggtactctc gttgccgtgc
     2521 cgttctcgat tgcgcttaat cgcttggccg cggagaaact tccgcttgcg agcaccatcg
     2581 tcgtagtgtc agtcctgtgc gcgatccggg catcgacgcg aggcgcggca attttaggac
     2641 agaaagatcc gcagatcatt gtcgtcgatg aaatcgtcgg gttcctggtg gggaactttc
     2701 ttgctccttt gcggtggacg gttctattct ggagcttttt gctttttcgc ttgttcgata
     2761 ttaccaagct atttcccatc aaccgattgg aacagttgcc tggcggagcg ggtatcgtgc
     2821 tcgatgatgt catggcgggt tgctatacgt ttttcagtct gcgtctgttg ttgtatttcg
     2881 gctggctgta agtcatcttt tagaattatt ataaatctcg ctgggctggt tgccgatcca
     2941 aacttctgcg gtcggcccga ttcttccaaa ccccttaatc aatggatcgc cgtggatttt
     3001 taaaaaacgc cgtcattctg accggcggac ttctctcgct ggagaattgg ctcaacaccg
     3061 cagaaagtga tacgcagcgc aatcgattcg atttcgcatc tctaaagggc tatgcgcgcg
     3121 cgctgacagc gaaaccttat aaagcgccgg atagagattt gcccgcagcg cttgcccacc
     3181 tcgattatga cggcgtacag gcgcttcgtt ttcgcgcgcc gcgctcgctt tggtattcga
     3241 caggaactcg atttcgcgtt gagttcttcc accgaagctc gttattcaaa gatcggctgc
     3301 agttcgccga agtcgtaaac ggtgctgccc atgatcttga gtatgatccc gcgatgttcg
     3361 atttttcgcg aacaggcgtt caaccgcacg cactgaaaaa acatttgggg tttgccggtt
     3421 ttcgagtgca ctttcacacg gattgggaag ccgacgtgac ggcattcctg ggcgcgagct
     3481 actttcgcgc cgtaggcacg gatacacgcc aatacggctt gtcggcgcgc ggtctggcga
     3541 tcgacactgc gctcgagcac ggcgaagagt ttcccatttt tacccatttt tggctcgaac
     3601 gccccgggga aaatttagat cgcttgagcc tctatgcgct gctggattcg ccgagcgtta
     3661 ccggtgcgta tcgcttcgaa attgcgcctg gaccgacgac cacgatggac gtggacgccg
     3721 cgctctatcc gcgaaaaacc atcgagcgcc tcggcattgc gccgctcacg agcatgtatc
     3781 aatacggcaa gaacgatcgg cgcatggcca atgactggcg ccccgaagtg cacgattcgg
     3841 atgggcttgc gatcttgacc ggtgccggtg aatggatttg gcgccccttg gtcaactcga
     3901 gcggcgtcag agtgaactcc ttcctcgacg aaaatccccg tggatttggt ctgcttcagc
     3961 gcgatcgcaa cttcgatcat tatcaagacg atggagccta ttacgatcgc cgcccaagcc
     4021 tttgggtcga aaccaagctg gccgcgaccg gttggggaag aggcgccatt cagttggtgg
     4081 aattgccggc cgttgacgaa accttcgaca atattgtggc tttttggaat cctgcggaga
     4141 aaccgcagcc gagtcaggaa ctcttgttca gttaccggtt acactggggc acgcgggtgc
     4201 cgcttgcgcc ggatcgcgcg caagtggtgg ccacacgcac cgggattggc ggtgtggtcg
     4261 ggcagaaacg gaattatttc tcgtggcgat ttaccgtcga tttcgttggc ggcgggcttg
     4321 ccgcattacc gccaaaggcg aaggtggagc cgattattac cgcatcgcgc ggtgaaattg
     4381 aaatcacctc ggcgcgaccg caaaaggaga ttaaaggcta tcgagcgatg ttcgacctaa
     4441 agccgaccga ctccagcacc gagcccatcg acctgcgcct ttttttgcag ctcgatgggg
     4501 agccgctgac tgaaacatgg gtgtatcaat ggacgccacc gccgccggca gaacgaaact
     4561 gaaatagacc tgatcccgtc ggggtaacgc gagcgatcca gtactcttgt ggggcgatag
     4621 catacgcccg acctgaggtg tgtccgcgct cgccttaaaa tagagctgaa gcaacagaag
     4681 tttgtatgtt gga
//
LOCUS       DASULZ010000286         2729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1152112, whole genome shotgun sequence.
ACCESSION   DASULZ010000286 DASULZ010000000
VERSION     DASULZ010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152112"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            294..1172
                     /locus_tag="VFY96_06005"
     CDS             294..1172
                     /locus_tag="VFY96_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEX5606048.1"
                     /translation="MTTEGKNAAAEEAITKEALTRYQATTGPRLREIILSLIKHLHGF
                     ARDVRLTGDEYLFAMNYLVQLGGYCKGGRQEFLALAEMLGLETLVIQISQPKPEGATL
                     PGVLGPFFLPNAPSFANGADIAEDVAGEPLYVSGRILAIDRRPVPNATINVWQSDGRG
                     LYDVQGDLAKDGMRTRGQLKSEPDGRYSFWTVVPIGYPVPNDGGMGDLVKYTSNGGWR
                     PAHVHFMIEADGHDTLVTQICVRGSEYLDRDAAFSVRDGLIADFPRHQPGKAPDGRQM
                     DRPFHTMDYDFVLTRA"
     gene            1949..2545
                     /locus_tag="VFY96_06010"
     CDS             1949..2545
                     /locus_tag="VFY96_06010"
                     /inference="COORDINATES: protein motif:HMM:NF025948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606049.1"
                     /translation="MREILTDIFTWPWFSEPHGYNFNGHFIKHPGGNLCVDPVEPSDE
                     DLSEFERHGVARILITNRNHSRAANKIRARTGARTLIHPADAAHARKEGAELDGELNP
                     GDKVGPFVVIDAPGKSLGEVVLHWPERKILLVGDVVVGDPPGKCKLLPDKVVDDPPRL
                     RDSARQLLALDFDILLVGDGVPILAGAKERLRELAATF"
BASE COUNT          593 a          768 c          838 g          530 t
ORIGIN      
        1 cggagatcat gtgggcggag cgatcgagaa cgctcaagag cgttcgaaag ctcgccaaga
       61 accaaaccag ttccgtcagg acaaaagcgc cgcccaacaa actgctgcac gcaagcgcgt
      121 taagaaacgc ggcgcgtgag cagtggcgtt aggccgaaaa cctgccacgg aaagcaagta
      181 tggaacaacc agatgccgaa ctaggcggcc gttgagaacc acttttacgg cgaggagttg
      241 agtgaggtgg aagcggccct gaaaaccgtc atcaacaatc ggaggacacg ttcatgacta
      301 cggaagggaa aaatgccgct gccgaagagg cgatcacgaa ggaagcgcta acccgctacc
      361 aagctacgac gggcccccgc ctgcgcgaga tcatcttgag cctgatcaag catctgcatg
      421 gctttgcgcg cgacgtgcgg ctgaccggcg acgagtacct cttcgcgatg aactatctgg
      481 tccagctggg cgggtactgc aagggcggca ggcaggaatt cctcgcgctc gccgagatgc
      541 tcgggctcga aaccctggtg atccagatat cgcagccgaa gccggaaggc gcgacgctgc
      601 cgggcgtgct cggaccgttc ttccttccga acgcaccctc tttcgcgaac ggcgccgaca
      661 tcgccgaaga cgtcgcgggc gagccgctct acgtctcggg gcgtattctc gcgatcgatc
      721 gtcggcctgt tccaaacgcg accatcaacg tctggcaatc ggacgggcgg ggactgtacg
      781 acgtgcaggg tgacctggcc aaggacggaa tgcggacgcg ggggcaattg aagagcgagc
      841 cggacggtcg ctattcgttc tggacggtcg tccccatcgg gtatccagtt ccgaatgacg
      901 gggggatggg cgacctggtc aagtatacct cgaacggggg ctggcggccg gcgcacgttc
      961 acttcatgat cgaggccgat ggccacgaca ccctggtgac gcaaatctgc gtgcgcggca
     1021 gtgagtacct cgaccgggac gctgcctttt cggtacggga cgggctgatc gcagactttc
     1081 cgcggcatca gcccggtaag gcgcctgacg gccgccagat ggatcgcccg ttccacacca
     1141 tggactacga cttcgtattg acccgcgcat gatcaagggc gtcatgttgg aggatgaaaa
     1201 ggtcccggtg agtcaactct aacctatgcc acggggtaag gcaggccgaa caagccgctg
     1261 cagccgaccg ggccggccat tgcggcttct gggagttcac aggtcactca ggccgcccgg
     1321 cggctgagcg gggtcgttag atcgctaaat acgagagcta aagaacgttt catgaccgcg
     1381 cgaggtgtta attgtggctc agcgatgcaa tagcatcgtg tcagtcgttg ggacgactca
     1441 tcttttgaca ttccttccaa tttagtcgct cgtgaggcaa acggtttatt tcacttgagc
     1501 gtgcccatga tttcccggta cgtgcgccgc gcggcgactt ctccttgaga tttgtatctg
     1561 catctgttcg tacgagggaa tttgattgcg gcgacgactt gatcctcgga cgctctcggt
     1621 cgatttcttt ttgaactgcg tcgcgcaaat agacgcgaaa acggtggagg ccctggcgga
     1681 tttctttggg atcttccggg aatggggccg tgcccgggca cgaagatgtc tgccggcact
     1741 ggacatcttc gtcctcctgc gtgacttagg aattaggcga gttctatgtc ggttacgctt
     1801 gtcatcattt ccgctacacc gcaacagggt atggaaacag cgagcagcat aagggatcag
     1861 gggttgcccc aaagctttga caagtgaata aaaatggtcg aatcgtaaag ccggagtctt
     1921 caaaagcaat agaacgtaag gagacaaaat gcgcgagatc ctcacggaca ttttcacttg
     1981 gccgtggttt tccgaaccgc atggttacaa tttcaacggc cacttcataa agcaccctgg
     2041 cggaaatctt tgcgtcgatc ctgtggagcc cagcgatgag gacctctccg agttcgagcg
     2101 gcacggcgtc gcgcgcattc tcatcaccaa ccgcaaccac tcgcgggccg cgaacaagat
     2161 tcgtgcgcgc accggcgcgc ggacgctgat ccatccggcg gacgcagctc acgcccgcaa
     2221 agaaggcgcg gagctggacg gtgaattgaa cccgggggac aaggtcggtc cgttcgtggt
     2281 gatcgacgcg ccgggaaaat cactgggcga agtggtactt cattggccgg agcgaaaaat
     2341 tcttcttgtc ggtgacgttg tcgtcggcga tccaccgggc aaatgcaagc tcttgccgga
     2401 caaggtggta gacgacccgc cgcgcctgcg tgacagcgca cgtcagctac tagcgctcga
     2461 cttcgacatt ctactggttg gcgacggcgt cccgattctc gcgggagcga aggagcgatt
     2521 gcgggaattg gcggctacct tctaaagcca aaaagctcca gcgttcaaga gcttgggcgc
     2581 tgaatatcat ttcgattcgc gaaaaactcc tgccaatttt gggcgcgact ccgcatttta
     2641 tcgctacggt aacgcaggcg ctgcttcgct tggctggtca taacgggtca gttgtcgcac
     2701 cgcaacggca accaagagga ctccgatcc
//
LOCUS       DASULZ010000287         5479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_339614, whole genome shotgun sequence.
ACCESSION   DASULZ010000287 DASULZ010000000
VERSION     DASULZ010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339614"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VFY96_06015"
     CDS             complement(<1..678)
                     /locus_tag="VFY96_06015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HEX5606050.1"
                     /translation="MYVAHYLNQFFGGIGAEEHAGEGLQICDSAVGSGKLLESALGND
                     VRVVVTFICGDNYAVEHQDELTTAVLAKLRTANIDLFVAGPCFDAGRYGMAAAALCRA
                     VQKELSIPVVTAMSEENPGVDLYRDALYIIDSGASVARLRDVIVKMAALSKKLLAKLP
                     IGLPDEDGYLQRGWLRDQLVEQRAATRLVDMLLAKMTGKPFRSEMPATSFAPVPIPAA
                     VKNLSKAK"
     gene            complement(735..2015)
                     /locus_tag="VFY96_06020"
     CDS             complement(735..2015)
                     /locus_tag="VFY96_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HEX5606051.1"
                     /translation="MQLTLANHPITDLQFGAPARLDGTVLIVDADSLRRVILEDSAFV
                     GVHFEIARSGEPCRAGPIFDIVEPRAKAPASGADFPGILSPATTAGLGTTHVLKGVAV
                     SVLAEISPDLTRSATGRVLELSGAPAEASDYANLHHLLVIPQTKPELSRQAIEKAYRI
                     AGVRLAVALARTACNQAPDSVHQFDAVGPAETGREGLPRVAYVGQIFSRQRKPEVDEP
                     ILYGANTDGMLPVLLHPDEWLDGAVVPSLHSWFGGTETYFYQNHPIILDLYRRHHARE
                     INFVGTIATIAGADNFDRERHCQAAANLVKWNLRSEGAVLSKYGGGLPHADLAETARL
                     LETMGIRTAVMVSDVSRDRRVESALLFNVPEVDAIVYNGGNGMRWELPRLDRVIAATP
                     QFRNLLTGPMTIDAPNVIGVTNQQGASRMRAMVY"
     gene            2194..3687
                     /locus_tag="VFY96_06025"
     CDS             2194..3687
                     /locus_tag="VFY96_06025"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HEX5606052.1"
                     /translation="MLRFSPNRNLARLVRWFESEDDAFATARVQDRPVAMFLAAFWCR
                     YCQRMDEEAFSDRENMALLNAYFVALRVENAKRPDIDTRYNLNGWPTIAFLTPNGKLL
                     AASNYLPTAEFQELLLNVYMEYEKHKEEFRSGVRSATLNPVTPKCFIRREPDVSFLKD
                     ITESIIGTADHDNGGYGNGQKFIHAEVNEFLLSHYDRTHDARLLDHVRLTLEQMRSSA
                     IYDAEEGGYFRTTTGADWVQPHREKLLSEQAGLLLNCLHLFRITESREHARIAEEIID
                     YLDHKLFDSAKPAFFGCQDFLRVETKSDEFFTIIDDCIYTDANALAVIAYLDAAAILN
                     RPEHEARALKVLDFLWHHNRDEQRGMFHYFDEAPQIPGLLIDQARMGIALVRAFVATG
                     ENSLLDRATKLADVIVAQLACPAGGYFDRGQSDLDFFGPRLILIDQNGITASFFLMFA
                     NATGDAKYRDAAVRAFSAFRGDFASYGIQAAPFGQAIGEWLRQEESE"
     gene            complement(3759..4778)
                     /locus_tag="VFY96_06030"
     CDS             complement(3759..4778)
                     /locus_tag="VFY96_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606053.1"
                     /translation="MISQSVTEKNSDELDNYKIGTGPYKLVTWQRAGNLVLARNDAYW
                     GAKPQISEVLFKTVKEEAARVAGLLAGQADVISNLPIEEIDRVQKSPRARVEKVPGYR
                     MYFLVMNVTHKPFDNKLVRQAFSYSIEPAIILKHVFEGNGYALNGPLSSNMIGYDPAM
                     RRYPSDAKKARELLARAGYPNGLDVKLYLSPDRHLKGKEVCQVIARQLSESGINVELV
                     PQEYPVYWGRDGVNGGKLAFYYAGRSAYDADTFYDQYFHTGVTKRTGYSNPELDRLIE
                     EEQQTGDYKKRLSLLQQAGRIVMEEALVVPLYTLAEIYGVAKNIIWQGNPNNEIIVAG
                     MKIKA"
     gene            complement(4760..5290)
                     /locus_tag="VFY96_06035"
     CDS             complement(4760..5290)
                     /locus_tag="VFY96_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606054.1"
                     /translation="MHVNPSDINRRQFLLNSAALSIGLINLRAPRAGIAASRDRIVVF
                     QGVGLDSLHPYAYSGGGISGIWQHVIEPLVEMDFSRKEYVGVLAESWQFQGRKWTFHL
                     RKNVRFHDGSTLTSKDVLFSIDRMTHDRKSMQASNFAEVTGIEAPDDYTVVITTKQPN
                     AVLLERLNTALSSANR"
BASE COUNT         1235 a         1503 c         1501 g         1240 t
ORIGIN      
        1 tttggccttc gataaattct ttacagccgc cgggatcggt acgggtgcaa aggaggttgc
       61 cggcatctcc gatcggaatg gcttccccgt cattttcgcc aagagcatgt cgacgagccg
      121 ggtcgccgcc ctttgctcga ccaactgatc gcgcagccag ccgcgctgca gataaccatc
      181 ttcgtcaggt aaacctatag gtagtttggc gagaagtttt ttgctgagcg ccgccatctt
      241 gacaatgacg tcacgcagcc tggcaacgct ggctccggaa tcgatgatgt aaagcgcatc
      301 gcgataaaga tcgacgccgg gattctcttc gctcatggcg gtgacgaccg gaatgctcag
      361 ttccttctgg actgcgcggc aaagagcggc ggcagccatg ccgtaacgac cggcatcgaa
      421 gcagggcccg gcaacgaaca ggtcgatatt tgcggtccgt agttttgcca aaaccgcggt
      481 cgtaagctcg tcctgatgct cgacggcata gttgtcgccg cagataaagg tcacaaccac
      541 acggacatcg ttgccgagtg ccgattccaa gagcttcccc gatccaactg cactatcaca
      601 aatctgcaag ccttcaccgg cgtgctcctc tgcgccaatc ccgccaaaaa actggttcag
      661 ataatgtgcg acatacatgt gaactcctaa tgccggataa gaagtgcgca gcatgctgcg
      721 ccccgaacga tggcctaata gaccattgcc ctcattcgcg atgcaccctg ttgattggtg
      781 acgccgatta cgttgggcgc atcaattgtc atcggtccgg tcagcaaatt ccggaactgc
      841 ggtgtcgcgg cgatcacccg gtcgaggcgc ggcaattccc agcgcatgcc gttgccgccg
      901 ttatagacga tcgcgtcgac ttcggggaca ttgaataaaa gcgccgactc cacacgccga
      961 tcgcgtgaaa catcggagac catcaccgcc gtgcgaattc ccattgtttc gagcagacga
     1021 gcggtctccg ccagatcagc atggggtaaa ccaccgccgt atttcgagag caccgcgcct
     1081 tcggaacgca aattccactt gaccagatta gccgcagcct ggcagtggcg ctcgcgatcg
     1141 aaattgtccg cgcccgcaat ggtcgcgatc gtgccgacga aattgatctc acgagcatgg
     1201 tggcggcgat aaagatcgag gatgatcgga tgattttgat aaaaataggt ttccgtgccg
     1261 ccaaaccagg aatgaagaga cgggacgacg gcgccgtcaa gccattcgtc aggatggagc
     1321 agcaccggca acatgccatc agtattggcg ccgtaaagaa tcggctcatc cacctccggc
     1381 ttgcgctggc gcgaaaagat ctgcccgaca taagcgacgc gcggcaaacc ttcacggccg
     1441 gtttccgccg ggccgaccgc atcaaattga tgcaccgaat ccggtgcctg attgcaagcg
     1501 gttcgcgcga gtgctacggc gagtcgaacg ccggcaatac ggtaagcttt ttcaatcgct
     1561 tgacgactca attctggttt ggtttgcgga atcaccagta agtggtgaag gtttgcatag
     1621 tcgctcgcct ctgccggagc accgctcaac tcgagcaccc tacccgtcgc gctgcgcgtc
     1681 aggtcgggtg aaatttccgc gagcacggag acagcgacgc cttttagaac gtgggtcgtt
     1741 ccaagtccgg cggttgtcgc ggggcttaaa attcccggga aatcagcgcc ggaggccggt
     1801 gctttggctc gcggctcgac aatatcgaag atcgggccgg cccggcatgg ctcgccggaa
     1861 cgggcaattt cgaagtgaac gccaacaaac gcgctgtctt ccagaataac tcgacgaagg
     1921 ctatccgcat caacgatcag aaccgtgccg tcgagacgcg ccggagcacc aaactgcaaa
     1981 tcagtaatcg ggtgatttgc caaagtgagc tgcattgatg tttcctcaac accgccgtga
     2041 aaagacgaat cacgacggat ggcgcgatct tatgccgtgc agacgaaagt tgtcaaagag
     2101 gagaaaagcc tcagtccggc agcaggctac cgaagcctta gagattgccg tgaaaggatg
     2161 ataatagcga gtagaggttg acgtgccatc tttatgctgc gcttttcacc aaaccgaaat
     2221 ctcgctcggc tcgtccgttg gttcgagtct gaggacgacg ccttcgcgac cgcgcgtgtg
     2281 caggaccggc cggtagcgat gtttttggcg gcgttctggt gccgttattg ccagcgtatg
     2341 gatgaagaag ctttctccga tcgagaaaac atggccttgc tgaacgcgta tttcgtcgcg
     2401 ttgcgcgtgg agaatgcgaa gcggccggat attgacactc gatataacct gaacggctgg
     2461 ccgacaatcg cgttcctaac cccgaacgga aagctactgg cggccagcaa ttatcttcct
     2521 acggcggaat tccaagagct gttactgaac gtctatatgg aatacgagaa gcacaaagaa
     2581 gagtttcgct caggcgttcg aagcgcaact cttaaccccg tcacgccaaa gtgtttcata
     2641 agacgcgaac ccgacgtgtc ctttctcaaa gacatcaccg agtcgatcat tgggacggcg
     2701 gatcacgata acggcggcta cggcaatggt cagaaattta ttcatgccga ggtaaacgag
     2761 ttcttgctgt cgcactacga tcgaactcat gacgcgaggc tcctcgacca tgttcgtttg
     2821 acattggagc aaatgcgttc gagcgcgatc tatgacgccg aagaaggtgg atatttccgc
     2881 accaccaccg gcgccgactg ggtgcaaccc catcgcgaaa agctgctcag cgagcaagcg
     2941 gggttgctgc tgaactgcct gcacctgttt cgtattaccg aaagccgtga gcatgcgcgg
     3001 atcgccgagg aaatcatcga ctacctggat cacaagttgt tcgattcagc gaaacccgcg
     3061 ttttttggct gccaggattt tctgcgcgtc gaaacgaaat ctgacgagtt ttttacgatc
     3121 atcgatgatt gcatctacac cgatgcgaac gccctcgctg tcatcgctta tctggacgcc
     3181 gcggctatcc taaatcggcc cgagcacgaa gcccgggctt taaaggttct cgactttctt
     3241 tggcatcata accgtgacga acaacgaggc atgttccatt acttcgatga agcgccacag
     3301 attcccggtc tgctcatcga tcaggcccgg atgggaatcg cgctggtgcg cgcatttgtt
     3361 gccacgggcg agaatagtct tcttgatcgc gccacgaaac tggcggacgt gatcgttgcg
     3421 caactcgcat gtccggcggg cggttacttc gatcgtggtc agagtgactt agattttttt
     3481 ggaccgcgtt tgattctgat cgatcaaaac ggcatcacag catcgttttt tctgatgttt
     3541 gcgaatgcta ccggagacgc gaaatatcgg gatgcagccg ttagggcatt cagtgctttc
     3601 cgcggcgatt tcgccagcta tggaattcag gcggcgccct ttggtcaagc cattggagaa
     3661 tggctgagac aagaagaatc ggaatgatgg agtgttggag tattggagaa tcagaaaacc
     3721 caacccgcaa tttctatcac tccagcactc caaccgattt acgccttgat cttcatgccg
     3781 gcaacgataa tttcattatt cggattgccc tgccagataa tgtttttcgc tacgccgtag
     3841 atctcagcga gggtatagag cggcaccacc aacgcttctt ccatcacgat gcggccagct
     3901 tgctgtaaga gactgagacg ttttttgtaa tctccggttt gctgttcttc ctcgatcagc
     3961 cgatccaact cgggattgct gtagccggtg cgtttggtca cgccagtgtg aaagtattga
     4021 tcgtaaaaag tgtcggcatc gtaagcggat cggccggcat agtagaacgc gagcttgccg
     4081 ccattcacgc cgtctcgtcc ccagtaaact ggatattcct gcggcaccaa ttcgacgttg
     4141 ataccgcttt cggaaagctg gcgcgcgatg acttggcaaa cctctttgcc cttgagatgg
     4201 cgatcgggcg agaggtaaag cttgacgtcc aaaccgttcg gataacctgc cctagccaga
     4261 agctcgcgcg ctttttttgc atcggaggga tagcgcctca tggccggatc atagccaatc
     4321 atatttgagc tcaatgggcc gtttagcgca taaccatttc cttcgaaaac gtgcttcaga
     4381 ataatcgccg gttcgatcga atagctaaac gcttgccgca cgagcttgtt atcgaacggc
     4441 ttatgcgtaa cgttcatgac caaaaaatac attcgataac ccggaacttt ctccacccgc
     4501 gcgcgcggac ttttttgcac ccgatcgatt tcttcaatcg gcagattact gatgacatcg
     4561 gcctgaccgg cgagtaagcc ggcgacgcgc gccgcctctt ctttgaccgt tttgaatagg
     4621 acttcgctga tctgcggttt cgcgccccag tatgcgtcgt tgcgcgcgag caccaaattt
     4681 cctgcgcgct gccaggtgac cagcttgtaa ggcccggtgc cgattttgta attgtcgagc
     4741 tcgtcagaat ttttttcggt taccgattgg ctgatgataa agcggtattc aaccgttcga
     4801 gcaagaccgc gtttggctgt ttggtcgtga tgaccacggt gtaatcgtcc ggagcttcga
     4861 tcccagtcac ctctgcgaaa ttggacgctt gcatgctctt ccgatcatgc gtcatgcggt
     4921 cgatcgaaaa tagaacatcc ttggatgtca acgttgaccc gtcgtggaaa cggacgttct
     4981 ttctcaagtg gaaagtccat ttccggcctt gaaattgcca cgactcggcg agcacgccga
     5041 catattcctt gcgggaaaaa tccatttcga ccagcggctc gatgacgtgc tgccaaatac
     5101 cgctgatgcc gccgccgctg tatgcgtagg gatgaagcga atccaaaccg acgccttgaa
     5161 aaacaactat tcgatcgcgc gaggcggcaa tgccggcacg aggcgccctg aggttgatga
     5221 ggccaatgct gagggcggcg ctgtttagca ggaattgccg acggttaata tcgcttgggt
     5281 tcacgtgcat tttttctccg gtgaaactgc acaagagccg gcacagaaat gcgcagtgga
     5341 ctgagtcgaa gttgttgacc accatcgctt atagctgtgt gattcgaatt gcgtcaagtg
     5401 gattcgcgct cgccgtatcc ggcctctgtt atgcatttgc gatctggttg cgcctagcgt
     5461 gcgagagcca tgacccttg
//
LOCUS       DASULZ010000288         3289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_644503, whole genome shotgun sequence.
ACCESSION   DASULZ010000288 DASULZ010000000
VERSION     DASULZ010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644503"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..1292)
                     /locus_tag="VFY96_06040"
     CDS             complement(198..1292)
                     /locus_tag="VFY96_06040"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5606055.1"
                     /translation="MAFNGHTVIDGDGHVIEDCQAIINYMPQPYRDKYETHSFFNPFP
                     PLDHLHSANLHDFQPGAFNKVGPEGWVDFLEDVGIETTVLYTTLGLAFGKIVSRDWAI
                     DVARAYNDWIHNTYMKKNPRFKAMGLIPLQEPSAAVEELRRIVNELGMCGAMLPSTGI
                     QANLGDQRYWPIYEEANRLGCAIGIHGGAHESLGLDDLSPYAPVHSLGHPFGQMISFG
                     GLVFNGVFDKYPNVKFGFMEGGVAWLLVCLERFDRSWETHIQHDPRKRFLELRPKEKV
                     SDYIARHIDGSRIFVGCEGEEPDIAHAIKRVGNKPWVYSSDYPHEVNNEFCKHEIYEF
                     LENEEITAADKAAFLHGNARRFYSLGAPGL"
     gene            complement(1412..2485)
                     /locus_tag="VFY96_06045"
     CDS             complement(1412..2485)
                     /locus_tag="VFY96_06045"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX5606056.1"
                     /translation="MRRFAVVLFLLCFAPYTLGQSQPSRLQVATRLVRPFVYEQQGQL
                     TGFSIELWQEIARQLNIQSEFVVKATVKELLDTVKSKNTDAGIAAISITADREIDFDF
                     SQPMFDSGLQILAPVQGSRTALVEAIVGNLFSTTVLLYVLVIALILFLIAHLVWWFER
                     GNPTGMLTQRSYFPGIFEACWWAASTLATQADQMPRAAIARIIAVVWMFMSVVFIAYF
                     TASVTSSLTVQQLRGEINGPEDLPGKRVGSVHGSTSAEYLRQRNIEPIEFASIEEGYQ
                     ALEDHQIDAVVYDTPILLYYTAHEGKGKAQTVGAMFRKENYGIVFPSNSPLRKRVNEA
                     LLKLKENGAYDQLYTKWFGGTAS"
     gene            complement(2504..3175)
                     /locus_tag="VFY96_06050"
     CDS             complement(2504..3175)
                     /locus_tag="VFY96_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606057.1"
                     /translation="MTLAIYKHPGLVVALVLSGLVCLVAPRGIAGAQTRRNIKVVLES
                     RQSGTSNRTGAQGSGSIVVRRGTVYPSGRIAAGNQQTTVERSSGIFTIVLDGGESLLT
                     VATQVPQSEAAYYFNYALGLGYIERHIVFNDVGTSLRVGAAILSDGQIRLRLTPRISY
                     FSIDRPGAIDCTEAATDLIVRNGEAIPVGGSTSKLHEVTRQILGYSSRSSTDETDLTV
                     TATVQ"
BASE COUNT          724 a          896 c          922 g          747 t
ORIGIN      
        1 cgtaggtgtt aaactgcggc tcggtcaggc gattgctcga tgggatgatc aagccgatgc
       61 gcggttcggg agtcatttct gtccaaagtc gttcgaaaag ttcaaatcgt tcaatcgttc
      121 gcgttgctcg ctccgttcaa ccgcttcgct cgaacggttt gaacgggttc aacctcttga
      181 acggcttgaa ccatcggtta cagcccgggc gctccgaggc tataaaaacg ccgcgcattg
      241 ccgtgcagaa acgccgcttt atcggccgcg gtgatttcct cgttctcgag aaattcatag
      301 atctcatgtt tgcaaaattc gttattcacc tcgtgcggat agtcgctcga ataaacccag
      361 ggcttgttgc cgacccgttt tatcgcgtga gcgatatccg gctcttcacc ttcacagccg
      421 acgaagatgc ggctgccatc gatgtgccgc gcgatgtaat cgctgacttt ctccttgggc
      481 cgaagctcta aaaagcgctt acgcggatca tgctggatgt gcgtctccca ggagcggtcg
      541 aagcgctcca agcacacgag aagccaggca acgccacctt ccataaagcc gaatttcacg
      601 ttcgggtact tgtcaaaaac gccgttgaag acaaggccgc cgaaggagat catctggcca
      661 aaaggatggc ctaacgagtg gacgggtgca tacgggctta aatcatccag gcccaagctt
      721 tcatgagcgc cgccgtgaat cccgatggca cagccgagcc ggttcgcttc ctcgtaaatc
      781 ggccagtacc gctgatcgcc tagattagcc tggataccgg tggagggcag cattgcgccg
      841 cacatgccca gttcattgac gatcctgcgc aactcctcga cagcggctga tggttcctgg
      901 agcgggatga ggcccatcgc cttgaagcgc ggatttttct tcatgtaggt attgtgaatc
      961 caatcgttgt aggcgcgtgc gacatcgatc gcccagtcgc ggctaacgat ttttccaaac
     1021 gccaagccca gggtggtgta aagcaccgtc gtttcgatgc ctacgtcctc caagaaatca
     1081 acccaacctt cagggcctac cttgttgaac gcgcccggct gaaagtcgtg cagattggcg
     1141 gagtggagat gatcgagggg cggaaacgga ttgaaaaagc tgtgggtctc atacttatca
     1201 cgatagggtt gaggcatgta attgatgatc gcctggcaat cttcgatcac gtgaccgtcg
     1261 ccatcaatga ctgtgtggcc gttaaacgcc atgggcattc cctcctcagg ataggtcatc
     1321 aagcaggacc atttttgtca agtccgaaga atcccgcttt gtagtttctc gttgttccta
     1381 tatctggcga tcagagaccc ggacctagta actaactcgc agttccgccg aaccatttcg
     1441 tatataactg atcgtaggcg ccgttttctt tcagcttcag cagcgcttcg ttgacccgct
     1501 ttcgtagcgg gctattggac ggaaatacaa tgccgtagtt ttctttgcga aacattgcac
     1561 ctacggtttg tgctttgcct ttgccttcgt gggccgtgta atagaggaga atcggcgtgt
     1621 catagacgac ggcatcgatt tgatgatcct cgagggcttg ataaccttct tcaatgctcg
     1681 cgaattcgat cggctcgatg ttgcgttggc gcagatattc cgcggaagta ctgccgtgga
     1741 cgctgcccac ccgtttcccc ggcagatcct cgggaccgtt aatctcgccg cgcaactgct
     1801 gcacggtcaa tgacgaagtg acggacgccg taaagtaggc gatgaaaacc acgctcatga
     1861 acatccagac cacggcgatg atgcgcgcta tggccgcgcg cggcatctga tccgcctgag
     1921 tggcgagggt ggaagcggcc caccagcaag cctcgaaaat tcccggaaag taggatcgct
     1981 gggtcaacat gccagtcggg ttgccccgct caaaccacca aaccaagtgg gcgatgagaa
     2041 aaagaatcag cgcgatcact agaacataga gcagcaccgt cgtggagaat agatttccga
     2101 cgatcgcttc gactaacgcc gtgcggcttc cctgcaccgg tgcgaggatc tgcaacccgg
     2161 aatcgaacat cggttgagaa aaatcgaaat cgatttcgcg gtccgccgtg atcgagatcg
     2221 cggcgatacc ggcatcggta tttttcgatt ttaccgtgtc cagcaattct ttaacggttg
     2281 ctttaacgac gaattccgac tgaatattca gttgcctggc gatctcctgc cagagctcaa
     2341 tgctgaagcc cgtgagttgt ccctgctgct cataaacgaa tggtcggacc aaacgcgtgg
     2401 cgacctgcaa tctgctcggc tgggattggc cgagcgtgta cggcgcgaaa catagtaaga
     2461 agagaacgac agcgaatcgg cgcattttgc tacgagctgt aaattactgg acggtggctg
     2521 tcacggtgag gtcagtttcg tcggtgctcg agcggctgga gtagccgaga atttgccgag
     2581 tgacctcgtg tagcttactc gtggacccac cgaccggaat cgcttcgcca ttacgcacga
     2641 tcaagtccgt cgccgcttcg gtgcaatcga tcgcgcccgg tcggtcgatg gaaaaatagc
     2701 tgatgcgcgg cgtcaggcgc agcctgattt gaccatcgct cagaattgca gcgccgacgc
     2761 ggagcgatgt tccgacgtcg ttaaacacga tgtgccgctc gatgtagccg aggccgaggg
     2821 cgtagttgaa gtaataagcc gcctcgctct ggggcacctg ggttgcaacg gtaagtagcg
     2881 attcgccccc atccaagacg atagtaaaaa ttccgctgga acgttctacc gtcgtctgct
     2941 ggttgcccgc ggcgattctg ccggaaggat agaccgtccc tcttcggacg acgatgctgc
     3001 cgctgccttg ggcgcctgtg cggttgctgg tgcctgattg gcggctttcg agaacgacct
     3061 tgatattgcg gcgagtctgt gcacccgcga ttcccctcgg ggctacaaga caaaccaaac
     3121 ctgataagac aagcgcaacg acaagtccgg ggtgcttata aatggccagc gtcattgtcg
     3181 gcttgcgggc agcaatgaaa agtcgacgac cttttgcaga tcaaagtctg ctgggatcgg
     3241 ccgttcctga cgcagaatat ctaaatagcc gcgtgcgact ttgggattg
//
LOCUS       DASULZ010000289         2114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1455821, whole genome shotgun sequence.
ACCESSION   DASULZ010000289 DASULZ010000000
VERSION     DASULZ010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455821"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="VFY96_06055"
     CDS             <1..1020
                     /locus_tag="VFY96_06055"
                     /inference="COORDINATES: protein motif:HMM:NF039581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX5606058.1"
                     /translation="QSAGASPGPACYGQGGTEPTVTDANLIAGRIDPEFFLGSGIRLQ
                     REKAAQAILEKIGKPLGLSLEEAALGILTIANFNMSLSVRAVSVEKGYDPRDCTLVPS
                     GGGGALHAMAIARELSVPRVIIPPMPAHFSALGMLMADLKHDYVQTFVRELAETSGSQ
                     IADAFALLEKSAIETLTEEGSKPEQIVLRHFLDMRYRGQEYTLPVPVTEDLRGLSDFG
                     AIRARFDQLHQEHYGHSASNEPVMMVNLRLSALGKFDNKLPLSSTARDADRGERGRRR
                     VIFESSPVVDCPIYLRTGFRAGDRLEGPAVIEEVGATILLYPGDRMQVNEFGHLVIEV
                     TAGSS"
     gene            1151..>2114
                     /locus_tag="VFY96_06060"
     CDS             1151..>2114
                     /locus_tag="VFY96_06060"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX5606059.1"
                     /translation="MADQTLSETLAGYIASLSSETLPADIIESAKLHILDSLGCLLAG
                     SRLHAGKLAYDLAVANSGANANSRLLGSQRRSSLLDGVEAMSVAAHCGEMDDIHSGAG
                     TCIGALVIPALLALAETYGGSGGNFIEATIAGYETTIRVGLSIDGPKLFARGWWPSTI
                     CGAFGVAAAGAKFLKWTGRETANALGIAALHSGGMLTGGNEGATARHLAFGHAARNGI
                     QSLLAAQQGFTGPTQAFEDARGFCSTLCSEPKWEHLRSFDGFHLPEVAFKPYPCARQL
                     HAGVEALLRIIRQHPLQPELIRALELFLPGQNAAMMNRPSITPTH"
BASE COUNT          436 a          600 c          644 g          434 t
ORIGIN      
        1 caaagcgccg gcgcttcgcc cgggccggcc tgctacggtc aaggaggaac cgaaccgacg
       61 gtgaccgacg ccaatctcat cgcaggccgc atcgatccgg aattttttct tggcagcggc
      121 atccgcttgc agcgcgagaa agcggcgcaa gcgattctgg aaaaaatcgg taaaccgctc
      181 ggtctgtcat tggaagaagc ggcgttgggg attctcacca tcgccaattt caacatgtcg
      241 ctttcggtgc gcgccgtctc ggtggaaaag ggttacgatc cccgcgattg cactctggtg
      301 ccgagcggtg gtggcggcgc gttgcacgcc atggcgattg cgcgggaatt atccgtgcca
      361 cgcgtcatca tcccaccgat gccggcgcat ttttctgcgc tcgggatgct gatggcggac
      421 ttgaagcacg attatgtgca aaccttcgtg cgtgaattgg cggaaacgag cggctctcag
      481 atcgctgacg cattcgcgct tttggaaaag tccgccattg agacgctcac agaggaaggc
      541 agtaagcctg agcaaatcgt gttgcgtcat ttcctggaca tgcgttatcg cgggcaggaa
      601 tacacattgc cggttcccgt gaccgaagat ctacgcgggc tgagcgactt cggagcgatt
      661 cgcgcgcgct tcgatcagtt gcatcaagag cactacggcc actcggcttc gaatgagccc
      721 gtgatgatgg tcaatctgcg cctttcagcg ctggggaaat tcgacaacaa actgccgctg
      781 agctcaacgg cgcgtgatgc ggaccgcggc gagcgcggac ggcgccgggt gattttcgag
      841 agctcacccg tcgtggattg cccaatttat ttacggactg gatttcgagc cggcgaccgg
      901 ctcgaaggac ctgcagtcat cgaggaagtc ggcgcaacaa ttcttctcta tcccggcgat
      961 cgcatgcagg tgaatgagtt cggccatctg gtgattgagg tgacggcggg ttcctcatga
     1021 cggaaatttg ctcaagttct ctcacggact ccagcccgaa tcttaccgag gcttcgcgat
     1081 gacagaaaaa cggccgtcat cctgagcgca agcgaaggat ctcaacgaca cagcgactcc
     1141 cgtcgattgc atggctgacc aaacgctgag cgaaacgctc gcaggataca ttgcatcact
     1201 ttcgtccgag acgctgcccg ccgatattat cgagtccgcc aagctgcaca tactcgattc
     1261 ccttggatgc ctgctcgcgg gctcgcgtct tcatgcgggc aagcttgcgt atgatctagc
     1321 cgttgcgaac agcggagcaa acgccaattc gagactttta ggatctcaac ggcggagttc
     1381 tttgctcgat ggtgtcgaag cgatgtccgt tgccgcgcac tgcggcgaga tggacgatat
     1441 tcatagtggc gcgggaacat gcattggcgc gctggtgata ccagcactgc tcgcattggc
     1501 ggaaacatac ggcggcagcg gtggaaactt tatcgaagcg acgattgccg gctatgaaac
     1561 gaccattcga gtcggcctca gcatcgatgg gccaaaactg tttgcgcgcg gttggtggcc
     1621 gagcaccatc tgtggcgcgt tcggtgtggc tgcggcgggc gcgaagtttc tgaagtggac
     1681 cgggcgagaa accgccaatg cgcttggtat cgcagcactg cacagcggtg gcatgttgac
     1741 cggcggcaac gaaggcgcga ccgcgcgaca tctcgcgttc ggccatgcgg cgcgaaacgg
     1801 tatacaatca ttgctggcgg cgcagcaggg attcaccgga ccgacgcagg cgttcgaaga
     1861 tgcgcgcggt ttctgttcga ccttatgcag cgagccgaaa tgggaacatc ttcgaagctt
     1921 tgatggcttt catcttccgg aggtcgcatt caaaccctat ccgtgcgcgc gccagctgca
     1981 cgccggagtc gaagcgctgc tcagaatcat tcgacagcac ccgttacagc ctgaactcat
     2041 ccgtgcgctc gagctttttc tgccgggaca gaacgcggcg atgatgaatc gtccatcgat
     2101 cacgccgacg cacg
//
LOCUS       DASULZ010000290         5800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_36606, whole genome shotgun sequence.
ACCESSION   DASULZ010000290 DASULZ010000000
VERSION     DASULZ010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5800
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36606"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VFY96_06065"
     CDS             <1..635
                     /locus_tag="VFY96_06065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5606060.1"
                     /translation="FRGHGNLSRMNRSNENKEMVADGISDAECVRRVQRGETDAFETL
                     IRRHEKTIFNLVYRMLGDTEEAAEVSQEVFLSAFRAAGQFRGDANFSTWLYRIAINHA
                     STRRKTLNSRQRKVVPIESADSLHDPEPGPLEILEQQEIREKVQFALNQLEPEDAAVI
                     LLRDLQDVPYNEVAHVLEIPVGTVKSRLHRARQALKSRLQSYFCAERKAV"
     gene            632..1771
                     /locus_tag="VFY96_06070"
     CDS             632..1771
                     /locus_tag="VFY96_06070"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX5606061.1"
                     /translation="MTCAEVQRLLSEYIEKSLDTIRMKAVETHLLSCSGCRAATEGLM
                     DCIRQISELPLLDPPIGFTQRVMAHVREIEARPSRWRRVLAPFRVSAPLQATAVVLIA
                     VLAVFLYQKEPQIRDNPVAPAIPSILPSQPLSDHGSDDHSGGKPDQPTASTQRRADIP
                     RQASSTANAVRSSTTESRKKASAATVTDQVKPKTPSSPHPEANSGGDIKNPLSRRGPI
                     QAQEIATGMEGLRSSSDSFGFGPTIGGSLRPGFFLPERSLSPITEPSADAEFVVRRRE
                     AQMRDQNSIARSESARQSAESDVAPAVAKQSSATSSSPSSSINLREIRWFSVPADQYD
                     QFRKDLASEASIDSEKTIGVSERDFALKSNRELLIKVIIVSPAER"
     gene            complement(1923..2900)
                     /locus_tag="VFY96_06075"
     CDS             complement(1923..2900)
                     /locus_tag="VFY96_06075"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5606062.1"
                     /translation="METVVARSARDQATVSRQPRFSGLSHVSLACRDLVESKLFHSAV
                     FGGELVHEIRGFVEYRIADVIFGLAEQNQGWTAPDHEYPHYAFYLDGANFERMIHWLD
                     LYNVPNYPYRRDATALLYFRDPSGNLFELYCDTGYERIASLPAAPRRGGAPIDFRSLN
                     YHWSGNRAADSSGTQKPQFSGFAHASLYCRDLAQAKRFFTNVMSGELIHDVDSFAEAR
                     VAGVIIGITIRPGTTTSRDAEYPHYAFFVEPKDFLPMLAWLRQNGVNTSEPWTRDGVK
                     GLVYFRDPAGNLFEMYCPKLSEAASFVRGKKQGGSYEIDFAALNYEWQG"
     gene            complement(2917..3393)
                     /locus_tag="VFY96_06080"
     CDS             complement(2917..3393)
                     /locus_tag="VFY96_06080"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX5606063.1"
                     /translation="MRSTVRFILAVLTNRRTLRIEWGQCDPAGIIFYPQYLIIFDGST
                     GWLFERTGLTPSRMRQRYNIVGMPIVEVGGRFLKPCRFDDEIVVESEVSEWGRSSFTV
                     RHRIFNQTDLAVDGFEKRVWAAADPERPGAIKGQAVPEEIIASLSDPTGATIIKAR"
     gene            complement(3460..4176)
                     /locus_tag="VFY96_06085"
     CDS             complement(3460..4176)
                     /locus_tag="VFY96_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX5606064.1"
                     /translation="MQIDNDDIGGVVTSGKGPEAGVWVIAETSDLPTRFARTVVTDHL
                     GRYVIPDLPKVNYDVWVRGYGLVDSPKVKSVPGKILNLEAVIAPTESAAARYYPAIYW
                     YSMLKIPDKSEFGGNGRIAANITQNEWLTMMKSRSCIGCHQLGQLSTRTFPQNLPHPI
                     KEATSEEAWFRRIQSGQAGEWMFDALVKQLGGAPLIYLADWTDRIAKGELPHTKPARP
                     QGIERNVVVHFQLRPDPLAN"
     gene            complement(4928..>5800)
                     /locus_tag="VFY96_06090"
     CDS             complement(4928..>5800)
                     /locus_tag="VFY96_06090"
                     /inference="COORDINATES: protein motif:HMM:NF014489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxyl carrier protein"
                     /protein_id="HEX5606065.1"
                     /translation="HGHCNNGLGPFNYLEAMKLGIETLHTAIPPLANGSSQPSIFNIA
                     ANARALGFEPVVDLEAVKPVEQHFTYIAKREGHPIGAQREYDHAQYLHQVPGGMISNL
                     RHQLRTMGMENRLQETLQEAGRVRAEFGYPIMVTPLAQFVGTQAAVNVITGERYKEVT
                     DQSIQYALGHWGKEAPAVMDPNVKDKILNRSRANDWRNWSPPDLSLKEVRQKFGGPGV
                     SDEELVLRVIAGVNAVRTMLAAGAPREYVSATQPLVKLIGELTKRRDCSQVYIQKGDF
                     SLRLEKNQSGRYVH"
BASE COUNT         1306 a         1696 c         1538 g         1260 t
ORIGIN      
        1 tttttcgcgg ccatgggaac ctttcccgaa tgaatcggtc taatgagaat aaagaaatgg
       61 tggcggacgg catcagtgac gcggaatgcg tccggcgggt tcagcgcgga gaaaccgacg
      121 cctttgagac tttgattcgc cgtcacgaaa aaacgatttt caatttggtc taccggatgc
      181 ttggcgatac agaggaagct gcggaagtct cacaagaagt gttcctgtcg gcgtttcgcg
      241 cagccggcca gtttcgcggt gatgccaact tctcgacttg gctgtaccgc atcgccatca
      301 accatgcgag cacgcgccgg aaaacgctta acagccgcca acgaaaagtg gtgccgatcg
      361 agagcgcaga ctcgcttcat gatcctgagc cgggccctct ggaaatactc gagcaacagg
      421 agatccgcga aaaagtgcag ttcgcattga accaattaga gcccgaggac gctgcggtca
      481 tactgctcag ggacctgcaa gacgtcccct ataacgaagt tgcccatgta ctcgaaatcc
      541 ccgtgggcac ggtgaaatct cggctacacc gcgcgcgcca agctttgaaa tcccggctcc
      601 aatcctattt ctgcgcggag agaaaagctg tatgacgtgc gcggaagtac aacgactgct
      661 gtccgaatat atcgaaaaat cgctcgacac gattcgcatg aaagcggtcg agacccattt
      721 gctctcatgc tctggctgcc gcgctgcgac cgaaggcttg atggattgca tccggcaaat
      781 ctccgagctg ccgctgctgg acccgccgat tggcttcacg cagcgagtca tggcgcacgt
      841 gagggagatt gaagcgaggc cgagccggtg gcgaagagta cttgcgccgt tccgagtgag
      901 cgcgccgctc caggccacgg cagtggtctt gattgccgtt ctggcggttt ttctctacca
      961 aaaagagccg caaatccgag ataacccagt ggctccggca atcccgtcga ttttgccgtc
     1021 gcaaccattg tccgatcacg gctccgatga ccattccggc ggcaaacccg accaaccgac
     1081 cgcatcaacg cagcgccgtg ccgatattcc caggcaggcg agttctacag ccaatgcggt
     1141 ccgatcatca accacggaat cccgcaaaaa agcgtcagca gctacggtca cagatcaagt
     1201 caaaccgaaa acgccatcat cccctcatcc cgaagcaaac agcggtggcg atattaaaaa
     1261 tccgctttcc cggcgcggtc cgatccaagc ccaggaaatc gccaccggaa tggaaggttt
     1321 acgctccagc agtgacagct ttggatttgg gccgacgatt ggcggttcgc ttcgcccggg
     1381 cttttttctt cctgaacgat cgctatcgcc gattaccgag cccagcgcag atgcggaatt
     1441 cgtcgtgaga cgacgagaag cgcagatgcg ggatcaaaat agcattgcgc gaagtgagtc
     1501 cgcgcgccag agcgccgaaa gtgatgtcgc gccggcggtt gccaaacaga gctcggcaac
     1561 ctcgtcttcc ccaagctcgt ccatcaatct ccgcgagatt cgttggttct cggtccccgc
     1621 tgatcagtac gaccagttca ggaaagatct cgcctcggaa gcgagcatcg attcggaaaa
     1681 gactatcggc gtgagcgaaa gagacttcgc gctgaaatcg aatcgagagc tgctgatcaa
     1741 agtcattatt gtctcgccgg cagagcgata atcaattcat catgtcacag gcgcttatcg
     1801 cggcgcttgt taaaatcagt gaggcacatt cctgtcattc taaggctcag cgaagaatct
     1861 cgttgcggca aataacactt agcacactcg ccgcaatgac cactgtaccg gcgctctccg
     1921 ctctatccct gccattcata attcaaagca gcgaaatcga tctcgtagct gccgccctgt
     1981 ttttttccac gcacaaatga cgccgcttca gagagcttcg ggcaatacat ctcgaataga
     2041 tttcccgccg gatcgcgaaa atatacaagc cctttcaccc catcgcgcgt ccagggctcg
     2101 ctcgtattga cgccgttctg acgcagccac gcgagcatcg gcagaaaatc tttcggttcg
     2161 acaaaaaacg cataatgggg gtattcagcg tcgcggctgg tcgtcgtacc agggcgaatg
     2221 gtgatcccga tgatcacgcc ggccactcgg gcttcggcga agctgtccac atcgtggatc
     2281 aactcgccgc tcatcacatt cgtgaaaaag cgcttggcct gcgcgagatc gcgacagtaa
     2341 aggctggcat gtgcgaagcc actgaattgt ggcttttgcg ttccgctgga atcggcggcc
     2401 cgattcccgc tccaatgata attgagactg cgaaaatcga tgggcgctcc gccgcgtcgc
     2461 ggcgccgctg gcaaagatgc gattcgttcg tagccagtgt cacaatacag ctcgaacaga
     2521 ttgccggaag gatcacgaaa atagagcagc gccgtggcat cgcggcggta tgggtaattg
     2581 ggaacgttgt acaaatcgag ccaatggatc atgcgctcga aattcgcccc gtcgagatag
     2641 aaggcatagt gtgggtattc gtggtccgga gcagtccaac cctggttttg ctcggcgaga
     2701 ccgaagatca catccgcaat ccggtactcg acgaaacctc ggatctcgtg caccagctcg
     2761 ccgccgaaaa ctgcggaatg gaacagcttc gattccacga gatcgcggca tgctaagctc
     2821 acgtggctca ggccagaaaa gcgcggttgc ctggaaacag tcgcttgatc tctcgcgcta
     2881 cgcgcaacta ccgtttccat agaattactc tccgtctcaa cgagctttga tgatcgtcgc
     2941 tccggtggga tctgaaaggc ttgcgataat ctcctccggc accgcttgtc ctttgatcgc
     3001 gcccggccgc tcgggatctg ccgccgccca aactctcttc tcgaaaccat caacagccag
     3061 gtcagtttgg ttaaagattc gatgacgcac cgtgaagctg ctgcgtcccc attcgctgac
     3121 ttcgctttcc acgacgatct cgtcatcgaa acggcagggc ttgagaaatc ggccacccac
     3181 ctcgacgatc ggcataccca cgatgttgta tctttgccgc atccgcgaag gcgtcaaacc
     3241 agtgcgctcg aacaaccaac cggtgctgcc gtcgaagatg atcaagtact gcggataaaa
     3301 gatgatcccc gcaggatcgc actgccccca ttcgatcctc agcgtgcggc gattggtcag
     3361 cacggccagt atgaagcgaa ctgtagagcg caagctgtgt ttattcgcat ctggaattcg
     3421 ttgcgctggc gactgatatc gttgttcatc tgctcgcgcc tagttggcca gtggatcggg
     3481 gcgaagctgg aaatgcacga cgacgttgcg ctcaattccc tgcggccgtg ccggctttgt
     3541 gtgcggcaac tcgcctttgg cgatgcgatc ggtccagtcg gcaagatata tcaagggcgc
     3601 acccccgagc tgcttaacga gcgcgtcaaa catccattcg cccgcttgtc ctgactgaat
     3661 gcgccggaac cacgcctctt cggaggtcgc ctccttgatg ggatgcggta aattctgcgg
     3721 aaacgtgcgc gtggaaagct gacccagctg gtggcatccg atgcagctcc tactcttcat
     3781 catcgtgagc cattcatttt gagttatatt cgccgcaata cggccattgc caccaaattc
     3841 gctcttatcg ggaattttga gcatcgaata ccagtaaatc gccggatagt atcgcgccgc
     3901 ggcgctctcg gtcggcgcga tgacagcctc cagattgaga attttacccg ggacgctttt
     3961 caccttcggc gaatctacga ggccataccc gcgcacccag acatcgtaat tgaccttggg
     4021 taaatcaggg attacgtagc ggccaagatg atcggtcacg acggtgcgtg cgaaccgcgt
     4081 tggcagatcg ctagtttcgg cgatcaccca aacacctgct tcgggcccct tcccgctggt
     4141 taccacgcca ccaatgtcgt cgttatcaat ttgcacggca ccgctttgtt ggctgctgag
     4201 tcgccccgga aaaatgataa tcaacgcgcc aactagacat gccgctataa ccccgcaaag
     4261 cccaattgtc ttgtttatgt ctcgcttttt tcgaacatcc atcatcacca atcctctcgg
     4321 caactaacca gcaactattt acaggcgcat atatacacca cactcccttc ggatcaaaag
     4381 ggtcgttccc aaaactacga cggccacgca atgggaactg ccattcctgg acctgccccg
     4441 gcggacggga acagcgaggt ttggatcagc tctatatgga gtagtcgcgt acacgcgctt
     4501 agatcccaaa gaccagccag ttcactgatt atctgctgcc ccgcgaaacc aacatccgcc
     4561 gcgtcttcat cgacaacaac acctcgccgg tgacgttttg ggtcgggagc aaccacggcg
     4621 cgtccgtgct gaagttggag acgttagact aaggtaacga cgttcagtaa ctcaaaggcc
     4681 ccactcgcaa gagctcgggc cttttattta tatgtcgacg gcgcttcacc atttgcgatt
     4741 tgaacatccc cctctttccc cctttgtcaa agggagataa aagaggattt ggagactaca
     4801 aatccatacc gaggcaagat ggacgggcat gcgagacaaa cattcgcgcg ctgtggggaa
     4861 ggcccagttc ggagcaacct ggatcccgct taaacatgcg ggaaatgacg gagctgcaat
     4921 tgctgattta atgaacgtag cggcccgatt ggtttttttc taaccgcaaa ctgaaatcgc
     4981 ccttttgaat gtaaacttga ctgcaatccc ggcgcttcgt tagctcgccg attaatttca
     5041 ctaacggttg tgtcgcgctc acatattccc ttggcgctcc agcagcgagc atcgttctga
     5101 ccgcgttcac gccggcgatc actcgcagga ccaactcttc atccgatact cccgggccgc
     5161 cgaatttttg ccgcacttct ttcaaagaaa ggtccggcgg cgaccaattg cgccaatcgt
     5221 tagctctgct ccgattgaga atcttgtctt tgacgttcgg atccatcacc gccggcgctt
     5281 ctttacccca atgaccaagc gcgtattgaa tcgattgatc ggttacttct ttgtagcgtt
     5341 cgccggtaat gacgttgacc gccgcctggg tcccgacgaa ctgggctagc ggcgtcacca
     5401 tgatcggata gccgaattcc gcgcgcacgc gccctgcttc ttgcaaagtt tcctgcagcc
     5461 ggttttccat gcccatggtg cgcaactgat ggcgcaagtt ggagatcatt ccgccaggaa
     5521 cttgatgcag atactgcgca tgatcgtact cccgctgcgc tccgatggga tgaccttcgc
     5581 gcttggcaat gtaagtgaaa tgctgttcca ccggcttgac cgcctcgagg tccaccaccg
     5641 gctcgaatcc aagcgcgcgg gcattggcgg cgatgttgaa gattgacggc tgtgacgagc
     5701 cgttggccag tgggggaatt gctgtgtgca gcgtttcgat gccgagtttc atcgcctcga
     5761 gatagttgaa tggtcccaag ccgttgttgc agtggccgtg
//
LOCUS       DASULZ010000291         3080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1152859, whole genome shotgun sequence.
ACCESSION   DASULZ010000291 DASULZ010000000
VERSION     DASULZ010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152859"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2341)
                     /locus_tag="VFY96_06095"
     CDS             complement(<1..2341)
                     /locus_tag="VFY96_06095"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HEX5606066.1"
                     /translation="MNSLKQLAEQGQSIWLDYIRRDLIRSGELKRLVEEDGLRGVTSN
                     PTIFDKAISGSTDYDMSLRALLADNLHAPAGDLYERLAIEDIQMAADVLRAVYDETHG
                     DDGYVSLEVSPHLADDTERTISEAQRLRTAVDRPNVMIKVPATPAGLPAIEELIANGV
                     NVNVTLMFSMHHYEAVARAYIQGLERCSNPDGVASVASFFVSRVDTAVDKALEESSNP
                     EAKALAGKIAIANSKVVYRRFLEIFHGEGFAALRQRGARVQRPLWASTGTKNPAYSDV
                     LYVENLIGPETVNTMPPETLNAFRDRGKVPGATVKQGLEEAENALAKLATLGIDLDAI
                     TEKLQKDGVAAFKASFDQLMSGLEKKRHAMIGAELDRLELHLGTYQKRIDRRIAQWQK
                     AGIVSRIWKKDYTVWSKSPEPELTDRLGWLELPETMGEQCDKLSAFADQATADNIKDV
                     VLLGMGGSSLAPEVFQQTFGNAEGYPRLHVLDSTHPAAVKALESHIDLAHTLFIVSSK
                     SGTTTETNSFFYYFWDKLKQIEGEPGSHFVAITDPGTPLQHLATERRFRGIFNAPEEV
                     GGRYSALTVFGLLPAALIGVDVKEILARARRMSGACSATTAENLHPAVLLGAALGELT
                     LAKRDKVTFLCSPSLTGLPSWTEQLIAESTGKDRKGIVPVVSESLAAPETYGGDRFFV
                     YLRLDGDENHSLDRQTAALEANGHPLARIELKDKYDLGQEFFRWEFAVAAAGAVLGIH
                     PFNQPDVQLAKDLAKQAMSEADGKKTHKEKDNIAVGDNDR"
     gene            complement(2508..2966)
                     /gene="rpiB"
                     /locus_tag="VFY96_06100"
     CDS             complement(2508..2966)
                     /gene="rpiB"
                     /locus_tag="VFY96_06100"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX5606067.1"
                     /translation="MRIVVGADHAGFEMKQIIANYLRHIGHEVVDIGTNSDSPVDYPD
                     FAEALAKAISEGEAERGVLICGSGVGASVAANKIPGIRAGLCHDTYSARQGVEHDDMN
                     VLVLGARVIGVELARELVNNFLAAKYSDEERHHRRVAKIKALEQRYRKNT"
BASE COUNT          617 a          919 c          828 g          716 t
ORIGIN      
        1 agcgatcatt gtcacccact gcaatattgt ccttctcctt atgggttttt ttgccgtctg
       61 cctcactcat cgcttgctta gcgagatctt tcgccagttg cacatcaggc tggttgaagg
      121 ggtgaatgcc gagtaccgcg ccggctgcgg cgacagcaaa ttcccagcgg aaaaattcct
      181 ggccgagatc gtatttgtct ttaagctcga tgcgcgccag cggatgacca ttcgcttcga
      241 gggctgccgt ttgccgatcg agggaatggt tttcatcacc gtccaaacgc agatagacaa
      301 aaaatcgatc gccgccataa gtttccggcg ctgccaaact ttcgctgacc accggcacaa
      361 tgcctttacg gtccttgccg gtgctttcgg cgatcaattg ctcggtccag gagggtaggc
      421 cagtgagcga aggagaacag aggaacgtca ctttgtcccg ctttgcgagc gtcagctctc
      481 cgagggcagc gccaagaagc accgcgggat gcaggttctc ggccgtggtc gccgagcaag
      541 caccactcat ccgtcgcgcg cgcgccaaaa tttctttgac atcgacaccg attaaggcgg
      601 ccggtagcaa gccaaatacc gtcagcgccg agtagcgccc gccgacttcc tcgggcgcgt
      661 tgaaaatccc tcggaagcgc ctctcggtgg cgagatgctg caacggagtt ccggggtcgg
      721 tgatggcgac gaaatgcgag cccggctctc cctcgatctg cttaagcttg tcccagaagt
      781 aatagaagaa tgaattcgtt tctgtggtcg tgcccgactt gctcgagacg ataaacaagg
      841 tgtgcgccaa atcgatgtgt gactcgagag ctttaacggc agcgggatgg gtgctgtcga
      901 gaacatgaag ccggggatac ccctcggcgt tgccgaaggt ctgctgaaag acttccggcg
      961 ccagactcga accgcccatg ccgaggagca cgacatcctt tatattgtcg gctgtggctt
     1021 gatcagcgaa cgctgaaagt ttgtcgcact gttcacccat tgtttcaggt aactcgagcc
     1081 agcctaaacg gtcggttaat tcgggctcag gactcttgga ccaaacggtg taatcttttt
     1141 tccagattcg gcttacgatc cctgcctttt gccactgggc gatgcgccga tcgatccgtt
     1201 tctgatatgt gccgaggtga agctcgagcc ggtccaattc cgcgccgatc atcgcgtggc
     1261 gctttttttc cagacccgac atgagttggt cgaaagatgc tttaaacgcg gcgacgccat
     1321 ctttttgaag cttctccgta atggcatcca aatcgatgcc caaggttgcc agtttcgcca
     1381 atgcgttttc ggcttcctcc aagccttgct tcactgtggc gccgggaact ttgccacgat
     1441 ctcggaacgc attcagggtt tccggcggca tggtattcac ggtctccggt ccgattagat
     1501 tctcgacgta gagcacatct gaataggcgg gatttttcgt ccccgtgctt gcccataaag
     1561 gtcgctggac ccgcgcgccg cgctgccgca gcgcggcgaa accctcgcca tgaaaaatct
     1621 ctagaaaccg acggtagact accttggaat tcgcgatggc gatcttgccg gcgagcgcct
     1681 tggcttcggg gttgctgctc tcctcaaggg ctttatcaac ggccgtgtcg acgcggctga
     1741 cgaaaaagga ggccaccgag gccacgccgt cagggttgga acaccgctca agcccctgga
     1801 tgtaggcgcg cgcgacggct tcgtagtggt gcatggaaaa catcaaggtg acattgacgt
     1861 tcacgccgtt cgcgatcaat tcctcgatgg ccggcaggcc cgccggggtc gccggcactt
     1921 taatcatgac gttcggccgg tcgacggcgg ttctcagccg ctgcgcttcg ctaatcgttc
     1981 tctccgtgtc atcggcgaga tgcggcgaga cctcgaggct cacatagcca tcatctccgt
     2041 gcgtttcatc ataaaccgcg cgcaaaacgt cggctgccat ctgaatgtct tcgatcgcca
     2101 atctctcgta aaggtctccc gctggcgcgt gcagattatc cgcgagcaac gcccgcaacg
     2161 acatatcata gtccgtactg cccgaaatcg ctttgtcaaa aatcgtcggg ttgctggtga
     2221 ctccccgtaa cccgtcttct tccactaatc gtttcagctc gccgctccgg atgagatcgc
     2281 gcctgatgta gtccaaccaa atcgactgac cttgttccgc caattgtttt aaagaattca
     2341 tcttatctct ccctcaagag gtcgcgtcgc cccgtcatcc cgaggtcttc ggcttggctc
     2401 agaataaact cgccgaagga tgtcgctttg ttgtagcgaa acccttgggc gaaatccttc
     2461 actccagaat gacatcacag cgcaatgttg tggcgactgc cctcgaatta ggtatttttc
     2521 ctgtagcgct gctccaaagc cttgattttt gccaccctgc gatgatggcg ctcctcgtcg
     2581 ctgtattttg ccgctaaaaa attatttacc aattcgcgcg ccagctcgac tccgattaca
     2641 cgcgcgccga gcacgagaac gttcatatcg tcatgctcga cgccctggcg tgcggaataa
     2701 gtgtcatggc aaagcccggc gcggattccg gggattttat tcgccgcgac cgacgcgcca
     2761 accccgctgc cgcagatcaa tacgccgcgc tcggcctcgc cctcgctgat cgccttggcc
     2821 agcgcctcgg caaaatccgg ataatccact gggctatcgc tgttggttcc gatatcgacg
     2881 acctcatgtc caatatggcg caaatagttg gcgattatct gtttcatttc gaagccggca
     2941 tggtccgcac cgacgacaat gcgcatgact actttcccag cagtgctgtc gcgcgctcga
     3001 ccacgtgatc gacggtgaag ccgagctttt ccatgaccac gtttcctggc gccgaagcgc
     3061 caaatctttc cacgccgatg
//
LOCUS       DASULZ010000292         2589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_848755, whole genome shotgun sequence.
ACCESSION   DASULZ010000292 DASULZ010000000
VERSION     DASULZ010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848755"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..573)
                     /gene="rplJ"
                     /locus_tag="VFY96_06105"
     CDS             complement(31..573)
                     /gene="rplJ"
                     /locus_tag="VFY96_06105"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX5606068.1"
                     /translation="MSEAPLVRATHGEKAETIGAVHERFVSAKMAVVTEYRGLTVAQM
                     TRLRREIRDASGEYQVIKNTLVRRALDGTVFRDLEKLLEGPNGWVFAYEDPVVLSKAL
                     VKFADDHDKLTIKGGVFEGQFMDTAAVKALSQMPSKPELQAKLLAMINAPATHLVRLM
                     QEPGARVVRLLESVRKSKSE"
     gene            complement(624..1328)
                     /gene="rplA"
                     /locus_tag="VFY96_06110"
     CDS             complement(624..1328)
                     /gene="rplA"
                     /locus_tag="VFY96_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX5606069.1"
                     /translation="MSEKGKNYRAALNKVDRNQRYLLEDSLRLAKETARAKFDETVEM
                     AIRLGVDPRQADQNIRGTVTLPHGMGKSVRVLAFAKGEKEKEAQEAGADFVGSDELIK
                     KISEGWLDFDKAVATPDMMGAVGRIGKILGPRGLMPNPKTGTVAMDIGKAVKEIKAGK
                     LEFRVDKAGIVHVPVGKASFGPEQLIDNAKAVLGSIIRAKPASAKGNYIKGVTVATTM
                     GPGIKLDLAQIRAMAA"
     gene            complement(1339..1764)
                     /gene="rplK"
                     /locus_tag="VFY96_06115"
     CDS             complement(1339..1764)
                     /gene="rplK"
                     /locus_tag="VFY96_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX5606070.1"
                     /translation="MAKKVIGEIKLQIPAGQANPSPPVGPALGQRGVNIMEFCKAFNA
                     ATQAQQGMITPVIITVYADRSFTFVTKTPPASVLLKKAAGVERGSSETGKTTVGKVTR
                     AQVRQIAELKLKDLTAKNLAAAEKSIEGTARSLGLEVEG"
     gene            complement(1782..2312)
                     /gene="nusG"
                     /locus_tag="VFY96_06120"
     CDS             complement(1782..2312)
                     /gene="nusG"
                     /locus_tag="VFY96_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX5606071.1"
                     /translation="MAKQWYIVHTFSGYEQKAKAALEERIRALGKHDFFGEILVPVEK
                     VVELVKGKKKTSSRKFFPGYILVQMELNDDTWHVVKETPKVTGFVGGSTNPPSVTDEE
                     VRAITQQMEEGAIKPKPRVLFSVGENVKVIDGPFADFNGVVEEVKPDKGKLRVLISIF
                     GRATPVELEFVQVERN"
     gene            complement(2332..>2589)
                     /gene="secE"
                     /locus_tag="VFY96_06125"
     CDS             complement(2332..>2589)
                     /gene="secE"
                     /locus_tag="VFY96_06125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX5606072.1"
                     /translation="RFESCLTRHLMDFSMGDWIQTIQESARHAAEFFKEAWQELKKVY
                     WPSRKETYAATLVVIIVVVIISVYLAAVDLVLTKAIQAIIG"
BASE COUNT          519 a          780 c          631 g          659 t
ORIGIN      
        1 gcttccccct ttgttagtga tcaaaagatg ctattcagat ttgctttttc gaaccgattc
       61 aaggagccgc accaccctcg cgccgggctc ttgcatgagt cgcacaagat gtgtcgccgg
      121 agcgttgatc atcgcaagta atttggcctg cagttccggc ttgctcggca tctgggagag
      181 ggccttgacg gcggccgtgt ccatgaattg accttcaaag acaccgccct tgatcgtgag
      241 tttgtcatgg tcatcggcga attttacgag agccttcgac aaaaccaccg gatcttcgta
      301 ggcaaaaacc caaccgttgg ggccttccag cagcttttcc agatcgcgaa agactgtacc
      361 gtccagcgct cggcgcacca aagtattctt gatgacctgg tattcacccg aggcatcgcg
      421 aatttcacgc cgcagccgcg tcatctgagc gaccgtcagc ccgcgatact ctgtcaccac
      481 tgccattttg gcggatacga accgttcatg gaccgcgcca atggtttccg ccttttcacc
      541 atgagtggca cggacgagtg gcgcttcaga cattctgttc tcccaaactc atgcgagcga
      601 ttcagagact ggcaacagtt acctcaagcc gccatggcgc gaatttgcgc gaggtcgagc
      661 ttgatcccgg gacccatcgt ggtcgcaacc gttacacctt tgatatagtt acccttggcg
      721 gacgcgggtt tcgcccgtat aatcgatccg agcaccgctt tggcattatc gatcaactgc
      781 tcgggcccga atgaagcctt gccgacgggc acgtggacga taccggcttt gtcgacgcgg
      841 aactccagct tccccgcttt gatttcttta acagctttgc cgatgtccat cgctacggtc
      901 ccggttttcg ggttcggcat gaggccgcgc gggccgagta ttttaccgat ccgtccgact
      961 gctcccatca tgtccggcgt cgcgaccgct ttgtcgaagt cgagccagcc ttcgctgatc
     1021 tttttaatga gttcatcgct accgacgaaa tccgctccgg cctcctgcgc ttccttttct
     1081 ttctcgccct tggcaaaggc aaggacgcgc acactcttgc ccatcccatg cggcaacgtc
     1141 acggttccac gaatgttttg atcggcttgg cgcgggtcga ctccaagtcg gatggccatc
     1201 tccacggttt catcaaactt ggcgcgcgcc gtctcttttg ccaaccgaag actgtcttcc
     1261 agtagataac gttggttccg atctactttg ttcaatgcag cgcgatagtt cttgcctttt
     1321 tcgctcatca tttcctcatt aaccttcgac ttctaagcct aagctgcgcg ccgttccttc
     1381 gattgatttt tcggccgccg ccagattttt tgccgtgaga tccttgagct tcaattcggc
     1441 aatttggcgg acctgagctc gagtcacttt gccgaccgtt gtcttgccgg tttctgaaga
     1501 gcccctttcc accccggcgg ccttcttcag gagcacagag gcgggaggtg tttttgtcac
     1561 aaacgtaaaa gagcggtccg catacacggt aataatgacc ggcgtgatca ttccttgctg
     1621 cgcctgcgta gcggcgttga acgctttaca aaactccatg atattgacgc cgcgttgccc
     1681 cagagctgga cccacagggg gactcggatt ggcctgcccc gccgggatct gcaacttgat
     1741 ctcgccgatc actttttttg ccattggctc aatccttcca actaattgcg ttcgacttga
     1801 acgaactcaa gctccactgg agtcgctcgg ccaaatatgc tgatcaagac tcgcagcttc
     1861 cccttgtcgg gtttgacttc ttcgaccact ccgttgaaat cggcaaatgg accatcgatc
     1921 accttgacgt tttcacccac cgagaacaat acccgtggct tcggtttgat ggcgccctct
     1981 tccatctgtt gggtaatggc cctgacttcc tcatccgtca ccgaaggagg attcgtactg
     2041 ccgcccacaa accccgtcac cttcggtgtt tcttttacga cgtgccaggt gtcgtcattc
     2101 agttccatct gaaccagaat gtatccggga aaaaacttgc gcgaggacgt cttcttcttg
     2161 ccttttacca actcgacgac cttctcgact ggcaccagta tctcgccgaa aaaatcgtgc
     2221 ttcccaaggg cccttatgcg ctcctctagc gcggccttcg ccttctgttc atatcccgaa
     2281 aacgtgtgaa cgatgtacca ctgcttggcc atagcgggcg cccgaaatgt atcagccgat
     2341 gatcgcctga atggctttgg tcaagaccag atcgaccgcc gccaaataga ccgaaataat
     2401 gacgacaaca ataattacca cgagagtcgc tgcgtaagtc tccttacggg aaggccagta
     2461 gacttttttc aattcctgcc atgcttcctt aaagaactcg gcagcgtgcc gggcactctc
     2521 ttggattgtt tggatccaat cacccatgct gaaatccatg agatggcggg tcaggcagga
     2581 ctcgaacct
//
LOCUS       DASULZ010000293         5586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1456697, whole genome shotgun sequence.
ACCESSION   DASULZ010000293 DASULZ010000000
VERSION     DASULZ010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1456697"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VFY96_06130"
     CDS             complement(<1..742)
                     /locus_tag="VFY96_06130"
                     /inference="COORDINATES: protein motif:HMM:NF015089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX5606073.1"
                     /translation="MNAASTLLPIFQKNEVLFGHDGTAEIVAVEITAQGAVQISAHDG
                     ERIFSEPMPFHPFMLLAGDDALAGWPGEAQIEILAGESQFNRLAQFRNLRELDEARWF
                     LQKKTGKTPSSSDAPYWYFNDPLHQYLLRSGKTHFLEMTFGDLRRLQLTIQTYCAEGF
                     SFSNSARPSDRIVTIGLSDSTGWERIISANDYDEAEMLRQFVDEIRLRDPDVIEGHNL
                     LRFDLEYLEARARRHKVALNLGRDGSRLS"
     gene            916..1650
                     /locus_tag="VFY96_06135"
     CDS             916..1650
                     /locus_tag="VFY96_06135"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606074.1"
                     /translation="MVNNFSQAALAPTGTLRVGINYGNPVLARRDLASGELSGVAVEL
                     ARELARHVEIPLSLVAFESAGKMFDAVKDDAWDVAFLAIDPARADQIDFTPPYLEIEG
                     TYLVPESSQIASIDEVDRSGVRIGVSANSAYDLYLRRAIAQAELIRASNPDAAFQLLI
                     DDKVDVIAGVRQHLVANSSKLPGSRVFHGRFMAIQQALGIRKGRQATEALSDFVEQAK
                     SSGLVARIIENAGVRGVSIPQPESIG"
     gene            1821..2801
                     /locus_tag="VFY96_06140"
     CDS             1821..2801
                     /locus_tag="VFY96_06140"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606075.1"
                     /translation="MRKVGWLRGLLFCSLILPVVAHAASDAKTVKMVIPRGSTFVLSY
                     FGARDAGIFRKHGIDIEVDARPFAGFMAGLPSRQVSVGTYAGLNAVEQMNQGKDLIVI
                     GGGLTSMQDVWVQKDSPLKKLSDLKGKKFGVWSRGAGATKALEALAIDGFKLDLNKDV
                     QTIEIAGPALMALLDKGEVDAMFNLSSLSIAAASQPEKYRLLFSPDDYWREKTGSPLV
                     WSAPTIAWKDWIDQDRERAKNIVEALHESWRWLRQNSDKAVEKYGKLAGVQNAAEAAT
                     LKKMLQAGGIFLDKWDPKIVDSQWKFLDLAKQVGIIKAVPAKDKYSLVLR"
     gene            2847..3578
                     /locus_tag="VFY96_06145"
     CDS             2847..3578
                     /locus_tag="VFY96_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5606076.1"
                     /translation="MLSVIGGLLLWQAVSQFFLPTFLPSPLTLLGIIVSIFSKPSAYL
                     IIGKTLQRVVAGVVFSMFIGTALGLVMGLRRSVANFFDSWVMVLLTFPAISWAFLSVL
                     WFGLSDIAPIFTVVLIVFPYVTMNVWEGTKALDKELTDMAAVFRANRSLLLRKVMVPQ
                     LMTYLFSSLRISFALSWKIALVSEAFGAGVGVGKELTYWFQASRADMMLAWGASFMAL
                     MVLIEMLVLRRWETQVFAWRQPAAT"
     gene            3583..4359
                     /locus_tag="VFY96_06150"
     CDS             3583..4359
                     /locus_tag="VFY96_06150"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5606077.1"
                     /translation="MASQAVEIQELSKKFPSPNGLGQFTVIESLSLQIARGSFVSIVG
                     PSGCGKSTLLNIIAGIEPRDGGEVRIAAQHGNTAQARIGYVFQSPRLLNWLTVEDNLK
                     FALEAQKIERAGWNERVAKYLKMVGLTGQERNFPLNLSGGMQQRVAIARALAIEPDIL
                     LMDEPFSHLDEITARGMRVELVRILEHARPTILFVTHSLREAVFLAEEVYMMSVKPAR
                     IFKHLHVDIPRPREPEDPRLFDLEKDLVKEFFAVLEQDQR"
     gene            4356..5126
                     /locus_tag="VFY96_06155"
     CDS             4356..5126
                     /locus_tag="VFY96_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5606078.1"
                     /translation="MNKQIALRFLSIVSFLAVWWIGSLFSAPEILPSPAAIGATIVDN
                     FTTPGPEDKTAEFHIGVTLARIFFTFTVAMALGIGLGLLMGLRPKIELAFGWLLPLTL
                     TIPTLLAVFLCVMWFGYSEAGGLFAVILTVTPFVVVNMLAGTKALDKDLIDMARAFKA
                     NRRLMIRKVLLWQLMPYIFAAFRYAFGMTWKIVALAETFGLKYGVGYMFTFWFEQFNM
                     TQVMAWIVLFVALMLILEHGIIARIEDKIFAWRPVHAF"
     gene            complement(5136..>5586)
                     /locus_tag="VFY96_06160"
     CDS             complement(5136..>5586)
                     /locus_tag="VFY96_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606079.1"
                     /translation="AAMVTIPTSYEAKEDGLNVLANISKYLPDYQFTVIAGNEKWMAS
                     HKDQTVRFLMAMTKAMRFLNDPKNKEASIQAMVKHFKVKRKYAELAYQEVMQQLRPID
                     NNAAPSIKGMETVINLEVENKGLDKSYPPQHFIDDSYRQETLKRLGK"
BASE COUNT         1177 a         1482 c         1585 g         1342 t
ORIGIN      
        1 cgctgagtcg cgagccgtcg cggccgagat tgagcgctac tttatgccgc cgggctcggg
       61 cttcgaggta ttcgagatca aagcgcaaca gattatgacc ctcgatgacg tcgggatcgc
      121 gcagccggat ttcatcgaca aactgacgca gcatttcggc ttcgtcatag tcgtttgcgg
      181 aaatgatgcg ctcccacccg gttgaatcgg acagtccaat cgtcacgatc cgatcgctcg
      241 ggcgcgccga attagaaaaa ctgaatcctt ctgcgcaata agtctggatc gtgagttgca
      301 ggcggcggag atcgccaaac gtcatttcga gaaaatgagt ttttcccgac cgtagcagat
      361 attgatgcaa tggatcgttg aagtaccagt agggcgcgtc ggatgaagag ggtgtcttgc
      421 cggttttttt ttgtaaaaac catcgcgctt cgtccagctc tcgaagattt cgaaactgtg
      481 ccagtcggtt gaactggctc tcgcccgcaa gaatctcgat ctgtgcttca cctggccatc
      541 ctgccaaagc atcgtctcca gccagcaaca taaacgggtg aaacggcatc ggctccgaaa
      601 aaatcctttc gccatcgtgg gcagagatct ggacagcgcc ttgtgcagtg atttccacgg
      661 caacaatttc ggcagttccg tcatgaccga aaagcacttc gtttttttga aagatgggca
      721 acagtgtcga tgcggcattc ataacgtgtt gccactatag caaaccggct tcagaactca
      781 tactctcgtt catcgctcac tgcactgttt tggctatctc cagattcggt tgacaccgat
      841 gcgggcggat gaataatctg aaaaatccgc tgaacggcgc gggcttgaaa ctcattagac
      901 tggatggatt gcgccatggt gaataacttt tcacaagcag ctctcgcgcc cacaggaacg
      961 ctgcgcgtcg ggatcaacta tggaaacccc gtgctggcga ggcgagactt ggcgagcggg
     1021 gaactcagtg gagtggcggt ggagctggcg cgcgagctgg ctcggcatgt tgagataccc
     1081 ctcagtctcg tcgccttcga atcggctgga aaaatgttcg atgcggtaaa agacgatgcc
     1141 tgggatgtcg catttcttgc gatcgatcca gctcgcgccg accaaattga tttcacgcct
     1201 ccttatcttg aaatcgaagg cacatacctg gtgccggaga gctctcaaat cgcatcgatc
     1261 gacgaggtag atcgttcagg agtacgcatc ggggtgtcgg ccaatagcgc atacgattta
     1321 tatttgcgcc gtgcgattgc tcaggccgaa ttgattcgtg cttcgaatcc cgatgcagct
     1381 tttcagttgt tgatcgacga caaagtcgat gtcattgcgg gcgtgcgaca gcatcttgtc
     1441 gcgaactcga gcaaactgcc cggatcgcgg gtatttcacg gcaggttcat ggcgatacaa
     1501 caagcgctgg ggattcgcaa aggccggcaa gcgactgaag ctctgagtga ttttgtcgag
     1561 caggcaaagt cttccggatt ggtggcgcga attatcgaaa acgccggtgt acgtggcgtt
     1621 tccattcccc aaccggaatc cattggctaa acgccgttct ctacgcactc gcctcaaaaa
     1681 gttttcattt gcaccttctt gaccgaagta cggcggattt ataggcttcg accaactcgc
     1741 cgattttcag gccgttgcct acgatggtgc tgaatagagc tgaatcaaat cggtgacagg
     1801 gcgaatgaaa ggagactgaa atgagaaagg tcggctggct cagaggcttg ttgttttgct
     1861 cattgatctt accggtggtc gcccacgccg cgtcggatgc gaagaccgtg aaaatggtga
     1921 ttccaagagg gagcacattt gtcttgagct acttcggcgc gcgcgatgcg ggcatattcc
     1981 gcaaacacgg cattgacatc gaggtggacg cccgtccgtt cgcggggttt atggcgggtc
     2041 ttcccagccg ccaagtatcg gtcggcacat acgccgggct caatgccgtc gagcagatga
     2101 accaaggtaa agacctgatc gtcatcggcg gcgggctaac atctatgcag gacgtctggg
     2161 tgcagaagga ctcgccgcta aagaaattat ccgatctcaa aggcaaaaaa ttcggcgtgt
     2221 ggtcgagagg cgccggcgcg accaaggcac tcgaagcgct ggcgatcgac ggcttcaagc
     2281 tcgaccttaa caaagacgtg cagacgatcg aaatcgcagg gcccgccttg atggctctgc
     2341 tggacaaagg cgaggtcgac gccatgttca atctgagctc tctctcgatc gccgcagcat
     2401 cacaaccgga gaaataccga ttgctcttca gccccgacga ttattggcgg gaaaagacag
     2461 gctcgccctt ggtctggtcc gcgcccacga tcgcctggaa ggattggatc gaccaagacc
     2521 gcgagcgcgc gaaaaatatc gtcgaggcgc tgcatgagtc ctggcgttgg ctgcgccaga
     2581 attcggacaa ggcggtggaa aagtacggca agctcgccgg cgtgcagaat gccgccgaag
     2641 cggcgaccct caagaaaatg ctccaggcag gcggcatctt tctcgacaaa tgggatccga
     2701 agatcgtcga ttcacagtgg aagttcctcg atctggccaa acaggtcggc atcatcaaag
     2761 ccgtgccagc gaaggacaag tactctctgg tcttgcgcta gtcaaaatgt cacgtgaagt
     2821 ttattctccg cagtaaatta cctgttgtgc tttcggttat tggcgggctc ttgctatggc
     2881 aggcggtctc acaattcttt ttgccgacat tcttgccctc gccgctgaca ctgctcggca
     2941 tcatcgtgtc gatcttcagc aagccttcgg catacctgat tatcggtaag acgctgcagc
     3001 gagtggttgc aggcgttgtc ttttccatgt tcatcggcac agcgcttggt ctcgttatgg
     3061 gattgcgccg ctcggtggca aatttctttg atagctgggt gatggtcctg ttgaccttcc
     3121 cggcaatttc ctgggccttt ctttccgtgc tctggttcgg gctgtccgac atcgcgccga
     3181 ttttcacggt cgtgctgatc gtctttcctt acgtcacgat gaacgtttgg gagggcacga
     3241 aagcgctgga taaagaactc acggatatgg cggcggtttt tcgggcaaac cgttccttgt
     3301 tgctgcgcaa ggtcatggtt ccacagctga tgacatatct tttttcgtca ctccggatca
     3361 gcttcgcgct atcgtggaaa atcgccctgg tgagcgaagc attcggcgcg ggcgtcggtg
     3421 tcggtaaaga gctgacgtat tggtttcaag cgagccgcgc cgacatgatg ctcgcctggg
     3481 gcgcgagctt catggccttg atggtcttga ttgagatgct ggttttgcgg cgttgggaaa
     3541 cccaggtgtt tgcttggcga caaccggcgg caacctgaaa acatggcgtc tcaagccgtc
     3601 gagatccaag aactgagcaa gaaatttcca agccccaacg ggctcggtca gttcaccgtc
     3661 atcgaatcgt tgagtctgca aatcgcgcgt ggcagctttg tgagcatcgt cggtccctcg
     3721 ggctgcggta agagcacgct actcaatatc atcgccggta tcgagccgcg tgatggcggc
     3781 gaggtgcgca tcgccgcgca gcacggcaat acggcgcagg cacgcatcgg ctacgtattt
     3841 cagagcccgc gcttgctcaa ctggcttacc gtggaagaca atctcaaatt cgccctggaa
     3901 gcgcagaaga tcgagcgcgc tggctggaac gagcgagtgg caaaatatct caagatggtc
     3961 gggctaaccg gtcaggagcg caactttccg ctcaaccttt ccggcggtat gcagcaaaga
     4021 gtcgcgatcg cgcgtgcgct tgcgatcgag ccggatattt tgctgatgga cgagccgttc
     4081 agccatctcg acgagatcac cgcgcgcggc atgcgcgtcg agctggtgcg gattctagag
     4141 cacgcccgcc cgacgattct gttcgtcacg cattcgctgc gcgaggcggt gtttcttgcg
     4201 gaagaagttt acatgatgag cgtcaagccg gcgcggatct ttaagcactt gcacgtcgat
     4261 atcccgcggc cgcgcgagcc cgaggacccg cgcttgttcg atctggaaaa ggatctggtc
     4321 aaagaattct tcgcggtgct ggaacaggat cagcgatgaa taagcagatc gcgctgcggt
     4381 ttctatcgat cgtttccttt cttgctgtct ggtggatcgg atcattgttc agcgcgccgg
     4441 agatcttgcc cagcccggcg gccatcggcg caacgatcgt ggataacttc accacgccgg
     4501 ggccggaaga caagaccgca gaattccaca ttggagtaac actggcgcgc atctttttca
     4561 catttaccgt cgccatggcc ctaggcattg gtctcggctt gctcatgggt ctgcgcccca
     4621 aaattgaact ggccttcggt tggttgttgc cgctgacctt gacgatccca acgctcttgg
     4681 cggtgtttct ctgcgtcatg tggttcggct acagcgaggc agggggtctg ttcgcggtca
     4741 ttctgaccgt aacccctttc gtcgttgtca acatgctcgc cggaaccaag gcattggaca
     4801 aagatctcat cgatatggca cgcgctttca aagccaatcg ccgcctgatg atccgcaagg
     4861 ttcttctctg gcagctcatg ccttacattt tcgccgcgtt ccgctatgcc ttcggcatga
     4921 cctggaaaat cgtcgctttg gccgagactt tcgggctcaa atacggcgtc ggctacatgt
     4981 tcacgttctg gttcgagcag ttcaacatga cccaggtgat ggcctggatt gttctcttcg
     5041 tcgcattgat gctgattctt gagcacggca ttatcgcgcg aatcgaagat aaaatctttg
     5101 cctggcggcc tgttcatgcc ttctgaggcg aagtgttatt ttccgagccg cttgagcgtc
     5161 tcctggcgat agctgtcgtc gataaagtgc tgcggcggat aagatttatc cagccctttg
     5221 ttttcgacct cgagattgat gacggtttcc atgcctttga tgcttggcgc tgcattattg
     5281 tcgatggggc gcagctgctg catgacttct tgataggcga gctcagcgta ttttctcttg
     5341 accttaaaat gcttaaccat tgcttgaatc gaggcttcct tattcttcgg atcgttgaga
     5401 aatcgcattg ctttggtcat cgccatgaga aagcgcaccg tctgatcttt gtggctcgcc
     5461 atccactttt cgtttccagc gatgaccgtg aactgataat cgggaaggta tttcgaaatg
     5521 ttggcaagca cattcaagcc gtcttctttg gcttcgtaag aggtcggaat cgtaaccata
     5581 gcggcg
//
LOCUS       DASULZ010000294         2953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_849141, whole genome shotgun sequence.
ACCESSION   DASULZ010000294 DASULZ010000000
VERSION     DASULZ010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849141"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            366..1124
                     /locus_tag="VFY96_06165"
     CDS             366..1124
                     /locus_tag="VFY96_06165"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012306307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX5606080.1"
                     /translation="MRPHAQRYRNPIRTAGLPLAGIILFVSVACGFSEPERDTEAWDE
                     ARKRMVDTQLISRDIRDTRVIDAMHKVPRHWFVPTAERLHAYIDSPLPIGYSQTISQP
                     YIVAYMTQALEVRPTDHVLEIGTGSGYQAAVLAELAHQVYTIEIVAPLAQQARETLAS
                     LGYRNIHVRTGNGYLGWEEHAPYDRIVVTAAPEEVPPALVQQLKIGGLMAIPVGRFFQ
                     ELKILRRTPGGMETLRTLPVRFVPMTGNSSQDAK"
     gene            1496..2032
                     /locus_tag="VFY96_06170"
     CDS             1496..2032
                     /locus_tag="VFY96_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606081.1"
                     /translation="MSHAKSLRVIGQFLDIASVPSFELEKERHAQDYLLRSDSLTKTR
                     EWILRHARDENDSIEESRRPVADDGLLRFTPIDISRLESQGQRKRRAHSSAQTQGSKN
                     LSQLLRSLGDHLDRIKASAFHVTWTPDSVSVDYQKADGLSDSRQFSPERLRQLDLHTR
                     FRRSSGTGQTASGRQFIR"
     gene            complement(2459..>2953)
                     /locus_tag="VFY96_06175"
     CDS             complement(2459..>2953)
                     /locus_tag="VFY96_06175"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5606082.1"
                     /translation="DAQASFFLIGDRALRHSEVVSRIKAGGHEVGNHYFMDTSVLGHS
                     DAAFLDYLKQTEKAVGIETRPKLFRPPGGVAWPRQLRVARKEGYVCVLGSAYANDPIH
                     PPVWYIKWWIEKNLVPGAIIILHDGILDPRRGIMALPHILAAGCQKGLRFVAVGTLMK
                     SAVE"
BASE COUNT          694 a          816 c          797 g          646 t
ORIGIN      
        1 caccgctcaa gaacttggtc ttcaaacgac acgataacca gcagaccaaa aatccattgt
       61 ttaaagccag caaccgatta acgtaatccg aaggcaaaat ccctttgatg gcaggtatat
      121 ttggcatctg gcctacctgg caaccagaac acgttcagac acggcttaac gggcatccgc
      181 agcgccgcgc tgagggcgtt gtcacgaacg gccctccgct tgccggcggc atttgccgat
      241 attaatatcc gcaaaagctg gtcagtatat tacggttgga tgcggtaagt agcctgttga
      301 ccccagtctg atggtttatc gtatcgatgt cgttgccaat ctgtctgcta gtcggcgcgg
      361 ctaacatgcg accccacgcc caaagatatc gaaatccgat ccgtacagct ggtttaccac
      421 tagctgggat tattttgttc gtctcggttg cgtgtggctt cagcgaaccg gagcgcgata
      481 cagaggcatg ggacgaggcg cgcaaacgga tggtcgatac gcagctgatc tcacgcgaca
      541 ttcgcgatac gcgggtgatc gacgcgatgc acaaggtacc gcgccattgg ttcgttccga
      601 cagcggagcg tttgcacgct tacatcgact ctcccctgcc gatcggctac agccagacga
      661 tctcacaacc gtacatcgtc gcttacatga cccaagccct tgaggtgcgc ccgaccgatc
      721 atgtgctcga gatcggcacg ggctcgggct atcaggctgc ggtgctcgct gagctggctc
      781 atcaggttta caccatcgaa atcgtcgcgc cgctcgcgca acaagcccgc gaaacgcttg
      841 ctagcttggg gtaccgcaac attcacgtgc gcaccggcaa cggctacctc ggttgggagg
      901 aacacgcacc ctatgaccgg atcgtggtga cagcggcacc cgaagaagtg ccgcccgcgc
      961 tcgtgcagca gctcaagatc ggcggcctga tggcaatccc cgtaggccgg ttttttcagg
     1021 agttaaaaat cctgcgccgg acgccgggcg gaatggaaac gctgcgcacg ctgccggttc
     1081 gctttgtgcc aatgaccgga aattcgagcc aggatgcgaa atagcgcaaa cgttgttagc
     1141 gccctggtag cgtctatgta cattagaaca cttgtgcatt gagctcagaa caaatcagcc
     1201 gccacgagga aacgcacatg tgccttgctc gtgtgcatta ttgcagtttt gacttttgtt
     1261 ccgggtcgtt tatcttagct ggctcgatgg aaatgccgct tggcttggac cgttggatag
     1321 ccttccgtgc aaagtgttgc actctcgcgt caatgacggc cccctacgaa tttaaacgtt
     1381 ggcgatttct gcacccccat tgaaaactcc ttgaaatcgg caaaactgag cattgtaggt
     1441 cttcacatct ccgtacgacc tcatcgcggt ctacaattcg ttggatgtga caacgatgag
     1501 tcatgctaaa tcattgcgag ttattgggca gtttctagac atcgccagcg tgccaagctt
     1561 cgaattggag aaagaacgcc atgcgcaaga ttatctactc cgaagcgatt ccctgactaa
     1621 aacacgcgag tggatattgc gacacgcccg tgatgaaaac gattctatcg aggaaagccg
     1681 tcgcccagtg gcagatgatg gcctgttgcg cttcaccccg attgatattt cccggctcga
     1741 gtctcaaggg caacggaagc gccgcgccca ttcctcggca caaacgcaag ggtcaaaaaa
     1801 tttatctcag ctgttgcgga gcttgggcga tcaccttgac agaattaagg ccagtgcttt
     1861 tcacgtgaca tggacaccgg attctgtttc tgtagattat cagaaagccg atggcttgag
     1921 cgattctcga cagtttagtc ccgagcgact gcggcagttg gatttgcata ccaggttccg
     1981 gcggtccagc ggaaccggcc aaacggcatc tggccgccaa ttcattcgat gaagttagtt
     2041 cccgcaacgg gagtcgccaa gatcaatcca accccctcga gaagcattca cgtttattat
     2101 cgtcctcggg tatttgagca tccagcgggg aattgatcga tgtaacgatc aactcaagta
     2161 ccgcagtgcg atgatccagt tggctgcgga agcgaatgga tcaaagcgcg actcgatatg
     2221 acggcaaacc gaaacaggag tatttgtttc cgctggatga ggaaatcgct gagcggagca
     2281 aacctctatc gcggctgcgc cacaagcaaa gaggatgttg cgcctggagt ccatccagga
     2341 ggggcggtgc gaatccgacc gtggcgctcc aacccgcgtt cacgtcgcat tgaatgaaac
     2401 gatagcaggt gattaactga cagactcaca aagcatcgct tattgtggcc ctatccactt
     2461 attcgacggc acttttcatt agagtgccga ctgcgacgaa gcgcagacct ttttggcacc
     2521 ccgcagcaag gatgtgcggc aaagccataa tcccccgcct tgggtccaga atgccgtcgt
     2581 gaaggatgat gatagctcca ggaacgagat tcttttcaat ccaccatttg atgtaccaaa
     2641 ccgggggatg gatcgggtca ttagcgtacg ccgagccgag gacacacacg tagccctctt
     2701 ttcgggcaac tcgaagctgc ctgggccatg ccacgccgcc cggtgggcgg aagagcttcg
     2761 gcctcgtttc aataccgact gctttctcgg tctgcttcag ataatccaaa aaagcggcat
     2821 cagagtggcc cagaacggag gtatccatga aataatggtt gccgacttca tgtccgcccg
     2881 ccttaatacg cgaaaccacc tctgagtggc ggagagctcg gtcgccgata aggaagaagc
     2941 tggcctgggc atc
//
LOCUS       DASULZ010000295         4714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_646138, whole genome shotgun sequence.
ACCESSION   DASULZ010000295 DASULZ010000000
VERSION     DASULZ010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646138"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VFY96_06180"
     CDS             complement(<1..749)
                     /locus_tag="VFY96_06180"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX5606083.1"
                     /translation="MAYGDLREYVSTLEKHGKLKRVSKEVDKDWEIAAVCRQLFKKIP
                     PQKRPALMFENVKGFNIPVVAGVLGASREIYALGLQTETVEGINRKWDQALENPVPPR
                     MVKTAPCKENILHGKDVDITKLPVPVWTVGEDPAPFFTSPYLITKDPETGVRNVGTYR
                     MQVKGPNKTGLLIGKRQDMAWHVYKNNAQNKPTPLAVVIGADPSIGYVSVSKMSEALD
                     EFAVAGALRGQPVDLVPCETVPLEVPASAEIV"
     gene            complement(879..1805)
                     /locus_tag="VFY96_06185"
     CDS             complement(879..1805)
                     /locus_tag="VFY96_06185"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5606084.1"
                     /translation="MTVVFWKELADHFSSRRFMILLIIIILSGLWATYATGQAIRQNT
                     DTIPPQYAFLLLLTSSGETLFSLATFLGFFGPLVGITLGFDSISGEYARGTLSRVLSQ
                     PIYRDSLINGKFLAGLTTVAVLWGSILLLVIGLGITLLGFAPNAEEIWRMLIFTTVGI
                     FYVGFWLALAMLFSLVFQKTVTAALASIAVWLFLTLFVSVFSSAISGMIVPDATTPEA
                     LARRSDVENVIDRISPSTLFSESVGILLNPAARVFGMALQSQAEGILPTPLALSQSLN
                     LIWPHLTTMFALVAVCFGISYIIFMRAEIRAS"
     gene            complement(1807..2739)
                     /locus_tag="VFY96_06190"
     CDS             complement(1807..2739)
                     /locus_tag="VFY96_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5606085.1"
                     /translation="MSAVVETKNLTKRYREKLAVNSLNLTVEEGEVFGFLGPNGAGKT
                     TTILMLLGLTEPTSGLVSVCGFNPTRESLEVKRRVGYLPESPGFYDDISAKENLLYMA
                     RLNRIPDDEARRRTAQVLDQVGLPDDARRLVREFSRGMKQRLGIAEVLVKNPKAIILD
                     EPTLGIDPDGAIRILELIKSLNREHGLTVLLSSHQLQQVQEICNRVGIIVKGKLIVQG
                     EMDELGKSILKGRQWNFLLEVSGNTDGLERDLQSIPGVDEIEKRSHGWFLRCTQDVRG
                     EVVSLVTRKGVRLLQLRSEDPTLEEIYLKYFREA"
     gene            complement(2736..3953)
                     /locus_tag="VFY96_06195"
     CDS             complement(2736..3953)
                     /locus_tag="VFY96_06195"
                     /inference="COORDINATES: protein motif:HMM:NF022100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HEX5606086.1"
                     /translation="MLFLLLLVSGGFSARGNAAETGTPAPSKDAPASKESFNLILPFK
                     DLTVGQGQEATMDAEVVNRTKEPVEVSLTIEGVPSGWDVNFNSRYPSFPVRSVMVQGG
                     DQTANKSTTLEFKAKIPDSAKPGTYPVKVTAKDSKGSTQYTETINYRVTSKKVDTGGI
                     KISSQYPVLTSASGQTLKFTIDLKNETNKPLPTNIVAQAPQGWTVRFKPQFGDQQISS
                     IQLKENGSETLSVEIDTPASAEAKEYPITIQARAGAFEAAANVKVSLKGTQDLKMGSL
                     AGTLNTSVIAGTKTPIDFVIGNAGTAPIRNLNFVTKKPSDKWTVEFRPDKIDALGPGE
                     VRQIKMEILAPERTIAGDYMLTLTANSPETNKSADFRVTVSTPTIWGWIGFAIVGLVV
                     LGLAAVFFRLGRR"
     gene            complement(4076..4570)
                     /gene="trmL"
                     /locus_tag="VFY96_06200"
     CDS             complement(4076..4570)
                     /gene="trmL"
                     /locus_tag="VFY96_06200"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEX5606087.1"
                     /translation="METKADLLELHQLAVHIVLFEPEIPPNTGSVARLCAATLTPLHL
                     IEPLGFKIDDKHLKRAGLDYWEFVDLRVHSCWDAFLSNARPERMLFFSKRAVKSYTTV
                     RYGENDFLVFGPETRGLPQALLDANPDAALRIPMMGSGVRSLNLSNAVSIVLYEGLRQ
                     LGKT"
BASE COUNT         1100 a         1285 c         1256 g         1073 t
ORIGIN      
        1 acaatttcgg cgctcgccgg cacttccaac ggcaccgttt cgcacggcac cagatccacg
       61 ggttggccgc gcaacgcgcc cgccacggca aactcatcca aagcctcgga cattttggat
      121 actgatacgt agccgatgga cggatcggcg ccgatcacga cagccagcgg tgtcggtttg
      181 ttctgcgcat tatttttgta aacatgccaa gccatgtcct ggcgtttgcc aatcaataga
      241 cctgttttgt tcggtccctt gacttgcatc cggtaggtac ccacgttgcg gacgccggtc
      301 tccgggtctt tagtgatgag gtagggcgaa gtgaaaaagg gcgcgggatc ttctccaacc
      361 gtccacaccg gaaccggtaa cttggtaatg tcgacatctt tgccgtgcag aatgttttct
      421 ttgcacggag ccgttttaac cattcgcggt ggaactggat tctccaacgc ctgatcccat
      481 ttccggttga taccttcgac ggtttcggtc tggagcccaa gggcgtagat ttctctcgat
      541 gcgccgagca cgccggcgac aaccggaatg ttgaagccct tgacgttctc gaacatcagc
      601 gcaggacgct tctgcggggg aatttttttg aagagctgac ggcacactgc ggcaatttcc
      661 caatctttgt cgacctcctt gctgacccgt tttagctttc catgcttctc caatgtcgag
      721 acatattctc tcagatctcc gtaggccatt agttccccca attctaagag cagaaaattt
      781 cctgtatcct aaagggaaat tgtttatgta tcaacaattc caccaaaaac acggcgaccg
      841 tgaaaaaaga agaaccgatc gtgcttagat cgaggaaatt aggatgcgcg aatttcggca
      901 cgcataaaaa tgatgtacga gatgccgaaa cagactgcca cgagcgcaaa catggtcgtc
      961 aaatggggcc agatcaaatt caggctctga ctgagagcca gtggtgttgg caatatcccc
     1021 tcagcctgac tctgcagtgc catgccgaaa acccgagcag caggattgag taggatgccg
     1081 acactttcgg agaagagcgt gctcggggaa attcgatcga tgacattttc aacgtcggat
     1141 cgccgcgcga gcgcttcggg ggttgtcgca tccgggacga tcatgccgga aattgccgag
     1201 ctgaacaccg aaacaaagag ggtcaaaaac agccataccg cgatggatgc caaggccgca
     1261 gtcacggtct tctggaacac cagtgaaaac agcatcgcca gagcgagcca gaacccgacg
     1321 taaaaaattc ccaccgtggt aaagatcagc atgcgccaga tctcttcagc gttcggtgca
     1381 aagcccaata gcgttatccc caggccgatc acgagcagca aaatggagcc ccataacacc
     1441 gcaaccgtcg tcaagccggc caggaattta ccgttgatca agctatcgcg atagatcggc
     1501 tgagagagca cccggctcaa tgtgccgcgg gcgtactcgc cgctgatcga gtcgaatccc
     1561 aaggtgatgc cgaccaacgg gccgaaaaag ccgagaaatg ttgccagaga gaaaagcgtc
     1621 tcgccgctcg aagttaaaag aagcagaaac gcatactgcg gcgggatcgt gtcggtattt
     1681 tgacgaatcg cttgcccggt cgcgtacgtc gcccataggc cggacaaaat aatgatgatc
     1741 aagagaatca tgaagcggcg gctgctgaaa tggtcggcga gctctttcca gaacaccacg
     1801 gtcattttat gcctcgcgaa agtatttgag atagatttct tccaaggtcg gatcttccga
     1861 gcgcagttgc agcaaacgaa ctcccttgcg ggtgaccagc gaaaccactt cgccgcgcac
     1921 atcttgcgta cagcggagga accagccgtg gctgcgtttc tcgatttcgt ccacgccagg
     1981 aatactctga agatctcgtt ccaatccgtc cgtgttgccg ctcacttcca gcagaaagtt
     2041 ccattgacgg cccttgagaa tggatttgcc gagctcatcc atttcgcctt ggacgatcag
     2101 tttgcctttg acgatgatgc cgacccgatt gcagatttcc tgaacctgtt ggagctgatg
     2161 cgatgacaac aggaccgtca ggccgtgttc ccgattgaga ctcttgatca gttctaaaat
     2221 gcgaatggcg ccgtccggat cgattccgag agtgggctcg tcgaggataa tcgctttagg
     2281 gtttttgacc aacacttccg cgatcccgag gcgttgcttc atgccgcggg aaaactctcg
     2341 caccagccgc cgtgcatcgt cgggcagccc cacctgatcc aacacctgtg ccgtgcgccg
     2401 gcgcgcctca tcatcgggga tacgattgag tcgcgccatg taaagaagat tttcttttgc
     2461 gctgatgtcg tcgtaaaacc cgggactctc cgggaggtaa ccgacgcggc gcttgacctc
     2521 gagggattca cgggttggat tgaagccgca cactgaaacc aggccagagg tcggttcagt
     2581 cagtccgagc agcatcagaa tcgtggttgt cttgccagcg ccgttgggac cgagaaaccc
     2641 aaatacctcg ccttcctcaa cggtcaggtt gagcgaattg accgcgagct tttcgcggta
     2701 ccgcttagtt aaattttttg tttcaacaac cgcgctcatc tgcgacccaa acggaagaac
     2761 acggcagcaa gacccaacac gacgagaccg acaattgcaa agccgatcca gccccagata
     2821 gttggcgtcg ataccgtgac gcgaaagtcc gccgatttat tcgtctcagg cgaattggcg
     2881 gtcaaggtta acatgtaatc gccggcgatg gttcgctccg gcgcgagaat ctccattttg
     2941 atctgccgta cttcaccggg tccgagcgca tcgatcttat cgggcctgaa ttcgaccgtc
     3001 cacttgtcgc tgggcttctt ggtgacaaaa ttaagatttc ggattggcgc ggttccggca
     3061 ttaccaatga caaaatcgat cggggtctta gtgccggcga tcaccgacgt attaagtgtg
     3121 ccggcgaggg agcccatctt gaggtcctgg gtccccttga gcgatacttt gacattcgct
     3181 gcggcttcga aggctccggc gcgggcttga atggtgatcg gatactcctt ggcttcggca
     3241 ctggctgggg tatcgatctc gacgctcaaa gtctccgagc cgttttcttt cagctgaatc
     3301 gaggagatct gctgatcacc aaactgcggt ttgaagcgca ccgtccagcc ttgtggcgcc
     3361 tgcgcgacga tatttgtcgg cagcggcttg ttcgtttcat tcttcagatc gatggtaaac
     3421 tttaatgtct gccccgaagc tgaagtgaga accgggtatt ggctgctaat tttaatccca
     3481 cccgtatcga cttttttgga tgtcacccga taattaatcg tctccgtata ctgcgtcgat
     3541 cccttgctat ctttagctgt caccttcacc ggataggtgc caggtttggc gctatcggga
     3601 atcttggcct tgaactcgag cgtggtggac ttgttcgctg tttgatcacc accttgcacc
     3661 atgaccgaac gcacaggaaa gctcggatag cgtgagttga agttcacgtc ccagccggag
     3721 gggacacctt caatggtcaa gctgacctcc accggttctt tggttcgatt gacgacctct
     3781 gcgtccatcg ttgcctcttg gccctgaccc acggtcagat ctttgaacgg aagaatgaga
     3841 ttgaacgatt ccttcgatgc cggagcgtct ttcgacggtg ccggtgtgcc ggtttcagct
     3901 gcattgccgc gcgcactgaa tccgcctgac acgagtaaaa gcaaaaatag caaacacgtc
     3961 cacatccgcg gccgatatgt ggtttcctcg accctcataa cgatcccccg ttcatagaaa
     4021 atggatggtg ataaataacc ataaactttt ttacggtcaa gggcctctca agagctcacg
     4081 ttttgcccaa ctgccgcaat ccctcgtaga gcacgatgga aactgcgttg gataggttca
     4141 gactccgaac gccgctgccc atcatcggta tgcgcaatgc agcatcggga ttggcgtcca
     4201 acaaggcttg aggcaggccg cgtgtttcgg ggccaaacac aagaaaatca ttttcgccgt
     4261 aacgcaccgt ggtataggat ttgacagcgc gtttagaaaa aaacagcatc ctttcaggcc
     4321 gagcattcga taggaacgca tcccagcagc tatgcacgcg aaggtcgacg aactcccagt
     4381 aatccaagcc ggctcgctta aggtgcttgt catcgatctt gaagcctaac ggttcgatca
     4441 ggtggagcgg tgtcaaagtt gcggcacaca gtcgcgcaac gcttccggta ttcggcggga
     4501 tttcgggttc gaacagaacg atgtgcacgg ccagctgatg caactcgaga agatctgctt
     4561 ttgtttccat cttcactgct aacaatgaaa tggtcaaatg taaaagggat taacactccg
     4621 tgcgcgacac ggaagcagcg ctagacagtc gtattgacgc gattcatcag gcggtagtag
     4681 aagagcagag cgctaacagc ggagtcatgg gtca
//
LOCUS       DASULZ010000296         4060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_443810, whole genome shotgun sequence.
ACCESSION   DASULZ010000296 DASULZ010000000
VERSION     DASULZ010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443810"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1260)
                     /locus_tag="VFY96_06205"
     CDS             complement(184..1260)
                     /locus_tag="VFY96_06205"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX5606088.1"
                     /translation="MLILSNEDIEKILPVGACLEVLEEAYRDLGNGMAATVPRYDVFS
                     PTKPGEFYEYKTMSGVLPARNVAALRLNSSVVTWYEKAGGIRKDKLPVAGGDKFVGLV
                     MLFSTETGEPLAIFPDGYVQKLRVAGASAIAARHLARKDATVMALLGAGWQASAHLIT
                     LSMVRDLRQVRLYSPTLESRRRFVENGSGKVAAQLIECASAEEAIETADMVICTTNSI
                     TALFDGEILPPGVHVSCVKPCELDAATYRRADPLIIHWQEAKPFQIAIGVERQSIPDI
                     AEGWQHPLTREDCAIWNFPTLSQLVNKQHPGRTNEQAISCFCNNVGLGLQFAAVGSEV
                     LARAREARVGREIPTEWFLEDVHP"
     gene            complement(1439..2026)
                     /locus_tag="VFY96_06210"
     CDS             complement(1439..2026)
                     /locus_tag="VFY96_06210"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606089.1"
                     /translation="MKKISYLGVIIAFIVLLSGRALAGEPTAQLSSTINEFVTILVNT
                     PVAQLRASGLPDNAMALVLSRFDFSEMTKRSLGSHWQALAGAEQREFVDAFTQKLLRA
                     YGRSVRASGDEKVQYERESREGPLATVETKVVSGSGDELPINYQLHNVDGQWKVYDMV
                     IDHVSIVSNYRAQFERVIAKSSVKELLQRIKQQDS"
     gene            2181..2627
                     /locus_tag="VFY96_06215"
     CDS             2181..2627
                     /locus_tag="VFY96_06215"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEX5606090.1"
                     /translation="MHYFAYGSNMNRAQMQRRCPSSNFVCVARLADYQFAITRHSRLR
                     DCGTANVIPGPGKEVWGVVYEVSDSELLLLDAFEDGYRRAIMPVYALGDGVHPFDALI
                     YIAEIEPNVPLPNSEYKRLIIEGAKQWQLPGVYIATLESLQAAVDG"
     gene            2892..>4060
                     /locus_tag="VFY96_06220"
     CDS             2892..>4060
                     /locus_tag="VFY96_06220"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEX5606091.1"
                     /translation="MAISLSDVRVKANGVARHPRTRKIAIWLISVFVAIGVLFGLVAP
                     PLIRGKIAAALSDKLHRQVSIEQIRLNPYAMTATIRGFLIKERQSQQPAVSFDELYVN
                     LQLQSLFRLAPVIKDLRLVKPYINVVRNQARKYNFQDLIDEFTSGPPGPSGPPARFAL
                     NNIQVIDGRIDFDDQPEKTKHTITQIRIGVPFISSLPSYTDINVKPEFSAVINGAPFR
                     LDGATMPFKSSHDSTLEIDVEKLTIAKYLEYSPVELKFKVPSGRLQANLKASFKTSKE
                     NPPALSISGNVGLVELDMQQMDDTPLLKLPKFDVTIDNIQVFNNRTALKSIRSEGLEL
                     HLKRNSEGQLNVANLVEVQNKPSTTEAAKSAQPYGFAVEEIALGSSTIHIEDQRP"
BASE COUNT          940 a         1167 c         1030 g          923 t
ORIGIN      
        1 catctaaacc gtaattttcg aagagcttgg cttccttcgc gacccatagc accgccatgg
       61 atcccaccgt cgtcggatag gcgactttaa tcttaccctg accatgtgca agtggcggcg
      121 caaaagcggc gcataaaatt acggccatta gacatttgaa actcttcact cagacctcat
      181 agattatgga tgcacatcct cgagaaacca ctcggtgggg atttcgcggc cgacccgagc
      241 ctccctggcg cgcgccaata cttcactgcc caccgccgcg aactgcaaac cgagtccgac
      301 attattgcaa aaacaactga tcgcctgttc gttcgtgcgt ccgggatgct gtttgttgac
      361 caactgtgaa agagttggaa agttccaaat cgcgcagtcc tcgcgcgtaa gaggatgctg
      421 ccaaccttcg gcgatatccg gaatggactg gcgctcgacg ccgatcgcga tctgaaatgg
      481 tttcgcctct tgccaatgaa tgatcaatgg atcggcgcgc cgataggtgg cagcgtcgag
      541 ctcgcacggc ttaacgcagg aaacatggac cccgggcggc aagatctcgc catcgaaaag
      601 cgccgtaatc gaattggtcg tacaaatcac catatcggcc gtctcgatcg cttcctctgc
      661 actggcgcat tcgattagct gcgcagcaac ttttccggac ccattttcga caaaccgccg
      721 ccgcgattcc agtgttgggc tgtaaagcct gacctgtcgc agatcacgaa ccatcgacaa
      781 tgtgatcaag tgcgcgctcg cttgccaacc cgcgcccagc aatgccatca ccgttgcgtc
      841 tttgcgcgcg agatgacgcg cggcaatcgc gctggcaccc gcgacacgga gcttctggac
      901 gtagccatcg ggaaaaatcg ccagcggctc gcccgtttcg gtgctgaaca acatcactaa
      961 accgacgaat ttgtcgccgc ccgccactgg caatttgtct ttgcggatgc cgccggcctt
     1021 ttcgtaccac gtgacgacgc tcgagtttag ccgcagcgcc gcaacattgc gcgcgggcag
     1081 gacgccgctc atggtcttgt attcataaaa ctcgccgggt ttggtcgggc tgaagacatc
     1141 gtagcgcggc accgtcgcgg ccatgccgtt tccgagatcg cgatacgcct cttcgagaac
     1201 ctcgagacag gcgccgactg gaagaatttt ttcgatatct tcgttcgata agatcaacat
     1261 gctaactctc cttacactcg ctgagtcata ggtcccgcga caaacttttg caaggcgcgg
     1321 caatcagcgg ttaaagctga cggaagggac gcaaagcttg tagtcatgcc ggacagggcc
     1381 ggcgcgttta atagttttgt aaagagcgag acgaagctgg agagaacgtc gaaagtcttc
     1441 aggagtcctg ctgtttgatt ctttggagta gctctttgac ggaagatttg gctatgacgc
     1501 gctcgaactg cgcgcgataa ttgctgacga tactgacatg gtcaatgacc atatcgtaca
     1561 ccttccactg accatccaca ttgtgcagct gataattgat gggaagctcg tcgccgcttc
     1621 cgctgacgac ttttgtctca acggtcgcca gaggaccctc acgagactcg cgctcatatt
     1681 gaactttctc atcacccgaa gcacgtaccg accgtccgta ggcgcgcaat agtttttggg
     1741 taaaggcatc gacaaactca cgctgttcag cgcccgccaa tgcttgccaa tgtgagccga
     1801 gcgagcgctt tgtcatttcc gaaaaatcaa agcggctaag aaccagcgcc attgcgttgt
     1861 ccggcaaccc ggacgcgcgc aattgtgcca cgggagtatt tacgagaatc gtaacgaatt
     1921 cgttgatggt cgagctaagc tgtgccgtcg gctcgcctgc tagagcccgc ccagaaagca
     1981 aaacaataaa cgcgataata acacccaagt aactgatttt tttcatattc tttttcccgt
     2041 tcgataaaat cgtttatatg ttaactttta tgtgagacgc gtgcaatgac gcgaacgatt
     2101 ttcttttcac tgacattgaa gagttgtcga ggtcgcggtt tttttttacc atgcagcttc
     2161 agtgagagga gataccgttc gtgcattatt tcgcctacgg ctcgaacatg aacagggcac
     2221 agatgcagcg gcgctgtccg tcctcaaact ttgtttgtgt tgcgcggttg gctgattacc
     2281 aattcgccat cacgcgccac tcccgtttgc gcgactgcgg cacggccaac gttattcccg
     2341 gtcccggaaa agaggtgtgg ggcgtcgttt atgaggtgag cgactcggag cttctgcttc
     2401 tcgatgcttt cgaggatggc tatcggcgcg cgatcatgcc ggtttatgcg ctcggtgacg
     2461 gcgttcatcc gttcgacgcc ttgatctata tcgccgagat cgaacccaat gttccgctgc
     2521 cgaattccga atacaagagg ttgataattg aaggcgcgaa acagtggcaa ctccccggtg
     2581 tatacatcgc cactctcgag agcttacaag ccgctgtcga tggttagtga tccgcaaaag
     2641 cgctgccctt gcggcaatcc gcacccggtc tgcataacgc gcacgtaatt agaacccttt
     2701 gtacggcgca tggaatgcgc cctctgaatg tctcccatcc ctctcataag ctttcgcggc
     2761 gtgatggcaa acgccattca atgagattgg gcagacccta ttaagcccga tcaattattt
     2821 tctccgctcc tcgtggacat acagcctgcc ccgagctaag atcgcggcaa atctaactac
     2881 cggctattcc aatggcaatc agcttgagcg acgtgcgggt caaggcgaac ggagtcgcac
     2941 gccatccgcg cacacgcaag attgcgattt ggctgatttc agtttttgtc gcgatcggcg
     3001 tcttgttcgg cctcgttgct ccgcctttaa tccgcggcaa aatcgctgcc gcgctttctg
     3061 ataagctcca ccgtcaggtt tccatcgagc agatcagact caatccttat gcgatgaccg
     3121 caaccattcg tggctttctc atcaaggagc gacagagtca gcagccggcg gtctcgtttg
     3181 acgagctata cgtcaacctg cagcttcagt cgctgtttcg cctcgcgcca gtgatcaaag
     3241 atctccggct cgtgaaaccc tacatcaatg tcgttcggaa ccaggcccgc aagtacaatt
     3301 ttcaggacct gatcgatgaa ttcacctccg gtcctcctgg gccgtccggg ccgccggcgc
     3361 ggttcgcgtt gaacaatatc caagtcatcg acggcagaat cgattttgac gaccagccgg
     3421 agaaaacgaa gcacacgatt acccagatca ggatcggcgt gccattcata tcaagcttgc
     3481 cgtcctacac ggacattaac gtaaaaccgg aattctccgc cgtcattaat ggcgcgccgt
     3541 ttcgtttgga cggtgcgacg atgcccttca aaagcagcca cgacagcacc ctcgagatcg
     3601 atgtcgagaa actgacgatc gcaaagtatc tcgagtattc tcctgtcgaa ttgaaattta
     3661 aggttccctc cggacgactg caggctaacc tcaaagccag ctttaaaacc tcgaaagaaa
     3721 atcccccagc gctctctatc agcggcaacg tcgggctagt cgagttggac atgcagcaga
     3781 tggacgacac gcctctgctc aagctcccca agtttgacgt gaccatcgat aatatccagg
     3841 tattcaacaa ccgcactgcg ctcaaatcga tcagatccga aggtctcgaa cttcacctga
     3901 agcgcaactc agagggccaa ctgaacgtcg cgaatttagt ggaagtccag aacaagccta
     3961 gcactacaga agcggcgaaa tccgcgcagc cgtacggctt tgccgtggaa gaaatcgctc
     4021 tcggttcatc gaccattcac attgaagatc agcggccgca
//
LOCUS       DASULZ010000297         2836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1255214, whole genome shotgun sequence.
ACCESSION   DASULZ010000297 DASULZ010000000
VERSION     DASULZ010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255214"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..620)
                     /locus_tag="VFY96_06225"
     CDS             complement(93..620)
                     /locus_tag="VFY96_06225"
                     /inference="COORDINATES: protein motif:HMM:NF025116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX5606092.1"
                     /translation="MADKNHLILTAIGPDQVGLVQRLSEFISRHGCNIEDSKMAVFCG
                     EFAVIVLITGEADNLANIGRNYQQIEKETGLSLAVKTPSSRKTGDSFLPYRLTASCMD
                     HPGIVYQISALLSAHGINIESMETKTYAAPVSGTPIFQLEADLAIPTRTVLRDRFAEI
                     QREENIDIELTSLRA"
     gene            complement(725..2017)
                     /locus_tag="VFY96_06230"
     CDS             complement(725..2017)
                     /locus_tag="VFY96_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606093.1"
                     /translation="MSNRSSWEPPKALSLDEVLQLDRQVASMSDLSIRVSEDIFRIRE
                     LEMDWDIGVMVYEPENTNRIPIGSDGKKIGIFLLHGGVSDFKSVERIARILPGKFAIK
                     VASMTFPGRFYFLDSSRDWPGDVEIGDGSARTPLWTRETQITPDQYEIIHDQSKRKSY
                     GTLVSLWAKPGTEFFHRMAAWPVAFEAAIKQTARRHFPEDEFAVYIHGHSTGGPFAMM
                     ASQRVANIAGIVGYGSSPFGWMYPLISGDDWEFPFNQLRLRTWRDTARYMYEGMKHEN
                     FGLPMLIELTFERWQIAKRRPNFKAEDFVHKNSARALETAARVSAERLKLSNRETDAL
                     VQHYLGYTRELSGPGTKPVPNFLSIHGINDDTVTLGRCQRSLPLFAKMNPAPEVHSIS
                     LGAGIHSWGWTDDKLSQGIAPPVAKMWYDAVINGFFIR"
     gene            complement(2061..>2836)
                     /locus_tag="VFY96_06235"
     CDS             complement(2061..>2836)
                     /locus_tag="VFY96_06235"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX5606094.1"
                     /translation="AEHVWRGYLDPALPGYKPEIAAQLRPFLERVYRNCDDHLALFFE
                     KRPPETILAVISDHGMQGINKRIALNRVLQEGGLLTLDPQGKVDLSKTKVLYPPVNNG
                     YLLINSTDRKNGIVAPEERAQVIEATRELLDSLRDGDRPVITAMYDSESDGAKMGIGG
                     TVGGDLYIDLASGYDFDPRIGPGPLITEAEPYGNHGTDAEQISMRTIMVLNGPGIRRG
                     QKLVNVRIIDFAPTLAWLLHLSKPKNATGRVLNEALEQP"
BASE COUNT          614 a          757 c          803 g          662 t
ORIGIN      
        1 atcggagttc tgaattatta gcgggctttc actcaacgct ggtttcgcga gccaggcgtt
       61 tagttcgagc gaagctcgac tcattttggg tgttaagcgc gcaacgaagt gagctcgatg
      121 tcgatatttt cttctcgttg aatttccgca aagcggtcgc gcaatacggt gcgtgtggga
      181 atggcgaggt cagcttccag ctgaaagatc ggcgtgccgc tgaccggtgc ggcataagtc
      241 ttggtttcca tcgattcgat attgatgcca tgggcgctta gcagggcgct gatttggtaa
      301 acaatgccag ggtgatccat gcaggaggcg gtgagccggt aagggagaaa ggaatccccg
      361 gtcttgcggc tcgagggcgt cttcacggca agcgataaac cggtttcctt ttcgatctgc
      421 tgataattgc gaccgatgtt ggcgagattg tcggcttcgc ccgtgatcag cacgataacc
      481 gcgaactcac cgcaaaaaac cgccattttg ctgtcttcga tattacagcc gtggcgcgag
      541 ataaattcgg agaggcgttg aaccaatccg acttgatccg gcccaatcgc ggtgagaatc
      601 agatgatttt tgtccgccat ggcgctaccc taagaaattg gttcagagag ttcaatcgtt
      661 aaagccattc ccaacatccc atgcaaaact ccagcgctgc atttcgcaac ctgacgcaat
      721 gccgctaccg tataaaaaat ccattgatga cggcgtcgta ccacatcttt gccactggcg
      781 gagcgatgcc ttgcgataat ttgtcatcgg tccagcccca ggaatggatt cccgcgccaa
      841 gtgaaatgct gtgcacttcg ggcgcgggat tcatttttgc gaagagaggc aacgacctct
      901 ggcaacggcc gagggttacg gtgtcgtcgt tgataccgtg gatcgagaga aaattgggca
      961 ccggttttgt gcctgggccg ctgagctcgc gagtatagcc gaggtaatgc tggaccagcg
     1021 cgtcagtctc tcggttcgag agcttcaacc gttcggcaga gactcgggcg gcggtctcca
     1081 gggctcgagc ggaattttta tggacgaagt cttcggcctt gaaattgggc cgtctcttcg
     1141 cgatctgcca gcgttcgaag gtgagttcga taagcatcgg cagaccgaaa ttctcgtgct
     1201 tcatgccttc gtacatgtac ctcgcggtgt cgcgccaagt gcgcaggcgc aattggttga
     1261 agggaaactc ccagtcatcg ccgctgatca acggatacat ccaaccgaac ggcgagctgc
     1321 cgtagcccac tatccccgcg atattggcca ctcgttgaga cgccatcatc gcaaacggcc
     1381 cgccggtgga gtggccatga atatagacgg cgaattcatc ctcgggaaaa tggcggcgag
     1441 cggtttgctt gatcgctgcc tcgaaggcaa ccggccatgc ggccatgcga tggaagaact
     1501 cggttcccgg tttggcccaa agcgaaacaa gcgtgccgta actttttcgt ttagattgat
     1561 catgaatgat ctcgtactga tccggcgtaa tctgagtctc tctggtccaa agcggggtgc
     1621 gcgcggagcc atcgccgatt tccacatcgc ccggccaatc tcgactcgaa tcgagaaaat
     1681 agaagcggcc tgggaacgtc atgctggcaa ctttaatcgc aaatttgccg ggcagaatgc
     1741 gcgcgatccg ttcgacggat ttgaaatccg aaacgccgcc gtgaagtaag aagatcccga
     1801 tctttttgcc atctgacccg atggggattc tattggtgtt ttcgggttcg tacaccatca
     1861 cgccgatgtc ccaatccatc tctagctctc gaatgcggaa aatgtcctca gaaacacgaa
     1921 tcgacaggtc agacatggac gcgacctggc ggtcgagctg tagaacttca tccaacgaaa
     1981 gcgctttggg tggctcccat gacgatcggt tgctcacgag tgatctcctc agaatattgg
     2041 cacttgtgcc gtacctcttg tcacggctgc tcgagggctt cattcaatac gcggccggtg
     2101 gcattcttcg gcttagatag gtgaagcagc cacgcgaggg tcggcgcgaa atctataatg
     2161 cgcacgttga cgagcttctg tccgcggcgg atgcccggcc cattcaaaac catgattgtg
     2221 cgcatcgaaa tttgctccgc gtccgtgccg tgattgccgt acggttcggc ttcggtgatc
     2281 agtggaccgg gcccgatgcg cggatcgaag tcatagccgg acgcgagatc aatatagagg
     2341 tcaccgccca ccgtgccgcc aatgcccatt ttggctccat cagattcgga gtcatacatt
     2401 gcggtgatta caggtcgatc tccgtcgcgc agtgaatcga gcagttcacg cgttgcctca
     2461 atcacctggg cgcgctcctc cggtgcgacg ataccattct tccgatcggt ggaattgatc
     2521 aagagataac cattgttgac tggcggatag agcaccttgg tttttgacag gtcaactttt
     2581 ccttgagggt ctagtgtcaa aagcccgcct tcttgcagca cccggttcaa tgcgatccgt
     2641 ttattgattc cttgcatgcc gtggtcagaa atcacggcga gaattgtttc tggcggacgt
     2701 ttttcaaaga atagagccag gtggtcgtca cagtttcgat aaacgcgctc caagaacggc
     2761 cgcagctggg ccgctatctc cggcttgtag ccagggaggg ccggatcgag atagccgcgc
     2821 cagacatgct ccgcct
//
LOCUS       DASULZ010000298         2439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_240324, whole genome shotgun sequence.
ACCESSION   DASULZ010000298 DASULZ010000000
VERSION     DASULZ010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240324"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VFY96_06240"
     CDS             <1..321
                     /locus_tag="VFY96_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX5606095.1"
                     /translation="YGSIDIDSNAQICRFLTTRAPSPPSGPPHKLMFSGVQILEPTIF
                     EYMAGMPQKFSTTRETYPKMLAAGEALYGFHFDGFWQDLGTAERIKQAEAKLSGGEAS
                     LHYL"
     gene            complement(324..845)
                     /locus_tag="VFY96_06245"
     CDS             complement(324..845)
                     /locus_tag="VFY96_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017382018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEX5606096.1"
                     /translation="MNQQRLIVLGLAFVMGCSMAASGQRATAELKDKDGKTVGSASLR
                     EQPDGVLVRVETKGLTPGLHAVHVHAVGKCEGPGFATAGGHFNPTQKKHGFKNAEGVH
                     AGDLPNMLAAKDGSGRFEALTDAITLRAGSMSVFDSDGSAIVIHATSDDYMTDPAGNS
                     GDRVACGVIVPNG"
     gene            996..>2439
                     /locus_tag="VFY96_06250"
     CDS             996..>2439
                     /locus_tag="VFY96_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606097.1"
                     /translation="MSKARRHKTRPNASKLRAARHEQIRFEWNQKFSLVLVGIISGIH
                     FCALAALFSPASGIFNTQPLVDQDWGLHFHHLKSLAAFWQQDKITWGYNPFFMAGYPS
                     NTIQDLSIKFFEWTALALSAITLSPVQWFKICAFLAMASVPWLLYMAASNLFFADESK
                     HFIAALAAFFGAAYWWNSLPREMFFYGMIGFPAAAYLSIWGIALFYRLALFPTKIGLT
                     HFAWLVFAAAAIPLHVQSVITFLPPMFALLLADKRLITGRLIAWSAGAAALALFVNSA
                     WLIPAIQHRADDISATIVDELPLFVGTSPFTFLLDYLGPRGFWTFRSSVLEKGFRLML
                     LILGTVGAWHLLRTRQRLLGVMLATAALVLFAVTYFGALASVLKAWQPLRFKVPLDLI
                     LAIAASYCLSVSLARGGSTQRFVGATVTLGLLAFLLNLVQTESAGRLQLRSTFSPELK
                     AIVEWINEETPANGRLLFEESGDESGFVYDG"
BASE COUNT          511 a          679 c          627 g          622 t
ORIGIN      
        1 tatggttcga tcgacatcga ctcgaacgcg caaatttgcc ggttcctcac gacccgggcg
       61 ccgagtccgc catcggggcc acctcacaag ctgatgtttt ccggagtgca gattttagaa
      121 ccgaccattt ttgaatatat ggccggcatg ccacaaaagt tcagcacaac cagggaaacg
      181 tatccaaaga tgctcgccgc cggcgaagcg ctttacggat ttcacttcga cggtttttgg
      241 caggatctcg gaacggcgga gcggatcaag caagctgaag caaagctctc gggcggcgaa
      301 gcgagcttgc attatcttta gcgttaccca tttggcacaa tcaccccaca agcgacgcga
      361 tcacccgaat ttcccgcggg atcggtcata taatcgtcgc ttgtcgcatg aatgacgatg
      421 gcgctgccgt cgctatcaaa caccgacatc gatcccgctc gcaacgtgat cgcatcggtc
      481 agagcctcga aacgaccgga gccatccttg gccgccagca tattcggtaa atcgccggca
      541 tgtactcctt ccgcattttt gaagccgtgt tttttttgcg tcggattgaa atgcccgcca
      601 gcagttgcga acccaggacc ttcgcacttg ccaacggcat gcacgtgcac ggcgtgcaaa
      661 ccgggggtta gacctttggt ctccacacgc acgagcacac catccggttg ttcacgaagg
      721 ctcgcgcttc cgacggtttt cccgtccttg tctttgagct cggccgtcgc gcgctgtccc
      781 gatgccgcca tcgaacagcc catcacaaaa gctaaaccta aaacaatcaa ccgttgctgg
      841 ttcatgatga ttgcaaatct cctttgcgcc taattagcac gggcatacga gagtagcaat
      901 tcgccgaaaa ttcttagcga agcgaccggc gttcgcgaga agcttccgat caattgaccg
      961 acctggtatc ggtcataaat cggagaagac tgcggatgag caaagctcga agacacaaga
     1021 ctcgaccgaa tgcttccaag cttcgagccg cgcgccatga gcaaatccga ttcgagtgga
     1081 atcaaaaatt cagcctggtc ctggtcggta tcatctcggg cattcatttc tgcgctctag
     1141 ccgcactctt tagtcccgcc tcgggaatct tcaatacgca gccgcttgtt gaccaggatt
     1201 ggggccttca ctttcatcat ctcaagtctt tggccgcgtt ctggcagcag gacaaaatca
     1261 cctggggata caaccctttt ttcatggcgg gttatccttc caatacgatc caggatctca
     1321 gcatcaaatt ttttgaatgg accgcattgg cgctgtccgc gatcacctta tcacctgtcc
     1381 agtggtttaa gatctgcgcg tttctcgcga tggcgagtgt tccttggctc ctgtacatgg
     1441 ctgccagcaa tttgtttttc gcggacgaga gcaaacattt catcgcagcg ttggcggcct
     1501 ttttcggcgc ggcgtattgg tggaactcat tgccgcggga aatgtttttt tacggtatga
     1561 tcggttttcc agcggcagcc tatttgagca tctggggaat agctcttttt taccgactcg
     1621 cgttatttcc gaccaaaatt ggcttgacgc atttcgcatg gctggttttt gcagctgccg
     1681 caataccgct gcatgtgcaa tcggtcatca cttttttgcc gccgatgttc gccttgctct
     1741 tggctgacaa gcgtttaatc accggccggt taatcgcatg gagtgccggc gccgcggcgc
     1801 ttgcgttatt cgtcaattca gcatggctga tcccagcgat ccagcatcga gcggacgata
     1861 tctcagccac gatcgtcgac gaattaccgt tgttcgtcgg aaccagtccg tttacttttc
     1921 tactcgatta tcttggacca cgaggatttt ggacatttcg ctcgtctgtt ctggaaaaag
     1981 gtttccgcct aatgcttctc attctcggca cggtgggagc ttggcatttg ctacggactc
     2041 gccaacgtct gctcggagta atgctagcaa ccgcggccct ggttcttttc gcagtgacat
     2101 atttcggtgc gcttgcgtcg gtgcttaaag cctggcagcc gttgcgcttt aaagtgccgc
     2161 tcgatctaat tctcgccatc gcggcgtcct actgtctcag tgtctcttta gcacgaggcg
     2221 ggtcaacgca acggttcgtc ggtgctacgg tgacattagg ccttttggcg ttcttattga
     2281 atctggttca gacggagtca gccggcagac tgcagcttcg ctcgacattt agtcctgagc
     2341 tcaaggcgat cgtcgaatgg ataaatgagg aaacaccagc aaatggccgg cttctgttcg
     2401 aagagtccgg cgatgaaagc ggtttcgtct acgatggaa
//
LOCUS       DASULZ010000299         2371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_951404, whole genome shotgun sequence.
ACCESSION   DASULZ010000299 DASULZ010000000
VERSION     DASULZ010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951404"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VFY96_06255"
     CDS             <1..744
                     /locus_tag="VFY96_06255"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5606098.1"
                     /translation="TRQDLVDFYKLYYAPNNAFIVVVGDFSPDEILPKIRTAFGKIPQ
                     HAEPPKVSVQEPEQRGERRVILKKEAELPFVLLFYHAPNLQSADSFALDVLSVVLAGG
                     RSSRLYHELVYEKRLVRGVDADYSRVSVDPMGFSITAQLLPGIPLANVEREIYGSLEK
                     IKSELITDRELQKAKNQIEAAFIFDQDSIFGQAMKIGYYEITGEWRQLDAYLDGIRKV
                     TREDIRRVTRQYLDTDRRTVGTLIPTKEQ"
     gene            813..2063
                     /locus_tag="VFY96_06260"
     CDS             813..2063
                     /locus_tag="VFY96_06260"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5606099.1"
                     /translation="MTPKRTVLGNGMVLLTSEQRTLPMVSIELFVNAGSSHESAPQAG
                     LANLTAKLLTYGTKRRSAVQINETLDFIGASLETACGQDTASLSMTVLKKDLATGLEL
                     LADVLTEPTFPQPEIDRQKQAIIATIRATEENPGAVAGKAFAAALFPQSPYGRPVEGT
                     EASVKTLQQKNLQDFFARYYRPNGAIIAVVGDVSEQEIASLLNTALRSWAKGEPSAVV
                     TVPVKIGPAKRARVNKELTQANIVMGHSGVTRDNPDYYAIQVMNYVLGGGGFSSRAMD
                     SIRNERGLAYSVYSFFAAEKSHGTFEFVMQTKNETAMEAIRIATDEIRRMREEPISEQ
                     ELNDAKDYLIGSFPLRFDTNRKVASFLAQVEYFKLGLDYPERYPDLIRSVNREEVLRV
                     AKQYLHPETLITVIVGNLKKIAEK"
     gene            2060..>2371
                     /locus_tag="VFY96_06265"
     CDS             2060..>2371
                     /locus_tag="VFY96_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5606100.1"
                     /translation="MSQSPAVATVSLTPLALRSAACPDLTGGSVLGIVGISSRVMETE
                     FAERVFMQKVLIIEDHADMRELLIWQVELMGYTPISAKRGRDGVEKALSEKPKLIIMD
                     IM"
BASE COUNT          562 a          616 c          684 g          509 t
ORIGIN      
        1 acgcgccagg atctggtcga tttctacaaa ctctactatg cgcccaacaa tgcattcatc
       61 gtggtcgtcg gcgatttctc gccggatgag attttaccga agattcgcac cgcgtttgga
      121 aaaattccgc agcacgctga accaccgaaa gtgagcgtac aagagccgga acagcgcggc
      181 gagcggcgcg tgattctcaa aaaagaagcc gagctaccat ttgttctgtt gttctatcat
      241 gcgcccaatc tgcaaagcgc cgacagcttt gcgctcgacg tactctcggt ggtcctcgcc
      301 ggcggccgga gctctcggct ctatcacgag ctggtctacg aaaaacggct ggtgcgcggc
      361 gtcgatgcgg attatagccg tgtctccgtc gatcccatgg ggttctccat taccgcacag
      421 cttctcccgg gaattccgct ggcaaatgtg gaacgggaga tttacggatc gctggagaag
      481 atcaaatcag agctgatcac tgaccgggaa ctgcaaaaag ccaaaaatca aatcgaagct
      541 gctttcattt tcgatcagga ttccatcttt ggacaggcga tgaaaatcgg ttattacgaa
      601 atcaccggag aatggcgcca gctggacgcc tatctagacg ggatccgaaa ggtgacgcgc
      661 gaggatattc gccgggtgac gcggcaatat ctcgataccg atcggcgcac ggtcggcacg
      721 ctgattccca cgaaagagca atgagctggt tctgcttccg gctgttgccg tgtttggctt
      781 ttttctgtgg agcaactttg agcgaggcgg gaatgacgcc caaacgcacg gtcctcggca
      841 atggcatggt gttgttgaca tcggagcagc gcaccttgcc gatggtgtcc attgaattgt
      901 ttgtaaacgc ggggtctagc catgagagtg cgccgcaggc cggtttggcc aatttgacgg
      961 caaagctgtt gacctacggg accaagcgcc gaagcgcggt tcaaattaac gaaaccctgg
     1021 attttatcgg cgcgagtctt gaaacggctt gcggccaaga tacggcaagc ttgagcatga
     1081 ccgtgctcaa gaaagacttg gcgacaggac tcgagttgtt ggccgatgtt ctgactgaac
     1141 ccacttttcc tcaacccgag atcgatcggc aaaaacaggc gatcatcgca accatcagag
     1201 cgaccgaaga aaatcccggc gccgtggcgg gaaaagcttt tgctgcggca ctttttccgc
     1261 agagtccata cggccgcccg gtggaaggca ctgaagcaag cgttaagaca ctgcagcaaa
     1321 aaaatttgca ggattttttc gcgcgctact atcgccccaa cggcgcgatc atcgcggtgg
     1381 tcggtgatgt ttcagagcag gaaatcgcat ccttgctcaa tacggccctg cggagctggg
     1441 ccaagggtga gccgagcgcc gtcgtgacgg taccggtgaa aatcgggcca gcgaagcgcg
     1501 cccgggtgaa caaggagctc acccaggcca atatcgtgat gggccacagc ggtgtcacgc
     1561 gggataatcc ggattattat gccatccaag tcatgaacta cgttttgggc ggcggtggtt
     1621 tttcctctcg cgccatggac tcgattcgca atgaacgagg gctcgcgtat tcggtctata
     1681 gtttttttgc cgcggaaaag agccacggca cgtttgaatt cgtcatgcag accaaaaatg
     1741 aaactgcgat ggaagcgatt cgtatcgcta ctgacgaaat acggcggatg cgcgaggaac
     1801 cgatcagcga acaggagctt aacgacgcca aagactacct gatcggcagc ttcccgcttc
     1861 gcttcgatac gaaccgcaag gtggcgagtt ttctcgctca ggtggaatat ttcaagctcg
     1921 ggctcgatta tccagagcgc tatccggatt tgatccggag tgtcaatcgc gaagaagttt
     1981 tgcgggtggc aaaacagtat ttgcatcctg aaacgctgat cacggtcatt gttggaaacc
     2041 tgaagaagat cgcggaaaaa tgagtcagtc acccgccgtc gcaaccgtca gtctcacgcc
     2101 tttggcgctg cgatctgccg catgtcctga cttgacaggc ggcagcgtgc tcggcatagt
     2161 gggcatttcg tctcgcgtca tggagacaga gtttgccgag cgggtattca tgcagaaggt
     2221 gttgatcatc gaagaccatg ccgacatgcg agaactgctt atatggcagg tcgagctcat
     2281 gggctatacg ccgatctccg cgaagcgcgg cagggatgga gtcgaaaaag cgctcagcga
     2341 aaagcctaag ttgatcatca tggacatcat g
//
LOCUS       DASULZ010000300         3658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_39010, whole genome shotgun sequence.
ACCESSION   DASULZ010000300 DASULZ010000000
VERSION     DASULZ010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39010"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="VFY96_06270"
     CDS             complement(<1..811)
                     /locus_tag="VFY96_06270"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5606101.1"
                     /translation="MNNSDLIARALEQAGVGWVFGVPSGPVLPLIEALRRTSVEFVLT
                     ASETSAGFMASAVGYLTGIPGVCVSTVGPGATNLTTGVGCAWLDRLPLLALTCNVPSA
                     WLERRIQMRIDHHELFRPLSKASISLCRDSVVKSIERAMTLARQEPPGPVHVDLPEDV
                     ALADATECSKAESSPAGVQDRSSEVVEIVQDSLRLSRRPLVMTGLTFTRSHAAHELLK
                     FIERQGLPFATTMHAKGFLPESHRNAVGVIGRARRTDVKRFIDRADLIIAIG"
     gene            complement(896..1696)
                     /locus_tag="VFY96_06275"
     CDS             complement(896..1696)
                     /locus_tag="VFY96_06275"
                     /inference="COORDINATES: protein motif:HMM:NF009825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HEX5606102.1"
                     /translation="MTQIGIVGCGAIGKALIQAAQSGNLSAPIAGVTSRSERTARDFL
                     ASLKNPPPYRSLEELIGRADLLVEAAGGGIVAELAKKVFAARKALMVISVGALLDHPE
                     IIEQSRQSGCRLYVPSGAIAGLDGIKSASVGRIEHVTHTTRKPPAGLEGAPYLTEHGI
                     SLAGLEHEKEIFYGSAREACRGFPANVNVTAAVSLAGIGADQTRVRILAVPGLVRNCH
                     DIDVEGEFGKLHVHIENVPSENPKTGKLTALSIIRALQDALDTVRIGN"
     gene            complement(1741..2169)
                     /locus_tag="VFY96_06280"
     CDS             complement(1741..2169)
                     /locus_tag="VFY96_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HEX5606103.1"
                     /translation="MISVSDLPSLNAALNSLSAVFLFSGFLFIRSGKRESHRACMLIA
                     FSCSILFLISYLVYHYQVGSVPFQGQGWVRPLYFTILLSHTILAVAVVPLALTTLRRA
                     LGENFSAHRRIARWTFPVWLYVSVTGVIVYWMLYHLVPSR"
     gene            complement(2166..2444)
                     /locus_tag="VFY96_06285"
     CDS             complement(2166..2444)
                     /locus_tag="VFY96_06285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEX5606104.1"
                     /translation="MTSTAHSTSAYIKVFYVLLVLTIVEVAIVYLGLPKMLLAALLVI
                     FAVWKASLVAMHFMHLKFEKRTLAVVALTPFVLCVFLILMLMPDIFPH"
     gene            complement(2466..3074)
                     /locus_tag="VFY96_06290"
     CDS             complement(2466..3074)
                     /locus_tag="VFY96_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX5606105.1"
                     /translation="MSEAAVMHVGAEPQETPLTPESWGKLGMWLFLAGDAMSFGCLIV
                     GYGILRHASSSWPIPKDVLGINLTAFMTFLLIVSSVWMVLALAAIQEGNKARFNKFLG
                     LTILGGVMFLGCQAYEWTHLLTHRLPEAGIAFSTSLFATTFFILTGFHGMHVTGGVIY
                     NTCVLAAVNRGRYEAKHVEIAGLYWHFVDLVWILIFTFVYLI"
     gene            complement(3071..3355)
                     /locus_tag="VFY96_06295"
     CDS             complement(3071..3355)
                     /locus_tag="VFY96_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606106.1"
                     /translation="MMKKILSIAAGSGATVFSSPVLTWACAVCFTGTGDAVTDGFNAS
                     VLFLMVTPYAVVGAIVGGLVFTYRRALRKRVQSDSEAIDHFAWIQEDSLR"
     gene            complement(3406..>3658)
                     /locus_tag="VFY96_06300"
     CDS             complement(3406..>3658)
                     /locus_tag="VFY96_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX5606107.1"
                     /translation="EWFRLLHFGLTLSSSIYGGLFYTIIGCHALHVFAALIWLLVVWQ
                     RARAGKYTRQNYVGLQTCSMYWVFVVGLWPLLYGLVYLY"
BASE COUNT          925 a         1090 c          962 g          681 t
ORIGIN      
        1 atccgatcgc aatgatcaga tccgcgcgat cgatgaagcg ctttacatct gtccgccgtg
       61 ctcgcccgat cacaccgacg gcgttacggt gactttccgg caaaaaaccc ttcgcgtgca
      121 ttgtcgtagc aaaaggcaat ccttggcgct cgatgaactt cagcagctca tgcgccgcat
      181 ggctgcgcgt aaaggtcagc ccggtcatca ccagcggtct gcgcgagagc ctcagcgagt
      241 cctgcacgat ctctacgacc tccgacgagc ggtcttgaac ccccgccggg ctgctttcgg
      301 ccttgctgca ttcggtagcg tcagccagcg ccacatcttc cggcaaatcg acatgcaccg
      361 gcccgggcgg ttcttgacga gccaacgtca tggctctctc gatcgactta accacagagt
      421 cacggcacaa gctgatcgac gccttgctca aaggtctaaa tagctcgtgg tgatcgatgc
      481 gcatctgaat gcgccgctcg agccaagcgc ttggaacgtt gcaggtcaac gcaagcaacg
      541 gcaatcggtc cagccaagcg cacccgacac cggtggttaa gttggtggcg cctggaccga
      601 ctgtggatac gcacacaccg ggaatgccgg tcagatatcc cacagcgcta gccatgaatc
      661 cggccgaagt ctcgctggcg gtaaggacaa actctacgga ggttcgccgc agcgcttcga
      721 tcagcggcaa taccggtccg ctgggaacgc caaagaccca acctacgccg gcttgctcca
      781 acgcgcgtgc gatcagatcg ctgttgttca aggattgaat cctcgtcgag agaagaccga
      841 ggatggaaga gagagaatgg aacgatcttc tatcttcgat tctctatccg ccacttcaat
      901 tgccgatgcg cactgtatcg agggcgtctt gcagggcgcg gatgatcgac agagcagtga
      961 gcttgccggt tttgggattt tcactcggaa cgttttcgat atgcacgtgc agcttaccga
     1021 actccccttc aacgtcgata tcgtgacagt tgcgcaccaa acccggcacg gcgagaatcc
     1081 gaacccgggt ttgatcggcg ccgattcccg ccaagctcac ggctgccgtc acattgacat
     1141 tcgcggggaa accgcggcaa gcctcacgtg ccgagccata aaagatctct ttttcgtgct
     1201 ccaaaccggc cagcgaaatg ccatgctcgg tcaagtaagg cgctccctcc agtcccgccg
     1261 gaggctttcg cgtagtatgc gtcacatgct cgatccggcc gacggacgcg gatttaatac
     1321 catccaaccc ggcgatcgca ccggacggaa cgtacagacg gcagccgctc tggcgggatt
     1381 gctcgatgat ctccggatgg tcgagcaacg ctccgacgct gatgaccata agcgcttttc
     1441 gtgccgcgaa gacttttttt gccagctcag cgacgatccc cccgcccgcc gcttcgacca
     1501 ataaatccgc gcgaccaatc agctcttcga gagatcgata gggtggcggg tttttcaagg
     1561 aggcgagaaa gtcgcgcgca gtccgttcgc tccggctggt caccccggct atgggcgccg
     1621 aaagattgcc gctctgcgcg gcttgaatga gcgctttgcc gatcgctcca cagccgacga
     1681 taccaatctg cgtcatcgaa gttctcccgc tctcaggtct atcgtgaacg gtttttccgg
     1741 tcaacgcgag ggcactagat ggtaaagcat ccagtaaaca atcactccgg tgactgaaac
     1801 gtaaagccag acgggaaaag tccagcgagc gatgcgccga tgcgccgaga aattctcgcc
     1861 gagcgcgcgg cgcaacgtgg tcagcgccaa tggaaccacc gcgacagcga gaattgtgtg
     1921 gctcaacagg atcgtaaaat atagcggccg cacccaacct tgcccttgga acggcaccga
     1981 acccacctgg taatggtaga cgagataaga aatcaaaaac aggatcgaac aactgaaggc
     2041 gatcagcatg caagcccgat ggctctcgcg cttgcccgat ctgataaaca gaaaaccact
     2101 aaaaagaaaa acggcgctga gactgttcag cgccgcgttc aggcttggca gatcggagac
     2161 agaaatcaat gaggaaagat gtccggcatc agcatgagaa tcagaaaaac gcacagaaca
     2221 aacggcgtca acgccaccac cgcgagagtt cgcttctcga attttagatg catgaaatgc
     2281 atggccacca aactggcctt ccacacggca aaaatcacca gcagcgccgc gaggagcatt
     2341 ttcggcaacc cgagataaac gatcgcgact tcgacgatcg tcagcacgag gagaacgtag
     2401 aacaccttga tataggcgga tgtggaatga gcagttgagg tcatctctgt cccttacggc
     2461 ttacgttata tgaggtagac aaacgtgaaa atcaagatcc agacgagatc gacaaagtgc
     2521 cagtacaatc ccgcgatctc tacatgctta gcttcatagc ggccgcgatt gacggcggcg
     2581 agcacgcaag tgttgtaaat cacgcctcca gtcacatgca taccatggaa cccggtcaga
     2641 atgaaaaacg tggttgcgaa cagactggtg gagaacgcga ttcccgcttc cggcaagcgg
     2701 tgggtcagca aatgcgtcca ttcataggcc tggcagccca agaacatgac gccacccaaa
     2761 atcgttaggc cgagaaattt attgaacctg gccttgtttc cttcctgaat cgccgccaac
     2821 gccaaaacca tccagacgct gctaacgatc aggagaaacg tcatgaaggc ggtcagatta
     2881 atgccgagaa cgtctttggg tatcggccaa ctactgctcg catggcgcaa aattccgtag
     2941 ccgacaatca aacagccgaa ggacatagcg tcgccggcaa gaaacaacca catgccgagc
     3001 ttgccccagc tctcgggggt caagggcgtc tcttgcggct ccgccccgac gtgcatcacc
     3061 gccgcctcgc tcatctcaag ctatcctcct gaatccatgc gaaatgatcg atcgcttcag
     3121 agtcggactg cacgcgcttt cgtaacgcgc gccggtacgt gaaaactaat ccgccgacga
     3181 tggcgccgac tacggcatag ggtgtcacca tcagaaacaa cacgctcgcg ttaaaaccat
     3241 ccgtgaccgc atcgccggtt ccggtaaaac agacggcaca cgcccatgtg aggaccgggc
     3301 tgctgaacac cgtcgctccc gatccagccg caattgacaa gattttcttc atcataaaat
     3361 catccgcaaa gtaacgtaac gaatttaggc tgtcaaccaa ctgacttagt aaagataaac
     3421 cagaccgtag agcagcggcc aaaggcccac aacaaacacc cagtacatgc tgcaagtctg
     3481 caggccgaca tagttctggc gcgtatattt tcccgctcgt gcccgttgcc acacgacgag
     3541 caaccaaatc aaagcggcga aaacatgaag cgcgtgacag ccgataatcg tgtaaaagag
     3601 cccgccgtaa atgctgctcg ataacgtcaa cccgaaatga agcaagcgga accattca
//
LOCUS       DASULZ010000301         3294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_951816, whole genome shotgun sequence.
ACCESSION   DASULZ010000301 DASULZ010000000
VERSION     DASULZ010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951816"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VFY96_06305"
     CDS             complement(<1..753)
                     /locus_tag="VFY96_06305"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5606108.1"
                     /translation="MYEKHFRLRELPFNLTPDPRFLYTHASCQEAFATLCYGVENRKG
                     IVLITGAAGTGKTTLIKLFMERAAQPVRAACILDPHLTFIELLQCALDAFGVTQLSAD
                     RVSLMQQFRDYLLEQRNHDHRVALLLDESQDIQDEVLEELRLVSDLEHAGDKLVQVVL
                     AGQSEFERRLEQPRFDQLRQRVILRSRLEPLGEEEIGPYIDFRLRTAGYSERSLFQRA
                     AIHRIGYFSQGIPRLINVICDHALLIACATNSE"
     gene            complement(814..2346)
                     /locus_tag="VFY96_06310"
     CDS             complement(814..2346)
                     /locus_tag="VFY96_06310"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5606109.1"
                     /translation="MRIPATYSGSTTLEISGVRDPASRDDLFCPAAPTLLVLDGDHQI
                     REQLERLYLTSGYSVVGLASAADCMRRLTEDDIDFIIASIHLPDADALELIAQIHQRY
                     PALPVVAITSTADIQSTVDVLKLGACDFVVKPFDPAAVLESTRAALENSKPGIQARQL
                     RRWLKGHFEFGDILSPAPQMRRVFELICTAAPIDMPVMIAGEAGTVKEQVAFAIHHHS
                     ARRSAPFIAVNCAGYPDALLQAELFGYEKDAFAGIYDSKPGKIAQAHGGTLFLDEIEV
                     LSLALQKKLLEVIDHRKISRLGASQASHIDVRIIAATKLALKERAPGRVLRDELLARL
                     SAVPIDLAPLRERAIDIPHLVEHFLRYHPVAKSKRIAAVADKVLGQLMRYSWPGNVRE
                     LHNALECAILLAPGRVIEDVKLPESFEDGAAEKSNIAASSLRQWLREKEKYYLSRKLE
                     DLGGNISLTAKSCRIGVRTLSRKMRTYGLDKKIFKEKPLGEKPAAPPRERTVPAAKSG
                     PA"
     gene            complement(2875..>3294)
                     /locus_tag="VFY96_06315"
     CDS             complement(2875..>3294)
                     /locus_tag="VFY96_06315"
                     /inference="COORDINATES: protein motif:HMM:NF015665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HEX5606110.1"
                     /translation="ELAGHINSQMPAFVIRGMADVLNEKRKCLNGAKVLALGVAYKRD
                     TNDVRESPALQVLLGLQEKGCEICFSDPYVPQITVNETNLDSVTLSPELLQSMDLVVI
                     LTDHSGFDYAMITKFSPLIFDTRNALSDLAQPNVYKL"
BASE COUNT          771 a          895 c          861 g          767 t
ORIGIN      
        1 ttcgctattg gtcgcacacg cgatcaatag cgcgtggtcg caaataacgt tgatcaaccg
       61 cggaattccc tgggaaaaat agccgatccg gtggatcgcc gcacgctgaa aaagtgacct
      121 ttcggaatat cccgcggttc gaagcctaaa atcaatatac ggcccgatct cctcttcccc
      181 cagaggctca agacgggacc gcaagataac acgttgccgc agttgatcaa acctaggctg
      241 ctcgagcctt cgctcgaatt ccgattgccc ggctagcacc acttgcacca gcttgtcacc
      301 tgcgtgctcg agatcggaaa ccagcctcaa ctcttcgagg acttcatctt ggatatcctg
      361 ggactcgtcg agcagaagtg ccacccggtg atcgtgatta cgttgctcga gtaagtaatc
      421 tctgaactgc tgcatcagag aaacacggtc tgcgctcaac tgcgtcacgc caaaagcatc
      481 gagagcgcat tgcaacaatt caatgaacgt caaatgcgga tcgagaatgc acgctgcgcg
      541 aacgggttgc gccgctcttt ccatgaacag tttaatgaga gtggttttgc ccgttccggc
      601 tgcgccggtg atgagtacta tacccttgcg gttttcgacg ccataacaga gggtcgcaaa
      661 cgcttcctgg cacgacgcat gagtatacag gaatctcgga tctggagtga gattgaaagg
      721 cagctcgcgt aacctgaaat gcttttcgta catggcgtcg agccccctgg gcaaagcgct
      781 ttacccagtt tgtcatcccg tgcggcagcg gaactacgct ggacctgatt tcgccgcagg
      841 gactgtgcgt tcgcgcggag gcgcagctgg tttttctccg aggggttttt ctttgaaaat
      901 ttttttgtcc aacccgtagg tccgcatctt tctggagagt gttctcaccc cgatcctgca
      961 gcttttcgcg gtcaggctga tgttaccgcc aagatcttcg agctttcgcg acaagtaata
     1021 tttttccttt tctctaagcc actgccgaag cgacgatgcc gcaatgttgc ttttctctgc
     1081 ggcaccgtcc tcgaagcttt ccggcagctt tacgtcttcg atgacccgtc ccggcgcgag
     1141 cagaatcgca cactccagag cgttgtgcaa ttctctcaca ttacccggcc atgaatagcg
     1201 catgagctgg cctaaaactt tgtcggccac agctgcgatc cgcttgcttt tcgcaaccgg
     1261 atgataacgc agaaaatgct ccaccaggtg aggaatatcg atggcgcgtt cgcgcagagg
     1321 cgcgaggtcg atcggtacag cgctcagtcg tgctagcaac tcgtcgcgca gcacccggcc
     1381 cggggctctc tcctttagag cgagtttagt tgccgctata atacgtacat cgatgtgaga
     1441 ggcctggctt gcgcccaatc gggaaatttt cctgtggtcg atgacctcga gcaacttttt
     1501 ctgcagcgcc aacgagagga cttctatttc gtccagaaac aacgtgccgc catgagcctg
     1561 agcgatcttg cctggtttac tgtcataaat accagcaaac gcgtctttct catagccaaa
     1621 taattcagct tgaagcagcg catcgggata tccggcacag ttaaccgcaa taaacggagc
     1681 gctccggcga gcgctatggt ggtgaatggc aaaagcgact tgctccttga ccgttccggc
     1741 ttctcccgcg atcatcaccg gcatgtcaat gggcgcagcc gtgcagatca gctcaaaaac
     1801 tcgacgcatt tgcggcgctg ggctcaaaat atccccgaat tcgaagtgac ctttcagcca
     1861 gcgtctaagc tgccgtgcct gaatgcccgg ctttgaattt tccaaagccg cccgtgtcga
     1921 ttcgagcact gcggctggat caaagggctt tacgacaaaa tcacaggcgc cgagtttaag
     1981 cacgtcgact gtactttgaa tatctgctgt ggaagtaatc gcaacgacgg ggagcgctgg
     2041 gtatctttga tgaatctgtg cgatcagttc gagggcatca gcgtccggaa ggtgaatgct
     2101 cgcaatgata aagtcgatgt cgtcttctgt tagccgccgc atgcaatcgg cggcggaggc
     2161 tagcccgacg acggaatatc cgctggtgag ataaagccgc tctagttgct cacgtatttg
     2221 gtgatcgcca tcgagaacga gcaaagtcgg agccgccgga caaaacaaat catcccgcga
     2281 cgcaggatct cttacgccgg atatctcgag agtggtcgag cccgaatatg tcgctggaat
     2341 tctcatggag ctgccgacgc tttttcgcag atgacctcaa cagaagagga gacgaagaat
     2401 tagggacgag ctcgtcgtca gcagccgcac gcgtacaggt aaagcgatct caaattgtcc
     2461 ctaaaggtga ggtcgtggtt cctcccgagg atggagttct tccacatgta gcccaagtcg
     2521 ctgtgcgtcg acgaagcagt aataacaaac caccatgtcg agcaatgtaa taccggactc
     2581 ctcactcgtg accctgtacg cctgtcccgc catgatttgg ccgcaatgct ggcattggta
     2641 agtcataaaa ctctagtgtg agcaaaggga gtgccactgc ggtcgaatgc tcctgccgta
     2701 ctcaaaaaag tcccctattt ccgtgacacg atagaatgtt cctgtggtat gtttgagctt
     2761 gtatccgaaa aagctgacgg atttaaccga catgagtatc gtaaaatgtg cgtcagttgc
     2821 ggacagattc gctggtgctt ccaccttcct cagcagcggc aggagcgtga gaaactacag
     2881 tttataaacg ttgggctgcg ccaaatcgct gagcgcattg cgggtatcga agatcagcgg
     2941 actgaattta gtgatcatcg cgtaatcgaa tcccgaatgg tcggtgagaa ttacgaccaa
     3001 atccatcgac tgcaacaact cgggactgag cgtgacagag tctagattcg tttcattgac
     3061 cgtaatctgc ggcacatacg gatccgagaa acagatttca cagcctttct cttgaagacc
     3121 caacaacact tgtaatgccg gagattctcg cacatcgttg gtgtcccgct tataggccac
     3181 cccaagagcc aggaccttgg cgccgttcaa acatttgcgt ttttcgttaa ggacatcggc
     3241 cataccacgg atgacgaaag ccggcatctg gctattgatg tgtcctgcaa gctc
//
LOCUS       DASULZ010000302         3885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_39174, whole genome shotgun sequence.
ACCESSION   DASULZ010000302 DASULZ010000000
VERSION     DASULZ010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39174"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VFY96_06320"
     CDS             complement(<1..749)
                     /locus_tag="VFY96_06320"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX5606111.1"
                     /translation="MVLLHVFRLLIVVSALGFGSSVRAQERPFPVPDGLEGAVSFWKQ
                     IFAQYSTNEVVLFDPVDPGTIYTVVRAGDNEEGRALLEKERARVIAEYDLADDESRIR
                     SQRGAKEHFTEGLKIAGRYISAMKKIFRGEGLPVELVYLPLVESSFNVRARSGAGAVG
                     MWQFMPDTGKKFLRIDDNIDERRDPFESTKAAARLLKENYRLLGSWPLAITAYNHGTD
                     GMFRGIKAVESDDIVDLIRQYRSPTFGFASK"
     gene            831..2381
                     /locus_tag="VFY96_06325"
     CDS             831..2381
                     /locus_tag="VFY96_06325"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606112.1"
                     /translation="MRRSLLHFFSVALAFTFLAACRPTHSDHSSGYLIAAIESYPLQL
                     DPRYATDANGVRIGNLIYNSLLRADSQSRLRPELAESLRLFDDRSYVVDLRRDVKFQN
                     GQPLTAADVKFTYESILDPNSRSPKRGNLKPLRAIEQTGAYQLRFHLFAPHSPFIEQL
                     TLGIVPAGSPVNSAGPTQAPPGSGPFILEEAEPGEQIRLRRNPLYWEQKPSLAGLSFR
                     IIPDALVRVLEFKKGNIDLMQNDIEPDMVPWLERNTGADVAAYPGTTFQYIGINLTHP
                     ILKHVTVRRALAYAIDRDRLVRHILKGIGTVAGGVLSPLNWAYEENVEHWPYDPERAK
                     QLLDEAGFPDPDGDGPLPRFRLSFKTTNIDLRRRIAEAIKEQLQKVGIELDVRSYEWA
                     AFYSDIKKGSFHLYSLAWVGILDPDILFQIFHSSSFPPAGDNRGHYSNPELDRLLEQG
                     RAISGQQERRQIYARAQKILAQDLPYVPLWWWKNVIVKKPIVQNFTPYPDGEMISLKQ
                     TRWAAKTQ"
     gene            2378..3301
                     /gene="nikB"
                     /locus_tag="VFY96_06330"
     CDS             2378..3301
                     /gene="nikB"
                     /locus_tag="VFY96_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease"
                     /protein_id="HEX5606113.1"
                     /translation="MKRYVLYRLALLLPTVFGVLTLVFLFIHLAPGDPVDIMLGETAS
                     AADRQELRQILGLNQPLTEQYRSFLINFFRGDLGQSLYEGRSVAAMVSARYPATLELA
                     LCAMSTALLISFPLAIWSAVRRGRWSDRATLMFSLLGLSMPNFWLGPLVMIIFSIQLG
                     WLPVSGRGGPDHLVLPALTLGLGMAALLIRILRTNLLRVMREDYVQTARAKGLRERSV
                     WLKHTLRNALLSLVTIVSLQFGSLLAGSLITETIFSWPGIGRLTIQAIEMRDYPLVQG
                     CVLAIALSYVFLNFITDLIYHWVDPRITYGK"
     gene            3291..>3885
                     /locus_tag="VFY96_06335"
     CDS             3291..>3885
                     /locus_tag="VFY96_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5606114.1"
                     /translation="MASRFWLYAGAALLLLFASAALFAPVVSPYDPLQQNLEQDLITH
                     SPQHPLGTDKLGRDLLSRIIFGARISLLVGISTVALSLLIGVGVGSLSGYAGGIADLL
                     FTRFIDILMAFPGILLAIAFSAVLGPGLDHVIVALALIGWTGYARLVRGEILSLRERD
                     FISAARALGCRPQRIIVLHLLPNLLPPLAIQATFGLAA"
BASE COUNT          796 a         1136 c          988 g          965 t
ORIGIN      
        1 tttttcgacg cgaagccaaa cgtcggcgac cgatactggc ggatcaggtc gacgatatca
       61 tccgattcga ccgctttgat gccacgaaac attccgtcgg tcccgtggtt ataagcggtg
      121 atggccagag gccaacttcc cagcagccgg tagttttctt taagcaagcg tgccgccgct
      181 ttggtggact cgaagggatc cctccgctcg tcgatattat cgtcgatccg caaaaatttc
      241 ttgcccgtgt ccggcatgaa ctgccacatg cccactgctc ctgcgcccga gcgagcccgt
      301 acattgaacg aagattccac gagcggcaga tacaccagtt ccaccggcaa accttcgccg
      361 cgaaatattt ttttcatcgc agagatgtaa cgaccggcga ttttaagccc ctcagtaaag
      421 tgttccttgg caccgcgctg gctccgaatc cggctttcgt catcggcgag atcgtattcg
      481 gcgatcacgc gagcgcgctc tttttcgagc aatgcgcgcc cttcctcgtt atcgccggcc
      541 cgcaccacag tgtagatcgt gccgggatcg acgggatcaa acagcacaac ttcattggtg
      601 ctgtactgtg caaaaatctg tttccaaaaa ctcaccgcgc cttctaggcc gtccggcacg
      661 ggaaacggcc tttcctgagc tcgaaccgag ctaccaaagc caagagcgct caccacaatc
      721 aagagccgaa acacatgcaa gagaaccatg aatccacccc aagccaaaaa aaatttgcct
      781 gatcttagca aacaggctat tttcagtcaa aaattttcgc cgagaatata ttgcggcgtt
      841 cgcttcttca ctttttttct gtcgccctcg cctttacttt tttagcggcc tgccggccaa
      901 cacactcgga tcattcatca ggttatctga tcgccgccat tgagagctat ccgcttcagc
      961 tcgacccacg ttatgccacc gatgccaatg gcgtccgcat aggcaatttg atttacaatt
     1021 ctttgctccg tgctgattca cagtcgcgct tacgacctga gctcgcggaa agcttacgcc
     1081 tcttcgacga tcggagttac gtggtcgact tacgccgtga tgtcaaattt caaaatggcc
     1141 agccgctcac tgccgccgac gtaaaattca cctatgaatc gatactggat cccaacagcc
     1201 gctcgccgaa acgtggcaat ctgaagcccc ttcgcgccat cgaacagaca ggggcttacc
     1261 agctccgttt ccatctcttc gctccccatt cacctttcat cgaacagctc acgctcggaa
     1321 tcgtacccgc gggttcgccg gtaaattccg ccgggccgac gcaagctccg cccgggtccg
     1381 gtccgtttat cttggaagaa gcagaacctg gcgagcagat caggctcaga agaaatccgc
     1441 tctactggga acaaaagcca tctcttgccg ggctcagctt tagaattata cccgatgctc
     1501 ttgtgcgggt actggaattc aaaaagggca acatcgatct gatgcagaat gacatcgagc
     1561 ctgacatggt gccctggctc gaaagaaaca cgggtgcaga tgtcgccgcg tatcccggca
     1621 caaccttcca atacattggc atcaacctga cccacccgat tttaaaacac gtgacagtcc
     1681 gccgggcgtt ggcgtacgcc atcgatcgtg atcgtttagt gcgacatatt ttgaagggca
     1741 tcggaacggt tgccggcggt gtgctctcac cgctcaattg ggcgtacgag gaaaatgtcg
     1801 agcattggcc ttacgatccc gagcgcgcta aacaactcct cgatgaagcc ggttttcctg
     1861 atcccgatgg cgatggaccc ttgccgcgtt ttcgcttgtc cttcaaaacc accaatatcg
     1921 atttgcgccg acgtatcgcc gaagcgatta aagaacagtt gcagaaagtt ggcatcgagt
     1981 tggatgtccg cagctatgag tgggcagcat tttatagcga tatcaaaaag ggcagttttc
     2041 acctttactc gcttgcctgg gttggcatcc tggatccgga tattctgttt cagattttcc
     2101 attcctcgag ctttccgccg gcgggcgaca atcgcggcca ctacagcaac ccagaactcg
     2161 atcgcttgct cgagcagggc cgagcgatta gcggccaaca ggaacgacga cagatttatg
     2221 cgcgggcgca aaaaattctc gctcaagatc ttccctacgt gccactctgg tggtggaaga
     2281 atgtgatcgt caaaaaaccg atcgtgcaaa attttacacc ctatcccgat ggcgaaatga
     2341 tctcattgaa acagacccgc tgggccgcga agactcaatg aagcgatatg ttctttaccg
     2401 cttagctctc ttgctgccga ccgtattcgg agtgctgact ttggtatttt tgttcattca
     2461 tctggctccc ggtgatcccg tcgatatcat gctaggcgaa actgccagcg cagccgaccg
     2521 ccaagagcta cgccagattc tcggcctcaa ccaaccgcta actgagcaat atcgctcttt
     2581 cctaataaat tttttccgcg gcgatctcgg ccaatcgctg tatgagggtc gcagcgttgc
     2641 tgctatggtg agcgctcgct atcccgcaac gctcgagctc gcgctctgcg cgatgagcac
     2701 cgcgctcctg atttcattcc cgctggctat ctggtctgca gtccggcgcg gtcgttggag
     2761 cgatcgcgcc actctgatgt tttctctgct tggactttcg atgcccaatt tttggctcgg
     2821 ccccttggtc atgatcatat tctccatcca actcggctgg cttcccgtat ccgggcgcgg
     2881 cggtccggat catttggttt taccagctct taccctcgga ctcggtatgg cggcgcttct
     2941 gattcgtatc ttacggacga atctgttacg cgtcatgcgc gaagattacg tgcaaacggc
     3001 tcgcgccaag ggtttgaggg agcgctccgt ctggctgaaa cataccttgc gtaatgcgct
     3061 gctctcgctg gtcacgatcg tcagccttca gttcggtagt cttttggcgg gatcattgat
     3121 taccgagacg atttttagct ggccggggat tggacgtctg acgatacaag ctattgagat
     3181 gcgcgattat ccgctggtgc aaggctgcgt attggcgatc gcgctttcct atgtcttcct
     3241 gaatttcatt acggatctaa tttaccactg ggtggatccg cgcatcacct atggcaagta
     3301 ggttttggct ttatgcggga gcggcgctgc tgttgctgtt tgcgagcgcc gccctgtttg
     3361 cgccggtcgt gagtccctat gatccactcc agcaaaatct cgaacaggat ctgatcaccc
     3421 actcgccgca acatcccctg ggcacggaca agctcggacg ggacctgctg agtcggatta
     3481 tttttggcgc ccgtatttct cttctcgtcg gaatctcgac cgtagcgctt tcgcttctga
     3541 tcggcgtcgg cgtgggttcc ctttccggat atgccggcgg tattgccgat ctgctgttta
     3601 cgaggttcat cgatattttg atggcttttc cgggtatctt actcgccatc gctttcagcg
     3661 cagttctggg cccggggctc gatcacgtga ttgtcgcgct ggctttgatc ggttggaccg
     3721 gctacgcgcg gctcgttcgc ggtgaaattc tttcgctgcg cgaaagagac tttattagcg
     3781 ccgcgcgcgc actcggatgt cggccgcagc ggatcatcgt gctccatctg ttaccgaacc
     3841 tgttgccgcc gcttgcgatc caagccacct ttggcctggc cgcgg
//
LOCUS       DASULZ010000303         3290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1358234, whole genome shotgun sequence.
ACCESSION   DASULZ010000303 DASULZ010000000
VERSION     DASULZ010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358234"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VFY96_06340"
     CDS             <1..826
                     /locus_tag="VFY96_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX5606115.1"
                     /translation="VFNNKILNTNSQGFRGKNDFSYSKNKQKLRILILGDSFTFGDEV
                     SDDETYSFYLQQMLPQAEVINMGVHGYGHDQMLILLWEEGIKYEPDIVIVGFLGLDMS
                     RNLLSFRDFAKPRYVLEKGRLRLTGTPVPRPEEILKWDWARPRVIDIFSLTSHAVQKF
                     SGIQKQEMQDITRAIVREMINVVESVHATLILEYLPRGKEIALRTAETEDEAYMLSIC
                     RPDDKAKCFSTRPHFAQKIAEGMRFKAGGHWEPAGHMVVAEAIKHHLVSEGYIVPQ"
     gene            complement(967..1041)
                     /locus_tag="VFY96_06345"
     tRNA            complement(967..1041)
                     /locus_tag="VFY96_06345"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1006..1008),aa:Arg,seq:ccg)
     gene            complement(1074..1883)
                     /locus_tag="VFY96_06350"
     CDS             complement(1074..1883)
                     /locus_tag="VFY96_06350"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX5606116.1"
                     /translation="MLVDTDKLLEVGWEGASAAGQIIQESWRKPRSIEYKGALDLVTS
                     VDRESERIIVATIQHYFPDHSILAEEETLVHGADQECRWIVDPLDGTTNFAHGYPQFC
                     ISIAFENRGIVTMGLVYDPVRQECFKAVRSGGASLNGATIHASPLAQLDKALLGTGFP
                     YDRRDFADLYLSYFKAFMVRSQGVRRGGSAALDLCYVASGRFDGFWEMKLKPWDVAAG
                     ALIITEAGGVLSDFYGNAFSIYGQETLASNRLIHREMVEIASAIAKETLVD"
     gene            1928..2794
                     /gene="ispE"
                     /locus_tag="VFY96_06355"
     CDS             1928..2794
                     /gene="ispE"
                     /locus_tag="VFY96_06355"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX5606117.1"
                     /translation="MKIRAPAKVNLFLRVLGRRRDGYHLLDTLIVPVSLYDDIEIRRL
                     PAKPQESANPRITIACDNPAVPPGEENLAYRAAQLLLEQVDRRTSVHIYIQKRIPLGA
                     GLGGGSTDAAATLLGFNRLFKLNLSAARLERLALRLGADVPFFIRARPARAHGIGEKL
                     RPVRGLVHFWLVILYPGFPVATGSVYKKLPKKLTKSTPGNSIATFLKRFDRLPDLLVN
                     DLESVTLESYPKIQSLKEKLLREGAAGGLMSGSGSSVFGVFGSKGLAQRAFDRLRNEE
                     GVQAYLVHVLNR"
     gene            2840..3127
                     /gene="spoVG"
                     /locus_tag="VFY96_06360"
     CDS             2840..3127
                     /gene="spoVG"
                     /locus_tag="VFY96_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HEX5606118.1"
                     /translation="MQVTEVRVFPVDEDKLKAYVTITLDHCFVIRDLKIIHGSTGFFV
                     SMPSKKRKDGTYKDIAHPINNETRRMIEEKIIAEYHKVLAEGGSPKALDDM"
     gene            3175..3248
                     /locus_tag="VFY96_06365"
     tRNA            3175..3248
                     /locus_tag="VFY96_06365"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3207..3209,aa:Gln,seq:ttg)
BASE COUNT          807 a          821 c          882 g          780 t
ORIGIN      
        1 ggtgttcaac aacaaaattc tgaacaccaa ttctcagggt ttcagaggga aaaacgattt
       61 ttcttatagc aagaataaac agaaattgcg cattttgatt ctaggtgact cgttcacgtt
      121 cggcgatgaa gtgagtgatg acgagacata ttctttctat cttcagcaaa tgttgccgca
      181 ggccgaagtc atcaacatgg gtgtacacgg ttatggacat gatcaaatgt taattctcct
      241 ctgggaagag ggaatcaaat acgagccgga tattgtcata gtgggttttc ttgggctcga
      301 tatgtcgaga aacttgctgt cgtttagaga cttcgcaaag ccgcgttacg tcctggaaaa
      361 aggcagactg agactcaccg ggaccccggt tccacgcccc gaggagatcc tgaagtggga
      421 ttgggccaga cccagagtca ttgatatttt ttcactgaca agccatgccg ttcaaaaatt
      481 ctccgggatt caaaagcagg agatgcagga tattacgaga gcgatcgtaa gagagatgat
      541 caacgtggtc gagagtgttc acgccaccct gatactggaa tatcttccac gaggaaaaga
      601 aatcgcacta aggacggccg aaacggaaga tgaggcctac atgctttcca tttgccggcc
      661 cgatgacaaa gccaaatgtt tttccacaag accgcacttt gcccagaaaa tagcagaagg
      721 gatgcgcttc aaagccggtg ggcattggga gcctgcgggt cacatggtgg tggccgaggc
      781 aatcaaacat catctcgtca gtgaaggtta cattgtcccc cagtaagaat ttctcgctaa
      841 cctccattgg cttctcgaaa tcacagcccg gttaagccta aggcacagtc aaataatcgc
      901 ccagaaaccg caaccatcat agaaaagcga aattgatgcc tgaggctgag aagtaacgga
      961 aacacatggc gcgcccgaca ggaatcgaac ctgtacttag ggttccggag accctcgtga
     1021 tatccttttc actacgggcg cacgaaaagt tttttgtatc atcggggtgc tactcagtca
     1081 acgagcgttt cttttgcgat cgcacttgcg atttccacca tttcgcggtg aatcaggcga
     1141 ttggaagcaa gcgtttcctg gccatagatg ctgaaggcgt ttccgtaaaa atcactcaaa
     1201 actcctcccg cttcagtaat aatcaatgcg cccgccgcga cgtcccaggg cttgagtttc
     1261 atttcccaaa agccgtcgaa tctgccggac gcgacatagc ataggtccag agcagcggac
     1321 ccgccacggc gaactccctg agatcgcacc atgaacgctt tgaaatagct taggtagaga
     1381 tcggcaaagt cgcgccgatc gtagggaaaa ccggttccga ggagcgcctt atcgagttga
     1441 gcgagtggcg aagcgtgaat tgtcgcgccg ttcagacttg cacctccgct acgcacggct
     1501 ttgaagcatt cctggcgcac tggatcgtaa accaggccca tcgtcactat tccgcgattt
     1561 tcgaatgcga tggagataca gaattgggga taaccgtgcg caaaattcgt cgtgccgtcc
     1621 agcggatcaa cgatccagcg gcattcttga tcggcaccat ggacgagcgt ttcctcttcg
     1681 gcaagaatcg agtgatcggg aaaatagtgt tggatggtgg cgacgataat ccgctcgctc
     1741 tcgcgatcca ccgaagtgac caaatcgagg gcgcctttgt attcgatcga cctcggtttt
     1801 cgccaactct cctgaataat ttgacccgcc gcgctcgctc cttcccaccc aacctcgagt
     1861 aacttgtcgg tgtctacgag catcgctagt ttcgcaaatt gtttgcgccc atgttagcta
     1921 atggcacgtg aagattcgag cgcccgcgaa agttaatttg tttttgcgag ttctcggtag
     1981 gcgtcgggat ggctatcacc tgctcgacac gttgattgtt ccggtgtcgc tctacgatga
     2041 tatcgaaatc cgccgactgc cggcaaaacc tcaggaatcg gcaaatccgc gcatcacgat
     2101 agcttgtgac aaccccgccg tgccgccggg cgaggaaaat cttgcctatc gcgctgcgca
     2161 attgttattg gaacaagtcg atcgccgcac aagcgttcat atttatatac agaagcgcat
     2221 tccgctcggc gccgggttag gtggagggag tacggatgcg gcggcgactc tgctcggttt
     2281 caatcgattg ttcaagctta atctatcggc agctcggctg gagagattag cgttgcgact
     2341 tggggccgac gtgccgttct ttattcgcgc gcgtcctgcg cgtgctcacg gcattggtga
     2401 aaaactgcgc ccggtccgcg ggctggtgca tttttggctc gtaattcttt atcctggatt
     2461 tcccgtcgcc actggttcgg tgtacaagaa actgcccaaa aagttgacaa agtcgacccc
     2521 aggtaatagc attgcgactt tcttgaagcg gtttgaccgg ctacccgatc tgctcgtgaa
     2581 cgatctggaa agcgtcacgc tcgagagcta tccaaaaatt cagtcattaa aggaaaagct
     2641 attgcgcgag ggggccgcag ggggtttaat gtccggtagc ggctcttctg tattcggcgt
     2701 gtttggctcg aagggactgg cacaaagagc gtttgaccgt ttgcggaatg aggaaggggt
     2761 gcaagcatat ttggtccatg tgctgaatcg ttgatccgcc ggaaagccta tagtaaatcg
     2821 caacgagttg ggggtcccta tgcaggttac cgaggttagg gtctttcctg tggatgagga
     2881 taaactaaag gcttatgtca cgatcaccct cgaccattgt ttcgtgatcc gggacctgaa
     2941 gatcatccat ggctctactg ggttctttgt ctcgatgccc agtaaaaagc gcaaggacgg
     3001 aacctacaaa gacatcgctc atccgatcaa caatgagact cggcggatga tcgaagaaaa
     3061 aatcatcgcg gaatatcata aggttctcgc cgaggggggc agtccaaaag cgcttgatga
     3121 tatgtgagcc gatatccgcg accgagtgaa ctcctgggat aggagttcgt gcgatgggcg
     3181 gtcgccaagc ggtaaggccc cggactttgg atccggtatc gaaggttcga atccttcccg
     3241 cccagccact ttagcgattc gttggcttaa cagggtatta tttggtatct
//
LOCUS       DASULZ010000304         2828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_748417, whole genome shotgun sequence.
ACCESSION   DASULZ010000304 DASULZ010000000
VERSION     DASULZ010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748417"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1910)
                     /locus_tag="VFY96_06370"
     CDS             complement(<1..1910)
                     /locus_tag="VFY96_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HEX5606119.1"
                     /translation="MSDELAASMGRFRELTGASQKLLRALLITSTVLGSSWALGVHNY
                     FQITFFKQQYLALFLALALGAVFLAVKAYPKQSGEFVPWYDWLLALGGFAIGIYVTVM
                     YPIIAYRLGVLSPDRWILGGLALILVLEATRRVAGGTLAWLALAVILYTKFAEFLPGI
                     LYSKSPSWARIVSYLYLDSNAMLGLPIDVAAGVVVAFILFGQALYAVGGDKFLTDLAL
                     IATGRYRGGPAKVSVVSSALFGTVSGSAVANVVVDGAITIPLMKRTGFAAHMAAAIEA
                     VASNGGQIMPPVMGAAAFLMAEFLNLPYGQIALAAAIPALLYYLALFTQIDLEAAKHG
                     LAGLPADQIPRFRNIMKSGWVFLIPLGLLIYTLMIENWEAGKAGIVAVLTVFGVGALQ
                     RSTRPSFAKVTAALEETGKTLLDIAVITALAGIVIGALHLSGFTFKISLLLVTLSGNN
                     VFLLLLITAFGCLFLGLPLPTTVVYITLAVLAAPALVQLGIPPLAAHLFLFYFGMVSL
                     ITPPDCLPVYIAASMARANFWQTGWTAMRLGIAAYIVPFIFALHPPLILIGNLRDILI
                     AVVTAVIGIVLLGAGCAGYLFRPLGWNKRGLLWIAALLLLLPSTTGVLLLGDLIGFIL
                     GAAVIGWEWTQR"
     gene            complement(2001..>2828)
                     /locus_tag="VFY96_06375"
     CDS             complement(2001..>2828)
                     /locus_tag="VFY96_06375"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX5606120.1"
                     /translation="GTSTFVPLFDSGELELGVVNAVDMGMVYQGQKLKVGGRNPFPHV
                     ASSRLIMRGSPLRSSLIVRKDSPIKTIAEVKGKRVTGEYPAQLAVWYNVFGSLSNGGL
                     TWNDVKVVPVPAVNEGVDALVQGRADVTTHAIGSAKVKEADAAVGIRYIPLDCSKQGE
                     ARIKKAVPGYYLSIVKAGSSTGIVEDTCAYTYDIYLVGPKTLPDPVVRNVLQAIWINV
                     EKLPQFHPSFAEWTKARAVDPEVTLPYHPAAIRFFKDNNIWPAKMDDAQKRLLALNP"
BASE COUNT          705 a          768 c          844 g          511 t
ORIGIN      
        1 tcccgctgcg tccattccca cccaataacg gctgcaccta gaatgaagcc gatcaggtcc
       61 ccgagaagca gcacaccagt cgtggagggt agaagcagca gcagcgcggc gatccagagc
      121 agcccgcgct tgttccagcc cagcggccgg aacaagtagc cggcgcaacc ggcgccgagg
      181 aggacgatgc cgatcaccgc ggtcacaaca gcgatgagaa tgtctctgag gttaccgata
      241 agaatcagcg gtggatgaag cgcaaaaata aatggcacga tgtaggccgc gatccctaaa
      301 cgcatcgcgg tccagccggt ctgccagaaa ttcgctcgcg ccatcgaggc ggcaatgtaa
      361 accggcagac aatccggcgg cgtgatgagc gagaccatcc cgaaataaaa aagaaataaa
      421 tgcgccgcca gcggcggaat accgagttgc actagagcgg gcgcggcgag gacggcaagg
      481 gtgatgtaga ccactgtggt cggaagcggc aaacccaaaa ataagcagcc aaaggcggtg
      541 atcagcagaa gcagaaagac gttgttaccg gagagagtca caagcaagag tgagatcttg
      601 aacgtgaagc cggaaagatg aagcgcgcca atgacgatcc ctgccaatgc ggtaatcacg
      661 gcaatatcga gcagtgtttt gcctgtttcc tccaatgcgg cagtgacttt ggcgaaggac
      721 gggcgagtcg atctctgcaa tgcgccgacg ccgaacacag tgaggacagc aacgatgccg
      781 gctttaccgg cttcccagtt ttcgatcatc aaagtataga tgagaagtcc caacggtatc
      841 agaaagaccc atcctgattt catgatattt cgaaatcgag gaatctggtc tgcaggcaat
      901 ccggccaagc cgtgtttcgc cgcttccaga tcgatctgcg taaacagcgc gaggtagtag
      961 agcagcgcgg gaatcgctgc cgccaaagcg atctggccgt acggcaaatt gagaaattcg
     1021 gccatgagga aggcggcggc gcccatgacg ggcggcatga tctggccgcc gttcgatgcc
     1081 acggcctcga ttgcggcggc catgtgggcg gcgaagccgg tgcgcttcat gagcgggatg
     1141 gtgatcgcac catctacgac gacgtttgcc acagcgctgc cggaaactgt gccgaagagc
     1201 gccgaagaaa ccacggatac ttttgccggt ccgccccgat aacggcccgt ggcaatcagc
     1261 gcaagatcgg taaggaattt atcgccaccg accgcataga gcgcttgccc gaacaaaata
     1321 aaagccacca ccacaccggc ggccacgtca atgggtaaac ccagcatggc gttcgagtcg
     1381 agataaagat aactcacgat gcgggcccac gacgggcttt tggaataaag aattcccggc
     1441 aggaactcgg caaatttggt gtagagaatg acggcgagcg caagccaggc caacgtgccg
     1501 ccggcgacac ggcgcgtggc ttcgagcacg agaatcagcg cgaggccgcc gagaatccag
     1561 cgatccggcg agagcacgcc gagccggtac gctatgatcg gatacataac ggtgacgtaa
     1621 attccgatcg caaaaccgcc aagcgcgagg agccaatcgt accagggaac gaactcgccc
     1681 gactgcttcg ggtaggcttt gacggcgaga aagacggcgc cgagcgccaa ggccaaaaat
     1741 aaagcgagat actgctgttt gaaaaacgtg atttgaaagt aattatgaac tccgagcgcc
     1801 cacgatgaac ctagaacagt gctggtgata agcagggcgc gaagcaactt ttgtgacgca
     1861 ccggtcagct cacgaaaacg gcccattgac gcagctagtt cgtcgctcat cgggtagaac
     1921 gggtcagctc aaaacgttcg agcggttcgc atggttggat tgttggcggc caaattgccg
     1981 gacgaagtcc tatttcgatg ttacggattc aaagcgagca gacgtttttg cgcgtcgtcc
     2041 attttggccg gccagatatt attatccttg aagaagcgaa ttgcggcggg atgataaggc
     2101 agagtgactt cgggatcgac agcccgcgct ttggtccact ccgcaaaact aggatggaat
     2161 tgcggcagtt tttcgacgtt aatccagatg gcctgcagga cattgcgaac gacgggatca
     2221 ggcaatgtct tgggaccgac gagataaata tcataggtgt aggcgcaggt atcttcgacg
     2281 atcccggtgg acgaaccggc tttcacgatc gaaaggtaat aacccggcac ggcttttttg
     2341 atgcgtgctt cgccctgctt agaacagtcg agcgggatat agcgaatacc gacggcggcg
     2401 tcggcttctt tcaccttcgc cgatccgatc gcatgcgtag tgacgtcggc gcgtccttgc
     2461 accagggcat cgacgccttc gttgacggcg ggcactggaa cgactttgac gtcgttccag
     2521 gtcaggccgc cgttcgaaag cgagccgaag acgttgtacc aaaccgccag ttgcgccgga
     2581 tattcaccgg tgacccgctt gcccttgacc tcggcaatgg ttttgattgg cgaatccttg
     2641 cgtacaatca ggctgctgcg caatggcgag ccgcgcataa taaggcgcga gcttgctacg
     2701 tggggaaagg gatttctgcc gccgacttta agtttctgcc cttggtatac cattcccata
     2761 tcgacagcgt tgacgacgcc gagctcgagc tcgccgctgt cgaacaacgg cacgaacgtg
     2821 ctcgtgcc
//
LOCUS       DASULZ010000305         2477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1358371, whole genome shotgun sequence.
ACCESSION   DASULZ010000305 DASULZ010000000
VERSION     DASULZ010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358371"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="VFY96_06380"
     CDS             <1..833
                     /locus_tag="VFY96_06380"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEX5606121.1"
                     /translation="QTARGQEVICEAAGHIYNYEMASMSAIGAVLPRVIPTEYGIMRW
                     DQIDAAIREKSYARAQTALVALENTHNMAGGTVYPTPLAHEICEKAHGVGIKVHLDGA
                     RVFNAAAYLGQSVAEMTRCFDSVQFCFSKGLGAPVGSMIVGSRDLIERCRVIRKMLGG
                     GMRQVGVLAAAAEIALEEGPKRLHIDHENAHLLAAGLASCPRIRIEPEKVQTNIVLFD
                     VSETGWTSGQFLRRLSEQKVLGGPVDAHRVRMVTHLDVDRADIEQALGVIGKLVGAGF
                     "
     gene            complement(841..1188)
                     /locus_tag="VFY96_06385"
                     /pseudo
     CDS             complement(841..1188)
                     /locus_tag="VFY96_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
     gene            complement(1201..1335)
                     /locus_tag="VFY96_06390"
     CDS             complement(1201..1335)
                     /locus_tag="VFY96_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606122.1"
                     /translation="MARQLGPGDKFPEYRVSAVGNRTLQLPTDLHGAYAVILFYRGIW
                     "
     gene            complement(1369..2286)
                     /locus_tag="VFY96_06395"
     CDS             complement(1369..2286)
                     /locus_tag="VFY96_06395"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5606123.1"
                     /translation="MIIDCHAHLVPKSWYHPKSPRSIFDIDELFREQEQSGVNLTVFG
                     NNWIRSPDGYDPLKVVQEFNAHAAELTAKYPKRLLGLACSVPYDNDEILKETEKAIRD
                     YKLKGIMIGSSTNGEYLDSPRATPFFQLVNELDVPLFVHPPKFTIGNEKMEIFRLPEM
                     LGRPFDTTLSLTRFILTGGFEKFPNLKMVCAHVGGALPMLPGRLGFGYELRKDDSFGP
                     WEPDVMTRPPASYMKQLYFDTVCYHPPAVQCAIDTVGVEHVVFGSDSPPVPLPLARAV
                     DTVKQLKISEEDKQKIFGDNAAKLLKLAG"
BASE COUNT          555 a          659 c          676 g          587 t
ORIGIN      
        1 cccaaactgc ccgtgggcag gaagtcattt gcgaggctgc cggtcatatc tacaattacg
       61 agatggcctc gatgtcggcc atcggcgctg ttttgccgcg tgtaattcct accgagtatg
      121 gaattatgag gtgggatcaa atcgacgccg caatccgcga aaaatcctat gcccgcgcgc
      181 aaaccgcgtt ggtagccttg gaaaatactc acaacatggc gggaggcact gtttatccga
      241 ctccgctggc gcacgaaatc tgcgagaaag cacacggcgt cgggatcaaa gttcatctcg
      301 acggcgcgcg tgttttcaac gctgctgctt atctcgggca aagtgtcgcc gaaatgacgc
      361 gttgcttcga ttccgtgcaa ttctgcttca gtaaagggct tggcgcgccg gtgggttcga
      421 tgattgtcgg cagccgagat ttgatcgagc gttgtcgagt cattcgcaaa atgctcggcg
      481 gcggaatgcg ccaagtcggc gtgttggcgg cagccgccga aatcgctttg gaagagggac
      541 cgaagcggct gcatatcgat catgagaacg cgcatctttt ggccgcgggt ttggcatctt
      601 gcccgcgcat tcgaatcgaa ccggagaaag tccagaccaa tatcgtgttg ttcgatgtga
      661 gtgagactgg atggacgtca ggtcagtttt tgcgacgcct ctcggagcag aaggtgctag
      721 gcggtccggt cgacgcgcac cgtgtgcgca tggtgactca cctcgatgtc gatcgggcag
      781 atatcgagca ggcgctaggg gtgatcggga agcttgttgg cgccggattt taactgagat
      841 ttagctagcg ctctgtgcct ttcgacgctt ggctcccctg atcgaactta gtgcatcggc
      901 tgcaacgatt cgtcctatcg gtcccgtcgc aaaacaggct tgtagaattc tatgatccgg
      961 cccgaccgta aagccggtcg cgtgaatgat gccgcgacgc tgttcccaat aggcgccgac
     1021 cttttcggcg tcctccggta cttttaaccc gtagccaatg gaaaatgtgg caccggtgcg
     1081 ctcgaccatt tcttgcgcct tgtctaaagg atcgacagaa agggcaacga cctcgatctt
     1141 ctcttgtttg aactcttcga tatacgcctg gtaatcggcg acctgccgaa cacaatacgg
     1201 tcaccagatg ccgcgataga atagaatgac tgcatatgct ccgtgaagat ccgtcggaag
     1261 ctgtagggtg cgattgccga cggcggatac acggtattcg ggaaatttgt cgcccggccc
     1321 tagctgtcgc gccatgttat cgtcctcctg gaactatacg caccctgttc aaccggctaa
     1381 tttgagaagc tttgccgcat tgtcgccgaa gatcttttgc ttgtcttcct cgctgatctt
     1441 caattgttta actgtatcga ccgctctggc gagcggaagc ggcaccggcg gcgaatcact
     1501 gccaaacacg acgtgctcga caccgaccgt atcgatcgca cactgtactg caggcggatg
     1561 gtagcacacc gtgtcgaaat agagttgctt catatagctt gctggcggcc gggtcatcac
     1621 atcaggctcc cagggtccga aactatcatc ttttctcaac tcgtaaccaa accctaatcg
     1681 ccccggcagc atcggcaggg cgccgccgac gtgagcgcaa accatcttga ggttgggaaa
     1741 tttttcaaag ccgcccgtga gaataaatcg ggtcaaggac agagtcgtat cgaacggtcg
     1801 accgagcatt tccggcaagc gaaaaatttc catcttttca ttgccgatgg taaattttgg
     1861 cgggtgtaca aacagcggca cgtcaagctc attgactagt tgaaaaaacg gtgtggcgcg
     1921 cggcgaatcg agatactcac cattggtgct tgagccgatc ataatcccct tgagcttgta
     1981 gtcgcgaatc gccttttcag tctccttgag aatttcgtca ttatcgtagg gcacagagca
     2041 ggctagcccg agcaagcgct tcggatactt cgccgttaac tcggcggcgt gcgcattaaa
     2101 ctcctgaaca actttcaatg ggtcataacc atccggtgag cgaatccagt tgttgccgaa
     2161 caccgtaaga tttacaccgg attgctcctg ctccctgaac agttcgtcga tatcgaagat
     2221 cgagcgcggc gacttgggat ggtaccagct cttcggaacc agatgtgcgt gacaatcaat
     2281 aatcatcgta aaatcttcct ttcgacaaat cttagcgggg ctgcaatgca gatgtcaatt
     2341 tgcctaggta atctacgatg aagcacagtt ttatgccact cctttacgcg cgttcaggag
     2401 cgcatttaaa ccgtgtcaga cgttgacgcc acgtaacact gaaccatagt cttgtcagaa
     2461 cgatgattcg cgatcgg
//
LOCUS       DASULZ010000306         5717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_952245, whole genome shotgun sequence.
ACCESSION   DASULZ010000306 DASULZ010000000
VERSION     DASULZ010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952245"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1236)
                     /locus_tag="VFY96_06400"
     CDS             complement(79..1236)
                     /locus_tag="VFY96_06400"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5606124.1"
                     /translation="MWNGMKVIDADAHMHEPQYLWERYVEPKFRDHVPKVAFMDGVFM
                     VYEPDGKIIPKGDLQLRPPPSAWAVLEEKYGEAYRQWWSSEIRLKDMDRYGWDIQVLL
                     PTGNNGNFAYRVALKDVELGAAMCRAYNNWCHDYCSADPKRLKFVAVLPGSDIREMVK
                     EARRAVTELGAVSVRNPFLPDGRYLHEPEYDALWQLASELDFPISAHGEYRQRRFHPF
                     RQLEGQRRGDTELALRGLDHALGFPCDNMTTLGHFIFTGILERFPKLRLAILESNAGW
                     LPFWLGRMDDHTHGRNSVMGKSEKLRMLPSEYFMRQCVISCDSDERALKYVVEDLNGE
                     NIVWNTDYPHPDAIDPKRALPDLDAQPIPNEAKRKILWDNAIKLFGPRILN"
     gene            1540..2187
                     /locus_tag="VFY96_06405"
     CDS             1540..2187
                     /locus_tag="VFY96_06405"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5606125.1"
                     /translation="MRANTFLIVLCIATSLWAWQQNPTFAQQNLVFSYNNLLQRRVWT
                     LVMALFVHANLLHLIGNMLFLFIFGNTLEKRAGARAYLAAFFCGGFSSFLLSPLFMPH
                     RAGMLGASAAIFTVAACVMLIQPLKFSWLFLAPQGLVALIYFIYNLVVVSHPSLVRGY
                     DPHVAYIAHIIGFLVGIPFGVALSRQWKRNLLLTLLLFAIYLVILHAARDLFLRR"
     gene            complement(2579..2908)
                     /locus_tag="VFY96_06410"
     CDS             complement(2579..2908)
                     /locus_tag="VFY96_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5606126.1"
                     /translation="MIQCTYPRNDPSIRRITPTGRVCSRNELRIIAEHCLQHDVIAIT
                     DEVYEHILYDDAVHTRLASLPGMSERTLTISSLGKTFSVTGWKVGWALGPARLVNAVN
                     QAHQFIT"
     gene            complement(2860..3360)
                     /locus_tag="VFY96_06415"
     CDS             complement(2860..3360)
                     /locus_tag="VFY96_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5606127.1"
                     /translation="MAKRVAEFGTTVFGEINELARRHKAVNLGQGAPDFDGPPEVLGA
                     AVRAVNGALNQYAPGIGMLSVREAIARHAERFYGHQLDPESEVLVTSGATEGMFAAIL
                     GLTDPGDEVIVFEPVYDTYVPNLTIAGVTPRYVPLRGDHWSFDPDELAKSFNARTRAM
                     IHQYAA"
     gene            3548..4234
                     /locus_tag="VFY96_06420"
     CDS             3548..4234
                     /locus_tag="VFY96_06420"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HEX5606128.1"
                     /translation="MSELAEEITKLKDGDHLCLFYHKDPVEQMPALIPFIQEALAKNE
                     QFIYIADDLTVDELVSHLQVGGIPVGEYVDRGTLKLWTRREWRQPGKLSSEKKSRQVL
                     DFMKAAADDGFARVRFGVEMTWTLGADIDPADLEHWEASINELFTPGSHRRIICQYNR
                     SGLSPEALLVALHTHPLTIWGNHVYLNWFYEAPLILNCPRATGERVSFLPRAFYQTYE
                     ANRSLPESTS"
     gene            4231..>5717
                     /locus_tag="VFY96_06425"
     CDS             4231..>5717
                     /locus_tag="VFY96_06425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5606129.1"
                     /translation="MMSMLERSRAAHQEHLELIEKRAALVQAEIAKKKVESVLSLMPA
                     AVYTCDLDGHITYCNQHAVELWGRAPKLDNDEDKYCGSFRLILPDGSSLARDQCRMAV
                     AVKTGETFRNEEITVERPNGSRILVRMNISPFYDEHGQLAGAINTFQDVSHLKKAEQA
                     SLHLAALVESSDDAIVAKDLDGIVKSWNPAAERLFGYTAAEIVGKPITTIIPPDRSDE
                     ESRILEQIRRGERIEHYDTVRQRKDGSPVEISLTISPIKDAHGKIIGASKIAREITKR
                     KQAETALREARDELARANQELEKRVKERTAELERANTALLKDLEEQRRLEAQLRQAQK
                     MESIGTLAGGIAHDFNNVLNIIKGYASSVASLASGNEEMTEQLEVIDDAIERGTGVVR
                     QLLTLARKTESRLISSSANELISGLTNLLKQTLPKTIDLSLELRVKLPPVMADPNQIT
                     QALLNLCVNARDAMPQGGRLTLRTSVVDGATINDAGAQAREYVCI"
BASE COUNT         1290 a         1611 c         1563 g         1253 t
ORIGIN      
        1 ccgcgccagc ctcgattccc gctaaaaacc tgcgggaatg acggaataga attcaccatc
       61 caaagcaaag ttaaaaatct agttcaaaat tcgtggtccg aacagtttga tcgcgttgtc
      121 ccagaggatt ttgcgtttcg cctcgttggg tatcggctga gcatcgagat ccggcagcgc
      181 tcgtttcgga tcgatcgcat ccgggtgcgg gtaatcggta ttccacacga tattctcccc
      241 gtttaaatct tccaccacgt atttgagcgc gcgctcatcg ctatcgcagg aaatcacgca
      301 ctggcgcatg aagtactcgc tcggcagcat gcgcagtttt tccgattttc ccatgacgct
      361 attgcgccca tgcgtgtgat cgtccattcg cccgagccaa aacggcagcc aaccggcgtt
      421 ggattcgaga attgcgaggc gcagcttggg aaagcgctcg agaatcccgg tgaaaataaa
      481 atgtcccaag gtggtcatgt tgtcgcaggg aaaacccagg gcgtgatcca gcccgcgcag
      541 cgcgagctcc gtatcacccc gacgctggcc ttcgagctgg cgaaacggat gaaagcgacg
      601 ctggcgatac tcgccatgag ctgaaatcgg aaaatccagc tcggaagcga gctgccatag
      661 cgcatcgtac tccggctcgt gcaaatagcg gccgtcgggg agaaaaggat ttcgcaccga
      721 gactgcgccg agctcggtga ctgcgcggcg cgcctctttc accatttcac gaatgtccga
      781 gccgggcagc acggcaacga acttgagccg cttcgggtcc gcgctgcagt aatcatgaca
      841 ccaattattg taggcccggc acatcgcggc gccgagttcg acatctttca aggcgacgcg
      901 ataggcgaag ttcccattgt tcccggtggg aagcagcact tggatatccc agccgtagcg
      961 gtccatgtcc ttgagccgaa tctccgacga ccaccactgc cgataggctt cgccgtactt
     1021 ttcttccaat accgcccagg cgctcggcgg cggtcgcaat tgcagatcgc ccttgggaat
     1081 gatcttgccg tccggctcat aaaccatgaa cacgccgtcc ataaaagcaa ccttgggcac
     1141 gtgatcacga aatttcggct cgacgtagcg ctcccacaaa tattggggct cgtgcatgtg
     1201 cgcgtcggca tcgatgactt tcattccgtt ccacatgagc ggctctcctc cagagtgaaa
     1261 tcaaaaccga cgttagaaga ttccggatcg aagtgtcaag cgggagtcgt gttaggtgat
     1321 tccttcaaca atcgccattt cttgcgcgcg agcggcagcc ggagcgcacg ccggtcggaa
     1381 tccgttcagc gccccagttg catcggcggg tttgtgagcc gctcggtcga aaaaaccttt
     1441 gatggttttt ccaagacgaa gtacagcggc ttgagcctac agcaattcac aattctgccg
     1501 agaatgagat aaattgatgg cgtttcgacg tggtgtcgat tgagagcaaa cacatttctg
     1561 atcgtccttt gcatagctac gagtttgtgg gcgtggcagc aaaaccccac cttcgcccag
     1621 cagaatcttg tcttcagcta caacaatcta ctgcagcgca gggtttggac cttggtcatg
     1681 gccctcttcg tccatgccaa cctgctgcac ctcatcggca acatgctttt tttgtttatt
     1741 ttcggcaata ctctggagaa aagggccggc gctcgcgcct acttggcggc gttcttttgc
     1801 ggtggattca gcagttttct tttgagcccg ctgtttatgc cgcatcgcgc gggaatgctc
     1861 ggcgcttcag cggcgatttt caccgtggcg gcctgcgtca tgctcattca gccgctaaaa
     1921 ttttcctggc tttttttggc gccccagggt ttggtggcgc tgatttattt tatttacaac
     1981 ctcgtcgtcg tctctcatcc gagcttggtt cgcggctatg atcctcatgt ggcttacatc
     2041 gctcatatca tcggttttct cgttggcatt cctttcggcg tagccttgag tcgtcagtgg
     2101 aaaagaaatc ttctgctcac gcttttgctt ttcgcgatct atttggtcat tttgcacgcc
     2161 gcccgagatt tgtttctccg gcgttgaacc ggatcacgcc cggccgagtc gacgcaagcg
     2221 ctcagctgcc gcggcgaggg tgtcgtcctt tttacaaacg gcaaaacggg cgcagtgctt
     2281 tcccaggtgc ttgtcggatc ctggtaaaac ggactcgcgg gaatgcacgc cacgccgacc
     2341 tcggcggtga gccagcgcgc gaattcatag tcgtcctgta cgcgtacggg cgcgactccg
     2401 cgccagtcaa ccattacgaa gaaactgccg ctcggctgga aaactttgaa gccggatttg
     2461 cgagcgcctc gatcagccga tcgcgccgct tttgataatc ggtttggagc tgctcgaaaa
     2521 atgacaaggg cagagctagc gccgttgccg cagccgcttg cagcggcgac gcgacggcct
     2581 aggtgataaa ttgatgcgct tgattgaccg cgttcaccaa ccgcgccgga ccgagcgccc
     2641 agccaacttt ccacccagtc acgctaaacg tctttcccag gctgctgatc gtcagcgtgc
     2701 gctcgctcat gcccggcaaa ctcgccagtc gggtgtgaac cgcgtcatca tagagaatat
     2761 gctcgtaaac ttcgtccgtg atcgcgatca catcgtgttg taagcaatgc tcggcaatga
     2821 tgcggagctc gttacgcgaa caaactctac cggtaggggt tatgcggcgt attgatggat
     2881 cattgcgcgg gtacgtgcat tgaatgattt cgccagctca tcggggtcga agctccagtg
     2941 atcaccgcga agcggcacat accggggcgt tacgccggcg atagttaggt ttggcacata
     3001 agtatcgtaa accggctcga aaacaatgac ttcgtcgcca ggatccgtaa ggcccaaaat
     3061 cgccgcaaac attccttcgg tcgcgccgct ggtaaccagg acttcactct ccggatccag
     3121 ctggtgacca taaaagcgtt ccgcgtgtcg ggcgattgcc tcccgtacgg agagcatgcc
     3181 gataccgggc gcatattgat tgagggcgcc gttgactgcg cgtacggctg cgcccagaac
     3241 ttccggcggg ccgtcgaaat ccggtgcgcc ttggccgaga ttgacggcct tgtgacggcg
     3301 cgcaagttcg ttaatctcgc caaaaactgt cgtgccaaac tccgctacgc gtttcgccat
     3361 cgcgccccgc agaccttcgt tcattagtat gctgttagga acttttgata atctcgaatc
     3421 gcacaatgaa gaaaacattg tagattttca acaaaacccg caaacgacag cgcggaaacc
     3481 tattgattga caaaacaaac catatatcct tgaagtgcgg ggttacggtc agtcccgagg
     3541 taaagaagtg tccgagctag cagaagaaat caccaagttg aaagacggag atcatctctg
     3601 ccttttttat cacaaagacc ccgttgaaca gatgccggcg ctgatcccgt ttattcagga
     3661 ggcgcttgcc aaaaacgaac agtttatcta tatcgccgat gatctgaccg tagacgaatt
     3721 ggtcagccac ttacaagtcg gcggcatccc tgtcggcgag tatgtggatc gcggcacgtt
     3781 gaagctttgg actcgtcgcg aatggcgcca gcccggaaag ctttcgtcag agaaaaaatc
     3841 acgtcaggta ctcgatttca tgaaggccgc tgcggacgat ggttttgccc gcgtgcgctt
     3901 tggcgtggaa atgacctgga cgctaggtgc ggacatcgat ccagccgatc tggagcactg
     3961 ggaagcgagc atcaacgagc tatttactcc cggttcgcac cgccggatca tctgccagta
     4021 caatcgaagc ggtctctcgc ccgaggcttt gcttgtcgca ttgcacaccc atccgttgac
     4081 gatctggggc aatcacgtct atctcaactg gttttatgaa gcgccactga ttctgaactg
     4141 tccacgggca acaggcgaac gcgtttcttt tttgccgcgc gcgttttatc aaacctatga
     4201 ggcaaatcgc tcgctgccag agtcgacttc atgatgtcta tgttggaacg cagccgggcg
     4261 gcacaccagg aacatctcga gctgatcgaa aaacgcgccg cactcgtcca agccgagatc
     4321 gccaagaaaa aagtcgaaag cgtgctctcg ctcatgcccg cggcggttta cacctgcgat
     4381 ctggacgggc acatcaccta ttgtaatcag catgcggttg agctctgggg tcgagcgccc
     4441 aagttggata atgatgagga taagtattgt ggctcgtttc gcctgatttt gccggatgga
     4501 tcgagcctgg cgcgcgacca atgtcgcatg gcggtcgcgg ttaaaaccgg agagacattt
     4561 cgtaacgagg agattacggt tgagcgaccg aatggttctc ggatcttggt tcgaatgaac
     4621 atcagccctt tttatgatga gcatggacaa ctagccggcg ctatcaatac attccaggat
     4681 gtcagccatc tgaaaaaagc cgagcaggcc agccttcatt tggcagcgct cgtcgaatca
     4741 tccgatgacg ccatcgtcgc caaagacctc gacggcatcg tcaagagctg gaatccggcc
     4801 gcggagcgtc tcttcggcta taccgcagcg gaaatcgtcg ggaagccgat taccaccatt
     4861 atcccgccgg atcgaagcga cgaggaaagc aggattctgg agcagattcg gcgtggcgag
     4921 cgcattgaac attacgatac ggtgcggcag cgcaaggacg gcagcccagt ggaaatctcg
     4981 ctcacgatct cgccgattaa agatgcgcac ggcaaaatta tcggcgcctc gaaaatcgcc
     5041 cgcgagatca ccaagcgcaa gcaggccgag acggctctac gggaagccag agacgaattg
     5101 gcgagagcta accaggagct tgaaaaacgg gttaaagagc ggaccgccga actcgagcga
     5161 gccaatacag cactgctcaa agatctcgaa gaacaaagaa ggctggaagc gcaactgcgc
     5221 caggcgcaaa aaatggaaag tatcgggact ctggccggcg gcatcgcgca cgacttcaac
     5281 aacgtcctca acatcattaa aggatatgcc agctcggtcg caagcctcgc ttcgggaaac
     5341 gaagaaatga ccgaacagct cgaggtcatc gatgatgcga tcgagcgcgg cacaggcgtg
     5401 gtgcgccagc tactcacgtt ggcgcgcaag accgaatcgc gcttgatctc cagcagtgcc
     5461 aatgagctta tttccggcct caccaatttg ctcaaacaga cgctgccaaa aaccatcgat
     5521 ctatctttgg agctgcgcgt caagcttccg ccggtcatgg ccgatccgaa tcagatcact
     5581 caggctttgc tcaatctttg cgtgaatgcc cgcgacgcga tgccccaagg cggtagactc
     5641 accttgagaa cttccgtagt cgatggcgct acaatcaacg acgctggcgc tcaggcgcgc
     5701 gagtatgtct gcatcga
//
LOCUS       DASULZ010000307         2153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1458759, whole genome shotgun sequence.
ACCESSION   DASULZ010000307 DASULZ010000000
VERSION     DASULZ010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458759"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="VFY96_06430"
     CDS             <1..797
                     /locus_tag="VFY96_06430"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5606130.1"
                     /translation="LGVATQVIFPTLFLIYITDDSTLDIALARAYNRFLAQACAKSSG
                     RMRWVVVPPLHSVDESVREIKWGKDNGAVGVFFRGIEGQRTLDNPYFNPIYQVASDTE
                     LPILIHTGAGCPLFLNLFDVERNHTFGHSRVQPLFAFRDLIHNKIPEQFPKLKFGFIE
                     ASAGWVPFMMHIIKRLFREKWKFSSDKDMFSEYRIFVACEADEDVKYISEYTGEDHLI
                     IGSDYGHQDPSEERQLVAAMRAREDVPQALTDKIFFENPKLLYPLN"
     gene            1017..2030
                     /locus_tag="VFY96_06435"
     CDS             1017..2030
                     /locus_tag="VFY96_06435"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606131.1"
                     /translation="MNNNRLSPIHQGITYQTIIAVIVSLAIALPTLTAAQSLQIANMA
                     VPAKSFQMVIYPVAQQKGYMKDEGVDERVIFVAPTTSIQAMLGGDVHFTGAGSSALVS
                     IARGNTPLKVVVATNDRVLQWLVTRPEITSAKQLKGKKIATTGVAAIATFMLKQILSK
                     HGLDANKDVNYIAVEQGTHLPALLGGGFDAAVLSVEQRYVAVDKGMHEMFFMGNEVKN
                     SWGTLATTDKLIKENPKMVAGFVRATLKALRYIRQDKEGTVAALLKFSGVSRQQASRL
                     YDDLIGTFTRNGAVDEETQKNDLNIVRQVTNSSDILPNARAYDFSFALEADQQLNKAG
                     WKP"
BASE COUNT          537 a          559 c          588 g          469 t
ORIGIN      
        1 agctcggggt ggccacgcag gtcatttttc cgaccctgtt tctgatttat atcaccgacg
       61 actcgacact cgacatcgca ctggcgcgcg cgtacaaccg atttctcgct caagcctgcg
      121 caaaatcgag cgggcgcatg cgctgggtcg tagtgccgcc gctccactct gtcgatgaat
      181 cggtcagaga aatcaaatgg ggcaaagaca acggcgcagt gggtgttttc tttcgcggca
      241 tcgagggaca aaggacgctg gacaacccgt atttcaaccc tatctatcaa gtggccagtg
      301 acacggagtt gccgattctg atccacacgg gagctggctg tccgcttttt ttgaacctct
      361 tcgatgtgga acgaaatcac acgttcggcc atagccgcgt tcagccgctg tttgcatttc
      421 gtgatttgat ccataacaag attccggaac agtttcccaa gctcaaattc gggtttatcg
      481 aggcatcggc gggttgggtg ccgttcatga tgcacatcat caaacggttg tttcgggaga
      541 agtggaagtt ttcttcggac aaggacatgt ttagcgaata cagaatcttc gttgcgtgcg
      601 aagcggatga agatgtcaaa tacatttccg agtacacggg cgaagatcac ctgatcatcg
      661 gctcggatta cggccatcag gatccttcgg aggagcgcca attggtcgct gccatgcgcg
      721 cgcgggaaga tgttccgcaa gcgctgaccg acaagatctt tttcgagaat cccaagctgc
      781 tctatccgtt gaactagcgg agctcgaggc tcgatgcgcc agcggttgtg ctagttgtga
      841 tgaacaaatc gccgcgagcc ataataccta ggtgtcatgc tgagcgctag cgaaacatct
      901 cgttcgatca gccatatccg taggcgagat tcctcggcgg agtttatcct aagtaaaatc
      961 gaagggcctc agactgacat tatgagggtg acgctgctca tcaacaggag cgcgcaatga
     1021 acaacaatcg actctcgccc atacaccaag gcataacata tcaaacaatt attgcggtca
     1081 ttgtctctct agccatcgct cttcccacgc tcaccgccgc tcaatcgctg caaatcgcca
     1141 acatggcggt gccggcaaaa agctttcaga tggtgatcta tccggttgcg cagcagaaag
     1201 gctacatgaa ggacgaaggg gtggatgagc gcgtcatctt cgtcgcgccg actaccagca
     1261 tccaagcgat gcttggcggc gatgtgcact ttactggcgc cggctcgagc gcgctggtca
     1321 gcatcgcgcg tggcaatacg ccgctgaaag tcgtcgtggc taccaacgat cgagtgctgc
     1381 aatggctggt cactcggccg gagatcacga gtgccaagca gctcaaaggc aagaagatcg
     1441 cgactaccgg cgtcgcggcg attgccactt tcatgttgaa gcaaattctt tccaagcacg
     1501 ggctcgacgc taacaaggac gtaaactaca tcgctgttga gcagggcact cacctgccgg
     1561 ctttgctcgg tggtggcttc gacgcggcgg tgctcagcgt cgaacagcgc tacgtcgccg
     1621 tcgataaggg catgcacgaa atgtttttca tgggtaacga agtaaagaat tcctggggta
     1681 cgctcgcgac gacggacaag ctgatcaaag aaaatccgaa aatggtcgcc ggcttcgtgc
     1741 gcgcaacact caaggcgctg cgctacattc ggcaagacaa agaaggcacg gttgcagcgc
     1801 tgctaaaatt ttccggagtc tcgcggcagc aagcatcgcg cctatacgac gatctgatcg
     1861 ggacgttcac gcgcaatggc gcggttgatg aagagactca gaaaaacgat ctgaacattg
     1921 tgcggcaagt tactaacagc agcgacattt tgccaaacgc gcgagcctac gatttcagct
     1981 ttgcactgga agctgatcag caattgaaca aagcaggctg gaaaccgtag aagagtgaat
     2041 tagaaaagaa cgaagtgccg agatctgcga ttactagatc taacaagatt tgaaagtcgc
     2101 aggaccgttt tagaagagat tttaaaaatg ctgaatccta tgatctctca gca
//
LOCUS       DASULZ010000308         3827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_546488, whole genome shotgun sequence.
ACCESSION   DASULZ010000308 DASULZ010000000
VERSION     DASULZ010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546488"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VFY96_06440"
     CDS             complement(<1..591)
                     /locus_tag="VFY96_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX5606132.1"
                     /translation="MIKIWGRTDSSNVQKVLWCCGELDIEFERVDWGGKFGGNNDEVY
                     REMNPNGLVPTIKDGDFILWESNSIMRYLNAKYGSGKLLPASPDGMANANRWMDWQLS
                     TFNATIVPLFWNLVRTPADKRDPKAVQSALEKTSKAWQLVEKQLAQNRFLAGADFSLG
                     DMPLGVWAYRWFSLPIERPEQPNLSRWYEQLKQRKPY"
     gene            740..1111
                     /locus_tag="VFY96_06445"
     CDS             740..1111
                     /locus_tag="VFY96_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5606133.1"
                     /translation="MNTAELLATISLLAGLMAPSTGWTQDPPEGKKLYVTYCATCHGE
                     SGKGDGAAGVSLPVRPADHTNGAAMNQLTDKFLMDIISKGGGAVGKSTFMPAWGGALN
                     EKQIRNIVSYIRSIAVPPYKP"
     gene            1244..1534
                     /gene="groES"
                     /locus_tag="VFY96_06450"
     CDS             1244..1534
                     /gene="groES"
                     /locus_tag="VFY96_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX5606134.1"
                     /translation="MATKIRPLHDRVIVKRIEEEEKTKGGIIIPDTAKEKPQEGKVVA
                     VGPGKYEDGKLIPLGVKSGDKILFGKYSGTEIKLDGEEHLIMREDDILGVVE"
     gene            1549..3171
                     /gene="groL"
                     /locus_tag="VFY96_06455"
     CDS             1549..3171
                     /gene="groL"
                     /locus_tag="VFY96_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528688.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX5606135.1"
                     /translation="MAAKEIRFSEEARGRVLRGVNLLADAVTVTLGPKGRNVVLEKSW
                     GSPTVTKDGVSVAKEIQLEDKFENMGAQMVKEVASKTSDVAGDGTTTATVLARAIFSE
                     GLKLVAAGHDPMSIKRGIDKAVAKVVDELKSMSKPTRDRDEIAQVGTISANNDKTIGD
                     ILAEAMDKVGKEGVITVEEAKGLETTLDLVEGMRFDRGYLSPYFVTDPERMECVYEDC
                     YLLIHEKKISSMKDLLPVLEAVAKSGKPLLIIAEEVEGEALATLVVNKIRGTLKVVSV
                     KAPGFGDRRKAMLEDIAILTDGKMIAEELGIKLENVTVKDLGRAKRVIIDKDNTTVVE
                     GAGKKSAIEGRIAQIRAQIDETTSDYDREKLQERLAKLAGGVAVVKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGIVPGGGVALVRAAATLANMRVSDDEKVGVRIIQKALEEPL
                     RWISQNAGMEGSVVLDRVKNGKGALGFNAATEEYEDLVKAGIVDPTKVVRTALQNAAS
                     VAGMMITTEAMIADKPEKKKEAPAMPHEHEDY"
     gene            complement(3324..>3827)
                     /locus_tag="VFY96_06460"
     CDS             complement(3324..>3827)
                     /locus_tag="VFY96_06460"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX5606136.1"
                     /translation="LDSIWGDVYVSAKRYPREDFMDLVRWEPFEGLSRIQNRINDLFD
                     DTFGRARASMPATGVWFPPVDILESRDSYLIRAELPGMKREDFNLEVHDGSLTISGER
                     KFEEAANGVEYHRVERASGKFSRSFYLPQTVKTDAIKATYRDGILEIHVPKADEAKPK
                     QITISVK"
BASE COUNT          949 a          955 c         1083 g          840 t
ORIGIN      
        1 atacggcttg cgctgcttca gctgctcata ccagcgcgac agattgggct gctccggtcg
       61 ttcgatcggc aaactgaacc aacggtaagc ccacactccg agaggcatat ctccgaggga
      121 aaagtctgcg ccggcaagga atcggttctg ggccaactgc ttttcgacca gctgccacgc
      181 cttgctggtt ttttccagtg cggactggac cgctttggga tcccgtttgt cggcaggcgt
      241 ccgcaccaaa ttccagaaca gcggtacaat cgttgcgttg aatgtagata attgccagtc
      301 catccaccgg tttgcgtttg ccatcccgtc ggggctcgcc ggcagaagct ttccgctgcc
      361 atatttggca ttcaagtagc gcatgatgct gttggattcc cacaggataa agtcgccgtc
      421 ctttatggtc ggtacgaggc cattggggtt catttcccgg tagacttcat cattatttcc
      481 accaaacttt ccaccccaat cgacgcgctc gaactctata tcgagttcgc cgcagcacca
      541 gagtaccttt tggacattcg aggaatccgt tctgccccag attttgatca tgctcgcccc
      601 catcgtgcaa aattgttggc tagatttatc atcgtttcgc aaatttgact acagcgcaca
      661 gtaagtcgta tagcggcggt tgtcgttgcc gagaaatatc tgctaggaaa agaccaacaa
      721 ctttaaaagg cggaggcaaa tgaacactgc agagttgttg gcgaccatat cccttttggc
      781 aggtttgatg gcgccgtcta ctgggtggac gcaggacccg ccagagggta aaaagctcta
      841 cgtgacttac tgcgctacat gccacggcga aagcggcaaa ggcgacggcg cggccggtgt
      901 ctccctcccg gtacgaccag cggatcatac caacggcgcc gccatgaacc agctcactga
      961 caagtttttg atggatatca tctccaaagg cggcggcgcc gtcggaaaat cgacatttat
     1021 gccggcttgg ggcggcgcgc tgaacgagaa gcaaatccgc aatattgtct catacattcg
     1081 atcgatcgcg gtcccccctt ataagccgta gtaaaatcca cccgaaagtg taaatttttt
     1141 tcggttatac cccttgacga ttcgctcagc gagattaaat tagcccgcat ccaacctgtt
     1201 ctgcaggatc taaatttatg ttttatttca ggaggttttt ataatggcga ccaagattcg
     1261 tcccctacac gatagggtaa ttgtgaaacg gattgaggag gaagagaaaa ccaagggagg
     1321 tatcatcatc ccggatacag ccaaggaaaa accgcaagag ggtaaagtgg tcgcggtggg
     1381 tccaggcaaa tacgaagacg ggaagctcat acctcttggg gtaaagtctg gcgataagat
     1441 tctatttgga aaatattcgg gcacagagat caagttggac ggcgaggagc atctgattat
     1501 gcgcgaagat gacattcttg gcgtcgtgga ataaggagga gacagaagat ggctgcgaag
     1561 gaaatacggt ttagtgaaga ggcgcggggt cgggttctcc gcggtgtaaa tctgctcgcc
     1621 gatgcggtga cggtgacttt agggcccaaa gggcgaaatg tcgtcctcga aaaatcctgg
     1681 ggctccccga cggtcacgaa agacggtgtc tccgtggcga aggaaatcca gctggaagac
     1741 aaattcgaga atatgggtgc acaaatggtc aaagaagttg ccagtaaaac ttcggatgtc
     1801 gctggcgacg gcaccactac ggcaaccgta ttggctcggg cgattttcag cgagggacta
     1861 aagttggttg cggctggcca tgacccgatg agcattaagc gcggcatcga taaagcggtc
     1921 gcgaaagtcg tcgacgaact caaatccatg tctaaaccca cccgcgatcg ggacgaaatc
     1981 gcgcaagtcg gcaccatatc cgcgaacaat gacaaaacca tcggcgatat tctcgccgag
     2041 gcgatggaca aggtcggaaa agaaggcgtt atcacagtcg aggaagcgaa ggggttggaa
     2101 acaacgctcg acctcgtcga aggaatgcgg ttcgatcgcg gctatctctc tccctacttt
     2161 gttaccgatc ctgagcgcat ggaatgcgta tacgaagact gttatctgct gattcacgag
     2221 aaaaagattt ccagcatgaa agacctgctg ccggtgctcg aagcggtagc caaaagcggc
     2281 aagcccctgc tcatcatcgc cgaagaggtc gaaggcgaag cgctggcaac tctcgtcgtg
     2341 aacaagatcc gcggcacgct gaaagtcgtc tccgtcaaag ctccgggttt cggcgaccgg
     2401 cgcaaggcga tgctcgaaga catcgctatc cttaccgatg gcaagatgat tgccgaggag
     2461 ctcggcatca agctggaaaa cgtcacggtg aaagatcttg gccgggcgaa acgagtgata
     2521 atcgacaaag acaacactac ggtggtcgaa ggcgcgggta agaaatccgc catcgagggg
     2581 cgtatcgccc agatccgggc gcagatcgat gagaccactt cggattatga ccgtgagaaa
     2641 ctgcaggaaa ggctggccaa gctcgccggc ggcgtcgccg tcgtcaaggt tggcgcggcg
     2701 acggaagttg agatgaagga aaaaaaggcg cgcgttgaag atgctctgca cgcaacccgg
     2761 gcggcggtcg aagagggtat tgtgcctggt ggtggcgtcg cgctggttcg cgcggcggcg
     2821 accttggcga atatgagagt ttcggatgat gagaaggttg gtgtccggat catccagaag
     2881 gcgttggagg aaccacttcg ttggatctcc caaaacgccg ggatggaagg ctcggtggta
     2941 ttggatagag taaagaacgg taaaggcgcg ttaggtttca atgctgcaac tgaagagtac
     3001 gaagatctgg tcaaggccgg aatcgtggat ccgactaagg ttgttcgcac agcattgcag
     3061 aatgccgcct cagttgcagg catgatgatt acgaccgagg cgatgatcgc cgataagcca
     3121 gagaagaaga aagaagcgcc ggccatgccg catgaacacg aagactacta agcttctctc
     3181 gcgcatttaa agagggcgcc tggtaacagg cgccctcttt tgttccgcgt acgtctgatc
     3241 tcacagcagc atttgtgccg aaaattaagc gcgaaaaaag ggaggcaatt gcctcccttt
     3301 tttatgggac gagccgaact ttattatttt acgctgatgg tgatctgctt tggtttcgct
     3361 tcgtcggcct ttggtacgtg tatttctaaa atgccgtcac gataggtggc tttgatggcg
     3421 tcagttttga cggtctgtgg caggtaaaag gatcgcgaga actttcccga cgcccgttcg
     3481 acgcgatggt attccacgcc gttagcggcc tcctcaaatt tgcgctcacc actaatcgtc
     3541 agtgagccat cgtgcacttc gagattgaaa tcttcgcgtt tcattcctgg aagttccgcc
     3601 ctgatcaggt aggagtctct gctctcgagg atatcgacgg gcggaaacca tacccctgtg
     3661 gcaggcattg aggcacgagc ccgcccaaag gtatcatcga acagatcatt gatgcgattt
     3721 tgaattctgc tcaatccttc gaagggttcc catctgacca agtccataaa atcctccctt
     3781 ggatatcttt tggcggaaac gtaaacatcg ccccaaatac tgtcaag
//
LOCUS       DASULZ010000309         4127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_647804, whole genome shotgun sequence.
ACCESSION   DASULZ010000309 DASULZ010000000
VERSION     DASULZ010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647804"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VFY96_06465"
     CDS             complement(<1..572)
                     /locus_tag="VFY96_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX5606137.1"
                     /translation="MKTLKLCCFISFLALALSVMAQSKDAAAPHVDLISIDGTINPAV
                     DDFIRESIGRAKASGAKALIIQLDTPGGLLNSTRTIVKEMLGAEVPLMVYVAPSGAGA
                     GSAGVFITMAAHIAAMAPGTNIGAAHPVAGAGQEVKGVMGEKIENFTASFSESIAQKR
                     GRNTEWAIQAVRKSVAITEKEALKKNVIDIV"
     gene            complement(559..1224)
                     /locus_tag="VFY96_06470"
     CDS             complement(559..1224)
                     /locus_tag="VFY96_06470"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX5606138.1"
                     /translation="MARDNRIEGNPSGKRTHSITGSGAELFSSSAPSLVASTLEELRA
                     AIGDCRRCKLCSGRTHLVFGVGNPRAELMFVGEGPGRDEDLQGEPFVGRAGQLLTDII
                     TKGIGLRREDVYIANVVKCRPPDNRNPEPDEVAACEPFLKKQIELISPAIIVALGKFA
                     VQTLLQSKMPITKLRGTWHSYQGIKLMPTFHPAYLLRNPADKKLVWEDIKKVIQEMRR
                     ENP"
     gene            complement(1419..1952)
                     /locus_tag="VFY96_06475"
     CDS             complement(1419..1952)
                     /locus_tag="VFY96_06475"
                     /inference="COORDINATES: protein motif:HMM:NF025743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HEX5606139.1"
                     /translation="MGTPREPKPAKSFVALLSSTQELLSQAEKELMALMGQIDGRSAV
                     VPWNVSKYYEREMGSGLLRCFVGFSSLLPPERLAELKLKTQVIEDEYRDSAGARQINI
                     DPGYLDAHKVVLASTKNASQRIYLRSGIYAEATLFYDNGHFHGLAYTYRDYLWPEALE
                     FFTDLRAGYLKQLKQLP"
     gene            complement(1954..3159)
                     /gene="coaBC"
                     /locus_tag="VFY96_06480"
     CDS             complement(1954..3159)
                     /gene="coaBC"
                     /locus_tag="VFY96_06480"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX5606140.1"
                     /translation="MATNGQQRMVVLGVTGGIACYKAVELVRLLVKNGFNVRVIMTRG
                     ATEFVTPLTFQTLSGNPVATEIFNLTQESEIGHINLADSADLFVIAPATANVIGKLAS
                     GIADDLLTTVLMATQAPVLLAPAMNIHMYANVIVQENLRKLRRVGYHIMDPAEGYLAC
                     GYEGKGRLPDPEKISDEIERLLRKKDLAGENLLITAGPSREPLDPVRYISNRSSGKMG
                     YALARAALRRGATVALVSGPTALDAPPGARLIEVSTAAEMREAVLKEYSRSSAVIMAA
                     AVADYHAAAPSGEKIKRGVGAIELRLVPNPDILKELGENKDGKWLVGFAAETENLIAN
                     AQKKLRDKNLDIVIANNVAEEGSGFDGDTNVATIIDRSGAVRSLPLMSKDDLADCIYD
                     HYLALKKRS"
     gene            3443..>4127
                     /locus_tag="VFY96_06485"
     CDS             3443..>4127
                     /locus_tag="VFY96_06485"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEX5606141.1"
                     /translation="MMKREKKLWTGAVPAVAFGMWLGFAVLIQTIVSADESSAAAQAS
                     EKANQITLAQAETQPSAAIAAAVSLAGMAASSSAGEDFEDDPFEGFNEKMFWFNREVL
                     DRYVLKPIATGWDFILPDPVQRGVHNFFDNLAVVRRVVNNSLQLKFNGAATELARFTI
                     NSTVGVVGFFDVAKDAFGIEQRDEDTGQTFGVWGAGPGPYLVLPFLPPLTIRDGIGYA
                     FDVAMTPYTY"
BASE COUNT          899 a         1143 c         1106 g          979 t
ORIGIN      
        1 acgatgtcga tgacgttttt cttgagcgct tctttttccg taatggcgac gcttttgcgc
       61 actgcctgga tcgcccattc cgtattgcgc ccgcgttttt gcgcgatact ttcgctaaat
      121 gatgcggtga agttctcgat cttctcaccc atgacgcctt tgacttcctg gccggcaccg
      181 gctactggat gcgccgctcc gatattggtt cccggagcca ttgcggcaat gtgcgctgcc
      241 atggtgatga aaacgccggc cgatccagca ccggctccgc tcggcgccac ataaaccatg
      301 agtggcacct cggctccgag catctctttg acaatggtac gggttgaatt gagcaatccg
      361 cccggtgtgt ccaactgaat gatcaatgcc ttcgctccgc tggcctttgc ccgtccaata
      421 ctctcgcgaa tgaaatcatc gactgcagga ttgatggtcc cgtcgatgga aatcagatcc
      481 acatgcggtg cggcggcatc cttactttgc gccatcaccg aaagcgccag tgccaaaaat
      541 gaaatgaagc aacacagctt aagggttttc acggcgcatc tcttgaatca ccttcttaat
      601 atcttcccaa accagctttt tatccgccgg attgcgcagc aaataagcgg ggtgaaatgt
      661 cggcatcaac ttgatcccct gataactatg ccaggtaccg cgcaatttcg tgatcggcat
      721 cttgctttgc agaagcgtct gaacggcgaa cttgcccaaa gccacgatga ttgctggact
      781 gatgagctca atttgttttt tcagaaatgg ctcgcaggcc gcgacttcgt caggttcagg
      841 attgcggttg tccggcggtc ggcacttgac cacatttgcg atatagacgt cctcgcgtct
      901 gagcccgata cccttggtga taatatcggt caaaagttgt cctgctcgtc cgacaaacgg
      961 ctcgccctgg agatcttcgt cgcgcccggg cccctcgccg acaaacatca gctcggcgcg
     1021 cggatttccc acgccgaaaa ccaaatgggt acggcccgag cataatttgc agcgacgaca
     1081 atcgccaatt gcggcgcgca actcttcgag agtactcgcg acaagcgacg gcgcgctgga
     1141 agagaacaac tcagcgccac tgccggtgat ggaatgtgtc cttttccccg aagggttacc
     1201 ctcgattcga ttgtctctag ccactctcgc tatttccacc tgcgctcggc gctgtaaatg
     1261 gccacgcagc gagctcgtta acaacagcaa ttcttctgcg tagttcttgt ctgacattgg
     1321 ctgctttatt taacccggca tgatcttagc accggcgact tgcacctagc aacgctacgg
     1381 cgttacgggt gtgcggacga gaccgttgct cgggcagact acgggagctg cttcagttgt
     1441 ttcaagtaac cggcacgaag atcggtaaaa aactccagtg cttccggcca aagatagtct
     1501 cgatatgtat aagctaaacc gtggaaatgt ccattgtcat agaacaaggt cgcttcggcg
     1561 taaatgccag aacgcaaata aatcctctgg ctggcatttt tggtggatgc gagaacgact
     1621 ttgtgcgcgt cgagatagcc gggatcgatg ttgatctgac gcgcgccggc gctatcgcga
     1681 tattcgtctt cgatcacttg cgttttgagc ttcaactccg ccagccgttc gggcggcagg
     1741 agagatgaaa atccgacgaa acacctcaac aatcctgaac ccatttcccg ctcgtagtat
     1801 ttggagacat tccaaggcac cacggcgcta cggccgtcga tctggcccat cagcgccatt
     1861 agctcttttt cagcttgcga gagaagttcc tgcgtggagg agagaagcgc gacgaaagat
     1921 ttcgccggct tcggctcccg tggagtgccc atttcagctg cgcttcttca aagcgaggta
     1981 gtggtcgtag atacaatcgg cgagatcatc cttgctcatc agcggcagcg agcgaacggc
     2041 gccggagcgg tcaataattg tcgccacatt ggtatcgccg tcaaaaccgc tcccctcttc
     2101 cgcaacattg tttgcgatga cgatatccag atttttgtcg cgcagtttct tttgcgcgtt
     2161 ggcgatgaga ttttcggtct ccgcggcaaa accgaccagc catttgccgt ctttattctc
     2221 tcctagttct ttcaagatat ccggattggg tacgagccga agctcgatgg cgcctacacc
     2281 gcgctttatt ttctcgccgc tcggcgccgc ggcgtgatag tcggcaaccg ccgctgccat
     2341 gattacggcg ctcgagcgtg aatattcctt aagaactgcc tcgcgcattt ccgctgcggt
     2401 gctcacctcg atgagacgag cacccggcgg tgcatcaaga gccgtgggcc cgctaaccaa
     2461 tgctactgtc gcgccgcgtc gtagcgcggc gcgcgccaac gcatatccca ttttgcccga
     2521 cgatcggttt gagatgtaac gaaccggatc gagcggttcc cggctcggac ccgcagtaat
     2581 caacaaattt tcgccggcaa ggtccttttt gcgcagtagc cgttcgatct cgtcactgat
     2641 cttttctggg tcgggcaggc gccctttgcc ttcgtatcca caagctagat agccctcggc
     2701 cgggtccata atatgataac cgacccgccg gagcttacgt aggttctctt gaacgatcac
     2761 gttcgcgtac atatgaatgt tcattgctgg cgcgagcagc acgggtgctt gagtcgccat
     2821 aagcacggtc gtcaataagt catcagcgat gccactggcg agtttcccga tcacgtttgc
     2881 cgttgccggc gcaatcacaa agagatcggc ggaatccgcg agattgatgt ggccaatctc
     2941 ggattcttgc gtcaggttaa aaatttctgt agcaacggga ttgcccgaga gagtctgaaa
     3001 cgtgagcggc gtcacgaatt ccgttgcgcc gcgagtcata atcacccgaa cgttgaaacc
     3061 atttttgact aaaaggcgca ccagctcgac tgctttgtaa cacgcgatgc cgcctgtgac
     3121 accgagaaca accatccgtt gttgaccgtt ggtcgccatg ccccaaaatt aaccgatcag
     3181 ccgcaggcgt gcaaacttcg ataaccacga ttagctcaag tagcgcggaa tctgccagcg
     3241 gttaccagtt tgcgtcaggc gcggttcctt tttattgcgt cgatgcgaac aagctcccgg
     3301 atgtaccaag ctggcaggag aaatgtgggg gcacgtggca tggttttcgc gttcgcttcg
     3361 agacttcaga gtcgcgacag attagcaaaa cacttgacca cgcttcagcg ttaatgtaat
     3421 cacacagacg cgagaaggag acatgatgaa gagagagaaa aagctatgga cgggagccgt
     3481 accagccgtg gcattcggta tgtggttggg gttcgccgtg ctgatacaaa caattgtgag
     3541 cgccgacgag agttccgcgg cagcgcaggc gagcgaaaaa gccaatcaga ttacacttgc
     3601 tcaagccgag actcagccct ccgcggcgat tgcggctgca gtaagtctgg ctggaatggc
     3661 ggcaagctct agtgcaggcg aagatttcga ggacgatccg tttgagggct tcaacgaaaa
     3721 aatgttctgg ttcaaccgag aagttctgga tcgttacgtt ttgaaaccga ttgccacggg
     3781 gtgggatttt atactgccgg atccagtgca acggggcgtg cacaattttt ttgacaatct
     3841 tgccgtggtt cgacgtgtcg tcaataacag cttgcagctc aagtttaatg gcgccgcgac
     3901 tgaactcgcg cgcttcacga tcaacagcac ggttggcgtt gtaggatttt tcgatgttgc
     3961 aaaagatgca tttggtatcg agcaaagaga cgaggacacg gggcaaacgt ttggcgtctg
     4021 gggagccggt cctggaccct atctggtgtt gccctttttg ccaccactca cgatccgtga
     4081 tggcatcggt tatgccttcg acgtcgccat gactccctat acctact
//
LOCUS       DASULZ010000310         2775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_952621, whole genome shotgun sequence.
ACCESSION   DASULZ010000310 DASULZ010000000
VERSION     DASULZ010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952621"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2775
                     /gene="rpoC"
                     /locus_tag="VFY96_06490"
     CDS             <1..>2775
                     /gene="rpoC"
                     /locus_tag="VFY96_06490"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX5606142.1"
                     /translation="GGKFIAEMGAEAIRKLLKAIDVAKLSEDLRQEMRDANSEVRRKK
                     MAKRLKVINAFRTSGNRPEWMVMEVIPVIPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLMELSAPDMIIRNEKRMLQEAVDALFDNGRRGRAITGQNRRPLKSLSDMLK
                     GKTGRFRQNLLGKRVDYSGRSVIVVGPELRLHQCGLPKKMALELFKPFIYNKLEERGH
                     VTTIKSAKKMVERELPEVWDILDEVIREHPVMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAYNADFDGDQMAVHVPLSVEAQVEARALMMSTNNILSPANGKPIIVPTQDI
                     VLGLYYMTRDRLHAKGSGRLFSNPSEVRVAYDQGEVDIQAPIMVRINGERIETTVGRV
                     LLYEVVPEVIPFKEINRVMKKKELANLIDVSYRLAGNKATVILADRLKDIGYQNATKA
                     GISISIKDMVIPPSKEELLKKAHDDVREIEEQYKNGLITDGERYNKVVDIWAEVTDRI
                     ADEMLRDLGSEKVTDEQGREIMTPSFNPIFMMADSGARGSAQQIRQLAGMRGLMAKPS
                     GEIIETPITANFREGLTVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVAQDAVI
                     AEEDCGTMDGIEMTPLMEGGEIIEPLGDRVLGRVALEDIKDPFTGDVIVKPNQEINEE
                     LVQRIEDAGLERIKIRSVLTCASRRGVCIMCYGRDLGRGHMINLGEAVGVIAAQSIGE
                     PGTQLTMRTFHIGGTASRRAEQTTLESRNEGTLKFINVSTVTNKDGDLVVMNRNGEVA
                     IVDYPEGEKGRERERERYPVVYGAKLKKKDSSKVKAGELLAEWDPYTIPILTEVSGVV
                     KFGDVIETVTMEEKVDERTGLSTKVITDSKDVEKRPRISIKDIEGQTARLSATSEARY
                     LLPVGAHINV"
BASE COUNT          689 a          717 c          831 g          538 t
ORIGIN      
        1 cggcggtaag tttatcgccg aaatgggcgc cgaagcgatc cggaagttac tgaaggcgat
       61 cgatgtcgcc aaactttccg aagatctgcg ccaggaaatg cgcgatgcga actccgaagt
      121 gcggcgcaag aaaatggcca aacggctaaa agtgatcaac gcatttagga catccgggaa
      181 tcgaccggag tggatggtca tggaagttat tcccgtcatt ccacccgatc ttcggccact
      241 ggttccgctc gacggcggcc gcttcgcgac gtccgatctg aacgatcttt atcgtcgagt
      301 gatcaatcga aacaaccggc tcaagcgtct gatggaattg agcgcgcctg acatgatcat
      361 tcgcaacgaa aagcggatgc tgcaggaagc ggtggatgca ttgttcgata acggcagacg
      421 cggccgtgct atcaccggtc agaatcggcg cccgctcaaa tcattgagcg atatgctcaa
      481 gggcaaaacc gggcggttcc ggcaaaactt gctgggcaag cgcgtcgatt attccggacg
      541 atcagtgatt gtcgttggac ccgagctacg tctgcatcaa tgcgggttgc caaaaaagat
      601 ggctttggag ctttttaagc catttatcta caacaagctt gaagagcgtg gccatgtgac
      661 aaccatcaag agcgcgaaaa aaatggtcga gcgcgaattg ccagaggtct gggatattct
      721 ggacgaagtg attcgagaac atccggttat gctgaatcgc gcaccgacct tgcaccggct
      781 cggtattcag gccttcgagc ccattctgat cgaaggcaaa gcgattcagc tccatccgct
      841 cgtgtgcgcc gcctacaacg cggatttcga cggcgatcaa atggcggtgc atgtgccgct
      901 ttccgtcgaa gcgcaagtgg aagcgcgggc gctgatgatg tcgacgaaca atattctctc
      961 gcccgcaaac ggcaagccga tcatcgtgcc gacccaagac atcgtgctcg ggctttacta
     1021 catgacacgc gatcggttgc acgcaaaagg cagcggtcgg cttttcagca acccgagcga
     1081 agtgcgggtc gcatacgatc aaggggaagt cgatattcag gcgccgatta tggtgcgcat
     1141 caacggcgag cgaattgaga ccacggttgg gcgggtgttg ctgtacgagg ttgtaccgga
     1201 agttatcccg ttcaaagaaa tcaatcgcgt tatgaaaaag aaagagctcg caaacttaat
     1261 cgatgtgagc taccggctgg cgggaaacaa ggctacggtc attctggccg atcggttgaa
     1321 ggacattggt tatcagaatg cgaccaaagc gggtatttcg atttcgatca aagacatggt
     1381 gattccgccg agtaaggaag agctgctcaa gaaagcgcac gacgatgtgc gtgaaatcga
     1441 ggagcagtac aagaacggct tgatcaccga tggcgaacgt tacaacaaag tcgttgatat
     1501 ttgggccgaa gtcaccgacc gtatcgccga tgaaatgttg cgcgatctcg ggtcagagaa
     1561 agtcacggat gaacaagggc gcgaaatcat gacgccgagc ttcaacccga ttttcatgat
     1621 ggccgactcg ggcgcgcggg gcagcgcgca acagattcgc cagctcgcgg gaatgcgcgg
     1681 tctcatggcg aaaccttcgg gcgaaatcat tgagaccccg atcactgcga actttcgcga
     1741 aggcttgacc gtgttgcagt acttcatttc aacccacggc gcgcgcaaag gcctcgccga
     1801 cacggcgtta aagacggcca actctggtta ccttacccgg cgactggtcg acgtggcgca
     1861 ggatgcagtc atcgccgagg aagactgcgg cactatggac ggcattgaaa tgacaccgct
     1921 catggaaggc ggcgaaatca tcgagccgct cggcgatcgc gttctcggac gcgttgccct
     1981 ggaggatatc aaagatccgt tcaccggcga tgtgatcgta aaacccaatc aggaaatcaa
     2041 tgaagagctg gttcagcgca tcgaggatgc aggcctagag cggatcaaga tccggtccgt
     2101 actcacctgt gcgtcgcgtc gcggcgtttg cattatgtgc tatgggcgcg atttagggcg
     2161 cggtcatatg atcaacttgg gtgaagcggt gggcgtcatt gcggcgcaat cgatcggcga
     2221 gcccggaacg cagcttacca tgcggacgtt ccatattggc ggtaccgcaa gccggcgcgc
     2281 ggaacagact accctggaat cgcgcaatga aggcaccctc aagttcatca acgtgagcac
     2341 cgtcacgaac aaagacggtg atctcgtcgt gatgaatcgt aacggcgagg tggccatcgt
     2401 cgattatccg gagggcgaga aggggcgcga gcgcgaacgt gagcgctacc cggttgtgta
     2461 tggcgcaaag ctcaagaaaa aggatagcag caaagtcaaa gccggcgagc tcttggcgga
     2521 atgggatccc tacaccattc cgattctcac cgaggtgagc ggtgtcgtga agttcggtga
     2581 cgtcatcgaa accgtcacca tggaggaaaa ggtcgacgag cgcaccggac tctcgacgaa
     2641 ggtcatcacc gattccaagg acgtggaaaa gcgcccgcgt atttcgatca aagacatcga
     2701 agggcaaacc gcacgtttgt cggcgacatc ggaagcgcgc tatctcttgc cggtgggagc
     2761 gcacatcaat gtgca
//
LOCUS       DASULZ010000311         2137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_851227, whole genome shotgun sequence.
ACCESSION   DASULZ010000311 DASULZ010000000
VERSION     DASULZ010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851227"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..1225
                     /locus_tag="VFY96_06495"
     CDS             242..1225
                     /locus_tag="VFY96_06495"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606143.1"
                     /translation="MDERKILCRAVIIIGLLLSLHVAHGASGPMKVTLHIPSKSLSIM
                     PYYFGKDKGFFAAEQIEPQLVMMAPPTAIAALVGGELDFSSTTGAATSAIMRGLPLRR
                     VFYVQQDPVHALMAQPEIKSVRDLVGKAVGVTALTDAVGMSANVILRANGIEAGKVTL
                     LAMQVTDNAIQSLITKRVAATLLAPPYVEELEAKGYVKLVEARTYAPLSFIGLVSSTD
                     AIKKTPQKVYGMVAALHRTMNYILNPANRAEVVQYVSKYHKIDLPLAEKAFAAQMLGY
                     SKDGTKPRAAVEKEIEIYRESLKIAKSFTPDDLEDMSFLRKVQEATDSKSR"
     gene            complement(1260..>2137)
                     /locus_tag="VFY96_06500"
     CDS             complement(1260..>2137)
                     /locus_tag="VFY96_06500"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606144.1"
                     /translation="GLAARSTTSMPFFVAKERGFFAEEGLDVELIVMQAIQTIQATMG
                     KSTQFASATGSAVSAAVSGADIKTILAVTDRPSFDLIVQPNITSVQQLRGKKIGTGGV
                     GSLAEILTRRILLVNNVRPEEVTILATGPSHVTYLSLKAKVIDAAPLQMPLTFTAQDE
                     GFRKLVAAGDVYQSVQGGLATTKLLLTEQPELVTKVVRAMLRAIRLIKSDRKYGIDFI
                     KGPWLDLGKDRERIAARVYDAAAPDLLENGVVSEDIQRQMIADAAARVKPAHPVLPEQ
                     VFDFSFTRKVAAALK"
BASE COUNT          468 a          568 c          614 g          487 t
ORIGIN      
        1 agaagtttcc gaattttgtc attccgagcg tcagactgtg tgaaaactcc gctgatgttt
       61 cggcaagctc agcatgcgcg gtttaggtgt tctgtaccga caactactta gcggttcatc
      121 ctgagcgtgt cgaaggacga acggcgagtt ttcacacgtg ctaaactcgg gccgacagtc
      181 tttgtgtcaa agttctggac aagatagctg tgagctaggg ccgtcttaga gctggggtgt
      241 gatggacgaa cggaaaattt tgtgtcgtgc ggtgattatc attgggctgt tactatcatt
      301 gcacgtcgcg cacggcgctt cgggaccgat gaaggtcacg ctccatatcc cctcgaagag
      361 cttgtccatc atgccctact attttggcaa agataaaggt ttttttgcgg cggagcagat
      421 cgagccacaa cttgtcatga tggcgccgcc taccgcgatc gcggcgctag tcgggggcga
      481 attggatttt tcttcaacga cgggagccgc cacgtcggcc atcatgcgag ggttgccgct
      541 ccgccgcgtg ttttatgttc agcaggaccc ggtgcatgcg ttgatggcgc aaccggagat
      601 caagtcggtt cgagatttgg tcggtaaggc ggtgggtgtc acggcattga ccgacgccgt
      661 gggcatgtcg gccaacgtga ttcttcgcgc caacggcatt gaagccggca aagtcacgtt
      721 gctcgcgatg caggtcaccg acaacgcgat acaatcgttg atcacgaaaa gagtggctgc
      781 gactttactg gcgccgccct atgtcgagga attggaagcg aagggttatg tcaagctggt
      841 ggaagctcga acatacgccc cattgagctt catcggtctc gtgtcgtcga ccgatgcgat
      901 caaaaaaact cctcagaagg tttatggcat ggtcgcggca ctgcaccgga cgatgaacta
      961 tattctcaat ccggcgaatc gcgccgaagt ggttcagtac gtttcgaaat atcacaagat
     1021 tgatttgccg ttggcggaaa aagcgttcgc cgcgcagatg ctcggctaca gcaaagacgg
     1081 aacaaagcca cgtgcggcgg tagaaaaaga aatcgaaatt tatcgagagt cgctaaaaat
     1141 cgctaagagt ttcacgccgg atgacttaga agacatgagc tttctcagga aggtgcagga
     1201 ggccacggac tcaaagagtc gctgactaaa tccgcgctaa tttctgtctc atcccacagc
     1261 tatttcaacg ccgccgcgac tttccgtgta aaactgaaat cgaatacctg ctcgggcaaa
     1321 accggatgcg ctggcttgac gcgcgctgcg gcgtcggcga tcatctgccg ttgaatgtct
     1381 tcactcacca cgccattttc caggagatcg ggcgcagccg cgtcgtagac acgggcggcg
     1441 atccgttccc gatctttgcc gagatctagc caggggccct tgataaagtc gatgccgtat
     1501 ttcctgtcgc ttttgatgag gcggatcgcc cggagcatcg cgcgcactac cttggtgacg
     1561 agctcgggct gctcggtcaa caaaagtttt gtcgtcgcca ggccaccttg taccgattga
     1621 tatacatcac ccgccgcgac gagcttgcga aaaccctcat cctgagcggt gaaggtcaac
     1681 ggcatttgca gcggcgcggc atcgatgacc ttcgctttca atgacaagta cgtcacgtgg
     1741 ctggggccgg tagcgagaat cgtgacttct tcggggcgaa cgttgttgac gagcaaaatt
     1801 cgtcgcgtga gaatctctgc caatgagccg accccgccgg tgccgatttt ttttccgcgc
     1861 aattgttgca cgctggtaat gttcggttgc acgataagat cgaaggacgg gcgatccgtc
     1921 accgccaaaa tggttttgat atcggcgccg ctgacagcgg cgctcaccgc tgagccggtc
     1981 gccgacgcga actgcgtgct tttgcccatc gttgcttgga tcgtttgaat cgcctgcatg
     2041 acgatcagct cgacatccaa accttcctcg gcaaaaaagc cgcgttcttt ggcgacgaaa
     2101 aacggcatcg acgtggtgct gcgcgcggcc agcccca
//
LOCUS       DASULZ010000312         2751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_851286, whole genome shotgun sequence.
ACCESSION   DASULZ010000312 DASULZ010000000
VERSION     DASULZ010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851286"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..1016
                     /locus_tag="VFY96_06505"
     CDS             201..1016
                     /locus_tag="VFY96_06505"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5606145.1"
                     /translation="MAESTQRDSALCHLRVIELGDIPASYATRWLADLGADVIKVEPP
                     GGDPNRMLAPFAGNIADSERSLAFIHANTNKRSIVLDLARETDREIFTKLLGSANLLV
                     EATPLGYLENLGLDDEVLSNKFPAMVIVSITPFGRTGPYRHYKGSDAIANATGGFLFG
                     QGDDSKGQCTAPSHLAYQMAGAMASMLGLAGLRHARITGAGQRIDMSLQEALTFANSS
                     SIARYSRENRLERRPGAKAYGGAGTNIYRCKDGRYVHFTTNMPTCGRSLRRTG"
     gene            986..2596
                     /locus_tag="VFY96_06510"
     CDS             986..2596
                     /locus_tag="VFY96_06510"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5606146.1"
                     /translation="MWKEFAQNWMTDKSLAGSEWENPRYRDEHSEEVAKAFAGFIGEF
                     EADDFANQAQARHLAAAPLNTVGQFVECEQNRAREWLQEINHPVIGKYKAPGAPMRLS
                     VTPMRVRRPAPLLDQHRKEILADLDKLPAPAIQQRKSESARQPMLAGLRMADLTQQYA
                     GPLGTELLAYYGMEVVKIETGTIPSKERQAAVHACMNRAKLGCTLNLRNPEGKELFKQ
                     LVSRSDVVVDNFSSGVLERLGFSFKVLQKINPGVVQVVMPGWGLTGPLQSWVAWGWQL
                     LAYTGIMRLWGYPDSPMETRCKIAWPDRVGSVTMALGVLAALEYQAQTGRGQFIEAGM
                     LEAQGSMMGPAILDYTVNGNDWDAMGYREILGEPYTPYGCYPCRGEDAWIIIACAGDE
                     QWQSMVKLIGANSWAADEKYASAAGRKQFRNELDQKLSEWTSSYTVRQVFRMLQESGV
                     AAGIPSSGEDLFHDFHLRARGHIVETDAQPWGRIAHHGLPGIPSLSSADAARGAPWIG
                     AHNAKVFGEILGLSRERIEELKKAEAIK"
BASE COUNT          599 a          736 c          847 g          569 t
ORIGIN      
        1 ggaagtttcc ctcgaagaat tgaatcccaa aattgacgcg atcctgaaag gcgccgtgcg
       61 cggcagggta ttgatcgatt taagccgata gttcattcac cacagggaca cggagtccac
      121 agaggcgggg tgttttgaaa ctccgcgtcc tctgtgtctc cgtggtgaaa ttgcttttgg
      181 tagttacgag aggcaatcgc ttggcagaat caactcaaag agactccgct ctctgtcatc
      241 ttcgggtaat cgaactgggc gacattccgg cttcatacgc gacccgttgg ctcgccgatc
      301 tcggcgccga tgttatcaaa gtggaaccgc ccggcggtga tcctaaccgt atgctggcgc
      361 cctttgccgg caacatcgcc gattccgagc gcagtctcgc cttcatccat gccaacacca
      421 acaagcgcag tatcgtgctc gatctcgccc gtgaaacgga tcgtgaaata ttcacgaagc
      481 tccttggctc cgccaatctc ttggtcgaag cgacgccgct tggctatttg gagaatctcg
      541 gcttggacga cgaagtcttg agcaacaagt ttccggctat ggtcatcgtt tcgataaccc
      601 cttttgggcg caccggaccc taccgtcatt acaaagggag cgatgccatc gcgaacgcca
      661 ccggcggatt tcttttcggt cagggcgatg acagcaaagg gcagtgcacg gcgccgagcc
      721 atctcgcgta tcaaatggct ggtgcgatgg cgtcgatgtt gggtttggct ggactgcgcc
      781 atgctcgcat taccggggct gggcagcgca tcgatatgtc attgcaggag gcgctgacgt
      841 ttgcgaatag cagctcgatc gcgcgctaca gccgcgaaaa tcggctggaa cgccgtccgg
      901 gggcgaaggc ctatggcggc gctggcacga atatctatcg ctgcaaggac gggcggtatg
      961 tccactttac gacgaacatg cccacatgtg gaaggagttt gcgcagaact ggatgaccga
     1021 caagtcgctc gccggttccg aatgggaaaa tccgcgttat cgtgacgagc actctgagga
     1081 agtcgccaag gccttcgccg gatttattgg tgagttcgag gcggacgatt ttgccaatca
     1141 ggcgcaagcg cgccatctcg ctgccgcgcc gcttaacacc gtcggccaat ttgtcgagtg
     1201 tgagcagaat cgcgcgcgcg agtggttgca ggaaatcaac cacccggtga ttgggaagta
     1261 caaggcgccc ggcgcaccca tgcggctgtc ggttacgccg atgcgggtgc ggcggccggc
     1321 gccgctgctc gatcaacacc gaaaagagat cctcgcggac ctcgataagt tgccggcgcc
     1381 ggcaatccaa cagcgcaaat ccgaatcggc gcgtcaaccg atgctcgccg ggctgcgcat
     1441 ggccgatctg acgcagcaat acgccggccc gcttggaaca gaactgctgg cgtattacgg
     1501 aatggaagtg gtgaagatcg agaccggcac gattcccagc aaagagcggc aagccgcagt
     1561 gcatgcgtgc atgaatcggg caaagctcgg ctgcacgctg aatttgcgca atccggaagg
     1621 aaaagaactg ttcaagcaac tcgtctcaag gtcggatgta gtcgttgaca acttcagctc
     1681 gggagttttg gagcgactcg gttttagttt taaggttctg cagaagataa atccaggcgt
     1741 cgtgcaggtc gtgatgcccg gctggggttt gaccgggccg ctgcaatcgt gggtcgcgtg
     1801 gggttggcag ctgctcgctt acacggggat catgcggttg tggggttacc cggactcgcc
     1861 gatggagact cgctgcaaaa ttgcctggcc tgatcgcgtc ggctcggtaa ccatggcgct
     1921 cggcgtgctt gcggcgctcg agtatcaagc acagactgga cgaggccagt ttatcgaagc
     1981 tggcatgctc gaggcgcaag gatcgatgat gggaccggcg attcttgatt ataccgtcaa
     2041 cggcaacgac tgggacgcga tgggttatcg cgagattctc ggcgagccgt acacaccgta
     2101 tggttgttat ccgtgccgcg gcgaagacgc gtggatcatc attgcttgcg ctggtgatga
     2161 acagtggcaa agcatggtta aattgatcgg cgcgaattcc tgggcagccg atgaaaaata
     2221 cgccagcgcg gcgggacgaa agcaattccg caacgagctt gatcagaagc tttcagaatg
     2281 gacatccagc tacactgttc gacaagtttt ccggatgctg caggagtcgg gcgttgcggc
     2341 cggcattcca tcatcgggag aagatctgtt tcacgacttt catctgcgcg cccgcggcca
     2401 catcgtggaa accgatgcgc agccatgggg taggatcgct catcacgggc tgccgggaat
     2461 cccatcgctg tcgagcgccg acgccgcgcg cggagcgcca tggatcggcg cacacaatgc
     2521 taaggtattt ggtgagattt taggactgag tagagagcga attgaggaat taaaaaaagc
     2581 ggaagcgatc aagtagcgtt ctagacgggg aaggattcct cacatccggt cggaatgacg
     2641 gcatgtgcgt attaccgtag cagtttcaaa cggtaggctg tcatttcgac cccttcgata
     2701 gaactcgggg taaactccgg gagaaatctt tccgaatcct acaccgggcg g
//
LOCUS       DASULZ010000313         2518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_241862, whole genome shotgun sequence.
ACCESSION   DASULZ010000313 DASULZ010000000
VERSION     DASULZ010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241862"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VFY96_06515"
     CDS             complement(<1..505)
                     /locus_tag="VFY96_06515"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606147.1"
                     /translation="MATYVRKSAYRRDPITAMLLFVVMLLFGSACREAFAGSAAEPTR
                     LRISIPGLGSSSYPLIMAQKKGYFRAEGYEVELIPMAAGLAVKTLLAGEVGVTAAGTV
                     LANLQGAKVRLVMGFIRELPYDLVVPPEIRRVEDLRGKKIAVADRGSVTFFVVRSILQ
                     AHGMEPDR"
     gene            646..1782
                     /locus_tag="VFY96_06520"
     CDS             646..1782
                     /locus_tag="VFY96_06520"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5606148.1"
                     /translation="MKNGFKIIDSDMHIMEPLDLWEKYIDPRFKERAPRPVRVPTRGD
                     LNLLMVDGKEPRQCSPTLAHDVRMISGRRNNTRGDVIDFARKQNFNAKSQLEAMDIEG
                     IDVAVLFPTACLHIMTMPDMDPLLAEAICRAYNDWLHDFCQENPKRLFGAALLPPHDV
                     SLAVREARRAREKLGFVTAFLRATPLPGQTWFSVYWEPLWAELEEIGMTVGFHECTNS
                     GHYPHLERFGTGNRLLRHICTHVTGQMVTMVDIIVGGVLERFPQLKVAFLECNCGWAP
                     SWLQRMDRHWQQLGKHDAPLLTIKPSEYFKRQCVIGCEGDEADIGNVCQLIGDDNLVF
                     SSDYPHSDSDFPKATQEFFHQEMPADSRRKILWDNCVKFYGIEG"
     gene            complement(1846..>2518)
                     /locus_tag="VFY96_06525"
     CDS             complement(1846..>2518)
                     /locus_tag="VFY96_06525"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606149.1"
                     /translation="VVMLAGLNGLDQILVALPIIKRGADLLGKRVGISRFGTTADYGA
                     RIGLKKLKLQAQKDVTLIQIGDTPARIGGMLSGAIEAATLNSSEKELAFRNGFHILAD
                     PSDVEFPGNAIVTTKSFMKTNREDVKRFVRALVEVIHFAKTQPERGKKLLQEIYRQND
                     AAVIAKRYDAMVEMFPDYPYLTRGSVLSFLEILRDEGKLKDPLYPDPFLDTSLLSEVE
                     RERKK"
BASE COUNT          565 a          753 c          659 g          541 t
ORIGIN      
        1 cccgatccgg ctccatgcca tgcgcctgca aaatcgaccg cacgacgaag aaagtcacgc
       61 tgccgcggtc ggccaccgca atttttttgc cgcgcagatc ttcgacccgt ctgatctccg
      121 gcggcaccac gagatcgtac ggaagttcgc gaataaaacc catcaccaag cgcaccttcg
      181 cgccttgcaa gttcgccaac acagtgcccg cggcagtgac accgacttcg ccggccagta
      241 acgtctttac cgcaagcccg gctgccatgg gtatcagctc gacttcatag ccttcagcgc
      301 ggaaatagcc tttcttctgc gccattatca aagggtaaga tgatgaaccg aggccgggaa
      361 tgctgatgcg caagcgcgtg ggctccgcgg cactcccggc aaaagcctct cggcatgcgc
      421 taccgaatag cagcatcacg acaaagagca acatcgctgt tattgggtca cgcctatatg
      481 ctgatttacg gacgtatgtt gccatcgtgc cgactcccta gtgactcgct gcgcgaatct
      541 gtattcctcc tggttcgctc atgtcaagct tacggttgac agcgcctccg cgccagttgt
      601 agttggagat aaccgaacgg cgtcattttg acgtggggaa gaccaatgaa gaatggattc
      661 aagatcatcg actccgacat gcatatcatg gaaccgctgg atctttggga aaaatacatc
      721 gacccgcgct tcaaagaacg cgcgccaagg cctgtgcgcg tgcctacgcg cggcgattta
      781 aatctgctca tggtcgacgg taaagaaccg cgccaatgta gtccgacctt ggcccacgac
      841 gtgcgcatga tcagcggcag gcgaaataac acccgcggcg atgtcatcga cttcgcgcgc
      901 aagcaaaatt tcaacgccaa gtcgcagctc gaagcgatgg atatcgaagg catcgacgtg
      961 gccgtgctct tccccaccgc ctgtctgcac atcatgacga tgccggacat ggacccgctg
     1021 ctcgccgaag cgatctgccg cgcttacaac gattggctcc acgacttctg ccaggaaaat
     1081 cccaagcgtc ttttcggcgc cgcgctgctt ccccctcacg atgtctcgct tgccgtgcgc
     1141 gaggcgcgcc gcgctagaga gaagctcgga ttcgtaaccg cgttcctgcg cgccaccccg
     1201 ttgccgggac agacttggtt cagcgtctat tgggaacctt tatgggcgga actagaagag
     1261 ataggcatga ccgtcggctt tcacgaatgc accaattcag gacactaccc gcatcttgaa
     1321 cggttcggca caggcaaccg cttgctgcgt cacatctgta cccacgtcac cgggcaaatg
     1381 gtgacaatgg tcgatataat cgtcggcggc gtattggaac gttttccgca gctgaaagtc
     1441 gcattcctcg aatgcaactg cggctgggcg ccttcatggc tgcagcgcat ggaccggcat
     1501 tggcaacagc tcggcaaaca tgacgcgccg ctgctgacga taaagcccag tgaatacttc
     1561 aaacgccagt gcgtgatcgg ctgcgaaggg gacgaagcag atatcggtaa cgtctgccaa
     1621 ttgatcggcg acgataatct ggttttttcc agcgactatc cgcacagcga ctcggacttc
     1681 cccaaagcca cccaggagtt ttttcatcaa gagatgccgg cggacagccg caggaagata
     1741 ttgtgggata actgcgtcaa gttctatggg atcgagggtt aacgcggttc aaagttcaag
     1801 ctgaagcgcc gatcatactc ttgattcctt gaactccgga accagctatt tttttcgctc
     1861 ccgctctact tccgacaaca agcttgtatc gagaaatggg tccgggtaaa gcggatcttt
     1921 cagtttccct tcgtcgcgca gtatttccag gaatgacagc accgaacccc tggtcaaata
     1981 gggatagtcg ggaaacatct ccaccatggc atcgtaacgc ttggcaatca ctgccgcgtc
     2041 attctggcgg tagatctctt ggagcagttt tttgccgcgc tcaggctgag ttttcgcaaa
     2101 atgaatcact tcgaccaaag cacgcacgaa tcttttcacg tcctccctgt tggttttcat
     2161 aaatgacttc gtggtcacga ttgcgttgcc gggaaattcg acatcgctcg gatcagccag
     2221 gatgtgaaag ccattcctga aggccagctc cttttcactg ctgttcaacg tcgccgcctc
     2281 gatggccccc gacaacatcc ctccgatgcg cgccggagtg tcgccgatct ggatcaacgt
     2341 cacatctttt tgcgcctgca acttcagctt cttgagcccg atgcgagcgc cataatccgc
     2401 ggtagtgccg aaacggctga tgccgacccg tttaccgagc agatcggcgc cgcgtttgat
     2461 gatcggcaga gcgaccagaa tctgatcgag accattcagc ccggcaagca tgaccaca
//
LOCUS       DASULZ010000314         4207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_952886, whole genome shotgun sequence.
ACCESSION   DASULZ010000314 DASULZ010000000
VERSION     DASULZ010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952886"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /gene="glmS"
                     /locus_tag="VFY96_06530"
     CDS             <1..1396
                     /gene="glmS"
                     /locus_tag="VFY96_06530"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX5606150.1"
                     /translation="RRTLKEIHGSYALLFLNRRDPKRLIVAKNSTPIVIGWGEGETFI
                     ASDIPALLDYTRRVTFLEDGEIGEVTLGSYRLFDGKGKTIQRPVKEITWDAVAAQKGG
                     YAHFMLKEIHDQPRALADTFRGRISLKDGQVSLEDIKLTATAVKQIKRFHLVACGTAW
                     HACLVGKFLLEEIAGIPAEVDYGSEFRYRSPLMDPKSVLLMVSQSGETADTLAATEIA
                     RAKRAKILSVCNVIDSSIARKSDGVLYTHAGPEISVASTKAFTTQLTALYLLAVALGR
                     LTGRLKTHEARKLLRDLMSLPSWIEKSLELENEVKDLARDLMHSRDFLYLGRGINYPI
                     ALEGALKLKEISYIHAEGYPAGEMKHGPIALIDEKMPVIMLAPRDRYFQKTVSNLKEV
                     ESRGGKVVVLTDDSKTATEIAAHRTLTLPKASHYLTPIVMTVPLQLLAYYIAVHRGTD
                     VDQPRNLAKSVTVE"
     gene            1653..3137
                     /locus_tag="VFY96_06535"
     CDS             1653..3137
                     /locus_tag="VFY96_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5606151.1"
                     /translation="MAGGLLAAAFLRAADVGAFTSAGAFRWAGDPEGGAPFVEADPLH
                     PDRLVGFDVEIAELIARKINRIPEFVNITFTSIDQSIERGDAEIGLSGIEDTPARRAT
                     MAPTIPYYEFREVLSVRHADQTQFRTLADLKGRRVGTLGGTIAYQILLNAQREFGIHP
                     VSYDDDVHPYSDLVIGRIDAVLLDNVLAERRTRNLSGLAIQPQSVAMGHYVGILAARN
                     SALRDQIDDILRTAMRDGTLERIFRKWRVWNDDQPRLYAQLLAAQPVAGVTGFEASPV
                     VLTAWQAILRYLPSLLRASLVTIVLSCLAMLLAIVLGISIATGRVYGTRLMRSLLTGY
                     VELMRGTPILLQLFVLYYGIADLIRLPAFGAALLGLGLNYAAYESEIYRGALQAVPVG
                     QLEAARILGLNEARVLTLVRGPLALRFALAPMTNDFIALLKDSSLVSVLTVFELTKQT
                     QIFATNIGSWVIPGLLCAALYLTMSLPLATLARRLEQRWRFSTA"
     gene            3134..3823
                     /locus_tag="VFY96_06540"
     CDS             3134..3823
                     /locus_tag="VFY96_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5606152.1"
                     /translation="MSHCVLRLQEVTLLRSGRQVLAGVSFGVHRGELVALMGPSGVGK
                     TTVLRVIAGLETFQSGTVHVEGIAINGANGHPPRQLRDLRAKVGMVFQFHHLFEHLSV
                     LKNVWLAPVYARGIPPREAENRARALLAALGVEHRAHAFPRELSGGEAQRVAIARALA
                     VDPPLLLLDEPTASLDANRRAELRDLLCGLTGEGRTLVVATHDEPFARDCASRVLRVR
                     EGSVFDDAAMA"
     gene            4024..>4207
                     /locus_tag="VFY96_06545"
     CDS             4024..>4207
                     /locus_tag="VFY96_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606153.1"
                     /translation="MKMSGMALMKCAELQKQFGCGTIRQLKITSLTKPDNVPFFWPWL
                     RGRRECPERANDQQVRE"
BASE COUNT          823 a         1206 c         1197 g          981 t
ORIGIN      
        1 ccggcgcacg cttaaggaga ttcacggctc ctacgcgctg ctgtttctga atcgtcggga
       61 tcccaaacga ttgatcgtgg caaagaattc cacgccgatt gtcatcggct ggggcgaagg
      121 cgaaactttc atcgcctcag acatcccggc attgctcgac tacacgcgcc gcgtgacatt
      181 tctcgaagac ggtgaaattg gcgaagtgac gctcggctct tatcgccttt tcgacggcaa
      241 gggaaaaact attcagcggc ccgttaaaga aatcacctgg gatgcggtgg cggcacaaaa
      301 gggcggctat gcgcatttca tgttgaaaga aattcatgac caaccgcgcg cgctggcaga
      361 tacgtttcgt ggccgcatct cattaaaaga cgggcaagta tcgctcgagg acatcaagtt
      421 gactgcgacc gctgttaaac aaatcaaacg ctttcacctg gtcgcatgtg gcacggcatg
      481 gcatgcctgc ctcgtgggca agtttttgct cgaagaaatc gccggcatcc cggcggaagt
      541 cgactatggt tcggagttcc gctaccgttc gccgctgatg gatccaaaat ccgttttgtt
      601 gatggtcagt caatcgggtg aaaccgccga caccttggcg gcgaccgaga tcgcgcgcgc
      661 gaaaagggcg aaaatcttgt ccgtctgtaa tgtgatcgat tcttcgattg cgcgaaagtc
      721 tgatggtgtg ctgtatactc acgccgggcc cgagatcagc gtggccagca ccaaagcgtt
      781 tacgacccaa ctgaccgcgc tttaccttct ggcggttgcg ttgggacggc tcaccggcag
      841 actcaagacc catgaggcgc gcaagctttt gcgtgatctc atgtcgttgc cgagttggat
      901 cgagaagtcg ttggagctcg aaaacgaggt caaagattta gcgcgcgatc tcatgcacag
      961 cagggatttt ctctacctcg gtcgcggcat caattacccc atcgccctgg aaggcgcgct
     1021 caagctcaag gaaatttcct atattcacgc agaaggttat cctgctggcg aaatgaagca
     1081 cggcccgatt gcgctgattg atgaaaagat gccggtgatc atgctcgcgc cacgggatcg
     1141 ctattttcag aagaccgtga gcaatctgaa ggaggtcgaa tcccgcggcg gcaaggtcgt
     1201 ggtgttgacg gatgattcca aaactgcgac cgagatcgcc gcccatcgca cgttgacgct
     1261 gcccaaggct tcgcattatc ttaccccgat cgtgatgact gttccgctcc aactgcttgc
     1321 ttattatatt gcggtacatc gcggcaccga cgtcgatcag cctcgcaatc tcgccaaaag
     1381 tgtgaccgtc gaatgacagc gcgtcgaagc gaggctccct cgcgctcgcg cgcacgttcc
     1441 attcgctccg gcaccgcgat tttcggctgc tttttttcgg cacccgcctt tctcacatcg
     1501 gtattttatc caggcgccga ttgacatcca gatctgccat agctagattg cgcgcaatcg
     1561 atggccattc cattgttttt taaattggcc gaagctgaat tgttcactgc attcatctcc
     1621 cgatgaacgt tatgcgcagg aggctgcgta ctttggccgg tggcctgctt gccgcggctt
     1681 ttttgcgggc ggccgatgtc ggcgcgttca cgtcagccgg cgcattccgt tgggcgggcg
     1741 atccggaggg cggagcgccc ttcgtcgaag ctgatcccct ccacccggat cgactcgtcg
     1801 gtttcgatgt cgaaattgcc gagctcattg cgcgaaagat caatcgcata cccgagttcg
     1861 tcaacatcac cttcacctcg atcgatcagt cgatcgagcg aggcgatgcg gagattggcc
     1921 tcagcggcat tgaagatacg cccgcgcgcc gcgccaccat ggcgccgacc atcccctact
     1981 atgagtttcg cgaggtgttg agcgtccgcc atgctgatca aacgcagttt cgcaccctcg
     2041 ccgatctcaa agggaggcgc gtcggcactc tcggcggcac gattgcatat cagatcttac
     2101 tgaacgctca gcgtgagttt ggcatacacc ccgtttctta cgatgacgac gttcacccat
     2161 acagtgatct cgtgatcggc cgcattgatg ccgtcctctt ggacaatgtt ttagcggagc
     2221 ggcgcacccg taatttgtcg ggtttagcca ttcagccgca gagcgtcgcc atgggtcatt
     2281 acgtcggaat tttggcggca cgcaacagcg cgctccgcga tcagatcgat gacattctgc
     2341 gcaccgccat gcgtgacggc accttggaaa gaatttttcg caaatggcgt gtttggaatg
     2401 atgaccagcc gcgactttac gcgcaactgc tcgcggcgca accagtggcc ggcgtaaccg
     2461 gctttgaagc gtcccctgta gtgctcacgg cgtggcaagc gattctacgt tatttaccat
     2521 cgcttctgcg cgcctcactg gtgacgattg tgctttcctg cttagcgatg cttttggcaa
     2581 ttgtccttgg aatttcaatc gccaccggcc gcgtctacgg tactcgcctc atgcgctcgc
     2641 ttttaacagg ctatgtcgag ctcatgcgcg ggacgccgat cctgctgcag ctgttcgttc
     2701 tctattatgg catcgctgat ctgatccgcc tgccggcctt tggcgccgcg cttctggggc
     2761 tcgggctgaa ctatgccgct tacgagagcg aaatttatcg cggcgctttg caagccgtgc
     2821 ccgtgggcca acttgaggcg gcgcgtattc tgggcttgaa cgaggcccgg gtactgacgc
     2881 tcgtgcgcgg cccattggcg ttacgcttcg cgttggcgcc catgaccaac gacttcattg
     2941 cattgctgaa agattcttca ttggtctcgg tgctcacggt tttcgaactc accaaacaga
     3001 cacagatttt tgccacaaac attggtagct gggtcatacc ggggctgctc tgcgccgccc
     3061 tctatctcac catgtcgcta ccgctcgcca cactggcgcg tcggctcgag cagcgctgga
     3121 ggttttcaac ggcatgagtc actgcgtgct gcgcttgcaa gaagtcacgc tgcttcgcag
     3181 tggccgtcaa gtcctcgctg gggtcagctt cggcgttcac cgtggagaat tggttgcgtt
     3241 gatgggtccg tcaggcgtgg gtaaaacgac tgtgcttcgt gtgattgccg ggcttgagac
     3301 gtttcagtca ggaaccgtgc acgtcgaagg aatcgcgata aacggcgcta acggacatcc
     3361 gcccagacag cttcgcgacc ttcgcgccaa agtcggcatg gtctttcaat tccatcatct
     3421 ctttgaacac ctttcggtgc tcaagaacgt ctggctcgct ccggtttacg cacggggaat
     3481 tcccccacga gaagcggaaa accgcgctcg cgcgctactt gcggcactcg gggttgaaca
     3541 tcgggcgcac gcgttcccgc gcgaactctc gggcggcgag gcgcagcgcg ttgcgatcgc
     3601 ccgcgcccta gcggtggatc cgccgctgtt attgctggac gagccgaccg cgtccctcga
     3661 tgccaatcgt cgcgcggagc ttcgcgatct tctttgcggg ttgaccggcg aaggtcgtac
     3721 tctcgtggtt gccacccatg acgaaccgtt tgcgcgtgac tgcgcatctc gagtgctgcg
     3781 cgttcgagag ggttctgtgt tcgacgacgc cgcgatggcc tgatttgagc ttgttcactc
     3841 tgtgccgcag cgccccaata aatttagccg attttttatt tttgtcttgt catcgccaag
     3901 attttgcgga tactgagata gatgtcgaac gtggttcgaa gcgcccgata caataagcgc
     3961 gcaattgata gtcggatagc gctggatcag tggggttatc cgagtggaat ggtgtggtgt
     4021 ccgatgaaga tgagcggaat ggcgctgatg aaatgcgctg aactacaaaa acagttcggc
     4081 tgcggaacta tccgccagct caagatcacg tctttgacca aaccggataa cgtgccgttt
     4141 ttctggccct ggctccgggg acgccgggaa tgcccggaac gagccaacga tcaacaggtg
     4201 cgtgaat
//
LOCUS       DASULZ010000315         5852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_749443, whole genome shotgun sequence.
ACCESSION   DASULZ010000315 DASULZ010000000
VERSION     DASULZ010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749443"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VFY96_06550"
     CDS             complement(<1..615)
                     /locus_tag="VFY96_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606154.1"
                     /translation="MDLSSYIVNARPGVLGGKHEEKRPLRQLSSLPVNRYVFINRDSH
                     PEANIYVAIHQAKNLPSPVPDYQVPHCHNTDEFYYFIGENPDLTGLEGQIIFEGKIHK
                     IISPACIYIPTGTVHEYKVTKGAGTVTVLFRDCGYTHEDKPFDNAKGERDFARYAKYI
                     FSPDVRPTTEIKYHTDAAPGVRYVFVDGKLQPDAGFYTVVRSVAN"
     gene            complement(652..1680)
                     /locus_tag="VFY96_06555"
     CDS             complement(652..1680)
                     /locus_tag="VFY96_06555"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5606155.1"
                     /translation="MNLFLLLVFFLTLHASTAAAQSDFFKGKQIRIIVGASAGAASDL
                     YARVVANYLPKQIPGRPEIIVQNMPGGGSITAANYVYSVAKPDGLTLGAVTSPIYFAQ
                     LLGRKEVQFDWEKFTWIGTPEENDELFFIKSDLPYKTLLDLRKATEPPRCGASGVGST
                     GYYIPKLLEEIFGFRLALVTGYPGAADVDLAVEKNEVHCRGTTISAFFGREPGKTWAK
                     NGYVRFLVQTGDKRNHRLPETPTIWELMDEAKASEANRRLARVVLGPGAFGRPILASP
                     GIPAQRVKLLRDGYDKMLKDADFLAEANKRQWEISPVSGERLATLAKEVIHQPPDIIE
                     RMKKVLGE"
     gene            complement(1681..2601)
                     /locus_tag="VFY96_06560"
     CDS             complement(1681..2601)
                     /locus_tag="VFY96_06560"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEX5606156.1"
                     /translation="MSPGRKLKVLLRRSGKVLAVLGAPNAFHAKIMEANGVEACFVGT
                     SITGGNYTGLPDVGILSSTECVGIARWIAHSVEIPVILDGDTGHGGIMAVRRLVRECV
                     EAGLAGLRLDDQPLEQKRRTMSEGISVIARDEAVIRYRSAVDAKNEIDPNFVIMAQCY
                     ARNAVNGGMAELLKRLPLYEKAARVDWVQFEAPHSVAEIKRARAVVKGPFSAMKGQLP
                     RALTLAEHKALGLNAAWYTFLPDQVLKISAWTFFQKFKKNDVMAWQEFMQAHQGTPFT
                     GGEELPWDVTGMGELAKFEAKYAVKQKRRS"
     gene            complement(2605..3750)
                     /locus_tag="VFY96_06565"
     CDS             complement(2605..3750)
                     /locus_tag="VFY96_06565"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5606157.1"
                     /translation="MARNGYRIFDADTHVRPDADLLEPYLPAAKRAPLLNYESYKVRN
                     KEGAVTYLMGERKYKRRLGLAAEAAPDRSEYMSGYKRHQHGNPNPLSEREPAARIRDM
                     DIEGIDVNLMLPSGWFGCWTTINDVGLETAIYEAYHRWMSDYCAAFPNRLKGVILAAA
                     RDVKTSVAEIERAAKETWPLAVFVYAPHQFPLDHPDLEPIWKAAADHDLAIALHTFTV
                     MPPYAPGGLDSWDNLWLQRSAAHPWCGQRNMASIIGAGIMDRYPKIRVGTLEAGHGWL
                     PAWVNRLEEHTKLCPEALPHLQQTIRDYVTSGRYFQSIEVSEGGAITQSVLDLLGTDI
                     LMFGSDYPHGESWFPVSVETVLGWKLKEADMKKLLWDNAVRYYGRYS"
     gene            3902..5374
                     /locus_tag="VFY96_06570"
     CDS             3902..5374
                     /locus_tag="VFY96_06570"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018461143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5606158.1"
                     /translation="MAKMLVASAQVDSDSGQTTEVRNPATGEIVDTVPKGTVNDVRRA
                     IDVAQSALKKWSTMAPAKRGAILLAAGHTILQQEKELATLLTKEQGKPMRESILEIRR
                     FVHTLDHYGGMAKTMRTSAVMLDTGRHGLVLRKPLGVCGSIVPWNFPVSLMGNKLGPA
                     LLAGNTVVVKPAGTTPLTDIRCCEIIDRAIQEAGGPKGVLNVVTGPGSVVGEELLVSP
                     KVRKIGFTGATDTGRRVMQSASKDFKHVTLELGGSDPMIVCDDADLDRAVSAASVGRF
                     FNCGQACLAVKRLYLFDSIADPFMEKLVEKAKKISVGNGLKAGVLMGPLHTKEQRQEV
                     EEQVEDAVKRGAKVLYGATRPKGDEYDKGFYLQPTLVADVDPASRMIQEEVFGPALPI
                     IRVKNLEQAIEQANGSIFGLGSSIWTRDINRAIYGAENIEAGYTWVNSAQIIYDELPF
                     GGVKQSGLGKEHGDEAIEHYTDSKSVVIATDVQGEIIGGE"
     gene            5420..>5852
                     /locus_tag="VFY96_06575"
     CDS             5420..>5852
                     /locus_tag="VFY96_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5606159.1"
                     /translation="MKVDLPDVYNAATTFVEENIAQGRGGKIAVYYEDQKITYQEVFE
                     KVNRTGNALRDLGIEIENRVLLILPDSPAFAYSFFGAIKIGAVAVPTNPWMFAKDYEY
                     LINDSRARAIIVHETVLPEIEKIWNKTPFLKRVIVVGAARGK"
BASE COUNT         1325 a         1633 c         1593 g         1301 t
ORIGIN      
        1 gttcgcgaca ctgcgcacaa ccgtgtaaaa gccggcgtcg ggctgaagct taccgtcaac
       61 aaagacgtag cggactccgg gggccgcgtc ggtatgatac ttgatttcgg tcgtcggacg
      121 cacgtccgga gagaaaatgt acttggcata tctggcgaaa tcgcgctcgc ctttggcatt
      181 gtcgaaaggc ttatcctcat gggtatagcc gcaatcgcga aacagcacgg tcaccgttcc
      241 ggctcctttg gtaactttat actcgtgaac cgtaccagtc ggaatataaa tgcacgccgg
      301 agagattatc ttatgaatct ttccctcgaa aataatttgg ccctcgagcc cggtcagatc
      361 cgggttttcg ccgataaaat aatagaattc gtcggtgttg tggcagtgcg gcacttgata
      421 gtcgggcacg ggcgacggga gatttttcgc ttggtgaatc gccacataaa tattcgcttc
      481 aggatgagaa tcgcgattga taaagacgta gcggtttacc ggcaacgagc tcaattgccg
      541 cagcggcctt ttttcttcat gttttccgcc gagcaccccg ggccgggcat tgacgatata
      601 gctgctcaga tccattgcgc ccccttcgta aagtctgatg cgtgcggttg ttcactcacc
      661 caaaactttt ttcatccgct cgataatatc cggtggttga tgaatgactt ccttcgccag
      721 agttgcgagt ctttcgccgc ttacagggct aatttcccat tgacgcttgt tcgcctcggc
      781 gagaaagtcg gcgtccttca gcattttgtc atagccatcg cgaagcagtt tcactctttg
      841 cgcaggaata cctgggctag ctaagatggg tcggccgaag gcgcccggcc cgagaaccac
      901 acgcgccaga cgcctgttcg cctcggaagc ttttgcctcg tccatcaatt cccagatcgt
      961 tggtgtttcc ggcaggcgat gatttcgttt gtcgccggtt tggaccaaaa agcgcacgta
     1021 accattcttt gcccatgtct tccctggttc ccgaccaaaa aacgcgctga tcgtcgtgcc
     1081 acggcagtga acctcatttt tttccactgc caagtcaaca tcggcggcgc ccggatagcc
     1141 agtgaccaag gcaagtctaa aaccgaaaat ctcctccagc agcttgggaa tataatagcc
     1201 cgtgctgccg acgcccgatg cgccgcaacg cggcggctcg gttgctttgc gcaaatcgag
     1261 cagagtttta tagggcaggt cggacttgat gaaaaacagc tcgtcgtttt cctcgggagt
     1321 gccgatccag gtaaatttct cccagtcgaa ctgcacttcc ttgcgaccca gcagttgggc
     1381 gaagtagatg ggcgaggtaa cggcaccgag ggtgagtccg tcaggtttgg ccacgctgta
     1441 cacgtagttc gccgctgtga tggagccgcc acccggcata ttttgaacaa tgatttccgg
     1501 tcttcctgga atctgtttcg gcagatagtt cgcgaccacg cgcgcataca gatcactggc
     1561 cgcgccggcg gaagcaccga cgataattct tatttgcttg cccttgaaaa aatcactctg
     1621 tgctgcggcc gtggaggcgt gaagcgtcaa aaaaaacact agcaaaagaa acagattcat
     1681 ctacgatctc cgcttctgct tgactgcata tttcgcttca aacttggcaa gttcccccat
     1741 cccagtgaca tcccacggca actcttcgcc gccagtaaag ggcgttccct gatgtgcttg
     1801 catgaactct tgccaagcca tgacatcgtt cttcttaaat ttctgaaaaa acgtccaggc
     1861 ggaaattttc agcacctgat cgggaagaaa agtgtaccag gcagcattga gacccagcgc
     1921 cttgtgttcg gccaatgtga gcgcacgagg cagctgacct ttcatcgccg aaaagggacc
     1981 cttcaccacg gcccgcgcgc gcttaatttc cgccacggag tgcggcgctt cgaattgcac
     2041 ccaatcgacg cgcgccgctt tttcatagag cggcaatcgt ttcagcagtt ccgccatccc
     2101 gccgttcacc gcattgcggg catagcactg tgccataatc acgaagttgg ggtcgatctc
     2161 attttttgca tcgactgccg agcggtaacg gattaccgct tcatcgcgcg ctatgaccga
     2221 aataccctcg gacatcgtgc gccgtttttg ctccaagggt tgatcgtcga ggcgcaaacc
     2281 cgcaagtccg gcctcgacgc attctcgaac cagccggcgt accgccatga tgccgccgtg
     2341 accggtgtcg ccgtcgagaa tgaccggaat ctcaacgctg tgggcaatcc atcgtgcgat
     2401 gccgacgcat tccgtcgagg ataggatgcc gacatcagga agtccggtat agtttccacc
     2461 ggtaatgctc gtgccgacga aacaagcctc gacgccgttg gcttccataa ttttcgcgtg
     2521 aaaggcattg ggcgcaccga gtaccgcgag aacttttccg gatcggcgga ggagcacttt
     2581 taactttcga ccgggactca tcgattagga gtaacgtccg taatagcgga ccgcgttgtc
     2641 ccataggagt tttttcatat cggcttcttt taacttccag ccaagcacag tctcgacgga
     2701 cacgggaaac caactctcgc catggggata atccgacccg aacatgagaa tgtcggtgcc
     2761 gagcaggtca agcacactct gggtgatcgc gccaccttcg gatacttcga tactttggaa
     2821 gtagcgtccg ctggtgacat aatcccggat cgtttgttgc aaatgcggca acgcctccgg
     2881 gcagagtttt gtgtgttcct ccaatcgatt cacccacgcc gggagccagc catggccggc
     2941 ctcgagcgtg ccgactcgga tcttgggata gcggtccatg atcccggcgc cgatgatcga
     3001 cgccatattg cgctgaccgc accatggatg cgccgccgac cgctgcaacc acaaattgtc
     3061 ccaggaatcc aacccgccgg gcgcgtaggg cggcatcacg gtaaacgtat gcagagcgat
     3121 cgccaagtcg tggtcggctg cggctttcca gatcggttcc aagtctggat gatcgagcgg
     3181 aaattgatga ggtgcgtaaa cgaaaaccgc caagggccag gtttccttgg ccgcccgctc
     3241 gatctcggca actgaggtct tcacatcgcg tgccgcagca aggatcaccc ccttcaaccg
     3301 attcggaaag gccgcgcagt aatcggacat ccaccggtgg taagcctcat agatcgcggt
     3361 ttccaaaccg acgtcgttta tagttgtcca gcaaccgaac caaccggagg gtagcatcag
     3421 attgacgtcg atcccttcga tgtccatgtc acgaatccgg gcggcgggct cccgctcgga
     3481 caacggattg gggttgccgt gctgatgacg tttgtagccg ctcatatatt cgctacgatc
     3541 gggcgcggct tcagcggcaa ggcccaaccg ccgcttatat tttcgttcgc ccatcaggta
     3601 cgtcactgcg ccctctttgt tgcggacctt ataactttca taatttaaca atggtgctcg
     3661 tttcgcggcc ggcagatacg gttccaacag atccgcatcg ggtctgacat gagtgtcagc
     3721 atcgaaaatt cgatatccat tgcgtgccat tgcgacctct tgctgctctc aattagctca
     3781 ccaacgatgg actagcgata aacgtggtag cttgtcaatc tgtgcgcagt acgaattctt
     3841 gacttccacc tatgaacatg atttttctct attttctgac agagcaatca ggaggatcat
     3901 catggcaaaa atgttagtcg ccagcgcgca ggtcgattcc gacagcggac aaaccaccga
     3961 ggtccgcaat ccggccactg gcgagattgt cgacaccgtc cccaaaggaa ccgtcaacga
     4021 tgtccggcga gcgatcgatg tcgcgcaatc cgcactaaaa aagtggtcca ccatggcacc
     4081 ggccaaacgc ggcgcgattt tgctcgctgc cggccacacg attcttcagc aggaaaaaga
     4141 acttgccaca ttgctgacca aagaacaggg taaaccgatg cgcgaatcga ttttggaaat
     4201 ccgccgattc gtccatacct tggatcatta tggcggcatg gcaaagacca tgcgcaccag
     4261 cgccgtaatg ctcgacaccg gtcgtcacgg gctggttctc cgtaagccgc tgggtgtatg
     4321 cggatcgatc gtgccatgga actttccggt ctccctcatg ggaaacaagc tcggtccggc
     4381 attgctggcg ggcaacacgg tcgtcgtcaa gcctgccggc accaccccgc tgaccgatat
     4441 tcgctgctgc gagatcatcg atagagcgat tcaagaagcc ggtggcccca aaggcgtctt
     4501 gaacgttgtg accggtccgg gaagcgtagt cggtgaagag cttttggtca gtccgaaggt
     4561 ccgcaagatc ggcttcacgg gcgcgacgga cactggccgc cgagtgatgc agtccgcgtc
     4621 taaagatttc aagcatgtga ccttggagtt gggcggcagc gatccaatga tcgtatgtga
     4681 tgatgccgat ctcgatcggg cggtgagcgc cgcctccgtc ggccggtttt tcaattgcgg
     4741 gcaggcctgc ttagcggtga aacggctgta tctcttcgac agtatcgccg atccgtttat
     4801 ggagaagctg gtcgaaaaag cgaaaaagat cagcgtcggt aacggtctca aagcgggagt
     4861 actaatgggc ccgcttcaca ccaaagagca acggcaagaa gtcgaagagc aggtggaaga
     4921 cgcggtgaaa cgcggcgcaa aagttcttta tggagccacc agacccaaag gcgatgagta
     4981 cgacaagggt ttttaccttc agccgacttt agtcgcggat gttgatccgg cttcgcgaat
     5041 gatccaagaa gaagtctttg gtccggcgct gccgatcata agggtaaaaa atctcgagca
     5101 ggcgatcgag caggctaatg gctcgatctt tggtctcggc tcatcgatct ggacacgcga
     5161 tatcaaccgg gcgatatatg gcgcggaaaa catcgaagcg ggttacacct gggtgaattc
     5221 agcgcagatc atttacgacg agttgccgtt cgggggcgtg aaacaaagcg gccttggcaa
     5281 agagcacggc gatgaagcga tcgagcatta tacagattcg aagtcggtcg tcatcgccac
     5341 cgacgtccaa ggcgagatta tcggaggaga ataattagcc gctcaaggct tccgacccgg
     5401 agcgttcagt ccgccgttca tgaaagtcga tctccccgac gtttataatg ccgccacgac
     5461 ctttgttgaa gaaaacattg cacaaggtcg cggcggcaaa atcgctgtct actacgaaga
     5521 ccaaaagatc acctaccagg aagttttcga aaaggttaac cgcaccggca atgcgctaag
     5581 agatctcggt atcgagattg aaaaccgcgt gctcttgatc ttgccggatt cgccggcgtt
     5641 cgcctatagc tttttcggcg cgatcaagat cggcgccgtg gcagtgccga ccaatccttg
     5701 gatgttcgcc aaggattacg agtatctcat caacgatagc cgcgcgcgtg cgatcatcgt
     5761 acacgagacg gttttgccgg aaatcgaaaa gatctggaac aagaccccgt ttttgaagcg
     5821 cgtcatagtt gtcggcgcgg cgcgcggcaa gg
//
LOCUS       DASULZ010000316         3624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_141293, whole genome shotgun sequence.
ACCESSION   DASULZ010000316 DASULZ010000000
VERSION     DASULZ010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141293"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            462..1184
                     /locus_tag="VFY96_06580"
     CDS             462..1184
                     /locus_tag="VFY96_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX5606160.1"
                     /translation="MRYELFYQPSIQGRGEFVRLPLEDAGAEYIDVARDPNFGRPGIM
                     KFLEDPSLEHPPFAPPFLKAGKLLISQTANILQFLGPRLDLVPKSEASRIWANQLQLT
                     IADWLYEAGQTHHPIANVLYYEEQKEEAKKRAGHFIANRIPKFLGYFERILKQNANGG
                     DFVFGKRVCYVDLSLFQMIEGLRYAFPKAMARLEPQHPQMVSLHDRIKARPRIAAYLS
                     SPRRLAFNEQGIFRHYLELEES"
     gene            complement(1492..>3624)
                     /locus_tag="VFY96_06585"
     CDS             complement(1492..>3624)
                     /locus_tag="VFY96_06585"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX5606161.1"
                     /translation="AAYVPLEPFRLDRAEQPCVIALPVPEPYGYRRISNGAIEKSLPD
                     AVGAFIQWLINESGWQVAERPALAKASLAPSAALGFRPGETEHLVPIQARHVCVLFRR
                     FISYQEDMTRPYVEALEARGIPHLLVGGRSFHNRAEIEALRAALAAIEWPEDELSVFA
                     TLRGSLFAIGDEELLEYRHKFGRFHPFRIPEKAPENLAAIVRALQKLQALHRSRNRVP
                     IATTIAELLQSTRAHVGFALEHGGEQVLANVAHVSDLARRYEAEGGISFRGFIDELRQ
                     QAESGEVGEAPILEEGSDGVRLMTVHRAKGLEFPVVVLADITAKLRGSVASRTIDSDR
                     GICAIRLAGCSPAELLEHEQEELRRDEAEGARVAYVAATRARDLLVIPAVGDEERDGW
                     IRPLNSAIYPPIGKRRQQVGPPGAPEFRSSDSVLVRPGGTAAGPNTVAPGLHMLKPDA
                     LLTPYPRSAGEDAGVGDFSVVWWDPRVLKLGAEAPLGIRRPDLIVKDVAPQTVEADLS
                     AYKGWRNKRDTAIATGAQNSMAVRTVIESAKIAGTAGQTPTVSAVEVVELDRATNRPS
                     GPRFGTLVHAVLASVPLDGDLTLIRELATVHGRIIGATAEEIAAAGEAVSTALAHSVF
                     DRARSAAKQNRCRREAPITWRDPDGFLTEGVVDLAFEQDQRWIVIDFKTDEDLHEHEI
                     VYRRQVGLYAAAIEAATGRPVTAMLMRV"
BASE COUNT          784 a         1060 c         1017 g          763 t
ORIGIN      
        1 gacatggccg ctgacatcga tacaggtaaa tccttcgagc gctccggaaa tcatggttca
       61 atttacttcg cttcgtgctc tctatgtcac tcccggggaa gactggaatt caagtttcgg
      121 agggcgcgat tccaagcgcg ctatccgcac ggccttccgt gctttatcga atgacgacag
      181 atataagttg ccttatcgca atcgaaaaag cttttccagt ctgcaaattt aagataatgc
      241 gattggcgat cgatccgttc accgtaagag cgaagttcgc gcgggagcgc atccacggaa
      301 ttctaggaag gatttttctc tttgttcgaa atgacaatca cgagtacgag ttctatacgt
      361 atatgcgaat cgctttgcgg tcggcccaac cagatcatcg tttcttgagc gagttttaca
      421 gatgtgtaac tgtggcggca aaaatcgctc aggaggtcgt catgcgctat gagctttttt
      481 atcagccctc gatccaaggc cgcggcgaat ttgttcggtt gccgctggaa gatgccggcg
      541 cggaatatat cgatgtggcg cgcgatccca actttggccg gcccggaatt atgaaatttc
      601 tcgaagaccc atctttagaa catccgccgt tcgccccgcc gttcctgaaa gcaggaaagc
      661 tcctgatttc gcaaaccgcg aatatcttgc agttccttgg cccccggctc gatctcgtcc
      721 caaaaagcga agcgagtcgg atctgggcga accagctaca gctgaccatc gccgattggc
      781 tctatgaggc cgggcagacg catcacccca tcgccaatgt gctttactac gaggagcaaa
      841 aagaggaagc gaaaaaacgc gccggccact tcattgccaa tcgcatcccg aagttcctcg
      901 gctattttga aagaattctc aaacagaacg cgaatggcgg cgattttgtt ttcggtaaaa
      961 gagtctgcta cgtcgatctc tcactttttc agatgattga aggcttgcgc tatgcctttc
     1021 ccaaggcgat ggcgcgactg gagccgcaac atccccaaat ggtatctctg cacgatcgca
     1081 tcaaagcgcg gccgcggatc gcggcttacc tttcatcacc aaggcggctt gcgttcaacg
     1141 agcaggggat tttccggcat tatctggagt tggaagagag ctgaatcgga acaggtgcag
     1201 taagccgaaa cagcggcaat gaaacagtca gaggctttac cctactgatt catggaggtt
     1261 tcactagtcc acggagcatg aaatctccgc ttgcatccca gctcgtcacc cccgaacgtt
     1321 ttaatcgggg gtccagtccg aaaatcacct gtggtgagca cagtcgaacc accgctagaa
     1381 gcgtgcggga atgacggacg tgagaccgcc agcgttaacc cagcatctgt tttcaaggga
     1441 ggcgaaggac acgaacatcg tgacgtctgc gcacgtttgt tgcactttgg ctcacacgcg
     1501 catcaacatc gcggtgacag gcctgccggt cgccgcctcg atagcggcgg cgtaaagtcc
     1561 gacctggcgt cgataaacta tttcatgttc atgtaaatct tcatcggttt tgaaatcaat
     1621 gacaatccag cgctgatctt gctcgaatgc gaggtcgacg acaccctcgg taagaaagcc
     1681 atcgggatcg cgccacgtga ttggcgcttc gcgacggcag cggttttgtt tcgccgcact
     1741 ccgtgcccgg tcgaaaaccg aatgcgcaag ggccgtcgag acagcttcac ctgccgcagc
     1801 aatctcttcg gcggtggcac cgatgatacg gccatgtacg gttgcgagct ctcgaatcag
     1861 tgttaagtcg ccatcgagcg gtacgcttgc caaaaccgcg tgcaccaacg tgccgaaccg
     1921 tggtcccgag gggcggttcg tagcgcggtc cagctcgacg acttccacgg ccgacaccgt
     1981 cggcgtttgg cccgccgtcc cagctatttt cgccgattcg ataacggttc ggactgccat
     2041 cgagttctga gcgcccgtcg caatcgctgt atcccgctta ttgcgccagc ccttgtaagc
     2101 ggaaaggtcc gcttcgacgg tttgcggcgc gacatccttt acgatgaggt ccggtcgtcg
     2161 gatgccaagt ggcgcctcgg cgcccagctt gaggacgcgg ggatcccacc acacgaccga
     2221 aaaatcgccc actcctgcgt cttcccccgc gctccgcggg taaggagtaa ggagggcgtc
     2281 tggtttgagc atatgtagtc ccggagcaac cgtgttcgga ccggccgcgg tgccgccagg
     2341 ccgaactagc actgaatcgc tcgatctgaa ctccggtgcg cccggtggcc ctacttgctg
     2401 gcgcctcttt ccgatgggcg gataaatcgc gctgttgagc ggccggatcc aaccgtctct
     2461 ctcttcatcg cccactgccg ggatcaccag caggtcccgc gcacgggttg cggcgacata
     2521 ggcgacacgg gcgccctccg cttcgtcgcg gcgcaattct tcctgttcgt gctccagtaa
     2581 ctcagccggt gagcagccgg cgagacgtat ggcgcatatg ccacgatcag agtcgatcgt
     2641 acgactggcc acagagccgc gcagcttcgc cgtgatgtca gcaagaacga caacgggaaa
     2701 ttccaagccc ttggctcgat ggacggtcat caaacggacg ccatcgcttc cttcttcgag
     2761 aataggcgct tcgcctactt cgccgctctc ggcctgctgg cgcagctcgt cgatgaaacc
     2821 gcgaaaagaa attccgcctt cggcctcata gcgacgggcg agatcgctga catgcgccac
     2881 gttcgcaagc acctgctcgc cgccatgctc gagggcaaag ccaacgtgcg cgcgcgtgga
     2941 ttggagtaac tcggcaatgg ttgtggcaat cggcacccga ttgcgagagc gatggagtgc
     3001 ttgcagcttt tgcagtgctc tcacaatcgc agcgagattc tcgggtgctt tttcgggaat
     3061 ccgaaatgga tgaaaacggc caaacttatg acggtactcc agcagctcct catcgccgat
     3121 cgcgaacagg gatccacgca gggtcgcaaa gaccgacaac tcatcttccg gccattcaat
     3181 ggcggcgagg gcggcgcgca aggcttcgat ctcggcacga ttgtgaaagg agcgaccgcc
     3241 gacgagcaaa tgaggaatgc cgcgagcttc gagcgcctcg acgtatggcc gggtcatgtc
     3301 ttcttgatag ctgatgaaac ggcgaaacag cacgcaaaca tgccgagctt gaatcggaac
     3361 gaggtgctca gtttcaccgg ggcgaaaacc cagcgccgcg ctgggtgcca gcgaagcttt
     3421 cgctagcgcc ggcctctcgg cgacttgcca accgctttcg ttaatgagcc attggatgaa
     3481 cgccccgacc gcatcgggaa gagacttctc gatcgcgccg ttggaaattc gacgataacc
     3541 gtacggctcc ggcaccggta gtgctatgac gcagggctgt tccgctcgat ccaaccgaaa
     3601 aggctccagc ggtacgtatg ctgc
//
LOCUS       DASULZ010000317         2879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1156386, whole genome shotgun sequence.
ACCESSION   DASULZ010000317 DASULZ010000000
VERSION     DASULZ010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156386"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /gene="thiI"
                     /locus_tag="VFY96_06590"
     CDS             complement(<1..982)
                     /gene="thiI"
                     /locus_tag="VFY96_06590"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HEX5606162.1"
                     /translation="MDSVLIRYHEIALKKGNRRYFTELLKRNLLASVKDFGPKEIRSL
                     PARILVTFKNHPRSADLIERISGVFGVANFSLVERVDPDLEALRRHILESLNGHSFES
                     FRIETQRGDKTFPLGSPEINRQLGAAVKEKSGARVDLEHPDFTVTVEILPNDAFFGFN
                     KIAGAGGLPVGSSGRVVALISGGIDSPVAAYRLMQRGCRIFFIHFHSVPYQDRTSQEK
                     VRRLVQRLTRHQFSSRLYLVPFGEIQRQIVATVARPLRVVLYRRMMLRIAEAIARKEK
                     AKALVTGESLGQVASQTLDNMSVIQQAARLPILRPLVGMDKQEIIDQARRI"
     gene            1064..1390
                     /locus_tag="VFY96_06595"
     CDS             1064..1390
                     /locus_tag="VFY96_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017951937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5606163.1"
                     /translation="MTHFKKLADAMQFAPEKMKKNAVFETDRFFCDTYCFEPGQEQTP
                     HTHDGQDKIYYILEGRGFFKVGDEERELGPGEIALAPAGENHGVFNRGQHRLVTLVFV
                     TPKPHH"
     gene            1396..1818
                     /locus_tag="VFY96_06600"
     CDS             1396..1818
                     /locus_tag="VFY96_06600"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5606164.1"
                     /translation="MAPDEQEVAAANERFYRAFESLDIQKMESVWASDSEIQCGHPGW
                     RILRGWKPVMESWRRIFENTPAIRFMLTDIAIEVRSDLAWVTLYENLNSSLEGQNVAA
                     TILTTNIFRKGSDGWRMIHHHGSSVAQAAPQDETPTVH"
     gene            1837..2556
                     /locus_tag="VFY96_06605"
     CDS             1837..2556
                     /locus_tag="VFY96_06605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5606165.1"
                     /translation="MTALDGRVALVTGAGRGIGRAVALTLAQSGCRVILSARTVVQLQ
                     AVEREITNHNGTACALPADLTDDAAVQTLAAESRRIFGTVDVLINNAGWGKRAPVAKA
                     KIEDLDQTFRLNLRAPMMLARELLPDMIAKGEGAVINIGSVSGKTGEANGAAYSASKF
                     GLIGFTQSLYEEVREHGIKVSVILPGFVDTPLIPPNRQLDRSKMIQPSDIAHAVYYVL
                     TSAATCCPVEITVRPQRTPYR"
BASE COUNT          671 a          775 c          826 g          607 t
ORIGIN      
        1 cgatgcggcg ggcttgatcg atgatttctt gcttatccat gccaaccagc gggcgcagga
       61 tcggcagtcg cgccgcctgt tgaatcaccg acatgttgtc gagagtctgc gaagccactt
      121 gaccgaggct ttcaccagtc accagagcct tggccttttc cttgcgcgca attgcttctg
      181 cgatgcgcag catcatgcgg cggtatagca ccacgcgcag cggacgggca acggtggcga
      241 cgatctggcg ttgaatttcg ccgaagggca ccaggtaaag acgcgatgaa aattgatggc
      301 gggtaagccg ttgcacgagc cggcgcacct tttcctggga agttcggtcc tgataaggga
      361 cgctatgaaa gtgaataaaa aatatccggc aaccgcgctg catcaagcga taagccgcga
      421 ccggcgaatc gatgccaccc gagatgaggg cgacgacgcg tccgctcgac ccgacgggca
      481 ggccgccggc gccggcaatt ttattgaaac cgaaaaaagc gtcgttcggc agaatttcca
      541 cggtcacggt aaagtcggga tgttctagat ccactcgcgc gcctgatttt tccttcacgg
      601 cggcgccgag ttggcgattg atttcgggcg atccgagcgg aaaggttttg tcaccgcgct
      661 gtgtttcaat gcgaaaggat tcgaagctgt ggccgttcag cgactccaag atatgacggc
      721 gcaaagcctc gaggtccgga tcgacgcgct cgacaaggga aaaattcgcg acgccgaaga
      781 caccgctgat ccgctcgatg agatctgctg acctaggatg atttttgaaa gtaaccaaga
      841 ttcttgccgg cagactgcga atttctttgg gaccgaaatc cttgacgcta gccaataggt
      901 ttcgcttgag caactcggtg aaatatcgcc gattcccctt ctttaatgct atctcatgat
      961 agcggatgag gaccgaatcc atcacctaca gataacaaat gagcgctgct tttgcatgcc
     1021 ggcaccctat ggtaataata tccgtcatga acaggagcag ggcatgactc atttcaaaaa
     1081 gcttgccgat gcgatgcagt ttgcgccaga gaagatgaaa aagaacgcgg tcttcgaaac
     1141 cgatagattc ttttgcgata cttactgctt cgagcccggg caggagcaaa ctccccatac
     1201 ccacgacggt caggacaaaa tctattacat tctcgaaggc cgtggtttct ttaaggtggg
     1261 tgatgaggag cgcgaattgg ggcctggaga aatagcgctt gcgccggcag gtgaaaatca
     1321 cggtgttttt aaccgtggcc agcaccggtt ggtaacactc gtgttcgtta cgccgaaacc
     1381 ccaccactag cgttcatggc ccctgacgaa caagaagtcg cggcggcgaa tgagcgcttt
     1441 taccgcgcat tcgaaagcct cgacattcaa aagatggaga gcgtatgggc tagcgatagc
     1501 gaaattcagt gcggccatcc tgggtggcgc attttacgcg gctggaaacc ggtgatggaa
     1561 agctggcgcc gcatcttcga gaacacgccg gcgatacgct ttatgctcac cgatattgcg
     1621 atcgaagtcc gcagcgatct cgcctgggtg acactctacg aaaacctcaa cagttccctt
     1681 gaaggtcaaa acgttgcggc cacgattctc accactaaca tttttcgcaa gggaagcgac
     1741 ggctggcgca tgattcatca tcatggctcc tcggtggcgc aggctgcgcc acaagatgag
     1801 acgccaaccg tgcattgatc cggaaaaatt gggcgcatga ctgctctgga tggaagagtt
     1861 gcgctggtga cgggcgcggg gcgcggcatt ggcagagccg tcgctctcac gttggcgcaa
     1921 tcgggttgcc gagtgatcct cagtgcccgc acggttgtgc aactccaagc agtggaacgg
     1981 gaaataacaa atcacaatgg gactgcatgc gcgcttccag cggatctcac ggacgatgcg
     2041 gcagtgcaaa cactcgctgc tgaaagccgc cgcattttcg gtacggtcga tgtcttgatc
     2101 aacaatgccg gatggggaaa acgcgcgccc gtcgccaagg caaagatcga ggacttggac
     2161 caaacctttc ggctcaattt gcgcgcgccc atgatgctgg cgcgggagct cttgccggac
     2221 atgatcgcga agggcgaagg cgcggtcatc aatattggtt cggtttcagg aaaaaccggc
     2281 gaagccaacg gggcagctta ttccgcatcc aagttcggcc tgatcggatt tacccagtcg
     2341 ctctatgagg aagtgcgcga gcacggcatc aaggtatcgg taatccttcc gggcttcgtc
     2401 gacacgccgt tgattccgcc gaatcggcag ctcgaccgca gtaagatgat ccagccgagc
     2461 gacattgcgc atgctgttta ttacgtgctc acgtccgccg cgacgtgttg cccggtggaa
     2521 atcacggtca gaccgcagcg cacgccgtac cggtaatcta attgttcaag ccattcaaag
     2581 cgttcgatcg gttcgaccag ttgaagccct ctccgtcctc ccccgcgttg agcgtaaaac
     2641 acgaaaactc tcggctatca tgctgagtgg aacgaagcat ctcgtcttgt cggcgagtta
     2701 acaagacgcg attcttcgcc ttcggctcat cacaatgaca gttgcggcac agcagtcgag
     2761 aggaagagtc gggtttgaaa cgatttgaac gttttgaacg ttttgaactt cgtcactgac
     2821 agagccggtg caggaacggc agtgcgactt cttcgaggct ggcaacgatc gcatcggct
//
LOCUS       DASULZ010000318         2135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1460273, whole genome shotgun sequence.
ACCESSION   DASULZ010000318 DASULZ010000000
VERSION     DASULZ010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460273"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2087)
                     /gene="uvrA"
                     /locus_tag="VFY96_06610"
     CDS             complement(<1..2087)
                     /gene="uvrA"
                     /locus_tag="VFY96_06610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX5606166.1"
                     /translation="MQVIVDRFALRDSNKKRIVDSLEQAFHYGKGRLNLFLPPEDWRC
                     EPFSNHLHCPHCEIAYRDPVPNLFSFNSPLGACETCRGFGRIIDIDLDLVLPDPGKSL
                     SDGAIKPWAHRKRRTRRLLDFCERKQIPTKRPWGELTAKQKALVVEGDGEYKGIRGWF
                     RRLERKSYRMHVRVLLARYRAYLMCPECRGTRLKSDALHYRIEGKDIAQINAMSIGAA
                     HAFFQALKPAGALDQVGSLILDEIRRRLGYLVGVGLEYLTLDRQSRTLSGGELERVDL
                     TTAIGSSLVNTLYVLDEPSIGLHPRDSRRLVEILQRLRANQNTVIVVEHDPEIIKECD
                     HIIDLGPKAGEQGGEVVFAGSYQDLLNSEESLTAAYLSQRKTIPLPSRARKPLPQRWI
                     KITGARANNLKHIDVEIPLGLLVCITGVSGSGKSSLVDEVLYRNLRKLKEAPTAGVTD
                     CAKITGVDKISEVVLVDQSPVGTTPRSNPATYMKAFDGIRRLFAAADLSRLRGYTPST
                     FSFNVEGGRCETCRGEGFEKVEMQFLSDVYTSCAECHGSRFREEVLEVTYRGKNIRQI
                     LDLTVVESLEFFKDTADVREALAPLRAVGLEYVRLGQPMTTLSGGESQRLKLAAHMAK
                     ARKTGTLFIFDEPTTGLHFHDIERLLWAFNELIEQGHSIVVIEHNMEVIKCADYVIDL
                     GHIPAGRPQQNIAP"
BASE COUNT          457 a          611 c          616 g          451 t
ORIGIN      
        1 ggggcaatat tctgctgtgg gcgacccgcg gggatatgcc cgaggtcaat aacgtaatcg
       61 gcacacttga tgacttccat attgtgctcg atgacgacga tcgaatggcc ttgctcgatc
      121 agctcattga acgcccagag cagtcgctcg atgtcatgga agtgcagccc ggtagtgggc
      181 tcatcgaaga taaatagcgt gccggtcttg cgcgctttgg ccatgtgagc ggcgagtttc
      241 aagcgctgcg actcaccgcc ggaaagggtc gtcatcggtt ggcctagccg cacgtattca
      301 aggccgaccg cgcgcagggg cgccagcgcc tctctgacat ccgcggtgtc tttgaaaaat
      361 tccaaactct cgacgacggt cagatcgagg atctgtcgga tattttttcc ccggtaagtg
      421 acctccaaaa cttcctctcg gaagcgactg ccgtggcatt ccgcacagga ggtgtagacg
      481 tcggagagaa actgcatttc gactttttcg aacccttcgc cccggcaagt ctcgcagcgc
      541 ccgccctcga cattgaacga gaacgtcgat ggcgtgtagc cgcgcagccg tgacagatcg
      601 gcagcggcga acagacggcg aatgccatcg aaagctttca tataagtcgc gggattcgag
      661 cgcggcgtcg taccgaccgg cgactgatcc acgagtacga cttcggagat cttgtcgacg
      721 ccggtgattt tcgcgcaatc cgtcacgccg gcggtcggag cttccttgag ctttctcaaa
      781 ttgcgataaa gcacttcatc aacgagactg gatttacctg atcccgacac gccggtgatg
      841 cacaccagca aacccaaggg aatctccacg tcgatgtgct tgagattatt ggcacgagca
      901 ccggtgattt taatccaacg ctgcggcagc ggcttacgcg cacgcgaggg gagcggaatc
      961 gtcttgcgtt ggctcaagta cgccgcggta agggactctt cgctgttgag caaatcctgg
     1021 taggagccgg cgaataccac ttcgccgccc tgttcccccg ccttcggccc caagtcgata
     1081 atgtgatcgc attctttgat gatctcagga tcatgctcga cgacaattac ggtgttctgg
     1141 ttggcgcgga gccgttgaag aatttcgaca agtcgtcggc tatcgcgtgg atgcaggccg
     1201 atagacggct cgtcgagcac gtaaagcgtg ttgaccaacg aggagccgat cgcagtagta
     1261 agatcgacgc gttcgagctc cccgcctgaa agtgttcggg attgccgatc gagggtcagg
     1321 tactcgagac cgacgccaac gagataaccc aaacggcgac ggatctcatc caatatcagg
     1381 cttcccacct gatccagagc accggcgggc ttgagcgctt gaaaaaaagc atgcgctgcg
     1441 ccgatactca tggcgtttat ctgcgcgatg tctttccctt cgatgcgata atggagcgcg
     1501 tcgcttttca gccgcgtgcc acgacactcg gggcacatca gataggcgcg gtagcgcgct
     1561 agcaaaaccc gaacgtgcat gcggtagctt tttcgctcga gccggcggaa ccagccacgg
     1621 atgcccttgt attcaccgtc accctctacg accagcgcct tctgtttcgc cgttagttcg
     1681 ccccagggcc ttttggtcgg aatttgtttg cgctcgcaaa aatcaagtaa gcgtcgggtc
     1741 cggcgctttc gatgggccca cggcttgatc gcgccgtcgc tgagcgattt gcccgggtcc
     1801 ggcaacacca gatcgagatc gatatcgatg atccggccaa agccgcggca ggtttcacag
     1861 gcgcccagag gactgttgaa cgaaaagaga ttcggcaccg gatcgcgata ggctatttca
     1921 caatgcgggc aatgcaagtg attgctgaac ggctcgcagc gccaatcttc cggcggcagg
     1981 aacaagttca agcggccctt gccgtaatga aacgcttgct caagagaatc cacaatgcgt
     2041 tttttgttgg aatcgcgcag cgcgaagcga tcgacgatga cttgcaaggg ctcgcttccc
     2101 ggaggagcct cggtcgattc gtcaatttcc agcac
//
LOCUS       DASULZ010000319         2636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1054025, whole genome shotgun sequence.
ACCESSION   DASULZ010000319 DASULZ010000000
VERSION     DASULZ010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054025"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1195
                     /locus_tag="VFY96_06615"
     CDS             191..1195
                     /locus_tag="VFY96_06615"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606167.1"
                     /translation="MHLRLRLCGSALIILIIFLHSTAVAQDRPLKKITWGMTALTASM
                     WIPWLAKEARIYEKNGLDVDIILLRGSGQSSQALLGGSLFAAPVALPQVMLADLNGAD
                     LVNVAHTIAVASSKLVVKPEIKKPEDLRGKKIATSSIGSLGDFLFRYALRKYGVNPDR
                     EITWLSIGTNAERLQALLTGAIDGADVAYPADVQAERKGYHVLIDARKEITFPTTSVV
                     TRRKTIREDRDAVMRFVRSHVEAIAFHKQNKEFSLRVLTKYVRTTDPEFLEGSYNIFK
                     QDFIAAPYPITKGLEPTYDYVAQTRPEIRTHKPEEFVDPSFIAELDKSGFIKRLYEQK
                     "
     gene            1333..2451
                     /locus_tag="VFY96_06620"
     CDS             1333..2451
                     /locus_tag="VFY96_06620"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5606168.1"
                     /translation="MMKILAILVLFVGLNSLRSGASIAAESKRLPDWDKALADAKIEG
                     KIVLAIPPATELRTALEPLLKQKFGLEAELVSAPGPKNASRIAAEKKAGVNYFDALIC
                     GTGTAIGLGYDGVLEPIESFWILPEVKDSNQWWGGHIWEDNLTSRKFLYSFLADVSTH
                     STWYNTSLAKPQELRSFDDYLSPKWKGRIGFSDPRVPSSGQSIWSFMWEQRGEEFLKK
                     LVEQDLFLTRDLRQLADALAKGKVALAFGLGRSQTEPFVKAGLPIKPAPVTKEGLPAS
                     NAFGVLGVIKDPPHVNATKVFVNWFLGREGQDWYSRIMQNGTRRLDVNTRWLKELGIS
                     AAKDALTVQEYHRVRNHLEDKYTKVRLPAGKFAETILH"
BASE COUNT          645 a          680 c          721 g          590 t
ORIGIN      
        1 tctggaagca acttacgact atgtcgcgca aacacgaccc gagattcgta accggaaacc
       61 tgacgatttc gtggatccga gcttcatcgc cgagttggac aagagcggtt ttatcaaggg
      121 gctgtacgaa ggcaactagc caacggacag cagccaaaac gtaacaatca tttcggaaca
      181 aggattgcgt gtgcatttgc gcctgcgact ctgcggatca gcattaatca ttctcatcat
      241 ttttctacac tccacggccg ttgcccaaga tcgtccgctg aagaaaatta cctggggcat
      301 gaccgcgttg acggcgtcca tgtggatacc ttggctggct aaggaagcaa gaatttacga
      361 gaagaacggg ctcgacgtgg atatcatctt attgcggggc tcggggcaga gctcgcaggc
      421 gctcttgggc ggcagcttgt ttgctgcgcc ggtggccttg ccgcaggtga tgctcgcaga
      481 tctcaatggc gccgacttgg tcaatgtcgc tcatacgatc gccgtggcga gcagcaagtt
      541 ggtagtaaaa ccggagatca aaaagccgga agatctccgc ggcaaaaaga tcgcgacttc
      601 gagtatcggc tcgctcggtg attttttgtt ccgctacgca ctgcgcaagt atggcgtaaa
      661 tcctgaccgc gagattacct ggctttcgat cgggaccaat gcggagcgcc tgcaagcttt
      721 attgacgggc gcgatcgatg gcgcggatgt cgcttatccc gctgacgtac aggccgagcg
      781 caagggatac cacgtgctga tcgacgcgcg gaaggaaatc acgtttccga ccacgtccgt
      841 ggtcactcgc cgtaaaacca ttcgtgagga tcgcgatgcc gtgatgcgct tcgtgcgctc
      901 acatgtcgaa gccattgcct ttcacaaaca gaacaaagaa ttcagtctga gagttttgac
      961 caagtacgta agaaccaccg atcccgagtt tttggaaggc tcctacaata tattcaagca
     1021 ggacttcatc gccgcaccct atccgatcac caagggactg gaaccgactt atgattacgt
     1081 cgctcaaacc cggccggaga tccgcacaca caaaccggaa gagtttgtcg acccaagctt
     1141 tatcgcggaa ctggataaaa gcggatttat caagcggctt tacgaacaga agtaaattga
     1201 gcggcggtgc ggtaagcttc attcaattcg aaacatcacc cgccctccgt tctataaatt
     1261 acagcggcag gacatgcttc gcatttgcct tgcgtctaca gctcggcaac catttcgcac
     1321 ccactggatt ccatgatgaa aatattggcc atactcgttc tgttcgttgg attgaactcg
     1381 ctgagatcgg gtgcctccat tgccgctgaa tccaagagac ttccggactg ggacaaggcg
     1441 ttagcagacg caaagattga aggaaagatc gtgctggcga tcccgccggc cacggagcta
     1501 cgcacggcgc tcgagccttt actgaagcag aaattcggcc tcgaagctga gttggtctct
     1561 gcacccggac cgaagaatgc gagtcgaatt gcggccgaga aaaaagccgg agtgaattat
     1621 tttgacgcgc ttatctgcgg cacgggtacc gcgatcggac ttggttatga cggcgtgctc
     1681 gaaccgatcg agtctttttg gatccttcct gaagtcaaag attcaaacca atggtggggc
     1741 ggtcatatct gggaggataa tcttacgagc aggaaatttc tttattcgtt tctcgccgat
     1801 gtgagcacgc atagcacttg gtataacacg agcttggcca agccgcagga actccgttca
     1861 ttcgatgatt atctgagccc gaaatggaag ggacgtatcg gttttagcga tccacgcgtg
     1921 cccagctccg gccagtcgat ctggtcattc atgtgggaac agcgcgggga ggagtttcta
     1981 aaaaagctgg tggagcaaga tcttttcttg acccgcgatt tgcgtcaatt ggccgatgcc
     2041 ctcgccaaag gaaaagttgc gctcgctttc ggtctcggcc gcagccaaac cgagccgttc
     2101 gtcaaggctg ggctgccgat caagccagcg cccgtcacca aggaaggctt acctgcgagc
     2161 aatgccttcg gtgtgttggg cgtaatcaaa gatccgcctc acgtgaatgc gacaaaggtc
     2221 ttcgtcaatt ggtttctcgg ccgagaaggt caggactggt atagccgaat catgcaaaac
     2281 ggtacgcggc gcctcgacgt gaatacgaga tggttaaaag agcttggcat ctccgccgcc
     2341 aaggacgctc tgaccgtgca agaataccat cgcgtgcgaa atcatctcga agataagtat
     2401 accaaggtgc gattgccggc gggcaagttc gctgaaacga tactgcacta atggttcacc
     2461 atgcgttgtc gatgattgca ggaaggaaca tccttgcgca atactgcgaa ggccattttc
     2521 aggttaagcg gcccgcgtct aacgcgtgtt gttacaggtc attatcttga caagggaaga
     2581 agctcagaat agcttcgcgt cagcatcaca ctgtttttga aatcatggag aaatcg
//
LOCUS       DASULZ010000320         4858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1360569, whole genome shotgun sequence.
ACCESSION   DASULZ010000320 DASULZ010000000
VERSION     DASULZ010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360569"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VFY96_06625"
     CDS             <1..714
                     /locus_tag="VFY96_06625"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX5606169.1"
                     /translation="DYLRRIDQLAREVADRRDPAGDVYHSLAALNHVLFRQHGFDGNR
                     DDYYDPRNSFLSEVLDRKIGIPISLSVLYMEVARRISVPVEGIGFPGHFLVKCNDGEE
                     RIFIDPFHRGEIKLTEDLGQMLEKLYGRKVALRPQFLEGTGKKQILKRMLANLKAIYL
                     RNSSIGEALSIFDHLIILDPAGAEDIRDRGSVYLRMGCFRQARDDFEAYLRLAPNAHD
                     AAMVREQISNLEKQPRLLH"
     gene            complement(711..2942)
                     /locus_tag="VFY96_06630"
     CDS             complement(711..2942)
                     /locus_tag="VFY96_06630"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5606170.1"
                     /translation="MQERINKFHIVLIKPSKYDDDGYVVRFWKGVLPSNTLNVLHGLT
                     EDIRQRRVFGELPIEVTTFDETAEKIPMAKIVRWSKTAGVKLLVCLVGVQTNQFPRAL
                     DMARFFKRHGLSVIVGGFHTSGTINMLGDQEPDIQQLYREEICMVSGEVEGKWEGILA
                     DFLHDRLKPLYSFAQDLQNLVDIGEAPLPQTSAKTMKHFAYPSFATADTSRGCPFACT
                     FCTIINVQGRKMRERNPQSIAAMMRRNYLEHGLSFYFFTDDNFARKKLWRETFEEIIK
                     LREEGLDVSFMMQVDLAKKPKDFVTLAAKAGCTQVFIGMESVNPQNLEAEGKKQNKVD
                     EYRDIIEEWHDAGVLVHTGYIIGLPWDSQEQVAQDMRFLIDEVGPDQASFFMLTPLPG
                     SHDHREMKKRGEWMDPDFNKRDSFHATIKHPLMSAEEWTGAYENAWKTFYSKENMIRN
                     LARWVDRPKNYWNLMSIFFWYKNAALIEKQHPMIAGFFRLKDRKNRRPGFAIDPVHVH
                     LWKRAKEVTRLLITWTKFVKEMEEVWLQTRKKTETEERWLEQIQKIQSDIWQALRIGE
                     IQKIYNNAKETLPERARALLDPLEDLSSRVVYSRNDLNRFLRQWDALRVKIQELRPRE
                     GEAVRRLLDDIHHIQASIRFGDRIHEWQDAYTRLRENLPSRPQLSFITFDAINHRIVY
                     SRQGLERMWSQSLRNLRRFKLSKIHPWRCATAVAKEFSLTTSFASSFLQFSKQSGSEF
                     GPG"
     gene            3149..3790
                     /locus_tag="VFY96_06635"
     CDS             3149..3790
                     /locus_tag="VFY96_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5606171.1"
                     /translation="MADINLEIDERELVFICGPSGAGKSTLLKLLFREIDPSEGEILI
                     DGRNVTRLRSRGVARLRRKMGLVFQEFRFLPALNVLDNVALAAQVLGVSKKQSRVKAY
                     QLLRELGLNDRYDAKPFMLSGGEQQRVAIARALINDPSVIIADEPTGNIDHELTDGIM
                     QLFLKIRDGGAAVVIATHDRALIQRYGARVIKLERGFVADAWPGNITMPEYTW"
     gene            3859..4710
                     /locus_tag="VFY96_06640"
     CDS             3859..4710
                     /locus_tag="VFY96_06640"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEX5606172.1"
                     /translation="MLTSLTMALTLFVFGAFMLLQANLEQLLTALGDQMHITAYLGPA
                     LEPTALERLLKSVEALPEVERVRYTSQTQAWNDFQTALGAQSGLLEGLPHDVLPASIE
                     ISIKRGQRNSSTVEQLAQWLKGEKEIASVEYPQQWLERLALTVLAVAWAKWIVGGIFF
                     LATFFIVGSTVKLALYARRGEVEIMQLVGASETLIQAPFVLEGMLQGMTGAALALAAL
                     WGGYALMHHEIESFAAAVWSFGHARFLDFEGAVFLIATGWILGAAGSLISLRRIVKTW
                     RGSEVTV"
BASE COUNT         1074 a         1259 c         1343 g         1182 t
ORIGIN      
        1 gactatctgc ggcgcatcga tcagctcgcg cgggaggttg ccgatcggcg cgatccagcg
       61 ggcgatgttt atcattcgct ggccgcattg aatcacgtat tgtttcgcca acacgggttc
      121 gacggaaacc gcgacgatta ctatgatccg cgtaatagtt ttttgagtga agtgctggac
      181 cgaaaaatcg gtattccgat cagcctatcc gtcttataca tggaggtcgc aaggcgaatt
      241 tctgtgccag ttgagggtat aggttttccc gggcacttcc tcgtaaagtg taacgatgga
      301 gaagagcgta ttttcatcga cccattccat cggggcgaga tcaagctgac cgaggacctt
      361 ggccaaatgc tcgagaagct atacggcagg aaagttgcgc taagaccgca gtttctcgag
      421 gggacgggca aaaaacaaat tttgaaacgg atgcttgcca acctcaaagc gatttacctg
      481 agaaatagct cgattggcga ggcgctgtcg atatttgatc atttaatcat tctcgatccg
      541 gccggagctg aggacatccg cgatcgcggc tccgtctatc tgcgaatggg ctgcttcagg
      601 caagcgcggg acgactttga ggcgtatctt cggctcgcgc ctaatgcgca cgatgcggca
      661 atggttcgcg agcagatttc caacctggaa aaacagcccc gtctactgca ctaacccggg
      721 ccgaattcag agcccgattg cttcgaaaac tggagaaagc tactggcaaa ggaagtggtc
      781 agtgaaaatt ccttggccac cgccgttgcg cagcgccacg ggtgaatctt cgacagcttg
      841 aaacggcgca ggttacgcag gctctgtgac cacatgcgct cgagtccctg ccgagagtag
      901 acgatgcgat ggttgatggc atcgaaggtg atgaagctca actggggtct ggagggcaaa
      961 ttctcgcgta accgagtgta cgcgtcttgc cattcatgaa tgcgatcgcc gaaccgtata
     1021 ctcgcctgaa tgtgatgaat gtcgtccaag agccggcgca ccgcttcgcc ttcgcgcggc
     1081 cgcaattcct ggatttttac acgcaatgca tcccattgcc tcaagaatcg gttcaaatca
     1141 ttcctgctgt acaccactcg tgacgagaga tcctccaagg gatcgagcaa agctcgcgct
     1201 cgctcaggca aggtttcctt ggcattgttg taaatcttct gaatctcgcc gatgcgcaaa
     1261 gcctgccaaa tgtccgattg aatcttttga atctgttcga gccagcgctc ttcggtttcg
     1321 gtttttttgc gcgtctgcag ccaaacttcc tccatttcct tgacaaactt ggtccaggtg
     1381 atgagcagcc gggtgacttc tttggcgcgt ttccagaggt gcacgtgaac cggatcaatc
     1441 gcaaacccgg gtcggcggtt tttacggtcc ttcagacgga aaaagccagc aatcatcgga
     1501 tgctgcttct caatcagcgc ggcattctta taccagaaaa agatcgacat cagattccag
     1561 tagttttttg gccgatccac ccagcgggcg aggttccgaa tcatgttttc tttgctgtag
     1621 aacgttttcc aggcgttctc atacgctccc gtccactctt cggcactcat gagcggatgt
     1681 ttgatggtgg catggaagga atcgcgcttg ttgaaatcgg ggtccatcca ctcgccgcgt
     1741 ttcttcattt ctcgatggtc gtgggagccg ggaagaggcg tcagcataaa aaacgatgcc
     1801 tgatcgggac cgacctcgtc gattagaaaa cgcatgtctt gcgcgacctg ttcttgggag
     1861 tcccagggca gcccgataat atagccggtg tggaccaaga cgcccgcatc gtgccactcc
     1921 tcgatgatat cgcgatattc gtcgactttg ttttgttttt tgccttcagc ttcgagattt
     1981 tgcgggttga cgctttccat accgatgaaa acctgggtgc aacccgcctt ggctgccaaa
     2041 gtgacgaagt ccttcggctt cttagccaga tccacctgca tcatgaacga cacgtcgagc
     2101 ccttcctcgc gcaacttgat aatttcctcg aatgtctcgc gccagagttt tttgcgcgcg
     2161 aaattgtcat ccgtaaaaaa gtagaacgag aggccgtgtt caagataatt gcggcgcatc
     2221 atcgcagcga tgctttgcgg gttacgctcg cgcatttttc ttccctggac attgatgata
     2281 gtgcagaacg tgcaggcaaa tggacaaccg cgagaggtat cagccgtggc gaaactcggg
     2341 taggcaaaat gtttcatcgt ctttgcgctc gtttgcggca gcggtgcctc accgatgtcg
     2401 acgagatttt gcaagtcctg ggcgaagctg tagagcggtt tgagacgatc gtggagaaaa
     2461 tcggcgagta tgccctccca ttttccctcc acctcacccg ataccatgca gatctcttcg
     2521 cgatagagct gttgaatatc gggttcctgg tcaccgagca tgttgatggt accgctggta
     2581 tgaaatccgc cgacgatgac cgaaaggccg tgccgcttaa aaaatctcgc catatcgaga
     2641 gcgcgaggaa attgatttgt ttggacgccg acgagacaca cgaggagttt gacaccagcc
     2701 gttttactcc aacggacaat ctttgccatc ggaatttttt ccgcggtttc gtcgaacgtg
     2761 gtcacttcga tcggcagttc gccgaaaacg cgccgctgtc ggatgtcttc cgtgagcccg
     2821 tgcagtacgt taagcgtatt actcggcagt acgcctttcc aaaagcgaac cacataaccg
     2881 tcgtcatcgt atttagaggg cttgatcaaa acgatatgga atttgttgat acgctcctgc
     2941 atcggttctc tcctgcgaat gcttgtaagt tgggcgtcca tcttttatag atagcgagga
     3001 taaacggagt cttggaaact ctaacaaccg ccctcctgca tgtcaaggta gaactgcttt
     3061 taaatatcga ttctcactgg gaaattttac agttttgagg atccagctgc gacacgtctc
     3121 gaaaacattt ccaccgcgcc aacgggcgtt ggccgatatc aatttggaaa ttgatgaacg
     3181 cgagttggtt ttcatctgcg gtccgagtgg cgccggcaaa tcgacgcttt taaaactgtt
     3241 gttccgcgag atcgacccga gcgaaggaga gatcctgatc gatgggcgta acgtgacgcg
     3301 gttacggagt cgcggcgttg ctcgattgag aagaaaaatg ggcctagtct tccaagaatt
     3361 caggtttctg cccgctctca atgtgcttga caacgttgcg cttgccgctc aggtgctcgg
     3421 agtttcaaaa aaacaaagcc gggtgaaagc ctaccagctg ctcagggagc ttggcttgaa
     3481 cgatcgctac gacgccaagc ctttcatgct ttccggcggc gagcaacaaa gggtcgccat
     3541 cgctcgtgca ctgatcaatg acccttcggt gatcatcgcc gacgaaccca ccggcaatat
     3601 cgaccatgaa cttacggacg gcatcatgca gctttttctg aaaatccgtg acgggggtgc
     3661 cgcagttgtg atcgcaactc atgatcgggc cttgatccag cgttacggcg cgcgcgtaat
     3721 caagctcgag cgcggatttg tcgccgatgc ttggccaggt aatatcacca tgccggagta
     3781 tacctggtga aagtgtcgct cgttggattc gtttttcgac aggtgcgaag caatctccgc
     3841 catctatgtt ggagccacat gctgacctcg ctgacgatgg cgttgaccct gttcgtgttt
     3901 ggcgctttca tgctcctgca agcgaatttg gagcagctgc ttacggcctt gggtgatcaa
     3961 atgcacatta ccgcttattt gggccctgcg ctcgaaccga ctgcactcga gcgactatta
     4021 aagagcgttg aagctctgcc cgaagtggag cgggtgcgct ataccagcca gacacaagcg
     4081 tggaacgact ttcaaactgc cttaggtgcg cagtccggat tgctcgaggg tttgccccat
     4141 gacgttttgc cggcatcgat cgaaatcagc atcaaacgag gtcaacgcaa tagttccaca
     4201 gtggagcaat tggcgcagtg gcttaaaggc gaaaaagaga tcgccagcgt tgaatatccg
     4261 cagcagtggc tggaacggct cgctttgacg gtgctcgctg tcgcgtgggc caaatggatt
     4321 gttggcggga tctttttctt ggccactttt tttatcgtcg gcagcacggt gaagctggcg
     4381 ctgtacgcgc gccgcggcga agtcgagatt atgcagctgg tcggcgcttc ggaaacccta
     4441 attcaagcgc cgttcgtgct cgagggaatg ctgcaaggaa tgacaggcgc ggcactggcg
     4501 ctagcggcgc tctggggcgg gtatgctctt atgcaccacg agatcgaatc attcgccgct
     4561 gccgtatggt cgtttggcca cgcacgtttc ctggatttcg aaggagccgt ttttttgatt
     4621 gcgactggtt ggattctggg tgctgccggc agtctcattt ctctgcgaag gatcgtcaag
     4681 acgtggcgcg gttccgaagt gacagtttag ctctcgtact ggggattttc gcggttctac
     4741 cgagcacagc gcgcggcgcg gcggccaatc gggaactcga cgggatccgg aaaaagatcg
     4801 agaacgaaaa aaagggactt tcccagctcc aagtcagaga agggtcggtg ctcgattc
//
LOCUS       DASULZ010000321         5610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_750698, whole genome shotgun sequence.
ACCESSION   DASULZ010000321 DASULZ010000000
VERSION     DASULZ010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750698"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VFY96_06645"
     CDS             <1..804
                     /locus_tag="VFY96_06645"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5606173.1"
                     /translation="LRLYALEDAELQETCFQIANDWMIDYCNAAPGRLFGIPMISMYN
                     IPKAIKELERCKKAGMIGCMIWQVPPEHLPFTSDHYNPFWEAASELDMPVNVHILTGF
                     NYSRFERKGLDTYRMTVNQKLADAANILFDFVFGGVLAKNPKLKLVYVENEVGWIPFY
                     CHEWDKYFVRHSPKAPLPYMKKLPSEYVREQVYATFFSDPCGGRMMDWFGEDNFMWSS
                     DYPHAASTWPHSRQVIADELGHLPKSVIDKVVRQNVIDLYNLKVDGINN"
     gene            834..1316
                     /locus_tag="VFY96_06650"
     CDS             834..1316
                     /locus_tag="VFY96_06650"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5606174.1"
                     /translation="MHAARKLEPTEKPKTQKFSLRTPYMKQGRITQLVAETENMWLHT
                     KINYEGGENEIHTHLDEDHAFIVLEGRMSVFDEKGGEIKVEKHQGVMIPKGAYYRYLN
                     TGSENLVVLRVGAGVKGQKQGGEEMRVRPDGKPLIASSEENKTVAPIEDTRFFADTAG
                     "
     gene            1390..1950
                     /locus_tag="VFY96_06655"
     CDS             1390..1950
                     /locus_tag="VFY96_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606175.1"
                     /translation="MTQAVRPMPEEVPVELWRGVKPSAAVWQGRVVSIFVARKSAEEM
                     VSLSTVRAMADRGLEGDRFFRDSWSAIARSDKAVSFIEDEVLELAAEELGLESIAQKT
                     RRNIVTRGVPLVDLLHREFVVGDVRMRGIRLFEPCGHLVKISKLPGIFKALDHRSGLK
                     AAILTDGVISIGNRIALDELHQPAVG"
     gene            2166..3104
                     /locus_tag="VFY96_06660"
     CDS             2166..3104
                     /locus_tag="VFY96_06660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX5606176.1"
                     /translation="MDFGLHLPASSATVKPEDLVRFAQQAEALGFYCLTIADHVIVPK
                     NISVSYPYTVDGKYPGTGYHLETLTTMGYLMGATQRIRFATSVMILPYRNPIVTAKML
                     ASLDVLSGGRVIVGAGIGWMKEEFENIRTEPFSERGRVTDEYIAAFRELWTSENPSFH
                     GKYCRFSDIVFLPKPVQKPTIPIWIGGHSKQAIRRAARLGDGWHPIGGVPTIPLEPQD
                     IAADMSMLGEYAERAGRDAKKIRVALKGSLFDREKQITPGKRRRFIGSAEEVASDIRD
                     YRAVGVDTMIFDVRRPTIAETLERMEWMAKDVFPQV"
     gene            complement(3265..4110)
                     /locus_tag="VFY96_06665"
     CDS             complement(3265..4110)
                     /locus_tag="VFY96_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606177.1"
                     /translation="MADIRGVGKKITYSEDNPISAEVDALRKSRDNIKRPPKDDEERE
                     RLARWLAHRGRDELEITVGTACYAMAHFEMDCEWRYFLAAHAGTEAGHGWGYIRQANA
                     IDPSRDHAKPDPEFERKYGLTPRIEHHQIMKRDFLSYIFSGNLWPYGHVTAASIQSIQ
                     ITTPKLLDFEERVVHAEERSHHDAILQKIHDYVWELIAQYGEAPIRRRIAAIDAEALN
                     SRPRTVFDPPRREFLRKYFNVPVENVRKFPAWREYLYFNVLGFPPEPVYIENWPAEIP
                     QPKAA"
     gene            complement(4132..5157)
                     /locus_tag="VFY96_06670"
     CDS             complement(4132..5157)
                     /locus_tag="VFY96_06670"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606178.1"
                     /translation="MSAARTLMQVQNSFSCLTRSIRLFGFALCILADCRAIVWSADSL
                     RFGFSGATATQLAGAIAVEQKLFERYGVNVEFTQSAGTTMIRALDSGSLQLAIVGGGQ
                     ALSAYLKGVDVRIISGLVNAIPFQLWVKPEISSVKDLRGKLIANTPPGTSLNLANQVT
                     LARAGLDPSRDVKLVAFGRLGLVSQALFTGVVDAALLSPPETIEAKKSGLHMILDLAT
                     ARIPCPFTSVVTTRAMLEKEPFLVARSLKGILHGVNLALTNPDLAKKTISRTMRLADT
                     EAIEEVYQRVTMVYERFPIVSREAIDTILKLSPVTSNRNPYGVLDMSVLERIEKEGFV
                     RSLYAAK"
     gene            5309..>5610
                     /locus_tag="VFY96_06675"
     CDS             5309..>5610
                     /locus_tag="VFY96_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5606179.1"
                     /translation="MRHRVIHLINPKTDSLTTRPLYLNRALYSPLAGLLAVASAVPRD
                     RYEVVLTDENIEPIDFELEADLVGISAMTCYVKRGYEIADAFRKRGVPVVMGGVHP"
BASE COUNT         1313 a         1531 c         1527 g         1239 t
ORIGIN      
        1 cttcgccttt atgcccttga ggacgcggaa ctccaagaaa cttgctttca aatcgccaac
       61 gactggatga ttgattactg caatgccgcg ccgggtcggc tgttcgggat cccgatgatt
      121 tccatgtaca acatcccgaa ggccatcaaa gagctagagc gctgcaaaaa agccggcatg
      181 atcggttgca tgatctggca ggtaccgccc gaacacctgc cgttcaccag cgaccactac
      241 aatccattct gggaagccgc ctcagagctc gacatgcctg tcaacgtcca catcctgacc
      301 ggcttcaatt acagccgttt cgaacggaag gggttggata cctatcgaat gaccgtgaat
      361 cagaagcttg ccgacgccgc aaatattcta ttcgatttcg ttttcggcgg cgtgctggcg
      421 aaaaatccca agctaaaact ggtctatgtc gaaaacgaag taggctggat tccgttttac
      481 tgtcacgagt gggataaata tttcgtgcgt catagcccaa aggcgcccct gccttacatg
      541 aaaaaattgc cgagcgaata cgttcgcgag caggtctatg ccaccttctt cagcgatccc
      601 tgcggcgggc gcatgatgga ctggttcggt gaagacaatt ttatgtggtc gagcgactat
      661 ccacacgctg cctcgacctg gccgcactcg cgtcaggtca tcgccgacga gctcggccat
      721 ctgcccaaat cggtcatcga taaagtcgtg cgccaaaacg tcatcgatct gtacaattta
      781 aaagtcgacg gcatcaataa ttaacgaaat taaacaggtt gaaaggagcc atcatgcacg
      841 cagcgagaaa gttggaaccc acggaaaaac ccaagacgca aaagttttca ttgcgcaccc
      901 cgtacatgaa acagggtcgc atcacccagt tggtcgcgga gaccgagaac atgtggctcc
      961 acaccaagat taactatgaa ggcggcgaaa atgagattca cacgcacctc gatgaagacc
     1021 atgcgttcat cgtcctcgaa gggcggatga gcgtgtttga cgaaaagggc ggcgaaatca
     1081 aagtagagaa acatcaaggc gtgatgatcc ccaagggcgc ctattatcgt tatctcaata
     1141 ccggcagtga aaatctggtg gtgctgcgcg tcggcgcggg cgttaaaggg cagaagcagg
     1201 gcggcgaaga aatgcgggtc cgaccggacg gcaaaccgct aattgcatcc tcggaagaga
     1261 acaagactgt ggcgccgatc gaggatacca ggttttttgc ggacacggca gggtaagaag
     1321 ccgcgtcaag agagttgtta tacgccggcc actgcttttc agtggccggt ttcgttttaa
     1381 ggagccccta tgactcaagc ggttcgacca atgccggagg aagttccggt ggagctgtgg
     1441 cgcggtgtta agccgtccgc ggcggtctgg caaggcagag tggtgtcgat ttttgtcgcg
     1501 cggaaatcgg cggaggaaat ggtctcgctc tcaaccgttc gggcaatggc ggaccgcgga
     1561 ttggaaggcg atcggttttt tcgcgattcc tggagcgcca ttgcccggtc cgacaaagct
     1621 gtgagtttca tcgaagatga ggtgctcgag ctcgccgcag aggaactcgg actcgaatcg
     1681 atcgcgcaaa aaacccgacg taatatcgtt actcggggag ttccgctggt cgatttactg
     1741 caccgagagt tcgtggtcgg tgacgtccgg atgcgcggca ttcgtctttt cgaaccatgc
     1801 ggtcatttgg taaagatttc caagctgccg ggtatcttca aggcgctcga ccatcgcagc
     1861 ggactcaaag cggcaatcct gactgacggt gttatttcca tcggtaaccg gatcgcgttg
     1921 gatgagcttc accagcccgc ggttggataa ttcaagcaag tacttcctgt catctcgaac
     1981 gaagtgagag atctttctta aaccgactcg gagcaaatgc gatcataact cggtcgctca
     2041 agaaaaattt ctcgctctgg tcgaaatgac aatgagcctg ttgcggtcta cgtgcaggct
     2101 cgggctaggt ggcttgcaga cccagtaact cagttgagaa cggcgcgagg tcattcggat
     2161 caaccatgga ttttggactc cacttacccg catcgtcggc cacggtcaaa cctgaagatc
     2221 ttgttcgctt cgcgcagcaa gccgaagctc tcggctttta ctgcttgacc atagctgatc
     2281 atgttattgt gccgaaaaat atttccgttt cttatcctta cacggtcgac ggcaaatatc
     2341 ccggcaccgg ctaccacctc gagacactga cgacgatggg ttatctcatg ggggcaacgc
     2401 aacggattcg cttcgcgacc agcgtcatga ttttgcccta ccgtaatccg attgtgacgg
     2461 caaagatgct cgcaagcctc gacgtgttgt ccggcggtcg ggtgatcgtc ggcgcgggta
     2521 tcggctggat gaaggaagag ttcgaaaaca tccgcaccga gcccttcagc gaacggggca
     2581 gagtcaccga cgagtatatc gcggctttca gagagttgtg gacgagcgag aacccctctt
     2641 tccacggcaa atactgccgt ttttctgaca tcgttttttt gccgaaaccg gtgcagaaac
     2701 ccaccatccc gatctggatc ggtgggcata gtaaacaagc gattcggcgc gcggcacgac
     2761 ttggcgacgg ttggcacccg atcggcggcg tgccgacgat tcctctagag ccgcaagaca
     2821 tagcggcaga catgtcgatg ttaggggaat acgccgaaag agccggccgc gatgcaaaaa
     2881 aaattcgcgt cgcgctgaaa ggttcattgt tcgatcgcga aaagcaaatc actccgggca
     2941 aacgccgtcg ttttatcggc tccgccgagg aagtggcctc ggatatacgg gactatcgtg
     3001 ccgtcggcgt tgacacgatg atattcgacg tgcgccgccc gaccatcgcg gaaactctgg
     3061 agcgcatgga gtggatggcc aaagacgttt tcccgcaagt atagcaacag atcagtttct
     3121 gaaatggcaa cgcagcgttg aatgatgcga cgcgttatcc ccgatacaca ttaaggctcg
     3181 acggcagcac gcgattcaaa aactgtgaaa cgcacccttg cccgaatcta aaatccaaaa
     3241 tctaaaatct aaaatcgaaa acccctaagc cgccttgggt tggggaattt ccgccggcca
     3301 gttctcgata taaaccggct ccggcgggaa accgagcaca ttgaaatata aatattcccg
     3361 ccacgcagga aatttccgca cgttttccac cggaacattg aagtactttc gcagaaactc
     3421 gcgtcgcggt ggatcgaaga ccgtacgcgg ccggctgttc aaagcctctg catcgattgc
     3481 ggcgatgcgg cgccgaatcg gtgcttcgcc gtattgggcg atgagctccc atacgtagtc
     3541 gtggattttc tgtaaaatcg cgtcgtgatg gctacgctcc tcggcgtgta ccacgcgctc
     3601 ttcgaaatcg agcagcttcg gtgtggttat ctggatgctc tggatgctcg ctgcggtcac
     3661 gtggccgtag ggccacaaat tgccggaaaa aatgtaactc aaaaaatcgc gcttcatgat
     3721 ctgatggtgc tcgatccgcg gcgtaaggcc gtacttgcgc tcaaactcag ggtcgggttt
     3781 agcgtgatcg cggctcgggt cgatggcatt ggcctggcgg atgtaacccc agccgtggcc
     3841 tgcttcggtg ccggcatgcg cggcgagaaa gtaacgccat tcgcaatcca tttcaaagtg
     3901 cgccatcgcg taacacgccg tgccgactgt gatttcaagc tcgtcacggc cgcggtgagc
     3961 tagccagcgg gccaaacgtt cgcgctcctc gtcgtctttg ggtggccgtt tgatgttgtc
     4021 gcggcttttg cgcagtgcat cgacctccgc gctgatcgga ttatcttcgc tgtaggttat
     4081 tttttttccg acgcctcgaa tatccgccat atatcctcct ggtaagactt tttattttgc
     4141 tgcataaagc gagcgcacaa acccttcctt ttcgatgcgc tcgaggacgc tcatatccaa
     4201 taccccgtac gggttgcgat tggacgtcac aggcgaaagc ttgagaatcg tgtcgatggc
     4261 ctcccgcgac acgatcggaa accgctcgta gaccatcgtc acccgctgat aaacctcttc
     4321 gatcgcttcc gtgtcggcaa gccgcatggt tcgacttatg gttttctttg ccaggtctgg
     4381 atttgtgagc gcaaggttca cgccgtgaag aattccttta agcgatcgcg caaccaaaaa
     4441 aggttctttt tccagcattg cacgggtggt gacgactgaa gtaaacgggc aggggatacg
     4501 cgctgtcgcg agatccagaa tcatgtgtag cccggatttt ttcgcctcga tggtttccgg
     4561 tggtgataaa agcgccgcat cgacaactcc ggtgaacaaa gcttgcgaaa caagacccaa
     4621 tcgtccgaat gcgaccaact tcacatctct cgaaggatcg agccccgctc gcgctaacgt
     4681 cacctgattg gccaaattca gcgaagtccc tggaggggta ttggcgatga gcttccctct
     4741 caggtccttt acgctggaaa tctccggctt cacccaaagt tggaaaggaa tcgcgttcac
     4801 cagcccgctg atgatgcgaa cgtccacgcc tttcaagtac gcgctaagtg cttggccgcc
     4861 gccgacgatc gcaagttgca agctgccgga atcgagagcc cggatcatag tcgttccggc
     4921 cgactgcgta aattccacat tgacgccgta acgctcgaag agcttctgct cgacagcaat
     4981 agcgcccgcc aactgagtag cggttgcgcc ggaaaatcca aagcggagcg aatccgcgct
     5041 ccaaacaatc gcacggcagt ccgcgagtat gcagagcgcg aacccgaaca gacggattga
     5101 cctcgtcaaa caagaaaaac tattttgcac ttgcatgagg gttcgcgcag cgctcatcgt
     5161 tttcctttta tcacgcaaaa ccagcaaaac cacaaactgt cttgtagctg ttgcttaaga
     5221 ttaatgccct tcctgttgaa acatgccata cagttggact atctctttta agcacaagcc
     5281 ggcacatagc cggagaaccc tatcagccat gcgtcaccgg gtcatccacc tcatcaatcc
     5341 aaagacagac tcgcttacca cgcgtccgct ttatctcaat cgggcgctct actctccgct
     5401 cgcaggtttg ctcgccgtgg ccagtgccgt gccgcgagat cgttatgagg tggtattgac
     5461 cgacgagaac atagagccca tcgacttcga gctcgaggcg gacctcgtcg gcatctctgc
     5521 catgacatgt tacgtgaagc gcggctatga gatcgctgat gcattccgaa agcgcggagt
     5581 gccggtggtc atgggtggcg tccatccgag
//
LOCUS       DASULZ010000322         9922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_243658, whole genome shotgun sequence.
ACCESSION   DASULZ010000322 DASULZ010000000
VERSION     DASULZ010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9922
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243658"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VFY96_06680"
     CDS             <1..675
                     /locus_tag="VFY96_06680"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5606180.1"
                     /translation="VIAKAAEVRPIPRGLTTREAAAVPTTYLTAWFGLKTDGNLKAGE
                     WLLVQGGSSGVGTAAIQIAKHWNAKVIATTGSKEKALRLRELGADVTIDVSQHDFLPE
                     VMRATGGRGADVVLEMIGGEVYQKSLQALAPGGRLVSIGGAFGAIPEQPPALTEGRKA
                     TRFSITNYLKDKPEDFKQVDEILKLVNDRKFRVVIDKTFPLAETRAAQRYLEGREHFG
                     KVMLAM"
     gene            874..1929
                     /locus_tag="VFY96_06685"
     CDS             874..1929
                     /locus_tag="VFY96_06685"
                     /inference="COORDINATES: protein motif:HMM:NF027219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HEX5606181.1"
                     /translation="MAAEESTQKPLVAKTADVPPDIGSMSDEQLLQMRICDLKLKIPG
                     TELESRIEKFYAELAAKNIAVKPLCYLGDEWFCPEGSATIAIPFYLAHPRLKKLEEKM
                     MMEVEGGTESWCMRLLRHEMGHVLNHAYLLEKDKRWQKMFGPTSLEYSESFRARPYSR
                     RFVRHLEGYYAQSHPEEDFAETVAIWLTPDLDWRQQYRSWKALEKLEYVDDLMHKLAG
                     RPPVVLSKARISDASRLRSRLEAHYKRRRRIYAQEFPDFFDADLRKLFIDADAAPNGE
                     RASVFLRRSAKLILSAVSAWTGEPKFTINRLLRELTDRCAELDLRLKAPSAAVEIAAY
                     LATLACHYRLTGKFKDS"
     gene            1932..2960
                     /locus_tag="VFY96_06690"
     CDS             1932..2960
                     /locus_tag="VFY96_06690"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX5606182.1"
                     /translation="MVQGRKRHKVLVLFDTAGTPPANQDFTKELQTNDDWAAEAHVID
                     ALKKLGHDVRTIGVYDEPGMIIDEIKNNPPDLVFNLTEHFNAVSAYDRNVAGLLEMLG
                     VPYTGSSPTGLTLCKNKGMAKELLAYHKIRVPNFAVFSPGAAIKRPARLNFPLFIKPA
                     EEEASYGISLDSFVESDEAFEERVRFIHEKMNQTALVEEYIDGREIYVSLLGNDRLRV
                     FPFREVIFTQVPESQPKFSTFKAKWDDAYRKRWGIQNVFAEPFADGVHQRIVKICKAV
                     YRALRIRGYGRIDLRVTPEGEIVILEANPNPNLDRDDEFAQSADKAGLNYAHLIQRIV
                     MLGLTAAH"
     gene            2971..4227
                     /locus_tag="VFY96_06695"
     CDS             2971..4227
                     /locus_tag="VFY96_06695"
                     /inference="COORDINATES: protein motif:HMM:NF017263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEX5606183.1"
                     /translation="MSVLRPVRLERIWLRRAAPLLIVVFVVVLESRSMPAGLPRLVIL
                     NATDLIQTKERLQNRDPALAPAFTRLKREAERALDVKPLSVSEKKLSAPTGNKHDYVS
                     IAPYWWPNPATPNGLPFVHRDGKVNPTRDESSDRRRLENMIQTVQTLALGYFFTERED
                     YAAHAAELLRVWFLDPATKMNPHLKYAQGVPGRNTGRGAGIIETHEFADLMDFVSLLN
                     NSKAWPARQHQWRAWINVYLSWLLESAEGKAEAKAENNHGTWYDVQLAALALFTARDE
                     LAKKVLSEFPTKRVAAQIQPDGRQARELTRTRAWDYAIFNLEAMFNSASIAEKLNVDL
                     WNHPSANLGSLQRALDWLLPFGIGESKWEYSQITPFQPEKLAPLLRRASIAYGQPRYE
                     QAIAKLSALRGDERWRLLYAKLPDLK"
     gene            complement(4415..5620)
                     /locus_tag="VFY96_06700"
     CDS             complement(4415..5620)
                     /locus_tag="VFY96_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEX5606184.1"
                     /translation="MKFGIDRLLENSEIRKPLRNKQVGLLAHPASVTRELIHSLDALA
                     ALSDITLAAAFGPQHGLRGDKQDNMVESPDFHDPVHGIPVFSLYGDVRRPTAAMMDSF
                     DVLLVDLQDIGCRIYTFVTTLRYMLEAAAQHKKIVWILDRPNPVGRLVEGLRLRAGWD
                     SFVGAGALPMRPGLTLGELAKWFISTLNLDVECQIVELAGWKPEQPPGFGWPVGERAW
                     VNPSPNAANLSMARCYAGTVMFEGTRLSEGRGTTRPLELFGAPEIEPRHLLAKMNALE
                     PQWMRGCRLRECWFEPTFHKHAGKLCAGIQIHVDDSSYEHNRFRPWRLTALVLKALRA
                     LCPSYELWRNFTYEYERERLAIDLINGSPLLREWVDDPEATPADLENLAKLDERSWSE
                     EREPFLLYR"
     gene            complement(5617..6216)
                     /locus_tag="VFY96_06705"
     CDS             complement(5617..6216)
                     /locus_tag="VFY96_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HEX5606185.1"
                     /translation="MRAPKKTDAVLHCHPETLCSAVASLTTAITWLPGKALNFAYTVK
                     GAIDRLRLPPFESLRRGDSLWQRTCFEAFIGAKNDAEYYEFNFSPSGEWAAYSFRAYR
                     DGSAYTSDGFEPKIVAERGEDIFELSAAIDLDRFPELRGEVQLCVGISTVIETLDGSL
                     SYWALRHPPGKPDFHHSESFALELEPAVRDASARDPSHA"
     gene            6401..7123
                     /locus_tag="VFY96_06710"
     CDS             6401..7123
                     /locus_tag="VFY96_06710"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX5606186.1"
                     /translation="MALSGSETPAFDSEDLRELKQKVVDGCHILDREGITDGFGHVSI
                     RVPGTEAFLTIAAVSPGCATLERLVMLDLHGRYLGGQNAPPYEWPIHACIFKARPDVM
                     SVCHTHSRWSSLFSILRGGLPPIHMYAKFLPPGGPPIYPAAGLIGTVERGEALVRTLQ
                     DSAAVLLRAHGDAVVGASLEQAILRTLQLAFLGELAHMAVAHGEPLYLSQEELATFAA
                     DRSFPARPWEYYLTRIRERRSA"
     gene            7188..7904
                     /locus_tag="VFY96_06715"
     CDS             7188..7904
                     /locus_tag="VFY96_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX5606187.1"
                     /translation="MAGNRQDVPKIDSSELKELTEKLITACHILDHEGVTDGYGHISV
                     RVPGTDAFVTIANVSPGCVTRDRLIMQDLDGNYLDGAKTPPNEWPIHSCILKARPEIV
                     SVAHTHSKWSTIFSVLPIKLKPMHHYGKFLSADGPPVYEGVGLVRTMERGNELAAALG
                     KNPAVLMRAHGDAVVGESVEQVIERTTRLAMLGEWNHLALLHGAPKYLNAEELKVYNE
                     DQRFPMRGWEYYVSRLEGRK"
     gene            8008..8688
                     /locus_tag="VFY96_06720"
     CDS             8008..8688
                     /locus_tag="VFY96_06720"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX5606188.1"
                     /translation="MTSKEFVTQLKKDIQAFPKIRIKHPFLKAVCNGTATMDQIRTWT
                     IQDYQFRAAVPRIAMLRYLACNDPEIAQKLWGVVEEETRGMDTGTAGHNELAIRFAES
                     IGVTRSQLENAELLPSTAAHLYYVELIIHTLPWFVVMSIQIGAEGTFGPAAAALGNGF
                     IQKYKMKPEDVRFFTVHSEADEEHGSLAEEIAERYLHAPHLQELTRKHTFRRMELLYD
                     IWSVAGYY"
     gene            8740..9243
                     /locus_tag="VFY96_06725"
     CDS             8740..9243
                     /locus_tag="VFY96_06725"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX5606189.1"
                     /translation="MGNPAGVKRDFERLEQRRFRAYTLLKEGLSEAEVAREVGVHRQS
                     VNRWAQEIEKNGRRGLKKAGRAGRKPRLSEADLKKLETKLKEGPQPLGYETNLWTLER
                     IAKLIKQEFGVEYHPGHVGWLLGKLGWSCQRPTGRALERDEAAIKRWKKQIWPKLKKK
                     PSKKDVR"
     gene            9264..9749
                     /locus_tag="VFY96_06730"
     CDS             9264..9749
                     /locus_tag="VFY96_06730"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX5606190.1"
                     /translation="MSQRPHRCRTWSPKGETPVLQYSFNWKTLSAVAGISWWKFYFQL
                     HQGSIRSPQVVEFLRHLKRHIKRKLLVIWDRSKPHRSRLVKEFVAGQRGAICLEYLPA
                     YAPELNPVEYIWGHWKHHELPNFCPKTFTELSYHAIRALKRMRRRPKLITAFWKQAEL
                     F"
BASE COUNT         2349 a         2732 c         2731 g         2110 t
ORIGIN      
        1 gtgatcgcaa aagctgcgga agtccggccg attccgcgcg gcctgacgac gagggaggct
       61 gctgctgtac ccaccaccta tttgactgct tggttcggtc tcaaaaccga tggcaatctc
      121 aaagccggtg aatggctgct cgttcagggc ggtagcagcg gcgtcggtac ggcggccatt
      181 caaatcgcaa aacactggaa tgcaaaagtg atcgcaacca ccggcagcaa agaaaaagcg
      241 ctccggctcc gggagctagg cgccgatgta acgatcgatg tttcgcaaca cgattttctg
      301 cccgaggtca tgcgcgcaac cggcgggcgc ggtgcggacg tcgtgctcga gatgatcggc
      361 ggcgaggtct atcaaaagag cttgcaggca ctcgcgccgg gaggtcggct ggtttccatc
      421 ggcggcgcat tcggagcgat tccggagcaa ccgccggcat tgactgaagg gcgaaaagcg
      481 acacgattct cgatcacgaa ttacttgaag gacaagcccg aggatttcaa acaagttgac
      541 gagattctaa agctcgtcaa cgatagaaag tttagagtgg tgatcgataa gacattcccg
      601 ctggcggaaa ctcgcgccgc gcagcgctat ctcgaaggcc gcgaacactt cggcaaagtg
      661 atgctcgcga tgtaggcttg caaacagaag ggtttctcgt cggttcgctc cgtgaaatga
      721 caaagcgttt gaggcctgtc atctcgactc ccagcaaaaa ttcgacgccg ttgcctctga
      781 cgtctacttc agcttcaaac cagcggatcc ttcaaccaat cattccaata ctccatcact
      841 ccagcactcc attactcctt tgacgcagac tccatggctg cagaagaatc cacccaaaaa
      901 ccactggtag ccaaaacggc agatgtgccg ccagatatcg gcagcatgag cgacgaacaa
      961 ctgttgcaga tgcgcatctg tgatttaaag ctcaaaatcc cgggcacaga gctcgagtcg
     1021 cgcatcgaga aattctacgc ggaactcgcc gcaaaaaata ttgcggtcaa accgctgtgc
     1081 tatctcggcg acgagtggtt ttgtcctgaa gggagcgcca ccatcgctat tccgttttat
     1141 cttgcgcacc cgcgactaaa aaaactcgaa gaaaagatga tgatggaagt cgagggcggc
     1201 accgagtctt ggtgcatgcg actgctgcgt catgagatgg ggcacgttct caaccacgct
     1261 tacctgctcg agaaagataa acgctggcaa aagatgtttg gacccacttc actcgaatat
     1321 tcggaaagtt ttcgcgctcg accctatagc cggcgattcg tgcgtcactt ggaaggctat
     1381 tacgcgcaga gccatccgga ggaggatttc gccgagaccg ttgcgatttg gctgactccc
     1441 gatctcgact ggcgccagca gtatcgcagt tggaaagctt tggagaaact cgaatatgtc
     1501 gatgacctga tgcacaagct tgccggcaga ccgccggtgg ttttatccaa ggctcggatc
     1561 agcgatgcgt ctcgtcttcg ctcccgactg gaagctcatt acaaacggcg ccggcgaatt
     1621 tatgctcagg agtttcctga ttttttcgat gccgatctca gaaaactttt catcgacgcc
     1681 gacgcagcgc ccaacgggga gcgcgcttcg gtgtttttgc ggcgttcggc caagctgatt
     1741 ctgagcgcgg tctctgcctg gaccggcgag ccaaaattta cgatcaatcg tttgctgcga
     1801 gaattgaccg atcgctgcgc cgagctcgat ctgcggctga aagcgccgtc ggcggccgtg
     1861 gaaattgccg cctatctggc aacactcgcc tgccactacc ggctcacggg caaatttaaa
     1921 gactcttgat catggtccaa gggcgcaagc ggcacaaagt cctggtgttg ttcgataccg
     1981 ccggcacgcc accggccaat caggatttca ccaaggaatt gcaaaccaac gatgactggg
     2041 ccgcagaagc ccatgtcatc gatgctctaa aaaaactcgg ccatgacgtc cgaaccatcg
     2101 gtgtttacga cgagcccggc atgatcatcg atgaaatcaa gaacaatccg cccgacctag
     2161 ttttcaatct caccgaacat ttcaacgccg tttcggctta tgatcggaac gtcgccggtc
     2221 tgctggagat gctgggcgtc ccctacacgg gctcgagccc caccggactg accctttgca
     2281 aaaataaagg catggccaag gagcttctcg cctaccacaa aattcgcgtg cccaacttcg
     2341 ccgtgttctc tcccggagcg gcgatcaagc gtcccgcccg gctaaatttt ccacttttta
     2401 tcaagccggc tgaagaagag gcgtcctatg ggatttctct cgattctttt gtcgagtctg
     2461 acgaggcctt cgaggagcgg gttcgtttca ttcatgagaa gatgaatcag actgcgctcg
     2521 tcgaggaata catcgacggc agggaaattt acgtgagcct tctcggtaac gaccgattac
     2581 gagtatttcc tttccgtgaa gtcattttta cgcaagttcc cgagagccag ccgaaattct
     2641 cgacgttcaa agccaaatgg gatgacgctt accgcaagcg gtggggaatt cagaatgtct
     2701 tcgccgagcc gttcgcagac ggcgttcacc agcgcatcgt caagatctgc aaagccgtgt
     2761 accgagctct gcgcattcgc ggctacggcc gtatcgacct tcgcgtgacg ccggaaggcg
     2821 agatcgttat ccttgaagcc aatcctaacc cgaatctcga tcgcgacgat gaatttgccc
     2881 aatccgcaga caaagcaggc ctgaactatg ctcatttaat tcagcgcatt gtcatgcttg
     2941 gcttgaccgc tgcgcactag gcggaacgtc atgtctgtct tacgacccgt tcgactcgag
     3001 cgaatttggc tccgacgggc agctcctttg ctcatcgtcg tcttcgttgt cgtgctcgag
     3061 agtcgctcga tgccggctgg attaccgcgt ctggtgattt tgaatgcgac cgacttgatc
     3121 cagacaaaag aacggctaca gaatcgtgat ccggctcttg ccccggcatt taccagactt
     3181 aagcgcgagg ccgagcgggc gctcgatgta aagcctctgt ctgtaagcga aaaaaaactc
     3241 tcggcgccca cgggcaacaa acatgattac gtgagcatcg ccccatattg gtggccgaat
     3301 cccgcgacgc cgaacggact tccttttgtc caccgcgacg gtaaagtgaa tcccacgcgt
     3361 gatgagagct ccgatcgccg gcggctcgaa aacatgatcc agaccgtgca gacccttgcg
     3421 ctcggctatt tttttaccga gcgtgaagat tatgcggccc atgcagcaga acttttgcgg
     3481 gtctggtttt tagatccagc gaccaagatg aacccgcacc tgaaatatgc gcaaggcgtc
     3541 cccgggcgaa ataccggccg gggcgcgggt atcattgaaa cccatgagtt tgcggacctt
     3601 atggatttcg tctcgctgct caacaattcc aaggcatggc cggcgcgcca acatcagtgg
     3661 cgagcttgga ttaacgtata tctcagctgg ctcctcgaaa gcgcggaagg caaggcggaa
     3721 gcgaaagccg agaacaatca cggcacatgg tacgacgttc agctggccgc cctcgcgctc
     3781 ttcacagcac gcgacgagct cgcgaaaaag gttctgagcg aatttccgac taagcgcgtc
     3841 gccgcacaga tccaaccgga tggccgtcaa gcgcgagagc tcacgcgcac ccgtgcatgg
     3901 gattatgcga ttttcaatct cgaggctatg tttaattccg catcgatagc ggagaagtta
     3961 aacgtggacc tatggaatca cccgagcgcc aacctaggca gtctccagcg agccttggat
     4021 tggctgctgc cgttcggtat cggtgaaagt aaatgggagt attcgcaaat tacgccgttt
     4081 cagccggaga agcttgctcc gctcctgcgc cgcgcgtcaa ttgcatacgg ccagccacgg
     4141 tacgagcagg ccatcgcaaa gttaagcgca ctgagaggcg atgaacgctg gcgattgctc
     4201 tatgcgaaac tgccggatct caaatagtca tctactgggc aagaaccccg gctttcgcta
     4261 cgcttcatcc gggctacaca ggagacgctg tgttgcgatc atcccggtgt catttcgacc
     4321 ccgttcgagc tcagggtaaa ctccgggaga aatcgttctg aaatccctgc tcgagttagc
     4381 ttgcgcccaa gttttgagat cactcttgga ttgactaccg atataacaaa aacggttccc
     4441 gctcttcgct ccacgagcgc tcgtcgagct tcgcgagatt ctccagatca gcaggagtag
     4501 cctcaggatc atcgacccat tctcgaagca aagggctgcc attgatcaag tcgatggcaa
     4561 gacgctctcg ttcgtattca taggtaaagt tgcgccagag ttcgtagctg ggacaaagcg
     4621 cgcgtaacgc tttcaatacg agggcggtca ggcgccacgg ccgaaacctg ttatgctcgt
     4681 aactcgaatc atcgacatga atctgaatgc cggcgcatag tttgcctgca tgtttgtgaa
     4741 atgtcggctc gaaccagcac tcgcgcagac gacagccgcg catccactga ggctcaagcg
     4801 cattcatttt tgcgagcagg tggcgtggct cgatctccgg ggcgccaaat agttccagcg
     4861 gccgggttgt accccgccct tccgatagac gggtgccttc gaacatgacg gttccggcgt
     4921 agcagcgcgc catcgacaaa ttggccgcgt tcgggcttgg attgacccaa gcgcgttcac
     4981 ccacgggcca gccgaatcca ggcggctgct ccggtttcca acctgccagc tcgacgattt
     5041 ggcactccac atctaggttc agcgtgctta tgaaccattt tgccagctcg cccaaagtca
     5101 gaccagggcg catcggcaaa gctccagcgc cgacaaaact gtcccaccca gcgcgtaacc
     5161 gaaggccttc gactagccgg cccaccgggt tgggccgatc gagtatccag actatctttt
     5221 tatgttgagc tgctgcttcg agcatataac gaagtgttgt aacgaaggtg taaatccggc
     5281 agccgatatc ctgaagatcg acaagcagca cgtcgaagct gtccatcatt gccgcggtgg
     5341 gccggcgcac atcgccgtac aaactaaaaa ccggaatccc gtgcaccgga tcatgaaaat
     5401 ccggcgattc gaccatattg tcctgcttat cgccgcggag cccgtgctgc ggaccaaagg
     5461 cagcggctaa cgtgatgtcg gaaagcgctg ctaaagcatc gagggaatga attaattcgc
     5521 gcgtgaccga cgcgggatgg gcaagcaagc cgacctgctt atttcgcaat ggcttgcgaa
     5581 tctccgaatt ttcgagaagc cgatcgatgc caaacttcat gcgtggctcg gatcgcgcgc
     5641 gctggcgtcc cggaccgcgg gttcgagctc cagtgcgaaa ctttccgagt gatgaaagtc
     5701 gggcttgccc ggcggatgtc tgagcgccca ataggaaagc gatccgtcga gagtttcgat
     5761 caccgtcgaa attccgacac acaattgaac ttcgcccctg agttcgggaa agcgatccaa
     5821 gtctatggcc gcgctaagct cgaaaatatc ttcgccccgc tcggcaacga ttttcggttc
     5881 gaaaccgtcg ctcgtataag ccgaaccatc gcgataagct cgaaacgagt aggctgccca
     5941 ttctcccgaa ggagaaaaat tgaattcgta atactcggcg tcgttttttg cgccgataaa
     6001 cgcttcaaaa caagtccgct gccataaaga atccccgcgc cgaagtgact caaacggcgg
     6061 aagcctaagc cgatctatgg cgcctttgac cgtataggcg aaattaaggg ctttcccggg
     6121 cagccaggta atggctgtgg taaggctcgc gacggcggag cacaaagtct cgggatggca
     6181 gtgaaggacg gcgtcggttt ttttcggtgc tcgcatgaaa tccgatgaaa aaaactcccg
     6241 tggctttcag aaatttagag agtaacactc caatgatcag tcgagtgtgg attgttatcg
     6301 gtattaaccg cgcagcatga aactgtcaag tcaattctca gcttgacaca tcggcaccgc
     6361 gaacttacaa cctcagaagt tcatgaatca ggagaaataa atggcgttat ccggctcaga
     6421 aactccggcc ttcgactcgg aagatcttcg agagctcaag caaaaagtgg ttgacggctg
     6481 ccatattctc gaccgcgaag gcatcaccga cggattcggg cacgtcagca tacgtgttcc
     6541 cggaaccgaa gcgtttttaa cgatcgcggc agtgagcccc gggtgcgcca cactggagcg
     6601 gctcgtaatg ctcgatctcc atggccgata tcttggtggc cagaatgctc cgccgtacga
     6661 atggccgatc cacgcctgca tcttcaaggc gcgtcccgac gtgatgagcg tctgtcacac
     6721 ccactccaga tggagctcgc tttttagtat tttgcgcggc ggcctgccac cgattcacat
     6781 gtatgcgaag tttctgcctc ccggcggccc accgatctat ccggcggccg ggctcatcgg
     6841 tactgtcgag cgcggtgaag ctctggttcg gactctccag gacagcgcgg cagttttgct
     6901 tcgcgcccac ggtgatgctg tggtcggagc gagtctcgag caagcgattt tgcgcacgct
     6961 tcaacttgcc tttctgggcg agctcgcaca catggccgtt gctcatggcg agccgctcta
     7021 tctgagccaa gaggagttgg cgaccttcgc cgccgatcgg tcatttcccg cgcgcccgtg
     7081 ggaatattat cttactcgta tccgcgagcg acgctctgct tgacagtccc aatatcgacg
     7141 catagattcg ccgaacagat agactgattt tccatcgagg ggtagtcatg gctggcaatc
     7201 gtcaagacgt gccgaagatc gattcttccg aattaaaaga gttgaccgaa aaacttatta
     7261 ccgcatgcca cattttggat catgaaggcg tcaccgacgg atacggtcac atcagcgtgc
     7321 gcgtcccggg cacagatgct ttcgttacca tcgccaatgt cagcccgggc tgtgtcactc
     7381 gcgatcgact gatcatgcaa gatctcgacg ggaattattt ggacggcgct aaaacaccgc
     7441 ccaacgagtg gccgatccat tcctgtattc ttaaagcgcg gccggaaatc gtcagcgtgg
     7501 cccatactca ttcgaaatgg agcacgatct tcagcgtttt gcccatcaag ctcaaaccga
     7561 tgcaccacta cggaaaattt ctctccgcgg acggtccacc ggtctatgag ggcgtgggtt
     7621 tggtgcgcac gatggaacga ggcaacgaat tggctgccgc acttggcaaa aatccggcgg
     7681 ttttgatgcg tgcccacggt gacgctgtcg tcggagaaag cgtcgaacag gtgatcgagc
     7741 ggactacccg cctcgccatg ctcggcgagt ggaaccatct cgcactttta cacggcgcac
     7801 caaaatatct aaatgcagaa gagctgaagg tttacaacga ggatcagcgc tttccgatgc
     7861 gcggctggga gtattacgtg agccggctcg agggacggaa ataactggag taacggagtg
     7921 gcggagtgat gggttcgaca atactccagt agtcgaacac tccattactc ccggaaaacg
     7981 aggcagagaa accaggagga aagtcatatg acaagcaaag agtttgtcac ccaactcaag
     8041 aaggacatcc aggcgtttcc caaaatccgc atcaagcatc cgttcttgaa agcggtatgc
     8101 aacggcaccg caaccatgga tcagattcgc acctggacga ttcaggatta ccaatttcgc
     8161 gctgccgtgc cgcgcattgc gatgctgcgc tatttggcct gcaacgatcc tgagatcgcg
     8221 cagaagctct ggggtgtcgt cgaagaagaa acccgtggca tggataccgg cactgccggt
     8281 cataacgagc tcgcgattcg cttcgcggaa tcgatcggcg tcacacggag ccagcttgaa
     8341 aacgccgagc tcctgccgtc aaccgcggct catctttatt atgtggaatt gatcatccat
     8401 acgctgccat ggtttgttgt catgtcgatt caaatcggcg ccgaaggcac tttcggtccg
     8461 gccgcggccg cgctcgggaa cggatttatt cagaaataca agatgaagcc cgaggatgtg
     8521 cggtttttca ccgttcactc ggaagcggac gaagaacatg gcagcctcgc cgaagaaatc
     8581 gccgagcgtt atctccatgc gccgcatctg caggagctca cccgaaagca tacttttcgt
     8641 cgcatggaac tgctctacga tatctggtcg gtggctggtt attattgagg tgcataaaat
     8701 atagtgactt aggacggcac gtatggtaaa tagctccgca tgggaaatcc agcaggagtc
     8761 aaaagggact ttgagcgatt agagcaaaga cgctttcggg catacacact gttgaaagaa
     8821 ggcctgagcg aagccgaagt tgcccgagaa gtgggcgtgc atcgccagtc ggtcaatcgc
     8881 tgggcgcaag agatcgagaa gaacggccgg cgcggcttga aaaaagcggg ccgtgcgggg
     8941 cgcaagccac gtctgagtga agcggattta aaaaagctcg agaccaagct caaagaaggg
     9001 ccgcagccgc tcggatacga aacgaacctg tggactctcg aacgtatcgc gaagttgatc
     9061 aagcaggagt ttggagttga gtaccacccc ggccacgtgg gttggctctt aggaaaactg
     9121 ggatggagtt gccaacggcc tacgggccgg gcgttggagc gcgatgaagc agcgatcaag
     9181 cgatggaaga aacagatttg gccgaagctt aaaaaaaagc ccagcaagaa agacgtacga
     9241 tagtgttcat tgatgaaagt ggattgagcc aacgaccgca tcgctgtcgg acgtggtcgc
     9301 caaagggcga gaccccggta ttgcagtatt cgtttaactg gaagacgctc tcggcggtag
     9361 cgggaatttc ttggtggaaa ttttactttc agctgcatca ggggagcatc cgcagtcctc
     9421 aagtcgtgga gtttctcaga caccttaaac gtcacatcaa acgcaaactg ttggtcatct
     9481 gggatcgttc taaaccgcac cgcagccgtt tggtgaaaga gtttgtggcc ggccaaaggg
     9541 gagcgatctg cctggagtat ctgccggcct acgcgcctga gcttaaccca gtggagtaca
     9601 tctgggggca ctggaaacac catgagctgc cgaacttttg tcccaagacg tttaccgagc
     9661 ttagctatca tgccattcgg gctctcaaac ggatgcgtcg tcggccaaag ctgatcactg
     9721 cattttggaa acaggctgag ttgttttgaa atcagttttg tcactatatt ttacgatcat
     9781 caataatagc ggtgtcaaag tcgacgaaaa tgcgccaggc agagtctggt tggatcagtc
     9841 gggtgaagtc tctcccgcgc cggtttacaa aaaagatgtc ggcacacgcg ccctcaccaa
     9901 actcaaggag cggtttctta gt
//
LOCUS       DASULZ010000323         3260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_954735, whole genome shotgun sequence.
ACCESSION   DASULZ010000323 DASULZ010000000
VERSION     DASULZ010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954735"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            305..1363
                     /locus_tag="VFY96_06735"
     CDS             305..1363
                     /locus_tag="VFY96_06735"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEX5606191.1"
                     /translation="MLLCLVFVCSGFTYQLALPGRKITFPADHYSHPNFKTEWWYYTG
                     HLESESGRRYGYQVTFFRFGLRDRQNEVKEKPPFSELYMAHFAVSDVNAKEFRYRERI
                     NRGYNGKAGAATDRYFVWNEDWKVEGDRNNHKIQVSDRGTELQLNLKSLKPPVLHGSN
                     GLSQKAAGEGRASYYYSLTRMQSDGALTIDGKTERVRGMSWMDHEFGSNQLGQDQVGW
                     DWFSIQLDDQTELMLYLMRRKDGTVDPYSSGTLVQADRSTRHLKLSDYRVEVLGRWRS
                     PTSGADYPMKWRVTIPAEQIELEINPAFQNQELITNRSTRVTYWEGAVDVRGRASGKT
                     VSGAGYVEMTGYAGKLSF"
     gene            1367..2806
                     /gene="tilS"
                     /locus_tag="VFY96_06740"
     CDS             1367..2806
                     /gene="tilS"
                     /locus_tag="VFY96_06740"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX5606192.1"
                     /translation="MSELRDIFKHYAMKHSLLCSGDRVLVAISGGPDSVSLLHLLYEL
                     KDKWDLHLEVAHLQHGIRGDTAQQDAIFVERLAEELKLPFHMREVDLPRLRSAAGRGN
                     LEALARAERYRFFAELVASRKLSKVATAHTQDDQAETVLMWFLRGAGLRGLGGMAPLQ
                     QIHIASEKSLSLTVVRPLLDISKAELLHYLSTRSIAYCIDESNQDRAYLRNWIRLELL
                     PKIRARAGTGISARLTQQAGLIRDEDALLDSLTVQTYGSVSDNGDLLRCALLKQPKAL
                     QRRILRYWIEQARGHLRGIDFIHIEALVRLIDEGPAQGKLSIPAGWEFVREYDRLRLG
                     KSSRGTHRVCYSYELKIGTPLRICEAGFELNAELIAASGARLPADLREALFDAACLTE
                     PLLVRNFRHGDRFKPLGVPGHKKLKDLFIENKIPLSIRATLPLVVMGQEILWIPGYGR
                     SETALVGEKTERVVHIKSFVYRDLTHDTL"
     gene            2866..>3260
                     /locus_tag="VFY96_06745"
     CDS             2866..>3260
                     /locus_tag="VFY96_06745"
                     /inference="COORDINATES: protein motif:HMM:NF018218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HEX5606193.1"
                     /translation="MSQSSKHIALWLVLALIFLAIFSVFNKQHGREPEIVFSEFMTSV
                     DRGDVQKVTIQGHNIQGEYKNGERFRTFAPNDPELVKSLREKKVKIAAKPEEDSPWYM
                     VLLLNWFPMLLLIGVWIFFMRQMQVGGGKA"
BASE COUNT          756 a          794 c          923 g          787 t
ORIGIN      
        1 cgcacatcga tgatgcatac atcgggggcg ccgagcttgt gccgcaaatc atcgactgtt
       61 gtgagcagag tttcgcgcgt atattcttgc ttcataccga tccgtataac ctagtcaaaa
      121 actcactgca atcatgcgat tatttgttgg ccgcccaaac ttctgttcat tagccgaacg
      181 ccagtttgtc accccgagcg gatgcggggg atcttttccg cagaaagatt tctccgcttc
      241 ggctcgaact ggcagtggac gacaggctca agggattttc cgacgacccg cttacttggt
      301 tatcgtgctc ctttgcttgg tcttcgtttg ctccggcttc acatatcagc tggctttgcc
      361 cggccgcaag atcacttttc ccgccgatca ttactcccat ccaaacttta aaacggaatg
      421 gtggtattac accggacatc tcgaaagcga aagcggcagg cgctacggtt accaagtcac
      481 tttttttcgc ttcggcctgc gcgatcggca gaacgaggtc aaggagaagc cgccctttag
      541 cgaattgtac atggcgcact tcgcggtttc cgatgtcaat gcaaaggaat ttcgttatcg
      601 cgaaagaatc aatcgcggtt ataacggcaa agccggcgct gctaccgatc gttatttcgt
      661 ttggaacgag gattggaagg tcgaaggcga ccgcaataat cataaaatcc aagtgagcga
      721 ccgcggcacc gaattgcagc tcaatttaaa atcgctcaag ccgccagtgt tgcacggttc
      781 gaacggtctc agccaaaaag ccgccgggga agggcgtgca tcgtattact actctttaac
      841 ccgcatgcaa agcgacggcg cactgaccat cgatggtaag acggagaggg tccgcggtat
      901 gagctggatg gaccacgaat ttggcagcaa tcagctgggg caagatcaag tcgggtggga
      961 ttggttcagc attcaactcg acgaccagac cgagctcatg ctttacctga tgcgtcgcaa
     1021 ggacggaact gttgatccct attcgagtgg tactttagtg caggccgacc gttccactcg
     1081 gcatctcaag ctgagtgatt atcgggtcga ggtgttaggg cgctggcgaa gtccgacgag
     1141 cggggcggat tatccgatga aatggagagt gacgatcccc gcggaacaga tcgagctcga
     1201 aatcaatccg gcatttcaga atcaggagtt gatcaccaat cgcagcaccc gggtgactta
     1261 ttgggagggg gccgtcgacg ttcgtggaag agccagcgga aagactgtca gcggcgccgg
     1321 ctatgtcgag atgaccggat acgcggggaa gttaagcttc tgaaccatga gtgagctgcg
     1381 cgacatcttc aagcattatg caatgaagca cagtctgctg tgctcgggtg atcgcgttct
     1441 cgtggcgatt tccggtggtc cggattccgt ttcattgttg catcttctct acgaactcaa
     1501 agacaaatgg gatctgcatc tcgaagtggc ccatctgcag catgggattc gcggcgacac
     1561 cgcgcaacaa gatgcaatat ttgtcgagag attggcggag gaactgaaac ttccatttca
     1621 catgcgagag gtggatctgc cgcgcctgag gtcggcggcg ggaaggggaa atcttgaagc
     1681 gttggcgcgg gcggagcgct atcggttttt cgccgagcta gtcgcttcaa gaaagctttc
     1741 caaagttgcg accgcgcata cacaggatga ccaggctgag acggttctga tgtggtttct
     1801 tcggggggcc gggctgagag ggctgggtgg gatggcgccg cttcagcaga ttcacattgc
     1861 gtccgagaag tcgttatcgc taactgtcgt acgcccgctg ctggatatct ccaaggctga
     1921 acttttgcat tatctgagca caagaagcat cgcctactgc atcgatgaga gcaatcagga
     1981 tagggcgtac ctccgcaact ggattcggct ggagctcttg ccgaaaattc gtgcgcgtgc
     2041 tggtactgga atctcagctc gcctgactca gcaagccggt ctgattcgtg atgaagatgc
     2101 attgttagat agtttaacag tacagaccta tggctctgtt tccgataatg gagatttgct
     2161 tcgctgcgct ctgctgaagc agcccaaagc actccaaaga cgaattctgc gttactggat
     2221 cgagcaagcg cgcggtcatc tccgcgggat tgatttcatt cacatcgaag ccttagtgcg
     2281 tctaatcgat gagggtcccg cgcagggaaa actttcaatt cccgccggat gggagtttgt
     2341 tcgcgagtat gaccggttgc ggttggggaa aagttcgcgc ggaacgcacc gggtctgcta
     2401 cagctatgag cttaaaatcg gcacgccgct ccggatttgt gaagctgggt tcgaattgaa
     2461 cgctgagctc atagcagcca gcggtgccag gttgcctgcc gatctgaggg aagcgctttt
     2521 tgatgcggct tgcttgacag agccgttatt ggtgcgaaac tttcgtcacg gtgaccgttt
     2581 caaacctttg ggtgtgcctg gacataagaa gctcaaggat ttgttcatcg agaacaagat
     2641 tccattgtca attagagcga cattgccact tgtcgtcatg ggacaagaaa ttctttggat
     2701 tcctggttat ggcagaagcg aaacggcgct tgtgggcgag aaaaccgagc gcgttgtgca
     2761 cataaaaagc tttgtctatc gagacttaac acatgatacg ttatgattga tcgaagtaat
     2821 cgcgtgtgtt acactttttt tcgtttctaa aacctggagg aacggttgag tcagtcatca
     2881 aaacacattg cgctatggtt ggtcctggcg ctgatttttc tcgcgatatt tagcgttttc
     2941 aataaacagc acggccgcga gccggagatt gttttcagcg agttcatgac ttcggtcgat
     3001 cgcggagatg tgcaaaaagt cacgattcag ggccataata tccagggaga atacaaaaac
     3061 ggcgagcgtt ttcgcacctt cgcgcccaac gatccggagt tggtcaaatc gttgcgcgaa
     3121 aagaaggtta agatcgccgc caaacccgaa gaagattcgc catggtacat ggtgctattg
     3181 ctcaactggt tccccatgct tttgttgatc ggcgtgtgga ttttcttcat gcgccagatg
     3241 caagttggcg gcggcaaagc
//
LOCUS       DASULZ010000324         4833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_954762, whole genome shotgun sequence.
ACCESSION   DASULZ010000324 DASULZ010000000
VERSION     DASULZ010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954762"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..828)
                     /locus_tag="VFY96_06750"
     CDS             complement(136..828)
                     /locus_tag="VFY96_06750"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX5606194.1"
                     /translation="MIIGHRSSRAEKKFRSLTKLLFQKLKDAIVSGKLEPGERLSETK
                     LAASLKVGAFAVREALRKLEAQGYVTFQTDNQPIVSKPTREEIEDHYAIAGVLEGLAA
                     RLAVERAHPEEVEHLTQLHHSLKEASQKRDLTRYFEANNNFHRAIADMARNERLYRLI
                     DELRQTMQKTRILALRLPERLDYSMREHDQILDAFLKKNSELAETAMVRHLTNHMKTL
                     HKALDISKGEKK"
     gene            complement(840..2333)
                     /locus_tag="VFY96_06755"
     CDS             complement(840..2333)
                     /locus_tag="VFY96_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX5606195.1"
                     /translation="MQSVYVAGVGLTRFGKDPRALGEIFSEAAKRALGETEEVDALYI
                     GVMNPEEFTGDSNIASLIADAVGLTGVPATRVETASSAGAAALMAGFQAIASGYYRRV
                     LVLAGEKMTHLSTSATTRVLAEVIDPRERLCGATMPALAAMITEKYRQKFHLSEARLQ
                     NMLCSIALKNHGNGVKNPSAQFQRAISRETYFGSKWVATPLRLYDCAPITDGAAAVVL
                     TREPSDVRLSGVGQGTGVLSLQDREVFTSFPSTQIAAARAYAMANTEPKGIDFAEVHD
                     AFTPFEIISTEDLGFFPPGKGGDAAVEGKTTIDGAMPINLSGGLKARGHPVGASGLAQ
                     VVEVTKTLRGESPWGRAFKRGLTQSTGGLGTNNFVTILERAGAPTVQNPWKGATALAN
                     KPARTAPRARLADEGVIETFTIVHVTPDGFLPPLALALVRTMDGRLIMAQGEENDPAL
                     KIGREVYLRRIDGLYLFTVKSHIQRVQEAFTKLLRRKVAAELSQSSK"
     gene            complement(2544..3173)
                     /locus_tag="VFY96_06760"
     CDS             complement(2544..3173)
                     /locus_tag="VFY96_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-tRNA-protein transferase"
                     /protein_id="HEX5606196.1"
                     /translation="MDRVWAEGWRHFGIVFMRYPKAVHRGREFTVLPLRIDLARFRPT
                     RSQKRVLAKNRDTRVIVRSSFVDSEKEALFEKHRLRFDENVPTSLFNFLSTIPGSVPC
                     VNVELCVYLGARLSGVTFLDVGAQATSAVYAMFDPAELKRSLGIFMMLRSIEFSLRRG
                     CCYYYPGYAYREPFAYDYKKRFIGLEAFDWADGWKPFHRDDATGIIKPR"
     gene            complement(3425..3664)
                     /locus_tag="VFY96_06765"
     CDS             complement(3425..3664)
                     /locus_tag="VFY96_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606197.1"
                     /translation="MSAPTSLLMLEFLAWVASRHRTYGDVMNAWQSTCPRHTIWEDAM
                     IDGYVQVQRSETRQDPEVTLTPAGRALLNGTAAKL"
     gene            complement(3850..4326)
                     /locus_tag="VFY96_06770"
     CDS             complement(3850..4326)
                     /locus_tag="VFY96_06770"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5606198.1"
                     /translation="MTHSDLSLSLARRTDAPAIAKMSRALIEYGLQWKWTAQRVEASI
                     CAANVNVLVARLETRIIGFSIMRYGDDDAHLDLLAVAPPYRRLGVGQELLQWLEKCAT
                     VAGIFRITLEVRESNSGAQQFYERMGYHRLNLVPGYYQGTEAALRMRRDLASGSGC"
BASE COUNT         1110 a         1339 c         1294 g         1090 t
ORIGIN      
        1 cattatgatc gctccggccg cgcaacgtga actcgaaaag agctccgtag aaccctagcg
       61 cttggtccaa atcatcgact tccaaaacca cgtggtttac acccaaagcg cgggcttttg
      121 tttgctcacc attgttcatt tcttttctcc cttgctgata tcgagtgcct tatgaagagt
      181 tttcatgtgg tttgtcaaat ggcgcaccat cgcagtttcg gctagctctg aattcttctt
      241 tagaaaggcg tccaagatct ggtcgtgttc gcgcatcgaa taatcaagcc gttcgggcaa
      301 acggagtgcg agaatgcgcg tcttttgcat cgtttgccgc agctcatcga tcaatcggta
      361 gaggcgttca ttgcgcgcca tatcagcaat cgctcggtgg aagttattgt tagcttcgaa
      421 gtaacgtgtc agatcgcgtt tttggctggc ctctttcagc gaatgatgca gctgagtcaa
      481 gtgttccacc tcttcgggat gagcgcgctc aaccgccaat ctagcggcca agccttcgag
      541 cacgccggcg atcgcatagt ggtcttcaat ctcctcacgc gtcggtttac tgacaatcgg
      601 ctggttatcg gtttgaaagg taacgtaacc ttgggcttcg agcttgcgca acgcttcacg
      661 aacagcgaat gctcctacct tcagagaggc tgcgagttta gtttcagaca accgctcgcc
      721 tggctcgagc ttgccagaga caatggcatc cttcaatttt tgaaagagaa gctttgtcag
      781 tgatcggaat tttttttcgg ctcggctgga gcggtgccca atgatcactc ggggctctcc
      841 tacttgctcg attgagagag ttccgccgca actttacggc gcaataactt ggtgaaagcc
      901 tcctgcactc tttggatgtg actcttcact gtaaaaaggt acaggccgtc gattcgtctg
      961 agatagactt ctcggccgat tttcaacgcc gggtcgttct cctcgccttg agccattatc
     1021 agccggccgt ccattgtcct cacgagcgcg agcgccaggg gaggaagaaa gccgtccggt
     1081 gtcacatgta cgatcgtaaa ggtttcaata acgccctcgt ccgccaaacg ggcccgcgga
     1141 gccgtgcgcg cgggcttatt cgccaaagct gtggctcctt tccaaggatt ctgaaccgtc
     1201 ggcgcaccgg cgcgttcaag gatggtcacg aagttattgg tgcctaggcc gccggtgctt
     1261 tgcgtcaagc cccgtttgaa cgcgcgtccc catggactct cgccgcgaag ggttttagtg
     1321 acttccacta cctgcgccaa tccgctcgcg ccaacaggat ggccgcgcgc tttgagaccg
     1381 ccggagagat tgatcggcat agcgccgtcg atcgtggttt tcccctcgac cgcagcgtcg
     1441 ccgcccttcc cgggaggaaa gaatccgaga tcctcggtgc tgatgatttc aaagggagta
     1501 aaggcatcat gaacctcggc aaagtcgatt cccttcggtt ctgtgttcgc catcgcgtaa
     1561 gcgcgtgcgg ccgcgatctg cgtcgatggg aaagaggtga acacttcgcg atcctgaagg
     1621 ctcaaaactc ccgtgccttg gcccacgccc gagagacgca cgtcgctcgg ttcacgagta
     1681 agcacaacgg cggccgcccc gtcggttatc ggcgcgcaat catagagccg caacggcgtc
     1741 gcgacccatt tgctgccgaa gtaagtctcg cgggagatcg cgcgctgaaa ctgcgccgac
     1801 gggtttttga cgccattgcc gtggttttta agcgcaatgc tacaaagcat attttgcagg
     1861 cgcgcttccg acaggtgaaa cttttgtcga tatttttccg taatcatcgc cgccagtgcc
     1921 ggcatggtcg cgccgcacag gcgctcccgc ggatcgatga cttccgccag aacgcgcgtc
     1981 gttgcgctgg tgctgagatg ggtcatcttt tcgccggcca gaactaggac acgacgatag
     2041 tacccagagg cgatcgcttg aaatcccgcc atgagcgccg cggcgcctgc ggaagacgca
     2101 gtttcaactc gcgtcgccgg cacaccggtc aaacccacgg catcggcgat caacgaggcg
     2161 atattgctgt cgccggtaaa ttcctccgga ttcatcaccc cgatgtagag cgcatcgact
     2221 tcttcagttt cgccaagcgc ccgctttgcg gcttcagaaa aaatttcccc aagcgcgcgc
     2281 ggatctttgc caaatcgggt caagccgacg ccagcgacgt aaacggattg catagggcaa
     2341 ttctaacgtc gtgctgcaag gttgcaagat gacagaatta ccgctcaaat ctgacggatc
     2401 agtccaactt ggtttgcgaa ttggcaaaac gattttgccc attcgacgcg cactgacgaa
     2461 tcggcacacg tgagaaatag gttacgcgca gaaaaagttt ttgtgaatga acattcggcg
     2521 ggctaatccg gtgcgcttaa ggctcaacgt ggtttgatta ttccggttgc atcgtctcgg
     2581 tgaaacggtt tccaaccgtc ggcccaatcg aaagcctcca acccaataaa tctctttttg
     2641 taatcatagg cgaagggctc ccgataggca tagccaggat agtaataaca acaaccgcgg
     2701 cgcaaagaaa attcgatcga ccggagcatc atgaaaattc ccaagcttct ttttagctcg
     2761 gccggatcga acatggcata taccgctgaa gtcgcttgtg cgccgacgtc gagaaaggtg
     2821 acgccgctca gccgcgcgcc caaatagacg cagagctcca cattgacgca gggaaccgat
     2881 ccggggatgg tcgacaggaa attgaacagc gaagtcggga cgttttcatc gaagcgaagg
     2941 cgatgttttt cgaacaacgc ttctttttcg ctatccacga acgaagagcg cacgataact
     3001 cgggtgtcgc ggttctttgc cagaactcgt ttttggcttc gcgtagggcg gaaccgggcg
     3061 aggtcaatcc ggagaggaag aacagtgaat tctcggccgc ggtggacagc tttaggatag
     3121 cgcatgaaca caatgccaaa atgacgccag ccttcggccc agacacgatc catttgagcc
     3181 gccgatgcgc tccagcagag aaaactttca tctgccgtcg gtagagtcat agttcaacgg
     3241 cgaaagttca ctactggatg taccagccgg cgggtcgcgc cgatatacgg aacaacgctt
     3301 tgcagcggca aacgaagggc aggatggttt cgggaaatag gcgcgccaaa ttaaaccgag
     3361 aatggacgtc gcgccgaaaa ggaaaatgga ttgtgacact tcaggaatta tcggcagacg
     3421 ctcattacag tttagcggct gtcccgttga gtaaagctcg accagcgggt gtaagcgtta
     3481 cttcgggatc ctgtcttgtc tccgagcgct gtacttgcac ataaccatcg atcatcgcat
     3541 cttcccaaat cgtgtgccgg ggacaagtgc tctgccaggc gttcatgaca tcgccgtaag
     3601 ttcgatggcg gcttgcgacc caggcgagaa actcgagcat tagtaaactg gttggcgccg
     3661 acatgttcac acctcgcgaa tgttcccagt tcagcaaaca ctcggattat aacgcgccgt
     3721 ctcaccgggt tccaatcggc atcctgaagt aaaacggggc gactccaatt gcgctttctg
     3781 ttcactcgct cgaggccaat acgctacggt tttacgggtg gatcgaacgg atcatctcca
     3841 ggtcggtcat caacagccgg atccggatgc caaatcccgc ctcattcgga gcgcggcctc
     3901 ggttccttgg taataaccgg gaacgagatt cagccggtgg taccccatcc gctcataaaa
     3961 ttgctgcgcc ccgctgttac tttctcgaac ttccagggtg atgcgaaaaa ttcccgccac
     4021 ggtggcacac ttctcgagcc attgcagaag ctcctgtcca acaccgaggc gccggtatgg
     4081 cggcgcgacg gcgagcaggt ccaaatgcgc gtcgtcgtcg ccgtaacgca tgatggaaaa
     4141 gccgattatc cgagtctcaa gccgcgcaac gagaacgttg acattggccg cgcaaatact
     4201 ggcttcgacg cgctgagccg tccatttcca ttgcaatccg tattcgatca aagcgcgtga
     4261 catctttgca atcgccggag cgtccgtgcg gcgcgctaaa gataatgaga gatcagaatg
     4321 tgtcatggct tggatgccgg tacacgacag cggactcgaa tccttgctca atccaatccg
     4381 atgtacttaa agcgcatgcc gagtttgccg gcgaaagcat tgatccgcgc tgcagagacc
     4441 aaagccgcaa tccatatgca atttaaaaat gcatcgggcc cattccaata atggcatatg
     4501 caccacatag agaaaaagat gatcgcaaac ttccgcctca gcgtgatgca gcgagatcgt
     4561 ggccagagct tcgcataact cgacaataaa acggcagcag aactgtcgaa tgcgatcgtt
     4621 tgaccagatc gtttatatgc aggaaatttc acgcgccaga aagccccttg ttgcagattt
     4681 acaggctgtg taaaaatgac aggtgctaca tgtatttaac gtcgtcataa cggcgagaag
     4741 ttctgctctc aggtgtaaag gggcacgcat ggcacttttt ttcgctggca tcactcgtgc
     4801 ttccaagtct ctttcacatt atgagcacat cca
//
LOCUS       DASULZ010000325         3259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1461276, whole genome shotgun sequence.
ACCESSION   DASULZ010000325 DASULZ010000000
VERSION     DASULZ010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461276"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..846)
                     /locus_tag="VFY96_06775"
     CDS             complement(115..846)
                     /locus_tag="VFY96_06775"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5606199.1"
                     /translation="MGKTILVTGGAGGICSDICRGLAADGLNVVVADYAHDEAEKVAT
                     EIRENKGDAMAVKVDVGDPASVTEMMSRAIAKYGQIDYAFCGAGVMDRVPVIEMPEQV
                     WDRLMRINLKGVFLCAQAAAKHMIPRKEGRILSIASGRGVAGAPRSAHYAASKAGVIA
                     FTKSLAVELAPDNITVNCVCPGATDTPMSRIGFTPEQFKKREEIAPLMDGLTHKFEIV
                     GLVRYLLSDATKFVTGQTFFLRTPK"
     gene            complement(951..1859)
                     /locus_tag="VFY96_06780"
     CDS             complement(951..1859)
                     /locus_tag="VFY96_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263046610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEX5606200.1"
                     /translation="MRICSFLPGATEIAFALGLGDQIVGVTHECDYPAEAKQKPVVVR
                     SAIDSHRLSGHEIDRKVGELLEAGKGLYLIDDEALRASAPELILTQGLCDVCALDYNE
                     VVKAAAGLTYVPIILSLNPHSLADVLDDVVRIGAATNSAAAADAFVSELRQRVEQIGL
                     GEPNYRPRVVCLEWFEPLYIAGHWVPEMVALAGGHDVLGRLGEPSFRIEWSSVLDADP
                     EIILLMPCGFDVRRAVKEASALRRLPRWEELPAVKGGRVYALNGSAYFSRPGPRLVNG
                     LEILARIIHPETVAWSIPSADAAIIN"
     gene            complement(1940..2716)
                     /locus_tag="VFY96_06785"
     CDS             complement(1940..2716)
                     /locus_tag="VFY96_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019565597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX5606201.1"
                     /translation="MNRKISRRNLLIGALQAASLGLLSGCEKLFDSLSQNKNVQSLLE
                     IAEDGSRRAQRLLTRRNKLAKEFEPQDISPKFRPNGNPAPITMDYTTDAQNHWTKWRL
                     EVSGLVKQPASFSLADLQAMPSRTQITRHDCVEGWSVIGKWKGVRLEEIINRVQPGSD
                     AKYVVFRCMDTDSDGVNYFESIDMIDAVHPQTILAYELNDQPLPIDNGAPLRLRVENQ
                     LGYKHAKYIRALEFVSSLDKVGQGKGGYWEDQGYEWYAGI"
     gene            complement(2706..>3259)
                     /locus_tag="VFY96_06790"
     CDS             complement(2706..>3259)
                     /locus_tag="VFY96_06790"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX5606202.1"
                     /translation="RRWHFFFGWLFAISGAVYLIYNLAVGHIRKFLFTLRDAKKLPAM
                     TAYYLHIRRISPQEGEYNPLQKMAYSGVFVILAPLILLSGMAMSPQLNVAFHWLPATF
                     GGRQSARAVHFILAFGFLFFVLGHVFMVLTQGFLNNMRSMTSGWYREKVPVMIESTAE
                     AAPHAAPPVSSKQEAEKSNEDES"
BASE COUNT          698 a          926 c          913 g          722 t
ORIGIN      
        1 gcatcagcgg agtttcacac agtctggcgc tcgagatgac aagtacgggg ccatcggggc
       61 gcgttacgac ccagctttta cgcactgctg atatgaaaat aactggatgt cttcttattt
      121 cggagtgcgc aggaagaacg tctgacccgt gacgaatttc gtggcgtcgg aaagcagata
      181 gcgcaccaac ccgacgatct cgaatttatg tgtgaggccg tccatcaagg gcgctatctc
      241 ttcgcgcttc ttgaactgtt ccggagtaaa accgatcctc gacatcggcg tatcggtcgc
      301 tccggggcaa acgcaattga ccgtgatatt gtccggggcc aattcgacgg ccaatgattt
      361 tgtaaaggcg attacgccgg ccttggatgc ggcgtaatga gccgatcggg gagcgccggc
      421 aactccacgg ccggaagcga tgcttaaaat tcgcccttcc ttgcgcggaa tcatgtgttt
      481 tgctgccgcc tgcgcgcaaa gaaaaacgcc cttcagattg attcgcatca agcgatccca
      541 gacttgttcc ggcatctcaa tcaccggcac gcggtccata actccggcgc cgcagaacgc
      601 gtagtcgatt tgaccgtatt ttgcgattgc tcggctcatc atctctgtga cgctcgcggg
      661 atcgccgaca tccactttca ctgccattgc atcgcctttg ttttcgcgga tttccgtggc
      721 aactttttcg gcctcatcat gcgcataatc cgccacgacg acgttcagtc cgtccgcggc
      781 gagccctcgg cagatatcgg agcagatgcc cccagctcca cccgtaacga gaattgtttt
      841 gcccatgtgg aacgctcctc gtgcaaagct ttgctgacgc taccattcgc gcttggcggc
      901 ggtcaaacct acgcgcgggc ggcagctttt tgcggcgatt gtgtcagtac tcaatttatt
      961 atggctgcgt ccgcggaagg gatcgaccag gcaaccgtct cggggtggat gatgcgggcg
     1021 agaatttcca acccgttcac caagcgcggt ccggggcggc tgaaataggc gctgccgttc
     1081 agagcataga cgcgtccgcc ctttacggcc ggtaattctt cccagcgtgg aagtcttcgc
     1141 agcgcgcttg cttcttttac cgctcggcgc acgtcgaacc cgcagggcat gagcaaaata
     1201 atctcgggat cggcatcgag cacgctcgac cattcaattc taaacgatgg ctcgcctaaa
     1261 cgcccaagga catcatggcc gccggcaaga gccaccatct cagggaccca atgacccgca
     1321 atgtacagcg gctcaaacca ttcgaggcaa actacgcgag gccgatagtt gggttcgccg
     1381 agcccgatct gctctacgcg ctggcgaagt tcgctgacga acgcgtcggc cgctgctgca
     1441 gaattcgtcg ccgcgccgat gcgcacgacg tcatcgagca catcggcgag cgaatgtgga
     1501 ttgagagaga gtatgatggg tacataggtc agtcctgcgg cggctttgac aacttcgttg
     1561 taatcgaggg cacagacgtc gcatagtcct tgggtgagaa tgagttccgg cgcgcttgcg
     1621 cgcaatgcct cgtcgtcgat caggtagagt cctttgccgg cttccaggag ctcaccgact
     1681 ttgcgatcga tctcgtggcc ggaaagacga tggctatcga tggcgctccg caccacaacg
     1741 ggtttttgct tcgcttcggc cggataatca cactcgtgag tgacgccgac aatctggtcg
     1801 ccaaggccga gcgcaaacgc gatttccgtt gcgccgggga ggaaagagca gatgcgcatc
     1861 agctatgaat agctcaatcg atgacaagat gccactgctc cgccatgccg caagagctat
     1921 ttaaaattgg cggagggaat tatattcctg cgtaccactc gtaaccctga tcttcccagt
     1981 agccaccttt tccctgcccg accttatcca ggctggagac gaattcgagg gcgcgaatgt
     2041 atttggcgtg cttatacccg agttggtttt cgacgcgcaa acgcagcggc gcgccgttgt
     2101 cgatcggcag cggctgatcg ttcaattcgt aggcgagaat cgtctgtgga tgcacggcat
     2161 cgatcatgtc gatgctctcg aaatagttga cgccgtcgct atccgtgtcc atgcagcgaa
     2221 agacgacgta tttggcgtca ctaccgggct gaactcgatt gataatctct tccaggcgca
     2281 cgcctttcca cttgccgatg acgctccagc cttcgacgca atcgtggcgc gtgatctggg
     2341 tgcgcgacgg catcgcttgg agatccgcca gtgaaaaact cgccggctgc ttcaccaaac
     2401 cggaaacctc gaggcgccac tttgtccaat gattttgcgc gtcggttgtg tagtccatcg
     2461 tgatcggcgc ggggttgccg ttggggcgga acttcggcga gatatcttgg ggctcaaatt
     2521 ccttagccag cttattgcgt cgagtaagca ggcgctgcgc gcgacgactg ccatcctcgg
     2581 ctatctcgag cagcgattga acgttcttgt tctggctgag gctgtcaaag agtttttcgc
     2641 agccgctcaa aagacccaag ctcgcggctt gaagcgcgcc gataagcaaa ttgcgtcgcg
     2701 aaattttacg attcatcttc attgcttttc tccgcctctt gcttcgatga aacgggtggc
     2761 gctgcgtgcg gcgcggcttc ggctgtcgat tcgatcataa cgggaacctt ttctctgtac
     2821 cagccgctgg tcatcgatcg catattattg agaaagcctt gggtcagaac catgaagacg
     2881 tgtccgagga caaaaaacag aaacccgaac gccaagatga agtgaaccgc gcgcgccgat
     2941 tgcctgccgc cgaacgtcgc gggcagccag tggaaggcaa cattcaactg cggcgacatc
     3001 gccatgccgc tgagcaagat caacggagct aagatgacaa agactccgct ataggccatt
     3061 ttttgcagcg gattatactc gccttcctgc ggcgagattc ttcttatgtg caggtagtac
     3121 gcagtcattg cgggcaactt cttggcgtcg cgcagagtaa acaagaactt tcgaatatga
     3181 ccgacggcga ggttatagat gaggtagaca gcaccgctaa tcgcgaacaa ccaaccgaaa
     3241 aagaaatgcc agcgccgtc
//
LOCUS       DASULZ010000326         5475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_954806, whole genome shotgun sequence.
ACCESSION   DASULZ010000326 DASULZ010000000
VERSION     DASULZ010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5475
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954806"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            446..1720
                     /locus_tag="VFY96_06795"
     CDS             446..1720
                     /locus_tag="VFY96_06795"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX5606203.1"
                     /translation="MITAAPPSSTQTSGEENDFVKHARAIHERVLTLDSHIDFAPADL
                     ISERNYTQRLDTQFNLPNMIDGGLDGLFFIIFVAQTRESQNAEALKSPGYERAYHRAL
                     EKFDAVHHFTHAIAPDKIELAFTAVDARRIHAAGKKIAFMGVENGYALGEDLRLVKEF
                     YDRGARYLSLTHNGHNQLADSHTGEREGWKWHGVSPLGEEVIAAMNRLGIMVDVSHAS
                     KNAMMQIAALSHAPIIASHSAVRALCDVSRNLDDEELLALRKTGGVVQVVAYNGFIKT
                     TKPDSPERLSALAALRKEYDIPETNGPGQRARLPSALQEWPDERRHQFESRLAAIDKQ
                     YPDDRPATVKDFVDHIDYAVNLIGIDHVGISSDFDGGGGVIGWNDASETFAVTLELVR
                     RGYNEEQIAKLWSGNLLRVMDEVQRIADELKE"
     gene            1892..2203
                     /locus_tag="VFY96_06800"
     CDS             1892..2203
                     /locus_tag="VFY96_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HEX5606204.1"
                     /translation="MRYLLKVNIPVESGNAAAKAGKLGATIQSILADLKPEAVYFADD
                     KGQRTAYIFLEMQNAAQIPAIAEPWFLAFNASLELHPVMTPEDLAKAGSAIESAVKKY
                     G"
     gene            2231..2632
                     /locus_tag="VFY96_06805"
     CDS             2231..2632
                     /locus_tag="VFY96_06805"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5606205.1"
                     /translation="MPIRSFDHVAIPIQNTAAMLSFYRALGFGIQEKEHFYSVHFGDQ
                     KINFHAPERWQSGRFTLRGPTAVPGCGDFCFVWEGSAESLHATLDQAGAKIIEGPIER
                     MGGRDHGRTQGTSIYVRDPDSNLLEFMIYPD"
     gene            2865..3254
                     /locus_tag="VFY96_06810"
     CDS             2865..3254
                     /locus_tag="VFY96_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEX5606206.1"
                     /translation="MALRIVRLGTPRHAKEGLRIGTVRHPPRGVLKKDYARRNYFDVW
                     LPELAPSAPLVSWFFSEPLTAKHWSDYARRYRREMQKPAARRLIELLAALSLKTNFSV
                     GCYCEDESRCHRSLLKMLLLEHDAKIR"
     gene            complement(3437..4450)
                     /locus_tag="VFY96_06815"
     CDS             complement(3437..4450)
                     /locus_tag="VFY96_06815"
                     /inference="COORDINATES: protein motif:HMM:NF040043.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HEX5606207.1"
                     /translation="MCDFAADAKAAEELMVGRTLTVARVRELNAKDYFYQMLKDNPEM
                     LKIYPGIETELLQGAIDCHIHAYPDFVHRSQDMIQIAIEASKCGMRAIAFKDHWNISA
                     TSAYLTQRHIDDMIARGELTHRVEVYGGVGMCFGMRPEYVRVGLQYPNFKMIWFPTFT
                     SYGFWRGAGHPDHEGVRLCSEDGQVLPEVKQIMEMAAEKKVGIGFGHTDFQELLPLGR
                     LAKDIGVRATLDHPLLELNKLLLDEMKQLRDLGVYVGTYCQPMIPSLYQPVADPMETI
                     RTIKEIGPDRCIIGSDFGQVLHMDAVDGMRVFIRALLGFGIKPDEVKIMLKDNPAKLM
                     WLD"
     gene            complement(4537..>5475)
                     /locus_tag="VFY96_06820"
     CDS             complement(4537..>5475)
                     /locus_tag="VFY96_06820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5606208.1"
                     /translation="IVLAEQVGFGAFWKGESNSTDPMIMLSAVASRTKTIKLGTAIYH
                     IYGRSPVTLGIQSATLQDLSRGRLLLGLGVANKSIAGWHGGVFDRPLKRAREYIEIVR
                     KVAAGERVEYEGEIYQTGKRFQLSWKPNHPNLPVYLAGLGPQMTKLVGKISDGVFINM
                     ATPAKVKEIAARVREGALEAGRDPNKIEIIAKSRVSLNPDKSLARAKLRQVLTFYNIA
                     DHYSDMLRGMGFEKEVNAIHEAFQKGGFKAAMGALTDDYMDKLPVVPASDIREIKEKM
                     KQFEEAGVTRMVIPYVPVTEPVVEDARKFFEAWGNS"
BASE COUNT         1224 a         1596 c         1439 g         1216 t
ORIGIN      
        1 aatcccagct aacaatgtgt aagaaattag atccgacatc ttgttgccct tcgttgaatt
       61 cgatttcgtt ctttgcagag tagcaggtga ggagaggcaa aacgcaagcc attgtcctgt
      121 tttctaagta gttcgaacac aagcaaccgc aagagagtaa attcatcgct tggtcagttt
      181 tgtgcagatc taaacattcc ggcatacgtc aatagcagct ctcggcccac cagtagtcag
      241 tcgaagcacg actcggaaac actatcatgc acttcgttag cgttcgaagc agcctcgtgc
      301 tgccattgtt gcttcctgat cggcgcgatc gcgttacgcg cccgcgatat ggatcgcccg
      361 tccacaatct gctagtctaa gcgcacgtca ttatccatcg cgctaagtac tctagtcagc
      421 aaagtttatc tctgcaccac tcgaaatgat aactgccgcc ccgccgagct caacgcaaac
      481 ttccggcgaa gaaaatgatt ttgtaaagca cgcgcgggcg attcacgagc gagtgctcac
      541 tctcgacagt cacatcgact ttgctccggc tgacctgatt tccgagcgca actacacaca
      601 gcgtctcgac actcagttta acttaccgaa catgattgac ggcggtctcg atggcttgtt
      661 ttttatcatc tttgtcgccc agactcgcga gtcgcaaaat gccgaagccc tcaaatcccc
      721 gggttacgaa cgggcttatc atcgcgccct cgaaaaattc gacgcggtcc atcacttcac
      781 gcacgcaatc gcgccggaca agatcgagct ggcattcacg gctgtcgacg cacggcgaat
      841 ccacgcagcg ggaaaaaaaa tcgccttcat gggcgtcgaa aacggctatg cgctcggcga
      901 agacttgcgc ttggtgaagg agttttacga tcgcggtgca cgctatttgt cgctcaccca
      961 taacggacac aatcagcttg ccgactcgca cacgggcgag cgcgagggct ggaaatggca
     1021 cggcgtttcg ccgctcggcg aagaagtcat cgcggcaatg aatcggctcg gcatcatggt
     1081 ggacgtctcg cacgcctcga aaaacgcaat gatgcaaatc gcggccttgt cgcacgcgcc
     1141 gatcatcgca tcgcattccg ccgtccgcgc gctctgcgat gtgagtcgca atctggatga
     1201 cgaggaattg ctggcgctcc gaaaaaccgg cggcgtcgtt caggtggtcg cctacaatgg
     1261 atttatcaaa acgaccaagc ctgattcgcc ggagcgactc tctgcgctcg cggcgctccg
     1321 aaaagaatac gatattcccg agaccaacgg cccgggccaa cgcgcgcgtt taccatcggc
     1381 tctacaagaa tggcccgatg agcgccgcca tcaattcgaa agcagactcg ccgcgattga
     1441 caagcaatat ccggacgaca ggccggcaac agttaaagat tttgttgacc atatcgatta
     1501 cgccgtcaat ttgatcggca tcgatcacgt cggcatttcc tccgatttcg atggcggagg
     1561 cggcgtgatc ggatggaacg atgccagtga aacattcgct gtcacgctcg agctggttcg
     1621 ccgcggctac aacgaagagc aaatcgcaaa gctttggagc gggaacctgc tccgggttat
     1681 ggacgaggtc cagcggattg cagacgaact aaaagagtaa gtcgtttgac gcgcgcccta
     1741 cgcgcgcaga cagataacat cgggcgcgaa ttcatttgac agttgccaca cgtcgcgtga
     1801 aattagcttt cgtaagccgc gagcattcaa gcccgtagct ctgcagtatc tagaatttgt
     1861 gaattagaaa cgaagtgtgt ggaggtagac catgcgatat ttattgaagg tgaacattcc
     1921 cgtcgagtcc ggcaatgctg ccgcgaaagc cggcaaactc ggcgcgacga tccaatctat
     1981 tctggcagac ctgaagcccg aggcggtcta ttttgctgac gacaagggac agcgaaccgc
     2041 atatattttt ctcgagatgc aaaacgcggc acaaatccca gcgatcgccg agccgtggtt
     2101 cctcgcgttt aacgcaagcc tcgaactgca tccagtcatg acgcccgaag atctagccaa
     2161 ggccggcagc gccatcgaga gcgcagtgaa aaaatatgga tgatttcctc ttgaagctga
     2221 aaggctcgca atgccgattc gaagttttga tcatgtcgca atacctatcc agaacaccgc
     2281 tgcgatgctc agcttctacc gtgccctggg attcggtatt caggaaaaag aacattttta
     2341 ttcggtccac ttcggcgatc agaagatcaa ttttcatgca cccgagcgct ggcagagcgg
     2401 gcgcttcaca ctgcgcggac caaccgcggt cccgggctgt ggcgatttct gtttcgtgtg
     2461 ggagggctcg gcggagtcgc tgcatgccac cctcgatcaa gccggcgcta aaataatcga
     2521 ggggccgatc gaacggatgg gcgggcgcga ccatgggcgc acacaaggca caagcattta
     2581 tgtgcgcgat cccgattcga atctcctgga gttcatgatc taccccgatt gacggaactt
     2641 cgctacgatc gccgtattca aactcggcgg cgaaattact catacgccgt cgtcgccccc
     2701 gagcgtctta atcgggggcc caggtccgat ccaacctgga ttcccgctta aaccatgcgg
     2761 gaatgacaat ctgcttttcg ggatactgta aagtgttgca catgggttcg cgtcttcctc
     2821 gttcgggaat tgcggatgca cagcagaagg attaaccagt actcatggcg cttcgaattg
     2881 tccggctcgg aactccgcgc catgcaaagg aaggattgcg catcggcacc gtccggcacc
     2941 cgccgcgcgg cgtactcaag aaagattacg ctcggcgcaa ctatttcgac gtctggctgc
     3001 ctgaattggc gccgagtgcg ccgctcgttt cttggttctt ttctgaaccg ctgacggcca
     3061 agcactggag cgattatgct cggcgctacc gaagggaaat gcaaaaaccg gcggcgcgca
     3121 ggctcattga attgctcgcc gcgctttcat tgaaaacaaa tttctcggtg ggctgttact
     3181 gtgaagacga gagccgctgc cacagatcgt tattgaagat gctcctgctc gagcacgatg
     3241 cgaagatcag gtgaaggcga ccgccggttt ccccttcgat ctaaaaggtc gcagcatgct
     3301 gcgaccctac ctcttgccgt tcatcctccg tagcgcgtcc ttcgattcgc gctcccctcg
     3361 ctatgggctt cgcgctacgc ggggcggccg gtcgtcctga gtaggcggca cagccgccgt
     3421 atcggcggat cgccttctaa tccagccaca ttagcttggc cggattgtct ttgagcatga
     3481 tttttacttc gtccggctta atgccgaacc caagtaacgc gcggatgaag acgcgcatac
     3541 catcgaccgc gtccatgtgc agcacctgac cgaagtcgct gccgatgatg cagcggtcgg
     3601 gaccgatctc cttgatcgtg cgaatggttt ccatcggatc ggcgaccggc tgataaaggc
     3661 tcgggatcat cggctgacaa taggtgccga cataaacgcc cagatccctc agttgcttca
     3721 tttcgtcgag cagcagcttg ttcaactcga gcagcgggtg atccaacgtc gcgcgcaccc
     3781 cgatgtcttt ggccaggcga ccgagcggca acaattcttg aaaatccgtg tggccaaagc
     3841 cgatgcccac ttttttctcc gctgccattt ccatgatctg tttcacttcg ggcagcactt
     3901 gaccgtcctc actgcataag cgcacgcctt cgtgatcggg atgtccggcg ccgcgccaga
     3961 agccatagga ggtaaacgtc ggaaaccaga tcatcttgaa attcggatac tgcaatccga
     4021 cacggacgta ttcggggcgc atcccgaaac acatgccgac gccgccataa acttccaccc
     4081 gatgcgtcaa ctcaccgcgc gcaatcatat cgtcgatgtg gcgctgcgta agatacgcgg
     4141 aggtcgcgct gatattccaa tgatccttga atgcgatcgc gcgcatgccg cacttcgatg
     4201 cttcgatcgc gatctggatc atatcctgcg agcggtgcac gaaatcagga taggcatgaa
     4261 tgtgacaatc gatggcgccc tggagcaact cggtttcgat ccccggataa attttgagca
     4321 tctccgggtt gtccttcaac atctgataaa aatagtcttt ggcgttgagc tcgcgcactc
     4381 gggcaaccgt caacgttctt cccaccatca attcttcggc tgccttcgca tctgcggcaa
     4441 aatcacacat gaaaatctcc ctttactgga ttttagtctc gggtcccgag tctcgcgtct
     4501 agagttcgga cctaaacgcc aaacccgaga acgcttttac gaattacccc aagcttcaaa
     4561 aaatttgcgg gcatcttcga cgaccggttc ggtgacggga acgtacggga tcaccattcg
     4621 cgtgacgcca gcctcctcga actgtttcat cttttccttg atctcacgaa tgtcgctggc
     4681 cgggaccacg ggcagcttgt ccatatagtc atcagtgagc gcgcccatcg ccgctttgaa
     4741 gccgcctttt tgaaacgctt catgaatggc attgacttct ttctcgaaac ccatgccccg
     4801 cagcatatcg ctatagtgat ccgcgatgtt gtagaaggtc agcacctgcc gcagcttggc
     4861 acgcgccaag cttttgtccg gattcagcga cacgcgcgat ttagcgatga tctcgatctt
     4921 gttcggatcg cgtcctgctt cgagcgcgcc ttcgcgcacg cgtgcggcga tttctttgac
     4981 cttcgccggc gtcgccatgt tgatgaacac gccgtcggag atcttgccca ccagcttggt
     5041 catttgcggc cccagaccgg cgagatatac cggcaggttc ggatgattcg gtttccaaga
     5101 aagctgaaac cgcttgccgg tttgatagat ttcaccttcg tattcgacgc gttcgccggc
     5161 ggccaccttg cgcacaatct caatatactc ccgggctctc ttcagcggcc gatcgaacac
     5221 gccgccatgc caccccgcaa tgctcttgtt tgccacgccg agaccgagca gcaacctccc
     5281 tctagaaaga tcttgcaagg tcgcagactg aatgccgaga gtcaccggac tccgaccgta
     5341 tatatggtaa atcgcggtgc cgagcttgat cgttttggtc cggctcgcaa ccgctgaaag
     5401 catgatcatc ggatcggtgc tgttcgattc gcccttccaa aacgcgccga agccgacctg
     5461 ttctgcgagc acgat
//
LOCUS       DASULZ010000327         6806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1258886, whole genome shotgun sequence.
ACCESSION   DASULZ010000327 DASULZ010000000
VERSION     DASULZ010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6806
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258886"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VFY96_06825"
     CDS             <1..434
                     /locus_tag="VFY96_06825"
                     /inference="COORDINATES: protein motif:HMM:NF019272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEX5606209.1"
                     /translation="RWTKRAPMLTPRDHLAAGVVNGKLYAIGGRINGRHDRSITANEE
                     YDPAANRWITRSPLPTVRSGIAAAVLEQKVFVFGGESTRGTHKEVESYDPSKNLWQRW
                     APMPTARHGLGVALLGRSIYVIAGGPQPGATYSSVNEVFTP"
     gene            complement(560..1546)
                     /locus_tag="VFY96_06830"
     CDS             complement(560..1546)
                     /locus_tag="VFY96_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5606210.1"
                     /translation="MDPKHCEPSFASGPYRAPWWLPGGNLQTIYARWLARSYSVSYRR
                     ERWDTPDHDFIDLDWVDHPNGDKIIVLFHGLEGYSQSHYARSLMAKATEFGWRGVVPH
                     FRGCSGEANRLLRSYHSGDSDEIDWILRRIKTETPHSEIYAVGISIGGNMLLKWLGEQ
                     GECASRIIERAVAVSTPLDLVAAAGELDFGIKRVIYASHFLRTLRPRALTKVVAHDLA
                     IDPRAIRASITFRQFDDLFTAPVHGFQNAADYWTRCSSKPWLSKIAISTLVLNAQNDP
                     FLPASALPAADEVSSAVTLEFPPSGGHVGFVTGKFPGRLDWLPQRILRFFAD"
     gene            complement(1568..4534)
                     /locus_tag="VFY96_06835"
     CDS             complement(1568..4534)
                     /locus_tag="VFY96_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX5606211.1"
                     /translation="MANKNISVESIEPEVQRLARTLFQKAAEHKPTLFETRDLLGRMI
                     EWSLEDESLRVALFRFVDVLPSLDSSAEIGRHLEEYFARVDHALGGLALLAQALHAGT
                     LMAPVVRRNVVKLARRFIAEESGDHLIKAITALRKEPAAFTLDAVGEATVSDDEAAAM
                     QQRYLDLLRRLSAVSERWPSCAQIDESDNGTIPRVNLSVKLSSLCARFDPLDPTTEAT
                     VRRRLRELLRQAAKVKAAITVDMEQYAFKELTLEIFQRVLAESEFRGGPHAAVALQAY
                     LRDSERDVRELITWAREHGRRVGVRLVKGAYWDSEIAWAQQKGWPIPVYVEKAETDAS
                     YERLSRILLESRDVIDAAFGSHNLRSLAHAITTAKRLGISASGFEIQMLYGMAEPVRR
                     AIIANGQRVRVYLPVGELLPGISYLIRRLMENTSNTSFLRQTYADRKDINRLIQPPII
                     TETPSRRSRTPGRSEGGDRREFRNEPLIDFSRRENRELFGQSLQKVREEFSGQAGQRI
                     DGQTLASLNPANSAEIIGRVRMATSSDVESDVETATRFFPRWRSETAPERAKFLFAAA
                     EIMRQRRWELAAWEVFEVGKSWREADADVAEAIDYLEYYGRQMLHLALPRETQRLQSE
                     SNVYLYEPRGLAAIIAPWNFPLAILTGMTAAALVTGNCALMKPAEQSPVMAVRLLEIL
                     REAGVPPQACRLLQGGGEIGASLVRSPRIHLIAFTGSREVGLEILQQAYTHRAGEDHV
                     KRVVCEMGGKNAVIVDNDADMDEAIVHVIDSAFGYQGQKCSAASRLILVEDIYERFLT
                     RLIEATKSLQIGPPEDARNTVGPLIDAEAKKRVLEYVRVGKTEATCVLEMLAPDEGFF
                     VGPVIFRDVEPQNRIAQEEIFGPVLGVMKAANFEDALAIANQSAFALTGGVFSRSPAH
                     IEKARQEFRVGNLYINRSITGAVVERQPFGGLKLSGIGSKAGGPDYLLQFLEPRTISE
                     NTLRHGFVPPDNVRQK"
     gene            4897..6597
                     /locus_tag="VFY96_06840"
     CDS             4897..6597
                     /locus_tag="VFY96_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5606212.1"
                     /translation="MTRPDEQHLETLTGAEAAVEMLKAHGVEILFGLCGDTSLPFYDA
                     LARLPHGMRHVLTRDERSASYMADGYARVSGKVGVCEGPSGGGATYILPGLAEANESS
                     VPLLCINSDISVAARGRYTLTELDQSALMRPVTKWNSVLNRAEDLPRIFRAAFRQITT
                     GRPGAAHIALPFDVQNGSVNRAEVWGDVRFGRYPAERIAPDSESVEYATHLLRAAKKP
                     LLICGGGVILSQAQDELVALAEQLSIPVTTTISGKGSIDEEHPLAAGVVGSNGGTPET
                     RAIVDNADLVFFIGCRAGSVTTERWRHPRPGGCKVIHLDVDPAVPGANYRTDAALICD
                     AKLGLKMLREALANQRRVVNGSDLESAKERKFAKFQVLAESLDKPIKPERLVAELTRV
                     LDRDAILVADAGTPCPYFSAYYPVRRSGRCFSNRAHGALGYSLPAAIGAHFARPSVKC
                     VSVMGDGSFGFTCGELETAVRYCLPITFIVISNASYGWIKAGQKSGYSQRYFSVDFAA
                     TDHAAVAAAFGVKSWRVTEPTELGKHLKDALAYGGPTLVDVVCQSLEEAKAPVSEWIA
                     "
BASE COUNT         1426 a         2056 c         1871 g         1453 t
ORIGIN      
        1 accggtggac gaagcgcgcg cctatgctca cgccgcgtga tcaccttgcc gccggagttg
       61 tcaacggcaa gctttacgcc atcggggggc gcatcaatgg ccggcacgat cgcagcatca
      121 ccgcaaatga agagtatgat ccggcggcga atcgatggat cactcggtca ccgttgccga
      181 cggtccgtag cggcatcgct gcggctgttc tcgagcaaaa agttttcgtc ttcggcggcg
      241 aatcaacaag agggacgcac aaagaagtgg aatcctacga tccctcgaaa aatctctggc
      301 aacgctgggc gccgatgccg acggcgcgcc atggtctagg cgtcgcgctc ctcggcaggt
      361 ctatttacgt tatcgccggg ggcccgcagc ccggtgcgac atattcatcg gtcaacgaag
      421 ttttcacacc ctagaatcac ccgatccgct gagtctggtt ttcataatga tcttcaaggc
      481 cgtggtgtct tgacatcaaa atacgcaaca aagtggatcg tcattccggg caagctagca
      541 tagctagcgc gacccgtaat cagtccgcga aaaaccgcag gattctttgc ggcagccagt
      601 cgaggcggcc cggaaattta ccggtgacga atccgacatg gccgccggaa ggcggaaact
      661 cgagcgtcac tgcactggaa acttcgtctg ccgcaggcag tgccgaggcc ggcaggaacg
      721 gatcattctg tgcgttcaga accaaggtcg agatcgcgat tttgctgagc caaggcttgc
      781 tgctgcaccg cgtccagtaa tcagccgcgt tttgaaaacc gtgcaccggc gcagtaaaca
      841 ggtcatcgaa ttgtcgaaac gtgatgctgg cgcgaatcgc tcgcggatct atcgctaaat
      901 catgagcaac gactttcgtt agtgcgcgcg gccgcagagt ccgtaaaaaa tggctcgcat
      961 agatgactct tttgatgccg aaatcgagct cgcccgccgc agccactaga tcgagcggtg
     1021 tcgatacggc gactgccctt tcaatgatcc gagaagcgca ttcgccttgt tcaccgagcc
     1081 atttgaggag catgttaccg ccgatcgaga tgccaaccgc ataaatttca ctgtgtggag
     1141 tctccgtttt gatccggcgc aagatccaat cgatctcatc ggaatcgccc gaatggtacg
     1201 agcgcagcaa ccggttggct tcaccgctgc aaccgcgaaa gtgcggcacg acgccgcgcc
     1261 agccgaactc agtcgccttg gccatgagac tccgtgcgta atgactttgc gaatagcctt
     1321 ccaacccatg gaacaggaca atgattttgt cgccgttcgg atgatcgacc caatcgagat
     1381 cgataaagtc gtgatccggc gtatcccagc gctcgcgccg atacgaaacc gaataacttc
     1441 gcgccagcca acgtgcataa atcgtttgca ggttccctcc aggcagccac catggcgcac
     1501 gataaggtcc tgacgcaaac gaaggttcac agtgttttgg atccatcggt ttgaacggtt
     1561 tgaacttcta tttttgcctc acgttgtccg gtggtacgaa tccgtgacgc agggtatttt
     1621 ccgaaatcgt tctcggctcg agaaattgca gcagataatc cggtccgccc gccttcgaac
     1681 cgatcccgga aagcttgagg ccgccgaacg gttggcgctc caccaccgcg ccggtaatac
     1741 tccgattgat atagagattg ccgacacgga attcctggcg cgccttttca atatgcgccg
     1801 gggagcgcga gaacacgccg ccggtcagcg cgaacgcaga ttggttcgcg atcgccaggg
     1861 cgtcctcaaa gttagcggct ttcatgaccc cgagcacggg tccaaagatt tcttcctgcg
     1921 cgatgcggtt ttgcggctcg acatcgcgga aaatcactgg accgacgaag aatccttcgt
     1981 cgggcgcgag catttccaag acgcacgtgg cttccgtttt tcccactcga acatactcca
     2041 agacgcgttt cttagcctcc gcatcgatca aaggtccgac cgtgttgcgc gcatcttccg
     2101 gaggaccgat ttgcaggctc ttcgtcgctt cgatgaggcg cgttaaaaat cgctcataga
     2161 tatcttcgac taagatcaag cgcgaggcgg cggagcattt ttgaccttgg tagccaaagg
     2221 ccgagtcgat aacatgaacg atcgcttcgt ccatatcggc atcgttgtcg acgatgacag
     2281 cgttcttacc gcccatctcg cacaccaccc gcttcacgtg atcttcccct gcacgatgcg
     2341 tataggcttg ctgcaaaatc tccagaccga cttcgcgcga gccggtaaaa gcgatcagat
     2401 gaatccgcgg cgagcgcacc agagacgcgc caatctcacc accgccttga agcaaccggc
     2461 aagcctgcgg tggtactccc gcttcacgca gaatttccag caggcgcacc gccataaccg
     2521 gcgactgctc cgccggcttc attagagcgc agttaccggt caccagtgcg gccgcggtca
     2581 tcccggtaag gatcgccagg ggaaaattcc acggcgcgat aatcgccgcg agcccgcgcg
     2641 gctcgtataa atagacgttg ctttcgctct gcagccgttg cgtttcgcgg ggcagcgcca
     2701 gatgcaacat ttgccgcccg taatattcga gataatcaat cgcctcggca acatccgcat
     2761 ccgcttcgcg ccagcttttt cccacttcga aaacttccca tgcggcaagc tcccagcggc
     2821 gctgccgcat aatctcggca gcggcaaata gaaactttgc gcgctccggc gcagtctcgg
     2881 aacgccagcg cggaaaaaag cgcgtcgccg tttcgacgtc actttctaca tcactgctcg
     2941 tggccatgcg cactcgtcct ataatttcgg cggaattggc tgggttgagg gacgcgagtg
     3001 tttgaccatc gatccgttgt cccgcttgac cgctgaattc ctcgcgaact ttctgaagcg
     3061 actggccgaa gagctcgcgg ttttcacgcc gggaaaaatc gatgagcggc tcattgcgaa
     3121 attcccgccg gtcaccgcct tcgctccgcc cgggtgttcg gctccgtctg cttggcgttt
     3181 cggttattat cggcggctga atcagacgat taatatcctt ccgatccgca taggtctggc
     3241 gcaggaaaga ggtattcgaa gtattctcca ttagccgccg aatcaggtat gagatccccg
     3301 gtaacaactc gcccaccgga agataaaccc gcacgcgctg gccattggcg ataatggccc
     3361 ggcgcaccgg ctccgccatg ccatagagca tttgaatttc aaatccgctg gcggaaatgc
     3421 ccagccgttt cgccgtcgtg atcgcatgcg ccaagctgcg caagttgtga ctgccgaagg
     3481 cggcatcgat gacatctcgg gattcgagta gaatccggct cagcctctca tagctggcgt
     3541 cggtttccgc cttttccaca taaaccggaa tcggccagcc tttttgctgt gcccaagcga
     3601 tttcgctgtc ccaataggcg cccttgacta atcgcactcc gactctgcgc ccgtgctcgc
     3661 gcgcccaggt gatcagctcg cgcacgtctc tttcgctatc acgcagataa gcttgcagcg
     3721 cgacggccgc gtgcgggcca ccgcgaaatt cgctttccgc caagaccctc tgaaaaatct
     3781 ccagggtcaa ttccttaaaa gcatattgtt ccatgtcgac ggtgattgcc gctttcacct
     3841 tcgcagcttg gcgcagcaat tcacgcaatc ggcggcgcac ggttgcttcc gtggttgggt
     3901 cgagcggatc gaaacgcgcg cagagcgaag aaagcttcac cgagagattc acccgcggga
     3961 ttgtgccgtt atcggattcg tcaatctgag cgcaactggg ccagcgctcg ctcactgcgg
     4021 ataagcgccg gagcaggtcg agatagcgct gctgcatcgc cgccgcttcg tcatcgctta
     4081 ccgtggcctc cccgaccgca tccagcgtaa aagctgcagg ctccttcctc agcgcggtga
     4141 tcgccttgat caggtgatcg ccactttctt cggcaataaa tcgccgcgcg agcttcacca
     4201 cgttgcgccg caccaccggc gccatcaagg ttcccgcgtg cagcgcctgt gctaacagag
     4261 ccaggccgcc gagcgcatga tccacacgcg caaaatactc ctccagatgg cgaccgatct
     4321 ccgccgacga atccagactc ggcaagacat cgacgaaacg aaatagggcc acgcggagcg
     4381 actcgtcctc aagcgaccac tcgatcatgc gcccgagcaa atccctcgtt tcgaataacg
     4441 tgggcttgtg ttcggcggct ttctggaaaa gcgtacgggc cagacgctga acttccggct
     4501 cgatgctctc gacggaaata tttttgtttg ccatgcgtta gaaaaagcgc agagacgtgg
     4561 ttgtaatgct ctctatcatc ataatacgat cgcgctggag aaaaagatcc tgaccccgtt
     4621 ttgcttatga cttttgaatt gaagaaagat tcctcgcttt cgccagactt tgtgaaaact
     4681 cgccgttcgt ccttcgacac gctcaggatg aacggctaag tagttgtcgt tgcagaacac
     4741 ctaagccgcg catgctgaac ttgtcgaagc atcagcggag ttttcacaca gtctgacgct
     4801 cggaatgaca aaattcggaa acttctcatc tcgaagcaaa gctgagaatc tttctcgact
     4861 gagcggcgtt tgcgagataa agaagcagcg gcatatatga cacgccccga cgaacaacat
     4921 ctagagaccc ttaccggcgc tgaagccgca gtggaaatgc ttaaagcgca cggcgtcgag
     4981 atccttttcg gcttgtgcgg cgacaccagt ctgccgttct acgatgcgtt ggcccgtctg
     5041 ccccacggga tgcggcacgt gctcacaagg gatgagcgct ccgcgtcgta tatggctgac
     5101 ggttacgcac gcgtttccgg caaagtcggc gtttgcgagg gtccgagcgg cggcggcgcg
     5161 acctacattc ttcccggtct cgctgaagcg aatgaatcat cggtgccgct cttgtgcatc
     5221 aattcggaca tttcggttgc cgcccgcggt cgctatacgc tcactgaatt ggaccagagc
     5281 gcgctcatgc ggccggtgac gaaatggaac agcgtgctca atcgcgccga agacttgccg
     5341 cgcatcttcc gcgccgcatt ccgacagatc acgaccggtc gtccgggcgc cgcacacatt
     5401 gcgctcccgt tcgacgtgca aaacggttcg gtcaatcgcg ctgaagtgtg gggggatgtt
     5461 cggtttggcc gttatccggc ggagcgaatt gcgccggatt cggaatctgt tgaatacgcc
     5521 acgcacttac tccgtgcggc aaaaaaaccg cttttgattt gcggcggcgg ggtaattttg
     5581 tctcaggcgc aagacgagct cgttgcgctg gccgagcaac tctcaatccc ggtcaccacg
     5641 accatcagcg gcaaaggttc cattgacgaa gagcacccgc tcgcggcggg ggtcgtcggt
     5701 tcgaatggtg gcaccccgga gacgcgcgcc atcgtcgaca acgccgatct ggtttttttt
     5761 attggctgcc gcgccggttc ggtaacaacc gagcgttggc gccacccaag acccggcgga
     5821 tgcaaagtga ttcatctcga tgtcgatccg gccgtcccgg gtgccaatta ccgcaccgac
     5881 gctgccttaa tctgcgacgc gaagctcgga ttgaagatgc tgcgcgaagc tctagcgaat
     5941 cagcgccgag tggttaacgg atcggacttg gaatctgcca aagaacggaa gttcgccaag
     6001 tttcaggtcc tcgccgaatc gctcgataag cctatcaaac cggagcgcct cgttgcggag
     6061 ctgacccgcg ttctcgatcg cgacgcaatt ctcgtcgcag atgccggtac gccctgtccc
     6121 tatttctcgg cttattatcc ggtgcgtcgt tcgggacgct gcttttccaa tcgcgcccac
     6181 ggtgcgctcg gctattctct ccccgccgct atcggcgccc acttcgctcg gccctcagtg
     6241 aagtgcgtct cagtgatggg cgacggcagt tttggcttta cctgcggcga gctcgagacc
     6301 gccgttcgct attgcctgcc gattactttt atcgtgatct caaacgcgag ctacggttgg
     6361 attaaagccg gacaaaaatc agggtacagt cagcgttatt tttcggtgga tttcgcagcc
     6421 acggatcatg ccgccgttgc ggccgctttc ggtgtaaaga gctggcgcgt aaccgagccg
     6481 acggagcttg gtaaacattt gaaagatgca ctcgcgtatg gtgggccaac tctcgtcgat
     6541 gttgtttgcc aatcgctcga ggaagccaaa gccccggtat ccgaatggat cgcctaagct
     6601 gtagacggcg cagcatgttg cgcccctacg ctgggcaaaa ttaattttcc agcctcacct
     6661 tcgttcccag atttgcacag tcctcgtcgg tcttgaggcc cgccgtgcgg attccgtgtg
     6721 cagcgagctc ctcatcacgc tcttgggtat cgccagtgac gccaaccgcg ccgagcagct
     6781 caccgccatt gccacggatc agcatg
//
LOCUS       DASULZ010000328         4494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_954864, whole genome shotgun sequence.
ACCESSION   DASULZ010000328 DASULZ010000000
VERSION     DASULZ010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954864"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /gene="lptC"
                     /locus_tag="VFY96_06845"
     CDS             <1..457
                     /gene="lptC"
                     /locus_tag="VFY96_06845"
                     /inference="COORDINATES: protein motif:HMM:NF018537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEX5606213.1"
                     /translation="RAKIEDGRKVWELFGDEASYFKEQKEALIKKPRFYYYDKKGEVA
                     ETKGNEALLYLNEKELERMELRGDVQVTFQGYILKSEEANYLPAKDQIVLPNRTTVTG
                     EGIELEGARMEVEIQDKKIRLVRAVKSKIEPEKLAKKKNSPSSTAPIGG"
     gene            487..1020
                     /gene="lptA"
                     /locus_tag="VFY96_06850"
     CDS             487..1020
                     /gene="lptA"
                     /locus_tag="VFY96_06850"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEX5606214.1"
                     /translation="MLLGSFLIFSAVPVGAAAPTKEQGKKGSTAFEFNKKDPIYITAD
                     WMEVDQTKNTITYKGRVVTIQSDMTMRSETLTAYYDPEMKQMKQIVAEGKVNATQGNR
                     VATGDKAVFDDKAKTVTLTGSPVMRQGNSQVSGVKVVYYMDQDRAVAEGDGKIRVQAT
                     VFPEELKNREQEDAKQK"
     gene            1017..1739
                     /gene="lptB"
                     /locus_tag="VFY96_06855"
     CDS             1017..1739
                     /gene="lptB"
                     /locus_tag="VFY96_06855"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEX5606215.1"
                     /translation="MSLLRAVSVVKCYGGRRVVDGINLEVKGSEVVGLLGPNGAGKTT
                     TFHMMVGLVQPDEGRVFMNDDDLTEAPIYQRARAGISYLPQESSVFRRLSVEDNLRAV
                     LQTLELSSDQQQERAEGLMKMLGIQHLAANKAYTLSGGERRRVEIARALVLSPFFLLL
                     DEPFTGIDPIAVAEIQKIVRRLIGSGIGILITDHNVRETLGICDRAYIVNEGTILEEG
                     TPETIAGSERARKFYLGEGFKF"
     gene            1742..3220
                     /gene="rpoN"
                     /locus_tag="VFY96_06860"
     CDS             1742..3220
                     /gene="rpoN"
                     /locus_tag="VFY96_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEX5606216.1"
                     /translation="MALEIRQVQKLTQQLVMTPQLQQAIKLLQLSRVELEEMVVKELQ
                     ENPALEEGPTEEPEGTAEQPRAEAPVATANDPADLVVNRELSAVDKIGTLDWQEYLDT
                     HSNSIHGSLTAEATSEDGDSPPSWENSLTKKTTLEDHLIWQLRLSKITERESTIGLHV
                     IQNLDENGYLVLSREDICSATETQPEEVEVVLRRIQFFDPVGVAARDLRECLLIQLEN
                     LKLSDSLAAKIVANYLSFLESKRYEKLAKELGVTIDEIAEAAHLIATLEPKPSRGFEQ
                     DEVRTVLPDVFVEKVGGEYVIFLNDDGVPRLRVNSLYRRLAGQEGAAEEQARQYLQEK
                     VRAAHWLIKSIQQRQQTLFRVTQSIFKFQLEFLDHGVSHLRPMVLRDVAEDIDMHEST
                     VSRATANKYVHTPQGLFELKFFFQSGLRGGNGEDVASESVKEKIRHIIAAEDPRKPYS
                     DQHIAAALSTDSIEIARRTVAKYREAMGILPSSKRRQPFFRK"
     gene            3246..3563
                     /gene="raiA"
                     /locus_tag="VFY96_06865"
     CDS             3246..3563
                     /gene="raiA"
                     /locus_tag="VFY96_06865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX5606217.1"
                     /translation="MSEIKVSVTFRHTQPTAALKRYAEQKIQRIGKYFDHPVEAHVVL
                     AVDGRERQLAELDLHTRGTTILAKEEHHDLYAAIDLAIDKIERQITKQKEKIKLERRK
                     SNA"
     gene            3560..4018
                     /locus_tag="VFY96_06870"
     CDS             3560..4018
                     /locus_tag="VFY96_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX5606218.1"
                     /translation="MKITDFLSRETIVPALASRDKSAALEEMAGYLGSTHHLDKNKVL
                     KVLLERERISTTAIGEGVAIPHGKLNGVERVVGVFARSLEGIDFASLDGAPTHLFFVL
                     VAPENAAADHLKALARISRLLKDETFRRRLMAGQSSQELYTTILEEDEKF"
     gene            4033..>4494
                     /locus_tag="VFY96_06875"
     CDS             4033..>4494
                     /locus_tag="VFY96_06875"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX5606219.1"
                     /translation="MAGPLDMIIITGLSGSGKSFAIRALEDNGFFCIDNIPALLIPKV
                     IDLSHGHEEEIRRIALGVDLRGGQFLQALPAVLSEMRDSGHRVQVLFFDAADEVLLRR
                     FSETRRPHPLAGKDSIQEGILRERKALEPIRALADKIIDTSDFNIHQLKREM"
BASE COUNT         1179 a         1096 c         1271 g          948 t
ORIGIN      
        1 tcgcgccaag atagaggacg gacgcaaagt gtgggaactg ttcggcgacg aggccagtta
       61 ttttaaggaa cagaaggaag cactgatcaa aaagccgcgc ttttattatt acgacaaaaa
      121 gggcgaagtt gctgagacca agggtaatga ggctcttctc tatctcaacg aaaaggaact
      181 cgagcgaatg gaactccgtg gcgatgttca ggtaacattt cagggctaca tactgaagtc
      241 agaggaggct aattacctgc ctgccaagga tcaaattgtt ctgccgaatc ggacgacggt
      301 cacaggagag gggatcgaac tagaaggtgc tcgcatggaa gtggagatac aagacaaaaa
      361 aattcgcctc gtgcgcgctg tgaagtcgaa gattgagccc gaaaaactgg cgaagaagaa
      421 gaatagccct agctcaacag cgccgatcgg gggataatga tgatgggtcg gaggcagaga
      481 actgcagtgc tgctggggtc gtttttgatc ttttcggccg tgccagtcgg cgccgcagcg
      541 ccgacgaaag aacaaggcaa aaaaggatcg acggcatttg aatttaacaa aaaggatcct
      601 atctatatca ctgcggactg gatggaagtg gatcagacaa agaacacgat cacatataag
      661 gggcgagtag tcaccattca atccgatatg accatgcgca gtgaaacgct cactgcttat
      721 tatgatcctg agatgaagca aatgaaacaa atcgtcgcgg aggggaaggt gaatgcgacg
      781 caaggcaatc gggttgctac cggcgacaag gctgtattcg atgacaaagc aaagactgta
      841 acattgacgg gtagcccggt gatgcgccag ggaaatagcc aagtgagcgg cgttaaagtg
      901 gtgtactata tggatcagga cagggcggta gcggagggtg acgggaagat cagagttcag
      961 gcgacagtgt ttccagagga gctgaaaaac cgggagcaag aggatgccaa gcagaaatga
     1021 gtctgctcag agcggtgtct gtggtgaaat gctacggcgg acggagggtc gttgacggga
     1081 tcaatttgga ggttaaaggc agcgaagttg tcgggttgct tggccccaat ggcgccggca
     1141 aaactaccac ctttcatatg atggtaggtt tggttcagcc ggatgagggt cgcgtgttta
     1201 tgaacgatga cgatttgacc gaagcgccga tctaccaacg ggccagagct ggcatcagtt
     1261 atttgcccca ggagtcgtca gtttttcggc gtctctctgt agaagataat ctccgcgccg
     1321 tgctgcaaac actcgaactg agcagcgatc agcaacagga gcgagccgag gggttgatga
     1381 agatgttagg tattcagcac ctcgcagcaa acaaggcata tacgctctcc ggaggcgaac
     1441 gccgcagggt ggaaattgct cgggcgttag tgttgtcgcc cttttttctt ctcctcgacg
     1501 aaccgtttac cggaatcgat ccgattgcgg tggctgagat tcagaaaatt gtgcgccggc
     1561 tgatcggtag cggcatcggc attcttataa ccgatcacaa cgttcgtgaa acgttgggaa
     1621 tctgcgatcg agcgtatatt gtaaacgagg gcacaatact cgaagagggt actcccgaaa
     1681 caatcgccgg gagcgaaaga gcgcgaaaat tttatttggg agaaggtttt aagttttaga
     1741 tatggctctt gagattcgcc aggttcaaaa gctgactcag cagctcgtaa tgactccgca
     1801 gctgcagcag gccatcaagc tgttgcaact gtcccgagtg gaattggaag aaatggtcgt
     1861 caaggagctt caggaaaacc ctgctcttga ggaaggaccc actgaagaac ctgagggtac
     1921 tgctgaacag ccaagggccg aggcgccagt ggccaccgca aacgacccag ccgatttggt
     1981 ggtcaaccgc gaattatccg cagtggacaa aatcggtacc ctcgactggc aagaatacct
     2041 cgacacccac tccaattcga tccatggctc tctcacggcg gaggcgacct ccgaagacgg
     2101 cgactcacca ccatcctggg aaaacagcct gacaaagaaa accaccctag aagaccatct
     2161 catttggcaa ctgcgtcttt caaaaatcac cgagcgggag tcgaccattg ggctgcatgt
     2221 cattcagaat ctcgacgaaa atggttatct cgtgcttagc cgggaagaca tttgtagcgc
     2281 gacagaaaca cagcctgagg aggtcgaagt cgtacttcgg cgcattcagt ttttcgatcc
     2341 tgtcggagtc gcggcgcgcg atcttcgcga atgcctgttg atccagctcg agaacctgaa
     2401 gctgagcgat agtctggcag ccaaaatcgt cgccaactat ctcagctttc tcgaatccaa
     2461 acgctatgaa aaattggcga aggaattggg cgtgacgatt gatgaaattg ccgaagcggc
     2521 gcatctcatc gccactcttg aaccaaagcc gtcgcgaggt ttcgagcaag atgaagtgcg
     2581 aactgttttg ccggatgtct ttgtcgagaa ggtcggcggc gaatacgtga tttttctgaa
     2641 cgatgatggc gtcccccggt tacgggtaaa ctccctgtat cgccgtcttg ccggtcagga
     2701 aggggcggcc gaggaacaag cgcgccaata cttgcaggag aaggtgcgcg ccgcccactg
     2761 gttgatcaag agcattcaac agcgacaaca gacattgttt agggtcaccc aaagcatctt
     2821 taaatttcaa ctggagtttc ttgatcatgg cgtcagccat ttgaggccca tggtgttacg
     2881 cgatgtcgcc gaggatatcg acatgcatga atcgacggtt agccgagcca ccgcgaacaa
     2941 gtacgttcat accccccaag gactttttga gctcaagttc ttctttcaga gcggcctcag
     3001 aggaggcaac ggcgaggacg tagcgagcga gagtgttaaa gaaaaaattc gccacatcat
     3061 cgcggcggaa gatccgcgca aaccctatag cgatcagcat attgccgcgg cgctatcgac
     3121 ggactcaatc gagattgcgc ggcgcaccgt ggcaaaatac cgcgaggcga tgggcattct
     3181 tccatcttca aagcgaagac agccgttctt ccgcaaataa atccctgagg agcgcttgcc
     3241 gaccgatgag cgagattaaa gtttcggtca cgtttcgtca tacccagcct accgcggcgc
     3301 tgaagcgcta tgcggaacaa aagatccagc gcatcgggaa gtatttcgac catcctgttg
     3361 aggctcatgt tgttctcgca gtggacggga gagagcgcca actggcggaa ctcgatcttc
     3421 atacccgcgg cacgacgatt cttgccaaag aagagcatca tgatttgtac gcggcgatcg
     3481 atttggccat tgacaaaatc gagcgccaga tcaccaagca aaaagaaaaa atcaagcttg
     3541 agcgaagaaa atccaacgcg tgaagatcac cgattttctc agccgagaaa ccatagttcc
     3601 cgcactggcc agccgcgaca aaagcgctgc gcttgaggag atggcggggt atttagggtc
     3661 gacacaccat cttgataaga acaaggtgct caaggttctg cttgaacggg aaagaatcag
     3721 tactacagcg atcggagaag gcgtggcaat cccgcatggc aaactcaacg gcgtcgagcg
     3781 agttgtgggg gtttttgcgc gcagcctcga gggaatcgat ttcgcgtcgc tggacggggc
     3841 tccaactcat ctgttctttg tcctggtcgc acctgaaaac gccgccgctg atcatttgaa
     3901 agctctggct cggatttcac gcctgctaaa ggacgagacg tttcgccgcc ggctgatggc
     3961 cggtcagtcg agccaagaac tttatacaac catattagaa gaggatgaga aattttgatg
     4021 tttgattgac cgatggccgg cccgttggac atgattatca ttaccggcct ttccggctcc
     4081 gggaaaagtt tcgcgattcg cgccctggaa gataacggtt ttttttgtat cgataatatt
     4141 cccgccctgc tgatccccaa ggttatcgac ctcagtcacg gccacgaaga ggaaatccga
     4201 cgtatcgctc tcggtgtcga tctgcgcggc ggccaatttc tccaagcgct tcccgcggtg
     4261 ctttccgaaa tgcgagactc cggtcaccgc gttcaggtct tgttttttga tgccgccgat
     4321 gaggtattgc tccgtcgatt tagcgaaaca cgccggcctc atccgctggc tggcaaggat
     4381 tccatacagg agggcatttt gcgcgaaaga aaggcgctcg aaccgatccg cgctttggcc
     4441 gacaaaatta tcgatacgag cgactttaat atccaccagc tcaaacgaga gatg
//
LOCUS       DASULZ010000329         3963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1461377, whole genome shotgun sequence.
ACCESSION   DASULZ010000329 DASULZ010000000
VERSION     DASULZ010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461377"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VFY96_06880"
     CDS             complement(<1..289)
                     /locus_tag="VFY96_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606220.1"
                     /translation="MSTRHLESRRARLAEDPSLALRLRLYRRLLPIPTALAVLVFFRP
                     NPSENPSVGTLIAAIGFVVCAIGQSLRLWAWGSNPRRGKKSAVRDRGAYALM"
     gene            complement(533..1333)
                     /locus_tag="VFY96_06885"
     CDS             complement(533..1333)
                     /locus_tag="VFY96_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5606221.1"
                     /translation="MRLIKFIAIVSLWLFFFGVLAIVHLWITVLRLPNRWKIVSRWIG
                     GFTWLMRRILNIKITVEGDAGQLEAGGYVIISNHFGYLDGIILGSLFPVIFVSKRKVK
                     SWPFVGQWTTLCGTVYINRQKKGLIPLAVNEISRRLKEGANILLFPEGAATNGERMLP
                     FQTAPLAAPLRSGATIIPVMLAYQSIEGQPVSAANRDSIYCYGGMDFAPHFVKLLGLR
                     QVEVRVTIQPKVDCSRYQDNSAGRKRLAEDCYDLILGGVGKRRLRASR"
     gene            complement(1689..2285)
                     /locus_tag="VFY96_06890"
     CDS             complement(1689..2285)
                     /locus_tag="VFY96_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006910913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEX5606222.1"
                     /translation="MKKFWKSAGSTFFGGLLVVVPLYLSVLLLLKAMQSLVGLVRPVA
                     MLLPAWFPAERALSLLLVIILCFLIGAAVRTPAGRAIRERIEKSLFERIPGYALLRSL
                     TQRIAGKDEERAWKPALVEIEEALVPAFIIEELDDGRFTVFVPSVPTPFAGAVYVLVP
                     ERVHPLDVPFTQALQSVSRWGSGSKELVAAMETETTLK"
     gene            complement(2590..>3963)
                     /locus_tag="VFY96_06895"
     CDS             complement(2590..>3963)
                     /locus_tag="VFY96_06895"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEX5606223.1"
                     /translation="HPGLSGFSIIRQGGPAFMARLAMADLAEKTLDGQYYIWDGDTTG
                     LILADHLLRAADRGVRVRLLIDDHYMSESRDNNIAALDVHPNIEIRFFNPVRNRRWRM
                     MSMLAEFGRVNHRMHNKLFVMDNAVGIVGGRNIADVYFGVQEDHNYRDLDVMSAGPIV
                     NEISASFDMFWNSDWALPVAAVVKARATEEDLRDLRKRLEEKLAATGYPYPVNEQLAG
                     LRDRLVQIRDNFVWAPGKVFVEQPSRVDNNEENVIALALRERASQVEHELSIESPYFV
                     LGDPTIERVRQLTARGVKVRVLTNSAASHDVLPALAGYVNTRKKVLNAGVELYELRPD
                     SDMPRTWSVLAAKSQAALHAKCLVFDRKSVFIGSFNLDPRSTALNTEIGVMIDSPEIA
                     GQVGELMDEGVAPGSAFRVTLDKNNLVWSAEKNGEKVEYDTDPETSVFYRFLVGILGM
                     LPIEDQL"
BASE COUNT          923 a         1175 c          997 g          868 t
ORIGIN      
        1 gcatcagcgc gtaagctcct cgatcgcgaa ccgcggattt ttttcctctt cgcggattgc
       61 tcccccaggc ccagagccgg agcgattggc caatcgcgca gacaacaaaa ccgatggccg
      121 ctatcagcgt cccgacagag ggattttccg agggatttgg ccgaaaaaag accagcacag
      181 ccagcgcagt gggaataggt agaagacgcc gatacaagcg tagcctcagg gccaagctgg
      241 gatcttctgc caaacgagcg cgccgagact cgagatgtcg ggtcgacacg ccaatttcct
      301 ccgtttgtct aaattgagaa actttttcgc tcataaacga aaatcactgc gcaggtacta
      361 cgccaaggat atctgccaaa gaaagtccca cgttccctcg cagctctctc tgacacctat
      421 ttaaaacctg cttggcaaag ctctaataga tagcttcatc tgctactccc gatagtatcg
      481 gatcagtcca ctttgctatc ctagcaggtc caagacttgg aacatgctcg ccttatcttg
      541 atgcgcgtag ccttctcttg cccacgcctc ccaaaatcaa gtcgtaacaa tcctccgcca
      601 gtctcttcct gcctgccgag ttgtcttgat atcgagaaca atcgaccttc ggttggatcg
      661 tcaccctcac ttcgacctgg cgtaacccga ggagcttcac aaagtggggg gcaaaatcca
      721 tacccccata acagtagatc gagtcccggt tggcggccga gaccggttgt ccctcaatgc
      781 tttggtaagc cagcatcact ggaataatcg tcgccccgct tcgcagcgga gcggccaacg
      841 gagcggtctg gaacggcaac atccgctcgc cgttggttgc agctccctcg ggaaacagca
      901 aaatattggc tccctctttt agtctgcggc tgatctcgtt aaccgccagc ggaatgagac
      961 ccttcttttg ccgattgata tagacagtcc cgcaaagtgt cgtccactga ccgacgaacg
     1021 gccagctttt cactttcctt tttgagacga aaattaccgg gaaaaggctt ccaagaatga
     1081 tcccgtcgag gtaaccgaag tggttgctga taatcacata accaccggct tcaagctgcc
     1141 ctgcgtcgcc ctccaccgtt attttaatat ttagaatcct cctcattagc cacgtaaatc
     1201 ctcctatcca gcggctgaca attttccagc ggttgggaag tcttagcacg gtgatccaca
     1261 agtggacgat tgccaagacg ccgaagaaaa acagccacaa ggaaacgata gcgatgaact
     1321 taatcaaccg catgacccac ctgatgtctc aagcgcccaa tactagtcac cgctcgactt
     1381 gtgaatgtcc agcgccacta aaaaaacgtg ctgaataatt ctgaccccta acaagcatca
     1441 acctcgctca tgccacggac tcaaagttgt cgaaaagatg atccattact ccatcaaaaa
     1501 gccggtgagc ttgtatattg gactttggtc caatgccttt ctatcgcggc cattaggcac
     1561 gaaaacagca cagctgatgc gacagattcc ggagcagcgt aaacgttgaa cttctcttta
     1621 cctcgaaaaa tagcggcgaa gaacttcttc aagcctcgcc actaacgctc tcatgaaaat
     1681 cttcgttgct atttgagagt ggtctcggtt tccatcgctg ccacaagctc ttttgaacct
     1741 gatccccagc gggagacgga ctggagcgct tgtgtgaagg ggacgtccaa agggtgtacc
     1801 cgttcgggaa cgagaacata gaccgctccg gcaaacggtg tcggcaccga cggcacaaaa
     1861 accgtgaaac gcccgtcatc caattcttcg ataatgaacg cggggacaag cgcctcctca
     1921 atctccacca gagccggctt ccacgcgcgc tcctcgtctt ttccggctat gcgttgcgtc
     1981 aggctccgaa gcagtgcgta acctgggatt ctttcaaaaa gagatttctc gatacgctct
     2041 cggattgctc gccctgccgg cgtgcgaacc gcagcaccga tgagaaaaca taggattatc
     2101 acgaggagaa gtgacagtgc ccgttcagcc gggaaccatg ccgggagcag catggcaacg
     2161 ggccgcacca aaccgacaag cgattgcatc gccttgagca gcagcaacac ggaaagataa
     2221 agcggcacga cgacgagcaa gccgccaaag aacgtgctcc ccgcggattt ccagaacttt
     2281 ttcatacacc gccctctctt gtttagcttc ttcggcggct cgctgctccg actctccggt
     2341 tcccgcgccc atcctctcgc ccaggtcgga taggttatcg atggcttcct gcacgaaggt
     2401 gctgccaatg gcggcgatca gtggcggaaa tcgaacgttt cgttccagct tatttagtac
     2461 cgtgctgcac agcagaacat tgcgcagctc gctgctccag ctcgtggggt ttgcgccatc
     2521 ttttcgccga gatcctccag attgttcatc gtctctttga taaacgcccc accagtgccc
     2581 cagcgtcctc tatagttggt cctcaattgg aagcatgccg agaatgccga ccagaaaccg
     2641 gtaaaagacg cttgtttccg gatctgtgtc gtactccact ttctccccgt tcttctcggc
     2701 gctccagacc agattgttct tgtcgagcgt gacgcgaaag gcactgccgg gggcgacccc
     2761 ttcgtccatc aattctccca cctggccggc aatctccgga ctgtcgatca tcacaccgat
     2821 ctcggtgttg agggccgtcg atcgggggtc gagattgaaa ctgccgatga acacggactt
     2881 gcggtcgaag acaaggcact tggcatgcaa ggcagcttgc gatttagccg ccaacaccga
     2941 ccaggtccgt ggcatatcgc tatccggacg caactcgtat agctcgacgc cggcattgag
     3001 cacttttttg cgtgtattga catagccggc aagcgccggg agcacatcat gggaggccgc
     3061 ggaattggtt aatacacgca ccttgacacc tcgcgctgtg agttgtcgca ccctttcgat
     3121 cgtcgggtcg ccaaggacga aatacggcga ctcgatcgac agttcgtgct ccacctgact
     3181 ggcccgttcg cggagtgcca aagcgatgac gttctcctcg ttgttgtcaa ccctggaagg
     3241 ttgctcgacg aaaactttgc ccggggccca gacgaaattg tcgcggattt ggaccaagcg
     3301 gtcgcgcagg ccagcaagct gttcgttcac gggataggga taacccgtgg cagcaagttt
     3361 ttcctctagc cgtttcctta agtcccgcaa atcctcttcg gtggcgcgcg ctttcacgac
     3421 cgcagcgacg ggtagggccc agtcgctgtt ccaaaacata tcgaagctcg ccgatatctc
     3481 gttgacgatc ggaccggcgc tcataacatc gagatcgcga tagttatggt cctcctgaac
     3541 gccgaaatag acatccgcga tgttgcgtcc gccgacaatt cccacggcgt tgtccatgac
     3601 gaacagcttg ttatgcatgc gatgattgac gcggccaaac tcggccaaca tactcatcat
     3661 gcgccaacgc cggttcctga ccgggttaaa gaaacggatc tcgatattgg ggtggacgtc
     3721 gagcgctgca atgttgttgt ctctgctttc cgacatgtaa tgatcatcga tcaacaaacg
     3781 cacacggacg ccgcgatccg cggcacgcag cagatggtcg gccaagatca ggccagtggt
     3841 gtcgccatcc cagatgtaat actgcccatc gagcgtcttt tcggccaagt cggccatcgc
     3901 cagccgggcc ataaacgctg gaccgccttg ccgaataata gagaagccgg atagtcccgg
     3961 atg
//
LOCUS       DASULZ010000330         3890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1361269, whole genome shotgun sequence.
ACCESSION   DASULZ010000330 DASULZ010000000
VERSION     DASULZ010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361269"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="VFY96_06900"
     CDS             <1..812
                     /locus_tag="VFY96_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5606224.1"
                     /translation="GTLNLLEALRAADEPPPLVFTSTNKVYGGLEDIALNSERGIYYP
                     MDMAVRANGIDELRPLDLHSPYGCSKGAADQYIIDYARTFGLPAVVFRMSCIYGPHQM
                     GNEDQGWVAHFLIRALEEQTITLYGDGKQVRDVLYVDDLVDAFLLARAEMPRIAGQAF
                     NIGGGPEQAISLLELIDMIARLHGHQPQYEFQDWRPGDQRYYVSDTRKFRNATGWAPK
                     VRPHAGTEKLYRWLRDSGSLGQSRSEPVRPRQPVVYRRAPSRNGVSAAERH"
     gene            817..1839
                     /locus_tag="VFY96_06905"
     CDS             817..1839
                     /locus_tag="VFY96_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018480104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5606225.1"
                     /translation="MQSAPQLQMTGRKPPHVRAAVTTMRAARLVAPGRVEVNEVPVPR
                     PRSTEVRIRVAGCGICGSNLALWQGRPWFSYPFEPGEGGHEGWGEVDALGEAVHDFRL
                     GDRVAFISNHAFAEYDLADSAQIVPAPPHSDVFPGEAIGCAVNIIRRSALETGQSVVV
                     IGIGFLGALLVQLAARVGARVIAVSRRRSALETARRCGADATIVFDRAETVAAQLRDM
                     TGEATSERVIEAAGAQASLDLATALTGEYGRLIIAGYHQDGPRQVNMQLWNWRAIDVI
                     NAHERDAAISTQGIREAAALIDRGALDPSILYTHRFLIDELSEAFKLLEGRPEGFLKA
                     WIRTAA"
     gene            1917..3041
                     /locus_tag="VFY96_06910"
     CDS             1917..3041
                     /locus_tag="VFY96_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX5606226.1"
                     /translation="MATGKKVRVAIIGVGNCASSLVQGVEFYKNAPEDEFVPGLMHVK
                     LGDYHVRDVEFSAAFDIDINKVGKDLAEAIFTVPNNTYKFADVAPLGVPVRRGMTHDS
                     VGKYLAEVIRKSPGATDDIAGILRATRTDVVVSYLPVGSEMATKWYVEQVLEAGCALV
                     NCIPVFIASQPYWRKRFKERGLPIIGDDVKSQLGATIAHRVMAHLFKDRGVRLDRTYQ
                     LNFGGNTDFLNMLERERLESKKISKTNSVLSQIDYSLTPANAHVGPSDYVPWLEDRKW
                     CYIRMEGTTFGNVPLNCEMKLEVWDSPNSAGVVIDAVRCAKLALDRGLGGALVGPSSY
                     LMKTPPQQFSDHEARARIDAFIAGEEYGSSKVAARLAKAR"
     gene            3038..>3890
                     /locus_tag="VFY96_06915"
     CDS             3038..>3890
                     /locus_tag="VFY96_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5606227.1"
                     /translation="MKLVIFGLTISSSWGNGHATIWRGLCRALSRRGTRIVFFERDVP
                     YYSAHRDLYELPGGQLELYQSWETVLPRAERQLADADVAIVSSYCPDGIAASELVLGS
                     RAYRVFYDLDTPVTLEHLDRGESVSYIGARGLADFDLVLSYTGGEALKKLQRRLGARR
                     VCALYGSVDPSLHSPCTPVGDFRADLSYIATYAADRQQALETLFLAPARRSGARRFLI
                     AGAQYPADFPWTKNIFFVRHVPPSAHAALYCSSRLTLNLTRAAMRRMGYCPSGRLFEA
                     AACATPVI"
BASE COUNT          756 a         1141 c         1184 g          809 t
ORIGIN      
        1 tgggaacgct gaacctgctc gaggcattgc gcgcggccga cgaaccgccg ccgctcgtat
       61 tcacttcgac caataaggtt tatggcggcc ttgaagacat tgcgcttaac agcgagcgcg
      121 gtatttatta tccgatggac atggccgtcc gtgctaacgg aatcgatgag ctccggccgc
      181 tcgatctaca cagcccttac ggctgttcca agggagccgc agaccagtac atcatcgact
      241 acgctcggac gtttggcctg ccggcggtag tatttcgtat gagctgcatt tacggacctc
      301 atcagatggg taatgaagat cagggctggg tcgcgcactt tctgattcgc gcccttgaag
      361 aacagacgat tacgctctac ggcgatggca agcaagtgcg tgatgttctc tacgtcgatg
      421 atctggtcga cgcgtttttg ctcgcacgcg cggaaatgcc gcggatcgcc gggcaggctt
      481 tcaatatcgg cggcggtccg gaacaggcga tcagcctgct tgagctaatc gatatgattg
      541 cgcgcctgca cggccatcag ccgcagtatg aatttcagga ttggcggcca ggcgaccaac
      601 gctactatgt ttccgacaca cgcaaattca ggaatgcaac cggatgggcg cccaaggtcc
      661 gtccccacgc tggaacagag aaactgtatc gttggctgcg cgattccggg agtctcggcc
      721 aaagccggag cgagccggtg cgaccgcgcc aaccggtggt ttatcgacgc gccccttcgc
      781 gtaacggggt ttccgcagcg gagcggcatt gaggcaatgc agtccgctcc gcaactacag
      841 atgacgggcc gcaaaccgcc ccatgttcgg gcggcggtta cgaccatgcg cgccgcgcgc
      901 cttgtggcgc ccggccgagt cgaggtgaac gaggttcccg tacctcgccc gaggtcaacg
      961 gaggttcgta ttcgagtcgc aggctgtggt atctgcggct cgaatctggc tctgtggcag
     1021 ggtcggccct ggttttcgta tccgtttgag cccggcgaag gtggtcatga aggctggggc
     1081 gaagtcgacg cgctgggtga agcggttcac gattttcgct tgggcgaccg cgtcgctttt
     1141 atttccaacc acgcatttgc cgaatacgat ttggcggata gcgcgcaaat cgttccggcg
     1201 cctcctcaca gcgatgtttt tcccggtgag gcgatcggct gcgcggtcaa catcatcagg
     1261 cgaagtgctc tcgaaaccgg ccaaagcgtg gtggtgatcg gcattggatt tctcggtgca
     1321 ttgttggtgc agctagccgc gcgagtaggc gcgcgcgtga tcgcggtctc ccggcgtcgc
     1381 tctgccctcg agactgcgcg acgctgtggt gcggacgcga caatcgtgtt cgatcgggcc
     1441 gaaacagtgg ctgcccaact gcgagatatg accggagaag cgacgtccga gcgggtcatc
     1501 gaagcagcag gcgcacaggc gagtctcgat ctggccaccg cactcaccgg tgaatacggc
     1561 cgtctgatca ttgccggcta tcatcaggac ggtccccgcc aggtgaacat gcagctttgg
     1621 aactggcgcg cgattgacgt catcaatgcg cacgagcgcg atgcggcaat ctcaacccaa
     1681 ggcatacgcg aagcagccgc attgatcgat cgtggcgcgc tcgatccatc catcttatat
     1741 acgcataggt ttctcatcga cgagctctct gaggctttca aattgttgga agggcgtccc
     1801 gaaggttttt tgaaggcttg gatccgcacc gcagcgtgag cgccggggca gcggttgaag
     1861 gggaaagagc ttcagcggat gatcgaatca catcggcgcg aggaagggag caaggaatgg
     1921 caacaggcaa gaaggtcagg gtggcaatca tcggcgtcgg caactgcgcc tcctctttgg
     1981 tgcagggcgt tgagttctac aaaaacgcac cggaagacga attcgttccc ggcctgatgc
     2041 acgtcaaact cggcgattac catgtgcgcg acgtcgagtt cagcgcggca ttcgatatcg
     2101 acatcaacaa agtcggcaaa gatctggctg aagcgatttt cactgtgccg aacaacacct
     2161 acaaattcgc cgacgtcgcg ccgctcggtg ttcccgtcag gcgcggcatg acgcatgaca
     2221 gcgtgggtaa atacctggcg gaggtgatcc gcaaatcccc cggcgcgacc gacgacatcg
     2281 cgggcattct gcgcgccaca cgcaccgacg tggtggtttc gtatcttccg gtgggctccg
     2341 agatggccac caaatggtac gtcgaacagg tgctcgaagc gggttgtgcg ctggtcaact
     2401 gcattccggt attcatcgct tcgcaaccgt actggcgcaa gcgcttcaaa gaacgtgggc
     2461 tgccgatcat cggtgacgac gtgaaatctc agctcggggc caccattgca catcgggtca
     2521 tggctcacct gttcaaggac cggggcgtgc gtctcgatcg gacttatcag ctgaacttcg
     2581 gaggcaacac ggatttcctt aatatgctcg aaagggagcg gctggagtcg aaaaaaattt
     2641 ccaaaaccaa ttcggtgttg agtcaaatcg actattctct gacgcctgcg aacgcccacg
     2701 tcggtcccag cgactatgtg ccttggctcg aagatcgaaa gtggtgctac atccgcatgg
     2761 aaggaacaac cttcggcaac gtgccgctca actgtgagat gaagctcgag gtctgggact
     2821 cgcccaattc agccggcgtg gtgatcgacg cggtgcgctg cgccaaactg gccctcgatc
     2881 gcggcctcgg cggcgcgctc gtcggaccgt cgagctatct gatgaaaacg ccacctcagc
     2941 aattttcgga tcacgaggcg cgggcgcgta tcgatgcctt catcgccggt gaagagtacg
     3001 gcagcagcaa ggtcgccgcg cgcctagcta aggcgcgatg aagctggtta ttttcggact
     3061 gacgatcagc tcttcgtggg gcaatggtca cgccacaatc tggcgcgggc tttgccgtgc
     3121 gctcagccga cgcggcactc gtatcgtatt tttcgagcgc gacgtcccct attattctgc
     3181 gcatcgcgat ctttacgaat tgccgggtgg gcagctcgag ctctaccaaa gttgggaaac
     3241 agtactgccc cgggccgagc ggcaactagc ggatgccgat gtcgccatcg tcagctcgta
     3301 ttgtccggac ggaatcgccg cctctgagct tgtgctcgga tcccgcgcct accgcgtttt
     3361 ttatgacttg gatacgccgg tgacgcttga acacttggat cgcggtgaat ccgtcagcta
     3421 catcggcgcg cgcggcttgg cagatttcga tttggtgctg agctacaccg gcggcgaggc
     3481 cttgaaaaaa ctgcaacgcc gtttgggtgc gcgccgcgtc tgtgcactct acggcagcgt
     3541 cgatccctcg ctgcactcgc cttgcacacc ggttggcgat tttcgcgccg atctttccta
     3601 tatcgccacc tacgctgcag atcggcagca ggcgttggaa acgctctttc tcgcgccggc
     3661 gcgtcgctcc ggcgcgcgcc gtttcctcat cgccggagcg caataccccg ccgatttccc
     3721 ttggaccaag aacatttttt tcgtgcgcca tgtgccgccg agcgcacatg cagcgctcta
     3781 ttgctcgtca cgtctcacct tgaacctgac gcgggccgca atgcggcgca tgggatattg
     3841 tccctccggc cgtctattcg aagcggcggc ttgcgccacg cctgtgatca
//
LOCUS       DASULZ010000331         3511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_549265, whole genome shotgun sequence.
ACCESSION   DASULZ010000331 DASULZ010000000
VERSION     DASULZ010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549265"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="VFY96_06920"
     CDS             complement(<1..1094)
                     /locus_tag="VFY96_06920"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5606228.1"
                     /translation="MNIFDALERSKTRYPDKDVIWCRGVAMTYAQLYHQVCCLSSALQ
                     EENLQKGERVALFLPNRPEFLISYFATVRLGAIAVSLNVMLKRDEVEFILNDCQARIL
                     ITCDRLLDQLPHSHETPALKSIVCVDEPHHTGVIGIHKLLARGRASHPTANSAQDEGA
                     AILYTSGTTGKPKGALLSHGNLVSNVASTVFHTKMSSNDRLICFLPLFHCFGQNFIMN
                     AAIACGATLVLHERFQLDEMLESIKTNRISMFFGVPAVYLRLLSLADIEERFRSLRYC
                     FTAAAPMPLAVARQWRERLGRPIYEGYGLTETSPFASYNHDIDYREGSVGSPIENVEM
                     KIVDPDGLELPVGEQGEIAIKGPNVMLGYFR"
     gene            complement(1091..2692)
                     /locus_tag="VFY96_06925"
     CDS             complement(1091..2692)
                     /locus_tag="VFY96_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HEX5606229.1"
                     /translation="MDMDPSLVEITGDGLTIDRVASVARQDSRVELSSNRAVVNKISS
                     SRQLLEKKLAAGEVIYGVNTGFGGNVRFLIPDSDLSFHQVNLLRSLSCGVGQPLPADA
                     VRAAMLLRANALAKGLSGVRREIIDRLVDLLNHRITPVVPRYGSVGASGDLIPSAYIG
                     RVLLGEGEVFYQGGRVPAAEALRHANLAPSELTAKEGLALVNGTTVMTGIAALVIYDG
                     AYLARLVLACASLAIEGLNATADPFSEVIHRAKNHPGQVEAAAICRKLLQDSRYIRNL
                     DEIRTRVSETRKASEAKTIRSDEAIQTPYSLRCVPQGIGPVLEALSAHQVILEREMNS
                     ANDNPLIDPVEGRVYHTGNFYGGHVARALDSWKIDLATLANWLHSIMAMLIDDRFSSG
                     LPPNLTRKPGLSSGFKGMQLCLTSLVCAIRHLSNPSMVHSLPTEQYNQDMVSLGTLAA
                     LTAMEMTAMARDAAAISLLALCQAIDLRGGGENLGIGTRAVFETVRKQVAILENDRPL
                     DSDLARTSELIRARLLPLPELENGL"
     gene            complement(2756..>3511)
                     /locus_tag="VFY96_06930"
     CDS             complement(2756..>3511)
                     /locus_tag="VFY96_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5606230.1"
                     /translation="VSRVAQKAFKEIRLNTKVLKMATSGRQIKVTTEVNKEQRDELYD
                     RVLVSVGRVPNLADLGLENTKVTKDDKGFIQCNAQLQTEDPNIYAVGDATGGTLLAHR
                     AFRQGKIAVEAILGEASAFENVVIPAVVFTDPEVAWCGLTETEAQQKGIEVEVAKFPW
                     GASGRALTFDRPDGLTKLVIEPETERILGVGIVGVGAGELIGEGVLAVEMGATVRDLA
                     DSVHPHPTLSETLMEAAESFYGTATHTLSRRSS"
BASE COUNT          787 a          958 c          973 g          793 t
ORIGIN      
        1 cgaaagtagc ccagcatcac gttcggtcct ttaatcgcaa tttccccttg ctcgccaacc
       61 ggcagctcta gtccgtcggg atcgacaatt ttcatctcga cgttctctat gggagacccg
      121 acgctgccct cacgatagtc aatatcatga ttataactgg caaagggcga ggtttccgta
      181 aggccatagc cttcataaat gggccgacca agccgttccc gccactggcg cgcgaccgct
      241 aacggcatcg gcgccgcggc agtgaagcag tagcgaagcg aacgaaaacg ttcttcaatg
      301 tcggccaatg acagaaggcg tagataaacc gccggaacgc caaaaaacat cgagattcgg
      361 ttggtcttta tagattcgag catctcgtca agttgaaacc gctcatgaag gaccaatgtc
      421 gccccgcacg cgatggctgc attcattata aaattttgac cgaaacagtg aaaaagcggc
      481 aaaaaacaga tgagccggtc attggaagac attttcgtgt ggaaaacggt tgaggcgaca
      541 ttcgagacga ggtttccatg cgaaagcagg gcgcccttcg gttttcccgt agttcccgaa
      601 gtgtaaagga tcgccgcgcc ttcgtcctga gctgagttgg cagtcgggtg agaggctctg
      661 ccgcgggcga gcagtttatg tataccaatg acgccggtgt gatgaggctc atcgacgcag
      721 acaatgcttt taagcgccgg agtttcgtga gaatgcggca gttgatccag caaccggtca
      781 caggtgatca ggatccgagc ttggcaatca ttgagaatga actctacttc atcacgtttg
      841 agcatgacat tgagggaaac cgcaatggcg ccgaggcgaa cggtagcaaa atacgatatc
      901 aaaaactcgg gccggttggg aagaaagagc gctactcgct cccctttctg cagattttct
      961 tcttgcagcg ccgaactcaa acaacaaacc tggtgataga gttgcgcgta tgtcatcgca
     1021 acgcctcgac accatatgac atctttatct ggatagcggg ttttgctccg ttccaaagca
     1081 tcgaagatgt tcataagccg ttctccagtt caggaagcgg cagcagcctc gctcgaatca
     1141 actcggatgt acgggcgaga tcgctgtcca gcggccgatc gttttctaaa atcgccactt
     1201 gttttctaac cgtttcaaag acggctctgg tgccgatgcc gagattttcg ccgccgcccc
     1261 ggaggtcgat ggcctggcaa agcgcaagca gcgagatggc ggcggcatct cgcgccatcg
     1321 ccgtcatttc catagccgta agcgctgcaa gcgtgccgag actgaccata tcctgattgt
     1381 actgttctgt tggcaagcta tggaccatac ttggattgga caaatgacgt atggcgcaaa
     1441 cgaggctggt cagacacaac tgcatccctt taaaacccga cgatagtccc ggttttcgcg
     1501 ttagattggg tggcaggccg ctactgaatc gatcatcgat aagcatagcc atgatcgagt
     1561 gaagccagtt ggccaaagtg gccagatcga ttttccaact gtcgagcgcg cgtgccacat
     1621 ggcccccgta gaaattgccg gtgtgataga cgcgtccttc taccggatcg atcagcgggt
     1681 tatcgttggc ggagttcatc tcgcgttcca agatgacctg atgggcgctc aaagcctcga
     1741 gcaccggacc gattccctgc ggtacgcaac gcaatgaata gggagtctga atggcttcat
     1801 cggagcggat cgtttttgct tcagatgcct tgcgcgtctc actgaccctg gtgcgaattt
     1861 cgtcgagatt tcgaatgtaa cgactgtcct gcagtagctt gcggcagatc gccgccgctt
     1921 cgacttgacc gggatgattc ttggcccggt ggatcacttc ggaaaacggg tcggcggtgg
     1981 cgtttagccc ttcgatggct aggcttgcgc acgccagcac aaggcgtgcg aggtaggcgc
     2041 cgtcatagat gaccagcgca gctatgccgg tcatcacggt ggtgccgttg acgagcgcta
     2101 gtccctcttt cgccgtgagt tcggagggcg cgaggtttgc gtgccgcagc gcttcagcgg
     2161 caggaactct tccgccctgg tagaaaactt caccttcgcc gaggaggaca cggccaatat
     2221 aagcggatgg aatcaagtcg ccgctggcgc ccacggaacc gtagcgcggt accacaggtg
     2281 taatccggtg attgaggaga tcgaccaatc ggtcgatgat ttcacgtctt acgcccgaaa
     2341 gccccttagc gagagcattc gctctgagga gcatcgcggc ccgaaccgca tccgccggca
     2401 gcggctgacc aacgccgcag ctgagagaac gaagaagatt tacttgatga aagcttagat
     2461 cgctatcggg aatgaggaaa cgcacgttgc cgccgaaccc cgtgtttacg ccatagatca
     2521 cctctccggc cgctaatttt ttctccaaca actgccggga ggaagaaatt ttatttacga
     2581 cggcgcgatt tgatgacagc tcgacgcggc tatcctggcg ggctaccgaa gcgaccctgt
     2641 cgatcgtgag gccgtctccg gtgatttcga cgagagaggg atccatgtcc atgaaaaccc
     2701 cctttaacat ctcgctcgac tatagcatta tatcgggcgg aaaaatagga atgcttcagg
     2761 aagagcggcg cgatagtgta tgtgttgctg ttccgtagaa actctcggca gcttccatca
     2821 atgtttccga gagcgtcggg tgggggtgca cagaatcggc gaggtcgcga accgtagctc
     2881 ccatttcgac ggcgagtacg ccctcgccga tcagttcgcc tgcgccaact ccgacaatcc
     2941 ccacgcccag aatccgttcc gtttcaggct cgatcaccaa ttttgttagg ccgtccggtc
     3001 gatcgaacgt cagggctcgg ccggatgcgc cccatgggaa tttcgcgact tcaacctcga
     3061 tgcccttttg ctgcgcttcg gtttccgtaa ggccgcacca ggcaacttct ggatcggtaa
     3121 aaaccaccgc cggaatgact acgttttcaa acgcgctagc ttctcccaga attgcttcga
     3181 cggctatttt gccttgcctg aaggcgcgat gagcgaggag agtgccgccg gtggcatccc
     3241 cgactgcata gatattcgga tcctcggtct gcagttgcgc gttgcactga ataaaacctt
     3301 tgtcgtcttt ggtgaccttg gtgttttcaa gtccgagatc agcgaggttc ggcacccgtc
     3361 cgacactgac gaggacgcga tcatagagtt cgtcgcgctg ctctttattg acctcggtcg
     3421 tgactttaat ttgcctacca gaagtcgcca tttttagaac tttggtgttc agacgaattt
     3481 ccttgaatgc cttttgcgcg acgcggctga c
//
LOCUS       DASULZ010000332         2768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_751536, whole genome shotgun sequence.
ACCESSION   DASULZ010000332 DASULZ010000000
VERSION     DASULZ010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751536"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..2168
                     /locus_tag="VFY96_06935"
     CDS             189..2168
                     /locus_tag="VFY96_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008811317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase flavoprotein subunit"
                     /protein_id="HEX5606231.1"
                     /translation="MKIIYTDVLVIGGGLAGLRIGIGAKRRGHDVIILSLVPPKRSHS
                     AAAQGGMQASLGNVIKGEGDNEDVHFEDTVRGSDWGADQDVVRMFVNTAPKAVRELAA
                     WGLPWSRVTRGDRDVIINGQKVTITERDEAHGLIAQRAFGGTNKWRTCYVSDGTGHAM
                     LTTMSDQAIANRIPVHERAEAIALIHDGARCYGAIVRNLVTGELVAYVAKATAITGGG
                     AGRLYRVTTNAVISEGIPTAIALETGVATLGNMEAVQFHPTPAFPAGILLTEGARGDG
                     GLLKDVDGHRFMPDYEPEKKELASRDVVSRRMEEHIAKGKGVKSRFGEHLWLDITLLG
                     EHHIKHNLREVYEICHYFLGVDPVKDWVPVRPAQHYTMGGVRTNYTGESPTLKGLFSA
                     GEAACWDMHGFNRLGGNSVAETVAAGMIVGEFIADFCERSDSGANIPTSLIQETFQHE
                     QAKIDALMEGRGTENAAAIMAEMQSVMTAKVGIFRTGRVLEEAVHELQQLLLRSRNIG
                     LRYKARGANPELVTAYRVRKMLKMALCVAYGAYARTESRGAHFREDFPRRNDAEWLNR
                     TLATWKSENDTLPSLTYEQLDVMKMELPPGWRGYGAKDHIDHPDTARRLAEIEALKQS
                     RDGVDRFAFQAKLMPYQHLLPETFRGRNERIDERL"
     gene            2329..>2768
                     /locus_tag="VFY96_06940"
     CDS             2329..>2768
                     /locus_tag="VFY96_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX5606232.1"
                     /translation="MAENRIERDTMGEIAVPADRYWGAQTQRSLNYFQAGAGRDTMPR
                     ELIKAFGILKKAAAMANVELKLLDKERGDLIIQVADEVISGKLDDHFPLVVWQTGSGT
                     QTNMNVNEVISNRAIEISGGVMGSKKPIHPNDDVNKAQSSNDTFP"
BASE COUNT          626 a          767 c          853 g          522 t
ORIGIN      
        1 atcaacgcta ttttggttgt gctgaataac atgagtttaa gctgttcaag ctgctcaagc
       61 cctctccgta gtcctccccc gcgtcgcggg ggaggaaaga ggtgggggtt tgaacggagc
      121 gaagcgattg aatggcttga acggttggaa cccattgggg cacacgccaa gcgagagaga
      181 gctaagccat gaagattatc tacacggacg ttctggtcat tggcggtggg cttgccggtt
      241 tgcgcatcgg catcggcgcg aagcggcgcg gccacgatgt gattattttg tcgctcgtgc
      301 cgccgaagcg ttcccattca gcggccgcgc aaggcgggat gcaggcgagc cttggcaacg
      361 ttatcaaggg cgaaggcgat aatgaagatg tgcacttcga agataccgtg cgcggcagcg
      421 actggggcgc cgaccaggac gttgtgagga tgttcgtcaa caccgcgccc aaagcggtgc
      481 gtgaactggc cgcgtggggc ctgccttgga gccgcgtgac ccggggtgac cgcgacgtga
      541 tcatcaacgg acaaaaggtc acgatcaccg agcgcgacga agcccacggc ctgatcgcgc
      601 agcgagcctt cggcgggacc aacaagtggc gcacctgtta tgtgtccgac ggcaccggcc
      661 acgccatgct cactacgatg agcgatcagg cgatcgccaa ccgcattccg gtgcacgagc
      721 gcgcggaagc gatcgctttg atccacgacg gcgcacgttg ttacggcgcg atcgtgcgaa
      781 atcttgttac cggagagttg gtcgcttacg tcgccaaggc cacggcgatc accggcggcg
      841 gcgccgggcg gctttaccga gtcaccacca acgcagtaat ctccgaaggc attcctacag
      901 ccatcgcgtt ggagacgggc gttgctactc tcggcaacat ggaagcggtg cagtttcatc
      961 cgacaccggc gtttcccgcc ggcattcttt tgaccgaggg agcgcgcggt gacggcggat
     1021 tgctgaagga cgtagacggc catcgcttta tgcccgacta cgagccggag aaaaaagaat
     1081 tggcctcgcg cgacgtcgtc tcgcggcgca tggaagagca catcgccaag ggtaaaggcg
     1141 taaaatcccg gttcggcgag cacctctggt tggatatcac gctccttggc gagcaccata
     1201 ttaagcacaa cctgcgcgaa gtttacgaga tctgccacta ttttcttggc gtcgatccgg
     1261 tgaaggattg ggtgccggtg cggccggcgc agcattacac gatgggcggc gtgcgcacga
     1321 attacaccgg ggagagtcct acgctcaagg gattgttctc ggccggcgaa gcggcctgct
     1381 gggacatgca cggctttaac cgattgggcg gcaactccgt ggccgagaca gtggcggcgg
     1441 ggatgatcgt cggcgagttt atcgctgatt tttgtgaacg atcggattcg ggagcgaaca
     1501 ttccgaccag cctgattcag gaaacgttcc aacatgagca agcgaaaatc gatgcattaa
     1561 tggaaggccg aggcacggaa aacgccgctg cgatcatggc cgagatgcag agtgtcatga
     1621 ccgcgaaggt cggtattttt cgcacgggcc gggtactgga agaagcggta cacgagttac
     1681 aacagctttt attgcgtagc cgcaacatcg gcctgcgcta taaggcacga ggcgcgaatc
     1741 cggaactggt gacggcctat cgcgtgcgca agatgctcaa aatggcgcta tgcgtcgcct
     1801 acggagctta cgctcgcacc gaaagtcgcg gcgctcattt tcgcgaggac ttcccgcgac
     1861 gcaacgacgc cgagtggctg aaccgcacgc tagcgacatg gaagtccgaa aacgacacgt
     1921 tgccgtctct tacctatgaa caactcgacg tgatgaagat ggagttgcca cccggctggc
     1981 gcggctacgg cgccaaggac cacatcgatc accccgacac cgccaggcgc ctcgcggaga
     2041 tcgaagcgct aaagcagtcg cgcgatgggg ttgatcgctt cgcgttccag gcgaagttga
     2101 tgccctatca acatctgttg cccgagacgt tccgcggcag aaatgaacgg atcgatgaaa
     2161 gattgtgatg aaacctgcgt cgcaccctct tctcagttgc ttcggtgcac cctatccaat
     2221 cttcccccgc taatggggaa ggctccgatc cgctcctcgc cttgatgcgg agcataaagg
     2281 agcggtgcat aaatccgaaa gccactacgc agaacaagga gcatcgcaat ggctgaaaat
     2341 cggattgaac gcgacacgat gggtgagatt gccgtccctg cggaccgcta ttggggggcg
     2401 cagacacagc gttctctcaa ttattttcag gccggtgcag ggcgcgacac gatgccgcga
     2461 gagctgatca aggccttcgg catcctgaaa aaggccgcag cgatggcgaa tgtcgagctg
     2521 aaacttttgg acaaagaaag aggcgacttg atcattcagg ttgccgatga ggtcatctca
     2581 ggcaagctcg acgatcattt ccccctggtt gtctggcaga ccggttcagg cactcagacc
     2641 aacatgaacg tcaacgaggt catcagcaat cgagccatag aaatttccgg tggggtgatg
     2701 ggaagcaaga aacccattca tcccaatgac gatgtcaaca aggcacaaag ttcaaatgac
     2761 accttccc
//
LOCUS       DASULZ010000333         2961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_447943, whole genome shotgun sequence.
ACCESSION   DASULZ010000333 DASULZ010000000
VERSION     DASULZ010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447943"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(583..765)
                     /locus_tag="VFY96_06945"
     CDS             complement(583..765)
                     /locus_tag="VFY96_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606233.1"
                     /translation="MPVPIVEPGFSIRRLPMILQTLGSIVAQNARAWSDALQSVTHDS
                     ADKRLHGSELAANWLR"
     gene            complement(835..1812)
                     /locus_tag="VFY96_06950"
     CDS             complement(835..1812)
                     /locus_tag="VFY96_06950"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX5606234.1"
                     /translation="MPEQTFLEAIRSALAEEMRRDLSVYLFGEDVALGGPFGATKGLA
                     DEFGTNRIVNTPISEGTVMGLATGAAIAGLRPVVEIMFIDFITLAMDQLVNHAAKLHY
                     MSGGQLRIPLTIRVQCGITGAMGAHHSQSLEAWLTHVPGLKVLMPATPADAKELLKTA
                     IRDDNPVVFIEHRGLYWTKAEIPEDDNVVPIGKAKVLRQGDQITILALASMVRPALIA
                     ADELAEQGISVEVIDLRSVSPLDIETIVNSGKKTSRVIVVQEAVRQSGIGAEVAALVQ
                     EQAFYHLDCPVLRVAAPFAPIPAGRSLESAFLPGKDLILEAAHRSMRSR"
     gene            complement(1864..2835)
                     /locus_tag="VFY96_06955"
     CDS             complement(1864..2835)
                     /locus_tag="VFY96_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX5606235.1"
                     /translation="MAKKILPDILPLFRRMWLIRAFEEKASLLYAERQIAGLLHLSIG
                     QEAVAVGACSLLRKDDYVFGGHRSHGHAIATGADINKLMAELAGRATGYCGGKGGSMH
                     IVSKETGFITATGVVGGTIPLALGAAFAGKQRKKGQLAVVFFGDGAGQAGAFHESLNI
                     ASLWQLPVIFVCENNGYAEFTPLSAHTKIDRLAQHAKTYAIAASTVDGNDLFAVRKAM
                     ARAIKKCRNDKGPAFVECLTHRMRGHYEGDPAKYRELSQLAEWKKQDPIARVARALRS
                     KKMITTKQTEAIEAEARAMAEKAAEFALSSPWPAAEEVDSTVYAEAR"
BASE COUNT          631 a          842 c          810 g          678 t
ORIGIN      
        1 gccaggacaa agcggatcag cggctgaatc ctgacggcac ggcgattccc ggccgaacac
       61 acgagcaagg cgcggcaaag ccagtactga ttgaaggcgc tttctttgag tagccgctaa
      121 tgaatcgcca gcattgacag gcgagctaag aagcgccgca agggccgcat tcgggtttag
      181 agattcactc tcaatcgtac gttcaacttt cctgcgggag gggagaagcc cgcgcgtgtg
      241 ggggcgcgcg caggcttcta ggggacaaat tccccgtggg actgagatcg tttacgcgag
      301 atctctcata tgagagaaag tatgttgcaa ttttcttagt tcgtcattga accagcggtc
      361 acgttgctca tgacgaagca catgagcctc cagaagcgat gccggataaa ttttagtccc
      421 gcatttttcg catttcgctg ctcggaaatc attttgatcg aacgaaatcg tttttgaatt
      481 agatgatcgt gaccgcatct tctcctccga ctccctcggt cacagcagca atgaatatgc
      541 cattggtcgc accgactcgc gagcttgatt cttttcgttt ttttagcgga gccagttagc
      601 ggcaagctcg ctgccatgca gccgcttgtc agcgctgtca tgcgtgacac tttgaagcgc
      661 gtctgaccac gcccgcgcgt tttgcgctac gatgctgcca agcgtttgca gaatcatcgg
      721 taatcgacga attgaaaaac ccggctccac gatcgggacg ggcatcgtgg cagcctcacg
      781 tggcgccgag tggttaccgc tgcagcatta ccggcgcaca gcttcgatat taaatcagcg
      841 agagcgcatt gatctgtggg ccgcctcaag aatgaggtct tttcccggaa gaaacgctga
      901 ttcgagggat cttcccgccg gtatgggcgc gaacggcgcg gcaacccgca gcacggggca
      961 atcgagatgg taaaaagctt gttcttggac gagtgccgcg acttccgctc cgatcccgct
     1021 ctgccggacg gcttcctgca ccacaattac ccggctggtt tttttcccag aatttacaat
     1081 cgtctcgatg tcaagagggg aaacactgcg caaatcgatc acttccacgg aaatcccctg
     1141 ctcggcgagt tcatccgctg caatgagcgc cggcctcacc atgcttgcga gcgcaagaat
     1201 cgtgatctgg tcaccttgcc gtaaaacttt tgctttcccg ataggcacca cattatcgtc
     1261 ttccggaatc tcggcttttg tccagtaaag accccggtgt tcgatgaaga cgacgggatt
     1321 atcgtcgcga atcgccgtct tcagcagctc cttggcatcc gccggagttg ccggcatcaa
     1381 caccttcaag ccgggcacat gcgtaagcca cgcctcgaga ctttgcgaat gatgggcgcc
     1441 catcgcaccg gtaatgccgc attgaactct gatggtcagc gggattctga gttggccgcc
     1501 cgacatgtag tggagttttg ccgcgtggtt caccagttga tccatcgcga gcgtgatgaa
     1561 atcgatgaac atgatctcca ccaccggccg aagccccgcg atggctgcgc cggtcgcgag
     1621 acccatcacg gttccttcgc tgatcggcgt attgacgatg cgattggtgc caaattcgtc
     1681 cgccaggcct ttggtggcac cgaatggtcc gccgagcgct acatcttcgc cgaagaggta
     1741 aacggacaaa tcccggcgca tttcttcggc gagtgcggaa cggatagctt cgaggaacgt
     1801 ttgttcgggc atactgactg aaaagcgcgg gcgcagcatg ctgcgcggcc tagatatcgc
     1861 tggttatcgg gcctctgcgt agaccgtgct gtctacttct tcagccgcgg gccacgggga
     1921 tgacagcgcg aattcggcgg ctttttctgc cattgctcgc gcctcggctt cgatggcttc
     1981 ggtctgtttg gtcgtaatca tttttttgct gcgtaatgcg cgtgcaaccc gcgcaatggg
     2041 atcttgcttt ttccactctg cgagctgtga cagctcgcgg tatttcgccg gatcgccttc
     2101 ataatggcca cgcatccggt gcgtgagaca ctcgacaaat gcgggacctt tgtcattccg
     2161 gcactttttt atcgctcttg ccatcgcttt acgcaccgcg aaaagatcat tgccatcgac
     2221 cgtgctcgcc gcaattgcat aagttttggc gtgttgcgcc agccgatcaa ttttcgtgtg
     2281 cgccgagagc ggtgtaaatt cggcgtagcc gttgttttca cagacgaaga tcaccggcag
     2341 ttgccacaag ctcgcgatat tgagtgactc gtggaaggcg cctgcttgac ctgcaccatc
     2401 accaaaaaaa accaccgcga gctggccctt ttttctttgt ttccctgcga acgcggcgcc
     2461 taatgccagt ggaatcgtgc cgcccacaac tcccgtcgcc gttatgaacc ccgtttcctt
     2521 ggaaacaata tgcattgacc cgccttttcc gccacagtag ccggtggcgc gacccgcgag
     2581 ttccgccatc agcttgttga tgtcggcgcc ggtcgcaatc gcatggccgt gggagcgatg
     2641 gccgccgaag acgtaatcgt ccttgcgcaa caaactgcac gcgccaactg caaccgcctc
     2701 ctgaccaata ctcagatgca acaggccggc aatctgacgc tcggcataga gcagtgacgc
     2761 cttttcctca aaggcgcgga tcaaccacat gcgacggaaa agaggaagta tgtcaggcag
     2821 aattttctta gccattaccc gtgaggatca atcaggcacg taaacgttcg attggagcga
     2881 gctcggacaa aaatagagag taaagaagga tttctcgctt gcgcgaaatg acaatcgctt
     2941 gtgatagaag acgaaaggtc c
//
LOCUS       DASULZ010000334         4691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1158115, whole genome shotgun sequence.
ACCESSION   DASULZ010000334 DASULZ010000000
VERSION     DASULZ010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4691
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158115"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="VFY96_06960"
     CDS             <1..956
                     /locus_tag="VFY96_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX5606236.1"
                     /translation="KIEVLPAGAVVPAFGVLDAVSKGTLDGAHGVLSYHYGKSNALAL
                     WGSSPAYGMDANTILAWHRYGGGKELLSKIYNSLGANVVSFPYGPMPTQPLGWYKKPV
                     TKPEDFKGMKFRTVGISIDLFQGMGASVNALPGAEIVPAMDRGLLEAAEFNNASSDRA
                     LGFQDVSKVCMLQSYHQNGEQFEIIFNKTKFDALPEKMRAIIAYAVDAASADMSWKAI
                     DRYSKDYVELQTKEKVRFYRTPDSVLQKQLEIFDQVEAKKSAEVPMFKEVAESQRAFA
                     ARAVKWDLDTNVGRRMAYNHYFSKRGAKPGTPAAKSEAKKS"
     gene            1068..1775
                     /locus_tag="VFY96_06965"
     CDS             1068..1775
                     /locus_tag="VFY96_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX5606237.1"
                     /translation="MNAQRFLRGIDQISYWSGKAFAWLIVALTFVVSIEVFKRYILNA
                     PTAWIFDFTNMLYGTLFMMCGAYTLALSAHVRADFVYIYLKPRGQAALDLTLYFLFFI
                     PGIIGLIYAGYGYAADSWRIGEHSNITAEGPPIYYFKTVIPIAGALVLLQGFAEIIRC
                     IECLRSGAWPARLADVEEIDVIDTQLSRSEYVDEESRRTAMQTAHAVDEAARHRSVME
                     ESVVHERAIREDEQKKS"
     gene            1772..3808
                     /locus_tag="VFY96_06970"
     CDS             1772..3808
                     /locus_tag="VFY96_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX5606238.1"
                     /translation="MSDPALGLTMLCLIVVAIMMGFPTAFTLMGLGMIFGYLAFWEPA
                     QHWWQNRIFDLIVQRTYGVMTNDTLLSVPLFVFMGYIMERAALVDRMFHAVQLAFRRV
                     PASLAVTTMLVCAFWGIASGIVGAVVILMGVIAMRPMLNAGYDVRLASGAITAGGTLG
                     ILIPPSVMLIVYAAVAGQSIVKLYAAAMLPGFFLTFLYLVYILGWAMINPKIAPKLSE
                     DQYRVTVPDWLKRLEHGPSRSIFFGIVAAIFRPDLTRGSASSDGSRISYGRIMQNFIA
                     LMVPLALTAGTLAATWWYVVIYNAPSAQVESAPAAVPPTPQTAAPQAGSPAEEKPQEL
                     GEAESEIKGAEPQRGADAPPEEMTIAGEPSAQSAAGKVPAHFYDWFWGLAAASALLLA
                     YYYWRMDGEQFEILKELSASVVPLGVLTVVVLAVILFGICTATESAAVGALGALYLAV
                     IAKFPHQAYTWSVPGVIGGVALGWAHADFVELIVSGSLGATFAGTVIPILQNLKSSRE
                     LRQNIKEATFLTAKTTAMVCWLFIGSALFSAVFALHGGQSLIERWVLSMNLSPLGFQF
                     TAQLIIFLLGWPLEWTEIIVIFCPIFIPLLSHFQIDPILFGTMVAVNLQAAFLSPPVA
                     MSAFYLKGVSPPHVTLNQIFAGMMPYMLIVCLCLAFMYIWPGMTLWLPEFLYGN"
     gene            4082..>4691
                     /locus_tag="VFY96_06975"
     CDS             4082..>4691
                     /locus_tag="VFY96_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX5606239.1"
                     /translation="MARIYDVDPGVQAWAFLDPTYALAQARAADERRLNGPPTGALHG
                     VPVAIKDIFDTADFPTEYGSPLFAGHTPSRDATVVARLRAAGAVILGKTVTTEFAYFS
                     PGKTRNPQNTEHTPGGSSSGSAAAVGAEMVPLSIGSQTNGSTIRPAAYCGVVGFKPTH
                     GLISRYRALALSRTFDHVGLFARSIADIALMTEQLVGHDENDP"
BASE COUNT          910 a         1331 c         1357 g         1093 t
ORIGIN      
        1 ttaagataga agtgctgccg gcgggtgcag tggtgccagc gttcggtgtg ctcgatgccg
       61 tctcgaaggg cacgctcgac ggcgcgcacg gcgtcctctc gtaccattac ggaaagagca
      121 atgcgctcgc gctgtggggc tcgagtcctg cctacgggat ggatgcgaat acgattctcg
      181 cctggcacag atatggtgga ggaaaggaac tgctcagcaa aatctacaac tcgctcggtg
      241 ccaatgtcgt ctcgtttcct tatggaccga tgccgactca gcccctcggc tggtacaaaa
      301 agccggtgac caagccggag gatttcaaag gtatgaaatt tcgcaccgtc ggtatctcca
      361 tcgatttgtt tcagggcatg ggcgcatcgg taaatgcgct gccgggagcc gaaatcgttc
      421 cggcaatgga ccgcggcttg ctcgaagctg cggagttcaa caacgcatct tccgatcgcg
      481 ccctgggttt ccaggacgtc tccaaggtct gcatgctgca aagctaccac cagaacggcg
      541 agcagttcga gattattttc aacaaaacca agtttgacgc gctcccggaa aaaatgagag
      601 cgatcatagc ctatgctgtg gatgccgctt ccgcggatat gtcctggaaa gcgatcgacc
      661 gctattcgaa ggactatgtg gagcttcaga ccaaggagaa ggtgaggttt tacagaactc
      721 cggattcagt gttgcaaaag cagctcgaga ttttcgatca ggtggaggct aaaaaatccg
      781 ccgaagtccc gatgttcaaa gaagtcgctg aatctcagcg cgcgttcgct gcacgggccg
      841 tgaaatggga cctcgatacc aatgttggcc ggcgcatggc ctataatcac tacttttcca
      901 agagaggcgc caagcctggc actccggcgg cgaaatcgga agcgaaaaaa agttaactgt
      961 ctcgtaactc gcaccaacgt gcatctgctg gctgtaccgg ttgtagccgt tgaattccgc
     1021 ctcacgcaag caattcggct tactctcaca aaaggcgatg tatagccatg aacgcccagc
     1081 gcttccttcg cggaattgat cagatcagtt actggtcagg caaggccttc gcatggctca
     1141 ttgtcgcgct gacgttcgtc gtttctatcg aagtcttcaa acgctacatt ctgaatgctc
     1201 cgacagcttg gatcttcgac ttcaccaaca tgctgtacgg cacgctgttc atgatgtgcg
     1261 gtgcttatac gctggcgctc agcgcgcacg tgcgcgctga ttttgtttac atctacctca
     1321 agccgcgcgg ccaggcggcg ctagatttga cgctctactt cttatttttc attccgggaa
     1381 ttatcggcct catctatgcc ggttacgggt acgccgccga ttcgtggcgt atcggcgagc
     1441 actccaatat taccgcggag ggtccgccga tctactattt caagaccgtg atcccgattg
     1501 ccggcgcttt ggtgctgctt cagggtttcg ccgagatcat ccgctgcatc gaatgcctgc
     1561 gcagcggcgc ctggccggcg cggctggcgg acgtggaaga aatcgacgtg atcgacaccc
     1621 agctctcccg tagcgaatac gtcgacgagg aatcacgccg cacagcgatg cagaccgcgc
     1681 atgcggtcga cgaggcggcg cgccaccgca gcgtcatgga agagagcgtg gtgcacgagc
     1741 gcgcgatcag agaggacgag cagaaaaaat catgagcgat cccgcgctcg gtctgacgat
     1801 gctctgtctc attgtggtcg cgatcatgat ggggtttccc accgcattca cgctgatggg
     1861 cctcggtatg atcttcggat acctcgcatt ctgggagcca gcgcagcact ggtggcagaa
     1921 cagaatcttc gacctgatcg tgcagcggac ctacggcgtc atgaccaatg acacactgct
     1981 gtcagtgccg ctgttcgtat tcatgggcta tatcatggaa cgagcggcgt tggtcgaccg
     2041 gatgtttcac gcggtgcaac tcgcctttcg gcgcgttccg gcgtcactcg cggtgaccac
     2101 gatgctcgta tgtgcatttt ggggcatcgc ctccggcatc gtcggcgcag tggtgattct
     2161 gatgggcgtg attgccatgc gccctatgct caacgccggt tacgacgtgc ggcttgcatc
     2221 cggggcaatt accgcaggcg gcacgctcgg cattctcatt ccgccttccg tgatgctgat
     2281 cgtctatgca gcggtggccg ggcagtcaat cgtaaaacta tacgctgcgg cgatgctgcc
     2341 gggttttttt ctgacgttct tgtatctcgt ctacattctc ggctgggcaa tgatcaatcc
     2401 aaaaattgct cccaagctgt ccgaggatca atatcgagtt accgtacctg attggctaaa
     2461 gcggctggag cacgggcctt cacgcagcat ttttttcggc attgttgcgg cgatatttcg
     2521 tcccgatctg acccggggct ccgcgtcttc cgatggaagt cgaataagct acggtcgtat
     2581 catgcaaaat tttatcgccc tgatggttcc gttggcgctt acggccggca cattagccgc
     2641 gacctggtgg tatgtggtga tctataacgc gccttcggcg caggttgaaa gcgctccggc
     2701 tgccgtgccg ccgacgccgc agacggcagc cccacaggcg ggcagtcccg cagaagagaa
     2761 gccgcaggaa ttgggtgaag ccgagtctga gatcaagggc gcagagccgc agcgcggcgc
     2821 cgatgcgccg ccggaagaga tgactatagc cggagagcct tctgcacaat ctgccgccgg
     2881 caaagtgccg gcgcattttt acgactggtt ttggggcttg gcggcggcct cggctctgct
     2941 gctcgcctat tactactggc gcatggacgg cgaacagttt gagatcctca aagagttatc
     3001 ggcatcagtc gtgccactgg gtgtgctcac ggtggtcgtg ctggcggtaa tcctattcgg
     3061 catatgcacc gccacggaat cagccgccgt gggggcgttg ggcgcgcttt atttagcagt
     3121 gatcgccaag tttccgcacc aagcgtacac gtggagcgtt cccggcgtga ttggcggtgt
     3181 ggcgcttggc tgggcacacg ccgattttgt cgagctgatc gtttcgggct ctctcggcgc
     3241 gacgttcgcc ggcacggtga tacctatcct gcaaaacctg aagtcgtcgc gcgagctgcg
     3301 ccagaacatc aaagaagcaa cttttctcac ggcgaaaacg accgcgatgg tctgctggct
     3361 tttcatcggc tcggcgcttt tctctgcggt gttcgcgctt catggcggcc aatccctgat
     3421 cgagcgctgg gtcctgagca tgaatctctc gccgttggga tttcagttta cggcccaact
     3481 gatcatcttt ttgctcggtt ggccgctcga atggacagag attatcgtta tattctgtcc
     3541 gatctttatt cctctgctca gccacttcca aatcgatccg attctcttcg gaaccatggt
     3601 cgccgtgaat ctccaagcgg cttttctttc accgcctgta gccatgtcgg ctttttactt
     3661 gaaaggcgtc tcgccaccgc acgtaaccct caatcagata ttcgctggca tgatgccgta
     3721 catgctcatc gtctgcctgt gcctggcgtt catgtacatt tggcctggca tgacactgtg
     3781 gctgccggaa tttttgtacg gcaattagtg ccacgacgat aagcgtcgca tcgctgcgtt
     3841 ctcggtcggc cggcagcctc aacgtactga cgatgtacgt ttccggtgcc cttctctctc
     3901 gggcctgacg ctgctcgctt ctcatcgtgg cacccgatgg gaatgtttgc ggtctctgaa
     3961 gatcctggct caaagacgtt cggtcatgga attgacgaat cttcatttgc tctcagccgc
     4021 tgaagcggcg aatttgattc acactgggat tatcagctcg gtacagttgg ttgagtcatg
     4081 cttggcacgg atttacgacg ttgatcctgg agtgcaggcc tgggccttcc tggatccaac
     4141 ttacgccttg gcgcaggcgc gcgcggcgga tgagcgacgt ttgaacggcc cgcccactgg
     4201 cgcgcttcac ggtgtgccgg tagcgatcaa agacatcttc gatactgccg actttccgac
     4261 agaatatggc tcaccacttt ttgccgggca tacgccatcg cgtgacgcca ccgtggtcgc
     4321 acggttgcgc gccgctgggg cggtgatttt ggggaagacg gtcaccacgg agttcgccta
     4381 tttttcgccg ggtaagacgc gtaacccaca aaacaccgag catacccccg gcgggtcatc
     4441 gagcggctcc gccgctgccg taggggccga aatggtaccg ctctcgatcg gtagtcaaac
     4501 caacggctcg acaattcgcc ccgcggccta ctgcggcgtg gttgggttca aaccgacgca
     4561 cggcttaatt tcgcgctatc gtgcgctggc gctgtcgcgc accttcgatc acgttggatt
     4621 atttgcgcgg tcgatcgccg atattgcgct aatgaccgaa cagttggtgg gccatgatga
     4681 aaatgatccc g
//
LOCUS       DASULZ010000335         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1158336, whole genome shotgun sequence.
ACCESSION   DASULZ010000335 DASULZ010000000
VERSION     DASULZ010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158336"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VFY96_06980"
     CDS             complement(<1..425)
                     /locus_tag="VFY96_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX5606240.1"
                     /translation="MLLSDSFAGSPPIQNRKSKIQNSSTDLTRAKKVALSCRILAKLG
                     LFKETTGHVSVRSATGAAMLIRGRGKEETGLLFTRPSDVVLADFDGQRLEKRNVLKAP
                     NESVIHGELYKARPDVAAIVHAHPASIVLTGMAGIELRP"
     gene            739..1605
                     /locus_tag="VFY96_06985"
     CDS             739..1605
                     /locus_tag="VFY96_06985"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX5606241.1"
                     /translation="MTEPDDAIGTEQLQRIEGVFTEEVLPESLVQQMPQLKWLHVTRG
                     GVNAYLTPSVKARPIQVTGSKGIHGTVFSEFALACILMVAKKLPDCIAVQREKRWQKL
                     APMELERKTLGIVGLGTAGSALARKAKAFGMRVLATKRTSAFKPEFVDQLGTPEFLPQ
                     LLAESDFVVLLLASVPSTFNIIGENELRRMKPSAYLINLTGGRAIEENLLIRALKERW
                     IAGAVLDAFARQPLPQESELWSLPNVIITPRIAGITSEKWAALLPIFTENLQRFITGK
                     PLNNLVNKELGY"
     gene            1915..>2267
                     /locus_tag="VFY96_06990"
     CDS             1915..>2267
                     /locus_tag="VFY96_06990"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606242.1"
                     /translation="MASILYVATDEGVVTLKSEDGHNWQQLHHGLKDWAIPEVVVSPN
                     SPNKVFAGTRGDGVWLSEDFGATWKKPCYGKRGPGKVRCLTFDPHDSKTLYTGTEPID
                     VFVTRDEAKSWERLD"
BASE COUNT          542 a          634 c          595 g          496 t
ORIGIN      
        1 atgggccgca attcgatccc cgccatgccg gtcaatacga tcgaagccgg atgcgcatga
       61 acaatcgccg ccacatcagg tcgcgccttg tagagctcgc cgtgaatgac cgattcgttc
      121 ggtgccttga gcacatttct cttttcgagc ctctgtccat cgaaatccgc aagtactaca
      181 tcgctcggcc tggtaaagag taagccggtt tcctctttgc ctcgaccgcg gatcagcatc
      241 gccgctccgg tcgcgctgcg cacgctcacg tggccagtgg tttccttgaa taatccaagt
      301 ttagcgagaa tgcggcagga caatgcaact ttttttgccc gcgtcaaatc agtcgaagaa
      361 ttttggattt tggatttccg attttggatt ggaggactcc cagcgaagct atcgcttagt
      421 aacacgcgac cagcggtccg tcaacgaatc accctgaact gtgattgatc ccggttcatc
      481 ataaccttgg aaacgaagaa agattcctcg cttcactcgg aaatgaacag ctaaaaaaat
      541 gactatcatc tcgaagccct tcgacatgct cagagtaaac tccgcgagaa aatgcttccc
      601 aaactcagaa tgtctgcgtt gacacctcgc ggcggctgct atacgtgaca ttccatgtcc
      661 acgacaattt ggttgcgcgt aagcgttccg gaaaccgatt tgaatgcgct gcgcagcgat
      721 ttttcgagct gtgaactcat gacggaacca gatgatgcca tcggcactga gcagcttcaa
      781 agaatcgaag gcgttttcac ggaagaagtt ttgccggagt cgctcgtgca gcaaatgccg
      841 caattaaaat ggcttcatgt gacacgcggc ggcgtcaatg cttatcttac tccctcagtc
      901 aaagcgcgtc cgatacaagt caccggctcg aagggaatcc acggcacggt gttttcggaa
      961 tttgcgctcg cgtgcattct aatggtcgcg aaaaaactgc ctgactgcat cgccgtacaa
     1021 cgggaaaagc gctggcagaa actcgcgccg atggagctcg aacgaaagac acttggaatt
     1081 gtcggactcg gaacggcggg ctcagcgctt gcgcgcaaag ccaaagcctt cggcatgcgc
     1141 gtcctcgcca ccaagcgaac atcagcgttt aagccggagt tcgtcgatca gttgggcacc
     1201 ccggaatttt tgccgcaact gcttgcggag tcggatttcg ttgtcctgct tctcgcatcc
     1261 gtgccctcga ccttcaacat tatcggcgag aacgaattgc gcaggatgaa accgagcgct
     1321 tatctgatca acctcacagg cggacgcgcc atcgaagaaa atttgcttat acgggctctc
     1381 aaagagcgct ggatcgccgg agccgtgctc gacgctttcg cgcgccaacc gctgccgcag
     1441 gaatccgaac tgtggagcct acccaatgtc atcattacgc cgcgcatcgc cggcatcacc
     1501 agcgaaaaat gggctgcgtt gctgccgatc ttcacagaaa atctgcagcg cttcattacc
     1561 ggcaagccgc ttaataattt ggtcaacaag gaactgggct actaatttca gctttcaatg
     1621 agcggcaatc agaagctgca aacgactgga tctcggctgg agcttatcct gggcacagcg
     1681 aagggccggt ataacagccc ggttgtgagc gaacgtagcc caagaaaccc gtggaattcg
     1741 ggactcctgg attgcgcttt gctttatgcc gggctacaga tctccaggcg tcccctgacg
     1801 atttgaatgg cttgaacgtt ttgaacgact ggaacgggac ctgtcgcgct cgccttgtcg
     1861 gccttatagt tctgtagtag atattcaacc atccaaaacg ggaggaaaag aatcatggca
     1921 tcgatcctct atgtcgctac tgatgaaggc gtagttactc tcaaaagcga agacggacac
     1981 aattggcagc agctccatca cgggctcaag gactgggcga ttccggaagt cgtcgtttcg
     2041 ccgaattcgc cgaacaaagt ttttgccgga acccgtggcg acggcgtctg gctaagcgaa
     2101 gatttcggcg cgacctggaa aaagccctgc tacggcaagc gcggtcccgg taaagtgcgc
     2161 tgcctgacct tcgacccgca cgatagcaaa accctctata ccggcaccga gccgatcgat
     2221 gttttcgtca cccgcgatga agccaaaagc tgggagcggc tcgacag
//
LOCUS       DASULZ010000336         2080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1461963, whole genome shotgun sequence.
ACCESSION   DASULZ010000336 DASULZ010000000
VERSION     DASULZ010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461963"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..297)
                     /locus_tag="VFY96_06995"
     CDS             complement(73..297)
                     /locus_tag="VFY96_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017866784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606243.1"
                     /translation="MRAMAILLLLGSVAITGCEFAAGAATGALATGAGYEYNSKRQMD
                     RLEEDYRNERISRSEYERRKSQIERGSIVY"
     gene            complement(1011..2009)
                     /locus_tag="VFY96_07000"
     CDS             complement(1011..2009)
                     /locus_tag="VFY96_07000"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606244.1"
                     /translation="MRGLIFCLISAALALASTANSSYAQTRLKKIRVGVPSVGMGNII
                     IFVTKEAKLFEKYGLDAEVITVMGSGIGSKALISGNLDIIPIATPTVIAADLAGADMA
                     ILAHTMPAVVHALMVRPDIKRPEDLKGKKIGVSSLGSLTDFLVRSIAKKKGLNPDRDI
                     TLITTGGSDTERVMALKAGAVDATAVSHPGYGMAQRMGYSMLWDSAKELDYPWMEITT
                     RRAAVKNDSDLIMRYMKAHLEGIALFKRDRNFGIKVIKKTLKMTDEELVNESYDIFSK
                     MFIPAPYPNNPGMKISFEYVAATRPDVWNHKPEEFTDSSFVAELDKSGFIKSLYGK"
BASE COUNT          482 a          560 c          531 g          507 t
ORIGIN      
        1 taagcgagtg cgagtgctcc tgcgcctcga agagcgcctg gagcactcgc ctgttatatc
       61 agtacgcgca atttagtaaa cgattgagcc gcgttcgatc tgactcttcc gccgttcgta
      121 ttcgctccgg ctgattcgct cgtttcgata atcctcctcc agccgatcca tttgccgttt
      181 cgagttatac tcatatcccg cgccggtcgc caaagcgccg gtggcagctc ccgcagcaaa
      241 ttcgcagccc gtaatcgcta cacttccgag caatagtaaa atcgccatag ctctcataat
      301 caatccctcc tctcaaatgc ttgtgaagca agggagcaac tcccggacca acataaaacc
      361 tgcaaaactt tgatgactta cctataaacg agattccgct aaaccgcttt ggtgggaatc
      421 ggccggtagg aattttctaa cagcggacgt tttttgaaac ggccgatcgt ggacactgtc
      481 tctgattgga aaaaatgacc gagatgtcat ttccaatgcc cgtttcgaca gctgaccaga
      541 tcgttaaggc gctccttatg aaaagccctt gctcgtttgc atgcttctga ttgatgggta
      601 aacccggatc attccggtgc ggcttagaag ggcggccgct gggagcgctc gggctaaccc
      661 agaacttgat ttcaagacga tcacgatcgc attgacgtga aacggagtgc cggacccttt
      721 tggcaattaa gaatagaagt caggaacgcc cccattgaaa ttcgcgaaat ggtcgtcagg
      781 ttcgctgacg ggaaaacttt caagccaaaa atacaggcgc gctttcgcga gggaagtggc
      841 agccatgtca acgatttgcc gggaaagcaa agatctatcg acggagtaga gtttgttatt
      901 acagcatcaa ccgtaaacag ggcaggggcc gagtcttgtt gtatgctcgc taatccgtga
      961 agttgtattc acgaccctgc cgcttcatca ggaagcgcga accagggaga ttattttccg
     1021 tagagggact ttataaagcc gctcttgtcc aattccgcga cgaagctgga gtcggtgaac
     1081 tcttccggct tgtgattcca gacgtcaggc cgggtggcgg cgacatattc gaagctgatc
     1141 ttcatcccgg gattattggg atatggcgcc gggatgaaca tcttcgaaaa aatgtcgtag
     1201 gactcattga ccagctcttc gtcagtcatt ttcaaagttt ttttgatcac cttaatgcca
     1261 aaattgcgat ctcgtttgaa cagcgcgatg ccttcgagat gtgctttcat gtagcgcatg
     1321 atcaaatccg aatcgttttt gaccgccgct cgccgcgtgg tgatttccat ccatggataa
     1381 tcgagctcct tggcggaatc ccagagcata ctgtatccca tgcgctgcgc catgccgtac
     1441 cctggatgag aaaccgcggt agcatcgaca gcgcccgctt tcagcgccat cacccgctca
     1501 gtatcgctgc cgccggtggt aataagagtg atatcgcggt cggggttgag tcctttcttc
     1561 ttggcgatcg agcgaaccaa aaagtccgtt aacgacccca gacttgacac tccgatcttt
     1621 tttcctttca aatcctcggg ccgcttgatg tcaggtcgca ccattaacgc atgcaccacg
     1681 gccggcatcg tgtgcgccaa tatcgccata tcggcaccgg ccagatcggc cgcgatgaca
     1741 gtgggcgtgg cgatgggaat aatgtccagg ttaccactga tcaaagcttt cgagcctatg
     1801 cctgagccca tgacggtgat gacctcggca tcgagcccgt atttttcaaa gagcttcgct
     1861 tctttggtga caaagataat gatgttgccc atgccgacgg acggcacgcc gacgcgaatc
     1921 tttttgagcc tggtttgcgc gtagctgctg ttcgccgttg aggctagcgc cagcgctgcg
     1981 ctgatcaggc aaaatatcag tccacgcata aagatctcct ggtcgcgaga gctcatacca
     2041 tctgcatata gacgtcgacc aaagcagggc gcttctgttc
//
LOCUS       DASULZ010000337         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1259764, whole genome shotgun sequence.
ACCESSION   DASULZ010000337 DASULZ010000000
VERSION     DASULZ010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259764"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="VFY96_07005"
     CDS             <1..1039
                     /locus_tag="VFY96_07005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5606245.1"
                     /translation="SEQQTYADVLEQIALADELGYDFFSTTESYGKDLFSCSPFPLGL
                     YVAAAHGTQRIRFLTGIVSAPAHHPAVLASEIAAADLLTGGRIMLGIGRGHPWVLNRL
                     GLNPAESTARFEESLRMLVEIFKSDYIESLEGQFWQIRDFKLSPPPLQQPHPPIYTAA
                     ATTPESAIFAGKIGQGLVQPGYLGIPFDLVRGLTETYRRNISASRSSDVILGIHLHVA
                     RDRERAIENGALALSSQADVFLRSSQSRPNLSGVKEAYATKSVSRMAFEKLSTPTKAT
                     QALAKEGTSIMAVWGTPNDCIEKIKFYVDALSPEQLMLNIASGSLPQEKVLEAMRLFA
                     EQVMPALRHFV"
     gene            complement(1369..>2083)
                     /locus_tag="VFY96_07010"
     CDS             complement(1369..>2083)
                     /locus_tag="VFY96_07010"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5606246.1"
                     /translation="HTGWNYSGAPGTKAIGIGDEAAGVSMVCGILAALHARERTGEGQ
                     RIVVSMQEALLGFMISEFHEHFIGIEIGNKPVPVADGYFTLRIPELSDRGWIELAKFM
                     ERDDLIEDARFATASARKQNRSELYDLIRAWIRGKNRQELWDGLRKIDYFGAPVLSMG
                     EVLEDPHIKARNAFIERNHPKAGPTTLLAPWIHMSKTAPSIRADAPLLGQHTDEVLGG
                     VLGLSSSELAALRAESVIK"
BASE COUNT          418 a          597 c          610 g          458 t
ORIGIN      
        1 gtcggagcag cagacttatg ctgacgtcct ggagcagatc gcgcttgccg acgagctggg
       61 atacgatttt ttctccacca cagaaagcta cggcaaggat ttattttcct gctcgccctt
      121 tccgcttggg ctttatgtgg cggcggcgca cgggacccaa cgcattcggt ttctcaccgg
      181 tattgtcagc gcgccggcgc atcatcccgc ggtgctcgcg agcgaaattg ccgccgctga
      241 cttgctcacc ggagggcgta tcatgctcgg gatcggacgc ggccatccat gggtgctcaa
      301 tcggcttggc ctcaacccgg ccgagagcac ggcgcgcttt gaagaaagcc tgcgcatgct
      361 ggttgagata ttcaagtccg attacatcga gagcctggag gggcaattct ggcaaatcag
      421 agattttaaa ttgagtccgc cgccgctgca gcagccgcat ccgccgattt acacggccgc
      481 agcgacaacg cccgagagcg cgatttttgc ggggaagatc ggccaggggt tggtgcagcc
      541 gggttacctc ggaattcctt tcgatttggt gcgagggttg acggagactt accgcagaaa
      601 tatttcagcc agccgatcga gcgatgtgat tttagggatc catctacacg tggcacgcga
      661 tcgggaacgg gcgattgaaa acggtgccct cgcgctttcg tctcaagccg atgttttcct
      721 acgctcgagt cagagtcgac ccaatttgag cggcgtgaag gaggcctacg cgaccaaaag
      781 cgtgtcccga atggcgttcg agaaattatc gacgccgacg aaagcgacgc aagcgttggc
      841 gaaagaaggg acaagcatca tggcggtgtg gggcacaccg aatgattgta tcgaaaagat
      901 caagttttat gtcgatgctc tttcacctga gcagctcatg ctcaatatcg catcgggaag
      961 tctgccgcag gaaaaagtgt tagaagcaat gcgcttgttc gcggagcaag tgatgcccgc
     1021 gttacgccac tttgtttgac tgttgactga ttgcccgcct tcacggcagc tgcataacgc
     1081 ccatcgcccc tcgctacgcg accgcggacg gccgcgactc ggggcctcgg cagtttgatg
     1141 tgcttggttc acggtgtggg cactccgctg atcaaccgat gaaatcttcg ttccaaggag
     1201 ccgaaccgat tggacttaat ccgatttccc gagtagcgcg aagggtatcc tttgatacgg
     1261 cagcgtcagc cgctgcctac tcaggatgtc ggataaaccg ggtcgttagc cgcggagttt
     1321 gaatctgttt tgatctaagc aagcgcgacg tcaacaccgt gtggtgggct atttaattac
     1381 actttccgct cgcagcgctg cgagctcgct ggaactcaat cctaaaactc cgccgagaac
     1441 ttcatcagtg tgctgcccca gcagcggcgc gtcggcgcgg atgctcggcg ctgttttcga
     1501 catgtgaatc cagggagcga gcaaggtcgt agggccggcc ttcgggtgat tgcgctcgat
     1561 gaaggcgttg cgtgccttga tgtgcggatc ttccaacacc tcgcccatcg atagcactgg
     1621 cgcgccaaag tagtcgatct tgcgcaaacc gtcccaaagc tcttggcggt ttttgccgcg
     1681 tatccaggcg cggatcaggt cgtagagctc tgagcgattc tgtttacgcg ccgacgccgt
     1741 ggcgaaacgt gcgtcttcga tcagatcgtc gcgttccatg aacttcgcga gctcaatcca
     1801 accgcggtcg ctcagctccg gtatccgcaa agtaaaatag ccgtccgcga ccggaaccgg
     1861 tttgttgccg atttcgatgc cgatgaagtg ctcgtgaaat tcgctgatca tgaagccgag
     1921 caacgcttcc tgcatggaca cgacgatcct ttgcccttcg cccgtacgtt cgcgcgcatg
     1981 gagcgccgcc agaatgccgc acaccatgct cacccccgcg gcctcgtcac cgatgccgat
     2041 cgctttggtg cccggcgcgc cgctatagtt ccagccggta tgt
//
LOCUS       DASULZ010000338         4714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_143967, whole genome shotgun sequence.
ACCESSION   DASULZ010000338 DASULZ010000000
VERSION     DASULZ010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143967"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..450)
                     /locus_tag="VFY96_07015"
     CDS             complement(25..450)
                     /locus_tag="VFY96_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5606247.1"
                     /translation="MAQAHVLRIDSLPVIDRGNGIQTTPLVTKEIGLQHLTTGITRFP
                     VGARVPMHSHNCDEQVTILEGEAEAEIGAERHRVRPYDTTLVPANLPHRFVNVGTAPL
                     AILWIYTAAEVTRTFTETGETVAHLSERDFAGETNVAKK"
     gene            complement(599..1609)
                     /locus_tag="VFY96_07020"
     CDS             complement(599..1609)
                     /locus_tag="VFY96_07020"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606248.1"
                     /translation="MSHRQRFSVACSLLCFVCLASSAETGAQSAALTAFYTAPVASMA
                     PMWIAKEVGFFKKHGLDVKLVFIASGPVGTASVLGSETDVGIIGGFAPLRAIVGGAKG
                     LVIIGQSKNRITSKIVGKKEIASVQDLKGKRLGIDRIGSNPDMFAQAALSRFQIDTFK
                     DLQYVQLGDVGKVVAALKAGAIDAGIAGAPHDLFAQRLGFKVILDIALLKIPFAATVL
                     MSGRDTVARKQPELTRFMRAYAEAMHYFLTNPEGTANVVTKYTQVTERDVLAYSIDSE
                     AAAMEKTLAIDPKGLELILEFIGKTVPQAASAKPEDFYDSRFVNDLRTSGLLKKLWGE
                     KL"
     gene            complement(1728..2735)
                     /locus_tag="VFY96_07025"
     CDS             complement(1728..2735)
                     /locus_tag="VFY96_07025"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606249.1"
                     /translation="MIFQFKRMTTLGIAFMASMSLSTIVCAQSSTLTAFYTAHVVSMA
                     PMWIAKETGLFKKYGAEVRLVYIGSGPVGTTSILSGETDVGIIGGFAPIRAILGGAKN
                     LVIIGQSKHAMVGAIVGRKDITGVQELKGKRLGIDRIGSNPDMYTQAALAQFQIDPLK
                     DLSYVQMGSTSQGIPALKAGSIDAFTTTPPYDLFAQRLGFKVILDITALKIPFAATVL
                     LSTRSTVSRKQAEIAKFSRAYAEAIHTFLTRPEIAAKIVQKYTKVEDQEVLHHSMAAE
                     GDAMEPTLQVDPKGIELILGLIRKSVPQAASAKADDFFDPRFFTELKTSGFIDRLWGK
                     S"
     gene            2958..3932
                     /locus_tag="VFY96_07030"
     CDS             2958..3932
                     /locus_tag="VFY96_07030"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5606250.1"
                     /translation="MGESRRKNWRIDVHHHVVPPRYADDSMPIKLPDAEEQLGSMDRW
                     NIRAAITSLTPRVVLKNLDRLRSVARECNEFQAQCVRDHAARLGAFALLPLPDVDGAL
                     QEIAYALDVLRLDGIGLFSSITHRYLGDPQFDPVFDELDRRGAVVFIHPTHCEAPEDT
                     RLQAPPFVVEYVFDTTRAMVNLIYTGTLQRCPNIRFIVAHGGGTLPFLAQRVAMMEGH
                     RGGKNVTGVLPALQSLYYEIASTTSPYALRSLQELVRPTNILWGSDLPFVYGDRLRDE
                     VEHWEHYTGFDTAGRAAIEAGNAQRLFSRFAQTVSGERDSMVFLKEEL"
     gene            3929..>4714
                     /locus_tag="VFY96_07035"
     CDS             3929..>4714
                     /locus_tag="VFY96_07035"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606251.1"
                     /translation="MRLLKWRQWVVLSLMMVLPLVSIARFTSAADSGRFLVSYGGTAG
                     YQLPLWVNKEFGFSKKYGVDLEIILIQAGSPNIQALVGGSLQLTQTAASSAVIGASQG
                     APVVIVATLENKLPMLLISRPEIKDPQQLRGKVIGINRFGGSNDAAVLMAMKAWKIDP
                     KEISMLQTGGTSARMAALISKKVDATVQSYPEIYQARKLGMNVLADIGDFGFYTNTSN
                     IVTRSYVQQNRAAIKGFIKGQIEGIHYVKTNKEGSLKVLRKHLQ"
BASE COUNT         1034 a         1270 c         1323 g         1087 t
ORIGIN      
        1 tggggaaaat agtgagcggc caggttattt tttcgcaacg ttcgtctcac ccgcgaaatc
       61 ccgttcggac aagtgagcta cagtctcgcc cgtttcagta aaggttcggg tgacctccgc
      121 tgcggtatag atccatagaa tcgcgagcgg cgcggtaccc acgttcacga aacggtgcgg
      181 taagttcgct ggaactagag tcgtgtcata gggacgaacc cggtggcgct ccgcgccgat
      241 ctcggcttcg gcctcgcctt ccagaatagt tacttgctca tcgcagttgt gcgaatgcat
      301 gggaactctc gcgccgacgg gaaaccgagt aattccggtg gtcagatgct gcaagccgat
      361 ttctttcgtt accagcggag tggtttggat gccattgccg cgatcgataa ccggaagcga
      421 atcaattctc aaaacatgag cttgcgccat gagagtcgct cgtttttaca ggttggatgc
      481 actaccggcc gcggagtaaa agcgttcaaa gcgttctaat cgtttcaaat tttcactccg
      541 ctccttcgtc ctcccccggc gcggggagga aagagatggg ggaagaattt cgcacgctct
      601 acaacttctc accccacagt tttttgagca atccgctcgt acgtaaatcg ttaacaaaac
      661 gcgaatcgta aaaatcctcc ggcttggctg aagcggcctg aggcacagtc ttgccgatga
      721 attcaaggat gagctccagt ccctttgggt cgatcgcgag agttttttcc attgctgccg
      781 cctccgagtc gatggaataa gcaagtacgt cacgctcagt gacttgggtg tatttcgtca
      841 cgacgtttgc agttccttcc gggttggtca ggaagtagtg catcgcttcg gcgtacgcac
      901 gcataaatct ggtaagctca ggctgtttac gcgccacagt gtcccgtccc gacatgagca
      961 ccgtggcggc aaaaggaatt ttcagcaagg cgatatcgag aatgactttg aagcccaagc
     1021 gctgcgcaaa aagatcgtgc ggcgcacccg cgatgcccgc gtcaatcgcg ccggccttca
     1081 gagccgcgac gaccttgccg acgtcaccga gctggacgta ctgcaaatct ttaaacgtat
     1141 cgatctgaaa tcgggaaagc gcggcctgag caaacatgtc tggattcgaa cctattcggt
     1201 cgatgcctaa ccgcttacct ttaaggtctt ggacgctggc aatttctttc tttcccacga
     1261 tcttactggt aatgcggttt ttactttgtc cgatgatgac taagcctttg gcgccgccga
     1321 cgatcgcccg taaaggcgca aagccgccga tgatccccac atcggtctcg ctgccgagca
     1381 cggaagctgt gccaaccgga ccggaagcaa taaaaactaa tttgacgtca agtccatgct
     1441 ttttaaaaaa tcctacttct ttagcgatcc acatcggcgc catagaagcg acgggcgcag
     1501 tataaaaggc cgtgagcgct gcggactgtg cgccggtttc tgccgacgag gcaagacaaa
     1561 cgaaacagag cagtgagcag gctacgctga atctttgccg atgagacatt gcccagcctc
     1621 ctttcatctt cgatgcccgt tatcagtgac gcctcagcag cgtgcatttt cgataaatat
     1681 ccgccaaagt ctcgtcctgc tttgcatcgg attctcgtca agcgtgctta gctcttgccc
     1741 cacagtcgat caatgaaacc gctggttttc agttcagtaa aaaagcgcgg atcgaaaaaa
     1801 tcgtcggcct tagcagatgc cgcttgggga acggacttgc ggatcaaacc gaggatgagc
     1861 tcgatccctt tggggtcaac ttgcagggtg ggctccatcg catctccttc ggcggccatc
     1921 gagtgatgaa gcacctcttg gtcttcaacc ttggtgtatt tttgcacaat ctttgcggca
     1981 atctccggcc tggtcaaaaa cgtgtgaatc gcttcggcgt aggcgcggga gaatttggcg
     2041 atctcagcct gtttgcggct gacggtcgag cgggtcgaga gcagcacggt ggcggcaaag
     2101 ggaattttca acgccgtaat gtcgagaatg actttgaagc ccaagcgctg cgcaaagaga
     2161 tcatagggcg gggtggtggt aaaagcgtcg atggaaccgg ccttgagcgc gggaatgccc
     2221 tggctggtgc tgcccatttg cacgtagctc aaatccttca gcgggtcgat ttggaattgc
     2281 gccagcgctg cctgagtgta catatccgga ttggaaccaa tgcggtcgat gccgagccgc
     2341 ttgcctttga gctcttgcac gccggtgata tccttgcgcc ccacaatggc gccgaccatg
     2401 gcatgcttgc tttgtccgat gatcactaga tttttggcgc cgccgagaat cgcccgtatc
     2461 ggcgcgaagc caccgatgat acccacgtcg gtttcgccgg agagaatcga tgttgttcca
     2521 accggacccg agccgatgta aaccaggcgt acttcggcac cgtacttttt gaataaaccg
     2581 gtttctttag cgatccacat gggtgccatg gacaccacgt gagcggtata gaaggccgtg
     2641 agcgtcgaag actgagcaca gacaattgtg ctcaggctca tcgatgccat gaaggctatg
     2701 ccgagggtcg tcattcgctt gaattggaaa atcacgatga gcctccttac gcgcggggaa
     2761 tctgcaagct aaggtccggc tatgagacga ccactgcgcc tcgaacccaa tatcgcaacc
     2821 caggatggat gtccacaacg gctgcttatc gaacgcatta agcggacaat attgacacaa
     2881 gcgcgcgtaa ccactacttt cggcccgagc ctagggcggt attgccatct gttgttgtga
     2941 tttttggcgg accgattatg ggtgagagca gacggaaaaa ttggcgaatc gatgtgcatc
     3001 atcacgtcgt gccgcctagg tacgccgacg attccatgcc gattaaactg ccggatgccg
     3061 aagagcagct tggctcgatg gatcgctgga atattcgcgc ggcaatcact tcactcacgc
     3121 cgcgcgtcgt tttgaagaat ctcgatcggc tccgcagtgt ggcgcgggag tgcaatgaat
     3181 ttcaagctca gtgtgtccgc gaccatgcgg cgcgcctcgg cgctttcgct ttattgcctc
     3241 tacccgatgt ggatggcgct ttgcaagaaa ttgcttacgc gctcgacgtt ctgcgtctgg
     3301 acggcatcgg tcttttttcc agcatcacgc accgatatct cggcgatccg cagttcgatc
     3361 cggtgtttga cgagctcgac cgccgcggcg cggtggtgtt cattcatccg acccattgcg
     3421 aagctcccga ggacacacgt ctgcaggcgc caccctttgt tgtcgagtac gtgttcgata
     3481 ccacccgggc gatggtgaat ctgatttata cgggaacgct ccaacgttgt ccgaacattc
     3541 gcttcatcgt cgcccatggc ggcgggacct tgccgtttct cgctcaaagg gtcgcgatga
     3601 tggaaggtca tcgcggcggc aaaaatgtca ccggcgtgct gccggcgctg caatcgctct
     3661 attacgagat tgcgagcacg acttcaccct atgcgcttcg gtctttgcag gagcttgtcc
     3721 ggccgacaaa tatcctctgg ggttcggatc tgccgtttgt ctatggcgat cgattgcgtg
     3781 atgaagtcga gcattgggaa cattatacgg gctttgacac cgccggtcgt gcggcgatcg
     3841 aggccggaaa cgcccagcga ttgttttccc gatttgcgca gacggtatcg ggtgaacgcg
     3901 actcgatggt ttttttaaag gaggaattat gaggttactg aaatggcggc aatgggtggt
     3961 gctcagtttg atgatggtct tgccgctcgt ttccatcgcg cggttcacat cggccgccga
     4021 ttccggtcga tttctcgtca gctacggcgg cacggccggt tatcagctac cgctctgggt
     4081 taacaaggag ttcgggttca gcaagaagta tggcgtcgat ctggaaatca ttttgatcca
     4141 agcgggatcg ccgaatattc aggctttggt cggcggcagc ctgcaactga ctcaaaccgc
     4201 cgcgtcttcc gcggtgatcg gcgcatccca aggcgcaccg gtggtgattg tcgcgacgct
     4261 agaaaacaag ctgccgatgc tgctcatatc ccggccggaa atcaaagatc cacagcagtt
     4321 acgcggcaaa gtgatcggca tcaatcgctt cggcggctcc aatgatgcag ccgttctgat
     4381 ggcgatgaaa gcctggaaaa tcgacccgaa agagatttcc atgctgcaaa ccggcggcac
     4441 ctcggcgcgc atggcggcgc tgatcagcaa gaaagtcgat gcgacggtgc agtcctatcc
     4501 ggaaatttat caggcgcgaa agctcggcat gaacgtcttg gcggatatcg gcgatttcgg
     4561 tttttacacg aatacttcga acatcgtgac gcgctcgtac gttcaacaaa accgcgctgc
     4621 gatcaaggga ttcatcaaag gccagatcga aggaattcac tacgtcaaga cgaacaagga
     4681 aggcagcttg aaggttctgc gaaaacattt gcag
//
LOCUS       DASULZ010000339         2862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1362444, whole genome shotgun sequence.
ACCESSION   DASULZ010000339 DASULZ010000000
VERSION     DASULZ010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362444"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VFY96_07040"
     CDS             complement(<1..831)
                     /locus_tag="VFY96_07040"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5606252.1"
                     /translation="MIVDADAHVVETEHTWDFLDAAEKKFRPQLFSSTDNPNMQYWFV
                     DGKSVGFRPPTLTEQELIALSKQTGRELKTAPEARELRDVELRLKHMDKLGIDVQILF
                     NTMWIARVADKAEAEIALCKSWNRWMAEVWKQGNNRLRWSCVVPAMTLSEVVEQMRFA
                     KENGAVAVSLRPFEDEKHLVDPYFYPIYEEATRLNLAIAVHIANASPQLMDQFKPRYD
                     RMGGFAQFRVPTVITCLSLMMSDVPKNFPQLRWAFVEASAQWVPWVVKEAVRRSGTTG
                     F"
     gene            1237..2703
                     /locus_tag="VFY96_07045"
     CDS             1237..2703
                     /locus_tag="VFY96_07045"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX5606253.1"
                     /translation="MFDSSKIQPYISFTDLRKWMAEAEALGELRTVLGASWQQEIGLA
                     TDVVVPPDDGPAVLFDEVPGCPKGFRLLINCFAGKRRAMTLGFPQGLSKQELSQAYFD
                     HYQRTPKHIAPVIVESGPVFENSLKGADIDILKFPTPIWHVNDGGRYIGTGCFSVTMD
                     PDEKWVNAGCYRAMIQDQRSVSLLMVPGKHGYMHRQKYFKRGEKMPLALVLGGDPLFF
                     FMAGTEQPYGLCEYDIVGGMRRKPVECVRGKVTGLPFPANSEIVFEGYLNNDNRKFEG
                     PFGEWTGYYASDESAQPVLEIEAIYHRNDPVILGVPPIGGGSDEMARYRAIMRSAMLK
                     HQLQNAGVPDVTQVWPHEIGASRMLIGIAVKQRYPGHAKQVGVLAASCGASVYGCKMV
                     IVVDEDVDVSNLDQLIWAMLSRYDPATSVDVLRRMRSTPADPRLTPEQRKVRDFTNSR
                     MVIDATRPYEWRDKFPKVNAPSQEVVRQAREKFGYLLK"
BASE COUNT          631 a          793 c          805 g          633 t
ORIGIN      
        1 aaatcctgtg gtgccgctgc gccgtaccgc ttctttgacc acccagggga cccattgggc
       61 ggatgcctcg acgaacgccc agcgcagttg gggaaaattc ttcggcacat cgctcatcat
      121 caaggataag caggtgatga cggtcggaac acggaactgt gcgaagccgc ccatgcgatc
      181 gtagcgcggc ttgaattgat ccatcagttg cgggctggca ttggcaatat gcacggcgat
      241 ggccagattc agtcgggtcg cttcctcata gatcggataa aaatagggat ccaccagatg
      301 cttctcgtct tcgaacggcc gaagactcac cgctaccgcg ccgttttcct tcgcaaagcg
      361 catttgctcg acgacttcgc tgagggtcat agcagggacg acgcaggacc agcgcaggcg
      421 gttattgccc tgcttccaaa cttcggccat ccagcggttc catgacttgc aaagcgcaat
      481 ttcggcctcc gctttatcgg cgacccgggc aatccacatg gtgttgaata gaatctgtac
      541 gtcgatcccg agcttgtcca tgtgcttcaa gcgcagctcg acatcgcgca gttcgcgcgc
      601 ttccggagcg gtcttgagct cgcgtccggt ttgttttgag agcgcgatga gttcctgctc
      661 agtcaatgtc ggcggccgaa aacccaccga cttcccgtcc acaaaccaat actgcatgtt
      721 agggttgtcg gtggagctga aaagctgcgg ccgaaacttt ttttccgctg catccaaaaa
      781 atcccaggta tgctcggttt caacaacgtg ggcatcggca tcgacaatca tcgtggctcc
      841 tcaaacgctt gcgctactag gttttcctac gaatcagttt acgtcacggt ccccattcta
      901 tctaagctaa gcccgcggtc aaccgattgg agcgaagcat ctcgatctcg ataccccgaa
      961 ttgacttcgt ttcatccggg atcactgtca tttcggcgga aaggcgaaat ctttccgtgc
     1021 ccttctcctg agctaagaaa gatttctcgc tgcgctcgaa tgacaaaccg cgattagtca
     1081 aatgaaggta tgactctgcg atcagaatta ggtgccgcat cggcgacgaa ctgcgagatg
     1141 ttacctgatc aggcacgact cggatccaat ggctcctttt gccgctccga gtctttgtga
     1201 taggatttca cgatcgacag agaccaagga gaagccatgt tcgacagctc aaaaatccag
     1261 ccgtatatct cgtttaccga tctacgtaaa tggatggccg aggcggaagc gctgggcgag
     1321 ctaaggaccg ttctgggcgc atcatggcag caggaaatcg gtttggccac agatgtcgtc
     1381 gtgccgccgg acgacggtcc agccgtgctg ttcgacgagg tgcccggctg tccgaaaggt
     1441 tttcgcctgc tgatcaactg tttcgccggc aagcggcgcg ccatgacgct cggctttccg
     1501 caggggctct cgaaacaaga gctgagtcag gcgtatttcg atcattatca aaggacgccc
     1561 aagcacattg cgccggtgat cgtcgagagc gggccggttt tcgaaaactc gctcaaaggc
     1621 gccgatatcg acattcttaa atttcccacg ccgatttggc acgtcaacga cggcggccgg
     1681 tacattggca ccggctgctt ttccgtcacc atggatccgg acgaaaaatg ggtgaacgcg
     1741 ggctgctacc gcgcgatgat tcaagaccag agaagcgtta gcttgctgat ggttcccggc
     1801 aagcatggtt acatgcaccg gcaaaaatat tttaagcgcg gtgaaaaaat gccgcttgcg
     1861 ttggtgctcg gcggcgatcc gctatttttc ttcatggcag gaactgagca gccctatgga
     1921 ctgtgtgagt acgatatcgt cggcggcatg cgccgaaaac cggtcgagtg tgtgcgcgga
     1981 aaggttaccg ggctcccatt tccggccaat tcggaaatcg tctttgaagg ttatttgaat
     2041 aacgataatc gcaaatttga agggccattc ggcgaatgga ccggctatta cgcttccgat
     2101 gagagcgccc agccggtgct ggagatcgaa gcgatctacc atcgaaacga cccagttatc
     2161 ttaggggtgc cgcccatcgg cggcggctcg gacgagatgg cgcgttatcg cgcgatcatg
     2221 cgctcggcga tgctcaagca ccagttgcaa aacgccggcg tgcccgatgt cacacaggtt
     2281 tggccccatg aaatcggcgc gtcgcgcatg ctaatcggca tcgccgtcaa gcagcgctat
     2341 cccgggcatg cgaagcaagt cggcgttctg gctgcaagct gcggggcaag cgtttacggc
     2401 tgtaaaatgg tgattgtggt cgatgaggat gtcgacgttt ccaacctcga tcagctcatc
     2461 tgggcgatgt tatcgcgcta cgatccggcg acttccgtgg atgttctccg ccgcatgcgc
     2521 agcactcccg ccgatccgcg gctcacgccc gagcagcgta aagtccgcga tttcacaaat
     2581 tcgcgcatgg tgatcgacgc gacgcggccg tatgaatgga gagacaaatt tcccaaggtc
     2641 aacgcgccga gccaagaggt tgtccgccag gcgcgagaga agtttgggta tttattgaag
     2701 tgaggggatt gcgcccgtaa ccgagcaggt gcgagaaatc caagagagtt ttctcgctcc
     2761 gctcgaaatg acaaatccgc cgtagaagtc acaaacagta cgatctttgt agggcgcagc
     2821 atgctgttcc ctcggctctt taccaaatca tatccatgca gt
//
LOCUS       DASULZ010000340         2695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_144143, whole genome shotgun sequence.
ACCESSION   DASULZ010000340 DASULZ010000000
VERSION     DASULZ010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144143"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="VFY96_07050"
     CDS             complement(<1..910)
                     /locus_tag="VFY96_07050"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606254.1"
                     /translation="MKQIIMIAVLIFGVAIHGSDAQEKIRVGQGSISLQSGLMHIAKD
                     RGLFSKYGLVTETIYIPGGSTNIHVLISGNLDLSQLSGAPGVAANLEGADIIYFAGLL
                     DKLNYQLITRPEIKNVEQLKGRKFGVSRYGSSADFGMRAMLKKLGVDPVKDATILQIG
                     DEPSRIAAIKSGNIDGTVANAPFGIEAERLNLNVLADSLKMDIPFFNTGLLGSKRFLD
                     RQEAKVLNFLRAYLEAIKVLKTERDYSIKALAQFTRVQNLKAVQEGYDYFVKQLEPVP
                     YPSVSAMQAVVDQIAATNSKARGIDAK"
     gene            1071..1277
                     /locus_tag="VFY96_07055"
     CDS             1071..1277
                     /locus_tag="VFY96_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008359934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEX5606255.1"
                     /translation="MADVIIKTLKNGPLEIWGGAKLADEAGKEYAESGNEPLYLCRCG
                     HSRTKPFCDNSHEEIGFKAEETAD"
     gene            1392..2120
                     /locus_tag="VFY96_07060"
     CDS             1392..2120
                     /locus_tag="VFY96_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5606256.1"
                     /translation="MEGKVVIVTGGARGIGRHAAHTLAKAGAKIVLADSDLERMHKTL
                     AEIRNLDAEAIAVPTDVRDEKDVRRLVHETIDRFDQIDALINDAGIVPHFNWGIPRWP
                     RVRHLSKDFWDNVIQTNLGGTFLCTKYVVPFMEERRSGNIVNLWGGGRPENHGASPYV
                     VSKDAIRTFTRFVGEEEREWNVCVVAFSPKQAIATEDAPEEARKRLPGPEILGNGFVL
                     AAQAGMELTGKTVEHRDGKLVAVD"
     gene            2389..>2695
                     /locus_tag="VFY96_07065"
     CDS             2389..>2695
                     /locus_tag="VFY96_07065"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5606257.1"
                     /translation="MISISDQMKELVDNALANGSPCILATVSGDGEPDIGYKGSMMVF
                     DNESLAYWERTKRVHMKNVKENPRVIVLFRDAKTKAAWRFHGTATVHESGPIRDQVMA
                     "
BASE COUNT          654 a          704 c          742 g          595 t
ORIGIN      
        1 ttttcgcgtc gatgccacgt gccttggagt tcgtcgcggc aatctgatca acgacggctt
       61 gcatcgctga gacggacggg tagggtacgg gttcgagctg tttcacgaaa tagtcataac
      121 cttcctgcac cgctttgaga ttctgcacgc gagtgaactg ggctagcgct ttgattgaat
      181 agtcccgttc ggttttgagc actttgatcg cttcgagata ggcacgcaga aaatttagga
      241 ccttcgcttc ttgtcgatct aaaaatcgtt tgctgccgag caaaccggtg ttgaaaaaag
      301 gaatatccat cttgagcgaa tcggcgagca cattgagatt gagtctttcc gcttcgatac
      361 cgaagggcgc gttggcaacc gtgccgtcga tgttgcccga tttgatcgcg gcgatgcgcg
      421 agggctcgtc gccaatctgc aaaatcgtcg cgtccttcac cggatcgacg cccagctttt
      481 taagcatcgc gcgcatgccg aaatccgcag aagaaccata gcggctcacg ccaaattttc
      541 taccttttag ctgttccacg ttcttgattt ctggccgtgt gatcagctga taattgagct
      601 tatcgagcag cccggcgaag taaatgatat ccgctccctc aagattcgcg gcgacacccg
      661 gtgcaccgct tagctgggag aggtccagat tgccggaaat gagcacgtga atattggtcg
      721 acccgccggg gatgtaaatc gtttccgtca caaggccgta tttgctgaat agcccgcgat
      781 ctttggcgat gtgcatgagg cccgattgca aactgatcga gccctgccct actcgaattt
      841 tctcctgtgc atcagatccg tggatcgcca ctccaaaaat caagactgct atcattatga
      901 tctgtttcat ggcgcatctc ctaccataac gcgaactccc ctgccactgt gtcagcggcg
      961 tgatacgttt cagctaaatc acacaatcag atattttaaa gtgggcacac ggagcgttgc
     1021 gcagatctcc tctctgtgag atgttttcgc gagatgctcg gaggggaaac atggccgacg
     1081 tgatcatcaa aacactgaaa aacggtccgt tagaaatctg gggcggcgcc aagttagccg
     1141 acgaggcggg caaagaatat gccgagagcg gcaacgagcc gctttatctc tgccgttgcg
     1201 gtcattcaag aacaaaaccg ttttgcgata atagccacga ggagatcggc ttcaaggcag
     1261 aagagacggc tgactagaca aatcttccgc caatattcca ttactccaat acccagcacc
     1321 caatgggcgg ggtgattgga gtaatggagt ggtggagtga tgactatacg cagacctttg
     1381 ggaggcagct catggaaggt aaagtagtaa tcgtcactgg tggcgctaga ggcattggcc
     1441 gtcatgccgc gcatacgctc gcaaaagcag gcgcaaaaat cgttctcgcc gacagcgatc
     1501 tcgaacgaat gcacaagacg ctcgcggaaa tccgcaatct cgatgccgag gcgatcgccg
     1561 ttccaacgga tgtacgcgac gaaaaagacg tgcgccggct agtgcatgaa accatcgacc
     1621 gcttcgatca gatcgatgca ttgatcaatg acgccggaat cgtaccccat ttcaattggg
     1681 gcattcctcg ttggcctcgg gtgcgacatc tcagcaaaga cttctgggat aacgtgatcc
     1741 aaaccaatct cggcggaact tttctttgca cgaaatatgt ggtgccgttt atggaagagc
     1801 gacgctcggg aaacattgtg aatctatggg gcggcggacg gccggaaaac cacggcgcat
     1861 ctccgtacgt cgtcagcaaa gatgcgattc ggacctttac ccggtttgtc ggtgaagaag
     1921 agcgcgaatg gaacgtctgt gtggtcgcgt tcagcccgaa gcaggcgatc gcgactgaag
     1981 acgcgccgga agaagcgcgc aaacgtctcc cggggccgga aatcctggga aacggcttcg
     2041 tcctggcggc gcaggcaggc atggaattga ccggcaagac cgtcgagcat cgcgacggaa
     2101 agctggtggc ggtagattag cgaaaaaggc aactaggatt agtgatactc atcgttccca
     2161 ctccgaacga tgggtatcgg cgcgtgcgcc tccacccaca gtacaattta agatcagcta
     2221 aaacgaattc gagtggagaa gcgcacaccc ttcgttacgg cagcgttgcc gctgcctact
     2281 caggatgatc ggcggtacgg catcgagaga ttgccgttaa tcacctgaat gattcaattt
     2341 gaccgcgccc cacttcttct gtaattacta cgctccggga ggtgatttat gatttcgatc
     2401 agcgatcaaa tgaaagagct tgtcgacaac gcattagcca acggatctcc gtgcattctc
     2461 gccacggttt ccggtgacgg cgagccggat atcggctaca aaggcagcat gatggttttc
     2521 gacaacgaat cccttgcgta ctgggaacgt accaaacgag tgcacatgaa aaacgtcaaa
     2581 gaaaaccctc gtgtgattgt gcttttccgc gacgcgaaaa ccaaagccgc ctggcgtttt
     2641 cacggcacgg cgacggtgca cgagagcggt ccgatccgtg atcaggtcat ggcgc
//
LOCUS       DASULZ010000341         4195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_651842, whole genome shotgun sequence.
ACCESSION   DASULZ010000341 DASULZ010000000
VERSION     DASULZ010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651842"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1455)
                     /locus_tag="VFY96_07070"
     CDS             complement(<1..1455)
                     /locus_tag="VFY96_07070"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5606258.1"
                     /translation="MKNTSTTQTVPTAGIELAGTSTSWFRHVAILAGFCFLLLFVGLS
                     ELPFYNRGEPREGLVVWEMYSSGNWILPAVNGDYIPFKPPLFHWLALLVSHLFGGVDE
                     FTLRLPSALLATAGVLVIYQVGGRLWGQPAGIVAGVVLATCAEWWQAGTDTQVDMTLA
                     FFVSSACLYFDFLYHERDFNLLKALGLPLLLGLATLAKGPIGLALPGIIFLIFLCLRR
                     DFAFVQKLHPFLGGMVFLFVAGSWYAAALWQGGPAFFFRQIVDENIRTAAGTYGHHQP
                     VYYYLPIFLENSLPWSFFIPCMALFFYRRRHRLEEERLLFYLVWLFSVLTFFTASLGK
                     RGVYILPLYPAFALLFSAWWQQLEQNISNSASLARWMTYTFVTVCLAATFGIVLYYAA
                     QHGLIDQQQFVPLIKFKNVARVLSGLAGMSTYTTISVTLFLAGLGTLVWALFRRNWRF
                     TFAALSLMTLGVSTIVKTDLFLPVAFERTMKPFMV"
     gene            complement(1409..3331)
                     /gene="asnB"
                     /locus_tag="VFY96_07075"
     CDS             complement(1409..3331)
                     /gene="asnB"
                     /locus_tag="VFY96_07075"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX5606259.1"
                     /translation="MCGIYGFVCQRKEINPEILPRMGFALAHRGPDDGDERITISDAG
                     CVALGHKRLSIIDLSPAGRQPMPNEDKSIWLTLNGEIYNYQELRQELKSKGHRFRSHA
                     DSEVVVHLYEECGAQCLVKLKGMFAFALWDSKAKSLLLARDRIGKKPLHYCLTGDGLI
                     FASEIKALLQHPSVSRDLDFKALSKYLSYEYVPAPDTIFRSIKKLEPGHYLIYSNHHS
                     RVARYWDIPLQNNQTIRRSEEDCTEELRALLNLSVKRRLVADVPVGLFVSGGLDSGIV
                     AAFAAKAKSDLDCFSIGFDDPSFDESAYSRTIAASLGIRHHLKTFTGRDMLQLLEQLP
                     RLLDEPLADPSVFPLYLLSKFAAEHSKVVLSGDGGDELFAGYQTFQADKLMRYYGALP
                     GFVKHSIRTISQHLPVSHDYLSADYKIKQFLKADSVPPEIRFFLWRGAFSPADQYALL
                     APDIRAELGEYDCYEDIARYIDESGLGRGVERLLYLCMKLYLQDNNLVTVDRASMANG
                     LEVRCPLLDQDLVEFVSRLPARYKLKGFRAKYLLKKAAKGLLPETIIHRQKKGFGIPL
                     AKWLCTDLKDFMLDHLNEARIRRHGIFDYAYIKRLMDEQFAKIKDHRELLWTLIVFQS
                     WYEEYIDNPNRPNCRH"
     gene            complement(3441..>4195)
                     /locus_tag="VFY96_07080"
     CDS             complement(3441..>4195)
                     /locus_tag="VFY96_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606260.1"
                     /translation="YILPMFPSLAILVGITLGRILTARQAERPSPLAMSCLSLCLLVA
                     GFFAAGAFPHFFPPAAQFPFAEVSSLLRRTGLLIIAIGAAGLAVFTWSGRWKSRVSLF
                     VSLSIVLILYADLLGTILVGASMRRSTRDLAAKAAPYIQPDTQVVIYDTTLESLPFYL
                     KISRPIWGVWSGAKASVFGSVYLAEKAALSAPGFGRALLTFDEFKQEWAKAPKDHFAV
                     FIKRKNLPRLEQAVGHAATIRVERDGMLLVSN"
BASE COUNT         1121 a         1094 c         1100 g          880 t
ORIGIN      
        1 aaccatgaac ggtttcatcg tgcgctcgaa cgccaccggt agaaataagt cggttttgac
       61 gatcgtgcta acgccaagcg tcatcaaaga aagcgcagcg aaagtgaagc gccaattccg
      121 cctgaacagc gcccagacga gcgtaccgag accggctaga aacaatgtca cggagattgt
      181 tgtgtaggtt gacattcctg ccaacccaga tagaactcgg gcgacatttt tgaattttat
      241 cagtggaaca aactgctgct gatcgattaa gccgtgttgc gccgcataat acaggacaat
      301 tccgaatgtc gccgctaaac agacggtcac aaaagtgtag gtcatccagc gtgcgagtga
      361 agcactattc gagatgtttt gctcaagttg ctgccaccaa gcgctaaaaa gcagcgcaaa
      421 tgccggatag agcggtagga tgtagacgcc tcgcttaccg agcgaagcgg taaagaaagt
      481 caaaaccgaa aagagccaga cgagatagaa caataaacgc tcttcctcga gtctatgacg
      541 ccgccgataa aaaaagagcg ccatacaagg aatgaaaaag ctccagggta gtgaattctc
      601 caaaaaaatc ggcaggtagt aatagactgg ctggtgatgc ccataagtgc cagcagcggt
      661 cctgatattt tcatcgacga tttgccggaa gaaaaaagcc ggcccgcctt gccagagtgc
      721 cgccgcatac cacgaccctg caacgaaaag gaaaaccatt ccgcctaaaa acggatgcag
      781 cttttggaca aacgcgaagt cgcgccggag gcaaagaaaa atcaaaaaaa taattcctgg
      841 cagggccaat ccgatagggc ctttggcgag tgtggccaat cccaaaagca aaggaagtcc
      901 caaagctttc agcaagttga aatcgcgctc gtggtagaga aaatcaaaat acaagcaagc
      961 cgagctcaca aaaaaagcca atgtcatatc cacctgcgta tcggtgccgg cttgccacca
     1021 ttcggcacag gttgctagaa ccacgccggc tacgatgccg gcaggttgtc cccacagtcg
     1081 cccgcccact tggtaaatca ccagcacacc cgcagtcgct agcaatgccg aaggcaagcg
     1141 cagcgtaaat tcgtccaccc caccgaaaag atgacttaca agcagtgcga gccaatggaa
     1201 tagaggcggt ttgaagggga tgtagtcgcc attgacggcg ggaagaatcc agtttccaga
     1261 ggaatacatc tcccagacca ccaggccttc acgtggttcg ccgcggttgt agaacggcag
     1321 ctcgctcaat ccaacgaaca gcaggaggaa gcaaaaaccg gcaagaatcg caacatggcg
     1381 aaaccagctg gtactcgtgc cagcgagctc aatgccggca gttgggacgg tttgggttgt
     1441 cgatgtattc ttcataccaa ctctgaaaaa cgatgagagt ccacaacagc tcacggtggt
     1501 cttttatttt ggcaaattgt tcatccatga gccgcttgat gtaagcgtag tcaaaaattc
     1561 catgtcttct gattctcgcc tcgttcaaat ggtcgagcat aaaatctttg agatccgtgc
     1621 agagccattt ggccagcgga ataccgaaac cctttttctg ccgatgaatg atagtttcag
     1681 gcagcaaacc tttcgcggcc tttttgagca gatatttcgc tcgaaaaccc ttgagcttgt
     1741 atctcgcggg taagcgggaa acgaattcga caaggtcctg atcgagtagc ggacagcgaa
     1801 cttccaaacc gttggccatg ctggcgcggt ccaccgtcac gagattattg tcctggagat
     1861 acagtttcat gcaaagataa agcaggcgtt caacacctct tcccagtccg ctttcatcaa
     1921 tgtaacgagc aatgtcctca tagcaatcat actcgccgag ctcggcgcga atatccggag
     1981 caagaagggc gtattggtcc gccggtgaaa aggcgccgcg ccacaagaaa aagcggattt
     2041 ccggcgggac gctatcagcc ttcaaaaatt gcttgatctt gtagtccgcg cttaaataat
     2101 cgtgtgaaac cggcaagtgc tgcgagatgg tccggataga atgcttgacg aaacccggta
     2161 aggcgccgta gtaccgcatt agcttgtcgg cctggaaagt ttgatacccg gcaaaaagct
     2221 catcgccgcc atccccgctg agtacgacct tggaatgttc tgcggcaaat ttagaaagca
     2281 gatagagcgg gaaaactgat ggatcagcga gcggttcgtc aagcagccgc ggcagctgtt
     2341 ccagcaattg cagcatatcc cggccagtaa acgttttgag atggtgacgg ataccgagac
     2401 ttgcggcgat ggtccttgag taggcgcttt catcgaagct agggtcatcg aagccgatgg
     2461 aaaaacaatc cagatcgctt ttcgctttcg ccgcaaacgc tgcgacgatc ccggagtcta
     2521 gtccaccgct gacgaagagc cccaccggca cgtcggcgac cagtctgcgt ttgacactga
     2581 gattcaaaag tgccctcaat tcttcggtgc agtcttcttc gctccggcgt attgtctgat
     2641 tgttctgtag gggaatatcc cagtatcgtg caacgcggct gtgatggttg ctgtaaatca
     2701 ggtagtgacc gggctccagt ttcttaatcg agcgaaagat tgtgtcgggt gcgggaacat
     2761 attcatagct gagatatttg ctgagcgctt tgaaatccaa atctcggctt actgaaggat
     2821 gctgcaaaag agcttttatc tctgaagcga agattagacc atcccctgtg agacagtagt
     2881 gcaggggctt tttaccgatg cgatcacggg ccaacagcag ggatttcgct ttgctgtccc
     2941 acagagcgaa agcgaacatg cccttgagct tgaccaggca ttgggctccg cactcttcgt
     3001 acagatgcac gacgacttcg ctgtccgcat gggaccggaa ccggtgccct ttgcttttca
     3061 gctcttgtcg gagttcttga taattgtaga tttcaccatt gagcgtgagc cagatggact
     3121 tgtcctcgtt cggcattggt tgtcgccccg cgggagacag atcgatgatg ctcagccgtt
     3181 tgtgtcccaa cgcaacgcaa cccgcgtctg agatggtgat cctttcatcg ccgtcatcgg
     3241 gaccacggtg tgccagcgcg aatcccatgc gaggcagtat ttcaggattg atttctttcc
     3301 tctggcatac gaaaccgtaa atcccacaca tcgctaatta tctttgacag cagtttgaca
     3361 gaatcccgtg tgccggtaga ttgactcctt gttgccccca gcgacccgct aacggaccac
     3421 aggcgaaaaa gctgtttagg tcaattcgag acgagcagca tgccgtcgcg ttctacacgg
     3481 attgtcgcag cgtgccccac tgcttgctcc aaacgcggaa gattttttcg cttgataaaa
     3541 accgcgaaat gatccttggg cgccttcgcc cactcttgct tgaactcgtc aaaagtcagc
     3601 agcgcgcggc cgaatccagg cgccgacaac gcggcctttt ctgccaggta aacactgccg
     3661 aaaacgctag ccttcgcgcc agaccaaaca ccccatatcg gacggctgat ttttagataa
     3721 aaaggcaggc tttcaagggt ggtatcgtag atgacgacct gagtatctgg ctgaatatag
     3781 ggagccgctt tggcggccag atcccgtgtc gagcgacgca tcgaagctcc aaccaaaata
     3841 gttccgagca gatccgcgta cagaataagg acaatgctca acgacacaaa caacgagact
     3901 cggctcttcc atcgtccgct ccacgtgaat acggcaaggc cggcagcacc gattgcgatg
     3961 atgaggagcc ccgttcgccg caggagcgat gagacttcgg caaatggaaa ttgcgccgca
     4021 ggtggaaaaa aatgaggaaa tgcgccagcg gcaaagaaac cggcaactaa caggcacaga
     4081 gacaaacaag acatcgccaa tggcgatggc cgctcagctt gtcgagccgt caagatgcgg
     4141 cccaaagtta ttccgacgag gattgccaga gatgggaaca tgggaagaat gtagt
//
LOCUS       DASULZ010000342         3653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1159332, whole genome shotgun sequence.
ACCESSION   DASULZ010000342 DASULZ010000000
VERSION     DASULZ010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159332"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(286..1626)
                     /locus_tag="VFY96_07085"
     CDS             complement(286..1626)
                     /locus_tag="VFY96_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015145394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX5606261.1"
                     /translation="MFYVLVILFMILANGFLVMAQTALVAAHKSQLQDWVSRGNRRAK
                     IALRLSAEPNRVLAAIQASIRFLMIVSGVLAGRSLVEPLTDRLVQVPLLGGYGGGVSL
                     GILVVVLSYLSLSLGELIPRRIASLYPETIAVLMARPLELLTRISWPITRLLELSTEL
                     GSRLFGRERVSNPQITEDEIKSLVKQGTEAGVFEETEQDMLEAVLRLGDKRARSLMTP
                     RTQIAWLDRDDSNERIREKIIASGHSCFPVATGSLDKVDGVVQAKDLLAHSLAGQGLD
                     LGSQMQQPLFVPRTITALEILESFKNSGQHIALVVDEYGGIEGLLTHHDILEAIAGDI
                     PFDGRPNDPKAVRRHDGSWLLDGMLSIDEFKEILQIEVLPGEKRDAYQTLGGFVFTKM
                     GRIPSVSEHVEWNGLRFEVVDMDGKRIDKVLVSPLEIEARPAVGQNSDSSMREK"
     gene            1859..2928
                     /locus_tag="VFY96_07090"
                     /pseudo
     CDS             1859..2928
                     /locus_tag="VFY96_07090"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
     gene            2921..>3653
                     /locus_tag="VFY96_07095"
     CDS             2921..>3653
                     /locus_tag="VFY96_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX5606262.1"
                     /translation="MSESAEIGSTQILHGHKLGSVLEVLSVSTRLGLTSFGGPIAHLG
                     YFHEEYVKRRKWIDEQSYADLVALCQFLPGPASSQVGIAIGIARARLAGGVAAWLGFT
                     MPSALALIAFALGVGTFASAADTGWLHGLKVVAVAVVAQAVWGMARSLCPDRERATIA
                     IIASLVTLAWPTAIGQLSSIAIAGIVGAISFPSAASPPVSHMRFPISKKTGIAAWTIF
                     FTLLLGLPLLRQLLTSHALEVFDSFF"
BASE COUNT          844 a         1046 c          950 g          813 t
ORIGIN      
        1 gtcggaccct tgtgttttcc cggaacaatc tcggtaatat cgtgagccag cggcaaaccc
       61 acggcttctt cgaccggtat cactttaagc atgcaatttg atttgcctcc cgaaccgaat
      121 ttgctaattg cggtgatttc attctactca ccgccctcgc gctttgtcca gcaatccaac
      181 gcagcgcaag atacgcaggt ttaatgggtc aggatcgact gggccggtcc tctgaacttg
      241 gcttataaga gaatcacggc cggcccgttg ttgcacgtct cgtgattact tctcccgcat
      301 cgacgaatca ctattttggc cgacggctgg acgcgcttct atttctaacg ggcttaccag
      361 aactttatcg atgcgtttgc cgtccatatc gacgacctca aatcggagcc cgttccattc
      421 gacgtgttcc gacacggatg ggatcctccc catcttggta aacacaaagc caccgagggt
      481 ctgataggcg tcgcgcttct cgcccggcag tacttcgatt tgaagaatct ctttgaactc
      541 atcgatgctg agcatcccat ccagcagcca ggagccatcg tggcggcgta ccgctttggg
      601 atcattgggc cggccgtcga aaggaatatc cccggcgatc gcttccaaaa tatcatgatg
      661 agtgagcaaa ccctcgatac cgccatattc atccacgacc agcgcgatgt gctgtcctga
      721 gtttttgaat gactctagaa tctcgagagc tgtaatcgtt cgcggcacaa acaatggttg
      781 ttgcatctgc gacccaagat ccaggccttg tccggccaag ctgtgcgcga gcagatcctt
      841 cgcctgcacg actccgtcaa ctttgtcgag actgccggtc gccaccggaa aacaggaatg
      901 gccgctggcg ataattttct cccgtatccg ctcgttgctg tcatcgcgat ccaaccaggc
      961 gatttgagtt cgcggcgtca ttaatgatcg cgcccgttta tcgcccagcc gcagcaccgc
     1021 ctcgagcatg tcttgctcgg tttcctcgaa tacacctgct tcggtgcctt gcttgacgag
     1081 actcttgatc tcgtcctcgg taatctgtgg gttgctgacc ctctcgcggc cgaacagacg
     1141 cgatccaagt tccgtagaca actccagcaa tcgcgttatt ggccatgaaa tgcgagttaa
     1201 caactctagc ggcctcgcca tcaacaccgc aattgtttcg ggataaaggg acgcgatgcg
     1261 tcgcgggatc agttcaccca aactcaaaga aagatagctg agcacgacaa ccagaatgcc
     1321 taaactgact cctcccccgt atcccccaag tagcggcacc tgaacgagcc gatccgtaag
     1381 cggttcgacc aagcttcgcc cggccagtac gccgctgaca atcattaaaa atctaatact
     1441 cgcctggatc gccgcaagca cgcgattagg ttcggcactc aatcgtaacg caattttggc
     1501 gcggcgattg cctcgtgaga cccagtcctg caattgcgat ttgtgcgccg cgaccagtgc
     1561 ggtctgcgcc atcaccaaaa agccattggc cagaatcata aataatatga ccaatacata
     1621 gaacatggcg tgtcctcgtg gcgcctgaga ttgggggagc gcatgactct ccggtcgtgg
     1681 acccatcatg gctatcccag gcaccaccct tacgggggtg atcatacaaa gggtacgaat
     1741 ttaaggaaag ctaccttgca gtaagcccgg aacggagtta tgtcgaatcg aacctagatt
     1801 gacggcaagt tccgccggtt cgttgccatc cgacaagtgc aaaatttcat cctggaggat
     1861 gtacgaagaa attaatcgtc aaaacagcct tcaaaacttt cggtcaagaa gtcgacgtgg
     1921 ttttgatcgc gcttacagtg ttggtttgcc aaagcccaga tcccgcaggc gctcaaagat
     1981 catcgacgaa tggacgagcg tacaaccacc caagccgccg gagttgaaac cagtgacgct
     2041 cgatccaaaa acgaccgcgc tactggtgct cgatttcgtc aagcagactt gcaacgccga
     2101 acggcggcct cgatgcctca cgtcgctgcc caaagttcag gctctgttga aactcgcccg
     2161 agataaggga gtgacagtcg tttacaggat cacgaccgca tcgactccgg aggatatcgc
     2221 taaggaacta acgcctcaac aaggcgagcc gatggttaaa gcgcccgcag acaagttttt
     2281 caagacggac ttggagaaaa tcctcgcgga caaggcatca aaaccgtcgt cgtggtcgga
     2341 acggcagctc agggagcggt gctcaatacg gccagccaag ctgctttccg tgggttacag
     2401 gttattgtcc ctcgggacgg aatgtcggcg gaaagcacgt attatgagca gtacaccgtt
     2461 gttcatctga caacgtctcc cggcgtgggc cagcaggtga ccatgacaaa aatcgatctt
     2521 tcaaataagc ccacagaaag gaggacgaca atggctcggg tagcgcgagc tcaaatgaat
     2581 caacttgcgg cgctgcaaaa agattttcag cgactgcaaa aaagattgca cgcgatccat
     2641 aaaaaaaccg gctacgaaga tctcgcccat ggtgtgcttg cgctggaaat tgctgagcac
     2701 acagtggagg agacactgga gcacactgga cttggcggtg agatcgagca ccagccggat
     2761 aagaaaggcc accgtcaggc aaagcagtgg cttaaaacgg tcaagggaat tcaaaatcag
     2821 gccaacaaat ttcttagaac gcatccgaac gaggatttgg aaaccgccct gaaagccttg
     2881 gctatcgctg acggttccct gaaggaagtc gccgagcatt atgagtgagt cggcggagat
     2941 cggctcgact caaatcctgc acggtcacaa actcggttcc gtcctggaag tcctttcggt
     3001 ttcaacccgt ctgggtctta cttccttcgg cggcccgatc gctcatctcg gctactttca
     3061 cgaagagtac gtcaagcgtc gtaagtggat cgatgagcaa agctatgccg acctcgtcgc
     3121 gctttgccaa ttccttccgg gaccagccag cagccaagtc gggattgcca tcggcattgc
     3181 tcgcgcacgc ttagctggcg gcgtcgccgc gtggctgggc tttaccatgc catcggccct
     3241 ggcattgatc gcattcgcct tgggcgtggg cacgtttgcc agcgccgcgg ataccggttg
     3301 gctgcacgga ctgaaggtcg tcgctgtggc cgtggtcgcc caggcagtct ggggcatggc
     3361 ccgatcgctt tgccccgacc gcgagcgcgc cacaatcgcg attatcgctt cgcttgtcac
     3421 cctcgcctgg cccactgcca ttggacagct ttcttccatc gcgattgcgg gcatcgtagg
     3481 ggcgataagc tttcccagcg cagcgtctcc tccggtttct catatgcgct ttcctatcag
     3541 caaaaaaacc ggcatagcgg cttggacgat tttctttacg ctcctgttag gattgcctct
     3601 gttgagacaa ttgttgacca gccatgcgtt agaagttttc gatagttttt tcc
//
LOCUS       DASULZ010000343         2358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1362782, whole genome shotgun sequence.
ACCESSION   DASULZ010000343 DASULZ010000000
VERSION     DASULZ010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362782"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VFY96_07100"
     CDS             <1..439
                     /locus_tag="VFY96_07100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX5606263.1"
                     /translation="HFATGVTVITTVDRDGNPNGLTANAFLSLSLNPPLVLISVDKAA
                     TCYACFGLENGFTVNFLSEDQEEISRRFATKGADKFADLNWRSGTNGAAIIDGVLGYV
                     ECKIRECHDGGDHTIVVGEIVNVQATGDRPLLFFKGKYQRLPY"
     gene            complement(468..1445)
                     /locus_tag="VFY96_07105"
     CDS             complement(468..1445)
                     /locus_tag="VFY96_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606264.1"
                     /translation="MTVNGNGDQALHLATHSGWYRFEPRGTDWIQADRALTYWQMSCI
                     QVDPQDPKRVYIGTEHSGMFVSRNGGEDWTRANPNVPCLTMSSMLALPGKLLAGTMPA
                     ALYLTANGGAWQELPGVRHGSIGGCFPPNPDFPPRTRVLKKSEQNGGRLFAGIEVAGI
                     LVSDDDGQTWLPANDGLTDPDIHQILPCRHRAGLVVAACGEGVSRSTDGGRHWHKVTP
                     AGGRTYGNALTEDASGTIYLGITRDRPRTWTRAGRAQSAVFKSQDGARWELLTEEMAG
                     GVMDLCRAPDGESVFVSTSDGEVVELCATGESRTIISGLPCITAMALGA"
     gene            1566..>2358
                     /locus_tag="VFY96_07110"
     CDS             1566..>2358
                     /locus_tag="VFY96_07110"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HEX5606265.1"
                     /translation="MNFWAKIPKPIIGLSPMDGVTDASFRFITAKHGKPDVTLTEFVN
                     IQSALHSPQTLLKDLTYSEIERPIVAQIYGKTPELFYQVAHVVCELGFDGLDINMGCP
                     ARKVAATGCGAALIRTPTIAREIVRAAHRGIQDWYHGQMLKDLNLRPDLIQGIHELNL
                     ARNGWKTPPQRRRIPVSVKTRLGYDRVIVEDWLGVLLEEQLAVISLHGRTLEQGYRGK
                     ADWKAIARAVEIVRPTDTLLIGNGDAQDLSDVYRRVRQTGVDGVLV"
BASE COUNT          519 a          671 c          664 g          504 t
ORIGIN      
        1 acatttcgcc accggtgtca cggtgattac caccgtcgat cgagacggca atcccaacgg
       61 gctcactgcc aacgcctttc tatcactgtc gctcaatccc cctctggtcc tgatcagcgt
      121 cgataaagcc gccacctgct atgcatgttt tggtttggaa aacggcttta cggtgaactt
      181 tttaagcgaa gatcaagaag aaatttcgcg ccggttcgcc acgaagggcg cggacaagtt
      241 tgccgactta aactggcgca gcggtacgaa cggagcggcg attatcgacg gtgttctcgg
      301 ctatgtcgaa tgtaaaattc gagaatgtca tgacggcggt gatcacacca tcgtggtcgg
      361 cgagatcgtc aatgtacaag ctacgggaga tcgaccgctc ctcttcttca agggcaaata
      421 tcagcggctg ccatattaat tggatatctt gccgcggaaa aaccgagtta ggctcccagc
      481 gccatcgcgg tgatgcaagg taggccgctg atgatcgtcc gactttcgcc ggtggcacaa
      541 agctcaacga cctcaccgtc ggaagtcgag acgaaaacgc tctcaccgtc tggcgcgcga
      601 caaagatcca tgacgccgcc cgccatttcc tccgtaagta attcccaacg ggcgccgtcc
      661 tgcgatttga ataccgcgct ctgcgcgcgg cctgcgcgcg tccaggtgcg cggccgatct
      721 cgagtaatgc caagataaat cgttccgctt gcatcttccg tcagagcatt gccataggtg
      781 cgcccgcccg cgggggtcac tttgtgccag tggcggccgc catcagtgct ccgagaaact
      841 ccctcgccac aagctgctac gaccagaccc gctctgtgtc tgcagggcag aatttggtgg
      901 atatccgggt ccgtcaatcc atcatttgcg ggcagccaag tttgaccatc atcatcgctc
      961 acgagaatgc cggcaacttc gatgccggca aaaaggcggc caccgttctg ctccgacttt
     1021 ttaagcacgc gggtgcgagg cggaaaatca ggattgggag gaaagcagcc gccgatcgaa
     1081 ccgtgacgca cgcccggtag ttcttgccat gcgccgccat tcgctgtgag gtagagggcc
     1141 gccggcatgg ttccggcaag caacttgcct ggaagagcga gcatcgaaga catcgtcaga
     1201 cagggaacat tcggattggc ccgtgtccaa tcctctccgc cgttacggct aacaaacatt
     1261 ccggaatgtt ctgtgccgat gtaaacacgt ttaggatcct gtggatcgac ttgaatgcag
     1321 ctcatttgcc aataagtgag ggcgcgatcc gcttgaatcc agtcggtgcc tcgcggttcg
     1381 aatcgatacc aacccgaatg ggttgcaaga tgaagcgcct gatcaccgtt tccgtttacc
     1441 gtcatgaaat gcctccccat agccctggtg attcgaacat agttgccgcg cgatcgtagg
     1501 tcaaggccat gggacgagca cgctcgcttg gccgtgttcg cagtgctatg ttggtccgcg
     1561 ataggatgaa tttttgggcc aaaataccaa aaccgatcat cggtctctcg ccgatggatg
     1621 gcgttactga tgcgtcattc cggtttatca cggcaaagca cgggaaaccc gacgtgaccc
     1681 tgaccgaatt tgtcaatatt caatcggccc tccattcacc gcaaaccctg ctcaaggatc
     1741 ttacctacag tgaaatcgag cggccgatcg tggcgcaaat ttacggcaag actccagagc
     1801 ttttttatca agttgcccac gtcgtgtgtg agcttggttt cgatgggctc gacatcaata
     1861 tgggttgccc ggcaaggaaa gtagccgcca cgggttgcgg cgccgcactc atccgcacgc
     1921 cgacgatagc gcgtgagatc gtgcgcgccg cgcaccgagg cattcaagat tggtaccacg
     1981 gccaaatgct caaagacttg aatcttcgcc ccgacctgat ccagggcatc cacgagctga
     2041 atttagcgcg caacgggtgg aaaacgccgc cccaacggcg tcggattccg gtttcagtaa
     2101 aaacacgctt gggatacgac cgggtgatcg tggaagactg gctcggtgtc cttttggaag
     2161 aacagttggc ggtgatctcg cttcatggcc ggaccttgga acaaggctat cgcggtaagg
     2221 cggattggaa agcgatcgcg cgtgccgtgg aaatcgttag accgacggat acgctgctga
     2281 tcggcaatgg cgacgcccaa gatctctcgg atgtttaccg gcgcgtgcgt cagaccggcg
     2341 tcgatggagt gctcgttg
//
LOCUS       DASULZ010000344         4831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_43969, whole genome shotgun sequence.
ACCESSION   DASULZ010000344 DASULZ010000000
VERSION     DASULZ010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43969"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VFY96_07115"
     CDS             <1..489
                     /locus_tag="VFY96_07115"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5606266.1"
                     /translation="RRVYAQPFEQMLGLGSFIGGGILERHPKIKVAFLEANCSWLPWL
                     LWRLDEAYEREGDIFMPDLRMPPSAYFKRQCFISVEPDEAPARHTIEEIGCDHLVFST
                     DYPHGDSKYPHAVESFLTLSLSDEDKHKILWDNCARFYGAAESVSQSKTADAKSTQPL
                     QA"
     gene            498..1139
                     /locus_tag="VFY96_07120"
     CDS             498..1139
                     /locus_tag="VFY96_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606267.1"
                     /translation="METQSEIQSRSEIASENPMERFIRKTRALFAQESDLDKRWNALR
                     PILAELLADPEVIAASKRWPDCVPANGRAENLLFYQDPDYGFAMNGLTKGEARHGQRA
                     RIHDHAHIYTLYGVLDGRERVIRYDRLDDGSKPNYAEIREASNVLVGPGEIDLVKPYE
                     IHTELTVGERTVAVIIRSQKGGDFNQGRYIPEKNEYYESLGPRQTPCEMLPKD"
     gene            1175..2011
                     /locus_tag="VFY96_07125"
     CDS             1175..2011
                     /locus_tag="VFY96_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008924409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HEX5606268.1"
                     /translation="MSRVEPAEKTRPVLEALINYSVDVGIKPASYGGINTFEADKKRT
                     GKYQEHKVPIYDGRAIANSLSLEREGFMLVDHHTRVKDFYDEAEIRAVYYPEIEQLVK
                     ETSGAKRVLVFDHTLRSADSAMREAKQISGPVRNAHNDYTEWSGPQRVRDLLPDEAEE
                     LLERRFAVVQVWRPIRHAVEREPLAIADARSIGTKELFPSSRVYPDRVGEVYHCAYNP
                     EHRWYYFPKMQRNEAIVFKTFDSAKDGRARWTAHSAFDDPSSPPDAPPRESIEMRTLA
                     FF"
     gene            complement(2008..3084)
                     /locus_tag="VFY96_07130"
     CDS             complement(2008..3084)
                     /locus_tag="VFY96_07130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX5606269.1"
                     /translation="MTNDFVEYWAAAKLLLAGGNPYSPTELLNMERSVGWPQSEPLVM
                     WNPPWTLPLISPLGMLDYDTAQFIWFLFHSLIIFVGAQILWRSYTDEPKNARYGLLPV
                     LVFPPIYFALLLGQIGPVILLGILGFLFFVQRRAWFFAGASLSLVSIKPHVSYLLWIA
                     LVFWVLKERVWQLAISAALAELLLVLTPLWWNSAIYTQYAELLSSHAVVQPTEWATPT
                     LGTALGVLSGNPGGAMRWLPSILGALWLLWYWSHHRTGWNWIIETPLILLVSVATASF
                     AWTFDHVVLLPALVQCAVWMGRKNLATQQRYIVIGAYLGLAAVLVLGKTIGLNDFWSF
                     WYAPMLLLLYLYAWSHLRVGRECR"
     gene            3196..4809
                     /locus_tag="VFY96_07135"
     CDS             3196..4809
                     /locus_tag="VFY96_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX5606270.1"
                     /translation="MESQSKPNFLLIITDQHRADHLGCYGNPIVRTTNIDRLASTGTR
                     FDRFYVATPICMPNRATLMTGRMPSLHGARQNGIPLSLTATTFVEILRAAGYDTALIG
                     KCHLQSISGSLPTIGMPIVDLTKTPPPAHLREADRSWLTHGRYDQELRSTWINNADFE
                     LALPYYGFDHVELAVGHGDQVVGHYDRWFKQRCADADSLRGARNQLPGNNYIAPQAWR
                     TKVPEDLYPTAYVAERTMMYLENYARTDRSRPFFLQCSFPDPHHPFTPPGRYWDMYDP
                     TAVTLPRSFNSAERPIPPHLQALYEERSQRAANKDGQRTFAITEREAREAIALTYGMI
                     SMIDDAIGSILGWLKMLRLDEDTVIIFTSDHGDFMGDHQLLLKGALHYRGLVRVPFIW
                     SDPESESKGYVNTGLCGTLDIARTVLSRAGLQGHNGMQGLSLLPAVHGETTGHDALII
                     EEHQRRGYMGLQNNFRARSLITGDLRLTLYEGVEWGELYDLGKDPDELNNLWDNPQWR
                     DRRYQLTERLARKMMELADSSPLATHHGP"
BASE COUNT         1216 a         1363 c         1279 g          973 t
ORIGIN      
        1 cggcgcgtct atgcgcaacc cttcgaacaa atgctgggcc tcggcagctt cattggcgga
       61 ggcatactcg aacggcatcc taaaataaaa gtcgcttttc tcgaagccaa ctgctcatgg
      121 ttgccgtggc tgctttggcg gctggacgaa gcgtacgagc gcgagggcga tatctttatg
      181 cccgatttaa gaatgccgcc gagcgcgtac ttcaaacgcc agtgcttcat ctccgtcgaa
      241 cccgatgaag cgccggcccg ccataccatc gaagaaatcg gctgtgatca tctggtcttc
      301 tcgacagatt atccgcacgg cgattccaaa tatccgcacg cggtagagag ctttttaacg
      361 ctttctttat ccgacgaaga caaacacaaa attctttggg acaactgcgc gcgcttttac
      421 ggcgccgctg agtctgtgag ccaaagcaaa acggcggacg caaaatcgac gcaaccactg
      481 caggcatagg aggaactatg gagactcaaa gcgagattca atcccgttcg gaaatagcga
      541 gcgaaaaccc aatggagcga ttcattcgca aaacccgggc gcttttcgcc caagaatccg
      601 atctcgacaa acgctggaac gcgctgcggc cgatcctggc ggaattgctc gcggaccccg
      661 aagtcatcgc tgcttccaag cgatggccgg attgcgtacc agcaaacggc cgcgccgaga
      721 atttactgtt ttaccaagat ccggactacg gctttgccat gaacgggctc accaagggag
      781 aggcacgaca tggccagcgc gcacgtattc acgatcatgc gcacatctac actctttacg
      841 gcgtgctcga cggccgcgaa cgagtcatcc gctatgatcg cctcgatgac ggttcgaagc
      901 cgaattatgc ggagattcgc gaagcgagta atgttctcgt cggcccggga gagatcgact
      961 tggtgaaacc gtacgaaatt cacaccgagc ttaccgtcgg tgaacgcacc gtcgccgtga
     1021 tcattcgcag ccaaaagggg ggtgatttca accaaggccg ctacatcccc gaaaaaaatg
     1081 aatattacga aagcctcggc ccgcgccaaa cgccctgtga gatgctgccg aaagattgag
     1141 cgaaaccaac aggcgagaaa tgagcggtga atccatgtcg cgagtcgaac cagccgaaaa
     1201 aacacgcccg gtccttgaag cgctgattaa ttactccgtc gacgtcggta taaagccggc
     1261 aagctacggc ggcatcaaca cgttcgaagc cgataaaaag cgtaccggca aatatcaaga
     1321 gcacaaagtg cccatctatg atgggcgagc gattgcgaat agtctttccc tcgagcgaga
     1381 agggttcatg ctcgtcgatc accacacgag ggtgaaagac ttttacgacg aagcggaaat
     1441 tcgtgcggtt tattatccgg aaatcgagca actagtaaaa gaaacgtcag gcgccaaacg
     1501 agtcttggtc ttcgatcaca cgcttcgttc cgccgacagc gcgatgcgcg aagccaagca
     1561 gatcagcggt ccggtacgca atgcgcacaa tgactatacc gaatggtccg gcccacaacg
     1621 cgtacgcgat ctgctccccg atgaagccga ggagcttttg gagcgtcgct ttgcggtcgt
     1681 gcaggtctgg cgtcccattc gccatgccgt ggaacgagag cccttggcga tcgctgatgc
     1741 gagaagtatc ggaaccaaag aactgtttcc aagctcacgc gtctacccgg accgggtcgg
     1801 cgaagtttac cactgcgcct acaatccgga acaccgctgg tactacttcc caaagatgca
     1861 gagaaacgag gcgattgtgt tcaaaacgtt tgattcggcc aaggatggcc gcgcccgctg
     1921 gacggcgcat tcggcattcg acgatccttc cagcccgccc gatgctcccc cacgtgaaag
     1981 tatcgaaatg aggaccctgg cttttttcta acgacactct cgaccgaccc ggaggtggct
     2041 ccacgcgtat aaatacagca gcagaagcat cggcgcatac cagaaagacc aaaaatcgtt
     2101 gagaccaatc gttttgccca aaaccaatac tgccgcaagt cctaggtacg caccaatgac
     2161 gatgtagcgt tgctgcgttg caagattttt tctccccatc cacaccgcgc actgaaccag
     2221 tgccggtaat agaacaacgt ggtcgaacgt ccacgcaaag cttgccgtcg caacggaaac
     2281 aagaagaatc agcggcgttt cgataatcca gttccagcct gtccgatggt gcgaccaata
     2341 ccaaagcaac caaagtgctc ccagaatgga tggcaaccag cgcatcgcac cgcccggatt
     2401 tcccgataga acgccgagcg cagtgcccag cgtcggtgtc gcccattcag ttggctgcac
     2461 gacggcatga ctgctcagta gctctgcgta ctgtgtataa attgcggaat tccaccataa
     2521 aggcgtaagc acgagcagca attcggcgag cgctgcgctt atggccagct gccaaacacg
     2581 ttctttaaga acccagaata ctaatgcgat ccagagcaga tacgagacat gtggcttaat
     2641 cgaaactaag ctcaggctgg cgccggcaaa aaaccacgct cttctttgca caaaaaatag
     2701 aaatcccaga atgcccagca aaatcaccgg tccgatctgc cccaacagca gcgcgaagta
     2761 gatcggcgga aaaaccagca ctggcaacaa gccatagcgg gcgtttttag gctcgtctgt
     2821 ataggaccgc cacaggatct gtgcaccgac aaaaatgatc aagctgtgga acaggaacca
     2881 aatgaattgc gcggtatcgt aatcgagcat ccccagcggc gagataagcg gcaaagtcca
     2941 aggtgggttc cacataacga gcggctccga ctgcggccac ccaaccgacc tctccatatt
     3001 gagcaattct gttggagaat aaggattgcc accggcgagc aatagcttcg cggcggccca
     3061 atattcaaca aagtcattcg tcacagcgtt tcgatgtgtt acttttctcg atcttgtgaa
     3121 cggagccaat cagggtagca cacggataaa cggctgtgct agtaaattgc cgaggggaga
     3181 agtcgcaatc gcaaaatgga aagtcagtca aaacccaatt ttcttttgat catcaccgat
     3241 cagcaccgcg ccgatcatct cggctgttac gggaacccga tcgtccgaac aaccaacatc
     3301 gatcggctgg caagtactgg cacccgattt gatcgctttt acgtggcgac gccgatctgc
     3361 atgcccaacc gcgcgacctt gatgaccggc agaatgccgt cgttgcatgg cgctcgtcag
     3421 aatggcattc cgttatctct cacggcgaca actttcgtcg agattttgcg ggctgccggt
     3481 tatgatactg ccctgatcgg caagtgtcat ttgcaaagca tcagcggcag tttgccgaca
     3541 atcggaatgc ccattgtcga tttaaccaag accccgccgc cggcgcattt gcgcgaggcc
     3601 gatcgtagtt ggctgactca cggtcgctac gaccaagaac tgcgctcaac ctggataaac
     3661 aatgcggatt tcgagctagc gcttccctat tacggttttg accatgtgga gcttgcggtc
     3721 ggccacggcg atcaagtcgt cggccattac gaccgctggt tcaagcaacg ctgcgccgac
     3781 gccgactcgc tccgcggtgc tcgaaatcaa ttaccgggaa acaactatat tgcgccgcaa
     3841 gcatggcgca cgaaagtgcc cgaggacctg tatccaaccg cgtacgttgc cgaacgcacg
     3901 atgatgtatt tggaaaatta tgcgcgcacg gatcgaagtc ggcccttttt cctgcaatgc
     3961 tcatttcccg acccgcatca tccatttacc cctccggggc gctattggga catgtacgat
     4021 ccgacagccg tcacattgcc acgatcgttt aactccgcgg agcggccgat tccgccgcat
     4081 ttgcaggctc tttacgagga acgaagccaa agggccgcga acaaagacgg ccaacgtaca
     4141 tttgccatca ccgagcgtga ggcgcgcgag gcaattgcgc ttacctacgg catgatctcg
     4201 atgatcgatg atgccatcgg tagtattttg gggtggctaa aaatgcttcg gctcgatgaa
     4261 gatactgtaa ttatcttcac gagcgatcat ggcgatttca tgggcgatca tcagttgtta
     4321 ctcaaaggcg ctctgcatta ccgcggttta gtacgcgtgc cctttatttg gagtgatccg
     4381 gagtcggagt caaagggcta tgtgaacacg ggtctttgcg gcaccctcga tattgcgagg
     4441 acagtcttga gccgcgccgg gcttcaagga cataacggca tgcagggatt gagcctgtta
     4501 cccgcagttc acggcgaaac taccggccat gatgcgctga tcatcgagga acaccagcgg
     4561 cggggctata tgggactgca gaataatttt cgcgcgcgca gtctcattac cggggatctc
     4621 cgtttgactc tgtacgaagg cgtcgaatgg ggcgagctct atgatttggg caaggatccc
     4681 gacgagttga ataacctctg ggacaacccg caatggcgcg atcgtcgata ccagctcacc
     4741 gaaaggttag cgcgaaaaat gatggaactc gccgattcga gccccctcgc cacacatcac
     4801 gggccctaaa gcgagtgctg agctttgagt g
//
LOCUS       DASULZ010000345         3909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_449605, whole genome shotgun sequence.
ACCESSION   DASULZ010000345 DASULZ010000000
VERSION     DASULZ010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449605"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2656
                     /locus_tag="VFY96_07140"
     CDS             <1..2656
                     /locus_tag="VFY96_07140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5606271.1"
                     /translation="MPTLLTPNLSPLARYGFALVVVVVATLLREILSPLLGQGVPFIL
                     FYPAVAVAAWLGGFWPGILSTALSGFLSWYVFMPPYLSWTVLNTSAAGQITIFILASL
                     FICLLAESLHEATRRATQGETKEREQREQYRVTLASIGDAVIATDAKGRVAFMNSVAE
                     ALTGWTYRDASGKPLDEVFKIINEQTRQRVNNPALQALEKGRVVGLTNHSLLIAKDGT
                     ERAIDDSAAPIQTAETGITGAVLVFRDITERRAAERQLIESRERLRITLESIGDGVIA
                     TDAEGRVSFANPVAQRLLGYPAEQTVGRPLRDVFNIVNEFTREPVENPVERVLRDRQV
                     VGLSNHTILIRPDGIEIPVDDSAAPIQDHEGTLAGVVLVFRDISESRRAEKSHATLAA
                     IVQSSDDPIISKDLNGRIMTWNFAAERLFGYREDEVIGQSITLIIPPDRLDEEVEILR
                     RIRQGERLEHYETVRTRKDGTAVEISLTVSPVKAADGSIIGASKIVRDITERRRLERQ
                     LREADRQKDHFIAILAHELRNPISPIQNTIKVLQLERSDDRDLLHYCDLIEKETMHIN
                     RLLGDLLDSSRITQGKLSLQKERLDLSSAVKRAVEASRPAIDEAGLKLAVDLLSQPLI
                     VEADPMRLAQVFSNLLNNASKFTESGGAIRITTEHQGNRAVVRVKDSGIGIAPELMPK
                     IFEMFVQGETVMERRHGGLGLGLTLARDIVEFHGGTIEVKSDGLGHGSEFAVSLPLAA
                     LPRPIESGRAAAIKMAAASVPRRIVIVDDSKNQVLSLERLFKRMGHDVRVAYDAAGAV
                     QVMEEFLPDFALIDIGLPGVNGYDLARRLREQAQFRNVTLIAQTGWGREEDRNQARAA
                     GFDHHLVKPIDHQRLAEILANPASHRES"
     gene            2758..>3909
                     /locus_tag="VFY96_07145"
     CDS             2758..>3909
                     /locus_tag="VFY96_07145"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5606272.1"
                     /translation="MAKPAQKTETIKKSAKRWQSDVIVDMIKRYDFEYIALNPGASYR
                     GLHDSLVNYGENDPPMLLCQHEKIAVQIAHGYARAKGKPMIAIVHDLVGLLHAPMGIY
                     YAYLDRAPVFIIGATGPMAEPKRRPFIDWIHTANGQGEAIRNFVKWDYQPTTVDGVPG
                     AFARAYSVMMSARQGPVYMVYDAGLQEAELTQEVEMPPPGAIDVPSAPSADPVALEKA
                     ASTLAAANRIAIIADFAARPPHGWNHVVELAETLGASVWDVGSRLNFPTDHPLNLTMD
                     PDGCYQDVDVVLTLDIADFEKPTHVRDIATRTVVSKVPANATWIDIGFTDIEISKWSM
                     DYARPFYAQQRMTADPVTTCPQLTRLLKERIAKTSGLGDKIAKRKEEVGK"
BASE COUNT          888 a         1124 c         1100 g          797 t
ORIGIN      
        1 tatgcccaca ctgctgactc ccaatctttc accgctggcg cgctacggct tcgcactcgt
       61 agtggtcgtc gtcgcgacgc ttctgcgcga gatcctgtct ccgctcctgg gacaaggcgt
      121 ccctttcatc cttttctacc ccgccgtcgc cgtcgcggca tggctgggcg gcttttggcc
      181 cggcattcta tcgacagcgc tcagcggctt tctttcctgg tatgttttta tgccgcccta
      241 cctctcgtgg acggttttaa acaccagcgc cgccggtcaa atcacaattt tcatcttagc
      301 cagtctgttc atctgtctgc tcgctgaaag cctccatgaa gccacacgac gcgcgactca
      361 aggcgaaacg aaggaacgag agcaacgcga gcaatatcgc gtcacacttg caagcattgg
      421 cgacgcggtg atcgcaaccg atgccaaggg ccgtgtcgcg ttcatgaatt cggtcgccga
      481 agcactgact ggctggactt atcgggacgc ctcaggcaag ccactggacg aagtattcaa
      541 gattatcaac gaacagactc gccagcgggt caacaatccg gcgttgcaag ctttggagaa
      601 gggccgcgtc gtaggtctca cgaatcatag ccttctcatc gcaaaggatg gcaccgaacg
      661 agcgatagac gatagcgccg caccgatcca gaccgcggaa accggcatta ccggcgctgt
      721 gctggtgttt cgagacatca ccgagcgtag ggcggcggag cgtcaactca tagaaagccg
      781 cgagcggctg cgcattactc tcgaaagcat cggcgatggg gttattgcta ccgatgctga
      841 aggacgggta tctttcgcaa atcccgtcgc gcaaagattg ctgggttatc ccgcagagca
      901 aaccgtcggc cgtccgctgc gcgatgtttt taacatcgtt aatgagttca ctcgtgagcc
      961 tgtcgagaac ccggttgaac gagttctgcg cgataggcag gtcgtcggct tgtccaatca
     1021 taccatcttg attcgccccg acggcataga aatcccggtc gacgatagcg ccgcgccgat
     1081 tcaggatcac gagggaacgc tcgctggagt cgtcctggtg ttccgcgata tcagcgagtc
     1141 cagacgcgct gaaaagagcc atgcgaccct ggctgccatc gttcagtctt ccgacgatcc
     1201 catcatcagc aaagatttga atggccggat catgacctgg aatttcgccg ccgagcgctt
     1261 atttggctac cgtgaggatg aagtcatcgg acagtccatt acgctgatca ttccacccga
     1321 tcgcctcgac gaagaagtcg agatattacg gcgcattcgg caaggcgagc gcctggaaca
     1381 ttatgaaacg gtgcgcacgc gcaaggatgg cactgcggta gaaatttcat tgaccgtctc
     1441 gccggtcaaa gctgcggacg gcagcatcat cggcgcctcg aagatcgtcc gcgatattac
     1501 cgagcgacgc aggttggaac ggcagcttcg tgaggccgat cgccagaaag atcattttat
     1561 cgcgatcctg gcgcacgaac tgagaaaccc aatcagcccg atccagaaca cgataaaagt
     1621 tctacagctc gaacgctcgg atgatcgcga cttgcttcac tattgcgatc tcatcgagaa
     1681 agaaaccatg cacatcaacc gcttgcttgg tgatctcctc gatagcagcc gaattaccca
     1741 aggtaagctc tccttgcaga aagagcggct cgatttaagc agcgcggtga agcgagcggt
     1801 agaagcgagc cggcccgcca ttgacgaagc cggacttaag ttggcggtcg atttgctttc
     1861 ccaaccgctg attgtggaag ccgatccgat gcggctcgcg caggtattct cgaacctact
     1921 gaacaacgcg tcaaagttca ccgaatccgg cggcgctatc cggatcacaa cggagcatca
     1981 gggcaatcga gccgttgttc gcgtcaaaga ttccgggatc ggcattgctc ccgagctgat
     2041 gccgaagata tttgagatgt ttgttcaagg cgaaactgta atggaaagac gccacggcgg
     2101 cctgggtttg ggacttacgc ttgcgcgcga cattgtcgag tttcacggcg gaaccataga
     2161 ggttaagagc gacgggctgg gtcacggtag cgaattcgca gtctcccttc ctctcgccgc
     2221 acttccccgg ccaatcgaaa gcggtcgagc cgctgcgata aagatggcgg ctgcgtccgt
     2281 gcctcggcgc atcgtgatcg tggacgacag taaaaaccag gtgctcagtc tcgaacgttt
     2341 gttcaaaaga atgggccacg acgtgcgcgt tgcctatgat gccgccggcg ctgttcaagt
     2401 catggaggag ttccttccgg attttgcctt gatcgatatc ggtcttcccg gcgtcaacgg
     2461 ttatgacctc gcgcggcggc tgcgcgaaca ggcacagttt cgcaatgtca cactgattgc
     2521 gcagaccggc tggggcagag aggaagaccg caatcaagcg cgcgctgcgg gcttcgatca
     2581 tcatctggtc aaaccgatcg atcaccagcg gttggcggaa atcctcgcga acccggcatc
     2641 ccaccgagaa tcgtagtatc ctgcaatgca gcttccgtaa cggccagtca cgtagcttga
     2701 cgacgacaaa aagatacgga aagaatagga ccgattaaca aacgacggag gaaatgcatg
     2761 gccaagccag cccaaaaaac agagactata aaaaaatcag cgaaacgatg gcagtcagac
     2821 gttatcgtcg acatgatcaa gcgctatgac ttcgaataca tcgcgctcaa tcccggcgcg
     2881 agttatcgcg gtctgcatga ttccttggtc aactacggcg aaaacgaccc gccgatgctg
     2941 ctttgccagc atgaaaagat cgccgtgcaa atcgcccacg gttatgcccg cgcgaagggc
     3001 aagcccatga tcgccatcgt gcatgatctc gtcggcttgc tgcacgctcc catgggcatt
     3061 tattacgcct atctcgatcg cgcgccggtt ttcattatcg gcgccaccgg cccaatggcg
     3121 gagcccaagc gccggccgtt catcgattgg attcataccg ccaacggcca gggcgaagcg
     3181 attcgcaatt tcgtcaagtg ggattaccaa ccgaccactg tagacggcgt gccgggagca
     3241 ttcgctcgag cgtattcggt catgatgagc gcccgacaag ggccggtgta tatggtctac
     3301 gatgcgggat tgcaggaagc ggagttgacg caggaggtgg aaatgccacc gcccggcgcc
     3361 atcgatgttc cgagcgcgcc gtccgccgac ccggtggctt tggaaaaagc ggcgagcacg
     3421 ttggcggcag ccaatcggat tgcgatcatc gccgatttcg ctgcgcgtcc gccgcacggt
     3481 tggaatcatg ttgttgagct cgccgagacg cttggcgcgt cagtctggga tgtgggctca
     3541 cgcctcaatt ttcccaccga tcatccgctg aatctcacca tggatcccga cggctgctat
     3601 caagacgtcg acgtggtgct gaccctcgac atcgctgatt tcgaaaagcc cacgcacgtg
     3661 cgggatatcg ccacgcgtac tgtggtcagt aaagtcccgg ccaatgcgac ctggatcgat
     3721 attggattca ccgatattga gatcagcaaa tggtcgatgg attacgcgcg gccgttctat
     3781 gcgcagcagc gcatgaccgc ggatccggtc accacctgcc cgcagttgac cagacttctt
     3841 aaagaacgaa ttgcgaaaac ctcgggactc ggcgataaaa ttgccaaacg taaagaagaa
     3901 gtcggcaag
//
LOCUS       DASULZ010000346         3992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_449849, whole genome shotgun sequence.
ACCESSION   DASULZ010000346 DASULZ010000000
VERSION     DASULZ010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449849"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VFY96_07150"
     CDS             complement(<1..625)
                     /locus_tag="VFY96_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX5606273.1"
                     /translation="MSIQNSVSLRTDRYFSAGIFGFFLRFKDGGEGGIHMTGYAWESF
                     ITRLLDVGVNAAQGLIRIALLIVLAYVFTRLLRAGLNRLEILLIRATEKTETIPGAAG
                     KRIKTLTGVLWTIGCGLLWFVVVLVAFSQIGFNIGPILAGAGVAGLAVGFGAQHLVRD
                     LVSGFFLLLENQIRVGDTAIVNGTGGLVEAITFRTVVLRDQAGLVHVF"
     gene            676..1683
                     /locus_tag="VFY96_07155"
     CDS             676..1683
                     /locus_tag="VFY96_07155"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606274.1"
                     /translation="MRQTVSWICLLLLLMLCGIESVGAGSAPDKIKFPYSPISWNSLP
                     WWMAKDAGYFEKNGLEVELFYEGASSAIVQAMLAGEANFAGLAGPSIVSNVIGGGDVI
                     QIAAVVRTFTIPMYSQPNINEVSQLKGQKVAVSRFGSISHIAALNVFQKAGVTGVTLI
                     QSGGTPESAAALMSGAVAAAMVPPPQSLMLREKGFRELVGVKQFREWNIPVVENGVAA
                     RRSFVDKNPSVAKRFIRAAFEGIRTIYQNKELTLKVLAKYTKTNDEKILDESYRFSVD
                     ALSKEGFMPPEAFTVLVEQMVSQKSVDEAAAKKLPITAYFDNRFVNELEKEGFFKKLW
                     Q"
     gene            1882..3992
                     /locus_tag="VFY96_07160"
                     /pseudo
     CDS             1882..3992
                     /locus_tag="VFY96_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474795.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
BASE COUNT          966 a         1081 c         1140 g          805 t
ORIGIN      
        1 gaaagacgtg tacgagaccg gcttgatcgc gcaaaactac cgtacggaag gtgatcgcct
       61 ccaccaaacc gccagtgcca ttgacgatgg ccgtatcgcc gacgcggatt tgattttcca
      121 gcagcaggaa aaagccgctc accaaatcac gcaccaaatg ctgggcgccg aaaccgaccg
      181 ccaggcccgc cacgcctgcg ccggcgagaa tggggccgat attaaagccg atttggctga
      241 atgccaccag gacgacgaca aaccacaaca gaccgcagcc gatggtccag agcactccgg
      301 tgagtgtctt gatgcgtttg cccgctgctc cgggtatggt ttcggttttt tctgtcgcac
      361 ggatcaaaag aatttccaga cggttcaagc ctgcgcgcaa tagtctcgtg aacacatagg
      421 cgagcacaat cagcagcgcg atccggatca gtccctgcgc ggcgttgacg ccgacatcca
      481 aaagcctcgt gatgaatgat tcccaagcgt agccggtcat gtgaatgcct ccttcccctc
      541 cgtccttgaa tcgcaaaaaa aacccgaaga tgccagcgga gaaataacga tccgttcgca
      601 gtgagaccga attttgtatt gacatggggc tgtcctggga tagattccgt gccatcgaag
      661 cgtacgaagg aagccatgcg gcaaactgtt tcctggatct gcctacttct tttattgatg
      721 ctgtgcggaa ttgaatcggt tggagcggga tcggcgccgg acaagatcaa atttccgtat
      781 tcgcccattt cctggaatag cctgccatgg tggatggcca aggacgcggg ctattttgaa
      841 aaaaacggcc tcgaggttga gttgttctat gaaggcgcat cgtcggcgat tgtacaagcg
      901 atgctcgcag gcgaggcgaa cttcgctggt ctcgccggtc cttcgatcgt gagcaacgta
      961 atcggaggcg gcgatgtgat tcaaatcgcc gcggtggtca ggaccttcac gattcccatg
     1021 tactcccagc ccaatatcaa cgaggtgtca cagttgaagg ggcagaaggt agccgtgagc
     1081 cgatttggat cgatctcgca tatcgccgcg ctaaatgtct ttcaaaaggc gggggtcacg
     1141 ggcgtgacct taatacagtc gggtggcaca ccggaatccg ccgccgcgct catgtccggc
     1201 gcggtggccg cggcgatggt gccgccgccg cagagcctga tgctccgcga gaagggtttt
     1261 cgcgagctcg tcggcgtcaa gcaatttcgc gagtggaata ttcccgtggt ggaaaacggc
     1321 gtggcggcgc gtcgctcttt cgtggataag aatcccagtg tggccaagcg atttattcgc
     1381 gccgcgttcg aaggcattcg caccatctat cagaacaaag agctgacgct gaaagtactg
     1441 gccaagtata ctaaaacgaa cgacgagaaa attctggacg aaagttaccg tttctcggtg
     1501 gacgccttga gtaaggaagg atttatgccg ccggaagcct tcacagtatt ggtggaacaa
     1561 atggtcagcc aaaaatccgt cgacgaagcc gcggcgaaaa agttgccgat caccgcgtac
     1621 ttcgacaatc gtttcgtaaa tgaattggag aaggagggct ttttcaaaaa gctctggcag
     1681 taacaggctt cgccacgaca tatctcaaat tgcagattgg cgatacactc cggatctgga
     1741 tcgcccattt gtaatacgac ttgagatgat ctgagctctg aaatctgaga tttgaaattt
     1801 ggtcacggcg cagccgcttg gcaagcgacc atgcgatcgc ctaaaatccg cgacatcaat
     1861 gactcgggat aactcctgtt cgtgatcaac gaagccgaca cctgccgaaa atacgttctg
     1921 ccgaagctgt acgccgcggg ctggaacgac gatcagatca gcgagcaaaa gagcttcact
     1981 gacggacgca ttgttcctgt cggtccgaga ggacggcgca agccttgcaa acgggccgac
     2041 tatcttttgg cgctacgcca gggatttcat gcttgcggtc gtggagtcca aggccgccta
     2101 caggcttccc ggcgatggct tgcaacaagc gaaagagtac gcagcgattc taggtcttaa
     2161 attcgcgtac tcgaccaacg gccatggcat tatcgagttc gacttcacca cgggtgcgga
     2221 gcgtgagctc gatgcatttc caaatcccgc cgagctgtgg gagcgttttc gcgcgagcga
     2281 aaaaatcgaa gatgaggaga ccgcaaaccg gcttctgacg ccagccaatg cggcgatcgg
     2341 caagggccca gcgttactac caggaaatcg ccatcaatcg tgtcatccag gcggtgctgc
     2401 agggaaaaaa gagaatcttg ctcaccatgg ccaccggcac aggaaaaacc gctgtggcgt
     2461 tccagatctg ctggaagctt tggaacatac gctggaacag aagcggcgag catcggcgtc
     2521 cgaaaattct ttatctggcg gaccggaata ttctgattga cgatcccaaa gacaagatgt
     2581 tcacgacttt cggcgatgcg cgctggaaga tcgagaacgg cgaagtcaac aaagggcgcg
     2641 aactctattt cgcgatctat caggccatcg ccaaagacga aaaccggcca ggtctttaca
     2701 aagaatactc gaaggacttt ttcgatctga ttatcgtcga cgagtgccat cgcggcagcg
     2761 ccaaggacga cagcaactgg cgcgaaatcc tgcgctattt cgagccggct tttcaattgg
     2821 gcatgacggc gacgccgttg cgtaaagaca accgtgacac ttatcgctac ttcggcaacc
     2881 cggtttatac ctacagcttg cgccaaggca tcgaagacgg atttctcgcg ccctatcggg
     2941 ttcatcgtat cgtgaccgaa tgggacgcgg cgggctggcg gcctagccaa ggcgatctgg
     3001 atcggtaccg ccggacgata ccggacaatg aatatcaaac ggtcgacttc gagcgcgccg
     3061 tcgcgctcaa ggcgcgcacc caggcgatcg cgcgcaatct tacggatttc atgaagaaga
     3121 ccgatcgctt tgccaagaca atcgtattct gcgtcgatca agagcacgcg gatgagatgc
     3181 gcgccgcact caataatctg aacgctgatc tggtcaaaca gtatccggac tatgtttgcc
     3241 gcatcacttc ggacgagggc agcctcggaa agggcctgct cagcaagttt caggaactcg
     3301 agaccgggac gccggtgctc gtgaccacat cgcaattgct tacaaccggt gtcgatattc
     3361 ctagttgtaa gaatatcgtc ctcgctcgcg tggtgggctc tatggtggaa tttcaagcag
     3421 atcatcggcc gcggcacacg ggtgcgcgac gactatgaca aatattattt caacattctc
     3481 gactacacgg gttccgcgac gcgactgttc gccgaccggg actttgacgg cgatcccgcg
     3541 ctggtcacgg agcaggagat gaccgaaacg ggtgaaacaa tcagtgaact ggtgacagaa
     3601 gaaacaaccg tcgcggaatc ggaagaagac agcgccgcga caattaccga cgactcggaa
     3661 ggcgtgcggc gaaaatatta cttcgacggc ggcaacgtcg agatcgccgc gcatttggtt
     3721 tatgagatcg atccggaagg ccggcaactg cgggtggtga agttctccga atacgcggcc
     3781 gagaaagtgc gcacgcttta tccttcggcc gctgacttgc ggaagcagtg ggccgatccg
     3841 gtacttcgag cggagattat tgccaaactc gaagaacgag gcatcgactt cgatcagctg
     3901 cgagccacca gcaatcagcc ggaggccgat ccgttcgacc tgctttgcca tctcgctttc
     3961 aacgggccgt tgcgcacgcg gcgcgagcgt gc
//
LOCUS       DASULZ010000347         5810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_44485, whole genome shotgun sequence.
ACCESSION   DASULZ010000347 DASULZ010000000
VERSION     DASULZ010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44485"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFY96_07165"
     CDS             <1..273
                     /locus_tag="VFY96_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606275.1"
                     /translation="KLRWEDGSEGPLRLLESIEAVALGIDGKLALWRALETVAEVAAE
                     MRKVDYGRLSARAQEQREVAESMRLMAARLALVPETESPRPAASSH"
     gene            complement(270..656)
                     /locus_tag="VFY96_07170"
     CDS             complement(270..656)
                     /locus_tag="VFY96_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HEX5606276.1"
                     /translation="MKNHHSWFPTLARWASTAAGSPITFAVALLIVLTWAVTGPFFNY
                     SDTWQLVINTGTTIVTFLMVFLIQSTQNRDTQAVQLKLDELIRVTKGAHNLLIDLEEL
                     DDEQLEQLRRTYERLGKMAREKPKSN"
     gene            complement(1079..1333)
                     /locus_tag="VFY96_07175"
     CDS             complement(1079..1333)
                     /locus_tag="VFY96_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX5606277.1"
                     /translation="MGVLGWILFGLIVGVVGKLLMPGRDPGGFILTILLGIAGALLGG
                     FIGQSLGLYQPGEPAGFIMAVVGSIILLLIYRFVIGRRSY"
     gene            1521..4058
                     /gene="ligD"
                     /locus_tag="VFY96_07180"
     CDS             1521..4058
                     /gene="ligD"
                     /locus_tag="VFY96_07180"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX5606278.1"
                     /translation="MKSSKKFGGKELAISNPDKVMFPEKRFTKSRVIEFYAAIARYLL
                     PYIKDRPITMKRFPNGVGGEHFYEKDAPSFTPSWIKKFPVPRTGENSMIHYILLNDLP
                     SLVWSANMANLEIHPFLAKIPHIERPTMLVFDLDPGEGADILSACKAAFHVKDVLDRL
                     NLKSFVKVSGSKGIHLHVPLNTNVTYEVTQLFAKSIAQLLEREHPNLVVSEMPKTKRR
                     GKVFVDWSQNAEYKSTVAVYSLRAKAKRPFVAMPVSWNELTEAIKKGDAAQLFIEPDL
                     ALRRLKKTGDLFAPVLKLKQTLPEPFLELMRKESEDHQPHRTKALDAYRRKRDFTKTP
                     EPGPALPKSSRQGSRKLFVIQKHAARRLHYDFRLEMSGTLKSWAVPKGPPYELNERRL
                     AVAVEDHPMDYAKFEGIIPRGEYGGGTVMVWDIGTYELIDGNYWQGKLHIFLSGEKLK
                     GEWVFVKDAGRDGKDNVWYCIKAGASVAPPRANEEDSSALSGRSMDEIAGAADAVWHS
                     NRNGSDTIVQADSVSEETMDALPKAKVKFVEPMLAKSVTELPEDRRAWIYEVKVDGYR
                     CLVGKDRSAVKLWSRRGNLFNQDFPGLARACAALPPDTLVDGEVVALDQQGRMSFNLL
                     QHRRSQASAIRFYAFDLLVYKGHSAINLQISERHDLLAQALANVGGDVQLMQRFETGS
                     AELIRAAKRLGFEGIIAKRRDSLYEPGKRSGAWLKYKINQEQEFVIGGYTPGHPFDAV
                     IVGYYRDGQLIYVAKVRNGFVPRIRREVAKRLRAVETDVCPFANLPEKKRTAWALTKE
                     EMNKCVWIRPELVAQIEFTEWTPDGHLRHATFAGLREDKTPQEVVRE"
     gene            complement(4285..4644)
                     /locus_tag="VFY96_07185"
     CDS             complement(4285..4644)
                     /locus_tag="VFY96_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606279.1"
                     /translation="MADTSETRIGDSSEAQQFFDTFRRSEYLEPETALIAAILEDAIH
                     DYRKYRSARDAAGKERFREAEEWLMHEGNGWIFSFGNVCEFLGLDPEYVRRGLRETEG
                     SGSDLEKNRRNASKRAA"
     gene            complement(4932..5363)
                     /locus_tag="VFY96_07190"
     CDS             complement(4932..5363)
                     /locus_tag="VFY96_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606280.1"
                     /translation="MKTASARNLLSFFAIAYLTLGGFRPTAWAQTIRDREATARIVSV
                     EKLSVTDASVSGEVINQSKNTLRDVQVLIRYIWLWDNETKPGNNDPSASFYYNLPGEV
                     PPGGRASFTFKPSQPLAKASGGHFETSASIAGFSEVFLPSR"
     gene            complement(5568..>5810)
                     /locus_tag="VFY96_07195"
     CDS             complement(5568..>5810)
                     /locus_tag="VFY96_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606281.1"
                     /translation="FEGVNNTLKLIGDQYLARFYRVVNRRFHLDEWDASILRKLQTLE
                     SIYEKISVQASNRRLELLEWVIIFLIAFSIVLELIH"
BASE COUNT         1383 a         1543 c         1561 g         1323 t
ORIGIN      
        1 aagttgcgtt gggaagacgg tagcgaaggg ccactgcgat tattggaaag tatcgaggct
       61 gtggctctgg gaattgacgg taagctggcg ctgtggcgcg cccttgagac ggttgcggaa
      121 gtcgctgccg agatgagaaa agtcgattat gggaggctga gtgcgcgtgc tcaggagcag
      181 cgcgaagttg ccgagtcgat gcgtctgatg gcagcccgat tggctttagt tccagagaca
      241 gaatctcctc gaccagccgc ttccagccac tagttggatt tgggtttttc cctggccatt
      301 tttcctaatc gttcataggt ccgccgcagc tgctcgagct gttcgtcgtc gagttcctcc
      361 aaatcgatca gcaagttatg cgcacctttg gtgacgcgga tcagctcgtc caatttgagc
      421 tgcaccgcct gggtgtcccg gttttgcgtg ctctggatca ggaacaccat caaaaatgtg
      481 acaatggtcg tgcctgtgtt gatcacgagc tgccatgtgt cgctatagtt aaagaaagga
      541 cccgtcaccg cccatgtgag gacaatcagc agggcgacag caaaggttat cggactgcca
      601 gccgcagtcg aggcccatct cgccagtgtc ggaaaccacg agtgatggtt tttcatcgaa
      661 gttcctttct gcgtagacga cgcaaattca cgtcaaagca cattgcgctc cgctaggttc
      721 gattcactta ggtcgatttg attcgtctga ggaatggacg gactcagcgg cttactcagc
      781 attgctcggt ggtgcagcct gtgctctcgc aaataagtgg tgattgttcg ggggatctgc
      841 taccctagcc tcctcatgtt tgtctcaggt ctaatattcc tcagtttttt ctggcacctg
      901 gtacgcagcg tgcccggcct gttcgaatcc ctcggctatc tttttgggat tctgttgggc
      961 tccttcactc ttgacgcttg ccttgacgac gacggggagt catagcccgc cttggcttct
     1021 tcgcccggag cgaaatggga gcgctcggca ccaggagcac cgagcgccag catccccgct
     1081 aataactgcg cctgcctatc acgaagcgat aaattaacag aagaataatt gacccgacca
     1141 ccgccatgat aaagcccgca ggttcacccg gctgataaag cccgagggat tgtccgataa
     1201 agccacccag caatgcccca gcgataccaa gaaggattgt cagtataaat cctccgggat
     1261 cacggccagg catcagcagt tttccaacaa ctcctacgat caagccgaac aggatccagc
     1321 ctaaaacgcc catattctaa acctcctcac tttaaatttt ttctcccccg tcattcggcg
     1381 attgggtgag caacgcttat gccaccgccg tcaccgggtt atcagcgaat gcactgctga
     1441 acgtgcagcc ctcgatatgc ttggatgctt atgtgtcgag ctagcgcatc gatctgtgaa
     1501 attgtagaaa tctaacaatc gtgaagagct caaaaaaatt cggcgggaaa gaactggcaa
     1561 tctctaaccc cgacaaagtg atgtttcccg aaaagcgctt taccaaatcc cgggtgatcg
     1621 agttctacgc tgctatagcg cggtaccttt tgccgtatat caaagatcgg ccgatcacga
     1681 tgaagcggtt tcccaatgga gtcggcggcg agcattttta tgagaaggac gcgccctcct
     1741 tcacgccgtc atggatcaaa aaatttcccg taccacgaac gggcgagaac tcgatgatcc
     1801 attacatcct gctcaatgac cttcccagcc tcgtatggtc agccaatatg gccaatctcg
     1861 aaatacatcc tttcttagcc aagattccgc acatcgagcg gcccaccatg ttggtcttcg
     1921 atctcgaccc cggggaaggt gccgatattc ttagtgcctg caaagcagcc ttccatgtga
     1981 aggacgtgct cgatcggctc aatctgaagt ctttcgtaaa agtctccggc tcaaagggca
     2041 tccacctcca cgtgccgctc aacacgaacg tgacgtacga agttactcag ttgtttgcca
     2101 aaagcatcgc tcaactactc gaacgcgagc atccgaatct cgtcgtttcg gaaatgccga
     2161 aaaccaagcg aagaggcaag gtgttcgtcg attggagtca aaacgccgag tacaaatcga
     2221 cggttgcggt ttattctttg agagccaagg cgaaacggcc atttgtggcg atgccggtga
     2281 gttggaacga attgactgaa gcgataaaaa aaggtgacgc ggcgcagctc tttatcgagc
     2341 cggatcttgc gctacggcgg ctcaagaaaa ccggagatct ctttgcgccg gtgctgaagc
     2401 tgaagcagac actgccggag ccctttctcg agctgatgag gaaagaatca gaagatcatc
     2461 agccgcaccg taccaaggcg cttgacgcct atcggcgcaa gcgcgatttc acgaagactc
     2521 ccgaacctgg tcccgcgctg ccaaaatcaa gccggcaagg aagccgcaag ctctttgtca
     2581 ttcaaaagca tgccgcccgc cgtttgcact acgactttcg cctggaaatg agcggtacac
     2641 tcaaatcatg ggcggtgccc aaggggccgc cctacgagct gaacgagcgg cggcttgccg
     2701 tcgcggttga agaccatccg atggactatg ctaaatttga aggcatcatc ccgcgcggag
     2761 aatacggcgg cggcaccgtg atggtctggg acattggaac ttacgagttg atcgacggca
     2821 attattggca gggcaagctg cacatttttc tgagcgggga aaagctcaag ggcgaatggg
     2881 tattcgtcaa agacgccggt cgcgatggga aagacaacgt ctggtattgc atcaaagccg
     2941 gtgcctcggt tgcgccgccg agagccaatg aagaagattc ctcggcgctc agcggccgga
     3001 gcatggatga gatcgcgggc gccgcggacg cggtttggca cagcaatcga aatggttccg
     3061 acacgattgt gcaggctgac tccgtgtcgg aggagactat ggatgcgctg cccaaggcca
     3121 aagtgaagtt tgtcgaaccg atgcttgcga agtcagttac ggaactgcca gaggatcggc
     3181 gcgcctggat atacgaggtg aaagtcgatg gctatcgttg tctcgttgga aaagatcgca
     3241 gcgccgttaa gctttggtct cgccgcggca atttgttcaa tcaggacttt cccggcctag
     3301 cgcgagcctg tgccgcgctt ccccctgaca cgcttgtgga tggagaagtc gttgctttgg
     3361 atcagcaagg ccgaatgtcc tttaacttgc ttcagcatcg tcgctcgcaa gcgagcgcaa
     3421 ttcgctttta tgcgttcgat ctactggttt acaaaggtca tagcgccatc aatctacaga
     3481 tctcagagcg gcacgatctc ctcgcgcaag ctcttgcgaa tgtcggcggg gacgttcaat
     3541 tgatgcagcg atttgaaacc ggctccgcag agctgatccg ggctgccaag cgactcggat
     3601 ttgaaggaat aatcgccaaa cgcagagatt cgctctatga accggggaaa cgaagcggcg
     3661 cgtggctcaa atacaagatc aaccaggaac aagaatttgt gatcggcggc tatacgccag
     3721 gtcacccgtt cgatgctgtc attgtcggct actatcgaga tgggcaattg atttacgttg
     3781 cgaaggtacg taacgggttt gttccacgaa tacgccgtga agttgccaaa cgactgaggg
     3841 ctgttgagac ggatgtgtgt ccatttgcaa atctgccgga gaaaaaacgc actgcgtggg
     3901 cgctgacaaa ggaagaaatg aataaatgcg tatggattag gcccgagctc gttgcccaga
     3961 tcgagttcac cgaatggacg cccgacgggc atttgagaca cgccactttc gcaggattga
     4021 gggaggacaa aaccccgcag gaagtcgtcc gcgaatgagc cggaccggtc ccgcgcaaat
     4081 cgctttcgcg tcatgtcgga tagagatttt tcgcgcactt aacagctcac acaagcgact
     4141 tcgccgcggt tgaaacgctc ggcgtcgatt cgcggcgccg acgaggtcgc acgcgccgga
     4201 tcattgccaa tataatcggg ccgcagctgc tgttcggatc cttacggtga gaaactttca
     4261 tcttaaaaga aagcttcctg ccactcatgc agcgcgcttg ctagcatttc gtcggttttt
     4321 ttcgagatcg cttccactac cctccgtctc acgtaatccg cggcgcacgt actcggggtc
     4381 gagtcccaga aactcacaca cattgccgaa agaaaaaatc cacccattgc cttcatgcat
     4441 gagccattct tcggcttctc gaaaccgctc tttgcctgcg gcatcccgcg cggagcgata
     4501 tttccggtaa tcatgaatgg catcttcgag aatggcagcg atcagcgctg tttccggttc
     4561 gagatactcg ctgcgccgaa aggtatcgaa aaactgctgc gcctccgaac tatccccgat
     4621 tctcgtttca ctagtgtctg ccatatttcg ccctcctggc ttcttaccta ataaacaacc
     4681 cgcaaccatc atgccatgcg tatattcgtg gctagtaagc ttttacgtct gtaatagatc
     4741 ggtttccttt cgcacatctc ggaaagatct agtgagaact gatcaaaatt aaaaaaagct
     4801 aagggcatct tggtgcgtat tgtgtaaccc ctcgcacctc acagttgtgc ccctctgcga
     4861 ttcgtgacag tgataacgcc gcggattgcg agggtgatcg cgagaaagcg ccaacaaaaa
     4921 attggaagtt tttatcggct gggcaaaaat acttcagaaa atccagcgat agacgctgaa
     4981 gtttcgaagt gccccccaga cgccttcgca agcggttgcg aaggcttgaa ggtgaatgac
     5041 gcccgccctc ctggcggtac ttcacccggc aggttgtagt agaacgacgc gctcgggtca
     5101 ttgtttccgg gtttcgtctc gttgtcccag agccagatgt aacgaatcaa cacctgcaca
     5161 tcgcggagag tattttttga ctggtttatg acttcaccgg aaactgaagc atcagtgacc
     5221 gagagctttt caaccgatac gatgcgcgcg gtggcttccc gatctctaat cgtttgtgcc
     5281 catgcggtgg ggcgaaagcc gcccagggtt aggtatgcaa ttgcgaaaaa agaaagcagg
     5341 ttacgagccg atgcggtctt catggaaaac ctcccgatga gatgcgttaa cgctacgggg
     5401 cggttgcgca aagtgatctt tcggagacga aattatcgta tgccatggtg ccggttagtc
     5461 aatacgggtg atccgttcga gcgacgacct cgacgttacg tttcgagaga aacctgccaa
     5521 cacgagtgcg caagcgttac actgatgcaa gccgggaaag ccattgctca gtggattagt
     5581 tccaagacaa ttgaaaaagc gatcaaaaag ataatcaccc actcgagcag ttccagtcgg
     5641 cgattggacg cctgaacaga gatcttttcg taaatactct ccaacgtttg tagtttgcgc
     5701 agaatgcttg catcccattc gtctaggtga aagcgtcggt tgaccacgcg atagaatcgc
     5761 gccaggtact gatccccgat cagcttgagt gtattgttca cgccttcaaa
//
LOCUS       DASULZ010000348         2866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1057435, whole genome shotgun sequence.
ACCESSION   DASULZ010000348 DASULZ010000000
VERSION     DASULZ010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057435"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VFY96_07200"
     CDS             <1..296
                     /locus_tag="VFY96_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5606282.1"
                     /translation="VKFLHDRRDMAKKITDANVELTKWIQQNQAEAQKLLIDELKAET
                     RADFAQDAVAQAWNRIQFTSDVSRDLIAKSVQDGKDAGFFKGSTDTSKLVETP"
     gene            296..1129
                     /locus_tag="VFY96_07205"
     CDS             296..1129
                     /locus_tag="VFY96_07205"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5606283.1"
                     /translation="MHRVPRHRSSAAEELEIIAPSKLSIEDVSKNFKGASGSVLALDR
                     VSLNVAEGEFVCLVGASGCGKTTLLNIIAGLEKPDSGTVQADGKPITGPGRERLVMFQ
                     EPALFPWLNVFGNVLFGLKLKPNLTNKDRRDVAKYYLELVGLSRFERANIHELSGGMK
                     QRVSLARALAPNPRVLLMDEPFAALDALTREQLYGDLQRIWKARKKTIVFVTHNVREA
                     ACLGDRVIVFSPRPGRIREEFAVDLPRPRDINSVDLAVHSTEITKALKSYSQPEVAIA
                     E"
     gene            1276..1893
                     /locus_tag="VFY96_07210"
     CDS             1276..1893
                     /locus_tag="VFY96_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5606284.1"
                     /translation="MVKAIIVTMRRLVIGYIIGLVVGLPLGLLTARWKVLQDTIGTLA
                     LGLQTLPSVCWVPLALLWFGQTEAAMLFVVIMGTLWSVVIATETGVRNVPPIYSRAAL
                     TMGSKRLHMWFKVILPSALPFIVSGMKQGWAFAWRSLMAAEIFVTILTGFGLGQLLHY
                     GRELNAMEQVIGIMIVIVVIGLLADKIFFSPIEKFLHRRWGTSQA"
     gene            complement(2189..>2866)
                     /locus_tag="VFY96_07215"
     CDS             complement(2189..>2866)
                     /locus_tag="VFY96_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase-like
                     domain-containing protein"
                     /protein_id="HEX5606285.1"
                     /translation="HFTLEIDNRVESPSVNFYGMDCWTFFETALGFARMLNEPESNWT
                     PERLLYYIEMDRYRGGQCTGEYLSRLHYLEDWLYDNNRRGLVEDMTRDLGGRSVPHSA
                     REMTVGWRHYRYLAANKSLLGPLARMEANVSSRALYEIPKSQVAKIEPKLRSGDIIGV
                     ISRERNGLHSTAHVGLALRTSDGVLHFMHASSPSNYGKVVVDDQLSKYLFRYGSNSGI
                     LVGRPMR"
BASE COUNT          654 a          747 c          789 g          676 t
ORIGIN      
        1 gtgtgaagtt cctgcatgat cggcgcgaca tggccaagaa aatcacggat gcgaatgtgg
       61 aactgaccaa atggatacag cagaatcaag ccgaagcgca gaagttattg atcgacgaac
      121 tcaaagcgga aacgcgcgct gacttcgcgc aggatgccgt cgcgcaggcg tggaatcgga
      181 ttcaattcac gagcgacgtc tctcgtgatt tgattgcaaa gtcagtgcag gatgggaaag
      241 atgcgggctt ttttaagggc tcgacagaca cttccaagct ggtcgaaact ccataatgca
      301 ccgagttccg cgccatcgtt cctcagccgc tgaggagctc gagatcatcg cgccctcgaa
      361 actctcgatc gaagacgttt cgaaaaactt caaaggcgca tccggatcag tcctcgcgct
      421 tgatcgtgtg agcttgaacg tcgccgaggg tgaatttgtg tgccttgttg gtgccagcgg
      481 ttgcggcaag acgacattgc ttaacatcat tgccgggctc gaaaaacccg acagcggaac
      541 cgtgcaggcc gatggtaaac ccataaccgg cccggggcgc gagcgtctcg tgatgttcca
      601 ggaaccggcc ttgttcccat ggctgaatgt gtttgggaat gttttatttg ggctcaaact
      661 gaagccgaat ctgacgaaca aagaccggcg cgacgtggcc aagtattacc ttgaacttgt
      721 cggtctcagc cggttcgagc gcgccaacat tcacgagctt tctggtggaa tgaagcagcg
      781 tgtgtcactt gcccgtgcgc tggcgccaaa tccgcgcgta ctgctaatgg acgagccgtt
      841 cgccgctctc gatgcgttga ctcgtgagca actctatgga gaccttcagc ggatctggaa
      901 agcgcgaaag aaaacaattg tgtttgtcac gcataacgtc cgcgaggcag cctgcctcgg
      961 cgaccgcgtc atagtttttt caccgcgtcc aggtcgtatt cgcgaagaat ttgccgtgga
     1021 tttgcctcgg ccgcgggaca tcaacagtgt cgaccttgcg gtccattcaa cggagatcac
     1081 gaaagcgtta aaaagttaca gccaaccaga ggtcgcaatc gcagaatgaa acgttttttg
     1141 ttggcggccc ttttttttgc ggttcttctg ctaatttggg aggttctgtt tcgtgcaaag
     1201 atttggtcgc ctgtccttct cccgtcgcca aaacaagtcg cagtttattt agaaagcgcg
     1261 gctgcggatg gaaccttggt aaaagcaata atcgttacga tgcgccgatt ggtcattggc
     1321 tacatcattg ggctcgtcgt agggctgccg ttggggttgc tgaccgcccg atggaaggtg
     1381 ttgcaagaca caattggaac gctcgccctc ggactccaga cattgccgag cgtctgctgg
     1441 gtaccgctcg cgttattgtg gttcggacag acagaagccg ccatgctttt cgtggtaata
     1501 atgggcaccc tatggtcggt agtgatcgca accgaaactg gagttcgtaa cgtcccgccg
     1561 atttacagcc gtgctgcttt gaccatgggc tccaagcgtc tgcacatgtg gttcaaggtt
     1621 atcctcccct cggcacttcc ttttatcgtt agcggaatga aacaaggttg ggcattcgcg
     1681 tggcgttcac taatggctgc cgagattttc gtaaccattc tgaccggttt cggtctagga
     1741 cagcttctac actatggccg cgaattaaac gccatggaac aggttatcgg tatcatgatt
     1801 gttatcgtgg tcatcggttt actggccgac aaaattttct tttcgccgat tgaaaaattc
     1861 ctacatcggc gttgggggac cagtcaggca tgattttcag gcaaaagcga gtgcccgtgt
     1921 ccgcgcgacg cgagatgcgt gttagcaata agctgggact acacgcacgc cctgccgctc
     1981 actttgtgaa acacgtgagg acgtttcgat ctgaaatctg gcttgtcacc gctgacggcc
     2041 ggtattcggc ttcaagcttg atcgaggttc tccgcgccaa tcttgattgc ggggctaccg
     2101 caacgcttga ggcatatggc gttgatgccg aggaggctgt agaacgacta ggaaaattgc
     2161 tcggcgaatt ggataagttc gacgaatcct agcgcatagg tcggccgacc agaattccgc
     2221 tgttggaccc gtaccgaaag aggtatttcg agagctgatc gtccacgaca actttgccgt
     2281 aattgctagg ggacgacgcg tgcatgaaat gcaaaacgcc gtcgctggtg cgcaatgcca
     2341 gcccgacatg cgcggtcgaa tggagtccgt ttcgctctcg cgaaatcacg ccaataatgt
     2401 cgccgctgcg caatttcggc tcgatcttgg cgacctggct ttttggaatt tcatagagtg
     2461 ctcgcgagga aacattcgct tccatgcgcg caagcggccc gagcaaagac ttattcgcag
     2521 caagataccg atagtgccgc caaccaacgg tcatctcgcg cgcagaatgg gggacgctac
     2581 gtccgcccaa atcgcgtgtc atatcctcta ccaatccgcg ccgattgtta tcgtaaagcc
     2641 agtcttccag gtaatgcagc cgggaaagat attcgcccgt gcattgaccg ccgcgatatc
     2701 gatccatctc gatgtaatac aacaaacgtt ctggagtcca attggattcg ggttcgttta
     2761 acattcgcgc aaacccaagt gccgtttcga aaaacgtcca gcagtccatc ccataaaaat
     2821 tcaccgaagg cgattcaacc cggttatcga tctcgagggt gaaatg
//
LOCUS       DASULZ010000349         2602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_652730, whole genome shotgun sequence.
ACCESSION   DASULZ010000349 DASULZ010000000
VERSION     DASULZ010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652730"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /gene="rplT"
                     /locus_tag="VFY96_07220"
     CDS             <1..302
                     /gene="rplT"
                     /locus_tag="VFY96_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012673446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX5606286.1"
                     /translation="AKGYVGGRARLYRSARETVERALSFAYRDRRVKKRTFRGLWITR
                     ISAAAKMNGISYSQLIKGLKQAGVELDRKVLAEIAAADLPGFNQLAETAKSHLGA"
     gene            325..1365
                     /gene="pheS"
                     /locus_tag="VFY96_07225"
     CDS             325..1365
                     /gene="pheS"
                     /locus_tag="VFY96_07225"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX5606287.1"
                     /translation="MNDSLEALRGEVLERIQQAHSEQELEQIRIDVLGRSGKVTLMLR
                     GLRDVAPEQRPRQGERLNQLRRAVEVSLEGRLETVKEQAKAKALAEERVDITLPGTRP
                     MRGASHPLTLVIDEIIDIFSGMGFEVARGPDIEDDYHNFEALNFPKDHPARDMQDTFF
                     VSNDRVLRTHTSPVQIRTMEARKPPLQVVVPGAVYRHDDDATHSPMFHQVEGFMVDEA
                     IAFSDLKGVLTSFLRQIFEGDFGVRFRPSFFPFTEPSAEIDIQCVICSGAGALSSGQT
                     CRVCKASGWLEILGAGMIDPAVFNFVGYDPEKVSGFAFGMGVERIAMLKYGIDDIRLF
                     FQNDLRFLRQFS"
     gene            1431..>2602
                     /locus_tag="VFY96_07230"
     CDS             1431..>2602
                     /locus_tag="VFY96_07230"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX5606288.1"
                     /translation="MKLTFNWLKEFVAFTGAPEELVQLLTMAGFEVESLSSLREADTN
                     REDWLLEIAVTPNRGDCLGIAGIAREVAALTGAELKPIATGLSEKTPQLEKRVTIRIE
                     DRNLCPRYSARIIDHLSITESPAWLRYRLEACGIRAINQVVDVTNYVMLETSQPLHAF
                     DWDRLPEKSIVVRPACEINQFTTLDGVTRELARDDLLICAGEIPVAIAGVMGGSNTEV
                     RASTSTILLESANFSSSSIRRTAKRLGLHSEASHRFERGVDPEGTIRALDRAVWLLQE
                     ISGGIQMSGVADEYPGRVTPAALGLRAERIEQLLGVGIELEHAETLLRALGLNVEREP
                     NRRRLSVTVPATRPDLTREADLIEELARLNGYDKIPTRLPASRISAKRDGRLAWER"
BASE COUNT          578 a          681 c          789 g          554 t
ORIGIN      
        1 tcgccaaagg gtatgttggc ggtcgggcaa ggctctatag gtcggcccga gaaacggtgg
       61 agcgtgcgct atcgttcgcc tatcgtgatc ggcgggtgaa gaaaagaact tttcgcggat
      121 tatggattac ccggatcagc gcggcggcaa aaatgaatgg catttcatac agccaactga
      181 tcaaggggct caaacaagct ggtgtcgaat tggaccgcaa ggtcctggcg gaaatcgcgg
      241 cggcagacct gccgggattc aaccaattgg ctgaaaccgc aaaatctcac cttggcgctt
      301 aaacgctaga atcttcgcag tcgaatgaac gattccctgg aagctctccg cggagaggtt
      361 ctggaacgta tccagcaagc ccattctgag caagagctgg aacagatccg aatcgatgtc
      421 ctcggccgga gcggtaaagt tacactcatg ttgcgcgggc ttagggatgt cgctcccgag
      481 cagcggccgc ggcagggtga gcggttgaac caactccggc gggccgtcga ggtatcactg
      541 gagggccgac tcgagacggt gaaggagcaa gctaaggcca aggctcttgc cgaggagcgc
      601 gtcgatataa cgcttcccgg gacacggccg atgcgaggag cctcccatcc gctgacactc
      661 gtgatcgacg agattatcga tattttctcg ggcatgggat tcgaagtcgc gcgtggaccg
      721 gacatcgagg atgattacca taatttcgag gcgcttaatt ttcccaagga tcatccggcc
      781 cgcgacatgc aagatacttt ttttgtttcc aatgaccggg tactccgaac tcatacatca
      841 ccggtgcaaa ttcgcacgat ggaagcgcgc aagccgccgc ttcaagtggt cgtccccggt
      901 gcggtttacc gccacgacga tgacgcgaca cattcgccga tgtttcatca ggtagaaggg
      961 ttcatggtgg acgaagcgat tgcgttctct gacttaaaag gggtgctcac cagttttctc
     1021 cggcagatct ttgaaggaga tttcggcgtg aggtttcggc cgagtttttt cccgtttacc
     1081 gaacccagcg cagagatcga catacaatgc gtcatttgca gcggtgccgg agcgctcagc
     1141 tccggtcaga cttgccgggt gtgcaaagcg agcggttggt tagagattct cggcgcgggg
     1201 atgatcgatc cggcggtttt taacttcgtc ggctacgatc ctgaaaaggt ctccggattt
     1261 gcatttggga tgggagtcga aagaatcgcg atgctcaagt acggcatcga tgatatccgg
     1321 ctgtttttcc aaaatgactt gaggttttta aggcagttta gttaactccg cactggacga
     1381 ctgccggcag aggcaccgac acttccgtag gcccaaaccg caatcgtttc atgaagctta
     1441 cttttaattg gctcaaagag tttgtcgctt tcaccggcgc gccagaggag ctggtgcaat
     1501 tgctgacgat ggccggattc gaagtggagt cactgagttc attgcgcgag gctgacacga
     1561 atcgtgaaga ttggctgctc gagatcgcgg tgacgccgaa ccgtggcgat tgcctcggca
     1621 tcgccgggat tgcgcgcgaa gttgccgcgc tgaccggtgc tgagcttaaa cccatagcga
     1681 cggggctgag cgagaaaacg ccgcagctcg agaaacgcgt cacgattcgg atcgaagatc
     1741 gaaatctttg cccgcgctat tcagcacgaa tcatcgacca tctttcgatc actgaatcac
     1801 cggcctggtt gcgctatcgg cttgaggcct gcggcatacg cgccattaac caggtcgtcg
     1861 atgtcaccaa ctatgtcatg cttgaaacga gtcagccgct tcacgctttt gattgggacc
     1921 ggctgccaga gaaaagcatc gttgtccggc ccgcgtgcga gattaatcaa ttcacaacat
     1981 tggatggcgt aacgcgggag ctcgcgcgcg acgatttgct gatctgcgcc ggggaaatcc
     2041 cggtggctat cgccggcgtc atgggcggaa gcaacaccga agtgcgcgct tcgacttcta
     2101 cgatcctgct ggaaagcgca aatttttctt catcgagcat acggcgaacg gcaaagcgcc
     2161 ttgggctcca tagcgaggca tcgcatcgtt ttgagcgcgg cgtcgatcca gagggaacta
     2221 tccgggcact cgatcgcgct gtctggttgc tgcaggaaat ttccggcgga attcaaatga
     2281 gcggggtagc tgatgaatat ccgggtcgcg tcacaccggc agcgctaggg ctacgagccg
     2341 agcggatcga gcaactgctt ggcgtcggta tcgagcttga acatgcggaa acgctgcttc
     2401 gcgcgctcgg gctgaacgtt gagcgagagc caaatcggcg gcgcctaagt gttacagtgc
     2461 ccgcaacccg gcccgatctg acgcgggaag ccgatttaat cgaagagttg gcgcggctaa
     2521 acggctacga caagattccg acgagattgc cggcttctag aatttcggcc aagcgcgatg
     2581 gacggctggc ttgggaacga aa
//
LOCUS       DASULZ010000350         3610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_44566, whole genome shotgun sequence.
ACCESSION   DASULZ010000350 DASULZ010000000
VERSION     DASULZ010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44566"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..882
                     /locus_tag="VFY96_07235"
     CDS             79..882
                     /locus_tag="VFY96_07235"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606289.1"
                     /translation="MAKLVLAAGVPHPPRLVFEMQQAPGKVRGELLMKQVREQVEKAE
                     PDVIIEVDSDHFVNFFYNNLPSFCIGMAEEAQGPQEDWCPMPRYTVHGHVPFAKALYK
                     YGVHDKFDLAAAHELRLDHSLTVPLHFLNPDMRLPVVPIYTNGFASPLPLATRCVALG
                     RMVRRFIEAWEGNERVALIASGCFAMDVGGPLRGWIDTEWAETISQLLVRGQYQTLAR
                     RATEERINAAGNNSGELLNWITVAGAVGKTKPLFLEPDEGNGYAVWKFE"
     gene            1151..1474
                     /locus_tag="VFY96_07240"
     CDS             1151..1474
                     /locus_tag="VFY96_07240"
                     /inference="COORDINATES: protein motif:HMM:NF019366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606290.1"
                     /translation="MSVYQINKLLYLTDNDPAFRKRMQEEPEKVLSEFKLTAEEHAAL
                     TSGAVGKLYQMGVHTFLLNHLYRYELFGVNRDNYLSRIREGMPYDRRFEQGNMPVQRF
                     IKTKE"
     gene            complement(1632..2645)
                     /locus_tag="VFY96_07245"
     CDS             complement(1632..2645)
                     /locus_tag="VFY96_07245"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5606291.1"
                     /translation="MKNCRAIKFFACFNWLIVCAVPVWGQANFYEGKTVTVLIGAKSG
                     SLEIAAQIVSHHLGKYLPGKPAVILQHMPGAAHLLATNNVFNVAKPDGLTILAANPNV
                     AIAQLSKVEQVRFDVRKFQWLGSSGADGVMFSIRPDLPYKSADEIKKADRELIAGTTG
                     PGSNAHDFPLLLKEFAGFKLKLVPGYPSNSDVLLALERKEVDAWSALATTIKLAADRG
                     AVRPLVRGRVALRGFENLPVDEDLATSALGKSLMAIKSVPGAIGRAFAVAPATPSDRI
                     AQLRDAFAKAIKDPELIEEAKKAKIEVQYISPEQVLKDFNTLMNQTPETLKEMGKYIK
                     AES"
     gene            2843..3169
                     /locus_tag="VFY96_07250"
     CDS             2843..3169
                     /locus_tag="VFY96_07250"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX5606292.1"
                     /translation="MSMKQTCFVCQNVDCLSRGSEKLLNELTQKVAERSLDAEVKPYI
                     CFGGCDFGPNIVIHPQKNWYAGVKPEDLSEIVESLAGGPPVTRLDTIDPTLKEIVYSL
                     LDTGAF"
BASE COUNT          806 a          949 c         1032 g          823 t
ORIGIN      
        1 gtccgagggc ttttcttgcg attgagaatc acacgcgctg ctgatacgta agaaagaatt
       61 ctctttcagg agaacttcat ggccaaactg gttttggccg caggcgtgcc ccatccgccg
      121 cggcttgtct tcgagatgca acaagcgccg ggcaaggtcc gcggcgaact gctgatgaag
      181 caggttcggg agcaggtgga aaaggccgag cccgatgtca tcatcgaagt cgacagcgat
      241 catttcgtta actttttcta caacaaccta ccttcttttt gcatcggcat ggcggaagaa
      301 gcgcagggac cgcaggaaga ttggtgtcca atgccgcgct atacggtgca cgggcatgtc
      361 ccgtttgcca aggcgctcta caagtacggc gtgcatgaca aattcgatct cgccgcggcg
      421 cacgagctaa gactcgatca ttcgttgacg gtcccgctcc attttctcaa cccagacatg
      481 cggcttccgg ttgtaccgat ttatacgaat ggctttgcat cgccgctgcc gctcgcgacg
      541 cgctgcgtgg cgttggggcg catggtacgt cgattcattg aggcatggga aggcaacgag
      601 cgcgtcgcat tgatcgccag tggctgtttt gcgatggacg tcggcggacc cttgcgcggc
      661 tggatcgata cggagtgggc agagacgatt tcgcagctgc ttgtgcgcgg gcagtatcag
      721 acgttagcgc ggcgcgcgac cgaggagcgc atcaacgccg ctggaaacaa cagcggcgag
      781 ctcttaaact ggatcacggt cgcaggtgcg gtcggaaaaa ccaagccgct ttttttggag
      841 cccgacgaag gcaatggtta tgcagtttgg aaattcgaat gagctggaaa tttgccggca
      901 gatcgcccgc agagaatgaa aatcaagttc agttggatgt tttcgattcg atttccggcg
      961 aatctgttat tcccgcatgt ttttagcggt ggttcgactg tgctcaccat cggcggaatt
     1021 cggactggag cctcgattaa agccatcggg ggtgacggta tgacgtattg ctaatcagtc
     1081 agttgtcctt ctcaaaggag acgcgactga cacgaaggtt tggtaaggtc gttctgcgca
     1141 tttctagaac atgagcgttt atcagattaa taagctgctt tacctcaccg ataacgatcc
     1201 ggcctttcgc aaacggatgc aagaggagcc ggaaaaagtt ctcagcgaat tcaaattgac
     1261 ggccgaggag cacgctgcat tgacgtcagg cgcggttggg aagctctatc aaatgggcgt
     1321 gcacacgttc ttgctgaatc acctgtatcg ctacgagctc ttcggcgtca atcgggacaa
     1381 ttatttgagc cggattcgcg aaggcatgcc atatgatcgt cgcttcgagc agggcaatat
     1441 gccggtgcag cggttcatca agacgaaaga gtgactacgc aacggtgtgc gaggctgagt
     1501 gacattctga ggagcaatga gcgacgaaga atcgctgtgg acgcttcgcg ttagctcgcg
     1561 ctgacccgct aacgcgcact cacttgcgtt cagggtgagt cgtcgatcgg actctccgag
     1621 cgaccgacga ctcagctttc tgctttgatg tacttgccca tctctttcaa ggtctcgggt
     1681 gtttgattca tcagcgtgtt gaaatccttg agcacttgct cgggcgatat atattggact
     1741 tcgatcttgg ctttcttcgc ttcctcgatc agctccggat ctttgatcgc cttggcgaag
     1801 gcatcgcgca attgtgcgat gcggtcgctg ggggtggccg gcgccaccgc gaaagctcgg
     1861 ccgattgcgc cggggaccga tttgattgcc atgagggatt ttcctagggc acttgttgcc
     1921 aggtcttcat cgacaggaag attttcaaag ccgcgcaaag cgacgcgtcc gcgaaccaag
     1981 ggccgcacgg cgccgcgatc ggcggcgagc ttgatcgtgg tcgccagagc tgaccaggcg
     2041 tccacttcct tgcgctccag ggccagcagc acatcgctgt tggacggata gccgggcacg
     2101 agtttgagtt tgaatccggc gaattccttc agcagcagcg gaaaatcgtg cgcgttggaa
     2161 cctggcccgg tggtgccagc gatcagttcg cgatcggctt ttttgatctc gtcggcgctt
     2221 ttgtacggca agtccggcct gatggaaaac atcacgccat cggcaccgga cgacccgagc
     2281 cattgaaatt tccgcacatc gaatcgcacc tgctcgacct tggaaagttg ggcgatggcg
     2341 acattcgggt tggccgccag aatggtcaat ccatccggct tggcaacatt gaaaacattg
     2401 ttggtggcca gtaaatgagc cgcgccgggc atatgttgca ggattacggc gggcttaccc
     2461 ggcaaatatt tgcccaaatg atgggagacg atctgcgcag cgatctcgag actgccgctc
     2521 ttggctccga tcagcaccgt gacggtcttg ccctcgtaaa aattcgcttg cccccaaacc
     2581 ggcacggcgc acacgatgag ccagttgaag caagcgaaga attttatagc acggcagttt
     2641 ttcatagacc ctcctctcag ttaatggagc aggcgtttgt cagacgcttc atcaagcgct
     2701 tacaacaagc cgcccgacaa aaccgccgca aacgtatgtg atggaaccat ccgagtcaag
     2761 gatctttggt caatcaggtg aataaattaa tagcaagttg aatcggtgcc gcgagttgcg
     2821 taaaaagaga ttgaaaggtt gtatgtcgat gaaacagacc tgctttgtat gccaaaacgt
     2881 cgattgcttg agccgcggct ccgaaaaact gctcaacgag cttacccaga aggtcgcgga
     2941 acgatctctg gacgctgagg tgaaacctta catctgcttc ggcgggtgcg atttcggccc
     3001 caatatagtt atccacccac aaaagaactg gtacgccggc gtcaagccgg aagacttgtc
     3061 ggaaattgtc gagtctctgg ccggcggacc gccggtgacg cggctcgata ctatcgatcc
     3121 gaccttaaaa gaaatcgttt actccctgct cgataccggc gctttctagc tcgacgcggc
     3181 ctcggcgccg gaaccggtta aaaaagaaag atcgcttcct cccgtcgaaa tgactcttgt
     3241 ttgttgtatc tgaacgacct atcggacgcc aggatagcac cacttgtatc ggagctgcgt
     3301 ctgcagattc cctgcctaga tctggcccat cgtaattcgc cccttcactc gtcccgttat
     3361 tgtcgtctcc aacaagatcc gctggctgag ctttacgcct ggttgacagt cgtggaagca
     3421 aagcgctatg caggcaacaa aaccataaag atgaacggct atggccaaga tcgatcgaac
     3481 ggatagcgtt gagctactca accacgatat ggcggagaaa aatctttccg gttattggag
     3541 cctcgggatg gagggattgc catcggagcc catgacttcg gtcgagccgt gtctctggaa
     3601 atggcgcgat
//
LOCUS       DASULZ010000351         4907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_753693, whole genome shotgun sequence.
ACCESSION   DASULZ010000351 DASULZ010000000
VERSION     DASULZ010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753693"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..971
                     /locus_tag="VFY96_07255"
     CDS             138..971
                     /locus_tag="VFY96_07255"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX5606293.1"
                     /translation="MKIDPDKCVACGNCVPICPMGAIAIEPAIGRAVIDNDACVECFT
                     CYRGMSKEHLNPRMVRAVRRVAKIFRFRFDPEPDICPTDAIVPQELAWPRIVRRAFSD
                     PQVPHESTGIHGRGTAEVKTNDVTQRVKDGEAGFVVEFGRPGVGARFGDIQRVTTVLA
                     QLGVEFEENNPVASLMSDRSHGLIRQDILKEKILSAIVEIKAPIERVPALLQKIKDLA
                     GEVDTVISVGVSTRCDSAGNDVLGEMLRQEGYPAYRGKTNLGLGRGNAAENSQNAEAS
                     K"
     gene            972..1673
                     /locus_tag="VFY96_07260"
     CDS             972..1673
                     /locus_tag="VFY96_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606294.1"
                     /translation="MTNTLHRYSEHYASAVPRHPEPIRDDYIVFAMASRGINDDDLVE
                     KYRTFLRLALKHQPVNIGDATKGGSVRPSQDLNPSAHWRRDQQPDPEQVIAEIEGHTT
                     VAAVFDNYTAMENFVEELKAADLGISINISAPIDEAKRCCDDAGIVRHSVEYSVGFSG
                     RVDKLPDATTLELSTMCGHGMIAANFAKKMLDWVREDRRTPEEAARYMARFCSCGVFN
                     IARAERIMKRACNRK"
     gene            1694..2677
                     /locus_tag="VFY96_07265"
     CDS             1694..2677
                     /locus_tag="VFY96_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX5606295.1"
                     /translation="MKKIAVLIGIVSITLSLWPARLHAQQPISMRLGHVGFPGSLFAI
                     TADEFAKRVNTSLQGKVEIKVFHSSQLGSDEQMSRGIKVGAPEMFVPSTVMSTVEQKY
                     GVFEMPYIIVSRAHMKKVAENKEVQKQLFDGLPAKGLRVLGVWENGFRHITNNVRPIV
                     KPDDLKGIKLRVPGGVWRVKMFKIYGANPSPMPLAEVYSALQSAVMDGQENPFPQIWS
                     AKFHEVQKFLSLSGHVYTPAYLVVSEEVWSRIPKDAQATLSKISWEMGDFARSEGERL
                     DKDLMGKLAPPMKANEVDKDAFIKASSAVYEEFGKEVSGGSDLVKVIQSLR"
     gene            2836..3306
                     /locus_tag="VFY96_07270"
     CDS             2836..3306
                     /locus_tag="VFY96_07270"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX5606296.1"
                     /translation="MVLLLVLMVLLVSIGVFYRYVLNASLSWYDEFASFLLVWLTFYG
                     AVVAHYKHRHIGFEIVVDRFTPGARKIVELIAESCVLGFQAVLFYHGWLLMKRMGDET
                     TVSLVWVKMRWIYSVLPITGGLMFVISLMRFVSIAMGTESAKEEDGAWSGSSSQ"
     gene            3282..4559
                     /locus_tag="VFY96_07275"
     CDS             3282..4559
                     /locus_tag="VFY96_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX5606297.1"
                     /translation="MEWLILAVVILLTLINVPIGFGLSIAAIIFLLFKGTAPLSVVPL
                     NLFSGASSFPLLAIPLFILAGGLMDTSGISLRLVNMANSLVGFIRGGLAMVTVVATMI
                     QSEISGSSVADAAAIGNVVIPAMESRGYSRTLSAAIVSNAASAAILIPPSIPMIIYAW
                     MAEVSVAKLFFAGFFPGCIACGAMMALCYYYARKYNLPVEQKFSLREVGRSTIQAFWA
                     LVIPIVIWGGIFLGIATATEVAALAVLAALLIGVFIYRELPLRELHRILQDSVSQTAV
                     VMLIVASSAVLGWYLTSEQIPQKFARMILETTSNKYLILLFLNLFFFIAGMFLHSAAA
                     IILIVPIVMPLIRQIGVDPVHFGIIVTINLGVGQQTPPVATVLFTTAAIARVSFWEVF
                     KQGWPFTLVLALVTLLVTYVPWISLVAVDLFHW"
     gene            4678..>4907
                     /locus_tag="VFY96_07280"
     CDS             4678..>4907
                     /locus_tag="VFY96_07280"
                     /inference="COORDINATES: protein motif:HMM:NF020905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1989 domain-containing protein"
                     /protein_id="HEX5606298.1"
                     /translation="MKILSESIIKSNTGRAFEVKKGQIIRVIGVSTADYVVFNLRNVK
                     ERFDQARTKVDQGKIYVTTGDVLISKFNNVMQT"
BASE COUNT         1043 a         1306 c         1394 g         1164 t
ORIGIN      
        1 acttgagctc ctcggcgcac tcgctggcgt cgagatggcg cttaaaagtt tcggtgttaa
       61 aattctcgtg ggtgctggcc tgaccgcggc ggaaacttac ctactggaaa ccgcttccgc
      121 cgattaagtg gagggcaatg aaaatcgatc ccgacaaatg cgtggcctgc ggcaactgcg
      181 tgccgatttg cccgatggga gcgatcgcca tcgagccggc gatcgggcgg gcagtcatcg
      241 ataacgatgc atgcgtggag tgtttcactt gttatcgcgg catgtccaag gagcatctga
      301 atccgcgcat ggtgcgcgcg gtgcgccggg tcgctaaaat cttccgtttc agattcgacc
      361 ctgagccgga tatttgcccc accgatgcga tcgttccgca agagctcgcc tggccgagaa
      421 tcgtgcgccg cgccttctct gatccgcagg tgccacacga gtccaccggt atccacggcc
      481 gtggcaccgc agaggtaaaa accaatgatg tgacccagcg ggtgaaggac ggcgaggcag
      541 gatttgtcgt cgaatttggg cgcccgggag ttggcgctcg cttcggtgac attcagcgcg
      601 tcaccaccgt gctcgcccaa ctcggcgtcg aatttgagga gaacaatcca gtagcttcgc
      661 tgatgagcga tcgttcccac ggactgattc gccaagacat tcttaaagaa aaaattcttt
      721 cggccatcgt tgagatcaaa gcgccgattg agcgagtgcc tgcgctgctg caaaagataa
      781 aagatcttgc cggcgaggtg gatactgtga ttagcgtcgg tgtctccact cgctgcgact
      841 cggcgggaaa cgatgtgctt ggcgagatgc ttaggcagga aggatatccc gcctatcgag
      901 gaaagaccaa tttgggcctt ggccgtggaa acgcggcgga aaattcgcag aatgcagaag
      961 ccagcaaata aatgaccaac actctgcacc gctatagcga acactatgct tctgctgtcc
     1021 cccggcatcc ggaaccgatc cgcgacgact acattgtatt tgcgatggca agccgcggga
     1081 tcaatgacga cgacctagtt gaaaagtacc ggacctttct gcgcctggcg ctcaagcatc
     1141 aaccggtcaa catcggcgac gcgaccaagg gcggaagcgt acggccaagt caagatttaa
     1201 atccatcggc ccattggcgc cgcgatcagc aacccgatcc cgagcaggtc atcgccgaga
     1261 tcgaaggaca cacgaccgtg gctgcggtct tcgacaatta cacagccatg gaaaattttg
     1321 tcgaagagct taaagccgct gacctgggca tctccataaa catcagcgcc cctatcgacg
     1381 aggcaaagcg ctgctgcgat gatgccggga tcgtccggca cagtgtggag tactctgtcg
     1441 gttttagcgg acgagtggat aagctcccgg acgcgacgac tttggagctt tccaccatgt
     1501 gcggccacgg gatgattgcg gccaatttcg cgaaaaagat gttggattgg gtaagagaag
     1561 accgtcggac tcccgaggag gcggcgcgct atatggcgcg cttctgcagc tgcggtgtgt
     1621 ttaacattgc cagagcagag cgcatcatga agcgtgcgtg caatcggaaa taggacccgg
     1681 agcggagtaa accatgaaaa aaatcgccgt gctcattgga atcgtttcaa taaccctaag
     1741 cctctggccg gcgcggcttc acgcgcagca gccgatctcg atgcggcttg ggcacgtggg
     1801 ttttcctgga tcgctcttcg ccattaccgc cgatgagttt gccaagcggg tgaatacaag
     1861 tttgcaaggc aaggtggaga tcaaagtctt tcactcgagc cagcttggca gcgacgaaca
     1921 aatgagccgc ggaattaaag tcggcgcgcc cgagatgttc gtgccgtcca ccgtaatgag
     1981 cacggtggaa caaaagtacg gcgttttcga gatgccctac atcatcgtca gtcgcgctca
     2041 tatgaagaag gtggccgaga acaaagaagt tcagaagcag cttttcgacg ggctgcccgc
     2101 gaaaggtctg agagtgctcg gggtttggga aaatggcttt cgccatatca ccaacaatgt
     2161 gcggccgatc gttaagcccg acgatctcaa aggcatcaag ctgcgcgtgc cgggcggagt
     2221 atggagggtc aaaatgttta aaatctacgg cgccaatccg tcgcccatgc cgctcgccga
     2281 agtctattcg gcgttgcaat cggccgtgat ggatgggcag gaaaatcctt ttccgcagat
     2341 ctggtcggcc aagtttcacg aagtgcaaaa gtttctctcg ctgagcggcc atgtatacac
     2401 ccccgcttat ttagtggtca gcgaggaggt gtggagcagg attccaaagg atgcgcaagc
     2461 cacgctttcc aagatcagtt gggagatggg agactttgct cgctcggagg gcgaacggct
     2521 ggacaaagat ctgatgggca aacttgcccc gccaatgaaa gctaatgagg tcgataagga
     2581 cgccttcatc aaggcatcga gcgccgtcta tgaagagttc ggcaaagagg tctcgggcgg
     2641 gtcggatctg gtcaaagtga tccagtcgct gcgctgagcg ctaactccag atttgatctg
     2701 atcgccgcga gctgagcggc ccgggttgac gtcgttcgag aaccgtagtc attctcacca
     2761 tagactccct tagctccttt gcatttccac ctgaataaat attttcgccg cctcatcgac
     2821 ttggttgaag cctgggtggt actccttctc gtgctgatgg ttttactcgt cagcatcggc
     2881 gtattttacc gttacgttct caacgcgtca ctttcttggt atgacgagtt cgcatctttt
     2941 ctgctcgtgt ggctaacttt ctacggcgcg gtggttgccc actacaagca ccgtcatatc
     3001 ggcttcgaaa tcgtcgtcga ccggtttacg cctggcgcga gaaaaatcgt cgagttgatt
     3061 gccgaatcct gcgtcctcgg ttttcaggcg gtgctcttct atcatggctg gttgctcatg
     3121 aagagaatgg gcgatgaaac caccgtctcc ttagtttggg ttaagatgcg ctggatttac
     3181 agcgttctgc cgatcactgg cggcttgatg ttcgtgatca gcctgatgcg gtttgtcagc
     3241 atcgcgatgg ggaccgaaag tgcgaaggag gaagatgggg catggagtgg ctcatcctcg
     3301 cagtagtcat tttactcaca ctcatcaacg tccccatcgg cttcggcctt tccatcgccg
     3361 cgattatttt cctgcttttt aaaggcacag cgcccctgag cgtcgtgccg cttaacctct
     3421 tttccggcgc ctcgagcttt cctcttctgg ccattccgct attcatatta gcgggcggtt
     3481 tgatggacac ctcgggaatt tcgcttcgcc tcgtcaatat ggccaatagc ttggtagggt
     3541 ttattcgcgg cggactggcg atggtgacag tagtggcaac gatgattcag agcgaaattt
     3601 cgggctcatc ggttgccgat gccgctgcca tcggcaacgt ggtgatcccg gccatggagt
     3661 cgcgcggcta ttcgaggact ttgagcgcgg ccatcgtctc caatgcggcg tcggcggcaa
     3721 ttctcattcc gccttcgatc ccgatgatca tttatgcctg gatggctgaa gtgtcggtag
     3781 ccaagctctt ttttgccggg ttttttcctg gttgcatcgc ctgcggcgcc atgatggcac
     3841 tgtgctacta ctacgcacgt aaatacaatc tgccggtgga gcaaaagttc tctctgcgcg
     3901 aggttggtcg atcgacgatc caagcctttt gggcgttggt gattccgatc gtgatttggg
     3961 gcggtatctt tcttgggatt gccacggcca cggaagtcgc ggccctcgca gtgctcgccg
     4021 cgctgctcat cggcgtgttt atctatcgcg agcttccgct gcgcgagctt caccgcatcc
     4081 tacaggactc ggtatcgcag accgcggtgg ttatgctgat cgtcgcttcc tcggcagtcc
     4141 tcggctggta tctcaccagc gagcagatcc cgcaaaaatt cgcgcgcatg atcttggaaa
     4201 cgacctcgaa caagtacctg attctcctat tcctcaatct ttttttcttt attgctggaa
     4261 tgtttctcca ttcggctgcc gcaatcatcc tcatcgttcc gatcgtgatg ccgctgatcc
     4321 ggcagatcgg tgtcgacccg gttcattttg gcattatcgt gacgatcaat ctcggtgtgg
     4381 gacagcaaac tccgccggtg gcgacggttt tgttcactac ggctgcgatt gcgcgggttt
     4441 ctttctggga ggtctttaaa caagggtggc cttttaccct cgtgctcgct ttggtcacct
     4501 tgttggtgac ctatgtgccc tggatcagcc tggtagcggt tgatcttttt cattggtgac
     4561 agacttcatt acacgctcca gaatcggcaa gtaaaatgcg cgctcgcgcc ttgatcttgc
     4621 cgcgatgcat cggctaattt accaggcaca actacgactt taattgcgga ggacagagtg
     4681 aagattctca gcgagtcgat catcaagagc aacacaggga gagcttttga ggtcaagaag
     4741 ggtcaaatca ttcgcgtcat cggcgtgtcg accgcggatt atgttgtttt taatctgcgc
     4801 aacgttaaag aacgttttga tcaggcgcgc accaaagtcg atcagggaaa gatctacgtc
     4861 accacgggag acgtgctcat ttcgaaattc aacaatgtca tgcagac
//
LOCUS       DASULZ010000352         2280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1160374, whole genome shotgun sequence.
ACCESSION   DASULZ010000352 DASULZ010000000
VERSION     DASULZ010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160374"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            315..1283
                     /locus_tag="VFY96_07285"
     CDS             315..1283
                     /locus_tag="VFY96_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEX5606299.1"
                     /translation="MKAIRIHNHGGPEVLAYEDVPVPEPRAGEARVKIEASGVNFIDI
                     YHRIGLYPMKRPCILGSEGAGIVDAVGEGVTEIKKGDRVAYAMVPGSYAEYAVVPAIR
                     LVPVPDDIDAKSAAGLMLQGMTAHYLTHSTYPLQKGETALVLAAAGGVGLLLVQLAKM
                     LGAKVIGTVSTETKADLARRAGADEIILYTQADFLAEVKRITDGQGVHVVYDSVGAST
                     FEKSLDCLRPRGYLVLFGQSSGPVAPIDPAKLAAKGSLFLTRPSLAHYTLTRSELLRR
                     ATDLFDWMQAGKLNLRIEKSLPLQDAREAQQLLEKRKTTGKLILIP"
     gene            complement(1273..1836)
                     /locus_tag="VFY96_07290"
     CDS             complement(1273..1836)
                     /locus_tag="VFY96_07290"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEX5606300.1"
                     /translation="MRKLVNVMVLAFALGWVVCASAADGENSRPSIPDEVGDAWDRLQ
                     QVLQDWSDRFRERFGARENVENRPAISEMLSRKEALGLSPDQVKKIEQLRDNFERLSI
                     RNNAELRIVELDIAGLLDNPAVDVAKVEAKVREAEKLRADLRIARIKVIEQAKAVLTP
                     EQRKKFYDTVESRSPRAARGQNPSAKE"
     gene            complement(1872..>2280)
                     /locus_tag="VFY96_07295"
     CDS             complement(1872..>2280)
                     /locus_tag="VFY96_07295"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX5606301.1"
                     /translation="KARSHRLMAMFGVDWRDASRFDLVVNLGRMGIPTIKHLILETLR
                     QPDYQITPGSKQAFEDFALASQVRAVLAMSTDATRTRLDVKASAGNVAVSGSMPSWVS
                     EETLVTKIKTIPGVKDVQTDIVNVPDLGVVDWP"
BASE COUNT          514 a          641 c          616 g          509 t
ORIGIN      
        1 gacatgccta agatcaaaag gttaaatagg cccgtgctca gaatagccaa atccgaattg
       61 ccgaactcca ccgaggtttt agtaatgcct tggagacaga aataatcgta ctttccatcc
      121 caacctctac gtgaatcaaa aaatctccca taccggtcct cagaacttcc aggcagccag
      181 gttcatttct gttttcaacc acaaaaaggg ctagcttccc gcatgcgtca aaatgacacg
      241 aaaggctgaa gcattcgcta aattgaattc ttcttgctaa aagcgctaag cggtgattat
      301 ccgcgagggt agtcatgaaa gccattcgga ttcacaatca cggcggcccg gaagtattgg
      361 cctacgagga tgttccagtc ccggagccta gggcgggaga agcgcgcgtc aaaatcgagg
      421 cgagcggcgt caatttcatc gatatttatc atcgcatcgg tctctatccg atgaagcgac
      481 cctgcattct tgggagtgag ggagcaggga tcgtcgatgc agtgggcgag ggcgtcaccg
      541 aaatcaaaaa aggtgatcga gtcgcttacg cgatggttcc gggatcatac gccgagtacg
      601 ccgtggtacc ggcgatcagg ctcgtgccgg tgcctgacga tattgatgcg aaatcagccg
      661 cgggcctgat gctgcagggc atgacggccc actatctcac ccatagcacc tatccattgc
      721 agaaaggtga gaccgcgctc gttctcgcgg cggctggagg agtgggcttg ctcctcgttc
      781 agctcgctaa aatgcttggc gcgaaagtga tcggtacggt ttccacggag accaaagcgg
      841 atctcgctcg cagggccgga gcggacgaaa tcatcctcta tacgcaggcc gattttctcg
      901 ctgaagtgaa gcgcatcacc gatgggcaag gcgtccatgt ggtgtacgac tcggtgggcg
      961 cgagcacgtt cgagaaaagc ctcgactgtc tccgaccgcg cggctatctg gtgctgttcg
     1021 gacagtcgag cggaccggtc gcaccgattg atccggcaaa attggccgcc aaaggctcgc
     1081 tctttctcac ccgcccgagc ctagcgcact atacattgac tcgttcggag ctgttgcgtc
     1141 gtgccaccga tctgtttgat tggatgcaag cgggaaagct caacttacgc atcgaaaaaa
     1201 gcttgccgct gcaagacgcg cgtgaagcac aacagctcct cgagaaacgt aaaactacag
     1261 gcaagctgat tcttattcct tagctgaagg gttttggcct cgtgctgctc ggggtgagcg
     1321 cgattcgacc gtatcgtaaa atttttttcg ctgctccgga gttaataccg cttttgcctg
     1381 ctcgatgacc tttatccgag cgatgcgtag atccgcccgt agtttttccg cctcacgcac
     1441 tttcgcttcg actttggcaa catcgacggc ggggttatcc agtaaaccgg cgatatccag
     1501 ttccacgatc ctgagctcgg cgttgttacg gatgctcagg cgttcgaagt tgtcgcgtag
     1561 ttgctcgatc tttttcacct gatcgggcga aagccccaac gcttctttgc gactgagcat
     1621 ttcggagatt gccgggcggt tttccacgtt ttctcgggca ccaaagcgct cgcggaaacg
     1681 atcgctccaa tcttgaagaa cttgctgaag gcgatcccag gcatcgccga cctcatcggg
     1741 aatggagggg cgcgagtttt cgccgtcggc tgcgctcgca caaaccaccc atccgagcgc
     1801 gaatgccagc accatcacgt ttacgagctt ccgcatacgc aaatcgttgg atcgagactc
     1861 gatctaatcg atcacggcca atctaccaca ccgagatccg gcacattgac gatgtccgtc
     1921 tgcacatctt tcacaccagg aatcgttttg atttttgtca ccagggtctc ctccgacacc
     1981 caactgggca tcgagccgga gactgcgaca ttgccagccg aggctttgac gtccagacga
     2041 gtccgcgtgg cgtccgtcga cattgccagt accgccctga cctgagatgc caaagcaaaa
     2101 tcttcaaacg cctgtttcga accgggcgtg atctgataat ccggttggcg cagcgtttcc
     2161 aagatcaaat gtttgatggt gggaatgccc atgcgcccga gattcacgac gagatcaaac
     2221 cggcttgcat cgcgccagtc gacaccgaac atcgccatga gtcggtggct gcgcgctttg
//
LOCUS       DASULZ010000353         2570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1160429, whole genome shotgun sequence.
ACCESSION   DASULZ010000353 DASULZ010000000
VERSION     DASULZ010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160429"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /locus_tag="VFY96_07300"
     CDS             complement(<1..1296)
                     /locus_tag="VFY96_07300"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEX5606302.1"
                     /translation="MHHQVTLLSLLFAALVAFLVLQNQSAFSQAHRSRAERFRHNGAV
                     SDEDRPVIDDPKSDATEAPTGFDNLTNGFIEQGPPYETLTAENVVARRSFNDNRFIFS
                     EVETIQDGLGPTYNAQSCRECHQNVVTGGASQVAELRSGRLENGQFFESLGGTLIQSR
                     ATHPDIVERVIPEDAIRTFRISTNTLGNGFVEAIANSTLLAIRDRQPADMRGTAVIVP
                     VLEAAGTSRIGRFGWKGQHASLESFSADAYLNEMGITSPLFPEENTSRDRFVGFGSGY
                     DLVADPEDDGEDIVAFADFMRSTKAPPRGPISSDVLAGEALFNKIGCAVCHTPSITTA
                     KPGAKINGATFNVPAALGNKIIHPYSDYLLHDIKTGDGIPVLPLPEYSSTANQIRTAP
                     LWALRTRNRLMHDGLSFTKEDAIQRHGGQAAGVTNNYNAL"
     gene            1671..2324
                     /locus_tag="VFY96_07305"
     CDS             1671..2324
                     /locus_tag="VFY96_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5606303.1"
                     /translation="MRILIIEDEPRLLRNLARALREEGYAVDVAAAGDEGLYKAESYN
                     FDAIVLDVMLPRLDGWEVLERLRKQKQTPVLMLTARDAFKDRVRGLDDGADDYLVKPF
                     DLSELLARLRALIRRSAGQTTSQIELGGVVIDIRARSINREGCQIPLTAREYAIFEYL
                     ALHRGEVVTRTALYEHLFDENEDTMSNLLDVHIFSIRKKIGRELIKTRRGQGYSIDR"
     gene            2360..>2570
                     /locus_tag="VFY96_07310"
     CDS             2360..>2570
                     /locus_tag="VFY96_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606304.1"
                     /translation="MQLWLAFLLVCLLSGFGLTVYQLERISQLSQLDGELERRVAVIS
                     GAIRGGAPKTGVSPGSFERRPENREL"
BASE COUNT          571 a          663 c          725 g          611 t
ORIGIN      
        1 gagcgcgttg taattgtttg tcacgcctgc cgcctgaccg ccatggcgtt gaatcgcatc
       61 ctcttttgta aaagacaatc catcatgcat cagccgattg cgagttcgaa gcgcccacaa
      121 cggcgcggtt cggatctgat tagcggttga actatattcg ggcaaaggca agaccggtat
      181 gccgtcgcct gtttttatgt cgtgcagcaa ataatcgctg tagggatgga tgatcttgtt
      241 accgagcgcc gctggcacat taaacgtagc gccgtttatt ttagcgccgg gcttcgcggt
      301 ggtgatcgaa ggcgtatggc acacagcgca gccgattttg ttgaataacg cttcaccggc
      361 gaggacgtca ctcgatatcg gtccccttgg cggcgccttg gtcgaccgca taaaatccgc
      421 gaaggctaca atatcttcgc cgtcgtcctc aggatcagcc accagatcat aaccggagcc
      481 gaaaccgaca aagcggtccc tcgaagtgtt ctcttctgga aacagcggcg acgtgatgcc
      541 catttcattg agataggcat cagcggaaaa cgattctagg ctggcgtgct gacctttcca
      601 gccgaagcgg ccgatacggg aggtcccggc agcttcaagc acggggacta tgacagccgt
      661 tcctcgcatg tctgcaggtt gtctatcgcg aatggccaac agagtgctgt tcgcgatcgc
      721 ctctacaaaa ccattgccca gggtattggt cgagatgcgg aaagttcgga tcgcgtcctc
      781 ggggatcacg cgctccacga tatcggggtg tgtggcacgc gattgaataa gcgttccgcc
      841 caacgactcg aagaattgac cgttctccaa ccgcccggag cgaagctcgg caacttgact
      901 ggcgccgcca gtaaccacgt tttggtggca ctctctgcaa ctctgggcgt tgtatgtggg
      961 accgagtccg tcttgaatcg tttcaacctc cgagaagata aaacgattgt cgttgaaaga
     1021 gcggcgcgca accacgttct cggcagtgag cgtttcgtaa ggaggtcctt gctcgatgaa
     1081 gccgttcgtc aagttgtcaa aaccggtggg agcctcggtg gcatcgcttt tcggatcgtc
     1141 tattaccggg cgatcttcgt cgctaaccgc gccattgtgg cgaaaccgtt ctgcacgact
     1201 ccggtgtgcc tgagagaagg cgctttgatt ttgcagaacg aggaaagcga caagcgcagc
     1261 gaatagcaga ctgagaagag tcacttgatg gtgcatagtc cactcctttt tacgattttg
     1321 atgcctcgcc aaaaattgct gcccgttgaa cacgacgaca gccgctcggc gttacgcgcc
     1381 gaactagctt tgccgaaaac gttttaacga gacggcgcga cttggatcgg gcttgatgtc
     1441 caacttgccg ggcgagagcc agcaatttgc tgcggctaaa atacgaaaaa cttgattaag
     1501 ttaggcttaa ggccaaatta atcttgcccg agatcgtgga aacgctttac tgaaaaatcc
     1561 ttaatccttc cttaatgtgc gatgtgacac gatgtaaggc tcgataatcg tttgtcgaag
     1621 tttcattggg tcaacaaata tgttggaatc agccgcagtg ttcgacggtg atgaggatcc
     1681 taattattga agatgagcct cgactgctcc ggaatctcgc ccgagcttta cgggaggaag
     1741 gttacgccgt agatgtggcc gcagcgggtg atgaaggtct ttacaaggcg gagagttaca
     1801 atttcgacgc gatcgtgctc gatgttatgc tgccccgtct cgatggctgg gaagtattag
     1861 aaagactgcg aaagcaaaaa caaactccgg tgctgatgct cacggctcgg gacgccttca
     1921 aggaccgcgt tcgcggtctg gacgacggag ccgacgatta tctcgtcaaa ccatttgatt
     1981 tatccgaact gctggcgcga ctccgggcat tgatccgtcg ctcggctggc cagaccacgt
     2041 cgcagataga actcggtggc gttgtcatcg acatccgcgc tcgctcgatt aatcgcgagg
     2101 gctgtcagat tccattgacg gctcgcgagt acgcgatttt cgagtatctc gcgctacacc
     2161 gaggagaggt cgtcacgcga accgcgctct atgagcatct tttcgatgag aatgaagata
     2221 ccatgtctaa tctgttggac gtgcatatct ttagcatccg taaaaaaatc gggcgtgagc
     2281 ttatcaagac tcgtcgcggc cagggttact cgattgaccg ctgatcatta tcatgttgtt
     2341 cagatcgatt cgttggcgct tgcagctgtg gctggcgttc ctgcttgttt gcctgttgag
     2401 cggtttcggt ctcaccgtct atcaacttga acggattagc caactgagcc agcttgacgg
     2461 ggagttggag cgtcgcgttg cggtgatcag cggcgctatt cgtggaggcg cgccgaaaac
     2521 aggtgtttcc cctggctcat ttgagagacg cccagagaac cgcgaactca
//
LOCUS       DASULZ010000354         3325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_246667, whole genome shotgun sequence.
ACCESSION   DASULZ010000354 DASULZ010000000
VERSION     DASULZ010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246667"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1425)
                     /locus_tag="VFY96_07315"
     CDS             complement(34..1425)
                     /locus_tag="VFY96_07315"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5606305.1"
                     /translation="MTAANLSVADRLVQLLDHLKIARAHFAASMLADVTGFVQAHSER
                     IASLTLVCPPRVDRSLHVLGNRLMIIAGDQGRPATMVRDAIKNLSEATVIWLPGYFSP
                     PWADPAADCTADIASALFDVISNHQVGKNDAARGSVHGAVAGISYHSQGEGEPLVLLP
                     LSLASSQWDPLRSRLSSHYQTIVLGGPELGFVAMLESRGQSAGYRGVVQRVVDAVHLH
                     PGEVILEVGCGSGVLDRWLAEYTARANRIIGVDVNRYLVREAKALATQEGLADIITFQ
                     EGDAEALPFPDEHVDVALSFTVLEEGNAERMLAGLARVTKPGGRVAVIVRALDIPWVV
                     NVPLRPELKAKVQTPRGFVGTEGCADASLYRRFQQTGLKRVQMFPQFAAFEQPETPQA
                     QFAQGAILGALTTEETQEWHRAMAQAVADGTYFIAQPFHCAVGKKRLSKNSRGRLQRA
                     DRKCRYAREGACD"
     gene            complement(1498..2925)
                     /locus_tag="VFY96_07320"
     CDS             complement(1498..2925)
                     /locus_tag="VFY96_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007463453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5606306.1"
                     /translation="MARLQIKTRNNGEITLQEETVQKFKESLRGELIRIGEAGYDDAR
                     SIWNAVIDRQPALIASCLGVADVVTCVKFARAHGVNLSIKGGGHNISGLAVCDGGLML
                     DLSRMRGVWVDPSTRTARAQGGCLLGDVDRETQVHGLAAVLGFISNTGIAGLTLGGGF
                     GYLTRRFGWTSDSVLSMDMVTAGGSVVRASENENSDLFWALRGGGGNFGVVTSFEYRL
                     FPVGPEIIAGAIAWPAEDARRVLDMYRSLTEQAPRELTCVAALRMAPPAPWLAKEIHG
                     KPIVALFFCHTGTISEGEKLAAPIKAFGSPVGDIVQRRSYVSQQSLLDATQPKGRRYY
                     WKSEYLPTLEPELLAKTIEHARGMLSPHSVILLFHLDGELNRLSDAHSAVGNRTTRSV
                     LNIAASWEKSEDDHANVEWARAAWRDLRRFSTGGTYVNFLTEEEGDERIRAAYGNNYE
                     RLAEVKSKWDPDNLFRMNKNIAPQR"
     gene            complement(2995..>3325)
                     /locus_tag="VFY96_07325"
     CDS             complement(2995..>3325)
                     /locus_tag="VFY96_07325"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5606307.1"
                     /translation="VSATDAVECAVDIQKAFAVYNKENQETPLNLRIGVSAGEPIEEH
                     GDLFGKAVQLAARLCAHAEPAQILVAQVVFDQCEGKKLPFLDLGEITPKGFDQPIRLY
                     ELNWAGL"
BASE COUNT          702 a         1001 c          934 g          688 t
ORIGIN      
        1 cccgatcccc aagtctctgt ctctgtcggc ctatcagtca caagccccct cccgtgcgta
       61 ccggcacttt cgatcagcgc gctgcaatct accccgacta ttcttgctca ggcgtttttt
      121 gccaacggcg cagtgaaatg gctgcgcgat gaaataggtc ccgtccgcca ctgcttgcgc
      181 catcgctcta tgccattcct gcgtttcctc cgttgtgagc gcgccgagaa tagcgccctg
      241 cgcaaactgt gcttgcggtg tttcgggttg ctcgaaggcg gcaaactgag gaaacatttg
      301 tacacgtttc agacctgtct gctgaaaacg ccgatagaga ctggcatcgg cacatccctc
      361 ggtaccgacg aagccacggg gagtttgtac tttggctttg agctcggggc gaagtggaac
      421 attgacgacc cagggaatat ccaaggcacg cacgattacg gcaacgcggc caccgggttt
      481 cgtgactcgg gctagccccg cgagcatgcg ctcggcgttt ccctcttcaa gaacggtaaa
      541 cgagagcgcg acatctacat gctcgtctgg aaaggggagg gcttcggcgt ctccttcctg
      601 aaacgtgatg atgtctgcca gaccttcctg tgtcgcgaga gccttggcct cgcgcacgag
      661 atatcgattc acgtcgacgc cgatgatgcg gttcgcgcgc gcggtgtact ccgcgagcca
      721 acgatccaaa actcccgatc cgcaacccac ctccagaatg acctcgccgg gatgcaggtg
      781 caccgcatcg accactcgct ggaccacgcc gcgatagcct gccgattggc cgcgcgattc
      841 aagcatcgca acaaacccca actcaggacc gccaagcacg atggtctgat agtgcgagct
      901 gagccgcgat cgcagcggat cccattgaga ggacgctaga ctcagtggca ataacacgag
      961 cggctcgcct tcgccctgac tatgatagct aatgcccgca acggcgccgt gaacgctacc
     1021 gcgcgcggca tcgttctttc ctacttggtg gttcgagata acatcgaata aggcgctcgc
     1081 gatgtccgca gtgcaatcag cagcaggatc tgcccacggc ggactgaaat aaccgggaag
     1141 ccagattact gttgcctctg agaggttctt tattgcgtcg cgcaccatag tagccgggcg
     1201 gccctggtca ccggcaataa tcatcaaacg attacccaag acgtggaggc tccgatcaac
     1261 gcggggtgga cagacgagcg tcagcgacgc gatccgttca gaatgggctt gcacgaaacc
     1321 cgtgacgtcc gccagcatgg aggccgcaaa gtgggcacgt gcgattttca agtggtcgag
     1381 cagctgtacg agccgatcag ccacagaaag gttggccgct gtcatgagcg cacctcgttg
     1441 atgggactac atcgttggac cttacatggt cgctcttcaa tttatgatgg gtccgctcta
     1501 tcgttgcggc gctatgtttt tgttcatccg gaataaatta tccgggtccc attttgattt
     1561 gacttcggcc aaccgctcat agttgtttcc gtaggccgcg cgaatgcgct catcaccctc
     1621 ctcttcggtc aggaagttca cataggtgcc gccggtggag aaacggcgca gatcgcgcca
     1681 tgcggcacgc gcccattcca cgttcgcgtg gtcgtcctcg ctcttttccc acgacgccgc
     1741 gatgttgagg accgagcgag tcgtccggtt gccgacagcc gagtgtgcat cagagagacg
     1801 gtttaactcg ccatcgagat ggaagagcag gatcaccgaa tggggcgaga gcatgccgcg
     1861 ggcgtgctcg attgtttttg ccaacagctc gggctcaagc gttggcagat actccgattt
     1921 ccagtagtag cgccggccct tcggctgggt cgcatcgaga agactctgct gtgacacata
     1981 ggagcggcgc tgaacgatat cgcccaccgg ggagccgaag gccttaattg gcgcggccag
     2041 tttttctcct tcgctgattg taccggtatg gcagaaaaat aacgcgacaa tcggtttgcc
     2101 atgaatctct ttggccagcc atggggctgg aggggccatg cgcagtgcgg caacgcatgt
     2161 gagttcccgg ggagcctgct cggtcaaact ccggtacatg tcgagcacgc ggcgagcatc
     2221 ctcagcgggc catgcgattg cgccggcgat gatttcaggc ccaaccggaa agagccggta
     2281 ttcgaagcta gtgaccactc cgaagttgcc gccgccgccg cggagagccc aaaacaggtc
     2341 gctattttcg ttttcgctcg cgcgtacaac gcttccgccg gcggtgacca tatccatcga
     2401 cagaacgctg tcagaagtcc agccaaagcg cctcgtgaga tagccgaagc cgccgcctag
     2461 agtcaggccc gcaataccgg tgttggaaat gaaacccagc actgcagcca ggccatgaac
     2521 ctgggtctca cggtccacgt cgcccaagag gcacccgcct tgcgcccggg ctgtgcgtgt
     2581 agaggggtcc acccacacgc cgcgcatgcg cgacaggtcg agcatcaagc cgccgtcaca
     2641 gacggcaagg cccgaaatgt tatgaccgcc acctttgatg gataaattga cgccatgcgc
     2701 gcgcgcaaac tttacgcaag tcaccacatc ggcaacgcca aggcaacttg cgataagcgc
     2761 gggctggcgg tcgatcacgg cgttccagat cgagcgcgca tcatcgtagc cggcctcgcc
     2821 tattcggatt agctcaccgc gcagactctc cttgaatttt tgcacggtct cttcttgcaa
     2881 agtaatttct ccgttgttgc gggttttgat ttgcaggcga gccatggtgt acctccttta
     2941 taataaagtg catggcgaga cgagcgggct aggaagtctc aacagccgca aatattacag
     3001 ccccgcccaa ttcagttcat acaggcgaat cggttgatca aaccctttcg gagttatttc
     3061 gcccaagtcc agaaacggca actttttacc ctcacattga tcgaacacca cttgtgcgac
     3121 caaaatctgc gcgggttcag catgggcaca aaggcgcgcc gccagttgaa cggccttgcc
     3181 gaacaaatca ccatgttcct caatgggctc gccggcgctt acaccgatgc gtaaattcaa
     3241 cggcgtttcc tgattttcct tgttgtacac ggcaaacgct ttctggatgt caacggcgca
     3301 ttcgacggca tcggtcgccg aaacg
//
LOCUS       DASULZ010000355         2238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_856304, whole genome shotgun sequence.
ACCESSION   DASULZ010000355 DASULZ010000000
VERSION     DASULZ010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856304"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="VFY96_07330"
     CDS             <1..947
                     /locus_tag="VFY96_07330"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606308.1"
                     /translation="SACCLAPVGAAEQVRTAIPRATLNYLSIPVAEVKGLFRDQGLEN
                     QTIVIPGSTAIAALVSGNVDYSGAGGSGMRAALRGAPIKAVMFQTEKVTWYLLAAPDI
                     QKISDLKGKKIAVGTIGDTQDTLITMLVEREGVSGRDITRIAMPSRNTTSTILSLKSG
                     AFSAAVVNADESLLGEKEGLRTLAFVGDLFPYPFQGFVATDKAIAERPNDIKRWLRAM
                     ARALMLIRDNPEEAADIALKKIPMGNVSRPLVIEGIRRFAKALPEGVPGLPSAQGIKN
                     VMEFDVKLPLKIKEDVSPDKVMNLKLMREVKEELESKR"
     gene            986..1990
                     /locus_tag="VFY96_07335"
     CDS             986..1990
                     /locus_tag="VFY96_07335"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606309.1"
                     /translation="MLRIGLKPLVLFLFTVSLLTVQSHLHAQEKKNLRVAFVSLSWNN
                     QLPIRVAMSKGFFKDQGLTLEPIFVRGGPIAITALISGNVDYASVGGAQAPIRSNARG
                     LDLQIISSMSNYTNYTLLGSKDARSVEELRGKIIGVTGAGAFSDFAIRIYLKRNNLDP
                     DKDATLRAIGGTALRALALEKGVIAAAPFSAEDTVRLLDKGFPMIVNLNEALRIPQSV
                     FVTRGEVLQKYPETTKRFLKAVILGMQFTTHNKQEAIKIGYSAGLKGEPDVVNRAYDL
                     FSPGYAADLSVAQDGIQIMLDEDIRAGVVEKNFTLDRVINDKIVKQAQQELRKEGRLK
                     "
BASE COUNT          540 a          624 c          615 g          459 t
ORIGIN      
        1 tttccgcgtg ctgcttggca cccgttggcg cggccgaaca agtgagaacc gctatccccc
       61 gcgcaacgct gaactacctg agcatacccg tcgcagaagt gaaaggcttg tttcgagatc
      121 aaggcctaga aaaccaaact atcgttattc caggcagcac cgcgatcgct gctctggtga
      181 gcggtaacgt ggattacagc ggcgccggcg gcagcggcat gcgcgcagcg ctccggggcg
      241 cgccgatcaa agccgtcatg tttcagactg agaaagtgac atggtattta cttgccgcac
      301 ccgacattca gaaaatttcc gatctcaagg gcaagaaaat tgccgtcggc acaatcggcg
      361 acacccagga tactttgatc accatgctgg tcgagcgcga aggagtttcc ggccgtgaca
      421 ttactcgcat tgccatgcct tcgcgcaata ccaccagtac gatcttatcg ttgaaatccg
      481 gcgcattcag cgccgccgtg gtcaatgccg acgaatctct tctcggcgaa aaagaagggc
      541 tccggacact ggcattcgtc ggagatctgt ttccctatcc ctttcagggt tttgtcgcga
      601 ccgacaaagc tattgccgag aggccgaacg acatcaagcg ctggctgaga gccatggccc
      661 gcgcactgat gcttatacgc gacaaccccg aagaagcggc cgatattgcc ttgaaaaaaa
      721 tccccatggg caatgtcagc cgacccctcg tcatcgaggg tattcggcgt ttcgccaaag
      781 ctttaccgga gggagttcct ggtcttccgt ccgcgcaagg aatcaagaac gtgatggagt
      841 tcgatgtcaa gctcccgctc aagatcaaag aagatgtgag cccggacaaa gtgatgaatc
      901 ttaaattgat gcgcgaagtc aaagaagagc tggaaagtaa acgctgagag aacgtcgcga
      961 atcttgagcg agtcgtgaaa tagtgatgct ccgcatcgga ttgaagccgc tcgtactgtt
     1021 cctgttcaca gtttcgctcc taaccgttca gtcgcacctt cacgcgcagg aaaagaaaaa
     1081 cctccgggtc gcgttcgtca gcctttcgtg gaacaaccaa ctgcccatcc gtgtcgccat
     1141 gagcaaaggg tttttcaaag accaaggcct gacactcgag ccgatcttcg tccgcggcgg
     1201 cccgatcgcc atcacagcgt tgatttcggg gaacgtcgat tacgcgagcg tcggcggggc
     1261 tcaggcgccg atccgcagca atgcccgcgg gctcgatctt caaatcattt cgtcgatgtc
     1321 gaattatacc aactacaccc tgctcggcag caaagacgcg cgaagcgtcg aagagctacg
     1381 cggaaaaatt atcggcgtca ccggcgcggg tgcgttctcc gacttcgcca tccgcattta
     1441 ccttaaacgc aacaacctag atcctgataa ggatgcaacc ttgcgcgcca tcggcgggac
     1501 cgcattgcgc gccctcgccc tcgagaaagg cgttatcgcc gccgcgccgt tctcggcgga
     1561 ggataccgtg cgcttgctgg acaaaggttt tccgatgatc gtgaatttga atgaagcgct
     1621 gcgcattccg caaagtgtgt tcgtcactcg gggcgaggtc ctgcaaaaat atccagaaac
     1681 gacgaaaaga tttttgaagg ctgtgatcct cggcatgcag ttcaccacgc acaacaaaca
     1741 ggaagcgatc aaaattggct atagcgccgg cctgaaaggg gaacccgatg tggtcaaccg
     1801 cgcctacgat cttttcagcc cgggctacgc cgctgacttg tcggtcgctc aagatggcat
     1861 tcagatcatg ctcgatgaag atattcgcgc tggtgtcgtg gagaaaaact ttaccttgga
     1921 ccgggtgatt aacgataaaa tcgtcaagca ggcgcaacag gagctcagaa aggaaggccg
     1981 gctgaaataa acgcactcgg cggcggcggc cggtgcccaa agcagccgtt gaccggcagc
     2041 aagctcgatt gataaggttc gcgttatggg tttgccgaac aaccccactg gtaagcaaag
     2101 catacattgc gcagacaatc gttaacctgg aggtaaatta tggcgatcgg actttcacac
     2161 ggcggcagta atgtttattc atcgcctgag cgctcgcgcg aactctgggt cggcacacag
     2221 gatggcttgg tcatgttt
//
LOCUS       DASULZ010000356         4821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_348335, whole genome shotgun sequence.
ACCESSION   DASULZ010000356 DASULZ010000000
VERSION     DASULZ010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348335"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2509)
                     /locus_tag="VFY96_07340"
     CDS             complement(<1..2509)
                     /locus_tag="VFY96_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(1952..1954),aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5606310.1"
                     /translation="MSSFLSKWPLIRQIRERKDGTGLEAMSDKTRAMHARIDDAEVAR
                     SICPYCGVGCGQLVFHKDGKLVSIEGDPESPISQGNLCPKGAASYQLLTHSRRQTKVK
                     YRAPRSKDWMEIPLQRAMDMVAERVWESRKRTFVRQENGSAINHTTSICHLGGATLDN
                     EENYLIKKLFTGGLGMVCVSNQARIUHSSTVPGLGTSFGRGGATTAQQDLANADCILI
                     EGSSMAEAHPVGFRWVMKAKERGATIIHVDPRFSRTSALADIWVPIRAGSDIAFLGGL
                     VRHIIENELFFREYVVNYTNASCILRDDYQDPEDSADGFFSGWNEGERNYSMQSWLYK
                     GEGLSFPERDFTLQDPQCVFQKLERHFARYTPEMVEKVCGIPPALFHKVADALVRASG
                     PDKTAAICYAVGWTQHSKGVQIIRTASILQLLLGNIGRPGGGILALRGHASIQGSTDI
                     PTLYDILPGYLAMPRGGAEETLQKYLDTNTTKTGLWSNTPAYFVSLLKAYYGKSATAE
                     NDFGYNWLPKITADHSFFEYLYEMADGKVEGMFLIGQNSAVGAPNSRFQRKSMAKLKW
                     FVIRDMVETEPARFWHDSAEIERGELRTEEIETEVFFFPAAGHAEKEGAFTNTQRLLQ
                     WREKAVDPPGDSRSDAWFIHQLALRLIAKARASTDPIDEPLRALDWWYPQDELGEPKI
                     EAVLAEINGWNTQPVAGRADAGGTDGILFGGVDRQGNAHRGPQVADYNELKPDGSTAC
                     GCWIYSGVFNRDGVNKANARKPKDYLGHGWGFSWPSDRRIIYNRASAKPNGEPWSERK
                     KLVWWELEKWTGIDVPDFKANKPPDYRPRGDQTGLDGQ"
     gene            2718..4196
                     /locus_tag="VFY96_07345"
     CDS             2718..4196
                     /locus_tag="VFY96_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5606311.1"
                     /translation="MDDTLQFGFPLWVRLTHFFNFFFITLLIRSGIEIIGAHPKFYWR
                     DDSLPGSEWFRLTKKRLPKNQLWTAEDEVEPMSRWTALPGENNLGLGRHWHFWAANGW
                     LLAGLIYVILLFATPQWRRLLPTSWHVFAAAWQAIVAYLHFRLPPEGNPFNAIQQLTY
                     CFIIFILSPLQIVTGIMMSPALGARFPWFPKMLGGRQAARSLHFIGLIVFSGFIVVHI
                     SLVFAHGFGRGMAKIVLGDEVSSYVLATMIGLLAIAAVIVFHIWGTRYSLVRPSQAKR
                     FLEIGIDPLRRLLFRHWHSRQEYLRVSAYSRINGKPPRNEMYERLAAANFMDWRLEVG
                     GLVEKNLSLSLNDLHQMPRQTQTTLHCCIQGWSYFAQWAGVSVSSIIDMCGPLPNARF
                     LVFHSLDEKWERPGHGYYYEVIDLELGMQPQTILAYEMNGRPLPIPHGAPLRLRVENQ
                     LGYKMAKWINRIEVVNNFKHIGKGQGGWRDDLLHYYPLDAGL"
     gene            complement(4233..>4821)
                     /locus_tag="VFY96_07350"
     CDS             complement(4233..>4821)
                     /locus_tag="VFY96_07350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX5606312.1"
                     /translation="NELLDAMPVRLISDGTEKLVGLDGEKFVFVEQPVDKAIPNQPAL
                     DWIDGIAANLQRGYVIAIDYGFLNEEIERNVQVRAQHRHLVSPFEQIADADITMSVDW
                     RSIVERAQANGLRVAGFSDQHHFLTGIVSQFGSGDRGQSFLADSRKAKRELQTLLHPE
                     MLGRAFQVLALAKNVGCETAPLAGFKFARDSRIAR"
BASE COUNT         1026 a         1347 c         1280 g         1168 t
ORIGIN      
        1 gttggccatc aaggccggtt tggtctcctc gcggtcgata atcgggaggt ttgtttgcct
       61 tgaagtccgg aacgtcgatt ccagtccact tttccaattc ccaccagaca agtttcttgc
      121 gttcgctcca cggctcacca ttcggcttcg cgcttgcgcg attgtaaatg atacggcgat
      181 cacttggcca actgaagccc cagccatggc cgagataatc cttcggtttc cgcgcgttcg
      241 ccttgttcac tccgtctcga ttgaaaacgc cggagtaaat ccagcaaccg caggcggtag
      301 atccatcagg tttcaattcg ttgtaatcgg ccacttgcgg accgcgatgg gcattgcctt
      361 gtcgatcgac gccaccaaag agaatgccat cggtaccacc ggcgtcagcg cgaccggcta
      421 caggttgcgt gttccagcca ttgatctcag caagcactgc ttctattttc ggctcgccta
      481 gttcgtcctg cggataccac caatcgagcg cccgcagcgg ttcgtctatc ggatcagtgg
      541 aggcccttgc tttagcgatt aacctcaggg ccagttgatg aatgaaccat gcgtcggacc
      601 gagaatcacc cggtggatcg actgctttct cgcgccattg caaaagacgc tgcgtgttcg
      661 tgaacgcgcc ttccttttcc gcgtgcccgg ccgcaggaaa gaagaaaacc tccgtctcta
      721 tctcttcagt tctcaattcg ccccgctcga tttcggccga atcgtgccaa aatctggccg
      781 gctcggtttc caccatgtcg cgaatgacga accattttaa tttcgccatg gactttcgtt
      841 gaaagcgcga gtttggcgct ccgacggcgg aattctgtcc aatcagaaac atgccctcca
      901 cttttccgtc cgccatttcg tagaggtatt cgaagaaaga atggtcggcg gtgattttcg
      961 gcagccagtt gtaaccgaaa tcgttttcag cggtcgcgct tttgccgtaa taggccttta
     1021 acaagctcac gaaatacgcg ggcgtattcg accagagccc agtcttggtc gtgtttgtgt
     1081 cgagatattt ttgcaatgtc tcctctgctc cgccacgcgg catcgccaga taaccaggca
     1141 aaatgtcata gagcgttggg atatcggtcg agccctggat cgaggcgtga ccgcgcaacg
     1201 ctagaatgcc gccgccaggg cgtccgatat tgcccaatag tagttgcaga atcgaggccg
     1261 tgcggatgat ctgcacccct ttagagtgtt gcgtccagcc gacggcataa caaatcgccg
     1321 ccgttttgtc cggccccgat gccctaacga gtgcgtcggc gactttgtga aacagcgccg
     1381 gtggaatacc gcagactttc tccaccatct ccggcgtgta gcgcgcgaag tggcgttcta
     1441 gtttttggaa cacgcattgt ggatcttgca acgtgaaatc ccgctcggga aagctgaggc
     1501 cttcgccttt atagagccag ctttgcattg agtaattgcg ttctccttca ttccaaccgg
     1561 agaaaaaacc atccgcgctg tcttccggat cttgataatc gtcgcgcaaa atacaggagg
     1621 catttgtgta gttgacgacg tactcacgga agaacagctc gttctcgata atgtgtctga
     1681 cgagtcctcc caggaacgca atatcgctcc cggcacggat tggcacccag atgtcggcca
     1741 gcgcgctggt gcgcgagaaa cgtggatcga cgtgaataat cgtcgcgccg cgctcctttg
     1801 ccttcattac ccagcgaaag cccaccggat gcgcctcagc catcgaggaa ccttcgatca
     1861 aaatgcaatc tgcattcgcc aggtcttgtt gggcggttgt agctccgccc cggccaaagg
     1921 aggtgcccag accgggcacc gtgctgctat gtcatatgcg agcctggttg gacacacaga
     1981 ccatgcccag gcctccggtg aaaagctttt tgatgagata attttcttcg ttgtcgagag
     2041 tggcaccgcc caggtggcaa atcgaagtgg tgtgattgat tgccgatccg ttttcttgcc
     2101 tgacaaacgt gcgcttgcgc gattcccaaa cacgctcggc gaccatgtcc atcgcccgct
     2161 ggagtggaat ctccatccag tctttcgagc ggggcgcgcg gtattttact ttcgtttggc
     2221 gccgcgaatg cgtcagcagt tggtaggagg cggcgccttt cgggcaaaga ttcccctggc
     2281 tgatcggcga ttccgggtcg ccttcgattg acactagttt gccgtccttg tggaaaacca
     2341 attgaccgca cccgacaccg caatagggac aaatcgagcg cgctacctca gcatcatcaa
     2401 tgcgcgcatg catcgcgcgc gtcttgtctg acatcgcctc gagaccggtg ccgtccttac
     2461 gttcgcggat ctgccgaatc agcggccact ttgagaggaa tgaagacatc aagaagtcat
     2521 cgcgctggta atcacggttg gatgttcgaa ggcagtcagg gaattcacct ggcgtccgtt
     2581 acatccatga tatcaacgcc gcaaaagaga cacaatacgg gaaatccgtt gatcatgagc
     2641 gccctactcc cgtaagcgcc atccggcgga gacaggattt gcgataaata caaaatcata
     2701 cgccgacgat tcgcgttatg gacgatacgc tccaattcgg atttccgttg tgggtccgat
     2761 taacccactt cttcaacttt ttctttatta cgttacttat tcgcagcgga atcgagataa
     2821 tcggtgcgca tccgaagttt tattggcgcg acgactcttt gccaggaagt gaatggttcc
     2881 gcctcacgaa gaaacgactg cccaagaatc agctctggac ggcagaggat gaagtcgagc
     2941 cgatgtctcg atggactgct ttgcctggag aaaacaacct tggcctggga cgtcattggc
     3001 acttttgggc cgcgaacggt tggttgctgg ctggtttgat ttacgtcatt ctgctcttcg
     3061 cgacacctca gtggcgacgc cttctgccaa catcgtggca cgtcttcgcg gcggcctggc
     3121 aggctatcgt ggcctacctc cattttcgac tgcctcctga gggaaacccg ttcaatgcga
     3181 ttcaacaact cacctactgt ttcatcattt tcatcctgtc gccgcttcaa attgttactg
     3241 gaataatgat gtcgccggcg ctgggtgcgc ggttcccctg gtttcccaaa atgttaggcg
     3301 gccggcaagc ggcacgaagt ctccacttca tcgggctgat cgtcttcagt ggtttcatcg
     3361 tcgttcatat cagcttggtg ttcgcacatg gttttggccg tgggatggca aaaatcgttc
     3421 tcggagacga agtctcgtct tatgtgctcg ccacgatgat cgggctattg gcgattgctg
     3481 ccgtgatcgt cttccatatt tggggaacga ggtattcgtt ggtccggccg tcgcaagcaa
     3541 agcgctttct ggaaatcggc attgatcctt tgcgacggct tctttttcgt cattggcatt
     3601 cgcggcaaga gtacctgcgc gtttctgcgt attcgcgtat caatggaaaa ccgccgcgca
     3661 acgaaatgta tgagcgcctg gccgcggcca atttcatgga ctggcggctc gaagtaggtg
     3721 gtttagtaga aaaaaattta agtctctcgc tcaatgacct acatcaaatg ccgcgccaga
     3781 cacaaaccac gttgcactgc tgcatccaag gctggagcta cttcgcgcag tgggccggcg
     3841 tctcggtttc ttcgatcatc gatatgtgcg ggccgctgcc gaatgcgcgg tttctcgttt
     3901 tccactcttt ggatgagaaa tgggagagac caggtcacgg ctattactac gaggtaatcg
     3961 acttggaact cggcatgcag ccgcagacaa ttttggcgta tgaaatgaac ggccgaccgc
     4021 taccgattcc tcacggagcg ccgctgcgat tgcgtgtgga aaatcaactg gggtacaaaa
     4081 tggccaaatg gattaaccgc atcgaggtcg tgaacaactt taagcacatc ggcaagggac
     4141 agggcgggtg gcgcgatgat ttactgcatt attatccgtt ggatgccggg ctttgagcag
     4201 tcgcggcggt tagcagcggg aatcggtgac tcctagcgcg caatgcgcga gtctcgcgcg
     4261 aatttaaaac ccgccagcgg agccgtttcg caacccacat ttttcgcaag tgcgagtacc
     4321 tgaaaggcac gacccagcat ttccggatga agcagcgttt gcaattcgcg tttagccttg
     4381 cgcgaatcgg ccaggaacga ttggccccgg tcgccgcttc cgaactggga gacgattccc
     4441 gtcagaaaat gatgttgatc ggaaaatcct gcgacgcgca acccgtttgc ctgcgcgcgc
     4501 tcgacaatac tcctccaatc cacagacatc gtgatgtccg catctgcgat ctgttcgaat
     4561 ggcgaaacga gatgtcgatg ttgagcccga acctgaacgt ttctctcaat ctcctcgttc
     4621 aaaaatccgt agtcgatcgc aatcacataa ccacgctgta gattcgctgc gattccgtcg
     4681 atccaatcaa gcgcgggttg attaggtatc gctttatcaa ccggctgctc gacgaataca
     4741 aacttctcgc catcgagacc gaccaatttc tccgttccat cactgatcaa gcgcaccggc
     4801 attgcatcca gcagttcgtt g
//
LOCUS       DASULZ010000357         3388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_653538, whole genome shotgun sequence.
ACCESSION   DASULZ010000357 DASULZ010000000
VERSION     DASULZ010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653538"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            498..1370
                     /locus_tag="VFY96_07355"
     CDS             498..1370
                     /locus_tag="VFY96_07355"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606313.1"
                     /translation="MSVWFAKEGNYYEKNGLNVEVISIPGSSLAIQAMLSGELPIIQA
                     GGAGPIQAALSGTDTVIVATIAKKFNWWIFSQPGINRIEDLRGKIFGTTRFGTQSDLA
                     SRIALRRAGIDPERDITMVQTGGPAETISAMVAGKVHAAAVTPPATLQARKAKLKDLV
                     DLSKLDVEYHVNGVVTTRKFLKSNEETVRRFLRAYIEGAVRGMNDKAFAMKSMGKYFR
                     TNDQEVLEETYEMVIKNGFNVPPYPAGIQSLLLELEKTVPKAKTAKPEDFADSRLVKE
                     LDQSGFIKSLMAVR"
     gene            1383..2291
                     /locus_tag="VFY96_07360"
     CDS             1383..2291
                     /locus_tag="VFY96_07360"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5606314.1"
                     /translation="MSEAEDIFRVTLLGTGAPPPSLERFGPSTLVEVGREKFIFDAGR
                     GAMQRLHQLSIPFSEITGIFLTHHHSDHVVGFPDLWLTGWIGRPWGKRRAPLPVWGPA
                     GTKEMMEHLPKAFHVDIRVRSKSYPPDGVTLVANEIIEGSILDKDGITISAFKVDHGG
                     EDLPAFGYRIDYRGRAAVLSGDTTFNENLIERARGADLLVHEVTAVAGSAAENPEQLK
                     RISSNHTTPQQAGEIFARVEPKLAVYNHLLLFGHATAKDLIPATRQKYAGPLIVGEDL
                     LAIAIADEVQAKAFGSSIKNYTRGRL"
     gene            2288..3292
                     /locus_tag="VFY96_07365"
     CDS             2288..3292
                     /locus_tag="VFY96_07365"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606315.1"
                     /translation="MKYQKTGVAMVVCYALVLLNSVCFAAASPEALTVGYSSFSGAYL
                     PLWIAVEEGLGKKYGLDLKAIYAGRIRPQQLLATGEVPFVMATGTGALTSHILGVKDQ
                     VIIMTTINRVNSAIFSRPDIKSAEELRGKTVATGRPGAFGDTMVRYVLRAKLGLMPDR
                     DVKMLPIGEAQLAFPALERGVVQAASLTIPFTLIAKKMGYRELIDYDKAGVVYPYNTI
                     TTLRQTPSKNPDLADRLLKALIEGIFVSKTEKEKSLAVLKRYMKGTPDDILEETYQYS
                     KNVAEEVPTPSLEIVKTALEILSYQYPQAKGTDPNPIIDTSIMKRIEQSGFLKTIYKK
                     "
BASE COUNT          815 a          882 c          955 g          736 t
ORIGIN      
        1 acgcacttat cccagttttc tacatcacgg ttccctcgat aggcgcttcg cgctactcgg
       61 gaaatcggaa cgaaccgaat ctcctgagta ggcagcgagc agcgctgccg tagcgaagga
      121 tatgcgctcc ctgctctgcg agaaatccaa tcgttctgag agcgtagcaa agaatagcgc
      181 gcttttgcgc tacttggcca atgccgacgc tgctgaacga aacgacagtt ggactctgac
      241 taaagcgcgg aattttccca tccggcattt gtgccaaacc ggtaaagcga ttacggtaaa
      301 tcgaaattca tcgatttatt gtgatatatg aagtcgcaac gcgtgttgag attctgaagc
      361 agagaggggt ttacatggtt tgtcggatcg tgattctttt tatattcgtc gcaagcactt
      421 tttcgatacc ggcattctgc caagagaaac cggtgcaaaa attccgcacc ggcggggggt
      481 ctgcctccgc ggcacagatg tctgtctggt ttgctaaaga aggaaattat tatgagaaga
      541 acggcctgaa tgtcgaggtg atcagcattc ctggcagctc gctggcgatt caagcgatgc
      601 tctcgggcga attgccgatc atccaggccg gcggcgccgg accgattcaa gcggcgctct
      661 cgggcacgga cactgtgatc gtcgcgacca tcgcgaaaaa attcaattgg tggattttca
      721 gccagcccgg catcaatcga attgaagatt tgcgtgggaa aatcttcggc acgacgcgtt
      781 ttggcacgca gtctgatctc gcgtcgcgca tcgcgctgcg gcgcgcgggg atcgatccag
      841 agcgcgatat cacgatggtc cagacgggcg gaccggcgga aacgatcagt gccatggttg
      901 cgggcaaggt tcatgcggcg gcggtcacgc cgccagcgac tttgcaggcc cgtaaagcca
      961 agttgaaaga tctggtcgat ttatcgaagc tcgacgtcga atatcacgtc aatggcgtgg
     1021 tgacgacgcg caaatttctg aagtctaacg aagagaccgt gcgacgattt ctgcgtgcct
     1081 acattgaggg cgcggtgcgc ggcatgaacg acaaagcgtt tgcgatgaag agcatgggaa
     1141 aatatttccg caccaatgac caagaagtgc tcgaagagac ctacgagatg gtcatcaaga
     1201 acggtttcaa cgtgccacct tatccggcgg gcattcagag cctgttgctg gaactcgaga
     1261 aaacagttcc caaagcgaaa accgccaagc cggaagattt cgccgacagc cgtctggtca
     1321 aagagctgga ccaaagcggt tttatcaagt cattgatggc tgttcggtga aaggaactcc
     1381 ccatgtccga ggcagaagac attttccggg taactcttct cggtaccggc gcgccgccgc
     1441 caagtcttga gcgttttggg ccaagcacac tcgtcgaagt agggcgcgaa aaatttattt
     1501 tcgatgccgg gcgtggcgcg atgcagcggc tgcaccagtt gagcatccca ttcagcgaaa
     1561 taaccggtat tttcctgact catcatcact ccgatcacgt cgtcggcttt cccgatcttt
     1621 ggctcaccgg atggatcggc cgcccatggg gcaagcggcg tgcgccgcta ccggtctggg
     1681 ggccagcggg caccaaagaa atgatggagc atttgcccaa ggcttttcac gtcgacatcc
     1741 gtgtgcgcag caaaagttat ccgcctgacg gagtgacttt ggttgccaac gagatcatcg
     1801 agggcagtat tctcgacaag gacggcatca cgatcagcgc gttcaaggtc gatcatggcg
     1861 gcgaggattt accggcattc ggttatcgaa tcgattatcg cggccgcgcg gcggtattgt
     1921 ccggggacac aactttcaac gaaaacctga tcgaacgtgc gcgtggggcg gatcttttgg
     1981 tacacgaggt cactgcggtt gcaggaagtg cggctgaaaa cccagagcag ctaaaacgga
     2041 tcagctccaa ccacacgacg ccgcagcagg ccggcgaaat ctttgcccga gttgagccta
     2101 agctcgccgt ttacaatcat ctacttttgt tcggccatgc taccgccaag gacttgatcc
     2161 ccgcgactcg gcagaaatat gcgggtccgc tgatcgtcgg cgaagatctt ctagcgatcg
     2221 cgattgccga tgaagtacag gcgaaggcat tcggaagtag cattaagaat tatacgaggg
     2281 gtcgcctatg aaataccaga aaacaggggt tgcgatggtg gtctgctacg ccttggtctt
     2341 gctcaactct gtctgcttcg cggcagcttc tccagaagcg ctcacggtcg gctactcgtc
     2401 tttctccggc gcctatttgc cgctttggat cgcggtcgaa gaaggcttgg ggaaaaaata
     2461 cggtctcgat ctcaaagcga tctatgccgg gagaatacga cctcagcaat tgcttgcgac
     2521 cggcgaagtg ccatttgtca tggccaccgg aaccggcgcg ctcacttcgc atattctcgg
     2581 tgtcaaggat caagtgatta tcatgaccac catcaacagg gtcaattcgg cgattttcag
     2641 ccgtcccgat atcaaaagcg ccgaggagct gcgcggcaaa accgtggcga ccgggcgtcc
     2701 cggtgcattc ggcgatacca tggtgcgtta cgtgctgcgc gcaaagctcg gtttgatgcc
     2761 ggatcgcgac gtgaaaatgc tgcccatcgg cgaagctcaa ttggcatttc ccgcgttgga
     2821 gcgtggcgtt gtccaggccg cttccttgac gattccgttc acgctgatcg ccaagaagat
     2881 gggttaccgc gagctcatcg attacgacaa ggccggtgtc gtctatccat acaatacgat
     2941 tacgacgctg cggcaaacgc cgagcaaaaa tcctgatctg gcggaccgac tcttgaaggc
     3001 gctgatcgag gggatcttcg tctcgaagac cgaaaaagag aagagcctgg ccgttctaaa
     3061 gaggtatatg aaaggtactc ccgacgatat tctggaagaa acctatcaat attcaaaaaa
     3121 tgtcgcagaa gaagtgccga cgccgtcctt ggaaatcgtc aaaaccgccc tggagattct
     3181 ttcgtaccag tatccccaag ccaaagggac ggatccgaac ccgatcatcg acacgtcaat
     3241 tatgaaacgg attgagcaga gcggatttct gaaaacgatc tacaagaaat agcattcttt
     3301 ccaaagcgag atcttggcgc tagattctag gtgagacatc cggtctattc gcggatgtta
     3361 gatcacaagg agggattaat tatagcga
//
LOCUS       DASULZ010000358         5017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1364381, whole genome shotgun sequence.
ACCESSION   DASULZ010000358 DASULZ010000000
VERSION     DASULZ010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364381"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VFY96_07370"
     CDS             complement(<1..233)
                     /locus_tag="VFY96_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007260250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdenumtransferase MoeA"
                     /protein_id="HEX5606316.1"
                     /translation="MAQTFFKVLAPREALNVLLTANPVGTEQIPSVHARARVLADELR
                     SAVDLPHFHRAAMDGYAVIAKDTFGASQSLPAY"
     gene            complement(357..1370)
                     /locus_tag="VFY96_07375"
     CDS             complement(357..1370)
                     /locus_tag="VFY96_07375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX5606317.1"
                     /translation="MKFDASLLVHQLEQMPALARFADEAGFDGIWTFETAHEAFLPLV
                     LAAEHSSRLSLGTSIAVAFPRSPTILAQIAWDLARYSKGRFILGLGTQVKGHNERRFG
                     VKWEKPVEKMREVVLAIRAIWDCWQNRTPLDFQGEFFKLTLMTPFFSPEPHEYFRIPI
                     FVAGVNRRMCQLAGELCEGFHAHPLHSVRYLRERVLPNIEVGLKQSGRERTSIELSSS
                     IFVVPTDDPSATAYEAEIREQVSFYASTPPYRPVFELEGWGEIADRLKGLAARGEWSA
                     MPRLISDEMLERFAVRGTWAELPDKVLKKYDGLLDRVSYYFPLVPGANEAKWRATVTG
                     FKR"
     gene            1587..2573
                     /locus_tag="VFY96_07380"
     CDS             1587..2573
                     /locus_tag="VFY96_07380"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606318.1"
                     /translation="MAVHINKPAIALALMILVMFWANLLRAQEKPSAEKIRIAIATSS
                     MAFLVPFVARDRGLYAKYGSEVELIQMRPNISVAALLSGEIEYIELIGSAIRSAARGM
                     PMRAISTGIKSPFFSVVAQNKYKTVKDLRGAIIGITSIGGTNHVSTRITLRQFGLDPE
                     KDVKLLAIGDEKIMYDAFKAGRADAVVLAPPFSVQLKREGFPILANTAQYVTIPFSGL
                     GTTTDRIKNNRAQIKRVLKAEIEALRFIQSNAAGTTEVIRKRFNMDEKLARESYDVVV
                     EAFSRDGRVPLDGVDILLQIEKDQKLIPTTVTPQMVVDSSLIDEALKEMSGK"
     gene            complement(2600..4195)
                     /locus_tag="VFY96_07385"
     CDS             complement(2600..4195)
                     /locus_tag="VFY96_07385"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX5606319.1"
                     /translation="MGYFKDVREHIAALDQAGLLVKIDEQINKDTELMPLVRWQFRGL
                     SEEQRRAFMFTNVVDAKGRKYDIPVVVGTLAASRQIYGFGLQCKPEEIVDRWNQAQRQ
                     PIPPKTVDDAPVHEIVYQGSSLMEKGGLGEFPIPISTPGYDCAPFTTASHWFTKDPET
                     GIINIGNYRGHVKSPTRTGVFLSSSNHGGYHWRKCQERGIPLQAALVIGAPPCVAYTA
                     PSRIPFTVSELDVAGGLAGAPIEVVKCKTSDVLVPAHAEIVIEGEISTEFLEPEAPFG
                     ESTGYIAERLLNPFCEVKCITHRKNPILATIISQMPPSESSVMRQVAAEGNYLRFLKD
                     QCNIPGILEVAFHSIAVRQICVIKMKKINQAHVWQALHSMVGYNPATGKMVIAVDDDI
                     DPRNLDSLFWAMAFRMQPHRDVLIIRNRTPQMDPSVFPPGSEGGHSLDEIAGASAMLI
                     DATRKWDYPPVSLPKKEYMERARQLWERLELPKLKPQDPWFGYNLGLWSEEDEAEAEL
                     AVKGDHYQTGEKRLQQRVPVKKL"
     gene            complement(4507..>5017)
                     /locus_tag="VFY96_07390"
     CDS             complement(4507..>5017)
                     /locus_tag="VFY96_07390"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5606320.1"
                     /translation="LVSLIQSNFMGFGSGVVEPDTAIILQNRGHLFSLEETHPNCIGP
                     RKRCVHTLMPGLVVKDGRPCGVLGLKGGHVQPQVQVQILSKLIDFGMTPQEAISAPRF
                     NHIESAKVGLEPEIAQSVKDDLTKRGHQIVAGNPESFGGAHAIFVHPESGAFVGGSDP
                     RKGGCALGF"
BASE COUNT         1101 a         1508 c         1351 g         1057 t
ORIGIN      
        1 aaataagccg gtaaactttg actcgcgccg aaagtgtctt tcgcgatcac ggcgtagccg
       61 tccattgccg cgcgatggaa gtgcggcaga tccaccgctg agcgaagctc atcggcaaga
      121 acgcgcgcgc gtgcgtgcac cgaaggaatc tgctccgtcc cgacagggtt cgccgttaga
      181 agaacgttca gcgcctcgcg cggcgccaac actttgaaga acgtctgcgc catttatggg
      241 caaatcatag ccagaaccaa tgccgcgaat caaaccaccg attccagtac ggtttcggtt
      301 gaaagactcg cctaacaccg aacgaaagcg gggcggaact cacaatgcga gagtcttcac
      361 cgtttgaatc ccgtaaccgt cgcgcgccat ttggcttcat tcgcgccagg cacgagcggg
      421 aagtagtaac tgacgcgatc caataggccg tcgtattttt ttaaaacctt gtccggcaac
      481 tcggcccacg tgccacgcac ggcaaatcgt tcgagcatct cgtcgctgat cagcctcggc
      541 atcgcgctcc attcgccccg cgccgctaat cccttgagcc gatctgcgat ctcgccccag
      601 ccttctagct cgaacactgg tcggtagggt ggcgtgctgg cgtaaaaaga aacctgctca
      661 cggatttccg cttcgtacgc cgtcgcgctt ggatcgtccg taggaacgac gaaaatactg
      721 ctcgacagct cgatggatgt tcgctctcgt ccgctttgtt tcagaccgac ttcgatgttc
      781 ggcagcacgc gctcgcgcag ataacggaca ctatgcagcg gatgcgcgtg aaacccctcg
      841 cacagttcac cggcgagctg gcacatgcgc cgattcaccc cggcgacgaa aatcggaata
      901 cgaaagtact cgtgcggctc tggactgaaa aaaggcgtca taagcgtgag cttgaagaac
      961 tcgccttgaa aatcgagcgg cgtgcgattc tgccagcagt cccagatcgc ccggatcgcg
     1021 agaacgacct cacgcatttt ctccaccggc ttttcccact ttacgccgaa gcgccgctcg
     1081 ttgtggcctt tcacttgcgt gccgaggccg agaataaatc tccctttaga ataccgcgca
     1141 agatcccatg caatctgcgc taggatcgtc ggactgcgcg gaaatgccac agcgatgctc
     1201 gtgccgagag acagtcgtga gctatgttcg gcggcgagga ccagcggcaa aaacgcttcg
     1261 tgcgccgttt cgaaggtcca gatgccatcg aatccggcct catcggcgaa ccttgccaat
     1321 gccggcattt gttccagctg atgcacgagc aagctcgcgt cgaatttcat ccgattaagt
     1381 cgttctctaa gctcgatccg aaactattat ctcgccgaat gtttgtgatt ctgcgtcctt
     1441 cggctacttt caggacaagc cgcgaggaat ctttccctat cggtgacggt gcccttgcat
     1501 gctcatggtg cttgattaaa ttgctctacc aactagaatc gctctccaca acataatgcg
     1561 catacgacgg ctccgggagg ttctgggtgg cagtacacat aaataaacct gcaatagctt
     1621 tagcgttgat gattttggtt atgttttggg cgaacctgct ccgagcgcag gaaaagccga
     1681 gcgccgaaaa aatccgcatc gccatcgcca caagctccat ggctttcctc gtgccgttcg
     1741 tggccaggga ccgcggcttg tatgcgaaat acggctccga ggtcgagctg atccagatgc
     1801 gtcctaacat ctcagtggcc gcgttgctca gcggcgagat cgagtacatc gagctcatcg
     1861 gctccgcgat ccgctccgcc gcccgcggca tgccgatgcg ggcgatctcc acgggaatta
     1921 agtcgccgtt cttcagcgtc gtggcccaga acaaatataa aaccgtcaaa gatctcagag
     1981 gcgccattat cggcatcacc tcgatcggcg gcaccaatca cgtgagcacg cgcattacgc
     2041 tccgccagtt cggcctcgat ccggaaaagg acgtgaaact gctggcgatc ggcgatgaga
     2101 agatcatgta cgacgccttc aaagccggcc gcgccgacgc cgtcgtgctg gcgccgcctt
     2161 tttcggtgca gctcaaacgc gaaggcttcc cgatcctcgc taacacggcg caatatgtaa
     2221 ctattccctt ctccggcctc ggcacgacca ccgaccggat caagaacaat cgcgcccaga
     2281 tcaaacgggt cctgaaggcg gaaatcgaag cgcttcgatt catccagagc aacgccgccg
     2341 gaaccactga agtgatccgc aagcgcttca acatggacga aaaactagcg agggagtcct
     2401 acgacgtcgt tgtcgaagct ttcagccgcg acggccgagt gcctctcgat ggtgtcgaca
     2461 ttctgctaca gatcgaaaaa gaccagaaac ttattccaac caccgtcacg cctcagatgg
     2521 tcgtcgattc gtcgctaatc gacgaagcgc tcaaagaaat gagcggcaaa tagtcttaaa
     2581 gcggattacg tcttctcggt cacaacttct ttaccggcac ccgctgttgc aagcgctttt
     2641 caccggtctg gtaatggtcg cctttgaccg caagctcagc ttcggcttca tcttcttccg
     2701 accacaaacc taaattgtag ccgaaccagg gatcctgcgg tttgagcttc ggcaactcca
     2761 gtcgctccca aagctgcctc gcgcgttcca tatattcttt cttcggcagt gaaaccgggg
     2821 ggtaatccca tttccgtgtg gcgtcgatca gcatggccga agcgccggcg atctcgtcga
     2881 gagaatgacc gccttcgctg cctggtggaa aaaccgacgg gtccatctgc ggcgtacggt
     2941 tgcggatgat cagcacgtcg cgatgcggtt gcatgcgaaa agccatcgcc cagaaaagcg
     3001 aatcgagatt gcgcgggtca atgtcgtcgt ccacggcaat caccatcttc ccggtggcgg
     3061 ggttatagcc gaccatactg tgcagcgcct gccagacgtg cgcctggttg atttttttca
     3121 tcttgatgac gcaaatctgg cgcaccgcga tgctgtgaaa ggcgacctcg agaatcccgg
     3181 ggatgttgca ctgatctttc aaaaacctga gatagttccc ttcggccgcc acctggcgca
     3241 tcacgctcga ttcgctcggc ggcatctggc tgatgatcgt cgccagaatc ggattttttc
     3301 tgtgcgtgat gcatttcact tcgcagaacg gattgagcaa tcgctcggca atgtaaccgg
     3361 tggactcgcc gaacggcgcc tcgggctcaa gaaattcggt cgagatctct ccctcaatca
     3421 cgatctcggc atgcgcaggc accagaacat cgctcgtctt gcacttcacg acttcaatcg
     3481 gcgcacccgc gagtccgccg gcgacatcga gctcactgac cgtgaaagga attctggacg
     3541 gcgcggtgta cgccacgcac ggcggcgcgc cgatcaccag cgccgcttgc agtgggatac
     3601 cgcgctcctg acatttgcgc cagtgatagc cgccgtggtt gctcgagctc aagaacacgc
     3661 cggtccgggt cgggcttttc acatggccgc ggtaattgcc gatgttgatg atgccggttt
     3721 ccggatcctt cgtgaaccag tgcgatgcgg tagtaaacgg cgcgcaatca taacccggcg
     3781 tcgaaatcgg aatcggaaat tctcccaagc cgcccttctc catcagcgat gaaccctggt
     3841 acacgatttc atggaccggc gcatcgtcga cagttttcgg cggaatcggc tgacgctgag
     3901 cttgattcca tcgatcgacg atctcttcgg gtttgcattg caggccgaag ccgtaaattt
     3961 gtcgcgatgc ggcgagggta ccgaccacca ccggaatgtc gtacttgcgc cctttggcgt
     4021 cgacgacatt ggtaaacatg aacgcccggc gctgctcctc agagagccca cggaattgcc
     4081 agcgcaccag tggcatgagc tcggtgtcct tgttgatctg ctcgtcgatc ttgaccagca
     4141 gtccagcttg atccagcgcc gcgatgtgct ccctgacatc cttgaagtac cccatgcaaa
     4201 cgctcctcga cccaaaacgt tgtagacggt gatatgcgat ttcaaaaaag aaaaccagcg
     4261 gcagaggcgt tcacaagact tgcgcttccg gtacgtgtca ccctgagcga aagcgaaggg
     4321 tctactatcg gcaacgagat tcttcgtcgc tacgctcctc agaatgacac atggagagag
     4381 agcggcgatc gctaacccgt tgacacaaag cgaatccgag ttaccgcaat gacccgaatc
     4441 tcgtttcact ccatacagac ttcagttgga atgttttgaa cgattcgaac ggccggaact
     4501 aatcctttaa aatcccaggg cgcagccgcc tttgcgcgga tcggagcccc cgacgaaagc
     4561 gccggattcc ggatggacaa agatcgcgtg cgcgccgccg aagctctccg gattgccggc
     4621 gacgatctga tgaccgcgct tggtcaaatc gtctttgacc gattgcgcga tttccggctc
     4681 gaggccgact ttggcactct cgatatggtt gaacctcggc gccgagatcg cttcctgcgg
     4741 cgtcatgccg aaatcgatca gcttggacag aatctgtact tgcacctgcg gctgcacgtg
     4801 gccgcccttc aaacccagaa ctccgcatgg cctgccgtct ttgacaacca agcccggcat
     4861 gagcgtgtgc acgcagcgct tgcgcgggcc gatgcaattc ggatgagttt cctcgagcga
     4921 aaaaaggtgt ccgcggttct gtaaaataat cgcagtgtcg ggctcgacaa cgccggaacc
     4981 gaatcccatg aagttgcttt gaatcaacga aaccagg
//
LOCUS       DASULZ010000359         2435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_348674, whole genome shotgun sequence.
ACCESSION   DASULZ010000359 DASULZ010000000
VERSION     DASULZ010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348674"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VFY96_07395"
     CDS             complement(<1..651)
                     /locus_tag="VFY96_07395"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5606321.1"
                     /translation="MYNVKGFIDADGHVVESDSELLQFLPKPFRNREDLLSFPFFPTL
                     DGFHRQARRVADGQGRYIAKGSLKQWQEFLDDDQIAWSVLYPTAGLTFGLIRDTDWAC
                     AIAKGYNNYLYETFTKPEKRLKGMALIPLQSIPDAIEELRRAVTDLGFVGAVLPAVGL
                     RQPFGDPKYFPVYEAAQELDALLAVHAAPTQGIGVDGFDKLIEVRTLSHGFGQMIQM"
     gene            complement(898..2418)
                     /locus_tag="VFY96_07400"
     CDS             complement(898..2418)
                     /locus_tag="VFY96_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015213114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5606322.1"
                     /translation="MVTLTLEHLFLVAVSTGIAILIGVPLGILLTRKPALSKPVLGFA
                     NIMQTVPSLALFGFLIPLNIYLFHVKIFGGIGARTAIVALVLYALLPIIRNTFTGIRS
                     VDPAIRDAGRGMGMTDRQLLFQVELPLALGVIIAGVRVATVICVGTATIAAAIDAGGL
                     GRYIFRGLRANDNVLILAGAVPAALIAIAADLALGYFERTVQLGQRTRVSGKKIIIAS
                     TGVLCAAIVIVYFAVGGSSARIAVGSKDFTEQVIVGEILAQTIENKTRLQVTRRFDLG
                     GNLAHEALVAGEIDTYVEYTGTALLAILKSRPITDPAEVLRRVKQDYAERFQLEWTEP
                     LGFNNTFAVLVRGEDANKFKLKTISDAAKISPQWRAGFGQDFMSRADGYPGFARTYGF
                     HFAEIREMDLSLTYRALAEKQVDLIAGNSTDGLISRYGLVQLVDDRHYFPPYDAVPVI
                     RRAILERHPEIRDALRQLGGILSVVEMRKLNYAVDGEKRPAREVAREFLETKQILR"
BASE COUNT          577 a          715 c          626 g          517 t
ORIGIN      
        1 catttgaatc atctggccaa agccgtgact caaagtgcga acttcgatca gcttgtcgaa
       61 gccatcgacg ccgatacctt gcgtcggcgc tgcgtgaacg gcgagtagcg cgtcgagctc
      121 ctgcgctgct tcgtaaaccg ggaaatactt cgggtcgcca aacggttgcc gcaatccgac
      181 cgccggcaga acggcgccga cgaagcctaa atccgtcact gcgcgccgga gttcctcgat
      241 tgcgtccgga atcgactgca gcggaatgag cgccatccct ttcagacgtt tttccggctt
      301 ggtgaaggtt tcgtagagat aattgttgta tcccttagca atcgcacagg cccaatccgt
      361 atccctgatc aggccgaagg tgaggccagc ggtgggatat aaaaccgacc atgcaatctg
      421 gtcgtcgtcg agaaactctt gccactgttt tagactgccc ttggcgatat aacggccttg
      481 cccgtccgca accctgcgcg cctgccgatg aaagccatcg agcgtcggaa agaacgggaa
      541 cgacaggaga tcttcacgat ttcgaaaggg tttcggtaaa aactgcaaca actccgaatc
      601 gctctcgacc acatgcccat cggcatcgat gaatcccttt acattgtaca tatttatccc
      661 ttgttattaa cgtaattctt ggcgggttaa agttttggct cgtccgtgag ttaaaaactt
      721 ttgtacatcg atcgaattcg ttgtgcaatt ttccgcaggc ctcaacccgg gctaaatccc
      781 cgcacgcacg ataactgaac tcggcgtcca ctccgctttc catcgtcaac gaccgtgcgc
      841 tatcaatttg ggcccacgcc gaggaaaccg cggccaggaa attttcctgc tcatgtctta
      901 ccgcaagatt tgtttggttt ccaggaactc gcgtgctact tcccgtgccg gtcgcttctc
      961 gccatccacg gcatagttga gtttgcgcat ttcgacaacg ctcagaatgc cgccaagctg
     1021 acggagcgca tcgcgaattt ccggatgccg ttccaaaatc gcccgccgaa ttaccggcac
     1081 ggcatcgtag ggcggaaaat aatgtcgatc atccaccaac tggaccaggc cgtagcggga
     1141 aatcaaacca tccgtcgaat taccggcaat taaatccacc tgtttctccg ccaacgcccg
     1201 ataagttaac gacaggtcca tctcgcggat ttctgcaaaa tgaaatccat acgtccgagc
     1261 gaagccggga taaccatcgg ctcgcgacat gaagtcctga ccgaaaccgg ctcgccattg
     1321 cggcgaaatc ttcgcggcat cactgatggt tttcaacttg aacttattgg catcttcacc
     1381 gcgcaccagc acggcgaaag tgttgttgaa accgagcggt tcggtccact ccaattgaaa
     1441 ccgttcggca tagtcttgct tgacgcgacg taaaacttcc gctggatcgg tgatcggccg
     1501 gctcttcaat atcgccagga gcgcagtccc tgtatactcc acgtaagtat caatctctcc
     1561 ggcgaccagg gcttcgtgcg cgaggttgcc acccaaatca aaacgacgcg ttacttgtag
     1621 cctggttttg ttttcgatcg tttgcgcaag aatctcgccg acaatcacct gctcggtaaa
     1681 atccttggaa ccgaccgcga ttcgagcaga gctccctccg acggcaaaat acacaatgac
     1741 aatcgcggcg cataatacgc cggtgctggc gatgataatt ttttttcccg aaacgcgagt
     1801 ccgttggccc agctgtaccg tcctctcgaa ataccccagc gccagatccg cggcgatcgc
     1861 gatcaaagcc gccgggactg cgccagcgag aatcagaacg ttgtcatttg ctcgcaatcc
     1921 gcgaaaaatg tatcggccta aaccgccggc atcgatcgct gccgcgatgg tcgccgtgcc
     1981 aacgcagatc accgtcgcta ctcgcacacc ggcgataatc actccgagcg cgagaggcaa
     2041 ctcgacctga aaaagcagct ggcggtcggt cattcccatg ccgcgaccgg cgtcgcgaat
     2101 cgccgggtcc acagagcgaa tgccggtaaa ggtgtttcgt atgatcggca ggagcgcgta
     2161 aagcaccagc gcgacaatgg cggtgcgcgc gccgatgccg ccgaagattt ttacatgaaa
     2221 taagtaaata ttcagaggaa tgagaaagcc aaaaagcgcc aggctcggca ccgtctgcat
     2281 gatattggca aagcccagaa ccggcttgct cagcgccggc tttcgagtca gcagaatgcc
     2341 gaggggcacg ccgatcaaga tcgcaatgcc ggtcgacacg gcaactagaa aaaggtgttc
     2401 gagagtcagc gtgaccaatt cgccgacatt gcgtg
//
LOCUS       DASULZ010000360         3987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1262443, whole genome shotgun sequence.
ACCESSION   DASULZ010000360 DASULZ010000000
VERSION     DASULZ010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262443"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="VFY96_07405"
     CDS             complement(<1..983)
                     /locus_tag="VFY96_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606323.1"
                     /translation="MRNLIDRRTFLKTSAALAVSAAAGRYLHENAYGASRDRVTIYHS
                     SVADSIHPYNHSSSPIYGNWQHVMEPLVELDYKKQDYVGILADSWQFQGNKWTFKLKK
                     NIKFHNGAPLTSKDVAFSIEKMRDEKGGSLQAPNFKDVTEVQTPDDLTVVFVTKQPLA
                     IFLDRLENRFILSKVAGDKFGDKLYENPIGTGPYKFVSYQRGGNMVFTRNDEYWGGKA
                     AIKEIVFRKVTEDAARLAALESGQADFINNVPDHEVARLQKHPRIRVDKIEGLRMFFL
                     AFNTSFKPWDNKLVRQAANYSVDAPAIVKNIFDGIGYPINGPVGANVIGADP"
     gene            complement(1057..1896)
                     /locus_tag="VFY96_07410"
     CDS             complement(1057..1896)
                     /locus_tag="VFY96_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009297088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5606324.1"
                     /translation="MLSLRYLKVWIGGAIVLLLCVCGVAAPLLAPRDPQAQTLEDRLR
                     PPRWNENGSAAYFLGTDNLGRDMLSRIIYGSRISLIVGAATVLFAGLVGCTLGAIAGY
                     FGRGADEVVSKISEIFLAFPFLLIAIAIMAFLGQGVGNLIMALMLSRWVQYCRVVRGE
                     VLSLKERDFITAAKALGGKDWYVLLKHVVPNTFASVTVIATFGMAIVIITEASLSFLG
                     LGVPVHIPTWGSMLSEGRSYINRAPWLTIFPGLAIFITVFGINLLGDGLRDIFDPKLR
                     RQR"
     gene            complement(1896..2894)
                     /locus_tag="VFY96_07415"
     CDS             complement(1896..2894)
                     /locus_tag="VFY96_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5606325.1"
                     /translation="MNTLGRRLIGAIPVLLGISFLIFLLMHIAPGDPVTLLLGDDATP
                     ADIERTRHEWGLDQPLIVQYYEFLNRAVRGDFGRSLKFNESVTKLIAERLPATLELAF
                     ASLCVAIIIAVPLGVYSAIKHDSLFDHAGMSIALIGVSLPNFWLGIMLIYFLGGQLNL
                     LPVAGRIEYGVDVKTVTGLYLLDSLLTANFAAFWSVLQHLLMPAFTLGSALAAIVTRI
                     SRSSVLEVMRQDYVTTARAKGLSETNVIWRHILRNALITIVTILGLQLGALLSGSVIT
                     ETVFSYPGIGDLLIQSISARDYKLTQVLILMFAITYFVVNLLVDLLYSWIDPRIKF"
     gene            complement(2972..>3987)
                     /locus_tag="VFY96_07420"
     CDS             complement(2972..>3987)
                     /locus_tag="VFY96_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX5606326.1"
                     /translation="RLARITPGRLEKSFFTNSGTEANETAVLLAQLHTKCQDVITLRH
                     AYSGRSYLALSLSAQSSWRLTPNVVPGVHHIANAYCYRCPFGLTYPSCDLKCAKDAEE
                     AIQTMTSQGRVAAFLAEPIQGVGGFIVPPKEYFKEIVGIIRKYGGLFISDEVQTGWGR
                     TGGKMFGIEHWGVDPDIMTFAKGMANGVPIGATIATPEVADSMKGNTISTFGGNPVTC
                     TAASATIQVIEENNLVENARLMGNRLREGLNGLKEKYPVIGDVRGMGLMQGMELVGEK
                     KLPDAESAKRVMELTKRNGLLVGKGGTYGNVLRVAPALNVNKDQIDHALSVLDQSFSQ
                     LGQ"
BASE COUNT          958 a         1136 c         1047 g          846 t
ORIGIN      
        1 ggatcggcgc cgatgacgtt ggcgcccacc ggaccgttga tcggataacc gataccgtcg
       61 aaaatattct tgacgatcgc cggcgcgtct acagagtaat tcgctgcctg ccgaacgagc
      121 ttattgtccc acggtttgaa agacgtattg aaggcgagaa aaaacattct caggccttcg
      181 attttgtcga ctcgaatgcg tgggtgcttc tgtaatcggg ccacctcatg atccggcacg
      241 ttattaataa aatccgcctg cccggattcc agcgcggcta gtcgcgccgc atcttcggtg
      301 actttgcgaa agacaatttc tttgatcgcg gccttgccgc cccagtactc gtcattgcgc
      361 gtgaacacca tattgccgcc gcgctgatag ctgacaaact tataaggtcc ggtgccgatc
      421 gggttctcgt agagtttgtc gccaaactta tcgccggcaa ctttgctcaa gataaagcgg
      481 ttctccaaac gatcgagaaa aatcgccagc ggctgtttgg tgacgaacac aaccgtcaaa
      541 tcgtcgggcg tttgcacttc ggtgacatcc ttaaaattcg gcgcctgtaa gctcccgcct
      601 ttttcgtcgc gcatcttctc gattgaaaac gccacatctt tcgaggtgag cggtgcgcca
      661 ttatgaaact tgatattctt cttgagtttg aacgtccatt tgttgccctg gaactgccac
      721 gaatcggcga gaatgccgac gtaatcttgt ttcttgtaat cgagctcgac gagcggctcc
      781 atcacatgtt gccaattgcc gtagatcgga ctcgagctgt gattgtaggg atgaatggaa
      841 tcggcaacgc tcgagtggta gatcgtcact cgatcgcgcg acgctccata ggcattctca
      901 tgaagatacc gacccgcggc tgcgctcacc gccaaggccg cacttgtttt caaaaaagtt
      961 cgccgatcga taagatttcg catcttcaca tctcctattt cgtccgtgcc cggtgacggt
     1021 tctttgccgg gaagccaaat gccattattt ttatggttac ctctgcctcc ggagcttggg
     1081 atcaaaaatg tcgcgcagcc catcgcccag caagttgatg ccgaacacgg taataaaaat
     1141 cgccaggccg gggaaaatcg tcaaccacgg cgcgcgattg atgtagcttc ggccttcgct
     1201 gagcatcgag ccccaggtcg ggatatgcac cggcacaccc aggccgagaa agctcaagct
     1261 cgcctccgtg atgatcacga tcgccatgcc gaaggtcgca atcacagtga cgctcgcaaa
     1321 agtattcggt acaacatgct ttagcagcac ataccaatcc ttgccaccga gggctttggc
     1381 tgcggtgata aaatcgcgct ccttgagcga aagaacttcg ccgcgtacaa ctcgacaata
     1441 ctgcacccaa cggctaagca tcagggccat aatcagattg ccgaccccct ggccgagaaa
     1501 agccatgatc gcaattgcta tcagcaaaaa agggaatgct aaaaaaattt ctgagatttt
     1561 gcttaccact tcgtcagcgc cgcggccgaa ataaccggca atagcgccga gcgtgcaacc
     1621 gaccaggccg gcaaaaagca ccgtagcggc gccgacgatc aaagatattc gtgatccata
     1681 aataattcga ctgagcatgt cgcggccgag attgtcggtg ccgagaaagt aagcggccga
     1741 accgttctca ttccaacgcg gcgggcgcag tcggtcttcg agggtttgcg cctgaggatc
     1801 gcgcggcgca agcagcggag cggcaacacc gcaaacgcag agcagcaaca caatggcccc
     1861 gccgatccac actttaaggt agcgtagcga taacattaaa acttgatcct cggatcgatc
     1921 cagctgtaga gcagatcgac cagcagattg accacgaaat aggtgatggc aaacatcaaa
     1981 atcaatactt gcgtgagttt gtagtcgcgc gccgatatgg actggatcag taaatcgccg
     2041 ataccaggat acgaaaagac ggtttccgta atcactgaac cactcagcaa cgcgcccaac
     2101 tgaaggccca ggatcgtcac gattgtgatc agcgcgttgc gcagaatatg gcgccagatg
     2161 acgttggtct cactcaggcc ctttgctcgt gccgtggtga catagtcctg gcgcatgact
     2221 tcgaggacgc tggaacgcga gatccgggtg acgatcgccg ccagcgccga cccgagggtg
     2281 aacgccggca tgagcagatg ctgcagcacg ctccaaaaag cggcgaagtt cgcagtgagc
     2341 aagctgtcga gcagataaag gccggtgacc gttttgacat cgacgccgta ctcgatccgg
     2401 ccggccaccg gtaagagatt taattgccca ccaagaaaat aaataagcat gatgccaagc
     2461 cagaaattcg gcagcgatac accgatcagc gcgatgctca tgcccgcgtg atcgaaaagc
     2521 gaatcatgct tgatcgcgga gtaaacgccc agcgggaccg cgattatgat cgcgacgcaa
     2581 agactggcaa atgccagctc gagagtcgcg ggcagccgct cggcgatcag cttggtcacc
     2641 gattcattga acttgagcga cctgccgaaa tcgcctcgaa cggctcggtt cagaaattcg
     2701 taatactgga cgatgagcgg ttgatccaat ccccactcat gccgggtgcg ctcgatgtcc
     2761 gcgggcgttg cgtcatcccc cagcagcaag gtcaccggat cgcccggcgc gatatgcatc
     2821 aaaaggaaaa tcagaaaaga gattccgagg agaaccggaa tcgctccaat cagcctgcga
     2881 cccagtgtat tcatgcaggt tcgaactcaa gaaagggatg cgcggccatc acccggcaag
     2941 aaaatcggtc ggtccaagaa gcggcgaccg gctactgtcc caattggctg aacgattgat
     3001 ccagaacact caaggcatga tcaatttgat ccttattgac attcaaggca ggtgctaccc
     3061 ggagcacgtt cccgtaggta cctcccttgc cgaccagtaa gccattgcgt ttggtcagct
     3121 ccatcacacg tttcgcgctt tccgcatcag gcagcttctt ttcaccgacc aattccatac
     3181 cttgcatcag acccataccc cgcacatcgc cgatcaccgg gtatttctct tttaacccat
     3241 tcaggccttc acgcaatcgg ttacccataa ggcgagcgtt ttcgaccaaa ttgttttcct
     3301 cgatgacctg gatcgttgcg ctcgcggcgg tgcaggttac cggattaccg ccgaacgtcg
     3361 atatggtatt gcctttcatg ctgtcagcca cttccggagt agcgatcgtc gcgccgatcg
     3421 gtacgccatt ggccatcccc ttggcgaagg tcatgatatc gggatcgaca ccccaatgtt
     3481 cgatgccgaa cattttgccg ccggtgcggc cccagcctgt ttgcacctca tcgctgatga
     3541 acagaccgcc gtatttgcga atgatcccga cgatctcttt aaaatattct ttcggaggaa
     3601 cgataaaacc gccgaccccc tgaatcggtt cggcgagaaa cgcggcgacc cgcccctgcg
     3661 aagtcatggt ttgtatcgct tcttcggcat ccttggcgca tttcagatcg cagctcggat
     3721 aagtcaagcc gaaggggcag cgataacagt aggcattggc gatgtgatga acccccggaa
     3781 cgacattggg cgtgagccgc cagctcgact gtgccgacaa gctcaaggca agataggaac
     3841 ggccgctata ggcgtgtcgc agagtgatca catcctggca cttcgtatga agctgcgcca
     3901 gcaaaacggc ggtctcgttc gcttcggtgc cgctgttggt aaaaaacgat ttctccaagc
     3961 gccccggcgt gatccgggcg agtctct
//
LOCUS       DASULZ010000361         5803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_653875, whole genome shotgun sequence.
ACCESSION   DASULZ010000361 DASULZ010000000
VERSION     DASULZ010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5803
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653875"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="VFY96_07425"
     CDS             complement(<1..1044)
                     /locus_tag="VFY96_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX5606327.1"
                     /translation="MELLNHLLLGLQTAASLSNLFYCFIGVFLGTAIGVLPGLGPTAT
                     IAMLLPVTFVLPPVTALIMLAGIYYGSQYGGSTTSILVNLPGEAASVVTTLDGYQMAR
                     QGRAGAALAASALGSFFAGTVATFLIAGFGPPLASVALQFGPAEFFSLMVLGLVASVV
                     LAQGSLLHAIGMVVLGLLLGLIGTDVNSGTQRFTFGIYELADGIGFVTVAMGMFGLGE
                     IIRNLENEEERSVMITKITGLWPTREDFKRMVAPILRGTVIGSALGILPGSGSILGSF
                     AAYSIEKKVSKNRAQFGHGAIEGVAAPESANNAGAQTSFIPLLTLGIPSNPVSALMVG
                     AMIIHGIQPGPSVI"
     gene            complement(1065..1517)
                     /locus_tag="VFY96_07430"
     CDS             complement(1065..1517)
                     /locus_tag="VFY96_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX5606328.1"
                     /translation="MKRNPKDFWTGLLYIFFGASAVIIARDYSMGTAFRMGPAYFPSI
                     LGAVLAVIGAISVIRSFVAPGTPIGGFTFKGLALVIISVVLFGLLVRDAGLVVALPLL
                     VIISACASAHFRWRTAIIMAAGLTLFCAVVFVKGLGIPLPIIGPWLAG"
     gene            complement(1660..2634)
                     /locus_tag="VFY96_07435"
     CDS             complement(1660..2634)
                     /locus_tag="VFY96_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein BugD"
                     /protein_id="HEX5606329.1"
                     /translation="MSLKKLAAVTTFLLISAIPLYAQNYPNKVITVVVPFAAGGPTDT
                     VARLIGVPMTKTLGQQIIVENVAGAGGTIAANRVAKSPPDGYTILIHHIGMSTAPALY
                     RKLPYNPMTDFEPIGLINEVPMTLVARKDFTAKDLKELIAYVKANKEKVNYANAGLGA
                     ASHLCGMLFMSAIQTEFTTVPYGGTGPAMNDLLGGQVDFMCDQTTNTTSQIKAEKIKV
                     YGVTTKKRVPSLPNVPTMDEAGLKGFEVSVWHALYAPKGTPKPVIDKLTTALQDALKD
                     STVKQRFADLGTEPIALERATPEALRKHLKSEIDRWTPIIKKAGVYAD"
     gene            3130..3381
                     /locus_tag="VFY96_07440"
     CDS             3130..3381
                     /locus_tag="VFY96_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606330.1"
                     /translation="MLFEYLARLTKEKEGETEMKSTESDLEKPSYWQLVFLQSKVNGA
                     AYSGEELPGRNWDWPKDTYLQRRLRTHKFLHPTEYSKLN"
     gene            3666..4613
                     /locus_tag="VFY96_07445"
     CDS             3666..4613
                     /locus_tag="VFY96_07445"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606331.1"
                     /translation="MKLHQVIVAVLVLLGLVLPAQAQEKVVVGHSARAALSIGPLLYG
                     IERGFYRDEGIDLVYVSIRADLGIKALLSGDIDYSYSTGTIIRGAMMGLPVRNLSFDF
                     SRVLHALMSRPEIPNAAALKGKKVGVSSFGATGDLAARVGLRSLGLDPEKDVTIITLG
                     ADTLRHAALLAGTVQATHMPVPLNIQLKKEGYHELVYAGKILQRPLTGLATSLEKIQK
                     NPGQVQRMVRAFLRATRALKNERAGFIAFAQKKYGYSKEVMEEAYNYLIEALSQDGFV
                     DDAALQAAIDEAKSLAKITKPISQSDVVDYSFLRTATRK"
     gene            complement(4616..>5803)
                     /locus_tag="VFY96_07450"
     CDS             complement(4616..>5803)
                     /locus_tag="VFY96_07450"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5606332.1"
                     /translation="GALLNSAALRAARLQPGTADPPGGKFERNSTTGELTGWVKGVPA
                     WKYVYDQIPRLSFDQMRQSLKNCFFELSRLGVTSITDFETPEVNFAHRRLLSDMARTG
                     ELPVRINFYLVPESSVELDYFKSAMDEIKGLMQNDIFRFAGFEGSALFPSDGHDRPAL
                     SDRSLITREREESFRKTASFLLESKYHFQVDVGGDDAADRWLDVLEQINASTPLTQRR
                     IGFAGLETATPETLARIKKLAGGIIVDDRLVLTGESYAEQWGRDKARRAPPLRTIVDS
                     GMPLGAGTSGFQSSAFSPILSLWWLITGKTVAGSPLRAPSANLTRSEALRAHTIGNAW
                     FTFEEGRKGSIEVGKLADLAVLSGDYLTIAEDQIPTLESILTIVGGQVMYAAGPYRGG
                     IRK"
BASE COUNT         1363 a         1619 c         1560 g         1261 t
ORIGIN      
        1 aatcaccgac ggcccgggct gaatgccgtg gatgatcatc gctccgacca ttagcgcgct
       61 caccggattg gacgggattc ccaaagtgag cagcggaata aaagatgttt gcgcgccggc
      121 attgttggcc gactcgggag ccgcaactcc ttcaatcgca ccgtggccga actgggctcg
      181 attcttggaa actttttttt caatcgaata cgcggcgaac gaacccagaa tcgaaccact
      241 tccaggaaga atgcctaagg ccgagccgat cacagtgccg cgcagaatcg gtgcgaccat
      301 ccgtttgaaa tcttcgcgcg tgggccacag tccggtgatt tttgtgatca tgacggagcg
      361 ctcttcttcg ttttctaggt tgcgaatgat ttcacccaga ccgaacatgc ccatcgccac
      421 ggtgacgaag ccgatgccgt ccgcaagctc gtaaatcccg aaggtgaagc gctgggtgcc
      481 ggaattgaca tcggtgccga tgagacccaa gagaagaccg agcacgacca tgccgatggc
      541 atgcaacagc gagccctgcg caagcaccac ggacgccact aacccaagca ccattaagga
      601 gaaaaattcc gccggcccga actgcagcgc caccgaagcg agcggcggcc caaagccggc
      661 gatcaaaaat gtcgccaccg ttcccgcgaa gaacgagcca agcgccgatg ccgcaagcgc
      721 tgctcccgcc cttccttgcc ttgccatctg gtagccgtcg agcgtcgtaa ctaccgaggc
      781 ggcttcgcct ggaagattga cgagaatgga ggtcgtcgag ccgccatact gcgagccgta
      841 ataaataccc gccagcatga tgagcgctgt caccggcggc aatacaaaag tgaccggcag
      901 cagcatcgcg atggtagcgg tgggtccgag gccgggcagc acgccgatcg ccgtgccgag
      961 aaaaacaccg atgaaacagt agaacaaatt gctgagcgat gcggctgtct gcaatcccag
     1021 gagcagatga ttgagtaatt ccatcgttgt ccgtcagtgt ttcatcaacc ggccaaccag
     1081 ggaccgatga tcggcagcgg aatgcccaat cctttaacga atacgaccgc acaaaagagc
     1141 gtcagccccg ccgccatgat aatcgcggtt cgccagcgga agtgcgcgct cgcgcaagcg
     1201 ctgatgatca cgagaagcgg tagagcaacc accaatccag cgtcgcgcac cagcaagccg
     1261 aagaggacca cagatatgat gaccaacgcc aatcctttga aggtaaatcc accgatcggg
     1321 gtgcccggcg cgacgaagga tcgaatcacc gagatcgcgc caatcacggc aagcacggcg
     1381 ccgaggatac tgggaaagta cgccgggccc attctaaagg ccgtgcccat gctgtagtcg
     1441 cgagcgatga tcaccgcgct cgcgccaaag aaaatataga gcaagccggt ccagaaatct
     1501 tttggattgc gcttcataat ggctttcaat cgcgtgacta atgtccggag tagttaccgt
     1561 gctatgcgct ttgtataaca agcggtcgtt gaaaaaaagt aaaaaaaaag gccggggctt
     1621 cgcccggcct tggcacgctg attcgatgaa cgcgatcaat tagtcggcat agacgccggc
     1681 tttctttatg atgggcgtcc atcgatcgat ctcggatttg agatgcttgc gcaatgcttc
     1741 cggggtcgcg cgctctagcg caattggctc ggtgcccagg tcggcgaaac gttgcttgac
     1801 cgtcgaatct ttcagcgcgt cctgtaaagc cgtggtaagc ttgtcgatga ccggcttcgg
     1861 tgtgcctttg ggcgcataga gcgcatgcca aactgaaacc tcgaatcctt tcaagccagc
     1921 ctcgtccata gtcggcacat tgggcagaga cggcacgcgt tttttcgtcg tcacgccgta
     1981 caccttgatc ttttccgctt tgatctgact ggtcgtgttg gtcgtctgat cgcacataaa
     2041 atcaacctga ccgccgagga gatcgttcat ggccgggccg gtaccgccat aaggaacggt
     2101 ggtgaattcg gtctgtatcg cgctcatgaa gagcataccg cagaggtgcg aggccgcgcc
     2161 cagacctgcg ttcgcataat tgactttttc cttattggct ttgacgtagg cgatcagctc
     2221 cttgagatcc tttgcggtaa aatcctttct agccaccagt gtcattggta cttcattgat
     2281 gagaccgatc ggctcgaagt cggtcattgg attgtatggc agcttgcgat agagagccgg
     2341 cgccgtggac atcccgatgt gatggatgag aatcgtgtag ccgtccggcg gagacttggc
     2401 gacacggttg gccgcgatag tgccaccggc gccggcgacg ttttcaacaa tgatctgctg
     2461 acccaaggtc tttgtcatcg gcacgccaat cagccgggcc acggtgtccg tcggtccacc
     2521 cgcagcgaaa ggaacaacca cagtaattac tttgttgggg tagttttgcg catacaaggg
     2581 tattgcgcta atcaggagaa acgtcgtaac agctgcgagt ttttttagtg acatcttagc
     2641 ctccgcattg ggcgtttgcg ctaatcagtt aaatcactaa ttcctgccag atttcctttt
     2701 gtgtgagccg agtatgggaa tgccaagtcc caaaccaaag ttttacgatt agttagttag
     2761 ttctttctaa tgtctcccaa aaccgctaaa ccaatatggt ttcctcgcaa ccaacacgct
     2821 cgaacaagcc gcaacgaggt ttctgccttc tgttttcacc ttacatgggc tgactaaggc
     2881 attcaatgat gcactagtcc ctttctgggc gctcaggaac catccatcct ggcagttttg
     2941 accgaaggat ggggataggc agtgagcccg gtccgtcatg cccctgagaa tgcgtgaatt
     3001 ctttctgaca atcgatgaca cagagtgctg cgtaaggtgt tctccgcgcg cggcatgcct
     3061 tctgcttttc tgcccgtgag caagcaacaa ttcagagtga gttaaaactg aggcttagga
     3121 gaagacggta tgctgttcga atatctcgct cgtctgacaa aagagaagga aggagagacg
     3181 gaaatgaaat cgacagaatc agatctggag aagccgagct actggcaatt ggttttttta
     3241 caaagcaagg taaacggtgc cgcttattct ggcgaggaac ttcctgggcg taattgggat
     3301 tggcccaaag atacttatct gcaacgccgg ctgaggacgc ataaattttt gcacccaacg
     3361 gaatactcta aactgaatta gtgtgccgct tcccgcgctg cctccttcag cacagctccc
     3421 acgggatagc ggctgccact gagcccccgt cacgttcagt ggcaagaggc cagactctca
     3481 agagtctggc ctctcttttt tattgaaata gctgcacctc agctaagcac ttcttcggca
     3541 cgattctaaa ccaggttctt tccacgcatt taggagcgat tgttctcgat agagtttacg
     3601 agccgccgac aaggtgcatc gtggctttgc acggcataaa cgaaattgat aagcacctgg
     3661 ctgatatgaa attacatcaa gtcatcgtcg ctgtgctcgt attgcttggt ttggttttgc
     3721 cggcgcaagc ccaggaaaaa gtggttgttg gccactcggc ccgcgctgcg ttatccatag
     3781 gaccgctttt gtacggcatc gaacgcggct tctatcgtga tgaaggaatc gatctcgtat
     3841 atgtgtccat tcgcgccgat ttgggtatca aggctttgct ctccggcgat atcgattaca
     3901 gctacagcac gggtacgatc atccgcggcg caatgatggg tttgccggtg cgcaatctct
     3961 ccttcgactt ctcgcgcgtg ctgcacgcct tgatgtcccg accggaaatt cccaacgcgg
     4021 ctgcgctgaa ggggaaaaaa gtcggcgtaa gctccttcgg cgcaaccggc gatcttgcgg
     4081 cccgggtggg attgcgctct ctcggcctcg atccagaaaa agacgtgacg attatcacgt
     4141 tgggcgctga cactctgcgt catgcagccc tgctcgcagg aacggttcaa gcgacgcaca
     4201 tgccagtgcc actgaacatt cagcttaaga aagaaggtta tcacgagctc gtctatgccg
     4261 gcaaaattct tcagcgcccg ctcaccggtc tggcgacaag ccttgaaaaa atacagaaaa
     4321 atcccggtca agtgcaacgc atggtgcgcg ccttcttgcg ggccacgcga gctttgaaaa
     4381 atgagcgtgc cggttttatt gcgtttgcgc aaaagaaata cggttattcg aaagaagtca
     4441 tggaagaagc atacaattat ctgattgagg ctctttccca ggatggcttc gttgatgacg
     4501 ccgcgctcca ggcagccatt gatgaggcaa aaagcctcgc gaagatcact aagccgatca
     4561 gccaatcgga cgttgtcgac tacagttttc tgcggacggc aacaagaaaa taaacttact
     4621 tgcggatgcc ccctctgtac ggaccagccg cgtacataac ttgcccacca acaatcgtca
     4681 agatggattc gagcgtgggg atttgatcct ccgcgatcgt cagataatcg ccgctcaaca
     4741 cggccaaatc ggcaagctta ccgacctcga ttgaaccttt gcgcccttcc tcgaaggtga
     4801 accaagcgtt gccgatggtg tgcgcgcgca gcgcctccga acgcgtgagg ttcgccgatg
     4861 gagcgcgcaa aggcgaaccg gccaccgttt tgccggtaat gagccaccag agcgacagga
     4921 tcggcgagaa tgcgctggat tgaaaaccgc tcgtgccagc gccgagcggc atacccgagt
     4981 ctacaatcgt ccgcaacggc ggcgcacgcc gcgccttgtc ccggccccac tgctcagcat
     5041 agctctcgcc agtcaggacc aaacgatcgt ccacaatgat tccacctgcc aattttttga
     5101 tcctcgcaag agtctccggc gtcgccgttt ccagacccgc gaaaccgatc cgacgctgcg
     5161 tcaacggcgt cgatgcgttt atctgttcga gaacatcgag ccaacgatcc gccgcgtcat
     5221 cgccgccgac gtccacctgg aagtggtatt tcgattctag aagaaagctc gccgttttcc
     5281 ggaagctctc ttcgcgttcg cgcgtgatta gagaacgatc cgataaagcc ggcctgtcat
     5341 ggccgtcaga cggaaagagc gccgatccct cgaaaccggc aaaccgaaag atgtcgtttt
     5401 gcatcagacc tttgatttcg tccatagcgc tcttgaaata atccagctca actgaacttt
     5461 caggcacaag gtaaaagttg atccgcaccg gcaactcgcc agtgcgcgcc atgtcactta
     5521 gaagacggcg atgcgcaaag ttcacttccg gtgtttcgaa atccgtgatc gatgtaactc
     5581 ccaagcgact taactcaaaa aaacaatttt ttaggctctg gcgcatttga tcgaaagaaa
     5641 gccggggaat ttgatcgtaa acatacttcc aggctggcac gcccttcacc catcccgtca
     5701 gctctccggt tgtcgaattg cgttcgaatt ttccgcccgg cggatccgca gttcctggct
     5761 ggaggcgggc tgcgcgcaac gctgcgctat tgagcagagc gcc
//
LOCUS       DASULZ010000362         2768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_45702, whole genome shotgun sequence.
ACCESSION   DASULZ010000362 DASULZ010000000
VERSION     DASULZ010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45702"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VFY96_07455"
     CDS             complement(<1..301)
                     /locus_tag="VFY96_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606333.1"
                     /translation="MRLGAIHRLCPILAALLMLLNGCGNAGHRVLEEVVEKVYTIEPD
                     ANVSIKNGDGAVLVYGSDNNEIRVRCVKRAYSHERLNQIAIDISTKPGGVSIITRF"
     gene            complement(388..1923)
                     /locus_tag="VFY96_07460"
     CDS             complement(388..1923)
                     /locus_tag="VFY96_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX5606334.1"
                     /translation="MLAKIYSAAVYGVDAYEVEIEVNGAGGEPVIVIVGLPDAAVKES
                     RDRVTTAISNSGYYWPRGRTTINLAPADIKKEGPSFDLPIALGMIAVTEGLDSSPFEN
                     FSFVGELALDGSVRPVKGVLPVALEARRRGKRALFVPEANALEAAMVDKIDIYGVQNL
                     RQTFAFLRGEIALLPTRADLSKFFAAHQSYDVDFSDVKGQGHVKRAIEVAVAGGHNVL
                     MIGPPGSGKSMLAKRIPTIIPPLTLDEAIETTKIHSIAGLLDTEQSFVSTRPFRPPHH
                     TISDIGLLGGGAFPNPGEVSLAHNGVLFLDELPEFKRSALEVMRQPLEDGKVTVSRAA
                     GSVTFPSEFMLVAAMNPCPCGYFGDLKRECRCGPVQVQRYRQRISGPLLDRIDLHIEV
                     PAVEYRDVASERAEETSAAIRKRIARGRKRQQERFRNETKINCNARMATRQLKQHCKL
                     SADSQELIRVAMSELNLSARAYDRILKVSRTIADLDGKPEIAPEHVSEAIQYRTFDRT
                     LWV"
     gene            2223..>2768
                     /locus_tag="VFY96_07465"
     CDS             2223..>2768
                     /locus_tag="VFY96_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5606335.1"
                     /translation="MKKSYMAAEDSDTGIKIYLREIGQIPLLTPEQEIELAAKIKKGD
                     REARALMIRSNLRLVVKIAHDYANLGLPLLDLISEGNIGLMKAVERFDPAKGGKLSTY
                     AAWWIKQSIKRALANQSKTIRLPVHLVDKISKMRRVSLQMSEELGREPTDEELGDEIG
                     IASGKVSQLTTVSIRPASLDAP"
BASE COUNT          631 a          749 c          780 g          608 t
ORIGIN      
        1 gaaacctcgt gatgatcgaa acgccgccag gttttgtgga gatgtcgatg gcgatctggt
       61 tcagacgttc gtggctgtac gctctcttaa cgcatcgcac ccgtatctcg ttattatctg
      121 agccgtaaac caggacggcg ccatcaccat tcttaatgct tacgttcgcg tcaggttcga
      181 ttgtataaac cttttcgaca acctcttcga ggacacggtg acccgcgttt ccgcagccgt
      241 tgagcaacat caacaatgca gcaaggattg gacagagtcg gtgaatagcc cccaatcgca
      301 cggtcgaatc ctatcagtgg ctattcgctt tcgaaagcga cacgagaaca cgcgcttgct
      361 ccacgctgcg agaaatgatt tgcgcgctta gacccaaagc gtccggtcga aggtgcgata
      421 ttggatggct tcgctgacgt gctcgggcgc gatctccggt tttccgtcga gatcagcaat
      481 agtacgcgag actttcagga tacgatcgta ggcgcgggcg ctgagattta attcactcat
      541 ggccacacga atcaattcct gggagtccgc gctcaactta cagtgctgtt tcaactggcg
      601 tgtggccata cgcgcgttgc aatttatttt cgtctcgttg cgaaaccgtt cctgttgtcg
      661 ttttctgcct cgcgcgatgc gcttgcgaat ggcggcggac gtttcctccg cgcgttcgct
      721 ggcgacgtcg cggtattcca cagcgggcac ttcgatgtgt agatcgatgc gatcgagcaa
      781 cgggccagag atgcgttggc ggtaacgctg cacctggacc gggccgcagc ggcattcgcg
      841 ttttagatcg ccaaagtaac cgcatgggca gggattcatt gcggcgacga gcatgaactc
      901 ggagggaaaa gtgacgctgc ccgccgcgcg cgatacagtg actttcccgt cctcgagcgg
      961 ctgacgcatc acttctaatg ccgatcgctt aaactccggc aattcatcca gaaacagcac
     1021 gccattatgc gcgaggctga cttcgcccgg attgggaaaa gcgccgccgc caagcagacc
     1081 gatgtcgctg atcgtgtgat gcggcggccg aaaaggtcgc gtcgacacaa aggattgttc
     1141 ggtgtcgagc aacccggcga tgctatgaat cttggtcgtc tcgatcgctt catcgagcgt
     1201 gaggggcgga atgatcgttg ggatgcgttt ggcgagcatt gatttgcccg aacccggcgg
     1261 gccgatcatt aggacattgt gaccgccggc gacagccact tcgatcgccc gttttacgtg
     1321 accctggccc ttcacgtcgc tgaaatcgac gtcgtaactt tggtgcgccg cgaagaattt
     1381 gcttagatca gcgcgtgtcg gcagaagtgc aatttcgccg cgcaaaaatg cgaaagtctg
     1441 ccgcaaattc tgcacaccat atatgtcgat cttgtcgacc attgcggctt cgagcgcatt
     1501 tgcctcgggc acgaacaacg cgcgcttgcc gcggcgccgc gcctcaagcg cgaccggcaa
     1561 cacgcctttt acgggccgga cagaaccgtc gagagccaat tcgccgacaa aactgaaatt
     1621 ttcgaacggc gaactgtcga gtccttcggt cacggcgatc attccaagcg caatgggcaa
     1681 gtcgaagctc ggcccttcct tcttaatatc ggcgggggcg agatttatgg tggtccgtcc
     1741 gcgcggccag tagtagccgc tattcgaaat cgcagtcgta acgcggtcgc ggctctcttt
     1801 taccgccgcg tctggaagcc cgacgatgac tattaccggt tcgccgccag cgccgttaac
     1861 ctcgatttcc acttcgtaag cgtccacgcc gtataccgcc gcggagtaga ttttcgccag
     1921 catgcaagcg gcagagttgc agaaaatcgg tgcgccgaaa agcgccaaat gttcctgcaa
     1981 agggagatgc aacggatgtg tcaaagtgtt gtcaaatatc aacaaatgcg tcggacaacc
     2041 tcgtatttac cgcccagaaa aacccctaga aaaaaatttg aacgacgggc atccaatcgg
     2101 tgtcacaaat tcgaatattt aaccttgtta aatacttcct cggatggtac aacgcgtgat
     2161 cgataaggcg tcgacgaata caatgtacgg ggagttgtcg aacctgtccc tccggatgaa
     2221 caatgaaaaa gagttatatg gctgctgaag atagcgatac aggaataaag atttacctgc
     2281 gcgaaatcgg gcaaatcccg ctgttgactc cggagcagga gattgagctc gcggcgaaga
     2341 tcaaaaaggg ggatcgtgag gcgcgcgcgc taatgatccg ctccaacttg cgtttggtcg
     2401 tcaaaatcgc gcatgattat gcaaaccttg gtctgccgct gctggatttg atctcggaag
     2461 gcaacatcgg cctcatgaag gcggtggagc gttttgatcc cgcaaagggt ggaaagctaa
     2521 gtacttacgc cgcgtggtgg attaaacagt ccattaagcg agcgctggct aaccaaagca
     2581 agaccatccg tttgcctgtg cacctagtgg ataaaatctc caagatgcgt cgcgtgtcgc
     2641 tgcagatgag cgaagaactc ggtcgcgaac cgaccgatga agagctaggc gatgagatcg
     2701 gcattgcgag cgggaaagtt tctcagctga cgacggtctc aattcggccg gcttcactcg
     2761 acgcgccg
//
LOCUS       DASULZ010000363        12895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_146263, whole genome shotgun sequence.
ACCESSION   DASULZ010000363 DASULZ010000000
VERSION     DASULZ010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 12895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12895
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146263"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1410)
                     /locus_tag="VFY96_07470"
     CDS             complement(<1..1410)
                     /locus_tag="VFY96_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5606336.1"
                     /translation="MIDGEWDVILIGAGQNSFALGTYLGKAGLKTVICESRLENGGRL
                     ASEEITMPGYWHNTLAYFQDNREVSPVWKDLDWANRYHAEFVSPAVLSSLLLQDGRSI
                     AQHQSLERTISSIGNFSAQDAGAWRNIHGRVHRFFVGYVIPYYHTRPENTETLLQRLE
                     AEPEGKEFRRLWQSTPQQVVNELFESEAVKTLILAQMAIPRGVALDYAGAGIEVLKMI
                     AGDEKPELARGGSHSIAQVLQRAYVHNGGQIRAVHHVEKILVDNGGRAFGVRLRDGRE
                     WKARLAVVSNSDPYSTFVEMIGEEYLPRTFIERVKDIKADEFSYFQVHLALKAPVRYA
                     WHEAGDASVSKAMSVSMGPERPADLAELWKEIRAGEFPAHPCLHAICPTAFDPLQAPK
                     GRHAASAFLPVPYRLKGKNPQDWVKLKNGFMEDVLKIWRRYATNLTDENIEMKVALDP
                     FYIAGRWPNMRCGSVWVARK"
     gene            complement(1477..2493)
                     /locus_tag="VFY96_07475"
     CDS             complement(1477..2493)
                     /locus_tag="VFY96_07475"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5606337.1"
                     /translation="MNKQHCKLIFAVILALLSTGRVLAQDAKLLDAAKQEGGKVVVYG
                     SLENDTMDLISAAFKKKTGLDVDYWRDAANKVTDRVAAEFRAGKPQADVVLTTTSTLR
                     LIQKDGLLAKYDSPSAKAFPKNVVDANLGPAYRSTVIGVVYHTGIIKPAEAPKSLEDI
                     TRPQYKGKVVFPDPSQHTATAQWLASLHKIMGKERANKFIQDLAASKPLLVPSLTPAG
                     ERITTGETPIGVAFLKNVVFYGRNGVPLDYSRLGKFMGDGQSITLAAKAPHVSAGKAF
                     VDYFLGEESLKIMANIGEFVTRKGIYPPLPDADKIQLVDMIDMNKEAFAQKMAEYRKI
                     FLQQ"
     gene            2600..3718
                     /locus_tag="VFY96_07480"
     CDS             2600..3718
                     /locus_tag="VFY96_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5606338.1"
                     /translation="MNAREKLKSIREELKQTFLERSDLIDGALAALLSSHHLLIIGPP
                     GTAKSMLADELCRRIHGANYFQWLLTRFTTPEEIFGAVSLKALEQDDYRRVTSRKLPE
                     AHIAFLDEIFKANSSILNAILTLINERLFHNGKEIGRVPLLTLFGASNELPEEEELTA
                     LYDRFLVRFVVGYISEDFRFLRMLESQRAPQRTILTLAELQDMQQQVRDVALPAHVYR
                     SIADIRRELNKKNIQASDRRYRQALPFLQAYAYLEGENEVYEKHLFFLEHVLWRDPAE
                     HEPVRNTIRELLLGYEEEITELLYESREIRDSALQSARTSDERARALIEFHTKLRNIL
                     GKVDQIMDKAKRLGRPLERVNNVRHEIEQLQKEMLEQF"
     gene            3718..5292
                     /locus_tag="VFY96_07485"
     CDS             3718..5292
                     /locus_tag="VFY96_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606339.1"
                     /translation="MASRRAPRAPVKRVFEPLPKNSFWILNDAYDRRVWQQLRAESPS
                     LRELEEKGAAFLPHFGSLLQDIFCLLFKYNINYFEPRDVLASALLNEKFLRSMHQGAQ
                     YEFLREQTLLNEAHAGLSTLILGERLLALVREEKLLTRRDMRDLWDIQKQEEIVSQKM
                     DEYENADTIPADQMSEEGKKALEQAKQRMGGEAQGAEALLRQKTQRLKEDLEQIEAQA
                     TNRLQAEAIKIAQELEDATEETAAWGDTIGTGQRTPPGQKLELGRRLAGNEKLKKLAR
                     MVGRMKFNALALRKKVFERSNNELLEVEQGDALHRLLPNELLTLHHPVLRKDFYRRFL
                     DQELIQYSLRGVEEKGKGPMIVCLDGSSSMSGDKEIWSKAVTLTLLEIARKQRRLFRS
                     ICFSSADTPLQTLDMNPRDRYEIETKAVMDLAEYFPGGGTDFQKPLDAALDCLRQSRF
                     KKGDIVFITDGECQVDPQWAENFRAQKERLGFSLFSILIDTGPAALGTLKEFSDRIST
                     IKQLTGEEAKDIFVKF"
     gene            5327..5494
                     /locus_tag="VFY96_07490"
     CDS             5327..5494
                     /locus_tag="VFY96_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606340.1"
                     /translation="MIDIRLNEKAGFVAGLLSLNQLPNGNLLMSLKPHALQAAPNFTY
                     CSVFSAAFCGK"
     gene            5830..6267
                     /locus_tag="VFY96_07495"
     CDS             5830..6267
                     /locus_tag="VFY96_07495"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5606341.1"
                     /translation="MESITKITVVDTEKQAQWGKPTGRNYVRPTQTDRAHIALNCYEP
                     GVNDELHCHPGSDHTFFVVQGECTMKGLREGEEFHLKQHQAVHVPAGFFYQLNNTGTE
                     RLILYQVSTEPKPKPKIGKVIYGIHTGLKEHLLKPVARPTQRS"
     gene            6264..6521
                     /locus_tag="VFY96_07500"
     CDS             6264..6521
                     /locus_tag="VFY96_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606342.1"
                     /translation="MTFTDILQWCRQHHAVVRGVYRGGAISISHEDKELPAEIPAFDD
                     IFHWGIDMPELKHYVSGSDFERMVTGKLTIDGFKSSLRGRE"
     gene            complement(6605..7363)
                     /locus_tag="VFY96_07505"
     CDS             complement(6605..7363)
                     /locus_tag="VFY96_07505"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5606343.1"
                     /translation="MRIIERAFSAKEHGVAGFLSYPERQEAGPALFLIHPKGGLGEYI
                     KTETRKFARLGYTTFAINVFEQLGYPEPTHIVSGSQIQAKTPDPEFTRVLTEGWRFLL
                     KQPNVNKDRVAVCGYCMGGRIAIHFVAATPEVRAFVGYYPTVRAEPDSPLRPVHPSAS
                     ARKIRCPSVILYGADDVTTVLPVQEQMWQAFKENRQSLEWHFFPFGGHGYVDPGAPGY
                     NAHTADLSWPLVTDFLERHLKWNSPEVVNPNLPA"
     gene            7671..8510
                     /locus_tag="VFY96_07510"
     CDS             7671..8510
                     /locus_tag="VFY96_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606344.1"
                     /translation="MALPATPSFSLAEANSIVEIEVQADQLLGYIDKLTTLADRACTT
                     AIRQSQTAHLIDASRHGEINDLRKKLDQQNEKLHEQEIAIVRLQHESKAQIAAVEAQL
                     RQNHLQPRHEPEQHLLRYENAQPVKRLNDSPEIAATKVEPDHTRFHTANDPTIAEFKL
                     QIANRDETILAKTNALKAIEEEYRARIAHLEQRLQETTALLEKHAEELNEKDRIIEAT
                     ACKEAEMGNLIKQLSAECTALSEELQKSDPATARGEAKPNPTVNESTLWRRVLGRLQE
                     EPQ"
     gene            complement(8596..9687)
                     /locus_tag="VFY96_07515"
     CDS             complement(8596..9687)
                     /locus_tag="VFY96_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEX5606345.1"
                     /translation="MRHQRNKVVKVAPIRAVKSRGLRYVMDSIKGITRRRHGKNFLFF
                     SPRGVPIRNAGELRRIRALAIPPAWKDVWISPFSNSHLQATGRDARGRKQYRYHHAWR
                     EWRDQTKYDHLMEFGRVLPKLRRKVAKDLSLPGLPRAKVMATIVRLLETTCIRIGNDE
                     YVRQNQSFGLTTLRNKHVKVRGSKIHFRFRGKSGIVRDVDLNDHRLAQIVRHCQDLPG
                     QELFQYLEEDGTPRIVNSSDVNQYLREATGVDLTAKDFRTWAGTVLAARALGELPPFA
                     SHSQAKRNVVQAVEAVARKLGNTRAVCQKCYIHPATVKAYFDGSLPENLSRPVPAARQ
                     AQHGLSDDEAAVMMILEESSKAATEKKVA"
     gene            complement(9933..12056)
                     /locus_tag="VFY96_07520"
     CDS             complement(9933..12056)
                     /locus_tag="VFY96_07520"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX5606346.1"
                     /translation="MIPFVIAISCLSLAFAVFLARHVIQRDAGTAQMQQISNAIKEGA
                     EAFLSRQNRTIISFSAVLAALIFVLYAYARTPSPADPVPPVQLAFWTTLSFVLGAACS
                     VIAGYVGMWVSIRSNIRTASAVRSSLNDGLQIALRGGAVSGLFVVAMSLLGVGGLYAL
                     LEALGHDPQKIPFTIVGYGFGASFVALFAQLGGGIYTKAADVGADLVGKVEAGIPEDD
                     PRNPAVIADLVGDNVGDCAGRGADLFESTAAENIGAMILGVGLFPYFGLKGIMFPLVA
                     RAFGLLASIVGVMVVKCKETEDPMAALNRGYYVTSALAIAGFLVSAKWLLAVDPGTHP
                     EATSAWLYFFWCGVIGILMAQAFVYITQYYTEAKYRPVKSIAEASQTGPATNIIAGVA
                     VGLECTAIPVIVISIAIIASYYLGGMSGVPHAGLFGTAVATMGMLGTAAYILAMDTFG
                     PITDNAGGIIEMSGQPEEVRQKTDRLDAVGNTTKALTKGYAIGSAALAAFLLFSAYLD
                     EVAHYGAKLEAVNIARPEVFVGGLLGATLVFLFSALAIRAVGKAAYYVINEVRRQFKE
                     NPGILQGTAKPDYGRCVDIVTVGALKEMVLPGAIAVGTPVAVGVIFRLFGVGAEAVAA
                     LLMVGTIAGILMATFLNNSGGAWDNAKKFIETGAFGGKGSDPHKAAVVGDTVGDPFKD
                     TAGPSLHVLIKLLSTITLVLAPLFV"
     gene            complement(12086..12796)
                     /gene="tatC"
                     /locus_tag="VFY96_07525"
     CDS             complement(12086..12796)
                     /gene="tatC"
                     /locus_tag="VFY96_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004509446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX5606347.1"
                     /translation="MTLTEHLEDLRWCLLKSVLAIVIAAGGSYFFSEPIFNFIVAPLR
                     QNLKPGQNLIGTSVTEAFFVEIKVALAAGILFSCPVIFYQIWRFVAPGLSQHERKWVV
                     PFGFCATLFFVAGASFCYRFVLPVAFSYFIQQYETMGVSPAIRIGDYFTFFFRMVGAF
                     GLTFELPVFTFFLVRIGVWDYHFMLRSFRYAIVLIFIIAAVLTPTPDVINQCLLAGPM
                     LLLYLLSIGVAYVWRKPG"
BASE COUNT         3157 a         3683 c         3335 g         2720 t
ORIGIN      
        1 ttttcgcgcc acccacaccg atccgcaccg catgttcggc caacggccgg cgatataaaa
       61 tggatctaac gccactttca tctcgatatt ttcatccgta agattggtcg cgtaacgccg
      121 ccagattttt aagacgtcct ccataaaacc attcttgagc ttcacccaat cttgggggtt
      181 tttgcctttc agccgatatg gcactggcaa gaatgccgac gccgcatgtc tccccttcgg
      241 ggcctgcagc ggatcgaatg cggtagggca aattgcgtgc aggcagggat gcgccggaaa
      301 ctcacccgcg cggatttcct tccacagctc ggctagatcc gccggccgtt ccggccccat
      361 gctcacgctc atcgctttgc ttacagaagc atcccctgcc tcatgccagg cgtaacgcac
      421 tggcgccttt agcgccaggt gaacctggaa ataagaaaat tcatcggctt taatatcttt
      481 taccctctcg atgaaggttc gcggcaggta ttcctcgccg atcatctcga caaaagttga
      541 ataaggatcg ctgtttgaaa cgacggctaa gcgcgctttc cactcacgtc catcgcgtaa
      601 cctcacgccg aacgcacgtc cgccgttatc gacaaggatc ttttccacat ggtggaccgc
      661 gcggatctgt cccccgttgt gaacgtaagc acgctgtaat acctgcgcga tcgaatggga
      721 gccgccgcgc gctagctcgg gcttttcgtc gccggcgatc atttttaaaa cttcaatgcc
      781 ggcccccgca tagtccagtg ccacgccgcg cggtatcgcc atctgcgcca ggatcaaagt
      841 tttaaccgct tcgctttcga atagttcatt caccacctgt tgcggcgtcg attgccagag
      901 acgccgaaac tctttaccct ccggttcggc ttcaagacgt tgcaaaagag tctcggtgtt
      961 ctcgggccgg gtgtgatagt aaggaatgac atagccaaca aagaacctgt gaactcgtcc
     1021 atgaatattg cgccacgcgc cagcatcttg ggccgaaaaa ttaccgatac ttgatattgt
     1081 tcgttcgagg gattggtgct gcgcaatcga tcgcccgtct tgcaaaagca gcgagctcaa
     1141 taccgctgga gaaacaaatt cagcatggta ccgattcgcc cagtcgagat ccttccagac
     1201 gggtgaaact tctcggttat cctgaaaata ggcaagagtg ttatgccagt agcctggcat
     1261 cgtgatctct tcgctcgcca aacggccgcc gttctccagc cggctctcac agattaccgt
     1321 tttcaggccc gctttgccga ggtaggtacc gagggcaaaa ctattttgcc ccgcaccgat
     1381 caaaatcaca tcccattcgc cgtcaatcac gcaaaggttt cctcgtgagg cttcaaactt
     1441 tttgaacggc ttgaacgctt tgaacggatc ggaccttcat tgttgcagaa agatttttct
     1501 atactccgcc atcttttgcg cgaaagcttc cttgttcata tcgatcatat cgacaagttg
     1561 aatcttatcg gcatccggca gcggcggata aatgcctttg cgtgtaacaa actcgccgat
     1621 attggccatg atcttaaggc tttcttcgcc aagaaaataa tcgacaaacg ccttacccgc
     1681 gctgacatga ggtgccttcg cggccaatgt aatcgattgt ccgtcgccca tgaacttgcc
     1741 caatcgactg taatccaaag gcacaccgtt tctgccgtag aacaccacat tcttgagaaa
     1801 cgcgacgccg atcggagttt cgcctgtggt aatgcgctcc cctgcgggtg tcagcgacgg
     1861 aaccagaaga ggctttgagg ccgccaaatc ttggatgaac ttattcgccc tctccttgcc
     1921 catgatctta tgcaggctcg ccagccactg cgccgtggcc gtatgctggc tcggatcggg
     1981 aaaaacgact ttgcctttat actgcggcct ggtgatgtct tcgagagact ttggggcttc
     2041 cgccggctta atgatgccgg tgtggtatac gacgccgatg accgtgctac gataagccgg
     2101 gccgagattc gcatcgacga cgtttttcgg gaacgccttg gccgagggtg agtcatattt
     2161 cgccaacaga ccgtcctttt gaatcaaccg caaggtcgag gttgtcgtca acaccacgtc
     2221 ggcctgcggt ttaccggcgc ggaactccgc cgcgacccga tccgtcactt tattggctgc
     2281 atcgcgccaa taatccacat ccaatccggt ttttttctta aaggcagccg agatcaagtc
     2341 catcgtatca ttctcgagag aaccataaac gacgaccttg ccgccttcct gtttcgccgc
     2401 atccagcagt ttggcatcct gcgcgagtac ccttccggtg ctaagcagcg ccaagattac
     2461 ggcaaaaatc agtttgcaat gttgtttgtt cattttatgt ttgccttccc gaccgatgat
     2521 ttcctcaata tcgaaaagca gcgcgcttgt ctaatgtttg tctcacgtcc gggcgtttgc
     2581 taacttactc gaaacgatta tgaatgcgcg agaaaaactg aaaagtattc gcgaggaact
     2641 gaagcagacg tttctggagc gcagcgattt gatcgacggc gccctcgcgg ccctgctctc
     2701 atcgcaccat ctgctgatca ttgggcctcc cggaaccgct aaatcgatgc tcgccgatga
     2761 gctctgccgc cgcatccacg gcgcaaatta tttccaatgg ctgctcacgc gctttaccac
     2821 acccgaagaa atcttcggcg ccgtgagcct taaggcgctg gagcaagacg actaccgacg
     2881 cgttaccagt cgcaagctgc ccgaggcaca catcgccttt ttggatgaga tcttcaaggc
     2941 caattcatcg atcctcaacg ccatccttac cctcattaat gagcgcctgt ttcacaacgg
     3001 caaggaaatc ggccgcgtac cgttactaac cctctttggc gcaagtaacg agctgccgga
     3061 agaggaagag ctgaccgcac tttacgatcg ctttctggtg cgcttcgtcg tcggctacat
     3121 cagcgaagac tttcgcttcc tccgcatgtt ggaatcgcaa agggctccgc agcggacgat
     3181 cttgacgctc gcagagcttc aagatatgca acagcaggtg cgcgatgttg cgcttcccgc
     3241 ccacgtttac cgaagcatcg ccgacattcg ccgtgagctg aataaaaaaa acatccaggc
     3301 ttccgaccgc cgctatcgcc aggcactgcc gtttttgcaa gcctacgcct atcttgaagg
     3361 cgaaaatgaa gtctatgaga aacatctttt cttcctcgag cacgttcttt ggcgcgaccc
     3421 ggctgaacac gagccagtgc gcaatacgat ccgcgagctc ttgttaggct acgaggaaga
     3481 gatcaccgaa ttgctgtacg agtcgcgcga gattcgcgac tcggcgctgc aatcggcccg
     3541 aaccagcgac gagcgtgctc gcgctttgat cgagttccat acgaaactca gaaatatcct
     3601 gggcaaggtc gatcagatca tggacaaagc aaaacgcctc ggtcgtccgc tggaacgagt
     3661 caacaatgtg cgccatgaaa tcgagcagct gcaaaaagaa atgctggagc agttctgatg
     3721 gcgtctaggc gtgctccgcg agcgccggtc aaacgcgtct tcgagccgct gccgaaaaat
     3781 agcttttgga tcctcaacga cgcctatgat cgacgggtct ggcagcagct gcgcgccgaa
     3841 tcaccgtcgc tgcgcgagtt ggaagagaaa ggcgccgcat ttctacccca tttcggctcc
     3901 ttgctgcagg atattttctg tcttttgttc aagtacaaca tcaactattt cgagccccgc
     3961 gacgtgctcg caagcgcgct gctgaatgag aagtttcttc gttccatgca ccagggggcg
     4021 cagtacgaat ttctgcgtga gcaaaccctt ctcaacgagg cgcatgccgg gctgtctact
     4081 ttgattctcg gcgaacggtt gttggccctc gtccgcgaag aaaagctcct gacccgccgc
     4141 gatatgcgcg acttatggga cattcagaaa caggaagaaa tcgtcagtca gaaaatggac
     4201 gaatacgaaa acgccgatac gattcccgct gatcaaatgt cggaggaagg aaaaaaagcg
     4261 ctcgaacagg cgaagcagcg catgggcggc gaggcccagg gcgccgaggc cctgctgcgc
     4321 cagaaaacac aacgcttgaa ggaggatctg gagcagatcg aggcacaggc gacaaatcgt
     4381 cttcaggccg aagcgatcaa gatcgctcag gagctggaag acgcgacgga agaaactgcg
     4441 gcctggggtg acaccatcgg aaccggacag agaacgccgc cggggcaaaa gctcgagctc
     4501 ggccggcgac tcgccggaaa cgagaaactc aaaaagctcg cgcgcatggt cggacggatg
     4561 aaattcaatg cgctggcttt gcgcaagaaa gtctttgagc gctcaaataa cgaattgctt
     4621 gaagtcgagc aaggcgatgc gcttcatcgg ctgctgccga acgagctgct cactttgcac
     4681 catcccgtgc tgcgcaaaga cttttaccgg cgcttcctcg accaggaact gattcaatat
     4741 tcgctgcgcg gcgtcgaaga aaaaggcaaa gggcccatga tcgtctgtct cgatggcagc
     4801 tcctccatgt ccggtgacaa ggaaatctgg tccaaggcag tcacgctgac gcttttggaa
     4861 atcgctcgca agcaacgtcg cttgtttcgc tcgatctgct tctcgtcagc tgacacgccg
     4921 ctgcaaacac tcgacatgaa tccgcgcgat cgttatgaaa tcgaaaccaa agcggtcatg
     4981 gatctggccg aatactttcc cggcggcggc accgattttc aaaagccgct cgacgccgcc
     5041 ctcgattgtc tccgccaatc acgctttaaa aaaggagata tcgtcttcat cactgacggc
     5101 gaatgccagg tcgatccgca atgggcggag aatttccgcg cacaaaaaga acggctcggg
     5161 ttctcactgt tttcgatttt aatcgacacc ggcccggctg cgctcggcac actgaaggaa
     5221 tttagcgatc ggatctcgac gatcaagcag ctcaccggcg aggaggcgaa ggatattttt
     5281 gtcaaattct gagtcgctcc gctgagtgcg ccaggagtga atccggatga tcgatatccg
     5341 attaaatgaa aaggccggct ttgttgcggg ccttttgtcg ttaaaccaat tgccgaacgg
     5401 caatcttctg atgtcactca agccgcacgc cttgcaggcg gcgcccaatt ttacttattg
     5461 ctccgttttt tccgcggcct tttgcggcaa gtgatgcagt caattttggc agccatttca
     5521 tcctccacgt tagcagagaa agggatacca cgcagtcttt agacgtcaac cggagggtga
     5581 tcttcagaga gatgaattcg gctatttttg ccagcgattt ccatcgacta aaaatattct
     5641 tggcagccag atctcaagtt aggtacgcta cctaaagaat gacttatgaa tatctgttta
     5701 agtatctatt aatatagacg gatgaaactt ataaggcgcc gcggcacaag cgctttcgag
     5761 ctcttgaagg gccaagacct cttcctgtaa attcgacagc gacgacctag tcagtaaaag
     5821 gagcgattaa tggaaagcat cacgaaaatt accgtggtcg ataccgagaa acaggctcaa
     5881 tggggaaagc ctaccggccg caattacgtt cggccgacac aaaccgaccg cgctcatatc
     5941 gcgctcaact gttacgagcc aggcgttaac gacgaattac actgccatcc cggctcggat
     6001 cacactttct ttgtggttca gggtgaatgc accatgaagg gacttaggga aggcgaggaa
     6061 tttcacctca aacaacacca ggcggtccac gtccccgcgg gattttttta ccagctgaac
     6121 aataccggaa cagaacgtct gatcctttac caggtaagca ccgagccgaa gccgaaacca
     6181 aagatcggca aagtcatcta cggcatccat acgggtctga aagagcacct gctgaaaccg
     6241 gtcgcgaggc cgacacagcg ctcatgacgt ttacggacat tctccaatgg tgccgccagc
     6301 atcacgccgt agtgcgcggc gtctatcgcg ggggtgctat ttccatttcg cacgaagata
     6361 aagagcttcc cgccgagatt ccggcgttcg atgacatttt tcactggggg atcgatatgc
     6421 cggaattaaa gcactacgtg agcggatcgg atttcgagcg catggtaaca ggaaagctga
     6481 caatcgatgg attcaaaagt tcgctgcgtg gacgagagta gactcggttc gtgatctggc
     6541 taaaaatgct tcgagctcag aaatgaccag cctgtgagct ggccatttct gagtcgatcg
     6601 actgctaggc aggtaagtta ggatttacca cttctggcga gttccatttt aaatggcgct
     6661 ccaaaaagtc cgtcaccagc ggccatgaca gatccgcagt atgcgcattg tatccgggcg
     6721 ccccaggatc cacataaccg tggccgccaa acggaaagaa gtgccactcc aaagattggc
     6781 gattttcttt gaacgcctgc cacatttgct cctgaaccgg caaaaccgtc gtgacatcgt
     6841 ccgcgccata cagaataacc gatgggcagc ggattttccg tgcgcttgct gatggatgaa
     6901 ccggtctcag cggcgaatcc ggctccgctc gcacagtcgg atagtaaccg acgaacgcac
     6961 gcacttcagg cgttgccgcg acaaaatgga tcgcgatgcg cccgcccatg cagtagccgc
     7021 ataccgccac ccgatcttta ttcacgttgg gttgctttag caaaaatcgc cagccttcgg
     7081 tgagcactcg ggtaaattcc gggtccggcg ttttggcttg aatttgcgat ccgctgacga
     7141 tgtgagtcgg ttcaggatag cccagctgct cgaatacgtt gatcgcaaag gtggtataac
     7201 cgagccgcgc gaactttcgc gtctccgttt taatgtactc gccaagcccg ccctttggat
     7261 ggatcaggaa cagcgccgga ccggcttcct gccgctccgg ataggacaga aatccggcga
     7321 cgccatgttc cttcgcgctg aaggctcttt cgatgatgcg cattaaaaat ctcctcgagt
     7381 agtttcgcgc aaactagcac gatcacttcc gctgggtaaa gagcttcgcg ggcctgaaca
     7441 ctattggcct gcggctgcgc aaaatccgag atttcgagaa aacgctcgtc ggcgaaggcc
     7501 gaatttctcg taagttacgg tcaggtctga accttatact tcctggaata ggaattgctc
     7561 ccccgctacg caaccgttca ccacgaccga gagttattca tctcatgcaa aacgatcgcg
     7621 cgccaactcc tatcccgcgg cgagagaaca acgaaaagaa aggcggactc atggctctcc
     7681 cggcgacgcc ttccttctcg ctcgcggagg ccaattccat cgttgagata gaggtccaag
     7741 ccgatcagct tctcggctac atcgataaac tgaccactct tgcagatcgg gcttgcacca
     7801 ccgcgatccg gcagagccaa acagcgcatc tgatagacgc gagccggcac ggtgaaatca
     7861 acgacctgcg caagaagctc gaccaacaga atgaaaagct gcatgaacag gaaattgcca
     7921 tcgttcgtct gcagcacgaa tcaaaagcgc agatcgctgc agtggaagcg cagttgcgcc
     7981 agaaccacct tcaaccgcgt cacgaaccgg agcagcatct tctgcgttac gaaaatgctc
     8041 agccggtaaa gcgcctgaac gattcaccgg agatcgcagc caccaaagtt gaaccggatc
     8101 atactcgatt ccatacggca aacgatccca cgattgcgga gttcaagctg cagatcgcca
     8161 atcgcgatga aaccatactg gccaagacta atgcactgaa agcaatcgag gaggagtatc
     8221 gcgcgagaat tgcgcatctc gagcagcgct tacaagaaac aacggcatta ttagaaaaac
     8281 acgcagagga gttaaacgaa aaagatcgca ttattgaagc cacggcctgc aaggaggcgg
     8341 agatgggaaa tttaatcaaa caactctccg ccgagtgcac cgccctcagt gaagagctgc
     8401 aaaaaagtga tcccgcaact gcgcgcggcg aggccaagcc aaacccaacc gtgaatgaaa
     8461 gcactctctg gcgccgcgtg ctcggccgtt tacaggaaga gccgcaataa caaactgcaa
     8521 cgcagccggc cgctgaattt gccactgcga tttaggattg agctgaggaa gccggcgagc
     8581 cctatcgatt ctaagctaag caaccttttt ctcggttgca gccttcgagc tttcttcgag
     8641 aatcatcatc accgccgcct catcatcgct taagccatgc tgcgcctggc gcgcagcagg
     8701 cacagggcgg ctcaggtttt ccggcaaact gccgtcgaag tacgctttta ccgttgccgg
     8761 atgaatgtaa cacttctgac aaactgccct ggtatttccc agctttcgcg ccaccgcttc
     8821 gacggcttga acgacatttc gtttagcctg gctgtgtgac gcaaacggtg gcaattcgcc
     8881 gagcgcgcgc gccgccagca cggtacctgc ccacgtacga aaatccttag ccgtaagatc
     8941 gacccccgtc gcttctcgga gatactgatt gacatcgctc gaattcacaa tgcgcggggt
     9001 gccgtcctcc tctaagtatt gaaataattc ttgcccgggg aggtcctggc aatgccgcac
     9061 gatttgcgcc aagcgatgat cgttgagatc cacatctcgg acaatgccgc tcttaccgcg
     9121 aaacctaaaa tggatcttcg agcccctaac ctttacatgc ttgttgcgca gtgtggtcaa
     9181 cccaaaggat tggttttgtc gcacatattc atcgttgccg atccgaatgc aagtggtttc
     9241 gagcaacctg acgatggtcg ccatcacttt cgctcgcggc agccccggca gagacaagtc
     9301 cttggccact tttctgcgta acttcgggag gactcggcca aactccatca ggtgatcgta
     9361 tttggtctga tcacgccact cacgccaggc gtgatgatat cgatactgtt ttcgtcctcg
     9421 agcgtcccta cccgtcgctt gcaaatggct gtttgagaac ggactgatcc agacatcttt
     9481 ccatgcagga ggaatcgcga gcgctctgat tcggcgtaac tcgccagcgt ttcgtatcgg
     9541 cacacctctc ggactaaaga acaaaaaatt tttcccatgc ctgcgtcggg taatcccttt
     9601 gatgctgtcc atgacgtagc ggagtccgcg cgacttgacg gcgcgaatcg gcgctacctt
     9661 cacaaccttg ttgcgttgat gcctcacttg ctttccgacc actgaccaga cgcccagcaa
     9721 atgatagacc actgtccgac agcaactggt ttcagagcat catcgcacgc tgtctgtttc
     9781 tatatcggac gccgaacgaa ttttaacgtc agtaaattgc cgcttctgta ataaaaagcg
     9841 gacagctggc gctgcacaag acaataagcg ctagatcaaa aaaggcctct cgctgcaaca
     9901 agaggccttg aaaaagatcg gcgacaacga tctcagacaa acaaaggcgc taacacgaga
     9961 gtgatggtgc ttagcaattt gatcagcacg tggaggctcg gcccggccgt gtctttgaaa
    10021 ggatcgccaa ccgtatcacc cacgacagcc gccttgtgag gatcggagcc tttgccgcca
    10081 aatgcgccgg tctcgatgaa ttttttcgcg ttgtcccagg cgccgccgct gttattcaga
    10141 aaggttgcca ttagaatgcc ggcgatcgtt ccgaccatta acaaagccgc caccgcttcg
    10201 gccccgacac cgaacaggcg aaagatcaca cccaccgcca ctggggtgcc cacggcgatg
    10261 gctccgggca gtaccatttc cttgagtgca ccgacggtca cgatatcgac gcagcgaccg
    10321 taatccggct tggccgtgcc ttgcagaatc ccaggatttt ctttgaattg cctgcgcacc
    10381 tcgttgatta cgtagtatgc ggccttgccg acggcgcgaa tcgctaacgc ggaaaagaga
    10441 aaaaccagcg ttgctcccaa caagcctccg acaaagactt ccggcctggc aatgttgacc
    10501 gcctcgagct tggcgccgta gtgcgccacc tcgtcgaggt acgcagagaa aaggagaaac
    10561 gccgcgagcg ccgcggagcc gatcgcatag cctttggtga gggccttcgt cgtgttgccg
    10621 acggcatcga gccgatcggt tttctgccgc acttcttcag gctggccgct catctcgatg
    10681 atgccacccg cattgtcggt gatcggcccg aaggtgtcca tcgccagaat ataagcggcg
    10741 gttccgagca tgcccatggt cgcaactgcg gtgccgaaca atccggcatg aggcacaccg
    10801 ctcatgccac ctagataata ggaagcgata atggcgatcg agataacgat caccggaatc
    10861 gcggtgcatt caaggccaac tgcaacaccc gcgataatat tggtcgccgg cccggtctgc
    10921 gatgcttcgg cgatgctttt caccggacga tatttcgctt cggtgtagta ctgcgtgata
    10981 tagacgaatg cctgcgccat caatatgccg atgacgccgc accagaaaaa atagagccac
    11041 gccgatgtcg cttccgggtg cgtccccggg tcaaccgcaa gcagccactt cgcggaaacc
    11101 aaaaacccgg cgatcgccag cgcgctggtc acataatagc ctcgattgag cgctgccatt
    11161 ggatcttctg tttccttgca tttgaccacc atgacgccga cgatggacgc aagcaatcca
    11221 aaagcccgcg cgaccagcgg aaacatgatt cctttcaacc cgaagtacgg gaacaaaccg
    11281 acgccgagaa tcatggcgcc gatattttcc gcggccgtcg attcgaacaa atcggcgcca
    11341 cgaccggcgc agtcgcccac gttgtcgccg acaaggtctg cgatgacggc ggggttgcgc
    11401 gggtcgtctt ctggaattcc ggcttcgact ttaccgacca aatccgctcc gacatccgcc
    11461 gccttcgtgt agattccacc ccccagctgc gcgaacagag cgacgaagga agcgccgaac
    11521 ccatagccga cgatggtgaa cggaatcttt tgaggatcat gcccgagcgc ttccagcaag
    11581 gcgtacaatc caccaacgcc caagagactc atggccacga cgaacaagcc cgataccgcg
    11641 ccgccgcgga gcgctatctg caggccgtca ttgaggctgg agcggacggc ggaggcagtg
    11701 cgaatattgg aacgaatcga aacccacatg ccgacgtaac ccgcgatcac ggagcaggcc
    11761 gcgccgagta caaacgagag agtggtccaa aaagccagtt gaactggcgg cacagggtcg
    11821 gcgggactcg gagtgcgcgc ataagcgtaa agcacaaata tcaaggccgc gagcactgcg
    11881 gagaaactga tgatcgtgcg attctgtcgc gataaaaatg cctcggcgcc ttctttgatc
    11941 gcattggaga tttgttgcat ttgagccgtc ccggcatcgc gctggatcac gtgacgagcc
    12001 aaaaagaccg cgaaggccaa cgacagacag ctgatggcaa tcacaaacgg aatcattaat
    12061 cctcctcatc cctaatcgag taaattcatc ctggtttgcg ccacacatac gcgacaccga
    12121 tgctgagcag ataaagcagc aacatggggc cggccaaaag acactgatta atcacatcgg
    12181 gagtcggcgt caagaccgcc gcaatgataa agatgagcac aatggcatag cggaatgagc
    12241 gcagcatgaa gtggtaatcc cagacgccga tgcggacgag aaaaaaagta aacaccggta
    12301 gctcgaaggt gagcccaaag gcgcccacca tgcgaaagaa aaacgtgaaa taatcgccga
    12361 tccgaatcgc cgggctcacg cccatggttt cgtactgttg gatgaaatag ctaaatgcta
    12421 ctggtaaaac gaaccggtag cagaagctcg cgccggccac gaaaaacaac gtcgcgcaaa
    12481 atccgaaagg cacgacccat ttccgttcat gttgcgacag accgggcgcg acaaagcgcc
    12541 aaatctgata aaaaatgact gggcaggaaa aaagaatacc cgccgccagc gcgactttaa
    12601 tctcgacgaa aaacgcctcg gtcacactgg tgccgatcag attttgacca ggcttgaggt
    12661 tctgccgaag cggcgcgacg ataaaattaa aaatcggctc ggaaaaaaaa taagatccgc
    12721 ctgcggcaat gacgatggca agaaccgatt tgagcaagca ccagcggaga tcctcgagat
    12781 gctccgtcag agtcatttga gaatcgcgcg ccacagtccg gaatcagaac gaaaattgca
    12841 aaaaagggga agggttcaaa cccttcccct ttttggtttt aaaaacctat aaggt
//
LOCUS       DASULZ010000364         3418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_248005, whole genome shotgun sequence.
ACCESSION   DASULZ010000364 DASULZ010000000
VERSION     DASULZ010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248005"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VFY96_07530"
     CDS             complement(<1..399)
                     /locus_tag="VFY96_07530"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HEX5606348.1"
                     /translation="MSETSDSRGRNIRKVRPFLEELEAKPFWANLREHKLTAQRCNAC
                     GKLFSFPPQGSCPHCLSTDYTWTPLSGKGKVYSFVNYHRAWHPSYEGKTPYNVSLVDL
                     DEGPRLISNVIDCPVDQVTIGMPVEVVYEDN"
     gene            complement(396..1550)
                     /locus_tag="VFY96_07535"
     CDS             complement(396..1550)
                     /locus_tag="VFY96_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX5606349.1"
                     /translation="MPNVSGRYAVVGVAETKVGKRPDASTHSLHLECMKACLDDAGLK
                     ASDIDGFITNQPLNDAHRSYAVRMANMARIHPNYATDLALGGATPIAMVQNAVMAIEA
                     GMANTVLCVHARKRSTPDPSPGNPIRHGDEHWEEPWGHFSAVANHAFAAQRHMYEFGT
                     TSEDLAHVAVATRKHACMNNNATMRKPMTIADHQASRFICEPLRLFDCALESDGGGAV
                     IVTSAERARDFPKKPVAILGMGQHHPHFSLMDAPSLTTLGGKKSSELAYKMAGLSPKD
                     MDFAEIYDCFTITTMITLEDYGFCKKGEGKDFVKNGRIEVGGELPVNTHGGLLSQAHI
                     EGMLHVTEAVRQLRGNEVEPERQVKDAKVGIVSGHGGSLCMHASLLLGAL"
     gene            complement(1662..2660)
                     /locus_tag="VFY96_07540"
     CDS             complement(1662..2660)
                     /locus_tag="VFY96_07540"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606350.1"
                     /translation="MAKRLIVVVLLALIGAIGVAGAQDKALKKFRWGVTSLSASQWIP
                     WIAKEAKLYEKYGLEAELVLLRGSGQSAQAIIGNSILGAPCTLTTVMSADLSGADLVT
                     VAHTVSGVQSKLLVRPEIKKPEDLRGKRVAVSGFGSLGDFLERYIIKKYGLEPGRDVI
                     MLSVGNQPDRIQALINGVVDAADLSHPADVQAERKGFKILWDAKQEVSYPSMSIVTRR
                     KWVAEDRDSVMRMVKAHVEGIHYLKANKEFSMKVLSKYLKTNDRELLEGSYEIYRKDF
                     ISVPYPITQGLQPTYEYVAAQRPEIWNQKPEAFMDSSFIAELEKTGFIKALAQSSR"
     gene            complement(2772..>3418)
                     /locus_tag="VFY96_07545"
     CDS             complement(2772..>3418)
                     /locus_tag="VFY96_07545"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5606351.1"
                     /translation="PKEAVKELERCIKELGFVAVATSAHSAGRNLDHPDFYPFYAKAQ
                     ELGVPVCVHVGSGRPAAAADRFDNAFFVHATTHAFEQMIGVMCIVGGGILELYPKLKV
                     AFLEAGAGWVPYWMERLDEHYEYLEPTVPLMKKAPSEYMRSEQVYYSFEPDEHTLNFV
                     MDYVGEDRLVFASDYNHGDSKFPHTVKAVTERKNLTQSQLQKLMCDNAARLYNV"
BASE COUNT          711 a          967 c          959 g          781 t
ORIGIN      
        1 gttgtcttcg taaactactt ccaccggcat gccgatggtg acctgatcca ccgggcagtc
       61 gatcacgttg ctgatcaagc gcgggccctc gtcgagatcc accagcgaca cgttgtacgg
      121 tgttttccct tcgtacgaag gatgccaggc gcggtggtag tttacgaagg aataaacttt
      181 gcctttgccg ctgagcggcg tccaagtgta atcggtggac aagcaatgcg ggcaggagcc
      241 ctgcggtgga aagctaaaca atttcccgca ggcattacag cgctgtgccg tcagcttgtg
      301 ctcacgcaaa tttgcccaga agggcttcgc ttccaattcc tcgagaaacg gcctgacttt
      361 tctgatgttg cgtccacggc tgtcgctggt ctcgctcata acgcccccaa aagtaaactt
      421 gcatgcatgc acaaacttcc gccatggccg ctgacaatgc cgaccttggc gtctttcacc
      481 tggcgctccg gctcgacttc gttgccgcgc aactgacgca cggcttcagt aacatgcaac
      541 atgccctcga tgtgcgcctg cgatagcagg ccgccgtggg tattgaccgg taactcgccg
      601 ccgacctcaa tgcggccgtt tttgacgaag tccttgccct cgcccttttt acagaagccg
      661 taatcttcca acgtgatcat cgtcgtaatc gtgaagcaat cgtaaatctc ggcgaagtcc
      721 atgtccttag ggctgagccc cgccattttg tacgcgagct cggaggactt cttgccgccg
      781 agtgtggtga gactgggtgc gtccatcaag ctaaagtgcg ggtgatgctg acccatgccg
      841 agaatcgcca cgggtttttt ggggaagtcc cgcgcgcgct ccgcgctggt gacgatcacc
      901 gcgccaccgc cgtcagactc caaagcgcaa tcgaacaaac ggagcggctc gcagatgaaa
      961 cgcgaggctt ggtgatcggc gatagtcatc ggcttgcgca tggtcgcgtt gttgttcatg
     1021 caggcgtgct tacgggtggc caccgcgacg tgcgcgagat cttcgcttgt ggtgccgaac
     1081 tcatacatgt gccgctgtgc tgcgaaggca tggttcgcga ccgcggaaaa gtggccccag
     1141 ggctcttccc aatgttcgtc accgtggcga atggggttcc caggcgacgg atcaggcgtg
     1201 ctgcgcttgc gcgcatgcac gcatagaacg gtgttcgcca tgccggcttc aatggccatc
     1261 accgcattct gcaccatcgc gatcggcgtc gcgccgccga gggcgagatc ggtggcatag
     1321 tttggatgta tgcgcgccat attggccatg cgcaccgcat aactgcggtg cgcatcattc
     1381 agcggctgat tggtgataaa accgtcgatg tcggaggctt tgaggcccgc gtcatccaga
     1441 caagctttca tgcactccag atgaagggaa tgcgtcgatg cgtcaggccg ctttccgact
     1501 ttggtttcgg cgacgccaac gacggcgtaa cggccgctga cattaggcat aagaaatcct
     1561 ccataaaaat gggagtgttg gagtaatgga atgttggata gataagaccc accactccgg
     1621 cactccacta ctccattact ccaattggta agattttgaa actaacgcga ggattgagcg
     1681 agcgctttga tgaatccggt cttctcgagc tcggcgataa aactcgaatc catgaaagct
     1741 tccggcttct gattccagat ttccgggcgc tgcgcggcga catattcgta ggtgggctgc
     1801 aaaccctggg tgatcgggta gggaaccgag atgaaatcct tgcgataaat ttcataggag
     1861 ccttcgagca gctcgcggtc gttggttttg aggtatttgc tgagaacttt catgctgaac
     1921 tctttgttgg cctttaaata gtgaatccct tcgacgtgcg ctttgaccat gcgcatgacg
     1981 gaatcgcgat cttcggcaac ccatttgcgc cgggtaacga tcgacatcga cggataggaa
     2041 acctcctgct ttgcgtccca caagattttg aaccctttgc gctcagcttg cacgtcggca
     2101 ggatgggata aatcggcagc atcgaccacg ccattgatga gagcttgaat tctatccggt
     2161 tggttgccga cggagagcat aatgacgtcg cggccgggct cgagaccgta tttttttatg
     2221 atgtaacgct cgagaaaatc gcccagcgag ccaaaaccgg acaccgcgac tcttttgccg
     2281 cgcagatctt ccggtttttt gatttcgggt cggaccaaca acttgctttg cactcccgaa
     2341 accgtatgcg ccacggtgac tagatcggcg ccgctcagat ccgcgctcat caccgtggtc
     2401 aaagtgcagg gcgcgccgag aatgctattg ccgatgatcg cctgcgccga ttgccccgag
     2461 ccgcgcagca gcaccaactc ggcctccaaa ccgtacttct cgtagagctt ggcttccttg
     2521 gcgatccacg gaatccactg cgaggcggag agcgaggtca cgccccagcg aaatttcttg
     2581 agcgccttat cctgcgcgcc ggcgacccct atcgctccga tcagagctag cagaaccact
     2641 acaattaatc gctttgccat attcttgttc ctgttttctc ggtgctcagc ggaacagaga
     2701 agttttagat gcttcgcttt gctcaacatg agagaaagcg ttgtcgtcct acagaaaccg
     2761 agaatgtcac tctaaacgtt atatagccgc gcggcgttgt cgcacatcaa tttttgcaac
     2821 tgactctgcg tcaggttctt ccgctcggtg accgctttca ccgtatgagg aaacttgctg
     2881 tcgccgtgat tgtaatcgga cgcaaatacc aaacgatctt cgccgacgta gtccatgaca
     2941 aagtttaagg tgtgctcgtc gggctcgaag gagtagtaca cctgctcgct gcgcatgtac
     3001 tcgctcggcg ccttcttcat cagcggcacg gtgggctcga ggtattcgta gtgttcgtcc
     3061 aaacgctcca tccagtaggg tacccagccg gcgccggctt ccaagaaagc gaccttgagc
     3121 ttcggataga gctcgagaat gccgccgccg acgatgcaca tgacgccgat catctgctcg
     3181 aaagcatgcg tcgtggcatg gacaaaaaat gcgttgtcga accgatcggc tgcggcggcg
     3241 ggtctgccgg aaccgacgtg cacgcagacg gggactccca gttcctgagc cttggcgtaa
     3301 aacggataaa agtcgggatg atcgaggttg cgccctgctg aatgcgcgga ggtggcgacg
     3361 gcgacaaacc ccagttcttt gatgcagcgc tcgagttctt tgacagcttc tttcggct
//
LOCUS       DASULZ010000365         2408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_654421, whole genome shotgun sequence.
ACCESSION   DASULZ010000365 DASULZ010000000
VERSION     DASULZ010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654421"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VFY96_07550"
     CDS             <1..402
                     /locus_tag="VFY96_07550"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011426912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5606352.1"
                     /translation="FTGDNRLRVCESAMAAAVAARQGVRELNRTRSAENLPVTDMYLA
                     LHVGTVFYGNIGSKERLDFTVIGPAVNEVSRIAAMCRSVDQPLLVSSAFAAAVGDFRS
                     HLVSVGRFALRGIAQPQELYTLDADLQIRTK"
     gene            536..1321
                     /locus_tag="VFY96_07555"
     CDS             536..1321
                     /locus_tag="VFY96_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606353.1"
                     /translation="MKPSLLIATLSRAACFAALLFFAIFPAARAQDKIPPEVINETAS
                     HGTALVLVGLSVPWEMESKLDGDGVRAQRTAIASIQNDLLTQLEGRNYKIVRRYQEIP
                     GIALEVGADALAELARLPIVTNVLLDRLPGAAEHRANFASARTEAPSARAADPVAEKV
                     PSQLFTRAASNGTVLVLAGLRTPWQREELLSESLLVLQRAAIQDAQSYILAELAGTTF
                     RVTRLYRSIPGIALRVGIDALKVLEKSPAITNVVQDRPAQSAR"
     gene            complement(1343..1774)
                     /locus_tag="VFY96_07560"
     CDS             complement(1343..1774)
                     /locus_tag="VFY96_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6677 family protein"
                     /protein_id="HEX5606354.1"
                     /translation="MPASSTPRVVLLCCVLLPGLGQIYNRESKKGLIIFACAVSLGVL
                     GLCFSGFNRLAMLLALGLLWVSAATDAYQMAKNASRPAEFYYRKSFIVAMLLLVGPLA
                     LPLLWKSTNFSRTARWLWTIVVVSVALLFIATPYLLKGIVV"
     gene            complement(1921..>2408)
                     /locus_tag="VFY96_07565"
     CDS             complement(1921..>2408)
                     /locus_tag="VFY96_07565"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5606355.1"
                     /translation="HASASLNPAMHLTNAYYIAHHNSAAVEILRSRVFKDFPKLKIII
                     PHGGGAIPYQWNRQRGMHVKEGLEPFEEAARRVYWDMAIYDKESMELLIKRVGADNVL
                     FATEMFGAVNAIDPKTGRNFEDLVPIFQDIEWLTDDDRYKITEGNAKKVFSHMLKQTK
                     G"
BASE COUNT          547 a          699 c          648 g          514 t
ORIGIN      
        1 ttcaccggtg ataatcgact gcgtgtctgc gaatccgcca tggcggctgc tgtcgccgct
       61 cgccaaggcg tccgcgagct gaaccgcacc cgttcagcag aaaatctgcc cgttacggac
      121 atgtacctcg cgcttcacgt cggaactgtc ttttatggca acattggcag caaggaacgg
      181 cttgatttca ctgtcatcgg ccctgctgtg aatgaagtaa gccggattgc cgcgatgtgc
      241 cgttccgtcg atcaaccatt gcttgtctct tcggcgttcg cagccgcggt tggcgacttc
      301 cgaagccacc tcgtgtcggt cggccgcttc gctttgcgcg gcatcgccca gccccaggag
      361 ctctataccc tcgatgccga tctacagatt cgcactaagt gagcctggtg aatcgagagc
      421 cggacggacg cacagaggaa tctggacagc gacaccgatg cgggtataag atccctcccg
      481 agtaggcaac tcgcttttct tcccatggtc gatctagcct cggacgggag gaatcatgaa
      541 gccatcgctg ctaattgcga ctctatctcg agccgcttgt ttcgctgccc tgctattctt
      601 cgcgatcttc cctgcggcac gagcccaaga caaaattcct cctgaagtta tcaacgagac
      661 agcctcccac ggtacggcgt tggtgctcgt cggattgagc gtgccatggg aaatggagtc
      721 aaagctcgat ggcgatggcg tgcgcgcgca acgcaccgct attgcctcga ttcagaacga
      781 tctactgacc caattggaag gaagaaatta taaaattgtg cgccgatatc aggaaattcc
      841 cggcatcgct ttggaagtcg gcgccgatgc attggccgaa ctagcgcggc tccccatcgt
      901 gaccaacgtg ctgctcgacc gcctgcctgg cgcggcggaa catcgagcca atttcgcttc
      961 ggctcgaacc gaagcgccga gcgctcgcgc cgccgacccg gtcgcggaga aagtgccttc
     1021 gcaactgttc acgcgggcag ccagcaacgg taccgttctt gtgctcgcgg ggttacgcac
     1081 accttggcag cgcgaggagc ttttgagcga atcgcttctc gttctgcaac gagcggcgat
     1141 tcaagacgcg cagagttata tcctggcgga gctcgccggc acaaccttta gagttacccg
     1201 actctacaga agtattcctg ggatcgccct gcgcgtcggc atcgatgcgt tgaaagtatt
     1261 ggagaagtcg cctgccatca ccaatgtggt acaggatcga cccgcgcaat ctgcgaggta
     1321 agagcgtgct tacaattcca aactaaacaa caattccctt caacagatag ggtgtcgcga
     1381 taaacaacag cgcgacactg accacgacaa tcgtccagag ccagcgagcg gtccgggaaa
     1441 aattcgtgct tttccagagg agcggcagcg cgagtgggcc gacgagcaac aacatcgcga
     1501 cgatgaagct tttgcgatag taaaattccg cgggccggct agcgttcttg gccatctggt
     1561 aggcatcagt cgcagcgctc acccaaagta aacctaaagc gagcagcatg gccaatcggt
     1621 tgaacccgga aaaacaaaga cccagcactc ccaggcttac cgcacaggcg aaaatgatca
     1681 ggcctttttt gctctcccga ttatatatct gcccgaggcc gggcaagagc acgcaacaga
     1741 gcagaacgac ccgaggggtc gagctagcgg gcatcgaact tagctccacc acggcgcaat
     1801 gagatgaccg cggtaaagac agagcggcgt caatgtgagt tttcacacag actgccgttc
     1861 aaaacggcag tctaaacttg cgataatcga ctcagtctcg atcgtgtccg atcgataatc
     1921 ttagcctttg gtctgcttca acatgtgcga gaaaactttt ttggcattgc cctcggtgat
     1981 tttgtaacga tcgtcatcgg tcagccactc gatatcctga aaaatcggca ccagatcctc
     2041 gaagtttcga ccggtcttgg gatcgattgc attcacggcc ccaaacatct ccgtggcaaa
     2101 caacacattg tccgcgccga cgcgtttgat taaaagctcc atcgattcct tgtcataaat
     2161 cgccatgtcc caataaaccc gccgcgccgc ttcttcgaac ggctcgagcc cttctttcac
     2221 atgcatgccg cgctggcgat tccattgata gggaatggcg ccgccgccgt gcggaataat
     2281 gattttcagt tttggaaaat ctttgaacac gcgcgatcgc aaaatctcta cggcggcgga
     2341 attgtgatgc gcgatatagt acgcattggt gagatgcatc gccggattga gcgatgcgct
     2401 ggcgtgca
//
LOCUS       DASULZ010000366         4427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1059412, whole genome shotgun sequence.
ACCESSION   DASULZ010000366 DASULZ010000000
VERSION     DASULZ010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059412"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..959
                     /locus_tag="VFY96_07570"
     CDS             72..959
                     /locus_tag="VFY96_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017728756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5606356.1"
                     /translation="MDNRRYDYSPIMRRPRIEWPDHGRIALWVAPNIEYFHFDMPIRG
                     SGSNHTPDVPGYTLRDYGSRVGVFRIMEVLDKYGIRASVLLNAEVCERHPPIIEEGNK
                     RNWEWLGHGMTNSISMIDYPPEEERSVIHKVKEIITEATGKAPKGWLGPGLGETFDTP
                     DHLAAEGFEYVCDWGNDEQPTPMRVKSGRMIVVPYELGVNDIRVFIRETHTPEQYYRL
                     VCDHFDTLYKDSESGGRVLCLPLHPFVIGMPFRIKYLDKALDYMCSHEGVWRTTGWDI
                     ADWYYQHYYKDPGRIEVKE"
     gene            complement(1035..1748)
                     /locus_tag="VFY96_07575"
     CDS             complement(1035..1748)
                     /locus_tag="VFY96_07575"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX5606357.1"
                     /translation="MKKFFALAGVLLLLTPSGWAQQERNRPRTSAITVTGEALISAEP
                     DQAQLEIGVVTQARTAPDASKENAERLTRVLSEIKKLLGKTDEVKTSGYSLTPAYRYP
                     QGGKPEIVGYNASNTVRIKTTNLDLVGRLIDSAMQAGANNVNRLVFTLKDEQSAQLEA
                     LRAASLKARTKAEAIATTLGLKVVKITSVAEGERMIQPIVRQITALRAEAAPAQTPVE
                     PGTVDVRSTVSMTVEVTQQ"
     gene            1929..3245
                     /locus_tag="VFY96_07580"
     CDS             1929..3245
                     /locus_tag="VFY96_07580"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5606358.1"
                     /translation="MEGAARFEQQIKPRIFYGWYIVAVGFLANVASSFALASTLSIFL
                     KPLTAELGISRGVFSLLRSGEGMIGAGLAPLLGTLVDRYGGRWLMVIGTIIVGIGYFT
                     LSHVENFAQFAGIRLTLVTLGDAMMGYMVINVVISQWFVRRRGRALAFSSMGVGFAKV
                     CMPIIAASLLVAFGWRQTWFIFGITTMALLVLPALFVIRRTPEEMGLYPDGAAEPTLA
                     ASSSPAKKAASDLLRPFSEAVWTRAEAVRTSAFWLLVITFGISSVGVTGLNLHVYSYV
                     TDLGHSAVVAASVMSVIASMQLASPLAWGLLAERIDPRYAAMLRFIVQGVGLGIAIAT
                     GNLLCLYGGFFLYGIGLGGNMVLPDILWANYFGRHSLGKIRGMGLLISQVLAAAGPPF
                     FGFLFDITGGYGLSFALFGCVLMTSAGLSLMLRAPAKKEWRDGVLE"
     gene            3357..4352
                     /locus_tag="VFY96_07585"
     CDS             3357..4352
                     /locus_tag="VFY96_07585"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5606359.1"
                     /translation="MIIDVHNHIVAGEQLQTFQAGLINSAGFHRPRGTVVTEKHIQEA
                     KWHGHRHSEVLDEVGTDFAFISPRPYTMMHSAKPEKIVHWYCQAVNDALALQVKMEPK
                     KFRAIGGLPQNSGVPVTNTFEEIDRCINDLGFVGVMINPDPGEGDGQTPAMGNEYWYP
                     LYEKMVEMDIPGLVHAASCKNPRESFHGHFITEESIAILSLVDSKVFQDFPKLKLIIS
                     HGGGSVPYQVGRWRSQLRGPNDTENFDQRLKRLYFDTALYNVESLKLLFRIVGPDRCL
                     FGTERPGAGSKTDPRTGKWYDDVRPNIESISWLTTEDKAKIFERNAVHVFSRFKP"
BASE COUNT          942 a         1164 c         1255 g         1066 t
ORIGIN      
        1 tatttagcac ctagaatagg ctacatggcc ggggacaaat ttgtaatcag tttccggagg
       61 taaatagaga aatggataac cgccgctacg actactcgcc gatcatgcgc cgaccaagaa
      121 tcgaatggcc cgatcatggc cgcatcgctt tgtgggtggc accgaatatt gaatacttcc
      181 atttcgatat gccgatccgc ggctcaggca gtaatcatac gcccgatgtt ccgggctaca
      241 cgctgcgcga ttacggttcg cgggtcggag tattccgaat catggaagtg ctggataagt
      301 acggcattcg cgcgagtgtt ctattgaacg ccgaagtgtg cgaacggcat ccgccaatta
      361 tagaggaagg caataagcga aattgggaat ggctcggaca cggcatgacc aacagcattt
      421 cgatgatcga ctatccgccg gaagaagaac gttcggtcat ccataaagtt aaggaaataa
      481 taacagaggc gaccggtaag gctccgaagg gttggttggg tcccggacta ggtgagactt
      541 tcgacacgcc ggatcatttg gcggcggaag gtttcgagta tgtgtgcgat tggggcaatg
      601 atgaacagcc gacgcccatg cgggtcaaaa gcgggcgaat gatcgttgtc ccatatgaac
      661 tcggtgtcaa cgatattcgg gtttttatcc gtgaaacgca cacgcccgag cagtattacc
      721 gattggtttg cgatcatttc gacactttgt acaaggacag tgagtcaggc gggcgagtgc
      781 tttgcttgcc gcttcatcca ttcgtcatcg gcatgccgtt tcgcatcaag tatctggaca
      841 aagcactcga ttatatgtgc tcccacgaag gagtctggcg caccacggga tgggacatcg
      901 cggactggta ctatcaacat tattacaaag atcctgggag aattgaggtg aaggagtaag
      961 tggagtacag ggagtgatgg actgtgggtt aggggaagcc agtactcgac cactgcacca
     1021 ctccgttcct ccagctattg ctgtgttact tcaaccgtca tcgacaccgt cgagcgaacg
     1081 tctaccgtgc cgggctcaac cggggtctgc gccggtgcgg cttctgctcg cagcgccgtg
     1141 atctgacgaa caatcggctg gatcattcgc tcaccctctg cgactgaagt gatcttcaca
     1201 actttgagac cgagcgtcgt ggcgatcgct tcggctttgg ttctcgcctt gagcgaagca
     1261 gcgcgcaagg cttcgagctg agctgattgc tcatctttca acgtgaaaac cagacgattc
     1321 acattgtttg ctccagcctg catcgcgcta tcgatcaagc ggccgacgag atccaaattg
     1381 gtggtcttga tccgaacagt gttgctggcg ttatagccaa caatttcggg cttgccgccc
     1441 tgaggatacc ggtacgcggg cgtcaacgaa tatccgctgg ttttcacttc atcggtttta
     1501 cccaatagtt ttttgatttc cgacaatact cgagtaagtc gttcggcatt ttctttggaa
     1561 gcgtccgggg cggtacgggc ctgtgtcacc acgccgatct cgagttgagc ctgatccggc
     1621 tcggccgaga tgagcgcttc acctgtgaca gttattgcgg aagtgcgcgg acggttacgc
     1681 tcttgctgcg cccacccgga tggcgtcaac aaaagcaaga caccggctag cgcaaaaaat
     1741 ttcttcattg cctgagaacc tttcttctcc gctcagaaga ctacaaaaac ttgaagtgag
     1801 acccaggtag atgcctcgcc cgaacaactt cattcgatta gacgtcaatg ttgccgaaag
     1861 gttcatttca ccggtcgatg ttattgttcg aattgcgcga acacgaagat acctgctaaa
     1921 gcgaaggggt ggaaggtgct gcccgctttg agcaacaaat caagccgcga attttttacg
     1981 gctggtacat tgtcgctgtc ggatttctcg ccaacgttgc ttcctctttc gcgctggcga
     2041 gcacgctgag catttttctt aaacctctca cggccgagtt gggcatctcg cgcggggtct
     2101 tttccttgct acgctcgggc gaaggcatga tcggtgccgg acttgcaccg ctcctcggca
     2161 cgcttgttga tcgctacggc gggcgctggt tgatggtcat tggcacaatc atcgtcggca
     2221 tcggttattt cactctcagt cacgtggaaa atttcgcgca gttcgccggc attcgcctga
     2281 ccttagtcac tttgggcgac gccatgatgg gctacatggt gatcaacgtc gtgatctccc
     2341 agtggttcgt gcgccggcgc gggcgggcac tggcgttctc cagcatgggc gtgggttttg
     2401 ccaaggtctg tatgccgatc attgcggcat ctctgctcgt ggccttcggc tggcgccaaa
     2461 cctggttcat cttcggcatc acgaccatgg ctcttttggt gctaccagcg ctgttcgtga
     2521 ttcggcgcac gcccgaagaa atgggactct atcctgacgg cgctgccgag cccacgcttg
     2581 cagcatcatc gagcccggcg aaaaaagcag cgagcgattt attgcggcca ttctcagagg
     2641 cggtttggac gcgcgctgag gcggtgcgca cgagcgcatt ctggctgttg gtgatcacct
     2701 tcgggatttc cagcgtcggc gtgaccggat tgaacttgca cgtttattcc tacgtgaccg
     2761 atctcggaca ttccgcagtg gtggcagcat cggtgatgag cgtgatcgcc tccatgcaat
     2821 tggcatcgcc gctggcctgg ggccttctcg ccgagcggat cgatccgcgc tacgcggcga
     2881 tgttgcgctt catcgtgcaa ggcgtcggct tggggattgc gattgcgacg ggtaatttgt
     2941 tgtgcctgta cggcggattt tttctctatg gcatcggcct tggcggcaac atggtattgc
     3001 ctgacattct ctgggcgaac tattttggcc ggcactcgct cggcaagatt cgcggcatgg
     3061 gattgctgat ttcgcaggtg cttgctgccg ctggaccgcc gtttttcggt tttctcttcg
     3121 acatcaccgg cggatacgga ctgtccttcg cgctgttcgg ctgtgtttta atgacttcgg
     3181 cgggattaag cctcatgttg cgcgctccgg cgaagaagga gtggcgtgat ggagtattgg
     3241 agtaatgaag tattggaaaa cccatcactg cagcacttca gtactccgat gccacggctt
     3301 gctgctggtt ggggctctct gataggaaaa gtcaattccc ttgaaaggat ctcgtgatga
     3361 ttatcgatgt tcacaatcac atcgttgccg gtgagcaact gcagacattc caagccggat
     3421 tgatcaacag cgcaggcttt catcgcccac gcgggacggt ggtgacagaa aagcatattc
     3481 aagaagccaa atggcatggc catcggcact ccgaagtcct cgatgaagtc gggacagatt
     3541 ttgcgttcat ctcgccgcga ccctacacga tgatgcattc ggccaaaccg gaaaaaatcg
     3601 ttcattggta ttgccaagcg gtgaacgacg ctttggcctt gcaggttaaa atggagccga
     3661 agaaatttcg cgccatcggc gggctgccgc aaaacagtgg cgtccccgtg accaacacct
     3721 tcgaggaaat cgaccgttgc atcaatgatc tgggttttgt cggcgtgatg atcaatcccg
     3781 atccagggga aggcgatggt cagacgccgg caatgggcaa cgagtactgg tatccgcttt
     3841 acgaaaaaat ggtagagatg gatatcccgg gactcgtgca cgccgccagc tgtaaaaacc
     3901 cacgcgaatc ctttcacgga cattttatta ccgaggaaag catcgcgatt ttgtcgctgg
     3961 tcgattcaaa agtgtttcag gattttccca agctcaagct gattatctcg cacggcggcg
     4021 gctcggtgcc gtatcaagtc gggcgctggc gctcgcagtt gcgcggtccc aatgacacgg
     4081 aaaacttcga tcagcgattg aaacggcttt actttgatac ggcgctttac aatgtcgaat
     4141 cgctgaagct gctgtttcgc atcgtcggtc ccgaccgctg tctgttcggc acggaacgtc
     4201 cgggtgccgg ctccaagacc gatccccgca cgggaaaatg gtacgatgac gtgcggccca
     4261 acattgaaag catttcctgg ctgactaccg aggacaaagc caagatcttc gaacgcaacg
     4321 cggttcacgt gttctcgcgg ttcaagccat agcgtccgcg gcgcaatccc atcggatgat
     4381 tcacgtttgt agacccgcct cttctagttg aaaactgcgc ggtttac
//
LOCUS       DASULZ010000367         6310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_46542, whole genome shotgun sequence.
ACCESSION   DASULZ010000367 DASULZ010000000
VERSION     DASULZ010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6310
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46542"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /gene="rseP"
                     /locus_tag="VFY96_07590"
     CDS             complement(<1..500)
                     /gene="rseP"
                     /locus_tag="VFY96_07590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEX5606360.1"
                     /translation="MENLVYSIGAAVIGLGLLIVFHEFGHFLLAKLSGVGVLTFSVGF
                     GPKLWVRKKGETEYALSAFPLGGYVKMVGEDPDEEVRQEDVERSFAHKSLLKRIAIVV
                     AGPGFNLLLALVLLMGVFTFYGVPVMSTQVSGVESSSPAERAGILKGDRIIAIDGKPV
                     KEWEALS"
     gene            complement(522..1670)
                     /locus_tag="VFY96_07595"
     CDS             complement(522..1670)
                     /locus_tag="VFY96_07595"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX5606361.1"
                     /translation="MKKLALLGSTGSIGVSTLALVREFPEKFQIHGMVAGRNLPLLAK
                     QIREFSPACVAIQREEDVPRLRALLGRRKLEILHGESGASAVAAAAEVDVVLAAIVGG
                     AGLMPSFNAVLAGKEIALANKEALVMAGELFVSAAKKKRVRLLPVDSEHSAIFQCLQG
                     NDQADVDKVILTASGGPFLKTPLHRLEKVTVAEALKHPNWKMGQKITIDSATMMNKGL
                     EVIEARWEFDLEPSRIDVVIHPQSVVHSMVRYQDGSIIAQLGIADMRIPIAYALAFPH
                     RLKGSWEGLDLTRQKLLNFLPVEKKRFPALALAYHALREGGTMPAVLNAANEVAVAAF
                     LHRRIGFREIHRIIGNIVQKHNNHRAKAIGDILEVDRWAREKASSLIH"
     gene            complement(1667..2473)
                     /locus_tag="VFY96_07600"
     CDS             complement(1667..2473)
                     /locus_tag="VFY96_07600"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX5606362.1"
                     /translation="MDGNLSSRVMTAALALPLLVLLVGWSPPWLFAAVVFLVTVGALQ
                     EYFTIVFPNRSRDRRLGVVFGICLSGAIFLNEYVSSLIWLDMVVLIGFCIYVLSGGAL
                     VDRLNRLVISLLGAIYAGYFLPHWVLLFRWPGGRAWVFWVLAVIMSGDTMGYFIGRRF
                     GARKLAPEISPGKTVAGMWGYVVGAVLAGLLAAFALFESIHWLEITVLSVALSILGQM
                     GDLFESWIKRVSGVKDSGNLLPGHGGLLDRLDSLIFPAVFTTAYLQAFHR"
     gene            complement(2457..3233)
                     /locus_tag="VFY96_07605"
     CDS             complement(2457..3233)
                     /locus_tag="VFY96_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEX5606363.1"
                     /translation="MELQDLDKSRLPKHVAIIMDGNGRWAKLRGKNRIEGHRRGKTSV
                     RVIVEMSRKIGIRYLSLYAFSTENWFRPHDEVDALMGLLEHYLAAEQAKMMRYGIRLL
                     AIGDRSRLPANVLTILEKVIELTRENQRMTVILALSYSGRDEIVRMAKDLALQARDGK
                     IDAHIIDEQMVAAHLDTCGVPDPDLLIRTSGEMRISNFYLWQLAYSELYMTPTLWPDF
                     REPEYLTALLDYQRRRRRFGRTDEQIDDSTLRLGAGGRQS"
     gene            complement(3336..3893)
                     /gene="frr"
                     /locus_tag="VFY96_07610"
     CDS             complement(3336..3893)
                     /gene="frr"
                     /locus_tag="VFY96_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011230752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX5606364.1"
                     /translation="MNEAIFTQLQKEMDGSVSALRKELGKIRTGRASTGLLEHITVDY
                     YGATTPLIQLATLSAPEPRLLVIQPFDRGAMGEIEKAILKSDLGLTPSNDGKIIRIPI
                     PQLTEERRKELVKHVKKVAEEYRVSVRNHRRVAIEHVKDAEKKKEITADDSKHAHDRV
                     QKITDDFIGRIDKIVKSKEEEVMAV"
     gene            complement(3890..4621)
                     /gene="pyrH"
                     /locus_tag="VFY96_07615"
     CDS             complement(3890..4621)
                     /gene="pyrH"
                     /locus_tag="VFY96_07615"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX5606365.1"
                     /translation="MDNGAPKYKRIILKVTGEVFAGPLNFGIDGNTVKAFAQEIKEVK
                     ELGCELALVIGGGNIFRGAIASEIGMDRASADTMGMLATVINSLALQDALEKLGVSTR
                     VLSAIEMRQVAEPYIRRRATRHLEKGRVVIFAGGTGNPYFTTDTTASLRAMEVGAEVI
                     LKATKVDGVYDADPFKTEGARKFDELSYIEVLNRELKVMDSAAISLCMDNNLPIIVFN
                     LMEKGNIKRVVSGEPIGTLVSGGKR"
     gene            complement(4639..5277)
                     /gene="tsf"
                     /locus_tag="VFY96_07620"
     CDS             complement(4639..5277)
                     /gene="tsf"
                     /locus_tag="VFY96_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX5606366.1"
                     /translation="MNFRLRREFKVEISASAVKELRDKTGAGMMDCKKALGECSGDVQ
                     KAIDYLRQKGLAAAAKKADRVAADGAVGAYVHPGGKIGVLVEINCETDFVARTAEFQM
                     LVKDIAMQVAAANPHYVRREEVSPEHLEKEKTIYRQQALETGKPEKVVDKIVDGKIDR
                     FYSEVCLLEQAFIKDPDKKVADVVNDAIARLGENIQVRRFARYHLGEAVTKE"
     gene            complement(5249..6019)
                     /gene="rpsB"
                     /locus_tag="VFY96_07625"
     CDS             complement(5249..6019)
                     /gene="rpsB"
                     /locus_tag="VFY96_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX5606367.1"
                     /translation="MSDISMKQLLEAGVHFGHQTSRWNPKMKPYIFGARNGIHIIDLQ
                     QTVTMFKELEAVVRDLAASGGHVLFVGTKKQAQDAIREEAERCGMYHVHNRWLGGTLT
                     NFSTIRQSIERLRKLEEMENDPKIVEALTKKEMLGLKREKAKLEQALGGIKSMRKLPD
                     AIFVIDPKQEEIAVKEARKLGIPVISVIDTNCDPDMVDYKVPGNDDAIRAIRLFCAAV
                     ADAINEGRALYEQSLTKRKEEERADTPNVNEFSAAAGV"
BASE COUNT         1421 a         1850 c         1541 g         1498 t
ORIGIN      
        1 gacagcgctt cccattcttt gaccggcttt ccatcaatcg caataattcg atcgcctttg
       61 agaatgcccg ctcgctccgc cggcgagctt gattcgaccc cgctgacttg cgtcgacatt
      121 accggcacgc cgtaaaacgt aaacacaccc atgagcagca ccagggcaag cagcaagttg
      181 aatccagggc cggcaaccac gatggcgatc cgcttgagca ggctcttatg cgcaaacgat
      241 ctttccacat cttcctgacg cacctcttca tcgggatcct cgccaaccat tttgacgtat
      301 ccgccgagcg gaaacgcgct caatgcgtat tcagtctcac ctttttttcg cacccagagc
      361 ttaggaccga agccaaccga gaaagtcaga acgccgactc cggaaagctt ggcgagcaaa
      421 aaatgaccaa actcatggaa gacgatcagc aatcctagtc caatcaccgc tgcaccgatt
      481 gaataaacca agttttccat ccacatctcc ttgcccgtga tttagtgaat cagcgacgac
      541 gctttttccc tcgcccagcg atccacttcc agaatgtcgc caatggcctt cgccctgtgg
      601 ttattatgtt tctgcacgat attcccaatt atacgatgta tttcgcgaaa gcctatgcgc
      661 cggtgcaaga atgccgctac cgcgacttca tttgccgcat tgagcactgc cggcattgtg
      721 ccaccctcgc gcagggcatg gtaggccaat gccaatgcgg gaaagcgctt cttttcaact
      781 ggaagaaagt tcagcagttt ttgccgagta aggtcgagcc cctcccagct ccccttgagg
      841 cgatggggaa atgccagcgc ataggctatg ggaatgcgca tgtccgcgat gccgagttgg
      901 gcaatgatcg aaccatcctg ataacgcacc atcgagtgca ccacgctctg cggatgaatg
      961 acgacgtcga tacggctagg ctccagatcg aattcccagc gagcctcgat cacctcgagc
     1021 cccttattca tcatggttgc ggaatcgatg gtgatctttt ggcccatttt ccagttcgga
     1081 tgtttcagtg cctctgcgac tgtgactttc tctagacgat ggagcggcgt tttcaaaaaa
     1141 ggaccgccgg acgccgtcaa aatgacctta tcaacgtcgg cctgatcatt cccttgcaag
     1201 cactgaaaga tcgcgctgtg ctcgctgtcc acgggcaaca ggcgaacgcg tttctttttt
     1261 gccgcgctga cgaagagctc acctgccata accagcgcct ctttattcgc cagagcaatt
     1321 tcttttcctg caagtacggc gttgaaagac ggcattaatc cggcgccgcc gacaatggct
     1381 gcgagcacga cgtcaacctc cgccgccgct gcgacagcgg atgcgcccga ttcgccgtga
     1441 agaatttcaa gctttcggcg accgagcaag gctcttaatc gcggcacatc ctcttcgcgc
     1501 tgaatcgcca cgcaggcagg tgaaaactca cggatttgtt tcgccagcag gggcaaattt
     1561 cggcccgcca ccatgccgtg aatctgaaat ttttcgggaa attcccgaac cagcgcaagg
     1621 gtgctgacac cgatggaccc cgtggaaccc agaagcgcaa gctttttcac cgatggaatg
     1681 cctgcagata cgcggtagta aagacggctg gaaagatcaa gctatcgagc cggtccagca
     1741 ggccgccatg gcccggcaac aggttacctg aatctttgac tccagagacc cgcttaatcc
     1801 atgactcgaa aagatcgccc atttgcccga gaatactgag cgctacagac aacactgtaa
     1861 tttcaagcca atggatcgac tcgaacagcg caaaagcggc aagcaagccg gcgaggacgg
     1921 cgcccacaac atagccccac atcccggcaa ccgtctttcc cgggctaatc tcaggtgcaa
     1981 gcttgcgagc accaaaacgt ctgccaataa aataacccat cgtatcccca ctcatgataa
     2041 ccgctaagac ccagaatacc caggcgcgac ctccgggcca gcgaaataac aagacccaat
     2101 ggggcaggaa gtatcccgca taaattgccc cgagcaaact gatcacgagc cggttcaacc
     2161 gatcaactag ggctcccccg ctcaagacgt agatgcagaa cccaatcaag accaccatat
     2221 caagccaaat cagggaacta acgtactcat tcaggaaaat tgccccggat aaacaaattc
     2281 caaacacgac tcctagccgc cgatctctgc tccggttcgg aaacactatc gtgaaatatt
     2341 cctgtaaagc cccgaccgtc actaaaaaaa ctactgcggc aaacaaccat ggtgggctcc
     2401 agccaacgag aagcaccagc aacggcaggg cgagcgctgc cgtcataact ctggagctaa
     2461 gattgccgtc caccggcacc tagtcgaaga gtagaatcgt cgatctgttc gtcagttcga
     2521 ccaaatctcc gccgccgacg ctggtagtcc agcaacgccg taagatattc cggctcccga
     2581 aaatcgggcc ataacgtcgg ggtcatgtaa agctcgctgt aggcgagctg ccagagataa
     2641 aaattgctga tgcgcatttc gccgctagta cggatcaata ggtccgggtc cggtactccg
     2701 caggtgtcca aatgtgccgc aaccatctgt tcatcgatta tgtgggcatc gatcttgccg
     2761 tcgcgcgctt gaagagccaa gtcttttgcc attcggacaa tttcgtcccg cccgctgtag
     2821 ctgagggcga ggatcaccgt catacgttga ttctcgcgcg tgagttctat gactttttct
     2881 agaatcgtaa gaacgttggc gggcaggcgt gatcgatcac caatcgccag cagacgaatg
     2941 ccgtagcgca tcatcttcgc ctgctctgcc gcaagataat gttccagtag ccccattaag
     3001 gcgtcgacct catcgtgagg acgaaaccaa ttctcggtgg aaaacgcgta aagcgacaga
     3061 taacgaatgc cgattttgcg gctcatctcg acaataacgc gaaccgacgt tttgccccgc
     3121 cgatgacctt cgatgcgatt cttgccgcgc agtttcgccc agcgtccatt gccgtccatg
     3181 ataatggcga cgtgtttcgg caggcgcgat ttatctaggt cctgtagctc cattgctgaa
     3241 tacgttcgct tcgcggcatc gggtgagaag ctctcagggt gagttcggtt ttaaacagga
     3301 aggacaaatc gatttggtag ctcgcaggcc cggaattaga ctgccatcac ttcttcttct
     3361 ttggatttaa caatcttgtc gattcggccg atgaaatcat cggtgatttt ttgtacgcgg
     3421 tcgtgagcgt gctttgaatc gtcggcggta atctccttct tcttttcggc atcttttacg
     3481 tgctcgatcg ctacacggcg gtgattgcgc actgacacgc ggtactcttc ggcaactttc
     3541 ttgacgtgct taacgagttc tttgcgccgc tcctcggtga gctgcgggat cggaatccga
     3601 atgattttac cgtcattgct gggcgtcaga ccgagatccg atttgaggat cgctttttca
     3661 atttcaccca tcgcgccgcg atcgaagggc tgaatcacca atagtcttgg ctcgggcgcg
     3721 ctcaaggttg cgagctggat cagtggcgtc gtcgcgccgt agtagtccac agtgatatgc
     3781 tccagcaacc cggtcgaagc gcgacccgtg cggatttttc ccagctcttt cctgagcgcg
     3841 cttaccgaac catccatttc cttttgcaat tgggtaaaaa tggcttcatt catcgctttc
     3901 ctccgcttac cagtgtgcca attggttcgc cggaaacgac tcgtttgata ttaccctttt
     3961 ccatcagatt aaagacaata atcggcaaat tgttgtccat gcagagggag atcgccgccg
     4021 aatccatgac tttgagctca cggttcagaa cttcgatata actcagctca tcgaattttc
     4081 gcgccccctc ggtcttgaac gggtcagcgt catagacacc gtcgaccttg gtcgccttga
     4141 gaatgacctc agcgcccact tccatcgccc gcaagctcgc tgtcgtgtcg gtggtaaaat
     4201 aaggattgcc ggtgccaccc gcgaagatca ccactctgcc cttttccaaa tgccgagtgg
     4261 cgcggcgcct gatgtaaggc tcggcgacct gtcgcatctc gatcgccgag agcactcgag
     4321 tcgagacgcc aagcttttca agcgcgtcct ggagggctaa gctattgatg accgtcgcga
     4381 gcatccccat ggtatcggcg ctcgcccgat ccatgccgat ctcactggca atcgcaccgc
     4441 gaaaaatgtt gccgccgccg atcaccaatg ccagctcgca cccgagctct ttcacttctt
     4501 tgatttcttg cgcaaaagcc ttgacggtgt ttccatcgat cccgaaattc agcggcccgg
     4561 caaatacctc cccggtaact ttgagaatga tccttttgta cttgggggcg ccgttgtcca
     4621 tgaacttatc ggtgccgcct actcctttgt cactgcctca cccaaatggt acctcgcgaa
     4681 ccgacgcacc tgaatgtttt cgccgagtcg cgcgatggcg tcgttgacga cgtcggcgac
     4741 ttttttatcg ggatctttga tgaacgcctg ctccaacaaa catacttcgg agtaaaatcg
     4801 atcaattttg ccgtcaacaa tcttatcgac tactttttcc ggttttccgg tttcgagagc
     4861 ctgttgccga taaatcgtct tttccttctc aagatgctca ggcgaaacct cctcgcgcct
     4921 gacataatga gggttggccg cagcgacctg catcgcaatg tccttgacca gcatctgaaa
     4981 ctctgcggta cgcgcgacaa aatcggtctc gcaattgatt tcgaccaaaa caccgatttt
     5041 gcctcccgga tggacataag cacctacagc gccatccgcc gcgacgcgat cggctttttt
     5101 cgcggcggca gctaacccct tctgacgcaa atagtcgatg gctttttgca cgtcgccact
     5161 gcactcgccc agggcttttt tacagtccat cataccggct ccggttttgt cccggagctc
     5221 tttgacggcg ctcgcgctga tttccacttt aaactcccgc cgcagccgaa aattcattca
     5281 cgttgggcgt atcggcgcgt tcttcttcct ttctcttggt caaagattgc tcgtacagcg
     5341 cccgcccttc attgatcgcg tcggcaaccg ccgcgcaaaa taatcggatt gcacggatgg
     5401 catcatcgtt ccccgggact ttgtagtcga ccatgtcggg atcgcaattg gtatcgatca
     5461 ccgagatcac gggaattccg agcttgcgag cctcctttac ggcgatctcc tcctgcttcg
     5521 ggtcgataac gaaaatggca tcgggaagct tgcgcatcga tttaataccg ccaagggcct
     5581 gttcgagttt ggctttctcc cgcttcagtc ccaacatctc tttcttggtc aatgcctcga
     5641 cgattttcgg gtcgttttcc atctcctcga gtttgcgcaa acgctcaatg ctttggcgaa
     5701 tggtcgaaaa atttgtcagg gtaccgccga gccaacgatt atggacatga tacatgccac
     5761 atcgctcagc ttcctcgcga atggcatcct gggcctgctt tttggtaccg acgaaaagga
     5821 cgtggcctcc cgaagccgcc aagtcgcgca ccaccgcttc caattctttg aacatcgtaa
     5881 cggtttgctg cagatcgata atatgaatcc cgtttcgtgc tccgaagatg tagggcttca
     5941 tcttcggatt ccagcggcta gtctggtgtc caaagtgcac gcccgcttcg agtagttgtt
     6001 tcatggaaat atcactcatg cttgtctcct tggtttttcc tccctttcgt catttcctca
     6061 tcggacccga aggcacctcg atggggatca gtaaaggtgt gaatttgtga accggacagt
     6121 tcccgtcggc tggctactgc cgaaccgccg cgaaccgaaa attgtcaaag ataacgttgc
     6181 gttattaaca gagtttagcg ggtgacgcaa gaatgaacag atgtgggtgg ctattgattc
     6241 atagattcga gaaattcctt gttggttttg gtgccgcgca tcttgtcgag cagaaactcc
     6301 atcgcctcga
//
LOCUS       DASULZ010000368         6073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_858038, whole genome shotgun sequence.
ACCESSION   DASULZ010000368 DASULZ010000000
VERSION     DASULZ010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858038"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            316..1419
                     /locus_tag="VFY96_07630"
     CDS             316..1419
                     /locus_tag="VFY96_07630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5606368.1"
                     /translation="MSILNVAGVSKFYKDVRALDDVTFEFEKGIISFLGPSGCGKTTL
                     LRSIAGLEIPDSGSIAIADKIQTSIADGILVPPYSREIGFVFQNYALWPHMTVFNNVA
                     FGLKLRKKSADEIKRKVMAALELVGLQGREQRFPSQLSGGQQQRVALARSLALEPRLI
                     LLDEPLSNLDAKLREEMRVELKKLIKQVSISALYVTHDQEEAFTISDAVVVMEAGKIL
                     QYGAPDEIYNRPAHPFVASFVGHSALVDGSVVRVEGENCLVNIPEFDGVNLVSPAPRS
                     AVVGNACKVVIRTTEVKLSHDGFPDGADNVLEGMMTSREYRGGLTDHRIQIGSKELVV
                     TSHKFCPMIRVNGDGGKTFVSIDRSAISVILAQ"
     gene            1516..3168
                     /locus_tag="VFY96_07635"
     CDS             1516..3168
                     /locus_tag="VFY96_07635"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX5606369.1"
                     /translation="MPLVGSAIVGAAIITPVLVVFWRSFTTGRLGFNVGLNIANYLRV
                     FGDKDIMAMLGNSVVYAGGSALLGTGVGALLAWIVARTNTPGKTLVELLPLYPILMPP
                     IMKNIAWILLLAPRSGILNGMMQQFFGIETLIFNAFSMTGMIWVFGLACVPLGYLFLL
                     PVFLSFDPSLEESAYIAGSKPVHTMLKITFPLALPAFLSAFVLNFLRGLRSFETPVLQ
                     GTPANIKVFVSRVYDSMALEFNTGLATAYSVVLVALSVITLYFYVRATRFSDRYATIT
                     GKGYRVKVIDIGPWKYLTFFLVFIYFLVGIAIPFIVLIVVSMIPYFDYDTFMKFPTNA
                     VLTNYYTVMRHPSFVTGLYNSLVLSVTIAIVTVLAGIVMAFTIYRTRASGTRIFEFIG
                     TLPLAFPPLVLSVGLVIIFLGTPLYNSLWALGIGLFVAYFPYAFRNASGSIVNIHKEL
                     DEAAWVHGARWRHVFFKITLPILKPSVGGALFYIFIEAIRNVDVAVLLTSPGKEYGPV
                     TLFEYFRVGQWAEAAAGGVIYLIILIVAVSIAKFSFKMKFSL"
     gene            3227..4375
                     /locus_tag="VFY96_07640"
     CDS             3227..4375
                     /locus_tag="VFY96_07640"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5606370.1"
                     /translation="MIVAVCATLLIAAAAPAAEKAATKEQSKDAATKDQKPAAKAPSR
                     EQVRKESGSASATAAAAKAFEKVSAELYPKAKQEGNLIVYSVWDVEHLKVITDAFMKR
                     YPGIKATYWQGRNPEIVTRALTEFQGGQASWDVVLSDNAPPVIRAAGAIMNYETVQKD
                     VLYLHDPTMPTVSLQVQALAYNTKKMKPADLPKSWEDVANPKYKGIVALDDPLRAGPL
                     SSMLSGLKTQWKDDNRYSNFVKGLKALNVPVHQSTSAMFRLVISGEYSICMPALLHDV
                     FEEIHKGTPVNYNKAVPPVVFPRQAGIYVKAPDPNAAKLFAEWLISEEGQKAIDSVGR
                     ETSRNDFKSHTSIEAAFGKNIKPIPVTDQNFLEDPKKWLDTNVKPIWG"
     gene            4526..5164
                     /locus_tag="VFY96_07645"
     CDS             4526..5164
                     /locus_tag="VFY96_07645"
                     /inference="COORDINATES: protein motif:HMM:NF020905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HEX5606371.1"
                     /translation="MALLKTDVIAKNSGTAFEIKKGQRLRIAGRTIVDFVAFNLHDLS
                     ERFDQARTKTNQVKIFISTGDVLYSKRNNPMLTIIDDTFTEGRHDLQKGMCSRKRFEM
                     VARGESKRIFAEGVDINPKKADQIPDHGCWENLSDAVKPWNIAPDDVPSPFNIFQCMR
                     IDPETGVMYDTMIRPKVEAHVDFRAEMDLLVAASACPESGRGQAIRVEVYDR"
     gene            complement(5245..>6073)
                     /locus_tag="VFY96_07650"
     CDS             complement(5245..>6073)
                     /locus_tag="VFY96_07650"
                     /inference="COORDINATES: protein motif:HMM:NF024430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3891 family protein"
                     /protein_id="HEX5606372.1"
                     /translation="QGMVIRELKDGRCYVALQEDHAELAAQFAAHWGNDRFSKLRPYQ
                     TMVFATTYHDSGYREWEGTPPMNVQKGRPYAHREDVPNFESVELNAYAKNVDWLSSQD
                     RYAGLLVSMHRTGLWHNRYNIFTEPAGRLRDRSQAVQNAKKELEAKQQEVKKSLAAKH
                     PEFENELAYNYMALQIFDLLSLYFCCDGYASNEEFKEYAIAPVRVSYDRDDTVRLTIR
                     PNGSGSVVIDPYPFDLSPLKFSARARIVAPPKEKSESACVDAYHRAPRQMLTFEVSA"
BASE COUNT         1380 a         1559 c         1710 g         1424 t
ORIGIN      
        1 gattcgcgaa cggctcagct ggcaagtaga agtcgcgaag taccagcaga aggtcccgct
       61 gcaatagtgt gcgtgaggaa ccatctgtgc ctggatgatt tccgacacta aattgcccgc
      121 agcaactgcc aaagaattag tagcataggt ggtggacttg gcctgcgtgt gcgcgcaact
      181 ctaacttcgc ccgacctcgc ccacggttcg gattgtttca tttctcaagt aattagagta
      241 agtaaagtaa cagaattatg cgatttcgac ggagctggcg tagcttttgc gctgccggat
      301 tacgggtgga aaacgatgtc gatccttaat gtcgctggcg tttcgaagtt ttacaaagac
      361 gtaagagcgc tcgacgacgt taccttcgag ttcgaaaagg ggattatctc gtttttaggg
      421 ccctcgggtt gtggcaagac gacgctgctc agatcgattg ccggactgga aattcccgat
      481 agcggatcaa ttgcgattgc cgataaaata cagacctcca tcgcggacgg aattttagta
      541 ccgccttatt cacgcgaaat tggcttcgtc tttcagaact acgcgctctg gccgcacatg
      601 acggtcttca acaatgtggc cttcggtctg aagctgcgca agaaatccgc ggacgaaatc
      661 aagcgcaaag tgatggcagc gctggagctt gtcggcttac aagggcgtga gcagcgcttt
      721 ccctcgcagt tgagcggtgg acagcagcag cgggtggcgc tggcgcgcag tctcgccctc
      781 gagccgagat tgattctgct ggatgagccg ctgagcaacc ttgacgctaa attgcgcgag
      841 gaaatgcgag tggagttgaa gaagctgatc aagcaagtga gcatcagcgc gctgtacgtc
      901 acccatgatc aggaagaagc gtttactatc tccgacgcgg tggtggtgat ggaagcggga
      961 aaaattcttc agtacggcgc gcctgatgag atttataacc ggccagcgca tccattcgtt
     1021 gcgtcgtttg tggggcattc ggcactggtc gacggcagcg tcgtccgggt cgaaggagag
     1081 aattgtctcg ttaatattcc ggagttcgac ggcgtcaatc tcgtctcacc cgctccccgc
     1141 agtgcagttg taggcaacgc ttgcaaagtc gtcatccgca cgaccgaagt caagctcagc
     1201 cacgacggat tccccgacgg cgccgataac gtgctcgaag gcatgatgac cagccgcgag
     1261 tatcgcggcg gattgacgga tcaccggatc cagatcggtt cgaaggagct agtcgtcact
     1321 tcccacaagt tttgcccgat gatccgcgtg aacggcgatg gcggaaaaac ttttgtgtcg
     1381 atcgaccgct cggcaatcag cgtcatcctc gcccaatgaa acccgcaagc ctataaaaat
     1441 ccttctcaag ccgtcgagga gattcgtacg tccaggggaa attccgtcaa aaatgccttt
     1501 cgacagggca cgctcgtgcc gctcgtggga agcgcgattg tcggcgcagc catcatcaca
     1561 ccggttttgg tcgtgttttg gcggagcttc accactgggc gtttgggatt taatgtcggc
     1621 ctcaatatcg ccaattatct gcgcgtcttc ggagacaaag acattatggc gatgctcggc
     1681 aattcggtgg tttatgcggg cggatcggcg ctgctcggca caggtgtggg cgctctgttg
     1741 gcgtggatcg ttgcgcgcac gaacaccccg gggaaaacgc ttgtcgagct attgccgctt
     1801 tatccgatat taatgccgcc gatcatgaag aacatcgcgt ggattttact gctggcgccg
     1861 cgctcgggaa ttctgaacgg gatgatgcag cagttcttcg gtattgagac gctgatcttc
     1921 aatgcgttca gcatgaccgg gatgatttgg gtgtttggcc ttgcgtgtgt gccgttggga
     1981 tacttattcc tgctgccggt ttttctatct ttcgatccct cactggaaga aagcgcctac
     2041 atcgcgggca gtaagccggt gcatacgatg ctgaagatca cgtttccgct ggccttaccc
     2101 gcgtttctct ccgcgttcgt gctgaatttt ttgcgcggcc tgcgttcgtt tgaaactccg
     2161 gttttgcaag gaactcctgc caatatcaaa gtgtttgtca gtcgcgtcta cgattccatg
     2221 gcgctggagt tcaacacggg tctggcgacg gcttacagcg tggtcttggt ggcgctctcg
     2281 gtgatcacgc tctattttta tgtgcgcgcg acccggtttt ccgaccgcta cgcgacgatt
     2341 accggaaagg gttatcgcgt caaggttatc gacatcgggc cctggaaata cctgacattc
     2401 ttcctggtat ttatctactt cttggtcggc atcgcgatac cgttcattgt gctgatcgtc
     2461 gtgtcgatga ttccgtactt cgattatgac accttcatga agttccccac caacgcggtg
     2521 ctgaccaatt actacacggt tatgcgccat ccaagcttcg tgacggggct ctacaactcg
     2581 cttgttttgt cggtcaccat tgccatcgtt actgtcctgg ccggaatcgt tatggccttc
     2641 accatctacc gaacgcgagc gtccgggacg agaatctttg agttcatcgg gacgttgccg
     2701 ctggcttttc cgccgctggt tttgtccgtc ggcctggtga tcatcttttt gggcactccg
     2761 ctgtacaaca gcctgtgggc tttagggata ggtcttttcg tcgcttactt tccatacgct
     2821 tttcgcaatg cttccggctc tattgtcaat attcataaag aattggatga agcggcgtgg
     2881 gtgcacggcg cgcgctggcg ccacgtgttt ttcaaaatca cgctgccaat tttaaagcca
     2941 tctgttggag gtgcgctttt ttacattttc atcgaggcta ttcgtaatgt cgatgtcgcg
     3001 gtattgctga catcgcccgg aaaagagtat ggtccggtaa ccctgttcga gtatttccgg
     3061 gtcggacaat gggccgaagc ggcggccggt ggcgttattt atttaattat tctgatcgtt
     3121 gcggtgtcga tcgctaaatt ttcgttcaaa atgaaattca gtttgtaagt ctcgcgctct
     3181 atagaagtaa agaaagggga aaacaagcat ggtcaggaca gcaaaaatga tagtcgcggt
     3241 gtgcgcgacg cttttaattg ccgcagcggc accggcggcg gaaaaggcgg caacgaagga
     3301 gcaaagcaaa gatgccgcga cgaaggatca gaaacccgct gctaaagctc cttcgagaga
     3361 acaagtacgg aaagaatcag gcagcgcgtc tgcgactgca gcggcagcca aggcgttcga
     3421 gaaggtgagc gccgagcttt atcccaaggc gaaacaagaa ggcaatttga ttgtttattc
     3481 ggtctgggat gtcgagcatc tcaaagtgat caccgatgct ttcatgaaac gctatcccgg
     3541 catcaaagcg acctactggc aggggaggaa tccagaaatt gtcacccgcg cgcttacgga
     3601 atttcagggc ggacaggcca gctgggatgt ggttctgtcg gataatgcgc cgccggtgat
     3661 acgggcggca ggggcgatca tgaactacga gaccgtccag aaagatgttc tctatctgca
     3721 cgatccgacg atgccaacgg tgagcctcca ggtccaggca ctcgcttaca atacaaaaaa
     3781 gatgaagccc gcggatctac cgaaaagctg ggaggacgtc gccaatccca aatacaaagg
     3841 gatcgttgct ttggacgatc cgctgagggc agggcctctg agctcgatgc tctccggact
     3901 caaaacccaa tggaaagacg acaatcgcta cagtaatttc gtcaagggtt tgaaggccct
     3961 caatgttccg gtgcaccaga gcaccagcgc gatgtttcgc ttggtgatct ccggtgaata
     4021 ttcgatctgt atgccggctt tgctgcacga tgtgttcgag gaaattcaca agggaacacc
     4081 ggtgaattac aacaaggccg tgcctccggt ggtctttcca cgtcaagccg gcatttacgt
     4141 caaagcgccg gatccgaatg cggctaaatt gtttgccgaa tggttgattt cagaagaggg
     4201 ccagaaagcg attgattcgg tcggacggga aacatcgcgc aatgatttca agtcgcatac
     4261 ctcgattgaa gccgcgttcg gcaaaaatat taagccgatc ccggtcaccg atcagaattt
     4321 tctcgaggat ccgaaaaaat ggctggatac caacgtgaag ccgatttggg gataacgtaa
     4381 agcgcaactc gcgcacaaag ccatgcgggc agcatcgctc gcatggcttt tcaatttgtg
     4441 ccgttgacat gacttttcct gcaggtcgac gctgccgaga gaagtgaacc cgtgcttagg
     4501 acgtccgcgc aaggaggaga ttcaagtggc tctcttgaag accgacgtaa tcgctaagaa
     4561 ttcgggcacg gcgttcgaaa taaaaaaagg ccagcgcttg cggattgctg gaagaaccat
     4621 cgtcgatttc gtggcgttca acctgcacga tctctcggaa cggttcgatc aagcgcgaac
     4681 caaaaccaat caggtcaaga tcttcatcag caccggcgac gtgctctatt caaagagaaa
     4741 caacccgatg ctcaccatca tcgatgatac cttcaccgaa ggacgccacg atctacaaaa
     4801 aggcatgtgc agccgcaaac gtttcgaaat ggtcgcgcgc ggagaatcaa agcgtatctt
     4861 cgcggaaggc gtcgacatca atcccaaaaa ggcggatcaa attcccgatc atggctgctg
     4921 ggaaaatttg agcgacgcgg taaagccgtg gaacatcgct ccggatgacg tgccgagccc
     4981 gttcaacata tttcagtgca tgcgcatcga tccggagacc ggcgtgatgt acgacacgat
     5041 gatccggccg aaggtcgaag cgcacgtgga ttttcgcgcg gaaatggatt tgctggtcgc
     5101 ggcgagcgcc tgtcccgagt cgggcagggg acaggcgatt cgtgtggaag tttacgatcg
     5161 ataaagggtt tcaccgccga ggcgcagaga acgccgacga agaaactctg tttcctctgt
     5221 gtctcagcgg taaatagttc ttcgtcacgc ggaaacttcg aaagtcagca tctggcgggg
     5281 cgctcgatga tacgcgtcca cgcaggcgga ttcgctcttt tccttcggcg gcgcgacgat
     5341 gcgagcgcgc gcggaaaatt tgagcgggct caaatcgaag ggatacggat cgatcacgac
     5401 cgagccgctg ccgtttggtc ggatcgtcaa ccgaacggtg tcatcgcggt cgtatgaaac
     5461 gcgcaccggc gcgatggcat attctttaaa ctcttcattc gaagcgtagc catcacagca
     5521 gaaatagagc gacagcagat caaagatctg cagcgccata taattatagg cgagctcgtt
     5581 ttcaaattcg ggatgcttgg cagccagtga tttcttcact tcctgctgct ttgcttccaa
     5641 ttctttcttg gcattctgca cggcctggct tcggtcacgt aatctcccgg cgggttccgt
     5701 aaaaatattg taacggttgt gccaaagtcc ggtgcgatgc atcgaaacca gcagcccggc
     5761 atagcggtcc tgagaactta gccagtcaac gtttttggcg taggcgttga gctcgacgga
     5821 ttcaaagttg ggcacatcct cgcgatgggc atagggacga cctttttgca cgttcatggg
     5881 cggcgtccct tcccattcgc gataaccgct gtcgtgataa gtggtcgcga acaccatggt
     5941 ttgatagggg cgcagcttgg agaaacgatc gttgccccag tgcgcggcaa actgcgccgc
     6001 gagctccgcg tgatcttcct gcaaggccac gtagcaacgg ccatctttca gttcacgaat
     6061 taccatacct tgc
//
LOCUS       DASULZ010000369         3068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1059874, whole genome shotgun sequence.
ACCESSION   DASULZ010000369 DASULZ010000000
VERSION     DASULZ010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059874"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2123
                     /locus_tag="VFY96_07655"
     CDS             <1..2123
                     /locus_tag="VFY96_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606373.1"
                     /translation="NELPLHRADLSGYGLSTFSDWHQNVPTGITKVRFDVLNGRTSYE
                     VIQAKTILAFPLCYAVRTIIFERRNSGKVLRFDSGWKPVNDGEFRVEFQPNLADPERN
                     FEFEKGVVRAFRNIRRIRVLANPSIPLSDSSVWQPVIFDADADLDGVIAGAGGVVPIY
                     DHPGYIQLNPTGAGSEVTRTRIVRLLKAIGKAVGGGIDCRIRAGGTLEMHLSGIFADE
                     ASDDNPFKPIALMLAAYGLPKLPRAGQWSAVRINGSTSEASPVDPRHGIPLVRRTGQK
                     KYIFVDPGEVNRSTRDEFGFLMSTATSRVLFPRPSVDPDEAGTLRTAAPNVADPYSLV
                     QSTSQFPRPNYALRCAELPVFSVSSADEWKLTNPNFKFIKPAQELAKGGEWDMSRGFD
                     PAQGIDLLLDSAIQNKPWEIAATPNDLDINIDGIGKIFTIKTAYTALSGAVPKLDKPA
                     LDFGPALDAAKDVVSALSNFTGMDNFHVDVDVNAGSGPSPSFLVTLKLKFRIGEGPNE
                     RIDIGVGKFYGEFIIRGELEAALSGKARGLLSAEFQGDVQQGIIPPAIYAGGFFRFAI
                     QIDETGKPTIELGLGTTASIGGDLIKGLLEVEVTVRYGYTLIPQTLEPGVFLGLEARA
                     KLLGGLVGFSFSVEAMARIKRVSIEANELVVWAQIRVCASVTVAWLVDEDIDVKTQFE
                     QTLPLAFVAAAAFAPALLPAAALL"
     gene            2400..>3068
                     /locus_tag="VFY96_07660"
     CDS             2400..>3068
                     /locus_tag="VFY96_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606374.1"
                     /translation="MPKVAPLDPLELGQPSFAQANLVFEANVIRGLDRLPRAVDATGL
                     GELGLISDPPINKEALFNELDQEFNIKKPVRGPAVSQRFLKPVTSSYLALTGGRQLSE
                     DLAGEKDFECALHDAQASQPPEPEVLKDEVTWGQLMAYALRQPKLATALGLVGEAEIN
                     LEDPAVFEKGGWLYLSLHASSDYAAVVEPPPFKALYAARIPPLNQKRSLYAAVLFPVD
                     GGGVA"
BASE COUNT          680 a          850 c          841 g          697 t
ORIGIN      
        1 tgaacgagct tccgctccac cgcgcggatc tatcaggtta cggcctcagc acgtttagcg
       61 attggcatca aaatgttcca accggaatca cgaaggtacg tttcgatgta ctcaacgggc
      121 gcacgtcgta cgaagtaatc caagccaaga caattcttgc gtttccactc tgctatgccg
      181 ttcggacaat catcttcgag aggcgaaact ccgggaaggt tctacgtttc gatagcgggt
      241 ggaaaccggt gaacgatggc gagttcagag ttgaattcca gcctaacctt gccgatccgg
      301 aaagaaactt cgaatttgaa aaaggcgtgg tcagggcttt tcgtaacatc cgccgcataa
      361 gggtccttgc gaatccgtca ataccattgt cggattcgtc cgtttggcag ccagtgatct
      421 tcgacgccga tgcggatctc gatggtgtca tcgctggagc aggcggcgtt gtaccaatct
      481 acgatcatcc cggctacatt caattgaacc ctaccggagc cggttctgaa gtaacacgca
      541 cacgaatcgt gcgactgctc aaagcaatcg gaaaagcagt gggcggaggc atcgactgca
      601 ggattcgagc gggcggcacg ctcgagatgc atctctccgg catcttcgcc gacgaagctt
      661 cggacgataa tccattcaaa ccaatagcac tcatgctcgc cgcatatgga ttgccgaaac
      721 tgccgcgggc cgggcagtgg agcgcggtgc gaattaacgg ctcaacttct gaagcttcgc
      781 cggttgatcc tcgacacggc attcctctag ttcgtcgcac cggtcagaag aagtacattt
      841 tcgtcgatcc tggtgaggtt aaccgctcca cgagggacga attcggtttc ctgatgtcga
      901 ccgcaaccag ccgcgtcctg tttcccaggc cgtcggttga tccggatgaa gcgggcacgc
      961 ttcgcaccgc ggcgcccaat gtggctgatc cctattcgct ggttcaatcc accagccagt
     1021 ttccgcggcc aaactatgcg ttgcgttgtg ccgaattgcc ggtattcagt gtttccagcg
     1081 ccgacgaatg gaaactaacc aatcccaact tcaaattcat aaagccggcg caggaactag
     1141 ctaaaggcgg cgagtgggac atgtcgagag gctttgatcc tgcgcaaggt attgaccttt
     1201 tgctcgattc tgcaattcag aacaagccct gggagatcgc agcgactccg aacgatctcg
     1261 acatcaacat cgatggtatc ggcaagatat tcaccatcaa gacagcctac actgccctca
     1321 gcggcgccgt gccaaaactc gacaagccgg cgctcgactt tggtccagcg cttgacgccg
     1381 caaaagacgt cgtcagcgcc ttgagcaact tcaccggcat ggacaacttc catgttgatg
     1441 tagatgttaa cgccggctcc ggcccgagcc cgtccttcct tgtcaccctt aagctgaagt
     1501 ttcgcatcgg tgaaggacca aacgagcgca tcgatatcgg cgttggtaag ttttatggcg
     1561 agttcataat ccgcggtgag ctggaagcag cattgagcgg gaaagcgcgt ggtctgctct
     1621 cagctgaatt tcagggtgat gtgcaacagg ggatcattcc acccgcgatt tatgcgggcg
     1681 gcttctttcg tttcgccata cagattgatg aaaccggcaa accgactatc gaacttggat
     1741 tgggaacaac cgcgtcaatc ggcggcgacc tgatcaaagg cctgctcgaa gtggaggtga
     1801 ccgtcaggta cggatacacg ctaattccgc agaccctcga gcccggcgtt ttcctgggac
     1861 tcgaagcgag agcaaaacta ctcggcggcc tggttggttt ttcgttctcg gtagaagcga
     1921 tggcaaggat caaacgcgtc tccattgaag ctaatgagct tgtggtttgg gcacagatcc
     1981 gcgtttgcgc ttcagtcaca gtcgcgtggc tcgtcgacga ggatatcgac gtcaaaaccc
     2041 agtttgaaca gacgcttccg cttgcgttcg ttgcggctgc cgcctttgcc ccggcgctgc
     2101 ttccggccgc cgctttgtta taaggttata agggctttgc acaaaaagtc cttgttcctg
     2161 gatttctctg tgcaacctct gtgtcctctg tgtctctgtg gtgcggtttt actcggaatt
     2221 catcaaccac agagacaccg agaacacaga ggttgcacag agaagagcgc gatcgagact
     2281 ttttctgcaa agccgttata aggaaatctt ttatgccatt aggaggccca tctaaattag
     2341 gcatactcgc gttcccgcag agttgggagc caaacaccct gcgtgtccgc ttcctctgta
     2401 tgcctaaggt cgctccgctc gatccactcg aacttggtca gccgagtttt gcccaggcga
     2461 acctggtttt tgaagcaaat gtgattcgcg gtctcgaccg tctgcctcga gcggttgacg
     2521 ccaccggact tggtgagctc gggttgatta gtgatccgcc tattaataag gaggcgctgt
     2581 tcaatgagct cgatcaggag ttcaacataa aaaagccagt gcgcgggccc gccgtttcgc
     2641 agagattctt aaaaccagtt accagcagtt atctcgcgct caccggtggc cggcaactgt
     2701 ctgaagatct ggcgggggag aaagactttg aatgcgcgct ccatgatgcg caagccagcc
     2761 aacctccaga gcctgaggtc ttaaaggacg aggtcacgtg gggccaactc atggcttatg
     2821 cgctccggca gccaaagctg gcgacggccc tcggactggt cggagaagct gagatcaatc
     2881 ttgaagatcc ggcggtcttc gaaaaaggcg gctggctcta tctgagcctg cacgcctcga
     2941 gcgattacgc ggcggtcgtt gagccgcctc ccttcaaggc tctttacgcg gcccggattc
     3001 caccactcaa tcagaagcga tcactttacg ccgctgtgct ctttccggtt gacggcggag
     3061 gtgtagcg
//
LOCUS       DASULZ010000370         5174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1059939, whole genome shotgun sequence.
ACCESSION   DASULZ010000370 DASULZ010000000
VERSION     DASULZ010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5174
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059939"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            365..1198
                     /locus_tag="VFY96_07665"
     CDS             365..1198
                     /locus_tag="VFY96_07665"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5606375.1"
                     /translation="MQLRPIQWPNEAKIACSFFVALEAFEKSSQYRRSEDGKPDYASL
                     AYGEYGGKAGIWRIMDVFSRHGVKGTIDTNGRIAEDFPDVVKELHSAGHEIVGHGWVN
                     DTAQSSLDADSEKKLIRRTLDALASVTGKRPVGWVSPGHRINEYTLGHYVDEGIYWTG
                     DLPGPDIPQHRMVNGKPIVIMPRLDYANDLSLLFTHKNPAQFYFDSFKAAFDWLYAEG
                     QAGAPKLIDCLVHAHIGGRPNIIGVFEQCIRYAKGFKDVWFCTKGEIAKYYLDRYVTK
                     A"
     gene            1398..2570
                     /locus_tag="VFY96_07670"
     CDS             1398..2570
                     /locus_tag="VFY96_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5606376.1"
                     /translation="MNRATEKFLASLKSNELLLKLTWDEWLAIDPPYIPTGLTTADAW
                     TRYQRNGYDWDMHGTVYTPEKETDARRAFVFFHGGAGSEKIMDLTPDGRPGLARVVAS
                     QGFKVLALTYPGHYPLSGVWQQSIPERKPTYLKDRDLPNDEISDRNLKCTFNVILHGA
                     GLLTDEHLAGREIIVFGHSTGGPMAAHLTRFSKNTRVVGIVGFGSGGPDGWRLEWRNK
                     TGAEKYAEKPVDHISRRSPDSFKESGYIDPEDLTPWGGADDYIRLVSPLRSQMKTSLC
                     DNQHNAAVGILEEYPKRTGLPREEYFDHLQEPDQNWLKTIRVLLTVGENDKGHWVRGE
                     TLEDKREMFMGAKYAAVTRGAHVVLIPRYGHVGYAELHNEKIAYLWLWAYKQGYFD"
     gene            2596..3666
                     /locus_tag="VFY96_07675"
     CDS             2596..3666
                     /locus_tag="VFY96_07675"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5606377.1"
                     /translation="MTFRQSAVLVALLIVFQMSANLAAQTKSMSSAELALYKGKDREA
                     ILVDGAKKEGQVTFYTSNTWMAGPVSQEFGKKYPFIKTNVWRSDSKELLKRLTDEAAA
                     GRFIADVVETSPDYMALMIREKLFQEVFSPELAPYDNNSKVKGKNGFFHWTSRELYIS
                     LGFNTNLAPPAEAPKTMKDLLEPKWKGKMTIAGTTTGTQWIGAVVEAYGREFLEKIAK
                     QDVNVQNISGAALAELVASGEVPLSPTIFNSNISVAKQKGAPVEWRPIDPVVATTGTS
                     GMVVKAPNPHAALLFLDYLHSKEGQEVVLKGGLSSARKDVASADQKFKKVYIENKYAP
                     DVMERKFAEWEGLKRRLFISKR"
     gene            3684..4628
                     /locus_tag="VFY96_07680"
     CDS             3684..4628
                     /locus_tag="VFY96_07680"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5606378.1"
                     /translation="MQHKVLITGANGFIGSHTSRLLESLGHSIIPIDVVARSPDLSLL
                     GIKTPSHLLNVTDQREFRALCEREEPTHIFHAAHPPRDESPSVIDYCYHAMTNILEAA
                     KDLKIKRVVYSSSASIYGQLQKPAGSLVKEDDAVSIYPTYFYRAAKTVSEWMGDFYKE
                     KYAVDFVALRYSSVYGPGLYRSIPLELKRGILGQHCRPFLTRPNDDLIYIDDVVDAVS
                     RALFTDKRLSWAYNIGLDKTYVSEDLKRAIEKALPHLQFEIGEHPNAAEVAPHRLRSP
                     LDISLARRELDWKPKIYLEEGIARLTAWLLEHKNKLQQ"
     gene            4651..>5174
                     /locus_tag="VFY96_07685"
     CDS             4651..>5174
                     /locus_tag="VFY96_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606379.1"
                     /translation="MNRRQRAWMLICCAVQTILLATFACAATAPMPPAQLALYQGADR
                     EKIFIEGAKREGQFTLYTSHTWFKTFVGEFEKKYPFVRVSQWRNDSKNIIRKVLEEAK
                     AGRVLADIVETTADGMGLLRREGLFQEYYSPEARYYPNELKAKGKNGFFYLPNRETYN
                     SLGFNTNLIPPETAP"
BASE COUNT         1234 a         1429 c         1373 g         1138 t
ORIGIN      
        1 tagcatccgt tccattctct gacacaaaat gtggagaaag attcccggta ccgtcagatt
       61 gtgtaaaatt cattgtcgcg ccagtttgcc atctcgtaaa agctgtattg ctcttcgcaa
      121 cgcgagttcc gaccactggt atccgataat tcttcaaata ggttgacctc cttcactgct
      181 gcttgatagt aattggcatc gttccgagtt cttagttgat ggttccgacc agcagaatct
      241 tgtcttcgcc gcagcacctt tggccgttcg ggctgcaaca gtatgcgcca gccacgaacc
      301 cattgttcac gagctatttc ggaagatctt cggcgcgccg aaattctaaa aagaggttga
      361 ccgtatgcaa ctgaggccga ttcaatggcc caatgaagcg aagatcgcgt gcagtttttt
      421 tgtcgcgttg gaggccttcg aaaaatcctc ccagtatcgc cgcagtgagg acggcaaacc
      481 cgattatgca tctctggctt acggtgaata cggtggcaaa gcggggatct ggcgtatcat
      541 ggatgttttt tcacgtcatg gcgtcaaagg caccatcgat acgaatgggc gcatcgccga
      601 ggactttccc gatgtggtga aggagcttca ttcggccggt cacgagatcg tcggacatgg
      661 ttgggtcaac gacaccgcgc aatcgtcatt ggatgccgac tcggagaaga aactgattcg
      721 aagaactctg gacgccctcg cctccgtaac cggcaagcgc cccgtcggct gggttagccc
      781 cggtcatcga atcaatgaat acactctcgg ccattacgtg gatgaaggca tttattggac
      841 cggtgatttg cccggccccg atatcccgca gcatcggatg gtcaacggca aacccatcgt
      901 gatcatgccg cgattggatt acgccaacga tttgagtctc ctgttcacgc ataaaaatcc
      961 cgcgcaattt tattttgaca gcttcaaggc cgcctttgac tggctctacg ccgaagggca
     1021 agccggcgcg cctaaattaa tcgattgtct cgtccatgcc catatcggtg ggcggccgaa
     1081 catcatcggc gtgtttgaac aatgcatccg ctacgccaag ggttttaaag acgtctggtt
     1141 ctgcaccaaa ggcgagattg ccaaatatta tcttgatcgc tacgtcacca aagcgtagca
     1201 cgatgtcaaa ataactcgga atggttcgac acttcgactt cgctcagtgc aggctccctc
     1261 gaccagcctt agtcccgagc ttgtcgaggg ctcagcatgc acggatttct ggctaatatc
     1321 ttaagttttc tccgctcctc tttagcttgt cgaaggactt caaacgagca ttcaacagct
     1381 tcctagaggt tcgatcgatg aatcgcgcaa ccgaaaaatt tctcgccagc ctgaaatcaa
     1441 acgagctgct gttgaagctc acctgggatg agtggctcgc tatcgatcct ccgtacattc
     1501 ccaccggtct gaccacggcc gacgcctgga cccgttatca acgcaacggc tacgactggg
     1561 acatgcacgg caccgtttac acgccggaga aggaaacgga cgcacgccga gcgtttgttt
     1621 tctttcacgg cggcgctggc agcgaaaaaa tcatggatct gacgcccgat ggcaggcctg
     1681 gcctggcgcg tgtcgtggcc tcccagggat tcaaggtcct ggcgctcacc tatccgggcc
     1741 attacccgct gagtggcgtc tggcagcaat cgattcccga gcgcaaaccg acttacctca
     1801 aggatcgcga tctgccaaac gacgaaatct cggaccgcaa tttaaaatgc accttcaacg
     1861 tcatcctgca cggcgcgggc ttgctcaccg atgagcattt ggccgggcgg gagatcatcg
     1921 ttttcggtca ttccaccggc ggcccgatgg ctgctcatct cacgcgcttt tcaaagaaca
     1981 ccagagtcgt cggcatcgtt ggcttcggca gcggtggacc ggatggctgg cgccttgagt
     2041 ggcgcaacaa aaccggcgct gaaaaatacg cggaaaagcc cgtcgatcat atatcgcggc
     2101 gctcgcccga ttctttcaag gaatcgggtt atattgatcc ggaagatttg acgccatggg
     2161 gcggcgccga cgactacatt cgtctggtga gcccgctgcg ctcgcagatg aagaccagcc
     2221 tctgcgacaa tcaacacaat gccgcggtgg gcatactcga agagtatccc aagcgcaccg
     2281 gcttgccacg cgaagaatat ttcgatcatt tgcaagagcc cgatcagaac tggctgaaga
     2341 cgattcgggt gctgctcacg gtgggcgaga acgacaaagg ccattgggta agaggcgaga
     2401 cgctcgaaga taagcgcgaa atgtttatgg gcgcaaagta tgccgccgta acgcgcggcg
     2461 cgcacgtcgt gcttattccg cgctatggcc atgtgggcta cgccgaattg cacaatgaga
     2521 aaattgctta cctctggctc tgggcataca agcaaggcta tttcgattga tgcgaccgct
     2581 caggaggatt cttcgatgac ttttcgacaa tcggcagttc tggttgcgct gctaatcgtt
     2641 ttccaaatgt ctgcgaatct ggcggcgcaa acaaaatcca tgtcctcggc agagttggcg
     2701 ctctacaagg gaaaagaccg cgaagccatt ctcgtcgacg gggccaaaaa agaggggcaa
     2761 gttacgtttt atacttcgaa tacctggatg gccggtccgg tttcccagga gttcggcaaa
     2821 aaatacccct tcatcaaaac caatgtttgg cgcagcgatt caaaagagct gctgaagcgt
     2881 ttaacggatg aggcagcagc gggccgcttc atcgccgacg tcgtcgaaac cagccccgat
     2941 tacatggcgc tgatgatccg tgaaaagctg tttcaggaag ttttttcccc ggagctcgcg
     3001 ccttacgaca acaactctaa ggttaaagga aaaaatggtt tctttcattg gacgagccgc
     3061 gagctttaca tcagcttggg cttcaacacc aatctcgctc cgcccgccga agctcccaaa
     3121 actatgaagg acctgctcga accgaaatgg aaaggcaaaa tgaccatcgc cggaaccacg
     3181 acgggcaccc agtggatcgg cgccgtggtc gaggcatacg gccgcgagtt tctggagaaa
     3241 atcgccaagc aagatgtcaa cgtacagaac atttccggcg ctgcgctggc tgagctggtg
     3301 gcgtcgggcg aagtaccgtt gtcgccgacg atcttcaatt cgaacatctc tgtggccaag
     3361 caaaaaggcg cgccggtgga gtggcgtccg atcgatcccg tcgtcgccac caccggaact
     3421 tccggcatgg tcgtcaaagc gccgaatccc catgcagcgc tgttgtttct ggattatctt
     3481 cactccaaag agggacagga ggtggttctg aaaggtgggt tgagctcggc gcgcaaggac
     3541 gtcgcttcgg cggatcaaaa attcaagaaa gtctacatcg agaacaaata tgcaccggac
     3601 gtgatggaaa gaaaatttgc cgagtgggaa ggcctcaaac gtcggctctt catcagcaaa
     3661 cgctgagtcg caaccgtaca agaatgcagc acaaggtcct gatcaccggc gccaacggct
     3721 tcatcggctc ccataccagc agactgctcg agtctctcgg ccattcgatt attccgatcg
     3781 atgtggtagc gcgctcgccg gacctctcgc tcctcggcat caaaacgccg agccatctgc
     3841 tgaacgtcac tgatcagagg gaatttcgcg cgctttgcga acgggaagag cccacgcaca
     3901 tctttcatgc cgcgcatccg ccgcgcgatg agagcccatc cgtaattgac tactgttacc
     3961 acgcgatgac gaacattctc gaagcggcaa aagatctcaa aatcaagcgc gtcgtttatt
     4021 ccagttccgc gtcgatttat ggccaactgc aaaaaccggc tgggagcttg gtcaaagaag
     4081 acgacgcggt gtcgatttat cctacttatt tctatcgcgc cgcgaaaacc gtttccgaat
     4141 ggatgggcga cttctacaaa gaaaagtatg ctgtcgattt tgtcgcgctc cgctattcct
     4201 cggtgtatgg ccccggactg tatcggagca ttccgctgga attgaaaaga ggaatcctgg
     4261 gccaacattg ccgtccgttt ctcacccggc cgaatgatga cttgatctac atcgacgatg
     4321 tcgttgatgc ggtgagccgc gcactcttca ccgataagcg cctcagttgg gcttacaata
     4381 tcggtctgga taagacttat gtgagcgagg atctaaaacg cgccatcgaa aaagccctac
     4441 cccatttgca gttcgaaatt ggcgagcacc cgaatgctgc cgaggtggcg ccgcacaggt
     4501 tgcgcagccc cctcgatatt tccttggctc gacgagagct cgactggaaa ccgaaaatct
     4561 acttggaaga aggcatcgcg cgcctgacgg cttggctgct cgagcataaa aataaactgc
     4621 agcagtgagt cgtcaagcaa cgaggacgac gtgaatcgac gacaacgggc ttggatgctg
     4681 atttgctgcg ccgttcagac gatattgctt gccacgttcg cttgcgcggc caccgcgccg
     4741 atgccgccgg cgcaattagc actctaccag ggcgccgatc gagaaaagat ttttatcgag
     4801 ggggccaagc gcgagggaca gttcactctc tatacctcgc atacctggtt caaaactttc
     4861 gtcggcgaat tcgagaaaaa atatcccttc gtgcgtgttt cgcaatggcg caacgattcg
     4921 aagaacatca tccgcaaagt attggaagaa gcgaaagccg gtcgcgtcct cgccgatatc
     4981 gtcgagacca ccgcggatgg aatgggtctt ttgaggcgcg aaggcttatt ccaggagtac
     5041 tattctcccg aagcgcgcta ttatcccaat gagctgaaag ccaagggtaa aaacggtttt
     5101 ttctatttgc ccaatcgcga aacctacaac agcttaggct tcaacaccaa cctcattcca
     5161 cctgaaactg cgcc
//
LOCUS       DASULZ010000371         4871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_655272, whole genome shotgun sequence.
ACCESSION   DASULZ010000371 DASULZ010000000
VERSION     DASULZ010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655272"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VFY96_07690"
     CDS             complement(<1..966)
                     /locus_tag="VFY96_07690"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5606380.1"
                     /translation="MRVTKLFVLSIVIFAAARMAPAQTIDEIYKKALTEGGTLNFYGT
                     LAQINAETILPAFEKRFSGIKVNHVDATADQLAARVIAEARGGRVLADIFQTLLDGLV
                     HVKDQGLFLDKLYPEAEAFPKELKGTSWVASDLIYIVPAWNTTKVKKEEEPKQFEDFA
                     DPRWKNRIIAEPRDFQLLIGLSRYKYNNDEKAIALLKRIAANNPEFHKGHSQLAELLV
                     AGQAAACLTCYAHHYPIRKRKGAPVDYMLTEGTGDIDATAILKTAPHPNTALLFARWT
                     ASEEGQKIYAQGGRLPAHPKVEPVEKTRPAKVYPVGENDVKDYPKY"
     gene            complement(997..2007)
                     /locus_tag="VFY96_07695"
     CDS             complement(997..2007)
                     /locus_tag="VFY96_07695"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606381.1"
                     /translation="MSRHSRMYFSLAFLLSLQIILADRAAAQELKKVRFGYPSLGFRQ
                     GHIWVARDEGLFQKYGLDIEPIFLRGGQLAIQALAGGDPPLMSIGQVVQAGLAGFDLV
                     LIAGVESYYDSTVFARPGITRLEQLKGKRIGISGFGAATHFAAIILAQHLHLDPDKDM
                     VLVPGGPDAERLAAMSAGKIDAAIFNSSTVPIAKRMGLIELVKIPDLKVEVQGNGMAT
                     TRAYIKTNRDVVKSALKGYIEGINFIFSNKQAAQKVFAKYMRTNDADVLESSYQAYVG
                     TTPKRPYPTLKGLQFLLDRLVPTMPQAKTAKPEQFVDMSFLQQLDQEGFFNEMAKRYP
                     AR"
     gene            complement(2292..3383)
                     /locus_tag="VFY96_07700"
     CDS             complement(2292..3383)
                     /locus_tag="VFY96_07700"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5606382.1"
                     /translation="MAVLLACVLGSSANATAADEAKSGNDWWRVVEAAKKEGQLTVYI
                     SGYEAVLPFFEKEFPEIKLTAVTARGNQLGQRLLSERRAEKYLADVVSSGANPNYQLF
                     YPAKALAPIKAALILPEVLDQTKWYLNKHQYSDPESQYVFNYVGSATYGAVNYNSKLV
                     DIKEFKSYWDLLNPKWKGKIEARDIREAGPGAGNTRFFYYHSELGPPFIRKLFGETDV
                     TLFRDFRQGPDWLATGKFAICFFCDVDVLKQQGLPVDTFGPRVFKEGGGLVQQFGTLS
                     FVDRAPHPNAAKVFINWLLSRRGQIALQKSQANAESPADSLRIDIPKDDVPYQSRRLD
                     GVKYLDTGRPEWIEMKPILDVVNEALRAA"
     gene            complement(3507..4640)
                     /locus_tag="VFY96_07705"
     CDS             complement(3507..4640)
                     /locus_tag="VFY96_07705"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5606383.1"
                     /translation="MKRLLQLFESCAVVVLFLSCAMTYESGAQAKPASHVEWEKTIEA
                     AKKEGQVAVYISGYEEILPEFQKEYPDIKVLPTTGRGSQVGQKLLAERRAEKYLADVV
                     SAGGVTTYQQLFPAKVFDPIKPALLLPEITDASKWYQGKHHYSDPENQYILNYIGTAT
                     YGSISYNTKLVNVKEFKSYWDLLAPKWKGKIISRDVRVAGTGSGNARLFYYLPDVGPS
                     FIKKLYGEMETTLFRDYRQGTDWLAVGKAAICFFCEADVSKAQGLPVDTFGPGVFKEG
                     AGLVTQFGTLGLVNRAPHPNAAKLFINWLLSRKGQMTLQKIMLPTENPVDSLRIDIPK
                     DDVPVLQRRFDNVKYLDTSRPEWQNMKPILDLMNEALKSAGKQ"
BASE COUNT         1077 a         1367 c         1259 g         1168 t
ORIGIN      
        1 gtacttggga tagtccttga cgtcgttttc accgaccgga tagaccttcg cggggcgggt
       61 cttctcgacc ggttcgactt tgggatgcgc gggcaggcga ccgccctggg catagatctt
      121 ttgaccttct tcgctggcag tccagcgtgc aaatagcagc gcggtgttcg ggtgcggcgc
      181 ggttttgagg attgccgtgg cgtcgatgtc gccggttcct tccgtcagca tgtaatccac
      241 cggagcgccc ttgcgtttac ggatcggata atggtgcgcg taacaggtaa ggcacgcagc
      301 cgcctgaccc gcaaccaaga gctcggcgag ttgggaatgg cccttgtgaa actcagggtt
      361 gttcgcagca atgcgcttga gcagtgcaat agccttttca tcgttgttgt acttgtagcg
      421 cgacaggccg atcagtagct gaaaatcgcg gggctcggcg atgatccgat tcttccagcg
      481 cggatcggca aaatcctcga actgttttgg ttcttcctct tttttcacct tcgtcgtgtt
      541 ccatgccgga acgatgtaaa ttaaatcact ggcaacccag ctcgtgcctt tgagctcttt
      601 gggaaaagcc tccgcttcgg gatagagctt gtcgagaaac aaaccttggt ccttgacgtg
      661 caccagaccg tccagcagtg tttgaaaaat atccgcgagc actctgccgc cgcgcgcttc
      721 ggcgatcact cgcgcggcaa gctgatctgc ggtagcgtcg acatgattca ctttgatgcc
      781 cgaaaaacgt ttctcgaagg cgggaagaat ggtttccgca ttgatctgcg cgagagtccc
      841 gtagaaattg agcgtgccgc cttcagtaag tgctttcttg tagatttcgt cgatcgtttg
      901 cgccggcgcc atgcgagccg ccgcgaaaat aacgatcgac aacacgaaca gtttggtcac
      961 tcgcatacat tcctcccgta tagaagctca cggcgattat cgcgccggat atctcttcgc
     1021 catctcgttg aaaaagcctt cttggtcgag ttgctgtaag aaactcatgt cgacgaactg
     1081 ctcgggcttg gccgtctttg cctgcggcat ggttggcaca agcctatcta aaagaaattg
     1141 cagccctttc agtgtcggat acggtcgttt cggcgtcgtg ccgacgtacg cctgatacga
     1201 gctttccagc acgtcggcat cgttggtgcg catatatttc gcgaaaactt tttgcgccgc
     1261 ttgcttattg ctaaaaataa agttgatccc ttcgatgtat cccttcagcg ctgacttcac
     1321 gacgtcgcga ttcgtcttga tatatgcacg ggttgtcgcc atgccgttcc cttgcacttc
     1381 aaccttcagg tcgggaattt tgacgagctc tatcaagccc atgcgcttgg cgatcggcac
     1441 cgtcgatgaa ttgaaaatcg ccgcatcaat cttgccggcg gacatcgcgg ccaagcgctc
     1501 ggcgtcgggc ccgccgggca ccagaaccat gtccttgtca ggatcgaggt gaagatgttg
     1561 ggcgagaata atagcggcaa aatgcgtcgc cgcgccgaaa ccgctgatgc cgatgcgctt
     1621 gcccttcagc tgctccaatc gcgtaattcc cggtcgagca aaaaccgtcg agtcgtaata
     1681 gctctcgacc cccgcgatca gcaccagatc gaagcccgct agtccagcct ggaccacctg
     1741 accgatgctc atcaacggcg gatcgccgcc ggccaaggcc tgaatcgcca gttgcccgcc
     1801 gcgtaaaaaa atcggctcga tatcaaggcc atatttttgg aacagccctt catctctcgc
     1861 aacccagatg tgaccctggc gaaatcccag ggacggataa ccaaaccgca cttttttcag
     1921 ttcctgcgcg gcggcgcggt cggccagaat aatttgcaga cttagaagaa aagcaagcga
     1981 gaagtacatg cgcgagtgtc tcgacatgtt gggctcctct actctatcgg cttgcacaga
     2041 ttcgactacc tctgaattat aaacagatca gtttcaccag attgtcttca taaacttacg
     2101 ctcccccacg gaaaataaac gctatcgaga cgactcacag agctgttatg ctgagcgcag
     2161 cgaagcatct ttttatggct gatagaagaa gcagattctt cggctgcgcc tcatctatga
     2221 catacagata atggctaagg ccgctcgttt catttcgagc cgagggcgag aaatctttct
     2281 tcatctccat cctaagcggc cctcaacgct tcattcacca catctaggat cggtttcatc
     2341 tcgatccact ccggcctacc tgtgtcgagg tatttaacgc cgtcgagacg gcgcgactga
     2401 tacggtacgt catcttttgg aatatcgatc cgtaacgaat ccgccgggct ttcggcattc
     2461 gcctggctct tttgcagcgc gatctgtccg cgccgcgaca aaagccaatt gatgaaaacc
     2521 tttgcggcgt tgggatgagg agcccggtcg acgaaagaca gtgtgccgaa ctgctgcacg
     2581 agaccgccgc cttccttgaa cacgcgcggc ccgaaagtat ctaccggcaa gccctgctgc
     2641 ttgagcacat cgacatcaca gaaaaaacag atggcgaatt ttccggtcgc tagccaatcc
     2701 ggtccctgac gaaaatcccg aaatagtgtc acatccgttt cgccaaagag cttgcgaatg
     2761 aacggcggac cgagctctga gtgataataa aagaatcgcg tattgcctgc gcccggtccc
     2821 gcctcccgga tgtcgcgcgc ttcgattttc cccttccact tgggattcag caggtcccaa
     2881 taagacttga actctttgat gtcgaccagt ttagagttgt aattgaccgc gccgtaggtc
     2941 gcagaaccga cgtaattgaa aacgtattgc gattccgggt ccgagtattg atgtttgtta
     3001 aggtaccatt tcgtctgatc gaggacttcg ggcaagataa gcgccgcctt gatcggcgcg
     3061 agtgctttcg ccggataaaa gagctgataa ttgggattgg cccccgagct tacaacgtcg
     3121 gccaaatatt tctcggcgcg ccgctcgctt aacaagcgct gaccgagctg atttccacgc
     3181 gcagtgaccg cggtgagctt aatctcggga aattccttct cgaaaaacgg cagcaccgcc
     3241 tcatagccgc tgatgtaaac agtgagctgc ccttcctttt tagccgcctc aaccacccgc
     3301 caccaatcat ttcccgactt cgcttcgtca gcggcggtgg cgttagccga agaccccagc
     3361 acgcacgcga gcagcaccgc cacccacttt ggatttcgga ttttagattt tggattgtcg
     3421 gaaaaaatac tcggaaatac gatgggcaga atttttattc ccttccctta atccaaaatc
     3481 caaaatcgaa aatctaaaat attctcttac tgctttcccg ccgacttcag cgcttcattc
     3541 atcagatcca agatcggttt catgttctgc cattccggcc ggctggtatc taaatatttc
     3601 acattatcaa agcgccgttg cagcaccggc acatcgtctt tgggaatgtc gatgcgcagt
     3661 gagtccacgg gattttccgt gggcagcata attttttgca atgtcatttg tcccttgcgc
     3721 gacagcagcc aattgatgaa aagcttcgcg gcgttcggat ggggcgcgcg gttaaccaag
     3781 ccgagggtgc caaattgagt gactaaaccg gcgccttcct tgaacacgcc cggcccgaag
     3841 gtatccaccg gcaggccctg cgccttggaa acatcggctt cgcaaaaaaa gcagatcgcc
     3901 gccttgccga cggcgagcca gtcggtgcct tgacgatagt cgcgaaacag agtcgtctcc
     3961 atttcaccgt aaagtttttt gatgaacgac ggtccgacat caggcaggta ataaaacagc
     4021 ctcgcattgc cagagccggt gcccgcgacg cgaacgtcgc gcgagataat cttgcctttc
     4081 cacttcggcg cgagcaggtc ccaataggac ttaaactcct tcacgttgac gagcttggtg
     4141 ttgtaactga tcgagccgta ggtggctgtg ccgatatagt tgaggatgta ctgattctcc
     4201 ggatcggaat agtgatgctt gccttgatac cacttggaag cgtcggtgat ttccggcagg
     4261 agtaatgcgg gcttgatcgg atcgaaaacc ttcgctggaa agagttgttg gtaagtcgtc
     4321 acgccgccgg cgctaaccac atccgcaaga tatttttccg cgcgtcgctc ggcgagcagt
     4381 ttctgaccca cctgggagcc tcggcccgtc gtcggcagaa ccttgatatc cggatactct
     4441 ttctgaaatt cgggaagaat ctcttcatag ccgctgatgt acacggcgac ttggccttct
     4501 tttttggcgg cttcgatagt cttctcccac tcgacgtgcg aagcgggctt cgcttgcgcg
     4561 ccggattcat aagtcatcgc gcaagacaaa aatagaacga ccacggcgca gctttcgaat
     4621 aattgcaata aacgtttcac agtaatctcc acgttccgca tgtttcacat tctgtcgtcc
     4681 tgaccttggc cgaaaaactc agaatgacct tgcgcatgtc gccattgcta acagagtatg
     4741 ccgatggagg acggctggga tacggccgtc gttttgaacg gcttgaacga tttgaccaat
     4801 taacccgact gtcctgatcc ggccggcggc atatggtagc gcaccgatcc ggctctgccc
     4861 acttctagcg a
//
LOCUS       DASULZ010000372         6192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_350147, whole genome shotgun sequence.
ACCESSION   DASULZ010000372 DASULZ010000000
VERSION     DASULZ010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6192
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350147"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..1187)
                     /locus_tag="VFY96_07710"
     CDS             complement(222..1187)
                     /locus_tag="VFY96_07710"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606384.1"
                     /translation="MLQRFMIVLLSIFLVQSSTTALALETVTAALTSKAFQYVPLVIA
                     QERGYMKEEGLDLKLVYMQNAAGLQALIANQVQFSGSGSSALVAISKGKAPLKTVLAF
                     NDQVLQWLVARPNIKSLRDLNGKKIATTGVASIAAYMLRNILTQKNIPKDIVLIDPGP
                     VNRLPSLLSGAVDAAIVSPEERYAAIDQGMKDIMFFGKEVKNSWGTFATSDRFLKEQP
                     KQLAGFVRAVIKGLKIAREDREGTIAAVAKFSELDKALSTRMYDDLVGTFTKAGYVDE
                     QSQKNDLAIVAQVADVAEVVAPRRAYDFSFARAAEQQLTKQNWKP"
     gene            1361..1987
                     /locus_tag="VFY96_07715"
     CDS             1361..1987
                     /locus_tag="VFY96_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606385.1"
                     /translation="MVLLVLWLLVANIGCSTSTVTPGATLLSGLNQYQSEMQRLGGLE
                     RWPDRQQAAGTLKTVVTATVGSSREFYRLIDLDLRKREFMITMRQSSVGADRLQEMKD
                     ELVKIDEEIAALKPVVRSQVEYSPLQSEAQQPIAGVATLGLLDLAVERFSAGSGSGSD
                     RRSTKIEQYLITDLGTFATVRAPEGQTYRCMTYGMAEEGSSMQCDPVR"
     gene            complement(2092..3036)
                     /locus_tag="VFY96_07720"
     CDS             complement(2092..3036)
                     /locus_tag="VFY96_07720"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606386.1"
                     /translation="MALFLWLPAAFAHAQTDKKISVSLIYEAIAATNATLWLAEDAKL
                     FEKYGLDTKVVAARGAVPVQALVSGSVEFGAFSGSSAIAAVLAGSDLVFVAAKGNFAV
                     MSFWVRRDSPIRTLANLKGKTIGVTRAGSATHSIARIALKSAGLSERDVKFLHHGGLP
                     ESFASLDQGIVDASLGSPPRPGFRELVDLSSLKIPFLQGGIELQRSFLQSRRETVVNL
                     LKAYVETIKLAKEKPELAMISISKRMRVPLDIVRPAYQPHENVLEETPYVRRDSVQVI
                     LDQYPKEQVRNLTYEKLVDNSLLQQLEQSGFIRNLYRK"
     gene            complement(3186..4259)
                     /gene="modC"
                     /locus_tag="VFY96_07725"
     CDS             complement(3186..4259)
                     /gene="modC"
                     /locus_tag="VFY96_07725"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HEX5606387.1"
                     /translation="MLLRCRIPLATFELDVDVSFDSRVTAIFGPSGSGKTTLLDAIAG
                     LREITAGEIEMFGKTLFSSAPRINLPPQRRNLGYVPQEGALFPHLSVRRNILFGSERT
                     TQDAGEKTMSLEHVVEILEIGHLLDRSVTELSGGEAQRVALARAILSRPQLLLLDEPL
                     AALDVGLKERILPYLARVRDEFAIPMIYVTHNVTEVLTLADWVLVIRQGRLVAQGPPS
                     ETLTSSRALRAMPEEELENVFTVEFIDADRATGRSRVRLSSGAEIFVPYVPPPARRDF
                     QVRVRADDILIGTHRPEGISAANILPGTVRAVEILDDQSLVRVDAGELFCVRVTAGAV
                     TRLALGAGSAVFLIIKTRSFQLL"
     gene            complement(4261..4935)
                     /gene="modB"
                     /locus_tag="VFY96_07730"
     CDS             complement(4261..4935)
                     /gene="modB"
                     /locus_tag="VFY96_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEX5606388.1"
                     /translation="MIFSADTWQVTLFSIAVGLASTLLILPFGIALAWLFARKEWPLK
                     SLVETIVLLPLVMPPVSTGLILLKIFGRRSPLGQWLYQRGFEIVFNWKGVLIAMAVMS
                     FPLLVRSVRTSLAEVNPRLEQIAATLGASPVRIFFVITIPLAYKGIVAGALLAFSRAL
                     GEFGATILLAGNIPGRTQTLSLAIYNFVQLGKDADAYVLLGITVALAFSFVWLSEWLL
                     RTRENA"
     gene            complement(4957..5739)
                     /gene="modA"
                     /locus_tag="VFY96_07735"
     CDS             complement(4957..5739)
                     /gene="modA"
                     /locus_tag="VFY96_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009672410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5606389.1"
                     /translation="MNRIWSHVVRIATLASIATAVLLPLGTAACAEEILVSAAASLTD
                     ACNDVGRSYRQKSKDSVRFNFGPSSGLARQIDEGAPADLFFSADLAQMDALEKKSLLE
                     PGTRKNLLSNQLVIIVPADSKLSISTPKDLLKPDVKKIALAEPSSVPVGVYTSKYLAD
                     EGLWEQLKAKVVPVQDVRATLAAVESGNVEAGFVYKTDAAVSKKVKIVYAVPIDKGPK
                     IIYPVAVIKGSKHKTAARDFMSYLQSAAAKNAFNKYGFVVLD"
     gene            complement(5770..6072)
                     /locus_tag="VFY96_07740"
     CDS             complement(5770..6072)
                     /locus_tag="VFY96_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606390.1"
                     /translation="MMRPKRAGRSQRSETSGGKTLEQKAPPCAECGSRETIPVFHTPL
                     SPTQKKLIDTGRAILADREEWEGMTEWYCKSCGCDWSRHSRRFKKPGGVNATRSNR"
BASE COUNT         1386 a         1690 c         1778 g         1338 t
ORIGIN      
        1 gacgcaagca ttcaacgccc cgtcccagcc ctgatagtgc ttggggtcga catgatttaa
       61 accgatatga atggaaacgc cttgcgccat gaaagcctcc tctgaccgga agttcaggcg
      121 aagttttaag gcttgcgatt cgacctgtca acaaaaaacc taatgcggag aaactctttc
      181 tcagaagctc gtaaaatttc tcttagtcga tcattttttc tctacggctt ccaattttgt
      241 ttagtgagtt gctgctcagc cgctcgggcg aaactgaaat cgtacgcgcg ccggggtgct
      301 acgacctcgg cgacatcggc aacctgggca acgatcgcga ggtcgttttt ttggctttgc
      361 tcgtcgacgt aaccggcttt tgtgaaagta ccgaccaggt cgtcgtacat gcgcgtcgaa
      421 agagctttgt ccaattcgct gaacttggcg acagcggcaa tggtgccttc gcgatcttca
      481 cgagcgattt tgagaccctt gataacggcg cggacgaatc cggcgagttg tttcggctgt
      541 tccttgagaa accggtcaga ggtcgcgaag gtgccccagg agtttttcac ctctttgccg
      601 aaaaacatga tgtctttcat gccttggtcg atagcggcat agcgttcttc cggactgacg
      661 atggccgcat cgacagcgcc ggataacagc gacggcaggc gattcaccgg tccgggatcg
      721 attagtacga tgtccttggg aatgtttttt tgagtcaata tgtttctcaa catataggcg
      781 gcaatcgaag cgacgccggt ggtggcaatt tttttaccgt tcagatcacg caagctcttg
      841 atgttaggac gcgcaaccag ccattgaagc acctgatcat tgaaagcgag aacggttttg
      901 agcggcgcct tgcccttgga aatcgccacg agggcgctgc tcccggagcc ggagaactga
      961 acctgattgg cgatcaaagc ctgaagaccc gcggcattct gcatgtacac gagcttgaga
     1021 tcgagccctt cttctttcat gtagccgcgc tcctgggcga tcaccagtgg aacgtactgg
     1081 aaggccttgg aggtgagggc ggcggtgacc gtctccagcg caagcgctgt tgtggacgat
     1141 tggactagaa aaatcgagag caacacgatc ataaaacgct gcagcatccc tctatcctcc
     1201 gtgagataac tcgttcgctc gcagcctctt tatgtctcaa caacaaattg ttctcaagcg
     1261 ctactgaagc ttcagtttta gtcgaagcac cgcggctggc gcgcggaaat ttgcttcagg
     1321 gacatttgcc gatgtgatat cgtctacgac catgaagaaa atggtactgc tagttctgtg
     1381 gcttctggtc gcgaatatcg gttgttccac ctcgaccgtc acgccgggcg cgacgctttt
     1441 gtcaggcctc aatcaatatc agagtgaaat gcagaggctc ggcggtctgg agcgttggcc
     1501 ggaccggcaa caggccgcag ggacattaaa aaccgttgtc acggccactg taggcagttc
     1561 tagggaattc tatcgcttga tcgatctcga tcttagaaaa agagaattca tgattaccat
     1621 gcggcaaagc tcggtcggtg ctgatcggtt acaagagatg aaagacgagt tagtgaaaat
     1681 cgacgaagag atcgccgctt taaaacctgt cgtgcgctcg caggtagaat actcgccatt
     1741 gcaaagtgaa gcgcaacaac cgattgcggg cgtggctact ttaggcttgc tggatttagc
     1801 cgtggaacgc ttctcggcgg gcagcggaag cgggtcggac cgacggtcga ccaagatcga
     1861 gcagtacctg atcacggatt tgggcacatt cgcgaccgtg cgggcgccgg aggggcaaac
     1921 ctatcgctgc atgacgtacg gcatggccga ggaaggctcc agcatgcagt gcgacccagt
     1981 caggtgaacg ctgctttcaa gtcttccgcc gccgagacct taccagcagc tcgccgcttc
     2041 gcacgagact aagacacgcc attactgctc agacgttgtt cctggggcag atcattttcg
     2101 gtacaggttc cggatgaatc cggattgttc gagctgttga agtaagctgt tgtcgaccaa
     2161 tttttcataa gtcaggttcc ttacctgttc cttcgggtac tgatcgagaa tgacttgaac
     2221 cgaatcgcgg cgcacgtagg gagtttcttc gaggacgttt tcatgcggct gatatgcggg
     2281 acgaacgatg tcaaggggca ctctcattcg cttggaaatc gatatcatcg cgagctcggg
     2341 tttttctttg gcgagcttga tggtctcgac ataggcttta agcagattga ctaccgtttc
     2401 gcgccggctc tgcaggaagc tgcgctgcag ctcgatgccg ccctgcagaa atggaatctt
     2461 gagcgaagat aaatcgacca gctctcgaaa gcccggacgt ggcggcgatc cgagacttgc
     2521 atctacgatc ccctgatcca gggaggcgaa ggactccggc aatccgccgt ggtgcagaaa
     2581 tttgacgtcc cgttcgctca ggcctgctga cttcaaggcg atgcgcgcga tgctatgggt
     2641 cgcagagccc gcgcgcgtga cgccaatcgt cttacctttc agattggcca gcgttcttat
     2701 cggtgaatcg cgtcgaaccc aaaacgacat caccgcgaag ttgcctttcg ctgcgacaaa
     2761 aaccagatcg cttccggcga gcacggcggc aatggccgag gagccgctga aggcgccgaa
     2821 ttcaaccgag ccgctgacga gagcctgaac ggggacggcg ccgcgcgccg ccacgacctt
     2881 cgtgtcgaga ccgtattttt cgaatagttt cgcatcttcg gcgagccaga gcgtggcatt
     2941 ggttgcggcg atcgcttcgt aaatgaggct gacggaaatc tttttgtcgg tctgcgcatg
     3001 ggcaaacgcc gcgggcagcc acagaaagag cgccatcaca gctaggtggt gatttctcat
     3061 cttggcaatt gtaatgtccg cgcctcccga atcaggtgtt tgcccgccga ctccgcgaaa
     3121 ccgcgaaatc gcgtgcgccc aatctggcgc ggaccaattc gggtgtcaag caactcatca
     3181 agtgattaca gcagctgaaa ggaacgtgtt ttgatgatca gaaaaacggc ggaccctgca
     3241 ccaagagcaa gccgcgtaac ggcgccagcc gtgactcgca cgcagaaaag ttcgccggca
     3301 tcgacgcgca ccaatgattg atcatcaagg atctcgacgg cgcgaaccgt gcccggcaag
     3361 atgtttgcgg ccgagattcc ttcgggccgg tgcgtgccaa tgagaatgtc gtcggcgcgc
     3421 acgcgaacct gaaaatctcg ccgagctggc ggcggcacat agggtacaaa aatttccgcc
     3481 ccggacgaga gccgcactcg cgatcggcct gtagctctgt cggcgtcgat aaactctacc
     3541 gtaaagacat tttccagctc ctcttcgggc atcgctctga gggcgcggct cgacgtaagc
     3601 gtctcgctcg gcggcccttg agccaccaaa cgaccctgcc gaatcaccag gacccaatcg
     3661 gccagggtga ggacttcggt gacgttatgg gtcacgtaga tcatcgggat cgcaaattca
     3721 tcgcggacgc gcgcgaggta aggcaagatc ctttctttga gaccgacgtc gagagccgcc
     3781 agcggctcgt ccaatagcaa caactgggga cgagacaaga ttgcgcgagc cagcgcaacc
     3841 cgctgcgctt cgccgcccga aagctcggtc acagaacgat caagtaagtg gccgatctcg
     3901 agaatttcga ccacgtgctc caggctcatc gtcttctcgc cagcgtcctg ggtggtgcgc
     3961 tccgagccaa agagaatatt tcgccgcacc gaaagatgcg gaaacaatgc gccctcctgg
     4021 ggcacatatc cgaggtttcg gcgttgcggt ggaagattga ttctcggcgc cgacgaaaaa
     4081 agcgtcttac cgaacatttc gatttcgccc gcggtgattt cgcgcaaccc ggcgatggcg
     4141 tcgagcaacg tagtttttcc ggagccggat ggtccgaaaa tcgcagtgac acgggaatcg
     4201 aatgacacat cgacgtcgag ctcgaatgtc gctagcggta ttcggcagcg caataacata
     4261 ttatgcgttc tcccgcgtcc gtagcagcca ttccgagagc catacgaacg agaatgccaa
     4321 tgcgaccgtg atcccaagca aaacgtaggc gtcggcgtct ttgcccagtt gaacgaagtt
     4381 atagatcgcc agcgacagcg tctgcgttcg gccgggaata tttccagcga gtaaaatcgt
     4441 cgcgccgaac tcgccgaggg cacgggaaaa tgccaacagt gcgccggcga cgatgccttt
     4501 gtaggcgagc gggatcgtga tcacgaagaa aattctcacc ggtgaagcgc cgagggtagc
     4561 ggcgatctgc tccagccgcg gattcacttc tgctaacgat gtgcgcaccg agcgcaccag
     4621 caagggaaaa gacatcaccg ccatggcgat gagcacgcct ttccaattga agacgatttc
     4681 gaaacctctt tggtagagcc attggccgag ggggctccgg cggccgaaga ttttcagaag
     4741 aatcaagccg gtcgataccg gcggcatgac cagcggtagg aggacgatcg tttcaacaag
     4801 gcttttgagc ggccattctt tgcgcgcgaa cagccaggcc agcgcaatac caaacggtaa
     4861 aatcagcaag gtgctcgcca gcccgacggc aatggaaaac agcgtgactt gccaggtgtc
     4921 ggctgagaaa atcacggatg aacaagttcc ttgtcctcag tccaatacga caaagccgta
     4981 tttgttgaaa gcattttttg cagcggcgct ctgaagatag ctcatgaagt cgcgggcggc
     5041 cgttttatgt ttcgatcctt tgataaccgc gacggggtaa ataatcttag ggcctttgtc
     5101 gatcggcact gcataaacga ttttgacttt cttggaaacg gcggcatcgg ttttgtaaac
     5161 gaatccggct tcgacgttgc ccgattccac ggccgccaag gttgcgcgaa cgtcttgcac
     5221 cggtacgacc ttggctttca gctgctccca aagaccttcg tcggccaggt atttactggt
     5281 ataaaccccg acgggtacgg aggatggctc ggcaagggcg atttttttga catcgggctt
     5341 cagcaagtct ttcggcgtcg agatcgagag cttcgaatcc gccggcacga tgatcactaa
     5401 ttgattggac aaaagattct tgcgcgtgcc gggctcaagc aagcttttct tctcgagcgc
     5461 gtccatctga gcgagatcgg cggaaaaaaa caaatccgcg ggggcgcctt cgtcgatctg
     5521 ccgcgccaag ccgctggacg gaccgaagtt gaagcggacg gaatctttgc ttttctggcg
     5581 atatgatcgt ccgacgtcat tgcaagcatc ggtcaggcta gcggccgcgg acacgaggat
     5641 ttcttcggcg cacgccgccg tgccaagcgg caatagaacc gcagttgcga tggatgccag
     5701 agtcgcgatt cttacaacgt gcgaccagat tcgattcatc catcctcggg agaaaagcgg
     5761 cagattcggc tagcgattcg accgtgtggc gttcacgccg ccgggctttt taaatcggcg
     5821 cgagtgacgg ctccaatcgc aaccgcagga tttgcaatac cactcggtca tgccttccca
     5881 ctcttcgcga tcggcgagaa tagcgcggcc ggtatcaatc aattttttct gagtcggact
     5941 gagaggcgta tgaaagaccg gaatcgtttc gcgggagccg cattcggcgc acggcggagc
     6001 cttttgctct aaggttttcc cgccggacgt ttcgctgcgc tgcgagcggc cagcgcgttt
     6061 cggacgcatc atttgaggta gccccgctcg cggtagaacc gccgcgctcc gggatgcagt
     6121 ggcacactga acggcgcttc ttcatcattg gcacagagct gacgcatgct gatcggtttg
     6181 aaattgtcgc tc
//
LOCUS       DASULZ010000373         2632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1162742, whole genome shotgun sequence.
ACCESSION   DASULZ010000373 DASULZ010000000
VERSION     DASULZ010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162742"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..1081
                     /locus_tag="VFY96_07745"
     CDS             299..1081
                     /locus_tag="VFY96_07745"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX5606391.1"
                     /translation="MTADTTPGYDLLYDIIRRRSSVRKLKPDPIPDGYIDKILEAGRW
                     AMSGANSQPWEYIVVKDPKVKKDLFRCYSEDNTDFIYWMEQQRVFELRHPAYQMTADE
                     AVERQRRGAGWSEAPALIVVVGDGRRQWGTVQGAHTFGRDQSHLTDGLANTCTFMHLA
                     AVSLGLATQWVTIHVQEPFKKVLGVPDLMMLYLIIPIGYPAVQPSEGVRRPLEEIVHH
                     DHYEQDKFMSNERVIQFLYELRGKTLQTYRQSYVGKEEPKKG"
     gene            1362..>2632
                     /locus_tag="VFY96_07750"
     CDS             1362..>2632
                     /locus_tag="VFY96_07750"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX5606392.1"
                     /translation="MAVDPITVEIIRCALKAAANEMSAVLKRTAYNMMIYEVQDYCVS
                     IVDHQGRTLSQNEGALPIFLADLGVAVQDGVEIYGLDEIHAGDVFLVNHPEICGQHLN
                     NMVVYTPFFWEKKLLCFLAVRAHWIDVGGGSTGFGSSNTRDVYEEGLQVRSVKIYKEG
                     RPNTEVLRLIEDNIRFPESSLGDLRAQIACCRTGEERLNQICRKYGGKVFLQAVETIW
                     EQTDKLVREAVRAIPDGDYEASSFLDDDGRVMNKTIPIKVKVAVRGDELTIDFSEVAD
                     QVPGFINCGSSGGMAAARVAFKALTAPHREVNEGSFRALKVILPAGKLLSARRPAPIG
                     GWSWSLPTVLDTIFRALAPALPDRVPAAHKGDMGGYAIFGLNSKTGRRYVCQNIIGGG
                     WGGRPSEDGASAAVSMCQGDVKNTPIELQEL"
BASE COUNT          565 a          683 c          825 g          559 t
ORIGIN      
        1 gcgccggaga aatcgaggag aaatttctgt cattggccgc gcccgccgtc ggagacgagc
       61 gggcattgct tgttcaaaaa gctattgcat cgttggatga acggcaatcg ctcgagggtg
      121 tgctggcagc gcttgcggac cgagacgaat ccggcgcgta aggtcatcct gagcccttta
      181 ggcaaggatc tagattcttc gctttcgctc agaatgacgg agcatagagc tgtaattgaa
      241 gggtgtgggt gagatataag tattttgtca gaagtggcgt tttctttgga ggctcaatat
      301 gaccgcagac accactcccg gttacgatct tctctacgac atcattcgac gccgttcgag
      361 cgtgcgcaag ctcaaaccgg atcccattcc cgatggctat atcgataaaa ttctcgaagc
      421 agggcgctgg gcgatgtcag gcgccaactc gcaaccgtgg gagtacatcg tcgttaaaga
      481 tcccaaggtg aaaaaggatc tgtttcgctg ctattccgaa gacaatacgg atttcattta
      541 ttggatggag cagcaacggg tcttcgagct gcgccatccg gcttatcaaa tgaccgcgga
      601 cgaggcggtg gagcgccagc ggcgcggcgc cggctggtcg gaagcgcccg cgctgattgt
      661 ggtcgtaggc gacggccggc gccaatgggg cacggtgcaa ggcgcgcata ccttcggccg
      721 cgatcagtcg catctcaccg acggtctggc caacacctgc acgttcatgc atctggcggc
      781 ggtgtcgctc gggttggcaa cgcagtgggt cacgattcac gtccaagagc cgttcaaaaa
      841 agttctcggg gtgccggatt tgatgatgct ctacttgatc attcccatcg gatatccggc
      901 ggtgcaaccg tccgagggcg tgcgccggcc gctggaagaa atcgtccatc acgatcatta
      961 cgagcaggac aaattcatga gcaatgagcg ggtgattcag tttctctacg agctgcgcgg
     1021 gaagacgttg cagacatatc ggcagtctta tgtggggaaa gaagagccaa agaaaggctg
     1081 aaagttcggc tatagaagtg atccacaatg ttaactaaac ggtttggcgc agcagccgcg
     1141 ggctttatcg ggggcgggct cctgagtatg attcttctcg cgttgatggt ttcgatcttc
     1201 gagctggcgt ttcaaagtgt gatgccagca gctttgatcg taggatcgat ttgcggtgtt
     1261 gtgggtttct gctttcctgg aataggagct agcttgtttg cgatggtgga ctggtgacaa
     1321 ggacattgag caagccaaaa gaatcgactg atggaaaagt tgtggccgta gatcccatca
     1381 ccgtagaaat tatccgctgc gcgcttaaag ccgccgccaa tgagatgtcg gcggtgctga
     1441 agcgcaccgc gtacaacatg atgatctacg aggtgcagga ttactgcgtg agcatcgtcg
     1501 atcatcaagg ccgcacgtta tcgcaaaacg agggcgcgct gccgatcttt ctcgccgatc
     1561 tcggcgtcgc cgtgcaggat ggcgtggaaa tttacggctt ggacgagatt catgcgggcg
     1621 acgtgtttct ggtgaatcat ccggaaatct gcggccagca cttgaacaac atggtggtgt
     1681 atacgccgtt tttctgggag aagaagctgc tctgcttcct cgccgtgcgg gcgcactgga
     1741 tcgacgtcgg cggcggcagc accggcttcg gttcgagcaa tacccgcgac gtttacgaag
     1801 aaggactgca ggtgcgctcg gtaaaaattt ataaagaagg ccgaccgaat acggaagtgc
     1861 tacggttgat cgaggacaat attcgctttc ccgaatcttc gctcggcgat ctgcgcgcgc
     1921 agatcgcttg ctgccgcacc ggcgaagagc gtttgaatca gatttgccgg aaatacggcg
     1981 gcaaagtttt ccttcaggca gtcgaaacca tctgggagca aaccgacaag ctcgtgcgcg
     2041 aagcggtgcg ggcgatccct gacggcgact acgaagcctc gtccttcctc gacgacgacg
     2101 gcagagtgat gaacaagacg attccgatca aagtcaaagt tgccgtgcga ggggacgagc
     2161 tgactatcga tttcagcgaa gtcgccgatc aagtgccggg gttcatcaat tgcgggtcga
     2221 gcggcggcat ggcggcggcg cgggtggcgt tcaaggcttt gaccgcgccg caccgcgaag
     2281 tcaatgaagg gtcgtttcgc gcgctcaaag tgattcttcc cgcgggcaag ctgctgagcg
     2341 cgcgccgtcc cgcgccgatc ggcggctgga gctggtcact gccgaccgtg ctcgacacga
     2401 tcttccgcgc gctggcgccg gcactgccgg accgagtgcc tgccgcgcac aaaggcgata
     2461 tgggcggtta cgcgatcttc ggtctgaatt cgaaaaccgg gcggcgctac gtctgccaaa
     2521 atatcatcgg cggcggctgg ggcggccgcc ccagcgagga cggtgcttct gccgctgtct
     2581 ccatgtgcca gggggatgtg aaaaatacgc cgatcgaatt acaggagctc ta
//
LOCUS       DASULZ010000374         3393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1060057, whole genome shotgun sequence.
ACCESSION   DASULZ010000374 DASULZ010000000
VERSION     DASULZ010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060057"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..886
                     /gene="tolQ"
                     /locus_tag="VFY96_07755"
     CDS             146..886
                     /gene="tolQ"
                     /locus_tag="VFY96_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEX5606393.1"
                     /translation="MSFLWPLQLLAQDGIAVPQQHSVLDLVRGSGLVVQAILYLLILF
                     SVISWAIIAQKYRQIRRAKRESERFIEIFWERRNLSSIHDASRELSASPVGQVFRSGY
                     EELVRVSRSKKESSVSDNLTTELSGVDNVSRAMKRATSVETTKMEKNCSFLATTASSA
                     PFVGLFGTVWGIMDAFRGLSVTHSSSIQAVAPGIAEALIATAVGLAAAIPALMAYNYF
                     VQQIKFIAVEMDNFQHEFLNIAERHFFK"
     gene            1063..1479
                     /gene="tolR"
                     /locus_tag="VFY96_07760"
     CDS             1063..1479
                     /gene="tolR"
                     /locus_tag="VFY96_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HEX5606394.1"
                     /translation="MAVDASSPREGTTISQINVTPLVDVMLVLLVIFMVTAPIIQQGV
                     QVNLPQTKASAIAGSEEPLIVTVAKDGKIYLNDNVIGLADLGTKLTAIRKLQADKQVY
                     LRADQDVRYGTVMKTIAEIKQAGIEKLGMVSRPPSE"
     gene            1596..2426
                     /locus_tag="VFY96_07765"
     CDS             1596..2426
                     /locus_tag="VFY96_07765"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX5606395.1"
                     /translation="MHLALIAGLFLAPFLPSRERPAPPVYVVDLVGGEKIGGLKLGTE
                     ISAPASPKPKTEPIKPALPEPKKEARELKSEKTEKTKPVEKKAPEEKVVALKEKAKTE
                     PPVVKKEASKELKEEAKSEIASADSVRERLLQSALDRAKSKAETSQKPAKGEILSSGA
                     GEGEGAAALGQGGRGGPGIVKGMDFIIYQNRMLSTIKENWAWVGQRSNLKVVVHFGIK
                     DSGEIVGLKIVQPSGDPSYDESVLRAVKKSSPLSPPPDSYRKDFADVELTFRPRDLGA
                     "
     gene            2428..>3393
                     /locus_tag="VFY96_07770"
     CDS             2428..>3393
                     /locus_tag="VFY96_07770"
                     /inference="COORDINATES: protein motif:HMM:NF015980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606396.1"
                     /translation="MMRSRSLLLLICLLFSATKIAHGAVTGEIVGQGGQRFPIAVSPL
                     KNIGANTADSGRVSTGIADAMVHDLELSGWFRVLDRSAYIENAQSGITLGTFDFKDWS
                     TIGAEGLVKGGFSIQGDEITVELRLFDVYQNKERIGKRYTGRVRDFRRIAHKFVDEII
                     NQFTGVPGVFNTRIAYVSNSGGRFKEIFVSHLDGSEKFQVTDNHTINLSPAWSPDGRS
                     VLYSSFKDRGQTLYLFELYSGKEIKFTPRGGGRYLGGKFSPDGQSIVATLETGGNTNL
                     YLLDRSGNVIRQLTSDAGIEVSPAWSPDGKQIVFVSDRSGSPQIYI"
BASE COUNT          851 a          851 c          930 g          761 t
ORIGIN      
        1 ccgccttgcc gcttaaatcg aggtcgccga gcttgcgaat catacaacct tttcgactgc
       61 caattttatg atgaagttag ccattcttaa ttgactaatt acaagggttg taatactttt
      121 tcaaacagct ccattaaaac acgtgatgtc ttttctctgg ccgcttcaac tgttagccca
      181 agacgggatc gcggtaccgc aacagcatag cgtccttgac ctggttcgcg gttcaggcct
      241 tgttgttcag gcgattctct acctgctgat cctgttttct gtaatcagct gggcgatcat
      301 tgcgcaaaaa taccgccaaa ttcgccgcgc gaaacgtgaa tcggaaaggt ttatcgaaat
      361 tttctgggaa agacgcaatt tgtcgtcgat tcacgacgcc agccgcgagc tgagcgcgag
      421 cccggtgggg caggtcttcc gctccggtta cgaagaactg gtgcgtgtct cgcgatccaa
      481 gaaagaatcc tcagtgagcg acaatcttac aaccgaactc agcggggtcg acaacgtttc
      541 gcgcgccatg aagcgcgcga ccagcgtcga aacgaccaaa atggaaaaga actgttcctt
      601 tctcgccact accgcaagct cggcgccgtt tgtcggatta ttcggcaccg tctggggcat
      661 tatggatgcc tttcggggct tgagcgtgac tcactcctca agcattcaag cggtggcgcc
      721 cggcattgcc gaagctttga ttgcgacggc agttggcctg gcggcggcga ttcccgcgtt
      781 aatggcttat aactatttcg ttcagcaaat taagttcatt gccgtcgaga tggataattt
      841 tcagcatgaa tttctcaaca tcgctgaacg tcacttcttc aagtaattag agaaccgagt
      901 ataatcaaag gttgttcggg cggtttgaga gcgagtggag cgcgtgactt tcgccccaca
      961 cgttccgtag ctcagcgaaa ccgagggtat ggttgaacgg gctcctcgca cgtcaaagct
     1021 aataccagat atgcgtttag cctcgtaaca caagaggtaa ttatggccgt agatgcgtct
     1081 tcgccgcgag aaggaaccac aatctcgcaa ataaatgtta cgccactcgt ggacgtcatg
     1141 ttggtacttt tggtcatttt catggtgacg gccccgatca tccaacaagg agtccaggtc
     1201 aacttgccgc agaccaaagc gagcgccatt gccgggagcg aagagccgct gattgtgaca
     1261 gtggcgaaag atggcaagat atacctcaac gacaatgtta tcggcttggc ggatcttggg
     1321 acgaaactga ctgcgatccg aaaattacaa gcggataaac aggtttattt gcgagcggat
     1381 caggatgtac gttacggcac ggtgatgaaa acgattgccg aaataaagca ggcgggaatt
     1441 gaaaagctag gaatggtcag ccgaccgcct tctgaataga gatgaccccc acgacaggaa
     1501 aagcctcgac atctacgatc agtttttggg agcggccgtt tcctgccagc gggagaacgg
     1561 agccgcttcc taggtggttt ttcgtttcat tgatcgtgca tctcgcgtta attgcaggtt
     1621 tatttctcgc tccattcctg ccatcccgcg aacgtccggc accgccggtt tatgtggtcg
     1681 atctcgttgg cggagaaaaa atcggcggcc tgaagcttgg tacggaaatc tctgcccccg
     1741 cctcaccgaa gcctaaaaca gagccgatta agccggcgct acccgagccc aaaaaggaag
     1801 ctcgggagct caaatctgaa aaaaccgaaa agaccaagcc ggtggaaaaa aaagctccgg
     1861 aagaaaaggt cgtcgcatta aaagaaaaag cgaagacaga accaccggta gtcaaaaaag
     1921 aagccagtaa agagctaaaa gaggaggcaa agtcagagat tgcttcggcg gacagcgtgc
     1981 gggagcgact tctgcaatcc gcgttggatc gcgctaaatc caaggcggaa acgtcacaaa
     2041 agccggccaa aggggaaatc ctcagttcag gtgcgggaga aggggaaggc gctgccgcgc
     2101 tcgggcaggg cggacgaggt gggcccggta tcgtgaaagg tatggatttc attatttatc
     2161 agaaccgaat gctcagcacg atcaaggaaa attgggcatg ggtcggacag cgcagcaatc
     2221 tgaaagtagt cgtgcatttt ggcatcaagg atagcggcga aattgtcggg cttaagattg
     2281 tgcagccgtc gggggacccc tcatatgatg aatcggtgct tcgcgcagtg aaaaaatcaa
     2341 gtccgctgtc accgcccccc gacagttatc ggaaagactt tgccgacgtc gagttgacgt
     2401 tccgcccgcg ggacttagga gcttaggatg atgagatcgc gaagtttact tctactgatt
     2461 tgcttgctgt tcagcgccac aaagatagcg cacggtgcgg tcacaggtga aatcgtgggc
     2521 caaggaggcc agagatttcc gatagccgtt tcgccgctaa aaaatatcgg cgcgaatact
     2581 gctgattccg gtcgagtttc taccggaatt gccgacgcga tggttcacga tctcgagcta
     2641 tccggctggt tcagagtctt ggatcgttca gcttacatcg agaatgcgca aagcggcatt
     2701 acgctaggaa catttgactt taaggattgg tcgaccatcg gggccgaagg tctggtcaag
     2761 ggcggcttct caattcaagg agatgaaatt actgtagagc tgcgcttgtt cgatgtttac
     2821 caaaacaagg agcggatcgg taaacgttat accggccgag tgcgagattt tcgccggatc
     2881 gcgcacaagt ttgtcgacga gattatcaac caattcaccg gggtcccggg ggtcttcaat
     2941 accagaatcg catacgtttc caatagtggc gggcgcttca aagagatttt cgtctcccac
     3001 ctcgacggca gcgaaaaatt tcaggtcacc gacaatcaca ccatcaattt gtctcccgcc
     3061 tggagtccgg acggtcgctc ggttctctac tcctcgttca aggaccgcgg ccagactctt
     3121 tacctgttcg agctttacag tggcaaggag attaaattta ctccgcgggg tggcggcagg
     3181 tacctcggcg ggaaattttc tccggatggt caaagcatcg tggcgaccct cgagaccggc
     3241 ggcaacacca acctttatct gctggatcgc agcggcaatg tgattcgcca actcaccagc
     3301 gacgccggaa tcgaagtttc gccagcgtgg tcgccggacg gcaaacaaat cgtgtttgtg
     3361 tcggatcgaa gcggttcgcc acaaatatac att
//
LOCUS       DASULZ010000375         2566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_147653, whole genome shotgun sequence.
ACCESSION   DASULZ010000375 DASULZ010000000
VERSION     DASULZ010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147653"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..366
                     /locus_tag="VFY96_07775"
                     /pseudo
     CDS             101..366
                     /locus_tag="VFY96_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184361.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            492..1910
                     /locus_tag="VFY96_07780"
     CDS             492..1910
                     /locus_tag="VFY96_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5606397.1"
                     /translation="MSPNFICAIEANARRFAEKTAVVCDENKLTWSELHGFASGFAGH
                     LSDQGVIPGDRVAILLPNGINFVIAFLAILKLGATPAPLNPQIKEDELAAFRTDLRPK
                     LSVDQVVMSRSTWRTVEQASAPSLILYTSGSTGRPKGAVFSHDALTFANHSWAEPVMG
                     LTPDDVVLVVVPLAHSLGLNGGLLAPLLTGATVVILERFAPEAVFSAVENNHVTVLPA
                     VATIFRRLLNSPAFTDSDFSSLRLAVSGAAPCPWELAAEWREKTSTRIVRGYGMTELF
                     RPLSFRCAESTDVPNAVGKPLPGVEIRLDIDAANSDDIGELWIKSPAMMDSYLNAPDE
                     TAEVISAGWFRTGDVATISPEGYVQIVGRVRERILRGGYSVFPQEIEAVLSSHPAVAE
                     AAVVAVPDADLGEEVAAFVSLKPTFNVTADGLLAHCRDHLARYKYPRQLLILPELPKG
                     PTGKILKSALIAQAQANKDFSL"
     gene            1923..2216
                     /locus_tag="VFY96_07785"
     CDS             1923..2216
                     /locus_tag="VFY96_07785"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX5606398.1"
                     /translation="MIHEIVIQHVLPEQRDEYVKVFGDFLKKASYPGAHGIRFFASIE
                     DPGRVILMIDWDSVEAHTQIRGTPTYNAMRELTGKYQTAKSDGGHFILHEVSR"
     gene            2365..>2566
                     /locus_tag="VFY96_07790"
     CDS             2365..>2566
                     /locus_tag="VFY96_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606399.1"
                     /translation="MKHALLVILCSFLWTHSANAQAPFYQGKTIRVIVGTPPGNLYDF
                     WARLIAEHLGKHIPGNPIFVVQN"
BASE COUNT          602 a          684 c          689 g          591 t
ORIGIN      
        1 atttcgcctg gattcccgct aaaagcatgc gggaatgacg agatttgcgc agcagacgag
       61 tggaaattca accaatgagt cgtaagcaac cggcgtttat attctcgcca gaaaaagaaa
      121 cggtacactg tacatcggag taaccagtga tctgctaaag cgatgttggg aacacaagaa
      181 taatttggtt gaagggttta gaagcaatat ggtgttcatc aactggcgta tttcgaatta
      241 catcaagaga tgaccgaagc aatagcaaga gaaaaacagt tcaagaaatg gaaccgcgcg
      301 tggaagctga aactgatcga aaaacacaat ccagactgga gagatttgtg ggaagatatt
      361 gcctagatga taatgaaaca atcgtcagct acgtcacccc cgaaagcctt tatcgggggt
      421 ccagttccga ttatctggat tcccgctaaa aacctgcggg aaaatgacga ctcgtgagct
      481 aagctacaat cgtgtcaccg aatttcatct gcgccatcga agccaacgcg cgccggttcg
      541 ctgagaaaac cgcggtagtt tgcgatgaaa ataagctcac ctggtcagag cttcacggct
      601 tcgcctccgg ttttgccggg cacctttcag atcaaggcgt aattcccggt gatcgcgtcg
      661 cgatccttct gcctaacggc attaatttcg tcatcgcctt tttggcgatt ctcaaactcg
      721 gcgctacgcc ggcgccgctc aatccgcaaa tcaaagagga cgagctcgcc gcatttagaa
      781 cggacctcag gccgaagttg agcgtcgatc aggttgtgat gagtcggagc acgtggcgca
      841 ccgtcgaaca agccagcgct ccatcactga ttctatacac gtccggcagc acgggccgac
      901 ccaaaggcgc cgtgttcagc catgacgccc tgacgttcgc caaccattcg tgggccgagc
      961 cggttatggg gcttactcca gacgacgttg tgctggtggt ggttccactg gcgcattcct
     1021 tggggttgaa cggcggtttg cttgcaccgc tcctgaccgg tgcaaccgtc gtgattcttg
     1081 aacgcttcgc tcctgaagct gttttttcgg ctgttgaaaa caatcacgtg accgtcttgc
     1141 cggccgtcgc cacgatcttt cggcgcttgc tcaattcgcc ggcgtttacg gatagcgatt
     1201 tctccagctt gcgtctggcg gtttctggcg ccgcgccttg cccgtgggag ttggcagccg
     1261 aatggcgaga gaaaacttcg acgcgcattg tccgtggcta cggcatgaca gaattatttc
     1321 gtccgctctc ctttcgctgc gccgagagca ctgatgtccc caatgcggtc ggcaagccac
     1381 tgccaggcgt ggaaatacga ctcgatatcg acgccgcaaa cagcgacgac atcggcgagc
     1441 tctggatcaa atcgccggcg atgatggatt cttatttaaa cgctcctgac gaaactgctg
     1501 aagtcatttc cgccggttgg ttcaggaccg gcgatgttgc gacgatctcg cccgagggtt
     1561 atgtgcagat cgtcggccga gtgcgcgaac gtattctacg cggcggatat tccgtctttc
     1621 cccaggagat cgaagcggtg ctgagctctc acccggcggt cgccgaagcg gcggttgtcg
     1681 ctgttccgga cgcagatctt ggtgaagaag tcgcggcttt cgtcagtttg aaaccgacct
     1741 tcaacgtcac agcggatgga ttactcgccc actgccgcga tcaccttgct cgctataaat
     1801 atccccgtca acttctaatc ttgcctgagc ttccaaaagg gccaacggga aaaattctta
     1861 aatcagccct gatcgctcaa gcccaagcga ataaagattt ctcgctttga aggaggccac
     1921 cgatgatcca cgaaattgtg attcaacacg ttctgccgga acaacgagat gagtatgtca
     1981 aagtttttgg cgatttcttg aaaaaggcga gctatccggg cgcacacgga atcagatttt
     2041 tcgccagcat cgaagatccg ggacgggtga ttttaatgat cgattgggac agtgtggaag
     2101 cgcacacgca gatccgcggt acgccgactt acaatgcgat gagagagttg accggaaaat
     2161 atcaaacagc caaaagcgac ggcggccatt tcattctcca cgaggtaagc cgttagaatg
     2221 ggggcatctc tgctttcaac ggcttttctt cttccgatac ttgaccgaag cctaggtttt
     2281 cgataagcgt tggcccggct cgtttccgag cctagcgtcg agctgatttc atcgacggcg
     2341 actttcggtt gcaggaggcg caacatgaag cacgcgttgc tggtgattct ttgctcgttc
     2401 ctgtggaccc atagcgctaa cgcccaagcc ccgttctatc aggggaaaac aattcgggtc
     2461 atcgtcggca cgccgccggg aaatctttat gacttctggg cgcgcctcat cgcagaacat
     2521 ctcggcaaac acataccggg caatccgatt tttgtcgtgc agaata
//
LOCUS       DASULZ010000376         4213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_248970, whole genome shotgun sequence.
ACCESSION   DASULZ010000376 DASULZ010000000
VERSION     DASULZ010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248970"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..544)
                     /locus_tag="VFY96_07795"
     CDS             complement(47..544)
                     /locus_tag="VFY96_07795"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5606400.1"
                     /translation="MKQEKAQLVIQGLKEAGVDFTAGVPDAQFIQVYKMLETDPEIRY
                     VGTANEAEAAGVGYGAWFGGKKPALILATSGLLVATYHLARINILHEVPLLVVIPYRG
                     DIGDPRWMGLYKKTTEPGLNAMDIPYRVVSRSSDIVRTIKECNESARAWLRCIAVILN
                     EEALW"
     gene            complement(567..1622)
                     /locus_tag="VFY96_07800"
     CDS             complement(567..1622)
                     /locus_tag="VFY96_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606401.1"
                     /translation="MKANRLLTVVSLAVLLSCFAYEGAFSQAPFYQGKTITIIRGSAP
                     GGVGEMRTRAVANYLKKHVPGNPTVLIEFMAGGGGTKAANHIYRGARADGLVMGSSPS
                     GMVSSAVLGEAGVQYDLDKFIYLGSPNSESHYVFFTNRKLGLGSMEKLRAHSGLRIGA
                     QTVGHPIYYTGRLFALILGLKDPKFVVGYSSPELDVALLSGELDATPGVASSVVKQNP
                     EFFDKGLVDFHSIIEIPRGDKHPRFAQLPELDGFVKSDKERKLLALHRTFRLAGSPFI
                     LPPATPKDKADILREAMRKIYTDKEFLAEYQKMTGEEASPLLPEANDKAIKELPRDPE
                     IIEFYKSLGGTQALPAR"
     gene            complement(1609..3891)
                     /locus_tag="VFY96_07805"
     CDS             complement(1609..3891)
                     /locus_tag="VFY96_07805"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5606402.1"
                     /translation="MTDRLVRSIERAVVSATTRDFSIIGRSIPRVDGIDKVTGRAKYT
                     GDLIVPGMLEGKFLRSPYAHARIRSIDTAEAEAMRGVIAVLTSEDLSDIETYIGRGKN
                     KDQPIVALDRVIFSGQPVAAVAAVDRSTAEEALSKIHVDYEELPAVISIEEAMAEGAP
                     RLHEFAERNLCAHHELVKGDVKKGFAEADVIVEDEFDFPMIYHYSMEPHVAIAQVDHE
                     GITIWTSTGHPFGVRAEVAQVFRVPLSRVRVHVNYVGGAYGSKSGGKIEPLVAALAQK
                     AQRPVRVVTSVADAMVTCRRHAIKCKVKTGAKKDGTLVAKQADIFLNTGAYAETGPIV
                     AGRTLTRILGPYRYPNLKINSYCIYTNTVSAASFRSIGGPQTAWATESQLDILAEKLS
                     IDPVQLRLKNLVAKGEELRPKYRPLDTDLAKDFKLVTDKLGWDGPVSKGGHGRGVGFG
                     TTDPGAPLASTSTVHVLADGSVVFMCGTSELGQGAKTIMSQIIAEELRVPLERVTIRP
                     IDTAFTPFDRSTGSSRSTTVMGKAVELAGSDARRQIVELAVEHFECPEEAISLREGEA
                     IAGGKKISYGELINKHFADQGGELVGTGYAHSGMAPTPANPLFWEIGIGAVEVSVDRE
                     TGKVHVEKYVTAADAGKAIHPLQCEGQDEGSAMMGFGHTFYEAYQWEGGQIINSSLID
                     YKVPTFGDVPDEFESILIEDGNGPGPYGAKGLGEGGIIPVAPAVANAIFWCTGARIKT
                     LPLTPEKVWRALKEVSSESK"
BASE COUNT          893 a         1264 c         1092 g          964 t
ORIGIN      
        1 tcttgcggca cgatcgaagc caattctttt atcaacgcca agcgagctac cataacgcct
       61 cctcattcag aatcaccgcg atgcacctga gccaggcccg ggcactctcg ttgcactcct
      121 tgatcgtgcg cacaatgtcg ctcgatcggc tgactacccg atagggaata tccattgcgt
      181 tcagtcccgg ctccgtagtt tttttgtaaa gtcccatcca gcgtggatcg ccgatatcgc
      241 cgcggtaggg aatcaccacc aatagcggca cctcgtgcag aatattgatg cgcgccaaat
      301 gataggtcgc gaccaacaat cccgacgtgg cgagaatcaa cgccggtttt tttccgccga
      361 accatgcgcc gtaacccact ccagccgctt ccgcttcgtt ggcggtcccg acataacgga
      421 tctcgggatc cgtttcgagc attttataaa cctgaataaa ttgggcgtcc ggcactccgg
      481 cggtaaaatc cactccggct tccttgagtc cttgaatgac caattgggcc ttttcttgct
      541 tcatatcgtt tcctctatgt tcaaccttaa cgcgccggca gcgcttgagt gccaccgagc
      601 gacttataga actcgatgat ttcaggatcg cgcggcaatt ccttaattgc cttatcattg
      661 gcctccggca aaagcggcga cgcttcttcg ccggtcatct tctgatactc ggcaagaaat
      721 tccttgtcgg tgtaaatttt gcgcatggcc tcgcggagga tatccgcttt atccttcgga
      781 gtagcgggcg gcaagatgaa cggtgagccg gcaagcctga aggtgcgatg cagcgccagc
      841 aacttgcgct ccttgtccga tttgacgaaa ccatccagtt ccggcagttg cgcgaagcga
      901 gggtgcttgt ctccgcgggg aatttcaatg atggaatgaa aatccactaa acccttatcg
      961 aaaaattcag ggttctgctt gacgacgctg ctggcgacac ccggggtggc gtccaactcg
     1021 ccgcttagca aagctacatc caactcaggt gaagaatagc cgacgacaaa cttcggatcc
     1081 tttaagccaa gaataagagc gaacaggcgg ccggtatagt agattggatg gcctacggtc
     1141 tgcgcgccaa tgcgtaaacc cgagtgagcg cgcagttttt ccatgctgcc caatccgagt
     1201 tttcgattcg tgaaaaatac atagtgcgac tcgctgttcg gcgagcccag ataaatgaat
     1261 ttgtcgagat cgtattgcac tcccgcttcg cccaaaacgg ccgacgacac cataccgctc
     1321 ggggaactcc ccatgaccaa tccgtcggcg cgcgcgccac gatagatatg gttagccgcc
     1381 ttggtgcctc cgcctcctgc catgaactcg atcaacacgg ttggatttcc cgggacatgc
     1441 tttttgagat aattcgccac cgccctcgtg cgcatctcgc ctacgccccc tggtgcgctg
     1501 ccgcgaatga tggtgatggt tttgccctga taaaaggggg cttgcgaaaa cgcgccttcg
     1561 taagcaaagc aactcagtaa aacagccagc gacacgactg ttagcaatct atttgctttc
     1621 actggagacc tccttgagag cccgccagac tttctccggt gtcagtggga gcgtttttat
     1681 gcgcgcgccg gtgcaccaaa agatcgcgtt cgccaccgcg ggcgccaccg gaatgatacc
     1741 gccttcgccc agccccttgg cgccgtacgg tccgggacca ttgccatctt ctatgagaat
     1801 cgattcgaat tcatcgggga catcgccgaa ggtgggcacc ttgtaatcga tcagggacga
     1861 attgatgatc tgcccgccct cccactgata cgcttcgtag aacgtgtgac caaagcccat
     1921 catcgccgag ccttcatcct gtccttcgca ctgaagcggg tggatcgcct tgccggcatc
     1981 cgcggcagtg acgtattttt cgacatgaac cttaccggtc tcacgatcga ccgaaacttc
     2041 cactgcgccg atgccgatct cccagaacag cggattcgcc ggcgttggtg ccatcccgct
     2101 atgcgcgtaa cccgtgccca ctagctcgcc gccctgatct gcgaaatgct tgttgatcaa
     2161 ttcgccgtag ctgatcttct taccgcccgc gatcgcttcg ccctcgcgaa gcgagatcgc
     2221 ctcctcagga cattcgaaat gttctactgc gagctcgacg atttggcggc gcgcatcgct
     2281 gcccgccaac tccaccgctt tgcccatgac cgtcgtcgaa cggctcgatc ccgtcgaacg
     2341 atcgaaggga gtgaatgccg tatcgatggg ccgaatcgtg acccgctcga gcggaacgcg
     2401 gagctcttcg gcgataatct ggctcatgat cgtcttggcg ccttgaccca gttcggaagt
     2461 gccgcacata aaaaccacgc tgccgtccgc gagcacgtgc accgtcgaag ttgacgccag
     2521 cggcgcaccc gggtcagtcg tgccgaaacc gactccgcgg ccgtgcccac ctttcgaaac
     2581 tggaccatcc caacccaatt tatccgtaac cagcttgaag tctttggcga gatcggtgtc
     2641 gagcggccgg tatttcggtc tcagctcttc accctttgcg accagatttt tgagccgtag
     2701 ctgtaccggg tcaatgctga gtttctccgc gagtatgtcc agctgcgatt ccgtcgccca
     2761 ggcggtttgc gggccgccga tggaacggaa ggaagcggct gaaaccgtat tagtgtaaat
     2821 gcaataagaa ttgatcttta gattcggata acggtacggg ccgagaatcc gcgtcaacgt
     2881 ccgtccggca acgatcgggc cggtttccgc gtaggcgcca gtgtttagaa agatatcagc
     2941 ctgcttcgcg actaatgttc catccttttt cgcgccggtt ttgactttgc atttgatcgc
     3001 atgccgacga caggtcacca tcgcgtcagc aaccgatgta accacgcgca caggccgctg
     3061 cgccttttgc gccaaggccg ccacgagcgg ctcaatctta cccccggact tgctcccgta
     3121 ggcgccgccg acataattca cgtgaacccg aactctggac agcggcacgc gaaacacttg
     3181 tgcgacctcg gcgcgcacgc cgaacggatg gccagttgaa gtccagatcg tgattccctc
     3241 atgatcgact tgcgcgatgg caacgtgcgg ttccatcgag tagtgataga tcatcggaaa
     3301 atcgaattcg tcttcgacga tgacgtccgc ctctgcgaaa ccttttttca catcaccctt
     3361 gacgagctcg tgatgcgcgc aaagattccg ttcggcgaat tcgtgcaagc gcggcgcgcc
     3421 ttcggccatt gcctcctcga tactgatgac cgccggcaat tcttcgtaat cgacatgaat
     3481 tttgcttaac gcttcttccg ccgtagaacg gtctacggca gccactgccg ccacgggttg
     3541 tccggaaaag atcacgcgat ccaacgcgac gatgggctga tctttgtttt tgccgcggcc
     3601 gatataagtt tcgatatcgc tgagatcctc gctcgtcagc accgctatga cacctcgcat
     3661 cgcttcagct tcggctgtgt cgatggagcg aatgcgcgcg tgagcgtaag ggctacgcaa
     3721 aaacttgcct tcgagcatcc caggaacgat tagatcgccg gtatactttg cccgaccggt
     3781 taccttatcg atgccgtcga cgcgcggaat tgatcggccg ataatagaaa aatctcgagt
     3841 tgttgcgctc actacagccc tttcgatact tcgaacaagg cggtcggtca tgcgttataa
     3901 acattcctcc cgtataacga gcatctgttg ctgtcaagat gaactgacgg tggcttgagc
     3961 gataacagtc ttgatgcgat cctctcttca atcgatggcg caagtaagaa caaacttttg
     4021 gacggcggtc atcgcggctg gcgcagtttt ctgttcgggc atggcatgct tccttccgga
     4081 catgcctgtg acttcaaaca actcatctct cgttgatatc agccccatcg atgtagacgc
     4141 gaagcatccc gaaagaaccg agttcggcgc cctgacgctc atgagcgctt ttcagttgtc
     4201 gtccagagac aaa
//
LOCUS       DASULZ010000377         3392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_554188, whole genome shotgun sequence.
ACCESSION   DASULZ010000377 DASULZ010000000
VERSION     DASULZ010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554188"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..639)
                     /locus_tag="VFY96_07810"
     CDS             complement(58..639)
                     /locus_tag="VFY96_07810"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5606403.1"
                     /translation="MNPKPGTHSEEHLDLVFLFALQALPANEIAAIEAQISSCEVCRQ
                     EMETLRPIVRSFVGWSTDVLRPAESLWGRLAKRIGSETAPRPFIPPLEATVKPEWEEP
                     TPGIHVKVLARNAENDSVSMLVRLDPGTDYPGHRHAGIEQLHLLDGVLKVDDRTLYPG
                     DFIHSEAGSVDRRVWSEAGCTCFLVTSTKDALL"
     gene            complement(636..1268)
                     /locus_tag="VFY96_07815"
     CDS             complement(636..1268)
                     /locus_tag="VFY96_07815"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5606404.1"
                     /translation="MIAIVAAPDKKTLGDILDVDKSKVRVSEDQWLQLVRAVAGGDQR
                     ALHSLYEQTYRIVFTLIVRITANQETAEEVTLDVFYDVWRKAGSYDPANGSVVGWIMN
                     QARSRAIDRLRFDQRRKRVNTYPDSLQPTTDIVDPQQAFLFEEQNRLLRNSLAVLTPE
                     ERNAIETAFFSELTYEETARKLNQPLGTVKTRIRSGMGKLRETLGRKLKG"
     gene            1644..1826
                     /locus_tag="VFY96_07820"
     CDS             1644..1826
                     /locus_tag="VFY96_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606405.1"
                     /translation="MSDETTTTTTSDLDLLIASMDDLARKIGEMEPDDPVRAVRLKEL
                     YTLTELSKSLKTRTGL"
     gene            complement(2308..2514)
                     /locus_tag="VFY96_07825"
     CDS             complement(2308..2514)
                     /locus_tag="VFY96_07825"
                     /inference="COORDINATES: protein motif:HMM:NF025156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEX5606406.1"
                     /translation="MQWEYKIVALGLIVDATEQNQREKHEQILNDLGREEWEAVAYIA
                     QSDTVLLKRPAQHPTDRKTTRQRR"
     gene            complement(2581..>3392)
                     /locus_tag="VFY96_07830"
     CDS             complement(2581..>3392)
                     /locus_tag="VFY96_07830"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554870.1"
                     /note="catalyzes the interconversion of alpha-D-glucose
                     1-phosphate to alpha-D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucomutase, alpha-D-glucose
                     phosphate-specific"
                     /protein_id="HEX5606407.1"
                     /translation="CSSPYAMAGLVQMKEKFQVAVGNDADADRHGIVTPTQGLMNPNH
                     YLAVAIHYLLNHRPNWKTAAVGKTLVSSSLIDKVVERLGRKLFEVPVGFKWFSPGLFD
                     GSICFGGEESAGASFLCRDGTVWTTDKDGIILSLLAAEIIATTGRDPGVWYEELANEF
                     GRPYYARMDLPATAPQKQALKSLRPETLSASELAGEPILAKLTKASGNGAEIGGLKVA
                     ARSGWFAVRPSGTEDLCKIYAESLRSEEHLKRIQDEALAIAQTVFNSQKVS"
BASE COUNT          779 a          963 c          862 g          788 t
ORIGIN      
        1 cctatttgcg aaacggtcag gctgtcttcg gctcgcgagc tggcaaattg accagaatca
       61 gagaagggcg tctttcgtcg aggtcaccaa aaagcaggta cagccggctt cactccacac
      121 acgacgatcg acacttcccg cctcactgtg aatgaaatca ccgggataga gtgttctgtc
      181 atcgaccttc aatacgccat cgagaagatg cagttgttct atgcccgcat gcctgtgtcc
      241 aggataatcc gttcccggat caagccgaac caacatgctg acgctgtcgt tttctgcgtt
      301 cctcgcgagt actttgacgt ggatacctgg agtgggttcc tcccactctg gtttaaccgt
      361 agcttccaac ggaggtatga acggccgcgg cgccgtttcg ctgccgattc gctttgcaag
      421 ccggccccat aaagattccg cggggcgcaa cacatcggtg gaccaaccga caaacgagcg
      481 cacaatcggc cggagcgttt ccatttcctg ccggcaaacc tcacaagagg agatttgagc
      541 ttcaatcgcc gcgatctcgt tcgcaggaag cgcctgcaat gcaaacagga acacgaggtc
      601 cagatgctcc tcactgtgtg tgcccggttt tgggttcatc cctttagttt ccttcccagg
      661 gtttctcgga gttttcccat tccggagcgg attcgcgtct ttacagttcc gagaggttga
      721 ttcaactttc ttgccgtttc ctcgtaagtt aattcagaga aaaaagcggt ttcgatcgca
      781 tttcgttctt cgggagtcag gaccgcaagg gaattccgca acaggcggtt ctgctcctca
      841 aacagaaagg cctgttgagg atcaacaatg tccgttgttg gctgcaggct gtccggatac
      901 gtgttcactc tttttcttct ctgatcgaac cgcagccgat caatcgccct gctgcgcgcc
      961 tgattcatga tccagccgac gaccgagccg ttagcaggat catagcttcc ggcttttcgc
     1021 catacgtcat agaacacatc gagcgtgact tcttcggcag tttcttgatt cgcggttatc
     1081 cgcacgatca acgtaaaaac gattcgatac gtctgctcgt aaagcgaatg caatgcacgc
     1141 tggtcaccgc cggcgacggc tcggacgagc tgtagccact gatcctcgga tacgcgaacc
     1201 tttgacttat cgacatcgag aatgtccccc agcgtcttct tatccggcgc agccacgatg
     1261 gcgatcatgc cgcgcgcact tctgcccgtc aagagaccgg aaaccgattc aggacttgtt
     1321 cctcgcatgc ggcgtgtagc gtttcgtcca cacgagaata aacgtcaaaa gtctgccacc
     1381 ggattgaaag aaaaaaatgc aatcgtgtcc cgacgccgca aaaaatcttg caaagtggaa
     1441 atccaagatc tgaattttga cgttattcaa gtgtccgttg ttggtgatca agtgcgaaaa
     1501 gttccccaag accaatccgt tacatagaaa gagcatgaat ttgggcattg agttctcgat
     1561 cgagtgccag ggcgaatgcc cgcacacgtt cgagacaatg caagttaaat gacccctatg
     1621 gcttatttcg gagggccgca tctatgtctg atgagacgac cacgaccacg actagcgatc
     1681 tcgatctgct gattgctagc atggatgatc tcgcccgaaa aattggcgaa atggaacccg
     1741 acgacccagt tcgcgctgtg cggctgaaag agctttatac gcttacagaa ctcagtaaat
     1801 cactcaaaac gcgaaccggg ctatgaagtt gcgctaccat tgccccctga ataaagatgg
     1861 tgccaaccgt tggccgccgc gctggcttca acccgataaa tctagccgag agttcgttcg
     1921 ccgtcctgca atcttcaaaa ttcgcgatgg aaacaatgag taaagtgcaa tcgcttcagg
     1981 acattatcgc gagatgatgg acatcgctat cccgcaggag cagaccagcc aaatggtcga
     2041 aacatcagca taggtgtgct atcctgatgc tatggcaaaa aaggaaacca agcggccgat
     2101 tgtaaagcgt accgagtgga gaattggcca gcccttggac tctccgcagg tccatagcgg
     2161 gccactacga gaaacggtaa acgaaccggt gcgcagccaa ttcggctcta agcggcaaaa
     2221 acgtaagaaa aagtagcaga ctttctctgt ctcttcccac cagtgaggaa cgctggcgta
     2281 tggcgcgtgg gcattcgccg ccgatgatca ccggcgttgc cgggtcgttt ttctatccgt
     2341 aggatgctgc gccggccttt ttaacagaac cgtatcgctt tgggcgatgt aagcgacggc
     2401 ttcccattcc tcccgcccga gatcgttcaa aatttgctca tgcttttcgc gttggttttg
     2461 ttcggtagca tcaacaatca agccgagcgc cactattttg tattcccatt gcatgtgttg
     2521 taatttttcc tagagcgcga ttgggtgtca aaccgccaaa ccaggcgctg agcgctcctc
     2581 ttaactgacc ttttggctgt taaaaacagt ttgcgcgatc gccagcgctt cgtcttgaat
     2641 gcgcttgaga tgttcttcgg agcgcaggct ttcagcgtaa atcttacaca aatcttccgt
     2701 ccccgaaggc cgcacggcga accagccgct ccgcgcggca acctttagac cgccgatctc
     2761 tgcaccatta ccggaagcct tagtcagctt tgcgagaatt ggctcacccg cgagctccga
     2821 tgccgacaag gtttccggac gcagcgattt cagcgcttgc ttctggggcg ctgtcgccgg
     2881 cagatccatg cgggcgtaat aaggccggcc aaactcgttt gccagctctt cataccaaac
     2941 tcccgggtcg cggccggtgg tggcgataat ttccgcggcg agcaaagaca agataatgcc
     3001 gtctttgtcc gtcgtccaaa ccgttccgtc gcggcaaaga aaactcgcgc cggcgctctc
     3061 ttcgccgccg aaacagatcg atccgtcgaa caaccccggc gaaaaccatt taaacccgac
     3121 cggaacttcg aacagttttc tgccgagccg ctcgacgacc ttgtcgatca gcgagctgct
     3181 caccagcgtt ttccccacgg cggcggtttt ccaattgggc cggtggttca acaagtagtg
     3241 aatcgccacc gcgaggtaat gattcgggtt catcaatcct tgagtcggtg tgacgatacc
     3301 gtgtcgatcg gcgtctgcgt cattgcccac cgccacctgg aacttttctt tcatctgaac
     3361 caatccggcc atggcgtacg gactcgaaca gt
//
LOCUS       DASULZ010000378         3579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_960338, whole genome shotgun sequence.
ACCESSION   DASULZ010000378 DASULZ010000000
VERSION     DASULZ010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960338"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VFY96_07835"
     CDS             complement(<1..452)
                     /locus_tag="VFY96_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX5606408.1"
                     /translation="MSVKSSKKPAPSRGAAASTQADTHKVLLLDGHSLAFRAFFALPD
                     TLVTSSGQVTNAVYGFTAMLIKLLADERPRGVLVCFDKGMPQFRIDRYAEYKAGRTAA
                     PDLFKQQLPLIREVVETLRIPIAELDGYEADDLLATITKHARQEGCEVV"
     gene            632..1504
                     /locus_tag="VFY96_07840"
     CDS             632..1504
                     /locus_tag="VFY96_07840"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX5606409.1"
                     /translation="MEYFEPRSVSEALSVLAKHGAEAKIIAGGTDVMVDIKFKEEPGG
                     LVNIKKIPGLSGISENGAGLRIGPLITIRDIETSTLVHNKLPVLWEAAHQFASLQVRN
                     TATIGGNICRASPSGETLAPLLVLEAKAKLAFSDGEKSVPFTSFFQGPGKSSAGSNGL
                     LTEIEIPYPPAGSKGVYLKHAVRGAMDIAMVGVAVLMTPDSGKNNIQDVRIGLGAVAP
                     TPVRAAKAEALLRGKTLTAPLLREAAALAASESSPIDDQRSSAEYRRWIVEALTRKGL
                     EQTWKAATGKEIAS"
     gene            1501..1965
                     /locus_tag="VFY96_07845"
     CDS             1501..1965
                     /locus_tag="VFY96_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5606410.1"
                     /translation="MSITITTKINGHSVTATADASTSLLEFLRDILEMKGSKLCCNTG
                     ECGACTVIYNGKPINSCVTMAADANDAEITTIEGIAEGDKLHPVQQAFIDTGAVQCGY
                     CTPGFIMSVKALFDRTKNPTTQDIEEAISGNICRCTGYNKIVDAIQLAAQKL"
     gene            2126..>3579
                     /locus_tag="VFY96_07850"
     CDS             2126..>3579
                     /locus_tag="VFY96_07850"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5606411.1"
                     /translation="MAKAEFKVIGSSPARIGGIERVIGKGVYGIDLMLKDQLHGAILR
                     SQYAHAKILSIDTSEAKKIPGVHAVVTADDAPNVRYGRSYIDRHILARGKVRFMGDPV
                     AAVAAQSPAIARQAVKKIKVVYEPLPVVIDPEEATKPHAPVLHEDMPLPKTIPADAGL
                     KNICGHAVVKVGDAEKAMAEADVVVEEAYETKMIHPQYLEPRIAAAQMEPNGRLTVWA
                     NAQAPFPVRQEVAKTLGLPLNNVRVISAGLGGGFGGKGSGITSGAAIEPICGLLAIKA
                     NRPVMIVLDKAEETISTTIRAGAKMYIKTGVKKDGTIIARTGKVFYDAGGYSGFGVQA
                     GGRCTNMLGGWYLMPNCHIEGYTVYTNKQVCGPVRGPGGPQAAFAVESHMDSIAAKLG
                     MDPVEFRLKNIPKPGDKIVGVPKLRDVSLGETIRIAKEKIGWGNVKLQKNQGIGIATG
                     SWIESAGPGGGAIVKVNEDGSVTVHIGKIDMGTIP"
BASE COUNT          857 a         1015 c         1039 g          668 t
ORIGIN      
        1 accacctcac aaccctcttg tctcgcgtgt ttggtgatgg ttgccagcaa atcgtccgct
       61 tcatagccgt cgagctcggc aatgggaatg cgcaaggtct cgacgacttc gcggatcagc
      121 ggcagttgct gtttgaacaa gtctggagcc gccgttcgtc cggctttgta ttccgcatac
      181 cggtcgatgc gaaattgcgg catccctttg tcgaaacaga ccagtacgcc ccgcggccgt
      241 tcgtcagcca agagcttgat cagcatcgcc gtaaaaccgt acaccgcgtt ggtgacttgt
      301 cccgaagacg tcaccaacgt atcgggcaac gcgaaaaacg cgcggaacgc caacgaatga
      361 ccgtcgagca acagcacctt gtgtgtatcg gcctgcgttg atgcggccgc gccgcgcgac
      421 ggcgcaggct ttttacttga tttgacgctc acagcccgca gtctaacggt tggtccaaac
      481 gacggcaact gttacggact tgcgcagatc gacccgaaaa caacttggga agtcaaaaag
      541 caaaactctc tgtcctcttg ccatgctccg cggaattggg ctaagaggaa gagcaaattc
      601 accagaaaga attagtacag aggcagagcc tatggagtat ttcgaacctc gatcggtcag
      661 tgaagctctt tccgtgttgg ccaaacatgg cgcagaagcc aagatcatcg ccggcggcac
      721 agatgtcatg gtcgatatta agttcaagga agaacctggc ggtttggtca acatcaaaaa
      781 gattccgggc ttgagcggga tcagcgaaaa tggcgccggt cttcgcatcg gtcctttgat
      841 cacgatccgc gacattgaaa ccagcactct cgtgcataac aagctgccag tgctctggga
      901 agcagcgcac cagttcgcgt cattgcaagt gcgcaatacc gccaccattg gcggaaatat
      961 ttgccgggcg tcaccctcgg gtgaaacctt ggcgcctttg ctggtgctcg aggcgaaagc
     1021 gaaattagcc tttagcgacg gcgaaaaaag cgttccgttc acgtcgtttt ttcaaggccc
     1081 cggcaaaagc agcgcgggta gcaacggctt gctgacggaa atcgaaattc cttatccgcc
     1141 tgcgggcagc aaaggcgttt atctcaagca tgccgtgcgc ggcgcgatgg atatcgctat
     1201 ggtcggcgtc gccgtgctca tgacgccgga cagcggtaaa aataatattc aagatgtgcg
     1261 catcggtctc ggcgccgtag caccgacgcc ggtcagagca gcgaaagccg aagcgttgct
     1321 ccgcggcaaa acgctgactg ctcccctgct cagggaagct gcggcgctgg cagcctcgga
     1381 atcaagcccg atcgacgatc aaagaagcag cgctgaatac cgtcgctgga ttgtcgaggc
     1441 gcttacgcgc aagggcttgg agcagacttg gaaagccgcc accggcaagg agatcgcgtc
     1501 atgagtatta cgattactac gaaaatcaac ggccattccg tcaccgcgac ggctgatgct
     1561 tcaacctcac tgttggaatt cctgcgagac attttggaga tgaagggcag caagctgtgc
     1621 tgcaacaccg gcgagtgcgg tgcctgcacg gtcatttaca acggtaagcc catcaactcg
     1681 tgcgtgacca tggccgcgga tgccaatgat gcggaaatca cgacgatcga agggatcgcc
     1741 gagggcgata aattgcaccc cgtgcagcag gcgttcatcg acacgggcgc ggtgcagtgc
     1801 ggctactgca cgccgggatt cataatgtcg gtgaaagcat tgttcgaccg aaccaaaaac
     1861 ccgacaacgc aagatatcga ggaagctatt tcgggtaata tctgccgctg caccggttac
     1921 aacaagatcg tcgacgcgat ccaactcgca gcgcagaagc tttagcttta agagtgaatc
     1981 gggaacagat acagccaaca aaggtgccaa gacgctaagg atcgtttgtc atttcgaaca
     2041 aagagagaaa tctttcgtag atcgccctct cttcggatga caggtcaccg cccctggtgc
     2101 atttcctaat aaggagacca tcttcatggc aaaagctgaa ttcaaagtga ttggttcgag
     2161 cccggcccgg atcggcggca tcgagcgggt tatcggcaag ggtgtctacg gcatcgacct
     2221 gatgctgaaa gaccaactcc atggcgcgat tctgcgcagc cagtacgccc acgcgaagat
     2281 cttgagcatc gacaccagcg aagcaaagaa aattccgggc gtgcacgctg ttgtgaccgc
     2341 ggatgacgca cccaacgtgc gctacggccg cagctatatc gatcgccata tccttgcgcg
     2401 cggcaaagtg agattcatgg gcgacccggt agccgccgtg gccgcacaat cgccggccat
     2461 cgccaggcag gcggttaaaa aaatcaaagt cgtctacgag ccgttgccgg tggtgatcga
     2521 ccctgaagaa gcgaccaaac cacacgcccc ggttctgcac gaggacatgc cgctgccgaa
     2581 aacgattccc gcggacgcag ggctcaaaaa tatttgtggt cacgccgtcg tgaaagtcgg
     2641 cgatgccgaa aaggcgatgg ccgaagcgga tgtggtcgtc gaggaagctt acgaaaccaa
     2701 gatgatacat ccgcagtatc tggagccgcg catcgctgcc gcgcagatgg agcccaacgg
     2761 tcggctcaca gtttgggcca atgcccaagc gccttttcca gttcgccagg aagtcgcgaa
     2821 gaccctcggc ctgccgctga acaacgtgcg cgtgatctcc gccggcttag gcggcggctt
     2881 cggcggcaaa ggcagcggca tcacctctgg tgccgcgatc gagccgatct gcggactctt
     2941 ggcgatcaag gcgaaccggc cggtgatgat cgtcctcgac aaagcggaag aaacgatttc
     3001 gacaaccata cgcgccggcg ccaagatgta catcaagacc ggggtaaaga aagacggcac
     3061 catcatcgcg cgcaccggaa aagtatttta tgacgccggc ggctactcgg gcttcggcgt
     3121 gcaggccggc ggccgctgca ccaacatgct cggcggctgg tatctgatgc ccaactgtca
     3181 catcgagggc tacacggtct acaccaacaa gcaggtgtgc ggtccggtac gcggacccgg
     3241 cggtccgcag gccgcttttg cggtggaatc gcacatggac tccatcgctg ccaagcttgg
     3301 aatggatccg gtggaattcc gcctgaagaa cattcccaag cccggcgaca aaatcgtcgg
     3361 cgtgccaaag ctgcgcgatg tttctctggg cgagacaatt aggatcgcca aggagaaaat
     3421 cggctggggc aacgtgaaac tgcaaaagaa tcagggcatc ggcatcgcca ccggttcatg
     3481 gatcgagagc gccggtcccg gcggtggtgc aatcgtcaag gtcaacgaag acggctctgt
     3541 cacggtgcac atcggtaaga tcgacatggg caccatccc
//
LOCUS       DASULZ010000379         3377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_960369, whole genome shotgun sequence.
ACCESSION   DASULZ010000379 DASULZ010000000
VERSION     DASULZ010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960369"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VFY96_07855"
     CDS             <1..483
                     /locus_tag="VFY96_07855"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX5606412.1"
                     /translation="GTLGLTQLFQVERENHCLEALLLSPLDRGALFLAKSCFNLLLML
                     LVELVVMPLFWILFNLHGWNLLPRLFLVTLLGTVGFCVLGTIMSAITLRARAREILLP
                     LVLFPLMIPVILATIRCMENIMSAGDLGAAGSWLRLLLGFDVIFVTVGILIFDRVIEG
                     "
     gene            488..1192
                     /gene="ccsA"
                     /locus_tag="VFY96_07860"
     CDS             488..1192
                     /gene="ccsA"
                     /locus_tag="VFY96_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX5606413.1"
                     /translation="MPAAQPVRKRRGILGALSLVAIGIGLYWGLVIAPPDAYQGEVQR
                     IMYLHLPSILTAYLSYFLVFIGSCMYLWKREKRDDILAHSAAEVGVLFTGLTIIEGSI
                     WGRPTWGVWWTWDARLTLTAILLLIYTGYLMLRSLIEDDHRAASAAAVLGIIGFLDIP
                     LIHMSVYWWRTLHQPPSILQPNKAPWENIAWPLLIPLLINILAFLLLLFYLLSLRYHL
                     GEMRDEIRMRRLEQSA"
     gene            1192..1347
                     /locus_tag="VFY96_07865"
     CDS             1192..1347
                     /locus_tag="VFY96_07865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HEX5606414.1"
                     /translation="MDHWSFVALAYGIVWSVIVVYFFSLKRRYRKAQMELSALTSAQT
                     LKEHEQT"
     gene            1334..1777
                     /gene="ccmE"
                     /locus_tag="VFY96_07870"
     CDS             1334..1777
                     /gene="ccmE"
                     /locus_tag="VFY96_07870"
                     /inference="COORDINATES: protein motif:HMM:NF009727.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX5606415.1"
                     /translation="MNKRKRFLIGGCVILAAISYLIVGGMKEAMVYFKMPSELRAEES
                     SMQGKFVRMGGMVVNGSLHKDLQNLTYRFELTDGNGTFPVYFKGVPPDLFTEGKGAVV
                     EGRIGSDGIFHATTIMAKHAEEYSPHSEAQPATNSFIPAKETVSR"
     gene            1774..>3377
                     /locus_tag="VFY96_07875"
     CDS             1774..>3377
                     /locus_tag="VFY96_07875"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEX5606416.1"
                     /translation="MTAALGHTSILLAFIAALGGIASPIVAARSGDTRYLTVARLAIL
                     AQFVLVTLAATALIYGLVTTDFSIKYVAFNTTRATPVYYRITGLWGALEGSLLLWEWV
                     LIIFSGVVAWLYRDRHKDTLPWVIMIFCIVSAFFLGVVGFLSNPFETISPIPPDGRGL
                     NPLLEDANMMTHPPLLYTGFVGLTVPYAFAMAALIVGKLDEAWIVSTRRWTITAWFFL
                     TAGNLVGAWWSYHVLGWGGYWAWDPVENAAFMPWLPATAFLHSIQVQERRRMLKVWNL
                     SLIIVAFSLTIFGTFLTRSGILSSIHAFSSGPVGGFFLGFLAVILLGSFGLVAYRADL
                     LKEQPELDSMVSRESAFLLNNIVLVSALFTIFLGTIFPLISEAVAGVQVSVGAPYFNS
                     VTVPLFLFLVFLMAVGPIIAWRRASWDNLKRNFTWPAAAALLLGFGLFAWKVRDFMPL
                     LGFTLLAFVAFSILYDTALALRARRRIAGEGVIRGLVTLARRNQRRYGGFIVHLGIVL
                     IMMGIAGSMSYSVEKEATMSLNQQLQV"
BASE COUNT          595 a          921 c          963 g          898 t
ORIGIN      
        1 ggcacactcg ggctgacgca gttatttcag gtggaacgcg agaaccattg cctcgaggcg
       61 ctccttttat cgccgcttga cagaggcgcc ctttttctcg ccaagagttg ttttaatctg
      121 cttctgatgt tgctggtaga acttgtcgtg atgccgcttt tctggattct gttcaatttg
      181 cacggatgga atttgctacc gcgactattt ctagttacct tattaggcac cgtcggcttt
      241 tgtgttttgg gaaccatcat gtcggccatc acgctccggg cgcgggcgcg cgagattttg
      301 ttgccgctgg tgcttttccc actgatgatt cccgtgatcc tggcgacgat tcgttgcatg
      361 gaaaacatta tgagcgcggg cgatctcggc gccgcggggt catggctgcg gctgcttctc
      421 ggttttgacg tgatattcgt gacggtgggc atattgattt ttgatcgagt cattgaaggt
      481 tgacgccgtg ccagcggctc aaccggtaag aaaacgcaga gggattcttg gcgctttgtc
      541 gctggtcgcc atcggcatcg gcctctactg ggggctggtg attgcgccgc cggacgccta
      601 tcaaggcgag gttcagcgca tcatgtacct gcacctgccg agtattttga ccgcctatct
      661 atcctatttt ctcgttttta tcggcagctg tatgtatttg tggaaaaggg aaaaacgtga
      721 cgatatcctc gcccattcgg cggcagaagt cggtgtgctg tttacggggc tgacgattat
      781 cgaaggctcg atctgggggc ggccgacctg gggtgtatgg tggacctggg atgcccgcct
      841 gacgctcacc gcgattctgt tgttgatcta taccggttat ttgatgctgc gctcgctcat
      901 cgaggatgat catcgggccg cgtcggcggc cgccgtgctg ggcatcatcg gatttctcga
      961 cattccactg atccatatgt cggtctactg gtggcgcaca ttgcatcaac cgccatcgat
     1021 ccttcagccc aacaaagcac cctgggaaaa tattgcctgg ccgcttttga ttcctctact
     1081 gatcaatatc ctcgccttct tgcttctgct gttttatttg ttgtcgttgc gctaccactt
     1141 gggcgaaatg cgcgacgaaa tcagaatgcg gcgtctggag caaagcgcct gatggaccac
     1201 tggagtttcg ttgcgctggc gtacgggatt gtctggagtg tcatcgtggt gtactttttt
     1261 tctctgaaga ggcgctacag aaaagcgcag atggagttga gcgccctcac atcggcacag
     1321 acgctcaagg aacatgaaca aacgtaagcg atttttgatc ggcggatgcg tgattctggc
     1381 cgcgatctcg tatttaatcg tcggcggcat gaaggaagcg atggtctatt tcaagatgcc
     1441 gtcagaattg cgcgccgaag aaagctccat gcaaggcaag ttcgtgcgca tgggcggtat
     1501 ggtcgtcaac ggctcgctgc acaaagatct gcaaaatctg acttaccggt tcgagctcac
     1561 cgacggcaac ggcacatttc ccgtctactt caaaggcgtg ccgccggatt tgtttaccga
     1621 gggaaaaggc gcggttgtcg aaggacggat cggcagcgac ggcatctttc atgccacgac
     1681 aatcatggcc aaacacgcgg aagagtacag cccccacagc gaagcgcagc cagccaccaa
     1741 tagttttatt cccgccaagg aaaccgtctc ccggtgaccg ccgctttagg tcatacatcc
     1801 attctgctgg cgttcattgc ggcgctcggc ggcatcgcaa gccctatcgt cgcggcgcgt
     1861 tccggagaca cgcgctattt gacagtggca cggctggcga ttctcgcgca gttcgttttg
     1921 gtgaccctgg cggctacagc tctcatttat gggcttgtga caaccgactt ctcgataaaa
     1981 tacgtcgcct tcaacaccac ccgggcgacg ccggtttact atcgaatcac tggtttgtgg
     2041 ggcgcgctgg aaggatcgct gctgctctgg gagtgggtgc tgatcatctt ttccggcgtg
     2101 gtggcatggc tctatcgcga tcgtcacaag gatacgttgc catgggtcat tatgattttc
     2161 tgcatcgttt cagcgttttt tctcggcgtc gtcggcttct tatcgaatcc cttcgaaacc
     2221 atttctccca tcccgcccga cggccgcggc ttgaacccgc tgctcgagga cgccaacatg
     2281 atgactcacc caccgctgct ttataccggt ttcgtcggtc tcaccgtgcc gtatgccttc
     2341 gccatggcag cactgatcgt cggcaaactc gacgaagcct ggatcgtcag caccagacgc
     2401 tggacgatta ccgcgtggtt ctttttaacg gcgggcaatc tggtcggcgc gtggtggtcg
     2461 tatcatgttc tcggctgggg cggttactgg gcgtgggatc cagtggaaaa tgctgcgttc
     2521 atgccgtggc tgccggcaac ggcctttttg cactcgattc aggtccagga acgccggcgc
     2581 atgctgaagg tttggaactt atctttgatc atcgtggcct tcagtctgac gatcttcggg
     2641 acgtttctta cccgctccgg aattctttcc tcgattcacg cgttttcgtc cggaccggta
     2701 ggcggatttt ttctcggctt tctcgccgtc atcctgctcg gctccttcgg cctggtcgcc
     2761 tatcgcgctg atctgctcaa agagcagccc gagctcgatt ccatggtcag ccgcgaatcg
     2821 gcttttttgc tcaacaatat tgtcctggtg tcggcgctct tcacaatttt ccttgggacg
     2881 atttttccgc tgatctccga agctgtagca ggtgttcaag tgagtgtggg agcgccgtat
     2941 ttcaactccg tgaccgtgcc actctttttg tttctggttt ttttgatggc cgtcggtccg
     3001 attatcgcgt ggcggcgcgc ttcttgggac aatctaaagc gcaacttcac ctggccggcg
     3061 gcagcggcgt tgctgctcgg tttcggtttg ttcgcgtgga aagtccgcga ctttatgccg
     3121 ctgctgggtt ttacgctcct ggccttcgtc gcgttcagca tcttgtatga cacggcactg
     3181 gcgttgcgtg cgcggcggcg catcgccggc gaaggggtca ttcgcggttt ggtcacactg
     3241 gcgcgccgca atcaacgccg ctatggaggc tttattgttc acctcggtat cgtgctgatt
     3301 atgatgggca tcgccggctc gatgtcctac agcgtcgaaa aggaagcgac catgtcgctc
     3361 aatcagcagc tccaggt
//
LOCUS       DASULZ010000380         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_555067, whole genome shotgun sequence.
ACCESSION   DASULZ010000380 DASULZ010000000
VERSION     DASULZ010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555067"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="VFY96_07880"
     CDS             complement(<1..1036)
                     /locus_tag="VFY96_07880"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEX5606417.1"
                     /translation="MKSPRKLFVAVAVVIIIAGFGLARTIDYILAQNRDQVRQELQKI
                     IGKDVSFAGLAAVWWGRPGFVVREFRILDDSHFAATPLVSAKELLLGISLWNLLFQRL
                     VITQLTFIAPELQIIINETGALNLASLLERKNELREFPTLQYPVTPERKQAGVNFSIA
                     RIDIKDGRVEYIDRSIKEPAELRMNHLRLTLKGFESRDAVEIRLAASLTEGLNQDLRI
                     DGRLDPAPDGMPWNRREAHLNLQFDSLYPPVVGHAIAGLRNKIPSELIVTGPMALKAT
                     ARGTPERPRIEDIDLKIPLLGSSDYNAMLTGRVEFSERRSWDNAEIHGKLVVKPLAVT
                     ALRSIRWLETL"
     gene            complement(1188..>2267)
                     /locus_tag="VFY96_07885"
     CDS             complement(1188..>2267)
                     /locus_tag="VFY96_07885"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX5606418.1"
                     /translation="GIHKEEIEDVYFGCTNQAGEDSRNVARNASLLAGLPYTIPGATV
                     NRLCGSGLEAVNQAGRAVETDHGDLFIAGGVESMTRGPWVMGKPTAAFQRGDVQMYDS
                     SLGWRFPNKRMAELYSLINNGETAENVADKYQISREDQDKLALASHKKALKAQQDGRL
                     KDEIVAVSIPQRKGAPVVHDKDEGPRADTSLEILAGLSPSFKKGGTVTAGNSSPLSDG
                     AAALLVTTPQKAEQLKLKPMARIIASATAGVHPNYMGIGPIPATEKVLKRAGLSIDQI
                     DLVELNEAFASQVLACARELGIDLDKLNVNGGAIALGHPLGCSGARLVTTLLHEMRKR
                     GSRYGLATMCIGVGQGIATVFERIH"
BASE COUNT          474 a          663 c          629 g          501 t
ORIGIN      
        1 gcagcgtttc tagccacctg attgaccgca aagccgtcac cgccaatggt ttcaccacca
       61 gtttgccatg aatttccgcg ttgtcccaag agcgtcgctc actgaactcc actctgccgg
      121 tcaacatcgc gttgtaatcc gaggaaccga gcagcggaat tttcaaatcg atatcttcga
      181 tgcgcggtcg ctccggtgta cctcgagcag tcgccttcaa tgccatcggc ccggtgacga
      241 tgagctcgct cggaatttta ttgcgcaagc cggcaatcgc atggcctacc accggcggat
      301 agagcgaatc aaactgaagg ttcagatggg cttcacgacg gttccacggc atcccatcgg
      361 gggcgggatc gagccggccg tcgatgcgca agtcttggtt aagcccctcg gtcaacgaag
      421 ccgcgagccg aatttccact gcatcacgtg actcgaagcc ctttaaggtc aggcgcaaat
      481 gattcatgcg aagttctgcg ggctctttga tcgatcgatc gatatactca acccgcccat
      541 ctttaatgtc gatccgagcg attgagaaat tcacaccggc ctgcttgcgc tcaggcgtga
      601 cgggatactg cagcgtggga aattcgcgga gctcattttt gcgttcgagc aagctggcga
      661 gattcagtgc gccggtttca ttgatgatga tctgcaattc cggagcgata aacgtgagct
      721 gggtgatgac cagccgctgg aataacaaat tccataaact gatgccaagc aggagctcct
      781 tggcgctgac gagcggtgtt gcggcgaaat gggaatcatc gagaatacgg aattctctca
      841 caacaaaacc gggccgaccc caccaaaccg ccgccagacc ggcaaagctg acgtccttgc
      901 cgattatttt ctgcaactcc tggcgtacct gatcgcgatt ttgagcaaga atgtagtcga
      961 tcgttcgtgc caatccgaag cctgcgatga tgatcaccac agcgaccgcg acaaagagtt
     1021 ttcggggact tttcattctg ccggcgctcg cgcaactggc gcggttcgtt gccggaaaga
     1081 gataaggagc cgatcagacc agtgcagccg cggcccgata caaaggcggc gttcaactag
     1141 accgatgaga ggttttttag cgatgcgtag aacgcgcagt ccggtattca gtgaatacgc
     1201 tcaaaaaccg tcgcaatccc ctggccgaca ccaatgcaca tggttgccaa gccataacgg
     1261 ctgccccgct tgcgcatttc atggagcaaa gttgtcacaa gccgtgcgcc actgcagccg
     1321 agtggatgac cgagcgcaat cgcgccgcca ttgacgttca gtttgtcgag atcgataccc
     1381 agttcgcgcg cgcaagccag cacctgcgat gcgaacgcct cgttcaattc caccagatcg
     1441 atctggtcga tgctcaagcc tgcgcgtttg aggactttct cggtcgcggg aatgggtccg
     1501 atccccatgt agttcggatg cacaccggca gtggccgaag cgattattcg cgccatgggt
     1561 tttaacttca attgttcagc cttttgtggc gtggtcacca gtaaggccgc ggcgccgtcc
     1621 gacagcggag aggaattgcc ggcagttacc gtgccacctt ttttgaacga cggcgacagc
     1681 cccgcgagaa tttccagaga agtatccgcc cgcggtcctt catctttgtc atgcaccacc
     1741 ggcgcgcctt tgcgctgcgg aatcgacacc gccacaattt cgtctttaag tcggccgtct
     1801 tgctgtgctt tgagcgcttt tttatggctc gcgagcgcca gcttgtcctg gtcttcgcgg
     1861 ctgatttgat atttgtccgc gacattttcc gctgtctcac cgttattgat cagcgaataa
     1921 agctcggcca tgcgcttgtt cgggaagcgc cagccgagcg agctatcgta catctgcaca
     1981 tcgccgcgtt gaaacgcagc agtcggtttg cccatgaccc acggtccgcg agtcatgctt
     2041 tcaacaccgc cggcgataaa caaatcgccg tgatcggttt cgaccgcgcg cccggcttgg
     2101 ttcaccgctt cgagccccga gccgcacagg cggttcaccg tagcgccggg gatggtatag
     2161 gggaggccgg cgagcaggga ggcgttccgg gccacattgc gcgagtcttc acctgcctgg
     2221 ttggtgcagc cgaagtaaac gtcttcgatc tcttccttat ggatgcc
//
LOCUS       DASULZ010000381         2337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_757341, whole genome shotgun sequence.
ACCESSION   DASULZ010000381 DASULZ010000000
VERSION     DASULZ010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757341"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /gene="rlmD"
                     /locus_tag="VFY96_07890"
     CDS             <1..954
                     /gene="rlmD"
                     /locus_tag="VFY96_07890"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEX5606419.1"
                     /translation="DFELKPIVPAPQEYYYRRRIRLQVGTNNVLGFFGTASHQLVEIR
                     SCLIADERLNNALEVLRDWIRRLRTAIEYVEIIAGDEPAEVVAVIGAAAPFASVDDSI
                     CQGLTRETETIRGLIVSDENWRRTWGQTAITIGLGAGLSLTVAADVFTQVNAAANRQM
                     LQALLTAAQFNRSDRVLELYCGAGNFTLSIAQEAQEVIGVDGDRLAIRHGKLNAQKND
                     LTNIRWTCAPVPKAVAEFRRRREEFTKTVLDPPRAGAKEIVASLPDLNASRIVYVSCN
                     PTTLARDLAELAKRGYRLQMVQPCDFFPQTFHVETLAVLDR"
     gene            complement(990..1919)
                     /locus_tag="VFY96_07895"
     CDS             complement(990..1919)
                     /locus_tag="VFY96_07895"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5606420.1"
                     /translation="MIIDTHFHAFPGKFLDLIPEARNDVRGVGFHAFDHREYLDVMDR
                     YGIDIGVLSNTGGRIEKSGDRQKALKLCQILNDAFAEAHAKYPKRFMAFARLPMIDME
                     DCTRELERCYKELKMHGVMMPTNVAGKYIDEPEFKPFWDALAQGDKPLFIQPANAPCH
                     SNWDKYSLHQKILWPTDSTLALSRIVYAGIFDRYPQLKMIASHLGGMILLYLDRLNWR
                     EGDPVCKEEPEAYFKKIYYDTAGPIRATFIKLVYDTVGADQILFGADYPHGRGGRDDQ
                     FYPMTLKAMMELEIPQSDKDKIYSLNAKKLFKL"
     gene            2029..>2337
                     /locus_tag="VFY96_07900"
     CDS             2029..>2337
                     /locus_tag="VFY96_07900"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX5606421.1"
                     /translation="MAEVSPTTRVPILIAGAGPVGLSLAIELAYRGIRCLIVERSDGS
                     VDFPTTNLANTRTCEHLRRWGIADHMRYQSGYPTDYPRNYLFVTRMTGYEIARFNHPA
                     N"
BASE COUNT          516 a          644 c          631 g          546 t
ORIGIN      
        1 gatttcgagc tgaagccgat cgtgccagcg ccccaggaat attattatcg gcgcagaatt
       61 cgacttcagg tggggaccaa caacgtgctc gggttttttg gcacagcttc ccatcaattg
      121 gtggaaatcc gctcatgtct gatcgccgac gaacgcctaa acaatgccct cgaagttttg
      181 cgcgactgga tccgccggct gcgcaccgca attgaatacg tcgagatcat cgcaggcgat
      241 gagcctgccg aagtcgttgc cgtgatcggc gccgccgctc cgttcgcgtc agtcgatgac
      301 tccatttgcc agggtctcac acgggagacc gaaacgattc gcggcttgat agtctctgat
      361 gagaactggc gccgcacatg gggccaaacg gcaattacga tcggcctcgg cgctgggttg
      421 agccttacgg tcgctgccga tgttttcact caggtgaatg cggcagcaaa tcggcagatg
      481 ctgcaggcgc tgctgaccgc ggcacaattc aatcgatctg atcgggtgtt ggagctttac
      541 tgcggcgccg gcaactttac gctttcaatc gcccaagagg ctcaagaagt gattggcgtc
      601 gatggcgatc gattggcaat acggcatggc aagctcaacg cacagaaaaa cgatttaaca
      661 aacattcgtt ggacttgcgc gccggttcca aaggcagtgg cggagtttcg gcgccggcgc
      721 gaagaattca ccaaaacagt actcgatcca ccgcgcgcgg gagcaaagga gattgtggcg
      781 agcttgcctg acctcaacgc aagccgcatt gtctatgtct catgtaatcc gacgactttg
      841 gcgcgcgatt tagccgagct cgccaaacgg ggttacaggc tgcagatggt acagccctgc
      901 gatttttttc cgcaaacgtt tcatgtcgag acgctcgccg tcctcgaccg ttagtgcgtc
      961 ttttcgacgg ttgaaacgca ttaaatgatc tatagcttga atagtttttt tgcgttcaac
     1021 gagtagatct tgtctttgtc cgactgcggg atttcaagct ccatcatcgc tttcagtgtc
     1081 atcggataaa attgatcgtc ccgcccgccc cggccatgcg ggtaatccgc gccaaacaga
     1141 atctgatctg cgcccaccgt gtcataaacg agctttatga acgtagcacg aatcggtccg
     1201 gcggtatcgt agtaaatttt tttgaaataa gcttcgggtt cttccttaca gaccggatcg
     1261 ccttcgcgcc agttgagccg gtcgaggtaa aggagaatca tgccgcccaa atgcgacgcg
     1321 atcattttaa gctgcggata acggtcgaag atacccgcat agacgatgcg ggacagcgcc
     1381 agtgtgctat ccgtcggcca aagaattttc tgatgcagcg agtatttgtc ccagtttgag
     1441 tgacagggcg cgttggccgg ctgaatgaac agcggtttgt cgccttgtgc aagcgcatcc
     1501 cagaagggct tgaactcggg ttcatcaata tatttccctg cgacattggt cggcatcatt
     1561 acgccgtgca ttttcagctc tttgtaacac cgctccaact cgcgcgtgca gtcttccatg
     1621 tcgatcatcg gcagacgagc gaaggccata aagcgcttgg gatacttcgc gtgcgcctca
     1681 gcgaaggcgt cgttgagaat ctggcatagc tttagcgctt tctgccgatc gccggatttt
     1741 tcgatcctgc cgccggtgtt cgaaagcaca ccgatatcga tgccatacct gtccatcacg
     1801 tcaagatact ctcgatgatc gaaagcgtga aaaccaacgc cgcgcacatc gttccgcgcc
     1861 tcagggatga ggtcgagaaa tttgccggga aacgcgtgaa aatgagtatc gatgatcatg
     1921 acggctcctt aacgaaggtt agattgaaat atgccaacgc aaatggctcg tcaagctacc
     1981 ctttcagcca tttgacagct ttgttcaccg tgtggttatt gcttgtcgat ggccgaggtc
     2041 agcccaacaa ctcgcgttcc cattctgatc gccggcgccg gtccggtggg actctcgttg
     2101 gcgatcgagc tcgcctatcg cggcatccga tgcctgatcg ttgaacgaag cgacgggagt
     2161 gtggattttc ccaccaccaa cctcgccaat acgcgcacct gcgagcatct gcgccgctgg
     2221 ggtatcgccg atcatatgcg ttatcaatcc ggatatccga ccgactaccc gagaaattat
     2281 ctgttcgtca cacgcatgac gggttacgag atcgcccgct tcaatcatcc ggcgaac
//
LOCUS       DASULZ010000382         3274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_656341, whole genome shotgun sequence.
ACCESSION   DASULZ010000382 DASULZ010000000
VERSION     DASULZ010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656341"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VFY96_07905"
     CDS             <1..501
                     /locus_tag="VFY96_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606422.1"
                     /translation="VTGFTGPEIYIALQRGEIDAHATGAARFVIEQPEWIEKKLVHLH
                     ATYTIPKGRHHPRFTHLPDYDSFAKSDTERKLLDMFRAFQYPRWPYIFPPGAPRDRVQ
                     IIREAMRKAFADPDFPGEFRRLMGDDPAPMAGEELERAIKELPRDKEVVQLYQKLAGA
                     DPLPQR"
     gene            767..1378
                     /locus_tag="VFY96_07910"
     CDS             767..1378
                     /locus_tag="VFY96_07910"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEX5606423.1"
                     /translation="MANTTERPLADALTAIKTRRSVKEYLPTEVPREWIEELLDAAHW
                     APNHKLTHPWRFHVFTGEGRERLVAARQAAVRWAAEKKGEPVSEDALNFARAKCFSSP
                     VVIIVSMVGDDKKIVDQENYAACWCAIENLLLAATARGLGSYPSTGDWIDQNFVGPVL
                     GLTEKERPVACIFLGYSEQKTMAKRLPVERHTTWYTEAGTNSQ"
     gene            complement(1469..1609)
                     /locus_tag="VFY96_07915"
     CDS             complement(1469..1609)
                     /locus_tag="VFY96_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606424.1"
                     /translation="MSYLNATFEAFQVPQDERDEVVAFVGSTTGIVTQTGEAILRDRP
                     SK"
     gene            complement(1715..2689)
                     /locus_tag="VFY96_07920"
     CDS             complement(1715..2689)
                     /locus_tag="VFY96_07920"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEX5606425.1"
                     /translation="MLNNFLQIVPEVIFDAAEKLGGRCTGRFYTLHALENRVYDIEME
                     EGPHLVIKFYRPGRWSRATIQAEHGFLKALDESEIPVVCPLTDNSGQSIFEITGVMFA
                     IFPKRPGRLEPELSKEQLTRLGRFLARLHSVGATVKAAPRLRLDPQTYGREPLEFLKN
                     GHFVPMELASRFEMIASQICAGIMPRFESAHYILLHGDCHSGNILWNRDDPYFIDFDD
                     MLYAPPVQDIWMLTGGDDEYGKERRRILLEAYQQIRTFDITTLQLIEPLRALRMIHFS
                     AWIARRWEDCAFKIAFPDFGTEHYWQEQIEALALQLEKVQYVTTDLYH"
     gene            2913..3257
                     /locus_tag="VFY96_07925"
     CDS             2913..3257
                     /locus_tag="VFY96_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606426.1"
                     /translation="MKKSTSIITTSLLAASLLVPSVRAYADDNPPGNHRQREALRQDQ
                     RELQELRDRRHDEIREGDRREAREYQDKIRDKRKEIHNDRREIYGNDHDRRYHDRRYG
                     WDRDGRHDRDHD"
BASE COUNT          761 a          871 c          934 g          708 t
ORIGIN      
        1 gtcaccggct ttaccggccc ggaaatttat atcgcgctgc agcgcggcga aatcgatgct
       61 catgccactg gcgccgcccg tttcgtaatc gagcaaccgg agtggatcga aaagaagctg
      121 gtgcatttgc atgcaaccta cacaattccc aaaggccgcc accatccaag attcacgcac
      181 ctaccggatt acgacagctt cgccaagtcg gacaccgagc gcaaattgct tgacatgttt
      241 cgcgcctttc agtatccgcg ctggccctac atttttcctc cgggagcgcc cagggaccga
      301 gtgcagatca ttcgggaggc aatgcgcaag gcttttgccg atccggattt ccccggcgag
      361 tttcgcaggc tcatgggcga cgatcctgcg ccgatggccg gcgaagagct ggaacgcgcg
      421 ataaaggaac tgccgcggga caaagaggtc gtacagctct atcaaaagct ggccggcgct
      481 gatccgctgc cgcaaagatg aagcgataaa tttgccgggc tcaggataag ttttcgatag
      541 ggtgaggcag ggtgaggagt ccaaggcgat cgcgatacgg gtgagccggg aaacgcaagg
      601 caggcaagac aacagattta gcgttgaagg actctttcgc tttggcgaat tccgcaacgt
      661 tgtatcacct gatcgttacg tgctcgggtg cggtgatgtt gctcgggccg gaaaaaaaaa
      721 gtaatttaga cctttataaa ttggacaggt ctaggaggta cgatcgatgg caaatacaac
      781 ggagcgccct ctcgctgatg cgctcaccgc aatcaagacg cgccgttcgg tgaaggaata
      841 ccttcccacc gaggttccgc gggagtggat cgaggagctg ctcgacgccg cgcactgggc
      901 gcccaaccac aaattgaccc atccctggcg gtttcacgtt tttaccggag aggggcgcga
      961 gcgtttggtc gcagcccgac aggcggcagt tcgctgggcc gcggagaaaa aaggcgaacc
     1021 ggtgagtgaa gacgctctta atttcgcacg agcgaagtgt ttttcgtcgc ctgtcgtgat
     1081 catcgtatcg atggtgggtg atgacaagaa aatcgtcgac caggagaatt acgcggcgtg
     1141 ctggtgtgcg atcgaaaacc tgttgcttgc cgccactgcg cgcggcctgg gcagctatcc
     1201 gagcacgggc gattggatcg accaaaactt tgttggccca gtgctgggac tcacggagaa
     1261 agagcggccg gtggcgtgca ttttcctggg gtactccgaa cagaagacga tggccaagcg
     1321 cctacccgtg gagcgccata ccacgtggta cacggaggca gggactaata gccaataggc
     1381 agtggacaaa gagagataac gttcgcgtcg ttgagaatta ggcaagctag aaaaacccgt
     1441 cagatcgttc tcactagttt gaaatcgttc atttgctcgg tcggtcgcgg agaatcgctt
     1501 cgcccgtctg cgtaacaatg ccggtcgtgc ttcctacgaa ggcgacgacc tcatcacgct
     1561 catcctgcgg gacttgaaat gcctcaaatg tggcattgag gtagctcaaa aatgccgccc
     1621 aactgccttc actctgatcc tatttctatc aacacgttca ttaagtgttt ttggttcatg
     1681 tcctccaatt cgccgcgcat cgcaacgatt ccgttcaatg gtagagatcg gtcgtcacgt
     1741 attgcacttt ttccaattgc aacgcgaggg cttcgatctg ctcctgccaa taatgctcgg
     1801 taccgaagtc aggaaaggct attttgaaag cgcagtcttc ccagcggcgt gcaatccacg
     1861 cggaaaagtg aatcatgcgc agcgcccgta acggctcgat cagctgcaag gttgttatat
     1921 cgaaagttct gatctgttgg taggcttcga gcaaaatccg ccttcgctct tttccgtact
     1981 cgtcgtctcc gccggtgagc atccagatgt cctgtaccgg aggcgcgtag agcatgtcgt
     2041 cgaaatcgat gaagtatgga tcgtctcgat tccacaaaat gtttccggaa tggcaatccc
     2101 catgcagcag aatgtaatga gcgctctcga agcgcggcat aatcccggcg catatttggc
     2161 tcgcgatcat ttcgaagcga ctggcgagtt ccatgggaac gaagtggccg ttttttaaaa
     2221 actccaaagg ctctcgtcca taagtttgcg gatcgagccg taaacggggt gcggctttta
     2281 ccgttgcgcc gacgctgtgc agacgagcca ggaaccttcc taggcgcgtg agctgttcct
     2341 tgcttagttc cggttcgagc cgccccggac gcttcggaaa aattgcgaac atcactccgg
     2401 taatttcgaa aatgctttgt ccactgttgt cggtaagcgg gcagacgacg ggaatttcgc
     2461 tttcgtcgag ggccttcagg aaaccgtgct cggcctgaat cgtggcacgg ctccagcgtc
     2521 ccggccggta gaatttgatc acgaggtgcg ggccttcttc catttcgata tcgtaaacac
     2581 gattttcgag cgcatggagc gtgtagaagc gaccggtgca acgcccgccc aacttctcgg
     2641 cggcgtcgaa aataacctcg ggaacaatct gcaggaaatt gttcagcata gaggtcatcc
     2701 tagacgagcg taccctggtt gtacagtaaa ccggacggct acttttcgct ggcgagtgtc
     2761 gctctactgg gacgaatgaa aatttctaga tccagtagag gaattccgtc ggttctggct
     2821 gtgcggttct gcgcaaccgc ctaatatcgt gcgctttacg tctggtatat cgcttgcgac
     2881 ttcccagtgt agtacatcga acggaggtac tcatgaaaaa atcgacatcc atcataacga
     2941 cgagcttact ggcggcatct ttgctcgttc ccagcgtgcg cgcgtacgcc gacgacaacc
     3001 ctcctggcaa ccaccgccag cgtgaagctc tgcgtcaaga ccagcgcgag ctgcaagaat
     3061 tgagagatcg acgtcatgac gagatccgcg agggcgacag gcgggaagcg cgcgagtacc
     3121 aagataagat acgcgacaag cgaaaggaga ttcataacga ccggcgcgag atctacggca
     3181 acgatcatga ccggagatat catgaccgga gatatgggtg ggatcgcgac ggaaggcatg
     3241 accgcgacca cgactgattg taaatctttt cgac
//
LOCUS       DASULZ010000383         3226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_453631, whole genome shotgun sequence.
ACCESSION   DASULZ010000383 DASULZ010000000
VERSION     DASULZ010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453631"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VFY96_07930"
     CDS             <1..363
                     /locus_tag="VFY96_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606427.1"
                     /translation="MKTKAAILTGVLLITGSLAVSTPGFTGDGKDHGKRAERSDRRDR
                     DARDLQGTWYMNGDHNKRTEINDNGRELQGKNEDGKTSRLEVDRRGNVHALDWNGITG
                     HLRGNRIEWDNGTTWTRG"
     gene            1168..1461
                     /locus_tag="VFY96_07935"
     CDS             1168..1461
                     /locus_tag="VFY96_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606428.1"
                     /translation="MERAKAISTERRSDPNQSAVAGAATSSVVGAAAGGAGGAVFGQA
                     AQGAAAGAVGGVVGSLTQALIQGLFRPKPPDLTNQQLVDSCLRQKGYEPAGWK"
     gene            2133..2474
                     /locus_tag="VFY96_07940"
     CDS             2133..2474
                     /locus_tag="VFY96_07940"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HEX5606429.1"
                     /translation="MNGKKGLIILSAIFVIAASSGCSTPLSTREKGAGIGALGGATAG
                     GLIGAAVGHPAAGAAIGGALGLGAGALVGDQLQGQEQTQYRQQQQIDQNQAEIQRQRQ
                     QIDQLKRRQEY"
BASE COUNT          805 a          804 c          914 g          703 t
ORIGIN      
        1 atgaaaacga aagctgcaat attgacgggt gttttgctca tcaccggaag tttggctgtt
       61 tcgactccag ggtttacagg cgatggcaaa gatcatggca aaagagctga aaggtcggac
      121 cggcgcgatc gagatgcgcg cgacctacag ggaacctggt acatgaacgg ggatcacaat
      181 aagcgaacag agataaatga taatggtcgc gagcttcagg gcaagaatga agacggcaaa
      241 acaagtagat tagaagttga tcgtcgtgga aatgttcatg ctctggattg gaacggtata
      301 actgggcatc taagagggaa cagaatcgaa tgggataatg gcacgacctg gacgcgaggg
      361 tgatctgaaa ccacgagttg ttcataaggt caaaaagggc gctggatcaa cccagcgccc
      421 ttttttgggt actttccttg ccatcgtagg tttttgactc tgcgcttcgc ccatctctcc
      481 ggctgcaccg cccgcaatgg tccgtgcgca atggccgaac atgccagtga ttttaaggac
      541 gggttttctt tttcaggatg gcgcaaaaag tatgatgaac ggctgagtga ttttattcca
      601 aaacaatcaa tgccgagaca ttaatcgata tgattcggcg tcgcatacca ctatcaagtt
      661 agcttccaac caatcagttt catgccccct ggtcgagcca ttgataacag cccggccagg
      721 gggtattcaa ggcaattcca ttttccggcg atatactttt cgtgcggtgc tgcagttgcg
      781 gggcctagtt ggagggaaag caacgtctga gaaggaaagc agactccgag tccggtctat
      841 cgctggtgct gggccagcca cgtccattct caacaacctg cgaagcggaa gcccagaaaa
      901 aaaaccgcag aagaaccgtg gaaagcggcg ataccgacat ttacattcgc cctgtacaat
      961 aaaacaataa acgtctatta atcttgaaac gcacagagat aacaacaggt atttttgatg
     1021 ctcacgaact ggacaataag cagtgcgacg attttctcgc tgctactcca actatcgggc
     1081 tgtgccactc agaggccggt gctttcttcg aatgagcatc taatccgcgt cggacccgac
     1141 gttgcgcaac aggatatcga caattgtatg gaacgagcga aagcaatatc cactgagagg
     1201 cgtagcgacc ccaatcagag cgcggtcgcc ggagcagcga cgagcagcgt cgtgggggcg
     1261 gcggcagggg gcgcgggcgg cgcggtattc gggcaggctg ctcagggggc cgctgccggc
     1321 gcagttggcg gggtggtcgg cagcctcact caagcgctca ttcagggatt gtttcgaccg
     1381 aaaccgccgg atctaacgaa tcagcaattg gtggatagct gtcttcgaca aaaaggctac
     1441 gagcctgcgg gatggaaata gtctatcgat cggcgattgc ctcaaacatc gttagaacat
     1501 cgtccgtggt ttcgctgcgc ggccgctgcg gtctagccgg cgcgtctgaa agtgcttgag
     1561 tcctgcggat cccaagacgg catagcgctc actgaccaac cggaaatccg atagcacaga
     1621 ttggcaagca cgttccgtcg ctgctctcga cggaggaagc atcgaaaccg ataaggcagc
     1681 aacccatcac gaacatgccg taagcgctgt ctatatcatc cgggcgtttg atcaaagccg
     1741 attggccttg ttgtttctga acgcgtttgc gatcctgccg aatgccggag tgtgacacga
     1801 caggcgcgag agaacgcccc ggctttcctt tttcgtatat ctaacaataa aaaatcgaaa
     1861 cggtgaatgc gcgcgcaaca tagcgcgacc ggcaccgagc cgcggtacga aattgagcgc
     1921 cactttcaac gcaccgggtg tcagcggcac gattgtttgt ctcaatttgc aaataaattc
     1981 gtagctttct taattagcgg tcggcttgtc atcaggaata tacggtatgc gccttgcgtg
     2041 gtcttcttcc aacttattat gcatcgcaca ggttcgatcc gcaggtcact cagcaaattc
     2101 ggaattggat caagtatagc aaaggagttg tgatgaacgg caaaaaagga ctaatcattt
     2161 tgtctgcgat tttcgttatt gcggcgagct caggctgctc cacaccacta tcaaccagag
     2221 aaaagggagc aggaatcggc gcgctagggg gcgccactgc gggaggtcta atcggtgctg
     2281 cagtgggtca tccagcagct ggagcagcga ttggcggagc gcttgggctg ggcgccggag
     2341 cgctggtggg tgaccaactg cagggacaag aacagactca gtaccgccag cagcagcaga
     2401 tcgaccaaaa tcaagccgaa attcagcgcc agcgacaaca gatagatcag ctcaaacgcc
     2461 ggcaggaata ctaagtttca gttgcgagcg cggctaggaa catagtcgcg cagtgggcaa
     2521 ttctcaggag actgagaaac agattgtctg cgcgagcgag gctcgcccgg gcaggcacgt
     2581 gatcagttgg cgctgactct taccgcttcg gccctacgga tgaatccggg cggtcgcccg
     2641 tgtttcgaac gaatttccag ccagccctct ctggaatcca ctacgttcag tcggactcct
     2701 ttcgcgatgc tggccacgag ggcagaatcc tcgctaggct cgctgaaaac ctgggttgct
     2761 cgaacagtct gaaacgtgcc acggacacgt ggtaacgccg cgatggatgg tcgcggtgat
     2821 acaggcgttg acggctgctc ggcagcagca gtgtccgtgt ggactggagg cgtggaactt
     2881 aaagattcag ttctgaggga ttgtatactt atgccggcca cagctattgc aaggatcgag
     2941 ctggcgaaga tcgcgccaaa acgccacttg tgaagaacca agctcggaag taataggcta
     3001 acccttgtcg ctttacccga actaatcgcg gcatcacgta gtttatggtc ggtaccatta
     3061 gttgctctgg gcaagtctgc gggtaaaaca gcaacggtat tttccgcgtt ttgactgttg
     3121 atcacattgg ccgaatcatg cttacgtgat aataaactat cactactagg ttctctttgg
     3181 agatttagct gcttttccat ccgacgattg ctctgaagta gcgtgt
//
LOCUS       DASULZ010000384         2327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1367598, whole genome shotgun sequence.
ACCESSION   DASULZ010000384 DASULZ010000000
VERSION     DASULZ010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367598"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VFY96_07945"
     CDS             complement(<1..354)
                     /locus_tag="VFY96_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606430.1"
                     /translation="MKLIFGLVLILFSAVASWGAQTAKLQLALNWKAEPEFGGFYTAK
                     LSGYFHKRNLDVAITEGGAGTPVVQMVANSKVRFGIAAADEIVISQARGTDVVALFAV
                     YQVNPQGIMVHPERGF"
     gene            complement(354..1124)
                     /locus_tag="VFY96_07950"
     CDS             complement(354..1124)
                     /locus_tag="VFY96_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5606431.1"
                     /translation="MNLEAKISSLLPPLLPLILSMGFAETAVRLGWAPSYLIPAPSEV
                     IRSLIDDRFELAVAAWTTLSSALAGLLLSFTTGTLFAVALSSSDLARRAFYPYAVFFQ
                     TVPIISIAPLLVIWFGFGQPTVIASAAIVSVFPIIASTLLGLKSTEPALIDLFTLYSA
                     SARQMLYMLKIPFALPQIFSGLRIASGLAVVGAIVGEFIGGGGLGSVVDSARTQQRID
                     RVFAAVLISALLGAVLVGAVNFISALTLGSWHTSERKV"
     gene            complement(1121..1906)
                     /locus_tag="VFY96_07955"
     CDS             complement(1121..1906)
                     /locus_tag="VFY96_07955"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5606432.1"
                     /translation="MNSSSYSDQRKSGAAVRCERISRAFAGTGLVLDRISFQIEAGEF
                     VALLGPSGSGKSSLLRLTAGLDRADAGRLAIEAEQGAVSRGFVFQEATLVPWRNVLRN
                     TTLPLELQGWEMERAKERAQEELRRVRLMEFAARYPHELSGGMKMRTSVARALITRPN
                     LLLLDEPFAALDEPTRHALQQQLREIWAALGMTIVFVTHSISEAVFLANRIIVLSPRP
                     ARILLDSSIQLSETRTAALRTSLSYVEEVRRITTAVPAPEQEI"
     gene            complement(2010..>2327)
                     /locus_tag="VFY96_07960"
     CDS             complement(2010..>2327)
                     /locus_tag="VFY96_07960"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5606433.1"
                     /translation="ERLDEKFEKLPQQAPLLSHEPSHYVRSCPIYFSCELEEKILPYV
                     ISLGLQKRIMYPSDYPHERPTLEEFLADIPRFGERSDLSEETKKRILRDNCIEFYSLK
                     LSD"
BASE COUNT          577 a          674 c          627 g          449 t
ORIGIN      
        1 aaatccccgt tccggatgaa ccatgattcc ctgcggatta acctgatata ccgcaaacag
       61 cgcgaccacg tcggtgccgc gcgcctgcga gattacgatc tcatcggctg cggcgatgcc
      121 gaaacgaact ttactgttgg cgaccatctg cacgaccggc gtacccgcgc cgccttcggt
      181 gatcgcgacg tcgagatttc tcttatgaaa atagcccgac aatttcgctg tgtaaaaccc
      241 gccaaattcc ggctccgctt tccagttgag cgcaagttgc aatttggctg tctgtgcgcc
      301 ccatgatgcg acggcagaga acaaaatgag taccaagccg aaaattaatt tcatcaaacc
      361 ttcctttccg atgtatgcca cgaacccaat gtcaacgccg aaatgaaatt gaccgctccg
      421 acgagcacgg cgcccagcag cgccgaaatc aacactgccg cgaaaactcg atcgattctt
      481 tgctgtgtcc gcgccgaatc aacaacgctg cccagtccgc cgccgccgat gaattcaccg
      541 acgatcgcgc cgaccaccgc gagcccggaa gcgatccgca aaccggaaaa aatttgcggc
      601 aaagcgaaag gaatttttag catatacagc atctgacgcg cactggccga atagagagtg
      661 aacaggtcaa tcagcgcagg ctcggttgac ttgagtccaa gcagcgtact cgcgatgatc
      721 ggaaacaccg agacgatcgc ggcagacgca atcactgtcg gctggccaaa gccgaaccag
      781 atcaccagca gaggcgcgat cgagatgatg ggcaccgttt gaaagaaaac cgcataggga
      841 taaaaagctc ggcgcgccag atccgatgac gacaaggcaa ccgcgaacaa agttcccgta
      901 gtgaaactaa gcagcaggcc ggccaacgcg gagctcagag ttgtccacgc cgcgaccgca
      961 agctcaaagc gatcatcgat taaagaacga ataacttcgc tcggcgccgg gatcaaatag
     1021 gagggcgccc aacccaaacg tacagcagtt tccgcgaacc ccatactaag aatcagcggc
     1081 agcagcggcg gtaaaagaga cgagattttt gcctcgagat tcatatctct tgttctggcg
     1141 ccggaaccgc ggtggtgatg cgccggactt cttcgacata ggaaagagaa gtgcggagcg
     1201 cggcggtgcg ggtctcgctg agctgaatcg aactgtccag cagtatgcgg gctggtcggg
     1261 gcgacagcac aatgatccgg tttgcaagga atacggcttc ggaaatcgag tgcgtaacga
     1321 acacgatggt catgccgagc gccgcccaga tttcgcgcag ctgctgttgc agagcatggc
     1381 gcgtgggctc gtccagggcc gcgaaaggct cgtccaggag cagaagattc gggcgcgtga
     1441 tcaaggcgcg cgcaaccgac gtgcgcatct tcataccgcc ggagagttcg tgcggataac
     1501 gcgcggcgaa ttccatcagg cgaacgcgcc gcagttcctc ctgcgcgcgc tccttggcgc
     1561 gctccatctc ccatccctgc agctcgagcg gcagggtggt atttcgcagc acgttccgcc
     1621 atggaaccaa ggtcgcttct tgaaaaacaa aaccacgaga aacggcgcct tgctcggctt
     1681 cgatcgccaa tcggccggca tccgcccgat cgagtcctgc cgtgagccgc aataacgatg
     1741 atttgcctga ccccgacggc cctagcaaag caacaaactc gccagcctcg atttgaaaac
     1801 taatacgatc gagcaccaag ccggtgccgg caaacgcgcg cgaaattctc tcgcagcgaa
     1861 ccgcggcgcc cgactttctc tggtctgaat acgagctgga attcatcgaa cgcttaggaa
     1921 attgagagcc tcactttact cttttcgagt gaggcactcc agcgttgatg aagaaacgcc
     1981 gactacgccg ggactcgctt cctagtttgt taatcggaca gtttcagcga gtaaaactcg
     2041 atgcaattgt cacgcagaat acgctttttg gtttcctctg atagatcgct tctttcgcca
     2101 aaccgaggaa tgtcagcaag aaactcttca agagtcggcc gctcgtgcgg ataatcggac
     2161 ggatacatga ttcttttctg caatccgagg ctgatcacat agggcaggat cttttcttcg
     2221 agctcgcagg aaaaataaat cggacagctc ctgacgtaat ggctcggctc gtggctcaac
     2281 aatggagctt gctgtggtag tttttcaaac ttttcgtcca gccgttc
//
LOCUS       DASULZ010000385         2295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_859919, whole genome shotgun sequence.
ACCESSION   DASULZ010000385 DASULZ010000000
VERSION     DASULZ010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859919"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VFY96_07965"
     CDS             <1..476
                     /locus_tag="VFY96_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX5606434.1"
                     /translation="HARAYKARNDIQSVAHLHNHMVATLSMVDKPFYPASSNPGAFFG
                     PGRLPVYMDPALIHTIEQGDAVAKTLGTADAVMLRGHGSMVVAHSIEWVFAGCVDLEE
                     AASRFYKASLLGPVMIYNEDETQRVMKQRRKDSVVQKIWDHNAAKTELAGLMNGI"
     gene            747..1145
                     /locus_tag="VFY96_07970"
     CDS             747..1145
                     /locus_tag="VFY96_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606435.1"
                     /translation="MYDDFLARILEPDVFLPAQFYGTGGLSRQLDGEKRLMIAILKDA
                     VECLNKYRGARSSLGSTQYQNALEWVQDEGTDWLFSFNNICDLLGFDPDYLRKVLIKK
                     EARHANPVRGKIYSFPIGSGAGGGSPAARS"
     gene            complement(1125..>2295)
                     /locus_tag="VFY96_07975"
     CDS             complement(1125..>2295)
                     /locus_tag="VFY96_07975"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX5606436.1"
                     /translation="MGPQPFEYRFFVIADPQLNAFAVPGGSVFFYSGLIERAKSTSEI
                     AGVMGHEIVHVKGRHMARHSGPDAISLLSLVGMLLLARSGSGAQAAGVVGQAVSATRQ
                     IAYSRQLEMEADTLGVRYMTAAGYDPAGSLGFLKTLDQERSLNPIDVPAYMLTHPVTQ
                     ERIANVQLVIRSLGDVQSKSESLDQLKKIQVLIRLERQETDKVIDEYQKLVAQSPSQV
                     MALHLLALAQHKKGQLSLAKTNYEKALALNPEDPGLCRDLGRLYTASGDFGAARTTLD
                     RAIAMEPKEPLNYLFLGELLEKQNDLRGAAGAYLNAQQLSPLWDKPPARLGIVYGNLD
                     RLGDAYYYRARSLLLQDEDERAMAAFEKAIKILGENSARGQTAKEELNNLRARRR"
BASE COUNT          527 a          593 c          621 g          554 t
ORIGIN      
        1 tgcacgcgcg cgcctataaa gcccgtaacg atatccaaag cgtcgctcat ctgcacaatc
       61 atatggttgc caccctaagc atggtcgaca aaccgttcta tccggcatct tctaatcccg
      121 gcgcattttt tggcccgggg cgcttgcctg tttacatgga tccagcgctg attcatacga
      181 tcgagcaggg tgacgcagtg gcaaaaacgc tggggacggc ggacgcggtc atgctgcgcg
      241 gacatggctc gatggtagtc gcccactcta tcgaatgggt atttgccgga tgtgtggatt
      301 tggaagaagc cgcatcgcgg ttctataaag cttcgttgct gggacccgtc atgatttaca
      361 acgaggacga gacccaaagg gtcatgaaac agcggcgcaa agactcggtg gttcagaaaa
      421 tctgggatca caatgctgct aagacagagc tcgccggcct gatgaacggg atctaaacac
      481 cagtaagatc gccggtttcg aagcgcatcg tgggattgtg atagggcgga gcccgaaggc
      541 cggatttatc tttctatgct ccagatgatg tcgtccttcc ctgcccttcg aggtgaatgg
      601 gagctcaact ggacataaat ctgctcttaa ctttactggc agcgctgcgt tatagtcctt
      661 ggcgacttta tgatgatatt tgttgcgcgg aagaaactcg cgtgatatag ctggcgattc
      721 aatttcatcc aggagtgttt tcgcatatgt acgatgattt tcttgcaaga atccttgagc
      781 ctgatgtttt tctgccggct caattctatg gcaccggagg cctgtcacgt cagctcgatg
      841 gtgagaaacg attgatgatt gctattttga aggacgccgt cgagtgtctc aataagtacc
      901 gcggcgcgcg aagtagttta ggaagcactc agtatcagaa tgccttggaa tgggtccagg
      961 atgaaggcac cgactggctg ttttcgttca acaacatttg cgatcttctg gggtttgatc
     1021 ccgactatct tcggaaggtg ctgatcaaaa aagaagctag acatgcaaat cccgtccgtg
     1081 ggaagatcta ttcttttccc atcggcagcg gagccggtgg aggctcaccg gcggcgcgct
     1141 cgtaaattat tcagctcttc ttttgcagtc tgcccgcgtg cagagttttc gccgaggatc
     1201 ttgattgcct tttcaaaagc ggccatggcc cgctcgtctt cgtcctgtag caacaaggac
     1261 cgagccctat agtaataggc gtctccaagc cgatctaggt tcccgtagac aatgccaagc
     1321 cgggcaggcg gcttatccca taatggtgag agctgctgag cgttcaggta tgcgcctgcc
     1381 gctccccgta agtcattttg tttttcaagt agctctccga gaaacaaata attaagcggt
     1441 tccttgggct ccatggcgat ggcacgatca agtgtagtac gggccgcgcc gaaatcgccc
     1501 gacgcggtgt ataggcggcc gagatcgcga cacaaaccgg ggtcttccgg attgagtgcc
     1561 agcgccttct cgtaattcgt cttcgctagt gaaagctggc cctttttatg ctgcgccagc
     1621 gctagaagat gaagcgccat aacttgtgac ggagactgcg cgaccaattt ttgatattcg
     1681 tcgatcacct tgtcagtctc ttgccgctct aaccgaataa gaacctgtat ctttttaagc
     1741 tgatcgagac tttcggattt actctgaaca tcgccgagtg agcgaatcac caattgaacg
     1801 ttagcaatcc gttcctgggt tactggatgc gtcagcatgt aggccggcac gtctatcgga
     1861 tttaaagatc gttcctgatc taaggtcttg agaaagccaa gcgaacccgc agggtcatag
     1921 ccggccgctg tcatatagcg cacacccaaa gtgtcggctt ccatctccaa ttgccggcta
     1981 tatgcgattt gacgagtagc agaaacggct tggccaacga cgccggcagc ttgtgcgccg
     2041 gagccgctcc gggccaacag aagcattccc accaaactca aaagtgaaat cgcatcgggg
     2101 ccggagtggc gcgccatgtg acgccctttg acgtgaacaa tttcatgtcc catgactccg
     2161 gcgatttcag acgtgctttt cgcccgttcg ataaggccgg agtaaaagaa aaccgaaccc
     2221 ccaggcaccg caaacgcgtt tagctggggg tcggcgataa caaagaaccg atactcgaaa
     2281 ggttgcgggc ccatt
//
LOCUS       DASULZ010000386         2190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_351878, whole genome shotgun sequence.
ACCESSION   DASULZ010000386 DASULZ010000000
VERSION     DASULZ010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351878"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1592)
                     /locus_tag="VFY96_07980"
     CDS             complement(<1..1592)
                     /locus_tag="VFY96_07980"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5606437.1"
                     /translation="MPLVGTDVAMVGGLAKVTGAVNYSPDLVLPRMLYAKALRSPYPH
                     AKLLRVDASKAEKYSGVVAVVTHEDLTGLNPYFGPVVDDQPVVATDRVRHIGEVVALV
                     AAESREIAEEALDLIEVDYQELPAVFDLLEAAKPGAPVLHEQRNETNAGVHREEFNFE
                     LGGNVCSVFRVADGNIQKGFQEAEEIFENTYVLPPVQHGHIEPHAATAYWEPDGKLVV
                     HSASQNPSGVQEQLAHIFDLPENKVRVIVPILGGGYGGKTHARLEPALALLTRKARRP
                     VQWVLTRDEVFLTGRRYGAVVKIKTGVKRDGDIVARQVEVFYEIGAYALNGPVNAKTG
                     CYVSSGPYNIPNRSLSTYSVYTNLPPTGPFRGVGVSHVCWAYESEMDDIARRLGLDPL
                     ALRLKHLVKEGDVFVTGEKLVSVGITDCLERAAAAIGWKGKEEQSARPDGNNLIRGKG
                     LAVTIKSTTTPTTSSANVRLNADGSAILLTSSVDMGQGALTSLAQIVGDELGLPFEKV
                     SVSSPDTDITPYDKSTSSSRTT"
     gene            complement(1606..>2190)
                     /locus_tag="VFY96_07985"
     CDS             complement(1606..>2190)
                     /locus_tag="VFY96_07985"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX5606438.1"
                     /translation="EVLAEACHVVANIRIRNMATIGGNLAHGDYQSDPPTVLVALEAV
                     VELLSRTGMRRMKLSDFLKGSYETAIEPGELVSALIVPPADHLRGTYTKFTTGSSEER
                     PCAGIAALASMDDSVCLELRLVVGAVSPKPVRMQNGESMARGERLTQEMVERIAKDAS
                     RNVDPIDDLRGTADYKRHLVHVLTRRALSALAGV"
BASE COUNT          450 a          660 c          602 g          478 t
ORIGIN      
        1 aaagttgtgc ggcttgagct cgtcgattta tcgtagggcg tgatgtcagt atccggagag
       61 ctcaccgaga ctttctcaaa cggcaatcct agctcgtcgc cgacgatttg cgccagcgaa
      121 gtcaatgccc cctgccccat atcgacgctg ctggtaagaa gaatagctga accgtcggcg
      181 ttcagacgca catttgccga cgacgtcgtc ggcgtggtcg tgcttttgat cgtaaccgca
      241 agccctttgc cgcggattag gttgtttcca tccggtcgtg ctgattgttc ttccttccct
      301 ttccagccga tcgctgcagc ggcgcgctcc aaacaatcgg tgatgccgac agaaaccagc
      361 ttttccccgg tgacgaatac gtcaccctct ttaacgaggt gcttcaggcg caacgccagc
      421 ggatcgaggc caagccgccg cgcgatatcg tccatctcgg attcgtaagc ccagcagaca
      481 tgagaaacgc cgacaccgcg aaacggtccg gtgggtggca aattcgtgta aacagagtac
      541 gtcgaaagac tccggttggg aatattgtag ggaccgcttg agacataaca gccggttttg
      601 gcgttgaccg gcccgttgag cgcataagcg ccgatttcat aaaagacctc gacttgacgc
      661 gcgacgatgt caccgtcgcg ttttactccg gttttgattt tcaccaccgc gccgtagcgt
      721 ctgccggtga gaaagacctc gtccctggtc aaaacccact gcaccgggcg gcgcgccttg
      781 cgtgtgagta gcgccagagc gggctccagc cttgcatggg tcttgccgcc gtagccgcca
      841 ccgagaatgg gaacgatcac gcgcacctta ttttccggca aatcaaaaat atgtgccagc
      901 tgctcctgca cgccggaagg attttgcgag gcggaatgaa ccaccaattt gccgtccggc
      961 tcccaatagg cagtggccgc atgcggctct atatgaccgt gctgcaccgg cggcagcaca
     1021 taggtattct caaaaatctc ttcggcttct tgaaaacctt tttggatgtt gccgtcggcg
     1081 acgcggaaca cgctgcacac gttgccgccc aactcgaaat tgaattcctc gcggtgcacg
     1141 cctgcattgg tctcattgcg ctgctcgtgc agtacgggcg cgccaggctt ggcggcttcg
     1201 agaagatcga atactgccgg caactcttgg tagtccactt cgattaaatc cagcgcctct
     1261 tcggcaatct cgcggctttc cgcggcgacg agcgcgacga cttcaccgat atggcgcacg
     1321 cgatcggtag cgactacggg ttgatcgtcg accaccggtc caaaataggg atttaagccg
     1381 gttaaatcct cgtgagtgac gacagcaacg acgcccgaat atttttccgc cttgctcgcg
     1441 tccacccgca aaagctttgc gtgcggatac gggctgcgca gcgccttggc atagagcatg
     1501 cgcggcagta cgagatcagg tgagtaattc accgctccag tgaccttggc cagccctcct
     1561 accattgcta catctgttcc gaccagaggc attcttcgtt ccttcttaga ctcccgccag
     1621 agcgcttagc gcccggcgag ttagcacatg aaccaggtgg cgcttgtaat cggcggtgcc
     1681 acgcaagtca tcgatcggat caacgttacg gctggcatct ttggcgatgc gctcgaccat
     1741 ctcctgtgta agccgctctc cgcgcgccat agactcgccg ttttgcatcc gcaccggttt
     1801 aggcgatacc gcgccgacta ccaaacgaag ctccaagcaa acgctgtcgt ccatgctcgc
     1861 taatgcggca attccggcgc agggccgctc ttccgatgag ccggtggtaa atttagtgta
     1921 ggtgccgcga agatgatcgg ccggcggaac gatcaaggcc gacaccaatt ctcccggctc
     1981 gatcgcggtc tcgtaactgc ctttcaagaa gtcggataat ttcatccggc gcattcctgt
     2041 gcgacttaaa agttcgacga cggcctccag cgccactaga accgtgggcg gatcggattg
     2101 atagtcgccg tgcgccagat tgccgcctat ggtagccatg ttgcgaatgc gaatgttcgc
     2161 aactacatgg cacgcttcag cgagcacctc
//
LOCUS       DASULZ010000387         2895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1164745, whole genome shotgun sequence.
ACCESSION   DASULZ010000387 DASULZ010000000
VERSION     DASULZ010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164745"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="VFY96_07990"
     CDS             <1..986
                     /locus_tag="VFY96_07990"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606439.1"
                     /translation="IVGFFFFGETVDAASAPAKSTIAHAAVNARVLPLWVARDQGFFP
                     KYGVPSETIFIRQAPTLVAALTSGDIQIGYTGGTAVLGAAAGGSDLKILAAFTNRVTY
                     DVVAKKNIKTPEDLRGKIFGVQSIGGTVWMGAILALEHFGLDPNRDKISLIAAGDQSV
                     LAQALVAGTIDVTVLDGVMSRTLRDNGYTILAELSKSNIPISSVGIVAKESFIQSSPQ
                     TIENLLKALLESEAFIFGPSNKSRAMAILKKYLRVNDQEAEEGYKDVITGLDRKPHAS
                     LDGLRNVQRLMKLRNPAVEKVKVEELVDDRFMKKLDQSGFIDELYAKYGVK"
     gene            1133..2245
                     /locus_tag="VFY96_07995"
     CDS             1133..2245
                     /locus_tag="VFY96_07995"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5606440.1"
                     /translation="MYIDGDTHYWPLRFIEKVKHPGRGHLEVVEDKGDMVRYGEVVPG
                     KVATYYRDGKKIHSFKEGRWSIPLRAEFMKKDGFDVQVLIPDNRPLIYECDAELGRQL
                     ARAYNDTVAEDIKGDNRFIGVAWIYLPDIQESIKELRRAVKELGLRAVKFNGGWGDGD
                     LDNEVLFPLYEEIADLDIPILLHPAARVFELQHSHPWLVGAERFQGFQHFPTALGFPL
                     TYMVSAARLIFSGTLDRFPTLRFGFFEGGIGWVPWLMATLNAHTPGEAGVSVAVQQFF
                     EGKQAIKKAPSEYFKQFYIAAVSWESYLADTVNGWRDHNIIIGSDFDHGDAVATWPRT
                     VDVIKRMSNLSADDKEKVLGENAMRMFKLNGSTTMH"
     gene            2568..>2895
                     /locus_tag="VFY96_08000"
     CDS             2568..>2895
                     /locus_tag="VFY96_08000"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX5606441.1"
                     /translation="MAQHILRIDCADEPGLVHKITGVVFNAGYNILSNQEFVDLEAKH
                     FFMRTAFEGPTAPDRVVSYLEQILPGNATVKLTGAERQPIVVMATSEAHCLGDLLIRH
                     YSGELPA"
BASE COUNT          674 a          732 c          821 g          668 t
ORIGIN      
        1 caattgtagg atttttcttt ttcggggaaa ccgttgatgc agcgtcggct ccggccaaga
       61 gtactattgc gcacgccgcc gttaacgcac gggttctgcc actctgggtg gcaagggatc
      121 aggggttttt tcccaaatac ggcgtaccgt ctgaaaccat cttcattcgg caggcgccaa
      181 cccttgtcgc ggcgctgact tcaggggaca ttcaaatcgg atacaccgga ggcacggcag
      241 tgttgggagc ggcggccggc ggttccgatc tcaaaattct agccgctttc accaatcggg
      301 ttacatacga tgttgtagcc aaaaagaaca tcaagactcc cgaagatctg cgtgggaaga
      361 ttttcggcgt gcaaagtatt ggtggcacgg tttggatggg agctattctc gcattggaac
      421 atttcgggct tgatccgaac cgggacaaga ttagtctgat cgctgcgggg gatcaatcgg
      481 tgcttgctca agcgctagtg gcgggaacga tcgatgtaac agtgctcgac ggcgtgatga
      541 gccgcacgtt gcgtgacaac ggttacacga tccttgcgga gctgagtaaa tctaatattc
      601 cgatttcgag cgtgggcatc gtcgccaagg aaagttttat tcagagcagt ccacaaacga
      661 tcgaaaatct gctgaaggcg cttttggaaa gcgaggcgtt tatcttcggc ccttctaata
      721 agagcagagc gatggctatc ctaaagaagt acttgcgggt taatgatcaa gaggcggagg
      781 aaggctacaa ggacgtgatc accgggttgg accgcaaacc gcacgcgagc ttggacggat
      841 tgagaaacgt ccagcggctg atgaaattac gcaatccggc cgtcgaaaag gtcaaagtcg
      901 aagaactggt ggacgaccgc ttcatgaaga aactcgatca gagcggtttt attgacgaat
      961 tgtacgcgaa gtatggagtc aagtgaccca ggtgttttag ccgggtcatc ccgagccaag
     1021 ggcgagggat ctttcttgtt gttcgaccca gccctggttc tggaaagtac gagaaagatt
     1081 tctccctttg gtcgaaatga cagttactaa cacaccaata acgaggattc cgatgtacat
     1141 tgacggtgat acgcattatt ggccgctgcg ttttatcgag aaagtgaagc accctgggcg
     1201 cgggcatctc gaagtcgtag aagataaggg cgacatggtg cgttatggtg aagtcgttcc
     1261 cggcaaagtg gcgacctatt atcgcgacgg caaaaagatt cactctttta aggaagggcg
     1321 gtggagcatc ccattgcgcg cggaattcat gaagaaggac ggattcgatg ttcaggtgct
     1381 gattccggac aatcggccat tgatttacga gtgcgatgcc gagctcggac gtcagttggc
     1441 gcgcgcgtac aacgatacgg tggctgaaga tattaagggc gacaaccggt ttatcggcgt
     1501 ggcctggatc tatctccccg acatccagga gtccataaaa gaactgcgcc gggcggtgaa
     1561 agagttgggc cttagggcag tgaaattcaa cggcggttgg ggcgatggcg atctcgacaa
     1621 tgaagtgttg tttccgctct atgaggagat cgccgacctc gacattccga ttctgctcca
     1681 tcctgccgcg cgcgttttcg agttgcagca cagccatccc tggctcgtcg gcgccgagcg
     1741 ctttcaaggc tttcagcatt ttcccaccgc gctcggcttt cctctgacgt atatggtcag
     1801 cgcggcacgt ttgattttca gcgggacctt ggatcggttt cccacacttc ggtttggctt
     1861 tttcgagggc ggcatcggct gggttccctg gctgatggcc accctcaacg cgcatacgcc
     1921 aggcgaagca ggtgtttccg tggccgtgca gcaattcttc gaaggcaaac aagcgataaa
     1981 aaaagcaccc agcgaatatt tcaagcagtt ctatattgcc gctgtgtcct gggaatctta
     2041 tctcgccgat accgtcaatg gctggcgcga tcacaacatc atcatcggca gcgacttcga
     2101 ccatggcgac gccgtcgcga cgtggccaag aacggtcgac gtgattaaac ggatgagcaa
     2161 tttatcggcg gacgataaag agaaagtcct cggcgaaaac gccatgcgaa tgtttaagct
     2221 gaacggcagc acgacgatgc actgaagtgc cttcgtaaca atctgctaat ccatttcgtt
     2281 ctgagccatt ctgttggcga cttgctcacg tcctgcatcc caccgccatt ccgcaataga
     2341 ctgtttctcc agcgacgaga ctgcgcacaa tctaaaaacg caagcttgtc atgctgagcc
     2401 ttagcgaagc atctcaacca ttttaaccag ctgagaatac aagttcttcg gcggggttta
     2461 actgagcgat gtcgcagcgc ctcaggaacg gcggtccctc ggcgtccgcg attttttctg
     2521 cgtttatatc gtgcaatact gacccacttt actaacggag agcaaaagtg gcccaacaca
     2581 ttcttcgcat cgactgtgcc gacgagccgg gattggtgca caagatcacc ggcgtagttt
     2641 tcaatgccgg atacaacatt ctcagtaacc aggaattcgt cgatttagaa gccaagcatt
     2701 tttttatgcg cacggccttc gaagggccga cggcgccgga tcgggtggtt tcttacctcg
     2761 agcagatttt gccgggtaac gccacggtga agctgactgg ggccgagcgc cagccgatcg
     2821 ttgtcatggc caccagcgaa gcccattgcc tgggtgatct gctgatccgc cactattcgg
     2881 gagagcttcc ggcga
//
LOCUS       DASULZ010000388         6163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_49097, whole genome shotgun sequence.
ACCESSION   DASULZ010000388 DASULZ010000000
VERSION     DASULZ010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49097"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VFY96_08005"
     CDS             complement(<1..706)
                     /locus_tag="VFY96_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606442.1"
                     /translation="MRIAAVLTFILLIIHGAAAAQSEPTEVAPPLEVQDVATLMSPYS
                     EFEGIRKDLAQPTTQTFSLSLIPKWFDLKAFYVEKRQAATPSLYSSLEPNSAPSNLGQ
                     YFDVLASSSHFDGKLVGEGELAYSTLGFAGAPDDRPTMSRLGIRGSWNKLSYGLSYRA
                     FGSGFISTTGVKVDRARDENEIWSEYDFQLFRLRTTLSEWREETANQLLLTRTAATTF
                     NWSKPTWSASLLSSYSL"
     gene            1602..2036
                     /locus_tag="VFY96_08010"
     CDS             1602..2036
                     /locus_tag="VFY96_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606443.1"
                     /translation="MAVDKFRDLAAFGIAALLVGVLLIPFGISRIQGAESQPIRTAES
                     RPIRTKEISSVELPRRPLTVGKSIPLVVHGIALGTAVVYNNPSTGKPSDYLEFYNPTG
                     NLVAVVWFDRFGIRRTAVDRSFLHEKNQIEGVFVPVEDGELI"
     gene            complement(2152..2328)
                     /locus_tag="VFY96_08015"
     CDS             complement(2152..2328)
                     /locus_tag="VFY96_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606444.1"
                     /translation="MFNARLYIDDRLIVDKHGMLDRALLHHPEVLEVASQFGDPYKVL
                     APVSHEAHGSGTAW"
     gene            complement(2292..2960)
                     /locus_tag="VFY96_08020"
     CDS             complement(2292..2960)
                     /locus_tag="VFY96_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606445.1"
                     /translation="MHSPTSRLARSAGFAREGADCAIIDGRLSKAEQLAAEIGALGRR
                     SLARARCDQESSDVVIWVWPVFITFTPAHRAMGRKREESGSQLHEVPLKPYHIYFEEN
                     AGLLARDYAKFPNEVLWKLAEKVREVVSASKVVITEVDGGGEVGEECKRLCKEVPESN
                     RLIEIMFGYHPKASAQHGIADPLHWELTAKCPGPAWEPRANIPSSGIWMDQCSTRAFT
                     STIG"
     gene            complement(3028..3450)
                     /locus_tag="VFY96_08025"
     CDS             complement(3028..3450)
                     /locus_tag="VFY96_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX5606446.1"
                     /translation="MKLSLEKASIILDRALAKAREANYRPMCVAVLDEGGCLRALKRE
                     DGASILRPNIAIGKAWGAVGMGESSRALSERLKERPAFLGALSDMSGGKVVPVAGGVL
                     IVDGSEIVGAVGVSGGTADEDEACAIEGVKAAGLTPKV"
     gene            3629..3907
                     /locus_tag="VFY96_08030"
     CDS             3629..3907
                     /locus_tag="VFY96_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606447.1"
                     /translation="MAIKPFHDVSGDNSSPTVDLESLINDALGCFCREQFCHRRFESG
                     IRVISVVRRGSLIDEKARGFQFGDHFGEIELNALELGDSASELPRARE"
     gene            complement(4421..4696)
                     /locus_tag="VFY96_08035"
     CDS             complement(4421..4696)
                     /locus_tag="VFY96_08035"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5606448.1"
                     /translation="MNVPESDQIDELRAHLRSWSERNPRFWQTQEEKLEDQATSLARL
                     LGQEGFMSYVVPRSYGGTRETGQARDVCVIREELAQFGSLADVMFGV"
     gene            complement(4753..4953)
                     /locus_tag="VFY96_08040"
     CDS             complement(4753..4953)
                     /locus_tag="VFY96_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606449.1"
                     /translation="MGQSPQSRPLYALGVTKEMLETEANLTLGAALEIEAATQAHCMN
                     TADFAEGYNAFVEKRPLQFNRS"
     gene            complement(4970..5362)
                     /locus_tag="VFY96_08045"
     CDS             complement(4970..5362)
                     /locus_tag="VFY96_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5606450.1"
                     /translation="MLRRSVHGIIGPLLKMTNAERYQFTRMTCNLVKNMRQLKKPILA
                     AVNGIAAGADAMLMLASDLRIFSDQARVTFLFVKVGLSGANMGALHLLPRIVGLGKAT
                     ELVSSAIPLMPLRLIASVWLIALCRRTN"
     gene            complement(5650..>6163)
                     /locus_tag="VFY96_08050"
     CDS             complement(5650..>6163)
                     /locus_tag="VFY96_08050"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX5606451.1"
                     /translation="YGTHFMRLKEPRSTLGNFLTVTKRLKQTLGPILVQLRPRWNVNA
                     ERLDEFLAAAPRSLRFAVEFRDERWLCPEVFKVLERRHAALCIHDMLKNHPRLLTAEW
                     TYLRYHGDHYSGSYSEEQVSGEAEWINRQLASGLDVFAYFNNDAHGYAVTNAQELRSY
                     IKPQFLPRPA"
BASE COUNT         1382 a         1678 c         1665 g         1438 t
ORIGIN      
        1 tcagcgagta actcgacaaa agcgatgcgc tccacgtcgg tttgctccaa ttaaatgtgg
       61 tcgccgcggt acgtgtcagt aaaagctggt tggcggtctc ttcgcgccat tcgcttaacg
      121 ttgttcttaa gcggaacagc tgaaagtcgt actcactcca gatttcattt tcgtcgcgcg
      181 ctcggtcgac cttgacgccg gtagtggaaa taaatccgct gccaaaggca cggtacgata
      241 atccataact caatttattc cagctcccgc gtatcccaag ccgcgacatc gtgggacggt
      301 cgtcgggcgc gccagcaaaa cctagtgtgc tgtacgcaag ctcgccttcg ccgacgagct
      361 tgccgtcgaa atgcgaactg ctggcgagaa catcaaaata ctgtcccaag tttgacggag
      421 cggaattcgg ctccaaactc gaatataggg aaggcgtagc cgcctgtcgt ttttcaacgt
      481 aaaaagcctt taaatcaaac catttaggta tcagacttag cgaaaaggtt tgtgttgtgg
      541 gctgagctag gtctttgcga atcccttcaa actccgaata gggcgacatc agggtcgcga
      601 cgtcttgcac ctcgagaggc ggtgcaacct ccgttggctc tgattgcgct gccgccgctc
      661 cgtgaataat aagcaaaata aaagtaagta ctgcagcgat tctcatttag ctttccgcac
      721 gcaagcttta caagtgcgat gttccgtgct gagcccttat agaaaaagtc gaagatgaat
      781 gccgtacgag gttgccgaaa tggccagcgg attaagcaaa aatccaacag cgatgctaca
      841 gcggttcttt ttcagtgtcc agttttttct ccctcactgt gtacgtggtg attatttgtg
      901 ggttgtcctg attcgacctg atcatatttg tcaaagatcc tgttttgagc agagttgtgc
      961 gttatcatcg cagccgtcga ggaattacgg gtaatcatct gtactgatga tcagatttgg
     1021 gcaaaatcgg cgagctcgtt gtgatcgcgc cgcgtcaatt tgcccaggaa atggccgcta
     1081 cgcttctcga gcgaaccaaa agaagaacgc agaacgcaaa caagggaggt gatgttcgat
     1141 ccgattgatt atatcaacgc tggtcagcgg gggcgcgctg cgatgtacgg tttaacagtg
     1201 atttccggct gacttgttcc ggtcgaggtt tttcgaaata cccgccgcca cttagccaag
     1261 aacggttgat cgacttgacc taacggtggt gacatcaatc cgttgacaag gcatcgcgat
     1321 cagctgctat acccgggtag tatgagccgc ctcgcgcgac cgagcgctga gcgtgactcg
     1381 ggaggtgttc atttggagca gaataccaaa cctttaaatt cggccgtctg gagacctctg
     1441 tctcgaaaat ggcacacccc aagacttggc cttttaaata gtgcagtgtt ttcgggcgct
     1501 ctcaagccat ttcgtttttg gcaccatgat tgcttttcgg tggtcggcag tattacacca
     1561 gcacgccatt tcacccctga gatcacatcg aggaggagcg aatggcagtc gataaatttc
     1621 gagacttagc ggcatttggg atagcggcct tgttagtggg cgtcctgctc atcccattcg
     1681 ggatcagtag aattcaaggc gcggaaagcc aaccgatacg gacagcggaa agccgaccga
     1741 tacggacaaa ggaaatatcc agcgtggagc taccacgacg gccgctgact gtaggaaaat
     1801 ctattccact cgttgtccat ggcatagcgc tcgggactgc agtggtttac aacaatccga
     1861 gcacaggaaa gccaagcgac tatctcgaat tttataatcc caccggaaat ttggttgcag
     1921 tcgtttggtt tgatcgcttt ggcatacggc gaacggctgt cgatcgatcc tttttgcacg
     1981 agaaaaatca gatagagggt gttttcgtgc cagtcgagga tggagaattg atctaatccg
     2041 caggccataa tcctagtaag cttagcaggt tcagatctaa ggcaagcgaa tgcagatact
     2101 atcgctggat aaaaagtaga ccgaactgat caagctctac aatataatcg ttcaccacgc
     2161 cgttcccgag ccgtgtgctt cgtgggaaac gggcgcgagc actttgtatg gatcgccgaa
     2221 ttgggatgcg acctcgagca cttccggatg atggagcaat gctcggtcga gcatgccatg
     2281 cttatcgacg atcagccgat cgtcgatgta aaggcgcgcg ttgaacactg atccatccat
     2341 atgccggaac ttgggatgtt tgcgcggggt tcccaagccg gaccagggca ttttgctgtc
     2401 agttcccaat gcaggggatc ggcgatcccg tgctgcgccg aagctttcgg atgatagccg
     2461 aacatgattt cgatcaagcg gttcgattcc ggcacttctt tgcagagacg cttgcattct
     2521 tcacccactt cgccgccacc gtccacctcg gtgatcacga ctttactggc actgaccact
     2581 tcgcgcactt tctcggccag cttccacagc acttcgtttg gaaatttggc atagtcgcgc
     2641 gcaagcagcc ccgcgttttc ctcgaagtaa atatggtaag gtttcaacgg gacctcgtgg
     2701 agctgactgc cgctttcctc tcgcttgcgg cccatggcgc gatgcgcagg cgtgaaggtt
     2761 atgaacaccg gccataccca aatgacaacg tcggagcttt cttggtcaca tcgagcgcga
     2821 gcaagcgagc gccgtcccaa cgcgccaatc tccgcggcga gctgctccgc tttgctcagt
     2881 cgtccatcaa tgatagcgca gtcggcgcct tcgcgggcga atcccgcact tcgcgctagt
     2941 cgggaagtcg gagaatgcac accgttctga gttatgcgac caagagcgct gccgtatcga
     3001 aagatcgtga cgctactcgg acgacaatca gactttcggt gtcagtcccg cagcttttac
     3061 cccttcaatc gcgcaggctt cgtcctcatc cgccgtgccg ccgctgacgc cgacggcgcc
     3121 gacgatttca ctgccatcta cgatcagcac gccgccggcc accggaacga ctttgccgcc
     3181 ggacatgtcg gatagagcgc ccaagaaagc gggacgctct ttgaggcgct cgctcagtgc
     3241 ccggctggac tcgcccatgc cgacggcgcc ccaggctttg ccgatggcga tattcggccg
     3301 caaaatactt gcgccgtctt cgcgcttgag agctctgagg cagccgccct cgtcaagcac
     3361 ggcgacgcac atcggtcggt aattagcttc gcgtgctttg gcaagtgctc gatcgaggat
     3421 gatggatgct ttttcgagag acagtttcat ttggatctcc ttgaataatt agctcagcgt
     3481 ctttttaagg gcgacgatct tatgcccgac tcgcgtcata caatatttca acgaccgtac
     3541 cgatcttcac agctatttga gcaattcccg cgcgatcagc aacttttgaa tctcggatgt
     3601 cccctcataa atccggaggg cgcgcacgtt ggcgataaag ccgttccatg atgtgtccgg
     3661 cgacaactcc agtcccaccg tggatctgga gagcttgatc aacgatgcgc tgggctgctt
     3721 ctgtcgcgaa cagttttgtc atcgacgatt tgagtcggga atccgcgtca tttcggtcgt
     3781 gcgccgtggc agcttgatag acgagaaggc gcgaggcttc caattcggtg accatttcgg
     3841 cgagattgaa ttgaatgcct tggaactcgg cgatagcgcg tccgaactgc cgcgggcgcg
     3901 tgaatagctg atcgcttcgg ccattgcccg tcgcgcaaaa ccgactgcac tcgctcccac
     3961 agtgcatcga agaaggtcga gggtttgcaa ggcgattttc aatccgtttc cttctcttcc
     4021 gagcaactga ttttgcggca caaagcactc ctcgaactga agcacgccga tcggatgcgg
     4081 agacaaaaga cgcgttttct ccgtcacctt aaaaccggcc gaatccgcct ccacgaccaa
     4141 ggcgctgatt ccttttccct tggcgttcaa gtcggtggag gcaaagacaa cgtaaacctg
     4201 agcgacaccg gcatttgaaa tgaacttttt actgccggtt atacagtatc cgccgctgac
     4261 cttgttggcc cggagactaa tggccgcaac attggagccg gccggcttcg gcctccgtca
     4321 aagcgaaagc tccgatgttt tcaccgcgcg ctaacaaggg caagtatttc ttttcttgtt
     4381 tttccgtgcc tgcaataacg atcggctgac tggcgagcgc ctaaacgccg aacatcacat
     4441 cagcgaggga gccgaattgc gcgagttcct cacgaatgac gcatacatcc cgagcctgac
     4501 cggtttcgcg agtaccaccg tacgaacgcg gaaccacata ggacatgaaa ccttcttgcc
     4561 caagtaggcg tgcaagggat gtagcttgat cctccagctt ttcctcctgc gtttgccaaa
     4621 accgcggatt tcgttccgac caggagcgca agtgcgcgcg gagctcgtca atttgatccg
     4681 attccggaac gttcatttcc ttaaagaaat cggtaagcag tacaacatgc gacggctatg
     4741 gtgctgtatt gatcagctgc gattgaactg caacggccgc ttttcgacga atgcgttata
     4801 gccctcggca aaatctgcgg tgttcatgca gtgggcttga gtcgcggctt cgatctccaa
     4861 cgcggcgccc agggttaagt tcgcttccgt ttcgagcatt tctttggtca ctccaagggc
     4921 atacaacggc cggctttgag gcgattggcc cattcgtacg tttcatccat caatttgtcc
     4981 gccggcacaa cgcgattagc caaaccgatg cgatgagcct cagcggcatc aatggtatcg
     5041 ccgaagaaac caactccgtc gctttaccga gacccactat tctcggcagc aagtgtagtg
     5101 cgcccatatt cgctccggac agcccgacct taacgaagag aaatgtcacg cgagcttgat
     5161 ccgagaagat gcgcagatcc gaggccaaca tcagcatcgc gtcggctccg gcagcgatgc
     5221 cattgacagc cgcaaggatc ggctttttca gctggcgcat gtttttcacg agattgcatg
     5281 tcattcgcgt aaattggtac cgctcggcgt tggtcatttt aagcagcggg ccgatgatgc
     5341 catgcacact tcgccggagc aaaacccttt tccggttccc gtgatcacga ccactttggc
     5401 attgcggtcg tgcgccaaat cagccatcac gttctcgttg gcgataggtt tcgaaggtga
     5461 gcgcgtttag tttttcgggt tcgttcagac gtatcgttgc aacgccatca cggctttcga
     5521 aaacgaagct ggaatagctc atggcagctc cttgtcgccg gctttagaac gacgatacgc
     5581 ggtcccattg actaatcaat agccgaaact ctcgcccggt tgagcgaaga tggagctgag
     5641 aaaaattcct caggcgggac ggggtaaaaa ctgcggcttg atgtacgagc gtagttcttg
     5701 cgcgttggta accgcgtagc cgtgggcgtc attattgaaa tacgcgaaga cgtcgagccc
     5761 gctggctagc tgtcgattga tccattcggc ctcaccggat acttgctctt cggagtaact
     5821 gccggaatag tgatcgccgt gatagcgcag gtaagtccac tctgcggtta ggagccgcgg
     5881 atgatttttc agcatgtcgt gaatacatag cgcggcgtga cgccgctcca aaaccttgaa
     5941 tacttcgggg cacagccacc gctcatcgcg gaactcgacg gcaaaacgga gactgcgggg
     6001 ggcggccgcg aggaactcgt ccagccgttc ggcattgaca ttccaacgag gccgaagttg
     6061 gacgagtatc gggccgagcg tctgcttgag acgtttggtg acagtgagaa aattgccgag
     6121 cgttgagcgc ggctccttta gccgcatgaa gtgagtaccg tag
//
LOCUS       DASULZ010000389         3736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_454551, whole genome shotgun sequence.
ACCESSION   DASULZ010000389 DASULZ010000000
VERSION     DASULZ010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454551"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VFY96_08055"
     CDS             complement(<1..214)
                     /locus_tag="VFY96_08055"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HEX5606452.1"
                     /translation="MRGNALSLGIGPIKIPRYLDREQLVTRISQNRFAIAENDRWAEP
                     LEENFSRVLSENLSILLQIDRVAAFPW"
     gene            complement(358..1362)
                     /locus_tag="VFY96_08060"
     CDS             complement(358..1362)
                     /locus_tag="VFY96_08060"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX5606453.1"
                     /translation="MRKKISPAMIGAFVVGAVALIVAAFVIFGSGRLFRQTRDFVLYF
                     DNSVNGLRVGAPVKVKGVEVGAVKDIRLQLEKSLAVDKIPVIIEIDQEKLTSRGASGD
                     IAGDREGLRRLIVARGLRGQLEMESLVTGLLYVALDFFPGSPLNLVQQGDDYKYPEVP
                     TLPTAFEQAKDAVTQIMNKIEEIDFKGLIASLDQTVDGIRHTVNSPDLEATIRSLRQT
                     MPKLDEAVGNIRILAGTMNENFKTLSSDLQQTSGDTRLALKQATDALKQTEVTMKRAE
                     AAVTNIESITDQDSTVNYELARGLKEVSAAARSLRSLTGYLERNPRALIFGKPAAKEN
                     "
     gene            complement(1359..2144)
                     /locus_tag="VFY96_08065"
     CDS             complement(1359..2144)
                     /locus_tag="VFY96_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5606454.1"
                     /translation="MTGEINITNGSKPHITVRDLTMAFGSFVLMRDLNFTINHGDVFI
                     IMGGSGCGKSTLLRHLIGLIEPSRGDILYSDENFTKAEPEQRQELLRRMGVLFQTGGL
                     WSSMTLSENIGMLLEEYTDLSPAEIREVASLKLALVGLKGFEDYYPSQISGGMQKRAG
                     LARAMALDPEVLFFDEPSAGLDPISSRLLDDLILELRDSLGATVVVVTHELASIFTIG
                     NNSVFLDPDAKTMTASGDPKELLEHCPDPKVRAFLRRGEKEES"
     gene            complement(2144..3274)
                     /locus_tag="VFY96_08070"
     CDS             complement(2144..3274)
                     /locus_tag="VFY96_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5606455.1"
                     /translation="MAENSQVSFTFAADSTLNVQLRGAWRLRSGVPPATLVERELETR
                     RARSVAFNADELGAWDSSVLIFLVQISELCRQRKISMDRAGLPGGVQKLLALAEAVPE
                     RKGARREVSEASILERIGSTTIGVAGSLTEMLAFLGEMTITFVRLFGMQARYRASDLL
                     LLIQQCGAQALPIVTLISFLVGVILAFVGAVQLKQFGAQIYVADLVGIAMMREMGAMM
                     TGIIMAGRTGAAFAAQLGTMKVTQEVDALTTAGFSPLEFLVLPRVIALILMMPLLCLY
                     SDFVGVLGGAAIGVGMLDLSGTTYFNETAAAITLTDIFGGVFKSAVYGVLIALSGCLR
                     GLQCGNSSSAVGDAATSAVVTGIVAIVVACGVFAVVFYVLAI"
     gene            complement(3305..>3736)
                     /locus_tag="VFY96_08075"
     CDS             complement(3305..>3736)
                     /locus_tag="VFY96_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013324999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HEX5606456.1"
                     /translation="SISSEHQCLAQNALLVQSLIKLYSCYGNDVKVECPTGSGHGMNL
                     WEVADEIDRRLCQIFLRGDQGTRPVYGGSEKFQRDPYWRDPILFYEYFHGDNGAGIGA
                     SHQTGWTGLVAWSIKSRAVLDPAEVLQNGFDSVAMKIVRSY"
BASE COUNT          852 a         1098 c          994 g          792 t
ORIGIN      
        1 cccacgggaa agccgccact ctgtcgatct gaagcaaaat tgataaattt tccgagagca
       61 ctctcgaaaa gttttcttcc agcggctccg cccaacgatc attctcggca atggcaaagc
      121 ggttttggga aattcgcgta actagttgct cgcgatcgag ataacgggga attttgatgg
      181 gaccaatccc gagagaaagc gcgttaccac gcatgctgtt ctcaggcctc tgctccgtcc
      241 gcggcagaga agtcaaagca aaaaaccttg acgggtccgg tttcgcctcg agagatagac
      301 aactcgccgt ggtaaaacat gcgaccaata atatccgcca tagtttattg caagacatca
      361 gttttccttt gccgccggtt tcccaaagat aagcgctctg ggattgcgtt ccaggtaacc
      421 ggtcaacgat cgcagtgacc gcgccgccgc agagacttcc ttaagccccc tggctagctc
      481 gtaattcacc gtggagtctt gatcggtgat gctctcgata ttggtcaccg ctgcctccgc
      541 tcgtttcatg gttacctcgg tctgcttgag cgcgtccgtc gcttgtttca aggccagacg
      601 cgtgtccccg gaggtctgct gcaggtcgct ggacagggtt ttgaaatttt cgttcatcgt
      661 gcccgcaaga atacgaatat tgcccaccgc ctcatcgagc ttgggcatgg tctgcctcag
      721 tgatcgaata gtcgcctcca ggtcggggga attaaccgtg tgcctgatgc cgtcgacggt
      781 ttgatccagg gaagcgatca ggcccttaaa gtcgatttcc tcgattttgt tcatgatctg
      841 agtgacggca tctttggcct gctcgaaagc ggtcggcaga gtcgggacct ccggatactt
      901 gtaatcgtcg ccttgctgga cgaggtttag agggctgccg ggaaaaaagt ccagcgcgac
      961 gtagagcagg cctgtgacca aactctccat ctccagttgg ccccgcaggc cacgggcaac
     1021 gatcaatcga cgcaaaccct cgcggtcccc tgcgatatcg ccactcgctc ccctcgaggt
     1081 cagcttttcc tgatcgattt cgataatgac gggaatcttg tcgaccgcca ggcttttctc
     1141 gagctgcagc cggatgtcct tcaccgcccc gacctctaca cccttgactt ttaccggagc
     1201 gccgacgcgg agcccgttca ccgaattgtc gaaatagagc acaaagtctc tggtctggcg
     1261 aaacagccgt cccgaaccga aaatcacaaa cgccgcgacg atcaaagcca cggcgcccac
     1321 cacaaatgcg ccgatcatag ccgggctgat ttttttcctc atgattcctc tttttctccg
     1381 cggcgcagga aagcccgaac cttgggatcg gggcaatgtt ccaaaagctc tttgggatcg
     1441 ccgctggcgg tcatggtctt ggcgtcggga tcgagaaaca cactgttgtt gccgatggtg
     1501 aagatactcg ccagctcatg agttacgacg accacggtgg cccccaagct gtcgcgcagt
     1561 tcgagaatga gatcgtccaa taatcgcgag ctgatcggat ctaggccggc cgagggctcg
     1621 tcgaagaaaa gcacctcggg atcgagcgcc atagcgcggg ccaggcctgc tcttttctgc
     1681 ataccgccgc tgatttgcga cgggtagtaa tcttcgaagc ccttgagtcc caccaatgca
     1741 agtttgagcg aggccacttc ccgaatctcg gcggggctca aatcggtgta ctcttccagc
     1801 agcatcccga tgttttccga cagggtcatc gagctccaca gcccgccggt ttggaaaagc
     1861 acgcccatac gccgaagcaa ctcttgtctt tgctccggtt cagctttggt aaagttctcg
     1921 tcgctgtaca agatatcgcc ccgagagggc tcgatgagac cgatcagatg ccggagtaaa
     1981 gtactttttc cgcaaccgct ccctcccatg atgataaaaa cgtcgccatg attgatggta
     2041 aagttaagat cgcgcatgag cacgaagctg ccaaacgcca tcgtgagatc acgcacagtg
     2101 atgtggggtt tgctcccatt ggtgatattt atttctccgg tcatcagatt gcgaggacat
     2161 aaaaaaccac ggcgaagacg ccgcaggcga ccacaatggc gacgatcccc gtcaccaccg
     2221 ccgatgtcgc cgcatctccg actgcggaag agctgttgcc gcactgcaaa cccctgagac
     2281 aaccggaaag cgcaatcagc acgccgtaaa ccgcactttt gaagacgccg ccgaaaatat
     2341 ccgtcagagt aatggcagcg gcagtctcgt taaagtaagt ggtcccggat aaatcgagca
     2401 tgccgactcc gatggcggcg ccacccagca caccgacaaa gtcggaataa aggcacaaca
     2461 atggcatcat cagtatcagc gcgatgacgc ggggaagaac taaaaattcc agcggagaaa
     2521 agcccgctgt ggtgagcgca tcgacctcct gcgtcacctt cattgtgccg agctgggcag
     2581 caaacgccgc tcccgtccgt ccggccatga taatccccgt catcatggcg cccatctcgc
     2641 gcatcatggc gattccgacc agatccgcga catagatttg cgcgccgaac tgcttgagct
     2701 gcaccgcgcc gacaaaagcg aggatcacgc cgaccaaaaa acttataagc gtaacgatcg
     2761 gtagcgcctg ggcgccgcac tgctggatca aaaggagcag atccgaagca cgatagcgcg
     2821 cctgcattcc gaaaaggcgc acgaaagtaa tggtcatttc tccgaggaaa gcgagcatct
     2881 cggtcagcga accggcgacg ccgatggtgg tcgatccgat ccgttcaagt atcgatgcct
     2941 cgctcacttc cctgcgcgcc ccttttctct cgggcacggc ctcggcgaga gcgagtagtt
     3001 tttgtactcc gcccggcaag cctgcgcgat ccatcgagat cttccgctgg cggcagagtt
     3061 cggagatttg caccagaaaa atcaacacac tgctgtccca tgcgccaagc tcgtcggcat
     3121 tgaatgcgac ggatcgcgcg cggcgcgttt ccagctcgcg ctccaccaaa gtggccggag
     3181 gaaccccgct acgcaatcgc caagcgccgc gaagttggac gttcagagta gaatcggctg
     3241 cgaaggtgaa tgatacctga ctgttttctg ccattttgaa aacggttcag ccgcggcggc
     3301 tgccttagta cgagcgaaca atcttcatgg cgacgctgtc aaagccgttc tggagcacct
     3361 cggcgggatc caagaccgct cgcgatttga ttgaccaggc aactaatcct gtccaacccg
     3421 tctgatgact ggcgccgatt ccggcgccgt tatcgccgtg aaagtattcg tagaacagaa
     3481 tcgggtcgcg ccaataaggg tcacgctgaa acttttctga tccaccgtac accggacgcg
     3541 tgccctgatc gccgcgcaaa aagatttggc atagacgtcg atcgatttca tccgcgactt
     3601 cccataagtt catcccatgg ccggacccgg taggacattc aactttgaca tcgttaccat
     3661 agcaggagta gagctttatg agcgattgga ccaatagtgc attttgcgcg agacattgat
     3721 gttcactcga aatgct
//
LOCUS       DASULZ010000390         3697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1164945, whole genome shotgun sequence.
ACCESSION   DASULZ010000390 DASULZ010000000
VERSION     DASULZ010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164945"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VFY96_08080"
     CDS             <1..843
                     /locus_tag="VFY96_08080"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5606457.1"
                     /translation="VTILTGVPDVGPGYYTVSQQIVSEVLGLPPEKVGIAFRDTDSLP
                     FDPGTGGSKQTNTSAHAVHQSAREVREKLISLAARELGCKPEEIRQDGAKLIAPSKKS
                     TTTEAMIKLAVKENGAPVFHLTNFVPKDMPKVTGFAAQIAEVEVDATTGGVKVLNLTT
                     AHDTGTVLNHLTLTGQIDGGVVTGLGFALMEENPMTDGKITTLNLGESKLPSIADVPP
                     LKTVLVESPTGPTPFGGKAIAENPNVPTAAAIANAVADASGVRIFDLPLTSEKIYWAL
                     NGKK"
     gene            1030..2166
                     /locus_tag="VFY96_08085"
     CDS             1030..2166
                     /locus_tag="VFY96_08085"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5606458.1"
                     /translation="MIRFALLFLITIGLLFPIARPSVAAQVNTSRQAEWEQTVRAAEK
                     EGQVTVSIGGYGAIIDSGVFQKAFPKIKVTSMTGAGTDLTARIAAERRAGKYLVDVYN
                     GGGNSLFQVLYRGKMLDPIKPALLLPEVVDPTKWWEGKQKYADKEGQYILVYEGNVAA
                     GAGAGYNTQLLDPSEYRSYWDFLNPKLKGKIMSTDIRKLRGAGIPWQFLYYYPDLGPK
                     FLRRLFGEMDVTMVADLRQAVDWLGTGKFALCLPVQGGTIFKAKNQGLPVDQFESYHF
                     KEGVNLSSAFGSLALLNRAPHPNAAKVFINWLLSREGQTLFQKVISVPGDARNSRRID
                     VPKDHIPPDEQRRDRMPYFDTDDSDTKDLTPVMKLLDEIFAAKK"
     gene            2198..3334
                     /locus_tag="VFY96_08090"
     CDS             2198..3334
                     /locus_tag="VFY96_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5606459.1"
                     /translation="MKTLRNVCLVLFALILPLRGSGIAAEAKPGWQTTWEKTIEAAKK
                     EGHLNFYVGRYGSEPLLNEFRKEFPWLKLVTVNGSGNSLGTRIIAEIRAGHVLADVFS
                     GGANTNYEVLYEGKALDSFKSTLILPDVIDETKWFEGRHRYTDPEQRYIFVYVANPSS
                     SGFYYNTTLVNPKEFKSYWDLVNPKWKGKYVSQDPLSTGLGASLQFYYYHPELGADYI
                     KKLFGDMQPVFGRDRRQITDWLAQGRYALCVGCRDTNRAKNQGLPVDELDNVDWKEGV
                     QLTSGGGSMSVIKGGPNPNAAKVFINWFLSRRGQLALQKYDDLYGEDAPNSRRTDIPK
                     DMLVPVMRLKTGTKYFDVSDPKYADMTPMFNVVKEALKGREGKQ"
BASE COUNT          919 a          961 c         1036 g          781 t
ORIGIN      
        1 gtaacgattc tcaccggcgt tccggatgtg gggccgggat attacaccgt cagtcagcaa
       61 atcgtctccg aagtgctcgg gttgccgccg gagaaagtcg gcatcgcatt cagagacact
      121 gacagtttgc ccttcgatcc gggcaccggc ggcagcaagc agaccaacac ttccgcacac
      181 gcggtgcacc aatccgcgcg cgaagtccga gaaaaattga tcagcttggc cgcacgggaa
      241 ctaggctgca agccggaaga gattcgccag gatggcgcga aactaatcgc gcccagcaag
      301 aaatccacaa ccacggaggc aatgatcaaa cttgccgtca aggaaaacgg cgctccagtg
      361 tttcatctca cgaactttgt tccgaaagac atgcccaaag taacaggctt tgccgcgcag
      421 atcgccgagg tcgaagtcga tgcgacgacg ggcggagtca aggtgctcaa tttaactacg
      481 gcgcacgata cgggaaccgt gctcaatcat ttaacgctca cgggacaaat cgatggcggc
      541 gtcgtcaccg gtctcggctt cgcgttgatg gaagaaaacc cgatgaccga cggcaagatt
      601 acaaccttga acttgggtga atcgaagctt cccagcatag ctgatgtccc gccattaaaa
      661 accgtgctgg tcgaaagccc gacgggaccg acgccgttcg gcggtaaggc gattgcggaa
      721 aaccccaacg tgccgacggc ggcagcgatt gcgaatgcag tagcggacgc ctccggcgtg
      781 cggattttcg atctgcctct gacatcggaa aaaatttatt gggcgctgaa cggaaaaaaa
      841 tagcggcgcg gaaatgcgga cgccagttga acaacgatat cgtaacggtg tgtaatcctg
      901 agccgatagg cgaaggatct cgtcattggc cattaacacg agatgcttcg ctgtcgctca
      961 gcatgacagg tgcgagcttc gcgtgaagca gtgaacgccc agcggacgag taaatatgtg
     1021 caacttgaga tgattagatt tgcgctgctg tttctaatta cgatcggttt gctcttcccg
     1081 attgctcgac cgagcgttgc ggcgcaggtc aatacgtccc gccaagctga atgggaacaa
     1141 acggtccggg ctgcggaaaa agaaggccaa gtgacagtct cgatcggcgg ctatggggcg
     1201 atcatcgact ccggtgtctt tcagaaagca tttccgaaga tcaaagttac gagcatgacc
     1261 ggagcgggaa ccgatctcac cgcgcgtatc gccgcggagc gacgagccgg caaatatctc
     1321 gttgacgtct ataatggtgg tggcaactcg ctgtttcaag ttctttaccg cggaaagatg
     1381 ctcgatccga tcaaaccggc tctcttactg ccggaagtcg ttgacccaac gaaatggtgg
     1441 gagggtaaac aaaaatacgc cgacaaagaa ggtcaatata ttctcgttta cgaaggcaat
     1501 gtggcggccg gtgccggcgc gggttataac actcagttgc tcgatcctag cgagtacagg
     1561 agctattggg atttcctcaa tcccaagctc aagggcaaga tcatgtccac ggatattaga
     1621 aagctgcgcg gcgcgggaat tccctggcag tttctatatt actaccccga tcttggtccc
     1681 aaatttcttc gacggctttt cggtgaaatg gatgtgacga tggtcgccga tttgcgccaa
     1741 gcggtggatt ggctcggcac cggcaaattt gctctatgcc tgccggtgca gggcgggacg
     1801 atcttcaagg cgaaaaatca gggactgccg gtggatcaat tcgagtcgta tcatttcaaa
     1861 gagggtgtga acctttcatc ggcgttcggc tccctcgccc tgttgaatcg cgcgccgcac
     1921 ccaaatgccg ccaaggtctt catcaactgg ttactgtcgc gtgaaggcca aacgctattc
     1981 caaaaggtga tctccgtgcc gggagatgcg cggaactcgc gcagaatcga tgtgccgaag
     2041 gatcacatcc cgcccgacga gcagcggcgc gacagaatgc cctatttcga taccgatgat
     2101 tcggatacga aggacctaac gcctgtaatg aagctgctgg atgaaatatt cgccgcaaaa
     2161 aaataaatca cttgaaacgg cagaaggaga attgaagatg aaaacgttgc gcaacgtttg
     2221 tttggttttg tttgcgctga ttctcccact gcgaggatca ggaattgcgg cggaggcgaa
     2281 gcccggatgg caaacgacct gggaaaaaac catcgaggct gccaagaaag aaggacacct
     2341 gaatttctat gtcggccgct acggcagcga gccgctgctc aacgagtttc gtaaagaatt
     2401 tccctggctg aaacttgtca cggtgaacgg atcaggcaac tccttgggca cgaggatcat
     2461 cgcagaaatt cgcgccggtc acgtcctggc cgatgttttc agcggcggtg ccaatacgaa
     2521 ttacgaggtt ctctatgaag ggaaggcgct cgattcgttt aaatctactc tgatcctgcc
     2581 cgatgtcatc gacgagacta aatggttcga gggacggcat cggtacaccg atccggagca
     2641 aagatacatc tttgtttacg tcgcaaaccc ttcatcttcg gggttctatt acaacaccac
     2701 tctggtaaac ccgaaagagt ttaagtcgta ttgggacttg gtcaatccca aatggaaggg
     2761 aaaatacgtt tctcaggacc cgctcagtac cggcctcggc gcttccctgc aattttacta
     2821 ttatcaccct gagctcggcg ccgattacat caaaaaactg ttcggtgata tgcagccggt
     2881 attcggccgt gaccggcgac agatcacgga ttggctggca caaggccgtt acgccctctg
     2941 cgtcggttgc cgcgatacca accgggcaaa aaatcaggga ttgcccgtgg acgagctgga
     3001 caacgtcgat tggaaagaag gagtgcagct cacttccggc ggcggttcga tgagcgttat
     3061 caaaggcggt ccgaatccca acgcggcgaa ggtgttcatc aattggtttc tctcgcgccg
     3121 cggccagctc gcgttgcaaa aatacgatga cctctacggc gaggatgcgc cgaactcccg
     3181 tcgtacggat ataccaaagg atatgctggt gccggtcatg cgattaaaaa ccgggacgaa
     3241 atattttgac gtttccgatc ccaagtacgc ggatatgacg ccgatgttca atgtggtgaa
     3301 ggaggcgctg aagggaagag aggggaagca gtgaggaacg gcaaagagct aatagagaag
     3361 ccattcgtcc gtcatgtcgt cacacgccgg catccaggtg ttcacggttt cagcgcggaa
     3421 gacgctggat tccagcagcc gccggaatga tgaataatgt gtctcgcgcc actccagccg
     3481 tgttcggctg ctcgatcagc gtcattcccg catgctttta agcgggaatc caggagacgg
     3541 aaatggtcga acaatgatga gcatcgtttc catccatcct cctactgtga cttcgatggg
     3601 ttatcatttt ttccattcag cgcagagtaa atcttctccg aggtgatcgg caaatcatat
     3661 aacctcacgc cgaccgcgtc ttcgatcgca ttggcga
//
LOCUS       DASULZ010000391         2138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_860685, whole genome shotgun sequence.
ACCESSION   DASULZ010000391 DASULZ010000000
VERSION     DASULZ010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860685"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="VFY96_08095"
     CDS             complement(<1..948)
                     /locus_tag="VFY96_08095"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5606460.1"
                     /translation="MLTAEENEYLTRVGPGTPAGELLRRYWHPVAIAADLTPESPTKF
                     VRILGEDLVLFVTPEGEPGLLHDRCAHRGASLSYGRVEARGIACAYHGWLYDTKGNCL
                     ETPAEPAGSNFCLTVKQKAYPVQKLAGLYWAYLGPQPAPIIPKYDVWARTDGWHWLRA
                     LPQLDCNWLQAMENSVDTAHLHILHQELVTDGFKVENTTRGTIDNLVKYEMEEFDHGI
                     IKRRHFRDGKIEEHPLLFPTVLRQANRTQIRVPIDDTHTWIVYVHFVPDDAEPRANKD
                     EVPVNYRKPFKNPLGERHPFTKFRLDEVDAQDFMAWETQG"
     gene            complement(999..>2138)
                     /locus_tag="VFY96_08100"
     CDS             complement(999..>2138)
                     /locus_tag="VFY96_08100"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5606461.1"
                     /translation="TQILPPISALPYIKITAAADTRPDALEKFRAVYLGETYTSVQAM
                     CEEADIDAVYVATPNPLHAEHTITAAGSGKHIIVEKPMAMSLAECDAMNEAAEKNGVK
                     LLCGHTHSFDPPIRKMREIVKCGGLGKLCMINGWNYNEFMYRPRMRHELAMSRGVVLN
                     QGPHQVDIVRLIGGGMVRSVRAMTGIWDKAREWEGSYSCFLEFEDGTPATLVYSGYGF
                     FDTAELFDWVGEGGQYRAPQTNLNVRKKLREVRTTEEEEQLKEGMRFGGQREGEYSHV
                     WGGERKQPFFGLIVVSCEKGDIRQTPDGLKVYGETEQREIRLPAANRGREAEVEELYN
                     AVVHNRPVFHDGRWGAATLEVCLAMLESAAQRKEIFLSHQVPSPE"
BASE COUNT          423 a          666 c          563 g          486 t
ORIGIN      
        1 tccttgagtt tcccaagcca taaaatcttg cgcatcgact tcgtcgaggc ggaatttggt
       61 gaatggatgg cgctcaccga gtggattttt gaacggcttg cggtaattca ccggtacttc
      121 gtccttgttc gcgcgcggct cagcgtcatc cggcacgaag tgaacgtata cgatccacgt
      181 atgggtatca tcgatcggca cgcgaatttg cgtgcgattg gcctggcgca aaacggtggg
      241 aaatagcaac ggatgctctt cgatcttgcc gtctcggaaa tggcgccgct tgatgatgcc
      301 gtggtcgaac tcctccattt catacttgac caggttgtcg atcgtaccgc gagtcgtgtt
      361 ttcgactttg aagccatcgg ttacaagctc ctgatggagg atatgcaggt gcgccgtgtc
      421 gacggaattt tccatcgcct gcagccaatt gcagtcgagc tgcggcaaag cgcgcagcca
      481 atgccagccg tccgttcgtg cccagacatc gtacttcgga ataatgggcg ccggctgcgg
      541 tcccagatac gcccaataca gacccgccaa tttttgcact ggataagctt tttgcttcac
      601 cgtcaagcaa aagttacttc cggcgggctc cgccggagtt tccaggcaat tgcccttcgt
      661 gtcataaagc caaccgtgat acgcgcacgc gataccgcga gcttcgactc gaccgtaaga
      721 caatgatgcg ccgcgatgcg cgcaccgatc gtggagcaga ccaggctcgc cttcgggcgt
      781 cacaaacaat actagatcct cgccgaggat gcggacgaac ttcgttggac tttccggcgt
      841 caaatcggcg gcgatcgcga cgggatgcca gtagcgacgc aacagttcgc ccgccggcgt
      901 tcccggcccg actcgcgtca aatattcgtt ctcttcagcg gtaagcatcg atcaaccctc
      961 cggaataaac ttcaaacccg agactcgaaa ccaaacctct actccgggct cggcacctga
     1021 tgcgaaagaa aaatctcctt tcgctgcgcc gctgattcga gcatcgccaa gcaaacttcg
     1081 agcgtcgccg cgccccagcg gccatcgtga aacaccggcc ggttatgaac gacggcgttg
     1141 tagagctcct cgacctcggc ttcgcgcccg cggttagcag cgggcaaccg gatctcccgc
     1201 tgttccgttt caccgtaaac ctttaaaccg tccggcgtct gacgaatatc acctttttcg
     1261 cagctcacaa ctattaaccc aaagaacggc tgcttccgct cgccacccca cacgtggctg
     1321 tattcgccct cacgctgccc gccgaagcgc atcccttcct tcagctgttc ttcctcttcc
     1381 gtcgtccgta cctcgcgcaa tttttttcgg acattgagat tggtttgcgg tgcgcggtac
     1441 tgtccgccct cgccaaccca gtcgaacaat tccgcggtgt caaaaaaccc gtagccgcta
     1501 tagaccagcg tcgcgggcgt gccgtcttcg aattcaagaa agcagctgta actaccttcc
     1561 cactcgcgcg ctttgtccca aatccccgtc atcgcgcgta cgctgcgcac catgccgccg
     1621 ccgatcaggc gcacgatgtc gacctggtgc ggtccttggt tgagcacgac cccgcggctc
     1681 atcgccagct cgtggcgcat gcgcggccgg tacatgaatt cgttgtagtt ccagccgttg
     1741 atcatacaga gcttgcccag cccgccgcac ttgacgatct cgcgcatttt acgaatcggc
     1801 ggatcgaagc tgtgcgtatg gccgcagaga agcttcacac catttttttc cgctgcctcg
     1861 ttcatggcgt cgcattccgc cagcgacatc gccatgggtt tttcgacgat aatgtgtttt
     1921 ccactgcccg ccgcggtgat cgtgtgctcg gcgtgcaacg gattgggtgt ggcgacataa
     1981 actgcgtcaa tgtccgcctc ttcgcacatc gcttgaacgc tcgtgtaggt ttcgcccagg
     2041 taaactgcac ggaatttttc cagagcgtca ggacgggtat ccgccgctgc tgtaatttta
     2101 atgtacggca acgccgagat cggtggcaga atctgcgt
//
LOCUS       DASULZ010000392         4871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_251389, whole genome shotgun sequence.
ACCESSION   DASULZ010000392 DASULZ010000000
VERSION     DASULZ010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251389"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1798)
                     /locus_tag="VFY96_08105"
     CDS             complement(<1..1798)
                     /locus_tag="VFY96_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HEX5606462.1"
                     /translation="MESQRADVTEQKRLNEAREKGIPWKKWGPYLSERQWGTVREDYS
                     ENGDAWNYFSHDQARSRAYRWGEDGLGGISDDKQRLCFALSLWNERDAILKERLFGLT
                     NSEGNHGEDVKEYYFYLDSTPTHSYMKYLYKYPQREYPYADLVETNRRRSRRESEYEL
                     IDTGIFNDDRYFDVFVEYAKAEPEDILVRITVHNRGPEGARLRVLPTLWFRNTWSWGE
                     NDVPKPRLCEIRPDAIQASHDELGELWLYCDGAPELLFTENENNAKRLWGQPNASPYV
                     KDAFHEYLIAGRGEAVNPAKTGTKAAAHYRLAVAAGGSQTIRLRLSASGIKNPFDGFD
                     SMIKTRVADADEFYDRIAPNALAEDERRVHRQALAGMLWSKQYYYFDLEHWLREHKSH
                     PLLESARRDVRNTEWFHMLNADVISMPDKWEYPWYAAWDLAFHTISLSLVDFDFAKEQ
                     LLLMLRSLYFHPSGQIPAYEWNFSDVNPPVHAWATLYLYKYERTLGRADLRFLERSFQ
                     GLTLNFNWWVNRKDPAGRNVFAGGFLGLDNIGVFDRSAQLPTGGTLEQADGTAWMAFY
                     CQNMLEMALILTEYDPMYEDVAFKFIQHFMWIA"
     gene            complement(1876..3249)
                     /gene="zwf"
                     /locus_tag="VFY96_08110"
     CDS             complement(1876..3249)
                     /gene="zwf"
                     /locus_tag="VFY96_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX5606463.1"
                     /translation="MNQQAADALVFFGASGDLAYKKIFPALQAMVRAGRLDIPVIGVA
                     KSGWTRDQLIARAKASVTDHGGFDATAFARLTELLHYVDGDYNDRTTFAQLRAELGEA
                     ERPTHYLAIPPTLFEIVIRQLGETGCAKGARVIIEKPFGHDLASAQELNSVVHRIFDE
                     RNVFRIDHYLGKNTVQNLLFFRFANSFLEPLWNRQFVESVQITMAESFGIEGRGQFYD
                     GTGALRDVVQNHLLQVLTNVAMEPPPGLDVEMLRDEKAKVLRGIRALQSADVVRGQFR
                     GYRDEPGVSPNSQTETFVALKLEINSWRWKGVPFFIRAGKNLPVTVTDVIVKLRQPPA
                     IFSGVPLPSNHMRFRVTPNLQIAIGALVKKTGERLEGDQVELLISEESDPTELGAYGE
                     LLHDAIEGDSARFARQDYVEESWRIVDPALTGAGPLYSYEPGSWGPAEADRLIDAIGG
                     WNNPTAS"
     gene            complement(3415..>4871)
                     /locus_tag="VFY96_08115"
     CDS             complement(3415..>4871)
                     /locus_tag="VFY96_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606464.1"
                     /translation="ILSVLAIIFFKGFSEAIGLAAAAALPYLVLNLVVLGRGAWEIMA
                     HPGLLAEWHSALAAKGDFPLLIAGALLVFPKLALGLSGFETGVTVMPLIDGGEEDREH
                     SPRAGAAPMGRVANTKKLLITAAIIMSGMLILSSLVTALLIDRADYREGGKASGRAIA
                     FLAHRYLGPSFGTVYDMSTILILGLAGASAMAGLLHLIPRYLPRYGMAPRWAALSRPL
                     ILVLFIIDIVITLIFGASVEAQSGAYATGVLVLILSAAFATTLALWRERRWALACYMG
                     VLLLVFAYTLADNCLERPDGLIIGTIFTLLLILTCALSRSLRSVEFRIPAGYFTDAES
                     SRIGPELRGKKVHLVPISSSSAEARRKKSEEIAKYYGVRGPFLFLHVNLLDNRSEFSS
                     PLEVSLRKDGKNYVGEVFGAVAIANTIAYVSEAIDPISIFIGLTRRNLMAQAVRYLLF
                     GEGETGLIVYTILLRYWDWTPEQDVRPLIFLMSD"
BASE COUNT         1097 a         1421 c         1340 g         1013 t
ORIGIN      
        1 aggcgatcca catgaaatgt tggatgaact tgaaggcgac atcttcgtac atcggatcgt
       61 attcggtcaa aatgagggcc atttccagca tgttctggca gtaaaacgcc atccacgcag
      121 tcccgtccgc ctgttccagc gtaccgccgg tcggcagctg cgcgctgcgg tcgaacacac
      181 cgatattgtc gagcccaaga aaaccgcccg cgaaaacgtt ccgcccagcg ggatccttgc
      241 ggttgaccca ccagttgaag ttcaacgtca gtccctgaaa cgaacgttcc agaaaacgga
      301 ggtcggcccg gcccagggtc cgctcgtact tatacagata aagcgtggcc caggcgtgta
      361 ccggcggatt gacatcgctg aaattccact cgtaggcggg aatctgcccg ctcgggtgga
      421 agtacaggct ccgcaacatc agcagcaatt gttccttagc gaagtcgaaa tctaccagcg
      481 atagcgatat cgtatgaaag gccaggtccc aggccgcgta ccacgggtac tcccacttgt
      541 ccggcatgga gatcacgtcg gcattgagca tgtggaacca ttcagtgttg cgcacgtcgc
      601 gtcgcgcgga ctcgagcagc ggatggctct tgtgctcgcg caaccagtgc tccaggtcga
      661 agtagtagta ctgcttggac cagagcattc cggccagagc ctggcggtgc acccggcgct
      721 catcttcggc aagggcgttc ggcgcaattc gatcgtagaa ttcgtcggcg tcggcgactc
      781 gggttttaat catcgaatcg aaaccgtcga acggattttt tataccgctc gcactcagcc
      841 ggagacgaat ggtttgactt ccgcccgcgg cgactgcgag ccgataatgg gccgcggctt
      901 tggttccggt cttcgcggga ttcaccgctt cgcctcgtcc cgcgatgagg tattcgtgaa
      961 aagcatcctt gacgtacgga gaagcattgg gctggcccca tagacgtttg gcattgttct
     1021 cattctcggt gaagagcaac tccggcgcgc cgtcgcaata gagccacaac tcgcccagct
     1081 cgtcatgtga cgcttgaatt gcgtcaggtc ttatttcaca tagtcgcggc ttcggtacgt
     1141 cgttctcacc ccaactccag gtgttgcgga accatagtgt cggcaagacc cgcaaccggg
     1201 cgccctcggg cccacgattg tgcaccgtaa tacgaacaag aatatcttcc ggctccgcct
     1261 tggcgtattc gacaaacacg tcaaagtacc gatcgtcatt aaatatgccg gtgtcgatga
     1321 gttcgtactc ggattccctc cgtgaacgtc tccgattggt ctcgacgaga tccgcatacg
     1381 gatactcgcg ctgcgggtac ttgtacaaat atttcatgta cgagtgcgtc ggcgtgctgt
     1441 cgagatagaa atagtattcc ttgacatcct cgccgtgatt tccctcactg ttggtgagcc
     1501 cgaagaggcg ctctttcaaa atcgcgtcac gttcgttcca caagctcaat gcaaagcaga
     1561 gccgctgctt gtcgtcggag attccaccga gaccatcctc gccccaacga taagcgcgcg
     1621 agcgcgcctg atcatggctg aaataattcc aggcatcgcc gttttcgctg tagtcttcgc
     1681 ggaccgtgcc ccattgccgc tcgctcaagt agggacccca ttttttccat gggatcccct
     1741 tctcgcgcgc ctcgttcaaa cgcttttgct cggtgacatc tgctcgctgc gattccataa
     1801 cgtttcctta gatcctattt gcttttataa aaagttcgtc cctcgttggc ctggcgttgt
     1861 agtgagttta gctaatcaac tcgctgtcgg gttgttccag ccgccgatcg catcgattag
     1921 ccggtcggct tcggcgggac cccagcttcc cggttcgtaa ctgtagagcg ggccggcgcc
     1981 ggtcaaagcc ggatcgacga tacgccagga ctcttcgaca tagtcttggc gggcaaaacg
     2041 agcggaatcc ccttcgatcg cgtcatgcaa aagctctccg taggcgccca actcggtggg
     2101 atccgattcc tcgctgatca gtagctcaac ctgatctcct tcgagccgct cgcccgtctt
     2161 tttcaccaaa gcgccgatcg cgatctgcag attcggcgtg acgcgaaacc gcatatgatt
     2221 ggatggaaga gggactcctg aaaaaatggc cggcggctgg cgcagcttga caataacatc
     2281 tgtcaccgtg actggaagat tcttcccggc ccggataaaa aacggcacac ccttccagcg
     2341 ccacgaattg atttcaagtt taagcgcgac aaaagtctcg gtttgcgagt tcgggcttac
     2401 ccccggctcg tcacggtagc cgcgaaactg accgcgcacg acatccgccg actgcaaggc
     2461 tcgaattcct ctcagcactt tcgccttctc atcgcgcaac atttcgacat ccagtccggg
     2521 cggcggctcc atcgcaacgt tggtcagcac ttgcagaaga tggttctgca ccacgtcgcg
     2581 cagcgcgccg gtgccatcgt aaaattgccc gcggccctcg atcccgaaac tttccgccat
     2641 cgtgatctgc acgctctcga cgaactggcg gttccacaac ggctcgagga aggaatttgc
     2701 gaaacggaaa aacagcagat tttgaaccgt gtttttaccc aaatagtggt cgatacgaaa
     2761 tacatttcgt tcgtcaaaaa tgcggtgcac gacggaattt aactcctgcg cggatgcgag
     2821 gtcgtgtccg aacggctttt caatgatgac acgcgcgcct ttggcacaac ccgtttcgcc
     2881 aagctggcga atgacgatct cgaagagagt cggtggaata gcgagataat gcgtcggccg
     2941 ttccgcctct ccgagctctg ccctgagctg tgcgaaagtc gtccggtcat tgtaatcacc
     3001 gtcgacgtaa tgaagcagct cggtcaaacg agcaaacgca gtcgcatcaa agccgccgtg
     3061 gtctgtaacg cttgccttgg ctcgtgcgat caactgatcg cgtgtccagc cggacttggc
     3121 gacgccgatc accggaatat cgagccggcc cgctcggacc atggcctgaa gcgcgggaaa
     3181 aattttctta taggcgagat cgccactggc accgaaaaat accagtgcat cggccgcttg
     3241 ttggttcatc gttccgcctc gattgccctg tcgcgatttt ctcaggatca aaaaatctgc
     3301 agcgcatcta gcattatttc atttgacgta ggtttgccag acgagcccgt gatcaagcac
     3361 ccggagtcat cgcgataata tgacgtggag aacggatccg atcttcgact gagcttagtc
     3421 ggacatcaaa aagatgaggg gacggacgtc ttgctccggc gtccaatccc agtagcgtag
     3481 caggatggtg taaacaatca gcccggtctc tccctcaccg aacaggaggt agcggacggc
     3541 ttgggccatg agattgcgcc gggtgagtcc gatgaaaatc gatatgggat cgatcgcctc
     3601 gctgacatac gcaatggtat tggcaatggc aaccgcacca aacacttctc cgacatagtt
     3661 cttgccgtct tttcgtaggc ttacctcgag tggcgaagaa aactcgctgc ggttgtcgag
     3721 caggttcaca tgaaggaaca gaaaaggacc gcgcacaccg taatatttag cgatctcctc
     3781 gcttttcttg cggcgcgcct ccgctgaaga actagaaatg ggaaccagat gaactttttt
     3841 gccccgcaac tccggcccaa tgcgcgagct ctcggcatcg gtgaagtatc ccgctggaat
     3901 gcggaattcc accgaacgca gcgatcggct gagggcacag gtcaggatca ggagcagcgt
     3961 gaaaatggtg ccgatgatca ggccatccgg gcgctccaga caattgtctg ccagggtgta
     4021 agcgaacacc aacaagagaa ctcccatata acaagctaaa gcccagcggc gctcgcgcca
     4081 cagcgcaagt gtagtcgcga aggcggctga caaaatcagt accaggacac ccgtagcata
     4141 ggcgccgctc tgggcttcca cggatgcgcc aaagatcagc gtgatgacaa tgtcgatgat
     4201 aaaaagaacg aggatcagcg gtcgcgatag cgcagcccag cgcggggcca tcccgtagcg
     4261 cggcagatag cgagggatca ggtggaggag cccagccatc gccgacgcgc cggcgagacc
     4321 gagaatgagg atggttgaca tgtcataaac tgtgccaaac gaagggccaa gataacggtg
     4381 cgccaaaaac gcgatggcgc ggccgctcgc cttacctcct tctcggtagt ccgcacgatc
     4441 gatcagcaga gcggtgacaa ggctcgaaag aatcagcatg ccactcataa taatcgctgc
     4501 ggtaatcaga agctttttgg tattggctac acgacccatc ggcgcggcgc ccgcgcgcgg
     4561 gctgtgttcg cgatcttctt cgccgccgtc aatcaggggc ataacggtca cgccagtctc
     4621 gaatccgctc agtcccaagg cgagcttcgg gaataccagc aaggcaccgg cgatcagcag
     4681 cgggaaatcg cccttcgccg cgagcgcgga atgccattcg gcaagtaggc ccgggtgagc
     4741 catgatctcc caggcgccgc gccctaggac gaccagattg agaacgaggt aaggcagagc
     4801 ggccgcagcc gccaagccga tcgcttcact gaagccctta aaaaaaataa tcgctaggac
     4861 actgagaatc g
//
LOCUS       DASULZ010000393         2790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1468878, whole genome shotgun sequence.
ACCESSION   DASULZ010000393 DASULZ010000000
VERSION     DASULZ010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468878"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1282)
                     /locus_tag="VFY96_08120"
     CDS             complement(38..1282)
                     /locus_tag="VFY96_08120"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5606465.1"
                     /translation="MADYIKEHYDFPLAKKSENKPLKGVRVIDAGNMVAAPFATVLLA
                     DFGADVIKIEHPKYGDGQRKLEPIKDGIPLWWKSVARNKRCITLDLGKPEGAALFKQL
                     IKGKDVIVENYRPGTFEKWGIGPDVIRGIDPRLILLRISGFGQTGPYKDRAGFGRVAE
                     AMSGLTNLIGEPDGAPMSPGYPLGDLISGIFGSLSVMIALYHRDIRGGEGQVIDLALF
                     EAVFRFLDFDPIQYDQMKTVHMRTGNRVAYVAPSSMFRTKEGKYLTLAASTQSVWLRL
                     AEAIGRKDLTTDPKFIDNPARVVNSVEINGIVGDWIGKHTRQEVIEQFDKFGVAYSAV
                     FDMEDAFRDIQYRAREAMVRVPDPDLGEAIVQNVVPKFSATPGSVDFLGRPMGAHNEE
                     IFCGELGLSKEKLAELKTAGII"
     gene            1568..2347
                     /locus_tag="VFY96_08125"
     CDS             1568..2347
                     /locus_tag="VFY96_08125"
                     /inference="COORDINATES: protein motif:HMM:NF004868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HEX5606466.1"
                     /translation="MAEQPRIVTGLGKAELHRILVRGYDLNKELVGKISFTDMTSLIL
                     RGRLPTGNEARMLDALLVILVEHGMVSHVIAARLIYHCAPEAMQAAVAAALCGAGSVH
                     LGSSEWSAKMLIEALPRDTQNPDLAAIAASIYDDYSKRKQRMPGIGHRTHAEGDPRAD
                     TLFGIAKETGVYGRYCDLLQSLSKVATDRRKRLIPVNVTGAIGAIALDMGFPWQITKA
                     FALIGRTLGAIGHIAEEIQNPMATQIDAAVKQAIVYEPVRT"
     gene            2503..>2790
                     /locus_tag="VFY96_08130"
     CDS             2503..>2790
                     /locus_tag="VFY96_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5606467.1"
                     /translation="MGYQNILTERVDKIAVITMNRPEKLNALSYELACEIDEELTKIE
                     SDDEVRAVILTGAGPRAFSAGGDIMQIVKSTPEEMAARTETRREANWHLATF"
BASE COUNT          629 a          803 c          755 g          603 t
ORIGIN      
        1 ggggatcgag gggatttatg aagcaagttt ttattaacta aataatccct gcggttttca
       61 gctctgccaa tttttctttc gacaatccca gctcgccaca aaagatttcc tcgttgtgtg
      121 cgcccatggg tcggccgaga aaatccaccg agcccggcgt tgcggaaaac ttgggcacga
      181 cgttttgcac gatcgcctca cccaaatccg ggtcgggcac gcgcaccatc gcttcacgag
      241 cacggtattg aatatctcgg aacgcatctt ccatatcgaa cacggccgaa taagcgacgc
      301 cgaatttatc gaactgctcg atcacttcct gccgtgtgtg cttgccgatc caatctccga
      361 caatgccgtt aatttcgacc gagttcacca cgcgcgctgg attgtcgatg aacttgggat
      421 cggtggtcag atccttccgt ccgatcgctt cagccaatcg caaccagacg ctctgcgtgc
      481 tcgcagccag ggtcaaatac tttccctcct tggttctaaa catcgagctc ggcgccacgt
      541 aggcgacgcg gttgccggtg cgcatgtgca cagtcttcat ctgatcgtac tgaatcggat
      601 cgaaatcgag gaaacggaaa accgcttcga agagcgcaag gtcgatcacc tgcccttcgc
      661 cgccgcgaat atcacggtga tagagcgcga tcatgaccga gagcgagccg aagattcccg
      721 agattaagtc gccgagcgga taacccggcg acatcggcgc tccgtcgggc tcgccgatca
      781 gattggtcag tccgctcatc gcttccgcga cccgaccaaa gcccgcgcga tctttgtagg
      841 gcccggtctg gccgaacccg gagatgcgca gcagaataag ccgcggatcg atgccacgaa
      901 tgacgtccgg cccgatgccc cacttttcaa acgttccggg ccggtaattc tcgacgatga
      961 catcttttcc cttgattagt tgtttgaaaa gcgccgcacc ctccggtttg ccgagatcca
     1021 acgtgataca gcgcttgttg cgcgccaccg atttccacca caacggaata ccgtctttga
     1081 tcggttcgag cttgcgttgg ccgtcgccgt atttgggatg ttcgatcttg attacgtcag
     1141 cgccgaaatc cgcaagcaaa accgtcgcga acggcgccgc gaccatattg cccgcgtcga
     1201 tcacgcgcac gcccttgagc ggcttgtttt cgcttttttt cgccagcggg aaatcgtaat
     1261 gttccttgat gtagtcggcc atcgttcaac cgtcaccctt cgctggcatt tagccataaa
     1321 cagccacgga aataaaccga ggattatggt tccaggtttc aggttcaacg catctcctga
     1381 acgaggtgaa acatccctct gtgctgaagg ttacgccacc gattccccga gtagcgaccg
     1441 ttgcggtcgc gtatcgaggg acgaaacgcg gcgaacgaga aaccgtgaac gcgtcatcat
     1501 ttgacgatcc gcggcagcgt gacttagtat cgtccttgcg taaacaaatt ttttggagga
     1561 gtccaaaatg gcggaacaac cacgaatcgt tacaggtctg ggcaaagcgg agctgcatcg
     1621 cattctcgtg cgcggctacg atttgaacaa ggagttggtc ggcaagatca gctttacgga
     1681 tatgacttcg ctcattctgc gcggccggct gcccacaggc aacgaagcca gaatgcttga
     1741 tgcccttcta gtcattcttg tcgagcatgg catggtttcg catgtcatcg ccgcgcgtct
     1801 catttatcac tgcgcacccg aggcgatgca agccgcggta gccgccgcac tgtgcggcgc
     1861 cggcagcgtt catctcggat catccgagtg gtcggcaaaa atgttgatcg aggcgctccc
     1921 gcgcgatacg caaaatccgg acttggcggc gattgccgcc tcgatttacg atgactactc
     1981 gaagcgcaaa cagcggatgc cgggcatcgg ccatcgcacc cacgccgaag gcgatccgcg
     2041 ggccgacacg ctatttggca ttgcgaaaga gaccggcgtg tatggaagat attgcgacct
     2101 gctccagtca ttgtcgaaag tagcgacgga ccgacgcaaa cggcttatcc ccgtcaatgt
     2161 cacgggagcg attggcgcta ttgccctcga catgggcttt ccctggcaga ttaccaaagc
     2221 ttttgcattg atcggccgaa cactcggtgc aatcgggcac atcgccgaag aaatacagaa
     2281 tccgatggcg acccagatcg atgcggccgt gaagcaagcg atcgtctacg agccagtgcg
     2341 aacctgacga aagaatgttg gaggcggccg gccgcacgga aacacggagg acgcggagaa
     2401 aaagcaaaaa ttagctctgc gtctttgtgg tgaatgcttt tttcttcatt tgctccgttt
     2461 ttccctttct gttgtaatgc gcctggattc aaggagaaac ccatgggcta tcaaaacatt
     2521 ctcaccgagc gggtcgacaa aatcgccgtc attacaatga atcgcccgga gaagctcaat
     2581 gccttgagct acgagctcgc ctgcgaaatc gacgaagaac taaccaaaat agaaagcgac
     2641 gatgaggttc gggcggtgat tctcaccggc gccggcccgc gtgccttctc ggcgggcggc
     2701 gacattatgc agatagtcaa atcgactccg gaagagatgg ccgcgcgcac tgaaactcgc
     2761 cgtgaagcca attggcatct ggcgactttc
//
LOCUS       DASULZ010000394         3127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1062556, whole genome shotgun sequence.
ACCESSION   DASULZ010000394 DASULZ010000000
VERSION     DASULZ010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062556"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            394..1089
                     /locus_tag="VFY96_08135"
     CDS             394..1089
                     /locus_tag="VFY96_08135"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEX5606468.1"
                     /translation="MTNRKNTDDTSVGRRPVWTGIVILVVLFALAAAWKWTPLADLID
                     LRRMSGWIASLRQSPARHVYVLGAYLIGSILLVPITVLILMTAVIFGPVLGSAYSLVG
                     CLAGAAITYAIGLLLGQGLVQRIAGKKWQRLEKKIAQSGIVAVAALRLLPVAPFTVVN
                     VVSGAFKVPLRDYTLGSLLGLAPGILVTNLFAHQLENAIRNPGPGTIVILAALVVITA
                     LGAVWFKRKFATP"
     gene            1091..2377
                     /gene="cls"
                     /locus_tag="VFY96_08140"
     CDS             1091..2377
                     /gene="cls"
                     /locus_tag="VFY96_08140"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEX5606469.1"
                     /translation="MGSRSDWKTPITQITIYGFMVLLVFGCAKVRAIHDVPEISFGEA
                     TSFPTIEALTDASIIGGNKLEILQNGDGTFPIMLRDIQNAKSTITFAQYLVKGGVLAA
                     EFARSFAERCQAGIKVYVLFDSQGSSDAPDELAATMRKPGCQLEYFRRIQAPQVVLPW
                     KLLQYNYRNHRRILVVDGQIGFTGGYALTDDWLGNGVTPNHWRDTNVRIEGPAVRYLQ
                     AAFTESWLETTGNLLGGDGIFPRLESRGRTALQLVKSSPVGGSFQNYILYLLAITWAK
                     KSILITNPYFIPDDHMIRALLKATARGVRVIVLVPATSDFKITYRASRRHYGEMLLGG
                     IEIFEYSPALLHSKTMVVDGVWATVGSTNFDNRSFALNEELNVGVYDRDIAQNLAQIF
                     ATDLKRSRKVTYEEWQARPLKEKFFELFAFPVEEQL"
     gene            2493..2750
                     /locus_tag="VFY96_08145"
                     /pseudo
     CDS             2493..2750
                     /locus_tag="VFY96_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
BASE COUNT          699 a          893 c          806 g          729 t
ORIGIN      
        1 aagccggaat cgacgcccac ataagccacc gccgtgccga tctcccaacc cgcatacgcg
       61 agccggagct ttttcgcgtc ttcggcggcc ctcgcggaat tgaggacacc aacgccaaac
      121 aatgccgtgc agcaaacaaa aataataaat cgccgcaggt gtctcatcgc tccaatgcac
      181 ataaaccgat cataacccac attatcttac cctccaagag ccacgctttt ttccaggatt
      241 gcattgccgc atcaattgca agctgttatc tttttgtatg cgcgtcaaaa aactgccgct
      301 gtctcgacgg aatagctccc ctcaatcgta atactgagca gtaaacggtc ctaacaagtt
      361 tccaaccgca cgttcaatac cctgcccgaa tagatgacga accgtaaaaa cacggatgac
      421 accagcgtgg gccgacgccc cgtttggacg ggtatcgtga tcctggtcgt gctttttgcc
      481 ctggccgcgg catggaaatg gactccgctc gcggatctta tagatctgcg caggatgtca
      541 ggctggattg cttcgctgcg ccagagtccc gcccgacatg tttatgtcct cggtgcttat
      601 ctgatcggca gcattttgct ggttccgatc acagtcttaa ttctaatgac cgcggtcata
      661 tttggaccgg tgcttggatc ggcctattcc ctcgtgggtt gcctggcggg cgcggcaatc
      721 acttacgcca tcggactcct gctcggtcag gggttggtgc aacgaatcgc gggcaagaaa
      781 tggcagcgtt tggagaaaaa aatcgctcaa tccggaattg tcgcggtcgc tgcgttgcgc
      841 cttctgcccg tggctccgtt taccgtggtc aatgtggttt ccggcgcgtt caaggttccc
      901 ttgcgcgatt acacgctggg atcgctgctt gggctcgcgc cgggaattct tgtaacaaac
      961 cttttcgcac accagctgga aaatgccatt cgcaatcctg gcccaggcac gatcgtcata
     1021 ctcgcggcgc tggtcgtcat cactgctctt ggcgcggtct ggttcaaacg aaagtttgca
     1081 acgccatagt atgggctcac gctccgattg gaaaacgcca attactcaga tcaccatcta
     1141 cggctttatg gttctcctgg tctttggttg tgccaaagtc agggcgattc acgatgtgcc
     1201 cgaaatatcg ttcggcgagg cgacttcgtt tccgaccatt gaagcgctga cggacgcttc
     1261 aattatcggt gggaacaagc tcgaaatcct ccagaacggc gatggcacat ttcccatcat
     1321 gctgcgcgat atccaaaacg ctaagtcgac gattacgttt gctcaatacc tcgtgaaagg
     1381 cggcgtctta gccgcagagt tcgcgcgcag tttcgctgaa cgctgccaag ccggcattaa
     1441 agtctatgtc ctgttcgata gccagggcag cagcgatgcg cccgacgagt tggccgcgac
     1501 catgcgcaag cccggctgcc aactcgaata ctttcgccgc attcaggcgc cgcaggtggt
     1561 tcttccatgg aagctcttac aatacaatta ccgcaatcat cggcgcattt tggttgtcga
     1621 cggccagatc ggcttcaccg gtggctacgc cctcaccgac gattggttgg ggaatggcgt
     1681 aacgccgaac cactggcgcg ataccaatgt tcggatcgag ggtccggcgg tccgctatct
     1741 gcaggccgcc tttaccgaaa gctggttgga gaccacaggc aacctcctcg gcggcgacgg
     1801 gattttcccg cgacttgaat cccgcggccg aactgcgctg cagctcgtca aaagttcccc
     1861 tgtcggcggg agctttcaaa attacattct ctacttgttg gccattactt gggccaaaaa
     1921 atcgatcttg attactaatc catatttcat tccggatgac catatgatca gagcgctgtt
     1981 gaaggcgacg gccagaggcg ttcgtgtgat cgtcctcgtt cccgccacga gtgactttaa
     2041 aattacttac cgagcgagcc ggaggcatta tggcgagatg ttactcggcg ggatcgaaat
     2101 ttttgagtat tctcccgctc tcctgcattc aaagaccatg gtcgtcgacg gcgtttgggc
     2161 aacggtgggc agcaccaact ttgataaccg ctctttcgct ctcaacgaag aattgaacgt
     2221 cggtgtgtat gaccgagaca tcgctcagaa tttggcgcaa atctttgcca cggatttgaa
     2281 gcgctcgcgc aaagtcacct acgaagaatg gcaggcccgc ccgcttaaag aaaagttctt
     2341 cgagcttttc gctttccccg tcgaagagca actttgaacg ctattgatcg cgcgtaaatt
     2401 ggccataatt gcaaggctct gaaatttcat ttaggatacg ttcgatttca cgcccgagtt
     2461 gcagttcgtt atcagactgg aggttcggcc taatggcgac gatgattacc agcgagtgca
     2521 ttaactgtgg cgcatgcgag cccgagtgcc ccaacaacgc catcagccag ggtgatcccg
     2581 tttacgtaat tgatccgaag ctatgcaccg aatgtgtcgg gtttcacgat tttgaggcct
     2641 gcgctgcggt ctgccccgtg gatgtctgcg tgaccgaccc gaataacatc gaaaccgaag
     2701 aggcgctcat cgcacgcgct cgcgccatac atcccgaggt caattttccg gaaaatttcg
     2761 aatcgcgctt tcgcaaaggc gaaggaaagc ccacgccggc cgctacgccg ccgagcgcgc
     2821 ccgccgcagc gaaacccgcg agccctgcta tcgctccacg cgccgccgcc gaggcggcga
     2881 caaacgcgtt cgccgacgtc gatgtcaatt acattcaact acccgaaatc gattcttgga
     2941 atattccggt acgctgtttc aaatgtcgtc aggtgcacgt tcaaaatgtc aaaaacttca
     3001 ccatcggcaa tgtgattttc tgtccccatt gcaataagtc gatggtggtc cgcgacaatt
     3061 tgaactatca cattcgcaca ctgctcaagg atgcttacga gaactgggaa agagacagag
     3121 aacaatt
//
LOCUS       DASULZ010000395         2073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1165365, whole genome shotgun sequence.
ACCESSION   DASULZ010000395 DASULZ010000000
VERSION     DASULZ010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165365"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VFY96_08150"
     CDS             complement(<1..367)
                     /locus_tag="VFY96_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX5606470.1"
                     /translation="MAGREKGDGIARRGLMLVLSSPSGAGKTTLSRKLLDEDKNVMLS
                     VSVTTRKMRPGEKDGRDYHFIDRRRFDALVKKNELLEWAEVFDNYYGTPKKPVMEALA
                     SGRDVLFDIDWQGTQQLREK"
     gene            complement(375..1262)
                     /locus_tag="VFY96_08155"
     CDS             complement(375..1262)
                     /locus_tag="VFY96_08155"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX5606471.1"
                     /translation="MALSSMTGFARSHGVAGAYAWAWEIKSVNAKGLDLRFRLPAGWD
                     AVEVPSRARGTEKLSRGTVYANLTVERKGTQPTVKVNEPVLGAVLSALKGLSGKVQAA
                     PPTLDGILSLKGVIEVTEEDESEEEHRAAETAIISGFGEALAALLQMRQAEGKTLGRL
                     LSTRLDEIAVLSARAESAPGRKPEAIKARLAEQVSTLLQASERFDSDRLYQEAVLLAA
                     KADIREELDRLVSHVAQAKKLLANGGAIGRKLDFLAQELHRESNTLTAKANDLELTNI
                     GLDLKGVVEQFREQVQNLE"
     gene            complement(1345..>2073)
                     /gene="mltG"
                     /locus_tag="VFY96_08160"
     CDS             complement(1345..>2073)
                     /gene="mltG"
                     /locus_tag="VFY96_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX5606472.1"
                     /translation="KHASVADVVDTIIEGKVVQHAFTVPEGLTSEQIVARLLENTALA
                     GQIKEIPREGTLLPETYRFTRGMTREQIIQRMQQAHRRVVQDVWERRMPDLPVRTPEQ
                     LVTLASIVEKETGRPDERTRVAAVFVNRLKTKMRLQSDPTIIYGLTGGKGSLGRPIQK
                     NEIEQPTPYNTYVIDGLPPGPIANPGRGSLEAAANPARTKEIYFVADGSGGHLFSDNY
                     TEHQKNVARLRGIEHFNGNQNSRR"
BASE COUNT          388 a          668 c          548 g          469 t
ORIGIN      
        1 ccttttcacg cagctgctgc gtgccttgcc aatcgatatc gaataagaca tcacgcccgc
       61 tcgccagcgc ctccataacc ggtttctttg gcgttccgta ataattgtca aaaacttcgg
      121 cccactcgag cagctcattc tttttgacga gagcatcaaa tcgccgccgg tcgatgaagt
      181 ggtaatcccg cccgtccttt tcgccgggac gcatctttcg cgtcgtgacc gacacagaca
      241 acatgacgtt tttatcttcg tcgagcagct tgcgcgacaa cgtggtcttt ccagcccccg
      301 atggcgaaga gagaaccagc atcaggcccc gccgggcaat cccgtcccct ttttcccgac
      361 cggccatcgt gcgatcactc caagttctgc acctgctcgc ggaactgttc cactacgcct
      421 ttaaggtcta aaccgatgtt cgtgagttcg agatcattag ccttggcggt cagcgtattt
      481 gattcgcggt gcaattcctg cgccagaaag tcgagcttgc gaccgatcgc accgccgttc
      541 gccaagagtt tcttcgcctg cgcgacatgc gaaacaagac ggtcgagctc ctcgcggata
      601 tcggctttgg cagcaagcag gacggcctcc tggtagagcc gatccgaatc gaaacgctcc
      661 gatgcctgga gcaatgtgga cacctgctcg gcaagccgcg cctttattgc ttccggcttg
      721 cgccccgggg cgctctccgc tcgtgcggag agaactgcta tttcatcgag ccgcgtcgac
      781 aacagtctgc ccagagtttt cccttctgcc tgccgcatct ggagcaatgc cgccaatgcc
      841 tctccgaaac cggagataat cgcggtctcg gcggcccgat gctcttcctc gctctcatcc
      901 tcttcggtga cttcaatcac acctttgagc gacaggatgc cgtcgagagt cggcggagct
      961 gcctgcacct ttcctgaaag gcccttgagc gccgaaagga ccgcgccgag gacaggctca
     1021 ttaactttca cagtcggttg cgtgcctttg cgctcaacag tcaggttggc gtagaccgtg
     1081 ccccgcgaaa gtttttccgt gccgcgggca cgcgacggca cctccactgc atcccaccca
     1141 gccggaaggc gaaagcgcaa gtccaggccc ttggcgttca cagatttgat ttcccacgcc
     1201 cacgcgtaag cgccggctac gccgtggctt cgagcaaaac cggtcatgct cgatagagcc
     1261 atggcttcag ggtctcgcgt tgatactttc tcgaaagtgc ggagacctta gccgcgccaa
     1321 ccacacgtca caagagcggc cagtttaccg ccggctgttc tggttgccgt tgaagtgctc
     1381 tatgcctcgc agccgcgcga cgttcttctg atgttcggtg taattgtccg agaacaagtg
     1441 accgccgcta ccgtcggcaa cgaagtagat ctccttggtg cgcgccggat tcgccgcagc
     1501 ttccaatgag ccccgtcctg gattggcaat tggtcccggc ggcaaaccgt cgatgacata
     1561 cgtattgtag ggcgtgggtt gctcgatttc attcttctga attgggcggc cgagcgagcc
     1621 ctttccaccc gtcaaaccgt agatgatcgt cgggtccgac tgcaaacgca ttttggtctt
     1681 gaggcgattc acgaacactg ccgccactcg cgttcgctca tcaggtcgcc cggtttcctt
     1741 ttccacaatc gatgcaagcg tcaccagctg ctccggcgtc ctgaccggca gatcgggcat
     1801 ccggcgctcc cagacatctt gcaccacacg tcgatgcgct tgctgcatac gctggatgat
     1861 ctgctcgcgc gtcattcctc tcgtgaaccg gtacgtttcg ggcaacagcg tcccctcgcg
     1921 cggaatctcc ttaatttgtc ccgcgagggc ggtgttctcc agaagccgcg caacgatctg
     1981 ctcggatgtc agtccctcag gaacagtgaa agcatgctgc accaccttgc cttcaatgat
     2041 cgtgtcgaca acgtctgcta cgctcgcatg ttt
//
LOCUS       DASULZ010000396         3550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_150450, whole genome shotgun sequence.
ACCESSION   DASULZ010000396 DASULZ010000000
VERSION     DASULZ010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150450"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VFY96_08165"
     CDS             complement(<1..218)
                     /locus_tag="VFY96_08165"
                     /inference="COORDINATES: protein motif:HMM:NF028174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase dimerization domain-containing
                     protein"
                     /protein_id="HEX5606473.1"
                     /translation="MVETDNLRELSPRPLMRILGDFANSQILDASIEYDFFTLIHDGS
                     HSAEEIARRAGTDPRATRIVLDSLPALGL"
     gene            complement(220..1014)
                     /locus_tag="VFY96_08170"
     CDS             complement(220..1014)
                     /locus_tag="VFY96_08170"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606474.1"
                     /translation="MGLGRQDFSGKRVAPQEAEAVVADLKKYLAEIADRWLIKGRFHS
                     DMANGSLPMEAIKVFWQNWYGFVAEINNFHGVAYQRHLPFFKRHPELQKHFANHVADE
                     MIHPKPPGHIQIVLEQGRHFGLTNDEMIDYEMLPECRAFLEYYRGVLYEGTMVEWWAS
                     FCVEEAVGHWAKFFGGALRKNYKIPEEKIVYFRMHAEADLEEHDGVMAHADLDWTVLK
                     KMLEEGKAETRPGFNLEYCLRTGTAYFAWFFEGVYQYVKKEGLYKA"
     gene            complement(1082..2584)
                     /locus_tag="VFY96_08175"
     CDS             complement(1082..2584)
                     /locus_tag="VFY96_08175"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5606475.1"
                     /translation="MAVSNPMLSLDEIVAGLIDIVGPQRVSTNPIDKIVYQHTLAYGA
                     YRALPDVIVRIESDSAGQHKVAEILRFANQHKIPIVAKGGVGMGISSPLKGGLLLDML
                     GMDKVLEVNPTMQYVIAEGGASVYAIHGALRKHGLMLPNFGTYGPAVVIGAMINKGGI
                     GYGMTRYGWIADMVLGLEVILANGEAIRLGALANQASTFGPFQKWIHLPDLLGLFTLA
                     AGSMGIISKVCLRAIEGKREWLHDYCYSFRRDQLKNAQDALMQLVKSEIVYDIHFTDR
                     WQYHWPMEEGLYDKGKVPDDGWFFLKTMLMARASDELAYKEKQMREIYRDAGAVDVPE
                     IAEKAMGAKAQEHGLHGWPDWHVMGPDGWCGSVVRHTGMFAVTSKMYPLTFLREMYDL
                     DEGAKRECGLWDAHHSPQHDSYVTRDFAIKEEYFVFYNPYDDEDVAKLRKWMGLVQQF
                     SNERGVVWTAPTLSTKGPLPYGRLGNLYDVIKEIKQLVDPNNILNPGALY"
     gene            complement(2647..3420)
                     /locus_tag="VFY96_08180"
     CDS             complement(2647..3420)
                     /locus_tag="VFY96_08180"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5606476.1"
                     /translation="MADRSNSEALSGKVALVTGASRGIGAAAAIRLAQRGAAVVVNYH
                     QNQAAALKVLDQVRARGARGVIFQADVTRVEEIERMVQAAERDLGAIDILVNNAYFPF
                     QVGPLHELAWGDFHRAVEHELSAFFHCVSACFPKTREKKAGRIIVVSSRLAQQPLPRM
                     GAYAAAKSALESMADTMAIELGPLGITVNVVTPAFTLTDASLIMPEAYRERVKETRPL
                     KKHLYPDDVAGAVAFLAGDEAAMLTGSHILVTGGSHLQL"
BASE COUNT          766 a         1042 c          919 g          823 t
ORIGIN      
        1 agccccaacg ctggcaagct atcaagcaca atgcgggtcg cgcgcggatc agtgcccgcc
       61 cgtcgcgcga tttcttcagc ggaatgactg ccgtcatgaa tcagcgtgaa aaaatcatat
      121 tcgatggaag cgtcgagaat ctggctgttg gcgaaatccc cgaggatccg catcagcggt
      181 cgcggcgaaa gctctcgaag attatcggtt tctaccatgc taagctttgt acaatccttc
      241 ttttttcacg tactggtaca ctccctcgaa aaaccaggca aaatacgcgg tgccagtgcg
      301 caggcaatat tcgagattga atcccggtcg tgtctccgcc tttccttctt ccagcatctt
      361 tttaagcacc gtccaatcga gatccgcatg ggccatgacg ccgtcgtgct cctcgagatc
      421 agcttccgcg tgcatacgga agtagacgat cttttcttcc gggattttgt agtttttccg
      481 tagcgcgccg ccgaagaact tggcccagtg gccgactgct tcttcgacac agaacgacgc
      541 ccaccattcc accatggtgc cttcgtaaag cacaccgcga taatactcga gaaaggcccg
      601 gcactccggc agcatctcgt aatcgatcat ttcatcgttc gtgagtccaa aatgacggcc
      661 ctgctcgagg acgatctgaa tgtggccggg cggctttgga tgaatcatct cgtccgcgac
      721 gtggttggca aaatgttttt gcagctcggg gtggcgcttg aaaaatggca ggtggcgctg
      781 gtaagcgacg ccgtggaaat tatttatttc agcaacgaaa ccgtaccagt tctgccagaa
      841 gactttgatc gcttccatcg gtaagctgcc attcgccata tcgctgtgaa atcgcccctt
      901 gatgagccag cgatcggcaa tctcggccaa atattttttt aaatcggcga ccaccgcctc
      961 ggcttcctgc ggcgccactc gcttaccgga aaaatcctgt cttcctaatc ccatatttcc
     1021 tcctcacgta ggcgctcaat tcgagcgcga ccggctcttt ttaaaacttt gaatagtaac
     1081 ctcagtagag cgcacccggg ttcaaaatat tgttcggatc caccagttgc ttgatctctt
     1141 tgatgacgtc gtacagattg cccagcctgc cataaggtag cggacccttt gtgctcaacg
     1201 tcggcgcggt ccagacgacg ccccgctcat tgctgaattg ttgcaccagc cccatccact
     1261 tccgcagttt ggcgacgtcc tcatcatcgt agggattgta gaacacgaag tactcttctt
     1321 tgatggcaaa atcacgtgtc acatagctat cgtgctgagg tgaatggtgc gcgtcccaaa
     1381 gcccgcattc acgcttggcg ccttcgtcga gatcgtacat ctcccgtaga aacgtcagcg
     1441 ggtacatctt ggaagtgacc gcgaacattc cggtatggcg cacgaccgag ccgcaccagc
     1501 cgtccggacc catcacatgc caatccggcc agccatgcag cccatgctcc tgtgctttgg
     1561 cgcccatcgc cttttcagcg atctcaggga cgtcgacggc gccggcatca cgataaattt
     1621 ctcgcatctg tttttctttg tatgccagtt catcgctggc gcgcgccatg agcatggtct
     1681 ttaaaaaaaa ccagccgtca tcaggcactt tgcctttgtc gtaaagcccc tcctccatcg
     1741 gccaatggta ctgccaccga tcggtgaaat ggatatcgta aacaatttcg ctcttcacga
     1801 gttgcatcag cgcatcctga gcatttttca gctgatcgcg gcgaaacgaa tagcagtaat
     1861 cgtgcagcca ctcgcgcttg ccttcgatgg cacggagaca gactttgctg atgatgccca
     1921 tggagccggc agcgagggtg aaaagcccga gcagatccgg caaatgaatc catttttgaa
     1981 agggtccaaa agtgctcgcc tgattggcca gcgcgccgag ccgaatggcc tcaccgttgg
     2041 cgaggatcac ttctaggccg agcaccatgt cggcgatcca gccgtaccgg gtcatgccgt
     2101 agccgatgcc gcccttattg atcatcgcgc cgatcaccac cgccggtccg taagtgccaa
     2161 agttcggcag catcaaaccg tgttttctga gcgccccatg gatcgcataa acgctcgcgc
     2221 cgccctcggc gatgacatac tgcatggttg ggttcacttc caagacttta tccatgccga
     2281 gcatgtcgag cagtagcccg ccttttaagg gcgagctgat gcccatcccg acaccgccct
     2341 tggcgacgat cggaatcttg tgctggtttg cgaaacggag aatctcggca accttatgct
     2401 ggccagcgct gtcggattcg atacgaacga tgacatcggg cagcgcacgg tacgcgccgt
     2461 aggcgagagt gtgctggtaa acgattttgt cgatcggatt ggtggagact cgttgcgggc
     2521 cgacgatgtc aatcaagccg gcaacaatct cgtcgagcga cagcatggga ttggaaacag
     2581 ccacaatggc taaggggcag gtctgtgcga agacgacctg ttgaactttt aataatccta
     2641 atttttttaa agctgcagat gactgccgcc ggtaactaaa atatggctgc cggtgagcat
     2701 cgcggcttcg tcgcccgcga gaaaagcaac cgcacccgcg acgtcatccg ggtagagatg
     2761 tttttttagc ggtcgcgttt ctttgacccg ctctctatac gcctcaggca taatcagtga
     2821 ggcatccgtc aaggtgaatg caggcgtgac cacattcacc gttattccaa gcggccccaa
     2881 ctcgatggcc atcgtatccg ccattgattc gagcgccgat tttgcggccg cgtaagctcc
     2941 cattctaggc aggggctgtt gcgctagtcg cgagctaacc acaataatcc gtccggcttt
     3001 tttttctctc gttttgggaa agcacgcact gacacaatga aaaaaagccg agagctcgtg
     3061 ctccaccgcg cggtgaaaat cgccccacgc cagctcgtgc agcggcccca cctggaacgg
     3121 aaagtaagcg ttgttgacca aaatatcgat ggcgccaagg tcgcgctctg ccgcctgcac
     3181 catccgttcg atttcctcga ctcgggtcac atcggcttga aaaatgacgc cgcgcgcgcc
     3241 gcgcgctctt acctgatcga gaactttcag agccgcggct tggttttgat gataattcac
     3301 cactacggcg gcgccgcgtt gcgcgaggcg aatcgctgcc gccgcgccaa tgccccggct
     3361 tgcaccggtc accagagcga ccttaccgct gagtgcttcg ctgttcgaac gatccgccac
     3421 agtcacgcgg ttggctttag atggcgacag cgtcctccga cgcgcctttc tcgtatttcg
     3481 cggcgttgtt tttgaatgag agaaatttca tcttcacgta cggcgagcta taaccgttca
     3541 aaattcggtc
//
LOCUS       DASULZ010000397         3910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_352863, whole genome shotgun sequence.
ACCESSION   DASULZ010000397 DASULZ010000000
VERSION     DASULZ010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352863"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(284..1270)
                     /locus_tag="VFY96_08185"
     CDS             complement(284..1270)
                     /locus_tag="VFY96_08185"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX5606477.1"
                     /translation="MWWIRFFDFPRTQIAVIGTVALAVDLAFRTEGGTTAQVIRAALA
                     LCILYQAYEIRPYTIFARKEVEPAKNSRPEGTLSLLLANVKMENRNSARLREIIAQAD
                     PDVILIVEADAWWDSELACFAQSHAFNVRRAQENFYGMLLYSRLELVRSEVRFLIQDD
                     IPSIRAGIKLAEGIEVEIHCLHPRPPVPQETAQSTERDAELIVVGKESKGKQLPIVVM
                     GDLNDVAWSRTNYLFQRISGLIDPRIGRGLYNSFHAGYFFLRFPLDHFFHSTHFRLIE
                     LKRLAYFGSDHFPMFIRLSYEPEAERQQQAPQTHPSDEVEASEKIEEAATGA"
     gene            2302..>3910
                     /gene="ftsH"
                     /locus_tag="VFY96_08190"
     CDS             2302..>3910
                     /gene="ftsH"
                     /locus_tag="VFY96_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX5606478.1"
                     /translation="MRASSKHIVLGLVLALAFFGIFSVLSKQYRPEPEIIFSDFTGSV
                     ERGDVQAVVIQGHNIHGKYKNGELFKTFAPDDPGLVKSLRTKGVKIAAKGEDESPWYM
                     VLLLNWFPMLLLVGVWVFFMRRMQMGGGKVMSFGKSRAKLLTENQQRITFSDVAGVDE
                     AKDDLQEIIAFLKDPKKFTKLGGRLPKGCLLVGAPGTGKTLLARAIAGEAEVPFFSIS
                     GSDFVEMFVGVGASRVRDLFVQGKKKAPCIIFIDEIDAVGRHRGAGFGGGNDEREQTL
                     NQLLVEMDGFESNEAVILIAATNRPDILDPALLRPGRFDRHVVVPRPDVRGREGILQV
                     HIRKVPLSTDVDVSVLARSTPGFTGADLENLVNEAALLAARNDKEKVGMLELELSKDK
                     VMMGAERRSMIISDEEKRTTAYHEAGHALIAKLLPGADPIHKVTIIPRGMALGLTQQL
                     PMDEKYTYPREYLLNNLVILFGGRVAEELVLEQVTTGAGNDIEKATDLARRMVCEWGM
                     SEKLGPMTFGKKEEEIFLGRDFTQKTDF"
BASE COUNT          938 a          989 c         1132 g          851 t
ORIGIN      
        1 gcggacgcta ggccaacctt catatctcga ttcagacgtt acatgaaact tcgagatcac
       61 tcattgcgtg ttaccgggga gtctctaact tgccagccgc gtgggttgaa cggacgccaa
      121 acaatctcaa cagctaggcg gtgacattag ccgtcgtggt cggaccaacg taaaatggtg
      181 aaacagcgat gtcagttccc gatacaggcg ttgtctaggg gcttagccgg agccttttat
      241 agctgaaaag gcatctctac atgccggctg acgcagttat ggcttacgct ccggtcgcgg
      301 cttcttcgat tttttccgac gcctcaactt catccgaagg gtgggtctgg ggcgcctgtt
      361 gctggcgctc cgcctcgggt tcgtagctca agcgaataaa catagggaaa tggtccgagc
      421 caaagtaagc gaggcgcttg agttcgatga gtctgaaatg cgtcgagtga aagaaatgat
      481 cgagcggaaa gcgcaggaag aaataccccg cgtgaaacga gttataaagc ccgcgcccga
      541 tgcgcggatc gattaagccg ctgatccttt gaaataaata gttggtgcgc gaccacgcca
      601 cgtcgttcag atcgcccatc acgacgatcg gcaactgctt tcctttggac tctttgccga
      661 ccacgatcaa ctcggcgtcg cgctcggtgg actgcgcggt ttcctgaggt accggcggcc
      721 ggggatgcag gcagtggatc tccacctcga ttccctcggc caacttgatg ccggcgcgaa
      781 tggaaggaat gtcgtcttgg atcaagaacc gaacctcgga acggaccagt tcgagcctgg
      841 aatagagcag catgccgtaa aagttttctt gggccctgcg cacgttgaac gcatgggatt
      901 gggcaaaaca agcgagctcg ctatcccacc aggcgtccgc ctcgacaatt agaatgacgt
      961 ccggatcggc ttgagcgatg atctcccgga gccgcgccga gttgcgattt tccatcttta
     1021 cattggcgag cagcaagctg agagtccctt ccgggcgcga gttctttgcc ggctcgactt
     1081 ctttcctggc gaaaatggtg tacggtcgaa tttcataggc ttgatagaga atacataagg
     1141 ccaatgcggc gcggatcacc tgcgcggttg taccgccctc ggtgcgaaac gccagatcga
     1201 cagcgagagc caccgtgccg atgaccgcga tttgcgtgcg gggaaagtcg aaaaacctga
     1261 tccaccacac aggcctccgg ctgaggggca gcagggtagc cgctatggct acgaattcga
     1321 gactcgttaa aaaaactccc aacgttcgtt ccccccattc agcataacca ctcgaccgcc
     1381 gcggcggaag gtgggcggag atgactccta cacgcccggt tgcgctccat cgccgcgccg
     1441 ctggcttatg acaaattcgg tgggctccgg atccgggcgt ttgtcttggt cgggatagta
     1501 agggatggca ggaaacccaa aaatatttct ctggctatag attcgacgga aagcggctgg
     1561 ttctaagaag caaggaaagt tttccgagtc agagaaaggc cgcgagtaat taagattgtc
     1621 gtgagacatg gacctgaatc ttcccgtgat tcgcgaagtg aagcggtgaa gcagttctcg
     1681 ggatcacaga atagtgcaga aaacgtggtt caaagggcaa aattgattgc ataaaattta
     1741 gccggcggaa atatcgagca ggtttccctt cggatcgcac atctgatgcg taccgtgcca
     1801 gcaagtccga agcaggtcga tgatcacttg ctattcagac tcgacgttgg gcggctggag
     1861 cgagatattc aggatcgtgt cgcaagaact tcgacatccg ctttgaaagc ctgaaagtct
     1921 tccaacctca aaaccgatgt gatcgaagcc cggccgtgtt gtgttcggtg ccgaggtcat
     1981 cggcaatttt atagcggcgc cgggccgacc caaaggaact ttgtgattgg atagtagagg
     2041 ctcgcataag aggcgtgatt taagaactta gcggtggctt tttaactcca cagcggcatc
     2101 tgaggacaca cgatgccacg cgcgacgaaa ttccgaggcc cgttcaagcc attcacttaa
     2161 aacttcggcc ctttcactct ctttcggtgg gaagtttacc aggatcggcg caagtccatc
     2221 gatgggtatc cttcgctacc cgaaagagct accatcaatc accggccgta taagcgcggc
     2281 gttaactgaa cggaggaatt tttgagggca tcatcaaaac atattgtact tggcttggtg
     2341 ttggcgctcg ctttttttgg aattttcagc gttttaagca aacaataccg gcccgaaccg
     2401 gaaataattt tcagcgactt taccgggtcc gtcgagcgcg gcgacgttca agcagtcgtg
     2461 atccagggac ataatattca cggcaaatac aagaacggtg aactgttcaa aacttttgcg
     2521 ccagatgatc ccgggctggt gaaatcattg cggacgaagg gcgtgaaaat cgctgctaaa
     2581 ggcgaggacg aatcgccatg gtatatggtg ctattgctaa actggtttcc catgctgctc
     2641 ctggttggtg tgtgggtatt ttttatgcgc cggatgcaaa tgggcggcgg caaagtgatg
     2701 tcctttggca agagccgcgc aaaactgctt acagagaatc aacagcgcat aacctttagt
     2761 gatgtcgccg gcgtggatga ggcgaaagat gacctacaag aaatcatcgc attcctaaaa
     2821 gatccgaaaa aattcaccaa acttggcggc agacttccca aagggtgttt gctcgtcggc
     2881 gcgccgggaa ccggcaagac cctgttggct cgcgccatcg cgggtgaagc cgaggtgccg
     2941 ttcttcagca tcagcggctc cgatttcgtg gaaatgtttg tcggcgtcgg cgcttcccga
     3001 gttcgtgacc tgttcgttca aggcaaaaag aaggcgcctt gcattatttt tatcgacgaa
     3061 atcgacgctg tcgggcgtca ccgcggagcc ggttttggcg gcggcaatga cgagcgggaa
     3121 cagacgttga atcagttgtt ggtcgagatg gatggattcg agtccaacga ggcagtcatc
     3181 ctcatcgctg caaccaaccg gcctgacata ttagatcccg cgttgcttcg tcccggacgg
     3241 ttcgatcggc acgtcgtggt accacgccca gacgtcagag ggcgagaagg catcttgcag
     3301 gtgcacatcc gcaaagtgcc gttgtcaacg gacgtggacg tcagcgtcct ggcgcgatcc
     3361 acgcccggat ttaccggcgc ggacttggaa aacctcgtaa acgaagcggc tttgttggca
     3421 gctcggaacg acaaggaaaa agtcggcatg ctcgagttgg agctgtccaa ggacaaggtc
     3481 atgatgggtg cggagcggcg cagcatgatc attagcgatg aggaaaaacg caccacggct
     3541 taccacgaag cggggcatgc cttgatagcg aagttacttc ctggtgccga cccgattcac
     3601 aaagtaacga tcatcccgcg cggtatggcc ttggggctta cgcagcaact tcccatggac
     3661 gaaaaatata cttacccgag ggaatatctc cttaataatc tggtcatcct gtttggtggc
     3721 cgggtggccg aagagcttgt gctcgagcag gtaacgacgg gcgcggggaa cgatatcgag
     3781 aaagctacgg atctggcgcg ccggatggtc tgcgagtggg gaatgagcga aaagcttggg
     3841 ccaatgacat tcggcaaaaa agaagaagag attttcctgg gaagagattt tacgcagaaa
     3901 acagactttt
//
LOCUS       DASULZ010000398         2223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_759198, whole genome shotgun sequence.
ACCESSION   DASULZ010000398 DASULZ010000000
VERSION     DASULZ010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759198"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="VFY96_08195"
     CDS             <1..982
                     /locus_tag="VFY96_08195"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX5606479.1"
                     /translation="DTLMIETTGLADPAPVVQSFFVDERIKSEYQLNGVVTVVDAKHI
                     FQQLSNSPEAKEQIAFADMVLLNKTDLINPEDLPELEFRLRNLNGAARICQTRNSDVD
                     IGTVLDLRSLDVEVRAEKHDHNHRHTDDIETVAISTPGDLDGLKLSHWFRELLAQYGE
                     RIMRMKGILNLRKDPDQFVFQGVHMLFEGRPGRPWAEHEDRLNRLVFIGRDLDRERIT
                     QGFLNCITTSNGASSAEDVDPFGRKQDVSNFTFEQIRYWVQTILSFPPDAPIVIKEVP
                     CVKAGCPPVETAIMVFLKNEPPRTFKILARINEVNFDHVYNLIENPLPCC"
     gene            complement(1170..>2223)
                     /locus_tag="VFY96_08200"
     CDS             complement(1170..>2223)
                     /locus_tag="VFY96_08200"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5606480.1"
                     /translation="KPFGGELKDGVIWGRGALDDKGPGIMELAAMLALKRQKIALKSD
                     VIFLGTADEEAGGALGVGYLLEKYPDLLNNVGVVFNEGGGIRVGEDGRARVYSVSVAE
                     KTPLWLRLTATGTPGHASAPGNDIAVHKLIAALNRIMRYQSPIKVVPEVQKFYADTAQ
                     TAPESRRTGYRDLRQALEDPTFRSQFLADARERASVSNTIAITRITGSDKVNVISGVA
                     TAEIDIRLLPGEEPKTVIDELRRLIEDESIKIEVILSFPAAISPPHPEAMRVVTEFAK
                     SHDPGVPVVAPLVRGFTDCHFFRERGIPCLGFMPHRNPPSSEGLVHGVDERIDLESFN
                     FGIRAMYEIVRKLATE"
BASE COUNT          489 a          640 c          591 g          503 t
ORIGIN      
        1 cgacacgctg atgatcgaaa ccaccgggct ggcggatccg gcaccggtcg tgcagagctt
       61 ttttgtcgac gagcggatca aaagcgaata tcaactcaac ggcgtcgtca ccgtcgtcga
      121 tgccaagcat atcttccaac aattgagcaa ctcgccggaa gccaaagagc aaatcgcttt
      181 tgccgatatg gtgttgctca acaagacgga tttgatcaat ccggaggacc tgccggagct
      241 ggagtttcgc cttcgtaatt tgaacggcgc cgcgcgtatt tgccagacgc gcaattccga
      301 tgtcgacatc ggcacggttt tagatctcag gagtctcgac gtcgaagtca gagcggaaaa
      361 gcacgaccac aatcatcgcc atacggatga catcgaaacc gtggcgatct ccacaccagg
      421 agacctggat ggcctgaagt taagtcactg gtttcgcgaa ttgctcgcgc agtacggcga
      481 gcgcatcatg cgcatgaagg gaattttaaa cctgcgcaaa gatcccgatc agttcgtctt
      541 ccaaggcgtt cacatgctct tcgagggtcg gccgggccga ccgtgggcag agcacgagga
      601 ccgtctgaac cgcctggtgt tcatcggccg cgatttggac agggaaagaa tcacgcaggg
      661 ttttttaaac tgcatcacca ccagcaacgg cgcgtcgtct gccgaagacg tggatccctt
      721 cggtcgcaag caggacgttt cgaatttcac cttcgagcaa attcgttact gggtgcaaac
      781 gattctaagt tttccgcccg acgcgcccat cgtgatcaaa gaagttcctt gcgtgaaggc
      841 cggctgcccg cccgtggaaa ctgcgatcat ggtttttcta aaaaatgagc cgccgcgcac
      901 cttcaagatt ttggcgcgca tcaacgaagt gaatttcgat cacgtttaca atctgataga
      961 aaatcctctt ccctgctgct gagttcgtat tttgctttgc gccgcacctg ggtgaatccg
     1021 tcaaaacgcg tcaagctctc attcggtcat gtggaccgga gcggagatct ttacatttca
     1081 gctgcggacg aaagaagatt tctcctttcg gtcgaaatga cactcgacac ggtctccgca
     1141 ggactgaggc ctactactga agcgatccct cactccgtcg cgagcttccg cacaatttca
     1201 tacatggccc gaatgccaaa gttgaagctc tcgagatcta ttcgttcatc gaccccatga
     1261 accaagcctt cgctgctcgg cggattgcga tggggcatga aaccgaggca cgggatgccc
     1321 ctttcccgaa agaaatgaca gtcggtaaac ccgcgcacca gcggcgctac cactgggacg
     1381 cccgggtcat ggctcttggc gaactcggta acgaccctca tcgcttcggg atggggcggc
     1441 gatatcgcag ccggaaacga caagataacc tctattttga tcgattcatc ctcgatcagt
     1501 cgccgcaatt catcgatgac ggttttcggc tcttcgccgg gtagtaatcg gatatcaatc
     1561 tcggccgtgg ccacgccgga aatcacgttg actttgtccg atccggttat ccgggtgatc
     1621 gcgatcgtat tgcttacgct ggcgcgctca cgcgcatccg cgagaaactg cgagcgaaag
     1681 gtcggatctt ccagtgcctg acgcaaatcg cgatagccgg tgcgccgaga ttcaggcgcg
     1741 gtttgagcgg tatcggcgta gaatttttgc acctcgggaa caactttgat cgggctttga
     1801 tatcgcatta ttcgattaag cgcagcgatc agtttatgca ccgcgatgtc gtttccggga
     1861 gcggaggcat ggcctggtgt tccggttgcc gtaagcctca gccaaagcgg cgtcttttcg
     1921 gccacactca cgctgtaaac tcgcgcgcgg ccgtcttctc cgacacgaat gccgccgcct
     1981 tcgttaaaca ccacgccgac gttgttaagc aggtcgggat atttctccaa aaggtatcca
     2041 actccgagcg cgcccccggc ttcttcgtcg gcggtcccaa gaaagatcac atcgctcttc
     2101 aaagcgattt tttgtcgttt taacgccagc atcgccgcga gttccatgat ccccggccct
     2161 ttgtcgtcaa gcgcgccgcg gccccaaatc actccgtctt tgagctcgcc gccaaatggt
     2221 ttt
//
LOCUS       DASULZ010000399         3392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_150513, whole genome shotgun sequence.
ACCESSION   DASULZ010000399 DASULZ010000000
VERSION     DASULZ010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150513"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1114
                     /locus_tag="VFY96_08205"
     CDS             146..1114
                     /locus_tag="VFY96_08205"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606481.1"
                     /translation="MNWIRFCQGIWLGVTMLLFVSVSNLRGASAQSQKIRISLSSRSN
                     TNTSYYVAQAKGFFKEEGLEIEFIQINPRLGAMAVLNGDVTFTTSFVSTFRGIVQGLP
                     MKTVLVLLKKGVYYLMVRPEIIKDIQDLKGKKLGVTAVNGGDHIIGRELLRMKGVDPN
                     LVQAIAVGDPSLRFQAVITGAVQAVSVPPPYDLTLQQMGLKPISGPPEIGVPSSGLFA
                     SDKLIKENPQILRRTIRAALRANRFIDENREETIRIMTKFVAQTPEIAARSYDVELKA
                     LARDGQMTDSEIEFQMERLADKKRPLDEVRDFSFARQVVKELESGK"
     gene            complement(1247..1492)
                     /locus_tag="VFY96_08210"
     CDS             complement(1247..1492)
                     /locus_tag="VFY96_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606482.1"
                     /translation="MKPFVRYTALFLAIVFLSGAAIVWFDVFTHDNVFTNPELKIAAG
                     WLTTGLMLLALGLRGRRRAAPKTPQVERPIIEVQSKN"
     gene            complement(1586..2590)
                     /locus_tag="VFY96_08215"
     CDS             complement(1586..2590)
                     /locus_tag="VFY96_08215"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5606483.1"
                     /translation="MILIAMVGQARAQAAPFYKDKTIRIIVGFTSGGLYDQYARILAR
                     HMGKHISGNPNIIVQNMPGAGSLSATNYVYGVAKPDGLTLGMPGSGIYLDQLLGRKEA
                     TFDVAKFSWIGSVDQRDLLLYMRAEAPWNSIDDVLNSKEAPKCGSTGTSDLTTIIDNV
                     LEETLGAKFQEVRGYPGGVEIDLALEKGEIHCRGTGITTHFAREPYFTWHKNGFDRHL
                     VQTGNTKDPRLPDAPTLVELMDKKKTPALSRNVARVMLVSATLGRPMIATPGIPGDRI
                     KTLRDAYLKAFKEPEVVEEAKKTKLDLSTLSGEAVETQIREVMNQPKEVIERVKKLSE
                     "
     gene            3153..>3392
                     /locus_tag="VFY96_08220"
     CDS             3153..>3392
                     /locus_tag="VFY96_08220"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX5606484.1"
                     /translation="MIGPDIVTIPGGWFLMGSMHGPENEQPCHRVWVDSFGIGRFPIT
                     NHEYKKIIDGTQKQPPPFWLDPIFSNPEQPVVGVSW"
BASE COUNT          787 a          883 c          922 g          800 t
ORIGIN      
        1 ccagcaggaa tgaaaggatt gcccacttct tcatggaacg gaaatttatt ccaaaccgcg
       61 gcggaatgta aatatcactg cctggattgt tcttgacgct tgccgctgag gtgattattt
      121 tgagccccgg tccgaggaga acattatgaa ctggatacga ttctgccaag ggatttggct
      181 aggcgtcacg atgcttttgt tcgtgagcgt tagtaatctg cggggagcta gtgcgcagag
      241 ccagaaaata cgtatttcgt tatccagccg cagcaatacc aatacgtcgt attacgtggc
      301 tcaggccaaa ggatttttca aagaggaagg attggagatc gagttcattc aaatcaatcc
      361 gcgcctcggc gccatggcgg tactgaacgg tgacgtaact ttcaccacct ctttcgtcag
      421 cacgtttcgc ggcattgtgc agggattgcc gatgaagacg gtgctcgtcc tgctcaaaaa
      481 gggtgtttac tatttgatgg tccgcccgga gatcatcaaa gacattcagg atctgaaggg
      541 aaaaaagctg ggagtcacgg ccgtgaacgg cggggatcac atcatcggcc gagagctcct
      601 gcgcatgaaa ggcgtcgatc cgaatctcgt tcaagcgatt gccgtaggcg atccgtcgct
      661 gagatttcaa gccgtgatca ccggtgctgt tcaggccgtt tccgtgccgc cgccgtacga
      721 tttgaccttg cagcagatgg ggctaaagcc catatcaggc ccgcccgaaa tcggcgtacc
      781 gtcatcgggt ttgttcgcgt cagacaagct gatcaaagaa aacccgcaga tcctccggcg
      841 aacgatacga gccgcactcc gagccaatcg ctttatcgat gaaaaccgag aagaaacgat
      901 acgcatcatg accaagtttg ttgctcagac gccggaaatc gccgcccgat cgtatgacgt
      961 cgaactgaag gcgctcgcgc gcgacggtca aatgaccgac agcgaaattg aatttcaaat
     1021 ggagcggctt gccgacaaga agcggccgct cgacgaggtt cgtgatttct cgtttgcacg
     1081 ccaggtggtg aaagagctgg agagcgggaa gtgagaagaa cggatacttc aggatcgaga
     1141 gtggagatgg aaatcagttg aacaaaacat cgtctaaacg attcggtgtt ttgccgggat
     1201 ttcggtcaaa accggcgccc aggtcggggt tccgcggaat cacgaactag tttttgctct
     1261 gaacttcgat tataggacgc tcgacttgcg gcgttttcgg cgccgcgcgg cggcggccgc
     1321 gcagtccgag ggcgaggagc atcaggccgg tggtgagcca accggcggcg atcttcagct
     1381 ccggattggt gaagacatta tcgtgagtga acacatcgaa ccagacaatc gccgcgccgc
     1441 ttaaaaaaac tatcgccaga aacaaagcgg tgtagcgaac gaaaggtttc acaggtaaag
     1501 cctttaaggc gtcggcgatt tgaaagaaat tgggcgcagc ttgccgagac cctacggtta
     1561 ttcacgtcat cgatgctgac tcgttctatt ccgagagctt cttgacgcgc tcgatgacct
     1621 ctttcggctg attcatcact tcgcgaattt gtgtttccac tgcttcgcca gatagcgtcg
     1681 acaggtcgag cttggttttt tttgcttcct ccacaacttc gggttctttg aatgctttca
     1741 agtaggcatc ccgtagagtt ttgatgcgat ctccgggaat ccccggcgtc gcgatcatcg
     1801 gtcgccccag agttgccgag accagcatca ctctcgcgac attgcggctc agtgcgggcg
     1861 tcttcttttt gtccatgagc tcaacgagcg tcggcgcgtc cggcaaacgc ggatcttttg
     1921 tgttgccggt ttgaacgagg tggcgatcga atccattctt gtgccaggtg aaatacggtt
     1981 cccgagcaaa atgtgttgta atgcccgtgc cgcggcagtg aatctcgcct ttttcaagcg
     2041 ccaaatcgat ttcaacgccg cctggataac cgcgcacctc ttgaaacttt gcgccgagcg
     2101 tctcctcgag cacgttatcg atgatcgtgg ttaaatcgga ggtcccggtg gagccgcatt
     2161 tgggcgcctc cttcgaattg agcacgtcgt cgatagaatt ccacggcgct tccgctcgca
     2221 tgtaaagcaa gagatcgcgt tgatcgacgc tgccgatcca ggaaaatttc gccacatcga
     2281 aagtcgcttc cttacggccc aagagttgat ccaagtatat gccgctcccc ggcatgccga
     2341 gagtcaagcc atccggcttc gcaacgccgt atacataatt ggtggcgctg agagatcccg
     2401 cgccgggcat gttctgcacg ataatgttag ggttgccgga tatgtgcttg cccatgtggc
     2461 gggcgagaat gcgcgcgtac tgatcgtaaa ggccgcccga agtaaaaccg acgatgatgc
     2521 gaatggtttt gtctttgtaa aaaggcgctg cttgggcacg cgcttggccg accatcgcta
     2581 tcaaaatcaa taggactccg accgtttttg tgctccaaca gctgctaccg tggcaaagca
     2641 acgacaattt cattctggcc tcccgtcatg tgaaaacatc tgcttatacg gcaattcttc
     2701 aatccggtgc aatcgccgct ttaattgcat ttgatcagag aaaatcgaat gattcgatga
     2761 cccacagata acactgtcat ttcaagcgcc agattctgtg aaaactcgcc ggtcgtcttc
     2821 gacagagcta aaatttgacg agtagcggaa aatcgtattc ttcgctccgg cagccggtgc
     2881 ctaatcagga cagtcggaac gaataaaccg agttcccgac tactgcgaag cgcgtatcga
     2941 gggaaactcc gttaccggcg cggtctccag cccctcgttc cgcgaccgat gacgctggct
     3001 actcggggaa tcggcagtcc ggtgtgcttt cgtttcgagc ggggaaccat gaaatgaaca
     3061 gccattcaaa ggtttcaaac cttaaccaat tgaaagtgta tttggcgctc ctcgctgttt
     3121 gacaaatttt tccccggttt tagatttact caatgatcgg tcccgatatc gtaaccattc
     3181 ccggcggctg gtttcttatg ggttctatgc acggcccgga gaacgaacag ccctgccacc
     3241 gtgtctgggt ggatagtttc ggcatcggcc gatttccgat cacgaatcat gagtacaaaa
     3301 aaattatcga cggaacgcaa aaacagcctc cgccgttttg gttggacccg atcttttcta
     3361 atccagagca gccggttgtt ggcgtcagtt gg
//
LOCUS       DASULZ010000400         9638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_251865, whole genome shotgun sequence.
ACCESSION   DASULZ010000400 DASULZ010000000
VERSION     DASULZ010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9638
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251865"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VFY96_08225"
     CDS             complement(<1..747)
                     /locus_tag="VFY96_08225"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606485.1"
                     /translation="MKLPVPAAAIIFLLANGTASPAEKIRVGVPQQVVHWMVFPLAQQ
                     KGLFKEEGFDAEIVRITGPSGRSALMSGDIDYYTTIAFIVQSAIVGLPVKVLAAYVNC
                     PPFVLMSRPELKSAQDLKGKTMGIGAPPGSSPDVIAKLSLKHLGLNPDRDVKYVYLNS
                     HERTYLALEQGLYAAGLIPPPFDFKGAKLGLNSLARAADILTYPEDGLIATVNKIKEK
                     PDEVKRVIRAGIKANRYIRTQREGTIQFLMA"
     gene            1178..1333
                     /locus_tag="VFY96_08230"
     CDS             1178..1333
                     /locus_tag="VFY96_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606486.1"
                     /translation="MKVFASVLAVDQGQSFLGAPSFTVPLIGFAMPEIPVFKDIEHSG
                     HAEIKCH"
     gene            1324..1497
                     /locus_tag="VFY96_08235"
     CDS             1324..1497
                     /locus_tag="VFY96_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606487.1"
                     /translation="MSLTDAQSGEFIGASPPSSGASEHDDYTILIVVHFTRTDFEEQR
                     WDLGTDELARTVH"
     gene            1594..2412
                     /locus_tag="VFY96_08240"
     CDS             1594..2412
                     /locus_tag="VFY96_08240"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5606488.1"
                     /translation="MKKASFPRIAHVIITSAVVFTLVLSFHSVAHSACRDPEVARGFG
                     SVAHAAKVDATIQYFGHNFFLITTSKSTRIVTDPLGPGWYPNPNVAADVVTVGKEMFN
                     HNAVQIVRGNPVILRGLKNYGADWNRVSTSFKDTLIYNVPIHQNAEVIEGIHGSAFVF
                     DLGTLCIAHLGDLSQKLNEQQIKSFGKVDVALTPIGGGRTMAPDLAREVLVQLKPKIA
                     IPMHHRDSPYLIQQFSAGMKTRMLNSDTLTVSKDALPSPTEIDVLVPRGAMSYR"
     gene            complement(2454..3998)
                     /locus_tag="VFY96_08245"
     CDS             complement(2454..3998)
                     /locus_tag="VFY96_08245"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HEX5606489.1"
                     /translation="MKNDEISAAWAYHNGTKHSLQSIRVNRHYLDWQNQPLPFKIYRN
                     IEPMRLPEQLSSSGIPALSALSGSTSDEGRSLLTRQSLAEILFLSAGITRRRTYPGGE
                     MLFRAAACTGALYHIDLYIVGALEDLAAGVYQFSPQDFALRKLRDGDFRSVLIQVSGE
                     EPSIATAPCVVVCASTFWRNAWKYQSRAYRHCFWDTGTIVANLLAAASAREILANVVA
                     GFVDTAVSRLLGLDAAREAPIALVALGNTAHPATEAAVFVAPLHIETEPLSATEVDYP
                     AMRRMHEASELQSAAEVKAWRDQTTEAQAKRDGQPIGRSFMLHPLDAAALPNDTVEDV
                     IVRRGSTRAFAREPITFEQLSIVLERATGALSTDFLTGSQPSLNDIYLIVHAVTELPS
                     GAYFFQRDQRVLELFKEGDFRQEAGHLGLGQEIPADCSVNIYFLADLDRALARFGNRG
                     YRAAQLEAGIIGGKIYLAAYAQRLGASGLTFFDDDVTEFFSPHAAGKSVMFLVALGKS
                     AKKRIG"
     gene            4119..4865
                     /locus_tag="VFY96_08250"
     CDS             4119..4865
                     /locus_tag="VFY96_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX5606490.1"
                     /translation="MKRLLVLQHVAHEILGTLDPLLKRAGFRIRYINFSRYPDAQPSI
                     DGYDGLVILGGPMSVNDSDRLPHLSTEMHLIEQAMKRNLPVLGICLGAQLIAKTLGAA
                     VYPNREKEIGWYDIAPTEEARNDPLLSTWAPSEKIFQWHGETFDIPKSTRHLAFSSLC
                     SNQAFSYSSNVYGFQFHLEVEERMIQRWLRVQENQIEIASLEGKINPERIHAETALHI
                     RRLHDLSDRVFGEFIKLFAIEKKFSRLPLR"
     gene            4932..5366
                     /locus_tag="VFY96_08255"
     CDS             4932..5366
                     /locus_tag="VFY96_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX5606491.1"
                     /translation="MDWWIWILLGLVLLLAELVTPGGFYILFFGLGAIAVGLLSAFEA
                     AGPIWFQIVLFSIISVLSLWFFREKLLELTRAPSTDRVDTYIGETAVILDDISVNGMG
                     KAEMRGTPWNTRNVSGRPLARGERCKVERVEGLTIFVRAETT"
     gene            5381..6322
                     /locus_tag="VFY96_08260"
     CDS             5381..6322
                     /locus_tag="VFY96_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HEX5606492.1"
                     /translation="MGGFIVVIALAFLVILVVAKTAVVVPQQSAYVVERLGRYSGTLS
                     AGFHILVPFLDVVRYKHSLKEQAIDIPPQVCITRDNVQVSVDGVLYMKVLNPERASYG
                     IADYIFGITQLAQTTLRSELGKIELDRTFEERTNINTQVVSELDKASEPWGIKVMRYE
                     IKNITPPQDILAAMEKQMRAEREKRALILTSEGQRDAAINSAEGEKQQAIKASEGKKQ
                     QQINEAEGHAAAILAIADATANGIRKVAEAIQQPGGFEAVQLRVAENYLVEFGKLAQT
                     GNTLVLPATLSDVGSMISLAMNVIKQQGNGGGAPAKT"
     gene            complement(6443..7684)
                     /locus_tag="VFY96_08265"
     CDS             complement(6443..7684)
                     /locus_tag="VFY96_08265"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5606493.1"
                     /translation="MSSSSRSTPRLLSPLRHRDFRVFWIGLILSSVGSQFTQIAMAWQ
                     IYELTNSPLQIGLIGLVRAVPQMIILLFGGLLADTMNRRKLMMVTQGSLFFVSGALAL
                     LSLTGQITPGKLYAVTALLALFGSLESPSRQAMVTNLVPAEDLSRALAIFSSQRQIAT
                     IGGPSVAGIVLALAGPAACYAVDAISWLIMLGALALIRTQLSERGSWRTITLNSLRVG
                     FQFVWHHAVIFPFLMMDFGANIFGTVRSLFPIYARDILSVGPEGLGVLYAASAAGALL
                     GAIGFSFWGGVRSAGRWILVGVAVYGVCLLFFAKSHLFWLSIFLLVGSGIGDTISAIL
                     RSTINQLSTPDDLRGRMAAINSIFTNSGPQLGQFQIGALATVAGAEVAAMSGALMILM
                     IVAVLLVRFPQLRNYQLSHVP"
     gene            complement(7748..8623)
                     /gene="mutM"
                     /locus_tag="VFY96_08270"
     CDS             complement(7748..8623)
                     /gene="mutM"
                     /locus_tag="VFY96_08270"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX5606494.1"
                     /translation="MRFFAGCDGLLITYRSLKMPELPEVETVCRSLRPHLLGRTIARV
                     EVIEPRLRMPVNRRVLHRLAGRRVEDIVRTAKYILLHLSGNVVWIFHLGMSGKLICVK
                     GETARRKHDHIIVRLDNGAELRYHDPRRFGLSLVCRREQLLELPQLRHVGIDPFDPRL
                     TADYLFSFTHRSTRRIRDLLLDQQIIAGLGNIYANEILALAGVKPTVRAHRLTRNQVT
                     KIAETIPRLLGDAIRWCGTSFSDYRDADDRSGEFQNHLRVYDRYGERCRTCSRVIKRV
                     AIGNRSGYYCPGCQK"
     gene            complement(8590..9459)
                     /gene="rsmA"
                     /locus_tag="VFY96_08275"
     CDS             complement(8590..9459)
                     /gene="rsmA"
                     /locus_tag="VFY96_08275"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX5606495.1"
                     /translation="MASLYQEVRAALRGSDFRPRKSRGQNFLIHERVIDAIIDLLEPE
                     PNDEVVEIGPGLGFLTRRLLERAGQVWAIEVDRFLAEQLRCSAFGADPKFHLIQGDFL
                     EFEPAEFLPRMPVKLAGNLPYSIAAAVLFRIFEIREQFSWLVLMLQKEVADRIVAKPG
                     TKEFGALSVWCQIHGRVTGKLPVSPEAFFPRPKVRSRVLKIELFSEPRIAPADFPALQ
                     NLVRAAFGQRRKTLGNALAAYFGQPRGAIESWLRSERIDPTRRGETLTVDEFIALAAA
                     ARRCAFLPAATDC"
BASE COUNT         2234 a         2753 c         2632 g         2019 t
ORIGIN      
        1 agccatcaga aattgaatcg tgccctcgcg ctgggtacga atatagcgat tggccttgat
       61 tccggctcga atcacgcgct ttacttcgtc cggtttttct ttgattttgt ttacggtggc
      121 gatcaggcca tcttccggat atgtaagaat gtccgcagct cgggcgagtg agttgagacc
      181 gagctttgcg cctttgaagt cgaagggcgg cggaatcaat ccggccgcat aaagcccctg
      241 ctccaaagcg aggtaggttc gttcatgaga gttcaagtac acgtacttca catccctgtc
      301 cgggttcaag ccgagatgct tgagcgaaag cttagcgatc acgtccggcg aactcccggg
      361 cggcgcgcca atccccatgg ttttcccttt tagatcctgg gccgatttga gctccggtcg
      421 tgacatgagg acgaagggcg ggcaattcac ataagccgcg agaactttga ccggcaatcc
      481 cacgatcgct gactgcacga tgaatgcgat cgtcgtgtaa tagtcgatgt ctccgctcat
      541 cagagcggat ctaccggacg ggccggtgat gcggacgatc tccgcatcga atccctcttc
      601 tttgaaaaga cctttttgtt gcgccaacgg aaaaaccatc caatgaacaa cctgttgcgg
      661 tacgccgacg cggattttct cggccggaga agccgttccg ttggcaagca agaaaatgat
      721 cgccgcggca ggcaccggta gtttcatcgc gcacctccaa tttgaatacg gcgcactgac
      781 gctatcaaag acaacgacgg cagccaagcc tgctatttca gcgcgcttcg atgcagcagg
      841 tttagcgacc gcctactcag gacggtcaga taaaccgatt tccggagtag cgcgaagcac
      901 gcatcgaggg aattttctgc ctcgagcact tcttgagaaa gatatctctg cggctagagt
      961 ctaatacgcc cgcgctccag catcgagcag gaactactcc gttcgggcat tggatcgcgc
     1021 cttggcgaag caggacacac aaaactgacc gacaagatag tcgcgattga ttttacggtc
     1081 ttacaccaga caaggacttc gcgcgagaat attttacagc ttggttgcag tcccagcggg
     1141 tgcggttact tcggatccta gacacgcgca gctcaacttg aaagtcttcg cctcggttct
     1201 tgccgtcgat caggggcaat cattcctggg cgcgccctcg tttacggtcc ctctcatcgg
     1261 cttcgcgatg ccggagattc cggtatttaa agatatcgaa cacagcggcc acgctgaaat
     1321 aaaatgtcac taacggacgc gcaaagtggc gaattcatcg gcgcatctcc tccatcgagc
     1381 ggagcgtccg agcacgatga ttatacaatt ctgatcgtcg ttcattttac tcgcacggat
     1441 tttgaggagc agcgctggga tctgggaacc gacgaactcg cccgaactgt tcactaatgc
     1501 tggctggcga attgaatcgt gcgctcaggc cgccggaagc attgtctttt tggcttcgat
     1561 ccaatagcct gcttcccgta ggtgtgcatc gttatgaaaa aggcatcttt tcccaggatt
     1621 gcgcacgtga tcataacttc ggcggttgtt ttcacattgg tgctttcatt tcactccgtc
     1681 gcgcattccg cttgtcgtga tcctgaggtg gcgagaggct ttggctctgt tgcccatgcc
     1741 gccaaggttg atgcgacgat acaatacttc ggacacaact tttttctgat taccaccagc
     1801 aagagtactc gtattgtgac cgaccctttg ggaccgggat ggtaccccaa ccccaatgtg
     1861 gccgctgatg tggtcacggt cgggaaagaa atgtttaacc acaacgccgt gcagatcgtg
     1921 cgcgggaatc cggtcatcct ccgcgggctc aagaactacg gcgcggactg gaatagggtc
     1981 agcacgagct tcaaggacac cctcatctac aatgtcccga tccatcagaa cgccgaagtg
     2041 atcgagggca ttcatggctc ggccttcgtg ttcgacctcg gcacattgtg cattgctcac
     2101 ctcggcgatt tgagccaaaa gttgaatgag caacagatca aatccttcgg caaagttgac
     2161 gtcgcgttga cgccgatcgg cggcggccgc accatggcac ccgatctggc gcgagaagtt
     2221 cttgtccagc taaaaccgaa gattgcgatt ccgatgcatc atcgcgacag cccctatctg
     2281 attcagcagt ttagcgcggg catgaagaca cgcatgttga attcagacac gctcacggtg
     2341 agcaaagacg ccctgccgag tcccaccgaa atcgatgttt tggttcctcg cggcgccatg
     2401 agctatcgct gatggctaga gcagcaccgt tcattgccgc gagcgcggcg gaactaaccg
     2461 attcgcttct ttgctgattt tcctaacgcg actaaaaaca ttacgctctt cccggctgca
     2521 tgcggcgaga agaactccgt cacgtcatca tcaaaaaaag taagtccgga ggcgccgagc
     2581 cgctgggcgt aagctgcgag ataaatcttt ccgccgataa ttcccgcctc gagctgcgcg
     2641 gcgcggtagc cgcggttgcc gaaccgcgcc agagctcggt cgagatccgc cagaaaataa
     2701 atattgacgc tgcaatccgc cggaatctcc tgaccgagcc cgagatgacc ggcttcctgg
     2761 cggaaatcac cttctttgaa taattccagc acgcgctgat cgcgttgaaa gaagtatgcg
     2821 cccgagggaa gttcggttac ggcgtgtacg atcaaataaa tatcgttgag cgacggctgc
     2881 gacccagtga gaaagtcggt ggaaagagcg ccggtcgcgc gctcgagcac gatcgagagc
     2941 tgctcgaacg tgatgggttc gcgcgcaaat gcacgtgtcg aaccacgtcg aacgatcaca
     3001 tcttcgaccg tatcgtttgg cagggcggca gcgtccaatg gatgcagcat aaagcttcga
     3061 ccgatcggct ggccgtctcg cttcgcttgc gcttctgtgg tttgatcgcg ccacgccttc
     3121 acttctgccg cgctctgtaa ttcggaagct tcatgcattc ttctcatcgc cgggtaatcg
     3181 acttctgttg ccgaaagtgg ctcggtctct atgtgcaacg gcgccacgaa gaccgccgcc
     3241 tcagtcgcag gatgcgctgt gttgcccaga gccaccagcg cgatcggcgc ctcgcgcgcg
     3301 gcatctaagc ccagaagccg gcttaccgcc gtatcgacga agcctgccac aacatttgcg
     3361 agaatttcgc gtgctgacgc cgcggccaat aagttggcga cgatcgtacc ggtgtcccaa
     3421 aaacaatgcc ggtacgcgcg cgactgatat ttccatgcat tgcgccagaa cgtggaagcg
     3481 caaacgacga cgcacggcgc tgttgcgatc gacggctcct ccccagagac ctggatgagc
     3541 actgagcgaa aatcgccgtc gcgaagtttc cgtagtgcaa aatcttgcgg gctgaactga
     3601 tagacgcccg ccgcgagatc ctcaagcgcg ccgacgatat acaaatcgat gtgatagagc
     3661 gcgccggtgc aagcggccgc gcgaaacagc atttcaccgc ccgggtaagt tctgcgccgg
     3721 gtaatgccgg cgctgagaaa cagaatttcc gccaagctct ggcgcgttag aagcgaacgt
     3781 ccctcatctg aagtgctccc ggatagcgcc gagagtgccg gaattcctga agatgacagc
     3841 tgttcaggca agcgcatcgg ctcgatgttg cgatagatct tgaaagggag tggctggttt
     3901 tgccagtcga gataatggcg attgacacga atgctttgga gcgaatgctt cgtaccattg
     3961 tgataggccc acgccgcgct gatctcatcg tttttcatga gaagtccacg taagcaaatt
     4021 cctatcatcg gaaaaccgcg agcgccaccg gccgcctctg ttcgcttgag cattccagtg
     4081 atttcatttc ttttgagtcg cccgttataa ttcttcctgt gaaaaggctc ctcgtcctcc
     4141 agcatgtcgc ccacgagatc ctcggcacac tcgatccttt gctcaagcgc gccggtttcc
     4201 ggattcgcta tatcaatttc tcgcgctatc ccgatgcgca accgagcatt gacggctatg
     4261 acggcttggt gattctgggc ggaccgatga gcgtcaacga ttccgatcgc ttaccgcacc
     4321 tcagcacgga aatgcacttg atcgagcagg ctatgaaaag aaatctgccc gtcttgggca
     4381 tttgcctggg cgcgcagttg atcgcaaaaa ccctcggtgc agccgtttac cccaatcggg
     4441 aaaaggaaat cggctggtac gacatcgcac cgaccgagga ggcaagaaat gatccgttgc
     4501 tcagcacctg ggcaccatcg gaaaaaatat ttcagtggca tggcgaaacc ttcgatattc
     4561 cgaaaagcac ccggcatctc gccttttcct cgctttgctc gaatcaagcg tttagctaca
     4621 gctcgaacgt ttacggtttt cagtttcacc tcgaggtcga ggagcgcatg atccagcgct
     4681 ggcttcgcgt gcaggaaaac cagatcgaga tcgcctctct tgagggaaaa ataaatcccg
     4741 agcggattca cgcggaaacc gcgctccaca ttcgccgctt acacgatctg agcgaccggg
     4801 ttttcggtga gttcatcaag ctgtttgcga tcgagaagaa gtttagccgg ctaccgctgc
     4861 gctgacggta agcacaatcc cctcagcttg aaattcctac ccacctcggc taccttcgac
     4921 cggcacaaaa tatggactgg tggatttgga ttctgctcgg tctcgtgctc ctccttgccg
     4981 agctcgtgac cccgggcggc ttttacattc tcttttttgg cctcggcgcc attgctgtgg
     5041 gcctgctctc tgccttcgaa gccgctggac cgatctggtt ccaaattgtt ctattctcta
     5101 tcatttcggt attgtcactc tggttttttc gggaaaagct tctcgaactc acccgcgcgc
     5161 cgagcacgga ccgagtcgac acctacatcg gcgagacggc ggtgattctt gacgatattt
     5221 cggtcaacgg catgggtaaa gccgaaatgc ggggcacgcc gtggaatacg cgaaatgtga
     5281 gcggcagacc gctcgcccgc ggcgaacgct gtaaagtcga gcgcgtcgaa gggctgacta
     5341 ttttcgtgcg ggccgaaacg acttagagcg gagatcgcat atgggcggat tcatcgtcgt
     5401 catagccttg gcattcctgg tcattctcgt cgtggccaaa accgccgtgg tcgtgccgca
     5461 gcagagcgca tacgtggtcg agcgactcgg ccgctacagc gggactctga gcgccggttt
     5521 tcacatcctg gtgcccttcc tcgatgtcgt tcgctacaag cattcgctca aagaacaagc
     5581 catcgatatc ccgccgcagg tttgcatcac ccgcgataac gtccaggtgt ccgtcgacgg
     5641 cgtgctctat atgaaagtgc tgaaccccga gcgggcatct tatggaatcg ccgattatat
     5701 cttcggcatt acccagctgg cacaaaccac gctgcgcagc gagcttggca agatcgaact
     5761 ggatcgcaca ttcgaagagc gcaccaatat caacactcag gtggtgagcg aattggataa
     5821 agcttcagaa ccgtggggca tcaaagtgat gcgctacgaa atcaaaaaca taacgccgcc
     5881 gcaggatatt ctcgccgcaa tggaaaagca gatgcgcgcc gagcgcgaga agcgcgcgct
     5941 gattttgacc tcagagggcc agagggacgc cgcgatcaat agcgccgaag gcgagaagca
     6001 gcaggcgatc aaggcttcgg aaggcaaaaa gcagcaacag atcaacgagg ctgaaggaca
     6061 tgcagcggcg atcctcgcga ttgccgatgc tacggcgaat ggcatcagaa aagtcgctga
     6121 agcgattcag caaccgggcg gattcgaggc ggtacaactc cgcgtcgcgg aaaactacct
     6181 ggtcgagttc ggtaagctcg cccaaaccgg gaatacgctg gtgctaccgg cgaccttgag
     6241 cgatgtcggc tcgatgattt ccctggcgat gaatgtcatc aagcagcaag gaaatggcgg
     6301 cggcgcgccc gcaaaaactt agtccctctc gctcttatca aaatacgcgt acagcatcgg
     6361 caattggcct cgcggccaag taaagttgcc cgctttattt cccctctttt gaaagagagg
     6421 gtcgcgattt gcccaccggc agctatggca cgtggctgag ttgatagttc ctcaactggg
     6481 gaaagcgcac gagcaatacg gcgacgatca taaggatcat tagcgcgccg ctcatcgcgg
     6541 ccacttccgc ccccgcgacc gtcgccagcg cgccaatttg aaactgcccg agctgtgggc
     6601 cgctattggt aaagatgctg ttgatcgcgg ccattcgtcc gcgtaaatcg tcgggagtgc
     6661 tgagctggtt gatcgtggag cgcagaatcg cgctgatcgt gtcgccgata cctgaaccga
     6721 ccaagagaaa aattgacagc cagaataaat gcgatttggc aaaaaacaac aggcatacac
     6781 cataaaccgc cacgccgacg agaatccacc gtccggcaga acgcactccg ccccagaagc
     6841 tgaagccaat cgcgccgagc aaagcgccgg ctgcgcttgc ggcgtagagc acgcccaacc
     6901 cttccggccc gacgctcagg atatcgcgcg catagatcgg aaacaacgag cggacagtgc
     6961 cgaaaatatt ggcgccgaaa tccatcatca gaaacggaaa gatcacggcg tggtgccaga
     7021 caaattgaaa tcccacccga agcgagttga gggtgatggt acgccagctg ccgcgttcgg
     7081 ataattgggt gcggatcaga gccagcgcgc cgagcatgat caaccaggat attgcatcga
     7141 cggcgtagca cgccgctgga ccggcgagcg ccagcacgat tccagccacc gaagggccgc
     7201 caatcgtcgc gatctgccgc tggctactaa aaatcgctaa cgcgcgcgac agatcttctg
     7261 cgggcacgag gttggtcacc atcgcctgcc gcgagggtga ttccaaagag ccgaataatg
     7321 ccaggagcgc cgttaccgca tacagctttc caggcgttat ctgcccggtt aagcttaata
     7381 gcgccaatgc tcccgaaaca aaaaataaac tgccttgggt caccatcatc aatttgcgcc
     7441 ggttcatcgt gtctgccagc aaaccgccaa acagcaggat gatcatttgc ggcacggccc
     7501 gcactaatcc gatcaatcct atctgtaagg gcgaattggt gagctcgtag atctgccatg
     7561 ccatcgctat ctgggtgaac tggctgccga cgctcgacag gatcagccca atccagaaaa
     7621 cccgaaaatc acgatgccgc agcggcgaga gcagtcgcgg cgttgatcga gaggacgaac
     7681 tcatgaaacc aaattgtcca agtgccgcag caacgtggat ggattagcga ccttaaacct
     7741 tgaacggtta cttttgacaa cctgggcagt agtacccgct gcggttaccg atggccacgc
     7801 gcttgatcac gcgcgaacag gttcgacaac gctcgccgta gcgatcgtag actcgtaaat
     7861 ggttttgaaa ttcccccgag cggtcatcgg catcgcgata atcggaaaat gatgtaccgc
     7921 accagcgaat cgcatcgccg agcaggcggg gaatcgtttc cgcgatcttc gtaacctggt
     7981 tgcgggtgag ccgatgcgct cgcaccgtcg gcttgactcc ggcaagcgca agaatctcgt
     8041 tggcataaat atttccgaga ccggcaataa tctgctggtc cagcaggaga tcgcgaatgc
     8101 gccgcgtgga tctatgcgtg aagctgaaca gataatcggc cgtgagacga ggatcgaacg
     8161 gatcgattcc gacatggcgc aactgaggca gctcgagcag ttgctcacga cggcagacaa
     8221 ggctcaagcc gaaccggcgc ggatcgtgat agcgaagctc ggcgccgtta tcgagcctga
     8281 cgatgatatg gtcatgcttg cgtcgcgccg tctcgccttt gacgcagatc aattttccgg
     8341 acatgcccag gtgaaagatc cagacgacgt tcccggataa gtgaagcaag atatatttcg
     8401 ccgtccgaac gatgtcctcg actcgccgtc ccgcgagacg gtgcagtacg cgccgattca
     8461 ccggcatgcg caaccgaggt tcaatcacct ccacgcgagc aatggttcgc cccagtaggt
     8521 gcggccgtaa gctccggcag acggtctcga cttcaggcaa ttccggcatc ttgagtgagc
     8581 ggtaagtaat cagcaatccg tcgcagccgg caaaaaagcg caccgccgtg cagcagcggc
     8641 caaggcgata aattcatcca ccgttagggt ttcaccgcgg cgggtgggat cgattctttc
     8701 agagcggagc cagctttcaa ttgcaccccg tggctgtccg aaataggctg ccagcgcatt
     8761 gcccaaggtc ttgcgccttt gaccgaacgc cgcgcgcacc aaattttgca aagcgggaaa
     8821 atctgccggt gcgatccgcg gctcagaaaa caattcaatt tttagcaccc tcgaacgcac
     8881 cttcggacgt gggaaaaacg cctcgggtga aaccggtagc ttgcccgtaa cccggccgtg
     8941 aatctggcac caaaccgaga gtgcgccaaa ttccttagtg ccaggtttgg cgacgattcg
     9001 gtcggcgact tccttttgca gcatcaaaac gagccacgaa aactgctcgc gtatttcgaa
     9061 aattcgaaat aatacggccg cggcaatgct gtaaggaagg tttccggcaa gcttgactgg
     9121 cattctcggt aggaattcgg ccggctcaaa ttccagaaaa tcgccttgaa tcaggtgaaa
     9181 cttgggatcg gcaccgaagg cgctgcaccg caattgctcg gccaaaaacc gatccacttc
     9241 gatcgcccac acctgcccag ctcgctcaag cagccgacga gtcaaaaacc caagtccagg
     9301 gccgatctcg acaacctcat cattcggctc gggctcgagt aaatcgataa tcgcgtcaat
     9361 cacgcgctcg tggatcagaa aattctgtcc gcggcttttt ctcggtctaa aatctgagcc
     9421 gcgcaaagcc gcgcgtactt cctgataaag gctcgccata tattgattac gcagctacag
     9481 ataaaaacac gtcgcgggaa aactcgcaac gcttcgcgac cagtggctaa aacgtgccac
     9541 cgtatggctg ttccagcgct gcaagttcac ctacgccttg acacagtttt gcttaatgtt
     9601 tagcaaattt tcagatgact gtttgatagt cttccacg
//
LOCUS       DASULZ010000401         2320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_455550, whole genome shotgun sequence.
ACCESSION   DASULZ010000401 DASULZ010000000
VERSION     DASULZ010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455550"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..267
                     /locus_tag="VFY96_08280"
     CDS             85..267
                     /locus_tag="VFY96_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606496.1"
                     /translation="MRPLRRVGILFPTRSRGAHREFSINGAAASITELGELATIGGKT
                     SFIFSEPDRKWWELSG"
     gene            267..1340
                     /locus_tag="VFY96_08285"
     CDS             267..1340
                     /locus_tag="VFY96_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606497.1"
                     /translation="MKTKHGLQTIVLTVFLLLAHWIALASMAFAQTPFYEGKNLLLLS
                     GTDAGGAGDLRTRAVIPVLKKHIPGHPNIVVQYMPGAGGRKAANHMYTSVRPDGLTVG
                     AMITGMVQGTIFGEKDVLYDLDKFIYLGSHARYAPYAFVTRKAAGFNSRETLIAARGV
                     RIGAPSVGHSLYTVARLFAYMMDMKEPRLVTGYTPREIDFALERNEIDARVSILDSLR
                     TRNAHWLDKDLVHMHATMDIPKGFKHTDRRFAKLPEIEGFARSNQDRRLIQLFRGARD
                     TGQPFVLPPGTPSEHVKILREAMGRAFADPEYIKAYETLTSEPAMTLTWQEFETQLRE
                     LPREAETIDLFKRLSGPAPLPAR"
     gene            complement(1463..>2320)
                     /locus_tag="VFY96_08290"
     CDS             complement(1463..>2320)
                     /locus_tag="VFY96_08290"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606498.1"
                     /translation="YPPSMASVTLMTGIKQKFFEEQGLNPTLLVIASDLALKSQMAGE
                     IDYTLFGGGTGMVAAAQGLPIRVVHLPHQFADLTLVGRPEFKTVAQLRGKKVAVSSFS
                     GAVYSSTRAMLAAGGLDPDKDVVMIPMGREPIRLQALLAGSIDASPLPVPLQAIAEEK
                     GMNLLADIEGKFEVPFSGVTVTERKLRDNPDGVKRFIRAMVKAGRYFLSHRDESVALY
                     MNWLKLSKAIAENAYARSIKTVSPDGLGKESAIKTQLGLIKQTTGKDVKVEAVIDFTL
                     LRQVLAEAK"
BASE COUNT          463 a          705 c          643 g          509 t
ORIGIN      
        1 gccgaccgaa cttgattatc catcaaatga ttacagtacg gggtcaggca tgagtgactg
       61 actctagtcg ggcctcgaac ggcattgcgc ccgctgcggc gcgttggtat tttgttcccc
      121 acgcggagtc gaggagcgca ccgcgaattc tcgatcaacg gcgcggccgc cagcattacc
      181 gaactcggcg aactcgccac cattggcggc aaaaccagtt ttatcttcag cgagccggat
      241 aggaagtggt gggagctcag cggctaatga aaaccaaaca cggactgcaa accatcgtgc
      301 ttaccgtctt tcttctgctc gcgcactgga tcgccttggc ttccatggcg ttcgcccaga
      361 cgccgttcta tgaaggaaag aatcttcttc tcctgagcgg cactgatgcg ggcggtgccg
      421 gcgatctgcg cacgcgcgca gtgattccgg tcttgaaaaa acatatcccc ggccacccta
      481 acatcgttgt gcagtacatg cccggcgcgg gcggacgcaa ggcggccaat cacatgtaca
      541 cgtcggttcg gcctgacggc ttgactgtcg gcgcgatgat tactggcatg gtgcagggca
      601 cgattttcgg cgagaaagac gtactctatg atctcgacaa gttcatttat ctcggctccc
      661 atgcccgcta cgcgccctat gcttttgtta cgcgcaaggc cgcgggcttc aacagccggg
      721 aaactttgat cgcggcccgc ggtgtgcgca tcggcgcgcc gtccgtcggc cacagccttt
      781 atactgtggc ccggctgttc gcctacatga tggacatgaa agagccgcgt ttggtcaccg
      841 gctatacacc gcgggagatc gactttgctt tggaacggaa cgaaatcgat gcccgggtga
      901 gcattctgga cagtttgcgg acgcgcaatg ctcactggtt agataaagat ctggtacata
      961 tgcacgccac catggatatt cccaagggct tcaagcacac cgatcgcagg ttcgccaaac
     1021 ttcccgaaat cgagggtttc gctcgctcga atcaagaccg ccgtctcatc caactttttc
     1081 gcggcgcacg cgacaccggt cagccctttg tcttgccgcc cggcacgccg tcggagcatg
     1141 ttaaaatatt gcgtgaagcc atgggcagag ccttcgccga tccggagtac atcaaagctt
     1201 acgagacact cacttcggag cccgcgatga cgctaacctg gcaggagttc gaaacacagc
     1261 tccgcgagtt gccgcgggaa gccgagacca tcgatctctt caagcggctt tccggcccgg
     1321 cgccgctgcc ggcccggtaa cgacgcgccg tttacgcgtt cgctaacttc agattgttct
     1381 tgctgctatt gccgatggat aggatttgtc cttgtcctac gcccgggcgg agagcgatcg
     1441 ggaggtgtgc gaaggttcct tatcacttcg cttccgctaa cacctgccgc agcaacgtga
     1501 agtcgatgac cgcttcgact ttcacatcct ttcccgtggt ctgtttgatc agcccaagtt
     1561 gggtcttgat cgcgctctct ttgcccaacc catcgggact gaccgttttg atggagcgcg
     1621 cgtaggcgtt ttcggcgatc gctttcgaca gtttgagcca gttcatatag agcgccaccg
     1681 attcatcgcg atgactcagg aagtatcggc cggctttgac catggcgcgg ataaagcgct
     1741 tcactccgtc agggttgtcg cgcagttttc tttcggtcac ggttaccccg ctaaacggca
     1801 cttcgaactt gccttcgata tcggcgagca ggttcatgcc cttttcttcc gcgatggcct
     1861 gcagcggcac aggcagcggg ctagcatcga tactccccgc cagcagtgcc tgcaggcgga
     1921 tcggttcgcg gcccatcgga atcatcacga catctttgtc gggatccaat ccaccggcag
     1981 caagcatcgc tcgcgtggaa ctgtaaaccg caccggaaaa gctgctgaca gcaacttttt
     2041 tgccgcgcag ctgagcgacc gttttaaatt ccggtcgccc taccaaagtg agatcggcaa
     2101 attgatgcgg caggtgcacg accctgatcg gcaagccctg cgctgccgcg accatgcccg
     2161 tgccgccgcc aaacagtgtg taatcgatct cgcccgccat ctggctcttc agagccaaat
     2221 cgctggcgat cacaagcagc gtgggattga ggccttgctc ctcgaaaaat ttctgcttga
     2281 tgcccgtcat cagcgtcacc gacgccatcg acggcggata
//
LOCUS       DASULZ010000402         2327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1267284, whole genome shotgun sequence.
ACCESSION   DASULZ010000402 DASULZ010000000
VERSION     DASULZ010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267284"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            704..1789
                     /gene="dinB"
                     /locus_tag="VFY96_08295"
     CDS             704..1789
                     /gene="dinB"
                     /locus_tag="VFY96_08295"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX5606499.1"
                     /translation="MRIVAHIDMDAFYAAVEEQLNPAFKALPLVVGADPKEGTGRGVV
                     MTANYAARRFGIRSALPISRAWRLAETARQHGEPATLFVRPNMTLYRAVSGRIMDIVK
                     SYGDAFEEASIDEAYLEISSPGDFSTATERMTSLKAEIRNREGIGCSIGIGANKLIAK
                     IASGQEKPDGLTVIAPELVENFLDPLPIRKIPGIGPKSERFLHEQNLRTIGELRRVPA
                     STLVDWFGKWGERLFEKCRGIDTSPVTNEWTRKSLGEQETFEQDTRTQSLVRERLDLM
                     AERIVAKLRAKDFKRFHTVTLTVRFGDFQTSNRSRTIKNGIAVDDNALARLRDEAAAL
                     LAPFFDDRENPRGKAFRLIGLRVEKLL"
     gene            2109..>2327
                     /locus_tag="VFY96_08300"
     CDS             2109..>2327
                     /locus_tag="VFY96_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX5606500.1"
                     /translation="MLKVATWNVNGIRARQAQVLEWIERERPDVICLQELKSTVAQVP
                     AALCELQGYRCYWHGERAYSGVGLHIRRE"
BASE COUNT          553 a          629 c          633 g          512 t
ORIGIN      
        1 aatgcgtagc tgcaccgatt cccgcgcccg ttgccccccc gagtgtcgcg cccgttagag
       61 ttccacaacc tcccgccacg aaactgataa gcgaaatacc gaggaaccat acacgcacct
      121 ttttgttctt cattgatctc acctcctatc tatggaacac tttccgcaaa acgcgttcca
      181 acagacgcag agcgatccaa aaaaacccgt gcaaaacgtt cgtgttgtgg gatgcttagc
      241 gcggtgcaaa acgaattgcc caacccttca ctgattgtgg cggaaagatt ctacggctgg
      301 tgtgggcgat agcgggcgta agtatgggtt tgtctaactg caatcgttca gtggaactga
      361 ggactcttta cctggagcca agtgatgcat tcgtcttcat gttgggcaat gcagtttccg
      421 cacaggctga cggagcaata ttcgcagtcg aattcctcac cgtgaagaat ttcgttccgg
      481 cattcaccgc actcgatggt tccattgatg agctgaaaca taaacccgct gcgcagcgag
      541 atgcggtcga aaaaatcgtt gatctctaaa cttggccgct cacacctgcg gcttaacacg
      601 gttatccttg tctgagcaat gaaaaacttc accggccgtg cgcgaaggtg atgaatcccg
      661 atcgatcgaa aattacccgc tgacgtgaaa caatcgatgg cgcatgcgta ttgttgcgca
      721 tatcgacatg gatgcgttct atgccgcggt cgaggagcaa cttaatccgg cgttcaaagc
      781 gttgccgctc gtggtcggcg cagacccgaa ggaaggcaca ggacggggcg tcgtgatgac
      841 cgcgaactac gctgcacggc ggtttggcat ccgttccgca ttgcccatct ctcgggcatg
      901 gcgtttggca gagaccgctc gccagcacgg cgagcctgca actttgtttg tgcgcccgaa
      961 tatgacgctc tatcgcgcgg tttctgggcg catcatggat atcgtcaaaa gttatggtga
     1021 tgccttcgag gaagcgagca tcgatgaagc ctatctcgag atttcttcac ccggagattt
     1081 ttctaccgcg acggaaagga tgacctcgct gaaagccgag atccgcaatc gagaaggaat
     1141 aggctgttcc atcggtatcg gcgcgaataa actcatcgcc aaaatcgcct cgggccagga
     1201 aaagccggat ggcttgacgg ttatcgcgcc tgagctcgtt gaaaactttc tcgatccatt
     1261 gccgatacga aaaattcccg gaattgggcc caagagcgaa cgtttccttc atgagcagaa
     1321 tcttcggacg atcggcgagc tccgccgcgt gccggccagc acgctggttg actggttcgg
     1381 caaatggggc gagcggctgt tcgagaaatg ccgcggcatt gacacttcgc cagtgacaaa
     1441 tgaatggaca cgaaagtcgc tcggcgaaca agaaactttt gagcaagaca cccgaacgca
     1501 atctctggtc agagagcggc tcgacttaat ggccgagcgg attgtcgcca agctccgcgc
     1561 caaggacttc aagcgttttc atacggtcac cctcacggtt cgtttcggcg actttcagac
     1621 gagcaatcgc tcgcggacga tcaagaacgg tatcgcggta gacgataatg cgctagcccg
     1681 gctgcgtgat gaagccgcag ctctgctcgc gccgtttttc gacgaccgcg aaaatccccg
     1741 cggcaaagca tttcgcttga tcggtttgcg cgtggaaaag cttctctagg ggaaagcaaa
     1801 atttacagaa gtgttttcat gagctcagcg ctcgcccaca gagaacgaaa aaaatggaaa
     1861 gatttctcgc tgcgctcgaa atgacaacga aagcatagct gtcatctcga acccattcga
     1921 tggactcagg gtaaacttcg tgagagatct tgcttcgatt ctgtttccaa acgagacgcg
     1981 cagcacacga aggttggctt ttattccgag cgaggaagac cgtcgcggtg aaactattat
     2041 actgatgatg gcgcggagac gcacgccgat ctcgttgaac atcggcgtgc ctctttctat
     2101 actctcgtat gctcaaggtc gccacctgga acgtgaacgg cattcgtgcc agacaggcgc
     2161 aagttctcga gtggatcgaa cgcgagcgac ctgatgtgat ttgcctacag gagctcaaat
     2221 cgaccgtcgc tcaagtaccg gccgcgttgt gcgagctgca aggatatcgc tgttattggc
     2281 acggcgagcg cgcctattcc ggcgtaggcc tccatattcg acgcgaa
//
LOCUS       DASULZ010000403         3360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1469919, whole genome shotgun sequence.
ACCESSION   DASULZ010000403 DASULZ010000000
VERSION     DASULZ010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469919"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(301..1347)
                     /locus_tag="VFY96_08305"
     CDS             complement(301..1347)
                     /locus_tag="VFY96_08305"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5606501.1"
                     /translation="MKRMVWLMGFILVFAASAVARGQGIRGFAGSGRASGAPGPSGTV
                     VVSPRSNVPAITQGSPRSRQNFTAPTVVGPGNPQIAPGQFHRGGGVFLPPLNGSRRPR
                     LVLGVAPHRLHFPWFFHHPPSVVFIDVPSFDDSTVITQYAPGNVQQEPLIADDSRSDV
                     RTRTRGQLAPFDPTPNAVVDRMLVLATLKKSDVVYDLGCGDGRIVIAAAKKYGVRAVG
                     FEIDPGLAKLARENARHAGVEKLVEIRQQDFLTADLSPASVVTLYLSYDGNLAVRPQL
                     LRQLNRGARVISYAFDMGDWQPKIAESFRDSEGNSHQIYLWQIGAPIIFSDASAQILQ
                     PQPIRKGPLIIDVK"
     gene            complement(1544..2332)
                     /locus_tag="VFY96_08310"
     CDS             complement(1544..2332)
                     /locus_tag="VFY96_08310"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX5606502.1"
                     /translation="MPQPKIQTRLCPHCANSITFDALTCPYCRADLLQSPEPEWPHKD
                     DDWELPPSSVEKGRLTVKSKAILILGLGVFALGVYLVGGNRERRDLGPVLEEQQRSLQ
                     EKDATIKDLESQLATLRQEHQGTRNEIEDLKAKLQESSNDLAEVQKKLAATNREVETF
                     AALRNSGTPRPVARSIEQPAPATSAGSLRGRSGLEPGTYESTRTASVYEAPASSSKIV
                     TQISKGTQLTVVRSVGDWVEIRSKHGNPPGYIRADDVGPISRSR"
     gene            2486..>3360
                     /locus_tag="VFY96_08315"
     CDS             2486..>3360
                     /locus_tag="VFY96_08315"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606503.1"
                     /translation="MALIISEPQTRKLVDMPQAVALLDKMFRDRAAGKMRSVPRRRLK
                     GSEKQLNMMAAWHQDMDLICLRSYAAEANTITLYHGRKGGIQAIINMGFLSSLRTGAA
                     TGVAAKYLAPASSKVLGIIGPGWQATFQVEAVAAACPIEQVVVWGRTPKRRRDFIRQM
                     SKVIKADWQEAMGVDEVEAASDILVVSTDSTTPVATGGSLKDEVLVASIGANATVKHE
                     VSNNLIRRMDLIVVDDLAAARADSGDLVEACQTRIARWEDIVPLEKFVAEGTPQPRPK
                     RIFFQSNGIADEDLAV"
BASE COUNT          736 a          917 c          965 g          742 t
ORIGIN      
        1 gaagccctat ccgctgccgg cggcaatcca aaatgtttac gatctcgatg tggggaagga
       61 tccgaaaatg cgccacatcg gtcccatgga gccttgggat ctacactatc tgagaaaaat
      121 cgacgacagc ggcttcatcg atcggctgta cgcccaataa acttgccgcg tggccaagtc
      181 tcccatgagt gagcttctca tcgatggcga aacgattcat gatagtcgcc ggttggttat
      241 tggcagattg cttatgttgg ctaatcggca caactgcgga atcaaaccgg ccgaatctcc
      301 ctacttgacg tctatgatca gtggaccctt gcgtatgggc tgaggctgta atatctgcgc
      361 gctcgcatca ctgaagataa tcggcgcacc gatttgccag agatagattt gatgtgagtt
      421 gccttccgaa tcgcgaaagg attcggcaat tttgggttgc caatcgccca tatcgaaggc
      481 ataggagatc accctcgcgc cgcgattgag ttgtctgagc agttgcggcc ggaccgcgag
      541 attgccgtcg taggaaagat acaaggtcac caccgacgcg ggagataaat cggcagtgag
      601 aaaatcttgt tgtcggatct cgacaagctt ttccacgccg gcgtgccgtg cattctcgcg
      661 tgccaatttt gccaaccccg gatcgatctc gaaacctact gcgcggacgc catatttttt
      721 cgccgccgcg atgacgattc ttccgtcgcc gcatcccaaa tcgtaaacca cgtcactctt
      781 tttaagcgtc gcgagcacca gcattcgatc gacaactgcg ttcggtgtcg gatcgaaggg
      841 cgccaattga ccgcgtgtgc gtgtgcgtac atcgctccgc gagtcgtcgg caataagggg
      901 ctcctgttgc acgttgcccg gcgcatattg cgttatgacg gttgaatcat cgaaggatgg
      961 aacatcgatg aacaccacgc tgggcggatg atgaaaaaac catggaaaat gtagacggtg
     1021 cggagcgact cccaacacca gtcgcggccg tctcgagcca ttgaggggcg gcaagaatac
     1081 tccaccgccg cgatgaaact gacccggcgc gatctgcgga ttgcccggac cgaccaccgt
     1141 aggagctgtg aagttttgtc tggaccgcgg cgagccctgc gtgatcgctg ggacattgga
     1201 tcgcggcgaa accactactg ttccggatgg acccggtgcg ccggaggccc ggcctgaacc
     1261 ggcgaaacca cggatgcctt gacctcgagc tacagcactc gctgcaaaga ccaggatgaa
     1321 gcccattagc cataccatcc gcttcataaa tcctcccaac gcggaagaat actaggggcg
     1381 cggctagatc aagtcaactg gctgagaact tggtgagatt ggacagagtg gcgaatcctg
     1441 gaggttcgtc gggtacggta atagccgttt atgaagaacg gcgcgagcgg cgctgcaacg
     1501 gcttacaatc agaactgtgc gagagctgaa gggagccgcc ttgctatctc gatcggctga
     1561 tcggtcctac atcatcggcg cggatgtagc cgggtggatt gccatgtttc gaacgaatct
     1621 cgacccagtc gcccacggag cggaccacgg tgagctgagt tcccttgctg atctgtgtga
     1681 caattttcga ggagcttgcc ggcgcctcgt agaccgaggc ggtgcgggtg ctctcatacg
     1741 ttccgggttc caggccgctg cgtccgcgca aagaaccggc agaagttgcg ggtgccggtt
     1801 gctcgatgga ccgcgctact ggtcgcggtg tgccactatt ccgaagcgcc gcgaacgtct
     1861 cgacctcacg gtttgtggcc gccaattttt tttgcacctc agccaggtcg ttgctgctct
     1921 cttgcagttt ggccttgaga tcctcgatct cattcctggt tccttgatgc tcctgccgca
     1981 acgtcgcgag ttgcgattcg agatccttga tcgttgcgtc cttctcctga aggcttctct
     2041 gttgttcttc tagaacgggg ccgaggtcac gccgctcccg gttgccgcca accagataaa
     2101 cgcccagagc aaaaacgccg agtcctagaa tcaaaatcgc tttggatttg acagtaagtc
     2161 ttcctttttc aacagacgac ggtggcagtt cccaatcgtc gtccttgtgc ggccactcgg
     2221 gttctggtga ctgcagcaag tccgcccggc aatagggaca ggtcaaagcg tcgaaggtaa
     2281 tactattcgc gcaatgagga caaagacgag tttgaatctt gggctgtggc attaagacat
     2341 tcggacaggt cgatgtatga tttaaccatt cgtattctga atgttatcaa aactggggtc
     2401 aagctttctt tgttgctttt cgtgatctca agcttgcctc cgttgccagc cttgggctat
     2461 atccacgcag gaaggagttg tacggatggc gctaatcatc agtgaaccgc aaactcgcaa
     2521 gctcgtcgac atgcctcaag ctgtggcttt gctcgacaaa atgttccgcg atcgcgcggc
     2581 cggcaaaatg cgcagtgtgc cgcgccggcg gctcaaaggc agcgaaaaac agctcaacat
     2641 gatggcggcc tggcatcaag acatggacct tatttgcctc cgctcctatg ccgccgaagc
     2701 caacacgatc acgctttatc atggccgtaa aggtggcatt caggcgatca tcaacatggg
     2761 gtttctaagt agcctgcgaa ctggcgctgc caccggtgtt gcggccaagt accttgcgcc
     2821 agccagtagt aaagtcttag gaattatcgg gccgggctgg caagcgacct tccaggtcga
     2881 agcggtagcc gcagcttgtc cgatcgagca agtggtcgtc tggggaagaa cgccgaaacg
     2941 gcgcagagat tttatcaggc aaatgagcaa ggtgattaaa gccgattggc aggaagccat
     3001 gggcgtcgac gaggttgaag cagcctcgga tattttggtg gtgtctacag attcgaccac
     3061 gccggtggcg accggtggaa gtctgaaaga cgaagtttta gttgcctcga tcggcgccaa
     3121 cgccactgtc aagcatgagg tctcaaataa tttgatccgc cggatggatc tgatcgtcgt
     3181 cgacgatctg gcagccgcca gggcggacag cggcgacctg gtcgaggctt gtcagactcg
     3241 tattgctcgt tgggaagaca ttgtcccgct ggaaaagttc gtggccgaag gcacgccgca
     3301 gccccgaccc aaacgcattt ttttccagtc caacggcatc gccgacgaag atctcgccgt
//
LOCUS       DASULZ010000404         3524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_455910, whole genome shotgun sequence.
ACCESSION   DASULZ010000404 DASULZ010000000
VERSION     DASULZ010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455910"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..1196)
                     /locus_tag="VFY96_08320"
     CDS             complement(216..1196)
                     /locus_tag="VFY96_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX5606504.1"
                     /translation="MAPLGYIEVLDSKGNVVERTPIDCFPIHVGRAYSNEVVIDDPYV
                     CPMHIAIELDDRGSLIARDLNSVNGLRLGSGRKKRVSMLALNSGSQFQIGHTILRYRS
                     FDHPLAPTLLDRENGASFFGSPHVAAFAGLLVFALLCLEGFLASVERVTIAGIVSEPL
                     VTFATLFLWAGLWTLAGRVILSRFYFFEHVTIASLAIVGFFAVSAVAEWLEFLFPFIP
                     ALWVAGIFGAGVILAALVFGHLRFASMMRLRSRMAAAFSVSAALIGISFISDYANRSK
                     FSNVMEFTGIVKPLDAAWVPTMSVDRFMENSQKLKKDLETLAQKSKPAQP"
     gene            complement(1184..2473)
                     /locus_tag="VFY96_08325"
     CDS             complement(1184..2473)
                     /locus_tag="VFY96_08325"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX5606505.1"
                     /translation="MKCKLLFSVAFLLLTSFLWAPLNFAQGPITSPTTAEIFKQFSEQ
                     VVKIEVVENGSGAKASVGSGFFANAGGQIVTNYHVVSKLIHSPDRYRIDVVAPSGQIG
                     QAAVLGFDVIYDLAVLRTDQAIKGFLTFETTPVAQGTRLYSLGHPQDLGLTIVEGTYN
                     GLLQHSLYPKVHFTGSLNPGMSGGPTVTHAGKVVGINVATEGEQISFLVPAARALSLL
                     EKTAKIEKPSTANFLEEVGRQIEANQARYLDGMFSNTTPKVTLGPYSVPTKPADFFRC
                     WADALRRKELPYVVISHDCSTDDYIFVSSEQSSGIVRFYHQLLSTEELNPARFFTLYS
                     SQVQAGNVALFGNEEEVTPFRCVTRNLQTQGGKLKGVLCARQYVKLPGLYDAVFRGAT
                     LGARNVGLVTTLSLSGVSFENIQALTRRYAEAIKWRR"
     gene            complement(2627..>3524)
                     /locus_tag="VFY96_08330"
     CDS             complement(2627..>3524)
                     /locus_tag="VFY96_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX5606506.1"
                     /translation="AAEAKAFAAEIKAELEKRGLLGEKLGLGGFDGTAREALINAGIK
                     NIVDTRNLILEARMIKNQDEISCLKTCAAMVDGVWFRIWEGLKPGMRDTDLMTLGSTA
                     GYQLGLESAVPGGWRTGPMTFDRGPHATSRVIQFGDLVYGSYCGATYMGYGSCTYRTF
                     IVGRQPTAKEKDWYKVMRDRIDAIIGEIKPGRTSADAAKHFPPASKWGYKSEVEVLAS
                     EIGHGIGLGTSTGYDAPIINRLWSLDHPQVFEEGMTIAVECREGEHRVGGVRLENMLV
                     VTKTGAEIMDYFPREEILIAPR"
BASE COUNT          827 a         1001 c          944 g          752 t
ORIGIN      
        1 gcccttccaa aaagcgccga agccgacctg ttccgcaagc acaatcagtc cgggtacttc
       61 ggcggccgga tagtgcgccg ctgaattgat ctcgacatcg tagatcatca tggattcctt
      121 ctgagtttcg gttgtgctga agagcgttta aatgaaaaat cgttcgtaca ctagccagtg
      181 gtcggttcta agtcaatcag agccacggcg atagcttagg gctgcgccgg tttagacttt
      241 tgcgcgagcg tttccaaatc cttcttgagc ttttgcgaat tttccatgaa tcgatcgact
      301 gacatcgtcg gcacccatgc ggcatcgagc ggcttcacga taccggtaaa ttccatgaca
      361 ttggaaaatt tgctccggtt cgcgtagtcg gagatgaagc tgattccgat caaagccgcg
      421 ctcaccgaaa atgccgccgc cattcgtgag cgcaggcgca tcatcgatgc gaatcggagg
      481 tggccgaaga ccaaagcggc cagaatgact cctgcgccga aaatccccgc gacccagagc
      541 gccggaatga aaggaaacaa aaactccagc cattccgcga ccgcactgac cgcgaagaat
      601 cccacgatcg ctaaactcgc aatagttacg tgctcgaaaa aataaaagcg gcttaaaatc
      661 acccgtccgg ccaacgtcca caatcccgcc cacaaaaaaa gcgtcgcaaa ggtcaccaga
      721 ggttcactga cgatgccggc gattgtgaca cgctcgacag aggcgagaaa accttcgaga
      781 catagcaagg caaaaaccag taatcctgca aaagcggcga catgaggcga gccaaaaaag
      841 gaggcaccat tttcccgatc cagaagtgtc ggtgccaagg gatggtcaaa acttctatag
      901 cgaagtatgg tatggccaat ttgaaactga ctgccggaat ttagcgccag catcgaaacg
      961 cgcttttttc taccgctgcc taatcgcagc ccattgacgc tattcaagtc gcgcgcaatc
     1021 aaggatccac gatcgtcgag ctcgatcgcg atgtgcatcg ggcaaacata gggatcatcg
     1081 atgacaactt cattgctgta agcgcgcccg acatggatgg gaaagcaatc gataggtgtc
     1141 cgttcgacca cgtttccttt gctgtcgaga acttcgatat agcctagcgg cgccatttaa
     1201 tagcctcggc gtaacgtcgg gttagcgcct ggatattctc gaaactcact ccggaaagac
     1261 ttaacgtcgt caccagacct acgtttcgtg cgcccaaggt cgcgcctctg aacaccgcgt
     1321 cgtaaagacc gggtaacttg acgtattggc gcgcgcacag cacgcccttg agctttccgc
     1381 cttgtgtttg gagattgcga gtgacacagc ggaacggtgt cacctcttcc tcattcccga
     1441 acagcgccac gttaccggcc tggacctggc tcgagtaaag cgtgaagaac cgcgccggat
     1501 tcagctcttc ggtcgaaagc aactggtgat aaaagcgcac aatccccgag gactgctcgc
     1561 tggaaacgaa aatgtaatca tcggtggaac aatcatgact gataacgaca tacggaagct
     1621 ccttgcgccg gagcgcgtca gcccagcagc gaaaaaaatc cgccggcttg gtcggcaccg
     1681 aataggggcc gagggtgacc ttcggtgtgg tgttggaaaa cattccatca agatagcgcg
     1741 cctggttagc ctcaatttga cgtcccactt cctcgagaaa attggccgta gacggttttt
     1801 cgatcttggc ggttttttcc agcaagctga gggcgcgcgc cgccggaacc aaaaagctga
     1861 tctgttcccc ctcggtggcg acgttgatcc cgacaacctt gccggcgtga gtgaccgtcg
     1921 gaccaccgct cataccggga ttgagcgagc cggtgaaatg cactttggga tagagcgagt
     1981 gttgtaaaag accattgtag gttccctcga cgatggtcag accgagatct tgtggatggc
     2041 caagcgaata gagccgcgtg ccctgcgcca cgggtgtggt ttcgaatgtg agaaatcctt
     2101 tgattgcctg atcggtgcgc agcacggcga gatcatagat cacatcaaat cccagcaccg
     2161 cggcctgtcc gatctggccg gatggcgcaa cgacgtcgat ccgatagcga tcgggagaat
     2221 gaatcagctt cgagaccacg tgataattgg taacaatctg acccccggcg ttggcgaaaa
     2281 aaccgctgcc caccgaggct ttggcacccg aaccgttctc gacaacttcg attttgacca
     2341 cctgctcgga aaattgtttg aaaatttccg ccgttgtcgg gctggtaatt ggaccttgag
     2401 cgaaattgag cggcgcccat aggaatgagg tcaaaagcaa aaaagctaca gaaaaaagca
     2461 gtttgcactt catgatctgg cgagaccgca attttggatt tgctgctctt ggcgaggatg
     2521 cggcggacaa agttataaga acggttgcgg ctaatctttc acggaatttg ccaccgcgca
     2581 acttaaagcc gtgagcagct tacgagacgc gaggctgccg cgccgcttag cgcggtgcga
     2641 ttaaaatctc ttcgcgcgga aagtagtcca taatctctgc gccggttttg gtcacgacga
     2701 gcatattttc caagcgcacg ccacccaccc ggtgttcgcc ctcgcggcat tccaccgcga
     2761 tggtcatccc ttcttcgaaa acctgcggat gatcgagcga ccacagccgg ttgataatcg
     2821 gcgcgtcata gccggtgctg gtgccaaggc cgatgccgtg gccgatttcg ctggcgagca
     2881 cttctacttc acttttatac ccccacttgc ttgcgggcgg aaaatgtttt gctgcatcgg
     2941 cgctggttcg ccccggcttg atctcgccga tgatcgcgtc gatgcgatcg cgcatgactt
     3001 tataccagtc tttctccttt gcggtgggct ggcgtccgac gataaacgtg cgataagtgc
     3061 agctgccgta ccccatgtag gtcgcgccgc agtaagagcc gtaaaccaaa tcgccgaact
     3121 ggatcacccg gctcgtcgcg tgcggtccac gatcgaatgt catgggtcct gtgcgccagc
     3181 cgccgggcac tgccgattcc aaacccaact ggtatcccgc cgtcgagccg agcgtcatca
     3241 ggtcggtatc tctcatcccc ggctttaatc cttcccaaat acgaaaccac accccgtcga
     3301 ccatggcggc gcaggtcttc aaacaggaaa tctcgtcctg atttttaatc atccgcgcct
     3361 cgaggatgag gttccgcgtg tcgacgatgt ttttgattcc tgcgttgatc aacgcttcgc
     3421 gcgcggtgcc atcgaaaccg ccaagaccga gcttctcgcc aagcaagccg cgcttttcga
     3481 gctcggcttt gatttccgcg gcgaaagcct tggcctcggc cgcg
//
LOCUS       DASULZ010000405         3509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_759993, whole genome shotgun sequence.
ACCESSION   DASULZ010000405 DASULZ010000000
VERSION     DASULZ010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759993"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="VFY96_08335"
     CDS             complement(<1..902)
                     /locus_tag="VFY96_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020641033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX5606507.1"
                     /translation="MYRVGIDIGGTFTDLLWVGEDGVAVIGKTLTTPGDPSLAVENAL
                     RPVLQASAVQSDRGTLIHGTTLVTNALIERKGAPTALLTTAGFRDAVEIGREHRYELY
                     DLNLDLPKPLVPRHLRFDVPERIAADGSVIQVLDEKFLRRLVGELSANGIKAIAVSYL
                     NSFRNPAHERRTAEIIGEVAPHIRVSLSSEVVAEIREFQRTSTTLVNVYVQERVANYL
                     AQLQARLDQIGFVGNFFIMLSSGGIATRDTASRFPVRLLESGPAAGALAAAQAGLLSG
                     HRDLLSFDMGGTTAKLCVIEDGQPA"
     gene            complement(980..1999)
                     /locus_tag="VFY96_08340"
     CDS             complement(980..1999)
                     /locus_tag="VFY96_08340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5606508.1"
                     /translation="MAKDRVGYALGYGKFTNVREMAEVMKQAEERGFEMAFFSETIEL
                     MRDSVTSLAAIGLATKRLKLGTTQIVRLRGPVVMAQSLATLDELTDGRMTIAPGACTK
                     SHARVHALRQDVGATPPEVLKEYVESMRLLLSGEKCSYHGKYINFDNVGLGWKPIRTH
                     IPIYVPATATAGLKLAGQIADGVVLNAVCSPEYTVNALRIIRESAAAAGRDVTNFEVA
                     QIINCSIEDDHKKALDAVRWEVATKLDPVQISFIAAPKMRVGEPYIRKEDIPLFEKAH
                     AAGGMEGLIKAIPDSYVEGMTASGTPDEVKKRVQQYRDAGVKIPLLRPAAAHQTQRLL
                     DLFAQ"
     gene            complement(2143..>3509)
                     /locus_tag="VFY96_08345"
     CDS             complement(2143..>3509)
                     /locus_tag="VFY96_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606509.1"
                     /translation="YSVWCMVLGCLGTYDFDKGQHVGLLVESWEVKDPKTWLFHLRKS
                     NRFHDGSPVTAEDVVHSLKRTRSDPFTKQFQNVSAVAGEAVVDKYTVRITTKEPTAPL
                     LDYLFDRVIVTSKAIYDKYGPEVADRQHPIGAGPYKFRELIPGQRFVIGKDKSHPMWK
                     QYPQAPDEIVFRLMREPEQRVTALLNNEIQIAQFVPPHLRERVERSANHKIVKFHTAE
                     IMFLAMMPKVKPWDNKLVRQAVGYAIDRDTIIKTLLRGEASRLDGPIGEGQYGYDPNL
                     QPKYNYNPEKARELLKQAGYPNGVDVELSTPVGRYTLDKQVTEAMVPMLTAVGIRTKL
                     LTPEWATLWANVQVGKVPFYYMGRGGVVDPSAALSQYFETGGSPRIGYSNPKLDELLS
                     AERQAFDPAKRKKLLSQAMSLITEEAPAQFMWRHQLLFGMAKNIDYRPLPSERIFGWN
                     MKVR"
BASE COUNT          737 a         1035 c          958 g          779 t
ORIGIN      
        1 gccggctgtc catcttcgat cacgcaaagc tttgcggtcg tcccgcccat gtcgaaggat
       61 agtaaatccc gatgcccgga aagcaggccc gcttgggctg ccgccaatgc gcccgctgcg
      121 ggtcccgatt cgagcagccg aactggaaaa cgcgacgcgg tgtcacgggt ggcaataccg
      181 ccgctcgaca acatgataaa aaaattaccg acaaaaccga tttgatctaa ccgggcttgc
      241 aattgcgcca ggtaattggc gacccgctct tgcacgtaga cattcaccaa ggtcgtgctc
      301 gtgcgttgaa actcgcgaat ctcggcaacc acttcggagc tgagggaaac acggatgtgc
      361 ggcgcgactt ctccgatgat ctccgcagta cgcctttcat gggctggatt gcgaaagctg
      421 ttcaaataag agacggcgat cgctttgatg ccgttggccg ataactcgcc aactaagcgt
      481 cgaagaaatt tttcgtcgag gacttgaatg acactgccat ccgcggcgat tcgctcgggt
      541 acatcgaaac gcaagtggcg cggcactagc ggcttgggaa gatcgaggtt caagtcataa
      601 agctcgtagc ggtgctcccg gccgatctcc accgcatcgc gaaacccggc ggtcgtgagc
      661 agtgccgttg gcgctccttt gcgctcaatc aaggcgttcg tgacgagagt cgtgccatgg
      721 atcagagtac cgcgatcgct ttgcaccgca ctggcttgca aaaccggccg gagcgcattt
      781 tccaccgcga ggctgggatc gcctggggtc gtcagcgttt tcccgatcac agcgacgcca
      841 tcttcgccga cccacaatag gtcggtgaaa gttccgccga tatcgatgcc gacgcgatac
      901 atgtcactta aaaaccgaag gatggatgat tgaagataga ggatggagtc gtttcgcgat
      961 tatcaatccc cgatcctcgc tattgggcaa acagatcgag tagccgttga gtctgatgtg
     1021 ctgctgccgg acggagtagc gggattttga cgccggcgtc gcggtattgc tgcacgcgct
     1081 ttttcacttc atccggagtg ccgcttgcgg tcatcccctc tacataggaa tcggggatag
     1141 ctttgatcaa accttccatg ccgcctgcgg cgtgcgcctt ctcgaaaagc ggaatgtctt
     1201 ccttacgaat gtagggctca ccgacgcgca ttttcggcgc ggcgataaac gagatctgca
     1261 ccgggtcaag cttggtcgcc acttcccagc gcaccgcatc gagcgctttt ttgtgatcgt
     1321 cttcgatcga acaattgatg atctgcgcga cttcaaagtt tgtgacgtcg cgtccggcgg
     1381 cggcagccga ttcgcgaata atgcggagtg cattgacggt gtattcgggc gaacacacgg
     1441 cgttaagaac aaccccgtcg gcgatctggc cggcgagctt caaccccgcc gtcgctgtcg
     1501 ccggcacata gatcggaata tgcgtgcgga tcggcttcca ccccaacccg acgttgtcaa
     1561 aattaatgta tttcccgtga taggaacatt tctcgccgct caacaacaga cgcatggact
     1621 ccacatattc cttgagaacc tccggcggcg tcgcgcccac gtcttgacgc agggcgtgaa
     1681 cgcgcgcatg gctcttggtg caggcaccgg gcgcaatcgt catgcgcccg tcggtgagtt
     1741 cgtccaaggt cgccagcgat tgcgccatta ccaccggccc gcgcaagcgc acgatttgtg
     1801 tggtgccgag cttcaaacgc ttggttgcaa gtccaattgc ggccagtgat gtcaccgagt
     1861 cgcgcatcag ttcgatggtc tcggaaaaaa aagccatttc gaagccgcgc tcctcagcct
     1921 gcttcatcac ctcagccatt tctctcacgt tggtaaattt accgtagccc agcgcgtaac
     1981 cgactcgatc cttcgccatc aagaatgctc cttccactca aacgagcaca caaaattata
     2041 attggatttt caacggaacg cgacgcccga gtaaactgaa gtgtggagag tgttatgatt
     2101 gggcttccca tcacccacca ctccaacact ccataactca agctatctca ctttcatatt
     2161 ccaaccgaag atccgttcgc tgggcaaggg ccgataatcg atgttcttcg ccatgccgaa
     2221 aagcagctga tgccgccaca tgaactgcgc cggcgcttcc tcggtgatca ggctcatggc
     2281 ttgggaaagc aactttttgc gcttggccgg atcgaaggct tggcgctcag ccgacaagag
     2341 ttcatccagc ttcgggttgg aataaccgat gcgcggcgag ccaccggttt cgaagtactg
     2401 ggacaacgcc gcgctcggat ccaccacgcc gccccgaccc atgtagtaaa acggcacttt
     2461 tcccacctgt acgttggccc acaaggtcgc ccattccggg gtgagcaact tggtacgaat
     2521 tccgacagcg gtgagcatcg gcaccatcgc ttcggtaact tgcttgtcca gagtgtaacg
     2581 gccgacgggc gtcgaaagtt cgacgtctac tccgttcggg taacccgcct gtttcagcag
     2641 ctcgcgagct ttctcgggat tatagttgta tttcggctgc aagttcggat catagccata
     2701 ctgtccctcc ccgatgggtc cgtccaagcg agacgcttcg cctcgcagca aagtcttaat
     2761 gatcgtgtcg cgatcgatgg catagccgac cgcttgccgg accagcttgt tatcccaagg
     2821 cttcactttg ggcatcatcg cgagaaacat gatttcagcg gtgtgaaact tgacgatctt
     2881 gtggttcgcg ctgcgctcga cccgctcgcg caagtgcggt ggcacgaatt gggcgatctg
     2941 aatctcgtta tttaagagcg ccgtcacccg ttgctcgggt tcgcgcatga ggcgaaagac
     3001 gatctcatcg ggcgcctgcg gatactgctt ccacatcgga tggcttttgt ccttgccgat
     3061 cacgaaccgc tgtcccggaa tgagctcgcg aaatttgtag ggtcccgcgc cgatcggatg
     3121 ctgtcgatcg gcaacctccg gcccgtattt gtcgtagatc gctttactgg tgacgatcac
     3181 tcggtcgaaa agatagtcga gcaacggcgc cgtcggctct ttcgtggtga ttcgaaccgt
     3241 gtacttgtcg accacggcct cccctgccac cgccgagacg ttctggaact gtttggtgaa
     3301 cggatcgctc cgcgttcgtt tgagcgaatg aaccacatcc tcggccgtga ccggcgagcc
     3361 atcgtgaaag cgattgcttt tccgcaggtg aaacaaccag gttttcggat cttttacttc
     3421 ccagctctcg accagtaagc caacgtgctg ccccttgtcg aaatcatacg tgccgaggca
     3481 gccgagaacc atgcaccata cgctgtaca
//
LOCUS       DASULZ010000406         2475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_51226, whole genome shotgun sequence.
ACCESSION   DASULZ010000406 DASULZ010000000
VERSION     DASULZ010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51226"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2144)
                     /locus_tag="VFY96_08350"
     CDS             complement(<1..2144)
                     /locus_tag="VFY96_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5606510.1"
                     /translation="MLDPTQSTKTVRGFCALCTAHCATIATVVDGKVVRLEADHDHPN
                     GGVFCLKGKAAPELVYHPDRLNYPLKRTKPKGEPDAGWQRISWEQALDEIAHRLLSIR
                     EQFGPRAVALAKGTGGGTSVSDAERWLGRFLNSFGSPNWVSTTHVCNWHKDTGFSYTF
                     GVEIPTPDVAHSNAFLLWGHNPSSTSLILAHDIVAARKRGMKIVAVDPRRVGIAAQAD
                     VLLQVRPGADGALALAMIDVMMREKLFDQMFVRHWTNGTLLIRRDNGAPLTEADLTSD
                     GSPARLVAWDENAKRPVVYDAASGLYDEPSALLALSGERRIATRDGYSVICEPAFARL
                     AALAGQFAPEKSAAITWVPVEKVQQAARLLAANRPVSMFMHNGVGQHTNATQTSRAIA
                     TLYALLGDFDSVGGNVVFPKAPLNAVSGKEFLSKETALERIGRERKPLGPPAKPGNCA
                     AYDVFTAILENRPYPVKALLNFGSNTVMNTGDSQRAREAFRALQFAVAAELFMTPTAE
                     LCDYVLPATSFLEMANVSNDFKHRQQGRVHVQYRPAVVEPVAERRSDTWIIFELAKRL
                     GLGEKFWRGDIATAYNYELAPTGISLAELKASPGGITVSVPPRYQKFAKTDASGKSRG
                     FNTPDKKVAIYSHAFAEHGYAPLPEYIEPIISPMSRPEVASEYPLVLTNAKFTTFIHS
                     QLRGLASLRKASPHPTADIHPETAARYEIADKT"
BASE COUNT          507 a          729 c          697 g          542 t
ORIGIN      
        1 gttttatcgg cgatttcgta acgcgcggcg gtctcgggat gtatgtcggc cgtcggatgc
       61 ggcgacgctt tccgcaggct cgcgagaccg cgcaactggc tgtgaatgaa cgtcgtaaat
      121 ttcgcgttcg tcaataccag cggatattcg ctcgcgactt ccggacggct catcggactg
      181 atgatcggct cgatatattc gggcagcggc gcgtatccat gttccgcgaa cgcgtgtgaa
      241 tagatcgcga cttttttatc aggcgtgttg aagccgcgcg atttcccgct tgcgtcggtt
      301 ttggcgaatt tttgatagcg cggcggcact gaaaccgtga tgccgccggg cgaagctttg
      361 agttctgcga gtgaaatgcc cgtcggcgcc agctcgtaat tgtaggccgt ggcgatatcg
      421 ccgcgccaga atttctcgcc caagccgagc cgttttgcca gctcgaagat aatccaagta
      481 tcggagcgcc gctccgccac gggctccacg accgccggcc tatactgcac gtgcactctt
      541 ccctgctggc ggtgtttgaa gtcgttggag acgttcgcca tttcgagaaa gcttgtggcg
      601 ggaagaacat aatcacataa ttccgccgtc ggcgtcatga acaattccgc ggcaaccgcg
      661 aactgcaaag cgcgaaaggc ttcgcgcgct ctttgcgagt cgccggtatt catcacggtg
      721 ttcgagccga aattcaacag cgctttgacc ggataagggc gattttccag aatcgcggta
      781 aacacatcgt aagcggcgca gttgccgggc ttggccggcg gtccgagcgg tttgcgctcg
      841 cggccgatgc gctcgagcgc ggtttccttc gacaaaaatt ctttcccgga aaccgcgttt
      901 agtggtgcct tgggaaaaac gacgttgccg ccgacggaat cgaaatcacc gagcaaggca
      961 tacagcgtcg caatcgcccg gctcgtctgg gtcgcattcg tatgttgccc gacgccgttg
     1021 tgcataaaca tgctgaccgg acgattcgcg gcgagcaggc gtgccgcctg ttgaactttt
     1081 tcgacaggta cccaggtgat cgcggcggat ttttccggag caaattgccc agccaacgcc
     1141 gcaagccgcg cgaacgcggg ctcgcaaatg acgctatagc catcacgcgt tgcaatgcgc
     1201 cgttcaccgg ataacgcgag cagcgccgac ggttcgtcat acaatccgct cgccgcgtcg
     1261 taaaccaccg gccgcttggc gttctcgtcc caggcgacca agcgcgcagg cgagccgtcc
     1321 gacgtcagat ccgcttcggt aagaggcgca ccgttatcgc gccgaatcaa aagagttccg
     1381 ttggtccagt ggcgcacaaa catttggtca aagagcttct ctctcatcat cacatcgatc
     1441 atcgccaatg cgagcgcgcc gtcggcgccc ggacggacct gcaggagaac atcggcttga
     1501 gcggcgatgc cgacacggcg gggatcaacg gccacaatct tcataccgcg ctttctcgcc
     1561 gcgacgatat catgcgcgag gatcaacgat gtcgagctcg ggttatgacc ccagagcaaa
     1621 aacgcattgc tgtgcgcgac atccggcgtt ggaatttcaa cgccgaaggt ataactgaac
     1681 cccgtgtctt tgtgccaatt gcagacgtga gttgtggaaa cccagttcgg gctgccgaag
     1741 ctgtttagaa aacgtccgag ccagcgttcg gcgtcagaca ccgaagttcc gccgccggtt
     1801 ccttttgcta gagccactgc gcgcggacca aattgctcgc gaatggaaag caatcgatga
     1861 gcaatttcgt caagtgcttg ttcccaactg atcctttgcc atccggcgtc gggctcaccc
     1921 tttggcttcg tccttttaag cgggtaattc agccgatcgg gatgatacac gagctcgggc
     1981 gccgctttgc ctttgagaca aaacactcca ccgttcggat gatcatggtc ggcttcgagc
     2041 cgaacgacct taccatcgac aaccgttgct atcgtcgcac aatgcgccgt gcacagtgcg
     2101 caaaaaccgc gcacagtctt cgtgctctga gtcggatcta acattttgtt ttagactaat
     2161 ccggcacctt gctccttaac cacaggtgca agtattttct ttgtggcgcg ttcaattctg
     2221 tcatttcgag gcgagccgag aaatcttttc gaaggatccc tcagttagtt cgctatgaca
     2281 agagcgaagc tgcaaaatgt cgactgccga aatcgatgct atgacctcaa atacccctgc
     2341 ttccgccaaa acttctcggc cgcgcgatgt agcggaatca cgagcggtcc atccggcgta
     2401 tcgcggcaca tgcgatccag cggcaacgga ccgggtgcct gccagccgat tctttccttt
     2461 ctggcttcca gcgcg
//
LOCUS       DASULZ010000407         2413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1166899, whole genome shotgun sequence.
ACCESSION   DASULZ010000407 DASULZ010000000
VERSION     DASULZ010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166899"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VFY96_08355"
     CDS             complement(<1..878)
                     /locus_tag="VFY96_08355"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5606511.1"
                     /translation="MSRKYRYFSADSHFESLPETWTHRVPAKFRDRAPRRIRLADGRD
                     AILEEGQPLEYRGTNLFAGKSPEEFNPVHLNFEGSVGAGSPQQRIREQDADGIDGELL
                     FASEARNPKIKDKEAFLAIIRAFNDYFIEEYCAVDPDRLIGVAVMPDIGAEENIAEMK
                     RCKEKGFKAVRLHTFPSGKATPSPEDDKFYAAALDLDMPITIHTSFPRRTDERDVYLM
                     KYPKDPQGEERPFDFLQRFARQGIYHCGAVEATQLIFSGVFDRFPKLQIYWAENNIGW
                     IPYFYQQMDQTYKVNCS"
     gene            complement(1051..2133)
                     /locus_tag="VFY96_08360"
     CDS             complement(1051..2133)
                     /locus_tag="VFY96_08360"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5606512.1"
                     /translation="MASKKILVGTIAVLLLICGAMSGADSASVEGLLGDINKLPAPQR
                     QARLEEGARREGAFVYYSISNADLVAAYVKGFTARYPFVKGDYYRGSGNQLVVRTLME
                     HRAGRLAADVVSVGTENVMQLKRAGIWTPYQSPETTNYPREQNDKDGYFHADSLGLAT
                     IAYNRDLVRKEDAPKGYADLLDPKWKGSLTIDLEPERAMLTWLSAWGEAKTREYVQKL
                     LANGASVRRGHTLQGQLMCAGEFKIAVEVYPDAILRMKQKGCPAEIVFPNPTPAIAGG
                     NYGINVNAPHPHAAALFVDYALSAEGAKILSGTGRIPRRKGARSIYDELNNLEEKGVN
                     LHIVTAEQTDRLSPAMEKIMKEMLVR"
     gene            complement(2154..>2413)
                     /locus_tag="VFY96_08365"
     CDS             complement(2154..>2413)
                     /locus_tag="VFY96_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606513.1"
                     /translation="FVPPEMWFHQHFNGGAEPVFFLAIGWGSDKPKAGGKAYVYKSVK
                     EGGDQIEYEDEDPMIHAEFEAAMNKAGARCKMDYHPYCTMK"
BASE COUNT          508 a          699 c          628 g          578 t
ORIGIN      
        1 gagcaattga ccttataggt ttgatccatc tgctggtaaa agtatggaat ccaaccgatg
       61 ttgttttccg cccagtagat ttgcagtttg gggaaacggt cgaacacgcc gctgaagatc
      121 aattgtgtgg cttccaccgc gccgcagtga taaatcccct ggcgcgcgaa gcgctggaga
      181 aaatcgaatg ggcgctcctc gccctgcggg tccttcggat acttcatgag atacacatcg
      241 cgctcgtcgg tgcgccgtgg aaatgaggtg tgaatcgtaa tcggcatatc gaggtcgagc
      301 gccgccgcgt agaacttgtc gtcctcagga ctgggcgtgg ccttgccgct cggaaacgta
      361 tgcagccgaa cggccttgaa gcccttttct ttgcagcgct tcatctccgc aatgttttct
      421 tctgcgccga tatccggcat gacggcaacg ccgatcaagc gatcgggatc gaccgcgcaa
      481 tactcctcga taaagtaatc attgaacgcg cggatgattg ccaaaaaagc ttccttatcc
      541 ttgatcttcg gattgcgcgc ttcgcttgcg aacaacaact cgccatcgat cccatcggca
      601 tcttgctctc tgattcgttg ctggggagag cccgccccta cggagccttc gaaattcaaa
      661 tgcacgggat tgaactcttc gggcgatttt ccggcgaaca ggttcgtacc gcgatattcg
      721 agcggctgtc cctcttcgag aatggcgtca cgaccgtccg cgagcctgat ccgccgcggc
      781 gcgcgatcgc gaaactttgc cgggactcgg tgggtccagg tttccggcag cgattcaaag
      841 tggctatcgg ctgaaaaata ccgatatttg cgcgacattt aagcagaacc tcctaacgaa
      901 aatataaatc ggagatgcca cttagccgcg gagagcacag aggtcggatt tctaacagag
      961 tgcaattcat ctctcccgaa tctctgggtc ctcagcgcct ctgcggtgta tattccgatt
     1021 ttgaatctcg taccatccac gggcgcggcg ctaccgcacc agcatttctt tcatgatttt
     1081 ttccatcgcg ggtgacagcc gatcggtttg ctccgctgtg acgatgtgca ggttcacacc
     1141 tttttcttcg agattgttca attcgtcgta aatggatcgc gctccctttc gacgcggaat
     1201 tctgccggtg ccggaaagga tcttggcgcc ttcggcggac aaggcataat ccacaaaaag
     1261 agccgcggca tgcggatgcg gcgcgttgac gttgatgcca taattcccgc cggcaattgc
     1321 cggagtcgga ttagggaata cgatttcggc cggacaacct ttttgcttca tgcgcaaaat
     1381 ggcatcagga tagacttcga cggcaatctt aaattcgccg gcgcacatga gctgtccttg
     1441 cagcgtatgg ccacggcgca ccgaggcgcc attggcgaga agcttctgta cgtattcgcg
     1501 agtttttgct tcaccccatg ccgacagcca ggtgagcatc gcgcgctctg gctcgaggtc
     1561 tatggttaaa ctgcccttcc atttcgggtc gagcaaatcc gcataacctt tcggcgcatc
     1621 ttctttccgc acgagatctc ggttataggc aatcgtcgcc aacccaaggc tatccgcatg
     1681 aaaatatccg tctttatcgt tctgctcgcg cggataattc gtcgtctctg gggattggta
     1741 aggcgtccag atcccggcgc gcttgagttg catgacgttt tcggtgccga cggaaaccac
     1801 gtcagcggcg agccgccccg ctctatgctc catcagcgta cgcacgacca attggttgcc
     1861 gctaccgcgg taataatcgc ccttgacgaa cggataccgt gcggtgaatc ctttgacata
     1921 cgcggcgacc agatccgcat tggaaatgga atagtagaca aacgctccct cgcgccgtgc
     1981 gccctcttcc aatcgtgcct ggcgctgtgg cgcaggaagc ttgttgatgt cgccgagcaa
     2041 tccttccact gaagcagagt cagcgcctga cattgcgccg cagataagaa gtaacaccgc
     2101 aattgtcccc accaggattt tcttcgacgc catcttctat cctcgactgt cgattacttc
     2161 atcgtgcagt atggatgata atccattttg caccgggcac cagccttgtt catcgccgcc
     2221 tcgaactccg catgaatcat cggatcttcg tcttcatact cgatttgatc gccgccttcc
     2281 ttcacgctct tgtagacgta agccttgccg ccggctttcg gtttgtcact gccccaacca
     2341 atggcgagaa aaaacaccgg ctcagcgccg ccgttaaaat gctgatggaa ccacatctcc
     2401 ggcggcacaa aga
//
LOCUS       DASULZ010000408         3124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_151924, whole genome shotgun sequence.
ACCESSION   DASULZ010000408 DASULZ010000000
VERSION     DASULZ010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151924"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="VFY96_08370"
     CDS             complement(<1..1118)
                     /locus_tag="VFY96_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008075337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX5606514.1"
                     /translation="MSYAIAVDIGGTFTDLVAFDRESRRVIYAKSPTTYENLVDGILE
                     CFRKAKIRPAEAALVNHGTTLVINALIQRKGAKTALVTSKGFRDILEIARGNRPDPFD
                     LHYQRDEPLIPRELRFEVPERLDSKGEVVTPLDKPALKKLADEIKKNGIESVAIFFMN
                     SYINPAHEENATEQLRELLPDTYITFSTDLTREWYEYERCSTVAANAYVGPLVSTYIR
                     RLDNDFKREGFDGSLFMMGSNGGLLSAEHTCRQAIGLVESGPIGGCIGASAYAEKLGF
                     KNVVAFDMGGTTAKCALVENGRFSVNSVYYVNGYVKGFPIKSAVIDIVEVGSGGGSIA
                     WMDAQNRLHVGPQSAGSTPGPVCYGRGGTEPTITDANLV"
     gene            complement(1203..2939)
                     /locus_tag="VFY96_08375"
     CDS             complement(1203..2939)
                     /locus_tag="VFY96_08375"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX5606515.1"
                     /translation="MPQLLDKRTDIDPITLEVIRHGIISICDQIDANMTRTAFSPYIY
                     EYKDYAVGFVGKAGELLAQSTGGMPVFVADSVGMAVRDGLETYGRENLHKGDVIVCNH
                     AAVQGQHLNNTVMYTPVFAGTNGDELIGFFSINCHWIDIGGGAIGSVSYSSTDIFMEG
                     LQLRSIKILSKGEPIEEVYRIIENNTRFPLELLGDIEAQLGGCLLGRDLTQAMADKYG
                     TSVFLDALARILDQCEAATREKIRAITDGVYRHEAYLDNDGVRDEKIPIRVKVVIAGD
                     EMTVDFSEMSEQVKGCINSGFYGGGRTCVRVAFKYLIATDEPANEGTFRPVKMILPQG
                     KLISAEPTAAMGLYSIPFPTVIDCIIKALEPALPERVTGAHFGTFSSLSFAGKRTDTG
                     AVFKANDSGHGGWGACATHDGAGPFRTMAHGDTRLIPIELQESMYPFRVEEFCLRQDS
                     GGPGTWRGGLGFDKQYVLLAPCELWANFDRIGCPPWGVQGGKSGKSGQVLIYKNGDQK
                     PELLYKTENRPLQAGDRVRMSTGGGGGYGDPRQRAIESVLRDVTRGFVSLESARDEYG
                     VMVDANGNARRK"
BASE COUNT          665 a          901 c          851 g          707 t
ORIGIN      
        1 accaaattcg catcggtgat ggtcggctcg gtgccaccgc gcccatagca gaccggaccg
       61 ggcgtcgatc ccgcgctttg cgggcccaca tgcaaacgat tttgcgcatc catccatgcg
      121 atcgagccgc cgcctgaacc gacttcgacg atgtcgatga ccgccgattt gatcggaaaa
      181 cctttgacat agccgttgac ataatagacg gagttcacgg agaaacggcc gttctcaacc
      241 aacgcgcatt tcgcggtggt gccgcccatg tcaaacgcca cgacgttttt aaacccgagt
      301 ttctcggcgt aagcactggc gccgatgcag ccgccgatgg gacccgattc gaccagaccg
      361 atcgcctggc gacaagtgtg ctcggcggac agtaacccgc cgttcgagcc catcataaag
      421 agcgatccat cgaagccttc gcgcttgaag tcgttgtcga ggcggcggat gtaggtgctc
      481 accagcggtc ctacgtaagc attcgccgcg actgtcgagc agcgctcgta ctcataccac
      541 tcacgcgtca gatcggtact gaacgtgata taggtgtcgg gcaacagctc tctgagctgc
      601 tccgtagcgt tctcctcgtg agccgggttg atgtaggagt tcataaaaaa gatcgccacg
      661 gactcgatgc cgtttttctt gatctcatcg gcaagcttct ttaaagctgg tttatcgagc
      721 ggcgtgacaa cctcgccttt gctgtccagt cgttccggta cttcgaaccg cagctcgcgc
      781 ggtatcaagg gttcatcgcg ctgataatgt agatcgaacg gatcgggccg gttaccgcgg
      841 gcgatttcaa gtatgtcgcg gaaacctttg ctcgtcacca gcgcggtctt cgcgcctttg
      901 cgttggatca atgcattgat caccagcgtc gtgccgtgat tgaccagcgc tgcttctgcg
      961 ggcctgatct tcgctttgcg gaaacattcg agaatcccgt ccacgaggtt ttcgtacgtg
     1021 gtcgggcttt tcgcataaat gacgcgccga ctttcacgat cgaaggcgac gagatcggta
     1081 aacgtgccgc cgatatccac ggcaatggcg taagacattc tgactcctgc ttagtgattg
     1141 gagtttagag tctcgccgag actgagctga actcgaatcc cgtggacgcg aggcgctaga
     1201 ccttatttgc ggcgcgcatt gccgtttgcg tcaaccatga cgccatactc atcgcgcgcg
     1261 ctttcaagtg agacaaagcc gcgcgtcaca tcacgcaaga ccgattcaat ggcgcgctgt
     1321 cgtggatcgc cgtaaccgcc gccgccgccc gtcgacatgc gaactcgatc accggcttgg
     1381 agaggccggt tttcggtttt gtaaagcagc tcaggctttt gatcaccatt tttataaatc
     1441 agcacttgac ccgactttcc ggatttgccg ccctgtaccc cccagggtgg acagccgata
     1501 cggtcgaagt tcgcccatag ctcgcaggga gccagaagca cgtattgctt gtcaaaaccc
     1561 aaaccgccgc gccacgttcc aggtccgccg gaatcttgac gcagacagaa ctcttcgaca
     1621 cgaaaggggt acatggattc ctgcagctcg atcggaatca aacgcgtatc gccatgagcc
     1681 atcgtgcgaa acggccctgc gccgtcgtgg gttgcgcagg cgccccaacc gccatgtccg
     1741 ctgtcgtttg ctttaaacac ggcaccggta tccgtccgtt tgccggcaaa gctcagggac
     1801 gagaacgtcc cgaaatgcgc gccggtcacg cgctcgggta aggccggctc caaggctttg
     1861 atgatgcaat cgatcaccgt cggaaaggga atgctgtaga ggcccatcgc cgccgtcggt
     1921 tctgcgctga tgagctttcc ctgcggtaat atcatcttca ccggccgaaa ggttccttcg
     1981 ttggccggtt catctgtcgc gatcaggtat ttgaaagcga cccgcacgca ggttcgcccg
     2041 ccgccgtaga acccggagtt gatgcagcct ttgacctgct ccgacatttc ggagaaatcg
     2101 acggtcatct cgtcgccggc gatcacaact ttgactcgga tcggaatttt ttcgtctcgt
     2161 acgccgtcgt tatcaagata cgcttcgtgg cggtaaacgc cgtccgtaat cgcgcggatt
     2221 ttttcccgag tcgcggcttc gcattgatcg agaatcctgg cgagagcgtc gagaaaaaca
     2281 gaggtgccgt atttatcggc catcgcttgc gtgagatcgc ggccaagcag gcatccgccc
     2341 agctgcgctt cgatgtcgcc gagcaactcc agcggaaagc gcgtgttgtt ttcgattatt
     2401 cgataaactt cctcgatcgg ctcgcccttg gacaaaatct tgatcgagcg cagctgcaag
     2461 ccttccatga aaatatccgt cgaggaatac gagaccgatc cgatcgctcc gccgccaata
     2521 tcgatccagt ggcagttgat ggaaaaaaaa ccgatcaact catcgccgtt ggtcccggcg
     2581 aagaccggcg tgtacatcac ggtattattg agatgttgtc cctgtacggc ggcatgattg
     2641 cagacgatca cgtcgccttt gtgcagattc tctcggccgt aagtctccag gccgtctctg
     2701 accgccatcc ccacagagtc ggcaacaaac acgggcatcc caccggtcga ttgcgccagc
     2761 aattcgccgg ctttgccgac gaatccgaca gcgtagtctt tgtactcgta gatatacgga
     2821 ctaaacgccg tgcgtgtcat gttggcgtcg atctgatcac agatagaaat aattccatga
     2881 cgaatgactt ccaaggtgat ggggtcgatg tctgtacgtt tgtcgagcag ctgtggcata
     2941 ggtgcagatt gcgcttgcgg ttaaaaactt acgcggatat tttcaaggaa ctcctaggtt
     3001 gtcaaggaag gagaatttag cgtaagtaac atgaccgcgg caattcaggc acaacaatcc
     3061 gaggtatcaa gaatggcaga atcttttcgc tgtctttaca cttcgacttt ccagtggtgc
     3121 actc
//
LOCUS       DASULZ010000409         2308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1064400, whole genome shotgun sequence.
ACCESSION   DASULZ010000409 DASULZ010000000
VERSION     DASULZ010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064400"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VFY96_08380"
     CDS             <1..592
                     /locus_tag="VFY96_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5606516.1"
                     /translation="IVIACTGGGSNFAGLAFPFLGLQLRGGRKRRVIAVEPAACPSLT
                     RGKYAYDFGDTAHLTPLVKMHTLGSTFIPPGFHAGGLRYHGMSPLVSHIYELGLIEAR
                     AYHQKACFEAGVEFARCEGIVPAPEANHAVRCAIDEALRCKKEGKQETILFNLSGHGH
                     FDMQAYIAFFEGKLVDQDYDEKELAMALAGLPSVAA"
     gene            638..1711
                     /locus_tag="VFY96_08385"
     CDS             638..1711
                     /locus_tag="VFY96_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX5606517.1"
                     /translation="MIEPYNAVGLIPSFWGIRRREEIKQNIDHLKGLTKAAFWLSNLD
                     IPVRLLAIPEGALMGFNDEVLDVDHADYARTCCIDIPGPETDEIGKLARQWNVFIMAQ
                     AKARHKDWKNRFFNIGFIVDPDGNIILQHYKLTALLPVERSVSPHDIYDWWIKKYGRT
                     LDAFWPVADTKIGRLGIMMAMEGNYPENGRGLAMNGAEVVYRASMPAPFTENDIFEIS
                     NRARALENNMYIVAPNIGSYHLDADHPVGIDAGGGQSMIVDYRGQLVGKQRDTNGSTF
                     VSGVINIEALRHHRESAQVTNWMKDVRAELAQIIYEKPIYPKNRYLKKLPPKHDEYKR
                     DVIDKQVQLMQKRGIWKKSSMKK"
     gene            1900..>2308
                     /locus_tag="VFY96_08390"
     CDS             1900..>2308
                     /locus_tag="VFY96_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262850.1"
                     /note="catalyzes the conversion of salicylyl-CoA to
                     gentisyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HEX5606518.1"
                     /translation="MRAVCLGGGPGGLYFAISLKRRDPAHEVVVIERNRPDDTFGWGV
                     VLSDETLVNLEANDADSAALIRESFVYWDDIAVHYRGQTVRSGGHGFSGIGRMRLLNI
                     LQERARALGVKIQFETEAKDVSAYANYDLIVGAD"
BASE COUNT          509 a          633 c          675 g          491 t
ORIGIN      
        1 cattgtgatc gcctgcaccg gtggcggctc gaactttgcc ggcctggcat tcccgtttct
       61 tggcctgcaa ttgcgcggtg gacgcaagcg gcgcgtgatc gcggtggagc cggcagcgtg
      121 tccgtcgctg acgcggggca agtacgccta cgatttcggc gacacggcgc acctgacgcc
      181 gctggtcaag atgcacacgc tcggctcgac cttcattccg cccggcttcc atgccggcgg
      241 cctgcgctac cacggtatgt cgccgcttgt gtctcacatt tacgaacttg ggctgattga
      301 ggcgcgcgca taccatcaaa aggcctgctt tgaagccgga gtcgaatttg cgcgttgcga
      361 gggtatcgtc ccggcgccgg aagccaatca cgcggtacgc tgcgcgatcg atgaggcttt
      421 acgctgcaag aaagagggca aacaggagac cattctcttc aatctctccg gtcacggcca
      481 ttttgatatg caagcgtaca tcgccttctt cgaaggcaag ctcgttgacc aggactacga
      541 tgaaaaggaa cttgcgatgg ccttggccgg cttgccgagc gtcgcggcgt gaattcctgg
      601 cgattgctca cataagtttc atccgatgag gtaggccatg atcgagccct ataacgccgt
      661 aggcctgatc cccagtttct ggggaattcg ccgacgcgaa gagatcaaac agaacatcga
      721 ccacctcaag gggcttacca aggcggcgtt ctggctttcc aacctcgaca tcccggtgcg
      781 cctgcttgcg atccccgagg gcgcactgat gggtttcaat gatgaagtcc tggacgtaga
      841 ccatgccgat tacgcccgca cttgttgcat cgacattcca ggaccggaaa ccgacgaaat
      901 cggcaagttg gcgcgccagt ggaatgtctt catcatggcg caggccaagg cgcgtcataa
      961 ggactggaag aatcgcttct ttaatatcgg gttcatcgta gatccggacg gcaacattat
     1021 tttgcagcac tacaagctga ccgcgctctt gccggtcgag cgttcggtgt cgccgcatga
     1081 tatctacgat tggtggatca agaaatatgg ccgcacgctc gacgcgttct ggccggtcgc
     1141 cgataccaag attggacgac tcggcattat gatggcgatg gaaggcaact atcccgagaa
     1201 cgggcgcggc cttgcgatga acggtgctga ggtagtttac cgagcgtcga tgccggcccc
     1261 atttacagag aacgatattt tcgagatctc caatcgcgct cgtgcgctgg aaaacaatat
     1321 gtatatcgtc gcgccgaata ttggcagcta ccatctcgat gcggaccacc cggtcggcat
     1381 tgatgccgga ggcggccaat caatgatcgt tgactaccgc gggcagctcg tcggtaagca
     1441 gcgcgacacc aatggttcaa cgttcgtttc aggcgttatc aatatcgaag cgctgcgtca
     1501 ccaccgcgag tcggcgcagg ttaccaattg gatgaaggat gtacgtgcgg aactggcgca
     1561 aatcatctac gagaaaccaa tctatccgaa aaatcggtac ttgaagaaac tgccgccaaa
     1621 gcacgatgag tataagcgtg atgtgatcga caaacaggtc cagcttatgc agaagcgtgg
     1681 gatctggaag aagtcatcaa tgaaaaaata aaactcgcaa gatcggcgcg gctatatcgc
     1741 ctgacccgga tagcgttcgc aggactgcaa gaccaagtaa aagcagcact tttcgcgctc
     1801 ctctcgacag ccgggaggag cgtagattcc tgcggataaa tgctttaagg ttagaaatta
     1861 taaggctata ataaattaat tgaacgatcg gattgggcaa tgcgcgcggt ttgtctcggc
     1921 ggcggccccg gcggcctcta cttcgcgatc tcgctcaagc ggcgggaccc cgcgcacgag
     1981 gttgttgtca tcgagcgcaa tcgcccggac gacacgttcg gctggggtgt ggtgctgtcc
     2041 gacgagaccc tggtcaatct tgaagccaat gatgcggata gcgcggcact catccgcgaa
     2101 tctttcgtct attgggacga catcgcggtg cactatcgcg gtcagacagt gcgctcgggc
     2161 ggtcacggtt tttctggcat cggccgcatg cgtctgctga atatccttca ggagcgggcg
     2221 cgcgctctcg gggtcaagat tcagttcgag acagaggcaa aggacgtttc cgcttatgcg
     2281 aactacgatc tgatcgtcgg tgccgacg
//
LOCUS       DASULZ010000410         5841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_558423, whole genome shotgun sequence.
ACCESSION   DASULZ010000410 DASULZ010000000
VERSION     DASULZ010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558423"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>5841)
                     /locus_tag="VFY96_08395"
     CDS             complement(<1..>5841)
                     /locus_tag="VFY96_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011494422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX5606519.1"
                     /translation="FSIERLEQHGESLAVAQRITTSPRKGRPLLPRVLDNGRVLLQSY
                     RATAQAIREERAITPASEWLVDNYHIVDEQLREIRDDLPPGYYLELPKLAEGHLGGYP
                     RVYGLAWAFVAHTDSRFDPEMLRRFVRAYQHVQPLTIGELWAVAITLRVVLVENLRRL
                     AERIVHEKAARQEADTVVDRLLGLGGSSIEPAARVLSRFEGGPLTTAFAVQLVQRLRD
                     QDPVITPALHWLERQLEAQSTTSDEIVRVEHQQQAAMNVTVRNVITSMRLISAFDWAE
                     FFESVSLVDAILWTNTGFAAPDFVTRDRYRRAIEELSRRSPHDEREVARRAVAHAKDA
                     RAAALEACEPAADRRADPGYYLIANGRVSFEQELGFRMPIKTRLRRAYVTNATSRYLA
                     TIAIVSGLILTLFLFHSGAAGVGVAALLLVALLALVPASDLAIALVNREVTELLGPRV
                     LPRLALRDGVPADLRTLIVVPTLLIAPSQIAEQIERLEVHFLANSDGDLRFALLSDWT
                     DAPQENMPGDDELLAAAVDGIAQLNRRHGQAPDGSERFLLFHRRRRWNESERKWIGWE
                     RKRGKLHELNRLLRGATDTSFVMAGGKASMVSSGVRYVITLDADTRLPRGVACRLVGT
                     MAHPLNRPRFDPRVGRVVEGYALLQPRVTPTLPTDREGSLFQRISSGPAGIDPYASAV
                     SDVYQDLFGEGSYIGKGIYDIDAFEAALAGRVPENALLSHDLFEGIFARAGLVTDIEL
                     FEEFPSNYEVAVVRQHRWARGDWQLLPWIIGDAPAASDAPIRTSIPLIGRWKMLDNLR
                     RTLFAPAAFLTLLAGWTLPFAAPSIWTTFVLACIAIPALLQVLIGAIPRRRGISKRSH
                     ARAVGTDLMLAASHVGLTVTLLAHQAWLMTDAIVRTLTRVYVTRGKLLEWVTAAQAKS
                     GFDLSLRSSYRRMSAAVALAFAAVILVAFQSREVWPIAAPFALVWALSPMIAYWISLP
                     PRAAATQPLSEEDVRTLRLTARRTWRFFETFVGPDDHALPPDNFQEEPTPVVAHRTSP
                     TNIGLYLLSIIAARDFGWIGAVAAIERLEATLGTMKGLERFRGHFYNWYDTRDLRPLD
                     PKYISSVDSGNLAGHLVALGQASREMIDGPLLDPHALMGIGDAILLLRQSARAIVDDR
                     RTQTVTRKHLNEALDALTGALSPVPVSVGDWVLRLTELGARADTMTDIARTLTAERGE
                     TADSELVAWAEALRAVIESHARDLDAVIPWARLVFGKAPINRATIAEHDLVSSTMARF
                     YSSMPTLADTPNWCESAISELTTLRERLMKNGAAQSDGLAGIDATVESLAHSAAACEA
                     LVRRISSLVQLTKAIFEEMEFGFLFDPTRKLFSIGYRVADQSLDPSCYDLLASEARLT
                     SFVAIAKGDVPATHWFHLGRALTPVDRGSALVSWSGSMFEYLMPVLVMRSPSGSLLGQ
                     TCSLVVRRQRKYGTERSVPWGVSESAYNARDLDLTYQYSNFGVPGLGLKRGLSEDVVI
                     APYATALAAMIDPKTAAANFVRLAEAGGSGRYGFYEALDYTATRVPENQTVAVVRAYM
                     AHHQGMSLVALANVLHDGVMRARFHAEPRMQATELLLQERTPRDVLVTRPRVEEVRAV
                     ANIRDLVPPVVRRFDSPHDPTPAVHLLSNGRYSVMVTTAGSGYSRWCDVAVTRWREDR
                     TRDAWGTYVFLRDKSSSQVWSAAYQPSGVEPDTYEVIFSEDHVEIRRRDGAIGTMLEV
                     VVSPEHDAEVRRVSVTNLGARTREIEVTSYAEIVLAPPAADAAHPAFSNLFVQTEFVA
                     DLGALLATRRPRSHGDPQVWMAHVVVAEGETFGDMEYETDRGRFLGRGRGIRTPMSVI
                     DGHPLSKTVGTVLDPIMSLRRCVRLAPGAAARVTFSTLIASSRDDAIALADEYNNPAT
                     FERAATLAWTQAQVQLRHLGIDADEAYLFQELASRILYSDVTLRPPS"
BASE COUNT         1254 a         1795 c         1785 g         1007 t
ORIGIN      
        1 ggagggaggt cgcagtgtaa catccgagta caggatccgg ctggcgagct cctgaaagag
       61 gtacgcctca tcagcgtcaa taccgagatg acgtaactgg acctgagcct gcgtccacgc
      121 cagcgtcgcg gcgcgctcaa atgttgctgg attgttgtac tcgtcggcca gggcgatcgc
      181 gtcgtcgcgc gacgacgcga tcagcgtcga gaacgtgacc cgtgcggccg cccccggcgc
      241 aagccgtaca cagcgcctaa gactcatgat tgggtcaagc acggtgccca ccgtcttaga
      301 gagcgggtgg ccgtcgatga ccgacatcgg cgtgcggatg ccgcgccccc ggccgagaaa
      361 ccggccacgg tcagtttcat attccatatc gccgaacgtt tcgccttcgg ctacgaccac
      421 gtgtgccatc cagacctggg gatcgccgtg cgatcgtggc cggcgcgtcg cgagcagagc
      481 gccaagatcg gcaacaaatt ccgtctgaac aaatagattg gaaaaagcgg gatgagccgc
      541 atccgcagcc ggcggcgcca ggacgatttc ggcgtacgac gtcacctcaa tctctcgggt
      601 tcgtgctccg agattcgtga ccgacacccg gcgcacttcg gcgtcgtgct ccggcgaaac
      661 cacgacttca agcatggtcc cgattgcgcc atcgcgccgc cggatctcga cgtgatcttc
      721 ggaaaaaatg acttcatagg tatccggttc gacgccgctt ggctgatatg cagccgacca
      781 aacctggctg ctggatttat cgcgcaggaa aacgtacgtt ccccaggcgt cacgtgtacg
      841 atcttcccgc catcgtgtaa ccgcaacgtc gcaccagcgg ctgtaaccgg agcccgccgt
      901 agtgaccatc accgaatatc gcccgttgga gaggaggtgg acggcgggcg tcggatcgtg
      961 cggggagtcg aagcggcgaa caaccggtgg aacaagatcg cggatattgg cgacagcccg
     1021 cacctcctcg accctgggcc gggtgaccaa aacgtcacgg ggagtgcgtt cctgcaggag
     1081 caactcagtg gcttgcatgc gcggctcggc gtgaaagcgc gcgcgcatca cgccgtcgtg
     1141 gagcacgttt gcaagcgcca ccaacgacat tccctggtga tgtgccatat aggcacgcac
     1201 gacggccacc gtttggttct cggggacgcg cgtggcggtg taatcgagcg cctcgtaaaa
     1261 accatagcgg ccgctcccgc cggcctcggc aagacgcacg aaattcgccg ccgccgtttt
     1321 gggatcaatc atcgcagcca gggctgtcgc gtatggcgcg atgaccacat cttcactcag
     1381 gccgcgtttg aggccaaggc caggtacgcc aaaattcgag tactggtagg tgagatccag
     1441 atcccgagcg ttgtaagccg attctgaaac accccacgga acactacgtt cggttccata
     1501 ttttctctgg cggcgaacaa caagagaaca agtctggcct aaaagactgc ccgacggaga
     1561 gcgcatgacg agcacgggca tcaagtattc gaacatcgag cccgaccagg agaccagcgc
     1621 ggatccccta tcaactggcg tcaaagcgcg accgaggtga aaccaatggg ttgccggaac
     1681 gtcccccttg gcaatagcaa caaaactggt gagccgcgcc tcggacgcca gcaaatcata
     1741 gcaacttggg tcgaggctct ggtcggcaac gcgataaccg atcgagaaca gtttacgagt
     1801 gggatcgaag agaaagccga attccatctc ctcgaatatg gctttcgtga gctggacaag
     1861 agacgaaatg cgacggacca gcgcctcaca ggctgcggca gaatgcgcaa gactctctac
     1921 cgtcgcatcg atgccggcaa ggccgtcgct ctgtgctgcg ccgttcttca tcaaacgttc
     1981 gcgcagcgtc gtgagttcgc ttatcgcgct ctcgcaccaa ttgggcgtgt cggccaaggt
     2041 cggcatcgac gaataaaagc gcgccatcgt cgacgacact aaatcgtgtt cggcgatcgt
     2101 cgcacgattt atcggagcct tgccaaagac aagtcgcgcc cagggaatca ccgcgtcgag
     2161 atcgcgtgca tgactttcga taaccgctcg cagagcctcg gcccaggcga ctaactcgct
     2221 atcggcggtt tcgcctcgtt ccgcggtcag ggtacgcgca atgtccgtca tcgtatcagc
     2281 gcgggctccg agctcggtaa ggcgcaggac ccaatcaccc acagagacag gaaccggact
     2341 aagagcgccc gtcagcgcat cgagtgcttc attcagatgt ttccgggtga ccgtttgggt
     2401 gcggcggtcg tcaacaatcg ctcgtgccga ttgacgcagg agcagaatgg cgtcaccgat
     2461 ccccatgagc gcatgcgggt cgagcagcgg gccgtcgatc atctctcggg aggcttgtcc
     2521 gagtgcgacg agatggccgg cgaggtttcc actatcgacc gaagatatgt atttaggatc
     2581 gagcggacgg agatcgcgag tatcatacca gttatagaag tggccccgga aacgctcgag
     2641 acccttcata gtgccgagcg tcgcttcgag tcgctcgatc gcggcgactg cgccgatcca
     2701 gccgaaatcg cgcgccgcaa tgatcgagag cagatagagc ccgatgttgg tgggcgaggt
     2761 tcgatgggcc acaaccggag tcggctcttc ctgaaaattg tcgggcggca gcgcgtggtc
     2821 gtccgggccg acaaacgtct cgaagaagcg ccaggtgcgc cgcgcggtca aacgaagcgt
     2881 acgaacgtcc tcctccgaaa gaggttgagt cgccgccgcg cgcggtggca ggctgatcca
     2941 atacgcgatc atcggcgaga gcgcccagac gagagcgaac ggcgccgcaa tcggccagac
     3001 ttcccgcgac tggaacgcaa caaggatgac tgcagcgaac gcgagcgcta ccgcggcgct
     3061 catgcggcga tagctgctgc gaaggctaag gtcgaacccg gattttgctt gcgctgccgt
     3121 cacccactcg aggagctttc cgcgtgtcac atagacgcgc gtgagcgtcc ggacaatcgc
     3181 atcggtcatg agccatgcct ggtgcgccag taatgtcacc gtaagtccga catgcgatgc
     3241 ggcaagcatc agatcggtgc cgaccgcccg cgcatggctg cgtttcgaaa tcccccggcg
     3301 acgcgggatc gcgccgatca atacctgtaa aagggcaggg atagcgatac atgcgagtac
     3361 gaatgtcgtc cagatcgagg gcgcagcgaa cggcagcgtc caaccagcga gcaaagtcag
     3421 aaaggccgcc ggcgcgaaaa gtgtgcggcg gagattatcg agcattttcc accggccgat
     3481 caggggaatc gaggtgcgga tcggagcatc gctcgcggcc ggtgcgtctc cgatgatcca
     3541 cggcaacaac tgccagtcac cccgggccca gcggtgctgg cgcaccacgg cgacctcgta
     3601 attcgaggga aactcctcga ataactcgat atcggtgacg agtcccgccc gcgcaaaaat
     3661 accttcaaag aggtcatggc ttaacaatgc gttctccggc acccgtccgg cgagagcggc
     3721 ctcaaatgcg tcaatgtcat aaatcccttt gccgatgtag gaaccttcgc cgaaaagatc
     3781 ctgatagacg tccgagacgg cagaggcgta cgggtcgatg ccggcaggac cggaggagat
     3841 cctctggaaa agagagccct cgcgatcggt cgggagggtt ggcgtgacac gcggctgcag
     3901 cagagcgtac ccctcaacca cgcgaccgac acgaggatcg aaccgaggcc ggttgagcgg
     3961 atgcgccatt gtgccgacca atcggcaagc gacgcccctc ggtaaccgtg tgtcagcatc
     4021 cagcgtgatc acgtagcgga caccagaaga caccatagat gctttaccgc ccgccatcac
     4081 gaagctagtg tctgtcgcgc cgcgcaacaa gcgattcagt tcatgaagct ttccacgttt
     4141 gcgctcccag ccgatccact tgcgctcgct ctcattccat cggcgccgcc ggtggaaaag
     4201 cagaaatcgc tcgctcccat ctggggcctg tccatggcgg cggttcaatt gcgcgatacc
     4261 gtcgacggcc gccgccaaca gttcgtcgtc tcccggcatg ttttcttgcg gcgcgtctgt
     4321 ccagtcggag aggagcgcga agcgaagatc gccatctgaa ttcgccagga aatgtacttc
     4381 cagacgctca atctgctcgg caatctgaga gggggcgatc aggagagtcg ggacgacaat
     4441 caaggtgcgt agatccgctg gcactccatc gcggagcgcg agcctgggca gcacccgtgg
     4501 gccgagcaat tccgtcacct cacgattcac cagtgcaatt gccagatccg aagccggaac
     4561 gagagcgaga agcgcaacga gcaacagagc cgcaacacca actcctgctg cgcccgaatg
     4621 gaacaggaac aaagtcagga tcagaccgct gacgattgca atggttgcaa ggtaccgaga
     4681 tgttgcattg gtcacgtagg cgcggcgcag ccgggtcttg atcggcatgc gaaagccgag
     4741 ctcctgctcg aaggaaaccc ggccgttcgc gataagatag tagccgggat ccgcgcgtcg
     4801 gtccgcggct ggctcacaag cctccagtgc tgcggcacgc gcatctttcg cgtgagccac
     4861 cgcccgccgg gcaacttcac gctcatcgtg cggcgagcgc cgcgagagtt cttcaatcgc
     4921 acgccgataa cgatctcgcg tcacgaagtc cggcgcggca aaaccggtgt tggtccagag
     4981 tatggcatcg acgagactga cactctcaaa gaattccgcc cagtcgaagg cggaaatcag
     5041 gcgcatactg gtaatcacgt tgcgcaccgt cacgttcatc gctgcctgct gctggtgctc
     5101 aacacggaca atttcgtcag acgtggtgct ctgagcctct aattgccgct cgagccagtg
     5161 aagagcgggc gtgatcacgg gatcctgatc acggagccgc tggacgagct gcacggcgaa
     5221 tgccgtcgtc aatggcccgc cctcgaaccg gctcagaacc cgcgccgcag gctcgattga
     5281 actgccgccg agtccgagga ggcggtcgac caccgtgtcg gcctcctggc gcgccgcctt
     5341 ttcatgcacg atgcgctccg ccaggcgccg gagattctcg accagaacca ctcgcaaagt
     5401 aatcgcaacc gcccatagct cgccgatcgt caatggttgc acgtgctggt aggcgcgcac
     5461 gaagcggcga agcatttccg gatcgaacct gctgtcggta tgcgcgacaa acgcccaggc
     5521 caatccgtag acgcgcggat aacccccgag gtgaccctcc gccagcttgg ggagttccag
     5581 gtaataacca ggcggcaggt catcacggat ctcgcgcaac tgttcgtcga cgatatgata
     5641 attgtcgacc agccattcgg acgcaggtgt gattgcgcgc tcctcacgga ttgcctgggc
     5701 ggttgcacga tacgattgga gcagcacccg gccgttgtcg agcacccgcg gcaacagcgg
     5761 acgacccttg cgcggcgaag tcgtgatgcg ctgagccacg gcgaggcttt ccccgtgctg
     5821 ctcgagacgc tcgatgctga a
//
LOCUS       DASULZ010000411         4595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_558797, whole genome shotgun sequence.
ACCESSION   DASULZ010000411 DASULZ010000000
VERSION     DASULZ010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558797"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1930)
                     /gene="carB"
                     /locus_tag="VFY96_08400"
     CDS             complement(<1..1930)
                     /gene="carB"
                     /locus_tag="VFY96_08400"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX5606520.1"
                     /translation="MPRRDDLKSILLIGAGPIVIGQACEFDYSGTQALKALKEEGYRV
                     ILVNSNPATIMTDPGFADRTYIEPITTDIVEKIIAKEKPDAILPTLGGQTALNLAIDL
                     ADKGILERYGVEMIGAKIGSIKKAEDRDLFKRAMRRIGLDLPRSGYARNMRDALRIQK
                     RLNFPVIIRASRTLGGSGGNFAYGPEEFKEVVQAGLSISPVHEVLIEESVLGWKEFEL
                     EVMRDHKDNVVIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRDAALRVIREIG
                     VDTGGSNIQFAVNPTDGRMVVIEMNPRVSRSSALASKATGFPIAKIAAKLAIGYTLDE
                     IRNDITRETPACFEPTIDYVVVKIPRFTFEKFPQAKDLLTPQMKSVGEAMAIGRTFKE
                     ALQKAMRSLETGSFGFEPKVDLSPAQRSESLEIVRTKLREPNAQRLWYLGDAYRLGMS
                     TAEIYQLSAIDPWFLEKIRQLIACEKEIAGAGSLNRIDARALRQWKQMGFADARIASV
                     IAASEAEVRAARKANAVEAVFNAVDTCGAEFRAFTPYLYSTYEGEDEAKPSNRKKIMI
                     LGGGPNRIGQGIEFDYCCVHAAFALKEAGFETIMVNCNPETVSTDYDTSDKLFFEPLT
                     MEEVMNIVDREKPDGVIVQ"
     gene            complement(1935..3101)
                     /gene="carA"
                     /locus_tag="VFY96_08405"
     CDS             complement(1935..3101)
                     /gene="carA"
                     /locus_tag="VFY96_08405"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX5606521.1"
                     /translation="MKAILALADGRIFEGIGFGAEGEAVGEVVFNTSMTGYQEILTDP
                     SYEGQIVAMTYPEIGNVGVNPEDVESRRPFVRGFIVKNYTPEPSNWRSAQALHEYMKA
                     NGIVGIEGIDTRALVRHLRDYGAQQAIISTVSDNADQLIAKAKGSPGLVGVDLVREVS
                     CAKPYDWDEGRWQLNAGYRRRQPSALAGTGKTEDRLEAPVFSVVAYDFGIKYNILRNL
                     VEARCGVRVVPAATPAEDVLALNPDGIFLSNGPGDPDAVPYAKENVQKLIGKKPIFGI
                     CLGHQIMGLALGGKTYKLKFGHHGGNQPVMDLTTRKVEITAQNHGFAVEADSLKGAVE
                     VTHLNLNDNTVEGLAHRELPIFSVQYHPESSPGPHDADYLFKRFTDLMEKDRGK"
     gene            complement(3105..>4595)
                     /gene="pyrR"
                     /locus_tag="VFY96_08410"
     CDS             complement(3105..>4595)
                     /gene="pyrR"
                     /locus_tag="VFY96_08410"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX5606522.1"
                     /translation="CGDLRNGRTIHSLVFALARFGANVVTLAANGMALPQYVIERLER
                     EYGYGLTPVAASDLSTVVTETDALYLTPKQPHQLALFTQVDEVIQARLSSMATGLRYD
                     AFYMTRKQKERMKDGASGSYPSIGADFLREQRFQDTVVMHPLPRVDELSPEVDKDRRG
                     IYFKQAAYGVPVRMGLLKFLFDRREKGTSARRRQSVIYESPEAIGPQCRNANCVTLNE
                     PVSTRRRFQILSPEEGGAWVVACLYCDHRNKVQYIGNAKSKRYSSFDPSLITSLRDWL
                     KAGELAIFDTIKEAEELGYEPIKSGPQRILLNESEIEQALVDIAGQILRDCQNPDRLL
                     ILGVRSVGAQLAQRLAQEVEKRCQRRPELGEIEIFGAGEELRRVSPADPDAGPLNLKD
                     REVILVDDVLYTGRTVKSALSIIFRSGRPQSVRLAVLIDRGHREVPVRPNYVGKNIPT
                     AENERVRVKLRGLDQENRDQAVLYSIDHAVGTTAPLPVREGTAASR"
BASE COUNT          916 a         1357 c         1264 g         1058 t
ORIGIN      
        1 attgaacgat gaccccgtcg ggcttttccc gatcgacgat gttcatcacc tcttccatcg
       61 tgagcggctc aaaaaacaac ttgtccgagg tgtcatagtc ggtgctgacg gtctccggat
      121 tgcaattgac catgatggtc tcgaagcccg cctctttcag tgcgaaagcg gcatgcacgc
      181 agcagtagtc gaattcgatt ccctgcccga tgcggttcgg gccgccgcca aggatcatga
      241 tctttttacg gttgctgggc ttcgcttcgt cttcgccttc gtaggtggaa tacaggtaag
      301 gcgtaaaggc gcggaactct gcgccgcagg tatccacggc gttgaagacc gcctcgacgg
      361 cgttggcttt ccgcgccgcg cgaacttcgg cttcggaggc agcgatcacg ctggcgatgc
      421 gcgcatcggc gaagcccatc tgcttccact ggcgcaacgc ccttgcgtcg attctgttga
      481 gggatcccgc gccggcaatt tctttctcgc aggcgatgag ctggcgaatc ttttccaaaa
      541 accagggatc gatcgccgag agctgataaa tttctgccgt gctcatgccg agccggtagg
      601 catcgccaag gtaccaaagc cgctgcgcgt tcggttcccg aagcttggtg cgaacgattt
      661 ccaaggattc cgagcgctgc gccggcgaaa gatcaacttt cggctcgaag ccgaaggagc
      721 cggtttcgag cgagcgcatc gctttttgta aagcttcctt gaaagttctg ccgatggcca
      781 tcgcttcgcc caccgatttc atttgcggcg tcagcaaatc cttcgcctgg ggaaattttt
      841 cgaatgtgaa gcgcggaatt tttaccacga cgtagtcaat cgtgggctcg aaacacgccg
      901 gcgtttcgcg cgtaatgtcg ttgcggattt catccagggt atagccgatc gctaattttg
      961 ccgcgatctt tgcgatggga aagccggtgg ccttgctcgc cagcgcggag ctgcgcgata
     1021 cccgtgggtt catttcgatc accaccatgc gtccgtcggt cgggtttacc gcgaactgga
     1081 tattcgatcc gcccgtgtcg acgccgatct cgcggatcac ccggagggca gcgtcacgca
     1141 tgatctggta ttctttatcg gtcaaagtct gggcgggcgc gaccgtgatc gaatcgccgg
     1201 tatgaacgcc catgggatcg aagttttcaa tcgagcagat gatcacgacg ttgtctttgt
     1261 gatcgcgcat cacttcgagc tcgaattcct tccagcccag caccgactct tcgatcaaga
     1321 cttcatggac gggcgagatg ctcaatccgg cttgaacaac ttctttgaat tcctccggac
     1381 cataggcaaa atttccgccg ctgcctccca aagttcgtga ggcgcgaatg atcacgggaa
     1441 aattgagccg tttttgaatc cgcagggcgt cgcgcatgtt gcgcgcgtag ccgctgcgcg
     1501 gcagatcgag accgatgcgg cgcatcgcgc gcttgaagag atcgcggtcc tcggcttttt
     1561 tgatcgagcc gatcttcgcg ccgatcattt cgacgccgta gcgctcgagg atgcccttat
     1621 cggcgagatc gatggcgaga ttcagcgccg tttggccgcc cagagtcgga aggatggcgt
     1681 ctggtttttc tttggcaatg atcttttcga caatgtctgt ggtgatcggc tcgatatacg
     1741 tccggtcggc gaatccggga tcggtcatga tcgtcgccgg attggagtta acgaggatca
     1801 cccgataacc ctcttccttc agggccttca gcgcctgcgt gcccgaatag tcgaactcgc
     1861 aggcttgacc gatcacgatc gggccggcgc cgatgagtag aatcgatttc agatcatctc
     1921 gtctgggcat acgcctactt gccccgatct ttttccatca gatcggtaaa gcgtttgaag
     1981 aggtaatcgg catcgtgcgg ccccggtgac gattcgggat gatactgcac ggaaaagatc
     2041 ggcagctcgc ggtgggccaa tccctcgacc gtgttgtcgt tcaaattcaa gtgcgtcact
     2101 tccacagcgc ccttgagcga atcggcctcc accgcgaagc cgtgattctg ggcggtgatc
     2161 tccactttgc gcgtggtgag atccatgacg ggctgattgc cgccatgatg gccgaattta
     2221 agcttgtaag tcttgccgcc gagggcgagg cccataattt gatggccgag acaaatgccg
     2281 aagatcggtt ttttgccgat caatttttgt acattttcct tggcgtaggg caccgcgtcc
     2341 ggatcacccg ggccgttgga aagaaagatt ccatccgggt tgagcgccag cacatcttct
     2401 gccggtgtcg cagctggaac cacccggaca ccgcaccgag cttcaaccag attgcgcagg
     2461 atgttgtact tgatgccgaa atcgtacgcg accaccgaaa aaaccggcgc ctccaagcga
     2521 tcctctgtct tgcctgtccc cgccagggct gatggctggc gccgtctgta tccggcgtta
     2581 agctgccatc tgccttcgtc ccagtcatac ggtttagcgc aggaaacctc ccgcacaaga
     2641 tcaactccca ccagtccggg tgagcctttg gctttggcaa tgagttggtc ggcattgtcg
     2701 cttacggtcg aaataatcgc ttgctgcgcg ccataatcgc gcaaatggcg caccagcgcg
     2761 cgtgtatcga ttccctcgat accgactatc ccgttcgctt tcatatattc gtgcagcgcc
     2821 tgagctgatc gccagttgct cggctcgggc gtgtaattct tcacgatgaa gccgcgcacg
     2881 aagggccggc gcgactccac atcttccggg ttgacgccga cattgccaat ttccggatac
     2941 gtcatcgcga cgatctgacc ctcatacgac ggatcagtga gaatctcctg atatccggtc
     3001 atgctggtat tgaaaaccac ttcaccgacg gcttcgcctt cggccccgaa accgatgccc
     3061 tcgaagatcc gtccgtcggc caatgccaaa atcgctttca tattctaccg cgatgcggct
     3121 gtcccctcgc ggaccggcag cggcgccgtt gttcccacag catgatcgat cgaatacaac
     3181 acagcctgat cgcgattttc ctgatcgagt ccacgaagct tgacccgcac ccgctcgttt
     3241 tcagcagtgg gaatattctt gccgacataa ttcggtcgca ccggaacttc gcgatgccca
     3301 cgatcgatca agaccgccaa acgtacggac tgcggccggc ccgagcgaaa gatgatgctg
     3361 agcgcgcttt tgactgttct gccggtatag agcacatcgt cgaccagaat cacctcgcgg
     3421 tctttaagat tgagcggacc ggcgtcggga tccgcgggcg agaccctgcg tagctcttcg
     3481 ccggcgccga aaatctcgat ttcccccagc tccgggcgcc tttggcaccg cttttcgacc
     3541 tcttgcgcga ggcgctgtgc caactgcgcg cccacactgc gcacccccaa aatcaacaaa
     3601 cgatccggat tttggcaatc gcgaagaatc tgtccggcga tatcgacgag ggcctgttct
     3661 atttctgact cgttcagcaa aatccgttgt ggaccgcttt tgatcggctc gtaaccgagc
     3721 tcttcggcct ctttgatcgt gtcgaaaatc gccaattcgc ccgccttcaa ccagtcacgg
     3781 agcgatgtta tgagactcgg atcgaaggac gaatatcgtt tcgacttggc attgccgatg
     3841 tactgaacct tgttccgatg atcgcagtaa agacaagcaa caacccaggc gccgccttcc
     3901 tcgggtgaaa gaatctggaa tctgcgccgt gtcgataccg gttcattcaa ggtcacacaa
     3961 ttagcattgc ggcactgcgg gccgatcgcc tccggacttt cgtaaataac cgattggcgc
     4021 ctacgtgcgc ttgtgccctt ttcgcggcgg tcgaataaga atttgagcaa gcccatgcgc
     4081 acgggcacgc cgtacgccgc ttgtttgaaa tagatgccgc ggcgatcctt gtccacttca
     4141 ggggacaact catcgacccg cggcaaagga tgcatcacca cggtgtcctg aaaacgttgt
     4201 tcacgtaaaa aatctgcgcc gatgcttgga tagctgccgc tcgcgccatc tttcatgcgc
     4261 tctttctgct tacgcgtcat gtaaaatgcg tcgtatcgca gcccggttgc catactgctc
     4321 aggcgcgcct gaatcacttc atcgacttgg gtaaagagcg cgagttgatg cggctgcttc
     4381 ggcgtcagat agagcgcgtc ggtttcggtc acgactgtgc tcaggtcact ggcggcgacc
     4441 ggcgttaaac cataaccgta ttcgcgttcg agtctctcga tcacgtactg cggcaacgcc
     4501 atgccgttcg ccgccagcgt aacgacattg gcgccgaaac gggccagagc aaacaccaga
     4561 gaatgaatcg tgcggccatt tcttagatct ccgca
//
LOCUS       DASULZ010000412         3312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1471231, whole genome shotgun sequence.
ACCESSION   DASULZ010000412 DASULZ010000000
VERSION     DASULZ010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471231"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VFY96_08415"
     CDS             complement(<1..471)
                     /locus_tag="VFY96_08415"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEX5606523.1"
                     /translation="MNTKIISIVITMLTLAFASSAESQQTGKVYRIGFLASGSPPDPS
                     ASLSPFAAFRQGLRELGYIESRNILLELRYAKGDHDRLPSLAADLIKIKVDIIVASGL
                     SAVRAARNATDTIPIVMVGAMDPVGFELITSLARPGGNITGLSDSAGRELEGKRL"
     gene            complement(500..1525)
                     /locus_tag="VFY96_08420"
     CDS             complement(500..1525)
                     /locus_tag="VFY96_08420"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606524.1"
                     /translation="MNRFWIFDFGSSIVKTKNKRVLCLVLSTLLFALCVPVQAQQTKK
                     IPRIGFLSPTSDDSRAEAFRQGLRELGYTDGQNIAIEYRWAHGNFDRLPELAIELVRL
                     KLDIIVAVVTQASLAAKKATVTIPIVMIGVADPEGSGLVASLARPGANITGTSSMTAE
                     IVGKQLELLKETFPNISRVTALWNPANPIFQAIQRKEAENTARALGVQLQIQDARGPN
                     EIERAFAALARERTSPLIILNDPVFFSERKRIADLSMNHRLVAVSGTLEYADVGGLMA
                     YGPSFPDMYRRAATYVDKILKGAKPSELPVERPKKFELIVNLKAAQQIGLTIPPHVLA
                     RADRVIK"
     gene            complement(1522..2526)
                     /locus_tag="VFY96_08425"
     CDS             complement(1522..2526)
                     /locus_tag="VFY96_08425"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606525.1"
                     /translation="MRFLGCPLSVGTHIFTLCTILFVLNTPAKAQQSKKIPRIGFVNT
                     EGSPQAPGPQFEAFRQGLRDLGYIEGGNILIDLRYPEENTQRTARFVTELVQLKVDLL
                     VLGAQPAIRAAKEATKTIPIVFVTTQNPVAAGFVNSLARPGGNITGVTRLTRELAGKR
                     LELLKEVLPDTSRVGALVNETQTENDFKPYEAPARALKMQLQLLKVREPNPDFEAALQ
                     AAAKSGATALITVSGSLFNRHIKRFAAVAIGHRLPSMHERTRYVEEGGFMSYSANDTA
                     NYRRAAVYVDKILKGANPADLPVEQPTDFDLAINLKTAKQIRLTIPPNVLARADRVIR
                     "
     gene            complement(2523..>3312)
                     /locus_tag="VFY96_08430"
     CDS             complement(2523..>3312)
                     /locus_tag="VFY96_08430"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606526.1"
                     /translation="TIEWRFAEGKNDRLRELAEDLVRLKVDVIFVINTQAAQAAKKVA
                     GTIPIVFARVSDPTRTGLVASLSRPGGNITGLSNVADELGGKRLELLKETLPHVSQVA
                     VLWNSGNPGVALIVKEMEFASPRLNVQLHSVEMREPKDFLSASETLRKGRFGALFVLD
                     DLLVTSYKREIMDLSQENRMPVISLYAEFVDIGGLMAYGPSIPDMYRRAAMYVDKILK
                     GVKPGELPVEQPTKFELVINLKTAKQIGLTIPPNVLARAGRVIK"
BASE COUNT          754 a          900 c          875 g          783 t
ORIGIN      
        1 cagacgcttc ccttccaact cccgtccggc tgaatcggat agcccagtta tgttcccgcc
       61 cggccgcgcc aggctagtaa tgagctcaaa gccgacagga tccatggcgc cgaccataac
      121 aatgggaatg gtatcagttg cgtttcttgc ggcccggacg gctgacaagc ccgacgccac
      181 gatgatgtct actttgattt tgattagatc cgctgccagg ctcggcagcc gatcatgatc
      241 tcccttggca taacgaagtt ccagtaggat attccgactc tcaatgtagc caagctcacg
      301 gagcccttgc cggaacgcgg caaagggaga taaagacgcg gagggatcag gaggagaacc
      361 agacgctaga aagccaatcc ggtatacttt gcccgtttgc tgcgactcag ctgatgaagc
      421 aaaagctagc gtcaacatcg tgattacgat cgaaatgatt tttgtattca ttctttctcc
      481 gaaatcacaa acgctaaaat tacttgatca ccctatccgc ccgcgccagc acgtggggcg
      541 gaatagtcag gccgatttgc tgcgctgctt tcaaattgac aatgagctcg aatttctttg
      601 gtcgttccac cggcagctct gagggcttag cccctttgag aattttgtct acataggttg
      661 ccgcgcgtct gtacatgtcg gggaagctcg gcccgtaagc catgaggccg ccaacatccg
      721 cgtactccaa cgtgccgctc acggctacga ggcgatggtt catcgacaga tctgcaatgc
      781 gtttgcgctc cgagaagaat accgggtcgt ttaagatgat aagcgggctc gtgcgttctc
      841 gcgctaaagc tgcaaaggcg cgctcgatct cgttggggcc tcgtgcgtcc tgaatctgga
      901 gttgcacgcc caaggcacgt gctgtgtttt cggcctcttt tcgctgaatg gcctgaaaga
      961 ttggattggc cggattccac agcgcagtaa cacgggagat gtttggaaag gtttccttga
     1021 gcagctctaa ctgtttcccc acgatctccg cggtcatcga ggacgttcca gttatattcg
     1081 ctcccggccg cgccaggctt gcaacgagac cggagccttc aggatccgca acaccgatca
     1141 taacaatggg tatcgtcacc gtagctttct tagcggccaa cgaagcttgg gttacgacgg
     1201 caacaatgat gtcgagcttg agccggacaa gttcgatagc aagttcaggg agccggtcaa
     1261 aatttccatg tgcccatcga tactcaatgg cgatgttttg tccgtctgtg tagcccagct
     1321 cacggagtcc ttgtctaaag gcttcagcgc gagaatcgtc ggaagtagga cttagaaacc
     1381 cgatccgtgg aattttcttc gtctgctgcg cctgaaccgg aacgcaaagc gcgaaaagca
     1441 gagtgctaag cacaaggcaa agaactcgtt tgttcttggt tttcacaatc gaagacccaa
     1501 aatcgaaaat ccaaaatcgg ttcatcggat cactcgatct gctctcgcga gcacattggg
     1561 cggaatggtt aatctaatct gcttggcggt tttgagattg atcgcaagat cgaagtccgt
     1621 cggctgctca actggcagat ccgcagggtt cgcgccttta agaatcttat ctacatagac
     1681 agcggctcgc cggtagttgg cagtatcatt ggccgaatag gacatgaaac caccttcttc
     1741 gacgtaccga gtcctctcgt gcattgaagg tagacggtgt cctatggcaa cggcagcaaa
     1801 gcgctttatg tgacgattga acagcgaacc actgacggtg atgagggcag tggctcccga
     1861 cttcgctgca gcttgcaatg ccgcttcgaa atcaggattc ggctctcgca ctttgagcag
     1921 ttgaagctgc atcttcaatg cgcgtgccgg cgcctcatac ggtttgaaat cattttccgt
     1981 ctgtgtctca ttcacgagcg ctccgacgcg cgatgtatcg ggaagcactt ccttgagcaa
     2041 ttcgagacgt ttcccggcca gctcgcgggt gagtctggta acccccgtaa tatttccgcc
     2101 ggggcgcgcc aagctattga caaagccggc cgcgactgga ttctgggtcg tgacgaatac
     2161 aataggaatc gtcttggtcg cttctttggc tgcgcggatc gcaggttgag ctccaaggac
     2221 gaggagatcg acttttagtt gcacgagttc ggttacaaat ctcgcagtgc gctgtgtgtt
     2281 ttcctcaggg tagcgaaggt caatgaggat atttccgccc tcgatataac cgaggtcccg
     2341 cagtccctga cggaatgcct caaattgagg cccgggagct tggggactcc cctcagtgtt
     2401 gacgaatcct attcgcggaa ttttcttcga ctgctgcgcc ttagccggag tattaagaac
     2461 aaacaatatg gtgcatagcg taaaaatatg ggtgcctact gacagcggac aaccgagaaa
     2521 tctcacttga tcacccgacc tgccctcgcc agcacattcg gcggaatcgt cagtccgatc
     2581 tgtttggcag ttttgaggtt aataacgagt tcaaactttg tcggctgttc cactggtaac
     2641 tcgcccggct ttacaccctt gagaattttg tccacgtaca tcgcggctcg ccggtacatg
     2701 tccgggatac tcggtccgta ggccatcagg ccaccgatgt cgacgaattc cgcgtacagg
     2761 gaaatcacag gcatccgatt ttcttgggac aaatccatga tttcgcgctt gtaggaggtc
     2821 actaacaagt catctaaaac gaaaagcgcg ccgaagcgtc cctttcttag ggtttcagag
     2881 gcgctgagaa agtccttggg ctctcgcatc tccacgctgt gaagctgcac gtttagccga
     2941 ggactggcaa actccatctc cttcacgata agtgccacgc cgggatttcc agaattccaa
     3001 agcaccgcaa cttgcgagac gtggggaagc gtctccttca ataactcaag ccgcttgccg
     3061 cccaactcat cagcgacatt tgacaatccc gtgatgttgc cacccggccg tgaaagactc
     3121 gcgacgaggc ccgttcgagt cggatccgag actctggcga agacgatggg aattgttccc
     3181 gcgaccttct tggcagcctg cgccgcctgg gtattaatta caaaaataac gtctaccttg
     3241 aggcggacga ggtcttccgc aagctctcga agtcgatcat ttttgccctc agcaaatcgc
     3301 cactcgatag tg
//
LOCUS       DASULZ010000413         4129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_354862, whole genome shotgun sequence.
ACCESSION   DASULZ010000413 DASULZ010000000
VERSION     DASULZ010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354862"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="VFY96_08435"
     CDS             <1..958
                     /locus_tag="VFY96_08435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HEX5606527.1"
                     /translation="TIDEEWCEFLAKENFYIGLSLDGPRELHDHYRVTKGQKPTHKQV
                     VQAFHMLRKAKVHVDLLCVVHDVNVKHSTQVYRYLKEIGGQYLQFLPLVEKTGDPAHP
                     VHKRSVPAAAYGTFLNVIFDEWVRHDIGRIFIQLFDESVRPFLGMEHALCIYRETCGD
                     VPVVEHNGDFYSCDHYVLPEYKIGNIHETPIAEMVEDPRQRQFGQNKWASLPKYCREC
                     EVLSMCNGGCPKDRIINTPDGEAGLNYLCAGLKAFFTHSKPYFQKFAELVEAGEPGEK
                     LMEIVRTADSKASVRQAGRNDPCPCGSGKKYKRCCGGTRPSA"
     gene            1236..2126
                     /locus_tag="VFY96_08440"
     CDS             1236..2126
                     /locus_tag="VFY96_08440"
                     /inference="COORDINATES: protein motif:HMM:NF040043.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HEX5606528.1"
                     /translation="MTLYDDMMEGAIDVHCHIDFEFSAKERKREPEFLWLPKAEALGM
                     RGVVLKSHWWPTINVVPYILATTPTKVALWSSVALNSTAGGPNPCIIEACAELGGKMV
                     FLPTWSARNDVERNGFSSRIKSYYKRHASLENPNYYFLDDKGKLIPRGFEIIEACKAH
                     DLTLGMGHVSWQESLAFIEAARDMGYSHRIVVNHVQSHMIAMPVDAMKRAGEMGAFLE
                     ACWNALAPGRMDSPELVKQFRDVGLRQIIASTDYFRPYSPNPAELFRMFLGMLHEGGL
                     SQGEVNQVASINPARVMGVQ"
     gene            complement(2144..2473)
                     /locus_tag="VFY96_08445"
     CDS             complement(2144..2473)
                     /locus_tag="VFY96_08445"
                     /inference="COORDINATES: protein motif:HMM:NF024485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX5606529.1"
                     /translation="MSSVAKLRIKRGSFGESNRYDDFEVPYEDGMSLLDALRWIRIYR
                     DSTLGIRYSCINANACKTCVALVDGQVEYTCMVKLKPALITVEPLPKRQLVRDLVTDI
                     VSDDEKL"
     gene            complement(2470..>4129)
                     /locus_tag="VFY96_08450"
     CDS             complement(2470..>4129)
                     /locus_tag="VFY96_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX5606530.1"
                     /translation="AAIAAARAGATVLLVDKGQIGRGGATVMAQMTVAAALAEEEPDS
                     VALHLEDTLKAGRGLCNEELAALVCSEGPKRIHEMEEWGTRWAREGERIKQVIAPGHS
                     VKRCCYVDFLNTGPAVAATLRRKIIEFPKIRRVSNVAVVEILTLDGRVMGAIALDLAK
                     NQLFTIQAPAVIISAGGLTKLFARNSASANMGGEAYALGLWAGAELIDMEFVQFFPIG
                     HLAPRLVGMDPIMWDPFRYKLGGKLLNGQFEEFIDRYGSTDFGQYNATRDIAAYAILK
                     EVEAGRGTPHGGAYLDFRHIAEKELRAAFGPVIDRLLANGIDLSRMPVEVGPMAHYHM
                     GGIRVDANMRTRIDGLYAAGEAVGGSNGANRLSGNAITEAFVFGERAGSAVADYVNTS
                     HPDWNEKLAEESLVRLEPFGRSSDDNGIAPISLHIELQQLMWDLAGPFRTGDKLTRAQ
                     ARIADIKNSELGHLHLGREREFNLDVQDGFELRAMMTAAEAVVHAALGRAESRGAHQR
                     EDFPLADYQLIKNQVLELRDGEVVCRWAQPVRLDRSQLDKPLGP"
BASE COUNT          899 a         1208 c         1139 g          883 t
ORIGIN      
        1 gacgattgat gaagagtggt gtgagtttct cgccaaagaa aatttttata tcgggttgag
       61 tctcgacggc ccgcgcgagc tgcacgatca ctaccgcgtc accaagggtc agaagccgac
      121 ccacaaacag gtcgtgcagg cttttcacat gctccgaaaa gccaaggtgc atgtcgatct
      181 actatgcgtc gtccacgacg tcaatgtgaa acactcgacc caggtttacc gttacctcaa
      241 agaaatcggc gggcaatatc tgcagttttt acccttggtc gagaagaccg gcgatccggc
      301 tcatccggtg cacaagcgtt ctgtcccggc ggcggcttac ggtacttttc tgaacgtcat
      361 cttcgacgag tgggtgcgcc acgatatcgg caggatcttc attcagttgt tcgatgagtc
      421 ggtgcgacct tttctcggca tggagcatgc gctctgcatt taccgcgaaa cctgcggcga
      481 cgtaccggtg gtggagcaca acggcgattt ttattcctgc gaccattacg tcctgccgga
      541 atataagatc ggcaatattc atgaaacccc gatcgccgaa atggtcgagg acccgcgcca
      601 gcggcaattt gggcaaaata aatgggcgag cctgcctaag tattgtcgcg aatgcgaagt
      661 gctcagcatg tgtaacggtg gctgtcccaa ggaccgtatc atcaacacac ccgacggcga
      721 ggccgggctg aattacctgt gcgcgggctt gaaagcattc ttcacgcaca gcaagcccta
      781 cttccaaaaa ttcgccgagc ttgtggaagc cggcgagccc ggcgaaaagc tgatggaaat
      841 cgtccgcacc gccgactcga aagcctccgt tcgtcaagcc ggccggaacg acccctgtcc
      901 ctgcggcagc ggaaaaaagt acaaacgctg ctgcggcggc accagaccga gcgcgtaaga
      961 cttaacgctc accacaacat tgcgaaattg agcggggttc taccgtagct tgatctttcc
     1021 ccggtaagta ggccgtgtcg caatctgcaa atcacccaaa tagcattctg agccgaaggc
     1081 gaagaatctc gtgtgcctgc caatcacacg aatcagatcc ttcggctgcg cttcagcatg
     1141 agattgcgac aagcctccac ggcgtggagg aaagagtcgg gatttttcgc gatgttcgtg
     1201 atgaagccat ttatttctga ggtgagggaa attgcatgac tctgtacgac gacatgatgg
     1261 aaggcgcgat cgatgttcat tgccacatcg atttcgagtt ttccgccaaa gaacgtaaac
     1321 gcgagccgga gtttctctgg cttccaaagg ccgaggcgtt ggggatgcgc ggcgtcgtgc
     1381 tcaagtccca ctggtggccg accatcaatg tcgttcctta cattcttgcc accacgccca
     1441 cgaaggttgc cctgtggtcg agtgtcgccc tcaacagcac cgccggcggt cccaatcctt
     1501 gcatcatcga ggcctgcgcc gagttgggcg gcaaaatggt atttcttccg acctggagcg
     1561 cgcgcaacga tgtggaacgc aacggtttca gcagccgaat caaatcctac tacaagcgcc
     1621 atgcgtcgct cgaaaatccg aattattatt tcctcgatga caaaggaaaa ctaatcccgc
     1681 gcggttttga aatcatcgaa gcgtgcaagg cgcatgacct gacgcttggc atggggcatg
     1741 tttcctggca ggaatcgctg gcgttcatcg aagccgcgcg cgatatggga tatagccaca
     1801 ggattgtcgt caatcacgtg cagagccata tgatcgcgat gcccgtcgac gccatgaagc
     1861 gcgccgggga aatgggcgcg ttcctcgaag cctgctggaa tgcccttgcg cccggccgaa
     1921 tggactcgcc cgagctcgtc aaacagtttc gcgacgtcgg cttaagacag attattgcaa
     1981 gcacggatta ttttcggccc tacagtccga atccggccga gctctttaga atgtttttgg
     2041 gcatgctaca cgaaggcggt ttgagccaag gcgaagtgaa tcaggtcgcg tccattaatc
     2101 cagcacgcgt aatgggagtg caatgaatca gcggtttggc ccgctaaagc ttttcgtcgt
     2161 ctgaaacgat atccgtgacc aagtctctga ccaactgccg cttcggcagc ggttcgactg
     2221 tgatcagcgc gggttttagt ttgaccatgc aggtgtattc cacttggccg tcgacaagcg
     2281 ccacgcaggt cttgcaggcg ttagcattga tgcagctata gcgaatgccg agcgtgctgt
     2341 cccgataaat gcggatccag cgcagcgcat cgagcagcga catgccgtcc tcatacggca
     2401 cttcaaagtc atcgtagcga tttgattcac cgaaggagcc ccgcttgatg cgaagtttcg
     2461 ctactgagct catggtccaa gcggcttgtc gagctggctg cgatcaaggc gcaccggctg
     2521 cgcccaccgg cagacaactt cgccgtctct tagctccagg acttgattct tgatcagctg
     2581 ataatcggca agcggaaaat cctcccgctg atgcgcgccg cgactctcag ctcgtccgag
     2641 cgccgcatgc accaccgctt ccgcggctgt catcatcgcg cgcaactcga atccgtcttg
     2701 cacgtcgagg ttgaactcac gctcgcggcc caagtgcaaa tggcccaact ccgagttttt
     2761 gatgtcggcg atgcgtgctt gtgcgcgagt caatttgtcg ccggttcgaa acggccccgc
     2821 gagatcccac atcagttgtt gcagttcgat gtgaagcgaa atcggcgcga taccgttgtc
     2881 atcggaactt cgaccaaagg gctccaatcg aacgagactt tcttcagcaa gcttttcatt
     2941 ccagtcgggg tgcgaagtgt tgacgtaatc agcgactgcc gaaccggcac gttccccgaa
     3001 cacgaacgct tcggtaatgg cgttgccgga cagccggttc gccccgttcg aaccacctac
     3061 cgcctcgccg gcggcatata gcccgtcgat tcgcgtccgc atattagcat cgacccgaat
     3121 accgcccatg tggtaatgcg ccatcgggcc gacttccact ggcattcggc tgagatcgat
     3181 cccgttcgcg agcaggcggt caatcacggg accaaaggcc gcgcgcagtt ccttctccgc
     3241 aatatgccgg aaatccagat aagcaccgcc gtgcggcgtg cctcgaccgg cttcgacctc
     3301 ttttaggatt gcatacgcgg cgatgtcgcg cgtcgcattg tattgaccga agtcggtgct
     3361 gccgtagcga tcgatgaact cttcgaattg cccgttgagc agtttgccgc cgagtttata
     3421 gcgaaaagga tcccacataa tgggatccat gccgacgagc cgcggcgcaa gatgcccgat
     3481 cggaaaaaat tgcacaaact ccatgtcaat gagctcggcg ccggcccaca gccccaacgc
     3541 atacgcctcg ccgcccatat ttgccgaagc gctgttgcgc gcaaaaagct tggtgagacc
     3601 acccgctgaa atgatgacgg ctggcgcctg gatcgtgaat agctgatttt ttgcgagatc
     3661 aagcgcgatc gcgcccatga cgcggccgtc aagagttaaa atttcgacga ctgcgacatt
     3721 gctgacccgc cgaatctttg gaaattctat aatttttcgc cttagagtcg cggcgacggc
     3781 ggggccggtg ttgagaaagt cgacgtaaca gcaacgtttg acgctgtgtc cgggtgctat
     3841 tacttgtttg attcgctcgc cttcccgcgc ccagcgagtg ccccattcct ccatttcatg
     3901 gatgcgcttc gggccctccg agcacaccag cgcggcgagc tcttcgttgc agagaccgcg
     3961 tccagccttg agcgtgtcct cgaggtgaag cgcaacagaa tccggctctt cttccgccaa
     4021 agcggctgcc acagtcattt gcgccatgac ggttgcaccg ccccggccga tctggccctt
     4081 atcgaccagc aacacggtcg cgccagcccg cgcagcagca atggccgca
//
LOCUS       DASULZ010000414         2043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_559074, whole genome shotgun sequence.
ACCESSION   DASULZ010000414 DASULZ010000000
VERSION     DASULZ010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559074"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1609)
                     /locus_tag="VFY96_08455"
     CDS             complement(53..1609)
                     /locus_tag="VFY96_08455"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5606531.1"
                     /translation="MNSRFIQLICVLLMATFAASCSKHEPSKPAEKTLYTCSMHPQVV
                     QDHPGDCPICGMKLVPVRKDSASAGEDRKKTGGKVKYYKSTMLLGEISQTPRKDSMGM
                     DMVAVYEGEEEANAISVDPATVQKMGVRTAVVKKGPLRRVIRTVGAIDYNETALADVT
                     TKFRGWIEKLYVDSTGKQVRKGEPLFDIYSPELYSAQNEYVLGLGQGAGGLKASARQK
                     LKLFDISDDQIAELEKTRQPQRTLRVNAPIDGIVVEKMAVQGQMVEAGMKLYRLADLG
                     IVWVQSQIYEQDLALLKLGQEAEVSLSYMPDRKFRGRVTYIYPTVDEKTRTARVRMEF
                     HNPGLFLKPGMFATVELNAEIEPSALLVPDSAVLRSGEKNTVFVALDNGHFEPREVIL
                     GPRGDNNTYEVLSGLKEDQHVVTSGQFMLDSESQLREAIQKMLNPNASPTLAQDHAAH
                     RPPPKEPTMPAVESTPTAYVCPMPEHATIKYNHPGKCPICGMTLIPAPSDNVDTPSPT
                     PSTTPHKHEP"
     gene            complement(1606..>2043)
                     /locus_tag="VFY96_08460"
     CDS             complement(1606..>2043)
                     /locus_tag="VFY96_08460"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX5606532.1"
                     /translation="SAAQAVSEVDVGVSIPVPWLNWKKYSAGVQEARKTVEEAERQFD
                     AARVETLGLVRDQLKKIETSAKQYELYHDNILPLARQTIDASRSAYEASTGGFLELIT
                     ARRTLQDAESAALNRLVEYDVAIAELDAIIGRARPLVEEGGKR"
BASE COUNT          403 a          584 c          534 g          522 t
ORIGIN      
        1 tcacgaactt gtttcgcaac gaaaaatcta tgatcgcgcg cagcatggat ggttatggct
       61 cgtgcttgtg cggcgtcgta ctcggcgtcg gtgaaggagt gtccacatta tcactgggcg
      121 cgggaataag ggtcattccg caaattgggc atttgcccgg gtggttatat ttgatcgtcg
      181 catgctctgg catcggacag acgtacgccg tcggggttga ttctaccgct ggcattgttg
      241 gttcctttgg cggcggtcga tgagcagcat gatcctgggc aagggtcggg ctcgcattcg
      301 gattcaacat tttttgaatg gcttcccgga gctggctttc cgaatcgagc atgaactggc
      361 cggaggttac gacgtgctgg tcttccttga gaccactcag gacttcataa gtattattat
      421 cgccccgggg tccgagtatc acttcacgcg gttcgaagtg gccattgtcc aaggcgacaa
      481 agaccgtgtt cttttcaccg cttcgtagga cggctgaatc gggcacgaga agagcagacg
      541 gttcgatttc ggcattcagt tctacggtgg cgaacattcc gggtttcaag aacaggccgg
      601 gattatgaaa ttccatccgc acacgcgctg ttcgcgtttt ttcatccacg gtcgggtaaa
      661 tataagtaac acggccgcgg aatttgcggt ccggcatgta ggaaagactg acttccgctt
      721 cctgcccgag tttgagcagc gccaagtcct gttcgtagat ctgggattgc acccaaacga
      781 tgccgagatc ggccagccgg tacagcttca ttcctgcttc gaccatctgc ccttgcaccg
      841 ccattttctc gaccacgatt ccgtcgatcg gagcattgac gcgcaaggtt cgttgcggct
      901 ggcgtgtctt ctccagctcg gcgatttgat cgtcggaaat atcaaacaac ttcagcttct
      961 gtcgcgcact tgctttgagt ccaccagcgc cctggccgag gccaagcaca tactcatttt
     1021 gcgcgctgta aagttcaggc gaataaatat cgaataacgg ctcgcctttg cgcacctgtt
     1081 tgccggttga atcgacatag agcttctcga tccagccgcg aaacttcgtg gtcacgtccg
     1141 caagcgccgt ctcattgtag tcaatcgcgc cgactgtccg gatcacgcgt cgcagggggc
     1201 cttttttcac cacggctgtg cgcaccccca ttttttgcac cgtagctgga tcgacactga
     1261 tcgcattcgc ctcttcctcg ccttcgtaca cagcgaccat gtccattccc atgctgtcct
     1321 ttcgcggcgt ctggctgatc tcgcctaaca acatcgtaga tttgtaatat ttgactttcc
     1381 cgccggtctt tttgcgatct tctccagcag acgcgctatc cttgcgcacc ggcacaagct
     1441 tcatgccgca aatcggacaa tcccccggat ggtcctgcac tacctgcggg tgcatgctgc
     1501 atgtgtagag cgttttttct gcaggcttcg acggttcgtg tttcgaacag gatgccgcga
     1561 acgtcgccat caacaacacg caaataagtt ggataaaacg tgagttcaac gtttacctcc
     1621 ctcttcaacg agcggccttg ctcgtccgat gattgcgtcc aattccgcga tcgccacgtc
     1681 gtattcaacg agacgattta gcgcagcaga ttctgcgtcc tgcaaagtgc ggcgggccgt
     1741 gatcagctcc agaaacccgc ccgtgctggc ttcataggcg ctgcgactcg catcgatagt
     1801 ttgccgcgcg agaggcagaa tattgtcgtg gtaaagctcg tattgttttg ccgacgtctc
     1861 gatctttttc agctgatcac gcaccagacc aagtgtctcc acgcgcgcgg catcaaattg
     1921 tcgttcggct tcttcaacgg ttttgcgagc ttcttgcact cccgctgaat actttttcca
     1981 gttcagccac ggaaccggaa tggaaacgcc aacatccact tcactgactg cctgggccgc
     2041 cga
//
LOCUS       DASULZ010000415         3506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_559104, whole genome shotgun sequence.
ACCESSION   DASULZ010000415 DASULZ010000000
VERSION     DASULZ010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559104"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            334..1296
                     /locus_tag="VFY96_08465"
     CDS             334..1296
                     /locus_tag="VFY96_08465"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015183527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5606533.1"
                     /translation="MAKKIPDAEMPYRTLGSTKERVSAVGLGGWHLGLKKLDEPLSIR
                     IVREAIDRGINFMDNCWDYNDGASETRMGKALRDGYRSKVFLMTKIDGRSKKEAARQL
                     EESLRRLQTDCIDLVQHHEILRYEDPHRIFDEDGANAALGEARKAGKLRYIGFTGHKD
                     PHIHLYMLEVAEQHGFKFDTVQMPLNVMDAHYRSFEKLALPELVKQKIGVLGMKSMAN
                     GIILKSNTVTAIECLHYALNLPTSVVITGVDSMKILDQAFEAARTFRPLSKAQVKSLL
                     AKTKKSASRGEFELFKTTSIFDGTASNPSWLGDEPERVQRVMPA"
     gene            2135..3097
                     /locus_tag="VFY96_08470"
     CDS             2135..3097
                     /locus_tag="VFY96_08470"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5606534.1"
                     /translation="MPFNHNDPETNQTIFSRKGFIFPLIVLLVLVVLSQSVSAQAKSD
                     EVKSIRLGLISDNDPSAVQERFREFVRLVARRLSARSENDAKVLIAPTIFDMAKLLEQ
                     QRVDFYMESPYPTYLINYVHGVGKTLLRRWKGGQAEYQGLIITKRDAGIKQLSDLRGK
                     SIVFEDPGSTSGYLLPKIFLSRKGFKLVEKRDYDPFASPDEIVYFFARSQRRLLQWLL
                     TGQAAAAAMSDEDYAGLDKKEKAKITVLNQTEKLARHLVSVRSDFPPLWMNSLEKVLL
                     SMHEDEDGKRVLYRIDQTTKFDRIPGGEEALRKKLLDVFAPDRR"
     gene            3214..>3506
                     /locus_tag="VFY96_08475"
     CDS             3214..>3506
                     /locus_tag="VFY96_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606535.1"
                     /translation="MKRKRLAHNVFAIEAFIALSLFVFTGAAFSQADFYKGKTIKIIR
                     GGGPGGSGEFQTRALVKFLEKYIPGKPTITVEFIEGAAGRKAANIIYSSSRADG"
BASE COUNT          887 a          873 c          973 g          773 t
ORIGIN      
        1 ggcggagatc gccatttacg cgcagttttt ggcacccgat gaaaccgccg aagaccttat
       61 cgtgagaatg gaagatctct ggcgggtcgc aaagcaggct tgagtctgaa cgatacgggt
      121 gggggggccc aggcattccg acgatgtttc atcgttattg cgatgaacgg ttgcgccgga
      181 ccagatccgg aaccggactc aagaggccgc agccctggac cccttacgga agcgtcttgt
      241 agacacacgg ctgggtgaaa tactaaacca aagagccacc agccgagagc gagacagtgg
      301 atttaaaaag gcgattcaga acgggagaag atcatggcga aaaaaatacc agacgctgaa
      361 atgccgtacc gcaccctcgg cagcaccaaa gaacgggtat cggccgttgg tcttggaggc
      421 tggcacctcg gtctcaagaa gttagatgaa ccgctgagca tccggatcgt tcgcgaagcg
      481 atcgatcgcg gcatcaactt tatggacaac tgctgggact ataacgatgg cgccagtgag
      541 acccggatgg gcaaagcgtt gcgcgacggc taccggagca aagtattcct gatgaccaaa
      601 atcgacggcc gctcgaaaaa ggaagccgcg cggcaactgg aagagtcgct gcgtcgcctg
      661 cagactgact gcatcgatct ggtccagcac cacgagatac tccgctacga ggatccgcat
      721 cgcatcttcg atgaagacgg cgccaatgcc gcgctcggcg aggcgcgcaa agcgggaaag
      781 ctccgctaca tcggctttac cggtcacaag gacccgcaca tccacctcta tatgctcgag
      841 gtggccgagc aacacggctt caaattcgat acggtacaga tgccgctcaa cgttatggac
      901 gcgcattacc ggagctttga aaaactggca ctcccggagc tcgtgaagca aaagatcggc
      961 gtgctcggga tgaaaagtat ggctaatggg attattctga aatcgaatac agtgaccgca
     1021 atagagtgcc ttcactatgc gctgaattta ccgacatcgg tggtgatcac cggtgttgat
     1081 agtatgaaaa ttctcgatca ggctttcgag gcggcacgta cgtttcgccc cctgagcaag
     1141 gcccaagtga aatcgttgct ggcgaagact aaaaaatccg cgtcgcgcgg cgaattcgag
     1201 ttgttcaaga caacgtcgat tttcgacggc acggcttcca atccttcgtg gctcggcgat
     1261 gagccagagc gagtgcagcg ggtgatgcca gcgtagaaaa ggcgtgggca gggcggacgc
     1321 tgaacagagt tggcacttgg ctcggaccag ccctgttgag acctcgccgg gctatctgtc
     1381 accgcaccgt cagtctggga atcggttccg aagagtttaa ccatttaacc aggtctgttc
     1441 agtgggaagt cgtcgacctg gatggatgga tcggctgaat ggtctatggg tgggtttcgg
     1501 ctcgatcagt catgtaccat cagaattccg agaataattc cagctacgat tccagcagaa
     1561 aaaatcaaaa gcatctcgtt catcaggatt gccttcgagt agaatttctt gtcgctcagt
     1621 tacaggtatt acagaccaga tgactatcgc ggcgatttgt tttgtaggaa agcaaaatgc
     1681 acaccaacaa agcaccatct ggaaacgaat agttctcagc cgttattttg gcattttggt
     1741 cggcgggtcg tccaaaaatg tgaatggttc atcaatagcg gtacaggtaa ggtacgcaaa
     1801 caggcacata tgtagaatat ttcaaaagca aaaacaccct cggtctttgg ccaaagctcc
     1861 cgaggtcgat cgatttggaa ctctgcccct tgcctactac gctgttgctt cttgcctcac
     1921 gcgcgttatt tattgcctcg tatggtttgg cgattttatt gcctactgcc tacgctattg
     1981 cctcgaagta gcgaggtagc acgtcggggt gtcgctcaag aacgaaatca gatatgacga
     2041 gcttgctgta tcagcaacgt aacttgacaa gctgtcgcat tgatgtctga agatttaact
     2101 cgaacaggca acaggcagct gtggaaattc tgcaatgccg ttcaatcata acgatccaga
     2161 aacgaaccag acgatctttt ctagaaaagg atttatcttt cctttgatcg tgctcctggt
     2221 gctggtggtg ttatcccaaa gtgtcagcgc gcaagccaag agtgacgaag tcaaatcgat
     2281 tcggttgggg ctcatctcgg acaacgatcc gtccgccgtt caagaacgtt tccgtgagtt
     2341 cgttcgtttg gtggcgcggc gtctgtcggc ccgctcggag aacgacgcca aggtgctgat
     2401 cgcaccaacg atattcgata tggccaagct gctcgagcag cagcgggtcg atttctatat
     2461 ggagagtccc tatccaacct acctcatcaa ctatgtgcac ggtgtcggaa aaactctgct
     2521 gcgccggtgg aagggaggtc aagccgaata tcaagggctg atcattacta aacgggacgc
     2581 cggtataaaa cagctatccg atctgcgcgg taagagcatt gtcttcgaag atccgggttc
     2641 aacttcgggc tatctgctgc ccaagatctt tctgtcgcgc aagggtttta agctcgtcga
     2701 aaagcgcgac tacgatcctt ttgcatcacc ggacgagatc gtttactttt tcgcccgctc
     2761 gcagcgcaga ttgcttcagt ggcttttgac cgggcaggcc gccgccgcag cgatgagtga
     2821 cgaggactac gccgggctgg ataaaaaaga gaaggccaag attaccgtac tgaaccaaac
     2881 agaaaagctg gcgcgtcatc tagtttcggt ccgctcagat tttccgccgc tgtggatgaa
     2941 tagtttggag aaagttctgc tgtcgatgca tgaagacgaa gatggcaagc gagtcttgta
     3001 caggattgat cagaccacta agtttgaccg aataccggga ggcgaagaag cactgcgcaa
     3061 aaaactgctg gatgtttttg cgccggaccg acgataaact aaggcttgtt gaattgccgg
     3121 gctccggatg gagattaagg tcgcggcctg catccgggat cgattgcgct atgctatgga
     3181 tcaaacagtg cggctaaaga tgaggacgtc tatatgaaac gcaagagatt ggcgcataac
     3241 gtattcgcga ttgaagcttt tatcgcacta tcgctgttcg tattcacggg cgcagcgttt
     3301 tcccaagcag acttttacaa agggaaaacg atcaagatta ttcgtggcgg cgggcccggc
     3361 ggctccggcg agttccaaac ccgcgcgctg gtgaaatttc tcgaaaaata tattccagga
     3421 aaacccacca taaccgtgga attcattgaa ggtgctgccg gccgaaaggc agccaacatc
     3481 atttatagct ccagccgggc cgacgg
//
LOCUS       DASULZ010000416         2465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_254032, whole genome shotgun sequence.
ACCESSION   DASULZ010000416 DASULZ010000000
VERSION     DASULZ010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254032"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..604
                     /locus_tag="VFY96_08480"
     CDS             203..604
                     /locus_tag="VFY96_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606536.1"
                     /translation="MLRPIEKLSLGVIIIAVFYAFGLSGCGGSYEDTIAGVTVPVPKS
                     MTKSSEAPVEMKFFGFGAGQGSYHGKMESDKIIEFYKKELPARGWQPSASMRGGGAML
                     AYSKEAKTLMVAVGKQNDETSLTVTVGGTGR"
     gene            657..1127
                     /gene="bfr"
                     /locus_tag="VFY96_08485"
     CDS             657..1127
                     /gene="bfr"
                     /locus_tag="VFY96_08485"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEX5606537.1"
                     /translation="MKGNEQVIAVLNQVLRKELTGINQYFIHAKMCENWGYGKLKKVL
                     WDESIGEMKHADKVIERLLFLEATPNMSNYDKIMVGSGVKQQLENDLGLELAALAILH
                     PGIKTCLETSDDASRELLEHILVDEEKHVDWIETQLHQIEEVGYQNYLAQQIET"
     gene            complement(1284..2150)
                     /locus_tag="VFY96_08490"
     CDS             complement(1284..2150)
                     /locus_tag="VFY96_08490"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5606538.1"
                     /translation="MLPIFFGILTSLCFAIASLLAQRGYHTGQAPWGAWITIAANCVF
                     LLAAHFLLRSDTRWLARDDLIFVAVGLFVPGVTRVLSFRGIRTLGSSTTSTIVNTTPM
                     FSTILAILILGERPAPMVLLGVLLTVAGLAAVSWSRVQTSYRKTELIYPFLCALLFSM
                     KDIAVRWGLAGGGGQPILAAGIAALTSSVEIFLIVRYVQGEKFHWPPAAITRWFVASG
                     IFTGGSFLFMYLAYSMERVSIVAPLVNSYTVFVSLLTPLMARDIETVTGRKLAGAALV
                     VAGIFALSLGKD"
     gene            2223..>2465
                     /locus_tag="VFY96_08495"
     CDS             2223..>2465
                     /locus_tag="VFY96_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002618011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX5606539.1"
                     /translation="MPAKDFSTVRRKLLAWYNHNQRNLPWRKTTDPYAIWVSETMLQQ
                     TQVKTVLPYYDRFLNRFPTVEALARAPLDRVLHAWAG"
BASE COUNT          617 a          672 c          679 g          497 t
ORIGIN      
        1 tttgtcggct cgtcgctcgg aggcatgctc gcaggcgggc ttgttcacgt ctcaccggcg
       61 ctgccggagc tcgtcggcgt cgctctgctc attgtttggt attttctcgg cttgccggtt
      121 ccaccggaac ccggctcata ataatcaagc tttttccgtt gatcgtcgcg catgcttggt
      181 gtactcagaa cggcgggaga ttatgctcag gccaatagaa aagttgagtc tcggcgtcat
      241 tatcatcgct gtattctacg cattcggctt gtcaggttgc ggtgggtcgt acgaagatac
      301 cattgccggc gtcacggtgc ctgtgccgaa gtcgatgacc aagagctcgg aggcgccagt
      361 tgaaatgaag tttttcggat tcggcgccgg ccagggttcg tatcatggaa aaatggagag
      421 cgacaagatt atcgagttct ataaaaaaga gctgcccgcc cgcggctggc agcccagcgc
      481 gagcatgcgc ggcggcgggg ctatgttggc ctatagcaaa gaagcaaaaa cgttgatggt
      541 cgccgtcggc aaacaaaacg atgagaccag cttgaccgtg acggtcggag gaacgggtcg
      601 ttagatggcg catttggtgc cgagcgcatc agagcgtcga atcaaagggg gacgtcatga
      661 agggaaatga acaggtcatc gccgtgctca atcaggtttt gcgcaaagag ctgaccggca
      721 tcaatcaata cttcattcac gccaagatgt gcgagaactg gggttacggc aagctcaaga
      781 aagttttatg ggacgaatcc atcggcgaaa tgaagcatgc cgataaagtt atcgagcgac
      841 ttttgtttct cgaagcgacg ccgaatatgt ccaactacga taaaatcatg gtgggcagcg
      901 gcgtaaagca gcagctggaa aatgatctcg gtctggaact cgcggcgctg gccattctcc
      961 acccgggcat caagacctgc ctggaaacgt cagatgacgc atcgcgcgag ctgctcgagc
     1021 acattctcgt ggacgaagaa aagcatgtgg attggatcga gacccagttg caccagatcg
     1081 aggaagtcgg ctatcaaaac tatctggccc agcagatcga gacctgaagc agtctgccgc
     1141 cggaaagatt tctcccggag ttgagcctga gtctgccgaa ggggtcgtaa tgacaagctc
     1201 tgtcatctcg aacgacagtg agagatcttt ccctgttctt caacggttca atctttctat
     1261 cttctagcct gtatctttgg accttaatct ttacccagag acagcgcgaa tatcccggcc
     1321 accaccagcg cggcgccggc gagtttgcgt ccagtaacgg tttcgatatc acgcgccatc
     1381 agcggcgtca ggagcgaaac aaacaccgta tagctattga cgagcggagc cacgatcgat
     1441 acccgctcca tgctgtaagc gagatacatg aacaagaaag aaccgccggt aaaaatgcct
     1501 gacgccacga accagcgtgt tatcgcggcc ggaggccagt gaaacttttc tccctgcaca
     1561 tagcggacaa tgagaaatat ctccacgctt gaagtcagag cggcaatgcc ggcggcgaga
     1621 atgggttgtc ctccgccgcc cgctaatccc cagcgcacgg caatatcctt catcgagaac
     1681 aatagcgcgc aaagaaaagg atagatcaat tcagtctttc tgtaagaagt ctggacccgg
     1741 ctccaggaaa ctgccgccag cccggcgacg gtcagcaaaa ccccgagcag aaccatcggc
     1801 gccgggcgct cgccaagaat caggatcgcg agaatcgtgg aaaacatcgg cgtggtattc
     1861 acgatcgtcg aagtcgtcga gcttcccagt gtgcgaatac cgcgaaagct caagacccgc
     1921 gttactcccg gcacaaacaa accaacggca acgaagatga gatcatcgcg cgccaaccat
     1981 cgagtatcgg agcgcaggag aaaatgcgct gccagcaaga acacgcaatt cgcagcgatg
     2041 gtgatccagg cgccccatgg cgcctgtccg gtatgataac cgcgctgggc cagcagactc
     2101 gcaatggcaa aacagagtga agttaagatg ccaaaaaaaa tcggcagcat cgcgcgaatt
     2161 gtttcccttc agatgaaatt tcgactacag tgcccgtaat cgctaccgcg ctcaaccgcc
     2221 cgatgccggc aaaagatttt tccacggtgc gccgaaaact tctcgcctgg tacaaccaca
     2281 atcagcgtaa tctgccctgg cgaaaaacca ctgatcctta cgcgatttgg gtttcggaga
     2341 ccatgctgca gcaaacccaa gtgaagacgg tactccccta ctacgaccgt ttcttgaacc
     2401 gttttcccac cgtcgaagcg ttagcgcgag cgccgctcga tcgcgtcttg catgcttggg
     2461 ctgga
//
LOCUS       DASULZ010000417         2240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_559272, whole genome shotgun sequence.
ACCESSION   DASULZ010000417 DASULZ010000000
VERSION     DASULZ010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559272"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..1960)
                     /locus_tag="VFY96_08500"
     CDS             complement(8..1960)
                     /locus_tag="VFY96_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5606540.1"
                     /translation="MEPFTFGGDIVWWPSESLVAQSRLKHFMDRHAIATAAELHEKSI
                     ANPEWFWNSVLEALQIEFYEPYSAVVDLSQGIEWPRWCVNGKMNVVHNCLDKWIGTAT
                     ESRVAIRWEGEEGATRVITYGELYREVNRFVNGLRQLGLKKGEVVGIFMPMVPEIAVA
                     TFALAKIGALILPLFSGFGAAAVAARLMDAQATGLITADGFYRRGGAVDLASVASEAL
                     LGASSIKRTIVLPRLGTATVRSGAGRLSWEEVIAGQSEKSATERTEADDSLMIIYTSG
                     TSGKPKGALHTHCGFPIKAAQDMMHGLDVSANDTLYWVTDMGWMMGPWELFGATLHGA
                     TMLFYDGALDHPDPSRLWRLVEEHGVTILGVSPTLVRVLMRHGDESVEQHDLSKLRIL
                     ASTGEPWNPEPWRWLFEKVGKKRLPIINYSGGTEIAGGILMGNVLTPLKPCAFSGPLP
                     GMAADVVDDNGRPVRNQVGELIIRQPWIGMTRGFWHDPQRYLQTYWSRFPKIWLHGDW
                     AAIDEDGLWYVLGRSDDTIKVGGKRVGPAEIESILVEHPSVSEAAAIAVPDPLRGEAV
                     ICFCVLISGEHGDEALAEALKEKVARSLGKPLKPEAIKFVGDLPKTRNAKIMRRVIRA
                     GYLGQEMGDLSALENPLAVDEIRRAS"
     gene            complement(1961..>2240)
                     /locus_tag="VFY96_08505"
     CDS             complement(1961..>2240)
                     /locus_tag="VFY96_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606541.1"
                     /translation="LFKTSALQSHGIAPLIDGIEKRAAELNFDAKRTEKSLKISRAQM
                     LALLRDQLMARLEAKLDDHEIEQWTQRIADKHTDPYTAAEMILAKAGL"
BASE COUNT          471 a          731 c          561 g          477 t
ORIGIN      
        1 tccggcttca agaggcgcgt cgtatttcat cgaccgccaa aggattttcc agcgccgata
       61 aatcgcccat ctcctggccc aaataaccag cgcgaattac ccggcgcata atcttcgcat
      121 tccgcgtttt cggcagatca ccgacgaact ttatcgcctc cggcttgagc ggcttgccaa
      181 gcgagcgggc gaccttttct ttcagcgcct ccgccaaagc ttcatcgccg tgctctcctg
      241 atataagaac acaaaaacag atcaccgctt caccgcggag cggatcggga acagcaatcg
      301 cggccgcctc gctcaccgat ggatgctcga ctaaaatcga ctcgatttcg gcgggtccga
      361 cccgtttgcc acctactttg atcgtatcgt cggatcggcc gaggacatac cacaagccgt
      421 cttcatcgat tgccgcccaa tcgccatgca gccagatttt cgggaaccgg ctccagtaag
      481 tctgtaaata tctttgcggg tcatgccaaa agccgcgcgt cattccgatc cagggctggc
      541 gaatgatcag ttccccgacc tgattccgca ccggccgtcc gttatcgtcg acaacatccg
      601 ccgccatgcc gggcagcgga cctgaaaacg cgcacggctt gagcggcgtc agaacgttcc
      661 ccatcaggat gccgccggcg atttctgtcc cgccagagta gttaataatc ggcaaccgct
      721 tcttgccgac tttttcgaac agccaccgcc atggctcggg gttccagggc tcgcccgtcg
      781 aagccaggat acgaagcttc gagagatcat gctgctcaac ggattcatcg ccatggcgca
      841 tgagcacgcg cacaagcgtc ggcgagacgc cgaggatcgt cacgccgtgc tcctccacga
      901 gccgccacaa acgtgacggg tcaggatggt caagcgcgcc atcgtaaaac agcatggtcg
      961 cgccgtgcag agtcgcgcca aaaagctccc acggccccat catccagccc atgtccgtaa
     1021 cccaataaag ggtgtcgttg gcgctgacat cgagaccatg catcatgtcc tgcgcggctt
     1081 tgatgggaaa accgcaatgg gtatgcagcg cacctttggg ttttccgctc gttcccgatg
     1141 tatagatgat catcagagag tcgtcggcct cggttctttc ggtggcgctc ttctccgact
     1201 gtccggcaat gacttcctcc caggataacc ggcccgcgcc gctcctcacc gtcgcggttc
     1261 ctaagcgcgg aagcacgatc gtccttttga tgctcgaagc tccgagcaga gcttcactcg
     1321 caacggacgc gagatccaca gctccaccgc ggcgatagaa cccatccgcc gtgatcaatc
     1381 ctgtggcctg tgcatccatc agccgcgcgg caaccgcagc agcaccgaac ccggagaata
     1441 gcggcaaaat cagagcgccg atctttgcca atgcaaatgt cgcaacggcg atctccggca
     1501 ccatcggcat gaaaattccc accacttctc cctttttgag gccgagctgt cggagcccgt
     1561 tgacgaagcg attcacttca cggtaaagct cgccatacgt aatcacccgg gtcgcgccct
     1621 cttccccttc ccagcggatt gccacccggc tttccgtcgc cgttccaatc catttatcca
     1681 aacaattgtg aacaacgttc atcttgccgt tgacgcacca gcgcggccat tcgattccct
     1741 gcgacaaatc aacgaccgcg ctatagggtt cataaaattc gatttgcagc gcttccagca
     1801 cgctgttcca aaaccactct gggttggcaa tcgacttctc atgcaattca gccgcagtcg
     1861 caatggcatg tcggtccatg aaatgcttta gccggctctg cgccaccaat gactccgagg
     1921 gccaccaaac gatgtcgccg ccgaacgtga acggttccac ttataatcct gctttcgcca
     1981 ggatcatttc tgccgcagtg tacggatcgg tatgcttatc agcgatcctt tgcgtccact
     2041 gctcgatctc atgatcgtcg agctttgctt cgagcctggc catcagctga tcccgaagca
     2101 gcgccaacat ctgagcgcgg gaaatcttca ggcttttctc tgtccgcttg gcgtcaaagt
     2161 tcaattcggc cgcacgcttc tcgatcccgt caatgagcgg cgcaatgccg tggctctgaa
     2221 gcgccgacgt tttgaatagt
//
LOCUS       DASULZ010000418         3610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_761886, whole genome shotgun sequence.
ACCESSION   DASULZ010000418 DASULZ010000000
VERSION     DASULZ010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761886"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VFY96_08510"
     CDS             complement(<1..203)
                     /locus_tag="VFY96_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606542.1"
                     /translation="MRRQLGFALVFVWSLTTAASTPDRMTPVKITDGVYMLQHSRGSG
                     NSTAVFTPDGVLVFDFAIDNADQT"
     gene            complement(223..1107)
                     /locus_tag="VFY96_08515"
     CDS             complement(223..1107)
                     /locus_tag="VFY96_08515"
                     /inference="COORDINATES: protein motif:HMM:NF026954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX5606543.1"
                     /translation="MELLRSWMFVPGHRQRMIDKALSLDADAIMLDIEDGVAPNEKDS
                     ARKNIADSLGRDKAPGTPARYVRINAIGHARMNADLEAVVRPGLEGLVCPKVDTPDEV
                     RTVEAILNEKEPSNGLPKGSVKLLIAIESPRGLLNAAAIAASSPRISGIMFGAEDFGR
                     EIGLPAVRAGQARDLLYARSSMVIAAASAHIQAVDGVWVDLNDTQGLLGFAKQSRELG
                     FSGMSCIHPAQVQAINNTFSPTAEEIDYCQRVLQAFAEANARGDGSIAFGGQLIDRPI
                     VERARRTIEMAKSLNLIE"
     gene            1360..2565
                     /locus_tag="VFY96_08520"
     CDS             1360..2565
                     /locus_tag="VFY96_08520"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5606544.1"
                     /translation="MKLSTVKLLMPMVAVSIQANSATTIIPPFLDHMRIPVALIGTLI
                     SLGPVLALLSRLPVGMAYSHHRARLVVSASMIAMGLTNYLYSFATGPLTFAVVQGING
                     FAYGAVTTLYMAYYVDSLAPDENRNHAMGYYVGSLAMGYSTGNFFGGLVADHWGYNAT
                     FQLGALLSLIGVGLLWLFNGPTDATVKTGGLKSSGKLSWAESLKALLEPELATVVIVA
                     LFLNVVHQMGSVFISLYGLSVGMTLTQVGIVRAAYAGCNAVTRPISGHVVNRLGHKGL
                     SYFGLPLQSLILMLVPLFTTFGTVLPVYVCSGLLRAIVIVANAVGLVQDVPESRVRRG
                     LASAVYNAAGDLGNILGPSIGGLIAQATGVASVFVIGSLGSTALFFIFVLLLRRFKQP
                     ETETVPSGI"
     gene            complement(2571..>3610)
                     /locus_tag="VFY96_08525"
     CDS             complement(2571..>3610)
                     /locus_tag="VFY96_08525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5606545.1"
                     /translation="VRKLSELGWLAALIPEEYGGTGLGITEASIILEEINRSGGVATA
                     CHAQMYTMGTLLRHGNEEQKKRYLPKIATGELRLQAFGVTEPNAGSESTKIETTATRE
                     GDKYLINGQKIFISRVLQSDLMLLLARTTPYDELEDKTHGLSVFIVDLREAKGKLEIK
                     PLDLMINHHTNALFFDNVEVPLENLIGQEGMGFRYIIDGWNAERILVAAEAIGDGRWF
                     VERAARYASERVVFGRPIGSNQGVQFPIAKAYANIEAADLVRYQAATKFDRKEKCGAE
                     ANLAKYLASEAAWEAANACLTTHGGYGFATEYDVERKFRETRLLSVAPVSNNLVLAYL
                     GQHVLGMPKSY"
BASE COUNT          695 a          987 c         1028 g          900 t
ORIGIN      
        1 gtctgatcgg cgttgtctat ggcaaaatcg aaaaccagca cgccgtcagg ggtaaagacc
       61 gcagttgagt tgcccgatcc tcgagaatgc tgcagcatgt aaacaccgtc ggtaattttt
      121 actggtgtca tgcgatcggg cgtggaagcg gccgtggtta gtgaccagac aaaaaccagc
      181 gcgaacccca gttgtcttct catcggttgt tccttcgttg tgttattcga ttaggttcag
      241 agattttgcc atttcgatgg tgcggcgggc gcgctcgacg atcggccgat cgatcagttg
      301 gccgccgaag gcgattgaac catcgccgcg ggcattggct tctgcaaacg cttgcaacac
      361 cctttggcaa taatcgattt cttcggcggt cgggctaaac gtgttattga tcgcctgaac
      421 ttgggccgga tgaatgcatg acatgccgga aaatcccagc tcgcgcgatt gtttggcgaa
      481 tcccaacaac ccttgagtgt cgtttagatc cacccatacg ccgtccaccg cctggatatg
      541 cgccgacgcc gcggcaatca ccatcgagga gcgagcgtaa agcagatcgc gtgcttgacc
      601 cgcgcgcacc gcgggtaggc caatttcgcg cccgaagtcc tcagcaccga acataatgcc
      661 gctgatacgt ggagatgagg ccgcaatcgc ggcggcgttc agcaaaccgc gcgggctttc
      721 gatggcgatc agaagcttga cgctgccttt cggcaatcca ttcgatggtt ctttttcatt
      781 aaggatcgcc tcgacggtgc gaacttcatc gggcgtgtcg accttcggac agacgaggcc
      841 ttcgaggccc ggacggacca ccgcttcgag gtccgcattc atgcgggcat ggccgatggc
      901 gttgatgcga acgtaacgtg ccggtgttcc cggcgccttg tcacggccga gagagtcggc
      961 gatgtttttg cgcgcgctgt ctttctcgtt cggcgcgacg ccgtcttcga tatcgagcat
     1021 gatcgcgtcg gcgtcgaggc tgagcgcctt gtcgatcatc cgctggcgat gtccgggcac
     1081 gaacatccaa gagcgtagta attccatagt gcactcctcg catctgatta cctgaaggtc
     1141 gggcaaatgt ctaatctgcg ttagacaatg cgaagccgct ctgtgcttct aagcacagtc
     1201 cgcgaaagca tcaaagcctt cagtgcatat cttgtattgc aggaacgtaa tggatgaagg
     1261 aaggatttct cgcgtcgctc gaaatgaccg cactccgtaa gagatttttc ggcctattcg
     1321 gttgaacgcg ggaaaatttt aaagaataac tgaagggata tgaaacttag cacggtcaag
     1381 cttcttatgc cgatggttgc ggtttcgatt caagcgaact cggcaacaac cattatcccg
     1441 ccttttctcg atcatatgcg tattcccgtc gcgttgatcg gcaccttgat ctcgctaggg
     1501 ccagtgctgg cgctgctttc acggctgccc gtaggcatgg cgtacagcca tcatcgtgcc
     1561 cgactcgttg tctctgcgtc gatgatcgcc atgggtctca cgaactatct ttatagcttt
     1621 gcgacaggtc cgctgacgtt cgccgtcgtt caaggcatca atggctttgc ttacggcgcc
     1681 gtcacgacac tctacatggc gtattatgtg gattcactgg cgcccgacga aaatcgcaat
     1741 catgccatgg gctattatgt cggcagtttg gcgatgggtt attcgacggg gaattttttt
     1801 ggtggcctgg tggcggatca ctggggttac aacgcgacgt ttcagctcgg agcgttgctg
     1861 tcgctgatcg gcgtcggatt gctttggctc tttaatgggc cgaccgatgc gacggtcaag
     1921 acgggcggac ttaaatcaag cggtaagctt agttgggccg agtcgctgaa agcgctcctg
     1981 gagcccgagc tcgccaccgt cgtgattgtc gcgttgtttc tcaatgtggt gcatcaaatg
     2041 ggcagtgtgt ttatttcact ttacggattg tccgtcggta tgacgctcac gcaggtgggc
     2101 atcgttcgcg cagcttacgc cggttgcaac gcggtgaccc gccccatcag tggccacgtc
     2161 gtgaatcggc ttggtcacaa aggtttgtcg tattttggcc tgcctctcca gtcattgatt
     2221 ctgatgctgg tgccgctgtt caccacgttc ggcactgtcc tgccggttta cgtctgttcc
     2281 ggtttgttgc gtgcgattgt catcgtcgct aacgcagtgg gcctcgtaca ggacgtgccg
     2341 gagtcgcgtg tgcgccgcgg tttggcgtcg gctgtctaca atgctgcagg cgatctcgga
     2401 aatattctcg gtccttctat tggcggattg attgcccagg ccaccggggt tgcgagcgtg
     2461 ttcgtgatcg gctctttagg ctcgacagcg ttgtttttta tctttgtgct tttgctccgg
     2521 cgcttcaagc aaccggaaac ggaaaccgtt ccgtctggaa tttaggcaga tcagtacgac
     2581 ttcggcattc ccaaaacgtg ttgacctaaa tacgcgagca ctaaattgtt gctcaccggc
     2641 gcgacgctca gcaggcgcgt ctctcgaaat tttctctcga cgtcatattc ggtggcaaag
     2701 ccgtagccgc cgtgagtggt gagacaggca ttggctgctt cccacgccgc ctcggatgcc
     2761 aggtacttgg cgagatttgc ttccgcaccg catttttcct tgcgatcgaa tttcgtcgcc
     2821 gcctgatagc gcaccagatc cgccgcttcg atgttcgcgt atgctttcgc gatgggaaat
     2881 tgcacgcctt ggttcgaccc gatagggcgg ccgaagacga ctcgctcgct cgcatatcgc
     2941 gcggcgcgct cgacaaacca gcggccatcg ccgatcgcct cggcagcaac gagtattcgc
     3001 tcggcgttcc agccatcgat aatatagcga aaacccatac cttcctgacc gattaggttt
     3061 tcaagcggca cttcaacatt atcgaaaaag agcgcgttcg tgtggtggtt gatcatgaga
     3121 tcgagcggct ttatctcgag ttttccttta gcttcgcgga gatcgacaat aaagaccgag
     3181 aggccgtggg ttttgtcttc tagctcgtcg tacggcgtgg tccgggcgag cagcagcatt
     3241 aaatccgact gcaggacacg ggagataaaa atcttctggc cattgatcag gtatttgtcg
     3301 ccttcgcgcg tagccgtcgt ttctatcttg gtgctttccg aaccggcatt gggttccgtt
     3361 acaccgaacg cctgcaggcg tagttcgccg gtggcgattt tcggcagata acgcttcttt
     3421 tgttcctcat tgccatgacg cagcagcgtg cccatggtgt acatctgcgc gtgacaagcg
     3481 gtggcaacac caccagaacg attaatttcc tcgagaatga tgctcgcctc ggtgatgccg
     3541 aggccggtgc cgccgtattc ctcggggatt aacgccgcga gccagccaag ctctgacagc
     3601 ttgcgcacga
//
LOCUS       DASULZ010000419         2828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_559638, whole genome shotgun sequence.
ACCESSION   DASULZ010000419 DASULZ010000000
VERSION     DASULZ010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559638"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VFY96_08530"
     CDS             <1..685
                     /locus_tag="VFY96_08530"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX5606546.1"
                     /translation="AEINELAIQAEIEAAQVGVKDWEVWAAVHYAMTRNGSELPVHCF
                     WVSGKNPKRTLTRPSMRLLERGDVIINEIEASWIGYRAQAVQPVFVEVIHPVQAELIK
                     VQREIFNRVMENLKPGTTVGELAQLSRRTAESAAPKIGPAAGARGDLTMHGRGAGDDG
                     PIITPHARSPKQLGVAMRENMVFICKPSAISAGGEYTCQWGDTVVVTKNGGRRLGKRP
                     HELAVAKQD"
     gene            788..1750
                     /locus_tag="VFY96_08535"
     CDS             788..1750
                     /locus_tag="VFY96_08535"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX5606547.1"
                     /translation="MPLLLSRDELTPLLDLPKAIDLIEEAHRQQAKGQIVPHAPYHLR
                     VGERSGLRVVSGALLEPRRVGVRLGPNSGLGGGNKMYALLFDADSGELLSFMGFPFGT
                     LRTAAVVAIAAKHMAREDSQRLGLFGVGRNAFGILKGLLSVRPIKEVLVSSRDPERRK
                     NFCAEGEKLLGITVRGVEKPEKAVRGMDVILTATNSLTPIFPQEWVDPGTHVSSIGKP
                     TELSRGLHLRADRIVVGSQEQERNYADRAAALPLVELTAEGKLSWSRIPELGELVSGA
                     VTGRANRDEINIFRESQGGYGDMAFATWLYEEAKRRGLGKQMEL"
     gene            1858..>2828
                     /locus_tag="VFY96_08540"
     CDS             1858..>2828
                     /locus_tag="VFY96_08540"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606548.1"
                     /translation="MRMITRKEIVRIFCLIAGVMMMPAAAKAQTLAQLIDGAKKEGQL
                     VLSWGTGTMGGIEGAQTMEKAFNKKYGLNLPFKFSPGPAMPQFASRIIQEEKAGQPAS
                     SDLFIGSENHVARMSLKMLNWPKIFPHISKPMMDWDGRVLIVVSRTPGFTYNTNMIRA
                     NEVPQTVEDTLNAKWKGKIASTPYAASFDRLALIWGEEKTTAFMQKFTKQVAGLIRCG
                     EEERVANGEFAMLVFNCDLADANDMRQKGAPVNGRIFKDAGILSYWYLAVPSNSAHPN
                     GASLLAAFLLTKEGQEILWQTEKTSSHLVEVTYMYKLVKEQERQGVK"
BASE COUNT          660 a          728 c          889 g          551 t
ORIGIN      
        1 ggcggagatc aacgaattgg cgattcaagc ggagatcgaa gccgcgcagg ttggcgtcaa
       61 ggattgggag gtctgggctg cggtgcacta cgcgatgacc cgcaacggct ccgagctacc
      121 ggtgcattgt ttctgggttt cggggaaaaa tcccaaacgc acattgacac ggccgagcat
      181 gcgcttgctc gaacggggcg atgtcatcat taacgaaatc gaagcgtcgt ggatcggcta
      241 ccgcgcccag gcggtgcagc cggtgttcgt cgaagtgatc catccagtgc aggcagagct
      301 gatcaaagtg cagagagaga ttttcaatcg agtcatggaa aatctgaagc cgggaaccac
      361 cgtcggtgag ctggcgcagc tctcgcgccg gacggccgag agcgctgcgc ccaaaatcgg
      421 ccccgccgcc ggcgcgcgcg gcgatctcac catgcacgga cgcggagccg gcgatgatgg
      481 gccgattatc acgccgcacg caaggagtcc aaaacaactc ggcgtcgcga tgcgcgagaa
      541 catggttttc atctgcaagc cgtcggcaat ttcggcgggc ggtgaataca cctgccagtg
      601 gggggacact gttgtggtga ccaagaacgg cggacgcagg ctcggcaagc gcccgcacga
      661 gctggcggtg gcaaaacagg actgaggact gggcaatgag gactgagtgg cgggattcat
      721 taaaaatttc tttaaacctc agccctcgtg actcagtcct gtagtcctaa taagcggagc
      781 gagtgagatg ccgttattgc taagccggga tgaattaacc ccactactcg atctgccgaa
      841 ggcgatcgat ttgattgaag aagcgcaccg gcaacaggca aagggccaga tcgtgccgca
      901 tgcgccgtac catcttcgtg tcggcgaacg cagcgggctg cgggtagttt cgggcgcgct
      961 tctggaaccg cggcgagtag gcgtacgact cggtcccaat agtggtctcg gcggcgggaa
     1021 taaaatgtac gctttgttgt tcgatgccga ttccggtgag ctgttgtcgt tcatgggatt
     1081 tccctttggc acgctcagaa ccgcagcggt cgttgcgatt gcggcgaagc acatggcgcg
     1141 cgaagattca caacgcttgg gtctgttcgg cgtcgggcgt aacgcattcg ggatcctcaa
     1201 aggcttgttg tcggtgcggc caatcaaaga agtcttggtc tcgagtcgtg atcccgagcg
     1261 gcgcaaaaat ttctgcgccg aaggtgaaaa actgctcggc atcactgttc gtggcgtgga
     1321 gaagccggaa aaggccgtgc gcggcatgga cgtgatcctc accgcgacga attcgctgac
     1381 gccgattttt ccccaggagt gggtcgaccc cggaacccat gtgagtagca tcggcaaacc
     1441 gacagagtta agccgcgggt tgcatttacg cgccgatcgg atcgtcgtcg gcagtcagga
     1501 gcaggagcgc aattacgctg accgtgcagc ggcgctgccg ctggtcgaac tcaccgcgga
     1561 aggaaagcta tcctggagcc ggatcccgga actgggcgag cttgtctccg gagcagtgac
     1621 cggcagagca aaccgagacg aaatcaatat ctttcgtgag tcgcagggcg gttatggcga
     1681 tatggcgttt gccacttggc tttatgagga agcaaagcgg cgcgggttgg gaaaacagat
     1741 ggagctttaa tcggaggggt gcttcaggtc gttcaagccg ttcaagatgt tcaagcagtt
     1801 cgaatcgcgg gatgctcaag agcgcagtgt tcgaagttca agattcggat tcgggggatg
     1861 agaatgatta cgcgtaaaga aatcgttcgg atattttgtc tgatcgcggg agtaatgatg
     1921 atgcctgctg ctgcgaaagc tcagacgttg gcgcagctca tcgacggcgc gaaaaaggaa
     1981 gggcagctcg tgttgagctg gggcacagga acgatgggcg ggattgaagg cgcacagacg
     2041 atggagaagg cattcaataa aaagtacggt ttgaatttgc ctttcaaatt cagcccgggc
     2101 ccagcgatgc cgcagtttgc aagtcggatt attcaggagg agaaagccgg tcagccggcg
     2161 agcagcgatc tgtttatcgg ctcggaaaat cacgtcgctc gcatgtcgct caaaatgctc
     2221 aactggccga aaattttccc gcacattagc aagccgatga tggattggga cggtcgcgtg
     2281 ttgatcgtgg tatcgcgcac gccggggttt acctacaaca ccaatatgat ccgcgccaac
     2341 gaagttccac aaaccgtcga agacacgctg aacgccaaat ggaagggaaa aatcgcctcg
     2401 acaccttatg ctgcctcctt cgaccgcctg gcgctaatat ggggcgaaga aaagaccacg
     2461 gccttcatgc aaaaattcac caagcaggtg gccggtctaa tccgttgcgg agaagaggag
     2521 cgcgtcgcca acggcgaatt cgccatgctc gtattcaact gcgatctcgc cgacgccaac
     2581 gacatgcggc agaaaggggc accggtcaac gggagaattt tcaaagatgc cggcattctg
     2641 tcttactggt atctagccgt gccgagcaac tccgcgcatc ccaacggcgc ttcgctgctg
     2701 gcggcattcc tcttgaccaa ggaaggtcag gaaatccttt ggcagacgga aaaaacgagc
     2761 tctcatttgg tcgaagtaac ctacatgtac aaactcgtca aggagcagga gcggcagggc
     2821 gttaagtt
//
LOCUS       DASULZ010000420         5183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_864086, whole genome shotgun sequence.
ACCESSION   DASULZ010000420 DASULZ010000000
VERSION     DASULZ010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5183
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864086"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..989)
                     /locus_tag="VFY96_08545"
     CDS             complement(123..989)
                     /locus_tag="VFY96_08545"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5606549.1"
                     /translation="MTLEPKQQFFEHDGLRLSYWEWGDPRAETYVFVHGVRDQGRSWE
                     HFLEALLRRGVPIKHAVALDLRGHGDSEWPSSSRGYQHEDFLTDLAGLLKHLDKEPST
                     IIGHSLGGSMCLLYAGAFPDKVKRLVLLESLGPFARADDEVPNIIAERLNGRDYVEIP
                     FPHESLESAAKSLQKTFPLIPDQAALHMARHGTTFKGGRYRWKHDPILRYRTTTAMSE
                     GQIEAFIRRLQCPILFVYGTESDFMKSVRGHRAQLFPNAKIVPVDGAGHHIPHEKPEE
                     LAEIVTPFLKQG"
     gene            complement(1104..2090)
                     /locus_tag="VFY96_08550"
     CDS             complement(1104..2090)
                     /locus_tag="VFY96_08550"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606550.1"
                     /translation="MSAKILPLVAGLLLLAAGVGLSEDKPKIPISASSKTLGYSPLWV
                     ASKLGFFDKQGLDVQLVLVNGADKSTMALMGGSVYLSSGGVDTVISAVEQGADLATIG
                     GIINGLTHYIMGAKKFRTFEDLRGANVGSSGLTSGTAFVLRRVLKANGLEYPKDYNLI
                     NVGGSPQAFLSLTAGRIDAAIIAVPLNYEAEQMGYPVIGKVKDFIPNYQLTDLTVKRS
                     WAEKNRPVVVRVMKGMALAMRWLHDNKEPAIDFLAKEMKLKPDQARRGWEYYTENKIW
                     NPNGESNIEGIRTVIQISAERGLFKGGALPPPTKYVDPSYVEEALKEIGRRQ"
     gene            complement(2131..3213)
                     /locus_tag="VFY96_08555"
     CDS             complement(2131..3213)
                     /locus_tag="VFY96_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606551.1"
                     /translation="MKLCGPVSLSSIFGVLLFFAPSYSAQAPFYQGKNITFLINFAAG
                     GPTDIEGRIVARHLAKHIPGNPAVIVQNMSGAGGVTGINFLGEAAKKDALTVGYFTGP
                     YNHHMMKSTTLRTDLMQLPFIASVGSVTVCYIRSDVPPGIKKPTDIAKAERFRAGGLS
                     YDSNKDLRFRLAFDILGLKYDYVTGYNSSNDARLAVQRNEIQYHDENVPGYRGVVEPQ
                     LVKTGIVTPLYYHDVIKPDGSMGKSPDYPELNSFTEIYTQIFGKAPTGIKYEALKAAN
                     MASQNLSRVALLTPNSPKQAVVDLRQAFTSLAKDEEFIAEAKKAMRFQPRFDVGEDGE
                     RLRDKVLRAPDEIVEFVRQYIEQARK"
     gene            complement(3240..4766)
                     /locus_tag="VFY96_08560"
     CDS             complement(3240..4766)
                     /locus_tag="VFY96_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5606552.1"
                     /translation="MRNDLLMGDGLRRSAGKFPLKIAAKDRYRQITYGGLNARVNQLA
                     RGLLSVGLCPGDAVAVSVGNRIEHLEIIFAAAKIGALAIPLDVKWKALELASVLSAFE
                     PRFIVLQEECAGEFAKARRLKDLSFITPVSLSPDRSYSGIVTGQSADEPNVDVNEEDP
                     FAVMLTSGTTGFPKGCLTTHRTFVFHCINNAIEKGLGAHDKALLSSPIYFNAGRSFTL
                     GIIYFGGTMILHERFDAEQVLQAIEGEKITYIGAVPTMCERMLQILETRKYDTSSLRC
                     LAITGGKVHPAVLERLKREMTPNIYRTYASTDSGQMAISKPGDMDAKPSSAGRPVWCV
                     DLRIIDDNHRPVPVGEVGEIICHSPLATHGYYKNPQATHDSFRDGWFYTGDLGYFDEA
                     GFLFVAGRKKDMVKSGGISIYPLEIESVIYSHPDVLEAAVIGVPDPEWGEAVKAVVVL
                     KPGAEMTGDALMGFCKDRLSGYKVPKSVEFRASLPHTEIGKLNKLKLKEMLIAEMAKA
                     "
     gene            complement(4879..>5183)
                     /locus_tag="VFY96_08565"
     CDS             complement(4879..>5183)
                     /locus_tag="VFY96_08565"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5606553.1"
                     /translation="ICLSGELYDAAECHRIGLVNQVVPYPELVPAGEKIAASFLKGAP
                     LALRYIKEAIYKGSEMTLEHGLRYESDLQSMVMQTEDAKEGPRAFVEKRAPDWKGR"
BASE COUNT         1109 a         1489 c         1310 g         1275 t
ORIGIN      
        1 gtatatctct ccggtcaaac gccgtgcctc tgtccaaatc tctaattcct cgaagtacct
       61 gacggtcata attacctcaa cagtttgaac tggttgaacg gtttgaactg gttgaacttt
      121 gttcatccct gcttcaaaaa cggcgtgacg atctcggcta attcctccgg tttttcgtgt
      181 ggaatgtgat ggcccgcgcc atccaccgga acgatcttgg cgttgggaaa aagctgcgcg
      241 cgatggccgc gcacggattt cataaaatcg ctttccgtgc cgtagacgaa aagaatcgga
      301 cattgaaggc gccggataaa agcttcgatc tgtccttccg acatagccgt agtcgtgcga
      361 tagcgtagga tcggatcgtg tttccagcgg tatctgcccc ctttgaaggt cgtcccatga
      421 cgcgccatgt gcagcgctgc ttggtccggt atcaatggaa acgttttttg caacgatttg
      481 gcggccgatt cgagcgattc gtgcgggaat ggaatctcta cgtaatctct cccgttcaac
      541 cgttcggcaa taatgttcgg cacttcgtcg tccgcgcgcg cgaacggacc cagagactcg
      601 agcagcacca accgcttcac tttgtctgga aaggcgccgg cataaagcaa acacatactg
      661 ccgccaagcg aatggccgat aatcgtcgat ggctccttat ccaaatgctt gagcaatcct
      721 gccaaatcgg tcaggaaatc ctcatgctga tagccgcggc tcgagctcgg ccattcgcta
      781 tcgccatgtc cgcgcaggtc gagcgccacg gcatgcttga tcggcacacc gcgccgaagc
      841 agcgcctcga gaaaatgctc ccagctcctg ccctgatcgc gcacgccgtg aacaaacacg
      901 taagtctctg cacgaggatc gccccactcc cagtaactca gccgcaaccc atcatgttca
      961 aaaaattgct gctttggctc taaagtcata gctattgtat cgctgtctcc caccgctggc
     1021 gctaaatcac ttgcccggtt tcttgacgct gcctgttgcg ctttattccg atcgactgct
     1081 gtcgaacaag aataaacatg ttgttattgc cttctgccta tctctttcag tgcttcttcg
     1141 acgtaactag ggtcgacata cttcgtcggc ggcggcaacg caccgccctt gaataatccc
     1201 cgctcggcgg agatttgaat caccgtgcga atcccctcga tattggattc gccattggga
     1261 ttccaaattt tgttctcggt gtagtattcc cacccgcgcc gtgcctgatc gggcttgagc
     1321 ttcatctcct tggcaagaaa atcgatcgcc ggctctttgt tatcgtggag ccagcgcatg
     1381 gcaagtgcca tacctttcat gacgcgaacg acgacagggc gatttttctc cgcccaggaa
     1441 cgcttcaccg tcagatccgt cagctgatag ttgggaatga aatccttgac tttgccgatg
     1501 accggataac ccatttgctc cgcttcgtaa ttgagcggga ctgcaatgat cgccgcatcg
     1561 attctcccgg ccgtgagcga gaggaatgcc tgcggagagc cgcccacgtt tatgagattg
     1621 taatccttgg ggtattccag tccgttcgcc ttcagcacgc gccggagcac aaacgcggtg
     1681 cctgacgtga ggccggacga gcccacattc gcgccgcgca ggtcttcaaa ggtcctgaac
     1741 ttttttgctc ccataatata gtgcgtaagc ccgttgataa tgccgccgat cgtagcgaga
     1801 tccgcgccct gttcgactgc cgagatcacc gtgtcgacgc cgccgctgga gagatagacc
     1861 gatccgccca tcaatgccat cgtcgattta tcggcgccat tgacgagcac gagttggacg
     1921 tcgagtcctt gtttatcgaa aaaacccaac ttgctcgcca cccagagcgg gctatagccg
     1981 agggttttcg acgaggcgct aatgggaatc tttggtttgt cctcgcttag gccgacgccg
     2041 gcggccaaca atagaagccc ggcaaccaac ggtaagattt tcgcgctcac aacagcttcc
     2101 tttctttggg gtgccgacat ttatccgggc ttatttcctc gcttgttcga tatattgccg
     2161 cacgaactcc acgatttcat ccggagctct caatacctta tcgcgcagtc tctcaccgtc
     2221 ctcaccgacg tcgaaacgcg gctggaagcg catcgctttt ttggcctcgg cgataaactc
     2281 ttcgtctttg gccagcgatg taaaggcctg cctcaagtca accactgctt gcttcggcga
     2341 attcggcgtc aagagcgcga cccgactgag attctggctt gccatatttg ccgctttcag
     2401 agcttcatac ttgattcctg tcggcgcctt gccaaaaatc tgcgtataga tctccgtgaa
     2461 agaattgagc tcgggatagt ccgggctctt acccatgctg ccatccggct tgatgacatc
     2521 gtgatagtag agcggtgtga cgatgcccgt cttcaccagc tgaggttcaa caacaccgcg
     2581 gtaacccgga acgttttcat catggtattg aatctcgttg cgctgaaccg cgagccgcgc
     2641 gtcgttgctg ctgttatacc ccgtcacata atcgtatttg agtccgagaa tgtcgaatgc
     2701 cagccgaaac cgcagatcct tgttactatc ataggagagg ccgccggccc tgaaacgctc
     2761 agctttggcg atatccgtgg gttttttgat tccgggtggt acgtcggagc gaatgtaaca
     2821 aaccgttacg cttccgactg aggcgatgaa gggcagctgc atgagatcgg tcctgagtgt
     2881 agtgctcttc atcatgtgat gattataagg tccggtaaaa tagcccacgg tcagcgcgtc
     2941 tttcttcgcc gcctctccga ggaaattgat tcctgtcacg ccgccggcgc cggacatgtt
     3001 ctggacgatc actgccggat ttcccggaat gtgcttggcc agatggcgcg cgacgattcg
     3061 tccttcgata tccgtcggcc caccggcagc gaagttgatt aagaaggtaa tattcttgcc
     3121 ctgataaaag ggcgcttgcg cagaatacga gggagcaaaa aacagcaaca cgccgaaaat
     3181 actcgacaac gatacagggc cgcagagctt catactagat ctcctttccc ttgccactct
     3241 caggcttttg ccatttccgc gatgagcatt tctttcagct tcagtttgtt gagcttgccg
     3301 atttccgtgt gcggcagtga agcacgaaac tccacagatt tgggaacttt gtaccctgat
     3361 aagcggtctt tacaaaatcc catgagagcg tcgccggtca tttcggcacc gggcttgagc
     3421 accacgaccg ctttcacggc ttcgccccat tctggatccg gcactccgat caccgccgct
     3481 tctaggacgt ccgggtggct gtaaatcaca ctctcgatct ccagcggata aatactgatg
     3541 ccgccgcttt tgaccatgtc ttttttgcgt ccggccacga aaagaaaacc tgcctcatcg
     3601 aaatagccca gatctccggt gtaaaaccag ccgtcgcgaa acgagtcatg cgtcgcttgg
     3661 ggatttttgt agtagccgtg cgtcgccaat ggactgtgac agatgatctc gccgacttcg
     3721 ccgaccggta ccggccgatg attatcgtca atgattctta aatcgacgca ccagacgggc
     3781 cttcccgccg agctcggctt ggcatccatg tcgccgggct tggaaatcgc catttgcccg
     3841 gaatcggtcg aagcataagt gcgatagatg tttggtgtca tctctcgctt caagcgctcc
     3901 agcaccgccg gatgaacttt gccaccggtg atcgccagac aacgaagact gctcgtatca
     3961 tattttctcg tttccaagat ctgcagcatg cgctcgcaca tcgtcggcac cgcgccgata
     4021 tacgttattt tttcgccttc gatcgcttgc aggacttgtt ctgcgtcgaa gcgttcgtgc
     4081 aaaatcatcg ttccgccgaa ataaataatg cccagggtaa acgagcgtcc ggcgttgaaa
     4141 tagattggtg atgagagcag tgccttgtcg tgggcgccga gccctttctc gatcgcgtta
     4201 ttgatgcagt gaaagacgaa cgtccgatga gtggttagac aacccttcgg aaatccggtg
     4261 gttcccgatg ttaacatgac cgcaaacgga tcctcttcgt tcacgtcaac atttggctca
     4321 tccgcgcttt ggccagtaac gatgccgcta tagcttctat ccggggagag tgaaaccggc
     4381 gtgataaagc tcagatcctt aagccttctg gccttggcga actccccggc gcactcttcc
     4441 tgcaagacga tgaagcgggg ttcgaacgca gacagcaccg acgcaagttc caatgctttc
     4501 catttgacgt cgagcggaat cgccagcgcg ccgatttttg cagccgcgaa gataatctcc
     4561 agatgctcga tccgattgcc cactgagacc gcaacagcat cccctggaca cagtccgacg
     4621 gatagtagcc cacgcgccag ctgattgacc cgcgcgttca gccctccata ggtaatttgt
     4681 cgatatcggt ctttcgcggc gattttgagg ggaaattttc cggcgcttcg ccgcagccca
     4741 tcgcccatca ataaatcatt tctcattttc gattttagat tttggatttt cgattggaga
     4801 cccacctcaa tctattttgt aattttcgat ttaccgtgct cggacaatat aaaatcgcaa
     4861 atcggcaatc gacaatcgtt aacgtccttt ccagtcgggc gcccgctttt cgacgaaggc
     4921 gcgcggccct tccttggcat cttcggtctg catcaccatg ctctggagat cggattcgta
     4981 gcgcaacccg tgctccaagg tcatttcgct acctttgtag atcgcttcct tgatgtagcg
     5041 gagcgccagc ggcgcgccct tcaaaaaaga tgcagcgatt ttctcgcctg cgggaacgag
     5101 ttcgggatac ggcaccactt gattgaccag gccgatacga tggcattcgg ctgcatcgta
     5161 aagttcaccc gaaaggcaga ttt
//
LOCUS       DASULZ010000421         3560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_355717, whole genome shotgun sequence.
ACCESSION   DASULZ010000421 DASULZ010000000
VERSION     DASULZ010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355717"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VFY96_08570"
     CDS             complement(<1..184)
                     /locus_tag="VFY96_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014029113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5606554.1"
                     /translation="MPIKISELDHIVLNVSDIHRSLDFYTRVLGLSGERVEEFEAGTV
                     GFPSVRINQRTIIDLFP"
     gene            complement(220..1299)
                     /gene="hisC"
                     /locus_tag="VFY96_08575"
     CDS             complement(220..1299)
                     /gene="hisC"
                     /locus_tag="VFY96_08575"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX5606555.1"
                     /translation="MLSIEFAPVPQYFRHNIFAMTGYVPGEQPGGDGVIKLNTNENPY
                     GPSPKVYATLKRAVNSSLRLYPEPQSDTLRAAAAQVYGVNLGNIIVGNGSDEILSMLL
                     RCFVRPGERVAYPVPTYSLYDTLVEIEDGSRAAVNYPADFSLPAELAEQNAALTFVCN
                     PNAPSGTLVGLPAIEKLARSVSGILAVDEAYVDFAATEGASAIPLIYQVPNLVVLRTF
                     SKSFSLAGMRIGMAFAAEEIIAGMMKVKDSYNVNRLSLLAATAALEDLPWMTRNAQRI
                     QRSRKNLTAGLERLGYQVYPSQANFVLARKDGENLRRVYQELKGRKIFVRYFDLPGLQ
                     ECLRITVGTPSEIKILLKEMAILSQ"
     gene            1538..2851
                     /locus_tag="VFY96_08580"
     CDS             1538..2851
                     /locus_tag="VFY96_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606556.1"
                     /translation="MIDAIKSPNPLVLLLNEIDILLAQMRLMELCAHQAQAKVNYERQ
                     CAQQKYEADLATIRHAVAESQRVQTDNAAEAIETKFLENLRTIEDELNKTRCKYQRQI
                     ESLQHELAVRERASTERQEAVSGVELALHGKIQSLQQELARLRSENADLREKTTEAEE
                     LCGEAIGNHLLKADLQKQNGDLRTALSAAQALQRNTEAKLGELRRQLAEAQRIVGSRG
                     AEIDDLKQQLVALNQQQGERASVDTSLQKQPCALTQHQDLSRREMEQSLREEVDRLLH
                     EAEEKSQILQNRNDELVTVKAAMDALQERLSETETTTAGKENAALADVERMRSEFQAQ
                     LALLQAELSQRDWALQERDAMNRSLEQNYRDEIDSLRQRLEEEKSRKVSAIEDFTMGK
                     IQISAVPSVDLTSQVRDHRVDSSVQGRRWHTDFVWKRRWKASETK"
     gene            2927..>3560
                     /locus_tag="VFY96_08585"
     CDS             2927..>3560
                     /locus_tag="VFY96_08585"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX5606557.1"
                     /translation="MLETPRTTTGIARLDHLLEGGFPKGRSFLVTGEPGTGKSIFALQ
                     FIVEGLKRGERCIFVTADEEPGDVLEQAASIGWDLERHVENKELAILNAGAYLSALPG
                     ANRERIVDVQKTVNDLAGFVNQLGASRLALDPAGPFVLLRDSGIRIQDQARLLIKLLR
                     STTRTTNLLTSYAVPRTGERTLHGIEEYLVAGAIVLEMVWKDGALARSLIV"
BASE COUNT          898 a          960 c          948 g          754 t
ORIGIN      
        1 ccggaaaaag atcgatgatg gttcgttgat tgattcgaac cgacggaaac ccgaccgttc
       61 ccgcttcgaa ttcttcgaca cgttcaccgc tcaaccccaa aaccctcgtg taaaaatcga
      121 gggagcgatg aatgtcgctg acgttgagaa caatatggtc gagctcacta attttgatcg
      181 gcatggctcg cctcctgcgc catcagcgca ctacctggcc tattgactta ggatagccat
      241 ttctttcagt aagattttta tctccgacgg tgtgcccacc gtgatccgca ggcactcttg
      301 cagacccgga agatcgaaat agcgcacgaa aatctttcgg cccttgagtt cttggtaaac
      361 ccttctcagg ttttcgccat cttttcttgc caggacaaag ttcgcctgag atggatagac
      421 ttgatacccc aggcgctcga gaccggcagt gaggtttttg cggctgcgtt gaatgcgctg
      481 cgcattgcgc gtcatccatg ggagatcctc gagggcagcg gttgcggcga ggagactcag
      541 ccggttcaca ttgtacgagt ctttcacttt catcatgccg gcgataatct cctccgctgc
      601 aaaagccatc ccgatgcgca tcccggccag tgagaaagat ttggagaaag tccgcaatac
      661 caccagattc ggaacttgat agatcagggg aattgcggac gcgccctcag tggcggcaaa
      721 atcgacgtat gcttcgtcca cagctaagat ccctgacacg gatcgcgcga gcttttcaat
      781 ggccggcaag cctaccaggg taccggacgg cgcgttcgga ttgcatacaa aagtgagcgc
      841 cgcattctgc tccgccagtt cagccggaag agaaaaatcg gcgggataat tcactgccgc
      901 cctgctgccg tcctcgattt ctaccaaggt atcgtacaaa gaatacgtcg gcacgggata
      961 ggccacgcgc tctcctggtc gaacaaaaca acgtaagagc atcgacaaaa tctcgtccga
     1021 gccattgccg acaataatat tcccgaggtt cacgccgtaa acttgtgcgg ctgccgcacg
     1081 taacgtatcg ctctgtggct ctggatacag acgcaatgat gagttcaccg cccttttcag
     1141 cgttgcataa actttcggtg aggggccata tggattttcg ttggtattga gctttatgac
     1201 accatcgcct ccaggttgct cgcccggtac atagccggtc atcgcaaaaa tgttatgacg
     1261 aaaatattgg ggcacgggtg cgaattctat cgacagcaaa ggtccatttc aaattcccta
     1321 aaaacacgac tccgatgact aactgctgac aatattgtcc aagcttagag aagctggtca
     1381 caattcgtgc tcaacaagcc ggacccgttc cacaggcgtt ttcgtttcga ctaactcttt
     1441 gctattgaga catgaacttt cgccgtgtca tctttctggc ttgcgacagc ggtgttcaac
     1501 ccggcaattt gccaatcggc ctacatggat ggtctaaatg atagatgcaa tcaaatcacc
     1561 taatccttta gttctcttgc tgaacgagat cgatatttta ctcgcccaga tgcgcttgat
     1621 ggagctatgc gctcatcagg ctcaggccaa agtaaactat gagagacagt gtgctcaaca
     1681 gaaatatgag gctgacttgg caaccattcg tcacgcagtc gcggaaagtc aacgcgtgca
     1741 gaccgataat gcagccgagg cgattgaaac gaagtttctg gagaatctgc gcaccatcga
     1801 agacgagctg aacaaaaccc ggtgtaaata tcagaggcaa attgaaagtc tgcagcacga
     1861 actcgcggtt agagaacgcg catcgactga gcgccaagag gccgtgtcgg gggtcgagct
     1921 cgcgctgcac ggcaaaatcc aatccttaca acaagagctc gcgcggctcc gttcggagaa
     1981 cgccgatctc agagagaaaa ccaccgaggc tgaggagctg tgcggtgaag ccattggcaa
     2041 tcacctgctc aaggctgact tgcaaaaaca aaatggggat ttgcggacgg ccttgtctgc
     2101 ggcgcaagcg ctgcaacgga acacggaagc aaagcttggc gagctacggc ggcagctcgc
     2161 cgaagcgcag cgaatcgtcg gaagccgcgg agcggaaatc gacgacttga aacaacaact
     2221 cgtcgctttg aaccagcagc agggtgagcg tgcttccgtc gatacctcgc tccaaaaaca
     2281 accctgcgcc ctcactcaac atcaggacct ctcccggcgc gaaatggaac aaagcctccg
     2341 cgaggaagtc gatcgcttgc tccatgaagc agaagagaaa agccaaattt tgcaaaatcg
     2401 caatgacgag ctcgtcaccg tgaaagctgc catggacgct ttgcaggaac ggctgagcga
     2461 aaccgaaact acgacggcgg gaaaggagaa cgccgcactc gcggacgtgg agcggatgcg
     2521 ctcggagttt caagcccagc ttgccttgct tcaggcggag ctcagtcaga gggattgggc
     2581 cttgcaagag cgcgacgcga tgaatcgatc gctcgagcaa aactaccgtg atgagatcga
     2641 ttcattgcgc cagcggctgg aggaggagaa gagccgcaaa gtcagcgcaa ttgaagactt
     2701 cacaatggga aaaatccaga tctccgcagt acccagcgta gatctgacat cgcaggtccg
     2761 tgatcatcgt gtggactctt ccgtgcaggg gcgtcggtgg cacactgatt tcgtctggaa
     2821 aagacgttgg aaggcatccg aaacaaaata gtcaattcga atgggccaac agaattgtcg
     2881 gataaattag acggacccga atcccctttg gactcgagga gaaacgatgc tagagacgcc
     2941 acgtacgaca acaggaatcg cgaggcttga ccacctcctg gaaggagggt ttcccaaagg
     3001 acgttctttt ctcgttaccg gcgagcccgg caccggaaaa tccatctttg cgcttcaatt
     3061 tattgtggag ggtctcaaac gcggtgaaag atgcatcttt gtaaccgcgg acgaagagcc
     3121 cggcgatgtt cttgaacagg ccgcatccat cggatgggac ctcgagcgcc atgtcgaaaa
     3181 caaagagctc gcgattctaa atgccggcgc ttacctcagc gcgttacccg gtgccaatcg
     3241 ggagcgtatt gttgatgtcc agaaaacagt taacgacctc gccggttttg tcaaccagct
     3301 cggagccagc agacttgcgc tcgatccagc cggacctttc gtattgctgc gcgacagcgg
     3361 cattcgcatt caagatcagg cacggttgct cattaagctt ttgcgctcga ccacacgtac
     3421 caccaatctt ctcacttcct acgccgttcc ccgaaccggt gagcgcactc tgcacggaat
     3481 tgaggaatac ctggtcgccg gtgcgattgt tttggaaatg gtttggaaag acggcgccct
     3541 cgcgcgaagt ttgatcgtcg
//
LOCUS       DASULZ010000422         2738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_458110, whole genome shotgun sequence.
ACCESSION   DASULZ010000422 DASULZ010000000
VERSION     DASULZ010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458110"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1561
                     /locus_tag="VFY96_08590"
     CDS             167..1561
                     /locus_tag="VFY96_08590"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX5606558.1"
                     /translation="MLKTSMQAMAEFAFALRYDDIPAEVITAARRHLADSLACAFGAR
                     ETATVQALQKHAIAQGGRADATVIGTAKKVPASMAALVNGTMVRQLDANDIFVLHRGG
                     TSGHFSDATAALLALAEQYRRSGEELLTCLVASYELQGLLADSINFWDRGLHPVTNVA
                     WVTPIIAARLIGAAPVAAVHACGLAVATGTLMNTWIRPAASIPSIKSVAVGLALKRAI
                     EAAELSAVGITATADALETVLARLAPGPDTLFDPVPIENLGQHWSIKRNMLKRYPAQV
                     NTQAAIEAVMRLYQSGIRVEHVRKLVLHGHRNVVGGVQGSTQAFAPATRESADHSTPY
                     VMAMALLRGKLTLDEYAGAPWESAEVKAVMAKIQLVIDDEMDEAFTTRGMLGVRLITE
                     LNSGRTEEFVVDQPKGHPEASLSDLELLEKMSWLMKPEASSVTPQQLLELCNLLSTSE
                     DIQRLIASGRIEHL"
     gene            complement(1620..>2738)
                     /locus_tag="VFY96_08595"
     CDS             complement(1620..>2738)
                     /locus_tag="VFY96_08595"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606559.1"
                     /translation="GPLGYKTFGLRQLTRPQRIRAAKEVTMANVNRTRSCISHQTFLV
                     FLLLHFLASAAAAQELSKKLLVGYAGLISTHASVWLGEDQGFYRKHGVDVTSVFISSG
                     SVTSQALVSGEAKLASTSVGPTAGAVSGGADLAIVAATIHILPYQLWVTPQIRQPADL
                     KGKRVAVSTFGSGSHLAVEVALQHLGLDPARDKIAIIQVGTQPERVASLVTGKIDGTA
                     LEPGFGQAARDKGLTVMADLTKLEAPYLNTVIVASRRYIKENPQQMEAFLKGTIDGLY
                     FLPNPANEKVVKSVVAQRMKLSTPQSIQIMYDATVDIHAKTKTPYATLAGVQNMIDAL
                     MRINPRLAKVKAAEIIDNSFIERLDKSGYIAEAMKRGR"
BASE COUNT          583 a          763 c          803 g          589 t
ORIGIN      
        1 ggggcccgca cagaacctga aagacaagca gcctctacgc gatgggatta tttcagtcaa
       61 gggcactggt tccattgtag aggcgagaac tcatggagtc tgacaacggc cgcacatcat
      121 tggagaggca catcatgaat gacgaagtgc ggcaagcgaa agagcaatgt tgaaaacttc
      181 aatgcaagca atggcagagt ttgccttcgc tctgcgctac gatgacattc cggcggaagt
      241 cattaccgcc gcgcgacggc acttggccga tagcctagca tgcgcattcg gcgcgcgcga
      301 aacggcaacg gttcaagcgc ttcagaaaca tgccattgcg cagggaggac gcgccgacgc
      361 gacggtgatc ggcactgcga aaaaagttcc cgccagcatg gccgctctgg tgaacggcac
      421 catggtgcgc caactcgacg ccaacgacat ctttgtcttg catcgaggtg gcacctcggg
      481 tcacttcagt gacgcgacgg cggcattgtt ggctctcgcc gagcaatatc ggcgcagcgg
      541 cgaggagttg ctgacctgtc tcgtcgcatc ctacgagctg caaggattgc tggctgactc
      601 gatcaatttc tgggatcgcg gtctccatcc ggtgaccaat gtcgcctggg ttacgccgat
      661 tatcgccgcg cgtctcatcg gcgctgctcc cgttgcagcc gtccacgctt gcggtctcgc
      721 cgttgcaacc ggcacgttga tgaatacctg gatcagaccg gcggcgagca ttccgtccat
      781 caaaagcgtc gccgtggggc ttgcgttgaa gcgcgcgatc gaggcggccg agctgtccgc
      841 tgtcggcatc accgcgaccg ccgatgcgtt ggaaacagtc ctggcgcgtt tagctcccgg
      901 gccggacacg ttgttcgatc cggtgcccat cgagaacctc ggccaacact ggagcatcaa
      961 acgaaacatg ctgaagcgct atccggcgca ggtcaacacg caggccgcga ttgaggccgt
     1021 catgcggctt tatcaaagcg gcatccgcgt cgagcatgtg cgcaaactgg ttcttcatgg
     1081 tcatcgcaat gtcgtaggcg gtgtccaagg ttcgacgcag gcctttgcgc cggcaactcg
     1141 ggaatcggca gatcacagta cgccttatgt catggcaatg gcgctgttac gcggcaaatt
     1201 aaccctcgat gaatatgcag gagccccatg ggagagcgca gaagtcaaag ccgtgatggc
     1261 caaaatccag ctcgtgatcg atgacgagat ggacgaggct ttcacgactc gaggaatgct
     1321 cggcgtgcgg cttattacgg aattaaatag tgggcggacc gaagagttcg tcgtagatca
     1381 accgaaaggt catcccgagg cgtccttaag cgatctggag ttgctcgaaa agatgagctg
     1441 gcttatgaaa cctgaagcgt cctcagtaac gccgcagcaa ttactcgagc tttgtaatct
     1501 cctttcgacc tcggaagaca tccaacgact tatcgcaagc ggccgaatcg agcatctctg
     1561 atcgtgcctc atccaattcc tggtgagttg aacgatttcg tgatgatttt gtgcctccgt
     1621 catcggccgc gtttcatggc ttcggcgata taaccggatt tgtccagccg ttcaatgaat
     1681 gagttatcaa ttatctccgc cgctttgact ttggcgaggc gcgggtttat gcgcatcagc
     1741 gcatcgatca tgttttgcac accggccaga gtcgcgtagg gagtcttggt tttggcgtgg
     1801 atatccaccg tggcgtcata catgatctgt atgctttgag gcgttgagag tttcattcgc
     1861 tgcgccacaa cgctcttcac cactttctca ttggccgggt tcggcaaaaa gtacaagcca
     1921 tcgatggtgc ctttcagaaa tgcttccatc tgctgcggat tctctttgat atagcgccgt
     1981 gaggcgacga tgactgtatt caagtagggc gcctcgagct tggtcagatc ggccattacc
     2041 gtgagtccct tatctcgcgc ggcttggccg aagccgggct ccagtgcggt cccgtcaatt
     2101 tttcctgtga ccagggacgc cacgcgctcc ggctgagttc ccacttgaat gatcgcgatt
     2161 ttgtcgcgcg ccggatcgag gccgagatgt tgtagggcta cttcgacagc cagatggctg
     2221 ccggaaccga aggtgctgac ggccactcgc ttgcctttca gatcggcggg ctggcggatc
     2281 tgaggagtga cccagagctg gtagggaaga atgtgtattg tcgccgccac gatggccaga
     2341 tctgcgccgc cgctgacggc acccgccgtg ggtccgacgc tagtggacgc caacttggct
     2401 tccccggaaa ccaaagcctg agaagtcacc gatccgctgc tgatgaatac tgaggtgaca
     2461 tcgaccccgt gctttcgata aaagccctga tcttcgccga gccagacgct agcgtgagtc
     2521 gagattaaac cggcgtagcc gaccaagagc ttcttcgaaa gttcctgcgc ggcggcggcc
     2581 gacgcgagaa aatggagcaa aagaaaaacc aggaacgttt gatgcgaaat gcagctgcgc
     2641 gtgcggttca cattcgccat ggtgacctcc ttcgcggcgc gaatgcgctg tggacgagtt
     2701 aattgtctta gaccgaatgt cttataaccc agtggacc
//
LOCUS       DASULZ010000423         2052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1065819, whole genome shotgun sequence.
ACCESSION   DASULZ010000423 DASULZ010000000
VERSION     DASULZ010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065819"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VFY96_08600"
     CDS             complement(<1..594)
                     /locus_tag="VFY96_08600"
                     /inference="COORDINATES: protein motif:HMM:NF036509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606560.1"
                     /translation="MFSRKPRERLTPAEILRRAGFARDELQLVIEALRGLCRDGQLVR
                     LKKNHYALPDREHLVKGRVHAHPNGYGFLIPENRDLQDLYLNRREMRRVMHGDQVMVR
                     IDRKARGGTESHIVRVLERGQKRLLGTYDELNGKPIVVPMDARISPIPLAKVAAKTEK
                     GKVIAVEVSRYGTALSSPEAQIVNIMGDPNDPEVQAQS"
     gene            complement(717..971)
                     /locus_tag="VFY96_08605"
     CDS             complement(717..971)
                     /locus_tag="VFY96_08605"
                     /inference="COORDINATES: protein motif:HMM:NF027581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4911 domain-containing protein"
                     /protein_id="HEX5606561.1"
                     /translation="MEVPSEQIAYVKFIFESYEDIGIIRTVDRKKAIIVLLAMDDFLA
                     SARAILASLENEIPLRQIARPATISDDWLMAELAVQSTTR"
     gene            complement(1000..>2052)
                     /locus_tag="VFY96_08610"
     CDS             complement(1000..>2052)
                     /locus_tag="VFY96_08610"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEX5606562.1"
                     /translation="QAVAENIEQRRATFTLSSTRINLAEVPLLTASPPVQLDQVDAKG
                     EFQLQNDGSLVTGSIRAARADLYPFRVQDLRSDIVLTPAAFSFKNLSAQMANGTVYSE
                     GYWAPPGGRLQEFDFTSKVAALDLRELATQFFPAVSGRLAGLLSGQARFTAALPEGAN
                     IKDRIETSGEASVQQGTIRDFNLIAHLLLRGSDANISRSRLPPSLAALIDRRDTPFDS
                     LKTNFTVEQKRIRTDNLVITTPEYTLTGAGWIGFDRSTNWNGLLIFSPRVTEEFQRDY
                     RIIRRLLDRRGRLAVSFRVDGKIPNVKIRLDNRALAQAIGTGPAQDKESEPKPGQEPK
                     EVKRWIPDAIERFLNR"
BASE COUNT          440 a          559 c          580 g          473 t
ORIGIN      
        1 cgattgcgcc tgcacttccg gatcattggg gtcgcccatg atattgacga tctgagcttc
       61 gggtgaagaa agcgcagtgc cataacggga gacctccacg gcgatgactt tgcctttctc
      121 ggtcttggcc gccactttgg cgagcggtat cgggctgatg cgggcgtcca tcggaaccac
      181 gatgggcttg ccgttcagtt catcgtaggt gccgagcagg cgtttctggc cacgctctaa
      241 tacccgcaca atatggcttt ccgtgccgcc gcgcgctttg cgatcaatgc gcaccatgac
      301 ttgatcgcca tgcatcaccc ggcgcatctc gcggcggttc aaataaagat cttgcaggtc
      361 tctgttttcc ggaatcaaaa aaccgtaacc attcggatgg gcatgaactc tgcccttaac
      421 cagatgctct cggtcgggaa gggcgtagtg gtttttcttc aaccggacca actggccgtc
      481 gcggcataat ccgcggagcg cctcgatgac aagttgcaat tcatcccgag caaaaccggc
      541 gcgacggaga atttccgccg gcgtgagccg ctctcgcggc ttgcgtgaga acaaggcgag
      601 tatcttgtca ccgaccttgg attccgaagc agacttggga tgtgccggac gtcgcattta
      661 aaagggagtt ggaaattacg tgtcgcgagt tcgcctacac gagaagtcta ttgagtctaa
      721 cgggtcgtgg attgtaccgc cagctctgcc atcagccagt catcgctgat ggtcgccggt
      781 cgggcaattt gccggagcgg tatttcgttt tcaagagacg caagaatggc gcgcgcgctc
      841 gcgagaaaat cgtccatcgc gagaagcacg ataatcgcct ttttgcgatc gaccgttcgg
      901 ataatgccaa tgtcctcgta ggattcaaaa ataaatttca cataagcgat ctgttcgctc
      961 ggaacttcca agtagatctc gtggagcttc atttcggaat taacgattaa gaaaccgctc
     1021 gattgcatcg ggaatccaac gcttgacttc tttgggttcc tgccctggtt taggctccga
     1081 ctctttgtct tgagccggtc ccgtgccgat cgcttgcgcc aatgcgcggt tgtccagtcg
     1141 gatttttacg ttgggaatct ttccgtcaac acgaaatgac acggcaagtc tgccccgtcg
     1201 atcgagcaga cgacggataa ttcgataatc gcgctgaaat tcttctgtca cacgcggcga
     1261 aaagatcagc agcccattcc agttagtcga acggtcgaag ccgatccacc ctgcgcccgt
     1321 aagcgtgtat tcgggagttg tgataaccag attgtcggtg cgaatgcgtt tctgttcaac
     1381 ggtaaaattc gttttgagtg agtcaaaagg cgtatcccgg cggtcgataa gggcggccaa
     1441 acttggcggt aatcgcgacc gggaaatatt ggcgtcgctg cctcggagca gcaagtgcgc
     1501 gatcaaattg aaatccctga tcgtaccctg ctgcacggat gcttctccgg aagtctcgat
     1561 tctgtccttg atgtttgcgc cctccggcag cgcggccgta aatcgagcct gaccgctaag
     1621 caaacccgct agtctcccgc ttaccgccgg gaagaactgc gttgccaatt cgcggagatc
     1681 tagcgcggcg actttggatg tgaaatcgaa ctcctgcaag cgcccgccag ggggagccca
     1741 gtaaccctcg gaataaacgg tgccatttgc catctgcgct gagaggtttt tgaagctaaa
     1801 cgcagccggc gtaaggacga tgtccgagcg caggtcctgc acgcgaaatg ggtaaagatc
     1861 cgctcgcgcc gctctgatgg agccggtcac cagcgatccg tcgttctgaa gctgaaattc
     1921 gcctttggca tcgacttgat cgagttgaac gggcggactc gccgtgagaa gagggacttc
     1981 ggcgagattg atccgcgtcg agctcaaggt aaaagtcgcc cgacgctgct cgatattttc
     2041 agcgacggct tg
//
LOCUS       DASULZ010000424         2663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_458222, whole genome shotgun sequence.
ACCESSION   DASULZ010000424 DASULZ010000000
VERSION     DASULZ010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458222"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VFY96_08615"
     CDS             <1..690
                     /locus_tag="VFY96_08615"
                     /inference="COORDINATES: protein motif:HMM:NF021645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HEX5606563.1"
                     /translation="IAPVVIDVLNMRAQMSRQLQAFGAYSSRLTAYSSRGLLPIPETG
                     AGERLLRMVDPWAYRDRLTLPKLIVNGTNDFYWTTDALNLYWEGIPSNKWVLYIPNAG
                     HNLRRQDRNPREQLDDLVTNLAAFSRYQSTSTPLPDLRWRHENVDGKFRLTVDASPSP
                     IAARLWVAHSETADFRTAQWHAREMTNSDGKLMGEVAAPQIGRQAFFGELGYEIDGLR
                     YHLSTQMRLTP"
     gene            750..1838
                     /locus_tag="VFY96_08620"
     CDS             750..1838
                     /locus_tag="VFY96_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5606564.1"
                     /translation="MKLSTTPDLPGLEDRFFLWLAIAVSLAFAWILWPFFSAVLWGTI
                     LAILFIPLYRRLLKKMARRRSLAAVVTVIIVFLLVIIPLILISALLVQEALSVYERFQ
                     SGEFNPGQYVLQVLDSLPQWVTDVLERFGLTNFASMQEKLSDNLIKGSQFLATKAFNI
                     GQNTFDFVVNVCIVLYLLFFLLRDGEDLTRRIRTAVPLHPSQKHQLFNRFTTVIRATV
                     KGNVVVAIVQGVLGGLAFWFLGVHAPVLWGVVMAFLSLLPAVGSALVWVPVAIYFLIT
                     GALWQGISLIAFGVFVIGLVDNVLRQILVGKDTKMPDYVVLIATLGGMAIFGIHGFVI
                     GPVIAAMFMAVWDILAMTNQARQQNEPA"
     gene            2041..>2663
                     /locus_tag="VFY96_08625"
     CDS             2041..>2663
                     /locus_tag="VFY96_08625"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5606565.1"
                     /translation="MLAIGLALIASFCFAAAMILINRGVLVLDYFRGLLTNLGINALF
                     LWVYLFFVGNRQGLSDRANLIFVLVGVFVPGVARFFIFKGMERLGASITACLTNSTPL
                     FATLFAVFFLRERPTVTNLLGTFAIVCGIVALSWKGSTKSWRTRDLLFPLAAAFLFAG
                     RDNLVRFGLLQIGSPVLGAAIAATTSFLTMSALYIAFEKKLPLASSAV"
BASE COUNT          507 a          751 c          705 g          700 t
ORIGIN      
        1 attgccccgg tggtcatcga tgtgctcaat atgcgcgcgc aaatgtcgcg tcagcttcaa
       61 gctttcggcg cctacagctc tcgactcacg gcatatagta gtcgcggatt gttgccgatc
      121 cccgaaaccg gcgccggcga gcgtcttttg cgcatggtgg acccgtgggc ttatcgcgac
      181 aggttgaccc tgccgaagtt gatcgtcaac ggcaccaatg atttttactg gactaccgat
      241 gcgctgaacc tctattggga gggcattccc agtaacaagt gggtgctcta cattcccaac
      301 gctggccaca atctgaggcg tcaagatcgt aatccgcgcg agcaattgga cgatctcgtc
      361 accaaccttg ctgcgttctc gcgctaccag agcacaagca cgccgctgcc agacctacgc
      421 tggagacatg agaatgtcga cgggaagttt cgcttgaccg ttgatgcgtc gccatcaccc
      481 attgcagccc ggctctgggt cgcacattcc gaaacggcgg atttccgcac cgcccaatgg
      541 catgcgcggg aaatgacaaa ttcggacgga aagctaatgg gcgaggtggc agcgccccaa
      601 atcgggcggc aggccttttt cggagagctc ggctatgaga tcgatggtct gcgctatcat
      661 ctctcgacgc aaatgcgcct gacgccgtag tttcgattgt attcccagac ggctcgtgac
      721 tgacaccgga aaatcgttat tcggatagca tgaagctttc gaccacacct gacttgcccg
      781 gccttgaaga cagattcttc ctgtggcttg ccattgccgt atcgttggca ttcgcatgga
      841 ttctctggcc gtttttcagc gcggttctct ggggaacaat cctggcaatt ctgtttattc
      901 cgctctatcg gcgcttgctg aagaagatgg cacgccgacg ctcgctcgct gccgtcgtga
      961 ccgtcatcat cgttttcttg ctggtcatca ttcccctgat tctgatcagc gctctgttgg
     1021 tccaggaagc gctctcagtc tatgagcgat ttcaatccgg cgagttcaat ccggggcagt
     1081 atgttcttca agtactcgac agcttgccac agtgggtcac cgatgtactg gaacgctttg
     1141 ggctgacgaa tttcgcctcg atgcaggaaa aactgtcaga caatttgatc aaaggcagcc
     1201 aattcctggc gaccaaggct ttcaacatcg gccagaacac tttcgacttt gttgtgaatg
     1261 tgtgtatcgt cctttacttg ctgttttttt tactacgcga cggcgaggat ctcacgagac
     1321 gtatcagaac ggcggttcct ttgcacccga gccaaaagca tcaattgttc aacagattca
     1381 cgacggtcat tcgcgcgacc gtcaaaggca atgtggtcgt cgcgatcgtc caaggcgtgc
     1441 tcggcggctt ggcattctgg ttcctgggcg tgcacgcacc ggtcctctgg ggtgtcgtca
     1501 tggcttttct gtccctgctg ccggccgtcg gcagcgccct cgtatgggtg cccgtcgcga
     1561 tttatttttt aatcacgggg gcgctatggc aaggcatttc tttgatcgca ttcggggttt
     1621 ttgtgatcgg cttagtggac aatgtcctgc gtcagattct cgtcggcaaa gacaccaaaa
     1681 tgcccgacta tgtggttctc atcgcaaccc ttggaggcat ggcgatcttt ggcatccatg
     1741 gctttgtcat cggtccggta atcgccgcga tgtttatggc cgtatgggat attctcgcta
     1801 tgaccaatca agccaggcaa cagaacgagc ccgcctgagt ctttcctaaa ctcctctcgc
     1861 ttggaacttc agagtcggcg cgccctacga tttactgcaa gctgaccatc gcaggagcag
     1921 tttcgagcac tggcaatctc aatgctcgcg caaagagcga gacactcgaa actccggcaa
     1981 atccgatttc tttcgacttg atttatcgta tcggcggcgt aaggtcgccg aaaccttctc
     2041 atgctggcca tcggtctcgc cctgatcgca tccttctgct ttgcagcagc gatgattctc
     2101 atcaatcgcg gcgtgctcgt gctcgattac tttcgcggtc tcctgaccaa tctcggtatc
     2161 aacgcgctct ttctgtgggt ttatttgttt tttgtgggaa accgccaggg gctttcggac
     2221 cgagccaatc ttatttttgt tttggtcggg gtttttgtgc cgggcgtggc gcgatttttc
     2281 atattcaaag gaatggagcg cctcggcgcc tcgatcacgg catgtctcac caatagcacg
     2341 cccttgtttg cgaccttgtt cgcggtgttt tttcttcgcg aacgaccgac ggtaacgaat
     2401 ctcttgggca cctttgcgat cgtctgcggt atcgttgcgc tctcatggaa aggctccaca
     2461 aagagttggc gcacgcgcga tctcctattt cccctcgcag cggctttttt gtttgcgggc
     2521 cgagacaact tggtccgctt cggcttgctt caaatcggct cgccggttct cggtgcggca
     2581 atcgctgcaa ccacatcgtt tctgacgatg tcggctcttt atatcgcctt cgaaaaaaaa
     2641 ttgcccctcg cctccagcgc agt
//
LOCUS       DASULZ010000425         2546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_965913, whole genome shotgun sequence.
ACCESSION   DASULZ010000425 DASULZ010000000
VERSION     DASULZ010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965913"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..968)
                     /locus_tag="VFY96_08630"
     CDS             complement(12..968)
                     /locus_tag="VFY96_08630"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5606566.1"
                     /translation="MIIDFEHHYIPAELGRRLGIDPTRREAVKTRDATVHAQLFDLEA
                     QLRDMDRVGIDVAVQSCILGWDTTLENSRLINDCTAAAQNRYAGRFVGLAHAPILEQG
                     GLRELERAIGELGLKGVTISSQVNGLPVDAKEFGAFYELMRRLRVPIFVHPALAPKGY
                     SLMNDYQLAVILTREFDLGLAVTRLIAGGVVERYPDLNFVFAHFGGGLAGYKERIARS
                     SYRFKLPQRFEEYFDRLYFDMAGFEGSSIALRCALEAIRPERMIFATDYPQNFNNSDP
                     KHGKNVDGVREYIDEIRNLPLDPKVKNDMLGGTAAKLLKIGV"
     gene            complement(993..1862)
                     /locus_tag="VFY96_08635"
     CDS             complement(993..1862)
                     /locus_tag="VFY96_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEX5606567.1"
                     /translation="MPVTEQKFTPAIMVHGGAGPIKDGSLPERLQGCKDAALVGWEIL
                     ERGGSSLDAVEAAVVALEDNPLFNAGTGSTLNSLGTVEMDAAIMEGESLRAGAVAAVS
                     GIKNPIKLARRIMEDGRHVMLAGDGARIFARQIGFAEVPAESLISERERNRWQSAHGT
                     VGCVAFDREGKLAVATSTGGIFNKLPGRVGDSALLGCGTYANKYGAVSCTGHGEAIMR
                     IVMARTALEYLQDGIEPFEAARCAVEDLERLTGGTGGLILIDPCGRVAYARNTSHMPI
                     CSITHAGDISIVA"
     gene            complement(2025..>2546)
                     /locus_tag="VFY96_08640"
     CDS             complement(2025..>2546)
                     /locus_tag="VFY96_08640"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX5606568.1"
                     /translation="ANNSSSVHTSVHAGTHVDAPFHFVPDGITIESLPLETFIGPARV
                     CGVEAGSHITALDVERANLAGETRVLFKTRNSNLLKKAEYDAGFAPFSVDAAKALVKL
                     GVRLVGLDYLSAAGANEQVPVHRAFLDHGVILLEGVDLSEVAPGRYELFCPPVKLSGS
                     DGAPCRAVLRELA"
BASE COUNT          563 a          794 c          673 g          516 t
ORIGIN      
        1 gtagatacga cttaaacgcc tattttaagc aattttgccg cagtaccgcc gagcatgtcg
       61 tttttgactt ttgggtcgag cggcaaattg cgaatctcat caatatactc gcgcacgccg
      121 tcaacgttct ttccatgttt cggatcgctg ttgttgaaat tctgcgggta atcggtcgca
      181 aagatcatcc gttcgggacg gatcgcctcg agcgcgcaac gcaacgcgat cgaactgcct
      241 tcgaatcccg ccatatcgaa gtaaagccga tcaaaatact cctcgaaccg ctggggcagt
      301 ttgaagcgat acgatgagcg cgcgatccgt tctttgtacc cggccaatcc accgccaaaa
      361 tgtgcaaaca cgaaattgag gtccggatag cgctccacca caccgccggc aatgagtcgc
      421 gtgaccgcga gaccgagatc gaattcgcgc gtcaggatca ccgcgagctg ataatcattc
      481 atcaaactat agcccttcgg cgccaaggcg ggatggacga agatcggtac tcgtaagcgc
      541 cgcattaact cgtaaaacgc gccgaactcc tttgcatcaa cgggcaaccc gttcacttgc
      601 gaggagatcg ttacaccttt caagcccagc tcgccgatgg cgcgctccaa ttctcgcaat
      661 ccgccttgtt cgagtatagg cgcgtgcgct aaaccgacga accggccagc atagcggttt
      721 tgcgccgccg cggtgcagtc gttgatgagc cgggagttct ccagtgtcgt atcccagccg
      781 agaatacagc tctgcacggc gacgtcgatg ccgaccctgt ccatgtcacg gagctgcgct
      841 tcgagatcga agagttgcgc gtgcaccgtc gcatcgcgcg tcttcacggc ttctctgcgc
      901 gtcggatcga tacccaaacg gcgcccaagc tccgccggaa tatagtgatg ttcgaagtcg
      961 atgatcatct cgcttgctct ttagagtcac gatcaagcta cgatcgaaat gtctccggcg
     1021 tgagtgatcg agcatatcgg catatgtgag gtgttgcgag cgtacgcaac ccgcccgcag
     1081 gggtcgatca gaatgagccc gccggtgccg ccagtgagcc gctccagatc ttcgaccgcg
     1141 caccgtgcgg cttcaaacgg ctcgataccg tcctgcaaat attccagggc tgtgcgtgcc
     1201 atcacgatac gcataatcgc ttcgccatgc ccggtgcacg agaccgcgcc atacttgttg
     1261 gcatacgttc cgcacccgag cagcgctgaa tcaccgaccc ggcccggcag cttgttgaag
     1321 atgccgccgg tcgaagtcgc taccgccagc ttgccttcgc gatcgaacgc gacacagccc
     1381 accgtgccgt gcgcgctttg ccagcgattt ctctctcgct ctgaaatcag ggactccgca
     1441 ggaacttcag caaagccgat ttgccgagca aagatgcgcg ctccatcgcc cgcaagcatt
     1501 acatggcgcc catcctccat gattcggcgc gcgagcttga tcggattttt aatcccgctc
     1561 acggcggcca ccgcaccggc acgcagcgac tccccttcca taatcgcggc gtccatctcg
     1621 actgttccga ggctattcaa ggtggaaccc gtcccagcgt taaacagcgg attgtcttcc
     1681 aatgccacga ccgccgcttc gaccgcatcc aatgaagaac cgcctcgttc gagtatctcc
     1741 cagcccacaa gagccgcgtc tttacagccc tgaagacgct ctggaagact accatctttg
     1801 atcggcccag ctccgccatg gaccatgatg gctggagtga atttctgctc ggtaacaggc
     1861 attctcctcc taactcaaag gtcgcggcgc cgttgtcatg tcgaccgaag ggagaaaacc
     1921 ttcttagaac ccgcgacgat gaacagacaa gcggcgcagc cggcgggaaa ttttgaactg
     1981 tgatcctcgt gatcgtctga ttcgctgaga attaatcgcc agcctcatgc aagctcgcgc
     2041 agcacggctc gacaaggcgc gccatcggag ccggagagct ttacaggcgg acagaataac
     2101 tcataccgac ccggcgccac ttccgataga tcaacgcctt cgagcaaaat gacgccgtga
     2161 tcgagaaatg cacgatgcac cggcacttgc tcgttggcgc ccgccgccga gagatagtcg
     2221 agaccgacga gcctaacacc gagcttcacc agcgcttttg ccgcatcgac ggaaaaaggc
     2281 gcaaatccgg cgtcgtactc ggctttcttg aggagattgg aattacgtgt tttgaacagc
     2341 acccgagttt cgccagccaa gttcgctctt tcgacatcaa gcgcagtgat gtggctgccg
     2401 gcctccacgc cgcagacccg tgccggcccg ataaacgtct ccagcggcag tgactcgatc
     2461 gtgatcccat cgggcacgaa gtgaaacggc gcatccacgt gagtacccgc atgaacactg
     2521 gtatgaaccg atgatgaatt attggc
//
LOCUS       DASULZ010000426         2299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_53504, whole genome shotgun sequence.
ACCESSION   DASULZ010000426 DASULZ010000000
VERSION     DASULZ010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53504"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VFY96_08645"
     CDS             <1..546
                     /locus_tag="VFY96_08645"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5606569.1"
                     /translation="QELARAEGKDGLDWFLDISLEEELETRFVHTNTQGDPNAVCEIL
                     KHPAVMIGQSDAGAHMGYDARFGYSTAFLGCWVRDHGIMSLEEAVNKLTFRVASVFGL
                     SDRGLLRPGFAADIAVFDPATVNTLEPEYVQDLPANETRMIQRAAGVPHTIVNGEVVI
                     ENGVPTGALPGKVLRPNGWRK"
     gene            899..1768
                     /locus_tag="VFY96_08650"
     CDS             899..1768
                     /locus_tag="VFY96_08650"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX5606570.1"
                     /translation="MEAMPSAVIAAANAKSRRLRELVRAPEILVMPGAYDVLSALLFE
                     QMGFKAIQGTSGGIAAALGYADGEVMSRDRFIEASGSFAAAVSVPFNADGERGYGDAN
                     GVRETVHALVARGVAGMNLEDGAAKGKGGLVELSEQLHKIKALMETKRELGSEFFLNA
                     RVDAFHVFPDDPKKALDEAIRRGNAYAEAGGDCIFYLFLHSAEIIGRIAKEVKAPISI
                     LAGPQSPSVSQLQDLGVARVSYGSGFLKAAITGAKRLAEEILEKGTCDLLKQGVQTPE
                     ISALVARRKKPIS"
     gene            1765..2238
                     /locus_tag="VFY96_08655"
     CDS             1765..2238
                     /locus_tag="VFY96_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009367031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX5606571.1"
                     /translation="MNRQGFVVSLHIAPTGAGTIQNHDQVEAIAGRGLEGDRYFAGIG
                     TYSHDPGSGRHVTLIEIEAIEGLKRDYGIDIAPGRSRRNIVTRNIALNHLVDRDFLIG
                     KVRLHATRLCEPCAHLEQLTEHGVLRGLVHRGGLRAEIVSGGTIYVGDSITLPDD"
BASE COUNT          501 a          621 c          695 g          482 t
ORIGIN      
        1 caggaactgg cccgggccga gggaaaagat ggtttggatt ggttcttgga catatccctc
       61 gaagaagagc tcgaaacccg ttttgttcat accaacaccc aaggcgaccc gaacgcggtt
      121 tgcgaaattc tcaaacatcc cgccgtgatg atcggacaat ccgatgctgg cgctcatatg
      181 ggctacgacg ctcgcttcgg gtattcgacg gcgtttctcg gttgctgggt gcgcgaccac
      241 ggtatcatga gcttggaaga agcagtcaac aaactaactt tccgcgtggc gtcggtgttc
      301 ggattgagtg atcgcggcct attgcgcccc gggtttgcag cggatatcgc ggtgttcgat
      361 cccgcgaccg tgaacacgct cgaaccggaa tacgtgcagg atctgccggc aaacgaaacc
      421 cgaatgatcc aaagagctgc gggcgtgccg catacgattg tgaacggcga agtcgtcatc
      481 gaaaacggcg tgccgaccgg agctttgccg ggaaaggttc tgcgcccgaa cggctggcgg
      541 aaatagggtt tgcaagaacc gctgcgcaga agaaccttgg ttgctcaatc gaaaatcgtt
      601 cgactagagc acgcacgccc gatccagtta tgacaaggcg aatggtcgtc taagaataac
      661 ttcgtttctg accgtcaaga gagatttccg aggcgagcgt cttttggacg cgattgcgac
      721 attgtccagc tgaatctttt caattctcct cgattcaaaa ccttgttttt cctgcgcgag
      781 ggcagtacgc cattcggaaa gtggtctttc gtttgacgtg cagcgcagcc gcttcctttc
      841 cttccatccc attgagtctt acgatacgat ccactgatca gatgagctgg aggttatgat
      901 ggaagcgatg ccaagcgcgg taatcgccgc tgccaatgcg aagagccgta gactcagaga
      961 attggtgcgc gcgccggaaa ttcttgtcat gcccggcgcc tatgatgtgt tgagcgcgtt
     1021 attgttcgaa cagatgggat tcaaagcgat ccagggcacg agcggcggca tcgcggcggc
     1081 attgggctat gccgacggcg aagtgatgag ccgcgatcgt ttcatcgaag cgagcggcag
     1141 cttcgctgcc gccgtgagcg tgccgttcaa cgctgacggt gaacgaggtt acggtgatgc
     1201 gaacggcgtt cgtgaaacgg tccatgcgct ggtcgcgcgc ggcgtcgcag gcatgaatct
     1261 cgaagatggc gcagccaaag ggaaaggcgg tttggtggag ctttctgagc agctccacaa
     1321 gatcaaagcc ctcatggaga ccaagcgcga gctgggcagc gaattttttc tgaacgctag
     1381 ggtggacgcc tttcatgttt ttccggacga tccaaaaaaa gcgctcgatg aggcgatccg
     1441 ccgcggcaac gcctacgctg aagcgggcgg cgattgtatt ttttacttgt tcctccattc
     1501 agccgaaatc atcggacgca tcgccaaaga agttaaagcg ccgatcagca ttctcgccgg
     1561 gccgcagtcg ccaagcgtga gccagctaca ggacttgggc gtagcgcgcg tgagctacgg
     1621 ctcgggcttt ctgaaagcgg cgatcacggg agcgaagcgc ctggccgaag agattctcga
     1681 gaagggcacc tgcgatttgc tcaaacaggg cgtgcaaacg ccggaaatta gcgcgctcgt
     1741 tgcccgccgc aaaaaaccga tctcatgaac cggcaaggct ttgtcgtttc gcttcacatc
     1801 gcgccgaccg gcgccggtac gatccagaat cacgatcaag tcgaagcgat cgccggacgg
     1861 ggacttgaag gtgatcgata ttttgccggc atcggcacct attcgcatga tcccggcagc
     1921 ggccggcacg tgaccttaat tgagatcgaa gcgatcgaag gcctcaagcg ggactacggc
     1981 atcgacatcg ccccgggtag gtcgcgccgc aatattgtga cgcgtaacat tgcactcaat
     2041 cacctggtcg atcgggattt tctcatcggt aaagtgcggt tgcacgccac gcgactttgc
     2101 gagccctgcg cgcacctgga gcaattgacc gagcacggcg tgctgcgcgg cttggttcat
     2161 cgcggcggtc tgcgtgccga aatcgtcagc ggcggaacca tttacgtcgg tgattcgatt
     2221 acgctgccgg atgattagcg ccgtcgaggt agtgcgagcg tgcctaatcg ggcgcagcaa
     2281 gtttaccgca attcaactt
//
LOCUS       DASULZ010000427         4793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1270236, whole genome shotgun sequence.
ACCESSION   DASULZ010000427 DASULZ010000000
VERSION     DASULZ010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270236"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..673)
                     /locus_tag="VFY96_08660"
     CDS             complement(167..673)
                     /locus_tag="VFY96_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606572.1"
                     /translation="MNSVAEARSRVRQWSELHPAAVLYDDDSAVILDVASGKSVRLSW
                     IDVTGFEEKIHPETQEPYLILLFEQGEQIALVDPGGVAFAPSTINSGPVEKLPPVVCL
                     RDFFTLKQRVDHYLYDHPGEAPPRECLDLVMICIAILDGARAVGFEVGDFEGELQKSL
                     DELERRTG"
     gene            793..2100
                     /locus_tag="VFY96_08665"
     CDS             793..2100
                     /locus_tag="VFY96_08665"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX5606573.1"
                     /translation="MNHSDSEDKTALYLLWAYCVFILYGCFIPFHFNFDPNFVRWRWV
                     VFVTESLHEDIARPSLSDIVSNILLFVPFGILSVWARRADRTDRAGFLAILSTTVYGL
                     LFAIIIESGQTVAPGRDPSIRDAACNGFGAFIGAVCATVFIRDYRKYLQSEFIRVLFR
                     TPSLLLLAYLICGILLDSYFPFEVTLDVSSVWHNLKNSQILPFRSTSDAYWLNLWVDK
                     GIPYGVIAYLFLTNLRPSSRLLTVGLAWLLTIVMACAIELGKLFFAGRAFYSDNVAIA
                     SVGALLGVLLLPRFCAVASVKRHREAIWFMLFLGFLVYFELSPFDWVAPSELRGRFSA
                     IEWLPFKSYYYGEPIAALFDLQKKVYSFIPFGFFAAGFVSAHAPGRSRQKTASLCFLV
                     AAGLELFQIGLRSRIPSTGDVIIFSLSAWAGTVLFDLINSVQR"
     gene            2401..2859
                     /locus_tag="VFY96_08670"
     CDS             2401..2859
                     /locus_tag="VFY96_08670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX5606574.1"
                     /translation="MRKLILALIMCLALAGTAAAVVNINTASKEELTTIKGIGDKRAQ
                     EIIDYRKKNGDFKSVDELEKVPGIGPGTMKQIRSQVTVSGKTTTDKPADKGTKTKTSD
                     VKKSETSKADSMKSDTAKAKAASDNPKGDSMKADTAKAKTAEKSKADEKK"
     gene            3081..4205
                     /locus_tag="VFY96_08675"
     CDS             3081..4205
                     /locus_tag="VFY96_08675"
                     /inference="COORDINATES: protein motif:HMM:NF021508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HEX5606575.1"
                     /translation="MAKTWIVCIDGTWNQPGQRDKDPVTAQEEAAPSNVARTWEALAN
                     TPLVTEFYYGSIAPIKPKILFTGVEGEVIYLAGIGTSGTVNTHILEGATGTGTSERIR
                     DAYRYLAERYRPGDRIYGFGFSRGAFAIRSLAGLIDLAGLPKQPRALKEEELLQIYDA
                     YMDGKLIDKARYGTVDTMFDFLGLWDTVGALAFGRTLGNFHRINPGNVKCVAHALALD
                     EERERFELAFWDPPATATTQVDEVWFTGCHTNIGGGYCDTNLSNIALFWMLQSARDAG
                     LPLDSRGIPGFDVKDPRGLQRDSYKEFYDCMGLIGSIAESLNLKRQPRTIRQGQRIHQ
                     SVFDRMQEAPGQQLYVPKALFHGKPLSAEFNPGIQPWGGF"
     gene            4243..4575
                     /locus_tag="VFY96_08680"
     CDS             4243..4575
                     /locus_tag="VFY96_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX5606576.1"
                     /translation="MENSVKKFEDLIAWQKARELTRRVYEITRTREFAKDLRLSGQIQ
                     SAAVSIMSNIAEGFERSGLGEFHQFLSTAKELRSQLYVALDVRYIDERTFQTCGSRQK
                     RSAELLAD"
BASE COUNT         1093 a         1286 c         1233 g         1181 t
ORIGIN      
        1 gataccgatt catggccgca tttggggcaa tataacctgc tgacaattcc catggaggct
       61 ctgagtctga gtcgttaatg aactcgtgcg atgtcgtcgt tcgaaacaga tccttcggct
      121 ttgcctcagg atcaccattc accgcgggtc tccttacttt aaattcttat cctgtccgtc
      181 tctccaattc gtcgagggat ttttgcagct cgccctcgaa atcgccaact tcgaatccca
      241 cagcgcgtgc gccatccagt atggcaatgc agatcatgac caaatccaaa cactcccgcg
      301 gcggcgcctc gcccggatga tcgtacaggt aatggtcgac tcgctgcttt agcgtaaaaa
      361 agtctctgag acaaaccacc ggcggcagct tttccaccgg ccctgaattg atcgtgcttg
      421 gcgcaaacgc cacgccgccc ggatcaacaa gcgcgatttg ctcaccctgt tcaaacagca
      481 agattaaata cggctcctgg gtctcgggat ggattttttc ttcaaacccg gttacatcga
      541 tccatgacag ccgcaccgat ttgcccgagg caacgtcgag aataaccgcg gagtcatcat
      601 cgtagagtac cgcggctggg tggagttcac tccattgccg cacccttgac ctagcttcag
      661 cgacgctatt catcgaatca gcctagcatg tggcattgct cgacctaata actcgctcaa
      721 cgaaactaac tttggccgcc tcaaagtgat gatccgcacg cgcaaagaat acgataaacc
      781 tcaagagatt gcatgaatca ttccgattca gaagataaaa cagcgctcta cctactctgg
      841 gcatactgcg tgttcattct ttacggctgt tttattccgt tccatttcaa tttcgatcca
      901 aatttcgtgc gctggcgctg ggtcgttttc gtcacagaat cgctgcacga ggacattgcc
      961 agaccttccc tatccgacat cgtcagcaat attcttcttt ttgtgccgtt cggtatcctt
     1021 tcggtctggg cccgcagagc cgatcgtaca gatcgcgcgg gatttctagc gattctatcg
     1081 acgacggtat acgggcttct attcgcgatc atcatcgagt ccggccaaac tgtggcaccc
     1141 ggaagagatc cgtcaatacg cgacgccgcg tgcaatgggt tcggtgcttt catcggcgca
     1201 gtctgtgcta ctgtgtttat ccgagattat cggaaatatc tccaaagcga attcatccgg
     1261 gtcttattca gaacgccgtc gttgcttctg cttgcctact taatttgcgg aattttgctg
     1321 gactcctatt ttccatttga agtgacatta gatgtctctt ctgtgtggca taacttaaaa
     1381 aacagccaaa tccttccgtt tcggtcaacg tctgacgcat attggcttaa cctctgggtc
     1441 gacaaaggta ttccatatgg cgtgattgcc tatctgtttt taaccaatct gcgaccgtcc
     1501 tcgcgcctgc tcacggtggg tttggcgtgg ctcctgacca ttgtaatggc ctgcgccatc
     1561 gagttgggaa aactcttttt cgccgggaga gcgttttatt ctgataatgt agcgatcgcg
     1621 tcggtcggcg cgctcctagg tgtcctcttg cttcctcgct tctgtgccgt cgcctcggta
     1681 aagcgccatc gggaagcgat ttggttcatg ctcttcctcg ggtttctagt ttattttgaa
     1741 ctgtcgccct ttgactgggt cgcaccgagt gagctgcggg ggcgattttc cgcgatcgag
     1801 tggctgcctt tcaaatcata ctattatggt gaaccgatag cggctttgtt tgaccttcag
     1861 aagaaagtgt attcgtttat tccattcggg ttttttgcgg cggggtttgt ctctgcccat
     1921 gctcctggac gctcgcggca aaagacggca tcactgtgct ttttggttgc cgccggtctg
     1981 gagctttttc agatcggctt acgatcgagg attccttcaa ccggcgacgt gatcatcttt
     2041 agtcttagcg cttgggccgg cactgttctt ttcgatttga tcaattccgt ccagcggtga
     2101 ctagtgcaca atcgattgcc tttgattgtt gaatcattgg ggatacagtt catataccca
     2161 ttgaaatgaa acactcaatt ttcctgtcat ctccgcgaac tgagggagct tgaccggacc
     2221 gcatccctac gaggcgttgt gggtggtgta cggctatcct ttcgccaggc gaacttgaca
     2281 gccgctgacc ctcttcgcta tagccgagtc gaagctcgct gctcggtggc aatccatacg
     2341 agtgaatgat tggcttcacc cttcgtctaa agagtaatcg aatacaccaa aggaggactt
     2401 atgagaaaat taattttggc tcttatcatg tgcttggcac ttgcgggaac cgccgccgcc
     2461 gtggtcaaca tcaacacggc gagcaaggaa gaactcacca cgataaaggg catcggcgac
     2521 aagcgcgcgc aggagattat cgattatcga aagaaaaacg gtgacttcaa gtccgtcgat
     2581 gaactggaaa aagtgcctgg aatcggcccc ggcacgatga agcaaatccg ctctcaggtt
     2641 acggtcagcg gcaaaacaac gacggataag ccggctgaca aggggacgaa aaccaaaacc
     2701 tccgacgtaa agaagtccga aacctccaaa gccgattcaa tgaagtcaga caccgccaag
     2761 gcaaaggctg catcggacaa tcctaagggc gattcgatga aagcagatac cgcaaaagca
     2821 aagactgccg aaaagtccaa ggccgacgaa aagaagtaac ccaccctatt cactacataa
     2881 acccgccggt gttttaccgg cgggttttgt ctttggcgca ctgacgccat actgacccga
     2941 gaacttctag ttgcgctaaa ttcgtaccca tccgcttcgc ctttcgaagt ttcgctaact
     3001 ttttactgct ctcgtcgggg tatgttagaa tctcactcaa cgtacatacc ctcacggaga
     3061 tcttgattgc ggggagaagt atggcaaaga cctggatcgt ctgcatcgac ggcacctgga
     3121 atcagccggg ccaaagagac aaggacccgg tcactgccca ggaggaagct gcaccatcga
     3181 acgtcgcgcg cacctgggaa gcgctcgcga acacgccatt ggtcacggaa ttttactacg
     3241 gctcgatcgc tccgataaaa ccaaaaattc tttttaccgg cgtggaagga gaagtcatct
     3301 atcttgccgg cattggcacc agcggcaccg taaacacgca catccttgag ggagcaacgg
     3361 gtaccggcac ctctgaacgc atccgggatg cctaccggta tctggcggag cggtatcgtc
     3421 ccggcgatcg tatttacggt tttggtttta gtcgcggcgc cttcgccatc cgcagtctag
     3481 ccggtttgat cgacctcgcc ggtctgccga aacagccgcg cgctctcaag gaagaggagt
     3541 tgctacaaat ctacgacgct tacatggacg gcaagcttat cgataaagcc cgctacggca
     3601 ctgtcgatac gatgttcgac tttcttggtt tgtgggacac ggtcggcgcg ctcgcgtttg
     3661 gccgcacact cggcaatttc catcggatca atccgggcaa cgtgaaatgc gtcgcgcacg
     3721 ccttggcgct tgatgaggaa cgcgaacgct tcgagcttgc gttttgggat ccgcccgcga
     3781 ccgcgacaac tcaggtcgat gaggtatggt tcaccggctg ccatacgaat atcggcggtg
     3841 gctactgcga taccaatcta tcgaacatcg cattgttctg gatgttgcaa tcggcgcgcg
     3901 atgccggatt gcctctcgat tcacgcggca ttcccggctt cgatgtgaaa gatccgcgcg
     3961 gacttcagcg tgattcgtac aaagaattct acgactgcat gggcttgatc ggcagcattg
     4021 ccgagtcttt aaatcttaaa cggcaacccc ggacgatccg gcagggacag cgaattcatc
     4081 aaagcgtgtt tgatcggatg caagaagcgc cgggccagca gctgtacgtt ccgaaggcgc
     4141 tgtttcacgg caagccattg tcggccgaat ttaatcccgg cattcagccg tgggggggat
     4201 tttgatagtc gcgagtgctg agtcctgagt gctgagaatc ggatggaaaa tagcgttaag
     4261 aaatttgaag acttgattgc atggcaaaaa gcccgcgagc tgacacgcag ggtttatgag
     4321 atcacccgca cgagagagtt cgcgaaagac cttcggctat ccggccaaat tcaatctgcc
     4381 gccgtttcaa ttatgtccaa catcgcagaa ggatttgaaa ggagcggctt gggtgagttt
     4441 catcaatttt tatcgacagc aaaagaactg cgttcgcagc tctacgtagc actagatgtc
     4501 cggtacatcg atgagcgtac ctttcaaacc tgcggctcca ggcagaagag gtcggcagaa
     4561 ttattggcgg actgagagtc tcagtcgcgc agcaacgaaa gaacagatcg agttgacttt
     4621 cctcggcact caggactcac cactcagcac ttcccatagc agttccgcag gatgtttggc
     4681 tttgcgatgg gcgagatgct cgatttgctg tcggcacgag attcctggcg cgacaatgtt
     4741 gatttcgggt ccgcttgcat ttacggcagg cactaaacgc cggttagcga tct
//
LOCUS       DASULZ010000428         3008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_864884, whole genome shotgun sequence.
ACCESSION   DASULZ010000428 DASULZ010000000
VERSION     DASULZ010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864884"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..603)
                     /locus_tag="VFY96_08685"
     CDS             complement(49..603)
                     /locus_tag="VFY96_08685"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5606577.1"
                     /translation="MAVNVEKLKSLTPPRGMDFHISKVGHVVLQVSDLERSTEFYTQV
                     LGFKVSDVYPEDMMPGGMVFLRCGTDHHCLALVGAGDGKNAHRELHHLAFEVPTLAEV
                     LHARDRLRQCGAKIDFEGRRRAGCQIAVEFRDPDNNSLEIYWGVDQVGSDGRVRPANE
                     WHGVKSLDEAIANPVPGQDTHIPK"
     gene            complement(764..1567)
                     /locus_tag="VFY96_08690"
     CDS             complement(764..1567)
                     /locus_tag="VFY96_08690"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5606578.1"
                     /translation="MPYSTANGLKIYYEVSGEGLPFVMVHANPFDHNLWMYQIAHFST
                     YFKVVAIDIRGYGRSDKPSAPFTLKDMADDVLGVCRDERINEAILGGVSVGSGMAILL
                     GLDHPEMFKALILVGGSSGPGASIEDRIHGYTKIGIEKYHIQHLRELVAPEFPESKLG
                     NYLLQTFVERDPWLSGESIGQIFRARGGLDMTPRLRTMRVPTLVINGEYDNSLAGGRK
                     TASLIPGAMHKIVPKTGHACNIEDPATFDAFAIEFLRANGLMPEQPTRS"
     gene            complement(1692..2396)
                     /locus_tag="VFY96_08695"
     CDS             complement(1692..2396)
                     /locus_tag="VFY96_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX5606579.1"
                     /translation="MSFDFEDHCWKDIVTPEILKTYIPYQRETFIGQRPALLAIDLYN
                     LAYQGGAKEPHELVDTYKSSCGVYAHNAIAPTQELFALARSLKLPVFYTTTETRQEAK
                     PGAVHATNRQRVKIDPQAFEIKEEFKPETGDVVIFKERASGFFGTPLVAHLTQLGVDS
                     LIVCGESTSGCVRASCVDAYSLGYHVTIVEECVFDRSPLSHKVNLFDMHHKYTDVMKL
                     EEVKEKIKQERPRKAN"
     gene            2815..>3008
                     /locus_tag="VFY96_08700"
     CDS             2815..>3008
                     /locus_tag="VFY96_08700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX5606580.1"
                     /translation="MAKNSNNPKLHGRSPMLSRRQFLKLAAATTAAAGTASWSRARVF
                     AAQPARKRIVIHGERQVTSLG"
BASE COUNT          683 a          792 c          833 g          700 t
ORIGIN      
        1 ctgcggctca gaatgacatt cgtgactggg acaacggttg tgacggcgtc actttggaat
       61 atgcgtatcc tgtcccggca ccggattggc gatcgcttcg tcgagggatt taacgccgtg
      121 ccattcgttt gccggccgta cgcggccgtc actcccgacc tggtcgacac cccagtaaat
      181 ttccagggag ttattgtccg gatcgcgaaa ttccaccgcg atttggcagc ccgcgcggcg
      241 ccggccctcg aagtcgatct tggcgccgca ttgacgcaag cgatcgcgcg cgtgcaacac
      301 ttccgccaaa gtgggaactt cgaaagcaag atgatgcaat tccctgtggg cgtttttgcc
      361 gtctccggcg cccaccaatg ccaagcaatg atgatcggta ccacagcgca aaaaaaccat
      421 gccgccaggc atcatgtctt caggataaac atctgatact ttgaacccga gaacctgagt
      481 ataaaattcg gtggatcgtt ccagatcgct tacctgtaag actacatggc ccactttgct
      541 gatatgaaaa tccatccccc gcggcggtgt gaggctcttc agtttttcca cattgacggc
      601 cataaagagt ctcctttatc aattcgaacg gtcaattcta taacatttcc ggtgtagttc
      661 tgcacatgcg gagcattaaa atccgatgct tggctgccgc tcagcacgac acctctgttg
      721 atagatcgct gggctgcgct cgcacaaata aagacgcggg tatttaagag cgagttggct
      781 gttcaggcat caagccgttg gcgcgcagaa attcaatcgc gaaggcatcg aatgtcgcgg
      841 ggtcttcgat gttacaggcg tgtcccgtct ttggaacgat cttgtgcatg gcgccgggaa
      901 tcagcgaagc agtttttcta ccgccggcga gtgagttgtc gtattcaccg ttgatcacca
      961 aggttgggac tctcatcgtg cggagccgcg gcgtcatatc aagcccgcca cgggcgcgga
     1021 aaatctggcc aatggactcc ccggaaagcc atggatcgcg ttcgacgaat gtctgcagca
     1081 gataattgcc aagcttgctt tccggaaact caggagcgac cagctcacgg agatgctgaa
     1141 tatggtattt ctcgatacca atttttgtgt agccgtggat tctgtcttcg atggaagcgc
     1201 cgggaccgct actgccgccg actaaaataa gcgctttgaa catctccggg tgatcgagtc
     1261 ccaacaaaat agccatgccc gagccgacgc tcacgccgcc caaaatcgct tcgttgatgc
     1321 gttcatcacg gcaaacgccg agcacatcgt ccgccatgtc cttaagggta aaaggcgcgc
     1381 tcggcttgtc cgagcggccg tagcctcgaa tgtcaatggc gacgactttg aaatatgtcg
     1441 agaagtgcgc gatttgatac atccatagat tatggtcgaa cggattggca tgaaccataa
     1501 cgaagggcaa accttcgccg gaaacttcgt aatagatttt cagaccatta gcggtgctgt
     1561 agggcatggc aaaaggtcgt tcaaactgtt caagctgttc aaaacgttaa aacgccggaa
     1621 cagggaaacg accaacaatt tgaacggagc gaagcgatcg aacggcttga acgatttgaa
     1681 cgagttcagg tttagttcgc ttttctcggc cgctcctgct tgattttctc tttgacttct
     1741 tcgagcttca tgacgtcggt gtatttgtga tgcatgtcga agagattgac cttgtgcgac
     1801 agcggactgc gatcgaatac gcactcttcg acaatggtaa cgtgatagcc gagcgaatag
     1861 gcgtcgacgc agctcgcgcg cacgcagccg ctagtgctct cgccgcagac gatcaggcta
     1921 tctacaccca gctgagtgag atgcgcaacg agcggcgtac cgaaaaaccc gctggcgcgt
     1981 tccttgaaaa tcacgacgtc gcccgtttcc ggtttgaact cttccttgat ctcgaacgct
     2041 tgcgggtcaa tcttcacgcg ctggcggttg gtcgcatgca ccgcgccggg tttggcttcc
     2101 tggcgggttt cagtcgtggt gtaaaacacc ggaagtttaa gagaacgcgc taaagcgaat
     2161 agctcttgcg tcggcgcgat ggcattgtgc gcatacactc cgcacgagct tttatatgtg
     2221 tcgaccagtt cgtgcggctc cttggcgccg ccctggtaag cgaggttgta aagatcgatc
     2281 gccaagagcg ccggcctttg accgataaaa gtctcgcgct ggtagggaat gtaggttttc
     2341 aagatctccg gcgtcacgat gtctttccag caatgatctt caaaatcgaa gctcatcaga
     2401 atctccttgc cgagtaaatg gttgcgtagc tatcattcat ctacccgcag ctaaccggag
     2461 ttgtcaagga acggatcggt cgggtgcgaa gcgcctatcc ttcgatattc accgttgaca
     2521 cactgcctac gcaggatgtt tagcctgatc cgttttccga gtagcccgaa gcgtgtatcg
     2581 agggataagc gagcgtgtcg gtcgctactc gggaaatcgg gcgcttggat gtgttttcct
     2641 ttcgacctgg caacaaataa atagcgttcg aagttcttga atgcgtgact aatgcttgca
     2701 ggttcaagta gtgaaccgaa caggaatctt ggagatggtg gatggattga cagaccaccg
     2761 cgcgtcgatt aaatggttgc gggcggattt ttgtggcggt gaaaggaaat tgtaatggcg
     2821 aagaatagca acaatcccaa gctgcacggg aggtcaccga tgctgagccg gcggcaattt
     2881 ttaaagctcg ccgccgccac gacggccgct gctggaacgg catcttggtc gcgagcacgc
     2941 gtcttcgcgg cgcagcctgc gcgcaagcgt atcgtgatcc acggcgagcg gcaggtgact
     3001 agccttgg
//
LOCUS       DASULZ010000429         4329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1066567, whole genome shotgun sequence.
ACCESSION   DASULZ010000429 DASULZ010000000
VERSION     DASULZ010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066567"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1539
                     /locus_tag="VFY96_08705"
     CDS             <1..1539
                     /locus_tag="VFY96_08705"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HEX5606581.1"
                     /translation="LDLRLAGNKIAIRNGHLKLHSLDLAAAGDIQTGNNTVLNLSFDS
                     KPASLEGWDRIVPALADYQLSGTMDVKATVRGSIGKGAAPHIQGTLGLKKVSAKPPSF
                     PKPIEDLDTTVRFSGQRANISDMTLSLGKSRIRLAAAIEKFAPLTLSYKMSTPELWPA
                     DYSTALTEERKADVIRNLQSAGQFSMSANGMFYEGKLSSTEGTLYNVPYKALDAALSL
                     ADKVANIKSLRVNALSGALQIDGQYSFKEAVPQFSMASKVQNIDVKELYTLLDAKAER
                     DIRGRMNADMKLAGSGKSWEAIKPTLHGRGDAEVVQGALLNFNIADNVLTGITGIPGL
                     TNAINPSLRAKYPETFTAKDTEFKELKANFDLADSRMNVKNLRMAAADFVVQGDGWAD
                     FDRKVDFRATLSFSQRLSADLSQSAREIKYLLNNQGQLEVPFALTGRMPNVKPKPDTR
                     YLGQMVQRGFLRKGTEELQNRFFGGGKDSGAQNEQTPSDTNKKKKTSTEDMIRRGLEG
                     LFKR"
     gene            complement(1602..2102)
                     /locus_tag="VFY96_08710"
     CDS             complement(1602..2102)
                     /locus_tag="VFY96_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606582.1"
                     /translation="MGFSLESFAADVKEVLQQDPSPAGRVRVRQLVEEALKDEKFIAS
                     YINDNTPDRQVIYEDPDLGFCVCAHVNRDAREANPHDHGSSWAIYGQAFGETEMSDWE
                     IVEPAAEGNPGKVRRARVYSLKPGMAHIYNEGDVHSPKRVAATGLLRIEGKNTQKMKR
                     LAYKAI"
     gene            complement(2253..2447)
                     /locus_tag="VFY96_08715"
     CDS             complement(2253..2447)
                     /locus_tag="VFY96_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HEX5606583.1"
                     /translation="MASRVKIDCRKYPSDKGCTVAISGAAEEVVELGWLHAKMHHAHK
                     DEEEKALKDWIRANAEPTND"
     gene            complement(2492..3130)
                     /locus_tag="VFY96_08720"
     CDS             complement(2492..3130)
                     /locus_tag="VFY96_08720"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEX5606584.1"
                     /translation="MFEIGGKQIAANLAEIVAPKHSALLIWDMEYAIGPNAFNYKDIL
                     ANLKGLSACAREVGVPVFYSVQTGFDLLKEEAPVWVRVRMKRAKASDPNQLLKEKDDP
                     HDREIVEELKPQPKDIVFQKRRPDGFIGTDLDLILRSNSVRTILIGGVATEGGIEGTA
                     RTARNLGYDIVVLKDCVGSRNRELHELALKLMEQTHFDISTAGEIAEIWRRR"
     gene            3280..4311
                     /locus_tag="VFY96_08725"
     CDS             3280..4311
                     /locus_tag="VFY96_08725"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5606585.1"
                     /translation="MIHTNFRRTVLMSAVFAITLTAQARAQDFFKDKTLIFIVGYSPG
                     GTYDQYTRLLARHIGKHVSGNPTRVVENMPGAGGIIAANHLYNRVKPDGLTIAAWASP
                     LILQQIMGNEATKFDARKVGWLGIPGPYDTACHFNQQSGIKSVDDWFTAKRPLKIASI
                     GPGTSLSDVPKLLKAALNLPLEMVEGYKGGAEARLAVESGEVDGLCASWQATKVSWRS
                     QMETGKIHVVLQATLKSHPELKKVPLAIDYAKTEEARTLLRVADNVHVYQFPFSTAPG
                     TPPDRLQVLQQAFVKTLRDPELINEAKKADLEIAPIDGPTTAKTFNSLYELPPTLIAK
                     LKELLIPKR"
BASE COUNT         1012 a         1175 c         1187 g          955 t
ORIGIN      
        1 ctggatctca ggcttgccgg caataaaatt gccatccgca acggccatct gaagctccac
       61 tcgttggatc ttgctgcggc cggagacatt cagacgggaa ataatacagt tcttaacctc
      121 tctttcgatt ccaagcctgc atccctggaa ggatgggatc ggatcgtccc tgcactcgcc
      181 gattaccaat tgtccggaac aatggatgtg aaagcgaccg tgcgcggcag catcggcaaa
      241 ggagccgcgc cacatattca ggggaccctc gggcttaaaa aagtgagtgc aaaaccaccg
      301 agttttccga aaccgataga ggaccttgac accaccgtca gattttctgg tcagagagcc
      361 aatatcagcg atatgaccct gagcctcgga aaatcgcgga ttcgtctcgc ggcggcgatc
      421 gagaaattcg ctccgctgac gctctcgtat aaaatgtcga cgccggagct ttggccggcg
      481 gattacagca ccgcgcttac ggaggagcgt aaagccgacg tgattcggaa cttacaaagc
      541 gcggggcaat tcagtatgtc cgcaaacgga atgttttatg agggcaagct cagctccacc
      601 gaaggcacgc tttacaatgt gccctacaaa gcgctcgatg cggcgctttc gctggcggat
      661 aaggtggcca atatcaagag tttacgcgtg aatgcgctga gcggcgcgtt acagatcgac
      721 ggccagtact cgttcaaaga agcggtgccg caattttcga tggcatcgaa ggttcaaaac
      781 atcgatgtga aagaacttta cacgcttctc gatgccaagg cggagcgcga catccgtggc
      841 aggatgaacg cggatatgaa gcttgccgga agcggcaaga gttgggaagc aatcaaaccc
      901 acattacatg gacggggtga tgccgaagtc gtgcaaggcg ctctactcaa tttcaatatt
      961 gccgacaacg tgctcaccgg aattaccggc atacctggac tgaccaacgc gatcaacccg
     1021 tcgttgagag caaaatatcc ggagactttc acggccaaag acacggagtt taaagagctt
     1081 aaagcgaatt tcgatttggc agacagtcga atgaacgtca aaaacttgcg catggccgcg
     1141 gcggacttcg tggttcaagg agatggctgg gcggatttcg atcgtaaggt cgattttcgc
     1201 gccacgttga gtttctcaca gcggctttcc gccgatctga gccaatcggc tcgcgagatc
     1261 aagtatcttc tcaataacca aggccagctc gaggtgccgt ttgcgttaac cggcaggatg
     1321 ccaaacgtca agcctaaacc ggatacgcgg tatcttggcc aaatggtgca gcggggtttt
     1381 ttgcggaaag gaaccgaaga gttgcagaac cgatttttcg gcggcggcaa agattccggg
     1441 gcgcagaatg aacaaacgcc gagcgacacc aacaaaaaga aaaaaacctc aaccgaagac
     1501 atgatccggc gcggccttga aggtttgttc aaacgctgag cggcgaaaac ttcggtggta
     1561 aatgtcgcgt tcggctttca gtcgattagg caggtgccga attagatagc cttgtacgcc
     1621 agccgtttca ttttttgcgt gttcttcccc tcaatgcgca gcagacccgt ggcggcaact
     1681 cgtttcggcg aatgcacatc gccttcattg taaatatggg ccattcccgg tttcagtgag
     1741 tagacgcgcg cccggcggac ctttccagga ttcccctccg cggccggttc gactatctcc
     1801 cagtcgctca tttccgtctc gccaaacgct tgaccatata tcgcccagga gctcccatga
     1861 tcatgcggat tggcttctcg ggcatcacgg tttacatgag cgcagacgca gaaccccagg
     1921 tcgggatcct cgtagatgac ctgccgatcc ggcgtattgt cattgatata actggcaata
     1981 aatttctcgt ccttgagagc ctcttcgacg agctggcgga ctctcacgcg ccccgcgggg
     2041 ctggggtctt gctgcagcac ctctttgaca tcggccgcaa acgattcgag agaaaaaccc
     2101 ataacaacgc tccttccgta attgagttct cagtcgtgtt tacaaaatag cgttagcgac
     2161 gtcaacttct aagcggcgct cacggcaagc taccagggat ggatcgagat ccctcggaaa
     2221 gaaatgtatt caaactctgt gctgagggat gctcaatcgt tcgtcggttc agcgttggct
     2281 ctgatccagt ccttgagcgc cttttcttct tcgtctttgt gggcatggtg catttttgcg
     2341 tggagccagc ccagctcgac cacttcttcc gcggcgccgg aaatggccac ggtgcagcct
     2401 ttatcactcg gatacttgcg acagtcaatt tttacacgac tagccatgaa aacctcctcg
     2461 gtattcaaag agcatactct ctgcgtacca attatcgccg ccgccagatc tccgcgatct
     2521 caccggcagt ggaaatatcg aaatgggttt gctccatcag ctttaacgcc agctcatgca
     2581 actcgcggtt gcgcgacccg acacagtctt tcaacaccac gatgtcatag ccgagattcc
     2641 gggcggttct agcagtgcct tcgatgccgc cttcggtggc gacgccgccg atcaggatcg
     2701 tgcgcacgct attgctgcgc aggatgaggt cgagatccgt accgatgaag ccgtccgggc
     2761 gccgtttctg gaagacgatg tccttcggtt gaggcttcaa ctcttcgaca atttcgcgat
     2821 catgcgggtc gtccttttcc ttcaacagct ggtttggatc ggatgccttg gcgcgcttca
     2881 tccggactcg tacccatacc ggagcttctt cctttagcag atcgaaaccg gtttgcactg
     2941 aatagaacac gggaacgccg acctcacggg cacaggcgga caagcctttc aggttcgcaa
     3001 ggatgtcttt gtagttgaaa gcgtttggtc cgatggcgta ttccatgtcc cagatcagca
     3061 gagccgagtg tttcggcgcg acgatttctg ccaaattggc ggcaatttgc tttccaccga
     3121 tttcaaacat gattaacctc tgcgacgctg gatttgcccg gagcatactc acatgcatgc
     3181 gcaggcgtca acggattggt ttcaacggag gacaattcgg tggaagcgcg ccaatggatg
     3241 tagtaggtat ctggttttga taaagccgag gagaagtcaa tgattcatac aaactttcgg
     3301 cgcacggtgc tgatgagcgc tgtttttgca atcactctca ccgcgcaagc gcgcgcgcag
     3361 gattttttca aagacaagac gttgattttt atcgtcgggt attcgccggg cggtacctac
     3421 gatcaatata cgcgcctgct cgcccggcat attggcaaac atgtgagcgg caaccccacg
     3481 cgagtcgtcg agaacatgcc tggggcgggt ggcatcatcg cagcgaatca tctttacaac
     3541 cgggtgaaac ctgacggtct cacgatcgcg gcttgggctt cgccgctgat cctacagcag
     3601 attatgggca acgaggccac caagttcgac gcgcgcaaag tcggttggct cggtattccc
     3661 ggtccctacg acacggcgtg tcatttcaac caacagagtg gaatcaagag cgtggacgat
     3721 tggtttacag ccaagcggcc tctcaagatc gcttcgattg ggccgggcac aagcctgtcc
     3781 gatgttccca aactgctcaa agccgcgctc aatctgccgc tggaaatggt tgaaggttac
     3841 aagggcggag ctgaagcacg cctggcggtc gagagcggcg aggtggatgg cctttgcgct
     3901 tcctggcaag cgacaaaagt atcctggcgt agccagatgg aaacgggcaa gattcacgtc
     3961 gtgctgcagg cgacgctaaa atcccatccc gagctcaaga aagtgccgct ggcgatcgat
     4021 tacgctaaga ctgaagaagc acgcaccctg cttcgagtcg ccgacaatgt gcacgtttac
     4081 cagtttcctt tctcaaccgc gccgggcacg ccgccggacc ggttgcaggt tttgcagcag
     4141 gctttcgtga aaacgttgcg tgatccggag ctcatcaacg aggcgaagaa agcggatctg
     4201 gagatcgcgc cgatcgacgg tccaacgacg gcgaagacgt tcaatagcct ctatgagctg
     4261 ccgccgacgc tgattgcaaa attgaaggag ctgctgattc cgaagcgcta aacgggaaaa
     4321 aaaggttca
//
LOCUS       DASULZ010000430         7905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_356840, whole genome shotgun sequence.
ACCESSION   DASULZ010000430 DASULZ010000000
VERSION     DASULZ010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7905
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356840"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VFY96_08730"
     CDS             <1..321
                     /locus_tag="VFY96_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX5606586.1"
                     /translation="VQEGLVLVNAMHITASVFINDDESGLHHDYTVWLEKLAPHEPLK
                     QYRHNDTGEDNADAHIKRQVMGREVVVAITGGKLDFGPWEQIFYGEFDGRRRKRVLVK
                     IIGD"
     gene            329..1561
                     /gene="rlmD"
                     /locus_tag="VFY96_08735"
     CDS             329..1561
                     /gene="rlmD"
                     /locus_tag="VFY96_08735"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEX5606587.1"
                     /translation="MARFYQRRDTEEHGRSAPIQSRPTYRPPCPHYPHCVGCPLIDVP
                     YPEQLARKRAIVLESFARYASLAAVEVPRLAPAPERLGYRTRVKLVARRNRDQIALGL
                     YVPSSHRVIDISSCPVHPRPVNQAAQYLKKKILELGIMPYDERDDSGDLRYVDFRFSA
                     ARQELSVTLVTRHSSFPAGVRLAHSLMKRFPFVTGVNQNVNEARGNVIWGPEFRTLAG
                     RETLLDRIGDLKLVLPPGVFSQANPSTARKLYEAVCNFADLSGRETVLDLYCGVGPIS
                     LYLGSTARQVWSVDDSELAVNTAKQNARRNGRGNCRFLAGDVASLATKLKNDLGDIDL
                     AVVNPPRKGVQPVAMKAIIECGAPKLIYVSCEPTTLARDLDRLVNGGYRIERLALFDM
                     FPQTEQVESVALLSKREV"
     gene            complement(1692..2951)
                     /locus_tag="VFY96_08740"
     CDS             complement(1692..2951)
                     /locus_tag="VFY96_08740"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5606588.1"
                     /translation="MSSPQETSLPESHPAISIRPWSSLLIRDYRLIFITILCANTSNN
                     MRNVASLYQVYQLSGSSVQLGLAGFFQAFPFIVFGLFGGVLADTLNRKKLIAITHTLN
                     ILPGVALGLLTITGAVQVWQINALNIVASSLQVLGGPSRQAIIPGLVPQRLLLNAVTT
                     ATLMMQSTQLTAPVIAGFVIDLFGVQASYFTDAALLVPSVISALLIHASGHPQGQRRQ
                     ISWRSLIEGFEFLWHTRIILSLFLLDFFAVLFGFFRPILPIFADVVFKVGARGLGMLY
                     AAPAIGALVGSGLLLAAGDIKRKGALSIIVTLLFALSLGLLGLSQTFWMGLFAVGALG
                     FNDAISVAIRRTVVQILAPDDMRGRATSFLTVFAQTTNATGAVIAGAGAALLGAPHAA
                     LVGCLLCTLTIVGTCWAIPQLWHYRSD"
     gene            3082..4179
                     /locus_tag="VFY96_08745"
     CDS             3082..4179
                     /locus_tag="VFY96_08745"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5606589.1"
                     /translation="MYIDGDTHYWPVRFLDRVKHSGRGHIEITKGAGEMIRYGEKFPG
                     DAATYYRDGKKVHSFSETRWNLDMHQDVMAREGFDYQVVIADNRPLIYEIDHDLGVEM
                     ARAYNDTVAEDIGGRERFIGTAWVYLPDVPEAIRELRRAVKELGFKGVKLMGGHENCD
                     LDAEVLWPFYEEVCKLDVPILVHPGTRIYEAQDCHPWLVGSERYAGFKMLATALGFPL
                     TYMVSAARLIMSGAFDRFPTLRIAVFEGGVGWVPWLMRTLNHHTLGESSGTTSVNDFF
                     RKRDKLKKAPSEYFDQIYVAAIAWEDYLPQIVEMWPNHNIIIGSDFDHGDAISTWPNT
                     IAPIKAMKGISEEDKEKILGGNAARLFGMKG"
     gene            4680..5861
                     /locus_tag="VFY96_08750"
     CDS             4680..5861
                     /locus_tag="VFY96_08750"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEX5606590.1"
                     /translation="MTMPFRPRWYFFVALVSITFIGPLSIHLFIPAMPAVKEEFGVST
                     GIAQLTLSLGMLAMAFFTVAYGGLSDRYGRKRVLLGGLVLFTCGAASCMAAANLPMLL
                     AGRILQGAGAGCGVVLARAIARDVYGQDRVAQVIAYLTAAYVLGPMFAPPIGGQLTTL
                     FGWRALFVLASAVGLLVIVAVAFAVPETRARNIGVSRSVFAGYKSLLRRPRFVGFMLQ
                     PGMMSAAFYTQATAASFLAAEHLGADAAKIGVWFFAFPMGFMAGSFISGRIGAKRSIE
                     FMTILGGVIGVVNGTLLAGWLYFGGVSMAALYIPGFFVSLAQGLSMPYAQAGAMAVDT
                     ELAGSASGAVVFSQLFWPAALQELTGLLADGTWVPMAAVQFAAVIVALLAGWMAARAE
                     I"
     gene            complement(5904..6797)
                     /locus_tag="VFY96_08755"
     CDS             complement(5904..6797)
                     /locus_tag="VFY96_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007789783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5606591.1"
                     /translation="MQRENFNDILAFLAVAQERSFTRAAAKLGVSQSALSHTISGLEA
                     RLGLRLLTRTTRSVSPTEVGERLLLTVGHRFEEIEAELQALSELREKPAGTIRITATD
                     YAADTILWPKLAKFLREYPDIKVEIIIDNGLTDIVAQRYDAGVRSGEQVAKDMIAVRI
                     GPDMRMAVVGAPSYFAQRSQPKKPQDLNDHSCINLRLPTHGGLYAWEFEKGGRELKVR
                     VEGQLVFNGTFQMLNAALAGFGLAYVPEDLAQPHVAKGRLKQVLEDWCPLFSGYHLYY
                     PSRRQSAAAFALLVDALRYRS"
     gene            6918..7313
                     /locus_tag="VFY96_08760"
     CDS             6918..7313
                     /locus_tag="VFY96_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5606592.1"
                     /translation="MNIKRSGSQPSGKGPAEYFTGTVRIDPLFEAPDPARVRGARVTF
                     EPGARSAWHTHPLGQTLIVTAGSGWVQSEGGPKAEIRPGDVVWCPPKEKHWHGATTSA
                     AMTHIAIQEALDGKVVEWMEKVSDEEYQA"
     gene            7405..7905
                     /locus_tag="VFY96_08765"
                     /pseudo
     CDS             7405..7905
                     /locus_tag="VFY96_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540720.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
BASE COUNT         1680 a         2229 c         2378 g         1618 t
ORIGIN      
        1 gtccaagaag ggctggtgct ggtcaacgcg atgcatatta ccgcgtcggt tttcatcaac
       61 gacgacgaat ccggtttgca tcatgactac accgtctggc tggagaagct cgcgccgcac
      121 gaaccgctca agcagtatcg acacaacgac accggcgagg acaatgccga cgcgcacatc
      181 aagcgccaag tcatgggacg tgaagtggtc gttgccatca ccggcggcaa gctcgatttc
      241 ggtccctggg aacagatctt ctacggcgag ttcgacggac ggcgccgcaa gcgcgtgctg
      301 gtaaaaatta tcggtgattg aggaagcttt ggcgagattc tatcaaaggc gcgatacgga
      361 agagcatggc cgatcggctc caatccagag ccgtcccact taccggcccc catgtcctca
      421 ttatccgcac tgcgtcgggt gcccgttgat cgatgtgccg tatccggagc aactggcgcg
      481 caagcgtgcg atcgttcttg aatcgtttgc tcgttatgcg tcgttggctg ctgtcgaggt
      541 gccgcgccta gcaccggcgc ctgagcgtct cggctatcgc acgcgggtca agctcgtcgc
      601 cagacgaaat cgcgaccaaa ttgctttggg actctacgtt cctagcagcc atcgcgtgat
      661 cgacatctcc tcttgtccgg tccacccgcg gcccgtcaat caagccgcgc agtatctcaa
      721 aaagaagatt ctcgagctcg gcatcatgcc ttacgacgag cgcgatgaca gcggcgatct
      781 gcgttacgtc gattttcgtt ttagcgctgc gcgccaggaa ttgagcgtga cactggtgac
      841 gcggcatagt tcgtttccgg ccggcgtccg gttagcgcac tcattgatga agcgctttcc
      901 cttcgtcacc ggcgtcaatc aaaacgtcaa cgaagcgcgc ggcaacgtga tctggggacc
      961 tgagttccgc acgctcgccg ggcgcgaaac tttgctggat cggatcggcg atctgaagct
     1021 ggttcttcct cccggcgttt tttcccaggc caatccttcg acggcgcgaa aactttacga
     1081 agcggtatgt aactttgccg atttaagtgg gcgcgaaacc gtccttgatc tctattgtgg
     1141 cgttgggccg atttcgctgt atctcggttc aacggcgcgc caagtttgga gcgtcgatga
     1201 tagtgagctc gcggtcaaca ccgcgaagca gaacgctcgc cgcaacgggc gcggcaattg
     1261 tcgatttctc gctggagatg tcgcttcgct ggcgacgaaa ctaaagaacg atctcggtga
     1321 tatagacttg gctgtggtca acccgccgcg caaaggcgtg cagccggtgg cgatgaaagc
     1381 gatcattgaa tgcggcgcgc ccaaactcat ttacgtttca tgcgagccaa cgacccttgc
     1441 gcgggatttg gatcggcttg tcaatggcgg gtaccgaatc gagcggttgg ctcttttcga
     1501 tatgtttccg cagacggagc aggtggagag tgtggcgctg ctcagcaagc gggaagtcta
     1561 gcctttcggg tctgacgtct cgtgtcgaaa atgcttcgac gagctcagca tgcgcggaat
     1621 ttctgggaga cttggacggt tttccgctca tcctgagact gtcgaagggt cgtaaaattt
     1681 tttcagccct tttaatccga tcgatagtgc cataactggg gaatcgccca gcaggtgccg
     1741 acgatggtca gggtgcacaa caggcaaccg accaatgccg catgcggcgc gcccagcagt
     1801 gccgcgccgg caccggcgat gactgcgccg gtggcattgg tggtttgagc gaaaacggtt
     1861 agaaagctcg tcgcccgccc ccgcatatcg tcgggcgcga gaatttgcac gaccgtgcgc
     1921 cggatcgcta cgctgatggc gtcgttgaag cccaaggcgc cgaccgcgaa aagtcccatc
     1981 cagaatgttt gcgacaaccc gagaagcccc aggctgagcg cgaaaagcaa cgtaacaatg
     2041 atcgaaagcg cgcctttgcg cttgatatct cccgccgcga gcaagaggcc cgaaccgacc
     2101 agtgcaccaa tggccggtgc cgcatagagc atgccgagac cgcgcgcccc gactttaaac
     2161 acgacatcgg caaaaatcgg caggatggga cgaaaaaaac caaacagcac ggcgaaaaaa
     2221 tcgagcagga acaaagagag aataattcgc gtgtgccaga gaaattcgaa gccttcgatc
     2281 aaactccgcc agctgatctg ccggcgttgg ccttgcgggt ggcccgaggc atggatcagc
     2341 agcgccgaga tcaccgatgg cacgagcagc gcggcatcgg taaagtagga agcttggacg
     2401 ccaaaaagat cgatgacgaa tccggcgatc accggcgcgg tcaattgagt gctttgcatc
     2461 atcaaggtcg ccgtcgtgac ggcgttcaga agcaagcgtt gcgggacgag acccggaata
     2521 atcgcttgcc gcgaaggtcc gccgagaact tgcagagacg aggccacgat gttgagcgcg
     2581 ttgatttgcc agacctggac ggcgccggta atcgtcaaca ggccgagcgc cacgccgggc
     2641 agaatgttta gcgtatgagt gatggcgatc agcttcttgc ggttcaacgt atcggcgaga
     2701 acaccaccga acaggccgaa gacgatgaac gggaaagcct gaaaaaatcc ggccaggccg
     2761 agctgcaccg aagagccgga cagttggtaa acctggtaga ggctcgcgac gttgcgcata
     2821 ttgttcgagg tgttggcgca gagaattgta atgaaaatga gccggtaatc gcggatcagg
     2881 agcgatgacc aggggcgaat cgaaattgcc gggtgcgact cgggaaggga agtctcttga
     2941 ggggatgaca ctcgggaatg aagtccttgc aaagatcgag cagtgctcca atctaatggt
     3001 tcgaaccatg cgcgtcaaat cgtattgacc tcaccgcccc tgcgctctag tatcccggaa
     3061 aatggactga gaggtttttc catgtacatc gacggtgaca ctcattattg gccggtacgg
     3121 tttctcgatc gggtaaaaca ttcgggccgc ggccatatag agatcacaaa aggcgccggc
     3181 gagatgattc gctatggcga aaaatttcct ggagatgcgg cgacctatta tcgcgatggc
     3241 aagaaggtcc attccttcag cgagacgcgc tggaatctcg acatgcatca ggacgtcatg
     3301 gcgcgcgagg ggttcgatta ccaagtcgtg atcgccgata accggccgct catctatgag
     3361 atcgaccacg atcttggcgt cgagatggcg cgcgcctata acgacacggt ggctgaagac
     3421 atcggtggca gagagcgctt catcggtacc gcgtgggttt atctccccga tgtgccggaa
     3481 gcgattcgtg agcttcgtcg cgccgtgaaa gagctcggat tcaagggcgt caagctgatg
     3541 ggcggccatg aaaactgcga cctcgatgcc gaggttttgt ggccgtttta cgaagaagta
     3601 tgcaagctcg acgtgccgat tctcgtccat ccgggcacgc gaatttatga ggcgcaggat
     3661 tgtcatccgt ggctggtcgg tagcgagcgc tacgctggct tcaagatgct tgccacggcg
     3721 ttgggatttc cgctgactta tatggtgagc gcggcgcggt tgatcatgag cggagctttt
     3781 gaccgcttcc cgactttacg catcgccgtt ttcgagggtg gcgtcggttg ggtgccatgg
     3841 ctgatgcgca cgctcaatca tcacaccctc ggcgaatcat cgggcacgac ctcggtgaac
     3901 gatttttttc gcaagcgcga caagttaaag aaagccccaa gtgaatactt cgatcaaatc
     3961 tacgttgcgg cgatcgcgtg ggaagattac ttaccacaga tcgtcgagat gtggcccaat
     4021 cacaacatta tcatcggcag cgacttcgac cacggcgacg caatttcgac ttggccgaac
     4081 accatcgctc cgatcaaagc gatgaaaggc atttctgagg aggacaagga aaaaatcctc
     4141 ggcggcaatg cggctcggct gtttgggatg aagggataaa cgcgggcgta aaactatcct
     4201 gcacgcccac gacgctttcg acaccgatac gtattttcaa ttttgcggca acagcaccgt
     4261 tgaaaccgag tttggcgttt atgagatgga gcccggcgaa gtgatgctgg tgccgggcgg
     4321 catcgcccat cggtccatgg acgcaatgac agcctgcgct acttctgccc gccaattttt
     4381 aatttgatct ccaaaccatc tctcggagag gtgtcaacag agttggcaga cgacaccgtg
     4441 ccaaccggcc agaactgcgc aggtagaact tacacgggcc ttcgaatttg gtacaaagcc
     4501 ggatcgacaa tcgggaccga catgtttaag tacaccatcg agtttcccca cgagccgtca
     4561 aatccaacgc cttcgaaagg tccacaacgc gtgagtgtca ccgttgtggt tcaatgagcg
     4621 cggatcaccg cgggaagggc gcgtgagacg ataacgcgcg cgacgctaat aattactcca
     4681 tgacgatgcc ctttcggccg cgctggtatt ttttcgtcgc gctcgtctcc attaccttca
     4741 tcggcccgct gtcgattcat ctattcatcc ccgcgatgcc cgcggtgaag gaagagtttg
     4801 gcgtctcgac cggtattgcg cagctcacgc tatcgctggg aatgctggcg atggctttct
     4861 tcacggtcgc ctatggcggc ttgtccgacc gctatggtcg caagcgcgtg cttctgggtg
     4921 ggctcgtgct gttcacttgc ggcgcagcca gctgcatggc cgccgccaat ctgccgatgc
     4981 tgctggcggg acgcatacta cagggagctg gggcaggctg cggcgtcgtg ctcgcgcgcg
     5041 ccattgcccg ggatgtgtac ggtcaggacc gcgttgccca agtaattgcg tatctgacag
     5101 ccgcgtacgt gctggggccg atgtttgcgc cgccgatcgg cggccaactg acgacgctgt
     5161 ttggttggcg cgcactgttc gtgttggcgt ccgcggtcgg gctgctggtg atcgtcgcgg
     5221 tggcgttcgc agtaccggaa acccgcgcgc ggaatatcgg cgtgtcgcgc agcgtattcg
     5281 ccggctacaa gtcgctgttg cggcggccgc gcttcgtcgg tttcatgctg cagcccggaa
     5341 tgatgtcggc ggcgttctac acccaggcga ccgcggcgtc attcctcgcc gcagaacatc
     5401 tcggcgccga tgccgccaag atcggcgtct ggttcttcgc cttcccaatg ggcttcatgg
     5461 ctggcagttt catctcgggc cgcatcggcg cgaagcgttc gatcgaattc atgaccatcc
     5521 tcggcggcgt gatcggagtc gtcaacggaa cacttctcgc gggttggctt tatttcggcg
     5581 gcgtttcaat ggcggcgctc tacatcccgg gattcttcgt gagtctcgct cagggcctgt
     5641 cgatgcctta cgctcaagcc ggtgcgatgg cggtggacac cgagcttgcc ggctcggcct
     5701 ccggcgcggt ggtgttctcg cagttgttct ggcctgctgc actgcaggag ctcacggggc
     5761 tgctcgcgga tggcacgtgg gtaccgatgg cggcggtgca gttcgccgca gtcattgtcg
     5821 cgctgctcgc cggctggatg gcggcacgag ctgagatcta gcgatcaaaa cacggagagt
     5881 gattcaattt aggttcgtcc gcattaagag cggtagcgca gcgcgtccac cagcagggcg
     5941 aaggccgccg cggattgtcg tcggcttggg taatagaggt gatagcccga aaacagaggg
     6001 caccaatctt caagcacctg tttgagacga cctttagcga catggggctg tgccaggtcc
     6061 tctggcacat aggccaaccc gaatccggca agcgccgcat tgagcatctg aaaagtgccg
     6121 ttgaagacga gctggccttc gacacgcacc ttcagttcgc ggccgccctt ttcgaactcc
     6181 caggcgtaca agccgccatg agtcggcagg cgcagattga tgcagctgtg gtcgttcaag
     6241 tcctgtggct tctttggttg cgaccgctgt gcgaaatagg acggagcgcc gaccaccgcc
     6301 atacgcatgt cgggtccgat acgcacggcg atcatgtctt tcgccacttg ctcaccggat
     6361 cgcacgcccg cgtcatagcg ttgcgcgaca atatccgtca ggccgttgtc gatgatgatc
     6421 tcgaccttga tgtccggata ctcgcgcagg aatttcgcca gtttcggcca gaggatcgtg
     6481 tccgccgcat agtccgtagc cgtgatccga atcgtaccgg cgggcttctc gcgaagctcg
     6541 cttaaagctt gcagctcggc ctcgatctcc tcaaagcggt ggcccacagt cagcagcagc
     6601 cgttcaccca cctcggtcgg ggagacgctg cgcgtggtgc gagtcagcag ccgcagcccg
     6661 agtcgcgcct cgagtccgct aatcgtgtgg ctcagcgccg attgagaaac tccgagcttc
     6721 gccgccgccc tggtgaagct tcgctcctgc gcgacggcga ggaaagcgag aatgtcgtta
     6781 aagttttcgc gctgcattta tgcagaaatc tcataggtcc atgcgaaatc tactctctaa
     6841 tcgcatggcg ccgcaacggc tatattttcc tcatcagata agtcatggct gcgattaaaa
     6901 ggagaaagga atcagcgatg aacatcaaaa gaagcggctc acagccttcc ggcaagggac
     6961 cggcggagta ttttaccggc actgtacgca tcgatcccct atttgaggcg ccggatcccg
     7021 cacgcgtgcg cggcgccagg gtcaccttcg agccgggagc tcgaagcgcc tggcacacgc
     7081 atccgctcgg ccagactctg atcgtcaccg ccgggtccgg atgggtccaa agcgaaggcg
     7141 gtccaaaagc agaaatacga ccgggcgacg tggtctggtg tccgccgaag gaaaaacatt
     7201 ggcatggcgc gacgacgagc gccgccatga cgcacatcgc catccaggaa gcgctcgacg
     7261 gcaaggtcgt cgagtggatg gaaaaggtca gcgacgaaga ataccaggcc tgaatgccaa
     7321 gtcgttgctt ccgaacttca acgcagccgg ggagtttaaa atgcaaaaac gcaacttgga
     7381 aaaagcaatt tggaagtctc tgccaaggtc aagcacttcg gcctttcgga accgggagta
     7441 caaacaattc gccgcgcaca cgcagtccag tcggtcacag ccgtccagag cgaatactct
     7501 ctgtggtgga gaccgctcga agaggaattg ctgccaaccc tcgaggaact cggaatcgga
     7561 tttgttccgt tcagtcctct gggcaaaggc tatcttaccg gaaagatcga cgagaatacc
     7621 aagtttgata gctccgattt ccgcaatatc gttcctcggt ttacgccgga tgcgcgcaaa
     7681 gcgaatctgg gtttagttga tctgcttcgc aagatcggag aacggaagaa ggcgacacct
     7741 gctcaaatcg cacttgcctg gctgcttgcg cggaagccct gggtcgtgcc cattccgggc
     7801 accacgaaac tgaaccgtct ggaggagaac atccgcgcgg ttgcagtgga actgacgtcc
     7861 caggatttgc atgagatcga cgccgccact gcaaagatca ccatc
//
LOCUS       DASULZ010000431         3864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_54126, whole genome shotgun sequence.
ACCESSION   DASULZ010000431 DASULZ010000000
VERSION     DASULZ010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54126"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VFY96_08770"
     CDS             <1..365
                     /locus_tag="VFY96_08770"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX5606593.1"
                     /translation="YNLQKNFDGAVSMGPCLVVGEADPQDIDAETRINGVVRQSYNTK
                     EMIWSFGEVLEYLSRDFTFVPGDVIAGGTSAGTAADKSRRGPDGKRPLDLFLKVGDTV
                     AVSSSRIGILANKIVASE"
     gene            459..1622
                     /locus_tag="VFY96_08775"
     CDS             459..1622
                     /locus_tag="VFY96_08775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5606594.1"
                     /translation="MDFNLPEEIQILRDTVRKFVDKELIPLERVYRHDSEGPMPEHLL
                     KPLQETAKAMGLWMLDVPAEYGGAGLGLLPRCIIHEEIARAASLPFRSLELFGPEVRP
                     VLFHCNDEQKKRFLEPVLRGELKFCFAQTEPDAGSDPANMRTRALQDGDHFVINGTKR
                     FITAAGRADYAQLMAVTDTEKRARGGITCFAVDLKGAGVLLERQWPAMMGEAPWQIVF
                     DNVRVPAANVIGAVGGGFSLAQKWIGEGRIRGHGARPVGIARRALDMMIEYSKVRVTF
                     GRPLADRQAIQFMIADSAMELHAVRNMVYECAWRVDRGEDVRDLSYMVKIMATEMASR
                     VVDRAIQVHGGLGLMKELPLEWWYRQIRSTRITEGANEVLRWRLGQNIIRAHR"
     gene            complement(1739..2506)
                     /locus_tag="VFY96_08780"
     CDS             complement(1739..2506)
                     /locus_tag="VFY96_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006718301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5606595.1"
                     /translation="MELNGRVALVTGGGGGIGGAVVRCLARAGVSGVIINYRRSAKEA
                     EALALEIERAGVRAMALQADVQNDDQVKSMIARIGAEFGRLDILVNNAGVTHWVKVSD
                     LEGLTDAIWDEILDVNVKGAFRCARAAAKLLEANSGMIVNVSSISGVLSPSTMSSLAY
                     GTAKAALIHLTKGLAVALAPKVRVNCVAPAFTDTPWMSGHYGEKYEQVIEQAATGYPL
                     QRIAMPDEVAGAIIGLITGGDFVTGQTLIVDGGLSLS"
     gene            complement(2787..>3864)
                     /locus_tag="VFY96_08785"
     CDS             complement(2787..>3864)
                     /locus_tag="VFY96_08785"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX5606596.1"
                     /translation="SETAVRAEIRKLPNGEWTNETWSDGFEEPIVVRCTVKIADEDIF
                     IDFTGSSPQSTRGINVVLNYTHAYASFAIKAAVCPDVPHNDGSFRPVHVSAPPGSILN
                     ALDPAPVASRQVIGHFIPSAIFAALSGAMPDRLMAPGADPIWLSVWRGQNPPFTLTVF
                     QVGGTGARPAKDGLSAVGFPSGVAGVPAEVIESLSPVVMKRRELRPDSGGPGEWRGGL
                     GQLTEFTRRGAGRWSVSSIADRTVYAAPGLHGGESGATGEVTLDDGTRLNAKALKDLK
                     ERDVVHVNLPGGGGYGDPFARDPEKVRWDVIEGYVTPDEAEKRYGVAVRYSGNADDLV
                     KLAKLWTVDETRTAALRSGRGTIE"
BASE COUNT          791 a         1219 c         1053 g          801 t
ORIGIN      
        1 gttataattt gcagaaaaat ttcgacggcg ccgtgtcgat gggtccgtgt ctcgttgtcg
       61 gcgaggccga tcctcaagac attgacgccg aaacacgcat caatggcgtg gtgcggcaaa
      121 gctacaacac caaggaaatg atctggtcat tcggtgaggt gctcgagtat ctatctcgag
      181 acttcacctt tgtccctggt gacgtcattg cgggtggcac gtcggccgga accgccgccg
      241 acaaatcgcg ccgtggaccc gatggcaagc gcccgctgga tctgtttttg aaagtcggtg
      301 ataccgtggc agtctcatca tctagaatcg gcatactggc aaataaaatt gttgcaagtg
      361 aatagcgggt ctgtaatcac cgcatcaaca taggggcgtc acattggacg ccctacatgc
      421 gcgatcgcgg ccggttcaac cgtcagtaag aaaacgtcat ggattttaat ctccccgaag
      481 aaattcaaat cctgcgcgac acggtgcgca agtttgtcga taaagagctg attccgctcg
      541 aacgtgtgta ccgccacgac agcgaaggac cgatgcccga gcatctgctg aagccgctgc
      601 aggagactgc caaggcgatg ggactttgga tgctcgatgt tcccgccgag tacggcggcg
      661 ccgggctcgg tttgctgccg cgctgtatca ttcacgaaga gatcgcacgg gctgcttccc
      721 tcccatttcg tagcctcgag ctctttggtc ccgaggttcg tccggtgctt ttccattgca
      781 atgacgagca gaaaaaaaga tttctcgagc cggtcttgcg cggcgagttg aaattttgtt
      841 tcgcccagac cgagcccgat gccggaagcg atccagccaa tatgcgaacc cgcgcgctgc
      901 aggatggcga tcatttcgtc attaacggca ccaagcgttt catcacggcg gcgggtcgcg
      961 ccgattacgc gcagctgatg gccgtcaccg acacggaaaa acgcgcgcgc ggcggcatta
     1021 cctgtttcgc ggtcgatctg aagggcgccg gagtgctact cgaacggcaa tggccggcca
     1081 tgatgggcga agcgccgtgg cagattgttt tcgataatgt ccgagtgccg gcggcgaacg
     1141 tgatcggcgc cgtcggagga ggtttttcgc tggcacagaa atggatcggc gaaggacgta
     1201 tccgcggcca cggcgcgcgc ccggttggta ttgcccgccg cgctctcgat atgatgatcg
     1261 agtattccaa agtccgcgtc acctttggtc gaccgctggc ggaccgacaa gccatccagt
     1321 tcatgatcgc ggactcagcg atggagctcc acgccgtcag aaacatggtc tacgagtgtg
     1381 cctggcgcgt cgatcgcggc gaagacgtgc gcgacctctc atatatggta aaaatcatgg
     1441 ctaccgagat ggccagtcgc gtcgtcgatc gtgcgattca ggttcacggc ggattggggc
     1501 tgatgaagga attgccgctt gaatggtggt accgtcagat ccgcagcacg cgcattaccg
     1561 aaggcgccaa tgaagtcctg cgctggcgcc tcggccagaa catcatccgc gcccatcgct
     1621 aagatagttc cagtcgacat ttagcaagcc atctgtcgca attgacgctc cctgtatttt
     1681 tccagtgcac tctcgagtgg cgtaaagggc cggcgtctcc tctaacaaaa ggtcctcttc
     1741 agctcaagct caatccgcca tccacgatca acgtctgtcc ggtcacgaaa tcgccgccgg
     1801 tgataagacc aataattgcg ccggcgactt catccggcat ggcaatacgc tgaagtggat
     1861 atccggtcgc agcctgctcg atcacctgct catatttttc cccataatgg ccgctcatcc
     1921 agggcgtgtc ggtaaaggcc ggcgccacac agttcacgcg cactttcggc gccagcgcca
     1981 cggcaagtcc cttggtcaga tgaatcagcg ccgcctttgc cgtgccgtac gcgagcgatg
     2041 acatcgtcga gggcgaaagc accccggaaa tcgatgaaac gttgacgatc atcccgctat
     2101 tggcttcaag aagtttagcc gccgcccgcg cgcagcgaaa cgcgcctttg acattcacgt
     2161 cgagaatctc gtcccaaatc gcgtccgtaa gcccttcgag atcggaaact ttgacccagt
     2221 gggtcactcc cgcattgttc acgagaatat cgagccggcc aaactcggcg ccgattctcg
     2281 cgatcatcga tttcacttga tcatcgttct gcacatcggc ttgaagcgcc atcgctctaa
     2341 cgccggcgcg ctcgatctcc agcgccagcg cttcggcctc tttcgcagat ctgcggtaat
     2401 taataatgac cccgctcaca ccggctctag ccaggcaccg caccacggca ccgccgatcc
     2461 caccgccgcc gccggtcacc aatgccaccc gtccgttcag ttccatgttt tcaccagatt
     2521 tgtactgctc acgggaaagc tcacgatgcg aaactcgttg ttcgtcactc gataggccat
     2581 gatttaccct actctatcac ttcgccgatc aacccgactg cccgagtagc gcaaagcgca
     2641 tctcgaggga ttcgctctcc gccattttgt tacgtccctc gctacacgac cgtgacggtc
     2701 gctacgcggg gacgggcgtc tcgagcgtcg tatctcgaac cgctctagga ctcaggtaaa
     2761 ctccctgaga ttttgcttgt gattttttat tcgattgtgc ctcttccact ccggagcgcc
     2821 gccgttctcg tctcatcaac ggtccagagc ttggccaact tcaccaaatc atcggcattt
     2881 cctgaatacc gcaccgccac accgtatctt ttttccgcct catccggcgt tacatagccc
     2941 tcgattacat cccagcgcac cttctccggg tcacgagcga acggatctcc gtacccgccg
     3001 ccgccgggaa gatttacatg aaccacatcg cgctctttca agtcctttaa cgccttggca
     3061 ttcaatcgcg tcccatcgtc cagcgtgact tcacccgtgg cgccggactc gccgccatgc
     3121 agaccaggcg ccgcatatac cgtgcgatcg gcgatagagc tgacgctcca acgtcccgca
     3181 ccgcgccggg taaactccgt caactgcccc aagccaccac gccactctcc cggcccgccg
     3241 gaatccggcc gtaattcgcg tcgtttcatc accaccggcg atagactctc gatcacctcc
     3301 gcgggcacgc cggcaacgcc gctcggaaaa cccacggcgc tcaaaccgtc cttcgccggt
     3361 cgcgcacccg tgccgccgac ctgaaaaacg gtcaacgtaa atggcggatt ttgaccgcgc
     3421 caaacgctca gccagatcgg gtcggcgccc ggcgccatca agcggtccgg catcgcgccc
     3481 gacagagccg caaagattgc gctcggaatg aagtgaccga tcacctggcg gctcgccacc
     3541 ggcgccggat cgagggcatt gagaatgctc ccgggcggcg cgctcacatg gacggggcga
     3601 aagctgccat cattgtgggg cacgtcggga cacactgcgg ctttaatcgc gaagctcgcg
     3661 taggcgtgcg tgtagttaag aacgacattg atgccgcgcg tgctctgcgg cgaagaaccg
     3721 gtgaaatcga taaaaatgtc ctcgtcggcg attttgaccg tacagcgcac gacgatcggc
     3781 tcttcgaagc catcgctcca ggtttcgttt gtccactcgc cgttcgggag cttgcgaatt
     3841 tccgcccgca ccgccgtctc ggag
//
LOCUS       DASULZ010000432         5077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1169888, whole genome shotgun sequence.
ACCESSION   DASULZ010000432 DASULZ010000000
VERSION     DASULZ010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169888"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="VFY96_08790"
     CDS             <1..962
                     /locus_tag="VFY96_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606597.1"
                     /translation="RLVRSLSSVTMPIVETDPWRTQYFMAVACPEHVVIPTDDELAYQ
                     QFPRHRWIYNKLLICDTQGLDNAPHDIMPRQFPVFSKPIYNMRGMGSGSKIVESPEQF
                     RAAVQPGHMWMPLLVGEHVSTDAAVIDGEPVWWRHTIGKTLDQGMFDYWVVLSESKPE
                     IESYCGEWLRRHFRGYTGCVNFETIGSKIIEAHLRFADQWPDLYGSGWIDAVVELYAT
                     GHWRYRDEDRRTGYSIVLFAPHGRRYKQLDPSAVNEILNWEGISSIQMTFHEDKAPEL
                     HSMPPGGFRVAIVNCWNPEIGFKARERLAYYFHSTPCSAKVAA"
     gene            1144..3174
                     /locus_tag="VFY96_08795"
     CDS             1144..3174
                     /locus_tag="VFY96_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004998608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX5606598.1"
                     /translation="MKNIHRFPHAVRVIENSWIPMSDGCRLAARIWLPTDAETAPVPG
                     ILEYIPYRKRDFTRGRDEPMHHYFAGYGYAAVRVDLRGSGDSDGLLLDEYLQQEQDDA
                     IEVIRWIAQQPWCSGVVGMMGKSWGGFNSLQVAARCPPELKAIITVCSTDDRYLDDVH
                     YMGGCLLNENLTWGSVLMSHNAYPPDPEIVGEQWRTMWRDRLEHAVFFPEVWLSHPHR
                     DDYWKHGSVCEDYSAITCPVYAIGGWSDAYSNAIPRLLAGLKVPRKGLIGPWAHLYPH
                     DGIPGPAIGFLQEALRWWDQWLKGKDTGVMAEPMLRVWMQESVRPRPFHKQRPGRWVA
                     EKQWPSPRITLERYWLDQDRLSKAPTEDIRLDICSPQTTGLRAGEWCGFGAPGEAPPD
                     QRADDGCSLTFDSEILQERLEILGAPVAILELATNRPVPFITVRLNDVAPEGTSARVT
                     YGLLNLTHRDGHDTPEPLEPGKRYIVTVTMNAIAHAFPAGHKVRLALSTCYWPIAWPA
                     PRPVTLSVFTANSFVDLPMRAPDPSDSALRSFESPERAASEVTELRPAAVKRIVERDR
                     ATDETVYTVSSAPGDLDSPELLYIKAIDLEVGYTLLKRFRIAEDDPATAQAEVIHKTW
                     FCRGGWKPRVETHTCFSSNSADFILQAEVRAYENGEQFFRRQWFARVKRDLV"
     gene            complement(4583..>5077)
                     /locus_tag="VFY96_08800"
     CDS             complement(4583..>5077)
                     /locus_tag="VFY96_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HEX5606599.1"
                     /translation="VGVIGSSYGGYLAAILTNMRPIRWLALRAPALYEDQGWEAPKVE
                     LNEKMDLTAYRRRLVSPDENRALGACSAFRGDVLIVESENDQVIPHTVIANYVAACTQ
                     ARSVTSRVITGAQHGLSEQQWQGAYASILTKWLAEMMGSNDQVQPKNPVAGSLSNKVT
                     TPLE"
BASE COUNT         1128 a         1417 c         1353 g         1179 t
ORIGIN      
        1 taaggctcgt tcgctctctc agtagtgtaa ccatgccgat tgtagaaaca gatccatggc
       61 gtactcagta cttcatggca gttgcttgcc ccgagcatgt ggtcattcca accgatgacg
      121 aactggctta tcagcagttc ccgcggcatc gctggatcta caacaagctt ctcatatgtg
      181 acacgcaggg gctggacaac gcgccgcatg acatcatgcc tcggcaattt cccgttttca
      241 gcaagccaat ttacaatatg cgcggcatgg gctcgggtag caaaatcgtt gaatcgcccg
      301 agcagttccg cgccgcggta cagccgggtc atatgtggat gccccttctc gtcggcgagc
      361 atgttagtac ggatgctgca gtgatcgatg gcgagccggt ttggtggcgg cataccatcg
      421 gcaaaactct cgaccaggga atgttcgatt actgggtggt ccttagcgaa tcaaaacccg
      481 aaattgaaag ttattgcggc gaatggctgc gtcgccattt cagaggttat acggggtgcg
      541 tgaacttcga aacgatcggc agcaaaatta ttgaagcgca cctgcgcttt gccgaccagt
      601 ggccggacct ctacggttcg ggttggattg acgcggtagt cgagctttat gcgacgggcc
      661 actggcgtta tcgcgatgag gatcgtcgga ccggttacag tatcgtgctc ttcgcaccgc
      721 acggtcgccg ctacaaacaa ctcgatcctt cagcagtcaa tgagatcctc aattgggagg
      781 gaatttctag cattcagatg acctttcacg aagacaaagc tccggagctg cactcgatgc
      841 cgcctggcgg ctttagggtt gccatcgtca attgctggaa tcccgaaatc ggcttcaaag
      901 cccgcgaacg actcgcctat tacttccact cgaccccatg cagcgccaaa gtcgccgcat
      961 agaatttcgc aaaaaatagc aaagctcgat caaccagtgc gcacgagttt gccttacggc
     1021 tggctggtca tcagctggcg ttctgcacgg atttgttcaa caaatgctct gccccccacc
     1081 gtcgcgtatg gaagggtcgc gtgaatcatc cggacgggta taatgcttcg gcaaagagtc
     1141 cgaatgaaaa acatccatcg attcccccac gctgttcgcg taattgaaaa ttcttggatt
     1201 ccgatgtcgg acggttgccg tctcgcggcg cgcatctggc tgccgacgga tgccgagacc
     1261 gctccggtgc cgggcatcct ggaatatatt ccgtaccgca agcgagactt cacgcgcgga
     1321 cgcgacgagc ccatgcacca ttacttcgcc ggttatggat atgccgcggt gcgagttgac
     1381 ctccggggtt caggcgattc agacggcctg ttgttagatg aatatttgca acaggaacaa
     1441 gacgatgcca tagaagtgat ccgctggatt gcgcaacaac cctggtgtag cggcgtcgtc
     1501 ggcatgatgg gaaagtcatg gggcggattc aattcattgc aagtcgctgc ccgttgcccg
     1561 cctgaactta aggcgatcat cacggtttgt tccacagacg accgctatct cgatgacgtg
     1621 cattacatgg gtggttgcct gctgaacgaa aatctaacct gggggtcggt gctcatgagc
     1681 cacaatgcct atccgccgga tcctgagatc gtcggcgagc aatggcgcac catgtggcgc
     1741 gatcgtctcg aacacgcggt attctttccc gaggtctggc tcagccatcc gcaccgagat
     1801 gattactgga agcatggctc cgtctgcgag gactacagcg cgatcacctg cccggtctac
     1861 gccatcggcg gctggtccga tgcttacagc aacgcaattc cgcggctgct ggctgggctc
     1921 aaagtcccgc gcaaaggtct catcgggcca tgggcgcatc tctaccccca tgacgggatt
     1981 ccaggtccag ctataggttt tctacaggag gcactgcgct ggtgggatca atggttgaaa
     2041 ggcaaagata cgggagtcat ggcggaaccg atgctgcgcg tttggatgca ggaaagcgtt
     2101 cggccacgcc catttcataa acagcgcccc ggccgctggg tagcagaaaa acagtggcct
     2161 tcgccccgga ttacactcga acgatactgg cttgatcagg atcgattgag caaagcaccg
     2221 acggaagaca tacgacttga catctgctcg ccgcaaacca ccgggctccg cgccggcgaa
     2281 tggtgtggat ttggtgcgcc gggagaagcg ccgcccgatc agcgcgctga cgacggttgt
     2341 tctttaactt tcgactctga aatccttcag gagcgcctcg aaattctcgg tgcccccgtg
     2401 gcaattttgg agcttgccac gaaccggcca gtgcctttta ttaccgttcg cttgaacgac
     2461 gtcgcacccg aggggacatc ggcaagggtg acttatggac tgctaaatct tactcaccgc
     2521 gatggtcacg acacaccaga acctctcgag ccgggtaagc gctacatcgt aaccgtcaca
     2581 atgaacgcta tcgctcacgc atttcctgct ggccataaag tgcgcttggc tctctcgacc
     2641 tgctattggc cgatcgcttg gccagcgccg cggcctgtaa ccctgagcgt tttcacggcc
     2701 aatagttttg tcgatcttcc catgcgagca ccggacccga gcgacagtgc ccttagaagt
     2761 ttcgaatctc ctgaacgtgc cgccagtgaa gtaactgagt tacggccggc cgccgtgaag
     2821 cggatcgttg agcgtgaccg tgcaacagat gagacggtct ataccgtctc cagcgcacct
     2881 ggtgacctcg acagtcccga attattatat atcaaggcga tcgatctcga ggtcggctat
     2941 actttactca agcgttttcg catcgcggag gacgacccgg ccacggctca ggctgaagtg
     3001 attcacaaga cgtggttttg tcgcggcggt tggaagccgc gggtcgagac gcatacctgc
     3061 ttctcctcga attccgcgga tttcatcctg caagcggaag ttagagcata tgaaaatggc
     3121 gagcagttct tcaggcgcca gtggtttgct cgcgttaagc gggatctcgt ctgaacgggt
     3181 tgcggccccc ctctttttct tgttacggcg tgcaggatac ttgacgtaat agaaaagaac
     3241 gatctcaagt ccttactaac cggaaaagtg gcggcatctt cagcgcgtga gcgcaatgcg
     3301 atttggcagt agggaatgac aatgtgtcaa agccgtcgtg taatttttgc caatcacact
     3361 gccccaaaac cctaggcgtt tcagatcctc gttagaactg tggcggaaat ggttgtcgtt
     3421 gtaatacttt gccgttgtat tccccttcag gttacgtgcc gcaatcttgg catttcttat
     3481 gctttcctcg attctagcca acttagaggc atggataatt tgtctttact cagaggaatc
     3541 acggtgccag ctaaccctgc tgcagaagca ttacgcattg tcggtcgcga tctggatcta
     3601 cgcgggatca ggacgtttat cattcggtgc gatcgagatc tttttgtcgt taccggtggc
     3661 tatcaacctc ctccagctgt tatgccggtg acagtgcact atacctccag cgatatcgac
     3721 cgccggctca tcgaggcaaa gaacaacgat tgcctatcag cagcaaggga tttttcgagc
     3781 ctggcggaga ctttgtcagc aatcggaagt tacgtaacca aaagacaggg tcgcttaatc
     3841 agcgtttcta acaccgcctc gacgccaaca attccaatca tcatagtcaa gtatgcgtca
     3901 cttgaatccg accctgttgt ggagattttt aaaggctccg caatttatga gttgtgtgtt
     3961 agcgcctaca aggcaagctt acctcgaacc gatctacact atactcgatt cagttgcttg
     4021 agcgatcagc gacattaacg gagctactgc tttatgctcg cgaaattgag accgatctcg
     4081 cgaacccttg cgacgtggac atcatccagt agggctgcaa cgaatttcca tcgcccttta
     4141 gcatatcggc cctacaaacg aatgtggcgt aagaactgga acgaaagcga gtgggcatat
     4201 gccagccgct gtgcccgggc actatttaac ttgtatggga gacgatctgg acggctattc
     4261 ttcattaacc gtcgcccatg gcgctggcag atcccaacca agtcaatccc atgagctaat
     4321 gcgctgattt ggccatggac aggcgagcgc agcaacccta cctcacagaa tatttctcgt
     4381 ttcctgagca tccgtctcac gattaggaac agatttcatg atattcgagc aatactcacg
     4441 ctttgtttgc tgagcagaac gcgcctttcc actccgttgg gcacaagtct tagagcctgc
     4501 cacactaaaa tattcgacga cttggaatcc tcacccgaga actttccaag tgcctaattg
     4561 gcacggatca ttactgtctt gcttactcga ggggcgtcgt gaccttgttg gacagagatc
     4621 cggccacagg attttttggc tggacctggt cattgcttcc catcatctcg gcaagccact
     4681 tggtcaggat cgaggcatac gctccttgcc actgctgctc ggaaagccca tgttgcgcac
     4741 ccgtaatgac gcgagaagtc accgacctcg cctgcgtgca agcagcgacg taattggcga
     4801 tcacggtgtg tggaatcacc tgatcatttt ccgactcgac gatgagaacg tcgccgcgaa
     4861 acgccgagca cgcacccagt gctcggttct catctggact caccagccgg cggcgatagg
     4921 cggttaggtc cattttctca ttaagctcca ccttcggcgc ttcccagcct tgatcttcgt
     4981 acaaagcagg cgccctcagc gcgagccagc gtattggccg catattcgtg aggatcgcgg
     5041 ccaaatatcc gccgtagctg ctcccaatca cgccgac
//
LOCUS       DASULZ010000433         3309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_763622, whole genome shotgun sequence.
ACCESSION   DASULZ010000433 DASULZ010000000
VERSION     DASULZ010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763622"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..527
                     /locus_tag="VFY96_08805"
     CDS             42..527
                     /locus_tag="VFY96_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5606600.1"
                     /translation="MAITKDQERALELIEQAMKHQMAREFDEAIQLYKKSIAVYPTAD
                     AHTYLAWAYSFLGRLNEAIAQCEIAIQLDPEFGNPYNDIGVYLMQQQRFDDAIPWLER
                     AKSAKRYEPRHFPHINLGRIYLTKGMLQKALEEFNGALKINPDDQELAQLVEELQTKL
                     Q"
     gene            complement(556..1857)
                     /locus_tag="VFY96_08810"
     CDS             complement(556..1857)
                     /locus_tag="VFY96_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016875338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HEX5606601.1"
                     /translation="MKPPHIKTALPGPKAKSIVDRDHRVTAPAYGRVYPLVVKRASGM
                     TVEDVDGNLFLDFMAGIAVASTGHSHPRVVQAIEEQARKFLHICGSDYYYEPMVALAE
                     KLGRLAPGSGAKKVFFTNSGTETIEAAIKLARYHTKRQHIIAFHGAFHGRTLGSLSLT
                     ASRASHRAHFGPLIPGVHHVPYPKYDSRAIESLQIIEKVLFRHEVRPEEVAAVFVEPI
                     QGEGGYLVPPAEYLPMLQDLCRKHGILLVVDEIQSGFGRTGKMFASEHWGLEPDIVCA
                     AKGIASGMPLGAMIARAEISTWPPSSHGSTFGGNPVACAAALATIELLEEGLVENAAE
                     MGALLKERLTALGRRHSTIADVRGLGLMVGVEFCRPNDGNAPDRQLRERIMQKCFEKG
                     LLLLGCGESTLRFCPPLIVTAAEVETAVRLFDEAIPEAIGA"
     gene            complement(1940..>3309)
                     /locus_tag="VFY96_08815"
     CDS             complement(1940..>3309)
                     /locus_tag="VFY96_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016867357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5606602.1"
                     /translation="AEDVDAAVQAAKSAFATWRLVPAPKRGEILYRVGELLRNHKEEI
                     ARGMTREMGKVLKETRGDVQEGIDTAFYVAGEGRRLFGETTPAELPDKFAMSVRMPVG
                     VCALITPWNFPVAIPTWKMFPALLCGNTVVLKPAEDTPGSAARLVEILNEAGVPPGVV
                     NLVHGRGEEVGAALVRHPDVQLVSFTGSAAVGREIASVGGQLLKRVSLELGGKNAQIV
                     MEDADLELAVEGALWGAFGTTGQRCTATSRLIVHRDVAKKMTDALVARSRQLKIGDGL
                     DESTDMGPLINQAAREKILNYISIGKQEGARLLIGGGIYDSGACAKGYFFQPTIFDRV
                     TSKMRIAQEEIFGPVLSIIEIGSFEEAISVLNDSRYGLSSSIYTRDVSRACRAIRDVQ
                     AGITYINGPTIGAEVHLPFGGVKDTGNGHREAGTTVYDIFSEWKSVYIDYSGKLQKAQ
                     IDNAE"
BASE COUNT          714 a          998 c          892 g          705 t
ORIGIN      
        1 gtctgagtag atcaacggat cggttgcaaa tctgagcgaa aatggcgatc actaaggatc
       61 aagaacgcgc cctggagctc attgagcaag cgatgaaaca ccagatggcg cgtgaattcg
      121 acgaggccat ccaactctat aagaaatcga tcgcggttta tcccaccgcc gatgcacaca
      181 cctatttggc gtgggcgtat agctttctcg gaaggctcaa cgaggccatt gcccaatgcg
      241 aaatcgccat tcagctcgat cccgaatttg gcaatcccta taatgacatt ggcgtttatc
      301 tgatgcagca acaacgcttc gatgacgcca ttccatggct cgagcgcgcc aaatcggcca
      361 aacgatacga gccgcgtcat tttccgcaca tcaacttggg caggatctat ctgaccaaag
      421 ggatgctgca aaaagcgctt gaagaattta acggcgcgct aaagatcaac ccggatgatc
      481 aggagctcgc gcaactggtc gaagagctgc agacaaagct gcagtaattt tttgggcgtg
      541 ccccgccgaa aatactcaag ctccgattgc ctcgggaatc gcttcatcga atagacggac
      601 ggcggtttcc acttccgccg cggtaactat taaaggcggg caaaacctca gcgtgctctc
      661 gccgcagccg agcagcaata atcctttttc aaagcatttt tgcatgatgc gctcgcgcaa
      721 ctggcggtct ggcgcattgc cgtcatttgg gcggcagaac tcgacgccga ccatgagtcc
      781 caagccgcgc acgtcggcta tcgtgctgtg acgcctgccg agcgccgtta agcgctcttt
      841 cagtagcgca cccatttcag ccgcgttctc gaccaaacct tcctctaata gctcgatggt
      901 cgctaacgcc gccgcacacg cgaccggatt accgccgaac gtactgccat ggctgctcgg
      961 cggccaggtg ctgatttcag cgcgcgcgat catcgcgccc agtggcatgc cgctggcgat
     1021 acccttggcc gcgcaaacaa tgtcgggctc gaggccccaa tgttcgctcg cgaacatttt
     1081 accggtgcgg ccgaagcccg attgaatctc atcaacgacg agaagaattc catgttttcg
     1141 gcagaggtcc tgaagcatcg gcaaatattc cgcgggtggc acgaggtatc ctccctcgcc
     1201 ctggatgggc tcgacgaaca ccgcggcgac ttcctcggga cggacttcat gccggaagag
     1261 aactttttcg ataatctgca gagattcgat ggcgcgtgaa tcgtacttcg gataagggac
     1321 atgatgcacg ccagggatca acggtccgaa atgcgcacga tgcgatgccc ggctcgccgt
     1381 cagcgataaa ctccccaggg tgcgtccgtg gaaggcgccg tgaaatgcaa tgatgtgctg
     1441 gcgcttggtg tgatagcgcg cgagcttgat cgcggcttcg atggtttcgg tgcctgaatt
     1501 ggtgaagaac accttcttcg cgccggaacc aggcgctagt cggccgagct tttccgccaa
     1561 agccaccatg ggttcgtagt aataatcgct gccgcagatg tgtagaaact tgcgcgcttg
     1621 ttcttcaatc gcttgaacga cacgcggatg cgaatggccg gtgctcgcta cagcgatccc
     1681 cgccatgaag tcgagaaaca gattaccatc gacgtcttcc acggtcatcc cggaggcgcg
     1741 cttcacgacc agcgggtaaa cccggccgta ggcgggcgcc gtcactcgat gatcacgatc
     1801 aacgatcgac ttcgccttcg gacccggcaa cgctgtcttg atgtggggtg gtttcatacg
     1861 gctccagttc cgcgtttcta gtctctcgct tttagttttt agttcttcca actgctaacg
     1921 agaatcttgg gactagaaac tattcggcgt tgtcgatctg cgctttttgc agtttgccgg
     1981 agtaatcaat atagaccgac ttccactcgc tgaagatgtc atagacggtt gtaccagctt
     2041 cgcgatggcc attgccggta tctttcacgc cgccgaaggg caaatgaacc tcggctccga
     2101 tggtcggccc attgatatag gtaatacctg cttgaacgtc gcgaatcgcg cgacaggcgc
     2161 ggctcacatc tcgcgtatag atcgagctcg acaggccgta gcgtgaatca ttcaatacgc
     2221 tgatcgcttc ttcaaaactg ccgatctcga tgattgaaag caccggcccg aagatttcct
     2281 cctgagcaat ccgcatcttt gatgtaactc gatcgaagat cgtcggctga aaaaaatagc
     2341 ctttggcgca agcgccagaa tcataaatgc cgccgccgat gagcaatcgt gcgccttcct
     2401 gcttgccgat gctgatgtaa ttgagaatct tctcgcgcgc agcctgattg atcaacggtc
     2461 ccatgtcggt gctctcatcg agaccatcgc cgatcttgag ttgccgcgag cgcgccacaa
     2521 gagcgtctgt cattttcttc gccacatctc gatgcacgat cagccgactc gtggcggtgc
     2581 agcgctgccc ggtggtgccg aaggcgcccc acagtgcccc ttcgaccgca agctccagat
     2641 cggcatcttc catcacgatc tgcgcgtttt tgccgccgag ctccaacgag acccgcttca
     2701 agagttgacc gccaaccgac gcgatctccc tcccaaccgc ggcggagccc gtaaatgaga
     2761 cgagctgaac atcgggatgg cggaccagcg ccgcgcccac ttcctcaccg cgcccgtgga
     2821 cgaggttcac cacaccgggt ggcacgcccg cctcattcag aatttcgacc agcctcgcag
     2881 cgctgcccgg cgtgtcttcg gcaggcttga gcaccacggt gttgccgcaa agtagtgcgg
     2941 gaaacatttt ccaggtcgga attgccacgg gaaagttcca cggcgttatc agcgcgcaga
     3001 cgccaaccgg catccgaacc gacatggcaa atttatccgg caactccgcc ggagtggttt
     3061 cgccaaaaag tcgccggcct tcgccggcaa cgtagaacgc cgtgtcgatt ccttcctgaa
     3121 catcaccgcg ggtctccttc aaaactttgc ccatttcccg agtcatgccg cgcgcgatct
     3181 cttccttatg attgcgcagc aattcaccca cacgatagag gatctcgccg cgctttgggg
     3241 cggggaccag acgccatgtg gcgaaggcgc tctttgctgc ttgcacagcg gcgtcgacat
     3301 cctccgccg
//
LOCUS       DASULZ010000434         2477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_763727, whole genome shotgun sequence.
ACCESSION   DASULZ010000434 DASULZ010000000
VERSION     DASULZ010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763727"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VFY96_08820"
     CDS             complement(<1..250)
                     /locus_tag="VFY96_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5606603.1"
                     /translation="MPTRDEPVQIPVDGQVLAGTVITPVTSLPALLFVHGWGASQQSY
                     LAHAREIAEMGLVCMTFDLRGHAHTDDQRETVSRDHNLR"
     gene            complement(234..1400)
                     /locus_tag="VFY96_08825"
     CDS             complement(234..1400)
                     /locus_tag="VFY96_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3182 family protein"
                     /protein_id="HEX5606604.1"
                     /translation="MICASHLNQKLSRHETKRAGGGVVLVCPAKDPNRNAQHENATRV
                     SVAKRLAVLKGYDFGGEYDAAIADWRRRYFVPADTLVGIEATRKLGILDEENFFGGLV
                     PYPFMATKTITHRLVNSSATAPEGWTENFASRVQSVVLLGFATFTLEDARLACARVLQ
                     SGDARLKPALCVGGQGQQVISTLNELDRALDCIDPMDLSTHGLSIEENLQNVTTYSVG
                     TVRLEEMRIAYCGTQRVTRDHHHKSVYGGSDLLVVPGDFDDLLKLNLASEFRAAVKKA
                     RVYDVAASTEIPGLIASRRNYDVAFGEDCHGRRRGGVLEQSWRIGGASPAEVAALEAF
                     REDPSLIRVRASCAEIYDARHEPPPHAQISFSGEDTDSGALIKYSVVERYADTR"
     gene            1885..2451
                     /locus_tag="VFY96_08830"
     CDS             1885..2451
                     /locus_tag="VFY96_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:electron acceptor oxidoreductase subunit
                     ActD"
                     /protein_id="HEX5606605.1"
                     /translation="MAGKNTAAFGIYHDEESLRTGINSLESAGFRATDISVLVPQNVG
                     TKDFAHEKASKAPEGAATGGGSGAVVGGVLGWLVGVGSLAIPGVGPFIAAGPILAALA
                     GMGVGSVTGGLIGALVGAGIPEYEAKRYEGMIREGGFLVSVHCDNSEWTSKAKEILER
                     TGADDVSSTGESGADFAESDKPRPRTLV"
BASE COUNT          557 a          657 c          706 g          557 t
ORIGIN      
        1 ctctgaggtt atggtcgcgt gatacagttt cgcgttgatc atcggtgtgc gcatgcccgc
       61 gtaagtcgaa cgtcatacag accaacccca tctccgcgat ctcgcgggca tgggcaagat
      121 agctctgctg gcttgcaccc catccgtgaa caaagagcaa cgcgggcaaa gaagtaaccg
      181 gcgtaatgac cgttccggcg aggacctggc cgtctaccgg tatttgcaca ggttcatcgc
      241 gtgtcggcat aacgttctac cacgctgtac ttgatcagag caccactatc agtatcttcg
      301 ccactgaagg atatttgggc gtgtggcggc ggttcatgcc gagcatcgta gatctctgcg
      361 cacgatgccc tcacgcgaat cagactggga tcttcgcgga atgcctccag agccgcaacc
      421 tctgctggac ttgctccgcc gatgcgccac gattgttcca ggacaccacc gcgcctgcga
      481 ccgtggcaat cctcgccgaa ggcgacgtcg taattccgtc tagaggcaat caatccggga
      541 atctcggtcg aggccgccac gtcataaacc cgtgcttttt tcacagccgc gcgaaattcc
      601 gatgcgaggt taagcttcaa aagatcgtcg aaatcacccg gcaccacgag tagatcggag
      661 cctccataga cactcttatg gtgatgatcg cgagtcacgc gttgcgttcc acagtaggcg
      721 atccgcatct cttccaaccg cacagtgccg acactatacg tagtgacgtt ttgcaaattt
      781 tcctcgatgc tgagcccatg agtcgacaag tccatgggat caatacaatc gagagcacgg
      841 tcgagctcgt tcagggttga aatcacttgc tgtccttgac cgccaacgca gagcgccggt
      901 tttagtcgcg catctcctga ttgcagcacg cgcgcacacg caagccgagc gtcttccagc
      961 gtaaacgtcg caaaccccag aagtacaaca ctttgcacac gggatgcaaa attctcagtc
     1021 catccttcag gagcagttgc actgctgttg actagccggt gagtaatagt tttagtggcc
     1081 atgaatgggt aaggaacgag accgccaaaa aagttttctt cgtccaagat tccgagcttt
     1141 ctcgtcgctt cgattccgac cagcgtatct gccggtacga aataacgcct gcgccaatcg
     1201 gcgatagcgg catcgtattc cccgccaaag tcatatcctt ttagaacggc caagcgcttg
     1261 gctaccgaaa cgcgtgtggc gttctcatgc tgcgcatttc tgtttgggtc tttcgcgggg
     1321 caaactaata cgactcctcc gcccgcccgt tttgtttcat ggcgagatag cttctggttc
     1381 aggtgacttg cacaaatcaa ttctgcgcct ctgcttgcgt cgcgcccagc actctttccg
     1441 agcggcatgg agcaaatcgg gtaccagggg ttctcggccc gcgtctgggg cacgggcagc
     1501 gtgaaccgtt gagacttgac ctgattgaag gatgtttacg ttggcaatga accctgttgc
     1561 gggatgagga aaataatatt gggctttgct tttcccgtcg cttgcgctcg gcgccggaac
     1621 aactggtcag cacgctcgca ctcaagacgc aatttagcgc gcagcgggtg aactattaag
     1681 cgtgctaacg ctgcacaaac gggtggcagt cttccgtagg gaaaaatcga attcagaaac
     1741 tttgccttgc agggaaatct cttctcgtct tacgtgattt gatcaaccgc tatgaaaagc
     1801 ctatacaatt cgcgcgaaac agcggtaggt cactccgggc atcttgcttg ctctgatgtg
     1861 tgatcaccag aaaggagaat agccatggca ggaaaaaata ccgcggcgtt cgggatatat
     1921 catgatgagg aaagtctcag gacgggtatc aatagtcttg aaagcgcagg ttttcgggcg
     1981 acggatattt ccgtattggt tccgcagaat gtgggaacta aagactttgc gcacgaaaaa
     2041 gctagtaagg caccggaagg cgctgctacg ggaggcgggt cgggggctgt tgtgggcgga
     2101 gtgcttggct ggcttgtagg cgtcgggtct ctcgcgatac caggtgtcgg acctttcatc
     2161 gccgccgggc cgatattggc ggcactggcc ggtatgggag ttggcagcgt caccggaggc
     2221 ttgattggag cgctggtggg agcgggtatt ccggaatacg aagcaaagag atatgaggga
     2281 atgatccggg agggcggctt tctagtctcg gttcattgcg acaattcgga gtggacatcg
     2341 aaggccaagg agattcttga gcgaaccggg gccgacgatg tctcttccac gggcgagtcc
     2401 ggtgcagatt tcgctgaaag cgataaacca cggccacgta cactggtctg agtcagagag
     2461 ataaacgaga gggatgg
//
LOCUS       DASULZ010000435         4528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_357381, whole genome shotgun sequence.
ACCESSION   DASULZ010000435 DASULZ010000000
VERSION     DASULZ010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357381"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..317)
                     /locus_tag="VFY96_08835"
     CDS             complement(180..317)
                     /locus_tag="VFY96_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606606.1"
                     /translation="MRSCSQMINSNILVVDDNSAIRKLLVRRLRESSYETVEAATGFK
                     R"
     gene            complement(962..1405)
                     /locus_tag="VFY96_08840"
     CDS             complement(962..1405)
                     /locus_tag="VFY96_08840"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX5606607.1"
                     /translation="MRRRWTDFQVDPRSGILDWYTVLTGPFAVATLTVHGAHYVALKT
                     EGDLSERARRAANQAWWPLVILIGLSLVASLYIRPHAIDNLLRIPGMADSGDGSGLFN
                     CAAALPLTEKDQAAFVASSVYFIATLGGRPARSPILLPRKHGSAL"
     gene            1809..2183
                     /locus_tag="VFY96_08845"
     CDS             1809..2183
                     /locus_tag="VFY96_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606608.1"
                     /translation="MKKSTSILTMSLLAGFLVIPSVRSYADDNPPGNHRQREALRRDQ
                     RDLERLRDQRRREIREGDRREAREYNDKIRDKKKEIWQDRREIYGNDHGRRYDRNGRY
                     GWRYGQGERYGYRDRHYDYNRD"
     gene            complement(2247..2483)
                     /locus_tag="VFY96_08850"
     CDS             complement(2247..2483)
                     /locus_tag="VFY96_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008345654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3072 domain-containing protein"
                     /protein_id="HEX5606609.1"
                     /translation="MTDKRTQQDADNSNMSKNPDEWVTGDEPMTGAQRSYLKTLSEEA
                     HEPFEENLTKAEASKRIDELQRKTGRGVGGSGSN"
     gene            complement(2558..2746)
                     /locus_tag="VFY96_08855"
     CDS             complement(2558..2746)
                     /locus_tag="VFY96_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606610.1"
                     /translation="MPSKKNKGGPHSIKSKKRKHSTGQNRGKQAKPPGETNQPFEQDS
                     KRRVGQHMGTGRPPLMKK"
     regulatory      3130..3214
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            3395..4048
                     /locus_tag="VFY96_08860"
     CDS             3395..4048
                     /locus_tag="VFY96_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606611.1"
                     /translation="MKATNANRETAKIRSSDTEQRPTHVPNANAINFARQLRALGQAL
                     ERFSFSAFEIEVENGIYSVVAKASATERKRTLSVFSAIGGFFRRSHATRTGPDRQVDL
                     RFTPEEIEKFDLHGRSRRQDAGKTPDPYSISQLLRGAGCYLDNRNVSERVTISFAGRW
                     ITIRYETPDKQAANARHDLEYFYDCWVQMYLRRSNRAKLPQASEPTVIMTWQGLRRS"
BASE COUNT         1075 a         1144 c         1263 g         1046 t
ORIGIN      
        1 gcggcgccag cggcaatagc acgattgaga acacttccct ggtgaaaagc tgtgagtacg
       61 attacgggta tgtctcgggt cgttggatcg gctgagcgat gcgatggctt aatcaccagt
      121 accggcgggc acaccaagat ccatgatgat cagatcggga cgggtcgttc gtgcttgccc
      181 taccgcttga agccggtcgc tgcttcaacc gtttcgtagc tggactctct aaggcggcgc
      241 accagtaatt ttctaatggc ggaattgtca tcgacgacga gaatgttgct gttgatcatt
      301 tgggaacagg aacgcatggc cccactctat aaaacctgtc catttgatca accagtttct
      361 ctggcctgct tttttggtcg tccccccact gatttggttt tgcgatattg agttagatcg
      421 gcgcgctgaa taaggcatac gcggcctaat ttgaggcatg gtaatcggcc gcgtttaatc
      481 gccttgtgga tagccgccgg agttatgttc aacagcttcg ccgcttgctg aacggtgacg
      541 agagtctgga tgtctatctt gggtgtcatt acgtgatgcc ggatcttgcc gtagcccagc
      601 cgagagctgg acctgagcat agctcatgcc ccaggtaccc atgggaattt cagagagact
      661 cctggcatgc gaggtaacaa agcgagcggc agcgcgatgg caagtttgcc tgaaaatgtt
      721 cgaagcagaa tttatacacg cggattgagg caaaaatttt tttcgacgtg ggctatgcgc
      781 agaaatagat gagcctcggc gtccgcgggt cactagccgt catcggtcag gtgggctttg
      841 ccgcggaaga accgaactag aaaagtaaaa tatacgatcg cgagcgcaat gccgacgagc
      901 cacaagatca actcgaggct gaagctatag acgccgtgtc ttcgcgttct cgatcgtgag
      961 gctatagcgc cgatccgtgc ttgcggggga gcaggatagg tgagcgcgca ggccgcccac
     1021 caagagtggc gataaaatag acgctggacg cgacgaatgc ggcttggtcc ttttccgtga
     1081 gcggcagagc ggcagcgcaa ttaaacaaac cactaccatc gccggaatca gccatccccg
     1141 ggatccgaag taaattgtcg atggcatgcg gccggatata gagactcgcg accaagctca
     1201 acccgatcag aatcacgagc ggccaccaag cctggttggc ggcccgacgt gcgcgctcgg
     1261 agagatcgcc ttccgttttt aatgcgacat agtgggcgcc atgcacggtg agcgtcgcca
     1321 cagcgaacgg cccagttaaa actgtgtacc aatcgagaat gccggaacga gggtcgacct
     1381 ggaaatctgt ccaacgtcga cgcagccaga gcgacgctca cgcaaaggtg acgaacatct
     1441 tgctgtgctc ctcttgagtt cggaaaagta gaaaaaactc taggctggcc aagataaacg
     1501 accccgtcac ctaagcccgc gcatctcgct atgttctcgc gcgttctcgc ttcagtcgaa
     1561 gcaacctaag gtaacgtcat gtacatcatc ggatcacgaa taacctgacc acaatgagcg
     1621 atagcatcaa tggcgggtgc ggtatgttca tgtttgcgac gaagtggaat gaaaaattag
     1681 gtatgagaat tcctccaacg ttataaatcc actaggcgga agacgcctat cttgctgtac
     1741 ttacccattg gtatggtgct tgcgaaaact tatgagcaca gcaggagatc acgagaagag
     1801 gagggaaaat gaaaaaatcg acttcgatat taactatgag tttactagcg ggttttctgg
     1861 taattccgag cgtgcgctca tacgctgatg ataaccctcc aggcaatcat cgacagcgtg
     1921 aggctttgcg acgggaccag cgggacctgg agcgattgag agaccaacgt cgccgcgaga
     1981 ttcgcgaggg cgaccggcgc gaagcgcgcg aatacaacga caaaattcgc gacaagaaga
     2041 aagaaatttg gcaagaccgt agagagattt acggaaatga ccacggtcga cggtatgatc
     2101 gcaacggtcg atacgggtgg cggtacggac aaggcgagcg ctatggatac cgtgaccggc
     2161 actacgatta caaccgcgac tagcctgtta cgcagagggt gcgcgagcag gaattagggg
     2221 cgactatccg tcgcccccta attcttttaa ttagatccgc tgccgccaac gccccgtccc
     2281 gttttgcgct gcaattcgtc gattcttttc gaagcttccg ctttggtgag attctcttcg
     2341 aacggttcgt gggcttcttc tgagagcgtc ttcaaatagg atcgctgtgc tccagtcatc
     2401 ggctcatcac ccgtgaccca ctcgtcggga ttcttgctca tattactgtt gtccgcgtcc
     2461 tgctgcgtgc gtttgtccgt cataaatgca ctccttactt tgaaacttcg ctatccaaaa
     2521 cgaaacttga tttggcgtcg cgtcgcctcc gctgggatta ctttttcatc aacggtggcc
     2581 gtccggtgcc catatgctga ccgacgcggc gcttgctatc ctgctcgaaa ggttgattgg
     2641 tttctccggg cggcttggcc tgcttgccgc ggttttgacc cgttgagtgc tttctttttt
     2701 tgctttttat actgtgcggg ccgcctttat ttttctttga cggcatagat cctcacttag
     2761 tcgttaatag ctgagtcccc gggaatgtgt gctcaaaaaa gtatagcact gggttcgtac
     2821 ggctccagcg tcattggcaa tttttccaga cgcgagtgaa ttcgaaccgc cgaagtcttc
     2881 cggcagagat agaggtccct aatgtgtggg atcaggtctg gcggctcgtg gttacgcggc
     2941 aatgatcggt tatgcgacag cctgcgaagc actgttaaat ttcttaacac gacagctgcg
     3001 acaactcact tgtcattgcc aggaagcttt ctttggaaaa ccctaagtcg acttgtcggt
     3061 agatggccat gattgttcca aaggatgcaa acgagcgcac tggcatcata tctgcgcaag
     3121 ctgcgatcag atttacgttg acgcaatgaa ccgaaatccc aacgtggtca ccatgggact
     3181 cggcgagcga gtggtgaaac cggcaacaga aaactctctg ggccggtttt tttttgatgt
     3241 gcagcgaaca acgatgaaag gacaaccatg aatccggtgg gaggaaaacg gtcagcgctt
     3301 aacggagtac atgttggtga tcgttctggt accgcctaat ctctaccgta cttgaccggc
     3361 aatgaggcat aactcatagc agaaacgagt ttatatgaaa gccactaacg cgaaccggga
     3421 aacagccaaa atacgctcgt ctgacaccga gcagcggccg actcatgtgc caaatgccaa
     3481 tgcgatcaat ttcgcgcgcc aacttcgcgc gctcgggcag gctctggaaa ggttcagttt
     3541 ctcggcgttc gagatagaag tcgagaacgg aatctattcc gttgttgcca aagccagcgc
     3601 gacagagcga aaacgaacgt tatctgtttt cagtgcgatt gggggatttt ttcggcgctc
     3661 ccatgcgact cgaaccgggc ctgatcgcca ggtggatttg cgctttacgc cggaagagat
     3721 cgaaaaattc gacttgcatg ggaggagtcg gcgccaggac gccggcaaaa cgccggaccc
     3781 atacagcatc tcacaattgc tgcgcggcgc gggatgctat ctcgataatc gaaatgtgtc
     3841 ggaacgggtc accatctcat tcgccggccg atggatcacg attcgatatg aaactccgga
     3901 taagcaggct gcaaacgcgc gacacgacct tgaatatttc tacgattgct gggtacaaat
     3961 gtatttgcgc agaagcaatc gtgcaaaatt gccgcaggcc agtgaaccaa cggtgattat
     4021 gacatggcaa ggtctacgtc gttcgtaggc cttggtttag gcggttccgt gcggattcct
     4081 cgcggaagcc agaagtccca ctgccgatgc atgaggcctg atcacggcgg aggtcgctgg
     4141 tcccgctaag tccagcggct ggatgggatc gtgcgatgcc atgcgtccgc acttgggcag
     4201 agttgttgtc ggaattttcc cgccggtaaa tcgccgttcc tagaggattt tggcagatca
     4261 aaaagcccca ttcatccgcc taagtctccg gtttatctac acggaatcga atttgctcgg
     4321 ctctatagcc gccatgacgc gcggacaaga tagtttcggc tgtgagttgg taatggcgac
     4381 aggcagacaa tcagccggac ggccgctgca tggacttaaa aaagcatctt ggaatttgaa
     4441 ttggggtttt tatgagattc gacttgtaac cttagagtcg ttcgattatt ggtgaacggg
     4501 tgccgttcgg aaagggccag gatccaaa
//
LOCUS       DASULZ010000436         2160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1373998, whole genome shotgun sequence.
ACCESSION   DASULZ010000436 DASULZ010000000
VERSION     DASULZ010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373998"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VFY96_08865"
     CDS             complement(<1..467)
                     /locus_tag="VFY96_08865"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5606612.1"
                     /translation="MISNGSILVAEDQESARESLVELFKDEGYDVYEAPDGNAAISMI
                     DKLDLDLVLTDLMMPGADGIAVMKHVRDVSPQTLVMIMSAHATVETAIQAIRLGAQDY
                     LLKPLIFDEVLRKTRQLMEHRELAWENQLLRREINVHFSSERPLGRSHAMQELS"
     gene            complement(593..>2160)
                     /locus_tag="VFY96_08870"
     CDS             complement(593..>2160)
                     /locus_tag="VFY96_08870"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5606613.1"
                     /translation="IKESARQRALADLSGHALDDADLAALMAIITELIAKTLNVEFCK
                     VLELLPGKDNLLLRAGIGWTDGLVGQATVPVGAESQAGYTLLCNEPVIAEDLRTERRF
                     SAPSLLRDHRIISGISVRIGRSDRPFGVLGAHSSATRTFSKDDVQFLQIAANFLSQVI
                     ENKRALEEVRREAIWIERLIETTQDAVISIDRRGCIVLFNAAAERTFGYSAGEIVGQK
                     VNVLMAEPYATEHDGYLERYEKTGEAHAIGRIRTVTAKRKNGELFPIELSVTKITEDR
                     EVQYAAFIRDISEKARLQAQLVENERLITIGTTAAKIGHELANPLNGMSLTIQLLEQR
                     LNKQSSLVDAQITSTVNRLRNEVARLNTLLTQFRSLSRREKFDFQRTTLTALVGEAIE
                     IEMPRYAESGIEVECSFPSNLPALTVDIDKMKQVILNITKNAAEAMAGGGKISVTGSA
                     SQNGIVLEISDTGTGIPAGINVYEPFFTTKSQGTGIGLSIVQQIVNAHGGTVSYRSEH
                     GKGTTFTITLPYR"
BASE COUNT          488 a          592 c          542 g          538 t
ORIGIN      
        1 gagagttcct gcatggcgtg gctgcggccc agcggtcgct cggaactgaa atggacatta
       61 atttcccgcc gcagtaactg attctcccac gctaactccc tatgctccat caactggcga
      121 gtcttgcgca gaacctcatc aaatatgagc ggcttaagca ggtaatcttg cgcgcccaaa
      181 cgtatcgcct gtatcgccgt ctcgaccgtg gcgtgcgccg acatgatcat cactaaagtt
      241 tggggtgaga cgtcacggac atgcttcatc acggcaatgc catccgcacc gggcatcatc
      301 agatccgtta gaactaaatc taaatcgagc ttgtcgatca tcgagattgc cgcgtttccg
      361 tcgggcgcct cgtaaacgtc gtaaccttcg tccttgaaca actcgaccag gctttcccgt
      421 gcactttcct gatcttcagc gactaatatg gagccattcg aaatcatttt acagttgccc
      481 tattccttaa tatgcgaaaa tatagatgcg atttttgcca caaatgcaaa ataaaccgct
      541 tggtctatga gaaatgattt acattctagg atttcgcaat tttggcgcca ttctatcggt
      601 agggcagggt aatggtaaaa gttgttcctt ttccgtgctc gcttctatag ctgacggtgc
      661 ctccatgagc attgacaatc tgctggacga ttgaaagccc aatacctgtg ccttgagatt
      721 ttgtcgtaaa aaacggctca taaacgttga tacctgcggg aattcctgtg ccagtgtccg
      781 aaatttcgag aacgattcca ttctgggaag ctgagcccgt aactgaaatt ttcccgccgc
      841 cagccatcgc ctctgccgcg ttcttggtaa tattcaagat cacctgtttc attttatcaa
      901 tatcaaccgt gagggccggc agattcgagg ggaaggaaca ttctacttcg attccggatt
      961 cggcataacg cggcatttca atttcaattg cttcacctac tagagcagta agagtcgtcc
     1021 gctgaaaatc gaatttctcg cgccgcgata gagaacgaaa ctgtgtgagc aaagtattca
     1081 gacgcgcaac ctcgttgcgc agtcgattca cagttgaggt tatctgcgca tcgactaggc
     1141 tcgattgttt attgaggcgt tgctcgagaa gctggatagt caacgacata ccgtttaatg
     1201 gattcgcaag ctcatggccg atttttgccg ctgtcgttcc gatggtgatc aaccgctcgt
     1261 tctccacgag ttgtgcctgc agccgagcct tttctgaaat gtcgcgaata aacgcagcgt
     1321 attgaacctc ccggtcttca gtgatctttg tcaccgatag ctcgatcgga aacagctcgc
     1381 catttttgcg cttggcggtc accgttcgaa tgcggccgat ggcatgggct tcgcccgtct
     1441 tctcataacg ttcaagatat ccatcgtgct cggtagcgta cggctcggcc atgagaacgt
     1501 tgaccttttg cccgacgatc tcgcccgcgc tgtagccaaa ggtgcgctca gcggcggcgt
     1561 tgaacaacac gatgcaaccg cgccggtcga tggaaatgac cgcgtcctgt gtcgtttcga
     1621 tgagtcgctc gatccagatc gcctcgcgcc ggacttcttc caaagcgcgc ttgttctcga
     1681 tcacttgtga gagaaaattg gcggcgatct gcaggaattg aacatcatct ttagagaacg
     1741 tccgcgtcgc cgagctatgt gctccgagga caccgaaagg ccggtcgctg cggccgatac
     1801 ggacactgat gccactgatg atgcgatgat cacgcagcaa ggaaggcgcg ctaaacctcc
     1861 tctccgtacg aagatcttcg gcaatcacgg gttcgttgca aagcagagtg tagcctgcct
     1921 gcgattcggc acccaccggc acagtggctt gtccaaccaa accgtctgtc cagccgatcc
     1981 ccgcgcgtaa cagcaaattg tccttgccag gcaaaagctc caagactttg caaaattcga
     2041 cattgagggt tttggcgatc agctcggtga tgatcgccat gagcgctgcc agatccgcgt
     2101 cgtccagagc atggccgctg agatcggcca aggcccgctg ccgcgcgctc tctttgattg
//
LOCUS       DASULZ010000437         2764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_562087, whole genome shotgun sequence.
ACCESSION   DASULZ010000437 DASULZ010000000
VERSION     DASULZ010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562087"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..851
                     /locus_tag="VFY96_08875"
     CDS             21..851
                     /locus_tag="VFY96_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX5606614.1"
                     /translation="MSLLAPFEYEFFRNGLIAAVVIGALCGFIGVYVVLRRMSYIGHG
                     LSHAIFGGAVLSYVWNINFFIGGGLWALLSGVLIHWISHRRWVGADAAIGVVTTSSFA
                     LGVAIISTHRSFTRNIEAALFGNILGITQRDVWIVVLVSLLVAIVLFLIRRPLLFATF
                     DPEVASAYGVATGKIDLVFILLLAATVLASTQILGVTLVAAAMVIPPVIARYMTQRFQ
                     SLLILSPVLGAFCGAAGMFLSFYFDISSAATIVLTAAALFLVLEIGLLARGKKRPPTT
                     "
     gene            963..1391
                     /locus_tag="VFY96_08880"
     CDS             963..1391
                     /locus_tag="VFY96_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606615.1"
                     /translation="MKILNLVCAFGLMLTAARIVTAAQESHDDHIKGMDMPMKHSDKI
                     PPKSALKPAQGASVKIISPKAGQVIKGDSVPLEFKLTKGKIGEHVHAYVDGEMAGMFQ
                     SSKGTLNGIKPGQHTLELRVATADHNTELNAMDKVTFTTK"
     gene            1497..2090
                     /locus_tag="VFY96_08885"
     CDS             1497..2090
                     /locus_tag="VFY96_08885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016875240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5606616.1"
                     /translation="MRQRKFKRFCLCFVVIISVGFTEVYEPVFGTGGLARAQDSSFQS
                     KKIVPFVPSPQEVVDKMIELGGVKPGDVVYDLGSGDGRIVVAAAKRGAKAVGFEIDGD
                     LVKQSQENIRAAGVQDAAEIRQQDILTVDLSPASVVTMYLLPDVNLKLRPNLLSQLKP
                     GSRVVSHSFDMGDWKPDKIERVEGRTIYLWIIPAKAR"
     gene            2133..>2764
                     /locus_tag="VFY96_08890"
     CDS             2133..>2764
                     /locus_tag="VFY96_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606617.1"
                     /translation="MPRSAKNIFLLLLTIAVLILPAIYAIQTVGNRRPEQPIQTLSSY
                     LHFLYARDFRRAYRFIATEDQRVKPLSDYLRERPPFTGFTLDVARKLAALIEIRPLSE
                     APEQNLNRVKVNLKLPDANALSGLLLDWDESRLNGLPALEQKKILAAIDELNRQKKLP
                     TIEGEEEFTLLREGSQWRISLDWGAGVKVSFTTVLPSETAIFAEPVAKETV"
BASE COUNT          596 a          778 c          726 g          664 t
ORIGIN      
        1 tttgaaccat ttgaactgta atgtctttac tcgctccgtt tgagtacgaa ttctttcgca
       61 acggcttgat cgctgcggtc gtcatcggcg cgctttgcgg ttttatcggc gtatacgtcg
      121 ttctgcggcg catgagctac atcgggcacg gcctgtcgca cgcgatcttc ggcggggcgg
      181 tgctgagcta tgtctggaac atcaattttt tcatcggcgg cgggctctgg gcgttgctgt
      241 ccggggtttt gattcactgg atcagccacc gccgctgggt cggcgccgac gccgccatcg
      301 gggtcgtaac gacctccagc ttcgcgctcg gcgtcgcgat catctccacg caccggagct
      361 tcacgcgtaa tatcgaagcc gcgctctttg gcaatattct gggcatcacc cagcgcgatg
      421 tctggatcgt cgtgttggtc tcgctgctcg ttgccatcgt cctttttctc atccgccggc
      481 cgctcctctt cgccactttc gatcccgaag tcgcctcggc gtacggcgtt gccaccggca
      541 aaatcgatct tgttttcatc ctgcttctgg ccgcgacggt tctcgcttcg acgcaaatcc
      601 tcggcgtcac cctggtggcc gccgccatgg tcattccgcc ggtgatcgca cgctacatga
      661 cccagcgctt tcaaagcctg ctgatcctct cgccggtgct cggcgcattc tgcggcgccg
      721 ccggaatgtt tttgagcttc tatttcgata tttcgtcggc cgccaccatc gtgcttacgg
      781 cggcggcgct attcctggtc ttggaaatag gcttgctcgc gcgaggcaag aaacggccgc
      841 ccacgactta atattcattg agccatcgat gttcagcttg agcctcaggc gcgggaatat
      901 agtgggtcgg cactggagat cggcgcgatt ttatcgtcaa atagcgtgtg gagaggagaa
      961 tcatgaaaat tttgaatcta gtctgtgcct ttggacttat gctcactgcc gcccgaatcg
     1021 tcacagctgc tcaagaaagt catgatgacc acatcaaggg catggatatg ccgatgaaac
     1081 actccgataa aatcccgcca aaatcggcgc tcaagccggc tcaaggcgcc agtgtcaaaa
     1141 ttatttctcc aaaagcgggg caagtcatca aaggagactc cgtcccgctc gagttcaagc
     1201 ttacgaaggg caagattggc gagcacgttc acgcctacgt cgacggcgag atggccggta
     1261 tgttccaaag cagcaaagga actttgaacg gtatcaagcc ggggcaacat accctcgagc
     1321 tcagagtcgc caccgccgat cacaacaccg aacttaacgc gatggacaaa gtgactttta
     1381 ccacgaaatg agttagtgac tttttggttc aatccgacgc gtcaataacc cgaatctagc
     1441 gctcattcgg tttttatgtt taattcgaac cgatgccgga gcttcatcga aacccaatga
     1501 gacaacgaaa gttcaaacgg ttttgccttt gcttcgtagt tatcatttcg gttggcttca
     1561 ccgaagtcta tgagccggtg ttcggtactg gcggcttggc gcgcgcacag gactcgtcct
     1621 ttcagtccaa aaaaattgtc cccttcgtgc cgtcgccgca ggaagtcgtc gacaagatga
     1681 tcgagctagg gggcgtcaag cccggagatg ttgtctacga tctcggctcc ggcgatggcc
     1741 ggatcgtcgt cgctgcagcg aaaagaggcg caaaggcggt cggcttcgaa atcgacggtg
     1801 atctggtcaa acaatcgcaa gaaaatattc gagccgctgg cgtgcaagac gcggcggaga
     1861 ttcgccaaca ggacattctg accgtcgatt tgagcccagc gtctgtggtg actatgtatc
     1921 ttttgccgga cgtgaattta aaactgagac cgaatcttct tagccaactg aagccgggct
     1981 cgcgcgtggt ctcacattcc ttcgacatgg gcgattggaa acccgataag atcgaacgcg
     2041 tcgaggggcg cacgatttat ctctggatca ttcctgcaaa ggcacgctaa tcgccgacaa
     2101 gactgccgcc gattccactc tgagcgcaag aattgccccg gtccgctaaa aatatttttc
     2161 tgcttctgct cacaattgca gttctgattc tgcctgctat ttacgcaatt cagactgtcg
     2221 gcaacaggcg tcccgagcag cccattcaga cactatcaag ctatcttcat tttctttatg
     2281 ctcgcgattt tcgtcgcgcc tatcgcttca ttgccactga agatcaacgc gtgaagcctc
     2341 tgagcgatta cttgcgcgaa cggccgccct ttaccgggtt tacgttggat gtagcgcgaa
     2401 aacttgctgc gctcatcgaa atccgtccgt tatcggaggc gccggagcaa aaccttaacc
     2461 gagtcaaagt gaacctcaag cttccggatg ccaacgcgct ttccggcttg ttactggact
     2521 gggacgaatc gcgactcaac ggcttgcccg cgctggagca aaaaaaaatt ctcgcggcca
     2581 tcgatgaact taaccgtcaa aaaaaattgc ccaccatcga aggcgaagaa gaattcactt
     2641 tattgcgtga aggctcccaa tggcgaattt ctctcgactg gggcgccgga gtaaaggtga
     2701 gtttcacgac cgttttgcca tcggaaaccg ccatatttgc ggaacccgtt gccaaagaaa
     2761 cggt
//
LOCUS       DASULZ010000438         3382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_257171, whole genome shotgun sequence.
ACCESSION   DASULZ010000438 DASULZ010000000
VERSION     DASULZ010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257171"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VFY96_08895"
     CDS             <1..342
                     /locus_tag="VFY96_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5606618.1"
                     /translation="IVNIASGTVFLGAPGRIHYVTSKAETIGFTRTLAREVGDDNITV
                     NCLAPGNTLSEENPTEQMIKFRESSIGARSLKRIQLPQDVVGAMLFLASPLSDFITGQ
                     TINVDGGISFL"
     gene            434..1474
                     /locus_tag="VFY96_08900"
     CDS             434..1474
                     /locus_tag="VFY96_08900"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5606619.1"
                     /translation="MNVDYPIIDADGHVLEKDRELHDYLGGRYSSAPRFETYSYFPSL
                     DGWNRGTGVPGKDPETPAPRWLQFLDELGVHTTVLYPTGGLGLGLVQNPEWACVLARA
                     YNSWLAERFSSVSPRLKGVALLPVHEPVEAAKELARAKSLGLVAGLMPAVTWLHKGYG
                     HSDFDPIYETAERLDMPLTVHGAPSRGMGFDFFNKFLHVHTLEHPFAILIQFTHMVFE
                     GVFVRFPKLRVAFLEAGSGWLPYMMDRMDEEMEKPYRVQAPLLKQKPSELIRNGQIWT
                     TCEVEEKALPQVLKQFNPKCLMWPSDYPHERLPDMFQRDLPEFLERDDLSDASKKAIL
                     HDNPIDFYRLDI"
     gene            1580..2338
                     /locus_tag="VFY96_08905"
     CDS             1580..2338
                     /locus_tag="VFY96_08905"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX5606620.1"
                     /translation="MRLKDKVAIVTGGGVGIGKAYAHGLAKEGAKVVVADIQDGEAKK
                     VAADITSAGGDALPVYVDVTSMEKTQAMAQAALSAYGRIDILVNNAGLYSALKKKNFM
                     EIDSDEWDRVMTVNVKGLWHCVKAVYPAMKQQGKGKIINISSGTVLGGSPFFLHYVSS
                     QGAVFGFTRALAREIGDDRICVNSIMPGLTISSANQEGVMTAEQLADRRKRRAFHRDQ
                     YPADLVGTVIFLASDDSDFMTGQSISVDGGMNMH"
     gene            complement(2352..>3382)
                     /gene="amrB"
                     /locus_tag="VFY96_08910"
     CDS             complement(2352..>3382)
                     /gene="amrB"
                     /locus_tag="VFY96_08910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEX5606621.1"
                     /translation="ASFVAMLDEHYYLDSPRFRQHQSAAILEFRRQTTRAAAHVGGVY
                     KAVPTELKKQLEGYFVAPEGPGLPAARNSVQRPSAIVAPHIDFHRGGPAYAWAYKTLV
                     ESDGADLYILLGTSHCSGHMPYILTCKNFETPLGTVETERDFVQQLHAKCTDDFFAEE
                     YLHRGEHSLEFQVLFLKYVAQQRAALRGESERPFKIVPILVSSFHSAVLEQTLPEKTS
                     MVGKFLDGLRELLSQERRELCLVAGVDLAHVGKQFGDRQPITEDFLKWVESEDQLLVE
                     RLIALDAQGFFHEIAKDQDRRRICGFSPLYSLIHLLDGSRGTQLKYSQAFTPETGSAV
                     TFTSLVFY"
BASE COUNT          766 a          934 c          941 g          741 t
ORIGIN      
        1 atcgtcaaca tcgcatcagg aacggtgttc ttgggggcgc cggggagaat tcactacgtg
       61 acttccaaag cggaaaccat cgggttcacc cgcactctgg cccgcgaggt cggcgatgac
      121 aacattacgg ttaactgcct cgctccgggc aacaccttgt ctgaagaaaa tcccaccgag
      181 cagatgatca agtttcgtga atcatccatc ggcgcgagat ctctaaagcg aattcaactt
      241 ccccaagacg ttgtcggcgc aatgctgttt ctggcctcgc ccctgagcga ttttatcacg
      301 ggtcaaacga taaacgtcga cggtggaata tcatttttgt gaactggagt cagtcgcgag
      361 agcgttgcgc tcccttgggt ccgaattcga tttgaacgtt ttgcgggctt gaacgatttg
      421 aacggagaac aatatgaacg tcgactatcc gatcatcgac gccgatggcc acgttttgga
      481 aaaagaccgc gagctgcacg attatttggg cggcagatac tccagtgcgc cccgctttga
      541 aacctattca tactttccgt cgctcgatgg ttggaatcgg ggtaccggcg tgccgggcaa
      601 agacccggag acgcccgcac ctcgttggtt gcagtttctc gatgagctcg gcgtgcatac
      661 aacggtgctc tatcccaccg gcggcttggg gctcggcttg gtgcaaaatc cggaatgggc
      721 ctgcgttctc gcccgtgctt ataactcctg gcttgccgag cgcttctcta gcgtcagccc
      781 acgcctcaaa ggcgttgcct tattgccggt gcacgagccg gtggaagccg ccaaagaatt
      841 ggcccgagca aaatcgctcg gcctcgttgc cgggctgatg ccggcagtaa cttggttaca
      901 taagggctat ggtcacagcg actttgaccc catttatgaa accgcagagc gcctcgacat
      961 gccgctcacg gtgcacggcg cgcccagccg cggcatgggc ttcgattttt tcaacaagtt
     1021 tctccacgtc cacacactgg aacatccgtt tgcgatcctg atccaattca cgcacatggt
     1081 ttttgagggc gtctttgtgc gttttccgaa gctgcgtgtg gcatttcttg aagccggctc
     1141 cggttggctc ccttacatga tggatcgcat ggatgaggaa atggagaagc cctatcgagt
     1201 tcaggcaccg ttgctcaagc aaaaaccgag cgagctgatt cgtaatgggc aaatctggac
     1261 gacctgcgag gtcgaagaaa aagctctgcc ccaggtgctc aagcaattca atccgaaatg
     1321 cctgatgtgg ccgtcggact atccgcacga gcggctgccc gacatgttcc agcgcgacct
     1381 gccggaattc ctcgagcgcg acgatttaag cgatgcatcg aagaaagcga tattacacga
     1441 taacccgatc gatttttatc ggctagacat ctgagcgcga agcatgattt gtggaatgtt
     1501 ctccaatgat catgaatcgc attatgtcat ttcgaccgga gggagaaatc gttccgatct
     1561 ttaacgcgcg gaggattcca tgcgactcaa agacaaagtg gcaatcgtca ccggcggcgg
     1621 tgtcggcatc ggcaaggcgt acgctcatgg cttggcgaaa gaaggagcga aagtcgttgt
     1681 tgcagatatt caagacggcg aagccaaaaa agtcgccgcc gacatcacat ccgccggggg
     1741 cgatgcgcta ccggtctatg tcgacgtgac ctcaatggag aaaacccaag cgatggcgca
     1801 agccgcgctt tcagcctacg gccggatcga catcttggtc aacaacgccg gactctattc
     1861 agcgctcaag aagaaaaact ttatggagat cgatagcgat gagtgggacc gcgtgatgac
     1921 ggtgaatgtg aaaggcttgt ggcactgcgt caaagcggtt tacccggcga tgaaacaaca
     1981 gggcaaaggc aagattatca atatttcctc gggtacggtt ctcggcggct cgcctttctt
     2041 tcttcactat gtctcatctc aaggagccgt gttcggattc acccgggcct tagcgcgcga
     2101 aattggcgac gaccgcatct gtgtcaattc gatcatgccc ggtttgacca tttcgagcgc
     2161 caatcaggaa ggcgtcatga cggccgagca gctggcggat cgcagaaagc ggcgcgcctt
     2221 tcatcgcgat caatatccgg cggatctcgt cgggacggtt atttttctcg cgtccgatga
     2281 cagcgatttc atgaccggtc agtccatcag cgtcgatggc ggcatgaaca tgcactgatg
     2341 cgatggactg cttagtagaa gactaaactg gtaaaggtta ccgcggagcc ggtctctgga
     2401 gtgaatgctt ggctgtattt cagttgagtg ccgcggctgc cgtcgagaag atgaatcaag
     2461 gaatagagcg gcgagaagcc gcagatccgg cggcgatcct gatccttggc aatctcgtga
     2521 aaaaatccct gcgcatcgag agctatgagt cgttcgacca acaactgatc ttcgctctcg
     2581 acccatttga gaaagtcttc cgtgatcggt tgccgatcgc cgaattgctt gcccacatgc
     2641 gcgagatcga cccctgcgac caagcagagt tcccgccgtt cctgactgag cagctcgcgc
     2701 agcccatcca agaactttcc caccatcgag gttttctccg ggagcgtctg ttcgagcact
     2761 gcggaatgaa atgaggagac taagatcggc acgatcttga acggtcgttc cgactcgcct
     2821 cgcagagccg ctcgctgctg cgcgacgtat ttgagaaata aaacctgaaa ttccaacgag
     2881 tgctcgcccc ggtgtaaata ctcctcggcg aaaaagtcat ccgtgcactt ggcgtgcaat
     2941 tgctgcacaa aatcgcgctc ggtctcaacc gtgccgagcg gggtttcgaa attcttgcag
     3001 gtgagaatat acggcatatg accgctgcag tgagaggtgc cgagcaggat atagagatcg
     3061 gcgccatcgc tttccaccag cgtcttatag gcccaggcgt acgccggccc accgcggtga
     3121 aaatcgatgt gcggcgcgac gatggcgctc ggtctttgca ccgaattccg tgcagccggc
     3181 aaacccggcc cctcgggcgc aacaaaatat ccctcgagct gctttttcag ctccgtcgga
     3241 accgccttgt aaacgccgcc cacatgcgcc gccgctcgtg tagtttgccg gcggaattcc
     3301 aagatcgcgg cactttgatg ctgacgaaac ctcgggctat cgagatagta atgttcatcg
     3361 agcatggcga caaaactcgc ca
//
LOCUS       DASULZ010000439         4706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_358196, whole genome shotgun sequence.
ACCESSION   DASULZ010000439 DASULZ010000000
VERSION     DASULZ010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358196"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..225)
                     /locus_tag="VFY96_08915"
     tRNA            complement(150..225)
                     /locus_tag="VFY96_08915"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(190..192),aa:Ala,seq:cgc)
     gene            complement(228..1091)
                     /gene="galU"
                     /locus_tag="VFY96_08920"
     CDS             complement(228..1091)
                     /gene="galU"
                     /locus_tag="VFY96_08920"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEX5606622.1"
                     /translation="MKLKKAVIPVAGLGTRFLPATKTVPKELLPIVDIPSIQYVVQEA
                     VDAGIEEIIFVTGRGKDGIEDHFDEAPELEQVLAERGNDEMIKMLRRIAEMTEVVSVR
                     QKKPLGLGHAVLCARDLVGNEPFAVMLADDLIDSATPCIRQLLDIFQSTNESVIALME
                     VPQTEVHQYGVIKGKVIKERLYEVEGMVEKPPAKDAPSRMAIIGRYVLRPEILPILQN
                     LPAGKGGEIQLTDGLGQLVRERKVYGCEFAGDRYDIGDKFGFVRATVAYALKRPDLKD
                     KVLEYLKSAKY"
     gene            complement(1111..1476)
                     /gene="dksA"
                     /locus_tag="VFY96_08925"
     CDS             complement(1111..1476)
                     /gene="dksA"
                     /locus_tag="VFY96_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEX5606623.1"
                     /translation="MNPSDLDFFRRLLNQRISELRFEAGKTVENMDQDVNFPDPTDRA
                     SLEASRNSVLRIRDRERKLIFKIQEALQRLDDGEYGICEECGEEIGIGRLKARPVTTL
                     CITCKSSQEIEERKAKRVV"
     gene            complement(1578..2399)
                     /locus_tag="VFY96_08930"
     CDS             complement(1578..2399)
                     /locus_tag="VFY96_08930"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5606624.1"
                     /translation="MKRRGFLGVVGSAWLLWLRGVAEAMCNPSLVELFHHGRHLVQAR
                     GNGAKAPSLGSYFVNWFGHSSFLIQSGSHTKVVADPNFNVTPGIQADAVTVSNDHFTH
                     NNTGAVTGDPVILRGITFKQTWNPIRTSIKDITIVNIPSQRSASWGAIANSIFIYEMG
                     SLCLAHLGNIGHLLTAEQVKVLQHVDVMMIPIDAMTNLAFEDIIKVIEQVKPPIVIPM
                     HYDVVRQGELFATFARDHYPVKRISQSQLTLNRGMLPKSTEVYILQHPRPSSFGE"
     gene            complement(2396..2956)
                     /gene="amrA"
                     /locus_tag="VFY96_08935"
     CDS             complement(2396..2956)
                     /gene="amrA"
                     /locus_tag="VFY96_08935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HEX5606625.1"
                     /translation="MPDVYLPFDSQRRLIKTARQTLEAITRGNQDGEPGESDPHLDCI
                     NYGAFVTLFNHHLLRGCVGTCAPSSGLFNIVIEMTEAAATCDRRVKPVCVDELKDIQI
                     HISVISPLDPADHPLHLEIGKHGIYIARHGKRGVLLPQVAVEHGWDGSAFLEQTCRKA
                     NLPSNAWRWPGTQVSLFTSLEIEEEK"
     gene            complement(3028..3420)
                     /locus_tag="VFY96_08940"
     CDS             complement(3028..3420)
                     /locus_tag="VFY96_08940"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX5606626.1"
                     /translation="MAKTRKQARTTKQLGTIWETIKSDKSHPTADQIYDRVRKKLPSI
                     SLGTVYRNLQKLVADQKLQVLMRGRSQHFDPLVQKHQHFICEICDRVYDVVLQNQREI
                     EPVKLPHEGFRVTSHQLAFYGICKHCSK"
     gene            complement(3422..3997)
                     /locus_tag="VFY96_08945"
     CDS             complement(3422..3997)
                     /locus_tag="VFY96_08945"
                     /inference="COORDINATES: protein motif:HMM:NF023432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HEX5606627.1"
                     /translation="MKKIVLDDILGLVAYEKVREEFRKDIIEKKKSRRLAVGAKVSLV
                     FENRDTVIFQIQEMLRAEKISDLDKIREEVAVYNELIPNSGELSATLFLELEDQSHLR
                     DDLLKFLGIDECVSLSISGRSLPARFESGRSKADKVSAVQYVRFCFDEEARRAFIAGE
                     RAELIIHHPNYEARTILAPEAQQSLAEDLAS"
     gene            complement(4105..4530)
                     /locus_tag="VFY96_08950"
     CDS             complement(4105..4530)
                     /locus_tag="VFY96_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013874387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEX5606628.1"
                     /translation="MGKSLAGTKSHENLKHAFAGESQANRRYLYFARVADIEGYPDVG
                     GLFRDTSEAETGHAFGHLDFLKEVGDPCTGVAIGTTAKNLKSAVEGETYEYTEMYPGF
                     AKTAREEGLPELAEWFETLAKAEKSHAGRFSKGLDTLGS"
BASE COUNT         1081 a         1303 c         1149 g         1173 t
ORIGIN      
        1 tgaaccgatc cagatccaat tcgtcatccg ttcctccgtg cgggctgaag gtttctacaa
       61 tttattaccc gttcctattt tcacagtgcc aggcatatgt caatgccgcc aaaaaaaagg
      121 gcgggtaaat aacccgccct cgcattattt ggtggagccg atgggagtcg aacccacggc
      181 ctctagagtg cgattctagc gctctcccaa ctgagctacg gccccattta atattttgct
      241 gattttaaat attcaagcac cttatctttt aggtccggcc gtttcaacgc gtacgctaca
      301 gtggcgcgaa cgaatccgaa cttatcgccg atatcgtacc gatcaccggc gaactcgcag
      361 ccgtaaactt tgcgctcgcg cacaagctgg cccaaaccat ccgtcagttg aatctcaccg
      421 cccttgccgg cgggaagatt ttgcagaatc ggaaggattt ccggacggag cacgtatcgt
      481 ccgataattg ccatccgcga gggagcgtcc ttagcgggcg gtttttcaac catgccctcc
      541 acttcgtaaa gtcgctcctt aatcacttta cctttgatga cgccatactg gtgtacctcc
      601 gtttgaggca cttccatcaa ggcgattaca gattcattgg tgctctggaa aatatcaagc
      661 aattggcgaa tgcacggcgt cgcgctgtcg atcagatcat ccgcgagcat aacggcaaac
      721 ggttcgttgc cgacgagatc acgggcgcag aggacagcgt gtcccaagcc cagtggtttt
      781 ttctgccgaa cggaaaccac ttcggtcatt tccgcgatac gtcgcagcat cttgatcatt
      841 tcgtcattgc cacgctctgc gagcacttgt tcgagctccg gcgcctcatc aaaatggtct
      901 tcgataccgt ctttgccacg accggtaacg aaaataatct cttcgatgcc cgcgtcgacc
      961 gcttcctgca cgacgtattg aatcgacggg atgtcgacga tcggcaacaa ttccttcggc
     1021 accgttttgg ttgcgggcag aaatcgggta ccaagtcctg caacgggtat aacagccttt
     1081 ttaagcttca tgagattgtt tcgacaagtc tcaaaccacc cgcttcgctt tccgttcctc
     1141 gatttcctgg ctggatttgc aggtaatgca aagtgtcgtc accggccgcg ctttcaaccg
     1201 tccgatgccg atttcctcgc cgcactcttc gcaaatgcca tattcgccgt catcgagccg
     1261 ttgcaaggct tcctgaattt tgaaaatgag cttgcgctcg cgatcacgaa tgcgcagaac
     1321 ggaattccgg cttgcttcca gagaagctcg gtccgtcggg tcgggaaaat tcacgtcctg
     1381 atccatgttc tccacggtct tgcccgcctc aaatcgcagc tcgctgattc gttgattcaa
     1441 tagccgacga aaaaaatcaa gatcgcttgg gttcaagttt caccttttca ccgaaaacga
     1501 acaatgccgt tattatagga gaaagtaggc aaacatcaac cggcggagtc agaattcgtc
     1561 cggcatgttg aacaacgtta ctcgccaaat gagctcggcc gaggatgctg cagaatatac
     1621 acctctgtgg acttcggcag catgccgcga ttgagggtaa gctgcgactg cgaaattcgc
     1681 ttgaccgggt agtgatcgcg cgcaaaggtg gcgaacagtt cgccctggcg caccacatca
     1741 tagtgcatgg ggatcacgat gggcggtttc acctgctcga tcactttaat gatgtcctca
     1801 aacgccaaat tggtcatggc gtcgatggga atcatcatca cgtccacatg ctgcaacacc
     1861 ttcacctgtt ccgcagtcag gagatggcca atattgccca aatgcgcgag acacaagctc
     1921 cccatctcgt agatgaaaat ggagttggca atggcccccc aactggcact ccgttgactc
     1981 ggaatattga cgatggtgat gtccttgatg ctggtccgga tcgggttcca tgtctgtttg
     2041 aaggtaatcc cacgcaagat caccggatct cctgtcacag cgccagtatt gttatgggta
     2101 aaatgatcgt tgctgacggt caccgcgtcg gcctggattc ccggcgtcac gttgaaattc
     2161 ggatcggcaa ccacttttgt atggctgccg gactggatta aaaaacttga gtgtccaaac
     2221 caattgacga aatacgagcc aagagatgga gccttcgctc cgttacctcg agcctgcacc
     2281 aagtgtcgcc catgatgaaa cagctcgacc aaagaaggat tgcacatcgc ttcggcgaca
     2341 ccgcgcagcc acagtaacca agcgctccca acgacaccga gaaacccgcg tcgtttcatt
     2401 tttcctcctc gatctctaag gacgtgaata gagaaacttg ggtccccggc cagcgccagg
     2461 cattgctcgg caaatttgct ttacgacagg tctgctccaa aaacgccgat ccatcccagc
     2521 cgtgctctac cgcgacctga ggtaagagca cgccccgttt gccatggcgc gcgatgtaaa
     2581 tcccatgctt cccgatctcc agatggagcg ggtggtcagc cggatcgagc ggtgaaatga
     2641 cggaaatatg gatctgaata tccttgagct catcgacgca cacaggttta actcgacgat
     2701 cgcatgtcgc ggcagcttcg gtcatttcga ttacaatgtt gaaaagcccg ctggatggcg
     2761 cacacgttcc aacacagccg cgcagaagat ggtgattgaa caacgttaca aatgcgccgt
     2821 agttgataca atcgaggtgg ggatcgctct caccgggctc gccgtcctga tttccgcgag
     2881 taatcgcttc gagggtttgc cgggcggttt tgatcagccg ccgttgcgag tcgaaaggaa
     2941 gatagacatc gggcatgacc gcaccgagtt gggtcttcga atctagctcc cgctataagg
     3001 gaatacaact gccggtgaac gcgacgacta cttcgagcaa tgtttgcaga tcccgtaaaa
     3061 agcgagttgg tgcgaggtca ctctaaatcc ttcatgaggc agcttgacag gttcgatttc
     3121 cctttgattc tggagcacga catcatagac ccgatcgcat atttcacaaa taaaatgttg
     3181 atgcttctgt accagcggat cgaaatgctg cgagcggccg cgcatcaaga cctgcagctt
     3241 ctgatcggca accaactttt gcaagtttcg gtaaactgtc ccgagactga tgctcggtag
     3301 tttttttcgc acgcgatcat aaatttgatc cgcggtgggg tgagatttgt cactcttgat
     3361 cgtctcccag atcgtgccga gctgctttgt ggttcgcgcc tgttttctgg tttttgccat
     3421 attaactagc aagatcttcg gccaatgatt gctgcgcttc gggagcaaga attgtgcgcg
     3481 cttcgtaatt gggatgatgg ataatcaact cggcgcgttc gccggcaata aaagcccggc
     3541 gcgcctcctc atcgaagcaa aaacgcacat actgcacggc gctgaccttg tctgctttac
     3601 tgcgcccaga ttcgaatctc gccggtagcg atcggccgct gatgctcaag gacacacatt
     3661 cgtcgatacc gaggaatttg agcaggtcat cgcgcagatg actttgatcc tcgagttcca
     3721 aaaagagcgt ggcgctgagc tccccagagt ttggaatgag ttcgttgtaa acagcgactt
     3781 cctcccggat cttgtccagg tccgatattt tttccgcgcg caacatctcc tgaatttgaa
     3841 aaatcaccgt gtcccgattt tcaaaaacta atgacacttt agcgccgaca gcgagccggc
     3901 ggcttttttt cttttcgata atgtctttgc gaaattcctc gcgcaccttc tcataggcaa
     3961 ccaatcctag aatatcgtcg agcactatct ttttcatgtt tctgactatt cagtgtttca
     4021 atcagcaaaa actttttcaa ccgttgttaa cccgcaggag gaagggccat acccggccct
     4081 ccctccgatc gaatccgctt ctgctcaact gcccagcgta tcgagtcctt tgctgaacct
     4141 gccagcatgg gatttctccg ccttggctag agtttcaaac cattcagcca gttctggcaa
     4201 tccttcctcg cgcgctgttt tggcgaaccc cggatacatt tccgtgtact cgtaggtttc
     4261 gccttcgacc gctgatttga gattttttgc cgtggtgccg atcgcgaccc ccgtgcaggg
     4321 atcacccacc tcttttaaaa aatcgagatg tccgaaggcg tgcccggtct cggcttccga
     4381 cgtgtcgcgg aagagaccac ccacatcggg gtagccttcg atatccgcaa cccgtgcaaa
     4441 atatagataa cgtcgattcg cctgagactc gcccgcgaag gcatgcttca ggttttcgtg
     4501 actctttgtg cccgccaagc tttttcccat aaaccctcct taatagtaat gattactgat
     4561 aatacgaagc ccttgaggaa gtcaaagagg ctgttcgctt tttttaattc gccggttctg
     4621 atatttttgc tatgatgact cgcaggactc gatgatctcg atcgaagact ttcgcaaagt
     4681 cgaactcaga gttgccatta tccaat
//
LOCUS       DASULZ010000440         4688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_764689, whole genome shotgun sequence.
ACCESSION   DASULZ010000440 DASULZ010000000
VERSION     DASULZ010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764689"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VFY96_08955"
     CDS             complement(<1..235)
                     /locus_tag="VFY96_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606629.1"
                     /translation="MKKIVIQSLILFAVLLGAWQSAYAQSKPLEKVRLTVPAKALTFV
                     PYYFGKNQGIFTKEGIDLEIIVMRPPLGVTALLA"
     gene            complement(370..1788)
                     /locus_tag="VFY96_08960"
     CDS             complement(370..1788)
                     /locus_tag="VFY96_08960"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX5606630.1"
                     /translation="MHYHDLRGWLKYVDSFGELRVIEGADWNQEIGAAAEVAAQLDPP
                     PAVLFDKIKGYPAGRRVLTGIHNPTLKRQCLTNHLPLDYSRDQFIEGWKKRLDHPKLI
                     PPRAVDSGPLLENIFEGQDIDLLSLPAPHWHEGDGGRYLGSLDITISRDPDEAWINLG
                     CYRVMIHDRDTLALYISPGHHGNIHRQKYFDRGKPFPVAISFGPDPLLWFFGQMEIPA
                     GQSEYDYAGGVRGEAYEVICGKHTGLPIPAYSEVAIEGEVIPGTSIPEGPFGEFTGYY
                     AGGLRSEPMLKVKRLMYRNDPIITGAPPFKPAAGAENNLIRAAYIWHYLDKTGVPDVR
                     GVAYYQTRMLVVVAIKQRYPGHARQAALIASQCRAAALLTRYIVVVDDDIDIWDSNDL
                     LWALCTRTDPAKDIDILRRCWSNPIDPIIPPSERGFQSRGIIDACKPYEWLSDFPKVS
                     GASEALKKKVTTKFGASLGGKN"
     gene            2019..3215
                     /locus_tag="VFY96_08965"
     CDS             2019..3215
                     /locus_tag="VFY96_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008464244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEX5606631.1"
                     /translation="MAYAPGTGPVDKLSLPAPFATPSARNTSKVVGWPKGKMPTAAPG
                     FEVSLFADNFDNPRQAYVLPNGDILIVEATREWIGRADRPEKSANRITLLRDTNGDGK
                     PDLRETFLTGLNMPHGMMALGNWFYVGNTNGVVRYPYRTGQTRIEGKGEKILDLPTDG
                     HYTRDLIADPAGKKIYVAVGSASNVDEENTWEKDQRRAGILEINPDGSGMRIFANGLR
                     NPVGMDWEPQTKTLWTVVNERDLLGDDLVPDYLTSVKEGAFYGWPFSYFGQIEDPRKK
                     GQRPDWVAKAIKPDYALGSHVAALGLAFYNGKTFPERYRGGAFIGMHGSWNRSKMVGF
                     KVAFVPFANGKPAGPMEDILTGFIADEGKFEAHGRPVGVTVAPDGSLLVADDSGGKVC
                     RVTAKR"
     gene            3303..4556
                     /locus_tag="VFY96_08970"
     CDS             3303..4556
                     /locus_tag="VFY96_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009285627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEX5606632.1"
                     /translation="MSHRYLLFAVSVCASLLFWFSIDAAEQNNSHVVETGPAEKLILP
                     PPNSTRAVANPSTVVPWPKGQTPKAPAGFEVTLFADDLDNPRMIYVLPNSDVLVMESM
                     PQQSQSRIVLLRDSAKRGRPDLRQTFLRRLNMAFGMTLIGNRFYVGNTDGIVVLPYAT
                     GETRLQRRPEKILDLPSGGHYTRNLLADPAGKKIYVAVGSATNVDEQRVDEKDPRRAA
                     ILQVNPDGSGMRVFASGMRNPVGMDWEVKTNRLWTVVNERDMLGDELVPDYLTSVRDG
                     AFYGWPYSYFGQNEDPRKKAQRPDLVAKAIKPDYALGSHVAPLGLAFYNGKTFPEHYH
                     GGAFIGMHGSWNRSNLVGYKVAFVPFENGRPSGAIEDFLTGFIVNPKSREVYGRPVGV
                     ALWTDGSLLVADDAAGKIWRISARN"
BASE COUNT          999 a         1354 c         1310 g         1025 t
ORIGIN      
        1 cggccaagag cgccgtcacg cccaacggcg gccgcatcac gataatctcg agatcgatcc
       61 cttctttggt aaagattccc tgatttttgc cgaagtagta gggcacaaac gtcagcgcct
      121 tcgccggaac cgtcaggcgc actttttcaa gcggctttga ctgcgcatac gcgctttgcc
      181 atgcgccgag caaaacagcg aacagtatta aactttggat gactattttt ttcatctaat
      241 cctcgtgtct ccccgcctct tttcctctcc gcatcgtcgg gatgaaggtg ggggcgcttc
      301 tcgcgtttca tcaaaaatgg tgaccgccgg tcgcccgaca cactcctccc tgttctctgc
      361 gtctctgtgt taatttttgc cgcccaagct ggcgccgaat ttcgttgtca cttttttctt
      421 cagcgcttcg ctggcccctg aaacttttgg aaaatcgctc agccattcat agggcttgca
      481 agcatcgatg atgccgcgcg actgaaaacc gcgctcgctc ggcggaatga tcggatcgat
      541 gggattgctc cagcagcgcc tgagaatatc gatatcttta gccgggtccg tgcgcgtgca
      601 cagcgcccag agcaaatcat tgctgtccca aatatcaata tcgtcgtcga ccacgacgat
      661 gtatcgggtc agcagcgccg cggcccggca ttgggatgct atcagcgccg cttgccgcgc
      721 gtgtccggga tagcgctgct tgatcgccac gacgacgagc atacgcgtct ggtaatatgc
      781 gacaccgcgg acatccggca cgcccgtttt gtcgagatag tgccagatat atgcggcgcg
      841 aatcagattg ttttccgctc ccgccgcagg cttgaacggc ggcgcaccgg tgataattgg
      901 atcgttgcgg tacatgagcc gcttgacttt gagcatgggt tcggagcgca atccgccggc
      961 atagtacccg gtgaactcgc cgaacggacc ttcaggaatg ctggttcccg gaataacttc
     1021 accttcaatc gccacctcgg aataagctgg aatcggcagg ccggtgtgct tgccgcagat
     1081 gacttcgtat gcctcgccgc gcacgccgcc ggcataatcg tactccgact gccccgcggg
     1141 aatttccatc tggccaaaaa accaaagtaa cggatcggga ccgaagctga tggccaccgg
     1201 aaacggcttg ccgcgatcga aatacttctg ccggtgaata ttgccgtgat gaccgggcga
     1261 gatatagagt gccaaggtgt cgcggtcgtg aatcatcacg cgataacagc ccagattgat
     1321 ccaggcttcg tctggatctc ggctgatcgt aatatctaaa gaaccgaggt agcggccgcc
     1381 gtcgccttca tgccagtgcg gcgccggcaa tgacaacaga tcgatgtctt ggccttcgaa
     1441 aatattttcg agcaacggtc cggagtcgac cgcccgaggc ggaatgagct ttggatggtc
     1501 gaggcgtttt ttccagccct cgataaattg atcacggcta tagtcgagcg gcaaatggtt
     1561 ggtcaggcat tggcgtttga gagttgggtt atgaattccg gtgagcacac gacgccccgc
     1621 tggatagccc ttgatcttgt cgaacagcac cgccggcggg ggatccaatt gagccgcaac
     1681 ttccgccgct gcgccgatct cctgattcca gtcggctcct tcgatcacac ggagctcgcc
     1741 gaacgaatct acgtatttta gccaaccgcg caagtcatga taatgcatcg ccgatcctcc
     1801 agtgcgcgga tattagaacg gagaagccgg ctgatacaaa cggcgctttg cgcattttcc
     1861 gctggacaaa actaaggtat attgcaccac cgtctcatcg gccgaactat tttgtgaggg
     1921 aggtaagcac gatggttaac tgtctgtccg aacgcgcggg actcgcgctg gctattctgg
     1981 tttctgcttg cgcgtcagat caaattcaac gatccgaaat ggcttacgcg cccggcaccg
     2041 gaccggtcga caagctttcc ctgccggcgc catttgccac tccctccgca cgcaatactt
     2101 cgaaggtggt cggttggcca aaaggtaaga tgccgactgc ggcgccggga ttcgaagtca
     2161 gcttgtttgc cgacaatttt gataacccgc gccaagccta cgttttgccc aatggcgata
     2221 ttttaatcgt cgaggcgacg cgcgagtgga tcgggcgcgc ggatcggccg gaaaaatcgg
     2281 cgaatcgaat caccctgctt cgcgatacca acggcgatgg caaacctgat ctgcgtgaga
     2341 cattcctcac cggattgaat atgccgcacg gcatgatggc attgggcaac tggttctatg
     2401 tgggcaacac caatggcgtc gttcgttatc cctaccgcac cgggcaaacc agaattgaag
     2461 ggaaaggcga aaaaattctc gaccttccga cggacggcca ctacacccgc gacctgatcg
     2521 ccgatcccgc gggcaagaag atttacgttg ccgtcggctc cgcgtcgaac gtcgacgaag
     2581 aaaatacttg ggaaaaagat cagcgccgtg cgggaattct ggagatcaat ccggacggca
     2641 gcggcatgcg aatttttgcc aacggactgc gcaaccccgt cggtatggat tgggaaccgc
     2701 aaaccaaaac cctctggacc gtggtcaacg agcgcgatct gctcggcgac gatctcgttc
     2761 cggattatct aacgagcgtg aaagagggag ctttttacgg ttggcccttt tcctatttcg
     2821 gtcagatcga ggatccgcgc aagaaaggcc agcggcccga ttgggtcgcc aaagcgatca
     2881 agcctgacta tgctttggga tcccatgtcg cagcgctcgg tctggctttt tacaacggca
     2941 aaacatttcc cgaacgatac cgtggtggcg cgtttattgg catgcacgga tcctggaatc
     3001 gctccaaaat ggtcggcttt aaagtcgctt tcgtcccgtt tgcgaatggt aaacctgccg
     3061 gtcccatgga agacatcctg accggcttca ttgccgacga gggtaagttc gaagctcacg
     3121 gccgcccggt cggagtgacc gtcgcacccg acggttccct tctggtcgcc gacgacagcg
     3181 gcgggaaagt ctgccgcgtt acggccaagc gataggcaaa aattaaccgc agagagacgg
     3241 cgggcgcaga gatttttctc gaaattcctg cgcgcctccg tggggccatc cgatcttctc
     3301 acatgagcca tcgttatcta ctcttcgccg tctcggtttg tgcttcattg cttttctggt
     3361 tttcgattga cgctgccgaa caaaataatt cccatgtggt cgaaactggc ccggcggaaa
     3421 aattgatcct gccgccgccc aactccactc gcgcggtggc caatccctct actgtggtgc
     3481 catggcccaa ggggcagacc ccgaaggcgc ccgccggttt tgaagttact ctgtttgccg
     3541 atgacctgga caatccacga atgatttacg tgctaccgaa cagcgatgtg ttggtgatgg
     3601 aatcgatgcc gcagcagtcg caaagccgga ttgttttgct gcgcgacagc gctaaacggg
     3661 gccgacccga tctgcgccaa acctttctgc gccgcctgaa catggccttt ggaatgacat
     3721 tgatcggcaa tcgtttttac gtcggcaaca ccgacgggat tgtcgttttg ccctatgcga
     3781 ccggcgagac ccgtcttcaa cgccgtccgg aaaaaatcct cgatctgccc agcggcgggc
     3841 attataccag gaatttgctc gccgatccgg ccggaaaaaa gatttacgtc gccgtcggct
     3901 cggccaccaa cgtcgatgag caacgggtcg atgaaaaaga tccccgccgc gcggcgattc
     3961 tacaggtcaa tccggacggc agcggcatgc gcgtgttcgc gagcggcatg cgcaatcctg
     4021 tgggcatgga ttgggaagtg aaaaccaatc gtttgtggac cgtggtcaac gagcgcgaca
     4081 tgctcggcga tgagctcgtg cccgattatc tcacgagcgt tcgggatggc gctttttacg
     4141 gctggcccta ttcgtatttc ggccaaaacg aagatccgcg caaaaaggcg caacgtccgg
     4201 atctcgtcgc caaggcgatc aagccggatt acgctttggg ttcccacgtc gccccgctcg
     4261 gtctcgcctt ctacaacggc aagacttttc ccgagcatta tcacggcggc gccttcatcg
     4321 ggatgcatgg ttcttggaac cgctcgaact tggttggcta caaagttgcg tttgtgccct
     4381 tcgaaaacgg cagacccagc ggcgcaatcg aagattttct cacaggcttt atcgtcaacc
     4441 ccaaaagccg ggaggtttac gggcgacccg tgggcgtggc gctatggacg gatggctcgc
     4501 tgctggtcgc cgacgacgcg gccggcaaga tctggcggat cagcgctcgc aactaaatgg
     4561 gcagtcgttc ggctccgagc tttcgatgtc gcggagcgga tcgccacgat aacgcgaaga
     4621 agcttcactc tgagaatgcc tagactcgac cgacttctaa caattggccg gaaacgtaat
     4681 ccgaatcc
//
LOCUS       DASULZ010000441         2379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1374686, whole genome shotgun sequence.
ACCESSION   DASULZ010000441 DASULZ010000000
VERSION     DASULZ010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374686"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VFY96_08975"
     CDS             <1..876
                     /locus_tag="VFY96_08975"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX5606633.1"
                     /translation="FAGMFEGQYDVSEMSLGELIYYTSRARADFVAIPIFPSRMFRHG
                     FIFCRKSSGVHQPSDLNGKRIGFLRWVQTAAIWMRGMLIDQYGVSPRDTEWYVASMHH
                     WDDDDPGATVDPRDGSTVRMLQNRGTTTSERACRALFDGQVDALGVTESQLPLLLADN
                     SVRRLFESPREVEADYFRRTKILPIMHVVALQKRVVESHPDLPERLFRLYSEAKRWAQ
                     RWRRAIPSLVEAWPNHYFAEEQEIFQTDPWVYGLEANRHVLETFLGYCHAQGVTARTI
                     AAEDIFHPSTLKLVE"
     gene            895..>2379
                     /locus_tag="VFY96_08980"
     CDS             895..>2379
                     /locus_tag="VFY96_08980"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX5606634.1"
                     /translation="MATASAAAINSTSLSCDLRDWLKEVKRLEQLEQISGAHWDLEIG
                     ALTEIILERIATPPALIFDNVPGFDPKRRVLANMLETLERTALTLNLPLDMKTIPLID
                     ALRARLRAMQPLKANVVSSGPVFENIERGEEVDLTKFPVPRWHAGDGGRYLGTAHLVV
                     TRDPETGVENVGCYRVMLHDRDKVGLYISPGKHGKIHYEKAMKAGKPFPVAMAFGQHP
                     LLYIAASQAVPYGINEYDWAGGLIGQPIDVIKGPVTGLHFPASAEIVIEGEIIPGESL
                     QEGPFGEWPGYYASARRAEPFVRVKALYHRNEPIICGAAPFKPTIHGMYRSCLRAAMV
                     WNGMEQAGVPDIKGVYLPPPAQRFMIVVAIKQRYPGHAKQAALIACQCHAGAYLGRYV
                     VVVDDDIDITDVNEVLWAVATRSDPATSVDILRRTWSGPLDPIIPPGQKGHNSRMIIE
                     AVRPWEWRDKFPATSAIDDETRTQYSKKWQKQLLQVQARHSRGRS"
BASE COUNT          499 a          661 c          717 g          502 t
ORIGIN      
        1 ttcgccggta tgttcgaagg acagtacgac gtctccgaaa tgtccctggg cgaactcatc
       61 tattacacct ccagagccag ggcggatttt gtcgctattc cgatttttcc gtcgcggatg
      121 tttcgccacg ggttcatttt ctgccgcaag tcctccggcg tccatcagcc tagcgatttg
      181 aacgggaaaa gaattggttt tctgcgctgg gtgcaaaccg cggcgatctg gatgcgcggc
      241 atgttgattg accagtacgg cgtgtccccg cgggacaccg aatggtatgt ggcctccatg
      301 caccattggg acgatgacga cccgggcgcc acggtggacc cgcgcgacgg ttcgaccgtt
      361 cgcatgctgc aaaaccgcgg tacaaccacc tccgaacgag cttgccgcgc gcttttcgac
      421 ggtcaagttg atgcgttggg agttacggag tcgcagcttc cccttctgct tgcggacaat
      481 tcggttagac ggctgttcga aagcccgcgc gaggttgagg ccgactattt tcgtcgaacc
      541 aaaatcttgc cgatcatgca tgttgtggcg ctgcagaaac gggttgtcga gagccatccc
      601 gacctcccag agaggctttt ccggctctac agcgaggcaa agcgctgggc gcagcgctgg
      661 cggcgggcga ttccaagcct ggtcgaagct tggccgaacc actatttcgc cgaggaacag
      721 gaaatttttc aaaccgatcc ttgggtttat ggcctagaag ctaaccggca tgtgctcgag
      781 acgtttttgg gctattgtca tgctcaagga gtcactgcaa gaaccatcgc ggcagaagat
      841 atctttcatc cgagcacgct gaaattggtc gagtaaccgt ttaggaggag tcccatggcg
      901 acggcgagcg cagcggcaat caattccacc tctctttctt gcgatttacg cgactggtta
      961 aaagaggtca agcggctcga acagctcgag caaatctcgg gggcccattg ggatctggaa
     1021 attggcgcgc tgacggagat tattttagaa agaatcgcta ctccgccggc gctgatcttc
     1081 gataacgtcc ccggtttcga tccgaagcgg cgcgtactcg caaatatgct cgagaccctc
     1141 gagcgtaccg cgctcaccct gaatctgccc ctcgacatga agacgatccc tttgatcgat
     1201 gcgttgcgcg cgcggctgcg agcgatgcag ccgctcaaag ccaatgttgt ttctagcggt
     1261 cccgtcttcg agaacatcga gcgtggagag gaggttgacc tcacgaaatt tcccgtgccg
     1321 cgctggcacg ccggtgacgg cggcagatat ctcggcacgg cgcatttggt cgtgacgcgc
     1381 gatcctgaaa cgggagtcga gaatgtcggc tgctatcggg tcatgctcca cgacagggat
     1441 aaagtcggac tgtacatttc gccgggaaag cacggcaaaa ttcattacga gaaagcgatg
     1501 aaagccggca agccgtttcc ggtcgcaatg gccttcggac agcatccgct tttgtacatc
     1561 gccgcatccc aggcggtacc ctatggaatc aatgagtatg actgggcggg cggcctgatc
     1621 ggccagccca tcgacgtgat taaaggtccg gtgaccgggc tgcattttcc cgcctccgcc
     1681 gaaattgtga tcgagggcga gatcattccc ggggaatcgc tgcaggaagg tccgttcggc
     1741 gaatggccgg ggtattacgc gagcgcgcga cgggcggagc cgtttgtcag agtgaaagcg
     1801 ctctatcatc ggaacgagcc gatcatctgc ggcgctgcgc cattcaagcc aacgattcac
     1861 ggcatgtatc gttcctgctt acgcgcggcg atggtatgga atggcatgga gcaggcgggg
     1921 gtgccggata tcaagggagt ctacctgccc ccgcccgcac agcgtttcat gatcgtggtg
     1981 gcgattaagc aaagataccc cgggcacgcg aaacaagccg ctctgatcgc ttgccagtgc
     2041 cacgctgggg cttatctggg acgctatgta gtagtggtcg acgatgacat cgatatcacc
     2101 gacgtgaacg aggtgctttg ggccgtcgcc acgcgttcgg atccggcgac ctccgttgat
     2161 atcctgcggc gtacatggag cggacctctt gatccgatca ttccccctgg tcagaaggga
     2221 cacaactcgc gcatgattat cgaagccgtg cgaccatggg agtggcgcga taagttcccg
     2281 gcgacgagtg ccatagatga tgagacgcga acgcagtact caaaaaagtg gcagaagcag
     2341 ttgctgcaag ttcaagctcg ccatagccga gggcgctcg
//
LOCUS       DASULZ010000442         2009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_663765, whole genome shotgun sequence.
ACCESSION   DASULZ010000442 DASULZ010000000
VERSION     DASULZ010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663765"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1419..>2009)
                     /locus_tag="VFY96_08985"
     CDS             complement(1419..>2009)
                     /locus_tag="VFY96_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013925392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5606635.1"
                     /translation="CSGLVLFAHGSGSSRHSPRNNFVAQVLQDLGLGTLLLDLLTREE
                     DASYENRFDIDLLTWRLERATQWVMEQPRSESLDLGYFGASTGAAAALQAAATFGNSI
                     GAVVSRGGRPDLAMTALGNVEAPTLLIVGGLDHEVIELNRRAYQKLKAEKHLEVVPGA
                     THLFEEPGALQEVAGLAGQWFKRHLGRKRAQEPRRN"
BASE COUNT          473 a          549 c          524 g          463 t
ORIGIN      
        1 aatcatcgca ggtttgttga tcatgaggcc gatcataaag aacgaaaaag tccaacccat
       61 ttggccacag caaacagcgt tgatcgaaat gccagatcgg actgcctatt ttgcgtcggt
      121 attggattgc catgattgca agccccctat taggttttat cgcccggctc cactcaaagc
      181 tatagggcaa cgaaacgtaa aatgaccgat accgggctgg accttcatgc caaaattcaa
      241 cctatctggt cttgctgtcc agttcggagc ccggtccggc gggcaataag actggcgctg
      301 ccatcccatt ctagtgtaac tgcgcaactc cacaatttta agagagcgca atcaccgtgc
      361 cggtaaacta gctctttgtg cttgccggat tgtcgcgagt gcgaaaaact cactggtttc
      421 ttttcagcca taggaaagcg agacgcatta cggtgatact ttctcagcga aatatttgtt
      481 ttttccctcg catcacgcgg cccattccct gttcggcttt tcgttgggga accagtcgca
      541 cacggcatca gatttcgacc atttgatcac gcagaaacgg cacgacagct aagataattg
      601 ctttttatct tcaatccttt ttcttggcat gggtcatgcg cttgtacaga aggggatgag
      661 aaagaattcg aggaaggatg caaatactat gacccgcaat tggaatggct acctggttag
      721 ggccctcaat gagtttgcgt cacttgctgc tgcgttccgg gacacggatc cgagccatcg
      781 gattccggcc ccagcgaaag cttcgatagg tcataaacga agccaaaaga aatcgtcggg
      841 cacaccaagg aaacgaaaac cccatcgtac tgagtgctgc ctcgattgca acggataact
      901 ttgactacga cggctttgcg ttgcgccgat ttatttctga agccgagagc gtgcgcgcag
      961 ccctaatgcg acttaaaaaa gcatttgccg caaacccctt gaaccagccg cctttagaaa
     1021 tacaatggtg gtctcacgaa ggcccaaaga atcgccagat tcgcagttgt ttctgaaacc
     1081 tgcggagggc gacccgcatc tgtccagact gcatcaagct agaagccgcc gacgattagc
     1141 aacacactga ttattttatc accggcgatt ttttggacat cgtgttggtc cgcagcgtct
     1201 cgtagcggtc cgcttcactt gtctagctca gtcgtgccat tggttcgggc tcggtcgggg
     1261 tacgggttca ctgcggcgtt tcatacaggt tgaagaaagt agcgggggca tagcaccttg
     1321 agcgcagtat ccgaaaacgg cgaggagcac agaggatcgt cgatgaagtc gctgaggcgg
     1381 agcggtgtaa ggacatgcct tgctcacgca gcgggtgctc agttacggcg cggttcttga
     1441 gcccgttttc tgccaaggtg tcgtttaaac cattgcccag ccagaccagc cacttcttgc
     1501 aacgcgcctg gctcctcaaa caaatgggtt gcgccgggga cgacctccag atgtttctca
     1561 gctttgagct tttggtaagc gcgccgattg agttctatga cctcgtgatc taagccgccg
     1621 acaatcagca aagtcggcgc ttcgacgttg ccaagcgctg tcatagccaa atcaggacga
     1681 ccgccgcgcg ataccacagc accaatggag ttaccgaacg tcgcggccgc ctgcagcgct
     1741 gccgccgcac ccgtgctcgc tccgaagtaa ccgaggtcga gagattcgct ccgtggctgc
     1801 tccatcaccc attgcgtcgc acgttccagg cgccaggtaa gaagatcgat atcgaaccgg
     1861 ttctcgtaac tcgcgtcttc ctctcgggtt aaaaggtcga gcagtaatgt tcccaatccc
     1921 aagtcttgca gcacctgagc tacgaaattg ttacgtgggc tatggcggct gctgccactg
     1981 ccgtgggcga agagcacaag accgctaca
//
LOCUS       DASULZ010000443         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_461032, whole genome shotgun sequence.
ACCESSION   DASULZ010000443 DASULZ010000000
VERSION     DASULZ010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461032"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VFY96_08990"
     CDS             <1..579
                     /locus_tag="VFY96_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606636.1"
                     /translation="VLAGDPGDSNLDLIRKKILRTHGIDESDYQIEIIGSSPVRLQAF
                     LDRRVVAAMLTPPASDKALASGGVLLANSEAYVPGWLLTCGWGLRRWLAEHRAIVVRF
                     IRAWSAATDWLLKPENRDETIDLVMREEKLSRAAATDAYHKVVPKARINPAALCTVIE
                     LRKEMGVYQPPYAPPEQFYDASYWNEATAGGK"
     gene            complement(634..1497)
                     /locus_tag="VFY96_08995"
     CDS             complement(634..1497)
                     /locus_tag="VFY96_08995"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5606637.1"
                     /translation="MSDIENVKPVDKFVSVDGLKLRYIEAGSGPSVLFLHGASLGSSA
                     DVFLRNLGPFAKAGFRAVAFDYPGFGLSDVPAKQSTAQQRNSIPKFIDAAGLGKTALI
                     AHSRSGGFAVQLALEDPSRFSHVIVLGTGSLLPPQTQEQVGKYEAVQARVDREMAQKE
                     PTLEDARKLLQADTFNHALILDDDVALRHRSMIGRNFKAHQDRQNQEAPAGGGASQAK
                     PLWQRLTELKMPLLMIFGREDRAHAGERAELLKNMHPELNIHIVNGCKHMVHWDAFDD
                     LMRLGVPFLKS"
BASE COUNT          445 a          562 c          556 g          447 t
ORIGIN      
        1 gttctggcag gcgatccagg cgattccaat cttgacctga tccggaaaaa aattctgcgc
       61 acccacggaa tcgacgagag cgattatcaa atcgagatca tcggcagctc gccagtgcga
      121 ttacaggcat ttctcgatcg ccgtgtcgtc gctgcgatgc taacccctcc cgcttcagac
      181 aaggcacttg cgtcgggagg cgtcttgctc gccaactcgg aagcgtatgt ccccggttgg
      241 ctgctcacct gcggctgggg attgcgccgg tggctcgcgg agcatcgcgc aatcgtggtg
      301 cgttttattc gtgcctggtc agcggcgacc gactggctgt taaaacctga aaaccgcgat
      361 gaaacgatcg atctggtcat gcgcgaagaa aagctctcgc gcgctgccgc gaccgacgct
      421 tatcacaagg tcgtgcccaa ggcgcgaatc aatccagccg cgctgtgcac ggtcatcgag
      481 ctgcgcaagg aaatgggagt ttaccagccg ccctacgcac caccggaaca attttacgac
      541 gcgagttact ggaacgaggc gacggctggc ggaaaatgaa gtagtcgttc cgcagttacc
      601 cttgtctatc ggcgtcgatt gaaaaatcgc tcgctagctc ttcaaaaatg ggacgccgag
      661 acgcatcaga tcgtcgaacg catcccagtg caccatgtgt ttgcagccat tcacaatgtg
      721 aatgttgagt tccggatgca tgttcttgag caattcggct cgctcgcctg catgggcgcg
      781 gtcttcacgg ccgaagatca tcaacagcgg catcttcaat tcggtgagcc tttgccacaa
      841 gggtttggcc tgtgaagcgc cgccacccgc cggtgcttcc tgattctgcc gatcctgatg
      901 cgctttgaaa ttccggccga tcatacttct atgccgtagg gccacgtcgt cgtccaagat
      961 caaggcatgg ttaaacgtat cggcctggag gagtttgcgg gcgtcttcca gcgtcggttc
     1021 cttttgcgcc atctccctgt cgacgcgcgc ctgcaccgct tcgtacttcc cgacctgctc
     1081 ttgggtctgc ggcggcagca gcgatccggt gccgagaacg atcacatgcg aaaagcggct
     1141 gggatcttcc aaggcgagct gaaccgcaaa cccgccggag cgtgagtggg cgatcagcgc
     1201 ggttttgccg aggcccgcgg catcgatgaa tttcggaatg gaattgcgtt gctgtgccgt
     1261 gctttgtttg gcgggaacgt cggagaggcc aaaaccggga tagtcgaagg cgaccgcgcg
     1321 aaacccagct ttggcaaacg ggcccagatt gcgcagaaat acatctgccg agctgccgag
     1381 cgatgcaccg tgcagaaaca acaccgacgg tcctgagccc gcttcgatat aacgaagctt
     1441 gagtccatcg acgctcacga acttgtctac cggttttaca ttttcaatgt cgctcatcgc
     1501 aaattctcct tctcaaccag attggattag cttggctgaa gctgctgccc catgcaattg
     1561 tttatggaag tttgtagaac caagttcttt tgacggagct ataacgccga accgccgagc
     1621 tgtcaatcat ggcactgggt aaacgaatta ggcctttgta acgccagtaa cgccgcttgc
     1681 tgcggaagga ttctttttcc actctcgacc tcacagaaca actgccaaat acgcaaatgg
     1741 ttcgcattgg agcgtggcgg ttgtcccgag tacaacgccg ggaatgatgc cgcttgacag
     1801 aaatgcttct tagtcataaa cctccagcga aactacgcga gctgggtctt tagatccggt
     1861 catctcttcg aatgatttct atagcccgga ctggagttat tcagccgggc tattttgttt
     1921 ctggaacgag atccgcaact gcagcaatga gcgagcaaag tgaatcacga aaagctcaca
     1981 gaagtggaaa acaggtcccg attgggcaac
//
LOCUS       DASULZ010000444         2857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_562691, whole genome shotgun sequence.
ACCESSION   DASULZ010000444 DASULZ010000000
VERSION     DASULZ010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562691"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VFY96_09000"
     CDS             complement(<1..668)
                     /locus_tag="VFY96_09000"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5606638.1"
                     /translation="MKRFLYELTESWKIAAAQMRSNKTRSTLTALGVIIGIVAVTLMG
                     TAISGIQVGFDKSMAIFGDDVLYVSQWPWKPVDDWWNFRDRKKIKTEYAEPINRIIAA
                     TPNSNLVIAVPTSSLLRSVKYRQSEVSNVLIRGTVSDYLITSTFDFKEGRFFNDVESR
                     GGANVCVIGYDVADALFPSESPIEKPVLINGQPFRVVGVISRQGTFLGLFSLDSIVVM
                     PLPA"
     gene            complement(668..1366)
                     /locus_tag="VFY96_09005"
     CDS             complement(668..1366)
                     /locus_tag="VFY96_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5606639.1"
                     /translation="MGEETVHALRGVSLQIRSNEYLAIMGPSGSGKSTLMNMLGCLDT
                     PSSGRYEFNGRDVSAMSDDELAAIRNREIGFVFQTFNLLPRADSLRNVELPLVYAGID
                     PETRRERAAHVLREVGLGDRLHHKPNELSGGQRQRVAIARALVNDPSIILADEPTGNL
                     DSKTGDEIMTLFENLYQRGHTIILVTHEADIANHARRTVRLRDGLIESDDGGFDGIKN
                     LPVEDPLPADAAVY"
     gene            complement(1432..2793)
                     /locus_tag="VFY96_09010"
     CDS             complement(1432..2793)
                     /locus_tag="VFY96_09010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5606640.1"
                     /translation="MHQALTKPAVARETAKKRRQGRRRKILFGSAGAVLLLIIALVVA
                     SKREKPIPVTTEKAVRRTILQTVSATGKVQPETEVKISPEVAGEITELPVEDGMQVKK
                     GDLLLKIKPDSYKALLEQQEAAISSAKATNLQQKATMLKTEHDFRRSKDLFEKKLISE
                     QEYNAAEAANDVAKNTYESSLHEIERAEASSSQARDQLSKTTIYAPMDGTVTILNSKL
                     GERLVATNQFAGTDVMRVADLTHMQAVVDVNENDVVNVKLGDKANIKIDAYGDRKFKG
                     VVQQIANTGKTTGTGTQEEVTNFEVKIRVDDHDVALRPGLSCTADIETNMVKDAVAVP
                     MQSVTIRTGDSNLSPEEIEKQKQKVAARDKDDNKAELSNDRVEKQTQKEERERLVKVV
                     FLKNSNKAQMVKVTTGIADDSYMEIKSGIKPGDEVISGSYSAISRKLKEGAKIAYDKE
                     VAK"
BASE COUNT          530 a          798 c          759 g          770 t
ORIGIN      
        1 aatgccggca atggcatcac cacaatcgaa tccagactga ataatcctaa aaaggtgcct
       61 tgccgtgaga tgaccccgac taccctgaaa ggctgcccat tgatcagcac tggtttctcg
      121 atcgggctct cggaagggaa cagcgcgtcg gctacatcgt agccgattac acatacgttg
      181 gcaccgcccc gggattccac gtcgttaaaa aatcggccct ctttaaaatc gaaagtggaa
      241 gtgattaggt aatccgagac agtgcctctg atcagtacgt tactgacttc gctttgccgg
      301 tatttgacgc tgcgcagcag actcgaagta ggcaccgcaa ttacaaggtt cgaattcggc
      361 gttgcggcta tgatgcggtt gattggctcg gcgtactcgg tcttgatctt tttccgatct
      421 cggaagttcc accaatcatc gacgggcttc cacggccatt gcgagacgta aagcacgtcg
      481 tctccgaaaa ttgccatgct tttgtcgaat ccaacctgga ttccggagat cgcggtgccc
      541 atgagtgtca cagcaacgat cccgatgatg acgccgagcg cggtcagggt tgagcgcgtt
      601 ttgttcgacc gcatctgcgc agcggcgatt ttccagcttt cggtcagctc gtacagaaat
      661 cgcttcatta gtaaaccgcc gcatcggccg gcaggggatc ttctaccggc aagttcttga
      721 tgccgtcgaa cccgccgtcg tcactttcga ttagcccatc gcgcaagcgg acggtgcgcc
      781 gcgcgtgatt cgctatgtcg gcttcgtgcg taacgagaat aattgtgtgt ccgcgttgat
      841 acagattttc gaacagcgtc atgatttcat cgccggtttt cgaatcaaga ttgccggtcg
      901 gttcatcggc caggatgatg gatggatcgt ttactaaagc ccgcgcgatc gcgacgcgct
      961 ggcgttgacc gccggaaagc tcattaggtt tatggtgcaa acgatcacca agccccacct
     1021 cccgcaacac gtgcgcggcc cgctcacggc gtgtttccgg atcgatgccg gcgtaaacga
     1081 gagggagctc cacgttccgc aagctgtcag cgcgtggaag aagattgaag gtttgaaaaa
     1141 caaaaccgat ctcgcggttc cgaattgccg ccagttcatc gtcgctcatg gctgaaacgt
     1201 cccggccgtt gaattcgtag cgcccggaac tcggagtatc gaggcagccg agcatgttca
     1261 tcagcgtcga tttgccagag ccactaggcc ccatgattgc gaggtactcg ttgctgcgaa
     1321 tttgcaacga cacgccgcgc agggcatgaa ccgtttcttc tcccatcacg tagtctttcg
     1381 tgatgttctc gatgtcgatg attatcggct ccgatgatcg agtgtcactc gtcacttcgc
     1441 gacttccttg tcgtacgcga tcttcgcgcc ttcttttaac ttccggctga ttgcgctgta
     1501 gctacctgag atgacttcgt caccgggttt aatgccgctt ttgatttcca tgtaggaatc
     1561 gtcggcaatc cccgtagtta ccttcaccat ctgcgccttg ttgctgttct tgagaaagac
     1621 aaccttgacc agtctctcgc gttcttcttt ctgggtctgt ttctcaacac ggtcattgct
     1681 gagctccgcc ttgttatcgt ccttgtcgcg cgccgcgact ttctgctttt gtttttcgat
     1741 ttcctccggg ctgaggttgc tgtccccagt gcgaatcgtc acgctttgca tcggcaccgc
     1801 gacggcatcc ttaaccatgt tcgtctcgat atccgcggtg caactcagtc ccgggcgtaa
     1861 cgctacatcg tggtcgtcga cgcgaatttt cacctcgaaa ttcgtcactt cctcctgcgt
     1921 cccggttccg gtcgtctttc cggtattcgc gatttgctga acgacaccct tgaatttgcg
     1981 gtcgccgtac gcgtcgattt tgatgtttgc cttatcccct agtttcacgt ttacgacatc
     2041 attctcgttc acgtctacca cggcttgcat gtgggtgaga tcggcgactc gcatcacgtc
     2101 ggtcccggca aattgattgg tcgcgacaag gcgttcgccc agtttgctgt ttagaatggt
     2161 gacggttccg tccattggcg cataaatggt cgtttttgaa agctgatcgc gtgcctggct
     2221 ggagctggcc tcggcccgtt cgatttcgtg tagtgaactc tcgtaggtat ttttcgctac
     2281 gtcgttcgct gcttccgcgg cattgtattc ctgttctgaa atcagctttt tctcgaacaa
     2341 gtcctttgat cggcggaagt cgtgctcggt tttgagcatc gtcgcttttt gctgaaggtt
     2401 cgtcgctttg gcggagctaa tcgctgcttc ctgttgttcg agtaacgctt tgtaggaatc
     2461 aggtttaatt ttcaaaagca ggtcgccttt cttgacctgc atgccatcct ccacgggcag
     2521 ctcggtaatc tcgccggcga cttccggcga aattttcact tcggtttcgg gctggacctt
     2581 gccggtggcg gagacggtct gcagaatggt gcggcgaacc gccttttctg ttgtcaccgg
     2641 gatcggcttc tcgcgcttgc tagcgacgac cagcgcgatg atcagaagca gcactgcgcc
     2701 ggcagagcca aaaagaatct tgcgtcgccg cccttggcgg cgcttcttcg ctgtttcacg
     2761 ggcaaccgcg ggtttggtca gtgcttgatg catggcatga tcgcttttgc gggtgaagct
     2821 aactctcttt agactttcgc caatggcgca cgttcat
//
LOCUS       DASULZ010000445         4837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1375047, whole genome shotgun sequence.
ACCESSION   DASULZ010000445 DASULZ010000000
VERSION     DASULZ010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375047"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VFY96_09015"
     CDS             complement(<1..279)
                     /locus_tag="VFY96_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606641.1"
                     /translation="MKQRIELCALLILLFRFIQINSLATAATPEETLTRINRLPAAER
                     DAALIKEAKNEKTVIWYAPMNREDLRQFTAGFEAEYPFLHVEILTGGPQ"
     gene            complement(296..796)
                     /locus_tag="VFY96_09020"
     CDS             complement(296..796)
                     /locus_tag="VFY96_09020"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX5606642.1"
                     /translation="MLQEIPLAQAYRVTPTGLYLISTAYQGKRNVQFAFRALGISEAP
                     PLLLIGIQDKNFSREMIQKSGEFVLNVCSEDQLQAVDKSRDLSGREVEDKFLALGLEP
                     LPATRVQAPLVAGCHASVECRVVSDFFAEGLHLFVGEALTAHLDHQLPPVGRLAGKTY
                     RLGKAI"
     gene            complement(812..1648)
                     /locus_tag="VFY96_09025"
     CDS             complement(812..1648)
                     /locus_tag="VFY96_09025"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEX5606643.1"
                     /translation="MNDREKRARLREILAGRKGIIAPGITDALFARLAQDCGYEAIHL
                     SGNAIHKNFCLPDRNLLTVTQIAQRSAQIAEATEIPLIVDGGPACIETEALARAVKLY
                     ERAGAAALRFEDALLNEYGTPAESLAIAPLQVMLERIKAAADARQDETLVLIARCDSR
                     PKEPLSQVQERLTAYVEAGADAVGVQLIDTEEFRLIGTTAPAPLVSMWPRARMSAFEF
                     IKMGYRIALMPSSVPMAAITAARELLLELTRSGRDQDYFARQREFADSESWYKNLGTI
                     PE"
     gene            complement(1655..2494)
                     /locus_tag="VFY96_09030"
     CDS             complement(1655..2494)
                     /locus_tag="VFY96_09030"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5606644.1"
                     /translation="MMEVCNLAQSLDHFVVPVDDIVVAEEFYVRVFGGVITKRNGLNT
                     RQRKRGAVPHTFIQIGGKRMGVYLQSDERPPPVSPRGLPTYSFTTTAQGLDSVKNHLR
                     DLGSIYDGPVRRPYPFADQSLFFTDPAGNHYAVFTPAEPWRALDESAGMTGVGYIELE
                     APDLSASIEFYERVLGFQLVHRREDLPHAMLTMASGQTLILTTAPFSSKGLVMSRKVP
                     GPHIAFYVPGDRWRDALAHLDELGIAHGDRGAAKERHDGQGGTYMDDPSGYVIQFISD
                     GME"
     gene            complement(2505..3650)
                     /locus_tag="VFY96_09035"
     CDS             complement(2505..3650)
                     /locus_tag="VFY96_09035"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX5606645.1"
                     /translation="MAIQIGGDIFNREQAEALLYPAKGLPGGGQLEVRMEISADHPDP
                     DIRAVARRVKFFNLESWHKEWTEVAKKNEDLAAGFAQQNRKQTAHEFYLRAADFYRRA
                     LVYMAESDPRMLPTFKKLEENFENAWSLVKPPFERVEIPYDGHKLPALFWPGRGTPGT
                     KLPVVYNYGGADGILLRGEDGGAGQYVRRGMSFIDVDGPGHGATLRYHKLYAPPDSER
                     VAKAVIDYLVARPDVDPERIGLHGSSMGGYSGPRCVTREKRIKAVAVWSGAYNLLDDI
                     FDYYPPIQERLRWLIGAKDLKEARERIKEFTLIGRAKQIECPLLVGYSIDDRVMDPRG
                     ALNLYENAVNSPDRAMLDGVGHGQKRFDRRTYIADWFMEQLKADQPA"
     gene            complement(3790..4515)
                     /locus_tag="VFY96_09040"
     CDS             complement(3790..4515)
                     /locus_tag="VFY96_09040"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX5606646.1"
                     /translation="MTRRGSPLIGVTADLEDSPEREPRCFLLQRYLQPLTESGATAVI
                     LPPMARTAAVRRALSPLDGLVITGGNFDIHPSYYGEKPIAELGAMKSRRTEFELEIAA
                     AALRKDLPLLGLCGGEQALNVALGGSLYQDITAQVPAALEHEQSEKKAYAGHYVEIVP
                     QTRLYRVVQCDRIEVNTSHHQAVKQPGKGLIIDAVADDGVIEGIESTRHRFVLGVQWH
                     PEALAPKRQAHRRIFSAFIAACK"
     gene            complement(4512..>4837)
                     /locus_tag="VFY96_09045"
     CDS             complement(4512..>4837)
                     /locus_tag="VFY96_09045"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX5606647.1"
                     /translation="IHPTATRVAKQIGIPVPSQEMLFDPEVNLTLGTQYLKDLLARYS
                     NNRQKAIAAYNAGEAAVDRWEKEISTDDIEEFVERIPYVETRGYVKLVLRNQQIYKSL
                     YEIQK"
BASE COUNT         1010 a         1345 c         1359 g         1123 t
ORIGIN      
        1 ctgcggtccc ccggtgagaa tctcgacgtg aagaaacggg tattccgctt cgaacccggc
       61 cgtgaactgc cgcaggtcct cgcggttcat aggcgcatac cagatgacgg ttttctcgtt
      121 ttttgcttcc ttgatgagag cggcatctcg ctcagcagcc ggcaaccggt ttattcttgt
      181 cagtgtctct tccggagttg cagcggtggc gagggaattg atctgtataa agcgaaacaa
      241 cagaattaat agggcgcaga gctcaatccg ctgtttcata attcgtctcc ccgatctaaa
      301 tcgccttgcc gagccgatac gttttgccgg ccagtcgacc cactggcgga agttgatgat
      361 caagatgggc ggtgagggct tcacctacga aaagatgcaa tccttcggcg aagaaatcac
      421 tgacgacgcg gcactccacg ctcgcatggc agcctgcaac tagcggagcc tgtacccgag
      481 ttgccggcaa tggttccagg ccgagggcaa gaaacttatc ctcgacttcg cgtcccgaaa
      541 ggtcacgact tttgtcaacg gcttgcaact ggtcctccga gcaaacgttg aggacaaatt
      601 cgccgctttt ctggatcatt tcccggctga aattcttgtc ctgaatcccg atgagcagta
      661 gcggcggtgc ctcactgatg ccgagggcgc gaaatgcgaa ttgaacatta cgcttgccct
      721 gataggccgt ggaaatgaga tacaaacccg ttggggttac tcggtaggct tgtgccagtg
      781 ggatttcttg tagcattgcg tctccaaaga tctattctgg gatagtgccg agatttttgt
      841 accagctttc gctatcggcg aactcccgtt ggcgagcgaa gtaatcttga tctcgcccgg
      901 accgcgtcag ctcaagcagc agttcgcggg ccgcagtaat cgcagccatg ggaacgctcg
      961 acggcatcag agcgatgcga tagcccatct tgatgaactc gaatgcgctc atgcgcgctc
     1021 tcggccacat gctgaccaac ggtgccggcg cggtcgtgcc gatgagccga aattcttccg
     1081 tgtcgataag ttgtacgcct acggcatcgg ccccagcttc aacgtatgcc gtcaatcgct
     1141 cttgaacttg gcttagcggt tcctttggac gggaatcgca gcgcgcgatt aaaacaagcg
     1201 tttcgtcttg gcgcgcgtcg gcggcggcct tgattcgttc gagcatcact tgcagagggg
     1261 cgatcgccag ggactcggcg ggcgtgccat actcattgag gagcgcgtct tcgaagcgca
     1321 gcgccgctgc accggcgcgc tcgtacaact ttaccgctcg cgccaaggct tcggtctcga
     1381 tgcacgccgg gccgccgtcg acgatcagcg gaatctccgt ggcctccgcg atctgtgccg
     1441 aacgttgagc gatttgtgtg acggtcaaaa gattacggtc gggcaggcaa aaatttttgt
     1501 gaatcgcatt acctgacagg tgtatagctt catagccgca gtcttgggct aatcgcgcaa
     1561 acagcgcatc ggtgataccg ggcgcgataa tacctttccg gccggcgaga atttcgcgca
     1621 gccgagcacg tttttctcga tcgttcattg tgctctattc catgccgtcg gaaatgaact
     1681 gaatcacgta gccggaagga tcatccatgt aggtgccgcc ctgaccgtcg tgacgttctt
     1741 tcgcggcgcc gcggtctccg tgggcaatcc cgagttcatc aagatgcgcc agcgcatcgc
     1801 gccatcgatc tccaggaaca tagaaagcga tgtgcggtcc cggcaccttg cggctcatga
     1861 cgagcccttt tgatgaaaac ggcgccgtcg taagaattaa tgtctgtccg ctggccatcg
     1921 tcaacatcgc gtggggcaga tcttcacggc gatggactag ctggaagccg aggactctct
     1981 cgtagaactc gatcgaagca ctgagatcgg gcgcctccag ttcgatgtag ccgacgccgg
     2041 tcatccctgc gctttcatcc aaggcccgcc agggttccgc gggtgtgaag actgcgtagt
     2101 gattgcctgc gggatctgtg aaaaataaag actggtcagc gaaaggatac ggacgcctaa
     2161 ccggtccgtc ataaatgctt ccgagatcac gcaaatggtt tttaaccgag tcgagtccct
     2221 gcgctgtggt ggtgaaggag tacgtcggga gtccgcgcgg cgagacgggt ggcgggcgct
     2281 cgtcgctctg cagatacaca cccatgcgct tgccgccgat ttgaataaaa gtgtgcggca
     2341 cggcgccgcg cttgcgctgg cgcgtgttca agccgttccg tttcgtgatt accccgccaa
     2401 aaactcgcac gtagaattct tcggcaacga cgatgtcgtc cacaggaacg acaaaatgat
     2461 ccaatgactg agcgagattg catacctcca tcatggctcc gcctttaagc cggctgatca
     2521 gctttgagct gttccataaa ccagtcggcg atgtaagttc ttcggtcaaa cctcttttgg
     2581 ccatgaccga cgccgtcgag catggctcga tcgggcgagt tgacggcgtt ttcgtataag
     2641 ttaagcgcgc cgcgcgggtc catcactcga tcgtcgatgc tgtaaccgac gaggagaggg
     2701 cattcaattt gtttcgcgcg tccaatcagc gtaaattctt taattcgttc gcgagcctcc
     2761 tttaaatctt ttgcgcctat cagccagcgc agcctttctt gaatcggcgg gtagtagtcg
     2821 aaaatatcat ccagcagatt gtaggcgccg ctccaaacgg cgacggcctt aattcttttc
     2881 tccctcgtca cgcagcgtgg accggagtag ccgcccatgc tcgagccgtg cagcccgatg
     2941 cgctcgggat cgacatcggg gcgggcgacc aaataatcaa tcaccgcttt cgcaacgcgc
     3001 tcggaatctg gaggtgcata gagcttgtgg tagcgtaatg tggcgccatg ccccggacca
     3061 tcgacgtcga tgaaagacat gccgcggcgc acgtattgcc cagcgccacc gtcttcgccg
     3121 cgaagcaaaa tgccgtccgc accgccatag ttatatacga ccggtagctt ggtaccgggc
     3181 gtaccgcgtc cgggccaaaa tagtgccggc aacttgtgcc cgtcatatgg gatttccaca
     3241 cgctcgaatg gcggcttcac caaactccaa gcgttctcga aattttcttc caattttttg
     3301 aaggttggca acatgcgtgg atcgctttcc gccatataaa cgagggcgcg acgataaaaa
     3361 tccgcagcac gcaaataaaa ctcatgagca gtttgcttgc ggttttgttg tgcaaacccg
     3421 gcggcgagat cttcgttctt ctttgcgacc tccgtccatt ccttatgcca gctctcgaga
     3481 ttgaaaaact tcacccggcg tgcgacggcg cgaatatcgg gatccggatg atccgccgat
     3541 atttccattc ggacttccaa ctggccgcct ccgggtagcc ctttggcggg gtaaagcaag
     3601 gcttcggctt gctcgcgatt gaaaatatcg ccaccgattt ggatcgccat gtgatctcct
     3661 cagagcgtaa aactgctcct tctttgcctg agtaagacta agatttcaac cgggcatgat
     3721 tccgagaagg atttcggcgc gagatgagcg gaaacatttt ttcaccctgc ccaaaacggt
     3781 gtagtttttt tatttgcagg cggcgatgaa cgcggaaaag attcggcgat gggcttggcg
     3841 ttttggcgca agcgcttcgg gatgccactg cacgcccaag acaaaacggt ggcgtgtgct
     3901 ctcgattcct tcgataacgc catcatcggc aactgcgtcg atgatcaatc ctttgcccgg
     3961 ttgttttacg gcctgatgat ggctggtatt gacctctatg cggtcgcatt gaacgaccct
     4021 gtagaggcgt gtttgcggca cgatttcgac atagtggccg gcgtaggctt ttttctcgct
     4081 ttgttcgtgt tctaacgcag ccggcacctg cgcggtgata tcctgataaa gcgagccgcc
     4141 gagtgccacg ttgagggctt gctcgccacc gcatagaccg agaagcggca aatccttgcg
     4201 cagcgccgcg gcggcaattt cgagctcgaa ttccgtgcgg cgacttttca tggcgcccag
     4261 ttcggcgata ggtttttcgc cgtagtaact tggatgaatg tcgaaatttc cgccggtaat
     4321 gaccagaccg tcgagtgggc taagcgctct acgaactgcc gccgtacgcg ccatcggcgg
     4381 caaaataacc gctgtggcac cggactcggt aagtggctgc aaatagcgct gtaataggaa
     4441 acatcgcggc tcgcgctcgg ggctatcctc caagtcagcg gtaacgccaa tcagaggaga
     4501 accgcgcctt gtcatttttg aatctcgtaa aggcttttat agatttgctg attccgcagc
     4561 acgagcttca catagccgcg ggtctcgacg tacggaatcc gttccacaaa ttcctcgatg
     4621 tcatcggtgg aaatttcttt ttcccagcga tcgacggcag cttcgcccgc gttataggcg
     4681 gcaatcgctt tctggcgatt gttcgagtag cgcgccagca agtcctttaa gtactgcgtg
     4741 ccgagagtaa gattgacttc gggatcaaag agcatttcct gtgatggcac agggatcccg
     4801 atttgctttg cgacgcgggt agccgtcgga tgaatga
//
LOCUS       DASULZ010000446         3464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1171550, whole genome shotgun sequence.
ACCESSION   DASULZ010000446 DASULZ010000000
VERSION     DASULZ010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171550"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VFY96_09050"
     CDS             <1..726
                     /locus_tag="VFY96_09050"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX5606648.1"
                     /translation="TQQFGDIYAMLALGLLGWLMKQLGWPRAPFLVGFVLTKPTEQYL
                     WLSISRYDFEWLLHPGVIALGVLLIASIVWVVQGRRKGKTLGAEESGEGEVLLGKIPA
                     VLFTSAVLIVVVAALYDAQDFPYLGAIFPMAATIPAVFMAAGQLVLEMRSDIPSPPPE
                     NRKRSKLALGYLFSLVLYLLLILLIGFGVATAAFTFAFLFGWIRWQWVKALTYTVAVV
                     GIAQLMSWLLNLYWPEGMLLSLW"
     gene            720..2117
                     /gene="tcuA"
                     /locus_tag="VFY96_09055"
     CDS             720..2117
                     /gene="tcuA"
                     /locus_tag="VFY96_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEX5606649.1"
                     /translation="MVNLSAPLDVLVVGGGNAALCAAITARRAGATVLVLECATKDFR
                     GGNSRHTRDIRYMHRAATDFVTHAYGEEEFWQDLLGVTGGATNERLARLTIRESEDLA
                     DWMAANGVRWQKPLRGTLHLARSNLFMLGGGKAMMNAYYDKANKLGVEVHYGTEVREL
                     TIADGEFLSALAVSAGASRPIEAKAIVVAAGGFEANISWLKEYWGEAAENFIIRGTPN
                     NQGRMLKELLRHGVKAVGDPRGCHAVALDARAPKFDGGIVTRLDSVPFGIVVNKKPER
                     FYDEGEDFWPKRYAIWGKLIAEQPEQIAYSIIDSEALPHFMPSVFPPVEAASIPELAT
                     ALGIEPRALGAIVDEFNRSVNGGRFNPGTLDDCRTEGLEPPKSHWARPIEAPPFYGYP
                     LRAGITFTYLGVTIDEHAQVIMCDERPAKNIFAAGEMMAGNILGKGYLAGFGLTIGSV
                     FGRIAGREAARCAGF"
     gene            2104..3261
                     /gene="tcuB"
                     /locus_tag="VFY96_09060"
     CDS             2104..3261
                     /gene="tcuB"
                     /locus_tag="VFY96_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tricarballylate utilization 4Fe-4S protein TcuB"
                     /protein_id="HEX5606650.1"
                     /translation="MPDFDLVKDGARQMTICNACRYCEGYCAVFPAMEQRRVFTKADL
                     TYLANLCFDCRDCYYACQYAPPHEFGVNIPKLMSELRADTYREFTWPAILSGLFKGNA
                     LAVTVITIASIVSVLALVLLFQGANVLFATHLGAATFYRVVPFGAMTVVPSLIALYGF
                     AVFIIGAVRFWRATGGTPLQMLDFAAFWRATKDAFGLAYMKGGGAGCNYPDERFSPIR
                     MWFHHLVFYGFLLDFASTTTAAFYDHFLDWQAPYPLISLPVILGTMGGIGLMIGSAGL
                     LHLKSRSDRVPGDPSMLNMDVAFLLLLLLTSITGLLLLAFRATPAMGTLLVIHLGVVA
                     GLFITLPYGKFAHVVYRYAALLRYAIEQRRGEISQNSPNNHGEIHPDLNVG"
BASE COUNT          644 a          968 c         1018 g          834 t
ORIGIN      
        1 acgcaacaat tcggcgatat ctatgccatg ctcgcgttgg gcttgctcgg ttggctcatg
       61 aagcagctcg gctggccgcg cgcgccgttt ctcgtcggct tcgttctcac caagcccacc
      121 gaacagtacc tgtggctgtc gatcagccgc tacgatttcg aatggctgct gcatcctggc
      181 gtcattgctt taggcgtcct gttgatcgcc tccattgtct gggtcgttca agggcgccgc
      241 aagggtaaaa ccctgggagc cgaggagtcg ggcgagggag aggtgctgct cggaaaaatt
      301 cccgccgtgc tgttcacgtc ggcggtactg atcgtagttg tcgcggcgct gtatgacgca
      361 caggacttcc cttatctggg ggcgatcttt ccgatggcag cgaccattcc ggcggttttc
      421 atggctgcag gccagctcgt actcgaaatg cgatccgaca ttccatcgcc gccgcccgag
      481 aaccgcaagc gatccaagct cgccctcggc tatttgtttt ctctggtgct ctatcttttg
      541 ttgattttgc tcatcggttt cggcgtcgcc accgcggctt tcacttttgc ttttctcttc
      601 ggctggatac gatggcaatg ggttaaagct ttgacctaca ccgtggcggt cgtcggcatt
      661 gcgcaattga tgagctggct gctcaatctc tactggccgg aaggcatgct tttgtctcta
      721 tggtaaatct cagcgctccg ctcgacgttc tggtcgtcgg cggcggcaat gccgccctgt
      781 gtgcggcgat taccgcgcgg cgcgccggtg cgaccgtgct tgtgctcgaa tgcgcgacaa
      841 aagattttcg cggcggcaac agccgtcaca cgcgcgacat tcgttacatg catagagcgg
      901 cgacggattt cgtgacccat gcttacggtg aggaagagtt ttggcaggat ctcctcggag
      961 tcacgggcgg cgcgaccaat gagcgtctcg cccgtttgac tatccgcgaa tctgaagatc
     1021 tcgccgattg gatggcggcc aacggcgtcc gctggcagaa gccgctgcgc ggcacgctcc
     1081 atctcgcgcg cagcaatctg ttcatgctcg gcggcggcaa ggcgatgatg aatgcgtact
     1141 atgataaggc gaataaactg ggagtggagg ttcactatgg aactgaggtc cgggaattaa
     1201 cgattgccga tggcgaattt ctctcggcgc tggctgtcag cgccggcgca tcgcgcccga
     1261 tcgaggccaa agcgattgtc gttgctgccg gcggtttcga agcgaatatc tcttggctga
     1321 aggaatattg gggcgaggcg gcggaaaatt tcatcattcg cggcacgccg aacaaccagg
     1381 ggcgaatgct caaagaattg ctgcgccacg gtgttaaggc ggtcggcgac ccacgcggtt
     1441 gtcacgcggt agcgttggat gcgcgcgcgc caaaattcga cggcggcatc gtcacccggc
     1501 tcgacagcgt gccgttcggc attgtcgtca ataaaaaacc agagcgtttc tatgacgagg
     1561 gcgaggattt ctggcccaaa cgctatgcga tctggggcaa gctcatcgcc gagcaaccgg
     1621 agcagatcgc ctattccatc atcgattcag aagcgttgcc ccatttcatg ccgtcggtat
     1681 ttccgccggt cgaggcagcc tcgatcccgg agctcgcgac ggcattgggt atcgaaccgc
     1741 gggcgcttgg tgccatcgtc gatgaattca accgctcagt gaacggcggc agattcaatc
     1801 ccggtacgtt ggacgattgc cgtaccgaag gtttggagcc gccaaagagt cattgggcgc
     1861 ggccgatcga agcgccgcct ttctatggct accccctgcg cgccggaatt acttttacct
     1921 atctaggcgt gacgattgac gagcacgcgc aggtgatcat gtgcgatgaa cggccagcaa
     1981 agaatatctt cgccgcgggc gagatgatgg ctggcaatat tctcggcaag ggttatctgg
     2041 ccggtttcgg attaactatc ggttcggtct tcggaagaat cgccgggcgg gaggcggcgc
     2101 gctgtgccgg attttgatct ggtcaaagac ggcgcgcggc aaatgacgat ttgcaacgcg
     2161 tgcagatatt gcgagggata ctgcgcggtt tttccggcga tggaacagcg ccgcgtcttt
     2221 accaaagctg atctgaccta tctcgcgaat ctatgttttg actgccgtga ctgttattac
     2281 gcgtgccagt atgcaccacc ccatgagttc ggcgtcaata ttccgaagct catgtccgag
     2341 ctgcgcgcgg acacttatcg cgagtttact tggccggcga ttctttccgg cttgttcaaa
     2401 ggaaacgcgc tcgcagtgac cgtgattacc atcgcgtcga ttgtgagcgt tctcgcactc
     2461 gtgcttctgt tccaaggcgc taatgttcta ttcgctaccc atctcggcgc ggcgacgttt
     2521 taccgtgtcg tgccgtttgg cgccatgacc gtggtgccat cactgatcgc gctctacggc
     2581 ttcgctgtat ttatcatcgg cgcggtgcgc ttctggcgcg cgaccggcgg cactcctttg
     2641 cagatgctcg actttgcggc gttctggcgc gcgaccaaag acgccttcgg tctggcatac
     2701 atgaagggcg gcggcgcagg ctgcaattat cccgacgaac gtttttcacc gatccggatg
     2761 tggtttcatc acttggtttt ttatggattc ctgctggatt tcgcttcgac cacaaccgcg
     2821 gcattctacg atcattttct cgattggcag gcgccctatc cacttatcag tttgccggta
     2881 attctcggca ccatgggcgg tatcggcttg atgatcggca gcgctggttt gctccacctg
     2941 aaatcgcgca gcgaccgcgt tcctggcgat ccgagcatgc tcaatatgga cgtggcattt
     3001 ctcctcctgt tattgctcac cagcatcact ggacttttac tgctggcgtt ccgcgcaacg
     3061 ccggcgatgg gaacgctgct ggtgatccat ctaggcgtcg tcgctggact gttcatcacc
     3121 ttgccttatg gaaaatttgc tcacgtcgtc taccgctacg ccgcgttgct gcgctacgcc
     3181 atcgagcagc ggcgcgggga aatttcgcag aatagtccaa ataatcatgg tgagatccac
     3241 ccggacttga acgttggttg accgagatta tcggaagcta actatcaggc gagaaggcgc
     3301 tgagagacgc gcgtctcacc caaaagcgtt agctggcctg tgatctggat aaagaatgcc
     3361 agagatttct aggttcttcg ggattatcat ttgcatgttc gtcgaggccg gaggacagca
     3421 tcatattcct cacttcgtac tatcagcagc atgtcggaat ttac
//
LOCUS       DASULZ010000447         3432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1068791, whole genome shotgun sequence.
ACCESSION   DASULZ010000447 DASULZ010000000
VERSION     DASULZ010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068791"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VFY96_09065"
     CDS             complement(<1..497)
                     /locus_tag="VFY96_09065"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEX5606651.1"
                     /translation="MSLAQSLMARARHRLGSVLLALLWLGAAANLRADEKSLLWKISK
                     GPSSVYVLGSIHYLRSENYPLKKAILEAFETSKRLVLEIDLNATSGETAQRLMLEKAL
                     FRDGTSLPQNISAETYQLAAERATQLGIDMRIFNPMKPWFAAINLIAIKLKALRLDPR
                     RGVDQ"
     gene            complement(570..893)
                     /locus_tag="VFY96_09070"
     CDS             complement(570..893)
                     /locus_tag="VFY96_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5606652.1"
                     /translation="MGVSYIPVARAGELSPGAMKEIDLGGRQALLAYVDGKYFAFSRH
                     CPHEEADLKTAGQLLNGTTIRCNNHSYCYDLNSGECTLPKGAAPLTVLPAEERGEEIC
                     IRLEW"
     gene            complement(905..2602)
                     /locus_tag="VFY96_09075"
     CDS             complement(905..2602)
                     /locus_tag="VFY96_09075"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5606653.1"
                     /translation="MPYDLMIRGGRVVDGSGLPSYVADVGVKNGRIAEVGRLTESAAR
                     TIDAAGLAVSPGFIDHHTHLDAQMLWDPYGTCEPQHGITTVVMGNCGLTLAPIKKGDE
                     DAVVKSFVRVEAIPRHALEQGVTWNWHSYGEYLDNFEGKVGINVGGLVGHIAVRHNVM
                     GEEAVERQATSAEVQKMRALILESMQGGALGMSTNRNERHMREDGKPVASRLANDEEL
                     FALCDVLAERNAGVIETILGRNKIEHFEFYHELAKRTQRPVLWQSLQHRWVEPNLWRE
                     QLNAVEPIFKAGYQAYGLSHTVPLVRHFTLQDCQVFDEFPTWKNLMFLPVEVRKQALA
                     DPDTRRKLRADFADPRPTNFHRRWDIPRVEKVVKPENQKYVGKTLADLAALRNQDPLD
                     AFLDLALEENLGTVFWNANNGGDWDAMGEILRSPYVLVGTSDAGAHVQFGADFGYGTI
                     LLGLWVRERQVMSLEQAIHKLTFHVASIFALEGRGLLRPGYAADLTIFDPKTVGAHEP
                     EWANDYPAASKRLIQKSAGLHYTIVNGRVIHEDGRMTGDLPGQVIRGPLYREQKAAA"
     gene            2820..>3432
                     /locus_tag="VFY96_09080"
     CDS             2820..>3432
                     /locus_tag="VFY96_09080"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX5606654.1"
                     /translation="MADVTDMRGYIELLESMGELRRIDGADLNTEVGALTELAADKQG
                     PALLFDNFKQYPAGFRVISNVFRTCKRTGPAMGIDAENGIDYLYAWRKKLAAYKPVPV
                     QQVEGGPVMENQMTDEEVDLYKFPTPRWHDLDGGPFIGTGCGIITKDLETGKINIGTY
                     RVMVQDKNHVSVKMNMGKHGRLAFERARDAGKALPIAITLGQGP"
BASE COUNT          740 a         1017 c          920 g          755 t
ORIGIN      
        1 tattggtcga caccgcgcct gggatcgagt cggagcgctt tcagttttat ggcgatgaga
       61 ttgatcgccg cgaaccaggg cttcatcggg ttgaaaatcc gcatgtcgat cccgagttgc
      121 gtggcgcgct ccgccgccaa ctgataggtt tccgcgctga tattctgcgg caaactcgtt
      181 ccgtcccgaa agagcgcttt ttccagcatc agtcgttggg cagtttcccc cgacgtggcg
      241 ttgagatcga tctccagcac cagcctcttg ctcgtttcaa acgcctcgag aatggctttt
      301 ttgagcggat agttttcgct gcgcaggtag tgaatcgacc ctagcacata gacgctgctc
      361 ggccccttgc tgatcttcca gagaaggctc ttttcatcgg cgcgaagatt tgcggcagcg
      421 ccgagccaaa gcaatgcaag tagaacgctg ccgagccgat ggcgcgcacg cgccatgaga
      481 gattgagcta gactcatctt agagcaacgg aaggccgtaa cgacaagctt gtcgttaccg
      541 gtgagtgaca atggccagga gatcagaacc taccactcca ggcgaataca aatctcctcg
      601 cctcgctcct cggcaggtaa aaccgtcagc ggcgctgcgc ccttgggcaa agtgcattcg
      661 cccgagttga gatcatagca ataactgtga ttattacagc gaatggtcgt cccgttgagc
      721 agctgtccgg cggttttcaa atcggcctct tcgtgcgggc agtgtctcga gaacgcgaag
      781 tattttccgt ctacgtatgc caacagggcc tgccgcccgc cgaggtcgat ctctttcatc
      841 gcgcccggac ttagctcgcc cgcgcgcgca actgggatat agctgacccc catgatcgtc
      901 gatgctaggc agcggccttt tgttcgcgat agagcgggcc ccgaataacc tgccctggca
      961 ggtcgccggt catgcgaccg tcttcgtgaa tcactcggcc gttaacgatt gtatagtgca
     1021 gtcccgcaga tttctgaatc aatcgcttgc ttgcggccgg ataatcattc gcccattccg
     1081 gttcatgcgc gccgacggtt tttggatcga agatggtcag gtcggcagca tagcccggcc
     1141 gcagcaagcc gcgcccctcg agagcgaaaa tcgaagcgac atggaacgtc agtttgtgga
     1201 tcgcctgctc gaggctcatg acctggcgct cgcgcaccca tagtcccaaa agaatcgtgc
     1261 cgtagccgaa gtcggcgccg aattgcacat gggcaccggc gtccgaagtg ccgaccagta
     1321 cgtaaggact gcgcagaatt tcgcccatgg cgtcccaatc gccgccgttg ttcgcgttcc
     1381 aaaaaaccgt gccgagattt tcttccaaag ctaggtccaa aaaggcatcg agcggatctt
     1441 gattgcgtag cgccgccaga tcggcgagag tcttgccgac atatttctga ttttccggct
     1501 tcaccacctt ttccacccgc ggaatgtccc agcgccggtg gaagtttgtc ggccgtggat
     1561 cggcaaagtc cgcgcgcaac tttcgcctcg tgtccgggtc tgccaacgct tgcttacgaa
     1621 cttccaccgg caggaacatc aaattcttcc aggtcggaaa ttcatcgaaa acctggcaat
     1681 cttgcaatgt gaaatggcgc accagcggta ccgtgtggga cagaccatag gcttgataac
     1741 ccgctttgaa aatcggctcc accgcgttga gctgttcgcg ccagagattg ggctcaaccc
     1801 agcggtgctg caggctctgc cataggactg ggcgctgcgt acgtttggca agctcatgat
     1861 aaaattcgaa atgctcgatt ttattgcgcc cgagaatcgt ttcgatgacg ccggcattgc
     1921 gctcggcaag aacatcgcac agggcaaaaa gctcttcgtc attcgccaat cgactcgcca
     1981 ccggcttccc gtcttcgcgc atatgccgct cattgcgatt ggtcgacatt cccaacgcgc
     2041 cgccttgcat cgattctaga ataagcgcgc gcatcttttg cacttccgcg ctggtcgctt
     2101 gacgctcgac ggcctcttct cccatcacgt tgtggcgcac ggcaatgtgg ccgaccaggc
     2161 cgccgacatt gatgcccacc ttgccttcaa agttatcgag atactcacca tagctgtgcc
     2221 agttccatgt aacgccctgt tccagagcat gccgcggaat cgcttcgacc cgaacgaagc
     2281 ttttcaccac cgcatcttca tcgccttttt tgatgggtgc caaggtcagg ccgcaatttc
     2341 ccatcaccac tgttgtaatc ccatgctgcg gctcacaggt gccgtatgga tcccagagca
     2401 tctgcgcgtc caaatgagtg tggtgatcga taaatcctgg agaaaccgcc aaaccggcgg
     2461 catcgatcgt gcgcgcggcg ctctctgtta agcgtccgac ttcagcgatc ctgccgtttt
     2521 tgacgccgac gtcagccaca taagatggaa gtccggagcc atcgacaaca cggccaccgc
     2581 gaatcatcag atcgtaaggc atttatttta tcctccgtcg ctaataaaaa ctcgaccttt
     2641 ggcgacaact atcatgtctg caccatatgc aatgacaaga aggtcgtcaa ccattcgttg
     2701 cgtcgcggcg cgcactaaga agctcgcctg cggctttcca cgatgttttt acattcgcga
     2761 cgtcgtgttg actcgccctc accatcaact tagtatgacc gtcgatttga ggaggtcgca
     2821 tggctgatgt caccgacatg agaggctaca tcgagttatt ggaatccatg ggcgagttgc
     2881 gccgcattga tggcgctgac ttgaacaccg aggtcggagc cttgacggaa cttgccgccg
     2941 ataagcaggg acctgcgctt ctgttcgata atttcaagca gtaccccgcg ggatttcgag
     3001 tcatttccaa tgtctttcgc acctgcaagc gcaccgggcc ggcaatggga atcgacgcag
     3061 aaaacggcat cgactatctt tacgcctgga gaaaaaagct ggccgcctac aagccggtac
     3121 ccgtgcagca ggtcgaaggt ggtccggtga tggaaaacca aatgaccgac gaggaggtcg
     3181 atctttacaa gtttccgacg ccgcgatggc atgatctcga cggtggcccg tttatcggca
     3241 ccggctgcgg catcatcacc aaagaccttg aaaccggcaa gatcaacatc ggcacttacc
     3301 gcgtcatggt gcaggacaaa aatcacgtct cggtcaaaat gaacatgggg aaacacggtc
     3361 gcctcgcttt cgaacgcgca cgcgacgccg gcaaagcgct gccgatcgca atcacacttg
     3421 gccaaggccc gg
//
LOCUS       DASULZ010000448         7811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_765124, whole genome shotgun sequence.
ACCESSION   DASULZ010000448 DASULZ010000000
VERSION     DASULZ010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7811
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765124"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VFY96_09085"
     CDS             <1..551
                     /locus_tag="VFY96_09085"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5606655.1"
                     /translation="SPHAVTVGREHPNHNYVEIAKGNPVILRGLGNYGAEWNKISTTV
                     RDVLIYSVPVYQQQFGNALKGAAFVFDLGTLCIAHLGDLSHKLTEEQIKAFGKVDIAM
                     IPIGGTFTMPPDTAREVLQQLKPKIAIPMHYRESTYLLDMFVKNYSHKYLSTFRMTFS
                     KAALPPPTQIVIFTPWGMRDYR"
     gene            complement(613..1602)
                     /locus_tag="VFY96_09090"
     CDS             complement(613..1602)
                     /locus_tag="VFY96_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX5606656.1"
                     /translation="MTHLRLTLACEDYDRTRPIRDGIVKPDGIELNYLTMSVEEIFWR
                     MMKYEEFDVSELSMGAFLTAAARGHRPFVAIPVFPSRTFRHRCIFVNTAAGVRRVEDL
                     RGRRMGVPEYSMTAAVWLRGLFQHEYGVKPGDIHWVQAGEEHPGRKDRVDFEMPPGVR
                     LESRPNTTLNAMIEQGEIDAMMSPRMPSCFLQGSPRVERLFPDYRQVEMDYFRRTGLF
                     PIMHVIVIRRSIYEMERWVAQTLYKAFCDAKDICMGDLYDTNILRVSLPWTSAEYEDT
                     RDLMSVDYWPYGLEPNRTNLETLHRYLFEQGLIEQRLNLDALFARETADAFKI"
     gene            complement(1599..2267)
                     /locus_tag="VFY96_09095"
     CDS             complement(1599..2267)
                     /locus_tag="VFY96_09095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEX5606657.1"
                     /translation="MNRTELTSNLYRHRLVAVVRSKTVDEALDLADAAAEAGIQFIEI
                     TFSVPGALDVIKELAARRELRVGAGTVLAPQQAERAIAAGAQFVVSPSLEINLIRICH
                     AANVACFPGAATPTEIIAAHRARADLIKIFPADLVGGAYFIRQMHGPFPEIRFMVSGG
                     VSLKNVSEYVEIGVAGICLGSSYLGELLAKAGKKAFVREVRKFVSIVDKTQLKETNLR
                     KRQS"
     gene            complement(2451..3065)
                     /locus_tag="VFY96_09100"
     CDS             complement(2451..3065)
                     /locus_tag="VFY96_09100"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606658.1"
                     /translation="MRGATFGTSSLSGGIITALKEALKLKGLEYPRDYKLLVIAGGSS
                     ANLAALQSGQIAATTVAVPLNYAAEESGLNIIGRLSEGIPHFQTNALVTRRAWAEKNR
                     QLAIRFMKAMVLTFRWMFQNPDAAIDFLSKEMQLKPMHARKGWEFYTQNRFWPPDGDV
                     TMEGLKNNIRIYAEQSGAKGPLTNAAKYVDQSYLQDALKELEKN"
     gene            complement(3032..3430)
                     /locus_tag="VFY96_09105"
     CDS             complement(3032..3430)
                     /locus_tag="VFY96_09105"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606659.1"
                     /translation="MLGQSRTMVAFLVILIWLTRGHAQEKPKIFVGASSKTLGYSPLW
                     IATKKGFFDQQGLDVQLVLLRGVPMTLQALAAGSLHFGSGGPEAYIEASERGLDFVVT
                     GGIINGMAQFLIAGKNYKKICAVRPLVHLP"
     gene            3704..3961
                     /locus_tag="VFY96_09110"
     CDS             3704..3961
                     /locus_tag="VFY96_09110"
                     /inference="COORDINATES: protein motif:HMM:NF024833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HEX5606660.1"
                     /translation="MEIIKSRFSRWALIITITAATAGCAGGPLTSREKGAGIGALGGA
                     AAGAAIGSAFHRPGMGAAIGAGTGLGAGALIGDYMQGQQER"
     gene            4058..4582
                     /locus_tag="VFY96_09115"
     CDS             4058..4582
                     /locus_tag="VFY96_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606661.1"
                     /translation="MNRSKVLVLGGLLTAALIVPAIPALADKEHFEHHENNGKHKGWE
                     KSAGKKFDEHDRRNYRHPDRRFSHRDTRYDYRGHHNKAEIRQDFKDVRSARKEVSDSR
                     AELHKDYTELRKDRMELRRDLRNGASKAEILKDRQEIRGDLKEIHGDRVDLRNDQAKL
                     DAARRELKSDIRKR"
     gene            4783..4905
                     /locus_tag="VFY96_09120"
     CDS             4783..4905
                     /locus_tag="VFY96_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606662.1"
                     /translation="MAAQLYNAGIQRAEGLLEIAAGLYDSSLFYETRRAAETDF"
     gene            5005..6552
                     /locus_tag="VFY96_09125"
     CDS             5005..6552
                     /locus_tag="VFY96_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEX5606663.1"
                     /translation="MDPQASVTPYYNAAVDLIERNLVARPKKIAYIDADGSYTFAELA
                     ERVNRCANALTQLGLEMESRIVVALLDTIDFPAVFLGAIKAGIVPIAVNTLLTSADFD
                     FILRDSRARALIVSAPLLPAFLPILNNQPFLKHVVVSTEQRSDYPRLSDMTAKLATTF
                     VAAPTRQDDACFWLYSSGSTGTPKGVVHAQTSMIQTAELYAKPVLGIQENDIVFSAAK
                     LFFAYGLGNGLTFPLSVGATTVLLADRPTPQAVCRILRQYKPTIFYGVPTLYSALLSC
                     PDLPKKSELSLRRCTSAGEALPPEVGRRWLERTGVDILDGLGSTEMLHIFLSNRPAEV
                     RYGTSGKPVPGYEVRLLDEAAKPVNRGEIGELQVRGPTSASYYWNKREKSRQTFLGPW
                     TRSGDKYSETEDGYFVYSGRNDDMLKVGGIWVSPFEVESALASHAAVLETAVVAHPDE
                     NGLIKPKAYVVLCPNITATEKLAAELQRHVKDQLAPYKYPRWIEFVAELPKTATGKIQ
                     RFKLRTN"
     gene            complement(6667..7410)
                     /locus_tag="VFY96_09130"
     CDS             complement(6667..7410)
                     /locus_tag="VFY96_09130"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEX5606664.1"
                     /translation="MRLLGKAAIVTGGARHIGAAYCRRLAKEGAAVVIADILDGQSVA
                     NEIRSNGGKAIALKIDVSNENDTERMAAQTIQTFGAIDILVNNAAIFINLDRHPFYEI
                     TAAEWDRISAVNIKGPFLCAKAVFPQMKSQRSGKIINISSSTAYWGTPDFLHYVASKA
                     ALIGMTRSLAREVGEYRICVNAIAPGLVEHEGQNAPKALTELQLKARSIKRLQTPEDL
                     LGTLVFLASSDSDFMTGQTIVVDGGSVFH"
     gene            complement(7514..>7811)
                     /locus_tag="VFY96_09135"
     CDS             complement(7514..>7811)
                     /locus_tag="VFY96_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007236381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606665.1"
                     /translation="IDSTTPLDIERHDKNMYCGDWMIGEYSGDQALENRPFPGWGQYR
                     TPIDGLFMCGSSCHPGGNITGAPGYNAAKVIAESIGIKPWWKPVEFREHLRGLT"
BASE COUNT         1830 a         2248 c         2013 g         1720 t
ORIGIN      
        1 tttcgcccca tgccgtcacc gtcggtcgtg agcatcccaa tcacaactat gtcgaaatcg
       61 ccaaaggaaa tccggtgatc ctgcgaggac tggggaatta cggggctgag tggaacaaaa
      121 tcagcacgac cgtccgcgac gtcttaatct acagtgtccc ggtttaccag cagcaattcg
      181 gcaatgctct aaagggcgcg gcgtttgttt ttgaccttgg gacgctgtgc attgcccatc
      241 taggtgattt gagccacaag ctgaccgaag aacaaatcaa agccttcggc aaggtggaca
      301 tcgccatgat cccgatcggc ggcactttta caatgccgcc ggacaccgcg cgtgaagtgc
      361 tgcaacaact taaaccgaaa atcgcaatcc cgatgcacta cagagaaagc acctacctgc
      421 tcgacatgtt cgttaagaat tattcccaca aatatctcag caccttccgg atgacattca
      481 gcaaagcggc gttgcctcca ccgacacaaa tcgtcatttt tacgccgtgg ggaatgcgcg
      541 actacagatg aacgcgttgt tgtccaccgt ccaattttct cgcgtcgctc gcgccttgct
      601 gcggttgtat ccttaaatct tgaaagcgtc ggccgtttct cgcgcgaaga gcgcgtccag
      661 attgagtcgt tgctcgatca gtccctgttc aaacaaatac ctgtgcagcg tttctagatt
      721 agtgcggtta ggctccaaac catacggcca ataatcgacg ctcatgagat ctcgtgtatc
      781 ctcgtattcg gcagaggtcc agggcagact cacacgtaaa atattggtgt cgtacagatc
      841 acccatgcaa atatccttgg cgtcgcagaa cgccttgtag agcgtttgcg cgacccagcg
      901 ctccatttca tagatcgatc gacggatcac gatcacgtgc atgatcggaa ataagccggt
      961 tctcctgaaa tagtccattt cgacctgtcg ataatccggg aacaagcgct ccactcgtgg
     1021 cgagccttgg agaaaacagc tgggcatgcg cggcgacatc atcgcatcga tctctccctg
     1081 ctcgatcatc gcattgaggg tcgtgttcgg ccggctttcg agccgcactc ccggaggcat
     1141 ctcgaaatcc acacggtctt tccgacctgg atgttcctcg ccggcctgaa cccagtgaat
     1201 gtcgcctggc ttgacgccgt attcatgttg aaacaatcca cgcagccaca ctgccgcggt
     1261 cattgaatat tcgggcacgc ccatacgtct gccgcggaga tcctccactc gccgcacgcc
     1321 agcagccgtg ttgacaaaaa tgcagcgatg acgaaaagtt ctcgacggaa acaccggaat
     1381 tgccacgaag ggtcgatggc cgcgggcggc tgcggttaaa aacgctccca ttgacagctc
     1441 ggagacatcg aattcttcgt atttcatcat gcgccaaaaa atctcttcca ctgacatggt
     1501 caggtaattg agttcaattc cgtcgggttt gacgataccg tctctgatcg gccgcgtgcg
     1561 atcgtagtct tcgcaggcca gcgtgaggcg aaggtgtgtc atgactgccg ctttctaaga
     1621 ttcgtctcct tcagttgcgt cttgtccaca atgctgacga acttccgaac ttctctcacg
     1681 aacgctttct tgccggcttt tgcgagcagt tcgccgagat aactgctgcc caaacaaata
     1741 ccggcaacgc caatttccac gtattccgaa acgttcttca agcttactcc gcccgaaacc
     1801 atgaaacgaa tctccggaaa tggcccgtgc atttggcgaa taaagtatgc gccgccgacc
     1861 aggtcggcgg gaaagatttt tattaaatcg gcacgcgcgc ggtgcgccgc gatgatctcc
     1921 gtgggggtcg ccgcccccgg aaaacaagcc acatttgcgg catgacaaat gcggatcaag
     1981 tttatttcta gggatggcga taccacaaat tgcgcgcccg cggcgatcgc tcgctcagcc
     2041 tgctgcgggg ccaaaaccgt tccagcaccc acacgcagct ccctacgagc cgcgagttct
     2101 ttaatcacgt cgagtgcccc tggcacgcta aaagtgattt cgataaattg aatccccgct
     2161 tccgccgcgg cgtccgccag gtccagggcc tcgtccacag tcttggagcg aaccacggca
     2221 actagccgat gtcgatacaa attgctggtg agctcagttc gattcattac tttttgggtg
     2281 cggctttgct ccgcaaaata gatttctctc cgataatctc attgatgggt tgaaatgggg
     2341 atcgaatatg atggggtgca aaattaaagc gactactgag aagtaccatc ggagaaacac
     2401 aagttcaaga aactgagttc aaccctccat aatagcgctg aatccgaccg tcagtttttc
     2461 tcaagctcct tcagcgcatc ttgcaaatag ctctgatcca catacttagc ggcgttcgtc
     2521 agcggcccct tcgcaccgct ctgctccgca tagatgcgaa tgttattttt gagcccctcc
     2581 atggtcacgt caccgtccgg tggccaaaac cggttctgcg tataaaactc ccaaccctta
     2641 cgcgcgtgca tgggcttgag ctgcatctct ttggacagaa aatcgatcgc tgcatcgggg
     2701 ttctgaaaca tccagcgaaa ggtcaagacc atcgctttca tgaagcgaat tgccagctgg
     2761 cgattcttct ctgcccacgc gcgccgggtc accagcgcgt tcgtttgaaa atgcggaatg
     2821 ccttcgctca gccgaccgat aatattgaga cctgactctt cagcagcata attgagcggc
     2881 accgcgaccg ttgtcgccgc gatctggcct gattgtaagg cggccaaatt cgccgaggag
     2941 ccgcccgcga tcacgagaag tttgtaatcc cgcgggtatt caagtccctt gagcttgagc
     3001 gcttctttca gcgccgtgat gattccgccg ctcagggaag atgtaccaaa ggtcgcaccg
     3061 cgcatatctt cttgtaattt tttccggcga tgagaaattg cgccatgcca ttaatgatcc
     3121 cgccggtgac gacaaagtcg agcccccgtt cggaggcttc gatgtatgcc tcgggcccgc
     3181 cggaaccaaa gtggagtgac cctgccgcca atgcttgcaa tgtcatgggc acgccgcgca
     3241 aaagcaccag ttggacgtcg agtccctgct gatcaaaaaa tcccttcttc gtggcaatcc
     3301 aaagcgggct ataccccaaa gttttactcg acgctccgac aaagattttg ggcttctcct
     3361 gagcgtgccc gcgggtaagc catatgagaa tgaccaaaaa tgcaaccatc gtcctcgatt
     3421 gaccgagcat gcgccttccc tttccgccaa tctctctgcc taagctctac aacagcgcag
     3481 gcgcgcgata caacaagatc ttcccgacga gcggtgctgc atcagccggg gcgcacagca
     3541 cattttcact ccccggcggt aatttttact tggaagatct gtgcagatcg aagagcactt
     3601 aggcgatttc gatcagctga cgtcccacgg ttccctggca taaccgttgc tcaacccgct
     3661 ggccgaccga ccagaaaaca atcacgacta ggaggcaaat gaaatggaaa ttataaaaag
     3721 cagattttct cgctgggcgt tgatcataac gatcactgcg gcgaccgcag gctgcgccgg
     3781 cggtcccctg acaagtcgcg aaaaaggcgc gggcatcggt gctctaggcg gagctgcggc
     3841 tggcgccgcg atcggcagcg catttcaccg tcccggcatg ggcgcggcaa tcggcgctgg
     3901 taccggactc ggagcgggcg cgctgattgg ggattatatg caggggcagc aagagagata
     3961 gacactcaag ccagttttga cgagagaaat cgtcgattcg aggagctaag caggctcaat
     4021 ctaacaaaaa cgaaaaaaac gaaaggagaa tttagatatg aatcgctcaa aagttttagt
     4081 cttaggaggg ttgctgaccg ccgctctaat cgtcccggca ataccggcct tggccgataa
     4141 ggaacacttc gagcatcacg aaaacaacgg caagcacaag ggctgggaaa agagcgctgg
     4201 aaaaaaattc gacgagcacg accggcgcaa ttaccggcat cccgatcgtc gcttctctca
     4261 tcgagatact cgctacgatt atcgcggcca tcataacaaa gcggagatac gccaggactt
     4321 caaagacgtg cgcagcgcgc gcaaagaagt aagcgacagc cgggccgagc tgcacaagga
     4381 ctataccgag ctcagaaaag accgcatgga gcttcggcgt gatctgcgca acggcgcgag
     4441 caaagctgag atcctaaaag atcgccagga aatcaggggt gaccttaaag agatccacgg
     4501 tgaccgcgtg gacctccgca atgaccaggc aaagctcgac gctgcacggc gtgagttgaa
     4561 aagcgacatc cgcaaacgct aaaccactct ccttcattcc ttcatccaat cacccgatcg
     4621 tcaatgtgaa ttgacgtcgg gtgatcttgt tctattaccg tcgcgcgatc tgctgccttg
     4681 atctttgtcc gtccgctgac gaaaaactcc tcgcacaaac tgcttgtcac agccgggccc
     4741 gggtgattat tgtgagacga ctcacagggg gactccactc acatggctgc gcaactgtat
     4801 aatgccggca ttcagcgcgc cgaaggtctg ctcgagatcg ccgcgggact ttacgacagt
     4861 tcgttattct acgaaacccg acgtgctgcc gagacggatt tctgagcgag gttgccgaac
     4921 atttcaggta tatagtaaaa aaataccgct ggactgcatt gccgaacggg cacaccgtcc
     4981 gcatcgcaat taaaggaggc tgcaatggat ccccaggcat cggtcacacc ttattacaat
     5041 gcggccgttg atctgatcga gcgaaacctc gtagcgcgcc cgaaaaaaat cgcttacatc
     5101 gacgcagacg gtagctacac gtttgcagag ctggccgagc gtgtaaaccg ttgcgccaat
     5161 gcgttgaccc aactcggtct ggaaatggaa tcgcgcatcg ttgtagctct tctcgatacc
     5221 atcgactttc ccgccgtgtt cctcggtgcg ataaaagccg gcatcgtgcc gattgctgtg
     5281 aacactctac tgaccagtgc cgattttgac tttatcctgc gcgacagtcg cgcccgggct
     5341 ttgattgtct ctgcgccgtt actgccggca ttcctgccga tcttgaacaa tcagccgttt
     5401 ttgaagcacg tggtggtttc aaccgagcag cgctcggatt atccacgtct ctccgacatg
     5461 acagcgaaac tagcgacgac ctttgttgct gcgccgacgc ggcaggatga cgcttgcttc
     5521 tggctatact cgtcgggctc caccgggaca ccgaaaggcg tggtacacgc gcagaccagc
     5581 atgatacaaa ccgccgaact atacgccaag ccagtgctgg gaattcagga gaatgacatc
     5641 gtgttttcgg cggcaaagtt atttttcgca tatggtctcg ggaacggcct cacattcccg
     5701 ctttccgttg gcgccacaac agtgctgctt gccgatcggc caacaccgca ggcggtttgc
     5761 cggatcttgc gccaatacaa accgactatt ttttacggcg ttcctactct gtacagcgcg
     5821 ctactttctt gtcccgatct gcccaaaaaa agtgagctca gtctccggcg ctgcacttcg
     5881 gcaggcgaag cattgccgcc agaagttggt cggcgttggc tcgaacgcac cggcgtggat
     5941 attcttgacg gcctcggctc gacggaaatg ctacacattt ttttgtcgaa ccggcctgcc
     6001 gaggttcgtt acggaacttc tggcaaaccg gtgcccggct atgaggtgcg tctgctcgat
     6061 gaagccgcca agcccgtgaa ccgcggtgaa atcggcgagc tgcaggtccg cgggccaacg
     6121 agcgcgagct actactggaa caaacgcgag aaaagccgcc aaacgtttct cggtccatgg
     6181 actcgaagcg gtgataagta cagcgaaaca gaagacggtt attttgttta ctcgggccgg
     6241 aatgatgaca tgctcaaggt gggcggcatt tgggtttcgc cgttcgaagt cgaatcggcc
     6301 ttagcctccc atgccgcggt gctcgaaact gccgtggtgg cgcatccgga cgaaaacggt
     6361 ctgattaaac ccaaagcgta tgttgttctt tgtccgaata tcacggcaac ggaaaaactg
     6421 gcagccgagt tgcagcggca tgttaaagat caactcgcgc catacaagta cccgcgctgg
     6481 atcgaatttg tcgccgagct gccgaaaacc gcaactggta agattcagcg ctttaagctt
     6541 cgcaccaact aggaaaagaa tcggactgga gatcgactgc cccgacactg gccgtcgata
     6601 gggacacgcc gcgttatctc aggcgatcgt tatctttttc gctgaaacct ctatgaatct
     6661 tccagatcag tgaaaaacgc tgccgccatc caccacgatc gtctgccccg tcatgaaatc
     6721 gctgtcggac gatgcgagaa ataccaacgt gccaagtaaa tcctccggag tctgcaagcg
     6781 cttgatcgag cgcgctttca actgcaattc cgtgagggcc ttgggcgcat tctgcccttc
     6841 gtgctcgacc aagcctggcg caatcgcatt gacacaaatg cggtactctc ccacctctcg
     6901 cgccaacgag cgggtcatgc cgatcagcgc tgctttggat gcgacgtaat gaaggaaatc
     6961 cggcgtgccc caataagccg tcgacgatga aatgttgatg atttttccgc tacgctgaga
     7021 cttcatctgc gggaaaactg ctttggcaca aagaaacggt ccctttatat tcactgccga
     7081 tatgcggtcc cactccgccg cggtgatttc ataaaacgga tgccggtcga ggttgatgaa
     7141 gatcgccgcg ttattgacga gaatatcgat cgctccaaaa gtttgaatgg tttgggctgc
     7201 cattcgctcg gtatcatttt cattcgacac atcgatcttg agcgcaatgg ctttgccgcc
     7261 atttgagcga atttcattcg ccacagattg gccgtcgaga atatcggcaa tcaccaccgc
     7321 ggcgccttcc ttagcgagtc tccggcagta cgccgcgccg atatggcgcg caccgccagt
     7381 cacaatggcc gctttgccca acaacctcat accgagtccc cggagtgatg gaacgttgga
     7441 gcacgggaga attgaggttc agatcccgac acatcactcc accactccat tactccatca
     7501 ctccaattca tcttcatgtc agtcccctca gatgctcgcg aaactcgact ggtttccacc
     7561 atggcttaat acctatagat tccgcgatca ccttcgccgc gttgtagccc ggcgcgccgg
     7621 tgatgttgcc gccgggatgg catgacgaac cgcacatgaa tagtccatcg atcggtgtgc
     7681 gatactgccc ccatcccgga aatggccggt tctccaaagc ttgatcaccg gaatattcac
     7741 cgatcatcca atcgccgcag tacatatttt tatcgtgccg ttcgatgtcg aggggcgtcg
     7801 tcgaatcgat c
//
LOCUS       DASULZ010000449         3094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_664274, whole genome shotgun sequence.
ACCESSION   DASULZ010000449 DASULZ010000000
VERSION     DASULZ010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664274"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1768)
                     /locus_tag="VFY96_09140"
     CDS             complement(<1..1768)
                     /locus_tag="VFY96_09140"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5606666.1"
                     /translation="MEALADTIRSILIFSGLSREDVAKVLGKMEEISFESGAIIISQG
                     DQGDAFYLIQSGSVQVVLENGPDKSEVVAILGARDWFGEMALLSGEPRSATIRAVKQT
                     SLLRLRREDWDELIDKHPTWLLQLCATLSKRLSYVDRQYSTGREAFNSLAEEFYSARS
                     PQEQAFFRQASLLGAADEATWAELFPKDQPAHLVSELGNSQFPLIRRLEDGKYELHSF
                     FRDFLREKLSAAEGEEMTQRLHAVFAERYEALGNLREAVHHRVEAQDWSNAIRLLTAS
                     EHLLSEAPLYVKTILDKFPPEQLLPDLRLVNLRASALIQLGDLAGAYRSYREVLTQKA
                     SGALAADGIAGYRRMAEMLAKRNDYGQAINQLRSALNLISQESAASNDYQERAQEISL
                     PAFPTISPPAGTKPQSRSIVKRWNAIFGSPSFSKWFGAVLGIGVGSYLWFGQPEIGLD
                     AAATKLLGLICFTLIFWVFRVLPDYGVALVFGMVIILTKLESPQTVMGGFASTTWFMT
                     LGVLGLGAAITGSGLFYRFSLHLVRMFPLSYYWQIIATGVMGVVVMALIPQQTARTVI
                     TSQMLVNLSESLGYKTPSKASTG"
     gene            complement(1781..2776)
                     /locus_tag="VFY96_09145"
     CDS             complement(1781..2776)
                     /locus_tag="VFY96_09145"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606667.1"
                     /translation="MARMSGGKIWFALFVTGLSLLLAAPVLVLAQSPIRVGILVQEME
                     RSQTQAIKGLGEELKRLGYEEKKNLFFETRNVKGNRAVLQPAAGEILARKVNAIFTTG
                     TSATRAVIAATKEIPIVFVHPDDPVAAGFIKSNSERPPNLTGVAAYARATTEKRVELI
                     KEIVPGLRKILVFYDGNNAFSRDSFAAAEAAAKKFSLEAVGYAIKSTDELKTTVASLH
                     AMPATAIFQIPDELFESESEFLFETARAKKFPTMFNQETWAIGGALAAYGPNFIAMGR
                     QAGGLIHKIVKGEKPETLAIERAAKFDLTLNYRTARFLDLKFTPEMLKKADKVIR"
     gene            complement(2804..>3094)
                     /locus_tag="VFY96_09150"
     CDS             complement(2804..>3094)
                     /locus_tag="VFY96_09150"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5606668.1"
                     /translation="GYPEVIEFARGADLLIHDGQYLSDEYFSRTKPRKGWGHSTIELA
                     IEVAKKAGVKQLALFHHEPTHDDKTMATIEQRAKQIFQSTVVAHEGMEIVLR"
BASE COUNT          681 a          862 c          868 g          683 t
ORIGIN      
        1 acccggttga tgctttggaa ggcgtcttgt aacccaagct ttcagaaagg ttcaccagca
       61 tttgactggt gatcaccgtg cgcgcggtct gctgcgggat taacgccatc acgacaacgc
      121 ccatcactcc cgtcgcaata atttgccagt aatagcttag cggaaacatg cggacgagat
      181 ggagcgagaa gcggtaaaat aggccggagc ctgtaatcgc ggcgcccaaa cccaaaaccc
      241 cgagagtcat aaaccaagtc gtgctggcga atccgcccat caccgtctgc ggtgattcga
      301 gcttggtaag gatgatgacc atgccgaaga caagcgccac gccatagtcg ggcagtacgc
      361 gaaacaccca aaaaatcagc gtgaagcaaa ttagccccag cagtttagtg gcggcggcgt
      421 ccaggccgat ctccggttgg ccgaaccaaa gataactgcc cacaccaatc cctaaaaccg
      481 cgccgaacca tttactgaac gaaggggagc cgaagatggc gttccaacgc tttacgatgc
      541 tacgggactg cggttttgtt cccgcaggag gcgaaatcgt cggaaaggca ggcaggctga
      601 tttcctgagc acgctcttga taatcattgg aagccgcgct ctcttggctg atcaaattga
      661 gcgcgctacg caattgattg atcgcttgtc cgtagtcatt tcgcttggcg agcatctccg
      721 ccatgcgccg atagccggcg atgccgtcgg cagcgagggc gccggacgcc ttttgggtca
      781 agacttcgcg gtagctgcgg taagcccccg ccagatcgcc taattggatg agagcgctgg
      841 cccgcaagtt gaccaagcgc agatcgggca aaagctgctc gggcggaaac ttatcgagaa
      901 tggtcttgac atacagcggc gcttcgctga gcagatgttc gcttgcggtc aaaagacgga
      961 tcgcattgga ccagtcctga gcttcgacgc ggtgatggac cgcctcacgc agattgccga
     1021 gggcctcata gcgttcagcg aaaaccgcgt gcagccgctg ggtcatctct tcgccctcgg
     1081 ctgcgctcag tttttcgcgc aggaagtctc tgaagaagct gtgcagttcg tatttgccgt
     1141 cttctaaccg gcgaatcagc ggaaactgac tattgccgag ctcgctcacg agatgcgccg
     1201 gctggtcctt tgggaaaagt tcggcccaag tggcttcatc ggccgcaccg agaagcgaag
     1261 cctgtcgaaa gaatgcctgt tcttgcggcg accgcgccga ataaaattcc tcggcgagag
     1321 aattgaacgc ctcgcggccg gtggaatact ggcggtcgac ataggagagc cgcttactga
     1381 gcgtcgcgca gagctgcagc agccaagtcg ggtgcttgtc aatcagttcg tcccaatcct
     1441 cgcgacgcaa ccgcaagagc gaagtctgct tcactgcgcg gatcgtcgct gagcgaggct
     1501 cgcctgagag cagcgccatt tcaccgaacc agtcccgcgc tccgaggatg gcgacgactt
     1561 cagacttgtc cgggccgttt tccaggacca cctgcacgga gcccgattga atcaggtaaa
     1621 atgcatcccc ctgatcgcct tgcgaaatga tgatcgcacc gctctcgaat gaaatctctt
     1681 ccattttgcc gagcactttg gcgacatctt cccgggatag tcccgaaaaa ataaggatac
     1741 tgcgaatggt atcggcaaga gcttccatcg caaaagcaaa ctatcggatc actttatcgg
     1801 ctttctttaa catttcagga gtaaacttga ggtcgagaaa cctggcggtg cgataattga
     1861 gggtgagatc gaattttgcc gctcgttcga tcgctaaagt ctccggcttc tcgcctttga
     1921 cgattttatg aatcaggccc ccggcctggc gtcccatggc gatgaagttc ggaccgtagg
     1981 cggcgagggc gcccccaatg gcccaggttt cctggttgaa catggtcgga aatttttttg
     2041 cccgcgcggt ctcaaaaaga aattctgatt cgctctcgaa cagttcgtcg ggaatttgaa
     2101 aaatcgccgt agccggcatg gcgtgtaagc tggcgaccgt ggttttcaac tcgtccgtgg
     2161 acttgatggc ataaccaact gcctcaaggc tgaatttctt tgctgctgcc tcggcggcgg
     2221 cgaagctgtc gcgcgaaaaa gcattgttgc catcgtagaa gaccaggatc tttcggagtc
     2281 ccgggacgat ttctttaata agctcgaccc gcttttcagt cgtggcgcgg gcgtaggcag
     2341 ccacgccggt gagatttggc ggacgctcgc tgttgctttt gatgaaaccg gcagcgaccg
     2401 gatcgtccgg atgaacaaac acgatgggta tttcctttgt cgcggcgatg accgcccgtg
     2461 tcgcgctggt tcccgtggta aaaatggcgt ttactttgcg cgcgagaatc tctccggctg
     2521 ctggttgcaa aaccgcacga ttacccttga cattacgggt ttcaaaaaac aggttctttt
     2581 tttcctcgta accgagccgt ttgagctctt cgccgaggcc tttgatcgcc tgtgtttgag
     2641 accgttccat ctcctggacc agaatgccca ctcggatcgg tgattgcgcc agaactagaa
     2701 cgggagcagc gagaagcagc gacaacccgg tcacgaataa agcgaaccaa atctttccac
     2761 cactcatgcg agccatcaca atgcgccgtg gctctggccg aaattatcgt aagacaattt
     2821 ccattccctc atgcgccacg acggtggatt gaaaaatctg tttggcgcgt tgttcgatgg
     2881 ttgccatggt cttgtcatca tgagtgggct cgtggtgaaa caatgcgagc tgcttgactc
     2941 cggccttttt tgccacttcg atcgcgagct cgatcgtgct gtgcccccag cctttgcgcg
     3001 gcttggtgcg cgaaaagtat tcgtccgaaa gatactgccc gtcgtggatc agcagatccg
     3061 cgccgcgcgc aaactcgatg acttcggggt aacc
//
LOCUS       DASULZ010000450         5272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_461680, whole genome shotgun sequence.
ACCESSION   DASULZ010000450 DASULZ010000000
VERSION     DASULZ010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5272
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461680"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VFY96_09155"
     CDS             <1..621
                     /locus_tag="VFY96_09155"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606669.1"
                     /translation="IIKHIYEGNGYVMNGPLGANVIGYDPKAKRYPYDPKKAKELLAQ
                     AGFANGVEVRLNFSPDRYPKAREVCQVVADQLAKAGVKTELVSQEFAIFWGSDGVNGG
                     KIPFYYVGRPAIDADTVYDQYYRSGRTKRVGFQHPEVDRLIDEEQKTGDPKKRIAMLQ
                     QIGQILMQEAALVPLYTLAEIYGVARNVIWKAHPEEKVLAFDMKIR"
     gene            660..1502
                     /locus_tag="VFY96_09160"
     CDS             660..1502
                     /locus_tag="VFY96_09160"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606670.1"
                     /translation="MTASDQGIKRRAFLKQGALAGLGAWVGSAWVSSSYAASKERLTI
                     LSSIGLDTLHPYAHSSSPQYGIWNNMIEPLVEVNYAKRDYFGVLAESWQFQGKKWIFK
                     LRKNVRFHDGSPFTANDVIYSINRMKNDKQSLQKENFRDLSEMQALDDNTIAFTTEAP
                     NAVFLDRLQNRFILGKAGMDAQGGDPAEQKPVGTGPYRFVSWQRDGNLVLTRNDNYWG
                     SKAGIKEIVVRRVKEDAGRVAGLLAGQGDIANNIPVEELSRFENNPRVRAEKVEACAC
                     IFWR"
     gene            1484..2194
                     /locus_tag="VFY96_09165"
     CDS             1484..2194
                     /locus_tag="VFY96_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606671.1"
                     /translation="MYFLAMNVTHKPFDNKFLRQAINYAIDPAVIIKYVYEGNGYVMN
                     GPMGSNVIGFDSKIKRYPLDLKKAKELLDKAGYPQGLEIKLYFSPDRYPKAREVCQVI
                     ADQLAKIGIKTDLISQEFVIFWGKDGVNGGKLPFYYVGRPAADADTVYDQYYRSGVSV
                     RTQYKNPEFDKLVDEEQKTGDSKKRVAILQQLGRILMEDVPFVPLYTLAEIYGAARNV
                     VWKPRPDEKIIASEMKIR"
     gene            2573..3091
                     /locus_tag="VFY96_09170"
     CDS             2573..3091
                     /locus_tag="VFY96_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606672.1"
                     /translation="MSQAVPTKMRYSSILRCIGQSLEGMEVKSVDIKSHGDDFIVQAW
                     NRGISMGMDLEKHYTPDDLRKLDAEGRQKRKPFFEPPDLLSLSQILRLAGNYVDRMRG
                     RLLRVSWQDQSDKIQAITVQWQAQPTAEAGAEALAIVEEVCMHIYKQRKKMPLVTERQ
                     AHRPFVSVARGS"
     gene            complement(3103..4746)
                     /locus_tag="VFY96_09175"
     CDS             complement(3103..4746)
                     /locus_tag="VFY96_09175"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX5606673.1"
                     /translation="MALQPLPYPSLILFNGKIRSFDRQGSIHEAIACAGSRIVRTGGS
                     DDMRRLAGPETEVIDLKGRTAIPGLTDTHVHLSEKGTAEMELVDCRDFYVDVGSVADI
                     LQRLANAAASAPKGSWIVAHGSPMQDFRLKDKRFPDKRDLDGAVPDHPVSISFGAHIT
                     VGNTLALAAAKITRDTPDPAGGHIKHDPDTGEPTGELHERAQLILKKVAPEFNYLQLK
                     DGIVFALNQCLERGVTTVHDIVRYAEPVRAYQEIYKEGRMHARVSILPRVIESMIESK
                     SLIELGLITGFGNEWLRVGGVKMSIDGGITGRNACFYEPYEDDEHNHGIIRIQQDELN
                     HTVMKCHEAGLRCCVHAIGDRAFDMALDAYENAIDNSPRKDHRHRIEHMGNWLCTSER
                     MQRMVRSGIVAIPNIAIGYYVGDAILDCVGEKRLTKAFPFRTLLKNNVIIAGGSDSPG
                     YWPVDPLRDIAACVSRKMRWGEVWVPEERISVSDAFAMHTTTASWVGFEENEKGSLET
                     GKLADIAVLAEDPFEIQPEKIKDLKVEMTLVGGEVKYQA"
     gene            4833..>5272
                     /locus_tag="VFY96_09180"
     CDS             4833..>5272
                     /locus_tag="VFY96_09180"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5606674.1"
                     /translation="MAKKPFTWPTNARVAFVLGIAFECWDRSKPSRGGALQSPLPANA
                     LCKRDYSTETFRDFGGKIGVRRLLDICDKYDLKASMPINGLTCEYWRELVKYAHSRGH
                     EMVAHGWDQGEYLYMLTREQERENIFKTVEAITKLTGERPTGWSS"
BASE COUNT         1249 a         1428 c         1487 g         1108 t
ORIGIN      
        1 atcatcaaac atatttacga aggtaacggt tatgtgatga acgggccgtt aggcgccaat
       61 gtgatcggtt acgaccccaa ggccaagcgc tatccctatg atccgaaaaa agccaaggag
      121 ctgcttgcgc aagccggttt cgccaacggt gtcgaggtaa ggcttaattt ctcgcctgac
      181 cgttacccga aagcccgtga ggtttgccag gtagtcgccg atcaactcgc aaaagccggc
      241 gttaaaaccg agctggtctc gcaggaattt gccatcttct ggggatcgga cggcgtcaac
      301 ggcggaaaaa ttcctttcta ctatgtgggc cgtccagcca tcgatgccga caccgtgtac
      361 gatcaatatt accgctcggg aagaaccaag cgggtcggtt ttcaacatcc cgaggtggac
      421 cgtctgatcg acgaggagca aaaaaccggc gatccgaaaa aacggatcgc catgctgcag
      481 cagatcggac agattctgat gcaggaagcg gcgctcgtgc cgctctatac gttggcggaa
      541 atttacggcg tcgcgcgcaa cgtgatctgg aaagcgcacc cggaagaaaa ggtgctcgcc
      601 ttcgacatga aaattcgtta agcgacagca agaagactcg acattgggag gaaattgaca
      661 tgactgcaag cgatcaaggc atcaagcgcc gagcatttct caaacaaggg gcgctcgctg
      721 gactgggcgc atgggttggc agcgcgtggg ttagcagcag ctatgccgcg tccaaggaac
      781 ggctgacgat tctcagcagc atcggcctcg atacgctcca tccctacgcg cacagctcga
      841 gtccgcagta cggcatctgg aacaatatga tcgagccgct ggtcgaggtg aattacgcca
      901 aacgtgatta tttcggtgtg cttgccgaat cgtggcaatt tcagggcaag aagtggattt
      961 tcaaattgcg taaaaacgtt cgatttcacg atggcagtcc cttcaccgcg aacgacgtga
     1021 tttattccat caaccgcatg aagaacgaca agcagagcct gcaaaaggaa aattttcgcg
     1081 acctcagcga aatgcaagct ttggacgata acacgatcgc atttacgacc gaagcgccca
     1141 atgcggtttt cctcgatcgt ttgcagaacc gtttcatcct cggcaaagcc ggtatggatg
     1201 ctcagggcgg agaccccgcc gagcagaaac ccgtcgggac cggcccctac cgcttcgtga
     1261 gctggcagcg cgacggcaac ctggtactta cccgcaacga caattattgg ggcagcaagg
     1321 ctgggatcaa agaaatcgtt gtgcggcgcg tcaaggaaga cgccgggcgt gtggcgggat
     1381 tgctcgcagg ccaaggtgat atcgccaata atattccggt ggaagaacta tcgcgcttcg
     1441 aaaacaatcc gcgcgtgcgg gcggaaaaag tcgaggcgtg cgcatgtatt ttctggcgat
     1501 gaacgtcacg cacaaaccgt ttgataacaa attcctgcgc caggcgatca actacgcgat
     1561 cgatccggcc gtaattatca aatacgtcta tgaaggcaac ggctatgtca tgaacgggcc
     1621 aatgggatcg aatgtgatcg gcttcgattc gaagatcaag cgctatccgc tggatttaaa
     1681 aaaggccaag gagctgctcg ataaagcggg ctatccacag ggactggaaa ttaagttgta
     1741 cttctcgccc gatcgctatc cgaaggctcg cgaagtctgc caagtgatcg ccgatcagct
     1801 cgccaagatc ggcatcaaga ccgacctgat ttctcaggag tttgttattt tctggggtaa
     1861 agacggcgtc aacggcggca agctgccgtt ttactatgtc ggccgaccgg ccgcggatgc
     1921 cgataccgtc tacgatcaat actatcgatc gggtgtcagc gtgcgaaccc agtataagaa
     1981 tccagaattc gacaagctcg tcgacgaaga acagaaaacc ggcgattcga agaaacgggt
     2041 ggcgatcctg caacagttgg ggcgcatctt aatggaagat gtgccgttcg tgccgctcta
     2101 cacactggcg gaaatttacg gtgccgcgcg taacgtagtc tggaagccgc ggccggacga
     2161 aaagattatt gcgagcgaaa tgaagatccg ctaagcgcgg catggcgacg tctgagtcgc
     2221 tgcctccaca ggaaaatgat ggagtaatgg agcactggag cgatggtaaa actccaggct
     2281 tcgcttggtc aatttgtagg ctgtattcac agcattcggg gcgagctcgc ggtggcgagc
     2341 gcgaccggga atccaggatt cagacgccgg tttcgcggag gacggcacaa gcccgggacg
     2401 atttgtcgaa tgggtttcat ctatcggtca ctcacgatta caaccaatct cgctctactt
     2461 ccggacaaaa ttgcacagct cgcacgccga ttattagaaa agggccgatc ttgcaggggt
     2521 tttcgtggca tatcagttgc tcagatgaaa ctccatggaa acgagtactg ctatgagcca
     2581 agccgtgccg acgaagatgc gctattcgtc aatcctcaga tgtatcggac agtcactcga
     2641 aggaatggaa gtaaagtcgg tcgacataaa atctcatggt gacgatttca tcgttcaggc
     2701 atggaatcga ggcatctcca tgggtatgga tctggaaaag cattacacgc cggatgatct
     2761 gcgcaaactc gacgccgaag ggcggcaaaa gcgcaagcca ttttttgaac cgcctgatct
     2821 tctgagtttg tcgcagattc ttcggctcgc cggaaattat gtcgatcgca tgcgcggtcg
     2881 gcttctccgt gtctcgtggc aggatcaatc ggacaagatt caagcgatca ccgtgcaatg
     2941 gcaagcgcag ccaacggctg aggctggagc cgaagcgctc gcgatcgtcg aagaagtatg
     3001 tatgcacatc tacaagcagc gaaagaaaat gccactggtt accgaacgtc aagcacaccg
     3061 tccgttcgtc agtgtcgcac gcggcagctg agtggaaatc tgtcaggctt ggtacttcac
     3121 ctcgccaccc accaaagtca tttcaacctt caaatccttt attttctccg gctgaatttc
     3181 aaacggatct tccgcgagga ctgcgatatc ggcgagcttg ccggtttcga ggctgccttt
     3241 ttcattttcc tcaaagccca cccatgatgc cgtcgtcgtg tgcatggcaa aggcgtcgct
     3301 gacgctgatt ctttcttctg gcacccaaac ctcgccccag cgcatttttc gcgatacgca
     3361 ggcggcaatg tctcgtagcg gatccaccgg ccagtagccc ggcgaatccg agccgccggc
     3421 gatgatcacg ttgtttttca gcaaggtgcg gaacggaaac gctttggtca gccgcttctc
     3481 accgacgcaa tccaaaatcg catcgccgac atagtagccg atcgcaatat tcgggatcgc
     3541 gacgatgccc gagcgcacca tccgctgcat acgctccgag gtgcagagcc agttccccat
     3601 gtgctcgatg cgatggcggt gatccttgcg cggcgaattg tcgatggcgt tttcataagc
     3661 atcgagcgcc atgtcgaacg cgcggtcgcc gatggcgtgc acgcagcagc gtagacccgc
     3721 ctcatgacac ttcatcaccg tgtgattcaa ttcgtcctgc tggatgcgga taataccgtg
     3781 attgtgctcg tcgtcttcgt acggctcgta aaaacaggcg tttcgcccgg tgatgcctcc
     3841 gtcgatgctc attttcacgc cgccaacccg caaccattcg ttgccgaagc cggtaataag
     3901 accgagctcg atgagactct tcgattcgat catcgactcg atgacgcgcg gcagaatgct
     3961 gacccgcgca tgcatgcggc cttccttgta gatctcctga taggcacgca cgggctcggc
     4021 gtagcgcaca atatcgtgga ccgttgtgac gccgcgctcg aggcactggt tcaaagcgaa
     4081 gacgatgccg tccttgagtt ggaggtaatt gaactccggg gccaccttct tgagaatcag
     4141 ctgcgcgcgc tcgtgcagtt cgccggtagg ctcgcccgtg tccggatcgt gtttgatatg
     4201 gccgcccgcc gggtccggtg tgtctcgagt gatttttgcg gctgccaacg caagagtgtt
     4261 gccgaccgtg atgtgcgcgc cgaacgaaat cgataccgga tgatccggaa ccgcgccgtc
     4321 gagatcgcgt ttgtcgggaa agcgtttgtc cttgagacga aaatcttgca tcggactacc
     4381 gtgggccacg atccacgagc ctttcggcgc gctcgcggcg gcattggcta ggcgttgcaa
     4441 aatatctgca accgagccga catcgacgta aaaatcgcgg cagtcgacga gctccatttc
     4501 cgccgtgcct ttttcggaaa gatggacgtg ggtatcggtc agtccgggaa tggctgtgcg
     4561 gcctttaagg tcgatcacct cggtctcggg accggcaagc cggcgcatat catcggaacc
     4621 gccagtgcgg acgatgcgtg aaccggcaca cgcgatcgct tcgtgtatcg atccttgacg
     4681 atcgaagcta cggatttttc cattgaacag aattaaactc gggtacggca aaggctgcag
     4741 tgccatagct gaactcctcc ttgcttggca gaaacacttt ggctataagt cgcaacggat
     4801 cacaaagtca accgtatggg agcgaattag agatggcaaa aaagccgttc acttggccca
     4861 ccaatgcgag agttgcattc gtgctcggta tcgccttcga atgctgggac cgcagcaagc
     4921 cgtcacgcgg cggcgcgctg caatcgccgc tgcccgccaa tgcgttatgc aaacgggact
     4981 attccactga gacctttaga gacttcggcg gcaagatcgg cgtcagacga ttactcgata
     5041 tctgcgataa atacgatctc aaggccagca tgccgatcaa tggactcacc tgcgagtatt
     5101 ggcgggagct ggtaaagtac gcgcatagcc gcggccatga gatggtggcg catggctggg
     5161 accagggcga atatctctat atgctcaccc gcgaacagga gcgcgaaaat attttcaaaa
     5221 ccgtggaagc gatcaccaag ctcacaggcg agcgccccac cggctggtct tc
//
LOCUS       DASULZ010000451         3491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1069311, whole genome shotgun sequence.
ACCESSION   DASULZ010000451 DASULZ010000000
VERSION     DASULZ010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069311"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..512)
                     /locus_tag="VFY96_09185"
     CDS             complement(192..512)
                     /locus_tag="VFY96_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HEX5606675.1"
                     /translation="MDAINETIIEAEVLEALRTCYDPEIPVNIYEMGLIYGTKVDPDG
                     TVTVQMTLTSPSCPAAQALPAEVEEKVKAISGVTDVKVDLVWDPPWDPSKMSEAARLQ
                     LGMM"
     gene            complement(521..1075)
                     /gene="sufT"
                     /locus_tag="VFY96_09190"
     CDS             complement(521..1075)
                     /gene="sufT"
                     /locus_tag="VFY96_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HEX5606676.1"
                     /translation="MSTENPISLRRNCFAIMIPSGERVALSAGATVWLTQALGGSYTV
                     MTERGYSARIDANDSDALGLTAPQSQNSPGRADGSPGSLRDSVWQELRSCFDPEIPVN
                     IVDLGLIYGCDVQSLESGGHKAVVRFTLTAQGCGMGQFLKQDIERKLEKVPGIEQVDV
                     ELVWDPPWNQSMISASAKQQLGIE"
     gene            complement(1102..1548)
                     /locus_tag="VFY96_09195"
     CDS             complement(1102..1548)
                     /locus_tag="VFY96_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEX5606677.1"
                     /translation="MAELDDLYQEVILDHNKSPRNYRVLADANRKAEGYNPLCGDHVT
                     VYLKLNDGVIQDIAFQGSGCAISKASASLMTAELKGKTEAAAEDLFEGVHKMLIGDPE
                     ANHETVGKLKILAGVCQFPARVKCASLAWHTVNAALKGESQPATTE"
     gene            complement(1548..2801)
                     /locus_tag="VFY96_09200"
     CDS             complement(1548..2801)
                     /locus_tag="VFY96_09200"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX5606678.1"
                     /translation="MKAVPHYCASGRVFDVMRLREDFPILRQKVHGKPLVYLDNGATS
                     QKPQAVIDALNHYYTAENSNIHRGIHFLSERATAAYEGAREKVRGFINAESDKEIVYV
                     RGTTEAINLVSQSYGRTFIKVGDEIIVSAMEHHSNIVPWQLLCQQTGAKLRVIPINHK
                     GELVLEEFSQLLNEKTKFVSIAHVSNALGTILPVKEIVRAAHERGIPVLVDGAQAVPH
                     LRVDVRDLGCDFYAFSGHKMFGPTGVGILYGRRELLEAMPPYQAGGDMISLVTFEKTH
                     YNVLPYKFEAGTPNIAGGIGLGAAIDYLLALDWEALAAHEHGLLSYATNALANIDGLR
                     IIGTAAEKAGVLSFVLDSVHAHDVGTILDQEGVAVRAGHHCAMPVMQRFGVPATTRAS
                     FAFYNTTAEIDVLTQAIERVRKVFG"
     gene            complement(2810..>3491)
                     /gene="sufD"
                     /locus_tag="VFY96_09205"
     CDS             complement(2810..>3491)
                     /gene="sufD"
                     /locus_tag="VFY96_09205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEX5606679.1"
                     /translation="SYFCNAVTELTAEPDSLAEHCRVQREGEAGFHTGTFEAKLAQGA
                     HLTAHAVTLAGSLVRNDVHVSLDGEGAECVLNGLYLGSGRQHIDNSTVIEHVKPRASS
                     FELYKGILNGAAHGVFNGKIVVHKDAQKSDARQVNKNLLLSDDAVVNTKPQLEIHADD
                     VKCSHGSTIGQIDPDALFYFRSRGLGLAEARRLLSSAFAADVVARIKVASLRQRLDEY
                     ILEKFRDI"
BASE COUNT          762 a         1028 c          906 g          795 t
ORIGIN      
        1 atcggcgccc aggaatttgg ccgccaattg atattccatc tccttgatac gggcgcgatc
       61 ggcattgcgg aaaaaaaacc cgatcatgcg atcttcgaac actcggtcga taaatgtgtc
      121 gataatcggc ctcagccgac attcgccacc gagcttctcg aacaaactgt tattcatagg
      181 caatctaatc tttacatcat gccgagctgc aagcgagcag cttcagacat tttgctgggg
      241 tcccagggcg gatcccacac gagatcaacc ttgacgtccg taacgccaga tatcgcttta
      301 actttctctt caacctcggc gggcaaggct tgcgctgccg ggcagctcgg ggacgtcagt
      361 gtcatctgaa ccgttaccgt gccgtcggga tcgactttgg tgccgtagat taaacccatt
      421 tcgtagatgt tgaccggaat ttccggatcg taacaggtgc gaagagcttc aaggacctcg
      481 gcttcgataa tagtctcatt aatcgcatcc atgacaaacc tcattcgatg cccaattgtt
      541 gtttcgcgct cgccgagatc atgctctggt tccatggtgg atcccagacg agctcgacat
      601 caacttgctc tatgccggga accttctcta attttctttc gatatcctgt ttgaggaact
      661 gccccatgcc gcaaccctgt gcggttaaag taaaccgcac gacagccttg tgaccgccgc
      721 tttccagcga ttgcacatca cagccgtaga ttaaacccaa atcaacaatg ttgacgggaa
      781 tctccggatc gaagcaggag cgcaattctt gccaaaccga gtcgcggagc gaaccgggac
      841 tgccatcggc acgtcctgga ctgttttggc tctgcggcgc ggtgagcccg agcgcgtcgc
      901 tatcgttggc atcgatgcgt gcactgtatc cgcgttccgt catgactgta taactgcccc
      961 cgagggcctg ggtcagccac acggtagccc cggccgaaag ggcgacccgt tcaccgctcg
     1021 gaatcataat cgcgaaacag ttgcgccgga ggctgatcgg attttctgtg ctcatcgata
     1081 atcctccttt gcaggcggca tttactccgt cgtcgctggt tggctctcgc ccttcagcgc
     1141 cgcgttcacc gtatgccagg ccaggcttgc gcatttgact cgcgcaggaa attgacacac
     1201 gccagcgaga attttaagct tgcccactgt ttcatgattg gcctccggat cgccgataag
     1261 cattttgtga acgccttcaa atagatcctc cgcagccgct tccgttttgc ccttcaattc
     1321 cgccgtcatc agcgaagccg acgcttttga aatcgcgcaa cccgaacctt gaaaagcgat
     1381 gtcctgaatc acaccgtcat tgagctttaa gtagaccgtc acatgatcgc cgcagagcgg
     1441 attgtaacct tccgccttgc ggttggcgtc ggccaaaacc cgatagtttc gcggactttt
     1501 gttgtggtcc aggataactt cctgatagag atcatctagc tcagccatca gccgaacacc
     1561 ttccgaactc gctcgatcgc ttgcgtcagc acgtcgatct cagcggtggt attgtaaaac
     1621 gcaaatgatg cgcgggtagt cgccggcacg ccgaagcgct gcattaccgg catggcgcaa
     1681 tgatgaccgg ctcggacggc gactccctct tggtcgagaa tcgttccgac atcgtgagcg
     1741 tgcacggaat cgagaacaaa cgacagcacg cctgcctttt ccgcagcggt cccgataata
     1801 cgtaatccgt caatattcgc gagcgcgttc gttgcatagc tcaaaagccc atgttcgtgt
     1861 gccgccagag cttcccaatc gagcgccaag agataatcga tggccgctcc taagccgatg
     1921 ccgccggcaa tattcggcgt acccgcttcg aacttgtatg gcagcacgtt gtagtgagtc
     1981 ttttcgaaag tcacgagact gatcatatcg ccgcccgcct gatagggcgg catcgcttcg
     2041 aggagctcac gacggccgta caaaatgccg acgccggttg gcccgaacat tttgtgaccc
     2101 gagaaggcat aaaaatcgca gccaaggtcc cgtacatcga cccgtaaatg cggcacggcc
     2161 tgcgcgccgt caaccagcac gggaatgccg cgctcatgcg ctgctctgac gatctctttc
     2221 accggcaaga ttgtgccgag cgcgttcgac acatgcgcaa tcgagacaaa cttggtcttc
     2281 tcattcagaa gctgcgaaaa ttcttccagg acaagctcgc ccttgtggtt gatgggaatc
     2341 actcgcagct ttgcgccggt ttgctggcag agcagttgcc agggaacgat attcgagtgg
     2401 tgctccatgg ccgaaacaat gatctcgtct ccgaccttaa tgaacgttcg gccgtagctt
     2461 tgtgagacca gattaatggc ctcggtcgtg ccgcggacgt aaacgatctc cttatccgat
     2521 tcggcgttga taaatccccg gactttttcg cgcgcgccct cgtaagccgc agtggcgcgc
     2581 tcgctcaaga agtggatgcc gcggtgaata ttggaattct ccgcggtgta ataatgattc
     2641 agagcgtcga tcaccgcctg aggcttctga ctggtcgcgc cgttatccaa gtaaaccagc
     2701 ggcttgccat gaaccttctg ccgcaagatg ggaaaatcct cccgcaggcg catgacatcg
     2761 aaaacccggc cagaagcaca ataatgcgga acagctttca tcggcttagt caaatgtcgc
     2821 gaaatttctc cagaatatat tcgtccagcc gctgccgtag agacgccact ttgattcgcg
     2881 ccaccacgtc ggcggcaaac gccgagctca aaagccggcg cgcctcggcc aatccgagcc
     2941 cccgggaacg gaaatagaaa agcgcatccg gatcgatctg gccgatggtg gatccatgac
     3001 tgcatttcac gtcatcggca tgaatttcca actgaggctt ggtgttcacc accgcgtcat
     3061 cggataacag caaattcttg ttcacctggc gcgcgtcaga tttttgcgcg tccttatgca
     3121 cgacaatttt gccgttaaag acgccatgcg ctgccccgtt tagaattccc ttgtagagct
     3181 cgaagctgct cgcccgcggc ttcacatgtt cgataacggt cgaattgtcg atgtgctgtc
     3241 ggcccgagcc gagatagagt ccgttaagaa cgcattcggc tccttcgccg tcgagcgaca
     3301 cgtgaacgtc gtttctgacc agtgacccgg caagcgttac cgcatgcgcc gtaaggtgcg
     3361 ctccttgtgc gagctttgcc tcgaaggtcc ctgtgtgaaa acccgcttct ccctcgcgct
     3421 gcacacgaca gtgttcggcg agggaatccg gctcggcggt cagttcagta accgcattgc
     3481 aaaaatagct t
//
LOCUS       DASULZ010000452         3278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_765796, whole genome shotgun sequence.
ACCESSION   DASULZ010000452 DASULZ010000000
VERSION     DASULZ010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765796"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..1296
                     /locus_tag="VFY96_09210"
     CDS             223..1296
                     /locus_tag="VFY96_09210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX5606680.1"
                     /translation="MGSDFVCNQEIIQMARRNLTDNVWHYLTGGAESETTMRRNRYGL
                     DSIAFRPRILVNVSQIDASATLLGHKLRIPVVLAPIGSLQSITPSGGVAVAKAAEQFG
                     TLNLVSSVTEPSLEEIAASSRNPKIFQLYVQGDMKWVENLLTRVRRAGYSALCLTVDT
                     AHYGHRERQMMDRWLPPSRRGAGYEYRAGLTWKTLDTIKEMAGLPFILKGVATTEDAA
                     IAIDHGVSVIYVSNHGGRQLDHGRATIDILPEIVEVAGNKAEIILDGGILRGSDVIKA
                     IALGARAVAIGKLQGWGLAAGGEAGLLSVLEILESEIVTTMGLLGITRLDQLTSSYLC
                     KVQPLSPVHEMSTFPHLLGGQLL"
     gene            complement(1393..1785)
                     /locus_tag="VFY96_09215"
     CDS             complement(1393..1785)
                     /locus_tag="VFY96_09215"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5606681.1"
                     /translation="MAIRKINRTEVEPKKLPSRFFQELVSHKILKSKGIALRFVELLP
                     VREAGARHPHSHRAMEEVIFVQNGTGKAWVNGEVEKIVTGDTILIPAGTRHMMINTGR
                     KPMILICAFSAADPENRYKEHPEISYSG"
     gene            complement(1785..2825)
                     /locus_tag="VFY96_09220"
     CDS             complement(1785..2825)
                     /locus_tag="VFY96_09220"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5606682.1"
                     /translation="MVNDPVRVAAVGLGRWAKVLADAIKRSDRLRLVNCFSRSEKSRD
                     AFAQQYGCRTAKSYEELLADPEVEGLIITTPNDAHAEPIIKAAEKGKDVYVDKPLAHR
                     LADAIAIDHTCREAGIILSVGHSARRLQGSRKIKQIMDRGGIGKLVMAECNFSNDRSL
                     ELTPEKWRWYRDRNPGGPLIQLAVHHTDTLHYLFGPAKSVTCMAKRLYTPAESEDVTM
                     TIVEMESGQLCYIGSNWASQGTFFTYVYGMDANLYFTVDFNFWSSSDVVDRHSQLEIQ
                     HRGSSEHKPIGLESGDMFREQLEEFGDCIRSRKKPEVGGPEGIAALAVIDAALRSAEQ
                     GHTVAIKEVMEG"
     gene            complement(2815..>3278)
                     /locus_tag="VFY96_09225"
     CDS             complement(2815..>3278)
                     /locus_tag="VFY96_09225"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX5606683.1"
                     /translation="QHGAKRVVFLNWHEGNTASMNIAGVSVQQRYGLRVLVVQACYIA
                     LELFGKECGLTHGGELEALPVLAYDPSLVHLERATNSSSPEHARKIDALRRSRAGYPF
                     LKDIRDIAPTGWYGEPQKATPERAKTFLEGVASAAAEYIEKTFKELSEDGK"
BASE COUNT          708 a          914 c          896 g          760 t
ORIGIN      
        1 gcaaaaggat ctataggtac tggtatttgg accggtttgg cgatcttcgc ttacaatgtg
       61 tggcagcgaa cataaccgat taagatttcg aaacgggtgt ttcatcccaa agcaaattgt
      121 gtcgacgatt accgtaaaaa ctcatcctaa atcgtacttt ttcagtgaga actaactaac
      181 gcaaagtgcg caacttcacg ttggcagctc aggaggggaa caatgggctc ggactttgtc
      241 tgcaaccaag aaattatcca aatggcgcga cgcaatctta cggataatgt ttggcactat
      301 ctcaccggcg gcgcagaatc cgaaacaacc atgcggcgaa atcgttatgg tctcgactcc
      361 atcgcgtttc gtccgcgcat actcgtcaat gtttcccaaa tcgatgcgtc ggcgactttg
      421 ctcggccata aactaaggat tccggtcgtg ctcgcgccga tcggatcgct gcagtcaatc
      481 acgccatccg gcggcgttgc cgtggcaaaa gcggccgaac aatttggcac gcttaatctc
      541 gtgagctcag taaccgagcc aagcttagaa gagatcgccg catcgagccg aaatccaaaa
      601 atctttcagc tttacgtgca aggcgatatg aaatgggttg aaaatctctt gacgcgcgtc
      661 aggcgtgccg gctactcagc gctctgcctc accgtcgaca cggcacatta tggtcaccgc
      721 gaacgacaga tgatggatcg ctggctgccc ccgagccggc gcggcgctgg ctatgaatac
      781 cgcgctgggc tcacctggaa gactctcgac acgatcaaag aaatggcagg tctgccattt
      841 atcttaaaag gcgtcgctac aacggaagat gctgcaatcg ccattgatca cggcgtgagc
      901 gtgatttatg tctccaatca tggcggtcgc caactcgacc acgggcgcgc gactatcgac
      961 atattgcctg aaatcgtgga agtcgccggt aacaaagccg agatcatcct cgatggcggg
     1021 atcttgcgcg gcagtgatgt gataaaagcg atcgccctcg gcgcccgggc ggtcgcgatc
     1081 ggcaaattac agggctgggg acttgcggca ggcggcgaag ctggtttgct gagcgttcta
     1141 gaaatcttag aaagcgaaat cgttaccaca atggggttgc ttgggatcac gcgactcgat
     1201 cagctcactt catcctactt atgcaaggtg cagccactga gtcccgtgca cgagatgagc
     1261 acattcccgc acttgcttgg cggacaactg ctctagcgct tgttgcgtcg tgagaccttc
     1321 ctacgagaac gtcagcaggt attcgcaggg gaaggatcag ctcgccaagt caaagaacga
     1381 actcttcggg aactatccag aatagctaat ttcggggtgt tctttatagc ggttttccgg
     1441 atcggcggcg gaaaatgcgc agatcaagat catcggtttg cggccggtgt tgatcatcat
     1501 gtggcgcgtg ccggcgggaa tgagaatggt gtcaccggta acaatcttct caacttctcc
     1561 gttgacccac gccttgccag tgccgttttg cacgaagatg acttcttcca tggctcggtg
     1621 gctgtgtggg tggcgagccc ccgcttctcg aaccggcaac agttccacga accggagagc
     1681 gatgcctttc gatttgagaa tcttgtgcga aactaactct tgaaaaaaac ggctcggcag
     1741 cttcttgggc tcgacctcag ttcgattgat tttgcggatc gccactatcc ctccatgact
     1801 tctttgatcg ccacagtatg gccctgctcg gccgagcgga gcgccgcatc gatcaccgcg
     1861 agagcagcga tgccttccgg gccaccgact tccggttttt ttcggctgcg gatgcaatct
     1921 ccaaattctt ccagctgctc gcggaacata tctccgcttt ccagaccaat cggcttgtgc
     1981 tcggaagagc cgcgatgttg gatctccagt tggctatgcc ggtcgaccac gtcggagctg
     2041 ctccagaaat taaaatccac tgtgaagtag aggttcgcat ccattccgta gacgtaggtg
     2101 aagaaagttc cctgggaagc ccaattggaa ccgatatagc agagctggcc gctctccatt
     2161 tcgacgatgg tcatcgtcac atcttcgctt tcggccggcg tgtaaagcct tttggccatg
     2221 caggtgacgc ttttcgccgg accaaatagg taatgcaacg tgtcggtatg gtgcacggcg
     2281 agttggatca gcgggccgcc cgggttccgg tctctgtacc agcgccattt ttcaggagtc
     2341 aactccaagc ttcggtcgtt ggaaaaattg cattctgcca tcacgagctt gccgatgccg
     2401 cctcgatcca tgatctgctt gattttgcgg ctgccttgta gacgccgcgc gctgtggccc
     2461 accgataaga tgatgccggc ctcacggcag gtgtggtcga tggcgatcgc atcggccaaa
     2521 cgatgggcga gcggcttgtc gacatagacg tcttttcctt tctcggccgc tttgatgatc
     2581 ggctctgcgt gagcgtcatt tggcgtggtg atgatcagcc cctccacttc agggtcggca
     2641 agcagttctt catagctctt cgctgtccgg cagccatatt gttgcgcgaa tgcgtcgcgg
     2701 ctcttttccg agcggctgaa gcagttgacg agacggagcc gatcgctgcg tttgatcgca
     2761 tcggcaagaa ctttcgccca tcgtccgagg ccaacggcag ctactcgcac cggatcattt
     2821 accatcttca ctcaactcct tgaaagtttt ctcaatgtat tctgcagcgg cgctggctac
     2881 gccttctaaa aatgtcttcg ccctttccgg agtcgccttt tgcggctcgc cgtaccagcc
     2941 ggtcggagca atgtctctga tgtcttttaa gaatggatag cccgcgcggc tgcgcctcag
     3001 cgcatcgatc ttgcgcgcat gctccggaga ggaggaattg gttgcccgct cgaggtgaac
     3061 cagagatgga tcgtaagcga gcaccggcaa ggcttctaac tccccgccgt gagtgaggcc
     3121 gcactctttg ccgaaaagct ctaaggcgat atagcaagcc tgcaccacga gcacacgcaa
     3181 accgtagcgc tgctgcacgg atacgccggc gatattcatc gaagcggtat tcccctcatg
     3241 ccaattgaga aacaccaccc gcttcgcgcc gtgctgcg
//
LOCUS       DASULZ010000453         2419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_258360, whole genome shotgun sequence.
ACCESSION   DASULZ010000453 DASULZ010000000
VERSION     DASULZ010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258360"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VFY96_09230"
     CDS             <1..582
                     /locus_tag="VFY96_09230"
                     /inference="COORDINATES: protein motif:HMM:NF022059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX5606684.1"
                     /translation="LGTEKSPGPKLFCVSGQIERPGLYEVPFGISLRHLLFDLAGGLR
                     PNRKLQAVLMGGAAGAFALEKDLDVTLSFENLSAATLPLGSGAVMVFDDTADMRDVLK
                     RLAHFFADESCGKCYPCQLGTRRQHEILDRAAAGHPLAGDRERLSDLGATMVDASLCG
                     LGQTAPMAVLSAMKLWPEMFDPRRFPPPHGEGE"
     gene            614..1315
                     /locus_tag="VFY96_09235"
     CDS             614..1315
                     /locus_tag="VFY96_09235"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX5606685.1"
                     /translation="MSDVTLTIDGASVTVPAGTTILKAAEKLGRTVPTICYHDHCTAN
                     GLCRICVVEVNGVKPLLPACLSPVSEAMKVSTASTRVERSRRTILEMLASAVDLSDAP
                     EIQKFLEQYHADRGRFPDAERRAAPVIDDNPMYIRDYEKCILCWRCVQVCADDAQFTY
                     AINFKGRGFDTGIGTFFDKPMPETTCVFCGQCVGVCPTGALKPKREALLEQGVSLEEI
                     LQATKGGKKRRRKTP"
     gene            1312..>2419
                     /locus_tag="VFY96_09240"
     CDS             1312..>2419
                     /locus_tag="VFY96_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5606686.1"
                     /translation="MIAADRVVSTTCPYCGVGCNLQLHIKDEYIYKVTSPFDGVVNHG
                     NLCVKGRFGYDFIYNPERVTTPLIRKQAQAPGTRTQAFDREQWREVSWDEALDYAATR
                     LVEIYQRDGADAMAVYCCAKATNEDNYLLQKLFRALFRTNNVDHCTRLCHAGSVVALQ
                     MAIGSSAMSNTAGEVIHSDVFLVTGSNTAETHPIIALQMKAAVEKHGAKLIVVDPRRV
                     EMVNWATLWLPEKPGTDVPVFSAMAHVIIKEKLYNREFIESRTEDFAEFSRSMEKFTP
                     EYAEAISGVDRDLIVQAARMYATAKNAAIYWALGIPEHSHGTDNAMALIHLALLTGHI
                     GRPGTGLNPLRGQNNVQGASDSGAMPWHYPGYQRV"
BASE COUNT          503 a          722 c          694 g          500 t
ORIGIN      
        1 ctcggcacgg aaaaatcgcc tggaccaaaa cttttttgcg tatcgggcca gatcgagcgc
       61 ccgggtctat atgaggtgcc attcgggatc tctctacgcc acttgctttt cgacctcgcc
      121 ggcggcctaa gaccaaaccg caaacttcaa gctgtcctga tgggcggtgc tgcgggcgca
      181 tttgcgctgg aaaaagatct cgatgtgacc ctgtcgttcg agaacctgag cgcggcaact
      241 ttgccacttg gctcaggggc tgtaatggtg ttcgatgata cggcagatat gcgggatgtt
      301 cttaagcggc tcgcgcattt ttttgccgat gaaagctgcg gcaaatgtta tccttgccag
      361 ctcggcacgc gccgccagca cgaaattctc gaccgcgccg cagcgggcca tccgctcgct
      421 ggcgatcgcg agcgcttatc cgatctaggc gcgacgatgg tcgatgcctc cctctgcggc
      481 ttgggtcaga ctgcaccgat ggcagtgttg agcgcgatga agttatggcc ggagatgttc
      541 gacccccgcc gctttcctcc cccgcacggc gagggagaat gaaggagcgg gcgtttcatc
      601 gcaggaagtt gtcatgtctg acgtaactct caccattgac ggggcttccg tcaccgtacc
      661 cgcgggcacg acgattctca aagccgccga gaagctcgga cggaccgttc cgacgatttg
      721 ctatcacgac cattgcacgg cgaacggcct ttgccgcatt tgcgtggtcg aagtcaacgg
      781 tgtcaagccg ctgctgccgg cgtgtttgag cccggtcagc gaagccatga aagtttcgac
      841 cgcgagcacg cgcgtcgagc gcagccggcg aactattctc gaaatgctcg cgtctgccgt
      901 cgatctttcc gacgcccccg agattcaaaa atttctcgag cagtatcatg ccgatcgcgg
      961 gcggtttccc gatgccgaac ggcgcgctgc cccggtcatc gatgataacc cgatgtatat
     1021 ccgcgattat gagaaatgca ttctctgctg gcgctgtgtt caagtttgtg ccgatgacgc
     1081 gcagttcacg tacgcgatca attttaaagg gcgcggcttc gataccggca tcggcacgtt
     1141 tttcgacaag ccgatgccgg aaacaacctg cgtcttctgc ggtcaatgcg tcggcgtctg
     1201 ccccaccggc gcgctcaaac ccaagcgcga agcgttactc gagcaaggcg tttcattgga
     1261 ggaaatcctg caggcaacca aaggcggaaa aaaacgccgg aggaaaacgc catgattgcc
     1321 gccgaccggg tcgtttccac cacctgccct tactgtggcg tcggatgcaa tctccagctc
     1381 cacattaaag acgaatacat ttataaagtg acttccccct tcgacggcgt ggtcaatcac
     1441 ggcaacctct gtgtcaaagg ccgcttcggc tacgatttca tctataaccc ggagcgggtg
     1501 acgacgccgc tgattcgcaa acaagcgcag gcgcccggca cgcgcactca ggccttcgat
     1561 cgcgagcaat ggcgcgaggt ttcctgggac gaggcgctcg actacgccgc aactcggctc
     1621 gtagagatct accagcggga cggcgccgac gccatggcgg tttactgctg cgcgaaggcc
     1681 actaacgagg acaactatct tttgcaaaaa ctttttcgcg ccctgttccg cactaacaat
     1741 gtcgatcact gcactcgcct ttgccatgcg ggatcggtgg tggcattgca aatggcaatc
     1801 ggctcatcgg ccatgagcaa cactgccggc gaagtgattc acagcgacgt tttcctcgtg
     1861 accggctcca acaccgccga gacgcatccg atcatcgcgc tgcaaatgaa agccgccgtc
     1921 gagaagcacg gcgcaaaact cattgtggta gatccccgac gggtggagat ggtgaattgg
     1981 gcgacgcttt ggctgccgga gaagccgggg actgatgtgc cggtcttttc cgccatggcg
     2041 catgtgatca tcaaggaaaa actctacaac cgggaattca tcgaaagccg cacagaagat
     2101 ttcgctgagt tctccaggtc catggaaaaa tttacgcccg agtatgccga ggcgatttcc
     2161 ggcgtggatc gtgatttgat cgttcaggcc gcgcgcatgt atgcgacagc caagaacgcg
     2221 gcgatttact gggctctcgg cattccggag cactcccacg gcaccgataa cgcgatggcg
     2281 ctcatccact tggcgcttct tacgggacac atcggtcgcc caggaactgg actcaatccg
     2341 ctgcgcggcc agaataacgt gcagggcgcg tccgactccg gtgcgatgcc ctggcattat
     2401 cccggttatc agcgcgtcg
//
LOCUS       DASULZ010000454         2414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_969205, whole genome shotgun sequence.
ACCESSION   DASULZ010000454 DASULZ010000000
VERSION     DASULZ010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969205"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /gene="lpxK"
                     /locus_tag="VFY96_09245"
     CDS             complement(<1..654)
                     /gene="lpxK"
                     /locus_tag="VFY96_09245"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX5606687.1"
                     /translation="MKLSIRNVWERKGVVGKCVWLLFLPLSLLYMIGAQFRNGMFALG
                     CAKSVRLPKPVLSVGNLTVGGTGKTPSCLWLAHALRDRGIRSGILSRGYGRRKDGAMV
                     LEPSDESVMARSAVSSGDEPLMMAALYSQTVAVAEDRSKAALELLKKKEVDVFILDDG
                     FQHRRVKRDIDVLLLGADTSGWMLPAGPFREPRRNYQRADFLLITGANDKWRSMLRGS
                     "
     gene            complement(694..1956)
                     /locus_tag="VFY96_09250"
     CDS             complement(694..1956)
                     /locus_tag="VFY96_09250"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HEX5606688.1"
                     /translation="MSYVVYNVLLTIGFVLAAPFFPFIWLLGPRYRDGFAQRLGCYGK
                     TVTSGLRDPIWVHAASVGEVRAAGALLRALKAEAPERSVVISTFTATGNRVARQAGGA
                     DKIIFFPLDFSWVARRAVKRINPSLLLFLETEIWPNLVREAYRRGIPTIMLSGRLSAK
                     ALSRYKLARPLFRRVLAQYAALGMQSAEDAARILNLGAPEKKVSVVGSLKFSVDGAVR
                     GSQNVTRSTLSDRQVFVAGSSHRGEELVLLRALQLARARMPRLMMVLAPRHPERFSEV
                     EKLLRESSLSFERKTRLQPDQYFQKDVLLLDTIGDLTEFFNAADVAFVGGSLVDAGGH
                     NLLEPARCRKPVLFGPYMGNFKTIAQGMKRNGGAIEVHGAEDLARVLVELLADAEARQ
                     RIGQSAADFAAAHQEALTKNLSLARQYL"
     gene            complement(1972..>2414)
                     /locus_tag="VFY96_09255"
     CDS             complement(1972..>2414)
                     /locus_tag="VFY96_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5606689.1"
                     /translation="DAVVRAAKAAHAHDFIMAMPQGYDSFVGELGMQLSGGQRQRLAI
                     ARALLKNAPILILDEATSALDTDSERLVQDALENLMADRTTVVIAHRLSTVRRADRII
                     VLVDGTIAEDGTHEELLARNGEYNRLYMTQALEEHDGVEQGVLQ"
BASE COUNT          546 a          731 c          648 g          489 t
ORIGIN      
        1 gctgcctcga agcattgacc tccatttatc gttagctccg gtgattaaaa ggaagtcggc
       61 gcgttgataa tttcgtcgcg gctcacgaaa tgggcccgcc ggcagcatcc agccggaggt
      121 atctgccccc aataacagca cgtcgatatc ccgtttgacg cgccggtgtt gaaacccgtc
      181 atcgagaata aagacatcaa cttctttttt cttcagcagc tctaacgcgg ccttggaacg
      241 atcttcggcg acggcgacgg tctggctgta caacgccgcc atcatcagcg gttcatcacc
      301 ggagcttacg gcggaccgcg ccatgacact ctcatcactc ggctcaagca ccattgctcc
      361 gtccttgcgc cggccgtagc cgcggctcaa tattccgctc cgaattcccc tgtccctgag
      421 cgcgtgcgcg agccacaaac agctcggtgt cttacccgtg ccaccgacgg tgagattacc
      481 gacactcagc accggttttg gcaatcgcac ggatttggca cagccaagcg caaacatgcc
      541 gttacggaac tgagcgccga tcatgtataa caacgatagc gggaggaaca gcagccatac
      601 acatttcccg accacgcctt tgcgttccca gacattccga attgagagct tcattgcttc
      661 ttgcacagtt ctgggtgacc ctaagacgcg agtttacagg tactgtctgg cgagagacag
      721 atttttcgtg agcgcttcct gatgagctgc ggcgaaatcc gccgcgcttt gcccgatccg
      781 ctggcgcgcc tcggcgtcag ctagtagctc taccaaaacg cgcgccaggt cttctgcgcc
      841 gtgcacctcg atcgcgccac cgttacgctt catcccttgg gcaatcgttt tgaagttccc
      901 catgtaaggc ccaaaaagaa ccggctttcg gcaacgggcc ggctcgagaa gattgtgacc
      961 gccggcatcg accagacttc cgccgacaaa agctacgtca gccgcattga agaattccgt
     1021 caaatcaccg atcgtatcga gcagcaaaac atctttttga aaatattgat ccggttgcaa
     1081 gcgcgtctta cgctcgaaac tgagagaaga ctctctcaac agtttttcca cctcggagaa
     1141 ccgttccgga tggcgcggcg ccaaaaccat cataagtctc ggcatcctgg cgcgagccag
     1201 ttgcaaggcc cggagcaaaa ctaattcctc gccacgatgg gagcttccgg cgacaaaaac
     1261 ctgtcgatct gaaagagtgg aacgagtgac gttttggctt cccctgactg cgccgtccac
     1321 agagaacttc aagctaccga cgacagaaac ctttttttcc ggcgcgccga ggttaagtat
     1381 gcgtgccgca tcctccgccg actgcatgcc gagagcggca tactgtgcga gcacacggcg
     1441 aaacaacggc cgcgcgagct tgtaacgcga cagagctttg gcggaaagtc ttccgcttaa
     1501 catgatcgtc gggatgccac gtcggtacgc ttcgcggacg agattcggcc agatttcggt
     1561 ttcaagaaag agcaacaacg acggattaat gcgcttcact gcgcgccgcg ccacccatga
     1621 aaaatccagc ggaaaaaaaa taatcttgtc ggcgcccccg gcttggcgtg cgacccgatt
     1681 gccggtagca gtgaaggtcg agatcaccac actccgctcg ggcgcttcag ctttaagggc
     1741 gcgaagcagc gcacccgccg cgcgtacctc gcccaccgat gcagcatgaa cccagatggg
     1801 atcgcgcagc ccggaagtga cggtctttcc gtagcagccg aggcgttgag cgaaaccatc
     1861 ccggtaacgt gggccgagga gccaaataaa tggaaaaaaa ggggccgcaa gcacaaagcc
     1921 gatggtcaga aggacattgt acacgacgta gctcaccgaa aacttcgatc tttactgcag
     1981 cacgccttgt tccacgccgt cgtgttcctc cagggcttgg gtcatataga gacgattgta
     2041 ttcgccattc ctagcgagca gttcttcatg ggttccgtcc tcggcgatgg tgccgtccac
     2101 caatacgatg atgcggtctg ctcgacgcac cgtagagagg cgatgtgcga tcaccacggt
     2161 ggtacggtcg gccatgagat tttccagcgc atcctgcacg aggcgctccg aatcggtgtc
     2221 cagggcggag gtggcctcat cgaggatcaa aatcggtgcg ttctttagca aagcccgcgc
     2281 aatggccaag cgctgccgtt ggccgcctga cagttgcatc cccagttctc ccacgaaaga
     2341 gtcatagcct tgtggcatag ccatgatgaa atcgtgcgca tgggcggctt tggccgccct
     2401 tacaacagca tcca
//
LOCUS       DASULZ010000455         9483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_665085, whole genome shotgun sequence.
ACCESSION   DASULZ010000455 DASULZ010000000
VERSION     DASULZ010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9483
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665085"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VFY96_09260"
     CDS             complement(<1..301)
                     /locus_tag="VFY96_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606690.1"
                     /translation="MRALLVSGVIFVIATTIGVVDAGYLIRLKNGNDYVTSRYWRDGS
                     QVLFDTYGGVFGVEKNFIAQILKTDDVAGLSSASYREPATNLPDKGLNDKKELRDN"
     gene            1288..1941
                     /locus_tag="VFY96_09265"
     CDS             1288..1941
                     /locus_tag="VFY96_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606691.1"
                     /translation="MARLSFINQSGVSLIEQLVSLLLGSVLITSLYTYFRAELYRTAS
                     VETKTGSLEDARGALDIIIRDLMNAGSWQNGSPPLETGAADDPDQDSDNVCNRVYAAS
                     PSTIHIQMDLNGNGNCADLDPRENIRYELVNPTATCPGPQIIRRNGDCLVANVVPASP
                     GKVFTYYDANGADLGPNPARDAIKRVKIAFSVQLKNPDPNGSGNLTSTVLSSVEFRN"
     gene            1991..3115
                     /locus_tag="VFY96_09270"
     CDS             1991..3115
                     /locus_tag="VFY96_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606692.1"
                     /translation="MVLASCLTILSVLLALGIGIRVMLQNDYRILANLRSSTHSFYYS
                     VAGIEWSKNEIAELDIFPPAPANQMKSFANGTFDVKFSSAAVTGPLTARLTVRSTGTV
                     ANATHVIEAQLTKAYELSDAALAVRGNPARAVLSDGEILISGTDHDQTNGTPRSGAKP
                     RLAISASSEILRGLLSQSIENPEILDGASLAPLVGQSDYLPASFINQLAGDVCSVTTA
                     LLHPISTTGSLTVGNQIWGSQSSPEIHCVDGLSASGDSINITGNVSGAGILVVRNADL
                     VLSGSFHWQGLVILNGQEVGLRTVGSSIKEIVGAVVLNETGAPPSTTAIMEIQGNLRL
                     LFSREALKTAAELIPVTTLTSGYGNLPSLVTQNYWRNVAQ"
     gene            3293..3676
                     /locus_tag="VFY96_09275"
     CDS             3293..3676
                     /locus_tag="VFY96_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5606693.1"
                     /translation="MDTKKTIMVVDDNPDIITIVKTILEGRGYTVFSASSGPELLNML
                     PNQKPDLIILDIMMPEMDGLEVLTRLKGKTETASIPVILLTAKVQYEDVLGGYKLGAD
                     YYITKPFTSTQLVNGINLLLGEGKA"
     gene            3755..4402
                     /gene="tmk"
                     /locus_tag="VFY96_09280"
     CDS             3755..4402
                     /gene="tmk"
                     /locus_tag="VFY96_09280"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX5606694.1"
                     /translation="MGQFITFEGGEGAGKTTQIRLLQKYFQQLNVPYVATREPGDTAL
                     GGLIRKILLEVSVEAVAPLTEVFLYLADRSHHVSQIILPAISAGKIVLCDRFTDSTLA
                     YQGYGRGIDLAWLRELNNTATAFTRPHLTFLLDCPVELGLARTGGRKYEAGKSREDRF
                     EQETIEFHEKVRAGFLALAREEPERFCIIDATKTVEETAADIREIVHRKLFNEPN"
     gene            4389..5375
                     /gene="holB"
                     /locus_tag="VFY96_09285"
     CDS             4389..5375
                     /gene="holB"
                     /locus_tag="VFY96_09285"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEX5606695.1"
                     /translation="MSLTEIIGHTKQLAMLQSALSVERLHHAYFFVGPDGIGKRTAAI
                     ALAKAIHCEKSTNDFCGTCVNCARISDGNDPDVRLIQPLPDKKEISIQQIRELERELN
                     YRSFSGKRKIALIDPATLMNLSAQNALLKTLEEPPDNSLIVLIAPNSGGLLPTVQSRC
                     VRLSFAPLRRHEVAAYLGSQNMIMPDDIDSLAALSMGSIGLAIKLKKEQLFEKRRDWA
                     KILSSLKPGDYHSAMAAAEELGEDRDETLTFLGWAESWFRDLLICQATHRSDELVNLD
                     MLAEIERQASSINGGRLHASMQKVITAPAGIQRNLNRRMILEDLLFTVVAER"
     gene            5375..6907
                     /gene="metG"
                     /locus_tag="VFY96_09290"
     CDS             5375..6907
                     /gene="metG"
                     /locus_tag="VFY96_09290"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX5606696.1"
                     /translation="MERTYITTPLYYVNAEPHLGHTYTTVVADTLKRYFQSIGYEAFL
                     LTGTDEHGDKIAQAAAADGTSPKEYADRISDLFRSTWDACGIAYDHFIRTTDTYHQEY
                     VQKILQKIYDAGEIYFGEYGGLYCYGCERFYTEKELVDGKCPDHQVAPDYISEKNYFF
                     RMSNYQERLIEEIQNKPSLIRPDRYRTEVLAFLREPLEDLCISRPTSRLQWGIPLPFD
                     GNYVTYVWFDALLNYVSALNHRGGDSLQTLWPNAQHLIAKDILKPHAIYWPTMLLASG
                     LPLYDHLNVHGYWVMDSGKMSKSLGNVIRPLEMKARFGMDAFRYFLLREMAFGQDAKF
                     SEEALVTRINADLANNLGNLVSRVLAMQQKYFAGMVQPLGAQWAAEDRALSGKFVQAE
                     NELNSYMAELQFHRALEAIWSALDHANRYIVQTAPFSLIKDPAQKTRVGEVLHHLLEV
                     VRTLARVLAPFMPDTAAELRSLLDIDASILSAPWGKAFSSDHKINPPRVLFPRIETGS
                     GK"
     gene            6921..7691
                     /locus_tag="VFY96_09295"
     CDS             6921..7691
                     /locus_tag="VFY96_09295"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017359325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX5606697.1"
                     /translation="MLIDSHAHIQGKEYNGEVEAVIERARIAGVEKIVAVGGAGDMSS
                     NADAIALAESFHAVFATIGMHPHDAKDVGAEELETLRQLTTHSKVVAVGETGLDYYYD
                     HSPRDVQERVFSSFIHMACETGLPIIVHERDAAEKAAELLYQEGNGKLCGVIHCFTGT
                     YEAARAYLDLGFYLSFTGIITFKNAEALRAVVRQVPLDRLLVETDAPYLTPVPHRGKR
                     NEPAYVRLVAETVANVRGILVEEVARTTSANAQNLFKI"
     gene            complement(7711..8253)
                     /locus_tag="VFY96_09300"
     CDS             complement(7711..8253)
                     /locus_tag="VFY96_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606698.1"
                     /translation="MTARADRTTKIVALLRERVRLEAFHRRLLYISFGILWGSGTSWT
                     LIQWFKDPELRTTRTLLQTFAMKIHGAAMLIFLAVLGTLFTHVRRGWILRANRLSGSF
                     NIGTNVLLALTGWLLYYIADDSARDTASLIHWSIGIAALPLLSAHISLGRATSDRKKN
                     RDEEPAKSPRRARRVDQYWL"
     gene            complement(8250..9203)
                     /locus_tag="VFY96_09305"
     CDS             complement(8250..9203)
                     /locus_tag="VFY96_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019717600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX5606699.1"
                     /translation="MKSRSSEIRRARPLLGTLVEISAAGPEAATGIEAAFREVEAVQR
                     LMSFHNAQSNVSRINSAAPGETLQVNWKTYEVLACAQLLSRLSAGAFDVTVGGKLVEE
                     GFLPRPPRSEACDTNASFEDIVLLPNNSLTLKRRAWIDLGGIAKGYAVDRAVIALKKS
                     GVTSGIVNAGGDLYVLNGAQPIHVRHPEDPSVVLRLGAITNAAVASSSGVFNVRCKSS
                     RDPLVDLRRNDCVTWKQGITVIAPSCMIADALTKVVRLAPVRATTMLSHFSAQGLIID
                     RRGVRIFYGDARERKSTEFLSVAPCHDVRRFLELTRFVNIL"
     gene            complement(9184..>9483)
                     /locus_tag="VFY96_09310"
     CDS             complement(9184..>9483)
                     /locus_tag="VFY96_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEX5606700.1"
                     /translation="ANGGFFFVDEVVGKHEYITYAVGLNSDGTVKQIEIMTYRESYGY
                     EVRNADWRAQFVGKNASAPLKLNQDIKNISGATLSCRHIADGVKRILATYEVALK"
BASE COUNT         2272 a         2414 c         2571 g         2226 t
ORIGIN      
        1 cgttgtcgcg aagctctttt ttgtcgttta gacctttatc tggcaagttg gtagccggct
       61 cgcgataact tgcgctactg agccccgcga cgtcatcggt ttttagaatc tgggcgatga
      121 aatttttctc aacgccgaat acaccaccat aggtgtcgaa caagacctga ctaccatcgc
      181 gccagtagcg acttgtgacg taatcgttac cgtttttcag ccggattagg tatccggcat
      241 cgacaacccc gatcgtcgtg gcgataacga aaatcactcc agaaactaac agggcgcgca
      301 ctgttcctcc taataaaatg tcattttacc atgcctgaga cagatttgcg tattaaatat
      361 tttctctcga cttccagagc ttgacatcgc gccgcgctcg gcagtaatgt ccgcaaacat
      421 ttgcggtccg gtatgggtgt aaacatccga ggattttcca tcgtagaact catcaccgcc
      481 gtggcgttga tagggattgt cgcagccatc gctctgccga gttggatgag tctatctccg
      541 acttattctc taaataactc agtacgacag attcaatccg agttacacag cataaaaatg
      601 cgcgctgccg ctgagggcgt tccatttcag atggcatatt cacagggtgc gaccgcttat
      661 acaattaagc gtgctgcaca gacggttacg acgaagccaa ttgcggaagg aacgacgata
      721 gaaaaagacg gcatgatatc tttttctccg caaggcacgg ctggggctaa ccgagtccgc
      781 ctacgtaatg gcgctggcat atgcaagcaa attgtcgtga gcgcgacagg tcgagtaagg
      841 atctgtgcgc cagtgagttg cgcggaagat tgctagtgta agcgtatgaa gatcctaaac
      901 gcgaacgcgg gcgtgacgtt tgccgaagtc ctcgttgcca tcaatatagc cgtcatcgcc
      961 atcttggcct ctacgctcag cagtataggc atgagccagc ggcaaacgaa tagctcgaat
     1021 tctactgttg cgttccaatt ggctcaggac aagctggaag aattacaagc acgaggaaat
     1081 ctcgcagaga ctaatttctg tccaggtggc ggcgagcatg cccttagccc caaagggggc
     1141 gttattggga tttttgaccg gtgttggcgt gtggccccgt ccactttcgg cggtagtctc
     1201 aaacaaatcg acgtcacagt cgcttggcat gattatgaat cacgggagat tacaatttcc
     1261 acattgcact acagcggaga ctaacctgtg gcgcggttat catttatcaa tcaatccgga
     1321 gtaagtttaa ttgaacaact ggtgtcgttg ctcctcggtt ctgtgctcat cacatctctt
     1381 tacacgtatt ttcgtgcaga actctaccga acggcgtcag tggagaccaa aaccggtagt
     1441 ttggaggatg cgcggggtgc gctggacatc ataatccgcg atctgatgaa tgccggctcc
     1501 tggcaaaatg gatctccgcc gctcgagacc ggagccgcag atgatcccga ccaagattca
     1561 gataatgtat gcaatcgcgt gtatgccgcc agtccgagca caattcatat tcaaatggac
     1621 ctaaacggga acgggaattg cgccgatctc gatccgcgcg aaaacattcg gtacgaactc
     1681 gttaatccaa cggcaacttg tcccggtcct caaatcattc ggcgtaacgg tgactgcctg
     1741 gtcgcgaacg tcgtgcccgc atcgcccggc aaggtattta cgtactacga cgctaatgga
     1801 gcagatctcg gtcccaatcc agcacgggat gcgattaaac gtgtaaagat cgccttcagc
     1861 gtccaactaa aaaatcctga tcccaacggt tcgggtaact tgacttctac ggtactgagc
     1921 agtgtcgagt ttcgcaacta ggcttcgtag gtcgccaaac gcttgcctgg cccagctgaa
     1981 ttctaacggc atggtcctgg cgagctgtct gaccattctc tccgtcctgc tggcactggg
     2041 aatcggcatc agggtcatgc tgcagaacga ttatcgaatt ctggctaatc ttcgcagcag
     2101 cacgcattct ttttattact ctgtggccgg aattgaatgg agcaagaacg agatcgctga
     2161 actcgatatt tttcctcccg cacccgccaa tcagatgaag tcgttcgcga atggcacgtt
     2221 cgatgtcaaa ttctcttcgg ccgcggtgac aggaccgcta accgcacggc taacggtgcg
     2281 ctcaaccgga actgttgcca atgcaacgca tgtcatcgaa gcgcaattaa cgaaggccta
     2341 cgagctttcc gatgccgccc tcgcggttcg ggggaatccc gcgcgggctg ttctcagtga
     2401 tggcgagatc ctgatctccg gcaccgatca cgatcagacg aatggaactc cgaggtccgg
     2461 agccaagcct cgattggcta tttctgcatc cagcgaaata ctccgtggac tgttgtctca
     2521 gtcgatagaa aatccagaga tcctcgacgg agccagccta gcaccgctgg ttgggcagag
     2581 tgattatctt ccggcaagtt tcatcaacca gctggccggc gacgtctgtt ctgttacgac
     2641 ggctttactc cacccaattt ccaccacagg aagtctcacc gttggtaatc agatttgggg
     2701 ttctcaatcg tctccggaaa tccattgtgt cgatgggcta tcagcatcag gagattcaat
     2761 caatattaca ggaaacgtaa gtggggcagg gattttagtg gtaagaaacg ccgatttggt
     2821 tctaagcggc tcgttccatt ggcaaggcct cgtcattcta aacgggcagg aggtcggctt
     2881 aaggacagtc gggtcgagca tcaaagaaat agttggtgca gttgtgctca acgaaacggg
     2941 cgctccgcca agcaccacag caattatgga gattcagggg aacttgcggc tgctatttag
     3001 ccgagaagcg ctcaaaacag cagccgagct gattccagtt acgacgctca ccagcgggta
     3061 cggaaatttg ccatctctgg ttacgcagaa ctattggcgg aacgtcgcac aatagtaaac
     3121 ggttggggcc tactttcaat ttacaaccgc cgttcaagat gctaaattac gaatctacaa
     3181 acactttgag aattaaaacc cagttctttc accgccttac cgaagtctga attccggcgg
     3241 ctttagcgcg cgaacatcgt tcgatgggga aggggcgtga gagggagttc gcatggatac
     3301 aaaaaagacc ataatggtcg ttgacgataa tccggacatt atcactatcg tcaagaccat
     3361 tctggaaggc cgtggctata cggtttttag tgcttcgagt ggccctgagc ttttgaacat
     3421 gctgccaaat caaaaaccgg atctaatcat actggatatc atgatgccgg agatggacgg
     3481 acttgaagtg ctcacccgat tgaagggaaa aaccgaaact gcaagcattc cggttatttt
     3541 actgactgcg aaagtacaat atgaggatgt tcttggcggt tataaactcg gggctgatta
     3601 ttacatcacc aagcccttta ccagcaccca gttggtgaac ggaataaacc tgctgttggg
     3661 cgaaggcaaa gcgtaaacgc ttcctacccc gagattcttt cagcgcgctg ttgcgctttg
     3721 cacctttgcg tgtttttctc cattcttcgg agcaatgggc cagtttatta cgtttgaagg
     3781 aggcgaaggg gccggtaaga ctacccaaat tcgactgctt caaaaatact ttcaacaatt
     3841 gaatgttccc tatgtcgcaa cgagagagcc gggggatacg gcgcttggcg gcctgattcg
     3901 gaagatattg ttggaggtca gcgttgaggc cgttgcgcca ttgaccgagg tgttcttata
     3961 cctcgctgat cgttctcatc acgtttcgca gattatcctt ccggcaataa gcgcagggaa
     4021 gattgtttta tgtgaccgct ttaccgattc gacgcttgct taccaggggt acggtcgagg
     4081 tatcgatcta gcttggttgc gggagttgaa taataccgcc actgcgttca cccgaccgca
     4141 tttaacattt ttgctcgatt gtcctgtcga acttggattg gcccgaacag gcggacgtaa
     4201 atatgaagcg ggcaagtcac gagaagatcg gtttgagcag gagacgatcg agtttcatga
     4261 aaaggtgcgc gccgggtttc tcgctctagc acgagaggag ccagagcgat tttgtattat
     4321 agatgcaacg aaaacggtgg aggaaaccgc agcagacatt cgcgagattg tccaccggaa
     4381 attatttaat gagcctaact gaaattatcg ggcatacaaa gcagcttgca atgctgcaat
     4441 ccgcattatc ggttgagcgg ttgcaccacg cttatttttt tgtcggaccg gatggtattg
     4501 gcaaacgcac agcagctatc gcactcgcca aagcgattca ttgcgaaaaa agcactaatg
     4561 atttttgcgg aacttgcgtc aattgcgcgc ggatcagcga tggcaatgat cccgatgtta
     4621 ggctgattca gccgctgcca gacaagaagg aaatcagcat ccagcaaatc cgcgagcttg
     4681 agcgcgagct aaactatcgc tcttttagcg gcaagcgcaa aatcgcactt attgatccgg
     4741 caacgctgat gaacctgtcg gcacaaaatg cgctgctgaa aactctcgag gaaccgccgg
     4801 acaattcgtt gatcgttttg atcgcgccga actcgggtgg tctgttgccg acggtgcagt
     4861 cgcgttgcgt caggcttagc ttcgcgccgc tgagacgcca tgaggtggcg gcgtacttgg
     4921 gctcgcaaaa tatgataatg cctgatgata tagactctct ggcagcgtta agtatgggga
     4981 gcatcggcct agcgatcaaa cttaaaaaag aacagctgtt cgaaaaacgg agagattggg
     5041 cgaaaattct tagctctctc aaaccaggcg actatcactc cgcaatggcc gctgccgagg
     5101 agcttggcga ggatcgcgat gagacgctaa cttttcttgg ttgggcggag agttggtttc
     5161 gagacctgtt aatttgccaa gcgactcatc ggtcagacga gttggtcaat ctggatatgc
     5221 tggcggaaat cgagcgacaa gcgtcaagca ttaacggcgg tcggcttcac gcatccatgc
     5281 aaaaggtcat caccgcgcct gccggcattc agcgcaacct caaccggcgc atgattttag
     5341 aagatctgtt gttcacggtt gtggcggagc ggtaatggaa cggacttata ttactacgcc
     5401 actctactac gtcaatgcgg aaccgcacct cggccacacg tatacgacgg tcgtggccga
     5461 cactttgaag cgctactttc agtcgatcgg ttacgaagca tttttgctca ccggtactga
     5521 cgagcatggg gacaaaatag ctcaagcggc ggctgccgac ggcaccagtc caaaggagta
     5581 tgcggatcga atcagcgatt tgtttcgttc gacctgggat gcgtgcggca tcgcctacga
     5641 tcacttcatt cgcactactg acacatatca tcaagaatac gtgcaaaaga ttttacaaaa
     5701 gatttacgat gcgggtgaaa tatattttgg cgagtacggc ggactttact gctacggctg
     5761 cgagcgcttt tacaccgaga aagagctagt ggacggcaaa tgccccgatc atcaggtcgc
     5821 accggactac attagcgaga agaattattt ttttcggatg agcaactatc aggagcgttt
     5881 gatcgaggag attcagaaca agccgagtct tattcgaccg gatcgctatc gcacagaagt
     5941 tttggctttt ctccgcgaac ctcttgaaga cctctgtatc tcacggccaa ctagtcggct
     6001 ccaatggggt attccgcttc catttgatgg taactatgtg acttatgtct ggttcgatgc
     6061 attgctcaat tacgtgagcg ctttgaatca tcggggcggc gattcgctgc aaactttatg
     6121 gcccaatgcg caacatctca tcgccaagga cattctcaag ccgcatgcga tctactggcc
     6181 aacaatgctc ctggcctctg gcttgccgct ctacgatcac ttgaacgtgc atggctattg
     6241 ggtgatggat tccggcaaga tgtccaagag cttgggcaac gtcatccgtc cactggagat
     6301 gaaagcgcgg ttcggcatgg atgcttttcg ctattttttg ctgcgcgaaa tggctttcgg
     6361 tcaggacgcg aagttttctg aggaggcatt ggtcacacgt atcaacgccg atctggcgaa
     6421 caacctgggt aatctagtga gccgtgtgct ggcgatgcag cagaagtact tcgccggtat
     6481 ggtgcagccg cttggtgccc agtgggctgc ggaagatcgc gcgctcagtg gcaaatttgt
     6541 gcaggccgag aacgagctaa acagctacat ggcggaactg caattccatc gcgccctcga
     6601 agcaatatgg tccgcgcttg atcatgccaa tcgttacatt gttcagacag cgccattcag
     6661 tttgatcaaa gaccccgccc agaaaacgcg ggtcggcgaa gtgttgcatc atttgctcga
     6721 agtggtgcga acgctggcgc gagtgcttgc gccatttatg ccggatactg ccgccgaact
     6781 ccgttctcta cttgatatcg atgccagcat tttgagtgcg ccctggggaa aggccttcag
     6841 cagcgaccac aaaattaatc cgccaagggt tttgtttccg cgcatcgaga ctggtagcgg
     6901 caaatgatga tcgtgcgatc atgttgatcg acagccacgc tcatatccag ggtaaggaat
     6961 acaatggcga agttgaagcg gtgattgaac gcgcgcgcat agcaggcgtc gaaaagatag
     7021 ttgcagtcgg cggtgccggt gatatgtcga gcaatgcgga tgcaatcgcg ttggccgagt
     7081 cttttcacgc tgtctttgct acgattggca tgcacccgca cgacgcgaaa gatgttggcg
     7141 cagaggagtt ggaaacgctt cgtcagttga ccacgcattc caaagtcgtt gcggtcggtg
     7201 agacggggct cgactactat tatgatcatt ccccgcgcga cgttcaggaa cgggtgttca
     7261 gcagtttcat tcacatggcg tgcgaaacgg gtttgccgat catagttcac gaacgcgatg
     7321 ccgcagagaa agcggcggaa ctgctatacc aggaaggcaa tgggaagctt tgcggggtga
     7381 ttcactgttt taccggcact tatgaggctg cgcgcgctta tctagatcta ggtttttacc
     7441 tttcgttcac tgggattatt acgttcaaaa acgctgaggc gctaagggcg gtggttcgac
     7501 aagtcccttt agaccggctg ttagtcgaaa ccgatgcgcc atatctcacg ccagtaccgc
     7561 atcggggtaa acgcaatgag ccagcttacg tacgacttgt agcggagact gtggcaaatg
     7621 tcagaggcat tttagtggaa gaagtcgcca ggaccaccag cgcgaatgcg cagaatcttt
     7681 tcaaaattta gtggatcacc actcattgat tcacaaccag tattgatcaa ctcttcgcgc
     7741 gcgacgcggt gatttcgctg gctcctcgtc ccgatttttt tttcgatcag atgtagcacg
     7801 gccaagcgag atatgcgctg agagcagggg cagtgcggcg atgccgatgg accaatgaat
     7861 aaggcttgcc gtgtcccgcg ctgaatcgtc agcgatgtag taaagcagcc acccggtgag
     7921 cgccaagaga acgttagtcc cgatgttgaa ggagccggac aatcgattgg ctcgtaaaat
     7981 ccagccacgg cgcacatggg taaacaatgt gccgagaacg gcgaggaaaa tgagcatcgc
     8041 cgcgccatga attttcatgg caaatgtctg aagcagagtt cgagtcgtgc gaagctcggg
     8101 atctttgaac cattgaatga gcgtccacga agtaccgctg ccccacagaa tcccgaagct
     8161 gatataaagc agcctgcgat gaaaagcctc gaggcgcacc cgctcccgca atagcgccac
     8221 gattttggtt gttcgatcgg ctctggcggt cataaaatgt tcacaaaccg tgtgagttcc
     8281 agaaacctgc ggacatcgtg gcagggggcc acactgagga actccgtgct cttccgttca
     8341 cgtgcgtcgc cgtaaaaaat gcgaacgccg cgccggtcga tgatcagccc ctgcgcgcta
     8401 aaatgagaca acattgtcgt cgcgcgcacg ggcgcaagac gcacgacctt ggtcaaagca
     8461 tcggcaatca tgcaactcgg cgcgatcacc gtgatgccct gtttccaggt tacgcaatcg
     8521 tttcgtcgta ggtcaaccag cggatcgcgg ctgcttttgc atcgtacatt gaaaactccg
     8581 ctcgagcttg cgacggccgc attggtgatg gcgcccaggc gcaagacgac agagggatct
     8641 tccgggtggc gaacgtgaat tggctgcgcc ccattgagga cgtagagatc gccgccggcg
     8701 ttgacgatgc cgctggtgac gccggatttt ttgagcgcta tcacggcgcg atccacggcg
     8761 tagcccttgg caattccgcc gaggtcgatc caggcgcggc gcttgagcgt aagcgaattg
     8821 tttggcagca aaacgatatc ttcgaaggat gcgttcgtat cgcaagcttc ggaacgcggc
     8881 ggtcgcggaa ggaagccttc ctcgacaagt ttgccgccga ctgtgacatc gaaggcgccg
     8941 gccgagagtc ggctcagcaa ttgcgcgcaa gcgagaactt cgtaagtttt ccaattgacc
     9001 tgcaaggttt cgccaggcgc cgcgctattg atgcgtgaca cgttgctttg agcattgtga
     9061 aagctcatca agcgctgcac ggcttcgact tcgcgaaagg cggcctcgat cccggtggcg
     9121 gcctccggcc cggccgcgct gatttccacc aacgtgccga gcagcggccg ggcacggcgt
     9181 atttcacttg agcgcgactt cataggtagc caaaattcgc ttcacaccgt cggcgatatg
     9241 gcggcaagac aaagtcgcgc cgctgatatt tttgatgtct tggttaagct tgagcggtgc
     9301 cgaagcgttt ttgccgacaa actgcgcgcg ccagtcggca ttgcgcactt cgtagccata
     9361 actttctcgg taagtcatta tctcaatttg cttaacggtg ccgtcggaat tcagaccgac
     9421 ggcgtaggtg atatactcgt gtttaccgac gacttcatcg acgaaaaaaa aaccgccatt
     9481 agc
//
LOCUS       DASULZ010000456         5156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_462170, whole genome shotgun sequence.
ACCESSION   DASULZ010000456 DASULZ010000000
VERSION     DASULZ010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462170"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VFY96_09315"
     CDS             <1..786
                     /locus_tag="VFY96_09315"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX5606701.1"
                     /translation="EWWLVVRCSFVGVWVGIVPGLGSQVVDWLAYGHAAQTCKGGRQT
                     FGKGDVRGVIAPESANDAKDGGDLVTTLLLGFPQGVVTALFIVALLAWGYLPGPEMIK
                     KNPEVIYSIVWLQGISGIVGTLVGFVLAAQLAKLAEVRYTFMVPIMFIFILLGAFSVN
                     RDPLDLVVVIGFGIVGYFMRRYGYPRPAMILGLVLGDLMEKYLYRSVASYGFSWLTRP
                     AVIALFLMAGASLFFTLRGQMKAKKEAPPSSFTGAMARAEDES"
     gene            776..1243
                     /locus_tag="VFY96_09320"
     CDS             776..1243
                     /locus_tag="VFY96_09320"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX5606702.1"
                     /translation="MKANITLFFLVLFVLAAVLGWDWPNIAKIMPVYVAAIPGIVLTL
                     VQIYREATDWEGRRGGQGGIEMDEVTDVKLEKKVELQRTLSFFAWFIGGALGIWLLGL
                     TISLPLLIFLYALIEGKEKWYTALLMAVCTYGFVWGLFEYVLETRWPPGLLFG"
     gene            1281..2333
                     /locus_tag="VFY96_09325"
     CDS             1281..2333
                     /locus_tag="VFY96_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606703.1"
                     /translation="MNLWKIVCEISCIFASCFAFQLAFAQQPYYQNKTIRIIRGGEPG
                     GSGDLQARALIPFLKKNIPGDPTIIVENMPGAAGRKAVNYIYSTAKPDGLAIGAVGAG
                     LVAGPILGLPGSQYDIDKLIYLGSTESGDPYVFVSRKEAGFDSLEKLRAASGLRIGAQ
                     AVGHPVFISGRMFGYLLGLKDPRMVVGYGGPEIDIALAKGEVDLRANGADTIFQRNRE
                     GLDKASLNFHAYITVPKGKFIPGLPKAPELESFAKNDKEREIVNLFRAFLYPRWPYIV
                     PPGTSKEVVAILRGAMAKAFKDTEFHKEFKKLMSNDPTPLTGEEMESAIRDLPRDPET
                     VALYKKMADQGPLPPR"
     gene            2495..3559
                     /locus_tag="VFY96_09330"
     CDS             2495..3559
                     /locus_tag="VFY96_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606704.1"
                     /translation="MNVNRRRLRVPVEALLIASLLLCSTAAVAQTPFYQGKTLTIIQG
                     TEAGGSSDMMTKAMLTHLKKHIPGEPTIISEYMPGGGGIKAANHIFKNAKPDGFTIGR
                     IGGGLVANAVLGEKGVLYDLNKFIYLGSPHSTYHWVFITRQEANLKTIEALRSTPGIR
                     VGAQAVGHSNYFIGRLFSYLIGFKDPKFVVGYGGTELDVALMRGELDGRINNADTLQT
                     RNADLIAKKAIDIHAIMEVPKGLKQPGFERLPEIEEFAKTEKEHKLLAMIRIFRQIGT
                     PSILPPGTPPEQVKILRQAMAKMFSDPEFHRDYKKFVGEEPTPLSGEEMERAIKELPR
                     DPEIVDLFKNLNAAGPLPPR"
     gene            3936..4766
                     /locus_tag="VFY96_09335"
     CDS             3936..4766
                     /locus_tag="VFY96_09335"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606705.1"
                     /translation="MAFYFGKEKGFFSQEGLEAQLVAMAPPTAIVALVAGELDFSTTL
                     GAATSAMMRGSAIKRVFYVQNEPTFALTAQPEIKSTRELMEKVIGVNAPTDAMGMSAK
                     LILKGNGIDPSRVTFLSTQVTENAYKALLSKRVAATLLPPPFAEEAEAKGYSRLAEAK
                     DYAPLSTIGLVTSVQAVQRKTGTVQAVIRSLLKTMAYLRNSENRREMVQYITGSFKID
                     ASVADKAFASMLTTYSTNGTKPRAAIEREIEIYRETLQVTKLFTPDDLEDMSILKKLQ
                     "
     gene            4884..>5156
                     /locus_tag="VFY96_09340"
     CDS             4884..>5156
                     /locus_tag="VFY96_09340"
                     /inference="COORDINATES: protein motif:HMM:NF020896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HEX5606706.1"
                     /translation="MRLDLGLIHIKNVRFGAETSIDDDILSIDREALVALLEQESLFD
                     RVDVDLAHPGESCRILRVLDVVEPRYRLNGFNFPGALDPNGLVGDGK"
BASE COUNT         1153 a         1375 c         1452 g         1176 t
ORIGIN      
        1 gaatggtggt tggtggttcg ttgcagtttt gtcggcgtct gggtgggcat cgtacctggg
       61 cttggctcgc aggtcgtcga ttggcttgcc tacggtcatg cggcgcagac ctgcaagggc
      121 ggcagacaga ccttcggtaa gggcgacgtg cgcggagtga tcgcgcctga aagcgccaac
      181 gacgctaagg acggcggcga tctcgtgacc accttgctcc tcggatttcc acagggagtg
      241 gtgaccgcgc ttttcatcgt cgcgctgctt gcctggggtt atctgcctgg cccggaaatg
      301 atcaagaaga acccggaggt catctacagc attgtgtggc tccaaggaat ctcagggatc
      361 gtcggcacgc tcgtcggctt tgtattggcc gcccagctcg cgaagctggc cgaggttcga
      421 tacacattca tggtcccgat catgttcatc tttattctcc tgggtgcgtt cagcgtcaac
      481 cgcgatcctt tggatctcgt ggtcgtgatc ggcttcggta tcgtcggcta tttcatgcgc
      541 cgctatggtt atcccaggcc ggcgatgatt ctcggcttgg ttttgggcga tctgatggaa
      601 aagtatctct accgttcggt ggcgagctac ggcttcagtt ggcttacccg cccggcggtc
      661 atcgcgctat tcctgatggc gggcgcatcg ctgtttttta cgctgcgcgg acagatgaaa
      721 gccaaaaagg aagctccgcc ttcttctttc accggagcaa tggcaagggc tgaagatgaa
      781 agctaacatc accttattct ttcttgtgtt gtttgtattg gcggccgtgc tgggttggga
      841 ctggcccaac attgccaaga tcatgcccgt ctatgtcgcc gctattccgg ggatcgttct
      901 aacgttggtg cagatttacc gagaggcaac cgattgggaa gggcggcgcg gcggccaggg
      961 cggaatagaa atggacgaag tgaccgacgt aaagttggaa aagaaggtgg aactacagcg
     1021 caccctgagc tttttcgctt ggttcattgg cggcgcgttg ggcatctggc tgctcggcct
     1081 cacgatcagt ctgccgctgt tgatttttct ctacgcgctg atcgagggca aggagaaatg
     1141 gtacaccgct ttgcttatgg cagtttgcac ctacgggttt gtttggggtc tgtttgagta
     1201 cgtgttggaa accagatggc cgcccgggct tctgttcggc tgacaaccgc tcgatcagtt
     1261 tcgccgggaa gggagaacca atgaatttgt ggaaaatcgt ctgcgaaatt tcctgcatat
     1321 tcgcttcatg ctttgccttt caattggctt ttgctcaaca accctattac cagaataaga
     1381 ccatcaggat catccgtggt ggcgagcccg gcggctcggg cgatctgcaa gcgcgggcgc
     1441 tgattccctt tctgaaaaaa aatattcccg gcgatccgac aatcatcgtc gaaaatatgc
     1501 cgggagccgc cggtcgaaag gcggtaaatt acatttactc gacggccaag cccgacggtc
     1561 tcgcgatcgg cgcagtcggc gcgggtctgg tcgccggtcc gatcctcggg ctgcccggtt
     1621 cgcaatacga tatcgacaag ctcatttatc tcggctcgac ggagagcggc gatccctatg
     1681 tttttgtcag ccgcaaagaa gctggtttcg acagtctgga aaagctgcgc gccgccagcg
     1741 gcttgcgcat cggtgctcag gcggtgggcc atcctgtgtt catcagcggg cgcatgttcg
     1801 gttatcttct aggcttgaaa gatccgcgca tggtggtcgg ctacggcggc cccgaaatcg
     1861 atatcgcatt ggccaaggga gaagtggatt tgcgcgccaa cggcgccgac actatttttc
     1921 agcgcaatcg cgaaggcctc gacaaagcat cgctcaattt tcatgcctac atcacggttc
     1981 ccaagggaaa gttcattccg gggctgccca aggcgccgga gctggagagt tttgccaaga
     2041 acgataagga gcgggagata gtgaatctgt ttcgcgcgtt tctatatccg cgctggccat
     2101 acatcgtgcc gccgggaacg tccaaagaag tggtcgctat cctgcgcggc gcgatggcaa
     2161 aggctttcaa ggatacggag tttcacaagg aattcaaaaa gctcatgagc aacgatccga
     2221 caccgctcac cggcgaagaa atggaaagcg cgattcgcga tctgccgcgc gatcccgaga
     2281 ccgttgcgtt gtacaagaaa atggccgatc agggaccgtt gccgccgcgc taacgcgcat
     2341 gcgttcaaaa ccgttgaagt ggttcaagcc gttcagaacg ttgaaggaag ctcatgttga
     2401 ttcgacatgc ccatgacgag gagtttgtct tgacagatcg atcattcaaa cggttgaacg
     2461 atttaaacct ccgttgacgg aaaaggaggt ttcgatgaat gtgaatagac ggcgcttgcg
     2521 tgttccagtc gaggctctac tgatcgcttc gctgctgctg tgctcaactg ccgccgtcgc
     2581 tcagacgccg ttctatcagg gcaaaacact aacaattatc cagggcaccg aagccggcgg
     2641 cagctcggat atgatgacca aggcgatgct tacgcatcta aaaaagcaca ttcccggcga
     2701 gccgaccata atttccgagt acatgccggg cggcggcggg atcaaagccg ccaaccatat
     2761 tttcaagaat gccaagccgg acggcttcac catcgggcgt atcggcggcg gccttgttgc
     2821 caacgccgtg cttggagaaa agggcgtgct ctacgatctc aacaagttca tttatctggg
     2881 ctcgccgcac agcacctatc actgggtgtt tatcaccagg caggaagcta atctgaaaac
     2941 catcgaagca ctgcgttcga cgcccggaat tcgcgtcggc gcgcaggcgg tgggacattc
     3001 gaattacttc atcggccgct tgttttctta cttaatcggt ttcaaagatc caaagttcgt
     3061 tgtcggctat ggcggcaccg agctcgacgt cgcgctcatg cgcggagaat tggacggccg
     3121 catcaacaac gcggatacgc tgcaaacccg caacgcggac ctcatcgcca aaaaagcgat
     3181 cgacatccat gcgatcatgg aagttccgaa aggtctcaaa cagcccggct tcgaacgctt
     3241 gccggaaatc gaggagttcg ccaaaacgga aaaagaacac aagctgctgg cgatgattcg
     3301 gattttccgg cagatcggca caccgtctat attgccgccg ggcacaccgc ctgaacaagt
     3361 taaaatactc cggcaagcga tggctaaaat gttcagcgat ccggagtttc acagggatta
     3421 caaaaaattc gtcggtgaag aaccgacacc gctctcaggc gaagaaatgg agcgcgcgat
     3481 caaagagttg ccgcgcgatc cggagatcgt cgatcttttc aaaaacctca acgccgccgg
     3541 cccgctgccg ccgcggtaac acttccaagc tttacacgct tcaggctatt acttccgcga
     3601 ggaagatcgg aaggtggtag attatccaac atgaaggcca gcacaaagta cgacccgagc
     3661 aacttttttc acatcaatca gaacatcagt ccgtcgacgt aagctgtgcc cgcttgggca
     3721 aaggctactc gcgcacggag ttcaatatct gaaagattgt cgcctgattc ggaagggggc
     3781 agtttatgtc tttcgcgtat tggctgacat ttctgtgcag atcaactgtt attccaattt
     3841 tgacgatctt tgtgctttct cctgttccta tttgttgggg cgcgggggct ccgaccaaaa
     3901 taaccatcca tgtacctggg aaaactttgg cggtgatggc gttctatttc ggcaaagaga
     3961 agggtttctt ttctcaggaa ggcctcgagg ctcagctcgt agcgatggcg ccgcctacag
     4021 ccattgtcgc tttagtcgct ggagagttgg atttctcgac aacactgggt gctgcaactt
     4081 ccgccatgat gcggggctct gcgatcaaac gcgtttttta tgtccaaaac gagccgacat
     4141 tcgctctgac tgcacaaccg gaaatcaagt cgacccgtga gctgatggaa aaagtcatcg
     4201 gcgtcaatgc gcccaccgac gctatgggga tgtcggcgaa attaattctc aagggcaacg
     4261 gcatcgatcc atctcgagta accttccttt cgactcaggt cactgaaaat gcttacaagg
     4321 cgctgctcag caaacgagtc gcggcgacct tgctcccgcc gccgtttgcc gaggaggccg
     4381 aggcaaaagg ctatagccgc ttggcggaag ccaaggacta cgcgccgctc agcacgattg
     4441 ggcttgtcac ttccgtgcag gccgtgcaaa ggaagaccgg caccgtccag gccgtgattc
     4501 gatcgctgct taagaccatg gcgtacctac gcaattcgga gaatcgccgg gagatggttc
     4561 agtacatcac gggatctttc aagatcgacg cgtccgttgc tgacaaagcg tttgcttcga
     4621 tgttgacgac ctatagcacc aacggcacta agccacgagc agccatcgaa agagaaattg
     4681 aaatctatcg ggaaactctt caagtcacta aattattcac gccggacgac ctcgaggaca
     4741 tgagcattct gaagaagctt caataaggct ctgactaagc gcttcatgac taagctgata
     4801 gttcggcggg tttccgtatc gcgactagta gtgtcactcc tgcgcactac ccagaaacgc
     4861 ggatagatga taataaggcg cctatgcgat tagatctcgg tttgattcac atcaaaaacg
     4921 tccgcttcgg cgcagagacc tcgatcgatg acgacattct ctcgatcgac cgtgaagcac
     4981 tggttgctct gctcgaacaa gagtcgctgt tcgatcgcgt ggacgttgac ctagcgcatc
     5041 ctggcgagtc ctgtcgaatt ctccgcgtgc tcgatgttgt cgagccgaga taccggctga
     5101 acggatttaa ttttcccgga gcgctcgatc ccaatggcct cgtaggcgat ggcaag
//
LOCUS       DASULZ010000457         4539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_359726, whole genome shotgun sequence.
ACCESSION   DASULZ010000457 DASULZ010000000
VERSION     DASULZ010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359726"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VFY96_09345"
     CDS             complement(<1..391)
                     /locus_tag="VFY96_09345"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606707.1"
                     /translation="MHADQFIEELWGYAAEVPMEQHPWFDGIIKHRWTREQIILGEVQ
                     HYLRVRTNPIFFGYMAVNAVSAKDYSVIDTVLENFMEELGGKQTHMDIMLQFLEEGSI
                     SREEADNAEPAPGTLAAIEMIIGCCQRR"
     gene            556..1275
                     /locus_tag="VFY96_09350"
     CDS             556..1275
                     /locus_tag="VFY96_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX5606708.1"
                     /translation="MNLWVGTSGYSYKAWLGSFYPERLPAKEMLRFYASRLPVVEINN
                     TFYRLPKESVLQNWAEQVPPSFRFVLKAPQKITHIKRLKEASEEVEYLFRAENSLGPS
                     KGAVLFQLPPSLRKNLLTLQNFLSILPPDRPIAFEFRHPSWFDEEIFACLRERNCALC
                     LAEMEDSENIDLVPTANWGYLRLRRSDYTPTDLLTWKQRILAQPWDHAYVFFKHEDEG
                     IGPKLAAQFLDLGDVPTDLPG"
     gene            1854..2261
                     /locus_tag="VFY96_09355"
     CDS             1854..2261
                     /locus_tag="VFY96_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606709.1"
                     /translation="MFQITDGTFAEAKRYCIRDHQVLTEGSWYDPNSCWLNRFYSRNI
                     PSHAVEMTAAYLHKNVVAILGARLGKATMDEKERLAAVIHLCGVKHARSFAERGFRVT
                     TTERCGTHRVQTYFSQVNAMKDRFQRLRQSSVS"
     gene            2339..4366
                     /locus_tag="VFY96_09360"
     CDS             2339..4366
                     /locus_tag="VFY96_09360"
                     /inference="COORDINATES: protein motif:HMM:NF013395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEX5606710.1"
                     /translation="MKIPSRTVVAALDRAAKLPDGIPPPARFDYAKDRSRDTRPTAVA
                     RRAAFLSLGGARPTETELERLMGTDDLVDEFYLERALIAAQPVCRISIRSASGHERGC
                     ATGFMISPRLLLTNEHVFGSADEAEPSIAEFNYRLDIAGRAELSYQFRLRPDLFFFNH
                     ELLDFALVAVATSSVDHGVPLSRFGYHRLIAESGKGLIKEWMTIIQHPAGARRQFAIR
                     ENQCVEDRDPDVIWYMSDTAQGSSGAPVLNDSFQVVALHHSGVARQDDQNNYILKDGR
                     KVTSLTDVDDAEVDWIANAGIRVSRICSCIGAEAAEKNGFLAELKTAMEGGDVLSNAY
                     RQPARNNGFSIAAAFGGAEPGRFQIGSLVLELNTAHGAGQTSLPLFSGAPVALAPSSD
                     ETEALKEPIIDKDYSSRKGFDPQFLGRATPLPTVVNETAMAPMKNGSKIIPYEHFSVV
                     LHRERKLAILTAANVDGSAKARRPEAGKDYTRDGLSGLSKNHQEKWVLDSRVEPKFQV
                     PDGFYNRDQGAFDKGHIVRREDVCFGENYAQVRRANGDTFHVTNCSPQRASFNRAPGI
                     WGDLENFIGAQSDTERLCVFAAPILAASDKVFPGTEDVKLPSRFWKVVCAVKREKLQV
                     FAFVLEQDVHDLPLEFQVDAEWKHKQVSLKNLESMITLVKFPKLYHDADQA"
BASE COUNT         1052 a         1214 c         1234 g         1039 t
ORIGIN      
        1 agcggcgctg gcagcagccg atgatcatct cgatcgccgc caaagttccc ggcgccggct
       61 cggcattgtc ggcctcttct ctcgagatac tgccttcttc gagaaactgc agcatgatgt
      121 ccatatgcgt ttgtttgccg ccaagctctt ccatgaaatt ttccagcacg gtatctatca
      181 cactgtaatc ttttgcactc acagcgttga cggccatata tccgaaaaag atggggtttg
      241 tgcgaaccct caagtaatgc tggacttcgc cgagaatgat ttgctcgcgc gtccaacggt
      301 gcttaataat gccatcgaac cacggatgct gctccatggg aacttcggcg gcgtaacccc
      361 acagctcttc gataaattga tcggcatgca tgagctagga tctagctcag cgccaagaga
      421 atgtccacgg ggttctatct gctctcactg gacccaaaag ctgaaccttg ttacgacaag
      481 cgctataaca gtgaattccg cgatagcgat gactacgcgt ttgaaatcgg gcggtgcgcg
      541 gttgcgaggt gatgcgtgaa cttatgggtc ggaaccagcg gttatagcta taaggcatgg
      601 ctgggaagct tttatccgga gcgtcttccg gcaaaagaaa tgctccgatt ctatgcttca
      661 cggctccctg tggtcgagat caacaataca ttttaccgtt tgcccaaaga aagcgttttg
      721 caaaattggg ccgagcaggt tccgcctagt tttcgcttcg tcctcaaggc gccgcaaaaa
      781 attacgcata ttaagcgcct caaagaggcg agcgaggaag tcgaatatct cttccgcgca
      841 gaaaactcgc tgggtccaag taagggtgcc gtgcttttcc aactgccgcc atcgctgcga
      901 aaaaatttgc tgacactgca aaattttctc tcgattctgc cgccggaccg gccgatcgcc
      961 ttcgaatttc gccatccctc ctggttcgat gaagagatct tcgcctgtct gcgcgagcgc
     1021 aactgcgcgc tgtgtctggc tgagatggag gacagtgaaa acatcgatct ggtacccacg
     1081 gcaaattggg gttacctgag acttaggcgc tccgactata ctccgacgga cctgctcact
     1141 tggaaacagc ggattctcgc acagccgtgg gatcacgcct acgttttctt caagcatgaa
     1201 gatgagggca tcggtccaaa gctcgcggca caatttctcg acctcggtga cgtgcctact
     1261 gatttacctg gataggttac aagttcgttg cagaaggatc gtctcacaga gtgacagacc
     1321 ctgaagcgat tgtcatttcg agcaaagcga gaaatctttc ccatctaact ttgtccttca
     1381 ataagataat cgtgctaaaa tgcagttcca tccagaataa caagaccgtt cgtgcctgat
     1441 cgaatcgaga ctcacaaggt atctatgagc gcaatcgttt ttaccgctac gcgccgatat
     1501 tcgaaaccga ggtccaaagc gattccgcgc tatcgccgaa ggattcttag tggctggtac
     1561 tgtatcctcg cgatcggttg tttgttcttg ttcaactgga tttaccaaac cagccgcaaa
     1621 cctgcggaac tgctaagccc gatcagctcc gcttttgcaa aaagtccgca ggcgacatgg
     1681 cagagttatg cgccgctttt cgacaaatat tcgacagctg ttatctctgc tgagctgctc
     1741 gccgccctgg cgcaagtgga agccaatggg aatcccgtcg cttcccttac tggcgctgga
     1801 aatggtcgtg gtatccttgg gaaatttatc gcccggcatc gactgcgctc ggcatgtttc
     1861 aaattacgga cggaaccttc gccgaggcga aaagatattg catccgcgat catcaggttc
     1921 tgaccgaggg ctcatggtac gatccgaatt cttgctggct caatcgtttc tatagccgaa
     1981 atatacccag ccacgcggtc gagatgactg ccgcatatct tcacaagaac gtcgtcgcga
     2041 ttttgggtgc tcgtttgggg aaggcgacga tggacgaaaa agaaagatta gcggcggtta
     2101 ttcatctttg cggcgtgaaa cacgcaagaa gctttgccga gagaggattt cgcgtgacga
     2161 caacggagcg ctgtggaacg caccgtgtgc aaacgtattt ctctcaggta aacgcgatga
     2221 aggatcgctt ccaaagacta cgacaaagca gcgtttcgta acagccgctt atcgaattgg
     2281 gagaagcgcg tccgctcacc gatcgggtca gcagtagcat aggaggtaga accccccagt
     2341 gaagattccc agccgaaccg ttgttgcggc cttggatcgc gccgccaagc ttcccgatgg
     2401 gataccgcct cccgccaggt tcgattacgc gaaagatcgt tcccgcgata cgaggccgac
     2461 tgcagtggcg cggcgtgcgg catttctcag cctaggaggc gcgcgtccaa ccgaaaccga
     2521 gctcgaacgt ctcatgggga cggacgatct ggtcgacgag ttctatctcg aacgcgcact
     2581 aatcgctgcg caaccggttt gccgcattag tatccgctcc gcctcgggtc atgagcgcgg
     2641 ttgcgcgacc gggttcatga tctcgccacg cctgctgctc actaacgagc acgtcttcgg
     2701 ctcggctgat gaggcggaac catcgatcgc agaattcaat tatcggctgg acattgccgg
     2761 aagagccgag ctttcgtatc aatttcggct tcgccccgat ctgtttttct tcaaccatga
     2821 acttttggat tttgccctcg tcgccgtcgc aaccagttca gtcgatcacg gcgtgccgct
     2881 tagccggttt ggctatcatc gactgatcgc cgaaagcggc aaaggtctga tcaaggaatg
     2941 gatgacgatc attcagcatc cggccggcgc gcggcgacaa tttgccattc gcgaaaacca
     3001 gtgtgtggag gatcgggatc cggacgtgat ctggtacatg agcgataccg cgcagggatc
     3061 gagcggtgca ccggtgttga acgacagttt ccaagtggtc gctttgcacc attccggggt
     3121 agcccggcag gacgatcaga acaactatat cctgaaagat ggcagaaaag tgaccagcct
     3181 gactgatgtc gatgacgccg aggtggattg gatcgccaac gccggtattc gcgtgagccg
     3241 aatttgttcc tgtattggcg cggaagcggc cgagaaaaat ggttttctgg cggagctcaa
     3301 aaccgccatg gagggaggcg acgtcctgtc caatgcgtat cggcaacctg cgcgcaataa
     3361 cggcttctcc atcgccgcag cttttggcgg agctgagccc ggcagatttc agatcgggag
     3421 tctcgtttta gagctcaaca ccgctcacgg cgcgggacaa acatcgttac cgctattctc
     3481 cggggcaccg gtggcgcttg cacccagtag tgacgaaacc gaagcgctca aggagccgat
     3541 tatcgacaag gactattcat ctcggaaagg attcgatccc cagtttttgg gaagggcaac
     3601 gcctttaccg acggtcgtga atgagaccgc catggcgccg atgaaaaacg gcagtaaaat
     3661 tattccctat gagcatttca gcgttgtatt gcatagggaa cgaaagctgg cgatcttgac
     3721 cgcggccaat gttgacggta gcgcgaaggc gagacgtccg gaggctggca aagactatac
     3781 gcgcgatggt ttaagcggat tgagcaaaaa tcaccaggag aagtgggtgc tcgattcgcg
     3841 agtggagcca aagtttcaag tccctgacgg tttttacaac cgggaccagg gggcattcga
     3901 caagggacac attgtgcgac gcgaggatgt ctgtttcggc gagaactacg cccaagttcg
     3961 ccgagcgaac ggcgatactt ttcacgtgac caattgctcg ccgcaacgag cctctttcaa
     4021 tcgcgctcca ggcatctggg gcgatctgga aaattttatt ggcgcgcaat cagacaccga
     4081 gcgtctctgt gtttttgcgg caccgatttt agcggctagc gacaaagtgt ttcccggcac
     4141 tgaagacgtc aagctgccgt cgcggttctg gaaagtcgtc tgcgcggtca aaagggagaa
     4201 actccaggtg tttgcatttg tccttgagca agatgtgcac gatctaccac tcgaatttca
     4261 agtcgatgcc gaatggaaac acaaacaagt atcgctaaag aatctcgaat cgatgattac
     4321 cttggtcaaa tttcccaagc tttatcacga cgcggatcaa gcgtgagcgg acccgcgttg
     4381 acgaatttac gcccggggca ctagcggcgc aaagtttttt attctctagt gtcaacgtcg
     4441 gggatctttg caatctcgtc gagccaaacg gtcgcttcac tatcgctcgg tgcgcgataa
     4501 tcgccgcgcg gtgatagcga tcctcccgaa cccactttc
//
LOCUS       DASULZ010000458         3352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_969887, whole genome shotgun sequence.
ACCESSION   DASULZ010000458 DASULZ010000000
VERSION     DASULZ010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969887"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VFY96_09365"
     CDS             <1..605
                     /locus_tag="VFY96_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5606711.1"
                     /translation="LEVEDVEKVGARLREKYPSIQLIKRPINRPFAGISTHDPDGYVF
                     DLSQQAMTNRAEVYVEGEWKQDRYISHFVLRSLNPANLAKFYREVYELQELETPADDP
                     NHYLSDGRVTIIVAPWAITDYEGAGIEPRHMDHLGFRVEYVESFKKDLAALVNRNPAL
                     SPKPGGTEGHIRLKLLAQCKHGQFQLADPDGVPIDVAAAH"
     gene            complement(989..2032)
                     /locus_tag="VFY96_09370"
     CDS             complement(989..2032)
                     /locus_tag="VFY96_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5606712.1"
                     /translation="MTFLWPEMLWLMLIVPALVAGYFYILRRKQESAAKYASLTMVKQ
                     AIGTRQRFRRHIPPLLFLIALIAMIVAIARPAAIVMLPSQYQTIILAMDVSGSMRATD
                     VQPNRLAAAQAAAKAFVSEQPSNVRIGVVSFAATASVVQSPTQNREDIITAIDRFQLQ
                     RGTAIGSGIIVSLATIFPNAGIDVSSFIYGRRGARGVAIDQVGKSDQPPVKPVAPGSY
                     TSAAIILLSDGQRTTGPDSLEAAQLAADRGVRVFTVGFGTQSGETIGFEGWSMRVRLD
                     EETLKTIAHMTRGEYFYAGTAADLKKVYQVLNSKFLLEKKPMEISSLFAGAAALIAVT
                     SALLSLLWFHRIL"
     gene            complement(2013..2978)
                     /locus_tag="VFY96_09375"
     CDS             complement(2013..2978)
                     /locus_tag="VFY96_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5606713.1"
                     /translation="MNPPQGLSGTASADSILRRLEWTVIRRLDGLLQGDYQTLFRGFG
                     LDLADIREYQYHDDVRYIDWNVTARLQSPYVRVYNEDREITAWFLLDLSASIDFGAQV
                     NKRSVSAEFVTVLARLLTRHGNRIGALFYGDRVDTVIPARTGRRQALHILHRMLSRAD
                     ASRSSETDLGDLLRSGFRVIPRRSLVFIVSDFFSVPGWTKPLAHLAQRHEVIAVRLFD
                     PLEMELPDLGFVLMRDAETGEQLFVDTNDRRFRRRFAALAATHEQELRSAFGQAGVDA
                     LELSTNDDLVDAILRFADLRKRRRQLAAGATLTRPSRGSYDISMA"
     gene            complement(3072..>3352)
                     /locus_tag="VFY96_09380"
     CDS             complement(3072..>3352)
                     /locus_tag="VFY96_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX5606714.1"
                     /translation="ETAKFLSFGASPRASINVIEGAKALALLRGRSYVLPEDVQDLVP
                     DVFRHRLVLTYEALAEGITPDDLIQRIMQHIGAPEKPLDAHVKFAAGS"
BASE COUNT          788 a          982 c          935 g          647 t
ORIGIN      
        1 gcctggaagt cgaagacgtc gaaaaagtcg gcgcgcggct gcgcgagaaa tatccttcca
       61 tacagttgat caagcggccc atcaatcgcc cgtttgccgg aatcagcacc catgatcccg
      121 acggctacgt attcgaccta tcgcaacagg ccatgaccaa tcgggcggaa gtgtacgtcg
      181 aaggcgaatg gaagcaggat cgctatatca gccacttcgt gctgcgctcg ctcaacccgg
      241 cgaatctggc caagttctat cgcgaagtct acgagcttca ggagctcgaa acgcccgcgg
      301 acgatccgaa tcattatcta tccgacgggc gggtgacgat catcgtcgcg ccatgggcaa
      361 tcaccgatta cgagggcgcc gggatcgagc cgagacacat ggatcatctg ggatttagag
      421 tcgaatacgt ggagagcttc aaaaaagatc tcgctgcgct ggtcaatcga aatccagcat
      481 tatcaccaaa accgggaggc acggaaggtc acattcgcct gaaactgctg gcccagtgta
      541 agcacggtca attccaactc gccgatcccg acggcgtccc gatcgacgtg gccgcggcgc
      601 attaaaacgc cgtcacacat atattcgcgg tacaatgttc aaacaggcgg tcaaggcgcc
      661 acaccgaata aaatgagctg cagtgcgttc ccagtagagc actgataaat cccccaatgg
      721 ccccgtttgt caaaggggcg ggccgcagga ccgggggatt tgacccgaat ggtattttca
      781 taaagagtct ggcgctcgag atgacaaaat ggttgtcgtc gaagagatag acgcaattcc
      841 gggcaagctg gcgatagcta gcgcaaccct gaatccagaa tccgtaagcc gcatcccatg
      901 cgccgtccgg atgccggtcc cggatctcat ccggaataag ctttttaccg gcataagagg
      961 tttctcaatt agagttatgc caatcgcatc acaaaatgcg gtgaaaccag aggagcgaca
     1021 gcaacgccga cgtaacggcg atcagtgccg cggcgccagc aaatagcgag ctaatctcca
     1081 tcggcttttt ttcgagcagg aacttggaat tgagaacctg gtaaactttt ttcaggtcgg
     1141 ctgccgtgcc ggcatagaaa tactcgccgc gcgtcatgtg cgcgatcgtc ttgagcgtct
     1201 cctcatcgag ccggacccgc atcgaccatc cctcaaagcc gatcgtctca ccgctttgag
     1261 tgccgaaccc gaccgtaaaa acgcgcacgc cgcgatcggc ggccaattga gcggcttcga
     1321 gcgagtcggg tccggtcgtg cgctggccgt cgctcagcaa gataatcgcc gctgaggtgt
     1381 aacttcccgg agccaccggc ttcacaggcg gttgatcgga tttgcccacc tgatcgatcg
     1441 ccacaccgcg ggcccccctg cgtccataaa tgaacgagct cacatcgatg ccggcattgg
     1501 ggaagatggt cgccaacgaa acgatgatgc cgctaccgat cgccgtaccg cgctgcagct
     1561 ggaatcggtc gatggcagtg ataatgtcct cgcggttttg cgtcggtgat tgcaccaccg
     1621 acgccgttgc cgcgaacgag accacaccaa tccgaacatt cgaaggctgt tcggagacga
     1681 aagctttcgc cgccgcctgt gccgcagcga gacgattcgg ttgaacgtcg gtggcgcgca
     1741 tgctacccga tacatccatt gcgagaatga tcgtttgata ttgggagggt aacataacaa
     1801 tcgctgcagg acgggcgatc gccacgatca tcgcgatcag cgcgatcaaa aatagcagcg
     1861 gcggaatgtg tctgcgaaat ctctggcgcg tcccgatcgc ctgttttacc atcgtcaagc
     1921 tggcatattt tgctgcgctc tcttgcttac gacgcagaat ataaaaatat ccggccacta
     1981 aggccggaac gatgagcatg agccagagca tttcaggcca tagaaatgtc atagcttcct
     2041 ctcgagggcc gcgttaaagt cgcgcccgct gctaattgcc ggcgccgttt gcgaagatcg
     2101 gcgaagcgca aaatggcatc gactaagtca tcattcgtag acaattccaa cgcgtctacg
     2161 ccggcttgcc cgaaggcaga gcgcagctcc tgctcgtgag tggcggccag cgccgcgaac
     2221 cgcctcctaa aacgccgatc gttggtatca acaaacaatt gttcgcctgt ctcggcgtcc
     2281 cgcatcagga caaagccaag atcaggcagt tccatctcga gcggatcgaa cagacgcacg
     2341 gcaatcactt cgtgccgctg cgccagatgg gccaagggct tggtccagcc cggcacgctg
     2401 aaaaaatcgg agacgataaa caccaacgat cttcgcggta tcacgcggaa accgcttcgg
     2461 agcagatcac ccagatccgt ctcggacgag cgggaggcat ccgctcggga tagcatgcga
     2521 tgtaaaatat gaagcgcctg gcgtcggcct gtgcgcgcgg gaattaccgt gtctacccga
     2581 tcgccataaa aaagcgcgcc gatccggttc ccatggcgcg ttaacagacg cgccaacacg
     2641 gtgacgaatt ccgccgagac cgagcgtttg ttgacctgcg cgccgaagtc gatggaggca
     2701 cttaaatcca acagaaacca cgcggtaatt tcgcgatctt cgttatacac gcgcacgtag
     2761 ggtgactgaa gccgcgccgt cacgttccaa tcgatgtagc ggacgtcgtc gtgatactgg
     2821 tactcgcgta tgtccgcgag atcgagcccg aagccgcgaa acagcgtctg ataatccccc
     2881 tgcagcaggc catcgagacg gcggatgacc gtccattcga gtcggcgcag gatgctatcc
     2941 gcgctcgcgg taccactaag gccttgagga gggttcacca actgcctcca gaaacagaac
     3001 cggagtaatt gagcgatgga gtactggagt cgtgttccta tccatcactc cgttactcca
     3061 acactccaat cctacgatcc cgcagcgaat ttcacatggg cgtccagcgg cttttccggt
     3121 gcgccaatat gctgcatgat gcgctgaatc aaatcgtccg gcgttattcc ttcggcgagc
     3181 gcctcgtagg tcagcaccag gcgatggcga aatacgtcgg gcactaaatc ttgcacgtcc
     3241 tcggggagga catagcttcg accgcgcaga agcgcgagcg ccttggcgcc ctcgatgacg
     3301 ttgattgatg ctcgggggct cgcaccgaag gataaaaatt tcgcggtttc cc
//
LOCUS       DASULZ010000459         3732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_766617, whole genome shotgun sequence.
ACCESSION   DASULZ010000459 DASULZ010000000
VERSION     DASULZ010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766617"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VFY96_09385"
     CDS             <1..203
                     /locus_tag="VFY96_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606715.1"
                     /translation="NAIVVGKNLSCRPCGTHGGRRCPLGHAHCVEQVQAQTVLEAAEK
                     LLRNVERIHRRPEAFQPEFLTV"
     gene            200..1042
                     /locus_tag="VFY96_09390"
     CDS             200..1042
                     /locus_tag="VFY96_09390"
                     /inference="COORDINATES: protein motif:HMM:NF018047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HEX5606716.1"
                     /translation="MKTRVLQAKRVAQSGEFLAARGETDAKLIWGISPEGFARIVDRY
                     GNLLVVRQDQIEYIDVSLCEDDSLEGDRKPRYYGRGAVRVQGLANGHTAVIRRYFHGG
                     AFRRVTRDWFFTWPPRPFRELAITEELRRRGLRTLEVYAACVSRPFGPFYRGWFITRQ
                     LPEAEDLWSALKTGFAERLGFERVLRVVAESIAALHREGVYHADLNLKNILVRAEGAD
                     VAAYIIDFDKTKVFRGKLPKPLASKNLNRLERSVLKLDPDRKFFPATTWDRFVSYYDA
                     AFGF"
     gene            1020..2087
                     /gene="waaF"
                     /locus_tag="VFY96_09395"
     CDS             1020..2087
                     /gene="waaF"
                     /locus_tag="VFY96_09395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEX5606717.1"
                     /translation="MMQLSVFNFDPKRILLILHGSIGDVTRALPLATVLRNGFPKSYI
                     AWSVEPASRPLLEHNSAIDELIVFDRARGWRTLGPFLKQIREKHFDLVLDLQRILKSG
                     LISWATGAPRRLGFNRLDAKEFNWVFNNLHIEPFGESIPKIEHYFKFTDALGLPRQAP
                     QWDFFLSAAERASIKPHLSGIQRSFAVLFVGTRWESKRWFPTQIARCADLLHAEYHLD
                     VVLVGQKPDERLADEVVRKSKTAVTNLVGRTSLRQAIGIIELAKLAIGPDTGLMHIAA
                     AVRTPVISLWGPTRPQRTGPYGYGDLVIQGRAPCVPCNRRWCNIGRLCMQSITTAEIG
                     ERIAVALGCDDAKVVRDVEGV"
     gene            2071..2940
                     /locus_tag="VFY96_09400"
     CDS             2071..2940
                     /locus_tag="VFY96_09400"
                     /inference="COORDINATES: protein motif:HMM:NF018047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HEX5606718.1"
                     /translation="MLRASDYQLRCVGPWHLLVLPDAWSEQLQESVINHIRQLEPSKH
                     PQTIPLDVVGAAGEKHYFIKLFHCDGLGNALKNLFRVSKPKRFWRQGLSLSAAGFRVP
                     TVVAVGELRCFRLFRRGFVLTLRIPGQPLPLFLRNLEGASQVRHNLRLKRDGLARLAE
                     LVRQFHRLGFVHGDLIANNLLITADGGEPQFYFMDNDRTSCFPYWWSARGWKRNLVQL
                     NRMPLPGVTLQDRMRFFCRYLGRRRLSEPDHQLVRWLEQQTRKRRYECDGVGPQGNFR
                     RLMRFSDPITGID"
     gene            3061..3249
                     /locus_tag="VFY96_09405"
     CDS             3061..3249
                     /locus_tag="VFY96_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606719.1"
                     /translation="MKFATRFFCGLAGFVHFFALTRAEKDPVQRRYDWQILSYLLDPA
                     LLLSLLSDNIVAKPRKLI"
     gene            3250..>3732
                     /locus_tag="VFY96_09410"
     CDS             3250..>3732
                     /locus_tag="VFY96_09410"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5606720.1"
                     /translation="MKIALVHKRLDLKGGTERDLLETAVGLTGVGHEVHLFCSQYNVT
                     VPAAIRTHRVLAAPLGRTIRLWSFALNAQKEIRKNGCDLVINFGRLLDADLLRCGGGT
                     HRGFLQWMAIHGGMRRRSWQAISAYHQSLLAFEKRQFSSPQLKKIIAVSESVKSDIMA
                     N"
BASE COUNT          773 a          980 c         1078 g          901 t
ORIGIN      
        1 agaatgccat cgtcgtgggc aagaaccttt catgccgtcc ttgcggaacc cacggcggcc
       61 ggcgttgccc ccttggtcac gctcattgcg ttgagcaggt tcaagcacaa acagttctcg
      121 aagctgcgga gaaattgcta cgcaatgttg aacggatcca ccgccgtccc gaggccttcc
      181 agccggagtt tctcacggtt tgaaaactcg agttcttcag gcgaaaaggg ttgcgcaaag
      241 cggcgaattt ctagcagcgc ggggtgagac cgacgcgaag ctgatttggg gcatctctcc
      301 cgaaggattt gcccgcatcg tcgatcggta tggtaacctt ctggtggtgc ggcaggacca
      361 gatcgagtac atcgatgttt cgctctgcga ggatgattcg ctggaaggcg atcgcaagcc
      421 gcgctactac ggtcgcggcg ccgttcgtgt acagggtctc gccaatggac acacagcagt
      481 tatcaggcgc tattttcatg gcggcgcgtt ccgtcgcgta acccgggact ggttttttac
      541 ttggccgccg cgaccgtttc gtgagctagc cattaccgaa gagctgcgcc ggcgcggctt
      601 acgaacgctt gaggtttatg ctgcctgcgt gagccggcct tttggaccat tttatcgcgg
      661 ttggttcatt actcggcaat tgcctgaagc agaagacctc tggtcggctc tgaaaaccgg
      721 ctttgcagag cgtctgggct tcgaacgtgt cttgcgagtt gttgccgaga gcattgcggc
      781 gctccaccgc gagggcgttt accacgcgga tctgaatctg aagaacattc tggttcgcgc
      841 tgaaggagcg gatgtggcgg cctacattat cgacttcgat aaaacaaaag tgttccgggg
      901 taaattgcct aagccacttg ccagcaaaaa cctgaatcgt ctagagcgct ccgtgctcaa
      961 gctcgatccc gaccgaaagt ttttcccggc cacgacatgg gaccgattcg taagctatta
     1021 tgatgcagct ttcggttttt aacttcgatc caaaaagaat tttactgatc ctgcatggct
     1081 cgattggaga tgtcactcgg gcgctacctt tggcgacagt gctccgaaat ggatttccta
     1141 aatcttacat cgcctggtca gtggagccag catcgcggcc tttgctggag cacaactcgg
     1201 cgatcgatga gctgatcgtg tttgaccgcg cgcgtggctg gagaactctg ggtccatttc
     1261 taaaacaaat ccgggaaaaa catttcgatc tggtgctcga tttgcagcgt atcctcaaga
     1321 gtggattgat cagttgggcg accggcgcgc cacgacggct cggctttaac cgtttggacg
     1381 cgaaagagtt caactgggtt ttcaataatt tgcacatcga accgttcggc gagagcattc
     1441 cgaagatcga gcattacttt aagtttaccg atgctctcgg tctgccgcgc caagctcctc
     1501 agtgggattt cttcctgagc gcggcggagc gagcatcgat caaacctcac ctttcaggta
     1561 tccaacgatc cttcgcggtc ctcttcgtcg gcacgcgctg ggaaagtaaa cgatggtttc
     1621 caacgcaaat cgcccgttgc gcggatttgt tacatgcgga gtatcatctc gacgtcgtat
     1681 tggtcgggca aaagccggac gagagactgg cggacgaggt cgtcaggaaa agcaagactg
     1741 cggtgacgaa tctcgttgga cgcacgagcc ttcgccaagc gatcggtatc attgagctgg
     1801 cgaagctcgc catcggtccg gacaccgggc taatgcatat tgcggccgcg gtgagaacgc
     1861 cggtaatttc tctttggggg ccaacgcggc cgcagcgaac cgggccatac ggctacggcg
     1921 atctggtgat tcaaggtcgc gcgccctgtg ttccgtgcaa ccgcaggtgg tgcaatatcg
     1981 gcaggctctg catgcaatcg atcaccacgg ctgaaatcgg cgaaagaatt gctgtcgctc
     2041 tcggctgtga cgatgcgaag gtcgtccgcg atgttgaggg cgtctgatta tcagctacgg
     2101 tgtgtcggtc cgtggcacct gttggtcctg ccagacgcgt ggagcgaaca actccaggaa
     2161 tctgtcatca atcacatccg gcaactagag ccttcgaaac atccgcagac gatccccctc
     2221 gatgtcgttg gagcggctgg agagaagcac tatttcatca aactgtttca ttgtgacggg
     2281 ctgggcaatg cacttaaaaa tttattcaga gtatccaagc ccaagcgctt ctggcgccaa
     2341 gggctttcgc tttcagcagc aggcttccgt gttccgaccg tcgttgcggt gggtgaattg
     2401 cgttgtttta gactcttccg aaggggattt gttctgacct tgaggattcc cggtcaaccc
     2461 ctgccgcttt tcttgcgcaa tctagaggga gcttctcaag tgcgccataa tctaagactc
     2521 aaacgcgatg ggctggcccg tttagcggaa ttggtgcggc agtttcatag gctcggcttt
     2581 gtccatggcg acctgattgc taacaatctg ctgatcacgg cagacggggg agagccgcag
     2641 ttttacttca tggacaatga ccgcacgagc tgttttccat actggtggtc cgcgcgtgga
     2701 tggaaacgca atctggtgca actgaatcgg atgccgctcc ccggcgtgac gttgcaggat
     2761 cgcatgcggt ttttctgccg atacttgggt cggcggaggc tttccgaacc ggaccatcaa
     2821 ctcgtacgtt ggctcgaaca acagacgcgc aagcgccgct acgaatgcga cggagtcggc
     2881 ccccagggaa attttcgtcg gctcatgcgg ttttccgacc ccattaccgg tattgattga
     2941 taaggctccg gtattgacta cgatcggctc taaaggtggc cattcggcac ttttgcattc
     3001 tgtcggcgct gtgtggtcga aagagttgcg ccggactaaa gttcgagagc tgagtctcgc
     3061 gtgaaatttg caacgcggtt cttttgtggc cttgcggggt ttgttcactt ttttgcgctg
     3121 acgcgcgccg aaaaggatcc cgtgcagcgg cgctacgatt ggcaaatcct ctcatatttg
     3181 cttgatccgg ccttgctcct gtcgctcctg tccgacaata ttgttgccaa gccgcgtaag
     3241 ctgatttgaa tgaaaatcgc gctggttcat aaacgtctcg atttgaaggg aggcaccgaa
     3301 agggatcttt tagaaaccgc cgtcggtctt actggggtcg ggcatgaggt gcacttattt
     3361 tgcagccaat acaacgtgac ggttcccgcg gcgattcgca cgcaccgggt cctagctgcg
     3421 ccgcttggcc gaacgattcg gctttggagc ttcgcgttaa acgcccagaa ggagatacgg
     3481 aaaaatggtt gcgacctggt cattaatttt ggtcgattgc tcgacgcgga tttgttacgg
     3541 tgcggtggcg gcacacatcg tggctttttg cagtggatgg caatacatgg cggcatgagg
     3601 cgccgctctt ggcaggcaat cagcgcgtat caccaaagct tgctggcttt cgaaaaacgt
     3661 caattcagct ccccgcagtt gaaaaagatt atcgctgttt ccgagagcgt caaaagcgat
     3721 attatggcga at
//
LOCUS       DASULZ010000460         2069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_970254, whole genome shotgun sequence.
ACCESSION   DASULZ010000460 DASULZ010000000
VERSION     DASULZ010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970254"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..1414)
                     /locus_tag="VFY96_09415"
     CDS             complement(56..1414)
                     /locus_tag="VFY96_09415"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX5606721.1"
                     /translation="MAQDLRTFLDTVKGQNPDDFLVISREVDPVFEITAIAVKLEQEA
                     KRRPILLFEKVRGSDFPVLTNVHAGRARLAAAIHAKPEQMQRAYLRAMEKPLAPKIIP
                     KAPVKDVILTGDQVDLYKLPQVCHHQDDAGPYITAAISFAKDPNGESWNCAYNRLMIK
                     GRNRTSIHLTLAKHLWEFQRYAEKEGNPLPVAFAIGVHPAIALGCLAIGSIDEDERAI
                     MGGLLGEPLELVKCETSDVLVPAAAEMILEGEILPHERSAEGPFGEFTGYSLGERQRE
                     ILQVRAMTHRRDAMFQDITVGHLDHLMLSTTPIEANLYRAVRAMVPTVKAVRVPAPFT
                     CYVSIEQRISGQAKNAILAVLGADLYMKRVVIVDHDVDVFNDRQVNWAIATRCQPDRD
                     IAIISNTRGSDLDPSTKEDGNTAKWGVDATAKPSLAAFTPKHRIPADVWQRINIKEIL
                     GK"
     gene            complement(1422..>2069)
                     /locus_tag="VFY96_09420"
     CDS             complement(1422..>2069)
                     /locus_tag="VFY96_09420"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoyl-CoA reductase"
                     /protein_id="HEX5606722.1"
                     /translation="CNYYDQTFFWRQAVGFCMKKDGDICLVAPGSAKCLAVASSDIAP
                     VLVSLGAEAVLVGNHGERRVKLEQLYRDDGIEYSSKAPDEVLKALLIPRENLTRRNIY
                     LKLRRRGSFDFPILGVAATLFLDDSGECRDASVVLTAVASAPKVVNEAGVLLTGKKVT
                     LELIDAVAELAAKAAHPLDNTDLDYWYRKRMAKVYTQRALASLAGLNLPTATAAP"
BASE COUNT          433 a          584 c          587 g          465 t
ORIGIN      
        1 ggccggaatt gttaccatca ctccattact ccgatactcc atcactccag caaacctact
       61 tcccgagaat ttctttgatg ttgatacgtt gccatacgtc agccggaatg cggtgttttg
      121 gggtgaatgc agccagcgac ggtttggcgg tggcatcgac gccccatttt gcggtgttgc
      181 cgtcttcttt ggtggatgga tcgagatccg agccgcgggt attgctgatg atcgcgatat
      241 cccggtccgg ttgacagcgt gtcgcgatcg cccagttgac ctgccgatcg ttgaaaacgt
      301 cgacatcatg atcgacgatg acaacgcgct tcatgtagag atcagcgccg agcaccgcca
      361 ggattgcatt cttcgcttga cccgaaatcc gctgttcgat cgaaacgtag caggtgaaag
      421 gcgccggcac gcgcacggcc ttgacggtgg gcaccatggc gcgcacggcg cggtacagat
      481 tggcttcgat cggcgtggtc gaaagcatca ggtgatcgag atgaccgacg gtaatatctt
      541 ggaacatcgc gtcacgccga tgagtcatcg cgcgcacttg caagatttca cgttggcgct
      601 cgccaaggct gtagccggtg aactcgccga acggaccttc ggcggagcgc tcgtgcggta
      661 aaatttctcc ttcgagaatc atctccgccg cggcgggaac cagaacatcg gaggtctcgc
      721 atttcaccag ctccagcggt tcgccaagca aaccgcccat gatcgcgcgc tcgtcttcgt
      781 cgatggaacc aatcgcaaga cagcccagcg caatcgccgg gtggacaccg atggcgaagg
      841 cgaccggcaa cgggtttcct tctttttcag cgtagcgctg aaattcccaa aggtgttttg
      901 ccaaggtaag gtgaatcgag gtacggttcc ggcctttgat cattagccga ttgtaagcgc
      961 agttccagct ctcgccgttt ggatctttgg caaacgaaat ggcggccgta atgtaaggac
     1021 cggcgtcgtc ctgatggtgg caaacttgcg gcagcttata gagatcgact tgatcgccgg
     1081 tgagaatcac gtccttgacg ggcgccttcg gaataatctt cggcgcgagc ggtttttcca
     1141 tggcgcgtaa ataagcgcgc tgcatttgtt ccggtttcgc gtggatggct gcggctagcc
     1201 gcgcgcgccc ggcatgcacg ttggtcaaca cagggaaatc gctgcccctc actttttcga
     1261 aaagcagaat cggccgccgt ttggcttcct gttcgagctt caccgcgatg gcggtgattt
     1321 cgaagacagg atcgacttcg cgggagataa ccagaaaatc gtccggattc tgacctttga
     1381 cggtgtccag aaatgttcgc agatcttgcg ccataaggtt tttagggtgc ggctgttgca
     1441 gtcggcaagt tcaaacccgc aaggctcgcc aaagctcgct gcgtgtacac tttcgccatg
     1501 cgcttgcgat accaataatc caaatccgtg ttgtcgagcg ggtgcgcagc tttcgccgcg
     1561 agctcggcga cggcatcgat caattccaaa gtaactttct ttcctgtcag cagtactccg
     1621 gcctcgttga ccactttcgg cgccgaggcg acggcggtta acaccacgct ggcatcgcgg
     1681 cattcgccac tatcatcaag aaagagagtt gccgcgaccc cgagaatcgg aaaatcgaag
     1741 gagccgcggc gccgtagctt caggtaaata tttcgacgcg tcagattctc gcgcggaatg
     1801 agcaaggctt tcaggacttc gtctggagct ttgctcgaat actcgatgcc gtcatcgcga
     1861 tataactgtt cgagcttgac gcgccgttcg ccgtggttgc cgaccaaaac tgcttccgcc
     1921 cccaggctca cgagcaccgg cgcgatatcc gaagatgcca cagctaaaca cttcgcgctt
     1981 ccgggagcga ccaagcagat gtcgccgtct tttttcatgc aaaacccgac ggcctggcgc
     2041 cagaagaatg tttgatcgta ataattgca
//
LOCUS       DASULZ010000461         2177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1376832, whole genome shotgun sequence.
ACCESSION   DASULZ010000461 DASULZ010000000
VERSION     DASULZ010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376832"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VFY96_09425"
     CDS             complement(<1..545)
                     /locus_tag="VFY96_09425"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX5606723.1"
                     /translation="MHDAIDRAFDVIARVDRKMSFHHSDSDVSRINREAFQRRVTVDP
                     WTWRVLRAAQRLSHESDGIFDITVARKLMQWKYLPRHYRNVSKGNWRDIVLEKNRIVR
                     FRNRVVVDLGGIAKGFAVDRAVKALKCAGVRSGVVNAGGDLRVFGLRSELVHLRHPDE
                     PMRPAGAISLHERALASSGIY"
     gene            complement(616..1155)
                     /locus_tag="VFY96_09430"
     CDS             complement(616..1155)
                     /locus_tag="VFY96_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEX5606724.1"
                     /translation="MTHIPRWILPAAISATVPLPSYAAQYLTVSAAQKLCFPECDQFV
                     PAHVRFTPAQTKAIEANSGQKQLLRGQEIWRATQNGKLRGFFIVDYVIGKHLVIDYAV
                     ALSPEGSVKQIEILNYRESYGGEIRNPDWRKQFVGKRSGSRFALNNGINNISGATLSC
                     RHVTEGVRRVLATYEVCLK"
     gene            complement(1177..>2177)
                     /locus_tag="VFY96_09435"
     CDS             complement(1177..>2177)
                     /locus_tag="VFY96_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606725.1"
                     /translation="GGIAEDADDKLTGAIELEQAYVDFMFNEHFNWRSPGVDVVPFGF
                     INLFHEPTQFYSVNRPELDDGLIPTTWFEGSTSIFGKIVNNLNYQFQINTGLEDIGQN
                     GGVPDDGSPYEAGISGTEALDLARTPIGDFNQAKNAPGFALRLSYTPPFAPGLNGSSS
                     VFFTPNVTPRGAYGDEGNSLGQNSVTMLDTEVRYRIPRTGLELRGEVVDVIIGTPRNL
                     RANNDGDAENNVGKHLWGFSLEAAYHIDLSDYVHNGWELVPFYRYTYENLQTGGFGGT
                     DANLPTGQGQRQFHTLGMALFPTPQVVLKVDYQFALDNAPDSPRADHLLGAVGFFF"
BASE COUNT          503 a          598 c          581 g          495 t
ORIGIN      
        1 aaatatattc cagaggacgc cagggcacgc tcatgcaaac tgatggcgcc tgccggccgc
       61 atcggctcat ccggatggcg caaatgaacg agttccgatc tcaatccaaa cacgcgcagg
      121 tcgccaccgg catttacaac accggatcga actccagcac attttaaggc tttgaccgcg
      181 cgatcgaccg caaaaccctt ggcgattccg ccgagatcga caacaacgcg gtttcgaaat
      241 cggacgatgc ggttcttttc gagaacaatg tcacgccagt tccctttgct tacattccga
      301 tagtgtcggg gaagatactt ccactgcatc agtttcctcg cgaccgtgat gtcgaaaatg
      361 ccgtcacttt catgcgacaa tcgctgtgcc gctcgcaaga cccgccaggt ccagggatcg
      421 actgtgactc tgcgctgaaa tgcttcccga ttgattctcg aaacatcgct atccgaatgg
      481 tgaaaactca ttttccggtc caccctggcg atgacatcga acgcgcgatc gatagcgtcg
      541 tgcaatcttg cttcgttatc gcctcgagcg gtgatttcga caaacgtgcc gagagcggga
      601 cgggcccgcc gcactctatt tgaggcagac ctcataagtc gccaggacac gccgcacgcc
      661 ttctgtcaca tgccggcagg aaagcgttgc gccactgata ttgttgatgc cgttattgag
      721 agcgaaccgc gagccagatc gtttgccgac aaattgtttt cgccaatcag gattgcgaat
      781 ttcgccgcca taactttccc ggtaattcaa aatctcgatt tgtttaaccg atccctcggg
      841 cgaaagcgcc actgcataat cgatcacgag atgtttgccg atgacgtagt cgacaataaa
      901 aaacccgcga agttttccat tttgcgtggc gcgccagatt tcctggccgc gcaaaagttg
      961 tttttgtcca ctatttgcct cgatcgcttt ggtctgcgca ggtgtaaacc taacatgcgc
     1021 ggggacaaat tgatcgcact ccggaaagca caatttctgc gccgcggaga cggtcaggta
     1081 ttgcgctgcg taggaaggca gcggaacggt ggcgctgatc gcggcaggca ggatccagcg
     1141 tggaatgtga gtcatgccag ggcgattgag gctctcttag aaaaagaaac cgactgcccc
     1201 aagcagatga tctgcgcgtg gcgagtccgg cgcgttatcg agcgcgaatt ggtagtccac
     1261 tttcaagacg acttgtggcg tcgggaacag agccatgcca agagtgtgaa attgtcgctg
     1321 accctgaccg gttggcaaat tggcatcggt tccgccgaat ccgccagtct gcaaattctc
     1381 gtatgtatag cggtaaaacg gcacgagctc ccagccgtta tgcacgtagt cgctaagatc
     1441 gatatgataa gccgcttcga gcgagaaacc ccagagatgt ttacccacat tattttctgc
     1501 atcaccgtcg ttgttggcgc ggagattgcg cggtgtgccg atgatcacat caaccacttc
     1561 accacgtaac tcgaggccgg tccgcgggat acggtagcgc acctcggtat cgagcatcgt
     1621 tacactgttt tggcctaacg aatttccctc gtcgccatag gcaccgcggg gcgtgacgtt
     1681 cggagtaaag aaaacactcg agcttccatt taagccgggg gcaaacggcg gcgtgtaact
     1741 cagacgcagc gcaaagccag gcgcattctt ggcctgatta aaatcgccga tcggggtgcg
     1801 agcgagatca agcgcttcgg ttccgctaat tccggcttcg taaggcgaac cgtcatcagg
     1861 gacaccgccg ttttggccga tatcttccaa ccctgtgttg atttggaatt gatagttcag
     1921 attgttaacg atcttaccaa agatactcgt gctgccttca aaccatgtgg tggggatcag
     1981 tccatcgtcc aactccggcc gattcacgct atagaactgt gtcggttcgt gaaaaaggtt
     2041 gatgaagccg aaaggcacca catcaacacc gggcgagcgc cagttgaagt gctcgttaaa
     2101 catgaagtcg acgtaggctt gttcgagctc tatcgcgcca gtgagtttgt catctgcatc
     2161 ttcggcgatg ccgccgt
//
LOCUS       DASULZ010000462         2320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_57839, whole genome shotgun sequence.
ACCESSION   DASULZ010000462 DASULZ010000000
VERSION     DASULZ010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57839"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFY96_09440"
     CDS             complement(<1..294)
                     /locus_tag="VFY96_09440"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX5606726.1"
                     /translation="MISLEQANGIITAIFTRGRELRCRPLSVIIVEPGAKVKAFQKED
                     DSAMMRFEMAYGKAYAALALGRSSSLVRMRTEERPLFMQYLIRASGDQIFPEGG"
     gene            complement(500..1348)
                     /gene="trxA"
                     /locus_tag="VFY96_09445"
     CDS             complement(500..1348)
                     /gene="trxA"
                     /locus_tag="VFY96_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX5606727.1"
                     /translation="MNQWTIDVSEENFEAQVLERSNTVPVVVDFWAEWCGPCKALGPV
                     LEKLADEYGGDFLLAKVNVDENPGLASVFGVQGIPAVKLFKDGDLASEFTGALPEPMV
                     RQFLTKFLPSAADKQAQEAESLESNKADEARSLYQKILDSEPNHAKALLGLGRLALEA
                     DENQKAMEYLDRVPIIADERKEADRLIARLNLQSGAAESESALREKLKAEPNNIAARF
                     ELAQALAGVEKYEEALKEFLTVVKTDREFQDDGARKAMVQIFEVLGDHPLTDKYRSEL
                     AAALFR"
     gene            complement(1454..1936)
                     /locus_tag="VFY96_09450"
     CDS             complement(1454..1936)
                     /locus_tag="VFY96_09450"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255440914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(1751..1753),aa:Sec)
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX5606728.1"
                     /translation="MSEDLNVKREIIVEADAETLARMRKEGHARGFTVYCDEAERIGG
                     DNTAAPPLAYFALSVGFULLTQISRYGEMLKTHIDKAKVYVTARFKNEGSVLRETVQA
                     EGLGVETRCEIASGEAPEKLAKVIRNAEAGCYVIQTIRRPTEVKSSYVLNGKMFDPGS
                     "
     gene            2090..>2320
                     /locus_tag="VFY96_09455"
     CDS             2090..>2320
                     /locus_tag="VFY96_09455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5606729.1"
                     /translation="MPNKILIVDDEPFNLDLLEQELSDLGYSVVRAESGAAALTQIDK
                     MPPDLVLLDYLMPGMNGIEVLQTIRKTQNDLPI"
BASE COUNT          471 a          689 c          582 g          578 t
ORIGIN      
        1 gccgccctcc ggaaagatct gatcgcccga agcacgaatg agatattgca tgaagagcgg
       61 ccgttcttcc gtccgcatcc ggacgagaga cgacgagcgg cccagcgcca aagccgcgta
      121 ggctttgccg taagccattt cgaaacgcat catggccgaa tcgtcttcct tttgaaacgc
      181 tttcaccttt gcgccgggtt cgacgatgat cacgctgagc ggtctgcatc tgagctcgcg
      241 cccgcgggta aagatcgcag tgataattcc gttggcttgc tccaagctga tcatgcttca
      301 atcctcggtc cagtgtcgct cggcccaatt gtgtcattct gagccaccgg cgaaggatct
      361 tgctgttcac gctgtcgtta gattcttcgt cgcgctgctc ctcagaacga catcatcttg
      421 aaatgcactt tttctctcgt gcgctttgcg cctcggcggg gataagtctt tttctcatct
      481 ctggaaaaaa caattctgcc tagcgaaaaa gcgcggccgc aagctccgac cgatacttgt
      541 ccgtcaacgg atggtccccc agcacttcga agatctgcac catcgctttg cgcgcgccat
      601 cgtcctggaa ctcgcgatcg gttttcacca ccgtaagaaa ctccttcagc gcctcttcgt
      661 atttttcgac acccgcgagt gcttgcgcta actcgaacct cgccgcgata ttgttcggct
      721 ccgccttgag tttttcccgt agcgccgatt cgctctcagc ggcgccactc tgaaggttca
      781 accgggcgat taaacgatcg gcttctttcc gctcatcggc aatgatcgga acgcgatcga
      841 gatattccat cgccttttga ttttcgtcag cctccagtgc gagtcgtccg agtccaagca
      901 gcgccttggc atgattgggc tccgagtcga gaattttttg gtagagtgat ctggcttcgt
      961 cggctttgtt cgactctagg ctctcggcct cttgagcctg cttgtctgcc gcgctcggaa
     1021 ggaatttggt caaaaactgc cgcaccatcg gctccggtaa tgcgccggta aattcggatg
     1081 cgagatcgcc gtctttgaag agcttgaccg cggggattcc ctgcacgccg aagacgctcg
     1141 ccaatccggg attttcgtcc acgttcactt tcgcgagaag aaaatcgccg ccatattcgt
     1201 ccgcaagctt ttcgagcacc gggcccaacg ccttgcaggg gccgcaccac tccgcccaga
     1261 agtccacgac cactgggacg gtatttgagc gttccaaaac ttgagcttcg aaattttctt
     1321 cgcttacgtc gatcgtccat tgattcatag tctgctcaca gtgattaaac cgccgagggc
     1381 tgcgagttca cagaaataaa tctaacctgg tttaccaacc gtctctgtgt caacgtcatc
     1441 ccagtgactt tttctaactc cccggatcga acattttgcc gttgagcaca tagctgctct
     1501 tcacttccgt cggtctgcgg atcgtctgaa tcacgtagca acccgcttca gcgttgcgaa
     1561 taactttggc cagcttttcc ggtgcctcgc ctgaggcgat ctcgcagcgt gtttccacgc
     1621 cgagtccttc cgcctgaacg gtttcccgca gtaccgatcc ctcgttctta aaccgcgccg
     1681 tcacgtagac tttcgccttg tcgatatggg tcttgagcat ttccccgtac cgggaaatct
     1741 gtgtcagcag tcaaaacccc accgagagag caaaataggc aagcggcggc gccgcggtgt
     1801 tgtcgccgcc gatccgctcg gcctcgtcac agtagactgt gaagccgcgc gcgtgccctt
     1861 ccttgcgcat ccgcgccaac gtttcggcat cggcttctac gattatttcc cgtttgacgt
     1921 tcaaatcttc gctcataggg ccaatatatt cgtaattgtc gcccattgcc aagtacccga
     1981 atgaagctca acaagcgatc gagcgcaacg aagcaggggg cttcttcagg gacatgttta
     2041 gatttgcacg gtgctgcggc gcaaagttat agtgagcgcg aatcatcgta tgccgaataa
     2101 aatactcata gtcgacgacg aaccctttaa tttggatctt ctcgaacagg agctgagcga
     2161 tttaggctat tctgtcgtcc gcgctgaaag cggtgcggcg gccttgacgc aaatcgacaa
     2221 aatgccgcca gacttggtgc tgctcgatta cctcatgcct ggaatgaacg gcatcgaggt
     2281 tttgcagacg attcggaaaa ctcagaatga tcttccgatc
//
LOCUS       DASULZ010000463         3128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1477324, whole genome shotgun sequence.
ACCESSION   DASULZ010000463 DASULZ010000000
VERSION     DASULZ010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477324"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="VFY96_09460"
     CDS             <1..1119
                     /locus_tag="VFY96_09460"
                     /inference="COORDINATES: protein motif:HMM:NF018032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HEX5606730.1"
                     /translation="ENFAKYSGKFICAAAGHNHEALLAAYGCGAVDLSKSEHKTVLNV
                     DMGGGTTKFSLVEDGVVTQTASINIGARLIAFDDDDTITRIEDAGRTMMKELGYKVEL
                     GQKITEKMKEDFGTYMAKILFEIIEHGPKSPMAKSLMVTPPFVNYRGFTQVQHIVFSG
                     GVSEHVYDRDPKAYGDIGPVLGKNMREYLKKLPDGVLREPIEGIRATVIGAGEYTIQA
                     SGNTSYVSNEKALPVHGLKVIQAPIRDGDSVSAALRQSLRKFDLSRFTSGLALSLTVS
                     GVPDYQSVRRIAEGVAEILKEADDPKCPLYLTLDLDIAKSLGSMLKEEFKVARDIIAV
                     DGIEVGDLDYIDIGECLGITEVIPVTVKSLMFPTTHDD"
     gene            1309..2445
                     /locus_tag="VFY96_09465"
     CDS             1309..2445
                     /locus_tag="VFY96_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5606731.1"
                     /translation="MSKAMQAERKMWSKVYTYDEWMEAQRIPIYRGYHIEDLRTLQLS
                     WWEARQCHSAFIQLLGQEGVTSARVSEIPAGKTLPPLKFALDEIVYVVEGRGLTTIWA
                     GEKQPKKTFEWQKHSMFLLPHNYTHQFSNMQGDKPARLLHYNYMPLTLSGVRDPNFIF
                     GNSYEGPDIQNEMDFYSEAKMISQQNLDESWGRRAYWYGNFFPDMRAWDKLDSNARRG
                     AGGRSVYIQFPDSEMTCHMSVFDARLYKKAHRHGPGRVIVIPVGEGYSIMWEEGKEKV
                     VVPWHEASCFVPPNKWFHQHFNAGGEPARYLALHPPMQFHGHAEKVEDRAKDQIEYVN
                     EDPWVREKFESELAKRGLTSLMPPETYTNPDFEWNWKPFLKSGK"
     gene            2628..>3128
                     /locus_tag="VFY96_09470"
     CDS             2628..>3128
                     /locus_tag="VFY96_09470"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5606732.1"
                     /translation="MIIENHAKEKIFWPNQQRVAVAITFDFQGGEPVRPGPNGKLNNE
                     DYAEGEYGPKTGIWRILRMLDEVGVKTTFLTCGGIAERYPDAMKVITERGHEVASHGY
                     HHEIARDLTREQELDCIRRTTDMLLKRTGKRPVGWRTCTQSDNTVELLMEEGYVWNSN
                     SFADDLP"
BASE COUNT          750 a          846 c          891 g          641 t
ORIGIN      
        1 gagaacttcg cgaaatattc cggcaagttc atctgcgcgg cggcgggtca caaccatgag
       61 gcgctgctgg ccgcctatgg gtgcggcgcg gtcgatcttt ccaagagcga gcacaagacc
      121 gtcctcaacg tcgatatggg cggcggcacc accaagtttt cgctcgtcga agatggcgtg
      181 gtgacgcaga ccgcgtcgat caacatcggc gcgcgcctga tcgcctttga cgacgatgac
      241 acgattaccc gtatcgaaga tgccggccgc acgatgatga aagagcttgg ctacaaggtc
      301 gagctcggtc aaaaaatcac ggaaaaaatg aaagaggact tcggcaccta catggcgaag
      361 attcttttcg aaatcatcga gcacgggccg aagtcgccaa tggcgaaatc cctgatggtc
      421 actccgccgt tcgtcaacta ccgcggcttc acccaggtgc agcacattgt cttctcgggc
      481 ggcgtttccg agcatgttta tgatcgcgat cccaaggcct atggcgatat cggaccggtg
      541 ctaggaaaga acatgcgcga gtatctaaaa aaattgcctg acggggtgct gcgggaaccc
      601 atcgaaggga ttcgcgccac tgtgatcggc gctggcgaat atacgatcca agcgagcggg
      661 aacaccagct acgtttccaa tgaaaaagca ttgccggtgc acggcctgaa agtgattcag
      721 gcgcccattc gcgacggcga ctcggtgagc gcagcgctgc ggcagtcgct gcgcaagttc
      781 gacttgtccc gtttcacttc cggtcttgca ttgtctttga cggtgagcgg tgtgcccgat
      841 taccagtcgg tccggcggat cgccgaaggg gtggcggaaa tcctcaagga agccgatgac
      901 ccgaaatgcc cactctattt aactttggat ctggatatcg ccaaatccct cggcagcatg
      961 ctcaaagaag aattcaaagt cgcccgggat atcatcgcgg tggacggtat cgaggtcggc
     1021 gacctggatt acatcgacat cggcgaatgt ctgggcatta cggaggttat tccggtaact
     1081 gtcaaatcgc tcatgttccc gaccacccat gacgattgat cggcgcttga ttcatatcag
     1141 cctccaggaa cttcagtctg gtccgagctc ccaagtagcg cgacggtatt aggaccgctt
     1201 tccccgagta gcgtgaagcg cgtatcgagg gactatctat tccgagaaaa tatttgtttg
     1261 agattggctg ggtgatatga gtgaccaatc gttgagagga gcacgttaat gtccaaagcg
     1321 atgcaagccg aacgcaaaat gtggagcaaa gtgtacacct acgacgagtg gatggaagcc
     1381 cagcgtattc cgatctatcg cggttaccac atcgaggacc tgcggacgct gcagttgagc
     1441 tggtgggagg cgcgacagtg tcattcggcg ttcatccagc tcctcggcca ggaaggcgtt
     1501 acttcggcgc gcgtcagcga gattcctgcc ggtaagactt tgccgccgct taaatttgcc
     1561 ctggacgaaa tcgtttatgt cgtcgaaggt cgtgggctta cgacaatttg ggccggagaa
     1621 aagcagccga agaagacttt cgaatggcaa aagcacagca tgtttcttct gccccacaac
     1681 tacacgcatc agttcagcaa catgcaagga gacaaaccgg cgcgcttgct gcattacaat
     1741 tatatgccgc tcacgctctc gggtgtgcgc gatcctaatt tcatctttgg caactcctac
     1801 gaggggccgg acatccagaa cgagatggat ttctattccg aagcgaagat gatctcgcag
     1861 caaaatctag acgaatcctg gggacggcgc gcttactggt acggcaattt tttcccggac
     1921 atgcgcgcct gggacaagct cgatagcaac gcccggcgcg gcgccggggg ccgcagcgtt
     1981 tatatccagt ttcccgactc ggaaatgacc tgccatatgt cggttttcga tgcccgtctg
     2041 tacaagaagg cacaccgcca cggtcccggc cgagtcatcg tcattcccgt cggcgaagga
     2101 tattcgatca tgtgggaaga gggcaaagaa aaagtcgtgg tgccctggca tgaggcgagc
     2161 tgtttcgtac caccgaacaa atggtttcat cagcacttca acgccggcgg cgagccggca
     2221 cgctatcttg cgctgcatcc gccgatgcag ttccatgggc acgcagagaa agtcgaggac
     2281 cgagccaaag atcagatcga gtacgtcaac gaggacccct gggtccggga aaagttcgaa
     2341 agcgagctgg ccaagcgcgg cctcacgtcg ttgatgccgc cggagaccta caccaatcct
     2401 gatttcgaat ggaattggaa gccgtttttg aaaagcggaa aatagtgagg cgaattcctc
     2461 aatcctcata ggcggtccct agatgtcacc tcgaaccaca ggtgagagat ctttcttaat
     2521 ccgaaccggt ttcatgtttg cgctggtgga ttgacacgac ggaaacttct cgtttagagt
     2581 gccaggactg cgtagtattc cgatcggtaa atcataggag gtccggaatg atcatcgaga
     2641 atcatgccaa agagaaaatc ttctggccca accagcagcg cgtcgcagtt gcgatcacat
     2701 ttgattttca gggcggcgag cccgtgcggc ccggtcccaa tggcaagtta aacaacgagg
     2761 attatgcgga aggggaatat ggtccgaaga ccggcatttg gcgaattcta agaatgctcg
     2821 acgaagtcgg tgtgaaaacc acgtttctca cctgcggcgg catcgccgaa cgatatcccg
     2881 atgccatgaa ggtgatcacc gaacgcggcc atgaagtcgc cagccacggc tatcaccacg
     2941 agatcgcacg cgatctcacg cgtgaacagg agctggactg catccggcgc accaccgaca
     3001 tgctcctgaa gcgaaccggc aagcggcccg tgggctggcg cacctgtact caaagtgaca
     3061 acaccgtcga attgctgatg gaagaaggct acgtctggaa cagcaactct ttcgcagacg
     3121 atctcccc
//
LOCUS       DASULZ010000464         5048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_767104, whole genome shotgun sequence.
ACCESSION   DASULZ010000464 DASULZ010000000
VERSION     DASULZ010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5048
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767104"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VFY96_09475"
     CDS             complement(<1..316)
                     /locus_tag="VFY96_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606733.1"
                     /translation="MAKDLPSFIAQQGAKREGSIIREKNFVDANNYETVAYLKHLDVR
                     NEPKMVLFENVPALNGQKSAFPMFYNPWVTRQYVADSLDLGDIKSPMDVSLEVARLEQ
                     QQG"
     gene            complement(389..1918)
                     /locus_tag="VFY96_09480"
     CDS             complement(389..1918)
                     /locus_tag="VFY96_09480"
                     /EC_number="1.2.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF006916.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinic semialdehyde dehydrogenase"
                     /protein_id="HEX5606734.1"
                     /translation="MNPQTLVVRNPATLEKIAELPVAHSNDVAIAVTQARKAQQTWAK
                     TSFAERAKLLYKLRDRLLDEGDRLADIMTGETGRPRAEVYGNELFYLCDAIGSWAKHS
                     ARYLSAKKITPSFPLLKTKRVISTYAPRGVIGIISPWNFPLTLTLGDALPALMAGNAV
                     VIKPSEVTPLSAMFGAEIIAQTGFPENLFQVVIGGAETGEALVDCVDMIAFTGSIEIG
                     KRVMKRAAERLIPVGLELGGKDPMIVLKDANLDRAVGAAVWGALVNCGQACTSIERVY
                     VEAPVYDRFVEKLVTRVRAIRQGGSENDVDIGCLTTEAQLKKVTAQVEEAIAQGAKAL
                     TGARRNPKLPGYYYEPTVLVDVDHSMSIATEETFGPVIPVIRVKDSQEALRLANDSHY
                     GLGGSVFSRDKQKAWQLAQDLQTGSVCINDSLINFIVPEAPMGGCKDSGFGYRHGAEG
                     MRKFCQQKTIVVDRFGFKEEFPWYPTSQRKAKQIRHLLNLLCHTGVRHKLRALRELAR
                     S"
     gene            complement(1979..2755)
                     /locus_tag="VFY96_09485"
     CDS             complement(1979..2755)
                     /locus_tag="VFY96_09485"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEX5606735.1"
                     /translation="MAMLGNKVALVTGGATGIGRAIAIELARNGARVAIASRSLSRIK
                     STADELRTAGFSALSVQMDVRKKRDVEAGIAATVSEWGTLHILVNNAGISGLSLMTDA
                     DDAKWFDIIDTNLNGLYLVTKAALRHMADGAGRVINIASVLGRFGVPGYTAYCATKHG
                     MIGFSRALALEVVDRGITVNTICPGWVETEMATLGISESAALQGITPDEFKARAIAAV
                     PIKRFLQAEEIAALVAYIASEAAAGITGQAINICGGQIMN"
     gene            2968..4032
                     /locus_tag="VFY96_09490"
     CDS             2968..4032
                     /locus_tag="VFY96_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEX5606736.1"
                     /translation="MDITKLLAFGVEQGASDCHLSAGEPPLIRIDGELKKLESPPLSK
                     EDVHTLVYDIMNDAQRKTFEQTHECDFSFEMGQVARFRVNVFMQRKGEGAVFRTIPTK
                     VLTLEELGMPPILKQLCDKEKGLILVTGPTGSGKSTTLAAMIDYLNSTFEGHILTVED
                     PIEFVHPSKKCLVNQRELGPHTHSFANALRAALREDPDIILVGEMRDLETIQLALTAA
                     ETGHIVFGTLHTSSAPKTVDRIIDVFPPNQQAQIRAQFAESIEAVLTQSLLKKKAGGR
                     VAALEILTGTTAVRNLIREGKIHQIPGTMQVSQKDGMQTMDMALTGLVTRGLVTREEA
                     QSKSMNPNLFGAAPVAAARV"
     gene            4047..>5048
                     /locus_tag="VFY96_09495"
     CDS             4047..>5048
                     /locus_tag="VFY96_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX5606737.1"
                     /translation="MESKSEIQKFFNTMVETEASDLYLTVAKPPMYRIDGKIRAVGDD
                     AFSPQNLMDLAETMMNEKQRKDFGETLEMNMAMSLPGVARFRVNIFRQRGSVGMVIRR
                     IKAEVMSLDQMGLPSILKDIVMTKRGLVLVVGATGSGKSTSLAAMIDHRNSSEAGHII
                     TIEDPIEFVHRHKESIVTQREVGFDTHSFQSALKNTLRQAPDVILIGEIRDTETMEAA
                     IAFAETGHLCLGTLHSNNANQAIERIMNFFPTERHAQIYLQLSLNLRAIVSQRLIPSV
                     DGKRMAALEILMDIPRVKDLIKKGETDTLKEAMEQGVQEGCQTFDQALFNLYKAEKIT
                     "
BASE COUNT         1162 a         1361 c         1351 g         1174 t
ORIGIN      
        1 ttccttgttg ctgctccagc cgcgcgactt cgagactgac gtccataggc gatttgatat
       61 ctcccagatc gagagagtcg gcgacgtact gccgagtgac ccacgggttg taaaacatcg
      121 ggaaggccga tttctggcca ttcagcgccg gaacgttttc aaatagaacc atcttaggtt
      181 cgttgcggac gtccaggtgt ttcaaataag cgacggtctc ataattgttc gcatcgacga
      241 agtttttctc gcgaatgatc gaaccttctc ttttggcgcc ctgttgagcg ataaagctcg
      301 gtaggtcttt ggccatgaaa ttctcctcta atctcgacga ataatatctc acctaattag
      361 atgaatcagg tgacgagttc aagggtactt aactgcgtgc cagttctcgt agcgcacgca
      421 gcttgtggcg gacgcccgtg tgacacaaca ggttgagcaa atgacgaatc tgcttggctt
      481 tcctttgtga ggtgggatac caggggaact cttccttgaa cccgaatcga tcgacgacga
      541 tggtcttttg ctgacaaaat tttcgcatgc cctccgctcc atggcgatag ccaaaaccgc
      601 tgtccttgca tccgcccatc ggggcctcgg gcacgataaa attgatcaag ctatcattga
      661 tacaaaccga gcccgtctgt aaatcttgcg cgagctgcca ggccttttgt ttgtctcggg
      721 aaaacacgct gccgccgagc ccataatggg aatcattggc gagccggagc gcctcctggg
      781 agtccttgac tctgatgacc gggatcacag gcccaaaggt ctcttcggtg gcaatcgaca
      841 tgctatggtc gacatcgacg agcaccgtcg gctcataata atatcctggc aatttgggat
      901 tgcgccgggc tccggtcagt gctttggcgc cttgcgcgat cgcttcttcg acttgcgccg
      961 tgactttctt gagttgggct tcggttgtga ggcagccaat atcaacgtca ttctcgctcc
     1021 cgccttggcg gatggcgcgt acccgcgtca ccagcttttc gacgaatcga tcataaactg
     1081 gcgcttcgac gtaaacacgc tcgatcgaag tgcaggcctg cccgcaattc accagcgctc
     1141 cccagacggc tgcgccgacg gcgcgatcga gattggcgtc cttaagcaca atcatcggat
     1201 ccttgccacc gagctccagg ccgactggaa tcagacgctc cgcggctctt ttcatcacgc
     1261 gtttgccgat ttcgatgctg cccgtgaagg cgatcatatc gacacaatcc accagggctt
     1321 ctccagtttc tgcaccgccg atgacgactt gaaaaagatt ttctgggaag cccgtttgag
     1381 cgataatctc ggcgccgaac attgccgata acggcgtcac ttctgatggc ttaatgacga
     1441 cggcattccc cgccattaga gctgggagcg catcccccag cgttagcgtc aatggaaaat
     1501 tccacggact aatgatgccg atcactcctc gaggcgcata ggtggaaata accctctttg
     1561 ttttaagaag ggggaaggac ggtgtgattt ttttcgcact gagatagcga gcgctgtgtt
     1621 tagcccacga gccgattgca tcacaaagat aaaacagctc gttgccgtac acttcggcgc
     1681 gcggtctgcc cgtctcgccg gtcatgatat cggcgagccg atcgccttca tcgagcaagc
     1741 gatcgcgcag tttgtaaagt aattttgcgc gctcggcgaa gctggttttg gcccacgtct
     1801 gctgcgcttt acgggcctgc gtgacggcaa tcgcaacatc attgctgtgc gccaccggca
     1861 actcagcgat tttttcgagc gtcgcgggat ttctgacgac aagagtctgt ggattcataa
     1921 ttaggtcaag caggcaaatg gcgcgggcgc gtgaagtcgg acggtgcccg cgcggtactc
     1981 agttcatgat ttggccgcca cagatgttga tggcttgtcc ggtaattcct gcggcggctt
     2041 ccgatgcgat ataagcaacc aatgccgcga tttcctcagc ttgcaaaaat cttttaatgg
     2101 gcaccgcggc gatggctcgc gccttgaatt cgtcgggtgt gatgccttgc agcgcggcgc
     2161 tttcactgat tcccaacgtc gccatttctg tttcgaccca gccaggacaa atagtattga
     2221 cggtgatgcc gcgatcaaca acctccaacg caagcgctcg gctgaagccg atcatgccat
     2281 gcttggtcgc gcagtaagcc gtatatccgg gcacgccgaa ccttcccagc accgaagcga
     2341 tattgatcac ccggccagct ccgtcagcca tgtgtcgtag cgcggctttg gttacgaggt
     2401 acaagccatt gaggttagta tcaatgatgt cgaaccactt ggcatcgtca gcatccgtca
     2461 tcaagctgag gcctgagatg ccagcgttgt ttacaagaat gtggagcgtc ccccactcag
     2521 aaacagtcgc ggcaatcccc gcctcgacat ctcgtttttt tcgaacgtcc atttgaacac
     2581 tgagagcgga aaagccggcg gttcgaagct cgtcagcagt gctcttaatt cggctgaggg
     2641 atcgcgacgc gatagctact ctggcgccat ttctagccag ttcaatggct atcgctctgc
     2701 cgattccggt ggcgccgccg gtcaccagcg cgactttatt gcccagcata gccatcaaat
     2761 ttcctcaaat tatcagcaca agtaccggct tttgcctgtg aaagcaactt tttagcttgc
     2821 accggcagaa ttttttcggg ggtcatcagg tatgtgctgt ggcgcgctat tcttacgtct
     2881 gaaagttctg ccttcgcgaa aaaattttta cagttttgga gccggataga ttacaatttt
     2941 agctctctaa taggagcaga catctttatg gatatcacaa aacttttggc cttcggcgtg
     3001 gaacagggtg cctcggactg tcatttgagc gcgggagaac cgccgcttat tcgtattgac
     3061 ggtgaactca agaaattgga aagcccgcct ctgagtaagg aggatgttca cacactggtt
     3121 tacgacatta tgaacgatgc tcaacgaaag acgttcgagc aaacacacga atgcgatttc
     3181 tcattcgaaa tgggtcaggt ggcgcgcttt cgagtgaatg tctttatgca gagaaaaggc
     3241 gaaggcgcgg tgtttcgtac cattccgacg aaagtgctga ccctcgagga gctcggtatg
     3301 ccgccgatcc tgaaacagct gtgcgacaaa gaaaaaggtt tgattctcgt cacgggtccc
     3361 accggatccg gaaaatctac aacactggcg gctatgatcg attatttgaa cagcacgttc
     3421 gaaggacaca ttctgaccgt cgaagacccg atcgagttcg tccacccatc caaaaaatgc
     3481 ctcgtcaacc agcgggagct cggcccgcac acgcactcgt ttgccaatgc gctgcgcgcc
     3541 gcgttgcgcg aagatccgga tatcattctg gtcggcgaaa tgcgcgattt ggagaccatc
     3601 cagctcgcgt tgacggcggc cgaaacgggg cacatcgttt tcggtacatt gcatacctcc
     3661 agcgcgccca agaccgtcga ccgtatcatc gatgtttttc cccccaatca gcaagcgcag
     3721 atccgggcac aattcgccga atcgatcgaa gcggttctca cgcaatcttt gctcaagaag
     3781 aaggccggcg gacgagtagc ggcgctagaa attttgaccg gaaccactgc tgtccgcaat
     3841 cttattcgcg agggcaagat tcaccagatt cccggcacca tgcaggtgag ccagaaggac
     3901 ggcatgcaga ccatggacat ggcgctgacg ggtctggtga cgcgcggcct ggtaactcgc
     3961 gaggaagcac aatcgaaaag catgaatcct aatctctttg gcgcggcgcc ggtcgccgcc
     4021 gcacgcgttt gacgagagtg gagttaatgg aatcgaagtc tgaaattcaa aagttcttca
     4081 atacgatggt cgagacggag gcatcagacc tctatcttac ggtggcaaag ccgcccatgt
     4141 atcgaatcga cggtaaaatc cgcgctgttg gcgatgatgc cttttctccg caaaatctta
     4201 tggatcttgc cgagacgatg atgaacgaaa agcagcgcaa ggacttcggc gagaccttgg
     4261 aaatgaacat ggcgatgtct ttacccggag tggcgcgctt tcgcgttaac atttttcgcc
     4321 agcgcggatc ggtcggcatg gtcattcgtc gcatcaaagc cgaggtcatg tccctcgatc
     4381 aaatggggtt gccatcgatt ctcaaagata ttgtcatgac aaaacgcggg ttggttctgg
     4441 tggtcggagc tacaggttcg ggcaaatcca catcgctggc agcgatgatc gaccatcgaa
     4501 actcgagcga ggcgggccac atcatcacca tcgaagaccc gatcgagttc gtgcaccggc
     4561 ataaagaatc gattgtgact cagcgcgaag ttggatttga tactcattcc tttcagtcgg
     4621 cgttgaaaaa taccctacgc caagcgccgg atgtgatctt gatcggtgaa attcgcgaca
     4681 ccgaaaccat ggaagcggcg atagcattcg ccgaaaccgg tcacctctgc ctcggcacgc
     4741 tgcactcgaa caatgccaat caggctatcg aacggattat gaacttcttt ccaaccgagc
     4801 gacacgcgca gatctatttg caactgtcct tgaatttgcg agcgatcgtg tcgcaacggt
     4861 tgattccgag cgtcgatggt aaacggatgg cggcgctcga gatcctgatg gatatccctc
     4921 gtgtgaagga tctgatcaag aaaggcgaga ccgatacttt gaaagaggcg atggagcaag
     4981 gcgtgcagga aggctgccag acattcgatc aggcgctctt taatctatac aaagcggaaa
     5041 aaattact
//
LOCUS       DASULZ010000465         2974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_58050, whole genome shotgun sequence.
ACCESSION   DASULZ010000465 DASULZ010000000
VERSION     DASULZ010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58050"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(281..1321)
                     /locus_tag="VFY96_09500"
     CDS             complement(281..1321)
                     /locus_tag="VFY96_09500"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5606738.1"
                     /translation="MRPCWGFATLCLVLIIACSGVGNEPIPGQPAHHVVGGFRNVNPD
                     FRRPSAWTRWSFVVRRFLSTSLAPRNFDAPRTANDGAILKAGDLNPSITWVGHATVLV
                     QINGVNIVTDPQWSERASPVSWGGPRRLSSPGLAFEDLPRIHVVLISHDHYDHLDLET
                     VKRLAAAHDPLFLVPLGLKAWFAENGMTRVEEMDWWQEREYRGLKFVCVPAQHFSQRT
                     LTDANQRLWASWSVLNAQRRFYFSGDTGYFTFFKEIGLRLGPFDVAALAIGAYLPPEM
                     MKFVHLKPEEAVQAFMDLNARALLGIHWGTFDLADEPPEEPPQRLVAEIRRRNIDPSR
                     AWIFKIGETRPW"
     gene            complement(1469..2452)
                     /locus_tag="VFY96_09505"
     CDS             complement(1469..2452)
                     /locus_tag="VFY96_09505"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606739.1"
                     /translation="MRKLLIVVLVCAAAALTAKNLGAQSLRAGYVSKDFNYLPFFIAQ
                     KNGFYAKEGIHVDLVNIGRADIQLQALVAGELHFANINADNIIIWNERTNGNLKVAAG
                     SSNSAPYLLIGAKNIKKIEDLKGQRLGVASLTGGATSILLAYLKSKGLVHPRDFSLSI
                     IAGGTPARLSALDSNAIAGAVLGIPFADVAIDKGFNRLGDTTEVISSYQFNNININPS
                     WAEKNRTAVVNFIKGHIQALRWSFAHPGEAAEFVTKEFGLQSKYAVRGIEYYTKNKVY
                     PINGDVTLSGLKVNIEVQAQDGIIKGSLPPPEKYIDLSYLRQAQKELGLEK"
     gene            complement(2519..>2974)
                     /locus_tag="VFY96_09510"
     CDS             complement(2519..>2974)
                     /locus_tag="VFY96_09510"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5606740.1"
                     /translation="SGYLTDRFGPRIPATIGVLLMIVSLASGGLLRADSHWFLPAALI
                     IVGAMTSGIFNPANSTAMISMMPREHRGFASAMNHVTFGFGNVFGVALGGLCMSLALE
                     FHTGMKVATLTAENPAGFVAALNTTFVAAIVLCVVGVFTSALRGTAKPA"
BASE COUNT          656 a          856 c          819 g          643 t
ORIGIN      
        1 tgatgtgcag ctgcgggctt ttgtcgagat ccttctcccg gtccatccac tcgatctcaa
       61 gcccttcttt ctcgaacaag ccctcgtcat aggccacgag ctcggccaat ccttgaaacg
      121 gcgcagtggt ttccaatcgc agtgttttca tattcctctc catggggcgt tgaaatttat
      181 ccctatacaa tggctggcat tccaacgtct gtcaaacgct gtggggacgc agttgtggag
      241 atccgatgcc gaactcctac gggcggtcct ataagttaac ttaccagggc cgggtttcgc
      301 cgatcttgaa aatccaggcg cgcgatggat cgatgttgcg acgacggatc tcagcgacaa
      361 gacgctgggg cggttcctcg ggcggttcgt ccgcgagatc gaaggtgccc cagtggatgc
      421 cgagcagcgc gcgggcattg agatccatga acgcctgcac cgcttcttcc ggcttgagat
      481 gcacaaattt catcatttcg ggtggtagat aagccccgat cgcgagcgca gcgacgtcaa
      541 acggaccgag ccgcaggccg atttccttga agaacgtgaa atatcccgta tcgccggaga
      601 aataaaaccg ccgctgggca ttcaacaccg accaggaggc ccacagtcgt tggttggcgt
      661 ccgtcagcgt tcgttgggag aaatgctggg ccggcacgca gacaaacttt agcccgcggt
      721 attcgcgctc ctgccaccaa tccatttctt cgactcgcgt cattccgttc tcggcaaacc
      781 atgccttgag accgagcgga accaaaaata gcggatcgtg cgccgcagcg aggcgcttta
      841 ccgtctccag gtcgagatga tcgtagtgat cgtgggaaat caaaaccaca tggatgcgcg
      901 gcagatcttc gaatgcgagt ccgggagagc tcaggcggcg cggcccgccc cacgagaccg
      961 gactcgctcg ttcactccac tgcggatcgg tgacaatatt gacgccattg atctgcacca
     1021 gcacggtggc gtgcccaacc caggtaatgc tcggattcaa atcgccggcc ttcaggattg
     1081 ccccgtcgtt cgcggttcgc ggcgcatcga aattcctcgg cgctaacgaa gttgataaga
     1141 atcgccgcac aacgaagctc cagcgggtcc atgcggaggg gcggcgaaaa tccggattga
     1201 cattgcgaaa cccgcccacc acatgatgag cgggttggcc ggggatcggc tcgtttccca
     1261 cacccgaaca ggcgataatt aaaaccaaac aaagtgtggc aaacccccag cacgggcgca
     1321 tactttcccg ttattgaacc attttcattt cgacgaaagc tagtccgagc tgatgtgatc
     1381 tcaaatggtt cgaactggca gagtgacctt cgatcagatg gcacagtgtg gtgatgaact
     1441 tgggcgaccg ggaatggctc gccacgactc acttctccaa cccgagctcc ttctgcgcct
     1501 gacgcagata gcttaaatcg atgtattttt ccggcggcgg cagtgaccct ttgataatac
     1561 cgtcttgcgc ctgcacttcg atattgacct tcaagccgct gagcgtcacg tcaccattaa
     1621 tggggtaaac tttgtttttc gtgtagtact cgatgccgcg caccgcatat ttcgattgca
     1681 agccgaactc tttcgtgaca aattccgccg cctcacccgg gtgagcgaaa ctccagcgca
     1741 acgcttggat atgacccttg atgaaattca ccaccgccgt gcgattcttt tcggcccagg
     1801 atggattgat attgatgtta ttgaactgat agctggaaat aacttcggtg gtgtcgccga
     1861 gacggttgaa tcctttgtcg atggcaacat cggcgaacgg aatgcccagc accgctcccg
     1921 cgatagcgtt gctgtccagg gcgctcaacc gcgccggcgt accgccagca atgatcgaga
     1981 gcgaaaaatc ccgcgggtgc accagtcctt tgctcttgag atacgccagc aagatcgatg
     2041 tggcaccgcc ggtgagactc gccacgccaa gcctctgacc cttgagatct tctatttttt
     2101 ttatgttctt ggcgccgatc agaagataag gcgccgagtt gctcgacccc gccgcgactt
     2161 tcaaatttcc gttggtccgt tcgttccaaa tgatgatgtt atccgcgttg atgttggcga
     2221 agtgcaattc cccggcgacc agcgcctgca gctggatatc ggcccgcccg atattgacga
     2281 ggtccacgtg gatgccttcc ttggcataga acccgttttt ctgcgcgatg aagaagggca
     2341 gatagttgaa atccttcgat acgtatcctg ctctgaggct ttgcgctccg agattcttcg
     2401 cggtaagcgc cgcggccgca caaaccagaa cgacgatgag caatttgcgc atgttgtgtc
     2461 ctcccttgca aagaaagctt gaacgatgcg cggacagaca agcagggtct tcagttcctc
     2521 aggccggctt ggctgtgccg cgaagcgccg atgtgaaaac tcccacgacg cacaatacaa
     2581 tagctgctac aaaagtggta ttgagggccg cgacaaagcc tgccggattt tccgccgtga
     2641 gcgtcgccac tttcatgccg gtatggaatt caagcgccag cgacatgcaa agcccgccga
     2701 gtgccacgcc gaaaacattg ccgaaaccga acgtcacatg attcatagcc gaggcgaacc
     2761 cgcgatgctc cctcggcatc atgctgatca ttgccgtgga attggctgga ttgaaaatgc
     2821 cgctggtcat ggcgccgacg atgatcaacg ccgcggggag gaaccaatgg gagtccgctc
     2881 gcagcagtcc gccactggcg agggatacga tcatcagtaa aacgccgatg gtcgcgggga
     2941 ttcgcggtcc gaagcgatcg gtgagataac cgct
//
LOCUS       DASULZ010000466         3473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_666338, whole genome shotgun sequence.
ACCESSION   DASULZ010000466 DASULZ010000000
VERSION     DASULZ010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666338"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="VFY96_09515"
     CDS             complement(<1..1015)
                     /locus_tag="VFY96_09515"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF044583.1"
                     /note="catalyzes the formation of malate from glyoxylate
                     and acetyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HEX5606741.1"
                     /translation="MASRVEVGSLNIDERLYRLVRDEIAPGTGVKSDGFWKSLGEIVK
                     ELGPKNRALLAKRDWLQQQIDHWYQERKDRPFNIEEYTDFLKQIGYIVPEGKNFKITT
                     ANVDDEIAAIAGAQLVVPLDNARYALNAANARWGSLYDALYGTNVISQESGAEKGEFY
                     NPRRGAKVIKYTEAFLDKVVGLKSGRFSEVTGFLLKRSGRRKQLAVTLKNGKTVGLAD
                     GRKLIGYQEKAGELTEIVLSNNGSRILIEIDRTHPIGKAHPAGVKDVALESAITTIED
                     CEDAVAAVDAADKVTVYRHWCGLMKGTLETIFEKNGRPLTRKLNPDKVFTTPTGKKLT
                     LPGR"
     gene            complement(1247..2380)
                     /locus_tag="VFY96_09520"
     CDS             complement(1247..2380)
                     /locus_tag="VFY96_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX5606742.1"
                     /translation="MQINIVGAGPAGLLFALLIKRRFPAWQVHVFEQNPPDATYGFGV
                     VFSLNALAFLERDVPDFHAQLLARLESWPIQRIVHRNERVDIDGNGFSAIGRLELNQF
                     LQALCAAAGVEFEYRRPITSATELNDCDLLVGADGANSVLRRSFEKQFEPELTWLTNR
                     FAWYGTRQLFKCLSLTFRANADGFFVAHHYRHSSSMSTFVVECDEATWRRSGLDQMSD
                     ENSRAYCEAVFAPDLAGNSLITNKSVWRQFSLLACRRWSLDHLILIGDALRTVHFSIG
                     SGTRMAFEDAIALDRAFADAGEDVPRALVIFERERRPVVEKILAAATASSYWYERLSE
                     KMRLRPVELAYDYMTRSGRMTDERLRELSPKFMKRIEDERSQS"
     gene            complement(2671..>3473)
                     /locus_tag="VFY96_09525"
     CDS             complement(2671..>3473)
                     /locus_tag="VFY96_09525"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606743.1"
                     /translation="SRAVQTLVGGSIDVAAIGGPAGVDARLAGADTIYVAIPVNRVIV
                     FTVAERQIQRIEEMRGKSIGVTRVGTVTDFFTRVYLRQNGLTPDRDVMIRQMGGLPEI
                     VAGLKAGQIQGGTFGFPAVLHARAAGFHDLVDYNTLGYRYPLSSVIVTQKMLRTQESA
                     IRAFLESQIEAVHRFKTDPNFAMNVIGKYTNTTDRAMLDETQRVYAGAFERIPYPTAE
                     DMKLGITQVAETNPRAKGADPKDFVDARLLREIEASGLVKKLYGEGAK"
BASE COUNT          738 a          989 c          938 g          808 t
ORIGIN      
        1 ttcttcctgg caaagtgagt ttctttcccg tcggtgtggt aaagaccttg tccggattta
       61 atttgcgcgt cagagggcgg ccatttttct cgaatatggt ttcgagcgtc cccttcatca
      121 ggccgcacca atggcggtag accgtgacct tgtcggccgc gtcaacggcg gcgaccgcgt
      181 cctcgcagtc ttcaatcgtt gtgatcgctg attctagggc aacgtctttc acgcccgcgg
      241 ggtgagcctt gccgatagga tgggttcgat cgatttcaat caggatgcgc gaaccgttgt
      301 tgctcagcac tatttccgtc aattcaccgg ctttttcctg ataaccgata agtttgcgcc
      361 catcggccaa gccgaccgtt tttccgttct tgagcgtcac ggccagttgt ttcctgcggc
      421 cggaacgctt gagcaaaaac ccggtgacct ccgaaaacct gccgctttta agacccacga
      481 ccttgtctag gaacgcctct gtatatttaa tgactttcgc gccacgccga ggattataaa
      541 actcgccctt ttccgcgccg ctctcctgcg agatgacgtt tgtgccgtag agcgcgtcgt
      601 aaagacttcc ccagcgcgca ttggcggcgt tcaaagcgta acgagcattg tcgagcggca
      661 cgacgagctg cgctcccgcg atcgccgcga tctcgtcatc gacgttggcc gtcgtgatct
      721 tgaaattctt accttccggg acgatatagc cgatctgttt caggaaatcg gtgtattcct
      781 cgatattgaa cggccgatct tttcgctcct gataccaatg atcaatctgc tgctgcagcc
      841 agtcgcgctt tgccaacagc gcgcgatttt tcggccccag ctctttaacg atctcgccta
      901 atgatttcca aaagccatcg cttttgacac ccgttccggg cgcgatctcg tcgcgcacca
      961 accgatagag ccgctcatcg atattaaggc tacccacctc gactctgctg gccatggtct
     1021 tctccgtttc tagcgcatcc gacatggagc gcagaagttc tgcttaatgg taaacgcggc
     1081 ctatagcgct tcgcaagctt aatgcagttt gctcgtgctc tgccaaagta aaagatctat
     1141 atcacaaact tgtatcgacg cagttgccgg attgcggtcg actgattgaa cgattgcggt
     1201 tcggtggtca tgaccggata ggctcttaag gggttggggt tgccaattaa gattggctgc
     1261 gttcatcttc tatgcgcttc ataaatttcg gcgagagttc acgcagccgc tcgtctgtca
     1321 tgcgcccgct acgggtcata taatcgtaag ccagttccac gggacgaagc cgcatttttt
     1381 ccgacaggcg ctcataccaa taagagctgg cggtcgccgc agcaagaatt ttttcgacca
     1441 ccggccgccg ttcgcgctcg aaaatcacca gcgcacgcgg aacatcttcg cccgcatcgg
     1501 cgaaagcgcg atcgagggcg atggcgtctt caaaagccat gcgtgtgccc gagccgatag
     1561 agaaatgcac cgtgcggagc gcgtcgccga tcaggatcag gtgatccagt gaccagcggc
     1621 ggcatgcgag caatgaaaat tgccgccaaa ccgacttgtt cgtgatcaga gaatttcccg
     1681 cgagatcggg cgcaaagaca gcttcgcagt aggcgcggct attttcgtcc gacatctgat
     1741 ccagacccga gcggcgccac gtcgcttcat cgcactcgac cacaaacgtg ctcatcgatg
     1801 agctgtgacg ataatgatgc gcgacaaaaa atccgtcggc attggcgcga aaggtcagac
     1861 tcagacactt gaacagttgt cgtgtcccat accaggcgaa gcgattcgtc agccaggtaa
     1921 gttctggctc gaactgtttt tcgaagcttc gccgcaaaac tgaatttgca ccgtcagcgc
     1981 caaccagcag atcgcagtcg tttagctcag ttgccgatgt gatcggccgc cgatattcga
     2041 actcgacgcc tgccgctgcg caaagcgctt gtaaaaattg attgagctcg agccggccaa
     2101 tggcggaaaa gccgttgccg tcaatgtcca ctcgctcgtt gcgatgcacg atacgttgga
     2161 taggccacga ttccagacgc gccagcagtt gtgcatggaa gtccggcaca tctcgttcga
     2221 gaaatgccag tgcgttcaaa gaaaaaacta ctccgaaacc gtaggtggcg tcgggcggat
     2281 tttgctcgaa gacatgaacc tgccaggcag ggaaccggcg tttgatcagc agcgcgaaaa
     2341 gcaacccggc cgggccggcg ccgacgatgt taatctgcac gagagttact cacgacgaag
     2401 tgttgcatga agcactgacg gcagcttcgt aacgcgacgc tcgactgagt tcgtcattcc
     2461 cgcatgcttt tggcgggaat ccaggtggaa attcgaactg gacccccgat taaagcattc
     2521 gggggtgacg agtgttgagc gggctattct gttcgagcct tttgagggaa gacatggaaa
     2581 acacgatagt tgatatatca cacttccgaa cttcgtgacc ttcgcgtcct tcgtggtgaa
     2641 tcattctccg tatcccatgt atcccatgaa ttattttgct ccttcgccgt agagcttttt
     2701 taccaagccg ctggcttcga tctcgcgcaa cagacgcgca tccacgaaat ccttcggatc
     2761 ggcgcctttg gcgcgcggat tggtttcggc aacttgcgtg atgccgagct tcatatcctc
     2821 ggcggtcgga tagggaattc tctcgaacgc gccggcgtaa acccgctgcg tctcgtcgag
     2881 catggcgcgg tccgttgtat tcgtatattt gccgatcacg ttcatcgcga agttcggatc
     2941 ggttttgaat cgatggaccg cctcgatttg cgattcgagg aaggctcgaa tcgccgattc
     3001 ctgtgtgcgg agcattttct gagtcacaat gacggacgaa agcggatagc ggtatccaag
     3061 ggtgttgtag tcgaccaggt cgtggaaccc tgcggcgcgc gcatggagga ccgccgggaa
     3121 accgaatgta ccgccttgaa tctggcccgc ctttagtcca gcaacgattt ccggcaaacc
     3181 acccatctgg cgaatcatga catcgcgatc cggcgtcaag ccgttctggc gaaggtaaac
     3241 gcgagtgaag aaatcggtga cggttccgac tcgggtgaca ccaatacttt tcccgcgcat
     3301 ctcttcaatg cgttggattt gccgctcggc taccgtgaaa acgatcaccc gatttacggg
     3361 aatcgccacg tagatcgtgt cggcgccagc aagtctcgcg tcgacacccg ccggaccacc
     3421 gattgccgcc acgtcgatgc tgccaccaac gagcgtctgc acggcgcgtg aac
//
LOCUS       DASULZ010000467         5413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_158882, whole genome shotgun sequence.
ACCESSION   DASULZ010000467 DASULZ010000000
VERSION     DASULZ010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158882"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="VFY96_09530"
     CDS             complement(<1..997)
                     /locus_tag="VFY96_09530"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5606744.1"
                     /translation="METCRAAVITEHNKPLTIERVPIPDLDPGSLLIKIAASTLCGTD
                     VHRWHGPLSDGDSLPIITGHEPCGYVEDIAGERTDILGNPVKRGDRVVWSYVACGSCY
                     YCAVALQPCICPGRASWGHNRSDQHPYLLGSCAEYMYVPKECLIIKVPDEVSSASAAA
                     SACAYRTVMHGFDRLGAIKSHETVVIQGSGPLGNFATAVARDHGAKKVLVIGAPANRL
                     EVTKRMGADAVLNLEEVTDEKQRRQWVRDQTDGRGADIVIQVANNMAVPEGLTLLRGG
                     GRYLNIGAGGKANIAVERLPQEMLFITIRSAEPRHWLQAIDFLASRKKTFPFEEMI"
     gene            complement(1049..2134)
                     /locus_tag="VFY96_09535"
     CDS             complement(1049..2134)
                     /locus_tag="VFY96_09535"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5606745.1"
                     /translation="MSATLSTRIIDGDGHIFEDNSAIIAHMEAPYRDIATRKGIIFPP
                     LDHLHSGRAVETPPQRDQRPPVGPDGWLDFLDDVGIEWTVLYPTTALSYGKIVSLDYA
                     VAVSRAYNDWLYETYVKFNPRFKGMAILPMQDPEEAAKELRRSVTELNMLGAMMPSNG
                     LAHPLGSKFYWPVYEEANRLGCCLAVHGGAHDRFGMDHLNMYVPVHALGHPWGLTINC
                     ADILYNGIFDRFPRVRIAFLEGGIAWLLLLLERLHASHETHFQYIPDGDYGIREGEDP
                     VRYVKKLVDENRFYLGIETEELTMPFAIKTVGNKPFLYSSDFPHEVTHESCKHDIGEL
                     LESNEITEDDKAAMLWRNAETFYKLPL"
     gene            2412..2666
                     /locus_tag="VFY96_09540"
     CDS             2412..2666
                     /locus_tag="VFY96_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606746.1"
                     /translation="MAEETAEEKLARLEAENKALKDQIEQRKPGQLRLKISEKGGLSV
                     YGLGRFPVTLYKEQWKRLLEHAEEIKTFLEQNEQQLKTKQ"
     gene            complement(2702..3637)
                     /locus_tag="VFY96_09545"
     CDS             complement(2702..3637)
                     /locus_tag="VFY96_09545"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5606747.1"
                     /translation="MQQHRSFSCAILSLVIFFGVSFGSQALAQSQDSFAPNRITLGLV
                     SASQQQEITDYFEPFVTYLARKIFAAPGAQGRVLVVSSLPELARLLDQKSVDFYMESP
                     YPTYVVNQVHGAGRVLLRRWKGGLAEYRSLIVSAKDSGIDRLESLRGKQVAFEDPEST
                     SGYFLPKFFFQRNGFKLSPLTDTMPQASYGAIIYLFAGTIDKLVELVLARQVSAGAMS
                     DDDFSSLEPQKKDKLNVLAQTDFLPRHLVSVRKDLSREASERLAAILQGMHDDTGGRQ
                     ILSKLDNTMKFDNLPGGEEAMRRRLLETFFDPQRR"
     gene            3965..>5413
                     /locus_tag="VFY96_09550"
     CDS             3965..>5413
                     /locus_tag="VFY96_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606748.1"
                     /translation="MIVLLVFATLILGGLIFFAFQDNELEANYSRWLLVATLKQALSS
                     PATFLTEELPAGTLLYGGLTLFAVVMTLVFLKMTRDTEVQALRKRLTEIRAEKNETDS
                     LLQEVVWKGKHERQSKDLLNRDLETSIEKIETLILQLNAKEEELKARDVELISLKGSG
                     APGELAGRADGAGAERLLREELKNKDELLQAKDAAVKDLEERLSTKARLWENQLREKD
                     SVLKSRESELGVFRNEISGLTRRLEDSESAKRRAETLLEAELKKTQEVLEANDQAIRA
                     EEKRFAEKVKALESQLSERDKLLRSRETELSGFRQQLTEFELAREQTEARLQEELRKS
                     EFERRAKEQLLVETEQRLNGSLQGLRNSLGEKEVLLQVRDNELASLKSEVKTISLRLS
                     EIAAAKVRVEEALHEELKRTKQQFETEKDGYRRIVEQHEIQVQSLTAELAKRDALLQQ
                     SGTQLEALKREAENLSASLKELSAAKEQSEESL"
BASE COUNT         1344 a         1391 c         1520 g         1158 t
ORIGIN      
        1 tgatcatttc ttcgaacgga aaagtttttt tcctcgaagc gagaaagtcg atcgcctgca
       61 accagtgacg tggttcggcg gagcggatcg tgataaacaa catttcctgc ggcagccgtt
      121 ctacggcgat attcgcctta ccgcctgcgc caatattcaa gtagcggccg ccgccgcgca
      181 gtaaagtcag cccttcaggc actgccatat tgttcgcgac ctgaattacg atgtccgcgc
      241 cgcgcccgtc ggtctggtcg cgcacccatt ggcggcgctg cttttcatca gtgacttctt
      301 cgaggttaag gacggcgtcg gcgcccatgc gtttcgtgac ttcgaggcga ttggccggtg
      361 cgccgatcac caaaaccttt ttggcgccat ggtcgcgtgc cacggcagtg gcgaagttgc
      421 cgagcgggcc gctcccttga atcaccacgg tttcatggct tttgatcgcg ccgaggcggt
      481 cgaaaccatg catgacggtt cgataggcgc aggcggacgc ggccgccgaa gcagaagaga
      541 cttcatcggg aactttgatg atcaagcact ctttaggaac gtacatgtac tcggcacagc
      601 tgccgagcag atacgggtgc tgatcactgc gattgtggcc ccatgaggca cgtcccggac
      661 aaatacatgg ctggagcgca acggcgcagt aatagcatga gccgcaagcg acatagctcc
      721 acaccacgcg gtcgccgcgt ttcacgggat tgcctaaaat atccgtgcgc tcgccggcga
      781 tatcttcgac atagccgcag ggctcatgac ccgtgatgat cgggagcgaa tcgccgtctg
      841 aaagcggacc gtgccagcgg tgcacgtcgg tgccgcaaag ggtagatgcc gcgatcttaa
      901 tcaacaacga gcctggatcg agatcgggaa tgggcacgcg ctcgatggtt aaaggtttat
      961 tatgttcggt gatcactgcg gctcgacagg tttccatcac ggcttcctct atggaacggc
     1021 ttgaaccgtt ttcaacttct tccggccgct aaagcggcag tttgtaaaat gtctcagcat
     1081 tgcgccagag cattgccgcc ttatcgtctt cggtgatctc gttgctttcc aaaagctcac
     1141 cgatatcgtg cttgcagctt tcgtgagtga cttcgtgcgg aaagtcggaa gaatagagaa
     1201 acggcttgtt accaacggtc ttgatggcga agggcatcgt taactcttca gtttcgatac
     1261 cgagataaaa gcgattttcg tcgaccagtt tttttacgta tcgaaccggg tcctcgcctt
     1321 cgcggatgcc ataatcaccg tcgggaatgt actgaaaatg ggtttcgtgc gatgcatgca
     1381 aacgctcgag caacaatagc agccatgcaa tgccgccttc gaggaaggca atgcgcacgc
     1441 gcgggaaacg atcaaatatg ccgttgtaca aaatgtcggc gcaattgatc gtcagccccc
     1501 atggatggcc cagtgcgtgt accggcacgt acatattgag atgatccatg ccgaaacgat
     1561 cgtgagcgcc gccgtgaacg gctagacagc agccaagccg gttagcttct tcgtaaaccg
     1621 gccaatagaa ttttgagccg agcgggtgcg cgagcccgtt ggacggcatc atggcaccaa
     1681 gcatgttcag ctcagtcacg gagcggcgca gctccttggc cgcttcctcg ggatcctgca
     1741 tgggcagaat agccattcct ttaaaccgtg gattaaactt cacgtatgtc tcgtaaagcc
     1801 aatcgttgta ggctcgtgag actgctacgg catagtccag gctgacaatt tttccataag
     1861 agagcgcggt agttggatag agcacagtcc actcgatgcc cacgtcgtcg aggaaatcca
     1921 gccaaccgtc cggtcctacc ggcgggcgct gatctcgctg cggcggagtt tcgacagcgc
     1981 gcccgctatg cagatgatcc agaggtggaa agatgatgcc tttacgggtg gcaatatccc
     2041 gataaggagc ttccatatgg gcgataatgg ctgagttgtc ttcgaagata tgaccgtcgc
     2101 catcgatgat tcgcgtgctg agcgtggctg acattgttac ctcctgcaaa attgagacgt
     2161 ccggaaggtg cactgccgtt ctcttcttat aagctcagtt tcgcggctgg atcaagcgcc
     2221 gaagcgaacg cgctcgtgag gcgtcgtgta cggatgcatt caagccggat gcagctccgg
     2281 ccaatctatt gccgtattga ccggtggaaa tgcgctcgta tatcctggac ctcaaaatga
     2341 gcggctttct ctcccgattc aggtggcgaa gtccgcggcc aattcaaata cggaaacata
     2401 ttggaggggc aatggctgaa gaaactgccg aagagaagtt agcgcgcctc gaggccgaga
     2461 ataaagcgtt gaaggatcag atcgaacagc gcaagcctgg gcagctgcgc ttaaaaatta
     2521 gcgaaaaggg cgggctttcg gtttacggtc tgggaaggtt tcccgtcacg ctttacaaag
     2581 agcagtggaa gcggctgctc gaacatgcgg aggagatcaa gacgttttta gagcagaacg
     2641 agcaacagct caaaaccaaa caatagccgg ccagcaagtc gccggtgatc gcgtgctagc
     2701 gctatcttcg ctgggggtcg aaaaaagtct ccaacagtct gcgccgcatc gcttcctcac
     2761 cgcccggcaa attgtcgaac ttcatggtgt tatccagctt cgacagaatt tgtcgaccgc
     2821 cggtgtcgtc atgcatgcct tgcaaaatcg ctgcgagtcg ttccgacgcc tcgcgtgaca
     2881 aatcctttcg caccgacacg agatgacggg gcaaaaagtc ggtttgtgct agaacgttca
     2941 gcttgtcctt tttttgcggt tccaacgagc taaaatcgtc atcgctcatt gcgcctgccg
     3001 atacttgcct ggcgaggaca agctccacga gcttgtcgat cgttcctgcg aacagatata
     3061 tgatcgcgcc atacgacgct tgcggcatgg tgtcggtcag aggcgatagc ttgaagccgt
     3121 ttcgttggaa aaaaaacttc ggcaaaaagt agcccgaagt ggactcagga tcttcgaagg
     3181 cgacttgctt tccgcgcaga ctctcgaggc ggtctatacc gctatctttg gcgctcacga
     3241 tcaaacttcg atattctgcc aagccgccct tccaacggcg caacagcact cggcctgcgc
     3301 cgtgaacttg attgacaacg tacgtcggat aagggctttc catgtaaaaa tccacgcttt
     3361 tttggtcaag aagcctggcc aactcgggta acgaagagac gaccaaaact cttccttgcg
     3421 cccctggcgc ggcaaaaatc ttccgcgcta gataggtaac aaacggctca aagtagtccg
     3481 tgatctcttg ctgctggctc gcggagacaa ggcccaaggt gatccggttt ggcgcaaagc
     3541 tgtcttgcga ttgggctaga gcctgcgagc caaaggaaac gccaaaaaaa atcactagcg
     3601 acaggattgc gcaagaaaat gatcgatgct gttgcatgct ttagtcaccc tcgatttttc
     3661 ctacttttcc tactcaactg tgttcaggtt ggcaagttta aaatttgcgc acacttaacg
     3721 agtcgttcgc aagctatatc ttgcgcaatt tatacttgga cgggcgcaat ttcgcgtgcc
     3781 aggggatatt tttgggaaca atttgaaaaa cgatgccatt gagacgtgct tagccgatag
     3841 cgaatttcgc tggcaaaatc cgtaatgaag cgtaaaatgg cgggatattt gcggttctag
     3901 ggcctcgcgc cgctccattt cggcacatag gcgccacttt tcctatatgc gaacacgaga
     3961 aaaaatgatc gttctgctgg ttttcgccac tctgattctt ggcgggctga tcttttttgc
     4021 atttcaagac aacgagctag aagccaatta ttcccgctgg cttctcgttg ccaccctgaa
     4081 gcaggccctg tcctcaccgg ctacattttt gacagaggaa ttgcccgcgg gaaccctgtt
     4141 gtacggtggc ttgaccctgt ttgcagtggt catgacgctg gtttttttga agatgactcg
     4201 ggatacagaa gttcaagctc tgcgcaaacg attgacagaa ataagagcgg aaaagaacga
     4261 aacggatagc ctactgcagg aggtagtctg gaaagggaag cacgagcggc aatcgaaaga
     4321 tcttttgaac cgcgacctcg agacgagcat cgaaaaaatc gaaaccctca tccttcaact
     4381 aaacgctaaa gaagaagagc tcaaagcgcg cgacgtcgaa ctgattagcc tgaagggaag
     4441 cggcgcgccg ggcgaacttg ccgggcgtgc cgatggtgcg ggagcagagc gtctgcttcg
     4501 ggaagagctc aaaaataaag acgagctgct tcaagccaag gatgcggcag tcaaggacct
     4561 cgaggagcgg ctgagcacga aagcacggct gtgggaaaat caactacgcg aaaaggatag
     4621 cgtcctaaaa agccgtgaaa gtgagcttgg agtcttccga aacgagatca gtggactcac
     4681 ccggcgactt gaagattcag aaagtgccaa gcgacgagcg gaaactctgt tagaggcaga
     4741 gttgaaaaaa acccaggagg ttctcgaagc caacgatcag gcgatccgtg cggaagagaa
     4801 gcgctttgct gaaaaagtta aagctctgga aagccagctc agcgaaagag acaagctgct
     4861 cagaagccgc gaaacggagc tcagcggttt tcgacaacaa ctgactgagt tcgagttagc
     4921 gcgtgagcaa actgaagccc gacttcagga ggagctgcga aaatccgagt ttgagcgccg
     4981 tgccaaagag cagctcctgg tcgaaacgga gcaacggctc aatggcagcc tgcaaggatt
     5041 gcggaactct ctcggtgaga aggaggtcct gctccaagtc cgtgataatg aactggcatc
     5101 gctcaagtcc gaagttaaga cgatctctct aagattgagc gagatagcgg cggccaaagt
     5161 tcgcgtagaa gaggcgctgc acgaagagct caagcgaacg aagcaacaat ttgagacgga
     5221 gaaagacggt taccgaagaa tcgtcgaaca gcatgaaatt caagtgcaga gcctcaccgc
     5281 cgaattggcg aaaagagatg cgctgttaca gcagagtggc acgcaactgg aggcgctcaa
     5341 gcgtgaggca gagaaccttt cggcgagtct gaaagagctg agcgcagcca aagagcaaag
     5401 cgaagagtca ctg
//
LOCUS       DASULZ010000468         2438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1477840, whole genome shotgun sequence.
ACCESSION   DASULZ010000468 DASULZ010000000
VERSION     DASULZ010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477840"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VFY96_09555"
     CDS             complement(<1..302)
                     /locus_tag="VFY96_09555"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEX5606749.1"
                     /translation="MTTDVTPINLFEFEDVARARLPKAEYDYIAGGATDEISVDRNRR
                     AFASWALRPRVLRDVSVLDLSTVVLGTKVKLPVLIAPCGGHKRAHPDGEVATYRAAA"
     gene            complement(630..1004)
                     /locus_tag="VFY96_09560"
     CDS             complement(630..1004)
                     /locus_tag="VFY96_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606750.1"
                     /translation="MASAEGAASNTDFNLFGGGFGSGAAAGLGGYTNRPEGKVLAGAF
                     ADAYNRLVGAVRSYKAQEVKGGLGTGGQLGVQGGTTPASQALMPASATTTTTTTSPSK
                     PAAKPAPKPAAKPAPTTTKPAY"
     gene            complement(1005..1403)
                     /locus_tag="VFY96_09565"
     CDS             complement(1005..1403)
                     /locus_tag="VFY96_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606751.1"
                     /translation="MAVVEDQTAPWYYRLTREYKLTSTVPLIRLLVQQSNCFVVVERG
                     RAFGQMQGERALQQTGELRQGSNFGQGQIVAADYSLNPMIRFSQKDTKGLGGALAVIP
                     YAGAFGALAGGVRTREASTTLTLVDNRSGI"
     gene            complement(1701..>2438)
                     /locus_tag="VFY96_09570"
     CDS             complement(1701..>2438)
                     /locus_tag="VFY96_09570"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606752.1"
                     /translation="YVRKALYGDPYVHIAQSNNAWRENFLVCGKGVSGLQDLKGKRVA
                     MDDYDGHTGLNVWLYLKQHGLEEGRDVELVTDPVKGAERAKAVSEGKYDATFIRAVDR
                     LRALKFGAKIVEVPPMAMIEGVTLTTTTTYVSKHEDECRSLIMALIDGIHFFKTNKAD
                     TLAIVKKHCSQLLKMHDDEEWNCFYDTQSESLEKAPYPSLEAIQNVFALGVKRDPQIS
                     EFNPLALWDLHYVKEIDDSGYIRSLYR"
BASE COUNT          500 a          731 c          644 g          563 t
ORIGIN      
        1 gccgcggccc ggtacgtggc gacctcgcca tcgggatgcg cccgcttgtg accgccgcag
       61 ggcgcgatca aaaccggcag cttcactttc gtccccagaa ccacggtgga caaatccaag
      121 acgctgacat cccgcaagac gcgcggccgc agcgcccagg aagcgaatgc gcgccggttg
      181 cgatcgacgc tgatctcatc cgtggcgccg cccgcgatat agtcgtactc cgccttcggc
      241 aaacgagcgc gggcaacgtc ttcgaattca aaaagattga tgggcgtgac atcggtcgtc
      301 attcttcctc cggcagcttg tcagtgatca accttgatca cccaagtagc caaatcgtga
      361 gctcaaggct agttcatatc ttttctcgca ctatgcgtcc agctgcgtta atgaaagggc
      421 ggctgaacca gttttaaagc ttggggagtg catgcgtgtg tgattctgag atgacagctg
      481 gcatcctcaa ttcacgctgc gggagaattc ttcgtttcaa caagccacga ctatgtataa
      541 ccctccggaa gttacctcgc agttaacgca aaaacgcccg gtgaggagtt ttcccaccgg
      601 gcgctggtac actgatctaa agacgttgct cagtaagccg gcttggtagt ggtaggcgcc
      661 ggctttgccg caggcttcgg tgccggcttc gccgcgggct tggacggcga agtcgttgtt
      721 gtggtcgtcg tcgcagaagc tggcatcaag gcttgcgatg ccggcgtcgt acccccttgt
      781 acgcccaatt ggccgccggt accgagaccg ccctttactt cctgagcttt gtaactcctg
      841 accgcgccaa ccagccgatt gtatgcatcc gcgaatgcgc cggcgagtac cttaccctct
      901 ggcctattcg tgtagccacc gagtcccgcg gctgctccgg aaccaaagcc accaccgaac
      961 agattgaagt ccgtgttcga tgccgcgcct tcggcggaag ccaactatat gcccgatcta
     1021 ttgtcgacca atgtgagcgt cgtggatgct tcgcgtgtcc tcacccctcc cgcaagcgca
     1081 ccgaacgcgc ccgcatacgg aatcacggcc agcgctcctc ccagcccctt cgtgtccttc
     1141 tgactaaaac gaatcatggg attgagcgag taatcggcag ctacgatttg gccctgcccg
     1201 aagttgctgc cctgacgcag ctcgccggtt tgttgaagcg ctcgctcccc ctgcatttgg
     1261 ccaaacgcgc gaccacgctc gacaaccaca aagcagttcg actgttgaac cagcaaccgg
     1321 atgagcggaa ccgtcgaagt cagtttgtat tcacgagtaa gccgataata ccaaggagcg
     1381 gtttggtctt cgacgactgc caacgttccc agcgattggt cacagcgctc gagcttggca
     1441 tttgcgttct ggctactcgc ccccctgctg agccggtgac cggcgtgcgg gcggaatcgt
     1501 cgcccatttg gacgccgcaa ccagcaagca aaacggctgt taatagaccg gaaaattcct
     1561 taacgagaag cgctttcatg tttcctccaa ggtgcaaatt ttcacttctc aaaagcagcg
     1621 cggctctttt accttttttt acgctttccc cgtcaatatc tcctgagaat catcgaattt
     1681 tacccaatcg cgacagccac ttagcgatag aggcttcgaa tataaccgct gtcgtcgatt
     1741 tctttgacat agtgcagatc ccagagtgcg agtggattga attcgctgat ttgcggatcc
     1801 cgtttcacgc cgagagcgaa aacattctgg atcgcttcga gggatggata aggcgctttt
     1861 tcgagcgatt ccgattgagt gtcgtagaaa caattccact cttcatcgtc gtgcatcttg
     1921 agcagctgcg agcaatgctt ctttacgatg gcgagcgtgt cggccttgtt ggttttgaaa
     1981 aagtgaatgc cgtcaatcag cgccatgatc agtgagcggc attcgtcttc gtgcttgctc
     2041 acataggtcg tggtggtcgt cagggtcacg ccctcgatca tcgccatagg cgggacttcg
     2101 acgatttttg ccccgaactt gagcgcgcgc agtcgatcca ccgctctaat aaatgtcgca
     2161 tcgtatttgc cttcgcttac ggccttggcg cgctccgcgc ctttcaccgg atcggtcacc
     2221 aactcgacat cgcggccctc ttcgagtccg tgctgtttga gatagagcca gacattcaac
     2281 cctgtatgac cgtcgtaatc gtccatcgcc acgcgcttgc cttttaaatc ctgcaagcca
     2341 ctgacaccct tgccgcagac gaggaagttt tcgcgccacg cattgttgct ctgggcgatg
     2401 tgcacatagg gatcaccgta aagcgccttg cgaacgta
//
LOCUS       DASULZ010000469         2469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_463616, whole genome shotgun sequence.
ACCESSION   DASULZ010000469 DASULZ010000000
VERSION     DASULZ010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463616"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..416)
                     /locus_tag="VFY96_09575"
     CDS             complement(42..416)
                     /locus_tag="VFY96_09575"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX5606753.1"
                     /translation="MKIWKLKSPPSEHDLLFATVEGKPLHQKAATAIIDLVIIAAEVK
                     RLTPQKFRHTFASLLLSRNVPITKVNFWGNRDPTITLRVYAHFIEDKKEGVQDLASSI
                     CHEILTRRDAIKIFNKRRQIIQ"
     gene            complement(460..669)
                     /locus_tag="VFY96_09580"
     CDS             complement(460..669)
                     /locus_tag="VFY96_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606754.1"
                     /translation="MKKLINATAAGSLDRVVIMVPALAGLRLGEVLGLQSPAVDLKKN
                     KLHVRTNVIDTGKANGGRGLGAPNR"
     gene            complement(692..853)
                     /locus_tag="VFY96_09585"
     CDS             complement(692..853)
                     /locus_tag="VFY96_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606755.1"
                     /translation="MTSANTANKALTTWTIFKLAQRYGPLQGKANAAEVAERIKISND
                     TAIDEQVTP"
     gene            complement(906..1196)
                     /locus_tag="VFY96_09590"
     CDS             complement(906..1196)
                     /locus_tag="VFY96_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606756.1"
                     /translation="MAINKTVAGTYRVDLRDQTGRRLRNTFDRFEAARAYNKQALGDI
                     SKGDFVAPSDVTVKDMAESWHKRKKDAGGHGAATLQNWRTHIENTSCRRSAN"
     gene            2172..2366
                     /locus_tag="VFY96_09595"
     CDS             2172..2366
                     /locus_tag="VFY96_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX5606757.1"
                     /translation="MDIVKRLLVEEDGQGLVEYTLIVVLVALVFWVAIKNTNVGDQLA
                     SGWSKVVSCVGTPASCDSSS"
BASE COUNT          514 a          664 c          618 g          673 t
ORIGIN      
        1 caaaagagcg gtcgaatcgt ccgctgaata gaccggttca atcattgaat tatttgtcgg
       61 cgcttgttga agattttaat ggcgtcacgc cgcgtcaaaa tctcgtgaca gatactagat
      121 gcaagatcct ggacgccttc ctttttgtcc tcgatgaaat gcgcgtaaac tcttagcgtg
      181 atcgtcggat cgcggttgcc ccagaagttt accttcgtaa tgggaacgtt gcgcgagagc
      241 aatagtgacg cgaacgtatg ccggaacttt tgcggtgtca gccgtttgac ttcagccgcg
      301 ataatcacga gatcaataat cgccgtagcg gctttttggt gaagcggctt accttctaca
      361 gttgcgaaaa gcagatcatg ctcactaggc ggacttttca atttccagat tttcaatcct
      421 tcgccaattc gtgcagcaaa tccagcacac gtcgactgct catcgatttg gtgcgccaag
      481 tcctctgccg ccgttcgcct taccggtatc gatcacattg gttcgtacat gcagcttatt
      541 cttcttgaga tcgacagcag gcgattgcag tcccaagact tcgccgagtc gcaacccggc
      601 tagagcgggc accatgatca cgactcgatc aagactgccc gccgccgtcg cattgatcag
      661 ctttttcaat tcctctgtgt tgtaaacgca ttcagggcgt gacctgctcg tcaatcgcgg
      721 tgtcgtttga aattttgatc cgttccgcaa cctcggccgc atttgcctta ccctgaagcg
      781 gcccataacg ctgagcgagt ttgaaaatcg tccaagtggt cagcgcctta ttggcggtat
      841 tggcggaagt cattttagcc catgccgccg cggtttcgat ctgctagccc agcttagctg
      901 gattctcaat tcgccgagcg acggcacgat gtattttcaa tatgggttct ccagttctga
      961 agagttgccg ccccatgacc gccggcgtct ttcttccgct tgtgccagga ttccgccatg
     1021 tctttgaccg tcacgtcgga tggcgcgacg aaatcacctt ttgaaatatc gccgagcgct
     1081 tgcttgttgt acgcgcgggc agcctcgaac cgatcaaagg tattgcgtaa tcgccttcca
     1141 gtttgatcgc gcaaatcgac gcgataagtc ccggctacgg tcttgtttat ggccatggat
     1201 ttctggtgtt ttgatttcct gtgttatatt tcctgcgcgc tgtgccgacg gtcactgatc
     1261 ttgcgaagac ctattctccg gatctcccaa cctcctcccc ggcatgtgcc gaacgcgatc
     1321 tctggagcgc cgtccttgca ctcgctgtgc acgacttgaa atcatgtgaa cagctggacc
     1381 gtcaatcagc acacacctgg attttttccg attcgataca cgtcggtagc gttcatctgg
     1441 atctgtttat catatcggag ttgagccgtc agcgatgtgg gggatgattg cgcaccgagt
     1501 cgctgaaacg ctcggttaat acatgtttgt atcgctcatt ttgctcatat ttatccgccg
     1561 gcattttgct tcgtttctag cttcgagaga atcttgatgt gctgtcgttc tgaggtttct
     1621 ttaaaaagct ttccggatgg aatgccaagc ccctttagtc ttgctagcct agcgagccgt
     1681 tggtcgctct cgtaccgggc ggttacaagt tcctggtaac ttgatccaac agcaaacggg
     1741 cgatctccaa cccaacacac tcggcgatac tcttgtcatc gcacgtcatc ggaaccgaca
     1801 cctggcgagc tctgtaacca ctccattgct gtgcttgaac gcgatgcggc cggttggctt
     1861 ccatcccgtg acgccgccga gcggtgtttc gctgaccggc tcattgccgc cataaagttt
     1921 gaagccgcga attcttcgtt cattgacttt tcttccaacg tttttgtttg ccgggcgctg
     1981 gctcaacaaa tttgactgta tcctgatgtc cgggcaaaaa tgcgtctgac aatgcctgcc
     2041 ctcatattcc cagaagtcgc aagaatctcc tatagcgttc atacgagcgc agggcacaat
     2101 ctatgctcaa agagtggctc ccgagcccac atgaaacttt tccttgcgaa cggaaggagg
     2161 tgaaatcatt aatggacatc gtgaagcgtt tgttagtcga ggaagacggc caaggcttgg
     2221 ttgaatatac attgattgtg gttcttgtcg cactggtctt ctgggttgct atcaaaaata
     2281 ccaacgttgg cgatcagttg gccagcgggt ggagcaaagt tgtatcttgc gtaggtacgc
     2341 ctgcgtcttg cgattcttct tcgtaaactc ttcgactcgc tcggatgccg gagggtagct
     2401 acgattactt ccggcatcca agccggcgcg gcaaaatgat catctcaaca gtgaaagcca
     2461 gccaggtcc
//
LOCUS       DASULZ010000470         7191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_666766, whole genome shotgun sequence.
ACCESSION   DASULZ010000470 DASULZ010000000
VERSION     DASULZ010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7191
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666766"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="VFY96_09600"
     CDS             complement(<1..1076)
                     /locus_tag="VFY96_09600"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606758.1"
                     /translation="MNRRRFTKLALGSTVATMVAPFNILRAQSNKLKVGVLLPKSGLQ
                     GLIGQSCQKGAGLAPAVIRDWLGIEIELMNADTETNIDTARTRAERLIQDGAHCLIGP
                     FDSGAAGAIAQVAEQHGMPFVINIAAAPQITEQGYKFVFRNFPVAAELVKNGLTLIDD
                     LFRATKMSPRTAVFMHVNDTFGQANAKAIAALMPQLNLPFRLVETISYDPAAKDLTVE
                     VTKAKTTRADFLLLVCRLNDAIMLRREIVKQRWNTMGIVSPGSPGMYESQFFRALGKL
                     SDGCISNVPWFDPKSALTKAVEAAFKKQNPKDQLRYHALNVGYTFEAILIAADAFKRA
                     RSTHAQPLTEAIRKTEITNRMMIGG"
     gene            complement(1345..2775)
                     /locus_tag="VFY96_09605"
     CDS             complement(1345..2775)
                     /locus_tag="VFY96_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007753521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX5606759.1"
                     /translation="MAAEQNPFAKIPAGLSTTAAAAAFIESVNYAAIPSDAVRIGTRC
                     LLDGLGLFVAGSEEHSVEIIADEAAHTGGRPDALLLGRGSTKVPAPIAARVLGTAGHA
                     HDWDDSQVSLDPAHVYGLLTHPTIPPLTSALVLAQKMGAVNGKTFMLAFLVGFEVECK
                     ISEWMLPQHYLRGMHSSGTVGTFGAYAAAAKLLGLRGDPLRSGFGIAASLAAGIRCNF
                     GTMTKPLHVGRAAENGVTAALFAARGFTADPSALDGPWGFYAVHGGGVSAEKVSQEFG
                     RVWSIVDPGVSIKPYPCGVLTHPTIDLMLKLVTEAGVQPDDIVSVHVYAGSNILNPIR
                     YPIAANHLQAKFSLPAALAMIALARKAGKREFSDAFVASPEMQDMQKKITAELDPEIE
                     KLGFDKMRSRIVLRLKNGQQLEGWADEHYRGGPQNPLSDTELEAKVRSCCEGILKETA
                     QSELIDTARSITALSDVTRLMEIING"
     gene            complement(2860..3540)
                     /locus_tag="VFY96_09610"
     CDS             complement(2860..3540)
                     /locus_tag="VFY96_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX5606760.1"
                     /translation="MLDPLDALKEKMVLVCKALQKQRLLDGYGHVTARLPDGRILSTP
                     HMPPGKVSLRDLIIIDGQGNKLEGAGEPNGETPMHTSIYKARSDVQCILHYHPDELIV
                     VAASGQEIKVIANCGVHFYRGTPIYDSPLLIRNHTLGDKVAATLSDRTAVLLRGHGGT
                     VVASDLDMLLRRGIDFVKSAKIQIMAAPLGHVKTHSKEECEQMAAGHDRADASRRHVD
                     FYISEIFE"
     gene            complement(3700..4539)
                     /locus_tag="VFY96_09615"
     CDS             complement(3700..4539)
                     /locus_tag="VFY96_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5606761.1"
                     /translation="MPFAKIRGININYQILGESGPWVALSPGGRRDMSGIETQAKALA
                     ALRHRVAIFDRRNCGASDVVIDGNESEYEIWADDVHELLSQLGALPAFVGGSSSGCRT
                     SLLFALRHPSSVCGLLLWRVTGGRFACERLAEEYYGQYIKAAEKGGMAAVCEMEHWRE
                     RIQARPENREALMRLTPARFIAVMSHWREYFLKGIDLPVIGATEQELQSIKVPACIVP
                     GNDNTHGRQTGETLGRLIPKSEVHILFPKHYDEPLSPREEWDEKAGEMAALFADFIKR
                     SAA"
     gene            4957..6210
                     /locus_tag="VFY96_09620"
     CDS             4957..6210
                     /locus_tag="VFY96_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5606762.1"
                     /translation="MRIAQVAPLYERVPPVCYGGTERVVSYLTEALVEQGHEVTLFAS
                     GDSLTQAKLISPCAQSLRLNPDCIDDLAYNLLQLEQVFQRADHFDIVHFHIDYFHYPF
                     SRRQKVPHLTTLHGRLDLADLVPLYREFDDMPVVSISNSQRAPLPWIRWCGTVYHGLP
                     VDLYKSKKERGRYLLFLGRISPEKRPDRAIEIAKRAGMPLKIAAKVDEKDRKYMENEI
                     RPLLDHPLVEFLGEIGDSGKEELLRNAYALLFPIDWAEPFGLVMIEAMACGTPTIAFR
                     GGSVREIITDGLTGYVVETVEHATEALKALDTFDRMRCRSIFEERFSARRMAQDYLQI
                     YQRLIYTDARDTRRRSIDKPLLRPATIELRRKTSNGAILDSAGSNQSRFLITERAQAS
                     RELGNGQHRRGTDTPAKHERARDTE"
     gene            complement(6106..>7191)
                     /locus_tag="VFY96_09625"
     CDS             complement(6106..>7191)
                     /locus_tag="VFY96_09625"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5606763.1"
                     /translation="TNSPFQLGLTGLFRALPVMILSLPGGVLADRMDRRKLLIMTQSL
                     AALLSLTLGALTSAGTIEVWQIYAVTFLSGAVGIFDAPARTAMIPALVSADQLASAYA
                     LNITWRQIATLAGPFVGGIVLSAWGISQAYYLDAASFVAVIICLASMQRRVKPAVERK
                     ESPFESVRAGFLFIRESSVILALMAMDTCVQFFGAYRSMMPVFARDILGSGPSALGLL
                     LGVPALGALAGSGVILATGNPRRKGQLIISVTLLYTVGLICFALSRSLVLSLAIVFCL
                     GLVDAVGETLRDTLVQLTTPDAMRGRVKSFDQVFMSAGTYLGHAQMGAAASLLGVPGA
                     LVLGGCIGSAAVLTIAKLSRSLRALSN"
BASE COUNT         1595 a         1992 c         2018 g         1586 t
ORIGIN      
        1 ccaccgatca tcatgcggtt tgtgatttcc gtttttcgaa tcgcctcggt gagcggctgc
       61 gcatgggtcg atctcgcgcg cttgaaggca tcggcggcga tcagaattgc ttcgaatgta
      121 tagccgacgt tcagagcatg atagcgcaat tgatctttag gattttgttt tttaaatgcg
      181 gcctccaccg ccttggtgag agcggatttc ggatcgaacc aaggcacgtt ggaaatgcaa
      241 ccgtcggata gcttgccgag agctctaaaa aactggctct cgtacatgcc cggcgatcca
      301 ggactcacga tacccattgt gttccaccgc tgtttgacga tctcacggcg cagcataatc
      361 gcatcgttca gccgacacac aagaagcagg aagtccgctc ttgtcgtttt ggctttagtg
      421 acttcgacgg ttaaatcttt ggccgccgga tcataagaaa tcgtctccac gagccgaaag
      481 ggcaagttga gctgcggcat cagcgcggca attgccttag cattggcttg gccaaaggtg
      541 tcgttgacgt gcatgaacac cgccgtgcgc ggcgacattt tcgtggcgcg gaagagatca
      601 tcgatcaatg taaggccgtt tttgactagc tcggcggcga cgggaaaatt acgaaaaacg
      661 aacttgtagc cttgctcggt gatctgtggc gcggcagcga tattgatgac gaaaggcatg
      721 ccatgctgct cggcgacctg cgcgattgcg ccggctgccc cggagtcgaa cggtccgatc
      781 aagcaatgcg cgccgtcctg gatcaggcgc tcggctcgag tgcgcgctgt atcgatattg
      841 gtttccgtgt ccgcgttcat cagctcgatc tcgatcccga gccaatcacg aatcaccgcc
      901 ggcgcgaggc ccgcgccttt ttggcacgac tggccgatca ggccttgcaa gccgctcttc
      961 ggcagcagca cgccgacttt caatttattg ctttgcgcgc gaagaatatt gaacggcgcg
     1021 accatagtag cgacagtgct gccgagggcg agcttggtaa atctccggcg gttcatgaac
     1081 aattaccgac agtggttgtt tcgtccgtac gatgattttt cgcaggttca ccgcagagta
     1141 cgaagacctg tcgacctgat gacgcgagtc tcacaaggta tcatttcgac cgcttcgact
     1201 tcgctccaga tagagtccgg cgagaatctt tctgccgtgc atcgaaagcg agcgattaga
     1261 aaatttctcc cttcggtcga aatgacatct cggtagcgtt tcactattgc gcgatttagg
     1321 tacgtacgct ttttccgctc caggtcaccc gttaatgatc tccatcaatc gggtgacgtc
     1381 ggaaagtgct gtgatcgaac gcgctgtatc gataagctcc gattgcgctg tttctttcaa
     1441 aatcccctcg cagcaagagc gcactttggc ttccagttcc gtatcactca aaggattttg
     1501 cggaccgccg cggtagtgct cgtcggccca gccttcgagc tgctgaccgt ttttgagccg
     1561 aagaacgatg cgcgaacgca ttttgtcgaa acccaatttt tctatttcgg gatccagctc
     1621 agcggtgatt tttttttgca tgtcctgcat ttcaggcgaa gcgacgaacg cgtcggaaaa
     1681 ttcgcgcttt ccggctttgc gggcaagcgc gatcatcgct agcgcggcgg gcagagaaaa
     1741 ctttgcctgc aaatggttgg ctgcaatggg atagcgaatc ggattcaaaa tattgcttcc
     1801 ggcatagacg tgaacgctca cgatgtcgtc aggttgtacg ccggcttcgg tcacgagctt
     1861 gagcatcaag tcgatggtcg gatgcgtgag gactccgcac ggataaggtt tgatggaaac
     1921 gccgggatcg acgatgctcc agactctgcc gaattcttgc gaaaccttct cggcgctcac
     1981 gcctcctcca tgaaccgcgt agaagcccca cgggccatca agggcgctcg gatcggccgt
     2041 aaacccccgg gccgcaaaca acgcggcggt cacgccgttt tcggctgcgc gacccacatg
     2101 gagcggcttt gtcattgtgc caaagttgca gcgaatcccg gccgccaagc tggccgcgat
     2161 accgaagccg ctgcgcaatg ggtcgcctcg taagccgagc agcttggccg cggcggcgta
     2221 agccccaaaa gtgcccaccg taccgctcga gtgcattccc ctaagataat gctgcggcag
     2281 catccattcg gatattttgc actcgacttc aaaaccaacg aggaaggcga gcatgaaggt
     2341 tttaccgtta acggcgccca tcttctgcgc gagtaccaaa gcgctggtca acggcggaat
     2401 cgtcggatgg gtcaaaagcc cgtagacatg cgccgggtca agactcactt gactgtcatc
     2461 ccaatcgtgc gcgtggccgg cggtgcctaa aacacgagcc gctataggag caggcacttt
     2521 cgtcgagccg cgcccaagta aaagcgcatc cggcctgccg ccggtgtgcg cggcttcatc
     2581 ggcgatgatc tcgaccgagt gttcttcgga cccagcgacg aacaggccga ggccgtccag
     2641 taagcagcgg gtgccgatgc gcacagcgtc cgacggaatg gcggcgtaat ttacactttc
     2701 gatgaatgcg gcggctgccg ccgtcgtact caatcccgct gggattttgg caaaaggatt
     2761 ttgttcagcg gccatgaatt gagtctcacc ataactagtt ttcgcatcgg tagcttgtcg
     2821 gcgctagctt cgtctcggca aagatttagc gccagggtgt cattcaaaaa tttcggaaat
     2881 gtaaaaatca acgtggcggc ggcttgcatc agcccggtcg tgaccagcag ccatctgctc
     2941 acattcctct ttgctatgag tcttgacgtg cccgagaggc gcagccatga tttgaatctt
     3001 cgccgacttt acgaaatcga tgccgcgccg aaggagcata tcaagatcac tcgcgaccac
     3061 ggtaccgcca tggccgcgca gcagcacggc ggttcgatcg ctgagagtcg cagcgacttt
     3121 gtcgcccaga gtatgattgc ggatcaataa tggcgaatcg tagatcggtg tgccgcggta
     3181 aaagtgaacg ccgcaattgg cgatgacttt aatttcttgt ccggaagcgg ccacgacaat
     3241 caattcgtcg gggtggtaat gaagaatgca ctgtacatcg gaacgagcct tatagattga
     3301 ggtgtgcatt ggggtttccc cgttgggttc tccggcgcct tcgagcttgt tgccttggcc
     3361 gtcgatgata atcagatcgc gcagcgaaac ttttcctggc ggcatatgcg gcgtgctcaa
     3421 aatacggccg tcgggaagac gcgcggtgac gtgcccgtag ccatcgagca accgctgctt
     3481 ttgtagtgcc ttgcagacca gcaccatctt ttccttgagc gcatcgagcg ggtcgagcat
     3541 ggagttctcc ttgagaccgg gtcgatttca cttcgctggg taagtgaatg ctgcgccgaa
     3601 attcgtcatt cacgcgagcg acgagcgtta ttcgatgaat ggaaatcaga cccgagcaaa
     3661 ttatttgagt ggcggcttaa gggaaacgtt gttatttagc tatgccgccg accgtttaat
     3721 aaaatcggcg aacagtgccg ccatctcacc ggctttttca tcccattcct ccctcggact
     3781 cagaggttca tcatagtgct tcggaaacaa gatatgcact tcgctttttg gaatcaggcg
     3841 gccgagagtt tctccggtct ggcggccatg ggtgttgtca ttgccgggaa cgatgcaagc
     3901 gggtactttg atcgactgca attcctgttc ggtggccccg atcactggaa gatcgatgcc
     3961 tttgagaaag tattcgcgcc agtgggacat gaccgcgata aaacgtgccg gcgttagtcg
     4021 catcaacgcc tcgcggttct ccggacgcgc ctgaatgcgc tctctccagt gctccatttc
     4081 acagacggca gccatgccac ctttttccgc ggctttgatg tactgaccgt aatattcctc
     4141 agctaatcgc tcgcacgcga aacgcccgcc ggtgacgcgc caaagcaaaa gcccgcatac
     4201 cgacgagggg tgtcgcaggg cgaatagcaa cgatgtgcgg cagcccgacg agctgccgcc
     4261 cacaaacgcc ggtaaggcgc caagctgcga tagcaactcg tgaacatcat cggcccaaat
     4321 ttcatattcg gattcgttcc catcgatcac cacgtcggaa gcgccgcagt tgcgtcggtc
     4381 gaagatggcg acacggtgcc gaagcgctgc gagcgctttc gcttgggtct cgatgccgct
     4441 catatcgcgc cgtccgccgg gggagagcgc cacccatgga ccgctctcac cgagaatttg
     4501 gtagttgatg ttaatgccgc gaatttttgc aaatggcata tcgtgctccc cccgactgat
     4561 gctgttcgaa cgaccattgt ctgtagcata ctcgtatcat cgagtacaat ttgaagccga
     4621 catccagaga ggttgctgtg gataaattca gacgagttga aggaagagtt gtgagaaaaa
     4681 cgaacacgcc gttcgcgcat cgtcgcaaac tacaaacatt tttctggcat gagggttgct
     4741 gcgtgggatt tatgcagcga ttcatccaat tgagcaaggt cgtcgaagac aaaatgtaaa
     4801 atagtttcac ttaatcttct gcttggcata acagcttcga ttcccagcgc ggcttttggc
     4861 cgcccacccc gttgttttct tccgatctgc ttatcgtttc gacagctccg cctgcgctgc
     4921 atgtaagtgt tcgcagcaaa tctggaagga aagatcatga gaattgctca agtcgcaccg
     4981 ctctatgaac gagttccgcc ggtttgttac ggcggcacgg agcgtgttgt ttcctattta
     5041 accgaagcgt tggtggagca gggccatgaa gtgacgcttt tcgcaagcgg tgactcgttg
     5101 actcaggcaa agctcatttc tccctgtgcg cagtcactcc gcctcaaccc tgattgtatc
     5161 gacgacttgg cttataattt attgcaactc gagcaagtgt ttcaaagggc agatcacttc
     5221 gacatcgtcc attttcatat cgactatttt cactatccgt tttcgcggcg gcaaaaggtg
     5281 ccccatttaa ccacgttgca cggtcgactg gatctagccg atctagtgcc gctctaccgg
     5341 gagttcgacg atatgccggt agtttcaatt tccaactcgc agcgcgcacc actgccgtgg
     5401 atacgttggt gcggcacggt ctatcacggc ctgccggtcg atctttacaa atcgaaaaag
     5461 gagcgtgggc ggtacctttt gtttctaggc agaatttccc cggagaaacg tccggatcga
     5521 gcgatcgaga tcgccaagcg cgcgggcatg ccgctcaaga tcgcggctaa ggtagacgaa
     5581 aaagatcgca agtacatgga gaacgaaatt cgtccgctcc tcgaccatcc gctggtcgag
     5641 tttttgggcg agatcggcga ttccggtaaa gaagagcttt tgcgcaatgc ctacgcgctg
     5701 ctctttccca tcgactgggc ggagcctttt ggtttggtca tgatcgaggc gatggcgtgc
     5761 ggcacgccga ccatagcgtt tcggggcggc tctgtgcgcg aaattatcac tgacggactt
     5821 accgggtatg tcgtcgaaac ggtggagcat gcgaccgagg cattgaaggc gctggacaca
     5881 ttcgaccgca tgcgctgccg aagcatcttc gaggagcgct tttcggcgcg ccgcatggcg
     5941 caagattacc tgcaaatcta tcagcggctg atttatacag acgcgcgaga tacgcgccgg
     6001 cgcagtatcg ataagccttt gttaagaccc gcaacgatcg agctacggcg gaaaaccagc
     6061 aacggagcga ttctcgatag tgccggatca aatcagtcga ggtttttaat tactgagcgc
     6121 gcgcaggctt cgcgagagct tggcaatggt cagcaccgcc gcggaaccga tacacccgcc
     6181 aagcacgagc gcgcccggga caccgagtag gctcgctgcg gcgcccattt gcgcatgtcc
     6241 aagataggtt ccagctgaca tgaagacctg atcgaaactc ttgacccgcc cgcgcatcgc
     6301 atcgggtgta gtcaactgta cgagagtatc tctcaacgtc tcgcccaccg cgtccactaa
     6361 ccctaaacag aaaacgatcg caagcgacaa caccagagag cgtgatagag caaaacagat
     6421 cagacccacg gtatagagca acgtgacgct gatgatgagc tggcccttac ggcgagggtt
     6481 gccggtggct aggattactc cagagcctgc gagggcgccg agcgccggta cgcctagcaa
     6541 caaaccaaga gccgacgggc ccgagccgag aatgtcgcgc gcaaataccg gcatcatcga
     6601 gcgataggcg ccgaagaact gtacgcaggt atccattgcc atcaaagcca aaatgacgga
     6661 gctttcgcgg ataaagagaa accccgcgcg tacgctttcg aagggcgatt cctttctttc
     6721 cacggcgggc ttgacgcggc gctgcatgga cgcgaggcag ataattaccg cgacaaaact
     6781 cgccgcgtcc agataatagg cctggctgat accccaggcg ctcagcacga ttccacccac
     6841 aaatggtccc gcaagagtcg cgatttgccg ccaggtgata ttgagagcgt aagcggacgc
     6901 gagttggtcg gcggatacga gcgctggaat catggcggtt cgtgctggcg catcgaagat
     6961 gcctacggcc ccggacagaa acgttacggc gtagatctgc caaacctcaa ttgttcccgc
     7021 cgaagtcaaa gcacccagag tcaaagataa cagcgccgcc aggctctgcg tcatgataag
     7081 aagcttgcgc cggtccatgc ggtcagccag gacacccccc ggcaacgaca gaatcattac
     7141 gggcagcgcc cgaaagagac cggtcaagcc aagttggaac ggagagttgg t
//
LOCUS       DASULZ010000471         2349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1071307, whole genome shotgun sequence.
ACCESSION   DASULZ010000471 DASULZ010000000
VERSION     DASULZ010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071307"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VFY96_09630"
     CDS             <1..347
                     /locus_tag="VFY96_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606764.1"
                     /translation="VNAKKAVDLPNVANAIDYAKTNEARQLIDAGVHATSAILRAYAL
                     PPGTPKDRLNLLRKSFMDTLKDPDFVAELKKADLDLNPVTGADVEATVHKLFQLDSGM
                     VTKLKEILVPKK"
     gene            410..985
                     /gene="gfa"
                     /locus_tag="VFY96_09635"
     CDS             410..985
                     /gene="gfa"
                     /locus_tag="VFY96_09635"
                     /EC_number="4.4.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007802638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051907 - S-(hydroxymethyl)glutathione
                     synthase activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione synthase"
                     /protein_id="HEX5606765.1"
                     /translation="MPDKISIHPKIDSGIQPAAKDFAGGELVCQCPSEPVRVTVNAQS
                     AHNHACGCTKCWKPKGALFSMVAVVPRDKLKVTANANKLVVVDASAAIQRYACTECGV
                     HMYGRIENAKHPFYGLDFIHTELSPQSGWSPPEFAAFVSSIIESGVDPAQMGAVRARL
                     RELGLEPYDCLSPALMDFIATHVAKAAGVLR"
     gene            complement(1081..1578)
                     /locus_tag="VFY96_09640"
     CDS             complement(1081..1578)
                     /locus_tag="VFY96_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5606766.1"
                     /translation="MPLMQKITPCLWFDDQAEQAAEFYTEIFKNSKIKKISRYGKEGY
                     EIHHRPEGSVMTVEFELDGQVFTALNGGPVFKFNEAISFQVDCTSQDEVDYYWEKLSE
                     GGDEKAQQCGWLKDKYGLSWQIIPRALVEMLNDPDATKSQRVMAAMLKMKKIEIETLK
                     RAYAG"
     gene            complement(1610..>2349)
                     /locus_tag="VFY96_09645"
     CDS             complement(1610..>2349)
                     /locus_tag="VFY96_09645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX5606767.1"
                     /translation="LGNAAKYTPENGRIQIRVSRDADNALVSIVDNGIGITEESLHHL
                     FDLFYQAQPPLESIASGLGIGLTLARRLIELHGGSIEARSDGLGKGSEFIVRLPLLKQ
                     REDGKNSANVNDYASPMRRRILLVDDNQATTTVYAKLFETLGCEVAAANDGSKAIELA
                     ASFRPEIVMLDLSMPRMDGYETCRRIRQQPEGKNIVVLAVTGLGGNETVNRARLAGFD
                     DVIIKPVKADTILRLTHGVSDDDQTIS"
BASE COUNT          529 a          662 c          591 g          567 t
ORIGIN      
        1 aggtaaacgc aaaaaaggct gtggatcttc ccaacgtcgc caatgccatt gattatgcga
       61 agaccaacga agcacgccag ttgatcgatg cgggtgtcca tgcgacctcc gctatacttc
      121 gtgcctatgc tctgccgccg ggaacgccga aagaccgctt gaatttgctg cgcaagtcgt
      181 tcatggacac cttgaaggat cctgactttg tggcggagct aaaaaaagcc gatctcgacc
      241 tgaacccggt gacgggagcc gatgtcgagg ccaccgttca taaactgttt cagctcgact
      301 ccgggatggt aacgaaactg aaggagattt tggtaccgaa aaaatagccg aagtaaagct
      361 cacggatttt gtcgttgcct aacgccgttc aataaatcta ggaggtaaca tgccggacaa
      421 aatttccatt catccgaaga tcgactcggg cattcagccc gcggccaagg attttgcagg
      481 aggcgagctt gtctgccagt gcccgagcga acccgtgcgg gttaccgtaa acgctcaatc
      541 cgcgcacaac catgcgtgcg gatgcaccaa gtgttggaag ccgaaaggcg cgttattctc
      601 aatggtggcg gttgtcccac gcgacaagct gaaggtaacc gccaatgcga ataagctcgt
      661 cgtggtggat gctagtgcgg caattcaacg atacgcctgt acagaatgcg gcgttcatat
      721 gtacggccga atcgagaatg cgaagcaccc gttctacggg ctcgatttca tacacaccga
      781 gttgtcaccg caatccggct ggtcgccacc cgaattcgcc gccttcgtat cgtccatcat
      841 agagagcggc gtcgatccag ctcagatggg cgcagtgcgc gcacggctgc gagaactcgg
      901 gctcgagcct tacgactgtt tgtcgccggc cctgatggat ttcatcgcaa cgcatgtcgc
      961 gaaggcagcc ggagtcttgc ggtgatttct cagcgttcac ggcaggtgtt caacgtgaga
     1021 gcgtggctcc tgttatttca gctcgcctga aaaatagcag cacagtcgcg tttcacttcg
     1081 ttaccccgcg tacgcgcgct tcaatgtttc gatctcgatc tttttcatct tgagcatcgc
     1141 cgccatgacg cgctgggatt tcgtggcatc gggatcgttg agcatctcga ccaaagcccg
     1201 cggaatgatc tgccacgaca ggccgtattt gtctttaagc caaccacact gttgtgcctt
     1261 ttcgtctccg ccttccgaaa gtttttccca gtaataatcg acttcgtcct ggctcgtaca
     1321 atcaacctgg aacgagatcg cttcattgaa tttgaatacc ggtccgccgt tgagcgccgt
     1381 aaacacctgg ccgtccagtt cgaattcgac ggtcatgacc gagccctccg gcctgtgatg
     1441 aatttcgtac ccttccttcc catagcggct gattttcttg atcttggaat ttttaaagat
     1501 ttccgtataa aactccgccg cctgttccgc ttggtcatcg aaccataaac acggagtgat
     1561 cttttgcata agtggcatgg ctttcctcaa attggatcgg cggcgacgat caggaaattg
     1621 tctggtcatc gtcggatacc ccatgcgtca atcgcaagat agtatccgcc ttaacgggtt
     1681 ttataatgac atcatcaaag cccgctagcc gagcccgatt cacggtttcg tttccgccta
     1741 aacccgtcac agcgagaacc acgatgtttt tcccctctgg ttgctgccga atccgccgac
     1801 aggtctcata gccatccata cgaggcatac tcagatccag cataacgatt tccggtcgga
     1861 agctcgctgc aagttcgatc gctttgctgc cgtcgttggc tgcggcaact tcacaaccga
     1921 gagtttcaaa tagcttggca tatacggttg tcgtcgcttg attatcatcg actaataaaa
     1981 tccgccggcg catgggcgac gcataatcgt tgacgttcgc cgaattcttt ccatcctctc
     2041 tctgcttaag caacggcaag cggacgatga actcgctccc ttttcccagc ccgtcgctgc
     2101 gcgcttcgat gctcccgccg tgcagctcga tgagacgccg agccaacgta agtccaattc
     2161 ccaggccgct ggcgatggat tcgagcggcg gttgagcttg ataaaacaaa tcgaatagat
     2221 gatgcagact ttcttcggta ataccgatgc cattgtccac gatagaaaca agtgcattgt
     2281 cagcatcacg gctaaccctg atctgaatcc ttccgttctc cggcgtgtac ttagcggcgt
     2341 ttcccaata
//
LOCUS       DASULZ010000472         2552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_361425, whole genome shotgun sequence.
ACCESSION   DASULZ010000472 DASULZ010000000
VERSION     DASULZ010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361425"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="VFY96_09650"
     CDS             <1..1171
                     /locus_tag="VFY96_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5606768.1"
                     /translation="RLGAALEEAARVAGATTTAMLWRITVPLLSPMILAVSVLSFIRG
                     LQSFNTELLLGTPAGIYVYSTRIYDYIRREPASYGEATALGSVFIVALGILLFLYWRF
                     LRGKRRFTVVTGQGYSTARVRLGRWRYVASAGCTLYIAIMTLLPLAFLTIGSFMRRYG
                     FFSISSPFTLAHWQDVLSDPIFFLALKNSLLIATITAGVGVALYSIVAYVLVSRRAVM
                     APVLEGLCWLPHILPGILLGLGILWLFLATPLRFIFYGTIWGIALALMVSDSPVTTQA
                     FRAGYLQLGADLEEAARVAGASWGYTYRRILLPLLAPIAGAIGLMNFGGALTSISTPV
                     LLYSHPSRPLAILMLEYSVTGELERGAVLGLLITAMICVMMLIGRRLGLQLAGQQ"
     gene            1195..2286
                     /locus_tag="VFY96_09655"
     CDS             1195..2286
                     /locus_tag="VFY96_09655"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5606769.1"
                     /translation="MRHFGILVAALLWSASVLAQSTASETEWNTIVEAAKKEGKVVVA
                     GSPDPVMRNEIIPKFKERYGIPIEFIAGRSSEISARIKTERGSGIYSVDIYLSGPDTT
                     AATLYGEKMIDPLKPLLIMPEVADGTKWKLGKIWFADPEERFAVRAFSSVATLLFINT
                     EQVKPEEIRSAKDLLNPKWRGKISTEDPTTTGSGANSAARFYHDLGEDFVRKLYIEQK
                     TVRTRERRQMSDWLARGTQPICLSCREDDVRPLIKEGFKILEIFDLADMPGSINGSPW
                     MLTLANRAPNPKAAQLFANWILSKEGLGIYARGYGSVSMRTDIDETNLNPGNIPKKGV
                     KYFDDTDWKWIVTGRQEYREKVWQVLKGK"
BASE COUNT          541 a          660 c          753 g          598 t
ORIGIN      
        1 ccgactcggc gcggcgctcg aggaggcggc gcgcgtggcc ggcgcaacaa cgaccgcgat
       61 gctctggcgg ataaccgtcc cgctcttgtc gccgatgatt ctggcggttt ccgtgctgag
      121 tttcatacgc ggcttgcagt ctttcaatac ggagcttttg ctcggcacac cggcgggcat
      181 ctatgtctat tcgacacgca tttacgatta cattcgtcgc gagccggctt cttacggcga
      241 ggcgacagct ctgggttccg tttttatcgt tgcgcttggg atcctgctgt ttctgtattg
      301 gcgctttttg cgcggcaaac gcagattcac cgtggtgacc ggccaagggt attcgacggc
      361 gcgggtgaga ctcggtcgat ggcgttacgt ggcgtcagcc ggttgcactt tatacatcgc
      421 gattatgacg ctcttgccgc tggcttttct gaccatcggc tcgttcatgc ggcggtacgg
      481 attttttagt atcagctcgc cgtttacgct ggcacattgg caagacgttc tcagcgatcc
      541 gatttttttt ctggcactga aaaatagtct gctgattgcc acgatcaccg ctggcgtagg
      601 cgtggcgctc tattccattg ttgcgtatgt gctggtgtca cggcgggcgg tcatggctcc
      661 tgtcctcgaa gggctctgct ggttgccgca tattctgccg ggaattcttc tcggcctcgg
      721 gatcctgtgg ctatttctag ccacgccttt gcggttcata ttttatggca cgatctgggg
      781 catcgcgctg gccctgatgg tgagcgatag tccggttacg acgcaagcat tcagagcggg
      841 ttacctgcaa ttgggcgccg atctggaaga agcagcgcgg gtggccggcg cgtcctgggg
      901 ctacacctac cgaagaattt tactgccgct tctggcgccc atcgccggtg cgatcggttt
      961 gatgaatttc ggcggcgcct tgaccagcat cagcactccc gtgttacttt acagccaccc
     1021 atcgcgtcct ttggccattc taatgctcga atacagcgtg accggcgagc tcgaacgagg
     1081 tgccgttctt ggcttgctga tcacggcgat gatctgcgtg atgatgttaa tcggccggag
     1141 acttggattg cagttggcgg ggcagcaata gagactttgt cagtcggagg tggcatgcga
     1201 catttcggca ttctcgtcgc ggcgctcctg tggagtgcgt cggtgctcgc gcaaagcacg
     1261 gcgagcgaaa cggaatggaa cacgattgtc gaggcggcaa aaaaagaagg taaagttgtg
     1321 gtcgcgggct cgcccgaccc ggtaatgcgc aacgaaatta tcccgaaatt caaggaacgc
     1381 tacggcattc ccatcgagtt catcgccgga cggtcgagcg aaatcagcgc ccggataaaa
     1441 accgagcgcg ggtcggggat ttatagcgtt gatatttatc tttcaggccc ggatacgact
     1501 gctgcgacgc tctacggcga aaaaatgatc gatccgctca aaccgttact gatcatgcct
     1561 gaagtcgctg atgggacgaa gtggaaactc ggcaagatct ggttcgccga tcccgaggaa
     1621 cgcttcgccg tacgtgcgtt cagcagcgtc gcgacgctgc ttttcatcaa tacggagcag
     1681 gtaaagccgg aagaaatccg cagcgccaag gaccttctca atccgaaatg gcgcggcaaa
     1741 atttccaccg aagatccgac tacgacgggc tcgggcgcga actctgcagc gcgtttttat
     1801 cacgatctcg gcgaagattt cgtccgcaaa ctctacatcg agcaaaaaac tgtgcgcacg
     1861 cgcgaacgac gccaaatgtc agattggctg gcgcgcggga cacagccgat ttgcttgagt
     1921 tgccgcgagg acgatgttcg gccgctcatc aaagaaggct tcaagattct cgagatcttc
     1981 gaccttgccg atatgcctgg ctccatcaat gggtcgccat ggatgctgac attagccaat
     2041 cgggcgccga accctaaagc ggcgcaactg tttgccaact ggattctttc caaagaaggc
     2101 ttggggatct atgcgcgcgg ctatggctca gtgagcatgc gcacggatat cgacgagaca
     2161 aatctcaatc ctggaaacat cccgaaaaag ggcgtgaaat atttcgatga tacggattgg
     2221 aaatggatcg tcaccgggcg ccaggaatac cgggaaaaag tttggcaggt gttgaaaggg
     2281 aaataggttc aaacgttcaa gtcgttctag acgtttaaat cgtttcggaa ggcacgggtt
     2341 ctcaccctat taagagctgc gccgcgcgac caaggagttg aagcctagcg aatagaactt
     2401 ccaagaattg gacgttttga acaattggaa ctgtttgaac gttttgaact cttcgctcag
     2461 gggaaaagga aatttatggc caaaaaaatg cgcatctatt accgggcgcc gagtcatgtg
     2521 ccgttgtgga aggtgatgga ggcgggagga tt
//
LOCUS       DASULZ010000473         3049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1275536, whole genome shotgun sequence.
ACCESSION   DASULZ010000473 DASULZ010000000
VERSION     DASULZ010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275536"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VFY96_09660"
     CDS             <1..320
                     /locus_tag="VFY96_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606770.1"
                     /translation="ESYYEPVGPFFFYVETLTSTGPSNYGLTPTQPCVQSGVFKFGSV
                     SAAGSKYARAVSRCPVWSALRPACTCCPTINPEQPATLSADMRQINVEEVFGIIGAGL
                     QSG"
     gene            complement(329..1111)
                     /locus_tag="VFY96_09665"
     CDS             complement(329..1111)
                     /locus_tag="VFY96_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016879766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX5606771.1"
                     /translation="MSVFKNHWPEYLIEATCLAVFMISAFTFATILEHPASPIHRALP
                     DPLLRRFLMGLAMGSTAVGIIYSPWGKRSGAHINPSTTLTFFRLGKVEKWDALFYVAF
                     QFVGGLIGAISAAAALSAWASHPAVNYVVTMPGAAGALAALIAEVTIAFILMSVVLQV
                     SSTPRLHKFTGLCAGILVAIYITVEAPISGMSMNPARTFASAVPAHHWAYLWIYFVGP
                     VIGMTCAAEVYLRTRGATNTLCAKLHHQNTQRCIFCGKPAGV"
     gene            complement(1134..2246)
                     /locus_tag="VFY96_09670"
     CDS             complement(1134..2246)
                     /locus_tag="VFY96_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX5606772.1"
                     /translation="MRAIAVTPTEKKIGIIDLPEPAISSPTDVKLRMIEAGVCGTDKE
                     ICAFEYGTPPPGSSDLVIGHESLGEVIEVGAKVTQVKVGDLVIPMVRRPCPHDYCPAC
                     RSDRQDFCFTGDFTERGIKGRHGFMAQFVVDDERYMNPVPRDLRTVAVLVEPLTIAEK
                     ALIQVWDVQQRLPWNCPVTPGKAHAHCHRAIVLGAGPVGLLGAMALVKNGFETYVYAR
                     EPVPNPKADVIKNIGGHYFSAETHSLEALAAQVGDVDLVYEATGASRLAFEMIQHLGT
                     NGIFVFTGVPGRKGPVEVDTDLMMRNLVLKNQVVFGTVNAGRDAFEASIRDIGVFLDS
                     WPDAVQALITGRFPMEAHRDLLLGRSGGIKNVIRLN"
BASE COUNT          693 a          838 c          869 g          649 t
ORIGIN      
        1 cggaatctta ctatgagcct gtgggtccat ttttctttta cgtagagact ctgaccagca
       61 ccggcccgag caattacggt ttgacgccga cgcaaccgtg cgttcagagc ggggtcttca
      121 aattcggatc agtttcggcc gccggaagca aatacgcgag ggcagtttcc cggtgtccag
      181 tctggagcgc attgcggccg gcttgcacct gttgtccgac tataaatccg gaacagcccg
      241 cgaccttgag cgcggatatg agacaaatca acgtcgaaga ggtttttggc atcataggcg
      301 cgggactcca atcgggctga tttgctcgtt atacaccggc cggcttgcca caaaagatgc
      361 agcgctgcgt gttttgatgg tgaagcttcg cacagagcgt atttgtcgcg cctctggttc
      421 tgagataaac ctctgccgca caggtcatgc cgatcaccgg accaacaaag tagatccaga
      481 gataagccca atgatgcgcc gggacggcgg aagcgaaggt gcgcgccgga ttcatgctca
      541 tgccggaaat cggagcttcg acggtgatat agatcgcgac caagattccc gcgcacaagc
      601 cggtaaactt atgcagtctc ggggtactgg aaacctggag caccacgctc attaaaataa
      661 aagcgatagt cacctcggcg atcagcgccg ccaatgcgcc ggcggcgccc ggcattgtga
      721 cgacgtagtt cacggcggga tgtgaggccc acgccgagag agcggcggcg gcgctgatcg
      781 ccccgatcaa gccgccgaca aattgaaatg caacataaaa cagggcgtcc catttttcta
      841 ctttaccgag gcgaaagaac gtcagcgtcg tcgatgggtt gatatgggcg cccgagcgct
      901 taccccatgg agagtagatg atgccgacag ccgtgctccc catcgcgagc cccatcaaga
      961 aacgccgtaa gagtggatcg ggcaatgcgc gatgaatcgg ggacgccgga tgctcgagta
     1021 tcgttgcgaa cgtgaaggcc gagatcataa agaccgctag acatgtggct tcgatcaagt
     1081 attccggcca gtggtttttg aaaaccgaca tcgctctggc gtgtccgcat cgatcaattg
     1141 agtctaatta cattcttgat gccgccgctc ctgccgagca ggagatcgcg gtgtgcttcc
     1201 atgggaaagc gtccggtgat gagcgcctgg acagcatcgg gccagctatc gagaaaaacc
     1261 ccgatatcgc ggatcgaagc ttcgaaagcg tcgcgcccgg catttacggt tccgaacaca
     1321 acctgatttt tcaacaccaa gttgcgcatc atcaagtcgg tgtcgacttc caccggcccc
     1381 ttgcgtcccg gtactccggt gaacacaaaa atcccgtttg taccgagatg ttgaatcatc
     1441 tcgaaagcta aacgagaagc gccggtagct tcgtagacaa gatcgacgtc gccgacctga
     1501 gcggcgagcg cttcgagcga atgggtttcc gcagaaaaat aatgtccacc gatgtttttg
     1561 atgacatctg ctttcggatt gggcaccggc tcacgagcat agacgtaagt ctcgaagccg
     1621 ttttttacca atgccatcgc tcccagcaga cccacgggtc cggcgccaag aacaatcgcg
     1681 cggtgacaat gcgcgtgcgc tttccccgga gtgactgggc agttccatgg aaggcgctgc
     1741 tgcacgtccc aaacttgaat gagtgccttc tccgcgatcg ttaacggctc gaccaacaca
     1801 gcgacggtcc gcaggtcacg gggcacagga ttcatgtagc gctcgtcatc gacaacgaac
     1861 tgcgccataa agccatgccg tcccttgatc ccgcgctcgg tgaaatctcc cgtgaagcag
     1921 aagtcctgac ggtccgatcg acaagccgga cagtagtcat gggggcaggg ccgccgcacc
     1981 atcgggatga ctaaatcgcc cactttgact tgggtaacct ttgcgccgac ctcgatcacc
     2041 tcgccgaggg attcgtgccc gatcacgaga tcgctcgagc ctggcggcgg agtgccatac
     2101 tcgaaggcgc agatctcttt atcggtgccg caaacaccgg cttcgatcat gcgaagcttt
     2161 acgtctgtcg gggatgagat tgccggctcc ggcaaatcga taatgccgat ttttttctcg
     2221 gtgggagtaa ccgcaatagc tcgcataatg atctccttta cagtacagcg gctgggccga
     2281 cactcgtcga tgatgctgcc gtcttggatt agatgctgtt tgccgatcaa cgggtgcgct
     2341 gaagtgagtc cgggccactt ttatggcaag gcaggtttcc atccggccgg agcgcgagat
     2401 cctgccgatg cagaaagctt cgggtgagct cgatggtttt ccggtacgtg cgcagaattg
     2461 cagcacagtc cgtgcaccta ttaatgtggc ttccgaaagg gacggcagcc gaagcagtaa
     2521 tgcgcgccga tagataatca ggaatcaaat caactgcatt tcggcaggta atgaccatcg
     2581 gttctgtcct ggaggcgatt gcgttgtggg ctcgtggttt gttacgcgct atattaattg
     2641 tgtcgcagtt aaccgcgttt ttgttctcaa cgaaaacgga atggctccct aactgaccgc
     2701 tcggctcgcg ggcaaaaaat gcttaggctt ccggcctcaa ctacccggaa tttttagatg
     2761 ccttgtatga cagaaaagtt acgcgccgaa tcgtttgatt cgagaataca cgaacaggta
     2821 agcgcgcggg atccgcgata atacacggcg ctcaccgcga ccgggatccg ggaaagttgt
     2881 ccgggagcgg cagtatcgcg caggtcggag ctcctgcgcg atactaattg ctggcccgtc
     2941 caattcgaaa tggaaaacga aaacgtggga aacttacatc ggtctcagtt gacggcgagt
     3001 atgcggtttg ctctccggct ttgaattcca ggttcgctcc tgatcccga
//
LOCUS       DASULZ010000474         3215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1377983, whole genome shotgun sequence.
ACCESSION   DASULZ010000474 DASULZ010000000
VERSION     DASULZ010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377983"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VFY96_09675"
     CDS             <1..489
                     /locus_tag="VFY96_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606773.1"
                     /translation="RYGDQKLKANADAIMNHLDVFRPDCSLEELGRPEHVVDDFEAAG
                     IKSKDDVRGVFSNNFFFGCEADDRATMWAFDERMGVRLRPIFSSDFTHFDVPDFAEVI
                     PEAFEMVERGFVTEQDFREFTFVNAASLHTRNNPEFFKGTVVEKAVAEELGLSIAAAA
                     HA"
     gene            complement(589..1605)
                     /locus_tag="VFY96_09680"
     CDS             complement(589..1605)
                     /locus_tag="VFY96_09680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5606774.1"
                     /translation="MKLSVLDQSTASLGRTQDTAIRETLELARHCELLGYHRFWLSEH
                     HNSGSIVGTAPEILMAAIAATTSRIRVGSAGVMLPHYSALKVAEQFRVLEAIAPGRID
                     LGIGRAPGSDQMTAYALNPYPQNVLDQFPRQVQELQHWVSGKPLPEGHPFGNIIAQPT
                     GPTAPEMWILGSSEYGAQLAAYFGLPYAFAYFFSEGTGVEEALEVYRKNYRASEKYPE
                     PRATICVWALAADTEAEALHQFKTRERAIVDRRQGIRLPLMPPDEAERPYSAAELATA
                     EKLRRKAIVGTLDEVTPRLKDLATRLALDELVIVTWTYDPAPRRRSYELLSEAFGLRN
                     AKPC"
     gene            complement(1683..>3215)
                     /locus_tag="VFY96_09685"
     CDS             complement(1683..>3215)
                     /locus_tag="VFY96_09685"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX5606775.1"
                     /translation="LASIAAYFLVRFGLATNRWIMALILSTTSLGVVVPVLKESNLIG
                     ERLGQCILVSAVLADFATMLLITLAVAIVSRGLTAEILLIALLFVAFFLIYRFGTTFN
                     KVAALRRTIEELSHATAQIKVRAAFAIMLAFVALSQELGSEIILGAFLAGAIVGLLRT
                     ADDAELGHQLEAIGFGFFIPIFFVKVGIGFNLPALLASGGALLLVPFLFVGAVLVKMI
                     PALLFLVNFSLKESLAAGSLLSARLSLIIAAAAIGTRLGVIGESVNSAIILVAIGTVT
                     TAPLVFIRLLPGGQRTRRRNIVVVGAEDLGMQVARRLKAHLEPVCIVDADPERIGQAA
                     RRGFDTLSNSGEHDSAALRTLCQSARALVCTYDDADSAYRVCELAKNVYGVPHVVAQL
                     SDPSHRGRFDAIGVSTINAALDRPSLLALLARTPAIYTLFARTDDDKEVCEVILTNQH
                     DIDKPLRELELPAGLLALAIRRDGELLVPHGNTKLCSNDRLTLVGSIEDIEIAKQRFS
                     EP"
BASE COUNT          718 a          919 c          967 g          611 t
ORIGIN      
        1 cgctacggcg atcaaaagct caaagccaat gctgatgcga tcatgaacca tctggacgta
       61 ttccggccgg attgcagtct tgaagagctc gggcggcctg agcatgtcgt ggatgacttc
      121 gaagcggcgg gaatcaagtc aaaagatgac gttcgcggtg tgttctccaa taacttcttt
      181 ttcggctgcg aggcggacga tcgggccacc atgtgggcct tcgatgaacg catgggcgtg
      241 cggctgcgac ccattttcag ctccgacttt acacacttcg atgtccccga tttcgccgaa
      301 gtgattcccg aagcgttcga gatggtggag cggggcttcg tgaccgagca ggatttccgc
      361 gagttcacgt tcgtcaatgc ggcaagcttg cacacgcgca acaacccgga gttcttcaag
      421 ggcacggttg tggaaaaagc ggtcgcggaa gagctgggac tcagcattgc cgcggcagcc
      481 catgcctgaa cgaattggca gagccggcac aatgtggggc gaatgccatt cgcccctaca
      541 cccgtggtgc gggtctcctg tcctctgtga acagatatac cgacagcctc agcaaggctt
      601 cgcgtttcgc agcccgaaag cttccgacaa tagctcgtag gaacggcggc gcggtgccgg
      661 atcgtacgtc caggtgacaa tcaccagctc gtcgagggcc aagcgggttg cgagatcctt
      721 tagccgcggc gtcacttcgt ccagcgttcc gacaatcgcc ttgcgccgaa gcttctcggc
      781 ggtggccagt tccgccgcag aatagggccg ctcggcttcg tccggcggca tgagcggcag
      841 gcgaattcct tggcggcgat cgacgatcgc gcgttcacgg gttttgaact ggtgcagcgc
      901 ttctgcttca gtgtccgctg ccagcgccca gacacagatc gtcgccctcg gttcggggta
      961 tttttcactg gcacgatagt ttttccgata gacctcgagc gcctcttcta cgccagtgcc
     1021 ctcgctgaaa aaatatgcga acgcgtaggg caagccaaaa taggctgcca gttgcgcgcc
     1081 gtattccgag cttcccagaa tccacatctc cggagccgtt ggtccggtgg gctgagcgat
     1141 gatattcccg aagggatggc cttccggtaa tggcttgccg gatacccagt gttgcagctc
     1201 ctgcacctgg cgcgggaact ggtccagaac gttttgcggg taaggattga gcgcgtaagc
     1261 cgtcatctga tccgaaccag gcgcgcgccc aatacctaga tcgatccggc cgggagcgat
     1321 ggcttcgagc acgcgaaact gttcggcgac tttgagggct gaatagtgcg gcaacatgac
     1381 tccggcgctg ccgacgcgga ttcgcgacgt cgtcgccgcg atggcggcca tcagtatctc
     1441 gggcgctgtg ccgacgatgc tgccggagtt gtgatgctcc gagagccaga aacgatgata
     1501 acccagcaac tcgcaatgac gggcgagctc aagagtttcg cgaatcgctg tgtcctgggt
     1561 tcttcccaag gatgccgtcg attgatcaag gactgacagt ttcatagcgg atctgttttc
     1621 ccggatttct atcaccggag cggcacagcg taaactcgcg cggcgcgctt cggtttgcgc
     1681 ttttacggct cggaaaatct ttgcttagca atttcgatat cttcgatgga gcccaccaag
     1741 gtcaagcggt cgttggagca gagcttggta ttgccgtggg ggaccagcaa ttcgccatcg
     1801 cgccgaatcg ccagggccag gagaccagcg ggaagctcca gttctcgcag cggtttgtcg
     1861 atgtcgtgct gattggtgag gatgacttcg cagacttctt tgtcgtcgtc agtgcgcgcg
     1921 aacaaggtat agatcgcggg cgtgcgtgcg agcaaagcca acagagaggg acgatcgaga
     1981 gcagcgttta tggtcgatac gccaatggca tcgaaacggc cccgatggct cggatcgctc
     2041 agctgcgcga ccacgtgcgg gactccataa acgtttttcg ccagctcgca aacccgataa
     2101 gccgagtcgg catcgtcgta agtacacacc aaggcgcgcg ccgattggca aagcgttctc
     2161 agcgctgcgc tatcatgctc acctgagtta gacagagtgt cgaagcctcg gcgcgccgct
     2221 tgcccgatgc gctcggggtc cgcgtcgaca atgcaaaccg gttcaagatg ggcttttagc
     2281 cgcctggcaa cttgcattcc caaatcttca gcgccgacca ccacgatatt cctgcgccga
     2341 gtgcgctggc cgccggggag caaacgaatg aacaccaatg gggctgtcgt gacggtgccg
     2401 atggcgacta aaatgatcgc agaattcacc gactcgccga tcacgccgag ccgggtgccg
     2461 atggctgcgg cggcgatgat cagcgacaac cgcgctgaga gcaacgaccc cgcggccagg
     2521 gactctttca gtgaaaaatt caccaagaac aggagcgccg gaatcatctt taccaaaacc
     2581 gcgccgacga acagaaacgg aaccaaaagc aaagcgccgc cggacgcgag cagcgccggc
     2641 agattgaatc caataccgac tttaacgaaa aaaatcggaa tgaaaaaacc aaaaccgata
     2701 gcttcgagtt gatgtccgag ctccgcatcg tccgcggtgc gcagaaggcc gacgatcgcg
     2761 ccggcgagaa aggcaccgag aataatttcc gagccgagtt cctgggaaag cgccacgaag
     2821 gcgagcatga tcgcgaaagc ggcgcgcact ttaatttgcg cggtggcatg gctcaactcc
     2881 tcgatcgtgc ggcgcagcgc cgccactttg ttgaaggtcg tgccgaatcg gtaaatcaaa
     2941 aaaaaggcga cgaacaacag agcgatcaat aaaatctctg cggtcaagcc gcgcgaaaca
     3001 atggcgaccg ccaaggtgat caggagcatg gtggcaaaat cggcgagcac ggcgctcacc
     3061 agaatacatt gaccgagccg ctcaccaatg agattggatt ctttcagaac cggcacgacc
     3121 acgccgagcg acgtcgtcga caaaatgagc gccatgatcc aacgatttgt cgccaggccg
     3181 aagcggacga ggaagtaagc ggcaatggag gccaa
//
LOCUS       DASULZ010000475         2991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_260736, whole genome shotgun sequence.
ACCESSION   DASULZ010000475 DASULZ010000000
VERSION     DASULZ010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260736"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFY96_09690"
     CDS             <1..345
                     /locus_tag="VFY96_09690"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5606776.1"
                     /translation="AEFDVGWVANLVQQVDYWFGRASTYDAEKNINKQPPSAYFKSNA
                     FFTYQDDRAGVLTTPVFGADNYLWASDYPHGVTTWPNSQATVERNCAGIDPAVKRKLN
                     RDNVAKLYELSK"
     gene            424..642
                     /locus_tag="VFY96_09695"
     CDS             424..642
                     /locus_tag="VFY96_09695"
                     /inference="COORDINATES: protein motif:HMM:NF018595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX5606777.1"
                     /translation="MKITVDRTKCEGYAKCVQASPKVFRLDAKMIAEVVDPKADADEK
                     IVLAAKICPTKAISLEEQGTGKKIFPFD"
     gene            complement(712..1434)
                     /locus_tag="VFY96_09700"
     CDS             complement(712..1434)
                     /locus_tag="VFY96_09700"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606778.1"
                     /translation="MVYDCWRGMVGIIKPTKGSGSLVELIKLLPDGIGVIPLFNDVRH
                     GKIEEFRGAIPAYEEKIAELAQDQVDLIHPAGTPPFMLLGYNGEAELIAKWEKRFNIP
                     IFTSGTNQIAAMKALGIKKFVGIGYDFDDTSIVARYFTDAGFRVLSLEKLPGPWEEVG
                     RLSSKDIYDQAKNLFLRHSEADGIYFQGGKLRILDIIEPLEQDLGIPVIHPGLAQCWE
                     IQKRLHVRQPIAGYGRLIAELP"
     gene            complement(1630..1779)
                     /locus_tag="VFY96_09705"
                     /pseudo
     CDS             complement(1630..1779)
                     /locus_tag="VFY96_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011452885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
     gene            complement(2080..2829)
                     /locus_tag="VFY96_09710"
     CDS             complement(2080..2829)
                     /locus_tag="VFY96_09710"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5606779.1"
                     /translation="MMRLKDKVAVITGGAQGIGRAICLSMAREGANVVVADLQGDKAK
                     SVVAELQAIGAEAIYAEVNVANEMSVKRLADQTFERFGRIDILVNDAGVYLKAPVVNK
                     SEADWDHTINVNLGGNFLCARAFMPAMRQQKSGRIISLASSIAHTGAREFADYAASKA
                     AIIGFVKALARELGPEGITVNAICPGSANTDMPRQHRTEEETMARLRSTPLEHVLEPD
                     DIAGPIIFLASDAAAYITGQVYNINCGTVMV"
BASE COUNT          677 a          850 c          769 g          695 t
ORIGIN      
        1 gccgagttcg atgtcggctg ggtcgccaat ctggtgcagc aagtcgatta ttggttcggc
       61 cgcgccagca cctacgacgc cgaaaagaac attaacaagc aaccgccgag cgcatacttc
      121 aagagcaatg cattctttac ttaccaagat gaccgcgccg gcgtgctgac aactcccgtc
      181 ttcggcgcag ataactatct ctgggcaagc gattatccgc acggcgtgac gacctggccc
      241 aactcgcaag cgaccgtcga gcgcaactgc gcagggattg atcccgccgt gaagcggaaa
      301 cttaatcgcg acaatgtggc caagctatat gagctaagca aatagaggtg tctcattgag
      361 ccgtcgaagg ttggattcga acccatcact ccaatactcc acactccaga aattgaggag
      421 actatgaaaa tcaccgttga tcgcaccaag tgcgaaggct acgcgaaatg cgttcaggca
      481 tcgcccaaag tgttcaggct cgacgcgaaa atgattgcgg aagtggtcga tcccaaggcc
      541 gacgccgacg aaaagatcgt gctcgccgct aaaatttgtc cgacgaaagc catcagtctc
      601 gaagagcaag gcaccgggaa aaaaattttt ccgttcgact gagtgaaatc gtcggctgcg
      661 agcgctctcc tacagccaga ctgtttgtct ctgtgtgcct agcgaagtga gttatggcaa
      721 ttcggcgatt aatcgcccgt atcctgcgat cggctgtcgc acatgcagcc gcttttggat
      781 ttcccaacat tgcgccagtc cgggatgaat cactgggata ccgaggtcct gctcgagcgg
      841 ctcgatgata tcgaggatgc gcagcttgcc gccctgaaaa taaattccgt ccgcctcgga
      901 atggcgcaaa aacagatttt tcgcttggtc atagatatcc ttcgatgaga gtcgtccgac
      961 ttcttcccaa ggcccgggca gcttttccaa tgataaaact ctgaacccgg catccgtgaa
     1021 gtagcgcgcc acgatgctgg tatcgtcaaa gtcgtagccg ataccaacaa atttctttat
     1081 ccccagcgcc ttcatggcgg caatttggtt ggttcccgaa gtgaagatcg gaatgttgaa
     1141 gcgcttttcc cacttcgcaa tcaactcggc ctcgccattg taaccgagca acatgaaagg
     1201 cggcgttccc gcgggatgaa tcagatcgac ttggtcttgc gccaattccg cgattttttc
     1261 ttcgtaagcc ggaattgccc cgcgaaactc ttcgatcttg ccatggcgga cgtcgttaaa
     1321 gagcgggatt acgccaatcc catcgggcaa cagcttgatc aactcgacca atgagcccga
     1381 tcctttggtc ggtttgatga ttcccaccat accgcgccag cagtcgtaga ccatcgtcgt
     1441 ttgtcctttc gattgacgga agatctattt ctcattgtgg cggtctcgca cgacctgcag
     1501 ctcttccaaa caccccgggc acatataagc ggcatggggc aaaaaaatca gttcgtccgg
     1561 ttcgcgcgcc tccatacaga ttccgcaaag ccgctcttgc ttgcgcagcg agacggcatt
     1621 tttgttttcc gactcctccg cgataatgcc gctacagagc cccacacact cattgcaaat
     1681 ctgtaccgca ggaccggcga tcaggcgccg cacctcgtgc tctccttttg cgcagaagga
     1741 acagctggat tcggattgct catcgctgaa ttgcggaatt tcctgttccc agcggtcgcc
     1801 gtaaggtttc ttaagagtgt cttccaaaac agtaccgatc tgaatgagtt gtcgcgcaat
     1861 ttcagttcgt ttcggcgtgc ttttcgattc aagaaaacga ctgaccgcga gctgaaaaaa
     1921 tttcagagcg cgggcatagt gttgctccgt gatgagatcg gcgcattcta caaaaccaaa
     1981 gtttgacgaa ctctttgcca tcgatggtct cctagatcag ttgcctaaaa gcagcgcagc
     2041 gttgggccga agcgtgacac tttaagaatg gttaataaat cagaccatca cggtgccgca
     2101 attgatgttg tagacctgcc cggtgatata ggcggcagca tcgctggcga gaaaaataat
     2161 cggcccggcg atatcgtcgg gctcgagcac gtgctctaaa ggcgtagagc gcaaacgcgc
     2221 catcgtctct tcctcggtcc ggtgctggcg cggcatatcg gtattcgccg agccgggaca
     2281 gattgcgttg acagtaatgc cctccggccc gagttcacgc gccagcgcct tgacgaaacc
     2341 gatgatggcc gctttggacg ccgcatagtc ggcaaattct cgtgcgccgg tatgggcaat
     2401 ggaggatgca agactgatga ttctgccgct cttctgttgg cgcatagccg gcatgaatgc
     2461 gcgggcgcaa agaaaattgc cacctaagtt gacgttgatc gtgtgatccc agtctgcttc
     2521 gcttttgttc accaccggcg ccttcaagta aacccccgca tcattgacca gaatgtcgat
     2581 gcggccaaaa cgctcgaagg tttgatcggc gagccgtttc actgacatct cgtttgcaac
     2641 attcacctcg gcgtaaatcg cctcagctcc gatcgcctgg agctcggcca ccacactttt
     2701 ggctttgtcg ccctgtaaat ctgcgaccac gacgttcgcg ccttcccggg ccatgctcag
     2761 gcaaatggcg cggccgatgc cctgcgcgcc accggtgatc accgcaacct tatcttttaa
     2821 tcgcatcatt cgtttgcgcg tccctcagcg aattctgccg gaatgatgag ggcgatcgcc
     2881 gatcgccccg acaattctcc gtttccttcg tgttctttgt gggttaattg tttttacctg
     2941 ctttagatca agccaccgtt acagtgaaac aactgcccgg tgatcgctgc g
//
LOCUS       DASULZ010000476         3625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_971582, whole genome shotgun sequence.
ACCESSION   DASULZ010000476 DASULZ010000000
VERSION     DASULZ010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971582"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..1703)
                     /locus_tag="VFY96_09715"
     CDS             complement(21..1703)
                     /locus_tag="VFY96_09715"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5606780.1"
                     /translation="MARITGSDLICKSLKLEGVKNIFGLAGDHILPLLDVMSDQDFRI
                     IDTRHEQAAVHMADAWSRITEQPGVCMYTTPGFANAIPGLTNAMHTEGAVISIAGCAD
                     THDLGRGAQQEIEQVAMAASVCKGSFMVPDARRIPEFIARAIRLAFSGRRGPVHLTIP
                     IDVQEQSVDEEEIVLTRPEEYRPKESVLTSDELVRQAIALLRQAQRPLAIAGCAAGYT
                     LSGEALQHFIETTRVPVVTEEQARGLVSDNHPYVFGFFERGLNRAAAKVRDADVVVLL
                     GRKQDFVIGFCRPPHVSADAKIIQIDPSPLEIGRNRGVAVGIVGDVTSVLEQLTKAAS
                     NHTWKELPWIQELRAVRAAQAEWAEKLAQPKTPMHALFVHKTIQSILRPDDCLVFDGG
                     DFCHFGRSLLPALEPKHWLYVSSLGMLGSSLPQALAAKVAYPNSRVIMLTGDGAFGFN
                     AMEFDTAVRHKLNVVAILGNDSAWGIDRQIQLGLYGRPVATDLLQTRYEQVVQGLGGY
                     GEFVERSEDFAPALQRALDSGKPALLNVAVERAISPRAEAAIGRRKAAVPKN"
     gene            complement(1761..3071)
                     /locus_tag="VFY96_09720"
     CDS             complement(1761..3071)
                     /locus_tag="VFY96_09720"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5606781.1"
                     /translation="MISRAALGFLPNISSRIFYGWRMVGLVSLIRVVGGGLHQFGFTV
                     FFLPISQDLGLSRAATSLAFSLSRAQGAIEAPLVGHLIDRYGPRPIIVTAVFLAGVGY
                     ILLSWVNSYASFLTVYLGVICLAFVAGFVHSPLVVANNWFIRQRARAMTVISAAVPVG
                     GALISPLLAVGVASIGWRWAAFASGCIFLTVCLPLSFQLKRSPESLGMLPDGEDPLDS
                     ADVTSSGKIANVTAAADVTSRQAMKTWIFWLLVIAMTARVTCYSTATVHFVPLMVWKG
                     LSEAAAASLLGAFAFINLVAHFVLGWIADRVNKPKLLAACHLLPALSLPALLAGSDYW
                     QLLLFTTTFTFLDASFPIVWATVGDFFGRRNFATIRGMMSFFYMWGSFAGPIMAGAIY
                     DRTQSYFMVLWILLALLVFATFLVLFLIRPWTKRMIALVPVGCS"
BASE COUNT          853 a         1030 c         1035 g          707 t
ORIGIN      
        1 cggtgcctcc gtggtagtaa ctagtttttc ggcacagcgg ctttgcgccg gccgatggcg
       61 gcttcggcgc gcgggctgat ggcgcgttcg acagcgacgt tcagcaatgc gggctttcca
      121 gaatccagcg cacgctgcaa tgcgggcgcg aaatcttcag atcgctcgac gaattcgccg
      181 tagccgccca atccttgaac gacctgctcg tatcgtgtct gcaacaaatc ggtggcgacc
      241 ggccggccgt aaagtcctaa ctgaatttgc cgatcgattc cccaggcgga atcattgccc
      301 agtatggcaa cgacattcag cttatggcgc accgcggtgt cgaactccat cgcattgaat
      361 ccgaatgcgc cgtcgccggt gagcatgatc acgcgagagt tgggataagc gactttagcc
      421 gcgagcgcct gcggcagtga cgaaccgagc atgcccaagc tcgagacgta gagccagtgc
      481 ttgggctcga gcgccggcaa caacgaccgc ccgaaatgac agaaatcacc gccgtcgaac
      541 acgagacaat catcagggcg caggatcgat tgaatcgttt tatgcacgaa cagcgcatgc
      601 atcggcgtct tgggctgcgc cagtttttcc gcccattccg cttgggcggc acgcaccgcg
      661 cgcaattcct ggatccacgg cagctctttc caggtatgat ttgaagctgc tttggtcagt
      721 tgttccaaga cgctggtgac gtcacccacg atgccgacgg cgacgcctcg gttgcggccg
      781 atctccaatg gcgatggatc gatctgaatg attttggcat ctgcggaaac gtgaggtggc
      841 cggcaaaagc cgatcacgaa atcctgcttg cgccctaaga gcacgaccac gtcggcatcg
      901 cgtacttttg ccgcagcgcg attcaagccg cgctcgaaaa atccgaaaac gtacggatga
      961 ttgtcggaaa cgagtccgcg tgcttgttct tcggtaacga ccggcacgcg agtagtttcg
     1021 ataaagtgct gcaaagcctc gccggaaagg gtataacccg cggcgcagcc ggcgatcgcc
     1081 agcggcctct gcgcttggcg caggagcgcg atagcttggc ggacaagctc gtcgctcgtc
     1141 aacactgatt ctttagggcg atattcttcc ggtcgggtta agacgatttc ctcctcgtcg
     1201 acgctttgct cctgcacatc gatcggaatc gttaaatgca cgggaccgcg ccgtccgctg
     1261 aacgcgagac gaatggcacg tgcgatgaac tccggaattc tgcgcgcatc cggcaccatg
     1321 aacgagcctt tgcacactga cgcggccatc gcgacctgct cgatttcctg ttgggcgccg
     1381 cgtcctaggt cgtgcgtatc ggcgcagccg gcaatggaga tgaccgcgcc ttctgtgtgc
     1441 atggcgttag tcagacctgg aatcgcgttg gcgaaaccgg gcgtcgtgta catgcagact
     1501 cctggctgtt cggtgatccg gctccaggca tccgccatat ggaccgcggc ttgctcgtgg
     1561 cgcgtatcga tgatccgaaa gtcctgatcc gacatcacat cgagcagcgg cagtatatga
     1621 tcacccgcca agccgaaaat attttttacg ccttcgagct tgagagattt gcagatcaag
     1681 tcacttccgg tgattcgcgc catggatcgt ctcccgatta gtcagattgt gctccctata
     1741 acatcgccta cacttttgtt ctacgaacag ccaaccggca cgagtgctat catgcgtttt
     1801 gtccaagggc ggatgaggaa caaaaccaaa aaagtggcga acaccagcag cgcgagcagg
     1861 atccaaagga ccataaaata actttgcgtg cgatcgtaaa tcgcgcccgc catgatcggg
     1921 cccgcaaagc ttccccacat atagaagaaa ctcatcatgc cgcggatcgt cgcgaaattg
     1981 cgccgtccaa aaaaatctcc cacggtcgcc cagacgatcg gaaacgaggc gtccaaaaaa
     2041 gtgaacgtcg tggtaaaaag cagaagctgc cagtaatccg agccggccaa gagcgccggt
     2101 agagaaagcg ccggcagcag atgacaggct gccaagagtt tcggcttgtt cactcgatcg
     2161 gcgatccaac cgagcacgaa atgcgcgacc aaattgataa atgcaaaggc gccgagaagc
     2221 gatgcagcgg cggcttccga caatcccttc caaaccatga gtggcacgaa gtgcacggtg
     2281 gcggtgctgt aacaggtgac gcgcgccgtc atcgcaatta ccagcagcca gaaaatccag
     2341 gttttcatcg cctggcggct ggtgacgtcc gctgccgcgg ttacattggc gatcttaccg
     2401 gaagaagtta catccgccga gtccagcggg tcttccccgt caggaagcat gcccaaactt
     2461 tccggcgatc gctttagctg gaagcttagc ggcaaacaca cggttaaaaa aatgcagccg
     2521 gaggcaaatg ccgcccaacg ccagccgatc gacgccacgc cgactgccaa aagcggcgag
     2581 atcagcgcac cgccgaccgg caccgccgcg ctgatgacgg tcatcgctcg cgcccgctgg
     2641 cgaatgaacc aattgttggc caccaccaat ggcgaatgaa cgaagccggc gacgaaggca
     2701 aggcaaatca cgccgagata aacggtgaga aagctcgcgt agctatttac ccacgacagt
     2761 agaatgtaac cgacgccggc cagaaaaacc gccgtcacga tgatcggtcg cggcccgtag
     2821 cgatcgatga gatgtccgac cagcggcgcc tcaatggcgc cttgcgcgcg tgacagagag
     2881 aaagccaaag acgtcgcggc gcggctgagt ccaagatcct ggcttatcgg tagaaagaaa
     2941 accgtgaagc cgaattgatg caacccgccg ccgaccacgc ggatgagaga aaccaatccc
     3001 accatgcgcc agccgtaaaa aatgcgcgag gaaatgttgg gtaggaaacc caaggcggcg
     3061 cgagaaatca cgcgccgacg ttatgtgaat gaaagggccg gcgtcaaggg aatgaaggaa
     3121 aagaaccggt ctcaacttga tagcggttca attcatctca aacagaattc cggtttttct
     3181 cgaaatgtcc agcaaatcgt ccaccacggg cttgaccagt ggaatgcctt ctttgcttcg
     3241 aatcgcttcg gcttcgcgct ccgaatcgcc ggggatcagc gggccgtgga tgccgggcgc
     3301 cggcttggta ttgcggaaga cgtgaatcca ttcgtcgatt gtttctggaa ctcgtctttg
     3361 tcgataaaac catcgatctg ctgggcgccg aagaaatggc cgagcgggag atgccgtctg
     3421 agcctatatg ctattgtggc taaattgctt gaaaggcaat ttgtgctcaa tcgggtacag
     3481 aataaaaagc ttctggcgtt gcatcctatt cccttgaaaa tcccttggta gggggtttcg
     3541 ctagaataca atgcaacaag ctggaagtcc ggatacggga gcgtgagctt taggttcgtt
     3601 attgctataa tccggtcgtt ggatc
//
LOCUS       DASULZ010000477         3145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_260765, whole genome shotgun sequence.
ACCESSION   DASULZ010000477 DASULZ010000000
VERSION     DASULZ010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260765"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1548
                     /locus_tag="VFY96_09725"
     CDS             <1..1548
                     /locus_tag="VFY96_09725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5606782.1"
                     /translation="EALLAGPGRKIVTVSSAQDALRQLLRQEQFSLILLDVRMPDMDG
                     FEAADLIRQSERLRHIPIIFLSAIDTLEADVYRGATKGAVDYLFKPVVPEVLKAKVSV
                     FVDLFHINERLKLKAIQQSEERFRLLVESMEDYAIYMLDPEGNVTSWNTGAERIQGYR
                     QQEIIGERFNRFYTPEDQEQDLPARALSYAVANGRSEHEGWRVRKDGARFWANTIVSA
                     LLDERHSLVGFSVVMRDLTDRREAEEALRESERKLRRQAEELEQQLIASGRLVSLGEI
                     TASMAHEFNNPLGIVMGFAQDLMSEIDPTGPHYRYLKIIDEETKRCQKIIQELLQFSR
                     PSAAELRPTEIEKLVEKTAHLVENRLYQQKIELSLDVDRELPLVQADAQQLEQVLINV
                     YLNAVDAMPHGGKLTVGAKLSPPNNGDQPLVILSVADTGFGIEEENHGKIFLPFFTAK
                     KTKGLGLGLPICERIIKNHGGRIEFSSVPGEGTTFRIYLPLSRPPDLGNALMSRTEMP
                     PESEPAS"
     gene            complement(1718..2896)
                     /locus_tag="VFY96_09730"
     CDS             complement(1718..2896)
                     /locus_tag="VFY96_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia lyase-activating protein"
                     /protein_id="HEX5606783.1"
                     /translation="MVMLGTDMKVAETPKEGLKDYAKADPYTEWLKKEGVKVYQDFYF
                     PSLAKVELGPWERKGGSGAVIHIDNRHMPNDCHVVEIKPGGKSEPEHHLYELTIYVVS
                     GRGATTIWQDPPNGSGGKGKNSFEWQAGSLFSIPLNAWYQNFNGSGNEPARYIAVTNA
                     PPMMRLFRDTDFIFNCDHMFRGRYSGEEDYFSGKGKLYTRRIWESNFIANAPDMLLYG
                     WKERGAGGINAMLEMADNNTKSHISEFPVGTYKKAHRHGPGAHLVLLSGTGGYSLIWT
                     KEDRSDMIKCDWQVGSMVTVPSDNCYHQHFNSGTTRARYLALRPGDMGLYKGRGGGGE
                     NADRSMKEGGWQIEYEDEDHEIHKVFENELKAHGAVCRMKAFVPWCTGEVGPTSERDT
                     "
     gene            complement(2960..>3145)
                     /locus_tag="VFY96_09735"
     CDS             complement(2960..>3145)
                     /locus_tag="VFY96_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606784.1"
                     /translation="KYYCSPRYRASDQAKNNSVQRVPSWSAAETSLKPLQQSLSAILI
                     IEAILVLTSVDHQLEAR"
BASE COUNT          704 a          880 c          828 g          733 t
ORIGIN      
        1 gaagcgctgc tggccgggcc cgggcggaag attgtcaccg tctcgagcgc tcaggacgcg
       61 ctgcggcagt tgctacgcca ggaacaattt tcactgattc tgctcgatgt tcgcatgccc
      121 gacatggacg gctttgaagc cgccgatctc atccggcaaa gcgagcgcct gcgtcatatc
      181 ccgatcattt tcctctccgc catcgatacg ctggaggcag acgtgtatcg gggcgcgacc
      241 aagggagcgg tcgattatct cttcaaaccg gtggttccgg aggtcttgaa agcgaaagtt
      301 tctgtgttcg tcgacttgtt tcacatcaat gagcgcctga agctcaaggc cattcagcaa
      361 agcgaagaac ggtttcgact tctggtcgag agcatggaag attacgcgat ctacatgctt
      421 gatccggaag gcaatgtgac ttcatggaat accggcgctg agagaattca aggatatcgg
      481 cagcaagaaa tcattggtga acgtttcaat cgcttttata ctcctgaaga ccaggaacaa
      541 gatctgccgg cccgggcgct cagctatgcc gtagccaacg gtcgaagcga gcacgagggc
      601 tggcgcgtgc gcaaagacgg cgcacggttt tgggccaaca cgatcgtctc ggcattgctc
      661 gacgagcggc atagtctagt tggtttttcc gtcgtgatgc gtgatttgac cgatcggcgc
      721 gaagcggagg aagcgcttcg cgaaagcgaa agaaagttgc gccgccaagc cgaagagctc
      781 gaacagcagt taatcgcctc agggcggctg gtgtcgcttg gcgaaatcac cgcttccatg
      841 gcgcacgaat tcaataaccc gcttggaatc gtcatgggtt tcgcacagga cctcatgagc
      901 gagatcgatc cgaccggtcc gcactatcgt tatctcaaga tcatcgatga ggaaaccaag
      961 cgctgtcaga aaattattca ggagcttcta caattttcac gaccaagcgc cgccgagctc
     1021 cgcccgaccg aaatcgaaaa actggtagag aaaaccgccc atctggtgga aaaccgtctc
     1081 taccaacaaa aaatcgagct ttccttggat gtcgatcgcg aattgccgct ggtccaggcc
     1141 gatgcgcagc aattggaaca ggtattgatc aatgtttatt tgaatgccgt cgacgccatg
     1201 ccgcacggcg gcaaattgac cgtcggtgca aaattgtcgc cgcccaacaa cggcgaccaa
     1261 cctctcgtaa tcctaagtgt tgctgatacc ggattcggca tcgaggaaga aaatcacgga
     1321 aaaatcttct tgccgttttt tacggcgaaa aaaaccaaag gcttgggact gggactaccc
     1381 atctgcgaac gaattatcaa aaatcacggc ggacgaatcg agttcagcag tgtgcccggt
     1441 gagggcacaa cgtttcggat ttatctgcca ctcagcagac cacccgacct gggcaatgcg
     1501 ctaatgtctc ggaccgaaat gcctcccgaa tctgagccag caagctaacc gccggccgcg
     1561 gcacccgacg cgctctcgcg atcaaacagt atcgttatcc cgagcggcag actctggccg
     1621 ttcgcccttc gatacggcag cgctgctcgc tgcctactca ggatgctcga aacggtccga
     1681 cttcccgagt agcccgaagc gcgtatcgag ggatcatcta cgtatcacgc tcgctcgtcg
     1741 ggccgacctc acccgtacac caggggacaa atgccttcat tcgacagacc gctccgtgcg
     1801 ccttgagttc attctcgaag actttgtgga tttcgtggtc ttcatcttcg tattcgatct
     1861 gccaaccccc ttctttcatg cttcggtcag cgttttcgcc gccgccaccg cgtcctttgt
     1921 aaagacccat atcgcccggt ctcagcgcca aatagcgcgc ccgcgtggtt cccgagttga
     1981 agtgctggtg ataacaatta tcactcggca cagtgaccat agaaccgacc tgccagtcgc
     2041 atttgatcat gtcggaccgg tcttccttgg tccaaatcag cgaatagccg ccagtgccgc
     2101 tgagcaaaac caggtgtgct cccgggccgt gacggtgcgc tttcttgtag gtcccgacag
     2161 ggaattcgga aatgtggctt ttggtattgt tgtcggccat ttcgagcatt gcgttgatgc
     2221 ccccggcgcc gcgctctttc cacccgtaga gcagcatgtc aggtgcattg gcgataaagt
     2281 tagattccca gattcttcgc gtatagagtt tacctttgcc gctgaaataa tcttcctcgc
     2341 ctgagtaacg accgcgaaac atgtgatcgc aattaaatat gaaatcagtg tcacggaaca
     2401 agcgcatcat tggcggcgca ttggtgacgg cgatataacg cgcgggctca ttgccgctgc
     2461 cgttaaagtt ttgataccag gcgttgagcg gaatcgagaa caagcttccg gcttgccact
     2521 cgaagctgtt cttacctttg ccgccagagc cgttgggcgg gtcctgccag attgtcgtcg
     2581 caccccgtcc cgacacgaca taaatcgtca gttcatataa atgatgctcg ggctcggatt
     2641 tgccccccgg tttgatctcg accacgtgac agtcgttggg catgtgccga ttgtcgatgt
     2701 gaatcaccgc gccgctgccg ccctttcgct cccacggacc cagctccacc ttcgccaagc
     2761 tgggaaaata aaagtcttga tagactttga cgccttcttt cttgagccat tctgtataag
     2821 gatccgcttt ggcgtaatcc ttcaaacctt cctttggcgt ttcggcgact ttcatatctg
     2881 tgcctagcat caccacggtt cgttcctcct tgagcgaaca tgctcttagt gtttggatat
     2941 ccggtatgag cccaacttgt caacgagctt cgagttggtg atcaacactt gttaacacca
     3001 gaatagcttc tataatcaaa atagcggaaa ggctttgctg aagcggtttg agggaagtct
     3061 cggcagcact ccatgatggc acgcgttgca cactgttgtt tttggcttgg tctgacgctc
     3121 tgtaccgtgg gctgcagtaa tactt
//
LOCUS       DASULZ010000478        15339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_870525, whole genome shotgun sequence.
ACCESSION   DASULZ010000478 DASULZ010000000
VERSION     DASULZ010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 15339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15339
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870525"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VFY96_09740"
     CDS             <1..350
                     /locus_tag="VFY96_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606785.1"
                     /translation="EGGGTGSTFKVGSASNFLGLSPLAAVRNRFYDIHAFANKGSSLL
                     HVIDQPKCEIECEQFYDCGNRRFGPEFVITYSMTRDSIPREGGGFNAVTRVTVSKAAK
                     SAAASPSGSGSTP"
     gene            460..2097
                     /locus_tag="VFY96_09745"
     CDS             460..2097
                     /locus_tag="VFY96_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX5606786.1"
                     /translation="MNQTAALQTRTEKSPPSSRHSQLVQRKCGCGGSSGVTGSCSECD
                     KKKLLGQSFQARLRINEPGDAYEQEADQVAARVMQMTERDLRLGLRQSGGLVQRRITG
                     HELSGHLVPTLQRQAAASEPTPAEGSPQSSDTPSQKEEEESSCPSWRGDPESISKRAA
                     ETYVRNDMTPPSQATVERIDCEPPVPNGNYGCYVHFSDGLVIRVIVRAKDIVVGTGPG
                     PFTTLTPPPATPLCFYDYTCPEGQLILSKRECKSAKPAGSSGPTLVGQRRAAAGASGM
                     LDAGPFVDAVLASPGKPLDAAAEAFFSARFGHDFRNVRVHADAAAAESARAVNALAFT
                     VGNHVVLGAGQYSPRTRAGQELLAHELTHVVQQGAAAPRAAEIHDQGFSGTPAVSSSQ
                     PATGVRRQFLQRQPKTDGPEFRDVPILLEKLQLDVGQNLLDNGHHLYRAATLFPDDPG
                     ALKTAFGRYALGANVLKDTYRFFGVAPDTAGKLAVGTGILFKGLTFLREGELTLDFQF
                     DVGRGVKFETNLKLGVDPKNVTDVRKADVNFGFVRRF"
     gene            2112..2768
                     /locus_tag="VFY96_09750"
     CDS             2112..2768
                     /locus_tag="VFY96_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606787.1"
                     /translation="MTTFPGSPKLLKGGIVLIDPGTSAVLRIIALQYNPDTLMRTLQI
                     KALAEGGDRSDALRLTGPPVETIKLDAEIDATDQLEFPDQNQNAVQLGIHPQLAALET
                     IVYPANSQIQANNRLASAGTLEIIPMETPLALFIWSKNRVLPVRLTDFSITEEAFDPA
                     LNPIRAKVSLGMRVLSVNDLPFDHKGNSLFLSYHQQKESLAMKARGGSLSTLGITRIP
                     "
     gene            2776..3087
                     /locus_tag="VFY96_09755"
     CDS             2776..3087
                     /locus_tag="VFY96_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015129930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEX5606788.1"
                     /translation="MFTVTSRYYGIATASIERPNGEKINYVRRRFVAAPEQFALLQEH
                     AVTEGERLDQIATQYLGDPEQFWRICDANGAIRPDELVETIGRRLRITLPEGIPGTPN
                     A"
     gene            3080..4204
                     /locus_tag="VFY96_09760"
     CDS             3080..4204
                     /locus_tag="VFY96_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606789.1"
                     /translation="MLKGVYLTLLVGPVVPLPVPKIALEALTNVQVTTAAGSASGFQL
                     SFTLNNRSPLQTLFLIAGAQTPLLRVILIATINGNPNVLVDGVMTNQQVSPGGKPGES
                     TLTITGEDLTKVMALQDFSGIPYPAMPPEARVALIIAKYAVFGVIPLVIPSPFTDLPI
                     PVERIPIHQGTDLSYVRQLASNVGYVFYIDAGPVPGTNVAYWGPEIKIGVPQPALNFD
                     MDAQTNIESLNFSFNTADTVLPIVFIQNSLTKFPIPIPIPKLNPLQPPLGAIPAPITN
                     VTMLKETAKLSPMAAIGLGLAVAARSADAVSGTGTLDVVRYGRLLKARQLVGVRGAGT
                     AFDGLYYVKSVTTTMKQGELKQSFELTRNGLVSITPRVAA"
     gene            4201..4716
                     /locus_tag="VFY96_09765"
     CDS             4201..4716
                     /locus_tag="VFY96_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEX5606790.1"
                     /translation="MNSEQRYYGKYRGMVINNVDPLQIGRLQVQVSDIGGLIPSSWAM
                     PCFPLTGKQMGAYMIPQIGAGVWVEFEQGNPDYPIWSGCWFGSAAEVPALALAGNPAS
                     PNIVLQTALQNTIVVSDLPGPTGGIMLKSTTGASIIVNDTGIYIQNGKGASVVMLGPS
                     VTINNGALTII"
     gene            4811..5134
                     /locus_tag="VFY96_09770"
     CDS             4811..5134
                     /locus_tag="VFY96_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606791.1"
                     /translation="MPGFLLHFGASVLCAHAGQAQPTTVNPRVLVSGQPTVTITSPYV
                     VAGCALPPPPGANGPCITAQFVTSATRITSNGQPLLLLDSQAICAPTGTPLLILATQT
                     RVTGV"
     gene            5255..5617
                     /locus_tag="VFY96_09775"
     CDS             5255..5617
                     /locus_tag="VFY96_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEX5606792.1"
                     /translation="MSIDYPFRFDQRGRTAEADDDNHIRDLIEQVLFTAPGERVNRPD
                     FGSGLMRLVFAPNSDELAAATQFLVQGSLQQWLGDVIQVDEVSVVSDDSTLLVTVQYT
                     VRRTQQRQTAQFSRGGGF"
     gene            5617..8262
                     /locus_tag="VFY96_09780"
     CDS             5617..8262
                     /locus_tag="VFY96_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HEX5606793.1"
                     /translation="MIYFCCDDRRRRNAVAEHPFLNGIDFLEVSDNPADPIAIRQRTL
                     FVHFIKPLTFGSPSMSPPQSTLGIANMRIEGGERIRNIKVTRVSLAVAISPPVSSPPE
                     DDPHILKVEVSEPGDFSTYTLRLVHDPAHLNPPAGFDPVLSAVQFSFKVLCPSDFDCR
                     RERLCPEEPLHQPEIDYLAKDYASFRQLILDRMALLTPAWKERNPADLGIALVELLAY
                     VGDHLSYQQDAVATESYLSTARRRASVRRHVRLIDYPMHDGRNARVWVHVEVSAAGNG
                     LTLKRGKGSDTTKLLTHTDALSDRIVIPQDFDTPQKIAVSGARVFEPLHDIQLFSAHN
                     RMLFYTWGARECCLPAGATSATLAGAFPDLTPGDVLLLVESRGPQTGQIEDADVSHRH
                     AVRLNNVIVSTDPLGGRFKSPPDNNAVPVTQIEWNQGDALPFGLCISSRVGTGFYDDV
                     GIALGNIVLADHGMTVEDSSSASSLDPDTVPAENPALIRIDAAATNRCAGPTIEQMPS
                     RYRPQLKRAPLTQASPYDPTNPADTATAVLDLSFDDPAEFPAPAITLKNGADTAGAWE
                     PKRDLLASGPGDKQFVAEIETDGTVYLRFGDDQSGMRPVSGLQFFATYRIGNGSAGNV
                     GADTIRHVVTSDPDFASDLENPKITGLRNPLPARGGMDAETIEHARQNAPSAFGRQER
                     AVTPADYEQIAIRTDVVKRCGTDVQRAAATLRWTGSWHTVFVTVDRLRGKDVDAAFER
                     KLRGCLERYRMAGQDLEIDAPQYVSLEIEMAVCIKPGYFFEDVSEVLLEIFSNRTLPD
                     GRRGIFHPDNFSFGQPVYSSSLFAAAQQVAGVDSVALTKFQRQGIDSDVAENSGKLDI
                     GRLEIARLDNDPDFPEHGVFRLLAA"
     gene            8265..11009
                     /locus_tag="VFY96_09785"
     CDS             8265..11009
                     /locus_tag="VFY96_09785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HEX5606794.1"
                     /translation="MSAPDDIQINPAAASGDAVNDCGCCEGVSKETPSAIANRPGLSA
                     VAYRVGRHGQFKETMLARLSGSNYAALSALTTRDNDDFSIALLDAWATVADVLTFYQE
                     RIANEVYLRTATERFSVRELARLINYQLNSGVAASVYLAFSVESAPGALGEGLNPATG
                     AQPSTQRLPSIPIDTGTKVQSVPGPGELAQTFETVEPIAARAEWNAMKPRLTRPQQIS
                     ATAGFAIFQGTATNLKEGDALLIVDDTNEPALTRIIKLRIDDESKTTRADFTVAPSLP
                     PFEKPKPPPGRFDRFFGYVPLTNQVIREIASEKWSVGDLAALAKIQRWSTSDLFTNLA
                     KPPPPSEESAPLTTGIFALRQRAALFGYNAPKQPTYNGTVPKPPSEWDEWDLDTNEAA
                     NRIFLDAAYQAVSPKSYVAVDKPGKKPKIFQLDNTTVLPRTAYGISSKTTLLVLPTNQ
                     SWHDLLPARPDLGDLRDVVVHAQSEALALADLPIEDLIPETDGTITLDGIYNELETGQ
                     TVVVTGRWADLGSSVNSEVRKLEKVDIEARFTVLTFDQPLKQSYVRSTVTINANVALA
                     THGETVQEVLGGGDATQPFPRFSLKQPPLTYVPAANPTGAQSTLEVRVNDLLWHEVPS
                     FYDHDPEERIYITRTDDDGKTTVIFGDGATAARVPTGQENVRAKYRKGIGLGGLVKAD
                     RLTQLMTRPLGVKGVTNPSDATGAADPEVFEDARRNAPRTILTFGRIVSLKDYEDFAR
                     GFSGIAKALATPLWFGEKRGVFITVAGSAGAEVEDDSDLYKKLTTATKDAGDPRVPFR
                     IESYQPRLFRVSAAIKIDPDFEAQKISPQVEKNLREAFSFESRAFGQPVHLSELVAEI
                     QNTSGIVAVVVQEFYRSDQAPERRIRIPATIPKPGDEQPLAAELLTLDPRPLQLELMQ
                     "
     gene            11006..13279
                     /locus_tag="VFY96_09790"
     CDS             11006..13279
                     /locus_tag="VFY96_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606795.1"
                     /translation="MNFDVEKLYSLLPAIYRVRDDDVASQLALLLTFEEENELQQLAA
                     LGANASAEQSERRQELSDKKRRSGGPLKALLSIIAEQVAVLEDNLDQLYDDLFIETCA
                     EWVVPYIGDLVGARNLFAFSGGAFTQRAFVANTVAYRRRKGTATVIEQLARDVTGWSA
                     SVVEYFQLLTTTQYMNHLRPENLSIASVRHESFSGLYARSSSENSPYADDPQWKALEY
                     ANTTFDTLTHTADVRRIASRRGRYNIPNIGVFLYRLINFPVTQSSASRLDARRYFFDP
                     LGRDISLFNQPLAEAEISHLAEPLNVPMPISRRVLDRCFDTYYGLDAAGALKSLLLNV
                     NGTDVLPPEGSAVPVSPPELTASDLVVCNLSDVKDAGGNIIGWAHKPKNKIAIDPVLG
                     RIAFPENRPAPTTVTVSYHYGFSAAMGGGEYGRERTFIGSDPAVSVPEQSATIQQALD
                     QIAASGGVVEIRSSATFEETLTIRAATNQGKVIELRAADQHRPVLILSGDLTVFGADE
                     SEVTLNGLWISGSVRVPVADNSGNPNKLRLLRLRHCTIVPQLTVSNDSPPALTQPRIS
                     LEAPNTNIEIDHSIVGAIRAAAGADVAISHSIVDAAAQTEVAYAGISSGHPGAPLSIE
                     NSTIIGSVFTQTVQLASNTIFVSAVRAERLQQGCMRFSFVPPGSRVPRMHRCQPAASG
                     DAVRVRPVFSSLRLGDADYCQLSGQCPLEIRSGADDGAEMGAFHDLYQPQREANLRAA
                     LDEYLRFGLEAGIFFAT"
     gene            13310..>15339
                     /locus_tag="VFY96_09795"
     CDS             13310..>15339
                     /locus_tag="VFY96_09795"
                     /inference="COORDINATES: protein motif:HMM:NF039478.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6519 domain-containing protein"
                     /protein_id="HEX5606796.1"
                     /translation="MPGDYSRKTFHRSKHYSGVLMQQGRVQLDADWNEQLDIQLCRTE
                     TEALDVIGSSGVPQESDGFKIGIALGGRDLTISAGRIYVDGILCELEIPASYTTQPYY
                     PNPEFTAPGGSPPSSPPGGALDLVLDGGAYLIFLDAWQRELTALDDRLLREVALGGPD
                     TSARLQNVFQVRLLKIAPISPPQEINCQTAIPDFERLTALSTGKLNARTQPPTDDENL
                     CLLPPSAGYSRLENQLYRVEIHRSTTNLVTFKWSRDNASIETRIEKIEDNVLTVSDVG
                     KDEVLGFAAGQWVEIVDDESTLKGSPRPLLQIDTVDPAARQITLAGSAQDRAHLPNLK
                     LRRWDQIKDANADGVNVAQGNWVDLEGGIQVNFSGGSYRAGDYWLIPARTLTGEIEWP
                     PYEMPNTQPIPQPPRGIRHHYCRLGILEVDDNGVIDSVSDCRNVFPPLTEISAGCCTV
                     VVRPGDSIQAALDSLPDEGGCICLRTGEHEIGAPLRIEKSNVSLHGETLGARVTRREG
                     AELLKVAHPNGLLLENVTVSGIYFEFENKGVQVSGVPALIALDRCANSKVDGCIIRAQ
                     ELGTFAGTVIGRSTDVQVTNCRVEKARYGLWVVSDSTRLFLSDNVLDATDARKTDGGF
                     VGIFLMDAFGSSSIKENRITGFIFGIALNKGLFIGAPFSLASGSTITGNRIAR"
BASE COUNT         3228 a         4491 c         4562 g         3058 t
ORIGIN      
        1 gagagggcgg cggaacggga agcacgttta aagttggaag cgcttcgaat tttctcggtc
       61 ttagcccgct ggctgccgtc aggaaccggt tttatgacat tcacgcattt gcaaataagg
      121 gatccagtct cttgcacgtg atcgatcaac cgaaatgcga gatcgagtgc gagcagttct
      181 acgactgtgg caaccggcgc ttcggtcccg agttcgtgat cacgtattca atgacgcgag
      241 attcaatccc gcgagaaggc ggcggattta acgccgtgac gcgcgtgacc gtaagtaagg
      301 ccgcaaagtc ggcggcggct tcgccatccg gcagtggctc gacgccttga cggagtgaaa
      361 gttcaatgcg catgtcgcaa agcctctcac ttttttgcag agctacgggt gagacgatga
      421 gactcgatac tgtaattgcg ccgatcgaag tctaattcga tgaaccaaac cgctgcgcta
      481 caaacccgca ccgaaaaatc gccaccgagc agccggcatt cacagctcgt gcagcgtaag
      541 tgcggctgcg gcggctccag cggtgtgacg gggagctgca gcgagtgcga taagaaaaag
      601 cttctgggcc agtcgtttca agcgaggctg cgcatcaatg agccgggcga cgcctacgag
      661 caggaagcgg atcaggtggc ggcgcgcgtg atgcagatga ccgagcgcga tttgagactt
      721 ggcctgcggc aatccggcgg actggttcag cggcgcatta cgggccacga gctttccggg
      781 catctggtgc cgacgctcca acgccaagca gctgcgagtg agcccacgcc agccgaaggc
      841 agtccgcaat catccgatac gccgagtcaa aaagaagaag aggagagtag ttgtccaagc
      901 tggcgtggtg atccggagag catcagcaaa cgcgccgcgg agacctacgt tcgaaacgac
      961 atgacgccac catcgcaggc gacagtggag cgcatcgact gcgaaccgcc ggtgccgaac
     1021 ggcaactatg ggtgttatgt gcatttcagc gatggtctcg tgattcgcgt cattgtgcgc
     1081 gcgaaggaca ttgtcgtcgg cacggggccg ggacccttca caacgttgac gccgccacct
     1141 gcaacgccgc tgtgcttcta tgattacaca tgtccggaag gacaacttat tttgtccaag
     1201 cgcgaatgca agagcgccaa gccggcagga tcatcgggcc ccacgctggt cgggcagcgg
     1261 cgcgcggcgg cgggcgcttc gggaatgctc gacgccggac cgttcgtgga tgcggtgctt
     1321 gcttctccgg gaaagccgct ggatgcagcc gctgaggctt tcttttccgc ccgtttcggc
     1381 catgacttca ggaacgtgcg tgtccatgcc gatgccgcgg ccgccgagtc ggcgagggcg
     1441 gtgaatgcgc tggcatttac ggttggcaac cacgtggttt tgggtgccgg ccagtattcg
     1501 ccgcgcactc gggccgggca ggaactgctc gcgcacgaat taactcacgt cgtgcagcaa
     1561 ggcgccgcgg cgccgcgtgc cgccgagatc cacgaccagg gattctccgg cactccggcc
     1621 gtatcgagca gccaacctgc tacgggcgtg cgacgtcagt ttctccaacg ccaaccgaaa
     1681 accgacggcc ccgaattccg cgatgtcccg atactgctcg agaaacttca actcgacgtc
     1741 ggtcaaaacc tgctcgataa cggacatcat ctctatcgag ccgccactct gtttcccgat
     1801 gatcccggag ccttgaaaac cgctttcggc cggtacgcgc tcggcgccaa cgtgttgaaa
     1861 gatacgtatc ggttctttgg agtcgctccc gacacggcgg gcaagctcgc ggtcggcacg
     1921 gggatcttat tcaagggctt gacgttcctg cgggaaggcg aattgactct cgattttcaa
     1981 ttcgatgtcg ggcgcggcgt gaaattcgaa accaatttga aattgggagt cgatccgaaa
     2041 aatgtcaccg acgtccgtaa agccgacgtg aatttcggct tcgtgcgccg cttttaacac
     2101 ctgattgaat catgacgact tttcccggtt cacctaaact tctgaaaggc ggcattgtcc
     2161 tgatcgatcc cgggacttcc gccgtgctgc gcatcatcgc gcttcagtac aatcccgaca
     2221 cgctcatgcg gacgcttcag atcaaagcgc tcgcagaggg cggggatcga tcggatgcac
     2281 tgcgcctgac cggtccgccc gtggaaacga tcaagctcga tgcggagatc gacgcaacgg
     2341 accagttgga attccccgac cagaatcaaa atgccgttca gctcggcatt catccgcagc
     2401 tcgcggcgct cgagacgatt gtctatcccg cgaacagcca gatccaggcg aacaaccggc
     2461 tcgcgagtgc cgggacgctc gagatcatac cgatggagac tccgcttgcc ttgttcatct
     2521 ggagcaaaaa ccgcgtgctt cctgtgcggc tcaccgattt tagcatcacc gaggaagcct
     2581 tcgatccggc gctgaatccg attcgcgcca aggtgagcct cgggatgcgc gttctgagcg
     2641 tcaacgatct acccttcgat cacaaaggca acagcctctt tctgtcgtac caccagcaga
     2701 aggaaagttt ggcgatgaag gcgcgaggcg gaagcttgag caccctcggc atcaccagaa
     2761 ttccatgagg tgtttatgtt taccgtgacc agccgctatt acgggatcgc aacggccagc
     2821 atcgagcggc cgaacggcga aaaaataaat tacgtgcgcc ggcgtttcgt ggcggcgccc
     2881 gagcagtttg cgttgttgca ggaacacgcc gtgaccgaag gcgagcggtt ggatcagatt
     2941 gcgacgcagt atctcggcga cccggagcag ttctggcgca tttgcgatgc caacggcgcg
     3001 atccggcccg atgagctggt cgaaaccatc ggacggcggc tcagaatcac actgcccgaa
     3061 ggcatacctg ggacaccgaa tgcttaaagg cgtttatctg acgcttctgg tagggcccgt
     3121 ggttcctttg ccggtgccaa agatcgcgct cgaagcgctc acgaatgttc aggtcacaac
     3181 cgccgcggga agcgcgagcg gatttcagct gagtttcacg ctgaacaatc gctcgcccct
     3241 tcagacgttg tttctgatcg ccggcgcgca gacgccgctg ctgcgcgtga ttctcattgc
     3301 gacgatcaac ggtaatccca acgtgctcgt cgatggagtc atgacgaatc agcaggtgag
     3361 tccgggcggc aaaccgggcg aatcgacact cacgattaca ggtgaagacc tgacgaaggt
     3421 gatggcgctc caggacttca gcgggattcc gtatcccgcc atgccgccgg aagcgcgcgt
     3481 cgctttgatc attgcgaagt atgcggtgtt cggagtgata ccgctggtca ttccgagccc
     3541 gttcacggat ctgccgatcc cggtggaacg gattccgatt catcagggca cggatctgag
     3601 ctacgttcgc caactcgcgt cgaatgtagg ttacgttttc tacatcgatg cgggtccggt
     3661 gccggggacg aacgtcgcgt actggggtcc cgagatcaaa atcggtgtgc cgcaaccggc
     3721 gttgaacttc gacatggatg cgcagacgaa tattgaatcg ttgaatttca gcttcaacac
     3781 cgcggatacc gtgctgccga tcgttttcat ccagaactcg ctcacgaagt tcccgattcc
     3841 gattcccatt ccgaagctga atccactgca gccgccgctg ggcgcgattc cggcgccgat
     3901 cacgaacgtc acgatgctga aggagacagc gaaactttca ccgatggcgg caatcggact
     3961 cggccttgcc gtcgcggcgc gttccgccga tgccgtctcc ggcacgggca ccctcgacgt
     4021 ggtgcgctat gggcggctcc tcaaggcgcg gcaactcgtc ggcgtgcgcg gcgcggggac
     4081 agcgttcgac gggctctact acgtgaagag cgtcacgacg acgatgaagc agggcgagct
     4141 caagcaaagt ttcgagttga cgagaaatgg cttggtctcg attacgccgc gggtggcggc
     4201 atgaacagcg agcaaaggta ttacggcaaa tatcgcggca tggtgatcaa caatgtcgat
     4261 ccgctccaga tcggccgctt gcaggttcag gtgtctgata tcggcgggtt aataccttca
     4321 agctgggcga tgccgtgttt tccgctcacg ggaaagcaga tgggcgccta tatgattccg
     4381 cagatcggcg cgggagtctg ggtcgagttc gagcagggaa atcctgatta cccgatctgg
     4441 agtggctgct ggttcggttc cgctgcggaa gtgccggcgc tggcgctggc gggcaatccg
     4501 gcgagtccga atatcgtgct gcaaaccgca ctgcaaaaca cgattgtcgt gagcgatctt
     4561 cccggtccga cgggagggat catgctcaag agcacgacgg gcgccagcat cattgtgaac
     4621 gataccggga tttacattca gaacggcaaa ggtgcgtcgg tagtcatgtt ggggccttcg
     4681 gtgaccatca ataacggagc gctcacgatt atatgactcg ttcaaaccgt tccaaccgtc
     4741 ccggaggttc aaacggtttg aacggagcga agcgattgaa cggctggaac tgcttgaaca
     4801 aaggagttag atgcccggtt ttttattgca ttttggtgcc agcgtgttgt gcgcgcacgc
     4861 cggtcaggcg cagccgacga cggtcaatcc gcgggtgctg gtgagcggac aaccgaccgt
     4921 gacgatcacg tcgccgtatg tcgttgccgg ttgcgcattg ccgccgccgc ccggcgccaa
     4981 cggaccctgc atcaccgcgc agtttgtgac ttcggcgacc cggatcactt ccaatggtca
     5041 gccattatta ctgctcgata gtcaggcgat ctgcgctccg acgggaactc cgctgctcat
     5101 tcttgccacg cagacacgcg tcactggagt ttgaaacgcc atgaatatca cgtgttccgt
     5161 tctgctgctc gatagtcagg cgatctacgc ggcaatcgaa ctttcgctgc tcattatcac
     5221 cacgcagaca cgtcgcattg gagtttgaag gcccatgagc atcgattatc cgtttcgatt
     5281 cgatcagcgc gggcgcacgg ccgaggcgga cgacgacaat catatccgcg atctgatcga
     5341 gcaggtgttg ttcaccgcgc ccggtgagcg cgtcaatcgt ccggatttcg gcagcggcct
     5401 catgcgcctt gtctttgcgc caaacagcga cgagcttgcg gcagcaaccc agtttctcgt
     5461 gcagggatcc ctgcagcaat ggctcggcga tgtgatccaa gtcgacgaag tgagtgtcgt
     5521 cagcgacgat tcaaccctgc tggttacggt ccaatatact gtccggcgca cacagcagcg
     5581 tcaaaccgct cagttcagcc gcggaggcgg cttctgatga tttatttctg ctgcgacgat
     5641 cgccgccgcc gcaatgcggt tgcggagcac ccgtttctga acggaattga ttttctggag
     5701 gtgtccgata atcccgcgga tcccatcgca atacgccaga gaacgttgtt cgttcatttc
     5761 atcaagccgc tgacattcgg ttcgccgtcg atgagcccgc cgcaatccac attgggaatc
     5821 gccaacatgc ggatcgaagg cggcgagcgc attcgtaaca tcaaagtcac gcgggtgtcg
     5881 cttgcggtgg caatctcgcc accggtcagc tcgccgccgg aagatgatcc ccacatcttg
     5941 aaagtcgaag tttcagagcc gggggatttc tcgacctaca ctttgcgttt ggttcacgat
     6001 ccggcccatc tgaatccgcc ggccggcttc gatcccgtcc tgtcggcggt ccagttttcg
     6061 ttcaaggtcc tttgccccag tgactttgat tgcagacgcg aacggctctg tccggaggag
     6121 cctcttcacc aacccgagat cgactatctc gcgaaggatt acgcgagttt caggcaactc
     6181 atactcgatc gcatggcgct cctgacaccc gcgtggaagg agcgcaatcc agcggatctg
     6241 ggcatcgcgc tcgtcgagct gctggcctat gtcggcgatc acctgagcta tcagcaggat
     6301 gccgtcgcga cggaaagcta tctcagcacc gcgcggcgcc gcgcttcggt ccgccgccat
     6361 gtccgactga tcgactatcc catgcacgac ggtcgcaacg cgcgagtatg ggtgcatgta
     6421 gaggttagcg ctgcgggcaa cggtttgaca ctgaagcggg gtaaaggttc agacacgacg
     6481 aagcttctca ctcacacgga tgcgctttcc gatcgcatcg tgatccctca ggacttcgac
     6541 acacctcaga aaatcgctgt ctcaggagcg cgggtgttcg agccgctgca cgacatccag
     6601 cttttcagcg cgcataatcg gatgcttttt tatacctggg gcgcgcgcga atgctgcctt
     6661 cccgcgggcg ccacgagtgc cacattggcc ggtgcctttc ctgatttgac acctggggac
     6721 gttctgcttc tggtggaaag ccggggaccg caaaccggac agatcgaaga tgccgatgtg
     6781 agccaccgcc atgccgttcg actcaataac gtgatcgtca gcacggatcc tctcggcggc
     6841 cgcttcaaga gtccgccgga taacaatgcg gttccggtaa ctcaaatcga gtggaatcag
     6901 ggtgacgcgt tgccgttcgg gctttgcatt tcatcccggg tcggcaccgg attctacgac
     6961 gacgtgggaa ttgcgctcgg aaatatcgta ctcgccgatc acgggatgac ggttgaggat
     7021 tcgtcgagtg catcgtcact cgatcccgat acggttcccg cggagaatcc cgccctcata
     7081 cgcattgatg cggcggcaac aaaccgatgc gcgggaccga cgatcgagca aatgccgtcg
     7141 cggtaccggc cgcagctgaa acgagccccg ctcactcaag cttctccgta cgatccgacg
     7201 aacccggccg acacagccac cgcggtgctg gatttgtcat tcgatgatcc ggcggagttt
     7261 cctgctccgg cgattactct gaaaaacggc gccgatacgg caggcgcctg ggagcccaaa
     7321 cgcgatttac tcgcaagcgg gcccggcgat aagcagttcg tcgctgaaat cgaaacggac
     7381 ggaacggttt acctccgttt tggggacgat cagtcaggga tgcggcctgt gtccgggctg
     7441 cagtttttcg cgacctaccg gatcgggaat gggagcgccg gaaacgtcgg tgccgacacg
     7501 atccggcacg tcgttacgag cgatccggat ttcgcatcgg acttggaaaa tccaaaaatc
     7561 accggacttc gtaatccgct cccggcgcgg ggagggatgg acgccgaaac cattgaacac
     7621 gcgcggcaaa acgcgccgag cgcttttggc cgccaggagc gtgcagtcac gccggccgat
     7681 tatgaacaga tcgccatcag gacggatgtc gtcaagcgct gcggcaccga tgtgcaacgc
     7741 gcggcggcga ccctacgctg gacgggaagc tggcatacgg tgttcgtcac cgttgatcgt
     7801 ttgcgcggca aggatgtgga cgcggcattc gagcggaaac tccgcggctg cctcgaacgc
     7861 taccgcatgg cgggccagga tctcgaaatc gacgcgccgc aatatgtttc gctggaaatc
     7921 gaaatggcgg tctgcatcaa gccgggatat ttcttcgaag acgtgagtga ggtgctgctg
     7981 gagatcttca gcaatcggac cttgccggac ggccggcgcg gcatttttca tcccgacaac
     8041 ttcagtttcg gacaacccgt ctacagcagc tcgcttttcg ccgccgcgca gcaagtcgca
     8101 ggcgtggatt ccgtcgcgct aacgaaattc cagcggcagg ggatcgacag cgatgtagcc
     8161 gagaattcgg ggaaactcga catcgggcgg ctggagattg cgcgcctgga caacgaccct
     8221 gattttccgg aacacggcgt ttttcgtctc ctcgcggcat gaacatgagc gcacctgacg
     8281 acatacagat caatcctgcg gcagcttcgg gcgacgccgt gaacgattgc ggctgctgcg
     8341 aaggcgtgtc gaaggaaacg ccgtccgcta ttgcgaaccg tccggggctt tccgccgtcg
     8401 cttaccgcgt tggcaggcat ggccagttca aggagacgat gctggcgcgc ctctccggat
     8461 cgaattacgc cgccttgagc gcgctcacga cccgcgacaa cgacgatttt tccatcgcgc
     8521 tgctggatgc gtgggcaacg gtcgcagatg tgctcacttt ttatcaggag agaatcgcca
     8581 atgaagtcta cttgcgaacg gcgacggagc ggttctcggt tcgtgagctt gcgcgcttga
     8641 tcaattatca gctcaactca ggagtcgccg cgagcgtata tctcgcattt agcgtggaat
     8701 ccgcgccggg tgccttgggc gagggcctca acccggccac cggcgcgcag ccttcgacgc
     8761 agcggcttcc ctcgatccct atcgatacgg gaaccaaggt tcagagcgtt ccgggtcccg
     8821 gagaactagc gcagactttc gagacggttg aaccgatcgc agcgcgcgct gaatggaatg
     8881 cgatgaagcc gcgcctgacc cggccgcagc agatttcagc tacagctggg tttgcgattt
     8941 ttcagggtac cgcgacgaac ctgaaggaag gcgacgcgct gttgatcgtc gacgacacta
     9001 acgaaccggc tttgacgcgt atcatcaaac tccgaatcga cgacgaatcg aaaacgacgc
     9061 gtgcggactt caccgtcgct ccctctctgc ccccgtttga aaagccaaaa cctccgcctg
     9121 gacggttcga tagatttttc ggctatgttc cgctgaccaa tcaggtgatc agggaaatcg
     9181 ccagcgaaaa gtggagcgtg ggggatctcg ccgcactggc aaagattcaa cgctggtcca
     9241 cgagcgatct gttcacaaat ttagcgaaac cgccgccgcc gtccgaagag agcgcgcctc
     9301 tcacgacggg cattttcgcg ctccgccagc gtgccgccct gttcggctac aacgcgccga
     9361 aacagccgac ctacaatggc acagttccga agccgccatc tgaatgggac gagtgggacc
     9421 tcgacacaaa cgaagccgcg aacagaatct ttctcgacgc cgcataccag gcggtttcgc
     9481 caaagagcta cgtagccgtg gacaagcccg gcaagaagcc gaaaattttt cagctcgaca
     9541 acacgacggt gcttccgcgc accgcctacg ggatcagcag caaaacaaca ctgctggtgc
     9601 tgcccacgaa tcaatcctgg cacgatctct tgcccgcgag gccggatctg ggagatctcc
     9661 gtgacgttgt cgttcatgcc caaagcgagg cgctcgcgtt ggccgacctg ccgattgagg
     9721 atctgattcc cgagaccgac ggaaccatca cgctggacgg catctacaat gagctcgaaa
     9781 ccgggcaaac cgttgtcgtt acgggccggt gggcagatct cggcagctcc gtcaacagcg
     9841 aagtgcggaa actcgagaaa gtcgacatcg aggcgagatt caccgttctt acgttcgacc
     9901 agcctctcaa gcagagctac gtccgaagta cggtcacgat caacgccaac gtcgccctcg
     9961 caactcacgg cgagacggtt caggaggttc tcggcggcgg cgatgcgacc cagccctttc
    10021 cgcgctttag cctcaaacaa ccgccgctca cctatgttcc cgcggctaat ccgacaggcg
    10081 cgcagagcac actcgaggtt cgcgtaaatg atctcctatg gcacgaagtg ccttcctttt
    10141 atgaccatga tccggaggaa cgaatttaca tcacacggac cgacgacgac ggcaagacaa
    10201 ccgtgatttt tggggacgga gcgaccgcag cgagggtgcc gaccgggcag gagaacgtca
    10261 gggcgaaata ccgcaagggg atcggactcg gagggctggt caaggcggac cggctcaccc
    10321 aattgatgac gcggccgctc ggagtgaagg gtgtcaccaa tccttcggat gcgaccggag
    10381 ccgccgatcc ggaagttttc gaagacgccc gtcgcaatgc gccgcgcaca atcttgactt
    10441 ttggccggat cgtttccctc aaggactatg aagattttgc gcgcggcttc agcggcatcg
    10501 ctaaagctct ggcaactcca ttatggtttg gcgaaaaacg gggcgtcttc attacggttg
    10561 caggatcggc cggggcggag gtcgaggacg acagcgacct ttataagaag cttacaaccg
    10621 cgacgaaaga tgcgggcgat ccgcgcgtgc cgttccggat cgaatcgtat cagccgcggc
    10681 tgttccgcgt ttcggcagcg atcaagatcg atccggattt cgaagctcaa aaaatctcgc
    10741 cgcaggtgga aaagaatctg cgcgaggcct tctccttcga gagccgcgcc ttcggtcagc
    10801 cggttcatct gagtgaactc gtcgccgaga tccagaacac atccggcatc gttgccgtcg
    10861 ttgtgcagga attttaccgt tcggatcagg cacccgaacg gcggatccgg ataccggcaa
    10921 cgattcccaa acccggcgat gagcaaccct tggcggccga gcttctgacc ctcgacccgc
    10981 ggccccttca gctggagcta atgcaatgaa tttcgatgtc gaaaaacttt actccctgct
    11041 gccggcgatc taccgggtga gagacgacga tgtcgcgtct cagctggcgc tgctcctcac
    11101 tttcgaggaa gaaaacgagt tgcagcagct cgcggcgctg ggtgcgaatg cgagcgcgga
    11161 gcaaagcgag cgtcgccagg agctttcaga caagaaacgg cgcagcggcg gaccgctgaa
    11221 agcgctgctt tcgattatcg ccgaacaggt ggcggtgctc gaggataatc tcgaccagct
    11281 ttacgatgat ctgttcatcg aaacctgtgc cgagtgggtc gtgccataca taggcgatct
    11341 cgtgggcgcg cggaatctgt tcgcattttc cggtggagct ttcacgcaac gggcatttgt
    11401 cgccaatacg gtagcgtacc gccggcggaa agggacagcg acggtgatcg agcaactcgc
    11461 gcgcgacgtc accggatgga gcgcgagcgt tgtggaatac tttcagttgc tcacgacgac
    11521 acagtacatg aatcatcttc ggccggagaa tctttcgata gcgagcgtgc gccatgaaag
    11581 cttcagcgga ctttacgcac ggtcctcgag cgaaaattcg ccgtacgccg atgatccgca
    11641 atggaaggcc cttgaatatg ccaatacgac cttcgacacg ctcacacaca cggcggacgt
    11701 gcgccggatc gcatcgcgcc gcggccgtta caacatccca aatatcgggg tttttcttta
    11761 tcgtttgatc aacttcccgg tgacgcaatc gtcagcatcc cggttggatg caaggagata
    11821 tttttttgat ccgttgggca gggatatctc gctgttcaat cagccgctcg ccgaagccga
    11881 gatcagtcac ctcgcggaac cgctcaatgt tcccatgccc atcagccgcc gcgtactgga
    11941 ccgctgtttc gatacatact acggactgga cgctgccggc gccctcaaga gcctgctgct
    12001 caacgtgaac ggcacagacg tgctgccgcc cgagggctcc gcggttcccg ttagtccgcc
    12061 cgagctgacg gcgtcggatc ttgtcgtctg taatctcagc gatgtgaaag atgccggcgg
    12121 caacattatc ggctgggcgc ataagccgaa aaataaaata gccatcgatc cggttttggg
    12181 gcggattgct tttccggaga atcgccccgc tccgacgact gtgaccgtca gctatcatta
    12241 tggattcagc gcggcaatgg gcggcggcga atatgggcgg gaaaggacct ttatcgggtc
    12301 cgatcccgcg gtgtcagtgc cggagcagag cgccacgatc cagcaggccc tcgatcaaat
    12361 cgccgcgtcg ggcggtgtgg tcgaaatcag aagcagtgct acgttcgagg aaacactcac
    12421 gattcgagcc gccactaacc agggcaaggt catcgagctc agggctgccg atcagcaccg
    12481 tccggtgttg atcctgtcag gcgatctgac agtcttcggc gccgatgaat cggaagtcac
    12541 gctcaacggc ctgtggatca gcggcagtgt ccgggttccg gtcgcggata acagcgggaa
    12601 tcccaacaag ctccggctgc tgcgattacg ccactgcacg atcgtgccgc aattgacagt
    12661 ttccaacgat tctccgcccg ccttaacgca accccgaatt tccttggagg cgccgaatac
    12721 caacatcgaa atcgatcact cgattgtcgg tgcaatccgt gctgctgcgg gagcggacgt
    12781 cgcgataagt cacagcatcg tcgatgcagc cgctcaaacg gaagtcgcgt atgcgggaat
    12841 ctccagcggc catcccggag cgccgttgag tatcgaaaac agcacgatca tcggaagtgt
    12901 tttcacccaa accgtgcagc tcgcgtcgaa tacgatattt gtttccgccg ttcgcgccga
    12961 gcgattgcag cagggctgca tgcgtttttc gtttgtgccg ccgggctcgc gcgtaccaag
    13021 gatgcatcgc tgccagccgg ccgcttccgg cgatgcggtt cgggtccggc ccgtcttcag
    13081 ttcacttcgg ctcggggatg cagactactg tcaattgagc ggtcaatgcc ctttggaaat
    13141 ccgcagcggc gccgacgacg gcgcagagat gggcgcgttt cacgatcttt accagccgca
    13201 acgcgaggcg aatcttcgag ccgcactgga cgaatacctg cgctttgggc tggaagcggg
    13261 aatttttttc gcaacgtgac cgaacaccgc aaagagccag gagaacaaaa tgccgggtga
    13321 ttacagccgc aagacctttc atcgcagcaa acactatagc ggcgtgctca tgcagcaggg
    13381 gcgagtccag ctggacgccg actggaatga gcagcttgat attcaacttt gccgcactga
    13441 gaccgaggcg ctcgatgtca ttgggagctc cggcgttccg caggagagcg acggctttaa
    13501 aatcggcatc gcgctgggtg gccgtgatct gactatttcg gcaggacgga tctacgtcga
    13561 tggcatcctc tgtgaattag aaattcctgc aagctacaca actcagccat attatcccaa
    13621 tcctgaattt acggcaccgg gcgggagccc gccttcgagt ccgccgggcg gtgctctcga
    13681 tctggtgctg gacggtggcg cttacctcat ttttctcgat gcgtggcagc gagaactaac
    13741 cgctctcgac gaccggctgc ttcgcgaagt ggcgctcggc ggaccggata cgagcgctcg
    13801 tctgcaaaat gtttttcaag tcaggctgct gaagatcgcg ccgatcagcc cgccgcagga
    13861 gataaactgc caaaccgcca ttccggattt tgagcggctc acggcgctta gcaccgggaa
    13921 attaaatgct cgtacccagc cgccgaccga cgacgaaaat ctttgtctcc tgccgccgag
    13981 cgccggttac agccggctgg aaaatcagct ctaccgcgtc gagatacatc gatcgaccac
    14041 aaatctggtg acgttcaagt ggtcgcgcga caacgcttcc atcgaaaccc gaatcgaaaa
    14101 gatcgaggat aatgtcctca cggtttcgga cgtcggcaaa gacgaagtcc tcggctttgc
    14161 ggcgggtcag tgggtcgaga tcgtcgacga cgaatccacc ttgaaaggtt cgccgcggcc
    14221 gttactgcaa atcgacactg tagacccggc agcccgccag ataacacttg ccggctcggc
    14281 tcaggaccga gcccatctgc cgaaccttaa gcttcggcgc tgggatcaga tcaaggacgc
    14341 gaatgcagac ggagtcaatg ttgcgcaggg taactgggtc gatctcgaag gcggcattca
    14401 ggtgaatttt tccggcggga gctaccgcgc cggagactac tggctgattc cggcccgcac
    14461 actcaccggc gagatagaat ggcccccgta cgaaatgccg aacacgcagc cgattccaca
    14521 gccgccccgc ggtattcgcc atcactactg ccgcctcggg atcctcgaag tagacgacaa
    14581 cggtgtcatt gacagtgtct ccgattgtcg gaacgtgttc ccgccgctga ccgaaatatc
    14641 ggcgggctgt tgcaccgtcg tggttcgtcc cggcgacagc attcaggccg cattggattc
    14701 gctgcctgat gaaggcggat gcatttgtct gagaaccggc gagcatgaaa tcggcgctcc
    14761 gttgcgcatc gaaaaatcca atgtctcgct gcatggggaa acattgggtg cgcgcgtgac
    14821 gcgcagagaa ggcgcagagc tcttgaaggt cgctcacccg aacggtttgc tgctagagaa
    14881 cgtcacggtc agcgggattt atttcgaatt cgaaaataag ggcgtgcagg tgtccggcgt
    14941 ccccgcgctg atcgctctcg atcgctgcgc taacagcaag gtcgacggtt gtatcattcg
    15001 tgcccaggag cttgggacat tcgcaggtac ggtcatcggc aggagcaccg atgtgcaggt
    15061 cacaaactgt cgtgtcgaaa aagcgcgtta cggcctttgg gtggtgagtg attcgaccag
    15121 gcttttttta tccgacaatg tcttagatgc gacagatgcc aggaaaaccg acggcggctt
    15181 tgtcggcata tttctgatgg acgcgtttgg ctcctcgagc ataaaagaga atcgaatcac
    15241 tgggtttatt ttcggcattg ctcttaacaa aggacttttc atcggtgcgc cgttctcgct
    15301 cgcgagcggg tcgacgataa ccggcaatcg tatcgcgcg
//
LOCUS       DASULZ010000479         2410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1175190, whole genome shotgun sequence.
ACCESSION   DASULZ010000479 DASULZ010000000
VERSION     DASULZ010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175190"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VFY96_09800"
     CDS             complement(<1..192)
                     /locus_tag="VFY96_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006210056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5606797.1"
                     /translation="MKSLQDKIVVVTGASRGVGLQLARTFALEGSRVAMAARDQRTLA
                     DAAEKIADSGGHTLPFACDV"
     gene            339..1409
                     /locus_tag="VFY96_09805"
     CDS             339..1409
                     /locus_tag="VFY96_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5606798.1"
                     /translation="MKLSIILALSLLFLIAPCTSAETNIADKHSFTWQEAKPSAETEL
                     DRLSQAFVQLADHSRPAIAQIRVTTQDGKGTSTQAFGSRGSGFIIDPTGYILTAHHVI
                     DKAKAIEVRLPDSRRLAAQLVAADAQIDIAILRVQVDAPLPIISMGDSDTIRVGNLAA
                     VFGYPFGRESSMSLGIISRAGRSYRDSASFEYIQTDAGAYPGGSGGPLLNSSGQVIGI
                     ITMASERGNMGFATPINVIKRMLPRLASGEKLAWGWLGVQMSDISLDHAKALGLHPVK
                     GVVISSVLPGQPAARGGLQKQDVILSVNENYVETPRDVARMVGGLEAGRVVHLTILRN
                     GKTLQLSFPLGIRPESTKSREG"
     gene            complement(1417..1674)
                     /locus_tag="VFY96_09810"
     CDS             complement(1417..1674)
                     /locus_tag="VFY96_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606799.1"
                     /translation="MSFVIRSSSGKFPLARKSSAKGNRAFTRSVPSMKGIEVLRLPAG
                     KQDEDGAYPEGSVNHRVLKKLQEYSDRQKKFGSPAERKNVA"
     gene            1828..>2410
                     /locus_tag="VFY96_09815"
     CDS             1828..>2410
                     /locus_tag="VFY96_09815"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX5606800.1"
                     /translation="MVSLTLLIAACSSLVAPVIPSATGPSEDEETKISREFRREAKKF
                     LKFVNNPEVERYVDRIGRRILAATGPQSFEYRFFVVEDPQLNAFAVPGGSIYVYTGLI
                     ERAKTTDELAGVIGHEITHVKGHHMARSSGPDAISILSLLSMVALARTGSGAQAAGMV
                     GQAVAATRQLAYSRQLEMEADTLGARYMAAAGYD"
BASE COUNT          501 a          686 c          668 g          555 t
ORIGIN      
        1 cacatcgcac gcaaacggca gcgtgtgtcc tcccgagtcg gcgatttttt ccgctgcgtc
       61 agctaaggtg cgctgatcgc gggctgccat tgcgacccgg gaaccttcta gcgcaaaggt
      121 ccgagccaat tgtaagccca cgccgcggct tgcaccggtc accaccacga ttttgtcctg
      181 taagcttttc atacagagag aaagaatagc agattcagag gtttgaagac caatctgtgg
      241 cggcttgccc ggtgccccag tgaagctcgg cctaggcagc ttgctctgca aaaagttttt
      301 cgataaagca cagccgttaa tcaggccgga ggtaagccgt gaagctctcg atcatccttg
      361 ctttgagtct cctatttctg atcgcccctt gtacgtcggc cgaaaccaac atcgccgata
      421 aacactcctt cacatggcag gaggccaagc cgagcgccga gacagagctt gaccgattga
      481 gccaagcatt cgttcaactg gccgaccatt ctcgaccggc catcgctcag atccgcgtta
      541 ccacgcaaga tggaaagggc acatcgacgc aggctttcgg tagccgcggt tccggtttta
      601 tcatcgaccc gacgggttat attttgaccg ctcatcatgt gatcgacaag gccaaggcga
      661 ttgaagtgcg attgccagac tctcgcaggc tcgccgctca actggttgcg gcggacgcgc
      721 agatcgacat agcgatatta agagtgcagg ttgacgcccc gttgccgatt atctcgatgg
      781 gcgactcgga taccattcgc gtcggcaacc tcgccgcggt ctttggttat ccctttggtc
      841 gcgagagttc catgagcttg ggaatcatca gccgcgccgg ccgtagttat cgtgactcgg
      901 caagtttcga gtatatccaa acggatgcgg gagcgtatcc cggcggtagc ggcggccctt
      961 tgctcaacag ctctgggcag gtaatcggga tcattaccat ggcttcggag cggggcaaca
     1021 tgggattcgc gacaccgatc aacgtcatca agcgtatgct tcctcgcctg gcaagcggcg
     1081 aaaaactggc gtggggctgg ctgggagtgc agatgtccga catctctctc gatcacgcga
     1141 aggcgctcgg gttgcatccg gttaagggcg tggtcatcag ttcagtgctg cctggccagc
     1201 ctgccgcccg cggaggctta caaaaacaag atgtgatttt gtcggtcaat gaaaattatg
     1261 ttgaaacgcc ccgcgatgtg gcacgcatgg ttggtggttt ggaagcgggg cgagtggtac
     1321 atctgacgat tctccgtaac gggaaaactt tgcaactgtc gttcccgctc ggcattcggc
     1381 ccgaatcgac caaatcccga gaaggctgag gttgcgctag gccacgtttt ttcgctcggc
     1441 cggtgatccg aattttttct gccgatcact gtactcttgc aatttcttca gcacgcggtg
     1501 gttcacggat ccttccggat aagcaccgtc ctcgtcctgc ttgcccgcgg gcagcctcaa
     1561 gacttcgatt cccttcatcg atggtaccga ccgcgtaaat gcgcgattcc ctttcgcgct
     1621 ggatttgcgc gccaacggga attttccgct ggagcttcga ataacaaacg acatgattag
     1681 ctgctctgaa ttaagtcgtt gacatatgta tcgcacaaag ctcatcaact ctccgtcaac
     1741 gttgaatggc ctggtgttag tctctatact ctatcaatgt ttcgtcccat cgctttgcgc
     1801 tgctcttgcc gtacttattg cgcctgggtg gtctcgctta ctctcctgat tgccgcctgc
     1861 agttcgctag tggcgccggt gattccgtcg gcgacaggcc cgagcgaaga cgaagaaacc
     1921 aaaatcagtc gggagtttcg ccgcgaagca aaaaaatttc tcaagttcgt caacaaccct
     1981 gaagtcgagc gttacgttga ccggattggc cggcgcattc ttgccgccac cggaccacaa
     2041 tcgttcgaat atcgattttt tgttgtcgaa gatcctcagc tcaacgcctt tgccgttcct
     2101 ggcgggtcca tctatgtcta cacggggctg atcgagcgag cgaaaaccac cgacgagctc
     2161 gccggtgtca tcggtcacga aattacccat gtgaagggac atcacatggc gcgctcctcg
     2221 gggcccgatg ccatttcaat attaagcctg ttgagcatgg tggcgctggc tagaaccggc
     2281 agcggcgcgc aagcagcagg catggtaggt caagcggtcg cggccacgcg ccaactcgcc
     2341 tatagtcgcc aactcgaaat ggaagcggat accctcggtg cgcggtacat ggctgccgct
     2401 ggctatgatc
//
LOCUS       DASULZ010000480         2286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1378561, whole genome shotgun sequence.
ACCESSION   DASULZ010000480 DASULZ010000000
VERSION     DASULZ010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1378561"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VFY96_09820"
     CDS             complement(<1..320)
                     /locus_tag="VFY96_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX5606801.1"
                     /translation="MAKPMSRVSEAQLIPISFTLNGRLQEIEVQPHELLLDVVRERLG
                     LTGAKRSCDVEVCGACTLLVDGRPVSACTTLAFEVRGRSVLTIEGLAENGKLHPLQQA
                     FIEHG"
     gene            complement(346..1191)
                     /locus_tag="VFY96_09825"
     CDS             complement(346..1191)
                     /locus_tag="VFY96_09825"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX5606802.1"
                     /translation="MLRRFRLEEPESVSEVSELLGRFGDSAKIYAGGTELLLAMKEGL
                     VQYERLINVKKLKCKDVILENGALSIGALCTHRELETSEVIKEKLPALAKLEHNVANV
                     RVRQVGTIGGNLCFAEPHADPGTLLIALGAKMIAEKPSAQREIQAEEFFVDAFETTLE
                     PDEVLTKIVIPAPAPVGRSAYLKFGYLERPSVGVGLAFNLNGQVSSMRIAVGCAGPAP
                     RRVPEAEALLEGKSREEAERNLAQAGALAGRAAQAMTDLHGSQDYKEHIVGVLLKRAF
                     LSALS"
     gene            complement(1193..1645)
                     /locus_tag="VFY96_09830"
     CDS             complement(1193..1645)
                     /locus_tag="VFY96_09830"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX5606803.1"
                     /translation="MQFQKEVEIRAPREKVWRFIWDVDRFIACVPGCKEAKTLEEGKR
                     YAATMTEKVGPFKVEFPTNIEVLEREELTHIKAQASGADNKIGSRMKLDLDVNLRENG
                     DKTVLAFVAGVDILGKLAALGHGIIKRKADQVLDEFAQAVKKQLEGDS"
     gene            complement(1794..>2286)
                     /locus_tag="VFY96_09835"
     CDS             complement(1794..>2286)
                     /locus_tag="VFY96_09835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5606804.1"
                     /translation="QAQRKLFFNLQKEKEKIEAEGLTPGPKLLAQRLQVKEDEVVEME
                     QRLASRDLSVDVPIGDDDEATLLNFLQDSKHTPEEQIAESEYRELLRDKMEQFANTLK
                     DKELVIYRERLLNEDPVTLREIGEKYGISRERVRQIEERVKKKLKTYLKDIRNVDDAS
                     GAR"
BASE COUNT          480 a          689 c          546 g          571 t
ORIGIN      
        1 ccatgttcta tgaaggcttg ctgcaaggga tgaagcttgc cgttttccgc caggccttcg
       61 atggtcagaa ctgagcggcc acgaacttcg aatgccaacg tagtgcacgc gcttaccggt
      121 cgaccatcga ccagcaaagt gcacgcgccg caaacttcta catcgcacga tcgcttggcg
      181 ccggtgagac ctagccgttc gcgcacaaca tcgagcaaca gttcatgcgg ttgcacctcg
      241 atttcctgca agcggccatt caaagtgaaa gagattggaa tcaactgcgc ttcggaaacc
      301 ctactcattg gtttggccat gaattcttct ctccaatccg ccgcatcagc tgagcgcgct
      361 cagaaaagcc cgcttcaaaa gaacgccgac gatgtgttct ttataatcct gcgagccgtg
      421 caaatcggtc atcgcctgag ccgctcggcc ggcaagtgcg cctgcctggg cgagattgcg
      481 ctcggcctcc tctctgcttt tgccctcgag caatgcttca gcttctggga ctctgcgcgg
      541 cgctggtccg gcgcatccga ccgcgatccg cattgaactg acctgcccat tcagattgaa
      601 cgccaagccg actccgacac tcggccgctc gagatagcca aatttgagat aggcactacg
      661 cccgaccggc gcgggagccg gtatgacaat cttcgttaag acttcgtcgg gttccagcgt
      721 tgtttcaaac gcgtcgacaa aaaactcctc cgcttggatt tctcgttggg cggaaggttt
      781 ttcggcaatc attttagcgc cgagggcgat cagcagggtt cccggatctg catgaggttc
      841 ggcaaaacaa agattcccgc cgatggttcc cacctgccgc acccgcacat tggcaacgtt
      901 gtgctcgagc tttgccaacg cgggcaattt ctctttgatt acctctgaag tctccagttc
      961 acgatgcgta catagggcgc cgatcgacag tgcgccattt tccagaataa catccttgca
     1021 cttgagtttt ttcacattaa ttagccgctc gtactgaacc agtccttctt tcatcgccag
     1081 caacagctct gttccgccgg cgtaaatttt cgcactatca ccaaagcggc cgagcagctc
     1141 ggaaacttcg gacacacttt ccggctcttc caagcgaaac cttctgagca ttctacgaat
     1201 ccccttccag ctgcttcttc acggcctggg caaactcgtc caaaacttga tccgctttgc
     1261 gcttaataat cccgtgtccc aaggcggcca actttcccaa aatatccacg ccggcaacaa
     1321 atgcgagaac ggttttatcg ccgttttccc ggagattcac gtcgagatcc agcttcatcc
     1381 ggctgccgat tttattgtcg gcaccggatg cctgagcttt gatgtgcgtc agctcttcgc
     1441 gctcgagcac ttcgatgttg gtcggaaatt cgactttgaa cggaccgacc ttctccgtca
     1501 ttgtcgcggc gtaccgcttt ccctcctcga gcgtcttggc ttccttgcat ccgggaacgc
     1561 aagcgatgaa gcgatcaacg tcccagataa agcgccaaac tttctcgcga ggcgcgcgaa
     1621 tttccacttc cttttgaaat tgcaacgggt cttcctccga atcgaatgtc cttttttgct
     1681 atcagaagcc tccgaaccgc gcaagcaaag cccaactgaa gatcaatgtc cccgaatcat
     1741 gaccgggcaa gagtgactga ggcaggcgcc gcgtcggagt aaggacagaa aaactagcgg
     1801 gcgccagacg cgtcgtcgac gtttctgata tccttgaggt aagtcttgag cttttttttc
     1861 acccgctctt cgatctgccg cacccgctcg cgactgatgc cgtatttttc cccgatctcg
     1921 cgcagggtaa caggatcctc attcaagagg cgttcgcgat aaatcaccag ttctttatcc
     1981 tttaaagtgt ttgcgaactg ctccatcttg tcgcgcaata actcgcgata ttcgctttcg
     2041 gcaatctgtt cttcgggcgt atgtttacta tcctggagaa aatttagcag cgtcgcttca
     2101 tcatcatcgc cgatcggcac atccacggaa aggtcgcgac tcgcgagccg ttgttccatt
     2161 tccacgactt catcttcctt cacctgcagc cgctgagcta acaatttcgg tcccggcgtg
     2221 agaccctcgg cttcgatttt ttccttttct ttctggagat tgaaaaatag ttttcgttgc
     2281 gcctgc
//
LOCUS       DASULZ010000481         3456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1479004, whole genome shotgun sequence.
ACCESSION   DASULZ010000481 DASULZ010000000
VERSION     DASULZ010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479004"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VFY96_09840"
     CDS             <1..247
                     /locus_tag="VFY96_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5606805.1"
                     /translation="LPEQITLGTGNPRQMPVLDRIGLDRAIAQLPRGYRTVFVLHDVE
                     GYEHEEIARMLDRSVGTSKSQLHKARMKLRGLLKQRG"
     gene            836..1468
                     /locus_tag="VFY96_09845"
     CDS             836..1468
                     /locus_tag="VFY96_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606806.1"
                     /translation="MNQMSGKRLSGLTVLVGILTLVFSPASVFAHGGEDHDQKPQTVS
                     PGTNMIVHVGRAGDFELVIKHAPIEPDKEMVARVFVSRFANNEPVSGAKAIVTLTSEG
                     SAPVELTAAAGATPGMYEVKLPPLPKGHYKLAARIDSNGEIKPATLSALEVAPQPVTA
                     TGAVATWARTALIVMGVFVFLSIIGFLGVVIYRSSRASRREHIEGETAAA"
     gene            1516..3126
                     /locus_tag="VFY96_09850"
     CDS             1516..3126
                     /locus_tag="VFY96_09850"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX5606807.1"
                     /translation="MTQLTNLIRRRSRAIVVVALFASFAQAQTPPQGPPRENLSPAQT
                     PTPPQPGTVRPTTMTTGVPPVQLSSSQYIDPAGRTVEQLFEAGFTRRADLLAARQRLS
                     IARGRLLQAGLRPNPVLDSEYTSARILGGQDEGDFSVGVSQLFETGGKRSKRVTVARL
                     ELAQVQAEVRALERQFAADLRAAYARALAAARQLDTEEKLIAANEEIVRVTTARLNEG
                     DVAPLDLNLVRVETDRLRAQTVRTRADLEGQLISIRTFAGFEVTEPLRIAPLPEKPPR
                     LDLSLPEATDMALRERADLQAARLGEEVGSARIQLAQAQAVPNLALTSRYSIRRDIFD
                     DTPVGILRDKDRLLTFGVSVDIPVFNRNQGEIVSAVGEKTQAQRQREFLEATIKRDVA
                     LAYRRYRAATEALVLYATQIIPIAEKNVQAVRAAYNAGEFSVFDVIAEQRRLIENETA
                     YNESLGEYYTSLAELERALGITLPPSAFAPGTITVVPDIDRLTGGKLVQSIKSTMLMD
                     QPIANGGKLPLSGPAVTNSQTERIVPQN"
     gene            3156..>3456
                     /locus_tag="VFY96_09855"
     CDS             3156..>3456
                     /locus_tag="VFY96_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606808.1"
                     /translation="MKGLLTLLLASLLAGFAAACATSENTNTAPATSTANTAPKPAAK
                     DDVPPSVHAAFPDAQSITKDHKDLTTAQIAEIEKEAGSKLPDTDHHAYLAFSTSGG"
BASE COUNT          783 a          882 c         1050 g          741 t
ORIGIN      
        1 cctgccagag caaattacac tcggcactgg taacccgcgg cagatgccag tgcttgatcg
       61 cattgggttg gatcgcgcaa tcgctcaatt accacgcggc tatcgcacgg tgttcgtgct
      121 ccacgatgtc gagggatacg aacatgaaga gatcgctaga atgctggatc gttcggtagg
      181 aacctcgaag tcgcaattac ataaggcgcg catgaagttg cgagggttgc tcaaacagcg
      241 aggctgatta tgggcgttat gaaacgcagg actgcatcca gttggtgaaa cagatcacgg
      301 tgattggctg actgggaggc gcagtagttg cttggctgga ctaggaccct ggagatttag
      361 caaatggaaa ccctgaacta agagtcccca accgatgccg aagttcgata tggatagaag
      421 cagagaaatg tttttcaagt gtcctgtggg cggattcgct tcgcttagtc ccggcatttg
      481 cccaaagtgt aaggcgcagc tcgtcgcggt ttcggttaga gagtcgtcag cagccgcagg
      541 gcgcggtcgt agatcgcaat caggaccggc gaagctcgaa gcgatgaagg cgagttagaa
      601 ccgtctgtta acgcacaggt cgctgtctgc aaacagacaa tttggaggaa ggcgcatagg
      661 gaaattcctc aagtattcaa cagtcactgc actctatcgg gaagctttgg ccttaaggtt
      721 ttgggcacga cgattgcgct gcaaaccatt tcaatgttcc gcaaatggtt gtcttagtgt
      781 gcttgaacga attgagccga gatattgccg tgatgttccc ataggagctt tagatatgaa
      841 tcagatgtct ggaaaaagat tgagtggtct aacagtgctg gtcgggatct taacactcgt
      901 tttctcacca gcttcagtct tcgcgcatgg tggtgaggat catgaccaaa aaccgcaaac
      961 ggtctctccg ggcacgaaca tgatcgtaca tgtcgggcga gccggtgatt ttgagcttgt
     1021 gatcaagcac gcgccgatag agccggacaa ggaaatggta gcgcgagtgt ttgtgagtcg
     1081 ttttgcaaac aacgaaccgg ttagcggcgc gaaagcgatc gttactctca cgagcgaagg
     1141 cagcgcgccg gtcgagttga cggccgctgc cggtgcaacg cccggcatgt atgaagtgaa
     1201 gctgccaccg ttgccaaagg gacactacaa actggccgcg cggattgatt caaacggaga
     1261 gataaaaccg gcgactttga gtgcacttga ggtggcaccc caacccgtta cggcaaccgg
     1321 cgcggtcgcg acgtgggcgc gtacagcttt gatcgttatg ggggtgtttg ttttcctgag
     1381 catcatcggg ttcttggggg tggtcattta cagatcatcg cgggcatcgc gccgtgaaca
     1441 cattgagggg gagacggccg cagcctaagc gtttgggccg aatcggctac agagcgtatt
     1501 gcctgctaac ggatcatgac ccagctaacg aatttgattc ggcgccgctc gcgcgcgatt
     1561 gtggtagtgg cgctgtttgc gtcatttgcg caggcgcaaa caccaccaca gggaccacca
     1621 agggagaact tgtcgccggc gcaaacgccg actccaccgc aaccgggaac ggtgcggcca
     1681 acaaccatga ctactggtgt tccgccggtt cagctttcgt ctagtcaata catagatccc
     1741 gcaggccgga cggtggagca attgttcgaa gccggattca ctcggcgggc cgatttactg
     1801 gctgcgcgcc aaaggctgtc gattgcgcga gggcgtttgc tgcaggcggg attgagaccg
     1861 aacccagtgc tggattctga atacacttca gcgcgcattc tcggtggtca ggacgaggga
     1921 gatttttccg ttggggtgtc ccagcttttt gagactggag gcaaaagaag caagcgcgtg
     1981 accgtagcac gcctcgagct ggcccaggtt caggctgaag tgcgcgcatt ggagcgacaa
     2041 tttgccgccg atcttcgcgc ggcctatgcg cgggctctgg cggcggcgag gcagctcgat
     2101 acggaggaga aattaattgc tgcgaacgag gaaatcgtcc gagtcacgac ggcgcgtttg
     2161 aatgaaggag acgtggcgcc cctggacctg aatctcgttc gggtcgagac cgatcgcttg
     2221 cgagcgcaaa ccgtgcggac gcgagctgac ttggaaggtc aactgatctc gattcggacg
     2281 ttcgccggct tcgaggtaac tgagcctctg cgaattgctc cgctgccgga gaagccgccg
     2341 cgacttgatc tttcgcttcc cgaagctacc gacatggccc tacgcgaacg cgctgatttg
     2401 caggccgccc ggttaggcga agaggttggc agcgcgcgca ttcaactggc tcaggcccag
     2461 gccgttccga atttagcttt aacgtcgaga tactctatta gacgtgacat ttttgacgac
     2521 acgccggttg gaatcctgag ggacaaagat cgacttctta cttttggggt ttcggtagat
     2581 attccggtct ttaatcggaa tcagggagag atagtttcgg ccgtaggcga gaagacccag
     2641 gcccagcgac agagagagtt cctcgaggcg accattaagc gtgatgtggc gttggcgtat
     2701 cgacgttacc gcgcggcgac ggaagccctg gtgctctacg cgactcagat catacctatc
     2761 gcggagaaga acgtgcaggc tgtccgtgct gcctacaatg cgggggagtt ttcggtattc
     2821 gatgtgattg ccgagcaacg tcgcttgatt gagaacgaga ctgcctataa cgaatcgctt
     2881 ggggagtatt acacctcgct cgcagaactt gagcgcgctc ttgggatcac cttgccgccg
     2941 tcggcttttg cgccggggac gatcacggtt gttcccgaca ttgaccggct taccggtggc
     3001 aagctcgtgc agtcgatcaa atcgacgatg ttgatggacc aaccgattgc caacggaggc
     3061 aagttgccgc tgtccgggcc ggcggtcacg aactcacaga cggagaggat agttccacaa
     3121 aattagtcaa tttagaaacg ggaggcatcg ccatcatgaa aggactctta actctgcttc
     3181 tggccagttt acttgcaggc ttcgccgcgg catgtgcgac gtcagagaac actaataccg
     3241 cacctgcgac aagcaccgct aatacggcgc cgaagccggc tgcaaaggat gatgtgccac
     3301 caagcgtgca tgccgccttt cccgacgccc agtcgataac caaagatcac aaggatttga
     3361 ctacggccca gattgcggag attgaaaagg aggctggtag taagctgcct gatacggatc
     3421 accatgcata ccttgctttt tcgaccagcg gcggag
//
LOCUS       DASULZ010000482         4535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_667739, whole genome shotgun sequence.
ACCESSION   DASULZ010000482 DASULZ010000000
VERSION     DASULZ010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667739"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(602..1513)
                     /locus_tag="VFY96_09860"
     CDS             complement(602..1513)
                     /locus_tag="VFY96_09860"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HEX5606809.1"
                     /translation="MIDQECFQDHSHPILIMTSGGHKRLLPVVFWTLLACLSVAQSRA
                     GSAPPALLLANELRPEIDPVNYLVSEKYDGVRAVWDGKTLRFRSGRAVGAPQWFIAKL
                     PRESLDGELWLGRGRFESISGVVRKTEPEDEEWRQIKYMIFELPEAPGTFAERARRIK
                     EIVARVNWPQLIAAEQFQVADRAALKRKLDEVVRGGGEGLMLHLADAPYSTGRSDVLL
                     KLKPLQDTEAVVIEHIPGKGKYQGMMGALRVKTPAGKMLQIGTGFNDATRKNPPPIGT
                     MITYTYRGLTTTGLPRFPSYLRIREEF"
     gene            complement(1518..2894)
                     /gene="dnaB"
                     /locus_tag="VFY96_09865"
     CDS             complement(1518..2894)
                     /gene="dnaB"
                     /locus_tag="VFY96_09865"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX5606810.1"
                     /translation="MATLDDNLRKIPPQNLEAESSVLGGILLENEAINQVIEVLRAED
                     FYRESHRKIFRAMIEITDRNEPVDLITLSDFLKSRNELEAVGGSAYLAALADFVPTAA
                     NISYYARIVREKAILRSLISTATEIATRGYEEQGNVEEFLDSAEKVIFDISEKKIKAA
                     FIAVGDMITDTLKTIDKLYQRKEMVTGVPTGYEDLDKLTAGLQPADLIIIAGRPGMGK
                     TAFALNMAANAAYTGVGAAIFSLEMAKEQLVLRMLCSEARVNSSKVRSGYLGERDFPQ
                     LAKAAGRLHEAPIYIDDTPAISVLELRAKARRLLRDRSKKVGLIVVDYLQLMRGMGNA
                     NNREQEISEISRSLKALAKELNVPVIALSQLNRKVEDRTGRKPQMSDLRESGAIEQDA
                     DVIMFIYREEVYDKTNEDVKGKAEVIVEKQRNGPTGTITLTFIGEYTRFENYTERDDY
                     SFDEADEA"
     gene            complement(3148..3537)
                     /gene="queF"
                     /locus_tag="VFY96_09870"
     CDS             complement(3148..3537)
                     /gene="queF"
                     /locus_tag="VFY96_09870"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEX5606811.1"
                     /translation="MATAPSTELETFSNPRPERDYEIHMECPEFTCVCPRTGQPDFAT
                     IKIKYVPDKLCIELKALKLYLWSFRDQGTFHEAVVNKILDDLVAACRPRKMTVVGDFN
                     IRGGIHTTVTAAHPTSEVAPSKDAHNL"
     gene            3642..4436
                     /locus_tag="VFY96_09875"
     CDS             3642..4436
                     /locus_tag="VFY96_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606812.1"
                     /translation="MSTLTEPKAGEKLSAPQAKAYVDYLYSTLYKYAHEKIYKGAFMT
                     QLREGKLPLPVMRQFFKNWGHFSLEVNALNAVSYYTHLPFFVRHFDLLGPFCAKIADE
                     LISPRAPGHVLVLLQTAEKMGLNKREMLEEPYLPAARAINDFCHKIFIDGSIIELWGL
                     HVFEESLSMWSGQWFTALTRHYGFSKEDAIYFSTHEEADMDTHKLGDGGEEAMGHGAF
                     NRTVLTRALQESIEFRAGYSMEYCALTMVDLHAQMKQAALDNPYNP"
BASE COUNT         1005 a         1252 c         1215 g         1063 t
ORIGIN      
        1 cacaatttgg cacgctttcc ccaggtcttg ttcacttctg taacgggcgt gagattatga
       61 atgacttcgt ggagcgccgt atcgacactg acctgaagca caccgctccg cgttttctgt
      121 ttttaatgga ggagcaaaag ctcagcgctt tgattgggcg tgaaaccgag aaagaagcga
      181 caaccgtcga ctttagccgg attcgctgtc cactctgcaa gtggcaaccc aaaccggcac
      241 accgctggtt ttgcgcaagc tgcagttatc ctgaattttt tgatgcaggc tgcggaacgt
      301 gctggaacac tttcacgact cggggccgct gtccgggttg caaccatcaa tggcgctgga
      361 cggcgtgctt gaattgcgcg caatggtcgc tgcacgcgga ctggtacgaa accgattcgg
      421 gtaagcgaaa ttcaaaaagt ctgtaggcac ccgtaaagca gcatgctgcg ctcctacatt
      481 ttttccgtcc atcctgagta gcgcgtcctt cgatacggcg gctgtgccgc ctactcagga
      541 cgaccggcca ccccaagtag cgcgaagaga ggaaactcgt atcgcaggat tgcttgaagc
      601 cttagaattc ctctcgaatc ctgagataac tgggaaaccg tggcaagccc gttgtggtga
      661 gaccgcgata cgtgtaggtg atcatcgttc cgatcggcgg cggatttttt cgcgtcgcat
      721 cgttaaaccc ggtgccgatt tgaagcatct tccctgcggg agtctttacg cgcaaagcgc
      781 ccatcatccc ctgatatttt cctttgccgg gaatgtgctc gatcaccaca gcttcagtat
      841 cttgcaatgg cttgagctta agcagcacgt cactgcgtcc ggtcgaatag ggggcatcag
      901 ccagatgcag catgagccct tccccgcctc cgcgtacaac ctcatcgagc ttacgcttga
      961 gcgcggcgcg atctgccact tgaaactgct ccgcagcgat gagctgcggc cagttgactc
     1021 gggcaacaat ttccttgatt cggcgcgcgc gctcggcgaa cgtaccgggc gcttcgggca
     1081 gctcgaaaat catatatttg atctgccgcc actcctcatc ttcgggctcc gttttccgca
     1141 cgactcccga aatggactca aatcgcccgc gccccagcca gagctcgcca tcaagcgact
     1201 cgcgcggcag ttttgcgata aaccattgtg gagcaccgac cgctcgaccg ctccgaaagc
     1261 gcagcgtttt gccgtcccag acggcgcgca ccccgtcata tttttcactg accagataat
     1321 tgacgggatc gatttccggg cgcagttcat tggcgagcag caaagccggc ggcgcgctcc
     1381 cggccctgct ctgagcaacg ctcaagcagg caagtagcgt ccagaagacg acaggcagca
     1441 gtcgtttatg accgcctgag gtcatgatga gtatgggatg tgaatgatcc tgaaagcact
     1501 cctgatcaat caacgggcta agcctcgtcc gcttcatcga agctataatc gtcccgttcc
     1561 gtgtagtttt cgaagcgagt gtattctccg atgaaggtca acgtgatcgt gccggttggc
     1621 ccgttgcgct gtttctcgac gatgacttcc gctttgcctt tgacgtcctc gttcgttttg
     1681 tcatagacct cttcgcggta aatgaacatg atgacatcgg cgtcttgctc gatggcgccg
     1741 gattctctga ggtccgacat ctggggcttt ctgccggtgc gatcttccac tttgcgattc
     1801 agttgcgata acgcgatcac cgggacgttg agctccttgg cgagcgcctt caacgatcgt
     1861 gagatctcgg aaatttcttg ctcgcgattg ttggcgttcc ccatgccgcg catgagctgg
     1921 aggtaatcga ccacgatcag gccgactttc ttggagcgat cgcgcaagag ccggcgcgct
     1981 ttggcgcgca attcgagcac ggaaattgcc ggagtatcgt cgatatagat cggcgcttca
     2041 tggagccgac ccgcggcttt ggcgagttgc ggaaaatccc gctcgcccaa ataccccgag
     2101 cgtactttag agctattgac ccgtgcttcc gagcaaagca tgcgcaaaac cagttgctcc
     2161 ttggccattt ccagcgagaa aatcgccgcg ccgacgccgg tgtaagcggc gttagcggcc
     2221 atgttcagtg caaatgccgt ttttcccatg ccgggcctgc cggcaatgat gatgagatct
     2281 gccggttgca ggccggcggt gagcttgtcg aggtcttcat aaccggtcgg cacaccggtc
     2341 accatctctt tgcgctggta aagcttgtcg atggttttca gggtgtcggt gatcatgtcg
     2401 ccgacggcga taaacgcagc tttgatcttc ttctcggaga tgtcgaagat cactttttcc
     2461 gcgctatcga gaaattcttc gacgttcccc tgctcttcgt agccgcgcgt ggcgatctcg
     2521 gtggccgtgg agatcaggct tcgcaggatc gccttctcac ggacgatccg cgcgtagtag
     2581 gaaatgttgg ctgcggtggg aacaaaatcg gcgagcgccg ctaaataagc gctgccacca
     2641 acggcctcga gctcatttct gctttttaag aaatcactga gtgtgatcaa atccaccggc
     2701 tcgttgcggt cggtgatctc gatcatcgcg cgaaagattt tccgatgcga ttcgcggtag
     2761 aagtcttcgg cccgaagaac ctcgatcact tggttgatgg cttcgttctc gagcaaaatt
     2821 ccgcccagaa ccgacgactc tgcctctaaa ttttgcggcg gaattttgcg gagattatcg
     2881 tctaacgtag ccatttcaaa aatttccgtg accgagtgcc gcgagaaaac tgacgtgcgg
     2941 ctcagccgaa atttttagct aggcgaatgt aaagaattta aattaaaaag caagggaaaa
     3001 ttgaccgccg gtgaaaaaaa gttctcgcgg tcacaaaaaa acatcgctgc aaaaaaagat
     3061 ttttcgccaa gtgcatacgg ttttgcacag gcagcgaaca gcttatttac agcttattca
     3121 caaatcggct ggcaagagct gaacggatca aagattgtgc gcgtctttgc tcggcgcaac
     3181 ttcgcttgtt ggatgcgcag cggttaccgt cgtgtgaatt ccgccgcgga tattgaaatc
     3241 accgaccacc gtcattttac gcgggcgaca cgcggcgacc agatcgtcga gaatcttgtt
     3301 gacgacggct tcgtggaaag tgccttgatc gcgaaaagac cagagataga gtttcagcgc
     3361 cttcaactct atacagagtt tgtccggcac atatttgatt ttaatcgtgg cgaaatcggg
     3421 ctggccggtg cgcggacaca cacaggtgaa ctccggacat tccatatgga tctcataatc
     3481 gcgttccggc ctcggattcg aaaaagtttc cagttctgtg cttggtgcag tagccacacg
     3541 gccattctac ctcgccgcaa aaacgtttcc aaattgccca gttaacttga cttgggtggg
     3601 cctcgatgat agcccaaaaa cagcaatttc gggaggatga cgtgagcacg ctaacagaac
     3661 cgaaggcagg ggagaaactc agcgcgccgc aagccaaggc ttacgtcgat tacctctatt
     3721 ccacgctcta caaatacgca cacgaaaaga tctacaaggg cgccttcatg acgcagctcc
     3781 gcgagggcaa gctgccactc ccggtcatgc gccaattttt taaaaactgg ggacactttt
     3841 ccttggaggt caacgcgtta aacgccgtct cttactacac tcacctgcct tttttcgtgc
     3901 ggcacttcga cttgcttggg ccgttctgtg cgaagatcgc cgacgaattg atttcgccgc
     3961 gcgctcccgg gcacgtgttg gtgctgctgc aaaccgcgga gaagatgggt ctcaacaaga
     4021 gagagatgct cgaggaacct tatcttcccg ctgcgcgagc gattaacgac ttctgccaca
     4081 aaatctttat cgatggctcg atcatcgagc tgtggggact gcatgtgttc gaggaatcct
     4141 tgtccatgtg gtctggacaa tggttcacgg cattgacccg tcattacgga ttttccaaag
     4201 aggacgcgat ttatttttcc acgcacgaag aagcggatat ggacacgcac aaactcggcg
     4261 acggcggcga ggaagcgatg ggacatggcg catttaaccg tacggttctg actcgcgcgc
     4321 tccaggaaag catcgaattt cgtgccggct attccatgga atattgcgcg ctcacgatgg
     4381 tcgatctgca cgcacagatg aaacaggccg cgctggataa tccatacaat ccataactca
     4441 aaatccgatc ggccgcgcga gtcgatctat gcccgcaatc cagtcatgag tgacagcgaa
     4501 tttcattcct tgcactcgat tctcaaggac ttagc
//
LOCUS       DASULZ010000483         3053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1276387, whole genome shotgun sequence.
ACCESSION   DASULZ010000483 DASULZ010000000
VERSION     DASULZ010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276387"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VFY96_09880"
     CDS             <1..614
                     /locus_tag="VFY96_09880"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5606813.1"
                     /translation="RWIAKVTEKSRGRLRWVAMLPLLSIDKAVEEVRWAKDHGACGVF
                     KKGIECSDRTASDPYFFPVYDEASRLDLPVCIHNATGKVESAIGLQGEALDGGMNAIS
                     AFSALVEHGIPDLFPNLRVGFIETGASWIPYLYADLVAKKLRRTFLPVNFTEDLFRRN
                     RIYVACQTNDDLPYILKYGTEDNLMIGTDYSTQTSLPKSRRST"
     gene            complement(732..2093)
                     /locus_tag="VFY96_09885"
     CDS             complement(732..2093)
                     /locus_tag="VFY96_09885"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX5606814.1"
                     /translation="MATGFTEQLAIHTAKVRYEDIPPEAIEKAKDCILDQIGVELIGS
                     TLEWNKIAYRYVTEMGGRGESTIVDYGNKVPTLDAAFVNATFGQGCELDDVGFAAGGH
                     PGAATVPVALAVGEKQRSSGKDFLAAVVVGCDVMYRLLAAVRPSSGKRGFHSHGIGAP
                     FGAVASAARLLHLTDGQMLHAFGIAGMHSSGTMEYDQTGGEVKRVIAGIAARGGIQSA
                     LLAQMGLTGPPTIIEGKRGFLKVFADVVEPEEITRDLGREFKIMRIWFKLYPCVATVQ
                     GVIYTLSKLIDEHRFSADDVEEIRVGISETSLSHGGAIYEPRDTASAQFSLPFSLAIR
                     LLKNDNDLTFYMDPKLWTDPKVLALARKVKCYADPNAKQDQNYNTTMEVKLTNGKTHK
                     AFEPYPPGSPLNMVSRDVLRAKFRKMARAVLTEERIDQLIEAVDRLESYDDAAKLLPL
                     LVK"
     gene            complement(2397..>3053)
                     /locus_tag="VFY96_09890"
     CDS             complement(2397..>3053)
                     /locus_tag="VFY96_09890"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX5606815.1"
                     /translation="DVECAYEHRYSYPYGIMIDINGKRFVDEGEDFFSYTYAKCGREV
                     LRLPWRTAYQIFDSKTRPLLRSEYNRGFHVSADTIEALAKKLPGLDWENVVKTVGAFN
                     DAVNDAPFDPSKHDGKCTRGISPVKSNWAQRLDTPPFYAFPVTCGITFTFGGVGITEK
                     GEVKDTSGNVIGGVFAAGEITGGSFFNNYPGGAGLMKGAVFGRLAGSSAAAFAKSRRA
                     "
BASE COUNT          621 a          927 c          844 g          661 t
ORIGIN      
        1 atcgctggat cgcgaaagtt accgagaaaa gccgcggccg gctccgctgg gtcgccatgc
       61 tgccgctgct ctccatcgac aaggcggtcg aggaagtgcg ctgggcgaag gatcacggcg
      121 cttgcggcgt gttcaaaaag ggaatcgaat gcagcgaccg caccgccagc gatccgtact
      181 tctttccggt atacgacgag gcgagccgcc tcgatcttcc tgtctgcatc cataatgcga
      241 cgggcaaagt cgagtccgcc atcggcctgc aaggagaggc cttggacggc ggcatgaacg
      301 cgatctccgc gttcagtgct ctcgtcgagc atggcatccc ggatctgttt cccaatctcc
      361 gggtgggctt catcgaaacc ggcgcttcgt ggattccgta cctttatgcc gatctcgtag
      421 cgaagaaact tcgccgtacc tttttgccgg tcaatttcac tgaagattta tttcgccgca
      481 atcgcatata cgttgcctgc cagaccaacg acgatctgcc ttacatcctc aagtacggca
      541 cagaagacaa cttgatgatc ggcaccgatt acagcacgca gaccagtctg ccgaaatcca
      601 ggcgctcgac gtaattgagc agcgcagcga acgtggcgaa attcctgccg ccgtcgcgcg
      661 caaaatcctg gatgacaacc cgcgccgatt ttacgggctt taagatgact ctgtggattg
      721 cggacgctac gctacttcac aagcagcggg agcaatttgg ccgcatcgtc ataactttcc
      781 agccgatcca ccgcttcaat caactgatcg atgcgctctt ccgttagcac cgcgcgcgcc
      841 attttccgaa acttcgcccg caacacgtcg cggctcacca tattgagcgg cgagccgggc
      901 ggatagggct cgaatgcttt gtgagttttc ccattggtga gcttcacctc catcgtcgta
      961 ttgtaattct ggtcttgctt cgcgtttggg tccgcgtagc acttcacttt cctcgccagc
     1021 gcgagaactt tcggatcggt ccagagtttc ggatccatat agaaggtcag atcgttgtca
     1081 ttcttcaata ggcgaatagc caatgaaaac ggcaagctga actgcgcgct cgcggtatcg
     1141 cgcggctcat agatcgcgcc gccgtgcgaa agcgacgtct cggagattcc gacacgaatc
     1201 tcctcgacat catcggcgct gaagcggtgt tcatcgatga gtttgctcaa cgtgtagatg
     1261 actccctgca cggtggcgac gcagggatag agcttgaacc aaatgcgcat gatcttgaac
     1321 tcgcggccga ggtcgcgggt gatttcctct ggctccacca catctgcaaa gactttcaga
     1381 aatccacgct tgccttcgat gatcgtcggc ggaccggtca gccccatttg cgcgagcagc
     1441 gccgactgaa tgccgccgcg cgccgcaatc ccggcgatca ctcgcttgac ctcgccaccg
     1501 gtctgatcgt attccatcgt gccgctcgag tgcatgccgg cgatgccgaa cgcatgaagc
     1561 atttgcccat cggtcaagtg cagcaatctc gccgcgcttg cgactgctcc aaatggcgcg
     1621 ccgatcccgt ggctatgaaa accacgcttt ccggacgaag gacggacggc cgcgagcaag
     1681 cggtacatga catcacagcc gaccacgacc gcggcgagaa aatctttgcc actgctgcgt
     1741 tgcttttcac cgaccgcgag cgcgaccggg acggtagccg cgcccgggtg tccacccgcc
     1801 gcgaagccga catcgtcgag ctcgcaacct tggccaaagg tcgcattgac aaatgcggca
     1861 tcgagggtcg gcactttatt gccgtaatcg acgatggtgc tttcgccgcg tccgcccatc
     1921 tcggtgacgt agcgatacgc gatcttgttc cactccaaag tggaaccgat cagctcgacg
     1981 ccgatctggt cgagaatgca atctttcgcc ttttcgattg cttcaggcgg aatatcttcg
     2041 tagcggactt tagctgtgtg gatcgccagc tgttcggtaa agccggttgc cattctcttt
     2101 ctcctgttta actgtacgtc tgcgagaata ttaattgtcg ctcacgatgc acttcaccgc
     2161 actgtcgcag acaacaacca acatcctcct tgcaaaaatc attgcaccgt tcgccgaaac
     2221 ggcgtccttg acaggtcacc attgcgggct gctcaaaaca tctcagaggc gagtcgcgcg
     2281 aatggtcggc gagcgcaggc gtatcagccc gtacggtgac acgaagcgat tgagcgtacc
     2341 cttcgacgat gctcagggca atcggcgttg gcaatgagcc ttcttcagga gcccggttac
     2401 gcgcgcctcg acttggcgaa cgcagcagcg ctgctgcctg ccagacgacc gaatacagcg
     2461 cctttcatca agccggcccc gccgggataa ttgttgaaga acgagccgcc ggtgatttct
     2521 ccggcggcga aaactccgcc gatcacattc ccactagtat ccttgacttc gcctttttcg
     2581 gtgatgccaa cccctccgaa ggtgaacgtg atgccgcagg tcaccgggaa tgcgtaaaac
     2641 ggcggcgtgt ccaagcgctg cgcccagttc gactttaccg ggctgatgcc gcgcgtgcat
     2701 ttgccgtcgt gtttcgatgg atcgaacggc gcgtcgttga ccgcgtcgtt gaatgcgcca
     2761 acggttttaa cgacattttc ccaatccaag cccggcagtt ttttcgccag cgcttcgatt
     2821 gtatcggcgc tcacgtgaaa gccgcggttg tactcggagc gcagcagcgg gcgggtcttg
     2881 ctgtcgaaaa tctgataggc ggtccgccac ggcaggcgca acacttcgcg gccgcatttc
     2941 gcgtaggtgt agctgaaaaa atcctcgccc tcgtcgacga agcgcttacc gttgatgtcg
     3001 atcatgatgc cgtaggggta cgaatagcgg tgctcgtagg cgcactcgac gtc
//
LOCUS       DASULZ010000484         3337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_668031, whole genome shotgun sequence.
ACCESSION   DASULZ010000484 DASULZ010000000
VERSION     DASULZ010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668031"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="VFY96_09895"
     CDS             complement(<1..844)
                     /locus_tag="VFY96_09895"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606816.1"
                     /translation="MMKLRVLFFAFLCMATGFSAKFTLAAESTYKIRIGFPSLAFSYL
                     PFYVAQEKGIYKKYGIESEYIQMGTTIQPQAVVNGNINYFTSVSTGISAAVAGLPVVI
                     VISFCDSSPWVLVTNKDINKPQDLIGKTVALSGVRTSPYYFFQAFLKKSEISDKDVHI
                     INTGGTADSFRALTSNRVVATVMTPPFDDKAVSLGYKKFMQLGDLADIPYVGLVTSQN
                     EIKSNRESVRRTVAAVMDSLGWLRANRAESVKMIIDKFKVNQQEAENTYATLIRLLNK
                     DGELN"
     gene            complement(855..2057)
                     /locus_tag="VFY96_09900"
     CDS             complement(855..2057)
                     /locus_tag="VFY96_09900"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5606817.1"
                     /translation="MMENFDDLLVVDADGHVYEGNVDLASRLPEKWRSQAPIRLKDNE
                     GNSRILLEGRLWSASQGLGPGVSGPMTEKARGYRAGMVDPQARLKDMDEEGIDVAILF
                     GTQIALTVNGLMNKELAAVLCRAVNDWLIEYCSTNPQRLLAVGLIPCQDPPAAVKELE
                     HLKKAGAVTAMLPTNVYGLNNMGDRMFDPIYQCAQEIEMPLSVHPQTGHDGVLGVSGV
                     MGAGSERLRKYAYVHMTAFPFELMIAMMHMIGEGVFDRYSKLRVGFMEGGAGWLPFWA
                     ERFDEHIEKLAPQMPDLRRRPSDIIRGEQLTLTCESEESGLDRVFAANGESTVMYASD
                     YCHWDCHFPYSVKDIVDGKDLTFAQKEKLLGKNAVDFFWLKNLPKPAALRHARHLWNE
                     KPAKAAVA"
     gene            2201..>3337
                     /gene="cofG"
                     /locus_tag="VFY96_09905"
     CDS             2201..>3337
                     /gene="cofG"
                     /locus_tag="VFY96_09905"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF004884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HEX5606818.1"
                     /translation="MQALVMQQILQDSLRGKKLERDEALALSAAADDELRALLQTAAA
                     IRNQHKGRTVTFSPKIFVPLTNLCRDFCGYCTFRKAPDEPGAKTMTLDEVLRIVNQGK
                     RLGCTEVLFSLGDKPEAIYPEMKKFLSQRGHQRTLDYLHESCRSVLEQTGLLPHSNPG
                     VMGRGDLERLKQVNASMGLMVESVSERLLTAGAAHDNAPDKKPAIRLKTIAEAGRLRI
                     PFTTGILIGIGENWAERIDALLAIRDLHACYGHIQEVIVQNFRAKPTIPMHAHPDASR
                     DDMLKTIALARLIFGGEMNLQAPPNLTPNGYELYLEAGINDWGGVSPLTPDFINPEAP
                     WPALKMLSQKSAAAGFHLKARLPIYPEYIREEFLAPSVLRCIERL"
BASE COUNT          771 a          917 c          890 g          759 t
ORIGIN      
        1 gattgagctc tccgtctttg ttgagaagcc gaatcaaagt ggcgtaggta ttttccgctt
       61 cctgttgatt cactttgaat ttgtcgatga tcatcttcac gctttcggcg cggttggcgc
      121 gcaaccagcc gagggagtcc atcacggcgg cgaccgtgcg ccggaccgac tcgcgattgc
      181 tcttgatctc attttgagaa gttaccaagc cgacataagg aatgtcggca agatcgccga
      241 gctgcataaa tttcttataa ccgagggaga ccgctttatc atcgaacggc ggcgtcatta
      301 cggttgcgac cacgcggttc gacgtcaggg cacgaaagct gtccgcggta ccgcccgtat
      361 tgataatgtg cacgtctttg tcactgatct cggacttctt caaaaaagcc tgaaaaaaat
      421 agtagggcga tgtgcgcacg ccggagagcg ccaccgtctt gccgatcaga tcttgcggtt
      481 tgttgatatc cttgtttgtg accaacaccc agggagagct gtcgcaaaag ctaatcacga
      541 tcaccacggg tagtccggcc acggcggcag aaatcccggt ggagacggaa gtaaagtagt
      601 tgatattccc attgaccacc gcttgcggtt gaatcgtcgt gcccatctgg atgtattccg
      661 actcgatgcc gtattttttg taaatgcctt tttcctgcgc cacatagaac ggcaggtagg
      721 aaaacgccag ggacggaaag ccgattctaa ttttgtacgt gctttccgct gccagcgtga
      781 atttggcgct gaaacctgtc gccatacaca aaaaggcgaa aaataaaacg cgtagcttca
      841 tcatcacgag atcctcaggc aacggcggct ttcgccggct tctcattcca taaatggcgg
      901 gcatgcctta gggcggcggg cttgggaagg ttcttgagcc agaaaaagtc gacggcgttt
      961 ttgccgagca gtttttcttt ctgcgcgaat gtcaaatcct tgccgtcgac gatgtctttt
     1021 accgagtaag ggaagtgaca gtcccagtgg cagtagtccg acgcgtacat gaccgtgctc
     1081 tcgccgttgg cggcaaaaac acggtccaga ccgctttctt ccgattcgca cgtcagggtc
     1141 agttgctcgc cgcgaatgat atcactcgga cgccttctaa gatcgggcat ctgcggcgcg
     1201 agtttttcga tatgctcgtc gaaacgctcc gcccagaaag gcaaccagcc tgcgccacct
     1261 tccataaagc cgacgcgaag tttcgagtag cgatcgaaca cgccttcacc aatcatgtgc
     1321 atcatggcaa tcatcagctc gaagggaaat gccgtcatgt gcacataagc atatttgcgg
     1381 agtcgttcgc tgcccgcccc cattacgccg gaaacaccca gcacgccatc atggccggtt
     1441 tgcgggtgca cgctcagcgg catctcaatc tcctgggcgc attggtaaat cggatcgaac
     1501 atgcggtcac ccatgttgtt caagccatag acgttggtcg gcagcatcgc cgtcaccgcg
     1561 ccggctttct ttagatgctc aagctctttt actgcggctg gcggatcctg gcagggaatc
     1621 aaaccgactg cgagcaaccg ctgcgggttt gtggaacagt attcgatcag ccaatcgttc
     1681 accgcgcgac agagcaccgc cgcgagctcc ttattcatca aaccgttgac ggtaagcgcg
     1741 atttgagtgc cgaagagaat ggcaacgtcg atgccttcct cgtccatgtc tttcagccgc
     1801 gcctgcggat ccaccatgcc ggcgcgataa ccgcgcgctt tttcggtcat cggacccgat
     1861 acacccgggc cgagcccttg ggaagccgac cagagtcgcc cctccaaaag aatgcgcgaa
     1921 ttgccttcat tgtctttcaa ccgaatcggc gcctgagaac gccatttttc gggcaaacga
     1981 gaggccaagt cgacattgcc ttcataaaca tgaccatcag cgtcgaccac cagtagatcg
     2041 tcaaaatttt ccatcatttt cctccgaatc gagtcgcatg cttgagcgga atctcatcga
     2101 accactcgga ctaagacgta ccatagcaaa agtatttcgg gcaaccgcaa tttgagctgc
     2161 ttgaaactta tccggtttga aagccagggc tcgattcgat atgcaagcat tagtgatgca
     2221 gcagattttg caagacagtt tgcgcggaaa aaaattggag cgggatgaag cgctggcgct
     2281 tagcgcggca gcagatgatg agcttcgagc tctgctccag accgcagccg cgatacgtaa
     2341 tcagcacaaa gggcgcacgg tcactttttc tccgaaaatt tttgtgccgc tgaccaatct
     2401 gtgccgcgac ttttgcggct actgcacgtt tcgaaaagcc ccggatgaac ccggcgcaaa
     2461 gaccatgaca cttgacgagg ttttacggat cgtcaatcaa ggcaagcgcc tcggttgcac
     2521 ggaagtgctc ttcagcctcg gcgacaagcc ggaagcgatt tatcccgaga tgaagaaatt
     2581 tctctcgcag cgcggccatc aaaggacgtt ggattatttg cacgaatcct gccgcagtgt
     2641 cttggagcaa acgggtttgt tgccgcattc gaatccgggc gtcatgggca ggggagatct
     2701 cgagcgcttg aagcaggtca atgccagcat gggattgatg gtcgaaagcg taagcgagcg
     2761 gctgctcacg gcgggtgccg cccatgacaa tgcaccggat aaaaaacctg caattcgcct
     2821 aaaaacaatt gcagaagctg gaaggcttcg gattccgttc acgacgggaa tcctgatcgg
     2881 tatcggcgaa aactgggcgg aacggatcga tgccttgctc gcaattcgcg atctccatgc
     2941 gtgctacggc catattcagg aagtgatcgt gcaaaatttt cgcgccaaac cgaccattcc
     3001 catgcatgct catcctgacg ccagtcgcga tgatatgctt aaaacgatcg cgttggcgcg
     3061 gttgatcttt ggcggtgaga tgaatctcca ggcgccgccc aatttaacac cgaatggcta
     3121 tgagctctat ttagaggccg gcatcaacga ttggggtggt gtttcgccgc tcacgcccga
     3181 ttttatcaat ccggaagcgc cctggccggc gttaaagatg ttgagtcaga aaagcgccgc
     3241 agccggcttc cacttaaagg cgcgattacc catttatcct gagtacattc gagaggaatt
     3301 tctcgcccca tcggtcctcc gttgcatcga gcgcctc
//
LOCUS       DASULZ010000485         3879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1479336, whole genome shotgun sequence.
ACCESSION   DASULZ010000485 DASULZ010000000
VERSION     DASULZ010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479336"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VFY96_09910"
     CDS             complement(<1..436)
                     /locus_tag="VFY96_09910"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEX5606819.1"
                     /translation="MNKIKVAILDDFEKLADTVPAYDKLKVRADVTILRKRLDSSENI
                     VAALRDFDAVLLMRERTFLSDKEYAQLPRLKFISQTGRTSKHLDLANATRRHIAVTGT
                     PAENGMTTTELTVGLILSLLRKIPQVNSRMREEAWPAIAGHTL"
     gene            complement(444..1124)
                     /locus_tag="VFY96_09915"
     CDS             complement(444..1124)
                     /locus_tag="VFY96_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606820.1"
                     /translation="MQTRIFTWLGREFVELTGEAKPAVNAAAEAQEIFQRFESELKQH
                     GLSLANTVRSRLWGRDRQSRDLGSTERVKILSGKARSASSSYIAPGHFDSNAKVALDL
                     LAMRPANPNAEKRVVEYEPAIVPIRFLVYDSVVILSGVTTVLPTLAEQFDNIFPRIAG
                     SLKDAGSSWEKVARVSFYLHRSQTLENLKSLFARHVSAHVPQLEYCFVDGYSSEGKFC
                     EAEVTAMA"
     gene            complement(1356..2915)
                     /locus_tag="VFY96_09920"
     CDS             complement(1356..2915)
                     /locus_tag="VFY96_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5606821.1"
                     /translation="MQQRVLIRWFVLSLCTILPSSVYAQDAIRDLVVKIHAIHHSPDL
                     LRPWTKSAPRQVKGSGVVISGKRILTNAHVVKYASQIYVQPNQSASYIPARVEAMTPG
                     MDLAVLKLDDDAFFENRGVLPFAQELPRVKDAINVYGYPTGGTELSVTQGIVSRIEYT
                     DYYYQATGLRIQVDAALNFGNSGGPAVSDGKLVGLVFSLIQNAQNIGYLIPVEEIQLF
                     LNDIADGVYDGKPQFYDITQTVENDALRQRLGLPKGINGVMVAQPYRDSAEYPLQEWD
                     VITKIGDMPIDSDGKVPIRYDLRLSASYLVQKYAKNGIVSLTVYRDGRLIDVNLPVLP
                     RRELVIPYLMDNNPRFFIFGPFVFAPTTQDYLERLGNQQPPTLGHRPSPLVTRRYDKP
                     AFDKEELVVVAAPLFPSRITKGYDDPNRSVVSEVNGTPVKNLRHLVEILRDSQDERIT
                     FKFASSGVLTHETMVFNRKDLIEATGKILEENGIRYPYSPDLRTLWEASNRIGPQSAQ
                     ATCCVPIPTAN"
     gene            3097..3405
                     /locus_tag="VFY96_09925"
     CDS             3097..3405
                     /locus_tag="VFY96_09925"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX5606822.1"
                     /translation="MWYLVLRRPMKPRDQWTVSLDQHLIWMKQQHHAGKILFSGPTTD
                     RKYGVYVIRAESKQEAEQIAASDPYTAAGFCAFEVLEWEVHQIMGVGPFTSAEMRAHH
                     "
BASE COUNT          935 a          989 c         1104 g          851 t
ORIGIN      
        1 cgagcgtatg gccggcgatt gctggccaag cttcctcgcg catgcgcgaa ttcacctggg
       61 gaattttgcg caacagcgag aggatcaatc ccacggtcaa ttcggtcgtg gtcatgccgt
      121 tttccgcggg ggtgccggtg acggcgatat gccggcgggt ggcattcgcc aaatcgaggt
      181 gcttactggt acgcccggtt tgagaaatga actttaacct aggcaattga gcatattcct
      241 tgtcgctcag gaaagttcgt tcgcgcatca gcaggactgc gtcgaaatcg cgcagcgcgg
      301 cgacgatatt ttccgagctg tccaaccgct tgcgcaggat cgtgacgtcg gcgcgcacct
      361 tgagcttgtc gtacgccgga acggtgtcgg caagcttttc gaaatcgtcc agtatcgcga
      421 ctttgatttt gttcatacca tgattatgcc atagccgtca cttcggcttc gcagaattta
      481 ccttcggaag aatagccatc gacgaagcag tattcgagtt gcggaacatg cgcgctcacg
      541 tggcgcgcga aaagggattt caggttttcc aacgtctggc tgcggtgcag gtaaaacgac
      601 acccgcgcaa ctttttccca ggagctgccg gcgtctttta gcgagccggc gatgcgcgga
      661 aagatattgt cgaattgctc ggccaaggtc ggcaggaccg tggtcacgcc ggaaaggatg
      721 accaccgaat cgtaaaccag aaaacggatc ggcacgatcg ccggctcata ttcgacgacg
      781 cgcttttcgg cattgggatt cgcgggacgc atggccagca ggtccaacgc gactttggcg
      841 ttggaatcga aatgacccgg cgcgatgtaa ctcgagctgg cggaccgcgc cttgccggag
      901 agaattttca cacgctcggt gctgccgagg tcacggcttt ggcggtcgcg cccccagagc
      961 cggctccgca ccgtgttagc gagggacaag ccgtgctgct tcagctcgct ctcgaaacgt
     1021 tggaagatct cctgagcttc cgctgcggca tttaccgccg gttttgcttc acccgtaagc
     1081 tcaacgaact ctctgcccag ccaagtgaaa attctcgtct gcataggacc tctcctttga
     1141 cggcggcgat cctatatgaa ggcgttgttt cgatcaacga agagtttgca gggagtacag
     1201 cagatcaagt tttgcgagag aacaggaacc aatactctcg aaccactttg atcaacggct
     1261 cgccgaggaa aaactcggag gcgaaactga ggagaccaaa aacgatcaca agcaagagca
     1321 agaaaaaaaa cagcgcacct attttcgaca ttgcttcaat tcgcggtcgg gatgggcacg
     1381 cagcaggttg cctgagcgga ttgcggtccg attcgattgg atgcttccca gagagtgcgc
     1441 aaatcgggag aataggggta acgaatgcca ttttcttcga ggatcttgcc ggtggcttcg
     1501 atcagatcct tgcgattaaa gaccatggtc tcgtgcgtta gaacacccga gctggcaaat
     1561 ttgaacgtaa tccgctcatc ctgactgtcc cgcaagatct cgaccagatg gcggagattt
     1621 tttaccggag ttccattgac ttcgctcacc actgaacggt tggggtcgtc atatcctttg
     1681 gtgatccggc tagggaagag cggtgcagcg acgaccacaa gctcttcctt atcgaaggcg
     1741 ggcttatcat aacgtcgtgt gaccagagga ctcgggcgat gcccgagcgt cggtggttgt
     1801 tgatttccga gccgctccaa atagtcttgg gttgttggcg caaatacgaa cgggccgaaa
     1861 ataaaaaacc ggggattgtt atccatcaaa taggggatca ccaactcgcg tcgaggcaac
     1921 acgggcaaat taacatcaat gagccggcca tcccgataaa ccgtcagcga tacgatgccg
     1981 tttttggcgt atttttggac caggtaagaa gccgaaagcc gaagatcata acggatcggc
     2041 actttgccgt cgctgtcgat cggcatatca ccgattttgg tgataacatc ccattcctga
     2101 agcgggtatt ccgcgctgtc gcgatagggc tgcgcaacca tgaccccatt gatgcctttc
     2161 ggtaaaccga gcctttggcg cagagcatca ttttcgacgg tctgggtgat gtcgtagaac
     2221 tgcggtttgc cgtcgtagac accgtccgcg atgtcgttca gaaagagctg aatttcttcc
     2281 accggaatga gatagccgat attctgcgca ttctggatta agctgaacac aaggccgacc
     2341 aatttcccat cggatacggc gggcccgccg ctgttgccga agttgagagc ggcatcgacc
     2401 tgaatacgca gtccggtcgc ctgataataa taatccgtgt actcgatgcg cgagacgatc
     2461 ccctgagtga ccgaaagttc ggtacccccg gtgggatagc catagacgtt gatggcgtcc
     2521 ttcacacgcg gcagctcttg agcaaagggg agcacgccgc gattttcaaa gaacgcgtca
     2581 tcatcgagct tcaacacggc gagatccatt ccgggagtca tggcttcaac gcgagccggt
     2641 atgtaactag cggactggtt cggctgtacg tagatttgac tggcgtactt gaccacatga
     2701 gcattggtga ggatccgttt gccgctaatg acaacgcccg agcctttgac ctggcgcgga
     2761 gcgcttttgg tccatggccg caaaagatcc gggctgtggt gaatcgcatg aatctttacc
     2821 acgaggtcgc gaattgcgtc ttgcgcgtaa acggatgacg gcaaaatcgt gcacaaagat
     2881 agaacaaacc aacgaatcag cactcgttgt tgcatgatat ctcctcgatc gatatgtagc
     2941 gggaaagaaa acaggccgta ttatatacga aaaggcggcg aagtgaaggg aaaagaaact
     3001 gccggccact aaaaatttgc ggactacatc tgattgtgag tgcatcagca gaaattcgct
     3061 tggagcgaca atacgcctga aacaaaagga ggtcgtatgt ggtatttagt tttgcgccgg
     3121 ccaatgaagc cgcgagacca atggacggtt agtcttgatc agcacctgat ctggatgaag
     3181 cagcagcatc atgccgggaa aattttgttt tccggaccca ccaccgatag aaaatatgga
     3241 gtctacgtca ttcgtgcaga gtcgaagcaa gaagcagaac agattgccgc cagtgatccg
     3301 tataccgcgg cgggattttg cgccttcgaa gtgcttgaat gggaagtgca tcagatcatg
     3361 ggagtcggac catttacgtc tgctgaaatg cgtgcccatc attgacaaaa aaaaatttta
     3421 aaaaaacttg acacgagtcg tgccgggagt tagggaagag ttagccccat cctgctaggg
     3481 ggtgcgaaga gcgcgaacag agcgcgcctg ttgcggctct tcttgaatcg gagggattta
     3541 tgctgggaga agatagtcca ccgggccaat cacgcccgct aacggcgggc accggcagtt
     3601 cggttaagct gcacgtcgag catcgagctg cgtgaatcaa gccctcaaat tctgcgccac
     3661 atggcgtgaa cccgaaaatc gggtcaatca gaaggttggg cttcctcaaa gcagcgccga
     3721 gttcttcatt gacggtagcc gaaccgagcc agcccgagtg aatcgtgcgc acgcgggcag
     3781 cggttgttaa aagcttaatc agagataacg acgcgatggc gtcaacaaaa tgggcggtgt
     3841 gtctaggata cactgcccgt tttgttatga tgagtccca
//
LOCUS       DASULZ010000486         3848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_566923, whole genome shotgun sequence.
ACCESSION   DASULZ010000486 DASULZ010000000
VERSION     DASULZ010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566923"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1260
                     /locus_tag="VFY96_09930"
     CDS             <1..1260
                     /locus_tag="VFY96_09930"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5606823.1"
                     /translation="KIPYANVEAYGVYTNTVPAGAFRGFGALQVTWAYESQMDMIADR
                     LGIDPLEFRLKNLLRKGDLYTAGDTPVDCDMKEGLLKVADAIKWAEKPTERNRGKGIS
                     CCIKDAGGTYKVAGATVKMSSDGSVMLLTGTVEIGQGPRTALSQVVAEELSLPMDQIG
                     VAQLDTDVTPYDISTSASSSMVVMGTAVLRAAQDVRKQLLQCASKLLQQKVEHLKLHN
                     GQVVYGEGPPISYSKVIVDYFGSKAGEIIGKGLYKDKRNPKAVLGSTTTFWEVGWGGV
                     EVEVDPDTGVVHLLNYVSACDAGKAINPEQCIGQDEGAVLFGVGHTLIEEMIYEDGQL
                     LNGNLVDYSVPRFGDVPDKLETILIENRNGPGPFGSKGIGEGGLLPVASAVANAISRA
                     VGVRIQELPLIPPKVWRALQAKSDIKV"
     gene            complement(1443..1946)
                     /gene="nbaC"
                     /locus_tag="VFY96_09935"
     CDS             complement(1443..1946)
                     /gene="nbaC"
                     /locus_tag="VFY96_09935"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HEX5606824.1"
                     /translation="MFPHLQGFNLRKWIDENRTDWGQRRVIWRDSDFIAFVTRGPNRR
                     KDFHINPGDEIFYQLEGELNLHYLKEHKQTIAVLQRGDIFLLPKNTPHSPRRGEGSWT
                     YVVERQRGKDEVDRFIWPCENCGHQLYETEVRFDDPGDAVAKATAALKSDPSLAKCTE
                     CGVVLEL"
     gene            2013..2510
                     /locus_tag="VFY96_09940"
     CDS             2013..2510
                     /locus_tag="VFY96_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020481907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX5606825.1"
                     /translation="MLSVREKRLTLKTGRVGVISDTHGLVRPEAIDALKGSELILHAG
                     DIGKPEVLTALATIAPVIAIRGNNDRGAWAKKLPDILRLRVNGFAVYIIHNVNELEVD
                     PAAEGFRAVISGHSHKPSITNLDGVMFLNPGSAGPRRFKLPVVVARLAVQKPKIHAAI
                     IELEI"
     gene            2764..3309
                     /gene="pyrR"
                     /locus_tag="VFY96_09945"
     CDS             2764..3309
                     /gene="pyrR"
                     /locus_tag="VFY96_09945"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016821529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX5606826.1"
                     /translation="MAEEKKVLLERTELAKAITRMAHEIVEKCVAPSDLVLIGIRSRG
                     VHLARRLGRKVNEICQITPPVGIMDVTPYRDDRSRPENASALGALEATVAVDDKTVVL
                     IDDVIFRGRTIRAAMDAVERFGQPKRILVAALVDRGSRELPIRADVVGKNITATETQR
                     VNVMLEESDGLDQVTLAGWKT"
     gene            3318..>3848
                     /locus_tag="VFY96_09950"
     CDS             3318..>3848
                     /locus_tag="VFY96_09950"
                     /inference="COORDINATES: protein motif:HMM:NF014749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEX5606827.1"
                     /translation="MRHLISIADLTNDEIEEIFALADHAQELRKETPATGQIMATLFY
                     EPSTRTRLSFESAMQRLGGSVISCSDMKSSSTAKGETIADTAKVVSSYADVLVVRHHW
                     DGAVQAMSEHADAPVINAGDGAHEHPTQTLCDLYTLRKEKGGLKGLTVVVCGDLKNGR
                     TIHSLVFALARFGASVV"
BASE COUNT          867 a          998 c         1131 g          852 t
ORIGIN      
        1 aaaattcctt atgccaacgt cgaggcttat ggcgtttata cgaataccgt gcctgcgggt
       61 gcgtttcgtg gttttggcgc gctgcaggtg acttgggcat atgaatcgca gatggacatg
      121 atcgccgatc ggctcggcat cgatccacta gaatttcgcc tcaagaactt gcttcgcaag
      181 ggtgatcttt acacggcggg cgatacaccc gtggattgcg atatgaagga aggtttgctg
      241 aaggtcgccg atgcgatcaa atgggcagaa aagccaacgg agcgcaatcg gggaaaaggc
      301 atcagctgct gtattaaaga tgccggcggg acttacaaag tcgctggggc gacggtcaag
      361 atgtcgtcgg atgggagtgt gatgctgctc acgggtacag tggagatcgg acaaggcccg
      421 cgcaccgcgc tcagccaagt cgttgcggag gagctttcac tgccgatgga ccaaattggc
      481 gtggcgcagc tcgataccga tgttactccc tacgatatct caacgagcgc gagcagctcg
      541 atggtcgtga tgggaacggc agtcctgcgc gccgcgcagg atgtccgcaa gcaactgctg
      601 cagtgcgcat cgaagctgct gcagcaaaaa gtcgaacatt tgaagctcca caatggtcaa
      661 gtggtttatg gcgaaggccc accgatttcc tacagtaaag tgatcgttga ctatttcggc
      721 agcaaggccg gtgaaattat cgggaaaggt ctttacaaag ataaacgcaa tcccaaggcg
      781 gtgttaggat ccacaacgac cttttgggaa gtcggttggg gtggtgtcga agtcgaagtc
      841 gatccggaca caggtgtggt gcatctgctg aactacgtat ccgcctgtga tgccggcaaa
      901 gcgatcaatc ccgagcaatg tatcggccaa gacgagggcg cagttctgtt cggcgtcggt
      961 cacacgctca tcgaagagat gatctatgaa gacggacaat tactcaacgg caacctggtg
     1021 gattacagcg ttcctaggtt tggcgacgtg cccgacaaac tggaaactat tctgatagag
     1081 aaccgtaacg gcccgggtcc attcggctcc aaaggcatcg gcgaaggcgg tcttctgccg
     1141 gtcgcatcgg cggtcgccaa cgccatatcg cgtgcagtcg gtgtgcgcat tcaagagctg
     1201 ccgctcattc cgccgaaggt ctggcgcgcg ctgcaggcaa aatccgatat taaagtttag
     1261 cttgcgatta gctcgatcgt tacttgagga tggatgcgcg ccgggcgtcg atattgccgg
     1321 attacccgtg ctcaacagac cagggcgtcg gggatccgtc cgtcattccg gcgcgtaccc
     1381 gaatccagtt ttctttttga gaacaattct ggttgccggc atgacaaatt tgcattttga
     1441 tattacaact caagaaccac cccgcactcc gtgcacttcg ccagacttgg atcgcttttg
     1501 agagcggctg tcgctttggc taccgcatcg ccaggatcat cgaagcgaac ttcggtctcg
     1561 taaagctgat ggccgcagtt ttcacagggc cagataaacc tgtcgacttc gtccttgcca
     1621 cgctgccgtt ccacgacata agtccaagag ccctcgccac gccgcggcga atgcggagta
     1681 ttcttgggca ggagaaaaat atcgccgcgc tggagcacgg cgatagtctg cttatgctct
     1741 ttaagataat ggaggttgag ctcaccctcg agctgataaa agatttcgtc gcccggattg
     1801 atgtggaaat ccttgcggcg attggggccg cgcgtcacaa aggcgataaa gtctgaatcg
     1861 cgccagatga ctcggcgttg accccagtcg gtgcggtttt catcgatcca tttccgcaaa
     1921 ttaaatcctt gtaaatgtgg aaacatagag cctccgtttc atgaccgcaa tatagaattg
     1981 cgcgcgcaag cgaatcaagc taaatcgatc aagtgttatc ggtacgtgaa aaacgcctga
     2041 cgctgaaaac cgggcgcgtc ggtgtgatct cggacacgca cgggttagtc cgtcccgagg
     2101 ccatcgacgc gctgaaaggg tcggagctga tcctccatgc cggcgacatc ggtaaacccg
     2161 aggttctcac agcgttggca actatcgctc cggtgattgc catccggggt aataatgacc
     2221 gcggggcgtg ggcaaaaaag ctgcccgaca ttctaaggct ccgagtgaac ggtttcgccg
     2281 tttacattat tcacaacgtc aacgagctgg aagtcgaccc tgcggcggag ggctttcgag
     2341 cggtaatcag cggtcactcg cataagccaa gtattacaaa tctggacggc gtgatgtttc
     2401 ttaatcccgg cagcgccggg ccgcggcggt tcaagttgcc tgtggtcgtg gctcggctcg
     2461 ccgttcagaa accaaaaatc catgcagcaa tcatcgaatt ggaaatttga cgggtcggct
     2521 gaagtttgcc gccagcgata cgattcggaa tgcttcattg ttttccgctg ggacaccagc
     2581 aaacctgcgt ctcggagcct tgggcgaaag actttttctt gactttcctg cacccgcgcg
     2641 atatcaacaa gcaatgacct tttaattcga tcctgtgaga tcgaaaaagg gcaatatgat
     2701 cgaagtcgtt ctacagatag tttctgcaca aacgccctct cgattgctcg ggggggtttt
     2761 tttatggccg aagaaaaaaa agttctgctt gagcggaccg aactagccaa ggccatcacg
     2821 cgcatggctc acgagattgt cgagaaatgt gtcgcgccga gcgatctggt tttgatcggg
     2881 attcgctcgc gcggcgtcca tttggcgcgg cgcctgggtc gcaaggtcaa tgagatttgt
     2941 cagattacgc ccccggtcgg cattatggat gtgactcctt atcgtgatga ccggagccgg
     3001 ccggaaaacg cttctgcgct cggcgcactg gaagcgaccg tagccgttga cgacaaaacc
     3061 gtggtcttga tcgacgacgt gatttttcgc ggtcgcacga ttcgcgctgc gatggatgcc
     3121 gtggagcggt tcggccagcc taaacgtatc ctggtggcag cacttgtcga ccgcgggtcg
     3181 cgcgagctgc cgattcgagc ggacgtcgtc ggcaaaaata tcaccgcgac ggaaacccag
     3241 agggttaatg tgatgcttga ggaatcggac gggctcgatc aagtcacatt ggcggggtgg
     3301 aaaacctagg gcgatccatg cggcatctca tctccatcgc cgatttgacc aatgacgaga
     3361 tcgaggagat cttcgctctc gctgatcatg cacaggagtt gcgcaaagag acacccgcaa
     3421 ccggacagat tatggcgaca cttttttatg agccgagcac acgcacgcgg ctttcgttcg
     3481 agtcggcgat gcagcggctg ggcggctcgg tgatcagctg ttccgatatg aaatcttcct
     3541 ccacggccaa gggagaaacc atcgccgata ctgcgaaagt cgtttccagc tatgccgacg
     3601 tcctggtggt gcggcatcat tgggatggag cggtgcaggc gatgtcggag cacgcggatg
     3661 cgccggtgat caatgcgggc gatggcgccc atgagcatcc gacacagact ttgtgcgatc
     3721 tctacacttt gcgcaaagaa aaaggcgggc tcaaagggct cacggtggtg gtatgcggtg
     3781 atcttaaaaa tggccgcacg atccattctc tggtgtttgc tttggcccgt ttcggcgcca
     3841 gtgtcgtt
//
LOCUS       DASULZ010000487         4314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1073274, whole genome shotgun sequence.
ACCESSION   DASULZ010000487 DASULZ010000000
VERSION     DASULZ010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073274"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VFY96_09955"
     CDS             <1..213
                     /locus_tag="VFY96_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606828.1"
                     /translation="TQFLSDRLKGAGGFPGLTVQQARHVHIECAGARIHIDDDLWPKD
                     SSPAGDSKFAIDIGVQPQSLEILLPA"
     gene            362..1210
                     /locus_tag="VFY96_09960"
     CDS             362..1210
                     /locus_tag="VFY96_09960"
                     /inference="COORDINATES: protein motif:HMM:NF021996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HEX5606829.1"
                     /translation="MNKRNTIIGAAALVLAIAWYAFRPELLFIDRTVNEEFPGGAAMA
                     SIEKGSMELTKGTFKSLAHETKGLASIYQLSDGKRTLRLSQFETSNGPDVHVYLTAAE
                     VTKGSEAIKEAGFIDLGSIKGNKGDQNYDIPVNADLNKYKNVTIWCARFSVNFGEAAL
                     DAPASIPVKVAEGNFRGVAHQTRGMASIYKLPEGKNVLRFSAFETSNGPDVHVYLVAA
                     RDATDDEAVKKAGFLDLGSIKGNIGEQNYDLPIDIDLNKYRAVTVWCKRFGVNFATAP
                     LIPTKS"
     gene            1460..3031
                     /locus_tag="VFY96_09965"
     CDS             1460..3031
                     /locus_tag="VFY96_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015206746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX5606830.1"
                     /translation="MAANDHYDVVIIGSGAGGGTLAHKLAATGKKILILERGGYVPRE
                     KDNWNARAVNLEGKYQTKEKWHDASGNELHPHTNYYVGGNTKFFGAALFRLRKEDFGE
                     IKHHGGVSPAWPLTYDELEPYYTEAEKLYHVHGNAGEDPTEPRRSAPYPQKAVSHEPR
                     LQQLSDDFERLGLHPFHTPLGVMLDEKLPHLSKCIRCGTCDGFPCLLSAKSDAQVCAV
                     DPALQNPNVALLTDTYVDKLATDASGRRISKVVVRRQGQRAEFSADIVVVSCGAINSA
                     ALLLRSANDKHPRGLANSSDTVGRHYMGHVNSVLMALSKCPNPTIFQKSLSLNDFYFG
                     ASGWNYPMGHISFVGKLDGDTLRGGAPAIAPGWTLDLMAKHSLDFWLTSEDLPDPNNR
                     VTVDSEGGVVLSYSPNNQEGHKRLIVKLKELMQQQSKCSVHGHNCHEGLFARNLYIGQ
                     RIPLAGVAHQVGTARFGRDPRNSVLDTNCKAHDLDNLYVVDGSFFVSSGAVNPALTIM
                     ANALRVGEHLTERLK"
     gene            3176..4285
                     /locus_tag="VFY96_09970"
     CDS             3176..4285
                     /locus_tag="VFY96_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015155606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5606831.1"
                     /translation="MKIRKVWISAFAGMTKQKTRHPVDSNNISRLRAQASLIFRVLFL
                     VFSCLVFPFPAVGQLIKSVETIGMTVSNMDGASQFFSNVLRFEKISDFEVYGADYEKL
                     QGLFGLRMRVVRMKLGEETLELTQYLAPEGRPIPADWRSNDRAFQHIAIVVADMDKAY
                     QHLRAHNVRHASTAPQLIPAANPAAAGIRAFYFKDPDGHYLELIHFPQGKGDPRWQQK
                     SDKLFLGIDHTAVVVSSTENSLRYYRDLLGMRLAGESMNSGTEQEHLNNVAGARLRIS
                     GLRAISGPGIEFLEYLSPRNGRPAPKDTRANDIWHWQTMLRTGNAEKTFRKLNRGSGR
                     AVSSGIAEISEADFGFTKGFLARDPDGHGLQIVEK"
BASE COUNT         1036 a         1185 c         1142 g          951 t
ORIGIN      
        1 acgcaatttt tatcggatcg cttgaaaggc gcgggcggtt ttccaggact gaccgtgcaa
       61 caagcccgtc acgttcacat cgaatgtgcg ggcgcgcgca tccacattga cgacgatctc
      121 tggccaaagg atagttctcc tgcaggcgac tctaaattcg caatcgatat aggcgtccaa
      181 ccacaatctc tggaaatcct cctgcccgcc tgagaacggc aacctgtcca agagccccaa
      241 gcgcgtggca atattttcct gcgtctaaag ggttcgcatc ttttcactgc ttttaaacat
      301 ctaaccggca gcccaatgca actataggta gcaatatctc gaggccgagg aggatcgttc
      361 catgaacaaa cgcaatacaa taatcggcgc cgccgcgctg gtgctagcga ttgcctggta
      421 tgcttttcga ccggagctgt tgttcatcga taggaccgtc aatgaagaat ttcccggagg
      481 cgccgccatg gcatcgatcg agaagggatc gatggagctc acgaagggca cctttaaaag
      541 cctggcgcac gaaaccaagg gattggcttc gatttaccaa ctgtcggacg gcaagcgcac
      601 tctgcgcctg agccaattcg aaacttccaa cggacccgac gtgcacgttt acttgaccgc
      661 tgctgaagtc acgaaaggca gcgaagcgat caaagaagcg ggatttattg atctcggctc
      721 catcaaaggc aacaagggcg atcagaatta tgacattccg gtcaacgctg atttgaataa
      781 atacaaaaac gtcacgattt ggtgtgcgcg ctttagcgtc aatttcggag aggcggcttt
      841 ggacgcgccg gcttctattc ccgtgaaggt tgcggagggg aattttcgcg gcgtcgctca
      901 ccaaaccagg ggaatggcgt cgatctacaa attacctgaa ggaaagaacg tgctacgctt
      961 cagcgcattc gaaacttcca atggtcccga cgtgcacgtt tatcttgtcg cagcgaggga
     1021 tgctacagat gacgaggccg tcaagaaagc tggctttctc gatctcggca gcatcaaagg
     1081 caacattggc gagcaaaatt atgatctgcc aatcgacatt gatctcaaca agtatcgcgc
     1141 cgtcaccgtc tggtgtaagc gtttcggcgt caacttcgcc accgccccgc taattccaac
     1201 caaaagctga aaatattcgc tgcctgttga ggacggtcac ataatcgact ccaactagag
     1261 cgaaagcgta tctttcgata cggcagcgct gctcgcggcc tactcaggac aattggataa
     1321 acgagttccc gagtagcgca aagcgcgtag cgagggatct aatctacact tgatccagac
     1381 aactggcacc agttagcgca tccttcgccg agccatcgac atctaatcag catccgaaac
     1441 ggctatcccg gaggactaca tggcagcaaa cgaccattac gatgtcgtta ttatcggcag
     1501 cggcgctggc ggtggcacac tcgcgcataa gctcgcggcg acgggtaaaa agattttgat
     1561 tctcgagcgc ggcggctacg tgccgcggga aaaagacaac tggaatgcgc gtgccgttaa
     1621 tctcgaagga aaatatcaga ccaaagaaaa atggcatgac gcgagcggca acgagctgca
     1681 cccgcatacc aattattatg tgggcggcaa cacaaaattc ttcggcgcgg cgctgtttcg
     1741 tttgcgcaag gaagatttcg gcgaaatcaa acaccatggc ggcgtgtcgc ccgcttggcc
     1801 gctcacttat gatgagttgg agccgtacta cacggaagca gaaaagctct atcacgtgca
     1861 cggcaatgcc ggcgaagatc cgacggagcc gcgcagaagc gcgccctatc ctcagaaggc
     1921 agtcagccat gagccacgtc tccagcaact ctcggatgat tttgagcggc tcggcttgca
     1981 tccatttcac acgccgctcg gcgtcatgct cgatgaaaaa ctgccccatt tgagtaaatg
     2041 cattcgctgc ggaacctgcg acggttttcc ctgcctgctc tcggccaaat ctgatgctca
     2101 ggtatgtgcc gtcgatccgg ctctgcagaa tcctaatgta gcactgctga ccgatacgta
     2161 tgtcgacaaa ttagccaccg atgcctccgg acgaagaatt tccaaggtgg tagttcgccg
     2221 tcagggtcaa cgcgcggagt tttcggcgga tatcgtcgtc gtttcgtgcg gagcgatcaa
     2281 ctccgctgca ttgctgctcc gatcggcgaa cgacaaacat cctcgcggac tcgccaacag
     2341 ctcggatacg gtcgggcgcc actacatggg acatgtgaat tccgtcttga tggcactttc
     2401 caaatgtccc aatccgacga tctttcaaaa gtcactctcg ctcaacgatt tctatttcgg
     2461 cgccagcggc tggaattatc cgatgggtca catctcattc gtcggtaagc tcgacggcga
     2521 caccttacgc ggcggtgcgc ccgccattgc gccgggctgg actttggatt tgatggccaa
     2581 gcactcgctt gatttctggc tcacatccga ggatctgccc gatcccaaca atcgggtcac
     2641 tgtcgattcc gagggcggag ttgtcttgag ctattcaccc aataaccagg aaggtcacaa
     2701 gcgcttaatc gtcaagctga aagaattgat gcagcaacag agtaaatgca gtgtgcatgg
     2761 tcacaactgc cacgaaggtc tttttgctcg caacctgtac atcggccagc gaattcccct
     2821 tgccggcgtc gctcaccagg tgggcaccgc gcgttttggt agagatccga gaaacagcgt
     2881 gctcgacaca aactgcaaag cccatgactt ggataatctg tacgtcgtcg acgggagctt
     2941 tttcgtatcc agcggcgccg taaacccggc gttgaccatc atggccaatg cgctgcgtgt
     3001 cggtgagcac ttaacggaac ggctcaagta aacttaaaag aaaatgcaaa cgacaaaagc
     3061 acttgcatct cctcgtgttt caacgtggcc tctcgagatg gttaaactat ctcgccttcg
     3121 gacgaaaaca aggtccgtca tgccggcgaa cgccggcatc caggagtggt ggtgaatgaa
     3181 aatacgaaaa gtctggattt cggctttcgc cggaatgacg aaacagaaaa ctcgacatcc
     3241 agtcgactcc aacaacattt ctcggcttcg agctcaggct agcttaattt ttcgtgttct
     3301 ttttcttgtt ttttcctgtt tagtttttcc tttcccggcc gtcgggcagc tcatcaaatc
     3361 cgtcgagacc atcggcatga ccgtttcgaa tatggacggg gcgagtcaat tcttcagcaa
     3421 cgtattgcgt tttgagaaga tcagcgattt cgaagtgtac ggcgccgatt atgaaaaact
     3481 gcagggcctc ttcggtctcc gcatgcgcgt cgtgcgcatg aagctgggag aggaaaccct
     3541 cgagctcaca cagtatctcg cgcccgaggg aagacccatt ccggccgatt ggcgcagtaa
     3601 cgaccgggcg tttcagcata tcgcgatcgt cgttgccgat atggataaag cttaccagca
     3661 cttgcgggcg cacaacgtac gccatgcttc tacggctccg caactgattc cggcagccaa
     3721 cccggccgcc gccggcattc gcgcttttta cttcaaagat ccggatggcc attatttaga
     3781 gctgatccat tttccccaag gcaagggtga cccgcgctgg cagcaaaaga gtgataagtt
     3841 gtttctcggc atcgatcaca cggcggtcgt cgtgtcgagc acggaaaaca gcctgagata
     3901 ctaccgggac ttgctcggca tgcggctcgc gggagagagc atgaattccg gaaccgagca
     3961 ggagcatctg aacaatgtcg ccggtgctcg cttgaggatc agtggtttgc gcgcaatcag
     4021 tggtcccgga atcgagttcc tcgagtatct ttcacctcga aatggccggc ctgccccgaa
     4081 ggacacgcgc gccaatgata tttggcattg gcagacgatg ctcaggactg gcaacgccga
     4141 aaagacattc agaaaactta atcgcggtag cggacgcgcc gtttcttccg gcatcgccga
     4201 gatttccgag gctgatttcg gcttcaccaa aggatttctt gcgcgcgatc ccgacggcca
     4261 cggtttgcag attgttgaga agtgattcta ttgctgcgta ccaagtcaga atcg
//
LOCUS       DASULZ010000488         3016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1176440, whole genome shotgun sequence.
ACCESSION   DASULZ010000488 DASULZ010000000
VERSION     DASULZ010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176440"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VFY96_09975"
     CDS             <1..231
                     /locus_tag="VFY96_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEX5606832.1"
                     /translation="AGEGASTIGDKTVEWTKHDSFALPNWAWHEHVNRSKSEEAILFS
                     VNDIPMVEAFGLYREEPENSLHAAPAPAVPQS"
     gene            373..1806
                     /locus_tag="VFY96_09980"
     CDS             373..1806
                     /locus_tag="VFY96_09980"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX5606833.1"
                     /translation="MPDLRTWLADVEKLGQLMTIENIHWDLELSTLTEIINERSKTRP
                     AIIFDRIKDYPQGYRVAANLVSSVERLALTLGMKAGLSDMDFVQTWRQQVKKIAPLRA
                     ETVSSGPLFENIKKGKDINVFDFPVPRWHELDGGRYIGTDDLVITRDPDEGWVNVGTY
                     RIMVHGPDRLGLHMSPGKHGRVHRDKYHAQGKPLPIAVSFGHHPINFVVASTDVPNRV
                     NEYEYAGGILGRPLQVINGPMTGLPVPADAEIAIEGEVAVDDVMPEGPFGEWTGYYAS
                     NQPAVPVIQVKALYFRNDPILCGFPLLKPSSGDNLHFSLMRSALIWNALDEAGVPDVK
                     GVWCHPAGGRFLTVVAITQRYPGHARQAGMIASQCRSGAYLGRYVVVVDDDVDITNSE
                     EFLWALSSRSDPAESIEILRRAWSGPLDPRIARDQVGHSSRAVIDATRPYEWREKFPK
                     VSGASRELKDKVAGNWREFLDQKIGRP"
     gene            1885..2472
                     /locus_tag="VFY96_09985"
     CDS             1885..2472
                     /locus_tag="VFY96_09985"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX5606834.1"
                     /translation="MSNSEQKVYLLRHGETEWSLNGRHTGITDIPLTDNGRRIARLLQ
                     PILARETFAMILTSPLQRARETCELAGLGSLATIDRDLMEWNYGDYEGLTTEQIRQTN
                     PNWSVFRDGCPGGESPLEIGVRADRVLARIRATDGNVALFAHGHILRVLAARWINLSA
                     TYGEHFLLDTATLNVLGYYRESPAFKIWNAPLQNS"
     gene            2664..>3016
                     /locus_tag="VFY96_09990"
     CDS             2664..>3016
                     /locus_tag="VFY96_09990"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008387596.1"
                     /note="catalyzes the interconversion of alpha-D-glucose
                     1-phosphate to alpha-D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase, alpha-D-glucose
                     phosphate-specific"
                     /protein_id="HEX5606835.1"
                     /translation="MAIHPLAGKPAPEEILINVGELERAYYERVPDISNPHERVSFGT
                     SGHRGSPFDGTLNEAHILAITQAICDYRAMKKISGPLFMGKDTHAVSGPAQRSALEVL
                     AANGVETVIQRDDGFT"
BASE COUNT          681 a          846 c          861 g          628 t
ORIGIN      
        1 gctggtgagg gtgccagtac catcggcgac aagacagtgg aatggacaaa gcacgatagc
       61 ttcgcgctac ccaattgggc ctggcatgag catgtcaacc gatcaaagag tgaagaagcg
      121 attttatttt cggtcaatga cattcccatg gttgaagctt tcggcctcta tcgagaggag
      181 ccggagaatt cgttgcatgc agcgccagcg ccggcggtgc cgcaatcata agataaccgg
      241 cgttcaagag gttccagacg ttcgaacagt tcaaaacgtc gcaccggatc cgcctgaacg
      301 atttaagcgg agcgaagcga ttgaacggct tcaaccgctc gaaccgatct gaagtcatcg
      361 ttgaaaggac ttatgccaga cctacgcaca tggcttgctg acgttgaaaa gctcggtcag
      421 ttgatgacga tcgagaatat ccattgggat ctcgagctct cgaccttgac ggaaattatc
      481 aatgaacgct cgaagacgcg gccagcaatt attttcgacc gtataaaaga ttatccgcag
      541 ggctacagag tcgcggccaa tctggtttct tcggtggaac gactcgccct gacgctcggg
      601 atgaaagccg gtttgagcga tatggatttc gtccagacct ggcggcagca ggtaaagaaa
      661 attgctccgc tccgcgcgga aactgtttca agcggaccgt tgttcgagaa cataaaaaag
      721 ggaaaagaca tcaatgtgtt cgattttccc gtgccgcgtt ggcacgagct tgacggcggc
      781 cgttatatcg gcaccgacga tctggtgatt acccgggatc ccgacgaagg ctgggtgaac
      841 gtcggcacct atcggatcat ggttcatggc cccgatcgcc tggggttgca tatgtctccc
      901 ggcaaacacg ggcgcgtgca tcgggataaa tatcacgcgc agggaaagcc gttgccgatc
      961 gcggtttcgt ttggccatca cccgatcaac ttcgtggtgg catcaaccga cgtgcccaat
     1021 cgggtgaatg aatacgagta tgccggcggc attctcggca gaccgctgca agtcatcaac
     1081 ggtcccatga cgggtttgcc ggtccccgcc gatgcggaga tcgccatcga gggcgaagtt
     1141 gctgtagacg atgtgatgcc cgagggcccg ttcggtgaat ggacgggcta ttatgcaagt
     1201 aaccaacccg cggtgccggt gattcaagtc aaagcgctct atttccgaaa cgacccgatt
     1261 ctctgcggct tcccgttgtt gaaaccctcg agcggcgaca atctgcattt ttcattgatg
     1321 cgctcagccc tcatctggaa tgcgctcgat gaagcgggtg tgcccgacgt taaaggcgtc
     1381 tggtgccacc cggcgggcgg gagatttttg acggtggttg caatcacgca gcgctacccg
     1441 ggacatgcgc gccaggcggg catgatcgcg agtcagtgcc gctcgggcgc ctatctgggg
     1501 cgctacgtcg tcgtcgtcga tgatgacgtg gatatcacca attcggaaga atttctctgg
     1561 gcgctgagca gccgctccga tcccgccgaa tcaatcgaaa tccttcgccg cgcctggagc
     1621 ggcccactcg acccgcgcat cgcgcgcgat caggtcggcc acagctcgcg cgccgtgatc
     1681 gacgctacgc gcccttacga gtggcgcgaa aaatttccca aagtcagcgg cgcgagccgg
     1741 gagttgaaag acaaagtcgc cggaaattgg cgcgagttcc tggatcagaa aatcggtagg
     1801 ccgtagcgtg cataatacca ggcgggatta gtgagttgag ttacgctgcg ctaacccaac
     1861 ctaccggatg gaattgctag cgctgtgtct aactcggaac aaaaagttta tctcctccgt
     1921 cacggcgaaa ccgagtggag cctgaacggg cgacacaccg gaatcaccga tattccgctc
     1981 accgacaatg gccgaagaat cgcccgacta ttgcagccga ttcttgccag agaaacattt
     2041 gcgatgattc tcaccagccc gctccaacgg gcgcgcgaaa cctgcgagct cgcggggctt
     2101 ggcagcctgg caactatcga tcgtgatctc atggagtgga actacggcga ctacgagggc
     2161 ctcacgaccg agcaaattcg ccagacgaac ccaaattggt cggtttttcg cgatgggtgt
     2221 ccgggcggcg aatcgccgct agaaatcggc gttcgcgccg accgggttct cgcaagaatc
     2281 cgcgctacgg atggaaacgt cgcgctcttc gcacatggtc atatcctgcg cgtgctagct
     2341 gctcgctgga tcaacttatc ggccacctac ggtgaacact ttctgctcga tacggcaact
     2401 ctgaatgtgc ttggatatta ccgcgagtcg ccggcattca aaatctggaa cgcgccactc
     2461 caaaactcat aattgctagc cagcgaactc cagccttgcg tcgccaatag ccgcacattc
     2521 cttttctgtt taattcgcag cgctcccctg cgtttcgttt ttttgtcgcg cacggacagc
     2581 agctatcatc agcgttgtcg ccattgtata aatcacctag caaatccgag tggaggcatc
     2641 tgcggttcag aagggaattt cccatggcga ttcatccatt ggcgggaaaa ccggcgcccg
     2701 aggagatctt gatcaatgtc ggtgagctcg aacgagctta ttacgagcga gttccagata
     2761 tcagtaatcc gcacgagcga gtgagcttcg gcaccagcgg tcatcggggc agccccttcg
     2821 acggcacgct caacgaggcg cacattctcg ccatcacgca agcgatctgt gattaccgtg
     2881 cgatgaagaa aatatccggg ccgttgttca tgggtaaaga cacccatgcc gtctcgggac
     2941 ctgcgcagcg aagtgccctc gaggtgctcg ccgccaacgg cgtggaaacc gtcattcagc
     3001 gcgacgacgg ctttac
//
LOCUS       DASULZ010000489         2510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1176643, whole genome shotgun sequence.
ACCESSION   DASULZ010000489 DASULZ010000000
VERSION     DASULZ010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176643"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="VFY96_09995"
     CDS             complement(<1..1044)
                     /locus_tag="VFY96_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEX5606836.1"
                     /translation="MTAEFNGEEENRLRPFFTNLDRSVFGLKLPQEVAGALFSRYSRS
                     TKSLRRTFLDEFLGDPELGLKNLLAGERPASDDSSALKKARAFYERVLVGYGDDSVAQ
                     LGGAHLACENISNVAAKLLEDARIGIAPLEKSTRYVRFDQKDAAGNYLFYREPKIMAS
                     RHRDAYLEGMNLLFETYSKQMEPMLECVAQSLPIEQLEVRDPTTGKSLAYAEAHKDER
                     LRRWAETAYRATVRAHACDVLRSYLPAATLTNVGMFGVGQAFEYLISKFYSHELSEAM
                     ELGKAMHGELNQLIPSFVKRAQLNEYLAGTSAAGKALASKTAHIRPLDKKEPVALIDC
                     DNAAEEKIIAAMLY"
     gene            complement(1144..2277)
                     /locus_tag="VFY96_10000"
     CDS             complement(1144..2277)
                     /locus_tag="VFY96_10000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5606837.1"
                     /translation="MKIFDAVIVGAGPAGSATAIGLARRGYEVALIDKQNFPREKLCG
                     DFINPINWDVFADLGVADRVLAAPHGEVTGFRITHYSGRQAEARFRSRDLKPPMGLGL
                     RRATLDRLLVERAAELGAVTQLGCRIENLSRIAHSWHLKIKEESWQAKVLIGADGRNS
                     WVAQQLGLNRSAAIQGRSVGFQSRLRCTGVTCGQIQIHLFPGGYAGIVELGDGTITVG
                     MAIDKRRLARERGTDFVFKQLAQNIYLKQIIERSSGAAQLSSVYPVYFPKRRSVTDHA
                     LLVGDAARVSEPVTGEGIYFAMRSGLLAAETVDLAVRQKNLGANGLAGYERACRRAFR
                     SRLALNSLLRFAVYRPALVDPLIRLSAKKGRVLDSLVDAVCIP"
     gene            complement(2278..>2510)
                     /locus_tag="VFY96_10005"
     CDS             complement(2278..>2510)
                     /locus_tag="VFY96_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606838.1"
                     /translation="IIVNDLVRHRLAYQGIRLVTKTFTRNEMTRTDAPRSVERACTIS
                     EWHELFRRANVGKFSVQWAVPFRVLGVIAISR"
BASE COUNT          526 a          722 c          728 g          534 t
ORIGIN      
        1 gtaaagcatc gcggcgataa tcttttcttc ggcggcgttg tcacagtcga tgagcgcgac
       61 cggctctttt ttatcgagcg gccggatatg ggctgtttta gaggccagcg ccttgccagc
      121 ggctgaggtc ccggcaagat attcgttcaa ctgcgctctc tttacgaaag acggaataag
      181 ctgattcagc tcgccgtgca tcgctttgcc gagttccatt gcttcgctca gttcgtgcga
      241 ataaaatttg ctgatcagat actcgaaagc ctgaccgacg ccgaacatgc ctacattcgt
      301 caaggtcgct gccggcaagt aactccgtaa aacatcgcac gcatgggcgc gcacggtggc
      361 gcgataggcg gtttccgccc agcggcgcag ccgctcgtct ttgtgcgcct cggcgtaggc
      421 aagcgacttg ccggtagttg gatcgcggac ttccaactgc tcgatcggca acgattgcgc
      481 cacacattcg agcatgggct ccatctgctt cgagtaagtc tcgaagagaa ggttcatgcc
      541 ttccaggtat gcgtcgcggt ggcgcgaggc catgattttg ggctcacggt agaagagata
      601 atttcccgcg gcgtctttct ggtcgaagcg cacgtagcgc gtcgattttt ccagcggcgc
      661 gatgccgatg cgcgcgtctt cgaggagttt ggccgccaca ttggaaatat tttcgcaggc
      721 aagatgcgcg ccgcccagtt gggcgacgga atcgtcgccg taccctacca gcacgcgctc
      781 gtaaaacgcc cgagcttttt ttagcgccga gctgtcgtcg ctggcgggcc tttctccagc
      841 gagcaagttt ttcaagccaa gctctggatc gccgagaaat tcgtccagga acgtgcggcg
      901 taagcttttc gtcgagcggc tatagcggga gaacaaagcg ccggccactt cttgcggcag
      961 cttgaggccg aaaaccgagc gatcgaggtt ggtgaaaaaa ggacggagac gattctcctc
     1021 ttcgccatta aactccgcgg tcatttttta atagtcctcg atgtaaactg ggcgccattt
     1081 tattcagttc tggcgtaatg acaacctgtt aattcttaaa acgagtaggt aagctccgct
     1141 cgcctatggg atgcaaaccg cgtcgaccag cgaatcgagc acgcgccctt ttttcgcgga
     1201 cagccgaatg agcggatcga ccaaagctgg acgatagacg gcaaaacgca ggagggagtt
     1261 caacgccaag cgcgagcgga aagcgcggcg gcaagcgcgc tcgtaacccg ccaaaccatt
     1321 cgcgccaaga tttttctgcc tgacagcaag atcaaccgtc tccgccgcga gcaagccgct
     1381 gcgcattgcg aagtaaatgc cttcgccggt gaccggctca gagacgcgcg ccgcatcgcc
     1441 aaccagcaac gcgtggtcag taacggagcg gcgctttgga aagtagaccg gatagacaga
     1501 gctaagctga gcagcgccac tgctgcgctc gatgatctgt ttcaggtaga tattttgcgc
     1561 caattgttta aagacgaagt cggtgccgcg ttcgcgggcg agccggcgtt tatcgatcgc
     1621 catcccgacg gtgatcgtgc cgtcgcccag ttccacgatc ccggcatagc cgccgggaaa
     1681 gagatggatc tggatttgcc cgcaagtcac gcccgtgcag cgcaaacggc tttgaaaacc
     1741 gaccgaccgt ccctgaatgg cggcgctgcg atttaaaccg agctgttgcg caacccatga
     1801 gttgcgccca tcggcgccaa tcaaaacttt tgcttgccag ctttcctctt tgattttgag
     1861 gtgccagctg tgcgcgattc gggaaaggtt ttcgatccgg cagcccaact gtgtgaccgc
     1921 gcccaactct gccgctcttt ccaccagcag ccgatcgagc gtcgcgcgcc gcaatccgag
     1981 acccatcggc ggtttgagat cgcgtgacct gaagcgggcc tcagcttggc ggcccgagta
     2041 atgggtgatg cgaaatcccg tcacttcgcc gtgcggcgca gcgagcactc gatctgccac
     2101 accaagatcg gcaaagacat cccagttgat cgggttgatg aaatcgccgc agagcttctc
     2161 ccgaggaaag ttttgcttgt cgatcaatgc aacctcgtag cctctgcgag ctaggccgat
     2221 tgccgttgcg cttccggccg gaccggcacc gacaatgact gcgtcgaaga ttttcattta
     2281 gcggctgatc gcgatcactc cgaggactcg gaatggaacg gcccattgga cggaaaattt
     2341 ccctacattg gcgcggcgaa acagctcgtg ccactctgag atcgtgcagg cacgctccac
     2401 cgaacggggg gcgtccgtcc gtgtcatttc atttcgtgta aaggttttgg taacgaggcg
     2461 gatgccctga taggcgaggc gatggcgcac caaatcgttc acgatgatcg
//
LOCUS       DASULZ010000490         6696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1073579, whole genome shotgun sequence.
ACCESSION   DASULZ010000490 DASULZ010000000
VERSION     DASULZ010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6696
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073579"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VFY96_10010"
     CDS             <1..188
                     /locus_tag="VFY96_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606839.1"
                     /translation="LVFSQLVPEAIPAPGDFSVGKFELTPFDLFPSEAAAEELFATIH
                     EYVGWVNYYSRLRFFGT"
     gene            328..1374
                     /locus_tag="VFY96_10015"
     CDS             328..1374
                     /locus_tag="VFY96_10015"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5606840.1"
                     /translation="MKFYKSGHDLVRAVDGVSIEVGAQEFFVLLGLSGSGKTTLLRCV
                     AGLEKPEGGEILIHQKLVSSAERNYFIGPEERGIGMVFQSYAVWPHMTVYQNVAFPLI
                     NGPHRYSSTEINSRVTNALELVQLAALADRPAPFLSGGQQQRVALARALAVEPKVLLM
                     DEPLSNLDARLREEVRDEIKTLAKKFGIAVLYVTHDQVEAMALADRVAVMSKGKVLQC
                     AAPRDLYDAPSSREVCEFLGATNLLDGMICNGGRIETELGQITCVVTDPAFKNVSVAI
                     RPEEIQLLPASTGLENEFPAEVLSSTFLGELTVCELLVNGKKLRFKTTQSAAPCARFH
                     IRLPADKLKVFARN"
     gene            1406..2683
                     /locus_tag="VFY96_10020"
     CDS             1406..2683
                     /locus_tag="VFY96_10020"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5606841.1"
                     /translation="MLPREDNDLLTQVGPEKPLGKLMRHYWLPALLSEEIPEPDCPPV
                     RVRLLGENLVAFRDSQGRVGLLGEHCAHRGTSLFFGRNEECGLRCIYHGWKYAVDGTV
                     LETPAEPSGSTLKDKVRQTAYPCKEAAGIVWSYLGPPDKLPLLPNYEWMHLPRKNLYV
                     TKSVQDCSWLQGLEGECDSSHLSFLHKNFTGERPRGGGDGALYAADSAPQLQGIEMDY
                     GVRMLSCRKIAPDTVYLRVSNIVLPCHGFIPTGGLKGNPEGYTIHSHVPIDDSHSMRY
                     NMHFRRNRAIEDDERQHDEEIGSDFHKRRNLANNYLQDREEQKHENFTGMGPIFLVHD
                     SCATESMGPIYDRAQEHLGVSDMTVIAVRRFLLRAVRAVAAGQEPPHVIRTEAQNDLR
                     HLACIASKIPAHWDPNLYVTEQLKKENYWETNS"
     gene            2854..4497
                     /locus_tag="VFY96_10025"
     CDS             2854..4497
                     /locus_tag="VFY96_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018303103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HEX5606842.1"
                     /translation="MKVVDVIAEILKREGVEFLSCYPTNTLIEAAASAGIRPVVCRQE
                     RVGVGIADGFTRVTNGRRIGVFTMQAGPGAENAFAGITTAYSDSVPVLLLPVGHARNT
                     SQVFHFFRSVQAYAPVTKWIEEITLADQVPEIMRRAFTHLKMGRFGPVLVEIPNDVAV
                     EEFPAAKIDYTPVKRALSSANAADVEAATKILVEAKRPVIVAGQGVLYAEASDELVEL
                     AELLQAPVTTPLEGKSAFPEHHPLALGTGAGVMPRTVHAFLRKADLIFAVGASLTKHS
                     IVAAPIPAGTTIIHATNDERDINKHYATDYPIVGDAKPTLRQFIEAVKDALGKNQRKP
                     NGIASEITEIREAWISEWMPRLTSNEKPINPYRVMWEFINLFDPKDVIVTHDAGSPRN
                     QLVPFYRAATPRSYLGWGKSHALGTGLGLIMGAKLAAPNKFCVNFMGDAAFGMTGLDF
                     ETAVRNNIPTLTIVLNNNFMAAETHSMHASHERYGTMNILGNYSEIAKSLGGWSERIE
                     EPGQIVSALQHARKVTEDGKAALLEFVTSKEINYSRMRD"
     gene            4973..5908
                     /locus_tag="VFY96_10030"
     CDS             4973..5908
                     /locus_tag="VFY96_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX5606843.1"
                     /translation="MDSGRIFLWIGFNVFVLALLAMDLGVFHRKAHSVSIKEATAWSI
                     VWVSLAMIFNLAIYFTWGEEKALEFFTGYVIEKSLSVDNLFVFVMIFQYFSTPAEYQH
                     RVLFWGILGALILRAIFIAAGSALLNQFHWMIYVFGAFLVFTGIKMFLQGDEKLEPRR
                     NPVVRLFERWVPMTREYHGQSFMIRINGQTRATLLLLVLIVIETTDVIFAVDSIPAIF
                     AVTQDSFIVYTSNVFAILGLRALYFMLAGVIQMFVYLKFGLSLVLCFVGAKMLLSDIY
                     KIPIGASLAVIGTVLLLSIIASWWTQRKSPATQST"
     gene            complement(5905..>6696)
                     /locus_tag="VFY96_10035"
     CDS             complement(5905..>6696)
                     /locus_tag="VFY96_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606844.1"
                     /translation="MFEWNKWSLFAPPLNTTHRLLNGSREPVRFLAVTNAPLAFDIYR
                     DESFVFDCPYSFKDRYYGEENYFEVGKKRYVSGLINIWETNFIPDIKTAMLESLEVKG
                     AGLRLAQFEMSGNALVGHLGEWPVGKYHKAHYHGPGAIIVGTQSVGYVLLWHKQWGIH
                     PYADRHEDRVIEIEWGEASIYCPPGDWFHQHFNTGKEPARHLAVRYGSRIHPLGFKVS
                     HQKTDDDVFRSVDEGGWMIPYENEDPEIARRYQAALRKNGVPQPF"
BASE COUNT         1499 a         1835 c         1815 g         1547 t
ORIGIN      
        1 tgctggtgtt ttcacaactt gtgccggaag cgattcccgc tcctggcgat ttttcggttg
       61 gaaaattcga gctcaccccc ttcgatctct ttcccagcga agccgcggcc gaagaactat
      121 tcgcgaccat tcacgaatat gtcggctggg tgaattatta tagtcgtttg cgtttcttcg
      181 gcacttgagc cttttttccc gtcaatgcgt acagcaaagt ccatttgcaa aacggttagg
      241 gagtgataga aacagccgtt gacctggcgc cattcgcggt gaaaaacgcc ccttcgtgtt
      301 ccagcaatga tatccatcag gaaccttttg aaattctata aatccggcca tgatttggtg
      361 cgcgccgttg acggagtttc catcgaggtc ggcgcgcaag agttctttgt actgctgggg
      421 ctaagcggct caggaaagac aaccttactg cgctgtgtcg cgggcctgga gaagccggaa
      481 ggcggcgaaa ttctgatcca tcaaaagctg gtcagttctg ctgagcgaaa ttattttatc
      541 ggtccggagg agcgcggtat cggcatggtt tttcaatcct atgccgtatg gccgcacatg
      601 accgtatatc agaacgtcgc gtttcccttg atcaacggcc cgcatcgcta ctcttccacc
      661 gagataaaca gccgggtcac aaacgcgctc gagctcgtcc aactcgccgc tctcgcggac
      721 cgtccagcgc cgtttttaag cggcggacaa cagcaacgtg ttgccttggc tcgggcgctc
      781 gcggtggaac cgaaagtgct actgatggat gagccgttga gcaatctcga tgcgcggcta
      841 cgcgaagaag ttcgcgatga aataaaaaca ttggcgaaaa agttcggcat agccgtcctt
      901 tacgtaaccc acgatcaggt cgaagccatg gctctggcgg atcgtgtggc agtcatgagc
      961 aaaggcaaag tgctgcagtg cgcagcgcca cgcgacctct acgacgcgcc ttcaagtcgg
     1021 gaggtttgtg agttcttggg cgcgaccaac ttgctcgacg gcatgatttg caacggtggg
     1081 cgaattgaaa ccgaactggg acagatcact tgtgtggtta cggatcccgc gttcaaaaat
     1141 gtaagcgtcg cgatccgtcc ggaagaaatc caacttctac cggcatcaac cggcttggaa
     1201 aatgaatttc ccgccgaagt gctatcctcg acttttctgg gcgaattaac cgtatgcgag
     1261 ttgctggtca acggaaaaaa gttgcgcttc aagactacgc agagcgccgc tccgtgcgca
     1321 agatttcaca tccgcctacc ggcagataag cttaaggtgt tcgcccgaaa ctaaatggga
     1381 tttttcgcga agagaggaaa gccccatgct cccgcgagaa gacaacgatt tactcacgca
     1441 agtcggtcct gaaaagccgc tgggcaaact gatgcggcat tactggcttc ctgctttgct
     1501 gtctgaagaa atacctgagc ccgattgtcc tcccgtccgc gttcgtctgc tcggcgaaaa
     1561 tctggttgcg tttcgcgaca gccaggggcg cgtcggattg ttgggtgaac actgcgcgca
     1621 ccgcggcacg tcgctctttt ttggacgaaa cgaggaatgc ggactgcgct gtatttacca
     1681 cggctggaaa tatgccgttg acggcacggt cttggaaaca ccggcggaac cgtccggcag
     1741 cacattgaaa gataaagtgc gacagaccgc atacccctgc aaagaggcgg cgggaatcgt
     1801 ttggagctat ctcggccctc cggataagct gccgcttttg ccgaattacg agtggatgca
     1861 tctgccgcgc aaaaatctat acgtaaccaa atcggttcag gattgtagct ggttgcaggg
     1921 gcttgaggga gaatgcgact catcgcatct ttctttcctg cacaagaatt ttactgggga
     1981 gcggccgcgc ggcggcggcg acggcgcact gtacgcggcc gactctgcgc cacagttgca
     2041 aggcatcgag atggattacg gcgtgcggat gctttcctgc cgtaagatcg cccctgacac
     2101 cgtgtatctg cgggtgtcga acatcgtgtt accctgccat ggatttattc ccaccggcgg
     2161 attgaaaggc aatccggaag gctacacgat tcactctcac gtgccgatcg atgacagcca
     2221 cagcatgcgc tataacatgc actttcgccg caatcgtgcc attgaagatg acgaaagaca
     2281 gcacgatgag gagatcgggt cagattttca caagcggcgc aatctggcga ataactattt
     2341 gcaagaccgc gaggagcaga aacacgagaa ctttaccggc atgggaccga tttttctcgt
     2401 ccatgattcc tgcgcgacgg aatccatggg tccgatctac gaccgcgcac aagagcatct
     2461 cggtgtcagc gacatgaccg ttattgcggt gagaagattc ctgttgcgcg cggttcgtgc
     2521 tgtcgcagcg gggcaggaac cgccccatgt gatccgcacc gaggcgcaga acgatttgcg
     2581 gcacctcgcc tgcattgcca gcaaaattcc cgcgcattgg gatccgaact tatacgtcac
     2641 ggaacaactg aaaaaagaaa actattggga aacaaactcg tagagcaggt ggtaacgcct
     2701 acattccggg cgagtaccat ggggtatttg taaattacca tgcctgtcat gttgagcgca
     2761 gcgaagcatt tcgagttctt aacctcgaag acgagatcct tcggctgtcg tcttaggatg
     2821 acatcataag acgccagcta gtcgggaggt attatgaaag ttgtcgatgt gattgccgag
     2881 attctcaaac gtgaaggcgt agagtttctc tcctgctacc cgacaaacac cctcatcgaa
     2941 gccgcagctt cggccggcat tagaccggtt gtttgccgac aagagcgggt cggcgtgggc
     3001 atcgcagatg ggttcacgcg cgttaccaac ggccggcgca tcggcgtttt tacaatgcag
     3061 gccgggcctg gggccgaaaa cgcatttgcc ggcatcacca ccgcctattc cgattccgtc
     3121 ccggtgctgc ttctgccggt cggccatgcg cgcaatacgt cccaagtctt tcattttttt
     3181 cgttccgttc aagcgtatgc tcccgttacc aagtggatcg aggagattac gcttgccgat
     3241 caagtgccgg agatcatgcg gcgcgctttt actcatctaa aaatgggacg gttcggaccg
     3301 gtcctcgtag aaattccgaa cgatgtcgca gtggaggagt tccctgcggc aaagatcgat
     3361 tacacgccgg tcaaacgagc gttgagtagc gccaacgctg ccgacgtcga ggcggcgacc
     3421 aagattttgg tcgaagccaa acggccggtg atcgttgccg gtcagggcgt gctctatgcg
     3481 gaagcatccg acgaactggt cgaactggcc gagctattgc aagcgccagt gacaactccc
     3541 ctcgaaggga aaagcgcttt ccccgagcat catccattag cgcttggcac cggcgccgga
     3601 gtcatgccgc gcaccgtgca tgcattcttg cggaaggccg acctcatttt tgcggttggg
     3661 gcgagcctca cgaagcacag catcgtggcc gcgccgattc cagccggaac gaccatcatt
     3721 catgcgacca atgacgaacg cgatattaac aagcattacg ccacggatta tccgatcgtc
     3781 ggcgatgcca aaccgacctt gcggcagttc atcgaagccg tcaaggatgc gcttggaaag
     3841 aaccagcgca agcccaacgg cattgcgagc gaaatcacag aaatccgaga ggcatggatc
     3901 tccgagtgga tgccgagact cacttccaat gagaaaccga tcaatcccta cagagtgatg
     3961 tgggaattca taaatctttt cgaccccaaa gatgtgattg tcacccatga cgcaggcagt
     4021 ccacgcaacc aactcgtgcc gttttaccgc gcggccacgc cgagaagtta tctcggctgg
     4081 ggcaaatcgc atgcgcttgg caccggtttg ggcctgatca tgggcgcgaa gcttgctgca
     4141 ccgaacaagt tctgcgtcaa ctttatgggc gatgctgcct tcggcatgac cggattggat
     4201 ttcgaaaccg cggtgcgcaa caacattccg accttgacga tcgtcttaaa caacaacttc
     4261 atggccgccg aaactcacag tatgcacgcc tcgcatgagc gctatggcac aatgaacatt
     4321 cttggcaatt actccgagat cgccaaatca ttaggtggct ggtctgaacg gattgaggaa
     4381 ccgggtcaga ttgtctccgc tctgcaacat gcgcgtaaag ttaccgagga cggcaaagcg
     4441 gctttgctcg aattcgtgac cagcaaggaa atcaactatt cgcgcatgag agactagccg
     4501 cagttcctag agccctcggc ggttcgacgt acgcggtgct aggcatgccg agcgccccca
     4561 caaagccagc cggcttgaat taagcattcg aggttgctag ccgttcttgg atgaaacggc
     4621 cacgccttgc gtcatggcta gtaatcctag tcgggatcgt gctcggcggg agccatgtgt
     4681 tcggcttgca cgcgttcagc ccaactgcgt tcgaggaaga gagcttttcc gacgagtccg
     4741 agcaaaagca tagcacgata caaagagccg ttagggccaa acgcattcgc tcgaacaaaa
     4801 ctcaggcgag tcagccgcca tcgttttcgc cgctcgcgca ccggccacca cttccctacc
     4861 tccgtgtgcg ctcgtttctc aatctgcgct cggttccgcc aattttaaga atataggaac
     4921 tctctctttc tgccgcattc gccagcttca gtggtgaaag ggagaatgga acatggattc
     4981 cggtcggata tttctttgga ttggctttaa cgtatttgtg ctcgcgctcc tggcgatgga
     5041 tctcggtgta tttcaccgca aagcccattc cgtctccatc aaggaagcga cagcttggag
     5101 cattgtctgg gtttcgctcg cgatgatctt caatcttgcg atttacttca cctggggaga
     5161 ggagaaggcg ctcgaattct tcaccggcta cgtgattgaa aaatcgctca gcgtcgacaa
     5221 tttatttgtc ttcgtcatga tctttcaata tttctccacg ccggcagagt atcagcaccg
     5281 cgtgcttttc tggggaattc tcggcgcgct catactgcgc gccattttta ttgcagccgg
     5341 ctcggcattg ctcaatcagt ttcattggat gatttatgtt ttcggcgcgt ttttggtgtt
     5401 taccggcatc aaaatgtttc tccaaggcga cgaaaaactc gagccgaggc gaaacccggt
     5461 ggtgcggctg tttgagcgct gggttccgat gaccagagaa tatcacgggc agagcttcat
     5521 gattcggatc aatggtcaga cgcgcgccac gttgctactg ctggtcttga tcgtcattga
     5581 aactaccgat gttatcttcg ccgtggattc cattcccgca atcttcgccg tgacgcaaga
     5641 ttcgtttatc gtttatacct cgaatgtttt cgctattctc ggtctgcgcg ccctctactt
     5701 tatgctcgcc ggcgtcatac agatgttcgt ttacctgaag ttcggacttt ccctagtcct
     5761 ctgctttgtc ggcgccaaaa tgctcttatc cgatatttat aaaattccca taggcgcctc
     5821 gttagccgtg atcggcacgg ttttactgct ttccatcatc gcatcgtggt ggacgcagag
     5881 gaaatcgcca gcgacgcaaa gtacctagaa aggttgaggg accccgttct tccgcagcgc
     5941 agcctgatag cgtcgcgcaa tttccggatc ctcgttctcg taaggaatca tccagccacc
     6001 ttcatcgacg ctgcgaaata cgtcatcgtc ggtcttctgg tgtgagactt tgaaacccag
     6061 cggatggatt cggctgccgt agcggacggc gagatggcgc gccggctctt tcccggtgtt
     6121 gaaatgctga tgaaaccagt cgccgggcgg gcagtaaatg cttgcctcgc cccattcgat
     6181 ctcaatcact cggtcctcgt gtctgtcggc atacggatgg atgccccatt gcttgtgcca
     6241 tagcagcaca taaccgacgg attgcgtgcc gacgatgatc gcgccgggcc cgtgatagtg
     6301 cgccttatga tattttccca ccggccactc gcccagatgg ccgaccaagg cgttgcccga
     6361 catttcgaac tgcgccagac gcaaaccggc gcctttcact tccaaactct caagcatggc
     6421 ggttttgata tctggtatga agttggtctc ccagatgttg atcaagccgc tcacatagcg
     6481 cttcttgccg acttcgaagt aattttcctc accgtaatag cggtctttaa agctataggg
     6541 acaatcgaaa acaaaactct cgtctcgata gatgtcgaag gcgagcggcg cattggtgac
     6601 cgcgagaaag cgcaccggtt cgcgcgagcc attgagtaaa cgatgcgtgg tattcagcgg
     6661 cggcgcaaag agactccact tgttccattc gaacat
//
LOCUS       DASULZ010000491         3593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_770001, whole genome shotgun sequence.
ACCESSION   DASULZ010000491 DASULZ010000000
VERSION     DASULZ010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770001"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(412..1932)
                     /locus_tag="VFY96_10040"
     CDS             complement(412..1932)
                     /locus_tag="VFY96_10040"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEX5606845.1"
                     /translation="MKSSTTVRPGCVDLPAFPLQLLLRRHPEWRAYPAAVVDEDKPQG
                     LLLWVNEKARRQGVLPGLRYAAAFSLASTLRAGQVLPAEIDKAVGELVKALMRFTPEV
                     EPAREEPGVFWLNGAGLQHLYYSPQQWAHAIYQNLRRRGFAVNLAVGFTRFGTYATVK
                     AKGGVTVFHDPETERNAARKAPLQRLNIEPEFRDALFKLGIKTVSDLLLLPSGGLRER
                     FGKEAHRLYRMAVGDLWMPLEPESPEEPLTKKYILDDPEDDATRLLFVIKQLLNPLLA
                     ALAKRYQALVTLWISLVIDHRNRIRESLRPASPTLEAVQITDLIRLRLESLQLDAGVV
                     EIELTVESCAATREQLFLFERSVLGQLEGSRRNLEAANRALARLRAEFGDESVVCAKL
                     NDGHLPEARFTWEPLHRVQSPKPKASTVKTLIRRICTKPGTIDGAPLRSHEDGWLLGA
                     KSGAVDKLAGPYIFCGGWWNREIQREYYYAETRNGDLLWLYYDRIRRKWFLQGAVE"
     gene            complement(1895..2494)
                     /locus_tag="VFY96_10045"
     CDS             complement(1895..2494)
                     /locus_tag="VFY96_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase A"
                     /protein_id="HEX5606846.1"
                     /translation="MVLSPAPDLQFSSPSETPWRLDQVAGRFIEISSTGASAALTLTF
                     SLIDEAQQRGEPVGWVASTKDFFYPPDAARNGIDLAALVVIRLTEPTCIARAGEKLLR
                     SGGFGLIVLDLGAADIAMPLQTRLTGLAHRHHTALVCLTKKNRAASSLGSLVSLRAHA
                     QKKRMAQNRFACVLRVLKDKRRGPTWNHEVLYNGPAGLC"
     gene            complement(2837..>3593)
                     /locus_tag="VFY96_10050"
     CDS             complement(2837..>3593)
                     /locus_tag="VFY96_10050"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5606847.1"
                     /translation="RGTHELILETSRPYVQGDTMHINMIQTLKNALLHGGGVPVRINP
                     GDFAVYESEYNTRAATVLLLDMSWSMSWEGRFAAAKKVALAMESLVRSLHPRDYFGIV
                     GFFTRAVELKAKDLPQATWNMGDPFTNLQDGLHLATEMLGKKPSANQQMIVITDGQPT
                     AYCRQGRLYCEWPLSFGGISQRAAEETLREVERITRKGITINTFMLDDSPVLRGFVDD
                     LTRINKGRAFYTRPDRLGEYLLVDYLAHQRKKV"
BASE COUNT          860 a         1001 c          937 g          795 t
ORIGIN      
        1 cagctagttc ccaccggccc tcggcgcata acccctgtat accggcatcc tcgtacgcgt
       61 gaaggatggc gtcaaggtat gcctcccgga tacgtttggc cagatcttca acattcataa
      121 gctctccaag taactggcaa tagtccaatt ccgacaatga caaattttta ttcgcctaaa
      181 aatttactgg ggaaggcact gtcctgccac agatcggctc tcgacctgga taatctcgcc
      241 ttagtttcac catcgacgga tcgaccgcca aacattagct accgactccg ctgcatgctc
      301 cgtcccttcg gtcaacggaa cggatcctgc ctttcgactg gtaactttta gtcaacatat
      361 aggcatacag agtgaaattg tggagtgcga cgttgcgatt tgcgaaccga tttattcgac
      421 ggctccttgt aaaaaccatt tccgcctgat gcggtcgtaa tagagccata ggagatcgcc
      481 gttgcgggtc tcggcgtaat agtattcgcg ctggatttcg cgattccacc agccgccgca
      541 aaagatatat gggccggcga gcttgtcaac ggcgccggat ttcgcgccca aaagccagcc
      601 gtcttcatgc gatcggagcg gcgcgccgtc aatagttccg ggttttgtgc aaatgcgacg
      661 gatcaaagtt ttgactgtac tcgccttggg tttgggggat tggacccgat ggagaggctc
      721 ccaggtaaat cgcgcttcgg gaaggtggcc gtcattgagt ttagcacaga ctacgctttc
      781 atccccaaat tcagcacgca agcgagccag cgcgcggttt gccgcttcga gattgcggcg
      841 cgacccttcg agttgaccca aaacggagcg ttcgaataaa aaaagctgtt cgcgcgtagc
      901 cgcgcatgat tcgaccgtga gctcgatttc gaccacaccc gcatcgagct gaagcgactc
      961 cagacgtagg cgaataagat cggtgatctg cacggcctcc agcgttggcg acgcgggccg
     1021 cagcgattct ctgattcgat ttctatgatc gatcacgagc gaaatccata gcgtgaccag
     1081 tgcttgatag cgcttcgcca gcgccgcgag cagtggatta agtaattgtt tgatgacaaa
     1141 caaaagccgc gtcgcatcat cttcggggtc atcgagaata tatttttttg ttagcggttc
     1201 ttccggcgat tcgggttcca gcggcatcca gagatcgccg acggccatac gataaagccg
     1261 atgggcttcc ttgccgaacc gctcccgaag tccgccggat ggcagcaaaa gtagatcgct
     1321 gacggttttt attccgagct tgaaaagcgc gtcacgaaac tccggttcga tattcaatcg
     1381 ctgcagcggc gctttgcgtg cagcgtttcg ttccgtctcc ggatcgtgaa aaacagtaac
     1441 gcctcctttc gctttgacgg tggcataagt gccaaaccgc gtgaagccga ccgcgagatt
     1501 caccgcgaat cctcgcctgc gcaaattctg gtaaatggca tgagcccatt gctgcggaga
     1561 ataatagagg tgttgcaggc cggcgccatt gagccaaaaa actcccggct cttcacgcgc
     1621 tggctccact tccggagtaa aacgcatgag cgccttgact aattcaccga cagctttatc
     1681 gatctccgcg ggcaagactt gaccggcgcg aagcgtagaa gcgagggaaa acgctgccgc
     1741 atagcgcaag ccggggagca ctccctgccg gcgtgctttt tcattgaccc aaagcaacaa
     1801 tccttgcggt ttatcttcgt cgacaactgc tgccggataa gcacgccatt ccggatggcg
     1861 gcggagcagc aactgaagcg gaaaagccgg taaatcaaca caacccggcc ggaccgttgt
     1921 agaggacttc atgattccac gtagggccgc gccgtttatc ttttaaaacg cgcaaaacgc
     1981 atgcgaagcg attctgcgcc atgcgttttt tttgggcgtg agcgcggagc gaaaccaatg
     2041 agcccaaaga agaagctgcg cgattttttt tcgtgagaca aaccagcgca gtgtgatgac
     2101 gatgcgcaag gccggtcaag cgcgtctgca aaggcatcgc aatatcggca gcgccgagat
     2161 cgagcacgat taggccgaaa ccaccggagc gcaaaagttt ctcgccagcc cgcgcgatgc
     2221 aggttggctc ggtcaaacga atcaccacca acgcggctaa atcaatccca tttcgcgcgg
     2281 cgtcaggcgg atagaaaaaa tctttcgtcg aggccaccca gcccaccggc tcaccgcgtt
     2341 gttgcgcctc gtcaatcaaa ctgaacgtca atgtcagcgc cgccgatgcc ccagtgctcg
     2401 aaatctcgat aaaccgaccg gcgacttgat cgagccgcca tggagtctcc gacggggaag
     2461 aaaactgcag atctggagcg ggcgaaagga ccatcgatca tacctgtcgt tcaaatcgtc
     2521 taaacggttc aagtcttccg gaagacgttt aaaccgaagg tccggacctc atgtgctttt
     2581 gattttttca tcgccggcat tttacatcat caagtaaatc atcgatcaga atcgataacc
     2641 atttgatatt acttcattct aagcgattct ggtattgcgc agatctcgta tgctagaagt
     2701 acgcttcatt tatactgaaa atatattcag tatacaagcg caaagcaaca cgcacacagc
     2761 tgcccgctgc aatcagctta acggtgtaaa atttgaacgg cttaaaggat cgaacaggca
     2821 atcacgctaa agcgcgctaa actttttttc tttggtgcgc gaggtagtcg acgaggagat
     2881 attctccgag tcgatcggga cgggtgtaaa aggctcggcc cttattgatg cgggtgaggt
     2941 cgtcaacgaa cccgcgcaag accgggctat cgtcgagcat gaacgtgttg atcgtgatac
     3001 ccttgcgcgt gatgcgctca acttctctca aagtttcttc cgcagcgcgc tgactgatgc
     3061 cgccgaagct caacggccac tcgcagtaaa gcctcccctg gcgacaatag gcggtgggct
     3121 gaccatcagt aattacgatc atttgttgat tggcgctagg ctttttccca agcatttccg
     3181 ttgccaagtg taaaccatcc tgaagattgg taaacggatc gcccatattc caggttgcct
     3241 gcggcaaatc cttagcttta agctctacag cacgggtgaa aaatccgacg atgccaaaat
     3301 aatcgcgcgg gtgaagcgag cgaaccaaac tctccatcgc caacgcgact ttcttggccg
     3361 cagcaaaacg cccctcccag ctcatcgacc aactcatatc caagagcagc acggtcgccg
     3421 cgcgcgtgtt gtactccgat tcgtaaaccg cgaaatcgcc cggattgatc cgcaccggca
     3481 cgccgccgcc atgaagcagc gcgttcttca gcgtctgaat catgttgatg tgcatggtat
     3541 cgccctgcac atagggacgg ctggtttcga gaatcaactc atgggtcccg cgg
//
LOCUS       DASULZ010000492         2401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_770190, whole genome shotgun sequence.
ACCESSION   DASULZ010000492 DASULZ010000000
VERSION     DASULZ010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770190"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VFY96_10055"
     CDS             complement(<1..698)
                     /locus_tag="VFY96_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5606848.1"
                     /translation="MISVLLKRFVYGLIVLWAVASLTFILLRLAPGGPFDSERKLPPE
                     IIANLETKYHLNEPVLDQYVRYLAGLARGDLGPSYKYLDRGVTDIIADTLPTSALLGA
                     LAVLFAVLASFPLGLFAAYYRESVIDRLCLFLATLGISLPNFILGALLIWAVALQLGW
                     LQAGRWEDWSSVILPTVTLGAAPAAYLSALLRSSLIETLGEDFVRTARAKGAREHSVI
                     ARHALRNSLIPILTV"
     gene            complement(941..2308)
                     /gene="accC"
                     /locus_tag="VFY96_10060"
     CDS             complement(941..2308)
                     /gene="accC"
                     /locus_tag="VFY96_10060"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009627381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX5606849.1"
                     /translation="MSVTRVLIANRGEIAVRIIRACDSLGIETVAAVSEADRESMAAR
                     LAHRAVCIGPARSTDSYLKVENLIAAAQGTGCDALHPGYGFLSERASLAQACAENRIT
                     FVGPSAENIMQMGDKLEARKIARSAGVPLVPGSDHAKNPHQAVQLAESIGYPLLLKAS
                     AGGGGRGIKLVSNRDEIEETFRTAAAEARAAFGNDTLYMEKYVGNARHIEVQILGDSH
                     GNVIHLGERDCSLQRRHQKIIEEAPAYAVPPEVRAKICNAAATLARNIAYRSAGTIEF
                     IYDNDSAEFYFLEMNTRIQVEHPVTEMITGIDLVAQQLRIARGEALPFRQADIRFTGH
                     AIECRINAESPQHGFRPCPGHIADWQEPRGPGIRVDTHCFSGYFVPPYYDSLLAKLIV
                     HADTRQDAANRTFHALDHFHVSGIDTLIPFLQIVIDDPEYRAGAVNTRWLEKKLEQYS
                     AGHSG"
BASE COUNT          567 a          655 c          698 g          481 t
ORIGIN      
        1 acggtcagaa tagggatgag tgaattgcgc agcgcatgtc gtgcgatcac cgaatgttcg
       61 cgcgcgcctt tggcgcgtgc ggtccgcaca aaatcttcgc ccagcgtttc gatcagactc
      121 gaccgtagca gcgcggatag gtaggctgcc ggcgcggcgc ctagcgtgac cgtcggcaga
      181 atcacactgc tccaatcctc ccagcgaccg gcttgcagcc agcccaactg caaagctacg
      241 gcccagatga gcaaagcgcc gaggatgaaa ttcggcaacg agatgccgag agtggcgagg
      301 aataaacaca agcgatcgat gaccgactcg cgatagtacg cggcgaatag ccccagggga
      361 aaagatgcga gcactgcgaa gagcacggcc agagcgccga gcaaggccga agtcggcaag
      421 gtatcagcga taatgtccgt gacgccgcga tcaaggtact tataagaggg accaagatcg
      481 ccgcgtgcca atccggcgag ataccgaaca tattggtcga gcaccggctc gttcagatga
      541 tatttggttt cgagattggc gatgatttcc ggcggcagct tgcgttcgct atcgaagggc
      601 ccgccgggcg caagccgcaa aagaatgaac gtcaggctgg ccaccgccca gagaacgatc
      661 aagccataaa cgaagcgttt cagcagtaca gaaatcatct agctgtgctg aatgtaacgt
      721 agagtttctg gcttgtcatg cgaccgtggc ggaatttggt cgcaagcagg aaaatcctca
      781 cgaagtgcac gaaggacacg ccgatcctga gagactcgga actggaaagt ggtcgttctg
      841 agcgtgaaaa agaatctcgc tccattccag cttatgagac gagatccttc ggtagcaggg
      901 atgacattcc ccacactctc tgagcaattt tactgcaggg ctaacctgaa tgacccgcgg
      961 aatattgttc cagctttttt tccagccacc gcgtattgac ggcacccgcg cggtattcgg
     1021 gatcgtcgat gacgatttgt agaaagggaa tcaaagtgtc gatgccgctc acgtgaaagt
     1081 gatcgagcgc gtgaaatgta cgattcgcag cgtcctggcg tgtatcggcg tgtacgatga
     1141 gcttggcaag cagagagtcg taataaggtg gaacgaagta gccggaaaaa caatgagtgt
     1201 ccacgcgaat tcctggtccg cgcggctcct gccaatcggc gatatggccc ggacagggac
     1261 gaaatccatg ctgcggcgat tccgcgttga tgcggcattc aatggcgtgg ccggtaaatc
     1321 ggatatcggc ttgccggaat ggtagcgcct cgccgcgcgc gatgcgtaac tgttgggcga
     1381 ccaaatcgat gcccgtgatc atttcggtca ccgggtgctc gacctgaatg cgggtattca
     1441 tctcgagaaa ataaaattct gcgctgtcgt tatcgtaaat aaactcaatc gtgcccgcac
     1501 ttcggtaggc gatgttccgg gccagggtcg ccgctgcatt gcagattttc gcgcgcactt
     1561 ctggcggcac cgcatacgca ggcgcttctt cgataatctt ttgatggcgc cgctgtaaag
     1621 aacaatcgcg ctcgccgaga tggatgacat taccatgtga atcaccgaga atctggactt
     1681 cgatatgacg ggcgttgccg acatattttt ccatgtaaag cgtatcgttt ccgaacgcgg
     1741 cccgcgcttc ggcggcggcg gtgcgaaaag tctcttcaat ttcgtctctg ttcgacacca
     1801 acttgatgcc acggccaccg ccgcccgcag aggctttcaa caacaacgga tagccaatcg
     1861 attccgccag ctgcacggct tgatgcggat tttttgcgtg atcggagcct ggcacgagcg
     1921 gtacaccggc agaccgggca attttgcggg cttcgagctt atcgcccatc tgcatgatat
     1981 tttccgcact gggaccgacg aaggtaatgc gattttcagc gcaggcttgc gctaggctgg
     2041 ctcgctccga aagaaaacca taacccggat gaagcgcgtc acagccggtg ccctgcgcgg
     2101 cggcgatcaa gttctccact ttcaaatagc tgtcggtcga gcgcgccggt ccgatgcaca
     2161 cggcccggtg cgccagccgg gccgccatgc tttcgcggtc cgcttctgaa accgcggcaa
     2221 cggtttcgat gccgaggcta tcgcaggcgc ggataatgcg aacggcaatc tcgccgcgat
     2281 tggcgatcaa cactcgggta accgacatca acgctcctga ccccaagaaa aagggacaag
     2341 taactttccg aagagcggaa aagtaactcg tctcatatcg agaagaaaaa gatctacggt
     2401 t
//
LOCUS       DASULZ010000493         2671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1277862, whole genome shotgun sequence.
ACCESSION   DASULZ010000493 DASULZ010000000
VERSION     DASULZ010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277862"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VFY96_10065"
     CDS             complement(<1..434)
                     /locus_tag="VFY96_10065"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5606850.1"
                     /translation="MDLRVISANFGFLIVQGFIGLGAFAGGTLRLAIPAIFLGFILGT
                     LIGLARLARSRWIYMPAAVYVEFFRGVPLVMVIFWFWFMIPTLVGKSLPEYSVALTAF
                     VVFEAAYLAEIVRSGIQSVPRGQSEAAIATGLTRQQTMRLVI"
     gene            complement(440..1162)
                     /locus_tag="VFY96_10070"
     CDS             complement(440..1162)
                     /locus_tag="VFY96_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020880647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX5606851.1"
                     /translation="MTNRLSWNILWSGQTGGWLLQGVITTLEISVIAWLLALALGLLS
                     GTLRTAPALPLRWIGAAYVEFFRNVPLLVWMFFWYFAVPPLLPQVLQDWLLEHGAEFW
                     AGVVALGVYHGARFSEVIRSGIQAIPKTQFEAALSTGLTMVQTYRLIIIPVALRLIVP
                     PATNETLNLLKNSSVALTIGVAELTFQTRQIETYTAKAFEALTAGTIIYLVLCLIIAL
                     LMSQIEQRTAIPGMIARGTGEF"
     gene            complement(1239..2099)
                     /locus_tag="VFY96_10075"
     CDS             complement(1239..2099)
                     /locus_tag="VFY96_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX5606852.1"
                     /translation="MILARCVSVWVISILLASFGFVSAQPTLEKIDKSGVLIIGTRTG
                     SPPFAYVNKNNEWVGFSIELVEKAVVPGLAKKLNKQIRLEKKESAPQTRIPLLTSNAV
                     DLIAETMTDTQSRRDQVDFSLTFFVTGAQFLVKKGTPIKNIQSVAGKRIAAQQGSTNA
                     KIIRERVPTAKLLEFPDQPAAFQALAQGQVQAYTNDGVQLAGLRAKAPRPDEWNVVGE
                     FFSYEPYGMALRKNDSDFRQAVNVALMDAISSGLYFQLYDKWFGVKGEVPYPLSAETK
                     RFLEMQVVPK"
     gene            complement(2108..>2671)
                     /locus_tag="VFY96_10080"
     CDS             complement(2108..>2671)
                     /locus_tag="VFY96_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014320805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX5606853.1"
                     /translation="GSLTVDGLSLADAQLDSVRLRAKVGMVFQSFNLYPHMSVLRNIT
                     LAPIQVKGLAAAEAKKIAIDLLERVGIPDKADAFPAHLSGGQQQRVAIARALAMQPKI
                     MLFDEPTSALDPEMINEVLEVMTGLARDGMTMMVVSHEMGFARRVAHRIVFMDQGRIV
                     ETGEPANFFAAPQSPRAQQFLSKILSH"
BASE COUNT          674 a          724 c          718 g          555 t
ORIGIN      
        1 aaaatcacaa gccgcattgt ctgctggcgc gtgagaccgg tcgcgatggc tgcctcgctt
       61 tgaccgcgcg gcaccgattg tatcccggag cggacgatct cagccaaata agccgcttcg
      121 aatacgacga acgcggtaag cgcgacgcta tactcaggca gcgatttgcc gacaagtgta
      181 ggaatcatga accaaaacca aaaaatcacc atgacgagcg gcacgccgcg aaagaactcg
      241 acgtaaaccg ccgccggcat atagatccaa cgcgaacgtg ccaaacgtgc gaggccaatg
      301 agcgttccaa gtatgaatcc taaaaaaatc gctggaatcg caaggcgcag ggtgccaccg
      361 gcgaaagcgc ctaagccaat aaaaccttgg acaatcagaa agccgaagtt ggcgctgatt
      421 acccggagat ccatatggtt tagaattctc ctgtgccacg cgcgatcatg ccgggaattg
      481 ccgtgcgttg ttcgatctgc gacatcaata aagcaatgat caggcagagg acgagataga
      541 taatcgttcc tgccgtgagc gcttcgaatg ccttggcggt ataggtttcg atttggcggg
      601 tttgaaacgt caactcggct acaccgatgg tcaatgccac ggatgaattt ttcagcaggt
      661 tgagcgtttc gtttgtcgca ggaggcacga tcagacgcag ggcaaccgga ataatgatca
      721 atcggtaagt ctgtaccatc gtaagcccag tcgatagcgc cgcttcaaat tgcgtctttg
      781 gaattgcctg gatcccagaa cggatcacct cggaaaagcg cgcgccgtga tatacgccga
      841 gcgctaccac tccggcccaa aactcagcgc cgtgctcgag cagccaatcc tgcagcactt
      901 gcggcaataa aggtggcacc gcgaaatacc agaaaaacat ccagacaagc agagggacgt
      961 tgcggaaaaa ttcgacgtag gcggcgccga tccatcgtag aggcaaagcc ggcgccgtgc
     1021 gtagagtgcc cgaaagtaaa ccaagcgcga gcgcaagcag ccatgcaatg acggaaatct
     1081 ccagggtcgt tatgacaccc tggagcaacc agccgccggt ttgaccgctc cataggatgt
     1141 tccaactgag gcgattggtc attttgacag acgcacgaga aacgaggcga aggacacgag
     1201 cggaaaacgg atcctgaaaa tcggcctcat gcctgagctt atttgggcac gacttgcatt
     1261 tccaagaaac gcttggtttc agcggagagc ggatagggca cttccccttt gacgccgaac
     1321 catttgtcgt acagctgaaa gtagagaccg gaggagatcg cgtccatcaa ggcgacgttc
     1381 accgcttgac gaaaatccga gtcgtttttt cgcagggcca taccgtaggg ttcataagaa
     1441 aaaaattcac ccaccacatt ccattcatcg ggtctcggtg cttttgcccg caagccggcg
     1501 agctgaactc cgtcgttcgt gtaagcctgc acctgtccct gcgccaaggc ttgaaatgcg
     1561 gcgggttgat cggggaattc aagtaacttc gcagtgggaa cacgctcgcg aataattttt
     1621 gcgttggtcg atccttgttg agcggcgatt cgtttgcccg caacgctctg aatgtttttg
     1681 atcggcgtgc ctttcttcac gaggaattgt gcgccggtga caaagaacgt taagctaaaa
     1741 tcaacttgat cacgccggga ctgcgtatcc gtcatagttt cggcgatgag atccaccgca
     1801 ttggatgtga gtaacggaat ccgggtctgc ggcgccgatt cttttttttc cagcctaatt
     1861 tgcttgttga gctttttggc cagcccggga acgaccgcct tctccaccag ctcgatcgaa
     1921 aagccgaccc attcgttgtt tttattgaca taggcaaagg ggggtgaccc cgtccgggta
     1981 ccaatgataa gaacaccgct cttatcaatt ttctcgagag tcggttgagc gctgacaaaa
     2041 ccaaaagatg ccagcaaaat cgaaatgacc caaacgctga cgcatcgagc gagtatcatg
     2101 tcaactccta atgagaaaga atcttgctta agaactgttg agcgcgcggc gattgaggcg
     2161 cggcgaagaa attcgcgggc tctccggttt caacgatgcg tccctgatcc ataaacacga
     2221 tgcggtgcgc gacacgccgc gcaaaaccca tctcgtggct gacgaccatc atcgtcatgc
     2281 catcgcgcgc gagcccggtc atcacttcga gaacctcgtt gatcatctca ggatccagtg
     2341 ccgaggtcgg ctcatcgaac agcataattt tcggctgcat cgctagcgct cgcgcgatcg
     2401 ccacgcgctg ctgctgtccg ccggaaagat gcgccggaaa ggcatctgct ttatcaggaa
     2461 tgccaactcg ctccaaaaga tctatggcaa ttttttttgc ttcggctgcc gcaagccctt
     2521 tcacttggat cggcgccagg gtgatattac gcagcacgct catgtgcgga tacagattga
     2581 aagactggaa caccatgccg accttggcgc gcagccgtac cgagtcgagt tgtgcatccg
     2641 ctaaagacag accgtcaaca gtaagagaac c
//
LOCUS       DASULZ010000494         4179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1277863, whole genome shotgun sequence.
ACCESSION   DASULZ010000494 DASULZ010000000
VERSION     DASULZ010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277863"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VFY96_10085"
     CDS             <1..310
                     /locus_tag="VFY96_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014870585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606854.1"
                     /translation="VPNAIDLAKSERSKWLLRVAVIGPNDINRLFTLPAGVPAERVAA
                     MRKAFDATFADPEFQADIEKARVVLRRIPIERIQEVSLLWLDMPAADKKELQKILKIS
                     "
     gene            complement(537..1862)
                     /gene="ligD"
                     /locus_tag="VFY96_10090"
     CDS             complement(537..1862)
                     /gene="ligD"
                     /locus_tag="VFY96_10090"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX5606855.1"
                     /translation="MTKRDTAEVLSVEGREVRITHPDKPYFSKETKASKLDLVRYYLS
                     VAPGALAGIRDRPLVLKRFVDGAEGQAFYQKRAPANVPPWLRTVTLSFPSGRKAEEIV
                     VDDAAGLAWVVNLGCIELHPHPVRSRDLDHPDELRVDLDPGPGVAWDDVRRVALEVKS
                     FLEEMGLRGWPKTSGSRGMHVNVRIEPRWTFTDVRRAALALSRAIERHAPALASSKWW
                     KEERHGVFLDYNQNAKDRTTCSVYSVRPLPDARVSTPLHWDEVPDCDPADFTLLTVPK
                     RFAELGDPHRDMDSAPGSLEKLLELAAEDEAAGLGDAPWPPHFKKMEGEAPRVAPSRA
                     RATSKRAVAKKSRSKAPLLVVANSPDKQAALAGLERWKLKYPQAAARLAVDDVLVDSM
                     RGRSSTWTRIRVNLRNVPEKLRPPQETPDPDEDPTRAWRARHVAQHDRS"
     gene            1947..2249
                     /locus_tag="VFY96_10095"
     CDS             1947..2249
                     /locus_tag="VFY96_10095"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX5606856.1"
                     /translation="MRVEAERGRESFLPDRFYFPKAKSAALTRFPSGKKNPTGNERND
                     HHALAITLAAGAGSFVTIEGGFPIFADGEIVGAVGVSGAGIKDIDISQTAAAVLGK"
     gene            2785..3009
                     /locus_tag="VFY96_10100"
     CDS             2785..3009
                     /locus_tag="VFY96_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606857.1"
                     /translation="MGLFLLAGLAGIALHFQSSMEFRLETNPSLNGWALFWAAISAKT
                     PPALAPGAMVQLGLLGLVYTYRYPSADAQN"
     gene            3037..3675
                     /locus_tag="VFY96_10105"
     CDS             3037..3675
                     /locus_tag="VFY96_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606858.1"
                     /translation="MKNSVILSILFAGWVLAALAPLNAQVGRHQEMIEPNVADEKELA
                     KLPHLNRELVKGIVERRPFANMLELHKFLSSSLDSKQLNELYSKMFIQINLNTASREE
                     IMLIPGMGNRMVREFLEYRPYTAMAQFRKEIGKYVDEKEVARLEQYVFVPINLNTASD
                     ETLATIPGATPQVIRVVKDARPYKDMEQFRREIGKSVSAKEAGRLEHYFVIK"
     gene            3827..>4179
                     /locus_tag="VFY96_10110"
     CDS             3827..>4179
                     /locus_tag="VFY96_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606859.1"
                     /translation="MNTGTPPFRADHVGSLLRPPELLRAREQRQQGSISAEGLRAFED
                     RAIREVAKLQEEIGLQGITDGEYRRTIWHADFCDRLKALPSRKALPTPRALRAGFNRV
                     VRKSNAHRRAFMRRGG"
BASE COUNT          900 a         1151 c         1220 g          908 t
ORIGIN      
        1 cgtgcccaat gctatcgatt tggccaagag cgagcggagc aaatggctgc tgagagtcgc
       61 agtgattggt cccaacgaca tcaaccggct gttcaccctt cctgccggcg ttccggctga
      121 gcgggtcgca gcgatgcgca aagcgttcga tgcaaccttt gccgatcccg agtttcaggc
      181 agatatcgaa aaagcacggg tggtgctccg tcgaataccc atcgagcgca tccaggaggt
      241 ttcgttgctg tggctggata tgcccgcggc ggataagaag gaactgcaaa agattcttaa
      301 aatcagttga actaaaagga caacagacaa gcgggtttgg tcaaagatag ctactgtgct
      361 ggaattcaaa ttcattcaac aaatcatccc atgttttgcc gtcataccgg cccttcgtta
      421 tgctcaggat gaactccagc cggtatccag atgtagttga atttcctgga ttgcgggtcg
      481 cgctagctaa cgccagcttg cccggaatga cgatcactca gaacggacca gcggcgttag
      541 ctccggtcgt gctgcgccac gtgccgggcg cgccatgcgc gcgtcgggtc ttcatcgggg
      601 tcaggggttt cctgcggcgg gcgcaatttt tccggcacgt ttctgagatt tactcggata
      661 cgcgtccaag tggacgatct gccgcgcatg gaatcgacca gcacgtcatc tacggccagt
      721 cgtgcggcgg cttgtgggta tttcagcttc catctttcca ggccggcgag tgccgcctgc
      781 ttatcggggg aattcgcgac gaccagaagc ggcgccttcg atcgactttt tttggcgacg
      841 gcccgcttcg aagttgcgcg ggcacgcgat ggcgccaccc gcggcgcttc gccctccatc
      901 ttcttaaaat gcggtggcca cggcgcatcg ccaagacctg ctgcctcatc ctcggcggcc
      961 agttctagaa gcttttccaa cgagcccggg gccgagtcca tgtctcggtg gggatcgcct
     1021 aactcggcga accgcttcgg aaccgtcagc agggtaaagt cggcgggatc gcagtccggc
     1081 acctcatccc agtgaagcgg cgtggagacc cgcgcgtcgg gaagcgggcg gacagaataa
     1141 acagagcagg tggtgcgatc cttggcgttt tggttgtagt ccaggaagac gccgtggcgc
     1201 tcctccttcc accatttcga gctggccagg gccggcgcgt gccgctcgat ggcgcgcgat
     1261 aaagctagag cggcccggcg cacgtcagta aacgtccacc gcggttcaat ccgaacattc
     1321 acatgcatgc cgcgcgagcc gctggttttc ggccagccgc gcagtcccat ctcctccaaa
     1381 aatgatttca cttcgagcgc cacgcggcgc acatcgtccc acgcgacgcc gggtccagga
     1441 tcgaggtcga cgcgcaactc gtccggatgg tccaggtcgc gcgagcgcac aggatgagga
     1501 tgaagctcga tgcaacccag attgacgacc caagcgagcc cagctgcgtc gtcgacgaca
     1561 atctcctccg ctttgcgccc tgaaggaaat gatagtgtaa ccgtgcgcag ccatgggggg
     1621 acatttgcgg gcgcgcgttt ctgatagaag gcttgccctt cggcgccatc gacgaaccgc
     1681 tttagcacca atgggcgatc acggatgcca gcgagagcgc cgggcgcaac cgagagataa
     1741 taacgcacga ggtcgagctt cgaggccttc gtctctttgg aaaaatacgg cttgtcggga
     1801 tgggtaatgc ggacttcgcg tccttcgacc gagagaactt cggctgtgtc ccttttagtc
     1861 acagagcact gatcgtttgt gcgaatcgct caaccgaatg aactctaaca gttcgggcga
     1921 gtcagtctag tgttctgact cgtggcatgc gagtggaggc cgaaaggggt cgggaatctt
     1981 ttcttcccga tcgcttctat tttcccaagg cgaagagtgc ggcgcttacg cgattccctt
     2041 cgggcaaaaa aaatcccacg ggcaacgaac gcaatgacca tcacgcgctt gccattacac
     2101 tcgccgcggg cgctgggtcg tttgtcacga tcgaaggggg atttccgatc tttgccgacg
     2161 gggaaatcgt cggcgcggtg ggtgtcagcg gcgcgggaat taaggacatc gatatctcgc
     2221 agaccgcggc cgcggtattg ggcaaataag ccgcgcaaat cgttgttgcg cttcggtccc
     2281 gcggattcag caatgagcga cagccgtcag aaatatttac tgccgtgttt tggcctgctg
     2341 gaagaatttc agcgcgtccg gctccgcagg acggctggcg caacaactcc tgccgtagtc
     2401 gttgctgtcg aagtgcaacg ccggcacgag aatccgtagt tcgataaagg ttcgtaaaca
     2461 ccaccgttcg aataccgcga gacgctgaga gaacactcgg caacttacat tatcggacat
     2521 cctcgggtgt gctgacttga tacgagtaca cccaagatgt aatttgtcgg caatttgtcg
     2581 ccaccagagc acatcgttcg ttcactcctg ctgggagtgc tgtttgttga tttattcgga
     2641 acgggcgcgg agcttctact gctacatcat actaagagtc tctgggaact gatcccgctt
     2701 gtgctgattg gtctggctct catcatcctg acgtggcatg cagttgcaca gcacccggca
     2761 agcgttggct tggtccgagg cgtcatgggt ctttttctgc tagccggtct ggccggcatc
     2821 gcccttcatt ttcaaagcag tatggaattc aggctggaaa ccaacccgtc cttgaatgga
     2881 tgggcactgt tttgggcagc aatttctgcg aaaacaccgc cggccctggc accgggtgcg
     2941 atggttcagc tggggctgct cgggcttgtg tacacgtatc gatatccgtc tgcggacgca
     3001 caaaattgac gaaccataga aaggaaaatg agcagcatga aaaactctgt tatcttaagc
     3061 attctttttg ccggctgggt gttggctgcg ctcgcacccc tgaacgctca ggtcgggcgc
     3121 caccaggaga tgatcgagcc taacgtcgcc gatgaaaaag aactcgccaa gctgccgcac
     3181 ctcaaccgcg agcttgtgaa aggaatcgtg gagcgacgtc ccttcgcgaa tatgctggag
     3241 ctgcacaaat ttctcagttc atcgctcgat agcaaacagc taaacgagct ttattcgaaa
     3301 atgttcattc agattaatct taacacggcg tcacgcgaag agatcatgct cattcctggc
     3361 atgggaaacc gtatggtccg cgagttcctc gagtatcgac cctataccgc gatggctcag
     3421 tttcgtaaag agatcgggaa atacgtcgac gaaaaggaag tggcccggtt ggaacagtat
     3481 gtatttgtgc cgatcaattt gaatacggct tcggatgaaa cgctagcgac tattccaggc
     3541 gctacgccgc aagtcatccg ggtggtcaaa gatgcgcgac cctataagga catggaacaa
     3601 ttccgtcggg aaatcggaaa atccgtgagt gctaaggagg ccgggaggct cgaacattat
     3661 ttcgtgatca aatgagtttc gcgccttcgg tgcagcgtgc tgttcaacgg attgatcaaa
     3721 gcattgtgca actttacgtt tattctcatg gctcttctcg tcacaaatca aaggtggcaa
     3781 tgatcagagc gattgttttc agaatcttta gcgaaaggca ccgccaatga acactgggac
     3841 acctcctttt cgcgccgatc atgtgggcag cttgcttcgg ccgccggaac ttttacgcgc
     3901 gcgcgagcag cgtcagcaag gaagtatctc tgccgagggg ctgcgcgcat tcgaagaccg
     3961 cgctattcgc gaggtcgcca aactccagga agagatcgga ttgcaaggca tcaccgacgg
     4021 cgagtatcgc cgcacgatct ggcatgcgga tttttgcgac agattgaagg cgttaccgtc
     4081 caggaaggcg ttgccgacgc cgcgggcctt gcgcgcaggt ttcaatcggg tggtcaggaa
     4141 atcgaacgct caccgacgcg cttttatgcg acggggcgg
//
LOCUS       DASULZ010000495         2440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_770310, whole genome shotgun sequence.
ACCESSION   DASULZ010000495 DASULZ010000000
VERSION     DASULZ010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770310"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VFY96_10115"
     CDS             complement(<1..214)
                     /locus_tag="VFY96_10115"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX5606860.1"
                     /translation="MDLLKQTSLVFWKDLLLELRRRDSLLTMFFFGTLLLFVFHFSFD
                     LPPDKVAEMAPALLWLAFLFTGTLGLT"
     gene            complement(202..1002)
                     /locus_tag="VFY96_10120"
     CDS             complement(202..1002)
                     /locus_tag="VFY96_10120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5606861.1"
                     /translation="MRAALESAIAGQYSRCTQNLVQLQSAIGPKLRLSVDRLSKAYNF
                     LWALRDLSVELEPGDLVALLGPNGAGKTTLLKLLVGLIAPTSGSIRFDGTSRLAPLAR
                     PRTGLLMPADHLYDNLTVRENLSFFLSLYGDRCDLQMIDSALDAVGLLERGAEFVGNL
                     SSGMRCRASLAKWQLLQPDLLLLDEPYGVLDGSGIDLLEKFLRHQVANGGIVVMASHH
                     VSRALKLCSRALILKQGRLTFNEVKRQPWPSFDRAFGEFLPEGERWIS"
     gene            1083..1766
                     /locus_tag="VFY96_10125"
     CDS             1083..1766
                     /locus_tag="VFY96_10125"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606862.1"
                     /translation="MNPIVTVTGFWDAFVTKIAVFLPNLFFSIAILLLGWVICNVIKR
                     IVVRVLKLCQFDALAERAGIKQILERGGIRQAPSDIIGLLTFWFLFLIAIVTTLETLN
                     LSGATDTLHTIYLYIPKIVAALVTLSLGLYFANFLETVTRTSCANAGLHAAASIGRAA
                     YIGTTLFVVAGIFEILDIASEIVIWAFILVFGAVCLALALAFGLGGRDVAARYLERWL
                     EQKKIEKIE"
     gene            1894..>2440
                     /locus_tag="VFY96_10130"
     CDS             1894..>2440
                     /locus_tag="VFY96_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006039749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX5606863.1"
                     /translation="MAEYKVVKVPKDGAKITMGANKQLQVPDNPIIPFIEGDGTGRDI
                     WRASVRVFDSAVEKAYKGKKKIRWMEVYAGEKSFKQYNSWLHEETVPAFSEFLVGIKG
                     PLTTPIGGGMTSLNVGLRKLLDLYVCQRPVRYFNGVPSPVKHPEYVDMIVFRENTEDI
                     YTGIEFPNGTEANAKFKSLVKD"
BASE COUNT          577 a          631 c          686 g          546 t
ORIGIN      
        1 gcgttagccc gagcgtgccg gtgaaaagga acgcgagcca gagcagtgcg ggcgccattt
       61 cggcgacttt gtcgggaggg agatcgaacg aaaaatgaaa cacgaacagg agcagcgtcc
      121 cgaaaaaaaa catcgtgagc aaactgtcgc ggcgccgcaa ctccagcagg agatctttcc
      181 agaacacgag cgaggtttgc tttaagagat ccatcgctct ccctctggta aaaattcccc
      241 gaaagcgcga tcgaagctgg gccaaggctg tcgcttgact tcgttgaagg tgaggcgacc
      301 ctgtttcaag atgagcgcgc gactacaaag tttaagcgcg cgcgagacgt gatgcgaagc
      361 catgacgaca ataccgccgt tcgcaacctg gtggcgaaga aatttctcca gcagatcgat
      421 accgctgccg tccagaaccc cataaggttc atcgagcagc agcaggtcgg gctggagaag
      481 ctgccatttt gcgagtgagg ctcggcagcg cattccactg gaaagattgc cgacgaattc
      541 cgcgccgcgc tcgagcagcc cgactgcgtc cagcgccgaa tcgatcatct gcaaatcaca
      601 tcgatcgccg tacagcgaga gaaaaaaact aagattctcc cggacagtta aattgtcgta
      661 caggtgatcg gcgggcatca aaagcccggt tctcggccgt gcaagcggcg cgagccgcga
      721 tgttccatcg aagcggatcg agcctgaagt cggggcaatc aggccgacaa ggagttttag
      781 cagtgttgtt ttgcctgcgc cgttgggacc cagtaaggcg accaaatcgc ccggctcgag
      841 ttccacgctc aaatctcgga gcgcccagag aaaattgtag gctttggaca atctatcaac
      901 ggagaggcgc aatttaggtc cgatcgcaga ctggagttgt acgaggtttt gagtgcaccg
      961 actatattga ccggcaatcg cggattcaag cgccgcccgc acggtggcgg cgctagtttg
     1021 aattatttca ggaagtcccc tatttaggag ttgcgctggt cggttgcacc aaggaggagt
     1081 gaatgaatcc gatcgttacc gtcaccgggt tttgggatgc ttttgtgaca aagattgcag
     1141 tttttctccc gaatctattt ttctcgattg cgattcttct gcttggctgg gtcatttgca
     1201 acgtcatcaa acgaatcgtc gtgcgtgttc tgaagctctg tcagttcgat gccctggcgg
     1261 agcgcgctgg aatcaagcag attctcgagc gcggcggcat caggcaagcg ccgtcggaca
     1321 ttatcggttt gttgacattc tggttcctgt tcttgatcgc gattgtgacc actttggaga
     1381 ccctcaattt gagcggcgcg accgacacct tgcacaccat ctatctttat attcccaaaa
     1441 tcgttgctgc gctggtgact ctcagtctcg gactttactt tgccaatttc ctggaaacgg
     1501 tcacgcggac ttcgtgcgcc aatgctggcc tgcatgcggc ggcatccatc gggcgagccg
     1561 catatatcgg tacgaccctg tttgtggttg cggggatctt cgagattttg gatatcgcgt
     1621 cggagatcgt gatctgggca ttcattttgg ttttcggcgc ggtctgtttg gccttggccc
     1681 tcgccttcgg tttaggcggc cgagacgttg ccgcacgcta cctcgaaaga tggctggagc
     1741 aaaagaaaat cgagaaaatc gaatagttag agtaacttga ctccccgcaa gcaatcagct
     1801 agtttcgatc agttcaaaaa atttttttac gcgaatagcc taaggggtga gtttcacgga
     1861 agcccttagg ctatttttgc aggtgaggag aacatggccg agtacaaagt cgtaaaggtc
     1921 ccgaaggacg gtgctaaaat caccatggga gccaacaagc agctccaggt tcccgacaac
     1981 ccgattattc cgttcatcga aggggacgga acaggccgag atatctggcg cgcatcagtg
     2041 cgggtcttcg attcagccgt ggaaaaggcc tacaagggca aaaagaaaat tcgctggatg
     2101 gaggtctacg ccggggaaaa gtctttcaaa cagtataata gctggttgca tgaagagacg
     2161 gtaccggcat tttcggaatt tctcgtcggc atcaaaggtc cgctcaccac gccgatcggc
     2221 ggcgggatga cctcgctcaa cgtcggcctg cgcaagctgc tcgatcttta cgtctgtcaa
     2281 cggccggtgc gctatttcaa cggcgtgccc tcgccggtca agcatcccga gtacgtcgac
     2341 atgatcgtat tccgggaaaa caccgaggat atttacaccg gcatcgaatt tcctaacggc
     2401 accgaagcca atgccaaatt taaatccctg gtcaaagata
//
LOCUS       DASULZ010000496         3128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1073992, whole genome shotgun sequence.
ACCESSION   DASULZ010000496 DASULZ010000000
VERSION     DASULZ010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073992"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VFY96_10135"
     CDS             <1..187
                     /locus_tag="VFY96_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606864.1"
                     /translation="KMLAIGDRVKVTLRPETGRFPNPTEGKITQKDDAGNFSLQTGQG
                     VIQIAASDILSVTRLSS"
     gene            359..967
                     /locus_tag="VFY96_10140"
     CDS             359..967
                     /locus_tag="VFY96_10140"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX5606865.1"
                     /translation="MVSQLGLIACSSQTTIPTVSDDRVEALVRSEAARIISVTDDKQH
                     LSEYYIFLSDFPRKDVLGMSVGNRRIYISHELAKLASRRPSHLWLLRQTLAHEIAHET
                     AGHARQTSGASLARGPFARSVSNTDIGLPARVTLRNYSAEKEFEADAKGLQYWTRLGW
                     DCRIWVRILQSFEKQNYIGDALHPTDERLRRARAICRTDDAV"
     gene            complement(1427..2542)
                     /locus_tag="VFY96_10145"
     CDS             complement(1427..2542)
                     /locus_tag="VFY96_10145"
                     /inference="COORDINATES: protein motif:HMM:NF013641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX5606866.1"
                     /translation="MTRSADLSAIQNCISNSLGELTLSESTITGVVGDAPSQYSRSPS
                     IWQAAFAELGIEAVYLPFDTSDDQLPHLLTVLRQCKRFWGANITVPHKLRIMEFLDEI
                     DPGALRIDAVNTIVRTQAGRLIGYNTDGEGFVQSILQPSADRSEPFISSLAGMNVLLI
                     GAGGSARAVAFHIADLLVGGKLLISNRTLPTALALADNLRQQGHQAFAISEAQIGKDL
                     LEVGLIINSTTKGQGGIRQLSGGRVTNLEPYSALAPAHPPAFPLSELDTAGFAGAWLQ
                     SARPDIETNHEVSFRLANAIPRHVRFYDLIYHPEETVFLRHGRLTGHLCMNGKGMIIH
                     QAAIAFYQYICREHLKSRGIEGVAAQKQILNAMERAW"
     gene            complement(2605..>3128)
                     /locus_tag="VFY96_10150"
     CDS             complement(2605..>3128)
                     /locus_tag="VFY96_10150"
                     /inference="COORDINATES: protein motif:HMM:NF016201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEX5606867.1"
                     /translation="HVGMKVWEDIEQRWDHPSAEDLENFGPRKKTGREKLFEVREVER
                     DASFLRRYLTEDLIRELNLFEYKSRGNEQVVSRVADEENWRQIKETLIQNVGTGTIPV
                     IKVEDADYHNNRALLLKHDHDGRDLQLEYAEKTLRYLYQLWGRAVAMETLLDGQRTLL
                     NFVDGKFSIKKPA"
BASE COUNT          683 a          831 c          873 g          741 t
ORIGIN      
        1 aaaaatgttg gcgatcggcg atcgggtcaa ggtgacactt cgtccggaga ccgggcgatt
       61 tccgaacccg accgaaggga agattactca aaaagacgat gcaggaaatt tttccctcca
      121 aacggggcag ggcgtcatcc agatcgctgc cagcgacatt ctttccgtta cccggcttag
      181 ttcctaacgg gttcttgctc cattgatttg ctcggcgcgg gcggcaattt gcggccgcac
      241 tgccgctagt gctctcaact gggatgtgct aggttacggc aattttgaca gagagaaggg
      301 cgaccaagct ttgcgcaaat gtaaaacgtg gcgaacgatc acttttttat tcgggctcat
      361 ggtttcgcaa ttaggcttga tcgcgtgctc gtcccaaacg accatcccca ccgtctccga
      421 tgaccgcgtc gaagccttgg tacgaagcga ggcggcgcga attatctcgg tcaccgacga
      481 caagcagcac ctgtcggagt attacatctt tctttccgat tttccgcgaa aagacgtgct
      541 cggcatgagt gttgggaatc gccgcattta catcagccac gaactcgcga aactcgcgtc
      601 gagacgacct tctcatcttt ggctgctgcg tcaaacattg gcccacgaga ttgcccatga
      661 aactgccggg catgcgcggc aaactagcgg tgcgtcactt gcacgtggtc cgttcgcgcg
      721 gtcggtgtcg aacacggaca tcggccttcc cgctcgtgtc acattacgca actattccgc
      781 tgaaaaagaa ttcgaggcag acgccaaggg tttgcagtac tggacgaggc ttggttggga
      841 ttgtcggatt tgggttcgta tactgcaaag ttttgaaaag caaaattata tcggcgatgc
      901 tcttcatccg accgacgagc gcttgcggcg agccagggcg atttgtcgaa cggacgatgc
      961 ggtgtgattg tgactaaatt acgccagcgg tcctttgctt ctttcgtcga ttttctttgc
     1021 tatcttatta attcgtgaat gcctgcactc atgccactct ggagcttttg ccagtcagaa
     1081 aaccgacgat gcgctgccgc cattgtcacc tgacaatcaa tcgggaggac ttaggcgacg
     1141 gttgttgtcc ggaatgcctg gagaaatttg gcaagaggca ctacgatttc gaagaattgg
     1201 aaagtgccag cagcgccagc gtccggtacc gctgtgagga ttgcggcgcg atcatcgaga
     1261 gcgcataatc tgcttccaaa acgcaattca tgaatctccc caacacctca atgcgggaaa
     1321 gattccttac tcttcgtcaa gctgtgaaaa cttctcgtga cgccggtctg tgatgtcgag
     1381 ggtcaactag gtacagtcga gccgtcgcgt ctcgttatta ttgctatcac caggcacgct
     1441 ccatcgcgtt gagtatttgt ttttgagcag ccacgccttc gatgccgcgc gatttcagat
     1501 gctcgcgaca aatgtattga tagaacgcga ttgcagcttg atggatgatc atgcctttgc
     1561 cgttcatgca aagatggccg gtgaggcggc cgtggcgcaa aaacaccgtc tcctccggat
     1621 gatagatcaa atcatagaag cgcacgtggc gggggatggc gttagccaaa cgaaacgaaa
     1681 cctcatgatt ggtctcgata tcagggcggg cggattgcag ccacgcgccg gcaaaaccgg
     1741 ctgtgtcgag ttcggacaat ggaaaagctg gcgggtgcgc aggggcaaga gcggaatagg
     1801 gctcgaggtt ggtaactctg ccgcccgaca gttggcggat gccgccttgt cctttggtgg
     1861 tgctgttgat gatcaggcca acctcgagca gatcttttcc aatttgagcc tcgctgatcg
     1921 cgaatgcctg gtggccctgc tgtcgcaggt tatccgccag agcaagcgct gtcgggagcg
     1981 tgcggttgct aatcagcagc tttccgccaa ctaaaagatc cgcgatatga aatgcgacgg
     2041 cgcgcgctga tcctccggcg ccgatcagca gcacgttcat gcctgccaga gaggaaataa
     2101 agggctcgga gcgatccgcc gagggttgga gaatgctctg cacgaagcct tcgccgtcgg
     2161 tgttgtagcc gatcagccgt ccggcctggg ttcgcacgat cgtgttgacg gcgtcgattc
     2221 gaagcgcgcc gggatcaatc tcgtcgagaa attccatgat ccgcaattta tgcgggacgg
     2281 ttatgttagc gccccaaaac cgtttgcatt gcctcaatac ggtgagcaga tgcggcaatt
     2341 gatcatcgct ggtatcgaac ggcagataaa ccgcttcgat cccaagctca gcgaaagccg
     2401 cctgccatat gctcgggctt ctcgagtact gcgacggtgc atccccgaca acgccagtga
     2461 tcgtgctttc actcagagtg agttcgccta gcgagttcga tatgcagttt tggattgccg
     2521 aaaggtcagc ggatcgcgtc atagccgtct cgcatgctca ctagtcgcac ggcacgacgg
     2581 gtttggcaac tcaaaaaaaa actgtcaggc gggcttttta atcgagaatt tgccgtcaac
     2641 gaaattcaac agcgtgcgtt gaccgtcgag aagagtctcc atggccacgg ctcttcccca
     2701 gagttgatac agatagcgga gggttttttc ggcgtattcg agttggagat cgcgaccgtc
     2761 atggtcgtgc ttgagcaaca gagcacgatt gttgtggtaa tcggcatctt ctactttgat
     2821 tacgggaatg gtgccggtgc cgacgttctg aattaaagtt tccttgatct ggcgccagtt
     2881 ctcctcgtcc gccacgcggc tcaccacctg ctcattacca cggctcttat actcgaaaag
     2941 gttgagctcg cgaatcaaat cttcggttaa atagcggcgc agaaaagagg cgtcgcgctc
     3001 gacttcacgc acctcgaata atttttcccg cccggtcttt ttgcgcggac caaagttctc
     3061 gaggtcttca gcgctcgggt ggtcccagcg ctgttcgata tcttcccaga ctttcattcc
     3121 cacatggt
//
LOCUS       DASULZ010000497         7178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_973696, whole genome shotgun sequence.
ACCESSION   DASULZ010000497 DASULZ010000000
VERSION     DASULZ010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7178
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973696"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VFY96_10155"
     CDS             complement(<1..639)
                     /locus_tag="VFY96_10155"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5606868.1"
                     /translation="MATAPAVTIEWLGHSTFQITSSKGTRVLTDPHGAFDLPHPTLPQ
                     HIVTTSHQHGPHNSVHMAPGTPVILHGLTPTGEDWQKISTTIRDVSVYAVPAYHDKSQ
                     GMQRGKNAIFVFRVDDICIAHLGDLGHVLSPGQLKMMGKIDILLMPIAGGYFTVTPAE
                     ARDVTKQVNPKIVIPMHFWWEQAVQEFVQGMPKVKMLNTPVLKISKSELPNST"
     gene            complement(856..3114)
                     /locus_tag="VFY96_10160"
     CDS             complement(856..3114)
                     /locus_tag="VFY96_10160"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEX5606869.1"
                     /translation="MSDLYLLGALPWWLVALIAVGAAALLVRQFINLRERLSRGQTTF
                     LTLIRACVYAGLIFFLLGPSLIDKRITKLRRPLTLLIDSSQSMGFPASAKAGAPDKPV
                     NSRLDLVREKLTAGQTPLLQRLSRDYDLRIIRFGTGTEPIAPGNIARLRPQDPGTRLI
                     EVLQSTAKDPAARSGMIVFTDGISNGETQALDGASIGAPVFMVGVGETRGFTDVRIAS
                     FGAPEFAFRGREFKIELTVQAFGMKGKSVPLFFNRGKNLIATRTISIGADPFEQKVTL
                     SFTPKELGTHTFSVSLPPQPGEQIPQNNQREFKVDVGRDKIRVLTLSGSPAWNYRFLR
                     MALKQDPLVELVSFVFLRTPTDTVDVPENQLSLIPFPIDDIFLEELKNFDVVVFDDFS
                     YRAYFNPVYLERVRDFVRDGGGLAMFGGVRSFDNGGYGDSALREVLPVELDGKGNFQA
                     RGDVHAILTPAGKAHPITRLLPDSKANEETWRNMPALTGVNPVRSVRGETLLTAGPDA
                     SQPGVPLLAIGRFAKGRTLTLMSDDAWRWNFIAVGNKETPQNHLKLVRQSIRWLAQEP
                     AFEQVQILPIATTQPGEKVPIKIKVLNDDFTPVRQAAVQLRVFSPEGEPILVPTAPER
                     EDGEYSGEFTPTKEGIYRVEAEASASGKTLGRDKNSFTAAYSYGEMDDGLPRLDLLKK
                     IAESSKGEYISINDWNDRTFDKVAAKLEQIAPSEIVEQRQVRLWSNLWPFGLILTLLS
                     IEWWMRRKWGLI"
     gene            complement(3117..3884)
                     /locus_tag="VFY96_10165"
     CDS             complement(3117..3884)
                     /locus_tag="VFY96_10165"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEX5606870.1"
                     /translation="MNRRQFIRILSFGLTASLLPRVVEAAESDDKRRLQFTFAQIKYR
                     GGDWNPHPLAVTPLLEELMARTSVESANAKHEVTLSDRDLFNYPFLYLAGKYEFEPFT
                     QEEVEILRRFLSYGGFLLIDDALGQQGYGFDKSVRRELQRIFPGNELKRLPSGHAALR
                     SYYLLRRIGGVRIASPNLEGISVGTSTPVIYCQNDLGGAWERDQLGNWTNPCTPGGEP
                     QRKDAFHLGINIILYAMTENYKDDLIHVPFIRRRLTR"
     gene            complement(4001..6094)
                     /locus_tag="VFY96_10170"
     CDS             complement(4001..6094)
                     /locus_tag="VFY96_10170"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HEX5606871.1"
                     /translation="MSVFFLYPVFLFGLAAAALPVLIHLLNRRKLKRIRFPAVRFILL
                     SQKRISRSYRLRHWILLALRTLAIVLLALLLANPIFQTGAGLFAGGGPVALVVLLDNS
                     LSMTWSGDGNGFKQAKDAARLLISALSEGDQAAIIPTNLSADEPFRLKGQKDVLLREL
                     DAIEIADGTANLNIALSKAYELLQQPAGQKEIRLVTDMGLTGWDQFTVAALKQVDPSI
                     PFKLIHIGRKEQPLNGTVKEIRLAGQGVGANLPLQIEATVTNFGEREIKDALAQLTID
                     GQNRGQKLVTVSPRSENTVSFQTRLGQPGAHAAQVILKKDGLAGNSSTNFTLDAQDKL
                     KVLVVDGDPQTSLIQSETFFLSRALNPSGESDGSVFLPTVILADGLNNASLESYQVVI
                     LCNVGSLPDSFTAKLPSFLQAGGGLLIFSGDKFQPEIYNQRLSRDLPGQVGEKKLAPE
                     ASGEKIGKFDADHPALQFLSDSILQESVKSARVWGYFRTAGTRSPLISLANGDPLLVE
                     QKIGAGKAIFMTTAADRDWTDLPLKTAYLPLIQSLTQYLAGGKRGGLDAGIAVGSIKE
                     LALPPSYVGKNLRLTKPDKQTRDVQINAAKDRAVAAIEYNDRAGFYRLSVPAGGEKDR
                     AVPQVYAVNPPFLESRLDEISASELQAKLKPIRPEVVPVEALKEGGKRTDLALPLVAL
                     LMITLLLEGWLGGRF"
     gene            complement(6282..>7178)
                     /locus_tag="VFY96_10175"
     CDS             complement(6282..>7178)
                     /locus_tag="VFY96_10175"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5606872.1"
                     /translation="NPMATHDKNYFDPKVLAGISNLALRARWVVEGLMSGVHRSRAKG
                     FSVEFEEHREYSPGDEIRRIDWKALGKFDRYYIKEYEDETNLRAYLVLDASSSMNYAS
                     DGISKFDYGCTLTASLAYLILKQQDSAGVVTFSDRVEAFIPPRAKRDYLTQILHALEN
                     RGPGGETHIGKILEEIAGQVRRRGLIVLISDLLDDGDAILKGLRHFRFKGNDVIVFHL
                     LDQAELDLPFEGNILFEDLEAANLQVIADPRAIRSTYQQVVQEFIGNVRKQCHDSAID
                     YQIISTATPLDRALASYLSWRG"
BASE COUNT         1563 a         1983 c         2038 g         1594 t
ORIGIN      
        1 ggtggagttc ggcaattcgg atttggatat tttcagtacg ggcgtattca acatcttcac
       61 tttaggcatg ccttgaacga actcttgcac ggcttgttcc caccagaaat gcatagggat
      121 cacgattttc gggttgacct gtttggtgac gtcgcgggct tcggcaggcg tgacggtgaa
      181 gtagccgccg gcgatgggca tgagcagaat gtcaattttg cccatcattt tcagttgccc
      241 agggctcaaa acatggccca agtcgccgag gtgcgcgatg caaatgtcat cgactcggaa
      301 gacaaaaatg gcgtttttgc cgcgttgcat gccctggctt ttgtcgtgat aagcgggcac
      361 ggcgtaaacg gaaacatctc ggatggtggt tgagattttt tgccagtctt cgcccgtcgg
      421 cgtgagtcca tgtaggatca ccggcgtgcc gggcgccata tgcacgctat tatgggggcc
      481 gtgctggtga ctggtggtga cgatgtgctg cggcaacgtc ggatggggca agtcgaaagc
      541 gccgtgtgga tccgtcaaaa cgcgcgtccc cttcgatgaa gtaatttgaa acgtcgaatg
      601 gcccagccat tcaatagtca cggcgggagc ggttgccacc gcaactctca tgaaagcgtc
      661 ttgaaccact tcgagctcat ggcaaaaggc caaagcgcgg gccggtatca ggatagacaa
      721 caaaatcacg agcaacaaat ctgttcttag tttcgggcac actttgagct acgcgttctg
      781 gcgttgaaaa gcctggtgtc tagggtctcg cgttgaggta ctctaaacgt tagacccgag
      841 gcgctagaca atgaattaga tgagtcccca tttccgtctc atccaccact cgatgctgag
      901 cagggtaaga atgaggccga atggccatag attgctccaa agacggacct gccgttgctc
      961 gacgatttcc gagggcgcga tttgttcgag cttcgcagcg actttgtcga aggtacggtc
     1021 attccaatca ttgatcgaaa tgtattcgcc cttgctcgat tcggcgatct ttttcagtaa
     1081 atcgagacga ggcaagccgt catccatttc gccgtaggag tacgccgcag taaaactgtt
     1141 cttgtctcta cccaaggttt tgccgcttgc actcgcctcg gcttcgaccc gataaattcc
     1201 ttctttggtc ggcgtaaact cgccactgta ttcgccgtct tctcgttccg gcgcggttgg
     1261 caccagaata ggctcgcctt ccgggctgaa cacacggagc tgaaccgcgg cctggcgcac
     1321 cggcgtgaag tcgtcattca atactttgat tttgatcggg accttttctc ccggctgggt
     1381 ggtcgcgatc ggcaaaattt gcacctgctc gaaggcgggt tcctgggcga gccaccggat
     1441 cgactgacga acaagtttca aatggttttg cggcgtttct ttgttgccga ccgcgatgaa
     1501 gttccagcgc caggcgtcat cactcatcag cgtcagagtt ctacctttgg caaagcgtcc
     1561 gatcgcaagc aatggaacgc cgggttggga cgcatcgggc cctgccgtga gcagtgtctc
     1621 gccgcggacg ctgcgcaccg ggttaacacc ggtgagcgcg ggcatgttgc gccaagtctc
     1681 ctcgttggct ttgctgtcgg gcagcaagcg cgtgatcggg tgcgctttgc cggccggcgt
     1741 gagaatcgca tggacgtccc cgcgtgcttg aaaattgccc ttgccatcga gctccaccgg
     1801 cagcacttcg cgcaatgcgc tgtcgccata gccgccgttg tcgaaggagc gcacgccgcc
     1861 aaacatcgcg agcccgcccc cgtcgcggac aaaatcgcgc acccgctcca ggtaaacagg
     1921 attaaagtag gcgcgatagg aaaaatcatc gaagacaacc acgtcgaagt tcttgagctc
     1981 ttccaaaaaa atatcgtcga tcgggaatgg aatcaggctc aactgatttt cgggaacgtc
     2041 caccgtatcg gtcggtgtgc gcaaaaacac aaatgagacc agttccacca acggatcttg
     2101 tttcagcgcc atgcgcaaaa accggtaatt ccacgccggc gaacctgata gagtcaagac
     2161 gcggatcttg tcgcgtccga catcgacttt gaattcccgc tgattgtttt gcgggatttg
     2221 ttcgccgggc tgtggcggca ggctaaccga aaacgtatgg gtgccgagct ctttgggggt
     2281 gaagctcaat gttaccttct gctcgaaggg gtccgcgcca atgctaatgg tgcgcgtggc
     2341 gatgagattt tttccgcggt tgaaaaacag cggcacggat ttgcccttca tgccgaaagc
     2401 ttgaaccgtc agctcgatct tgaactcacg gccgcgaaat gcgaattccg gcgcgccgaa
     2461 actggcgata cgaacatcgg tgaatccccg ggtttcgcca acgcccacca tgaacaccgg
     2521 ggcgccgatg cttgcgccgt ccagagcttg ggtctcaccg ttggaaatcc catcagtgaa
     2581 tacgatcatt ccggagcgcg ccgccgggtc cttggccgtg ctttgcagca cttcgatcaa
     2641 gcgggtgccg ggatcctgag gccgtagccg ggcgatattt cccggcgcga tcggctcggt
     2701 tccggtaccg aaacgaatga tgcgtaaatc gtagtcgcgg ctgagtcgtt gcagaagcgg
     2761 agtttgacct gcggtgagtt tttcccgcac cagatccagc ctactgttca ccggcttgtc
     2821 cggcgcacct gctttggcgc tggcgggaaa acccatgctt tgggaactat cgatcagcaa
     2881 cgtgagcgga cggcgaagct tggtaatgcg cttgtcgatt aggctcggac ccaaaaggaa
     2941 aaaaatcaaa ccagcataaa cacaagctct aatcagcgtg agaaaggttg tttgtccgcg
     3001 cgagaggcgc tcgcgtaaat taataaactg acgcaccaaa agtgcggctg caccgaccgc
     3061 gataagcgcg acgagccacc agggcaaggc gccaagaaga tagagatcgc tcatgtttac
     3121 ctggtcagac gccggcggat gaacggaacg tggatcaaat cgtctttata gttttcggtc
     3181 atcgcgtaca ggataatatt gataccgagg tgaaacgcat ccttgcgctg cggttcgccg
     3241 cccggagtac atgggtttgt ccaattgccc aactggtcgc gctcccaggc gccgccaagg
     3301 tcgttttggc aataaatcac cggtgttgac gtgccgacgg aaatcccttc gagattcggg
     3361 ctggcgatcc gcacgccgcc gatccggcgc aaaagataat agcttctgag cgccgcatgc
     3421 cccgacggca gacgcttgag ttcattgccg ggaaagattc gttgcagctc gcggcgtact
     3481 gacttgtcga agccgtagcc ttgctgaccg agcgcatcat cgatgagcag aaaaccacca
     3541 taactcagaa agcgccgcag tatttcgacc tcctcctgag taaagggttc gaattcgtat
     3601 ttgccggcta aataaagaaa cgggtaattg aacaaatcgc gatccgataa ggtcacttcg
     3661 tgctttgcgt tggccgattc gacgctcgtt cgcgccatca gctcttccag cagcggcgtc
     3721 acggccaaag ggtgcgggtt ccaatcgccg ccccgatatt tgatttgcgc gaacgtaaac
     3781 tgcaaccggc gcttatcgtc ggattcggcg gcttctacca ctctgggcag tagcgaggcg
     3841 gtgagaccga atgaaaggat tcgaatgaat tgacggcgat tcatctactc atttctctta
     3901 ccacgaagca cacgaagctc acgcagagaa ggttctaaac acttcgtgtc cttcgcgccc
     3961 ttcgtggtga accccgcttt ctttctccgt gctccgccct ctaaaaccgc cccccgagcc
     4021 agccttccag cagcaatgtg atcatcaaga gcgccaccag cggaagagcg agatcggtgc
     4081 gcttgcctcc ctccttaagc gcctcgaccg ggacgacctc ggggcgtatg ggtttgagct
     4141 tcgcttgcaa ttcgctcgcg ctgatttcgt cgagacgaga ttcgagaaac ggcggattga
     4201 ccgcataaac ctgcggcacc gcgcggtcct tctcgccgcc cgccggtacg gacagccgat
     4261 aaaaaccggc gcgatcgtta tattcgatcg cggcgacggc gcgatccttc gccgcgttga
     4321 tctggacatc acgggtttgc ttgtccggct tcgtgaggcg gagatttttt ccaacgtagc
     4381 tcggcggcag cgccagctct ttgatgctcc cgacggcaat gccggcgtcc aagccgccgc
     4441 gttttccccc agcgaggtac tgggtcaaag attggatcaa cggcagataa gcggtcttca
     4501 gcggcagatc cgtccaatcc cggtcggccg ccgtggtcat aaaaattgct tttcccgcac
     4561 cgatcttttg ttcgactaat agcggatcac cgttagccag tgaaatcagc ggtgatcgtg
     4621 tgcccgcagt tcggaaataa ccccagaccc gtgccgattt gaccgattcc tgcagaatcg
     4681 aatcggataa aaactgcagt gccggatggt cggcatcgaa tttgccgatc ttttcgccac
     4741 tggcttccgg agcgagtttt ttttcgccca cttggccggg caaatcgcgt gacaatcttt
     4801 gattgtaaat ttccggctga aacttatcgc cgctaaaaat caacaggccg ccgcccgctt
     4861 gcaaaaaact cggtagcttc gcagtgaagc tatcggggag tgagccgaca ttgcacagaa
     4921 tcacaacttg atagctttcc agcgaggcgt tgttcaagcc gtcggccaag atcacggtag
     4981 gaagaaaaac cgaaccatcg ctctctcctg agggattgag ggcccggctc agaaaaaaag
     5041 tttcgctttg aatcaaggat gtctgtggat caccgtcgac caccagaacc ttgagcttgt
     5101 cttgcgcatc gagggtgaaa ttggtgctgg agttgccggc caagccgtct tttttgagga
     5161 tgacctgtgc cgcatgtgcc ccaggttggc cgagtcgggt ttgaaagctg acggtatttt
     5221 ccgatctcgg cgataccgta acgagcttct ggccgcgatt ttggccgtcg atcgtgagtt
     5281 gcgccagcgc atccttgatc tctcgttcgc caaagtttgt cacggtcgct tcgatctgca
     5341 agggcagatt ggcgccgaca ccttgacccg ccaagcggat ttctttcacc gtaccgttga
     5401 gcggctgttc ttttctgccg atatgaatca acttgaacgg gatggacgga tcgacttgtt
     5461 tcaaggcggc cacggtaaat tgatcccagc cggtaagtcc catgtcggtg accaaacgaa
     5521 tttctttttg cccggccggt tgctgcagca actcatacgc tttgcttaag gcaatgttaa
     5581 gattcgctgt gccatccgca atttcgatgg cgtccagttc cctcaagagg acgtcctttt
     5641 gacccttcag tcgaaacggt tcatcagctg ataaattggt cggaatgatt gcggcttgat
     5701 cgccctcgct caaggccgag atcaacaggc gcgctgcgtc tttggcttgt ttgaaaccat
     5761 tgccgtcacc gctccaggtc atgctgagtg aattgtcgag cagcacaacg agcgcaaccg
     5821 gcccgccgcc ggcgaaaagt ccggcgcctg tctggaagat cggattggcc agaagaagcg
     5881 cgagaagcac gatggccaaa gtcctgaggg ccagtaaaat ccaatgccgc aaacggtagc
     5941 tgcgcgagat gcgtttttgc gaaagcaaga taaaacgtac ggcgggaaac cggatgcgct
     6001 tcaattttcg ccggttgagg agatgaatca ggaccggcaa ggcggccgcc gccaggccga
     6061 agagaaaaac cggataaaga aaaaaaaccg acatcagcgc tcgtcgcttg agaatgaaat
     6121 cgcgtgaacg agattttgct ttttcgtcat ggcaccgctg aaagctcgcg ccaaacccga
     6181 tcggcgccaa gcgtccggcg gacgcatgcc atgcgcccgt gcgactgtag gtcgatgccg
     6241 aatgacgatg ctatctttcc gcgcggtgtg cctgaaaaac actagcctct ccaactgaga
     6301 tagctggcga gcgcgcgatc gagaggggtg gcagtggaga tgatttggta atcgatggcg
     6361 ctatcgtgac actgcttgcg aacattcccg atgaactcct gcacgacttg ctgataagtc
     6421 gagcggattg cgcgcggatc ggcgatgact tgcagattcg ccgcttccaa atcctcgaac
     6481 aggatattgc cctcgaaggg caagtcgagc tcggcctgat cgagtaagtg aaatacgatg
     6541 acgtcattgc ccttgaaacg aaaatggcgc agtcccttga ggatggcgtc gccatcgtcg
     6601 agcaggtcgg aaattagaac gatcagcccg cgccgcctga cctggccggc gatctcctcg
     6661 agaattttac cgatatgggt ttcgccaccg ggaccgcggt tttccaacgc atgcagaatc
     6721 tgcgtcagat aatcgcgctt cgcccgaggc ggaatgaatg cttcgacgcg gtccgaaaac
     6781 gtgaccactc ctgcggagtc ttgctgcttg agaatcaagt aggccaggga ggcggtcaag
     6841 gtgcagccgt aatcgaactt gctaatgcca tcggacgcgt agttcataga ggagctggcg
     6901 tcgagcacca gataggcgcg cagattggtt tcatcctcgt actctttgat atagtagcgg
     6961 tcgaattttc cgagcgcttt ccaatcgatg cggcggattt cgtcccccgg cgagtactcg
     7021 cggtgctctt cgaactcaac gctgaagccc ttggcgcggc tccggtgcac acccgacatc
     7081 aacccttcga cgacccagcg ggcgcgcagc gcgaggttcg agattcccgc cagcactttg
     7141 ggatcgaagt agttcttatc gtgggtcgcc atagggtt
//
LOCUS       DASULZ010000498         2621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1480791, whole genome shotgun sequence.
ACCESSION   DASULZ010000498 DASULZ010000000
VERSION     DASULZ010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480791"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VFY96_10180"
     CDS             <1..334
                     /locus_tag="VFY96_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2255 family protein"
                     /protein_id="HEX5606873.1"
                     /translation="KITSLRHDGTLTKPVTIWVVRDGDDIYVRSVNGRNAAWFRGTQV
                     RREGRIETGGLHRDVTVVDVDPEINDQLDAAYRTKYRHYAASIVNSIMTSKARSTTMK
                     VVPRPTSF"
     gene            368..1405
                     /locus_tag="VFY96_10185"
     CDS             368..1405
                     /locus_tag="VFY96_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX5606874.1"
                     /translation="MRATVMYSAGDVRIENIPDARLIEPADAVLRVTRACICGSDLWP
                     YKGLEEFPETGRRMGHEAIGVVDAVGADVRTIKRGDLVIMPFAFSDGSCIFCNQRLQT
                     SCIHGGFFGIHGNDGAQAEALRVPQADGTLFALPVRKDDALMPSLLTLSDVMGTGHHA
                     AVCAKVGPGKKVAVIGDGAVGLCGVIAAKRLGAEQIMILGRHADRIALAREFGATDIV
                     SERGDEAIERVRKLTGGFGVHSVLECVGLEQSMLTAISIARPGGAVGRVGVPQAETMP
                     ASLPAFYNNVTVSGGPAPVRAYIDELLPDILDGRIEPGRVFDRVVDLDGVPDGYRAMN
                     ERESIKVMVEP"
     gene            1551..>2621
                     /locus_tag="VFY96_10190"
     CDS             1551..>2621
                     /locus_tag="VFY96_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX5606875.1"
                     /translation="MSELEHLLVLVLAAVLLAALARRIGAPYPVFLALGGALLALLPT
                     VPLFTVPPELALALFIAPVLLDAAYDASPRDLKDNWAPLTSLVVFAVGLTTAAVAVVT
                     RTLVPEMPWAPAIALGAVVAPPDAAAATAVLRPLRPPHRILTILEGESLLNDASALLI
                     FRLAVGAVAASSFSIGSVAPTFMLAVAGSLIAGPALGWLVVRVLDRVQHMPTAIILQF
                     ATTFGVWILAESIGLSGVLTMVFYAMTLARTSPERTPARIRIPTYAVWETIVFVLNIL
                     AFIFIGLQIRPIVESLPATDRGRYLAVAGAVLLTVIVVRLVWHMSFNTVMRWRQRRSG
                     FHPPRPMLRPTLGSGLIISWAGM"
BASE COUNT          480 a          797 c          802 g          542 t
ORIGIN      
        1 gaagatcacg tcactgcgac acgacggtac gctcacgaaa ccggtgacga tttgggtcgt
       61 gcgcgatggc gatgacatct acgtgcgatc cgtcaatggt cgtaacgccg cgtggtttcg
      121 tggcacgcag gtgcgccgtg aaggacgtat cgagaccggc ggactccaca gagatgtcac
      181 ggtcgtagac gttgatcccg agatcaacga ccagctcgat gccgcgtacc ggacaaagta
      241 ccggcactat gcagctagca tcgtcaacag cattatgact tccaaggcac gttccacgac
      301 aatgaaagta gtgccacgcc caacaagctt ttaacgtcaa caattatgga aagcagaggt
      361 aacctttatg cgcgcaactg ttatgtacag cgccggggac gttcggatcg agaatattcc
      421 cgacgctcgt ctcattgagc cggccgacgc tgttctcagg gtcacacgcg cctgtatctg
      481 tggcagcgat ctgtggccgt ataagggact ggaagaattc ccggagaccg gccgccgcat
      541 gggacatgag gccatcggcg ttgtcgatgc ggtcggcgcc gatgtccgca caatcaaacg
      601 cggcgatctc gtgattatgc cgttcgcctt ttcggacggg agctgcatct tctgcaacca
      661 aagactacag acgtcctgta ttcacggagg atttttcggc atccatggaa acgatggagc
      721 gcaggccgaa gcgttgcgcg tcccgcaggc ggatggaaca ctgttcgcac tgccggtccg
      781 caaagacgac gcgctgatgc catcgctgct caccctttca gacgtaatgg gaaccggcca
      841 ccatgcggcg gtttgcgcca aggttggtcc tgggaaaaaa gtcgccgtca tcggtgacgg
      901 cgcggtcggc ttgtgcggcg ttatcgccgc gaagcgtctc ggcgccgaac agattatgat
      961 cctgggccgg catgcggacc ggatcgcgct cgctagagaa ttcggcgcca ccgacatcgt
     1021 gagcgagcgc ggcgacgagg cgatcgagcg cgtgcgcaaa ctcaccggcg gtttcggcgt
     1081 ccactccgtg ctggagtgcg tcggactcga gcagtcgatg ctcaccgcga tcagcatcgc
     1141 gcgcccgggt ggcgcagtcg gtcgcgtcgg ggttccacag gctgaaacca tgcccgcttc
     1201 gcttccggcg ttttacaaca acgtgactgt gagtggcgga cccgcgccag tgcgcgctta
     1261 catcgatgag cttttgccgg acattctcga tggcagaatc gagcccggcc gggtcttcga
     1321 tcgggtggtc gacctcgacg gcgtgcccga tggctatcgg gcgatgaacg agcgcgagtc
     1381 gatcaaggtc atggtcgagc cctgatgaaa gtcgcagcgc agaccagctc catcgcagcg
     1441 tagcgcgctg aagccgtaag gaaacaaatt cagaaacttc accgcggaca caccgaggac
     1501 atagagttcg gcgggagaaa gatcggtgca cgtccaatcg cgcctgacat atgagcgagc
     1561 tcgaacacct tcttgtgctg gtactcgcag ccgtgctgct ggcggcgttg gcacgccgca
     1621 tcggcgcgcc ttatccggta tttctcgcac tcggcggcgc gctcctcgca cttctgccaa
     1681 ctgtaccctt gttcaccgta cctcctgaac tcgcgctggc gcttttcatc gcgcccgttc
     1741 tgcttgatgc ggcgtacgac gcatcgccgc gagatctcaa ggacaactgg gcaccgctta
     1801 caagcttggt tgtgtttgcg gtgggattga caaccgccgc tgtggccgtc gtcactcgca
     1861 cgctggtgcc cgaaatgccc tgggcacccg cgattgcgct cggtgcggtg gtcgctccac
     1921 cggatgccgc tgccgccact gctgtgctcc gcccccttcg accgcctcat cgcattctga
     1981 ccatcctcga aggcgagagc ctgctgaatg acgcgagcgc gctgctgatt tttcggctcg
     2041 cagttggagc cgtcgcagcc agtagttttt cgattggcag tgtcgcgcca actttcatgc
     2101 tcgcggtggc cggcagccta atcgcaggtc ccgcactggg gtggctcgtc gtgcgcgtgc
     2161 ttgaccgcgt gcagcatatg ccaactgcca tcattcttca gttcgccacc accttcggcg
     2221 tctggattct ggccgagagc attggcctct cgggtgtgct gacgatggtg ttctacgcaa
     2281 tgaccttggc gcggacctcg cctgagcgta ctccggcgcg gatacgcata ccgacatacg
     2341 cggtatggga gacgattgtt ttcgtgctga acatcctggc gttcatcttc attggcctgc
     2401 agattcgccc gattgtcgag agcctgccgg caaccgatcg cggccgctat ctcgctgtag
     2461 caggcgccgt cctcctgacc gtgatcgtcg tgcgcctcgt ctggcacatg tccttcaata
     2521 cagtgatgag gtggcgacag cgccggagtg gttttcatcc gccgcgtcca atgctgcgcc
     2581 ctacgctcgg gagtggtttg atcatctcat gggccgggat g
//
LOCUS       DASULZ010000499         6039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_973727, whole genome shotgun sequence.
ACCESSION   DASULZ010000499 DASULZ010000000
VERSION     DASULZ010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6039
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973727"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /locus_tag="VFY96_10195"
     CDS             complement(<1..1379)
                     /locus_tag="VFY96_10195"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5606876.1"
                     /translation="MYDLLVKGGRIYDGSGMPSSYADLGISGGKIVEIGRLNGGAKRM
                     LNVDGLAVAPGFIDPHTHLDAQLFWDPLGTSSCFHGVTSVVVGNCGLSLAPAKPEDRD
                     AVIKSFVRVEAISRRVLEEGIQWKWESTSEYLDALGARLGINAAALVGHIAVRHSVMG
                     EDAVERRATAEEIAKMKELVRQGMEAGAVGFSTNQNPRHIREDKKPVASRLASDEELG
                     SLLDVLAEMNSGVVQLSGGGADARGRIAYAAQMARQTGRPVLWQSINHSWSRPNHWQE
                     MLANTQRVFKEQGLPIYAMTQAKPFQNRYTLLDAQCFDEFPTWKSAMFSPVEARKQMF
                     ADSAIRKKLRAEAIEDQSPSVFPRRWDVIFVDHVKLPKNKIFEHKSVQEIASAADKDG
                     LDWFLDISLEEDLQTRFVHTNTQGDPQAVCEILKHPAVMIGQSDAGAHMGYDARFGYS
                     TAFLGCWVR"
     gene            complement(1570..2064)
                     /locus_tag="VFY96_10200"
     CDS             complement(1570..2064)
                     /locus_tag="VFY96_10200"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5606877.1"
                     /translation="MKKISRFCRPGARVSTVCIVPVVCYSRVMSRIPGLKGMRHIALK
                     VKDVARSKRFYQDVLGMDVVWEPDPQNVYLSSGCDNIAIHGVSEAFANTAEERQLDHL
                     GFVVEDVERVRALENEFIAKGVPIVHRFKVHRDGSASFYCADPDGIIIQLLYEPQLSR
                     QTIE"
     gene            2063..3109
                     /gene="amrS"
                     /locus_tag="VFY96_10205"
     CDS             2063..3109
                     /gene="amrS"
                     /locus_tag="VFY96_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEX5606878.1"
                     /translation="MKEALWWQTEADGKILCTLCPRFCRVGDGQAGFCYIRRNVGGKL
                     YSLGYGTSTGFAIDPIEKKPLNHFLPGSEILSFGTAGCNLGCRFCQNWSISKAKLDEA
                     ESYHVAPEQVVELALRKQVPSIAFTYNDPVIWGEFVIDISRHAREHGINSVMVTAGYI
                     TPEARPEVFRYIDAANVDLKAFTERFYHKLTFSHLTPVLETLVWLRRETDIWFEITNL
                     MIPGENDEPDETRRLCDWVAENLGDSVPLHFTAFHPDFKLTDKPRTPASTLKQARAIA
                     QASGIKYCYVGNIHDDEGQTTRCPACQSVLIRRSWHEIIDNKLSNGCCACGQVIPGRF
                     ATRTSSTPRQSILG"
     gene            3335..5743
                     /gene="ptsP"
                     /locus_tag="VFY96_10210"
     CDS             3335..5743
                     /gene="ptsP"
                     /locus_tag="VFY96_10210"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX5606879.1"
                     /translation="MAKLKTKRNRRIAPPPHLDILEDISTLISHSHDLQETLDRIVAT
                     VADRMQTEVCSIYILDPKTNRLTLRATMGLDPESVGKVSMGISEGLTGMVIERMKPVM
                     VADTLAHPRYKYFPETHEEHFHSFLGVPLIEGRLPLGVLVVQTSRRRHFSRDEIRLLT
                     TISAQAASIIVQGRLAESLKSKEEERKEFEKRMNEAMRRLRSYEGTRREKQAKSKQHW
                     RGRLNGLAASPGFGRGRAFVLEPRMDLAAISKKKARNPEREMERFRAAVERGIEQVNV
                     VKNRMRQFIPKEENAIFDVYRLILEDPAIIQQIEQQIFKEGYTAEYSIRVVFERYLQS
                     IANIKDSYLRERTTDVKDAAQRLLDNLSGVSGQQYDLPDDAVLVAEDLSPADLSMLEG
                     DKFKGIALATGGVTSHASILAKSFEIPSVVAIEGLMEAVHQGDMLIVDGNAGNVHVNP
                     NVEVIREYNRLERDYAALNRELGALKNLPAETTDGRRVSLYANIGLLSDVAFAQLHGA
                     QGVGLYRTEIPFLSHRDFPSEDEQYSLYSRVVEGMAGKPVTIRTLDIGADKYPTYMRS
                     PTVEPNPFLGWRSIRISLEVEEIFKTQLRAVLRVGELGRVRLLIPMVTSLEEVLKVKE
                     ILAEAKAELDHEGTPYDRQMELGIMVEVPAAVQLADRFLREVDFLSIGTNDLIQYILA
                     VDRSNRKVASLYEPLHPAVLKSLSTTIDAGKRQGKRVGMCGEMAGDPLCAILLLGMGL
                     EEFSTGSLYIPVIKKAIRSISYQSAKASAEIVLAMDAVGEIKRYLFEQMRQLGMVELL
                     EMYR"
     gene            5846..>6039
                     /locus_tag="VFY96_10215"
     CDS             5846..>6039
                     /locus_tag="VFY96_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606880.1"
                     /translation="MTKISTFDDWIDYFREWQRDIGYDPTLLGDYKFETKLGELHSPE
                     IEFGDYKGQQKWQRVTQIPN"
BASE COUNT         1388 a         1733 c         1607 g         1311 t
ORIGIN      
        1 tcgcgcaccc agcaaccgag aaacgccgtc gaatacccga agcgagcatc atagcccata
       61 tgagcgccgg catcggactg accgatcatg acagccgggt gtttgagaat ctcacaaacc
      121 gcctgcgggt cgccttgcgt attggtatgg acaaagcggg tttggaggtc ttcttcgagg
      181 gagatatcca ggaaccaatc cagaccatct ttatctgcag cgctagcaat ttcctgcaca
      241 ctcttgtgct cgaaaatttt gttcttcggc agcttcacat gatcgacaaa aatcacgtcc
      301 cagcggcgcg gaaaaaccga aggagattgg tcttcgatcg cctcggcgcg aagtttcttc
      361 cggattgcgg aatccgcgaa catctgcttg cgcgcctcca cgggactgaa catcgccgat
      421 ttccaggtcg gaaattcgtc gaagcactgc gcatctaaaa gcgtataccg attttgaaat
      481 ggtttggcct gcgtcatcgc ataaatcggc agcccctgtt ctttaaaaac gcgttgggtg
      541 tttgcgagca tctcttgcca gtggttcggt cgactccagc tgtgattgat gctttgccac
      601 agcaccggcc gtccggtttg gcgcgccatc tgcgcagcgt acgcgatacg gccgcgcgcg
      661 tcggcaccgc cgccactcag ctgaacgact ccagaattca tttcagccaa gacatccaac
      721 aaagagccca actcttcgtc cgacgccaga cggctcgcca ccggtttttt atcttcgcga
      781 atatgacgcg ggttttgatt ggtggagaat cccacggcgc ccgcctccat tccctggcgc
      841 acgagctctt tcattttggc gatctcttcg gccgtcgccc gccgttctac ggcatcttcc
      901 cccatcacac tgtgccgcac ggcaatatgg ccgaccaacg ccgcggcatt gattcccagc
      961 cgcgcaccga gtgcgtccag gtattcactt gtggattccc atttccattg aattccctcc
     1021 tcgagcacgc ggcggctgat cgcctcaacg cggacaaaac tcttgatcac cgcgtcgcgg
     1081 tcttcgggtt tcgccggcgc caagctcaag ccgcaattgc cgacgaccac agaggttaca
     1141 ccatggaaac acgaagaggt gccgagcggg tcccaaaaca actgcgcatc gagatgcgta
     1201 tgcggatcga taaatcccgg cgccacggcc aatccgtcga cattcagcat gcgttttgcg
     1261 ccgccattca gccgtccgat ctcgacaatt ttgccgccgc ttataccgag gtcggcataa
     1321 gagcttggca tccccgaacc gtcatagatt cttccgccct taactaaaag atcgtacata
     1381 ggttgcgctc ctgttcacga acaggtttca gagccggaaa aagaagttag tcgatggccg
     1441 cgacgatcgt caagagatgt aggaaagcgc ggacgatgcg gagacccaaa tgggtcagac
     1501 gaacacccag ccgtcgggca ggtgaatcgc cccggcacgg acatcgaggc cgtcggcgcg
     1561 gaccagggat cactcgatcg tttgccggct taactgcggt tcatagagca attgaatgat
     1621 gatgccgtcg gggtcggcac agtagaaaga ggcactgcca tcgcggtgca ccttgaatcg
     1681 atgcacaatc ggcacgcctt tggcgataaa ctcattctcc agcgccctca cacgctcgac
     1741 gtcctccaca acaaacccaa ggtgatcgag ttgtctctcc tccgctgtat tcgcaaaagc
     1801 ctctgagacg ccgtggatcg caatgttatc gcagcctgag gataaataga cgttttgcgg
     1861 atcaggctcc cagacgacgt ccatgccaag cacgtcctga tagaatcgct tcgatcgcgc
     1921 cacgtccttc actttgagcg caatatggcg catgcccttg agacccggaa ttcggctcat
     1981 gacccgacta tagcacacga ccggcacaat gcagacggtg gagactcttg ccccagggcg
     2041 gcaaaagcgg gatatttttt tcatgaaaga agccctttgg tggcaaaccg aagccgacgg
     2101 aaagatactc tgtacattgt gcccgcgctt ttgccgggtc ggcgatggcc aagccgggtt
     2161 ttgctacata cgcagaaacg tcggcggcaa gctttattca ctcggctacg gcacgtccac
     2221 aggttttgcg atcgatccca tcgaaaaaaa gcctctcaac cacttcctgc ctgggagtga
     2281 gattcttagc tttggcacgg ccgggtgtaa tttgggctgt cgcttctgcc aaaactggag
     2341 catcagcaag gccaagcttg acgaagccga aagttatcat gttgcaccgg agcaggtggt
     2401 tgagctcgct ttgcgtaaac aagtgccgag catcgctttc acttacaacg atccggtgat
     2461 ctggggtgaa ttcgtcatcg acatctcgcg tcatgcccgt gagcacggaa tcaacagcgt
     2521 gatggtgacc gccggttaca tcacacctga ggcccgcccg gaggtgttcc gctacatcga
     2581 tgccgccaac gtcgatctga aggcttttac ggagaggttt tatcacaagc ttaccttttc
     2641 ccatctcact cccgtgctcg aaaccttggt ttggctcaga cgcgaaacag acatctggtt
     2701 cgagattact aatttgatga taccgggcga gaacgacgag ccggacgaaa cccgacggct
     2761 gtgtgattgg gtcgcggaaa acctcggcga ttcggtgccg ctacatttca cggcgtttca
     2821 tcccgatttc aaactgaccg ataaaccgcg caccccagca tcgacgctca aacaggcgcg
     2881 cgcaatcgcg caagcgagcg gcatcaaata ctgctacgtc ggcaacattc atgacgacga
     2941 gggacagact acccgttgcc ctgcttgtca aagcgttttg atccgccgtt cctggcacga
     3001 gatcatcgac aacaagttgt ctaacggctg ctgcgcttgc ggccaagtta ttcccggccg
     3061 ttttgccacg cggacgagct ccactccccg ccagtcaatc ctcggttaac ctttactgct
     3121 cgaacgagcg gttcctaagc gttcagctca gctcaagacc acggcgaaag atttttatag
     3181 ttgagctcga gctttgccga aggctttagc tcgactgcgt aagctatttt tatctcgaag
     3241 acgtgacata gcttccctca cgtgagccac tgtcacgcat tcctcactgg tgaaacacta
     3301 gaatttgtgt ttataacaga ctcttggttt ttccatggcc aaactgaaga ccaaacgcaa
     3361 tcggcgcatt gctccgccgc ctcatctcga tattctcgag gatatcagca cgctgatcag
     3421 ccactcgcac gatctgcaag aaaccctcga tcgaatcgtc gccactgtcg ccgaccggat
     3481 gcagaccgaa gtctgttcca tctacattct cgacccgaaa acaaaccggt tgactctgcg
     3541 ggccacaatg ggattggacc ccgaatcggt cggcaaggtt tccatgggga tcagcgaagg
     3601 tctcaccgga atggtaatcg agagaatgaa gcccgtcatg gtcgccgata ccctcgctca
     3661 cccacgttat aagtattttc ctgaaaccca cgaggagcat tttcactcat ttctcggcgt
     3721 gccgctgatc gagggcaggc ttcccttagg cgtcctggtg gtccagacat ctcgccgtcg
     3781 acacttttcc cgcgatgaga ttcgcttgct cacgacgatc tcggctcaag cagccagcat
     3841 cattgtgcag ggccggctcg ctgaatctct caagagcaag gaagaggagc gcaaggaatt
     3901 cgagaaacga atgaacgagg cgatgcgcag gctccgctcc tatgagggca ctcggcgcga
     3961 aaaacaagca aaatcgaagc agcattggcg cgggcgcttg aacgggcttg ctgcgtctcc
     4021 cggcttcggt cgcgggcgag ctttcgtctt ggagccgcgc atggacctcg ctgctatcag
     4081 caaaaagaaa gcgcgcaatc cggagcgcga aatggaaaga tttcgcgccg ccgtggagcg
     4141 cggcatcgag caagtaaatg tggtcaagaa ccgcatgaga cagtttatcc ctaaagagga
     4201 aaacgccatt ttcgacgtct accgtctgat cttggaagac ccggcgatca tccagcagat
     4261 cgagcagcaa atttttaaag aaggctacac cgctgaatat tcgattcgcg tcgtgttcga
     4321 gcgctacttg cagtccattg ccaatatcaa agatagctat ctccgtgaac gcactaccga
     4381 cgtcaaagac gccgcgcaac gactgctcga taatttatcg ggcgtgtcgg gccagcaata
     4441 cgatttaccc gacgacgcgg ttctcgtggc ggaagacctc tcacccgccg atctgagcat
     4501 gctcgaaggc gataagttca agggtatcgc actggccacc ggcggcgtga cttcgcacgc
     4561 atcgattctg gcgaagtcct tcgaaattcc cagcgtcgtc gcgatcgaag ggttgatgga
     4621 agccgttcat cagggcgata tgctgatcgt cgacggcaat gccggaaacg tgcacgtgaa
     4681 tcccaatgta gaggtcatcc gggagtacaa tcgcctggag cgtgattatg ccgcgctgaa
     4741 tcgcgagctg ggcgcgctca agaatcttcc ggcggaaacc accgacgggc gcagggtctc
     4801 gctctatgcc aatattggat tattgagcga tgttgccttt gctcagctgc acggcgccca
     4861 gggggtcgga ctctatcgca ccgagattcc gtttttgtcc catcgcgact ttccgagcga
     4921 agacgagcag tattcgctct atagcagggt ggttgaaggc atggccggaa agccggtcac
     4981 gatcagaacc ttggatatag gcgcagacaa gtatcccacc tacatgcgca gccccacggt
     5041 cgagccaaac ccctttttag gctggcgttc gatccgtatt tctctggaag tcgaggaaat
     5101 tttcaaaacg cagctgcgcg cggtcctccg tgtgggcgag ctcggccgcg tccgcttatt
     5161 gatcccgatg gtcacgagtc tcgaggaagt tttgaaggta aaggaaatcc tcgccgaggc
     5221 caaagccgaa ctcgatcatg aaggcacacc ctacgaccga caaatggagc ttggcatcat
     5281 ggtcgaggtt cccgccgccg tacaactcgc cgatcgcttt ctacgggaag ttgattttct
     5341 cagcatcgga accaacgatt tgattcagta cattctcgcg gtggatcgca gcaaccgcaa
     5401 ggttgcgagt ctttacgagc cgttgcatcc cgccgtgctc aaatcgctca gcacgacgat
     5461 tgacgccggg aagcggcaag gcaaacgggt cgggatgtgc ggcgaaatgg cgggcgatcc
     5521 gctctgcgct attctgctgc tgggaatggg tttagaggaa tttagcactg gctcgctcta
     5581 tattccggtc ataaaaaagg ccattcgctc gatcagctat cagagcgcca aagccagcgc
     5641 cgaaatcgtt ctcgccatgg atgcggtcgg ggagataaaa cgctatcttt tcgagcagat
     5701 gcggcaactc ggcatggttg agctactgga gatgtaccgt taagtttgaa agggttattt
     5761 ttcgctggtc gcgggttgaa aacgccttat atcagggtta ccttattatt cgaacgttcg
     5821 ttcgaaatgc tgaaaggaac tcctcatgac caaaatttcg acttttgacg actggatcga
     5881 ctatttccgc gagtggcagc gcgatatcgg ctacgaccct acactgcttg gcgactacaa
     5941 gtttgaaacc aagctcggcg aattgcactc acctgaaatc gaattcggcg actacaaagg
     6001 ccagcagaag tggcaacgcg tcacccagat cccgaatca
//
LOCUS       DASULZ010000500         4568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_61521, whole genome shotgun sequence.
ACCESSION   DASULZ010000500 DASULZ010000000
VERSION     DASULZ010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61521"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..1303
                     /locus_tag="VFY96_10220"
     CDS             215..1303
                     /locus_tag="VFY96_10220"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5606881.1"
                     /translation="MSEKKHKIIDGDGHVVEDHSAIVAHMPEMYRDRFGTSRGAYPPN
                     DHLHSANAHFLPPGAFAKVGREGWIDFMEDVGLTSAVLYPTNGLSFGRIVSLDWAIEL
                     ARAYNNWMHDEYLSKSSRFQALGLIPLQEPQEAVTELRRIVKELGFCGAMLPSTGVMG
                     GQNHLGDERYWPIYQEANRLECCIGIHGGVHDHMGLDDMTPYAPVNALGHPFGQMVNF
                     GGILFNGVFDKYPKVRFGFMEAGSGWFMNCIERFERAWNSHVQYDPRGRFLKLRDKES
                     ITDYIRRHIEEERIFVGVEGDELTLPFAVSIVGNKPFIFSSDFPHEVNNDTCKAELEE
                     LAENPRLSTEDKDAVRYRNAERFYGLPV"
     gene            1528..2349
                     /locus_tag="VFY96_10225"
     CDS             1528..2349
                     /locus_tag="VFY96_10225"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5606882.1"
                     /translation="MWQYAGMFLFGFAVSAFGTIVGFGGGVFMVPILTLVFGIPIQIA
                     IGCVILALFPSALISTVHNVRRGSVDFLAGILLEIPTVIGTILGAWLTDYLPTRQLEI
                     LFSLMVISAGSMMLRNNRRDRSSQLWFFTKLNKLPPRIVRDTATGSYEMSAVTVVIFG
                     LIAGMIAGLFGIGGGFIKGPLMVVGFGIPARIAAPTALFMIVITSAVGSVTHYLLGHI
                     HWQIGLFLVAAFTLGALVGNQKAGTIAEENLVGFIAGGLIAAGLAMAIFTVSQVK"
     gene            2425..2574
                     /locus_tag="VFY96_10230"
     CDS             2425..2574
                     /locus_tag="VFY96_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606883.1"
                     /translation="MNNDMCKAELEELDENQRLTEPDKDAVCYRNAERFDGLQADCLS
                     RFAHT"
     gene            2751..3596
                     /locus_tag="VFY96_10235"
     CDS             2751..3596
                     /locus_tag="VFY96_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005992133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5606884.1"
                     /translation="MPYNYIWLFPLGILIGAFGTLIGAGGGFILVPILLIVYPHEDTE
                     LITSISLAVVFFNALSGSWAYSRMKRIDYKSGIIFAIATVPGAILGAVSTAYVPRPVF
                     DLVFGILMIAAGIFLWLSAKDEHVHANAHLHQQTPGKKSSRLVLRDFIDADGVRYRYE
                     YNPVIGIVLSVFVGFLSSLLGVGGGFIHVPALSRLLNFPVHVATATSHFVLAIMALTG
                     TLVHIARGVFDHGVRRTAILALGVLLGAQIGARLSNRVGGKWIIRGLALALVFVGIRL
                     IWSEI"
     gene            3842..>4568
                     /locus_tag="VFY96_10240"
     CDS             3842..>4568
                     /locus_tag="VFY96_10240"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606885.1"
                     /translation="MKCQRFAALFALFVNLCLWTQQAYSQPLTNFRIANGTSGENPAV
                     LWVGVDQGFFRKHGLNVEVIYMRVGSLAASALVSGDVNAVLTSSNAVLNLAAGGLDVV
                     IIGALFQRPEGDFMARPELKKPEDLKGKLVAIQSIGGGGWANNMLAMDYLGLDPDRDN
                     IRFIVLGDQPSRIQALETGRAQASWMGSTFSAPLKKKGYTLMVDLSRAPIPYVGSSLV
                     VRKSAAHQDVKTYEALLKGTIDSL"
BASE COUNT          936 a         1254 c         1294 g         1084 t
ORIGIN      
        1 cacgaggtcg acgccgaaac ctgcaagcac gagctcgagg aattgcgcga gaacactcag
       61 ctaactgaag cggataaaga tgcgatcctg ttcggcaacg cgcaaagatt ttaccaattg
      121 agcctctcgt aaggagaggc tcatcctgag cgtcagcgaa ggatctattc agattcttcg
      181 gcttcgcccg agaatgacag ccatgaggaa tagaatgagc gagaaaaaac acaagatcat
      241 cgacggcgat ggccatgtgg tggaagatca ttccgccata gtggcgcaca tgccggaaat
      301 gtatcgcgac cgttttggga cgagccgcgg tgcctatccc cccaacgacc atctgcattc
      361 agccaacgca cactttcttc cgccgggcgc cttcgccaag gtcggacgcg agggttggat
      421 cgattttatg gaggacgtcg gcctcacgag cgccgttctc tatccgacca atgggctctc
      481 cttcggtcgt atcgtgagcc tcgactgggc tatcgagctg gcgcgcgcct ataacaattg
      541 gatgcacgat gaatatctta gtaagagcag ccgctttcag gcgttgggtt tgattccgct
      601 gcaagagccg caggaggcgg tgacagagct ccggcgcatc gtcaaggaac ttgggttttg
      661 cggtgccatg ctgccgagca cgggcgtgat gggcggacaa aaccatctgg gtgatgaaag
      721 atactggcca atttaccagg aagccaatcg cctggagtgc tgcatcggca ttcacggcgg
      781 cgtgcacgat catatgggcc tcgatgatat gacgccttat gcgccggtga acgcgctcgg
      841 ccatccgttc ggacagatgg tgaatttcgg cgggatcttg ttcaatggcg tgttcgacaa
      901 atatcctaag gtccgcttcg gtttcatgga agcgggctcg ggctggttca tgaactgcat
      961 cgaacgcttc gagcgcgcct ggaatagcca tgtgcagtat gatccgcgcg gtcgctttct
     1021 gaaactgcgc gacaaggaat cgatcaccga ctacatcagg cggcacatcg aggaggaacg
     1081 gatctttgtc ggagtcgaag gcgacgagct caccctccca tttgccgtca gcatcgtcgg
     1141 caacaagccg ttcatctttt cctcggattt tccgcacgag gtcaacaacg atacctgcaa
     1201 ggccgagctc gaagaactgg ctgagaatcc gaggctcagc accgaagaca aggacgctgt
     1261 tcgctaccgc aacgcggaac ggttttacgg attgccggtg tgacaccatc ctggaaatag
     1321 cggtccgatt tttctctctt ccatcgtaaa tttccggtgc gctcggaaga gacgctcggg
     1381 ttttgtatcc cgcgtcccgg gcaagccgaa gtacggaacc cggaaccgcg aggcattgtc
     1441 ccgtccgaca gatcacgcgc gacagggaat ctctgactga aatccggatg ccgacacgtc
     1501 taagcttctg cttctagatc gcttgctatg tggcaatatg cgggcatgtt cttgtttggt
     1561 tttgccgtca gtgccttcgg cactatcgtc ggttttggcg gcggtgtttt tatggtgccg
     1621 attctcacgt tggtctttgg catcccgatt caaatcgcga ttggctgtgt tatcctcgcc
     1681 ctattcccgt ccgccctcat ctcaacggtt cacaacgtcc gcagaggttc agtcgatttt
     1741 ctcgccggaa tcctgcttga gattccaacc gtcatcggta cgattctcgg ggcatggctg
     1801 actgattatc ttccgacccg gcagcttgaa atccttttca gcttaatggt aatcagcgcc
     1861 ggcagcatga tgttgcgaaa taaccgtcgg gatcgctcgt cgcagttgtg gtttttcaca
     1921 aagctcaata agcttccgcc tcggattgtc cgcgacaccg ccaccggctc ctacgaaatg
     1981 agcgcggtta cggtggtgat tttcggactc atcgccggca tgatcgcggg actctttggg
     2041 attggcggag ggttcatcaa ggggccgctt atggtggttg ggtttggaat tccggcgcga
     2101 atcgcggctc ccacggccct gttcatgatc gtcatcacca gcgccgtggg ctccgttacc
     2161 cattacctcc tcggccatat tcactggcaa atcggacttt ttctcgtcgc ggcgttcaca
     2221 ttaggcgccc tcgttggcaa tcaaaaggcc ggaaccattg cagaagaaaa cttggtgggg
     2281 ttcatcgccg gcgggctgat tgctgccggg ctcgccatgg ctattttcac ggtctctcag
     2341 gtgaagtaaa gcgcgaggcg caaatcttgc ccaacgtata ctagcatctc acgggaaccg
     2401 cggataataa ggataatcag cgaggtgaac aacgacatgt gcaaagccga gctcgaagaa
     2461 ttggacgaga accagcggct caccgagccg gacaaagatg ccgtgtgtta tcggaacgcc
     2521 gaacgctttg acggattgca agccgattgt ttgtcccgct tcgctcacac gtgagaggga
     2581 gaaatcaagc accgagaaca agtacgttga gtgcctgctg acgcgacatg ttgaattcgc
     2641 gtacaggcgt caatctattt tctcctccca aatcccgtgc taacttcgcg agcagctaca
     2701 tcaggatctg taacccttta cgaggtagtt ccgatgcctg tagctgcctc atgccttata
     2761 actatatctg gttgttccct ctcggcatcc tgatcggcgc cttcgggacg ttgatcggcg
     2821 ccggcggcgg gttcattctc gtgccgattt tgctgatcgt ttacccgcat gaagacaccg
     2881 agctgatcac aagcatctct ctcgcggtcg tattcttcaa tgcgctgtcc ggctcatggg
     2941 cgtatagccg gatgaaaaga atcgattata aatccggcat catcttcgcg atcgccactg
     3001 tgcccggcgc gatcttgggc gcagtctcca cggcgtacgt gccgcgcccc gtgtttgatc
     3061 ttgtttttgg tatcttgatg atcgccgcgg gaatattcct ctggttatcg gccaaagacg
     3121 agcacgttca tgcgaacgct catctgcacc aacaaacgcc gggcaaaaag tcttcgcgcc
     3181 tcgtgcttcg cgatttcatc gatgccgacg gggtgcgcta tcggtacgaa tacaatccgg
     3241 tcatcggcat cgtgctgagc gtcttcgtcg gttttctatc gagtctactc ggcgtcggcg
     3301 gcggcttcat tcacgtgccc gcgttatcgc ggttgctgaa tttccccgtc cacgttgcga
     3361 ccgccacttc ccattttgtc ctcgccatca tggcattgac gggaactctg gtgcacattg
     3421 cgcggggagt cttcgatcat ggagttcggc gcaccgcaat cctcgcgttg ggagttttgc
     3481 tgggcgccca gatcggcgca cgcctgtcga accgagtcgg cggcaaatgg atcatccgcg
     3541 gcctcgctct ggccttggtc ttcgtcggta ttcgcttgat ctggtcggaa atttgaccgc
     3601 gggagcaggc gtccgtcgtg aaatcgagac tgttgtcgca atgcaccgat tgcgcgatag
     3661 ttgtcatctc gagctccgcg agaatctcgc gcttccatac gaagaaagat ttctcgccgg
     3721 agtttatcct gtgagtgaaa tccaaggaac tcgaaatgac aattgcggca cggttctcga
     3781 ctgcggagag ttagggtcgg tgtgatattt tgctcgtttc ttccgggcca ggagagttga
     3841 aatgaaatgc caacgatttg ccgccctttt cgctttattt gttaaccttt gtctttggac
     3901 gcagcaggcg tacagtcaac cgcttacgaa ctttcgcatc gctaacggca ccagcggcga
     3961 aaaccccgca gtgctttggg tcggcgtcga ccagggcttt tttcgcaagc acggtctcaa
     4021 tgtcgaggtc atctacatgc gggtggggtc ccttgccgct tcggcattgg tatccggtga
     4081 tgtgaatgca gtgctcacca gttcgaatgc cgtgttgaac cttgccgccg gtggactgga
     4141 cgtcgtcatt atcggcgcgc tttttcaacg gccggaaggg gatttcatgg ctcgcccaga
     4201 attgaaaaaa cccgaggacc tgaagggcaa attggtcgca atccagagta tcggcggcgg
     4261 cgggtgggcg aacaacatgc tggcgatgga ttacctcggt ctcgacccgg accgtgacaa
     4321 tattcgcttt atcgtattgg gcgatcagcc gtcccgaatc caagctcttg aaacgggacg
     4381 cgctcaggcc tcctggatgg gctcgacctt cagcgcgccg ctgaagaaaa aaggctacac
     4441 actgatggtg gatctcagcc gtgcgccgat tccgtacgtc ggttcgtcct tggtggtgcg
     4501 gaaaagcgcg gcgcatcagg atgtaaaaac ctatgaggct ttgctcaagg gaacgatcga
     4561 ttcgctgc
//
LOCUS       DASULZ010000501         5146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_669621, whole genome shotgun sequence.
ACCESSION   DASULZ010000501 DASULZ010000000
VERSION     DASULZ010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669621"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..754)
                     /gene="phoU"
                     /locus_tag="VFY96_10245"
     CDS             complement(68..754)
                     /gene="phoU"
                     /locus_tag="VFY96_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX5606886.1"
                     /translation="MPNEHTDKKYEEELKKLREEILYMGGLVEDQIQKAVKSLVDRDS
                     ELAKIIIERDHEVNRLDVDIDDICIRLLALHQPAGRDLRFITTGLKITTDLERIGDMA
                     VNMCERALELNLEPQLKPYIDIPRMARIAQRMIRESLDAFVREDTELALRVCKADDEV
                     DQLNAQIFRETLTFMIENVQTISRATKISSVSKYLERIADHATNIAEMVIFMVKGKSI
                     RHLKELPPSV"
     gene            complement(768..1514)
                     /gene="pstB"
                     /locus_tag="VFY96_10250"
     CDS             complement(768..1514)
                     /gene="pstB"
                     /locus_tag="VFY96_10250"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX5606887.1"
                     /translation="MRVEDLSVYYGKVQAVKQVTMSVRTQKVTALIGPSGCGKSTFIR
                     CLNRMHELTPGGRVEGKVLLDEQDLYGTGVDPVDVRRRVGMVFQKPNPFPNMSVRDNA
                     IAGFKLSGVRDRKLLDEVVEKSLRDAALWDEVKDNLQKSGASLSGGQQQRLCIARALA
                     VTPEVLLMDEPCSALDPIATAKIEELIHELKEQVTIVIVTHNMQQAARVSDFTAFFYL
                     GELVEFGETGKLFTAPEKRQTEDYITGRFG"
     gene            complement(1609..2451)
                     /gene="pstA"
                     /locus_tag="VFY96_10255"
     CDS             complement(1609..2451)
                     /gene="pstA"
                     /locus_tag="VFY96_10255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX5606888.1"
                     /translation="MNSHYRYRKFIQSATWVAMLCCLSISVFFLMAILGYVIVNGASY
                     INWEFLTALPVPAGESGGGIANALVGSLMIVVVASLMAIPIGIGAAIFINEFPSHGTG
                     RTIRFLAEVLTGVPAIVVGLFAYDLIVRSWGGFSAFSGSVAYAFIMVPIILITTHEAL
                     RLVPDSLREASLALGIPKWRTILGVVLPVSSRALITGVVLATSRALGEAAPMLFTAFG
                     NPFWNLNMAKPMATVPTLIYTYATGPYDDWHQKAWAASFVLLVVVLFTSVLTRAFLRG
                     KANE"
     gene            complement(2483..3490)
                     /gene="pstC"
                     /locus_tag="VFY96_10260"
     CDS             complement(2483..3490)
                     /gene="pstC"
                     /locus_tag="VFY96_10260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX5606889.1"
                     /translation="MRSDPQATFESLSQGLPSAEGRSISARRQVINIGDRVFYMITRS
                     LGLFVIVLAVLLVWNLVDGAWEAIRRFGLGFLIGTTWDPVTRVFSALPTIIGTLVKGF
                     IGVSLAVPISIGSAIFLAFYTPKWLRPVLSYPIELLAGIPSIIFGMWALFAMVPVVRV
                     FQMWLKANFGWFFLFDGPAVYGVGVLAGSLILAIMITPIITSISKEILLTVPRGQIEG
                     MLALGATQWEAIWKVALPYSRIGLFGAIILGLGRALGETMAVTMVGGNAFALIHSLFD
                     PVHSMASQIASEFTEATYSLYVSSLIYVGLILFGITILISLLAQLLIWRMAKGRLAIL
                     E"
     gene            complement(3505..4566)
                     /gene="pstS"
                     /locus_tag="VFY96_10265"
     CDS             complement(3505..4566)
                     /gene="pstS"
                     /locus_tag="VFY96_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015235021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEX5606890.1"
                     /translation="MDLKKITVAVLLIALSAVSGEALAGCPTKGVKELTGAGATFPYP
                     LYSKMFDEYNKACGVKVNYQSIGSGGGIQQLKEQTVDFGASDGIMDEKQRAEAKGGAV
                     LHIPTVAGAEAIVINLPGIQRSQLKLTPEVLADIYLKKITNWNDKRIQAINSGLKLPD
                     LTIAVVHRSDGSGTTFIFTNYLSKVSPEWKDKVGFATSVNWPGDVGGKGNEGVANSVK
                     QIPGGIGYVELAYAKQSKMVWAQLQNKAKKYLDPSLEGAAAAADVANLPDNMEVMITD
                     SSNPNAYPISGFTWLLVYANQTDAAKGDAVAQLSWWMLHEGQQYALPLEYAPLKGAAI
                     KKAEALVKKIKVNGAVALK"
     gene            4887..>5146
                     /locus_tag="VFY96_10270"
     CDS             4887..>5146
                     /locus_tag="VFY96_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HEX5606891.1"
                     /translation="MAGGMKMFGKHRLPGRLFIVEGIDGSGKSTQLMLLQKWLESEGY
                     VVYSSEWNSSPLVHDVTKRGKKKKMLTPMTFSLIHATDFADRT"
BASE COUNT         1219 a         1404 c         1333 g         1190 t
ORIGIN      
        1 tgaccagctt gcgaatatcc ggctcatctt cgaccaccaa aatttttttt ccggccttca
       61 tacgttatca aaccgagggg ggcaattcct tcaagtggcg aatgctcttg cctttgacca
      121 tgaaaatcac catttccgcg atattggttg cgtggtcggc gatgcgttcg aggtatttcg
      181 aaaccgagct aattttggtc gctcggctga tggtttgcac gttttcgatc atgaaggtga
      241 gggtttcacg gaagatttgc gcgttgagct gatcgacttc atcatcggct ttgcacaccc
      301 tcagagcgag ctccgtgtcc tcgcgcacga acgcatcgag actttcgcgg atcatccgct
      361 gcgcgatgcg cgccatccga ggaatatcga tgtagggttt caactgaggc tctaggttaa
      421 gctccagcgc tcgctcgcac atattgacgg ccatatcgcc gatgcgctcg agatcggtgg
      481 tgatcttgag accggtggtg ataaatctga ggtcacggcc ggcgggttgg tgcagggcga
      541 gcaggcggat gcagatatcg tcgatgtcga cgtcgagccg gtttacttca tgatcgcgct
      601 cgataatgat cttggcgagc tcggaatcgc gatcgaccag cgacttcacc gctttctgaa
      661 tctggtcctc gacgaggcct cccatataga ggatttcctc gcggagcttt ttcaactctt
      721 cttcgtactt tttgtcggtg tgctcattag gcatcgtatc gcctccatca gccaaaacgg
      781 ccggtgatgt aatcttccgt ctgccgtttc tccggcgcgg tgaagagctt tccagtctcg
      841 ccgaactcga ccaactcgcc gagatagaag aaggcggtaa agtcggagac gcgcgccgct
      901 tgctgcatat tgtgggtgac gatcacgatg gtgacctgct ctttgagctc atggataagc
      961 tcttcgattt tggcggtggc gatggggtcg agagccgagc agggctcgtc catcaacagg
     1021 acctcaggag tgacggcaag cgctcttgca atgcagagac gctgctgctg gcctccggaa
     1081 aggctcgcgc ccgacttttg taagttgtct tttacctcat cccagagggc ggcgtcacgc
     1141 aggctctttt cgacgacttc atcaagaagt ttgcgatcgc gaacgccgct caatttgaat
     1201 ccggcaatcg cattgtcgcg cactgacatg ttcggaaacg gattgggctt ctgaaaaacc
     1261 atgccgacgc gccggcgcac gtcgacagga tcgacgcccg tgccgtagag atcctgctcg
     1321 tcaagcaaaa ccttgccttc gacccggcct ccgggagtca gctcgtgcat acggttcaag
     1381 cagcggataa aagttgattt gccgcagccg gacggcccga tcagggcggt aaccttctgc
     1441 gtccggaccg acatagtcac ttgtttgacc gcttgaactt tgccgtaata caccgacagg
     1501 tcttcgacgc gcatgcgcac gccgctgtca gccttggccg cctcggaggc actgtcaagg
     1561 gctacgtcaa ggtcacgggt tttcacatga ttcatcgcat aggtctcgct actcattggc
     1621 tttgcctctt aaaaaggccc tggtcaaaac gctggtgaag agcacgacta cgagcaatac
     1681 gaacgaagcc gcccaagctt tttgatgcca atcgtcataa ggcccggtgg cataggtgta
     1741 aataagtgtc ggtacagtgg ccatcggctt tgccatatta aggttccaga atggattccc
     1801 gaaagcggta aacagcatcg gagccgcttc tcccagcgca cgcgatgttg ccaacaccac
     1861 gccggtaatg agcgcccgag aggaaacagg caaaaccact ccgagaatgg tgcgccattt
     1921 gggaatccca agagcgagag aagcctcacg tagggagtcc ggcaccagac gcaaagcttc
     1981 atgggtcgtg atcagaataa tgggtaccat aatgaaggca tacgctaccg acccggaaaa
     2041 agcggagaag ccaccccagg acctgacaat cagatcatag gcaaacaacc cgacgacaat
     2101 cgccgggaca cccgtcaaga cctcggctaa aaatcggatc gtgcggccgg tgccatggct
     2161 gggaaattcg ttgatgaaaa tcgccgcgcc aataccaatc ggaatcgcca tcaaactcgc
     2221 taccaccaca atcatcaggc tgccgaccaa ggcattcgca ataccgccac cagattcacc
     2281 agcgggtacc ggcagtgcgg tgagaaattc ccaattgata tagctggcgc cgttgacgat
     2341 gacatatccc aatatcgcca tcagaaagaa cacactgatg ctgaggcagc agagcattgc
     2401 aacccaggtc gcgctctgga tgaatttacg gtagcgataa tggctgttca atttcgtctc
     2461 ctgtttacgg agctgcgctc gattattcca aaatcgcaag tcttcctttt gccattcgcc
     2521 agatcagtag ctgggcgagc aagctaatca aaatcgtaat cccaaacaga atcaggccga
     2581 cgtatatgag agatgagacg tatagactat acgtcgcctc ggtgaactcg ctggcgattt
     2641 gactggccat actgtgcacc ggatcgaaca gcgaatggat cagcgcaaac gcgttgcctc
     2701 cgaccatggt taccgccatc gtttctccga gagcacgacc cagtccgagg attatggcac
     2761 cgaaaaggcc aatgcgtgaa tagggtaagg ccaccttcca gatcgcctcc cattgggtcg
     2821 cgcccaacgc gagcattccc tcgatctgcc cacggggtac ggtaagcaat atttctttcg
     2881 atatagaggt gattatgggg gtaatcatga tggctaaaat caggctgccg gcaagaactc
     2941 cgactccata caccgctggc ccatcaaaga gaaaaaacca gccgaaattc gctttcagcc
     3001 acatttggaa cactcggact accggcacca tggcgaacaa cgcccacatc ccaaaaatga
     3061 tgctggggat gcccgccaat agctcaatcg ggtaagaaag cacaggacgg agccatttcg
     3121 gagtatagaa ggcgagaaaa atcgctgagc cgatactgat cggaacggcc aaggagacgc
     3181 cgatgaagcc ctttaccagc gtcccaatga ttgttggcaa tgcgctaaac acacgggtca
     3241 ctggatccca ggtcgttcca atgagaaagc ccagaccgaa tcgtcggatc gcttcccacg
     3301 cgccatcgac aagattccac accaacaaca ccgccagcac gatcacaaac agtccaagag
     3361 atcgcgtaat catgtaaaag acgcggtcgc cgatattgat aacctgccgc cgagcgctga
     3421 tggaccgtcc ctcagcgctc ggcagcccct ggctcagcga ttcaaaggtc gcttgtggat
     3481 cgcttcgcat tggtgtctcc gccactactt cagagcaaca gcgccgttga ctttgatttt
     3541 cttgaccaaa gcctcggcct tttttatcgc ggcgcctttg agcggggcat actccagtgg
     3601 cagggcatac tgttgccctt cgtggagcat ccaccagctc aattgtgcta ccgcgtcgcc
     3661 ttttgccgcg tcggtttgat tcgcgtatac cagcaaccag gtaaatccgc tgatagggta
     3721 cgcgttggga ttgctgctgt cggtgatcat gacctccatg ttatccggca gatttgcgac
     3781 gtcagccgcg gcggctgcac cttcgaggct cgggtccaga tactttttgg ctttgttctg
     3841 tagctgtgcc cagaccattt tgctttgttt agcataagcc agttcgacat aaccgatgcc
     3901 gccgggaatt tgcttgactg agttggcgac accttcattg cctttgccgc ctacatcgcc
     3961 gggccagttg accgaagtcg caaacccgac tttatctttc cattccggac tcaccttgga
     4021 gagatagttg gtgaaaataa aggtcgtgcc ggaaccgtcg gatcgatgga caactgcaat
     4081 tgtcaagtcc ggtagcttga gaccggagtt aattgcctgg atccgtttat cgttccaatt
     4141 agtaattttt ttgaggtaga tgtccgcgag aacttctggc gtgagcttca attggcttcg
     4201 ttggattcca ggcagattaa taacaatcgc ctcggcacca gccacggtgg gaatatgaag
     4261 aaccgcgccg cctttagctt cggccctctg cttctcgtcc atgattccgt ccgaagcgcc
     4321 gaagtctacg gtttgttcct taagttgttg aatgcccccg cccgagccaa tggattgata
     4381 gtttaccttt acgccgcatg ccttattata ttcatcgaac atctttgaat agagcgggta
     4441 cggaaaagtc gctcccgctc ctgtcaactc tttaacccct ttggtcggac atcccgccag
     4501 cgcctcacct gaaacagccg atagcgcgat taacaagacg gccaccgtta ttttctttag
     4561 atccataatt cctcctaatt attatgttta gagaccaaac gttacgaaca ggttacgaac
     4621 aggttaacgt tacaagaaag aaacacggca aagagatttg cccttcattt tccgctccga
     4681 caattactga gtctgacgat tcggttatct agctctcgta aatgaactat taaggaacgg
     4741 taaagttcgt gttaaacaga aactgaaggc caagtattcc cagtcattta attgaaatct
     4801 aagatctcgc tggagcctct agccaagccg ttgcaggctc tgttagtctg acagcttcat
     4861 tgacccccct taaggagaag tttgttatgg ctggtggcat gaagatgttc ggtaaacatc
     4921 gtcttcccgg aagactgttt atcgtcgagg gtatcgacgg ttccggcaag agcacccagt
     4981 tgatgttgct gcagaagtgg ctcgagtcgg agggctatgt cgtttactcg agcgaatgga
     5041 attcttcgcc tctggtccat gacgtgacca aacgcggcaa gaaaaaaaag atgctgaccc
     5101 cgatgacgtt tagcctgatt cacgccacgg actttgccga tcgcac
//
LOCUS       DASULZ010000502         2511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1074169, whole genome shotgun sequence.
ACCESSION   DASULZ010000502 DASULZ010000000
VERSION     DASULZ010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074169"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VFY96_10275"
     CDS             complement(<1..320)
                     /locus_tag="VFY96_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606892.1"
                     /translation="MLKTRCKVFSVSLARAALFTTQALIISICALPLATGVSFFISHA
                     HAQTAVKTEKITATIALPGVQVESLNPYAHSTTQIYPTWKHVIEPLFEWSWSKRQLVP
                     ILAES"
     gene            623..1015
                     /locus_tag="VFY96_10280"
     CDS             623..1015
                     /locus_tag="VFY96_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606893.1"
                     /translation="MKKHKFLFAGILFLFVLGVSGLAAAQDSPRKDDGGARVQGKAGD
                     HSFDVQVQGGESRRDSRPPRESPAIDNSRPGPQGRQGEPGPQGPAGPAGPSGGTILGM
                     DSTLALFVGLAVFAVVIVGIVAASRGRE"
     gene            complement(1130..>2511)
                     /locus_tag="VFY96_10285"
     CDS             complement(1130..>2511)
                     /locus_tag="VFY96_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606894.1"
                     /translation="TFLLPVLIRNILIGISMLKSGLFSSAALLFAVSVFLLASPRPMQ
                     KWPWRAESEATDFPYLQRGNEIQNEFKSYSDALADYYYRLTAALRSELPELVADLRAP
                     DTRRSGYQILPQVTPDDLTETPAPGQSGYSWPWAERLIGRARHQLAHFEMQLRRTQSL
                     DSARRRAALEKLARDYGGLRQDLANLEAHVQYNRFWQSVIAADRASYDREMLLYRDVL
                     AREQVRDELRYFDGGFANAPVALQPAFGVLSLANFRDSLEKREALLDERIDSALGQIT
                     TPAFISLERRAHEWIFRVPLYTDVKEQNFIAAAKRMIEAVWRLAGNSNTYRVEIEISY
                     VPTELLYADSEKPAPGEKLDVKEHLRHFPANAAILTTGALTTHVEKNAIVLGPKSISP
                     RVLAHEFGHILGFRDRYIRGYRDLGKDGFEVLEAIADPGDIMGAPATGSVLATHFDAL
                     IQAMSRAQK"
BASE COUNT          567 a          663 c          731 g          550 t
ORIGIN      
        1 gattccgcga gaatcggcac gagttgcctt ttactccagc tccattcgaa caatggctcg
       61 atgacatgtt tccaggtcgg atagatttgc gtcgtcgaat gggcataggg attcagactt
      121 tccacctgaa cgccgggcag agcgatggtc gcggtaatct tctcggtttt gaccgcggtt
      181 tgcgcgtgag cgtgagaaat gaagaaactt acgccagtag ccaatggaag cgcgcagatg
      241 cttatgatta gcgcctgggt agtaaataac gcggcgcgcg caagcgaaac agaaaaaact
      301 ttgcagcggg ttttcagcat tacggtgctc cctcgaattg aacgaaccga gttgacttat
      361 gaacgtgcca cggatcgaag tcaacattaa acgcatttcg gtttacctgc ctagaacagg
      421 tgaggaatgc gtagcggcgc gatggttcgc gtatgatttt ggctattatg cgagcagtta
      481 aatccgtgca tcggacgcgc gccgattttc gctatttcat tgggcgcacg gcggcgcgag
      541 atactaactc gttgatattt ttgaccctgt tctacagggc atagcttttg ctcaaaaagc
      601 taaaaacgag aggaggtgct ttatgaaaaa acacaaattt ttattcgccg gcatcctgtt
      661 cctctttgtg cttggggtgt ccggtttggc cgccgctcag gattccccgc gaaaggacga
      721 tggtggtgct agggttcaag ggaaagcggg cgatcacagc ttcgacgtgc aagttcaagg
      781 gggagaatct cgacgcgact caagaccgcc acgtgagtca cctgcgattg acaacagcag
      841 accgggaccg caggggaggc aaggagaacc cggacctcaa gggccggcag gacccgcagg
      901 tccgtctggc ggaacgatat tgggcatgga ttccactctg gcgctattcg tcgggctggc
      961 tgttttcgca gtggtaattg tcggaatcgt cgctgcgtcc cggggcagag aatagcaacc
     1021 caagccgcaa aacctttcgg cggattttcg atatcggcat ttgctattgc cgaatcgaga
     1081 tccgccgaag ttttaattat cgaccgctcc gattagagaa tattggccat catttttgcg
     1141 cgcgcgacat ggcctgaatc agagcgtcaa aatgcgtggc taagaccgag cccgttgcgg
     1201 gcgcgcccat gatgtcgcca ggatcggcga tggcttccaa aacctcgaag ccgtcttttc
     1261 cgagatcccg gtaaccgcga atatatcggt ccctgaatcc gagaatatgc ccaaactcgt
     1321 gcgccagcac gcgcggcgaa atagacttcg gcccgagcac gatcgcattc ttctcaacat
     1381 gggtcgtcaa tgcgccggtg gtaaggatcg ccgcgttcgc cggaaaatgt ctcagatgct
     1441 ctttgacgtc aagtttttcg cccggcgccg gcttttcgga gtccgcatac agtagttcag
     1501 ttggcacata tgaaatctcg atctcgaccc gatacgtatt gctgttgccg gcgaggcgcc
     1561 agaccgcttc gatcattcgc ttcgcggcag caatgaaatt ttgctcttta acatcggtgt
     1621 agagcggcac gcggaaaatc cactcgtgtg cgcgccgctc caagctgatg aatgcgggcg
     1681 tggtgatttg cccgagagcg ctgtcgatgc gttcgtcgag cagcgcttca cgtttttcca
     1741 agctatcccg gaaattcgca agactcagca cgccgaaagc aggctgcagc gctaccggcg
     1801 cattggcaaa tccgccatcg aaatagcgta attcgtctcg aacctgttcg cgcgcgagga
     1861 cgtcacggta aagcagcatc tcacggtcgt agcttgcgcg atctgcagca ataaccgatt
     1921 gccagaagcg attgtattga acatgcgcct cgagattggc caggtcctga cggagcccac
     1981 cgtagtcgcg cgcaagtttt tcgagcgcgg cgcgtcggcg cgccgaatcg agtgattggg
     2041 tgcgacgcag ttgcatctcg aagtgcgcca gctgatgacg tgcgcgtcca atcagccgct
     2101 ctgcccaggg ccaactatac cccgactggc cgggagccgg tgtttctgtc aggtcatcgg
     2161 gagtcacttg aggcaaaatc tgatagcccg accttcttgt gtcgggggcg cgaagatccg
     2221 cgacaagctc cggcagttcc gaccgaagcg cggctgtgag ccggtaatag taatcggcaa
     2281 gagcgtcgct gtacgactta aactcattct gtatctcgtt gccgcgctga agatatggga
     2341 agtccgtcgc ctcagattcg gcgcgccacg gccatttttg catgggccgc ggcgaggcga
     2401 gcaaaaaaac gctgacggcg aagagcagcg ccgcactaga aaacaatcca gatttcagca
     2461 tggaaatgcc gatcaggata ttccggatta ggacggggag caagaaggta a
//
LOCUS       DASULZ010000503         2377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1380570, whole genome shotgun sequence.
ACCESSION   DASULZ010000503 DASULZ010000000
VERSION     DASULZ010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380570"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="VFY96_10290"
     CDS             complement(<1..869)
                     /locus_tag="VFY96_10290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5606895.1"
                     /translation="MKLKRPLLIAGLLLSVGGAGFTYWRIGSTPKEPPFLTVPVSKSN
                     VRQVVSSTGTLQAVTTVLVGSQVSGTIAKLSADFNTKVTTGQVVAVLDQSKFKAKLEE
                     TRANVLSAQANLAKAKVALEDAERTLKRTKELQGRELVAQSELDAAQTTYDAARSQVN
                     VMQAQVNQAQASMNQASIDLDYTVIRSPVDGIVISRSVDVGQTVAASLQAPTLFTIAN
                     DLTKMEVHTNVDEADVGNIHEGQDVSFTVDAHPNRRFRGNVHQVRNAPQIIQNVVTYD
                     AVVRINNKELLLKP"
     gene            complement(866..1342)
                     /locus_tag="VFY96_10295"
     CDS             complement(866..1342)
                     /locus_tag="VFY96_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606896.1"
                     /translation="MSQLLGNELTPELLARLNGSAIESHEGKIIPIFTNDENGWPHPA
                     LLSYYEIVARDRSSLEMALWKTSSTANNLRRTGKVTLMITDVATNYYLKGSVRELQYE
                     MIGAAPVSRFRVAIDQVIEDQEPNAEITTGLTYRRMTERDPNDFSVKVFRLLHADS"
     gene            complement(1377..1850)
                     /locus_tag="VFY96_10300"
     CDS             complement(1377..1850)
                     /locus_tag="VFY96_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5606897.1"
                     /translation="MPTDLKQQILEYLRSHNTMTLATTQGDLPWAATVFFASEDFRLY
                     FFSAPESRHCQNLAVNPRVAVTIQEDYQDWRQIKGIQIEGRVKLVDGVIEKAKAMAIY
                     ARKYPGIIKLFTDPASGIFHKAFLKVKFYCVETERIFFINNEQGFGKRQELVLDR"
     gene            complement(1897..>2377)
                     /locus_tag="VFY96_10305"
     CDS             complement(1897..>2377)
                     /locus_tag="VFY96_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEX5606898.1"
                     /translation="RDLTVPVIVKEVGNGISVDVAKRLQRAGVRAIDVAGKGGTSWFA
                     VEAQRAAKTGQRADDTFADWGIPTEEAIVSVRQALPEMPLIASGGIRSGLDIAKSIAL
                     GADLAALGQPLLASALDSTAKVVEFLSRVIQELKISMLCVGAANLAALRRTPLTRLP"
BASE COUNT          511 a          685 c          637 g          544 t
ORIGIN      
        1 ggcttaagca ggagctcttt attgttgatc cgcaccaccg catcgtaagt tacgacgttc
       61 tgaatgattt gcggcgcgtt tcgcacctgg tgcacgtttc cgcggaaccg tcgattagga
      121 tgggcatcca cggtaaacga aacgtcctgc ccttcatgaa tgttcccgac atccgcctcg
      181 tctacattgg tatgcacttc cattttggtg aggtcgttgg cgatcgtgaa cagtgtcggc
      241 gcttgcagcg aagcggctac cgtttggccg acatcaacgc ttcgcgagat aacgattccg
      301 tcaacgggcg aacgaatcac agtgtagtcc agatcgatgg aagcttgatt catcgaggct
      361 tgggcctgat tcacctgcgc ttgcattacg ttcacctgcg aacgtgcagc gtcatacgtc
      421 gtttgcgccg cgtcgagctc gctctgcgcc accaactcac gcccttgtaa ttccttggtc
      481 ctcttgaggg tgcgctcagc gtcttcgagc gcaactttcg ctttcgcaag attggcctga
      541 gccgaaagca cgtttgcgcg ggtctcctcc agcttagcct tgaacttgga ctgatcaaga
      601 actgccacca cctgcccagt ggtgactttc gtattaaaat ccgcgctgag cttagcaatc
      661 gtgccagaaa cctggctgcc gacgagaacc gtggttaccg cttgcaacgt tccggtggag
      721 gaaactacct gacgaacatt gctcttgctg acgggcaccg taagaaaggg aggttcctta
      781 ggcgtagaac cgatgcgcca ataggtaaat ccggctcccc cgaccgacaa gagcaaaccg
      841 gcaatcagga gcggcctttt cagcttcatg aatccgcatg caatagccga aacactttca
      901 cggaaaaatc gttcgggtca cgttccgtca tacggcggta ggttaagccg gtggtaatct
      961 cagcgttggg ctcctggtct tcaatgacct ggtcaattgc gacacgaaac cgcgataccg
     1021 gcgcagcccc gatcatctcg tattggagct cgcgcacgct gccctttaga tagtagtttg
     1081 tcgcaacatc ggtaatcatc agcgtgacct tgcccgtgcg gcggagattg ttcgctgtcg
     1141 aactcgtctt ccacagagcc atctccagcg acgagcgatc ccgcgcaacg atctcatagt
     1201 aggacaacaa agcgggatgt ggccagccgt tttcatcatt ggtaaagatc ggaataatct
     1261 ttccttcgtg actttcgatc gccgagccat tgaggcgcgc gagcagctcc ggcgttaatt
     1321 catttcctag caattgcgac atttcggatc tcctcgccgg tgcacttata gccgatctat
     1381 cggtcgagca ccagttcttg tcttttgcca aacccctgct cgttattgat gaaaaaaatc
     1441 ctctcggttt cgacgcagta gaacttgacc ttgagaaaag ccttatgaaa gataccgctg
     1501 gcgggatccg tgaagagctt tatgatgcct gggtacttac gggcatagat cgccatcgcc
     1561 ttcgccttct cgataacgcc atccacgagc ttcactcgtc cttcgatttg aatacctttg
     1621 atctgccgcc aatcctggta atcctcctga atcgttactg cgacacgcgg attgacggcg
     1681 agattttggc agtggcgcga ctcgggcgcg gaaaaaaaat acagacggaa atcttcgctg
     1741 gcaaaaaaaa ccgtcgcggc ccagggcaga tcgccctggg tagtcgccag agtcatcgtg
     1801 ttatgggagc gcaggtactc caggatttgc tgctttaaat cggtaggcat gtgcagtcag
     1861 cagaaccgcg ccgcatcagg gcggacattg agagcgtcaa ggcagccgcg ttagtggcgt
     1921 ccggcgcagc gccgcgagat ttgccgctcc tacgcaaagc atgctgattt taagctcctg
     1981 aataacccgc gaaagaaatt ctacgacctt cgcggtcgag tccaaagccg acgccaaaag
     2041 cggctggccg agtgcggcga gatcagcccc gagcgcaatc gatttcgcga tatcgaggcc
     2101 gctgcgaatg ccgccggacg caatcagggg catctccgga agcgcttggc ggacgctgac
     2161 aattgcctcc tctgtcggta ttccccagtc ggcgaatgtg tcatcggccc tttgtcctgt
     2221 cttagccgcg cgctgtgctt ccacggcaaa ccaagaggtt ccgcctttgc ccgcaacatc
     2281 gatcgcgcga accccggcgc gttgtaaacg cttggcgaca tcgacggaaa tgccattgcc
     2341 cacttccttg acgatcaccg ggacggtcag gtcccgg
//
LOCUS       DASULZ010000504         2623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_770669, whole genome shotgun sequence.
ACCESSION   DASULZ010000504 DASULZ010000000
VERSION     DASULZ010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770669"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..389)
                     /locus_tag="VFY96_10310"
     CDS             complement(72..389)
                     /locus_tag="VFY96_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606899.1"
                     /translation="MIKGLLEGIAFCLSPRNKGAVITTIMKRLKLNDSAAAEEGYLDV
                     INGVERKPFPTIQGLSNIQRLMKLRSPSIDRLRLEDLIDDRILRRLDESGFIDALGKE
                     YPA"
     gene            complement(386..877)
                     /locus_tag="VFY96_10315"
     CDS             complement(386..877)
                     /locus_tag="VFY96_10315"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606900.1"
                     /translation="MLVAALTAGDVQAAYTGGTTVLPAVAGGSDLKIVMTLTNRLGYA
                     VVAAPSIKSAKDLHGKKSAYKRSPVRFGWAPCSRSSTWAWIRSATTSFFLVVGDQTVA
                     TQVLETGQIDMTVLDGSSAAGLSKKVSTFSPSFRRRTSRFLLRAWLPRAACCGSNARP
                     LRA"
     gene            complement(868..1056)
                     /locus_tag="VFY96_10320"
     CDS             complement(868..1056)
                     /locus_tag="VFY96_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606901.1"
                     /translation="MLLVLVAAMLMFTCCPNSHGAAPTRKIVIGHAAMNARVAPLWVA
                     QDHGTMPTQFSFAKRLCS"
     gene            complement(1092..1862)
                     /locus_tag="VFY96_10325"
     CDS             complement(1092..1862)
                     /locus_tag="VFY96_10325"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5606902.1"
                     /translation="MKLKEQVALITGAGRNIGKAMAKLFASEGAKIAVAEVHEGRGQA
                     VVDELNRADHEAMLVLGDISKSDDVQAMVKKVVARFGAIDILVNNAALTDHATILDSK
                     EEEFDKVIAVSLKGPYLMTKYVADEMRKQGRGGKIVNFGSTSGMIGRFDGIAYAAAKA
                     GVINMTRAMAVQLAQYKIRVNCVVPNRSGSPVGYDDDVEGNRTFQNLAGRLGTAEDQA
                     KAALFLVSEESDFIYGHPLVVDGGVMATANFPREQFKN"
     gene            complement(2138..>2623)
                     /locus_tag="VFY96_10330"
     CDS             complement(2138..>2623)
                     /locus_tag="VFY96_10330"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX5606903.1"
                     /translation="EAIAQSRGVVGGPWLALLHSPELAQRTMQLGSYIRFESTLDHKV
                     VEFTALVAARELECKHEWAAHIMHGQKAGIPMETIRAVYQKEGTEKFSSEDAQIVSFV
                     REMIHNHRVSESTFQAIYGRLGEKGMVELTATIGYYAMLACTLNTFDVYTFTPPEDLQ
                     L"
BASE COUNT          547 a          805 c          685 g          586 t
ORIGIN      
        1 gaatgccgac agaccgaacc atctgatcgc cgctccgccg acgtagaagt gaaattgaga
       61 actcaagctt atcaagcagg gtactctttc cccaatgcgt cgataaaacc gctttcatcg
      121 aggcggcgca gaatccgatc atcgatgaga tcctccaacc taagcctgtc gatactcgga
      181 ctgcgcaatt tcattagacg ttgaatattg ctcaggcctt gaatggtcgg aaacggtttg
      241 cgctcgacgc cgttgatcac gtcgagatag ccctcctcag ccgccgcgga gtcgttgagc
      301 ttgagccgct tcatgatggt cgtgatcacc gcgcctttgt tgcgcggcga caggcaaaac
      361 gcaattcctt cgagcagtcc tttgatcaag ctctcagcgg tctcgcgttg ctcccgcagc
      421 aggctgctct tggcaaccag gccctgagaa gaaaacggga tgttcgcctt cgaaagctcg
      481 gcgagaatgt tgaaaccttt ttgcttaagc cggcggctga agacccgtcg agcaccgtca
      541 tgtcgatctg gccggtttct aaaacctgcg tcgccacagt ctgatcgccg accacgagaa
      601 aaaatgatgt tgtcgcgctg cggatccagg cccaggtgct cgagcgcgag catggcgccc
      661 atccaaaccg taccggcgag cgtttgtacg ccgacttttt gccgtgtaaa tccttagcgc
      721 tcttgatgct cggggcggcg acgacggcgt agccgagccg attggtcagc gtcataacga
      781 tcttcaggtc cgagccgccc gccaccgccg gcaacaccgt cgtgccaccg gtgtaggccg
      841 cttgcacatc accggccgtg agcgcagcta cgagcacagg cgcttggcga atgaaaattg
      901 cgttggcatt gttccgtggt cctgggcgac ccagagcggc gccacccgcg cattcatcgc
      961 cgcatgaccg atgacgattt tgcgagtcgg cgcggcacca tgactgttag gacagcaggt
     1021 gaacatcaac atcgccgcga ccagcacaag cagcatgttt tacattggcg tgcgatccaa
     1081 gctagccttg cttaattttt gaactgttcc cgcggaaaat tcgccgttgc cataacaccg
     1141 ccatcaacca ccagcggatg gccatagatg aagtctgact cttcactgac cagaaacagc
     1201 gctgctttcg cctgatcctc ggcagtccct agacgaccag caagattctg aaacgtcctg
     1261 ttcccctcca catcatcgtc gtagccaacg ggtgagcccg agcggttcgg caccacgcaa
     1321 ttgacccgaa tcttgtattg cgcgagctgc accgccatgg cacgagtcat attgatgacg
     1381 ccggctttcg cggcggcgta agcaataccg tcgaaacgtc cgatcatacc cgatgtcgag
     1441 ccaaagttga cgatcttgcc gccgcgcccc tgcttgcgca tttcgtcggc gacatacttg
     1501 gtcatgagat atggaccttt gaggctgacg gcgatcacct tgtcgaattc ctcttccttg
     1561 ctgtccagga tcgtggcgtg atccgtcagg gccgcattat taacgagaat gtcgattgcg
     1621 ccaaaccgag cgactacttt tttaaccatc gcctgcacat cgtcggattt ggaaatatcg
     1681 ccgagcacca gcatcgcctc atgatcggct ctgttgagtt cgtccaccac cgcctggccg
     1741 cggccctcgt gcacttccgc aaccgcgatt ttcgcgccct ccgacgcgaa cagctttgcc
     1801 atcgctttgc cgatgtttct tccggcgccg gtgatgagcg cgacttgttc ctttaatttc
     1861 attgcagttc ctttccggtt tttcttttac tcggtaagcg gcgctttctc aataatgcac
     1921 gatgagcggc aaccgcgttg tcattctaag cattagtttg tgtaaaacca gccgctcttc
     1981 ttgcgacacg cgcaggattg ccgaacgaaa cccccaacca catggccctc gagcgagcac
     2041 gagtggccgc tgcatttggc gttatccgat aaccgattcc cgagtagcgc gaagcgcgta
     2101 tcgagggata ccgcgctctc cttactcggg cgggaactca cagctgcaga tcttccggcg
     2161 gcgtaaacgt gtagacatcg aacgtgttga gagtacaagc gagcatggca tagtaaccga
     2221 tggtcgcagt gagctcgacc atgcccttct ctcccaatcg gccgtagatc gcctgaaaag
     2281 tcgattcgct cacccggtgg ttgtgaatca tctcgcgcac gaagctgaca atctgcgcat
     2341 cttcagaaga aaacttctcc gtgccctcct tttgatacac cgcacgaatg gtttccattg
     2401 gaataccggc tttctgtcca tgcataatat gcgcggccca ctcatgttta cattccagct
     2461 cccgcgctgc caccagcgcg gtaaactcga cgactttgtg gtccagagtc gattcgaagc
     2521 ggatgtaact acccagctgc atcgtgcgct gcgccaactc cgggctgtgc agcaatgcga
     2581 gccaaggccc gcccaccacc ccgcggcttt gggcaatggc ctc
//
LOCUS       DASULZ010000505         2335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_873236, whole genome shotgun sequence.
ACCESSION   DASULZ010000505 DASULZ010000000
VERSION     DASULZ010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873236"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VFY96_10335"
     CDS             complement(<1..353)
                     /locus_tag="VFY96_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5606904.1"
                     /translation="MKAGSYVDGQWFHPKSTRLVRNLNPADPDDVIAEFPAATTDDVQ
                     RAIECAQEAAKGWRKTPGPERGRVLWRAADIARQRADEIARTLTREEGKIFKEAKGEV
                     MKGISLLEFYAGEGFR"
     gene            complement(406..1461)
                     /locus_tag="VFY96_10340"
     CDS             complement(406..1461)
                     /locus_tag="VFY96_10340"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5606905.1"
                     /translation="MKSFFSALLLFAVGLVSAVSAQTSKPTPIDQLAAYNKPDRERVL
                     YGGAKNEGKITWYTSLAGESYKQLAAAFEAKYPGVKVESYRATRQEMSARITAESQAQ
                     RYIVDTIETTIPLLKLLKENSMLVPYYFPTQAKFPDNTKEKTGKDLVFWAIDRESHIG
                     LAYNRNSIASKLAPRNYDGLLRPELKDKIAFAGSDTGVTVIGAMLKFKGEEYVKKLKS
                     QNPAIHNVSGRALLDLVISGEVGVSPTIFRNHVEVSLKAGAPIEWIPMDVVPANSGST
                     GVSAKAPHPHAALLLADFILGEGGQKVLEQYEYGNPTKDYGFKRWYPEQGLSAEQIDK
                     LEEKWRATLRDLTKRSF"
     gene            complement(1605..>2335)
                     /locus_tag="VFY96_10345"
     CDS             complement(1605..>2335)
                     /locus_tag="VFY96_10345"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5606906.1"
                     /translation="LTFIRKQTDKKVKYLISSHSAGDHASGAWHFREDKPVYIATKNQ
                     VHDLRMQQLEEFNERKNSNDPRNAGYKDRELIPPDIGFDGSMTLYFGGLTFQITAEGR
                     GHSTGDLTVYIPQKRVYLMGDLLDTEIHPGQGESAGVFYSNVKGWLQILDKIMARNLP
                     VETYVPGHGPAHIGRGLKDLEEQKRYFVVMRDEVAKMIAAGKTLDQIEKEFKIPQEFA
                     HYARPERLKQFYKLFYNQLMETGY"
BASE COUNT          471 a          625 c          641 g          598 t
ORIGIN      
        1 cgaaaaccct cgccggcata gaactccagg agtgaaatgc ctttcatgac ttcacccttg
       61 gcttctttga aaatctttcc ctcttcgcgc gtgagggtgc gcgcgatttc gtcggcgcgc
      121 tggcgcgcga tatcggcggc ccgccaaagc acgcgcccac gctccggtcc gggggttttg
      181 cgccagccct ttgccgcttc ttgggcgcat tcgatggcgc gttgcacgtc gtcggtggtc
      241 gccgcgggaa actcagcgat gacgtcatca ggatcggcgg gattcagatt gcgtaccaga
      301 cgcgtcgatt tgggatgaaa ccactgtccg tccacataag agccggcctt cataaccgta
      361 cctccgattg aatgcgcgcc aggtcgatgc gaaaattttg ctctgctaaa atgatcgctt
      421 ggtcagatcg cgcagagtcg cgcgccattt ctcctcgagc ttatcgatct gctcagcgct
      481 caacccctgc tctggatacc agcgcttgaa gccgtagtcc ttggtcggat tgccgtattc
      541 gtattgttcg agaacttttt gtccgccctc gccaaggata aagtcggcga gcaacaaggc
      601 tgcatgcggg tgtggcgctt tagctgagac gccggtagac ccggagttgg ccggcacgac
      661 atccatcgga atccattcga tcggtgcgcc ggctttgagc gaaacctcga cgtgattgcg
      721 aaagatcgtc ggtgaaacgc cgacttcgcc ggaaatcacc agatcgagca aggcacggcc
      781 ggaaacattg tgaatcgccg gattctgact tttgagtttc ttgacgtact cttctccctt
      841 gaatttgagc attgcgccga tcacggtcac accggtgtcg ctgccggcga aggcaatttt
      901 gtccttcaac tccggacgca ataagccgtc gtagtttcgc ggcgccaact tagaggcgat
      961 cgaatttctg ttataggcga ggccgatgtg cgattcgcga tcgatcgccc aaaacaccaa
     1021 atccttcccg gttttttcct tcgtgttatc cggaaatttc gcttgggtcg gaaaataata
     1081 gggcaccagc attgaatttt ctttgagaag cttcaagagc ggaatcgtgg tttcgatggt
     1141 gtcgacgatg tagcgctgcg cctgggattc tgcggtgatt ctggcactca tttcctggcg
     1201 cgtcgcccga taggattcga ccttgactcc tggatactta gcttcgaatg ccgcggcgag
     1261 ttgtttgtac gattcaccgg cgagagaggt gtaccaggta atcttgcctt cgttttttgc
     1321 gccgccgtat aagacccttt cccggtccgg cttgttgtac gcagcaagct ggtcgattgg
     1381 cgtcggtttg gaagtttgcg ccgacaccgc ggaaaccaaa cccactgcga ataatagcaa
     1441 ggcgctaaag aaagacttca ttgtctaccc tccgtgataa gtgatgtgcg aggtgtcatc
     1501 ctggccgccc ggcgaagaaa ctaggtatga atttgtgaga tgcttcgctt gctcagcacg
     1561 acacaatggt catgatactt tctacctgcg gcgactaatt ctttctagta tcccgtttcc
     1621 atgagctgat tgtaaaacag cttataaaac tgctttaggc gctcgggccg ggcgtaatgg
     1681 gcaaactcct gcggaatttt gaattccttt tcgatctggt cgagcgtctt gcccgcggcg
     1741 atcatcttcg cgacctcgtc gcgcatcacc acaaagtagc gcttttgttc ctcgaggtct
     1801 ttgaggccgc ggccgatgtg tgctggccca tgaccgggga cgtaggtctc caccggaaga
     1861 tttcgcgcca tgatcttatc gagaatctgg agccaacctt tgacgttgga atagaaaact
     1921 ccggcagatt cgccttgtcc aggatggatt tcggtgtcga gcaaatcgcc catcaaataa
     1981 actcgcttct gcgggatata gacggtgaga tcgccggtac tgtggccgcg gccttccgcc
     2041 gtaatctgaa acgtcagccc gccgaagtat aacgtcatgc tgccgtcgaa tccgatatcg
     2101 gggggaatca attctctgtc tttgtagccg gcgttgcgcg ggtcattgga atttttccgc
     2161 tcgttgaatt cttccagttg ttgcatgcgt agatcatgaa cttgattttt ggtcgcgatg
     2221 taaaccggtt tatcttcgcg aaagtgccat gctcctgaag catgatcgcc ggcgctgtgc
     2281 gacgagatca gatactttac cttcttatcg gtctgcttgc ggatgaaagt gagag
//
LOCUS       DASULZ010000506         4111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_162574, whole genome shotgun sequence.
ACCESSION   DASULZ010000506 DASULZ010000000
VERSION     DASULZ010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162574"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VFY96_10350"
     CDS             <1..535
                     /locus_tag="VFY96_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX5606907.1"
                     /translation="VDHLFRRGQLISAAAYSLGHGTNDAQKTMGIIAGALFTVPAYRY
                     LVSDASGHLAIPFWIVLLAHAAIGLGTLSGGWRIVHTMGSRITRLQPIGGFAAEAAGA
                     VTLFGAAHFGVPVSTTHTITGAIVGVGSLRRLRSVHWNVAGRIVWAWILTIPASALIA
                     MFCWLAIDIARKMFQSV"
     gene            complement(551..1171)
                     /gene="recR"
                     /locus_tag="VFY96_10355"
     CDS             complement(551..1171)
                     /gene="recR"
                     /locus_tag="VFY96_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX5606908.1"
                     /translation="MSLYPAPLARLIEELSRLPNIGEKTAARLAFHMLKGNKDDVLRL
                     ADSIGKLRQQMGLCQRCFGFSEVNLENQDGALCAVCRNPEREQDKICVVEEPADLLAV
                     EKSQEFRGLYHVLHGTISPLDGMGPEALRIRELLERLRDSAVREVIVATNPTMDGEAT
                     ALYLSKVIKPTGISVTRIARGLPMGGDLEYTDAVTLGKALEGRREI"
     gene            complement(1168..1488)
                     /locus_tag="VFY96_10360"
     CDS             complement(1168..1488)
                     /locus_tag="VFY96_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX5606909.1"
                     /translation="MASIGGMGNLLKQAQEMQARMAKIQEELANKTVQGSAGGGMVQA
                     TVNGQFNVTAIKIEPSVINVEEKEMLEDLVLAAVNDGVRKAREMATSEMSKLTGGLKI
                     PGMP"
     gene            complement(1507..3219)
                     /gene="dnaX"
                     /locus_tag="VFY96_10365"
     CDS             complement(1507..3219)
                     /gene="dnaX"
                     /locus_tag="VFY96_10365"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX5606910.1"
                     /translation="MSYLVIARKWRPQSFADIVGQEHITRTLQNAIRTKRIAHAFLFT
                     GVRGVGKTTAARILAKALNCENGPTPTPCNECTHCEEIAQGRSIDVLEIDGASNRGID
                     EIRQIIENVRYQPAKCRFKIYIIDEVHQVTKDAFNALLKTLEEPPPSVKFILATTEPH
                     RLPATILSRCQRYDFRRIKLREIVQRLSAIVKAEAVEITDGALVLLAREADGSMRDAQ
                     SLLEQVLAFGESSDKTDARIRIDESSLHEVLGLAERQVLYQMSAAVLQGDAKQCLDLI
                     TRVFNQGSDLGRLARDLVEHFRNLLVARLIGSSSKELTAGGAQLLDLPDQEIKDLMEQ
                     AGSVSSETLLDYFDSMAASEEQIARSTTPRFVLETALVHLANLPKTIAVAELIGRLES
                     LEQKIAGKSGAAAMHERRASTPSADGNPPLKPQTIGAGAETAALKGANEKPREDHNGS
                     NLWEEFVAFVGSEKRFLSPYLESAIAVGLPPDPLKISIGERYHLAFWQDEDNLAALKE
                     LAKRFFSADVSLQIFGAPSDDSSTAASATNVSTKPDEERSPMVKEALRIFGGSVRNVR
                     RENG"
     gene            complement(3294..3392)
                     /gene="ffs"
                     /locus_tag="VFY96_10370"
     ncRNA           complement(3294..3392)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFY96_10370"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3430..3521)
                     /locus_tag="VFY96_10375"
     tRNA            complement(3430..3521)
                     /locus_tag="VFY96_10375"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3485..3487),aa:Ser,seq:gga)
BASE COUNT          819 a         1227 c         1114 g          951 t
ORIGIN      
        1 cgtcgatcat ctatttcgcc gtgggcagct gatctccgct gccgcataca gtcttgggca
       61 cgggaccaac gacgcacaaa aaaccatggg catcatcgct ggcgctcttt ttaccgtgcc
      121 cgcttaccgt tatctcgtga gcgatgccag cggccatcta gcgatcccgt tttggatcgt
      181 gctactcgcc catgccgcca tcggactcgg cacgctctcg ggcggttggc gtattgttca
      241 tactatggga tcgaggatca cgcgtctgca gccgatcggt ggatttgccg cggaagccgc
      301 cggcgccgtg acgctgttcg gcgccgcaca tttcggcgtg ccggtgtcca ccactcacac
      361 catcaccggc gcgattgtcg gtgtgggttc tctcagacgg ctgcgctcgg tgcactggaa
      421 tgtcgctggg aggatcgtgt gggcctggat cctaacgatt cccgcctccg cgctgatcgc
      481 gatgttttgt tggctggcga tcgatattgc gaggaagatg tttcaatcgg tttagctgca
      541 acggtataaa ctagatctcg cggcggcctt ccaaagcttt gcccaatgtc accgcgtcgg
      601 tgtactcgag atcgccgccc atgggcagcc cgcgggcgat tcgcgtgacc gaaatacccg
      661 tcggcttgat caccttcgac agatagagcg cggtggcttc gccatccatg gtgggattgg
      721 tcgcgacaat cacctcgcgc accgcgctat ctctcaggcg ctcgagcaat tctctgatgc
      781 gcagcgcttc cggccccatg ccgtcgagag gcgaaatggt tccatgcagg acgtgataga
      841 gtccgcggaa ttcttgggat ttttccacgg cgagcagatc cgccggttct tcgacaacgc
      901 agatcttgtc ttgttcacgt tcggggttgc gacacacggc acacagcgcg ccgtcttggt
      961 tttccaagtt gacctcgctg aaaccgaaac agcgttggca caagcccatc tgctgacgca
     1021 atttgccgat gctgtcagca aggcgcagga cgtcgtcctt gtttcctttt aacatgtgaa
     1081 aagccaagcg cgccgcggtt ttctccccga tgttggggag cctcgacaat tcctcaatta
     1141 gacgggcgag aggcgcggga tacagactca tggcatacct ggaattttca acccgccggt
     1201 cagcttgctc atttccgaag tcgccatttc gcgtgccttg cgcacgccat cattgacagc
     1261 ggcgaggacc aaatcctcga gcatttcctt ttcctcgaca ttgatcactg acggctcgat
     1321 cttgatcgcg gtgacattga attggccgtt caccgtcgcc tgcaccatgc caccgcccgc
     1381 cgatccttgt acagttttat tggcgagctc ctcctggatt ttggccatgc gcgcctgcat
     1441 ctcttgcgcc tgctttaaga gattacccat gccgcctatg cttgccatta ttttcctcct
     1501 ggacgattag ccgttttccc gtcgcacgtt gcgcaccgat ccgccaaaaa tgcgcagcgc
     1561 ctctttgacc atgggactgc gctcttcatc cggcttcgtg gaaacgttcg tcgcgctagc
     1621 agcagtgctg gagtcatcgg agggcgcgcc aaaaatttgt aaactcacat cggcagaaaa
     1681 aaatcgtttc gccagctctt tcagcgccgc caaattgtcc tcgtcctgcc agaacgccag
     1741 gtgatagcgc tctccgatgc tgatcttcag tggatcgggc ggaagtccca ccgctatggc
     1801 cgattccaga tagggcgaaa gaaatcgctt ttctgatccg acaaacgcga caaattcctc
     1861 ccacaggttc gatccattgt gatcctctcg aggcttctca ttggcgccct ttagcgcggc
     1921 tgtttcagcg ccggcgccaa tcgtctgagg tttgagcggc ggattgccat cggcggacgg
     1981 cgtgctcgcc cgccgctcgt gcatggccgc cgcgccggat ttcccggcaa tcttctgttc
     2041 caacgattcg agacgcccga tcagttcggc cacagcgatc gttttcggca gattcgccag
     2101 atgcaccagc gccgtctcca agacaaacct cggcgttgtc gagcgcgcga tctgttcttc
     2161 actggcagcc atagagtcga aataatcgag cagcgtttca ctcgagaccg atccggcctg
     2221 ctccatcaaa tccttgatct cctggtccgg cagatcgaga agttgagccc ccccagccgt
     2281 aagctctttg cttgagctgc cgatcagtct cgcgacgagc agatttctaa aatgttccac
     2341 gagatcccgc gccagtcttc ccagatcaga tccctgatta aaaacgcgcg ttatcaagtc
     2401 tagacactgt ttcgcgtcgc cctgcagcac cgccgccgac atctgataaa gcacttgacg
     2461 ctctgccaac ccgagcactt catgcaatga actttcgtcg atgcgaatgc gtgcatcagt
     2521 cttgtccgat gactcgccaa acgccagcac ttgctccaaa agcgactgcg cgtcgcgcat
     2581 gctcccatct gcttcgcgcg ccagcagcac cagagcgccg tcggtgatct cgactgcctc
     2641 cgctttgacg atcgcgctca gccgttggac gatctcacgc agtttgatgc ggcgaaaatc
     2701 gtaccgttgg cagcgcgaga gaattgtggc cgggagccgg tgcggctcag tcgtagcaag
     2761 aataaatttt accgaaggcg gcggctcttc gagcgttttc aacaaggcat tgaaggcatc
     2821 cttggtgact tgatgaactt catcgatgat atagattttg aagcggcact tggccggctg
     2881 gtagcggacg ttttctatga tttggcgaat ctcatcgatg ccgcgattcg aagcgccgtc
     2941 gatttcgagg acgtcgatag acctgccctg ggcgatttct tcacagtggg tgcactcatt
     3001 acagggagtg ggcgtcggcc cgttctcaca attcaatgct ttggccaaaa tccgcgccgc
     3061 ggtggtcttg cctacccccc gcactccggt gaacaaaaag gcatgcgcga tgcgttttgt
     3121 tcgaatggcg ttttgcagcg tccgggtgat gtgctcctgc cccacgatat cggcaaacga
     3181 ctgagggcgc cattttcgcg caatcactag gtatgacata gctcaaatag ccgctaaacg
     3241 acccgactaa tgatcaatgg attgtctatg cgcgctgcgg ggtgtccatt gctgacagcc
     3301 aggttacccc gtggcacagg ggctgatccg ctaccgctgc ttccttccgg acctgacggg
     3361 ttttacggtg ccgccttgca cgggacctaa ctatcagcaa tcgacatcca gcttcgctca
     3421 atgttgtttt ggcggagagg gagggattcg aaccctcggt acggggttaa ccgcacacac
     3481 gatttccagt cgtgcacctt caaccgctcg gtcacctctc cgttctggga tttcaactac
     3541 ttggatgcac tgtctgctcg caccactcgc caaatgtctc catcgggatt tgtggggctt
     3601 tggtaaccgt cctgccaagc taccacacga aattatcagt ggcaaagacg aaacgctatc
     3661 acgcgcaagt tatatgattg agatgttgga gtcgcaaacc gaagcggcgg gcgcatgatt
     3721 gaagctacgt tcgccgactt cgccaaggtc gcaaaacggc acggcctcac acccgagtta
     3781 ttggtccaac gattcaagac caagatcgat agagcatcgg agttctgcca tcggatcttt
     3841 gaacgacagt atgcggcggc ggcattgacg ctgtcagcgt gcagcagctc gccattgtgg
     3901 accttgccgg caagcaaaag ctcctgcact gagccaccga gcccgtcaac tatacttact
     3961 aagtgagcat cctgagcgat gtgcgaatcc gttctgtaat gacggagcga ggagatcgcg
     4021 caagcacggc cgctattgct cggatcgctg ccgaatggac ggctacgtga ttaggcgcgc
     4081 cagaaagacg attagtaaac ttggaatcgt c
//
LOCUS       DASULZ010000507         8963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_873358, whole genome shotgun sequence.
ACCESSION   DASULZ010000507 DASULZ010000000
VERSION     DASULZ010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8963
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873358"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VFY96_10380"
     CDS             <1..471
                     /locus_tag="VFY96_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5606911.1"
                     /translation="IVLALVVVAVLGSGIWNVILAISIPMIPRAALVVRSSALALRQM
                     AFIEAALTLGSSHWRVIFWHMVPNVMAPYLIMLTAFLGQAILLEASLSFLGLGVAEPI
                     AAWGLMLRGAAVEFAERAPWMALFPGLAISLAVFAFNTFGDSLRDALDPKLRLL"
     gene            complement(486..1088)
                     /locus_tag="VFY96_10385"
     CDS             complement(486..1088)
                     /locus_tag="VFY96_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606912.1"
                     /translation="MPDDLFKLKSNDSGGYFAMALRAIGQDLAELFPQQIELYYQNET
                     FDVRVRCDRKRAEKKTPAAAKSGLKNVIHKLATYRLDKAADGTDIAVFEHHYNSEEIG
                     RLDRGGIHRRSQAGKVPDINNLGEALRTIGRIVDAQGGQLVRILKDPRRIVFDYKDKQ
                     GTSHNKEMTRSELFKVQQSYYEKRSGSGGVDPWKGQDWRR"
     gene            complement(1101..1682)
                     /locus_tag="VFY96_10390"
     CDS             complement(1101..1682)
                     /locus_tag="VFY96_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606913.1"
                     /translation="MNLSELLKLMVHQTSGFHARALRVIGQDLARLVPENIAIELHGD
                     SFVAHGLCAKNRMEDKDSPRTLCAVKKFFTRMAGIIRAHSHEPDLEFVPFTRTYKISD
                     LQHLDESATGHRGNPEGMPDIYTLGERLRTIGKVIDGHNGRVIRIFKDLHQIVFEYQD
                     GNGKSRKEELNNTQLYQLQQSYTAGRGTIESGN"
     gene            1968..3053
                     /locus_tag="VFY96_10395"
     CDS             1968..3053
                     /locus_tag="VFY96_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia lyase-activating protein"
                     /protein_id="HEX5606914.1"
                     /translation="MVGANLKRAGNELGAKTTYQLFLEKEAVPAVRGFHVEDINAVEL
                     FPWARVGGRGVYLNLEGSEGVNDCYICEIAPGASLERQKHLLECLVYVVSGRGATTIW
                     QDGGKRQTFEWGEGALFSPPLNAWYQHFNGQGDKPVRLLAMTNAPTVLNLYHNIDFVF
                     NCDYRFIDRYAGEEDFFSGRAKIPGEGFHDTNFIRDVRSYELPERSDRGAGGKMLMIE
                     MSNNVMSAHISQFPVGTYKKAHRHGAGAHVIILRGEGFSVMWREGEAIKRYNWRAGSL
                     LVPPERWFHQHFNIGGEPARYLALKPFSSRKFPGLRKQWGTSESIKTGGDQIEYEDED
                     PRIRTMFEEELAKRGVESRMESVFKAA"
     gene            complement(3229..3663)
                     /locus_tag="VFY96_10400"
     CDS             complement(3229..3663)
                     /locus_tag="VFY96_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HEX5606915.1"
                     /translation="MAEDGKAIKKPLPEITPVNQAFWDGAKAGKLMMQKCRDCGTWVF
                     CPRPVCGECASDKLEWVQVSGKGKIFSFTVIREVVGQALRGFAPEIPYVTAWVDLDEG
                     PRFCSNVIGCPIDKVYIDMPVEVVFEDTGEGITLPKFKPAAA"
     gene            complement(3772..4965)
                     /locus_tag="VFY96_10405"
     CDS             complement(3772..4965)
                     /locus_tag="VFY96_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX5606916.1"
                     /translation="MSIRGKTAVLGIGETPTDRLGSKPGEPKKSTAEYLAWAARLAME
                     DAGLTKKDFDHQGLAAIYTTNHSQPFWPEEVAAILGITPGVSLAGGNGGASSVSLLGH
                     AAAAISAGLCDLILVIAAAAPFSERGGHRGTPADTRDYEMPFGVMGPNCKISFVMSRY
                     MHDSGMTEEHFGKIAVTGRYHASLNPNAYLRKPITIEDYKKSRLISDPIRLFDCVMPA
                     NGGKAYIMTSAERAKSMRKPPVYLLGWGECNNASSGPRYRANPLITGITQAGKRAFEM
                     AGVTHKDIRCLNLYDDYIPIVMIQIEDLGFCGRNDKAFFERTDFTFKGDLPIQTSGGM
                     INCGQPSTTGGMLHVLETVRQIRGEGGDRQVPNIKFGISTGVGAVNYGKNFGCTAAAI
                     LGSEV"
     gene            5369..6370
                     /locus_tag="VFY96_10410"
     CDS             5369..6370
                     /locus_tag="VFY96_10410"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606917.1"
                     /translation="MKKNIAVLILCAMLFALGSSADAQQPAKIPRIGVLSTAGDSRIP
                     SFQIEAFRKGLLDIGYIDGKNILVEYRYAEGNQERLPAIVAELVQLKVDVLVVTALTA
                     IRIAKQATQTIPIVMVILSDPVATGIVDSLARPGGNITGFTRLSRELSGKRLELLKET
                     VPKISRAAVLWDGNAPGAAAALKEYQTASHALKMRIESLEVRGPDPDLETTMLVAARP
                     RFGALVTINNALVRRYSKRIADLAIKKKLPSMYEGRDYIEAGGLMSYSANDTEAYSRA
                     AIYVDKILKGKKPADLPIEQPTKFEFVINLKTAKQIGLSIPPNVLARADRVIKERRQ"
     gene            6727..8229
                     /gene="ltrA"
                     /locus_tag="VFY96_10415"
     CDS             6727..8229
                     /gene="ltrA"
                     /locus_tag="VFY96_10415"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEX5606918.1"
                     /translation="MHVGGESHSFIVPKRPANKGRVPRPAELVEGRELTKENTEQLLL
                     DRTQSRKPNGRQMVARSRGLLGVREAARRERKQKFTSLLHHLTPELLRVSFFELKKQA
                     APGIDGERWQEYAVDFERRIDDLHGRIHRGAYRAKPSKRSYIPKPEGGMRPLGIAALE
                     DKIVQQAARTVLECIYEQDFLGFSYGYRPRRSSHQALDALFVGISRRKVDWIIDADIR
                     GFFDNISHEWLMKFVEHRIADKRMLRLMKKWLRAGVSEDGEWSPTKVGTPQGAVISPL
                     FANVFLHYVLDLWINDWRKRQAKGEVIIVRYADDFVIGFRKQSDARKCLAALGERLAK
                     FGLELHPEKTRLIEFGRYAEARRAKRGDGPPETFEFLGFTHISGKTLGGEFTIHRRTS
                     RKKFQAKLAELKERLSRRRHDDLAKVGEWLQSVYRGWCQYYAVPGNCARLQQFRAAIQ
                     VMWLRNLQRRSQRGRHLTWAKFSKLCKRWLPTPKILHPYPSVRFACQHPR"
     gene            8305..>8963
                     /locus_tag="VFY96_10420"
     CDS             8305..>8963
                     /locus_tag="VFY96_10420"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606919.1"
                     /translation="MRTIGGKNFLGLTVAVVLVTLCSSAQAQQPTKIARVGYVDAGSR
                     ATTGHRATAFIQGLRDLGYVERKNILIEYRWAEGKLESLPSLVNDLVRLRVDVIISSA
                     TPAIQLAKEQTTTIPIVMAGVTDPVGVGFVSSLARPGGNVTGLTHLSPDLTGKRLEIF
                     KEVVPQLVRVAVLWNPNQPGQPAAFKAAQVSARTLKLTLISMETRTREEIIRVLSTVE
                     K"
BASE COUNT         2028 a         2339 c         2594 g         2002 t
ORIGIN      
        1 atcgtattgg cgctcgtggt ggttgcggtt ttaggatccg gaatatggaa cgtgattctt
       61 gcgatctcga ttccgatgat tccgcgggcc gcgcttgtcg tgcgcagtag cgctctcgcg
      121 ctgaggcaga tggcgtttat cgaagctgca ctgacgctcg gatcgagcca ttggcgggtg
      181 atcttttggc acatggtgcc caacgtgatg gcgccgtact tgattatgct caccgcgttt
      241 ctggggcagg cgattctatt ggaagcttct ctgtcgtttc tcggtttggg cgtggcggag
      301 ccgattgcgg catggggtct gatgctgcgc ggtgcggcgg tcgagttcgc cgagcgcgcg
      361 ccgtggatgg ctttgttccc cggactagcg atcagcctgg cggtgttcgc attcaatact
      421 ttcggggatt cactgcggga cgcgctcgat cccaaattgc ggctactgta aattcgacgg
      481 taggactagc gacgccaatc ctggcctttc caaggatcaa cgcctcccga gccgctgcgc
      541 ttttcataat aactttgttg caccttgaac aactcggatc gcgtcatttc tttattgtgc
      601 gacgtgccct gcttgtcctt atagtcgaag acaatgcggc gcgggtcctt gagtattcgg
      661 actagctgtc cgccttgagc atcgacgatt cggccgatgg ttcgaagcgc ttcgcccaag
      721 ttattgatgt cgggaacttt gcctgcttgg ctccggcgat ggatacctcc ccgatccaat
      781 cggccgattt cctccgagtt gtagtgatgt tcgaagacgg cgatatcggt gccgtctgcg
      841 gctttgtcga gccgataagt ggcgagttta tgaattacat tcttcagacc ggatttcgcc
      901 gcggccgggg ttttcttttc tgcccgtttg cggtcacaac gaacgcggac gtcaaacgtt
      961 tcgttttggt agtagagttc gatctgctgc gggaacagct cggcaagatc ctgtccaatg
     1021 gcgcgcaatg ccatggcaaa atagccaccc gagtcgttgg atttcagttt aaaaagatcg
     1081 tcaggcacgg ggtgcgatgc tcaattccca gattcgatgg tgccgcgtcc ggcggtgtaa
     1141 ctctgctgca gctggtacaa ttgtgtattg ttcagttcct ccttgcgcga cttgccattg
     1201 ccgtcctggt actcaaagac aatctgatgc aaatctttga aaatgcgaat aactctgccg
     1261 ttgtgcccgt cgataacttt tccgatggtt cgcagccgtt caccaagggt gtaaatatcg
     1321 ggcatgcctt caggattgcc tcggtgcccc gtcgcgctct cgtcaagatg ctggagatca
     1381 ctgatcttgt aagttcgagt gaacgggaca aattccaaat caggctcgtg tgagtgcgcc
     1441 cggatgatgc ccgccatccg cgtgaaaaat tttttcaccg cgcacaaagt ccgcggcgag
     1501 tctttgtcct ccatacggtt ctttgcacaa aggccgtgag cgacgaaact atcgccgtgc
     1561 aattcgatgg caatattttc cggcactaat cgagcaaggt cctggccaat aactcgcaaa
     1621 gcacgggcgt gaaatcctga ggtttggtgc accataagct tgagcaactc actaagattc
     1681 acggctcaca ggggattgat acagcaggtg acggccgatg cgggacgtga gttgacgagt
     1741 tgattgcgct gtcaccgcgc ttgatccatt ttccagatca cgtcaggttt tgcactgcgg
     1801 cttcatgcac aggctggggc tcaattgcgc aatagggtac tccaaaacca aaaaacgtca
     1861 acaaaaatct tcgtctggca gtgataactt gaccgtgtgt gagcggacgt ataagaacaa
     1921 agacgacgaa ttcagcggtc attggcgctt ttgggaaagg acgcaagatg gttggggcaa
     1981 atctcaagag agcgggcaac gagctcgggg caaaaactac ctaccagctc tttctcgaaa
     2041 aagaagcggt tccggcggtg cgcggatttc atgtggaaga tatcaacgcg gtcgaacttt
     2101 tcccatgggc gcgagtcggc ggccgagggg tttatctcaa tctcgagggt tccgagggcg
     2161 tgaacgattg ctacatttgc gaaattgcgc ccggcgcgtc gcttgagcgg caaaagcact
     2221 tattggagtg tctggtttat gtcgtcagcg gccgcggcgc gacgacgatt tggcaggacg
     2281 gcgggaagcg acaaaccttt gaatggggcg aaggtgcgct tttttcgccg cctttgaacg
     2341 cctggtatca acatttcaac ggtcagggcg acaaaccggt acggttattg gcgatgacca
     2401 atgcgccaac cgtgctgaat ctctatcaca atatcgattt cgtttttaat tgtgactacc
     2461 gttttatcga ccgttatgcg ggtgaggagg attttttcag cggccgagcc aagattcccg
     2521 gagaaggatt tcacgatacc aattttatcc gcgacgtgcg cagctatgag ctgccggaaa
     2581 gaagcgatcg cggcgctggc ggcaagatgt tgatgatcga aatgtcgaat aacgtgatgt
     2641 cggcgcatat ttcacagttt cccgtcggaa cgtataagaa agcccatcgg cacggcgccg
     2701 gcgcacatgt gatcattctc agaggcgagg gtttttcggt catgtggcgt gagggcgaag
     2761 cgatcaagcg ctacaactgg cgtgcaggaa gcctgctcgt accgccagag cggtggtttc
     2821 atcagcactt caacatcggc ggcgaaccgg cgcgttatct agcgctgaaa ccgttcagca
     2881 gccgaaaatt tcccggactg cgtaagcagt ggggcacctc cgagagcatc aagacgggtg
     2941 gcgatcaaat cgaatacgag gacgaagacc cgcggattcg caccatgttc gaggaagagc
     3001 tggcaaagcg cggggtcgaa agccgcatgg agagtgtgtt caaagcggca tgatcgcgaa
     3061 gcagactcga ggctcttggt ccgtcattcc cgcatgtatt cagcgggaat ccattcgcct
     3121 cttatctcgg ggcatcaacc tggatgcccg cctgcgcggg catgacaatt ttagttagcg
     3181 aggagtttgg taccaacgcc gctccatcgg atacggtggt attttcaact acgccgctgc
     3241 gggtttgaat ttcggcaatg tgataccttc acccgtatct tcgaaaacca cttccaccgg
     3301 catgtcgata tagactttgt caattggaca gccgatcacg ttactgcaaa agcgcgggcc
     3361 ttcatcgaga tcgacccaag cggtgacgta cggaatctca ggagcgaagc ctctgagcgc
     3421 ttggccgaca acttcgcgga tcacggtgaa ggaaaaaatt ttccctttcc cgctcacctg
     3481 aacccattca agcttgtcac tggcgcattc gccgcaaacc ggccgtggac aaaataccca
     3541 ggtgccgcaa tcacggcatt tctgcatcat gagcttgccg gctttggcgc cgtcccagaa
     3601 cgcttggttt acgggcgtga tttccggaag cggtttcttg attgctttgc catcttctgc
     3661 catctgaaac tcctaaataa tttaagctcg cacgcgagca acaaagctta attttcacca
     3721 cgaagcgcga agcacacgaa ggttggtgag ctctcggtct cagttcctcc gttacacttc
     3781 gctgcccaga atcgccgcgg cagtgcagcc gaaatttttg ccgtagttca ctgcgccaac
     3841 gccggttgaa atgccgaatt ttatgttcgg tacttggcga tcgccgcctt cgccgcgaat
     3901 ctggcgtacg gtttcgagca cgtgcagcat gccaccggtg gtcgaaggtt ggccgcaatt
     3961 gatcatgccg ccgctggttt ggatcggcag atcccctttg aaggtaaaat cggtgcgctc
     4021 gaaaaacgct ttgtcgtttc ggccgcaaaa gccgagatct tcgatctgga tcataacgat
     4081 cggaatatag tcgtcgtaaa gattgagaca gcgaatatct ttgtgcgtta cgcctgccat
     4141 ctcgaaggcg cgcttaccgg cttgggtgat tccggtaatc aatggattgg cgcggtagcg
     4201 cggcccgctg ctcgcgttgt tacactcgcc ccaaccgagc aaatagaccg gcggtttgcg
     4261 catcgacttg gcgcgctcgg cggatgtcat gatgtaggct ttgccgccgt tggccggcat
     4321 cacgcaatcg aatagccgaa tcgggtcaga gatcaaccgc gattttttat aatcctcgat
     4381 cgtgatcggt tttctaagat aggcgttggg attgagcgag gcatggtatc gtcccgtgac
     4441 cgcgattttc ccgaagtgtt cctcggtcat gccggagtcg tgcatgtagc ggctcatgac
     4501 aaaggaaatt ttgcagttag ggcccatcac gccaaacggc atttcgtagt ctcgggtgtc
     4561 tgcgggagtg ccgcgatgac caccgcgctc actgaagggc gccgccgccg cgatcaccaa
     4621 tatcagatcg caaagacccg cactgatcgc cgccgctgcg tgaccgagca aagataccga
     4681 gctcgcgccg ccattgccgc ccgcgagtga aacgccgggc gtgatgccga ggatcgcagc
     4741 gacctcttcg ggccaaaacg gttgcgaatg attagttgtg taaatcgccg ccagtccctg
     4801 atgatcgaaa tcttttttcg ttaagccggc gtcttccatc gcgagtcggg cggcccaggc
     4861 gagatactca gcggtggact tttttggctc gccgggtttg ctgccaagcc ggtccgtcgg
     4921 cgtctcgcca atcccgagga cggctgtttt tcctcgtatg ctcataagaa atttcttctc
     4981 cttattcatc gagcaatatt tcagcgctct cggcgtgtca aggtagctga cggcaacgaa
     5041 cctcaccgtc aatctcaatt cttcgctgcc cctgtttaat cgttagggct aaagccgatg
     5101 aatcaatatg gatacaccca gtatcaagtc cgcgcgcgcg caggcatgga tgcaagagtc
     5161 gttaggcaga acgaccacag catttttcag gctaatcgat gattcggccg tggcaccgtt
     5221 cgtgcttcgc ataactgcgc cttttgaacg tgtcacagcg tttattcgtt gttttcatgt
     5281 tggtcgattc tcccaacatt ctgatgagac gaagcaggcg gccaagtaag aacgggggag
     5341 tgctgacggg atcgcggcag aggcggacat gaagaaaaac atcgcggtcc taattctctg
     5401 cgccatgctt tttgctcttg gctcgtccgc cgacgcgcaa cagccggcaa agattccgcg
     5461 cataggagtt ttgtccaccg ctggcgattc ccgcattccg agctttcaaa tcgaagcgtt
     5521 ccgcaaagga ttactggaca tcggttatat cgatgggaag aacatcctgg ttgagtatcg
     5581 ctatgccgaa gggaatcagg agcgtcttcc agccatcgtg gcagaactgg tgcaactcaa
     5641 agtcgacgtt cttgtcgtca ccgcattaac tgccatccgt atcgccaagc aggcaaccca
     5701 gacaatcccc atcgtcatgg tgattctttc agatccggtg gcgactggga tagtggacag
     5761 tctggcgcgg ccgggcggga atatcaccgg atttaccaga ctcagccgcg aactgagtgg
     5821 aaaacgtctg gaattgctga aagagacggt tccaaaaata tcgcgtgccg cagtgctttg
     5881 ggatggaaac gcacccggag cggctgctgc tttgaaagag tatcagactg cctcgcatgc
     5941 gctgaagatg cgcattgaat cgttggaggt aagggggccc gatcctgatt tggagacgac
     6001 aatgctcgtc gccgcccgcc ccagatttgg cgctctcgtc accatcaata acgcattagt
     6061 tcgacgttac tcgaagcgaa ttgccgacct cgccatcaag aagaaactac cgtcaatgta
     6121 cgaagggaga gactatatag aggccggcgg cctgatgtcc tattcagcca acgacactga
     6181 ggcctacagt cgtgcggcga tttatgtaga caagatcctg aaaggaaaga agcctgcgga
     6241 cttaccgatc gagcaaccga ccaagttcga attcgtgatt aatctgaaaa cggcgaaaca
     6301 gatcggtttg agcattccac cgaatgtgtt ggcgagagcg gaccgggtga tcaaggagcg
     6361 tcggcaataa gccggaggga ggtagcgaat gaaagagtcg tacagggtaa gacctagcca
     6421 gtcgccttgg cctcgagtga tgcgtagtgt cgggtaaccg gcaggacgaa gcgttcacag
     6481 aggtacaatc gggcggtgta tagagctccg aaaacaccct tgtgaggtgg ccgaccttat
     6541 cttgatatgg ggaaggcaat agtcatgtcg aacgatcacg gcgagatcga tgtggccccc
     6601 acggagtcaa agaccacagg cacgattgga aacctttctc acggaagccg agagaccccg
     6661 ctaacatccg ttttgttttc catggaagcg gatcggtcgg agaaggcgcg aggccacaag
     6721 tccgacatgc acgttggcgg ggagtcacac agtttcatag taccgaagag accagcgaac
     6781 aagggtcggg tgccaaggcc tgcggagttg gtggagggaa gggaactgac caaggaaaac
     6841 accgagcagt tgctactgga ccggacacag agccggaaac cgaacgggag acagatggta
     6901 gccaggtcgc gcggattgct gggtgtacgt gaagcggcac gcagggagag gaagcagaag
     6961 ttcaccagct tgctgcacca cctcacgccg gagcttttgc gagtgagctt tttcgaattg
     7021 aagaagcaag ccgcgcccgg aatcgatggg gagaggtggc aagagtatgc agtggatttt
     7081 gagcggcgga tcgatgatct gcacgggcga atccatcgcg gggcataccg agccaaacct
     7141 tccaagcgga gctacattcc caagcctgag ggtgggatgc gcccactggg aattgcagcc
     7201 ctagaggaca agatcgttca acaggcagcg cgcacggtac tggaatgtat ctatgaacaa
     7261 gactttctgg ggttcagtta cggctatcgt cccagacgta gcagtcacca agccctggac
     7321 gccctgtttg tgggtattag ccggcgcaag gtggactgga tcatcgatgc ggacattcga
     7381 ggcttctttg acaacatcag tcacgagtgg ctgatgaagt ttgtggagca tcggatcgcc
     7441 gacaaacgga tgctgcggct gatgaagaaa tggcttcggg ccggagtatc agaggacggc
     7501 gagtggtccc ccacgaaagt gggaacgccg cagggggcgg tcatttcacc gctgtttgcc
     7561 aatgtgttct tacactacgt gttggacctc tggattaatg actggcggaa acgccaggcc
     7621 aaaggtgagg tcatcatcgt gcgctacgcc gacgactttg tgattggatt tcgcaagcaa
     7681 tcggacgccc gaaaatgttt agccgcctta ggcgagcggt tggccaagtt tggtttggaa
     7741 ctgcacccag agaaaacacg gctgatcgaa ttcggacgct acgcagaagc aaggcgagcc
     7801 aaacgcggag atggcccacc ggagacattc gagtttctgg ggttcacgca catcagcgga
     7861 aagaccctag gaggcgaatt caccatccac cgccgaacgt cgcgcaagaa attccaagcc
     7921 aaactcgcag agctcaaaga gaggttatcg cggcgacgcc acgacgatct ggcgaaggtt
     7981 ggcgagtggc tgcaaagcgt gtatcgcggc tggtgtcagt attatgcggt gcctggcaat
     8041 tgcgcacgcc tgcagcagtt ccgcgctgcg atccaagtca tgtggttgcg caacctccag
     8101 cgacgcagcc aacgaggccg ccacctgact tgggcaaagt tctccaagct gtgcaaacgc
     8161 tggctaccga ctccgaagat ccttcacccc tatcccagcg tgaggtttgc atgtcagcat
     8221 ccaaggtaag aaccgtatgc ggtagctccg cacgtacgga tctgtgcggg gggcggtcag
     8281 aaagaccgtc cctaccgcga ttaagtgcgc acgatcggcg gcaaaaactt tcttggtttg
     8341 accgtcgccg ttgtgctcgt gacgctctgc tcgtcagccc aggcacaaca gccgacaaaa
     8401 attgcacgag ttggctacgt cgatgccggg tcacgtgcta cgacagggca tcgtgccaca
     8461 gcatttatac aagggctgcg tgatctcggg tatgtggagc gaaagaacat cctaatcgaa
     8521 tacagatggg ctgagggaaa attagaaagc ctgccgtccc tggtaaatga tttagttcgg
     8581 ctcagagtgg acgtaataat ttcctccgcc acaccggcga tccaattagc caaagaacaa
     8641 accacgacga ttccgattgt aatggcaggc gttaccgatc cagtcggtgt aggatttgtg
     8701 agtagcctcg cccgacccgg aggaaatgta acgggtctga cacatctttc gcctgatctg
     8761 accggaaagc gcctagagat tttcaaagaa gttgtaccgc agcttgtgcg tgtcgccgtt
     8821 ctctggaatc cgaaccaacc cggtcagcca gcggcgttta aggccgcgca ggtctcggca
     8881 cgaacattaa aactgacgct catttcaatg gaaactcgaa cccgagaaga gatcatcaga
     8941 gtgttgtcca ccgtcgagaa gga
//
LOCUS       DASULZ010000508         3222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_264080, whole genome shotgun sequence.
ACCESSION   DASULZ010000508 DASULZ010000000
VERSION     DASULZ010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264080"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VFY96_10425"
     CDS             complement(<1..641)
                     /locus_tag="VFY96_10425"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5606920.1"
                     /translation="MTRSLHSVPGADFRLEKAGRHTIGLIELNNRRALNALTLGMFQA
                     IEEQLLAWEAQSDVACVIFHADSDKAFCAGGDVKTLVSELRTTGGLECAAEFFAVEYF
                     VDYLIHRYSKPVLCWADGITMGGGIGLMNGACSRVVTERTIMAMPEMSIGLYPDVGGT
                     YFLNRLPEGLGLFLGLTSVRFNAKDAVAIGMADLEIRSDKKAMVLGRLKDLPWT"
     gene            856..1056
                     /locus_tag="VFY96_10430"
     CDS             856..1056
                     /locus_tag="VFY96_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606921.1"
                     /translation="MESTINESSYSIQYDKETYNAAVCHRCGARMFPAELLEAHMDRH
                     ELKDMYLQGELKKLQFAMNRMR"
     gene            1343..2380
                     /locus_tag="VFY96_10435"
     CDS             1343..2380
                     /locus_tag="VFY96_10435"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5606922.1"
                     /translation="MKAVRMHGHGGVDQLCFENADEPRCDGAAQAIVKLSAAALNHID
                     IWNRLGVTGVRIAMPHILGADGAGVVVDVSQGVEHVRVGDRVCLYPPTGCGQCEFCVT
                     NRDFMCFRLRVLGERLEGTYAQYVKLPAQNCFPIPTGYSFEEAAAFPLVFITLWRMLI
                     TNARLEPGETILILGIGGGVASAALQVAKKIGARVIVTSGSNEKLERARDLGADYAID
                     HRSKDFAQEAQTVTDGRGVDVVLDSIGGEFWRKSLASLARGGRLVTCGATAGGQPIDD
                     IAAICAKELKIYGSTLGSREDFSQVINFLNTAKIKPVIDTIFPLENTAGAQLHLENGR
                     QFGKIVLRIPD"
     gene            complement(2494..>3222)
                     /locus_tag="VFY96_10440"
     CDS             complement(2494..>3222)
                     /locus_tag="VFY96_10440"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5606923.1"
                     /translation="LAALRHPVVLAYQLASLDWITGGRIDFGVGYGRPNDPAGEHEFE
                     ILGLDPAARIKMSEEVIQIMRRLWREDHVSHAGRFTKFDDVSIQPKPVQAGGVPIWIA
                     SNNVEPGLKRVARLGDAWINNIKSPALFAECRQKIHAYANDLGRDPDGIRAGIYFTLA
                     AGGKEAVAEGQAFLAKYYNRSYEAVANAMLCVTGSWDEVIDWMEKYIESGARTVVLRL
                     AANDQRRALEACAEALNRRSLLQR"
BASE COUNT          715 a          915 c          868 g          724 t
ORIGIN      
        1 gtccagggca aatctttcaa tctccccagt accattgcct ttttatcgga ccggatctcg
       61 aggtctgcca tgccgatcgc gaccgcgtcc ttggcgttga agcggacgga cgtcaaccca
      121 aggaaaaggc cgagtccttc gggcaatcga ttcaagaaat atgtgccgcc cacgtccgga
      181 tagaggccga tcgacatctc gggcatcgcc atgatggttc gctcagtaac cactctcgaa
      241 caggcgccgt tcatgagacc gatgccgccc cccatggtaa tgccgtcggc ccaacagaga
      301 accggtttgg agtatctatg gatgaggtaa tccacaaagt actcaacggc aaagaactcg
      361 gcggcgcatt ctagtccacc ggtggttctt agctcgctca caagagtttt tacgtcgccg
      421 cccgcgcaaa acgccttgtc ggagtcagcg tgaaagatga cgcatgcgac atcggactgc
      481 gcctcccatg cgagtagttg ctcttcgatc gcttgaaaca ttcctagcgt gagcgcattg
      541 agagcccgcc tgttgttgag ctcgatcagg ccaatggtat gccgcccggc tttttcgagg
      601 cgaaaatcgg ctccgggcac agagtgaagg cttctcgtca tcctagggta tgatcctatt
      661 catccgggca caaaaatgta aaccgctgac agtccgcggt ggcagagttt gttccacccg
      721 tgcccttgtc aaacgtatgc ttgtatcgct ttcttttttt tatcgattac ttagcgtttt
      781 tatgctacgt gtcagatcgt ggcattgagc ttgcttgacg tcgcactgtt gcaaattacc
      841 gacagtgagg gacaaatgga atcaactata aacgaaagtt cttactccat tcagtacgac
      901 aaggaaacct acaacgcagc tgtttgtcat cgttgcggcg cgcgaatgtt tcccgccgag
      961 ctgctcgaag cccacatgga ccgacatgaa ttaaaggata tgtatttgca aggtgaactg
     1021 aagaaacttc aattcgccat gaaccgcatg cgatagttta gaatgccgac acgatcggcg
     1081 cacgatgtgc ccgagcgcaa gcgtgaattc tcctcctcat tcacgtttgc tgccatcgcc
     1141 ccgggtgtcc tcacaggagg acacccgtcc cccaatcaga agtagttcct tccgctcacc
     1201 ctcgacgctt cattacttag cgaccaaata gccgcggttg agaaccgccg caaccctgca
     1261 acccgcccct tcgccccgca atcgttgcgt tgacttcaaa aatcgctctt gttagccaat
     1321 aacttttgca acaggaggcg ctttgaaagc tgttcgcatg catgggcatg gcggcgttga
     1381 tcaactgtgt ttcgagaatg cagatgagcc tcgatgtgac ggcgcggctc aagccatcgt
     1441 gaagctcagc gccgcggcgc tcaatcacat agatatttgg aatcgcctgg gcgtaaccgg
     1501 cgtgcggata gccatgccgc atattcttgg cgccgacggc gccggagtcg ttgtcgacgt
     1561 cagccaaggc gtcgagcacg taagagttgg cgatcgggtt tgcctctatc cgccgaccgg
     1621 ctgcggccag tgtgaatttt gcgttaccaa tcgagacttc atgtgctttc gcttacgtgt
     1681 gttgggcgag agattggaag gcacgtatgc tcagtatgtc aaactgccgg cgcaaaattg
     1741 ttttccgatt ccaaccggct attccttcga ggaagcagcg gccttcccgc tggtgttcat
     1801 tacgctttgg cgtatgttga tcaccaatgc ccggcttgag cccggcgaaa cgattctcat
     1861 cctcggtatc gggggcggcg tcgcgagcgc ggcgttacag gtggcaaaga aaatcggcgc
     1921 ccgggtgatc gtcacatccg gtagcaatga aaaactcgaa cgcgcgcgcg atttaggcgc
     1981 cgattacgca atcgatcatc gcagcaagga ttttgcccaa gaggctcaaa cggtgaccga
     2041 tggtagggga gtcgacgtcg tgctcgactc tattggcggc gagttttggc ggaagagctt
     2101 ggcctctctt gcgcgtggcg gacgtctggt cacgtgcggc gcaactgccg gcggtcagcc
     2161 catcgatgat attgcggcga tatgtgccaa ggaactaaaa atttatggct caaccctcgg
     2221 cagccgcgaa gactttagtc aggtgatcaa ttttttaaat accgctaaga tcaagccggt
     2281 gatcgatacc atctttccgc tcgagaacac ggccggcgcg cagcttcatc tcgagaacgg
     2341 gcgccaattc ggaaaaatcg ttctccgaat accagactag gcgccaaaca ccgttgacgt
     2401 tatacacatc aagcggcaat gcaaattggc gggagacggc gcatgccatg cgcgcctacg
     2461 attccgcgtg gcaggttgtc gtacgcagtc gtttcaacgc tgcagcaagc tgcgccggtt
     2521 cagcgcctcg gcacaggctt ccagagcacg gcgttgatcg ttggcggcca atcggagaac
     2581 tacagtgcgc gcacctgact cgatatattt ttccatccag tcgatcacct cgtcccagct
     2641 gccagtgaca cagagcatcg cgttggccac cgcctcgtac gagcgattgt aatatttggc
     2701 gaggaaagct tggccttccg cgaccgcttc tttgccgccc gccgcaagag tgaaataaat
     2761 tcctgcgcga atcccatccg gatcgcgccc gagatcgttc gcgtaagcgt ggattttctg
     2821 ccggcattca gcgaatagag cgggactttt gatattgttg atccaggcat cgccgaggcg
     2881 agccacacgc ttcaagccag gttcgacgtt gttcgaagcg atccagatcg gcacaccgcc
     2941 tgcctgcacc ggcttgggct ggatggaaac atcatcaaac ttggtaaatc tacctgcatg
     3001 agagacatga tcttcacgcc ataaacggcg catgatttga attacctctt cgctcatctt
     3061 gatgcgcgcc gccggatcga gaccgaggat ctcaaattca tgctcacccg ccggatcatt
     3121 tggccggcca tagccgacac cgaaatcgat ccgccctccg gtgatccagt cgaggcttgc
     3181 cagctgataa gcgagcacta ccggatgacg caatgccgca ag
//
LOCUS       DASULZ010000509         2717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1381449, whole genome shotgun sequence.
ACCESSION   DASULZ010000509 DASULZ010000000
VERSION     DASULZ010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381449"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VFY96_10445"
     CDS             complement(<1..534)
                     /locus_tag="VFY96_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX5606924.1"
                     /translation="MFEIVEQNSLAAARIKVIGIGGGGGNAVNTMIGSRLSGVDFMVA
                     NTDAQSLQASQAPVRIQLGSMVTKGLGAGANPEIGRRAALEDQEQIREYLEGSDMIFI
                     TAGMGGGTGTGGAPVLARVARDVGALTVGVVTKPFIFEGKKRMHQAEEGIEELKASVD
                     TLIVIPNQRLLSIAAKTT"
     gene            complement(576..1805)
                     /gene="ftsA"
                     /locus_tag="VFY96_10450"
     CDS             complement(576..1805)
                     /gene="ftsA"
                     /locus_tag="VFY96_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX5606925.1"
                     /translation="MGKKNSIVVGLDIGTSKVCAAVGEMTEHGLEIIGIGLHPSQGLR
                     KGVVINIETTVNSLKKAVEEASLMAGCEIHTVFTSISGGHIKGFNSHGIVAVKNKEVM
                     ARDLERVIDAAKAVAIPMDREVLHVLPQDYIIDDQDGIRDPLGMSGVRLEAKVHIVTG
                     AVASAQNVIKCCNRAGLNVAEIVLTPLAAADAVLTDEERELGVVLVDVGGGTTDIAVY
                     HDGTVKHTAVVPIGGNHITNDIAAGLRTAFNDAERLKQRYGCACASMVSSSERVEVPC
                     VASKGAGTISRQILCEIIEPRLAEIFELVQKEVTKSGYEGSLASGIVATGGTMLLAGA
                     VDMADRAFGLPVRLGTPARVGGLVDIINSPIHAAAVGLTLHGVKRQDRSAAFRLREDK
                     ILSKVKHRMSDWLSDFF"
     gene            complement(1826..2641)
                     /locus_tag="VFY96_10455"
     CDS             complement(1826..2641)
                     /locus_tag="VFY96_10455"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEX5606926.1"
                     /translation="MQLTVYRRDNRKKDQHRWRRRLLQIGVVLGAAVLAAAIYRLSGP
                     FGIAAAALYDFVLDNAYFSVREIQVHGGEKVSGSEIVAIAGLHRGMSIWKVDLAAIEK
                     KIAKHPWVRRVLARREFPRRIVIDVQERVPKAIVAIRKLYYVDGDGLVFKEIGPGENV
                     KYPLLTGLRAEELTNSDPLWRRRIQDAIRLGELMARRSHSLSEIHFEAPDRLVVYTTD
                     FPVALRMGWGDWEDKLGRMERLMMLWKGNENRLASLDMSFQDQIVTRLRRVQR"
BASE COUNT          560 a          864 c          698 g          595 t
ORIGIN      
        1 ggtggttttc gctgcaatgc tcaacaggcg ctggttggga atcacgatca aggtatccac
       61 gctcgccttc aactcctcaa tgccttcttc ggcctggtgc atgcgcttct tgccctcgaa
      121 gataaacggc ttggtgacaa caccgaccgt gagcgcgccg acgtcgcgcg ccactcgcgc
      181 cagcaccggc gcaccaccgg tgcccgtgcc accgcccatg cccgctgtga taaagatcat
      241 gtccgagccc tcgaggtatt ctcgaatctg ctcttgatcc tccaaggcgg cgcggcggcc
      301 gatttccgga ttggcgccgg cacccaagcc tttggtcacc atgctcccca gctggattcg
      361 caccggcgcc tggctcgctt gcaaagattg cgcgtcggtg ttcgcgacca tgaaatcgac
      421 acccgagagt ctcgagccga tcatggtatt gaccgcatta cctccgccgc caccgatgcc
      481 gatcaccttg atgcgtgcgg cggcgagact attctgctcc acgatttcaa acatagattt
      541 ccctcctcta aagtcagtaa atttgcttcg cccaattaaa aaaaatcgct cagccagtcg
      601 gacatccgat gcttgacctt cgacaatatt ttgtcctcgc gcaggcgaaa ggcagcgctg
      661 cgatcctgcc gcttgacgcc atgcagcgtg agacctaccg cggcggcatg gatcgggcta
      721 ttaatgatgt ccaccagccc gcccacgcga gctggcgtac ccaaacgaac cggcagacca
      781 aaggcgcggt cggccatatc gaccgcgccg gccaaaagca tcgtaccgcc cgtggcgaca
      841 attcccgacg ctaaggatcc ttcgtatccc gacttggtga cttccttttg cacgagctca
      901 aagatctccg ccaagcgcgg ctcgataatt tcacagagaa tctgtcgcga gatggtgccg
      961 gcccctttgc tggcaacaca cggcacttcg actctctcac tgctagagac cattgacgcg
     1021 caggcgcagc cgtagcgctg cttgagccgt tcggcgtcgt tgaacgcggt gcgtaatccg
     1081 gcggcgatgt cattggtgat atgatttcca ccgatcggca ccaccgccgt atgtttcacc
     1141 gtaccgtcgt gatagaccgc gatatcagtc gttcctccgc cgacgtccac caatacgaca
     1201 ccgagttccc gctcctcatc agtcagcacg gcgtcggccg cggcgagcgg tgtcaaaacg
     1261 atttcggcga cgttgagacc tgcccgattg cagcacttga taacgttttg tgccgaggcg
     1321 actgcgccgg taacgatgtg aaccttcgct tcgagacgaa ctcccgacat ccccaaaggg
     1381 tcccggatgc cgtcttggtc gtcgatgata tagtcctgcg gcagcacatg gagaacctcg
     1441 cggtccatcg gaatcgccac cgctttagcg gcatcgatta cccgctccag gtcccgcgcc
     1501 atcacttctt tgttctttac cgcaacgata ccgtggctgt tgaaaccctt gatgtgaccg
     1561 ccggatatgc tcgtgaacac cgtgtgaatt tcgcagccgg ccatcagact cgcttcttcc
     1621 accgcttttt tcagtgaatt caccgtggtt tcgatgttga tcaccacgcc ttttcgcagc
     1681 ccctgcgatg gatgcaagcc gatcccgata atctcaagac cgtgttcggt catttcaccg
     1741 acggcggcgc aaaccttcga tgtgccgata tccaagccga cgacgatgct gtttttcttt
     1801 cccatagaag attgacttcg atgaattagc gctgcacccg gcgcaaacgg gtgacaattt
     1861 gatcttgaaa actcatgtcc agtgaggcga gacgattttc gttgcccttc caaagcatca
     1921 tcagtcgctc cattcgcccc aatttatcct cccagtctcc ccaacccata cgcagggcga
     1981 ctgggaaatc agtggtatag accaccagcc gatcgggcgc ttcaaaatga atttccgaaa
     2041 gcgagtgcga gcggcgcgcc atcagttcgc caagccggat cgcgtcctgg atccggcgcc
     2101 gccatagagg atccgagttc gtcagctctt cggcgcgaag tccggtcagc aacggatatt
     2161 ttacgttttc gcccggcccg atttccttga acaccaaacc gtcgccgtcc acatagtaaa
     2221 gcttacggat cgcgacaatc gctttcggca cccgttcttg gacgtcgatc acgatgcggc
     2281 gcggaaattc gcggcgcgcc agaactcggc gcacccaagg gtgtttggcg atttttttct
     2341 cgatcgctgc gagatcgact ttccagatgc tcatgccgcg atgaaggccg gcaatggcga
     2401 cgatttcgct accgctcact ttttcgccac catgcacctg aatctcgcgc acggaaaaat
     2461 aggcattgtc caaaacgaaa tcgtaaagcg cagccgcggc aatgccgaac gggccgctga
     2521 gtcggtagat cgctgccgcc agaactgctg cgcctagaac gacgccgatt tggagaagcc
     2581 gacgccgcca ccggtgttga tccttttttc gattatcccg acgataaacc gtgagctgca
     2641 taatctcctg ttacgactcc caatccccga acaatttgac ctcgggtacg agctcgatgc
     2701 cgaattgtct gtgcact
//
LOCUS       DASULZ010000510         4545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1481922, whole genome shotgun sequence.
ACCESSION   DASULZ010000510 DASULZ010000000
VERSION     DASULZ010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481922"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VFY96_10460"
     CDS             <1..504
                     /locus_tag="VFY96_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5606927.1"
                     /translation="RALDFLATIPYAVPHTVMAIAMILAWAKPPLSLYGTLWIILVAY
                     LAIYLPFAVRTTHATLQQVHDSLEEAARVSGAKLLPRLRDVILPLARPGMIAGWILVF
                     MPALRELTVSILLYTHRTETIGVVVYNMQDAGYREIAAALASLVMILLIAGNGLIRKL
                     SGGVAGF"
     gene            509..1576
                     /locus_tag="VFY96_10465"
     CDS             509..1576
                     /locus_tag="VFY96_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015858630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5606928.1"
                     /translation="MEKIRIVNLSKRYGKVVAADHVTLSIGDKEFFSLLGPSGCGKST
                     VLRCVAGLEEPSDGEIYIGETQVNCAAAGINVPTEERPIGMVFQNYAIWPHMTVHDNV
                     AYPLKLKKLPKEMIEAKLKEILRAVGLERLAGRFPSELSGGEQQRVALARALIKEPEV
                     LLLDEPLSNLDAKLREQMRFELKDLQRRTRIPILYVTHDQSEALAMSDRLAVMNAGRI
                     VQIGTPDEIYSHPADRFVADFIGLMNFVPARIVERSGDRVTVEIFGGHRLTISDRKAL
                     SGACILAVRPEDIAMSPQGAIRCRVVVSSYSGHLIDYKISTDGTVLRVQTPKTMTYDE
                     GADLGFTIERAILFGAGEGER"
     gene            complement(1800..2105)
                     /locus_tag="VFY96_10470"
     CDS             complement(1800..2105)
                     /locus_tag="VFY96_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX5606929.1"
                     /translation="MGTKLYVGGLPYSVTEGRLQEIFSAHGTVQSTNVIADKFTGQSR
                     GFGFVEMSSGSEAQDAISSLNGTQLDGRTLTVNEAKPMSRRDDGGGRGNRQGGLNRW"
     gene            2857..3798
                     /locus_tag="VFY96_10475"
     CDS             2857..3798
                     /locus_tag="VFY96_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002635830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX5606930.1"
                     /translation="MRFAANSFQVNFRVARRLDEVAEILAAQGANPYRVRAYRRAADT
                     VRYLHGSIAEILREQGEPGLRTLPGIGESLARSIATLLMTGSLPMLNRLRGETDSESL
                     LASVPGIGKQLAGRLRRDLRIHTLEQLEAAAHDGRLKDIAGIGEKKLAGIADSLATRL
                     GRVRPQRHSDSEKSPEPGVEEILDVDREYRQKATAGTLPKIAPRRFNPNREAWLSILH
                     SERGSRHYTALFSNTARAHEKGKTTDWVVLYCETKDGEQQYTVITSERGPLLGKRIVR
                     GRELECMEYYYRQGKILLPEVENQASVPQPYKQPAVA"
     gene            complement(4071..>4545)
                     /locus_tag="VFY96_10480"
     CDS             complement(4071..>4545)
                     /locus_tag="VFY96_10480"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5606931.1"
                     /translation="VYGVPYGAYSAGEGFYDPTQLDKFVAQLRNEAEEYLEQKAAELK
                     GHGVDKVSYDAKEGIDADEIISFARHLADNLIVMCTHGRSGINRWVLGSVTETVVRHS
                     DNPVLVIRGPGDREFARTAARKQRYHWNSHPPLAAAMFALQGTQIPNLFSRYVDG"
BASE COUNT         1019 a         1231 c         1261 g         1034 t
ORIGIN      
        1 cgagctctgg attttttggc gacgattccc tatgcggtac cgcacaccgt catggccatc
       61 gccatgattc tcgcctgggc caagccgccg ctcagcctgt atggtacgtt gtggatcatt
      121 cttgttgcct acctggcgat ctatttgccg tttgccgtgc gtacgaccca tgcgactttg
      181 cagcaggtgc acgattcgct cgaagaagcc gcgcgcgttt cgggggcgaa gctgctgccg
      241 aggttgcgcg atgtgatctt acccttggcg cggccgggaa tgatcgccgg gtggatactc
      301 gttttcatgc cggcgctccg cgaactgaca gtttcgattc ttctttatac tcatcgcacc
      361 gaaaccattg gcgtagtggt ttacaatatg caggatgccg gttatcgcga gattgccgcc
      421 gccctcgctt ctctcgtcat gattctcctc atcgcaggca acggtctcat taggaaactc
      481 agcggcggag tcgccggttt ttagtcctat ggagaagatc cgcatcgtca atctcagcaa
      541 gcgctacgga aaggttgtcg ctgccgatca tgtaactctc tccattggcg ataaggagtt
      601 tttttctctg ctgggcccga gcggctgcgg caagagcacc gttttaaggt gtgtcgctgg
      661 tctggaggaa cctagcgacg gagaaattta cattggcgaa actcaggtga attgtgcggc
      721 cgcaggcatc aatgttccca ccgaggagag gcctatcggc atggtgtttc aaaattatgc
      781 gatctggcca cacatgacag tgcacgacaa tgttgcctat cccttgaagc tcaagaagtt
      841 acccaaagaa atgattgagg caaagctcaa agaaattctc cgggcggtcg gtcttgaacg
      901 gctggcgggg cgcttcccga gcgagctttc gggcggagaa caacagagag tcgcgttggc
      961 gcgggccttg atcaaggagc ccgaggtact gctgctcgat gagccgctga gcaatctcga
     1021 tgctaaactg cgtgagcaga tgcgcttcga gctcaaagat ttgcagcggc ggacccgaat
     1081 tccgatcctt tacgtgacgc acgaccagag cgaagcgctg gcgatgtcag accgtctcgc
     1141 cgtcatgaat gccggccgca tcgtgcagat cggtactccg gatgagatct attctcaccc
     1201 ggcggaccgc ttcgtcgcgg actttatcgg tcttatgaat ttcgtgccgg cgcggattgt
     1261 cgagcgaagt ggcgaccgag tgacggtaga aatctttggt ggtcaccggc tgacaatttc
     1321 cgatcgcaag gctctgagcg gcgcgtgtat cctggcggtg cgcccggaag acattgctat
     1381 gagcccgcag ggcgcgatcc gctgtcgggt tgtggtcagc agctactcgg gacatctgat
     1441 cgattacaaa attagcacag acggcaccgt gctgcgggtg caaacaccga agacaatgac
     1501 ctacgatgaa ggcgccgacc taggtttcac gattgagcgc gcgattcttt tcggcgcggg
     1561 cgagggcgag cgctagtgtc ctagtttcga gtcagcaatg aaaaaacatg cttcttgttc
     1621 ggtcagaacc ccagaacgtt acgcgcgcag ccaagcaccc tcacgccggc cgagtggcta
     1681 acaatgaagt gattaactgg ttcgccccgc attgcaatca tgcgcagatt aaatattgga
     1741 agtttgaaaa gcgggcaacc cgacctgttg cccgatcgat tagctaaacg acaaagagtc
     1801 taccagcgat tgagtccgcc ttgacgattt cctcgcccgc cgccatcatc gcgccgggac
     1861 atcggtttgg cttcattgac ggtcagcgtc cggccgtcga gctgcgtgcc gttcaacgaa
     1921 ctgatcgcat cctgcgcttc gctgcctgaa ctcatctcta cgaaaccgaa tccgcgcgat
     1981 tggccggtaa atttatcggc gatcacatta gtcgattgca ccgtgccatg tgccgaaaag
     2041 atttcttgca gtcgcccttc ggtaaccgaa taaggcagac cgccaacgta tagttttgta
     2101 cccatgttct cctcctaatt aagagaatgt tttgtcttaa accctgagaa ttagactgcc
     2161 agtcggcaga acatgggggg attcaatgat cgaaaccacg ctctggtcgg tttgtccgta
     2221 ggaatcgttc gcaaggagag agacggatta caggccttcg aggtatttct tcccatttca
     2281 gaaggccatg tactgaagta ggtgcagtca atctacgcat caataccagc aatgtcaatc
     2341 atcggacgga tgttttatcg gcctttcgct gcacattggc tgcctgctta tgtcattttg
     2401 ccggttatgg cttaggaacc atccgtcgga gttgatgaca gcgcttcgcg caagctacga
     2461 caatgtcccg gatctctgac tggatgcgag cttgctccca tccggagttg ccgcgctcgt
     2521 tggaggtgcg cagtcattac tttaaacccg gcgagaaact cctggatatg cagaggacgc
     2581 gtcgactttg acgcggcatc cattcgatat tcacaagccg cgccttcggt gccgcggaag
     2641 aagccgaaga ttgcgcacgc tcgaggcgcg cgtgcattga caacctgcac tgcaatatca
     2701 aaaaccctga gtcactctaa cagccagtaa cgtcggtttt gcaggtggca cacacattgc
     2761 cacgatttat caggtacaga tctggaacgc ttttgggttt gaggcactta ttgccagtgg
     2821 aacggctgtt taacggctaa ccgcgagtca ggcgaaatgc gatttgctgc caactccttt
     2881 caggtaaatt tccgggttgc acgccgcttg gacgaggttg ccgagatcct cgccgcgcaa
     2941 ggagccaatc cctatcgcgt gcgagcctat cggcgcgccg ctgatacggt gcggtatctt
     3001 cacggttcca ttgcagagat cctgcgcgaa caaggcgaac cggggttgcg cacattgccc
     3061 ggtatcggtg aaagcttggc gcgctccatt gccacgttat taatgaccgg cagtctgccg
     3121 atgcttaatc ggctgcgcgg cgaaacggat tccgagtccc tactcgcgtc tgtcccgggg
     3181 atcggcaaac aactggctgg gcgactgcgc cgtgatctca ggattcatac tttggaacaa
     3241 cttgaggcgg ctgcacacga cggccgcttg aaggacattg ccgggattgg cgaaaaaaaa
     3301 cttgccggca tcgccgattc gttggcaaca cgcttgggcc gtgttcgtcc acaacggcac
     3361 agcgatagtg aaaaatctcc ggagccaggt gtcgaagaaa tactggacgt ggatcgagaa
     3421 tatcgacaga aagccaccgc tggcactctc ccgaaaattg cgccgcgtcg gttcaatccg
     3481 aaccgggagg cctggctttc gatcctgcac tcggaaagag gtagccgcca ctacaccgcg
     3541 ttgttttcca acacggcgcg agcgcacgag aagggcaaaa ccacagattg ggtcgtgctc
     3601 tactgcgaaa cgaaagacgg cgaacaacaa tacaccgtga ttactagcga gcgcggcccg
     3661 ctgctgggca aacggatcgt gcgcggtcgt gagctagaat gtatggaata ctactaccgg
     3721 caaggcaaga ttctacttcc cgaggtcgaa aaccaagcca gcgtcccgca gccctataaa
     3781 caaccggccg tagcttaaac gcgccgatag tataccggtg ccatgcaaaa acggttgaag
     3841 gtaagtcttg taaaaggaaa atggatcagc aggagaagaa ttaacatccc cctttgcgct
     3901 atgctcgcgc aacgacgttt tccgtcgagg cacgttttct atttgctcga cggcccatga
     3961 gccgtacgtg gttggtaatt tttcccaaag agctgttgag accgctgatt cgattcttcg
     4021 ccaaattttg atgaccttga taaaagtggt ggcatcggga actggaccca tcaaccgtcg
     4081 acataacgac tgaataggtt gggtatctga gtaccctgga gcgcaaacat cgcggcggcg
     4141 agcggtgggt ggctattcca atgataacgt tgttttctcg cagctgtccg cgcgaattcc
     4201 cgatcgccag ggccacgaat aaccaaaacc ggattgtcgg agtggcgcac aaccgtttcg
     4261 gtcacgctgc ccaagaccca tcgatttatt ccggaacgtc cgtgggtgca catgacgatt
     4321 aaattgtcgg caagatgacg tgcgaatgag atgatctcgt cggcgtcaat cccctccttc
     4381 gcatcgtaag aaactttgtc aacgccatgg ccttttagct cggctgcctt ttgctccagg
     4441 tattcttcgg cttcatttct aagctgagcc acgaatttgt ccagttgcgt gggatcgtaa
     4501 aatccttctc cagcagaata agccccgtaa ggaacgccat agaca
//
LOCUS       DASULZ010000511         5617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_467748, whole genome shotgun sequence.
ACCESSION   DASULZ010000511 DASULZ010000000
VERSION     DASULZ010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467748"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VFY96_10485"
     CDS             <1..370
                     /locus_tag="VFY96_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606932.1"
                     /translation="LVARHLNEDGLFIFDVNTSGKLHRLAAGPAWEKWFGRHLAIIRV
                     NAQRAGRFEWDVKIFAHESRDDYKLLHEKIQEIALPKRQILESLRRQFRQVKALDPFG
                     FRASDQSERLYFVGKTPRFF"
     gene            470..1642
                     /gene="argJ"
                     /locus_tag="VFY96_10490"
     CDS             470..1642
                     /gene="argJ"
                     /locus_tag="VFY96_10490"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX5606933.1"
                     /translation="MQQTRADEFAHPQGFLSLAKNVGIKDSSLDLAVIYSTVRARAAA
                     MFTRNRFPGAPVIVGRKHIARGFVQAMVVNSKNANVAMGKQGIDNAIETCRIVGEQLS
                     INPYDVLPFSTGVIGRPLPMDKIRAGLKGIRSELKPDNLQLAAEAIMTTDTSPKYYSC
                     PVGQAVIAGIAKGAGMIEPNMATMLVYLMTDAELPKAALRPMLRRVVNRTFNCVSIDT
                     DTSTSDTVVLMANGLAGTVKLGGFEKALLGVCEHLTKQIARGGEGATKLITVDVSRAK
                     NDIQAKRVAKCVVNSPLVKTAVYGCDPNWGRVIAAVGKSFDPSIEPNKVTIRFGRTEV
                     FKKGSPVACDLEALREYLGQSEVMITVELGLGKASARVWGCDLTEGYIKENAYYTT"
     gene            1956..3128
                     /locus_tag="VFY96_10495"
     CDS             1956..3128
                     /locus_tag="VFY96_10495"
                     /inference="COORDINATES: protein motif:HMM:NF006689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HEX5606934.1"
                     /translation="MIRQLPNFILKAGRVIDPASGTDGIFDIRVRDGKLDAIGVDLAP
                     DGAAVIDVKDHIVTPGLIDVHLHLMKGLGAFGVDPDIFGVGSGVTTVVDAGSAGHSLL
                     NVFRNYVTDNAKTRVLNYINLSTLGGVSGPGYSILADSRLIDEAKIEQAVEANRDIIV
                     GIKIMATGGALGAQGLKPLERARKLGDALKLPLLVHIGESWTKDTEPVHVGDVLKYLR
                     AGDIVTHMFTVHPGGLLDSNGKLWPQVREAKESGVLMDVGHGLHNLNFDVARKVLDQG
                     LHPDGVSTDGHRGNRAGPVYDLPTTMAKLMALGFSLNQVIEMATVNAAKLLGRAGEIA
                     ALKLDHPAELSVLRIEEREWKAVDSQKGTIPAHQAITPVYAIRGEMIHVAVPAERP"
     gene            complement(3132..4589)
                     /locus_tag="VFY96_10500"
     CDS             complement(3132..4589)
                     /locus_tag="VFY96_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX5606935.1"
                     /translation="MPEGLPIAKGSDLQLLLPAMANRHGLIAGATGTGKTVTLRVLAE
                     HFSNIGVPVFMADVKGDLSGMARRGQENPKIKERLIQLGLDDFQFASFPTVFWDVYGQ
                     QGHPVRTTVSEMGPLLLSRLLNLNEIQSGVLQLVFKIADDNGLLLLDLKDLRAMLQNV
                     GDNAAQFKTQYGNISAASIGAIQRGLIALDEQGGDKFFGEPALDLDDFVQTDSTGRGM
                     VNILVADHLINAPKIYATFLLWLLSELFERLPEVGDPEKPKLVFFFDEAHLLFSDAPR
                     VLMEKVEQVIRLVRSKGVGVYFVTQNPLDVPDIVLGQLGNRVQHALRAFTPRDQKAVK
                     AAAQTFRANKKIDVEKAITELGVGEALVSFLDEKGQPNPVDRAMIFPPQSQLPPLAGA
                     EREQVIRQSTLYGHYEKIVDRESAYEMLKVRAPQAGAEQAGSQSGVSWQSEAGSLLEA
                     AGKSAVRAMGTQLGREIIRGVLGSILGSTSTRKKR"
     gene            4805..5113
                     /locus_tag="VFY96_10505"
     CDS             4805..5113
                     /locus_tag="VFY96_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606936.1"
                     /translation="MMKRQLGLILAVLFLSASFALAKDYQVTGPVVDVKDDAIIVKKG
                     NENWEIARDKDTKTSGEVKKGDRVMVKYKMIATTIEGKEPAKGKSDTKATSDTKSKTK
                     "
     gene            5245..>5617
                     /locus_tag="VFY96_10510"
     CDS             5245..>5617
                     /locus_tag="VFY96_10510"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5606937.1"
                     /translation="MPAHPEVTIVHSSDLHLGTDDSFSDKDRLAALPRVLSAASEVKA
                     DVVVLAGDSFDNHRQPLELLERAAGMLRDYSRPVVILPGNHDPLTPDSVYRRGGLGLI
                     PNVTILGLNVEKAALFDEFELE"
BASE COUNT         1316 a         1527 c         1529 g         1245 t
ORIGIN      
        1 tcttgttgcc agacatctga acgaggatgg actctttatt tttgatgtca acacttctgg
       61 caagctgcat cgtctcgccg cgggtcccgc ctgggaaaaa tggtttggtc gccatctggc
      121 aatcatcaga gtcaacgcac agcgagctgg gagattcgag tgggatgtca aaattttcgc
      181 gcacgagagc cgggacgatt acaaattgct gcatgagaaa attcaagaaa tcgcgcttcc
      241 gaagcggcaa attttagaat ctctgcggcg tcaattcagg caagtcaaag ctctcgatcc
      301 gttcggtttc agagcttcgg atcagtcgga gcgcctctat ttcgtcggca aaacgcctcg
      361 ttttttttga ccggcggagt acgtatcatg tcagtctatt ccgctcgatg cttttctcgt
      421 gctaacctct gcggcaaact ggaaatgatt taggagtcac gcgtagacaa tgcagcaaac
      481 ccgtgccgac gaatttgccc atccgcaagg ttttctatcc ctggcgaaaa acgtgggcat
      541 caaagattcg agcctcgatt tggccgtcat ttattccacc gtgcgcgctc gcgcggcggc
      601 gatgttcacg cgcaatcgtt ttcccggcgc gccggtgatc gtcgggcgaa agcacatcgc
      661 cagagggttc gtccaggcga tggtcgtcaa tagcaaaaac gccaatgtgg caatggggaa
      721 acaaggcatc gacaatgcca ttgaaacctg tcgcatcgtc ggagaacaat tgagcatcaa
      781 tccttacgat gtgctgcctt tttcgacggg tgtgatcggg cggccgctgc cgatggataa
      841 aatccgcgcc ggcctaaaag gcattcgcag cgagctcaag cccgacaatc tccaactcgc
      901 cgcggaagcg attatgacca ccgacacatc gcccaaatat tattcctgtc cggttggcca
      961 agcggtgatt gcgggaatcg ctaaaggcgc aggcatgatt gagccgaata tggcgacgat
     1021 gcttgtttat ctaatgaccg atgccgagct acccaaagcg gcgctccgac cgatgctgcg
     1081 ccgagtggtg aaccgaacgt tcaattgcgt gagcatcgac accgacacga gcacgagcga
     1141 caccgtcgta ttgatggcca acggccttgc cgggactgta aaactcggag gattcgaaaa
     1201 agctctgctc ggcgtctgcg aacatctgac caaacagatc gcgcgcggcg gcgaaggcgc
     1261 gaccaaactg attaccgtcg atgtaagccg cgccaaaaac gacatacagg caaaacgtgt
     1321 ggccaaatgc gtggtcaatt cccccttggt gaagaccgca gtttatggct gcgatcccaa
     1381 ttggggtcgt gttattgcgg cggtagggaa gtctttcgat ccaagcattg aaccgaacaa
     1441 agtcacgatt cgtttcggca gaaccgaagt attcaaaaaa ggctcaccgg tcgcatgcga
     1501 tctcgaggcg ctacgagagt acctcggcca atccgaagtg atgatcacgg tggaattggg
     1561 acttggaaaa gcctcggcgc gggtctgggg atgcgatcta accgaaggtt acattaaaga
     1621 aaatgcctac tacacgacgt gaatcgaaag ctagcggctt cacagccgca cggaaacgaa
     1681 gggcgcaacc gggattccga gcccgtggat taccgctaaa tacacgcggg aatgacagtc
     1741 tcgaaacgga accagttctt tgaaaagact ctatgaaaaa tagcattgcg ggcccgaaat
     1801 cccccggtcc tgcggcccgc cccctttgaa aaagggggcc atgggggatt tatccgtgct
     1861 gctccgaacg cactgcagct cattttcatt ctgtgcgttg tacagattgg tacatgaaat
     1921 actccggtgg aaaatcagcg acaaagtaac aagcgatgat tcgccaattg cccaacttca
     1981 ttcttaaagc cggccgagtg atcgatcccg cttctggcac ggacggcata ttcgatatcc
     2041 gcgttcgcga cgggaaactc gacgccatcg gcgttgatct cgctccggac ggcgcagcgg
     2101 tgatcgacgt taaagaccat atcgtcaccc ctgggttgat cgacgttcat ctccatctca
     2161 tgaaaggcct tggagccttt ggcgttgatc cggacatttt cggagttggc tcgggagtga
     2221 ccacggttgt tgatgccggc agcgcgggac atagcctgct caatgtcttt cgcaattacg
     2281 tgacggataa tgccaagacc cgcgtgctca actatatcaa tctctcaacc cttggcggcg
     2341 ttagcggtcc cggctacagc attttggccg attcgcgctt gatcgatgaa gcgaagatcg
     2401 agcaagcggt cgaagcgaat cgcgatatca tcgtcgggat caaaatcatg gcgacgggag
     2461 gagcgctggg cgcgcaggga ttaaagccat tggagcgggc gcgcaagctc ggggatgcac
     2521 tcaaattgcc tttgttggtc catatcggcg agagctggac caaagacaca gagccggtgc
     2581 acgtgggtga tgtcctgaag tatttgcgcg ccggtgacat tgtcacgcac atgttcaccg
     2641 ttcatcccgg gggattgctt gacagcaacg gtaagctctg gccgcaggtg cgcgaagcca
     2701 aagaatctgg cgtgctcatg gacgtcggcc atgggctgca caacctgaac ttcgacgtcg
     2761 cacgcaaagt gctcgatcaa ggtctccatc cggacggagt ctcgacggac ggtcatcgcg
     2821 gcaatcgcgc cggtcccgtt tacgacctgc ccacgaccat ggccaaattg atggcgctcg
     2881 gattttcttt gaatcaggtg atcgagatgg ccacggtcaa tgcggcaaag cttctgggcc
     2941 gcgccggcga gatagccgcg ctgaagcttg accacccggc ggagctttca gttttaagaa
     3001 tcgaagagcg cgaatggaaa gccgtcgatt cacagaaggg aacgatcccc gcgcatcagg
     3061 cgatcacgcc ggtgtacgcg atccgcggcg agatgattca tgtcgccgtg ccagctgaac
     3121 gaccgtaaac tttagcgctt tttcctcgtg ctcgtgctgc cgagaataga tccaagcacg
     3181 ccgcgaataa tttctcgacc caactgcgtg cccatcgccc gaaccgcgct tttcccagcg
     3241 gcttctaaga gagacccggc ctccgactgc cagctcacgc cgctttgaga accagcttgc
     3301 tcggctccag cttgcggtgc tcgcaccttg agcatctcat atgccgactc gcggtcgacg
     3361 attttttcgt agtggccata aagagtcgac tgtctgatca cctgttcacg ttccgcaccg
     3421 gcgagcggcg gcaattggct ttgcggcgga aatatcattg cgcgatcgac ggggttcggc
     3481 tgacccttct catcgagaaa agaaaccagg gcctcgccga cgccaagctc cgtgatcgcc
     3541 ttctccacgt cgattttttt gttcgcgcga aatgtctggg cggcggcctt gaccgctttc
     3601 tgatcgcgcg gtgtgaaagc gcgcaaggcg tgctgcaccc gattgccgag ctggccgaga
     3661 acaatatcgg gtacgtccaa tggattctgc gtaacgaaat acacaccgac tccctttgag
     3721 cgcaccaacc ggatcacctg ctcgactttt tccatcaaca cgcggggggc atcgctgaaa
     3781 agcagatgcg cctcgtcgaa gaaaaacacc agcttgggtt tttccggatc gccgacttcg
     3841 ggaaggcgtt cgaacaactc cgaaaggagc cacagcaaaa aagtggcata gatcttcggc
     3901 gcattgataa ggtgatcagc gacgagaata ttgaccatgc cacggcctgt ggaatcggtt
     3961 tgcacgaagt catcgagatc gagtgccggc tcgccgaaaa atttgtcgcc gccttgttca
     4021 tcgagcgcta tcagcccacg ctgaatcgcg ccgatgctgg cagcagaaat atttccgtat
     4081 tgggttttga attgtgcagc gttatcgccg acgttttgaa gcatggcgcg gagatctttc
     4141 aaatcgagca ataggagtcc gttgtcgtcg gcaatcttga ataccagctg taacactccg
     4201 ctttggatct cgtttaagtt gagcaagcgg gacaacagca gcggtcccat ctcggaaact
     4261 gtcgttcgca ccgggtgccc ttgctgtcca taaacatccc agaatacggt cggaaagctg
     4321 gcgaattgaa agtcatccag gcctagctgt atcaatcgtt ctttgatctt gggattctcc
     4381 tggccacgtc gagccatccc cgaaaggtcg cctttgacat cagccataaa gacgggcacg
     4441 ccgatattgc tgaaatgctc ggcaagaacc cgtaacgtga cggtttttcc agtccccgtt
     4501 gctcccgcga tgagcccgtg acggttcgcc atcgcgggca gcaataactg aagatcgctt
     4561 cctttggcga tcggtagacc ttctggcatc gcgttactcc ttaattactg ggctccttac
     4621 cagatcttcg gtaatgattc cgcttggaaa aatcaattcc ttgtataacc cagcgccagg
     4681 gtgaatgaaa ctcggtatga ggcgtttccc aatgtgaatt tctccgctgc cgtcgcgtct
     4741 aactatcgag ttttcgtgga ggtattattt ttccgccaca aatttctaac aaccgagggg
     4801 gtttatgatg aagcgtcaat tagggctaat tttagcggtg ctatttttga gcgcttcttt
     4861 cgctctagcg aaagattacc aagtcaccgg gccggtggta gacgtcaaag acgacgctat
     4921 catcgtgaag aaaggcaatg aaaactggga aattgcccgc gataaggaca caaaaaccag
     4981 tggtgaagta aaaaagggcg accgggtgat ggtcaaatac aagatgatcg ccacgactat
     5041 tgaaggcaag gaacccgcga aaggcaaatc cgacaccaag gcaaccagcg acactaagag
     5101 caagactaag taactcgacc ttatatccta cccctccacg tgatctcgga cccggatcgc
     5161 ccagcgaccc gggtctcttt ttgctcacct tttcttttgt atgcgctttt gttaggttct
     5221 taaagtcatc ggcgaaaact ctacatgcct gcacatccgg aagtcaccat cgttcatagc
     5281 tccgatctgc atctcggcac ggacgatagc ttcagcgata aagatcgtct cgcggcactc
     5341 ccgagagtct tatcggctgc cagcgaagtg aaagccgatg ttgtcgtgct tgcgggcgat
     5401 tcctttgata atcaccgcca accgctggaa ctgctcgaac gcgccgcggg catgctccgt
     5461 gattacagtc gcccggtggt cattcttccg gggaaccatg atccgctaac gccggattcg
     5521 gtttaccgtc gcggcggcct aggcctgatt ccgaacgtta cgattctcgg attgaacgtc
     5581 gaaaaagccg cactattcga tgaatttgag ttggaga
//
LOCUS       DASULZ010000512         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_771732, whole genome shotgun sequence.
ACCESSION   DASULZ010000512 DASULZ010000000
VERSION     DASULZ010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771732"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VFY96_10515"
     CDS             <1..264
                     /locus_tag="VFY96_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606938.1"
                     /translation="TLPFLVFTMNHFAPPAPMIAAIGNGESPSAIDSTTSDDSSGIVN
                     TGTAVFSCGDWLDAPRASVRLKEIIPTKISAKARAVDSGTSLQ"
     gene            complement(391..1656)
                     /locus_tag="VFY96_10520"
     CDS             complement(391..1656)
                     /locus_tag="VFY96_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5606939.1"
                     /translation="MVEPNESLDQRLFTHCCIAGGGPAGMMLGFLLARAGVDVIVLEK
                     HADFLRDFRGDTIHPSTLEIMYELGLLDEFLKLPHQKVYQLSAQIGDTQLNLADFREL
                     PVRCPFIAFMPQWDFLNFLADSARRYPTFRLLTQAEVTDLVNASDPVTGVRVKTPQGT
                     IHVAADLVVGADGRNSLIREKARLPVQEIGAPMDVLWFRLPRNPNDPAQTMGRFDTGR
                     IFISINRGEYWQCGYVIAKGRFDWIRRQGLEAFRKEIAQLAPYAKDRVDELKAWEDVK
                     PLTVRVDRLSQWFGPGLLCIGDAAHAMSPIGGVGINLAVQDAVATANALARPLYDRRV
                     RIKALARVQKRREFPTRLTQWLQVAMQRRVIARVLRQTGPVKPPLLLQLIAAIPLFRR
                     ITARIIGLGIRPEHVRVPEWHAGTGKSAT"
     gene            1838..>2033
                     /locus_tag="VFY96_10525"
     CDS             1838..>2033
                     /locus_tag="VFY96_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606940.1"
                     /translation="MRKLLVFQHVAHEILGTLNPLLKRAGFRIRYVNFGRHPDAQPSL
                     DGYHELVILGGRRVFTKIIAS"
BASE COUNT          443 a          598 c          560 g          432 t
ORIGIN      
        1 acgttaccgt tccttgtctt taccatgaac catttcgcgc caccggcgcc gatgatcgcc
       61 gccatcggca atggagagtc tccgtctgca attgactcca ccaccagcga tgattcgtcc
      121 ggcatcgtaa acaccggtac ggccgttttc tcctgcggcg actggctcga tgcgccgcgg
      181 gcatcagtaa ggctcaaaga aatcatccct accaagataa gcgccaaggc gcgcgccgta
      241 gacagtggaa ccagcctgca gtaaccgcgc gccgcagctc gcctaaagct ggtcaatcgt
      301 aaactgtgag gcatgatcct aatcccttcc tgaagtcgtt ggttgacatc gccgggtaag
      361 acccgggcga tttattgagc ctgatcccac tcacgttgca gattttcccg tgcccgcgtg
      421 ccattcgggg actctcacat gctcagggcg aatgcccagt ccgataatgc gcgcggtgat
      481 gcgccggaat agcggaattg ctgctatgag ttggagcaac agcggcggct tcaccggtcc
      541 ggtttgtcgt aacactcgtg caatcactct gcgctgcatg gccacctgta accattgtgt
      601 cagccgcgtt ggaaattcac ggcgcttctg aacccgcgca agcgccttta tgcggacacg
      661 cctgtcgtaa agcggtcggg caagcgcatt cgccgtcgcc actgcgtctt gaacagcgag
      721 attgattccg actccgccga tgggcgacat ggcatgggcg gcatcgccga tgcagagcaa
      781 gcccgggccg aaccattggg acaagcgatc gacgcgaacg gtcaagggct ttacgtcctc
      841 ccaggccttg agctcatcga cgcggtcttt ggcgtagggc gctagttggg caatctcctt
      901 gcgaaacgcc tccaggcctt gtctgcgaat ccaatcgaac cggcccttgg cgatgacgta
      961 gccgcactgc cagtattcgc cgcgattgat gctgataaaa attcggccgg tatcaaagcg
     1021 acccatggtt tgtgccggat cgttcgggtt gcgtggcagg cgaaaccaga ggacgtccat
     1081 gggcgcgccg atttcctgca ccggtaatcg agctttctcg cgaatcagcg aattccggcc
     1141 gtcagccccc accaccagat ctgccgccac atggatggtt ccttgcggtg tcttcacgcg
     1201 aacgccggtt acaggatctg aagcgttaac caggtccgtc acctcggcct gcgtcaataa
     1261 tcggaatgta ggataacgac gcgccgaatc ggcaaggaaa ttcaagaagt cccactgcgg
     1321 cataaaagcg atgaacggac agcgcaccgg caactccctg aagtcagcca agttgagttg
     1381 ggtgtcgccg atttgagcag agagttggta aactttttga tgcggcaatt tgagaaactc
     1441 gtcaagcaag ccgagctcgt acatgatttc aagtgtcgat ggatgaatgg tatcgccgcg
     1501 gaagtcgcgc aagaaatccg cgtgtttttc cagcacgatg acgtcgactc ccgctcgggc
     1561 aagcaaaaac ccgagcatca tgccggctgg accgcctccg gcgatgcaac agtgcgtgaa
     1621 cagcctctgg tcgaggcttt cgttcggctc aaccattgcc agtcacccgc gctaaacagc
     1681 ttccattctc aaatgatgaa ctccgacgat cacctcctga cgattataag cgttcggcat
     1741 cctaaacgac agcagctcca gatcgggcgg ggttggtttg aatgtagcgc aagggcagct
     1801 ataatttttg cgaggaccgg acccatgtcg taatcctatg agaaagctct tggtcttcca
     1861 gcatgttgct cacgaaattc tcggcacttt aaacccactg ttgaagcgcg ccggctttcg
     1921 tattcgttac gtcaacttcg gacgccaccc ggacgcgcaa ccgagcttgg acggatatca
     1981 cgaactcgtg atacttggcg gccgacgggt gtttaccaag atcatcgctt cca
//
LOCUS       DASULZ010000513         2549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1482131, whole genome shotgun sequence.
ACCESSION   DASULZ010000513 DASULZ010000000
VERSION     DASULZ010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482131"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VFY96_10530"
     CDS             complement(<1..396)
                     /locus_tag="VFY96_10530"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX5606941.1"
                     /translation="MLLAIDIGNTNIVLGLYQGKKLITHWRLATQAERTADEYGVIIS
                     QLIDYSGHRSQQISAIAVSCVVPPMVTMTEELGQKFFHIDPLIVGPGVKTGMPILYES
                     PKDVGADRIVNGIAAYDKYRDACIIVDFGT"
     gene            complement(409..1203)
                     /locus_tag="VFY96_10535"
     CDS             complement(409..1203)
                     /locus_tag="VFY96_10535"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX5606942.1"
                     /translation="MTPLDIDQIAKQLKAKRLGGKFHYFAELGSTNIHARMLAEQGAA
                     EGEFVIAEAQTHGRGRLGRRWESPPGVNLYFSVILRPRFLPVHAPQTTLMAAVALAET
                     IVPLIEQAPVIKWPNDILVNGRKLAGVLSEATCDSERIEFVILGIGLNLNYRAVSMPP
                     EIRRRAISVVDLIGKPINRESVTVRLIQNLDRCYGELEDYGFEALRSRWNAFFGLRGR
                     RIRIENHEQVVIGRALGIDRNGALIIEDDFGKLQNIYAGDVIPLES"
     gene            complement(1231..2082)
                     /gene="nadC"
                     /locus_tag="VFY96_10540"
     CDS             complement(1231..2082)
                     /gene="nadC"
                     /locus_tag="VFY96_10540"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX5606943.1"
                     /translation="MDIHVSPQIERLIRDALDEDIGAGDLATMATISADSRGTGVFRA
                     KKEGVVAGLVLLDRIFYFIDHRVELRLHTKDGAEVTPGMVVAEAVGPVRALLLAERTA
                     LNFLQRLSGNATLTRKYVEAVKNYPCKVLDTRKTTPGLRTLEKYAVRMGGGMNHRIGL
                     YDAALVKDNHIEATGSIAKAVKAVRRHAPFMAKVEVEASNLQQVREALDAGADVIMLD
                     NMTLAQMTEAVELVNKRAWIEASGGITLETIRQVAETGVDFISSGALTHSAPVVDFNM
                     KITLNTN"
     gene            complement(2087..>2549)
                     /locus_tag="VFY96_10545"
     CDS             complement(2087..>2549)
                     /locus_tag="VFY96_10545"
                     /inference="COORDINATES: protein motif:HMM:NF021941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX5606944.1"
                     /translation="TVRAIRNLRTEMNCPPSKEVKVIFCGAREDLGFLREQEPYLRSL
                     ARVSESEFRDGSERPKGAATAVIGTTEIYLPLDDLIDLNEERARLSKEVDKIEDELAR
                     VQKKLGNADFIAKAKSEVVHKEREKATRFEEKIRALRANLDKLQEIQAERN"
BASE COUNT          534 a          741 c          660 g          614 t
ORIGIN      
        1 cgtgccaaaa tccacgatga tgcaggcgtc gcgatatttg tcgtatgccg cgataccgtt
       61 cacgatccgg tcagcgccga catccttcgg actctcgtaa aggatcggca tgcccgtctt
      121 cacgccgggc ccgacaatca gcggatcgat gtggaaaaac ttttggccga gctcctcggt
      181 catggtgacc atcggcggga caacacaaga gaccgcaatc gcggagattt gctggctccg
      241 gtggccggaa taatcgatca gttgagatat gatgacgccg tattcgtccg ccgtgcgttc
      301 ggcctgcgtg gcgagccgcc agtgcgtaat cagctttttg ccctgataaa gtccaagaac
      361 aatgttggtg ttgccgatat caatggcgag aagcatggga tttgagcttc agctctccag
      421 cggaattacg tctccggcat agatattttg cagtttgccg aagtcatctt caataatcag
      481 cgcgccgttt cggtcgatgc cgagggcgcg accgataaca acttgttcgt ggttttcgat
      541 ccgaatgcgt cggccacgca agccgaaaaa tgcattccag cgcgaccgca gcgcttcgaa
      601 tccataatct tcaagctctc cataacaccg gtcgaggttc tgaatcaatc gtaccgttac
      661 gctttcgcga ttgattggct tgcctatcaa atcgaccacg gaaatggcgc gccgacgaat
      721 ctccggcggc atcgaaaccg ctcgataatt aagatttagc ccgataccca aaatcacaaa
      781 ctcgatccgc tccgagtcgc acgtcgcttc gctcaaaaca ccggccaatt ttctaccatt
      841 gaccaaaata tcgttcggcc atttaatcac cggcgcttgc tcaattaaag gaacgatggt
      901 ttcagccagt gcaacggcag ccatgagcgt ggtttgcggc gcatgaacgg gaagaaagcg
      961 aggccgaaga atgacagaga agtacaggtt gacgcctggc ggcgattccc agcggcggcc
     1021 caggcgaccg cgaccgtgcg tttgcgcttc cgcaatgacg aattcgcctt cggctgcgcc
     1081 ctgttccgct agcattcgcg catggatgtt tgtcgacccg agctcggcga aataatgaaa
     1141 tttgccgccc aggcgttttg cttttagctg ttttgcgatt tggtcaatat cgaggggagt
     1201 caaggccggt ggagcagaga atcgattgtt ttaattcgtg ttcagggtga tcttcatatt
     1261 gaagtcgacc accggcgccg aatgcgtcag cgctccggag gagataaaat caactccggt
     1321 ttcggccact tgtcgaatgg tttcgagagt aatgccgccg gaagcctcga tccaggcccg
     1381 cttattcacc agctctacgg cttcggtcat ctgcgccagg gtcatgttat cgagcataat
     1441 cacgtcggct ccggcatcga gcgcttcccg aacctgttgc aaattgctgg cttctacttc
     1501 gactttggcc atgaatggag catggcggcg caccgctttg acagccttgg cgatggagcc
     1561 cgtcgcttct atgtgattat cctttaccag cgccgcgtca tagagcccga tgcgatgatt
     1621 catacctccg cccatgcgta cagcgtattt ttccaacgtc cggagtcccg gtgtggtctt
     1681 tcgcgtatcg agaactttgc agggataatt tttcaccgcc tcgacgtatt tgcgggtgag
     1741 cgtcgcattc ccggatagcc gctggagaaa attgagcgcc gtccgttcgg ccagcagcaa
     1801 cgcccgtacg ggcccaaccg cttcggcaac caccatgcct ggagtgacct ccgcgccatc
     1861 tttcgtgtgc aaacgcaatt ccacccggtg atcgatgaaa taaaaaatcc ggtcgagcaa
     1921 caccaacccc gccaccacac cctctttttt cgcgcgaaaa actccggtgc cgcgcgagtc
     1981 cgccgagatg gtcgccatgg tcgccaagtc gccggcgccg atatcctcgt ccaatgcgtc
     2041 gcggatcaat ctctcaattt gggggctgac atgaatatcc atccgcttaa ttcctttccg
     2101 cttggatttc ttgcaatttg tcgaggttcg ctctcaaagc gcgaatcttc tcttcaaacc
     2161 gagttgcctt ttcacgttcc ttatggacca cctcactctt ggccttcgcg atgaaatccg
     2221 cgttaccgag ttttttttgc acgcgcgcca gctcatcttc aattttatcc acttctttgg
     2281 acagccgcgc ccgctcctca ttgagatcga tcaaatcatc gagcggcaga tagatttctg
     2341 tggtgccgat caccgcagtg gccgctcctt tggggcgttc gctgccgtcg cggaattccg
     2401 attcgcttac ccgcgcaagc gagcggagat agggctcctg ctcgcgtaag aatcccaggt
     2461 cttccctcgc gccgcaaaag atcaccttga cttctttgct tggtggacaa ttcatttctg
     2521 tacggaggtt cctgatcgcc ctcaccgtg
//
LOCUS       DASULZ010000514         5111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_467946, whole genome shotgun sequence.
ACCESSION   DASULZ010000514 DASULZ010000000
VERSION     DASULZ010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5111
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467946"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VFY96_10550"
     CDS             complement(<1..335)
                     /locus_tag="VFY96_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606945.1"
                     /translation="MADQKTVDEVIKQIDREELAQLTKDLVDIPSPTGSERAIGEFIL
                     RWFSRHEIKSIGQEISPERINAIGIIEGKENGVSLTINGHMDTSYTGTEEDRMFCRAL
                     EPDSDLKGAV"
     gene            502..1053
                     /locus_tag="VFY96_10555"
     CDS             502..1053
                     /locus_tag="VFY96_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5606946.1"
                     /translation="MKRPLTLNVNGQQYDIEIEPNRLLLQVLRDHLGLTGTKEGCSIG
                     VCGACSVIVDGRLVSSCLTLAAGCNGKTIETIEGLAQDGKLHPLQQAFIEYGGFQCGI
                     CTPGQIMAAKALLDENSTPSEEEVKEWMSGNLCRCTGYYKILESVMAVVNGKTDPNYT
                     FRKRETTAKIQSLDVYKPVAKTN"
     gene            1238..3514
                     /locus_tag="VFY96_10560"
     CDS             1238..3514
                     /locus_tag="VFY96_10560"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5606947.1"
                     /translation="MSKTENYSVIGKRVQRVEGFDKVTGDSEYIADIYLPGMLVGKVL
                     RSPYPHARIRNIDTSKAEKLGGVRAVVTAEDTIKRPWGAFFADQYILSVGKTRYVGEE
                     VAAVAAIDPDIAEEAIDLIEVDWEPLPGVFDTEEAIKDGAPLVHDDKPNNIAMHLDLE
                     RGNVAEAFADSDVIVEDTFTSMPQWHCSIETIGSVAEYATSGKYTIYMNTQTLFNARY
                     RIATALGVPETDVRIIQSAVGGGFGGKSCDDNNALVAAVLAKKARKPVKLINTREEEF
                     LAGCRPRVFMKINVKIGFKKDGRIRAKALKVIADNGAYSAKAPAITGVAAMRHDTCYK
                     YSDVKIDAKLVYTNKVPTGAFRGFGNPSAEWAVEQAIDLGAHALGIDPMELARINAAE
                     VGYVSPHGNRVTSCELKQCLDRVEQMIGWKAKRANKQPNTGLGLACTVHVSGKRHFGD
                     YDGSSATIKINEDGKALILSGEGECGQGVHTAMCQIAAEELGIPVEDVEISRADTDLT
                     TFCLGAFASRLTYVSGNAVKNAASNVKQQLFEQAAEMLEANPQDLTSRDGKVFVKGAE
                     GKFLTISDVARARLFRHNGAPIVGSGSFDADSVLPDATRFGNESGAYNYGAQAAQVQV
                     DPETGQVKILQFVVASDCGTVIYPLGAEGQVEGGLAQGIGYALIEGLQIDEGRPINPN
                     FSDYRIPSMRDMPPLQHTFADSYEPTGPFGAKGLGELNMDPTAAVINNAIFDAVGVRV
                     KTLPITPEKILCALKEKG"
     gene            3588..4448
                     /locus_tag="VFY96_10565"
     CDS             3588..4448
                     /locus_tag="VFY96_10565"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX5606948.1"
                     /translation="MIRFDYLEPTTLKKAFSLLENYGDDARVIAGGTSLIIMMRQRLL
                     MPKIVISLARIPRFDRITYKPNDGLRIGAGARHRDIELSAVVKKHYPLLYETFRKVAQ
                     PRIRNMGTIGGNLAAGDPLTDPGASLIALDAEVTLTNSKGQRTLRLDEFFIDYYQTAL
                     EPGELLTQIHVPPPERPGWSHIKFTPRSIEDFATVGVAITLKTNNGTCEDVRLGLNSV
                     SSTIVHAKNAEAVLRGKAITDAVLREMGEVASTECEPTDDNRGSAEYKLDLIKVLVRR
                     AAEEALQRAS"
     gene            4480..4953
                     /locus_tag="VFY96_10570"
     CDS             4480..4953
                     /locus_tag="VFY96_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5606949.1"
                     /translation="MKRTIRVTVNGRVYQEDVEPRILLAHFLRENIGLTGTHIGCVVG
                     ECGACSVLLEGKLVKSCLHFAVQADGREVTTIEGLAKDGELNPVQDAFVKNYAFQCGY
                     CTPGMVMTSYALLQTNPNPTEAEIRKALAGNLCMCTGYVQIVDAVKEAAAMARNQ"
BASE COUNT         1244 a         1400 c         1375 g         1092 t
ORIGIN      
        1 accgcgcctt tgagatcgct gtcgggttcc agcgcgcggc agaacattcg atcttcttcg
       61 gtgccggtgt agctggtgtc catatggccg ttaatagtca acgagacacc gttttctttc
      121 ccttcgataa taccgatcgc attgatgcgc tcggggctga tttcctgacc gatgcttttg
      181 atctcgtgtc gtgagaacca gcgcaaaata aactcgccga tcgcccgctc ggaacctgtg
      241 ggactgggga tatcaaccaa gtctttggtc agctgagcga gctcctcgcg atcgatctgt
      301 ttgatgactt cgtctacagt cttttgatct gccattttct gatcctacaa cttcattgga
      361 ttttcggtgc actcccgagt acgtatcagg ttgccagcag atatcaatcg ggtaaacctc
      421 tttgcagcac caacaaaaat ctccgggaga atctcgagcg agaagttgaa tcttcattca
      481 gcaagggata ttttcaccgg catgaaacga ccgctaaccc tgaatgtaaa cgggcaacaa
      541 tacgacattg agatcgagcc gaatcgcctg ttactgcaag tactgcgcga tcatctcggg
      601 ctgactggca ccaaagaggg atgcagcatc ggcgtttgcg gcgcatgcag cgtgatcgtt
      661 gacggccgct tagtaagctc ttgtttgacc ttggctgctg ggtgtaacgg taagacgatt
      721 gaaacaatcg aaggattggc gcaagacggc aagctgcatc cgctccagca agcgtttatc
      781 gaatacggtg ggtttcaatg cggcatctgc acgccggggc aaatcatggc ggccaaggcg
      841 ctgctcgacg aaaattccac gcctagcgag gaagaagtca aagagtggat gtccggaaat
      901 ctttgccgct gcaccggcta ttacaagatc cttgagtcgg tcatggcggt ggtcaacggt
      961 aaaacggacc cgaattacac gtttcgcaag cgtgagacga ccgcgaaaat tcagagtttg
     1021 gacgtgtaca aacccgtggc gaaaacgaat tgacgcactt ataagtgagt caattctccc
     1081 tgagcgaagt gaagggcctc tcctggcact aatgatccga gaaacacaag aatctaagaa
     1141 agatttctcc cttccgtcga aatgacatgc gtcgtataac ggcgccttgc attcgatcag
     1201 tacacatttt gagaaccaat caacgagaac actgaccatg tctaaaacag aaaattactc
     1261 cgtcatcggc aagcgcgttc agcgcgtcga aggttttgac aaagtcaccg gcgattcgga
     1321 atacattgcc gacatctatc ttcctggcat gctcgtcgga aaagttttgc gaagtcccta
     1381 tccacacgca cgcatccgca atatcgatac gagcaaagcg gaaaagcttg gcggagtgcg
     1441 cgccgtcgtc accgccgagg acaccatcaa acggccttgg ggtgcattct tcgccgacca
     1501 atatattctc tcggtcggta agactcgcta tgtcggcgag gaagtcgccg ccgtggcagc
     1561 catcgatccg gacatcgccg aagaagcgat cgacctgatc gaagtcgatt gggagccgct
     1621 gccgggtgtg ttcgataccg aggaagcgat caaggacggc gcgcctttgg tgcacgacga
     1681 caaacccaac aatattgcga tgcatttgga tttggagcgc ggcaacgtgg ccgaggcttt
     1741 cgccgattcc gatgtgatcg tcgaagatac cttcacgagc atgccccagt ggcactgctc
     1801 catcgagacg atcggcagcg tcgcagaata cgccaccagc ggcaaatata cgatctacat
     1861 gaacacgcag acgttgttta acgcccgcta tcgcatcgcc accgcactcg gcgttccgga
     1921 aaccgacgtc cggattattc agagcgcggt cggcggcggc tttggcggca agtcgtgcga
     1981 cgacaacaac gctctggtcg ccgcagttct agcaaaaaaa gcccgtaaac ccgtcaagct
     2041 gatcaatact cgcgaagaag agtttctcgc cggctgccgc ccgcgcgttt tcatgaagat
     2101 caacgtcaaa ataggtttca aaaaagatgg ccgtatccgc gcgaaagcgc tgaaagtcat
     2161 cgccgacaac ggcgcctatt cggccaaagc gcccgcgatc accggcgtcg cggccatgcg
     2221 ccatgatacc tgttataaat attccgacgt caagattgac gccaaattgg tttacaccaa
     2281 taaggttcct accggtgcct ttcgtggttt cggcaatccg tcggccgaat gggcggtcga
     2341 acaggcgatc gatttaggcg cccatgctct cggtattgat ccgatggagc ttgcgcgcat
     2401 caacgccgcg gaagtcggtt acgtttcgcc gcacggcaat cgcgttacca gttgcgagct
     2461 taaacaatgc ctcgaccgcg tcgaacaaat gatcggctgg aaggccaaac gtgccaacaa
     2521 gcaaccgaat accggattgg gcttagcctg caccgttcac gtcagcggca agcgccattt
     2581 cggtgattat gacggcagct cggcgaccat caagatcaat gaagacggta aagccttgat
     2641 actgagcggc gaaggcgaat gcggacaggg cgttcacact gcgatgtgcc agatcgctgc
     2701 cgaagagctc ggcattcctg tcgaagacgt ggagatttcc cgtgccgata ccgatctcac
     2761 tactttttgt ctcggcgcct tcgccagccg gctcacttac gtgagcggca atgcggtgaa
     2821 gaacgcggcg agcaatgtga agcagcaact gttcgagcaa gccgccgaaa tgctcgaggc
     2881 caatccgcaa gatttgactt cgcgcgacgg caaagttttt gtcaaaggcg cagaaggaaa
     2941 gtttttaacc atttccgatg tggcgcgcgc cagactattt cgtcataatg gcgcgccgat
     3001 cgtaggttcc ggcagcttcg atgccgactc cgtgcttccg gatgccacac gcttcggcaa
     3061 tgaatcgggc gcttacaact acggcgcgca ggcggcgcaa gttcaggtcg atccggaaac
     3121 cgggcaagtc aagattctgc aattcgtcgt cgcctccgat tgcggtacgg tgatttatcc
     3181 cctcggcgcc gaaggacagg tggaaggtgg tctggcgcaa gggatcggct atgcgctgat
     3241 cgaaggattg cagattgatg aaggtcgacc cattaacccg aatttctccg actaccgcat
     3301 tccctcgatg cgcgatatgc cgccgctgca gcacacgttt gcagattctt acgagccaac
     3361 gggcccgttc ggggcgaagg gtttgggcga gctcaacatg gatcccaccg ctgcggtgat
     3421 caacaatgcg atcttcgatg ctgtcggcgt gcgcgtcaaa acgttaccga tcacgccgga
     3481 gaaaatcctt tgcgcactca aagaaaaagg ttagccgcca tgtgtcggtt tttattgctc
     3541 cacaatccaa aatcgaaaat ccaaaatcca aaatccaaaa taagatcatg atccgctttg
     3601 actacctaga accgacgacg ctgaagaaag ctttcagttt gctcgaaaat tacggcgatg
     3661 atgcgcgggt gatcgccggc ggaacatcct tgatcatcat gatgcgccag cgtctgctca
     3721 tgccaaaaat agtgatcagt ctcgcacgca tcccgaggtt cgaccgcatt acatacaagc
     3781 ccaacgacgg cttacgcatc ggcgcgggcg cgcgccatcg cgatatcgag ctctcggcgg
     3841 tcgttaaaaa gcattatccg cttttgtacg agacgtttcg caaggtggct cagccgcgca
     3901 tcagaaacat gggcaccatt ggcgggaatc tcgccgcagg cgatcctctg accgaccctg
     3961 gcgcaagttt gattgcgctc gatgctgaag ttacgctcac caacagtaaa ggccaacgaa
     4021 cgctccgtct ggatgaattt tttatcgatt actaccaaac ggcgctagag ccgggcgagc
     4081 ttttgacaca aatccacgta ccgccacctg aacggcccgg ttggtcacac atcaaattca
     4141 cgccgcgcag catcgaagac tttgccacag tcggtgtggc gattaccctg aaaaccaaca
     4201 acggtacctg cgaagatgtg cggcttggtc tcaattccgt ttcgtctacg attgttcacg
     4261 ccaagaacgc cgaagccgtt ctccgcggca aagcgatcac cgacgcggtg cttcgagaaa
     4321 tgggggaagt cgcttccacc gagtgcgagc ccaccgacga caatcgtggg tccgccgagt
     4381 acaagctgga tttaatcaaa gtcctggtcc ggcgcgccgc cgaggaagca ctccaacgcg
     4441 caagttgacc cgtctggcac cccaaggtat tcgtaaaata tgaagcgaac gattcgagtt
     4501 accgtcaacg gacgggtcta tcaggaggat gtcgagccga gaattctgct cgcgcatttt
     4561 ttgcgcgaga acattggtct aaccggcacg cacatcggct gcgtcgtcgg tgagtgcggc
     4621 gcctgctcgg tgctgctcga gggcaaactt gtcaaatcgt gcctacactt cgccgttcag
     4681 gcagacggtc gggaagtgac taccatcgaa gggttggcca aggacggcga acttaatcca
     4741 gttcaggacg ccttcgtcaa aaactatgca ttccagtgcg gttattgcac gccgggcatg
     4801 gtgatgacga gttatgcatt gttgcaaacc aacccgaatc caaccgaagc ggaaatccgc
     4861 aaggcgcttg ccggtaatct ctgcatgtgc acggggtatg tgcagattgt cgatgccgtc
     4921 aaggaagcgg cagcaatggc taggaatcag taaacgaaga tgtcgccaac tgaaactgaa
     4981 tttcaaaatc cccatctccc cctttttcaa acggcgattt ctttctctcc gcaattgaac
     5041 ctctctttga caaaaagggg caggggagat tttacacaaa tcatggcatt gagaaggtaa
     5101 gcattaatgc c
//
LOCUS       DASULZ010000515         4071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_975308, whole genome shotgun sequence.
ACCESSION   DASULZ010000515 DASULZ010000000
VERSION     DASULZ010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975308"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VFY96_10575"
     CDS             <1..690
                     /locus_tag="VFY96_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008163102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5606950.1"
                     /translation="GMRVHYGVVEAVKRIDLELRRGEITALLGSNGAGKSTTLLALSG
                     LVKPTQGSIRLNGVELGSLLPHQIVACGVVQAPEGREILATLTVKENLLLGAYRRRDR
                     RKIGADLDAMLVHFPRLRDRLHTAAGYLSGGEQQMLAIARALMAKPSLLLLDEPSMGL
                     APIVVTEIFEIVRALNQQGLTIFLIEQNARQALSIAHHGYVMENGQIVLSGTGKELLA
                     NPKVIEAYLGR"
     gene            944..1147
                     /locus_tag="VFY96_10580"
     CDS             944..1147
                     /locus_tag="VFY96_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008108598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5606951.1"
                     /translation="MDEILTAGEVAELLQIHPRTVYKLVKEGAIPGRKFGGGWRFSRS
                     EILAMVSPQGTTPPPRKMHTEER"
     gene            1311..2486
                     /locus_tag="VFY96_10585"
     CDS             1311..2486
                     /locus_tag="VFY96_10585"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5606952.1"
                     /translation="MAGALAGIKVIEAASYVTGPFASQLLADMGAEIVKIEEPKRGDP
                     FRGWGERNYAATFCSLNRNKRSVTLDLRADEGRNIAFKLASSADVLIQNFRPGVMEKR
                     GLGYDDLKQVNPKLVYCSISGFGPKGPYRDMPGYDTVGQARSGLLSLLTDPGKPQGMG
                     ISFSDHLTGMYACYGVLCALMNRMLTGKGQHVETSLLRASISFIGENAARYFETGHVP
                     RRKHRTTTAGVFAFEDCHGLPFVLHMSSPDKFWQNMFEVVGKPEWAADPRFNNRKARI
                     ENYDLLVESLAPIFRGGRRDEWLHRLIEKDVPAAPINSLDEVFVDPQVREYGFPIDVE
                     HPQMGKMKLIGNAVDMSRTPPAIERPPPTLGEHTEEILAELGYDNGAIAQLRQKQLI"
     gene            2512..3012
                     /locus_tag="VFY96_10590"
     CDS             2512..3012
                     /locus_tag="VFY96_10590"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5606953.1"
                     /translation="MLHDRYYSSVMSPRHPSLLALSHDHHHGLALALRCRKQALGQLK
                     PVGAVGLAERAQEFLAFYRSNLARHFEAEEAVLFPAMRKTVPVSAAIIDALVQDHQQI
                     RQALAQLELKTGLAKLIFDLGDLLERHIRKEERELFPLFEAQFETADAERIGRELKTV
                     LIVSPG"
     gene            complement(3019..3369)
                     /locus_tag="VFY96_10595"
     CDS             complement(3019..3369)
                     /locus_tag="VFY96_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606954.1"
                     /translation="MAKAKYLMIASMDVDPEHEAIFNEVYDREHVPNLSKVPGVLGIT
                     RYQRGTLTMNIGGEKKSIDIPNEPAYTAIYELESPEVLTSAAWDKAIEEGRWPSQVRP
                     HTKNRRHVLLKIME"
     gene            complement(3390..3752)
                     /locus_tag="VFY96_10600"
     CDS             complement(3390..3752)
                     /locus_tag="VFY96_10600"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5606955.1"
                     /translation="MILSKNSDVKGVVVKSIEGKPMYGGDLIVKPLIKGDEMTFLEIH
                     YTPGVGAPLHVHSHESLAYVVKGKVKMKVGGEEYILGPGDVCRHPKGVPHGVEGIEES
                     VVVEIKSPAPEISSFLGM"
     gene            complement(3814..>4071)
                     /locus_tag="VFY96_10605"
     CDS             complement(3814..>4071)
                     /locus_tag="VFY96_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606956.1"
                     /translation="NIDKSFSEDQNVHHSFTVSPETLVQACKIFKERKIPIDRLYYRA
                     KGFFTGCELYFFDPSGNRLELRDPTWHAGMPEPSFDELAQS"
BASE COUNT          925 a         1063 c         1102 g          981 t
ORIGIN      
        1 gggatgcgag ttcactacgg agtcgtcgaa gcggtgaaaa ggatcgatct ggaactccgg
       61 cgcggcgaaa tcacggcgtt gctaggcagc aatggagcgg gaaaatcgac aactctgttg
      121 gccttatccg gcctggtgaa gccgacgcaa ggctccatca gattgaacgg cgtcgagctt
      181 ggctctctct taccccacca gattgttgca tgcggcgtcg tacaagcgcc ggaaggccga
      241 gagattctcg ccacgctaac ggtcaaagaa aatctgctgt tgggcgccta ccggcggcgg
      301 gatcgacgga aaattggcgc ggacctcgac gcaatgcttg ttcactttcc aaggctaaga
      361 gatcggctgc ataccgcagc cggctatctc tcgggtggcg agcagcagat gttggctatc
      421 gcgcgggcgc tcatggcaaa acccagcttg ctgctcttgg acgagccttc catgggttta
      481 gcgccgattg ttgtcacaga gatttttgaa atcgtgcgcg ctctcaatca gcagggcttg
      541 acgatttttc tcatcgagca aaacgcccgt caggcgctga gcattgcgca ccatggttac
      601 gtcatggaga acgggcagat tgtgctgtcc ggaaccggca aagaactact tgccaatcca
      661 aaagttatcg aggcatattt gggtcgatag ttacgcatct ggatggccaa cggccaaact
      721 ggtcaactcg cgctaagcat aatcagttac tttgcattca tgttttgttt ctgtttgttc
      781 aatttttctc atttcctaca ctttaccgta ggattttcct gcaaactccc gtttgaaacc
      841 ctcattttgt cattttttgg ctaggttcgt cgctgttcgt ccaatggcat gaattctgcc
      901 ttagtctgta cgctgattat ttccaaatcg aggaggttag ctgatggacg aaatactcac
      961 cgcgggagag gtagccgagc ttctgcaaat tcatccgcgc acagtctaca agctcgtaaa
     1021 agaaggtgcc attcccggac gtaagtttgg tggcggatgg cgctttagcc gtagcgagat
     1081 tcttgccatg gtatcacccc agggaaccac accgccgccg cgtaaaatgc atactgaaga
     1141 acgctagctt tcttctaatt cgattccaaa ttgttatggc acgccggtcg gagctgtcgc
     1201 ccggcgtgcc accaattcag cgacagaccg tccggttcaa aatcccaagc tctcttgact
     1261 gccaagcacc gcaacgctag gacatacgga ccattctttc acggtggcaa atggccggtg
     1321 cacttgcggg aattaaagta atcgaagcgg caagctatgt tacgggtcct tttgcgtctc
     1381 agttgttggc cgacatggga gcagaaattg ttaagatcga agagccaaag cgcggtgacc
     1441 cgtttcgtgg ttggggagaa agaaattacg ccgcaacatt ctgcagttta aatcgcaata
     1501 agcggagcgt cacgctcgat ttgcgcgccg acgagggccg gaacatcgcg tttaaactcg
     1561 cctccagtgc ggatgtgctg atccagaatt tccgtcccgg cgttatggaa aagcgcggcc
     1621 ttggctatga cgacctgaag caggtaaatc caaaactggt ttattgctca atttccggct
     1681 ttggcccaaa ggggccttat cgggatatgc ccggctacga taccgtgggc caggcccgga
     1741 gcgggttact gagtcttctg accgatccgg gaaaaccgca aggaatggga atctctttct
     1801 cggatcattt aacgggaatg tacgcatgtt atggagtctt gtgtgcgctg atgaaccgga
     1861 tgcttaccgg gaaagggcag cacgttgaaa cttcgctgct gcgcgcttcg atatccttta
     1921 tcggtgaaaa cgccgcacgc tatttcgaga ccgggcatgt cccgaggcgc aagcaccgga
     1981 ccaccacagc cggggttttt gctttcgaag attgccatgg tttgccgttc gtcctgcata
     2041 tgtcgtcacc ggacaagttc tggcaaaaca tgtttgaggt agttggaaaa cctgaatggg
     2101 cggcagatcc acgtttcaac aaccgcaaag cgcggatcga aaattacgat ctgctggttg
     2161 aaagccttgc cccgatcttc cgaggcgggc ggcgcgacga gtggttgcac cggctcatcg
     2221 agaaagatgt tccagcagcg ccgatcaaca gcttggacga agtattcgtc gacccgcaag
     2281 tgcgcgagta cggtttcccg attgacgtcg aacatccaca aatgggaaaa atgaagttga
     2341 tcggcaatgc cgtcgacatg agtcggacgc ctccagccat cgagcgacca cctcccacgc
     2401 tgggcgagca taccgaggaa attctcgccg aacttggata cgataacggc gccatcgcgc
     2461 agctccgcca aaagcaactc atctagaaaa gtggagggat tgagtggcgg agtgcttcat
     2521 gatcgctact actcctctgt catgtctcct cgtcatccca gcttgttagc gctatcccat
     2581 gaccatcatc acggcctggc attagcgctt cgctgtcgca aacaggcgct tgggcagctc
     2641 aaaccggtgg gcgcagtagg tctggccgag cgggcccagg aatttttggc cttctataga
     2701 agcaatctcg cgcgtcattt cgaggcagaa gaagcagtcc tgtttcctgc gatgcgcaaa
     2761 acggtgccgg tgagcgcggc gatcatcgat gcgctcgtgc aggatcatca acagatccgt
     2821 caagcgctgg cgcagcttga attaaaaacg ggattggcca aattaatttt cgatctcggt
     2881 gatctgctcg agcgccacat ccgcaaggaa gagcgcgagc tgtttccttt atttgaagca
     2941 cagtttgaaa ctgccgacgc cgagaggatc gggagagagt taaagacggt tttaatcgtg
     3001 agcccaggat gacgcgaact attccataat tttcagtaaa acgtgccggc gatttttggt
     3061 gtgcgggcgc acttggctcg gccaacggcc ttcttcaatg gctttatccc atgccgcgct
     3121 ggttagtact tccggactct ccaattcgta gattgcggtg tatgcgggct cgttcggtat
     3181 gtcgatggat tttttctcac caccgatgtt catggtcaga gtcccgcgtt gatagcgggt
     3241 gatgccgaga acgcccggca ctttgctcag attgggcacg tgctctcggt cgtagacctc
     3301 gttaaaaatc gcctcgtgct cgggatcgac gtccatgctg gcgatcatta aatattttgc
     3361 ttttgccata ggaatctccg cgtgcattgt cacatgccca gaaagcttga aatctccggc
     3421 gctggggact tgatttcgac cacgaccgac tcttcgatgc cttccacgcc atgaggcacg
     3481 cctttcgggt gccggcaaac atcgcctggg ccgagaatat attcttcgcc gcccactttc
     3541 attttgactt ttcctttcac gacgtaagcc aaagactcat ggctgtgcac gtgcaagggc
     3601 gctcccacac cgggtgtata atgaatttcc agaaaggtca tttcatcccc tttgatcagc
     3661 ggtttcacga tgagatcgcc gccgtacata ggcttgcctt cgatggattt tacaacgacg
     3721 cctttgacgt ctgaattctt agacaaaatc atgaatgact ccttgttaga actacatgcg
     3781 caacgggtaa tcgcccagaa cagcgccgtg tgattacgat tgcgcgagtt catcgaaact
     3841 cggctccggc atgccggcgt gccaagtcgg atcgcgcaat tccaagcgat taccgctcgg
     3901 gtcaaagaaa taaagctcgc agccggtaaa aaaccctttg gctcggtagt aaagccgatc
     3961 gatgggaatt tttcgttctt tgaaaatctt gcatgcttgc acgagggttt cggggcttac
     4021 ggtaaatgaa tggtgcacat tctgatcctc gctaaatgat ttgtcgatgt t
//
LOCUS       DASULZ010000516         2601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_874110, whole genome shotgun sequence.
ACCESSION   DASULZ010000516 DASULZ010000000
VERSION     DASULZ010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874110"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..1070
                     /locus_tag="VFY96_10610"
     CDS             48..1070
                     /locus_tag="VFY96_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606957.1"
                     /translation="MAHLYVATNGLSVWSSADLGETLLRMSTGTGLYSGSQVWALALH
                     PSLPHVLFAGTNSGIYRLDQEEENWTHIPSPADDAMIVTALACAPDNPDVIIAGTQPA
                     ALYRSENGGRSWEKISVPMKPYAVTGYYLGDKPYPEGHPGAYGRKHWTRVTQIVFDPK
                     DSSLVWAGVEIDGAWRSTDGGKRWEKSDRGIETQDIHGFAVMHNGGRVLFATTNAGLH
                     VSRDNGTSWSMRPIESKWQYTRSIVERPDGSGVMFMTNGDGPPGTAGRLFRSRNHGAD
                     WEDANLPGEVESSAYFLAANPADPKLIYAAATLGQIYRSTDGGESWTALKRRLGEIRA
                     LAWLPN"
     gene            1413..2402
                     /locus_tag="VFY96_10615"
     CDS             1413..2402
                     /locus_tag="VFY96_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEX5606958.1"
                     /translation="MAEKWHIKKAGPGLAIEEMIARLLGSEFVVEEFDPTRPLAQQVD
                     NSNVLLVRSMPVTPEVIDAAPRLRLIQRPGVHLEGVDVEYAAIKKIPVCNVPASLTHG
                     GNDVAEHVMFLALALAKRYREALASLGTRRVGTPSTHVLRGKVFGIVGFGRTGSAVVQ
                     MARGFGMRVWAVKRTVSEGMGDAMGLEWLGRERQLSELLRESDFVSLHVPLNKETEGL
                     IGAAEITLMKPSAFLINVARGRVVDRAALTAALSERRLAGAGLDVFWDEPIDPDDPLL
                     ALPNVIATPHVANMTMETIETIAQAAADNIRRVYAGLPPLHEILKSPHVASKS"
BASE COUNT          546 a          735 c          810 g          510 t
ORIGIN      
        1 ttataaaaga ctagcggcgc gcagctccgc taacaaaagg aggcgagatg gcacatttgt
       61 acgtggcgac caacgggctc tcggtttgga gcagtgcgga tctcggcgaa acgctgctgc
      121 gcatgtcgac tgggaccggc ttgtacagcg gcagtcaggt atgggcgctg gcgcttcatc
      181 cttctttacc gcacgtgctt ttcgctggca cgaattccgg catctaccgg ttggatcagg
      241 aggaagagaa ttggacccat ataccgtcgc cagcggatga cgcgatgatc gtcactgcgc
      301 tcgcttgcgc cccggataac ccggacgtca ttatagcggg aacccagccg gcggcactct
      361 accgttccga gaacggcggc agaagctggg aaaaaatcag cgtgccgatg aagccgtacg
      421 cggtgaccgg atattatctg ggcgacaagc catacccaga gggacatccc ggcgcctacg
      481 gccgcaagca ctggacaagg gtgacgcaga ttgtgttcga ccccaaggat tcttcgctcg
      541 tctgggctgg agtggagatc gacggcgcat ggcgcagtac cgacggcggt aaacgatggg
      601 agaaaagcga tcgtgggata gagacacaag acattcacgg ctttgccgta atgcataacg
      661 gcgggcgcgt tcttttcgcg acgaccaacg ccggcttgca tgtgagccgc gataacggca
      721 cgagctggag catgcggcca atcgaatcaa agtggcaata cacccgctcg attgtcgagc
      781 ggccggacgg aagcggcgtc atgttcatga cgaacggcga cggcccgccc ggcacggccg
      841 gccgcctatt tcgcagccgc aatcacggcg cagactggga ggacgcgaac ctgcccgggg
      901 aggtcgaaag ctcggcatat tttcttgccg ccaatccggc ggatccaaag ctgatctatg
      961 ccgccgcgac tctcgggcag atctatcgca gcaccgacgg cggcgagagc tggaccgcgc
     1021 tcaaacgccg gctcggcgaa atccgcgcat tggcgtggct tccgaactga ccgtcctcgc
     1081 gagcgccaga gtttgtgaaa actcgcccgt cgtccttcga caagctgcta tgtggatgtc
     1141 aagtcacaga cacacgagtc gacgccacag aaccctaaac cgctcatggt gagctcgtcg
     1201 aaccatgttc agactttcgc acagtctggc gaatgcaagg atcgaagaaa gatttctctc
     1261 ttcgttcgaa atgacaaact gccatgtttg tggtttgcgc ctcacgatat atttttcggt
     1321 agatggttcc gtaggggcgc agcatgctgc gccctctggt ttctccgccg cgcgatttgt
     1381 ttcgtgcaat agaatttctg gcaataagtc ggatggctga aaaatggcac attaaaaaag
     1441 ccggtcctgg tctcgcaatc gaagagatga tcgcgcggtt gctcggttcg gaatttgtgg
     1501 tcgaggagtt cgatccgaca cggccgctgg cgcaacaagt cgacaactcg aacgtgctgc
     1561 tggtccgcag catgccggta accccggaag tgatcgatgc cgcgccccgc ctgcgtttga
     1621 ttcagcggcc gggggtgcat ctggagggag tcgatgtcga atacgccgct ataaaaaaga
     1681 ttcccgtgtg caatgttccg gcatcgctca cccatggcgg caatgacgtc gcggagcacg
     1741 tcatgtttct tgcgctggcc ctggccaagc gatatcgcga agcgctggcc tcactcggca
     1801 cgcgccgcgt tggcacgccg agcacccatg tgctccgcgg aaaagtcttc ggcatcgtcg
     1861 gcttcgggcg aacgggaagc gccgtggttc agatggcgcg cggcttcggc atgcgcgtct
     1921 gggcggtgaa acgcactgta agcgagggaa tgggcgacgc gatgggactc gagtggctgg
     1981 gaagggagcg ccagctttcg gagcttcttc gggagagcga ttttgtttca cttcacgtgc
     2041 cgttgaacaa agaaaccgaa gggctcatcg gagcggcgga aatcacgcta atgaagccga
     2101 gcgcttttct gattaacgtc gcgcgcggcc gcgtcgtcga ccgcgcggcg ttaacggccg
     2161 cgctcagcga acggcggctt gcgggagccg ggctcgatgt cttctgggac gagccgatcg
     2221 acccggatga tccactgctt gcgcttccaa acgtcatcgc cacgccgcac gtggcgaaca
     2281 tgacgatgga gacgatcgag acgatcgccc aggcagcggc ggacaacatc cgccgagtgt
     2341 acgcgggatt gccgcctttg catgaaattc tgaaatctcc gcacgtggcg agcaaatcct
     2401 aattattttg tgaacgatgg gtgcctcggc cgtggccgct tgcagccggc gagtccgttc
     2461 accgcgcgcg tacctgaaaa tcgccctggt gggcgatgag tgaatcgaaa aattttcggc
     2521 cgttccgggc tgtcagttgt ctcatcgcgt ttcagaagat ttggcaatct gctgctagat
     2581 cacgtaaaat cgcagcaatt t
//
LOCUS       DASULZ010000517         5550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_772056, whole genome shotgun sequence.
ACCESSION   DASULZ010000517 DASULZ010000000
VERSION     DASULZ010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5550
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772056"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VFY96_10620"
     CDS             <1..597
                     /locus_tag="VFY96_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX5606959.1"
                     /translation="KAIADAKEVNMPADNIKRAIQKGTGELEGGQLEELQYEGYGPGG
                     VAIIAQVLTDNKNRTVSEIRKLFSKHGGNLGESGSVSWMFDKKGLITVEKSQVDEDRL
                     MDVVLDAGAEDVRDEGDIFEVVTQPEDFEKVKDRLDHEKIAVGSAQLTLLPKTTVDVD
                     AKNVEQVLKLSEELEEHDDVQGVAANFNIPTELMDKAS"
     gene            610..1191
                     /gene="ruvC"
                     /locus_tag="VFY96_10625"
     CDS             610..1191
                     /gene="ruvC"
                     /locus_tag="VFY96_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011637638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX5606960.1"
                     /translation="MPKSSAKPLHREIILGIDPGSRITGWGLIELIGTELHHLAHGTI
                     ATNAAMALPSRLSQIHTGLRGIIERHQPATVSLEKVFFSRNVQSALKLGQARGVALLA
                     AAEREVSISEYSAAEIKLAVVGYGRATKEQVQRMVNALLCLRGAIRADAADALASAIC
                     HAHRRSFQDLVVRSYGKVGSTSWRDYLETASER"
     gene            1303..1941
                     /gene="ruvA"
                     /locus_tag="VFY96_10630"
     CDS             1303..1941
                     /gene="ruvA"
                     /locus_tag="VFY96_10630"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX5606961.1"
                     /translation="MPRFFAALRSDSFTDVIAQISGALAQKIPGEAIVDVGGVGYQIF
                     IPLNVFYRLPEIGAPVKLQIHTHVREDALQLFGFNDFAEKQVFLLLIGVSGIGPKLAV
                     NILSGIPAEELARALRESDQARLLSIPGVGRTLAERMIVELKDKLTTLAPAPVESADR
                     EPGSQLLLDAVSALVNLGYKRVDAEKAVRDVLKRGTHSLEGVLKESLRLLSP"
     gene            1967..3001
                     /gene="ruvB"
                     /locus_tag="VFY96_10635"
     CDS             1967..3001
                     /gene="ruvB"
                     /locus_tag="VFY96_10635"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX5606962.1"
                     /translation="MTAMLSGTKLDEDLSFDVTLRPRGFDEYVGQDAVKENLKVAIAA
                     AQARRDVLDHILFHGPPGLGKTSLAYIIAREMGVNIKATSGPVVERPGDLAALLTNLD
                     DGDILFIDEIHRLNHIVEEVLYPAMEDYQIDIMVGQGPAARSIKLPLKRFTLIGATTR
                     SGLLTSPLRDRFGHIHRLDFYDTAALGKILQRSAAILSVREEADGIMEIAARSRGTPR
                     IANRLLRRVRDYTQVKGDGTITKRLTEQALSMLEVDRLGLDKMDTMLLLTLIDKFAGG
                     PVGVETLAAAIGEEKDTIEDVYEPFLIQAGFIQRTPRGRVATMRAYDHFGRPQKEGAT
                     NLTRKQPTLF"
     gene            3044..3256
                     /locus_tag="VFY96_10640"
     CDS             3044..3256
                     /locus_tag="VFY96_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HEX5606963.1"
                     /translation="MSGLGKTLILIGVVLVVVGAVFSLAGKLPWLGRLPGDITIQREH
                     FTFYFPLTTCILISVVISLVLYFFRR"
     gene            3295..5529
                     /locus_tag="VFY96_10645"
     CDS             3295..5529
                     /locus_tag="VFY96_10645"
                     /inference="COORDINATES: protein motif:HMM:NF039586.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5606964.1"
                     /translation="MEAVNPAAAVAQSLKFEENKRRKREGIVILVTALMVAALIFFEV
                     QLPDVTPEYSLGGNVVFFLLININIILLGLLVFLVVRSLIKLVVEHKRRMLGSRLQAR
                     LVLAFVALSIVPSVLLFIIAGGLLRRSFDRWFDSKVESALQGSLEIGQTYYQNSANNA
                     IFYARQLSQRITEEGLVNPSRVAELKRFVESKQLEYNLGTVELFLPDGQPFVVAFNQQ
                     VPTGVTLKPESEFLNRALRGLEVTRTEAFGDGDVIRGGVPVYARDKRIMGVVVVDYYV
                     PKSISKRAIQISQSYEQYKFLTFLKAPVKNSYLLTMLLITLVIIFAAIWCGIYLSKGI
                     IVPIQKLAEGTHQVAHGNWDYKIEAGGDDELGVLMNSFNHMTADLKQIKLELERRGTV
                     VQTLLANIAAGVVSVDPAGRITTWNKAAERILAVPAERALGRYYHDVFRAEPLRVIRE
                     IVDSVKSGESIEQEIKLSLQDQLRNVMACGATLHDDDGSILGVMLFLEDITQIQKVQR
                     MEAWREVARRIAHEIKNPLTPIQLSAERLRKRYGRVLVEDGAILDKCTATIIQQVEEL
                     KKLVNEFSQFARLPSARLMASDLNEIINDSLVLFKEGHAGIQFQFRPGMIPELELDRE
                     QIKRVLMNLLDNAVAAVQGRGAVTLATNFDPESGVVTFEVGDNGCGIAPEMRTKVFEP
                     YFSTKENGTGLGLTIVSQIVEDHRGYIRVRPNDPQGTRFIVELPAGIRARVHVTPKNA
                     LHSL"
BASE COUNT         1210 a         1494 c         1639 g         1207 t
ORIGIN      
        1 aaggccatcg cggacgcgaa agaagtgaac atgcccgcgg acaacatcaa gcgcgcgatc
       61 caaaaaggca ccggagagct tgaaggcggg caactcgaag agctccagta cgaaggttat
      121 ggccccggcg gcgttgcgat cattgcgcaa gtcttgaccg acaacaaaaa ccgcacggtt
      181 tccgaaatcc gcaagctttt cagcaaacac ggcggcaatt tgggcgagag cggctcggtg
      241 tcatggatgt tcgacaagaa gggtctgatc accgtcgaaa aatcgcaggt ggatgaagat
      301 cgtttgatgg acgtggtgct cgatgccggg gccgaagacg tccgcgacga gggtgatatt
      361 tttgaagtgg tgacccagcc ggaagacttc gagaaggtca aggatcgtct cgatcacgag
      421 aagatcgcgg tcggcagcgc gcaattgacg ctgctgccaa aaaccacggt cgacgtggac
      481 gccaaaaacg tcgagcaggt tttaaaactg agcgaagagc tcgaagagca tgacgacgtg
      541 cagggcgtcg cagctaactt caacattcct accgaattga tggacaaggc aagctaagcc
      601 gtggcgccga tgccgaagag cagcgccaag ccgctccatc gtgaaatcat tctcggcatt
      661 gatcccggct cacggatcac gggctggggg ttaattgaac tgatcggcac tgagctgcat
      721 catcttgccc acggcacgat cgcgacgaac gcggccatgg ctctaccttc gcgcttgagc
      781 cagatccaca ccggtttacg agggattatc gagcgccatc agcccgccac cgtaagcttg
      841 gagaaagttt ttttttcgcg gaacgtccaa agcgcactta aactgggtca agccaggggg
      901 gttgccttgc tcgcggcagc ggagcgtgaa gtgagcatca gcgagtactc tgccgcggaa
      961 atcaagctgg ccgtggtggg ctacggccgc gccaccaagg agcaggtgca aagaatggtg
     1021 aacgcgctgc tctgtttgcg cggcgcaatt cgcgccgatg ctgccgacgc gctggcgagc
     1081 gctatctgcc atgcacatcg gcggagtttt caagatttgg tcgtccgctc ctacggtaag
     1141 gtggggtcca ccagctggcg cgactacctc gaaacggctt cagagcgtta gccgatcgcg
     1201 agattcatat gctcttttgc gcttcaacgc cttttccgaa ccgtcctcaa atacgccttt
     1261 gctcgtgcct gcatcgagcg ccttccgtct caattttggc agatgccaag attttttgcc
     1321 gcgcttcgtt ccgactcgtt tactgacgtg atcgcgcaaa tcagcggggc actggcccaa
     1381 aaaattccag gcgaagccat cgttgatgtc ggcggcgtgg gataccagat tttcattccg
     1441 ctgaatgttt tttatcgact gccggaaatt ggcgcaccgg tcaaattgca gattcacact
     1501 cacgtacgcg aggacgcgtt gcagcttttc ggctttaacg attttgctga aaagcaagtt
     1561 ttcttactgc tcataggcgt ctcgggaatc gggcccaagc tcgcggtcaa tatactttcc
     1621 ggtattcctg ccgaagagct ggcgcgtgcg ctgcgcgaga gtgaccaggc gcgtttgctc
     1681 agcatccccg gcgtaggccg tacgctcgcc gaacgcatga tcgtcgaact caaggacaag
     1741 ctaacgacgc tggctccggc gccggtcgag agcgccgacc gagaacctgg ttcgcagctc
     1801 ttgttggacg cggtttcggc gctggtcaat ctgggttata agagagttga cgccgaaaaa
     1861 gcggtgcggg atgttctgaa gcggggcacg cattctcttg aaggcgtatt gaaagaaagt
     1921 ttgcggcttt tgagtccgtg atcgcgaatg ggttgcgtga ctgattatga cagccatgct
     1981 gagtggcaca aaactcgatg aggatctaag cttcgacgtt accctgcgcc cgcgcggctt
     2041 cgacgagtac gtcggccagg acgccgtcaa agaaaacctc aaggtcgcga tcgccgcggc
     2101 tcaagcgcgt cgcgacgttc tggatcatat tctttttcac ggcccccccg ggctgggcaa
     2161 aacctctctt gcgtacatca tcgcgcgcga aatgggcgtc aatatcaagg cgacctcggg
     2221 acctgtggtg gagcggcccg gtgatctcgc tgccctgctc acgaatctcg acgatgggga
     2281 tatcctgttc atcgacgaaa ttcatcggtt gaaccatatc gtcgaagagg tgctgtatcc
     2341 ggcgatggag gattatcaga tcgatataat ggtcggccag ggaccagcgg cacgctcgat
     2401 caagttgccg ctcaagcgct ttaccctgat cggcgcgacc actcgctcag gcctcttgac
     2461 atcgccgctg cgtgatcgtt tcggtcatat ccaccgtctc gatttctacg acacggccgc
     2521 gctagggaaa atcctccagc gctcggcggc gattctcagc gtgcgcgaag aagccgacgg
     2581 catcatggag atcgcggcac gctcacgcgg cacgccgcgc atcgccaacc gcctgctccg
     2641 gcgagtgcgc gactacactc aggtgaaagg cgacggcacg atcaccaagc ggctcaccga
     2701 gcaagcgctc agcatgctgg aggttgaccg gctagggctg gataagatgg acacgatgct
     2761 gctgcttacg ctcatcgaca agtttgccgg cggcccggtc ggcgtggaaa ctctggccgc
     2821 agcgatcggc gaagagaagg acacgatcga agatgtttac gagccgtttt tgattcaggc
     2881 cggtttcatt caacgcaccc cgcgcggacg cgtcgcaacg atgcgggctt atgatcattt
     2941 cgggcggccg caaaaggaag gggcgacaaa cctgacgcga aagcagccca ccttgtttta
     3001 gtcagccggc gtttgagaat gaaaccgaat ggagtgatca cccatgtccg gattgggtaa
     3061 gacattgatc ctgatcggcg tcgtgctggt cgtcgtcggc gcggtgtttt ctcttgcggg
     3121 caaactgccc tggttggggc gtctccctgg cgacataacg atccagcggg aacacttcac
     3181 tttttatttt ccgctgacga cctgcattct catcagcgtc gttatttccc ttgttcttta
     3241 tttttttaga aggtaaaatt ttctgctgtc tcaccggtcc acaatctgcc ctctatggaa
     3301 gccgtaaatc ccgctgccgc cgtcgcgcag agcctgaaat tcgaagagaa caaacggcgc
     3361 aagcgcgaag gcatcgtcat tctggtcacg gcgctgatgg ttgccgccct gatctttttt
     3421 gaagtgcagt tgcccgatgt gacgcccgaa tactcgctcg gcggcaatgt cgttttcttt
     3481 ctcctgatca acatcaacat tatcctgctc ggcttgctgg tttttttggt cgtgcgcagc
     3541 ttgatcaagc tcgtcgtcga gcacaaacgg cgcatgctcg gctcgcgatt gcaggcgcgc
     3601 ctcgtgcttg ccttcgtcgc attgtcgatc gttccgagcg tgcttttgtt catcattgcc
     3661 ggcggcttgt tacggcgctc gtttgaccgc tggttcgatt ccaaagtcga aagcgcgttg
     3721 caaggttctt tggaaatcgg ccaaacctat taccaaaact ccgccaacaa tgcgattttc
     3781 tatgcgcggc aactaagcca gagaatcacc gaagagggac tggtcaaccc ttctcgagtc
     3841 gcagagttga agcgatttgt cgaaagtaag cagcttgagt acaacctcgg caccgtggag
     3901 ctttttttgc cggatggtca gcccttcgtc gttgccttca accaacaggt gccgacgggg
     3961 gtcacgctca aacccgaaag tgaattttta aatcgcgcgc tgcgcgggct ggaagtgacg
     4021 cgcacggagg ccttcggcga tggcgatgtg attcgcggcg gcgtgccggt gtatgcgcgc
     4081 gacaagcgga tcatgggtgt ggtggtggtg gattactatg tgccgaagag tatctccaaa
     4141 cgagcgatcc agatttccca gtcctacgaa cagtacaagt ttttaacttt tctcaaggcg
     4201 ccggtaaaga acagttatct tctcaccatg cttttgatca cgctggtgat catttttgcc
     4261 gcgatctggt gcggcattta tttatccaag ggcattatcg tgccgatcca gaaacttgcc
     4321 gagggaactc atcaggtggc gcacggcaac tgggactata agatcgaagc cgggggagac
     4381 gatgagctcg gcgtactgat gaactcgttc aatcacatga ccgccgatct gaagcaaatc
     4441 aagctggagc tcgagcggcg cggtacggtg gtgcaaaccc tgcttgccaa catcgcggcg
     4501 ggcgtcgtct cggtggatcc ggctgggaga atcaccacct ggaacaaggc tgcggaacga
     4561 attttggctg tgccggcgga gcgcgcgctg ggcagatact accacgacgt gtttcgcgcc
     4621 gaaccgctgc gtgtcattcg tgagatcgtc gacagcgtca agagcggtga gagcatcgaa
     4681 caggaaatca agctgtcgct tcaggatcag ttacgaaacg tcatggcatg cggcgcgacg
     4741 ctgcacgatg acgatggctc gatcctgggc gttatgctgt ttctggaaga tattacccag
     4801 attcaaaaag tgcaaagaat ggaagcatgg cgcgaagtcg cgcgccggat cgcgcatgaa
     4861 atcaagaatc cgctgacgcc gatccagctt tccgcagaga gattgcgcaa acgttacggc
     4921 cgggttttgg tcgaagacgg ggcgatcctc gacaaatgca ccgccaccat cattcagcag
     4981 gtggaggagc ttaaaaagct cgtgaacgag ttttctcagt tcgcccgctt gccgtccgct
     5041 cggctcatgg ccagcgattt gaacgagatc atcaatgatt cgctggtgct attcaaagag
     5101 ggtcatgccg gcattcaatt tcaatttcga ccggggatga ttcccgaatt ggaattggat
     5161 cgtgagcaaa tcaagcgcgt cttaatgaat ttgctggata atgcggtggc ggcggtgcag
     5221 ggacggggag cagtcacgct cgcgacgaac tttgatcccg agagcggtgt ggtcacgttc
     5281 gaggtcggcg ataatggatg cggcatcgca ccggaaatga ggaccaaggt cttcgagccg
     5341 tatttttcca cgaaggaaaa cggcacgggt cttggcctaa cgattgtgag ccagattgtc
     5401 gaggatcatc gtggctacat ccgggtgcgg ccgaacgatc ctcagggaac gcggtttatt
     5461 gtcgaattgc cggcggggat acgcgcgcga gttcacgtta cgccgaaaaa cgcgctacac
     5521 tcattataat cggaggagcg gtgcaggaac
//
LOCUS       DASULZ010000518         6358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_468082, whole genome shotgun sequence.
ACCESSION   DASULZ010000518 DASULZ010000000
VERSION     DASULZ010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6358
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468082"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VFY96_10650"
     CDS             complement(<1..366)
                     /locus_tag="VFY96_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX5606965.1"
                     /translation="MFKRILIANRGEIAVRIARACRELDIESVAVYSEADRESLHVKY
                     ADYAYLIGPAPSIQSYLAIDRIIDVCKRSGAEAVHPGYGFLAENPAFAQRCKDEGIVF
                     IGPSPKVIDQMGDKVKAREI"
     gene            complement(405..1955)
                     /locus_tag="VFY96_10655"
     CDS             complement(405..1955)
                     /locus_tag="VFY96_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX5606966.1"
                     /translation="MVTINNLDKLRELNERAEAGGGAERARRQHEQGKLLARERLNIL
                     LDSGSFVELDRLRTHECTDFGMEQQKFFGDAVVTGYGAIDSRLVYVYSQDFTVFGGSL
                     SRVVADKICKVMDMALKNGAPIIGINDSGGARIQEGVDSLAGYGEIFQRNVMASGVVP
                     QITAILGPCAGGAVYSPAITDFIFMLKTNSYMFITGPDVIKTVTHEEVSKETLGGAET
                     HAGRSGVAHFAAGSEKDCLLMIRDLIGFLPGNNQEDPPFRPNTDDPLRRDKRLRTLIP
                     DNPNRPYDMKELITAVVDEGYFYEVQAEFARNILVGFAHLGGRSVGIVANQPANLAGC
                     LDINAAVKAARFIRFCDCFNIPLVTFVDVPGFLPGTEQEYHGIIRHGAKLLYAYAEAT
                     VPKVTVVTRKAYGGGYIVMASKHLRGDLNFAYPTAEIAVMGPEGAINIVFRDELQKAA
                     DPANAREQLIGKYRETFANPFKAAESGYIDGIIYPEETRPTLIRALDMLKNKQDHHPP
                     KKHGNIPL"
     gene            2140..3336
                     /locus_tag="VFY96_10660"
     CDS             2140..3336
                     /locus_tag="VFY96_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX5606967.1"
                     /translation="MFETQTARQKKSKLAGDEREAVKLRGDQAILLVAASEGDANMLY
                     AVGFFVPDPFIFFQHKEKKFAVLSDLEIDRAKKQAHVHRVLSLSLYQRKLRQLGKPAA
                     MIDVLDLIFRERGIRSLIVPANFSALLSDQLRAKGYSVQIKRDPFWPERETKNRVEVA
                     QITESLRIARLGLEAGIRMLKRTRNKRDGYLYLNGRRLTSEMLKTAVNTTIMAQGWLP
                     SHTIISSGNQCVDPHHEGSGPIKADTSIIFDIFPRSQHNGYFGDLSRTVVRGRASDKL
                     KEIYATVQAGQQIGYRQIRDGVNGKEVHQNILALFEARGFHSGRINGRMQGFFHGTGH
                     GLGLDIHEPPRIAPVDAILRAGHVVTVEPGLYYLGIGGVRLEDVVVVTAKGNRNLTDC
                     PQFLEI"
     gene            3399..4118
                     /locus_tag="VFY96_10665"
     CDS             3399..4118
                     /locus_tag="VFY96_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606968.1"
                     /translation="MNDSEIAELIAKLAHHDKPTLRAAVDALILLATEFSTVRETLIR
                     RLPEAEPSKKWPIAYILAHLPQPGQPVIRSLLDALDHRDADIRWAIALLLVRVAKLEP
                     DRLNELIDLCATGTANQKRMALYCIRDLTLSDPQSLAALLRALVDVDSTVRVAAAIAL
                     QSRSDLDRAGRNFLLQTYLNDAELKVRNTAAIALANLGETSSEFLCALQEAVLGADAQ
                     VRKTAMKALDLIKKRSASAEN"
     gene            complement(4444..5262)
                     /locus_tag="VFY96_10670"
     CDS             complement(4444..5262)
                     /locus_tag="VFY96_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5606969.1"
                     /translation="MTISYETLLVTKENGITTIAFNRPEKRNAMSPQLHREMFELLTD
                     LRYDSETRVIVLTGVGENFCAGQDLKQYSLDMETQPQRVRDELREKVRRWRNEMLRTL
                     PQPVIARITGWCLGGALTVTAGCDIAIASEEALFGLPEVNFGHFPAGETTAVLTEHLQ
                     PKHGLYYALTGKMMTAHEAERIGLISKVVPRAILDNEVAEVAKCLAEKSPMALKAVKE
                     AWYYSWYSPPDVAFEISNLISQRTIREHGGRPGLEQFVQKKLRPVSGVMSLERK"
     gene            5372..>6358
                     /locus_tag="VFY96_10675"
     CDS             5372..>6358
                     /locus_tag="VFY96_10675"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5606970.1"
                     /translation="MKTSLLTALMFSLSIAVGWCQTAPSKSAVELAKYMAADRERLLY
                     DGAKKEGKLVWYTSLTIYKEMAKFFEAKYPGVAVELYRATAVNLVSRVLSEAQAKRYI
                     VDVVETTPGSLMLVRDNQLLLPYNSPHLSAYPEGSKDKAPGGLFYTAVDRESYAGIGY
                     NRNAIASADVPKSFDDLLRPSLKGKIGISNEEIATRVIGAMLKEKGDGFIKKLAGQEI
                     KQYSLPALGLNELIVSGEVPLSFTAVDSNVRLAASRGAPVAWLPGDLVPANAGSLGAF
                     LYTQHPHAALLFIDFMIGPEGQKLFAEKYGYGSPRKDYGFKRWYPEQGLSTYD"
BASE COUNT         1427 a         1805 c         1693 g         1433 t
ORIGIN      
        1 gatttcgcgc gccttaacct tgtcacccat ctggtcgatg actttgggcg atggaccgat
       61 gaagacgatt ccctcgtctt tgcagcgctg ggcaaatgcc ggattttccg ccaggaagcc
      121 atatcctggg tgtaccgcct cggcgccact tcttttgcaa acatcaataa tacggtcgat
      181 cgcgaggtaa ctttggatgg acggcgcagg tccgatcagg taggcgtaat ccgcatactt
      241 cacgtgcagc gactcgcggt cggcttcgga gtaaaccgcg acggactcaa tatctagctc
      301 gcggcaagca cgcgcgatgc gcacggcgat ctcgccccga ttggcgatca agatgcgctt
      361 aaacatcgaa tggggatgtc agggcgcagc atgctgcgcc ctgactaaag tggaatgttg
      421 ccatgctttt ttggcgggtg atggtcttgc ttgtttttca gcatatcgag agcgcgtatt
      481 aatgtcgggc gagtctcttc aggataaatg atcccgtcga tatatcccga ttccgccgct
      541 ttgaaagggt tggcaaacgt ctcgcggtat ttgccgatta gctgctcacg cgcatttgcc
      601 gggtcagcgg ccttttgcaa ttcgtcgcga aacacgatgt tgatcgcacc ttcggggccc
      661 atgacggcga tttccgcggt cggataagca aagttgagat cgccgcgcag atgtttgctc
      721 gccataacga tatagccacc gccgtaggcc ttgcgcgtga cgacggtgac cttcggaacg
      781 gtcgcttccg cataagcgta gagcagcttt gcgccatgcc gaatgatgcc gtgatattcc
      841 tgctccgttc cgggcaaaaa tcccggcacg tcgacaaacg tcaccagggg aatattgaag
      901 cagtcgcaga aacgaataaa ccgcgccgcc ttcaccgctg cattgatatc caggcaaccg
      961 gccaggttcg cgggctgatt ggcaacgata ccgaccgagc gaccaccgag atgagcgaac
     1021 ccaactaaaa tattccgggc aaattctgcc tgcacttcat agaaatagcc ttcatcgacg
     1081 accgcggtaa tcaactcttt catgtcatag ggccgattgg gattatcggg aatcagggtt
     1141 ctaagccgtt tgtcgcgccg cagcggatca tcggtgttcg gccggaatgg cggatcctct
     1201 tggttgtttc ccggcaaaaa tccaatcagg tcccggatca tcaacaggca atctttctcc
     1261 gaacctgcgg cgaaatgcgc gaccccgcta cggcccgcgt gcgtctcggc gccgcccaat
     1321 gtttcttttg aaacttcttc atgagtgacg gttttaatga cgtccggacc ggtgataaac
     1381 atgtagctgt tggttttcag catgaaaatg aaatcggtga tcgcgggtga ataaaccgcg
     1441 ccgccggcgc atggacccag aatcgctgtg atctgaggga ccacgcccga ggccatgaca
     1501 ttgcgttgaa agatttctcc gtatccggcc aggctatcga cgccttcctg aatgcgcgcg
     1561 ccgccggaat cgttaatgcc gataatcggc gcaccgttct tgagcgccat atccatcact
     1621 ttgcagatct tgtccgccac cacacggctc aaactcccgc caaaaaccgt gaaatcctgc
     1681 gaatacacgt acactaggcg actatcaatg gcgccataac cggtcaccac cgcatcgccg
     1741 aaaaatttct gttgctccat gccgaaatcg gtgcattcgt gggtgcgcaa acgatcgagc
     1801 tccacaaagc tgccgctatc gagcaaaata ttcaggcgct cacgcgcgag aagtttcccc
     1861 tgctcatgtt gccggcgcgc gcgctcagca ccgccgccag cttcggctct ttcgttcagc
     1921 tcccggagct tgtccagatt gttgatcgtc accatgaagg aagatcctta gaggggaaat
     1981 cagcgttatt ccgccaacga gtggcggcat cataacagac acctttgagc tgtcaacaga
     2041 atgttacggc gcgcgcgtcg aagttgcgct cgcaaagagg ccgcgttact atcgttgaac
     2101 acgaattcgc atgccccgtt cacgaccgga aaaattttaa tgttcgaaac tcaaactgcc
     2161 cggcaaaaga aatcgaaact cgctggcgac gagcgcgagg ccgtaaaact gcgcggcgat
     2221 caggccattt tgctcgtcgc agccagcgag ggcgatgcta acatgctcta cgcggttggc
     2281 tttttcgtcc ccgacccatt catttttttt cagcacaaag aaaaaaaatt cgcggtgctg
     2341 agcgatctcg agatcgatcg cgccaaaaag caggctcacg tgcatcgggt gctttcactt
     2401 tcgctttatc aacgcaagct gcgccagctc ggcaagcctg ccgcaatgat cgatgttctt
     2461 gatttgatct tccgcgaaag aggcattcgc tcactgatcg ttccggcaaa tttttccgcg
     2521 ctgctttccg atcagctccg cgccaagggt tatagcgtgc agatcaagcg cgatccattt
     2581 tggcccgagc gcgaaactaa aaatcgcgtc gaggtcgcgc agatcacgga gtccttgcgc
     2641 atcgcaagac tcggccttga agccggtatt cgaatgctca agagaacgcg gaataaacgc
     2701 gacggttatc tgtatttgaa cggcaggcga ctgacttctg agatgctcaa gaccgccgtg
     2761 aatacgacga ttatggcgca gggctggttg cccagtcaca cgatcatttc atccggcaac
     2821 cagtgtgtcg atccccatca tgaaggcagt ggtccgatca aagcggatac ctcgatcatc
     2881 tttgatatct ttccacgctc gcagcacaac ggctacttcg gcgatctcag ccggaccgtg
     2941 gtgcgcggcc gggcgtcgga taagctcaag gagatttacg ccaccgtgca agccggccag
     3001 cagatcggct accggcaaat tcgtgacggc gtcaatggta aagaggtgca ccagaatatt
     3061 ttggcgctgt tcgaagcgcg cggctttcat tccggacgga tcaacggccg tatgcaaggt
     3121 ttttttcacg gcacgggtca cggtctcgga ctcgatatcc acgaaccgcc acgcattgca
     3181 ccggtcgacg ccatcttgcg cgccgggcac gtggtcacgg tcgagcctgg cctctattat
     3241 ctcggcatcg gaggagtgcg tctagaagac gtcgtagtgg ttactgcgaa aggcaatcgc
     3301 aacctgaccg actgcccgca gttcctcgaa atttaaggtt ctctccaagc cattgcggct
     3361 taagcgaaag tcggacacgc aacgcatctc ttgcggccat gaacgacagt gagattgccg
     3421 aactcatcgc taagctcgcc catcacgaca aacccaccct gcgagcggcc gtcgacgcac
     3481 taatcctcct cgccactgaa ttttccaccg tccgcgagac tttgatccgc cgtttgcctg
     3541 aggcagagcc cagcaagaaa tggccgatcg cttacatcct cgcccatttg ccgcaacctg
     3601 gccagccggt catccgaagc ttgctcgatg cgctcgatca tcgcgatgcg gatattcgtt
     3661 gggcgatcgc cctacttctg gtgcgcgtcg caaaattgga acctgacagg cttaacgaac
     3721 tgatagatct ctgcgccacc gggaccgcca atcagaagcg tatggcgctt tactgtattc
     3781 gcgatctgac gctgtcggat ccgcagagct tggccgctct ccttcgagcg cttgtcgatg
     3841 tcgattcgac ggttagagtc gccgcggcaa ttgctttgca gtcccgttca gacctggacc
     3901 gggctggcag aaatttttta ttgcagactt atctgaacga tgcggagcta aaagttcgca
     3961 acaccgccgc cattgcactg gctaatctgg gagagacctc ctcggagttc ctatgtgctt
     4021 tgcaggaagc tgtgctgggt gcagatgcac aagtgagaaa aaccgccatg aaggctttgg
     4081 atctcataaa aaaaagatcc gcttcagcgg aaaattaatc ccgctaaagc ggatcctagg
     4141 tggactgagg gggattccca ggctccaggc ccgaatcccc gtcaaagatt ccgcctagag
     4201 cctaagtctc agccacctcg cagacagtac attgatcaat catgtctgga ccacctcctt
     4261 tccctgctga attttagttt acgaaagtgg ttttcaactg tcaatggaaa cccgcgtatt
     4321 ttgcgcgcga gaaaaaactt tttgctcgtc cgttcgaact aatttttact gcccttcggt
     4381 tgcatcgctg cgcagcaagc gctcgccatc gcaacacgac tgacgaagag aaccacctaa
     4441 gagctatttt ctctccaagc tcatcactcc ggaaacaggc cgaagctttt tttgcacgaa
     4501 ctgttcgaga cccgggcgcc cgccatgttc gcgaatggtc cgctgagaaa tcaaattgga
     4561 gatctcgaag gcgacatccg gcggcgagta ccagctgtag taccacgctt ctttcaccgc
     4621 tttcagagcc atgggacttt tttcggcaag gcacttcgcg acttcggcaa cttcgttgtc
     4681 caatatggcg cgcggcacta ctttggagat taaaccgata cgctccgcct cgtgcgccgt
     4741 catcatctta cccgtgagcg cgtaatacag tccgtgtttt ggctgaaggt gttcggtcaa
     4801 aactgcggtg gtttcgccgg caggaaaatg accgaaattg acttccggca agccgaagag
     4861 cgcttcctcc gaggcaatgg cgatgtcgca cccggccgtg accgtcaagg cgccaccgag
     4921 gcaccagccg gtgatgcgag cgatgaccgg ctgcggcaag gtcctgagca tctcgttgcg
     4981 ccagcgccgc accttttcgc gcagctcgtc acgcacccgt tgtggctgag tttccatgtc
     5041 caatgaatat tgtttcaaat cctgccccgc acaaaaattt tccccgacgc cggtcagcac
     5101 aatgacacgc gtctcggagt cgtagcgtaa atccgtgagc agttcgaaca tctcgcggtg
     5161 gagttgcgga ctcattgcgt tgcgcttttc cggacgattg aacgcgatgg tggtgatgcc
     5221 gttttccttc gtcaccagta atgtctcgta gctgatcgtc atgttttcat ctcctttata
     5281 gtttgtgatg cgttaatgtc gcgctggtta attgacatag cactccgtta ttgaatattg
     5341 ctaacaagat cgctgcttct ccggagacaa aatgaaaact tcgctgttaa ccgctctaat
     5401 gttttcgctc agcatcgccg tgggctggtg ccagacggca cccagcaaga gcgcggtcga
     5461 gttggcgaag tacatggccg cggatcgcga gcgcctgctt tatgatggcg caaaaaagga
     5521 aggcaaactc gtctggtaca cgtcgctcac gatctacaag gaaatggcta aattcttcga
     5581 agccaaatat cccggtgttg ccgtggagct atatcgggcg acggccgtca atctcgtcag
     5641 ccgagtattg agcgaagcgc aagcgaaacg ctacatcgtc gatgtcgtcg aaaccacgcc
     5701 gggctcgctc atgctggtgc gcgacaatca gcttctgttg ccctataact cgcctcacct
     5761 gagcgcgtat ccggaaggct ccaaagacaa ggcgcccggc ggtctgttct acaccgcggt
     5821 tgatcgcgaa tcctatgctg ggatcggcta caaccggaac gcaatcgcca gtgccgatgt
     5881 gccgaagagc ttcgatgacc tgctgagacc ctcattaaaa ggaaaaatcg gtatcagcaa
     5941 cgaagaaatc gcgacccgtg tgatcggcgc catgctgaaa gaaaaaggcg acgggttcat
     6001 caagaagtta gccgggcaag agatcaagca atatagcctg ccggcgcttg gcctgaatga
     6061 attgatcgtt tccggcgagg taccgctctc gtttactgcc gtcgatagca acgtgcgatt
     6121 ggcggcgtcc cgaggcgctc cggtcgcatg gcttcccggt gatctagttc ccgccaatgc
     6181 gggcagtttg ggtgcatttt tatacacaca gcatccgcac gctgcgctct tgttcatcga
     6241 cttcatgatc ggcccggaag gacaaaagtt gttcgcagaa aaatacggct acggcagccc
     6301 gcgaaaggat tacggcttta aacggtggta tcccgaacag ggattatcca cttatgat
//
LOCUS       DASULZ010000519         2129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_264760, whole genome shotgun sequence.
ACCESSION   DASULZ010000519 DASULZ010000000
VERSION     DASULZ010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264760"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..1267
                     /locus_tag="VFY96_10680"
     CDS             257..1267
                     /locus_tag="VFY96_10680"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5606971.1"
                     /translation="MGFRSLSVGNLIVASFLIGFSSAAAQQPLDAARKEGQVVFYASM
                     EAQSAQRLSAAFEKKYPPIKVDTVRIGSEKMATRLIAEAQARKMSVDVVHQSAFDFYG
                     VLQKGIFDSYLSPERSAFPADYRDDKGIWTLHSATLNVIAYNTRRFPGLPVPKGFWDL
                     TAPNWKSQLIMDENESKWMAGMMTYWGEAKTLEFLRKLATQDIQFRTGHSLIQTMVAA
                     GERPVAVVAFANGVDRLKKDGAPIDWIAPEPVIGLTFGLAVVKGAPHPSAARLFVDFL
                     LSREGQEILGAAGYFAPRSDVISPILSEAPPKTKIVPLPMTLAVRYNDYFQMYREIMG
                     LR"
     gene            1386..>2129
                     /locus_tag="VFY96_10685"
     CDS             1386..>2129
                     /locus_tag="VFY96_10685"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX5606972.1"
                     /translation="MTVYQAETGNFTIALAGDTMLTRKLVPFKEEGFLRLREIFRSAD
                     AAFANLEGTVHLWDEGTPGITQGTFMTTDPKLLEDLQWLGVNMISCANNHAFDYGEAG
                     VLANIRHLDHAGMMHAGTGKNLAEARAPGYLDTPNGRVALVSTTATFRPWNQAGEQRP
                     DLRGRPGINPLGFKTTYHVDAAALEQLQRISRELGFEMGKERARKHFYSDKEIADDKE
                     SEVDLLGHRFVQDQSFCIKTEANPRDVEDN"
BASE COUNT          519 a          570 c          595 g          445 t
ORIGIN      
        1 accagcgaaa aataagggtt gttcatttgc gattatttaa aagccaaccc tggaaaaggt
       61 tttggaacgg ggtctagcaa atcaccaaga ttgctaagtt tttctatgaa aaaaccgcag
      121 tgaagtcaag gtaacaggcc actcgccagc cctttccggt ctttcaaaga gaaagcttag
      181 attgacgatt aacgagcgtt ggtgctaatc ggatgagggt ctttcgaatc agccgcaacg
      241 gaccaaggag cgggcgatgg gattccgttc actttcggta ggcaatttaa tcgtcgcttc
      301 ttttctcatt ggtttcagct cggcggcggc acagcagccg ctcgatgcag cgaggaaaga
      361 aggacaggtc gttttttacg cctcgatgga agctcagtcg gcgcaacggc tcagcgcagc
      421 cttcgaaaaa aaatacccac ccattaaagt cgacaccgtg cgcattggct cggagaagat
      481 ggcgacccga ctgattgccg aagcgcaagc gcgcaagatg tcagtcgatg tcgtccacca
      541 atcggcattc gatttttatg gcgtgcttca gaaaggaatt ttcgacagct atctttctcc
      601 cgagcgcagc gcttttccgg ccgactacag agacgacaaa ggaatctgga cgctgcactc
      661 cgcgactctc aatgtgatcg cctacaatac gcgccgattt cccgggctgc cggtacctaa
      721 gggcttttgg gatttgacgg cgcccaattg gaaaagccaa ctcatcatgg acgaaaacga
      781 gagcaaatgg atggccggca tgatgacata ctggggcgag gcaaaaacgt tggaatttct
      841 acgcaagctc gccacccagg atattcagtt ccgcaccggt cactcgctaa tccagaccat
      901 ggtcgccgcc ggcgagcggc ccgtagccgt ggtggcgttc gccaacggcg tcgatcgcct
      961 aaaaaaagat ggggcgccta tcgactggat cgcccccgag ccggtcatcg gccttacgtt
     1021 tgggctcgca gtcgtcaagg gagcgcctca tccaagcgcg gcccgtctgt ttgtcgattt
     1081 tctgttatcg cgcgagggtc aggaaatact cggcgcggct ggctacttcg cgccgcgcag
     1141 cgatgtcatt tcgccgattc tcagcgaagc gccaccaaag acgaaaatcg tgccgctgcc
     1201 gatgacatta gccgtgcgtt acaacgacta ttttcaaatg taccgagaga tcatgggact
     1261 tcgatgattt cggaatctga gaatgtcgta ggagcgcatg gcatgcgcgc tctgtggatc
     1321 atccgagccg cacgaatcgc gaccaacaaa gaagccatta gtttaacgcc aacgggggct
     1381 aaaccgtgac ggtttaccaa gctgaaacgg gaaatttcac catcgcgctc gcgggcgaca
     1441 ctatgctcac gcgtaagctc gtccccttca aggaagaagg ttttttgcgc ttacgcgaga
     1501 tctttcggag cgccgatgcc gcctttgcca accttgaggg gaccgtccat ctctgggacg
     1561 aaggcacgcc tgggattacc cagggtactt tcatgacgac cgacccgaag ttactcgaag
     1621 atttgcaatg gctcggggtc aacatgattt cctgcgccaa caaccacgcc ttcgattacg
     1681 gcgaggctgg cgtgttggcg aatatccggc atctcgacca tgccggcatg atgcatgcgg
     1741 gaacgggcaa aaatctcgct gaggcaagag cgccaggata tctcgacaca ccaaacggaa
     1801 gggtggcgct agtctcgacc actgcgacgt tcaggccgtg gaaccaagcg ggtgaacaac
     1861 gacccgactt acgcggccgg cctggcataa atccgctcgg gttcaaaacc acttatcacg
     1921 tcgatgcggc ggcattggag caactgcagc ggatcagtcg cgagctcggc tttgaaatgg
     1981 ggaaggagcg cgcacgcaag catttttata gtgataagga gattgcggac gataaggaat
     2041 ccgaggtcga tttgctgggc catcgctttg tccaagatca gagtttttgc ataaagaccg
     2101 aagcaaaccc ccgagatgtc gaggacaat
//
LOCUS       DASULZ010000520         4270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_264772, whole genome shotgun sequence.
ACCESSION   DASULZ010000520 DASULZ010000000
VERSION     DASULZ010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264772"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..635
                     /locus_tag="VFY96_10690"
     CDS             171..635
                     /locus_tag="VFY96_10690"
                     /inference="COORDINATES: protein motif:HMM:NF023036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HEX5606973.1"
                     /translation="MQAKKTFLKLLITLILPGCASYQAQPLTVNHPAHPDAQAGVIAS
                     RSKTLAYTAADLASGAITRTAADSEGYDAHHARSASAPKTATGEGSVVAVVPATGQLV
                     IQHGDIKDFMGPMTMGYPTEPASLLEGLKPGDKVRFSIDTEKKVIVKIEKLS"
     gene            660..2081
                     /locus_tag="VFY96_10695"
     CDS             660..2081
                     /locus_tag="VFY96_10695"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX5606974.1"
                     /translation="MSCCRRSAVFVCWALFVSGCAGLDLQSGFSDVSSNVQERLAVPI
                     FWNNGTDLDCEASDRRRKLLSEKITADEAVQIALLNNRELQAVYSELGVAQADLVQAG
                     LLSNPIFDAAIKFPTSGRNSPDLELGAAMNFLNIFYIPLRKRVAAARFEEAKTRVTGA
                     VLDFAGRVRRAFYAYEADEQMLDLRHTVVQALSASFAIAGRLHQAGNISDLDFFSQRA
                     QLEAGRLALRAAEISVRQSREELNSLMGLWGSDLEWQSTERLPDIPNAPLETKDIERV
                     AIERSLDLAQARQRIIGTGEELGLTRWTTLLPDFSAGPHSERNDGTWEVGPKLDFLIP
                     LFDQGQARLGRTAAELRRLQQEYYDLAVRIRSRARQVRDRVEGATDRAMYYRDILLPL
                     HERIVNEAQLQYNAMQLGPLQLIRAREQQIETAVSYIAALRDYWLARAELEQMLTGSL
                     PSSTMLPSRAATSPSGSSVSEGH"
     gene            2103..3572
                     /locus_tag="VFY96_10700"
     CDS             2103..3572
                     /locus_tag="VFY96_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HEX5606975.1"
                     /translation="MKTKVSRRQVLGMGAATIAGVALSKATAHAINHDASQADFRPFR
                     GYSPQRGGEGPYWEKSYSGGPVNVKPLAAVLPGKGYTPVVVPNGAALSFRIIDGVKVF
                     HLIAEEVDHEFDAGLKAKCWGYNGRVNSTVIEAVEGERVRIYVTNRLSVATSVHWHGV
                     YLPNGMDGVGGLTQPYIKAGETVKYEFTLRQYGTQMFHSHHDEMTQMGMGLIGMFVIH
                     PRNPRPEHQVNRDFSLMISEWSIPAGTFRPNTLEMTDFNVLTINGKVFPSTAPLICKT
                     GEKVRLRLGNLGATDHHPMHIHGHHFRVTATDGEEIPLSAQWPESTVLVGVGQTRNIE
                     FISDAPGDWAFHCHMTHHVMNQMGHQFPNMVGIEPEGLEEKVRALLPAYMTMGSTGMD
                     MGKMAEGMPYPRNTIAMKGASGPFGDYISMGGLFTIIKVRDQLRSYDEDPGWYQHPPG
                     TVALQVNDEELRRDGIDVNKVNNSDSEAAEKKPNEHIGH"
     gene            3553..>4270
                     /locus_tag="VFY96_10705"
     CDS             3553..>4270
                     /locus_tag="VFY96_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606976.1"
                     /translation="MNISATNRLPNDVGTGERYRAWVMALLLGLLIGANNLIKILRDS
                     VFLGHHSVSELPYLYIMVAVLASVIIGTYTRYTAKVSITRLILSTNAIILSNLVFFWI
                     VLTYFNADWSHYAFYIWSAMATVIAVAQVWTLANSIFTPEEGKRSFGFIAAGGTVGGV
                     AASFGANWSIHLSIESNHLLWIVAGIYMAASVLVIWAEGRLIGTVHEAEIHSPNTAEN
                     NYRSGLGELLSGSSYLKAIAV"
BASE COUNT         1021 a         1088 c         1223 g          938 t
ORIGIN      
        1 tcgagcacgg cccgatcaaa gattacatgg acgcaatgac catggggtac aaagccagct
       61 ccacttcttt gctcaagggg ttgagcgtcg gcgatcaggt ggagttcacg ctcgatacta
      121 ccaagcgggt gattacaaaa attcaaaagc gccatgagta atgagggttt atgcaagcta
      181 agaaaacatt tttaaaactc ttaattacgc taatactgcc cggctgtgca tcgtatcaag
      241 cgcaaccgtt gacggtaaat catccagcgc acccggatgc acaggcaggt gtcatcgctt
      301 ctcgatccaa aaccctggcc tacacagccg cggatttggc ctcgggtgcg atcacccgga
      361 cggctgccga ttcagagggt tacgacgcgc atcacgcacg gagcgcaagc gctccgaaaa
      421 ccgcaactgg agagggaagc gtcgtcgcag tagttccggc gaccggacag cttgtcattc
      481 agcacggaga cataaaggat ttcatgggtc caatgaccat gggttatcca acagaaccgg
      541 caagcttgct cgaagggctc aagcccggag acaaggtgcg attttcgata gataccgaaa
      601 agaaagtaat cgtgaaaatt gaaaagctca gttgaggttt tgttcatcgg agaaacacaa
      661 tgagctgctg tcgaaggagt gcggtgtttg tgtgttgggc gctattcgtg agcgggtgtg
      721 caggcctcga tttgcaatcg ggtttttccg acgtcagctc caacgttcaa gaacgattgg
      781 ctgtgccgat cttctggaac aacggcacag atctcgattg tgaggctagc gatcgccgta
      841 ggaaattgtt gagcgagaaa attaccgccg atgaggccgt gcagatcgcg cttttgaaca
      901 accgggaact gcaggccgtg tattccgaac ttggcgtcgc gcaagcggac ttggttcaag
      961 ccggtctcct cagcaatccg atcttcgatg ccgcgataaa atttccgact tccggaagaa
     1021 acagtccgga ccttgaattg ggcgcagcta tgaacttcct caatatcttc tacataccgt
     1081 tgcgaaagcg cgttgccgca gcgcgattcg aggaagctaa aactcgggtt accggcgctg
     1141 tgctcgactt cgcgggtcga gtgcgccgcg ctttctatgc ttatgaagcc gacgagcaga
     1201 tgctcgattt gcgtcatacc gttgtgcaag ccctctctgc ttccttcgcc attgctggcc
     1261 gtcttcacca ggcgggcaat atctcggacc tcgatttttt cagccaacgg gctcagttgg
     1321 aagctggaag actagccctc agggcggcgg aaatctcagt tcgtcagagc cgagaagagc
     1381 tgaactcatt gatgggacta tgggggtcgg acctcgaatg gcaaagcacc gaacgattgc
     1441 cagacattcc gaacgcgccg ctagagacca aagacatcga acgtgttgcg atcgagcgca
     1501 gtctcgatct cgcgcaggca cgccaacgga ttatcggcac cggcgaggag ttaggattga
     1561 ctcgatggac gacgttgctg ccagacttta gcgccgggcc gcacagtgaa cgaaatgatg
     1621 gaacttggga agtgggaccc aaactcgatt ttctgattcc gttgttcgat caaggccagg
     1681 cgcgcttggg gcgaacggct gcggagctga ggcgtcttca gcaggagtat tatgatctgg
     1741 cggtgcgaat ccgttcccgg gcgcggcaag tgcgcgacag agtggaggga gcgactgatc
     1801 gcgccatgta ttaccgcgac attcttttgc cgctgcacga gcgcatcgtc aatgaggcgc
     1861 aacttcaata caacgccatg cagcttgggc cattgcagct catacgtgcg cgcgagcagc
     1921 agatcgagac cgcggtctcg tatatcgccg cgctgcgcga ttactggctt gcccgagctg
     1981 agttggaaca gatgctgacc ggctcactgc cgagctcgac gatgctcccg tccagagcgg
     2041 ccacgagtcc atctggcagc agtgtaagcg aagggcactg acgagattcg aaggagttaa
     2101 caatgaagac gaaagtgtct cggcgccaag tgctaggtat gggcgccgca actattgccg
     2161 gcgtagcttt atccaaagcg acggcgcacg cgataaatca cgacgcttcg caggcggatt
     2221 tccggccatt cagaggctat agcccgcaac gcggaggcga agggccctac tgggaaaaaa
     2281 gctacagcgg cggaccggtg aatgttaaac cactcgcggc agtgctacca ggaaaaggtt
     2341 acactccggt cgtcgttcca aatggtgcag ctttgtcttt tagaatcatt gacggcgtca
     2401 aagtctttca tttgatcgca gaggaagtcg accacgaatt cgacgcggga ttaaaggcca
     2461 aatgctgggg atacaatggc cgggtgaaca gtactgtgat cgaagcggtc gagggcgagc
     2521 gcgtgcggat ttatgtaacg aaccgcttat ctgttgcaac atcagtccat tggcatggcg
     2581 tttatctgcc caacggcatg gacggagttg gcggcctcac ccagccatac atcaaagccg
     2641 gtgaaacggt gaaatatgaa tttacgctgc ggcagtacgg aacgcagatg ttccactcgc
     2701 atcatgacga gatgacgcag atgggcatgg gtctcatcgg tatgtttgtg atccatccga
     2761 gaaatccgcg gcctgagcat caggtcaacc gcgatttttc gctgatgatc agcgaatggt
     2821 ccattccggc cgggacattc cgtcccaata cgctcgagat gaccgatttc aatgttctca
     2881 cgatcaacgg caaagtcttc ccatcgactg cgccgctgat ttgcaagacc ggcgaaaagg
     2941 tccggcttcg tttgggtaat ctgggcgcaa ccgatcatca cccgatgcac attcatgggc
     3001 atcatttccg cgtcaccgcg acggacggag aagaaatacc attgtctgcg cagtggcccg
     3061 aatcgaccgt tctcgtaggc gttggacaga ccaggaacat agaattcatc tccgacgctc
     3121 ccggtgactg ggcattccac tgccacatga cgcatcacgt catgaaccag atggggcacc
     3181 agtttccaaa catggttgga atcgaaccgg agggcttgga agaaaaggtt cgggctttgc
     3241 ttccggcgta tatgaccatg ggaagtacgg gtatggacat ggggaaaatg gccgagggca
     3301 tgccgtatcc gcggaatacg atcgcgatga agggggcatc gggtccgttc ggcgactata
     3361 tctcaatggg cggacttttc acgatcatca aagtccgcga tcaattgcgg agctatgacg
     3421 aggatccagg atggtaccaa catcccccag gaaccgtagc gctccaggtg aacgatgagg
     3481 agcttcgtcg cgatgggatc gatgtaaaca aagtcaacaa tagtgacagc gaagctgccg
     3541 agaagaaacc caatgaacat atcggccact aatcggctgc caaacgacgt tggtactggt
     3601 gaaaggtatc gcgcatgggt aatggctttg cttcttggcc tgctgattgg cgcaaacaac
     3661 ctcatcaaaa tcctacgtga cagcgtattt ttggggcacc attcagtgtc cgagttgccg
     3721 tatctttaca taatggttgc tgtgctagcg agtgtgatca tcggaacgta tacgagatac
     3781 accgcgaaag tctccatcac gcggcttatt ctatcgacaa atgcgatcat tctctcgaat
     3841 ctagtctttt tctggattgt attaacctat ttcaacgccg actggagcca ctacgccttc
     3901 tacatttggt ctgcaatggc gaccgttatc gccgtggcgc aggtttggac gcttgctaat
     3961 agcattttta ctcccgagga gggtaaacga tcttttggtt ttatcgcggc cggagggacc
     4021 gtgggtggag tggccgctag tttcggagct aattggtcaa ttcatctctc catcgagagc
     4081 aatcatctgc tctggattgt tgcggggatc tacatggccg cgtccgtgct agtgatttgg
     4141 gcggaagggc ggcttattgg gaccgtccac gaagcagaaa tccacagtcc gaatacagca
     4201 gaaaacaatt accgaagcgg gttgggtgag ttgctctctg gttccagtta tctgaaggcg
     4261 atcgctgtta
//
LOCUS       DASULZ010000521         2551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_163681, whole genome shotgun sequence.
ACCESSION   DASULZ010000521 DASULZ010000000
VERSION     DASULZ010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163681"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1671
                     /locus_tag="VFY96_10710"
     CDS             <1..1671
                     /locus_tag="VFY96_10710"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX5606977.1"
                     /translation="SHKLLVRGGFIRQVSRGIYDYFPLALKVIRKIENIVREEMDRAG
                     AQELLMPIASPAELWQESGRWDVYGKELIRFKDRNDRDFCLGPTHEEIVTDLVRRAVR
                     SYRELPFNLYQIQTKFRDEVRPRFGLMRGREFIMKDAYSFHTDIDDCKREYDNMFVAY
                     RRIFDRCGLKYRPVEADTGAIGGSLSHEFQVLAESGEDAIVSCNNCDYAANLEKAAVR
                     PQRAAARDLNIEAPGLQKVSTPGKRSVAEVAEFLKLAPEKFIKTMIYQVNGDELVAAL
                     VRGDHEINQVKLQAALGCREIALASETAVVAATGVTPGYLGPIGLKLRTIADLSVQSM
                     RGAVTGANEPDAHYIEVDQERDFTPTTFADIRLALAGDHCPRCDQGRLEAHRGIEVGQ
                     VFYLGKKYSELMGATYLDAEGRERPIEMGCYGIGISRLVAAAVEQNHDDNGIIWPLSI
                     APFQVLLLPINYTDNATREAADVLYGELRQRGLEVLLDDRDERPGVKFKDGDLIGIPL
                     RITIGAKGLQKGSLELRWRREGKSIDIPVTGAAQTIHSHLSKSFQQTT"
     gene            1668..2441
                     /gene="pyrF"
                     /locus_tag="VFY96_10715"
     CDS             1668..2441
                     /gene="pyrF"
                     /locus_tag="VFY96_10715"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX5606978.1"
                     /translation="MTSKLELLRARRPTRERLIVALDVDDLNQAKDLVQALASEVGMF
                     KVGKQLFTHAGPQAVRLIQELGGEVFLDLKFHDIPNTVAKAAVEATRMGVKMFNVHAS
                     GSLEMMRMTVKEVRRVCRQEKRRRPIMLAVTVLTSLNQDDLKRVGIDGRVAAQVVRLA
                     LLTNEAGMDGVVASPHEVRAIRDCCGRRFIIVTPGIRPADSSRNDQQRIMTPAAAVKA
                     SVDYIVVGRPILEAKDPVAAARSIVTEMKSSSKEPVGVS"
BASE COUNT          589 a          701 c          732 g          529 t
ORIGIN      
        1 agccacaagc tgctcgtgcg cggcggcttt atccgccaag tcagccgcgg catctacgac
       61 tatttcccgc tcgctttaaa agtcattcgc aagatagaaa acatcgtcag agaagaaatg
      121 gatcgcgccg gcgcgcagga gctattgatg ccgatcgctt cgcccgccga actctggcaa
      181 gaaagcggcc gctgggacgt ttacggcaag gaactgattc gcttcaaaga tcgcaacgac
      241 cgagactttt gcttgggccc gacccacgag gaaattgtta ccgacctggt gcgccgcgcc
      301 gtgcgatcgt atcgggagtt gccgtttaat ctttatcaaa tccaaaccaa atttcgcgat
      361 gaagtgcggc cgcgcttcgg ccttatgcgc ggacgagagt tcatcatgaa ggacgcctat
      421 agctttcaca ccgacattga cgactgcaag cgcgaatacg ataacatgtt tgtggcctac
      481 cggcgcattt tcgatcgctg cgggctaaag taccggcctg tggaagccga caccggcgct
      541 atcggcggca gcctgtccca tgaattccaa gtcctcgcag aatcagggga agacgccatt
      601 gtgagctgta ataattgcga ctacgcagcg aaccttgaaa aagccgcagt caggccccag
      661 cgagcggcag cgcgcgatct taacatagag gcgcctggtc ttcagaaggt ctcgactccg
      721 ggaaaaagga gcgttgccga ggtcgctgaa ttcctcaagc ttgcgcccga gaagtttatc
      781 aagacaatga tctatcaagt caacggtgat gaactggtag cggctctggt gcgcggcgac
      841 catgagatca accaggtcaa actacaagcg gcgctgggct gccgtgaaat cgcgcttgcc
      901 agtgaaacag ccgttgtcgc ggcaaccggt gtcaccccgg gctatctagg acccatcggc
      961 ttgaaactgc gaaccattgc cgatctttcc gttcagtcga tgcgcggcgc ggtgacggga
     1021 gccaacgaac ccgacgctca ctatatcgag gtcgatcagg agcgtgattt taccccgacg
     1081 acttttgccg atatcagact ggcgctagcc ggagaccatt gcccccgctg cgatcaaggc
     1141 cggctcgaag cccaccgcgg catcgaagtg ggacaggtgt tttatcttgg aaaaaagtat
     1201 agcgaattga tgggcgctac ttatttagat gccgaaggcc gcgagcgtcc gatcgaaatg
     1261 ggttgctatg gtatcggcat cagccgcttg gtcgccgccg ctgtcgagca gaatcatgat
     1321 gacaatggca ttatctggcc gttatcgatt gcaccgttcc aagttttact actgccgatc
     1381 aactatacgg acaatgcgac gagagaagcg gccgacgtgt tgtatgggga attgcgccag
     1441 cgcgggctcg aagtgcttct cgacgaccgg gatgaacggc ccggagtcaa attcaaagac
     1501 ggtgatctaa tcggaatccc tttgaggatt accatcggcg ccaaaggatt gcagaaaggc
     1561 agtctcgaat tgcgctggcg tcgtgaaggc aaatcgatag acataccggt tactggagcc
     1621 gcacaaacga ttcacagcca tctcagcaaa tcgttccagc aaacgacatg acctccaaac
     1681 tggaactgct ccgcgcacgg cggccgacgc gcgagcgtct gattgtcgct ctcgatgtcg
     1741 acgatctcaa tcaggccaag gacttggtgc aagcgcttgc cagtgaagtg ggcatgttca
     1801 aagtcggcaa gcagctgttc actcatgctg ggccgcaagc ggtgcgcctg attcaagagc
     1861 ttggcggcga ggtttttctc gatcttaaat ttcacgatat tcccaatacg gtagccaaag
     1921 cggccgtgga ggctactcgc atgggcgtca aaatgttcaa cgttcatgct tccggcagtc
     1981 tcgaaatgat gcgcatgacc gtcaaagaag tacggcgcgt atgccggcag gaaaagcgac
     2041 gcaggccgat catgctggcc gtgacggtgt tgaccagcct caatcaagac gatctcaaac
     2101 gggtaggcat cgacgggcgt gtggctgctc aggtggtgcg cttggcgttg ttaaccaacg
     2161 aagcggggat ggacggcgtg gttgcgtctc cgcacgaggt ccgagctatt cgggattgct
     2221 gcggccggcg ttttatcatc gttactcccg gcatccgtcc ggcggacagt agccgaaacg
     2281 atcagcagcg catcatgacg cctgcggctg ccgtcaaagc tagcgtcgac tatattgtcg
     2341 tcggacgtcc tattctcgag gccaaagatc cagttgccgc cgctcgctcg atcgtcaccg
     2401 agatgaaatc ctccagtaag gaacctgtcg gcgtcagctg attggaatct cgtcagcgat
     2461 tcacgtcgct tgctggattt gcgcccgaca ttattttccc caataacgaa agaagtgatg
     2521 cactggacca tggccgcttc cgaccggaaa g
//
LOCUS       DASULZ010000522         2424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_570181, whole genome shotgun sequence.
ACCESSION   DASULZ010000522 DASULZ010000000
VERSION     DASULZ010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570181"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1319
                     /locus_tag="VFY96_10720"
     CDS             <1..1319
                     /locus_tag="VFY96_10720"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5606979.1"
                     /translation="PYKIDHTYMEALQVYTNTVPCGFWRAPGAIQAVFASESHIDLIA
                     RALNMDPAKFRLINLVGEGEENALGKSWKGVKAKETLEAALSAGGWNNPKRANVGRGV
                     AMYERGTGAGKVWVNLTAELDGSLTVFTVAGDQGTGLQTILCQTVAAEMGVPYEEVRC
                     KIGNTSDVSYNVDVGFGGSRSTNINSAAAYQASGELRRKLTEQAAKMFGCDADQITYK
                     NGKFSIATGKSKPLSLSDVVKAHGAPVTVSVETEVPRKGSSTSFVAQVAEVEVDSETG
                     RVKLNKFVTAHDVGTIINPLGHQGQIDGEAVMAIGSSLMEELIHDQGKVTTTNLGEYR
                     IPNIADIPQFKTVLVKGNNGPGPYEAKGIGEHANVAPPAAIANAVQDACGVRLFEIPV
                     SAERLYQQLKEGNGRRQPLDTAARPSSQDGRKTVRAQHAAPFVRKP"
     gene            1316..2317
                     /locus_tag="VFY96_10725"
     CDS             1316..2317
                     /locus_tag="VFY96_10725"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606980.1"
                     /translation="MNYKYGSLIWSVSFLLLVWPIHVQAQDKIKVAYSSTDTLNQIWT
                     IAQDAGFYKKNGLDVELVYIGSTTVGIAAIVSQDVQVGNAAGSGVANAAVRGADAVSV
                     ACTINVLAYELVVLDSIKSAEDLKGKAIGISRFGSVSDVAARELLKGLGLRPMEDVKI
                     LQVGGAAERAAGFSRGVIAGFPSPPGNVSLIPGGLPHRILADMGDLKKPYPLPFICAV
                     TTKSYLAKNRSIVKRVVMSLIEASHYFKTNREGSQKIIAKYLRSANKAYLDSSYDSTV
                     KILERVPYTSREGMKIQLDEALKQAPNSKVTVDSLIDDSVVRELEKEGFIDKVYGKQ"
BASE COUNT          604 a          611 c          747 g          462 t
ORIGIN      
        1 gaccctataa gatcgaccat acctatatgg aagcgttgca agtctacacg aatacggtgc
       61 cgtgcggctt ctggcgggcg ccgggagcga ttcaggccgt gttcgcgtcg gaatcgcaca
      121 tcgatctgat cgcgcgcgct ctcaatatgg atccggcgaa atttcggctg atcaatctgg
      181 tcggcgaagg cgaagagaac gctttgggca aatcatggaa aggggtcaaa gcgaaggaga
      241 cgttggaggc cgcactgagc gctggcggtt ggaacaatcc gaaacgggcc aatgtcgggc
      301 gcggcgtggc gatgtatgaa cgcggcacgg gagcgggaaa agtctgggtg aatctcacgg
      361 ccgagctcga cggaagcttg accgtgttca ccgtggcggg cgatcagggt acggggttac
      421 aaacgatatt gtgccaaacg gttgccgcgg aaatgggcgt gccttacgag gaggtgcgtt
      481 gcaagatcgg caacacctcg gatgtctcgt acaacgtcga cgtcggtttc ggcggcagtc
      541 gctcgaccaa catcaatagc gcggcggcgt accaagcgag cggggagctc cgcagaaagc
      601 tgacggagca agcggccaag atgttcggct gtgacgcgga tcaaatcact tacaagaacg
      661 gcaagttttc aatcgcgacc ggaaaaagca aaccactgag cttaagcgat gtcgtgaaag
      721 cccacggcgc tccggtgacc gtgagcgtcg agaccgaagt gccgcgcaaa ggatcgtcga
      781 catctttcgt cgcacaggtc gccgaggtcg aagtggattc ggagaccggc agagtcaaat
      841 tgaataaatt cgtcaccgct cacgatgtcg gtacgatcat caatccgctc ggccatcagg
      901 ggcaaatcga tggggaggcc gtaatggcga tcggctcgag cctaatggag gagttgatcc
      961 atgatcaggg gaaagtcacg acgaccaatt taggcgagta tagaattccc aatattgcgg
     1021 atattccgca gttcaagact gtgctggtaa aaggaaacaa cggtcccgga ccgtacgaag
     1081 cgaaaggcat cggcgaacac gccaatgtcg caccgccagc ggcaatagcc aacgccgtgc
     1141 aggacgcgtg cggcgtgcgc ttattcgaga ttccagtaag tgcggagcgg ctgtaccagc
     1201 agttgaaaga aggcaacggg cgtaggcaac ccttggatac ggcggctagg ccgtctagtc
     1261 aggatggacg gaaaactgta cgggcgcagc atgctgcgcc cttcgtgagg aagccatgaa
     1321 ctataaatat ggaagtttga tttggtcggt ttcatttctg cttctcgttt ggccgattca
     1381 cgtccaagcg caggacaaga tcaaggtcgc ttacagctcc acggacacct tgaaccagat
     1441 ctggacgatc gcgcaggatg ccgggtttta caagaagaac ggcttggacg tcgagttggt
     1501 ttacatcggc agcaccacgg tcgggattgc tgcgattgtc tcgcaggatg ttcaggtcgg
     1561 gaatgctgcg gggagcgggg tcgccaatgc agcagtgcgc ggagcggacg cggtctccgt
     1621 cgcctgcacc atcaatgtgc tcgcatatga gcttgtggtg ctcgacagta taaaatcggc
     1681 cgaagatctc aaaggaaagg cgatcggcat cagccggttc ggaagtgtct ccgatgttgc
     1741 cgcgcgggaa ttgcttaaag gattgggatt gcggccgatg gaagacgtca aaattctcca
     1801 agtcggcggc gctgccgagc gtgccgctgg gttcagccgc ggtgtgatcg ccgggtttcc
     1861 ttcgccgcca gggaacgtca gcctcatccc gggcggactg ccgcaccgca tcctcgccga
     1921 tatgggagat ctgaaaaagc cgtatccgct gccgttcatc tgcgccgtga cgaccaagag
     1981 ttatcttgcc aaaaatcgtt cgatcgtgaa gcgagtggtc atgtcgttga tcgaagcctc
     2041 gcactacttc aaaaccaaca gagagggaag tcagaagatc atcgcgaagt acctacgcag
     2101 cgccaacaag gcttatctgg acagttccta tgattcgacg gtgaaaattc tcgagcgcgt
     2161 tccctatacc agccgcgaag gaatgaaaat tcaactcgac gaagcgctga aacaagcgcc
     2221 caacagcaaa gtcaccgtag atagcctgat cgacgatagc gttgtccgcg aactggaaaa
     2281 ggaaggcttt attgacaaag tctatggcaa acaataggca ctgccagggg cagagagatc
     2341 actgataaag ccggaaagtg tgctagcacg agcctgaatt cggaagaaat tgaatgacgt
     2401 gtataagtca acacatcatc tgac
//
LOCUS       DASULZ010000523         2118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_874527, whole genome shotgun sequence.
ACCESSION   DASULZ010000523 DASULZ010000000
VERSION     DASULZ010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874527"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(289..495)
                     /gene="rpoZ"
                     /locus_tag="VFY96_10730"
     CDS             complement(289..495)
                     /gene="rpoZ"
                     /locus_tag="VFY96_10730"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX5606981.1"
                     /translation="MARITVEDCLDKVSSRFDLVLLASKRARQLRMGAKPCIESDNRD
                     IVVALREIAAGKVRWIPPTPEPAK"
     gene            complement(1155..1637)
                     /gene="trxC"
                     /locus_tag="VFY96_10735"
     CDS             complement(1155..1637)
                     /gene="trxC"
                     /locus_tag="VFY96_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HEX5606982.1"
                     /translation="MADTKLIDCPSCGAINRVPLSKIAQGLQPNCGRCKKPLLMDSKP
                     VTVTDGTFASAVERSPLPVLLDMWAPWCGPCRIVAPVIEELAQKMAGRVRVGKLNVDE
                     NPATAARFGVQSIPTLLVLNEGREVDRIVGAQPKAVIVQRLEQAIRTRNQRRYQAGER
                     "
BASE COUNT          512 a          552 c          550 g          504 t
ORIGIN      
        1 atagttttgt acccatgttc tcctcctatg agagaatgtt ttgtctgaaa ccctgggaat
       61 tagactgcca ctcggcagaa catgggttaa ccaatactga aaccacgctc tggtcggttt
      121 gtccgttagg aatcgttcgc aaggagagag acggattaga ggccttcgag gtatttcttc
      181 ccatttcaga aggccattta ctgagatgga tagacccagc ctacgggtta aaaccggcaa
      241 tgtcaatcag ctggatgttg ccagagtgct cactgcggcg gggggtctct attttgccgg
      301 ttcgggcgta ggaggtatcc atcgcacctt gccggcggcg atctcgcgca gcgcgacaac
      361 aatgtcacgg ttatctgatt cgatgcaagg cttcgctccc attcggagct gtcgggctcg
      421 tttcgaggcg agcaagacga gatcaaaacg gctggaaacc ttatcgagac aatcttcaac
      481 ggttattctt gccataagaa acccactcta ggcttagccg tcgtggaaca caaggaaaat
      541 ctttaagctc gcgcatcagc accgagttcg cctaccaata gaaacgattc cagcgactta
      601 ctcgaagaaa gccgtctaat ttaattgttt tgccgcttct tgcggagcgc atccccgcgg
      661 tggcaaccgc atactggcga tatcgcttca ttgcgttcgg atgacgaaaa gcttagtgtg
      721 ctgatcgtga gcagccgaaa acaacaaaag attttcaaaa taaaggagtc ttaccgtcag
      781 cagttttacg tcacagtgat cacgttagac ccgtggagaa actcctggac atggaaaggt
      841 cacattgatt ttgatgcagg cattcatttg ctatccacca atcgcagctt cgccagcgcc
      901 gaagatgcga aggatcacat gcgccaaagc gcgcatcaat gcattgacaa ccggctcgga
      961 tagcgaactc gacgtcaggg gatcaaatgg cctttctgag cagcagcgcc gaaaacgagc
     1021 tcttgaacga gctgatctca aaccaaccct gaaattggat acgggaatgg gtgttaacgc
     1081 caatgacttg cacgccgatt ttgatttcgt cagtccgacg gagcgcgacg atggactggc
     1141 ttcatcggtg aacgtcacct ctcgccggct tgatatcgtc tctggtttcg ggtgcgtata
     1201 gcctgttcta gccgctgcac gatcaccgct ttcggctgcg cgccgacaat acgatcgacc
     1261 tctcgtcctt cgttgagtac gagcagagtc gggatactct gaacgccgaa ccgtgcggcc
     1321 gtggccggat tctcatcgac attgagcttg ccgacgcgca ctcgccctgc catcttttgc
     1381 gctagttctt cgatcaccgg cgcgacgatc cggcaagggc cgcaccatgg cgcccacata
     1441 tcgagcagca ccggcaacgg cgagcgctcg actgctgacg cgaaggtccc atctgttacc
     1501 gtcactggct tgctatccat aagcaaaggt tttttgcacc ggccgcaatt gggttgcagc
     1561 ccctgagcaa tctttgacaa aggcacccga ttgatcgcgc cgcatgacgg acagtcgatt
     1621 aattttgtat cggccatttc ttccaaatcc tctctttaat tgaagcttag cttgctcctg
     1681 cttcgatttc accatcctcc gtccgagaag tgccgctcag ccgagtatga gaccgtcatc
     1741 gtgttatcga cctgtcttta tagtggaagc gttttctact cgggtccgga ggaatctgac
     1801 aatcacgccg tttttctaag tggctgatgt caagcagtgg catgttcgtt gcggcatatt
     1861 tccttaggaa agagggcgaa aaaaatataa ttaggaattc attcgacaag acgctaatga
     1921 aaagctttgc ggcggtagga ctcgcagcgg cgttatccct cggcgcggga agcgcgtacg
     1981 ctcagtcgca caagttaaca tcggccaaaa acaaatgcgg cccgccgaga ggggctagag
     2041 cgtcgagaaa aatattatgg aaatgaggcc tacaatgaca acaatgacaa aattggcgag
     2101 gtggatgatt tgatcttt
//
LOCUS       DASULZ010000524         2260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1482934, whole genome shotgun sequence.
ACCESSION   DASULZ010000524 DASULZ010000000
VERSION     DASULZ010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482934"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VFY96_10740"
     CDS             complement(<1..340)
                     /locus_tag="VFY96_10740"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5606983.1"
                     /translation="MKFGIFVFGDNHPGLGRSNQRYYEEVMTMAEWAEELDFDSFWLG
                     EHHFYWYGTCVSPPMLIAALGQRTKKIRLGPAVSVLPFRHPLIVAEEYGLADNLCGGR
                     LDFAIGSGFSP"
     gene            complement(337..471)
                     /locus_tag="VFY96_10745"
     CDS             complement(337..471)
                     /locus_tag="VFY96_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5606984.1"
                     /translation="MIEPAICIPNNAGIAAHYDDLDNLYRETRGTDVHYGYFTVQDYQ
                     "
     gene            complement(542..1534)
                     /locus_tag="VFY96_10750"
     CDS             complement(542..1534)
                     /locus_tag="VFY96_10750"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5606985.1"
                     /translation="MKRRIFSNIALALSMTLSLSTYSAHAKSYIAYISDSSNSSVVYW
                     IAKEAGIFKKHGLDFETIFIDGSVRGIQSLLAGDLGYTGAVGPAVINAKLAGGDIAIV
                     QSQMNTLTYFVIGNSSIKSPEELRGKTAAVHIPGTSADFAMRLALNKVGIAYKDIKAV
                     TLGGAPARLGATINGQADFTIVSEGEKLQGEKNGLKVIIDMAKLKVPFQFNCTVATRK
                     KIRENPDEVRRVVWSLAEAGHFFKTRKEDALKIMQKYTRGLDRAVLEGAYGANYDFLV
                     EDTYPTLEGLKQTLEVQALTDPRAAKVKPEDFVDLRFVDEMKKSGFVDKLYGRK"
     gene            complement(1622..>2260)
                     /locus_tag="VFY96_10755"
     CDS             complement(1622..>2260)
                     /locus_tag="VFY96_10755"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5606986.1"
                     /translation="AAAYLLSRQVPPQRVRSDVEASATWWREIINVFDRHILLAAMLL
                     FTLHLSLPSLSSFVVLYAKQIGIGQFGWYYVVIGITSTLARPLLGRLSDKIGCGRSLL
                     VAFGFETAALLMMPLVKSLAGLMIAGAIWYVGSAIGGARILALAMERAPVERRGRAMA
                     SFSVAFPLSNGTGALLNGIVVDLAGYAWMYFTAAVLCSVGFALTAKNWSSLK"
BASE COUNT          522 a          626 c          620 g          492 t
ORIGIN      
        1 ccggcgaaaa gccgctgcca attgcgaaat ccagccggcc gccgcagaga ttgtcggcga
       61 ggccgtactc ttcggcgacg atcagcggat gacgaaacgg cagcacgctc accgccgggc
      121 caagccggat ttttttggtg cgctgcccca gagcggcgat gagcatcggc ggggaaacgc
      181 aggtgccgta ccagtaaaaa tgatgctcgc cgagccagaa agaatcgaag tccaactcct
      241 ccgcccattc cgccatggtc attacttctt cataatatct ctgattggaa cggccgagcc
      301 cgggatggtt atcgccgaaa acgaaaattc cgaacttcat tggtagtcct gaaccgtgaa
      361 atatccatag tgtacatcgg ttccccgagt ctcgcggtaa aggttgtcga gatcgtcata
      421 atgggcggcg atgccggcgt tgtttggaat gcagatggca ggttcaatca tggattactt
      481 cgcctaaatc cttaccgctc acccttacgt caggggtcag tggtgaggcg tgaggcgaca
      541 gttactttcg tccgtaaagt ttgtcgacga agccgctttt cttcatttca tcgacaaagc
      601 gaaggtcgac gaaatcttcg ggtttgacct tggcggcacg cgggtccgtg agcgcttgta
      661 cttccagcgt ttgtttcaaa ccttctagcg ttggataggt atcttcaacc agaaaatcgt
      721 aattggcgcc gtaggcgcct tcgagtacgg ctcggtcgag gccgcgcgtg tacttctgca
      781 taatttttag tgcgtcttct ttgcgcgttt taaaaaaatg gccggcttca gcgagggacc
      841 agacgacccg gcgcacttcg tcaggatttt ctctaatttt tttgcgcgtc gccacggtgc
      901 agttgaactg aaacggcact ttgagcttgg ccatatcgat aatcaccttg agcccattct
      961 tttcgccctg tagcttttcc ccctcgctga cgatggtgaa atccgcctgg ccgttgatgg
     1021 ttgcgcctag ccgcgccggc gcgccgccca aagtcacagc tttgatgtcc ttgtaagcga
     1081 taccgacctt gtttaaagcc aaacgcatcg cgaaatccgc cgaggtgccc gggatatgca
     1141 ccgcggcggt cttaccgcgc agctcttccg gcgatttgat gctggaattc ccaatgacga
     1201 aataagtcaa cgtgttcatc tggctttgaa caatcgcgat atccccaccg gcgagcttgg
     1261 cattgatgac ggcaggtccc actgcaccgg tgtaaccgag gtcgccagca agtaggctct
     1321 gaatcccacg cacgctgccg tcgataaaga tggtttcgaa gtcgaggcca tgctttttaa
     1381 aaatgccggc ttctttggcg atccagtaga cgaccgaaga gttgctggag tcggatatgt
     1441 acgcgatata gcttttcgcg tgggcagaat acgtgcttaa gctcagcgtc atcgacaagg
     1501 ctaaagcgat attcgaaaat attcgtcgtt tcattttcag accctcctct gcctagctgc
     1561 ggtgacagct cgcaacagca ttactccaag cgcgaaactt caacaagcgt cgctgcatcg
     1621 actacttcag gctcgaccaa ttttttgccg tcagggcgaa accaacggag caaagaaccg
     1681 ccgccgtgaa atacatccaa gcatagccag cgagatccac gacaatcccg ttcagcaatg
     1741 cgccggttcc attgctcaag ggaaacgcta cggaaaaact tgccatggct cggccacgcc
     1801 gttccaccgg cgcgcgttcc atggccaatg cgaggatacg ggcgccgcca atggcagagc
     1861 ccacatacca aatcgcgccc gcgatcatga ggccagcgag gcttttgacc aagggcatca
     1921 tgagtagcgc agcagtctcg aaaccaaagg caacgaggag cgatcggccg cagccgattt
     1981 tatcggatag acgaccaaga agcgggcggg caagcgtgct cgtgatgccg atcaccacgt
     2041 aataccaacc gaattggccg atgccgatct gcttggcgta aagcacgacg aagctcgaga
     2101 gcgagggaag ggaaaggtgc aaggtgaaga gcagcatcgc agccaagaga atgtgccggt
     2161 cgaaaacgtt gatgatttcg cgccaccacg ttgcgcttgc ctcgacatcg ctgcgaaccc
     2221 gttgtggcgg tacttgccgc gatagaagat aggctgcagc
//
LOCUS       DASULZ010000525         2753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_265207, whole genome shotgun sequence.
ACCESSION   DASULZ010000525 DASULZ010000000
VERSION     DASULZ010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265207"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..1271)
                     /locus_tag="VFY96_10760"
     CDS             complement(246..1271)
                     /locus_tag="VFY96_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5606987.1"
                     /translation="MGAKRKICVTGPMRIKLAETMLRDAGCELVLGKPQDDFREFRYL
                     RQDLVGLIGDCSIVYPSGRDMIGGDILDSCPDLQAVVKSSIGIETVDVDAATDLGILC
                     CNSPTVENYMGVAEATMGLIIALFKRLKLNETVIRNGGWKELHNRGTLMLGKTVGIIG
                     VGRIGQNVAKRLGAWGMKVLGYDPYVKQETVSSLGVKMVSFEELLQESDLVTVHVVLT
                     RETRGMLGMREFKMMKPTAFVVNTSRGQALKEAELVQALNENIIAAAALDVFEREPLP
                     LDSPLRQVDPLRLILTPHNIGANPGSAEAGQKMAAESMLAILDGRIPDTLVNPLAIPR
                     WKERFWS"
     gene            complement(1279..2301)
                     /locus_tag="VFY96_10765"
     CDS             complement(1279..2301)
                     /locus_tag="VFY96_10765"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5606988.1"
                     /translation="MANQFEVIDADGHITEEDNQLKEYMEAKYRDRAATLTPRDSWDR
                     SLSGKLGTRARDAKSWLEAMDKGGVSTAVLYPTNGLSVGWIREPDVAVAYCKAWNDFC
                     AEEFQKISPRLKCVALVPFQDVPEAVKELRRAINHSKLVGLMLPAVGLRLPLGHESFW
                     PIYEEAEKLDCMVGVHATVRGPHYFAGDMFDQFIEVHTLSHSFAQMMQACSMMFRGVF
                     DRFPKLRVAFMEAGCSWAPYWFGRMNEEWEKRGEVEAPMCKRKPTEYLKDGRIYFHAE
                     DYEPMIGATTEVITHKVIYYASDYPHWDTEFPENIEHLGRRKDLNDEAKRWLFADSAK
                     KLYNLH"
BASE COUNT          609 a          807 c          727 g          610 t
ORIGIN      
        1 acagaattgt gaactcgctg atcgccgcgt aggcagctga ccgagtaaat cgcgcaccgg
       61 aagtccgtgc ttctcgcaaa cctcaagcgg tggttcgaca ggactcacca cagcaaaagc
      121 cggaacttga cccccgatat atacattcgg gggtgacggg ttcagaaaaa tactgctaat
      181 agtgcagggg ggtgtgctag acagcacggc gctggattca acgaacctca gggacaccaa
      241 attacctatg accagaaacg ctctttccac cgtggtatcg ccagagggtt gacgagcgta
      301 tcgggaattc tgccgtcgag aattgccagc atgctttcgg cggccatttt ctgtcccgcc
      361 tccgccgatc cgggattggc gccaatattg tgcggcgtga ggatcaaccg gaggggatca
      421 acctggcgca acggactgtc aagcggcagc ggctcccgct cgaaaacatc gagggccgcg
      481 gcggcaataa tattttcatt cagagcttga accaactcgg cctctttcag agcctggccg
      541 cgcgaggtgt tcacaacaaa agcagtcggc ttcatcatct tgaattcgcg catgccaagc
      601 atgccgcggg tttcgcgggt caaaaccaca tgtaccgtga cgagatccga ttcctgcaac
      661 agctcttcga aagaaaccat tttgacgccg agtgaggaga cggtttcctg ctttacgtag
      721 gggtcgtatc caagtacctt catgccccag gcgccgagac gcttggcgac gttttgaccg
      781 atccggccga cgccgatgat tccgacggtt ttgccgagca tcaatgtgcc gcgattgtgc
      841 agctccttcc agccgccatt acggatgacg gtttcgttca atttcaaccg tttgaagagc
      901 gcgataatca atcccatcgt cgcctcggca acccccatgt agttctcgac tgtcggactg
      961 ttgcagcaga gaatacccaa atccgtcgcc gcatcgacat ccacagtctc gatgccgatg
     1021 ctcgatttga ccaccgcctg caggtcggga caggagtcga gaatgtcgcc gccgatcatg
     1081 tcgcggcccg agggatagac aatcgagcag tcgccgatca gtccaactaa atcctggcgc
     1141 agatagcgaa actcgcgaaa atcgtcttgc ggttttccga gcacgagctc gcaacccgcg
     1201 tcgcgcagca ttgtctctgc gagcttgatg cgcatgggtc cggtgacgca gatttttctt
     1261 ttcgcgccca tcggaacctc aatgcaagtt gtaaagtttc ttggcgctgt cagcgaagag
     1321 ccagcgcttg gcttcgtcgt tcaagtcttt gcgccgcccg agatgctcga tgttctcggg
     1381 gaactcggtg tcccagtgcg gatagtcgga cgcgtaatag atcactttgt gcgttatcac
     1441 ctccgtggtg gcgccgatca tcggttcgta atcttcggcg tgaaaataga tgcgaccgtc
     1501 tttcagatat tccgtcggct ttcttttgca catcggtgct tccacttcgc cgcgcttctc
     1561 ccactcttcg ttcatccggc cgaaccaata cggcgcccag ctacaacccg cttccataaa
     1621 ggcgacacgc agcttgggaa agcgatcgaa gacgccacga aacatcatgc tgcacgcctg
     1681 catcatttgc gcaaatgaat gggagagcgt gtgcacctcg atgaactgat cgaacatgtc
     1741 cccggcgaaa tagtgcggac cgcgcaccgt ggcgtggact ccgaccatgc agtcgagctt
     1801 ctcggcctcc tcatagattg gccagaagct ttcgtgcccg agcggcaaac gaagtcccac
     1861 ggctgggagc atcaagccaa ccagcttcga atggttaata gcgcggcgca gctccttcac
     1921 ggcctcgggc acatcttgaa atggcaccaa ggcgacgcat ttgagccgcg ggcttatctt
     1981 ttgaaactct tcggcacaga agtcattcca ggccttgcaa tatgcgaccg cgacgtcagg
     2041 ctcccggatc cagccgacgc tcaatccgtt ggtcggatag agcaccgctg tcgagacacc
     2101 ccctttgtcc atcgcctcca gccagctttt tgcgtcccgt gctctcgtgc ccaacttgcc
     2161 gcttaaagag cggtcccaac tatcgcgggg cgtcaatgtg gccgctcggt cacggtattt
     2221 cgcttccata tactccttga gctgattgtc ctcttcggta atatgcccat cggcatcgat
     2281 cacttcgaat tggtttgcca tagacgttcc tccctgactg gtctcgtctc cgtctacgcc
     2341 ccagagaagg tatgtgtcaa gcaccgttcg ggttcggaac gccgtttttc tattgtgaaa
     2401 tagaggcgta aaatggtaac cacacgaaac tagcgggagt aggacgggcc gccccgccaa
     2461 ccagtgcagc ttttttaacc gggatcgtct aacatgcatc ggcaaacgaa ggcgatatga
     2521 aaaaattgtg gcccataagc atcctcctac tatggaccct ttggaccaga acccaaacca
     2581 tgacctccga cagctggttg cctgcgccgg ggctcggttc ccaagagaaa tgccaggcaa
     2641 gtctcaagga caagctggac atgtggcgcc agtttaaaga cgctaaattc gaaggtaaca
     2701 ctgtgatctt taccagcaat aacagctcga tgacttacct ttgcttgcct gag
//
LOCUS       DASULZ010000526         3246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_63702, whole genome shotgun sequence.
ACCESSION   DASULZ010000526 DASULZ010000000
VERSION     DASULZ010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63702"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VFY96_10770"
     CDS             complement(<1..644)
                     /locus_tag="VFY96_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HEX5606989.1"
                     /translation="MQAFIDQLIQDGSFAGVPRELVYAIAMVLTALIVLSVFVAPLAG
                     VTSWLERRVWARMQSRIGPNRVGPQGVLQWLADGIKNLLKEDLIPAAADAKLFSLAPY
                     VVFMGFLCTFVVIPFGGSLIVADLNIGILYILAVTSLVVVGILMAGWASNNKWSLLGG
                     MRSAAQIVSYEIPAGLALLNVVFLAGTMSMQGIIKAQGWAPWDWFLFNNPFVFAA"
     gene            complement(665..1804)
                     /locus_tag="VFY96_10775"
     CDS             complement(665..1804)
                     /locus_tag="VFY96_10775"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052506.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX5606990.1"
                     /translation="MNDTAPAQTGFYTEDMTISVGPQHPSTHGVLRFVVKTDGEVISE
                     AIPDVGYLHRSIEKIGEKVTYHGYVPYTDRADYLAAMFANQAFCLAAERLAGTEVTRR
                     GEFCRVIACELNRIASHLISVGTFGQDIGAMTPFVHALRERETINNLMEEICGARLTY
                     NYIRIGGVGYDILPETCTRITAFLEHFEPIVDEFNRLLSGNKIFIERLAGVAVVSKED
                     AFSYNLVGPNLRGSGVKWDIRRDIPYSIYPELEFEVPIGTAHVGVLGDSFDRYWVRIR
                     EMQESCKIVRQCLSRMPKGPAIAKVPRKFRPPAGECYARVEAPRGDMGFYVVSDGSEY
                     PYRVRIRTGSYTAMSIIDKLSKGIMVADLIALIGSLDVDAPEIDR"
     gene            complement(1813..2319)
                     /locus_tag="VFY96_10780"
     CDS             complement(1813..2319)
                     /locus_tag="VFY96_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX5606991.1"
                     /translation="MDATEVYASLEKLFPGKVRDFKSEVREPYLFVEPAAIVEVCRFL
                     RNAPGFRFEVLSDLTALDWPKEEKIQMVYHLFSYSHNHQIVLKVDLPRDHPRIATLEN
                     VWKVANWFEREVYDLFGVIFEGHSDLRRIMLPEDWLGYPLRKDYVEQEEYDGISTQRA
                     PLVEKLLR"
     gene            complement(2322..2834)
                     /gene="nuoB"
                     /locus_tag="VFY96_10785"
     CDS             complement(2322..2834)
                     /gene="nuoB"
                     /locus_tag="VFY96_10785"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEX5606992.1"
                     /translation="MKPLINSLPETVFTTKVDDLLNWGRASSQWYMLFGLACCAIELM
                     QTGGPRADLDRFGAVPRATPRQSDLMIVAGTLTYKMAKRTKLLYDQMPDPKYVISMGS
                     CSNCGGLFQLAYSVCKGVDKIVPVDVYVPGCPPRPEALTEGLLRIQDKMMRERWLVKP
                     AKVADEQPSA"
     gene            complement(2854..3240)
                     /locus_tag="VFY96_10790"
     CDS             complement(2854..3240)
                     /locus_tag="VFY96_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX5606993.1"
                     /translation="MLFDFANVLVFTALGLGFVGVNLLIGKLLRPANPQARKLSTYEC
                     GEPAMGSAWVNFNIRFYVVALIFIIFEVEIAFIFPVATVFRRWVEGGAGVFALVEVLL
                     FIGILFLGLVYAWAKGDLEWVKKIRA"
BASE COUNT          794 a          949 c          828 g          675 t
ORIGIN      
        1 gcagcgaaaa caaatgggtt gttgaacaaa aaccaatccc atggtgccca accttgcgcc
       61 ttgatgatgc cttgcatgct catcgtgccc gcaagaaaga cgacgttcag cagcgccaaa
      121 cccgcgggaa tctcatagct cacgatctgg gctgcggagc gcatgccgcc caacagcgac
      181 catttgttgt tcgatgccca tccggccatc aggatcccga ccacgacgag agaggtgaca
      241 gccaaaatgt aaagaatgcc gatgttgagg tccgcaacga ttaaactgcc gccgaaggga
      301 atcacgacga atgtgcagag aaaccccatg aacacaacgt agggagcaag tgaaaacagc
      361 ttcgcatccg ccgcggcggg aattaaatct tctttgagga ggtttttgat tccatccgcg
      421 agccactgta ggactccctg cggaccgact cggttgggac cgatgcgcga ttgcatgcgc
      481 gcccaaaccc gccgctcgag ccagctggtg acaccagcaa gcggagcgac aaatacggaa
      541 agtacaatca aggcggtgag caccatcgcg atcgcataga cgagctctct tggtacgccg
      601 gcgaaggaac catcttgaat cagttgatcg ataaaagctt gcatgattat tcggcggtta
      661 tacgctaacg gtcgatttcc ggagcatcga catcgagact gccgatcagc gcgatcaaat
      721 ccgcgaccat aatacccttg ctcaatttgt cgataatgct catggccgta taggacccgg
      781 tgcgaattct cacacgatag gggtattcac tgccatcgct gacgacgtaa aatcccatat
      841 caccgcgcgg cgcttcgacg cgcgcgtaac attcgcctgc cggcggcctg aacttccgcg
      901 gtactttggc gatcgccggc cccttgggca tcctgctcaa gcactgccgt acgatcttgc
      961 agctctcctg catctcgcga atgcgcaccc agtagcggtc aaagctgtcg cccaacacgc
     1021 cgacgtgcgc cgttccgatc ggcacttcga attccaattc agggtaaatc gaatacggaa
     1081 tatcgcgccg aatatcccat ttcacccctg agccgcgcag attcggtcca acgagattat
     1141 aggaaaacgc atcttccttg ctcaccaccg ccaccccggc gagacgctcg atgaaaatct
     1201 tgtttcccga cagcagccga ttgaattcat cgacaatcgg ctcgaagtgt tcgagaaatg
     1261 ccgtgatccg cgtgcaggtc tccggcagaa tatcgtagcc gacgccgcca atgcggatgt
     1321 agttataggt caggcgcgcg ccgcagattt cttccatcaa gttattgatg gtttcccgct
     1381 cgcgcagggc gtggacgaac ggcgtcattg cgccgatatc ttggccaaat gtaccgaccg
     1441 aaatcagatg gcttgcgatc cggttgagtt cgcaggcgat cactcggcaa aattcgcccc
     1501 gtcgcgttac ttcagtaccg gcgagccttt ccgcagccag gcaaaacgcc tgatttgcga
     1561 acatcgccgc gagataatcg gcgcggtccg tgtaagggac gtacccatga taggtcactt
     1621 tctcgccgat tttttcgatg gagcgatgaa gatagccgac gtccggaatc gcttcgctga
     1681 tcacttcgcc gtcggttttg accacgaagc gcagcacgcc gtgcgtgctc gggtgctgcg
     1741 gccccacgct gatggtcata tcctcggtat agaaaccggt ctgtgcaggt gcggtatcat
     1801 tcatgatcga atttagcgca aaagtttttc caccaacggc gcccgttgcg tgctgatgcc
     1861 gtcgtattct tcctgctcga cgtagtcttt gcgcaacggg tagccgagcc aatcctcggg
     1921 caacatgatc cttcgtaaat cgctgtgccc ctcaaaaatc actccgaaca gatcgtaaac
     1981 ctcccgttca aaccaattcg caactttcca aacgttctcg agggtcgcaa ttctggggtg
     2041 gtcgcgcggc aaatcgacct tgagaacaat ttgatgatta tggctatacg aaaacagatg
     2101 gtagaccatc tgaatttttt cttctttagg ccaatcgagg gccgtcaaat cggagaggac
     2161 ctcgaagcga aatcccggcg cattgcgcag gaaacgacac acttccacta tggcagcagg
     2221 ctcgacgaag agataaggct cgcgcacctc ggatttaaag tcgcggactt ttccgggaaa
     2281 gagcttttcc agcgaggcgt atacttccgt ggcgtccatg cctacgccga aggctgctcg
     2341 tcagcgactt tggccggctt caccagccag cgctcgcgca tcattttgtc ctgaatcctt
     2401 aataagcctt cggtaagcgc ctccggacga ggcgggcaac cgggaacata aacgtctaca
     2461 ggcacgattt tatcgacgcc cttgcacacc gagtacgcca attggaatag tccgccgcaa
     2521 ttcgaacagc tgcccatgga aatcacgtat ttgggatcgg gcatctggtc gtatagcagc
     2581 ttcgtgcgct tcgccatctt gtaagtcagc gtccctgcga caatcatcaa atcggactgc
     2641 cggggagtcg cccgcggcac cgcgccaaac cggtccaggt cggcacgcgg accgccggtc
     2701 tgcatcaatt cgatggcgca gcatgccaag ccgaaaagca tgtaccactg ggacgacgct
     2761 cggccccagt tgaggaggtc atcgaccttc gtcgtaaaaa cggtttctgg taacgagttt
     2821 atcagcggtt tcatcaaaaa actcccgaac gaatcaggct ctgatctttt tgacccattc
     2881 taaatcgccc ttcgcccatg cgtacaccaa tccgagaaac agaatgccta tgaacagcag
     2941 aacttccacc agcgcgaaaa ccccagcgcc cccctccacc cagcggcgaa ataccgttgc
     3001 gacggggaag ataaaggcga tttccacttc aaagatgatg aaaatcaaag cgaccacata
     3061 gaagcggata ttgaaattga cccaggcact gcccatcgcc ggctcaccgc attcataggt
     3121 ggacaatttt cgcgcttgcg gatttgccgg gcggagcaat tttccgatca gcagattaac
     3181 cccaacaaat ccaagtccta atgccgtaaa gacaaggaca ttagcaaaat caaataacat
     3241 ccaaag
//
LOCUS       DASULZ010000527         3291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1179351, whole genome shotgun sequence.
ACCESSION   DASULZ010000527 DASULZ010000000
VERSION     DASULZ010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179351"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..1046
                     /locus_tag="VFY96_10795"
     CDS             45..1046
                     /locus_tag="VFY96_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HEX5606994.1"
                     /translation="MTKLSEMLSNIDMAAESDPLKPKVGISACLLGQKVRYDGGHKRD
                     FFLTEIFGRFVDWVPVCPEVEAGMGVPRESVRLVGTAPGVRMIAEKSGKDWTSAMRRF
                     AGLRSNELAPLNLSGYIFKKNSPSCGIEKVRVYSSSGMPARNGRGLFAAAVMSKFSLM
                     PVEEEGRLNDPALRENYIERVFAYARWQQTSSGAKSLGLLGQFHTVHKFLLLAHSERH
                     YRQLGRLVAEGKKQSLGSLYDQYGALLMQALAMRASVKKHANVLEHMVGYFSQQLSPS
                     ERAELVELIRDYRAQLVPLIAPITLIRHYVKKYAIDYLNQQVYLCPSPKELMLRNHV"
     gene            1208..1666
                     /locus_tag="VFY96_10800"
     CDS             1208..1666
                     /locus_tag="VFY96_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5606995.1"
                     /translation="MKTHRFETHIWLPHRGEQVFSFFADPINLNRLTPAWLHFQILTP
                     PAEIRQGTLLDYRLRLRGIPLRWQSEIEVWEPPSRFVDRQTRGPYSLWVHEHTFVEEN
                     GGTVVGDLVDYAVPGGSLVQKFFVAPDLRQIFQYRQSVLQEIFAAVAVAP"
     gene            complement(1702..3093)
                     /locus_tag="VFY96_10805"
     CDS             complement(1702..3093)
                     /locus_tag="VFY96_10805"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEX5606996.1"
                     /translation="MSTLDFLLADPRLTVRRSGAPDPDGRCVVYWMQRAQRATDNAAL
                     NAAIEAGNCLGKPVVVFFQLVPHSHHANLRHYQFLLEGLKDIATDLKKRRVGWIIRRY
                     PQHGLLRFCCEVRPCLVIGDENPMKEAELVKDKIARKIALPFWTVDADVIVPSRLLAR
                     EHYAARTIRPKIHALLPQFLQPVRNPAAKFTWHMPAQLLSLTANSSLLENLPIDRSVA
                     PSRHFAGGCGEARRCLERFLRSRLKGYASLRNKPDLDGTSQLSPYLHFGHIGALTIAL
                     AVQQANAPVKDRKAYLEELIVRRELAINFVRFNANYENFEACEPWADVTYRKHAHDRR
                     RYLYTERQLENAESHDPLWNAAQRQMMLTGWMHGYLRMYWAKKILEWTESPQVAYQIA
                     VRLNDRYELDGRDPNGYAGIAWAIVGKHDRAWGPERPVYGKIRYMSHESTSRKFDAKA
                     YITRIAALEKGGA"
     gene            complement(3090..>3291)
                     /locus_tag="VFY96_10810"
     CDS             complement(3090..>3291)
                     /locus_tag="VFY96_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HEX5606997.1"
                     /translation="PGRAWLEFEVEDRGSTSLIRQTAIFDPNGVGGLLYWYLLYPVHN
                     LMFAKMLRAIAARCRDESAMAK"
BASE COUNT          706 a          911 c          931 g          743 t
ORIGIN      
        1 actcttttcg tcttaacaaa aggatgaagt ggagtcaact atagttgaca aaattatcag
       61 aaatgttatc caatatagac atggcggccg aatccgatcc gctcaaacca aaggtgggaa
      121 tcagcgcctg tctcctcggc cagaaagttc gttacgacgg cggccataag cgcgatttct
      181 ttctcacgga aattttcggc cgctttgtcg attgggttcc cgtctgtcct gaggttgaag
      241 ccggtatggg ggtgccgcgt gagagcgtgc gcctcgtcgg aacagcaccg ggcgtccgaa
      301 tgatcgccga aaaatcgggt aaagattgga cctcggcgat gcgccgcttt gcggggctta
      361 ggagcaatga attggcgcca ctgaacttgt cgggctacat cttcaagaaa aattcgccaa
      421 gctgcggcat cgaaaaggta cgggtttaca gttcgagcgg catgccggca cggaacggcc
      481 gcggattatt tgcagcggcg gtgatgagca aattttcact gatgcctgtc gaagaggaag
      541 gccgtttgaa cgatccggct ctgcgcgaga attatatcga gcgggtcttc gcctatgccc
      601 gttggcaaca gacctcgtcg ggagcgaaat cgcttgggct cctcgggcaa tttcacacgg
      661 tgcacaaatt tttgctgctc gcccacagtg agcgccatta tagacagctg ggtcgcctgg
      721 tcgcggaggg aaaaaaacag tcgctcggaa gcctttacga ccaatacggc gctctgttga
      781 tgcaggctct cgccatgcgg gcgagcgtta agaagcacgc caatgttctc gaacatatgg
      841 tgggatactt ttctcagcag ctctcgccgt ccgagcgcgc cgaattggtt gagttgatcc
      901 gcgattatcg agcgcagctc gtgccgctga tcgcgccgat cacattgatc cgtcactacg
      961 tcaaaaagta cgctatcgat tatctgaatc agcaggtcta cttatgcccg agcccaaagg
     1021 agttgatgct gcgcaatcat gtctaggtct gggaactgaa ttgcattcaa tcatcgttcg
     1081 tttttttgcc gtcataccga aaccccacta tccagatgca ctcgcatttt ctaactccgg
     1141 tcgcgctatc cagaactagg tcggcgttgc cgggcacgac gcatgaatta tggaaccagc
     1201 ttcagacatg aagacgcacc gatttgagac gcacatttgg ctgccgcacc ggggcgaaca
     1261 ggtgttcagt tttttcgccg atccgattaa cctgaatcgg ctcaccccag cgtggctcca
     1321 ctttcaaatt cttacgccgc ccgcggaaat tcgccaagga accttgctcg attatcggct
     1381 ccgtctgcgc ggcatcccgc tgcgttggca gagcgagatc gaagtatggg aaccgccgag
     1441 ccgttttgtc gatcgacaga cgcggggccc ctattcgctt tgggtgcacg agcacacgtt
     1501 tgtcgaggaa aacggcggga ccgtcgtcgg cgatctcgtc gactacgccg tgccgggggg
     1561 atcgcttgtg cagaaatttt tcgtcgcgcc cgatctacgg cagatttttc aatatcggca
     1621 gtctgtgctc caggaaattt ttgcggctgt tgcggtagcg ccgtgaaatc aaaagaatgc
     1681 gtgtcgcgcg taatcgaaaa ctcaagctcc gcccttttcg agagcagcga tacgagtgat
     1741 atacgctttc gcatcgaatt tgcgcgaagt gctttcgtgg gacatatagc ggattttgcc
     1801 gtacaccgga cgctcgggtc cccaggcgcg gtcatgtttg ccgacgatcg cccaggcgat
     1861 gccggcgtag ccgttgggat ctcggccgtc cagctcgtaa cggtcattca gccgaaccgc
     1921 aatctgatag gctacttgcg gcgactccgt ccactcgaga atttttttcg cccaatacat
     1981 gcgcaggtat ccgtgcatcc aacccgtcag catcatttgc ctttgcgccg cgttccatag
     2041 cggatcatgg gattcggcgt tttccagctg tcgctccgta taaagatagc gccggcgatc
     2101 atgggcatgc ttgcgatacg tgacgtcggc ccagggctcg caggcttcga agttctcgta
     2161 gttggcgttg aagcgcacga agttgatcgc cagctcccgg cgcacgatga gttcttccaa
     2221 ataagccttg cggtctttga ccggcgcatt cgcctgttgt acggcaagcg cgatcgtgag
     2281 cgctccgata tggccgaaat ggagatacgg cgacaattgg ctcgtgccat cgagatccgg
     2341 cttgttacgc agtgaagcgt aacccttgag tcgcgagcgc aaaaaccttt cgaggcaacg
     2401 gcgcgcctca ccgcaaccgc ccgcgaagtg tcgcgacggc gctaccgagc ggtcaatggg
     2461 aaggttttcg agcaggcttg aattcgcggt caatgacaga agctgcgcgg gcatgtgcca
     2521 ggtaaacttc gccgcagggt ttcggacggg ttgcaagaat tgcggcaata acgcatgaat
     2581 cttcgggcgg atcgtccgcg ccgcataatg ctctctggca agaagcctgg acggcacaat
     2641 cacgtcagcg tccactgtcc aaaacggcag agcaattttc cgcgcgattt tgtctttgac
     2701 gagctccgcc tctttcatgg gattctcgtc gccgataacg agacaaggcc gtacctcgca
     2761 acaaaaccgt aaaagcccgt gttgcggata acggcggatg atccagccca ctcttctttt
     2821 ttttaggtcc gtggcgatgt ctttcaggcc ttcaaggaga aattgatagt gccggagatt
     2881 cgcatgatgc gaatgtggaa cgagttgaaa gaacaccaca accggcttgc ccagacaatt
     2941 gccggcttca atggccgcgt tgagcgcggc attatccgtc gctctctgcg cgcgctgcat
     3001 ccagtaaacc acgcatcgac cgtcggggtc gggcgcccct gaccgtcgaa cggttagccg
     3061 tggatcggcg agcaaaaaat ccagtgtgct catttggcca tggcgctctc gtcgcggcat
     3121 cgcgcggcga tggctcgcaa catttttgcg aacatcaaat tgtgcaccgg gtaaagcagg
     3181 taccagtaca ataagccgcc aacgccgttc gggtcgaaga tcgcggtttg gcgaatcagc
     3241 gacgtgctcc ctcgatcttc aacttcgaac tccagccagg cccgtcctgg t
//
LOCUS       DASULZ010000528         6554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1483163, whole genome shotgun sequence.
ACCESSION   DASULZ010000528 DASULZ010000000
VERSION     DASULZ010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6554
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483163"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="VFY96_10815"
     CDS             complement(<1..963)
                     /locus_tag="VFY96_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5606998.1"
                     /translation="MQKKNFSVIGKPLRKIDAMAKCVGETLYADDLNLPRMIHAKLLR
                     SPHPHARILGVNAARALKLDGVFAILTGEEMPEKFGIMPSTQDEEALVIDKARYAGDP
                     VAAVAAANEALAEKALGYIDVEYEVLKPILTIEDALAATEESERIHTWNRQANIQKAV
                     SFEFGDVEAAFAEADHIFEDTYFYQGNTHLPMEQYCAVGSYGPDNKLTVWSSTQTPHY
                     LHRALAKVLKLPASRIRVIATPVGGGFGGKSDPFSHEICAAKLSMITRRPVKITLTRE
                     ECFYVHRGRHPVKMLARTGVKNDGTIQAMQYKTYLDGGGYSSYGL"
     gene            complement(1310..1873)
                     /locus_tag="VFY96_10820"
     CDS             complement(1310..1873)
                     /locus_tag="VFY96_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5606999.1"
                     /translation="MSFYELNLRQQLKPPMSKKFDNKRPTKTLLRLRVNGEPHEVATE
                     INKTLLEVLREDMNLTGTKHGCELGECGTCAVLVAGKPVLSCLMLGIEAEHTEITTIE
                     GMMRDGKPHPLQNAFADLGAAQCGYCIPGILLTAKALLDENPRPTRDEIRQYLSGNLC
                     RCTGYTKIIEAVELAAKEMEGRANVSR"
     gene            complement(2009..3283)
                     /locus_tag="VFY96_10825"
     CDS             complement(2009..3283)
                     /locus_tag="VFY96_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HEX5607000.1"
                     /translation="MENIENSADRKKLQLERLHHMMRPVLATNGFYRRKFAGLDPDRL
                     NSLTEFRALPFTTKIELVQDQQDQPPFGSNLTDPLECYIRVHQTSGTTGKPMYWLDTK
                     ESWNWWASCWKLVFEAAGVTSRDRVYFAFSFGPFIGFWSGWEGARKLGALSISGGAQS
                     TAQRIHSIIDYGATVLVSTPTYALHMASEAKKAGINLAKDSAVKTTIHAGEPGASIPS
                     VKKLIEESWGAKCFDHAGATEVGAFGFQCASQPEAIHVNEHEFIAEIIDPRSGAAATE
                     GGQGELVITNLGRVGSPVIRYRTGDLVQPSREICPCARGFLLLRGGVLGRVDDMVVVR
                     GVNVFPSAVENVIREFSEIEEFRVEVYARQAMREIKLFIEPKSAEYSMTGLSERVIMR
                     MRERIGLRPEIELLSPGSLPRFELKAKRFFKL"
     gene            complement(3327..4241)
                     /locus_tag="VFY96_10830"
     CDS             complement(3327..4241)
                     /locus_tag="VFY96_10830"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5607001.1"
                     /translation="MKSLVAGILFVAVFASLPVITTRSDLLNLGVQIFLAVVLAQSWN
                     LLGGYAGQINLGHAAFFGLGALVTRTLWVGGTPLILALFPGALVAMLFGLIIGVPAFR
                     LRGAYFAIGTLGLAEILRITIGNLMPQISTISALELASYNILPRYYLTLGMAILCVVL
                     VALLIPSRVGLGIRAVREDEAAAEATGVSALRHKLLALALSTALSGLAGGAFAYYHVS
                     YYPQHTFSPAWTFDAVLMSYIGGVGTVHGPVLGALFYVILRELLAVKLVEFHLIIFGV
                     LFIFVVLFLPGGLIEAWRRIRRMIARST"
     gene            complement(4238..5110)
                     /locus_tag="VFY96_10835"
     CDS             complement(4238..5110)
                     /locus_tag="VFY96_10835"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5607002.1"
                     /translation="MILTQQLILGLLVGGLYGLAAAGLSLTFGVLRVLNVAHGELLML
                     GGYGTFWLFTLWKIDPFISLVAVAPAMFLLGLLLYWALFQFVVKAHEETRIKNSLLIG
                     FGLTLVLHALAIRLWTADERAVTTAYAGAVLRLGGLAVPWLRLTSLLFAFAILAALHL
                     FLAKARWGRAIRATAEDWEAAALMGIPVGKTYLLAFALGTALAGIAGGLVSVSYAISP
                     SIGLEWTLKALVVIVLAGLGSLFGSFAGGIILGLAEATGVVFLGGPYREVVGLILFLI
                     VLVVRPQGLFGARA"
     gene            complement(5194..5916)
                     /locus_tag="VFY96_10840"
     CDS             complement(5194..5916)
                     /locus_tag="VFY96_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010308119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5607003.1"
                     /translation="MLEVKNLQVAYGPLLALHAVDLIVHPGEMVALVGPNGAGKSTLL
                     KTISGLLTPRAGAIRWQGEAISAASPQRIVEHGIALVPEGRRLFAAMTVRENLELGAF
                     TERAQKEKRAQMERIFALFPRLFERQQQTAGLLSGGEQQMLALGRALMGLPRLLLLDE
                     PSLGLAPRIVESIFAVLVELHRDGMSVLIVEQNVYAVLELVQRAYILEGGRIVGEGEG
                     RKLLDNDHIRAAYLGPLARRAV"
     gene            complement(5942..>6554)
                     /locus_tag="VFY96_10845"
     CDS             complement(5942..>6554)
                     /locus_tag="VFY96_10845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5607004.1"
                     /translation="KTTLFNLVSGALAPDTGKIVFQGERIDGLPPHRLCHRGLARTFQ
                     LVRPFLGLTAEENVLVGLGYGRDRLTGSNARDKAGGLLTSVGLADRSDRPANTLTVMD
                     RKRLELARALATAPKLLLLDEFMAGLTPAETAVAINLLRQLQRSGLTVVLVEHIVWAL
                     LDLSHRIVVLSAGEKIAEGAPATVAKDPRVIEVYLGETVAGSP"
BASE COUNT         1479 a         1882 c         1861 g         1332 t
ORIGIN      
        1 gagaccatag ctgctgtacc cgccaccatc gagataggtt ttgtattgca tcgcctgaat
       61 cgtgccgtcg ttcttgacgc cggtcctggc gagcattttg actggatgac ggccgcggtg
      121 gacataaaag cattcttccc gtgtgagggt aattttcacc ggccttcgcg tgatcatcga
      181 tagctttgcg gcgcagatct cgtgcgagaa cggatcgctc ttgccgccga acccgccgcc
      241 gaccggcgtg gcaatgacac ggatccggct cgcgggcagc ttcaaaacct tggccaaagc
      301 gcgatgcagg tagtgcggcg tctgggtcga tgaccagacg gtgagcttgt tatccggacc
      361 gtaagagccg acggcgcaat attgttccat cggcagatgc gtgttgccct ggtaaaaata
      421 agtgtcctca aaaatgtgat cggcctcggc gaacgccgcc tcaacatcgc cgaattcgaa
      481 cgagacggct ttctgaatat tcgcttgtcg gttccaagta tgaattcgct cgctttcttc
      541 ggtcgcggcc agcgcgtcct cgatggtaag aatcggcttc agcacttcat attcaacatc
      601 gatgtagccc agcgcctttt ccgcaagcgc ttcgtttgcc gctgcgacgg cagcaacagg
      661 atcgccggcg taacgcgcct tgtcgattac caatgcttct tcgtcttgcg tcgacggcat
      721 gatgccgaac ttttccggca tttcttcgcc ggtaaggatt gcgaagacgc cgtccagctt
      781 gagcgcgcgc gcggcattga cgccgagaat gcgggcgtgc ggatgaggac tgcgcagcag
      841 ctttgcgtga atcatgcgcg gcagattgag atcatcggca tagagcgttt cgccgacaca
      901 cttcgccatc gcgtcaattt tgcgcagcgg cttgccaatg acagagaaat tcttcttttg
      961 cattatctaa tccacgtaat tgcgattctg ctttcgacgg tccgtccgcg cggttgcttg
     1021 ctggtcccat ttccgtcatt cccgcatgtc tttagcggga atcgaggcga attcggactg
     1081 gacccccgat taaaacattc ggggtgacga ctttgggata tcgtcgcgca catttcatcc
     1141 tttgtaatcg atcgtgagat catgaattac ttcgctctaa attttatgcg cctttgaccg
     1201 tcgcgctcta gcgttaccag cgagatcctt cgctgccgct caggatgaca gacaagtagg
     1261 tactcgctaa gtgtctttct gagccggacg cgaaaaatct gcgcttgggt taccgcgaaa
     1321 cattggctct gccttccatt tcctttgcag cgagctcgac ggcttcaatg atctttgtat
     1381 agcccgtgca acggcacaga ttgcccgaca ggtattgccg gatttcatcg cgtgtcggtc
     1441 tcggattctc atccaacagc gcttttgcag tgagaagaat tcccgggatg cagtagccgc
     1501 attgcgccgc gccgagatcg gcaaacgcgt tctgcaacgg atgcggcttc ccgtcccgca
     1561 tcattccttc gatcgttgtt atctcggtat gctccgcttc gatgccaagc atcagacaag
     1621 acagcaccgg cttacctgcg accagcacag cgcaggtacc gcactcgccg agctcgcaac
     1681 cgtgcttcgt gccggtcagg ttcatatcct cgcgcaagac ttccagcagg gttttgttga
     1741 tttcggtggc gacttcgtga ggttcgccgt tgacgcgaag acgcaagaga gtttttgttg
     1801 gccttttgtt atcgaatttc ttgctcatgg gcggcttgag ctgttgtctg aggttcaact
     1861 cataaaaact caaacgtcat tccggccaag ccggcgatag ctggcgcgag ccggaatcca
     1921 ggagtgtcgg ggtggtctgg attccccttt cgcagggatg acgaacagcg gcacggggtt
     1981 ctgtcacttg attttctcag acaaagcact agagcttaaa aaatcgtttc gccttgagct
     2041 caaaccgcgg caagcttccc ggtgaaagca gctcgatctc gggacggagg ccgatccgtt
     2101 cacgcatgcg catgatgact cgttcgctca agccggtcat cgagtactcc gcggactttg
     2161 gctcgatgaa aagtttgatc tcgcgcatcg cctgtcttgc ataaacttcg acacgaaact
     2221 cctcgatttc agaaaactcg cggatcacgt tttccaccgc gctcggaaaa acattgacgc
     2281 cccgcaccac caccatatca tccaccctcc ccaatacacc accgcgcaaa agcaagaacc
     2341 ccctcgcgca cggacaaatt tcacggctcg gttgcacgag atcgcccgtg cgataccgga
     2401 tcacggggct gcctactctg ccgagattcg tgatcaccag ctcgccttga ccgccctctg
     2461 tggctgccgc gccgctgcgc gggtcaatga tttcagcgat gaattcatgt tcgttgacat
     2521 ggatcgcttc cggttgagaa gcgcactgaa agccgaacgc gccaacctca gttgctccgg
     2581 catgatcgaa gcactttgcg ccccaggatt cttcgatgag tttcttgacg ctgggaatgc
     2641 tcgccccggg ttcgccggca tggatcgtgg tctttaccgc agaatccttg gcgagattga
     2701 tgccagcttt tttcgcttcg gacgccatgt ggagcgcgta ggtgggtgtc gaaacgagca
     2761 ccgtagcgcc atagtcgatg atcgagtgaa ttcgttgcgc cgtggactgc gcgccgccgg
     2821 aaattgacag tgcgcccaac ttacgtgcgc cctcccagcc tgaccaaaaa ccgatgaaag
     2881 gtccgaagga aaacgcgaag taaacccgat ccctcgaggt gacacctgcc gcttcgaaca
     2941 cgagcttcca gcaactcgcc caccaattcc aggattcctt cgtgtcgagc caatacatgg
     3001 gcttcccagt ggtgccggag gtctggtgaa ctcgtatgta gcattccaaa ggatcggtta
     3061 aattggagcc gaagggcggc tgatcctgtt gatcctgaac cagttcgatc ttggtcgtaa
     3121 acggcagagc tcgaaattct gttagcgaat tgagtcgatc cgggtcgagg ccagcgaatt
     3181 ttctgcggta gaagccgttc gtagctagca cgggacgcat catatgatgc aaccgctcga
     3241 gctgcagttt ttttcggtct gccgaatttt ctatgttttc cacggcgagc taaggctttt
     3301 cgtcttagat gcgacgttta ccaagcctaa gttgaacgag caatcatcct gcggatgcgc
     3361 cgccacgctt cgatgagacc gccggggaga aacaatacga cgaagatgaa gagaacaccg
     3421 aagatgatca gatggaactc tacgagtttt accgccaata gctcccttaa gatcacgtaa
     3481 aagagcgcgc cgagcacggg gccatgcacc gtgcccacgc cgccgatata ggacatcagc
     3541 accgcatcga aagtccacgc gggggaaaag gtgtgctgtg gatagtagct gacatgataa
     3601 taggcgaagg cgccgccggc caggccggac aatgccgtcg agagcgcgag agcgagaagc
     3661 ttatggcgaa gcgcactcac cccggttgct tccgcggcgg cttcgtcttc gcgcaccgcg
     3721 cgtattccaa gtccgacacg agagggaatc agcaaagcaa ccagcacaac acatagaatc
     3781 gccatcccga gagtcaaata gtagcgcggc aaaatgttgt aggacgcaag ctcgagcgcc
     3841 gagatcgtag aaatttgcgg catcagattg ccaatcgtga tgcgcaatat ctccgcgagt
     3901 cccaatgtgc caattgcgaa ataggcgccg cgcaggcgga aagccggtac gccgataatc
     3961 aaaccaaaca acatagccac gagcgcgccg ggaaaaaggg ccaagataag gggagtgccg
     4021 ccgacccaca gcgtacgcgt gaccagggcg ccgagtccga aaaatgccgc gtggccgaga
     4081 ttgatctgac cggcatagcc accgagcaga ttccaactct gcgccaggac gacggcgaga
     4141 aaaatttgca cgccaagatt caacaaatcg ctccgcgttg tgataaccgg cagtgacgcg
     4201 aataccgcga caaagaggat tcccgcgacg agcgatttca cgcgcgcgct ccgaagaggc
     4261 cttgcggccg caccaccaag acaatgagaa aaagaatcag cccgacgact tcgcggtacg
     4321 gaccgccgag aaacaccacg cccgtcgcct cggcgaggcc gaggatgatg ccgccggcga
     4381 agctgccgaa aagcgagccg agcccggcga gaacgatgac caccagcgcc ttaagcgtcc
     4441 attcgagacc gatggatgga cttatcgcgt aggagacgct gaccagtccg ccggcgatac
     4501 cggcaagcgc ggtgccaaga gcgaaagcga gaagataggt tttaccgaca gggattccca
     4561 tcaaggctgc cgcttcccaa tcttcggcgg tagcgcggat ggcgcggccc cagcgcgctt
     4621 tcgcaagaaa caggtgcagc gcggctagaa tcgcgaacgc gaaaagaaga ctcgtcaggc
     4681 gcagccatgg aaccgccaag ccgccgagtc gcagcaccgc accggcatag gcggttgtca
     4741 ccgcgcgctc atccgcggtc cacaatcgaa tcgcaagggc atgtaaaacc agagtcagac
     4801 cgaaaccgat cagcagagaa tttttgatgc gcgtctcttc gtgggcttta acgacgaact
     4861 gaaacagagc ccaatagagt aatagtccga gcaggaacat cgccggcgcg acggcgacca
     4921 aagaaataaa cggatcgatc ttccagagtg tgaacagcca aaacgtgcca tagccgccga
     4981 gcatgagcag ttcgccgtgg gccacgttca gaacgcggag cacgccgaat gtcaaagaca
     5041 gtccggcagc cgccagtcca tagaggccgc cgacaagcaa accgagaatg agttgttggg
     5101 tgagaatcat tgctgaaata cgaaaggcgt gaagggtaag agggtgaagt gtgttgccaa
     5161 cccctaactt ctcacgcctt acccgttact tcttcatacc gctcttcgcg cgagcgggcc
     5221 caagtaagcc gcacggatat gatcattatc gagcagtttt cggccctcac cttcgccgac
     5281 gattctgccc ccctcgagga tgtaggcgcg ttggaccaat tccagtaccg cgtatacgtt
     5341 ttgttcgaca atgagcacgc tcatgccgtc gcgatgcagt tcgacgagga cggcaaagat
     5401 cgactcgacg atcctcggcg ccagaccgag agacggctcg tcgagaagga gtagacgtgg
     5461 caagcccata agcgcgcggc cgagcgcgag catttgctgc tcgccgcccg agagtaatcc
     5521 cgcggtttgt tgctggcgct cgaaaaggcg cgggaagaga gcaaagatgc gctccatttg
     5581 agcgcgtttt tctttttgcg cgcgctcggt gaaagcaccg agctcgagat tttctcgtac
     5641 cgtcatcgcg gcaaaaagtc tgcgaccttc cgggacgagg gcgatgccgt gctcgacgat
     5701 ccgctgcggt gatgctgcgc tgatcgcttc accttgccaa cgaatcgcgc cggcgcgcgg
     5761 cgtaagcagg ccggaaatgg ttttgagcaa agtcgatttg cccgcgccgt tcgggccaac
     5821 cagagcgacc atttcgcccg ggtgaacgat tagatcgacc gcgtgtaggg cgagcagggg
     5881 accataggcg acttggagat tttttacttc gagcatggtt agctgaacac gttcaagtcg
     5941 ttcaaggcga accggctacg gtttcgccca ggtaaacctc gatcacgcgt ggatctttcg
     6001 ccacggtggc cggcgcgccc tcggcaattt tttcgccggc gctgagcacg acgatgcgat
     6061 gcgaaagatc gagtaacgcc cagacgatat gttccaccaa aacgaccgtc aaaccgctgc
     6121 gctgcaactg gcgcagaagg ttgatcgcta ctgccgtttc agccggtgtc agtccggcca
     6181 tgaattcatc gagcaacaaa agtttgggcg cggtcgcgag ggcgcgcgcc aactccaggc
     6241 gcttacgatc catcaccgtg agagtgttcg cgggacgatc cgaccggtcc gccaaaccga
     6301 ccgaagtcaa aagtccgccg gccttgtcgc gcgcgttgga tccggtaagc cgatctctgc
     6361 cgtagccgag accgacgaga acattctcct cggccgttaa gccgaggaag gggcgcacaa
     6421 gctgaaaagt tcgggctagg ccgcgatgac agagtcgatg aggtggtaaa ccgtcgatgc
     6481 gctcgccttg aaaaacaatt ttgccggtgt cgggagcaag cgcgccgcta acgagattaa
     6541 acagcgtggt cttt
//
LOCUS       DASULZ010000529         2222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_63900, whole genome shotgun sequence.
ACCESSION   DASULZ010000529 DASULZ010000000
VERSION     DASULZ010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63900"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..597)
                     /locus_tag="VFY96_10850"
     CDS             complement(121..597)
                     /locus_tag="VFY96_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5607005.1"
                     /translation="MALQLRRVVTGHDENGRAVVKIDENSKNLVSARPGSTACVVWTT
                     ESFPVDNTGAADEGLRKTGTTLSNGTVFRVVEFAPGVAPRVHRTDSIDYAVVISGEID
                     MELDDSVVHLKAGDVLVQRGTIHNWVNRGSMPCVIAFVLIDAKPVDVGGKVLHAQG"
     gene            complement(753..>2222)
                     /locus_tag="VFY96_10855"
     CDS             complement(753..>2222)
                     /locus_tag="VFY96_10855"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5607006.1"
                     /translation="GAGNMALGYARATKKPAVCLVSAGGGATNIVTPVAQAHKESVPL
                     FVISSDIDTAAAAKSPYSSFHGMEHVRLFQPITKQSAKLERIEELGSLLDSMFRQATR
                     GRQGPVFLIVPEDLQHAHLPEPLSFTPGAEPVAPAMDAAAARVAVDLLLQAKTPVVLA
                     GTGVDWAGARKELRELADHLSLPVAVSYSAKGVFPENDPLALGCLGFGSRPYARKFFQ
                     DADLILAIGATFSEGTTMRFGHEIIPEAAKIVQIDIDPDELGSNYPTALSIQADAKVA
                     LRAIIDEVRKRQSKPAGNGSRTEAIKQAKEAWKNDVERKPANNGLTYELVMHALNELL
                     TGNEILTTSGMTGDTLRGIDAVNPIIHAGEFRAIGTALATAMGVKLGRPEARVICVTG
                     DGSFMMEQQELATAAYHKIPIFVLILRNNAYGGMKRDQKKTYGGRIIGTELFIPDLPK
                     LADLYGARAFAVTKKDELKPIFQQALKTDDFVVVDVKLD"
BASE COUNT          474 a          649 c          610 g          489 t
ORIGIN      
        1 agcggtggtt cgactgtgct caccacaggc ggaattcgga ctggaccccc gattaacgta
       61 ttcgggggtg acggttttga gcgcttctat taaagctgca tacgatgcta gatcacccaa
      121 ttaaccttgc gcgtgcaaga cctttccgcc gacgtcaacc ggtttcgcat cgatcagcac
      181 gaaagcgatc acacacggca tactaccgcg gttgacccaa ttatggatcg tgccgcgctg
      241 caccagcaca tcgcccgcct ttagatgaac gaccgaatcg tcgagctcca tatcgatttc
      301 gccggagatg accactgcgt agtcgatcga gtctgttcga tgaacgcgcg gcgcgactcc
      361 aggagcgaac tcgacaacgc gaaatactgt gccgttactc aaggtcgttc cggttttgcg
      421 caacccctcg tcagccgcgc cggtgttgtc gacaggaaac gattcggtgg tccagacgac
      481 gcaagcggtc gaacccggcc gcgcagaaac gaggttcttt gaattctcat cgatcttcac
      541 gaccgcacgg ccattctcat catggccggt cacaacgcgt cgtaattgaa gcgccatttt
      601 atttcctcca agaaatggtc attccgggca accccggatt tcgtccgggg tagcgcgacc
      661 cggaatccag aaattcaaat acatctggat accggcttgg gccggtatgg cgaacaacac
      721 gtgtgatttg tcaatgcatt ggttctccgg ggctaatcca actttacgtc gacaaccaca
      781 aaatcatctg tcttgagtgc ttgctgaaaa attggcttca attcatcctt tttcgttact
      841 gcgaatgccc tcgcgccgta gagatcagcg agcttcggta ggtcgggaat aaaaagctcg
      901 gtgccgataa tgcgtccgcc gtaggtcttc ttttgatcgc gtttcatgcc gccgtaggca
      961 ttgttacgca gaatgagaac gaagattgga atcttatggt aagccgcagt ggcgagctcc
     1021 tgctgttcca tcatgaaaga accgtctccg gtgacacaga tcacacgcgc ttcaggccgg
     1081 ccgagtttca cgcccatggc ggtggccagc gcagtaccga tggcgcggaa ctcgccggcg
     1141 tggataatgg gattcactgc gtcgatgcca cgcaaagtat cgccggtcat acctgaggtg
     1201 gtcaggattt cgttgcccgt gagcaactca ttgagcgcgt gcatcaccaa ttcgtaagtc
     1261 aagccgttgt tggcgggttt acgttcgaca tcgttcttcc acgcttcttt tgcctgcttg
     1321 atcgcttctg ttctcgaacc attccccgcc ggcttcgact ggcgctttcg cacttcatcg
     1381 ataatcgcac gcaatgcaac ttttgcatca gcctggatcg acaacgccgt tggataatta
     1441 ctgccgagct catccgggtc gatatcgatt tgcacaatct tcgccgcctc aggaataatc
     1501 tcatggccga atcgcatcgt cgtgccttcg ctgaacgtcg ccccgattgc aagaatcaga
     1561 tcggcatctt gaaaaaattt acgcgcgtag ggccggctgc cgaaccccag gcagccgagc
     1621 gccagcggat cgttttcggg gaaaacacct ttggcgctgt agcttacggc cacgggcaac
     1681 gataaatggt cggctaactc acgcagctcc ttacgcgcgc cggcccagtc taccccggta
     1741 ccggccagta cgacgggagt tttcgcctgt agcaacaaat cgacggcgac gcgtgccgcg
     1801 gccgcgtcca tagcgggcgc gaccggctcc gctccgggcg taaagctcaa aggctcaggc
     1861 aaatgagcgt gctgcaaatc ctccggaaca ataagaaaca ccggtccctg ccgtccgcgc
     1921 gtggcttggc ggaacatgga gtcgagcaac gaaccgagct cctcgatgcg ctcgagcttc
     1981 gcgctctgct tcgtgatcgg ctgaaacagc cggacgtgct ccatcccatg aaacgaggag
     2041 tacgggcttt tggcggcagc ggcagtgtcg atgtccgagg agatcacaaa cagcggaacg
     2101 gactctttat gagcttgtgc gacgggtgtg acgatgttgg tggctccgcc gccggcggag
     2161 accagacaaa ccgccggctt tttggttgca cgggcatagc caagcgccat gtttcctgcg
     2221 cc
//
LOCUS       DASULZ010000530         3758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_468928, whole genome shotgun sequence.
ACCESSION   DASULZ010000530 DASULZ010000000
VERSION     DASULZ010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468928"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VFY96_10860"
     CDS             complement(<1..664)
                     /locus_tag="VFY96_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607007.1"
                     /translation="MPSQPKITLGLPDHPRCQAIIDGTVGIEGYELEVDPNFTSSGER
                     HYKYLHGQWDVGEVSAASLLRAIEKGQQLLAIPVFFERGPRQRNIYHCVDHLKHPSEL
                     KGKKFGCFRYGATAVVWARGYLLDAHNIKTTDMQWFVSGREVFIGHELPVKVQRIEPP
                     PQFGEEKLVLSKMLGTGELHGAVVPGDNGYAGFFGGGETPKMMAAYPGVKPLFENSQE
                     IID"
     gene            complement(811..1875)
                     /locus_tag="VFY96_10865"
     CDS             complement(811..1875)
                     /locus_tag="VFY96_10865"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5607008.1"
                     /translation="MKNGCCRIIIISFFCFWPCVVSAQTAEVKLSALEKVPAQERQQK
                     LYELAKGEGEAVIYANMDVSAMKPLTDGFMKRYPGTKATSVHFSGAAIITRLESEARA
                     GKPLADVVLSGQLGVLALIDKKIALRYRSPEREFFRAGLKDDDGLWTAYMTNVMVSAF
                     NTRNVKKDEAPRNVNDLLSPRWKGRLTMDSQSYVWFGTMLQYLGDEGGVRFMKQLNEQ
                     NISHQRGRRLMTQLVAAGEFDIAVETNLNSVLTLAKQGAPLSFAPIQPYFLSPSLVFM
                     SANAPHPYTGALFVDFLLSEEGQKIVVTTNRMPANSKVKSAESQILEGQDVRTPDIFD
                     IGRNYQAIGRRYQEIFTAAR"
     gene            complement(1900..2883)
                     /locus_tag="VFY96_10870"
     CDS             complement(1900..2883)
                     /locus_tag="VFY96_10870"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607009.1"
                     /translation="MSAIYRSLGTIALVTSLVLPWTDVGAELFRVALSTKDFGYLPLY
                     VGMRSGLFAQEGLEIQWIVVNSNVVVTALIAGEIDVAGIAGSSMRAAARGAPLKANFF
                     PYDKSLFVLMGAPGIKRVQDLKGKVIGTTGPGATTEIAASMVLQHYGMDPKKDVTFMT
                     IGGAETSLVAMRNGTIQARAFNPDAAFIMKKQGFTELGVLADMGPWPWAGYSTSDAHL
                     SNRRDKIRRWTRAMVKSLQLMVNRQEETYKIAQAEFGHPRDVTEGAANVCVKAIDPQN
                     PGGATDEAMRKNIELTITQPLKLSASPPLAQLVDFTLLREVHRELGIPGRK"
     gene            complement(2880..>3758)
                     /locus_tag="VFY96_10875"
     CDS             complement(2880..>3758)
                     /locus_tag="VFY96_10875"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX5607010.1"
                     /translation="FSFFNNVDLPPKSELHVAMGWRDSELGGTGPALLVGRKKYDFGF
                     GNPVGLSAMAYLGRGFYKNKIPLRAIGVFPTWDRLIFAVRKETGIESLEDLIQNKYPL
                     RISTRRRVKLQTTVYAIEQVLASYGMSFREIERWGGKIMEAPNPSGAERRDHIESSKA
                     DAVFDEGVKSWGQRALDAGMRFLPINDAAARHMQKIGFPNAALTLDHYPKLENPIRCV
                     DFSGWTFFCHADLAASVAYGMARAIDLCHEQIPVDHFDKRPMTMAEFCRGGEAGQLNI
                     PLHAGAKQYFREKGYL"
BASE COUNT          806 a         1049 c         1022 g          881 t
ORIGIN      
        1 aatcaataat ttcttggctg ttttcaaata gcggttttac gccagggtaa gccgccatca
       61 tcttgggcgt ttcgccgccg ccaaaaaacc cagcataacc attgtctccc ggaacgacgg
      121 cgccatggag ctcgccggta ccgagcattt tcgaaagcac gagcttttct tcgccgaact
      181 gtggtggcgg ctcgatgcgt tgcactttca ccggcagttc gtgtccgata aagacttcgc
      241 ggcccgaaac gaaccattgc atatcggttg tcttaatatt gtgcgcatcg agcaggtagc
      301 cgcgcgccca aaccaccgca gtcgcgccat aacggaagca gccgaacttt ttgcccttga
      361 gctccgacgg atgcttcaga tgatcgacgc aatgataaat gtttctctgg cgcgggccgc
      421 gctcgaaaaa aaccgggatc gcgaggagtt gctgaccttt ttcgatggcg cgaagtaagc
      481 tcgcggccga gacttctcct acgtcccatt gtccgtgcag gtacttgtag tgccgttcgc
      541 ctgagctggt aaagttcgga tcaacctcaa gctcatagcc ctcgatgccg actgtgccgt
      601 cgatgatggc ttggcagcga ggatggtcgg gaaggccgag ggtgattttg ggttggctgg
      661 gcataataaa ttcccttcga ggtgggaacg agtctagtta aaactttcaa gccgttcaaa
      721 aggttcaagc ggtcaatccc caggatgtag aggggacttg aacggagcgc agcgattgaa
      781 cgatttgaac ggcttgaacc aaagtttttc tcatcgcgct gcggtaaata tttcctgata
      841 ccgtctgccg atcgcctgat aatttctgcc tatatcgaaa atgtccggcg tacgcacgtc
      901 ctgaccctcg agaatctggc tctcggcgga tttgactttg ctgttggcag gcatgcggtt
      961 ggtggtgacg acaatcttct ggccttcttc ggacaggaga aagtccacaa ataacgcgcc
     1021 ggtgtacgga tgcggtgcat tagcgctcat gaataccaga ctggggctca agaagtaggg
     1081 ctgaatcggc gcgaaagaca acggcgctcc ctgtttggca agggtgagca cagaattcaa
     1141 gttggtttcg accgctatgt cgaactcgcc ggcggcgacg agttgcgtca tcagcctcct
     1201 gccgcgctga tgtgagatgt tctgctcgtt cagttgcttc ataaaacgca cgccgccttc
     1261 gtcgcctaga tattgcagca tggtgccgaa ccaaacgtag gactggctat ccatcgtcag
     1321 cctgcccttc caacgcgggc ttaaaagatc gttcacgttt cgcggcgctt cgtctttttt
     1381 gacgttccgc gtattgaacg cgctgaccat gacattggtc atgtaggctg tccacaagcc
     1441 gtcatcatct ttgagcccgg cacgaaaaaa ctcccgttcc ggcgaacgat agcggagcgc
     1501 aattttctta tcgatcaacg cgagcactcc caattggccg ctcaaaacca cgtcggctag
     1561 cggctttccc gctcgggctt cggattcgag gcgcgtgatg atcgcggcgc cggaaaaatg
     1621 cacgctcgtt gccttggttc ccggatagcg cttcataaaa ccgtcggtga gcggcttcat
     1681 cgcggaaaca tccatgttgg cgtagatcac cgcttcgcct tcgccttttg ccagctcgta
     1741 taatttttgt tgtcgctcct gcgccggtac tttctccagc gccgaaagtt tcacttcggc
     1801 ggtttgcgcc gaaactacgc agggccagaa acagaagaat gagataataa taattcggca
     1861 gcacccgttt ttcatggcga ccactctcca acggtctttt tactttctcc ccggaatgcc
     1921 aagttcccta tgaacttccc tcagcaaagt gaaatcgacc agctgagcca aaggcgggct
     1981 cgcgctaagt ttcaatggct gagtgatcgt gagctcgatg ttcttgcgca tcgcttcatc
     2041 cgtcgcgccg ccggggttct gtggatcgat ggctttaacg cacacgttcg ccgcaccctc
     2101 ggttacatcg cggggatggc cgaactcggc ttgcgcgatt ttgtaggttt cttcttggcg
     2161 attgaccatc agctgcaaac ttttcaccat cgcacgagtc caccgtctga tcttgtcgcg
     2221 ccgattactc aaatgcgcat ctgaagtcga atagcctgcc caaggccatg gtcccatgtc
     2281 ggctaacacg ccgagctctg tgaagccctg tttcttcatg atgaaagccg catccgggtt
     2341 gaacgcccgg gcttgaatgg tgccgttgcg catcgcgacg agggaggtct cggcgcctcc
     2401 gatggtcatg aacgtcacat cttttttcgg atccatgccg taatgctgca agaccatgct
     2461 agcggcaatc tcagttgtcg cgcccggtcc agtagtgccg atcactttcc ctttcaaatc
     2521 ttggactcgt ttgattccgg gcgcgcccat caagacaaaa agcgatttgt cgtaaggaaa
     2581 aaagtttgct ttcaaaggcg cgccgcgcgc tgcagcccgc atcgaagatc cggcgatgcc
     2641 ggccacgtcg atttctccgg cgatgagcgc cgtgaccacg acattgctgt tgaccacgat
     2701 ccactggatt tccaatccct cctgggcaaa caatcccgag cgcatgccga cgtatagcgg
     2761 cagataaccg aaatcttttg tcgaaagcgc gacgcggaaa agctccgctc caacgtcggt
     2821 ccatggtagc accaaagagg tgacgagagc gattgtgcct aacgatctat atatggcgct
     2881 cacaggtaac ccttttcgcg aaaatattgt tttgctccgg catgcagcgg aatattcaat
     2941 tggccggctt cgccgccgcg gcagaactcg gccatggtca tgggccgttt gtcgaaatga
     3001 tcgacgggaa tttgctcgtg gcaaagatcg atggctcggg ccatgccgta ggcgacggat
     3061 gcggccaaat cggcgtgaca aaaaaaagtc cagccgctga aatcgacaca gcggatgggg
     3121 ttttccaatt taggataatg gtccagtgtc agcgcagcgt tggggaaacc gattttttgc
     3181 atgtggcgcg cagccgcgtc gttgatcggc aagaagcgca tgcccgcatc cagcgcccgt
     3241 tggccccagc ttttcacacc ctcatcgaag acggcatcgg ctttgctgga ttcgatatga
     3301 tccctgcgct cggcgccgct cggattcggc gcttccataa ttttgccgcc ccagcgctcg
     3361 atctctcgaa agctcatccc ataagatgcg aggacttgtt cgatcgcata gacggtggtt
     3421 tgaagcttca ccctccggcg ggtcgagata cgcaacgggt atttattctg gatcagatct
     3481 tccagggact cgatgccagt ttctttgcgc acggcgaaaa tcagtcgatc ccaggtcgga
     3541 aataccccga tggcgcggag cggaattttg tttttataaa aaccgcggcc caggtaagcc
     3601 atcgccgaga ggccgacggg attgccgaag ccgaagtcat atttttttcg gcccacgagc
     3661 aacgccgggc cggtaccgcc gagctccgaa tcgcgccagc ccatcgcgac atggagctca
     3721 gatttgggtg gcagatcgac gttgttaaaa aaactgaa
//
LOCUS       DASULZ010000531         7471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_570831, whole genome shotgun sequence.
ACCESSION   DASULZ010000531 DASULZ010000000
VERSION     DASULZ010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7471
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570831"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1310
                     /locus_tag="VFY96_10880"
     CDS             <1..1310
                     /locus_tag="VFY96_10880"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX5607011.1"
                     /translation="FHDLKGQIGNDIELEIRKIYTRNPNNKKWFSKYRRLFTQRAEEV
                     LDDPEIDVVIEALGFQSAGQLGQFRDYILRAFANEKSVVTSDKAVLARHGKQIWAAAK
                     KAGRELRFEACVGGGIPVIRSLTESFAAEEPEAVFGIVNGTCNYILSQMERAGKPYAE
                     ALEEAQKKGYAETNPASDVNGSDAEAKLLLLSLVAFGLELQPGKTWRKGIEDIQAVDF
                     RYARRIGGSTIKQLAVAKRKGQAVEVYVAPALIPKDNFLAAVDGATNAVCFAGKSSGA
                     ARGERDSDYVLVGPGAGGGPTAVAVLGDVCELARGERRFGGVPSLVRAGTLKLQPQEQ
                     IAMPFYVRFVVKDRAGIVGDIGRTFGRIGVNISEIWQVRHVEDELHGLVENSLLKQKP
                     QEVLPFVMTLERTTLRQMRKALDSIRNRDYIVIDPVWFPIWGS"
     gene            1391..2611
                     /locus_tag="VFY96_10885"
     CDS             1391..2611
                     /locus_tag="VFY96_10885"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HEX5607012.1"
                     /translation="MKYIILQGDGMADYPLDVLGGKTPLEAARTPNMDWLARHGVYGI
                     AHVIPEGFPPGSDVGNMSIMGYDPAVYHTGRSPLEAASMGVSLGPDDIAFRCNLVTLG
                     GAADETYMEDFTSGHISTEEAAIVIRDLARELAGDGIEFFPGVSYRHLMVWRGGKEKM
                     MTTPPHDITDQKIAAYMPRGDGADRLLELMRQSQSILEGHPINQQRRAGEKRQANSIW
                     LWGQGRAPQLPSLTKRFGIKGGVISAVDIIHGLGVYAGLERIDVPGITGFLDTNYVGK
                     GEYGVRSLDKNDLVFIHVEATDEAGHMGDVEKKIQALEDFDEKVVGTVLQGMARRKDF
                     RILLMPDHPTAIALKTHVAEPVPFVLYSAAAPQDNGDLGYNEKDALKTGIVAKKAYRL
                     MEALIEGRATWTEI"
     gene            2596..3546
                     /gene="glpX"
                     /locus_tag="VFY96_10890"
     CDS             2596..3546
                     /gene="glpX"
                     /locus_tag="VFY96_10890"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014244182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX5607013.1"
                     /translation="MDRNLALEAVRVTEAAALNCARWTGRGDEKAADQAAVDAMRKAF
                     DALAIDGTVVIGEGERDEAPMLYIGEKVGSGGPKVDIALDPLEGTTICATGAPNALAV
                     IAMADGGNLLHCPDTYMAKIAAGPIGKGVIDLDKSPTENLHALAEAKRCNVEDLTVII
                     LSRPRHEAIIQEVRKAGARIRLIGDGDVSAAIATTKPETGIDLLLGIGGAPEGVLAAA
                     ALRCVGGEFQGRLAPRNNEEIERAKKMGVTDIKRKFQIDELAAGDVMFAATGVTDGDY
                     LHGVHFFPGGATTQSVVMRSKTKTVRVINASHYFEHKPNY"
     gene            3632..3835
                     /locus_tag="VFY96_10895"
     CDS             3632..3835
                     /locus_tag="VFY96_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607014.1"
                     /translation="MPLITWFIAFLIWFYWYDLTGTAEKRTERTRPVAKANPSDSPPA
                     KAPRERILEEDRKRLEDILKRGG"
     gene            4082..4561
                     /locus_tag="VFY96_10900"
     CDS             4082..4561
                     /locus_tag="VFY96_10900"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX5607015.1"
                     /translation="MDTYDSKTASRIVGVTLRQLQYWDEQDFIRPSVKLAEGRGTKRL
                     YSFHDLICLKVVKDLTRYGFNLQKIRRCLKPLREESSRTMRAPTSLKYLTDGEELFVI
                     TDDRRQILEAMERQFVLSLGIGSLVRELDTQARRMAPTLARKALKKTRGTDGHRTSA"
     gene            4568..7135
                     /gene="mutS"
                     /locus_tag="VFY96_10905"
     CDS             4568..7135
                     /gene="mutS"
                     /locus_tag="VFY96_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX5607016.1"
                     /translation="MEPAKITPMMRQYLQIKENYRDAILFFRLGDFYEMFFEDAHTAS
                     KILDIALTSRSKSEDGSVPLCGVPYHAADPYIQKLLDAGHKVAVCEQMEDPALAKGVV
                     KREVVRVITPGTVTAAEALEATTNNFLAGICAGLHGFGLALADITTGEFRCTEIIDEG
                     AFCDELGRIQPSELLLASSETRLRERLYKEYPLIHFTGIAEQLFTDSASGRLSARALA
                     HAEEYRNGLRAASAILAYLDANASESLKLIGELEFYRVSNSLLLDETTRSNLELITSH
                     RGDRNGSLLALIDCTRTAMGARRLRRWLLYPLLEPKEIIARHNAVQELVENFSVRQEL
                     KRGLGKVQDLERLAGRVVSGSALPKDLIAIKDTLSAVNEIRRQLEGFCAQLLTRLREG
                     LPEIPEVAALIERAIIDDPPFSLKQGGFIRAGFDRELDEIRDMRSHAKEWIRRFEAGE
                     RQRTGIGSLKVRYNRVFGYYIEVTNSHLKSVPQDYLRKQTLANGERFITPELKEYEAK
                     VLNSETLIEKLESALLDRVREQIAGFYPNLKSVSDALAVLDALLSLSEVAEARHFIRP
                     EIDSGLRLSIREGRHPVVEAAVGRGAFVPNDCFIDPDRSQMMLLTGPNMAGKSTYMRQ
                     IALIVILAQMGSFVPCAEARIGVVDRVFTRIGASDSLARGESTFMVEMKETANILHHA
                     TERSLILLDEVGRGTSTFDGISIAWAVAEALHDRECRPRTLFATHYHELTDLVQTKER
                     IKNYNFAVKEWRGDIIFLRSLMEGASSHSYGIHVARLAGLPTAVIERAKEILARLEGS
                     QATLETAGSDVPAQMALFGLGDDRLRQRLSALDASNMTPIEALNILHQLSEEAKKPQ"
     gene            7132..>7471
                     /locus_tag="VFY96_10910"
     CDS             7132..>7471
                     /locus_tag="VFY96_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607017.1"
                     /translation="MKQNVRRALVAELLMRLVAMILLLLPSGLGNAQRMALAAEAAPL
                     TSRAPASKVLVTGVRSTSSDGYARITVELSQDSKFEVRRLVEGKGTSPRVYVDIFGAQ
                     LALGSTRAVAV"
BASE COUNT         1659 a         2039 c         2212 g         1561 t
ORIGIN      
        1 tctttcacga cctcaaagga cagatcggta acgacatcga gctggaaatt cgtaaaatct
       61 acacgcgtaa tccgaacaac aagaaatggt tcagcaagta tcggcggctt tttactcagc
      121 gcgccgaaga ggtactcgat gatccggaaa tcgacgtggt tatcgaggca ttgggatttc
      181 aatccgcggg gcaactcgga caatttcgcg attacattct gcgcgccttc gccaacgaga
      241 aatctgtcgt cacctcggac aaggcggtgc ttgcgcgcca cggcaagcaa atttgggcgg
      301 cagcaaagaa agcgggccgc gagctgcgct tcgaggcttg cgttggcggc ggcatccccg
      361 tcatccgttc gctgaccgag agcttcgccg cggaagaacc ggaagcagtt ttcggcatcg
      421 tcaacggcac ttgtaattac attctttccc agatggaaag ggcagggaaa ccctacgccg
      481 aggcgctcga agaagcgcaa aagaaaggtt acgccgagac caatccggct tcggatgtga
      541 acggcagtga tgccgaagcg aaattgctct tattgagcct cgtcgcgttc ggcttggagc
      601 ttcagccggg aaagacctgg cgtaaaggca tcgaagacat tcaagcggtg gattttcgct
      661 atgcccgacg aatcggcggc agcacgatta aacagcttgc ggtggcaaag cgaaaggggc
      721 aggcggtcga agtttatgtc gcgccggccc ttatccccaa ggacaatttt ctcgctgcgg
      781 ttgacggcgc aaccaacgca gtgtgcttcg cgggtaagag cagcggcgct gcgcggggcg
      841 agcgtgatag cgattatgta ctggtcggtc cgggcgccgg cggcggtccg acggcggtcg
      901 ccgtactcgg cgatgtttgt gagttggcgc gcggcgagcg gcgctttggc ggcgtaccga
      961 gcctggttcg cgcgggtacg ctgaagcttc agccgcagga gcaaatcgcc atgccgtttt
     1021 acgtgcgctt tgtcgtcaaa gatcgcgccg gcatcgtcgg cgatatcggg cgaacctttg
     1081 gccggatcgg cgtcaacatc tcggagattt ggcaggtacg gcatgtcgag gatgagcttc
     1141 acggtttggt cgagaactcg cttctcaagc aaaaaccgca ggaagtcttg ccgttcgtca
     1201 tgaccctgga gcgaacgact ctgcgccaaa tgcgcaaggc tctggattcc atccgcaatc
     1261 gagattatat cgtaatagat cccgtctggt ttccgatctg gggatcatag taagctgcat
     1321 cgttcaaagt tccccgtccg ggttcaactt tgaaccttga acttggaaaa tcgagcggag
     1381 cgcaacttcg gtgaaataca tcattttaca gggcgatggc atggcggatt acccgctcga
     1441 cgtgctcggc ggcaagacgc cactcgaagc ggcgcgcacg cccaatatgg attggctggc
     1501 gcgccacgga gtctacggca tcgcgcatgt cattccggag ggatttccac ccggcagcga
     1561 cgtcggcaat atgagcatta tgggttatga cccggcagtt tatcataccg gccgctcacc
     1621 tctggaagct gcgagtatgg gcgtttcgct cgggcccgat gatatcgcgt ttcgctgtaa
     1681 tctcgtgacc cttggcggcg cggcagatga aacttacatg gaagacttca ccagcggcca
     1741 catctcgacg gaagaagccg ctatcgtcat ccgggatctc gcgcgcgaat tggcaggcga
     1801 tggcatcgaa ttttttcccg gtgtgagtta tcgccatttg atggtctggc gcggcggcaa
     1861 agaaaaaatg atgaccacgc cgccgcacga tatcaccgat cagaaaatcg ccgcgtacat
     1921 gccgcggggc gacggtgccg atcgtctgtt ggaattgatg cgacagtcgc aatcgattct
     1981 ggagggccac ccgattaacc aacaacgccg agccggagaa aagcgacagg cgaattcgat
     2041 ctggctctgg gggcagggta gagcgccaca gcttccatcg ctcacgaaac gctttggcat
     2101 caagggcggc gtcatttctg cggtcgatat tattcacggc ctgggagttt acgccggtct
     2161 agaacgaatt gatgtgccgg ggatcaccgg atttctcgac accaattacg tcggcaaggg
     2221 tgaatacgga gtgcgctcat tggataagaa tgatttggtt ttcatccacg tcgaagcgac
     2281 cgatgaagcc ggccatatgg gcgacgtcga aaaaaaaatt caggcgctgg aagacttcga
     2341 cgaaaaagtg gtcggcacgg ttttgcaggg aatggcgcgg cgcaaggatt tccgcatcct
     2401 gctgatgccg gatcatccca cggctattgc tctcaagacc cacgttgccg agcccgtgcc
     2461 gttcgttttg tattccgccg cagcaccgca agacaacggc gatttgggct acaacgaaaa
     2521 agatgcgctg aaaaccggga tcgtcgccaa aaaggcttac cggctgatgg aagcgttgat
     2581 cgaagggaga gcgacatgga cagaaattta gccctcgaag ctgtccgcgt taccgaagcg
     2641 gcggcattga attgcgcccg ctggaccggc cgcggcgacg aaaaagcggc cgatcaagct
     2701 gcggtggacg ccatgcgtaa agccttcgat gccctcgcga tcgacggcac agtggtcatt
     2761 ggcgaaggcg agcgcgacga ggcaccgatg ctctatatcg gtgaaaaggt cggttctggc
     2821 ggcccaaaag tcgatatcgc cctcgatcct ctcgaaggga ccacgatctg cgctaccgga
     2881 gcgcccaacg ctctcgcggt gatcgccatg gccgatggcg gcaacttgct tcactgtccg
     2941 gacacctaca tggcgaaaat cgctgcggga ccgatcggca aaggcgtcat cgatttggac
     3001 aagagcccga cagaaaatct tcatgctctc gccgaggcga agcgctgtaa cgtcgaagac
     3061 ctcacggtga ttattttatc ccgtcctcgg cacgaggcga ttatccagga agtcagaaag
     3121 gcgggggcgc gtattcgctt gatcggcgac ggcgacgttt ccgcagcgat tgcgaccacc
     3181 aagccggaaa ccggtatcga ccttttgttg ggcatcggcg gcgcccccga gggagtactc
     3241 gctgcggcgg cgctgcgttg tgtgggcggc gaatttcagg gccgattggc gccgcgcaac
     3301 aatgaagaga tcgaacgcgc caaaaagatg ggcgtgacgg atattaaaag aaaattccaa
     3361 atcgatgagt tggccgccgg cgacgtgatg tttgctgcca ccggcgtcac cgatggagat
     3421 tatttgcacg gcgtgcattt ttttcccggc ggcgcgacga cacagtcggt ggtgatgcga
     3481 tcgaaaacca aaaccgttcg cgtgatcaac gcgagccact atttcgagca caagcccaat
     3541 tactaaaccg cgctcacccc gacggcagag accgctcggc cgaaaccgca ctcagcggag
     3601 ctggctcaag attcttctga tctacacctg cgtgccgttg atcacctggt tcatcgcttt
     3661 tctgatttgg ttttactggt acgatctcac cggtactgcc gaaaagcgca ccgagcgaac
     3721 tcgacccgtc gccaaagcca acccgagcga ttcccccccc gcgaaagcgc caagagaaag
     3781 aatcctcgaa gaggatcgaa aaaggttgga agatattctc aaacgcggcg ggtgattata
     3841 ctgggcagtt gctggtgatg ctcggagcgg tcgttcaacg ctagcgcaac aatgacgagc
     3901 cccttgtgca cgacggcacc gcgcggtttc gtgattcgac gctctcgtca aagaaaaaac
     3961 tagggaattt ttttggcggt tgcaaccttc catttgaaga gcaccgatca agcttggttg
     4021 acaatcccgg gttcccagtc taccttcatg aaaacagcct tgttttcatc ctgcaaattt
     4081 cgtggacact tacgacagta aaacagcgag ccggattgtc ggcgtaaccc tgcgccagct
     4141 gcaatattgg gacgagcagg acttcatccg gccttcggtg aagttagccg aaggacgcgg
     4201 cacgaaacgc ctctattctt ttcatgatct catttgcctc aaagtcgtaa aggacctgac
     4261 acggtacggc ttcaatctgc aaaaaattcg ccgctgcctg aaaccgctca gagaagaatc
     4321 ttcccggacg atgcgcgcgc ccacctcgct caaatattta accgatgggg aagaattgtt
     4381 tgtgattacc gacgaccgcc ggcagattct cgaggcgatg gagcggcagt ttgtgctgtc
     4441 gctcggtatc ggcagcttgg tgcgagaatt agatacgcaa gcgcgccgta tggcgccgac
     4501 acttgcacgc aaggcgctca aaaaaacccg cgggaccgat ggccaccgca cctcggcttg
     4561 agaaaaaatg gaaccggcca aaatcacgcc gatgatgcgt cagtatctcc agatcaaaga
     4621 gaactaccgc gacgccattc tgttctttcg tctgggtgat ttctacgaaa tgtttttcga
     4681 agacgcacat acggcctcaa aaattctcga tattgctttg acctcgcgca gcaaaagcga
     4741 agacggctct gtgccgttat gcggcgtgcc gtatcatgct gccgacccat atattcaaaa
     4801 actgctcgac gccggccata aagtggcggt ttgcgagcag atggaagacc cagcgttggc
     4861 gaaaggcgtg gtcaaaagag aagtcgtgcg cgtcattacg ccggggacgg taaccgctgc
     4921 cgaggctctc gaggctacca cgaataattt tctcgccggc atttgcgccg gcctgcacgg
     4981 cttcggtctc gccttggcag atattaccac cggcgaattt cgttgcacgg aaattatcga
     5041 cgagggtgcg ttttgcgacg agcttgggcg gattcagccg agcgagctgt tgttggccag
     5101 ttccgaaact cggcttcggg agcgcctcta caaagaatat ccgctcattc acttcaccgg
     5161 catcgccgaa cagctgttta ccgattccgc cagcgggcgt ctatcagcgc gtgctctggc
     5221 gcatgcggag gagtatcgga atggccttcg agcggcatcc gccattttgg cttatctcga
     5281 cgccaatgcc tcggagtctc tcaagctcat cggggaactc gagttctaca gagtttccaa
     5341 ctctctcctg ttggatgaaa cgacccgatc aaatttagaa ctgatcacca gccatcgcgg
     5401 ggatcggaac ggctcgcttt tggcgctgat cgattgtact cggaccgcca tgggggcacg
     5461 ccggttacgc cgctggctct tgtatccgct gttagagccc aaggaaatta ttgcccgtca
     5521 taacgcggtt caagagctcg tggaaaattt ttccgtacgc caggaactca agaggggatt
     5581 gggaaaggtt caggatttag agcgactcgc cgggcgggtc gtatcgggca gcgctctgcc
     5641 taaggatttg atagccatca aagacacgct ctcggcggtg aacgaaatac gtcggcagct
     5701 cgagggcttt tgcgcgcagc tgctgacccg gttgcgtgaa gggcttccag agattccgga
     5761 ggtggcggca ctgatcgaac gcgcgattat cgatgatccg cctttttctc tcaaacaggg
     5821 cggttttatt cgcgcgggtt tcgatcgtga gctggatgaa attcgcgaca tgcgctccca
     5881 tgccaaagaa tggattcggc ggtttgaagc gggcgagcgt caaaggaccg gaattgggtc
     5941 gctgaaggtc cgctacaatc gcgtcttcgg ttattatatt gaagtcacca actcacacct
     6001 caaatcggtg ccgcaggact atttacgcaa gcagacgctg gctaatgggg agcggtttat
     6061 cacgccggag ctcaaagaat acgaagccaa ggtgctcaac tcggaaactc tgatcgaaaa
     6121 actcgaaagt gcgctgctcg accgagtgcg cgagcaaatc gccgggttct atccgaattt
     6181 gaagagcgtg agcgacgcgc tggcggtctt ggatgctttg ctatcgcttt cggaggtcgc
     6241 tgaagcgcgt cattttatcc ggcccgagat cgatagcggg ctccggttgt cgatccgtga
     6301 agggcgccac ccggtcgtgg aagcagccgt cggtcggggt gcttttgtgc ccaacgactg
     6361 ctttatcgat cccgaccgat cgcagatgat gcttctcaca ggtccgaata tggcgggcaa
     6421 gtcgacctat atgcgccaga tcgcactgat cgtgattctc gcgcaaatgg gcagctttgt
     6481 gccatgcgcg gaggcgcgta tcggggtcgt cgatcgcgtg tttactcgaa tcggcgcttc
     6541 ggactcactc gcgaggggag agtccacttt catggtggaa atgaaagaga ctgccaacat
     6601 tctccaccac gccaccgagc ggagtctgat tttgctcgat gaagtcggac gcggtaccag
     6661 cacgttcgac gggatctcca ttgcatgggc ggtggccgag gcgttacacg acagagagtg
     6721 ccggccgcgc acacttttcg ccacccacta ccacgagctt acggatctgg tgcagacgaa
     6781 ggagcgcatc aagaattaca attttgcggt gaaggaatgg cgcggcgata ttatttttct
     6841 gcgcagtctc atggaaggcg cctcgagtca cagctacggt attcacgtcg ctcgtctagc
     6901 ggggctgccg actgcagtga tcgagcgcgc caaagaaatt ctcgctcgcc tggaagggag
     6961 tcaggccacg ctcgaaaccg cgggaagtga tgtgccggcg caaatggctc tgttcggttt
     7021 gggcgatgac cggctgcgtc agcgcttgag cgcgctcgac gcctcgaaca tgacgccgat
     7081 tgaagcgctc aatattctgc atcagttgag cgaagaggca aagaaacctc agtgaagcaa
     7141 aacgtcagac gcgccctggt tgccgaactc ctcatgcggc tcgtcgccat gatcttgctg
     7201 cttctgccgt cgggtttggg gaatgcgcaa cgaatggctt tggccgccga agccgcgccg
     7261 ctgacttctc gcgcgccggc tagtaaagtt ctcgtgaccg gcgtgcgttc cacgtcctcc
     7321 gacgggtatg cacggatcac ggtcgagttg tctcaggaca gcaaatttga agtgcgccga
     7381 ttggtagaag gcaaagggac ttcgccacga gtctacgtcg atatcttcgg cgcccaactc
     7441 gcgctcggct caacgcgggc tgtcgcggtc g
//
LOCUS       DASULZ010000532         6599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1280537, whole genome shotgun sequence.
ACCESSION   DASULZ010000532 DASULZ010000000
VERSION     DASULZ010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6599
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280537"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..1090
                     /locus_tag="VFY96_10915"
     CDS             35..1090
                     /locus_tag="VFY96_10915"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5607018.1"
                     /translation="MKLSIASLCLALTICAPGSTKRVLAQQNPAKRNFQAEIDAAIQS
                     AKTAAGFEHLGTLVRTCLLPQSGGENLTDNVPGYVADPASAPRRETWYADPAKIFDNL
                     YFVGGKLHSSWALTSKEGIILIDTIYPYNSEPLILGGMQKLGLDPKNIKYVLISHAHA
                     DHIGGAELLQTRYKARIVMGGPDWEWVEKYPNRYKTMAPKRDIVATDGMKISLGGSTV
                     TVWLTPGHTPGTMSYTFTVLDGGKPVNVAYSGGTAFNFVNNTPDPGIKNFQTYIDSQK
                     HMAEKAAAAQATVLLSNHSEFDNAVNKNRMLAGRGQGPHPYEIGADSLQRYFQVMQGC
                     ARAAQLRLEQKAAEGMP"
     gene            complement(1335..2204)
                     /locus_tag="VFY96_10920"
     CDS             complement(1335..2204)
                     /locus_tag="VFY96_10920"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607019.1"
                     /translation="MNPLKIGIGVPSIGRFPPYIAEAGGLFEREGLNVEVINQKDEEK
                     AVPDIVAGHTPIGTPNAPSLIFSVLDGSDIVLVGGLLNRPAFYLAVDPTITTIAELRG
                     KRVGINQPRRMAGMTMLALLKKWRFSAETDLKLVDLGINDRSLEALIEKQIDAALLPP
                     EKAFLAEAQGFKVVADSLQLDFHWVPLATTRRFLRDNRDVVTKVCAIYRESIRLFKTE
                     PKETIAEITRRLPALADKPQVIKKCYRVFADLLEPSLTPSPAAITAILEEVGLQDPRA
                     KEIDPMSIIETVD"
     gene            complement(2291..3727)
                     /locus_tag="VFY96_10925"
     CDS             complement(2291..3727)
                     /locus_tag="VFY96_10925"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5607020.1"
                     /translation="MTSKAADIVFKNGRVVTPGGTLEGGAAILDGKIAVVGRDAFLPA
                     ADREIDLAGKYLMPGAIDPECHLGSHRPLADDFQSETRAAAAAGVTTWGMQLVSLAIT
                     GGADKVKGPADIPSFTQAVPLYYEASRSAAIDYFLTPILGTDEHVAEIPYLAREHGIS
                     SFKLHLQMQGPWKSSWPAYSFDDGSIYNVFATVAKLGAPAIALLHCENWEIARVLQER
                     LIAEGRTDMGAWDDRSPAFTEAGHARTYLYYARMAACPIYIVHATTAETFAEIEKARA
                     EGLTVYSEIGTHYLVLHKDAWKINVPLRDRSTHETLWRALASGAIDCIGSDHVAHTRT
                     RESMETGNVWTTISGFPSRVEGMLPMMLSEGVNKGRISIERLAQVASENPARIFGLYP
                     RKGVIVPGADADLIVVDLQRRATITKQLLHTITPWSIYEGRDVTGWPVMTLVRGNIVM
                     EWPEGESRAKITEGSVGQYIKRSLAKQR"
     gene            complement(3752..4732)
                     /locus_tag="VFY96_10930"
     CDS             complement(3752..4732)
                     /locus_tag="VFY96_10930"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607021.1"
                     /translation="MHRLLIRLACMILLVFSIAIAHAQQSSKLTPINYGNIVLTASTW
                     PFLIAEQEGFFVKESIDFKRVIGGNTTATTQALVAGSTDIAQMNLVNLLGANSAGADL
                     VVIAGDSTVPIYTLIVHPSIKSYADLKGKRLAVTGPTDPLNYIFARMLAANGLTPADY
                     ELIGLGGAPQRLAAVQNGGVAGALVNQPSDFIALASGFNSLGLSTDYVDNFQYTITGA
                     RRDWAQKNRPVVVRFLRAYLKACEFFYDPKNKEAAVRALAERTKSDKEEAEKTYALYI
                     KTKKTIPRDGGVDLQGARKVAESWKDFGLPKAPAPVDGVIDLSYLAEARK"
     gene            complement(4805..5800)
                     /locus_tag="VFY96_10935"
     CDS             complement(4805..5800)
                     /locus_tag="VFY96_10935"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607022.1"
                     /translation="MRTSNTVRFSLFLLLLGYCIGGARENFAADKLRISYSAVNATQA
                     FLWVAQEKGIFAKHGLEGELLYINSGTMNIAALVGGSVQIAGGGPVSIEARLRGIKLT
                     ILGNPLPWLASNLVAHPDIKAIPDLAGKFAGISRFGSSTDQGFRYLFRKNGLNVDRDL
                     KMLQMGADSSRVAALKAGTIQYTFLGAAATDNARALGFRVLGTAQQMAIPFPWTSVVV
                     EESWLNKNRELAYRYMKCGTEAIVTLKRNRVDSERIIGKYMKITDPKLIATEFDFVAA
                     LMPDYIAPTMDGIKLILENFGKEYPDAPRRDPKEFVDGSIIERLKSERFVEGLKF"
     gene            5926..>6599
                     /locus_tag="VFY96_10940"
     CDS             5926..>6599
                     /locus_tag="VFY96_10940"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607023.1"
                     /translation="MADWRNRVLIFFLIMLGFSACGGAQDKKLYTLSLGYSAISGSFA
                     PLWVGFDQGLFSKHGIDLKMAYIQGNRVMLSALTAGEIQLYQGGAEGLIRLVSGGGDG
                     IFVGTQYNFVGHYVLMTDPSITRLEELRGKRIALDPTSPTYGYMLKVLEQVGLKKEDV
                     SFVHFGTAGQPERAMAVLRKQAAATILTAPNTYAAEKQGLRKFSVIRELGIRQLITVT
                     ATTKKYL"
BASE COUNT         1436 a         1798 c         1862 g         1503 t
ORIGIN      
        1 agatcgcgaa atcaggtgtt catgtggagg tctgatgaag ttatcaatag ctagtttgtg
       61 tcttgcgctc acgatttgtg ctccgggtag caccaaaagg gtgttggcgc agcagaatcc
      121 agctaagcga aattttcaag ccgaaataga cgcagcgatc cagtcggcga aaacggcggc
      181 gggattcgaa catctcggga cactggtgcg tacctgttta ctaccgcaga gcggcgggga
      241 aaatttgact gacaacgtac caggttatgt cgccgacccc gccagcgcgc cgcggcgcga
      301 aacctggtat gccgatccgg ccaagatctt tgataaccta tacttcgtcg ggggcaagct
      361 tcattcgtcc tgggcgctga cgagcaagga aggaatcatt ctgatcgata cgatttatcc
      421 ttacaattcc gagccgctga tcttgggcgg catgcaaaag ctcggtctcg acccaaaaaa
      481 tatcaaatac gttttgatct cccacgccca tgctgatcac atcggcggag ccgagctgct
      541 ccagacgcgt tacaaagcgc ggatcgtcat gggcgggccc gattgggaat gggtggagaa
      601 atatccgaat cgttacaaaa ccatggcgcc gaagagagat atcgtcgcta cggatggcat
      661 gaagatctcg ctcggcggta gtacggtgac tgtctggctg acgcccggcc atacgccagg
      721 aacgatgtcc tatacattca ctgtgctcga tggcggcaag ccggttaacg tggcgtattc
      781 gggcggcacc gctttcaact ttgtgaacaa caccccggac ccggggatca aaaattttca
      841 gacctacatc gattcacaaa agcacatggc ggaaaaggcc gcagcggcgc aggcgaccgt
      901 tctgctctcc aatcattccg agttcgacaa tgccgtgaat aagaatcgaa tgctcgccgg
      961 ccgtggccag ggtccgcatc cttacgaaat cggcgccgac tcccttcagc gctactttca
     1021 agtcatgcaa ggttgtgcac gggcggcgca actgcggtta gagcaaaaag cggccgaagg
     1081 catgccgtag actttgcgag tcggcgagaa atctcgaagc aacatagccc gaatggagtt
     1141 tagcgcaatc cgggttcttg gatttccttt cacttcatcc aggctatacg attctggacg
     1201 caaacccgcc gcttgtcatt ttcgaccgta gcgagaaatc tttccgggga tggatctctc
     1261 accgacttta tcctgcgtct gaccgaaggg ttcgagatga cactcgcgcg ctgttggcga
     1321 gcaagcaggt tgcattagtc gacggtttct atgattgaca ttggatcaat ttctttggct
     1381 cgtggatctt gtaggccgac ttcttccaag atggcagtaa tcgcagcggg tgacggcgtg
     1441 agggacggtt ctaagagatc cgcgaaaact ctgtaacatt tcttgatcac ttgcggtttg
     1501 tccgcgaggg ctggaagacg gcgggttatt tcggcgatag tttccttggg ttcggttttg
     1561 aagagccgga tactttcacg gtaaattgca cagacctttg taactacgtc gcgattgtca
     1621 cgcaaaaacc gccgcgtggt cgcgagcggt acccaatgga aatccaactg aaggctatct
     1681 gcaacaactt taaatccttg cgcctccgcc aaaaatgctt tctccggcgg taagagcgcg
     1741 gcatcgatct gcttctcgat cagtgcttca aggctccggt cgttgatacc gagatcgacg
     1801 agcttgagat cggtttcagc tgaaaagcgc cactttttta atagcgcgag catggtcatg
     1861 ccggccatgc ggcgcggctg attgattccc acgcgttttc ctctgagctc tgcaatcgtg
     1921 gtgattgtcg gatcgacagc aagataaaat gccggacggt tgagcagtcc gccaacgagg
     1981 acgatatcac taccgtcgag caccgagaag attaatgatg gtgcgttcgg cgtgccgatc
     2041 ggcgtgtggc cggcgacgat atcgggaacc gctttttctt cgtccttttg attgatgact
     2101 tccacgttca aaccttctcg ctcaaaaagt cccccggcct ctgcgatgta cggaggaaag
     2161 cggccgatac tgggaacgcc aatgccgatt ttgagcggat tcatggatac tctatctgat
     2221 gagttgccgg atacaattct gcgttgctac gcaacaatta agaattcggt caaacagact
     2281 tatgctgggc tcaacgctgc ttcgctagag agcgttttat atattgccca acggagcctt
     2341 cggtaatttt tgcccgcgat tcgccctcgg gccattccat gacgatattg ccgcgaacca
     2401 acgtcatcac cggccatccc gtaacatccc gtccttcata gatcgaccac ggcgtgatcg
     2461 tgtggagaag ctgcttcgtg attgtcgcgc gccgttgcaa atcgacgacg atcaggtcgg
     2521 catcggctcc gggaacgatc acccctttcc gcgggtaaag tccgaaaatg cgcgcgggat
     2581 tttcgctcgc gacctgtgcc agtcgctcga tcgagatacg gcctttgttg actccttcgc
     2641 tcagcatcat cggcagcatg ccctcgactc tcgaaggaaa gccgctgatc gtcgtccaaa
     2701 catttcccgt ttccatcgac tcgcgggttc gggtgtgggc gacgtgatcg ctgccgatgc
     2761 agtcgatggc gcctgacgcg agggcgcgcc aaagcgtttc atgagtcgag cgatctcgta
     2821 gcggcacgtt gatcttccac gcgtccttgt gcagcaccag ataatgcgtg ccgatctcgc
     2881 tataaacagt caggccctcg gctctggctt tctcgatctc tgcaaacgtt tccgcggtgg
     2941 tggcgtgaac gatataaatc ggacaggccg ccatgcgggc gtagtaaaga taggttctcg
     3001 cgtgtccggc ctcggtgaat gccggcgagc gatcgtccca cgctcccata tccgtccgtc
     3061 cctcggcaat cagacgctct tgaaggacgc gagctatttc ccaattctcg caatgcagta
     3121 aagcaatggc gggcgcgccg agcttcgcca cggtggcgaa gacattataa atgctaccgt
     3181 cgtcgaatga gtaagccggc caggaagatt tccatggccc ttgcatttgc aggtgcaact
     3241 tgaaggagct gatgccatgc tctctggcga ggtaagggat ctcggcgaca tgctcgtcgg
     3301 tgccgagaat cggcgtcagg aaataatcga tcgccgcgct gcgcgatgcc tcatagtaaa
     3361 gcggtaccgc ttgagtaaat gagggaatat ctgccggtcc cttgacctta tccgcgccgc
     3421 cggtaattgc caggctgacc agctgcattc cccaggtcgt cacgcccgcc gccgcggccg
     3481 ctctggtttc ggactggaag tcgtcggcga gcggtctatg actgcctaaa tggcactccg
     3541 gatcgatagc gccgggcatc aggtatttgc cggcgagatc gatttcacgg tcggcagcgg
     3601 gcaagaatgc gtccctgccg acgacggcga ttttgccgtc gaggatcgca gcgccgcctt
     3661 ctaaggtgcc gcccggcgtg acgacccgac cgttcttaaa aacaatgtcg gcagctttgc
     3721 tagtcatttt tgtcatccat ttgtttttga cttactttct tgcctcagcc agataactca
     3781 agtcgatgac gccatcgacc ggtgcaggcg cctttggcag gccgaaatct ttccagctct
     3841 ccgcaacctt gcgcgccccc tgcagatcga caccgccgtc gcgcggaatc gtctttttgg
     3901 tctttatata gagggcatag gtcttttccg cttcctcttt gtccgacttg gtgcgctcgg
     3961 cgagcgcacg tacagcagct tctttgtttt tcggatcgta aaaaaactcg caggctttca
     4021 gataggcgcg cagaaagcgc accacaaccg ggcggttctt ttgcgcccaa tcgcgccgcg
     4081 ctccggttat cgtgtactgg aaattgtcca cgtaatcggt cgagagaccc agcgagttga
     4141 atcccgacgc aagcgcgatg aagtccgagg gttgattgac taacgcgccc gctactccgc
     4201 cattctgcac cgcggcgagt cgctggggcg cgccgccgag accgatcaat tcataatcag
     4261 cgggtgtcag gccgttggcg gccaacatgc gggcgaaaat ataattcagc gggtcggtgg
     4321 gtccggttac cgcgagccgt ttccctttca agtcagcata gcttttgatc gacggatgaa
     4381 cgatcaaagt atagatcggc acagtcgagt ccccggcgat gacgaccaga tcggctcccg
     4441 ccgaattggc gccgagcaga ttcaccagat tcatttgagc gatatctgtc gaaccggcta
     4501 ccagtgcctg tgtggtagca gtagtgttgc cgccgatgac gcgcttgaaa tcgatgctct
     4561 ctttgacaaa gaacccttcc tgctcggcga tcaaaaaagg ccatgtggac gccgtcagaa
     4621 cgatgttgcc gtagttgatc ggcgtgagtt ttgagctttg ttgcgcgtga gcgattgcga
     4681 tcgaaaaaac aagcaggatc atgcatgcca gtcgtatgag aagacgatgc atcgtcggac
     4741 ctcctctcgt cgtcaatgat tgaagcgttg cagaaccagg cgtatttcaa aacgcaaaac
     4801 cacactagaa cttcagtccc tcgacaaatc gctcgctttt gagccgctcg atgatcgaac
     4861 cgtcgacgaa ttccttcggg tcgcgtcgcg gcgcgtccgg atattctttg ccaaagttct
     4921 cgaggatcag cttgatgccg tccatggtcg gcgcgatgta atccggcatc agcgcggcga
     4981 cgaaatcaaa ttccgtggcg attaatttgg gatcggtgat tttcatgtat ttgccgatga
     5041 tgcgctcgct atcgacgcgg ttccgcttca gagtcacgat cgcttcggta ccgcacttca
     5101 tatagcgata ggcgagctcg cgattcttgt tcaaccaact ctcttccacc accacggacg
     5161 tccaaggaaa cgggatggcc atctgctgcg cggtgccgag cacgcgaaaa ccgagcgcgc
     5221 gggcgttatc ggtcgcggcg gcgccaagga aggtatactg aattgtgccc gccttaaggg
     5281 cggcgacgcg actagaatcg gcgcccatct gcagcatttt cagatcgcga tccacgttca
     5341 gtccgttttt ccggaacagg tagcgaaatc cttggtcggt agacgaaccg aaacgcgaga
     5401 tccccgcgaa tttgccggcc agatcgggaa tcgctttaat gtccggatgc gccacaagat
     5461 tggacgcaag ccacggcagc gggttgccga gaatggtgag cttgattcct ctcagacgcg
     5521 cttcgatcga taccggcccg ccgccggcga tttgcacgct gccgccgacc aacgcggcaa
     5581 tattcatggt gccgctgttg atgtagagca attcgccttc gaggccgtgc ttggcgaaaa
     5641 tgcctttctc ctgcgcgacc caaagaaatg cttgtgtggc attgaccgct gaatacgaaa
     5701 tgcgcagctt gtcggcggca aaattttctc gggcgccacc gatacagtaa cccaacaaca
     5761 gcaggaacag gctgaagcga acagtgttcg acgttctcat gcgattgtgg atacagggat
     5821 ggctcgccgc ggtcaagcgg tcttctgggc cgtgaaaact ctgggtcgcc cttgggattt
     5881 gcttccgatg cggataaagt tctggcgaat cctttcgagg tggctatggc cgattggcga
     5941 aatcgtgtgc tgattttttt tctgatcatg ctcggatttt cggcttgcgg cggggcgcag
     6001 gacaaaaagt tatataccct cagcctcggc tactcggcga tcagcggcag ctttgcgccg
     6061 ctctgggtgg gattcgatca aggcctattc agcaagcatg gcatcgattt gaaaatggcc
     6121 tacattcagg gcaatcgagt catgctctcg gcattgactg ccggtgaaat ccagctttat
     6181 caagggggag ccgaaggcct gatccgactc gtgtcgggcg gaggtgacgg tatatttgtg
     6241 gggactcagt ataatttcgt cggacattat gtgctgatga cggatccgag catcacgcga
     6301 ctcgaagagc tccgtggtaa gaggatcgcg ctcgatccaa ccagtccgac ttacggttac
     6361 atgctaaaag tattggagca ggttggtctc aagaaggaag acgtgagctt tgtgcatttc
     6421 ggtacggcag gacagcccga aagagcgatg gcggtcctgc gcaaacaggc agccgcgacg
     6481 atcctcacgg caccgaatac ttacgcagcc gaaaagcaag ggctgagaaa attttccgtg
     6541 atccgcgagc tgggcatccg tcaactgatc acggttacgg cgacgacaaa gaaatatct
//
LOCUS       DASULZ010000533         2522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1280559, whole genome shotgun sequence.
ACCESSION   DASULZ010000533 DASULZ010000000
VERSION     DASULZ010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280559"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2522
                     /locus_tag="VFY96_10945"
     CDS             <1..>2522
                     /locus_tag="VFY96_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5607024.1"
                     /translation="PNLPLDLHGRTQNPRIALPLAKHALTAGLSLGDLRISEALRLRA
                     RGQGVVLARDGADNPLLYVLEQGKLRLVLLAFDLMASDLPLRIAFPILFHNLLEWFHP
                     ERLEFPGEAVQAGDPFALTLARGDDNVEITLPSGKKESLSTTSSRLLFAETLQAGIYS
                     FKTASRAGRFAVNLCDEDESQIVPRAQLTSAPGKSVEAARESESGAFSLWPALLIAVL
                     LLLAIELILALRQKLPIYPIIVRGAALALLTLALFDPRIYRSVMALDVILGIDLSRSV
                     GQEGRETALEILEAAKRLAGPDTRTGLLSFGRTPQWEWLPHPGIVTAELSSRLDRDET
                     DIQAALQAAVAQVGEGREGKILLISDGNENHGEVSRVIPLLRAQGVEVWTLPVSLARG
                     RNEVYLSDLTVPRQVDSAEGFEIHGTVESARAAPARIRLLRDGVLQAEREVQLRSGSN
                     QVSFRDTLTERGNHAYELLVDSPDDTLAENNLLRGVVTVKGPPRVLLLSTEKSSQRAI
                     ARVLEVQGYAVVQAAPDHHSLSLSEISAYDLLVLDNVPAFQLSHAKMENIEKYVRDLG
                     GGLLVIGGSQSYGAGGYFRTPLERVLPVDMRPPARLEMPHVALLFVLDKSGSMGAGGE
                     GSTKLDLAKAAAIAAADIMNPTDQVGILAFDASWDWALPFRQVGRGDWISDRLASLQS
                     DGGTDLYKAMLEAYRGIAAKPAAIKHVIVLSDGLTDKADFHSLIGRMARDGITVSTVS
                     VGSDADVQLMADIAKDGKGRGYVALDPETIPQIFTTETLLISRDLIIEKPVTPTMIAG
                     VGPLKGLPPSNIPALGGYVLTYPKPRSELLMRVDKDPLLVAW"
BASE COUNT          507 a          697 c          770 g          548 t
ORIGIN      
        1 tccaaactta cccttggacc tccacggcag aacgcaaaat ccacgcatcg ctttaccgct
       61 ggcgaagcat gcgttgaccg ctggcttgag cctcggtgat ctaagaatca gcgaagcgct
      121 acggctgcga gcgaggggtc aaggcgttgt gctagcgcgc gatggcgcag acaatccgct
      181 actctacgtt ttggaacaag gaaagctccg cttggtcctg ctagccttcg atctcatggc
      241 atccgatctg ccgctgcgga tcgcttttcc aatcctgttt cataatctcc ttgaatggtt
      301 tcatcccgag cggctcgaat ttcccggaga agcggtgcag gccggtgatc cgtttgcgct
      361 gacgctggcg cgcggcgacg ataatgtgga gatcactttg ccgtcaggaa aaaaagaaag
      421 tctctcgaca acttcgagcc ggctgctgtt tgccgagaca ttgcaggcgg gaatttacag
      481 ctttaaaacc gccagccgtg ccggtcggtt cgcagtgaac ctttgtgacg aagacgaatc
      541 gcagatcgtc ccccgggcgc aactcaccag cgcgcccggc aaatcggtcg aagcggcacg
      601 tgagtccgag agcggcgcgt tttcgctgtg gccagctctg ttgatcgcgg tgctgctact
      661 actcgcgatc gaactaattt tggccttgcg gcaaaaatta cccatctatc cgataattgt
      721 tcgcggtgca gctctggcac tcctgacgct cgcactgttc gatccgcgga tctatcggtc
      781 ggtgatggcg ctggatgtga tccttggtat cgatctatcg cgcagcgtgg gacaggaagg
      841 acgcgagact gccctagaaa ttctcgaagc ggcgaagcgg ctcgccggac cggacacgcg
      901 caccggcttg ttgagttttg gccgcacgcc gcaatgggag tggttgccgc acccgggaat
      961 tgtcacggcg gagcttagtt cgcgactcga ccgcgacgag actgatattc aggccgcgct
     1021 tcaggctgcc gtggcccagg ttggcgaagg gcgtgagggc aaaattctcc tgatctcgga
     1081 tggcaatgaa aatcacggcg aagtttcgcg tgtgattcca ctgctgcgcg cccagggtgt
     1141 cgaggtctgg acgcttccag tgagtctggc gcgtggccgc aatgaagtgt acttgagcga
     1201 tttgacggtg ccccggcaag tcgatagcgc cgaagggttc gaaatccacg gcaccgtcga
     1261 aagcgcccgt gcagcccccg cgcgcattcg tttgttgcgc gacggcgttc tacaggcgga
     1321 gcgagaagtg cagctcaggt ctggaagtaa tcaagtgagt tttcgtgaca cactcaccga
     1381 gcgcggcaat catgcctacg aactgctggt ggattcaccg gatgacacgc tggccgaaaa
     1441 taatctgctc cggggtgtgg tgacggtgaa agggccgcct cgggtgcttt tgctttcaac
     1501 ggaaaagtcg agccagcgtg cgattgcgcg agtattagaa gtccagggtt atgcggtggt
     1561 tcaagcggcg ccggatcatc actcgctcag tttatccgag atctccgctt acgatctcct
     1621 ggtgctcgat aatgtcccag cgtttcaact ctcgcacgcc aaaatggaaa acattgaaaa
     1681 atatgtgcgc gatctcggcg ggggattgct cgtcatcggc ggatcccaga gttacggcgc
     1741 gggcggatat ttccgcacac cgctcgaacg ggttcttcct gtagatatgc gcccacccgc
     1801 ccggctcgag atgccgcatg tggcattgct cttcgtgctc gacaaatcgg gcagcatggg
     1861 tgccggtggt gaaggcagca ccaagctcga tctggccaaa gcggctgcca ttgcggctgc
     1921 cgacatcatg aaccctacgg atcaagtggg tattttggct ttcgatgcga gctgggactg
     1981 ggcgctgcct tttcgtcaag tcggaagggg tgactggatt tccgatcgtc tcgcttcgct
     2041 gcagtccgac ggcggtaccg atctatacaa agcgatgctt gaagcatacc gcggtattgc
     2101 cgccaagccg gcggcgatta agcatgtgat cgtgctttcc gatgggctga cggataaggc
     2161 ggatttccat tcgctgatcg gccgcatggc gcgcgacggt atcacggttt cgacggtatc
     2221 ggtgggcagc gatgccgacg tgcagctcat ggccgatatc gcgaaggacg gtaaaggccg
     2281 cggctatgtg gcactggatc ccgagaccat tccgcaaatt tttaccactg aaacgttgct
     2341 catttcgcga gacctgatca tcgaaaagcc ggtgacgcca actatgattg cgggggtcgg
     2401 gccgctcaaa ggattaccgc ccagcaatat acctgccttg gggggatacg tcctcactta
     2461 tcccaaaccg aggagcgaat tgctgatgcg tgtggataaa gatcccctcc tggtggcgtg
     2521 gc
//
LOCUS       DASULZ010000534         4460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1076746, whole genome shotgun sequence.
ACCESSION   DASULZ010000534 DASULZ010000000
VERSION     DASULZ010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076746"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..1316)
                     /locus_tag="VFY96_10950"
     CDS             complement(216..1316)
                     /locus_tag="VFY96_10950"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX5607025.1"
                     /translation="MAAALFSGSAKLEITAVPKLKPAPLRDFCAALLAAGGMPGADAG
                     FVAELLVKAELRGYAGHGVTRVGQYLGFIRNKTYDLSARPQIEREGKITAVIDGHHYV
                     GQIAARMAVELAIKKAREHGAGIVCVRRAGHTGRLADYMEMATTQGLIGMGAVSVGSA
                     TTTLYGGMKPITGTNPMAFGIPARNGKSIILDFATASMSMGEIQKRVAKNEAIPDGVL
                     LDGHGEPTTEFKKFRGPPRGVFLPFGGYKGSGVALVTEILGGLLSGNGLGRDWWKSGG
                     HGVNGVFLQAFSVEEFQPLEVFYDRVDEFVDFIKSTPLAPGFSEILLPGEAGRRREEN
                     HQNQGVELDDETWAELVDLASELGVGNIPPLA"
     gene            complement(1280..2155)
                     /locus_tag="VFY96_10955"
     CDS             complement(1280..2155)
                     /locus_tag="VFY96_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5607026.1"
                     /translation="MAEKISFQSDGLKISGLIDTPPDYKPGERRPAFIVLHGFGGNKD
                     GQGQAVVAKQFAQWGYVTLRIDFRGCGESEGEHGRIICLDQVADTRNAISYLTTRPEV
                     APDKIALVGSSFGAAVAVFTGGADRRVAAVISCGGWGNGERKFRGQHPTPEAWAKFTQ
                     MLEDGKRHRERTGKSLMVPRYDIVPIPEKLRNRMSSGSIMEFPAETAQSMNDFRADDQ
                     VGNISPRPLLLLHSANDSVTPTYESIEMFKRARQPAELHLLADVDHFTFNEENPRLTN
                     IVHEWLQRYFPVRPS"
     gene            complement(2293..3513)
                     /locus_tag="VFY96_10960"
     CDS             complement(2293..3513)
                     /locus_tag="VFY96_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005183786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5607027.1"
                     /translation="MSEVAAKFDVGGVLLKQPFKIRRLGHFGFNLSNMEDGVRFYVDL
                     LGFRISDVMDYSRRAKDPAEVAGLGDPNGYFTRYGTDHHAMVLFPKRVRDALGRQERP
                     GITVNQITWQVGSLAEVGAAIKWFNQRDIKQQRSGRDMPGSNWHTYLYDPDGQSNELY
                     YGIEQIGWNGHSKPRAMYDRGFDKPPELPQISEFQEVEEARANGVDIFSGYRYLEKLP
                     ANYDVDGILLPRPFKIVRLGPVYLFSENMAAAVEFYRDTLGFTLTEEVSWQGERCFFF
                     RCNTEHHSLALFPLELRDAFGLSPHSKCASFGIQLANYRQLKDAVKFLRENGIPVMQS
                     IPAELHPGIEYSATVRDPDGHTIQLYCAMEQIGWDGKPRPKELRRPRSLSEWPESLDA
                     ADAYLGEPFLGPWG"
     gene            3686..>4460
                     /locus_tag="VFY96_10965"
     CDS             3686..>4460
                     /locus_tag="VFY96_10965"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5607028.1"
                     /translation="MPLQELHANGKVQWPGEARIAVFLSFDFQGGEDVKPDKNGHINH
                     EEYTQGEYGPNTGIWRILRILSSHEVRATFLTCGGIAERYPEALAAIAAQGHELAGHG
                     YHHEVARDLSKEEERDVIKRTTDMILNRAQKRPFGWRSCTQSPNSLELLLEQGYLWNS
                     NSFAHDLPFVWQNGKKKLVELPRQPFGDGRTYGHRDSGNPNDTLVVWKAMFDQFYEES
                     ALAPTFCPFQFHPYISSRPGRAKTLSEIIAYMKSHKGVWF"
BASE COUNT          989 a         1254 c         1230 g          987 t
ORIGIN      
        1 accatggttt gataggggcg cagcttggag aaacgatcgt tgccccagtg cgcggcaaac
       61 tgcgccgcga gctccgcgtg atcttcctgc aaggccacgt agcaacggcc atctttcagt
      121 tcacgaatta ccataccttg cctcctaaca tcagtggggt atttcgcacg cccgggacaa
      181 ttcgacttta ttcatttacc caggggctgc gcaagtcaag caagtggcgg tatattgcct
      241 accccgagct cgctcgcgag atcaacaagc tccgcccacg tttcgtcgtc cagttccacg
      301 ccctggttct ggtgattctc ttctcgccgc ctgcccgctt cacccggtag gagaatttcg
      361 ctgaaaccgg gcgccagcgg tgtcgatttg atgaaatcga caaattcgtc gacgcgatca
      421 tagaaaactt cgagcggctg aaactcctcg acggagaacg cctgaagaaa aacgccgttg
      481 acgccgtgcc caccgctctt ccaccagtct cttcccagac cattacccga gagcagaccg
      541 ccgagaatct cggtgacgag agccacgcca gagcccttgt agccaccgaa aggcaaaaac
      601 accccgcgcg gcggaccgcg gaatttttta aattctgtag tcggttcgcc atgaccgtcg
      661 agcagaacgc cgtccggaat cgcttcattt ttcgccactc gcttttggat ctcacccatg
      721 ctcatcgatg cggtcgcgaa gtcgaggata atcgatttgc cattgcgcgc gggaataccg
      781 aaggccattg ggttggtgcc ggtgatcggt ttcatgccgc cgtagagcgt ggtggtcgcc
      841 gagccgacgc tgactgcgcc catgccgatg agtccttgcg tcgttgccat ttccatataa
      901 tcggcgagcc gcccggtgtg tccggcgcga cgcacgcaca cgattcccgc gccgtgctcc
      961 ctagctttct tgatcgcaag ctctaccgcc atacgcgctg cgatctgccc aacgtagtga
     1021 tgaccatcga tcactgccgt gattttgcct tcacgctcga tttgcgggcg cgcgctgaga
     1081 tcgtaagtct tatttctgat aaatcccaaa tactggccga ctcgggtgac gccgtggcct
     1141 gcgtaaccgc ggagttctgc tttcacgagc aattcggcga cgaatccagc atcggctccg
     1201 ggcattccgc cggcggcgag cagtgcggcg cagaaatcgc gcagcggagc aggcttcagt
     1261 tttggcacgg cggttatttc tagcttggcc gaaccggaaa ataacgctgc agccattcgt
     1321 gaacgatgtt cgtcaaacgc ggattttctt cgttgaaggt aaagtgatcg acatcggcga
     1381 gcaaatgcaa ttcggccggt tgtcgtgctc gcttgaacat ctcgatagat tcataggtgg
     1441 gtgtcaccga atcattagcg ctgtggagca ataacagcgg acgtggggaa atatttccca
     1501 cttgatcatc ggcgcgaaaa tcgttcatgc tctgcgcagt ttcggcggga aattccataa
     1561 tcgagccgct gctcatacga ttacgcaatt tttcgggaat cggcacgata tcgtaacgcg
     1621 gaaccatgag cgatttgccg gtacgttcgc ggtgtcgctt gccgtcttcg agcatctggg
     1681 tgaattttgc ccaagcctcc ggggtcgggt gctgaccgcg aaatttgcgc tcgccattgc
     1741 cccaaccgcc acaagaaata actgcggcga cgcggcgatc ggcgccgccc gtaaacaccg
     1801 caaccgcggc gccgaagctg ctgccaacga gcgcaatttt gtcgggagcg acttccggcc
     1861 gagtggtgag atacgagatg gcatttcggg tgtcggctac ttggtcgagg cagatgatac
     1921 gcccgtgctc accctcgctt tcgccgcagc cgcgaaaatc gatgcgcaag gtgacgtatc
     1981 cccattgagc gaattgtttc gcgacaaccg cctggccctg cccgtctttg ttgccgccaa
     2041 aaccatgtaa aacgataaag gccggccggc gttcaccggg tttatagtcc ggcggcgtgt
     2101 cgatcaaacc ggaaattttc aaaccatcgg attgaaatga aatcttctct gccatcgatt
     2161 atctcctgat tttgcgctgc aagtttgttg tgtcaacgct cgtgtactca ggaggctcag
     2221 tttcgtccga cttcccgagt agcgcgaagc acgtatcgag ggatatgcga tcggtcaggt
     2281 caatgtcctc cctcatcccc acggacctaa aaacggttcg ccgagatagg catcggcggc
     2341 gtccaaggat tctggccact cgctgagact gcgcggcctg cgcaattctt tcggccgtgg
     2401 cttaccgtcc cagccgattt gttccatggc acagtagagt tgaatggtgt gaccatccgg
     2461 atcccgcacg gttgcagaat attcaatgcc gggatgtagc tcagcgggaa tcgattgcat
     2521 caccgggatg ccgttttcac gcaaaaactt cacggcatct ttgagctggc gataattcgc
     2581 cagctggatg ccgaacgagg cgcacttgga atgcgggctc aagccgaaag catcgcgcag
     2641 ctcgagagga aaaagagcga gcgagtggtg ctcggtatta cagcggaaaa agaaacagcg
     2701 ttcaccttgc cagctgacct cttcggttaa agtaaaaccc aaagtatctc ggtaaaattc
     2761 caccgcagca gccatgtttt cactgaatag gtagaccgga ccgagccgga cgatcttgaa
     2821 cggacgcggc aacagaatgc catcgacatc atagttggcg ggcagcttct cgagataccg
     2881 gtagccggag aagatatcga cgccattggc tcgggcttct tcgacttctt gaaactcgga
     2941 aatttgcggc agctccggcg gcttgtcgaa gccgcgatcg tacatcgcgc gcggcttgct
     3001 atggccgttc cagccgattt gctcgatgcc gtagtatagt tcgttactct ggccgtcagg
     3061 atcataaaga taggtatgcc aattggatcc gggcatgtcg cggcctgaac gctgctgctt
     3121 gatgtcgcgc tgattgaacc atttgattgc cgcgccgacc tcggcgaggc tgccgacttg
     3181 ccacgtgatc tgattgacgg taatgcccgg ccgctcctgt cgcccaagcg cgtcgcgcac
     3241 gcgcttggga aagagcacca tggcatgatg atcggtacca taccgcgtga aatagccatt
     3301 gggatcgccg aggccggcga cctcggccgg atctttagcg cggcgcgagt aatccattac
     3361 gtcggaaatg cgaaaaccga gcaggtcgac ataaaaccgc acgccgtctt ccatattgga
     3421 aaggttaaag ccgaaatggc cgagacggcg aatcttgaag ggctgcttta gtagaacgcc
     3481 tccgacatcg aactttgctg cgacttcaga catgatccta cctccaaccc ggttcggcac
     3541 ctctatacaa tcttttccgg cgactcacaa tgtgaattca gacagcttgc aacgacacga
     3601 gtgctggttt atcaatatgc cgtctgccga aactactcga tgtccagtta acatactctt
     3661 aactccgaaa atcaaggagg gttgaatgcc gcttcaagaa ctacacgcta atggcaaagt
     3721 tcaatggccg ggcgaagcgc ggatcgcggt ctttttgagt ttcgactttc agggcgggga
     3781 agatgtgaag cctgacaaga acggccacat caatcacgag gaatacaccc agggcgaata
     3841 cggtcccaat acagggatct ggcggattct acggattctt tctagtcacg aagtgagagc
     3901 cacatttctt acctgcggcg ggatcgccga acgttatccc gaagctctag cggccattgc
     3961 cgctcaaggc catgaactcg ccggtcatgg ttatcaccat gaagtggcgc gcgacttgag
     4021 caaagaagaa gagcgcgacg tgatcaaacg aaccacggac atgattctca atcgcgcgca
     4081 gaagcggccc tttggctggc gctcgtgtac gcagagcccg aacagtttgg agctgttatt
     4141 ggaacaaggc tatctgtgga acagcaattc gttcgcgcat gatctgccgt ttgtttggca
     4201 aaacgggaag aaaaaattgg tcgagttgcc gcgtcagccg ttcggcgatg ggcgcacgta
     4261 tggacaccgt gattcaggaa accccaacga tactttggtg gtctggaagg cgatgttcga
     4321 tcaattctat gaggaatcgg ctttggcccc gaccttctgc ccgttccaat ttcatcccta
     4381 tatttccagc cgtccgggcc gcgccaagac gttatcggaa atcattgcgt acatgaaatc
     4441 gcataaaggc gtgtggttcg
//
LOCUS       DASULZ010000535         3469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1383163, whole genome shotgun sequence.
ACCESSION   DASULZ010000535 DASULZ010000000
VERSION     DASULZ010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383163"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            325..840
                     /locus_tag="VFY96_10970"
     CDS             325..840
                     /locus_tag="VFY96_10970"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEX5607029.1"
                     /translation="MSKIRTFVLATSVFAISGLILANIPMAASSDTNAPAKPGFARGR
                     MGPGFFGAPLITIALNHKSELNLTTDQVTNLENIRSHYQSQVTPVQQQLSSMEKEIAS
                     LMQQTPANLVQIKSKLQDAEKYRTELRYLRIEALENGRSVLTDQQKEQLKTLVRSRHG
                     HSRRAPQGQPS"
     gene            964..1653
                     /locus_tag="VFY96_10975"
     CDS             964..1653
                     /locus_tag="VFY96_10975"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX5607030.1"
                     /translation="MTVSALIFDLDGLLADTERLHCQAYQMTFLEQGITLQELDYCEH
                     WVRFGRGITDWVMLNKLTVDPYSLRLRKSEHYSALLSSSLRPMDGALELLDALHGQIR
                     FALASSSYRDAINGVLMGLNIAHFFDVVVSGLDVPAVKPAPDIFLKAAHDLGVRPEEC
                     VVLEDAEKGVLAAHSAGMRCIAVPNEYTRHHDFSKATRICSSLKEITPKLLQSLEPHA
                     TPTNSRDHLSS"
     gene            1634..2368
                     /locus_tag="VFY96_10980"
     CDS             1634..2368
                     /locus_tag="VFY96_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607031.1"
                     /translation="MIIYLLNRYYWLGLLVALLAPLWVRAASGNADQFAETKDALIWT
                     GVLLGSRLLMTKYPDFAVRGLWRAPTRKQLKMYLRLVLGLIIVDYGSKAIFFRWDRPL
                     QVEVFKNFGLHSVYHVHPLEVFHLILYLYFLYVFLVGALFFRFSDKWLDRLWLLSSTS
                     ALGGATALFGERLLFGGVQNSFYFAGPLMWLCPPCASPHFSSYAWTPADFFVHAAFAP
                     VLILIVSYFIPMRSSVLALDSQQPST"
     gene            2439..3443
                     /locus_tag="VFY96_10985"
     CDS             2439..3443
                     /locus_tag="VFY96_10985"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5607032.1"
                     /translation="MASAELIVDVHHHYMPAMLFDRLAKQAGDKRIVTNEISLTLHPS
                     RKDLDTHIKCMDEAGIAVCILTDQVQVMGHEVARALNDGIAEVEKKYPSRFRGCIHLP
                     VHEPAAAQRELERGIHELGLRAVALLACHLDVQLDNPIMNPLFEVIQKNALPIVIHPQ
                     SKPTGSDTLYNLDRCVFRPLETTQAIVRVMNSVLPRYPDLRFIMPHLGGGTSALKGRM
                     MAFFETEDADVPSDMRGYLKTQSEQKKFGLTERFEKLFQRLYFDTAGTGAWLPAMAAA
                     FNITTPDRIMFGSDYPLECKTAANLTESINMVRQAPCSSDEKTAILGKNAAALFNLKP
                     "
BASE COUNT          775 a          956 c          891 g          847 t
ORIGIN      
        1 cgagaagcgg atcgaaatcg cacggacgat cgcccaacga aggcttgcgg cagacgagat
       61 gcagcaaccc caatcatcgc gttactgatc tcacgttttg gccgcgttcg acttgctctc
      121 gaaacgacta tgagggccaa gttgacccat gattcatgag gctacggtcc gcgaatagcg
      181 ttctgcgaag cacctcccag ttcaacagct gaccttctaa ttaggctagg cacagcacac
      241 cagagaccgg caaattccga gcacgtaaac ttttacgggg ccacttcgtc taattggcag
      301 tgtcgagtac cttgaggagg gtttatgagc aaaattcgta cgttcgttct ggcaactagc
      361 gtgttcgcaa tatccggcct gatactggct aatataccga tggctgcctc gtcagatacc
      421 aacgcgccgg ctaaaccagg gttcgctcga gggcgcatgg gcccgggctt ttttggcgct
      481 ccgctgatca cgattgcgct aaatcataaa agcgaattga atttgactac ggaccaagtc
      541 acgaatttgg aaaacattcg tagtcattat caaagccagg tgaccccggt tcagcagcag
      601 ttgtccagta tggaaaaaga aatcgcaagc ctgatgcagc agacgccggc taatttggtg
      661 cagatcaaga gcaagcttca ggacgccgaa aagtatcgaa ccgaactccg ctatctccgt
      721 atcgaagcgc ttgaaaatgg ccgctcagtt ctcacggatc agcaaaagga acaattgaaa
      781 actctggttc ggtcgcgcca cgggcattcc cgccgcgcac ctcagggtca gccctcataa
      841 ttcctacgca acaaattgaa aaaaggcctg gctcatgagc cgggcctttt ttttgaatcc
      901 gtcggctggg ttggctgcga aaggcaaaat ccagtaaaat tggtgtgtaa ggagcgccga
      961 aaagtgactg tcagcgcgct gatttttgac ctggatggat tgctcgcaga taccgagcgg
     1021 ttgcattgcc aggcttatca aatgacattt ctggagcaag gcatcacgct tcaggaactc
     1081 gattattgcg agcactgggt tcgcttcggc cgaggcatta cagattgggt catgctcaat
     1141 aagttgaccg tcgatccata ctccctgcgg ttacgcaaat ccgagcacta ttccgccttg
     1201 ctcagctcaa gtctacgtcc aatggacggc gcgctcgagt tgctcgacgc gttgcatggc
     1261 cagatcaggt ttgcgctcgc ctcgtcttcc taccgcgacg cgatcaacgg cgtattaatg
     1321 ggccttaaca ttgcgcattt ttttgatgtc gttgtcagcg gtctggatgt gcccgccgtt
     1381 aaaccggccc ctgatatttt tcttaaagcg gcccatgacc ttggcgtcag gccagaggaa
     1441 tgtgtcgtat tggaagacgc ggaaaaaggg gttttggccg cacactcggc cgggatgcgc
     1501 tgcattgcag tgccgaacga gtatacccgc caccacgatt tctccaaggc aaccaggatc
     1561 tgctcatcat tgaaggagat cactccgaaa cttctgcaat cactggagcc gcacgcgacc
     1621 ccgacgaaca gccgtgatca tttatcttct taaccggtat tattggcttg gcttgctggt
     1681 ggctctcctg gcaccgcttt gggtacgcgc tgcgtccggc aacgctgatc agttcgcaga
     1741 aacaaaagac gcgctaatct ggacgggggt cttgctcggc tctcggctgc tgatgacaaa
     1801 gtatccagat tttgcggtgc gaggcctatg gcgtgcgcca acaaggaaac aactaaaaat
     1861 gtatttgcgc ctcgttctcg gtctcatcat cgtcgattac ggctccaagg cgattttttt
     1921 tcggtgggat cggccgcttc aggtcgaagt gttcaaaaat tttggtctgc actcggttta
     1981 tcatgtgcat ccgctcgagg tctttcacct gatcctttat ctatattttc tttatgtttt
     2041 tcttgtcggc gcattgttct ttcggttctc agataagtgg ctggatcgcc tctggctcct
     2101 atcctctacg tccgccctag gcggcgccac agcgctgttc ggtgaacggc ttctgttcgg
     2161 tggggtacaa aattctttct attttgcggg accgctgatg tggctctgtc cgccctgtgc
     2221 gtcgccgcat ttttccagtt acgcatggac gccggcggat tttttcgtgc acgccgcttt
     2281 cgctcccgtg ctcattctaa ttgtttccta tttcattcct atgcgttcct cggtgctcgc
     2341 gttggattcg cagcagccct cgacgtaacg cttgcagtgt tgagtaccgt gacatagttt
     2401 ggtgagcatc aattcacgtc aaggagtccc acacaaatat ggcttcagca gagcttatcg
     2461 tcgatgtgca ccatcattat atgccggcga tgttgttcga tcgcctcgca aaacaagcag
     2521 gcgacaagcg catcgtcacc aacgaaatca gcttaaccct ccatccgtcc cgaaaagact
     2581 tggatactca catcaagtgc atggatgagg cgggtatcgc ggtttgcatt ctgaccgacc
     2641 aggttcaagt gatgggacac gaagttgctc gagcgctcaa tgacggcatt gccgaagttg
     2701 aaaaaaaata tccgagccgc ttccgcggtt gcattcacct gccggtccac gagcccgcag
     2761 cggcgcaacg tgagctcgag cgcggcatcc atgagctggg gctgcgcgcc gttgcgcttc
     2821 tcgcctgtca tctcgatgtg cagctcgata atcccatcat gaatccgctt ttcgaagtca
     2881 ttcaaaaaaa cgctctcccc atagtgatcc acccgcagtc caagccgacc ggctccgata
     2941 cgttgtacaa tctggaccgc tgtgtgttcc gtccgctcga aacgacccag gcgatcgtgc
     3001 gcgtcatgaa cagcgtctta ccgcgctatc cggacctccg cttcatcatg ccgcacctcg
     3061 gcggcggcac ctcggcgctc aaaggccgta tgatggcgtt tttcgaaacc gaagatgccg
     3121 acgtgccaag cgatatgcgc ggatatttga aaactcagtc ggaacagaag aaattcggcc
     3181 tgactgagcg ttttgaaaaa ctttttcagc gcctctactt cgacaccgca gggaccggcg
     3241 cctggttgcc cgccatggcg gcggccttca acatcaccac gcccgatcga attatgttcg
     3301 gtagcgatta tccgctggaa tgcaaaaccg cagcgaacct aaccgagtcc atcaatatgg
     3361 tgcgccaggc tccatgcagt agtgatgaaa aaacagcgat tcttggaaaa aacgccgcgg
     3421 cgctttttaa tttaaaacca tgaaattgat ttctttgctc tttctgctc
//
LOCUS       DASULZ010000536         2495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_265808, whole genome shotgun sequence.
ACCESSION   DASULZ010000536 DASULZ010000000
VERSION     DASULZ010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265808"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1381)
                     /locus_tag="VFY96_10990"
     CDS             complement(<1..1381)
                     /locus_tag="VFY96_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(806..808),aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5607033.1"
                     /translation="MEVSRRDFLRFSVAAGTGTALSGLVGSGVNLSPTVARAQELRIK
                     GAKTTPSLCPYCSVGCATLVNTIDGKIVNIEGDPRSPHNEGTLCPKGAAIFQLHVNPN
                     RPTQVLHRAAGAADWEVWDIDRAMTRITELVKKTRDDTFIEKLPDGKLVNMTPAIFAL
                     GGATLEIEFNHVCQKLMRGLGVVAIENQARIUHSSSVPGLGTRFGRGAATLYPRSMYD
                     SDCIIVMGSNMAENHPVAFRWPMKAKVERGAKIIHVDPRFTRTSAVADIYAPVRAGSD
                     IAFLGGVINYVINSEKWNTDPFFKTFVVNYTNATTIINEEFKDTEDLDGVFSGLLEAK
                     GGVAEWPFNGFINQYDGATWQYARTKIGDQGRTASTAQSGETAGTKSGQQPQAAPGAS
                     AGASQGPPFDNLVKSLVKPAPVRDETLQDPRCVLQIVKTHFARYTPEMVEKTTGCPKE
                     TFLKVAETIL"
     gene            1810..>2495
                     /locus_tag="VFY96_10995"
     CDS             1810..>2495
                     /locus_tag="VFY96_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX5607034.1"
                     /translation="MAKPRVRKGTPSVQLSWEEFSRRVRERFYDPSFSAVSREIDAII
                     DIAWKNYTEYHKSPRKQPAGAGFSDPKFELPIEWLQTRKAIRQAQNRQKNPRSNSRVL
                     LINGSTRSDQTCPGEMSKTYRLVKMAEEIITVEKGFEVDLLDLSRLASEYGRIIYPCK
                     ACVSTAMPLCHWPCSCYPNHAMGQVNDWMAEIYPRWVAAHGIMVLCPVHWYQAPSSLK
                     LMIDRLVCADG"
BASE COUNT          524 a          669 c          728 g          574 t
ORIGIN      
        1 ccagaatggt ctcagcaact ttcagaaacg tttccttggg gcaaccggta gtcttctcca
       61 ccatctccgg cgtgtagcgt gcgaaatgcg tcttgacgat ctgcaaaaca cagcgggggt
      121 cctggagcgt ctcgtcacga accggcgcgg gcttaactag cgacttcacc agattgtcga
      181 acggaggtcc ctggctggcg cctgcgctcg cccccggcgc ggcttgcggc tgctgacctg
      241 atttggttcc agccgtttct ccggactggg cggtactggc cgtgcgtccc tggtcgccaa
      301 tcttggtccg cgcgtactgc cacgtcgcgc cgtcgtactg gttgataaat ccattgaatg
      361 gccattcggc cactccgcct ttggcttcca ataatcccga gaacaccccg tccagatcct
      421 cggtatcttt gaactcctcg ttgatgatcg tggtggcgtt ggtgtagttg acgacgaagg
      481 ttttaaagaa cgggtcggta ttccacttct cgctgttgat gacgtagttg atgacgccgc
      541 ccaaaaaggc gatgtcggag ccggcgcgaa ccggagcgta gatatcggcg acggcgctgg
      601 tccgggtaaa gcgcggatcg acgtgaatga ttttggcccc gcgctccacc ttggccttca
      661 tgggccagcg aaaagcaacc ggatggttct ccgccatatt ggagcccatc acgatgatgc
      721 agtcggaatc gtacatgctg cgggggtaga gagtcgccgc gccgcggccg aagcgtgtcc
      781 ccagaccggg gacgctagag ctgtgtcata tccgcgcttg gttctcgatc gccacgacgc
      841 cgagaccgcg catgagcttc tggcaaacgt gattgaactc gatctcgaga gttgcgccgc
      901 cgagagcgaa gatcgccggt gtcatattga cgagcttacc gtcgggaagc ttttcgatga
      961 acgtgtcgtc gcgggtcttt tttacgagct cggtgatccg ggtcatcgcg cggtcgatgt
     1021 cccatacttc ccagtccgct gctcccgccg cacgatgcaa aacttgagtg ggacgattcg
     1081 gattgacgtg gagctgaaag atcgctgctc ccttcgggca gagagtgcct tcgttgtggg
     1141 gacttctcgg gtcgccttca atgttgacga tcttgccgtc gatcgtgttc accaaggtgg
     1201 cgcagccgac ggaacagtag gggcagagac tcggagtggt tttcgctcct ttgatgcgga
     1261 gctcctgggc gcgagcgact gtcgggctca ggtttacgcc ggaacccacc aggccgctga
     1321 gcgcggtgcc tgtgcctgct gccactgaga aacgaagaaa atcccgtcgt gatacttcca
     1381 tgttataact ccttcgtcag ccgacactcc cctaaaagaa cgggagaaaa catgctttcg
     1441 ctctgtgcag tgaaaaaagt acgtttcact cggcacaggc tcacgagcgg tggtgatatt
     1501 agcgccgaag gaattcttgt caagaggggg tattcgactg tcatcgatgg cggacaaacg
     1561 aattttgttt catcggagca gtagctttct caattccaga atggccattc gactttcacc
     1621 tggcgatcgt cgttgctttg ggaaatttga tctgcgaagg gcaaccaaat gtgcggcatg
     1681 gcgcgcgtgc gacatttaat aggttgggct gcaagcgttg ctggaagcta ggggaggtca
     1741 tatgcgcctg caaagcttat tatggtgtaa tacttcggcc tcatcctttg cgcatcgtaa
     1801 ggaggaataa tggcaaagcc gagagtacga aagggaacac cgagcgttca gctgtcatgg
     1861 gaagaatttt cacggcgtgt gcgcgaacgg ttctatgatc caagtttttc tgcggtgagt
     1921 cgtgagatcg atgccatcat cgatatcgcg tggaaaaact atacggaata ccacaagagc
     1981 ccgcgcaagc agcccgcagg cgcaggattt agcgatccga agtttgaact cccgatcgaa
     2041 tggctccaaa cgcggaaagc tattcgtcag gcgcaaaatc ggcaaaagaa tcctcgatcc
     2101 aattcgcgcg ttctccttat caacggctca acgcgcagcg accagacctg tccaggtgag
     2161 atgtcaaaaa cttatcgcct ggtcaaaatg gccgaagaaa taattacggt cgaaaaaggg
     2221 ttcgaggtcg atctgctcga tctcagccgg cttgcttccg aatacggccg cattatctac
     2281 ccgtgtaaag cttgtgtttc gaccgccatg ccgctctgcc actggccgtg ctcatgttat
     2341 ccgaatcatg cgatgggaca ggtgaacgat tggatggctg aaatctatcc gcgttgggtg
     2401 gcggctcacg ggatcatggt tctctgtccg gttcattggt atcaagcacc cagctcacta
     2461 aagcttatga tcgatcgcct ggtgtgtgcc gacgg
//
LOCUS       DASULZ010000537         3555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1280914, whole genome shotgun sequence.
ACCESSION   DASULZ010000537 DASULZ010000000
VERSION     DASULZ010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280914"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2932
                     /locus_tag="VFY96_11000"
     CDS             <1..2932
                     /locus_tag="VFY96_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002730090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEX5607035.1"
                     /translation="TSNNRGPAGLWRYGISGDLPIVLIRIDEAEDQNIVRQLLRAHEY
                     WRMKQLAVDLVILNEQPPSYAQNLQDALEAQVRASQSHLRQEGQESFGGVFILRGDLI
                     SAEDRTLLQTAARAVLLSRRGTLAEQVERQQRLQTPVSPLPRRLPPPKSTDALPVRQE
                     LEFFNGLGGFTADGREYVTILGEGQWTPAPWINVIANACFGFQISESGSGYTWSGNSR
                     ENQLTPWSNDPVSDPPGETIYVRDEDSGELWGPTVLPIREEAWPYTARHGQGYSRFEH
                     TSHGVSLDLMQFVPLEDPIKISRLTIENLSGRSRRLSVTAYVEWVLGVSRSTSAPFVV
                     SEIDTETGAMLARNAWNSEFAGRVAFADLSGRQTTWTGDRTEFLGRNGTPDHPASLER
                     GERLSGKVGAGLDPCAALQTIVELRAGGRAEIVFFLGQAASAEEARILIALYRGADLD
                     ATLRTISKRWDDVLGTVQVTTPDRAMNFMLNRWLLYQTLACRIWARSAFYQAGGAYGF
                     RDQLQDVMALMVAQRGIARAQLLRAAARQFVEGDVQHWWHPPSGRGVRTRVSDDLLWL
                     PYVTIQYLELTDDVALLDELVPFLDGPTLAPGKEDSYFEPRVSAQTGTFFEHCARALD
                     RSLAVGSHGLPLIGTGDWNDGMNRVGSAGKGESVWLGWFLHTTLWEFARLADMRGEHQ
                     RAETWRLHVSALKAALEREAWDGEWYRRAYFDDGTPLGSAGNAECRIDSIAQSWGVIS
                     GAAERGRGARAMAAVEKYLVRSRDGLVLLFTPPFDQATPDPGYIKGYLPGIRENGGQY
                     THAAIWSVLAFAALGDGDKAGELFSLLNPINHASTRAGVYRYKVEPYVVAADVYAEPP
                     HVGRGGWTWYTGSAGWMYRAGIEWILGFHLRGTRLYFDPSIPRAWRRFEISFLYHSSR
                     YQIVVENPQGVTRGLLSVELDGALLATNNMYIQLADDGATHHVRVKLGSEILAVGRQA
                     TTPV"
     gene            3261..3467
                     /locus_tag="VFY96_11005"
     CDS             3261..3467
                     /locus_tag="VFY96_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607036.1"
                     /translation="MAEEVLLDVKTADAANLYKFFYLNQQKKILGSIAVILFLTLLWL
                     SRVDMETKWKNSADVERLRFAAMR"
BASE COUNT          718 a         1035 c         1061 g          741 t
ORIGIN      
        1 cacaagtaat aaccgggggc ctgcggggct atggcgatac gggatttccg gcgatctgcc
       61 catcgtcctc atccgcatcg atgaggccga agaccagaat attgtacgtc agttactccg
      121 cgcccacgaa tactggcgga tgaagcagtt ggcggtggat ctcgtgatct tgaacgaaca
      181 gcctccctct tacgcgcaaa acctccagga tgctttggag gcccaggtcc gcgccagcca
      241 gtcgcacctg cggcaggagg ggcaggagtc gttcggcggc gtattcattc tcagaggaga
      301 tctgatttcg gccgaggatc gcacactgct ccagactgcc gcccgggccg tactcttgag
      361 ccgccgcggc accttggctg agcaagtgga acgtcagcaa cgattgcaaa ctcctgtgtc
      421 gccactgccg cggcggctgc cgccgcctaa gtcaaccgat gccttgcctg tgcgccagga
      481 actggagttc ttcaatggcc tgggtggatt cactgctgac ggccgtgaat acgtgacaat
      541 tctcggcgag gggcagtgga cgccggcgcc ttggatcaac gtgatcgcga acgcctgctt
      601 cggctttcag atctccgaat cgggctcggg ttatacgtgg tcgggcaata gccgtgagaa
      661 ccagctcacc ccgtggtcga atgacccggt tagcgatccg ccgggagaga caatatacgt
      721 ccgcgatgaa gatagtggcg agctgtgggg tccgacggtg ctgccgatcc gcgaggaggc
      781 ttggccctat acggctcgac acggccaggg ctacagccgc tttgagcata catcacacgg
      841 agtgtcgctc gacctcatgc agttcgtgcc actcgaggac ccaatcaaga tttctcgcct
      901 caccatcgag aatctttccg gccgatccag acgtctctcc gtaaccgcct atgtcgagtg
      961 ggtgctcggc gtttcgcgga gcacctccgc gcctttcgtc gtcagcgaaa tcgacacgga
     1021 aacgggggcc atgctcgcgc gcaacgcctg gaacagcgag ttcgctggcc gcgtggcttt
     1081 tgccgatcta agcggccgcc aaaccacgtg gacgggtgat cggacggagt ttctcggtcg
     1141 caacgggaca cccgaccatc cggcatctct cgaacgaggg gaacgtctct cgggcaaagt
     1201 tggcgccggc ctcgatccat gcgctgccct gcagacaatt gtagagctgc gtgccggtgg
     1261 gcgcgccgaa atcgtctttt tccttggcca ggcggcgagc gcggaagaag cgcgaatact
     1321 gatcgcgctt tatcgcggcg ccgacctcga tgcgaccctg cgcacaatca gcaagcgctg
     1381 ggatgatgtt cttggcacgg tacaggtgac aactcctgat cgggccatga acttcatgct
     1441 caatcgctgg ctgctgtatc agacgctcgc ttgtcgtatc tgggcgcgct cggcgtttta
     1501 tcaagctggc ggcgcctatg gctttcgcga tcagctccaa gacgtgatgg cgctgatggt
     1561 cgcgcaacga ggcatcgctc gggcgcagtt actgcgggcg gccgcgcggc aattcgtcga
     1621 gggagatgtg cagcattggt ggcatcctcc gtcgggtcgc ggcgtgcgca cccgtgtctc
     1681 cgacgatctc ctatggctgc cgtacgtgac tatccagtat cttgagctca cagacgatgt
     1741 cgctcttctc gacgagcttg tccctttcct cgacggtccc actctcgccc cggggaaaga
     1801 agattcgtat ttcgagccgc gcgtgtccgc gcaaaccggc accttcttcg agcattgcgc
     1861 ccgcgctctc gatcgcagtc tggccgttgg cagccacggt ctgccgctga tcggtacggg
     1921 agactggaat gacggaatga accgggtggg atcggcagga aaaggcgaga gcgtctggct
     1981 cgggtggttt cttcacacga ccctctggga atttgcccgg cttgcggata tgcgcggcga
     2041 acaccagcgc gccgagacgt ggcgactgca tgtcagcgcc cttaaagcag cgctggaacg
     2101 agaagcttgg gatggcgaat ggtatcgacg cgcttatttc gacgacggga caccgctcgg
     2161 atctgccggg aacgccgaat gccgcatcga ttcaatcgcc caatcctggg gagtcatttc
     2221 cggtgccgct gagcggggtc gtggagcgcg tgccatggca gcagtcgaga agtatctcgt
     2281 tcgaagccgc gacggtctgg tgctcctctt caccccgcct ttcgaccagg caacccccga
     2341 ccctgggtac atcaaaggat acctgcccgg catccgcgaa aacggcggtc aatacactca
     2401 tgcggcgatc tggtctgtgc tcgccttcgc cgcgctcggc gatggcgata aagccggcga
     2461 acttttctcg ctgttaaacc cgatcaatca cgcgagcacc cgcgctggtg tttatcggta
     2521 caaggtcgag ccctatgtcg tcgcggcgga tgtttatgcc gaaccgccgc acgtcgggcg
     2581 cggtggttgg acgtggtaca ccgggtcagc gggatggatg tatcgcgcgg gcatcgagtg
     2641 gatcttgggt ttccatctgc ggggcacgag gctttacttc gacccgtcca tcccgcgcgc
     2701 gtggcggcgc tttgaaatca gcttcctcta tcactcatcg cgctatcaaa tcgttgtcga
     2761 gaaccctcag ggagttacac gtggattgtt gtctgttgaa ctcgatgggg cactgctcgc
     2821 aactaacaat atgtatatcc agctcgccga cgacggtgcg acgcatcacg tgcgcgtcaa
     2881 gctggggtcg gaaatcctcg ccgtcggccg ccaagctacg acaccggtct gacacgagag
     2941 ctggcagctt gacgctcaag cccagcggaa aatcgttctg gtccgaagag gcgaaacata
     3001 ttcggtcgag gccgatccgc agacgctgcg gatgccccct tcgctagcac ccacaaaaag
     3061 gcgcgcattc gcacgccata aatttacttc agaaccgccc aattgtagac agcaatcttc
     3121 cagcattagc gtatcctgat cggctcagat ccgataacgc cagacaactc tgaaataacg
     3181 aagatcagcg ttcactttca ggaggtctcg ttcgaaactt tcgacgtgaa aaatagggac
     3241 ggttggctga gcgcaatgta atggctgaag aagtgctatt ggatgtcaaa acagctgacg
     3301 cagctaatct ctacaagttt ttttatctca atcagcaaaa aaagatctta ggatcaatcg
     3361 cggtgatcct ttttctcact ctcttatggt tgagtcgagt tgatatggaa acaaaatgga
     3421 aaaactcggc agatgttgaa cgtttgcgat tcgccgcgat gagataaatg gaggcgtaaa
     3481 atagagccgc gatcaaggaa gcggcactgc gcacgttaat tatgtcatgt cgctgatcgg
     3541 agtcagtcca tattc
//
LOCUS       DASULZ010000538         3215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1076919, whole genome shotgun sequence.
ACCESSION   DASULZ010000538 DASULZ010000000
VERSION     DASULZ010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076919"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..678
                     /gene="mraZ"
                     /locus_tag="VFY96_11010"
     CDS             226..678
                     /gene="mraZ"
                     /locus_tag="VFY96_11010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX5607037.1"
                     /translation="MFRGSFEHTIDNKGRLSIPSKFRDVLVGKGDERIVMTNFVIDGK
                     RCLDVYPVDAWLRFEDEIRKKPKFDPRMVRFQNYYLGSACECSPDDHGRILIPPTLRK
                     YADLKRDVVLVSALEKFRVWDREAWKQIFVEAEDKLMQDPDFLGDLGL"
     gene            712..1074
                     /locus_tag="VFY96_11015"
     CDS             712..1074
                     /locus_tag="VFY96_11015"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5607038.1"
                     /translation="MLARKPIQDISVLDVTGDIDFREMIRIKEAIGSLIEKERTKVVL
                     NLKSVDHINYLSIGVLLERLKLLRNLNGDLKLAGMSPFLRDIFKVVGMDRFFEDYTSL
                     EDAIESFDDDWEGDGTSH"
     gene            1076..2002
                     /gene="rsmH"
                     /locus_tag="VFY96_11020"
     CDS             1076..2002
                     /gene="rsmH"
                     /locus_tag="VFY96_11020"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005987936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX5607039.1"
                     /translation="MYAHVSVLVREVIELLAPASSKRYLDGTLGGGGHAKEILMTSTP
                     DGQLLGLDRDDEAIRAARERLRRFDWRFISRQASFADALGILREIGWHQVDGVVLDLG
                     ISSHHIDASERGFSFRSSARLDMRMDRRQDLDAERIVNSATQAELEQIFRDYGEEPQA
                     RRIAQVIVRERQTRSLETTADLVQVIEKIKGRGRRGHHPATQVFQALRMAVNQELDHL
                     GRFLENGYETLRPQGRMAIISFHSLEDRMVKTKFRQWSRDCLCPPRTLRCQCGWSRKV
                     KLLTKKPVVPSAAEIHANPRARSAKLRAVERI"
     gene            2040..2354
                     /locus_tag="VFY96_11025"
     CDS             2040..2354
                     /locus_tag="VFY96_11025"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEX5607040.1"
                     /translation="MPAVKRERPLRREHKLNRRQFVCVALLGLLLVGVVLVHVWLRLQ
                     VVKTGYVLAIATKLQTRLEQENRELQIDLATLTSPDRLGSLARRRLGLAPPEKGQVII
                     LP"
     gene            2351..>3215
                     /locus_tag="VFY96_11030"
     CDS             2351..>3215
                     /locus_tag="VFY96_11030"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX5607041.1"
                     /translation="MRRSTQRNVRRIVAAKILFAVLFLSIGGRAIQLQLWEGDKLMRL
                     GQRQHLKEWIVLPKRGAVFDRTGEPLALSMESQSVYARPHRIQDPTPVARSLAPILNM
                     PLAEIKQKITSEKPFVWVKRQISSAEAEKIQALNVDGIGMFYEPHRQYPQGQLAGQLI
                     GFVGRDSEGLEGLELKYNDYIRGETGSSIAERDALGRRVLVQGVEGLHIPPGSDIHLT
                     VDTSIQYMAEKELEASVMKYRAKAGIAIVVEPFTGEVLALANYPSFNPNNYAKQSPEQ
                     RRNRAVTDSFEP"
BASE COUNT          748 a          806 c          935 g          726 t
ORIGIN      
        1 ttgttccccg tacattttgc agcctgctct tcgggttttt gtgtctctga acgacgctca
       61 gttttaatcg gttcgagagc gaattcagcc gaacaaaaaa acttccgaaa acccctaaaa
      121 ctcaggtctt ttttattgac aggtggaacc gaacgaacta taagtggcgc ttagtgggaa
      181 aaggtgggat aaaaacctat tttacccatt gattaaatat aaactatgtt tcgcggcagc
      241 tttgagcaca ccatagacaa taagggacgg ctgagcattc cgtcgaagtt tcgcgacgtt
      301 ctggtaggta agggcgatga gcgtatcgtg atgaccaatt tcgtgatcga cggcaagcgc
      361 tgcctcgatg tgtatcctgt cgatgcatgg ctacgcttcg aggatgaaat ccgcaagaag
      421 ccaaaattcg atccacgcat ggtgcgattt caaaactact atctcgggtc tgcctgcgaa
      481 tgtagccctg acgaccacgg gcgaattctt attccaccga cgctcagaaa atacgctgat
      541 ctcaagcgcg acgtcgttct ggtttcggcc ttggaaaagt ttcgggtttg ggaccgcgag
      601 gcgtggaagc aaattttcgt cgaggctgaa gacaaattaa tgcaggatcc agatttcctc
      661 ggtgatttgg gactctaacg tgattcccga gcggcgaaag gagaattacg aatgctagcg
      721 cgaaaaccga ttcaggatat ctcagtgctg gacgtcactg gcgatatcga ttttcgtgag
      781 atgatccgca tcaaagaggc gatcggttcg ttgatcgaaa aagagcggac caaagtcgtt
      841 ctcaatctca agtcggtgga tcacatcaat tatctgagca tcggtgtgtt actggagcgg
      901 ctgaagctac ttagaaattt gaacggcgac ctcaagctcg cgggaatgag tccattcctg
      961 cgcgacatct ttaaagtcgt gggtatggac cgcttcttcg aggattatac gtctctggaa
     1021 gatgccatcg aaagtttcga tgatgattgg gagggcgacg gtacgagcca ctgaggtgta
     1081 cgcgcacgtt tctgtcttgg tccgggaagt gatcgagttg ctcgctcccg catcgagcaa
     1141 acgatatctg gatggaacat tgggaggagg cggtcacgcc aaggaaattt taatgacgag
     1201 cactcccgac gggcaactct tgggactgga tcgggacgat gaagcgatcc gtgctgcacg
     1261 ggaacggttg cgccggtttg actggcgctt catcagtcga caagcaagct tcgccgacgc
     1321 tttgggtatt ctgcgggaaa tcggctggca tcaagtggat ggagttgtgt tggatttagg
     1381 catttcgtcc catcatattg atgcctcgga acgcgggttc agttttcgct cgtcggcgcg
     1441 gctggatatg cggatggatc gccgccagga tttagatgct gagcggatcg tcaactcagc
     1501 gacgcaagcc gagttggagc agatctttcg ggattacggc gaggagccgc aggcacggcg
     1561 catcgcccaa gtcatcgtca gagaacgaca gactcgatca ttggaaacta ccgccgatct
     1621 cgttcaggtc attgagaaga taaagggccg cggccgacgc ggacatcatc cggcgaccca
     1681 agtgtttcaa gcgctgcgaa tggctgtgaa tcaagagctg gatcatctcg gccgcttcct
     1741 ggaaaacgga tacgaaacgc ttcggccgca gggacgaatg gcgataatct cgtttcattc
     1801 gctggaagac cgcatggtga aaacgaaatt ccgtcagtgg agccgcgact gtctttgccc
     1861 gccgaggacc ttgcggtgcc aatgcggttg gagtcgaaaa gtgaagctgc tgacgaaaaa
     1921 accggtggtg ccgtcggctg ccgagatcca tgcgaatccg cgcgcgcgtt cggcaaaatt
     1981 gcgcgccgtg gaaaggatct aaggacgtgg ctcgggctgc cagtgtgccg gcgcgccgaa
     2041 tgccggctgt caagcgagag cgtccactgc gccgtgagca caagctcaat cggcgccagt
     2101 ttgtctgtgt cgcgctgctg ggactgcttc tggtcggtgt ggttctagtg catgtgtggt
     2161 tacgcctgca ggtcgtaaag accggatacg tgctcgcgat cgcaaccaaa ttacagacgc
     2221 gtttggagca agaaaaccgc gaactacaga tcgacttggc cactttgact tcacctgatc
     2281 gtctcgggtc gctggcacgc cggcgcttag ggcttgcgcc gcccgagaaa ggtcaggtca
     2341 ttattttgcc atgagaagat cgacgcaacg caacgttcgc cgaatcgttg cagccaagat
     2401 tttgttcgcc gtcctatttt tgtcaattgg cggccgcgcg attcaactcc agctctggga
     2461 aggtgacaag ctcatgcggc ttgggcagcg ccagcatcta aaggaatgga tcgtgctgcc
     2521 caagcgtgga gcagttttcg atcgtaccgg cgagccgctg gcattgagta tggaatcgca
     2581 gtcggtatat gcccggccgc atcggattca agatcccact ccagttgccc gcagtttggc
     2641 gccaattctt aatatgccgc ttgccgagat taagcaaaag atcacttccg agaagccatt
     2701 cgtctgggtg aagcgccaaa tatcatcagc ggaagcggaa aagattcaag ccctcaatgt
     2761 ggacgggatc ggcatgtttt acgagcctca ccgtcagtac ccgcagggtc agttggccgg
     2821 tcagctgatc ggtttcgtcg ggcgcgattc cgagggactc gaaggccttg aactcaaata
     2881 caacgattat attcgcggcg agaccggctc ctccatcgcc gagcgcgatg cgctcgggcg
     2941 gcgtgtgctc gtccaaggag tcgaaggcct gcacattcca ccggggagcg atattcacct
     3001 tacagttgat acttcgatac agtacatggc ggaaaaagag ctggaagcgt cggtgatgaa
     3061 atatcgcgcc aaggccggta tcgccattgt cgtggagcca tttaccggcg aagtgctcgc
     3121 tttggcaaat tatccatcct tcaatccgaa caactacgcc aaacagtcgc ccgaacagcg
     3181 acgtaaccgc gcggtgacgg atagtttcga accgg
//
LOCUS       DASULZ010000539         2741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1483872, whole genome shotgun sequence.
ACCESSION   DASULZ010000539 DASULZ010000000
VERSION     DASULZ010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483872"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..540)
                     /gene="gcvH"
                     /locus_tag="VFY96_11035"
     CDS             complement(88..540)
                     /gene="gcvH"
                     /locus_tag="VFY96_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX5607042.1"
                     /translation="MEFPDGLKYSKEHEWVLVEGKTATIGITEYAQEELGDIVYVELP
                     EIGEKIVKDDPFGAVESVKAVSDVYAPVSGSVLEVNDVLPDNPETINDDPYGDGWMIR
                     VDLSDPDDLKDLMDAEEYAEYVAQQKEEEEEEDDEEDSDEEEEEDEEK"
     gene            complement(573..1667)
                     /gene="gcvT"
                     /locus_tag="VFY96_11040"
     CDS             complement(573..1667)
                     /gene="gcvT"
                     /locus_tag="VFY96_11040"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX5607043.1"
                     /translation="MAPGLKRTPLYAAHRRAAAKMVEFAGWEMPVQYQGVIQEHLAVR
                     RHAGLFDVSHMGEIELRGAGALEFSQRITANDVSRMKVGQAQYNLLLNERGGIVDDVI
                     FYRLAADTFLICVNAANTDKDFIWIQNHAAGAAVTVEDVSAHYCQLALQGPRAQSILA
                     PLTSFDLAGLNSFHFCFADVAAIRCLVARTGYTGEDGFELYCEATAGARLWDALLSAG
                     ESDKLIPAGLGARDTLRLEKAYPLYGHELDDHTTPLEAGLEWVTKFSKPKFSGREALV
                     EQKNRGLGRKLVGLELLGPGIARANYELQKQGRAIGRITSGTKSPSLAKSIALGYVSF
                     EDSEIGNHVDVNIRGRSVAAKIVPVPFYNR"
     gene            complement(1689..2549)
                     /gene="folD"
                     /locus_tag="VFY96_11045"
     CDS             complement(1689..2549)
                     /gene="folD"
                     /locus_tag="VFY96_11045"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEX5607044.1"
                     /translation="MTGIIDGKAVAKEVQKQIKEEVEGLERRWGLAPGLAVVLVGDDA
                     ASHIYVRNKEKACKEVGIKSFEHLLPATILEKELLALVQQLNKDKNVHGILVQLPLPP
                     HIHAEKVLEAISPYKDIDGFHPMSQGRLMLGADGFRPCTPMGIMRLLAAVSCDPKGKN
                     AVVVGRSSIVGKPVALMLLEKHATVTICHSRTVSLRDEVGRADILVVAIGKAGLVRGD
                     WIKPGAVVIDVGVNRLPSGKLSGDVEFETAKTRASAITPVPGGVGPMTICMLLYNTLK
                     AAKYSLQRDH"
BASE COUNT          581 a          820 c          680 g          660 t
ORIGIN      
        1 aaagagcctc gatcgaatcg ataccgatag cgcgaagcat ctgctcgcga tcggcagagg
       61 tatgaggagt gtagcgcatg ggcccggcta tttctcctcg tcctcttcct cctcttcatc
      121 ggaatcttct tcgtcgtctt cttcttcctc ttcttctttt tgttgggcga cgtattcggc
      181 gtactcctcg gcatccatca gatccttgag atcatcggga tcgctcaagt cgacgcgaat
      241 catccaaccg tcgccgtaag gatcatcatt gatcgtctcc ggattgtccg gcaatacgtc
      301 attgacctcc agcacgcttc cactcaccgg cgcataaaca tcggacactg ctttcaccga
      361 ctcgacggcg ccaaatggat cgtctttgac gattttctcc ccaatttcgg gcagctcgac
      421 atagacgata tcgccgagct cttcctgagc gtactcggtg ataccaatcg tggcggtttt
      481 accctccacc aggacccact cgtgttcctt cgaatatttt agtccgtcgg gaaactccat
      541 tgctgacctc cttagagcgc cttgctgctt gcttagcggt tgtaaaacgg caccgggacg
      601 attttcgccg cgacacttcg gccacggata tttacatcca catggttgcc gatttcggag
      661 tcttcaaatg agacgtaacc aagcgcgatg gattttgcca gtgacggcga cttagtgcca
      721 ctggtaatcc gaccgatggc gcggccctgc ttttgtaatt cataattggc ccgcgcaatt
      781 cccggcccga gcaattccaa accgaccagc ttgcgtccta atccgcgatt tttttgctca
      841 acgagcgctt cgcgcccgga aaatttcggc ttggaaaact tcgtcaccca ttcgagaccg
      901 gcctcgagtg gcgtggtatg atcgtcgagc tcgtgcccat acaagggata ggccttctcc
      961 agcctgagtg tatcgcgcgc accaagtcca gcgggtatca atttgtccga ctctcctgcc
     1021 gagagtagcg cgtcccacaa tcgagctccg gccgtcgcct cgcaataaag ctcgaagcca
     1081 tcttctcctg tataacccgt ccgcgccacc aagcagcgaa tcgccgcgac gtcggcaaaa
     1141 caaaaatgga atgagttcag acccgctaga tcaaagctcg tcagtggcgc gaggatactt
     1201 tgtgcgcgcg gtccttgcaa cgcgagctga caataatgcg cgctgacgtc ttcaacggtg
     1261 acggctgctc cggcagcatg gttttgtatc cagataaaat ccttgtcagt gtttgcggcg
     1321 ttgacgcaaa tcagaaacgt gtccgctgcc aaacgataaa aaatcacgtc atcgacgatg
     1381 ccgccgcgct cgttcagcag cagattatac tgcgcttgac cgaccttcat tcgcgaaacg
     1441 tcattggcgg tgattcgctg actgaattcg agggcgccgg cgccgcgcaa ttcaatttcc
     1501 cccatgtggc tcacgtcgaa gagtcccgcg tggcggcgca ctgccaaatg ctcctgaatt
     1561 acgccttgat actggaccgg catttcccag ccggcaaatt ccaccatttt tgcggccgct
     1621 cggcgatgcg ccgcgtaaag cggagttctt tttagtcccg gcgccacaat agcgatacga
     1681 acgttggatc aatgatctcg ctgcagggag tatttagccg ccttgagcgt gttgtaaagc
     1741 agcatgcaga tagtcatagg gccgacaccg cccgggaccg gcgtaattgc ggaagcgcgg
     1801 gtcttcgccg tctcgaattc gacatcgccg gataattttc cgctcggcaa tcgattcacg
     1861 cccacgtcga tcacgactgc tcccggcttg atccaatcac cgcggactag ccccgctttg
     1921 ccgatcgcca ccaccaggat gtcagcacga ccgacttcat cgcgcaggct aaccgttctc
     1981 gagtgacaaa tagtcaccgt ggcgtgcttc tcgagcagca tgagcgcgac cggcttgccg
     2041 acaatactgc tgcgcccgac caccaccgcg ttcttgcctt tggggtcgca actcaccgcc
     2101 gccaagagtc tcatgatgcc catgggagta catggccgga aaccgtcagc gccgagcatc
     2161 agccttccct ggctcatcgg gtgaaatcca tcgatgtctt tgtagggcga aatcgcttcg
     2221 agaacttttt ccgcatggat gtgaggcggc agcggcaact gcaccagaat accatggacg
     2281 ttcttatctt tgttcagctg ctggactagc gcgagcaatt ctttttccaa gatcgtcgct
     2341 ggcagcaaat gctcgaagga tttgataccg acttctttgc aggctttttc tttgttgcgc
     2401 acgtagatat gcgacgcggc gtcatctccg accaagacca ccgccaatcc cggcgccaat
     2461 ccccagcgcc gctcgagacc ttcgacttct tctttgattt gtttttggac ttctttcgcg
     2521 acggctttac cgtcgatgat cccggtcatg acttgctccg cgacaagatt gaaaatctat
     2581 accggaaaaa atcagcttgt aaaggactga tacgttcatt gcatgacacg gtgctgttta
     2641 aacgtcaaca cctttgtcgc gaggcaaaaa caaaattgtt ttgatccgtg acgccgtttc
     2701 atttgacaat cccgcatgca tcctttaagg cgagaatcac g
//
LOCUS       DASULZ010000540         5209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_164826, whole genome shotgun sequence.
ACCESSION   DASULZ010000540 DASULZ010000000
VERSION     DASULZ010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164826"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VFY96_11050"
     CDS             complement(<1..656)
                     /locus_tag="VFY96_11050"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5607045.1"
                     /translation="MTSIDQTSHRVGGALALLDRHRLAAAIREFRERTLGLVADLDEK
                     QMIGPRLAIVNPPLWEIGHIAWFTEFWLLRHLKKQKPLLPNGDQLYNSSDVAHDTRWE
                     LLLPSRDRTLNYMGAVLDRALERLDGSRELNADERYFYLLTLFHEAMHDEALVYTRQT
                     LAYPPPQFTPVDESCRLTGAVCTGDVDIPGGKFIIGATQDFPFVFDNEKWAHEIELRP
                     "
     gene            complement(779..1939)
                     /locus_tag="VFY96_11055"
     CDS             complement(779..1939)
                     /locus_tag="VFY96_11055"
                     /inference="COORDINATES: protein motif:HMM:NF019193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase domain-containing
                     protein"
                     /protein_id="HEX5607046.1"
                     /translation="MRVTHENFLRRGVVEGFFGPPWSLAERRLLFDFGADRGMNTYLY
                     APKDDPYHRLRWRDPYPEREWRKLLRLMAAAHSKDIDFVYGFHPGEHLCFSERKPVQI
                     LIDKAKRFYDAGVHTFAVLFDDIPSRLVYARDRRTFAGSLARAQGSWLAEIHARQPQS
                     WKHVEWWICPSYYSEDPLLERVFGRFESNFLETLAQDLPANVACLWTGPAVVSGKITL
                     EHARQIARRVRHPLLLWDNYPVNDLSMASELHIGPLTGRDRRLPEIVYGYLNNPMLQP
                     TLSLVPLATCFDYAARPRNYDPETSWRTVIKQRFGADTIKYWQTIGWFLETSQQLIKA
                     KSSMRIETREIARLHSVLTYLHAASKQKWGREIRPWRAQIEAALQNGAVGLS"
     gene            2083..3270
                     /locus_tag="VFY96_11060"
     CDS             2083..3270
                     /locus_tag="VFY96_11060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5607047.1"
                     /translation="MKIGLAHKRFELRGGTERVFYRTAVGLRDRGHDVHLFCLKFSIP
                     PPTGITPHRVPGVTWPRTGRLLSFGIFAPLAISRADCDVVLSFDRLFKQDLFRSGGGP
                     HKTFIEKMQRNGGWKSLWYRVSPYHRLALCIEQRQITSGSRRIVAVCEQSKREMIQAY
                     GIAEKKIVVILNGVDHEKFNPSRRQDIGERTRRELGIQSNARVVLFVGTGFRRKGLDR
                     LLRLWRAGAMPNTYLLVVGSDSRLAAYRATWRQNPYVIFAGPKPNVEDYYAAADVLAL
                     PSIQEAFGNVVLEGLAAGLPVITVAGVGAFDKIEGDLRSGILENPDDLVELKDKLLFF
                     LEPTRWSSLSRAARKMAQQYSWTTYLDQLEETLLSLYEQSAATTIAPEVAIPTFSGTP
                     PSH"
     gene            3280..4263
                     /locus_tag="VFY96_11065"
     CDS             3280..4263
                     /locus_tag="VFY96_11065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5607048.1"
                     /translation="MEPAVSILIATQGRVPQLKRLLDSLVGLENCAEIPHEVLVANNA
                     QNQATATAVEALLQKYRADEPERWVQVRESIPGKSRALNRLLPLAKANLLAFLDDDVE
                     VAPEWLRITWLFFRDYSYDVMQGSILVPPSMQKDEQFLRLLNRYRTICYYSKPGLEVR
                     QIVSLNAANMAFRKELLDATGLFDERIGPGASGTSMDVEFGERVLRAGRRIGYEPRSV
                     VYHDVDWSRLTEGYFKQRHELQGRSRAIYKGSGVLSSLANLLRAAFGFCLYTIIGNER
                     KIYRSKGRIYHYRAMLKEASRTVLGPLLSAPAIPSRPPVLDEREARESKSS"
     gene            4244..>5209
                     /locus_tag="VFY96_11070"
     CDS             4244..>5209
                     /locus_tag="VFY96_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607049.1"
                     /translation="MSRNLRSSTVAIGNDDRETKPLVDSQSHLGRRFRVMYILKNYPQ
                     ISETYIKTELEAVREICDVRIIATKRANMPAKNHEPYRYIEDPAVICEAIEEFQPDVL
                     HSHWFHSVRLLGKLARKTGVPFTVRGHSFDSIWPEDESPFRHFPLLSVFARPSHIRRA
                     IKFIENEFCLGLLTLPFARPRLERAGFPGAKLVDCFPVVNFKRFFDPRPNGTAIMNVG
                     ACIPKKRMEDFVELALRAPELDFNLYALGYKVAELQEFARARQSPVRFIPPVELEEMP
                     AQYKKHRWLVYTAAPGGNVGWPLAVAEAQASGVGVCMANIRPDLRE"
BASE COUNT         1173 a         1393 c         1470 g         1173 t
ORIGIN      
        1 aagggccgca gctcgatctc atgtgcccat ttttcgttgt caaagacaaa agggaagtcc
       61 tgagtagcgc cgataatgaa tttgcccccg ggaatgtcga cgtcgccggt gcacacggcg
      121 ccagtcaacc ggcagctttc atcgaccggc gtgaattgcg gcgggggata agctagggtc
      181 tgacgtgtat aaaccaaggc ttcgtcatgc atcgcttcgt gaaacagcgt gagcagataa
      241 aaatagcgct catcggcgtt tagttctctc gaaccatcga gccgttctag cgctcggtcg
      301 agcactgcgc ccatgtagtt gagggttcga tcgcgcgacg gcaataaaag ctcccaccgg
      361 gtgtcgtgcg caacgtcgct cgaattatag agttgatcgc cattgggcaa cagcggcttt
      421 tgttttttca gatgacggag cagccaaaat tctgtgaacc aggcgatatg gccaatttcc
      481 caaagcggcg gattgacgat cgctaggcgt ggcccgatca tttgtttttc gtcgaggtcg
      541 gcgacaagtc caagggtgcg ttcgcgaaac tcgcggatcg cagcggccaa ccgatgccgg
      601 tccaataaag caagtgcgcc gccgacacgg tggctggttt gatcgatgct cgtcaaaatg
      661 agttatccgg ctctttctgg gtgaattcac gcgcgcagcg atgaacggac tggccgcatc
      721 tcgtgacaat tttacgcttc cttgcgctta tctgtgactg tttagcgcaa agcatcggct
      781 atgacaatcc gactgcgcca ttttggagcg ccgcttcgat ttgtgcgcgc cacgggcgaa
      841 tctcgcgtcc ccatttctgc ttgctcgccg cgtgcagata ggtgagtacc gagtgtaacc
      901 gcgcaatctc tcgcgtctca attcgcatcg atgatttcgc tttgatcagc tgctgtgatg
      961 tttcgagaaa ccatccgatg gtttgccaat atttaatggt gtcggcgccg aagcgctgtt
     1021 tgatgacggt gcgccaactt gtttccggat cgtagttgcg cggtcttgca gcatagtcga
     1081 aacaggtcgc cagcggcacc aaactcaagg tcggctggag catcggattg ttcaaatagc
     1141 catagacgat ttccggtagg cgccgatctc ggcccgtgag cggtccgatg tgcaactcgg
     1201 aagccatcga tagatcatta accgggtaat tgtcccataa cagcagggga tgacggacac
     1261 ggcgtgcaat ttgccgcgca tgctctagcg tgattttacc ggacaccact gccggccccg
     1321 tccaaagaca ggcgacattt gctggcagat cttgagccag tgtttccagg aaattggatt
     1381 cgaaacggcc gaacacccgt tcaagcaacg gatcttcgct gtaataggat ggacagatcc
     1441 accattcgac atgtttccac gactgtggtt ggcgcgcgtg gatttcagca agccacgacc
     1501 cctgggcgcg agccaaactt ccagcgaacg tgcgccggtc gcgagcgtac acaagccgtg
     1561 aaggaatatc atcgaagagc actgcgaatg tatgcacacc ggcatcgtaa aaccgcttgg
     1621 ccttatcgat taggatctga accggcttgc gctcggaaaa acataaatgc tcgccaggat
     1681 ggaagccgta gacaaaatct atgtctttgc tatgagcagc ggccatcagc cggagcagct
     1741 ttcgccactc gcgttcggga tagggatcac gccagcgcag ccggtgatac ggatcatcct
     1801 tgggcgcgta aagataagtg ttcatgcctc ggtccgcgcc aaagtcgaag agaagccggc
     1861 gttcggccag agaccacggc ggaccaaaaa atccttcgac aacgccgcga cgaagaaaat
     1921 tctcgtgggt tactcgcatc aacagttact ttttagcgga ttaactggag gaaacacaat
     1981 tcgacatgac cgactcacag cactgcattt cggtcatggc cgcttgattg tctttacaaa
     2041 ctccacttga aaggagtata gggcggcagt agggtaaact agatgaagat cggcttggcg
     2101 cacaagcgct ttgagcttcg cggtggcacc gaacgggtat tctaccggac tgcggttggc
     2161 ttgcgtgatc gcggccacga tgtgcatctg ttctgtttaa aattttctat tccgccgccc
     2221 acagggataa caccccatcg tgtgccggga gtgacatggc cgagaaccgg gcgtttatta
     2281 agcttcggta ttttcgctcc attggcgatc agccgcgcag attgcgatgt ggtcctgagc
     2341 ttcgatcgat tattcaagca ggacttgttt cggagcggtg gtggaccgca caagactttc
     2401 atcgagaaaa tgcagcgaaa cggcggctgg aaatcgctct ggtaccgcgt gagtccctac
     2461 catcgcctcg ctttgtgtat tgaacaacga cagatcacat cgggaagccg gcgcatcgtc
     2521 gccgtgtgtg agcaaagcaa acgtgagatg attcaagctt acggcatcgc ggaaaaaaag
     2581 attgttgtca ttctcaatgg agtggatcac gaaaaattta atccaagccg cagacaggac
     2641 ataggagagc gtacgcgtcg tgaactaggg attcaaagta acgcccgagt cgtcctgttc
     2701 gtcggaaccg gattccggcg aaaaggattg gaccgcctgc ttcgattgtg gcgcgccggc
     2761 gctatgccta atacctatct cttggtggtg ggcagcgata gtcgtttggc cgcttaccga
     2821 gccacttggc gccagaaccc gtacgttatt ttcgccggac ccaagccgaa cgtggaggac
     2881 tattatgccg ctgccgatgt gctcgctctt ccatcgattc aagaagcctt cggcaatgtt
     2941 gtattggagg gattggcggc gggactgccg gtgatcacgg tagctggcgt gggcgccttc
     3001 gataaaatcg aaggggatct caggagcgga attctggaaa accccgacga cctggtcgaa
     3061 ttgaaagata aactgttatt ttttctggaa cctacccggt ggagttcact ttcacgtgcc
     3121 gcgcgcaaaa tggcgcaaca atatagctgg acgacgtacc tcgatcagtt ggaagaaacg
     3181 ctgctcagct tgtatgaaca gtctgcggcc acaacgatag ctccggaggt tgcgattccg
     3241 acgttctccg gtaccccgcc gagccattaa tttatctcga tggagcccgc ggtcagcatc
     3301 ttaatcgcca cccaagggcg agtgccacag ctgaagcgac tgctcgacag cttggtaggg
     3361 ttggagaatt gtgcagagat tccgcacgaa gttctcgttg ccaataacgc tcaaaatcaa
     3421 gctaccgcca cggcggtaga agctctactg caaaaatatc gagctgatga gccggagcga
     3481 tgggttcagg tgcgcgaatc catcccgggg aaatcacgcg ctctcaatcg cctgttaccg
     3541 ctcgccaaag ccaatcttct cgccttcctc gacgatgatg tggaagtcgc gccggaatgg
     3601 ttgcgaatca cctggctatt ctttcgcgac tattcatacg acgttatgca gggatcgatc
     3661 ctcgtcccgc cgtcgatgca aaaagacgag cagtttctac gtctactgaa tcgctatcgg
     3721 acaatctgct attacagtaa acctggactt gaagtccgac agatcgtcag tctgaacgcc
     3781 gccaatatgg ctttccgaaa agaattgctg gacgcgaccg gcttattcga tgaacggatc
     3841 ggtccgggcg cctcgggtac atcgatggat gtcgagtttg gggaaagggt tctgcgggcc
     3901 gggagacgca tcggttacga gcctcgctcg gtggtttatc atgacgtcga ctggagtcgg
     3961 ctgaccgaag gttattttaa acaacgtcac gagctgcagg gacgcagccg cgcgatttac
     4021 aaaggcagcg gggtgttgtc gagccttgcg aatttgcttc gggcagcgtt cggtttttgc
     4081 ttgtacacga ttatagggaa tgaaagaaag atctatcgct cgaagggacg gatttatcac
     4141 taccgcgcga tgttgaagga ggcgagtcgg acggtgctgg gaccgctgct ttcggcacct
     4201 gcaattccct cacggccgcc ggtcctcgac gagagagaag ctcgtgagtc gaaatcttcg
     4261 tagctctacg gttgccatcg gcaacgacga ccgcgaaacc aaaccgctgg ttgattcgca
     4321 gtcccatctc ggacgcagat ttcgggttat gtacattcta aaaaattacc cgcaaatctc
     4381 cgagacctat atcaaaaccg agctcgaggc ggtgcgtgag atttgcgacg tccgcatcat
     4441 cgcgaccaaa cgggccaata tgccggcaaa gaatcacgaa ccgtaccgct acattgaaga
     4501 tccggccgtg atttgcgaag caattgagga gtttcagccg gatgtgttgc atagccactg
     4561 gtttcattcg gtgcggctcc tgggtaaact ggcgcgcaaa accggcgtac cgttcacggt
     4621 gcgcggccat tccttcgatt ccatctggcc tgaagacgaa tcgccattcc gccattttcc
     4681 gctgctctcg gtatttgcgc ggccctcaca cattcggcgg gcgataaagt tcattgaaaa
     4741 tgagttctgt ctcggcttgc tgaccttgcc atttgcccgt ccgcgactgg aacgcgcggg
     4801 tttccccggc gcaaagctcg tcgattgctt cccggtagta aactttaagc gctttttcga
     4861 tccgcgccct aacggcaccg cgatcatgaa tgtcggcgca tgcattccca aaaagcgtat
     4921 ggaggatttt gtcgagcttg cgctgcgcgc gccggagctc gacttcaatc tttacgcgct
     4981 cggctacaag gtcgctgagc tccaggaatt cgcgcgcgcg cgtcaaagcc cggtgcgctt
     5041 cattcctccg gtagagctcg aagagatgcc ggcgcagtac aaaaagcatc gatggctcgt
     5101 ctacaccgct gcacccggag gcaacgtcgg atggccgcta gcggtcgccg aagcgcaagc
     5161 ttcgggcgtt ggcgtctgca tggccaatat tcgtcccgac cttcgggaa
//
LOCUS       DASULZ010000541         5015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1483946, whole genome shotgun sequence.
ACCESSION   DASULZ010000541 DASULZ010000000
VERSION     DASULZ010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483946"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /gene="radC"
                     /locus_tag="VFY96_11075"
     CDS             complement(<1..568)
                     /gene="radC"
                     /locus_tag="VFY96_11075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEX5607050.1"
                     /translation="MASYQTIKNWPQADRPREKLLAQGSRVLSEAELLAIILRNGNAS
                     TGESAMDHARVLLGQFGGLQGIEAASVGELSAVKGIGPAKVAQIKASLEIGRRMGTRK
                     WETGQPLRSSEDVYRHFRDHLGDEKRELFYVVLLNNKNRKIREVKISEGSLTASLVHP
                     REVYNPVIRDSAAAVIFVHNHPSGDPAPS"
     gene            complement(640..1815)
                     /locus_tag="VFY96_11080"
     CDS             complement(640..1815)
                     /locus_tag="VFY96_11080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEX5607051.1"
                     /translation="MLSIYLKAGRERPVLNGHPWVFSGAIERVQGDGKSPEVVDVYDH
                     KKTWLARGFYNPQSQIRARLLTWRKEEIDRGFFSRRLSYALAFRRAHLPPSTTAYRII
                     NGEGDFLPGLTIDRYGGFLVCQIFSAGMALFKNDIVDYLSRLDSIEGVFERSEGRIGD
                     EEGLEASVGVLSGAAPPDLIPIQENTVKFLVDVRRGQKTGFFLDQRDNRAFVSTIARE
                     KTVLNCFAYSGAFSVYAFAGGAREVVTLDSSLPALELAEKSLQLNGFGNCPGEILKGD
                     AFAYMKEIERKFDLIVLDPPSLAHRRRDTTTAAGGYKFLNLHALKHLNPGGLLLTFSC
                     SQHVPLELFQKIVFGAAVDAGRKVAVIKRLGQPLDHPVSLHHPEGEYLKGFALRALD"
     gene            complement(1890..2369)
                     /gene="moaC"
                     /locus_tag="VFY96_11085"
     CDS             complement(1890..2369)
                     /gene="moaC"
                     /locus_tag="VFY96_11085"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018213758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX5607052.1"
                     /translation="MSQLSHLDEQGRARMVDVSGKEITSRIAIARGTIHMRAETLALI
                     LEDKIEKGDVFAVARVAGIMAAKKTAELVPMCHPLNITAVEIELTAGKDPARVDIEAT
                     VRVTGKTGVEMEAMTAVSVAGLTIYDMCKAVDREMTIGDIRLAKKSGGKSGTFIRKE"
     gene            2479..3420
                     /gene="trxB"
                     /locus_tag="VFY96_11090"
     CDS             2479..3420
                     /gene="trxB"
                     /locus_tag="VFY96_11090"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX5607053.1"
                     /translation="MDKKSHYRVTILGSGAAGLTAAIYAARANLEPLVIEGSQPGGQL
                     TITTDVENYPGFPNGILGPELMEEFKKQAVRFGTKTLFGEVTAVELRKRPFSLAVDKE
                     IVTCDALIIATGASAKLLGLESEMRLMGHGVSACATCDGFFFREKELVVVGGGDTAME
                     EAIFLTKFATKVTVVHRRDQLRASKIMQERAEKNPKISFIWNTVVDEVYGDAKAGGVT
                     GVRFKNIVNGRTEDFKTNGVFIAIGHEPNSKLFVGQLELNPSGYILTHDGTKTNIPGV
                     FACGDVQDFIYRQAITAAGSGCMAAIDAERFLESQHH"
     gene            complement(3518..4156)
                     /locus_tag="VFY96_11095"
     CDS             complement(3518..4156)
                     /locus_tag="VFY96_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEX5607054.1"
                     /translation="MNVNELNISKTALLFFDMLNIYYHGASQETKKRMKPVVDNAVRL
                     MNAARQNRMLIYYAMANHRPDGESRSLAVTDTDMRLRPWPNNDCNPTLHGATEGSWEQ
                     KVIEEIAPKPEDYVIPKYRWSTFHQTYFDLALRSRGIDTLIISGGAVDVGVASTVYAA
                     RDLDYSVIVVRDACSNSHEDSMAAFMNTVFPRMGRVRTTEQVLGMIRTATSA"
     gene            complement(4250..>5015)
                     /locus_tag="VFY96_11100"
     CDS             complement(4250..>5015)
                     /locus_tag="VFY96_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5607055.1"
                     /translation="NGLATGVVLKSGEEIRAERIVSSVDVKQTLLGLIEATALNPHFL
                     LQVKNIRSRGTVAKINLALDGLPKFSSVDQAVLGGMIHIGPSLDYLERASDDAKYGRF
                     SKRPFLEITIPSIADPSLAPAGKHIMSVWMQSAPYALRESNWYAEREALGDAVLQTIE
                     EYAPGFLNRILHRQVLTPLDLEETYGLTQGHPYHAEMALDQIFFMRPIPGWAAYQTPI
                     NNLYLCGSGTHPGGGITGLPGYYAAREVLKNWRSKK"
BASE COUNT         1137 a         1337 c         1394 g         1147 t
ORIGIN      
        1 ggctcggcgc cggatcgccg ctcggatgat tatgaacgaa gataacggca gcggcggaat
       61 cgcgaatcac cggattatag acctcgcgcg ggtgaactag agaagccgtc agcgatccct
      121 cggaaatttt aacctcacga atcttgcgat tcttgttgtt gagcagcacg acgtaaaaaa
      181 gctcgcgttt ttcgtcgccg agatgatcgc gaaagtgtcg atagacatct tccgaggagc
      241 gaagcggctg gcccgtttcc catttgcgag tacccatgcg ccggccgatt tccagagagg
      301 ctttgatctg cgccaccttc gcgggcccaa tccctttaac cgcgcttaat tcgccgaccg
      361 aagctgcttc gatgccctgc agtccgccga actgaccgag cagtacacgg gcgtgatcca
      421 tcgcgctctc accggtactc gcattgccgt tgcgtaaaat gattgccagg agttccgctt
      481 cgctcagtac gcgcgaaccc tgcgccagga gtttttccct tgggcgatcg gcctggggcc
      541 aattcttaat tgtctgataa gaggccatat tttcctcgtt gtatgtatca ttggggttat
      601 ggaccgaccg aggacacggt ctagcacagc agcgatgacc tagtccaatg cgcgaagcgc
      661 gaaccctttc agatattcgc cttcggggtg atgcaggctt acggggtggt ctagcggctg
      721 cccgagtcgc ttgatgaccg cgactttgcg tccagcatcg acggcggcac caaaaacaat
      781 tttttgaaaa agctccaacg gcacgtgctg tgagcaggaa aaggtcagga gcaaaccgcc
      841 gggattgagg tgcttcaagg catggagatt taaaaatttg taaccaccgg ctgccgttgt
      901 ggtatcgcgg cgccggtgcg ccagggacgg cgggtccagg acaatgaggt cgaatttgcg
      961 ttcgatctct ttcatatatg caaaagcatc tcccttcaaa atttctcctg gacagttgcc
     1021 gaaaccattg agctgcagcg acttttcggc gagctcaagc gcggggagcg aagagtcgag
     1081 ggtgaccact tcacgggcgc cgccggcgaa agcgtagacg gaaaacgcgc cgctgtaggc
     1141 gaaacagtta agcaccgttt tctcgcgcgc aatggtcgaa acaaaagcgc gattatcacg
     1201 ctggtcgaga aaaaaaccag ttttctggcc gcgtctcaca tcgacaagaa atttgacagt
     1261 gttttcctgg atcggaatta agtccggcgg cgctgcgccg ctcagcacgc cgacggacgc
     1321 ttcgaggcct tcttcgtcgc caatgcggcc ttcgctccgc tcgaacacgc cttcgatgga
     1381 atcgagcctg ctcaggtaat cgactatgtc atttttaaaa agcgccatgc cagcgctaaa
     1441 aatctgacac acaagaaacc cgccgtaacg atcgatggtg aggccgggca aaaaatcgcc
     1501 ttcgccgttg atgatccggt aggccgttgt cgagggcggc aggtgggcgc gccgaaaggc
     1561 cagggcgtag gaaagccgcc gcgaaaaaaa accgcggtcg atctcttctt tgcgccaggt
     1621 taaaagccgc gcgcggatct gtgactgcgg attgtaaaaa cctcgagcca gccatgtctt
     1681 cttgtgatca taaacatcga caacttcggg ggatttgcca tcgccttgaa cacgctcgat
     1741 ggcgccggaa aaaacccatg gatggccgtt gagcaccggc cgctcgcgtc cggctttgag
     1801 ataaatcgac aacatgggaa acggattgcc ttaaaagaca ggggcgcagc atgctgcgcc
     1861 cctgagaaat ggctggcacc cgagcttgcc tattcttttc ttatgaacgt gccgcttttt
     1921 ccaccgctct ttttagcgag acgaatatca ccgattgtca tctcgcgatc gactgctttg
     1981 cacatatcat aaatggtaag gccggcaacg gagactgcgg tcatcgcttc catttcgacg
     2041 ccggtttttc cggtcactcg gaccgtggcc tcgatgtcca cgcgggcggg gtcttttccg
     2101 gcagttaatt cgatttcaac tgcggtgatg ttgagcgggt ggcacatggg aaccaattcg
     2161 gcagtttttt tcgccgccat aatgccagcg acccgagcga ccgcgaaaac gtcgcccttt
     2221 tcaattttgt cttcgaggat aagagcgaga gtttccgctc gcatgtgaat ggtgccgcgg
     2281 gcgatggcga tccggctggt aatttctttt ccgctcacgt cgaccatgcg tgcgcgtccc
     2341 tgttcatcca aatgacttag ctgtgacata acacctgctt tcaagattgc cgctgaaaga
     2401 accattccat gttgtaacag taaaattgct gaaggtagct tcctttctgc ataggcgcat
     2461 ttatgttaca aaaagcaaat ggataaaaaa agccactata gagtgactat cctgggttct
     2521 ggagcggcgg gcctcacggc agcgatctat gccgcgcgcg ccaatctcga gccgttggtg
     2581 attgagggat cgcaacccgg cgggcagcta acgattacca ccgatgtgga aaactatccg
     2641 ggttttccca acgggattct tggccccgag ctcatggagg agttcaaaaa acaggccgtt
     2701 cgcttcggca cgaagactct gttcggtgag gtcaccgccg tcgagcttag aaaacgcccc
     2761 ttcagtttgg ccgtggacaa ggagattgtg acctgtgacg ctctgatcat tgccaccggc
     2821 gcctccgcga aactgctcgg tctcgaatcg gagatgcgcc tcatggggca tggcgtctcg
     2881 gcatgtgcga cttgcgacgg attttttttc cgggaaaaag aactggtggt ggtcggcggc
     2941 ggcgatacgg caatggaaga agcgatcttt ctcacgaaat tcgctaccaa agtgaccgtg
     3001 gttcatcgtc gggatcagtt gcgcgcctct aaaatcatgc aggagcgggc ggaaaaaaat
     3061 cccaagattt cgtttatctg gaataccgtg gtcgacgaag tctacggcga cgcgaaagcc
     3121 gggggtgtca ctggcgtgcg gttcaagaat atagtcaatg ggaggaccga ggattttaaa
     3181 accaacgggg tttttatcgc aatcggtcat gagcccaaca gcaagctctt tgtcggccag
     3241 ttggaactaa atccaagtgg ctacatcctt actcatgacg gcaccaaaac caatattcct
     3301 ggtgttttcg cctgcggcga cgtgcaggat ttcatttacc gccaggcgat cacagcggcc
     3361 ggtagcggct gcatggccgc catcgatgcc gagaggtttt tggagagcca acatcattga
     3421 atttactgac ccacgaaatt tagttccggt tagatctaaa gataacaaaa cggctccgag
     3481 cagacacgac aacaattatt cgcagcagct ttaattttta tgcgctggtt gcggttcgaa
     3541 tcatgccgag cacctgctcg gtggttctga cgcggcccat acgcggaaac accgtattca
     3601 tgaaggcggc cattgaatcc tcatgagagt tgctgcaggc gtcgcgtacc acgatgacac
     3661 tgtaatcgag gtcacgtgcg gcatatacgg ttgatgcgac accgacgtcc accgctccgc
     3721 ccgaaatgat cagagtatcg atgccgcgtg agcgaagcgc caggtcaaaa taggtctgat
     3781 gaaacgtgct ccagcgatac ttggggatga cgtaatcttc aggcttgggc gcgatttctt
     3841 cgatgacttt ttgctcccac gatccttcag tggcgccgtg tagagtagga ttgcagtcat
     3901 tgttgggcca cgggcgaagg cgcatatcag tgtcagtaac cgcgaggctt cggctctcgc
     3961 cgtcgggtct gtggttcgcc atcgcataat agatgagcat ccggttttgg cgcgccgcat
     4021 tcatcagacg aacagcgttg tcgacaacgg gcttcattct ttttttcgtt tcctgcgaag
     4081 cgccgtggta gtaaatattc agcatgtcaa agaagagtag cgcggttttg ctgatattga
     4141 gttcattgac gttcatcgag gccccctcgt tttaagtcgc cgttcatctt gaccacggcg
     4201 atttctcacc agtgcctatc acgcggtcgc accgattccc agacgaaaat cacttttttg
     4261 aacgccagtt tttaagaact tctcgagcgg catagtaacc gggaagtcca gtgatgccgc
     4321 cgcccggatg tgtgcccgag ccgcagagat agagattgtt gatcggcgtt tggtaggctg
     4381 cccagccggg aatcggtcgc atgaagaaga tttgatccag cgccatttcg gcatggtacg
     4441 ggtggccctg cgtcagtccg taggtctctt cgagatcaag cggtgttagc acctgccgat
     4501 ggagaattct gtttaagaaa cccggcgcgt actcttcaat cgtttgaaga accgcatcgc
     4561 ccaaagcttc gcgctcggca taccaattac tttcccgaag cgcgtacggc gctgactgca
     4621 tccagacgga catgatatgc ttgccggcgg gagcgagcga cggatcggcg atcgacggaa
     4681 ttgtgatttc caagaacggc cgcttcgaaa accggccata cttcgcatcg tcagaggcac
     4741 gctccagata atccaaactg ggaccgatat ggatcatgcc gccgagcact gcctgatcaa
     4801 ccgaggaaaa cttgggcaag ccatccaaag cgagattgat tttcgcgacg gtaccccggc
     4861 tacgaatgtt tttcacttgt agtaaaaagt gcggattgag agcggttgcc tctatcaggc
     4921 caagaagcgt ttgcttcacg tcgacgctgg agacgatccg ctctgcccga atttcttcgc
     4981 cgctttttag aacaacaccc gtcgctaggc cgttg
//
LOCUS       DASULZ010000542         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1077275, whole genome shotgun sequence.
ACCESSION   DASULZ010000542 DASULZ010000000
VERSION     DASULZ010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077275"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VFY96_11105"
     CDS             <1..476
                     /locus_tag="VFY96_11105"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5607056.1"
                     /translation="WLAGLIDTMGEDRALAFARKLAAQNLRVQQGSTLLVQQMSAGEF
                     KVLIDALHYQIENFKLQGAPVEYVIPDPLLIKDPSGLWLAKHAPHPHAAALFIDFLLS
                     REGQEFFVQSHRLVARKDLEWRSLGKLPERTHVLSVEKWAPRNKQLIAQFDKIFR"
     gene            complement(792..1727)
                     /locus_tag="VFY96_11110"
     CDS             complement(792..1727)
                     /locus_tag="VFY96_11110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018636731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX5607057.1"
                     /translation="MKLEGSDVGEVGIWTSQFDFQPAAQLRNAASDLEKLGFGAIWFP
                     ESVGREAFTHAALILGATKRLVVATGIANIYGRDPVTALAAQKSLAEAYPGRFLLGLG
                     VSHIPLVEQVRGHTYGKPVAAMRAYLDRMDQAPYRAVIPAVKPARVLAALGPRMLQLA
                     AERADGAHSYFVTPEHTAEARKILGNDRFLAVEQAVVLEKDASRAREIARAHTSFYLR
                     LPNYVNNLRRLGFSDDDFGDGGSDRLVDAIVAWGDVGATIDRVRAHQAAGADHVCIQL
                     LPPEPQALPVGEWREIAAALLGSTGRRRSSAASKT"
BASE COUNT          519 a          636 c          614 g          451 t
ORIGIN      
        1 aatggctcgc cggactgatc gacaccatgg gcgaggacag agcactggcg tttgcccgca
       61 agctggccgc acaaaacctg agagtccagc agggcagcac cttgctcgtc caacagatga
      121 gcgcggggga attcaaagtc ctgatcgacg cattacacta tcagatcgaa aattttaagc
      181 tgcaaggcgc gccggtggag tacgtcatcc ccgatccgct gctcataaaa gacccgagcg
      241 gcctgtggct cgcgaagcat gcgccgcatc cgcacgcggc ggctttgttc atcgattttc
      301 ttctgtcgcg tgaaggccaa gaattctttg ttcaatcgca tcggctcgtg gcgcgcaagg
      361 acttggaatg gcgctccctc ggcaaactgc cggaacgcac gcacgtgcta tcggtggaaa
      421 agtgggcgcc gcgaaacaaa cagcttatcg cccagttcga taaaattttc cgctaggaaa
      481 atggaattac gcgagggaat tatggtagac ggataacagt tctgtcagac ttgcctaagt
      541 ctagcaacta taattcttcc acagcccacg gcttaagttg aatggatggc aattaataaa
      601 atggccacct tggaatgcgg atagtcttgt atcaacagag ccaataaacg tttgccagcc
      661 ggctcttgtg aaccaatatt tcggcagaaa gtccgcttcg acaagtttgc ggtcaaatct
      721 ttactttgac attcgcgccg cgaaaggttc aggattcctc aaagcactgg ggtcaagtca
      781 gacagttgag gtcaagtctt gctggcagcg gatgatcgcc gccgcccggt agagccgagc
      841 agtgcggctg cgatttcacg ccactcacca accggcagcg cctgcggctc tggcggcagc
      901 agctgaatgc aaacatgatc ggcgcccgca gcttgatgag cgcgcacacg atcgatcgtt
      961 gcgccgacgt cgccccacgc gacgatggcg tcaactaggc gatcgcttcc gccatcgccg
     1021 aagtcgtcgt cactgaagcc gagccggcgc aaattgttaa cgtagttcgg caggcgcaag
     1081 tagaacgagg tgtgtgcacg cgcgatttcg cgcgctctgc tagcatcttt ctcgagcacc
     1141 accgcctgct caacggcgag aaagcggtcg ttgccgagaa ttttacgcgc ctcagccgtg
     1201 tgttccggcg tgacaaaata agaatgcgcg ccatcggctc gttcggcggc gagctggagc
     1261 atgcgggggc cgagcgctgc cagcacccgc gccggtttta ccgcggggat caccgcgcgg
     1321 taaggcgcct gatccatgcg atccaagtac gcgcgcatgg ccgcgaccgg ttttccgtaa
     1381 gtatgacctc tcacctgttc caccaacgga atatggctga cgcccagacc tagaagaaag
     1441 cggccgggat aggcttccgc aagactcttt tgcgctgcca gcgccgtgac gggatcgcgc
     1501 ccgtagatgt tcgcaatgcc cgtggctacg acgagccgct tggtcgcgcc caaaattaaa
     1561 gcggcgtgag taaaggcttc gcggcccaca ctctccggga accagatcgc gccgaaaccg
     1621 agcttttcaa gatctgacgc cgcgttgcgc agttgtgccg cgggctgaaa gtcgaactgc
     1681 gacgtccaaa tccccacctc gccgacatcg ctgccttcta acttcatact tcctcctctt
     1741 atcgcctcgg gccgcaaacg ttattccgac gagtgaatca tcaccagtct accaaatcac
     1801 gacggcaacg ctatccgcag gagcgcggca gcgactcgaa actcaaatcg ggaaggtgcg
     1861 aaacagcgcg aaaaattgcg ccggagcgat tttcctcgag gcgctcgcgt cattctgact
     1921 accgacagtt attacggtca aaaagaaaaa aggagtggga tcacgcatga gttattgcaa
     1981 cttgagagca cctttttgct gtatttgtga taacatccaa aaaagctctg ttcatgacga
     2041 tgccgtcgag gatctcaccg atgacacccg agctacagcg cgcggtgaag cgctatcaac
     2101 tctggttcgg ctcatacaag agatcgggcg agctcatcaa ggttcaagtt tggctcacga
     2161 tcaataacgg tcagattgaa tttctgaccc ctggtggttc gtacaaagtg aagcgaatcc
//
LOCUS       DASULZ010000543         2750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1180436, whole genome shotgun sequence.
ACCESSION   DASULZ010000543 DASULZ010000000
VERSION     DASULZ010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180436"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(220..1227)
                     /locus_tag="VFY96_11115"
     CDS             complement(220..1227)
                     /locus_tag="VFY96_11115"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5607058.1"
                     /translation="MATSKWRWLVVFALVLLVPDWTWAQGASVEAAKREGRAVIYGTV
                     VPQVMGQIQNGFETKYGIKTEYWRADATKVIDRVLTEWRSGKPGFDVVIGARGPLALG
                     KEERVYAKYSPAAAANFPAKFKDKDGQLIAWRITPVGILYNTELVKATEAPKSLDELL
                     DPKWQGKISMPDPSRHASTAQYLWNLQQVKGDKWMDFAKGLAKQKPMLVESYSTVPNV
                     IVRGEASLGISYIQYTGQTKGPIGFASIEHVFADPSDAALSAKAINANAAKLFIEYLC
                     SAEGQKKVAETGEFVLSPGVYPAIKGADKIVSNLYMMEDPSAETLAKIQGEFRQLFLA
                     K"
     gene            complement(1293..2276)
                     /locus_tag="VFY96_11120"
     CDS             complement(1293..2276)
                     /locus_tag="VFY96_11120"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5607059.1"
                     /translation="MRLGLCFDGFYSIHEMIELARLADEIGMDSIWMSDHLCFRDSLT
                     TSMALLASTRKIKVAAAPMSPYSRSVIISAMSVATLDEFAPGRVIASPGTGNAAALKE
                     AGIESPHPLETMREYVEVLRRLLKGETVQYQGKMIQINGAKMGFVPAVTIPMYITAVR
                     SRMLQLGGEIGDGVLLSAGCAPAYIQKCVGDIGRGAENAGKKLKDVDVAGFVTASVAD
                     DPREAIEANKMFLAYIFRNTHHAENISLGGGKLDQEALAAAVAKRDWEAAKKFISDEV
                     VFAHSVAGTPAECRRQLERFTTGGLTLPVVLPMGTQEARKRVVRMLREVAA"
     gene            complement(2374..>2750)
                     /locus_tag="VFY96_11125"
     CDS             complement(2374..>2750)
                     /locus_tag="VFY96_11125"
                     /inference="COORDINATES: protein motif:HMM:NF014381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HEX5607060.1"
                     /translation="IVQSLGLERLRFVDIFHQAGLTTEGGRDSGSAYQAIHQSEPNKT
                     IKSPASLDHRYIREDVGYGLVPMAEIGSLFGVCTPVMDALITLASTALDVDFRTEGLS
                     LEKMGLAKIQPDELQRILVDGF"
BASE COUNT          598 a          839 c          688 g          625 t
ORIGIN      
        1 ataaacaacg actttgcctt cttttttggc ggcatcgaga ttgcccgttt gggcgaagat
       61 gttcttgggg aacgtcatgg cgccgacaac aaccacggca acgacaaccg accatcgctt
      121 gatgtatcta ttcacaatta cctcctcaga actcgaaaca gcataacaat tagcgacctg
      181 ccctatttaa gtcaataatg cgtccggtgg cggtatgaat catttggcga gaaacagctg
      241 gcgaaattcg ccttggattt tggctagtgt ctcggcgctt gggtcttcca tcatataaag
      301 attcgacacg attttgtcgg cgcctttgat cgccgggtaa acgccggggg agagtacaaa
      361 ttcgccggtc tcggcaactt tcttctgacc ctcagccgag cacaaatatt cgatgaagag
      421 ttttgcggcg ttcgcgttga tcgccttggc gctcagcgcc gcatcgctgg gatcagcgaa
      481 cacatgctcg atggatgcaa acccgatcgg tcctttggtt tgtcccgtgt attgaatgta
      541 gctgatgccg agagaagctt cgccgcgcac gataacgttt ggcacggtgg aataggactc
      601 cacgagcatc ggcttctgct tggccaatcc tttggcaaag tccatccatt tgtcgccttt
      661 cacttgctgc aaattccata aatactgtgc cgtgctcgca tgtcgcgacg ggtccggcat
      721 gctgattttt ccttgccact ttggatcgag cagctcatcg agagatttgg gcgcctcagt
      781 ggccttgacc aattcggtat tgtaaagaat tcccaccggc gtaatgcgcc atgcaatcag
      841 ctgtccgtcc ttgtccttga attttgcggg gaagttcgcc gccgcagccg gactatattt
      901 cgcgtacacc cgctcctctt tgcccagagc gagaggcccc cgagcgccga tcacgacgtc
      961 gaacccgggc tttcccgagc gccattcggt caaaacccga tcgatcacct tggtcgcatc
     1021 ggcgcgccaa tactcggtct tgattccgta cttggtttca aagccatttt ggatttgtcc
     1081 catcacctgc gggaccacgg tgccataaat gaccgccctg ccctctctct tggcggcctc
     1141 gacgctagcc ccctgcgccc aagtccaatc cggcaccaaa aggaccagtg caaaaacgac
     1201 cagccatcgc cacttcgatg tcgccataat cagcctccct catgaaccaa attggctctg
     1261 tttaaagaaa acccaggtct gaaacaagac tttcaggccg ccacctctcg aagcatacgc
     1321 accacccgtt tgcgcgcttc ctgcgtcccc atcggcaaca ccacgggcaa ggtcaacccg
     1381 ccagtcgtaa agcgctcgag ttgccggcgg cactcagcgg gtgtaccggc caccgagtga
     1441 gcgaagacga cctcgtcact gatgaatttt ttcgccgctt cccaatcgcg cttggccacc
     1501 gccgccgcca aggcttcttg atcgagcttg cccccgccga ggcttatgtt ttccgcatgg
     1561 tgggtattgc gaaaaatgta tgcgagaaac attttgttgg cttcgatcgc ttcgcgcgga
     1621 tcatcagcca ccgacgcggt gacaaacccg gcgacatcga cgtcttttaa ttttttgccg
     1681 gcgttttcgg ctcctcggcc gatatcgccg acgcattttt gaatgtacgc cggggcgcaa
     1741 ccggcagaca gcaacacccc gtcaccgatc tctccaccca attggagcat gcgcgagcgc
     1801 accgcagtga tatacatggg aatcgtcacg gcgggcacaa accccatctt cgcgccgttg
     1861 atctgaatca ttttgccctg atattgcacc gtctctcctt tgagcaggcg gcgtagaact
     1921 tcgacgtatt cgcgcatggt ttcgagcggg tgcggcgatt caatgccagc ttccttcaac
     1981 gccgcagcat tgccggtgcc tgggctcgcg atcacgcggc cgggagcaaa ctcatcgagc
     2041 gtagccaccg acatcgccga aataatcacc gagcgggaat atgggctcat gggcgccgcc
     2101 gcaactttaa ttttgcgcgt cgatgcgagc agcgccatgg aggtcgtcag cgaatcgcga
     2161 aagcagagat gatccgacat ccagatcgag tccatgccga tttcgtcggc caatcgagcg
     2221 agctcgatca tttcgtgaat cgaataaaaa ccatcgaagc aaagccctag gcgcatacat
     2281 catctccaag tttttaaata ttgactcgaa accgacattg cgtacgatcg tgtcatcctg
     2341 acatccgcgg cctacacgag cttcgcatgc ccattagaaa ccatccacga ggatcctttg
     2401 caattcatct ggctgaatct tcgcaagccc cattttctcc agactcaagc cttccgtgcg
     2461 aaaatctaca tccaacgcag tcgaagccag cgtgatcagc gcatccatca ccggcgtgca
     2521 cacgccgaac aagctgccga tctccgccat cggaaccagg ccgtagccga cgtcctctct
     2581 aatgtagcgg tgatcgagcg atgccggcga tttgatcgtc ttgtttggct cgctctggtg
     2641 aatcgcctga tacgctgaac cggagtcgcg gcccccctcg gtcgtcaatc ccgcctgatg
     2701 aaaaatatcg acaaaccgaa gccgctccag cccgagcgat tggacgatct
//
LOCUS       DASULZ010000544         4121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_266389, whole genome shotgun sequence.
ACCESSION   DASULZ010000544 DASULZ010000000
VERSION     DASULZ010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266389"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="VFY96_11130"
     CDS             <1..1200
                     /locus_tag="VFY96_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5607061.1"
                     /translation="AAIARVTRDIGIAEELAQDALVAALERWPEEGIPANPAAWLMTA
                     AKRRAIDNLRRGLMLVQKHEEIARELEAQQQWLGEAMDQALDQVIDDDVLRLIFTACH
                     PILAVEGRIALTLRLIGGLTTAEIARAFLVPEKTIGQRIFRAKKTLSEAHVPFETPRG
                     DELRRRVTSVLSVVYLIFNEGYTATSGEEWMRAALCDEALWLGRMLAQFLPGESEGHA
                     LLALMELNAARTAARRGSNGEAVLLPDQDRSLWDDDHIQRGMSALEHAQRLGGGAGAY
                     ALQAAIAACHMRARTAEETDWERIVLLYDALMQTTPSPIVALNRAVAVSMAHGPAAGL
                     DALDAIAQALVGYHLLHSVRGDLLMKMGNFPEAREEIERAIAMTKNQREQELLTEKLK
                     QIVLRNP"
     gene            complement(1197..2612)
                     /locus_tag="VFY96_11135"
     CDS             complement(1197..2612)
                     /locus_tag="VFY96_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5607062.1"
                     /translation="MRIAVISSYTHPSRLIIKEKSVMQSAVPELIAALCPDDAEIEIY
                     NEKEAYIPLDRDWDLVFFSYLHSYYEHTKVLSTLFRRRGITTVAGGRHARHFPDDCQK
                     YFDAVITGEPESNVPALVRDFERGELKPRYSLPAVEAAAIKPYRYDLIDFRHNRFRLP
                     GVEASRGCPFACNFCVLTGWETYRYRPVKHVIDEIKFKMKWNHHYFGLIKDWFVFLDN
                     NLGGNVKYLRELCEALIPLKKTWGCALTFNVLKDETTVKLMAKAGCRYVYTGLESLNP
                     ESIKAMNKGQNTIREIDNIINKCYANGILLSFGLIVGSDGDTNEYLEKLPGYMADLRY
                     CAITFMGIICPYPETSLFRDFQKEGRLLPGTISRDYDGYTLCHQPKLLDTSEVVDHYR
                     RLSRAIGSLSNVSRHYSSKFWASDMHRYKMTLLVSGPEIFSVRNNLRNKKRSFIAGVD
                     ELEAWDQQKMQELQIQPQYLS"
     gene            3209..>4121
                     /locus_tag="VFY96_11140"
     CDS             3209..>4121
                     /locus_tag="VFY96_11140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5607063.1"
                     /translation="MRSNIGIYNLHMRAMGGGEKLTLVIAEHLSLAHNVFLFSAEPLD
                     VSSLEQFFNVDLSRVKVICLKTPGSLSRVLGRISGRNVSLHHERQLRALELDLFINNS
                     YASALTCPTTRGIFMCMFPHQAVNNVVNSYSTIVAISRYAADWVWKRWDRHSEILYPP
                     CDDMGPPPSAKEKIILHVGRFIADSIEDERHHKGQDLLLETFKRMRDLHEQGWELHFT
                     GSLSPDGKSRAFANTLTRAAEGFPVFFHFNSASEELRKLYRRAAIYWHATGYGFDADR
                     YPAKQEHFGISTVEAMSAGAVPVVYATG"
BASE COUNT          943 a         1102 c         1130 g          946 t
ORIGIN      
        1 gctgcgattg cgcgcgtcac gcgagatatc ggcattgccg aggaacttgc tcaggacgcg
       61 ctggttgcgg ccctcgagcg ttggcccgaa gaaggtatcc ctgccaatcc cgctgcgtgg
      121 ttgatgacgg cggcaaagcg gcgcgccatc gataatctgc gccgcgggct gatgcttgta
      181 caaaagcacg aagaaattgc tcgggagctt gaagcgcaac agcagtggct cggagaggcg
      241 atggaccagg cactggatca ggttatcgat gatgacgtac tacgcctcat ctttaccgca
      301 tgccatccta tactcgcggt agaaggacgc atcgcgctta cgttgcgttt gatcggcggc
      361 ctcactacgg cggagatcgc ccgagccttt cttgtacccg agaaaacaat aggacagcgg
      421 atctttcggg ccaagaagac tctctcggaa gctcatgtgc cctttgagac gccgcgcggt
      481 gacgagttgc gccgcagggt gacgtcagta ctttctgtgg tctatctgat ctttaatgaa
      541 gggtataccg cgacctcggg agaggagtgg atgcgggccg cactctgcga cgaggcgctc
      601 tggctcggac gcatgctggc gcagtttctt cccggcgagt ctgaaggtca tgcactgctg
      661 gctctgatgg agctcaatgc agcgcgcaca gcggctcgcc gtggaagcaa cggcgaggcg
      721 gtcttgctgc cggatcagga tcgctctttg tgggacgacg accacattca gcgaggaatg
      781 tccgcgctcg aacatgcgca aagacttggt ggcggcgctg gagcctatgc tttgcaggca
      841 gcgattgccg cctgtcacat gagagctcgc acggccgaag agactgactg ggaacggatt
      901 gttcttctct acgacgcact gatgcaaacc acgccctctc ccatcgtggc gctgaaccga
      961 gcagtcgctg tgagtatggc ccacggcccg gccgccgggc ttgatgctct tgatgccata
     1021 gcccaggcac tggtcggcta tcacctgctt cacagtgttc gcggagacct gctgatgaaa
     1081 atgggcaact tccctgaggc gcgtgaggaa atcgagcgcg cgatcgccat gacgaaaaat
     1141 cagcgcgaac aggaattgtt gacggaaaag ttgaagcaga ttgttttgcg aaacccctaa
     1201 ctcagatact gcggttggat ctgcaactcc tgcatcttct gctggtccca cgcctccaac
     1261 tcatccacgc cggcaatgaa gctccgtttc ttgttacgca ggttgttgcg gacgctgaat
     1321 atctccggcc cagacaccag cagggtcatc ttgtagcgat gcatgtcgct cgcccagaac
     1381 ttgcttgaat agtggcgaga cacgtttgaa agcgagccaa tcgcgcgcga cagcctgcgg
     1441 tagtggtcta ctacctcgct cgtgtccaac aacttcggct ggtgacacag cgtgtaaccg
     1501 tcgtagtctc ggctgatggt ccctggcagc agccggccct ccttttggaa atcgcgaaac
     1561 aaggaggtct ccggataagg gcagatgatt cccatgaaag tgatggcgca gtaccgcaga
     1621 tcagccatgt agcccggcag cttttccagg tattcatttg tatcgccatc ggatcccact
     1681 atcaggccaa acgaaagcag gattccattc gcatagcatt tgttaataat gttgtctatt
     1741 tctctgatcg tgttctggcc tttgttcatg gccttgattg actcgggatt aagcgactcg
     1801 agtccggtgt aaacgtaacg acagcctgcc ttcgccatca gcttgaccgt cgtctcatct
     1861 ttcaaaacat taaacgtaag cgcacagccc caggtcttct tgagcggtat caatgcctca
     1921 cacagttccc gcaaatattt cacgtttccc ccgagattat tatcgaggaa aacaaaccag
     1981 tctttgatga gaccgaaata gtggtggttc cacttcatct taaacttgat ctcgtcgatg
     2041 acatgtttga ccggccggta gcgatatgtt tcccatcccg taagcacaca gaaattgcag
     2101 gcgaagggac aaccacgtga agcttcaacg ccgggcagac gaaagcggtt gtggcggaaa
     2161 tcgatgagat cgtagcgata tggtttgatt gccgctgcct ccaccgccgg aagactatag
     2221 cggggtttca actcgccgcg ttcaaaatcg cgaaccagcg ccggaacgtt gctttcgggt
     2281 tcgccggtaa tgacggcgtc gaaatacttc tggcagtcgt cgggaaagtg tctggcatgt
     2341 cgcccgcccg cgacagtggt gatgccgcgt cgtcgaaaca gcgtcgacag aactttcgtg
     2401 tgttcgtagt aagaatgcag ataggagaaa aaaaccaggt cccaatcgcg atcgagcggt
     2461 atatatgctt ccttctcgtt atagatctct atttcggcat catcgggaca gagggccgca
     2521 atcaactccg gaaccgccga ttgcattaca gacttttcct tgatgataag cctgctggga
     2581 tgggtatagc tggagattac tgctatgcgc atgagttgcc tccgcgggaa gtaattagca
     2641 gccaaaagat ttctttgagg ctggtgagcg atgccattaa gaaacggtcc cgagatcagt
     2701 aatagagtcg cgaagaccca gttggctata cgaaaaacta taaatagcga aggctaaaac
     2761 ccgcgttcga agaccgccaa aatgagacgg gcagaatcta tcagaaggag agtgctagca
     2821 caatgaaatg cgcgtcctgt taacggcagt ggtctctctg ggtccgcgcg ctttggggcg
     2881 ccggcccaca gacgctcagt tctggcgcat gtttcgcgct acgtgcgtga tcctcctaat
     2941 cttgaaggcc attgttagca gcaatctcta gcggcctcat ggctccgcag attccccttt
     3001 tattattcgt gggatttaga gccgcatcca agtcgcgtct atcatcgcgc ctgtttcggc
     3061 aaccttgact tgccagacga tgtattcaaa cgcaccagag tttgtcacac gttgacgtgg
     3121 tgcaaccgaa aaggaactag tatagctgat gctgtgtcat caaaaaggct agacccaccg
     3181 cttttgagac aaagtacaat gtgccgggat gagatccaac atcggtatct acaatctaca
     3241 catgcgggca atgggaggag gagagaagct gacgctcgtc atcgccgaac atctgagcct
     3301 ggcccacaac gtctttctat tttccgccga gccactcgat gtttcatcac tcgaacagtt
     3361 ctttaatgtt gatctcagtc gggtgaaagt tatctgcctc aaaacccctg gttcgctttc
     3421 ccgcgtcctc ggcagaataa gcggccgtaa cgtttcactt caccatgaac gtcaactccg
     3481 cgcactcgaa ctcgatctgt ttatcaacaa tagctacgcg agcgcattga cgtgcccaac
     3541 tactcgcggg atcttcatgt gcatgttccc gcatcaagct gtaaacaatg tcgtcaattc
     3601 gtattcgacg attgtcgcta tttctcgtta cgccgcagac tgggtttgga aaaggtggga
     3661 ccggcactct gaaatcctct atccgccgtg cgacgacatg gggccgccgc cgtccgcaaa
     3721 agaaaagatc attctgcacg taggaagatt catcgccgac agtatcgagg atgagcggca
     3781 ccacaaggga caggatcttc tattagagac attcaagcgc atgagggatc ttcacgagca
     3841 gggatgggag cttcatttca ccggcagtct cagccctgac ggaaaatcac gagcgtttgc
     3901 aaacacactc acccgcgccg ccgaaggttt tccggtgttc tttcacttca attcggcgag
     3961 cgaagaattg cggaagctat atcgtcgggc ggcgatttac tggcatgcga ctggttatgg
     4021 gtttgacgcc gatcggtatc ccgctaaaca agaacatttt ggtatttcga ctgttgaagc
     4081 gatgtcggca ggggctgtgc cggtcgttta tgctactggc g
//
LOCUS       DASULZ010000545         2726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1281391, whole genome shotgun sequence.
ACCESSION   DASULZ010000545 DASULZ010000000
VERSION     DASULZ010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281391"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VFY96_11145"
     CDS             <1..481
                     /locus_tag="VFY96_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607064.1"
                     /translation="ATLVSPDNLFEAEQKNLKLSILADLKDLGIYTSASDLSAKRDFL
                     KNQRQRARGFMMAYCEAIWLGKANKSIALASFRKHLREQDPRRLETLYKNYVIDALPL
                     KPYPMEDALQGELENLLSSVPELRAKKAADFIDKSLLSDLERDGLFTQLASRYGKQQ"
     gene            complement(677..2005)
                     /locus_tag="VFY96_11150"
     CDS             complement(677..2005)
                     /locus_tag="VFY96_11150"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5607065.1"
                     /translation="MRPAPSHLSPVHVISNDYFRFSSFISLASSNNSSTKTEKPIRPW
                     SSLLIRDYRLLWIASVLSSLAGQIRNVATLYQVYDISGSAFKLGLTGFLETLPFIVFG
                     LFAGAVADAFDRKKLLIATISLQMLPNLILGILTFTGVTQVWHIYALGLLGAFVEVFN
                     WPARAALIPRLVPQVLLMNAMVVNTMILQTSFLVGPAIGGFLIDHGGLSTAYFASTLL
                     FVPAAIAIVSVRASGKIEGERRRVNFRSIVEGIEFIWIQRIILSLFLLDFAVVLVGFY
                     RPILPIFAADVFKAGASGLGVLYAAPSAGSLLGTTALLMAGDVKRKGVLVIIAAIFFA
                     CALALLGISYQFWMAVGAVALLGFTDSISVAIRRTVVQLLAPDGMLGRAASLITVFAQ
                     TTNGLGALLAGIAAEYCGAPNALLIGSSLCFVMIFGICSAIPQLWRYRSP"
     gene            complement(2032..2313)
                     /locus_tag="VFY96_11155"
     CDS             complement(2032..2313)
                     /locus_tag="VFY96_11155"
                     /inference="COORDINATES: protein motif:HMM:NF039404.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6295 family protein"
                     /protein_id="HEX5607066.1"
                     /translation="MIAHQAKIQGRGKIGQDWFELREANVSYDHPYDMPLEHALNIDF
                     VNEAAGPGARVAVELSVDAARKLVQTIEAVLAQADQRGVLEDKHQAANR"
     gene            complement(2333..>2726)
                     /locus_tag="VFY96_11160"
     CDS             complement(2333..>2726)
                     /locus_tag="VFY96_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5607067.1"
                     /translation="CSGGRHGFLAACRLKGFDALVECWGGRVVQSKEELTPKQPVAPI
                     DYTKDLSCPMIGLFGNDDKSPSPEQVNLHEAELKKHGKNYEFHRYDGAGHGFFYYDRP
                     AYRQEQAVDGWKKLFAFCDKTLKSELRR"
BASE COUNT          698 a          735 c          750 g          543 t
ORIGIN      
        1 cgcgaccctg gtgagtccgg ataatctgtt cgaagcggaa caaaaaaact tgaagctctc
       61 gattttagcg gatctgaagg atctcgggat ctataccagc gccagcgatc tttcggcaaa
      121 gcgcgatttt ctcaagaatc agcggcagcg ggcgcgtgga ttcatgatgg cgtactgtga
      181 agcgatctgg ctgggtaaag caaacaaaag cattgcgctg gcgagctttc ggaagcattt
      241 gcgcgaacag gatccgcgcc ggctggagac gctgtacaag aattacgtca tcgatgcttt
      301 gccgctcaaa ccgtatccga tggaagatgc tctgcaaggc gagctcgaga atcttttgag
      361 cagcgtcccg gaactgcgcg caaaaaaggc cgcggatttt atcgacaaga gcttgttgag
      421 cgacctagaa cgggacggct tgttcactca actcgcgagc cgctacggca aacaacaata
      481 acccgcatga ggacggcagg gcagatttat ttagttgtcg agatcacagt acctgaatta
      541 ttcgtggtgc aagcttaaac gaaaagattt ctcgtggctt cgctcactcg gaatgacaag
      601 gttgtcattt tcgacagaag ggagaacttc cttggacagt ctgtttatgc ttttcttcgg
      661 tctatgaata atccagctag ggagatcggt agcgccaaag ttgagggatc gccgaacaaa
      721 ttccgaaaat catcacgaag caaagcgagc tgccgatgag caatgcgttc ggcgcgccac
      781 aatattccgc agcaattccg gcgagcaaag cgcccaagcc attcgtcgtt tgagcaaaga
      841 ctgtaatcaa gcttgcagct ctgccaagca tgccgtcagg ggcgaggagc tgaaccactg
      901 tgcgccttat cgcaacgctg atggaatcgg tgaagccaag aagcgctacc gctccgaccg
      961 ccatccaaaa ctgatatgaa atccccagca aggccaaagc gcaggcgaaa aagatcgccg
     1021 cgatgatcac gagcacgccc ttgcgtttca cgtcgcctgc catcaacaat gcggtggtgc
     1081 cgagcagaga gccggcggat ggcgcggcat aaaggacgcc gagccccgac gcgccggctt
     1141 tgaaaacatc ggccgcaaaa atcggtagaa tgggtcgata aaatcccacc aacacgacag
     1201 cgaagtcgag caaaaacaaa gaaagaataa ttcgctgaat ccagataaat tcgatgccct
     1261 ccacgatgct tctaaaattg acgcgccgcc gctctccttc gatttttccc gacgctcgaa
     1321 ccgagacgat cgcgatcgca gccggaacaa aaaggagggt actggcgaaa taggccgtgg
     1381 ataaaccgcc atggtcgatc aagaatccac cgatcgccgg accgacaaga aaactagttt
     1441 gtaagatcat cgtgttgacg accatcgcgt tcatcaacaa cacctgaggc accaaccgag
     1501 gaatcagcgc ggcgcgcgcc ggccagttga acacttcgac aaaagccccg agcagtccga
     1561 gcgcataaat gtgccagact tgcgtgaccc cagtaaacgt caggatgcca agaatcaggt
     1621 tcggcagcat ctgcagcgat atggtcgcga tgagaagctt ctttcggtcg aacgcatccg
     1681 cgacggcgcc ggcgaaaaga ccgaacacaa tgaacggaag ggtctccaag aagccggtaa
     1741 gtccaagttt aaatgccgat ccggaaatat cgtagacctg gtagagcgtg gcgacattac
     1801 gtatctgccc ggccagcgag ctcaggacgc tggcaatcca gagcaagcga taatcacgga
     1861 tcaaaagcga tgaccaggga cggatcggct tttctgtttt cgtgctggaa ttgttagagc
     1921 tggccaatga gataaacgag gagaagcgaa agtaatcgtt acttataacg tgaacgggag
     1981 ataaatgcga tggggccggg cgcaaggcag agtgcctcgc gcccgatggt ttcagcgatt
     2041 ggcggcctga tgtttgtctt ccagcacgcc gcgctgatcg gcttgcgcga gcacggcttc
     2101 gatggtctgt accagtttgc gagcggcgtc gacactgagc tcgaccgcga cgcgcgcgcc
     2161 cggtccggcc gcttcgttca cgaagtcgat gttgagcgcg tgttcaagcg gcatatcgta
     2221 aggatgatcg tacgatacgt ttgcttcgcg cagttcgaac caatcctgtc cgatctttcc
     2281 ccggccctga atcttcgctt gatgagcaat cattgtgcac atggtgcgct ccttatcgcc
     2341 gcagttccga tttaagcgtc ttgtcgcaga aggcgaacag ttttttccag ccgtccaccg
     2401 cctgctcctg acgataggcc ggacgatcgt aatagaaaaa tccatggccg gcgccgtcgt
     2461 agcgatgaaa ctcgtaattc ttcccatgct ttttcagctc ggcttcgtgc aagttcacct
     2521 gttcgggcga gggagactta tcgtcgttac caaataagcc aatcatcggg catgaaagat
     2581 ccttggtgta atcaatcggc gcaacgggct gtttgggcgt gagctcctcc ttcgactgaa
     2641 ccacgcggcc gccccagcat tccaccagag catcgaagcc cttcaaacga caagcagcga
     2701 gaaacccgtg ccgcccgccg gagcag
//
LOCUS       DASULZ010000546         2349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_773751, whole genome shotgun sequence.
ACCESSION   DASULZ010000546 DASULZ010000000
VERSION     DASULZ010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773751"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /gene="dnaN"
                     /locus_tag="VFY96_11165"
     CDS             <1..1058
                     /gene="dnaN"
                     /locus_tag="VFY96_11165"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX5607068.1"
                     /translation="QSIVERRNTMPILGNVLIEAQKGSIRITATDLEVGVRGEVESDI
                     GKEGTVTVNAKKIYEIIREVPNQQVQVKRLENDWVELRSGKSVFKIVGMEAREFPQFP
                     KFDGKALSTTPAATIHEMIERTIFSVSTDETRYSLNGVFVEQGEGGKLRMVATDGHRL
                     AFEESSVGSLGLSKGVILPRKGLSELKKLLEAGDDGVVSLGFKDNMALVTKEKIELFM
                     RLIDGDFPDYTKVIPQGNPNVAKLEHDELLQALRRVSILSSERYKGVRMEFSDGRVSI
                     SANNPDLGEAVEEIEAEYKGKPLSIGFNARYLLDVLGVLTGEGEISIELKDELSPSII
                     RKVGVESYLYVLMPMRL"
     gene            complement(1074..1937)
                     /locus_tag="VFY96_11170"
     CDS             complement(1074..1937)
                     /locus_tag="VFY96_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HEX5607069.1"
                     /translation="MDKAQLIRQALSQSGQIVMPGVYDALSAKIAARSGFEVIFITGY
                     SLSATLLGEPDFGLLTQTEVIAAAQRICSVVDTPVIVDADTGYGNAINVMRTVRDLIA
                     AGAAGMFLEDQVWPKRCGHMKGKQVIPLDEQIKKLKAAIEAKQNRDFFIVARTDSRQA
                     LGLNAAIERGIAFKEAGADAVFIEAPESKEEMREISKQVRGPLVANMLERGVTPLMGP
                     NELKELGFDLIVWPLAPLYSVAKSLTEVYTTLRRDGSTLALLDRLMPFDEFNRVVGLD
                     EKYALDAKYRG"
     gene            complement(1987..>2349)
                     /locus_tag="VFY96_11175"
     CDS             complement(1987..>2349)
                     /locus_tag="VFY96_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607070.1"
                     /translation="MFGKGVFQEEENLLRNIIRSIDKRVDYSAKEGQGSRFTVHLKLR
                     ANEDDVVLNLEDLKAAKTDMVKRHQIRQKIKAKTDHLDKSRYADDILGLKPARLLKAS
                     PKPEPMMLRSGFGRGPRR"
BASE COUNT          572 a          586 c          619 g          572 t
ORIGIN      
        1 ctcaaagtat tgttgagcga cgcaacacca tgcctattct cggcaatgtt ctgatcgagg
       61 cgcaaaaagg atcgattcgt atcaccgcga ccgacctcga ggtgggagta cgaggcgaag
      121 tcgaaagcga catcggcaaa gaaggaacgg tcactgttaa cgccaaaaag atctatgaaa
      181 tcattcggga agttccaaat cagcaggtgc aggtaaagcg cttagaaaat gactgggtgg
      241 aactgcgcag cggaaagtcg gttttcaaaa ttgtcggcat ggaggcaagg gagtttccgc
      301 agtttccgaa atttgatggc aaggcgctgt ccaccacccc ggctgcaaca attcatgaaa
      361 tgatcgaacg gacgattttt tcggtttcca cggatgaaac ccgctacagc ttgaacggtg
      421 tttttgtcga gcaaggcgaa ggcggcaaac tcaggatggt cgcaaccgat ggccatcggt
      481 tggcttttga agaaagctcg gttggatctc ttggcctaag caaaggcgtt attttaccac
      541 gaaaaggatt atcggagctc aaaaaacttc tcgaagcagg cgatgacgga gtcgtgtctt
      601 tgggcttcaa ggacaacatg gcccttgtca cgaaggaaaa aatcgagctt ttcatgcggt
      661 tgatcgacgg cgattttccc gattacacca aggtgatccc acagggaaat cctaatgtcg
      721 ctaaactgga gcatgatgaa ctgttgcagg cgctgcgccg tgtatcgatt ctatccagcg
      781 agcgatacaa gggcgtcagg atggaattct cggatggcag agtttcgatt tcggcgaaca
      841 atcccgattt gggcgaagcg gtggaggaga tcgaggcgga atacaaaggc aaaccgctat
      901 ccatagggtt caacgcccgt tatttgctcg atgtcttggg tgtgcttacc ggggaagggg
      961 aaatcagcat tgagttgaag gacgaactca gcccaagcat catccgtaag gtaggggtag
     1021 agagttatct ttacgttctg atgccaatgc gactttaaaa aaaagaaggc cggctaaccg
     1081 cggtacttag cgtctaatgc atatttttca tctagaccaa caactcggtt aaactcatca
     1141 aagggcatta agcgatcgag gagtgccaga gtggagccat cgcgccggag tgtggtgtag
     1201 acctccgtca gcgatttggc gaccgaatag agcggcgcaa gcggccagac aatcagatcg
     1261 aaaccaagct cttttagctc gttaggtccc atgagcgggg tcacgccacg ctcgagcata
     1321 ttcgcaacca acggtccgcg aacctgcttt gaaatctctc tcatttcttc tttcgattcc
     1381 ggtgcttcga tgaaaaccgc atcagcccca gcttctttga atgcgattcc gcgttcaatg
     1441 gctgcattta accccaaagc ctggcgcgag tcggtgcgcg caacgataaa aaagtcgcga
     1501 ttctgttttg cttcaatcgc ggctttgagt ttctttattt gttcgtcgag tgggataacc
     1561 tgcttgcctt tcatgtggcc gcagcgtttt ggccatacct gatcttccag gaacataccg
     1621 gcggcaccgg cggcaataag atctcgaacc gttcgcataa cattgatggc atttccatag
     1681 ccggtgtccg cgtccacgat caccggcgtg tctaccaccg aacagattct ttgggcagcc
     1741 gcgattactt cagtctgagt caacaaaccg aaatcgggtt cacccaaaag cgttgccgag
     1801 aggctgtacc cggtaataaa tatcacctcg aaccctgaac gcgcggcgat ttttgcgctt
     1861 aaggcgtcgt aaacgccagg catgactatt tgcccggatt gactcagcgc ctgacgaatc
     1921 aattgtgcct tatccatagc tcggttctct gggatagctg ttatgatgct gttgctgaca
     1981 aacgctctag cgccttggcc cccggccaaa tccgctccgc agcatcattg gctccggttt
     2041 tggcgatgct tttagcagac gtgctggctt gaggccgagg atatcgtctg cataacgaga
     2101 tttgtcgagg tggtcagttt tggccttgat cttttggcga atctgatggc gctttaccat
     2161 atcggttttc gctgccttga gatcctccag attcagcacg acgtcatcct cgttcgcgcg
     2221 caacttgagg tgcaccgtga aacgcgagcc ttgcccctcc ttcgccgagt aatcgactcg
     2281 cttatcgata ctgcgaatga tgttcctgag taaattctct tcttcttgaa aaactccctt
     2341 accgaacat
//
LOCUS       DASULZ010000547         2428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_875893, whole genome shotgun sequence.
ACCESSION   DASULZ010000547 DASULZ010000000
VERSION     DASULZ010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875893"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VFY96_11180"
     CDS             <1..293
                     /locus_tag="VFY96_11180"
                     /inference="COORDINATES: protein motif:HMM:NF018032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HEX5607071.1"
                     /translation="RTLAEGLVNAMKDHIDQGQPLVLVFDADIAKLMGNIIERELIPG
                     AGIISIDGIDLKDFDFIDIGQELPDAKAVPVVIKSLIFRHTEHGRGHAHHAH"
     gene            448..633
                     /locus_tag="VFY96_11185"
     CDS             448..633
                     /locus_tag="VFY96_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607072.1"
                     /translation="MEEASGLQNFLEIVTKPDNIPIVAMLILVIFFTWLGLREAFKND
                     KLIEEGREDEIPNEMWK"
     gene            670..1455
                     /locus_tag="VFY96_11190"
     CDS             670..1455
                     /locus_tag="VFY96_11190"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5607073.1"
                     /translation="MGVATSYSAYGASLSRDDPRWQQARGDLADGKTSEAKAEFEKLL
                     AEDPSNADLHLFLAMALLRLRDPREAEAAARKAIALDPQHVDARTFLAWIELEVRGDV
                     DAAISEYEKVIELHPELPEAYSNLAVAQKRKGQLDRAVDNLNRALERKADFVTALTMR
                     GGILAEQNKWPEARRDFETALKLDPQDDGALYGLAQAMRESRDYAGAQQALRALVRRS
                     PNFVYWLEWGRIGLIRYWWVLLSVAVLVALRGRLMKGRIEANG"
     gene            1448..1993
                     /locus_tag="VFY96_11195"
     CDS             1448..1993
                     /locus_tag="VFY96_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607074.1"
                     /translation="MAEAKKRKGTALLAVMNESCSSCAGSPICEAHCPVDDCINLLYE
                     ELPGGGLKPYRVFVDNDKCIGCQMCYSDDLTKIQQHKETGEIFYEYANRFYDANRKPV
                     PPDAVPKRFQLQLIGTESEDRLDKKICPWDAIKMYEFDDGLVMSEYFYDKTKIKNVNG
                     QYVIDSEEKARLEEKQSELYE"
     gene            2015..>2428
                     /locus_tag="VFY96_11200"
     CDS             2015..>2428
                     /locus_tag="VFY96_11200"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367481.1"
                     /note="catalyzes the formation of oxaloacetate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HEX5607075.1"
                     /translation="MAEKIKIMETVLRDAHQCLVATRMRTEDMLPIAAELDEVGYWSA
                     ETWGGATFDATLRYLKEDPWERLRKLRAAMPKTRFQMLLRGQNVVGYRNYPDDVVQAF
                     VQRAAANGIDVFRIFDAMNDIRNMKTAVEAALKTGK"
BASE COUNT          617 a          584 c          695 g          532 t
ORIGIN      
        1 ttcggactct ggccgagggc ctggtgaacg ccatgaaaga tcatatcgat caaggtcagc
       61 cgttggtatt ggtattcgat gccgatatcg ccaagctgat gggcaatatt atcgaacggg
      121 agctgattcc cggcgccggc atcatctcga tcgatggcat cgatctgaag gattttgatt
      181 ttatcgatat cggccaggag ttgcccgatg ctaaagcggt tcccgtcgtc atcaaatcat
      241 tgattttccg ccataccgag catggtcggg gtcacgccca tcacgcgcat taaacgaaaa
      301 ctgaggatgg aagattaaga atgggagatg gtgagaacac gtaactgtcc aatatcttca
      361 tcctcgcttc tcgggcgaag tcacattgac tttacagagg tattttaata gtttgagata
      421 ttacatactt gcggatattt aattactatg gaagaagcca gcggactgca gaattttctg
      481 gaaatcgtta ccaagcccga caatatcccg attgtcgcga tgctgattct tgtgattttc
      541 ttcacctggt tggggctgcg cgaagcattc aaaaacgaca aattgatcga agaaggcaga
      601 gaagacgaaa tccccaacga aatgtggaaa taaatgttgt tgaggacatg gctggttttg
      661 gccgtcctca tgggggttgc tacttcttat tctgcatacg gtgcatccct atctcgtgac
      721 gatccgcgat ggcagcaggc gcgcggcgac ttggctgacg gtaaaaccag cgaagctaag
      781 gcagaatttg aaaagctgct ggcggaagat cccagcaatg ctgatctgca tttgtttctc
      841 gccatggcat tgcttcgatt aagggatccg cgcgaagccg aggctgcagc cagaaaagcg
      901 atcgctttgg acccgcaaca tgtagatgcc agaacgtttc tggcctggat tgaactggag
      961 gtgcgcggcg acgtggacgc ggcgatcagc gaatatgaaa aggttatcga gctgcatccc
     1021 gaactgccgg aggcatacag taatcttgcc gtggcgcaaa agcgcaaggg gcaactcgat
     1081 cgcgcagtcg acaacctgaa ccgggccctg gaacgaaaag ccgattttgt cactgcgctc
     1141 acgatgcgcg gcggaatttt agccgaacaa aacaaatggc ccgaagcgcg ccgcgatttc
     1201 gagacggcgc tgaagctcga tccgcaagac gacggcgccc tttacggcct ggcccaagcg
     1261 atgcgcgaat cgcgtgatta cgccggcgct caacaggcat tgcgcgcgct cgttcgacgg
     1321 tcgccaaact ttgtttactg gctcgagtgg ggcagaatag gattgattcg ttattggtgg
     1381 gtgctgttgt cggtggcggt ccttgtcgca cttcgagggc ggctcatgaa aggaaggatt
     1441 gaagcgaatg gctgaagcga aaaaacgcaa aggaacagcg ctcctggcgg tgatgaatga
     1501 aagctgctcg agttgcgccg gttcgcctat ctgcgaagcc cattgtccgg tggatgattg
     1561 catcaatttg ctctatgaag aactccccgg cggcggtttg aagccctacc gagtgttcgt
     1621 cgataacgac aagtgtatcg gttgtcagat gtgctatagc gatgatttga ccaagattca
     1681 gcagcacaag gaaaccggcg agatttttta cgagtatgcc aatcgcttct atgatgccaa
     1741 tcgcaagccc gtgccgccgg atgcggtgcc taagcgattc caactgcaat tgatcggcac
     1801 cgaatcggaa gaccgcctgg ataaaaagat ttgtccctgg gacgcgatca aaatgtatga
     1861 gttcgatgat ggactggtca tgtcggaata tttttacgac aaaacgaaaa tcaagaacgt
     1921 caacggccag tacgtaatcg acagcgaaga aaaagcgcgt ctggaagaaa agcaaagcga
     1981 gctctacgag tagctgagcc gtacggaatt aatgatggcg gaaaaaatta aaatcatgga
     2041 gacagtgctg cgtgacgcgc atcagtgtct ggtggcgacg cgcatgcgaa cggaggacat
     2101 gctgccaatt gccgccgaac tggacgaggt cggttactgg tcggcggaaa cctggggcgg
     2161 agcgaccttc gacgcgacgc tacgctactt gaaagaagat ccttgggaac ggttgcgcaa
     2221 attgcgagcc gccatgccga aaacccgctt tcaaatgctt ttgcgcggtc aaaacgtggt
     2281 cggctaccga aactaccccg atgatgtggt tcaggccttc gtccaacgcg cggccgccaa
     2341 tgggatcgat gtttttcgca ttttcgacgc gatgaacgac atccgcaaca tgaaaaccgc
     2401 cgtcgaggcg gcgctcaaga ccgggaaa
//
LOCUS       DASULZ010000548         2109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_773877, whole genome shotgun sequence.
ACCESSION   DASULZ010000548 DASULZ010000000
VERSION     DASULZ010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773877"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VFY96_11205"
     CDS             complement(<1..242)
                     /locus_tag="VFY96_11205"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5607076.1"
                     /translation="METRNYTAEQEAEMLRREESHHFLDFKRAIITPGKLQETVTALA
                     NADGGEIIIGLDDDIRRSPIDRFHGFPKVITYPQHLP"
     gene            complement(301..528)
                     /locus_tag="VFY96_11210"
     CDS             complement(301..528)
                     /locus_tag="VFY96_11210"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX5607077.1"
                     /translation="MKMNLRELHLVELRTMPQGHPDYRFICQEMWRKIQEIHPALAEA
                     EKFIDWRKYRLGRLQSEMRTEFKKSALKNKA"
     gene            complement(540..821)
                     /locus_tag="VFY96_11215"
     CDS             complement(540..821)
                     /locus_tag="VFY96_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002767514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEX5607078.1"
                     /translation="MRMKNPPHPGRIVRQECIEPLGLTVTETAKRLGVTRQALNNLVN
                     GKGGISAEMAVRLSKAFGSSPEVWLGMQMEYDLAQVEKEARNIKVKRVA"
     gene            complement(835..1113)
                     /locus_tag="VFY96_11220"
     CDS             complement(835..1113)
                     /locus_tag="VFY96_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007244538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX5607079.1"
                     /translation="MIQSFKHKRLRQLFEKGTAKGIRADHVAKVENILAVLNRARKPS
                     DMDLPGFGLHPLKGDLKNFWSVTVRANWRIIFRFEDGEVHDVDLLDYH"
     gene            complement(1529..>2109)
                     /locus_tag="VFY96_11225"
     CDS             complement(1529..>2109)
                     /locus_tag="VFY96_11225"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX5607080.1"
                     /translation="MSADQRRQILAEHFAKRRHRRDKLSRAFENVYYSFDILGNLGLY
                     RDLQRHRILTQERQDFTTVHGYDTPPEIKEAGFKNEFDECMKRAADLYEQIYADLPSE
                     AQYVVPFAYKIRWYMKMNLREALHMCELRTMPQGHPDYRFICQEMWRKIQEVHSTLAE
                     CGKFIDSKKYRLGRLQSEMRTEFKKSGMESKK"
BASE COUNT          427 a          596 c          547 g          539 t
ORIGIN      
        1 ggcagatgtt ggggatatgt aatgactttc ggaaaaccat gaaagcggtc gattggggat
       61 cgacggatgt cgtcatcgag accgattatt atttcaccac cgtcagcatt cgctagagcg
      121 gtgacagtct cctgaagctt gccgggcgtg atgatcgcac gcttgaagtc caaaaagtgg
      181 tgcgactctt cgcgacgaag catctccgct tcctgttcgg cggtataatt tctggtctcc
      241 atacgatatg tattctaact actgacggca ccgacaatcg tcgcgtatcc ggaatatact
      301 ctacgcttta ttcttcagtg ccgacttctt aaactccgtc cgcatttcgg attgtagccg
      361 gcccaaccgg tacttccgcc agtcgatgaa tttctcggct tcggccagcg caggatgaat
      421 ttcctggatc ttgcgccaca tctcctggca aatgaagcgg tagtcgggat gaccttgcgg
      481 catggtgcgc agctcgacca ggtgcagctc gcgcaggttc attttcatgt accagcaact
      541 cacgcaaccc gtttcacctt gatgttgcgg gcctccttct cgacttgcgc gagatcgtat
      601 tccatctgca tgccgagcca cacttccggg cttgacccga acgctttgga aaggcgcacc
      661 gccatctcgg ccgagatacc gcccttgccg tttaccaaat tgtttagtgc ctggcgcgtc
      721 acaccgaggc gctttgccgt ttcggtcacg gtcaatccca acggctcgat acactcttgg
      781 cggacaatgc gtccaggatg gggcgggttc ttcatgcgca tgacttaatc tcctttagtg
      841 ataatcaagc aaatccacgt cgtgcacttc gccgtcttcg aaacgaaaaa tgatccgcca
      901 gttggctctc actgttacgc tccagaaatt tttgagatca ccctttaatg ggtgcaggcc
      961 gaagccgggc aagtccatat cggatggctt tcgagcgcga ttcagaacgg caagaatatt
     1021 ttcgactttg gcgacatgat cggcgcggat ccctttagcc gtgccctttt caaatagctg
     1081 tctcaagcgt ttgtgcttga agctttgaat cacgccgcca atttaacacc agcggtgaca
     1141 acaagcaatt gtcactaggc agcgaagtgt acggattccg ttcatctcgt tcggaacctc
     1201 gcatgtttgc tcaattgcgc aacagttgcc gcagaaccct ctgacacacg ggaggaaata
     1261 cgagcctatc cgcgatcgcg agctagtttt ctggccttca ttttcacgtc cgcccacaga
     1321 atcggccgga caaaagcttg tttaatctca acagcacgtc gttcaacttc cgccgcccag
     1381 cagcgtcaac ctcgcgggct tccggttcca acctggctga aggttttccg acggtttctt
     1441 tcgcttttcg agtcatgaat caatccactc tcattgaccc tgccagaggg ggcccagcgc
     1501 acgaacgttt ttaactttat gattggggct attttttgct ttccatcccc gatttcttaa
     1561 actcagtgcg catttctgac tgcaaccgtc ccaaccgata cttcttcgaa tcgataaact
     1621 tgccgcactc cgccagcgtc gaatgtactt cctggatctt gcgccacatc tcctggcaga
     1681 tgaagcggta atcgggatgg ccttgcggca tcgtacggag ctcgcacatg tgcagcgctt
     1741 cgcgcaggtt cattttcatg taccagcgga tcttgtaggc gaacgggacg acgtattgcg
     1801 cttcgctcgg cagatcggcg taaatctgct catatagatc cgccgcgcgt ttcatgcatt
     1861 cgtcgaactc gtttttgaag cctgcttctt tgatctccgg cggcgtgtcg tagccgtgca
     1921 cggtggtgaa gtcctggcgc tcctgggtga ggatccggtg gcgctgcaag tcgcggtaga
     1981 ggccgagatt accgaggatg tcgaagctgt aatagacgtt ctcgaaggcg cggctgagtt
     2041 tgtcgcgccg gtggcgccgc ttggcgaaat gctcggcgag gatctgccgc cgctgatcgg
     2101 cgctcatcg
//
LOCUS       DASULZ010000549         2283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_266565, whole genome shotgun sequence.
ACCESSION   DASULZ010000549 DASULZ010000000
VERSION     DASULZ010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266565"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="VFY96_11230"
     CDS             <1..779
                     /locus_tag="VFY96_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607081.1"
                     /translation="RIWADENGRDCIGAVAILDEQIFTSRDVQKADARPGGYVATGGH
                     GGIIGRIGHPGAPQFSFKPVKRHTYDSAVNLTRLPREVQGSSIEASAIQTVSVAIKRE
                     DGNLLASAIPKVTIVKHARYLPDDTSGNAGAEVDILARIEKNLRDAPLAGFVAEGSAP
                     FGAMSNSVDAALKRAVFSGMPVVKVGRGNAGGFVDPTRDPLAIAGSNLTATKARLLLM
                     ACLMKFGCLPPGADPAKPSAAEIEATKAKLKEYQTIFDTH"
     gene            804..1877
                     /locus_tag="VFY96_11235"
     CDS             804..1877
                     /locus_tag="VFY96_11235"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEX5607082.1"
                     /translation="MSGKHGHKPFEGHMHDYLAHIKAVEEDLEYYRAKRFEPRIIYLL
                     RRGVVTFYDLLKVCATLKKKDHFFKSRKPQGKNIEARVRYLEEWLDEYVKGIALVSAS
                     AVEGMDMVTEDNRKERGDYDDFFKIKKKEHHGALEERMSNLEQDLTDYHELLEVFVQA
                     LIKRGWATRTDLEERWKQLHEERPWAGGVIVAKAWNDPEFKQALLTTGREALREMGVH
                     QGKVGKVVIVENTDKVHNVIVCTLCSCYPYDILGDTPWWYKHESYRSDIVQNPRQCIK
                     EMFRLEIPPEKEVRVWDSTSDVRYFVLPERPAGTESMSEEELAKLVTVDSLIGAGYAL
                     EPAQLSEREAAHVEPEIPRVRPD"
     gene            1884..2165
                     /locus_tag="VFY96_11240"
     CDS             1884..2165
                     /locus_tag="VFY96_11240"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HEX5607083.1"
                     /translation="MTEMSSKYKVGDAVVVAEENPRGNPRTPRYIRGKRGVIGAVHGV
                     LENIRDHRGVHQPLYTVKFDLAELSSCHDQDTIWVDVHEEWLSEMRRDS"
BASE COUNT          566 a          586 c          672 g          459 t
ORIGIN      
        1 ccagaatctg ggccgatgag aacgggcgcg attgcatcgg cgcagtggcg attctcgacg
       61 agcagatctt tacatcgcgg gacgtgcaaa aagcggatgc gcggcccggc ggttacgtcg
      121 ctactggcgg acacggcgga atcataggcc gaatcggtca ccccggtgcg ccgcaatttt
      181 cattcaagcc ggtgaagcgc catacgtacg attcggcggt gaacttgact cggctgccga
      241 gagaggttca aggctcaagc attgaagctt cagcgatcca aacggtcagc gtcgcaatca
      301 aacgtgagga tggtaatctg cttgcgagcg cgattcccaa agtgacgatt gtgaagcatg
      361 cgcgttactt acctgatgat acgtcaggga acgcgggcgc tgaagtcgat atactcgcac
      421 gcattgagaa aaatttgcgc gatgctccgt tagcgggttt tgtcgccgaa ggatcggcgc
      481 cgtttggcgc gatgagcaac tccgtggacg cggcactcaa gcgcgcggtg ttcagcggca
      541 tgccggtggt gaaagtcggg cgcggtaatg ccggcggttt tgtggatcca acccgtgatc
      601 cgctggcgat cgccggaagc aatttgaccg ccaccaaagc gcggcttctg ttgatggctt
      661 gtctgatgaa attcggttgt ttaccgccgg gggcggatcc agcgaagccg agcgcggcgg
      721 aaattgaggc gaccaaagcc aagctgaaag aatatcagac gatattcgat actcattgag
      781 agcgtatccg gaggagtttt aaaatgtcgg gtaaacatgg gcacaagccg ttcgaagggc
      841 acatgcacga ctacctggcc cacatcaaag ccgtcgaaga ggatctggaa tattaccggg
      901 ccaagcgctt cgaacctcga attatctatt tactgcggcg cggtgtcgtc acgttctatg
      961 acctgctcaa ggtttgcgcg acgctgaaaa agaaagatca tttttttaaa agtcgtaaac
     1021 ctcaaggaaa aaatatcgaa gcacgcgtgc gctacctgga agaatggctc gatgaatacg
     1081 tgaaaggcat cgctctcgtt tccgccagcg ctgtcgaagg catggacatg gtgacggagg
     1141 acaaccgcaa ggagcgcggc gattacgacg actttttcaa aatcaagaaa aaggaacacc
     1201 atggcgcact cgaagagcgc atgagcaatc tcgagcagga tctaaccgat tatcatgagt
     1261 tgctggaagt tttcgtccag gcattaatca aacgcggctg ggcaacccga acggatctcg
     1321 aggagcgctg gaaacaactg catgaagaac gcccttgggc cggcggcgta atcgttgcga
     1381 aggcttggaa cgatccggag ttcaagcagg ctttattgac caccggccgc gaagcgctaa
     1441 gagaaatggg cgtacatcag ggcaaagtcg gcaaagtcgt tatcgtcgag aacaccgaca
     1501 aagttcataa cgtgatcgtc tgcacgctct gctcctgcta tccgtacgac attctcggcg
     1561 acacgccctg gtggtataag cacgagagct atcgaagcga tatcgttcaa aacccacggc
     1621 agtgcatcaa ggaaatgttc cgcttggaaa ttccgccgga aaaagaggtt cgtgtctggg
     1681 atagtacgtc tgacgtgcgc tatttcgttt tgcccgagcg ccccgccggc accgagagca
     1741 tgtccgaaga agaattggcc aagcttgtta cggtcgatag cttgatcggc gccggatatg
     1801 cgctagaacc ggcgcagttg agcgagcggg aagcggctca tgtcgaaccg gaaatacccc
     1861 gcgtgcgccc ggactgagaa gccatgaccg aaatgagcag caaatacaaa gttggcgatg
     1921 cggtcgtagt cgccgaggaa aacccgaggg gcaatccgcg cacgccgcgc tatatccgcg
     1981 gcaagcgcgg cgtcatcggc gccgttcacg gcgtgctcga aaacatccgc gatcatcgcg
     2041 gcgtgcatca gccgctttac acagtcaagt ttgatctagc tgaactctcc agttgccacg
     2101 atcaagacac gatttgggtc gatgtgcatg aggaatggct gagtgaaatg agacgggata
     2161 gctaaactgc agcctcaaaa agatttatta acgccagatc tgctcggcgg tttccacgat
     2221 cgtatcgagt tttctccaca tttcgtcttc gctcagcact ttggaatcga tgccgccgaa
     2281 gcc
//
LOCUS       DASULZ010000550         9343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_367835, whole genome shotgun sequence.
ACCESSION   DASULZ010000550 DASULZ010000000
VERSION     DASULZ010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9343
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367835"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /gene="sucB"
                     /locus_tag="VFY96_11245"
     CDS             <1..975
                     /gene="sucB"
                     /locus_tag="VFY96_11245"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR01347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006554 - lysine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEX5607084.1"
                     /translation="ATPVTEPAKDGTIEKHQDSPQPDEVDEAVAEAGAETVPEAEALP
                     KFESRAAPPEKVPIHLPLDQRVADDADRQPQSPGAPEKIRTPSQLPDGNRERIVAMSH
                     LRRRIAERLVGAQHNAALLTTFNEVDMSSVIELRKRYRDLFQERYQAKLGFMSFFVKA
                     SIAALRTIPEVNAEIRGTDIIFRNFFDIGVAVGGGKGLVVPVLRNAERLTFAEIEHAI
                     ADFARRAEQSKILPAELQGGTFTISNGGIYGSLLSTPIVNPPQSGILGLHAIQDRPVA
                     RDSAVVIRPMMYLALTYDHRLIDGREAVQFLKHIKETIEDPARLLLDI"
     gene            complement(1008..1424)
                     /locus_tag="VFY96_11250"
     CDS             complement(1008..1424)
                     /locus_tag="VFY96_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607085.1"
                     /translation="MIQAKRGFQVIALSVAMAAAGCESVALLPRADVDQRDRAGTSDA
                     TIGRTERDRSRSSAEITGTVQRVDDSRREIMVRTRNEVVVVKYDPRTVVYDRDRELRV
                     SDLRNGDQVSIQLGRDVGYDRYADVIRMSDRRSQTY"
     gene            1815..1979
                     /locus_tag="VFY96_11255"
     CDS             1815..1979
                     /locus_tag="VFY96_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005984980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEX5607086.1"
                     /translation="MLNWSITFLIIGLIAAVLGFTGVAGAATQIAWVLFVVFLVLFLV
                     SLVMGRGRVG"
     gene            2203..3102
                     /locus_tag="VFY96_11260"
     CDS             2203..3102
                     /locus_tag="VFY96_11260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX5607087.1"
                     /translation="MPRKSKTKRKTAESKSGSSRAIWKGSIDFGLVNIPVRLYTAENS
                     HSLSFDLLDKRDFSRIRYRRVNEKTGKEVPWDEIVKGYEYKKGEYVALSDGDFARANV
                     EATQTISITDFVDASAVSPLYYDKPYYLEPLKNGQRAYALLREVLHNTGKVGIAKVVI
                     RSREHLAMVLAEGPALILELLRFPDELRDASRLDLPKAGSKGTAISAQEIKMAERLVE
                     SMIGKWQPEKYRDEYRDDLMKIVDEKVESGKTKMVDTAMPAAPREQRGKVIDIMHLLR
                     ESVEQASKRGPRKDERTSRRKAG"
     gene            3472..4569
                     /locus_tag="VFY96_11265"
     CDS             3472..4569
                     /locus_tag="VFY96_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009282534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5607088.1"
                     /translation="MNGSSRQSHEAGRGNLAKLGVLAALAAGAVYAMRERRWIDFKGK
                     NVLISGASRGLGLELARIFAAEGAGLLLLARDAERLRQTAGELERYGSRIFTFTCDIT
                     DPVQLRQAVGAVLQTVSHVDVLINNAGMIQVGPMENMALSDYEHAMAVHFWGPLQLIN
                     AIAPHMKARRQGRIVNITSIGGKVAVPHLLPYAASKFALVGLSEGLRAELLKDGVYVT
                     TVCPGLMRTGSHLQAFFKGQHKKEYALFAIANASPLLSTSSQAAARAIVEACRSGKAE
                     ITITPQARLLRIVHGLCPNFVTDTLAIVNRALPKPRVEAGGNVAKEGWASRSMLAPSL
                     LTRGSDAAAARNNELPSGNGSIQLSAARGTK"
     gene            4676..5128
                     /locus_tag="VFY96_11270"
     CDS             4676..5128
                     /locus_tag="VFY96_11270"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5607089.1"
                     /translation="MDAIEFLTKDHDALKAVLSAANTYDEMKAAFGEISRILETHTYL
                     EERFFYPGFENHEHLRQLIQEAREQHDLVKQVLEGMKHQNGQEFERNFQTLRAEVQLH
                     MTSEEAEIFPQAESSTDHATLAHLGSELETAKMAFESGSESSTAASPR"
     gene            complement(5354..5962)
                     /locus_tag="VFY96_11275"
     CDS             complement(5354..5962)
                     /locus_tag="VFY96_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607090.1"
                     /translation="MTALTLVWTVRPRLRAITAAERLISFGDALIIFNGAITILVSAI
                     LFCHCFVHLLSQSDTSRVGEIVFAGKTSDCLPVLLSFGGFQLIAKPIQSSLIAVLTYF
                     WKHFIFLFRHVVFHVFHQLFQRGAKLFAFGIKSLNLFQQCFDFLMLFQGFFDHVFQLI
                     NLLHLGIEDGFLDVRMDVQLGVDLVEYSLPLLGVFRIIDLIE"
     gene            6060..6629
                     /locus_tag="VFY96_11280"
     CDS             6060..6629
                     /locus_tag="VFY96_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEX5607091.1"
                     /translation="MAGKNTSAFGIYPNRAALEMGIQALKDANFRHEDISVLLPENVG
                     TKDFAHEKDTKAPEGATTGAGTGAVIGGVLGWLAGIGALAIPGLGPFIAAGPIMAALA
                     GLGAGGAAGGIIGGLVGLGIPEYEAKRYEGRIKEGGILVSVHCDNSEWTRKAKDILER
                     TGAEDVSSAGEASADFDEHDKPRPRTDVV"
     gene            complement(6681..8102)
                     /locus_tag="VFY96_11285"
     CDS             complement(6681..8102)
                     /locus_tag="VFY96_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5607092.1"
                     /translation="MNRSKGYDAVVIGAGPNGLAAAITVAQAGKSVVVFEANDSVGGG
                     VRSAELTLPGFSHDVCSSVYPLALGSPFFRTLPLHKHGLEWIQPAAPLAHPLDDGSAV
                     LLHRSVDETMKGLGHDAVSYQRVFRWLAHCWDGLDADILAPPHVPRHSLQFLRFGYSA
                     IQPARRFAMGRFAGLRGRALFAGLAAHSMLPLDSWGSAAFGLVLGAAAHAFGWPVVRG
                     GAQRLADALGSYLISLGGEIVLGRSVESLEELPYSRAVLCDLTPRQLATIAGTRLAPG
                     YYRKLARFRYGMGAYKIDWALSAPVPWRAAECALAATIHLGGTVEEIAESEKAAWNGQ
                     HAPRPFVLVVQPSLFDDLRAPGGKHTLWGYCHVPNGTTKNMTEAIESQIERFAPGFRD
                     CVLARAVAPPAELERRNANLVGGDINGGAPDIRQLFLRPNRKLYSTSEKGLYLCSAST
                     PPGGGVHGMCGHFAARRALREIS"
     gene            complement(8371..8724)
                     /locus_tag="VFY96_11290"
     CDS             complement(8371..8724)
                     /locus_tag="VFY96_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607093.1"
                     /translation="MEESLETIRRDIENTRFALGEKLGRLEHKIEITTKQTLNPAYYV
                     RTRPWPTLGAFAVLGWMVGRALKPKRNRTRHAAPPTLAGSVVRSVVSGAATALGGAAG
                     DLVRDIVKDRRRRVP"
     gene            complement(8739..9164)
                     /locus_tag="VFY96_11295"
     CDS             complement(8739..9164)
                     /locus_tag="VFY96_11295"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX5607094.1"
                     /translation="MDPPYQTRGGNDRAESVIELMRGIVQDARTLSTKELTAAKLEIT
                     QEIKKIVSSSILLGISVFILAVAFVLLSIVIALALAQYIPLPTWAGFAIVGGVYLVVG
                     LIVLFVGRRKLQTAKPIPEHTLRGAKADAQYIREKATGH"
BASE COUNT         2206 a         2566 c         2649 g         1922 t
ORIGIN      
        1 gcgactcccg tgactgaacc ggccaaagac ggcacgattg aaaagcacca ggatagcccg
       61 caaccggacg aagtggatga ggcggtcgcc gaggcaggag ctgaaactgt tccagaagcc
      121 gaagcgttgc cgaagttcga atcgcgcgct gcgccgcccg agaaagtgcc catccacctt
      181 ccgctagatc agagagtagc cgatgatgcc gaccgccagc cccaatctcc tggtgccccg
      241 gaaaaaatcc gtacgccatc gcaattgcca gatggaaaca gagaacggat cgtcgcgatg
      301 agccatttgc ggcggcggat tgccgagcga ttggttggcg cgcagcacaa cgccgctctg
      361 ctgacgacct tcaatgaagt ggatatgtcg tcggtgatcg agctacgcaa acgttatcgc
      421 gatctgtttc aggaacgcta tcaagccaag ctcggcttta tgtcgttttt cgtgaaggca
      481 agcatcgccg cgctcaggac gataccggag gtcaatgcgg aaattcgcgg cacggatatc
      541 atcttccgga acttttttga tattggcgtc gccgtcggtg gcgggaaggg tctagtggtt
      601 cccgtgctgc gcaacgccga gcgtctaaca ttcgccgaga tcgagcatgc catcgccgat
      661 ttcgcgcggc gcgccgaaca gagcaaaatt cttcccgcgg aactgcaggg cggtacgttt
      721 acgattagca acggcggcat ctatggatcc ttgctgtcca caccgattgt caatccgccg
      781 caaagcggga ttctgggatt gcacgcgatc caggaccgac ctgtggctcg cgacagcgct
      841 gtggtcattc ggccgatgat gtacctcgcc ctcacctacg accaccgcct gatcgacggc
      901 cgtgaagcgg tacagtttct aaaacacatc aaggaaacga ttgaagaccc agcacgattg
      961 ctgctcgata tttagcggcg tcgaccgctc cgaagtcgcc gccgcttcta ataggtttga
     1021 gagcgccgat cgctcattcg gataacgtcc gcataacgat cgtatcccac gtctctgccg
     1081 agctgaatcg agacttgatc accatttcgc aaatccgaaa ctcgcagctc tcggtcgcgg
     1141 tcgtatacga ccgtgcgcgg gtcatatttt acgacgacga cttcattacg ggttcgcacc
     1201 atgatttcgc gtcttgagtc gtcgactcgc tgcaccgtcc cggtaatttc cgctgagctt
     1261 cggcttcgat cccgttcggt ccgtccgatg gttgcgtccg aagtaccggc gcgatcacgt
     1321 tgatccacat cggctcgggg cagcagcgcc acgctttcac aaccggcggc ggccatcgcc
     1381 acggagagag ctataacttg aaatccacgt ttcgcctgaa tcatgtaaat cacctccgct
     1441 gtaaattccg cccgaaaagg tcgcaagcaa ggtgccagtt gagctagtag agagaccacg
     1501 gagttcctac ctggcaaagc ggcgcgatcg aaattctgat ccgaattctt tcgtggactg
     1561 gaagaaatgc gctgacaacg tgtgggcgga attcccccaa agtggaatcg ttttcgacgc
     1621 ggggcattaa gggaagtcga ttcattagaa acaacagcgc cggagccacg actgaaatca
     1681 ctaggcgatc aaacgggtta gcgacgcgag tgcatcgcca ttgaagccca gcggcacgtt
     1741 ggttgcgatg ttttctgttc ggccggccat gagctgctgc ttgtaaatca ttataaataa
     1801 aaggaggaga gacgatgctt aactggtcga tcacattttt aatcattgga ctcattgccg
     1861 cggtgctcgg ttttacgggc gtggccggag ccgcgactca gatcgcttgg gtcttgttcg
     1921 tcgtttttct ggtgctcttc ttggtcagct tagtcatggg ccggggcagg gtcggctaga
     1981 gccgattcgc taccatgttt aagccaaccc gcttggcgag ctagcgtacg tatctcactc
     2041 ttatgccgcg taaaccgcgc acaaaaggga aaaaaagcgc cggcgcgccg cgggatccgg
     2101 aaacccgcgg tcgcgccgcg aaagttcgga cacgcagcgg cgagaaatgc accacgagaa
     2161 ataaaaaacc cgtgtcttga agcttccaac tgcaaggtgt gaatgccgcg taagtccaag
     2221 acaaaacgta aaaccgcaga atcaaagtca ggttcctcca gagcgatttg gaagggcagc
     2281 atcgactttg gtttggtaaa cattccggtt cgcctctaca ctgcggaaaa ttctcatagc
     2341 ttgagttttg atctgttgga taaacgcgat ttttcgcgca tccgctaccg ccgggtaaac
     2401 gaaaaaaccg gcaaggaagt gccgtgggac gaaatcgtta aggggtacga atacaaaaag
     2461 ggcgagtatg tcgcgctttc tgacggagat ttcgcccgcg ccaatgtcga agccacgcag
     2521 acgatatcga tcaccgattt tgtcgacgcc agcgcggtca gcccattata ttacgataag
     2581 ccatactact tggaaccgct gaagaacggg caacgagcct atgcgttgct acgtgaagtt
     2641 ctccataaca ccgggaaggt ggggattgcg aaagtggtga ttaggagccg cgagcacctg
     2701 gccatggtgc tcgcagaagg gccggctctg attttggagt tactgcgttt tcccgacgag
     2761 ttgcgtgacg cttcgcgtct cgatttgcca aaagccggtt cgaaaggaac agccatatcg
     2821 gcgcaagaaa ttaaaatggc tgaacgtcta gtcgaatcga tgattgggaa atggcagccc
     2881 gagaagtacc gtgacgaata ccgcgacgac ctaatgaaaa tcgtcgacga gaaagtcgaa
     2941 tccggcaaga ccaagatggt cgacaccgct atgcccgcgg ctccgcgcga gcagcgcggc
     3001 aaggtcattg acatcatgca cctgctccgc gagagcgttg aacaggcgag caaacgcggg
     3061 ccacggaaag atgaaagaac cagtcgccgc aaggccggat aactctcatc cctcacgacg
     3121 aacttcccct tcaaatgcat ctttcgtccc aactctagca ccagggatgt tgttactgtc
     3181 gcatatgtgc gagccacgaa tgaacgatag gatcaatttg cgcacggcgc gaaggtgact
     3241 ggtcctgcta cacgatcctc cacgtggcat attgattgcg ttttatggcc gcacggggac
     3301 atcattacct taaaagttca gcgatcagct acagggcgag aggcgggcgg tgctgagaga
     3361 acttccccat aacgaagaag gctttgagtc ggactatgag ctacctgatg cgccccccac
     3421 caccgccgcg tcgaggtccc tagagacgcc aacatctcat ggagttaagg gatgaacggg
     3481 agttcacgcc aaagccacga agctggaagg ggaaacctcg ctaagctcgg tgtgctcgcc
     3541 gcgcttgctg caggtgcagt ctacgcgatg cgtgaacggc gctggatcga cttcaaaggc
     3601 aagaatgttc ttatttccgg tgcgtcgcgc ggccttggcc tggagcttgc ccggattttt
     3661 gcggcagaag gcgccgggct cctgctgctc gcccgtgatg cggagaggct cagacaaacc
     3721 gcaggcgagc ttgaacggta cgggagccga atttttactt tcacctgtga catcacggat
     3781 cccgtccagt tgcgtcaagc cgtgggagcg gttttacaaa ccgtgagcca tgtcgacgtg
     3841 ctgatcaata atgccggaat gattcaggtc ggcccgatgg agaacatggc cctcagcgat
     3901 tatgagcatg ccatggctgt tcatttttgg ggaccgctgc aattgatcaa cgccatagca
     3961 ccccacatga aggcgcgccg gcaggggcgg attgtgaata tcacgtcgat cggcggcaaa
     4021 gttgctgtac ctcacctgct gccctacgcc gcgagcaagt ttgcgctagt cggtctctcg
     4081 gaaggactcc gtgcagagct tcttaaggac ggtgtttacg tcaccacggt ttgccctggc
     4141 ttgatgcgca ctggctctca tcttcaggcg ttcttcaaag gacagcataa gaaagaatac
     4201 gcgctgtttg cgattgctaa tgcctcaccg ttgctctcga ccagctccca agccgccgcg
     4261 cgtgctatcg tcgaggcgtg ccgttccggt aaagcggaga tcacgatcac gccgcaagct
     4321 cgcctacttc gcatcgtgca tggtctgtgc ccgaattttg tcaccgacac actggcgatc
     4381 gttaaccgcg cactgccgaa gccgagagtg gaagcaggcg gaaacgtcgc gaaagagggc
     4441 tgggccagcc gttcaatgct cgcgccctct ttgctaaccc gaggctctga tgctgcggcc
     4501 gcgcgcaata acgaactccc atccggcaac ggcagtattc aactcagtgc cgcgcggggc
     4561 acaaagtgaa acaattcagc aatgcacgac ttcaatccga gccgtgccaa cgtcgtcagc
     4621 aacaaccaac gcgcgcccta gtcgcccgtc gcagccgcga ttcatagagg tttgcatgga
     4681 tgcgattgaa tttttgacca aggatcatga cgccctgaaa gcggtgctgt ccgcggccaa
     4741 tacctatgat gagatgaaag ctgcctttgg ggaaattagc cggattctcg agacccatac
     4801 ttatctggag gaacgtttct tttatcctgg cttcgagaac catgagcatc ttcgtcagct
     4861 gattcaagag gcccgcgagc agcatgatct cgtcaaacaa gttcttgagg gtatgaagca
     4921 tcagaatggt caggagttcg aaagaaattt tcaaaccctc agggccgaag tgcagctgca
     4981 catgacttcg gaagaagccg agatttttcc ccaggccgag agttcgacag atcacgcaac
     5041 tctggcacac ctcggcagtg agctggaaac cgcaaagatg gcgttcgaga gcggctcgga
     5101 aagctccact gcagcttcac cgcggtgatt gcgcaacgga aaccgtgtcg gcttaatcga
     5161 acatctcgca ccgtttcttc gggtgcttcg ctgacacaaa ataattgtcg atggaaactc
     5221 tcttgatcga ctggggtttt tttcttcacg gttatggcat ctgccttgct cgcctaaaca
     5281 ttaagatttt tttggaggtg aattcatgga cgcattgcaa ttacttaagc aagaccatca
     5341 atcggtcaag gatctattcg atcagatcga tgatccggaa gacgccaagc agcggaagag
     5401 aatattcgac cagatcgaca cccaattgaa catccatgcg cacatcgagg aaaccgtctt
     5461 ctatcccgag atgcagaaga ttgatgagct gaaagacatg gtcgaagaag ccttggaaga
     5521 gcatcaggaa gtcaaaacac tgctggaaga gattgaggga cttgatcccg aaggcgaaca
     5581 gtttagcgcc tcgctggaag agctgatgga aaacgtggaa caccacgtgg cggaagagga
     5641 agatgaaatg tttccaaaag tacgtgagca ctgcgatcag gctactttgg atcggcttgg
     5701 cgatcagctg gaatccgcca aaggaaagca acaccggcag gcagtctgat gttttgccgg
     5761 caaatacaat ttccccaact cggctggtat cgctctgaga aaggaggtga acaaaacaat
     5821 ggcaaaacag gattgcgctc acgaggattg tgattgcacc gttgaagatg atcagggcgt
     5881 caccaaagga gatcaaacgt tctgcagcag ttattgcgcg caagcggggt cgaacagtcc
     5941 agacgagtgt cagtgcggtc atgaggactg ttcttgatca cggcgatgtt caggggcgcg
     6001 tgtgctgcca agcataaatt gcgcgagcat ggctcattaa ccacgaaagg aataaattta
     6061 tggcaggcaa aaatacttcg gcatttggca tttatcctaa ccgagcggca ttggagatgg
     6121 gcattcaggc attgaaagac gccaattttc gtcacgagga catctcagtg ctcttacccg
     6181 aaaacgtagg tactaaggat ttcgcgcatg aaaaagacac caaggcgccc gagggcgcaa
     6241 ctacaggagc gggtactggg gcagtgatcg gcggtgttct cggatggttg gctgggatcg
     6301 gcgcgcttgc aattccagga ttaggaccat ttatcgctgc cgggccgatc atggccgccc
     6361 tcgcaggact gggcgccggc ggtgcggctg gcggaattat cggcgggttg gtgggtctgg
     6421 gcattccgga atatgaggcg aaacgttacg aaggccggat taaggagggc ggcattttag
     6481 tctccgtcca ctgtgataac tcggaatgga cgcgcaaagc caaggatatt ctggagcgaa
     6541 cgggcgctga ggatgtctcg tctgctggag aagcgagtgc tgacttcgac gagcacgata
     6601 aaccccgacc tcgcaccgat gttgtttgat caacgattat ggacgaacgc cgcggttatg
     6661 tcgcgcggaa gattgggcag ttaggatatc tcgcgcaggg cccgtcgggc ggcaaaatgg
     6721 ccgcacattc cgtgcacacc gccgccgggc ggcgttgaag ccgagcatag gtaaaggccc
     6781 ttttctgaag tggagtaaag cttgcggttc ggccgcaaaa aaagttgccg aatgtcgggg
     6841 gcgccgccgt tgatgtcgcc tccgacaagg ttagcattgc gccgctcgag ctcggcgggc
     6901 ggcgcaacag cacgggcgag aacgcaatcg cgaaaccccg gcgcaaaccg ttcgatttgg
     6961 ctttctatgg cttccgtcat attcttagtg gtgccattcg gaacgtgaca gtaaccccaa
     7021 agcgtgtgct tcccgcctgg agcgcgcaga tcatcaaaca aactgggttg gacaaccagc
     7081 acgaacggcc ggggcgcgtg ctgcccgttc caagctgcct tttcggactc cgcaatctcc
     7141 tcgacggttc cgcccagatg aatcgtggcg gcaagggcgc attcggccgc tctccagggt
     7201 accggagccg agagtgccca gtcgattttg tatgcgccca ttccgtagcg aaagcgcgct
     7261 aatttgcggt aatacccagg ggccaagcgc gtgccggcta tagttgctaa ctgccgtggc
     7321 gtcagatcgc aaagtacagc gcgcgagtac ggtaactctt cgaggctctc tacggaacgt
     7381 cctagcacga tctctccacc aagtgagatt aagtaggaac ccaaggcgtc ggcgaggcgc
     7441 tgcgcgccac cacgcaccac tggccagcca aaagcgtgcg cggcggctcc tagcaccaag
     7501 ccgaacgccg ctgatcccca ggaatcgagc ggtagcattg aatgtgcggc gagcccggca
     7561 aacagcgctc gcccgcggag tccggcaaag cggcccattg cgaaccgtcg cgccggttgg
     7621 atcgctgaat aaccgaatcg aaggaactgc agtgaatgcc gcggcacgtg cggcggagcc
     7681 aagatgtcgg cgtccaaacc atcccaacaa tgagccagcc aacggaatac tctttggtac
     7741 gagactgcgt cgtggcccag gcccttcata gtttcatcca cggagcgatg gagcaaaacc
     7801 gcgctaccat cgtcaagcgg gtgcgccagg ggcgccgccg gctggatcca ttccaaccca
     7861 tgcttgtgca gaggcagcgt gcgaaaaaac ggcgagccaa gcgccaaagg gtaaactgac
     7921 gagcaaacgt catgactaaa gccgggcaga gtgagctctg ctgagcgtac gccaccccca
     7981 acggaatcgt tggcctcgaa cacgaccact gacttgcccg cttgcgcaac cgtaatcgcc
     8041 gcggccaagc cgttgggccc ggcgccgatg accactgcgt catatccttt ggatcggttc
     8101 atctcacccg gctagcctct ccgcgccttc aactcgatgt ccacaacgcg cgtcgcttcg
     8161 gtcccctgcg ggacttccat ggcggcgaat ccctagcttt aaggtcgatt gagaaaagaa
     8221 gcgagagacg ggtaccctgt aattctgtgg ggaatcaaac aaaaattgcc atacttacga
     8281 aaaaaagatt ttactgaggg agactttttc ggcggacaga gagacgacaa ttaaaagcag
     8341 cggccgaata acgaacgccc acgctttcgc ttaaggcacg cggcggcgcc gatctttgac
     8401 gatgtcgcgc actaaatctc cggctgcgcc acccaatgcg gttgcagcgc cggaaactac
     8461 gctcctcacc acactgcccg ctaaggttgg cggcgcggca tggcgagttc gatttcgttt
     8521 cggcttcaaa gcgcgtccga ccatccagcc gagaacagca aatgctccaa gtgtcggcca
     8581 cggacgcgtg cgtacgtaat aggcggggtt cagagtctgt ttcgtcgtta tttcgatttt
     8641 gtgttcgagc cgaccgagtt tctctccaag cgcgaaccga gtattttcga tgtctcgtcg
     8701 aatcgtttca aggctttctt ccatcggcac cacctctact aatgacctgt agccttctct
     8761 ctgatgtact gcgcatccgc tttcgcgccg cgcaaagtgt gctccgggat cggcttggcg
     8821 gtttgtagtt tgcgccggcc cacgaaaagg acgatgaggc cgactaccaa atagacgcca
     8881 ccgacgatcg caaaccccgc ccacgtcggc agcggaatgt actgcgcgag tgcgagggcg
     8941 ataacgatcg acaacagaac aaaagccacc gctaaaataa atacgcttat accgagtaga
     9001 atgctcgagc tgacgatctt ttttatttcc tgcgtgatct ccagtttcgc agccgtcagc
     9061 tctttcgtcg agagtgttcg agcgtcctgc acaataccgc gcattagctc gatgaccgat
     9121 tcggcgcgat cgtttcctcc acgtgtctga tatggtgggt ccatatttag ccaaattcga
     9181 gcaacgttcg tgccacactt ggaaagaaaa gccgtgactg aaacggtccc agtcacggct
     9241 ttacgattga taagaagcgc tacctcgtgc gatcgcctga gcctccccag acataaccca
     9301 agccgatatt gacgacatga tttccatgtg aacgattatc gcg
//
LOCUS       DASULZ010000551         4760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1180998, whole genome shotgun sequence.
ACCESSION   DASULZ010000551 DASULZ010000000
VERSION     DASULZ010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180998"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="VFY96_11300"
     CDS             <1..818
                     /locus_tag="VFY96_11300"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX5607095.1"
                     /translation="GELMTKRAHELGATNSHFTNPHGLTATDHYSSAKDLALLFRYAL
                     KNQTFREIVQTKFSSVTSTALVRKKVVPRRISVRNHNRLLWNYDGAIGGKTGYTHAAL
                     KCFVGAVQRNGTILIVAILGARDQWGDTKRLLEYGFDNYDILKGAPGAPGKAAAMEQQ
                     VGVRGDRMASVTMPAQEKSQIKINEGFILQVGSFRDRERAESLSRQINDKGIDAFVEK
                     TTMSPSETAYRVRVGPYPELVTAQETAQQILSKSGHRVLILPFQTGREEGDPS"
     gene            complement(862..1596)
                     /locus_tag="VFY96_11305"
     CDS             complement(862..1596)
                     /locus_tag="VFY96_11305"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5607096.1"
                     /translation="MRLHVLGCGDAFGSGGRHQSGYLFEASDRLFLLDCGPTTLLAMK
                     RAGFAPRRLDAIILSHLHGDHFGGIPFFFIEYLYQHPPNAPVTIAGPPDTRERVMELC
                     KLMYGSGKSPAQSTPFTFQILHPNESATVKGIEVFPFRVPHQTGDISLALKVTYEGRR
                     VLFSGDSAWTDEFIAHARGVDLFLCECSFFTEQPALHVNYQTLRANRADLQCKKLLLT
                     HLGEEMLARKNELEIPFAEDGMILTV"
     gene            1727..2179
                     /locus_tag="VFY96_11310"
     CDS             1727..2179
                     /locus_tag="VFY96_11310"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5607097.1"
                     /translation="MAVYKRPFFTSYTTSLWIRFLRFGLQSHKKLEGDLDKAGLTPPQ
                     FYVLATIGYAGGLPFGEIGQKMMVTVSNLTGIVDRLEEKKLVVRKRDDNDRRVVHVVL
                     TEKGAKLYKTAIPLFEKSVTEIFTVLPRPKQKELSSLLRKLNQEAPAS"
     gene            2305..3528
                     /locus_tag="VFY96_11315"
     CDS             2305..3528
                     /locus_tag="VFY96_11315"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX5607098.1"
                     /translation="MKNESNKPIGLERVRKWLYAKDLTERSSGCVLCGSCYGHGPANP
                     MEDAPGPKEKCPPYEYYRFQRHTPKSRWLMAQRVFHGLDPITPELKEVIYACTNCLMC
                     QELCGVRNDGYGPWDITVAMREEITEKEGPLEAHCSIYEGLQRHDNPWGQKKSERGAW
                     CESLGLKKLGAASAPTLLFAGCSADRPSGRSSAVALAKLLQVAGEEFVVLGADEKCCG
                     LYAFDLGFRGEYERLKDSNLATIQNAGITKVITTCGSCQRIWREYAKSDEAAFTVLHG
                     IEYIDQLVVSGRLKFTRAIRKKVTYHDSCHLGRGAGVYDAPRRVLSAIPGVELVEMPR
                     NRRWGWCCGGGGGVPEADPALAQWSAAERTNEARATGADVMLTASALCQRSFAEVTES
                     RLPVHDFLDFLQQAL"
     gene            3531..4268
                     /locus_tag="VFY96_11320"
     CDS             3531..4268
                     /locus_tag="VFY96_11320"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607099.1"
                     /translation="MLIKEAHFTPQRMLAMLGLKGDSFGSYALIPGPKERSETILKLL
                     KDPQKNFTFLDYEMYTGSLDGKRITVGNGGRYAPDTAITTEILCGAGAKNLIRVGSCG
                     SLQENIQIGDLLIVTGALRGEGTSRYYVPENFSTVAHSEIINALKRAADSLNVRYHLG
                     WIFTTDALFQETPELIQQLNEQRVSAIDMVTSTFLTVAQVRGAKAGAILAVSDECLYC
                     KFGFRDPRFQEAEKKTIEVARAALQYL"
     gene            4286..>4760
                     /locus_tag="VFY96_11325"
     CDS             4286..>4760
                     /locus_tag="VFY96_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607100.1"
                     /translation="MISIQQSPLFEPVLWNGNGFKILDELLAPERIEYIHITQVAQAL
                     DAVRQMKTRAFGQVLTFLYSGALLAGAYRGDEAAPLRQEIAAMTEAFCSARPTFDFRG
                     LGAFFDECFSKLKSDGALGETIACRAREFGHEIVRARQTRAKRAASILPQRAQILT"
BASE COUNT         1125 a         1274 c         1365 g          996 t
ORIGIN      
        1 ttggtgagct gatgacgaaa agagcacacg agctcggagc gaccaatagc cacttcacca
       61 atccccatgg cctcaccgcg acggatcact attcgagcgc aaaggacctc gcgctcttgt
      121 tccgatatgc actgaaaaac cagacctttc gcgaaatcgt ccaaacgaaa tttagttcgg
      181 tgacgagcac ggcattggta cgtaaaaaag ttgtgccgcg gcgcatttcg gtcaggaatc
      241 ataatcgttt gttgtggaat tacgacggcg cgatcggcgg caaaaccggc tatacgcatg
      301 ctgccctaaa gtgctttgtg ggcgcagtac aacggaatgg cacgatcttg atcgttgcca
      361 ttctaggcgc gcgcgatcaa tggggcgata ccaagcggct tttggaatat ggcttcgata
      421 attatgacat cctcaagggc gcgcctggag cgccggggaa ggcagccgcg atggagcagc
      481 aagtcggcgt ccgtggcgac cggatggcgt cagtcactat gccggcacaa gaaaaaagcc
      541 agattaagat caacgagggg ttcatcttac aggtgggatc ttttcgtgac agagagcgag
      601 cggaatccct ttcgcgccag atcaatgata aaggcatcga cgctttcgtc gaaaaaacta
      661 ccatgagtcc gagcgaaacc gcgtatcgcg tccgggtcgg gccgtatccc gagctcgtta
      721 cggcgcagga gaccgctcag cagattttga gcaaaagcgg ccatcgggtt ttgatcctac
      781 cttttcaaac agggcgggaa gaaggcgatc caagctgacg cgcgacgggc aacctcgcaa
      841 attgaccatc gcagtccctc tctagactgt taaaatcatt ccgtcctcgg cgaacggtat
      901 ttccagttca ttttttcggg ccagcatctc ttcgcctaaa tgggtgagga gcagtttttt
      961 gcactgaagg tctgcgcggt tcgccctcag tgtttgataa ttgacgtgca gcgcaggttg
     1021 ttcggtaaaa aacgagcact cgcagagaaa tagatcgacg ccgcgagcgt gcgcgatgaa
     1081 ctcatccgtc catgctgaat caccggaaaa aagaacccgg cggccttcat aggtgacctt
     1141 cagggccaga gaaatgtctc cggtttggtg cggtactcgg aaaggaaaga cctcgattcc
     1201 ctttacggtc gccgactcat ttggatgcaa aatttgaaag gtgaatggcg tgctttgggc
     1261 cggacttttg ccgctcccgt acatcaactt gcaaagttcc atcacccgtt cgcgagtgtc
     1321 cggcggtccc gcgatcgtga cgggggcatt gggcgggtgt tgatagagat attcgatgaa
     1381 aaaaaatgga atcccaccga aatgatcgcc gtgtaaatga ctcagaataa ttgcgtcgag
     1441 acggcggggc gcaaagccgg cgcgtttcat ggcgagtagt gtggtcggcc cacagtcaag
     1501 taagaacagc cgatcactcg cttcaaaaag gtagccgctc tgatgacgac cgccgctgcc
     1561 gaacgcatcg ccgcagccga ggacatgaag tctcattgaa ttgttaccaa cgagtgtaaa
     1621 ggcatggatt ttcgactaac actctgctcg tgccgatgca acgcgatgcg tccgagttgg
     1681 cgcaaataaa gttgcttttt cacggccggc cgactacagt caacgggtgg ctgtttacaa
     1741 gcgcccattc ttcacttcat acaccaccag tctttggatc cggtttcttc gtttcggttt
     1801 gcagtcgcac aaaaagctcg aaggtgattt ggataaagcc ggcctcacgc caccgcagtt
     1861 ttacgtgtta gcgacgattg gctacgcggg cgggcttcct ttcggtgaaa tcggccagaa
     1921 gatgatggta accgtcagca acctcaccgg cattgtcgac cggctcgagg aaaaaaagtt
     1981 ggtggtgcga aagcgcgacg acaacgaccg ccgtgtcgtg catgttgtgc tgacggagaa
     2041 gggcgcgaaa ctgtataaaa ccgcgattcc gctattcgaa aaaagcgtca cagaaatttt
     2101 taccgtgctt ccgcgaccca agcagaaaga actctcgtca ctcctacgca agcttaacca
     2161 agaagctccg gcgagctgaa tatatctgcg gtttacaaat cgaagatttt cccgctacgg
     2221 ccgaagatta gcgtcagccg caataagttg acagatggaa tgggcgagct ataaaggttt
     2281 aaaccttaac taatttccaa gattgtgaaa aacgaaagca ataagccaat tggcctcgaa
     2341 cgggtcagga agtggcttta tgccaaagat cttaccgaac ggagcagcgg ctgtgttctc
     2401 tgtggctcct gttatggcca tgggccggcc aatccgatgg aagatgcgcc aggcccaaag
     2461 gaaaaatgcc cgccctacga atactaccgc tttcagcgcc acacgccgaa gtcacgctgg
     2521 ctgatggcac agcgggtttt tcacggtctc gatccgatta ctccggagct aaaagaagtc
     2581 atctatgctt gcacgaactg cctgatgtgt caggaacttt gcggggtgcg caatgatggc
     2641 tacggcccgt gggatattac cgtcgcaatg cgcgaggaaa tcacagaaaa ggaaggcccg
     2701 ctcgaagcgc attgctcgat ctacgagggg ctgcaacggc acgataaccc atggggccag
     2761 aaaaaatcgg aacgcggtgc gtggtgcgaa agtcttgggc taaaaaaact cggggcggcg
     2821 agtgcgccaa ctttgctttt tgccggttgc tcggcggatc gcccgagcgg ccggagcagc
     2881 gctgtggcgc tggcgaagct gcttcaggtt gccggcgagg agttcgtcgt tcttggcgca
     2941 gacgaaaaat gctgcggcct gtatgccttc gatctcggat ttcgtggcga atacgagcga
     3001 ctgaaggatt cgaatctagc caccatccag aatgccggaa tcaccaaagt cattacgacg
     3061 tgcggcagtt gccagcgcat ctggcgcgag tatgccaaat cagacgaggc tgcgtttacg
     3121 gttctgcatg ggatcgaata catcgaccaa ctggtagtgt cagggcggct gaagttcacc
     3181 cgcgcaatac gcaaaaaagt gacctaccat gattcctgtc atctcggtcg cggcgcggga
     3241 gtttacgacg ccccgcgtcg ggtgctcagc gcaattccgg gtgttgaact ggtggaaatg
     3301 ccgcgtaacc gccgctgggg atggtgctgc ggcggtggtg gcggagttcc tgaggcggat
     3361 cccgcgctgg cacaatggag cgcggcagag cgcaccaacg aggcgcgcgc gaccggtgcc
     3421 gatgtcatgc tgaccgccag tgcactgtgc cagcggtcat ttgccgaggt gacagagtcc
     3481 cgattgccgg tgcacgattt tctggatttt cttcagcagg cactctgatc atgctcatta
     3541 aagaagctca tttcacccca caacgcatgc tcgcgatgct gggactgaag ggagactcct
     3601 ttggctccta tgctctgatt cccggcccga aggagcgcag tgaaacgatt ctcaagctgc
     3661 tcaaggatcc acaaaagaat ttcacgtttc ttgactacga gatgtacacc ggctccctgg
     3721 acggtaaacg gattacggta gggaatggtg ggcgttatgc gccggatacc gcgatcacga
     3781 cggaaattct ttgcggcgcc ggagcaaaaa atctgattcg ggtcggcagc tgcggttcgc
     3841 tacaggaaaa cattcaaatc ggcgatctgc tgattgttac cggcgcgctc cgcggcgaag
     3901 gcacctctcg ttattatgtg ccggaaaatt tctccacagt tgcccactcc gaaatcatca
     3961 atgcgctaaa gcgagccgcc gacagtttaa acgtgcgtta tcacctcggc tggatcttca
     4021 ccaccgacgc gctatttcag gaaaccccgg aactgattca acaattgaac gagcagcgag
     4081 tctcggccat cgatatggtg acctccacgt ttctaaccgt cgcgcaggtg cgcggggcaa
     4141 aagcgggagc gattctcgcg gtctcggacg aatgcctcta ttgcaaattt ggctttcgcg
     4201 atccgcgctt tcaggaagcc gagaaaaaga ccatcgaagt ggcacgagcc gcattgcaat
     4261 atctgtaact cggcgacaga acggaatgat cagcattcag caaagcccgc tgttcgaacc
     4321 ggtgctctgg aacggcaacg gctttaaaat cctcgatgag ctcctggctc cggagcggat
     4381 cgagtacatt cacatcaccc aagtcgcgca ggcactcgat gcggtgcggc agatgaaaac
     4441 ccgagctttc gggcaagtgc tcacgttttt atacagtggg gcactgctgg cgggcgcgta
     4501 ccgcggtgat gaagccgcgc cgctgcgcca agaaatcgcg gccatgacgg aagcgttttg
     4561 cagcgcccga cctaccttcg acttccgcgg cctcggcgcg tttttcgacg agtgcttcag
     4621 caaattgaag tcggatggtg ccctgggcga aacgatcgcc tgccgcgcgc gcgaattcgg
     4681 ccatgagatc gttcgcgccc ggcagacgcg cgccaagcgt gcagcctcga tcctgccgca
     4741 gcgtgcgcaa atactgaccc
//
LOCUS       DASULZ010000552         2664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_367868, whole genome shotgun sequence.
ACCESSION   DASULZ010000552 DASULZ010000000
VERSION     DASULZ010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367868"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /gene="ahcY"
                     /locus_tag="VFY96_11330"
     CDS             complement(<1..1034)
                     /gene="ahcY"
                     /locus_tag="VFY96_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX5607101.1"
                     /translation="MAGKKHDVKDLRLADGGRKRVLWAAQDMPVLRRVKERFEKEKPL
                     RGMRFSACLHVTAETANLVQALKAGGADVLLCASNPLSTQDDVAASLVQHDKVPVFAI
                     KGEDNKSYYSHLRAALDHQPVITMDDGADLVSLLHTDYAHLASNLIASMEETTTGVIR
                     LRALERDGALKIPVIAVNDAATKHLFDNRYGTGQSTIDGVVRATDMLIAGKRVVVAGY
                     GWCGKGVASRARGMGAKVIVTEVDPVRALEAAMDGLEVMTMKEAARIGDLFITLTGDM
                     HVIAEEHLKAMKDGAVICNSGHFDIEIDLKALKRLSAKVEKNVRNCVDAYVLPGGKRI
                     YLIGEGRLVNL"
     gene            complement(1071..2249)
                     /gene="metK"
                     /locus_tag="VFY96_11335"
     CDS             complement(1071..2249)
                     /gene="metK"
                     /locus_tag="VFY96_11335"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX5607102.1"
                     /translation="MAVKDFLFTSESVSEGHPDKLCDQVSDAILDAHLAGDPNSRVAC
                     ESLAKTGMVVVAGEITSNAKVSYVDVVREVVREIGYTDSAMGFDGHTCAILTAIEQQS
                     PDISQGVTEGEGLHKEQGAGDQGMMFGYACDETPELMPFPIQMAHSLVHYLAKVRKGG
                     EAYFLRPDSKSQVTVEYRDGKPVKVKNVVISTQHSPDVDHSKLKETIIDGVIKAVIPP
                     EYLDKDTVFHVNPTGRFVVGGPQGDCGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVD
                     RSAAYMARYVAKNIVAAELAQRCEVQLAYVIGVAQPVSVLVDTFGTGVVPEGRIAKIV
                     QESFDLSPKGIIQALDLKRPIYRPTAAYGHFGRSPEDGLFTWERTDRAADLKKAAGRL
                     "
     gene            complement(2358..>2664)
                     /locus_tag="VFY96_11340"
     CDS             complement(2358..>2664)
                     /locus_tag="VFY96_11340"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX5607103.1"
                     /translation="GEEFVIILPEITLDSAHQRLEQLRLELQRIAVRQDGQSVTSVTV
                     SIGMALYPDHGRTNHGLLQAADQALYKAKELGRNCVVIASDGMIQNESALDWSLRSS"
BASE COUNT          535 a          816 c          713 g          600 t
ORIGIN      
        1 agattcacca accgtccttc gccgatcaaa taaatccgtt tgccaccggg caagacgtag
       61 gcgtcgacgc agttgcggac atttttttct actttggcgg aaagcctctt gagcgccttc
      121 agatcgattt caatgtcgaa atgacccgag ttgcagatca ccgcgccgtc tttcatcgct
      181 ttgagatgtt cttcggcgat cacgtgcata tcgccggtga gcgtgatgaa gagatcgccg
      241 atccgcgccg cctctttcat ggtcatgact tccaggccat ccatggccgc ttccagcgcg
      301 cgcaccggat cgacttcggt gacgatcact ttggcgccca tcccgcgggc gcgggaagcc
      361 acgcccttgc cgcaccaccc gtagccggcg accaccaccc gtttgccggc gatgagcatg
      421 tcggtcgccc gcacgacgcc gtcaatggtc gattgtcccg tgccgtaacg attgtcaaaa
      481 agatgcttgg tcgccgcgtc gttcacggcg atgaccggga tttttagcgc gccgtctctt
      541 tccagagcgc gcaagcgaat aacccccgtg gtggtctctt ccatgctggc aattaaattg
      601 gacgcgagat gcgcataatc ggtgtgcagc agagaaacca gatcggcgcc gtcatccatt
      661 gtgatgacgg gttgatgatc gagcgccgcg cgcaggtgac tgtagtaact cttgttgtct
      721 tcgcccttaa tcgcaaatac cggcactttg tcatgctgaa ccagtgacgc cgcgacgtcg
      781 tcctgagtcg agagcggatt cgacgcgcac agcaacacat ccgcgccgcc cgctttgagc
      841 gcctgcacca agtttgcggt ctccgctgtc acgtgaaggc aggccgagaa gcgcatgccg
      901 cggagcggtt tttctttttc gaatcgctcc ttcactcgcc gcagcacagg catgtcttga
      961 gcggcccaga ggactcgctt gcgcccgcca tccgcaaggc gcaaatcttt aacgtcgtgt
     1021 ttcttacccg ccatcgtaac cacttcctcc gcgtaaattc tttgggtgag ttaaagccgg
     1081 cctgcagcct ttttgagatc ggccgcccga tcggttcttt cccaggtaaa gaggccgtct
     1141 tcaggactgc gcccgaaatg gccgtaggcc gccgtcggac ggtagatggg tcgtttgaga
     1201 tcgagggctt gaataatgcc tttcggactg agatcaaaac tttcctgaac gatttttgcg
     1261 attctgcctt caggcacaac cccggtgccg aaggtgtcga ccagcaccga aaccggctgc
     1321 gctacgccga ttacgtaagc tagttgcacc tcacagcgct gcgccaattc tgccgcaacg
     1381 atatttttcg ccacatagcg cgccatgtag gcggcgctac gatcgacttt cgatggatct
     1441 tttccagaga aggcgccgcc accgtggcgc cccatcccgc cataggtatc gacgatgatt
     1501 tttcgccccg tgagaccgca atcgccctgc ggcccgccga ccacgaaccg tcccgtcggg
     1561 ttcacatgaa aaaccgtgtc cttgtccagg tactcgggcg ggatcacagc cttgatcacg
     1621 ccgtcgataa tggtttcctt cagcttgcta tggtcgacat cggggctgtg ttgcgtggaa
     1681 ataacgacat ttttcacctt gaccggcttg ccgtcgcgat actcgaccgt cacttgtgac
     1741 ttgctgtcgg gccggagaaa atacgcttca ccacctttcc gaactttcgc caggtaatgc
     1801 accaagctgt gcgccatctg aatcggaaac ggcatcagtt cgggcgtctc gtcgcaagcg
     1861 tagccgaaca tcatcccctg atcgcccgcg ccctgctctt tatgtaaccc ttcaccttcg
     1921 gtcactccct gggaaatatc cggcgattgc tgctcgatgg cagttaaaat cgcgcatgta
     1981 tggccgtcaa atcccatggc ggaatccgtg taaccgatct cgcgcacgac ttcgcggacg
     2041 acatcgacat aactgacctt ggcattgctc gtgatctccc ccgccaccac caccatgccg
     2101 gttttcgcga gactttcgca ggccacgcgg ctgttgggat ctcccgccag atgagcatcg
     2161 aggatcgcat cggaaacttg gtcgcagagt ttatcgggat gcccttcgga gacggactcc
     2221 gatgtgaaca agaaatcttt gacagccatg agatgacctc caacggggcg cgaacaatca
     2281 ctcctgtata gcgaatacag ggggtctatt caaccttact cttgtgctct taatggcgga
     2341 aaaggcggcc agtccgctta ggaactacga agggaccaat ccaaggcgct ttcgttctgt
     2401 atcatcccgt cggacgcgat cacgacgcaa ttccgaccca attccttggc tttatataga
     2461 gcttgatcgg cagcctgcag caagccgtgg ttcgtccggc cgtgatcggg atagagcgcc
     2521 ataccgatcg aaaccgtcac gctggtgacc gattgcccgt cttggcggac cgcaatgcgc
     2581 tgcagctcga gccgaagctg ctcgagccgc tggtgcgcgc tatccaaggt gatttccgga
     2641 aggataataa caaattcttc gccg
//
LOCUS       DASULZ010000553         3070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_572280, whole genome shotgun sequence.
ACCESSION   DASULZ010000553 DASULZ010000000
VERSION     DASULZ010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572280"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1347)
                     /locus_tag="VFY96_11345"
     CDS             complement(175..1347)
                     /locus_tag="VFY96_11345"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX5607104.1"
                     /translation="MKIRAEQNSDPDPFPRFSTTEYTRRYTAIREAMDRENLAALLIS
                     GARGSSEVHYLSNYLAQSSCWLLFPLNDSPVVFIHFFNHQPCANAQSVIEDVRWYGPA
                     PMPTVVDELRKRGMAKSNIGLVSMRVMPYGHVTALQRAFPEAEFVEFGPQFNRIRQVR
                     SEEELVYLRKSGYLTDLACEALERDLRAGLTEHDVLSIVYGGYIKNGGDPGIHFIATT
                     NMDQPDRFVPWQKQTARVLEAGSVVITELTVSYWGYSTQIHRPFAIAREPSPTYRKLF
                     DTARQCFENIRKICQPGISSDEIVAASSVIEENGFTSYDSVFHGEAGKSPELGTKSSA
                     HPLEPWTLQENMVHVIQPNPVTRDFRAGLQLGAAVVVKPSGGELLHNYPFKFPVCG"
     gene            complement(1362..>3070)
                     /locus_tag="VFY96_11350"
     CDS             complement(1362..>3070)
                     /locus_tag="VFY96_11350"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5607105.1"
                     /translation="HDSVVDGVHLCMRRLKALRLNTMAALDLAATADLNRLRRFGPEQ
                     LQSLGRLAHPMIRRHKEPGFVRCSWDESLEFVCRAIHDTASHAMGFLATPHGRTNEIY
                     YVFQKLARILGSNNIALCSRPSHDNAVSGLKSTLGYGSPTCSLSDWIGTDLLIIFGAD
                     ELSDYPVTKKYMRRARKAGTRIVAVTPLARAKTEDDWRSVVRSTVPGRVSTPYRFQVR
                     AGGEIAFINGILKTLIANDQVDMEFIDKHTARFDALREALQSQSWEMLEQCSGVSRTA
                     MQEFAIQYGAARSAVFVYTGTFTEGESASTKVKAIVNLALARGMIGRDKCGIMPIGAQ
                     SSAHAGGECGAEPDQFPGGFRVNENGARRLSNLWRHPVPSNPGLTLSEMIAAAYAYQI
                     KFFYFLGSDPLEAWTEDSYAAEALRRVPVRIYQDLVLKPSMLLDSAGAVVVLPAQTRY
                     EQRTGGTCTSTERRIRFTPELKGHKIGEARPEWEILSSIGRKSMPNGDLLFPFNSSQT
                     IRDEMNRVMPIYQGVEKITKEGDQLQWGGPQLYHDGFSMMPGNRALFSALRPPARDSR
                     NQ"
BASE COUNT          656 a          851 c          879 g          684 t
ORIGIN      
        1 ctgaacgagc cggtggtcat gatcactttt gtgagtggct gggctgccat gacgggtgaa
       61 acagcaccca tggcagcgat cagcgcagcc actagaagtt ttgtcttggt gatcatgata
      121 tttccgcgct accgccgaga cgattggaac cgactgagca gatcattcct gcattcaccc
      181 gcataccgga aacttgaatg gatagttgtg taacagctcg cccccgctcg gtttcacgac
      241 caccgcggcg ccaagctgta atcctgcgcg aaaatctcgt gttaccggat tcggctggat
      301 gacatgaacc atgttctcct gcaatgtcca cggttccagt ggatgggccg atgacttcgt
      361 cccgagctcc ggtgatttcc cggcctcgcc atgaaacacg ctgtcgtaag aagtaaagcc
      421 attttcttcg atgacggagg acgccgcgac aatctcgtcg ctgctgatgc cgggctggca
      481 gatcttgcga atgttttcga agcattgccg cgcggtgtcg aaaagctttc gataggtagg
      541 cgatggctca cgggcgatcg cgaacggacg atgaatctgt gtcgagtagc cccaatagct
      601 caccgttagc tcggtgatta cgacgctacc ggcttcgagc acgcgcgcgg tttgcttttg
      661 ccacgggaca aagcggtctg gctggtccat gttggtggtc gcaatgaagt gaataccggg
      721 atcgccgcca ttcttaatat agcctccgta gacaatcgag agcacgtcgt gttccgtcaa
      781 gccggcgcgt agatcgcgct cgagggcttc gcaggcgaga tcggtcagat aaccgctctt
      841 gcgcaggtag acgagctcct cttcgctgcg cacctgacga atccgattga actgcggacc
      901 gaattcgaca aattcagctt cgggaaaagc acgttgcagc gcggtcacat gtccgtaggg
      961 catcacacgc atgctcacca ggccgatatt gcttttggcc ataccgcgct tgcgcaattc
     1021 gtcgacgact gtcggcatcg gtgctggccc gtaccagcgt acgtcttcga tgaccgactg
     1081 tgcgttggcg cacggctgat gattgaagaa gtggatgaaa accacaggac tgtcgttgag
     1141 gggaaataat aaccagcaag aggattgtgc aagataattc gagagatagt gaacttccga
     1201 agaaccgcga gcgccggaga tcagtagagc cgctaggttc tctctatcca tcgcttcacg
     1261 gatcgcagtg tatcggcgcg tgtactcggt ggtggaaaaa cgcgggaatg gatctggatc
     1321 cgaattctgt tcagcacgaa tcttcatcgt cttcccgaaa attattgatt ccttgaatct
     1381 cgggcgggcg gccggagtgc gctgaaaagt gcacgattgc cgggcatcat gctaaaccca
     1441 tcgtgataaa gctgcggacc tccccactga agttgatctc cttctttggt gattttctcc
     1501 acgccttggt agatcggcat cacgcggttc atttcgtcac gaatggtctg actactattg
     1561 aaaggaaaca gcaggtcgcc attgggcatc gacttacgcc cgatcgagct caaaatttcc
     1621 cactccggac gtgcctcgcc gatcttatgg cccttaagct ccggcgtgaa gcgaatccga
     1681 cgttcggtgc tcgtgcacgt gccaccggtc cgttgctcgt agcgcgtttg tgccggcaaa
     1741 acaaccacgg ctccggcgct gtcgagcagc atcgagggct tgagcacgag gtcctggtat
     1801 atacgtaccg gaacgcgtct caatgcctcg gcagcgtagc tgtcctccgt ccacgcttcg
     1861 agcggatcgc taccaagaaa ataaaaaaat ttgatttgat atgcgtaagc cgcagcaatc
     1921 atttccgaga gtgtcaggcc cggattggag ggtaccgggt ggcgccagag gtttgacaag
     1981 cggcgggcgc cgttctcgtt gacgcgaaac ccgccgggga attggtccgg ctcggccccg
     2041 cattcgccgc ccgcatgcgc gctgctctgg gcgccgatcg gcataatgcc gcatttgtca
     2101 cgcccgatca taccgcgcgc cagcgccagg tttacaatcg ctttgacctt ggtactggca
     2161 gattcgcctt cggtaaaagt gcctgtgtag acgaatacgg ctgatcgtgc cgcgccatat
     2221 tgaatcgcga attcctgcat tgcagtgcgc gaaacgccgg agcattgctc gagcatttcc
     2281 cacgactgac tctgcagtgc ctcgcgaagg gcgtcgaaac gtgccgtgtg cttatcgatg
     2341 aactccatat cgacctgatc gttcgcgatg agcgtcttta ggataccgtt gataaaagcg
     2401 atctcgccgc cggcgcgaac ttgaaaacga tagggcgtcg agactcttcc aggcacggtg
     2461 ctcctgacga cagacctcca gtcgtcctcc gttttcgctc gcgctagcgg agtcactgca
     2521 acgatgcgtg ttcccgcctt tcgcgcacgg cgcatgtact tctttgtcac cggataatca
     2581 ctaagctcat cggcaccgaa aatgatcagc aggtcagtcc cgatccagtc cgaaagcgaa
     2641 caggtgggcg agccatatcc gagtgtagat ttcagtcctg aaaccgcatt gtcgtgggag
     2701 gggcgggagc agagagcgat attgttgcta ccaagtattc gcgccagttt ttgaaacacg
     2761 taatagattt cgttggttcg tccgtgcggc gtcgcgagga atcccatcgc atgggatgcc
     2821 gtgtcgtgaa tcgcgcgaca gacaaactcg agactttcat cccaggagca tcggacgaaa
     2881 ccgggttctt tgtgccggcg aatcattgga tgtgccaagc ggccgaggga ctggagttgc
     2941 tcaggaccga accgtcgcaa tcgattcaag tccgctgtgg ccgccagatc gagtgccgcc
     3001 atcgtgttca gcctaagtgc tttgagccgg cgcatacaga ggtgaacgcc gtcaacgaca
     3061 ctgtcatgga
//
LOCUS       DASULZ010000554         4515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1485064, whole genome shotgun sequence.
ACCESSION   DASULZ010000554 DASULZ010000000
VERSION     DASULZ010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485064"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1420
                     /locus_tag="VFY96_11355"
     CDS             <1..1420
                     /locus_tag="VFY96_11355"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HEX5607106.1"
                     /translation="KRLKAQELASTAQDLTRNQERLMDALEKLQSGDKNLDAIMKQIS
                     DLANTLQSLQQALSQLAQQMPDDFMNAENMQGLGFDEMFRALQEIQKKFQEGDIEGAR
                     QLARELFNQMAQMVAALQNAQRSTMASGMGRMQGEMQRQSSELQEIAREQQEILMKTE
                     GMNNAALTERDAALKNKLDRFLEKAITELGQLTELFPDREGPDDPSLLTNLDDATMNS
                     LLKNLIAKLSNKDFPGFKEGDGLAHKELSKKRSRAQEARAQRAEKSLDNIKSELESML
                     NQPGQALTDAEKRNLRDLANRQDMLRERTNELHEKLESLFQLFPQLDPKIVQNVGEAG
                     TSMGGAHERLGQLDSRGAVPPEQTALDRLSQSQQQMQSAMQQMAQRGQIGNMPMTRLF
                     RQGRFLPYGSMMPLPGMPQFPDFNIENGFSGLDTEKFRLPGKDDYKAPRNFREEILDS
                     LKHGVPPQMKDQIERYFKNLSE"
     gene            1498..3084
                     /locus_tag="VFY96_11360"
     CDS             1498..3084
                     /locus_tag="VFY96_11360"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5607107.1"
                     /translation="MALSLGLVVLLCVLSPMGSFGAEADPKFKAGEELLDAGRIAQAE
                     EFAAKALKEEPKSPAALDFDARVKFYQGRYAEALAAVDRALAIDSSNERRQALKLFSQ
                     LTLDVHKALKPFESAHFVLFADEKRDGILVPYALETLEKTYQTTGAELGFFPKDKVRV
                     EIAPDATSFNAISTLSLRDIEETGAVGICKFNKLMLISPRALSFGYRWLDSLSHEYQH
                     YAIVSLSNNQAPIWLHEGMARFYETRWRKPAPAKDAAEDYLTPANQTLLVHALEKNQF
                     VGFKKMEPSLIYLETPEQVQLAYAEAASAVDFINQSKGRDGVRELLKTLNDKPTPEAI
                     EKVYGISFDNFESKWKGFLKSKGLKEIEGSRVRKLKVKKDQREDEEVVELKEIQSAVA
                     RNRTYLADQLLAKGRALAAANEYQRALQASPRSPVILYKLGRIMVETNRPDDALPLFQ
                     QALEIDPDNVNIYVQLGRAEHAKKNFKAARAALEEAIQINPFNPLIYRLLGDAYSALG
                     DQEKARSARTALERLSASRN"
     gene            3099..4127
                     /locus_tag="VFY96_11365"
     CDS             3099..4127
                     /locus_tag="VFY96_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5607108.1"
                     /translation="MELPEIQEHEIAEIEEFAAKRDSMLGEIRKVIVGQDHVIEEVLI
                     ALFAKGHCLLVGVPGLAKTLLISTLAEILDLEFNRIQFTPDLMPSDITGTDILQEDAG
                     TGKRSFQFLRGPVFTNILLADEINRTPPKTQAALLQSMQEYKVTAGGSTFALALPFFV
                     LATQNPIEQEGTYPLPEAQLDRFMVNIEIGYPNFEDEVQIVMQTTSTNNPAPRKIMDA
                     AAILHYQELVRKVPVSPFVVSYAVALAQRSRPQSPDASPYVKDYVEWGAGPRASQYLI
                     LGAKARTILQGRYAVSIEDIQALAPSVLRHRIVPNFKAQGEGLSSSDIIGRLLTEVKP
                     SGDGKAGR"
     gene            complement(4301..>4515)
                     /locus_tag="VFY96_11370"
     CDS             complement(4301..>4515)
                     /locus_tag="VFY96_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607109.1"
                     /translation="ECFKLAIDTAQKQKARALELRAAIPLARMWQSTGKTREAHRMLT
                     RIYGWFTEGFDTPDLKQARELLDELS"
BASE COUNT         1072 a         1285 c         1267 g          891 t
ORIGIN      
        1 caagagactg aaagctcagg aactggcctc cactgcgcaa gacttgacgc gcaatcagga
       61 acggctgatg gacgctttgg aaaagctaca aagcggcgac aaaaacctgg acgcgatcat
      121 gaaacagatc tcggatctgg ccaacacgct gcaaagcttg cagcaggcat tgtcgcaact
      181 cgcccagcag atgcccgatg atttcatgaa cgcggagaac atgcagggcc tcggcttcga
      241 tgaaatgttc cgggccttgc aagagatcca aaagaaattc caggagggag acatcgaagg
      301 cgctcggcag ctggcgcgag agttgttcaa tcagatggcg cagatggttg ccgcgttaca
      361 aaatgcccag cgctccacga tggcctccgg tatggggcgc atgcaaggcg agatgcagcg
      421 ccagagcagt gagctccagg agatcgcccg cgagcagcag gaaatcctga tgaaaaccga
      481 gggcatgaac aacgccgcct tgaccgagcg cgacgccgcc ttgaaaaaca agctcgatcg
      541 gtttcttgaa aaggcgatta cagagttggg ccagctcacc gagctttttc ccgatcgcga
      601 agggccggac gatcccagtc tgctgaccaa tctcgatgac gcgacgatga attcgctttt
      661 gaagaatttg atcgccaagc tcagtaacaa agacttcccc ggatttaagg agggggacgg
      721 cttggctcat aaggagcttt cgaaaaagcg cagccgagcg caggaagcgc gggctcagcg
      781 ggccgagaag agcctcgaca acattaagtc cgagcttgag tcgatgctga atcagccggg
      841 gcaagcgctc acggatgccg agaagcgcaa ccttcgagat ctcgcgaacc ggcaagatat
      901 gctgagagag cgaaccaacg aactgcacga aaaactggag tccctgtttc agcttttccc
      961 gcagctcgac cccaagatcg tacaaaacgt tggcgaggcc ggaacctcga tgggcggcgc
     1021 ccatgaacgt ctcggtcaac tggattcccg cggcgcggtg ccgccggagc aaaccgctct
     1081 cgatcgcctg tcacagtccc agcagcagat gcaatcggcg atgcaacaaa tggcgcagcg
     1141 cggccagatc ggcaacatgc cgatgacgcg gctgttccgc caggggcggt ttctcccgta
     1201 tggttcgatg atgccgcttc ccggcatgcc gcagtttccc gatttcaaca ttgaaaacgg
     1261 cttcagcggt ttggataccg aaaagttccg cctacccggt aaagacgact acaaggcgcc
     1321 gcgtaacttc cgcgaagaaa ttctcgactc actgaagcac ggtgtgccgc cgcaaatgaa
     1381 agatcagatc gagcgttact tcaaaaatct ttccgagtga tcctggtgtc ctgccataga
     1441 gcttcttcca attattgggc gtggtcgttg ggtcgtcgtc ggcgccaggc gtcgaacttg
     1501 gcgctgtcgc ttggcctggt cgttctgctt tgtgttttgt cgccgatggg ctctttcggc
     1561 gccgaagccg atccgaagtt taaagccggc gaagagttgc tcgacgccgg acggattgcg
     1621 caggcggaag agtttgcggc caaagcgctc aaggaagagc caaagtctcc cgccgctttg
     1681 gacttcgacg cccgagtcaa attttaccaa ggacgctatg ccgaggcttt agcggctgtc
     1741 gatcgagcgc tcgccatcga ttcgtcgaat gaaagacgcc aggcgctaaa gcttttcagc
     1801 cagcttaccc tggatgtcca taaagcactg aagccatttg agagcgcgca ctttgtgctt
     1861 ttcgctgatg agaagcgcga tggcatcctg gtcccttacg cgctggagac gctggaaaaa
     1921 acctatcaaa caaccggcgc cgagctcggc ttttttccca aagacaaagt ccgcgtggaa
     1981 atcgcgcccg atgccaccag cttcaatgcg atttccactc tgtcgctgcg tgatatcgag
     2041 gaaacgggcg ccgtcggcat ctgcaagttc aacaagctga tgctcatttc accgcgcgcg
     2101 ctgagctttg gctaccgctg gctcgattcc ctcagccacg aataccagca ctatgcgatc
     2161 gtctccctga gcaacaacca ggcgccgatt tggcttcacg aaggcatggc gcggttttac
     2221 gagacccgct ggcgcaagcc ggcgcccgcc aaagatgccg ccgaggatta tctcacgccg
     2281 gccaatcaga ccttgctcgt ccatgctctg gagaaaaatc aattcgtcgg tttcaaaaaa
     2341 atggaaccct cgctcattta tttggaaaca ccggagcaag tccaactcgc ttacgccgag
     2401 gccgcttcgg ccgtagattt catcaaccag agcaagggac gcgatggcgt gcgcgagttg
     2461 ctcaagacac tgaacgacaa gcccacgccg gaagccatcg aaaaggtcta cgggatctct
     2521 ttcgacaact tcgaaagcaa atggaaaggc tttctcaaaa gcaaagggct caaggagatc
     2581 gaaggcagcc gcgtacgcaa gctcaaggta aaaaaagatc agcgggaaga cgaggaagtg
     2641 gtggagctga aggaaatcca gtcggcggtg gcccgcaacc ggacgtacct cgccgatcag
     2701 ctgctcgcca agggtcgtgc gctggcggcg gctaatgaat atcaacgcgc cttgcaggcg
     2761 agcccgcgtt caccggtgat tctgtacaag ctcggccgca tcatggtcga gaccaatcgc
     2821 ccggacgacg ccttgccgct gtttcagcaa gctctcgaga tcgatcctga taacgtcaac
     2881 atctatgtgc agttgggtcg ggctgagcac gccaagaaga atttcaaagc cgctcgcgcc
     2941 gcgctcgagg aggcgatcca gatcaatcca tttaacccgc tcatctatcg tttgctcggc
     3001 gatgcctaca gtgcgctcgg agatcaggaa aaagcacgct cggccaggac agcgctggag
     3061 agattgagcg cgagcagaaa ttagccagaa ggaacgtcat ggagttaccc gagattcagg
     3121 agcacgaaat cgccgaaatt gaagagttcg ctgccaagcg cgacagcatg cttggcgaaa
     3181 tccgcaaagt catcgttggc caggatcacg tcatcgaaga agttttgatc gcgctgtttg
     3241 caaaggggca ttgcttgctg gtcggcgttc ccgggctagc gaaaacgctt ttgatcagca
     3301 ccctcgcgga aatcttggat ctcgaattca atcggattca gttcacgccc gatttgatgc
     3361 cttcggacat caccggaaca gatatcctgc aggaagatgc cggcaccggc aaacggagct
     3421 ttcaattcct caggggaccg gttttcacca atattctgct ggcggacgaa atcaaccgca
     3481 cgccgccgaa aactcaagcc gcgcttttgc agtccatgca ggaatacaaa gtcaccgccg
     3541 gcggcagcac gttcgcgctg gcgctgccgt tttttgtctt agcaacgcaa aatcccatcg
     3601 agcaggaagg gacctacccg ctgcccgaag cacagctcga tcggttcatg gttaacatcg
     3661 aaatcggcta tcccaacttc gaagatgaag tgcagatcgt gatgcagacg acctcgacga
     3721 ataatccggc gccgcggaaa attatggacg ctgcggccat tttgcattat caggagctgg
     3781 tgcgaaaagt tccggtttcc ccgtttgtcg tttcctacgc cgtggcgctc gctcagcgct
     3841 cgcgccctca gagcccggat gcctcaccgt acgtcaagga ttatgttgaa tggggcgcgg
     3901 ggccgcgcgc atcgcaatat ctgattctcg gcgccaaggc gcgcacgatc ctgcagggcc
     3961 gctatgcggt ttccatcgaa gacatccaag ccctcgcgcc ttccgtgctg cgccatcgca
     4021 tcgtccccaa cttcaaagct caaggcgaag gattgtcctc gagcgatatc atcggccgcc
     4081 tgctcaccga agtaaaaccg tcgggcgacg gcaaagccgg cagataattt tcgttcagga
     4141 accaaggcct tacggaaaaa tcctgccgag agatggctga taattacacg tgggaacagg
     4201 ccgtaggggc gcagcatgct gcgcccaaag tttgggccac cggcggtcgc ctgaccacat
     4261 tttgatgacc tcaacgcagc attgggcaat tctctaaaga tcaggaaagc tcgtcgagca
     4321 gttctctggc ctgcttcaga tcgggtgtgt cgaatccttc ggtaaaccaa ccgtagattc
     4381 ttgtcagcat tcggtgagct tcccgcgtct ttcctgtgct ttgccacata cgagccaatg
     4441 gtatcgccgc ccgcaattcg agcgcgcgcg ctttttgctt ttgggcggta tcgatcgcaa
     4501 gcttaaagca ttctt
//
LOCUS       DASULZ010000555         5652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1078109, whole genome shotgun sequence.
ACCESSION   DASULZ010000555 DASULZ010000000
VERSION     DASULZ010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078109"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1368
                     /locus_tag="VFY96_11375"
     CDS             <1..1368
                     /locus_tag="VFY96_11375"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEX5607110.1"
                     /translation="DIPPVTLPDLKISRPEIYFGEKTTNYVVVGGDTKEFDYAKGQEN
                     VYNTYAGRDGVSLGSFWRRALFAWHYGDIKLLISDNVTGSSRILFRRLIQDRIRRIAP
                     FLVLDHDPYLVVNEGRLVWLQDAYTVSDSLPYSQRVQGGRLNYIRNSVKIALDAYDGN
                     PVFYIADPNDPVLQTYQRIFPALFQPLERMSGSLRSHIRYPEDLFTLQASVYGTYHMQ
                     DPEVFYNKEDLWNFPRESHKGQNATMQPYYTIMRLPGEQREEFILMLPMVPNNRDNMI
                     AWLAARCDGAHYGKVIEFAFSKEKLIYGPAQIEARIDQDTTISQQLSLWNQTGSRVIR
                     GNLLAIPIEDTLLYVEPLYLSAESRQLPELKRLIATSGDRVVMAPDVQSLFAALLAEA
                     PKQTPIVASSTPAGAPRTQASAGTNSEALRHYRQALEALNKGDWRAFGVEMDQLQKSL
                     QGAAP"
     gene            complement(1409..2821)
                     /locus_tag="VFY96_11380"
     CDS             complement(1409..2821)
                     /locus_tag="VFY96_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5607111.1"
                     /translation="MPAAKILVVDDDKNLLELAKTRLAASNYDVTVALGYEEALDAAK
                     VQNFDLAIVDLRLADEDGMTLMEKLHAVYPGIPVIILTGHASVEGAVEAMKRGAYTYL
                     TKPFDPRELVLQIGRALESCKLATENERLRGLLKEKYDFANIVAKSDKMQKVLEAVSR
                     IAKTDSTVYINGESGTGKELIAKAIHLASDRKDESFVAINCAAIPETLLESELFGHEK
                     GAFTGAVKSTKGLFTQANHGTLFLDEIGDMPLSIQVKLLRALEERQFYPVGGEKPVQV
                     DVRVVVATKKDLVEEVKKSSFREDLFYRIHVIPIVLPPLKERLEDIPYLVDHLLKKIC
                     QQMRRDVKGLTPKAMEKLMQHDWPGNVRELENTLEFAVTMTQRDVITDDLVLQTKIPT
                     TPVQTEDPVQAQLLPVNGSFKTLKEARSEFEKAYLVRLLRSCNGKATRAAEIAGKYRA
                     DFYDLLKKHDIKLAEFKRFN"
     gene            complement(2963..3196)
                     /locus_tag="VFY96_11385"
     CDS             complement(2963..3196)
                     /locus_tag="VFY96_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3185 domain-containing protein"
                     /protein_id="HEX5607112.1"
                     /translation="MKSRSVMILGVVLVVLGVLALAYQGITYTKREKILDVGPLQATA
                     DREKTIPLPPLVGGLSLAGGIALLIIGAVKSRP"
     gene            complement(3370..3594)
                     /locus_tag="VFY96_11390"
     CDS             complement(3370..3594)
                     /locus_tag="VFY96_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607113.1"
                     /translation="MKKYLALLVFLSLAQWGCAFLGGAATGALATGAGYEINAKRQMD
                     KLEDDYKNERISRRQYDERKKQIEAGSVIY"
     gene            complement(3768..4985)
                     /locus_tag="VFY96_11395"
     CDS             complement(3768..4985)
                     /locus_tag="VFY96_11395"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5607114.1"
                     /translation="MPQSAVRRDRFRWTILALISISHIIGASAQYGINTLAPFYKDEL
                     QLSRAQVGLFFSAFYLAMTGASFATGWLADRFGVRRTTFNGHLFLGVCIIAAALSPSF
                     EWSCVSFFLAGCGYSFLNPASTIGVMTWFERDERATAMGIKQTGVPAGGVLAAMLAPS
                     LVLIIGWRGALAALGTINFLFGFVFSWLWREPFNATSEQGAEREFPAAGASRLKVWQL
                     LPISCATAIYLVGQMALITYVPLYLKDAMSFSPYWASQALALTQAGAMVGRVGWGVVS
                     DRLFHGRRKIVLIWIGILSALLIAVLGMLQPESSYYVLLPILFLAGVSIVGYQGVSYA
                     LIGEIAGKTRTGAGLGMMIAINSGAATLGTPIFGYIVDRTGSYALAWQMLAVTVSVGI
                     VGLAVLLKEPKAN"
     gene            complement(4997..>5652)
                     /locus_tag="VFY96_11400"
     CDS             complement(4997..>5652)
                     /locus_tag="VFY96_11400"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5607115.1"
                     /translation="IRAIATVEPSANDWTLAEIQEHRRNLDCALRPATGGFAAYHVLD
                     ILREKLPGDGILAYDVGAHTHQIATQWRTDLPHTLLATNGWSSMGYGMPAAYAAKLVY
                     PQRSVVAVIGDGCFQMTAGELSLARRQNLPVPVVVLNDGWLGLMKVKQERRNYRLSGV
                     YLGAPPESPSHYFGVPCRAAKNAEQFDAALDWAVALKGPSVIEAFIDASAYSATVFD"
BASE COUNT         1300 a         1643 c         1483 g         1226 t
ORIGIN      
        1 gatattccgc cggtcacctt gccggatctg aagattagcc gtccagaaat ctatttcggc
       61 gagaagacca cgaattatgt cgtcgttggc ggcgacacca aggaatttga ttacgccaag
      121 ggtcaggaga atgtttacaa cacctacgcc ggacgcgacg gcgtctcact ggggagcttc
      181 tggcggcgcg ctttgttcgc ctggcattac ggcgatatca agctcctgat cagcgacaac
      241 gtgaccggct ccagccggat tctattccgt cgcttgatcc aggatcgcat tcggcgcatc
      301 gcgccttttt tggttctcga ccacgatccc tatttggtgg tgaacgaagg ccgtttggtc
      361 tggctgcagg atgcctacac ggtcagtgat tcgctgcctt attcgcaacg ggttcaaggc
      421 ggacggctca actacatacg caactcggtg aagattgcgc ttgacgctta tgatggcaat
      481 ccggtgttct acatcgccga ccccaacgat ccggttttgc aaacatacca gcgtattttt
      541 ccagcgctct ttcaacccct ggagcgcatg tcgggttcgc tgcgcagcca tattcgttat
      601 ccagaggacc tttttacgct tcaggccagc gtttatggca cctatcacat gcaagacccc
      661 gaggtgttct ataacaagga agatctctgg aattttccgc gcgagagcca taagggccaa
      721 aacgccacga tgcagccgta ctacacgatc atgcgcttac caggggaaca gcgcgaagag
      781 ttcatcctga tgctaccgat ggtgcccaac aatcgcgaca acatgatcgc ctggttggcc
      841 gcacggtgcg atggcgcgca ttacgggaaa gtgatcgagt ttgccttttc aaaagaaaag
      901 ctcatctatg gtccggcaca gatcgaagcg cgtatcgatc aggataccac catctcccag
      961 cagctttcgc tctggaacca gaccggctcg cgagtgattc gcggcaatct tctcgcgatt
     1021 cctatcgaag acacgctgct ctatgtcgag ccgctttact tgagcgctga aagccggcag
     1081 ttgcctgagc ttaagcggct catcgcgact tccggcgacc gcgtcgttat ggcgcccgat
     1141 gtgcaaagtc tcttcgcggc tttgctggca gaagcgccca aacaaacgcc aatcgtcgcc
     1201 tcatcgacgc cggccggagc gccgcggact caggctagcg cagggacaaa ctcggaagcg
     1261 ctgcgacact atcgccaagc actcgaggcg ctaaacaagg gcgattggcg ggcctttggc
     1321 gtggaaatgg accaactaca gaaaagcctg cagggcgccg cgccctaggc caccacgaca
     1381 actgcggctt tgaaaaggat tcagagtttt agttgaaccg cttaaactcg gcgagcttga
     1441 tatcgtgttt tttgagcaaa tcgtaaaaat cggcgcggta ctttcccgcg atttcagcgg
     1501 cccgggtcgc tttcccattg cacgaacgca gcagccgcac cagataggct ttctcgaatt
     1561 ccgatcgcgc ttccttcagc gtcttgaacg atccgttcac cggcaataat tgcgcttgca
     1621 ccggatcctc cgtctgaacc ggagttgtcg gaatcttagt ctgcaaaacg aggtcgtcgg
     1681 tgatgacgtc gcgctgcgtc atcgtgacag cgaactccag cgtgttttcc agctcgcgca
     1741 cgttgcccgg ccaatcatgc tgcatcagct tttccatcgc cttcggcgtc agccctttga
     1801 catctctcct catctgctga caaatctttt taagtaagtg gtctacgagg tacggaatat
     1861 cctcgagccg ttctttcagc ggcgggagga caatcggaat gacgtggatg cgataaaaca
     1921 gatcctcgcg gaaagaactc tttttaactt cctcgaccag atcctttttc gtcgcgacga
     1981 cgaccctgac atccacctgc accggctttt cgccgccgac gggataaaac tgcctctcct
     2041 ctaaagcccg gagcaacttg acctggatgg aaagcggcat gtcgccgatc tcatccagaa
     2101 acagcgttcc gtggttcgcc tgagtaaaaa gacccttggt gcttttaacc gctccggtaa
     2161 acgcgccctt ttcatgaccg aaaagctcgc tctcgagcag agtctctgga atagcagcgc
     2221 agttgattgc gacaaaagat tcgtctttgc ggtcgcttgc gagatggatc gccttcgcga
     2281 tcagctcctt gccggtgccg ctttcgccgt tgatgtaaac ggtggaatcg gtcttggcaa
     2341 tgcgcgatac cgcttccagc actttttgca tcttatcgct tttagcgacg atgttcgcaa
     2401 aatcgtattt ttcttttaac aatccacgta gccgttcgtt ttccgtggca agcttgcagc
     2461 tctcaagcgc ccggccgatc tgcagaacca gctcgcgcgg atcgaaaggt ttggtcaagt
     2521 aggtgtaagc gccccgtttc atcgcttcca ccgccccttc gacgctggca tgcccggtca
     2581 gaatgatcac tggaatgccc ggataaacgg catgtaactt ttccatcaag gtcatcccgt
     2641 cttcgtccgc gagcctgagg tcgacaatcg caagatcgaa attctgcacc ttggcggcgt
     2701 cgagcgcttc ctcatagccg agagccacgg tgacgtcgta attgctcgcg gccaagcggg
     2761 tcttcgctag ctcgagcaga tttttgtcgt catcgacaac caagatttta gcagcaggca
     2821 tatggtcact ctcgtctcga gcttcctcca gacggaaagc tcgactgtgt gggtcgattt
     2881 taagcggtca atctctgtgg gccgaagtca gaccagtcac tgtgtgacat tgcctggcac
     2941 atgtttctgc cctgcagatg tactagggcc gtgatttcac cgctccgata atgagcaaag
     3001 cgatgccgcc cgccagcgat agtcctccca cgagtggcgg aagcgggatg gtcttctcac
     3061 gatcggcggt cgcttgaagc gggccgacat ccaaaatctt ctcgcgttta gtgtaagtga
     3121 tgccctgata agccagcgcc aaaacgccca ggacaaccag aactactccc aggatcatta
     3181 cagatcgact cttcatgatc ggctcctccc ataaatcggg ctccaggtgt tcagttgtct
     3241 tacgccgctg tatcaaggcg ccatggtcgc cgcctaaata cctcaaagcg gtgacacgta
     3301 tgcgctgtca agcaatgttg ctccggtcag tcgatcgcga aattatcccc acactttact
     3361 taaattgact caatatataa cggaaccggc ttcaatctgt ttttttcgct cgtcgtactg
     3421 tcgacgcgag attcgctcgt tcttgtaatc atcttctaac ttgtccatct gacgcttggc
     3481 gttgatctcg tatccggccc cggtcgccag ggcaccggtc gccgcaccac caagaaatgc
     3541 gcatccccac tgggccaagc tcaggaacac taagagagcc aaatactttt tcatgacgat
     3601 acctccgcaa aagaggacac tgatgccctc tatcaatcga tgtgccaagt ggtcgcagca
     3661 taggtaatcg tttgtttttc ggggacacca cgtcctctgt gacacaaagt tttgagcgaa
     3721 ttttacttac atgtaggaaa tttcctatac atcgtttata gcagacttca gtttgctttc
     3781 ggttctttga gcaaaacagc aagaccgaca ataccgacag acacggtcac cgcaagcatc
     3841 tgccaagcga gcgcataaga tcctgtgcga tcgacgatgt agccaaatat cggcgtgccc
     3901 agagtcgcag cacccgaatt aatcgcgatc atcatgccca agccggcgcc ggttcgggtc
     3961 ttaccggcga tttcgccgat aagggcgtaa gaaacgcctt gataaccgac gatcgaaaca
     4021 ccggcaagga agagaatcgg tagcaagaca tagtatgatg actcaggctg cagcatgccg
     4081 agaacagcaa tcagaagcgc ggataatatc ccgatccaga tgagaacaat tttacgccgg
     4141 ccgtgaaaca agcgatcgct taccactccc cagccgacgc gcccgaccat cgcgcccgcc
     4201 tgagttaacg ccagagcctg gctcgcccaa tagggcgaaa agctcattgc gtctttcaag
     4261 taaagcggaa cgtaggtaat caacgccatc tgcccgacca gataaatcgc ggtggcacag
     4321 ctgatcggca gcagttgcca tacctttaac ctactggcgc ccgccgcggg aaattcccgt
     4381 tccgcgcctt gctcgctggt tgcattgaac ggctcgcgcc aaagccagga gaaaacaaac
     4441 ccgaacagaa aattgatcgt gccgagtgca gccaaagcgc cgcgccagcc gataatcagc
     4501 acgagcgaag gcgccagcat cgctgccaag acgccacccg ccgggactcc ggtctgcttg
     4561 attcccatgg cggtcgcccg ctcatcgcgt tcaaaccagg tcatcacgcc aatcgtggat
     4621 gctgggttaa gaaagctata gccacatccg gcgaggaaaa aactgacgca gctccactcg
     4681 aaggaaggcg ataaagcagc ggcaatgatg cagacgccca gaaacagatg cccattgaac
     4741 gttgtcctgc gcacgccgaa ccggtcggca agccagcccg tggcaaatga cgctcccgtc
     4801 atggccagat aaaaagcgga aaaaaacaga cccacctgtg cgcgcgagag ctgcaactca
     4861 tctttataaa agggcgccag tgtgttgatg ccgtattggg cggaggcgcc gatgatatga
     4921 gagatgctga tcagcgcgag gatcgtccag cggaaccgat cgcgccgcac ggctgattga
     4981 ggcatgaata ctggatttag tcgaatacag tcgcggaata ggcgctcgcg tcgatgaacg
     5041 cctcgatgac gctcggacct ttgagggcaa ccgcccagtc gagcgcagca tcgaactgct
     5101 cggcgttttt tgccgcacgg cagggcacac cgaagtagtg cgacggtgat tccggcggcg
     5161 cgccgagata cacgccggaa aggcgataat ttctccgctc ttgtttaact ttcatcagac
     5221 cgagccagcc gtcgttcagt acaaccaccg ggaccggcaa attctgccgg cgcgccaaac
     5281 tgagctcgcc cgcagtcatc tgaaaacagc catcgccgat gaccgcaacc acagagcgct
     5341 gaggataaac cagcttcgct gcgtacgccg caggcatgcc gtaacccatc gacgaccagc
     5401 cattggtggc cagcagcgta tggggcagat cggtgcgcca ttgggtcgca atctgatgtg
     5461 tatgggcgcc gacatcatag gcgagaatcc cgtcgcccgg cagcttttca cggagaatat
     5521 ccaagacatg atatgcggcg aaccctcccg tggctggacg caaggcgcaa tcgaggtttc
     5581 tacgatgctc ctgaatttcg gctaaagtcc agtcgttagc cgagggctca actgtggcga
     5641 ttgcgcgaat cg
//
LOCUS       DASULZ010000556         2230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_267428, whole genome shotgun sequence.
ACCESSION   DASULZ010000556 DASULZ010000000
VERSION     DASULZ010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267428"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..722
                     /locus_tag="VFY96_11405"
     CDS             123..722
                     /locus_tag="VFY96_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX5607116.1"
                     /translation="MVESVRKEFPKLVTLISLLSVVSGCGYNQLQGLDEEVKGAWAEV
                     QNQYQRRADLVPNLVATVKGAAKFEQDTLQQVIQARAAATSVKVDASVVDNPEAFRKF
                     EQAQNQLSGALSRLLVVVEKYPDLKANQNFQSLQAQLEGTENRITVARKRYIERVAEY
                     NKAVRYFPTNLTAKWLLGLQVRPTFAAEESSQKPPEVKF"
     gene            738..1553
                     /locus_tag="VFY96_11410"
     CDS             738..1553
                     /locus_tag="VFY96_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HEX5607117.1"
                     /translation="MIDLLLHDARATRRVIRADPWRAGLLSSFLLLLTASSLARALEV
                     PPLRGRVNDYAGVMSQDQVRTLESQLAQFEQETGHQIAVLTIPTLDGEDIEGFSIRVA
                     ESWKIGKKGFDNGVILLVAVKDRKLRLEVGYGLEGVLPDAIADRIIREYIVPRFRAQD
                     YAGGVIAGIDAVQKVIKQEPLPESARKPNPAPQSDFNSLAMFAIAFVIFALMAFSSGR
                     RSRNQMWSGRGRRYPPIFWGGGGFGGGGFGGGSGGGFSGGGGGFGGGGASGSW"
     gene            1556..2182
                     /locus_tag="VFY96_11415"
     CDS             1556..2182
                     /locus_tag="VFY96_11415"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX5607118.1"
                     /translation="MTADKFFNDQEKERIQQAIVAAESKTAGEIVPIIVTSSARYTEV
                     ELYGLIAGLFVGLVIEWLWSDPWASPFVNLWPIVGACAGFLLGQVPAIKRLVTPKNRV
                     EAAVHTLALASFTQHGLHYTRDHTGILILISLLEHRVEVLADRGINAKVEAATWQEIS
                     KIVADGLKANQACAAVCQAISRCGEILAAHFPRRSDDENELPDRLVTG"
BASE COUNT          484 a          606 c          639 g          501 t
ORIGIN      
        1 atgaactcgg gccttttttt tacgcgccag cttggcttct gcccgttggg caattccgat
       61 aacgcctctt gccctttcat gcctcccgac ttacattcaa acgaaattcg ccggaggaac
      121 taatggtaga gtctgtgcgt aaggaatttc ctaaacttgt cactctaata tcgttgctct
      181 cggttgtctc cggctgcggt tacaaccagc tgcagggtct cgatgaagaa gtaaaaggcg
      241 cgtgggcgga agtgcaaaac cagtatcagc gccgcgcgga tctcgtgccc aatctggtcg
      301 ccaccgtcaa gggggcggca aagttcgagc aagatactct gcagcaggtc attcaagccc
      361 gtgccgcggc aacctcggtg aaagtggacg cgagcgtcgt ggacaatccc gaggcgttca
      421 gaaaattcga acaagcgcag aaccagttgt ccggagccct ttcgcgcttg ctcgtggtcg
      481 tagaaaaata tcctgacctt aaagccaatc agaactttca aagtcttcag gctcagctcg
      541 aaggcactga gaatcggatt acagtagcgc gcaagcgcta tatcgagcgc gttgcggaat
      601 ataataaagc ggtgcgctat tttccgacca atctgactgc caagtggttg ttgggccttc
      661 aagtccgacc aacgtttgct gccgaagagt cctcgcaaaa gccacccgag gtgaagttct
      721 agccggttgg gagatacttg atcgatttgc ttctccacga tgctcgtgcg acacggcgtg
      781 taataagagc cgacccttgg cgcgccggtt tactcagcag tttcctgctt ctattgaccg
      841 catcatcgtt ggcgcgcgct cttgaagtgc cgccgttacg cggccgagtc aacgactacg
      901 ccggagtcat gagccaggat caggtgcgca cgttggaatc gcaactcgcg cagttcgaac
      961 aagaaactgg ccaccaaatc gcggttctga caattcccac actcgatggc gaggacatcg
     1021 aaggtttcag catccgtgtc gcggagagct ggaaaatcgg caaaaaaggt ttcgacaacg
     1081 gcgtcatcct tctagtcgct gtcaaagacc gcaagcttcg gctcgaagtc ggttacggtc
     1141 tggaaggtgt tttgcccgac gccatcgcgg atcgcataat tcgggagtac atcgtgccgc
     1201 gttttcgggc tcaagattac gcgggcggag taatcgccgg cattgacgcc gttcagaaag
     1261 tcatcaaaca agagccgcta ccggaatcgg cgcgcaaacc caatccagct ccgcagtcag
     1321 atttcaattc gttagccatg ttcgccatcg catttgttat ttttgcattg atggcattca
     1381 gctctggccg cagaagccgc aaccaaatgt ggtcgggcag gggccggcgc tatccgccca
     1441 ttttttgggg tggtggcggc ttcggcggcg gtggttttgg cggtggctcc ggcggcggtt
     1501 tcagcggtgg cggtggcggc tttggcggcg gcggcgcttc cggtagttgg taaacatgac
     1561 cgcggacaag ttcttcaacg atcaggaaaa ggagcgtatc cagcaagcga ttgtcgccgc
     1621 cgaatcgaaa accgccggtg agatcgtacc gataatcgtg acgtcctccg cgcgttatac
     1681 ggaggtcgag ctctacggct taattgccgg tctattcgtt ggcctggtta tcgaatggct
     1741 ctggagcgat ccatgggcat cgccgtttgt gaatctctgg ccgattgttg gcgcatgcgc
     1801 gggttttctc ttgggccaag tacccgcgat caagcggctc gtcactccta aaaaccgcgt
     1861 cgaagcggcg gttcacacac tagcgctcgc ttcgtttacg cagcacggtc tccattacac
     1921 tagggatcat accgggatat tgattctgat ttcactgctc gagcatcggg tggaagtcct
     1981 tgccgaccgt ggtatcaacg cgaaagtaga agccgctacc tggcaggaga tctcgaaaat
     2041 agtcgccgat ggattgaagg ccaatcaagc gtgcgcagcc gtgtgccaag cgatcagtcg
     2101 ctgcggcgaa atccttgcag cacattttcc gcggcgctcg gacgacgaaa acgaactgcc
     2161 ggaccgtctg gtcacgggtt agttcagagg gccatgccat gagcccctac gcaaaactac
     2221 tggattctgg
//
LOCUS       DASULZ010000557         3203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1384944, whole genome shotgun sequence.
ACCESSION   DASULZ010000557 DASULZ010000000
VERSION     DASULZ010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384944"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..1151)
                     /locus_tag="VFY96_11420"
     CDS             complement(15..1151)
                     /locus_tag="VFY96_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5607119.1"
                     /translation="MTEHGAFSARMHRALSLTRKEARQIFRDPSSIAIGIVLPVVLIL
                     LFGYGLSLDVTEVPVAVVMEDTSPAATDLAASFQLSHYFAVQPMTSMARAEALMLSRK
                     VDGIIRIRPDFGRRINLGDAEVQVLVHGVDANHARIVQRYAQGAVGQWMARRAAQGYA
                     MATGPIVVQSRMWFNEANESRYFLVPGLIVLIMTLIGALLTALVMAREWERGTLEALF
                     VTPVRTNEILLGKTVPYFALGVIGFVLCLAAAKFLFHVPFRGSIAMLTAASMVYLLVA
                     LGIGLLISCVFRSQFVASQITLLVTFLPAVMLSGFLFDLRSMPALVRLITYVLPARYY
                     VALLQTIFLAGDIWAVILPNMAILSGMAVVLWTFTYRAARKSLA"
     gene            complement(1148..2938)
                     /locus_tag="VFY96_11425"
     CDS             complement(1148..2938)
                     /locus_tag="VFY96_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5607120.1"
                     /translation="MALSGRGIRKTFVRDNREVVLALDDVSLQVNTGTLAALVGPDGA
                     GKTTLIRLAAGVLPFDHGELQVLGINVSADTQQVQDRIGYMPQKFGLYEDLSVQENLD
                     LYADLHGISTKDRHAVYPGLMDMTALGPFTKRLAGQLSGGMKQKLGLACTLVSRPELL
                     LLDEPTVGVDPLSRRELWEIIFRLVHEQSLSVLLSTSYLDEAERCGYVVVMHRGKVLA
                     QGAPGEISQLAAGRSFVADPPPGSKAREFQARLLDAPTIIDAVPVGGQVRFVRATEEF
                     SPTASEGRDEPSTDPLSGAPVSAVAPRFEDGFMVLLHQTGAARESLGAMTVSSAQEQE
                     HHGIAVEVRDLVRQFGAFTAVDHINFEVQRGEIFGLLGPNGAGKTTTFRMLCGLLAAS
                     SGTLRVAGVDLRRARASARQRIGYVAQKFSLYGQLSVAENLEFFASAYNLWGGHKQSR
                     IDWALTEFDLTEFVDLPSGDLPGGFKQRLAMAAALLHEPQLLFLDEPTSGADPLARRE
                     FWRRITALAQQNVTVVVTTHFMEEAEYCDRVAIMDAGRVLAQGTPAEIRSRARSEPKT
                     MPNMEDAFIQIVESARDNEAAHHHSTRGAA"
     gene            complement(2952..>3203)
                     /locus_tag="VFY96_11430"
     CDS             complement(2952..>3203)
                     /locus_tag="VFY96_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013512246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin D"
                     /protein_id="HEX5607121.1"
                     /translation="GVDAFPQRRFEGWIGFISPVAEFTPKSVQTEELRTSLVYEVRVF
                     VKDPADELRLGMPASVFFSTKPTKTEAEKRPIGASAQRE"
BASE COUNT          751 a          958 c          867 g          627 t
ORIGIN      
        1 aggcttccaa catttcaggc caagctcttt cgcgcggcgc gataggtgaa cgtccataac
       61 accactgcca ttccggacag aatagccata ttgggaagaa tcaccgccca gatatcaccg
      121 gctaaaaaaa tagtttggag aagcgcgacg taatagcgcg caggcagaac gtatgtgatc
      181 agccgcacca gtgccggcat gctccgcagg tcaaagagga agccggaaag cattaccgct
      241 gggagaaagg tcacgagtaa agtgatttgg cttgcgacaa attgacttct gaagacgcac
      301 gaaatcagca ggccgatgcc gagagcgacc agcaggtaaa ccatggaagc ggcggtcagc
      361 atcgcgatcg accctcgaaa cggcacatga aagagaaatt ttgccgccgc aagacatagg
      421 acaaaaccaa tgacgccgag cgcaaaataa ggaacggttt tgcccagcag aatttcgttg
      481 gtccgtacgg gagtgacgaa gagcgcttcc aaggtgcccc gctcccattc ccgcgccatc
      541 accagcgccg ttaagagggc cccgattaat gtcatgatta ggacaattaa accgggcaca
      601 agaaaatagc ggctttcgtt cgcctcgttg aaccacatgc ggctttgcac gacaatcggt
      661 ccggttgcca tcgcgtagcc ttgcgcggcg cggcgggcca tccactggcc cacagctcct
      721 tgcgcatagc gttgaacgat gcgcgcgtga ttggcatcga cgccgtgtac cagcacctgt
      781 acttccgcat cgccaagatt gatccgccgt ccaaaatccg gccgtatgcg aatgatcccg
      841 tcgactttgc gtgacaacat taatgcttct gcccgtgcca tagaggtcat gggctgaaca
      901 gcgaaatagt gcgacagctg aaaactggca gctaaatccg tcgctgccgg cgacgtatcc
      961 tccatgacca cggccactgg cacctccgtg acgtccaatg acaagccgta accgaagagg
     1021 aggatcagta cgacgggcaa gacgatcccg atggcaatgc tgcttggatc gcgaaagatt
     1081 tgtcgtgcct ctttgcgggt tagagagagg gcgcggtgca tcctcgccga aaaagcgccg
     1141 tgttctgtca tgccgcccct cgcgttgaat ggtgatgcgc tgcttcgttg tcccgcgccg
     1201 actccacaat ctggataaac gcgtcctcca tgttgggcat tgtcttaggt tcggatcgcg
     1261 ctcggctgcg gatctcggcc ggagttccct gggccaaaac gcgcccggcg tccatgatgg
     1321 caacccgatc gcagtactcg gcttcttcca taaagtgtgt cgtcaccacg accgtgacat
     1381 tttgctgggc gagcgcagta atgcggcgcc aaaattcgcg gcgcgcgagc ggatcggcgc
     1441 cgctggttgg ctcgtcgaga aaaagcaatt gcggctcatg cagcaacgct gccgccatcg
     1501 ccagtcgctg cttaaaacca cccggcaaat ctccgctcgg caaatcgaca aactcggtga
     1561 gatcgaattc agtcagtgcc caatcgatgc ggctttgctt atgaccgccc cagagattgt
     1621 acgcactggc gaagaactcc aggttttctg ccaccgagag ctggccgtaa agtgaaaact
     1681 tctgcgccac atatccgatc cgctggcgcg ccgaagcacg cgctcgacgc aaatcaacgc
     1741 ccgccactcg gagcgttccg ctgctcgccg cgagcaatcc gcaaagcatg cgaaacgtcg
     1801 ttgtctttcc ggcgccgttg gggccgagca aaccaaagat ttctccgcgc tgcacttcaa
     1861 aattgatatg gtcgactgcg gtaaaagcgc caaattgtcg gaccaggtcg cgaacttcca
     1921 cggcaatgcc atgatgctcc tgttcctgag ccgagctcac cgtcatcgcg ccgagcgact
     1981 cacgggccgc gccggtttga tgcagcaaga ccataaaacc gtcctcgaaa cgcggagcca
     2041 cagcactcac gggagctccc gataatggat cggtgcttgg ctcatctcgc ccctcactgg
     2101 cggtcggaga aaattcttca gtcgctcgga cgaatcgaac ctggccgccg actggcactg
     2161 catcgataat cgttggcgca tcgagcagac gtgcttgaaa ttcccgcgct ttactgccgg
     2221 gaggaggatc ggcgacaaag gatcggcctg ccgccaactg gctgatctcg ccaggcgcgc
     2281 cttgggctag aactttccca cgatgcatca ccacgacgta tccacaacgc tcggcctcgt
     2341 ccaagtaaga tgtgctcagc aacacgctta gactttgctc atggaccagc cggaaaataa
     2401 tttcccaaag ctcgcggcgc gataagggat cgactccgac ggtcggttca tccagcaaca
     2461 aaagctccgg ccgagaaacc agcgtacaag cgagaccgag cttctgcttc atgccgcccg
     2521 acaattgccc agccaatcgt ttggtaaacg gcccaagcgc cgtcatgtcc attagacccg
     2581 gatagaccgc gtggcggtct tttgtgctga tgccgtgcaa atcagcgtaa agatcgaggt
     2641 tttcctggac actgagatct tcatacagac caaacttttg cggcatataa ccgattcgat
     2701 cctggacctg ctgggtatcg gcactcacgt tgatacccaa aacttgcagc tcgccgtgat
     2761 cgaatggcaa cacgccagca gcaagacgaa tcagcgtcgt cttgccagcg ccgtccggac
     2821 ccacaagagc cgcaagcgtc ccggtgttta cctgcagcga gacgtcgtcc agcgcaagca
     2881 cgacttcgcg attgtcgcgg acaaaggttt tgcggatgcc acggccgctc agagccatag
     2941 attgacttct attattcacg ttgagcacta gctccgattg gccgcttctc tgcctcggtt
     3001 ttggtcggct ttgtcgagaa aaaaacgctc gctggcatac ccagacgcaa ctcgtctgcg
     3061 ggatctttta cgaacacgcg aacctcataa acaagactgg tgcgaagctc ttcggtctgc
     3121 accgatttag gtgtgaactc tgcgaccggt gaaataaaac cgatccagcc ttcgaagcga
     3181 cgctgcggaa atgcgtcaac tcc
//
LOCUS       DASULZ010000558         2792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1181702, whole genome shotgun sequence.
ACCESSION   DASULZ010000558 DASULZ010000000
VERSION     DASULZ010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181702"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VFY96_11435"
     CDS             complement(<1..281)
                     /locus_tag="VFY96_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HEX5607122.1"
                     /translation="MNPIAVLAIIIFLGGAIAAQAGEADPRSTVLVEGAKKEAKMVFY
                     TSVETEFARALTSAFEAKYPSIKTDIFRSTHEKIFSRLNVERQTGTYTA"
     gene            complement(278..919)
                     /locus_tag="VFY96_11440"
     CDS             complement(278..919)
                     /locus_tag="VFY96_11440"
                     /inference="COORDINATES: protein motif:HMM:NF018627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEX5607123.1"
                     /translation="MQNQPSKTIYVDLDDVLCEAARHFLLIVEREFGKRIEYEQLTTF
                     DVGHACRLRPEEREELYRIVHRPEELSQMAPIPKALEVLRRWENAGFEIGIVTGRPPE
                     SYDASLEWLSKHKISYRSFIMVDKYARFATENTIAISLAELAAQRFCWAVEDSLPMAH
                     YLATTMQVPVALLDSPWNRTDSQQAGVNRYRDWRAIADAAPALIEGVKGGKGP"
     gene            complement(922..1683)
                     /locus_tag="VFY96_11445"
     CDS             complement(922..1683)
                     /locus_tag="VFY96_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607124.1"
                     /translation="MADSQENELEEIVAARLREAGLKFATERAVGGLAPDFVVYAPDG
                     RRFIVETKNWDFPGLTMEASRQAQHYQDAAKADGAFIVIPGLKRNLVSKGVVTVDGLI
                     PALQAEIQSGKTRTNSAEVTKIDKLVFAAMPFAPQYEDVFFVAMSYAAEQVGAVCDRV
                     DRREFQGNVVDEVHRMIRRSSAVIIDLSESKPNVLYEAGYAHALKKPSVHICSTPLEK
                     LPFDVAQWKTTRYEPGQTHKLRRDLTQRLKAIFET"
     gene            complement(1838..2419)
                     /locus_tag="VFY96_11450"
     CDS             complement(1838..2419)
                     /locus_tag="VFY96_11450"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX5607125.1"
                     /translation="MKLPRAFYEQSTIDVAKQLLGKYLVRQHPQGVTVGRIVETEGYV
                     GPQDLACHASKGRTARTEVMFGPAGHAYVYFIYGFYHMLNLVTERQDHPAAVLIRAVE
                     PVTGLDLMKARRRLEKLRDLASGPGKLCQAFAIDRSLNGADLCDGILYIEDHGERQPR
                     LQATPRIGVDYAGKWKDKKYRFFVSDSDFVSRT"
     gene            complement(2501..>2792)
                     /locus_tag="VFY96_11455"
     CDS             complement(2501..>2792)
                     /locus_tag="VFY96_11455"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX5607126.1"
                     /translation="MSGSAFSSGSTIPTASLRFVSGEELLKEWESSPGYHRVFCGRCG
                     SPILKRKDKDPEHLRFRPGTLDCDPGVKASKHIHVGSKAPWVEINDDLPQSR"
BASE COUNT          592 a          801 c          732 g          667 t
ORIGIN      
        1 tccgctgtgt aagtcccggt ttggcgctcg acattcagcc ggctaaaaat tttctcgtgc
       61 gtcgagcgga aaatatcggt tttgatcgac gggtatttgg cttcgaaggc gctggtcaaa
      121 gcacgggcga attcggtttc cacagacgtg taaaacacca tcttggcttc ctttttggcg
      181 ccttcaacga gcacagtcga gcgcgggtct gcctcgccgg cctgagcagc gatcgctccg
      241 ccaagaaaga taattatcgc aagcaccgca atagggttca tggtcctttg ccgcctttca
      301 caccttcaat gagagcgggc gcagcatctg caatcgcccg ccaatctcga tatcgattga
      361 ctccggcttg ttgcgagtcc gtgcgattcc acggcgaatc gagcaaagcc accggtacct
      421 gcattgtggt cgccaagtag tgcgccatag gcagcgaatc ttcgaccgcc cagcaaaaac
      481 gctgtgctgc cagttctgca aggctaatcg cgatggtatt ttccgtagca aagcgcgcat
      541 atttgtcaac catgatgaat gatcgatatg aaattttgtg tttggagagc cattcgagag
      601 atgcgtcgta gctttccggc ggccgcccgg tgacgatgcc gatctcgaac cctgcatttt
      661 cccatcttcg aagcacttca agtgctttcg gaatgggcgc catctgtgac aactcttcgg
      721 gccggtgcac gatccgatag agctcttctc gttcctctgg gcgcagccgg caggcatggc
      781 caacatcgaa ggtggtcaac tgctcatact cgatgcgttt gccgaattca cgttcgacga
      841 tgagcagaaa atggcgcgcc gcttcgcaaa gtacatcgtc aaggtcgaca taaatagttt
      901 ttgacggctg gttttgcacg cctaagtctc gaaaatcgct ttcagtcgct gcgtgagatc
      961 gcggcgcaat ttatgagtct ggccgggctc atagcgcgtg gttttccact gcgccacatc
     1021 gaaaggcagc ttctccagcg gcgtcgagca aatgtggacg gagggtttct tcagggcatg
     1081 agcatatccg gcctcgtaaa gcacgttcgg cttcgattcc gacagatcga taatgaccgc
     1141 gctcgaccga cgaatcattc ggtgcacttc atcgacgaca ttaccctgaa actcacggcg
     1201 gtccactcgg tcacaaaccg cgccgacttg ttcagcggca tagctcattg cgacgaaaaa
     1261 tacatcttca tattggggcg cgaagggcat cgccgcgaac acaagcttat caattttagt
     1321 gacttccgcg gaattggttc gagtttttcc gctttggatc tcggcttgaa gcgccgggat
     1381 cagaccgtcg accgtcacca cgccctttga aaccaggttt ctctttagcc cgggaatcac
     1441 gatgaacgct ccgtcagctt tcgcagcgtc ctgatagtgc tgcgcctgcc ggcttgcttc
     1501 cattgtcaat ccgggaaagt cccagttttt tgtctcaacg atgaaccggc gtccgtccgg
     1561 cgcgtacacg acaaaatcgg gagcaagccc accgaccgcc cgctccgtcg caaatttcag
     1621 ccccgcttcg cgcaagcgcg ccgcgacgat ctcctcaagt tcattttcct gcgaatccgc
     1681 catttagttt atctatttcg ctgctctcgc aaatttaaac ctggatttct cagagctcac
     1741 gacggtgcgc agagcgcaac ttactaacct tcgtgctcgt cgtgtgttcg cggtggaagt
     1801 tcggctgttg tttgccagca acctagggag ttccttttca agttcttgag acgaaatcgc
     1861 tgtcagacac gaagaatcgg tattttttgt ctttccattt accagcatag tccacgccga
     1921 tgcgcggcgt tgcctggagc ctcggctggc gctcgccatg atcttcgata tacaagatac
     1981 catcgcacag atctgcgccg ttcaaagaac gatcgatggc gaaggcctgg caaagttttc
     2041 ccggaccgct ggcgagatcg cgcagtttct cgagtcgacg ccgggctttc atgagatcaa
     2101 gtcccgtcac gggctccacg gctctgatca acactgcggc gggatggtcc tggcgctccg
     2161 ttaccaagtt cagcatgtga taaaacccat aaatgaaata cacgtaagcg tgacctgccg
     2221 gcccaaacat cacttccgtc cgggcggtcc gtcccttcga tgcgtgacag gcgagatctt
     2281 gcggtccgac ataaccctcg gtttccacga tacgcccaac ggtcacccct tggggatgtt
     2341 ggcgcacgag atatttgccc agtagctgct tcgctacgtc aatggtcgat tgctcataaa
     2401 acgcgcgtgg tagcttcaca ccagcgctgg aagggttttt aggtcgggcg atcgggacgg
     2461 gagctctaac cactgaggac tttgacctgt cgattgttct ttatcttgac tgcggcaaat
     2521 cgtcattgat ctccacccac ggggccttcg acccgacatg aatgtgcttc gatgctttca
     2581 cccccggatc gcagtcgaga gttcctggac ggaaacgaag atgctcggga tctttgtcct
     2641 tgcgttttaa gattggcgag ccacaccgcc cgcaaaacac ccgatgatat cccggtgacg
     2701 attcccactc cttcaggagc tcttcgccgg agacaaagcg caacgatgct gttgggatag
     2761 tcgagccact cgaaaatgcc gagccggaca tt
//
LOCUS       DASULZ010000559         2505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_166485, whole genome shotgun sequence.
ACCESSION   DASULZ010000559 DASULZ010000000
VERSION     DASULZ010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166485"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VFY96_11460"
     CDS             complement(<1..393)
                     /locus_tag="VFY96_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607127.1"
                     /translation="MGIIDADTHIDETDDTWEYMNDGESGFKPTTAYPANPDPKLPPA
                     RYWLIDGKRQIRFVRSDKDSGTTVETRELLDVKARLKHMDELGTDIQVIYPTLFLMEA
                     SEKPEINTAMRKSYNRWLGDRCSESGGRL"
     gene            536..808
                     /locus_tag="VFY96_11465"
     CDS             536..808
                     /locus_tag="VFY96_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607128.1"
                     /translation="MVQLVLFSGIVIALTVGVTLFLLVLIRAVRNSTTVTESSQIGPL
                     NADGESPTESHWMLTEDVIRGYDTPELYGEDSIARAGGVTTDKQLR"
     gene            complement(845..2314)
                     /locus_tag="VFY96_11470"
     CDS             complement(845..2314)
                     /locus_tag="VFY96_11470"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX5607129.1"
                     /translation="MKTLLRNALIVTMNENNDVIEHGAIAVDGKRITYVGPAQWTPSS
                     PFDRIIEADRLIAIPGMINAHCHSPANLVRGMMPIRPLEIWRTYYRASLRDMREEDFY
                     ASALLGGMEMLKNGATTVLDHFFGNQAYRFMGAGAAIQAMRDLGLRHVVALTLSDKNY
                     EETIPLDNSVPQAGDEIQRMSASEAKETQAWIDDCEAFIEAFHAPEKLTIACPGPSAV
                     QRCTDRLLVGSAELARRRKLPIHIHLAETKPQAVMGQRIYGTSLLRHLDEIGVLDERM
                     SLAHAIWVEPEDVELFAKRGATPVHNPASNLRIGSGFAPVKEYLRVGVNVGLGTDGAA
                     SNDGQNMFDALRLATLIHNQAGSDFNDWIPPRQAIALATRNGARAFGIDAGVIAPGKL
                     ADIALLRRDTAAFTPLNDVLAQIVFCENGASVHSVIIDGEFVVEAGRLTKVDEAEVLR
                     LATQARLRLDSSIEREFAAAKTMEPSLTEMYFRVFGVKT"
BASE COUNT          542 a          720 c          652 g          591 t
ORIGIN      
        1 gagtcttcct ccggattcgc tgcagcgatc gcccaaccag cggttgtagc tttttcgcat
       61 cgcggtgttg atttcaggct tttcgctggc ctccatgaga aaaagcgtcg gatagatcac
      121 ttgtatgtcg gtgcccagct catccatgtg tttcaagcgc gctttgacgt cgagcagttc
      181 acgcgtttcc actgtcgtcc cggagtcctt atcgctccgt acgaaacgaa tttgtcgttt
      241 tccgtcgatg agccagtaac gcgcgggcgg cagcttggga tctggattcg caggataggc
      301 ggtggtcggc ttgaaccccg actcgccatc attcatgtat tcccacgtgt catcggtctc
      361 gtcgatatgg gtatctgcat cgataattcc cacaatcact catctccttt atcgaaatcg
      421 ctcgcgcggc tggcgaacgt ccgcgcttta cttgtttagt acaaactgaa tgcgcggtca
      481 aaacatgcat agggtcttga cgtgagcttc accgccgcgg tatagcactc gccgtatggt
      541 ccagctcgta ctgttctccg gcatagtgat cgccttaacg gtaggcgtga cacttttttt
      601 gttggtgctg atcagagcgg tcagaaactc tactactgta acggaatcgt cacaaatcgg
      661 cccgctaaac gcagatggcg aaagcccgac agaatcccac tggatgctca ccgaagatgt
      721 gattcgcggt tacgatacgc cggagcttta tggagaggat tctattgcca gggccggtgg
      781 agtcacaacg gataagcagt tgcgctgacc tgggtaaaat gattcaaagg ccgtcttacc
      841 ctcattaagt tttcacaccg aacactcgaa agtacatttc ggtaaggctg ggttccatcg
      901 ttttcgcggc agcgaattct ctttcaatcg aggagtctaa cctcagccgt gcttgcgtcg
      961 ccagtcgcaa cacttctgct tcatcgactt tggtcagccg accggcttcg accacgaact
     1021 caccatcgat gatgacggaa tgcaccgatg cgccgttctc acaaaaaacg atttgagcga
     1081 gaacgtcgtt gagcggagta aatgcggctg tgtcgcgtcg caaaagagcg atgtcggcga
     1141 gcttgccggg agcaattacg ccggcatcga tcccgaatgc ccgcgcccca ttgcgcgtcg
     1201 ccaaggcaat ggcttgtcgc ggcggaatcc aatcgttgaa atcgctgccc gcctgattat
     1261 ggattagcgt cgcaagccga agcgcgtcga acatattctg tccgtcattg gacgctgcgc
     1321 catcagtgcc caaaccgacg ttcactccca cccggaggta ctccttgacc ggggcaaaac
     1381 cgctaccgat gcgtagattg ctcgccggat tgtgcaccgg cgtcgcacct cgtttggcaa
     1441 aaagctcaac gtcctccggc tcaacccata ttgcgtgcgc caatgacatc ctttcatcga
     1501 gaacgccgat ttcatccaaa tgtctcagca aggaagtgcc gtaaattcgc tgtcccataa
     1561 cagcctgcgg cttggtctcc gctaaatgaa tgtggatggg cagctttcgt cggcgcgcca
     1621 gttccgccga gccgacgaga agccgatccg tgcagcgctg cacggccgaa ggccctggac
     1681 aggctatcgt cagtttttcg ggcgcatgaa aggcctcgat gaacgcctcg caatcatcga
     1741 tccatgcctg agtctccttc gcttcgctcg cgctcatgcg ctggatttca tcaccagcct
     1801 gcggaaccga attgtccagc ggtatggtct cttcataatt tttatctgat aaagtcaggg
     1861 ccaccacatg acgtagccct agatcgcgca tcgcttggat cgcggcgccc gcgcccataa
     1921 accggtacgc ctgattgccg aaaaaatgat cgagcaccgt cgtcgcgccg ttcttgagca
     1981 tctccatacc gccgagcagt gcgctcgcat aaaaatcttc ctcgcgcatg tcgcgcagcg
     2041 aagcgcgata ataagtgcgc cagatttcca gcggcctgat aggcatcatg ccgcgcacca
     2101 gattggccgg cgagtgacag tgagcattga tcatgccggg aatcgcaatg agccggtcgg
     2161 cctcaatgat gcggtcgaag gggctggaag gtgtccattg cgccggcccg acgtaggtga
     2221 ttcgcttccc atctaccgca atggcgccgt gctcaatgac gtcattattt tcgttcatgg
     2281 tgacaatcag cgcgttacgg agcaaagttt tcattgatta cctcagctaa aaacaaaggc
     2341 acggagtacc gtgccttggc tcttccgaaa gcgtttgtcg cctagacaaa tcccatcttg
     2401 cggccccagt tctcaggatc ctgaagaaag tcgataatct ggttactaga ctcttcactg
     2461 aaatagcttt cgctcaagcc ggtatccagt acatcatccc aattt
//
LOCUS       DASULZ010000560         5807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_877246, whole genome shotgun sequence.
ACCESSION   DASULZ010000560 DASULZ010000000
VERSION     DASULZ010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877246"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VFY96_11475"
     CDS             complement(<1..372)
                     /locus_tag="VFY96_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607130.1"
                     /translation="MTKFSKLLLLLYILAIGSGASQAVHAQTNFFEGKTIRFIVGFTA
                     GGGYDAYTRTIGRHMGKHVPGNPAIIVENMPGAGSMISANYIYKAAKPDGLTIGHFIG
                     GLFLQQLLAKPGIEFDAAKFNY"
     gene            650..1504
                     /locus_tag="VFY96_11480"
     CDS             650..1504
                     /locus_tag="VFY96_11480"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5607131.1"
                     /translation="MVHSVALSATHMECRNLYDSMTVMTELLAFQPISEKKPAEAMLK
                     HPNSPWVLVLHEAGPDAPVKPMHNHWGVRVRSTEEVDNAYHYLTAHKQQYKLGAIGKP
                     TWSHGSYSCYFVEPGTNGWEIECYEDFARKEAAAQKFGGVKKPHWEEIYPEERFPGRG
                     YVPQAFTHGTLVATDLDANRNFYSDVLNLEVHRFSSHVIYVKHPNTKTYIVCALRENP
                     KVFSPNFRNTLTVASPDHVKELYDEFSQSGKQLGVTEVLPLSEDDSGTSFCFRDPGTN
                     CWEISSQN"
     gene            1692..2720
                     /locus_tag="VFY96_11485"
     CDS             1692..2720
                     /locus_tag="VFY96_11485"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEX5607132.1"
                     /translation="MTEKPLEQMGLRDLRKIMRQKAPAEAWEHFNGAAESKATFRRNP
                     QAFRQYLFRQKIFHEVSEPETSVELFGKTLPIPAITAPVGSFCLIGKDAEREVAEGCD
                     RAGAMMFVSQAAKFGPKEWRAADKSPLVFMAYMNRGKEEVSAYAKLAEDLGFAAVGIT
                     VDTVRPVKIGDEVPLSTKDGKPRRGHKSTVKDIEWMKQQVSLPVVIKGIMGAEDARAA
                     INAGADALVVSNHGGRILDYNRAALEALPEVVDAIGGKTPVLLDSGIRSGGDIVKALA
                     LGARAVLVGRPIAWGVGAFGARGVERVFAILTEEMQRVMTLTGVARLRDVPRSILFRE
                     EWGGLRGL"
     gene            2717..3274
                     /locus_tag="VFY96_11490"
     CDS             2717..3274
                     /locus_tag="VFY96_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004229648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX5607133.1"
                     /translation="MIFVIWAIVFVLGIWLISTYNGLTALRLRLQNAWRQIDVQLKRR
                     YDLIPNLVETVKGYMQYEQDTLQKVIEARSQAMASKGVKEAAEAQNALTQSLNKVFAL
                     MENYPDLKASKNVLELQEELTTTENQIAFARQYYNDLVTQYNTKQQVFPSNILAGLFG
                     FKPGEFFEVAESEKALPRVNLSLSR"
     gene            3284..4294
                     /locus_tag="VFY96_11495"
     CDS             3284..4294
                     /locus_tag="VFY96_11495"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX5607134.1"
                     /translation="MALESPYGSDFAARQAHNQRCSAALVFFFILFFLLIGGAVDLLY
                     FDAFSSTAFPMATIIALSFATISTAAAYYSGSDIILASLGAEKLNLQIPEHRELHNIV
                     TEMALASGSSMPRVYVIFDPAPNALATGTNDRNASICVTSGLLALMDRQETQGVVAHE
                     ISHIRNDDVLLMTLVTILIGGIAILADWSRRGFYSSRSGRRLGSNHVLVLVPLLVLIA
                     FSPLIARLLAMAVSRQREYLADATATQYTRNPRGLAEALEKIRDSGMPFQKATRGSAH
                     LFFANPLRRRIDDRQGKLADLLSSHPPIERRIGLLYQMAGIARLAGASATIQSSTAQT
                     LL"
     gene            4435..4629
                     /locus_tag="VFY96_11500"
     CDS             4435..4629
                     /locus_tag="VFY96_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607135.1"
                     /translation="MDNATIARKLPEAMADPKAWVLTGTRGIYAYPRGEEESDQTVKQ
                     STIGAAKLIVETLVRTILGL"
     gene            complement(4634..5014)
                     /locus_tag="VFY96_11505"
     CDS             complement(4634..5014)
                     /locus_tag="VFY96_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5607136.1"
                     /translation="MEKQIVSTPSAPKAIGPYEQAIKVGDFIYASGQIPLDPATGNLV
                     ENNITIQTRQVMENLKAVLTAAGSSLERVVKTTVFLKNISDFAAMNEVYGHYLNNVKP
                     ARSTVAVADLPRGALVEIDLIAIA"
     gene            5131..5328
                     /locus_tag="VFY96_11510"
     CDS             5131..5328
                     /locus_tag="VFY96_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607137.1"
                     /translation="MRLITAHKILIGTATVFFVFFSLWELRNYAASAEAWALARSILY
                     LMIAIGFGIYLKKLGRLYHLR"
     gene            5346..5621
                     /locus_tag="VFY96_11515"
     CDS             5346..5621
                     /locus_tag="VFY96_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEX5607138.1"
                     /translation="MVPFLIEVSQEQIRRERQKARQLRGSPWWKRKRAAGICHHCGTK
                     CSPKELTMDHLVPIVRGGKSIKGNLVPSCKTCNSERKYRLPFESSEK"
BASE COUNT         1344 a         1527 c         1618 g         1318 t
ORIGIN      
        1 atagttgaat tttgcggcgt cgaactctat gcccggtttg gccaagagtt gctgtaaaaa
       61 taggccgccg atgaaatgac cgatggtcag gccgtcgggt ttcgctgctt tgtaaatata
      121 atttgccgaa atcatgctgc ccgcgccggg catgttttcg acgatgatgg ccggattgcc
      181 cggtacatgt tttcccatat ggcggccgat ggtccgcgtg tatgcgtcgt agccgccgcc
      241 ggcagtgaag ccgacaatga aacgaatggt tttgccctcg aaaaaattgg tttgcgcatg
      301 gaccgcctgc gatgctccac tgccaatagc caatatgtat aagagcaaga ggagtttaga
      361 aaatttcgtc atacggtctc cttctggcga gcataggcgc atctcgggac gttgatcgtg
      421 acagtgctga cgatggcgct ttcgtccggg caaagctgcg cgaatttaag taaaagggct
      481 tgtgctgtca agcgtatttt cgccttgctg cactgcggtg gaatcgcaat tcacgtcatg
      541 attcctcaat gcaatcgctc gctttgcgtc gaaacggctc aatgaccatt gtaagggttg
      601 gtttgacagg tctgcccgag cactgtaaat ttcctgactg gagtcaaaga tggttcactc
      661 tgtcgctctc agcgccacgc acatggaatg ccgcaacttg tacgattcga tgacggtgat
      721 gaccgagttg ctcgcctttc aacccatttc agaaaaaaaa ccggccgaag cgatgctcaa
      781 gcaccccaat tcaccgtggg ttctcgtcct gcatgaggcg ggacccgatg cgccagtgaa
      841 accgatgcac aaccattggg gtgttcgggt gcggagcacc gaagaagtcg acaacgccta
      901 tcactaccta accgcgcata aacagcagta caagctcggc gcgatcggca aaccgacctg
      961 gagccacggt tcctattcat gttatttcgt cgagcccggc accaatggct gggaaatcga
     1021 atgttacgaa gactttgccc ggaaagaagc cgcagcacaa aaattcggcg gcgtcaaaaa
     1081 gccccattgg gaggagattt atcccgaaga gcgctttccg ggacgcggtt acgtgccgca
     1141 ggcttttacc catggaactt tagtggcgac ggatttagat gcgaatagga atttctacag
     1201 cgatgttttg aacttggaag ttcaccgctt cagcagccat gtaatctatg ttaaacatcc
     1261 gaacacgaaa acctacattg tttgcgccct gcgagaaaat ccaaaagtgt tctcacctaa
     1321 tttccgcaat actttgactg tcgcttctcc agaccacgtg aaagaacttt acgacgagtt
     1381 cagccaatcg ggtaagcagt tgggcgtaac cgaggtgttg ccgctcagcg aagacgactc
     1441 tggcacgtca ttttgctttc gcgatcccgg aacgaactgc tgggaaattt cttcccagaa
     1501 ttgagcgcaa cggcaatttt cccggttgtc attctgacag tgaagaatcc ggacagatgc
     1561 acggctgtaa caggcgagat ccgtcgctac gccagaataa tattttttgc gcatctcaac
     1621 agatgggacg ctgcgggaga acggctctcc gacgaatcta gtcgaaagaa aaaacggacg
     1681 gaacaatttt aatgactgaa aagccgctcg aacagatggg cctgcgtgat ctgcgcaaga
     1741 tcatgcggca gaaggcgccg gctgaggcgt gggagcattt caacggcgcg gccgaatcca
     1801 aggcaacatt tcgccgcaat ccgcaggcat ttcggcaata cctctttcga caaaagattt
     1861 tccacgaggt ttccgaaccc gagacttccg tcgagctgtt tggcaagacc ttgccaatcc
     1921 cggcgattac ggcgcctgtc ggaagcttct gccttatcgg gaaagatgcc gagcgcgagg
     1981 ttgccgaggg atgcgatcgc gccggcgcca tgatgttcgt cagtcaggct gcgaagttcg
     2041 gccccaaaga atggcgcgcc gcggacaaat caccgctggt tttcatggct tatatgaacc
     2101 gcggtaaaga ggaagtctcg gcctacgcta aactcgccga agatcttggt ttcgcggcag
     2161 tcggaatcac cgtggatacg gttcgcccgg taaagatcgg cgatgaagtg ccgctctcga
     2221 ctaaagacgg caagccgcgc aggggccaca aatcaacggt taaagacatc gaatggatga
     2281 aacagcaggt ttcgttgccg gtagtgatca aaggcatcat gggggcagag gacgcgcggg
     2341 ccgccatcaa cgccggggcc gatgccctgg ttgtgtccaa tcacggcggc aggattctcg
     2401 attacaatcg cgcggccttg gaagctttgc cggaagtggt tgacgcgatc ggcggaaaaa
     2461 ccccggtgtt gctcgacagc ggaattcgca gcggcggcga catcgtcaaa gctctcgccc
     2521 tcggtgcacg cgccgttctt gtcggtcgtc cgatcgcctg gggtgtcggc gccttcggag
     2581 cgcgcggtgt cgaacgggtg tttgctattt taaccgagga gatgcagcga gtgatgactc
     2641 tgaccggtgt ggcgcgcctc cgggatgtgc cccgatcgat tttgtttcgt gaggaatggg
     2701 gagggttgcg cggcctatga tttttgtcat ctgggcgatc gttttcgtat tgggcatctg
     2761 gctcatttcg acttacaacg gcctcacggc tttgcggctg cgcctgcaaa atgcctggag
     2821 acaaatcgat gtgcaactca agcgccgtta cgatttgatt cccaatcttg tcgagaccgt
     2881 caaaggctat atgcaatacg agcaggacac gttgcaaaag gtgatcgagg cgcggagcca
     2941 ggcaatggct tcaaagggcg tgaaagaggc agccgaggcg cagaacgcgt tgacccagtc
     3001 cctcaacaaa gtcttcgccc taatggaaaa ctatccggat ctcaaggcaa gcaagaatgt
     3061 tttggagctg caggaagagt tgacgaccac agaaaatcag atcgccttcg cgcgtcagta
     3121 ctataatgat ctcgtgacgc agtacaatac gaaacaacaa gtgttcccct cgaatatcct
     3181 ggccggatta ttcggtttca aacccggtga gttcttcgaa gtggcggaga gcgaaaaagc
     3241 actgccccgc gtcaatttgt cgctatcacg gtagcactgg agcttggcgc tggaatcgcc
     3301 gtacggttcg gacttcgccg cgcgccaggc gcacaatcaa cgctgcagcg ctgccttagt
     3361 atttttcttc atcctctttt ttcttctgat cggcggcgcg gtcgacttgc tctatttcga
     3421 cgcgttcagc tcgaccgcct ttccgatggc gacgattata gcgttgagtt tcgcgacgat
     3481 ttcgacggcc gctgcctact acagcggcag tgatattatc ctggcatcgt tgggcgcgga
     3541 aaagctcaat ttgcagattc ccgaacatcg cgagttgcac aacatcgtga ctgaaatggc
     3601 gctcgcgtca gggtcgagca tgcctcgggt ttatgtcatt ttcgatccgg cgccgaacgc
     3661 attggccaca ggaacgaatg accgaaacgc gagtatttgc gtgaccagcg gactgctcgc
     3721 gttgatggat cggcaagaaa cccagggagt ggtcgcgcac gagatctcgc acattcgcaa
     3781 cgatgatgtt ctgctaatga cgctggtgac gattctcatc ggcggcatcg cgatccttgc
     3841 cgactggagc cgtcggggat tttacagctc gcgctcgggt cggcgcctag gaagtaacca
     3901 tgtgctggtg cttgtcccat tgttagtcct gatcgcgttt tcacccctga tcgcgcggct
     3961 tctcgcgatg gctgtctcgc gccagcggga atatttggcc gatgcgacgg cgacacaata
     4021 tacccgcaat ccgcgcggtc tcgctgaagc cctggaaaaa attcgcgatt caggaatgcc
     4081 gtttcaaaag gcaacccgcg gtagtgccca tctcttcttc gccaatccgc tgcgccggcg
     4141 aattgacgat cgacaaggta agctcgcaga cctgctctcg agccatccgc ccatcgagcg
     4201 gcgaattggc ttactgtatc aaatggccgg catagctcgg ttagcggggg caagtgcaac
     4261 gatccaaagc tcaacggctc aaacacttct ctgactcgga aaagtcgctt tgctaagctg
     4321 tccacaatgc gactgtagag atattcaact ctcgcgctgt cgtcattgcg acggtatctg
     4381 gctcaatcgc ggtcagttcc atcactacaa agatttccaa cacgcgcagc caagatggac
     4441 aacgcaacca ttgcgcgcaa gctgccggaa gcgatggccg atcctaaggc atgggtgctc
     4501 accggaacga gagggatata tgcctatccc cggggtgaag aagaaagcga ccaaacagta
     4561 aaacaaagca cgatcggcgc ggccaagctc atcgtagaga ccctcgtgcg gacgattctt
     4621 ggtctctgat cgattacgcg atcgcgatca ggtcgatttc cacaagggcg ccgcgcggta
     4681 ggtcggcaac cgctacggtg gaacgagcag gttttacatt gttgagataa tgtccataga
     4741 cctcgttcat tgcggcaaaa tcggagatgt ttttcaaaaa cacggtggtt ttaacgacgc
     4801 gttccagtga ggagccggca gcggttagga cagccttcag attttccatg acttgacgcg
     4861 tctgaatggt gatgttgttc tcgacgagat tgccggtggc cggatcgagc ggtatttgcc
     4921 cggaggcgta gataaaatca ccgactttga tcgcttgctc gtatgggccg atggctttcg
     4981 gtgcgcttgg ggtgctgaca atttgttttt ccatggaaaa ctctccttca ttcttgatgg
     5041 caacaaagca tatcagcggc cccaactggc gcaacaagtt gcacacggtt gcgcccgaag
     5101 cgccggatgt gtttgattga atcagcggct atgcgactga tcacggccca taagattctc
     5161 atcggcactg cgacagtttt ttttgtgttc ttctcgcttt gggagctacg taattatgcc
     5221 gcgagcgccg aggcgtgggc gttggcacga tcgatcttgt acctgatgat tgcgatcggc
     5281 tttggtatct accttaaaaa actcggccga ctgtatcatc tccgataaga gagctggttg
     5341 tggaaatagt tccattcttg atcgaagtgt ctcaagaaca aattcgccgc gaacgacaaa
     5401 aggcgcgcca attacgcggg agcccctggt ggaagcgcaa acgcgccgca ggcatctgcc
     5461 atcattgtgg aacaaaatgc tcgcccaagg agctgaccat ggaccatttg gtgccaattg
     5521 tgcgcggtgg caaatcaatc aaaggcaatc tcgtgcccag ctgcaaaacc tgcaattcgg
     5581 aacggaaata tcgactgccc tttgaaagca gcgaaaagta gattgcgctt gcgggatttt
     5641 cttcggttca ctaagatcct ttcaaccgga ggtcgccatc atgacagccg aaacattctt
     5701 aattcgcacg atgtccgaca ccgtcgaaac ggatattcgc cgtgttcacg ttatcgccaa
     5761 cgaggtacta gcccggtacg gtatggcacc tctgactttc gagcagc
//
LOCUS       DASULZ010000561         2288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1282877, whole genome shotgun sequence.
ACCESSION   DASULZ010000561 DASULZ010000000
VERSION     DASULZ010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282877"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VFY96_11520"
     CDS             complement(<1..843)
                     /locus_tag="VFY96_11520"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX5607139.1"
                     /translation="MKSTDSLKSHQRLLIFLLLILAITCVISPWMAWGADWLHLNWPS
                     LIDERVPFPRVFNRAFMVAGIIGFVAARKFIFPPRIKPLLLPGLRKATRLLSIGWTLG
                     VASTALLVVFMTVSAIFVPFFRLSWGESISRLISAFFAGIFAGFLEEIFFRGILFLGL
                     RDAGRPWVAYVFANLFYSALHFVKPGEAYFLNGIEPLAGFRHLLSTFEPFNHPLDLLP
                     GIFGLFLIGVILSYALVRTGNLYLSIGLHAGWVFGLKVIRVFGDFTREDLGWIFGSTD
                     PKIVS"
     gene            873..1619
                     /locus_tag="VFY96_11525"
     CDS             873..1619
                     /locus_tag="VFY96_11525"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5607140.1"
                     /translation="MIASSGEQKLRGKVALVTGASRGIGRAIAAAYAREGARVFTCAR
                     HQENLDRAIAEIAAAGGEIAGCTGDVGSAQDVQRIVRTATDRFGIIDVLVNNASILGP
                     RVVIADYPLPAWQEVIDINLTGIFMLTREVLPMMRRQRSGSIINVSSGVGRSGKKRWG
                     AYAVSKAGIECLTQILADELKDSGVRVNAVNPAATRTEMRARAYPQEDPQSLPTPEEV
                     IPIFVDLASDDTVTVSGASLDARNWSRTSH"
     gene            1735..>2288
                     /locus_tag="VFY96_11530"
     CDS             1735..>2288
                     /locus_tag="VFY96_11530"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607141.1"
                     /translation="MMRTIVALCVVFLLSPAALAQELKRIKIGYPAISYNQIHIWVAK
                     EAGLFRKYGLDAEIIFFRGGQTATQALVAGDPPIVNIGTVVQAGLQGHDVVLIASSEN
                     SYNYSVVARPSITKIEQLKGKRLGVSGFGSASHNASLILLKKFNLEPNKDVALVVAGP
                     TTDRLTAVEAGRIDATVLTASELP"
BASE COUNT          607 a          602 c          633 g          446 t
ORIGIN      
        1 gctgacgatt ttcggatcgg tggaaccaaa aatccatcct agatcctcgc gggtaaaatc
       61 tccaaacaca cgaatgactt tgagcccgaa cacccagccc gcgtgcagtc cgatcgacag
      121 atagagattg cctgtgcgca ccagcgcata actcaagatc actccaatga gaaaaagacc
      181 gaaaattccc ggcaagagat caagcggatg attgaacggt tcgaaggtgc tcaagagatg
      241 acgaaatccc gccaacggtt cgatgccgtt taaaaagtag gcttcccccg gtttaacgaa
      301 gtgaagcgcc gaatagaaaa gattggcaaa aacataggcc acccatggac ggccagcatc
      361 acgcaagccg agaaaaagaa ttccgcgaaa aaaaatctct tctagaaagc cggcgaaaat
      421 accggcgaaa aacgcgctga tgagtcgtga gattgactct ccccaagaca gacggaagaa
      481 aggaacgaaa atggcgctaa ccgtcatgaa gaccaccaac agcgcggtgg atgcgacgcc
      541 gagagtccat ccgatcgata acagacgtgt tgctttccgg agaccaggca ggagcaacgg
      601 tttgatccgc ggcggaaaaa taaacttgcg ggcggcaacg aatccgatga tgccagcgac
      661 catgaaggcg cgattgaaga cacggggaaa cggaacgcgc tcatcgatca acgacggcca
      721 gttgagatga agccaatccg cgccccaagc catccacggg ctgatcacac acgtgatcgc
      781 cagtatcagc agcaaaaaaa tgagcaagcg ttgatggctt ttgagtgaat ccgtggattt
      841 catgggcagt ttgactatgt tatagcaaat tcatgatcgc aagcagcggc gaacaaaaac
      901 tgcgcggcaa agtggcactg gtcaccgggg cgagtcgcgg aattggacgc gctattgctg
      961 cagcttacgc gcgtgaagga gcgcgcgtat ttacgtgtgc tcggcatcag gagaatctcg
     1021 accgtgcaat tgccgaaatc gccgcagccg gcggagaaat cgccggctgt acaggtgacg
     1081 tgggctctgc gcaagacgtg caacgcatcg tgcgcaccgc gaccgatcgt ttcggcatta
     1141 tcgatgtgct ggtgaataat gccagcattc tcggaccgcg ggttgttatt gcggattatc
     1201 cgctgccggc ctggcaagaa gtcattgata taaatcttac cggaattttc atgctgaccc
     1261 gtgaggtcct cccgatgatg cggaggcaac gctcgggttc gatcatcaat gtctcgtccg
     1321 gcgtgggccg aagcggaaaa aagcgctggg gtgcttacgc cgtctctaaa gccgggatcg
     1381 aatgccttac gcaaattctc gccgacgaac tgaaagactc cggcgtgaga gtaaacgcgg
     1441 taaaccctgc cgcgacccgg accgaaatga gggcgcgagc ctacccccaa gaagatccgc
     1501 agagcttacc gactccggaa gaagtcatac cgatcttcgt ggacctcgcc tcggacgata
     1561 ccgttacagt gagcggcgcc tccctcgatg cgcgtaattg gagtcgaacg tcgcattgac
     1621 gaaggacgag ccttcatgta caagacgtgg ccatcatgcg atgggtcaaa agattccata
     1681 acaacacgcg cttttaaata attttttgca aagccaaact gggtttaaat ttagatgatg
     1741 aggactattg tcgccctttg cgtggtattc ctattgagtc cggcggcgct cgctcaggag
     1801 ctgaagcgga tcaaaatcgg ctatccggcg atctcctaca atcagattca catttgggtt
     1861 gctaaggaag ccggtttgtt caggaaatac ggcctcgatg ccgagattat tttcttccgc
     1921 ggcggacaga cggcgactca agcgctcgtc gctggcgacc cacccatcgt caacatcggc
     1981 acggtagtgc aagccggctt gcagggccat gacgtcgtcc tcatcgcctc gtcagaaaat
     2041 agttacaatt attcggttgt cgcgcggccg agcataacaa agatcgagca actcaaggga
     2101 aaacggcttg gcgtgagcgg ttttggttcc gcatcccaca acgcgtcact gatcctgcta
     2161 aaaaaattca acctggagcc taacaaagac gttgcgctgg tggttgctgg tcccaccacg
     2221 gatcgtttaa ccgcagttga agcagggcgg attgatgcca cggttctgac ggcctcggag
     2281 ttacccag
//
LOCUS       DASULZ010000562         2344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_674365, whole genome shotgun sequence.
ACCESSION   DASULZ010000562 DASULZ010000000
VERSION     DASULZ010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674365"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            243..1787
                     /locus_tag="VFY96_11535"
     CDS             243..1787
                     /locus_tag="VFY96_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5607142.1"
                     /translation="MRYGFVIDQNRCIGCHACTVACKEEHNIALGVNRTWVKYVEKGT
                     YPDTRRHFAVLRCNHCDDAPCIEICPTVALFRRGDGIVDFDHERCIGCKSCMQACPYD
                     ALYIDPDRNTAAKCNFDASRIEMGYKPACEVVCPTQAILSGDLDDPDSLVSKRIALEK
                     VSVRKPEKGTKPKLFYVGVEGDLLNPTMMEPQTSHFWADKDPGEDLYALKSGNPERPI
                     PGAAREVYDVPHSRPWGNKIAAYLWTKSIAAGLVVLSALFSNMGFQQDAVLLNVISPA
                     LSLLFLVITMALLILDLKKPQRFFFLLTKPNFNSWLTLGGYVLMIFGVFLALWLAQIY
                     LHGRVSPWVVWSAGLFAIASAGYSAFLFAQARGRDFWQSPMLFWHLVIKAVLAGAAVL
                     ILVGALEFVTPYQFVSPQLFLWLINILLISLLATVAIVFGELFMKHGASDMIRAGDLL
                     LKGPFSKLFWLCFVAAGGVLPVALFFLPLGSQIPFYVAAPVLSLFGLWMYDHLWIKAG
                     QALPLS"
     gene            1904..>2344
                     /locus_tag="VFY96_11540"
     CDS             1904..>2344
                     /locus_tag="VFY96_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5607143.1"
                     /translation="MSKQPPPLSRDAQALDASSALASYPPVEKWDDWVEYDTAQWPRK
                     VEKHCHVIPTICFNCEAACGLLGFVEKDTGHIRRFEGNPEHPGSRGRNCAKGPATLNQ
                     VNDPERIRYPLKRVGARGAGKWERVTWDEALDDIAGRVRKALLEE"
BASE COUNT          498 a          598 c          673 g          575 t
ORIGIN      
        1 gaagagttgt tcgctcaagc ccaagctggc gccaatcggg cctgggacaa ttgggcagcg
       61 cgcgattgga acggcagaac cggcgagcag atcaatccgc ctgcatttcc gacgagaaga
      121 aattgaggat tgaaagtctt catctcaatt ctcaacacaa ttgacctcgc gcgaattttc
      181 ctgctatcag actgcaaacg cttgtcgtca agaaaaaact ttgttgatta gaaaggtcat
      241 gaatgcgtta cgggttcgtc atcgatcaga accggtgcat cggctgtcac gcgtgcacgg
      301 tggcttgcaa ggaagaacac aatattgcgc tcggcgtgaa tcgcacatgg gtcaagtacg
      361 ttgaaaaagg cacttatccg gatactcggc gccacttcgc ggttctgcga tgcaatcatt
      421 gcgacgatgc gccttgcata gagatttgtc ccacggttgc gctgtttcgc cgtggcgatg
      481 gcatcgtgga tttcgatcac gagcgttgca tcggctgcaa atcgtgcatg caagcgtgtc
      541 cttacgatgc gctctacatc gatccggatc gtaacaccgc ggccaaatgc aacttcgacg
      601 cgtcgaggat cgagatgggc tacaagccgg catgtgaagt ggtttgcccg acccaagcaa
      661 ttctttccgg cgatctcgac gatcccgaca gcctggtcag taaacgcatt gctctggaaa
      721 aagtcagtgt caggaaaccg gagaagggca ccaagccaaa gctcttctac gtcggcgtcg
      781 aaggcgacct gctcaacccg acgatgatgg agccgcaaac gagccatttc tgggcggaca
      841 aagatccggg cgaagatttg tatgctttga agagtggcaa tccggagcga ccgattcctg
      901 gcgcggcgcg tgaggtttac gatgtgccgc acagccgacc ttggggcaac aaaatcgccg
      961 catatctctg gaccaagtcg attgctgcag gtctggtggt cttgtcagcg ctgttctcga
     1021 atatggggtt tcaacaggac gccgtattgc tcaacgtgat cagccctgcg ctgtcgctgc
     1081 tgtttctcgt gatcaccatg gcgctgttga tcttggattt gaaaaagccc cagcgttttt
     1141 tctttttgtt gaccaagcct aatttcaatt cttggctgac actcggcggc tacgtgctga
     1201 tgatcttcgg agtattcttg gcgctctggt tggcacaaat ttaccttcat ggccgagtct
     1261 cgccgtgggt ggtttggtcg gcggggctct ttgcaatcgc ttcagccggt tattccgcgt
     1321 ttctttttgc gcaggcccga ggtcgcgatt tttggcagag cccgatgcta ttctggcacc
     1381 tggtgatcaa ggcggtgctc gcaggcgccg cggtacttat tttggtcgga gctctcgaat
     1441 tcgtgacccc gtatcaattt gtcagcccgc agctgtttct ctggttgata aatattttat
     1501 taatctcgtt actcgcaact gtggcgatcg tgttcggcga attgttcatg aagcatggcg
     1561 caagcgacat gattcgggct ggtgatctat tgttgaaggg gcctttcagc aaattatttt
     1621 ggctctgctt tgttgccgct ggcggcgtct tacccgtggc gttgtttttc cttccgctcg
     1681 gatcgcaaat tccgttttac gtcgccgctc ctgtcttgtc gttgttcggc ttgtggatgt
     1741 acgaccatct ttggatcaaa gccggacagg cgctgccgtt gagctgacgc agcaggaatc
     1801 agcggctttc aggtatcagg tgttagccga taataagttc aaaaattctc agactgagac
     1861 ctgacagctg acgactgaga gttcctaaag gaagttctgt cttatgagta aacaaccccc
     1921 gcctttaagt cgcgatgcgc aagcactcga cgcttcgagc gctctggcga gctatccgcc
     1981 agtggaaaag tgggatgatt gggtcgaata cgacacggcg caatggccgc ggaaggtgga
     2041 aaagcattgc catgtcattc cgacgatttg tttcaattgc gaagccgcat gcggcctttt
     2101 gggattcgtt gaaaaggaca ccgggcatat ccggcgtttc gagggcaacc cggagcatcc
     2161 cgggagtcgg ggcagaaatt gcgccaaggg accggccacg ctcaatcaag tcaacgatcc
     2221 cgagcggatt cgttatccgc tcaagcgcgt cggcgcgcgc ggcgcaggaa agtgggagcg
     2281 cgtcacgtgg gatgaagctc tcgatgacat tgccggccgc gtacgaaaag cgctgctgga
     2341 agag
//
LOCUS       DASULZ010000563         5508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1385712, whole genome shotgun sequence.
ACCESSION   DASULZ010000563 DASULZ010000000
VERSION     DASULZ010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385712"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="VFY96_11545"
     CDS             complement(<1..838)
                     /locus_tag="VFY96_11545"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5607144.1"
                     /translation="MKTMIQGGWVIGFDGQAHEVIKDGVVVFEGNRIEFVGADYSAPV
                     DKKIDAKGCLVSPGFIDTHFHSGINASDYILNDSSKTDFFASNYLAHGGPTRAGAHAA
                     HLKDVDIGQRFSLIHLLKSGVTTTFDIGGAGSEPERYVDMIDQVGMRCAAGPSYKNVS
                     FFHDHEGRLEYDWDDERGSQGLKAAIAFAKKFNGACSGRVSAQLFPGHVDSCTPALLQ
                     ETRKAAQELGVRVQIHATINLFEFHTVMQRERCTPIELLQKIGFLDPEVSLSHCIFIS
                     GHS"
     gene            complement(876..1829)
                     /locus_tag="VFY96_11550"
     CDS             complement(876..1829)
                     /locus_tag="VFY96_11550"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607145.1"
                     /translation="MADANPYLAKAGERPLHVRIATCAVSGGFAHLYTALENRLFEPY
                     GFKLEHVFIRGSSASLAALMADEIQFLYCAADATIPAMVAGADAKLVAAPLLKLPYVL
                     VTRKEIHRVEELKGKSLGVARAGDLSDRLSRMLVKKLNVADVTMRPIGGSQTERYQAM
                     AANIVQGVVITPPLDVRAKNDGYNVAYRLIDLDIPFIYSSLHTSSGMIREKPETVQRM
                     VAAFAAAVNFTEHNPGKAKAAISKIMHIKDEEALQVAYNVYTKDIVDRHMVVPAAAVA
                     ESVERQRSLGTPIKKRVEDLYDNSFVLQLEKSGFLKELWSK"
     gene            complement(1990..3024)
                     /locus_tag="VFY96_11555"
     CDS             complement(1990..3024)
                     /locus_tag="VFY96_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HEX5607146.1"
                     /translation="MKVGLIGHRGAGKTTIFNMLTGLQAQVGGFAGKEEIHLGVIKVP
                     DPRIDRLSQIFKPKKTTYAEIRFTDFPPNEGEENLKSNQALVTQMREVDAITLVLRAF
                     DSAANPLRELTDLLTEMILADLTIVENRRSRLKKEKARPQEEALLERCAQGLESEQSL
                     RTLQFTADEENLASGFGFLSRKPLLVIFNQAEEQAGRPLEEDYQAELDRRGLQGLALA
                     GKVEMEIAELDEKDRAVFLKEIGIEEPARDRFIRAAYGLMDFLSFFTTGEDEVRAWTI
                     TKGTVAKKAAGKIHSDIERGFIRAEVVSYDDFILYGSEAKCREAGKLRLEGKDYLVKD
                     GDIIHFRFAV"
     gene            complement(3178..4524)
                     /locus_tag="VFY96_11560"
     CDS             complement(3178..4524)
                     /locus_tag="VFY96_11560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEX5607147.1"
                     /translation="MAALSNAQAPAVSQGLPDFATLAKRVGPSVVNVSTTQVRKAAAD
                     VPSPFDGDDPMSQFWQRFFGGRIPRGPQRQMGLGSGFIIDHNGTILTNYHVVDGAQKI
                     SVTLSDGKSYDAKVIGKDQKTDIAVIKIDAGQDLPAVMLGDSDRLEVGEWVMAIGNPF
                     GLDHTVTSGIVSAKGRQIGAGPYDNFIQTDASINPGNSGGPLLNLRGEVVGITTAIYS
                     QSGGNIGIGFAIPTNSIKELLPQLKDKGRVVRGYLGTTVQKITPEIADSLGVKQQRGA
                     LVADVVRGSPAERAGIKTGDIITEFNSKEVKDSAELPGLVARVEPGTTANIKVLRDGR
                     EMSLPITIGEMKDTEVAAATQEGDLGLAVQPVTPEIAQSLGLNRAEGLVITEIKPGSA
                     ADEAGLREGDLITQINRRPVKNLADYNREMARSKKGQSVLLLVRRGDSSVFLALKR"
     gene            complement(4729..5184)
                     /locus_tag="VFY96_11565"
     CDS             complement(4729..5184)
                     /locus_tag="VFY96_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607148.1"
                     /translation="MIAKKAVKGRQNHQRGRSPFWLNGVGQPRAPMEERALAVFQPDI
                     LVENQYQSTYRRRFHLEPERVLMLAVLQDAVVCFQDNVTATCKRKRLMHLDAEQWIVN
                     DDRNYPFSFENVCEALGYDPAYMRQGLMRWKDAVLSSRGKKESRKLSVG"
BASE COUNT         1171 a         1609 c         1400 g         1328 t
ORIGIN      
        1 agctgtggcc actgataaaa atgcaatgtg agagagaaac ctccggatcg agaaagccga
       61 tcttctgcag cagctcaatc ggcgtacagc gctcgcgctg catcaccgtg tgaaattcaa
      121 aaagattgat ggtggcatga atctgtacgc gcacaccgag ttcttgcgcc gctttgcgcg
      181 tctcttgcaa aagcgccggc gtacaggaat ccacgtgccc gggaaaaagc tgcgcgctca
      241 cgcgcccgct gcaggcgccg ttgaattttt tggcaaacgc gatggcggct ttgagtcctt
      301 gactgccgcg ctcgtcatcc caatcgtact ccaaacgccc ctcgtgatcg tgaaaaaaac
      361 tcacgttctt gtagcttggc cccgccgcac agcgcatccc gacttggtcg atcatatcga
      421 catagcgctc gggctcgctc cccgcgccgc caatgtcgaa cgtcgtcgtg acgccgctct
      481 tcaagagatg gatcagtgaa aaacgctggc cgatgtcgac gtcttttaaa tgggccgcat
      541 gcgcccccgc gcgggtcggg ccgccgtgcg ccaagtagtt ggaggcaaaa aaatcggttt
      601 tggacgaatc attgagaata tagtcgctgg cgttgatgcc agagtgaaaa tgcgtgtcga
      661 taaatcccgg agagaccaaa catcctttgg catcgatttt cttgtccacc ggagctgagt
      721 aatcggcacc gacgaattcg atgcgatttc cttcgaaaac caccacgccg tccttgatga
      781 cctcatgcgc ttgaccatcg aagccgataa cccaaccgcc ttggatcatt gttttcattg
      841 cgcgcctttc agatgtgtcc gatttcgccg aagcttcact tactccagag ttcctttaga
      901 aaaccgctct tttccaattg caggacaaat gaattgtcgt aaagatcctc cactcttttt
      961 ttgatcggcg taccgagact acgttgccgt tcgaccgatt cggccactgc cgccgcgggc
     1021 acgaccatgt ggcgatccac gatatccttc gtatagacgt tgtatgcaac ctgtaaagct
     1081 tcttcgtctt tgatatgcat gattttgctg atcgccgcct tcgcttttcc ggggttgtgt
     1141 tccgtaaagt taacggccgc ggcaaacgcg gcaaccatgc gctgcacggt ttccggtttt
     1201 tcgcgaatca ttcctgagct cgtgtgaagc gagctgtata taaacgggat atcgagatcg
     1261 atcaagcgat aggcaacatt gtagccgtcg tttttggcac gcacgtcgag gggcggcgtg
     1321 atcaccacgc cttggacaat attggcggcc atggcctgat accgttcggt ctggctgccc
     1381 ccgatgggcc gcatcgtcac gtcggcaaca ttgagctttt tcaccaacat tcgcgatagc
     1441 cgatcactca aatcccccgc ccgagcaacg cccaacgatt tgccttttag ctcttcaact
     1501 cggtgaattt ctttacgggt aacgagaacg tacggcagct tcagcaatgg cgcggcaacg
     1561 agcttggcgt ccgcgccggc gaccatcgct ggaatcgtcg catctgcggc gcaatagaga
     1621 aactgaattt catccgccat cagcgcagcc agcgatgcac tgctcccacg aatgaagaca
     1681 tgttcgagtt taaagccgta tggctcaaat agccggtttt cgagcgccgt atagagatgt
     1741 gcaaaaccgc cggataccgc gcaggttgcg atgcgcacat ggagcgggcg ctcgccggct
     1801 ttggcgagat agggatttgc gtctgccaac gaaaaagatg tgacgatggc taggattatc
     1861 gatgctgaaa aacccgctag tctccgcata ctcgttagaa gcacagccac tgttgatctg
     1921 ccatcaattt ttggcgcggc ggatcatatg ctatttcaaa ggcctactca aatcagcgca
     1981 cccgatcgtt caaaccgcaa aacgaaagtg gataatgtcg ccgtccttca ccaggtagtc
     2041 ctttccctcg agcctgagtt tccccgcttc tctgcattta gcttcgctgc catacagaat
     2101 aaaatcgtcg taacttacga cctcggctcg aataaagccg cgttcgatgt cgctgtgaat
     2161 ttttccggcg gcttttttgg ccacggtccc tttcgtaatc gtccaagcgc gcacttcgtc
     2221 ttcaccggtt gtgaaaaaac tcaagaaatc catgagccca tacgccgcgc gaatgaatcg
     2281 atcacgcgcc ggctcttcga tgccgatttc cttgagaaat acggcgcggt ctttctcatc
     2341 cagttcggcg atctccattt cgactttgcc ggcgagcgcc agcccctgaa gaccgcggcg
     2401 atcgagctct gcttggtaat cctcctcgag cgggcgcccg gcttgctctt ccgcttgatt
     2461 gaaaatcact agcaacggtt tgcggctcaa aaacccgaac cctgaagcga gattttcctc
     2521 atccgcagtg aactggagag ttctgagact ttgctcgctt tccaggcctt gtgcgcagcg
     2581 ttcgagcaag gcctcttcct gcgggcgcgc cttttctttt ttcagccggc tgcgtcgatt
     2641 ctcgacgatc gtgaggtcag caagaatcat ttcggtcagt aagtcggtga gctctcttag
     2701 cggattagcg gcgctgtcga aagctcgcaa gaccagcgtg atcgcgtcga cctcgcgcat
     2761 ttgcgtgacg agggcctgat tggattttag attttcctct ccttcgttgg gtggaaaatc
     2821 ggtgaaccgg atctcggcgt aggtggtctt ctttggcttg aagatctgtg acaggcgatc
     2881 gattcgagga tccgggactt tgatgacgcc gagatgaatt tcttcttttc ccgcaaagcc
     2941 gccgacctga gcctgcaacc cggttagcat gttgaagatg gtagtttttc cggcgccccg
     3001 gtggccgatc agaccgactt tcatttatgt tcctcgtctt cgagagaggc aatctagcag
     3061 gaaacccgcg gtgaataaat gtgccttgat tagctcagat atttccaaaa aaatgcccag
     3121 ctcccgtgcg ttgacttgca acgccagggc tgggcataag cagcaaaaaa ctggtgatta
     3181 tcgcttcagc gcgagaaata cactcgagtc acctcgtcgc accaggagta aaaccgactg
     3241 gcctttcttg ctccgcgcca tctcccgatt gtaatccgcg agattcttga ccggccgacg
     3301 gttaatttgc gtgatcaagt cgccttcgcg caacccggct tcgtcggctg cgctcccggg
     3361 tttgatttca gtaatcacca gcccttccgc ccgatttaat cccaggctct gggcgatttc
     3421 cggggtcacg ggttgcactg ccaatcccaa atcaccttct tgcgtagcgg cagcaacttc
     3481 ggtgtccttc atttccccaa tcgtaattgg gagactcatt tccctaccat ctcgcagcac
     3541 cttaatgttc gcggtggttc caggttcgac acgcgccacc aaacccggta gctccgcgga
     3601 gtctttaacc tctttggaat tgaactcggt aatgatgtcg ccggtcttga ttcctgctcg
     3661 ttccgccggg ctgccccgca cgacatccgc caccagcgct cctctctgct gttttacacc
     3721 gagagaatca gcgatctccg gagtgatttt ttgcaccgtc gtaccgagat aaccacgtac
     3781 gacccggcct ttgtctttga gctgcggcaa aagttccttg atcgaattgg tcggaatggc
     3841 aaagccgatt ccaatgtttc cacccgactg gctgtaaatc gccgtggtga tgccgacaac
     3901 ctcgccgcgt aggttgagta acggaccgcc cgagttcccc ggattgatgg acgcatcggt
     3961 ctggataaaa ttgtcgtagg gacccgcgcc aatctgccgg cccttggcac tcacgatccc
     4021 tgaagtcacc gtatgatcca aaccaaacgg attgccaatt gccatcaccc attcaccgac
     4081 ttcgagccga tcggaatcgc cgagcatcac ggcgggcaag tcttgccccg catcgatttt
     4141 gatcacggcg atatccgtct tttgatcctt gccgatcact tttgcgtcgt agctctttcc
     4201 gtccgataag gtcacggaaa ttttctgcgc gccatcgacc acatgatagt tggtcaggat
     4261 cgtgccgtta tgatctataa tgaagcccga tcctagcccc atttgccgct gcggtccgcg
     4321 tggaattcgg cctccgaaaa accgctgcca aaattggctc atcggatcat cgccatcgaa
     4381 tggcgagggc acgtccgccg ctgctttgcg cacctgagtg gttgagacgt tcactaccga
     4441 tggacccact ctcttggcca gggtcgcgaa atccggcaac ccttgagaca cagccggagc
     4501 ctgagcgttg ctgagcgccg ccatccggcc gccagcctga tactgatcga ttgcgctgct
     4561 gatacttacg cccgcaagca ggcataatac actgccgaaa actagcgatg aaaccctaat
     4621 gcttttttgt tttgttagcc tactgaacat ctttgagcct ccgctcgatt agacgcggcg
     4681 tcgtccactt cggttcacat ctaagcccgc cggcgtcatc caaccgaatt aaccgactga
     4741 taatttcctg ctctccttct tgcctcgact gctaagcact gcgtctttcc agcgcatgag
     4801 tccttgcctc atgtatgccg gatcgtagcc cagcgcttcg caaacgttct caaaagagaa
     4861 tggataattg cgatcgtcat tgacgatcca ttgttccgca tctaaatgca tcaagcgctt
     4921 gcgcttgcat gtcgctgtca cgttatcttg aaaacaaact acggcatcct gcagaaccgc
     4981 gagcatcagc acgcgctccg gttccagatg aaagcgccga cggtatgtcg actgatattg
     5041 gttttccacc agtatgtccg gctgaaaaac ggccaatgcc cgttcttcca taggcgcccg
     5101 cggctgcccg actccattaa gccaaaacgg cgaccggccc cgctgatggt tttggcgccc
     5161 tttcactgct tttttggcga tcatgactac ctccaattgc ttagacagcg gtttggcttg
     5221 gatggttcca atttttccgc gtcaagcgtt acatgaatga cggaatcccc agaggcaccc
     5281 tatgggcgcc aaagggcgac ataaatcaac gcctgaactg gctggcagtc ttagatcagc
     5341 aggacagaat cgagcaggtg aagggaaatc ttcgacgtat agggattggc gtagatgagc
     5401 ccaccatcgc gggtgctgcg aggcaggctc tctggcctgc tggtttgaac cccacccatg
     5461 cccagcgccg gtgcatcgca ggtttcgcaa tccagaccgg cccgcagc
//
LOCUS       DASULZ010000564         3313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_674686, whole genome shotgun sequence.
ACCESSION   DASULZ010000564 DASULZ010000000
VERSION     DASULZ010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674686"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VFY96_11570"
     CDS             complement(<1..724)
                     /locus_tag="VFY96_11570"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607149.1"
                     /translation="MRRIFRWLIFFALCMSPLASSPLGAATIAEVVAKIKPLNPQERL
                     NYLVQGARGEGELVYYGTLPIDEFLPLARVFNGRYRSLALQHYFSPRDGILSRTLTEA
                     RAGRHAVDVVQVDLSYGYQLLNANLVQPYAVAGANQFYEGTYDPSGFWHSMYYLTTAL
                     IYNSNSIKAEQAPKTYDDLLDPAWKEKMVFDPEAGYILAALEQAWGREKALAYLSKLS
                     KQNLTYRRGGTLTTQVVSSGEFP"
     gene            complement(906..1874)
                     /locus_tag="VFY96_11575"
     CDS             complement(906..1874)
                     /locus_tag="VFY96_11575"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX5607150.1"
                     /translation="MARMNARMMRTKMALEVAAELYTRRMFSMGNVATISIQPSSREE
                     QFPGVIMGINGDRMGGMKAPIEVAKRRVDVAFINPSAIVTMAYRGKGFYKERLALRAL
                     AAFPSWDKMAFAVAKELKLKSLFEIRERKLPLRVSTRPSGIDNTTHYSVTQILSLYGF
                     SFAKIKQWGGTVQETPRPTSPERLSGIRTGKINAIFDEGLHNWLDEALEQGFEVLPIE
                     PKILKQLSSLGYQPSVIPSTKFKQLKGDVETIDFSGWPLITHAGFSDDLAYAICEAID
                     ARQGIIPIDDESPLDMKKIARATEAAPLGIPLHPGAKRYYREKGYL"
     gene            complement(1926..3005)
                     /locus_tag="VFY96_11580"
     CDS             complement(1926..3005)
                     /locus_tag="VFY96_11580"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5607151.1"
                     /translation="MAIFSGEVGLQFTTHLASRYSVPQWVELAALAQRHNFAQLWVND
                     NLGHRNIFVLLTAIASKVPIKLGTAILVPYFRNPIDTADALATLSECMEGHEISVGIA
                     RGDYAQAGNQLHMRKPIAMVKETVECVKSLLHGDAVVYGDYPVLAEFFNLRTDAKIRL
                     GFAPKSPIRFYCGGNGPRIMEIAGRIMDGVLIGGFFIPLVRSGRLSGLLEQAELGRKN
                     AAGAARLRKVCEINISVAHEASAARNFPKRYIAHMLVVLEAMGFSEEEFEALGVDRQR
                     VLQIKKAFEAGGTIEQVSALITDAMVDSGFIAGTPSQCIERLEEMCGYAQEYGFDQIC
                     LSKLGPHYDEAITVLSRDLLPSLAR"
     gene            complement(3085..>3313)
                     /locus_tag="VFY96_11585"
     CDS             complement(3085..>3313)
                     /locus_tag="VFY96_11585"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HEX5607152.1"
                     /translation="RVKISGVKHIVEAKLSDDVVSTHLHIPQGSPILYVERDHFHKKR
                     LIMRTAGFYRSDFFRYELKLKRKRIDRRVER"
BASE COUNT          769 a          936 c          849 g          759 t
ORIGIN      
        1 tgggaaattc acccgagctc acaacttgcg tcgtcaaagt gccgccgcga cggtaggtta
       61 aattctgctt ggacaacttg ctcagatacg caagcgcttt ttctctgccc cacgcctgtt
      121 ccaatgcggc aagaatatag ccggcctcgg gatcgaagac cattttttct ttccatgcgg
      181 gatccagcaa atcgtcgtag gttttcggcg cttgttccgc cttgatggag ttggagttgt
      241 aaatcagcgc cgtggtcaag tagtacatgc tgtgccagaa tccgctggga tcgtaggtgc
      301 cttcataaaa ctgattggcg ccggcgactg cgtatggctg caccagatta gcgttcaaca
      361 actgataacc gtaactcaga tccacctgaa cgacatcgac ggcgtggcgt cccgcgcgtg
      421 cttccgtcaa tgtgcggctg agaatgccgt cacggggcga gaagtaatgt tgcagcgcca
      481 gggagcgata gcgcccgttg aaaactctag ccagcggaag aaattcgtcg ataggaagcg
      541 tgccgtaata aaccaactcg ccttcgccgc gagcgccttg caccagatag ttcagtctct
      601 cttgtggatt gagtggcttt attttcgcca ccacttccgc aatcgtcgca gccccgagag
      661 gcgaggatgc caacggcgac atgcacagtg caaaaaatat cagccagcgg aatattctgc
      721 gcatcaaagc atgctcactt tcatgggcgg tgaagaaatg gccaggtttg ttagatcaaa
      781 caaccgcgcg accgtcaaac cgctggttgg agtggcacga tgaaacatcg ttaatcgccg
      841 aatcgcagaa acctgcgacc gacagaagaa aatatgcaca aaaatactgc gatggtccga
      901 gggacttaca gatagccctt ctctcggtaa tacctttttg cgccgggatg tagaggaatc
      961 ccgagaggcg cggcctctgt tgcacgcgcg attttcttca tatcgagggg gctctcatca
     1021 tcaattggaa taattccctg gcgcgcgtcg atggcctcgc aaatcgcata ggcaagatca
     1081 tcgctgaagc cggcatgagt aatcagcggc caaccgctga aatcaatggt ttcgacgtcg
     1141 cctttgagct gcttgaactt cgtagacgga atgaccgacg gctgataacc gagactcgat
     1201 aattgtttca aaatttttgg ctcgatgggc aaaacctcga atccctgttc gagggcttcg
     1261 tccagccagt tatgcagccc ctcatcgaat atcgcattga tcttacccgt tcttattccg
     1321 gacaaacgct ccggagatgt gggccgcggc gtttcctgaa ccgttccgcc ccattgcttg
     1381 atcttggcga acgagaatcc gtaaagcgat agaatctgcg tcaccgagta gtgagtcgta
     1441 ttgtcgattc ccgaaggccg agtcgacact ctaagcggca gcttccgctc gcgaatttca
     1501 aataacgatt tgagcttcaa ctccttggcc acggcaaaag ccattttgtc ccaggatgga
     1561 aaagcggcca gtgcgcgcag cgccagccgc tccttataaa agcctttgcc tcgataggcc
     1621 atcgtgacaa ttgccgatgg attgatgaag gcgacgtcaa ctcgccgttt cgccacctcg
     1681 atcggcgctt tcatgccgcc catccgatcg ccgttgatac ccataatgac tccgggaaac
     1741 tgctcttcgc gtgaactcgg ctgaatcgag atcgtcgcca cattgcccat gctgaacatg
     1801 cgtcgcgtgt ataattccgc cgcaacttcg agcgccattt ttgtccgcat catgcgcgca
     1861 ttcattctag ccataagcat tccccgtctc gaacatcaga atcattggag ccgaggcaaa
     1921 ttcgtttatc gtgcaagcga gggcaataga tcgcgggaga gaaccgtaat cgcctcgtcg
     1981 tagtgcgggc cgagcttgga caagcagatt tgatcgaaac catactcttg cgcgtagccg
     2041 cacatttctt cgagtcgttc gatgcactgg ctcggtgtgc cggcgataaa gccgctatcg
     2101 accatggcat ccgtgatgag agctgatacc tgctcgatgg tgccgcctgc ttcgaaggct
     2161 ttcttgattt gaagcactcg ctgtcgatcc acccccaatg cctcgaactc ttcctcggag
     2221 aagcccatcg cctcgagcac gaccagcata tgcgcaatat atcgcttggg aaaatttctc
     2281 gccgcgctag cttcatgggc gacggaaatg ttgatttcgc aaaccttccg cagacgtgcc
     2341 gcgccagccg catttttcct tccaagctcg gcctgctcga gcaggccgga tagccttccg
     2401 ctgcgtacga gagggatgaa gaatccgccg atcagaacgc cgtccataat tcgtccggca
     2461 atttccatga tccgcggacc gttaccaccg caataaaatc gaatcggcga tttcggcgcg
     2521 aagcccaatc gaattttggc atccgtgcgc agattgaaga actcggcgag aacaggatag
     2581 tcgccgtaca caaccgcgtc gccgtgcaaa aggctcttca cgcattcgac tgtctctttc
     2641 accatcgcga tcggcttgcg catgtggagc tgatttcccg cttgcgcata atcgccacgg
     2701 gcgatcccca cgctgatctc atgcccttcc atgcattccg acagtgttgc cagcgcatcc
     2761 gcggtatcga ttggatttct gaaataaggc acgaggatcg cggtgccgag cttgatcggc
     2821 accttcgaag cgatcgctgt caaaagcaca aaaatatttc tgtgcccgag attatcgttt
     2881 acccaaagct gagcgaaatt atggcgttga gccagggctg cgagctcgac ccattggggg
     2941 accgaatagc gactggcaag atgcgtcgta aactgcagac ccacttcacc gctgaaaata
     3001 gccacggcag cctcccttct ccacgatcac tgccggcact cgcgagaacg cattggatcg
     3061 cattgtctgc caatcagcca gagcttatct ctcaacacgc cgatctatcc tctttcgttt
     3121 caatttcagc tcgtagcgaa aaaaatcgct tcggtaaaaa cctgcggtcc gcatgatcag
     3181 gcgtttttta tgaaaatggt cgcgctcgac ataaagaatc ggcgagccct gcggaatgtg
     3241 caggtgcgta gacacgacat cgtcggaaag tttcgcttca acgatgtgtt tcacaccgct
     3301 gatcttaacg cgc
//
LOCUS       DASULZ010000565         7097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_471743, whole genome shotgun sequence.
ACCESSION   DASULZ010000565 DASULZ010000000
VERSION     DASULZ010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7097
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471743"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1260
                     /locus_tag="VFY96_11590"
     CDS             <1..1260
                     /locus_tag="VFY96_11590"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX5607153.1"
                     /translation="QRQIRQMGGSAECCVLGTSVLAPPAKAALANGVAGHAMDYDDTQ
                     LSTSKEAVYGLLTHPTTPVLAAVLALGQKRKITGAEFLLAYVLGVEVECRIADAIDPH
                     HYQSGFHSTATMGGLGAAMAAGKILRLKETQLLATLGIAASMASGLRENFGTMTKPLH
                     AGRAAENGVTAALLARDGFTAAANILEARRGFFNAMAGGYDDSKISGRLGSPYFMKEP
                     GISIKPYPSGSLSHPAQDLILDLVKHHDVKAKDIERIDVGTNSNVPNALIYPMPKTAL
                     EGKFSIPFCMAIGVLERKAGIAQFQDRKVRDKNVVDLIKRVTLYVDEELERLGYDQVR
                     SRIRIKLRDGRIIEGRYDVARGHPTKPMSWSELTEKFRDCAGLVLAKKNIEETIRLVE
                     GFENLKSLRPLVRALTNGQEKRTMKHK"
     gene            1406..2779
                     /locus_tag="VFY96_11595"
     CDS             1406..2779
                     /locus_tag="VFY96_11595"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX5607154.1"
                     /translation="METYSQIIGRFAGGLKLAAVPPAVIEKAKLVFLDTLGIALASST
                     MDFGAMVTNVARKLGGPKASLLIGTPERVAAANAVMANGTLAHGLDYDDTLEEAIVHT
                     GCCAGMTALAVGEEVGASGAAVLEAAIVGTEVLCKVGLVAPGKFHARGFHPTAICSTF
                     GAAAAAGNLYGLTLNQWVDAFGLCGSQSSGIIEYLADGTWTKRIHPGWSAHGGVIATL
                     LAKEGFRGPAKVFEGTHGFFSAFGGTDNYRFEKMSELGKQWEIPRLTFKSYPCGSISH
                     PYMDCALRINQKHSPPPEEIAEVVCRTAEGPVHRLWEPLADKRKPVSSYGAKFSLPFS
                     IAIMLVRGRAGLEEFSDAAINDPKLLDLAAKVRYALDPAIDYPRHFEGHVTVKMNDGR
                     VFDENQLHPRGGFEDPLPPGEIEEKFRANAGLVLSRSKVDEIVAMVSRLEALPAISIL
                     TEKLMPS"
     gene            2818..3840
                     /locus_tag="VFY96_11600"
     CDS             2818..3840
                     /locus_tag="VFY96_11600"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5607155.1"
                     /translation="MKNINVWLIALWGIFVLAAPVWSQDAALVQAARKEAKVVWYTSL
                     AIPSSTAISHYFQNKFKGIEVEVHRTGSQRVLQRAMQEATAGIKNVDIIHTSDAGHFE
                     LLKDKGMLLKFTPQAVSAFPDGFKDKDGFYYGMRATLSVIAFNPKIVAEKDAPKTWKD
                     LLDNKWSGKEVTAHPGYSGIIMTHVLALVNAYGWDYFRDLAKNKLHIVQSANDPAGVV
                     ASGERPVGVNGAEYFYYKTLKQGNPIKIVYPKEGVPLVVSPVAIAKDAPHANAAKLFT
                     EFIFSKESQQLLADKEGLYTGHPEVTYPTDKPKLKDLKLLNADADELEKRNAEIKKRF
                     TEYFGA"
     gene            4147..5874
                     /locus_tag="VFY96_11605"
     CDS             4147..5874
                     /locus_tag="VFY96_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX5607156.1"
                     /translation="MAASETETIERSAHTGWPALRFMDPTLPVWIGAALLLVLLMILP
                     LAAIFRASLWDESGISFGKYLEVFTNGQFLKAIWNTLIISTWVGVISVIVGALLAWLV
                     TRTDLPWKKATRALVMASFVTPPFLGAFAWTLLAGPNAGALNKLYRAVSGSEGSLFNI
                     FTMPGLIFVMTLYSFPYVFSMIANVCELMSSDLEDAAEILGANKWRTAMTVTLPLAMP
                     ALIGGFILAFLQSLSLFGAPAILGLPAGLHTITTQIWALFQYPPRLDMAAAFSVPLLL
                     ATIGLIALQKKILGRKGYSTVGGKGGQKRLIRLGWGRVPVLLFVLGILSLSVFLPYWI
                     LFKAALSKAWAVPLTWDNFTLHNLSFTFFEYGDTQLAIYNTFKLGLLTATVGTLLATL
                     IAYMSNRKLFRGAQYLSFFALAPLVIPGIVLAVGLFIVYTTPPLVLYGTIWILFVAFL
                     TKEMPIGFSQAESTFKSIHPELEDASRIIGAHRLISLKDITAPLARSGLIAAWSFIFI
                     GVIRELSAAILLFAPQSKVVSVVIFDLKEEGQVGVIAVLGILLLVVSFAVILAVQWFA
                     GRDVIATRE"
     gene            5876..6961
                     /locus_tag="VFY96_11610"
     CDS             5876..6961
                     /locus_tag="VFY96_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5607157.1"
                     /translation="MAVVSIRELSKKFAEAAAVDNLDLEIGDGEFVSLLGPSGCGKTT
                     TLRLLAGFLQPDAGEIRVGGEVVSSRSLLVPPERRQMSMIFQSYAIWPHMTVAQNVAY
                     GLKFKKLARPEAEAKVTKLLSVVHLTEFRDRYAAELSGGQQQRVALARALVVEPQILL
                     MDEPLSNLDANLREEMRFEIRRLHEEFKITTVYVTHDQAEAMATSDRIAVLDHGRLIQ
                     VGRPGEIFDRPKTRFVAEFVGKANILTGSYNGDGLLQLNDGTKIRVSGDGPAARSANA
                     AVCLRPHNILLVSNDAAARQLRERGYNVFSGTIGRSIYFGDAVDYTIELPSQRSLRVI
                     APPSQRFDAGQQIHAAAHPEHCVVVRE"
BASE COUNT         1483 a         1948 c         2103 g         1563 t
ORIGIN      
        1 caacggcaga ttcgccagat ggggggcagt gcggaatgct gtgtactcgg cacttcggtt
       61 ttggcgccgc cagcgaaggc cgcattggcc aatggcgtcg ccggtcatgc gatggattac
      121 gatgacacgc aattatccac ttccaaagaa gcagtttatg ggctgttgac ccatccgacc
      181 acgccggtgt tggcggcagt tttagcgctg ggtcaaaaac gcaaaatcac cggcgcggag
      241 tttcttttag catacgtttt gggtgtagaa gtcgaatgcc gcatcgccga tgcgatcgat
      301 ccgcaccatt accaatccgg ttttcattcg acggcgacca tgggcggtct cggcgcggcg
      361 atggcggcgg gtaaaattct tcgtctgaaa gaaacacaac tgcttgcgac attgggtatc
      421 gcagcgagca tggcgtctgg gctccgggaa aattttggca cgatgaccaa gccgctgcat
      481 gcggggcggg ccgctgagaa cggcgtcacc gccgcgctgt tggcgcggga tggctttact
      541 gcggctgcca atatcctcga ggcccgccgc ggctttttta acgcaatggc cggcggttat
      601 gatgatagca agattagcgg ccgtttgggg tctccctatt ttatgaaaga gcctggcatt
      661 tccattaaac cgtacccttc gggctcgctc tctcatccgg cgcaggattt gattttggat
      721 ttggtcaaac atcatgatgt caaagcgaaa gacatcgaac ggatcgatgt cggtaccaac
      781 tcgaatgtgc cgaatgcgct gatctatccg atgccaaaga cggcattgga gggaaaattc
      841 agcatcccgt tctgtatggc catcggcgtc ctcgagcgaa aagcggggat agcgcagttt
      901 caagaccgta aggtacgcga taaaaatgtc gtcgatttaa taaagcgggt aactctctat
      961 gtcgatgaag agctggaacg actcggctat gatcaggtgc gctctcggat tcgtattaag
     1021 cttagagacg gccggattat cgaaggacgt tatgacgtcg cgcgcggcca tcctacaaag
     1081 ccaatgagct ggtcggagct tacggagaaa ttccgcgatt gcgcgggttt ggttttggcc
     1141 aagaagaata tcgaagagac gattcgcctg gttgaaggat ttgaaaactt gaagagcctg
     1201 cggccgctcg tccgcgcgct cacgaatggg caggaaaaaa gaaccatgaa acataaataa
     1261 tgggttgtgt ttgctttgag ccgaatgatg tagagaaaga ttcctcgctc tgctcggaat
     1321 gacaactcaa caggggcttg tcgtcccgaa cagaagtggg agatctttcg ggaacgaagc
     1381 atagaaaaag accggcagat ctcgaatgga gacttactcg caaattatcg ggcgattcgc
     1441 cggcgggctt aaactcgccg ctgtgccgcc agcggtgatc gaaaaagcca aactggtctt
     1501 tctcgatact cttggaatcg cgctggcatc gtcgacgatg gatttcggcg cgatggtaac
     1561 caacgtggcg cgaaaactcg gcggcccaaa ggcaagcctg ttgatcggca cgcctgagcg
     1621 tgtggctgcc gccaacgcgg tgatggccaa cggcacattg gcgcatggcc tcgactacga
     1681 cgataccctt gaagaggcga tcgtgcatac agggtgctgc gcgggtatga ccgcgcttgc
     1741 tgtcggcgaa gaggtcggcg cctccggcgc cgcggtgctc gaagccgcga ttgtcggtac
     1801 ggaagttctt tgcaaagtgg gtctcgtcgc tcccggaaaa tttcatgcgc gcgggtttca
     1861 cccgaccgca atctgctcga ccttcggcgc cgctgccgcc gcgggcaatc tctatgggct
     1921 aactttgaat cagtgggtcg atgccttcgg cctatgcggg agccagagct cgggcattat
     1981 tgaatatctc gcggacggca cctggacgaa acgcatccat cccggatggt cggcccacgg
     2041 cggcgtcatt gcgacgctgc ttgcgaaaga aggtttccgc gggccagcca aagtcttcga
     2101 agggacgcac ggtttcttta gcgcgttcgg cggcacggac aattaccgct tcgaaaaaat
     2161 gtccgagctc ggtaaacagt gggagatacc gcggctgacg ttcaagtcct atccttgcgg
     2221 ctcgatctcg catccttaca tggactgcgc gctcaggatc aatcaaaagc actcgccgcc
     2281 gccggaagag atcgccgaag tcgtttgccg cacagccgag ggtccggtgc accgcctctg
     2341 ggagccgctg gcggataaac gaaagccggt gagctcctat ggcgcgaagt tcagcctgcc
     2401 ttttagcatt gcgatcatgc tggtgcgtgg ccgggcgggt ctggaagaat tttcggatgc
     2461 ggcgataaac gatccgaagc ttctggatct ggcggcgaag gttcgttacg cgcttgaccc
     2521 ggcgatcgat tacccgcgcc atttcgaggg gcatgtgact gtgaaaatga acgacggcag
     2581 agttttcgac gaaaaccagc ttcacccgcg cggcggcttc gaagatccat taccgccggg
     2641 cgagatcgag gagaagtttc gcgccaacgc gggcctggtt ttatcccgca gcaaggtcga
     2701 tgaaatcgtc gcaatggtca gccgcctcga ggcgctgccg gcaatcagca tattgactga
     2761 aaaattaatg ccgtcataaa tggcggctaa cattccatgg gggctcagga gggaactatg
     2821 aaaaacataa atgtttggtt gatcgcgcta tgggggatct ttgtcttggc ggcacccgta
     2881 tggtcgcagg atgccgcgct ggttcaggcg gcgcgcaagg aagccaaggt cgtttggtac
     2941 acttctctgg cgattccttc atccacggcg atctcacatt acttccagaa taaatttaaa
     3001 ggcatcgaag tcgaagtgca ccgcaccggt tcccagcggg ttttgcagcg cgccatgcag
     3061 gaggcaaccg ccggaatcaa aaacgtggac atcatccaca cctcggatgc cgggcatttc
     3121 gagcttctaa aagacaaggg catgctgctg aaattcaccc ctcaggccgt gagcgcgttt
     3181 cccgatggtt tcaaggacaa agacggcttt tactatggga tgcgcgcgac gttatcggtt
     3241 atcgcgttca atccgaagat cgtcgcggag aaagacgcac cgaaaacgtg gaaggatctc
     3301 ttggacaaca agtggagcgg caaagaggtg acggcgcatc ccggttacag cggcattatc
     3361 atgacccacg tgctcgcgct ggtgaatgcc tatggctggg actacttccg cgatctggcg
     3421 aaaaacaagc tgcatatcgt gcaatcggcc aatgatccgg ccggcgtggt tgcgtcgggc
     3481 gagcgcccgg tgggagtgaa cggcgcggag tatttttatt acaagacgct aaaacagggc
     3541 aatcccatca agatcgtcta tcccaaagaa ggcgtgccgc tggtcgtctc gccggtggcg
     3601 atcgccaagg acgcgccgca cgccaacgca gcaaaacttt tcaccgaatt tatcttttcc
     3661 aaggaatcgc agcagttgct ggcggacaag gaaggccttt ataccgggca tccggaggtc
     3721 acgtatccga ctgacaagcc gaagctcaag gatttgaagc ttctaaacgc ggatgccgac
     3781 gagctggaaa agcgcaatgc cgagatcaag aagcgcttta cggaatattt cggcgcatag
     3841 cagcggataa tactgcctcc tatgcaactg catttcgtgg gtcgaactgc gtgtcggccc
     3901 gagggggcgg atacatgggg tccgccccta caaacaaaac atttaggagg ttcggtttaa
     3961 gatatagaaa aacatgacct tcgtatccca aagtgtcacc ctcgaatgtt tttatcgggg
     4021 gtccagttcc gaatttgcct gtggtgaggt tctcggacta ccgctaaatg catgtgggaa
     4081 cagtgggctg ggcatttagc gccacgtcga aagctgcggg atcgaaccca aacggtaaca
     4141 atcttcatgg ccgcttccga aaccgagaca attgagcgct ccgctcacac cggttggccg
     4201 gcgcttcgct tcatggatcc aaccctgccc gtctggatcg gcgccgcgct cctgctcgta
     4261 ttgttaatga ttctcccgct ggcggcgatt ttccgcgcca gtctgtggga cgagagcggc
     4321 atcagcttcg gcaaatacct cgaagttttt accaacggcc agtttctcaa agcgatctgg
     4381 aataccttga ttatttccac ctgggtcgga gtgatctcgg tgattgtcgg cgcgttgctt
     4441 gcgtggctcg tgacgcgcac cgatctgccc tggaaaaaag caacccgcgc gctggtgatg
     4501 gcatctttcg ttacgccgcc gtttctcggc gcatttgcct ggacgctcct ggcggggccg
     4561 aatgccggtg cgctgaacaa gctttaccgt gctgtcagcg gttcggaagg ctcgctcttc
     4621 aacatcttca ccatgccggg gctgatcttc gtgatgacgc tttacagttt tccatacgtt
     4681 ttttcgatga tcgccaatgt ctgcgagctg atgtcgtccg atctcgaaga cgccgcggaa
     4741 attttgggcg cgaataaatg gcggacagcg atgaccgtca cgcttcccct ggcgatgcct
     4801 gcgctgatcg gcggtttcat cctcgccttc ctgcagtcgc tctcgctctt cggcgcgccg
     4861 gcgattctcg gtcttcctgc cggcctgcac acgatcacga cgcagatctg ggcgctgttt
     4921 caatatccgc cgcgcctgga tatggccgcc gcgttctcgg tgcctctgct tttggcaacg
     4981 atcgggctga tcgcgctgca aaagaaaatt ctcgggcgca aaggctatag caccgtcggc
     5041 ggtaaaggcg ggcagaaacg actgatccgg ctcggctggg gtcgggtgcc cgtgctgctg
     5101 tttgtgctcg gcatattgtc gctttcggtg ttcttgccgt actggatcct gtttaaggcg
     5161 gcgctttcca aagcttgggc ggttccattg acctgggata atttcacgct gcacaatctt
     5221 tcgttcacct tttttgaata cggcgatacc caactggcga tttataacac tttcaaattg
     5281 ggtctattga cggctactgt cggcaccttg ctggcgacac tcatcgccta catgagcaat
     5341 cgcaagctgt ttcgtggcgc gcagtacctg agtttcttcg cgctggcgcc gttggtcatt
     5401 cccggcattg ttttggccgt cggcttgttt atcgtctata cgacgccgcc gctggtactc
     5461 tacggcacca tctggatact gttcgtggct tttttaacca aagaaatgcc catcggattt
     5521 tcccaggcgg aatcgacttt caaaagcatc caccccgagc tcgaagacgc cagccggatt
     5581 atcggcgcac accggctcat ctcgctcaag gacatcactg cgccgctggc gcgctcggga
     5641 ctgatcgccg catggtcgtt tatttttatc ggcgtcattc gcgagctcag cgccgcgatt
     5701 ctgttgttcg cgccgcagtc taaggttgtg tccgtggtta ttttcgacct caaagaagaa
     5761 gggcaggtcg gagtgatcgc ggtgctcggg attctcttgc tggtggtttc cttcgcggtg
     5821 atcctcgcgg tccaatggtt tgccgggcgc gacgttatcg cgacacgaga gtaggatggc
     5881 ggtcgtttcg atacgagagc tgagcaagaa atttgccgaa gccgcggcgg tcgacaacct
     5941 cgacctggaa atcggcgacg gcgaatttgt ttcgctgctc ggtccgtcgg gctgcggtaa
     6001 aaccacgacc ttgcgcttgc tcgcgggctt tctgcagccc gacgccggag agattcgcgt
     6061 cggcggcgag gtcgtctcgt cgcgctcact gctggttccg ccggagcggc gccagatgtc
     6121 gatgatcttt caaagctatg cgatctggcc gcacatgacg gtggcgcaga acgtcgcgta
     6181 tggcctcaag ttcaaaaaac tcgcaagacc cgaagccgaa gcgaaagtaa cgaagctgct
     6241 ctccgtcgtg catctgacgg aattcaggga tcgttatgcc gcggagcttt ccggcggcca
     6301 gcagcagcgg gtggcgctgg cgcgcgcctt ggtcgtggaa ccgcagattc ttttgatgga
     6361 cgagccgctc agcaatctgg acgcgaacct gcgcgaagag atgcgcttcg aaatccgccg
     6421 gctgcacgag gaattcaaga tcaccaccgt gtacgttacc cacgatcagg ccgaagcgat
     6481 ggcgacctcg gatcggatcg cggtgctcga tcatggccgt ctcatccagg ttggaaggcc
     6541 tggggaaatc ttcgaccggc caaaaacccg ctttgtcgcc gagttcgtcg gcaaagcgaa
     6601 cattctcacc ggcagttaca acggcgacgg cttgttgcag ctgaacgacg gaaccaagat
     6661 tcgcgtgagc ggcgacggcc cggccgcccg ctcggcgaac gcggccgtgt gcctgcggcc
     6721 gcacaacatt ctgctcgtat cgaacgatgc cgcagcgcgc caactccgcg agcgaggcta
     6781 caatgttttt tcaggcacga tcggccgttc gatttacttt ggcgacgccg tcgactacac
     6841 gatcgagctg ccgtcgcagc gatcgctgcg agtcatcgcg ccgccgtcgc aacgcttcga
     6901 tgccggccaa cagatccacg ccgccgccca tccggagcat tgcgtcgtcg ttcgagagta
     6961 gcgctttaag aaatcgcttc gtagccgcgc gggcgctcac tttgactagc catcgcggtg
     7021 gaaattattc ggcgttgacc gtccgtagag atttggatgc aagactgaat aacggaggtg
     7081 agccatggtc acagcgt
//
LOCUS       DASULZ010000566         2990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_369375, whole genome shotgun sequence.
ACCESSION   DASULZ010000566 DASULZ010000000
VERSION     DASULZ010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369375"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VFY96_11615"
     CDS             complement(<1..484)
                     /locus_tag="VFY96_11615"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607158.1"
                     /translation="MAYRTVGKALPRIEGYGKVTGQTKYAADLEFPELLWAKVLRSPV
                     PHARIANIDTSKAKALKGVHAVLTGDDVKDIYVGTRVKDQPVLVHDKVRMCGDAVAAV
                     AALSAAVAAEAIGLIDVEYEELPHVDDPLEALKPSAPLIHEDRSKYKNAPKLPEGVSP
                     H"
     gene            complement(610..1722)
                     /locus_tag="VFY96_11620"
     CDS             complement(610..1722)
                     /locus_tag="VFY96_11620"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5607159.1"
                     /translation="MKNGFKIVDSDMHLREPADLWEKYMEPEWRERAPKILSSTARSS
                     AMVMLDGKILQGYKPTYRGGIYDATRIDKEIGDFRARGFDARTQLDAMDREGLDVAAL
                     YPSIGLGIMMRENIDPKLAAAIARAYNNWLHDFCQADPKRLKGVAMIPLHDPAEAVKE
                     AHRAVDKLGFVGVFSRPEPFNNLPWHSRYFDTLWSALEELAVPIGFHSATAAGEVPQI
                     GDRFGENLLLRHVVSHPMENMMALVDIIGGGVCERHPKLKVAFLECYCGWVSFLLHRL
                     DNAMAKGRFPTAGKLKPTEYFARQCWISTEHEKELPMIIDLIGDDNIVFSTDYPHGDS
                     DFPEAVEEFLEMSGVSDQSRKKILWDNCARLYGLGD"
     gene            2083..>2990
                     /locus_tag="VFY96_11625"
     CDS             2083..>2990
                     /locus_tag="VFY96_11625"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5607160.1"
                     /translation="MFDFLFPNHPFGGHTLRLVAQAQQGGGDIFDIARAMKTVEAGDR
                     AGWESAWLGLAEKTEARAHQALAANRLRTARQYFFHASNYYRMSDVLLTIAEEPKRRE
                     RFRKSQELFREAAKLNDTKIEVITVRSENEEYDGYFCHPTGSQPGKWPAVLFLGGADA
                     YAEEIYFGGKQMLDRGWAMLLVDTPGRGSSIYLKGIKTRPDYEVPGKACIDYLLSRPE
                     VDPNRVALMGISMAGYYAPRVAAFEKRIKALIAWSGCYSVLNDLYDFCAHLQPVCQRL
                     LGGVSHEEARRQLKDYTMQGIVENIT"
BASE COUNT          660 a          850 c          801 g          679 t
ORIGIN      
        1 tgtgaggcga cacaccttcg ggcagtttgg gtgcattttt atatttgctt cgatcttcgt
       61 ggatgagcgg cgccgagggc ttgagcgctt cgagcggatc atccacgtgg ggcaactcct
      121 catattcgac atcgatcaat ccgatcgctt ccgctgcaac cgctgcgctg agagcagcga
      181 ccgccgcgac agcatcacca cacatccgta ccttgtcatg gacgaggacc ggctgatctt
      241 tgacgcgcgt gccgacataa atatctttca cgtcgtcgcc ggtgagcacc gcgtgcacgc
      301 cctttaatgc tttggctttg gaagtatcga tattggcaat gcgcgcgtgc gggactgggc
      361 tgcgcaacac ttttgcccac aaaagctcag ggaactccag atcggcagca tatttggttt
      421 gccccgtgac tttgccgtaa ccctcgatcc gaggcaacgc ttttcctacg gtacgataag
      481 ccaaggttta cctccaacat tttggatttt agattttgga tttgagactg ccgggaaaag
      541 aaatcagaga ttcgctactg ggatgtcttc cctaatccaa atccaaaatc gaagtgtaaa
      601 attacgtcgc tagtcgccca gtccgtacag tcgagcgcag ttatcccaga gaattttctt
      661 ccggctctgg tccgacactc cgctcatttc gagaaactct tctaccgctt ccggaaagtc
      721 cgaatcgccg tgcggataat cggtcgagaa cacgatattg tcgtcgccaa tcagatcgat
      781 gatcatcggc aattcctttt cgtgttcggt cgagatccaa cactggcgcg caaaatactc
      841 ggtcggtttg agttttccgg cggtcggaaa tcgccccttg gccatcgcat tatccaagcg
      901 gtgcagcaga aacgagaccc aaccgcaata gcactccagg aacgccacct tgagtttggg
      961 atgacgctcg cacacgccgc cgccgatgat atccaccagc gccatcatat tctccatggg
     1021 atgagagacg acatggcgca agagtagatt ttcgccaaaa cgatcgccaa tctgcggtac
     1081 ttcgcccgct gccgtggccg agtgaaaccc gatcggcaca gccagctctt cgagcgccga
     1141 ccaaagcgta tcgaaatacc gcgaatgcca cggcaagtta ttgaacggct cgggccgaga
     1201 aaaaacgccg acaaagccca gcttatcgac cgcgcgatga gcttccttga ccgcctccgc
     1261 cgggtcgtga agcggaatca tcgccacgcc tttcaggcgt ttcggatcag cctggcaaaa
     1321 atcgtgcagc cagttgttat aggcgcgcgc gatcgccgcg gcgagcttcg gatcgatgtt
     1381 ttcacgcatc atgattccca ggccaatgga cggatagagt gccgcgacgt cgagtccctc
     1441 tcggtccatc gcatcgagct gcgtcctggc gtcgaaccca cgagcgcgaa aatccccaat
     1501 ttctttgtcg atgcgagtgg catcgtaaat cccgccgcga taagtcggtt tgtagccttg
     1561 aagaatttta ccgtcgagca ttaccatcgc cgaactgcgc gccgtcgagc taaggatttt
     1621 cggtgcgcgc tctcgccact cgggctccat gtatttctcc cagagatccg ccggctcgcg
     1681 cagatgcatg tccgagtcga caatcttgaa accatttttc atcaaataac ctccgatgat
     1741 cttgtgagta tcatgatcgc gtgcctcgct gtcaatacga cattcccgtc ctctggctct
     1801 tgattgtcat gccgagctaa cagagcgagt gaagactccg cccagagtcg ctcaggatcc
     1861 ctcgatccgc gcagcgcgct actcgggaaa tcggttcatc cgattatcct gagtaggcag
     1921 cgagcagcgc tgcgtatcga aggacgaacg gcggtgtttt cacagcctgt caaggcatgg
     1981 aatcttgccc ttgatcggca accatagatt gcatacgcgc aggattcatg gactttgcca
     2041 ccctttatgc ataagatcag cttcgttctt tcggggtcaa cgatgtttga ttttctgttt
     2101 cccaaccatc cattcggcgg gcacacactg cggctggttg cgcaggcgca acagggcggc
     2161 ggtgatattt ttgatatcgc ccgcgccatg aaaactgtcg aagcaggcga tagagcgggt
     2221 tgggagagcg cttggctggg tctggccgag aagaccgaag cacgcgctca ccaggcgctt
     2281 gccgccaacc gattgcgcac cgcgcggcag tatttctttc atgcaagcaa ctactaccgt
     2341 atgtccgacg tgctgctcac gattgcggaa gagcccaagc gcagggaacg gttccgcaaa
     2401 tcacaggagc tcttccgcga agccgccaag ctcaacgaca ccaagatcga agtgataacc
     2461 gtgcgatcgg aaaacgaaga gtacgacggc tacttctgtc atccgacagg gtctcaacca
     2521 gggaaatggc cggcggtgct ctttctcggc ggtgccgatg cctacgctga agaaatctat
     2581 tttggcggta aacagatgct cgaccgcggc tgggcgatgt tgctcgtcga tacgccggga
     2641 cgtggttcgt ccatttatct aaaaggcatc aagacgcggc ctgattacga agttcccggc
     2701 aaggcttgta tcgattatct cctttcacgt cctgaggtcg atccaaaccg cgtcgcgctg
     2761 atgggtatca gcatggcggg ttattacgct ccgcgtgtgg cggcatttga aaaacgcatc
     2821 aaggctttga tcgcctggag cggctgctac agcgtgctca atgatcttta tgatttttgc
     2881 gctcacctgc agcctgtttg ccagcgcttg ttgggcggcg tgagccatga agaagcgcga
     2941 cgacaattga aagattacac gatgcagggc atcgtcgaaa atatcacctg
//
LOCUS       DASULZ010000567         3133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_573829, whole genome shotgun sequence.
ACCESSION   DASULZ010000567 DASULZ010000000
VERSION     DASULZ010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573829"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VFY96_11630"
     CDS             <1..465
                     /locus_tag="VFY96_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5607161.1"
                     /translation="ILASTGAAFPGATGLPLTMVKEMVEWGYPKYVREHTIQVGFTDE
                     FITTHTDRVEEFFRTRLANLCPVEFYLRHVFVRQGHDTRDRLKNIRVPTLVMVGEHEG
                     DPNLAMTHRASSEMLAKAIPGAQFVILPGQKHDYLASAPETAHRIIRDFLRQ"
     gene            complement(514..843)
                     /locus_tag="VFY96_11635"
     CDS             complement(514..843)
                     /locus_tag="VFY96_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607162.1"
                     /translation="MQFCRLYTGDDGRSHFEDLDQTEGSKYFLAALPAKALVFKNDKN
                     RDDLHGWHTAPRRQWCITLSGTVEIGIGDGAKRVFGPGDVFLAEDVTGEGHTAVPVNW
                     VRAFVHL"
     gene            885..1103
                     /locus_tag="VFY96_11640"
     CDS             885..1103
                     /locus_tag="VFY96_11640"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5607163.1"
                     /translation="MAAKFQAIMLIRSFRHLRMIPHPIDAAIRLLDIGAGYGALATAV
                     LLERPNSVVICLDASEAMLKLGRERNTS"
     gene            1134..1523
                     /locus_tag="VFY96_11645"
     CDS             1134..1523
                     /locus_tag="VFY96_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607164.1"
                     /translation="MNQPSGVIRRWPFRCRRLFARVASLYRTRTPRDIFKEVFSLLRR
                     DGCFINADNIRPPPESLKERYRRARDEYLDEFVPRSSGGKTNLAEVRAATPSSYRPHN
                     NGYLEQELAWFKEAAFVDVDCFGSLPP"
     gene            complement(1609..2367)
                     /locus_tag="VFY96_11650"
     CDS             complement(1609..2367)
                     /locus_tag="VFY96_11650"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017305739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5607165.1"
                     /translation="MSQSTTWNPESYARNARFVSELGEPLLQILDPKPGELILDLGCG
                     DGTLGEKIAGRSKLVGVDSSMPFLKSARGRDLTVVQMDGQQLAFKRCFDAVFSNAALH
                     WMNRPEKVLEGIANCLKPCGRFVGEFGGQGNVETIRAALHAGLQKRSIDPWQVDPWYY
                     PSPETFTALLERFGFSVGYIELIPRPTKLPGDIIQWLALFAQPFTKSLADGDKRRFVD
                     EVRDALEPELRRSDGSWYADYVRLRFKAELRLAW"
     gene            2548..>3133
                     /locus_tag="VFY96_11655"
     CDS             2548..>3133
                     /locus_tag="VFY96_11655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5607166.1"
                     /translation="MLVAVGLALKHWLDPGLEVQLATATLTSPSQANAVLTASGYVVA
                     RRKAAVASKGTGTLVYLAVEEGDRVKKGQVIARLDDSDVMATLRRAQENLRVAEADLD
                     DARKHYERMRAVIDKRAVSQADYDAAEARYKRVVSTIGAAKYAVREAEVAVANTRIVA
                     PFDGTVLKKNADVGEIVAPLAGAASSKAAVVTIAD"
BASE COUNT          696 a          862 c          872 g          703 t
ORIGIN      
        1 attcttgcct ccaccggcgc ggcgtttccg ggcgccacgg gtctacccct cacaatggtg
       61 aaggagatgg tcgaatgggg ctatccgaag tatgtgagag agcatacgat tcaagtgggg
      121 tttacggacg aattcatcac aactcacacg gatcgagttg aagaattctt tcgaacccgg
      181 cttgccaacc tttgtccggt agaattttac ctgcgccacg ttttcgtccg gcaaggccat
      241 gacacgcgtg atcgactgaa gaatattcgg gtgccgactt tggtcatggt gggagagcac
      301 gaaggcgatc cgaatctggc gatgacgcat cgtgcctcat cggagatgtt ggcgaaagcg
      361 attcccggtg ctcagttcgt catattgccg ggccagaaac acgattactt ggccagcgct
      421 ccggagacag cgcatcgaat cattcgcgat tttttacgcc aatagaaatt cttctgaaaa
      481 ataggttcgt aattttcgcc attatctaga tgcttataag tgaacgaacg cccgcaccca
      541 attcacgggc acggctgtgt gaccctcgcc ggtgacgtct tcggcaagaa aaacatcacc
      601 ggggccgaac actcgcttgg cgccgtcgcc aatgccgatc tcgacagttc ccgacagcgt
      661 aatacaccac tgccggcgcg gcgcagtgtg ccagccgtgc agatcgtcgc gatttttatc
      721 gttcttgaac accagcgcct tggccggaag cgccgccaaa aaatattttg atccctcggt
      781 ttgatccaaa tcttcgaagt gagatcggcc atcatcacct gtgtagagtc tgcagaattg
      841 catggagatc ctcccgcggc gcagttttca atcgcttaac agcaatggca gcgaagtttc
      901 aagcgatcat gttgatacgg tcatttcgtc acttgcgcat gattccgcat ccgatcgacg
      961 cggcgattcg cctgctcgac atcggcgccg gttacggagc gttggcaacg gcggtcctgc
     1021 tggagcgtcc caatagcgtg gtgatttgtc tcgatgcttc cgaagcgatg ctgaagcttg
     1081 gaagagagcg aaatacaagt tgaagcagcg catcagcttt attcaaggat cgcttgaatc
     1141 agccgagtgg cgtgattcgt cgatggccat ttcgatgccg tcgtctcttc gcgcgcgttg
     1201 catcacttta ccgaacacga acgccgcgcg acatcttcaa agaagttttc tccctgctgc
     1261 gtcgcgatgg gtgttttatc aacgccgata acatccggcc gccgcccgag agcttaaaag
     1321 agcgctatcg ccgcgctcgc gacgaatatc tcgacgaatt tgtaccccgt tcaagcggcg
     1381 gcaaaaccaa tctagcggaa gtccgcgccg cgactccgag cagttatcga cctcataata
     1441 acggctatct ggagcaagag ctcgcctggt tcaaggaggc agcgttcgtc gatgtcgatt
     1501 gctttggaag tttaccaccc tgatagtcta cggtgggttt cggctttagt tcgagttcaa
     1561 gctgttcaca tcattcaaac cgttcaagcc gttaactagt cattgagttc accaggccag
     1621 gcgcagctcg gctttgaaac ggagccgtac gtagtcggcg taccaactgc cgtccgatcg
     1681 gcgaagctcg ggttcaaggg catcgcggac ttcatctaca aagcgccgtt tgtctccatc
     1741 ggccaatgat ttggtaaaag gttgggcgaa caatgccaac cattggataa tgtcgcccgg
     1801 aagcttcgtg ggccgcggaa tcagctcaat ataaccgacg gagaagccaa accgttccag
     1861 caaagcggta aatgtttcgg gcgacggata ataccaagga tcgacctgcc atggatcgat
     1921 agatctcttt tgcaatccag cgtgaagcgc cgctcgtatg gtttcgacat tgccttggcc
     1981 gccgaattcg ccgacgaatc gtccgcacgg tttgaggcaa ttggcgatac cttcgagcac
     2041 cttttctggc cggttcatcc agtgcagcgc cgcatttgaa aacactgcat cgaagcaacg
     2101 cttgaacgcg agttgctgac catccatttg tacaacggtc agatcgcgac cgcgcgctga
     2161 tttcagaaac ggcatactgg aatccacgcc cacgagcttg ctccggccgg cgattttttc
     2221 gccgagcgtg ccgtcgccgc atccgagatc cagaatcagc tcaccgggtt taggatcaag
     2281 aatttgcaat agcggctcgc cgagctcgga aacgaaacgc gcgtttcgcg cgtagctctc
     2341 cggattccac gtcgtcgatt ggctcatagg agcaaagtag catccggcga ttgtcttgca
     2401 aaaccaggaa ttccccgtag tctgaaatat tttatggcta ctcggggctc ggacttgtcc
     2461 tcgttgagga tcgatcgctc agcgagtcag ttgccacaca aacggcggtc atatagtcga
     2521 atcgttatct ggacgacagc cgttcttttg ctggttgcgg ttggtctggc gcttaagcac
     2581 tggctcgatc ccggccttga agtccaactc gccacggcca cacttacatc tccgtctcaa
     2641 gccaacgcag ttttgacagc cagcggctat gtggtcgcgc ggcgcaaagc cgcagtcgcc
     2701 tccaaaggta cgggcacgct ggtttatctc gccgtggagg aaggggaccg cgtcaagaag
     2761 gggcaagtga ttgctcggct ggatgattcg gacgtcatgg ccaccttgag gcgcgcgcaa
     2821 gaaaatctgc gggtcgccga agcggatctc gacgatgcta ggaagcacta cgagcgtatg
     2881 cgcgccgtga tcgataaaag ggcagtttcg caggcagatt acgatgcggc ggaggcgcgt
     2941 tataagcgcg tggtttccac cattggggcg gccaaatacg cggtccgaga agccgaagtc
     3001 gctgtcgcga atacacgcat tgtagcgcct ttcgacggca ccgtattgaa gaagaatgct
     3061 gatgtcggtg agatcgtcgc gccgttggcc ggagctgcga gctccaaagc cgccgtcgtg
     3121 accattgccg aca
//
LOCUS       DASULZ010000568         2863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_167416, whole genome shotgun sequence.
ACCESSION   DASULZ010000568 DASULZ010000000
VERSION     DASULZ010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167416"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VFY96_11660"
     CDS             <1..448
                     /locus_tag="VFY96_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607167.1"
                     /translation="GAELRQMNEDATSDDFVPRDVPKRPSQPPIRTIQAESGRVVESA
                     LVNQNHYRSRVEMSELGKVDLQQFWFPGWQATVDRLPVKTGPSGPQTVVSCDVPAGEH
                     LVEFSYSSLPQRRTGVTISIVSAAIGACVLGFLRRHRYKGLPVRNR"
     gene            complement(465..848)
                     /locus_tag="VFY96_11665"
     CDS             complement(465..848)
                     /locus_tag="VFY96_11665"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX5607168.1"
                     /translation="MRFRGLLIGFGFILMGMCAAAGQTPSGVESGIEGSITISPAKPG
                     PTRIDAGSVPLANATFVVENNNAEVASFTTDDKGHFRVSLPPGHYKVSLKRRKSSIGR
                     FGPFDVEVRPGKTAIVQWECDSGIR"
     gene            968..1618
                     /locus_tag="VFY96_11670"
     CDS             968..1618
                     /locus_tag="VFY96_11670"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX5607169.1"
                     /translation="MKRYLPFIIVIGVALITFGSGALLYRAKLPQVKNIPDSQGVSEK
                     GDASAHIRGNPAAPVTLEEYGDFQCPPCGQFAAFAEELLREYDSRLRVVFRNFPLPGH
                     EHAREAALAAEAAGFQGKFWEMHDTLYKEQSNWSKAPNTRELFESYAGTLGLNMEEFK
                     KDMDSDKARERIDSDHALGDSLGIKVTPTMFINKRALEPQEKNPEGVRAAINAALN"
     gene            1724..2164
                     /locus_tag="VFY96_11675"
     CDS             1724..2164
                     /locus_tag="VFY96_11675"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEX5607170.1"
                     /translation="MTVSRFRTILYTAVAIVAVVGLADATYLAVQALTGETLTCGGSP
                     DCFRVLGSSYAKLGGIPVAMLGALAYFTAFTFATFAAFGYARAPKFLVLTAGAMFLTT
                     LWLLYVQAFLLHAYCRYCLFSAALTFLIAGLLIAVPPSQRRSGG"
     gene            2185..>2863
                     /locus_tag="VFY96_11680"
     CDS             2185..>2863
                     /locus_tag="VFY96_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal response regulator transcription
                     factor"
                     /protein_id="HEX5607171.1"
                     /translation="MSPTRILVIEDEKKTAAFLTKGLREAGFAVDVAIDGESGLKQAR
                     ATKFDLLIVDIMLPHKDGWDVVAELRRDEVRTPILFLTARDSVRDRVKGLELGADDYL
                     VKPFAFTELLARIRSVLRRAPQQQPELLRIEDLELDTERHKAMRSGVQLNLTPKEFLL
                     LAHLVRSTGAAVSRAEIAEHVWDVGFTTDTNVVDVVVRRLRAKVDDPFKTKLIHTIRG
                     VGYVLKGR"
BASE COUNT          612 a          790 c          808 g          653 t
ORIGIN      
        1 gggcgccgaa cttcggcaaa tgaatgagga cgcgacgagt gacgatttcg tccctcgcga
       61 cgtacccaaa cgaccaagtc agccgccgat ccggacgata caggccgaga gtgggcgcgt
      121 tgtcgagtcc gcgctagtaa atcaaaacca ctatcgtagc cgagtcgaaa tgtccgagct
      181 cgggaaagtc gacctgcagc aattttggtt ccccgggtgg caagccacgg tcgatcggct
      241 tccggtcaaa accggtccct ccggccctca aaccgtggtc tcctgcgacg tacccgcggg
      301 ggagcacctt gtcgaattca gttatagcag tcttccgcaa cgcagaactg gcgtcacgat
      361 ctcaattgtc tctgccgcaa ttggcgcctg cgttcttggg tttctccggc ggcaccgata
      421 caagggccta ccggttagga atcgctagac gagcgtgatc taccttaccg aatacccgag
      481 tcgcattccc attgaacgat tgcggtcttg cccgggcgaa cttcaacgtc gaaagggccg
      541 aagcggccga tgctcgattt ccttctcttg agcgagacct tgtaatggcc aggcggtaac
      601 gacactcgaa aatgcccctt gtcgtcagtg gtaaacgacg ccacctcggc attgttgttt
      661 tccacgacaa acgttgcgtt cgccagcggc accgaaccag cgtcgatccg ggttggtccc
      721 ggtttcgccg ggctaatggt gatgctccct tcgattccgg attccactcc ggaaggagtc
      781 tgcccggcgg cagcgcacat gcccatgaga ataaacccga acccgatgag taaaccgcgg
      841 aatctcactg tgcactatat tcacgcagtc ccgcgatggc caatgattcg aaagagaaag
      901 cgaatctcaa ttgggctcgt acttgacgaa ggtatcgtgg cgtcgaaatt ctccgcttct
      961 ctcgcgcatg aaacgatacc tgccgtttat aattgtgatt ggtgtcgcat tgatcacgtt
     1021 tgggagcggc gcgctgttgt atcgagcaaa gcttccgcag gttaagaaca ttcccgatag
     1081 ccagggtgta tcggaaaaag gcgatgcttc ggcgcacatt cgaggcaacc ctgcggctcc
     1141 ggttacgctg gaagagtacg gcgattttca gtgcccaccg tgcggacaat tcgccgcttt
     1201 tgctgaggaa ttattgaggg aatatgattc gcggctgcgc gtcgttttcc gaaactttcc
     1261 gctgcccgga cacgaacacg ctcgcgaggc tgcgttggct gcggaggctg ctggtttcca
     1321 agggaaattt tgggagatgc acgacacgct ttacaaggag caatcaaact ggagcaaggc
     1381 tccgaacacg cgggaattgt tcgaatcgta cgcagggacg ctgggtctca acatggaaga
     1441 gtttaagaaa gatatggata gcgacaaagc gagagaacgg atcgattccg atcatgcgct
     1501 cggcgattct ctgggaataa aggtcacgcc aacgatgttc atcaacaaac gggccctgga
     1561 accacaagaa aaaaatcccg agggtgtccg cgccgccatc aatgcggcgt tgaattgaaa
     1621 gtcgcaatcg tagtcatatg gttaaaccgt tatatcgagt ttccgatttg atctctgaac
     1681 gatttgacgg tccaacgagc cgaaaccagt cgtcattccg gttatgacgg tatcgcgatt
     1741 tcgcaccatt ctttacacgg cagtagccat tgttgcggtg gtcggtctgg ccgacgcaac
     1801 ttatttggcc gtgcaggccc tcactggcga gacccttacc tgcggaggtt cgccggattg
     1861 ttttcgggta ttgggcagtt cgtacgctaa gctgggcgga attccggtgg ccatgctcgg
     1921 cgcgctagct tatttcactg cgttcacttt cgccacattc gcagcgttcg gttacgcacg
     1981 tgcgccgaaa ttcctcgttt tgaccgctgg tgccatgttt ctgacgacac tttggctgct
     2041 gtacgtgcag gcatttttgc tgcatgcgta ctgccgatat tgtttgttct cagcggcact
     2101 tacttttctt attgccgggc tgctcatcgc ggtgccgccc tcacaacgcc ggtctggcgg
     2161 ataagccgcg tgctcgtata ggcaatgagc cctacgcgca ttttggtaat cgaggacgaa
     2221 aagaaaaccg ccgcttttct taccaaaggc ttgcgcgaag ccggctttgc tgtcgatgtc
     2281 gcgattgatg gcgaaagcgg attgaaacaa gcgcgtgcga ccaaatttga tcttttgatc
     2341 gtcgacatca tgctgcctca caaggacggc tgggatgtgg tggctgaact gcggcgtgat
     2401 gaagtccgca cccccatttt gtttcttacc gcccgcgaca gcgtccggga tcgcgtgaaa
     2461 ggactcgagc tcggtgcaga cgattatctg gtcaagccgt tcgcgtttac ggaattactc
     2521 gcccgcatcc gctccgtgtt gcggcgcgct ccccagcagc agccggaact cttgcgcatc
     2581 gaggatttgg aactcgacac cgagcgtcac aaggcaatgc gctcgggcgt tcagctgaac
     2641 ctgacgccaa aagaattcct tctgcttgcg catctggttc gctcgaccgg tgcagccgtg
     2701 tcgcgcgctg aaatcgcaga gcacgtttgg gacgtcggat tcacgaccga cacgaacgtt
     2761 gtcgatgtag tcgtgcgccg gttgcgggcc aaagtagacg acccgttcaa gacgaaactc
     2821 atccacacga ttcgtggcgt cggatatgtt ctcaaaggac gct
//
LOCUS       DASULZ010000569         8018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_472167, whole genome shotgun sequence.
ACCESSION   DASULZ010000569 DASULZ010000000
VERSION     DASULZ010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8018
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472167"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VFY96_11685"
     CDS             <1..315
                     /locus_tag="VFY96_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEX5607172.1"
                     /translation="LLRIFIGESDRYAGMLLYEWIVRTARERGLAGATVLRGVEGFGA
                     RSRVRTAKILDLSTDLPIVVEIVDTAEKIEAFLPVIDVAIRDGLATMERVQIRFYRSR
                     SN"
     gene            392..679
                     /locus_tag="VFY96_11690"
     CDS             392..679
                     /locus_tag="VFY96_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607173.1"
                     /translation="MEEGTKRLFVGLKVTKALQDDLANPAPGTKQYFEGTSDEYLQII
                     SLGEDKFIGRYLKDGFPAANIGDVSRNVCSLVKLITRGRRIEENEVHIYAC"
     gene            complement(878..1399)
                     /locus_tag="VFY96_11695"
     CDS             complement(878..1399)
                     /locus_tag="VFY96_11695"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607174.1"
                     /translation="MFGETGTPIYSLITRLEIKDFADLKGKTVGLSLAVDTISISTRK
                     VMALHAIEEGDFKVKELVGTPARSACLKKNECDAVPLGQPEDFLLMQDGYRRLGVSTE
                     AMSNFQFIVSAVRRSWGNQNKDALIRYARALASSYRYMRAVQNREEVVRIIMDTTGSS
                     ETIARDTLRPQGG"
     gene            complement(1344..1700)
                     /locus_tag="VFY96_11700"
     CDS             complement(1344..1700)
                     /locus_tag="VFY96_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607175.1"
                     /translation="MQPFTFLLAGSIFFAVPHHVHGITLRYGQIPSTLKTVSALQFHL
                     AERKGFFAREGIALEGLPIDGGAANMVLALSKRVVDITRTATPYLIQDVLNGSECRGV
                     RRNRNSDLQPNHQVRN"
     gene            1833..2558
                     /locus_tag="VFY96_11705"
     CDS             1833..2558
                     /locus_tag="VFY96_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607176.1"
                     /translation="MQILQVTLWIYGMALKRAWECVRKNLVVSFAPLVYSLLLSAAAI
                     IALPLGILGGLVVGLVAQACLSSGLFLVKNMIDSGKTDLNDFARGFRVYIWELITISF
                     MLWIPLKLAGMALATVPNGGLIYFCIQIALFVLLNPVPEFIYRSRTSGPELLSASYNF
                     IVENWVEWLTPNIVLAVIGYVLLHQIELALFSLPAFVQLFVHSFALGLFLTYVMTFRG
                     FLFIELYGSSRRSRIYRYKARSD"
     gene            2754..4037
                     /gene="hisS"
                     /locus_tag="VFY96_11710"
     CDS             2754..4037
                     /gene="hisS"
                     /locus_tag="VFY96_11710"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX5607177.1"
                     /translation="MKITSIRGFSDILPEEVGAWQRVETVARRVFEIYNFTEIRIPIV
                     EKTELFSRSIGETTDIVEKEMYTFEDRDGRSAENSEAMKLTLRPEGTAGVVRAYVEAE
                     MYKSEPARKLYYMGPMFRRERPQKGRMRQFHQIGAEALGRADPFIDAEILLLLHDLFA
                     ALKLTDLSLELNSLGDTECRPRYRDELLAFLKAREDSLCGNCRRRIDRNPLRALDCKE
                     PGCIVATKDAPSILEALCVLCRTHFDTVQKLLDETGVRYSLNPRMVRGLDYYCRTTFE
                     WTTTLLGSQSAVAAGGRYDGLVEQLGGPPIPGVGFAMGVERLTMLLKMQEEGIQPGPD
                     LYLVWVGEKARNWAFPVAHTLRQRGLVVEMEGESRSLKSQMRRADKFRAASVLIVGDD
                     ELAKGEGVLRDMASKRQENIKLANIEAELSARKSG"
     gene            4037..5839
                     /gene="aspS"
                     /locus_tag="VFY96_11715"
     CDS             4037..5839
                     /gene="aspS"
                     /locus_tag="VFY96_11715"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX5607178.1"
                     /translation="MAERVFNDPLGDWKRSCYCGEPRAQAVGEEKILVGWVHSRRDHG
                     GVIFVDLRDRTGICQVVFRPEVAASSHEKAKQIRSEDVIAVRGVLAKRSPESINPELA
                     TGEVELVCRELRLLNASAVPPFPIEDESDAAENTRLKYRYLDLRRPQSLSPLLLRYRM
                     TKLIRDYLDGLGFIDVETPVLTKSTPEGARDYLVPSRIYHGKFYALPQSPQLFKQILM
                     VGGLDRYFQIVKCFRDEDLRADRQPEFTQLDMEMSFVQPEDVIEVVEGMMVLLFKELK
                     GVELKRPFKKLTYAEAMNRFGSDKPDMRFGLEIKDFTEALRNSQARVFASAIKAGGVV
                     KGMRIPKGAELSRKDLDDMTPYVATFGAKGIAWTKLTEEGWQSPIAKFLSDAEQKDIE
                     RSAGAESGDVVLFSADQAKVVHDALGNLRLYLGEKLDLIPQNEYGLVWVVDFPLMEFD
                     AEQKRYVALHHPFTAPLDEDLPLLESEPAKSRSKAYDLALNGIEIGGGSIRIHQVELQ
                     KKILALMGISAEEAQAKFGFLLEALSYGAPPHGGIAFGIDRLAMLLSGAKSLRDVIAF
                     PKSQRAVCMLTDAPSTVEPRQLRELGIRLSGGEK"
     gene            5939..6595
                     /locus_tag="VFY96_11720"
     CDS             5939..6595
                     /locus_tag="VFY96_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607179.1"
                     /translation="MLKFTCMALLLLASCSFGPGNLQSRRATDLESRRIRRIAVLPPN
                     EETAPRPRAIPGTTEPRNPERDAPETLTRIIYSVMAAMPNWQIVSDNEVREISQGVAA
                     TGDTARLRRIGEMVYADAVLVSHVRRYRERVGDEWGAKSPASVAFVLDLIDVRRGDIV
                     WSANFDETQKSLSENIFALGDITRRGVRWLSADQLAQEGVKKAVAQLHDMIARAPASS
                     "
     gene            complement(6598..7191)
                     /locus_tag="VFY96_11725"
     CDS             complement(6598..7191)
                     /locus_tag="VFY96_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607180.1"
                     /translation="MIGDPAQFETIGNRLGFEIVENTREKLRLVWHGARFPAFLCLGI
                     AVLLLFVSLPIVEALRLRGFTGPAGALWYFPLMNLILFAIALFLVTQRRVIEVDSRAR
                     RVILRRYSLYRSIVLSASYDEIDKFKLGFDQVYSGFALGGSTAAQKFSVPALRMILRD
                     GAAVLLDRGSVRKLKELGRLASELLSRPLEIEVPGPS"
     gene            complement(7236..>8018)
                     /locus_tag="VFY96_11730"
     CDS             complement(7236..>8018)
                     /locus_tag="VFY96_11730"
                     /inference="COORDINATES: protein motif:HMM:NF039584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase subunit CofH"
                     /protein_id="HEX5607181.1"
                     /translation="VSVRDYLIALKEAGVGSLPGTSAEILVDAIRQRVSPGRIAPAQW
                     IELIQTAHEVGIPTTSTIMYGFVESSRDKSVHLDILRQIQRRTGGITEFVPLSFVYEE
                     APMFHRKPPDGLRRGASGAEVMKMYAVSRLMLNNYIPNLQVSWVKEGPKLSQIALLAG
                     ANDFGGTLINESISTAAGASYGQLMKPSQFRGMIREMSRIPAERSTTYQKIRVSESEG
                     QHRDLLDEVNDPKERFGSYQNLIQLKQYRFRDFYREQKTAKS"
BASE COUNT         1751 a         2104 c         2382 g         1781 t
ORIGIN      
        1 ttgctgagga tctttatcgg cgagagtgat cggtacgcgg gcatgctgct ttacgaatgg
       61 atcgtccgga cggcgcgcga gcgcggcctc gctggggcga cggtgctccg cggagtggaa
      121 ggttttggcg cgcggagccg agtccgcacg gcaaagatcc tggatctctc gacggatcta
      181 ccgatcgtcg tcgaaatcgt cgataccgca gaaaagatcg aggcgtttct gccggtgatt
      241 gacgtcgcga ttcgggatgg tcttgcgacc atggagcggg tgcagatccg attttaccgg
      301 agtcgatcga actagacctt ggttacgctt acgcgggggt gtggttgttt cgcacgtcgt
      361 atgatattga gctagcgaag gagaagtcag gatggaggaa gggactaaaa gactatttgt
      421 cgggctgaag gttaccaagg cgctgcaaga cgaccttgcg aatccggcgc cgggcacgaa
      481 gcaatatttt gaaggcacca gtgacgaata cctgcaaatc atcagcttgg gagaagataa
      541 atttatcggc cgttatctca aggacgggtt tcctgccgcc aatattggcg acgtgagccg
      601 aaacgtctgc agtctcgtca agctcatcac ccgcggccgg cgcatcgagg aaaacgaagt
      661 ccacatctac gcctgctgat ctggcctact gcgcgtgcga agtcccgagc agggcaaatt
      721 ctattcactc ctaatttgcg attcgaccct ttactgcaat cccgcggctt ttaaatattg
      781 taggtcgatg aattgttccg ccggcgggat cggcgattta agctcgccgg cctcggccat
      841 tacgcgtaac acttcattaa atcctttgac gtcgatttca cccgccttgg ggccgcaagg
      901 tgtcccgcgc aatagtttcg gaactgcccg tcgtatccat gatgatccgg accacctcct
      961 cacgattctg cacggcgcgc atgtaacgat aggaagaggc aagggcgcgt gcatagcgta
     1021 ttaaggcatc cttgttctga ttgccccatg agcggcgcac ggcgctgacg ataaattgaa
     1081 aattgctcat cgcttcggtg gaaaccccga ggcgccgata gccgtcctgc atcaggagaa
     1141 agtcttcggg ctggcccaga ggcacggcgt cgcactcgtt tttcttgaga catgcggagc
     1201 gcgccggcgt cccgaccaac tctttgactt tgaaatctcc ttcctcgatt gcatgtaagg
     1261 ccatgacttt gcgcgtcgaa atcgaaatgg tatcgactgc cagagaaaga ccgacggttt
     1321 tgcctttgag atcggcgaag tccttaattt ctaacctggt gattaggctg tagatcggag
     1381 ttccggtttc gccgaacacc gcgacattcc gagccgttca ggacgtcttg gatcaaataa
     1441 ggggtggcgg tacgcgtaat atctacgact cgtttcgata gcgcgagcac catgttggcc
     1501 gcacccccat cgatggggag accttccagg gcgatgccct ctcgagcgaa gaagcccttg
     1561 cgctcggcga gatgaaactg caacgcggat acggttttga gggtggaggg gatttgacca
     1621 tagcgcaacg taataccgtg aacgtgatga gggactgcga aaaaaatgct cccggcgagc
     1681 agaaatgtga atggctgcat cgaaaacctc ctcgtcgggt cgctcaaaac tgcgcgctga
     1741 tagctcagcc cgcgcctttt gtccatgaga tctggctcag gttttagcat ttcggcgctg
     1801 catcggcacc gacgatctgc tataagtagc ggatgcaaat tctccaggtg acgctctgga
     1861 tttacggcat ggcgctgaag cgcgcctggg agtgcgtgcg caaaaatctc gtcgtgagct
     1921 ttgctccgtt ggtgtacagc ctgctgcttt cagccgcggc gattatagct ctgcctttgg
     1981 gcatcctcgg tgggttggta gtcggcctcg tcgcccaagc ctgcctcagc tcgggcctgt
     2041 ttctcgtaaa gaatatgatc gacagcggca aaacggattt gaacgacttt gcgcgcggtt
     2101 tcagggttta tatttgggag ttgatcacga tctccttcat gctctggatt cctttgaagc
     2161 tcgccggtat ggcactcgca acggttccca atggcgggtt gatctatttt tgcattcaga
     2221 tcgccctttt cgttttgctc aatccggtgc ccgaatttat ttaccgtagt cgtacgtcag
     2281 gcccggagct gttgagcgcg agctacaatt ttatcgtgga gaactgggtc gaatggctga
     2341 cgccgaatat tgttctcgcc gtcatcggtt atgtgctact gcaccagatc gaattggcat
     2401 tgtttagttt accggctttc gtccaacttt tcgtacactc cttcgccctt ggtctatttc
     2461 ttacctacgt gatgaccttc cgcgggtttc tgtttattga attatacggc agcagccggc
     2521 gcagtcgaat ttatcgttac aaggcgcgca gcgattaaac gacctcgcgg cgaattcgag
     2581 taacctagga cctggatgcc gcggccagat gacaacacca cgcttggcgt agctaaaatg
     2641 agaaattcga ttccaatagc cgacgagtag agatagcgaa gaaccattga tttcccccgg
     2701 ttaacttgac gtgtgcggct tcttgtggtg tcctgataaa ctcactcgac accatgaaga
     2761 ttaccagcat cagaggattt tccgacatcc tgccggaaga agtcggcgcc tggcagcggg
     2821 tggaaaccgt ggcccggcgt gtgttcgaga tttacaactt taccgaaatc cgtatcccga
     2881 tcgtggaaaa gaccgagctg tttagtcgct ccatcggcga aaccacggat attgtcgaaa
     2941 aggagatgta cacattcgag gatcgcgatg gccgcagtgc cgagaactcc gaagcgatga
     3001 aactcactct gcggcccgag ggaaccgccg gcgtggtgcg ggcatacgtg gaagccgaaa
     3061 tgtacaaatc ggagccggcg cgcaagctct attatatggg gccgatgttt cggcgcgagc
     3121 ggccgcaaaa aggtcgtatg cgccagtttc atcagatcgg cgcggaagct ttgggacgcg
     3181 cggatccgtt catcgatgca gagattttat tattgctcca tgacttgttc gcagcgctta
     3241 aattgactga cttgtctctc gagctcaatt ctctcggcga tacggagtgc cggccacgct
     3301 atcgcgacga gctgctcgcc tttcttaagg cacgggaaga ctcgttgtgt ggcaactgcc
     3361 gccgacgcat cgatcgtaat ccgctacggg cgctcgattg caaggagccg ggctgtatcg
     3421 tggcaacaaa agatgcgccg tccattctcg aagcgctctg cgtgctatgc cgcacgcact
     3481 tcgataccgt gcagaaactg ctggatgaaa ccggcgtgcg atatagcctc aatccgcgta
     3541 tggtgcgcgg gttagattat tattgccgca cgactttcga atggacaacg acgctgctgg
     3601 gttcccagag tgcggtcgcg gcgggaggac gttatgacgg tttagtggaa cagctcggcg
     3661 gcccgccgat tcccggggtg ggctttgcca tgggcgtgga gcgactcacg atgctgctca
     3721 agatgcaaga ggaggggatc caacccggtc ccgatcttta tcttgtctgg gtcggcgaga
     3781 aagcgcgcaa ctgggctttt cccgtcgcgc acacactgcg gcaaagaggc ctggtggtcg
     3841 aaatggaagg cgagagccgc agcttgaaga gtcagatgcg gcgcgccgac aaattcagag
     3901 ccgcatccgt tctgatcgtc ggcgatgatg agcttgccaa gggtgaaggc gtgctgcgtg
     3961 atatggcgag taagcggcag gagaacatca aattggcaaa catcgaggcg gaattgagcg
     4021 cgaggaagtc aggttaatgg ccgaacgcgt ttttaacgat cctttgggcg attggaagcg
     4081 cagttgttat tgcggtgagc cgcgcgcgca agcggtcggc gaagagaaaa tcctcgtcgg
     4141 ctgggtgcac agccggcgcg atcacggtgg ggtgattttc gtcgatctgc gcgaccggac
     4201 cgggatctgc caggtggttt ttcggccgga agtcgccgcg tcaagccatg aaaaagcgaa
     4261 acagattcgc tccgaggacg tcatcgcggt gcgcggcgtg ctcgcgaaaa ggtctccgga
     4321 aagcatcaac cccgagctcg ccacaggcga ggttgagctg gtctgtcgcg agctgcgtct
     4381 gctgaatgcc tcggcggtgc cgccctttcc gatcgaggat gagagcgatg ctgcggaaaa
     4441 cacgcggctc aagtatcgct atctcgatct gcgccggccg cagagtctga gtcctctctt
     4501 gctccgttac cggatgacca agctgatccg cgactatctc gatggattgg gattcattga
     4561 tgtggaaacg ccggtgttga ctaagagcac accggaagga gcgcgtgatt atttggttcc
     4621 cagccggatc tatcacggca aattctacgc gttgccccaa tcgccgcagc tatttaaaca
     4681 gattttgatg gttggcggcc tcgaccgtta ttttcaaatc gtcaaatgct ttcgcgatga
     4741 agatttacgc gccgatcgtc agccggagtt cacccagctc gacatggaaa tgtcgtttgt
     4801 ccaaccggaa gatgtcatcg aggtcgtaga gggcatgatg gttcttctgt tcaaagagct
     4861 gaaaggcgtc gagctgaagc ggccgtttaa aaaattgacc tatgcagaag cgatgaatcg
     4921 ttttggttcg gacaagccgg atatgcgctt tggtttggag atcaaagatt ttactgaggc
     4981 gttacggaac tcgcaggcac gggttttcgc ttcggcgatc aaggcaggcg gagtcgtaaa
     5041 gggcatgcgc attcccaaag gggctgagct atcacgcaaa gacctggacg acatgacgcc
     5101 ttatgtagcg acgtttggcg cgaagggcat cgcctggacg aaactcaccg aagaaggctg
     5161 gcaatcgccg attgcgaaat ttctgtcgga cgcggagcaa aaagacatcg aacgcagcgc
     5221 cggagccgag agcggcgatg tcgtcttgtt ttctgccgat caggcgaagg tcgttcatga
     5281 cgccctcggt aacttgcgct tatatctggg tgagaagttg gatttgattc cgcaaaatga
     5341 atacggcctg gtgtgggtcg tcgattttcc gcttatggaa ttcgatgccg aacagaagcg
     5401 ctacgtcgcc ttgcatcatc cctttacggc gccgctggac gaagacctgc cgttgctcga
     5461 gagcgagccg gcgaagagcc gctcgaaggc ttacgacctc gcgctgaatg gcattgagat
     5521 cggcgggggt agtattcgca tccatcaagt ggaattgcaa aagaagattc tcgctctcat
     5581 gggcatcagc gccgaggaag cgcaggcaaa gtttggcttt ttgctcgaag cgctgagcta
     5641 cggcgcaccg ccccacggcg gcatcgcctt tggcatcgac cgcttggcga tgttgctgag
     5701 cggcgcgaaa tcgctgcgtg atgtaatcgc gtttccaaaa agtcagcgcg cggtctgtat
     5761 gttgacggat gcgccgtcga ccgtggagcc gcgccaattg cgcgagctgg gaatccggtt
     5821 aagcgggggg gagaagtagc aggtgtcagt taccagttgg caggaacagc tgtttcctcg
     5881 agtcagatgg ttgacccgct atccccgaat aactgtgcga ctgtcctgta aggctccgat
     5941 gttaaaattt acgtgcatgg ctttgttgct gctggcgagc tgcagttttg ggccgggaaa
     6001 tctccaaagc cgtcgtgcga cggacttgga atcccgccgt attcgccgca ttgccgtgtt
     6061 accgccaaac gaggaaacag cgcctcggcc acgagccatt cccggaacca cggagccgag
     6121 aaatccggag cgtgacgcgc cggaaacatt gacgcggatt atttattccg tcatggctgc
     6181 gatgcctaat tggcaaattg tctcggacaa tgaggtgcgc gagatcagcc agggcgtggc
     6241 agccacgggc gataccgcgc gtttgcgccg gatcggagaa atggtctatg ccgacgcggt
     6301 gctcgtcagc catgtgcgcc gttatcgcga gagggttggc gacgagtggg gtgccaagag
     6361 cccagcctcg gtggcatttg tcttggatct catcgatgtc agacgcggcg atatcgtgtg
     6421 gtcggcaaat tttgacgaaa cacaaaaatc cctcagcgaa aatattttcg ctctcgggga
     6481 catcacgcgg cgcggcgtcc gctggctcag cgcagatcaa ctcgctcagg aaggggttaa
     6541 aaaagccgtc gcgcagcttc atgacatgat cgcgcgggcg cccgcctcgt cataaattca
     6601 cgagggcccc ggcacctcga tttccaaagg tctcgaaaga agctcgctag cgagcctgcc
     6661 cagttcttta agtttcctga cactgccgcg atccaacaat acggccgcgc catcgcgcaa
     6721 aatcatgcgc agcgcgggca ccgaaaactt ttgcgccgcc gtcgacccgc ccagagcgaa
     6781 gccgctataa acttgatcga agccgagctt gaacttgtca atttcatcgt aggatgcgct
     6841 gagaacaatc gaacggtaca agctatagcg tcggaggatc actcggcgcg cccgagaatc
     6901 gacttcgata acccgccgct gtgtgacaag aaagagtgcg atagcaaaca gaattaaatt
     6961 catcagcggg aaataccaga gcgccccggc aggcccagtg aagccgcgca ggcggagcgc
     7021 ttcgacgata ggtagcgaga caaaaagcaa gagtaccgcg atgcccaggc aaagaaacgc
     7081 gggaaagcgc gccccgtgcc atactagacg gagcttctcc cgtgtgtttt ctacgatctc
     7141 gaaaccgagg cgattgccga tcgtctcaaa ttgcgcggga tcaccgatca tcagcgcagt
     7201 gacagcttat ggtcggccgc tggcgcagcg aagcttcacg acttagcggt tttttgctcg
     7261 cgataaaaat cacgaaaacg atattgcttc agttgaatga ggttttggta ggatccaaag
     7321 cgttccttag gatcattgac ctcatcgagc agatcgcggt gctgcccttc agactcggat
     7381 acacgaattt tttggtaggt ggttgagcgc tcggccggaa tacggctcat ttcacgaatc
     7441 atgccgcgaa attgggacgg cttcatcagt tgtccgtaag aagcaccggc tgcagtcgag
     7501 atgctctcgt tgatcagtgt gccgccgaag tcattggctc cggcaagaag cgcgatctgc
     7561 gaaagcttcg gtccttcctt gacccaggag acctgcagat tgggaatata attgttcagc
     7621 atcagccgcg acaccgcata cattttcatg acctcagcgc cgcttgcgcc gcgtcgcagg
     7681 ccatccggag gcttgcgatg aaacatgggc gcttcttcgt aaacaaagct cagcggtacg
     7741 aactcggtga tgccgccggt gcgtctttga atttgccgca agatgtccaa gtgaaccgac
     7801 ttgtcgcgtg agctttcaac aaaaccatac attatcgtcg aggtggtagg gatgccgact
     7861 tcatgagcgg tttggatgag ctcgatccac tgtgctgggg cgatgcggcc gggtgagacc
     7921 cgctggcgaa tcgcatcgac gagaatttcc gcggaggttc ccggcaagct cccgacgccg
     7981 gcttctttga gcgcgatcag gtagtcgcgg acactgac
//
LOCUS       DASULZ010000570         3374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_878274, whole genome shotgun sequence.
ACCESSION   DASULZ010000570 DASULZ010000000
VERSION     DASULZ010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878274"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VFY96_11735"
     CDS             <1..597
                     /locus_tag="VFY96_11735"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5607182.1"
                     /translation="YVSGIEFFWGYMLSVLVAIPFGIAIGWYKRFAYICDPFVNAMNA
                     TPRIALLPLVIIWLGIGIISKVGIIFLGAVFPLLINTRDGVKTTPHNLLTAARSFGAS
                     DWQIFRSVVLPSTVPFILTGLRLAVGRALIGVMVGELYAATAGIGFMITVAGATFQTD
                     KVFVGVLIFAVSGMTLTAVIDHYEHRFDKWRPKVGAAS"
     gene            complement(1224..1481)
                     /locus_tag="VFY96_11740"
     CDS             complement(1224..1481)
                     /locus_tag="VFY96_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607183.1"
                     /translation="MDFEKAKLNPAAVFKSPQEVVSIQELSREQKIEILQQWEQDARL
                     MEVAEEESMPGPQAKLLQPIRDALHALNYWPDTEHSSPSKA"
     gene            complement(2102..2326)
                     /locus_tag="VFY96_11745"
     CDS             complement(2102..2326)
                     /locus_tag="VFY96_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3185 domain-containing protein"
                     /protein_id="HEX5607184.1"
                     /translation="MRTIIGLGIILIILGIVSFAYHGIPYTSRENVIDAGSIRTSADT
                     QKTIPMSLLSIGLLIVSGAALVAFGSKNSS"
     gene            complement(2478..3221)
                     /locus_tag="VFY96_11750"
     CDS             complement(2478..3221)
                     /locus_tag="VFY96_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX5607185.1"
                     /translation="MFPFGIVATVFVIVVLLIISGIKILREYQRAVIFRLGRLVSARG
                     PGITYVIPWVEKMIRVELRTVTMDVPPQDVITRDNVSIKVSAVLYFRVVDPNRALTEV
                     QNYLFATSQLAQVTLRSTCGQALLDELLAERDKINTRIQEILDFHTEPWGVKVVLVEV
                     KDIDLPQEMQRAMAKQAEAERERRAKVIHAEGEFQASQKLADAAEMIATQPAAMHLRF
                     LQSLVEVSAENNSTVVVPLPIDLLRRFLP"
BASE COUNT          759 a          874 c          853 g          888 t
ORIGIN      
        1 tacgtcagcg gcatcgaatt tttctgggga tacatgcttt ccgttcttgt cgcgattcct
       61 tttggcatcg cgatcgggtg gtacaagcga ttcgcttaca tttgcgaccc atttgtcaac
      121 gccatgaacg cgaccccacg aatcgctctc ttgcccctcg tcattatttg gttgggtatt
      181 ggcattatct ctaaggtcgg tatcattttc cttggcgctg tcttcccctt gctgatcaac
      241 acccgcgacg gagtgaagac gacacctcat aatctcctta ccgcggcaag aagctttggc
      301 gcttcggatt ggcaaatctt taggtctgtc gtgcttccat ctaccgtacc tttcatcttg
      361 accggtctgc gtttggcggt gggtcgcgcg cttatcggcg tcatggtcgg cgagctctac
      421 gcagcgacgg cggggattgg tttcatgatc accgtcgccg gcgccacctt ccagacggac
      481 aaggtcttcg tcggagttct tattttcgcg gtttcaggaa tgacgctgac cgcagtaata
      541 gatcactacg agcaccggtt tgacaagtgg cgtccaaagg tcggcgcggc ctcgtgatgc
      601 cgaccggttg cagtgaatta ataaaaattt tgcagagcct atgacgctcg gccaaaggag
      661 taaccatcat gagcaaaggc aaggacagta ggaagaacga gaaaaagaaa cccctaaaga
      721 cgttgatgga aaaaagagcc gcgaaaaggg ttaagaaaga gaacaaatcc ttttcgatcg
      781 ttactaaatg atcgatacgg aaaaaggcga cagccagccg aagcctaagt tggatcaggt
      841 tttgcgctag cacatcgtca tattattaga cgcggaagcc ttcgagagtt gcgctctgta
      901 tcttgctgtg ttcatccgta gagtgagtcg attacagtgt caccactcga tcctttagtt
      961 tccctttggc tgctcgttcc aatagtttgt cgattctgct ttgcgtctga tacccgatct
     1021 gctcatggtc gcattcctgg caaactgtgc agggaatgtt acggatctcg agcaggtcag
     1081 tttgtattgt tgtccgaccc aggtaagtct ttgaaccaca ctgtgcgcat tttccaggca
     1141 tgcggtactg tcgctagagg tttgcgggac gagtattcgc cagaaatagt tcttaactcg
     1201 ttcaagctct ggggtaaggg tttttaggct ttgctggggc tcgagtgctc ggtatcgggc
     1261 caatagttga gggcatgtag agcgtcgcga atcggctgga gcagtttggc ttgcggtccg
     1321 ggcatgcttt cttcctccgc aacttccatt agcctcgcgt cctgttccca ttgctgcaag
     1381 atctcgattt tttgttcgcg cgacaattct tggattgaaa caacctcttg agggctctta
     1441 aaaacggctg ccggattcag ctttgctttc tcaaaatcca ttttgctttc ccctactttc
     1501 ttcatgatag cgctgaggca ttgttaagca agcactgtca aaaattttgc tctgtggtgt
     1561 ttgccgagaa ttttatctgg agcagtaaac cggttgcgtt ctcgtgcctg cacgagtcaa
     1621 ttgagtcctt tctttcctac ttctccattt taggcaacgt ggcttttgat tttttctccg
     1681 ccatgtactt tctcaaagca aggcgcctct tttgtctctt acgcctgcgg gaattttttc
     1741 gtgcgtcctc tttcatggtt aatctccttg tgtgctggaa tgaaaccggc ggattgttga
     1801 cagatgagag tttccgcgcc gcgaaggttt agcaccctcc gccgacttta gtccgccctg
     1861 cggaccgctc cgcctctctg ctagcgccaa gcctaatctg gggtcggacc agccatgaag
     1921 aggcaggcgg cacgattaac taaagtcttt caacaataga gcttgaccgc aggccttaac
     1981 tacgattgag tgaatcggcg gcggcgagtg taccgagtcg gacaaaaaca aatcatctca
     2041 aagcgacgcc gaagatcatt ccggatcatc aaggctcttg tcttcgggcc gcgcagtgcc
     2101 actaagacga gtttttcgat ccaaaggcga cgagcgcggc gccactcaca attagcaacc
     2161 ctatcgacag cagtgacatc gggatggtct tctgggtatc ggcactggtt cggatggagc
     2221 cggcatcgat aacgttctcg cggcttgtat agggaattcc gtgataggca aaagacacaa
     2281 tcccgagaat gatgagaatg attccgaggc ctattattgt acgcatggat tattcctttt
     2341 ttacgcctat ttaaaaattt aaagcaacct caaggatgga ccgggcgcct ttaaaatagt
     2401 ttatgtattc gcctccgggg ttgctgtcca ataatggaca gtcttgaaaa gaatattacc
     2461 tgccgaccgt gatggcacta cggcagaaaa cgcctcagca aatcgatcgg gagcggcaca
     2521 actacagtgg aattgttttc cgcggatacc tcaacgagac tttgcaggaa tctgagatgc
     2581 atggccgcag gttgtgtggc aatcatctcc gcggcatcgg cgagcttttg cgaggcctga
     2641 aactcgcctt ctgcatgaat gactttcgca cgccgctctc tctctgcctc ggcttgctta
     2701 gccatggcgc gctgcatctc ctgggggaga tcaatgtcct tgacttcgac caaaaccact
     2761 ttgactcccc agggctcagt atgaaaatcc aggatctcct gaattcgggt attgatcttg
     2821 tctcgctcag ctaaaagctc gtccaataaa gcttgtccgc aagtgctcct cagcgtcacc
     2881 tgagccagct gcgaagtggc gaagagataa ttttgcacct ctgtcagcgc tcgattggga
     2941 tcgaccaccc gaaagtagag aacagcactg actttgatcg agacgttgtc gcgggtgatc
     3001 acgtcttgcg gaggaacatc catcgtcacc gtgcgaagtt ccacccggat cattttttcg
     3061 acccatggaa tgacatacgt gattccggga ccacgcgccg ataccaaacg gccaagcctg
     3121 aagatcaccg cccgctgata ctctctcaag atcttgatcc cgctgatgat caataaaacg
     3181 acgatcacga agacggtggc aactatccca aaaggaaaca tagcgtcccc tcacttatcg
     3241 ctctttttcc atcggtcgcg caaccaccgg ggcattgaag tagtccttac gtgaacgctt
     3301 gttccagctg gcgcaaaatg ccgtcgggtt cacgcaccgc cagtgaaacg atttcatccg
     3361 cacgtgtttg agag
//
LOCUS       DASULZ010000571         5105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1183083, whole genome shotgun sequence.
ACCESSION   DASULZ010000571 DASULZ010000000
VERSION     DASULZ010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183083"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VFY96_11755"
     CDS             <1..470
                     /locus_tag="VFY96_11755"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX5607186.1"
                     /translation="QVLPINYTGLALILLGIGLLVGEAFFPNFGVLGIGGIVSLALGS
                     LLLFDTPLSDFGVDRSIVFTAVGTIGSFVLAISYLVFRSQKTQPALGKEGLIGQIGEV
                     RGKLAPTGRIFVHGENWSAHADAPIDVGEKVRVVGYDGMQLKVRRLSEGDAQS"
     gene            483..1235
                     /locus_tag="VFY96_11760"
     CDS             483..1235
                     /locus_tag="VFY96_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX5607187.1"
                     /translation="MFGFGAPLTFLIIVIALIISGIKILKEYERAVVFRLGRMVGARG
                     PGITYIIPGVEKMVKMDLRTVTMDIPPQDVITRDNVSVKVNAVLYFRVLEPSRAVVEV
                     ENYLFATSQLAQVTLRSVCGQAELDELLAERERINSRIQEILDQQTDPWGIKVVLVEL
                     KYIDLPQEMQRAMAKQAEAERERRAKVIHADGEFQAAQKLADAAEVIGKQPMALQLRF
                     LQSLVEVASEKNSTTIFPVPIDLLTPFLKKNE"
     gene            1323..2363
                     /gene="queA"
                     /locus_tag="VFY96_11765"
     CDS             1323..2363
                     /gene="queA"
                     /locus_tag="VFY96_11765"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEX5607188.1"
                     /translation="MDLKEFDYVLPESLIAAVPSRRREGSLLLVLQRSSGEIVHSCFS
                     ELSDFLSAGDLLVLNDTKVFPARLIGQKESGGRVEVLLLERFPEPNRTLWVALIDAGK
                     KPRVGSELRFGAEITAEVLGDLGRGRFGLEFHHVGNFDEQLQSLGEPPLPPYVRRGRA
                     VEALDHDRYQTVYARHSGAVAAPTAGFHFTDELFETLAAKEIDRTLLTLQVGPGTFQP
                     VRAERIESHRMEGERYSVSAAAAARINHAKESGGRVIAVGSTSTRTLEWVARRHGRIV
                     ADEGITRLYIHPGEQFHVIDGLITNFHLPRSTPLILVAAFAGLELVRRAYREAIEKKY
                     RFYSYGDAMLIL"
     gene            2415..3524
                     /gene="tgt"
                     /locus_tag="VFY96_11770"
     CDS             2415..3524
                     /gene="tgt"
                     /locus_tag="VFY96_11770"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX5607189.1"
                     /translation="MSEHFTILQQDKDTSARVGRLVTAHGEVETPCFMPVGTQGTVKA
                     MLPRDLREIGCQILLGNTYHLYLRPGHEVIRRLGGLHRFMGWNGPLLTDSGGYQVFSL
                     GAMRKLSEEGVNFQSHLDGASHLLTPEKSIEIQQALGSDIIMVLDECVSGDAPREYVQ
                     PSTERTVRWAERSLRAKSAKSQLIFAIVQGGVFEDLRHSCVNALVSMPFDGFAVGGLG
                     VGEGEAELHNIGAFSAKLLPRDRPRYLMGVGRPRDIVQAVRAGFDMFDCVIPTRNTRN
                     GTLYTFTGKLNIKRAEFAEDPRPVEQNCGCYACKNFSRAYLRHLYMAGEILSAQLNTL
                     HNLYFYHRLMDKCREAIRADRADLWPEISPGEETP"
     gene            3553..3888
                     /gene="yajC"
                     /locus_tag="VFY96_11775"
     CDS             3553..3888
                     /gene="yajC"
                     /locus_tag="VFY96_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX5607190.1"
                     /translation="MFDVAYAQAVPGAGGPGPMMTLIPFVLIFVIMYFMVIRPQQKKS
                     KEHQDLLNKLKKNDEVMTSGGIYGKVVDLKETVVTLEVAPNVRIRVHRPQISAVLTGE
                     KSTGKEGKD"
     gene            3892..>5105
                     /gene="secD"
                     /locus_tag="VFY96_11780"
     CDS             3892..>5105
                     /gene="secD"
                     /locus_tag="VFY96_11780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX5607191.1"
                     /translation="MRKSVLTRFAIFGIASLAALLYLLPTFVRSLPEWWSNVLPSDKI
                     HLGLDLQGGSHLVLEVKVDKAIENNVERVRGDLANAFREKGVSGVSVERVQSTLLQIK
                     APAASAERVRGILKSDFAYLVEAKPSQTTASGSEFFLTLSKEEMRSLRDYAVDQSLET
                     IRNRIDQFGVSEPIIQRQGQTNILIQLPGIQDPQRAKEIIGKTALLEFKLVDDTANLE
                     DAIKTGPPPGRQVLYGHAGRGDGISTEKQAYVVESRPLMTGEYIQDARVRPAQQLQGA
                     SVELILNGAGARLFEQITAANVKRRLAIVLDNRVYSAPVIQERIGGGRASITGNFDLK
                     EARDLSIVLRAGALPAPVEIAEERTVGPSLGQDSIHQGMTSFIVGGGLVIVFMIAYYK
                     GAGLLAVAAVIF"
BASE COUNT         1094 a         1310 c         1543 g         1158 t
ORIGIN      
        1 tacaggtact gcccatcaat tacaccgggc tcgcgctcat tttgctgggc attggtttat
       61 tagtcggcga ggcgtttttc cctaatttcg gagtattggg gattggtggg attgtgtcct
      121 tggcgctcgg atcgctcttg ctcttcgata ccccgctttc cgatttcggg gtggatcggt
      181 cgattgtatt taccgcggtc ggcaccatcg ggagctttgt tcttgcgatc agctatttag
      241 ttttccgctc gcagaagacc cagccggcgc tcggcaagga aggtttgatc ggccaaatcg
      301 gtgaagtgcg cggcaagctt gctccgacgg gcagaatttt tgtgcacggt gagaattgga
      361 gcgcacacgc cgatgcgccg atcgatgtcg gcgaaaaagt ccgggttgtc ggttacgacg
      421 gcatgcagct caaggtgcga cgattgtccg aaggtgacgc gcagagctag gaggaagcag
      481 ccatgtttgg attcggtgcg ccccttacgt ttctaatcat tgtcattgct ctgatcatta
      541 gcggcatcaa gattctcaaa gagtatgagc gggcggtggt gtttcggctt ggacggatgg
      601 ttggtgcgag gggccccggt attacgtaca tcattccggg tgtcgaaaaa atggtcaaaa
      661 tggacctgcg cacggtcaca atggacatcc caccgcagga cgttattacg cgcgacaacg
      721 tgtccgtcaa ggtcaatgcc gttttatatt ttcgcgtcct cgagccgagc cgcgcggtcg
      781 tggaagtgga aaattattta tttgcgactt cgcaattggc gcaggtgacc ctgagaagcg
      841 tctgcggcca ggccgaactc gacgaactgc ttgctgaacg ggagcgaatc aatagtcgca
      901 ttcaagaaat cctcgatcag cagaccgacc cttggggcat caaagttgtc ctggtcgagc
      961 tgaagtacat cgacctgccc caagagatgc agcgggcgat ggccaaacaa gccgaagccg
     1021 agcgcgagcg gcgcgccaag gtaatccatg ccgacggcga atttcaagca gcgcagaaac
     1081 ttgcggatgc ggccgaagtc atcggcaagc aaccgatggc gctgcagctg cgatttctgc
     1141 agagtctcgt cgaagttgcc tcagaaaaga actcgaccac gatttttcca gtgcccattg
     1201 acctgcttac gccgtttttg aaaaaaaacg aatagccact aagctggcaa tactctcacg
     1261 agcttttata gtgcttacga gtggggccgg aggaagccat tccatccctg tcgttttcgt
     1321 ctatggacct gaaggagttc gattatgtct tgccggagtc gttaattgcg gcggttccaa
     1381 gccgccggcg cgagggttct ctgcttctcg tgctgcaacg gagctccggc gaaattgttc
     1441 acagctgttt ttcagagctc agcgatttcc tcagtgccgg agatctcctg gtcttaaatg
     1501 atacgaaagt ttttccggcg cggctcatcg ggcagaagga aagtggtggt cgcgtcgaag
     1561 tcttgctgtt ggaacgcttt cctgaaccta atcgaaccct ctgggttgca ttgatcgatg
     1621 caggcaagaa accgcgggtc gggagcgaat tgcgttttgg cgccgagatc accgctgaag
     1681 tcctgggtga tcttggacgc ggccgctttg ggcttgagtt tcatcacgtc ggcaatttcg
     1741 atgagcaact ccaatcactc ggggagccgc cgctgccgcc gtatgttcgc cgaggccgcg
     1801 cggttgaagc cttggatcat gaccgttatc agacggtcta tgctcgccat tccggtgcag
     1861 tggcagcacc gaccgccggg tttcatttta ccgacgagct gttcgaaaca ttggccgcga
     1921 aagaaattga ccgaaccctc ttaacccttc aggtgggccc gggtacgttc cagcccgtgc
     1981 gcgctgagcg catcgagagt caccgcatgg aaggtgagcg ctacagcgtg agcgcagcgg
     2041 ccgcagcgcg gatcaatcac gcaaaagaga gcggcggccg cgttatagcg gtcgggtcga
     2101 cgagcacgcg cacgctcgaa tgggtggcgc gccgccatgg tcgcatcgtg gcggatgaag
     2161 gcatcacgcg tctttatatt cacccggggg aacagttcca cgtcatcgac ggcttgatca
     2221 ccaatttcca tttacctaga tcgacgccgc tgatcctggt cgccgcattt gcgggtctgg
     2281 agctcgtccg acgtgcctac cgcgaagcga tcgagaagaa gtatcggttt tatagttatg
     2341 gcgatgccat gctgatcctg taggcgagta gcgcaggctt aggcatcagt ttcgatcctt
     2401 ttgagttttc tttcatgagc gagcatttca cgatcttaca gcaggacaag gataccagcg
     2461 cgcgagtcgg ccggctcgtc actgcccacg gcgaagtcga gacgccatgc tttatgccgg
     2521 tggggacgca gggaaccgtt aaagccatgc tgccacggga tttgcgcgaa atcggctgtc
     2581 agattttatt gggtaatact tatcacctat acctgcggcc cggtcacgaa gtgattcgcc
     2641 gtctcggggg gctgcaccgt ttcatggggt ggaacggccc gctcttgacc gacagcggcg
     2701 gctaccaggt gtttagtctc ggcgcgatgc gcaagctcag cgaagagggt gtcaattttc
     2761 aatcgcatct cgatggcgcg agtcatttgt tgacgccgga gaaaagcatt gagattcaac
     2821 aggcgctggg gagcgatatt attatggtgc ttgacgagtg cgtttccggc gatgcgccgc
     2881 gggaatacgt tcagccctcc accgagcgga ccgtgcgttg ggcggagcgg tctttgcgag
     2941 caaagagtgc aaaaagtcaa ttgatatttg caattgtgca gggtggcgtg ttcgaagatt
     3001 tgcgccattc gtgcgtgaac gcgcttgtct cgatgccgtt cgatggcttc gccgttggcg
     3061 gtttgggtgt cggtgaaggc gaggcagagc tccacaatat cggcgcgttc tccgcgaaat
     3121 tacttccccg cgatcgaccg cgctatttga tgggagtggg ccgcccacgg gatattgtgc
     3181 aggcggtgcg cgccggtttc gacatgttcg actgtgtgat tccgacgcgc aacactcgca
     3241 atggcacgct ctatactttt actggcaagc tcaacatcaa gcgcgccgag tttgccgagg
     3301 atccacgccc agttgagcag aactgcggct gctatgcctg caaaaatttt tctcgcgctt
     3361 acttgcgcca cctttacatg gcgggggaaa tcctctcggc gcagctcaac actcttcata
     3421 atctttactt ttaccatcgg ctcatggata agtgtcgaga ggcgattcga gctgatcggg
     3481 ccgatctttg gccggaaatt tcaccagggg aggagacacc ctgacgcgaa atcgcacctt
     3541 gtaggaggaa agatgttcga cgttgcatat gcgcaggcgg tgccgggcgc cggcggtccg
     3601 gggccgatga tgacgctcat tcccttcgtt ttgatctttg tcatcatgta tttcatggtc
     3661 atccgcccgc agcagaaaaa aagtaaagag caccaggatc tgttgaacaa attgaaaaag
     3721 aacgacgagg tgatgacgtc cgggggaatt tacggtaagg tggtcgacct aaaggaaaca
     3781 gttgtgacgt tggaggtggc gccaaacgtg agaattcgcg tgcatcgacc acagatcagc
     3841 gcggttctta ccggggagaa aagcaccggg aaggaaggga aggattagcg aatgcgcaaa
     3901 agtgttttaa cgcggttcgc catattcggc atcgccagct tggcggctct gttatatctg
     3961 ctccccacct ttgtgagatc gcttcctgag tggtggagta acgtcctgcc cagcgacaag
     4021 atccatttgg gtctcgacct tcagggcggg agccatttgg ttctcgaagt gaaggtggac
     4081 aaagcgattg aaaataatgt cgagcgggtg agaggagatt tggcgaacgc gtttcgggag
     4141 aagggagtat ccggagtttc agttgagcgc gttcagagca cgttgctaca gatcaaagcg
     4201 ccggctgcaa gcgcggaacg ggtccgcggg attttaaaaa gcgattttgc ctatctcgtg
     4261 gaagccaagc cgtcacagac gactgcaagc gggagcgaat tcttcctgac gttaagcaag
     4321 gaagaaatgc gttcgctgcg tgattatgcc gtcgaccaat cgctggagac cattcgcaac
     4381 cgaatcgatc agttcggcgt cagcgaaccg attatccagc ggcaaggcca gaccaatatc
     4441 ctcatccagc ttcccggtat tcaagatcca cagcgtgcca aggagatcat cggcaaaacg
     4501 gcgcttttgg aattcaagct cgtcgatgac acggcgaacc tcgaagatgc tatcaagacc
     4561 ggtccaccgc ccggccgaca ggtgctttac gggcatgcgg ggaggggcga cggtatcagc
     4621 actgaaaagc aagcgtatgt cgtggagtcg cgccccttaa tgaccggtga atacattcaa
     4681 gatgctcgcg ttcgcccggc gcagcagctg caaggtgcgt cggtggagct aattttaaac
     4741 ggcgcgggcg cgcggctatt tgagcagatt accgcggcca atgttaagcg gcggctcgcg
     4801 atcgtgctgg acaaccgggt ctattctgcg ccagtcattc aggagcgaat tggcgggggc
     4861 cgcgcatcca tcaccggtaa tttcgacctc aaggaggcca gagacttatc cattgtactc
     4921 cgagcgggcg cattgcctgc gccggttgaa atcgcggagg aaagaaccgt tggcccttcg
     4981 cttggacagg attcgattca ccaaggaatg acctcattta tagtcggcgg cggactggtg
     5041 attgtgttca tgatcgccta ctacaaaggc gcgggattgc tggcggtggc agcggtcatt
     5101 tttaa
//
LOCUS       DASULZ010000572         4962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_472412, whole genome shotgun sequence.
ACCESSION   DASULZ010000572 DASULZ010000000
VERSION     DASULZ010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472412"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VFY96_11785"
     CDS             complement(<1..181)
                     /locus_tag="VFY96_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX5607192.1"
                     /translation="MQLPKTYSHKDIEAKWYNHWMSRGLFAPASRSGPSFSMVIPPPN
                     VTGSLHMGHALNNSLQ"
     gene            complement(207..677)
                     /locus_tag="VFY96_11790"
     CDS             complement(207..677)
                     /locus_tag="VFY96_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607193.1"
                     /translation="MNRILVIEPFQMLRHAFAVALSSEYQVETLADFPDESLLDRADL
                     VVVNAATLRTSEKLDGNKLDMVGAWKKPVIWIDTDGGAEPNAFSQCCRLTWPLDRDGL
                     RKAIASCLHASPKEIVQQKKATPPGSLKVQHSENRAASTDLGEKRLIELVDIVE"
     gene            751..837
                     /locus_tag="VFY96_11795"
     tRNA            751..837
                     /locus_tag="VFY96_11795"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:785..787,aa:Leu,seq:cag)
     gene            complement(1349..2044)
                     /locus_tag="VFY96_11800"
     CDS             complement(1349..2044)
                     /locus_tag="VFY96_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017841866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEX5607194.1"
                     /translation="MEFTQFMLRWVHFLAGITWIGILYYFNFVQTPFFAETEAGVRTA
                     AIQKLVPRALWWFRWGAMFTFLAGISIYLMRMSEMGAGLFYSSPYGVTITVGGLLGTL
                     MFLNVWLVIWPNQQVVIASANQVASGGQALPAAAGAGRRGALASRTNTVLSIPMLLYM
                     GAASHYPQMVHQSTSRGLFWLLVIIIVGAVEANALVGTQGATKVPLDSVNGALWFGFL
                     LAGILFLLTIGLT"
     gene            2246..3346
                     /locus_tag="VFY96_11805"
     CDS             2246..3346
                     /locus_tag="VFY96_11805"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX5607195.1"
                     /translation="MTNPVIYAIFPAAFWPARGVSLIQKTKSHRMPADVSSGRERRIP
                     LLHGSKDPFACVSEALERVEENLARAIRSREQDLTDISAHLILSGGKRVRPMVTLLAY
                     SAFGGQHTQDIVDIATAIELIHTATLLHDDIIDGAETRRGKKSAYKKFGLKSTLVAGD
                     FLFIKAFEFAGKFDETVVQWTADACTLLTEGEILQGHFNRNPAVTLDDYLEIVKRKTA
                     SLFHTGAKVGAYLAGAPTALVEEAERYGLNMGIAFQMVDDVLDVVGHVDLLGKPVGMD
                     LRDGNPALPIILAVNNQEPAVSAVFENSKPSEEQIDSALSAIRTGSSIQEAKSLSKRY
                     AREALKSVKKLPPSMYRNGLKTLVQLIIDRDF"
     gene            3385..4113
                     /locus_tag="VFY96_11810"
     CDS             3385..4113
                     /locus_tag="VFY96_11810"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HEX5607196.1"
                     /translation="MSAIDDSRDRSVRSMFDRIAGRYDLLNRLISFRLDAYWRNQAIR
                     AAVTGAHPHILDLGTGTGDLALNAARQALGNGRIVGLDFSLPMLRLAHAKRAKAMYGD
                     GATFVMGNAMSAPFKNAIFDSAMTAFVLRNVSDLPLMFTEALRVLKPGGKFVSIDMFP
                     PAASWFARLYSIYFYRVMPRIGGFLSHDQKAYQYLSDSVRQFHSPEQVSQFLQAAGFR
                     QVCLRKFFGGAVCMHVAVKPGTAF"
     gene            4123..4953
                     /locus_tag="VFY96_11815"
     CDS             4123..4953
                     /locus_tag="VFY96_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607197.1"
                     /translation="MQGFSNSFLVFLYQIAFGGLVAVAGTPFHEIERAFYKSTAGVLY
                     VIALLQLWGESSLLMQGTRQASLWGNRLEIIVHLMFVLAFSCYMASLWIDLPAFRARS
                     FATSILIGLLGLMLSAQRFYDAPFFSLETLVYPAIFFVSALLLGSVTVGMLIGHWYLI
                     DTGQSLDPFLRIYKFFVFSLIAQCVLFALFPVLLHFFGVASTVNAMEQLWRQHSTLLA
                     TRVIIGQCAPLVLAWMIWRTLLIPHTMAATGLFYIALLGVFVGEILGRQIIALTALPF
                     "
BASE COUNT         1096 a         1358 c         1268 g         1240 t
ORIGIN      
        1 cttgcagcga attgttcagg gcgtgcccca tatggagcga gccggtcaca ttcggaggcg
       61 ggatgaccat tgaaaacgat ggcccggatc ggctagcggg cgcaaacaac ccgcggctca
      121 tccagtggtt gtaccatttc gcctctatat ctttgtgact gtaagtcttc ggcagttgca
      181 taatcaggcg acgttaagaa aattatctat tcaacgatgt ccaccaactc gatcaatctt
      241 ttttcgccaa gatccgtcga ggcggcgcga ttttcagaat gctgcacctt cagtgatcca
      301 ggtggtgtgg ctttcttttg ctggacgatt tctttcggac ttgcatgcaa gcaacttgcg
      361 atggcttttc ttaaaccatc tctgtcgagc ggccacgtca atcggcaaca ctgggagaat
      421 gcgttcggct ctgcgcctcc gtccgtatcg atccaaatta ccggtttttt ccaagcaccc
      481 accatgtcca gcttattccc atcgagcttc tcagaagttc tcaaggtcgc cgcattaacg
      541 accacaagat cagccctgtc taataaactt tcatcgggaa aatcggccag ggtttccact
      601 tgatactcag aagaaagtgc gacggcaaag gcgtgacgca gcatctggaa gggttcgata
      661 actaagattc tgttcataga cttatccgta gcaaaacttg acaaggctta ggacccaagt
      721 tacaaaaact gaagctgatg cagcaccttc gcgaaagtgg cggaattggt agacgcgcag
      781 gattcaggat cctgtgggat aacacccgtg ggggttcgat tccccccttt cgcaccaacc
      841 ggttcacaca tatcacacaa cgctgagttt ctctattgat cgcgaccatt ccgcagagct
      901 aagcctgtca ggtgataatc tccagaaccg gtcggccggg ccacaagtat ttttttcgaa
      961 tggccacgga ctcgagcgaa aaaattcgct ccggcagcag cgacagggtt gcctcatgaa
     1021 acttctattt tgcttcgcaa tactagctgg attggctctg ggcgcttgtt cgaaagaatc
     1081 gaatgattcg ccgaagagcg ccgattggca aagaggcagg gccgtttttc tcgcaaactg
     1141 tgttgcgtgc cacaatagcg atccgtcgaa agacggaccg atcggccctg cgatcagggg
     1201 atcatcgaga gaactgctcg aagcgcgggt gctaagtaca tcctatccgc ccaattacaa
     1261 accgaagcgg ccaacgaagg tgatgcctca gtttccgttt ctaaaagacg aaatcccgta
     1321 cttagctgcg tttcttcagc aggatcggtt aagtcaatcc gatcgtcagc aaaaacagaa
     1381 tcccggccag taaaaagccg aaccaaagag cgccattcac tgaatcgagc ggcaccttgg
     1441 tcgcgccttg tgtgccgacc agagcattag cttccactgc accgacgata ataatcacca
     1501 gcaaccagaa caatcctcgt gaggtggatt gatgcaccat ctgcggatag tgactggccg
     1561 cgcccatgta caacaacatg ggaatcgaca aaaccgtgtt ggttcttgat gccagagccc
     1621 cgcgacggcc tgccccagcg gctgctggga gcgcctgtcc acccgaagcg acctggttgg
     1681 ctgaggcaat caccacctgc tgattgggcc aaatcacgag ccagacattg aggaacatca
     1741 gtgtgccgag caaaccgcca acggtaatcg tcacaccgta aggtgacgag taaaatagcc
     1801 ccgcacccat ctcgctcatt cgcatgagat agatcgagat gccggcgagg aacgtgaaca
     1861 tggcgcccca acgaaaccac catagcgctc gcggcacgag tttttgtatc gccgcggttc
     1921 tcacgcccgc ttccgtttcg gcaaaaaagg gcgtctgcac aaagttgaag taataaagaa
     1981 taccgatcca agtaatacct gctaaaaaat gcacccaccg caacataaac tgagtaaact
     2041 ccatgttcct cgcctccttt agtgcgctgt attctacggt gctaaccgaa tcctaagtct
     2101 tgcttcgggg cctatatagt cgggctctct cgtttttgtc aagataaatg tcacttgtcg
     2161 agcgcgcacc tgtttttgtg cttcgcagct ctcgtgcgtc tcagtccgcg cgtatcaatg
     2221 cagcaccagc atcgcagcat tcggcttgac caatccggtg atctatgcca tttttcctgc
     2281 agctttctgg cctgcgcgcg gagtctctct gatccaaaaa accaaatcgc atcggatgcc
     2341 ggccgatgtc tcgtccgggc gggagcgaag aatacctttg ctgcatggca gcaaagatcc
     2401 cttcgcctgc gtcagcgagg cacttgaacg ggtcgaagaa aatctcgcgc gcgcgattag
     2461 atctcgagag caagacctta ccgatatttc agcgcacctc atactgagcg gcggcaagcg
     2521 agtgcgtccg atggtcacgt tgttggctta ctcggccttc ggcggtcaac acactcagga
     2581 catcgtcgac atcgccacag cgatcgagct gatccacacg gcgactctgt tacacgatga
     2641 tattattgac ggcgctgaga cgcggcgggg caagaaatcc gcatacaaaa aattcggctt
     2701 gaagtccact ctggtcgctg gagatttcct gtttatcaaa gcttttgaat ttgccggcaa
     2761 gttcgatgaa accgtggtgc aatggaccgc cgacgcctgc acgctcctga cggagggaga
     2821 aattctccaa ggccacttca atcgcaaccc ggccgtaacc ctggacgatt atctggaaat
     2881 cgtcaaacgc aaaaccgcgt cgctgtttca taccggcgcc aaagtcggcg cgtatctagc
     2941 cggagcgcca acagcgctgg tcgaagaggc cgagcggtac ggcctgaaca tgggcattgc
     3001 gttccagatg gtcgacgacg ttctcgatgt cgttggccat gtggatctgt tgggtaagcc
     3061 ggttggcatg gacttgcgcg atggcaatcc cgcattgccg attattctcg ccgtgaacaa
     3121 ccaggaacct gcggtcagcg cggtatttga aaacagcaaa ccttcggagg agcagatcga
     3181 cagcgcactg tccgcgatca ggacaggctc ctccattcag gaggctaagt cgctttcgaa
     3241 gcgctacgcc agggaagcct tgaaatcggt aaagaaactg ccgccttcta tgtatcgcaa
     3301 cggcctgaag actctcgtgc agttaattat cgatcgagat ttttagttcc ataccgctgg
     3361 agtaactcgc tcgtttctcc cttcatgtcc gctattgatg attcgcgcga ccgatccgtt
     3421 cggagcatgt tcgatcggat cgccggacgt tatgatttgc ttaaccgtct aatcagtttt
     3481 cgtcttgatg cgtactggag aaatcaggcg atacgcgccg cagtaacggg cgcccatcct
     3541 cacattctag atctcggcac agggaccggt gacctcgctt tgaacgccgc ccggcaggcg
     3601 ctcggcaatg gacgaattgt cggtttggat ttttcgctcc cgatgctccg tttggcccac
     3661 gccaaacgcg ccaaagcaat gtacggcgac ggcgctacgt ttgtcatggg caacgcgatg
     3721 tctgccccct tcaaaaacgc gatcttcgat tctgccatga ccgcttttgt gctgcgcaac
     3781 gtttctgacc tgccgctaat gttcactgaa gcgctccgcg tcctaaagcc cggcggcaaa
     3841 ttcgtttcca tcgatatgtt tccgcctgcc gctagctggt tcgcacggct ttactcgatt
     3901 tatttttatc gtgtaatgcc gcgcatcggt ggctttttat cgcacgatca gaaagcctat
     3961 cagtatcttt ccgattccgt tcggcagttt cattcgccgg agcaggttag tcaatttctg
     4021 caggcggccg gttttcgcca ggtctgccta cggaaattct tcggtggtgc agtctgcatg
     4081 catgtcgcag tcaagcctgg cacagcgttt tgagcaaact ctatgcaagg tttctccaat
     4141 agttttctgg tgtttcttta ccagatcgcc ttcggcggac ttgtcgcggt cgcaggaacg
     4201 ccttttcacg aaattgaacg agctttttac aagtccaccg caggtgtgct ttacgtaata
     4261 gctttacttc aattgtgggg cgaaagttct cttttaatgc aaggcacgcg gcaagcatcg
     4321 ctgtggggaa atcgtctcga aatcattgtt catctgatgt tcgtgctcgc gttttcctgc
     4381 tatatggctt ccctctggat cgacttgccg gcttttcgcg cacggagctt tgcgacttct
     4441 atcttgatcg gactgttggg cttgatgctg agcgcgcagc gtttttacga cgcgcctttt
     4501 ttctcgcttg agacattggt ttaccctgct atttttttcg tctcagctct gctgctcggc
     4561 agcgtaacgg tcgggatgct gattggccac tggtatctta tcgatacggg gcaaagtctt
     4621 gatccttttc ttagaattta caaatttttt gttttctcgc tgatcgcgca gtgcgtctta
     4681 tttgcgttat tccctgtgct tctgcacttt tttggtgtcg cttctaccgt gaacgcgatg
     4741 gagcaactat ggcggcaaca ttcgacgttg ctggcaaccc gagtcatcat tgggcaatgc
     4801 gcgccgttgg ttttggcatg gatgatctgg cgcacgctcc tcatcccaca taccatggct
     4861 gctactgggt tgttctatat cgcgcttctg ggagtttttg tcggagagat tctcggacgg
     4921 cagattatcg cgctaacggc gttgcccttc tgatttctcc ac
//
LOCUS       DASULZ010000573         2614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_67543, whole genome shotgun sequence.
ACCESSION   DASULZ010000573 DASULZ010000000
VERSION     DASULZ010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67543"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VFY96_11820"
     CDS             <1..663
                     /locus_tag="VFY96_11820"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5607198.1"
                     /translation="LTVFEELRNDDTIAVVLTTGAGDVAYCAGRDLSEFPTEGGKNRG
                     KDRRAEPRAYHVAELIRTFPKVTVAVVNGFCLGAGITLLLPHDLAIASDRAQFGLPEI
                     MRGFLPYPIIATMFKTLIPTKFAFEMILTGKNFDAPRSMAAGLINRVVPHDQLQDEAW
                     KWAHEIGAFDKVTLKYCKMAAHASMEAASVPLAAEIAWLMQEEHALVNPRAYAGTKPF
                     HK"
     gene            795..1706
                     /locus_tag="VFY96_11825"
     CDS             795..1706
                     /locus_tag="VFY96_11825"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607199.1"
                     /translation="MVRVAYSSISGAGVVTWLAVDKGLFAKNDLDVELIYVAGSQAMQ
                     SLLSGTTPIGIQGIEPVFRVNAHGSDTVMLLSIVTKPPFSIMVRPEIKDYRELKGKAM
                     GITRYGSSTDMLLRLSLEKWGFKPETDVSILQMGGVPPILAGMESRKIVGGPLSLPTL
                     ARARKEGYRELADVADLVPEYQVAGVVTRRAFIKQNPDVVRGFVKAMAEAMVLFGKDP
                     EAVRQVMKTRFKIDDHQLIEETQKDYQRYMPKIPYPSRAGIAVIKSFLDKNEPAVRPL
                     SIDDQIDNQFVRELEQSGFIDNLYRSK"
     gene            complement(2033..>2614)
                     /locus_tag="VFY96_11830"
     CDS             complement(2033..>2614)
                     /locus_tag="VFY96_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607200.1"
                     /translation="LQEHYGVRPEECHWYTSEPERAGYQPPADVKITVVDEPTEALLL
                     RGEIDALIPPNIVPSFRAKDPRIRRVFKDARETVNEYYKKTKIFPITHALVMREALFE
                     EHPWLASSLLEAFTETEAVCRKSYAYAKRSAFPSAVLILEEEEEIFGNNPWGHGLTPE
                     NQIVLEKFVQYANEQGYIPNRPALSELFAPVGN"
BASE COUNT          596 a          674 c          769 g          575 t
ORIGIN      
        1 ctcacggttt tcgaagaact gcgcaatgat gacacgatcg cggtggtttt gaccacgggg
       61 gccggcgacg tggcctattg cgccgggcgc gatctttcgg aattcccgac cgaaggtggc
      121 aaaaaccgcg gcaaggatcg ccgcgccgaa ccgcgcgcct accatgtcgc tgaactgatt
      181 cggacctttc ccaaagtgac cgtcgcagtc gtgaacggtt tctgtctcgg cgccggcatc
      241 actttgctgc tgccgcatga tttggcgatc gcgtcggatc gggcgcagtt cggtttgcca
      301 gaaatcatgc gcggcttttt gccgtatccg atcatcgcga ccatgttcaa gactttgatc
      361 cccaccaaat tcgccttcga aatgattctt acagggaaaa attttgatgc gccgcgctcg
      421 atggcggcgg gattgatcaa ccgagtggtg ccccatgatc aattacaaga tgaagcatgg
      481 aagtgggcgc atgaaatcgg cgcattcgac aaagtcacat tgaaatactg caaaatggcg
      541 gctcatgctt caatggaagc agcgagcgtg ccgttggctg cggagatcgc ctggctcatg
      601 caggaagagc acgcgctggt taatccgcgg gcgtacgcag ggacaaagcc gtttcataaa
      661 tagagaagag cgtacaggca acaggcgagt catatatgag cacactgcga atcataaaga
      721 cttcagtgtt cgctgcagca gccttgtcaa tgatgatagt cggagcattt gttacagaga
      781 cgtcggcgag aacgatggtg cgagtcgctt acagctcgat ttcgggcgca ggcgttgtca
      841 cgtggctcgc ggtcgataaa ggtttgttcg ccaaaaacga tctcgacgtc gagttgatct
      901 atgtcgccgg ctctcaggcg atgcaatcgt tgctcagtgg cactacgccg atcggcattc
      961 aggggatcga gcctgttttt cgggtgaacg cccacggcag cgatacggtc atgctcctga
     1021 gcatcgtgac caagccgccg ttttccatca tggtgcggcc ggagatcaaa gactatcgcg
     1081 agctcaaggg caaggcgatg gggattacac gctatggttc gtcgaccgat atgctgctgc
     1141 gtctgtcctt ggagaaatgg ggattcaaac ccgagaccga cgtgtcgatt ttgcagatgg
     1201 gcggcgtgcc accgatactc gcgggcatgg agtcgcgcaa aatcgtcggc gggccactgt
     1261 cgttgccgac cttggcgcgc gcgaggaaag aaggttaccg cgaattggcc gatgtggcgg
     1321 atctggttcc cgaatatcag gtggcgggag tcgtgactcg gcgcgcgttt atcaagcaga
     1381 accccgacgt cgtgcgaggg ttcgtgaagg cgatggcaga ggcgatggtg ttattcggca
     1441 aggatcctga agcggtaaga caggtgatga aaacgcgctt taagatcgac gatcatcagc
     1501 tgatcgagga aactcagaaa gattatcagc gctatatgcc caaaattccc taccccagtc
     1561 gagcgggcat cgcggtgatc aaatcatttc tcgataagaa cgagcccgcc gtccggccgc
     1621 tgtcgatcga cgatcagatc gacaatcagt tcgtgcgcga gctggagcaa agcggtttca
     1681 tcgataatct ttaccgttca aagtgaggga aaagcgtttt tgagcaaacc gctgcggata
     1741 tttatagcgt cgttcaaacg aacttcaatg acaccggcat tccgacgcag gaaggcatcg
     1801 gcaatatcat tcgcgcgatc aaggccgagg ggcgtttcgt cgatcgcaaa atcacgttcg
     1861 aagaggtcgc cgagccccga ttggcgaccg aagtcgcgaa ggagttggga tacaaggtgc
     1921 cgtcagagag atggactggc ggagtgatgg atatcgcttc gctccaccct acggatacta
     1981 ggcagaccgt tcgctttgct cagtgtgact gattgccctc agaggcaatc agtcagtttc
     2041 ccaccggagc gaagagttca ctcaaagccg gccggttggg aatgtaccct tgctcgttgg
     2101 cgtactgcac gaatttttcc aagacaattt gattctccgg cgtcaggccg tggccccatg
     2161 gattattgcc gaaaatttcc tcctcttcct ctaaaatcag cacagcactc ggaaaggcgg
     2221 agcgcttcgc gtaagcgtag gatttccggc aaaccgcttc ggtttcagta aacgcctcga
     2281 gcagactcga agccagccag ggatgttcct cgaagagcgc ctcgcgcatg accaaagcgt
     2341 gtgtgatggg aaaaattttc gtctttttgt aatactcatt gaccgtttct ctggcatctt
     2401 taaaaacgcg ccgaatgcgc ggatctttgg cgcgaaacga tggcacaata ttgggtggaa
     2461 ttagcgcgtc gatctcaccg cgcagcagca atgcttcggt cggctcatca acgacggtga
     2521 ttttgacgtc agcgggcggt tgatagccgg cgcgctcggg ttcggaagta taccagtggc
     2581 attcttccgg acgtacccca taatgttcct gcag
//
LOCUS       DASULZ010000574         9999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_776708, whole genome shotgun sequence.
ACCESSION   DASULZ010000574 DASULZ010000000
VERSION     DASULZ010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9999
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776708"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VFY96_11835"
     CDS             <1..485
                     /locus_tag="VFY96_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX5607201.1"
                     /translation="LKLWPTHGDGKWKSKLMLKDSIADVTLQFVLIRPKDYDVIATCN
                     LNGDYLSDALAAQVGGIGIAPGANINYETGHAVFEATHGTAPKYADLDKVNPGSVILS
                     GEMMLRYMGWSEAADLIIKGIEGAVNAKTVTYDFERLMPGSKLVSCSGFGDAIVANMQ
                     "
     gene            546..1490
                     /gene="mdh"
                     /locus_tag="VFY96_11840"
     CDS             546..1490
                     /gene="mdh"
                     /locus_tag="VFY96_11840"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX5607202.1"
                     /translation="MARKKIALIGAGNIGGSMAHLALLKGLGDVVMFDVIEGLPQGKA
                     LDLSHAGPVEGFDGNVIGTNKYDDIAGADVCIVTAGIARKPGMSRDDLLGTNAKILSS
                     VADGIKKHAPDSFVIVITNPLDAMVTLMQRALGFPKNRVVGQSGILDSSRYRSFVAKE
                     LNVSVKSVQAMVLGGHGDDMVPVRSSCLVGGVPVEKLIGAKRLDEIEARVRNAGGEVV
                     ALLKTGSAYFSPASAAIQMTEAYLFDKKEILPCAALLEGEYGVTGYYFCVPVIIGAGG
                     VEKIIDLNLSAADKKAFDESLNHVKDIVQAMNRVLGVA"
     gene            1573..2742
                     /gene="sucC"
                     /locus_tag="VFY96_11845"
     CDS             1573..2742
                     /gene="sucC"
                     /locus_tag="VFY96_11845"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015863432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX5607203.1"
                     /translation="MNIHEFQAKEILKRFGVAVPRGLVATTAEEAQAAAQQLGGGVIV
                     VKAQIHAGGRGKGGGVKVVKSPQEAADKARQMLGKNLVTHQTGPEGRQVRRVLVEQGV
                     EIERELYLAMVLDRAQSRVTVICSSEGGVEIEEVAAKTPQKILKETVDPVIGLAAFQC
                     RRLAFALNIPSELIGKTVAVMQGLYRAFEETDASLAEINPLIITKDGQIMALDAKMNF
                     DSNAFFRQKEIVAMRDLDEEDPREVEASKYDLSYISLDGNIACMVNGAGLAMATMDII
                     KHYGGEPANFLDVGGGADKEKVAQAFRILLADSRVRGVLINIFGGIMRCDVLAQGVVD
                     AARELKVIVPLVVRMQGTNVEQGRQILEGSGLTIISADTMAEAAEKIVSAVRRQA"
     gene            2793..3668
                     /gene="sucD"
                     /locus_tag="VFY96_11850"
     CDS             2793..3668
                     /gene="sucD"
                     /locus_tag="VFY96_11850"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018670810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX5607204.1"
                     /translation="MSILVNKNTRVLTQGITGATGQFHTRACKEYGTQMVAGVTPGKG
                     GASFEGIPIFDTVARAVKETGANASVIYVPPPFAADAILEAVDAEIALVICITEGVPV
                     RDMVKVKRYLEGRNSLLLGPNCPGTITPGECKIGIMPGHIHKPGTIGVVSRSGTLTYE
                     AVDQLTRLGMGQSSCVGIGGDPVHGLEFVDVLKMFNDDPQTNGVVMIGEIGGSAEEEA
                     AAWIKANMKKPVAGFIAGQTAPPGKRMGHAGAIISGGKGTAREKISAMTAAGIRMASS
                     PATIGSTMQEAISVK"
     gene            3665..4084
                     /gene="ndk"
                     /locus_tag="VFY96_11855"
     CDS             3665..4084
                     /gene="ndk"
                     /locus_tag="VFY96_11855"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010962081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX5607205.1"
                     /translation="MSIERTLSIIKPDAVGRNLIGEILRRFEGAGLRIAAGKLLRLSP
                     ERAQAFYAIHKERPFFASLCAYMSSGPIFVSILEGENAIAKNREIMGATDPAKAAPGT
                     IRKDCGKDVEQNAVHGSDGPETAASEIAFFFRPDEIV"
     gene            4145..5218
                     /gene="rlmN"
                     /locus_tag="VFY96_11860"
     CDS             4145..5218
                     /gene="rlmN"
                     /locus_tag="VFY96_11860"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX5607206.1"
                     /translation="MKPNIADLSSKDLSRFLAEHNHSTYRAKQIWQWLFQKRATTFGE
                     MTNLSRELRELLMNHFVIGRLQILRRAESSDGTVKFLFGLADGESIESVLIPEATRLT
                     LCISTQAGCAFGCAFCATAVLGLKRNLTSSEIVDQILEVSRTLSDKRRITHVVLMGMG
                     EPLANYDQTLRALQIITDSQWGIGISPRRVTLSTVGLVPQIEQLMAETKVNLAISLHA
                     PNNELRGRLMPVNRKYSLAQLIDCCRSLPIPRRKRITFEYVLLHGVNDSNEQARELAK
                     LLRGIPSKVNVIPFNPHPGSGFLRPTNESIARFQNLLHEAGVQINVRRPRGDDIQAAC
                     GQLQGEEAGRERRDKRRAEATVS"
     gene            5215..6075
                     /gene="mtnP"
                     /locus_tag="VFY96_11865"
     CDS             5215..6075
                     /gene="mtnP"
                     /locus_tag="VFY96_11865"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEX5607207.1"
                     /translation="MKSAVIGVIGGSGLYQMPELAGVREVEIKTPFGKTSDPLIRGRL
                     GRVDLIFLPRHGKGHRWLPTEINFRANIFALKKLGVERIISVSAVGSLREEIAPGHLV
                     VPDQFIDRTTQRPSTFFGRGVVAHVSLANPFCSELSKVLVMAARQEGAEVHSGGTYLC
                     MEGPQFSTRAESHLYRAWGAHIIGMTNLQEAKLAREAEICFGTLALATDYDCWNDQAG
                     DVEIEHVLTVLKQNVALAQRTIARAAAELSAPRACGCVSALKDAIITERARIPKKIRT
                     ELQPIIGKYL"
     gene            6072..6977
                     /locus_tag="VFY96_11870"
     CDS             6072..6977
                     /locus_tag="VFY96_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX5607208.1"
                     /translation="MSVLVVGTVAFDSIETPFGSAERILGGSASYFALGASFFAPVRV
                     VGVIGEDFPQDYIDTFNQRQIDLEGLQRDKGDTFHWRGRYHEDINLRDTLELHLNVLA
                     GFEPKLPEGYRDADYVFLGNIDPVMQLEVLRQLRSMKLVVCDTMDHWIRESQEELKKV
                     LQRIEILVINDSEARLLSGYDNIVKAARAILKMGPKMVLIKRGEYGVLQFSDSSVFAT
                     PAYPLEEVFDPTGAGDSFAGGFMGHLARSGDQSQGGLRRAIVYGSVVASFTVEDFGVK
                     RLTDVSLAEIEERYQRFIDLTDFHS"
     gene            6928..7935
                     /locus_tag="VFY96_11875"
     CDS             6928..7935
                     /locus_tag="VFY96_11875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009782163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5607209.1"
                     /translation="MKNAINGLSISPIFIPEPMELSVVIPVYNEEENIEPLMREIHGV
                     LGPLGKTYEIITVDDGSKDGTFTALRKIRCMLPIVKAVRLKRNFGQTAALAAGLAYAD
                     GEIIVLMDGDGQNDPADIPVMINVLEEGNDLVAGWRYNRKDPFFRRRLPSMLANYLIS
                     WATSVKLHDYGCTLKAMRKPVAKNLRLYGEMHRFIPAIAYERGARIAEVKVNHRPRIR
                     GESKYGISRTLRVVLDLLTVKFLSSYSTRPAHVFGPLGVISGLIGFALAVYLTVQKLV
                     YRLEIGNRPLLLLAILLIFIGFQFVTMGLLGEMLARTYHESQDRPVYVVGEVLQSGGK
                     A"
     gene            7932..9236
                     /locus_tag="VFY96_11880"
     CDS             7932..9236
                     /locus_tag="VFY96_11880"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX5607210.1"
                     /translation="MNLAVIGTGYVGLVAGTCFAETGNDVVCVDIDEEKIAMLKSGKV
                     PIYEPGLEEMVRRNAEDQRLIFTTELASAVKKSEIVFIAVGTPQSATGHANLSYVEAT
                     ARGIGKAMNGFKIIVTKSTVPVGTADLIKGWLSEETRQPFAVASNPEFMKEGAAVEDF
                     MKPDRVVLGGDNADALETLKELYEPFLRTGNPILFMDSRSAEMSKYAANAMLATKISF
                     INEIACLCEEMRADVSEVRRAISLDRRIGPHFIFPGVGYGGSCFPKDIRAMISMAGDN
                     RAMPLLTAVEQVNELQKGRLFDKVKNHFGDDLSRLTFAIWGLAFKPRTDDMRDAPSIV
                     VIKALLEAGSRVQAFDPEAMEEARKIFGDRIQYTHRNYDALHGAAALLILTEWNEFRR
                     PDFARIKSLLKQPVIFDGRNIFDPADLRKLGFTYYSIGRNDG"
     gene            9229..>9999
                     /locus_tag="VFY96_11885"
     CDS             9229..>9999
                     /locus_tag="VFY96_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018046599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5607211.1"
                     /translation="MARYLVTGGAGFIGSHLCESFLNRGHEVLCMDNYSTGAKQNIGA
                     FLKNPRFRFIDHNVSRYIEIPEPLDYILHFASPASPVDYLELPIPTLKVGALGTHNTL
                     GLAKAKSAVYLLASTSEVYGDPLVRPQSEEYWGNVNPIGPRGVYDEAKRFAEAMTMAY
                     HRFHGLNTRIVRIFNTYGPRMRLHDGRVVPNFIMQALKGEPLTVYGRGEQTRSFQYVD
                     DLVAGLNKLIESDHHLPVNIGNPHEMTVLEFAKKIIELT"
BASE COUNT         2206 a         2611 c         3010 g         2172 t
ORIGIN      
        1 cgctcaagct ctggccgacg cacggcgacg gcaagtggaa atcaaaattg atgctcaaag
       61 actccatcgc cgacgtgacc ttgcaattcg tgttgatccg tccaaaagac tacgacgtca
      121 ttgccacctg taatctcaac ggcgattatc tctcggacgc attggccgcg caggtcggtg
      181 gaatcggtat tgcgccgggc gccaacatca attacgagac tggccacgcg gtgttcgaag
      241 ccacccacgg caccgcgccg aagtacgctg atctcgacaa ggtcaacccg ggttcggtga
      301 ttctatccgg cgagatgatg ctgcgttata tgggctggtc ggaagccgcc gatctgatca
      361 tcaagggcat cgagggagcg gtgaacgcga agacagtgac ctatgacttt gagcggctca
      421 tgccgggttc caagctggtc agctgctcgg gcttcggtga tgcgattgtt gctaatatgc
      481 aatagcgctg ctggccttca ggtgccaggt ttcaggtggt aagcttttcg aatagcggga
      541 gagttatggc taggaagaag atcgcgttga tcggcgcagg gaacatcggc gggtcgatgg
      601 cgcacctggc gctgctcaaa ggactcggcg atgtcgtcat gttcgacgtc atcgagggac
      661 tccctcaggg caaagcactc gatctctcgc atgccgggcc ggtagaagga ttcgatggca
      721 atgtcatcgg gacaaacaag tatgacgaca ttgccggtgc cgacgtttgc atcgtgaccg
      781 caggcatcgc ccgcaagccc ggcatgagcc gagatgactt gctcggaacc aacgccaaaa
      841 tactgagttc ggtggcggac gggattaaaa agcatgcgcc ggattctttc gtcatcgtga
      901 tcaccaaccc gctcgacgcg atggtgacct tgatgcagcg cgccttgggc tttccgaaaa
      961 atcgcgtcgt cggccaatcg ggaattttgg attcttcgcg ctatcggtct tttgtcgcaa
     1021 aggaactgaa cgtctcggtg aagagcgttc aagcaatggt tttgggcggc catggcgatg
     1081 atatggtgcc ggtccgcagt tcctgtttgg ttggcggcgt acctgtagag aagcttattg
     1141 gagccaagcg cctcgacgaa attgaggctc gtgtacgcaa tgcgggcggc gaagtagtgg
     1201 cgttgttaaa aactggatcg gcctattttt cgccagcctc ggcggcgatc cagatgaccg
     1261 aagcttatct ttttgacaaa aaagagatcc ttccttgtgc ggcattgctg gagggcgagt
     1321 atggcgttac cggctattac ttctgtgtgc cggtgatcat tggcgccggt ggggtcgaga
     1381 aaatcatcga tctcaatttg agtgccgccg acaagaaggc cttcgacgaa tcgctcaacc
     1441 atgtcaaaga catcgtccag gcaatgaatc gtgtgttggg cgttgcgtag cacagtagag
     1501 ttccagtcgc ctgagaccct gagactaagc ctcttctagt cttagggtct tgttttttgc
     1561 agtccgaaag cgatgaacat ccacgagttt caagcaaagg aaatcctcaa acgtttcggg
     1621 gtagccgtgc cgcgtggatt ggtagcgacc accgcggagg aggcccaagc agcagcccag
     1681 cagcttggcg gaggagtgat tgtcgtcaag gcgcaaattc acgcgggtgg ccgcggtaaa
     1741 ggtggcgggg ttaaggtggt caagagccca caggaggccg cggacaaggc gcgccagatg
     1801 ctgggcaaga atctcgtgac gcaccaaacc gggccggaag gccggcaagt ccgccgcgtt
     1861 ctggtggaac agggagtgga aatcgagcgg gaactctatc tcgcgatggt cttggaccgc
     1921 gcgcagtccc gggtgacggt gatttgctcg tccgaaggag gcgttgagat cgaggaagtc
     1981 gccgcgaaaa ctccgcaaaa aatcttgaag gaaaccgtcg atccggtgat cgggctggcg
     2041 gcgtttcaat gccgacgctt agccttcgca ttgaatattc catcggagct gatcggcaaa
     2101 accgtagcgg tcatgcaggg gctttaccga gcgtttgagg aaactgacgc ttcgctggcc
     2161 gagattaacc cgctgatcat cacgaaggat gggcagatca tggcgttgga cgccaaaatg
     2221 aatttcgaca gcaatgcttt tttccgacaa aaagaaattg tcgcgatgcg tgatcttgat
     2281 gaagaagacc cacgcgaggt cgaggctagt aagtacgatc tctcctatat ttccttagac
     2341 ggcaatatcg cctgtatggt caacggtgcc ggcctggcca tggcgaccat ggacatcatc
     2401 aagcactacg gcggcgagcc ggcaaatttt ctcgatgtgg gcggtggcgc tgacaaggaa
     2461 aaagtcgcgc aggctttcag gattttgctg gccgattctc gagtgcgcgg cgtgttgatc
     2521 aacattttcg gcggcatcat gcgctgcgac gttctcgcgc aaggcgtggt cgatgccgcg
     2581 cgcgaattga aggtcatcgt cccgctggtc gtgcgcatgc aggggacgaa tgtagaacag
     2641 ggaaggcaga tcctggaagg ctcgggatta acgatcattt ccgccgacac catggcggaa
     2701 gcggcggaga agatcgtttc tgccgtgagg aggcaggcgt aagggggaag gggtattgtt
     2761 gcgctcaccc ctgacgactt acgagttcag cgatgtcgat tctggtcaac aaaaacacgc
     2821 gcgtcctgac tcagggaatc actggcgcca cgggccagtt tcatacgcgc gcgtgcaaag
     2881 agtacggcac gcaaatggtg gcgggcgtaa cgcccggaaa aggcggcgcc tcattcgaag
     2941 gtatcccgat cttcgatacc gtcgcgcgag cggttaagga aaccggcgcc aatgccagtg
     3001 tcatctacgt gccgccccca ttcgcggccg atgcgattct ggaagccgtt gacgcggaaa
     3061 tcgctttggt catttgtatc accgaaggcg tccctgtccg cgacatggta aaagtgaagc
     3121 gctatctcga aggccggaat tcactgctgc tgggtcctaa ctgtccggga acgataacac
     3181 cgggcgaatg caaaatcggc attatgcctg gtcacatcca caaacccgga actatcggcg
     3241 tagtttcgcg cagcgggacg ctaacctatg aagcagtgga tcaactcacg cgcttgggga
     3301 tggggcagtc cagctgtgtt gggatcggtg gtgatccagt gcatggattg gaatttgtcg
     3361 acgtcttgaa aatgttcaat gacgatccac agacaaacgg ggttgtcatg attggcgaga
     3421 tcggcggcag cgccgaggaa gaagccgcgg cgtggatcaa agcgaacatg aaaaagccgg
     3481 tggcgggttt cattgcgggg caaacggcgc ccccgggaaa gagaatgggt catgcggggg
     3541 cgatcatttc cggtggaaaa ggaaccgcaa gagaaaagat ctcggccatg actgcagcgg
     3601 ggattcgcat ggcgtcatcg ccggcaacta tcggaagtac gatgcaggag gcgatatcag
     3661 tcaaatgagc atcgagcgaa ctttatcgat catcaaaccg gacgctgtgg gcagaaatct
     3721 gatcggcgaa attctacgcc gattcgaggg ggccggactc agaatcgccg ctggaaaact
     3781 tctgcggttg tctcccgaac gcgcgcaggc tttttatgcg atacataaag aaaggccctt
     3841 ttttgcgagc ttgtgcgcct acatgtcctc gggaccgatc ttcgtttcga ttctcgaggg
     3901 tgaaaacgcg atcgcaaaaa atcgtgagat tatgggggcc acggatccag cgaaagcggc
     3961 accgggaacc atacgcaagg attgtggcaa ggatgtggaa cagaacgcgg tgcatggctc
     4021 cgacgggccg gaaactgcag cgagcgagat cgccttcttt ttcaggccgg acgaaatcgt
     4081 ttgacacccg cctctccgct cctgttcgca tccgggtcct atattgtcgt tttagctaat
     4141 tgacatgaag ccgaacatcg cagacttgag ctcgaaggat ctgtcccgct ttctagccga
     4201 gcacaaccat tcgacttatc gcgccaagca gatctggcaa tggttgtttc agaagcgggc
     4261 aacgaccttc ggagagatga ctaatctgtc gcgggagctg cgcgaactat tgatgaacca
     4321 tttcgttatc ggacggttgc agatcttgcg ccgcgccgaa tcgagcgacg gcacggtaaa
     4381 attcctgttt gggctggcag atggcgagag catcgaaagt gtgttgattc cggaggcgac
     4441 ccggttaacg ctttgcattt cgacccaagc cgggtgtgcg ttcggctgcg cgttttgcgc
     4501 cacggctgtg ctcgggctga aacgaaacct cacttccagc gaaatcgtcg atcaaatcct
     4561 cgaggtgagt cgcacgctta gcgataagcg ccgcatcacg catgtcgtgc tgatgggaat
     4621 gggcgaacct ctggccaatt acgatcagac attgcgcgcg ctgcaaatta tcaccgatag
     4681 ccaatggggc atcgggattt cgccgcggcg ggtgacgctc tcgactgtcg gcttggtacc
     4741 gcaaatcgaa cagctcatgg ccgagacaaa ggtcaatctc gctatctcac tgcatgcacc
     4801 caacaacgag ctccgcggtc gattaatgcc ggtgaaccgc aagtattcgc tggcgcaact
     4861 gatcgattgc tgccgttcat taccgattcc gcggcgcaag cgaatcacct ttgaatatgt
     4921 tttattgcac ggcgttaacg attcgaacga acaggcccgc gagctggcca aactgctgcg
     4981 cggcattccc agcaaagtta acgtgattcc ttttaacccc catcctggca gcgggtttct
     5041 ccgaccgacc aacgaaagca tcgcgcgctt tcaaaatctg cttcacgagg caggcgtgca
     5101 gattaacgtg cgccgtccgc gcggcgatga tattcaagcc gcttgtggtc agttgcaggg
     5161 cgaagaagcg ggacgcgagc gacgcgacaa gcgccgcgct gaggctaccg tttcatgaaa
     5221 tctgcggtca tcggcgtgat tggcggcagc gggctttacc aaatgccgga actcgcgggt
     5281 gtgcgcgagg tggagatcaa aacgcccttt ggcaagactt ccgacccgct cattcgaggt
     5341 cggctgggcc gtgtcgattt gatattctta ccgcggcacg gcaaaggcca tcgctggcta
     5401 ccgacggaga tcaattttcg cgccaatatt tttgcgctga agaagttagg cgtcgaacgg
     5461 attatttcag tcagcgccgt cggaagcttg cgcgaagaga ttgcgccggg tcatttggtg
     5521 gtgccagatc agtttatcga tcgcaccacg caacgtccaa gcacgttctt cggccgggga
     5581 gtggtcgccc acgtgagcct cgcgaatccg ttttgtagtg agttatcgaa agtgctcgtc
     5641 atggcggcgc ggcaagaagg cgccgaggtt cattcgggtg gcacgtatct atgcatggaa
     5701 ggaccgcagt tttccacccg agcggaatcg catctttatc gggcttgggg cgcgcacata
     5761 atcggcatga cgaatctcca ggaggccaaa ctcgcccgcg aggcggaaat ttgtttcggt
     5821 accttggcgc tcgccaccga ctatgactgc tggaacgacc aagccggcga tgttgaaatc
     5881 gagcatgtgc tcacggtgct gaaacagaat gtcgctctcg cccagcgcac gattgcgcgg
     5941 gcggcggcgg aattgtcggc gccgcgcgcc tgcggctgcg tctcggcgtt gaaggatgcg
     6001 atcatcaccg agcgcgctcg gattccgaaa aaaattcgca cggaactgca gcccatcatc
     6061 gggaaatatt tatgagtgtt ctggtcgtcg gtaccgtggc attcgattcg atcgagacgc
     6121 cgttcggctc ggccgagcgt atcctcggcg gctcggcttc ttacttcgcg ctcggtgcga
     6181 gttttttcgc gccggtgcgg gtcgtcggcg tcatcggcga ggactttccc caagattaca
     6241 tcgatacttt caatcagcgg cagatcgatc tcgagggtct tcagcgcgac aaaggcgata
     6301 cctttcactg gcgcggacgc tatcatgagg acatcaacct gcgcgacacg ttggagctcc
     6361 atttgaacgt tctcgccggc ttcgaaccca aattaccgga aggctaccgc gacgccgatt
     6421 atgtatttct cggcaatatc gacccggtga tgcaactgga agtcttgcgc cagctccgca
     6481 gcatgaagct cgtcgtgtgc gacacgatgg atcactggat tcgtgaaagc caggaagagt
     6541 tgaagaaggt acttcagcgc atcgagatcc tggtgatcaa tgattcagaa gcgcggctct
     6601 tgagcggtta tgacaatatc gtcaaggcgg cacgcgcaat tctcaaaatg gggcctaaaa
     6661 tggttctgat caaacggggt gaatacggcg tgctgcaatt ctccgattcg tccgtcttcg
     6721 ccacgccggc ctatccgctg gaagaggtct tcgaccccac aggggccggg gactccttcg
     6781 ccggcggttt tatggggcat ctagcccgat ctggcgatca aagccaaggc ggcctgcgac
     6841 gggcgattgt ctacggttca gtagtggcgt cgttcaccgt cgaggatttc ggtgtcaagc
     6901 ggttgaccga cgtctcgctt gccgaaattg aagaacgcta tcaacggttt atcgatctca
     6961 ccgattttca ttcctgagcc gatggagctt tcggtcgtca tcccggtcta caacgaggag
     7021 gagaacatcg agccgctgat gcgcgagatc cacggtgttt taggtcctct gggcaaaacc
     7081 tacgagatca tcacagtcga tgacggtagc aaggatggca ctttcacggc cctgcgcaag
     7141 atccgctgta tgctgccgat cgtcaaggca gttcggctca aacgcaactt tggccagacc
     7201 gcggcgctgg cagccggttt ggcctatgcc gatggtgaaa tcatcgtatt gatggatggc
     7261 gatggtcaga acgatccggc tgacattccg gttatgatca acgtacttga ggaaggcaat
     7321 gacttggtag ccggttggcg ttataatcgc aaggatcctt tttttcgccg ccggctgcct
     7381 tcaatgctcg ctaactattt gatctcgtgg gcgaccagcg tcaagctgca tgactatggg
     7441 tgcacactca aagcgatgcg caagccagtc gcaaaaaatc tccgactgta cggtgaaatg
     7501 catcgattta ttcccgccat cgcgtacgaa cgcggcgcgc ggattgcgga ggtcaaagtc
     7561 aatcaccgcc ccaggattcg gggcgagtcg aaatatggta tcagccgcac tctgcgtgtg
     7621 gttctggatc ttttgacggt taaatttctc tcgagttact cgacgcgccc ggcgcacgtc
     7681 ttcggtccgt tgggcgtgat cagcgggctt attggatttg ccctcgcagt gtacctgacg
     7741 gtgcaaaaac tcgtctatag gctcgagatc ggcaatcgtc cgctcctatt gctcgcgatc
     7801 cttttgatct ttatcggctt tcagttcgtc acgatgggac ttttgggtga gatgctggcg
     7861 cgtacttacc acgagtcgca agaccggccg gtttatgtcg ttggcgaggt attgcaatcg
     7921 ggaggaaagg catgaatctg gcggtgatcg gaaccggata cgtggggttg gttgcgggaa
     7981 cctgtttcgc cgagaccggt aacgacgtgg tatgcgtcga tatcgacgag gaaaagatcg
     8041 ccatgctgaa gtcgggcaag gtgccgatct atgagccggg gctcgaagaa atggttcggc
     8101 gcaatgccga ggaccagcgc ttgattttta ccaccgagtt ggccagtgcg gttaaaaagt
     8161 cggaaatcgt ttttatcgcc gtcgggacgc cgcagagcgc cacgggtcac gccaatttga
     8221 gctacgtcga ggccactgct cggggcattg gcaaagcgat gaacggcttc aaaatcatcg
     8281 tcaccaaaag caccgttcca gtgggcacag cggatctgat caaaggctgg ctgagcgaag
     8341 aaacccggca gccttttgcc gtagcatcga atccggagtt catgaaggaa ggcgcggccg
     8401 tggaagattt catgaaaccg gaccgtgtcg tcttgggtgg cgacaacgcc gacgctttgg
     8461 aaacgctcaa ggaactctac gaaccgtttt tgcgtaccgg caatcccatt ctctttatgg
     8521 acagccgaag cgcggagatg agtaaatacg ctgccaacgc gatgttggcg accaagattt
     8581 cttttattaa tgagatcgct tgcctatgcg aagagatgcg cgccgacgtg agcgaagtac
     8641 ggcgcgccat cagtctggac cggcgcattg gaccgcattt tatttttcct ggcgtcggtt
     8701 acggcgggtc gtgttttccc aaagatattc gcgccatgat cagcatggct ggggataacc
     8761 gggccatgcc gctgcttacg gcggtggaac aagtgaacga gctccagaag gggcggctgt
     8821 tcgacaaagt caaaaaccat tttggcgatg acctgtcacg cttgacgttt gcgatttggg
     8881 ggcttgcttt caagccgcgc acggacgaca tgcgggacgc tccgtcgatc gtcgtgatca
     8941 aggcgttatt ggaagccgga tcgcgggtgc aggcgttcga ccctgaggcg atggaagagg
     9001 ccaggaaaat ttttggtgat cgaatccaat acacgcaccg aaattatgat gcgctgcatg
     9061 gcgccgcagc gctgctcatt ctgaccgaat ggaacgaatt tcgtcggccg gatttcgcgc
     9121 gcatcaaaag cctgctgaag cagccggtga ttttcgatgg gcgcaacatt ttcgatcccg
     9181 cggacctgcg caagctcggt tttacctact actccatagg acgcaacgat ggctagatat
     9241 ttggtgacgg gtggcgccgg tttcatcggc tcacacctgt gcgagagctt cctcaaccgc
     9301 ggtcatgagg tgctctgcat ggacaactat tccaccggcg ccaagcaaaa catcggagca
     9361 tttctcaaga acccgcgctt tcggtttatc gatcataatg tgagccggta tatcgaaata
     9421 ccggaaccgc tcgattatat tttgcatttt gcgtcaccgg cgagtccggt ggactatctt
     9481 gaattgccga ttcccacgct caaggtggga gcgttgggaa cgcataacac gctcgggctg
     9541 gcgaaggcga agagcgcggt ttatctgctg gcatcgacat cggaagtcta cggcgatcct
     9601 ctggtgcggc cgcagagcga agaatattgg ggcaacgtga atccgatcgg accgcgcggt
     9661 gtttatgacg aggcaaagcg ctttgccgaa gccatgacga tggcctatca ccgctttcac
     9721 ggattgaaca cacgcattgt gcgcatcttc aacacgtacg ggccccgcat gcgcttgcat
     9781 gacgggcgag tcgtgcctaa cttcatcatg caggcgctca aaggcgagcc actgacggtt
     9841 tacgggcgag gcgagcagac gcgcagtttt cagtatgtcg acgatctcgt cgcgggcctc
     9901 aacaagctta tcgaaagtga tcatcacctg cccgtgaata tcggcaatcc tcacgagatg
     9961 acggttttgg aattcgccaa aaaaatcatt gagcttacc
//
LOCUS       DASULZ010000575         3389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1183535, whole genome shotgun sequence.
ACCESSION   DASULZ010000575 DASULZ010000000
VERSION     DASULZ010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183535"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VFY96_11890"
     CDS             <1..531
                     /locus_tag="VFY96_11890"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5607212.1"
                     /translation="ERDVYLMKYPQDPQGEDRPFDFLQRFARQGIYHCGAVEATQLIF
                     TGVFDRFPQLQIFWAENNVGWIPYFYQQMDQTYKVNCHWAEKLLGLSKLKKLPSEYLR
                     EHAHWGVFDDPIGIQLRHIVGVDRIMWSTDFPHIVTRWPKSLELVEEQFAGVPEDERR
                     AMLAGNAVKFFHLDAM"
     gene            complement(677..1888)
                     /locus_tag="VFY96_11895"
     CDS             complement(677..1888)
                     /locus_tag="VFY96_11895"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5607213.1"
                     /translation="MRRYWHPVALSEELPADGPLPVKIMSEDLVLFRDDCGQLGLLGL
                     HCAHRRADLSYGRVENGGLRCLYHGWLYDRHGHCLEQPCEPPEKRFCEKVHQIAYPCE
                     EYAGIIFAYMGPGEPPLFPAYEPFLAPPGHVLVTKIFHECNYFQANEGNLDPSHVSYL
                     HRQGNVPRNLKRPVQGTDGKLPLALYEADKAPHIDAEETPYGIRIFSTRSVEGDRTFF
                     RVTNFILPNKATIPGPMSGDGYNLYWHVPIDDTHHWRYDIVFRRSAPMEQKDIERNKE
                     ILDELSADYRPKRNKANRYLQDREAMKAWSFSGMGRIFNVQDTAIVEGSGPIVDRTRE
                     FLGPSDRAIILARRQVLKAIRDVQAGREAPHVIRSAKENRFPDLTVISEVITNGDEWR
                     TYWNNKLASLR"
     gene            2237..2947
                     /locus_tag="VFY96_11900"
     CDS             2237..2947
                     /locus_tag="VFY96_11900"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5607214.1"
                     /translation="MASSWQSVRVDQSEMPLYASLPESGGPVPGIVVAHGQSGLENFI
                     KDTTQMFALQGYAAVAPNLYHRDGINCKDDNPTRKARLRDPSIIRDIEGAIGFLKNHP
                     RVDSAKLGIVGFCMGGRLVYLMSAATKDLKAGVMFYGSGIMVPFGEGATPFDRTREIN
                     CPIQGHFGEEDKNPSPHDMRKLDAELTKFGKEHEFHSYGGAAHAFVNTGSSNYRPHAA
                     ALSWPKAMEFFSRHLVGK"
BASE COUNT          759 a          881 c          964 g          785 t
ORIGIN      
        1 gagcgcgatg tgtatctcat gaagtatccg caggatccgc aaggcgagga ccgcccgttc
       61 gactttctgc agcgcttcgc gcggcagggg atttatcatt gcggcgcggt ggaagcgacg
      121 cagttaattt tcaccggagt gtttgaccga tttccgcagc tgcaaatatt ttgggcggaa
      181 aacaacgtcg ggtggattcc ctacttttac cagcaaatgg accagaccta taaagtcaac
      241 tgccactggg cggaaaagct tttgggattg tcgaagctga agaaactacc gagcgaatac
      301 ctgcgcgaac atgctcattg gggtgtattc gacgatccga tcggcatcca actccgccac
      361 atcgtcggcg tcgatagaat catgtggtcg acggattttc cgcacatcgt gacccgctgg
      421 ccgaagtcgc tcgaactggt cgaagagcaa ttcgccggcg taccggagga tgaaagacga
      481 gcgatgctgg ccggaaatgc cgtgaagttt tttcatctcg atgcgatgtg aggctgagat
      541 cgttcgcaag cgggaatcca ggcggaaatg cactggacgc cgattacagc attcgggggt
      601 gacgctttga ggcggtttca ctgcgttgga atgggcgagg ggaatttctt caatccataa
      661 gcaataaacg agggtgttag cgaaggctag cgagcttgtt gttccagtag gtccgccact
      721 cgtcgccgtt ggtgatcacc tcggaaatca ccgtgagatc cggaaagcga ttttctttcg
      781 cgcttcgaat cacgtgcggt gcttcgcgac cggcttgaac atcgcgaatc gcctttaaaa
      841 cctggcgccg ggcgagaatg atcgcccgat cgctgggtcc taagaattcc cgggtgcgat
      901 caacgatcgg accgctacct tcgacaatcg cagtgtcctg aacgttgaag atccgcccca
      961 tgccggaaaa gctccaagcc ttcatcgctt cacgatcctg aaggtaacga ttggctttgt
     1021 tacgtttcgg ccgataatcg gcgctgagtt cgtcgaggat ctccttattt cgctcgatat
     1081 ccttttgctc catcggcgcg ctgcggcgaa agacgatgtc atagcgccag tgatgggtgt
     1141 catcgatcgg cacgtgccaa tagaggttgt aaccatcccc ggacatggga ccgggaatcg
     1201 tcgctttatt cggcagaata aaattggtca cacgaaaaaa agtccgatcg ccttcgacac
     1261 tgcgcgtgga aaaaatgcga atcccgtaag gggtttcttc ggcatcgata tgcggcgcct
     1321 tatcggcttc atacagggcc agcgggagct tgccgtcggt cccttggacc ggccgcttca
     1381 aatttcgtgg aacattgcct tggcgatgaa gataagaaac gtgactggga tccaggtttc
     1441 cttcattggc ttgaaagtaa ttgcactcgt gaaaaatttt cgtgaccagc acgtgccctg
     1501 gtggtgcgag gaagggctca taggcgggaa aaagcggtgg ttcaccgggt cccatgtagg
     1561 cgaagatgat ccctgcgtac tcttcgcaag gatacgctat ttggtgcacc ttctcgcaga
     1621 atctcttttc gggaggttcg cagggctgtt cgagacaatg gccatgccgg tcataaagcc
     1681 atccgtggta aagacagcgc aacccaccgt tctccacccg accgtagctc aggtccgcgc
     1741 gccgatgcgc gcagtggaga cccaatagcc cgagttggcc acagtcgtcg cggaagagca
     1801 caagatcttc actcatgatc ttcacaggca gtggcccgtc agccggcaac tcttccgaga
     1861 gcgctaccgg gtgccaatag cgtctcataa aatctccgcc ggctgttccg ggaccggtca
     1921 gagtgatcag ggcgttttct tcttgagaga gcataaggtc attttagatt tgcgatcgcc
     1981 gacgggcgga gtgtcatccc actccgagac ttagacggcg gaatttaccg cgtgccttcg
     2041 acttcacact cgggacagtt tggatttcca ttgttatacg gcctctgctt cttttatcat
     2101 tgtgcccggg cggcgacaat ccaaagatga aggggcgagc gccggtcggc gtctagaaga
     2161 aatagaattg gaaacgcgct tgcgcgcggc cgcggaattc atctatagaa cacgatgaaa
     2221 attatggagg tatgaaatgg cgagcagttg gcagtctgtc agagtcgatc agagcgagat
     2281 gccgctttat gcgagtttgc cggaaagcgg cgggccagtt ccgggcatcg tggtggctca
     2341 cggccagtca gggttggaaa atttcatcaa agacactaca cagatgttcg cgctccaagg
     2401 ctatgcggcc gtggcaccga atctttatca tcgcgacggt ataaattgta aggacgacaa
     2461 tccgactcgt aaggcgcgtt tgcgtgatcc ctcgattatc agagatatcg aaggggcgat
     2521 tggctttctg aaaaaccatc cgcgcgtcga tagcgcaaag ctgggcattg tcggattctg
     2581 catgggtggg cggctggttt atctcatgtc ggccgctact aaagatctga aagcaggcgt
     2641 gatgttttat ggaagtggga tcatggtgcc tttcggtgag ggtgctacgc cgttcgaccg
     2701 cacgcgtgaa atcaattgtc cgatccaggg gcatttcggc gaggaagaca agaatccgtc
     2761 gccccatgac atgcgcaaac tcgacgccga gctcacgaaa tttggcaagg agcacgagtt
     2821 tcacagctac ggcggtgccg ctcatgcatt cgtcaacacc gggtcgtcga actataggcc
     2881 gcacgcggcg gcactctcgt ggcccaaggc gatggaattc ttcagccggc atttggtcgg
     2941 caaataagtt cgggttaatc acttaattgc tcgatccgct ttcaggagca atgtttgagg
     3001 tacttaaacc catcggtttg gctgctttca agtttatcac aactcaaatt tggtgcattg
     3061 cttgaccggt agatcagccg gcttcgcgcc tttgataatt ttgtccacga agttgccgct
     3121 cggctatccg ggtcggtaat gttcacacca taatatatga gcccaccggc ttcgacctct
     3181 tctcgaaggc gatacatcac cggaagccgg ctctttgtcg cgaggtccac aacgtacgat
     3241 cgatggctag ccgcaacggc gctcgccaac atcacaacga cggcttcagc tcactctttg
     3301 gtcgctgctc ggaatgcgct ctcaatatcg ttgtgactca ggacatccac gtattgaagc
     3361 ttcactccga aaggcctttg cggcgagct
//
LOCUS       DASULZ010000576         2484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_67961, whole genome shotgun sequence.
ACCESSION   DASULZ010000576 DASULZ010000000
VERSION     DASULZ010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67961"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..1062
                     /locus_tag="VFY96_11905"
     CDS             181..1062
                     /locus_tag="VFY96_11905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX5607215.1"
                     /translation="MKLGLQIPSFTWPGGAEQISSTLVRIGQSAEEAGFASLWVMDHF
                     FQIEMIGKPDEPMLEGYSALNFLASVTRRIRLGTLVTGVIYRYPGILIKAVTTLDVLS
                     GGRAYFGIGAGWYEHEAKALGVPFPPLKERFERLEETVQIALQMWSGKSEPYDGKHYR
                     LAQTLSVPQPIRKPHPPIMIGGAGEKRTLPLVAKYADACNLYAFENTDILRAKLDVLR
                     RHCDAIGRPFQGIERTAIGTIDLRPGHVSAREAIEYCRRVSDAGIQHFIVSLPGDYEL
                     KPIEIMRREIIPAVGEL"
     gene            1210..2400
                     /locus_tag="VFY96_11910"
     CDS             1210..2400
                     /locus_tag="VFY96_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007840228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HEX5607216.1"
                     /translation="MKRSGVADLPLHGGRVPQWLAKRMTQLGTAIAEAIVRDYGTSIF
                     LSRLSDPFWFQALGSVMGMDWHSSGITTSVMGALKRGLAPRAAELGLYVCGGRGRFSR
                     NTPQELRAIADRRGLDGEALVRSSRLTARIDNNAIADGFQIYLHSFIVTANGEWAVVQ
                     QGLNDDSGMARRYHWHSASVRDFVAAPHSAIVGENQGTIMNLVDAQAKPAQDALLEIT
                     REKPETTLKDARYLQLPARHEVRAVDIDLKRLGAVLALAYDREFHDFAELLLLEKLGP
                     RTLQSLALIAEVVHGAPSRFADPARFSFAHGGKDRHPFPVPLKTYDESLNWLRVSLDR
                     AKLGDREKLDGFRRLNQFVAAIETELEPQADLEAVIRHEQAISPSLDGRSVFGGRSKQ
                     LSLF"
BASE COUNT          520 a          731 c          673 g          560 t
ORIGIN      
        1 cacatcaaag atgtacatgg cagtctgtga aagaaatcgc ccgaaatcaa ctccgtgcgc
       61 gtgcagattc gtcggcaaaa atgaccgttg gggtctatgg tcgaacccag gaattgctag
      121 gatgagtttg aagccgccaa acgatattgc cggtacggcg gcacgcctgg aggtgagatc
      181 atgaagttgg gacttcagat accttctttt acctggcccg gcggtgcgga gcagatttct
      241 tcaacgctgg tgcgcatcgg ccaatctgct gaggaagccg gttttgccag tctgtgggtg
      301 atggatcatt tttttcaaat cgagatgatc ggcaaacccg atgagccgat gctcgaaggc
      361 tacagcgcgc tcaatttttt ggcgagcgta actcggcgaa tccgtctcgg cactctcgtt
      421 accggcgtaa tctaccgcta ccctggtatt ctgatcaaag ccgtgacgac cctcgatgtc
      481 ctctcgggcg gccgcgccta tttcggcatc ggcgccggct ggtacgaaca tgaagcaaaa
      541 gcgctcggtg ttcccttccc gccgttaaaa gagcgtttcg agcggctcga ggaaactgtt
      601 cagatcgcgc tccagatgtg gtcgggcaag agcgaacctt atgacggcaa acactatcga
      661 ttggcccaaa cactgagcgt gccgcagccg attcgcaagc ctcacccgcc aatcatgatc
      721 ggcggtgccg gcgaaaaacg cactcttcct ttggtcgcga aatacgcgga tgcatgtaat
      781 ctctacgcct tcgagaacac cgatatatta cgcgccaagc tcgatgtcct gcgccgtcat
      841 tgcgatgcca tcgggcggcc cttccaaggg atcgagcgca cagcgatcgg caccattgat
      901 ctgcgcccag gccatgtcag cgcgcgtgaa gccatcgagt actgccgtcg cgtcagtgac
      961 gccggtattc aacacttcat cgtcagtttg ccaggagact acgaacttaa gccgatcgaa
     1021 ataatgcgca gagaaattat tcccgcagtc ggagaactgt aggcatcgcc tgacgccaac
     1081 gactatgttt gacgcggact catttcctta atgaggcaat gcgaaattaa atcctctgtg
     1141 tgtccctctc tgacaaataa gaacgttacc tgccgttgcc ctttcgttag atcaagctaa
     1201 aatcacttca tgaaacggtc cggagtcgca gatcttcccc ttcacggcgg ccgggtgccg
     1261 caatggctgg cgaaacgcat gacgcagctc ggcaccgcga tcgcagaggc gatcgttcgc
     1321 gactacggca cctcaatctt tctttcccgg ctgagcgatc ctttttggtt tcaagcgttg
     1381 ggctcagtaa tgggaatgga ttggcattct tcagggatca cgacctcggt catgggcgct
     1441 ctcaaacgcg gactcgctcc gcgagcggcc gagctcgggt tgtatgtttg cggcggccgc
     1501 ggtcgatttt cacgcaacac gccgcaggaa ttgcgcgcga tcgccgaccg gcgcggcttg
     1561 gatggcgaag ctctggtgcg tagcagccgg ctcaccgctc ggatcgataa caatgccatc
     1621 gcggacggat ttcagatcta cctccacagt ttcattgtca ccgccaatgg cgaatgggcg
     1681 gtcgttcagc agggcttgaa tgatgacagc ggcatggcgc gccgctacca ctggcattca
     1741 gccagcgttc gcgactttgt tgccgcgccg cacagcgcga ttgtcgggga gaatcaaggc
     1801 acgatcatga acctcgtcga cgcgcaagcc aagcctgcgc aagacgcgct gctggaaatt
     1861 acccgcgaga aacccgagac tactttgaag gatgctcgct atttgcaatt gccggcgcgc
     1921 cacgaagtcc gcgctgtgga tatcgatctc aagcgcttag gcgctgtgct tgcgcttgcc
     1981 tacgaccgcg agtttcatga ttttgccgag cttctgcttc tagaaaagct cggtccgcgc
     2041 acgcttcaat ccctcgcgtt gatcgccgaa gtcgttcatg gcgcgccaag ccgtttcgct
     2101 gatcccgcgc gtttttcttt cgcccatggc ggcaaagacc gccatccgtt tccggtccct
     2161 ttgaagacct acgatgagtc cctcaactgg ctgcgagttt ccctcgatcg agcaaaactc
     2221 ggtgaccggg agaagctcga cggctttcgc cgtctaaatc aattcgttgc tgccatcgag
     2281 accgaactcg aaccccaggc cgatctcgag gccgtgattc gccatgaaca ggcgatttct
     2341 ccgtcccttg acggccgctc cgtctttggc ggccgctcca aacagttgtc gcttttttga
     2401 ttacgctccg cgtctccgcg aaccgaactg gatttctctc agcggctttc aaattaaatc
     2461 cccctaaatc ccttttttac aaag
//
LOCUS       DASULZ010000577         2122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_776939, whole genome shotgun sequence.
ACCESSION   DASULZ010000577 DASULZ010000000
VERSION     DASULZ010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776939"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VFY96_11915"
     CDS             complement(<1..474)
                     /locus_tag="VFY96_11915"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5607217.1"
                     /translation="MDQGKILIVEDEESLRFVLQKALEAEGYWVQCAANGGLARRLLT
                     DTHFDVSLMDIKLPDIDGLDLLKQTKELGVDTAIIVMTAQNTMRNAIGAMKNGAFDYI
                     TKPFDLEEMLVLVKRAMDSRKLSRDFRELKEEVKKRFEPGVNIIGTSASMQGVYKT"
     gene            complement(480..1589)
                     /locus_tag="VFY96_11920"
     CDS             complement(480..1589)
                     /locus_tag="VFY96_11920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5607218.1"
                     /translation="MKRGAPAKRQSPTQISWEQILANLDDGVIALDAQSKIIFFNEAS
                     EMLTEISSGTAVDQSFEKIFKRELWLVDLVKTTHAPPHKRAGAEGDLVSRWGRKLPIG
                     VTVSPLRDRQGQFIGTILLLRDIKRRKELEEDLKRADRLALMGTLAAGLAHEIRNPLG
                     GIKGAAQLLRRSADGTPSVHDFTDIMIREVDRVNQLIEQLLDLSRPSQIKLAAINIHE
                     ILEDVLLLETQALTGKELYVRKRFDPSLPPIRGDRAQLTQVFLNLVKNAFQAMEAGGV
                     LTLTTRLETDYHIREPGTAANRLIWVDLADDGHGIAEEHLAHIFSPFFTTKGNGTGLG
                     LATCDRIMREHGGTIRVESVAGKGSTFKVSLVVAD"
     gene            1761..>2122
                     /gene="rimP"
                     /locus_tag="VFY96_11925"
     CDS             1761..>2122
                     /gene="rimP"
                     /locus_tag="VFY96_11925"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX5607219.1"
                     /translation="MDATTARIWELATPLAHARGMEIIDIEFRREGGRGGRVLRLFLD
                     KEGGAILDELSQVSRELSEILDGEDIIDDAYTLEVSSPGINRPLKRPDHFARFIGKKI
                     RVRTRDMINGRRSFLGRLL"
BASE COUNT          457 a          572 c          586 g          507 t
ORIGIN      
        1 ggttttgtag accccctgca tgcttgcgct cgtgccgata atattgacgc cgggctcgaa
       61 gcgcttcttg acctcttctt taagctcacg aaagtctcgg ctgagcttgc ggctgtccat
      121 agcgcgcttg accaacacca acatttcttc caggtcgaag ggtttggtga tgtaatcgaa
      181 agcgccgttt ttcatggcgc cgatggcatt gcgcatggtg ttttgcgcgg tcatcacaat
      241 gattgccgta tcgacgccga gctctttagt ttgtttgagg agatccaaac catcgatgtc
      301 cggtaatttg atatccatca gcgaaacatc gaagtgtgtg tccgtcagaa gccgccgcgc
      361 aagccctcca ttcgctgcac attgaaccca gtagccttcg gcttcgagag ctttctgcaa
      421 tacgaaccgc agcgactctt cgtcttcaac gatgagaatt tttccttgat ccatcttgct
      481 cagtccgcca ccacaagcga aactttgaaa gtgctgcctt ttcccgcaac gctttccacg
      541 cgaattgtgc ccccatgctc gcgcatgatg cgatcgcagg tcgcgagacc gagacccgtg
      601 ccgttgcctt tggtcgtaaa gaacggtgaa aaaatatgcg ccaggtgctc ttcagcgatg
      661 ccatgtccat cgtcggcgag gtcgacccag atcaaacgat tggccgccgt gcccggttcg
      721 cgaatgtgat aatcggtttc caatcgggtc gtgagggtga gcacgccgcc cgcctccatc
      781 gcttgaaaag cgtttttcac cagattgaga aagacttgcg tcaattgcgc gcggtctccc
      841 cggattggcg gcaagctcgg atcgaagcgc ttgcggacgt agagctcttt gccggtgagg
      901 gcctgggtct cgagtaacaa aacgtcctct aagatctcgt gaatattgat ggcagcaagt
      961 ttgatttgcg aaggacggga aagatccagg agctgttcga tcaattgatt cacgcgatcg
     1021 acctcgcgaa tcataatatc ggtaaaatca tggaccgaag gggtgccgtc cgccgaacgg
     1081 cgcagcagct gtgcggcgcc cttgatgccg cccagggggt tacgaatctc atgggctaac
     1141 ccggcggcca gagttcccat caacgcgagt cggtcggcgc gtttgagatc ctcttccaat
     1201 tctttccgac gcttgatatc gcgcagcagc agaatggtgc caatgaattg gccttgccgg
     1261 tctcgcaacg gtgataccgt cacaccaatg ggcagcttgc ggccccaccg gctgaccaaa
     1321 tcaccttcgg cgcccgcccg cttatggggc ggcgcatggg tggttttgac gagatcgacc
     1381 aaccagagtt cgcgtttgaa aatcttctca aacgactgat cgacggcggt cccggaagag
     1441 atctcggtca gcatttccga tgcttcgttg aaaaagataa ttttgctctg ggcatcgagc
     1501 gcgattacgc catcgtccaa gttggccaga atctgttccc atgatatctg ggtgggagat
     1561 tgccgtttgg cgggagcgcc tcttttcatg atctaggtcg aatttaaaac cggaatcata
     1621 gcaaatagcg gctagcgcgc aatggcgcga aaagccggga cttgcattgc caatattcta
     1681 catgctatta attttcttac actttaggag tcagactcga agtgggctat gcccgctttt
     1741 tttgtttctg gagccagtgg atggatgcga ccaccgcccg tatttgggaa ctcgcgacgc
     1801 ccttggcgca cgcgcgcgga atggaaataa tcgacattga gttcaggcgc gaagggggtc
     1861 ggggcggccg cgtattgcgc ctttttctgg acaaagaggg cggcgcgatc ttggacgagc
     1921 tcagccaggt cagccgcgaa ctgagtgaaa tcttggatgg cgaggacatc atcgatgacg
     1981 cttataccct ggaagtctct tcaccgggaa ttaaccgtcc actcaagcga cccgaccact
     2041 ttgcgcgctt tatcggcaaa aagattcgcg tgcgtacacg ggatatgatc aacggcagaa
     2101 gatctttttt gggccgctta ct
//
LOCUS       DASULZ010000578         6942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_777093, whole genome shotgun sequence.
ACCESSION   DASULZ010000578 DASULZ010000000
VERSION     DASULZ010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6942
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777093"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VFY96_11930"
     rRNA            complement(<1..417)
                     /locus_tag="VFY96_11930"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(625..1824)
                     /gene="tyrS"
                     /locus_tag="VFY96_11935"
     CDS             complement(625..1824)
                     /gene="tyrS"
                     /locus_tag="VFY96_11935"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX5607220.1"
                     /translation="MDLEKQLAIIKRGVVDILPEGELIAKLKQGRPLRVKAGFDPTAP
                     DLHLGHTVLIQKMRQFQELGHEVIFLIGDFTGMIGDPTGKSETRKQLTLEEVAKNAET
                     YKQQIFKILDPEKTQVKFNHSWMEKMDAVSLIGIAAKYSVARMLEREDFKQRYQKQHS
                     ISIHEFLYPLIQGYDSVVLKADVELGGTDQRFNLLVGRELQREFGQEAQVVLTMPLLE
                     GLDGVQKMSKSLGNYIGITEAPEEMFGKLMSISDALMWRYYELLSDKGLDEIQALKRN
                     VEDGSLHPMEVKKALGVEIVRRFHGVAAANQAREYFEAKYQKKEMPVEIRQHFTTSEP
                     IWICRLIVDVLKFAKSTSEARRLIAQGAVKVDGKVLHDVNFRFHSPPNEIIEVGKNRI
                     AQAAKIS"
     gene            complement(1824..2618)
                     /locus_tag="VFY96_11940"
     CDS             complement(1824..2618)
                     /locus_tag="VFY96_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEX5607221.1"
                     /translation="MRVLILGDVVGRPARRAMRDLLPGLIEREKADLVIANAENAAGG
                     MGVDIKSAKELLSAGVHVLTSGNHIWKKKEIYSFLDEHETLIRPANFPAGAPGRGWCF
                     WRHNDLRAVIINLQGRVFMPNHVEDPFRCVDELLQNHGRLSPVIIVDMHGEATSEKTA
                     MGWYLDGRVSVVYGTHTHVPTADDRILPNGTAYITDVGMCGPLDSVIGMEREVVIQGF
                     LTQLPRQFEVAKDNVALQGIITDIDEKNGRAREIRRLRAHWQATSS"
     gene            complement(2762..4300)
                     /gene="rny"
                     /locus_tag="VFY96_11945"
     CDS             complement(2762..4300)
                     /gene="rny"
                     /locus_tag="VFY96_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX5607222.1"
                     /translation="MGLIVGIGLASAFFWVRAKKERAETEAAQKTAARILEEAKKDAS
                     AIRKEAELHAKDSVLRERAEFEKEVRDSRRELQNQERRLIGKEEAIDKRLESIDKRDG
                     ELTRRDQAIKSREKQVEEQAAQYGRQLEESRRQLEQVAGLTREEAKRSLIDQMVEEAK
                     HESAKRIRVIEEEAKEEAVRRSQKIVALAIERLAGDFVAERSVTVFPLPNDELKGKII
                     GREGRNIRALEAATGIDLIVDDTPETVVISGHNPIRREIARLSLEKLVSDGRIHPGRI
                     EEVVRKSEQELEESIKEAGQKAIFDVGVHGVHPEIIKLLGRLKYRYSYAQNVLLHSIE
                     AAFICGAMAAELGLNEKQARRAALLHDIGKAVDHEVEGSHAIIGAELARKYGESPKIV
                     NAIAAHHEDVRAETILAPLVDAADALSGARPGARREMMETYTRRLEELERIVNEFKGV
                     EKSYAVQAGREIRIMVQHDLISDDEASRMAREIARKIENEMTYPGQIKVTVIREMRAV
                     DYAK"
     gene            complement(4348..4980)
                     /locus_tag="VFY96_11950"
     CDS             complement(4348..4980)
                     /locus_tag="VFY96_11950"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX5607223.1"
                     /translation="MQRAVLEESKASLRRRLLAARKSISSDQRRLWSGLIQASALRFA
                     PYLAATDLALYSPIDSEVETQAILAHALASKKAVYYPRIDPDNSAWFFQIGAETHVRP
                     GRFGIDEPAGGLPLPVYAQDSREHTLIFVPGLGFDIGGHRLGRGGGAYDRMLTALKRQ
                     AISVGLAFELQILERLPTDEWDQKIHYVITEKRIIDCGASASRNRDLIVS"
     gene            complement(5330..5608)
                     /locus_tag="VFY96_11955"
     CDS             complement(5330..5608)
                     /locus_tag="VFY96_11955"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEX5607224.1"
                     /translation="MKKALDVEILGQKFTISSDAEESYMLKVADYVDGKMQELLRASK
                     PVAKSNVAMLAALNIADEYYRLKDAHETVLNRLDQLSKKVSTTLSHDR"
     gene            complement(5616..5864)
                     /locus_tag="VFY96_11960"
     CDS             complement(5616..5864)
                     /locus_tag="VFY96_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607225.1"
                     /translation="MVYTAMTLDNIEQLEATIDRLLASHERIRTEKEAVEKKLQQKES
                     EWHHLKGQLRQFERERVELREKLDRILGQVASLDLTES"
     gene            complement(5958..6599)
                     /locus_tag="VFY96_11965"
     CDS             complement(5958..6599)
                     /locus_tag="VFY96_11965"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX5607226.1"
                     /translation="MTIRPKKPNHSSLYQKLFQGVVFLLTLAFAALASGDIAGEIQSA
                     RAERRVTQRAIKNFTLVDQNGARFEVDKLAGKVLVVAFAYTTCVDVCPLITAALRQTQ
                     IALTPKERTEVQLLTITTDPEIDSPKVLSAYAKRYGAEFENWTFLTGDPAVLARVWKN
                     FGVGVKRRARGLIDHTPLTAIVDQSKAMRVAYIGPSPDSHAVLDDVRSLLKQH"
     gene            complement(6568..>6942)
                     /locus_tag="VFY96_11970"
     CDS             complement(6568..>6942)
                     /locus_tag="VFY96_11970"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5607227.1"
                     /translation="GRNERMDLLYAERCTNCHGVNGRGDGPLAARLPARPPDFRETVE
                     RKSNNQIRRTIADGKGTMPAFAPALRPAEITDMLQMVRFLSREGRDIAWWERFDTLVV
                     AHCNIPWETVLGYDDKAEETKP"
BASE COUNT         1468 a         2010 c         1715 g         1749 t
ORIGIN      
        1 acgcggcgtt gctgcgtcag gctttcgccc attgcgcaat attccccact gctgcctccc
       61 gtaggagtct ggaccgtgtc tcagttccag tgtggctgat catcctctca gaccagctaa
      121 ccatctttgc cttggtaggc cattacccca ccaactagct aatggtgcgc gggcccatct
      181 ccgagcgata gcttataaat agaggccacc tttgacctca actcccgaag aagtcgtggt
      241 cttatttggt attagcccgt ctttcgacag gttatcccaa tctcagaggc aggttaccca
      301 cgtgttactc acccgtgcgc cactttactc agggccgaag cccctttctc gttcgacttg
      361 catgtgttag gcacgccgcc agcgttcgtt ctgagccagg atcaaactct ccagttaaat
      421 ctttcaatcc accgttaggt ggaggattga ctgttgagtg tgatagctgt gctcaaggct
      481 ttacggaaac cctaaaccgt cactgcttat aaatggcagt tgctactatt tagttttcaa
      541 agaccagaag ggtcgctctt gatgagcgaa agatctttat aggtaatgag gtgctctatg
      601 tcaataccgg gcaagagaaa atttttagga aatttttgcc gcttgcgcga tgcgattttt
      661 ccccacctct atgatttcat ttggcgggga atgaaaccgg aaatttacgt cgtgaagcac
      721 cttgccatcg actttgaccg cgccttgtgc gatcaatctg cgcgcctcgc tcgttgattt
      781 cgcgaatttg agcacgtcta cgatcagtcg gcagatccag atcggttccg acgtggtaaa
      841 gtgctgcctg atttccacgg gcatctcttt tttttgatac ttcgcctcga agtattctcg
      901 cgcttgattc gctgccgcga cgccgtgaaa tcgtcgaaca atctcaaccc cgagagcctt
      961 tttcacttcc attgggtgga gcgaaccgtc ttccacattg cgcttgagtg cttgaatttc
     1021 gtcgaggccc ttatcgctca gcagttcgta atagcgccac atgagcgcat ccgagatcga
     1081 catcagcttg ccgaacattt cctcgggcgc ctccgttatg ccgatataat tgcctagtga
     1141 tttactcatc ttctgcacac catcgagccc ttcgagcaaa ggcatggtca gcaccacctg
     1201 cgcttcctgg ccaaattccc gctgcagttc tcgaccgacg agtagattga accgctgatc
     1261 ggttccaccg agttccacgt ccgctttcag cacaaccgaa tcgtaacctt gaatgagcgg
     1321 ataaaggaat tcgtgaatgc taatcgagtg ctgcttttga tagcgttgtt tgaagtcctc
     1381 ccgctcgagc attcgcgcca cgctatattt cgccgcgatg ccgatgagtg agacggcatc
     1441 cattttttcc atccaactat gattgaattt gacctgggtc ttttcgggat caaggatctt
     1501 aaaaatttgc tgtttgtagg tttcggcgtt cttggctact tcctcgaggg tcaactgttt
     1561 gcgagtctca gatttccctg tgggatcacc aatcatgccg gtaaaatcgc cgatcaaaaa
     1621 aatgacctcg tgtccgagct cctggaactg cctcatcttt tgaatcagca ccgtatggcc
     1681 aaggtgaaga tcaggcgcgg tgggatcgaa tccagctttc actcggagcg gtcgcccctg
     1741 tttgagcttt gcgatcagct cgccctcggg aagaatatcc acgacgccgc gtttgataat
     1801 cgcaagttgc ttttcgagat cgatcatgag ctcgtcgcct gccaatgggc ccggagccga
     1861 cgaatttccc gcgcccggcc gttcttttca tcgatgtcgg tgatgattcc ttgaagcgcc
     1921 acgttgtcct tcgccacttc gaactgccgc ggcagctgag tgaggaaacc ctgaatgaca
     1981 acttcccgct ccatgccgat gacggaatca agcgggccgc acatgccgac atcggtgatg
     2041 tacgcagtgc cgttggggag aatccggtcg tcggcggtcg gcacgtgcgt gtgggttcca
     2101 taaaccacgg atacccggcc gtcgagatac caccccatgg cggtcttttc cgaggtcgct
     2161 tcgccgtgca tatcgacgat gatcactggc gagagacggc catgattttg tagcagttcg
     2221 tcgacacagc gaaacggatc ttcgacatgg ttgggcataa aaactctgcc ctgaagattg
     2281 atgatgacgg ctcgcaagtc gttgtgccgc caaaaacacc agcctctgcc gggtgctccc
     2341 gcggggaagt tggccgggcg gatcagagtt tcatgctcgt ctaggaatga atagatctct
     2401 ttctttttcc agatatgatt gccggaagtc agcacgtgca cgccggcgga aaggagctct
     2461 ttcgcgcttt ttatatcgac gcccattccg ccggcggcat tctcagcgtt ggcaatgaca
     2521 agatcggctt tctcgcgttc gatcagcccg ggcaaaaggt cgcgcatcgc gcgccgcgcc
     2581 ggacgcccca cgacatctcc tagaatcaag acccgcatta ggaattttcc acttcgccaa
     2641 ggaactcgat gcgagaatcc acatacctgt cattcatgcc ccgaaggcga ggaatctcgt
     2701 gttatcgtat tttaagacga gacccctccg ctcgggatga cgaccgtaaa atctaaggtc
     2761 gtcatttggc gtaatcgacc gcccgcattt cgcgaatcac ggtgaccttg atctgacccg
     2821 gataggtcat ttcattttcg atcttccgcg cgatttcacg cgccatgcgc gaagcttcgt
     2881 catcggaaat cagatcgtgt tgtaccatga tgcgaatctc gcgtccggct tggactgcgt
     2941 aggatttttc taccccttta aactcgttca cgatgcgctc cagctcttcc aaacggcgcg
     3001 tataagtctc catcatctca cggcgtgcgc cggggcgtgc cccggaaagc gcatcggcgg
     3061 catccaccag cggcgccaaa atcgtttccg ctctcacatc ctcatggtgc gcagcgatcg
     3121 cattgacgat ctttggcgat tctccgtatt tgcgcgcaag ctccgcgcca atgattgcat
     3181 gcgagccttc cacctcgtgg tccaccgctt taccgatatc gtgcagcaat gcggcgcggc
     3241 gcgcctgttt ttcgttcagc cccagttcag cagccatagc tccacagatg aaagctgcct
     3301 caatggaatg cagcaaaacg ttttgcgcgt agctgtaacg atatttcagg cgtcctagca
     3361 atttgatgat ttcgggatga actccgtgga cgccgacatc gaatatcgct ttttgtccgg
     3421 cctctttgat cgattcttcg agttcctgct cggacttgcg caccacttct tcgattcgtc
     3481 cgggatgaat gcggccgtcc gagacgagct tttccaagga gagccgtgca atctcccggc
     3541 gaatcggatt atgtccggag atcaccacgg tttctggcgt atcatcgacg atcagatcga
     3601 ttccggtggc cgcttcgagc gcgcgaatgt ttcgtccctc acgcccgata attttgccct
     3661 ttaactcgtc attgggcagc ggaaataccg ttaccgagcg ttccgccaca aagtcgccgg
     3721 cgagccgttc aatcgccaag gcgacgattt tttggctcct tcgaaccgcc tcctctttgg
     3781 cctcctcttc aatcacgcga atgcgcttgg ccgactcatg ctttgcttct tcgaccattt
     3841 gatcgatcag gctgcgtttc gcttcttccc gcgtcagccc tgcgacttgc tccaactgcc
     3901 ttctcgattc ctcgagttgc cgcccgtatt gagcggcctg ctcttcaact tgcttttccc
     3961 ggctcttaat cgcttggtct cgccgcgtca attcgccgtc acgcttgtca atcgattcaa
     4021 gtcgcttatc gatcgcctcc tccttaccga tcagccggcg ttcttgattc tgcagctcgc
     4081 gacgactgtc acgcacctcc ttttcgaatt cagcgcgttc cctaagaaca ctgtctttag
     4141 cgtggagctc cgcctctttg cgaatcgcac tcgcatcctt cttcgcttct tccaaaatcc
     4201 gcgcagcggt tttttgcgct gcttcggttt cggcccgctc ctttttggca cgaacccaga
     4261 aaaatgctga agcgaggcca attcccacaa tcaatccgat tagcccaaat aaaaaacttg
     4321 actccaatta gagcaccccc tttcaaatca cgaaacaatc aaatctcggt tccgcgacgc
     4381 cgaagcgccg caatcgatga ttcgtttttc ggtgatgaca taatgaattt tttgatccca
     4441 ctcgtctgtc ggcagccgtt cgagaatttg cagctcaaaa gctaagccga cagaaatcgc
     4501 ctgccgcttc agcgccgtga gcatccgatc gtacgcgcca ccgccccggc ccagacgatg
     4561 cccaccgatg tcgaacccga gcccaggaac aaagatgagg gtatgctcgc gactgtcttg
     4621 agcgtacacc ggtagaggaa gacccccggc gggctcgtct atcccaaagc gtcccgggcg
     4681 aacatgggtt tcggcgccaa tctgaaaaaa ccaggctgaa ttatcaggat cgattcttgg
     4741 gtaataaacc gcttttttgc tcgccagcgc gtgagctaaa atcgcttggg tctcaacctc
     4801 gctgtcaatc gggctgtaca gagccaggtc agtggcagcg agataaggcg caaaacgaag
     4861 cgcactggct tggatcaaac cgctccatag ccgacgctga tccgatgaaa tcgacttacg
     4921 ggcggccagc aggcgccggc gcaatgaagc cttagactct tccagaacgg ctctctgcat
     4981 aaaatcccag gagcagccag aagagactaa agcaaacagg ctggagaaac gtgcggctag
     5041 aaatccgacc ggcgatgcgg agaagtcggc ccccgtgctg ctcgttgtcc gaaccgcacg
     5101 ggcgttggcg ctaccctctc aatttgcaat gggatagcca ttccgaattc aggataactt
     5161 gtaacgcgct gccgtattct taaagcatcg aagagtagag taatccgtcc gatcaaatta
     5221 ttggcaaacg tttcgaccca tttcctcatc tttccacgcc gcttctgaca aacttccatc
     5281 agctcaaatg cccaaatgaa aaaaataatc cgcgatatca caaaccggct cagcggtcat
     5341 gactcagtgt cgtcgaaact tttttcgaca actggtccaa tcgattcaga actgtctcgt
     5401 gcgcgtcctt tagccgatag tattcatccg cgatattaag ggccgctaac atcgccacgt
     5461 tggatttcgc gaccggtttc gaggcgcgca acagttcctg cattttgcca tcgacgtaat
     5521 ccgctacctt cagcatgtag ctctcttcag catcgctgct gatcgtgaat ttttgcccga
     5581 gaatttctac gtcgagtgct tttttcatcg ctaaatcatg attcagtgag gtcgaggctg
     5641 gccacctgac ctaggatccg atcgagcttt tccctaagct cgacccgctc gcgctcgaat
     5701 tgccggagct gccctttcag atgatgccat tcactctctt tttgttggag ctttttctca
     5761 accgcttctt tttccgtcct gattcgttca tgactggcga ggagtcggtc gatggtcgct
     5821 tccagctgct caatattgtc gagagtcatg gccgtatata ccaagaaact tcatgttcga
     5881 gtttagtttc aacataaact attgtcaagt cgagaatgct ccgcaatcgc cgttctgagc
     5941 gctacgcgtt gcatctccta atgttgcttc aacaaagaac gcacgtcgtc aagtaccgcg
     6001 tgtgaatctg gcgatggtcc gatgtacgca actcgcattg ccttgctttg gtcgactatc
     6061 gcggttagcg gagtatggtc gatcaatccc cgcgccctgc gttttacccc gacgccaaag
     6121 tttttccaaa cccgagccaa caccgctgga tcccccgtga gaaatgtcca gttctcgaat
     6181 tcggcgccgt aacgcttagc ataagcgctc aggactttcg gtgagtcgat ttccggatct
     6241 gtggtgatgg tcaggagctg aacttcagtg cgctcttttg gtgtgagagc gatttgtgtc
     6301 tgccgcaaag cagccgtgat cagcgggcaa acgtccacac aggtggtata ggcgaatgca
     6361 acgaccagca ctttgccggc tagcttatcc acttcgaagc gcgcgccgtt ttgatcgacc
     6421 agcgtaaaat ttttgatcgc tctttgcgta acgcgccgct cggcgcgagc cgattgaatt
     6481 tcaccggcaa tgtcgccgct ggctagagct gcgaaggcga gcgttaataa gaaaaccacg
     6541 ccctgaaaaa gcttctgata aagcgaacta tggtttggtt tcttcggcct tatcgtcata
     6601 gcctagcacc gtctcccagg gaatattgca gtgagcaacc accaaggtat cgaacctctc
     6661 ccaccaggcg atatcgcgtc cttcccggct caaaaaacgc accatttgca gcatatcggt
     6721 aatctcggcg ggccgcaggg cgggcgcgaa cgcaggcatc gttcctttcc catcggcaat
     6781 ggtgcgtcga atctgattgt tgctttttcg ctcgacggtt tcacggaaat caggtggacg
     6841 cgccggtaaa cgggctgcca gcgggccatc gccgcgcccg tttacgccgt ggcaattggt
     6901 acaccgctcg gcatagagta aatccatccg ctcattgcgg cc
//
LOCUS       DASULZ010000579         3695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_575252, whole genome shotgun sequence.
ACCESSION   DASULZ010000579 DASULZ010000000
VERSION     DASULZ010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575252"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /gene="glyA"
                     /locus_tag="VFY96_11975"
     CDS             complement(<1..1090)
                     /gene="glyA"
                     /locus_tag="VFY96_11975"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX5607228.1"
                     /translation="MSFLEQTDPQVYGAIQKETQRLEGNLELIASENVVSEAVLEAQG
                     CVMTNKYAEGYPGKRYYGGCEFVDEVESLAIARAKQLFGADHANVQPHSGSQANMTVY
                     LSVLKPGENYLGMNLAHGGHLSMGSPVNFSGFIYKVISYGVSEKTETIDYDELERLAK
                     EHRPKLIVAGASAYPRIIDYARFRQIADQVGALLMVDMAHIAGLVAAGLHPSPVPYAD
                     FVTTTTHKTLRGPRGGMVLCKAEHAKTVDSKVFPGMQGGPLMHVIAAKAVALKEALSP
                     EFKVYQRQIVKNAQALAKGLMNQGFRLTSEGTDNHLMLVDLRKSELTGKVAQESLDKA
                     RITVNRNAVPFDTRSPFVTSGIRIGTPAV"
     gene            complement(1104..1574)
                     /gene="rpiB"
                     /locus_tag="VFY96_11980"
     CDS             complement(1104..1574)
                     /gene="rpiB"
                     /locus_tag="VFY96_11980"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX5607229.1"
                     /translation="MIAFGCDHGGLELKDFLIGRLRSKRIDVHDHGTFDRHSVDYPDY
                     AREVARRVATGEVDRGVLICTSGIGMSITANKFPGIRAALVQDLEGARSSREHNDANI
                     LVLSGAKTEPSLALQIVEIWLSTPFRGGRHRRRLDKIAQVELELGTTLVGESKN"
     gene            complement(1740..1976)
                     /gene="acpP"
                     /locus_tag="VFY96_11985"
     CDS             complement(1740..1976)
                     /gene="acpP"
                     /locus_tag="VFY96_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX5607230.1"
                     /translation="MAASVQARVKEIVCEQLGVSADEVTPEASFIEDLGADSLDIVEL
                     VMALEEEYEMEISDEDAEKIKTVQDVISYIEAHR"
     gene            complement(2004..2747)
                     /gene="fabG"
                     /locus_tag="VFY96_11990"
     CDS             complement(2004..2747)
                     /gene="fabG"
                     /locus_tag="VFY96_11990"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEX5607231.1"
                     /translation="MMLVDQVAIVTGASRGIGRAIALSLAQAGAYVVLNYRGNQTAAD
                     ATLATIVEGGGRGELSQFDVANESEIEQAVKKIIDGHNKIDILVNNAGVTGDNLLMRI
                     SPADWDQVIGTNLKGTILCSKIISRHMIRQRSGRILNISSVAGQMGNAGQSLYAAAKA
                     GVIGFTKTIARELASRNVTANVIAPGFIETDMTAKLSDQQREKFLQLIPLGRFGTCQE
                     VGDMVVFLAGEKAGYITGQVFGINGGLYM"
     gene            complement(2744..3685)
                     /gene="fabD"
                     /locus_tag="VFY96_11995"
     CDS             complement(2744..3685)
                     /gene="fabD"
                     /locus_tag="VFY96_11995"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX5607232.1"
                     /translation="MANNLALVFPGQGSQYVGMGKALSGDFAAAKNTFAEADDALDFP
                     LSQLCFAGPEEDLKLTENTQPAILATSVAGFRVLQAETPLRPMLVAGHSLGEYSALVA
                     AGAIEFREAVRIVRERGRLMQQAVPNGEGAMAVVLGLDMDATLALCAEASGDEIVAPA
                     NFNGGGQIVIAGTKRAVKRAMVLARKRGAKRVLDLPVSAPFHCPLMKPAAEGLEALLR
                     DVPVQSLKIGVVTNVEAEVNWSADRVKALLVEQAIRPVRWEQSVRKLDELGVRQIIEV
                     GPGKVLKNLIKRIEPAMTVENFEMSEDLAKVLAVMQS"
BASE COUNT          772 a         1119 c          986 g          818 t
ORIGIN      
        1 tgaccgccgg ggttccgata cggatgccgc tggtcacaaa cggtgagcgc gtatcgaagg
       61 gcaccgcatt gcgattgacc gtgatgcgcg ccttatcgag actctcttga gcgacctttc
      121 ctgtcagctc cgatttcctc aaatccacca gcatcaagtg attgtcggtg ccctcggagg
      181 tgagccggaa cccttggttc attaaaccct ttgccaatgc ctgggcgttt ttaacgatct
      241 gccgctggta gaccttgaat tcgggactca atgcttcctt caatgccacg gctttggcgg
      301 caatcacgtg catcagcgga ccaccctgca tgcccggaaa tactttgctg tcgacggtct
      361 tggcatgctc ggctttgcaa agcaccatgc caccgcgcgg accgcgtagc gtcttgtgcg
      421 tcgtcgtcgt gacgaagtcg gcatagggca ctggactcgg gtgcaatcct gcggcaacga
      481 gcccggcgat gtgagccatg tcaaccatca agagcgcgcc gacttgatcg gcgatctgtc
      541 gaaaacgggc gtaatcgatg atgcgggggt aagcgctggc gccggcaaca atcaatttcg
      601 gacgatgctc ctttgctagg cgctccagtt catcgtaatc gatggtttcg gttttctccg
      661 agacgccata cgaaatgacc ttgtagataa atccagaaaa attgaccggg ctgcccattg
      721 agagatggcc gccatgtgcg aggttcatac cgagataatt ctcgccgggc ttcaaaaccg
      781 aaagatagac ggtcatattc gcctgggagc cggaatgcgg ttgcacgttg gcgtggtcgg
      841 cgccgaataa ctgcttggct cgcgcgatcg ctagggattc cacctcgtcc acgaactcgc
      901 agccgccgta atagcgcttg cccggatacc cttccgcgta cttattggtc attacgcagc
      961 cctgagcctc caacaccgcc tcactgacga cgttctcaga ggcgatcaat tccaagttgc
     1021 cttccaggcg ctgggtctct ttttggatcg cgccatagac ttgcggatcg gtttgttcga
     1081 gaaatgacat aagcaccttc gcgctaattt ttagattcgc cgacaagcgt agtgccgagt
     1141 tcgagctcga cttgggcgat tttatcgagc cgccggcgat gacgtccgcc acgaaacgga
     1201 gtcgagagcc agatttcgac gatttgcaga gccagcgacg gctcggtttt ggcgccactc
     1261 agtaccagga tattggcgtc gttgtgctcg cggctggagc gcgcgccctc gagatcctgc
     1321 accagcgcag cgcggatgcc gggaaatttg tttgcggtga tcgacatgcc aattccactg
     1381 gtgcaaatga gcacgcctcg atccacctcc cctgtagcca cccggcgcgc cacttctcgc
     1441 gcgtaatcgg gataatccac cgaatgccgg tcgaaggttc catggtcgtg cacgtcgata
     1501 cgcttggagc gcaaacgtcc aataagaaaa tccttgagct cgaggccgcc atgatcgcaa
     1561 ccgaaggcaa tcataattcc atgatcctgc gccgttccct cctgagttcc cggtccgcgc
     1621 ataaagaaaa tcgcaggacg tggaaaacgc gtccacgacc ctgcgattta ggtggataac
     1681 tgaagcgatt gctcggccga tcagactgca gcgatgcgcc gcggggaagc cgaaaactgc
     1741 tagcgatgcg cctcaatgta gctaatcacg tcctgaacgg ttttgatctt ttccgcatcc
     1801 tcatccgaga tttccatttc gtactcttcc tcgagcgcca tcaccagttc gacgatgtcg
     1861 agtgagtccg ctcccaaatc ttcaatgaag gacgcctccg gagtgacctc atcggcgctg
     1921 accccgagtt gctcgcagac gatctctttg accctagcct gaacggatgc cgccatcttt
     1981 cacatacctc ctgcttcaga atcctacatg tataatccgc cgttaatgcc aaacacctgg
     2041 ccagtaatgt agccggcttt ttcccccgcg aggaatacaa ccatgtcgcc gacttcctgg
     2101 caagtgccaa aacgcccgag gggaatgagc tgaagaaatt tctcccgttg ctgatccgac
     2161 agcttcgccg tcatatcagt ctcgataaat ccgggagcaa tcacattggc cgtaacattt
     2221 ctcgaagcta gttcccgcgc aatcgttttg gtaaaaccga taactcccgc cttggcagcg
     2281 gcgtaaagcg attggccagc gttgcccatc tggcccgcca ccgacgagat gttaagaatc
     2341 ctgccagagc gttggcggat catgtgacgg gagataattt tgctgcagag aatcgttccc
     2401 ttcaggttgg tgccgataac ttggtcccag tccgcaggtg agattcgcat caacaaatta
     2461 tcgccggtga ctccggcatt gttgacgaga atatcaattt tgttatgacc gtcaatgatt
     2521 ttcttgaccg cctgttcgat ttcgctttcg ttggcgacat cgaattgaga aagctccccg
     2581 cggccacccc cctcaacgat cgttgcgaga gtggcatcgg ccgccgtttg gttgccgcga
     2641 tagttcagca cgacgtaagc gccggcctgc gccaacgaca gcgcgatggc gcgtccgata
     2701 ccgcggctgg cgccggtgac aatggcgact tgatctacca gcatcatgat tgcatcaccg
     2761 ccagcacttt agcgagatct tcagacattt cgaaattttc cactgtcatg gccggctcga
     2821 tacgcttgat caggttcttg agcacctttc ctgggccgac ttcgattatc tgccgaactc
     2881 ccagttcatc gagcttcctg accgattgct cccaacgtac ggggcgaatc gcctgctcga
     2941 ccaacaatgc tttgacgcga tccgcgctcc aattgacctc ggcttccaca ttggtgacga
     3001 cgccgatctt cagtgactgc acgggaacat cgcgcaagag cgcttccaga ccttcagccg
     3061 cgggcttcat cagcgggcaa tgaaacgggg cgctgaccgg cagatccagc actcgctttg
     3121 caccgcgttt cctggccaag accatggccc gtttaactgc gcgcttggtg ccggcgatga
     3181 caatctggcc accgccatta aagttggccg gcgcgacgat ctcatcgccg cttgcttcgg
     3241 cgcaaagcgc taacgtcgca tccatatcca accccaacac caccgccatt gcgccttccc
     3301 cattgggtac ggcttgctgc attaaacggc cgcgctctcg gacgatcctc acagcttcgc
     3361 gaaactcgat cgcgccggcg gccaccaacg cgctgtactc gccgaggctg tgtccggcta
     3421 ccagcatcgg tcgcaacggc gtctcagctt gcaaaacgcg gaagccggcc accgaagtcg
     3481 cgagaatcgc aggctgcgta ttctcggtga gttttaaatc ttcttcggga ccggcaaaac
     3541 agagttggct gagcggaaag tcaagcgcgt cgtccgcttc ggcaaaagtg tttttggcgg
     3601 cggcgaaatc gccgctcagc gccttgccca tgccgacata ctgggatccc tggcccggaa
     3661 agaccaaggc aaggttattc gccaccgcgc ctccg
//
LOCUS       DASULZ010000580         2314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1285308, whole genome shotgun sequence.
ACCESSION   DASULZ010000580 DASULZ010000000
VERSION     DASULZ010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285308"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VFY96_12000"
     CDS             complement(<1..387)
                     /locus_tag="VFY96_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5607233.1"
                     /translation="MKTQPFVVTPKDYDPALNVLGIKVTVLASNQATQGYEITRQQGQ
                     KEMGPPPHSHNWDESFYVVAGEIEFTCSGNTVVCSPGTLVHVPAGTVHSFRYLVDGCD
                     VLEITGQGMATQMFTAVSKEIPPRPPQ"
     gene            complement(882..2312)
                     /locus_tag="VFY96_12005"
     CDS             complement(882..2312)
                     /locus_tag="VFY96_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5607234.1"
                     /translation="MDATSNGEFVPVPLSPNNRAANRLAHDAATENAKRLGVSRRRFL
                     ISACGAVTALLAFNRANAAAGKRGGFFEIEPEAALEPELAQARVGGKAEFIFDVQGHF
                     VDPTGAWVKSAPADAFKWSPKTNCDLAGKPGARSYLNCLGPEEFVKDVFLDSDTDMMV
                     LSFVPSRRDAEPLTIQAADAVRRIVDRMEGTHRLLLHGRVNPNQPGDLESMDELKERW
                     NISAWKTYTQWGMDGKGYFLSDDVGTRFIEKARRLGIKVICVHKGLPFGRESYEHSQC
                     SDVGVVAKRFPDVKFLVYHSGFVAGVPERAYSGRGASDGIDTLIRSLVENGIKPNSNV
                     YAELGSTWRFLMREPTSAAHALGKLIKYCGEDNVLWGTDSIWYGSPQDQIQAFRTFQI
                     APELRAMYGYSEITPSLRAKIFGLNAARVYGIKEHEVKKHTRADRIAQERFAYNEHPE
                     PHFLTYGPKTRREFLNLLRWNGGSQA"
BASE COUNT          457 a          673 c          664 g          520 t
ORIGIN      
        1 ttgggggggg cggggtggta tctccttgct gaccgctgtg aacatctgtg tggccatgcc
       61 ctgaccggta atttcgagga cgtcgcagcc gtcgacaagg tagcgaaagc tatgcaccgt
      121 gccggcgggc acgtgcacaa gagtaccggg cgaacacacc acggtattgc cagaacaggt
      181 gaattcgatt tcaccggcca cgacgtagaa ggactcgtcc cagttgtggc tatgcggcgg
      241 cggacccatt tccttttggc cctgctgccg cgtgatttcg tagccttgtg tcgcttggtt
      301 tgaagccagc accgtcacct tgataccgag tacgttcaac gcgggatcgt aatccttggg
      361 ggttacaacg aacggttgag tcttcattat cgcctctctc aggaaagtgt tttgatcgat
      421 tgccgagatc aatccactaa atatcctgat ccggaatatt gaacaagttg tcgccgcgct
      481 tcgagaggcg gtgaattcgc gcgaaaaaga gagcgagtcc tggctcaaaa actccgatgc
      541 cccattccta gtctgggctt tgactgcccc tcgctacagt ttgcttcgca gtcttactcg
      601 ggaccggtcg cgtctcagag tgaaccaccg cgcgtgagta atgccgttcg acatgttcag
      661 gaattcctga tttgaatcat cgggacatgg atgagacact tgcagagtct atgcgatcgc
      721 atggcatcac gcgtgacgac ccgtgcagtt gccggtactg gattgccgtg gtcccggaca
      781 ggagcatgat tatgaaaccg atgcttgctc ggttgggaac ccttttatcg gcgattctct
      841 tgctcgctcc gtgcgcgcaa gccgcagaca aaagcgtttt cctaagcctg ggatccgccg
      901 ttccagcgta gcaagttcaa aaattcccgt ctcgttttcg ggccgtaggt gagaaagtgc
      961 ggttccggat gttcgttgta tgcaaagcgt tcctgggcga tgcgatcggc ccgggtgtgc
     1021 tttttaactt catgctcctt tatcccgtaa acacgcgccg cgttcagtcc gaagattttg
     1081 gcgcggagcg acggcgtgat ttctgaataa ccatacatgg cgcgcaattc aggggcgatc
     1141 tgaaaggtcc gaaacgcctg gatctgatcc tgcggtgagc cgtaccagat cgagtcggtg
     1201 ccccagagca cgttgtcttc accgcaatac ttgatcaatt tgccgagcgc atgggcagcg
     1261 ctcgtcggct cgcgcatgag aaaacgccag gtgctgccga gctccgcgta aacgttgctg
     1321 ttcggcttga tcccgttttc gaccagcgac cggatgagcg tgtcgattcc gtcgcttgcg
     1381 ccgcgtccgc tgtaggcgcg ctccggaaca ccggcaacga aaccggagtg atagacgaga
     1441 aacttcacat ccgggaaacg cttcgcgacc acgccaacgt cgctacactg actgtgctcg
     1501 taggattcgc gtccgaacgg cagccctttg tgcacgcaga tgacttttat gccgaggcgg
     1561 cgtgctttct cgatgaatcg tgtgccgaca tcgtcggaca gaaagtagcc tttgccgtcc
     1621 atgccccatt gggtgtaggt cttccatgcg ctgatgttcc agcgctcttt cagttcgtcc
     1681 atcgattcca gatcgccggg ctggttcggg ttgacgcgac catgcagcag cagccgatgc
     1741 gttccttcca tgcggtcgac gatccgccgc acggcatctg ccgcctggat cgtcagcggt
     1801 tcggcatcgc gccgcgacgg cacgaacgag agcaccatca tatccgtgtc gctgtcgagg
     1861 aacacgtctt tgacaaattc ttctggcccg aggcaattga gataactccg cgcgccgggc
     1921 ttgccggcca aatcgcaatt tgttttgggc gaccacttga aggcatcggc cggcgctgat
     1981 ttcacccatg caccggtcgg atcgacgaag tggccttgaa cgtcgaagat aaactcagcc
     2041 ttgccgccga cacgcgcctg cgcaagctct ggttcgagcg ccgcctcggg ttcgatttcg
     2101 aaaaaaccgc cgcgcttgcc cgccgcagca ttcgctctgt tgaacgccag caacgcagtc
     2161 acagcgccgc acgcagagat cagaaatcgg cggcgactta cacccaaacg cttggcgttc
     2221 tccgtcgccg cgtcatgcgc taagcgatta gccgcgcggt tgtttggcga caacggcacc
     2281 ggcacgaact caccgttgga agtcgcatcc agtt
//
LOCUS       DASULZ010000581         2992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1285344, whole genome shotgun sequence.
ACCESSION   DASULZ010000581 DASULZ010000000
VERSION     DASULZ010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285344"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VFY96_12010"
     CDS             <1..1084
                     /locus_tag="VFY96_12010"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5607235.1"
                     /translation="AYTNRCTARFSKHGGAAIMKILMFSVGIFLFVNFRADAQMPYFQ
                     GKTIRIVTGYPAGDVNDLWPRLIAQHMVKYIPGNPNFVVQNMTGASSMIAANYVYSVA
                     KPDALTLGWIAPTLYFDQLVGRKEVQYDWGKYSFIGSPSESEHHLYMRTDSPYKTIED
                     IRKASEPPKCGSGGASGTGFFFPRLLEETVGAKFTIVLGYQGGGPIDLAVEKGEIHCR
                     AMTIESYFAREPFHTWRKNNFVRSIAQSARKRDARLPETPTIYELMEQYKTPDQSRRV
                     ATVILAGGVFGRPMVGPAGIAPDRLKTLRAAFASALKDPELKAEAEKRGYEVEPVPGE
                     EMEKLAREVMAQPPAVLERMKKVLGN"
     gene            complement(1318..2145)
                     /locus_tag="VFY96_12015"
     CDS             complement(1318..2145)
                     /locus_tag="VFY96_12015"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5607236.1"
                     /translation="MGGDITANGDIHHLDHFVVAVMDPVRAEKFYSEVMGAHTLKTQN
                     SPNMTRIFMKLGENHVGLFSQGIATLPKRESASSYPRHSFQVPEAEYEAMAGKVRRHC
                     SFVREIQTEPDLGCPWKNGLVFQDSEGNFFEITKATGIAKARLHHLHFDTTDLDSSVE
                     FYKKFLNCAVEEKKNGFVVMTVPSGQCIVLNRASELSEVTKTAYRGRHFAFNVSDKNF
                     HAIVDKLHAAGIEERDDHGEREGRRPEQLGTYFREPSGFRLQITNEDSAEFARHAND"
     gene            complement(2174..>2992)
                     /locus_tag="VFY96_12020"
     CDS             complement(2174..>2992)
                     /locus_tag="VFY96_12020"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5607237.1"
                     /translation="YAIWPHLTVAENIGLVLTHGRLRLSKKQAAERIRHSLSLVQLQD
                     FETRPARLLSGGQQQRVALARALAINPALVLMDEPLSNLDARLREEVRANIKKLAVQI
                     GMTVLYVTHDQIEAMVLADRIAVMAHGEILQVGKPAELYRHPASALVAEFFGSINWIC
                     GMTHGNNRVETELGPLSVADSSHCHGAVIVGVRPEDVHLQRSSNENENRIEGKVRSSL
                     FLGDQIVSEVNVRGKILIAKGSPNEGQPSDTVSVYLPRERLVVFSDRVPGNSVG"
BASE COUNT          651 a          859 c          784 g          698 t
ORIGIN      
        1 ggcttatact aatcgatgca cagcgcgctt cagcaaacac ggaggagcag cgatcatgaa
       61 gattctcatg ttctcggttg ggattttttt gttcgtgaat ttccgcgccg acgcacagat
      121 gccgtatttc cagggcaaaa caattcgcat cgtgaccggc taccccgcag gtgacgtgaa
      181 cgatctttgg ccccgtctca tcgctcaaca catggtcaaa tatattccgg gcaatccgaa
      241 ttttgtcgtt cagaatatga ccggcgcaag ctcgatgatc gccgcgaatt acgtgtacag
      301 tgttgccaag cccgatgcgc tgaccctcgg ttggattgct ccgacgctct attttgatca
      361 gctagttggc cgcaaagagg ttcaatacga ttggggcaaa tacagcttca ttggctcgcc
      421 atcggaaagc gaacaccatc tttatatgcg cacggacagt ccttacaaaa ccattgaaga
      481 tatccgcaaa gccagcgagc cgcccaagtg cggttccggc ggcgccagcg gcaccggctt
      541 cttctttcct cgcttactcg aagaaaccgt cggcgcaaag tttaccatcg tgctcggcta
      601 tcagggcggc ggccctatcg atctcgcggt ggagaaaggc gagatccatt gccgcgcgat
      661 gacgatcgaa tcgtactttg cccgggagcc gttccatacc tggcgtaaaa ataattttgt
      721 caggagtatc gcgcaatcgg cgcgcaaacg cgatgcgcgc ttgccggaaa cgccgacaat
      781 ttacgaattg atggaacagt acaaaactcc cgatcagtcg cgccgtgttg cgacagtcat
      841 tctcgccggt ggcgtgttcg gccgccccat ggtaggacca gcgggcatcg cgcccgatcg
      901 gctgaaaacg cttcgagccg cgtttgcgag cgcgctcaaa gatcccgagc tgaaagctga
      961 agcggagaaa cggggctatg aggttgaacc ggtgccaggc gaagaaatgg aaaagctcgc
     1021 cagggaagtg atggctcaac cgcccgctgt gctcgaacgg atgaagaaag tcttgggaaa
     1081 ttaattttgc aggcggcgag ttcgccaagc gcgcccacgg actaatgtcg taacgattga
     1141 cgacgttgtg gcggttgtgg tgcccatact tactgaaata gccctcaccc tcgccctctc
     1201 ccatcggaat gggcgaggga attcccgagg aattctttct actcgccggt tccagcaaat
     1261 ctttcttcga caccctcgcc cgcttgcggg agagggaggg gtgagggtct tcaaacctca
     1321 gtcgttcgcg tgccgcgcaa attccgccga gtcttcgttc gtgatctgta accgaaaccc
     1381 gctgggttcc ctgaagtaag tgcccaattg ttccgggcgg cggccttcgc gttcgccgtg
     1441 gtcatcgcgc tcttcgatgc cagccgcatg aagtttatcg acaatcgcgt gaaaattttt
     1501 atcgcttacg ttgaaggcaa aatggcgtcc gcgatacgcg gtttttgtca cttcgctgag
     1561 ctcgctcgcg cggttcagaa cgatacattg ccctgacggc acagtcataa cgacaaagcc
     1621 gttcttcttt tcctctaccg cgcaattaag gaattttttg tagaactcga cactactatc
     1681 cagatccgtt gtatcgaagt gcagatgatg gagtctggct ttagcgatgc ctgttgcctt
     1741 ggtgatttcg aaaaaattgc cttccgaatc ctgaaacacc aggccgttct tccaaggaca
     1801 acctaaatct ggctcagttt gtatttcgcg cacgaatgag cagtgacgcc gcactttgcc
     1861 cgccatcgcc tcgtactcgg cttcaggcac ttgaaacgag tggcgcggat aagagctcgc
     1921 gctctcgcgc ttcggcaacg tcgctattcc ttgcgagaaa agccccacat ggttttcgcc
     1981 cagcttcata aagatgcgcg tcatattggg cgagttctgg gttttcaaag tgtgcgcgcc
     2041 catcacctcg ctgtaaaatt tctccgctcg cacgggatcc attaccgcaa ccacgaagtg
     2101 gtcgagatga tgaatatcgc cattcgccgt gatatcgccg cccataaaaa tctcctttcc
     2161 ttctggattt gtgtcaccct accgagttcc caggcacccg gtcggaaaag acgaccagcc
     2221 gttctctcgg cagatacacc gaaacagtgt cacttggttg cccttcgttg ggcgatccct
     2281 tcgcgatcag tatctttccc cgaacattga cttcggaaac gatctgatcg ccgaggaata
     2341 gcgagcttct cacttttccc tctatccgat tttcattttc attggaagat cgctgcaaat
     2401 gcacatcctc gggtcttacc cccacaatca ccgcgccatg acaatggctc gagtccgcaa
     2461 ccgacaaggg gccgagttcc gtctcaaccc tgttgttccc gtgtgtcatg ccacagatcc
     2521 agttgatcga gccgaaaaat tcagccacta gcgcgctggc cgggtgacga tatagttccg
     2581 cgggcttgcc cacctgcaat atttcgccat gagccatcac ggcgatccgg tcggcgagca
     2641 ccattgcctc gatttgatcg tgggtgacgt acagcacggt catgccgatt tgcaccgcta
     2701 gttttttgat gtttgcgcgg acttcttcgc gcaaacgagc gtcgagattg ctcagcggct
     2761 cgtccatcag caccaaggcc ggattgatcg cgagcgcacg cgctaacgcg acccgctgct
     2821 gttgaccgcc gctcaaaaga cgcgccgggc gggtttcaaa atcctgcaac tgcaccagcg
     2881 agaggctgtg acggattcgt tccgctgctt gttttttgga caagcgcaat cggccatgcg
     2941 tgagcactaa gccgatattt tccgcgaccg tcaaatgcgg ccagatcgca ta
//
LOCUS       DASULZ010000582         2316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1285371, whole genome shotgun sequence.
ACCESSION   DASULZ010000582 DASULZ010000000
VERSION     DASULZ010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285371"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1718
                     /locus_tag="VFY96_12025"
     CDS             <1..1718
                     /locus_tag="VFY96_12025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5607238.1"
                     /translation="LGVLTLLGALLPATQAWVGKLIVDGVVSSIKAGNDAEQVSRVFF
                     YLALELMLFLMGTALNHSRRLIQQLIQLQIANRIRSEIIRKALTLDLAFFEHPDFYDR
                     LQNARREGGYKPVELINDTFQIVQNVITMVSFAVLMLRFSPWLVLILLATSIPSFIAE
                     TRFSEQGFRLLTRRAPETRQINYLARLLTEDTAAKEIKLFNLGDTLLGRYMTLFDKFF
                     QEDKSLAVRRAAVGFALGLVATLGFYGSYAWIVWRTVQATISLGDMTLYLAIFRQGQT
                     TFQSILAGIGNIYENNLFMVHYIEFLDLKPRLEGGAARRKLPASLSQGVEFRGVGFRY
                     PDNDDWVLRGINLTMRPGEKIALVGHNGAGKTTLIKLLARLYDPTEGVILFDGIDIRE
                     VDPLDLRQKIGVIFQDFVRYHLAARENIGFGQIQAADQMDKVIGSARKSGAEAIIEKL
                     PEGYETTLGRWFHGGHELSLGQWQRIALARAFMRDAEILVLDEPTASLDAQTEYEIFQ
                     HFKELTEGKMAILISHRFSTVRMADRIIVIDSGRIAEMGSHEELLRIEGIYARLFSMQ
                     AEGYR"
     gene            complement(1839..>2316)
                     /locus_tag="VFY96_12030"
     CDS             complement(1839..>2316)
                     /locus_tag="VFY96_12030"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HEX5607239.1"
                     /translation="VRLPDELFFEGEGDLLACGDVWFAGYHIRSDVRAHQRVAEIINA
                     EVLSLELTNRWFYHLDTCFCPLGAGCALYYPAAFDRYGQEVLQSRIPELIPVCAAEAQ
                     RFACNAIIADNAIVLNEGCPEVRQSLQQLGFTVHETPLDEFIKAGGSAKCMVLTIT"
BASE COUNT          519 a          628 c          653 g          516 t
ORIGIN      
        1 ggcttggcgt gctaacgctg ctcggagcgc tgctcccggc cacgcaggca tgggtcggga
       61 agctgattgt cgacggcgtc gtaagctcga taaaagccgg caacgatgca gagcaggtca
      121 gcagagtatt tttctacctg gcactggaac tgatgctgtt tcttatgggt acagctctca
      181 accactcccg tcgcttgatc caacaactga tccagctgca aattgccaat cggattcgga
      241 gcgaaatcat ccgtaaagcg ctgaccttgg atttggcttt tttcgagcat ccggattttt
      301 acgatcgctt gcaaaatgct cggcgggaag gcggctacaa gccggtggag ttgatcaacg
      361 atacgtttca aatcgtccag aacgtgatta cgatggtttc tttcgcggtg ctcatgctgc
      421 gcttcagtcc ctggcttgtg ctgatactct tggcaacgag cattccttca tttattgccg
      481 agacgcgctt ctccgagcaa ggatttcgtt tgctgacgcg ccgcgcgcct gagacgcggc
      541 agatcaatta cttggcacgg ctgctgaccg aggatactgc cgccaaggag atcaagctct
      601 tcaatctcgg cgacactttg ctcggccgct acatgacttt atttgataaa ttcttccagg
      661 aggacaagtc gctcgcggta cgccgggccg cggtcggctt tgccttaggc ctagtggcca
      721 cccttgggtt ctacggttcc tacgcctgga tcgtttggcg caccgtgcag gcaacgatct
      781 cgctcggcga catgactttg tatctggcga tttttcgcca ggggcaaacg acctttcaat
      841 cgattctcgc cggcatcggc aatatttatg aaaacaatct cttcatggtg cactacatcg
      901 agtttctcga tctgaaaccg cggctcgagg gtggcgcggc gcgccggaaa ttgccggcat
      961 cgctttccca gggagtcgag ttccgcggtg tcgggtttcg ctatcccgat aacgacgatt
     1021 gggtactgcg cggaatcaat ttgacgatgc ggcccgggga aaaaatcgcg cttgtcggcc
     1081 acaacggtgc gggaaaaacc acactgatca agctcctggc ccggctgtat gatcccactg
     1141 aaggcgtcat tcttttcgac ggcatcgata ttcgagaagt cgacccgctc gatctgcgcc
     1201 aaaaaatcgg cgtcattttc caagattttg tgcgttatca tctggcggcg agagaaaata
     1261 tcggctttgg ccagatccaa gcggcggacc agatggacaa agtgatcgga tctgcgcgta
     1321 agagcggcgc cgaggcgatc attgaaaaac tcccagaggg ctatgagaca acgctcggtc
     1381 gctggtttca cggaggacat gagctgtcgc tcggtcagtg gcagagaatc gcgctggcac
     1441 gcgccttcat gcgcgatgcg gagattttgg tactcgacga gccgaccgca tccttggatg
     1501 cgcaaacgga atacgagatt tttcaacact tcaaagagtt gacggaaggc aagatggcga
     1561 tcctgatctc ccatcgattt tccacggtcc gcatggcaga ccggatcatt gtgatcgact
     1621 cgggacgcat agcggaaatg ggcagccatg aggagcttct ccggatcgag ggaatctacg
     1681 ctcgattgtt cagcatgcag gccgaagggt atcgatagtg tgcaacatcc atgcgcttga
     1741 aagacaacct cgcgaccaac actgcctgaa gacgacaatg taggcacagc acggtaagag
     1801 accggtcctt tctcgctgtg gcccgggatg tcagagcatc acgtgatcgt gagtaccatg
     1861 catttcgcgc tgccgccggc cttgatgaac tcatcgagcg gggtttcgtg tacggtaaat
     1921 cccagttgtt gcaaactttg ccgcacctcg ggacagccct cattcagcac gatcgcattg
     1981 tcggcgatga ttgcattgca ggcaaagcgc tgtgcctctg cggcgcaaac gggaatcagc
     2041 tccgggatgc ggctctgcaa gacttcctgt ccatagcgat caaatgcagc cggatagtag
     2101 agtgcacatc ccgcgcccag gggacaaaag caagtgtcga ggtgataaaa ccagcggttg
     2161 gtcaactcca gggacagcac ttcagcattg atgatttcag caacccgctg gtgcgcgcgg
     2221 acgtcagagc gaatgtgata accggcaaac caaacatcgc cgcatgccag taagtctcct
     2281 tcgccctcga aaaaaagctc atccggaaga cgaacg
//
LOCUS       DASULZ010000583         6627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_575809, whole genome shotgun sequence.
ACCESSION   DASULZ010000583 DASULZ010000000
VERSION     DASULZ010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6627
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575809"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1920
                     /locus_tag="VFY96_12035"
     CDS             <1..1920
                     /locus_tag="VFY96_12035"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEX5607240.1"
                     /translation="QDFQLHYVHIDDTPALMRRLAAVSVTSREACGNSVRNVTACPLA
                     GICHEESFDVTPYARSCSKFLMGHPDTQDFGRKFKIAFSGCKENPCGLVTMHDAGAIA
                     KIRVVDGAPQRGFEFYVGGGLGSVPYKAKLFDAFLPEEELLPILQAIARVYARLGEKR
                     NRARARIKFLIANLGIDEFRRQVLEEREKLPSDPRWTEFLSSVNEQAEQPLKAGIDLN
                     GHQRPDGFDAWYATNVYKQRQQGYVAATVSLPLGDASADQLRALADIARRFVKDTLRT
                     TVEQNILFRWVSEADLPDFYQAIRAIGLGAADAATIVDVTACPGTDTCKLGIASSRGL
                     ARELRYQLGEKSMEIDEAVKNLRIKVSGCFNSCGQHHVADIGFYGINRNVKGFQVPHF
                     QVILGGKFHDNAGEYGVAIGAVPSKRVPEAVSTITQYYVEKRLKDERFQDFVARIGKK
                     QIKELLDNLTKIPAHDVDRSYYSDWGDPREFTLADMGVGECAGEVVSQAEFALAASER
                     ELFEAQLLLDAGHNEPAAKVAYGSMVRAAQGLVKDQNPNVSDNENQIIAEFTRRFYDT
                     QLFWDKYAGGKFAEYFFKAKEFIASGKAPDADRAVQLLQEAQLFIDAAHDCHNKLRGP
                     APSAAAIAAAAQPSA"
     gene            2088..2708
                     /locus_tag="VFY96_12040"
     CDS             2088..2708
                     /locus_tag="VFY96_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607241.1"
                     /translation="MDLQKLTVKFFTAPPDSVPLTDFIEIFHGWIQATDGIYHDVADY
                     SHMQEGPGIVLVAQHANLHIDETSGRRGLLYCQKALLTGSNHDKLRTVLRAALKNCQR
                     LERDPALKGRIKFSGDEVLLSINDRLLAPNDAKTYQSLQPDLERAAAELFRPLKTTMT
                     HKQDARQRFAVRIRSFESLPIDMLLANLGPRKLDSMATGHGSRFST"
     gene            2846..3475
                     /locus_tag="VFY96_12045"
     CDS             2846..3475
                     /locus_tag="VFY96_12045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HEX5607242.1"
                     /translation="MGYFPIYLEMSGRRCLVIGGGTVAERKAAALLDAGAEVTVLSPD
                     VTENIADWLSQKAISVMPRYYQPGDLARFDMVFVATDDLQVNAAVYREGRSYGIWVNS
                     ADDPARCDFILPSVLRRGELTVAVSTAGHSPALARTIREELELYFTPEYESLAALAAE
                     VRKELLQRSVSVPFEAWRRALGGNVRQFLMRGDYARAKNQLLYELGVAE"
     gene            3472..4281
                     /gene="cobA"
                     /locus_tag="VFY96_12050"
     CDS             3472..4281
                     /gene="cobA"
                     /locus_tag="VFY96_12050"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013082137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEX5607243.1"
                     /translation="MTVGKVYLVGAGPGDPGLLTVRGLERLRSADVIVYDQLVNPAIL
                     AQASDRAARIYVGKQAGHHCAAQPDINRILIACARDGYEVVRLKGGDPFVFGRGGEEA
                     VALANAGISFEIVPGVSSAVAVPAYAAIPLTHRGVASSFAVVTGHPAGNTKSSVEWAK
                     LAGAVDTLVVLMGLKNLPHIVAELLAGGVSPRMPAAVIQQGTTTEQESVTGRLDNIVE
                     RTTHLKSPAVIVLGKVVELSEQLQWFFPESMLRESLTFHHEPLGNGLGVEA"
     gene            4530..5369
                     /locus_tag="VFY96_12055"
     CDS             4530..5369
                     /locus_tag="VFY96_12055"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX5607244.1"
                     /translation="MEASLVGVKVVSFESRRAKEIAELIRRYGGEPITAPAMREIPLS
                     ENHSALKMLPQLEAGQFDLLILMTGVGTRTLNELLLTKYSQEKIVAALRKARLVVRGP
                     KPVAALKELGLQPDITVPEPNTWREVLATVEQAVNLQGKRVALQEYGIPNPELVAGLQ
                     RRGASVETFAIYKWALPEDIGPLRAAIKKISNGEADVVLFTNGAQVDHLFQIAIEDGA
                     EEDLRRALKAIVIGSVGPVCTEVLHQFGVEPDVEPEHPKMGSLIAEIAASARDVLARK
                     GAR"
     gene            complement(5477..5899)
                     /locus_tag="VFY96_12060"
     CDS             complement(5477..5899)
                     /locus_tag="VFY96_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607245.1"
                     /translation="MRRCWREKIDAAIVGPGHVINATSSGVDLRATLPQTMLVTTRRL
                     AAAKPHVIEAYLKTFVEAIAVTLDPANKELVMRLQMTNLRLASSAAAEEAYHSVINSY
                     EREPHTSVEGMKRLQKLRRRSPRRSPMFGSNPQSILLS"
     gene            6185..>6627
                     /locus_tag="VFY96_12065"
     CDS             6185..>6627
                     /locus_tag="VFY96_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX5607246.1"
                     /translation="MTMIINNQEVEKVLTMPDTIAALEKSYLQLAAGEAVCRPRIDIR
                     IPTSDPARNYQWGTMEGGSTAGYFAIRMKSDIIYETEYNGAVTQEKYCIRPGLYCGLI
                     LLTSIENGELLAFINDGYLQHMRVGADGGIGVKYLANKDAEVVGML"
BASE COUNT         1475 a         1766 c         1978 g         1408 t
ORIGIN      
        1 caggattttc agcttcacta cgtccacatc gacgacacgc cggcgctgat gcgccgtctc
       61 gccgccgtga gcgtgaccag tcgcgaagcg tgcggcaatt cggttcgcaa cgtgacggct
      121 tgtccactgg cgggaatttg ccacgaagaa tcgttcgacg tcacacctta cgccagaagc
      181 tgttcaaagt ttctgatggg ccatccggac actcaggatt tcgggcgaaa gttcaaaatc
      241 gccttttccg gctgcaaaga aaatccgtgc gggctcgtga cgatgcatga cgcgggagcc
      301 atcgcgaaaa tcagagtggt cgacggcgcg ccgcagcggg gcttcgagtt ttacgtcggc
      361 ggcgggttgg gctcggtgcc ctacaaagcg aagctcttcg acgccttttt acccgaagaa
      421 gaattgctgc cgatcttgca ggcgattgca cgtgtttacg cgcggctcgg cgaaaaacgt
      481 aatcgggccc gcgcgcgaat aaaattttta atcgcgaatc tcggcatcga tgaatttcgc
      541 aggcaggtgc tcgaagagcg agagaagctg ccgtcggatc cgcgttggac agaatttttg
      601 tcatcggtca acgagcaagc ggagcagccg ctcaaggccg gcatcgatct caacggtcat
      661 cagcggccgg acggcttcga cgcgtggtac gcaaccaatg tctacaagca gcgccagcag
      721 ggctacgtcg cagcgacggt ttcgctgcca ttgggagatg cgagcgccga tcagctacgc
      781 gcgctcgctg acatcgcgcg gcgcttcgtc aaagacacgc tgcgcaccac cgtggagcaa
      841 aacattctct ttcgctgggt gagcgaagcg gatctgcccg acttttacca ggcgattagg
      901 gcgatcggtc tgggtgcggc ggatgcggca accatcgtcg acgtgacggc ttgcccgggc
      961 accgatactt gcaaattggg catcgcgtca tcgcgtggct tggcgcgcga gctacgctac
     1021 cagcttggtg aaaagagcat ggagatcgat gaggcggtga aaaatctccg catcaaagtc
     1081 agcggctgct tcaattcctg cggccagcat cacgtcgccg atatcggctt ttacggcatc
     1141 aaccgcaacg tgaaggggtt tcaggtgccg catttccaag tgattctcgg cggcaagttt
     1201 cacgataacg cgggcgagta cggtgtcgcc atcggcgccg tgccgtcgaa acgcgtgccc
     1261 gaggcggtct cgacgatcac tcagtattac gtcgaaaaaa gactcaagga cgagcgcttt
     1321 caagatttcg tcgcgcgcat cgggaaaaag cagatcaaag agctgctcga caatctaacc
     1381 aaaatccccg cccatgacgt ggatcgctct tactacagcg attggggcga tccccgcgag
     1441 ttcacgcttg cggacatggg ggtgggtgag tgcgccggcg aagtggtcag ccaggccgaa
     1501 tttgccttgg cggcgagcga gcgcgagttg ttcgaagcgc aactgctgtt ggatgcgggc
     1561 cataacgaac ccgcagcgaa ggttgcctat ggctctatgg tgcgcgccgc acagggtctt
     1621 gtgaaagacc agaatcccaa cgtttcagac aacgaaaacc agattatcgc ggaattcacc
     1681 cggcgctttt acgacacgca gcttttctgg gacaagtacg cgggcggaaa gttcgccgag
     1741 tattttttca aggcaaagga attcattgcc tccggcaaag caccggacgc ggaccgcgcg
     1801 gtgcagctac tgcaggaagc gcagctcttt atcgacgcgg cgcacgactg ccacaacaag
     1861 ctgcgcggtc cggcaccgag cgccgcggca atcgccgcgg ccgcgcagcc gagtgcgtga
     1921 ggagaagagg ggagaggaaa aaattttcct aaccctcttt tataaagagg ggaaccggag
     1981 aaccctccaa tggaacagga aaaagcttgg atatcacgga aggggagtga attggattaa
     2041 aggctgtttc ccttcgaaaa agggggagca aggggattta tcctcggatg gatctgcaaa
     2101 agctcactgt aaaatttttc actgcgccac cggatagcgt gccgctcacg gatttcatcg
     2161 aaatctttca cggatggatc caagcgacgg acgggattta tcacgacgta gccgattaca
     2221 gccacatgca ggaaggcccg gggatagtgt tggttgcgca gcatgccaat ttgcacatcg
     2281 acgagaccag cggcaggcgc ggtttgctgt actgccaaaa ggcgctgctc accggatcca
     2341 accacgataa gctccgcaca gtgcttcgcg ccgccctgaa gaattgtcaa cgtttggagc
     2401 gtgacccagc cctcaaagga agaattaaat tttccggcga tgaagttttg ctatccatca
     2461 acgatcgctt gctggcgcca aacgatgcga aaacctacca aagcttacaa ccggatttgg
     2521 aacgcgccgc tgccgaactg tttcgcccgc ttaagaccac aatgacccat aaacaggatg
     2581 cgcgacaacg gttcgccgtt cgcatcagaa gcttcgagtc gttgccgatt gatatgctgc
     2641 tcgcgaattt aggtccgaga aaattagata gcatggccac tggtcatggc tcacgatttt
     2701 cgacataggt gacgacagcg accagtgcga ttgagaagca cggccgcaaa aaatcttcta
     2761 ctgtttttga gattgtttca aatgtgcgac aaagctagat ttggaaagat actctgggaa
     2821 atcgacttcc gtagaaagga ttccgatggg ttactttcca atctatctcg aaatgagcgg
     2881 tcggcgttgt ctggtgatcg gcggcgggac cgtggcggag cgtaaagccg cagcgctact
     2941 cgacgcagga gccgaagtga cggtgcttag cccggatgtc acagaaaaca tcgccgattg
     3001 gttgagtcaa aaggcgatca gcgtaatgcc acgctactat cagcccggcg atttggcgcg
     3061 gttcgacatg gtttttgtcg ccaccgacga cttacaagtg aacgcggcgg tttatcgtga
     3121 gggcagaagc tacgggattt gggtcaactc ggcggacgat cccgcgcgct gcgatttcat
     3181 tttgccttcg gtgctccggc gcggtgaatt gacggtggcg gtttccacgg ccggtcatag
     3241 ccccgccttg gcacgcacca ttcgcgaaga gctcgagctt tacttcaccc ccgagtacga
     3301 gtctctcgcc gcgctggcgg cggaagtcag aaaagagttg ctgcagcgct cggtcagtgt
     3361 accgttcgag gcctggcggc gcgcgctcgg cggtaacgtg cgccagtttc tgatgcgcgg
     3421 cgattatgcc agggcgaaaa atcagttgct gtacgaattg ggagttgcag aatgaccgtc
     3481 ggaaaagttt atctagtcgg cgccggtccc ggagatccgg gactcttgac cgtacgtgga
     3541 ctcgagcggc tgcgctccgc ggatgtcatc gtctacgatc agctggtgaa tcctgcgatc
     3601 ctagcccaag cttctgatag ggcagcaaga atctacgtcg gcaaacaagc gggtcatcat
     3661 tgtgcggcgc aaccggacat caatcgtata ctcatcgcgt gcgctcgcga tggctatgaa
     3721 gtcgttcggc tgaagggcgg cgatccgttt gtcttcgggc gcggcggcga agaagcagtg
     3781 gcgctggcga acgcaggtat atctttcgaa atcgtccccg gcgtgagttc cgcggtcgcc
     3841 gtgccggcct acgcggctat accgctaacc catcgcggtg tcgcctcgtc ctttgcagtc
     3901 gtgaccggtc atccggcagg caatacgaaa tcatccgtcg aatgggcgaa gttggcgggc
     3961 gctgtggata cgctagtggt gttgatgggg ctcaaaaatc tgccgcacat agtcgccgag
     4021 ttgcttgctg gcggtgtttc tcctcggatg cccgcggcgg tgattcagca gggtacaacg
     4081 accgagcagg agagtgtcac tggaagactg gacaatattg tcgaacgaac tacgcacctg
     4141 aaatcgcccg cggtgatcgt gttgggcaaa gttgtcgaat tgagcgagca acttcaatgg
     4201 ttctttccag aatcgatgct gcgagagagt cttacttttc atcatgaacc gctcggaaat
     4261 gggctcggcg tcgaagcttg aagcagccgc aatgcggttg gactaagcga tttttaccac
     4321 ggagttattc atgagcttac gctcgcctac aaaatgaaaa gcggttgtac ttggatgttt
     4381 ctaattgcga ttcttcggcg aatccatcat tcccgcatgt tttagcggaa tgccgcgtgg
     4441 ggaattctga ctgacatcgg gttaatacat ttggcgcgct tgttggagta aaacgctttg
     4501 gagtttctta ccggaagcgg gagagtgcgg tggaagccag tctcgtcggc gtgaaggtgg
     4561 ttagcttcga gagccggcgc gccaaagaga ttgcggagct cattcgccgc tacggcggcg
     4621 agccgatcac cgcgcctgcc atgcgcgaga ttccgctgag cgaaaatcac tctgcgctca
     4681 aaatgctccc gcaacttgaa gcaggtcaat tcgatttgct cattctgatg actggcgtcg
     4741 gaacccggac tctcaacgag cttcttttaa cgaaatattc tcaagagaaa atcgtcgcag
     4801 ccttgcgtaa ggctcgtttg gtggtgcgcg ggccgaagcc agtcgccgcg ttgaaagaac
     4861 tgggattgca gcccgacatc acggtgccgg agccgaatac gtggcgcgaa gttcttgcca
     4921 ctgtagagca ggcggtcaat ctgcaaggaa agcgcgtcgc gctgcaagag tacgggattc
     4981 cgaatccgga gctggttgcc gggttgcagc ggcgcggcgc cagcgtcgaa acgtttgcaa
     5041 tctataagtg ggcgttaccc gaagacatcg gcccattacg cgcggcgata aaaaaaatat
     5101 cgaacggcga ggctgacgtg gtgctgttca cgaacggcgc acaagtcgat catctctttc
     5161 agatcgcaat cgaggacggc gcggaagaag atctccgccg cgcgctcaag gcaatcgtga
     5221 ttggatcggt cgggccagtc tgcactgaag tgctgcacca attcggagtt gagcccgatg
     5281 tcgagccgga gcatcccaaa atgggatcgt tgatcgccga gatcgccgca tccgcgcgcg
     5341 acgttctcgc gcgtaaaggc gcccgataga atcgactccc ttatgtagcg ccgataactt
     5401 gctgccaccg aaatctattt cgggagctcg ttgttcttgt aaaggctttg aatgtaaccc
     5461 gaggattcga gcttgttcat gaaagaagta tcgactgcgg gttcgacccg aacattggcg
     5521 atcttcgggg tgatctgcgc ctcagcttct gcagccgctt cattccttcg acgctagtgt
     5581 gtggctctcg ctcgtatgaa ttgatgaccg aatgatacgc ctcctccgca gcggctgaac
     5641 tggccaagcg cagattcgtc atttgcaatc gcatcaccaa ttctttgttc gcgggatcaa
     5701 gggtcaccgc gatcgcttcg acgaaagttt tcaagtatgc ctcgataacg tgaggtttcg
     5761 cggccgcaag ccggcgcgtc gtcaccagca tggtctgcgg caatgtcgcc ctgagatcca
     5821 caccgctgct ggtcgcattg atgacatggc cgggtccgac gattgctgca tcgattttct
     5881 cccgccagca gcgccgcaat tgcgagcggg ttgcgcggca tgagaatgaa ttgcatgtcg
     5941 aggccgtgtt tcttgaacaa accacggtcc cgtgcgaccc aaagcacggt catggtctca
     6001 ctggtgacgc tgccggctag gttggtgcgc gtctgagatc ggttgttata ttgtcaaggc
     6061 aaactcaggc gaaaccatcg ttgcgggtga ggcctttttt gctccgtgac acggcacgtg
     6121 taatcctgac agaaatatgc gatttctgag tcacggggga caaagcgagc tgaggtaaat
     6181 gcttatgacg atgatcatca ataaccagga agtcgagaaa gttcttacaa tgccggacac
     6241 cattgctgcg ctggaaaaat cctatctaca gctcgctgcc ggcgaggcgg tttgccggcc
     6301 gcgcatcgac attcgaatcc ccaccagcga tccggcgcgg aactatcaat ggggcacaat
     6361 ggagggagga tcgaccgcgg gctatttcgc catccgcatg aagtccgaca tcatctatga
     6421 aaccgaatat aacggcgccg ttacccagga gaagtactgt atacggcccg gactttactg
     6481 cgggctgatt cttttgactt cgatcgaaaa cggcgagttg ctggcgttta tcaacgacgg
     6541 ttacctgcag cacatgcgcg tcggcgcgga cggcggcatt ggcgtgaagt atctcgccaa
     6601 taaggatgca gaagtggtgg gtatgct
//
LOCUS       DASULZ010000584         3592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_981181, whole genome shotgun sequence.
ACCESSION   DASULZ010000584 DASULZ010000000
VERSION     DASULZ010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981181"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /gene="lpxB"
                     /locus_tag="VFY96_12070"
     CDS             complement(<1..1045)
                     /gene="lpxB"
                     /locus_tag="VFY96_12070"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEX5607247.1"
                     /translation="MAPKEIMLVVGEASGDVHGARFVEALHRCDPQVKVYGVAGEQLE
                     RSRFEPLFNVAQLTGMGLVELAGNLRNIWRAYALLKRTLRARRPNLLVLIDFPEFNLR
                     LARFAKSLGIPVLYYVSPQIWAWRKNRIKQIARWVDHMAVIFPFETDFYERQGVKVSF
                     VGHPLLEIVKPKTGRAETLTKLGLDPSRPAIALLPGSRRGEVTRHLPAMAEAALELSR
                     ERKAQFFCIRAATINDAELRSWLDRTGVQMPIVEDDRYGAIQASDLAWTASGTATVEI
                     ALLGRPMLIIYRVSWLTYMIARWLVRVDHIGMVNLIAGQRLMPELIQRDVNPERIAAE
                     TRILLDDTKMRGRI"
     gene            complement(1062..1997)
                     /locus_tag="VFY96_12075"
     CDS             complement(1062..1997)
                     /locus_tag="VFY96_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5607248.1"
                     /translation="MSGAENIIASGVVGVGYLGKFHAEKYASSSKAKLIGIVDINESR
                     VNEIGAALAAPAYTDYRNLFGRVQCVSIAVPTRLHYQIARDFIDAGIDVLVEKPLSAS
                     IAEARELVAIAEKKNVLLQVGHLERFNPAIRRLEGVIKEPKFVECHRLAPFVERGTDV
                     DVVLDLMIHDIDVIASLVRAPIERVEAVGVPVLTERPDIANARMRFANGCIANITASR
                     VSLKRERKIRFFQPDAYISIDYDQLRAQIFYKPAPGAGWLDIRTETVEIKEGDALADE
                     IDSFLECVRSRKHPLVGGAEGLRALEIASMISAQL"
     gene            complement(2000..2806)
                     /gene="lpxI"
                     /locus_tag="VFY96_12080"
     CDS             complement(2000..2806)
                     /gene="lpxI"
                     /locus_tag="VFY96_12080"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419874.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HEX5607249.1"
                     /translation="MKKIGLIAGNGKFPLIFAEQANRAGYNVVAVAHRGETDPAIERL
                     VKEIDWVYVGQLGKIIRCLQRAEVNEAVMVGGIRKVKLFDNFRPDLRGTRFLAKLKSR
                     EDDDLLRGIANELAADGIKVVESTFCLSEIIPAQGVLTKRSPRHQEWDDIQLGFRTAK
                     EIGRLGIGQTVVVKKQIVLAVEAAEGTDAAIERGGKLAGSGCVVVKVSKPQQDLRFDV
                     PAIGLETVNRLHEVRGRVLAIEAGKTILLEKDSLLKHADAYGISLVGVSS"
     gene            complement(2886..>3592)
                     /gene="lpxA"
                     /locus_tag="VFY96_12085"
     CDS             complement(2886..>3592)
                     /gene="lpxA"
                     /locus_tag="VFY96_12085"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEX5607250.1"
                     /translation="YCVIGAGARVGKGTRIASHVVIQGRTTLGEENVVFPFATVGTIP
                     QDLKYKGEASELLIGNRNTIREYVSLNPGTAGGGMVTRIGDQNLLMMQCHIAHDCIIG
                     NRNVIANGATLGGHVTIEDFVIVGGLVGIHQFVRIGTGAIIGAGSMVSKDVPPYCNAT
                     GDRAKLHGLNLEGLKRRGFDRRVIDIIRKAYRIVFQSKLRTDDALKKVSAELPGIPEI
                     ERFVSFISQSQRGVCR"
BASE COUNT          800 a         1052 c          945 g          795 t
ORIGIN      
        1 tgattctgcc tcgcattttg gtatcatcga gcaaaatcct agtttccgca gcgattcgtt
       61 ctgggtttac gtcgcgctgg atcagctccg gcatcaagcg ttgtccggcg atcagattca
      121 ccattccgat atgatcgacc cgcacaagcc aacgagcaat catataagtc agccaggaca
      181 ctcgataaat gatgagcatg ggccggccga gtagcgcgat ctccaccgtc gctgtgcccg
      241 acgccgtcca ggccaggtca gaagcctgga tcgcgccata acgatcgtct tcgacaatgg
      301 gcatctgcac accggttcga tcgagccacg agcgcagctc cgcgtcgttg atggtcgccg
      361 cccggatgca aaaaaattgc gctttacgct cgcgcgaaag ttcaagtgcg gcttcggcca
      421 tcgccggcag gtgccgcgtt acttcaccgc gacggcttcc gggtagcagc gcgatcgccg
      481 gccggctcgg atcaagccca agcttggtga gagtttccgc gcgcccggtt ttcggcttga
      541 caatttccaa cagtggatgg ccgacgaaac tgactttgac gccctggcgc tcataaaaat
      601 ccgtttcgaa aggaaaaatg accgccatgt ggtcgaccca tcgggcgatt tgtttaatcc
      661 gatttttgcg ccaagcccaa atctgagggc tgacataata aagcaccgga atcccaagtg
      721 acttggcaaa acgcgccaga cgcaagttaa attccggaaa atcgatcaac acgagaagat
      781 tggggcggcg cgctctcaaa gttcgtttca gcaatgcgta agcgcgccag atatttctca
      841 aattcccggc cagttccacc aaacccatgc cggtcaattg ggcgacattg aagagcggct
      901 caaatcgact ccgttctagt tgctcgcccg cgacaccgta cactttcact tgagggtcgc
      961 atcgatgcag tgcctccaca aaccgcgcgc catgcacatc accggaagct tcaccaacga
     1021 ccaacatgat ttctttcggc gccatcagac aaaaccgcac ctcagagctg cgcgctaatc
     1081 atcgatgcga tttccagggc gcgtagtccc tccgcgccgc cgaccaacgg atgcttacga
     1141 ctgcggacgc attcgagaaa agaatcgatt tcgtcggcta gagcatcgcc ttccttgatc
     1201 tccaccgtct cggtgcgaat gtcgagccag ccagcgccgg gagccggttt gtaaaaaatt
     1261 tgcgccctga gttggtcata atcgatcgaa atgtaggcgt caggctgaaa aaagcggatc
     1321 ttccgttccc gtttgagtga cacgcggctc gccgtgatgt tcgcaatgca accgttggcg
     1381 aatctcattc gagcattggc gatatccggc cgctcggtga gtacgggcac tcctaccgcc
     1441 tcgacgcgct cgatcggcgc gcgcaccagg ctggcgatta catcaatgtc atgaatcatc
     1501 aaatccagga ctacatccac atccgtgccg cgctcgacga agggcgccaa acgatggcat
     1561 tcgacgaact tgggttcttt aatgacccct tcgaggcggc gaattgcggg gttgaagcgt
     1621 tcgagatgac cgacctggag cagcacgttt ttcttctcgg cgatcgcaac gagctcgcgg
     1681 gcttcagcga tgctcgcgga tagcggtttt tccaccagca cgtcgatccc cgcatcgatg
     1741 aaatcccgcg cgatctgata atgaagccgg gtcggcacgg caatgctgac gcactgcacc
     1801 ctgccgaata aattccggta atccgtatac gccggcgctg ctagcgctgc gccaatttcg
     1861 ttgacgcgcg actcgttgat atcgacaatc cctatgagct ttgccttgga tgaagaggca
     1921 tatttttctg cgtgaaattt gccgagatag ccgacgccga ccacgccgct ggcgatgatg
     1981 ttttccgctc ccgacataac tagctgctca cgccgactaa tgaaatgccg tacgcatccg
     2041 cgtgtttcaa cagactgtcc ttttccagca gaatggtttt accggcttcg atggcgagca
     2101 ctcggccgcg aacttcgtgc aggcggttga cggtctccag gccgatcgca ggcacatcaa
     2161 agcgcaaatc ctgctgcggc ttgctgactt tgaccacgac acagcctgat ccggccagtt
     2221 ttcctccgcg ctcgatcgcg gcatcggttc cttcggcggc ttccactgcg aggacaattt
     2281 gcttcttgac caccaccgtc tggccgatgc ccaaacgtcc gatttccttt gcggtacgaa
     2341 aacccaattg aatatcgtcc cattcctggt ggcgtggcga acgcttggtg agaacaccct
     2401 gagcaggaat gatttcggag agacagaacg tcgattcaac aaccttaatc ccatccgcag
     2461 cgagttcgtt ggcaatgccg cgtagtaggt cgtcatcttc ccggcttttg agctttgcca
     2521 aaaaccgcgt gccccgaaga tcgggtcgga aattgtcaaa aagcttgacc ttgcgaatgc
     2581 cgccgaccat taccgcctcg ttcacttccg cccgctgcaa acagcggata atcttgccca
     2641 attggccgac gtacacccaa tcgatttctt tcactagccg ctcaatggca ggatcggttt
     2701 caccgcggtg cgcgacggcc acgacattgt agccggcacg attggcttgc tcggcgaaaa
     2761 tcaacggaaa cttaccgtta ccggcgatca aacctatttt tttcacttga tctgactgta
     2821 accgggagaa tccattcaga agaaaccaat tacttttagg cgggaatgat tcgactgcaa
     2881 agctgttagc ggcatacgcc tcgttgagat tggcttatga acgagacgaa tctttcaatc
     2941 tcgggaatac cgggcagctc cgcggaaacc tttttgagcg catcgtcggt tctaagcttg
     3001 gattggaaaa caatccggta ggccttgcgg atgatatcga tgacccttct gtcgaagccg
     3061 cggcgtttca ggccttcgag gttcaggccg tgcagtttgg cgcggtcgcc ggtcgcgtta
     3121 cagtagggcg gaacatcctt tgaaaccatc gatccagccc cgatgatcgc gccggtgccg
     3181 atccggacga attgatgaat gccgactaac ccacccacga tgacaaaatc ttcgatggtg
     3241 acgtggccgc cgagggtcgc accgttcgcg atgacattcc gattgccgat gatgcagtcg
     3301 tgggcgatgt gacactgcat catcaacagg ttttgatcgc cgatccgcgt gaccatgccg
     3361 ccgcccgcag tgccgggatt gagcgagaca tattcgcgaa tcgtattgcg attgccgatc
     3421 aagagctcgc tcgcttcacc cttgtattta agatcttgag gaatggtgcc gacggtagca
     3481 aaagggaaga ccacattttc ttcgcctaat gtcgtccggc cctgaataac gacatgagag
     3541 gcaattcttg ttcctttgcc aactcgggcg ccggcgccaa tcacacaata ag
//
LOCUS       DASULZ010000585         2417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_575824, whole genome shotgun sequence.
ACCESSION   DASULZ010000585 DASULZ010000000
VERSION     DASULZ010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575824"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="VFY96_12090"
     CDS             complement(<1..996)
                     /locus_tag="VFY96_12090"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX5607251.1"
                     /translation="MRDSVQRSNQDWLLLVGLFCFFFSGAAGLIYQVVWTRMLTQIFG
                     NTTYAIATVLSAFMAGLAIGSYLFGQIADRGKNDFLLYGILEAGVGVYGFLVPWIFKI
                     AQKIYGPIFGLNESLPFLFNLVLFFLSFVLLVFPTLLMGATLPVLSRFFVRSFAQFGR
                     RVGDLYATNTLGAVIGCAAGGFYLIPTLGMRETVYVAAGVNLLIAAVILIVDRFRDKA
                     PVEALVERPVEEPPAGEEQPTSALAWVVLGSFALSGFASLVYENAWTRSLTLVIGSSI
                     YSFSTMLVTFLIGLALGGFIYARMLGGREARLSTFGLIELWVGLSALATIPLFERL"
     gene            1284..>2417
                     /locus_tag="VFY96_12095"
     CDS             1284..>2417
                     /locus_tag="VFY96_12095"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX5607252.1"
                     /translation="MANAKRDGAAPEEVRDLRRAIEWMKQEGDILITDKEVDPDLEIT
                     GIQKQLDGGCPILFNNVKGKPHHRCITNLFGDMNVINKMFGWKDHADRTRKLAYAVTH
                     PIPPVVIDQKDAPVQEEVYEKPAEANDYVVPIRHTNVEPELTVGSGNRLISGEYFNGG
                     TDLGYNRMNFRWGNVGTFQISPGSHMWQVVSKYYKDNKMVPITMNFGLPPAGSLLAGA
                     GFDYVILPQGCDEVGMAGAVQGAPMRLVKARTIDAYALADAEVVLEGYVNPRDRRFET
                     AESEKAGVQGRFHFHPEWAGYMGKAYKAPTFHVTAVTTRKREGKPIIFPLGVHTLDDH
                     NIDTTVREAAMFELCERMQPGIIMDVNIPYCMTDWGGAIIQVKK"
BASE COUNT          632 a          668 c          669 g          448 t
ORIGIN      
        1 caatcgctcg aacaaaggaa tcgtcgcgag cgccgataat ccgacccaga gttcgatcaa
       61 gccgaaggtg ctcaggcgcg cttcccgccc gccgagcatt cgggcgtaga tgaagccgcc
      121 cagcgccaaa ccgatcagga acgtcaccag catcgtggag aaggaataga tcgagctgcc
      181 gatgacgagc gtcagggagc gcgtccaggc attttcataa accaaagaag cgaagccgga
      241 gagcgcaaac gaaccgagca cgacccaggc gagcgcggaa gtaggctgtt cctcgcccgc
      301 cggcggctct tcgaccggcc tttcgacgag agcctcgacc ggagctttgt cgcggaaccg
      361 atcgacgatc aaaatcaccg cggcgatcag cagattgact ccggccgcga catacacggt
      421 ctcacgcatt cctaacgtgg gaatcagata aaaaccgccc gcggcgcaac cgatgactgc
      481 accgagggta ttggtcgcat agagatcgcc gacgcggcgg ccaaattgcg cgaagctccg
      541 aacgaaaaac cggctcagca ccggcagggt tgcgcccatc agcaaagtcg gaaagaccaa
      601 aagcacgaat gatagaaaaa acagcacaag attgaatagg aacggcaggc tttcgttcaa
      661 accgaaaatc ggcccataaa ttttttgcgc gattttaaag atccacggca ccaaaaaacc
      721 gtagacgccg acgccggctt cgagaatgcc atagagtaag aaatcgtttt tgccgcgatc
      781 ggctatttga ccgaaaaggt aactgccgat cgcaagcccc gccatgaagg ccgacaaaac
      841 cgtcgcgatc gcataggtgg tgttgccgaa aatctgcgtc agcatgcgcg tccatactac
      901 ctggtaaatc aaacccgcgg cgccggaaaa aaagaagcag aacagtccga ccagcagcag
      961 ccagtcttga ttggagcgct gaacgctatc gcgcatatat tctcccaaat cctcgaaaaa
     1021 acaattcaaa gagtgatagc aagccgctgg taaactgtaa agagtaatgc cgcgctggta
     1081 ctccgctgtt tcgtttcgtc gcgctgttcg ttgactttgc taggattgtc gtataatcgt
     1141 accttcgttt tggcgtcccg aatctgacaa ttctcccctg tatcgttgat cgaacaattg
     1201 aagtgaccat gggccgatcg taaaattttc gttttgacga agcggcgtag ctacgacccg
     1261 atcaaaggga tttggaggaa gcaatggcaa acgcgaagcg agacggtgcc gcccccgaag
     1321 aagtgagaga tttgcggcgt gctatcgaat ggatgaaaca agaaggtgat atcctcatca
     1381 ccgacaagga agtcgatccg gatctcgaga tcaccgggat tcagaaacaa ctcgatggcg
     1441 ggtgtccgat cctgtttaac aatgttaaag ggaagccgca ccatcgctgc attaccaacc
     1501 tgttcggcga tatgaacgtc atcaataaaa tgttcggctg gaaagatcat gccgatcgga
     1561 ccagaaaatt agcttatgcc gttacgcacc cgatcccgcc cgtggttatc gatcagaagg
     1621 acgcccccgt gcaggaagag gtgtacgaga aacccgccga ggcgaatgat tacgtcgtgc
     1681 cgatccgcca taccaatgtc gagcccgagt taacggtcgg ttccggtaat cggctcatct
     1741 cgggagaata cttcaacggc ggcaccgacc tcggttacaa ccgcatgaac tttcggtggg
     1801 gaaatgtcgg cacgtttcaa atctcgccgg gttcccacat gtggcaggtc gtaagcaaat
     1861 actacaaaga caataaaatg gtgccgatca cgatgaactt tggtctgccg cccgccggat
     1921 cactgctcgc cggagcggga ttcgattatg tgatcctgcc ccagggctgc gacgaagtcg
     1981 gcatggctgg cgcggtgcag ggcgcgccga tgcggctggt gaaagcgcgc acgatcgacg
     2041 cctacgcttt ggccgatgct gaagtcgtgc tcgagggata cgtgaacccg cgcgaccgcc
     2101 gctttgaaac cgcggaatcg gaaaaggccg gcgtacaagg ccgtttccat tttcatccgg
     2161 aatgggccgg ttacatgggc aaggcctaca aagcgccgac cttccatgtg accgccgtca
     2221 ccacccgtaa acgtgaaggc aaaccgatca tcttcccgct cggtgtgcac accctcgacg
     2281 accacaacat cgataccacg gtgcgcgaag cagcgatgtt cgagctttgc gagagaatgc
     2341 agccgggcat tatcatggac gtgaacattc cttattgcat gaccgactgg ggcggagcga
     2401 tcattcaggt caagaag
//
LOCUS       DASULZ010000586         4233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_68885, whole genome shotgun sequence.
ACCESSION   DASULZ010000586 DASULZ010000000
VERSION     DASULZ010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68885"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFY96_12100"
     CDS             <1..345
                     /locus_tag="VFY96_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX5607253.1"
                     /translation="VDGRSVGDVGDVVIRDRRHLSEDGMVLVVMAIHQQSGELVAGPD
                     LISRGFMGAEESEEVLENAKKVVLETLAASNRETRTDPAELKEEVRRSLRRFFRKKLE
                     RHPVVLPYIMET"
     gene            342..2612
                     /locus_tag="VFY96_12105"
     CDS             342..2612
                     /locus_tag="VFY96_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX5607254.1"
                     /translation="MRLSTRSSAPEKNRLQREIWGVVICLVALLITLSLISYDPSDRS
                     LNTPSGAINTHNWGGFIGAFLADLLLQSLGITAYLIPVLLCLAAIRMFRTNYKGVQLT
                     KALAYAVLLLSLGVILSVAIDTESARDAGGIIGGFLNERVLVPLFGRLSAILIACFTM
                     LLSVMVLTQNSLLDLLGHTKKSFVEFRKSFVPAVSNRLKEFKEKTDKRKGENTKKERK
                     DYVPPPIVLKEEVKDDPVARKSPKKPTPPEQFKLPEVGEGYKLPPLELLDPPEGEQFK
                     IDKETLHANSLILQKKLEDFGVEGEVVAVRPGPVITMYEFKPAPGVKVRRIVMLADDL
                     AMALRAVSVRILAPIPGESVVGIEIPNPRRETVYLREVIESEAYRNTDSKVALAFGKD
                     IGGTPFATDLAKMPHLLVAGATGTGKSVSINAMILSILFKSTPQDVKFIMVDPKMLEL
                     TVYEEIPHLLVPVVTDPKKAAAALFWAMDEMDRRYRLMRDKGARNIDNYNRTLEREAG
                     RKAVIDLTEPEWSGETLSPGGNLAQDAPLVHERLPRIVIIIDELADLMMTVGRDIEEY
                     ITRLAQKARAAGIHLILATQRPSVDVITGLIKANFPARISFQVTSRVDSRTILDSMGG
                     EKLLGNGDLLFLPPGTARLIRVHGAFVSDQEVRRVMKFIKQQGRPNYRPEVLEAKRET
                     EAAAAAEDEYDEMYDQAVAIVTETQQASISMIQRRLRVGYNRAARMIEQMERDRVVGP
                     ADGAKPREVYARKIEP"
     gene            2602..3273
                     /gene="lolA"
                     /locus_tag="VFY96_12110"
     CDS             2602..3273
                     /gene="lolA"
                     /locus_tag="VFY96_12110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEX5607255.1"
                     /translation="MNLSRAIGLIASVFLVAIALTAKASVAADSKNAEAIVDSLQKNY
                     DTTTDFTADFQQETEVKNINRTLKASGKLSFKRPGKMLWRYDEPKGQFVIADGKYLYF
                     YQPDQNQVIKSPLKNAFRGDIPLSFLLGLGNLKKDFNATRQSSDGNLYVLRLEPKGEA
                     GGFSEILLAVNKGSFDIVWVSVHDAANNVTTLRFSNMRKGVGVKDSLFQVQIPNGADV
                     VELGT"
     gene            3274..>4233
                     /gene="rimO"
                     /locus_tag="VFY96_12115"
     CDS             3274..>4233
                     /gene="rimO"
                     /locus_tag="VFY96_12115"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX5607256.1"
                     /translation="MAQRQKFNIISLGCARNLVDSEVMAGVLHQNDYELVPAPEAADI
                     VLINTCGFIAAAKEESIDTILDVARLKEAGKLKKLVVAGCLSQRYPNELAKELPEVDL
                     FIGTGEVPRIAQILKEQEAHARRQFIGLPSYLYDHSTPRVLSTPSYTAFVKVSEGCDH
                     KCAFCIIPQMRGPHRSRSIESIVSEICKLTEDGVKEINLIAQDLTAYGRDRKDRTTLE
                     ALLRELANVPKLHWLRLLYAYPNFLDDTLLEVIRDSEKICKYIDIPFQHISQSILRRM
                     RRGKSGSSVREAAQKLRKFIPGLTLRTSLIVGFPGETDADFSEL"
BASE COUNT         1024 a         1136 c         1154 g          919 t
ORIGIN      
        1 gtggatggca gaagcgttgg agatgtcggc gatgtcgtca tccgcgaccg tcgccacttg
       61 tcggaagacg gcatggtgct cgtcgtcatg gcgattcacc agcagtccgg cgaattggtc
      121 gcgggtcccg atttgatctc ccgaggattc atgggcgcgg aagaaagcga agaggtttta
      181 gagaatgcca agaaggtggt gctcgagact ttggccgcca gcaatcggga aactcgcacg
      241 gatccggcgg agttaaaaga agaagtgcgg aggtcgctgc gacgcttttt taggaaaaaa
      301 ctcgagcgcc atccggtggt gcttccttac atcatggaaa cctgaggtta tccaccaggt
      361 ccagtgctcc cgaaaaaaac cggctccaac gcgaaatttg gggcgtggtc atctgtctcg
      421 ttgctttgct gataacgctc agtttgatct cctacgaccc gagcgatcgc tcgctcaata
      481 ctccatccgg ggccatcaat acccataact ggggcggctt catcggcgca tttctcgccg
      541 atctactgct acaaagtctc ggcattacgg cctatctaat tccggttttg ctttgtctgg
      601 cggcaattcg aatgtttcgc accaactata aaggtgtaca gctcaccaaa gccttagctt
      661 acgcggtcct gcttttgagc cttggggtca ttttgagcgt ggcgatcgac accgagagcg
      721 cgcgcgacgc cggcggcatc atcggcggtt ttctcaatga gcgagttttg gttcctcttt
      781 tcggcaggct gagcgccata ttgatcgcct gtttcacgat gctcttgtcc gtcatggtgc
      841 tgacccaaaa ctcattgctc gacctgctcg gacataccaa gaaaagtttc gtcgagttca
      901 ggaagtcttt tgtgcccgcg gtgagcaacc ggctgaagga gttcaaagaa aaaaccgaca
      961 agcgcaaagg tgaaaacacc aagaaagaaa gaaaagacta tgttcccccg ccgatagttc
     1021 tgaaagaaga agtcaaagac gatccggtcg cgagaaaatc accaaaaaaa ccaacgccgc
     1081 ccgagcaatt caagctgccg gaagttggcg agggctacaa gctgccgccc ctcgagctct
     1141 tggatccgcc ggagggcgag caattcaaga tcgacaaaga aacgcttcac gccaattcac
     1201 ttattctgca aaagaaactc gaagatttcg gcgtcgaggg cgaagtcgtc gcggtgcgtc
     1261 cggggccggt gatcaccatg tatgaattca aaccggctcc aggtgtcaaa gttcggcgca
     1321 tcgtcatgct tgctgatgat ctggcgatgg cgctccgcgc ggtgagcgtg cgcattttgg
     1381 cgccaattcc cggcgagtcg gtggtcggga tcgaaattcc caatccgcgg cgcgaaacgg
     1441 tttatcttcg cgaagtgatt gaaagcgaag cctaccgtaa caccgattcc aaggttgccc
     1501 tcgccttcgg caaagacatt ggcggaactc cgttcgcaac ggacctcgcc aagatgcccc
     1561 atctgttagt cgcgggagcg accggaacgg gcaaatcggt ttcgatcaac gcgatgatcc
     1621 tgagcatcct attcaaatcg acgccgcagg acgtcaaatt catcatggtc gatccaaaga
     1681 tgctcgagct gacggtttac gaggaaattc cgcacctgtt ggttccggtc gtcaccgatc
     1741 caaaaaaagc cgccgctgcg ctgttttggg cgatggatga aatggatcgg cgctaccggc
     1801 tgatgagaga taagggcgcg cgcaacatag acaattacaa tcgcaccttg gaaagagaag
     1861 ccggaagaaa ggcagtcatt gacctgaccg aaccagaatg gtctggagaa accctcagcc
     1921 ccggcggtaa tctcgcgcag gacgcgcctc ttgtccacga aaggctcccc agaatcgtta
     1981 tcattattga cgagcttgca gacctcatga tgacggttgg acgcgatatt gaggaataca
     2041 tcacccggtt ggcgcaaaag gcgcgcgccg ccggaattca cttgattctc gcgacgcaaa
     2101 gaccctccgt cgacgtcatt accggattga tcaaggcgaa ctttccagcc cggatatcgt
     2161 ttcaggtcac ctcgagggtc gattcacgaa cgattctgga ttccatgggc ggcgaaaagc
     2221 tcttgggcaa cggcgatttg ctgtttcttc cccccggcac cgcccgtttg atccgcgtgc
     2281 acggcgcttt cgtttccgat caagaagtgc gccgcgtgat gaaatttatc aagcaacagg
     2341 gccggccgaa ttaccggcct gaagttctcg aggccaagcg cgaaaccgag gccgctgcgg
     2401 ccgctgaaga tgaatacgat gagatgtacg atcaggccgt ggcgattgtc acggaaactc
     2461 agcaagcatc gatctctatg attcagcgcc gattgcgggt aggctacaat cgtgcggctc
     2521 gcatgattga gcaaatggaa agggatcgcg tcgttggccc ggccgatggc gcaaagcctc
     2581 gggaagttta tgcgcgaaag attgaacctt agccgtgcga tcgggctcat tgcctccgtt
     2641 tttctcgtcg ctatagcact caccgcaaag gcctcggttg ccgcggatag caagaacgct
     2701 gaagcgatcg tcgatagcct gcaaaaaaac tatgatacga cgacggattt caccgccgat
     2761 tttcagcagg aaactgaagt taagaacatt aatcgcaccc tcaaggcgtc ggggaagctc
     2821 tccttcaaac gtccggggaa aatgctctgg cgttacgacg agcccaaagg gcagttcgtg
     2881 atcgccgacg gaaaatacct ttacttttat caaccggatc aaaatcaggt gatcaaaagt
     2941 cccttgaaaa acgccttccg cggcgatatt ccgctgtctt ttcttctcgg tcttggcaac
     3001 ttgaaaaagg atttcaatgc cacacgccaa tcgagtgacg gtaatcttta tgtcttacga
     3061 ttggaaccca agggcgaggc cggcggattt agcgagatct tactcgcggt caacaaaggg
     3121 tcctttgata tcgtatgggt aagcgtgcac gatgccgcga acaacgtgac caccctgcgt
     3181 ttttccaaca tgcgcaaagg cgtcggtgtc aaagactcgc tgtttcaggt acagattccc
     3241 aacggcgccg acgttgttga actggggact tgaatggctc aaaggcagaa attcaacatc
     3301 atcagtttgg gctgtgcgcg aaatctcgtg gactcggagg tcatggcggg agtgctgcac
     3361 cagaacgatt acgagttggt gccagcgccc gaagcagccg atatcgttct gatcaatact
     3421 tgcgggttta tcgcggcagc caaagaagaa tctatcgaca cgattcttga tgttgcgcga
     3481 ctcaaggaag caggaaaatt aaagaaactg gtcgtcgccg gctgcttgtc acagcgttat
     3541 ccgaatgagc tggccaagga gttgcctgaa gtcgacttgt tcatcggcac cggcgaagta
     3601 cctcgaatcg cgcaaatctt aaaagaacag gaggcgcacg ctcgccgaca attcattggc
     3661 cttcccagtt atctctacga ccatagcacg ccacgtgtcc tatcgacccc ttcctacacc
     3721 gccttcgtca aggtttccga aggatgcgat cacaaatgcg ccttctgcat cattccgcaa
     3781 atgcgcggcc cccaccgcag ccgatcgatc gagtcgatcg tcagcgaaat ttgcaaactc
     3841 actgaggacg gcgtcaagga aatcaacctg attgctcagg atctcaccgc atatggcaga
     3901 gaccgaaaag accgcaccac cctcgaagcc ttgcttcgcg agttggcgaa cgtcccgaag
     3961 cttcactggc ttcggcttct ctatgcttac cctaactttc tcgacgatac gctgctggag
     4021 gtgatccggg atagcgagaa aatctgcaaa tatatagaca ttccgtttca gcatatcagt
     4081 cagtcgattt tgcggcgtat gcggcgtggg aagagcggca gctcggtgcg ggaggcggcg
     4141 caaaagctca ggaaattcat cccaggattg acccttcgaa catcgttgat tgtcgggttt
     4201 cccggtgaaa ccgacgctga ttttagcgaa ctc
//
LOCUS       DASULZ010000587         5524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_676962, whole genome shotgun sequence.
ACCESSION   DASULZ010000587 DASULZ010000000
VERSION     DASULZ010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5524
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676962"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /gene="trmD"
                     /locus_tag="VFY96_12120"
     CDS             complement(<1..546)
                     /gene="trmD"
                     /locus_tag="VFY96_12120"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX5607257.1"
                     /translation="MLHFTVISIFPRMFDSALSHSILKRAQEKNLVKIELLDVRDYTS
                     DRHRTTDDTPYGGGQGMVMKPEPLVSAIDHARNNAPGARVILLSPRGSVFDQAKARRL
                     SREKNLVLICGRYEGVDERVSAFVDEEISIGDYTLSGGEPAAMVVIDAVARLVPGVLG
                     NANSAAEESFSNGLLEYPQYTR"
     gene            complement(561..1073)
                     /gene="rimM"
                     /locus_tag="VFY96_12125"
     CDS             complement(561..1073)
                     /gene="rimM"
                     /locus_tag="VFY96_12125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX5607258.1"
                     /translation="MDEPLVPLGEIVATHGLLGWLKLNPFNPETTALKPGAEVFIDQA
                     RTQTALLLEASSPHKRQLLIKLHQVDSIDAAARYVGTTLSVPESALEVLAPGEYYHYQ
                     IIGFEVFTTGGERVGIVESTIATAGGELYLVQSGSKEHLIPAVKEIIDKIDFANRTIT
                     IDPPAGLLDL"
     gene            complement(1093..1329)
                     /locus_tag="VFY96_12130"
     CDS             complement(1093..1329)
                     /locus_tag="VFY96_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013011544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX5607259.1"
                     /translation="MKELIEYVAKSLVSNPAAVTINETNVDGASLFELKVAKEDLGRI
                     IGKQGRTAKSLRTLLNAAASIANRKVVLEIVEEK"
     gene            complement(1337..1582)
                     /gene="rpsP"
                     /locus_tag="VFY96_12135"
     CDS             complement(1337..1582)
                     /gene="rpsP"
                     /locus_tag="VFY96_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013497166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX5607260.1"
                     /translation="MSVTIRMSRHGAKKKPFYRIVVSDQRFPRDGRYIEQVGTYDPRA
                     KSDAVKLDHEKINSWIKKGAKPSQTVSELIKRNQTAS"
     gene            complement(1602..2942)
                     /gene="ffh"
                     /locus_tag="VFY96_12140"
     CDS             complement(1602..2942)
                     /gene="ffh"
                     /locus_tag="VFY96_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX5607261.1"
                     /translation="MFESLTDKLELTFKRLRGQGKITEKNIDDALREVRLALLEADVH
                     VKVVKTFLDSVKAKSMGQEVLQSLTPEQQFIKIVRDELIGLLGGDYRDLDLKAAPPVV
                     IMLVGLQGSGKTTTVAKLARYLKKEKKRSPYLVPADVYRPAAIEQLKILAGELQLPVY
                     DANPEKSPVAICQQALEEAKRKFCDLLLIDTAGRLHIDEELMHELTAIKDAVQPHQIL
                     FVADSMTGQDAVNQAIGFDAKLGLSGIVLTKLDGDARGGAALSIRDMVGKPILFSGIG
                     EKLDALEPFYPDRMASRILGMGDVLSLIDKVQQNVEQKEAARLQQAFQKQQFTLEEFQ
                     LQLQQIKRMGPVGGLLEMIPGGKKLASQVDPGQAEKELKRVEAIINSMTVKERRNPAI
                     LNGSRRRRIAQGSGTTVTDVNRLMKQFLEMKKMMQRVSKLGMRSLLNRVPNPFN"
     gene            complement(3086..4744)
                     /gene="groL"
                     /locus_tag="VFY96_12145"
     CDS             complement(3086..4744)
                     /gene="groL"
                     /locus_tag="VFY96_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528688.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX5607262.1"
                     /translation="MAAKMVKFNRDARDAILKGVNILADAVTVTLGPKGRNVVLDKSF
                     GAPNVTKDGVTVAKEVELEDKFENMGAQMVKEVASKTSDVAGDGTTTATVLARSIFAE
                     GVKLVAAGHDPMTLKRGIDKAVEAIVGELKGMSKATKDPKEIAQVGTIAANNDSTIGD
                     VIAEAMNKVGKEGVITVEEAKGLETTLDVVEGMQFDRGYLSPYFVTDPERMECVLEDV
                     YLLIHEKKISNMKEILSILEQIAKTGKPFIIIAEDIEGEALATLVVNKIRGTLRCVAV
                     KAPGFGDRRKAMLEDIAILTGGKLIAEELGIKLDNINLQDLGRAKRIVVDKDNTTIVD
                     GAGKKSDLEGRIKQIRAQIDETTSDYDREKLQERLAKLIGGVAVINVGAATEVEMKEK
                     KARVEDALHATRAAVEEGIVPGGGVALLRSAVALEKLRVSGDERWGVNIIKRASEEPL
                     RRIATNAGVEGSIALQKVKEGKGAFGFNAADEEYEDLLKAGIIDATKVVRTALQNAAS
                     VASMLLTTEAMVAEKPEDKPAMPPMPPGGGMGGMGGMGGMGGMM"
     gene            complement(4772..5062)
                     /gene="groES"
                     /locus_tag="VFY96_12150"
     CDS             complement(4772..5062)
                     /gene="groES"
                     /locus_tag="VFY96_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX5607263.1"
                     /translation="MKIRPLQDRVIVKRVDEEEKSKGGIIIPDTAKEKPQEGKVVAVG
                     KGKPGDDGKITPLDVKVNDRILFGKYSGSEINFDGEEHLIMREEDILGVIEK"
     gene            complement(5245..>5524)
                     /locus_tag="VFY96_12155"
     CDS             complement(5245..>5524)
                     /locus_tag="VFY96_12155"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HEX5607264.1"
                     /translation="YIMRLSEGRISHLAHLIMSGLSQPRLAEFPNERRALLETKEALN
                     DFFRREDQIDDIVRHKISSLSRHVPPGSREWDILYRKYFEEESRKQRR"
BASE COUNT         1115 a         1549 c         1465 g         1395 t
ORIGIN      
        1 ccgcgtgtac tgcgggtact cgagcagccc gttactgaac gactcctcgg cggcagaatt
       61 cgcgttgccg aggaccccgg ggacgaggcg agcgacggca tcaatgacca ccatcgccgc
      121 gggctcgccg ccgcttaatg tgtaatcgcc gatcgagatt tcttcatcga cgaaggcgct
      181 gaccctttcg tcgacacctt cgtaacggcc gcagatcaac accagatttt tttcccgaga
      241 aagtcgtcgg gccttcgctt ggtcgaacac cgaaccacgt ggcgaaagaa gaatgacccg
      301 cgcacccggg gcattgtttc gagcgtgatc aatggcactg accagcggtt cgggtttcat
      361 gaccatgcct tggccgccgc cgtaaggcgt atcatcggtg gtgcgatgcc gatcgctggt
      421 atagtcgcgc acatcgagca gctcgatctt caccagattc ttttcctgcg ctcgcttcaa
      481 gatgctgtgg ctcagcgcgg aatcgaacat tcgaggaaaa atcgaaatga cggtgaaatg
      541 cagcatgcga ggcggcttta tcaaagatca agcaatcccg cgggtggatc gatggtgatt
      601 gtacgattgg cgaaatcgat tttgtcgatg atttctttga ccgcagggat caggtgttct
      661 ttgctcccgc tttgcacgag gtagagctcg ccgccggcag tggcgatggt agactccacg
      721 atcccgacgc gctcaccacc cgtggtgaaa acctcgaaac cgatgatttg atagtgatag
      781 tactccccgg gcgcgagcac ttcgagagcg ctttccggga ccgagagggt tgtgccgaca
      841 taccgcgcgg cggcatcgat gctgtccacc tggtgtagtt tgatcagcaa ctggcgcttg
      901 tgcggactgc tcgcttcgag cagaagagcc gtttgcgtgc gcgcttggtc gatgaaaact
      961 tcggccccgg gtttgagcgc tgtggtctca ggattgaaag gattgagctt caaccagccg
     1021 aggaggccgt gggtagcgac gatttcaccg aggggaacta gcggttcgtc catcggtact
     1081 tgcgccagct aattattttt cttcgacgat ttcgagaacg actttgcggt tggcgatcga
     1141 cgccgcagcg ttcagcaggg tgcggagcga tttcgccgtg cggccttgtt tcccgatgat
     1201 gcgcccgaga tcttccttgg cgaccttcag ctcgaatagg gatgcgccat ccacgttggt
     1261 ttcattgatc gttaccgctg caggattgct gaccaaagac ttggctacgt actcgatcag
     1321 ttctttcatc ggaagatcag ctggctgttt ggttgcgctt gatcaattcc gaaacggttt
     1381 gtgacggctt ggcgcccttt ttgatccagg aattgatctt ctcgtgatcg agtttgacgg
     1441 catcggattt ggcccgcgga tcgtaagttc caacttgttc gatgtagcgc ccgtcgcgcg
     1501 gaaagcgctg gtcgctaaca acaatgcgat aaaatggttt tttctttgcg ccgtggcggc
     1561 tcatgcgaat ggtgacgctc atgctaagtc ttcctctcct cttaattgaa agggttcggc
     1621 acccggttga gaagcgagcg catgcccagc ttgctcaccc gttgcatcat ttttttcatt
     1681 tccagaaatt gtttcatcag gcggttcacg tcggtaacgg tggtgccgct cccttgggcg
     1741 atgcgacggc gccgactgcc gttcagtatt gccggattgc gccgttcttt gacagtcatt
     1801 gagttgatga tcgcttccac tcgctttaac tctttctcgg cctggcccgg gtcaacttgg
     1861 gaagcgagct tttttccgcc cggaatcatc tcgagcagcc cgccaaccgg gcccatgcgc
     1921 ttgatctgct ggagctgaag ttggaactcc tccaacgtga attgctgttt ctgaaatgcc
     1981 tgctgcagac gcgccgcttc cttctgctcg acattctgct ggactttgtc gatcaatgag
     2041 agcacgtcgc ccatgcccaa aatgcgcgac gccatacggt cgggataaaa cggctccaaa
     2101 gcgtcgagct tttcgccaat gccggaaaac aggatgggtt tgccgaccat gtcgcggatc
     2161 gacaacgcag caccgccgcg cgcgtcgcca tcgagtttcg tcagaacgat tccggatagt
     2221 ccgagcttgg catcaaagcc gatggcctga ttcacggcgt cctgtcccgt catggaatcg
     2281 gccacaaaga gaatttgatg gggctgcaca gcgtccttaa tggcggtcag ctcgtgcatc
     2341 aactcttcgt cgatatgcaa acgtccggca gtatcgatca aaagaagatc acagaatttt
     2401 cgcttggcct cttctagcgc ttgttggcaa atggctacgg gcgacttttc cggattggca
     2461 tcataaaccg gcagctggag ctcgccggcg aggattttca attgctcgat ggccgcgggg
     2521 cgatacacgt cggcagggac caggtacggg cttcgcttct tttctttttt cagataacgg
     2581 gcgagcttgg caaccgtggt tgttttgcca gagccctgca agccaacgag catgatcacc
     2641 actggaggcg ccgcttttag atcaagatcg cgataatcgc cgccaagcaa gccgatgagc
     2701 tcgtcgcgca cgattttgat gaactgctgt tcaggggtga ggctttgcaa gacctcctgc
     2761 cccatggatt ttgccttcac ggaatcgaga aaagtcttga cgactttcac atggacatcg
     2821 gcttcgagga gcgccaggcg gacctcgcga agggcatcat cgatgttctt ttcggtgatc
     2881 ttgccctggc ccctcagccg cttgaaggtg agctcaagct tatcggttaa ggattcaaac
     2941 atgctcgcta aactgtgata ttttaaagga aaagttcgaa ccagggtaat atgaaaaaaa
     3001 cctggagtca agctcacggc aaacagaaag gcccgtcgcg cgcggttgca cgcgacgggc
     3061 cttggttatt tgtcagaaag ttgctttaca tcatgccgcc cattccaccc ataccgccca
     3121 taccacccat tccacctcca ggaggcatcg gcggcatggc cggcttgtct tccggtttct
     3181 cggctaccat ggcttccgtg gtcagcagca tgcttgctac tgatgcagca ttttgcagcg
     3241 cggtccgaac aaccttggtc gcatcaatga tgcccgcctt caggagatct tcgtattctt
     3301 cgtctgcggc attgaaaccg aaggcgcctt tgccttcttt gaccttttgc aatgcgatcg
     3361 agccctcgac gccagcgttg gtggcgatgc gtcgaagggg ctcttccgaa gcgcgtttga
     3421 tgatgttgac accccagcgc tcgtccccgg acacacgcag tttttccagc gctactgccg
     3481 atcggagcaa tgcgactccg ccgccgggaa cgatgccttc ttccaccgcg gcgcgggtgg
     3541 cgtgaagggc gtcctcgacg cgcgccttct tttccttcat ttccacttcc gtagctgcgc
     3601 cgacattaat cacggcgaca ccgccgatca gtttggcgag ccgctcctgc agtttctcgc
     3661 ggtcatagtc ggatgtggtc tcatcaatct gggcgcgaat ctgtttgatg cggccctcga
     3721 gatcggattt tttgccggcg ccgtcgacga tcgtcgtatt gtctttgtcg acgacgattc
     3781 gttttgcgcg gccaagatcc tgcaagttga tgttatcaag cttgatccca agctcttcgg
     3841 cgatgagttt accgccggtc agaatggcga tatcctcgag catcgctttg cggcgatccc
     3901 cgaaaccggg agccttcacg gcgacgcacc ggagcgtgcc gcgaatcttg ttgacgacca
     3961 aggtggccaa tgcttcgcct tcaatatctt cggcgataat gatgaagggc ttgccggtct
     4021 tcgcgatttg ctccaggatc gacaaaattt ccttcatgtt gctgattttt ttctcatgaa
     4081 tcagaagata aacgtcctcg agcacgcact ccattcgttc cggatcggta acgaaatagg
     4141 gcgagagata gcctcggtcg aactgcatac cttcaacgac gtccaaggtc gtctccagtc
     4201 ctttggcttc ctccaccgtg atgacacctt ctttgccgac cttgttcatg gcttcggcaa
     4261 tgacatcgcc aatcgtggag tcgttgttcg cggcgatcgt gccgacttga gcgatttctt
     4321 ttggatcctt cgtggctttc gacatcccct tgagttcgcc gacgatcgct tcgacggctt
     4381 tgtcaatacc gcgcttgagc gtcatgggat cgtgacccgc tgcaaccagc tttacgcctt
     4441 cggcaaaaat tgaacgcgcc aaaaccgtcg cggtcgtagt cccatcaccg gctacgtcag
     4501 aggtcttgct cgcgacttcc ttcaccatct gagcgcccat attttcaaac ttgtcctcga
     4561 gttcgacttc tttggccacg gtgacgccat ctttcgtgac attgggggca ccaaaggact
     4621 tgtccagcac aacgtttctt cccttcggtc ccagcgtaac cgtcacggcg tcggcgagaa
     4681 tattgacgcc cttcagaatc gcgtcacgcg catcgcgatt aaatttaacc attttagctg
     4741 ccatcgttcc ctcctaaatt ttccgaaata attacttttc gatcacgccg aggatatcct
     4801 cctcgcgcat aatcaggtgt tcttcgccgt cgaaattgat ctcactcccc gagtacttgc
     4861 cgaaaagaat gcggtcgttg accttgacgt caaggggagt gatcttgcca tcatcaccgg
     4921 gttttccttt ccccacagcc actactttgc cttcctgcgg tttttccttt gcagtatcag
     4981 gaataataat tccccctttg cttttctcct cttcgtccac tcgcttgaca attacccggt
     5041 cttgtaaagg cctaatcttc actgcattcc cctttcgaag taggatgtaa gtatctgaat
     5101 ttttgaagga tttacccggg ctctagctag tgaataacca taagccaagg caaaacaaat
     5161 ctaagcattg gttgcgttgt gtcaaccccg agcgaaaata tttttgagac cagtttcgct
     5221 ggtcggctaa cagcctctgc tagattaacg gcgctgcttg cgagactctt cctcgaaata
     5281 ttttcggtaa agaatgtccc attcacggct tcctgggggc acatgacggg aaagcgagct
     5341 aatcttatgg cgaacgatgt cgtcgatctg atcctcgcgc cggaaaaaat cgttcagagc
     5401 ttctttggtt tccagaagtg ctcgccgctc gttcggaaat tcggcaagcc ttggctggct
     5461 caaaccgctc atgatcaggt gagcaagatg cgagattcgg ccctccgaca atctcataat
     5521 ataa
//
LOCUS       DASULZ010000588         2105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_169470, whole genome shotgun sequence.
ACCESSION   DASULZ010000588 DASULZ010000000
VERSION     DASULZ010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169470"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /locus_tag="VFY96_12160"
     CDS             complement(<1..1383)
                     /locus_tag="VFY96_12160"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX5607265.1"
                     /translation="MKLLFVTPPMGNWAPWGERHLAVNPLYAQVAAFIREKKAADVAV
                     LDCRALGLDDEQMIDAIEERRPDVVFFGAMIAAAGGAAQLNRFHAAMKLIKQSRPETM
                     TVGGGLMYTAVPQKIMAENPQLDFAIVGVFGDNEYPLWELLEEVQKPVPDFAAIRGLA
                     WRKRDEIVLNPARPVISDLDELPMPAYDLFPMNKYYGYSVIPNYNEAVTSRGCEGACH
                     FCYEWWLVDPRNPRDFSSHRTRGGRAVADEMELLNKNYGVKALTFMDDDFNSDRQKMV
                     DLVEALEEKRLDMSWFCLSRAQNLIRDADLIPRLRKVGLYQVLIGIDGGTDEEIAEAR
                     KGNMKVGVKELRDLIQFLRKNDISTISTYLNGFWEDDEAKVRQRAKAVDEIDPDIVMI
                     QLLNPIPGSPVYKKAVKENLIEIQNLSLYDLEHCVMPTKYLSRQQLGELTGWAFQSFY
                     SKPGRIDRILN"
     gene            1610..>2105
                     /locus_tag="VFY96_12165"
     CDS             1610..>2105
                     /locus_tag="VFY96_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607266.1"
                     /translation="MAPVAQEEQLLDIRVAEASAAYKFYLNQERKILGAVSVFIFLSI
                     WELCGNTLQLINPMFMSAPSLIVKAGWQMFASGEIWNDLYVSGIEFFWGYILSIVVAI
                     PFGIAIGWYKKFAYVCDPFVNAMNATPRVALLPLVIIWLGIGIMSKIGIIFLGAVFPL
                     LINTR"
BASE COUNT          450 a          574 c          550 g          531 t
ORIGIN      
        1 gttcaaaatt cgatcgatcc ggccaggctt gctatagaag gattggaaag cccagcccgt
       61 gagctcaccg agttgttggc ggctcagata tttagtcggc atgacgcagt gttcaaggtc
      121 gtagagactc agattttgaa tctcgatcag attttccttt actgccttct tatagactgg
      181 cgagccggga attgggttga gtagttgaat catcacgatg tccggatcga tctcgtccac
      241 ggctttggcg cgctggcgaa ccttcgcttc atcatcctcc cagaagccgt ttagataagt
      301 cgagatggtt gaaatgtcgt ttttgcgcag aaattggatc aggtctcgaa gttctttgac
      361 gccgactttc atgttccctt tgcgcgcttc ggcgatttcc tcgtcggtgc cgccgtcgat
      421 gccgatcaga acctgataga gcccgacttt gcgcaagcgc gggatcaggt ccgcatcgcg
      481 gattagattc tgcgcgcggc tcaggcagaa ccagctcatg tcgagtcttt tttcttccaa
      541 cgcctcgacc aaatccacca ttttttgccg atcggagttg aagtcatcat ccatgaaggt
      601 caaggctttg acaccgtaat ttttgttgag cagctccatc tcgtccgcaa cggcccggcc
      661 gccgcgcgta cgatgcgagc tgaaatcgcg cgggttgcgc ggatcgacga gccaccattc
      721 ataacaaaaa tggcaagcgc cttcgcagcc gcgcgaggtc accgcctcgt tgtaattggg
      781 aatcaccgaa taaccataat acttgttcat cggaaacaga tcgtaggcgg gcatcggcag
      841 ctcgtcgaga tcgctgatca ctggccgcgc cggattgagc acgatctcgt cccgtttgcg
      901 ccaagcgagg ccccggatgg cggcaaaatc cggcaccggt ttttggacct cttcgaggag
      961 ctcccagagc ggatactcgt tatctccaaa caccccgacg atagcgaagt cgagttgcgg
     1021 gttctcggcc atgatctttt gcggcactgc cgtgtacatc aagccgccgc cgacggtcat
     1081 cgtttccggg cgggattgct tgatcaactt cattgccgcg tgaaaccggt tgagctgtgc
     1141 tgctccaccg gccgccgcaa tcatggcccc gaagaaaacg acgtctggtc ggcgctcttc
     1201 gatcgcgtcg atcatttgtt cgtcgtcgag cccgagcgcc cggcagtcga ggaccgccac
     1261 atccgcggct tttttctcgc gaataaaggc cgcgacttgc gcatatagtg ggttcacagc
     1321 caaatggcgc tcaccccacg gtgcccaatt gcccatcggg ggtgtcacaa acaggagttt
     1381 catgagatct cctagtaccg gagagattat tgcgaacaaa aatagttaat acattaatga
     1441 ttgtcaagtt taggcccgct aacgcgcagc aaaaaaacta ttttgactcc ctcgaaaagt
     1501 ttctgcaaag cctacttgaa atgtgttgac aaccctgtag ccttttggta gtggttcgca
     1561 ggctggcaac cttaacgatc gtttaaccgg tttcacagga gggtcattta tggctccagt
     1621 tgctcaagaa gaacaattac tggatatccg tgtcgcggaa gcatccgccg cgtataagtt
     1681 ctatctcaat caagagcgga agattctcgg tgcagtctct gtattcatat tcttgtcgat
     1741 ttgggaactc tgcggcaaca ctttacagct gatcaacccg atgttcatga gcgcgccttc
     1801 actgattgtg aaagccggct ggcaaatgtt cgcatcggga gagatctgga acgatctgta
     1861 tgtcagcggc atcgagttct tctggggata tatcctctcg atcgtggtcg ccattccctt
     1921 cggcatcgcc atcggctggt ataaaaaatt cgcctatgtc tgcgacccgt tcgtgaacgc
     1981 aatgaacgcc acacctcgcg tcgctttgct gcccctggtc atcatttggc tcggtatcgg
     2041 cattatgtcg aagatcggga ttatttttct cggcgccgtg ttcccgcttt tgatcaatac
     2101 ccgcg
//
LOCUS       DASULZ010000589         2429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1184656, whole genome shotgun sequence.
ACCESSION   DASULZ010000589 DASULZ010000000
VERSION     DASULZ010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184656"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /gene="lon"
                     /locus_tag="VFY96_12170"
     CDS             <1..1268
                     /gene="lon"
                     /locus_tag="VFY96_12170"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX5607267.1"
                     /translation="RAMNRKFVRQSLGGVHDEADIRGHRRTYIGSMPGNIIQGIRKAG
                     TRNPVFMLDEIDKLSASFHGDPSAALLEVLDPAQNATFQDHYLGLPFDLTQVLFIATA
                     NVLDTIPGPLRDRMEILELPGYIEEEKLAIAKGYLVPRQMSENGVKPEHIEFTDEGIR
                     EIIRAYTREAGVRQLERQLGSVCRSVATRVAEGQTEQVIISPETVPNYLGPQKFFNDI
                     ALRTSLPGVATGLAWTPFGGDILFVEATKMPGDGRLILTGQLGEVMKESAQAALSLVK
                     SRAEPLGIDPEIFKKNDIHIHIPAGAIPKDGPSAGVTLFVALVSLLTARHISKDVAMT
                     GEISLRGLVLPVGGIKEKTLAAKRAGISCVLLPELNRKDLEEIPASARQDIRFEFLKT
                     VDEALRLALEISAPGGYSPGERFAASPLP"
     gene            complement(1557..2303)
                     /locus_tag="VFY96_12175"
     CDS             complement(1557..2303)
                     /locus_tag="VFY96_12175"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5607268.1"
                     /translation="MRFKDKSIIITGGGGKIAKAYAMAFAKEGAKLSLPDIVSADHTV
                     NAIRDAGGTAITMSCDVSDEQSVKAMVAETVQQFGTVDILINNAAYFMTVWKGPFWEM
                     TVEEFDKAMAVNVRGSWLCAKAVVPHMQKQQRGKIINISSNVALTGNPNYIHYVTSKG
                     ALIAMTRAMARELGDWNICVNTVSPGFVVTEGRKVDPEYEKIRAQQRSIKRTQVENDL
                     VGTVLFLSSPESDFMTGQLLNVDGGFHFVG"
BASE COUNT          518 a          687 c          667 g          557 t
ORIGIN      
        1 cccgcgccat gaaccgcaag tttgtgcgcc agagtctcgg cggagttcac gacgaagctg
       61 atattcgcgg ccatcgccga acttatattg gttcgatgcc ggggaacatt attcagggga
      121 ttcgaaaagc cggtacgcga aatccagttt tcatgctcga cgaaatcgac aaattgagcg
      181 cgagctttca tggcgaccca tcggcggctt tgctggaagt gctcgacccg gcgcaaaacg
      241 cgacttttca agatcattac ttgggtttgc cgtttgatct cacccaagtt ttgttcattg
      301 ccacggcaaa tgtgctcgac accattccgg gaccgctgcg cgatcgcatg gaaatcctcg
      361 agctgcccgg ctacatcgaa gaagaaaagc tggccatcgc caaaggctat ctcgtgccgc
      421 gccagatgag tgagaatggc gtcaagcccg agcacatcga atttaccgat gagggcatcc
      481 gagagattat tcgtgcctac acgcgcgaag ctggagtgcg ccagctcgag cgccagttgg
      541 gaagcgtgtg ccgaagcgtc gcgacgcgcg ttgccgaggg acaaaccgaa caggtcatca
      601 tcagtccgga aaccgtaccg aactatttgg gtccgcaaaa gtttttcaat gatattgcgc
      661 tgcgcaccag tttgcccggc gtcgccaccg gcttggcctg gacaccgttc ggcggcgata
      721 ttctattcgt cgaagcgacc aagatgccgg gtgacggcag attgattttg accggacagc
      781 tgggcgaggt aatgaaagaa agcgcgcagg ctgccttgag cctggtgaaa tctcgcgcgg
      841 aaccgctcgg catcgatccc gaaatcttca agaagaacga tattcatatt catatcccgg
      901 cgggtgcgat tccgaaggat ggacccagcg ccggcgtgac gctctttgtc gcgctggttt
      961 cactgctgac cgcccgtcac atcagcaagg acgtcgctat gaccggggag atcagcctgc
     1021 gcgggctggt gcttccggtg ggcggcataa aagaaaaaac gctggctgcc aagagggccg
     1081 gcatttcctg cgtgctcttg ccggaactca accgcaagga tctcgaagag attcctgcca
     1141 gtgcccgcca ggatatccga ttcgaatttt taaaaaccgt ggacgaggcg ttgcggcttg
     1201 ctttggagat cagcgcgccc ggcggttatt ctccgggaga aagattcgcg gccagcccgt
     1261 tgccttgagc tagaaattta ccaacatggg ttttccctga gcttacgctc gctgctagaa
     1321 gatgaattgg cgcagcgcag ccgcaggcac cctctaacgt tctaatcggg gagtcagtcc
     1381 gaattccgcc tggattgcgg ctaaaacatg cggcaatggc gtatccgctt cacatttaaa
     1441 caagcgacaa aggaacacgc cgcggtggtg ttgtcatttc gagcacagcg aaaaatcttg
     1501 cattctccgg gcgtttcgtg ttctccgtgc ctctgcggtg aatattctct cctcccctat
     1561 ccgacaaagt gaaaaccgcc gtcgacgttc aacagttgcc cggtcatgaa atcgctttcc
     1621 ggcgaggata aaaacagcac cgtgccgacc agatcatttt ctacttgcgt gcgcttgatg
     1681 ctgcgttgct gcgcgcggat tttctcatac tcgggatcca ctttgcggcc ttcggtcacg
     1741 acgaaaccgg gactcacggt attcacgcag atattccagt cgccgagctc tcgcgccatc
     1801 gcgcgcgtca tcgcgattaa tgcgcccttc gaagtcacgt agtggatgta attggggttg
     1861 ccggtcaagg ccacgttcga agatatattg atgatcttgc cgcgctgctg cttttgcatg
     1921 tgcggcacca ccgctttggc gcacagccag gaaccgcgga cattgacggc catcgctttg
     1981 tcgaactcct ccacggtcat ttcccagaac ggtcccttcc agaccgtcat gaagtaggca
     2041 gcgttgttga tcagaatatc caccgtgccg aattgctgca cggtttcggc gaccatagct
     2101 ttcacgctct gttcatccga tacatcgcaa ctcatggtga tcgcggtgcc tcctgcatcg
     2161 cggatggcat ttaccgtgtg atcggcgctg acgatgtccg gcagcgagag cttcgcgcct
     2221 tccttcgcga acgccatcgc ataggctttg gcaatttttc caccgccgcc ggtgatgatt
     2281 atggatttgt ctttgaaacg cataactctc ctcctaaccg agttcgtgtt ctgctccgaa
     2341 tgtcgctatt attgtcacac aatgtgcgtc gaaaaaatct tccctgccct gtctttgaca
     2401 agagggtgag cgcgtccccc tttgaaagg
//
LOCUS       DASULZ010000590         2405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_474151, whole genome shotgun sequence.
ACCESSION   DASULZ010000590 DASULZ010000000
VERSION     DASULZ010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474151"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /gene="tcuA"
                     /locus_tag="VFY96_12180"
     CDS             <1..1287
                     /gene="tcuA"
                     /locus_tag="VFY96_12180"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEX5607269.1"
                     /translation="DEIRQFLPDIDDATFAKLHLPAYTAEQFHADLQRVTRGRISKEL
                     SEVLVSQSNSAIHWMRDVGIKWDIDSHVIIDGRYYFEPGLVIHPVGGVSGGLGQLMQW
                     REIATRMGIELRYESRVTALLGNDRSIQGVRVSDRHEEYEIRTNSLILCAGGFQASAE
                     KRARYLGPNADLMKVRGSKHDTGEVLMMAIELGAKTAGHWQGAHATPIDATFPDVEIS
                     NKANRYGYTYGITVNSLGERFFDEGEARHSYTYAKTGWAVLGQPGAIAYQIYDQKAAR
                     LLNQRYYEFSHPIEADRIDELATKIGIEPAVLNHTIAEFNRAVRKDIPFDATKLDGRS
                     TEGITPKKSNWADTIDKPPYWAYAVTGGITFTFGGLQINTQAQVLNTSNNPIRGLFAS
                     GDIVGLFFHNYPSCTGQTRNVVFSRLAGRNAAAQGL"
     gene            complement(1387..1719)
                     /locus_tag="VFY96_12185"
     CDS             complement(1387..1719)
                     /locus_tag="VFY96_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin-B12 independent methionine synthase"
                     /protein_id="HEX5607270.1"
                     /translation="MSRKLFRNLKAQRLLLEYDDARSGTFEPLRDVAEDKFVVLGLVT
                     TKRPELEALDLLTERITEASRYVSMERLGLSPQCGFASSIIGNRISFEDQRSKLELVV
                     KTARAVWG"
     gene            complement(1768..>2405)
                     /locus_tag="VFY96_12190"
     CDS             complement(1768..>2405)
                     /locus_tag="VFY96_12190"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX5607271.1"
                     /translation="ITAAQFKEIEDHAVDEAIALQEKIGFEIVSDGEMRRQSFQSQMT
                     AAVSGFGEFDLDAFLWGDWHDEQGVERAPRPRRLGVVATLNRLRYLSAHEFSYLKSKT
                     HRIPKITLPSPGLWANFWSPKYSRAAYPTLEDFLADITAILREEITELVRLGARYIQI
                     DAPHYGLLLDSKTRAFYEKLGWNLERWLSLGVELDNTVMQGFSDVTFGFHI"
BASE COUNT          546 a          682 c          667 g          510 t
ORIGIN      
        1 gacgagattc gccagttcct gcccgatatc gacgacgcga cgttcgccaa gcttcatctg
       61 cccgcttaca ctgcggagca attccatgct gacttgcaac gggttacgcg cggccgaatc
      121 agcaaagagt tgtccgaagt cttagtgtcg caatcgaact cggcgattca ctggatgcgc
      181 gacgtcggca tcaagtggga catcgacagc catgtgatca tcgacggccg ttattacttc
      241 gagccgggtt tggtgattca tcccgtaggt ggtgtgagcg gcgggctcgg acagctcatg
      301 caatggcgtg agatcgcgac ccgcatggga atcgagctcc gctacgaatc ccgcgtcacc
      361 gcgctgctcg gcaacgaccg cagtattcaa ggcgtgcgcg tctccgatcg gcacgaggaa
      421 tatgaaattc gcacgaattc acttattctc tgtgccgggg gttttcaggc gagcgcggaa
      481 aaacgcgcac gctatctcgg tcctaatgcc gatttgatga aggtgcgcgg cagcaagcat
      541 gataccggcg aggtgctgat gatggccatt gagctcggcg caaagacagc cggccattgg
      601 cagggcgccc atgcgacgcc gattgatgcc acattcccgg atgtcgaaat cagcaacaaa
      661 gcgaatcgct acggttacac ctacggtatc acggtcaact ccctgggcga gcgttttttc
      721 gatgaaggcg aggcgcgcca ttcctatacc tacgccaaaa ccggttgggc agtgctcggt
      781 caaccgggcg ccattgcgta tcagatctat gatcagaagg cggcgagact attgaaccaa
      841 cggtattacg agttttctca tcccatcgaa gccgacagga tcgacgagct ggcgacaaag
      901 atcggcatcg agcccgccgt gttgaatcat acgattgctg aattcaaccg tgccgtgcgc
      961 aaagatattc cgttcgatgc caccaagttg gatgggcgct caaccgaagg cattacaccg
     1021 aagaaatcca attgggccga caccatcgac aagccgccct attgggcata cgcggttacc
     1081 ggcgggatta cattcacgtt cggcggctta cagatcaata cccaagccca ggtgctcaac
     1141 acgtcgaata atccgatccg gggcttgttc gcctccggcg atatcgtcgg gttgttcttt
     1201 cataattatc cttcgtgcac gggccaaaca cgcaacgtgg tcttttcgcg cctggcggga
     1261 agaaacgcag ccgcccaggg gctgtagacg gaagcgtgcc aaaatcgtca ttgcggtcaa
     1321 cgcagtttgg cgttacgcga gccgaaatcc aaagcctagg gccgtatggc atacactctc
     1381 tgtgctttat ccccacaccg cgcgcgcggt tttcacgaca agttctagtt tactgcgttg
     1441 gtcttcgaaa gaaattcggt tgccgatgat cgatgatgca aagccgcact gcgggctcaa
     1501 gcccaatcgt tccatcgaga cgtaccggct ggcctcggta atgcgctcgg tcagaagatc
     1561 cagtgcttca agctccggcc gcttagttgt gactaatccg agaacgacaa atttatcctc
     1621 cgccacatcg cggagcggct caaaagtgcc cgagcgggca tcgtcgtatt cgaggagcaa
     1681 gcgttgcgcc ttgagatttc gaaaaagctt gcggctgatc gcttcataac ttccttggga
     1741 aagccaccgg ctcccttgat tgccgcgtca gatatgaaag ccgaaagtta catcggaaaa
     1801 cccctgcatc acggtattgt cgagctccac gccgagagag agccagcgct ccaaattcca
     1861 gccgagcttc tcgtaaaaag cacgcgtttt ggaatcgagc agtagaccat aatgcggcgc
     1921 atcaatctga atgtagcgcg ccccgaggcg gaccagttcc gtgatttcct cgcgcaagat
     1981 ggcagtgata tcggcgagaa aatcctcgag ggtcggatag gcagcgcggg aatacttcgg
     2041 cgaccagaaa tttgcccaca gcccggggct gggaagcgtg atcttcggaa tccggtgggt
     2101 tttacttttc aggtacgaaa actcgtgggc cgagagataa cgaagccgat tcagtgttgc
     2161 gactacgcct agccggcgtg gacgcggtgc tctctcaacg ccctgttcat cgtgccagtc
     2221 accccaaaga aatgcgtcga gatcaaattc gccaaagccg ctgaccgccg ctgtcatttg
     2281 gctctgaaag gactgccggc gcatctcgcc gtcactcacg atctcaaagc cgatcttctc
     2341 ctgcagtgcg atggcctcat cgaccgcatg atcttcgatc tctttgaatt gagccgcagt
     2401 gattt
//
LOCUS       DASULZ010000591         4023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_371593, whole genome shotgun sequence.
ACCESSION   DASULZ010000591 DASULZ010000000
VERSION     DASULZ010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371593"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(287..1327)
                     /locus_tag="VFY96_12195"
     CDS             complement(287..1327)
                     /locus_tag="VFY96_12195"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607272.1"
                     /translation="MQRGQQLEGKTRREKRMTGRVVRSICLLLAVFLPAVSAKAQQAG
                     KIYRVGRLSGGLSSSRFSLDALRRELRDLGYLEGNNISFELRSAEEKSERLSALAHEL
                     VRLKVDLIIAGGPNDGLTAKKATKTIPIVFTDSPSDPVAYGLVDSMARPGGNVTGFYS
                     MADVLAGKRLQILKESIPKLSRVAVLWYGRSGGSESQWAESQRTARQLGLQLHSMEIS
                     NTGNYESAFKEAIKTGSTALAVVRHRQSQTPVNQERIIKLAAKYDLPAIYYREDFVEQ
                     GGLMSYGADELEPFKRVAAIIDKILKGMKPAAIPVEQSSKFEFVINLKTASQIGLTIP
                     PNVLARADRVIR"
     gene            complement(1628..2599)
                     /locus_tag="VFY96_12200"
     CDS             complement(1628..2599)
                     /locus_tag="VFY96_12200"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607273.1"
                     /translation="MIVIALSIWGAVASAQLSKLTIGNNTLSAAGLPAWMAKESGIFQ
                     KNGLEVQIVYFRGGTITTMSLVARETPISQVSGPPMVSAALKGADAVMIAGGNVIAEY
                     WLMSKAEIKTAEQLKGGTVAIATFGGLSDGMARIALQKLGLNPVKDVTLIQIGTIPER
                     LSALDSGKVQGAMLNAPDNFRAQKRGLHKLMGVRLPYQGIGVGTTRAVIRDNPDVVRR
                     YIRSQVEAVHRLKTDREAGMRVLGKYLALQDKEILQKAYDDGVDDEILPAKQYPTLEG
                     IKKILEPLGETDPKAKAAKPEDFVDMRFIRELDESGFTNDLYRGEKK"
     gene            complement(2737..3180)
                     /locus_tag="VFY96_12205"
     CDS             complement(2737..3180)
                     /locus_tag="VFY96_12205"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEX5607274.1"
                     /translation="MAFRESISAAFGVQIQYVELKVPKDIDPAFGAARKAAADAVVVL
                     TSVVTNTNRKPIIELAAKNRLPAMYYTAEWAQNGGLLSYGANFPDLFRRAATYVDKIL
                     KGTKPGDLPVEQARKFELIVNLKAAKEIGLSIPVNVLARADRVIR"
     gene            complement(3214..>4023)
                     /locus_tag="VFY96_12210"
     CDS             complement(3214..>4023)
                     /locus_tag="VFY96_12210"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607275.1"
                     /translation="YIEGKNIVIEWREAQGNFDRIRELADELVRLKMDVIVSPGPAVT
                     RPLKEATSTIPIVMAQDTDPVGSGFVATLAHPGGNITGLAALAPEMAGKQLELLKEII
                     PRLSRVAIIGNSNNPGDAQALRETVIAAGTVEVYLRYLDVLDPKDIESKFGAAAKGRA
                     DAVLMLGNPLLNSRRKQIVELAVKHRLPTAYTRPEYIDEGGLMYYGTNYNDLFRRAAG
                     YVDRILKGAKPADLPVEQPTKFELILNLKAAKQIGRTIPNKVLAKADRVIR"
BASE COUNT          850 a         1117 c         1042 g         1014 t
ORIGIN      
        1 tccgagaccg cgttcattcg ctgttcgggt gaagcgttgt cgaaaccttc cagttcgggc
       61 ctgcgatcgc cgggccgaat aacgcgagtg gtgatcgttt tccgtttagt gcgaagatcc
      121 atgtaggtct tttatagcac atccaaaaaa agacttctcc cattgcaact gaggaacgac
      181 ttgagccaaa caaagccggt gatacccgcc gattacttgc tggactgagc gatgaaacag
      241 ccggaggcgt agctttatct cctcgtgacg cctgaggctt catccgtcac ctgatcaccc
      301 gatctgcccg cgccagcaca ttcggcggaa ttgtgaggcc gatttgcgag gcagtcttga
      361 gattgattac gaactcgaat ttgcttgatt gctcgaccgg aatggcggca ggcttcattc
      421 cttttaggat tttatcgatg atcgccgcaa cccgcttgaa cggttcgagt tcatcggcgc
      481 cgtaggacat caagccgcct tgctcgacaa aatcttcgcg ataataaatt gccggcaaat
      541 catactttgc cgcgagctta atgatccgtt cttgatttac gggggtttga gactgacggt
      601 gccgcacaac ggctagagcg gtgctgcccg tcttgatcgc ttctttgaac gcgctctcat
      661 agttacctgt gttgctgatc tccatggagt gaagctgcag cccaagttgg cgcgcggtgc
      721 gctggctttc tgcccattgt gactcgctgc ctcctgacct tccgtaccac aagacagcga
      781 cacgcgagag cttaggtatc gattctttga gaatctgcaa tcgcttgcct gccaacacat
      841 ccgccatgga gtaaaatcca gtgacgttgc caccgggccg cgccatgcta tcgaccaacc
      901 catacgcaac cggatcggaa ggggaatccg tgaaaacgat gggaattgtt ttggtcgcct
      961 tcttggcggt caggccatcg tttggaccac ccgctatgat gagatcgacc ttgaggcgaa
     1021 ccagctcatg agctaaagca gagagccgct cggatttctc ttcggcggag cgaagctcga
     1081 aagagatgtt attgccctca agataaccca gatcgcgtag ctctcgtcgt agtgcgtcga
     1141 ggctgaatct ggaactagat aagcccccag ataatcttcc aacgcgataa attttcccgg
     1201 cctgctgcgc cttcgctgaa acagcaggca aaaagacagc cagcagtagg cagatagagc
     1261 ggacgactcg tccggtcatg cgtttctctc gccgcgtttt gccttccaac tgctgacctc
     1321 gttgcactat caacgtataa ctccgtcggc tcgcgccagg acattaggcg gaatcgttaa
     1381 gccgatctgc ttggccgttt tgagactgat cataagctcg agctttgtcg gctgttgacg
     1441 ggcaagtcgg cgggcttggc gcttttcaac gtcaatcgct gctcgcggga ataaatcctc
     1501 taagtttgtg acataggaaa tgatcaccgg cgcctgccgg aaatgatcgg cacccgtgca
     1561 tgttgctggt tcatcccttc tcttagctct tgctgttact ccttgcccgc tatctgctat
     1621 ctcgctgcta cttcttctcg cccctgtaca aatcattggt aaagccactt tcgtctaatt
     1681 ccctgatgaa acgcatgtcg acgaaatcct caggcttcgc cgctttggcc tttggatccg
     1741 tctctcccaa gggttcgaga atttttttaa tcccttccag agttggatac tgttttgcgg
     1801 gaagtatctc gtcatcaacc ccatcgtcat aagctttttg taaaatttct ttgtcctgta
     1861 aagcgaggta cttccctaac actctcatgc cggcttcgcg atccgtcttc agacggtgca
     1921 ccgcttcgac ttgagatcgg atataccggc gcaccacgtc gggattgtcg cgaatgaccg
     1981 cgcgtgtcgt acccacgcca attccttgat acggcagacg cacacccatt aatttgtgga
     2041 gaccgcgttt ctgtgccctg aagttatcgg gtgcattgag catagcaccc tgaacttttc
     2101 ccgaatcgag ggcgcttagg cgctccggga tcgtgccaat ttggatgaga gtaacgtctt
     2161 tcaccggatt taatccgagc ttctgcaggg caatacgagc catcccgtcc gacaagcccc
     2221 caaaggtagc gatcgcgacc gtgccgcctt tgagctgctc ggcggttttg atctcggcct
     2281 tgctcatgag ccagtactct gcgatcacgt tgcctcccgc gatcattacc gcatcagccc
     2341 ctttcagagc ggcacttacc atcggcggac cggagacctg actgatgggc gtctcccgag
     2401 caactaagga catggtggta atcgtgcctc ctctaaaata gacgatctgt acctcgaggc
     2461 catttttctg aaatatcccg gactctttag ccatccatgc gggaagacca gcggcgctaa
     2521 gagtgttatt accgatcgtc aacttgctga gctgcgccga tgccacagct ccccatatcg
     2581 agagtgcaat gacgatcatg agaagcaaca cccattttcg tttttggatc cttcgacttg
     2641 gcgcagaaca ggttgtagtt ttttgattcg cagagtgagg gctcaacaag tgtatccaag
     2701 atttagtgtt catgtgttca attcctagtc taaatatcac ctaatcactc gatccgctct
     2761 ggcgagcaca ttcacgggaa tcgacaagcc gatttctttg gcagccttga gattcacgat
     2821 gagctcgaat ttgcgcgcct gttcgaccgg gagatcaccg ggctttgtgc ctttcagaat
     2881 cttgtcgaca tacgtcgccg cgcgccggaa tagatcggga aagtttgcgc catagctaag
     2941 aagtcctcca ttctgagccc attcagcggt gtaatacatt gccggcagcc gatttttcgc
     3001 cgcaagctcg atgatcggtt ttcgattggt attcgtgaca acgctcgtca gcacgacgac
     3061 cgcatcagcg gccgccttgc gcgccgcacc aaacgcggga tcgatatcct tcggaacttt
     3121 taattcaaca tactggatct gcactccgaa ggctgctgag atggattctc gaaaagccaa
     3181 aacccgtcct gaaggaatgt gaagggtcgt gcattaccga atcacccgat ccgctttcgc
     3241 taatactttg ttcgggattg tgcggccgat ctgcttggcg gctttgaggt tcaagataag
     3301 ctcaaacttg gtgggttgct caaccggtag atcggcgggc ttggcaccct tcaagattct
     3361 atcgacatag cctgccgcgc ggcggaacaa gtcgttatag ttcgtgccgt aatacatcag
     3421 cccgccttcg tcgatgtatt ccggcctggt ataggcggtt ggcagcctgt gtttgaccgc
     3481 gagctccacg atctgtttgc ggcgcgaatt gagtaagggg ttgccgagca tcagtactgc
     3541 atcggctcgt cctttagcgg cggcgccaaa tttcgactcg atgtccttgg gatcgagcac
     3601 gtcgagatag cgaagataga cttcgaccgt gccggcggcg atcaccgttt ccctgagcgc
     3661 ctgtgcgtct cctggattgt ttgaattccc aataatcgcg acacgtgaaa gcctgggaat
     3721 gatttccttt aaaagctcga gttgcttgcc tgccatttcc ggagccagag ccgccaatcc
     3781 ggtgatattg ccacccgggt gcgccagggt ggcgacaaac ccgctgccca ccggatcggt
     3841 atcctgtgcc atgacaatgg gaatcgtcga agtggcttcc ttgaggggtc gggttaccgc
     3901 cgggccaggc gagacgatga cgtccatttt gagacgcacc agttcatccg ccaattcacg
     3961 gatgcgatca aagtttcctt gggcctcgcg ccactcgata acgatgtttt ttccttcgat
     4021 gta
//
LOCUS       DASULZ010000592         4113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_576118, whole genome shotgun sequence.
ACCESSION   DASULZ010000592 DASULZ010000000
VERSION     DASULZ010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576118"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VFY96_12215"
     CDS             complement(<1..560)
                     /locus_tag="VFY96_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5607276.1"
                     /translation="MAAVDGARPPSFAAIAKKTMPAVVNIATALQRSSRASNDPIEEF
                     FSRFFGESTSRENNLRSLGSGILINKDGEILTNYHVVRYADTIKVKLADQSEHEARLV
                     GKDERTDLALIKIRKSNGNLPFAKLGASVPLEVGDWVMAIGNPFGLEHTVTAGIVSAK
                     GRVIGAGPYDNFIQTDASINPGNSGGP"
     gene            1001..2356
                     /gene="mgtE"
                     /locus_tag="VFY96_12220"
     CDS             1001..2356
                     /gene="mgtE"
                     /locus_tag="VFY96_12220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX5607277.1"
                     /translation="MKPAPIDYDIVREMLRTATPDRILRFIGKSHPADIALLFKGLQP
                     FEVRQLFDILFSSRRADKTLKELPPELLPDILELIDDEKIGRLIVRADPDDAVTFIDS
                     LPDNRREKILALLDPERQAAVRELINYPEGTVGRIMTTDFMALPPDTTAQGAIDKIRE
                     RGELESFFYLYVIDEAGKLVGVVPIRNLVIAPRDRRLSDMMIAEPVKANVFMDQEEAA
                     RLVSRYELLALPIIDEAGRLEGIITVDDVIDIINEESTEDMYKMAGLAEEDRVFTPIS
                     RSVRKRLPWTILNLVTASLAASVVGFFEGTLHEILALVMFMPVIAGVGGNGATQTATV
                     IIRAIALGELEFASAWKAIVKQVSVNICIALAAGALIALAAILWKGNPFLGAVLATAM
                     IFNLGLMAGFAGAVIPLLLKALKFDPALGAGIIVTGLTDAFGFLSFLGLATFFRQYLV
                     N"
     gene            complement(2349..3329)
                     /locus_tag="VFY96_12225"
     CDS             complement(2349..3329)
                     /locus_tag="VFY96_12225"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX5607278.1"
                     /translation="MKKAKPILILLSKLLVSVGLLGYFLARIHIERFLQTFASAKFSY
                     IAIALLVYLATQAVSAMRWTALARPLGVKTPFKDMTRYYLIGMFFNLFAPGTVGGDVS
                     RVYYLVRDENARLKDRSVTTMHAAMSVLMDRAIGMVVLVWLGAAGLLLFPYYAVPHSA
                     RVATFLLSLGLLAGALITPLVRRLLPTDSHQLIVKVRLAFSAYRKHWRALVTAALFSL
                     AIHLVQSWMHVVMGRALDLNVPFSFALIVYPLVGTFSAIPISLNGLGLREGGYVVLLS
                     VIGIGTEKAIAFGILLFLIVACDSLIGGILYLFQQGAKPDEAALENARAS"
     gene            complement(3326..3988)
                     /gene="purN"
                     /locus_tag="VFY96_12230"
     CDS             complement(3326..3988)
                     /gene="purN"
                     /locus_tag="VFY96_12230"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX5607279.1"
                     /translation="MAEKLPIAVLISGNGTNLQSIIDAIAANRLDAKIEIVLSNRADA
                     FGLVRAKKHGIPNGVLDHKSFPSREAYDQAIVDLLRARGVQLVALAGFMRLLSPVLIT
                     AFSNRIMNIHPALLPSFPGLHVQKKALEHGVRFSGCTVHFVNEECDEGPIIIQAVVPV
                     FTDDTEDELAQRILKQEHRIYPRAIQLYAEGRLHVVGRKVLVDGLSKNDSQSLIHPPL
                     DG"
BASE COUNT          904 a         1155 c         1145 g          909 t
ORIGIN      
        1 ggaccgccgc tgtttcccgg attgatcgag gcgtcagtct gaatgaagtt gtcgtacggc
       61 ccggcgccga tcacccgtcc cttggcgctc acgatgcccg ccgtaacggt gtgttcgagg
      121 ccgaacggat taccgatcgc catgacccag tccccgactt cgagcgggac agacgcgccg
      181 agttttgcga acggcagatt accgttcgat tttctgatct tgatcagggc caaatcggtg
      241 cgctcgtctt tgccgacgag gcgcgcctcg tgctcgctct gatcggcgag tttgactttg
      301 atcgtgtctg cgtagcggac cacatgatag ttggtcaaaa tttcgccgtc cttgttgatc
      361 aggatacctg agccgagact ccgaagattg ttctcgcgcg atgtcgattc accaaagaaa
      421 cggctgaaga actcctcgat cggatcattc gaggcacgag aagaacgctg cagcgcagtc
      481 gcaatattga ccaccgccgg catggttttc ttggcgatcg ccgcaaacga tggcggacgg
      541 gcgccgtcga ctgccgccaa actcgccgca gagctcccgt cgctggtaac cgcgagggca
      601 aagcaaagcc aaagtgcatg tttcagggct gatgatagcc ccagccgagt gtctcggaat
      661 atgcaaatcg tgctgtgtct cacctaatat cctgcagcca aagttcgcaa tatttctccc
      721 tccgattcgc caaaatctcc cgttccctcc ttttccaaag aggcgcaaaa aggtggagaa
      781 ttccccgttt gaaaaagggg gatacagggg gattttagtg agaccgcagc cgttgccatc
      841 gttgtgaatg tttaaacaaa ctctggtaca cgaccagaat cctcgcgcca taccctagca
      901 aatttctctc agcgatttaa acctgaccta aattagcgat ccggcaattg caaattgggt
      961 cccttgccgt tatgaacaat cgatcttttg ctttgcgctc atgaaacccg ctcctattga
     1021 ctacgatatc gtccgcgaaa tgctgcgcac cgccacgccg gaccgcatcc taaggtttat
     1081 cgggaaaagt catcccgctg acatcgcgct cttgttcaaa ggactgcagc cgttcgaggt
     1141 gcgacagctc tttgacattc tcttctcctc gcggcgcgcc gataaaacgc tgaaggagct
     1201 gccgccggag cttttgccgg atattctcga attgattgac gacgagaaaa tcgggcggct
     1261 gatcgttcgg gccgaccccg atgatgcggt cacgtttatt gatagtctgc ccgacaatcg
     1321 ccgtgagaaa attctcgcgc tcctcgatcc ggagcgtcag gcagcggtcc gcgagctgat
     1381 caattatccc gaaggcactg tcggcagaat catgaccact gattttatgg cgcttccccc
     1441 cgacaccact gctcaaggcg ccatcgacaa gattcgcgag cgcggcgaac tggaatcatt
     1501 tttttacctt tatgtgatcg atgaagcggg caaactggtg ggagtcgtac cgatccgcaa
     1561 cctcgtgatc gcgccgcgcg accggcggct gtctgacatg atgattgccg agccggtcaa
     1621 agccaatgtg ttcatggatc aggaagaagc cgcccggttg gtctcgcgct acgagcttct
     1681 ggcgctgccg atcatcgatg aggcgggccg cctggaagga attattacgg tcgacgacgt
     1741 gatcgacatc attaacgagg aaagcaccga agacatgtac aagatggcgg gcttggccga
     1801 agaggatcgc gtgttcacgc cgatttcccg ctcggtaaga aaacgtctgc cgtggacgat
     1861 cttgaatctg gtgaccgcca gcctggcggc gtcagtagtc ggcttctttg aagggacctt
     1921 gcacgagatt ctcgccttgg tgatgttcat gcctgtgatt gccggcgtcg gcggtaatgg
     1981 cgcgactcaa accgctaccg ttattatccg cgccatcgct ttgggcgagt tggaattcgc
     2041 ctctgcctgg aaagcgattg tgaagcaggt ttcggtgaac atttgcatcg cgctcgcggc
     2101 cggtgctctg atcgcactgg ccgctatttt gtggaaaggc aatccgtttc tcggcgccgt
     2161 gctcgccacc gccatgatct ttaatctggg actgatggcg ggattcgctg gcgcggtcat
     2221 tccgctcttg ttaaaagcgc tgaaattcga tccggctctc ggcgccggca ttatcgtcac
     2281 agggctcacc gatgcctttg gctttctgtc atttctcggt ctcgcaactt ttttccgtca
     2341 atatctcgtt aactagcgcg ggcgttctcg agcgccgctt cgtcaggctt ggcgccttgt
     2401 tggaaaaggt agagaattcc gccgatcagg ctatcgcagg cgacgattaa aaaaagcaag
     2461 attccgaacg caatcgcctt ctcggtgccg atgccgatga ccgataaaag gacgacgtac
     2521 ccgccctcgc gtaaaccgag gccgtttaga ctgatgggga tcgctgaaaa agttcccacc
     2581 agcggataga caatgagcgc gaaggaaaac ggcacattca aatcgagagc gcggcccatc
     2641 accacatgca tccacgattg caccagatga atcgccagcg agaaaagcgc ggcggtaacg
     2701 agagctcgcc aatgtttgcg gtaggcactg aacgcaagcc gtaccttgac aatcaattga
     2761 tgactgtcgg tgggcagcag ccgccgcacg agcggcgtaa tcagtgcgcc cgcgagcaaa
     2821 cccagcgaga ggagaaacgt cgcgacccgc gccgaatgag gtacggcata atagggaaac
     2881 agcaacaatc cggcggcgcc aagccagacg agcacaacca tgccgatcgc ccgatccata
     2941 agaaccgaca ttgcggcatg catcgtggtc accgaccgat ctttcaggcg tgcattttcg
     3001 tcacgcacga ggtaataaac ccggctcaca tcgccgccca ccgtgcccgg cgcgaacagg
     3061 ttaaagaaca tgccgatcag ataataacgc gtcatgtctt taaacggcgt ctttactccg
     3121 aggggccgcg ccagggcggt ccaccgcatg gcgctgaccg cctgagttgc caggtaaacg
     3181 agaagcgcta ttgcgatata ggaaaatttc gcgctcgcaa aggtttgcag aaagcgttcg
     3241 atatgaatgc gcgcgagaaa atatcccagc aggccgacgc tgaccagcag ttttgaaagc
     3301 agaatcagga tcggcttggc ttttttcatc cgtccagcgg cgggtggata agactttgcg
     3361 agtcattttt gctcagaccg tccaccagca cttttcgacc gacgacatga agccggcctt
     3421 cggcgtagag ttgaatcgcg cgcggataaa ttcggtgttc ctgctttaaa attctttgcg
     3481 ccagttcgtc ctcggtatcg tcggtgaaca ccggaacgac ggcttgaatg atgatggggc
     3541 cttcgtcgca ttcttcattg acgaagtgaa cggtacaccc cgagaaccgc accccgtgct
     3601 cgagcgcttt cttttggacg tgcaatccgg gaaaggatgg caacaatgcc ggatgaatgt
     3661 tcatgatccg gttcgagaaa gcggttatca agaccggtga cagcagccgc ataaatcccg
     3721 cgagcgcaac cagttgcaca ccgcgcgcgc ggagcagatc gacgatggct tgatcgtaag
     3781 cttcgcggct gggaaacgac ttgtgatcga ggactccatt cggaatgcca tgtttttttg
     3841 cgcgcaccag gccaaaggca tcggctcgat tgctcagcac aatttcgatt ttcgcgtcga
     3901 gtcggttggc cgcgatcgca tcgatgatcg actgcaagtt ggtgccgttg ccggagataa
     3961 gcaccgcgat cgggagtttc tctgccatcg gtgtcgtcag gcgcggataa cggcgcctcg
     4021 tgttcctttt tttatttcgc caatgacgta agacttctca cccattttcg tgatcgcctg
     4081 ccgcagtgcc tcagcgcggt ttttgccggc gac
//
LOCUS       DASULZ010000593         2164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1285932, whole genome shotgun sequence.
ACCESSION   DASULZ010000593 DASULZ010000000
VERSION     DASULZ010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285932"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VFY96_12235"
     CDS             <1..323
                     /locus_tag="VFY96_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5607280.1"
                     /translation="RSIAMNAETFSSGQDLLDLLEAMPSFRPDCLILDVQMPRMSGLA
                     VQDHLARCGKTFPVIFITAHDDVGIREKALAAGAVAFLRKPFVDELLIKTLQEALRRG
                     GAPN"
     gene            376..507
                     /locus_tag="VFY96_12240"
     CDS             376..507
                     /locus_tag="VFY96_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607281.1"
                     /translation="MKQVIFLICARLISSIAFGQVYSYPNRGQSQQQQDKDRYECHT"
     gene            511..693
                     /locus_tag="VFY96_12245"
     CDS             511..693
                     /locus_tag="VFY96_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607282.1"
                     /translation="MQQTGFDPTGAQASAPPPSGSQRGGEVVRGAARGALVGTVGVRM
                     RPMPELARLQVPQWVG"
     gene            711..851
                     /locus_tag="VFY96_12250"
     CDS             711..851
                     /locus_tag="VFY96_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607283.1"
                     /translation="MDKAKAQEQQQAQQQNYNTQQQQAGYDRAMGACLEGRRLHGSIG
                     EI"
     gene            1016..>2164
                     /locus_tag="VFY96_12255"
     CDS             1016..>2164
                     /locus_tag="VFY96_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607284.1"
                     /translation="MRHSSTGKMRTLVLLLLSLTLLHDPIPVSAQTSFEPPATLSASK
                     ILPPDLLSGPNHRVEERVYNDGYLNTYRVGSKFGTFVAVSTPMLRKRIGEINALVRME
                     QIQGTKEFTSALKEAGTDTLVGFKNLVTSPVETVKGAASGLGVAFRRASDALTGPKRS
                     DSEDSKLKDLIGFSKTKREYAYQLGVDAYTDNEKLQDRLDEISWAGYAGGITWAGAMA
                     AVPGGAGLAITISGTHKLLNEVFRTTPPVDLRRMNGEKLKAMDVHPEVADAFLNNSVY
                     SPRDQTLLVNALEEMKGVGNRATFVRLAASSGDRNIAFFRERQAEMYAGFHKAVAAVE
                     TFIALGEFAAVRTTKNDVVFNVPLDHLVWTQDMAKLLTAADARVTQLTR"
BASE COUNT          515 a          555 c          623 g          471 t
ORIGIN      
        1 tccggtccat tgccatgaat gcagaaacat ttagctcggg gcaagatctt ctcgatcttc
       61 tcgaggcgat gccttctttt cggcccgact gcctgattct ggatgtgcaa atgccgagaa
      121 tgagcggcct cgccgttcaa gatcatcttg cccgatgcgg caaaacgttt cctgtcatct
      181 ttataacggc tcacgatgat gtcggaatca gggagaaggc actggccgct ggcgcggtgg
      241 cgtttttacg taaacctttc gttgacgagt tgctgattaa aacgctccaa gaagctctga
      301 gacgcggagg cgctcccaat tagtcggttc acaccgcgat catcattgat aggggaaata
      361 aatgctggag gtccgatgaa acaggttatt ttcttgattt gcgcaaggct tatttcgagc
      421 atcgctttcg ggcaagtcta cagctatccg aataggggac aaagccagca gcagcaagac
      481 aaggatcgct acgagtgtca tacctgagca gtgcagcaga ccggctttga tcctacgggt
      541 gctcaggcat cagcgccgcc gccgagcggt tcccagcgcg gtggtgaagt ggttcgcggt
      601 gcagcgcgag gcgctctcgt cggcacagtt ggcgttcgaa tgcggccgat gccggaactg
      661 gcgcggctgc aggtgccgca atgggtgggt tagtgggcgg aatgcgaaga atggacaaag
      721 cgaaggcgca agaacaacag caggcacagc agcaaaatta taatactcaa cagcagcaag
      781 cgggctatga tcgcgccatg ggagcctgtc tcgaggggcg gcggttacac ggttcgatag
      841 gagaaatctg atcatgcgaa aattgtggct gggcggtctg ctgatcgctt atgcgtcatc
      901 gatcaaaccg tacttgccgc gagcgcaccg atcgacgatg ctagcccgtt actctgcgtc
      961 cccattgaga tgtacagctc tgtgaagttg aacaaaaaat taaagaagga ttgaaatgag
     1021 acatagctcg acaggaaaaa tgcgaacgtt agttttgctg ttgttatcat taactttgct
     1081 gcacgatccc atcccagtta gcgcgcagac cagtttcgaa cctcctgcga ccctcagcgc
     1141 atccaagatt cttccgccgg atcttctatc cggccccaat catcgggtgg aagaaagagt
     1201 ttataacgac gggtacctta atacttatcg ggtcggttca aagtttggaa ctttcgtcgc
     1261 tgtttcgacg ccgatgctgc gtaaacgaat cggcgaaatc aatgctctgg tgcgaatgga
     1321 gcagatacag gggaccaagg aatttacttc agcgctcaag gaagccggca cggatacact
     1381 ggtcggcttc aaaaacctag ttaccagccc cgtcgagacc gtaaaaggcg cggcttcggg
     1441 tctgggcgtg gcctttagac gagcaagcga cgcacttacg ggtccgaagc ggagcgactc
     1501 cgaagacagc aagctcaaag atctgatcgg cttttccaaa accaagcggg agtacgccta
     1561 ccaactcggt gtggacgcat acaccgataa cgagaaactg caagaccgcc tagatgagat
     1621 ttcatgggcg ggatatgccg gcggtattac ctgggccggc gccatggccg ccgttcccgg
     1681 aggcgccggg ttggcgatca cgatttctgg aacccacaaa ctcttgaatg aggtctttcg
     1741 caccacgccg ccggtcgatc ttcggcgtat gaatggtgaa aagctcaagg cgatggacgt
     1801 tcatcccgag gtcgctgatg ctttcttgaa caattcggtt tattcgccgc gggaccagac
     1861 actgctggtc aatgcgctcg aggagatgaa aggcgtcggc aatcgggcga ctttcgtacg
     1921 cttggcggcg tcaagcggag ataggaatat cgcgtttttc cgtgagcgcc aggcggaaat
     1981 gtacgccggc tttcataaag cggttgctgc ggttgaaaca tttatcgcgc tgggcgaatt
     2041 cgccgcggtt cgaaccacca aaaatgacgt tgttttcaat gtgccgctcg accatctcgt
     2101 ctggactcaa gacatggcaa aactgttgac cgccgcagac gcgcgcgtga cacaattaac
     2161 ccgt
//
LOCUS       DASULZ010000594         4626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_271336, whole genome shotgun sequence.
ACCESSION   DASULZ010000594 DASULZ010000000
VERSION     DASULZ010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271336"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="VFY96_12260"
     CDS             <1..945
                     /locus_tag="VFY96_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018040532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5607285.1"
                     /translation="EPDLPICDAHHHLWERPPNNYLLTHFLDDLASGHNVLASVAVEC
                     SYGYRKFGAGQLKPLGETEFLENVARQARSDARIETKVAAAIVGFANLSLGDAVAPVL
                     EGHLALSPSRFRGIRYSTTWDGSGAVRNEAPPRMLLDGHFRQGFGWLKKFNLSFDAWL
                     YHTQLPEFADLAREFPDVTIILNHIGAPLGVGPYAGKRDEVYQLWRQHIASLAEFPNI
                     VVKLGGVGSVRSGYDWHQRRIKASSEELAEILRPYFEVCIEKLGAERCLFEGNFPVEK
                     AANSYVILWNAYKRITKSYSASERAALFYGTAARVYRI"
     gene            complement(1184..2257)
                     /locus_tag="VFY96_12265"
     CDS             complement(1184..2257)
                     /locus_tag="VFY96_12265"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5607286.1"
                     /translation="MSVAGFNGEIGIHYPLHVLNRYSLPELIDLAARAAQSLGPFGFT
                     RVWTNDNLEYRSVLASSAAILARLPVKLGTAVAVPYFRNPIDFAMAFATMSELSSGRE
                     ISLGLGPGSRSILTRHVVRAKPLTIMAELAIALRTLFAGQTLHRDHIPVLAEYFHLNA
                     PHYALRFKAAAPIHLYYGPSLLKPAVLDLIAQHFDGVILQTLYGTDEMRESLERLERV
                     RTQSGAAEPLRKTMLINASIARDGETAREHAKRFVSHIVSGWPDEVLGAKDIDPKAIQ
                     AVRQAYAENRGVDAAAALTPDHAVDRLIIAGTAAQCRDRLAELFKLAAQHSFNEIVIG
                     VPLGPEIADVIDLWGSEILPGLR"
     gene            complement(2326..4242)
                     /locus_tag="VFY96_12270"
     CDS             complement(2326..4242)
                     /locus_tag="VFY96_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HEX5607287.1"
                     /translation="MKDTYDAVYSRWLNDPQNFWAEAAEAVHWDKKWNRVLDDSRPPF
                     YRWFPGGLLNTCYNALDRHVESGRANQPALIYDSPVTGTVRTFTYRELLDQVARFAGV
                     LLAQRVQKGDRVIIYMPMIPEAIVAMLGCARIGAIHSVVFGGFAAQELANRITDARPK
                     VIVSASCGIEAGRIIAYKPLLDKAIELAAVKPERCIIVQRSQEDASMIGGRDLDWSQE
                     MAYAKPAGCVPVGATDPLYILYTSGTTGMPKGIVRDNGGHLVALTWSMRHIYGVEPGE
                     VYWAASDVGWVVGHSYIVYAPLFNGNTTVLYEGKPVGTPDAGAFWRVVSQHGVKVLFT
                     APTAFRAIKREDPNGEFIRKYDLSGFRTLFLAGERCDPDTLLWAQKHLGVPVIDHWWQ
                     TETGWPIGANCVGLGMLPVKPGSCTKSVPGYNVQVLDDSGSELPPGQIGSIVVKLPMP
                     PGCLPTLWNNDEGYRKAYLARFPGYYLTADAGYKDEEGYLWVMTRTDDIINVAGHRLS
                     TGALEEVLASHPDVAECAILGVADAIKGEIPLGLIVLKAGVRRSDGEIVGETIQMVRE
                     RIGAVASFRVATVVKRLPKTRSGKILRGTIKKIADGVEYRVPPTIDDPVILDEITADL
                     SALGYPAGAARSSV"
BASE COUNT          963 a         1310 c         1394 g          959 t
ORIGIN      
        1 gaacctgatt tgccgatctg tgatgcgcat catcatttgt gggagcggcc gcccaataat
       61 tatctcttga cccactttct cgacgatctc gccagcgggc acaatgtcct cgccagtgtc
      121 gcggttgaat gcagctacgg ctatcggaag tttggcgccg gccagttaaa gccgctcggt
      181 gaaaccgagt ttttggagaa tgtcgcgcgg caggctcggt ccgatgcgcg gattgaaacc
      241 aaggtggcgg cagcgattgt cggtttcgcg aatcttagct tgggcgatgc tgtggcgcct
      301 gtgctcgaag ggcacctggc gctgagcccg agccggttcc gcggtatccg ttattcgacg
      361 acgtgggacg gaagcggtgc ggttcggaac gaggcgccgc cgaggatgct tctcgatgga
      421 cacttccgcc agggattcgg ctggctgaaa aaattcaatt tgagcttcga tgcctggctg
      481 tatcatacgc agcttccgga gttcgccgat ctggcccggg agttccccga cgtcaccatc
      541 attctcaatc atatcggtgc gcctttggga gtcggaccgt atgcgggaaa gcgcgatgaa
      601 gtttaccagc tttggcgcca gcacatcgcg agtcttgccg aatttccgaa tatcgtggtg
      661 aagcttggcg gggtgggctc ggttcgttca ggctatgact ggcaccaacg acggatcaag
      721 gcgtcttcgg aagagctggc ggaaattctg cggccctact tcgaggtatg catcgaaaag
      781 ctcggcgcag agcgctgcct cttcgaaggc aacttccccg tcgagaaggc ggcgaactcg
      841 tatgtgattt tgtggaacgc ctataagcgc attacgaaaa gttattcagc gtcggagcgt
      901 gcggcactgt tttacggcac ggcggcgcgc gtttatcgca tttaaggaga gcgcgcaaaa
      961 catgtcgagt gagaaaacga ataaccgaga aagatttctg gctgcggcag agtatgtttg
     1021 ttatttcgag gcgaagccga gaaatcttat gttcctaact aactgaaaat gcaaaatctc
     1081 tcactatctt cgagatgaca aatcggcatc aatgagtttc tcacagtctt acgctcgaat
     1141 gacgcgtttg taacacgaat gcttccgcag cttgtgattt caatcagcgc aggccgggta
     1201 gaatttcgct gccccaaagg tcgatcacat cggcaatttc gggtccgagt ggcacaccga
     1261 taacgatttc gttgaagctg tgctgggctg cgagcttgaa tagctccgca agccgatcgc
     1321 gacattgtgc cgccgtgccg gcgatgatga gccggtccac cgcgtgatcg ggcgtgagcg
     1381 cggcggcggc atcgacgccg cgattttccg cgtaggcctg gcgcacggcc tgaatcgcct
     1441 tgggatcgat gtctttcgcc ccgaggactt cgtcaggcca acccgagacg atatgagaaa
     1501 cgaaccgctt cgcgtgctct ctggcagtct cgccatcgcg cgcgatcgat gcgttgatca
     1561 acatcgtttt gcggagcggc tccgcggcgc ctgattgagt tcgtacccgc tcgagccgtt
     1621 cgagggactc gcgcatctcg tctgtgccgt aaagcgtttg caggatgaca ccatcaaagt
     1681 gttgcgcgat aagatcgagg acggcgggct tgagcagact cggcccatag tagagatgaa
     1741 tcggtgccgc cgctttgaag cggagcgcat agtgtggcgc gttcaagtgg aaatattcgg
     1801 cgagcaccgg aatatggtct cgatggagag tctggccggc aaagagagtg cgcagcgcga
     1861 ttgccaattc cgccatgatg gtcaacggct ttgcgcgcac gacgtggcgg gtcaaaatcg
     1921 atcgcgagcc cggaccgaga ccaagagaaa tttcccgccc gctcgaaagc tcgctcatgg
     1981 tcgcaaaagc catcgcgaag tcgatgggat tacgaaagta gggcacagcg acggcagtgc
     2041 cgagcttgac gggcaagcgc gccagaatgg cggcagagct cgccagcacg ctccgatact
     2101 cgagattgtc attggtccag acgcgggtga agccaaacgg accgagactc tgtgcggcgc
     2161 gcgcggcgag gtcgatcagc tcggggagcg aatagcgatt aaggacatga agcgggtaat
     2221 gaatgccgat ttcaccattg aatcctgcga ctgacatgag ttcgggttaa cacaccggct
     2281 tccgataagt caaagatagg ctcaaaacct tcaagcaggc ataaatcaca ccgagcttct
     2341 tgctgcaccg gcgggatagc ctaacgcgga caaatcggcg gtgatttcat cgaggatcac
     2401 cggatcgtca atggtgggcg gaacccggta ctcgactccg tcggcgatct ttttgatggt
     2461 accgcggaga atcttgccgg agcgcgtctt cggcagccgc ttgaccaccg tggcaactct
     2521 gaaagatgcc accgcgccga tcctttcgcg caccatctga atcgtttcgc cgacgatctc
     2581 gccgtctgaa cgtcgcacgc cggccttgag cacgatcagg cccagcggaa tttctccttt
     2641 gatcgcgtcg gcgactccca gaatcgcgca ttcggcgacg tcgggatgcg acgccagcac
     2701 ctcttccagt gccccggtgg agagccgatg accggcaaca ttgatgatgt cgtcggtgcg
     2761 ggtcatcacc caaagatagc cctcttcgtc tttgtatccc gcgtctgcgg tgaggtagta
     2821 gccgggaaac cgggcgaggt aggctttgcg gtaaccctcg tcgttattcc acagtgtcgg
     2881 aagacaaccc ggcggcattg gcagcttgac gacgatgctg ccgatctgac ccggcggcaa
     2941 ttcgctgccg ctgtcgtcca atacctgaac attgtaaccg ggcactgact tcgtacacga
     3001 tcctggtttt accggaagca ttcccagtcc gacgcaattg gcgccgatcg gccaacccgt
     3061 ctcggtctgc caccagtggt cgattaccgg tacgccgaga tgcttttgcg cccacaacag
     3121 cgtgtccgga tcgcagcgct cgccggcgag aaagagcgtg cggaagccgc tcaagtcata
     3181 cttgcggata aattcaccgt tgggatcttc gcgtttgatc gcgcgaaacg ccgtgggcgc
     3241 cgtaaacaag actttcacgc cgtgctgtga gacgacccgc cagaacgcgc ctgcatcggg
     3301 agtgccgacc ggtttgccct catagagcac cgtcgtattg ccgttgaaca gcggcgcata
     3361 gacgatgtag gagtgaccga cgacccagcc cacgtccgac gccgcccagt acacttcacc
     3421 cggctccaca ccgtagatgt gccgcatgct ccatgtgagc gcgaccaagt gcccgccgtt
     3481 gtcgcgcacg attcctttgg gcatgccggt cgtaccggag gtgtaaagga tgtaaagcgg
     3541 atcagtagcc ccgacaggaa cgcagcccgc cggttttgcg tacgccatct cttgcgacca
     3601 atcgagatcg cgcccgccga tcatcgacgc gtcttcctgc gaacgttgga caatgatgca
     3661 gcgctccggc ttcaccgccg caagttcgat cgctttgtcg agtaacggct tgtaagcgat
     3721 gatgcggccg gcttcgatgc cgcacgaagc cgacacaatc accttcggtc gcgcatcggt
     3781 gatgcgattg gcgagctcct gggcggcgaa gccgccgaag accacagagt gaatcgcgcc
     3841 gatgcgcgcg catcccagca tcgcgacgat cgcctccggg atcatcggca tgtaaatgat
     3901 gacccgatcg cctttctgca ctctttgcgc gaggagcacg ccggcgaatc tcgcgacttg
     3961 atcgagcaac tcgcgatacg tgaacgtccg gacggttccc gtcacaggac tgtcgtagat
     4021 gagcgccggc tgattcgccc ggccgctttc gacgtggcga tcgagcgcat tgtaacaagt
     4081 gttgagcaat ccgccgggga accatcgata gaacggcggc cgcgagtcgt cgagcacccg
     4141 gttccacttc ttatcccaat gaaccgcttc ggccgcctcg gcccagaaat tttgcggatc
     4201 gttcagccag cggctgtaga cggcgtcata ggtatctttc atggtggccc cccgatctag
     4261 tcggattcca ttcttatctt cggcgatcaa tctgcgaccg accctatgcc gacgagcagg
     4321 tccggtgcaa gcaggcggcg atgtggttgg atggagcgtg agaggaagcg tcgtgcgggc
     4381 agaatcttga cgaagaatca gctcgcgagt tacttgtaac gtggttgttg cgcaggggac
     4441 caagaatgaa ctcaaaatct gaagttctaa attgttgaag atcacacgtc ggttgcttcc
     4501 ggtattctgc tggctgatgc tcggcgtttg ttgttttccg cccgcgctca gtcggcgaag
     4561 caagctgaga agaaactgca cggcagttgg acggcgacaa aagcggaacg cgatggcaaa
     4621 gccgcc
//
LOCUS       DASULZ010000595         2818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_271372, whole genome shotgun sequence.
ACCESSION   DASULZ010000595 DASULZ010000000
VERSION     DASULZ010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271372"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VFY96_12275"
     CDS             complement(<1..198)
                     /locus_tag="VFY96_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6755 family protein"
                     /protein_id="HEX5607288.1"
                     /translation="MSGSRFARHQRRTIINGILSVVSILVILQLWLLTATMNAYLGAD
                     DSILVPAALASMICLFLNFGLL"
     gene            complement(195..743)
                     /locus_tag="VFY96_12280"
     CDS             complement(195..743)
                     /locus_tag="VFY96_12280"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX5607289.1"
                     /translation="MDDRDPQTMTIAPDRRLDSEQPRWRQDFPIDWPEDEYVARREFT
                     KFMVLTSLAFAIGQLWIVIENFFRQRNAPGPVQEIAGVDDLPVGGSLVFDYPKAHQPA
                     VLIRMDAERFIAYGQKCTHLSCPVLPRVDEGRLHCPCHEGSFDLATGRPVAGPPRRPL
                     ARVTLQFRQGRIYATGVEERTT"
     gene            complement(753..1391)
                     /locus_tag="VFY96_12285"
     CDS             complement(753..1391)
                     /locus_tag="VFY96_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5607290.1"
                     /translation="MTTTQPMEFFIDPRRCIGCQACVQACSECDTHKGQSMIQLDYID
                     RVNSTQTAPVVCMHCDSPTCAEVCPADAIKKTADGVVQTARKPRCIACNNCVIACPFG
                     VPKMNTGMNLMMKCDMCYDRTSVGKKPMCASVCPSQALFFGTREEIEKLRPRSHPIQR
                     FHFGDQTITTKVNMMVPKESRQEDLDVTAALYESASTDTPADDPMMANLQPE"
     gene            complement(1400..>2818)
                     /locus_tag="VFY96_12290"
     CDS             complement(1400..>2818)
                     /locus_tag="VFY96_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEX5607291.1"
                     /translation="IGQETVGFEDVAAHVRDWTPRKTAEITGIAERAVRQAAEWWGTA
                     KKSFLMHARGIEHHSHGVANVLGAINMVLASGRIGREGCGYATITGQGNGQGGREHGQ
                     KCDQLPGARDISNPEHRAYIAEIWGMNADDIPSAGVDAYEIFRKIHAGEIRGVLSLCF
                     NPMVSLPDNNFIREALEKLEFYAVIDFFLSDTARFADIVLPGSLHEEDEGVVTTAEGR
                     VIKINQAVDCPGEAHQDWRIIQDIAKALGRAQGFSFASPEEIFRELRRASRGGIADYS
                     GITYEKVEQHYGVFWPCPHEDHVGTPRLFEPGSWNPNAKGSGRFYFFDGKARFNLACY
                     TPPTEEIDAQYPIILTTGRVVSQFLSGTQTRRIGPLVDHYPEPRVELHPLLAERLQIA
                     DGDWVVIESRRGNCTLRAQVVKTIRPDTIFVPYHWGGKKSVNQLTIAAQDPISKIPEY
                     KVCAVRLRKAAAVPVGDLEPEP"
BASE COUNT          582 a          831 c          805 g          600 t
ORIGIN      
        1 gagtaggccg aagttcagaa acaggcagat catgctcgcc aacgccgccg ggactaagat
       61 cgaatcatcg gcgccaaggt aggcgttcat ggtcgcggtt aaaagccaga gttgcagaat
      121 taccaggatg ctcaccacgc tcagaatccc gttgataatc gttctgcgct gatgccgggc
      181 aaagcgcgat ccgctcacgt ggttcgttcc tcgacgccgg tggcatagat ccgcccttgg
      241 cgaaactgca gcgtgacacg cgctagcgga cgccgcggcg gaccggccac cgggcgtccg
      301 gtggccagat cgaaactgcc ctcatgacag ggacaatgaa gccggccttc gtcgacacga
      361 ggaaggaccg gacaggagag atgtgtgcac ttctggccat aggcaataaa ccgctcggca
      421 tccatccgta tcagtaccgc cggctgatgc gctttgggat aatcgaatac cagcgagccg
      481 ccgacgggaa gatcatccac gccggcaatt tcctgcaccg ggcccggcgc gttccgctgg
      541 cgaaagaagt tttcgattac gatccatagc tgaccaatgg caaacgccaa actggtgagc
      601 accataaatt tagtgaactc gcgccgggcg acgtattcgt cctcaggcca atcgatcggg
      661 aaatcctggc gccagcgggg ttgctccgaa tcgagccggc ggtcgggcgc aatggtcatc
      721 gtctgcggat cacgatcgtc catagtctgc tactattcgg gctgcaggtt cgccatcatt
      781 ggatcgtcgg ccggggtatc ggtgctggca ctctcgtata gcgccgccgt cacatccaga
      841 tcttcctgcc gtgattcctt tggcaccatc atgttcacct tcgtcgttat cgtctggtcg
      901 ccgaaatgaa atcgctggat gggatgcgag cgcggtctga gtttttcgat ttcctcccgc
      961 gtgccgaaaa atagcgcctg gctcggacag accgacgcgc acatcggctt tttaccgact
     1021 gaggtccgat cgtagcacat atcgcacttc atcatgagat tcatgccggt attcattttg
     1081 ggcacgccga acggacaagc gatgacgcag ttattgcagg cgatgcagcg cggtttgcga
     1141 gccgtttgca cgacaccgtc ggcggttttt ttgatggcgt ctgccgggca aacttccgcg
     1201 catgtcggtg agtcgcagtg catacagact accggagccg tctgagtgga atttactcga
     1261 tcgatataat cgagctggat catcgattgc cccttgtgcg tatcgcattc actgcatgcc
     1321 tggacgcagg cctggcagcc aatgcagcgc cgcgggtcga tgaaaaattc catgggctgt
     1381 gtcgtcgtca tcacggactc tacggttcgg gttccaaatc tccgacgggc accgccgccg
     1441 cttttctgag tcgcacagcg cagactttgt actcgggaat cttcgagatc ggatcctgcg
     1501 ccgcaatcgt cagctggtta acgcttttct tgccgcccca gtgataagga acaaaaatcg
     1561 tatcaggacg gatcgtcttc accacctgtg ctctgagcgt gcaattgccg cgtcgcgatt
     1621 cgataacaac ccaatcgccg tcagcgatct gcagccgttc ggccaagagc ggatgaagct
     1681 cgacgcgcgg ctcgggataa tgatccacga gcgggccgat ccgccgcgtc tgtgtgccgg
     1741 aaagaaactg gcttaccact cgcccggtgg tgagaataat tggatactgg gcatcgatct
     1801 cttcggtggg aggggtatag caagcgagat tgaaacgcgc tttgccgtcg aaaaaataaa
     1861 acctgccgct acctttagca ttcggattcc atgaacccgg ttcgaagagt ctcggcgttc
     1921 cgacgtgatc ttcgtgtggg cacggccaaa aaacgccgta gtgctgctcc actttttcat
     1981 aggtaatccc ggagtaatcg gcgataccgc cgcgcgaagc tcggcgcaac tcgcggaaaa
     2041 tttcctcggg agaagcgaag ctaaatccct gcgcgcgccc gagcgcttta gcgatgtctt
     2101 gaatgatacg ccaatcctgg tgcgcttcgc cgggacaatc gaccgcctga ttaattttga
     2161 tcacccggcc ttccgcagtc gtgacaacgc cttcgtcttc ttcgtggagc gacccgggca
     2221 ggacaatatc ggcgaaccgg gcggtgtcgc tcagaaaaaa atcgatcacg gcgtagaatt
     2281 cgagcttttc cagcgcctcc cgaatgaagt tgttgtccgg cagtgacacc atgggattga
     2341 aacaaagcga caggacaccc ctgatctcac ccgcatggat cttgcggaaa atttcgtagg
     2401 cgtcgacgcc cgcggacgga atgtcgtccg cgttcatgcc ccaaatttcc gcgatgtaag
     2461 cgcgatgttc gggattggaa atatcgcggg ctcccggaag ctggtcgcat ttctggccat
     2521 gttcccgccc gccctggccg tttccctggc cggtgatagt cgcataaccg cagccctcgc
     2581 gaccgatccg ccccgaagcc aacaccatat tgatggcgcc gagcacgttg gcaacgccgt
     2641 gactgtgatg ctcgatgccg cgcgcatgca taaggaaact ctttttcgca gtgccccacc
     2701 actcggccgc ctggcgaacg gctcgctccg caattccggt gatctcggcg gtttttcgcg
     2761 gcgtccagtc gcgcacgtgc gcggccacat cttcgaagcc gacggtttct tgtccgat
//
LOCUS       DASULZ010000596         2416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1489239, whole genome shotgun sequence.
ACCESSION   DASULZ010000596 DASULZ010000000
VERSION     DASULZ010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489239"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..500
                     /locus_tag="VFY96_12295"
     CDS             177..500
                     /locus_tag="VFY96_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607292.1"
                     /translation="MELKKQGESKMDLEKAKLNPAAVFKPLKKSFQAKNCRANKNLKS
                     FGDGNQDGMSMEVGEEEGMPGPQPKLLQRFATLCSPRLLAGYRALEPQQSDYFGPCDE
                     RPSKR"
     gene            complement(634..891)
                     /locus_tag="VFY96_12300"
     CDS             complement(634..891)
                     /locus_tag="VFY96_12300"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX5607293.1"
                     /translation="MARPLRLQFPGAIYHVTSRGNSRQKVFFTDADRELFLRTLSGVV
                     TRYGWICYAYCLMANHNHLLVETPKANLSIGMRQLNSRQSV"
     gene            1204..2028
                     /locus_tag="VFY96_12305"
     CDS             1204..2028
                     /locus_tag="VFY96_12305"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5607294.1"
                     /translation="MPAGACDTHLHVYGPFDRYPLSPDRHYDPAPDCTLDDYLKVHRT
                     LGLERAVIVTGSANGTNNQITLDTLARMNGSFKAIALLGADVSDAELHRLKAGGFSGF
                     RIKSSGRGYTLLKDTQRMVERTREFDWHVEFMVQSLQEAIATTAFLSSLKVPYVFDHV
                     AHAVPDQHKHGSKEFAELLAILKNEEHAWINLYSFYQLSKSGSPDYADMVEVIQAIIA
                     ARPDHVIWGSNWPHPSISVPTPNDGDLLNFLLAAAPEEHARTRILSENPAKLYGWL"
BASE COUNT          601 a          670 c          629 g          516 t
ORIGIN      
        1 gccgcgccaa ggaagtgggc atcctgaccg aggggccatg agcacgacga gcaagtagtt
       61 agcgcgggcc aagccctggc aattccgtct catattgaac aattggttct gctacagagc
      121 gccggtggac tgttaggcga ctcaacttga ttcccaatgt tctatcagcg gtaatcatgg
      181 aattaaaaaa gcagggagaa agcaaaatgg atttggaaaa agcaaagctg aatcccgcag
      241 ccgttttcaa acccctcaag aaatcgtttc aagccaagaa ttgtcgcgcg aacaaaaact
      301 tgaaatcctt cggcgatggg aaccaggacg gaatgtcaat ggaagtcggg gaggaagaag
      361 gcatgcccgg cccgcaaccc aaactgctcc agcgattcgc gacgctctgc agccctcgac
      421 tattggccgg ataccgagca ctcgagcccc agcaaagcga ttatttcggg ccgtgtgatg
      481 aacggccttc gaaaagatga gaccacgtgc cctacagtca gcctgtaaga tcaggctttt
      541 tattctagac aatcctcggc caatcacgca ttttcacccg gtcggaccgc gctatttctg
      601 aagcttactg ctcggcagta cgcgcgaatc gacttaaacg gattgacgcg agttgagttg
      661 gcgcatgcca atcgaaaggt tcgccttggg cgtctccacc agaagatgat tatgattggc
      721 catcaggcag taggcgtaac agatccaacc gtaccgagtg acaacacccg acagagtgcg
      781 cagaaacagc tcgcggtcgg cgtcagtgaa aaacactttc tgccgagaat tgccgcggct
      841 agtcacatga tatatcgcac cggggaactg caatcgaaga gggcgcgcca tgacgtgccg
      901 taatttatcc gacccaaatg tgttattgca agacctgacc ccaataggcc tggcgtcaga
      961 tgataaatcg caccgggaaa ttctaatcgt agagggcgcg ccatgacgtt ccgtctgtat
     1021 ccgacgaaat gtgctaattg caagtcctga ccccaacaag tgacgcggag cgcgtgtcga
     1081 caggattgaa ctttgaagtc gaaatgcgga acgttgaacc gtccgaggcg ctgaattaga
     1141 gtggggcgca tcatgcgtac cgcaacaccc ccagatcgca atacgcgacc gccgcgattc
     1201 aaattgcccg ccggagcgtg cgacacgcac ctacatgtgt acggaccgtt cgatcgctac
     1261 ccgctttcac ccgaccggca ctatgatccc gctcccgact gcactctcga tgactatctc
     1321 aaggtgcatc ggacgctcgg attggagcgg gcggtgatcg tcaccggcag cgccaacggc
     1381 acgaacaacc aaatcacgct cgatacattg gcgcgcatga atggcagttt caaggccatc
     1441 gctttgttgg gtgctgatgt tagtgatgcc gagctgcaca gactcaaagc cggtggtttt
     1501 agcggctttc gcatcaaatc ctccgggcga ggctacacgt tgttgaaaga cacacagcgg
     1561 atggtcgagc gcacgcgaga gttcgactgg cacgtggagt ttatggtgca atcgctgcaa
     1621 gaggcgatcg ccaccaccgc ttttctaagc tcactcaaag tgccgtacgt cttcgaccac
     1681 gttgcgcacg cggtgcccga tcagcataag cacggcagca aagaattcgc ggagctgctg
     1741 gcaatcttaa aaaacgaaga gcacgcctgg atcaatctct acagcttcta ccagctgtca
     1801 aagtcgggct cgcccgatta cgccgacatg gtagaagtca ttcaagcgat catcgctgcg
     1861 cggcccgatc acgtcatctg gggcagcaac tggccgcacc cgagcatcag cgtgccaact
     1921 cccaatgacg gagatctgct caattttctg ttagcggcgg cgcccgagga acatgcgcga
     1981 acaagaatct tgagtgaaaa cccggcaaaa ctgtacggct ggttatagat ccgacgtgac
     2041 aaaacttttt ttcccctgac cagttcggac accgtcatat cttggaacgc accaacctat
     2101 gttccagagt cgtaaacgtt tccgttccac aggaaagtgt tttccgtgcc tggaacttta
     2161 ttttctgcga tagaattgcg tgtgtaagct tcgcttgctt cgcgcagcgc atacgcctct
     2221 ctagcaactc aacctctccc ctttttacga cgtgattgac accttctccc gtcggcgata
     2281 tttctttcgc aagagattga gagtgtttca aatttcggac ttggagctcg gcgtacataa
     2341 tcgttgcgaa tatattttca tacgagccgt gctcgcagaa tctaacagga cgctcattcg
     2401 taccgtcctt ctagcc
//
LOCUS       DASULZ010000597         3110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1185548, whole genome shotgun sequence.
ACCESSION   DASULZ010000597 DASULZ010000000
VERSION     DASULZ010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185548"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            505..1401
                     /locus_tag="VFY96_12310"
     CDS             505..1401
                     /locus_tag="VFY96_12310"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5607295.1"
                     /translation="MMRGGIIMETNLPVEVVRRQNWLEPIAERLQPAVAAVLDDTIGV
                     EGAKILHGTWLGHPLHVVLTDVPLGSWTAAAMFDLLDIANGNRAVRRAADSAISVGLF
                     AATLTAITGLADWSKIGGGESRRVGLAHGLLNVTATACYLTSLCLRRNRSRPVGRQFA
                     FLGLIVSTFSAYLGGHLVYKKKLGVDHTADTSSPENFMPVLAEAALPENELKRVNADG
                     MPVLLVRKGPRIYAIAETCAHLGAPLSEGKLEGTSVRCPWHGSRYSLEDGRVLEAPSV
                     HAQPVLEVRIRDGQIEVRKSND"
     gene            1534..2610
                     /locus_tag="VFY96_12315"
     CDS             1534..2610
                     /locus_tag="VFY96_12315"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5607296.1"
                     /translation="MKIAQVTSVYISVPPKTYGGTEWIVHYLCRDLGRRGHQVELFAS
                     GDSKVDCTLQSVLPIASQDDPRSTFYLEKEYEARNTWNLYRQGERFDVIHAHWTTMAP
                     YFSSFTTTPTLITYAYIERELHEYYKNYFPRCFPVCVSRAQAKMLGDESIPVVYNGLD
                     VDDVPFGDEPEDFFLIAGRMVPEKGIADAIRIATKNKAKLVIVGHVTSHLPWSERYFS
                     QEVKPHIDGDKIRYIERLPHCDLARLMSKAKAFLFPIKWDEPFGMVVVEAMAAGAPVL
                     AYARGAMPELIKHGETGYLVESEDDMVAAMQRLDALDRGRCRAWVREHFSVEQMIDGY
                     EILYKNVVRGPRGRKIDSQSNRVL"
     gene            complement(2804..>3110)
                     /locus_tag="VFY96_12320"
     CDS             complement(2804..>3110)
                     /locus_tag="VFY96_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607297.1"
                     /translation="RNRKGAEPAAQIKQGPRVFQWQLIEHLRRDLLSIEVQSAKQSSR
                     FVMFGEKWSVDFRSFFAPKSRGELAPSLPRVKFGKQKTRAMTGKMSGRKSSLFSAFV"
BASE COUNT          679 a          847 c          893 g          691 t
ORIGIN      
        1 aatgtcacga acggattctc atgggtgaga tgatccggaa cccagagccg agcgaacggg
       61 tactgagaca tcgcctgctt cgcgtactcc gccgtctgcg gagccggagg caacgggtag
      121 aattgcatgc cgaaacgcat gttacgacta ctttgttact tgaatctcga acgactcggc
      181 gaaaacgcga ttatcatggc ggcgccgcaa gatcaatgtg ttcgccgagc gacatcactc
      241 agcaagcagc gcgtgattcc agttccgcat tgtccgtgga cagagggtgc gcgcgtcgtt
      301 gggaaatttc tatcgcaatc acataaatat ttggatgcca aaacctgacc tcaggttgtc
      361 cttcttatca ttgctagccg tcgcgcacct cgtcgaggcg caagacccgg tcgaagacgc
      421 ggccgctcga tcttgccttc gagaacgtcc aggacaagct ccttataatc agatcccctt
      481 gatcgaagct gcaagagcaa tatcatgatg cgagggggta tcattatgga aaccaactta
      541 cccgtagaag tagtcaggcg gcagaactgg ctggaaccga ttgcagaacg tttgcagccg
      601 gcggttgcgg cagtgctcga cgatacgatt ggtgtcgagg gcgcgaagat tttacacggc
      661 acttggttgg ggcatccgct gcacgtggtc ctcacggacg tgccgctcgg ttcttggacc
      721 gccgctgcca tgttcgacct tcttgatatc gcgaatggaa acagagcggt gcgacgagca
      781 gcggattccg cgatttcggt aggtctgttt gccgcgacgc taacagcgat caccggactg
      841 gcggattggt ccaagatcgg cggcggcgaa tcgcgccgcg tcgggctcgc ccacgggtta
      901 cttaatgtca cggcgacggc ctgttacctg acgtcgctgt gccttcgacg caatcgttca
      961 cgaccggtcg gacggcagtt tgcgtttctc ggattgatcg tctcgacttt ttctgcctat
     1021 ctcggtggac acctggtcta taagaaaaaa ctcggtgtcg atcacaccgc ggacacttca
     1081 tcgcccgaga actttatgcc ggtcttggcc gaagccgctc tacccgagaa cgaattgaaa
     1141 cgcgtgaacg ccgatggcat gcccgtcctg ctcgtgcgca aagggccgcg catatacgcc
     1201 atcgccgaaa cgtgcgccca ccttggcgca ccgctttcag agggaaagtt agaaggcact
     1261 tcggtacgct gcccatggca cgggtcgcgg tattcgctgg aagacggccg cgtgctggaa
     1321 gcgccatcgg tgcatgctca accggttttg gaagtgagga tacgcgacgg tcagatcgag
     1381 gtccgtaagt cgaacgactg atttccctga tgttgatcga gacgatcgag cggaatcggt
     1441 ctttgtattt cagacgcaaa tagcagggca ggcaagccgc cgtcgctcga cccacgcgat
     1501 ccgtcgtgat gtgttgacag gcggccgcat ccgatgaaga tcgctcaggt cacgagcgtt
     1561 tatatttccg ttccgccgaa aacttacggg ggaacagagt ggatcgtcca ttatctttgc
     1621 cgggatctcg ggcggcgcgg tcaccaagtg gagctgtttg cttccggaga ttccaaagtc
     1681 gactgcactc tccagtccgt cctgccgatc gccagtcagg acgacccccg ttcgactttt
     1741 tatttggaaa aggaatacga ggcgcgcaat acctggaatc tttatcgtca gggtgaacgc
     1801 ttcgatgtga ttcatgctca ttggacgacg atggcgccgt atttttccag ttttacgacg
     1861 acacccacgc tgatcaccta cgcctacatc gagagagaac tgcacgagta ttacaagaat
     1921 tattttccgc gctgctttcc ggtgtgcgtg agcagggctc aagcgaaaat gttgggcgat
     1981 gaatcgatac ccgtcgttta caacggcctg gacgtcgacg atgttccgtt cggcgacgag
     2041 cccgaagatt ttttcttgat cgccgggcgg atggtgcccg aaaaaggtat tgccgatgcg
     2101 atacgcattg caacgaaaaa caaagccaag ctcgtgatcg tgggccacgt gacctcgcac
     2161 cttccttgga gcgaacggta tttttcgcaa gaagtaaagc cgcatatcga tggagacaag
     2221 attcgctaca tcgagcggct gccccactgc gatttggcgc gactgatgag taaggccaaa
     2281 gcttttcttt ttcccattaa gtgggacgag ccattcggaa tggttgtcgt cgaagcgatg
     2341 gccgccggag cgccggtgct ggcctacgcg cgaggcgcca tgcccgaatt gatcaagcat
     2401 ggcgagaccg gctatttggt cgaaagcgaa gacgatatgg ttgcagcgat gcagcggctc
     2461 gacgccttgg atcgaggacg ctgtcgcgcc tgggttcgag aacacttctc cgttgaacag
     2521 atgatcgacg gctacgaaat cctttataaa aacgtcgtgc gaggtccgcg aggccgaaag
     2581 atcgattccc agtccaaccg tgtcctctag caagtggagc cttgatcgtc tccgaagcga
     2641 gagcgcttac tggcgctcgt gcgttcttat taccgcagaa cacatcggtc tattcgcccg
     2701 gattggaaag cagcaaaaga acgcggttgc actggcggcg catttcggcg gaaatccagc
     2761 cgattgggaa atcttttgca acgctctctg cgccatgggc ttgctacaca aacgcggaaa
     2821 acaaactgct ttttcgtccc gacatcttgc cggtcatggc gcgagttttt tgcttcccga
     2881 atttgacgcg tggaagagat ggggcgagct cgcctctgct cttcggagcg aaaaaagacc
     2941 gaaaatccac cgaccatttt tctccgaaca tgacaaagcg cgaagactgc ttcgcgctct
     3001 gcacctcgat gctcaggaga tcgcgcctca agtgctcaat aagttgccat tgaaacactc
     3061 gaggtccttg cttgatctgg gcggcgggct cggcgccttt tcgattgcgt
//
LOCUS       DASULZ010000598         2491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_372471, whole genome shotgun sequence.
ACCESSION   DASULZ010000598 DASULZ010000000
VERSION     DASULZ010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372471"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VFY96_12325"
     CDS             <1..440
                     /locus_tag="VFY96_12325"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX5607298.1"
                     /translation="EMAHAYLGHIAYARAKQVIEMALSMASEVLVPGAGRAAVLLAEL
                     ATKKFDRDQEREADLFGLIWAHRSGFDVSAAKDLWRRMAIEIPESVESGFLSSHPSSA
                     ERFRSIERVADMLKEGVDPLRSFASARPSGRDWFRQSGWDWLR"
     gene            complement(480..881)
                     /locus_tag="VFY96_12330"
     CDS             complement(480..881)
                     /locus_tag="VFY96_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607299.1"
                     /translation="MPEPPQFTAEASLVNTTHLIYALHALSLLIGITTAATIIGAFVF
                     GVPSIIAVVINYLKREEARGTYLESHFRWQIRTFWFAVLWCVIGTMLIVTFVGIPLAL
                     AIFFGIGIWVIYRIGRGWLALRDRRPMYAAV"
     gene            complement(951..1970)
                     /locus_tag="VFY96_12335"
     CDS             complement(951..1970)
                     /locus_tag="VFY96_12335"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013044256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX5607300.1"
                     /translation="MKTDDLRIVKTRPLLSPAILAEEIPLTEAASENVYRSRRAIEAI
                     LDGKDHRLLVVVGPCSVHDTKAALEYATKLKPIVDALSSALLIVMRVYFEKPRTVVGW
                     KGLINDPDLNESYQINKGLRLARQLLADIVELGVCAGTEFLDTTFGQFYADLISWGAI
                     GARTAESQVHRELASGLSMPVGIKNRTDGNVQVAVDAIQAARSRHLFPSLTKEGAPAI
                     LETAGNPYAHLVLRGGSQTGPNFDHASIEAAVRLLRTAGLPDVLMVDCSHGNSERDAT
                     RQIEVAKSIMKNLGGSAVRALMLESHLVAGRQDKPVTYGQSITDPCLGFSETETLLDA
                     LARAV"
     gene            complement(1991..>2491)
                     /locus_tag="VFY96_12340"
     CDS             complement(1991..>2491)
                     /locus_tag="VFY96_12340"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX5607301.1"
                     /translation="LKINPRGKVPSLDADGKILVENTAILTYLARRFPEKKLMPSDPV
                     EEARCIATMCWFSSIVHPSFQRCMRPERFADSEAAHAAVKENGRKSFWANCQEINSMI
                     EGHDWIMGRDFTVVDGYALVFYGWGLRGGFPMKELGAYTAWQERIMNRPTVRKTVESE
                     QSVSTS"
BASE COUNT          542 a          700 c          718 g          531 t
ORIGIN      
        1 atgagatggc gcacgcctat ttgggacaca tcgcttatgc gcgggcgaag caggtgattg
       61 aaatggcttt aagtatggca tccgaggttt tggtgcccgg cgcgggtcgg gcagccgtgc
      121 ttctggcgga gctcgccacg aaaaaattcg accgtgatca ggagcgcgag gccgatctct
      181 ttggcctgat ctgggcgcat cgttcaggat tcgatgtgag cgcagccaaa gatctgtggc
      241 gcagaatggc gatcgaaata ccggaaagcg tggagagcgg gtttctctcc tcccacccaa
      301 gttccgcgga acgatttcgc tccatcgagc gggttgcgga catgctgaag gagggtgtcg
      361 atccgctgag gagttttgcc tccgcccggc cgagcggtcg cgattggttt cggcagagtg
      421 gatgggattg gctgaggtaa tcttttcccg gaacggtcgc tcacgcccag gatcatgaat
      481 caaaccgctg catacattgg ccttcgatct ctgagcgcga gccaaccgcg ccctattcga
      541 tagatgaccc aaataccgat cccgaaaaaa atcgctaagg ctagcggaat cccgacaaac
      601 gtcacaatca gcatcgtccc gattacgcac cacaggacgg caaaccaaaa agtacgaatc
      661 tgccaacgaa agtgagattc gagataggtg ccgcgcgcct cttctctttt taaataattg
      721 atgacaaccg cgataatcga cggcacaccg aaaacgaatg cgccgataat cgtggctgcg
      781 gtagtaatgc cgatcagcag gctgagcgca tgcagcgcgt agatcagatg ggtcgtattg
      841 accagcgatg cttctgcggt gaattggggc ggctcgggca tgacttgctc aggaagatca
      901 cagcgagact aaatcacagt tacggctcca aagtctctca ctaacagcca ctaaactgcc
      961 cgcgcaagag catcgagtaa tgtttccgtc tcggaaaacc ccaggcacgg atcggtgatg
     1021 ctctgaccat aggtaaccgg cttatcctga cgaccggcca caagatggct ctcgagcatc
     1081 agcgctcgaa ccgcggagcc accaaggttc ttcataatag atttcgcgac ctcgatctgg
     1141 cgggtcgcat ctctctcgct gtttccatgg ctgcaatcca ccatcaggac atcgggtagc
     1201 ccggcggtgc gcagcaggcg cactgcagct tcaatagatg catggtcgaa gtttgggccc
     1261 gtttggctgc ccccgcgaag cacgagatgg gcgtaaggat ttccagccgt ttccaggatc
     1321 gccggcgcgc cctccttggt cagcgacgga aacagatggc ggcttcgcgc cgcttggatc
     1381 gcgtcgacgg ctacctggac attgccgtct gtgcggttct tgattcccac cggcatagac
     1441 agacccgagg ccagctcccg atgcacttgg ctctccgctg tgcgggcgcc gatcgcaccc
     1501 cagctgatga ggtctgcata gaactggccg aaagtggtat cgagaaattc agtcccggcg
     1561 cagacgccga gttcgacaat gtcggcaagc aattgccgag cgaggcgcaa tcctttattg
     1621 atttgataag attcatttag gtcgggatcg ttgatcagtc ctttccagcc aacgacagtg
     1681 cgcggttttt cgaagtaaac gcgcatgaca atcaaaagcg cgctcgaaag cgcgtcgacg
     1741 atcggcttga gcttggtcgc gtattcgaga gccgctttgg tgtcgtgtac cgagcaaggc
     1801 ccgaccacga cgagaagccg gtgatccttg ccgtcaagaa tcgcctcaat cgcacggcgc
     1861 gatcggtaaa cgttttccga cgccgcttcg gtgagaggga tctcctcggc gaggatcgcc
     1921 ggtgacagca gtggccgagt tttgacgata cgaagatcgt cagttttcat tgttgctaga
     1981 cgtgtttgcg ctaggacgtc gaaacgctct gttcgctctc gacggttttc cgtaccgtcg
     2041 gccgattcat gatgcgctcc tgccatgcag tgtaggcgcc gagttccttc atggggaatc
     2101 cgccccgcaa cccccagcca tagaaaacca gcgcgtatcc gtcgacgacc gtgaagtcgc
     2161 ggcccatgat ccagtcgtgt ccctcgatca tcgaattgat ttcctggcaa ttggcccaga
     2221 atgattttcg gccgttttcc ttgacggcgg catgtgctgc ctcgctgtca gcgaaacgct
     2281 cgggccgcat gcagcgctga aatgagggat gaacgatgct cgagaaccaa cacatcgtgg
     2341 cgatgcaccg cgcctcctcg acaggatccg acggcatcag cttcttttct ggaaaccgcc
     2401 gcgctagata ggtgaggatt gcggtgttct caactaggat tttgccatcc gcatccaacg
     2461 agggcacttt gccacgcgga ttaattttga g
//
LOCUS       DASULZ010000599         3027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_678043, whole genome shotgun sequence.
ACCESSION   DASULZ010000599 DASULZ010000000
VERSION     DASULZ010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678043"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VFY96_12345"
     CDS             <1..741
                     /locus_tag="VFY96_12345"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX5607302.1"
                     /translation="ATVETVKNRVPLFIGVTSPNPREVVQKMNFVKDLGADGVLLGVP
                     YYEPLHVQDAIKFYHDIADLFPTLNIVVYHNPDNHKFTIPVAAFKELVKKSNIIGMKD
                     SHRTTQAFMNLQRIVAGKISVFVNQTQLYPYYAMGAAGCWSTEVWMGPWPIVYLLEQV
                     RKGDNQKAMEVIEDLGGVGAGKPVPGSGNKRPQEFGDYCKVGPTRVPFVTFPEAKLAE
                     AKGRAAHWMELNKKYRPLVEAMRSRPAA"
     gene            961..1404
                     /locus_tag="VFY96_12350"
     CDS             961..1404
                     /locus_tag="VFY96_12350"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HEX5607303.1"
                     /translation="MGFYAEPWLREARDKIRSVPEETGGQNPAGSDFDKLADAVEKAI
                     KAALIENHGSLPLQHDHSQLVSMCRSTGVWDILPPALRGLVEEIESYRSKKAAAVSDP
                     ISSGSADQSSAEQLQRYFFVARRLIDYMEFHVIGNDSVLKRLKVA"
     gene            1686..1970
                     /locus_tag="VFY96_12355"
     CDS             1686..1970
                     /locus_tag="VFY96_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607304.1"
                     /translation="MPGRRRSIMLASPGIGAVIAGLGIASFPRLSGRVHFVAMSVYAF
                     SISLVLFSFSRSLPLSMLLLILVGARNIAIRAVANTIVQMETPPELLGGC"
     gene            complement(2146..>3027)
                     /locus_tag="VFY96_12360"
     CDS             complement(2146..>3027)
                     /locus_tag="VFY96_12360"
                     /inference="COORDINATES: protein motif:HMM:NF027593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4921 family protein"
                     /protein_id="HEX5607305.1"
                     /translation="PFCEGNESMTPPEIYAQREARNDADTPGWRVRTVPNKYPALEDY
                     GRWSEADSSIDGTSPGVGVHEVIIETPRHIVNIGALDENQFVDFLRAYQARLGALRND
                     RRWRCLLIYKNQGEAAGATLEHVHSQLIALPFVPREVRDELTGVLRHYRSTGACFYCA
                     MSRTESASGNRLVALTERFVALCPFAPRFPYETWILPKHHSATFEGSSDEEILDLARA
                     LRNVIIKLNGALGNPAFNYFIHSLPPQESAEHQYHWHIEILPQVAKAAGLEWGSGIHL
                     NSVSPEDAARLLRKTPS"
BASE COUNT          660 a          797 c          866 g          704 t
ORIGIN      
        1 gcaacggtcg aaacagtaaa aaaccgggtg ccgctcttca tcggtgtcac cagccctaac
       61 ccgcgggaag ttgtccagaa gatgaacttc gtcaaggacc tcggggccga cggtgttctc
      121 cttggcgtgc cctattacga gccgctgcac gtgcaggacg ctatcaaatt ctaccatgac
      181 atcgccgacc tctttccgac gttgaatatc gtcgtctatc ataaccccga caatcacaag
      241 tttacgattc ctgtggccgc atttaaggag ttagtcaaaa agtccaacat catcgggatg
      301 aaagatagtc atcgcacgac ccaggcattc atgaacctgc aaaggatcgt ggctggtaaa
      361 atcagcgtat ttgtgaatca gacccaattg tatccgtact atgcgatggg cgcggcggga
      421 tgctggtcaa cggaagtttg gatggggccg tggccgattg tttacctact ggagcaggtg
      481 cgcaaaggtg acaatcaaaa ggcgatggaa gtgatagaag atctcggggg tgtcggcgcc
      541 ggtaagccgg ttcccggctc aggcaacaag cgcccgcaag agttcgggga ttattgcaaa
      601 gtcggcccta cgcgcgtgcc ttttgttacc tttccagaag ccaagctcgc ggaagcgaag
      661 ggccgcgccg cccactggat ggaactcaat aaaaaatacc gtcctctcgt ggaagcgatg
      721 cgctcacggc cagcagcctg atcaaaagag cggagcacgg tcgagacgaa agccctcggg
      781 aatccggggg ctttttgttt tgggccattg accggtggca tcaagctctg cagatgcaaa
      841 ttgtttcggc gatcgttctg ccgctgattg atgatcgggg cgcgcaacca tttgcttgcc
      901 ataaaactgc tataccctta tcatatttag caaaatcaat gtcaccggcg ggaggattgg
      961 atgggttttt acgctgagcc gtggctgcgt gaggcgcgag acaagattcg gtcggttcct
     1021 gaggagaccg gcgggcagaa ccccgccggc agtgatttcg acaaattagc tgacgccgtg
     1081 gaaaaggcga tcaaagcggc gcttatcgaa aaccacggta gcttacctct gcagcatgat
     1141 catagccaat tagtctcgat gtgccggtcc actggcgttt gggacatcct gccgccagct
     1201 ctgcgagggt tggtcgaaga gattgaatcg tatcgctcga aaaaagccgc ggccgtgagc
     1261 gatccgatat cgagcggatc ggctgaccaa tcttccgcgg aacaactgca aagatatttc
     1321 ttcgtcgcgc ggcgtctgat tgattacatg gaattccacg tcatcggcaa tgactcggtg
     1381 ctcaagcgct taaaagtggc atagcttccc acctccggcc gtccctctgg tgcggtcgac
     1441 catccgctcg gtaatctgtt cgtatttaca aaatcgcgat agttcgctca cgattcgtca
     1501 tgcaatttcc ggccgctcgc attaaactga acaatgagag ctcgttttgg caggaagtga
     1561 gcgagggata tcaatttctg cgcggacagc ggcggctctt tacgattatt tattgactta
     1621 cgacgtggcg ctagtgggca cgccgtattc gcgatttttg ccggtgtttg ccacggagat
     1681 cctgcatgcc gggccggcga cgttcgatta tgctcgcgtc gcccgggatc ggcgccgtaa
     1741 tagctggact tgggattgcg tcgtttcctc gtttaagcgg ccgggttcac tttgtcgcca
     1801 tgagtgttta cgcattttca atttcattgg tgctgttttc gttttcccgt tcccttccgc
     1861 tatcgatgct tctgctaata ctcgtcggtg ccaggaatat tgcgatccgc gccgtcgcga
     1921 atacgatcgt gcaaatggaa acgccgccgg aattgctggg cggatgctga gcctgttttt
     1981 catggacaaa gggctttgtc gttcggcacg ctgttcatcg ggagcgtggc gcatttcgcc
     2041 gggacgccga atgcaatagc gatagccggt gtatgctgcg cggtcgcggc atcgctgctg
     2101 ctttatcaac aaagccaaat gcgcgagagc cgagccgtgg aaccgctaac tgggcgtctt
     2161 gcgcaatagg cgagcagcgt cttcggggct cactgaattc aaatgaatgc ccgagcccca
     2221 ttccaaaccc gcggctttgg caacttgcgg caaaatttca atgtgccagt gatattgatg
     2281 ctcggcagat tcctgcggcg gaagtgagtg aatgaagtaa ttgaaggctg gatttccgag
     2341 agcgccgttg agtttaatga tcacgtttcg aagagcacgg gcgagatcta gaatctcttc
     2401 gtccgagctg ccctcgaaag tcgctgaatg atgttttggc aggatccacg tctcatacgg
     2461 gaagcgcggc gcaaacggac aaagcgccac gaagcgctcc gttaacgcaa ctaggcggtt
     2521 cccgctagcg gattccgttc ggctcatcgc acaataaaag caagcgcctg tggaccggta
     2581 gtgtcgaagg acgccagtga gttcgtcgcg cacttctcta ggaacgaacg gcagagcgat
     2641 cagctgcgaa tgaacatgct cgagggtcgc gccggcggct tcgccttgat ttttatagat
     2701 cagtaagcag cgccaacggc gatcgtttcg gagcgcccca aggcgcgcct ggtaagcgcg
     2761 caggaaatcc acgaattgat tttcatcgag cgcgccgatg ttgacgatat ggcgtggagt
     2821 ttcgatgatc acctcgtgaa caccaactcc cggcgaggtt ccatcaatgc ttgaatcggc
     2881 ttcgctccac ctgccgtaat cttcaagcgc tggatatttg tttggaactg tgcgcacgcg
     2941 ccaaccaggc gtgtctgcgt cattgcgcgc ttccctttgc gcatagattt ccggtggcgt
     3001 catgctttcg ttgccttcgc aaaacgg
//
LOCUS       DASULZ010000600         5576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1082683, whole genome shotgun sequence.
ACCESSION   DASULZ010000600 DASULZ010000000
VERSION     DASULZ010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5576
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082683"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VFY96_12365"
     CDS             complement(<1..533)
                     /locus_tag="VFY96_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607306.1"
                     /translation="MLIISTGSRLAPGFDLVDQQLLHAFRKITSPRIETYAENLDLIR
                     FPAEHFQRIFNEYLTAKYAGFPPDLVILVFVGNLRVPATMLPQLFPGTPIVVAGLTEE
                     EFHPDQFSRLVSGLAQRVDPRANLELILRVQPEIRRVVVIGGTAEVDRQVLQRIKKAG
                     DSFNERVAIDFWDNLSMA"
     gene            complement(789..1175)
                     /locus_tag="VFY96_12370"
     CDS             complement(789..1175)
                     /locus_tag="VFY96_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607307.1"
                     /translation="MNFQVKLFNWQDQGNHLIILGRGVLDSGAFRLLFDEIEAATQHL
                     GECKVLVDLSDSACQIESSEIQGLVARLPLGCWPRNNKVAFVSASDIADYHRLFFLRT
                     ELAARDLAVGVFRNSKLAIDWLAGMI"
     gene            1300..2340
                     /locus_tag="VFY96_12375"
     CDS             1300..2340
                     /locus_tag="VFY96_12375"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX5607308.1"
                     /translation="MISSSVTIGLIEALTDAGLAANKVLESLDLDPAVFSRADGFIPC
                     SSFARLLERAAELTGDFAFGLCFGARSNPKNIGALAYAVLNSVTVAAAFGTAARYLHV
                     HNEAAQVTLREANSVSYMTYSVQHLDVHESRQFTEYGIAIALNTLRVMVGSQWSPREV
                     HFAHEPPPTSVEHLQLFRAPVLFSCATNALVMDREFCQQPIPAADPTLFKVLSGYLEN
                     VLSHLPKEDPRLELIRRSIAEAIKHGNPSLGHVANALACSTRTLQRQLNTCATDFRTL
                     LDDTRKRLAEAYLKDSKNTLTQIAFLLGYSEVSTFNRSFKRWTGKTPLDYRRSLRAMI
                     LTEPSGPSLRQK"
     gene            complement(2395..2781)
                     /locus_tag="VFY96_12380"
     CDS             complement(2395..2781)
                     /locus_tag="VFY96_12380"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEX5607309.1"
                     /translation="MAARLLKMLGSGLRLRCPRCGIGKLYAKPFKMYDHCPNCNLKFE
                     REQGYFIGAMYINYGATVGVAVPGYLLLDAFTGMTINQQLALWVPFAVIFPLLFFHHA
                     RSLWLAFDHFFNPTQSLYSVPPKSFS"
     gene            complement(2783..>5576)
                     /gene="uvrA"
                     /locus_tag="VFY96_12385"
     CDS             complement(2783..>5576)
                     /gene="uvrA"
                     /locus_tag="VFY96_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX5607310.1"
                     /translation="KNISLNIPRGRFVVFTGLSGSGKSSLAFDIVYAEGQRRYIDSLS
                     AYARQFLEVMARPNVDYVAGIPPTVAIEQRLSQGGKKSTVATVTEIYHYLRLLYSKIG
                     KQHCVSCGRQIHSLTRSQILDRVGRSYRGKEVMILSPIVRGRKGFHKEVIAGARRLGY
                     RRARIDSKLVDLRTPELTNGLERFKEHNIDIIIGKAKAGGREVETLIDQGLRLGNGVI
                     HLLSERGEQIFNQRLFCLGCGIGYEPLDPRLFSFNSQQGACGQCSGMGFTWDFDPDLI
                     FADPQQPLRDALTGISPGSSLNGSEVERAMQRLLEQLENNCDVDRSFAKQPKKLQEEI
                     LYGGKGRGAFVGLVPYLKELCERSDQNSSSELDELMSEVPCTACQGRRLNSRAQAVKL
                     QGKTISEVTALSVEDAKVYFEALDLAGPGNAVRDQAVADKILREIQQRLNFLSEVGLP
                     YLTLDRRADTLSGGEAQRIRLAAQLGSNLRGVCYILDEPTIGLHPRDNQMLLGTLRRL
                     EKLGNSVLVVEHDEATIESADLIVDLGPGAGVRGGHVVSVGTVEEIKNNPASPTGAYL
                     RSAKKRLGPKRELSKAGWLTIRGAKAHNLKNIDVKVPLGMWTCVTGISGSGKSTLVRD
                     VIYKGLKLQLGQFAGRPGAHKEIAGGKALERVVEVDQTPIGKTPRSVPASYVGLLDEI
                     RKLYALAPEARLRGFTSSRFSFNVKGGRCETCAGQGKIRKEMSFLPDVFIDCDACSGA
                     RFNEETLAIRFNDKTIADVFHMTVEEAVPVFHAFPKVARPLKILEDIGMGYITLGQAS
                     NTLSGGEAQRIKLAYELGKESHGKTLYVLDEPTTGLHFADVDKLIEILHRLVDMGNTV
                     ITIEHNLDIIKDADYLIDLGPEGGAEGGKVVACGSPLEVSRDGAQSYTARFLREYLSN
                     GATQGASEQAHQQRIA"
BASE COUNT         1273 a         1562 c         1495 g         1246 t
ORIGIN      
        1 gccatgctta aattgtccca gaaatcgatc gcgacccgtt cgttgaagga gtctccagcc
       61 ttcttaatac gttggagcac ctgacgatcg acctccgccg tgccgccaat gaccacgaca
      121 cggcggatct cgggttgtac acgcagaatc aactccaggt tcgcgcgcgg atcgacacgc
      181 tgcgctaaac cgctgaccag ccggctaaac tgatcggggt gaaattcttc ttccgtgagg
      241 cctgcaacga caatgggcgt ccctggaaac agctgcggca gcatcgttgc agggactctc
      301 aagtttccaa caaaaaccag gattacaaga tcgggcggaa aacctgcgta tttcgcggtt
      361 aaatactcat tgaagattcg ctgaaaatgt tcggccggga atcgaatgag atcgagattc
      421 tctgcatagg tctcaatccg tggcgaggtg attttcctaa acgcgtgcag caactgctga
      481 tctacgaggt cgaatcccgg cgcgagtcgg cttcccgtcg aaatgatcaa cactcgcttc
      541 ggccgttccg tctccgcact gtagctatga ctcgacgcta agaagctggc aaacgcccac
      601 aggaggaaag caatccgacg tcggcgaagt tgaaaaaggc ggcccgtaca ccgaacaatc
      661 tgggaaagag ccacgacgtt cgtgacttta caaccaaccc tatactgaat caacttcagg
      721 aatgtgaaga cagacccgac ggaactccta ccgtgcgcag cactggcgcc accagtaatt
      781 ccacaacatt aaatcattcc ggcgagccaa tcgatggcga gttttgagtt tcgaaaaacg
      841 cccacggcca agtctcgtgc cgctagctcg gtgcgcagaa aaaagagccg atggtaatcg
      901 gcaatatccg acgccgagac gaatgcgact ttgttgttgc ggggccagca accaagcggc
      961 aatcgcgcga caagtccctg gatttcggag ctttcaattt gacacgcgct gtccgataag
     1021 tcgacaagca ccttgcactc gcctaagtgc tgcgtggctg cttcgatttc gtcaaacagc
     1081 aatctgaaag ctccactgtc cagaactcca cgacctagaa tgatcaagtg atttccctga
     1141 tcctgccaat taaataattt gacttggaag ttcatttttc cctcccaaat agctattaag
     1201 gatttcgagg agggatttct tgacattttg cgacaatggt ttgacagata acgctcgatc
     1261 gggccagagt ctttgaggca aattatgcac tcccgaccga tgatctcgtc aagcgtcacg
     1321 atcggcttaa tcgaagcctt gacggacgca ggcctcgctg ccaataaagt tttagagagt
     1381 ttggatttgg atcctgcagt tttctctcga gctgacggat ttattccttg ctccagtttt
     1441 gcgcgtctgc tggaaagggc cgcagaatta actggcgatt ttgcttttgg cttgtgtttt
     1501 ggcgcccgct cgaacccaaa aaatattggc gcgcttgcat acgcagtatt gaattcagtc
     1561 accgtcgccg ctgcttttgg aacggcggca cgttatctcc acgtccataa tgaagcggcg
     1621 caagtcacct tgcgtgaggc aaatagtgtc agttacatga cctacagcgt acagcatttg
     1681 gacgtgcacg aatcgaggca atttaccgag tacggcattg ctatcgctct caatacgcta
     1741 agagtcatgg tcggcagcca atggagtccg cgcgaggttc atttcgcgca tgagccgccc
     1801 ccaacaagtg tcgagcatct gcaactattc cgagcaccgg tgctattctc gtgtgccacc
     1861 aacgccctcg tgatggaccg cgagttctgc cagcagccga ttcccgccgc agacccaact
     1921 ctgtttaaag ttctcagtgg ctatttagag aacgtcctaa gccatctgcc gaaggaagat
     1981 ccaaggctcg agttgattcg tcgaagcatc gccgaggcga tcaaacatgg taatcccagc
     2041 ctcggtcatg tcgcaaacgc gttggcgtgc agcacgcgca cactacaaag acaattgaat
     2101 acctgcgcaa ctgacttcag gacgttgctc gatgataccc gcaagcgatt agcagaagcc
     2161 tatctgaaag actctaaaaa tacgctgaca caaatcgcgt ttttattggg ctattcggaa
     2221 gtaagtactt tcaatcgatc gtttaaacgg tggacgggta aaaccccttt ggactaccgc
     2281 cgcagtctcc gtgcaatgat tctcactgag ccctcgggtc cctcgctcag gcaaaaataa
     2341 tacgtggtgg gctttaccgt gaatgttgag taacgttaac cgaagaatga ccgcctagga
     2401 aaaacttttg ggcgggacgc tgtagaggct ctgagtagga ttgaagaagt gatcgaatgc
     2461 cagccagagg cttcgggcgt gatgaaagaa aagcaaagga aaaatcaccg caaaggggac
     2521 ccacagggcg agctgctgat tgatcgtcat gccggtgaac gcgtcgagga gaaggtatcc
     2581 aggcacggct acgccgacgg tggcgccgta gttgatgtac atggcgccga taaagtagcc
     2641 ctgctcgcgc tcgaatttca aattgcaatt cgggcaatga tcgtacattt tgaacggctt
     2701 cgcatataat ttgcctatcc cgcagcgcgg gcagcgcagc cgcaatccgg agccgagcat
     2761 ctttaacagt cgcgcagcca atttatgcga tccgctgttg gtgcgcctgt tcgctcgcgc
     2821 cctgggttgc gccgttactc agatattcgc gcagaaaacg cgcggtatac gactgcgcgc
     2881 cgtcacggga aacttccaga ggcgaaccgc aggcaacgac tttgccgccc tcggcgcccc
     2941 cttcgggacc gaggtcgatc aggtaatcgg catctttgat gatgtcgagg ttatgctcga
     3001 tcgtgatgac ggtgttgccc atatcgacga ggcgatgcag gatttcaatc agcttgtcga
     3061 cgtcggcgaa gtgtagtccg gtcgtcggct cgtcgagaac atacagtgtc ttcccatggg
     3121 attctttgcc gagctcataa gcgagcttga tgcgctgtgc ttcgccaccg gacagagtat
     3181 tgctcgcttg gccgagcgtg atgtagccca tgccgatatc ttcgaggatt ttaagcggcc
     3241 gggcgacctt cggaaacgca tgaaataccg gcactgcctc ctcgacggtc atgtggaaaa
     3301 catcggcgat ggttttgtcg ttgaagcgaa tggccaaggt ttcttcgttg aagcgtgcgc
     3361 cgctgcaggc gtcgcaatcg atgaaaacgt ccggcagaaa actcatttcc ttgcgaatct
     3421 tcccctgacc cgcgcaagtt tcgcaccgac cgcctttgac attgaacgaa aaacggctag
     3481 aggtgaaacc ccgcaaacgc gcctccggcg cgagcgcata gagcttgcga atctcatcaa
     3541 gcaggccgac atatgacgcg ggcacggacc gcggcgtttt accgatggga gtttgatcga
     3601 cttccaccac acgctcgagc gcttttcccc cagcgatttc tttatgcgcg ccgggccggc
     3661 cggcgaattg tccgagctgc agcttcaaac ctttgtaaat aacgtctcgc accaaagtgc
     3721 tcttgccgga acccgagatg cccgtgacgc aggtccacat tccgagcggt accttaacat
     3781 cgatattttt tagattgtgc gctttcgcgc cgcgaatcgt cagccagcca gccttactca
     3841 attcacgttt gggaccgagg cgtttttttg ccgagcggag ataagcgcct gtgggcgatg
     3901 cgggattgtt tttgatttct tcgaccgtgc cgaccgaaac cacatggccg ccgcgcacgc
     3961 cagcgccggg gccaagatcg acaatcaaat ctgccgactc gatggtcgcc tcatcatgtt
     4021 ccaccacaag gacgctgttg cccagcttct ctagccgccg cagagttccc agcaacatct
     4081 gattgtcacg cggatgtagt ccaatggtcg gctcatccag aatatagcag acaccgcgaa
     4141 ggttcgatcc aagctgagcg gcgagccgga tgcgctgcgc ctcgccgccc gaaagcgtgt
     4201 cggcgcgccg atcgagggtc aggtagggca aaccaacctc agacaagaaa ttcaaacgtt
     4261 gctgaatctc gcgaaggatt ttatccgcca ctgcttggtc gcggacggca ttccctggtc
     4321 cggccaaatc gagtgcttcg aaatagacct tggcgtcctc gactgaaagt gccgtcactt
     4381 ccgaaatggt tttgccctga agttttaccg cttgcgcacg cgagtttagg cgccggcctt
     4441 ggcatgccgt gcacggaact tcgctcatta actcatcgag ctcgctgctg gagttttggt
     4501 cgctccgttc gcacagctct ttgagatatg gcaccagtcc gacgaaagct ccgcgccctt
     4561 ttccgccgta aagaatttct tcctgaagct ttttcggttg tttggcaaat gacctatcga
     4621 catcgcaatt gttttccagt tgctcgagta gtcgctgcat cgcccgctcg acttcggagc
     4681 cgttcagcga cgatccaggc gagatacccg tcagagcatc ccggaggggt tgctgcggat
     4741 cggcaaaaat caggtccggg tcgaaatccc aggtaaaacc catgccgctg cattggccgc
     4801 acgccccttg ctggctgttg aacgaaaaca accgtgggtc gagcggctcg tagccgatgc
     4861 cgcaccccaa acagaaaagc cgctggttga aaatctgctc gccgcgctcg gacaagagat
     4921 gaatcacgcc attgccaagc cgtagtccct ggtcaatcag cgtctcgacc tcgcgcccgc
     4981 ccgccttggc tttgccgatg atgatgtcga tgttgtgttc tttgaaccgt tccaaaccgt
     5041 tggtcagctc cggggtgcgc aggtcgacca atttgctatc gattcgggcc cggcgatatc
     5101 cgagacgacg cgcgccagcg atcacttcct tgtgaaatcc tttgcgcccg cgcacgatcg
     5161 ggctcagaat catcacttct ttgccgcgat aggagcgtcc gacgcgatcg agaatctggc
     5221 tgcgcgtcag ggagtgaatc tggcggccgc aactgacgca atgctgcttg ccgattttcg
     5281 aatagagcag gcgcaggtaa tggtaaattt ccgtcaccgt cgcgacggtg gattttttac
     5341 cgccctggct gagtcgctgc tcgatggcta cggtcggcgg tatcccggcg acgtagtcga
     5401 cgttcggccg ggccatgact tcaagaaatt gccgcgcgta ggcagacaga ctatcgatgt
     5461 accgccgttg tccttcggca taaacaatat caaacgccag ggaagatttt cccgaaccgc
     5521 tgaggccggt gaaaaccaca aagcgcccgc gcggaatatt cagactgatg tttttt
//
LOCUS       DASULZ010000601         3339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_779079, whole genome shotgun sequence.
ACCESSION   DASULZ010000601 DASULZ010000000
VERSION     DASULZ010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779079"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VFY96_12390"
     CDS             complement(<1..628)
                     /locus_tag="VFY96_12390"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018988312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HEX5607311.1"
                     /translation="METLNVNLGERSYPIYVGAGILDSLGQRLQESGLVGKVAVVTNP
                     TVAQLYLDALHASLDDAGFEVIPILVPDGEEHKTFKSLTTIYDRLIAERCERKSSVLA
                     LGGGVIGDLAGFAAASYLRGVPYVQVPTTLLAQVDSSVGGKTGVNHENGKNLVGAFYQ
                     PKLVLIDVKVLASLPHRELVAGLAEVIKYGIIEDPQLFRDLEKNLTKIV"
     gene            complement(609..1172)
                     /locus_tag="VFY96_12395"
     CDS             complement(609..1172)
                     /locus_tag="VFY96_12395"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX5607312.1"
                     /translation="MTNAWSEKLQKNIALIGFMAVGKSAIGRTLARKLRRRFVDLDRV
                     IERAEGSKVREIFERKGEAYFRQLEKEALAGVLEGNNQVIATGGGVILDDQNIQILRE
                     KALLIGLSAEMDVLLARAGDGMKRPLLQGSNRREKIEDLLRQRASRYAQAHLTIDTSN
                     LTVDQVVKKIIGLIEVSKKTSDGNSKR"
     gene            complement(1370..2566)
                     /gene="aroC"
                     /locus_tag="VFY96_12400"
     CDS             complement(1370..2566)
                     /gene="aroC"
                     /locus_tag="VFY96_12400"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX5607313.1"
                     /translation="MLRYLTAGESHGPCLTMIIDGVPAGFPIQIDKINHDLWRRQQGY
                     GRGGRMLIEKDEAQVRSGIRWGETIGAPVALGIENRDWKNWTKKMSALPEDRDDKIAV
                     TKPRPGHADLNGALKYDRSDIRDILERASARETVARTAVGSFAKQLLAPFEIRIMGYI
                     RSIGSVAANLSQFSFKEIYARAEESPVRTADPQAEEKMIALIEECKKEGNTLGGIFEV
                     VVLGLPPGLGSHTQWDRKLDGRLAQALMSIQAMKGVEIGLGFEMARRRGSEVHDEIFF
                     DPSKDVSEGTPRIVPTGFYRGSNNSGGTEGGMTNGAPLVARVAMKPISTLMSPLQSVD
                     LRSKQPADASVERSDVCAAPAAAVVGESVVAFEVARAFLEKFGGDSLREVRRNYENYL
                     EQIKNY"
     gene            complement(2601..>3339)
                     /locus_tag="VFY96_12405"
     CDS             complement(2601..>3339)
                     /locus_tag="VFY96_12405"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HEX5607314.1"
                     /translation="LAGSPFAGAAAAAIPVLPIPSSGFRFGYFGNNINGVLSAAENEG
                     LVKIISRPSVVTLNNIPSTIKSERILRINLPSSTNIASGSGAAAGTAVATERIPVGIN
                     LTVIPQVSSDGFVLMNIKVKSSSLTTGFVSSGTAGVVPFDELNREAEANVLVKDGETI
                     VIGGILKDTDSTSTGGIPYLKDVPVLGWLFKNWRIQKDFEELVVFITPRIASSGSESI
                     PSAEQLWREQMKKTESSPVAVMQPGNP"
BASE COUNT          701 a          985 c          869 g          784 t
ORIGIN      
        1 cgacaatctt tgtcaggttt ttttccagat cccgaaaaag ctgcgggtcc tcgatgatgc
       61 catatttgat cacctcagcg aggcccgcaa ccagctctcg atggggcagc gaggcgagca
      121 cctttacgtc gatgaggaca agcttcggtt gataaaaagc gccgaccaga tttttgccgt
      181 tttcgtgatt gacgccggtt ttgccgccga cgctcgaatc cacctgcgcg agcaacgtcg
      241 ttggcacctg gacatagggc acgccacgga gataactggc tgcggcaaaa ccggcgagat
      301 ctccaatcac accgccgcca agcgcgagaa cagatgattt tcgctcgcag cgttcggcaa
      361 tgagccgatc gtagatcgtt gtcagcgatt tgaaggtttt gtgttcctcc ccatcgggca
      421 ccaggatcgg tatgacttcg aatccggcgt catcgagaga ggcatgcaag gcgtcgaggt
      481 acagttgcgc cacagtcggg ttagtgacaa cggcgacttt ccccactaat ccggattcct
      541 gaagccgttg gccgaggcta tcgaggattc cagcgccgac atagatcgga taagaacgct
      601 cgcccaaatt aacgtttaga gtttccatct gacgtttttt tgctgacctc gatcaagccg
      661 ataattttct ttaccacctg atccacggtc aaattgctgg tatcgatggt cagatgcgct
      721 tgggcatacc gcgaggcgcg ctggcgcagc agatcctcga ttttttcccg gcgattactt
      781 ccctgtaaaa gtggcctttt cattccatcg ccggctctcg caagcaacac atccatctcc
      841 gccgacaagc cgatcaggag cgccttttct cgcaatatct ggatattttg atcgtcgaga
      901 atgactccgc cgccggtggc gatgacctgg ttattcccct ccagcacccc cgccagcgcc
      961 tccttttcca gctggcgaaa gtacgcctcg cccttgcgct cgaaaatctc gcgcaccttg
     1021 ctgccttcgg ctctttcaat cactcgatcc agatcgacga agcgtcggcg cagctttctt
     1081 gccaaagtgc ggccaatcgc actcttgcca accgccatga acccgatgag cgcgatattt
     1141 ttctgcaatt tctcggacca ggcgtttgtc acttcagggc agctaccgcg ttagcaccgc
     1201 accgcttcca acggtggatg cctcacctcg gtcacagagg ctttcgcccg agggctgtca
     1261 tgctgagcgg ccagcgaagg atctcgcatt tcttcgatca cgaaaataag cccatgggca
     1321 ttgtcacaac gtgtggctgg gagacggtat cgttttcgcg cgagccggcc tagtagttct
     1381 tgatttgctc cagataattt tcgtaattac gccgaacctc gcgcaatgaa tcaccgccga
     1441 atttttccag aaatgcccga gcgacctcaa acgcaacgac ggactcgccg accaccgctg
     1501 cggccggcgc agcgcagaca tccgagcgct ccaccgaagc atcggcgggt tgctttgagc
     1561 gaagatctac cgattgtaaa ggactcatca aagtcgaaat tggcttcatc gccaccctgg
     1621 cgaccagcgg cgcgccgttg gtcatgccgc cttccgtccc tccggaatta ttgctgccgc
     1681 ggtaaaaacc cgtcgggacg atacgcggag tcccttcgct cacgtctttg ctggggtcga
     1741 aaaagatttc gtcgtgaacc tccgacccgc gtcgccgtgc catttcgaaa ccgagcccga
     1801 tctctacgcc tttcatcgcc tggatactca tcaatgcctg cgccaatcgt ccgtcgagct
     1861 tgcggtccca ttgggtatga gaacccaacc ccggtggcaa accgagcacc acaacttcga
     1921 aaataccgcc gagcgtattg ccctcttttt tacactcctc gatcaaagcg atcatctttt
     1981 cttcggcttg aggatcggca gttcggaccg gcgattcttc cgcacgagcg tagatctcct
     2041 tgaaggaaaa ctgacttagg ttggccgcga cactgccgat ggaacgaatg tagcccataa
     2101 tgcggatctc gaagggcgcc agcagttgtt tggcgaaaga tccgaccgcc gtgcgtgcca
     2161 cggtctcgcg cgcgctcgca cgttcgagaa tatcgcgaat atcggagcga tcgtatttaa
     2221 gtgcgccgtt gaggtcggca tgtccgggac gtggtttggt gacagcgatt ttgtcgtcgc
     2281 ggtcttctgg aagcgcggac attttctttg tccagttttt ccaatcacgg ttctcaatgc
     2341 ccagcgccac tggggcgccg atcgtttcgc cccagcgaat gcccgaacga acttgcgcct
     2401 cgtctttttc gatcaacatg cgcccgccgc gcccgtagcc ctgctgacgg cgccaaagat
     2461 catgattgat tttgtcgatt tgaataggaa agccagccgg cactccatcg ataatcatcg
     2521 tgaggcacgg cccgtgcgat tcgcccgcag tgaggtaacg caacatgcgg gcttatccta
     2581 gcttaataag atacagcagg ctaggggttc cccggctgca tgaccgcaac cggactgctc
     2641 tcggttttct tcatctgctc gcgccatagc tgttcggcgc tgggaatgct ttctgatcct
     2701 gacgaagcga ttctcggcgt gataaagacg acgagttctt cgaaatcttt ttgaatgcgc
     2761 cagtttttga acagccagcc gagcaccgga acgtccttta agtagggaat cccaccggtg
     2821 gaagtactat cagtatcttt gagaattccg ccgattacga tcgtttcgcc gtctttgacg
     2881 agcacatttg cctcggcctc acggttcagt tcatcgaagg gcacgactcc agccgtaccg
     2941 ctggagacaa agcctgtggt gagcgaactc gacttcactt taatgttcat taatacaaag
     3001 ccatcactgg atacttgcgg tataactgtc aggttgatac cgactgggat tctttcggtc
     3061 gccaccgcgg ttccggccgc ggcgccgctg ccactcgcaa tgtttgtcga actcggtaag
     3121 tttattcgca agattcgctc gcttttgatg gtcgacggaa tgttgttcaa agtcaccacc
     3181 gatggcctcg agataatctt gaccagccct tcgttttcag ccgcagagag tactccgtta
     3241 atgttgttgc caaagtatcc aaagcgaaag ccggaacttg gtatgggtaa aaccggtatc
     3301 gccgcagccg ccgcacccgc gaaaggactg ccggccagg
//
LOCUS       DASULZ010000602         4339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_779172, whole genome shotgun sequence.
ACCESSION   DASULZ010000602 DASULZ010000000
VERSION     DASULZ010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779172"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VFY96_12410"
     CDS             <1..344
                     /locus_tag="VFY96_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX5607315.1"
                     /translation="LYFAGYWGEAQGPEDPWIRALAEHLSRSDVKFYGASWCPHCADQ
                     KKLFDGSANSIPYVECSPGGPNAPQAEVCKDKNIQSYPTWIIDGQRYTGIQPLDALAQ
                     LSHFQYQGEKK"
     gene            341..667
                     /locus_tag="VFY96_12415"
     CDS             341..667
                     /locus_tag="VFY96_12415"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5607316.1"
                     /translation="MSSPRASWLLTFLTATLAMAIHARSGYADPAKYPEFAQQSLPDN
                     ITPVFIHIEELVAELKSGVKPVIIDVRTEEEFRELHILGAVSAPLSEFKSYLKSIPRD
                     RPVVLY"
     gene            816..1508
                     /gene="queC"
                     /locus_tag="VFY96_12420"
     CDS             816..1508
                     /gene="queC"
                     /locus_tag="VFY96_12420"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEX5607317.1"
                     /translation="MPAKKAVVLLSGGIDSATVAAIANQQGFELHALSFLYGQRHQRE
                     LESAKKVAAFLKLGSHRIIAFDLRSIGGSALTDSIDVPKRRTETEISHQIPITYVPAR
                     NTIFLAFALACAERIEAEAIFFGANQLDYSGYPDCREEYIQAFEQMANLATKAGVEGK
                     SRIKIHAPLLKMGKAQIIETGLNLGLDYGLTWSCYDPTSDGYACGQCDSCQIRLKGFR
                     EAGSKDPITYSG"
     gene            1950..2786
                     /locus_tag="VFY96_12425"
     CDS             1950..2786
                     /locus_tag="VFY96_12425"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5607318.1"
                     /translation="MRTRKEFLGSQTGSYSSPRRGTSLEFADYRRYAPGDDLRYLDWG
                     IYARSDRLYIKVFREEVDLFAYIFIDASGSMAFPSPQQKFLPATHVALALSYVILANQ
                     DHVKLHLLHDSSGRASPFYRGRRRMTDCIDFATSASPAGLLQLAPSLGEHLKRLRRPG
                     KAILISDFLMPAASYQQGFNLLRAFNLDISAIQILSRTEVDPPFSRGRLALVDSETQR
                     EINYEWSDDSRREYQSRLAHHNLEIRSFCHQSGIHYSLYVTDRELSDFIFATLPAIGL
                     FK"
     gene            2789..>4339
                     /locus_tag="VFY96_12430"
     CDS             2789..>4339
                     /locus_tag="VFY96_12430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HEX5607319.1"
                     /translation="MEFLNPTALLGLFALPLLLIPYLTKKRPRRLTFSSLILFTQSGT
                     PTSRGPWGRIHLPPLFFLQLLLITLLILALSEPVFSVRPTSIAIILDNSASMQALEDG
                     KTRLALAQQKAESLTQELGSGGRVDIYTTTPRLQRVNAEPLATVQANRVIAGIKAYDL
                     ADAPIDYDQSFGALAHEQNYQRIYLVTDHPAGSQGAIARVLSVGEAQPNLAITAFDVR
                     RSSIAATRLEASATISNFSSRNEKLRIALKGNGTTLTSRDVTVLAGKAATAVFEGFPG
                     HPTYEVEIEHSDALPLDNRRFAVAPASRNLRILTISPRPQAAASLKSIPGVTVDVVSP
                     ADYGKAVRSDYGLEIFHFAAPARLPDNPSLFIIPPEANPIVESGAPVGTVQVSSWREP
                     HELTRYINFSLFRPTYARPLKPKTAGDIIIESPTGPLAFALEHRASRYLFLGFDPLPY
                     LGRENLPMSIFTLNFLDWFLESAGTNAQGTAEPIAFGAVESGDTLITPAGKNIALNGR
                     ATVFQDTYE"
BASE COUNT          996 a         1254 c         1082 g         1007 t
ORIGIN      
        1 atctctactt cgccggctat tggggcgaag ctcaaggacc ggaggacccc tggattcgtg
       61 ctttagccga gcacttgtcc cggagcgatg tcaaattcta cggcgcgtcg tggtgcccac
      121 actgcgccga ccaaaaaaaa ctttttgacg gttcggctaa cagtattcct tacgtcgaat
      181 gcagtcccgg cggccccaat gcgcctcagg cggaagtctg caaagataag aacattcaaa
      241 gctaccccac gtggatcatc gatggacagc gctatacggg gatacaacca ttggacgcgc
      301 tggcgcaatt aagccatttt caatatcagg gggagaaaaa atgagttcgc cgcgcgctag
      361 ctggctttta acgtttttga cagcaacact cgcgatggcg atccacgcgc gctcgggcta
      421 cgcggacccg gcgaaatacc ccgagtttgc gcaacagagt ctgcccgaca acattacgcc
      481 ggtatttatt catatcgaag aattggttgc ggaattgaag tcgggcgtga agccggtgat
      541 catcgatgtg cgcaccgaag aggagtttcg cgagcttcat attctcggcg cagtgtcggc
      601 accgctcagc gagttcaaaa gttatttaaa aagtattcca cgcgaccgac cggtcgtgct
      661 ttattgagca tgtcctcatc acctggccag tctggcctat ggcatcctct acgaaaacgg
      721 ttatcggaac atgaaagttc tcgacgaggg tatcgtcggc tggtacgaaa agcgctatcc
      781 gatggaaggc accaaggcgc cgcgcccata gctttatgcc agccaaaaaa gctgtggttc
      841 tgctcagcgg cggcatcgac tcggccaccg tcgccgccat agcgaatcag caaggtttcg
      901 aattgcatgc gctgagtttt ctttatggcc aacggcatca aagagagctc gaatcggcca
      961 aaaaagtcgc tgcctttctt aagctcggct cgcatcgcat catcgccttt gatttgcgat
     1021 cgatcggtgg ctctgccctt accgacagca tcgacgttcc caagagaaga acggagacag
     1081 aaatctcgca ccaaatcccg atcacctacg tgccggcgcg gaatacgatc ttccttgctt
     1141 tcgcactggc gtgcgctgaa cgcatcgaag ctgaggcgat cttttttggc gccaaccagc
     1201 tcgactacag cggctacccc gactgccgtg aagaatatat tcaagccttc gaacaaatgg
     1261 cgaatctcgc caccaaagcc ggcgtggaag gcaaaagccg gataaaaatt cacgcgcctc
     1321 tgctgaaaat gggcaaagcg cagatcatcg aaaccggatt gaatctcggc ctcgattatg
     1381 gattgacatg gagctgttac gatccgacat ccgacggata cgcttgcggc cagtgcgaca
     1441 gctgtcaaat ccgtttgaaa ggattccgag aagcgggatc caaagatccc attacctaca
     1501 gcggctagtt tttcggaatg gacgcgcggc ttagccgatt catgcttgct cggggctgat
     1561 tcagttctca agccgagatc aatccgctat acattgtggc ttaaaatgcc acgatctatt
     1621 ctccacaagc cgaattttta cagctcagtc aaatgcgcca cgttctgcgg gtgacgagct
     1681 ggccgctatc taattttccg acgtttcgaa aagcacctcg aactcgtcaa atcctttcat
     1741 gaggtaatga cgcgggctat gtgacactcg ctgcaaaatc aaacgtttga aacagatacg
     1801 gacagatcgc gggttcctgc cggtccgttt ggtcttgcct ccgcttagtt tataatatct
     1861 ttcagtagtt ggcccactaa gcacaaatac atcctatgct aacggatccg tttacgcccc
     1921 agtttctgac ccagcttgaa gtccttcggt tgcggacacg aaaagaattt ctcggcagtc
     1981 agaccggcag ctactcatca ccgagacgcg gcaccagctt ggaatttgcc gattatcgcc
     2041 gatatgcacc tggcgacgat cttcgttatc tcgattgggg catttatgcg cggagcgatc
     2101 gtctctacat caaagtcttt cgcgaagaag tcgatctgtt cgcctacata ttcatagatg
     2161 caagtggttc aatggcgttt ccgtcgccgc agcagaaatt tctgcccgcg acgcacgtcg
     2221 cactggcatt atcctacgtc attctggcca accaggatca cgtaaagctg catctgttgc
     2281 acgattcgag cggccgagcc tcgccgtttt accgcggtcg gcgacgcatg acagattgta
     2341 tcgactttgc aacttcggcg agcccagcgg gtctgcttca acttgcgcct tctctcggcg
     2401 agcatctcaa gcgtctgcgc cggcctggca aggcaatcct gatttccgat ttcctcatgc
     2461 ccgccgcgtc ctatcaacag ggcttcaacc tgctgcgcgc tttcaatctc gacatttcgg
     2521 caattcagat tttatcgcgc accgaagtgg acccgccgtt ttctcgcgga cgcttggcct
     2581 tggtcgatag tgaaacgcaa cgagaaatca attacgaatg gagtgatgat tcgcgccgcg
     2641 agtatcagag ccggcttgcc catcacaatc tggaaatccg cagcttctgc catcagagtg
     2701 gaatccatta ctctttatac gtgaccgacc gtgagctgag cgactttatc ttcgcgacat
     2761 taccagctat cggcctgttc aagtagccat ggaatttcta aatcccactg ctttgctcgg
     2821 tttgttcgcg ctccccttac tgctgatccc gtatctgacc aaaaagcgcc cgcgccggct
     2881 gacattttcg agtttgatcc tctttacaca atcaggaacg ccaacgagcc gcggaccatg
     2941 gggacgcatc catttgccgc cgcttttctt cctccagttg ctgcttatca cattgttgat
     3001 tttggcgctg agcgagccgg ttttctccgt ccgtcccacc agcattgcca ttatactgga
     3061 caactcggcc agcatgcagg ctctcgaaga cggcaaaacg aggctggcgt tggcgcagca
     3121 aaaggcagaa agcctgactc aagaactcgg tagcggcggt cgagtggaca tctatacgac
     3181 cacgccgcga ctacagagag tgaatgccga accgttagcc acagtccaag ccaatcgagt
     3241 cattgccggt ataaaagctt atgatcttgc cgacgcgccc atcgattacg atcagagttt
     3301 tggcgctttg gcgcacgagc aaaattatca gcgcatctat ctcgttacgg atcatccggc
     3361 gggcagccaa ggcgctatag ctcgggtgct ttcggtcgga gaagcgcaac ccaacctcgc
     3421 catcaccgcc ttcgatgtgc gccgctcctc cattgccgcc acaaggctcg aagcaagcgc
     3481 cacaatcagc aatttctcca gtcgcaatga aaagctccga atagcgctta aaggcaacgg
     3541 gacaactctc acgagccgcg atgtcactgt cttagctgga aaagcggcca ccgcggtgtt
     3601 cgaaggtttt cccggccacc cgacctatga ggtcgaaatc gaacatagcg acgctttacc
     3661 tctggacaat cgacgctttg ctgttgcacc ggcttcgcga aatcttcgca ttctcaccat
     3721 ctcaccccgg ccacaagcag ccgcaagcct caaatcaatc cccggcgtca cggttgacgt
     3781 ggtttctccg gccgactacg gaaaagcggt tcgcagcgac tatggtttgg aaatattcca
     3841 tttcgccgcg cccgcaaggc taccagataa tccgagtctt tttattattc cacctgaagc
     3901 gaacccaatt gtcgaatccg gtgctcctgt cgggactgtg caagtatcca gctggcgcga
     3961 accccacgaa ttgacccgct atataaattt cagtctgttt cgtcccacgt acgcgcgccc
     4021 actgaagccg aaaaccgctg gagacatcat tattgagagt ccaaccggtc ctttggcctt
     4081 cgctctcgag caccgcgctt cgcgctatct gtttctcgga tttgatccct tgccttacct
     4141 gggccgcgag aatttgccga tgtctatttt tactctcaat tttcttgact ggttcttgga
     4201 gagcgcggga accaacgctc aaggtacggc cgagccgatc gcattcggcg ctgtggagtc
     4261 gggtgacact ctcatcaccc cggcgggaaa aaacattgcg ctaaacggtc gcgctactgt
     4321 cttccaagac acttacgag
//
LOCUS       DASULZ010000603         2126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1082907, whole genome shotgun sequence.
ACCESSION   DASULZ010000603 DASULZ010000000
VERSION     DASULZ010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082907"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VFY96_12435"
     CDS             <1..223
                     /locus_tag="VFY96_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607320.1"
                     /translation="EKNKIWVTCQTDDDLPYVLKYAGDDKLIIGADYGHNDTSSEILA
                     LRKLKEEGAVPTAVVNKILDDNARALYGL"
     gene            352..1377
                     /locus_tag="VFY96_12440"
     CDS             352..1377
                     /locus_tag="VFY96_12440"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5607321.1"
                     /translation="MIIDFQAHVFPEGYLAEMNRLDGAVILEEPDRHSGMQYFYDKKL
                     KCRINTATLQGQNIDERLKHMDELGIDIHVLTIPAPGADRFEASDAVKIARIANNAIA
                     AVARSHPKRFIGFFTLPSSDIKASLEELERSVNELGLRGFGCFANLNGQALDREELFP
                     IYERLAKYKLPVYIHPTAPLATEAVGIDIMPTLIFGWAFDSTVAMTRLVYGRVLERFP
                     EINWVVADVGGVLAFFAQRAINIYSGRTDEIRHKYGLKENPLDSFRRFYVDTADHPAS
                     TLRCVKDFFGADRMVLGTNYPYGPEQGRVLVKNSLKAIDGLGLEPGEKEKILGGNAAR
                     ILGIGAA"
     gene            1374..>2126
                     /locus_tag="VFY96_12445"
     CDS             1374..>2126
                     /locus_tag="VFY96_12445"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5607322.1"
                     /translation="MKFTKAARPVIMGANGVVSCGHPMAALAGVNILREGGNAVDAAL
                     AAAFVMSVVKPEASGPGGDLFALVYMKKTGRVEAINSSGPAPAKASIEYFGERGLKAI
                     PQSGALSIAVPGAVDGWLELHKKYATKELPRLMADAIRIARDGFPISQEFAENIEELS
                     SEYPWVDRCYRQPLGMVAPGKTLRQKGLSDVLEKIAQKGRDGFYSGEVADKICATVKA
                     EGGILTVEDLQPTVCEWLEPLSSTYRDGLVFEQ"
BASE COUNT          490 a          547 c          647 g          442 t
ORIGIN      
        1 cgagaaaaac aaaatctggg tgacctgcca gaccgacgac gatctgccat atgttctcaa
       61 atatgccggc gatgacaagc tcatcatcgg tgcagactac ggccataacg atacctcgtc
      121 ggaaattctt gccttgcgaa agttgaaaga agaaggggcg gtgccgacgg ccgtggtcaa
      181 taagattctc gacgataacg cgcgcgcttt gtacggccta tgagagaaaa atgtcgcagc
      241 ggcgaaattc gccagaatct ctcgttcatt gccacaatga aagcaagatc cttcggctag
      301 ccctcagggg gacggcaacg aattgtatct ttccggcaaa acaagaacga catgatcatt
      361 gattttcaag cgcacgtgtt tcctgaaggt tatcttgccg agatgaaccg gcttgatggc
      421 gcggtgattt tggaagagcc ggaccggcat agcggcatgc aatattttta cgacaagaag
      481 ctcaagtgcc ggatcaatac cgcgacgctg caggggcaga acatcgacga acgcttgaaa
      541 cacatggatg agcttgggat tgacatccat gtgctgacga ttccggctcc gggcgcggac
      601 cggttcgagg ccagcgacgc ggtaaaaatt gcgcgtatcg cgaacaatgc gattgccgcc
      661 gtcgcgcgca gccaccctaa acggttcatt ggttttttca cgctgccctc gagcgacatc
      721 aaagcatcgc ttgaggagtt ggagcgctcg gtgaacgaac tcggcctgcg cggcttcggc
      781 tgtttcgcga atttgaatgg ccaagctttg gatcgtgagg aactgtttcc aatttatgag
      841 cggctcgcaa aatataagct cccggtttac attcatccca ctgcgccgct cgcgacggaa
      901 gccgtgggca tcgacattat gccgacgttg atttttggct gggcattcga cagcaccgtg
      961 gccatgaccc gcctggttta tggtcgagta ttggagcgtt tccccgaaat caattgggtg
     1021 gtggcggatg tcggtggcgt gctggccttt ttcgcgcagc gcgcgatcaa catctatagc
     1081 ggacgcaccg atgagatccg tcataagtac gggctcaaag aaaatccgct ggattcgttt
     1141 cggcgcttct acgtcgacac cgccgatcat ccggcgtcga cgctgcgatg cgtgaaggat
     1201 tttttcggcg ccgatagaat ggttttggga acgaactatc cgtatgggcc ggaacaaggt
     1261 cgcgtgctgg tcaaaaacag tttaaaggcg attgacgggc tgggactcga gcccggcgaa
     1321 aaagaaaaga ttctcggcgg caatgcggcg cgtatcctag ggattggagc agcatgaagt
     1381 tcaccaaagc agcacgcccg gtcatcatgg gcgcgaatgg cgtggtctcg tgcggccatc
     1441 cgatggcggc actcgccggt gtcaatatac tacgtgaggg cgggaatgcc gtggacgcgg
     1501 ctctcgctgc ggcgtttgtg atgtcggtgg tcaagccgga agcgagcggt ccgggcggcg
     1561 atttgtttgc cttggtctat atgaaaaaaa ccggtagggt agaggcgatc aattctagcg
     1621 gaccagcgcc ggccaaggcg agcatcgaat atttcgggga acgggggctc aaggccattc
     1681 cccaatccgg agccttgagc atcgcggtgc ctggcgcggt cgatggctgg ttggagctgc
     1741 acaagaaata cgcgacaaaa gaactgccgc ggttgatggc ggatgcgatc cgcatcgcgc
     1801 gcgacggctt tccgatctcg caggagtttg ccgaaaacat cgaggagctc agctcggagt
     1861 atccctgggt cgatcgctgc tatcgccaac cgctcggcat ggtggcgccg gggaaaacgc
     1921 tcaggcagaa aggcttgagc gatgtactgg aaaaaatcgc acagaaaggg cgcgacggtt
     1981 tttactccgg cgaagttgcc gacaagatct gcgcaaccgt caaagccgaa ggcggcattc
     2041 tcacggttga agatctgcag ccgaccgttt gcgaatggct ggaaccgctc agctcaacct
     2101 atcgcgacgg gctcgtcttt gaacaa
//
LOCUS       DASULZ010000604         2333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1082934, whole genome shotgun sequence.
ACCESSION   DASULZ010000604 DASULZ010000000
VERSION     DASULZ010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082934"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(377..1084)
                     /locus_tag="VFY96_12450"
     CDS             complement(377..1084)
                     /locus_tag="VFY96_12450"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5607323.1"
                     /translation="MPSSWEKVKVGDSTMRLYMSARENQRPAGAVVVVQGQTGVDDFL
                     KFSDMVAREGFVAAAPDLYHRDAPDCKDDAPTRRMRLRDATVIQDINATVNFLKSHPS
                     VNSGRIGIVGFCMGGRVVYLMSAVNPDIKAGVMYYGSDPFSAWGEGASPFERTNDIHC
                     PIMGHFGEDDKNPSPADMKKLDAELTRLGKPHEFHSYPNAAHAFANFGSSNYREHAAS
                     DSWPRTFGFFAKHLKSK"
     gene            complement(1099..2244)
                     /locus_tag="VFY96_12455"
     CDS             complement(1099..2244)
                     /locus_tag="VFY96_12455"
                     /inference="COORDINATES: protein motif:HMM:NF006689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HEX5607324.1"
                     /translation="MSEAFDLLLTGGTVINPATKTNQKLDVGITDHRISAIGVSLPRT
                     DAKRVIDIEGSYVTPGLIDFHVHSYWGVNPYGCNLDALCLATGVTTTMDAGSAGPVNL
                     LGFRKLVYEASKTRMLGFVALAQHGVLNAPGELLNLDFADADGAAQAVSDNRDLEVGI
                     KLRLHKKAIGENSRKALRLAIKAGDATRTPIMVHVGDTAIGMDEIAATLRAGDIITHC
                     FTPQKPSIIDDKGNLLPEVRKAKERGVIFDVGHASGHFDFDLVERAMGQGLLPDIISS
                     DLHGRMTQPGFGIVGDLPTTLTKFLSLGLSFEQIITNCTINPARAVGWQDRIGSLEIG
                     CEADIAVLQVIDQPATLRDSVGKERLHKQRIAVKWTIRAGEVFEGRG"
BASE COUNT          527 a          656 c          611 g          539 t
ORIGIN      
        1 aaccagggaa gacaattgag accgatcggc gaaaacggga acggaacctt tttgacctgc
       61 gcgtgcagcg acgaatggaa gagcaaggcc gctattaaaa taaggagacc gatcgtaggt
      121 ttcggcaacc gataaaaaga cggaaaatca aacgagcatt tcatctcgga tgctcctctt
      181 aagaagattg cttgtctagt acagtcggct cattcgtgtc aatgcaaatc caggctgtct
      241 tactttcgat aacaatcggc tgtcatttcg agccccttcg actttgctca ggataactac
      301 gccgagaaat ctttccgaac gaatctatcc ggcaacggat tgaacgtttt gaccgggtca
      361 acggttttaa accgccttac ttactcttca gatgcttcgc gaaaaaccca aacgttcgcg
      421 gccaagaatc gctggccgcg tgctcgcggt aattgctcga gccaaagtta gcaaacgcgt
      481 gagcggcatt cggataggaa tggaattcgt gcggttttcc tagccgtgtc aattcggcat
      541 cgagcttttt catatccgcc ggtgacggat tcttatcgtc ctcgccgaag tgtcccatga
      601 tcgggcagtg aatgtcgttg gtgcgctcga agggtgacgc gccctcgccc catgccgaaa
      661 aagggtcact gccgtaatac atgacgccag ctttgatatc tggattcacg gccgacatta
      721 aatagacgac gcgtcctccc atacaaaatc cgacgatgcc tatgcggccg gaattgacgg
      781 aggggtgtga ctttagaaaa ttgacggtgg cattgatgtc ctggatcacg gttgcatcac
      841 gtaatcgcat tcgtcgagtc ggcgcatcat ctttgcagtc gggtgcgtcc cgatgataga
      901 ggtcgggcgc cgctgcgaca aaaccctcac gcgcgaccat atccgagaat ttcagaaaat
      961 catcgacgcc ggtttgtcct tgcacgacga caacggctcc cgcgggccgc tgattttccc
     1021 gcgcgctcat atagaggcgc atggtggagt caccgacttt taccttttcc catgatgacg
     1081 gcataaagcc tcccctcctt aacctcgccc ttcgaaaact tccccagcgc gaatcgtcca
     1141 cttcaccgcg atgcgctgct tgtgcaatcg ttccttaccc accgaatcgc gcagcgtggc
     1201 cggctggtcg atgacctgca aaaccgcaat atccgcttcg catccgattt ccaaactgcc
     1261 gatgcgatcc tgccaaccga ctgcccgagc aggattgatg gtgcagttcg tgatgatttg
     1321 ttcgaagctc aatccgaggg acaaaaattt ggtgagtgtc gtaggtaaat cgcccacgat
     1381 gccgaaaccc ggctgtgtca tccggccgtg aagatccgag ctgatgatat ccggcagcaa
     1441 accttgtccc atcgcccgct cgaccagatc gaagtcgaaa tgaccgctgg cgtgaccgac
     1501 gtcgaaaatc acgccgcgtt ctttggcctt gcgaacttcg gggagtagat tacctttgtc
     1561 gtcgataatg gacggcttct gcggcgtgaa acagtgcgtg atgatgtcgc cggcgcgcaa
     1621 cgttgcggcg atctcatcca tgccgatcgc cgtatcgccg acatggacca tgatcggcgt
     1681 acgcgtcgcg tcgccggctt tgatcgccaa acgcaacgcc ttgcggctat tctcacctat
     1741 cgctttcttg tgcagtctga gcttgatgcc gacttcaaga tcgcgattat cgctcaccgc
     1801 ctgcgcggct ccgtcggcat cggcgaaatc gagattcaat aattcgccgg gggcgttcag
     1861 cactccgtgc tgggcgagcg cgacgaagcc gagcatgcgc gtcttggaag cctcgtagac
     1921 cagtttgcgg aatccaagca aatttaccgg tccagcagaa ccggcgtcca tggtcgtcgt
     1981 cacgccggtg gcgaggcaaa gcgcatcgag attgcaacca tatggattga ctccccaata
     2041 actgtgcacg tgaaaatcga tcagccccgg cgtgacatag gagccctcga tatcgatcac
     2101 ccgctttgca tcggtgcgcg gcagactaac tccaattgcg ctaatccgat gatcggtgat
     2161 tccgacatcg agtttctgat tggtctttgt cgccggattg ataaccgttc caccggttag
     2221 caagagatca aaagcttcgc tcattggatc tgctgattag ttctcactcc caagtcattt
     2281 cttctccttg atgggatgaa ggaagggtga tcttgttcaa tggcaccccc cat
//
LOCUS       DASULZ010000605         2672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_779432, whole genome shotgun sequence.
ACCESSION   DASULZ010000605 DASULZ010000000
VERSION     DASULZ010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779432"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VFY96_12460"
     CDS             <1..291
                     /locus_tag="VFY96_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607325.1"
                     /translation="QAKNPLINGDVEKLWKLLGPRLVYCFLLAAGIALSILREQRLRG
                     TPAALSRTKGGVSRLRRIAGVWTFFALINFWNVTSAAFVAERGRLFLAVFGL"
     gene            313..2283
                     /locus_tag="VFY96_12465"
     CDS             313..2283
                     /locus_tag="VFY96_12465"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEX5607326.1"
                     /translation="MPRTTFSIAAVLAALLIFMGFRLKLYFERRAELRALALAEHLRI
                     FWVGLRLDAVIVSRTCAPIVLFALVLPDAYFVASRPFLLGYLGVLYFVCLFAEICGLY
                     FFCFYDCRLNYLVFEHGADREVVKTVLREYPVARIFFISLVGAAACLYLVAMTTSLGL
                     PQESAVAWRWDRAGSFFCLLVVAFATRGTFDHRPLNPSFASITTNRIANEIASCGVFN
                     LLTEWMNQLREVSTALQTLIKLPTAEEAVRLARERLSTHGSLTDDSSNPLVRQVNGRA
                     ASEPLNVVLVMMESFTGSLVGCLGGQPALSPELDKLASEGVLLKKCYATGERTIQALE
                     AAVSSFPPLPGVGVIKRPQARAGFSTLATVLKERDYETLFIYGGQGIFDEMRAFFMAN
                     GFDRFIEEKDFLNPTFRSTWGVSDEDLFHRADQEFSALDSRGRPFFATILTVSLHSPW
                     EYPPDRMQPLPVDFPVPAGFKLAELNNFLYADYAIGKFIRDARQAPYFDRTLFVFVGD
                     HGVHLRGADLVPIDEYRVPALFFCPSRLSPRQIAAVTSQIDLPPTIMAIIGGAYRSPF
                     FGRDVIHHCTNDPFAMAIYNKKRYGIVTERELIIMSENGERLSYERNRLDGGWRQAVF
                     STAQRRHADQAAALLRVAEDLLVSSRYTSIKG"
     gene            complement(2303..>2672)
                     /locus_tag="VFY96_12470"
     CDS             complement(2303..>2672)
                     /locus_tag="VFY96_12470"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5607327.1"
                     /translation="MVIDHFGHVGFDGGFERPVIRWVLDMLKNENWWMMVSDGNRDSK
                     MDASWDDAIPYGKAFVEAAPDRIVWGTDWPHPQWRKRMMNDAEEVELLYRYVDGDTGL
                     LRKILVKNPARLHGFEEQQQ"
BASE COUNT          485 a          764 c          763 g          660 t
ORIGIN      
        1 caggccaaga atccgctcat caacggtgac gtggaaaaat tatggaagct gctcggcccg
       61 cgtctcgtgt actgttttct gctggcggct ggcatcgcgc tttccattct gcgtgagcag
      121 cggctgcgcg gcaccccggc cgcgctgagc cgaaccaaag gcggtgtttc ccgtttgcgc
      181 agaatcgcgg gcgtttggac attctttgcg ctgatcaatt tctggaatgt gacctcggcg
      241 gctttcgttg cggaacgggg acgtttgttt cttgccgtct tcggcctgta aatccttgag
      301 ctggaactgt tcatgccacg aacaacattt tcgattgctg cggttttggc ggcgctgctg
      361 atattcatgg gcttccgtct caagctgtac ttcgagcgcc gcgcggaact gcgtgcgctg
      421 gcgttagccg agcatctgag gattttttgg gtgggcttac ggcttgacgc tgtgatcgtc
      481 agtcgaacct gtgcgcccat cgttcttttt gcgcttgttc tcccggatgc ctatttcgtc
      541 gcgagccgcc cgtttctgct gggttatctc ggcgtgttgt attttgtctg cctttttgcc
      601 gagatctgcg ggctttactt cttttgcttt tacgattgcc ggctcaacta tctggtcttc
      661 gaacatggcg ccgatagaga agtggtcaag acggttctca gggaatatcc ggtcgcacgc
      721 attttcttca tcagtttagt tggagccgcc gcttgtctct acctggtcgc catgacgact
      781 tcgctgggct tgccacaaga gagcgccgtc gcctggcgct gggatcgtgc cgggagtttt
      841 ttttgtctgc tggttgtggc gttcgccacg cgaggcactt tcgatcaccg accgttgaac
      901 cccagctttg cgtcgatcac gacaaaccgc atcgccaacg agatcgcctc gtgcggcgtt
      961 ttcaatctgc tcaccgaatg gatgaaccag ttgcgcgagg tatccaccgc gctccagacc
     1021 ttgatcaagc ttcctaccgc cgaggaggcg gtgcgtttgg cgcgtgaacg gttatcgacg
     1081 cacgggtcgc tcactgacga ttcgtccaac cccttggtcc ggcaggtcaa tgggcgggcg
     1141 gcgagcgaac cgctcaatgt cgtgttggtg atgatggaga gtttcaccgg cagcttggtg
     1201 ggttgtctcg gcggccagcc cgctctgtcg ccggagctgg acaaactggc aagtgaaggc
     1261 gtgctgctga agaaatgcta tgccaccggc gaacgaacca tccaggcatt ggaggcggct
     1321 gtgtcgtcgt ttccgccgtt gccgggcgtg ggcgtgatca agcggcctca ggcgcgcgct
     1381 ggattttcaa ccttggcgac ggtgctcaag gaacgcgact acgaaactct ttttatttat
     1441 ggcgggcagg gaatcttcga cgagatgcgc gcgtttttta tggccaacgg tttcgaccgt
     1501 tttatcgaag aaaaagattt tctcaatcct acttttcgca gcacctgggg ggtttccgac
     1561 gaagatctgt ttcaccgtgc ggaccaggag tttagcgctc tcgatagcag gggacgacca
     1621 ttttttgcga cgattctgac ggtgtcgttg cattcgcctt gggaatatcc gcccgatcga
     1681 atgcagccgc ttcccgtcga ttttccggtc cccgccggtt tcaaattggc ggaactcaac
     1741 aattttcttt atgccgacta cgccatcggc aaatttatcc gggacgcgcg ccaggcgccg
     1801 tatttcgacc ggacgctatt cgtcttcgtc ggtgaccacg gtgtccacct gcgcggcgcg
     1861 gatttggttc ccatcgacga gtacagggtt ccggcgttat tcttctgtcc atcccggttg
     1921 tcgccgcggc agatcgccgc agtaacaagc caaatcgatc taccgccgac gatcatggcc
     1981 attatcggtg gcgcttatcg gagtccgttc tttggccgcg acgttatcca tcactgcacc
     2041 aatgacccgt tcgccatggc gatctataac aaaaaacggt acgggatagt taccgaacga
     2101 gaattgatca tcatgtccga gaatggcgaa cgcttaagct acgaaagaaa ccggctggac
     2161 ggcggctggc ggcaagcggt gttttcgacc gcccagagac gacacgccga tcaagcggca
     2221 gcgctgcttc gggtggcgga ggatctgctc gtcagcagcc gctatacgag catcaaagga
     2281 taagagcctc ggcgcagcac cttcactgct gctgctcttc gaatccatgc agccttgcgg
     2341 ggttcttcac taagatcttg cgcagcaagc cggtgtctcc atcgacatag cggtagagca
     2401 actcgacttc ctccgcgtcg ttcatcatcc gcttgcgcca ctgcggatgc ggccaatcgg
     2461 tgccccagac aatgcggtcg ggcgccgcct cgacaaatgc cttgccgtag ggaatcgcat
     2521 cgtcccagct cgcatccatc tttgaatcgc gattgccgtc ggaaaccatc atccaccaat
     2581 tctcgttttt gagcatgtcg agtacccacc ggataactgg ccgttcgaaa ccgccgtcga
     2641 atcccacatg gccgaaatga tcgatgacca tc
//
LOCUS       DASULZ010000606         5451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_881683, whole genome shotgun sequence.
ACCESSION   DASULZ010000606 DASULZ010000000
VERSION     DASULZ010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5451
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881683"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..487)
                     /locus_tag="VFY96_12475"
     CDS             complement(122..487)
                     /locus_tag="VFY96_12475"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5607328.1"
                     /translation="MAKIRHIAIKVEDQEKTAEFYKQTFGMTEAWRGPVRDDGNRAIY
                     LTDGYINMAVLPARGGREGIDHFGFQVEDMESTLKTAQASGAKGEADKKPRDGRFAEM
                     GVHDPVGQLVDVTVHGWKS"
     gene            complement(509..1495)
                     /locus_tag="VFY96_12480"
     CDS             complement(509..1495)
                     /locus_tag="VFY96_12480"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607329.1"
                     /translation="MTNPNLFLLNAALLFCILVAVPVSAEKLTLAHVAINPSQGMLRV
                     ARDSGILAKYGFTADVILIPGTPRTIQALIAGDLDYVAAGAPASLRARAQGADVIALS
                     ALASFSSQRVFLRPDSKLTNMRDLKGKIIGVTQYGSGGDTFLRAALRKIGLKESEVTI
                     LQMGGTPGVAQGLESGRIEVGVLGDSGALLVFRGMAKYMKGGSAQEMGFRGIDAPITT
                     TERKLKSDRAAVVRFMQAYLETLHYFQTNKPGTARILSKYMRGVSEEHISLWCDDLRA
                     TVKPIPYIDEEALRSELEMMNAPTQQIPPGYVNNSILDEIKKSGFVEKLYRQ"
     gene            1759..2229
                     /locus_tag="VFY96_12485"
     CDS             1759..2229
                     /locus_tag="VFY96_12485"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5607330.1"
                     /translation="MSLHCKANEVPEYNRTQTGEAGTGQMVVRKGYGNECSLMIATRR
                     PGYHTKPHQHESEQINYVLDGEIWFFVKDKGYLCKKGDFHRIPANTVHWAYNRSDKDA
                     TVAEAHAPGLIGGRAGDSAAALFDEGEKPEIRGPGTNRFVPYDQERAEAAYFSK"
     gene            2234..3229
                     /locus_tag="VFY96_12490"
     CDS             2234..3229
                     /locus_tag="VFY96_12490"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607331.1"
                     /translation="MRIAEARSRRAGGLYIYTLVALTLLYSSAAAAQELRRIHYGTTT
                     STAHLPIWVAKDAGLFAKNGLNVEPIQIRGGALITMGIMSGQLQFSGAGAESAVAARV
                     EGGDIVLLACPVDSDSVYLIARPEIKSPAELKGKATAVTRLGSTTHFYLRAALRQVGI
                     DPEKDVTILQLGTGTETVSAMESGRISMAALTNRYAIPLLQRGWPMFVDLSTTDLVYP
                     SSCVASSRAFIKAEPKTVDDFLRAYVAAIHLIKKDQAFAEKTFAKWLREKDPYLIRKT
                     VESYAKLFKPAPYVPDKGIETVVKDLSNRRPVPKEFIGRPELFRDNAPLEKALSK"
     gene            3226..4293
                     /locus_tag="VFY96_12495"
     CDS             3226..4293
                     /locus_tag="VFY96_12495"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607332.1"
                     /translation="MMNLLTRRSRFRETATSLTFFARRLVKGKGEGTQSIFSLLLIAV
                     ALLWMHALAHTQESRRILYGVSTSISHLPVWVGKDTGLFAKNGLNIEPVQIRGGAVST
                     MAIMSGQAVLSGVGAGSVVAARVEGGDIVLLACPADLEPVYLIARPEIKSPAELKGKA
                     AGVTRLNSSIHFYLRSATRMVGIDSDKEMTLLQLGGSAEISAALEAGRIAAAALTIKD
                     VLPFTQRGWPVLADLTKSDLVYPSSCVTSTRAFVKAEPKVVNAFLNTYVSAIRLIKTD
                     QKLAERSLLKWLKEKESPTLSKSLEAFIRLFKPIPYVPDKGIENILKDLGLQRPVPNA
                     FIGHPELFRDNVPLERALPKS"
     gene            4322..5386
                     /locus_tag="VFY96_12500"
     CDS             4322..5386
                     /locus_tag="VFY96_12500"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607333.1"
                     /translation="MWSSAFNFIHPRPLAREGKGEGSSTNIALAVRIHILLCLILLWL
                     LFALSHAAAQELRRIFYGTTASPSHLPIWVAKDAGLFEKNGLNVEPVQIRGGSLITLA
                     IITGNLQFSGAGAESVVAARASGGDVVLLACPVNADPVYLITRPEIKSAQELKGQASA
                     VTRYGSTTHFYLRAALKHVGLNPEKDMTILQMGAGPEIVIALDRGVIAAAALTTRYAV
                     PFLRRGWPVLVDLSNTDLVYPSSCVTSSRAFIRAEPTTTHEFLRAYVAGIHLVKKNLA
                     FAEKSFAKWMREKDAGITKKTVESYARLFKTAPLVPDKGIENVIQDLVKARPDFKEYI
                     GWPEPFRENGPLQKVLKEKQ"
BASE COUNT         1216 a         1588 c         1432 g         1215 t
ORIGIN      
        1 aacgtcagga taaaaaggaa aaccagaaac gcgatccggc cgcgtttagt tgctgttttc
       61 ttcactgttg ccccttgtct taggcatggg gtgcctcgcc ttgtgcctga ttgcctggca
      121 tttacgattt ccacccatgc acggtgacat caaccagttg tccgaccgga tcgtgcacac
      181 ccatttcggc aaagcggcca tcgcgcggtt tcttatctgc ttcacccttg gcgccggacg
      241 cctgagcagt cttcaatgtc gattccatgt cttcgacttg aaacccgaag tgatcgattc
      301 cctcacggcc gccgcgcgct ggcaaaaccg ccatgttgat ataaccgtca gtgagataaa
      361 tcgcccggtt gccgtcgtcg cgcaccggac cacgccacgc ttcggtcata ccaaaggttt
      421 gtttgtaaaa ctccgcggtc ttctcctgat cttcgacttt gatcgcaata tgtctgattt
      481 ttgccatgat tctttcctcc tttaatgttt attgccggta aagtttttct acgaacccgc
      541 tttttttgat ttcatcgaga atactattgt tgacgtaacc cggcggaatt tgctgggtcg
      601 gcgcattcat catctcgagc tcggagcgca atgcctcttc gtcaatgtac ggaatcggct
      661 tgactgtcgc gcgtaagtcg tcacaccaaa gcgagatatg ctcctcgctc actccgcgca
      721 tatacttgct gagaatccgc gccgtgcccg gcttgttggt ttgaaaataa tgtagcgttt
      781 ccagatacgc ctgcatgaag cgcaccaccg ccgcgcggtc gcttttgagc ttgcgttctg
      841 tcgttgtgat cggtgcgtcg atgccgcgga agcccatctc ctgcgcgctt ccccccttca
      901 tgtatttggc cataccgcgg aaaactaaaa gcgcgcccga atcgccgagc acaccgactt
      961 cgatccggcc ggattcaagg ccttgcgcca cacccggtgt tccgcccatc tgtagaatgg
     1021 tgacttcgga ttccttcaag ccgatctttc tgagcgcggc gcgcaaaaag gtatcgcccc
     1081 cggagccgta ttgcgtgaca ccgatgatct tgcctttgag atctctcatg ttggtcaact
     1141 tggagtccgg ccgtaggaac acgcgttgag acgagaaact cgctaaggct gatagagcta
     1201 tcacatctgc gccctgcgcg cgtgccctga gcgatgccgg cgcgccggca gccacgtagt
     1261 ccaaatcgcc cgcgatcaat gcttgaatcg tgcgcggcgt gcctggaatc aagatcacat
     1321 ctgcggtaaa gccatacttt gccaggatac ccgagtctct ggcgacccga agcattcctt
     1381 gactcggatt gatggcaaca tgcgccaggg tcagtttctc agccgaaacg ggaacagcga
     1441 ccaaaatgca aaacagtaac gcagcgttca gtaaaaataa atttgggttt gtcacgcacc
     1501 ttcatttact gcccgaacgg taccgtgtca acggcgccga ccgttaatca ttttgtctca
     1561 tcgcgagtgt taacccattt ccgatttgtt atctctaacc cgttcgatac ctcagggtaa
     1621 actccatgag agatccttcc ccaattgaac gactaacgac gcgtgctcag tcccaccatc
     1681 ggtcacgatc cagcttgacg gaagggcctc tgtgtcatac gaaataggcg atttaaaatt
     1741 tctataggag gaaacgtcat gtccttacac tgcaaggcga acgaagttcc cgaatacaac
     1801 cgtacccaaa ccggggaagc cggaaccggt cagatggtcg tgcgtaaagg ctacggcaac
     1861 gaatgcagcc tcatgatcgc gaccagaagg cccggctatc ataccaaacc gcaccagcac
     1921 gaatccgaac agatcaatta tgttctcgat ggcgagatct ggttttttgt caaagataaa
     1981 ggttatctct gcaaaaaagg cgactttcat cgaataccgg ccaacacagt ccactgggca
     2041 tataaccgct ccgataaaga tgccacggtg gcagaggccc acgctcccgg gttgatagga
     2101 ggtcgcgccg gagatagcgc ggcagcgcta ttcgacgaag gcgaaaagcc ggaaatccgc
     2161 gggcccggca cgaaccggtt cgttccttat gaccaagaga gagcagaggc cgcgtacttc
     2221 agcaaataga gccatgcgga ttgctgaagc acgctctcgc cgcgccggcg gactctatat
     2281 ctatactctg gtggcgctga cactcctgta ttcgtccgcc gcagcggccc aggagctccg
     2341 tcggattcac tatggcacca cgacatccac cgcgcatttg ccgatctggg ttgccaaaga
     2401 tgctggcctt ttcgcgaaaa acggcttgaa cgtggagccg atccaaatcc gcggcggcgc
     2461 gctcatcacg atgggaatta tgagcggcca gctgcaattc tcgggcgccg gcgcagaatc
     2521 cgccgttgct gcgcgggtcg aaggcggcga tatcgtgctg ctcgcctgcc cggtggattc
     2581 cgattcggtc tatttaattg cgcgcccgga gatcaaatcg cccgccgagc tgaaaggtaa
     2641 agcaaccgcc gtcactcgcc tcggctccac aacgcatttt tatctgcgcg ccgcattgcg
     2701 gcaagttgga atcgacccgg aaaaggatgt gaccattttg cagcttggca ccggcaccga
     2761 aacggtctcg gcgatggaaa gcgggcgcat ttcgatggcg gcattgacga atcgctacgc
     2821 gattccgctg cttcagcgtg gctggccgat gttcgtcgac ttgagcacga cagatctggt
     2881 ttatccgtca tcctgcgtcg ccagcagtcg cgccttcatc aaagccgaac cgaaaaccgt
     2941 cgacgacttc ctgcgcgctt atgtcgcggc gattcacctg atcaagaaag accaagcatt
     3001 cgccgaaaaa actttcgcca aatggttacg ggagaaagat ccgtatttga tcaggaaaac
     3061 ggtcgaatca tatgccaaac tgttcaaacc ggcgccctac gtaccggaca aaggcatcga
     3121 aactgtcgtg aaagatctgt ccaatcggcg gccggtgccg aaggaattca tcggccggcc
     3181 ggaattattt cgcgacaacg caccactcga gaaagcgctt tcaaaatgat gaacctatta
     3241 accagacgtt ctcgtttccg tgagacggcg accagtctga catttttcgc gcgccggttg
     3301 gtgaagggaa agggcgaggg cacccaaagt atattttctc ttctcctcat cgcggttgcg
     3361 ctgctctgga tgcacgcgtt ggctcacacg caagagtcac ggcgtatcct ttacggcgtt
     3421 tccacttcga tttcccatct gccggtctgg gtcggcaagg acacggggct gttcgcgaag
     3481 aacggcttga acatcgagcc ggtgcaaatt cgcggcggcg cggtcagcac catggcgatt
     3541 atgagcggcc aggccgtcct ttccggcgtc ggggcgggct ctgtggtcgc agcccgcgtc
     3601 gaaggcggcg acattgtgct gctcgcatgc cctgccgatc tcgagccggt gtatctgatc
     3661 gcccgtcccg aaattaaatc ccccgccgag cttaaaggca aagccgcagg agttacgcgg
     3721 ctcaactcgt cgattcattt ctatctccgc tccgcaacca gaatggtggg tatcgattca
     3781 gataaagaaa tgaccctgct tcagttgggc ggcagcgccg aaatcagcgc cgcactcgaa
     3841 gcgggcagaa ttgctgcagc cgcattgaca ataaaggacg ttctgccgtt cacacagcgt
     3901 ggctggccgg tgctcgccga tttgactaag tccgatctcg tttatccctc atcttgcgtc
     3961 accagcacgc gcgcgttcgt caaagctgaa cccaaggttg tcaatgcctt tttgaacacc
     4021 tacgtaagcg caatccggct gatcaagaca gatcagaagt tggctgaacg atccttgcta
     4081 aaatggttga aggagaagga gtcgccgacc ttaagcaaat ctttggaggc tttcattcgt
     4141 ctcttcaagc cgattccgta tgtgcccgac aaaggcatcg agaatattct caaggatttg
     4201 ggacttcaac ggccggtgcc gaatgcattc atcggtcacc cggagctatt tcgggacaac
     4261 gtgcccttgg agcgagcctt gcccaaatca tgaacaagcg agtgcattag atcattgcac
     4321 aatgtggtct tccgccttca actttattca ccctcgcccg cttgcgagag agggcaaggg
     4381 tgagggctct tcgacgaaca ttgccctcgc agttcgaatt catatattgc tctgcctgat
     4441 tttactgtgg ctgctgttcg cgctgtcaca cgccgctgcc caagagttgc gccgaatttt
     4501 ttacggcacc acggcatcgc cgtctcatct gccaatctgg gtcgcaaaag acgccggctt
     4561 gtttgaaaaa aacgggctca acgtcgagcc cgtgcagatt cgcggcggct cgctgatcac
     4621 gctcgcgatc atcaccggca atttgcagtt ctcgggtgcc ggagcggaat cggtcgttgc
     4681 cgcgcgtgcc tctggtggcg acgtcgtctt gctcgcgtgt cctgtcaatg ccgatcccgt
     4741 ttatctaatc acgcggccgg aaatcaaatc cgcgcaggaa ttgaaaggcc aagccagtgc
     4801 ggtgacccgc tacggctcaa cgacccattt ctacttgcgc gccgcgctca agcacgtcgg
     4861 cctcaatccg gaaaaagata tgacgatctt acaaatgggc gccgggccag aaatcgtcat
     4921 tgccctcgat cgaggggtga tcgccgccgc cgcgctgacg acgcgctatg cggtgccgtt
     4981 cttgcgacga ggctggccgg tgctcgtcga tcttagcaac accgatctgg tctatccctc
     5041 ttcctgtgtg acaagcagcc gcgcttttat ccgcgccgag ccgacgacga cccacgagtt
     5101 tcttcgggcg tacgtcgccg gcatccacct cgtcaaaaag aatctcgctt tcgcggaaaa
     5161 atcctttgct aaatggatgc gcgaaaagga tgccggcatc accaagaaaa ccgtcgagtc
     5221 gtatgcgcgt ctattcaaaa ccgcgcctct cgttcccgac aaaggcattg aaaacgtcat
     5281 tcaagatctg gtcaaagcgc ggccggattt taaagaatat atcggctggc ccgagccctt
     5341 cagagagaac ggcccgctac aaaaagtcct caaagagaag caataacaat tagagtcgta
     5401 ggatgcggtg acgaaaggaa ccgcatcttc aacaccccat cactccacta c
//
LOCUS       DASULZ010000607         3443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_577785, whole genome shotgun sequence.
ACCESSION   DASULZ010000607 DASULZ010000000
VERSION     DASULZ010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577785"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1448
                     /locus_tag="VFY96_12505"
     CDS             <1..1448
                     /locus_tag="VFY96_12505"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5607334.1"
                     /translation="FYTITKHAAVIRMKTGVKNDGTLVARECEIHLDTGAYAEIGPRV
                     AKKSGYTAAGPYKIPHLKIDSYSVYTNKPPAGAFRGFGVSQSAWAVESQMDIIAAALH
                     MDPLEIRKKNGYEEGDKFVTEETLRAVGLKECLNQVAESIGWQSKGTSDSKSKKDSSI
                     GSARRGKGLACMIKATITPSISCAVVKLNEDASLSLYTGTVEMGQGSETVLAQIAGKE
                     LGIALEKIQVLGVDTDVVPYDLTTSSSRSTFHMGKAVQLAARHILRQLAAIAAVEYSV
                     PEQNITFADGKVRLPETQVDLAELMFKRFGMRGGTLVGEGQVKTSTKDEYGEKSTSAF
                     WFLAAGAAEVEVDIDTGKFKLLKYATAVDVGKALNPLGCRQQLAGAAITGIGQAMFEE
                     IAYDNGQLINPTLLDYILPSLGDMPATIDPICVEVPDPRGPFGAKGIGESALIPVAPA
                     IANAIYDAVGIRIKDLPIKAEKVYLGLHGIK"
     gene            1452..1889
                     /locus_tag="VFY96_12510"
     CDS             1452..1889
                     /locus_tag="VFY96_12510"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5607335.1"
                     /translation="MKSIGNGLFLSFVLIAAAACNSEVLERQAGQIREQEQEIARQRK
                     ELEALAAGQQVQDQKQQDCNRAFRDYFDKAQQSTNREQTIALYRDGLAICPDDDVAHY
                     ELGRALADAGRRADAEREYEAALKINPDFGDARRQLEAVRSKP"
     gene            1926..2699
                     /locus_tag="VFY96_12515"
     CDS             1926..2699
                     /locus_tag="VFY96_12515"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX5607336.1"
                     /translation="MTRLLGLAFSIFLGSLPAQAVDEKKLVDLTYSFNETTQHWPTAK
                     PFHLEKVSEGRTPAGYWYSSYEYSGSEHVGTHMDAPFHFAEGKWTTDQVPLSRTVGPG
                     VVIDVRPQTEKNREYLLRVEDVTGWEKMHGRIVAGSIVLARTGWGQYWGDRKRYFGTD
                     EEGNVTDLHFPGVSRDLAEFLVKQRSVKAVGIDTPSIDHGPSRDFPVHQILGAANVPI
                     FENVASMERLPAKGATIFALPMKIAGGSGAPLRIFAILP"
     gene            2810..>3443
                     /locus_tag="VFY96_12520"
     CDS             2810..>3443
                     /locus_tag="VFY96_12520"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX5607337.1"
                     /translation="MTEGTLSRKLRAIACIIGCWLGLAAMVSAAEIQMQVQAAPGQPS
                     ALSPTGKLLTIEDAVRIGLENHPRIRSAYERVGSQQAVLGQQMSSYYPTISMSNSYRT
                     AQASTSGGNDHAADAFASLTSFNFTLYNFGKREGNVQAARETLEATKQDYQTTNQDII
                     LAIKQAYYVYLGAQALVNVRKDTVRSRELLVQQARGFYEVGTRARIDVARA"
BASE COUNT          839 a          913 c         1000 g          691 t
ORIGIN      
        1 tattctacac catcaccaag cacgcggcgg tgattcgcat gaagaccggc gtcaaaaacg
       61 acggcacctt ggtggcgcgg gaatgcgaaa ttcatctcga cacgggcgcg tatgccgaaa
      121 tcggtccgcg ggtggcaaaa aaatcgggtt ataccgccgc gggaccgtac aaaattcccc
      181 acttgaaaat cgattcctat tccgtttaca cgaacaaacc tccggcaggg gcgtttcgtg
      241 gcttcggcgt gtcacaatcc gcatgggcag tagaatcgca gatggacatc attgccgctg
      301 ctctccacat ggatccgctc gaaatcagaa aaaagaacgg ttacgaggaa ggcgataaat
      361 tcgtgaccga agaaactctg cgggcggtcg ggctgaaaga gtgtctgaac caggtggcgg
      421 aatcgatcgg atggcaatcc aaggggacca gcgattcaaa aagcaaaaaa gattcatcga
      481 tcggcagtgc ccggcgcggt aaggggctcg cttgcatgat caaagcgacg atcacgccgt
      541 cgatttcttg cgctgtggta aagctcaacg aggatgccag cctatcctta tataccggca
      601 cagttgaaat gggccaggga tccgaaacgg tattggcgca aatcgccggg aaggagctcg
      661 ggattgcgtt agagaaaatc caagtgcttg gcgtcgacac tgacgtggtg ccgtacgatc
      721 taacgacatc ttccagtcgc tcgacttttc acatgggcaa agccgtgcaa ctggcggcgc
      781 ggcatatttt gcgacagttg gcggcgatcg ctgccgtgga gtatagcgtt ccggaacaaa
      841 acatcacgtt cgccgatggc aaggtccgcc tcccggaaac ccaagtcgat cttgccgagc
      901 tgatgttcaa acgttttggt atgcgcggcg gcactctcgt cggtgaagga caagtcaaaa
      961 cttcgacgaa agacgagtac ggcgagaaga gcacatcggc gttttggttt ctcgctgccg
     1021 gggcagccga ggttgaagtg gatatcgata cgggcaagtt caagctgctg aaatacgcaa
     1081 ctgcagtcga cgtcggcaaa gcgcttaatc cgctcggctg tcggcagcaa ctcgccgggg
     1141 ccgcgattac cggcatcggt caggcgatgt ttgaagaaat tgcctatgac aacgggcagt
     1201 tgatcaatcc gactctcctg gattacattt tgccttccct cggtgacatg ccggcgacaa
     1261 tcgatccgat ttgcgtcgag gtgccggatc caaggggtcc attcggagcc aaaggcatcg
     1321 gcgagagcgc gttgattccg gtggcgccgg cgatcgcaaa tgcgatctac gatgcggtcg
     1381 gcatacgcat taaagatcta ccgatcaagg ccgagaaagt ttatctcggc ttgcatggta
     1441 tcaaatagaa catgaaatcc atcggcaacg gacttttcct gagcttcgtt ctgatcgcag
     1501 cggcggcttg caactccgag gtgctagagc gacaagcggg gcagatccgt gagcaagaac
     1561 aagagatcgc gcgccagcgc aaagaattag aagcgctggc cgcggggcaa caagtccagg
     1621 accaaaaaca gcaagattgc aaccgcgcct ttcgcgacta ttttgataaa gcgcagcaat
     1681 cgacaaaccg ggagcagacg atcgctcttt accgcgatgg gttggcgatt tgcccggatg
     1741 atgatgtggc ccattatgaa ttgggccggg ccctcgccga tgcggggcgc cgcgctgacg
     1801 ctgaaagaga atacgaagcc gccctcaaga ttaatccgga tttcggtgat gcccggcgcc
     1861 aactcgaggc ggttcggtct aagccctagc gactaaccac actcacgcgt tcattggagc
     1921 atgacatgac acggctgctc ggtctcgcat tctcgatttt tctggggagc ttacccgcac
     1981 aagcagtcga cgagaaaaag cttgttgatc tcacttattc ctttaacgaa acaactcagc
     2041 actggcctac cgcgaagcct tttcatctgg aaaaagtcag cgagggcagg acaccggccg
     2101 gttactggta ttcgtcctat gagtacagtg gttccgagca cgtcggcact cacatggatg
     2161 cgccgtttca ttttgccgag ggaaagtgga cgaccgacca ggtgccgctt tcccggacag
     2221 tgggaccggg ggtggtaatc gatgtgcgcc ctcaaaccga aaaaaatcgc gagtacctgt
     2281 tacgagtcga agatgtgaca ggttgggaaa aaatgcatgg ccgaatagta gctggttcga
     2341 tcgtgctggc gcgcaccggc tggggacaat actggggtga tcgcaaacgc tacttcggca
     2401 ccgatgaaga ggggaacgtc actgaccttc acttcccggg agtttcgcgg gaccttgcgg
     2461 aatttctcgt caaacaaaga agcgtgaagg cggtgggaat cgacacgccg agcatcgatc
     2521 atgggccctc gcgagatttt cctgtccatc aaatcttggg agccgctaac gtgccgattt
     2581 tcgaaaacgt cgcctcaatg gagcgcttgc ccgccaaggg agcaaccatc ttcgcgctgc
     2641 ccatgaaaat tgccggcgga agcggcgcgc cgctcaggat ctttgcgatt ttgccataac
     2701 cgcgcaccaa cagacttggc ccggttcatg ctcgccgtct aacgtatttc cgtttttgct
     2761 ttcgaatcac aaaaacaata tattttgccg tcgcttaagg aggaaccgta tgaccgaggg
     2821 aacccttagc cggaaactgc gcgccatcgc ctgtatcatc ggatgctggc tcggtcttgc
     2881 ggcaatggtt tcggcggcgg aaatccagat gcaagtgcaa gcagcgccgg gtcaacccag
     2941 cgcgttatcg ccgacgggca aattgctaac catcgaggac gccgtgcgca tcggcctgga
     3001 aaatcatccc agaatacgat cggcatacga gcgggtagga tcgcagcagg cggtgctggg
     3061 gcagcagatg tcgtcgtact atccaacgat cagcatgagc aattcgtacc ggaccgccca
     3121 agccagcacc agtggcggca atgaccatgc agcggatgcc ttcgcgagcc tgacgagctt
     3181 caattttacg ctttacaatt tcggtaagcg cgaaggcaat gtgcaagcag cacgagaaac
     3241 actggaggcg actaaacagg attatcagac aacgaatcag gatatcattc tggcgatcaa
     3301 acaggcctat tatgtttact taggcgcgca ggctctcgtg aatgtgcgta aggacacggt
     3361 gcgcagtagg gaactcctgg tccagcaggc ccgaggcttt tacgaagtgg gcactcgcgc
     3421 aagaatcgat gtcgcccgag cgg
//
LOCUS       DASULZ010000608         2340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_983183, whole genome shotgun sequence.
ACCESSION   DASULZ010000608 DASULZ010000000
VERSION     DASULZ010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983183"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            338..1108
                     /locus_tag="VFY96_12525"
     CDS             338..1108
                     /locus_tag="VFY96_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015030074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5607338.1"
                     /translation="MIDGLKEKVVIVTGGGHGIGRAYCHGFAEAGAKVVIADIDAPAA
                     DKVAAEVIQQFDGKALGVKVDVANEMSTKEMAKIAVDKYGRIDILINNAAIFATIPMN
                     RGGIDSIDPAEWDRMMAVNLKGLFFCCRAVLPAMRQQKSGKIVNISSGTALNGSAGRI
                     HYVTSKAGVIGFTRTLAREVGDDNINVNAIAPGSTLSEDNPSEEILKMRGARVGDRCL
                     KRVQLPKDLVGTLLFLSSPLSDFMTGQTVSVDGGTAFL"
     gene            1345..2166
                     /locus_tag="VFY96_12530"
     CDS             1345..2166
                     /locus_tag="VFY96_12530"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5607339.1"
                     /translation="MTPYHDRIVVAGDIKTRFLEAGSGPDLVLLHGGEYGASAEITWR
                     ANIESLSKIFHVIAPDLLGWGLTDKLYSFSDPAGLRIKHLQRFLEALGIGKAFFVGNS
                     AGGGLILRASVRRPAPLQIEKMVTICGNASVFKTNSQADLENYTPSLENMRKIVALLY
                     HDPKWQTDENIRERFESSIIPGAWETLSAARLRSPVHQPRSTSHEFVSQLSQLKIPLL
                     IMSCEHDPLNQRDWDVNFQKIVPGSRVHRFMHSAHEPQIEESDEFNRVLTEFLLG"
BASE COUNT          544 a          629 c          632 g          535 t
ORIGIN      
        1 gggatgatcc ccttcagcca tcgcatctat cgtgtgttga caaaccaccg atctacggcc
       61 ttcagcgtgc cgatcccgaa agatcctgac ggccaataaa agtgacgctc ctgcacgcca
      121 cagccaagta gagctgctcc gagaacttgc ccccaagaag cgccgtccga ttctctctcc
      181 gtcattgttg aagatttgtt gaagatctct caccagacca acgcaaatcg gcgcagaagc
      241 gccggttgat tctgtgctgt tacctatggg aattactgag attgtatggg atcaaatccg
      301 agagtagcat aacggcaaat caaacgagga gtctaacatg attgatggac tgaaagaaaa
      361 agtagtgatc gtcaccggcg gcgggcacgg catcggtcgc gcgtattgcc acggttttgc
      421 cgaagctggc gctaaagtcg tgattgcaga tatcgatgcg cccgctgccg ataaagtagc
      481 agcggaagtg attcagcagt ttgatggtaa agccttgggg gtgaaggtcg acgtcgcaaa
      541 tgaaatgtcg accaaagaga tggccaagat tgcagtcgac aagtacgggc gcatcgacat
      601 tctcatcaac aacgccgcga tcttcgcgac cattcccatg aaccgcggcg gcatcgatag
      661 cattgatccc gcggaatggg atcgcatgat ggcggtgaat ttgaaaggct tgtttttttg
      721 ctgtcgcgca gtgctgccgg cgatgcgcca gcagaaatcc ggtaaaatcg tcaacatttc
      781 ctcaggcacg gcgctgaacg gcagtgccgg tcgtattcac tacgtaactt cgaaggccgg
      841 cgtgatcggc tttacccgaa cgctcgcgcg cgaagtcggc gatgacaaca tcaacgtcaa
      901 tgcaattgca cccggatcga cgctgtcaga agacaatccc tccgaggaga tcctcaaaat
      961 gcgcggcgct cgcgtcggcg accgctgcct gaagcgcgta cagcttccca aagatttggt
     1021 cggcacgctg cttttcctgt catcgccgct gagcgatttc atgacgggcc aaaccgtctc
     1081 ggtggacggt ggcaccgcgt ttctataaac ggctcggtag ataatgggct ctgctagtcc
     1141 ttacctgcca agctgtagag aatgttattt gcaaaatccc tttttgacaa cgagggaaaa
     1201 gatctgcagc atcgcggatt gctgcatcac aaaatctctc acctggtaaa acgggggtca
     1261 aggcggatca aagggatttc agcgccggag gtccgcactg ctcatgtaaa tagaactccg
     1321 cgaacctaac caggtctttc tcatatgact ccctatcacg atcgaattgt tgtcgctggc
     1381 gacatcaaaa cacgctttct ggaggccggc agcggaccgg atctcgtatt gctccacggg
     1441 ggggagtatg gtgcttctgc cgagatcacc tggcgcgcaa acatcgaaag tttgtcgaag
     1501 atttttcacg tgatcgcacc cgacttactc ggctggggat tgacggacaa gctctatagc
     1561 ttttccgatc ccgcgggctt gcgcattaaa catctgcaga gatttctcga agctttgggt
     1621 attggtaagg catttttcgt tggcaattcc gcgggcggcg ggctcatcct cagagcgtcc
     1681 gtgcgtcggc cggcgccgct gcagatcgaa aaaatggtaa cgatttgcgg caacgcgagc
     1741 gtgttcaaga ccaattcgca ggccgacttg gaaaactaca cgccgtcgct ggaaaacatg
     1801 cggaaaattg tcgctcttct ttatcacgat cctaagtggc agaccgacga aaacattcgc
     1861 gaacgctttg aaagctcgat catacccggc gcgtgggaaa ccttgtccgc ggcgcgcttg
     1921 cgaagtccgg tgcatcagcc gcgctcaacc tcgcatgagt ttgtttccca gttatctcaa
     1981 ttgaaaatcc cgcttttgat catgtcatgt gagcatgatc cgctgaacca gcgcgactgg
     2041 gacgtcaact tccagaaaat cgtgccgggt tcgagggtgc atcgcttcat gcactccgcc
     2101 catgagccgc aaatcgaaga gtccgacgag ttcaaccgag tgttgacgga atttctgctc
     2161 ggctaatgtc gtcagcaata gggctcggcc tgcttcgaca cttcgacttt gctcagtgcg
     2221 ggctccctcg actgggcctt cagtcccgag cttgtcgagg aatcagcatg cacggatttt
     2281 tgtcagatct tctcgttttt acgctcattc tgagcacgtc gaagattccg aaaggctttt
//
LOCUS       DASULZ010000609         2004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_578219, whole genome shotgun sequence.
ACCESSION   DASULZ010000609 DASULZ010000000
VERSION     DASULZ010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578219"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..157
                     /locus_tag="VFY96_12535"
     CDS             38..157
                     /locus_tag="VFY96_12535"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607340.1"
                     /translation="MSYGRNLAENFRRAAYYVDKILKGANPADLPSSNQQNSS"
     gene            237..1214
                     /locus_tag="VFY96_12540"
     CDS             237..1214
                     /locus_tag="VFY96_12540"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607341.1"
                     /translation="MKRLGIAASIVLLARLFLPGLAEAQPAPKVPRIGIVFIGGRNQP
                     HLASFKQGLRERGYIEGKNIHLEYRYAEGHYDRLPGLAADLVHEKVDVIVTTSSISAR
                     AARQATRTIPIVMASGSPVERGLAKSFSKPGGNVTGLSVFALELSGKRLELLKETVPK
                     MTRVAALWSSSENESVLGFKETQDAAKALSLRMQSVEVGDAGDMEKTFAALMKTPVDA
                     LLVVLSPLVTLNSRRIVELALKQRLPGMYPTRQFVEEGGLMGYGPLLGDLYRRAATYV
                     DKILKGAKPADLPVEQPTKFELVINLKTAKQIGLIIPPNVLARADRVIK"
     gene            1376..>2004
                     /locus_tag="VFY96_12545"
     CDS             1376..>2004
                     /locus_tag="VFY96_12545"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607342.1"
                     /translation="MRGLREIGYVEGQDILIDYRWAEGKLERLPVFIKELVQIKVNVI
                     VSSATPAIRAAKEQTSIIPIVMAGVTDPVGNGFVASLAHPGRNVTGLTHVAPDLTGKR
                     LELLKEVIPEVLRLGVLWNPNQPGQSAAFKEMQSAARAWKLTVISMEARNRKEMEKVF
                     SEAAKEHAEALLELPDPLLFVNRELIAATAAKLKLPTMYSFSEYVDAGGL"
BASE COUNT          471 a          525 c          586 g          422 t
ORIGIN      
        1 ctacgacacc acaacatttg tagaagctgg ggggctgatg tcctatggac gtaacctcgc
       61 agagaatttc cggcgcgctg catactacgt cgacaagatt ctcaagggcg cgaacccggc
      121 tgacttgccg tcgagcaacc aacaaaattc gagctagtaa tcaacctgaa aaccgccaag
      181 cagattggcc tcacgattcc gccgaatgtg ctggcgagag cggatagggt gatcaagtga
      241 aacgtctcgg aatcgccgcc agcattgtcc tgctcgcgcg cctgtttttg cccggcctgg
      301 cggaggcgca gcccgcgcct aaagttccgc gcattggtat cgtgttcata ggcggccgca
      361 atcagccgca cctcgcatcg ttcaagcaag gcctccgaga gcgcggttat attgaaggca
      421 aaaatattca cctggaatat cgctacgccg aagggcacta tgatcggctg cccggcctcg
      481 ccgcggatct cgtacatgaa aaagtcgatg tgatcgttac aacctcctcg atcagcgccc
      541 gagctgcgcg ccaggccacg cgcacgattc cgatcgtgat ggccagcggc agccccgtgg
      601 agcgaggatt ggcgaaaagc ttttcaaaac ctggcggaaa tgtcaccggc ctcagcgtgt
      661 tcgcattgga gttgagcggc aagcgtctcg aactcttgaa ggagacggtt ccgaaaatga
      721 cgcgcgtggc cgccctctgg agttcgagcg aaaacgaatc ggtgttaggc ttcaaagaga
      781 cgcaggacgc cgcaaaagcc ttgtcactgc ggatgcaatc cgttgaggta ggcgacgccg
      841 gcgacatgga aaagacgttc gccgcattga tgaagacacc ggttgatgcg cttctggtcg
      901 tgctgagccc gctcgtcacg cttaactccc gacgcatcgt cgagcttgcg ctcaagcagc
      961 gcctgcccgg tatgtatcca acgcggcaat ttgttgaaga gggcggcctc atgggctatg
     1021 gaccgctgct tggggatctt tatcgccgtg ccgcgacata cgtcgataaa atcttaaaag
     1081 gcgcaaaacc cgccgatctt ccggtcgagc aaccgacgaa gtttgagctg gtgatcaatc
     1141 tcaaaaccgc caagcagatc ggcctgataa ttccaccgaa tgtgctggcg agggcggatc
     1201 gggttattaa gtgatacacg gtaagggtga gagatcgaaa ctcattgtgt gtgcaatttg
     1261 cgttctcctg tttgtttttt gctcgtccgc acagggacaa cagcaaataa tccctcgaat
     1321 cgggtacgtg gatgcggggt cacctgcaac gactgggcac cgagccgacg cgtttgtgcg
     1381 gggattgcgg gaaattggtt atgttgaggg tcaagatatt ctaattgact accgttgggc
     1441 agagggaaag ttggaacgat tgcccgtatt cataaaagag ctggttcaga tcaaagtgaa
     1501 tgtgatcgtt tcatcggcta caccggcgat ccgagccgct aaagagcaga cttcgatcat
     1561 tccgatcgtt atggccggag ttaccgatcc tgtcggtaat ggttttgtgg ccagcctggc
     1621 gcacccagga agaaacgtaa cgggtctgac tcacgttgca cccgatctaa cgggaaaacg
     1681 gctggagtta cttaaggaag tgattccgga ggttttaagg ttgggcgtcc tgtggaatcc
     1741 aaatcaaccc ggccagtcgg ccgcttttaa ggagatgcag agcgcggcac gagcatggaa
     1801 actgactgta atttcgatgg aggcacgcaa ccgcaaagag atggagaagg ttttttcgga
     1861 ggcggcgaag gagcatgctg aggcattgct cgaacttccg gacccactgc tcttcgtcaa
     1921 ccgcgaactg atcgctgcaa ccgcggccaa actgaagctt cctacaatgt atagcttcag
     1981 tgaatacgtt gacgcgggcg gcct
//
LOCUS       DASULZ010000610         4785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_780135, whole genome shotgun sequence.
ACCESSION   DASULZ010000610 DASULZ010000000
VERSION     DASULZ010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780135"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..933
                     /locus_tag="VFY96_12550"
     CDS             187..933
                     /locus_tag="VFY96_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX5607343.1"
                     /translation="MTEGMSLNERADALERKMSDELDVLISKSALFNMADGKLGAVNM
                     PSKGGKYQLMDDDPRLPKMPAKPTLLDYFTYRASGINHMLQSATHALKAGMPEKTILA
                     CLLHDISNAIFIKSDHGYWGAQLIEPYVDEEVSWAVRAHQALRFFPDESVGYAYPEMY
                     VNHFGRNYEPDGYIKEAYERARDHKWYMTARMITVHDIYSFDPNAVVSWESFVDIVGR
                     NFKQPDEGLGFDNSPVAHMWRTIRRPTKYL"
     gene            995..1555
                     /locus_tag="VFY96_12555"
     CDS             995..1555
                     /locus_tag="VFY96_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607344.1"
                     /translation="MFESDRFIDDCRQCLGEQNPHAAVREIVARAVSEPGQVLKTLGE
                     PRLSGIQTLYRSDTLTILNVLWGPGMTLYPHEHRMWAVIGIYSGREENSFYQRSENGL
                     RAHAAKTLDLKETLPLGEAVIHAVTNPLDRITAAIHVYGGDFFAVPRSEWDPQTLQER
                     PFDIERAKEVFAEANRRLVSADTVSR"
     gene            1819..2211
                     /locus_tag="VFY96_12560"
     CDS             1819..2211
                     /locus_tag="VFY96_12560"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5607345.1"
                     /translation="MGKTAFNPDGLPAPRGSYSLVNIAQPGRMVFIAGQTASDHDGKV
                     VGVGDAKAQTRFILGKIQRAVEAARGTMNDVVALNVFTTDVRNHRDINETRREVLGTN
                     FPTSTMVQVVALARPELLLEINAIAVIP"
     gene            complement(2364..2969)
                     /locus_tag="VFY96_12565"
     CDS             complement(2364..2969)
                     /locus_tag="VFY96_12565"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF015875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HEX5607346.1"
                     /translation="MSLAGLGKAKQRCSRCGASLTLCMCAEVPRLGLRTKISLVIHHR
                     ELSRSSNTGLLAHQSLVNSEVRIRGESRETLDLSDLLSPDYRTLLFFPSTDALELDRE
                     LVCQDSRPIQLIVPDGTWRQAHKVHSRHPELRNIPRVKISAPNHAIFQLRAQSRPERM
                     ATLQAIACALRVIEGGLAAARLMKLYEARIDRTLRARGVRI"
     gene            3171..3761
                     /locus_tag="VFY96_12570"
     CDS             3171..3761
                     /locus_tag="VFY96_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607347.1"
                     /translation="MQAFDTVSIDELVALPPTIFGVIRWKQYIGRPAGHLCSGFNIRV
                     EEHTATQFRAVGGGKYEPIPGTGIWKDVVDSLVCQELPDEGDYHKLGFQIRGRDIHLN
                     AFPDGQYRITPTLTGMWTRATPVVLLGSRIIEPTSAYVVLKKDDHIQSVEFEVVQRPR
                     PWATILGAAIAGALVGSALRRANPTSSNLVRSGPGD"
     gene            3758..>4785
                     /locus_tag="VFY96_12575"
     CDS             3758..>4785
                     /locus_tag="VFY96_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HEX5607348.1"
                     /translation="MSRFSHFQMHEEHRPPDCTVRFHTTSLLALIVGYAACAAVGCST
                     TSWKSPLGRDHPLTGRIWDVSSARFIDRETLVTRLARADFVLLGERHDNLDHHQLQAE
                     VLRSLIAGGRRPAVGFEMLGLDDAGAVAKHLAAAPNDAAGLGQVVNWNQRGWPDWAMY
                     QPIADAALQANLRIVATNLPLSTATKMTRNGVDALEPRMVRELGLDRPPPEPVFASMA
                     ADIRDAHCGYAPEEIVKPMVDVQRARDAQMAESLIAAADADGAVLVAGAGHVRNDYGI
                     PVHLRAKAPKKQVISIAFVEVDKQQTAPQSYALPYPNDRLPFDYVWFTPRVDAEDPCE
                     KFKSHFDR"
BASE COUNT         1055 a         1371 c         1373 g          986 t
ORIGIN      
        1 cgaaatggtt gagatcgtga tgtctaaccc aggctatctg acgagcggac acccccggac
       61 gtgcagttga tggtgaattt cacccgccac tccggaccca agacggccaa gccgaaatca
      121 ttgacacatg ccggcgcggt gatacgatcc ggcgcggtga gctcaagaac atgaaggagg
      181 cgaatcatga ctgaaggtat gtcactgaat gagcgcgcgg acgccctcga gcgaaagatg
      241 agcgacgagc tcgacgtgct gatctcgaaa tcggcattgt tcaatatggc ggacggaaaa
      301 ctcggggcgg tcaacatgcc ttccaaaggc ggcaagtatc aattgatgga cgacgatcca
      361 cggctgccga aaatgccggc caagccaacg ctcctggact atttcacata ccgcgccagc
      421 ggcatcaacc atatgctgca aagcgccacc cacgcgctga aggcgggcat gccggagaaa
      481 acgattctcg cttgccttct ccacgatatt tccaacgcga tcttcatcaa aagcgaccac
      541 ggctactggg gcgcgcagtt gatcgagccc tacgtggacg aggaagtctc gtgggcggtg
      601 cgcgcgcacc aggcgctgcg cttcttcccc gacgaatcgg tcgggtatgc ctatccggaa
      661 atgtacgtca accatttcgg gcgaaactac gagcccgacg gttacatcaa agaggcgtac
      721 gagcgcgccc gcgatcacaa gtggtatatg accgcgcgca tgattacggt tcacgacatc
      781 tattccttcg accccaacgc ggtggtgagc tgggaatcct tcgtcgatat tgtcgggcgt
      841 aacttcaagc agccagacga gggtttgggc ttcgacaata gccccgtcgc gcacatgtgg
      901 cgcaccatcc gccgtccgac caagtatctt taatcgattt tggattttag attagggatt
      961 tcggatagcg attagaatcc gggaaaggcg tgtcatgttc gaatcggatc gatttattga
     1021 tgattgtcgc cagtgccttg gagagcaaaa tccgcacgcc gcggtgcgtg aaatcgtcgc
     1081 cagggcggtg agtgagccgg gccaggttct caaaacgctg ggagagccga gactctcagg
     1141 cattcagacg ctctaccgct ctgacacatt gacgatttta aacgttctgt ggggacccgg
     1201 gatgactctg tacccgcatg agcatcgaat gtgggcggtg atcggaattt acagcggacg
     1261 ggaggaaaac tcgttttatc agaggagcga aaacggtctc cgagcgcacg ccgcaaagac
     1321 gctcgacctc aaagaaactc ttccgctcgg cgaagcagtc atccacgcag tgactaatcc
     1381 gctcgaccga atcaccgcgg cgatccacgt ctacggcggc gacttcttcg ccgtgccgcg
     1441 cagcgaatgg gatccgcaaa cgctgcaaga gcggcccttc gacatcgagc gcgcgaaaga
     1501 agtatttgca gaagcgaaca ggcgcttagt ttccgccgac acggtttcgc gttaaatgcc
     1561 aggaggagac tcgatcccca cgaacgagaa atctacgtcc ttatgcgact caactacgat
     1621 gtcgcagcgc atcggggcaa tatttgttgt tacatcatgg cttccgccgc ggtttcaatc
     1681 taaagataac gatttgttct ggtccagtgc gtctgccttt gaggagacgc gattccgccc
     1741 aaggacaagt aaagcgttcg actgtgagcc gaagccggcc ggttcattca ctactttatt
     1801 tcataggagg aaactcagat gggcaagacc gcattcaatc ccgatggact gccggcgccg
     1861 cgcggaagtt attcgctggt caacatcgca cagccgggac ggatggtgtt catcgccggc
     1921 cagaccgctt ccgatcacga cggcaaggtt gtcggcgtcg gcgacgccaa ggcgcagaca
     1981 cgctttattc tcggaaagat tcaacgcgcc gtcgaagcgg cccgcgggac gatgaacgat
     2041 gtcgtcgcgc tcaatgtctt caccaccgac gtgcgcaatc atcgcgacat caacgaaacc
     2101 cggcgcgaag tcctgggcac aaattttccg accagcacga tggtgcaggt ggtggcgctg
     2161 gcgcgtcccg agctgctgct cgaaatcaac gcgattgcgg ttattccgta aggaaagcga
     2221 ctttgatttt tttaaaactg acgcgggagg agcaaagtca tcatccgcac tttgcaaaag
     2281 gcgggttgag ggaggtttga ttccgcggca cgttggaaaa cctatgtcgc ccctgcgtta
     2341 cgatcgactc aggccatgct ttctcaaatt cgaacgccgc gcgctctgag ggttcgatcg
     2401 attctggcct cgtagagttt catcaatctg gctgccgcga gaccgccttc gatcactcga
     2461 agcgcgcacg cgatcgcctg caaggttgcc attctttccg gccggctttg cgcgcggagt
     2521 tgaaagattg cgtgattcgg tgcgctaatc ttcactcgcg gaatgtttct tagctcgggg
     2581 tggcgcgagt gaactttgtg agcctggcgc caggtgccgt ccggcacgat cagttgaatc
     2641 ggccgcgaat cctggcagac cagttcccga tcgagttcca gcgcatcggt gctcggaaaa
     2701 aacagcaacg tccgataatc aggcgacaac agatcgctca gatcgagcgt ctcccggctt
     2761 tccccgcgaa tgcgcacctc gctgttgacc agagattgat gcgcgagcag accggtgtta
     2821 ctgctccgcg agagctctcg gtgatgaatc accagtgaaa tcttggtgcg cagcccgagg
     2881 cgcggcacct ccgcgcacat gcacagcgtg agcgatgcgc cgcaccgcga gcagcgttgt
     2941 tttgcctttc ccaaaccagc caacgacaag agtcctcgac cgctatatat acttaccgac
     3001 cacgcgatgc catcggttat tgatcgcata ccgcatctga tttctcgccg tgattccggt
     3061 gcaaaccgga atcaaaattt tttctgaaca ggtcttgcga attccgtgca gcggcctacg
     3121 ctggctatag ttcggcctga gactcgagaa ttctatcgca ggagaaaata atgcaagcgt
     3181 ttgacacagt ttcgattgac gaactcgtgg cgttaccacc gacgatcttt ggagtcatcc
     3241 gttggaagca atacatcggc aggcctgcgg gtcatctgtg cagcggtttc aatatccgtg
     3301 tggaagagca tacggcaacc cagttccgcg ctgtcggcgg tggaaaatac gagccgattc
     3361 ccggcaccgg catctggaaa gatgtcgtcg attcgcttgt ttgccaggag ctacccgatg
     3421 aaggcgatta tcacaagctt ggttttcaaa tccggggccg ggatatccat ctgaatgctt
     3481 ttcccgatgg gcagtatcga atcacaccga ctctgaccgg catgtggacc cgggccactc
     3541 cggtcgtgtt gctcggctcg cgcattatcg agccaacgag cgcgtatgtt gtgcttaaga
     3601 aggatgatca tattcaaagc gtcgagttcg aggtcgtgca acgtccccgg ccatgggcca
     3661 caatcctcgg cgcagccatc gcaggcgcgt tggtgggctc tgcgctacga agagcaaatc
     3721 cgacttcgtc taacctggtc agatcaggac cgggagattg agccgcttct cgcactttca
     3781 gatgcacgag gaacatcggc caccggattg tacagtaaga tttcacacga cgagtctact
     3841 tgcactgatc gtcggctacg cagcgtgtgc cgcggtggga tgttcgacca cgtcgtggaa
     3901 atcaccgcta ggtcgagatc atccactcac cggccggatc tgggacgtct cctcggcccg
     3961 attcatcgat cgcgagactc tggttacccg tttggcgcgc gctgatttcg tcctgctcgg
     4021 cgagcggcac gataacctcg accatcatca gctgcaggcc gaagtgcttc ggagcctgat
     4081 tgcgggcggg cggcgtccgg cagtcggttt tgaaatgctc ggcctcgacg atgccggcgc
     4141 tgtcgccaag catctcgccg ccgctccgaa cgatgctgcg gggctaggtc aggtagtgaa
     4201 ttggaaccag cgtggctggc cggattgggc gatgtatcaa ccgatcgccg acgccgccct
     4261 tcaagcaaat cttcggatcg tcgcgaccaa tctgccgctt tcgactgcca caaagatgac
     4321 cagaaatggc gtcgacgccc tcgagccgcg tatggtgcgc gagctgggtc tcgaccggcc
     4381 gccgcccgaa cctgtgttcg cgagcatggc agccgatata cgcgacgctc attgcggcta
     4441 tgcccctgag gaaatcgtga aaccaatggt ggatgtccag cgggccagag acgcccagat
     4501 ggccgagagt ttgatcgccg ccgccgacgc cgacggcgcg gtcctggtgg ccggagcggg
     4561 tcatgtccga aacgattacg gcattcccgt tcatctcaga gcaaaggcac cgaaaaaaca
     4621 ggtgatcagc atcgctttcg tcgaagtcga taaacaacag accgcaccgc aaagttacgc
     4681 gctgccgtac ccgaatgacc gattgccttt cgactacgtt tggttcacgc ctcgcgtcga
     4741 cgccgaagac ccttgtgaaa agttcaagtc ccacttcgat cgaat
//
LOCUS       DASULZ010000611         6859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_171848, whole genome shotgun sequence.
ACCESSION   DASULZ010000611 DASULZ010000000
VERSION     DASULZ010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6859
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171848"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..1100
                     /locus_tag="VFY96_12580"
     CDS             162..1100
                     /locus_tag="VFY96_12580"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5607349.1"
                     /translation="MNYRQLGHAGVRVSVVGLGANRFGSPDVSQAEVDKIIDAALDAG
                     INFIDTADVYNDGASEETLGQALKGRFHRVVMATKFNFPRMTSANSWGASRYHMMQSV
                     ELSLRRLQTDHIDLYYCHRWDDATPIEETLRGLDDLIRMGKVHYIGASAYASWQLAHA
                     NLLAELKSWSRFVVLQSEYHMLSRAVESEVLPYCRAHHVGFVPYYPLAGGFLTGKYEL
                     GKPAPRGSRGESARSVQELMTEKNYQVVTRLSGWAKDHARGINELAQAWLLAQPSVCS
                     VITGARKVEHVTSNVKAADWNLNREELKEIEAILND"
     gene            complement(1124..1699)
                     /locus_tag="VFY96_12585"
     CDS             complement(1124..1699)
                     /locus_tag="VFY96_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607350.1"
                     /translation="MPISFTPPMKQRVTRRTVAMLQRSLDYFPELRDQSITVGYTRKH
                     LGSATVIYRKGVLSQLIIRLRVRKVAYQTIGHELTHLIQALAYGDRSGKRSSIPGRIP
                     SGEKQCDIWTLAREPLFCDDAPTYIKMPRIVRDHWPDYAGAVRDLCISAITKRHTHRQ
                     YIRWLEDEIAKLPASQARKKPATPTQLMLPW"
     gene            1775..2695
                     /gene="era"
                     /locus_tag="VFY96_12590"
     CDS             1775..2695
                     /gene="era"
                     /locus_tag="VFY96_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX5607351.1"
                     /translation="MAETDHKNKENLAFRSGYVAIIGRPNVGKSTLLNRILGEKVAIV
                     SPKPQTTRNRITGIRTTESSQIIFLDTPGIHQGHSLINRRLMDTALQTLDEVDGVLWL
                     LDAHDKIKQEEERIAETLSGVQTPVLILLNKIDVVSKGKLLPLIERCAQLLPEKEIVP
                     VSALRGEGIDLVLRLIEGWLPEGPRYFTEGEYTDQSERFLASEIVREKVFLLTREEIP
                     YGVAVTIDEFTEKTEKNLLVISATIHTDRDSHKGILIGKRGSMLKEIGKQAREELEAL
                     LGCRIFLELFVRVDPGWTQNPHLLSEMGVG"
     gene            2692..4098
                     /gene="der"
                     /locus_tag="VFY96_12595"
     CDS             2692..4098
                     /gene="der"
                     /locus_tag="VFY96_12595"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX5607352.1"
                     /translation="MSRALSTTRRALHSRPRKLPVVAIVGRPNVGKSTLFNRLVGERR
                     AIVDDFPGVTRDRNYAEAVWGGRQYILIDTGGLDTESNGGLEASVQEQSRRAVDEADV
                     VVFLFDGKSGLNPLDAEAVDLLRVSQKPVLYAVNKLDSQRRADNVYEFHALGLDTLYS
                     ISAEHGLGVADLLDDMVARFPTLPAVTDETAEEQIEDKVRPLRVAVVGRPNVGKSTLV
                     NRLLGYERSVVHATPGTTRDALDTPFDLAGEACVLVDTAGIRRKAKIDDRIERFSVGR
                     SLKAVDRSDLVIHVIDGPEGVTDQDAQILSYAVDRAKALVLAVNKWDLVSNSGGHPEK
                     YRAEVRYRLSFLEFAPIALISATTGYGVRKLLETAARLVRNYRRKTSTSAFNQALQQI
                     TRAHSAPLAQGRAVKFYYGTQTAAGPPTFTVFVNRPDEVPESYKRYLLHQLREKLGLE
                     NTPLRLYLRGRREERSTS"
     gene            4311..5459
                     /gene="dprA"
                     /locus_tag="VFY96_12600"
     CDS             4311..5459
                     /gene="dprA"
                     /locus_tag="VFY96_12600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX5607353.1"
                     /translation="MIEPALPLGKGAPLSGHDCAPWLALGRIKGLGGVSLKKITARFA
                     DAAAVFRASVAELAEVEGLHRDLIDDIANFTDWAEIEKEIQRAHAAGIQMIPFSDAAY
                     PARLRAIADPPALLYVKGEIRGNDERAVAMVGSRSASDYGRRVAHDLARGLVSFGFTV
                     VSGMARGIDGMAHESAIRAGGRTLAVLGCGVDRAYPPEHEMLYRRISESGAVISELPM
                     GARPIAFNFPARNRLISGLALGVVVVEATEKSGSLITASLAAEQGREVFAVPGEAGAS
                     RSRGSHRLIRQGAKLVESVEDIIEEIAPQLTRTSGDSEARSARTLPENSGPAARQIFA
                     LLKESSLQIDQLIEQTGLPAPQVLQILLDLELQGFVCQSPGKIYRAEK"
     gene            5503..>6859
                     /gene="topA"
                     /locus_tag="VFY96_12605"
     CDS             5503..>6859
                     /gene="topA"
                     /locus_tag="VFY96_12605"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX5607354.1"
                     /translation="MAAKNLVIVESPAKAKTLEKYLGRDFQVKASVGHVVDLPKSKLG
                     VDIKKDFTPDFHVIQAKKKVVDDLKKAARGKEHIYLASDPDREGEAIAWHIADQVAKN
                     HRRVHRVLINEITKKAVQEAIANPQQLDRNKFDAQIARRVLDRLVGYQISPLLWKKVR
                     RGLSAGRVQSVAVRLVCEREREIRAFESVEYWSLAAQLEGRLPPSFRARLFQWQGQKL
                     DNKNFRLENEAHVQQIIQSLEGASWTIGDVEKKERRRYPTPPFVTSKLQQEAARKLGF
                     QPKRSMQLAQQLYEGVELGNEGSVGLITYMRTDSTRISADALTAVRQHIQARYGKDYV
                     PGKPNTFRSKKGAQDAHEAIRPTSLEYTPERVRRYLRRDLFQLYSLIWDRFVASQMVP
                     AVYDQTLFDIPVREALFRATGQQIKFDGFMKVYIEGRDERVSANGEDDGEDATDDSTG
                     VL"
BASE COUNT         1531 a         1799 c         2087 g         1442 t
ORIGIN      
        1 aaagaaaccg caacgcgtaa ttcgagcgat gccaagctcc gcgcgcaatc gatagcccat
       61 ctatcttttc catgaacacg cgcctctgta ggggccagtc ttccgcgtcg cagcggatgc
      121 gctggtaacg catccatccg taattgtcga aggagttttc catgaattac cgtcaactgg
      181 gccatgccgg cgtccgggtt tcagtggtcg ggcttggcgc caatcgtttc ggctcgccgg
      241 atgtgtcaca agcagaggtc gacaagatta tcgatgcagc gctcgatgcg gggataaact
      301 tcatcgatac ggccgacgtc tacaatgatg gcgcttccga agagactttg gggcaggcac
      361 tcaaagggcg ctttcatcgc gtggtgatgg cgaccaaatt caattttccg aggatgacca
      421 gcgccaacag ctggggtgcc tcgagatatc acatgatgca atccgtcgag ctgagcttgc
      481 gtcgcttgca gaccgatcac atcgatctct attactgcca tcgctgggat gatgcgacgc
      541 cgattgaaga gacattgcgc ggcttggacg atctcattcg tatgggcaaa gttcactaca
      601 tcggcgcatc cgcctatgca tcatggcaac tcgcgcacgc caacttgctt gctgagctaa
      661 aaagttggag ccgttttgtt gtgctgcaat cggagtacca tatgctgagc cgcgcagtgg
      721 agagcgaagt gttgccgtac tgccgcgctc atcacgtcgg gtttgttccg tactatccct
      781 tggcaggcgg ttttctcaca ggaaaatatg agctcgggaa acccgctccg cggggctcgc
      841 gtggtgagtc ggcgagaagt gtgcaagagc tgatgacgga aaaaaattat caagtcgtaa
      901 cgcggctgtc cgggtgggca aaggatcacg ctcgcggcat caatgagctg gcgcaggctt
      961 ggttgctcgc gcaaccttcg gtttgctcag tgatcaccgg cgcgcgaaaa gtagaacacg
     1021 taacgagcaa tgtgaaggcg gctgactgga atttaaaccg ggaagagctc aaagagatcg
     1081 aagcgatact caacgattag cgctaaccat gctgcgcagc gcgtcaccat gggagcatga
     1141 gctgtgtggg ggtggcaggt ttttttcgag cttggctagc gggcaattta gcaatttcat
     1201 cctccaacca gcggatgtac tggcggtggg tatggcgctt ggtgatggcg ctgatgcaga
     1261 ggtcacgcac cgctccggcg tagtccggcc aatggtcacg tacgatgcgc ggcatcttga
     1321 tgtaagtggg agcatcgtcg caaaacaatg gctcacgcgc gagcgtccaa atgtcacatt
     1381 gtttttcgcc ggaagggatt ctgccgggga tcgaagagcg tttgcctgat cgatcaccgt
     1441 aggccagcgc ctggatcagg tgcgttagct catggccgat ggtttgatag gcgaccttgc
     1501 gaacgcgcaa ccggatgatt aactgcgaaa gcaccccctt gcggtaaatc accgtagcgc
     1561 tgccaagatg tttccgcgta taacccaccg taatgctctg gtcgcgtaat tcgggaaaat
     1621 agtctaagga gcgctgaagc atcgccacgg tccggcgagt gacacgctgt ttcatcggcg
     1681 gcgtgaagct aatcggcatg aaacatggtg caggagcgca aacttgaacg gacagggctg
     1741 ggtgtcataa tactggcgcg ctgcaggccc tcttatggca gagaccgatc ataagaacaa
     1801 ggaaaatttg gcgtttcgct ccggctacgt cgcgatcatc ggtcggccga atgtcggcaa
     1861 gtcaaccttg ctcaatcgca ttctcggcga gaaggttgcg atcgtctcgc ccaagccgca
     1921 aacgacccga aaccgcatca ccggcatccg cacgacggag tcctcacaga ttatattttt
     1981 ggatacgccg ggaattcatc agggccactc attgatcaat cgaaggctga tggacacagc
     2041 gttgcaaacc ttggacgaag tcgacggcgt gctgtggctg ctcgatgcgc atgacaaaat
     2101 taaacaggaa gaagagcgca ttgcggaaac cttatccgga gtgcaaacac cagtgctgat
     2161 tttgctgaac aagatcgatg tggtttccaa aggcaaatta ctgccgctga tcgaacgctg
     2221 cgctcaactg ttgccggaga aggagatcgt gccggtaagt gcgctgcggg gcgaagggat
     2281 cgatctcgtc ttgagactca tcgaaggatg gttgcctgag ggaccgcgct actttacgga
     2341 gggcgaatac accgatcaat cggaacgctt tctcgcttcg gagattgtcc gggaaaaagt
     2401 attcttgttg acccgcgagg aaatccccta tggcgtggca gtgaccatcg atgagttcac
     2461 cgaaaaaacg gaaaagaatt tgctcgtgat tagcgcgacg attcataccg accgcgattc
     2521 gcacaagggg atactgatcg ggaagcgcgg cagcatgcta aaagagatcg gcaagcaggc
     2581 gcgcgaagaa ctggaggcgc tcctcggttg ccggattttt cttgaactct tcgtccgagt
     2641 ggatccgggt tggacgcaaa atccgcactt gttatccgag atgggtgtgg gatgagtcgc
     2701 gcgctttcca ccactcgccg tgcgcttcat tcgcgtccgc gcaaacttcc cgtcgtggcg
     2761 attgtcggtc gaccgaacgt cggcaagtcg accttgttta accgtctcgt gggcgagcgc
     2821 cgagcgatcg ttgacgattt tcccggagtg acccgcgacc ggaactacgc cgaggccgtt
     2881 tggggcgggc gacaatacat cctaatcgac accggtgggt tggacaccga atcaaacggc
     2941 gggctcgagg cgagcgttca agagcagagc cgccgggcgg tggacgaagc ggacgttgtc
     3001 gtctttctct tcgacggcaa gagcggcctc aatcctctag atgcagaagc ggtggatctg
     3061 ctccgggtat cgcaaaagcc cgtcttgtat gcggtcaata agctggactc gcaacggcgg
     3121 gcagacaacg tctatgaatt tcatgccctc ggcctggata ctctctattc gatttccgcg
     3181 gagcacgggc tgggcgttgc ggatctgctg gacgacatgg tggcgcgttt tccgaccctt
     3241 ccggcggtca ctgacgaaac agcggaagaa caaatcgagg ataaggttcg ccccttgcgt
     3301 gtggcggttg ttggtcggcc gaacgtgggc aaatcgactt tggtcaaccg tttgttgggt
     3361 tatgagcgct cggtagtcca tgccacccca ggaaccacgc gggacgcgtt ggacacccct
     3421 ttcgatcttg ccggtgaagc atgtgtcctg gtcgatacgg cgggcattcg gcgcaaggca
     3481 aagatcgacg atcgtatcga acgtttcagc gttggacggt ctttgaaggc ggttgaccgc
     3541 agtgatctcg tgatccatgt gattgatggg cctgaaggcg tcaccgacca ggacgctcag
     3601 attcttagct acgccgtgga tcgcgcgaaa gccctcgtcc tcgctgtgaa caagtgggat
     3661 ctggtctcga atagcggcgg ccacccggag aaataccgcg ccgaagtccg ttatcgcctt
     3721 tcgtttttgg agtttgcgcc gatcgcgctg atttcggcaa ctaccggtta tggagtgcgc
     3781 aagctcctcg agacggcggc gcgcttggtg agaaattatc gccgcaagac ttcaacgtca
     3841 gcattcaatc aggcattgca gcaaatcacc cgggctcatt cggcgcctct cgcgcagggg
     3901 cgcgcagtga aattttatta cggcacgcaa acggcagctg gaccgccgac cttcaccgtt
     3961 tttgtcaatc ggccggacga agtgccggaa agctacaagc gctatctact ccatcagttg
     4021 cgggagaagc tcggcctcga gaacacaccg ctgcgcttgt acttgcgcgg ccggcgtgaa
     4081 gaacgatcca cgtcatagct gcgcaaacgt cgtcaagtaa caaggggatc aatcgcagtt
     4141 tagagcgcgt ggaggccaac gcgggtattt cggctgcgcc gatagttcgg agatcgatga
     4201 ggagagctta tagcgttcaa gggcaaatta ccttatcatc tatatttcgt ttctctggcc
     4261 gcgcttgcga ctgagttcac gcgctccagt ttgacaaagc ttcgacaatt atgattgagc
     4321 ctgctctacc tttaggaaaa ggcgctcccc tgtcgggcca cgactgcgcg ccatggctcg
     4381 cgttgggaag aattaaaggg ctcggcggcg taagcttaaa aaaaatcacc gcacggttcg
     4441 ccgatgcggc ggcggttttt cgcgcgtccg tggctgagtt ggcggaggtc gagggactgc
     4501 accgcgatct tatcgatgat atcgcgaact ttactgactg ggcggagatc gaaaaagaga
     4561 ttcaacgcgc gcacgcggcg ggtatccaga tgattccatt cagcgatgct gcctaccctg
     4621 cgcgattacg ggccatcgct gatccgccag cgttgcttta tgtcaaagga gaaattcgcg
     4681 gcaacgacga gagagccgtt gccatggtcg ggtcgagaag cgccagtgat tacgggcgtc
     4741 gagtcgcgca cgatctggca cgcggcttgg tctctttcgg ctttacggtg gtcagcggca
     4801 tggcgcgcgg catagacggc atggcgcatg aaagcgcaat ccgggcgggc ggccgtacgc
     4861 tggcggtgct tggttgtggg gtcgatcgcg cctacccgcc ggagcatgag atgctttatc
     4921 ggcggatcag cgagagcggc gcggtgatct cagagttgcc catgggcgcg cgtccgatcg
     4981 catttaattt tccggcgcga aatcgtctga tcagtggact cgcgctgggc gttgtcgtgg
     5041 tcgaagccac cgaaaagagt ggctctctga tcaccgccag tttggccgcg gagcaaggta
     5101 gagaagtttt tgccgtccct ggggaagcgg gcgcgagccg cagccggggc tcgcatcgtt
     5161 tgattcggca aggcgccaag ctggtcgaaa gcgtcgaaga tatcattgaa gagattgccc
     5221 cgcagttgac gcgaacgagc ggcgacagtg aagcgcgttc ggcacgcact ctgccggaaa
     5281 attcgggccc ggcggcacgg cagattttcg cactgctcaa ggaaagttcg ctgcaaatcg
     5341 atcaactgat cgagcaaacc ggcttgccag cgcctcaagt gctgcaaata cttttggatt
     5401 tggaattgca gggattcgtc tgtcaaagcc cgggaaaaat ctatcgagcg gaaaaatagc
     5461 tctacaacca ggattgaaga attgaggaat taagcttaag tcatggcagc caaaaattta
     5521 gtcatcgttg agtcaccggc gaaggccaag actctggaaa aatatttagg tcgcgatttt
     5581 caagtcaagg cgtcggttgg tcacgtggtc gatttgccaa agagcaaact cggcgtcgat
     5641 atcaagaaag attttacgcc ggacttccat gtgatccagg cgaagaaaaa agtcgtcgac
     5701 gatctcaaaa aagccgcccg cggaaaagag cacatctatc tcgcctccga tcccgaccgc
     5761 gaaggggagg cgatcgcctg gcatatcgcc gatcaggtgg caaaaaatca tcgccgcgtg
     5821 catcgggttc tgatcaacga gatcaccaaa aaagcggtgc aggaggcgat cgcgaatccg
     5881 cagcaactgg accgaaacaa gttcgatgca cagatcgcgc gccgcgtttt ggaccgtttg
     5941 gtcggctatc aaattagtcc gctgctttgg aaaaaagtcc ggcgcgggtt gagcgccggg
     6001 cgcgtgcagt ccgtggcggt gcggctggtt tgcgagcggg agcgggaaat tcgcgctttc
     6061 gaatcggttg aatactggtc gctggcagcg cagctcgaag gccggttgcc gccatcgttc
     6121 agggcgcggt tgtttcagtg gcaaggacaa aagctcgaca ataagaattt tcgcctggaa
     6181 aatgaagcgc atgtccaaca gatcattcaa tcgctggaag gcgcatcttg gaccatcggc
     6241 gacgtcgaga aaaaggaacg gcgtcgttat ccgacgccgc ccttcgtcac tagcaagctg
     6301 caacaagaag cggcgcgtaa gcttggtttt caaccgaagc gctcgatgca gctggcgcag
     6361 cagctctacg aaggagtgga gttgggcaac gagggctcgg tcggcttgat cacttacatg
     6421 cgtaccgatt cgacgcgcat ttccgccgat gcgctcacgg ctgtgcggca acacattcag
     6481 gcgcgctacg gaaaggatta cgttcccgga aaaccgaata cgtttcgctc gaaaaagggc
     6541 gcacaggacg cgcacgaggc gattcggccg acatcgctgg aatatactcc ggagcgggtg
     6601 cggcgttact tgcgacgcga tttgttccag ctctactctt tgatttggga ccggtttgtc
     6661 gccagccaaa tggtgccggc agtatacgat cagacgctct tcgacattcc cgtccgggaa
     6721 gcgctgtttc gcgccaccgg acagcagatc aagttcgatg gtttcatgaa agtctacatc
     6781 gaaggccggg acgagcgtgt ctccgccaac ggtgaggacg atggcgaaga tgcgacggat
     6841 gattcaaccg gcgtgctgc
//
LOCUS       DASULZ010000612         7483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_679349, whole genome shotgun sequence.
ACCESSION   DASULZ010000612 DASULZ010000000
VERSION     DASULZ010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7483
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679349"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="VFY96_12610"
     CDS             complement(<1..938)
                     /locus_tag="VFY96_12610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX5607355.1"
                     /translation="MMQRAVIVSACRTPIGSFQGKLSRLSATQLGSVVIKEALDRTHL
                     TGDQVDEVIMGNVLSAGLGQAPARQAGLGAGLPRAVTCTMINKVCGSGLKAVMCAAQA
                     ITSGDAEVVIAGGMESMSNAPYLLTKARSGYRLGSGELVDSLIRDGLWDVYNQFHMGA
                     AGELCARTLKISREEQDAVAATSYQRAQRAQAEGAFRRELIPIAVPAGSNEDLVAEDE
                     EPKRLNFSKLAQLSPSFEKNGTITAGNASSISDGAAAVVVMSEEKARLLHIEPLAKIV
                     GQTSYGTDPAWFTTAPAKAITRLMERTGRSVQSVDL"
     gene            1341..2420
                     /gene="pdhA"
                     /locus_tag="VFY96_12615"
     CDS             1341..2420
                     /gene="pdhA"
                     /locus_tag="VFY96_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX5607356.1"
                     /translation="MPKTLTKDFRIEWLQILDENGNCDQSLRPPLTDEQIKVLYENLV
                     FARAFDEKAFKLQREGRLGTYASILGQEAAQVGSAFALDPGDWMFPSFREPGASFVRG
                     LPPHMIFQYWAGDERGSKIPEGQNDFPITIPVGTQIPIGAGAAWGAKLRGDKIAVLVY
                     LGDGATSKGDFHEGFNLAGVFSLPVVFLCQNNQWAISVPLKRQTAAQTLAQKAIAYGF
                     PGIQVDGNDVFAVYKATHEALARARAGGGPTFIEAVTFRIGDHTTADDASRYRNKETV
                     EEWKRKDPIDRLIRYMISQGLWDEAYGQQTVAKGRERVEAAVKEFETVTPPKPKDIFR
                     FTFAEMTDELREQMEALLRGNGRVH"
     gene            2441..3418
                     /locus_tag="VFY96_12620"
     CDS             2441..3418
                     /locus_tag="VFY96_12620"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX5607357.1"
                     /translation="MAKLTVVEALNQALRQAMVRDDRVIVLGEDVGKNGGVFRVTQGL
                     LDQFGENRVIDTPLSESAIIGASIGLAVYGLRPIPELQFEGFAFGAMEQLVAHASRIR
                     NRSRGRYQCPMVVRIPYGGGIHAPEHHSESNEAYFVHTPGLKVVVPSTPYEAKGLLLA
                     AIQNPDPVIFMEPKKIYRSIREEVPEEEYTIPLGQARVVIEGNSVSLIAWGAMLHVAL
                     EGARLMADRGINCEVLDLRTLSPLDTDAIIESVRKTHRAVIVHEAQRSCGLAAEIAAR
                     INEKALLDLEAPVERVTGFDTVMPLPKLERQYLPDARRVADAIDRVMSA"
     gene            3438..4625
                     /locus_tag="VFY96_12625"
     CDS             3438..4625
                     /locus_tag="VFY96_12625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX5607358.1"
                     /translation="MIKEFRLPDVGEGISEAEIVRWLVADGATVNEDQDIVEIETDKA
                     IVALPSPHSGKIAKLHRKQGDLAKVGEILVSIETEDTGPGAPQADLGTVVGSLGDQEV
                     IELPTPVKATPAVRALAKQLGIDLVKIRGTGSEGKITREDVERAAAGTAAEERHDGES
                     SGPIERIPLRGLRRTIAKRMHAARQRVSDVTIWEDADITALDRIRSEEKAIAAQKGIK
                     LTYLPYVIQATIDALKVHPYLNASLDDEREEILLKKFYNIGIAVDTADGLIVFAIKQA
                     DQKSLLELAQEISTLAEKARTRRLDLSELRGSTFTITNYGVIGATYGTPIINYPEVGI
                     LGIGRIQDRPVVDDGAIAIHKIMPLSLVFDHRVVDGVEAARFLNVIIDRLENPKETLA
                     EKS"
     gene            complement(4733..5659)
                     /locus_tag="VFY96_12630"
     CDS             complement(4733..5659)
                     /locus_tag="VFY96_12630"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5607359.1"
                     /translation="MTSDICKVGVVGIGLMGSGIAQVAAAKNFDTVVVDVSAEIIAKG
                     MERIQASVRRMVESYQKTSGKSGIAPDEKDGIIARIKPSTERASLSDCDIIIEAMIEN
                     EAAKKQLILSLAQSGYQKLLVSNTSSISITRLASAYSAPEKFMGMHFMNPVPVQPGCE
                     LIRGLLTSNQTWESVSAFCRELGKEPISAEDKAGFGINRMFVPFLMEAVKIVEEGVMS
                     CEDADKATLCLGHKMGPISTLDFVGLDTTLAIADVLRDELGPSYRAPDLLCKLVAAGF
                     YGVKTGKGFYLWEQGKKIRVNPAVARYRRAKI"
     gene            5882..7069
                     /locus_tag="VFY96_12635"
     CDS             5882..7069
                     /locus_tag="VFY96_12635"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX5607360.1"
                     /translation="MATATENTAVILAAARTPFGKFGGALRTLTATDLGVLAAQAVVK
                     RSGVRAESIDHVIFGNALQTSSDAIYLARHIALRAGIPQSVPGLTLNRLCGSGFQSII
                     TGAQMLLLDEADFVLAGGTESMSQAPYVLRNSRWGMPLGKEEKLEDSLWSALTDSYCE
                     LSMAQTAEKLGNRYGVTRQQADEFAYQSQNKAKLANQCGRLAEELAPVEGVLQYDEHP
                     RPETTREALAALPPVFKENGLVTAGNASGICDGAAAVILTTESKAKAHDLAPLAKIIS
                     WGIVGCDPSIMGIGPASAIRTSLNRARLRLDQIDLIEVNEAFAAQYLAVERELGLNRE
                     RVNVNGGAIALGHPLGATGARITTTLTYELRRRGARFGLGSACIGGGQGIALLIENIG
                     QGV"
     gene            7092..>7483
                     /locus_tag="VFY96_12640"
     CDS             7092..>7483
                     /locus_tag="VFY96_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEX5607361.1"
                     /translation="MNHTETAAGSIDQERLKNLLIELIQIDSLSRKELNVALRLKREL
                     EEVGATVLIDDAGEKVGGNVGNLIAHFPGNRSAAEPLLLSAHMDTVVPGEGIVPILEG
                     DILRTDGRTVLGGDDKSGVAIICEVMRV"
BASE COUNT         1671 a         2146 c         2079 g         1587 t
ORIGIN      
        1 aacaagtcca ctgactgaac gcttcggccg gtgcgttcca tgagacgggt gatcgccttg
       61 gccggagcgg tggtgaacca ggcagggtcc gtgccgtagc ttgtctggcc gacgattttc
      121 gccagcggct caatgtgtaa gaggcgggct ttctcctccg acatcaccac gacagccgcc
      181 gcgccgtcgc tgatggaaga agcgttgcca gcagtgattg tgccgttttt ttcgaatgac
      241 ggactcaatt gagcgagctt gctaaaattc agccgttttg gttcttcgtc ttctgcaacg
      301 agatcttcgt tagacccagc gggcacggct atcggtatca actctcgcct gaaagccccc
      361 tcagcctgcg ctctctgtgc ccgttggtaa ctggtggcgg caactgcgtc ttgttcttcc
      421 cggctgatct taagcgttcg ggcgcagagt tcgcccgctg ctcccatgtg aaattgatta
      481 tagacgtccc acagtccgtc acggatcaaa ctatcgacca gttctccgga acccagccga
      541 taaccgctac gagccttcgt cagcagatag ggagcgttac tcatactctc catgccgccc
      601 gcgatgacta cttcggcgtc acccgacgta atcgcctgag ccgcgcacat cactgccttc
      661 aatccagatc cgcatacctt attgatcatt gtgcaagtca ctgcgcgggg gagccccgcg
      721 ccaagaccgg cttggcgtgc gggcgcttgg cccaaaccgg ccgacagcac gttgcccatg
      781 atgacttcat cgacttgatc gcccgtcaga tgcgttctgt ccagcgcttc tttgatcacc
      841 acactgccta actgtgtggc cgacaggcgg cttaattttc cctggaagct gccgatcgga
      901 gtgcggcagg cgctaacaat gacggcgcgt tgcatcatga cctccgcttt tcgcgcaagg
      961 catcagggga aatgctgaaa aactttctaa ctgagatgat gaagaggcta tcgtgtcgga
     1021 aatttgaaaa ttgccaggct cttgcgcggg agctttgcga tagatgaaag cttgagcaac
     1081 ggacttgccg cagcgtccgc aactgccagt cttgcggagc tggtgcattg aagagagatt
     1141 cggagcttct cgctgaccca tcacgccgag ctcctttccg gctgactgcg gccgcgttcg
     1201 tctacttcat agtttaccag aaaaacgaag tctcggaagc acaaattcgt gccctccgca
     1261 aataccacat tcactttccg gttcggagtg gtatacttcc ttgcgtatgc gacgggaata
     1321 gagccaagaa aggatccgcc gtgcctaaga cgctcaccaa ggatttcagg atcgaatggc
     1381 tccagattct cgatgagaac ggcaattgtg atcagtccct ccgccctccc ctcaccgatg
     1441 agcaaatcaa agttctgtat gaaaatttag ttttcgcccg tgccttcgac gagaaagctt
     1501 tcaagctcca gcgcgaagga cggctcggca cctacgcttc gatactcggc caagaagccg
     1561 cgcaagttgg gagcgcgttc gcgctggacc cgggcgactg gatgtttccc tccttccgcg
     1621 agcccggcgc atcgttcgtc cggggtctac cgccgcacat gatttttcaa tactgggccg
     1681 gtgatgagcg aggcagcaag attcccgaag gacaaaacga ctttccgatc acgattccgg
     1741 tgggaacgca gattccgatc ggtgccgggg ctgcctgggg cgcaaaactc cgaggagaca
     1801 agattgcggt gctggtttat ctcggcgatg gcgccacctc caagggagat tttcacgaag
     1861 gttttaatct cgccggcgtt ttttctctgc ccgtagtctt tttgtgccag aacaatcagt
     1921 gggcgatatc ggttccgctc aaacgtcaaa ccgccgcgca aacactggcg caaaaggcga
     1981 tcgcttacgg tttccctggc attcaagtcg acggcaacga tgtttttgcg gtgtacaaag
     2041 ctactcatga agcgctggcg cgcgcccgcg ccggtggcgg gccgacattt atcgaggctg
     2101 tcaccttccg gattggcgat cacaccacgg ccgacgacgc atcacgctat cgcaacaaag
     2161 agacggttga agagtggaag aggaaagacc ccatcgaccg gttgatccgc tatatgattt
     2221 ctcaggggct gtgggacgaa gcctacgggc aacaaacggt agcgaagggc cgcgaacgag
     2281 tggaggccgc cgtgaaggaa tttgaaaccg tcacgcctcc caagcccaaa gatatctttc
     2341 gcttcacgtt tgcggagatg accgacgagt tgcgtgagca gatggaagct ttattgcgcg
     2401 gcaacgggcg ggttcattga cggattccgc ggaggcaaag atggccaagc tcaccgtcgt
     2461 cgaagcgctc aatcaggcgc tcagacaagc gatggtacgc gatgaccggg tgatcgttct
     2521 cggcgaagat gtcggaaaaa acggcggcgt cttcagagtc actcagggct tattggatca
     2581 gtttggcgaa aaccgcgtga tcgacacacc gctctcggaa tctgcgatca tcggagcctc
     2641 catcgggctc gccgtctacg gcctacggcc catcccagaa ctccagttcg aaggattcgc
     2701 cttcggcgcc atggagcagt tggtcgcgca tgcctcacgc atccgcaatc gctcgcgcgg
     2761 ccgttatcag tgcccaatgg tcgtgcgcat tccgtacggc ggcggcatcc acgcacccga
     2821 gcatcattcc gaaagcaacg aagcttactt tgttcacact ccaggtttga aagtcgtcgt
     2881 gccctctacg ccgtacgaag ccaagggttt gctgcttgcg gcgatccaaa atcccgatcc
     2941 agtcattttc atggaaccca agaaaatcta tcgttccatt cgcgaggaag tcccggagga
     3001 agagtacacg ataccgctcg gccaagcacg cgtcgtcata gagggaaaca gcgtgagttt
     3061 gatcgcgtgg ggcgccatgc tgcacgtcgc tcttgaaggc gcgcgcttga tggccgatcg
     3121 cggcataaac tgcgaagtcc tggatctccg cactctttct ccgctcgaca ccgacgcgat
     3181 cattgagtca gtgagaaaaa cccaccgggc ggtcatcgtc catgaagctc agcggagctg
     3241 cgggctggcg gcggagatcg ccgctcggat caacgaaaaa gcgctgctgg atttggaagc
     3301 gcctgtagaa cgcgtgactg gtttcgacac ggtgatgccg ctgcctaaat tggagcgtca
     3361 atatttgccg gatgctcgtc gcgtagccga tgcgatcgac agggtcatga gcgcgtaacc
     3421 ctgtcgcagc gaaagccatg atcaaggagt ttcgactgcc cgacgtcggc gaaggtatca
     3481 gcgaggcgga aatcgtacgc tggttggttg ccgacggcgc caccgtcaac gaagaccaag
     3541 acatcgtcga gatcgaaacc gacaaagcga tcgtcgcact gccttcccct cacagcggaa
     3601 aaatcgccaa attacatcgc aagcaaggcg atctcgccaa ggtcggcgag atcctggtgt
     3661 cgatcgaaac agaagacacc ggacccggcg caccgcaagc cgatctcggc acggtggtcg
     3721 gctctctcgg cgaccaagaa gtgatcgagc tgcccacacc ggtaaaagca actccggccg
     3781 tgcgcgctct cgctaaacag ctcggcatcg atcttgtcaa gattcgtggg acgggcagcg
     3841 agggaaagat cacccgcgaa gacgtcgaac gggctgccgc aggaacagcc gctgaagagc
     3901 gtcacgacgg cgagtcgtcc ggcccgatcg aaagaattcc gctgcgcggg cttcgccgca
     3961 ccatcgccaa gcgcatgcac gctgcccgtc agcgcgtttc tgacgtcacg atttgggagg
     4021 acgcggacat caccgccctg gatcgcattc gctcagagga gaaagcgatc gcagcgcaaa
     4081 agggaatcaa acttacttat ctgccttacg taattcaggc cacgatcgac gcgctgaagg
     4141 ttcatcctta cctcaatgcg agcctcgatg atgagcgcga agagattttg ctcaaaaaat
     4201 tctacaatat cggcattgcc gtggacacgg ccgatgggct gatagtcttc gccatcaagc
     4261 aggccgatca aaaaagcctg ctcgaactgg cgcaagagat cagcacactg gccgaaaagg
     4321 cgcgcacgcg aagactggac ctttccgaac ttcgcggcag cacgtttacg atcaccaatt
     4381 acggggtgat cggcgccacc tatggcacac cgatcatcaa ctaccccgaa gtgggcatct
     4441 tgggaatcgg ccggattcag gaccgccccg tcgtagacga cggcgctatc gcaattcaca
     4501 agattatgcc actctcactg gtgttcgatc accgagtggt cgatggcgtg gaggccgcgc
     4561 gctttttaaa tgtgatcatc gaccgcttgg aaaatcccaa agagacactg gcagagaaaa
     4621 gctagtctga agagaaattc tcctgaaagg cgccaacgcg caacgtaaga agtcttttcg
     4681 aatgttccga aatttggcgc cattgtggga aaatatttct tggcgtggag cgctagatct
     4741 tggctctacg gtagcgtgcc acggcgggat tgaccctgat ctttttacct tgctcccata
     4801 gataaaaccc cttgccagtt ttgacgccgt aaaaaccggc cgcgaccaac ttgcacagca
     4861 aatcgggagc ccgatagctc ggtccgagtt catcgcgcag cacgtccgca atcgccaagg
     4921 tcgtatccaa accgacgaag tccaaagtgc tgatcggccc catcttgtgg cctaggcata
     4981 aggtcgcctt atccgcatct tcgcagctca tgacgccttc ctcgacgatt ttaactgcct
     5041 ccatcaaaaa aggcacaaac atgcggttaa ttccgaatcc tgccttatct tcggccgaga
     5101 tcggttcctt gccgagttca cgacaaaaag ccgagacgct ctcccatgtt tggtttgaag
     5161 tcaggaggcc gcgaatgagc tcacagcctg gctgcaccgg caccgggttc ataaagtgca
     5221 tacccatgaa tttctcgggc gccgaatacg cgctcgccag gcgagtgatt gaaatcgacg
     5281 aggtgttgga aaccaggagt ttttggtagc cggattgagc taacgatagt atgagctgct
     5341 tcttagccgc ttcattttcg atcatcgctt caatgatgat atcgcaatct gacagactag
     5401 cgcgctccgt tgacggctta atgcgcgcga tgattccgtc tttttcatcc ggcgcgatgc
     5461 cggacttgcc gctcgtcttc tggtagcttt ctaccatccg gcgcacgcta gcctggatcc
     5521 gttccatgcc ctttgcgatg atttccgcgc tgacatcaac gaccacggtg tcgaagttct
     5581 ttgcggcggc gacctgagcg atgccgctgc ccatgagccc gatgcccacg accccaactt
     5641 tgcatatgtc cgacgtcata acgttgcctc ctttgttgac cgttaggctt ctgttttcgt
     5701 caaatacgct gaagggcaac caccgatcgg gcccttagtt tgcgtctttg cgttttgtcg
     5761 gaaatttcgg atttccttgg ttgcagcgta gtcatggaag gcccatctca atgcataaga
     5821 cccttcgcac gccggataat tctaaattgc cacgcgacgg ttttccatgt tacgttttcc
     5881 catggccaca gcgacagaaa ataccgccgt cattctggcc gctgcccgca cgccatttgg
     5941 gaagtttggc ggtgcattaa gaacgcttac cgccaccgat ctcggcgtgc ttgcggcgca
     6001 agccgtagtc aaaaggtcgg gcgttcgcgc ggagagtatc gatcatgtca ttttcggcaa
     6061 cgcgttacag accagttccg acgcgattta tttggcaaga cacatcgcgc ttcgtgccgg
     6121 cattccgcaa tcggttcccg ggctcacgct gaaccggcta tgcggatccg gctttcaatc
     6181 gatcatcacc ggtgcacaaa tgctgcttct cgacgaagct gattttgttc ttgcaggcgg
     6241 caccgaaagc atgagtcagg ctccctatgt gctgcgcaac tcccgctggg gaatgccact
     6301 cgggaaagag gaaaagctcg aggactcgct ctggtcggcg ctgaccgaca gctattgtga
     6361 gctctcgatg gcacagacag cagaaaagct cggcaaccga tatggcgtca cacgacaaca
     6421 agccgacgag tttgcctatc agagtcagaa caaagccaag ttggcgaacc agtgcggccg
     6481 tttagccgaa gagcttgccc cggtcgaggg tgtcctgcaa tacgatgaac atccgcggcc
     6541 agagaccaca cgtgaagccc ttgccgctct gccgccggta tttaaagaaa atggtctggt
     6601 cacggctggc aacgcgagcg ggatttgcga tggagcagca gcggttattc ttactacaga
     6661 atccaaagcc aaggcacacg acctcgcacc attggcaaaa atcatctcgt ggggaatcgt
     6721 tgggtgtgat ccaagcatca tgggaattgg accggcatca gcgattcgga caagcttgaa
     6781 tcgggcacgc ctccgtttgg atcagatcga tctaatcgag gtgaacgaag ccttcgcagc
     6841 tcaatatctc gcggttgaac gcgaacttgg tttgaatcgc gagcgggtaa acgtcaatgg
     6901 cggcgccatt gcccttggcc atccgctggg cgccaccggc gcacgcatca ccactaccct
     6961 tacgtacgaa ctccgccggc gcggcgctcg cttcggtctg ggcagcgcct gcatcggtgg
     7021 cggccaaggt atcgcgttat taattgaaaa catcggtcaa ggcgtataaa aaggcaagaa
     7081 aacttcgatt tatgaaccat actgaaaccg cggcgggttc gatcgatcaa gaacgtctca
     7141 agaatcttct gattgagctg attcaaatcg acagtctctc gcgtaaagag cttaacgtcg
     7201 ccttgcgcct gaaacgcgag ctggaagaag tcggcgcaac cgttctcatc gacgacgcgg
     7261 gggaaaaagt gggcggcaac gtaggcaact tgatcgctca ttttcccggc aatcgttcgg
     7321 cggccgaacc gctgctgctt tccgcccaca tggacaccgt agttccgggt gaaggtatag
     7381 tgccgatctt ggaaggagac attctgcgca ccgacggccg cactgtgctc ggcggcgatg
     7441 acaagagtgg cgttgccatt atctgcgagg tcatgcgggt gat
//
LOCUS       DASULZ010000613         3282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_983868, whole genome shotgun sequence.
ACCESSION   DASULZ010000613 DASULZ010000000
VERSION     DASULZ010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983868"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1325)
                     /gene="murA"
                     /locus_tag="VFY96_12645"
     CDS             complement(57..1325)
                     /gene="murA"
                     /locus_tag="VFY96_12645"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX5607362.1"
                     /translation="MDKIIVQGGKRLTGEVTVSGSKNAALPILIASLLTAEPCRYQRV
                     PSLADIHTTLKLLGGLGVKIDKEGWARRTGELTLQAQTVTKLEASYDLVKTMRASFLV
                     LGPLTARFGQARVSTPGGCAIGARPVNLHLKGLESMGATIEHSHGYIEATAVRLRGAK
                     IYLDLPSVGATENLMMAATLAEGTTVIENAAKEPEIEDLAHALNKMGASVRGAGTDIV
                     QIDGVDSLHGFSHEIIPDRIEAGSFVIASAITGGDVWIKGARAEHLDAFLIKLQEAGI
                     ALRADRGGIRVQRNGRIKSVDVTTLPYPGFATDLQAQMMVLMSVADGVSIITETIFEN
                     RFMHVQELDRMGAQIQLEGNRAVVRGTRELSGAPVMATDLRASISLILAGLVASGSTE
                     VSRVYHLDRGYEHIEEKLSNLGAQIERVKA"
     gene            complement(1334..2257)
                     /gene="prmC"
                     /locus_tag="VFY96_12650"
     CDS             complement(1334..2257)
                     /gene="prmC"
                     /locus_tag="VFY96_12650"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX5607363.1"
                     /translation="MTSMETDSQSLGSLLGPRTIRCDLVDGTRRLQAAGIESARLDAE
                     VLMCHALRIKKSRLYVGLDEQPESSARQRFSNFLIRRLRREPVAYITGRQEFWSLDFL
                     VTPDVLIPRPETERLVEISLQCAGIFDNDLPLRILDIGTGSGAIAVSLAKELPRAQVL
                     AIDSSRAALEIARDNAARHQVADRVHFRASNLFESIRGREFHLIVSNPPYVESGEFAA
                     LAPEVSEWEPRTALDGGVDGLHYYRDIVAQAFRHLPPGGALIMEIGAAMARAVADLLS
                     GAGNYTAPLVHKDYGHRDRVIVAHAASSKLA"
     gene            complement(2257..>3282)
                     /gene="prfA"
                     /locus_tag="VFY96_12655"
     CDS             complement(2257..>3282)
                     /gene="prfA"
                     /locus_tag="VFY96_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005995114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX5607364.1"
                     /translation="ELERLMSDPGLANLQREYSKLAKERSELEEIVVSYREWRKVEQE
                     IQENRQLLEENDEAIRELAKEEVSALRQREEELENRLKYLILPKDPNDSKNVIIEIRA
                     GTGGDEAALFAGELYRMYSRYAESRGWRVETLTSSPTGLGGFKEIIVMVEGKGAYSRL
                     KFEGGVHRVQRVPVTEGSGRIHTSAVTVAVLAEADEVELDIDPKDIRIDVFRSSGPGG
                     QSVNTTDSAVRITHIPSGIVVSCQDEKSQHKNRAKGMKIMMARLLEKKQEEQRSEIAA
                     TRKLMVGSGDRSERIRTYNFPQGRLTDHRINLTLYQLERIMEGQIDEIVDGLITHYQA
                     EALKATG"
BASE COUNT          702 a          976 c          850 g          754 t
ORIGIN      
        1 taaccttcac gaaatcgatt ctctttccgg gagaaataga aactccagcg ggttacttac
       61 gctttaaccc gctcgatctg cgcgcccaaa ttcgataatt tttcttcgat atgttcatag
      121 ccgcggtcca agtggtacac cctggagact tcagtggacc cgcttgccac cagtccagca
      181 agaatcagcg aaatactagc gcgcaaatcc gtcgccatca ccggcgctcc cgaaagctcc
      241 ctggtgccgc gaacaacggc gcgattgcct tcgagctgga tctgggctcc catacggtcc
      301 aactcctgca cgtgcataaa ccggttttca aagatcgttt ccgtgatgat gctaacgccg
      361 tcggcgaccg acataagaac catcatctgg gcttgcagat cggttgcgaa cccaggatag
      421 ggtagagtcg ttacatcgac gcttttgatt ctgccatttc gctgcaccct gattccgccg
      481 cggtcagcac gaagcgcgat gcccgcctcc tgcaacttga tcaggaaagc atccaagtgt
      541 tcggcgcgcg cgcctttgat ccagacatcc ccgccggtga tggcgctggc gatcacgaag
      601 ctgcccgctt cgatgcgatc gggaataatc tcgtgcgaga atccatgcag ggaatctacc
      661 ccgtcgatct gaacaatgtc ggtgcccgca ccacgaacgc tcgcacccat tttgttgagc
      721 gcatgggcca gatcctcaat ctcgggttcc ttagcggcat tctcgataac cgttgtaccc
      781 tccgcaagcg tcgccgccat catgagattt tcggtggctc caacggaagg caagtcgaga
      841 tagatttttg cgccgcgcag ccgaaccgcg gttgcctcga tataaccatg ggagtgctcg
      901 atggtcgcgc ccattgattc aaggcctttg agatgcaaat tgaccggacg tgcgccgatg
      961 gcgcagccgc caggagtcga gactctggcc tggccgaagc gagccgtcaa aggtcctagc
     1021 accaagaacg aagctcgcat ggttttgacc aagtcgtaag aggcttcaag tttagttacc
     1081 gtttgggcct gcaatgtgag ctcgccggtt cttcgcgccc agccctcttt gtcgatcttt
     1141 acacccaacc cgccgagaag cttcaatgtc gtatgaatat cggccagact cgggactcgc
     1201 tggtaccggc agggttccgc cgtcagtaac gatgctatca gaattggcag cgcggcattt
     1261 ttcgaaccgc tgacggtgac ttcaccagtg agtctcttgc ccccttgcac gatgattttg
     1321 tccattagaa gctctaggct aattttgacg aggcagcgtg ggcgacaatc acgcgatctc
     1381 tatggccata atctttgtga accaacggcg ccgtatagtt cccggcgccg ctcaataagt
     1441 ccgcgaccgc tcgcgccata gcagcgccaa tttccatgat taatgcgccg ccgggcggca
     1501 aatgccgaaa tgcttgggct acgatgtcgc gataataatg gagtccatcc acaccgccgt
     1561 ccaatgcggt tcgtggctcc cattcgctaa cctccggcgc caaagcggcg aactcaccgc
     1621 tctcaacgta cggcggattg gagacgatta gatggaactc acgccctctg attgactcga
     1681 acaaattgct ggcacggaag tggactcgat ccgctacttg atgacgcgcg gcattgtcgc
     1741 gcgcaatctc gagcgccgcc cgtgagctat caatagccag tacttgcgcc cgcggcagct
     1801 cctttgcgag actgactgca atggcgccgc tgccggtgcc gatatcgaga attcgcaacg
     1861 gcaagtcgtt gtcgaaaatg ccggcgcatt gaagtgagat ctcaacgagc cgttcggttt
     1921 ccggcctcgg tatgaggaca tcgggcgtaa ccaggaaatc aagagaccag aactcctgac
     1981 gaccggtgat gtaggctacc ggctcgcggc gcaaacggcg gatcaaaaaa ttactaaacc
     2041 gttgtcgcgc gctagactcc ggttgctcat cgagaccgac gtaaaggcgg gattttttga
     2101 tgcgtaaggc gtgacacatc agcacctcgg catcaagacg cgccgactca attcctgcag
     2161 cttgaagtcg ccgggtaccg tcgacaaggt cgcaacggat tgttcgtggt cccagcaatg
     2221 aacctaatga ctggctgtcc gtctccatgg aagtcatcac ccggtggctt tcagcgcctc
     2281 cgcttgataa tgcgtgatca gtccatcgac aatttcatcg atttggcctt ccatgatacg
     2341 ttcgagctgg taaagggtca gattgatgcg atgatcggtc agccttcctt gagggaagtt
     2401 ataggtgcga atccgttcac tccggtcgcc gctgccaacc atcagctttc gcgttgcggc
     2461 aatctccgag cgctgttctt cctgtttttt ttccaatagc cgagccatca tgattttcat
     2521 ccctttggct ctgttcttat gttgagactt ctcatcctgg cacgacacca cgatgccgct
     2581 cgggatgtgc gtgatccgca ccgccgaatc ggtcgtgttg acactctgac caccgggccc
     2641 tgaagagcga aaaacatcga tgcggatatc cttcggatcg atatcgagtt ccacttcatc
     2701 cgcttccgca agcaccgcaa ccgtgactgc cgaggtgtgg atacgcccgg aaccctcagt
     2761 gacgggaact cgctgcacgc gatgaactcc gccctcgaac ttcagccggc tataggcacc
     2821 ctttccctca accatcacaa taatttcttt aaagccgccg agaccggtcg gactagaggt
     2881 caaagtttca acgcgccagc cgcgagattc agcgtagcgc gaatacatcc ggtaaagctc
     2941 gccggcaaag agcgcggctt cgtcgccacc cgtgccggcg cgaatttcga taataacgtt
     3001 tttgctgtcg ttcggatctt taggcaggat cagatacttg agacggtttt ccaactcttc
     3061 ctcacgttgt cgaagagcac tgacctcttc ttttgcgagc tctcttatgg cctcgtcatt
     3121 ttcttcaagg agttgacgat tctcctggat ctcctgctcg actttgcgcc attcacggta
     3181 ggagacgacg atctcctcca attcagacct ctccttggcc agctttgaat attcccgctg
     3241 caggttggct aggcccggat ccgacatcaa acgttccagc tc
//
LOCUS       DASULZ010000614         3504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_679763, whole genome shotgun sequence.
ACCESSION   DASULZ010000614 DASULZ010000000
VERSION     DASULZ010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679763"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VFY96_12660"
     CDS             complement(<1..663)
                     /locus_tag="VFY96_12660"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5607365.1"
                     /translation="MKVTVLDDYQRAFDSTEAIKRLREKAEVQIFTEKLPSDAALAEA
                     LRDSQAIIPVRERTKFTASVLEQLPRLEYISQTGNHVYHLDMEAATRLGIVVSLAPGG
                     NSTTELAFGLMLAVMRRIPQSDAAMRQGEWRLVLGHVLKGKTLGILGLGKIGTEVAAI
                     ARAFGMKVIAWGPTLTQERAAKSDATFLALEQVLKTADVISIHLALSDQSKNLLDEAR
                     LRL"
     gene            complement(660..1055)
                     /locus_tag="VFY96_12665"
     CDS             complement(660..1055)
                     /locus_tag="VFY96_12665"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5607366.1"
                     /translation="MPRKIIQPKSLNDPRPRYSQAILNQGGQLLFIAGQTASDQDGNV
                     VGRGDIKAQTEQVYANLKAVLKAAGGSIDDLVMTTTYIVDREYREGYNEVRQRQYKKN
                     PPTSTLVIVKGLAHPDYLIEIAGIAVITK"
     gene            complement(1144..2142)
                     /locus_tag="VFY96_12670"
     CDS             complement(1144..2142)
                     /locus_tag="VFY96_12670"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607367.1"
                     /translation="MKLVYTYLTLCLLIAGTFAGPASAQNLKKIRIGYPSLSFRQSNV
                     WVAKEAGLFNKYGLEVEPIFLRGGQIATQALAGGDPPIVNIGTVVQADLTGFDLVLVA
                     AVETTYDQIVFARPSLTKLEQLKGKRFGISGFGAATNYAALILMKHLNFQPKDLTLLP
                     TGPDAERLAAMSSGKIDATFFTSSSAAPARKAGFVELLQIADLGVEVQGNGFATSRAY
                     AQSNHDVVKNALKGFVEAIYYIYANKKEAQRVFAKYMRTNDPQVLEDSYNGYIHSIPK
                     KPYPTLKGIQFMLDMLAEKMPQAKNAKPEQFVDLSFLQELEKENFFNEMAKRYPTK"
     gene            complement(2184..3182)
                     /locus_tag="VFY96_12675"
     CDS             complement(2184..3182)
                     /locus_tag="VFY96_12675"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607368.1"
                     /translation="MRYLTRYLSLGIALLALTGVAQAADGGMLKMRFTQSGNTSSSWP
                     LYVAEQKKMFEKNGIQVEVIIIRGATNVVRAVLSETIPIGRINPDYVIGAADKGAKVK
                     IIAANMEKIPYDIYARPEIKTGADLKGKTLGVSTLTGGTTLMLHEVLDKAYKLKENDY
                     KLLVVGTSPERYAALKGGSVQASFMGPPFTFRAAKDGFRKLANFHEYLGPILFTTDFG
                     HANYLKSNRAEVVRYLKSMIEATRWLYEPKNKEEAISIHMKVLKSTRESAEDDYKYLV
                     QDFQPFPKDGLVSKVSYDKTMELRAKEGLYQGKKIPPLTEYVDNSFIEEALKQLGK"
     gene            complement(3207..>3504)
                     /locus_tag="VFY96_12680"
     CDS             complement(3207..>3504)
                     /locus_tag="VFY96_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5607369.1"
                     /translation="ALDAQTRNFMQLELLRIWRETKKTVIFVTHMISEAILLSDRVLV
                     FSHRPGTIRSEFSVPLERPRKMEMRSDRQFLELENVVWKQIEEEVNASGGFTQM"
BASE COUNT          745 a          933 c          938 g          888 t
ORIGIN      
        1 cagccgtaga cgcgcttcat caagcagatt cttgctttga tcggagagcg ccagatggat
       61 cgaaataaca tcagcggttt tgagcacctg ttcgagagcc agaaaggtgg cgtccgattt
      121 ggcggcgcgc tcttgcgtca aggtcgggcc ccaggcgatc actttcatgc cgaaagcgcg
      181 cgcgatggcg gcgacttccg taccaatttt ccctaagccc aaaatcccaa gagtttttcc
      241 cttgagcaca tgtccgagca cgagacgcca ctcgccctgg cgcatcgcgg catcgctctg
      301 aggaatgcgg cgcatcacgg caagcatcag accaaatgcc aattccgttg tgctgttgcc
      361 gccgggagcc aagctgacca cgatgcctag acgcgtcgcc gcttccatat cgaggtggta
      421 aacatgattg ccggtctggg aaatatattc tagacgcggc agctgttcta acaccgatgc
      481 agtgaatttg gtgcgctcgc ggaccggaat gattgcttgc gagtcgcgca gagcctcagc
      541 cagggcggca tcggatggca gcttctctgt aaaaatctga acttcggcct tttcgcgcaa
      601 ccgcttgatc gcctcggtcg agtcaaacgc ccgctggtaa tcgtcgagaa cggtgacttt
      661 cacttggtga tgacagcgat tcctgcaatt tcgatcagat aatccggatg cgcgagtccc
      721 ttgacgatca ccaaggtgct cgtcggcggg tttttcttgt actgccgttg acgcacctcg
      781 ttgtaacctt cgcggtactc gcggtctaca atgtaggtgg tggtcatcac gagatcatct
      841 atgctccccc cggcggcttt cagcaccgct ttgaggttgg cgtaaacctg ctcggtctgc
      901 gctttgatat cgcctctacc gacaacgttt ccgtcctgat ccgaagcggt ctgtccagca
      961 atgaacagca gttggccgcc ttggttgagg atcgcctggc tgtagcgcgg ccgagggtcg
     1021 ttgagggact ttggttgaat tatctttcgt ggcatagtcc tacctcctgg ttaagtcgtt
     1081 gcagtaatgc agggttggtt tcttttccga ctcaatactc catcactccg ttactccagt
     1141 gttttatttg gttggatacc gtttggccat ctcgttaaaa aagttttcct tctccaactc
     1201 ctgcagaaag cttaagtcaa cgaactgctc gggcttcgcg tttttcgcct gaggcatctt
     1261 ctccgccagc atgtcgagca tgaactgaat accttttagg gtcggatagg gttttttcgg
     1321 gatcgaatga atgtagccgt tgtaggaatc ctcgagcact tgcggatcgt tggtgcgcat
     1381 atacttggcg aagacccgct gggcttcctt tttgttggca taaatataat aaattgcttc
     1441 gacaaagccc ttgagagcat tcttgaccac atcgtgattg ctctgagcgt atgcgcgaga
     1501 tgttgcgaag ccgttaccct gaacttcgac accgaggtcg gcgatctgca gtagctcgac
     1561 aaaacccgcc ttgcgcgccg gggcagccga agacgaggtg aaaaaagttg catcgatttt
     1621 gccggacgac atagcggcga gccgctctgc atcgggcccg gtcggcaaaa gggtgagatc
     1681 cttcggctga aaattcaaat gtttcattag gatcaacgca gcatagtttg tcgcggcgcc
     1741 gaatccgctg atgccgaagc gcttgccctt cagttgctcg agcttggtga ggctggggcg
     1801 ggcaaaaacg atctgatcat aggtcgtttc gaccgcggcg acgagcacaa gatcaaatcc
     1861 ggttaaatcg gcttgaacga ccgtgccgat gttgacgatc ggtggatcac cgccggcaag
     1921 cgcctgggtg gcgatctggc cgccgcgcaa aaaaatcggc tccacttcca agccgtactt
     1981 attgaacaac ccagcttctt tcgccaccca gacgttggac tgccgaaagc tcaacgatgg
     2041 atagccgata cgaatcttct taagattttg cgctgatgcc ggacctgcga acgtgccggc
     2101 aatcagcaaa cagagcgtga gataagtgta aaccagtttc atcgatgaaa gcctttctat
     2161 cagtgtgatc ctgttttctt gctctatttg ccgagctgtt tcaatgcctc ttcaatgaac
     2221 gagttatcga catactcggt gagcggcggg attttcttgc cttgatagag gccttccttg
     2281 gcgcgaagtt ccatggtctt atcataggag actttgctga ccagcccatc cttcgggaac
     2341 ggttggaaat cctgcacgag gtacttgtaa tcatcctcag cgctctcgcg cgttgatttg
     2401 agcaccttca tgtggatcga aatggcttcc tctttgtttt tcggctcgta gagccaacgg
     2461 gtcgcctcga tcatcgattt cagataacgc acgacttccg cacggttcga tttgaggtag
     2521 ttggcatggc caaagtccgt ggtgaataaa attggaccga gatattcgtg gaagttggcg
     2581 agcttgcgaa aaccgtcttt ggccgcccga aaggtaaatg gcggccccat gaacgacgcc
     2641 tgcaccgagc cgcctttgag ggcagcatag cgttcgggcg aagtgccgac gaccaaaagc
     2701 ttataatcat tttctttgag cttgtaggct ttgtccaaaa cctcgtgcag catcagagtc
     2761 gttcccccgg tgagagtgct cacgccgagg gttttgcctt tcaaatcggc gccggttttg
     2821 atttcaggcc gggcgtaaat gtcgtaggga attttttcca tattggcggc gatgattttt
     2881 accttcgcgc ctttatccgc ggcgccaatc acgtaatcgg ggttgattcg gccgatggga
     2941 atggtctcgc tgagcacggc gcgcacgaca tttgtcgcac cgcggatgat gatcacttcg
     3001 acttggatgc cgttcttctc gaacattttt ttttgctcgg cgacgtagag cggccagctc
     3061 gatgacgtat tgccgctctg ggtgaagcgc attttcaaca ttccgccatc ggccgcctgc
     3121 gccacaccgg ttagggcgag cagcgcaatt cccaaactta agtatcgtgt caaatatctc
     3181 atcccaagac ctctttcttg ctgcaactac atctgagtaa acccgcccga ggcattcact
     3241 tcctcttcaa tttgtttcca gacgacattt tctaattcga gaaactgacg gtcggaccgc
     3301 atctccattt ttcttggacg ctccagcgga acagaaaatt cagatcggat cgtgcctgga
     3361 cgatggctga ataccagcac ccggtcggat aacaaaatcg cttcagagat catgtgggtg
     3421 acaaagatga cggttttttt ggtctcccgc cagatgcgca ataactcgag ctgcatgaaa
     3481 ttgcgagtct gcgcgtcgag cgcc
//
LOCUS       DASULZ010000615         3045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1491733, whole genome shotgun sequence.
ACCESSION   DASULZ010000615 DASULZ010000000
VERSION     DASULZ010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1491733"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VFY96_12685"
     CDS             complement(<1..321)
                     /locus_tag="VFY96_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5607370.1"
                     /translation="MPLNNLQSSLPSGSSAVIEFRKVNKWFGSLHVLKDIDLLVEPSK
                     VFVVCGPSGSGKSTLIRCINRLETFQSGSLTVDGLSLADGQLDPVRLRANVGMVFQSF
                     NLYPH"
     gene            435..893
                     /gene="sixA"
                     /locus_tag="VFY96_12690"
     CDS             435..893
                     /gene="sixA"
                     /locus_tag="VFY96_12690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HEX5607371.1"
                     /translation="MYAYLLRHGDAVAEMDNPKRPLSGKGRQNVYRTARLALRRNIQI
                     SAIYHSGILRALETAQILAEILAPPLGLHLHAGLLPQDDPAIVKVELDLMDHPIAWVG
                     HLPHLNRLAALLTSGDPKRAVVEFLPAMMVGFEKQNHQWKITWRSEEPAV"
     gene            950..2236
                     /locus_tag="VFY96_12695"
     CDS             950..2236
                     /locus_tag="VFY96_12695"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX5607372.1"
                     /translation="MASETLTIIDNRTGKQYEIPIKNGAVKATDLFQIRVTEDDGIVT
                     YDPGFMNTASCQSKITYIDGDKGILRYRGYPIEQLAEKSSYLETAYLILHGELPTSSQ
                     LNDWTHHITFHSIIHENIKKVIDSFHHEAHPMGVLISTVAALSTFYPEAKKIFDVKSR
                     RDQTYRLISKMPTLAAFAYRHSLGLPYSYPDNDLSYTGNFLNMLFKMTELKYKPNPVI
                     ERALDVLFILHADHEQNCSTNAMRGVGSSHVDPYSAVAAAAAALYGPLHGGANEAVLR
                     MLMEIGSKDHVPEFIKKVKGGEGRLMGFGHRVYKNYDPRAKIIKEVADQVFEVRGRNP
                     LLDIALELERIALSDDYFIKRKLYPNVDFYSGLIYQSMGLPMDMFPVLFAIPRTSGWI
                     AQWEEMLTDADQKLARPKQVYLGPQVRDYVPMTKRS"
     gene            complement(2707..>3045)
                     /locus_tag="VFY96_12700"
     CDS             complement(2707..>3045)
                     /locus_tag="VFY96_12700"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5607373.1"
                     /translation="PDQSDALEMFPDGKGEIEDEVRRYQMLRLSSRIGFKPPYFYNYA
                     LRNRLHRVSSPTQIIWGEHDRFVPRSHGETYAKLIPHAGMESVAGAGHSVPVEKPQET
                     AQLILDFLSA"
BASE COUNT          779 a          767 c          812 g          687 t
ORIGIN      
        1 gtgcggatag agattaaaag actggaagac catcccgaca ttggcgcgca accgtaccgg
       61 gtcgagttgt ccatccgcta aagacagtcc atcgacagta agagaaccgg attgaaaagt
      121 ctccagccga ttgatgcaac gtatcagagt acttttaccg ctgccgctcg gaccacaaac
      181 gacaaaaacc ttgctaggtt caaccaagag atcgatgtcc ttgaggacgt gaagcgagcc
      241 gaaccatttg ttcaccttac gaaactcgat cacagcggac gaaccggatg ggagagaaga
      301 ttgcaaattg ttgagcggca tactatatga acaacggttt ttttcaacta cggacagaac
      361 agggcgccga gcagcagcag ggccatggcg tcgccagatt cctcacctaa acttcaccag
      421 aggtaagccc gatcgtgtat gcatatctac ttcggcacgg cgatgctgtt gccgaaatgg
      481 ataatcctaa acgcccgctc tcggggaaag gacgacaaaa cgtttatcga acggctcgtt
      541 tggcgctccg tcgcaacatt caaatatcgg ccatttatca ttcaggaatt ttacgagcct
      601 tggagacggc gcagatactg gccgagattt tggcgccgcc gctcggcttg catttgcatg
      661 ctggcctatt accccaagat gatccggcga ttgtgaaagt cgagcttgac ctgatggatc
      721 atccgattgc atgggtgggt cacttgcccc atctcaatcg gcttgcggcg ttgctgacaa
      781 gcggcgatcc gaaacgtgct gtggtggagt ttcttccagc catgatggtc ggttttgaaa
      841 agcaaaatca ccaatggaaa attacttgga gatctgagga gccagcagtc tgacgcgtgg
      901 ttgaccggcg catagggata gtctaaaagg aaattgaaag gtaggcgcca tggcttcaga
      961 gactttgacg attattgata accggaccgg gaagcagtac gaaattccga ttaaaaatgg
     1021 cgcggtgaag gcgaccgacc tgttccaaat cagagtcact gaggacgatg gcatcgtcac
     1081 ttatgatccg ggattcatga acacggcttc gtgccagagc aaaatcacct acattgatgg
     1141 cgacaaaggg atcttacgtt accgcggtta tcccatcgag caactggctg aaaaaagttc
     1201 gtatttagaa accgcgtatt tgattcttca cggcgaactg ccgacctcat cacagctcaa
     1261 tgactggacc catcatatta cgtttcattc cattattcat gagaacataa aaaaagtaat
     1321 cgacagtttt catcatgagg cccatcccat gggcgtgctg atcagcacgg tggcggcgct
     1381 atccactttt tatccggagg ccaagaaaat ctttgacgtc aaatcgcggc gcgatcaaac
     1441 ttatcgtctg atcagcaaaa tgccgaccct ggccgcattc gcctatcgcc acagtctcgg
     1501 cctgccgtat tcttacccgg acaacgattt gagctatacc ggaaattttt tgaacatgct
     1561 gtttaaaatg acggagctca aatacaagcc gaatccggtg atcgagcgcg ccctcgatgt
     1621 gctgttcatt cttcatgcgg atcacgagca aaattgcagc accaacgcga tgcgtggtgt
     1681 cggcagttct cacgtcgatc cttattcggc agtcgcagct gccgctgccg ctctgtacgg
     1741 cccgttgcac ggcggcgcaa atgaagcggt gctgcgaatg ctgatggaaa tcggctcaaa
     1801 agatcatgtg cccgagttta ttaaaaaggt aaagggcggc gagggcagat tgatgggctt
     1861 tggccaccga gtgtataaaa attatgatcc gcgcgccaag atcatcaaag aggttgccga
     1921 tcaagtcttc gaagttaggg ggcgcaatcc cttgctggac atcgccctcg agcttgaacg
     1981 gatcgctctc tcagacgact actttatcaa gcgcaagctt tatcccaacg tggacttcta
     2041 ttcgggactg atttatcaat cgatggggct cccgatggat atgttcccgg tgctgtttgc
     2101 gattccgcgc acgtccggat ggatcgctca atgggaagag atgttgacgg atgccgatca
     2161 gaaactggcg cgtccgaaac aagtttatct cgggccgcaa gtacgcgatt acgtcccgat
     2221 gacaaagcgt tcctgaacta acgtagcgtg ccgcgaaacc cctgcaaaaa aatcatcaat
     2281 ctctgaccga ttcttaaacg cgccctcgag agctcgttca aattcatgag cgcgcggact
     2341 tcaagaccgt ggcggtgtgg cgctcgattg agcgcgcgga ttcgcacggg agttgtcgtt
     2401 gcctcggaag aattctgcgg cgtttccagg gttccagcaa ccactgcaac gtgagaaaaa
     2461 tcagcaaccg aataattgac gatccgactc ggaaaggccg gcacggtcgt gaattgatac
     2521 aaacgctcga aagaggcgca tccgattatt ccagtaactc ctaagaagag cgcaaatttt
     2581 actcgatcgc tcgcatagcg agtaacttag gaacgaggcg atacgtgaat aacgcgggcc
     2641 gcgcaagcga atccgacgag gaataggcta gaaccgattc tttcggttgt cgaaagtgcg
     2701 ggatgatcag gcggagagga agtctaatat caattgcgcc gtctcctgtg gtttttcaac
     2761 cggaacgcta tgaccggcgc ctgcgacact ttccattccc gcatgcggaa tgagcttcgc
     2821 atacgtctcg ccgtgggagc gcggcacgaa tctgtcgtgt tctccccaga taatttgcgt
     2881 tggagatgag acacgatgca gacgattgcg cagcgcgtaa ttgtaaaaat aaggcggttt
     2941 gaagccgatg cgcgagctga gccgaagcat ttggtagcgg cggacttcgt cttcgatctc
     3001 gcctttgccg tcgggaaaca tttccagggc atcggactga tcagg
//
LOCUS       DASULZ010000616         2892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_273819, whole genome shotgun sequence.
ACCESSION   DASULZ010000616 DASULZ010000000
VERSION     DASULZ010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273819"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..432
                     /locus_tag="VFY96_12705"
     CDS             145..432
                     /locus_tag="VFY96_12705"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEX5607374.1"
                     /translation="MVVGVLKLKLMIPENHSLKGKRGVLKSIQSRVGRQFNVAVTECG
                     DQDLWQSAILGFAAAGTTQPGVEKTLARVVDFVDSLGLAELSDSEMECFHC"
     gene            474..815
                     /gene="rbfA"
                     /locus_tag="VFY96_12710"
     CDS             474..815
                     /gene="rbfA"
                     /locus_tag="VFY96_12710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX5607375.1"
                     /translation="MDFQRSDRVGDLLLRALAELLAREIRDPRVRSINLTGVKLSKDL
                     RHARIFFSVFGAEANKSDALAGLQHATGFIRSKLGKELSLRFVPSIEFSYDDTEAEAQ
                     RIESLLRAAKP"
     gene            876..1775
                     /gene="truB"
                     /locus_tag="VFY96_12715"
     CDS             876..1775
                     /gene="truB"
                     /locus_tag="VFY96_12715"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX5607376.1"
                     /translation="MSRSGILLIDKAEGPSSAQIVHRVKNMLAAKKVGHLGTLDPFAS
                     GLLLVGVNEGTKIAEIFLSGTKRYHGIMVLGVETDTQDGTGKVLKELPVPAIMEKDLR
                     VVEKDFTGALQQVPPMFSALKRDGVRLYRLARQGKEVPREPRPIRIHELCVEKVNERE
                     LKFEVTCSRGTYVRTLAADMGRRLGCGAYLKALRRVGCDHLDVDRAIRVEDLVKIGSA
                     TEIPFVSLATAVNHLRAVVWQSRLVSRLRLGQQEVLGQIGPRQGCEEFVAILEPRGEL
                     AALAKWVDDIPGGRWRLFRVFRC"
     gene            1937..>2892
                     /locus_tag="VFY96_12720"
     CDS             1937..>2892
                     /locus_tag="VFY96_12720"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HEX5607377.1"
                     /translation="MSSSTGRVPSNSHIQRWVKDMAAMCQPDDIYWCDGSEEEKDSLT
                     SVALKSGDLIALNQEKLPGCYLHRSALNDVARTENLTYVCTEQREDAGPNNNWMAPAE
                     SYDKLAKIFSGAMRGRTLYVIPFLMGPASSSFSKVGIQITDSVYVVLNMRLMTRMGQI
                     ALDHLGDSDDFTRCLHSKADLDMERRFICHYPQDNTIWSVGSGYGGNALLAKKCLALR
                     IASKLGQKEGWLAEHMLIVGVENPEGEIHYICGAFPSACGKTNLAMLVPPPSMKGWKI
                     HTIGDDISWLRVGPDGRLWAINPEAGFFGVAPGTGSKTNPNA"
BASE COUNT          631 a          768 c          842 g          651 t
ORIGIN      
        1 ttccaggacc tcaagcaggg tgacgtcatc gaagcttacg agcgccttcc ggtggtgcgg
       61 cggatcaatc cgatgcccca cggccgcgag gcgccgcgcg tgagcggggt ataattcaga
      121 gttaatctcg accgtgttac ctccatggtt gtgggagtcc ttaagctcaa gctgatgatt
      181 cctgagaacc actcgctcaa gggcaaacgc ggcgtgctca aaagcattca gagtcgcgtt
      241 ggccgtcagt tcaatgtcgc agtgaccgag tgcggcgatc aggatctctg gcagagcgcg
      301 atcttgggat tcgctgctgc gggtacgacg cagccgggag tcgagaaaac cctggctcgc
      361 gtcgtcgatt ttgtcgattc cctcggactc gccgagctga gcgattccga aatggagtgt
      421 tttcattgct gagcttgaca gatactcaat gtgagaagag tttgttatag cgcatggact
      481 ttcaacgttc ggaccgggtt ggcgacctgt tgctgcgagc cttggcggag ctcctcgctc
      541 gagaaattcg cgatcctcgg gtgcgatcga taaacttaac cggcgtcaag ctgagcaaag
      601 acttacgcca cgcgcggatc tttttcagtg ttttcggcgc ggaggccaat aaaagcgacg
      661 ccctcgcagg tctccagcat gccaccggct tcatccggtc aaagctcggc aaagaactga
      721 gccttcggtt cgtgccgtcg attgaattta gctacgatga caccgaggct gaagcccagc
      781 gcatcgaatc actgctcaga gcggccaagc cttagtttcg ttcatttcct acccggcttt
      841 ccagttcatt caacaggtta attcgtggca tgcctatgag ccgaagcgga attttgctga
      901 tcgataaggc cgaaggccca tcctccgcac aaatcgtgca ccgggtcaaa aacatgctgg
      961 cggccaaaaa agtaggccat ctcggtactc tcgatccgtt cgcatcgggt cttctcttag
     1021 tcggcgtgaa cgagggaaca aagattgccg aaatttttct cagcggaacc aagcgctacc
     1081 acggcattat ggttctgggc gttgaaacgg acacccagga cggtaccggc aaagtgctca
     1141 aggaattgcc agtgccggcc atcatggaaa aagatttgcg agtggtagaa aaagatttta
     1201 ccggagcgct tcagcaagtg ccgccgatgt tctcggcgct caagagagac ggcgtacgtc
     1261 tatatcgatt ggcccggcaa ggcaaggaag ttccacgtga gccgcgtccg attcggatcc
     1321 atgagctgtg cgttgagaag gtgaacgaac gggaactaaa attcgaagtg acttgctccc
     1381 gcggaactta cgtgcggacg ctagcggcag acatgggtcg tcggctcggt tgcggcgcct
     1441 atctcaaagc gcttcggcgc gtcggctgcg atcatctcga tgtcgatcga gccattcgcg
     1501 tcgaggatct ggtcaaaatc ggatccgcaa cagagattcc gttcgtttcg ctagcgaccg
     1561 ccgtgaatca cctgcgcgcg gtcgtttggc agagccggct ggtgtcgcgc ttgcgtttgg
     1621 gacagcaaga agtcttgggg caaatcggtc cgcggcaagg ttgtgaggag ttcgtcgcga
     1681 ttctcgagcc gcgcggcgag ctcgcggcat tggctaaatg ggtggacgat atacccggcg
     1741 gccggtggcg tcttttccgc gtgtttcgtt gctagcgcga atattgacat cgccgcggct
     1801 catcggatag ggttctgttg taaaacgcgt tgccgctctg gcgcgcattt tttttcgcga
     1861 cgacatgatt gtcccgctcg ctccacgaga accccgcgag atgcaagatc taacgaaagg
     1921 aaacattggc taacccatga gctcctcgac aggtagggtc ccgagcaatt cgcatattca
     1981 aagatgggta aaggacatgg ctgccatgtg ccagccggat gatatttatt ggtgcgatgg
     2041 gtcggaagaa gagaaggaca gtttgacgtc cgtcgcgctc aagtccggcg atctgattgc
     2101 tctgaatcaa gaaaaattac caggctgcta tcttcatcgg agcgcgctca atgacgtcgc
     2161 gcgcaccgag aatctgactt acgtgtgcac tgaacagcgc gaagacgcag ggccgaacaa
     2221 taactggatg gcacctgctg agtcgtacga taaattggcc aagatcttct ccggcgcgat
     2281 gcgcgggcgc accctttacg tcattccttt cctcatgggt ccagcaagct cttcgttcag
     2341 caaagtaggt atccagatca ccgatagcgt ctacgtcgtt ctcaatatgc gtttaatgac
     2401 ccgcatgggg caaatcgctt tggatcacct cggtgattca gatgatttca cgcgctgtct
     2461 tcattccaag gccgatctgg acatggagcg tcggtttatc tgccattatc cccaagacaa
     2521 cacgatctgg agcgtcggct caggctacgg tggcaacgct ttgttggcca agaaatgtct
     2581 tgctctgcgg attgcgagca agctcggcca gaaagagggc tggctcgccg agcatatgct
     2641 gatcgtgggc gtggaaaacc ccgaaggcga gatccattat atttgtggcg cgtttccgag
     2701 cgcctgcggc aaaaccaatc tcgccatgct ggtaccgcca ccgagtatga aaggctggaa
     2761 aattcatacc attggcgatg atatttcgtg gctccgggtt gggcccgacg gacggctctg
     2821 ggcgatcaac cctgaagctg gattttttgg cgttgctccg ggcaccggtt ctaaaaccaa
     2881 tcccaatgcg at
//
LOCUS       DASULZ010000617         2675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1187698, whole genome shotgun sequence.
ACCESSION   DASULZ010000617 DASULZ010000000
VERSION     DASULZ010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187698"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="VFY96_12725"
     CDS             complement(<1..969)
                     /locus_tag="VFY96_12725"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5607378.1"
                     /translation="MAEKFSVIAKNGMVASSQPLATLAGVQILTNGGNAIDAAIATAA
                     VLGVVEPSSIGIGSDAFCLFYSAKEKKIKALDASGRSPYAATLEFCRNRGFTEMPQRG
                     IHSVTVPGAVHGWATLLNTFGKKKLADVLQPAIDHAEEGFPVAELTAEQWKESEARLK
                     ADQGAAMNYLINGGTPKAGEIFRNPRMAAVLKRIAAEGPEYFYRGDIAKKLVQCSERL
                     GGVFKLRDFAEHHSDWIEPITVSYRGYDIYEMPPATQGFVALEMLKILEGFDLKAAGA
                     QSADALHFMIEAKKLAFADRDAHLADRDYMKVPVESLFSSKRIEALR"
     gene            complement(1127..2140)
                     /locus_tag="VFY96_12730"
     CDS             complement(1127..2140)
                     /locus_tag="VFY96_12730"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5607379.1"
                     /translation="MKRMRYMLLSLIAAVSMPVCGHSQENQTQKIEAARKEGEVVWYT
                     TMSSDQSNAFMARFQQKYPFLKPAVIRLGGSALLNRILTEAKAGKNFFDVAHGTGEIV
                     LPLMEMGLLAPYLSPERKMIPDDLKDKKGFWTSVYVNSVVLGYNTNLVKGQPIPRTYS
                     DLLQPRWKGRKISVDDTYATFLQGLISAWGKDKALDYLKKLAEQDPVVMRGSTVRVQL
                     AAAGEFPLVIAYANIIQYLAEKGAPVDWVALEPAVISVNTVMASAKAMHPNAARLFID
                     FTLSKEGQEKLWDFQRIPSRSDVEPKPARLFRGYKRYVVHPEEMQNLDETAKLYSQIL
                     KTR"
     gene            complement(2234..2587)
                     /locus_tag="VFY96_12735"
     CDS             complement(2234..2587)
                     /locus_tag="VFY96_12735"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5607380.1"
                     /translation="MRPKVRHVAVMTKNREKMVDFYQKIFGLEPKRGFGGAIYMSDGD
                     VNIALIEVKREDQKEGINHFGFEVDDLDEIKRRLQEAGFNSEIDSKSDKDSDHRVQDP
                     DGHWVDMAVHRRWPK"
BASE COUNT          586 a          786 c          664 g          639 t
ORIGIN      
        1 gcgcagcgct tcgatccgct ttgaggagaa aagactttcc accggaacct tcatataatc
       61 gcgatccgcg agatgggcgt cgcggtcggc aaatgcgagc tttttcgcct caatcatgaa
      121 atgcagagca tccgcacttt gcgcgccagc ggctttcaaa tcgaagcctt cgaggatctt
      181 gagcatctcc agcgctacga agccttgcgt ggccggcggc atctcgtaaa tatcatatcc
      241 acggtaggac acggtgatcg gttcgatcca gtcggagtgg tgctcggcga agtctcgaag
      301 cttgaaaact ccgccgagcc tttctgaaca ctgaacgagt tttttggcga tgtcgccccg
      361 gtaaaaatac tccggccctt ctgccgcgat gcgcttgagc accgccgcca tgcggggatt
      421 tctgaagatt tcgccggctt tgggcgtccc gccgttgatc agataattca tcgccgcgcc
      481 ttgatcggct ttcaatcgcg cttcggattc tttccactgt tccgccgtca gctccgccac
      541 gggaaagcct tcctcggcat gatcgatcgc cggttgcagc acatcggcga gttttttctt
      601 gccgaaggta ttcaacaaag ttgcccaacc atgcacagcg ccgggaaccg taaccgagtg
      661 aatgccgcgc tgcggcatct ctgtaaaacc cctattgcgg caaaactcca aggttgctgc
      721 gtagggtgat ctgccgctgg catcgagcgc tttgatcttc ttttctttgg ccgaataaaa
      781 aagacagaac gcatcactgc cgatgccaat tgacgaaggc tcaacgacac ccagcacggc
      841 agccgttgcg atcgctgcat cgatcgcgtt tccgccattc gtgaggattt gcacgcccgc
      901 caaggtcgcc agcggctggc tagacgccac catgccgttt tttgcgatta ccgaaaactt
      961 ttctgccatg agagcaccac tcccgattta ttcaaacaaa tgatccaacg cgtttcttca
     1021 gtccggccgc tctgaaccgg gaatcttccg gacgccgggc gcagcatact ggggccccta
     1081 caggtcgcct ggtgccctct tgcctgattc aggaggccta gcgcagttaa cgcgttttga
     1141 gaatctgcga atagagcttc gcggtctcat cgagattctg catctcttcc ggatggacga
     1201 catagcgctt gtagccgcga aacagccgtg cgggtttcgg ctccacatcg ctgcgtgagg
     1261 ggattcgctg gaaatcccaa agtttttcct gtccctcttt ggaaagcgta aagtcgataa
     1321 acagtctcgc cgcgttcggg tgcatcgcct tagcgctcgc catcaccgtg ttgacggaaa
     1381 taacggctgg ctcgagcgcc acccaatcca ccggcgcgcc cttttcagcg agatattgga
     1441 tgatattggc gtaagcgatc acgagaggaa actcgcccgc cgccgcgagc tgaacgcgca
     1501 ccgtgctgcc gcgcatgacc acgggatctt gctctgccag ttttttcaga taatcgagtg
     1561 ccttgtcttt gccccatgcg gagatcagtc cttgaagaaa cgtcgcataa gtgtcatcca
     1621 cagaaatttt tcggcctttc caacgcggct gcaacagatc gctatacgtg cgcggaatcg
     1681 gttgtccttt gacgagattg gtattgtacc caagcaccac ggaattgacg taaaccgatg
     1741 tccagaaacc tttcttgtct tttaaatcgt caggtatcat tttgcgctcg ggcgaaagat
     1801 acggcgccaa aagccccatc tccatcagag gcaagacaat ctcgccggtg ccgtgggcga
     1861 cgtcaaaaaa gtttttaccg gctttcgctt cggtcagaat tcggttcaac aatgcgctcc
     1921 caccgaggcg gatcacggcg ggcttgagaa atggatactt ctgttgaaaa cgcgccatga
     1981 aggcgttgct ctgatccgag ctcatggtgg tataccaaac cacttcgcct tccttgcgcg
     2041 ccgcttcgat tttctgcgtt tgattttctt gagaatgtcc acagacgggc atcgagaccg
     2101 ctgcaatcaa actcagcagc atgtatcgca ttcgcttcat gacaaccgaa cttcagcgct
     2161 catgcgctca catttcacaa ctattacgcc cctcacgcct cactccttgc cccttacggc
     2221 tttcaattca ctgctacttc ggccaacggc ggtgaaccgc catatcgacc cagtgcccgt
     2281 caggatcttg gacgcgatga tcggagtctt tgtccgattt cgagtcgatc tccgagttga
     2341 aacccgcttc ctgcaagcgg cgcttgatct cgtcgagatc gtccacttca aaaccgaagt
     2401 gattgatgcc ttccttttga tcctcgcgct tgacttcgat gagcgcgata ttgacatcac
     2461 cgtcggacat gtagatcgcg ccgccaaaac cgcgcttagg ctcgaggccg aagattttct
     2521 ggtaaaaatc caccattttt tcgcggttct ttgtcatcac cgcaacgtga cgcaccttgg
     2581 gtcgcatttc gaaaacctcc tttcaatcat taactgctca aagcaaatgc gaaatccgaa
     2641 tatcgaaatc cgaaaaaaac gacaaaccga aaacc
//
LOCUS       DASULZ010000618         4558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1288711, whole genome shotgun sequence.
ACCESSION   DASULZ010000618 DASULZ010000000
VERSION     DASULZ010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288711"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(244..1290)
                     /locus_tag="VFY96_12740"
     CDS             complement(244..1290)
                     /locus_tag="VFY96_12740"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607381.1"
                     /translation="MRKRTLLAVGMTALVFVLVAGNRSETRAQQKELPEISMLTAVPN
                     FAFAAIWVAEQLKYFEQEGIRVKITPAPSGSVCLNAVVGRSTNFCASTSEGLVLARAE
                     GAPAMAIQAHNRAMTLSVVLRKAIVDKLGLTRESPIGARLKALTQLGTIGATGPGAAS
                     EQIFKFLVKKAGAGGTVLKFVYIGAPELPASLMNNVIDAYALSPPSAEVTEPSGKGYV
                     LIPLGKGEVPELTDYPYEVLMVRPDYVEANPKIAMAVSRAISRGGALFRSKPEEAKAA
                     LRAHRLGVKLDENVFELSYNMVAGAMPAWGNMSPAGWQKVINFATGAGIVKEAQAPSN
                     KEGVLWTNKYVGKP"
     gene            complement(1305..2165)
                     /locus_tag="VFY96_12745"
     CDS             complement(1305..2165)
                     /locus_tag="VFY96_12745"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5607382.1"
                     /translation="MSDPTVQARSRPAGSKTTVRAVRPAIVSMGTMGVNLARITIIGG
                     FLLLWEIASGRWIEPFLVSSPSRIFFSLISGYRDGDLLQHTWVTFQEIAIGFPIGAIS
                     GIALGYGFGRSRLLAEIFEPIIIALNGIPRTALAPLFIVWLGIGLWSKVGVVFLLTFF
                     LNFFNTYTGMRQMDQEYVDLANLMGVKGWKLSWKVILPAISPYVFTGIRTSIPFAVIG
                     AIVGEFVAATEGVGFFIRMSAGIFKTADVFVGIIVLMIMVIIMDRIAELIERRALRWQ
                     SQTEHIQIQA"
     gene            complement(2179..3006)
                     /locus_tag="VFY96_12750"
     CDS             complement(2179..3006)
                     /locus_tag="VFY96_12750"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5607383.1"
                     /translation="MPDLTRNPGQVAPLRSDAVIRVEKVSKVFHTPSRENLDALIDIE
                     LSIHDGEFITVVGPSGCGKSTLLRLIAGFSPCSSGRIMFQGEEVRGLNTKVGYVPQES
                     KLFPWLTVEENVGFGLDSRQYSRADRDRQVGQFIHLAGLAGFEKYYPGQLSGGMSKRA
                     SIVRALAYEPPVILMDEPFGPLDAQTRMILQDELLKIWEKKRQTIIFVTHDLVEAVAL
                     ADQVVVMTHRPGKIRDVVRVPMSRPRNIFEIHRQDGFDEAYGRLWNIFRHELSISAH"
     gene            3292..4401
                     /locus_tag="VFY96_12755"
     CDS             3292..4401
                     /locus_tag="VFY96_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX5607384.1"
                     /translation="MSRSLIDHRGPEFATLLKDVIEGLKGVFKTSEGRIAVFPGSGSA
                     AWEACIVNTLSPGDRVLGCVNGHFSNLFCRTAESFGIQVEALEFPYGEGVSGARVEER
                     LQRDVKQDIKAVLVVHNETSTGVTSDIPAIRHALDRACHPALLLVDSVSSVASIEFRF
                     DDWRVDVALAGAQKGLMLPPGVAILAIAERALEASKSARCPRSFWDWVPILERNRLGE
                     VPSTPVTAHFFGLKESLAMLGEEGLPNVFRRHARLAESCRRAVQAMGLRLLAGNPAEY
                     SNTLTAVRMPEGFDADAFVAHAYKQLNFSLGIGLGQVKGKIFRIGHLGSLNELELLGA
                     LAGVEMALKSFGVRIPLGAGLAAAENYLLETAVDR"
     gene            4413..>4558
                     /locus_tag="VFY96_12760"
     CDS             4413..>4558
                     /locus_tag="VFY96_12760"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX5607385.1"
                     /translation="MKIDPDKCVACGNCVPICPMGAISIDPVIGRATVNADECVECFT
                     CYRG"
BASE COUNT          987 a         1258 c         1274 g         1039 t
ORIGIN      
        1 tacaagagag gcgccggcag gtttcttaca ttttgcaaat ggaagttctt tttggcttgt
       61 cacctagtct cccgtgttaa ggcactgtca caatgtcatc ctgagtcgta gccgaaggat
      121 cttatttcta agcgcacgaa acacgagatt cttcgccttc gggctcatct atgaaagctt
      181 tgtgttctcg cattaccgac agcctcgaac tgacagtcga acgtaaaatc gctggtaagc
      241 cggctacggt ttaccaacgt atttgttggt ccagagcacg ccttctttat ttgatggcgc
      301 ctgggcttcc ttcactatgc ccgccccggt cgcgaaattg atgacttttt gccaaccagc
      361 ggggctcatg tttccccagg cgggcatggc gccggccacc atgttgtaag acagttcgaa
      421 aacgttctcg tccaatttga cgccgagtcg gtgagcgcgg agcgcggcct tcgcctcttc
      481 gggtttgctg cgaaacagcg cgccaccgcg cgagattgct cgcgacacgg ccatggcgat
      541 cttcgggttg gcctcgacgt aatccgggcg caccatcagg acttcatacg ggtaatcggt
      601 gagctccggc acttcgcctt tgccgagggg gatcaagacg tagcccttgc cgctcggctc
      661 ggtgacttcg gcggacggcg gagaaagcgc ataagcatcg attacattat tcattaatga
      721 ggcgggcagc tcgggcgcgc cgatgtagac gaatttgagc acggtcccac ccgccccggc
      781 ttttttcaca aggaacttaa aaatttgctc ggatgcggct ccgggtccgg ttgctccgat
      841 agtacccagt tgagtgagcg ccttcagacg tgcaccgatg ggactttcac gagtaagtcc
      901 cagtttatcg acgatggctt tgcgtaatac gacactcagt gtcatcgcgc ggttgtgcgc
      961 ttggatcgcc atcgctggag cgccttcggc tcgcgccagc accagtcctt cagaagtgga
     1021 ggcgcaaaag ttcgttgacc gaccgactac cgcgttcaag caaaccgagc cgctcggcgc
     1081 cggcgttatc tttacccgaa tgccttcctg ctcaaaatat ttgagctgct cggcgaccca
     1141 aatcgctgcg aaagcgaaat tgggcaccgc agtcaacatt gagatttcgg gaagctcctt
     1201 ttgctgcgct ctcgtttccg atctgtttcc agcgacgagc acaaaaacca gcgcggtcat
     1261 tccgaccgcc aagagagttc gttttcgcat ccgagaatct cctcctaggc ttgaatttga
     1321 atgtgttcgg tttgcgattg ccagcgtaat gcccggcgtt cgatcaattc agcgatacgg
     1381 tccatgatga tgaccatgat catcaaaacg atgattccga cgaaaacgtc ggcggttttg
     1441 aaaattcccg ccgacatacg gatgaaaaag ccgacgcctt cagtggcagc gacaaattct
     1501 ccgacgatgg cgccgatcac tgcaaacgga atgctcgtac gaattccggt aaagacgtag
     1561 ggagaaattg ccgggaggat cactttccaa ctcagtttcc atccctttac gcccatcaaa
     1621 ttcgccagat cgacgtattc ctgatccatc tggcgcatgc cggtataggt attaaaaaaa
     1681 ttgagaaaga aggtgagcag aaaaaccaca ccgactttcg accagagccc gatccccagc
     1741 caaacgataa aaagcggcgc caaggcggtg cgcggaatgc cattcagagc aatgataatc
     1801 ggttcgaata tttccgcgag caatcggctt cgcccgaacc cgtacccgag agcaatgccg
     1861 gaaattgccc cgatcggaaa tccgatggcg atctcttgga atgtgaccca ggtgtgctga
     1921 agcaagtcac cgtcgcgata accgctgatg agtgagaaga aaatgcggct tgggctactg
     1981 actaggaacg gttcgatcca acgtccggaa gcaatctccc aaagcagcag aaaaccgccg
     2041 atgatagtga tgcgagctag attcacgccc atagtgccca tggaaacaat ggcgggtctc
     2101 accgcccgga cggtggtttt tgaaccggct ggtcgggacc gcgcttgaac ggttggatcg
     2161 gacataaaaa tcaacctgct agtgcgcaga aatgctgagc tcgtggcgga aaatattcca
     2221 taaccggccg taagcctcgt cgaaaccatc ttgccggtga atctcaaaaa tattgcgcgg
     2281 tcgagacatc ggcacccgaa cgacatctct gattttcccg ggccggtgag tcatcacgac
     2341 gacctgatct gccagcgcca cggcttccac caggtcgtga gtgacaaaga tgatcgtctg
     2401 gcgttttttc tcccagatct tcaacagctc gtcttgcagt atcatgcgtg tctgtgcgtc
     2461 caacgggccg aagggttcgt ccatgaggat gacgggcggt tcataggcta gagcgcgcac
     2521 aatcgatgca cgtttactca taccgcctga gagttgcccg ggatagtatt tttcaaaacc
     2581 agcgagccca gccaaatgaa tgaactggcc cacctgccgg tctcgatcgg cgcgcgagta
     2641 ttgcctggag tctaggccaa agccaacatt ttcttcgacg gtcagccagg ggaacagctt
     2701 gctttcctgc ggcacgtacc cgaccttggt gttgaggccg cggacttctt ccccttgaaa
     2761 catgatccgt cccgaagagc agggtgaaaa tccggcgatg agtctgagca acgtcgactt
     2821 gccgcaaccg ctcgggccga cgacggttat aaattctccg tcatgaatgc tcagttcgat
     2881 atcgataagt gcgtcgagat tttcccggct cggcgtgtga aagaccttcg agactttttc
     2941 gacccggatc acggcatcgc ttcggagtgg cgcgacttga ccagggtttc gcgtgagatc
     3001 aggcatgcgt gggaactcgc ttgctgcggt atttatccat gcgaaacggg tagagtcaac
     3061 caagccggca ggtactttat gcggcttgac ttggctggtt gatgaacgtt acgctcatgc
     3121 acctttttac tcgcagtgat atacgcgatg cgacgtcgac cacgctgaaa catttttagg
     3181 ggacgatgcg aagcgccaat gactttacaa agtaaattat caggcaactc tggtcgcccg
     3241 tttctgcaaa ttcccggtcc gaccttggtt cccgaaagaa tcgttcgggc catgtcgcga
     3301 tccctgatcg atcaccgggg accggagttt gccacgctct taaaggatgt gatcgagggc
     3361 ttgaagggcg ttttcaaaac ctccgaagga cgcattgctg tatttccggg ctcgggcagt
     3421 gcggcgtggg aagcctgtat cgtcaacaca ctgtctccgg gtgatcgtgt gctgggctgt
     3481 gtgaacggtc acttcagcaa ccttttctgc cgcaccgccg aatcgttcgg catccaggta
     3541 gaagcgctag agttccccta cggcgagggt gtttctggcg cgcgagtcga agagcggctt
     3601 cagcgtgatg ttaaacagga catcaaagcg gtacttgtgg ttcacaatga gacttcgacc
     3661 ggtgtgacta gcgatatccc ggcgatccgc cacgcgctcg atcgcgcgtg ccacccggcg
     3721 cttcttttgg tcgactcggt cagctccgtg gcgtcgattg aatttcgctt cgacgattgg
     3781 cgcgtggatg tcgctctcgc cggcgcacag aaagggttga tgttgccgcc gggggtcgca
     3841 attctcgcca tcgccgagcg cgccctcgaa gcgagcaagt cggcgcgctg cccccgttcg
     3901 ttctgggact gggtgccgat attggagcgc aatcgtcttg gcgaagtacc ttccaccccc
     3961 gtcacggcgc acttttttgg cctcaaggaa agtctcgcga tgctcggcga agagggcttg
     4021 cccaacgtct ttcgccgcca cgctcgattg gcggaaagct gtcggcgcgc ggtccaggcg
     4081 atgggtttgc gccttctcgc tggcaatccc gccgaatatt ccaatacgtt gaccgccgtc
     4141 cgtatgcctg aaggatttga tgccgatgcg ttcgtcgccc atgcgtacaa gcaactgaat
     4201 ttttctctag gcatcggact aggtcaggtg aaaggcaaaa tttttcgcat cggccacctg
     4261 ggaagcctga atgagctcga gctcctcggc gcgctcgccg gggtcgagat ggcgctaaaa
     4321 agttttggtg ttagaattcc cttgggcgct ggcctggctg ccgcggaaaa ttatctgttg
     4381 gagaccgcgg tcgaccgtta aggtagaacg agatgaaaat cgatcctgac aaatgcgtgg
     4441 cctgtggcaa ctgcgtgcca atttgcccca tgggagcgat ctccatcgat cccgtgattg
     4501 gacgagcgac cgtcaatgcc gatgaatgcg tggaatgttt cacctgttat cgcggcat
//
LOCUS       DASULZ010000619         2371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_72101, whole genome shotgun sequence.
ACCESSION   DASULZ010000619 DASULZ010000000
VERSION     DASULZ010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72101"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1331
                     /gene="carB"
                     /locus_tag="VFY96_12765"
     CDS             <1..1331
                     /gene="carB"
                     /locus_tag="VFY96_12765"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX5607386.1"
                     /translation="PDGVIVQFGGQTPLKLALPLERAGVPIIGTSPDSIDLAEDRERF
                     ARLLKQLEIQQPDNGIARSYAAAFKIAERLGYPVLVRPSYVLGGRAMQIVYDDENLRD
                     YMTYAVSASPEHPVLIDKFLDDAIEIDVDALGDGEQVVIGGIMEHIERAGVHSGDSAC
                     SLPPKSISREVQDEIRRYTVALANALNVRGLMNVQFAVQRTRVYVLEVNPRASRTVPF
                     VSKAIGVPLAKIAARIMVGEKLADIGFVREIQPKHIAVKEAVFPFNKFPGVDTLLGPE
                     MKSTGEVMGIDTSFGLAFAKAQIGGGMRLPLSGRVFISVRDEDKPGVTPAAEKLHRAG
                     FIIVATAGTARYFAARGIPSEIVKKVQEGSPHIADQIKEGKVAMVINTPTDAHSHADS
                     FLIRRCALDYQIPYFTTIAGAEAAAEGIEYLKERDFDVKALQDYAAASLA"
     gene            1339..>2371
                     /locus_tag="VFY96_12770"
     CDS             1339..>2371
                     /locus_tag="VFY96_12770"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX5607387.1"
                     /translation="MKVAGSVPGVQSSAANAPPSVAASLRALGTSIRNLKGIGPKRAT
                     QLESFGIQTVLDLLRHLPFRYEDRRRIEKISSARPGMEATFVGRLIGLQKRYVPRRRS
                     QMLLATLQDDSGSIDLVWYRAPGYLADNLANGRMISAHGKIEPGMPGRLRMVHPEFDF
                     IESGQEAELQRILPIYVRPAGLPLSLMRRWIAQILSEYGVYLIDSLPQSITARHDLMT
                     LATAITELHAPPRDIDVDAFNSFSSRAHRTVVFEELFYLQLGLGERKRSRSQNRSIRF
                     SADKARLSQRMRDRLRFELTGAQRRALTEIETDMAQPQPMQRLLQGDVGSGKTIIAWF
                     ASLRAIEHGY"
BASE COUNT          504 a          686 c          701 g          480 t
ORIGIN      
        1 agcccgacgg ggtcatcgtt caattcggcg gtcaaacccc gctgaagctg gccttgccgt
       61 tggagcgggc cggcgtgccg atcatcggca cgtcgccgga cagtatcgat ctcgcggaag
      121 atcgcgagcg cttcgcgcgg ctgttaaaac agctcgagat ccagcagccg gacaacggca
      181 ttgcgcgctc gtacgcggcg gcttttaaaa tcgccgagcg actcggttat ccggttttag
      241 tccgaccctc gtacgtcctc ggcggacggg ccatgcagat cgtctatgac gatgaaaact
      301 taagggatta catgacctat gcggtttccg catcacccga gcatccggtc ctgatcgaca
      361 agtttctgga cgatgcgatc gaaatcgatg tggacgctct tggggatggc gagcaggtcg
      421 taatcggcgg catcatggag cacatcgagc gcgccggcgt gcactcgggc gatagcgcat
      481 gcagtttgcc gccgaagtcc atcagccgcg aagtgcaaga tgaaattcgt cgctatacgg
      541 tggctttggc aaacgcgctc aacgtgcgcg gtctgatgaa cgtgcaattc gccgtgcaga
      601 ggacgagagt ctatgttctc gaggtgaacc cccgagcatc acggacagtg cctttcgtga
      661 gcaaagcgat tggcgtcccg ctggcgaaga tcgccgctcg catcatggtt ggggaaaaac
      721 tcgcggacat aggcttcgtg cgggaaattc agcctaagca tatcgcggtc aaagaagcgg
      781 tttttccctt caataaattt cccggggtcg atacgctgct cggtcccgag atgaagtcga
      841 cgggtgaggt gatgggcatt gacacttcct tcggtctagc ctttgccaaa gctcagatcg
      901 gcggcggcat gcgccttccc cttagcggcc gggtcttcat cagcgtgcgc gatgaggaca
      961 agccgggggt tacgccagcg gcggaaaaac tgcaccgcgc tgggtttatc atcgtcgcga
     1021 ctgccgggac cgcgcgctac ttcgccgccc gcggaattcc ttcagagatc gtcaagaaag
     1081 tgcaggaagg aagtccgcat atcgccgacc aaatcaaaga aggcaaagtc gcgatggtga
     1141 tcaatacgcc caccgacgcg cattcccatg cggactcctt tctcattcgc cgctgcgcgc
     1201 tcgattatca gataccgtac tttacgacca tcgccggcgc cgaagctgcc gcagaaggaa
     1261 tcgaatatct gaaagagcgg gacttcgatg tcaaagccct gcaagactat gccgccgcaa
     1321 gcctagccta agccggacat gaaggtcgcc ggcagcgttc caggcgtgca atcatctgcg
     1381 gccaacgcgc cgcctagtgt cgccgcctcg ctgcgtgcgc tgggtacatc gatccgcaac
     1441 ctcaagggca taggaccaaa acgggcgaca cagctggagt cgtttggcat ccaaaccgtt
     1501 ttggatctgc tgcgtcatct gccgtttcgt tacgaggacc gccgccgaat tgaaaagatt
     1561 tccagcgcca gacccggcat ggaagcgacc tttgtcggcc gattgattgg cttacaaaag
     1621 cgctatgtcc cgaggcgccg aagccaaatg ttactggcaa cactccagga cgattccggg
     1681 agcattgatt tggtctggta tcgcgcaccg gggtatcttg ccgataatct cgctaatggg
     1741 cggatgattt cggcccatgg caagatcgaa cctggaatgc caggccgatt gcgaatggtt
     1801 cacccggagt tcgacttcat cgaatccggc caagaggcag agctgcaacg gatcttgccc
     1861 atttacgttc gtccagcggg gctgccgttg tccctaatga ggcgctggat cgcgcagata
     1921 ttgagcgaat acggtgtcta cctgatcgat tcgcttcccc agtcgattac cgcgcgccat
     1981 gatctgatga cacttgctac ggcaatcact gaattgcacg cgccgccgcg cgatatcgat
     2041 gtcgatgctt tcaacagctt tagttctcgg gcgcaccgca cggtagtgtt cgaggaactt
     2101 ttctatttgc agttgggatt gggtgagcgc aaacgctcgc ggagccaaaa caggagcatc
     2161 cgcttcagcg ccgacaaggc cagactcagt cagcgtatgc gcgaccggct gcggttcgag
     2221 ctcaccggcg cacagcgccg cgcgctgacc gaaatcgaaa ccgatatggc gcagccccaa
     2281 ccgatgcagc ggctgctgca gggcgatgtc ggatcgggaa aaactatcat cgcctggttt
     2341 gcgtctttgc gcgccataga gcacggctat c
//
LOCUS       DASULZ010000620         2754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_477177, whole genome shotgun sequence.
ACCESSION   DASULZ010000620 DASULZ010000000
VERSION     DASULZ010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477177"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VFY96_12775"
     CDS             complement(<1..452)
                     /locus_tag="VFY96_12775"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607388.1"
                     /translation="MRIHHRLSIVALLSVTLLAAQLDAQEKRNIRMVFVSLAWNSEIP
                     FRAALARGYFRQQGLQIEPILIRGGPAAIAALVSGEVDYASIGGAQAIFRGKARGLDL
                     SIIGCISSTTNYILLGNKQTRTVEELRGKPIGITGAGTYSEFAVKAFL"
     gene            749..1960
                     /locus_tag="VFY96_12780"
     CDS             749..1960
                     /locus_tag="VFY96_12780"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5607389.1"
                     /translation="MAESNSNRKLKLGIIGLGVGASEILPAMEQIPEFRLVAAADINA
                     RVLDIFRQRYGAKTYDSIEKLCADPEVEAVWVSTPNRFHAPHTIIAANAGKHVVVEKP
                     MAISLQEAEQMIEASVKNKIKLLCGHTQSFGPHIRTMRKIIRSGELGKLCALHVWAYT
                     DWMLRPRTAEELDIKQGGGVPFRQGPHQIDTLRLLGGGLVRSVRGATGQWFKGRPIPG
                     YYSGFLEFEDGTPATLMHNGYGYFLASELVPWGGQNSRYGEAERAGVRAALLNGTRDE
                     NADKDAMRIGGANEREIRDRSKAKPWLPNDLGILIASCEKGDIRQSQHGLFVHSDRGT
                     EDVPLIGGGGPSRRGELMELYNAVVLAKPIRHTGPWGMATLEVCLALMQSAQERKEIF
                     LKHQVPAPEDD"
     gene            complement(2073..>2754)
                     /locus_tag="VFY96_12785"
     CDS             complement(2073..>2754)
                     /locus_tag="VFY96_12785"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX5607390.1"
                     /translation="LHIGACVTHHALETTSLVREHAPALAYAESQVANVRVRNQGTLG
                     GNLAFNDPHSDPGTVLLIHDTSIKIGNRTGERRMALRDFFVDGYTTAMDPDDLLIDIE
                     IPRLPTRLKSVYRRLHRYQRPTLGVAVGVQMSDGHMSESRLAVGCIGPKPERLTELEN
                     KLNGVQPADVRRIVSEEKTYLRDLLRPVDDLLGSADYKLYMACTMLSDALESLTRSAR
                     YSGQAGDS"
BASE COUNT          604 a          774 c          781 g          595 t
ORIGIN      
        1 ttcaggaacg cctttacggc aaattccgaa taggtgcccg cgccggtgat gccgatcggc
       61 tttcctctga gctcttcgac cgtgcgcgtc tgtttgttgc cgagcaatat gtaattggtc
      121 gtactcgata tgcacccgat gatcgagaga tccagaccgc gcgccttgcc gcgaaaaatc
      181 gcctgcgcac cgccaatgct ggcgtagtcg acttcacccg aaaccagcgc cgcgatggcc
      241 gccgggccgc cgcgaataag aatcggctcg atttgcaaac cttgctgcct gaaatacccg
      301 cgcgccagag ccgctcggaa cggaatctcg ctattccagg cgaggctgac gaataccatt
      361 cggatatttc gtttctcctg cgcgtcaagc tgagccgcaa gcaacgtaac cgagagcaat
      421 gcaacgatcg acagtctatg atgtattcgc atcaaccgat gactccaatt ttcattgagt
      481 gggcctttgc ttctgccctc gtaatctaac aaatcgaccc gttgcaggaa agcaaccgag
      541 gtgagttgca gtcgctggtt cagttgcgat tttcgtcgcc gtgctcctca gcagaatgac
      601 aggacgagtg ttgacctatg ctcttgtcat cagcattagc gaaggatcta ttttgacatc
      661 atcattcgaa cctactatcg cgttgcttga catgtgcgtc actgaatctg gtaattaact
      721 taactaacct cacgaacacg gcggacccat ggctgaatca aattcaaacc gaaagttaaa
      781 gctcggcatt attggacttg gtgtcggcgc atcggaaata ttgccggcga tggagcagat
      841 cccggaattt cgactggtgg cggcggcgga tatcaatgca cgggtgctgg acatatttcg
      901 gcagcgctat ggcgcgaaaa cctatgacag catcgaaaaa ctttgtgctg atccggaggt
      961 ggaagcggtt tgggtctcta cgccgaatcg tttccacgcg ccacacacaa ttattgccgc
     1021 caatgcgggc aagcacgtcg tcgtcgagaa accgatggcg atttcgctgc aggaagcgga
     1081 gcagatgatc gaggcctcgg ttaaaaataa gatcaaattg ctctgcggcc acacgcaaag
     1141 cttcggtccg catatccgaa cgatgcggaa gattatccgc tcgggcgagt tgggcaagct
     1201 ttgcgcgctt catgtctggg cctacacgga ctggatgctt cggccaagaa ccgccgaaga
     1261 attggacatc aagcaaggtg gcggcgttcc gttccgacaa ggaccgcacc agatcgatac
     1321 tttgcgtttg ctcggcggcg gtctcgtacg gagcgtgcgc ggcgccactg gacaatggtt
     1381 caaaggccgt ccgattccag gctattattc aggcttttta gaattcgagg acggcacgcc
     1441 ggcgaccctc atgcacaatg gttatggcta ctttctcgcc tcggagctcg tcccttgggg
     1501 tggccagaat agccgatacg gcgaagccga gcgcgcggga gttcgcgcag ctctgctgaa
     1561 tggaacacgc gatgagaacg ccgacaaaga cgccatgcgc atcggcggcg ccaacgagag
     1621 agaaattcgc gaccggagca aggcaaagcc gtggctcccc aatgatctcg gaattctgat
     1681 cgcgagctgc gagaaaggcg acattcgcca atctcagcac ggtcttttcg tccacagcga
     1741 ccgaggtacc gaggacgtac cgctgatcgg tggtggcggc ccgtcgcgcc gcggtgagct
     1801 gatggagctg tacaatgccg tggtcctggc taagccgatt cggcataccg gaccctgggg
     1861 tatggcaact ttggaggttt gcctggcgct gatgcaatcg gcgcaggagc ggaaagaaat
     1921 ttttctaaag catcaagttc ccgcccccga ggacgattag caaacgaacc cagtgctctc
     1981 acctgaagtt cgttgcgcaa ccaatccttt cggcgtcata ccggcgaaag ccggtctcca
     2041 gagattctaa acttcctgga ttccgggtcg cgctagctgt cgccagcttg cccggaataa
     2101 cgagcggatc gtgtgaggct ctccagcgca tcgctcagca tcgtgcacgc catgtatagt
     2161 ttgtaatccg cgctgcccag aagatcgtcc accggccgga gcagatcgcg cagatatgtt
     2221 ttttcttcac tgacgattcg cctgacatcg gctggctgaa cgccgttgag tttgttctcg
     2281 agctccgtca agcgctcggg cttcggaccg atgcacccta ccgcgagcct gctttccgac
     2341 atgtggccat cactcatctg tacgccaacg gccactccga gggtaggacg ttgatagcga
     2401 tgcaaacgcc gatagacgct tttcaggcgg gtgggtaagc gcggaatttc aatgtcgatc
     2461 agcaggtcgt cgggatccat ggctgtcgta tagccgtcca cgaaaaaatc tcgcagtgcc
     2521 atccgcctct cgcccgtgcg attgccgatt ttgatcgagg tatcgtgaat cagcagtacg
     2581 gtcccagggt cggaatgcgg gtcgttgaac gcgagattac cgcccaaggt tccttggttt
     2641 cgcacacgaa cattggcgac ctgcgattcc gcgtatgcta atgccggcgc atgctcgcgc
     2701 accaatgagg tcgtctcgag cgcgtgatgc gtcacgcaag caccgatgtg caag
//
LOCUS       DASULZ010000621         3123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_781045, whole genome shotgun sequence.
ACCESSION   DASULZ010000621 DASULZ010000000
VERSION     DASULZ010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781045"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..906
                     /locus_tag="VFY96_12790"
     CDS             157..906
                     /locus_tag="VFY96_12790"
                     /inference="COORDINATES: protein motif:HMM:NF028512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX5607391.1"
                     /translation="MQSRSRNSFGLRWKIAGIFSALTLILSAVLIGTVYRLTEGTLRA
                     QLDKRALAVARNLSDAAAGHVLSNDLLALNTLLRKYTFLDGLAYAFIRDSRGAITAHS
                     LETFPPELREGLSAVRERVPWRRELSLQGRPVYETNVPVLDGQLGVVHVGFWRDAVEK
                     EIRRAILPLIAALGIIPLVGVALSFVLAHWIAAPIIGLTKVTKQILEGDLDASGEYPN
                     SRDEIGDLARSLERMRASLRAAMSRLQRELE"
     gene            948..2246
                     /locus_tag="VFY96_12795"
     CDS             948..2246
                     /locus_tag="VFY96_12795"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607392.1"
                     /translation="MRTAALVFGLLAFFIGIAGAAERGANSPPEKITIAYSSVSGHMA
                     PLWITHDGGFFRKHGLDVQLVFIESGSTAVKSLMSKNVAFAQMAGAGVVQSRLQGADI
                     IMIAGFLNTMDYQLMVDKNISRPDQLRGKTLAVSRFGSSSDFATRYALDKYGLKPGED
                     VTILEIGSQPARFAALEAGKIQGAMVAVPLTLKAKALGFHALADLQMLGLEYQHTGLA
                     TTQALIKSRPDLVRNVMQAYVEGIHYYKTRPAESLAILEKYLKIPDKNVLTEVYNAIG
                     LKLTPEKPYPTLRGIEVILREIAPRNSKTRIVRPEEIIDLTFVKELDTSGFIDGLYKA
                     QPVVAGRSEPRPLPESVVVKEKPSPAKRVVSNAPDGRVLPQRYTVAAGDTLGHLALRF
                     YWLSTKWPQIQQANADTLANPHYLYIGQELLIPPDARTGK"
     gene            2564..2788
                     /locus_tag="VFY96_12800"
     CDS             2564..2788
                     /locus_tag="VFY96_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607393.1"
                     /translation="MLAQIVPLCQVHDARACSYMFNCQIDALNQPPLPLIPLFDLAAD
                     FPEGLFHFHGEVMKVSCLVMQKEESDYLMV"
BASE COUNT          752 a          812 c          809 g          750 t
ORIGIN      
        1 ccacactcat ttctttctga cggactgtca gttggtacaa cagaacaacc agctcaatgg
       61 ccaaccaaca ccgaggaggc aggttccgac cggtcgcagg cgcaaaccgc accgaatgcg
      121 cagttagcag gggcgtttag atctcccaga gccgtgatgc agagtcgatc tagaaatagc
      181 ttcgggctcc ggtggaaaat tgccgggatt ttttctgcgc tgacgctaat cttgtccgct
      241 gtccttatcg gaaccgtata ccgcctcacc gaaggcacgc tacgtgcgca gctcgataaa
      301 cgagccttgg ccgtagccag gaatctgagc gatgcggcgg ccggtcacgt gctgagcaat
      361 gatcttctcg cgcttaacac tctcttacga aagtatactt ttctcgacgg cctagcgtac
      421 gcatttattc gggatagtcg aggcgctatc actgcgcaca gtttggaaac atttccgcct
      481 gaactgcgcg aagggctatc tgcggtcaga gagcgtgtgc cttggcggcg cgaattatca
      541 ttgcaaggaa gaccagtata cgagacaaac gttcctgtcc ttgatggcca gcttggtgtc
      601 gtccatgtcg gtttttggcg agatgccgtt gagaaagaaa tccgtcgggc tattctccct
      661 ctgatcgcag ccctggggat catacctcta gtcggtgtgg cgctctcgtt tgttttagcg
      721 cactggattg ctgcgccaat cattgggctg acaaaagtta cgaaacaaat tcttgaaggc
      781 gatctggacg catctggaga atacccaaac tctcgcgatg aaatcggcga cctggctcgc
      841 tctctggagc ggatgcgcgc gagccttaga gccgctatgt cacgtctcca gcgcgagcta
      901 gagtaaaaaa ctcgagacca atgtatatca aagaagacca ccaacccatg aggacggcgg
      961 ccctggtgtt tgggctgctg gcgtttttca ttggtatcgc cggcgcggcg gagaggggag
     1021 caaattcacc accggaaaag atcacgattg cctacagctc tgtttcgggc cacatggctc
     1081 cgttgtggat cacgcatgat ggcggtttct tccgtaaaca cggactggac gtgcaactcg
     1141 tttttatcga gagtggaagc actgcagtca aaagcttgat gtcgaaaaat gtggcattcg
     1201 cgcagatggc gggcgccggg gtagtccaaa gtcgtttaca gggagccgat atcattatga
     1261 tcgcgggatt tctgaacact atggattatc agctcatggt tgacaaaaac ataagccggc
     1321 cggatcagct tcgtgggaaa acattggccg tcagtcgctt tgggtcttcg tctgacttcg
     1381 ccacgcgcta cgctctggac aaatacgggc tcaaacccgg tgaagatgtg acgattctgg
     1441 agatcggaag ccagccggcc cgcttcgcag cgcttgaggc cggcaaaatt cagggagcga
     1501 tggttgcggt tccgttaaca ctgaaggcca aggcgcttgg ctttcatgcc ctcgccgatc
     1561 ttcaaatgct cggattggaa tatcagcaca ccggcttggc tacgactcag gcattgatca
     1621 aatcccgacc tgacctggtt cgcaacgtca tgcaagctta tgtcgaaggg attcactact
     1681 ataagacgcg gcccgctgag tcgttggcca ttttagaaaa gtatctaaaa atcccggata
     1741 aaaatgtgct gacggaagtg tataacgcta ttggactgaa gctcactccg gagaagccat
     1801 atcccacgct ccgcgggatt gaggtcattt tacgtgagat agcgccgaga aactcgaaga
     1861 caagaatagt acggcccgag gagataatag atttaacgtt tgtcaaagaa ttggacacct
     1921 ccgggttcat cgatggtttg tataaggctc agccggtcgt cgcggggcgt agcgagccac
     1981 ggcctttacc cgaatccgta gtagttaaag aaaagccctc ccccgccaag cgtgtcgtct
     2041 cgaacgctcc tgacggccgt gtcttgccgc aaagatatac tgtggcagcc ggtgatacac
     2101 tcggacacct ggcattgcgt ttctactggt tgtcgacaaa atggcctcaa attcaacagg
     2161 cgaatgcgga tacgttagca aacccgcatt atctttacat aggacaggag cttctgattc
     2221 ctccggatgc tagaacggga aaatgacggc tgcaaacgag cggttgaaga agtcaaatcg
     2281 acgcttaaaa gtctcgacga cgaaaatgtt ctcactaaca tcgatcagct gattgagccg
     2341 ttgtttttgc gacccgccga taaattgctt gccttgaaag cgggcgacgc agagtgcgtg
     2401 cttaaaacgc gcgagaatgg attgaatcaa gatttcgaga ggaggactga tcctcaggat
     2461 tgagctattt ttctttccaa cttacctctc agctgcaata tttctctcaa acattcgact
     2521 gcgcgccaaa aacaccgcat tatcgattga atcggcgcac ttcatgctcg ctcaaattgt
     2581 gccgctttgc caggtccacg acgctcgcgc ctgtagttat atgttcaatt gccaaatcga
     2641 tgcactgaac caaccgcctc tgcctcttat ccctttgttc gatttggcgg cggattttcc
     2701 cgagggcctt ttccactttc atggcgaggt catgaaggtc tcttgtttag taatgcaaaa
     2761 agaggaaagc gattatctaa tggtgtgatg ccaaaaaaac tttaccgctc attgacgtcg
     2821 gttctaagtc cccaaaatgg gttggtatcg atctaccccg aaagcgcgca caccaaaggc
     2881 taggtttctt tcgccagatc tctcaccgca ttttttcttc gacaattcgg ttttctctcc
     2941 ggtccttgtg ccgccattgc tcagcccgtc acgtcacatt agtcacaaca cgggcgtaga
     3001 acatgtactt caggtgactc gtctatcgtt cgctagacaa taattccgat taccgctggc
     3061 ccatcttttg ccccgttcgc accgtgaagc tccgaaaaga acacagcatg gaggaaaact
     3121 atc
//
LOCUS       DASULZ010000622         2813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_680191, whole genome shotgun sequence.
ACCESSION   DASULZ010000622 DASULZ010000000
VERSION     DASULZ010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680191"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..613)
                     /gene="scpB"
                     /locus_tag="VFY96_12805"
     CDS             complement(38..613)
                     /gene="scpB"
                     /locus_tag="VFY96_12805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX5607394.1"
                     /translation="MEREEIKSILESLLFVADGPQSAQRLAESIDGIDRKLIQEILAE
                     FQTELETQNRGVRLVEVAGGYQLRTPKNNADWVKKFLGGRPARMGKATLETLAIVAYR
                     QPITKAEIEAIRGVDVDGVITTLLERSLIRAIGRKDVPGRPFLYATTPEFLQLFNLKD
                     LSGLPPLKEMEEMSLPEFSGESIVIEQAEES"
     gene            complement(603..1364)
                     /locus_tag="VFY96_12810"
     CDS             complement(603..1364)
                     /locus_tag="VFY96_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX5607395.1"
                     /translation="MSTHVQLEIFEGPLDLLLHLIKKNEVSITDIPIAAITEQYLAAL
                     ELMQSLNLDVAGEFLVMAATLIHIKSRMLLPAGADEPDEDEGIDPRDELVRRLLEYER
                     YKEAASVLEQREILTRDVFIRGGGSTEEVGPRAFREISIFELLGALKRVIERLPKDVF
                     HEVTLEKITVREKMTLLLDRLHERRSLVFETLFDQAKSRMEVVVTFLAMLELVKVRAI
                     RIVQEEPGGPILIEAAAGMDEAAEQVALDEEQNGA"
     gene            complement(1361..2338)
                     /gene="trpS"
                     /locus_tag="VFY96_12815"
     CDS             complement(1361..2338)
                     /gene="trpS"
                     /locus_tag="VFY96_12815"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX5607396.1"
                     /translation="MEIIVSGMRPTGRLHLGHFHGALQNWLRLQQEYQCYFFVADWHA
                     LTTDYATPQGIQQNTLEMVMDWMSIGLDPAKAVIFRQSQVKEHAELHLIYSMITPVPW
                     LERNPTYKEQIKELTGKDLSTYGFLGYPVLQAADITIYKANKVPVGVDQAPHVELTRE
                     IVRRFNQLYQPIFPEPEVLLTEAQKVPGLDGRKMSKSYGNAVFLSDTPQEIDQKLSRM
                     MTDPARVRRSDPGEPEKCPAFQLHKIYCTSEEIDHVSAGCRTAGIGCLECKKIMIKHV
                     IEDLAPFREKRAHYQDKPEEVAAILTSGKDVAAKAAAATMTEVRGTLGL"
     gene            complement(2349..>2813)
                     /locus_tag="VFY96_12820"
     CDS             complement(2349..>2813)
                     /locus_tag="VFY96_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5607397.1"
                     /translation="PFVFGYAKPVPVNFSNLRNPKRDMIWVAAAGPAMNLLLALFCSI
                     AWRLLAPGIGDHGEQSGASRLSDYTAALVLMAQSGILINIVLAVFNMLPLPPLDGGRV
                     MVGLLPPSLSMALAKIEPYGFIILIVLLMTHTLDRIIDPPTEFLLRLYLGAR"
BASE COUNT          616 a          830 c          722 g          645 t
ORIGIN      
        1 caatgatttt ttgcaaacgt tccatagcag agcgccatca gctctcctcg gcttgctcga
       61 tgacgatgct ctcaccggaa aattccggca gcgacatctc ttccatttct ttcagcggcg
      121 gtagcccgga caggtccttg agattgaaca gctgcaaaaa ctccggtgtg gtggcgtaga
      181 gaaacggccg accgggaaca tctttgcgtc cgatcgcacg aatcaaactg cgctcgagca
      241 atgtcgtgat cacgccatcg acatcgacgc cccgaatcgc ttcgatctcg gcttttgtga
      301 tcggctggcg ataggcgaca attgcgaggg tctccagggt cgctttgccc atccgagccg
      361 gccgtccgcc aagaaatttc ttaacccagt cagcattgtt tttgggcgtg cgtaactgat
      421 agccaccggc gacttccacc aagcgcacgc cgcgattttg cgtctctagc tcagtttgga
      481 attcggcgag aatttcctgg atcagtttac ggtcaattcc gtcaatgctt tcagccagtc
      541 gctgcgctga ctgcggtcca tcggcgacaa acaacagact ctcgagaatt gatttaatct
      601 cttcacgctc cattttgttc ctcgtccaag gcgacttgtt ccgcggcttc gtccattccc
      661 gccgcggctt caatgagaat cggaccgccc ggctcttctt gaacgatccg gatcgcccgc
      721 accttgacca gctccaacat cgcgagaaac gtcaccacga cttccattcg acttttcgcc
      781 tgatcgaaga gcgtctcgaa aaccaggctg cggcgctcat gcagacgatc caggagcaag
      841 gtcattttct ctctaacggt aatcttctcg agggtgactt catggaaaac atctttgggc
      901 aaccgttcga tcacccgttt taaggcgccg agcagctcga aaatggaaat ctcacgaaac
      961 gcgcgcgggc cgacttcctc cgtggaaccg ccgccccgga taaacacgtc gcgtgtcagg
     1021 atctcgcgtt gttcgagcac gctggcggcc tccttgtagc gttcatattc gagaagccgc
     1081 cggaccagct cgtcgcgcgg gtcgatcccc tcgtcttcat cgggttcatc ggcacccgcg
     1141 ggaagcagca tacgggattt gatgtgaatc aaggtcgcgg ccatgacgag aaactcaccg
     1201 gcaacatcaa gattcaggct ttgcatcagc tcgagagcgg ccagatattg ttcggtgatc
     1261 gccgcaatcg ggatgtccgt aatgctgacc tcgttctttt tgatcagatg cagcaaaagg
     1321 tcgagcggac cctcaaaaat ttccagctgg acgtgagtgc tcacaacccc aaagttcctc
     1381 tcacttccgt catggtcgca gccgcagctt ttgccgcgac atcttttccc gacgtcagaa
     1441 tcgctgccac ctcttccggc ttgtcttgat aatgtgctcg cttctcgcgg aacggcgcga
     1501 gatcctcaat gacgtgcttg atcataatct tcttacattc caagcagccg atccccgctg
     1561 tacggcagcc cgccgaaacg tggtcgattt cctccgatgt acaataaatt ttgtgcagct
     1621 gaaacgctgg gcacttctcg ggctcgcccg gatcgctccg tcgcacccgc gccggatcag
     1681 tcatcattcg gctgagcttc tgatcgatct cctgcggcgt atcggaaaga aacaccgcgt
     1741 tgccgtagct tttgctcatt ttgcgcccgt cgagacccgg aaccttctga gcttcggtca
     1801 gcagcacttc cggctctgga aaaattggct gataaagctg attgaatcgc cgtacgattt
     1861 cgcgggtgag ctcgacgtgt ggcgcctggt cgaccccaac cggaactttg ttcgctttgt
     1921 agatcgtaat atcggccgct tgtaaaactg gatagccgag aaaaccgtag gtcgacagat
     1981 ctttgccggt cagctccttg atctgctctt tataagtcgg gttgcgctcg agccacggca
     2041 ccggcgtgat catcgaataa atcaagtgaa gctcggcatg ctctttaact tgcgattggc
     2101 ggaagatcac cgccttggcg ggatcgagtc cgatactcat ccagtccatc accatctcca
     2161 gggtgttttg ctgaatcccc tgtggtgtcg cataatcggt cgtcagagcg tgccagtccg
     2221 cgacgaaaaa atagcattga tattcttgct gaaggcgcaa ccagttctgc aatgcgccgt
     2281 ggaaatgtcc cagatgtaac cgcccggtgg gccgcatccc gctcacaata atttccataa
     2341 atgtccgact agcgcgctcc caggtaaagc ctcagcaaga attccgtcgg cggatcgatg
     2401 attcggtcca acgtgtgggt catcagcaaa acgatcaata tgataaaacc gtagggttcg
     2461 attttcgcca aagccatcga tagacttggg ggcaacaggc cgaccatcac tcgtccgcca
     2521 tccagcggcg gcagtggcag catattgaaa acggcaagaa cgatgttgat caaaattccg
     2581 ctctgtgcca tcagcacgag tgctgccgta tagtcggaaa gcctcgaagc gccagactgt
     2641 tcgccatgat cgccgatccc tggcgccagc aacctccacg caattgagca gaacaacgcc
     2701 agcagcaaat tcattgcggg accggcggcc gccacccaga tcatgtcccg tttagggtta
     2761 cgcagattgg aaaaattcac gggcaccggc ttcgcgtagc cgaagacaaa cgg
//
LOCUS       DASULZ010000623         2956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_680275, whole genome shotgun sequence.
ACCESSION   DASULZ010000623 DASULZ010000000
VERSION     DASULZ010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680275"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1028)
                     /locus_tag="VFY96_12825"
     CDS             complement(159..1028)
                     /locus_tag="VFY96_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607398.1"
                     /translation="MVAAETRTLMDKCRPSRKQVCHRFGGSDWVFAMAATGLIALWPA
                     KAQAITISHNDALKVGKKVWQNECGGTIAGLTSWNQGEDFASLGIGHFIWYPKGRRGP
                     FEESFPELVKFISKRGAKLPTFLLGAGGQACPWNSRSEFLQAQNTAEMNQLRRFLADT
                     IDFQAEFLVARLEAALPKMLAEAAPADRTNVQQQFERLTKTPQGCYALIDYVNFKGEG
                     VLHTERYQGQGWGLLQVLETMHGNSDSSASDEFARAAKIVLTRRVQNAPPDRHESRWL
                     TGWLRRVNSYSGG"
     gene            1062..1820
                     /locus_tag="VFY96_12830"
     CDS             1062..1820
                     /locus_tag="VFY96_12830"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX5607399.1"
                     /translation="MKCFIVIVSASLLAFPLFGQEKSPQLKDQKDKVSYSIGMQIGFN
                     LGRQKVDINPDILAAGIKDAIAGKPQLTTDQVKDIMAQFEKDMETKQKQLGEKNKTDG
                     TKFLEDNKKKPGVKTTASGLQYKVEKEGTGATPKATDMVTVNYRGTLIDGTEFDSSYK
                     RGQPATFPVNGVIKGWTEALQLMKQGSKYQLYIPSTLAYGERAMGPDIGPNSTLIFEV
                     ELQDVKPPPTPAPQAATQGSPKAPAPPSPAPTKK"
     gene            complement(1971..2336)
                     /gene="rsfS"
                     /locus_tag="VFY96_12835"
     CDS             complement(1971..2336)
                     /gene="rsfS"
                     /locus_tag="VFY96_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012463699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX5607400.1"
                     /translation="MAITPENLAKTCAELASNKKAEDIVVLDLRGISSFTDFFVICSG
                     TSEPQLKAIAGEIESRLREEYSLRPVAVDGFPASQWIVLDYLQVVVHIFHRDKRAFYS
                     LEGLWGDARAMRWESVSSP"
     gene            complement(2327..2896)
                     /gene="nadD"
                     /locus_tag="VFY96_12840"
     CDS             complement(2327..2896)
                     /gene="nadD"
                     /locus_tag="VFY96_12840"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX5607401.1"
                     /translation="MKKIAIYGGTFDPIHHAHLILAREAIETLDLDKVILVPAAISPL
                     KKAAPVANGEVRLTMLRAAIKGDPKFEVNECELLRPPPSYTIDTVEEICRRECDASIY
                     CLIGEDNVGQLPQWHRFGELEKMVRFVVLDRSGKQPSHSYPLIHRRIDISATEIRRRV
                     AQNESIRYLVPDSVDEIIQREKLYREQLQ"
BASE COUNT          719 a          803 c          772 g          662 t
ORIGIN      
        1 gacggttaat gtaagcggac gctttgcgca tctgctttgg ttgtggcgtc ggccttggtg
       61 atctcggtcc aaaaaggcga aactgagcgc tgatcgacga cgcgagggcc gtcaacaaga
      121 gcgcgtaaag aagggtgcga agttttcccg gcataatatc agccgccact gtagctattt
      181 acacgccgaa gccaacccgt taaccatcgc gattcgtggc ggtcgggagg agcattctgg
      241 acacggcggg ttaaaacaat tttggccgcc cgcgcaaact catcagacgc gctcgaatcc
      301 gaattgccgt gcatcgtttc aagtacctgc agcaaacccc atccctgacc ctggtagcgc
      361 tccgtgtgca gcacgccttc ccctttgaag ttaacgtaat cgatgagggc gtagcatccc
      421 tgtggcgttt tcgtgaggcg ttcaaattgt tgctgcacgt tagttcggtc ggcaggcgcc
      481 gcttcggcca gcatcttagg cagagcagct tccaatctgg caacaagaaa ttctgcctgg
      541 aagtcaatag tgtcggccag aaatcggcgc aattgattca tttcagctgt attttgcgct
      601 tgcagaaact cggaacgcga gttccaggga cacgcctgtc ccccagcgcc cagaaggaat
      661 gttggcaact tcgcgccgcg tttcgatatg aattttacca gctctgggaa actttcttcg
      721 aacgggccgc gccgtccctt tggataccag atgaagtggc cgatgcctag cgaggcgaaa
      781 tcttcgccct ggttccacga agtgagtccg gcaattgttc caccacactc attttgccac
      841 acttttttcc cgactttcaa agcgtcattg tgcgagatcg tgatggcctg cgccttggcc
      901 ggccagagtg cgatcagtcc ggtggctgcc attgcaaata cccaatcact gccaccaaac
      961 cgatggcaaa cctgttttct tgacggacgg catttatcca ttagcgtgcg ggtttctgcg
     1021 gcgaccaacc ttaaactagc gcggcgcgga aacacgatct tatgaaatgt tttattgtta
     1081 tcgtcagcgc atccttactt gcattcccat tgttcgggca agagaaatca ccacagctca
     1141 aagaccaaaa ggataaggtc agctacagca tcggcatgca aatcggattt aatctcggcc
     1201 ggcagaaagt ggacattaac ccggatatcc ttgccgccgg cattaaagac gccatcgctg
     1261 gcaaaccgca actgacgacc gaccaggtta aggatatcat ggcccagttc gaaaaggaca
     1321 tggaaacgaa acagaaacaa ctgggcgaaa aaaataaaac tgatgggacg aagtttcttg
     1381 aagacaacaa gaagaaaccc ggtgtgaaaa cgactgcgag cggtttgcaa tacaaagttg
     1441 agaaggaagg aaccggagcg acgcccaaag ccaccgacat ggtgacggtg aattatcgag
     1501 gcactttgat cgatgggacg gagtttgata gttcgtacaa gcgcgggcaa ccggcgactt
     1561 ttccggtcaa cggcgtgatc aaaggctgga cggaagcgct tcaactgatg aagcagggat
     1621 caaaatatca attgtacatt ccctcgaccc tcgcctatgg cgagcgtgcc atgggcccgg
     1681 acatcggtcc gaactcaacg ctgatttttg aggtcgaatt gcaggatgtg aaaccgcctc
     1741 caacgcctgc gccacaggcg gccacacagg gttctccgaa ggctcctgct ccgccgtcac
     1801 cagcgccaac caagaagtag cgcgaaggag taatttatca ctctgccgcg ccattcttat
     1861 gcgcgcggcg tggtgaatgc cgtttggcgt gtttccagag gtcggatcgc gccgttcgaa
     1921 gtgggtaggt ttagcacgct ttgcggctcc agtgcgccgc aacgcctttg ctaggggctc
     1981 gacaccgatt cccaacgcat ggccctcgca tcgccccaca agccttccaa gctataaaag
     2041 gcgcgtttat cgcgatgaaa aatgtgaacg accacttgca gataatcgag aactatccac
     2101 tggcttgcgg gaaatccgtc caccgccacc ggtcgcagcg aatattcctc gcgtaagcgt
     2161 gactcaattt cgccagcaat cgccttgagt tggggctcag acgtgcccga gcagatcaca
     2221 aagaaatccg taaaactcga gatgccgcgc aaatccaaca cgacaatgtc ttccgccttt
     2281 ttattggagg cgagttccgc gcaggtcttt gctagatttt ccggcgttat tgcaattgct
     2341 cccgataaag tttttcgcgc tgaataatct cgtccactga atcgggtacg agataacgaa
     2401 tcgattcgtt ttgcgcaact cttcgtcgta tctcagtagc ggaaatatcg atgcggcgat
     2461 gaatcaacgg ataagaatgt gagggttgtt tgccagagcg atcgagaacc acgaaccgaa
     2521 ccattttctc cagttcccca aaacgatgcc actggggcaa ttgtccaacg ttgtcttcac
     2581 cgatcaggca gtagatggat gcgtcgcatt cgcgtcggca aatttcttca accgtatcga
     2641 ttgtgtacga aggcggcggt cgcagcagct cgcattcatt tacttcaaac ttcggatcgc
     2701 ctttgatcgc cgctcgcaac attgttaatc gtacttcgcc gttcgcgacc ggggcggcct
     2761 tcttgagagg ggaaatcgcc gccggcacca ggatgacttt atcgaggtcg agcgtttcaa
     2821 tcgcttcccg ggcaagaatc aaatgcgcgt gatggatcgg gtcgaaggtg cccccataaa
     2881 tcgcgatctt cttcaatccc ccaacgctta aacgctcaac gcttaacgtt caactaaaag
     2941 cgttctatct gaagac
//
LOCUS       DASULZ010000624         5255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_781253, whole genome shotgun sequence.
ACCESSION   DASULZ010000624 DASULZ010000000
VERSION     DASULZ010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781253"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1035)
                     /locus_tag="VFY96_12845"
     CDS             complement(175..1035)
                     /locus_tag="VFY96_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607402.1"
                     /translation="MQGPESSKQPAKEAIARVFPESLIAAPEKRLASQRVEYNTSIAT
                     RRPEARQNSAWQYRINRRDEIVGFEFSNHGGNRILPARRDASKNQFYTRDFQFHFDER
                     SRQDIHLMISDWVASRDRTFRLSEVMNSVMLFFPRMWLPAIVNIADRNVVTLPTGEEV
                     QFDAQTHEIRSGVLSEAPVDVNPQSTPRKFPEVSYHGNGIVVRADAKGKDPRIATNAT
                     IVSRPQTQNCGDSIPCNQCQIPSNELWHQSGAVRFKFADDEAFDRFLITRCGFGLPRI
                     GNDFAVVTKQ"
     gene            complement(1332..2015)
                     /locus_tag="VFY96_12850"
     CDS             complement(1332..2015)
                     /locus_tag="VFY96_12850"
                     /inference="COORDINATES: protein motif:HMM:NF021141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2459 domain-containing protein"
                     /protein_id="HEX5607403.1"
                     /translation="MLLRVFAQKMIPALSCFLFFLGFTDPAPAHWACGAIESGCKTVF
                     IVHGGWHAAIVLHAADISPAIVPEIADFPQVRFIEFSWGDKDYFPDPGAGPFTAMKAA
                     FSSSGSVLHLVGVTEEVARFYSGAEIIKLQFAATAFGELLSYLSETFARPQPGVRARS
                     RPGLYPDSRFYPGTRRFALWNTCNTWVADALAAGGFPISPSFVVTAGQLSEQISKVKS
                     HAIVFEQRS"
     gene            complement(2059..3552)
                     /locus_tag="VFY96_12855"
     CDS             complement(2059..3552)
                     /locus_tag="VFY96_12855"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607404.1"
                     /translation="MTTFPSANAIRFADTTLRDGHLSLWASNMRTDMMLPIARRLDDA
                     GFEAIEVMSSAFYKKCVRDLKEDPWERIRLLKQHIRRTPLRSIRSRSMLAFQMTHPAI
                     ADLWLERLAANGIDELRTSDPSNTPRYWKEAVDAAKRAGLKTILNIIYSISPKHTDEY
                     FVQRAREAVKLEVARICFKDPGGLLTPESTRRLVPLILRETKAIPLEFHTHCNTGLGS
                     LCCLEAIKLGVTSINTAIPPLADGSSNPSIFNVAMNARALGFTTRIDEEILRPVRDHF
                     VKVAEREHLPLGKPVHYDALHPIHQVPGGMISNFRFQLASLGKLDKLPEVLEEVSRVR
                     AEFGYPIMVTPYSQFFGVQAAINVIVGQRYKEVTDEVLLYTLGFWGDEEARYIDGNVK
                     DRLLSIPRAKELARSDPPTLSIQEFRRKFGGETVSDDDKLLYYFAGAEAVNAMRAGHG
                     HSSVRPNPNASLITLIERIGQRKQAVHVYIKRKGLSLRIQRRATANA"
     gene            complement(3585..3914)
                     /locus_tag="VFY96_12860"
     CDS             complement(3585..3914)
                     /locus_tag="VFY96_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607405.1"
                     /translation="MKFFCLHSGTDGQSHFEPLDTSERSEFLNATRPATGLLIRTAFA
                     PHIVNFHRAPRRRWVITLAGSVDIGLGDGTSMTFHPGDIFLAEDTTGQGHTATPHDWI
                     RAYVNLD"
     gene            complement(4273..4590)
                     /locus_tag="VFY96_12865"
     CDS             complement(4273..4590)
                     /locus_tag="VFY96_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607406.1"
                     /translation="MTRALGRISLVLFLTVAVGYQSWSVIVPPDSTDFLFTATPAPKL
                     IPSLFDIEDEHRGAHASLSILPPPLILFGASVLLAQTRGHFFSELRFNYSTLQTMSVV
                     LLI"
     gene            complement(4625..>5255)
                     /locus_tag="VFY96_12870"
     CDS             complement(4625..>5255)
                     /locus_tag="VFY96_12870"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX5607407.1"
                     /translation="DFNKISEVFDKAIDELKNAGAEIIDPVVIPNLKNLLAKRAGSFA
                     DEEQSFKNFMNRSKNPPYRSKQDAVRSPEFDKVVKHSRERWLRAASEDANYEYLKARE
                     ELMTQFLKVMAEHKLDAIVHKAVEHQPTRIADGVNPPYVDQKGAPHLNTFLVYVPSIV
                     VPAGFTRDHLPAGVTFLGRPYDDGNMIKFAYAYEQATRHRQPPASATPM"
BASE COUNT         1218 a         1372 c         1503 g         1162 t
ORIGIN      
        1 gcgaacgatc aaatcattct ttacgtgata gggttgcttc gctaattgca accgacaact
       61 ctactgagaa aggacgggac ggttaacgag gatcttgtgc tggtgcgtaa ttccacatga
      121 cgaagcggcg aaggaggaaa aaaagaattg ccctgaaagc gcggttgcgc gaatttactg
      181 tttcgttaca accgcgaagt cattaccgat ccggggcagc ccgaagccgc agcgcgttat
      241 caaaaagcga tcaaacgctt cgtcgtcagc aaatttaaag cgtacagcgc cgctctgatg
      301 ccatagctcg tttgagggaa tttgacactg attacagggg atcgaatctc cgcagttttg
      361 cgtctggggt cggctcacga ttgtcgcgtt cgtcgcgatt ctcggatctt tgcctttggc
      421 gtcagcgcgt accacgatac cgtttccgtg ataactgacc tcgggaaact ttcgaggcgt
      481 gctttgagga ttgacatcga ctggtgcttc ggacagcacg cctgaccgaa tctcgtgagt
      541 ttgagcgtca aattgcactt cttctccggt cggcaaagtg accacgttgc ggtccgcgat
      601 gttcacaatt gcaggtaacc acatgcgtgg aaagaaaagc atgacactgt tcataacttc
      661 gctgagccga aatgtacggt cacgcgatgc tacccagtcc gatatcatca gatgaatgtc
      721 ctggcgcgag cgctcatcaa agtggaattg aaaatccctg gtatagaatt gattctttga
      781 tgcatcgcgg cgcgccggca ggatgcggtt gccgccgtga ttggaaaatt caaatccaac
      841 gatttcgtcg cgccgattga tgcgatactg ccatgcggaa ttttgccgtg cttcagggcg
      901 cctagtggca atggaagtgt tgtactcgac gcgctgagag gccaggcgtt tttccggcgc
      961 ggcaatgagt gattccggaa acaccctggc aatcgcttcc ttggctggtt gtttgctcga
     1021 ttcaggtccc tgcacagggc tgggctcgtt ggcgggaaga ttggcttgag gaggactttc
     1081 cggtgtcggg gccggctcag aaggtccgcg ctccggcgag atacgacaac cgcccaaaag
     1141 aacagcgctc cacaaacata gccaaagcac gacggtttga attctctgaa ccattttcat
     1201 ctgaaaaact agagtaacgt tcccaatccg gctgcgcaac gttcgtgccg acgtggctat
     1261 ctcctagtga gcagacacaa acgcggatca tcacgtattt ctggcagccg tacggcttct
     1321 cgtgtgatcg attatgaacg ctgctcgaac acaattgcgt gcgatttaac tttgctgatt
     1381 tgctcggaga gctggccggc ggtcacgaca aagctgggtg agattggaaa tcctccggcg
     1441 gctagagcgt cggcgaccca ggtgttgcaa gtattccata gagcgaaccg ccgtgtcccg
     1501 ggataaaatc gactgtcggg atacaaaccc gggcgcgaac gggcacgcac tccgggctgt
     1561 ggtcgcgcaa aggtttcgga gaggtaactt aggagctcgc cgaatgctgt cgcggcgaac
     1621 tgcaatttga tgatttcagc gccggaataa aatcttgcaa cttcctcggt aactccaacg
     1681 agatgcagca cgctgccgct cgacgaaaag gcggccttca tcgcggtgaa cgggccggcg
     1741 ccgggatccg gaaaataatc cttatcgccc caactgaatt cgataaaccg cacctggggg
     1801 aaatcagcaa tctccggcac gatcgcggga gaaatatcag cggcgtgcag gacgatagcg
     1861 gcatgccagc cgccgtgcac gatgaaaacg gttttgcaac cagattcaat ggcgccgcag
     1921 gcccaatggg cgggtgccgg atcggtgaac cctaaaaaga acaaaaagca ggataaggca
     1981 gggatcatct tctgagcaaa gacacggagc agcatcgctg aacaactttc gaacgcagag
     2041 agtgagcctg ttttcaactt acgcgttggc tgtcgctcgt cgttggatgc gcaaactcag
     2101 ccccttgcgt ttgatataga cgtgcaccgc ctgcttacgc tggccgattc gttcaatcaa
     2161 ggtgatcagc gaagcattgg gattggggcg gacggacgaa tgtccatggc cagctctcat
     2221 ggcgttgacc gcttccgctc cggcgaaata atacagaagc ttgtcatcat cgctgacggt
     2281 ttcgccgccg aatttccggc gaaactcctg gatcgacagc gttggtggat cagaccgggc
     2341 aagctccttt gcgcgcggaa tgcttaataa ccgatctttc acgttgccat cgatgtaacg
     2401 cgcttcttca tcgccccaaa agccgagcgt gtacagcagc acttcatcgg tgacttcttt
     2461 gtagcgttgg ccgacgatga cattgatcgc ggcctgcacg ccgaagaatt gcgagtaagg
     2521 cgtgaccatg atcggataac cgaactcggc acgaacgcgc gacacctctt cgagaacctc
     2581 gggcagcttg tcgagtttgc ccagactggc gagctgaaaa cgaaaattgg aaatcattcc
     2641 tcccggcacc tgatggatag gatgtaatgc gtcgtagtga acgggtttgc cgagcggcaa
     2701 gtgttcgcgc tcggctacct tgacgaagtg atcgcgcaca ggtcgtaaaa tctcttcgtc
     2761 gatgcgagtc gtgaacccga gggcgcgggc attcatcgcc acgttgaaga tggacggatt
     2821 cgaggagccg tcggccagcg gcggaatcgc cgtattgatc gaagtgacgc cgagcttgat
     2881 ggcttcgaga caacagaggg aacccagtcc agtgttgcag tgggtgtgaa attcgagtgg
     2941 gatcgctttg gtttcccgta gtatcaatgg gaccaagcgc cgggttgatt ccggcgtcag
     3001 caagccgccg ggatctttaa agcagatgcg cgcgacctcg agcttgacgg cttctctggc
     3061 ccgttggacg aaatattcgt cggtatgctt aggcgaaatt gaatagatga tgttcaaaat
     3121 ggttttcagc cccgcccgct ttgcggcatc gacagcttct ttccaatagc gcggcgtgtt
     3181 cgacggatcc gaagtacgca gttcgtcgat accgttggca gccagccgct ccagccaaag
     3241 gtcggcaatt gccggatggg tcatttgaaa agccagcatg gagcggctcc ggatcgagcg
     3301 cagcggtgtc cggcgaatgt gttgcttgag aagacgaatg cgctcccagg ggtcttcctt
     3361 gagatcgcgc acgcattttt tgtaaaacgc cgacgacatc acttcgatcg cctcgaatcc
     3421 cgcgtcatcc aggcgccgcg caatcggtag catcatgtcg gtgcgcatgt tcgatgccca
     3481 gagactcaga tggccatcgc gcagagtcgt gtcggcgaag cgaatcgcat tggctgaagg
     3541 aaacgtagtc atgaacggct gctgagctcc gtctggaacc tcgctcaatc gaggtttacg
     3601 taagcgcgaa tccagtcatg aggtgtcgcg gtgtgtcctt ggccggtggt gtcttccgcg
     3661 agaaagatat cgccaggatg aaaggtcatg ctcgtgccgt cgccgagacc aatgtccacg
     3721 ctaccggcaa gcgtaatgac ccaacgccgt ctcggggcac ggtgaaaatt gacgatgtgc
     3781 ggcgcgaaag cggttctaat cagtagtccg gttgccgggc gagtcgcatt taaaaactcc
     3841 gagcgttcgc tggtatcgag cggttcaaaa tgcgattgac cgtctgtacc tgaatgtaag
     3901 caaaaaaact tcatcgccaa cctcacatcg gaaccatgac cgaatcatga tgatagcgca
     3961 attggatgtc cagcgcagcg caacggaggc ggatttcgtg ttcacatcct tccaggaggg
     4021 cggcgatcga ttccaccctc ctggagaatg gttatttaag cctgcaaata agaatggagc
     4081 atgccccaga ttgaatagag cacagtcagt ccgatggtgc cccagatcac cagttttagc
     4141 caaatgggaa aatcttccat gtcgatctcc tcaaaactgc tcgacgcgtt gcctcgcgca
     4201 aacggcgcac ttcgcgaggc ggcgtcaatc aaacgctctt gaatgagtga gcctgcactt
     4261 cagaggagag actcaaatga gaagaacgac gctcattgtt tgcagcgtgc tgtaattgaa
     4321 ccgaagctct gaaaaaaaat gaccgcgagt ctgagccaac aaaaccgatg cgccaaacaa
     4381 aatgagcgga gggggaagta tcgatagcga cgcatgggcg ccacgatgct catcctcgat
     4441 atcgaacaat gacgggatca gcttaggggc gggagttgcg gtgaagagaa agtcagtgct
     4501 gtctggaggc acgataaccg accagctctg ataaccaacc gccacggtga gaaacaatac
     4561 caggctgatt cgacccagtg cacgcgtcat tcctgcctcg cttctcagca aactcttatc
     4621 ctcgttacat cggtgtcgca ctggctggtg gttgccgatg ccgcgttgct tgctcatagg
     4681 cataggcgaa tttgatcatg ttgccgtcgt catacggccg gccgagaaaa gtcacgcccg
     4741 cgggtagatg atcgcgggta aatccggcag ggacgacaat cgagggcacg tagaccagga
     4801 acgtgttgag atgcggtgcg cctttctggt caacgtaggg cgggttgacg ccatcggcga
     4861 tccgggtcgg ctggtgctcg actgccttat gaacgattgc gtccagctta tgctccgcca
     4921 tgacctttag aaactgggtc atcagttcct cacgtgcctt gagatactca taattcgcgt
     4981 cctcactcgc cgctctgagc cagcgctcgc gcgagtgctt gaccacttta tcaaactccg
     5041 gtgagcgcac ggcgtcctgt ttcgatcgat agggcggatt cttgctccgg ttcatgaaat
     5101 ttttgaatga ttgttcttcg tcggcaaaac tccccgcgcg cttggctagc aagtttttga
     5161 ggttaggaat gacgacgggg tcgatgattt cagcgccggc attttttaat tcgtcaatcg
     5221 ccttgtcgaa cacttcggaa attttattga agtcc
//
LOCUS       DASULZ010000625         9325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_173027, whole genome shotgun sequence.
ACCESSION   DASULZ010000625 DASULZ010000000
VERSION     DASULZ010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9325
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173027"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..1213)
                     /locus_tag="VFY96_12875"
     CDS             complement(176..1213)
                     /locus_tag="VFY96_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5607408.1"
                     /translation="MSPPISEESASEVTGGLWSDVWKRFRRNPIALVGFYVVCFLALV
                     ALLADFLANDKPYYLNYRGKTYFPILRSYLVGLGIAHWPAELLNVDFKRLDGRKIFPP
                     NPYRPGNINLLEPLESPSTKHWFGTDKLGRDVASGMIHGSRVSLSIGFVAVGIAVIIG
                     VMLGAIAGYFGSWVDLVISRLFEIMLSIPTFFLLITVAALLPPSIFLTMAIIGMTGWV
                     GIARFTRNEFLRIRNLDYVTSAVALGVTDRKVMFKHILPNALAPVIVSVVLGIAGAIL
                     VESSLSFLGIGVPAELVTWGSILHEASTTTFAWWLAVFPGFAIFITVLAYYLVGEGLR
                     EVLDPRLRGLR"
     gene            complement(1210..2241)
                     /locus_tag="VFY96_12880"
     CDS             complement(1210..2241)
                     /locus_tag="VFY96_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5607409.1"
                     /translation="MTSYLLQRFLLLVPTFIGITLVGFLTVRLAPGDPAELRAAGGLA
                     AAGGAGISLEKRGAVDEAMAQWRAQYGLDKPLHVQYLVWLKNLFTLNFGESFKDSQSV
                     WGKIAERLPVTIKLNFWSILVVYVVAIPLGIYSATYPNSIADQTTTLIAFILFAVPLV
                     WSATMALVFICGGDFYYLFPPGGLESIAYSGNWPLWRKIADHTWHLFLPVVLLSYDGF
                     AGLSRYMRSSMLDVLGQDYVQVARAKGLKERVVIFKHALRNSLIPQVTILASILPGLI
                     GGSVIIETIFSIPGLGQLGYESVLARDYPTVLALFTVSAVLTLVGILISDLLLAVVDP
                     RIAFGRRQQ"
     gene            complement(2254..4026)
                     /locus_tag="VFY96_12885"
     CDS             complement(2254..4026)
                     /locus_tag="VFY96_12885"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEX5607410.1"
                     /translation="MLRGSWRVGLVLLVVLGCGDKTEVKVADAPSSAIAQNPGKPVTG
                     DWLVAHELSDPEQLNPLTSNDAGSADVLGYIFESLLTRDPKSLELIPALAEARPEISA
                     DKRAYTFKIRRDAHFQDGRPLTGDDVLFSVKAIKCPLVNAPFLRVYFNSLVDAQLLDP
                     YTIRFTIKEPYFLNESVLGGITVMPRHYYDPENLLGKTAVTELLQDPAKLGSNVQKFA
                     GDFNKNYNRNPLGSGPYKFNLWKTGQEVELMRDRRYWGNGKPGIDQPYLDRIKFRVVN
                     NYDAALVRLKSGELDYMERLEPVQVIRGTSSERFTTQFKKYEYFSPTYTYIGWNNDHP
                     IFSDKRVRRAMSYFTDRKQMVKTILFGLGEVVDGPIYFFRPEYDKNLRSIPFDPQKGL
                     ALLNEAGWKDTDGDGILDKTINGRKVPLQFEIKINSGNATRKSIALTLQDELRKYGIA
                     ASVRELDWTIFLDDTKNHKFDAMVGGWQMPVSESDAYQVWHSSQAGNRGSNHISYKNK
                     RVDGILEQYRGEFDEKKRIQLYQEFQEILSDEQPYTFLFCQKWISPVHRRFEDVRVLP
                     GGLRPIEWWVAAANRKYASEMTAN"
     gene            complement(4030..4728)
                     /locus_tag="VFY96_12890"
     CDS             complement(4030..4728)
                     /locus_tag="VFY96_12890"
                     /inference="COORDINATES: protein motif:HMM:NF025490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HEX5607411.1"
                     /translation="MPAPEWEQNKLIDSMSQRVQQFRSIKALAQIDYTGPDGKHGFQE
                     AVLVQRPDRLRLETLSFLGAILIVTANSKELVGYHPREGVFVRGARTKDNLRRYTQIP
                     LELEEITMLLIGLPPVDPKSPVEQVGNTLRFSSKDGKRDVVAFELQQPVPTTWERLNA
                     NGGVELSAEFSDYIETSAGLFPSRIVFEANLQRKKVQIRYQQPEINATISPELFSQQK
                     PANVQEVPIEAIGS"
     gene            complement(4845..6572)
                     /locus_tag="VFY96_12895"
     CDS             complement(4845..6572)
                     /locus_tag="VFY96_12895"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5607412.1"
                     /translation="MLILLLALVGCSTGGPSTQLPADGLGLSPGIPPFRNLPKTEIPS
                     EASAFSHFLMANALLSDGDFDNAVKQMEAAVQAEPNDAFLHFRLASLYVKRGDMRKAL
                     TEAETAARLDPKNVDNHMLLAGLYSSLGENQKGITEYSEALKLDPKNQEALLYLGALY
                     LQIDDLPRATEKLEELLKADPNSMLGHYYLGRVRAKSKLYLAAEQSYRKALELNPKSE
                     AVLLDLALVYELEGKTEDAIQSYQRLLQVNAQNVWARRRLGELYVGQNKLDEALRQFE
                     SLQTSEVDPRETRIKIGLISFEKGDFERAATEFNLVLAGDPDNDRARYYLGATYVEMK
                     AEDKALESFERVSEKSELFLDSRLQVAYLYDRKEQTPKAIDVLQGVLKKGNDRKEIYA
                     FLSSLYRKTKEYPKAIQAMERVVNLDPKNDEAHFQLGALYDENKNKEKSVANMKKAIE
                     LNPKNAAALNYLGYTWAEMGVQLDDAEDLIQRALRIQPNDGFYIDSLGWVYYQKGEYP
                     KAVEYLERAVEITGDDPTIIEHLGDAYERAGRPDRALIRYRDALKLTKETDQSKRLRE
                     KIQRLDKKI"
     gene            6731..8749
                     /gene="ligA"
                     /locus_tag="VFY96_12900"
     CDS             6731..8749
                     /gene="ligA"
                     /locus_tag="VFY96_12900"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX5607413.1"
                     /translation="MTEVSHAAGNEIVRLRRDIEKHNYQYHVLDDPLISDAEYDRLFR
                     RLLELEKKHPELVTPDSPTQKVGGPPLERFSTVQHSLPMLSLNNAMDPQELVEFEERI
                     QRFLKHKESIDYAVEPKIDGLAVEIVYIDGKFTLGSTRGDGITGEDITANLRTVRSIP
                     LMLRADGHAIPKRLEIRGEVFLPLKAFQKMNRDREEQGQPVFANPRNAAAGSLKQLDP
                     TITAKRPLDIFFHGTGIIEGVQFATHCEFRQGLQQWGLKPVPLARLCRGVQEIFRYRD
                     EMEARRDQLLYEIDGLVVKVNSTALQQRLGQIARSPRWAIAYKFKPRQATTRILDIQA
                     NVGRTGTLTPVASLETVPIGGVMVKSASLHNMDEIARKDIRIGDTVVVERAGDVIPYV
                     VKVFTEKRTGDEKQFVMPERCPVCGSEVFREEGEAAYRCIGISCPAQLKESLKFFGSR
                     GAMDIEGLGGKLIDQLVERGLVRDVADLYGLKKEQLAELDRMAAKSAQNLLDNLARSK
                     HTTLARCLTALGIRHVGEATAKLLADHFGELRKIERATEEELMEVRDIGPEVAKSIAR
                     FFAQDANRGVIEKLLAAGVQFRTEPKKAGTLSGKTFVLTGGLDSMSRPEAQKRIEALG
                     GRVTSSVSRSTNYLIAGADPGSKLKKAQEFGAQILSEEEFLQLLEQNQ"
     gene            complement(8743..9063)
                     /locus_tag="VFY96_12905"
     CDS             complement(8743..9063)
                     /locus_tag="VFY96_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX5607414.1"
                     /translation="MGVKLYVGNLPYSVTEEKLQQHFAQHGSVVSARIITDKFSGRSK
                     GFGFVEMSSDQEAEKATNALNGTDFEGRNIVVSEARPQAPRNPRPGGGGGFGSGRPPR
                     RDRY"
BASE COUNT         2174 a         2587 c         2505 g         2059 t
ORIGIN      
        1 aggcgagatg ttgaatgttg ccgggaccgg gcagcgaaac atagatcggc ttgctgattt
       61 gcgcggcgag ggaggacgag caaagtagcg ccgcgacgag ggcgcagaga tgaacgcgtt
      121 tcatgtgact ttctccgcga gtgccgtggc tacccgacta acagaaacgg cgcaactagc
      181 gcaagccccg caaacgcgga tcaagcactt ctcgaagccc ctcgccgacc agataatagg
      241 cgagcaccgt gatgaaaatt gcaaacccgg gaaacaccgc cagccaccag gcaaaggtcg
      301 tggtgctggc ttcatgcaag atcgaacccc aggtgacgag ctcggcgggc acgccgatgc
      361 ccagaaaact cagactggat tcgaccagaa ttgccccggc gatcccgagc acgaccgaaa
      421 cgatcaccgg ggccaaggcg ttgggaagaa tgtgcttgaa catcactttg cgatcggtga
      481 cgccgagggc cacggcggaa gtgacataat ccaagtttct aatccgtaaa aattcgttcc
      541 gggtaaaccg cgcgattccg acccaaccgg tcatcccgat aatcgccatg gttaaaaaaa
      601 tgctcggcgg cagcaatgca gcaaccgtga tcaaaagaaa aaaggtcggg atcgacagca
      661 tgatttcgaa caggcgggag atcacgagat cgacccagga gccaaagtaa ccggcgatcg
      721 ctcccagcat cacgccgata atgaccgcga tgccgaccgc cacaaagccg atcgatagcg
      781 agacccgcga gccgtgaatc atccccgatg ctacatcgcg tccgagcttg tcggtgccaa
      841 accaatgttt cgtcgaaggg gattcgagcg gttcgagcaa attgatgttg cccggccgat
      901 aagggttagg tggaaagatt ttcctaccgt cgagtcgctt gaaatcgaca ttgagcaatt
      961 ctgccggcca atgggcaatt cccagaccga ccaaatagct gcgcaagatc ggaaaataag
     1021 ttttgccgcg atagttgagg taatacggct tatcgttggc gagaaaatcc gcgagcagcg
     1081 caaccagtgc cagaaaacaa acgacataaa accctacgag cgcgatcgga ttacgcctga
     1141 agcgcttcca cacatcggac caaagcccac cggtaacttc actggctgat tcttcggata
     1201 tcggcggact cattgctggc ggcgtccaaa agcgatgcgc gggtcgacga cggcgagaag
     1261 caagtctgaa atcaaaatac ctactagagt cagaaccgcg ctcactgtaa atagcgcgag
     1321 cacggtggga tagtcgcgcg ccaaaaccga ctcgtatccg agctggccaa gaccggggat
     1381 ggaaaagatc gtttcgataa tcacgctgcc gccgatcagc cccggtaaaa ttgacgccag
     1441 aatcgtgacc tgagggatga gcgaattgcg cagtgcatgc ttaaaaatga cgaccctttc
     1501 ttttaggccc ttggcacgcg cgacctggac atagtcctgg ccaagcacgt ccagcatgct
     1561 cgagcgcatg tagcgggata agccagcgaa accatcgtat gagagcagaa ccaccggaag
     1621 aaataaatgc caggtgtgat cggcgatttt ccgccagagc ggccagtttc cggaataggc
     1681 gatggactcg aggccacccg gcggaaaaag atagtaaaaa tcgccgccgc aaatgaacac
     1741 cagcgccatc gtcgccgacc agaccaacgg cacggcaaaa agaatgaaag cgatgagcgt
     1801 ggtagtctga tctgcgatcg agttaggata cgtcgccgaa taaattccca acggtatggc
     1861 cacgacatac accaccagga tcgaccaaaa gtttaacttg atcgtgaccg gcaggcgctc
     1921 ggcgattttg ccccagaccg actgactgtc tttgaacgat tcgccgaaat tgagcgtgaa
     1981 aaggttcttg agccaaacca gatattgcac atgcagcggc ttatcgagcc catattgcgc
     2041 ccgccactgt gccattgctt cgtccactgc gccgcgtttt tcgagtgaaa ttccggctcc
     2101 acccgcggcc gcgagacctc ctgcggcgcg cagttccgcc ggatcgccgg gtgccaggcg
     2161 aaccgttaaa aagcccacca gagtaatgcc gataaaagtt ggaacgagca gcaaaaaacg
     2221 ctgcaataga taggaggtca tcaggcgttt gcgctaattg gcggtcatct cgctcgcgta
     2281 cttccgattc gccgcggcaa cccaccactc gatcgggcgg agcccgcccg ggagaacccg
     2341 cacgtcttcg aatcttcggt gcactggaga aatccacttc tggcagaaca ggaaagtata
     2401 aggctgctca tcactcaaaa tttcctgaaa ctcctgataa agctgaatgc gctttttctc
     2461 gtcgaactcg ccccgatact gttcgaggat cccgtccact ctcttgttct tgtaactgat
     2521 gtggttcgag ccccggttcc cggcctgcga cgagtgccag acttggtacg catcggattc
     2581 gctaaccggc atttgccacc cgcccaccat ggcgtcgaac ttgtgattct ttgtatcgtc
     2641 gagaaagatc gtccagtcta gctcgcgcac gctcgccgcg atgccgtatt ttctgagttc
     2701 atcctgcagg gtcagcgcaa tgctttttcg tgttgcatta ccggaattga ttttgatctc
     2761 gaactgtaaa ggcaccttgc ggccattgat cgtcttgtcc aaaatcccgt ccccgtcagt
     2821 atccttccac cccgcctcat tcagaagcgc aaggcccttc tgaggatcga agggaatgct
     2881 gcgcaaattc ttgtcatact cgggccgaaa gaaataaatc ggaccatcga cgacttcccc
     2941 gagaccgaac aaaatggttt tcaccatctg cttgcgatct gtgaaataac tcatcgcccg
     3001 acgcacgcgc ttgtcggaga agatcggatg atcgttgttc cagccgatgt aggtgtaggt
     3061 cggcgaaaaa tattcgtact tcttgaactg cgtcgtgaat cgttcactgc tggtgccgcg
     3121 gatcacttgc accggctcca aacgctccat gtagtcgagc tcgccgcttt tcaatctgac
     3181 caatgccgca tcatagttat tgacgaccct gaacttgatc cggtccaaat agggttgatc
     3241 gatgcccggt ttgccgttcc cccaatagcg acgatcgcgc atcaactcga cctcttgtcc
     3301 ggttttccat aggttgaact tgtaaggacc gctccccagc gggtttcgat tgtaattttt
     3361 attaaaatca ccggcgaact tctggacatt gctaccaagc ttggcgggat cctgtaaaag
     3421 ctccgtcacc gcagtcttgc cgagcagatt ctcgggatcg taatagtgac gcggcatcac
     3481 cgtgattcct cctaatacgc tttcatttaa aaaatagggt tccttgatcg tgaagcggat
     3541 cgtatacgga tcgagcaact gagcatcgac cagcgaattg aaatagacgc gcagaaacgg
     3601 tgcgttgacc agcggacact tgatcgcttt cacgctaaac agcacgtcat caccggtcag
     3661 tggacgacca tcctggaagt gcgcatcgcg cctgatttta aaggtgtaag ctcgcttgtc
     3721 tgcggagatt tccggacgcg cttcggcgag ggctggtatc agctcgaggc ttttgggatc
     3781 gcgtgtgagc aggctttcga agatatagcc gagaacgtcc gcggagccgg cgtcattact
     3841 agtgagtgga ttgagttgct ccggatcgct cagttcatgg gcgaccagcc aatctccggt
     3901 cactggtttc cccggatttt gcgcgatcgc cgaggatggc gcgtcggcta cttttacttc
     3961 ggtcttatca ccgcagccaa gcactaccag cagcaccaac ccgactcgcc agctccccct
     4021 caacatggct tagctaccga tggcttcgat cggcacttcc tgaacattgg ctggtttctg
     4081 ttgggaaaaa agctcgggag aaattgtcgc attgatctcg ggttgttgat agcgaatctg
     4141 aaccttttta cgttgcaagt tcgcctcgaa aacgatccgg gatggaaaca agccggcgga
     4201 agtctcgatg tagtcactga attccgcgct gagctcaacg ccgccattgg cgttcaagcg
     4261 ctcccacgtg gtcgggaccg gctgttgcaa ctcgaaagcg acaacatcgc gcttgccgtc
     4321 tttactggaa aacctcagcg tatttccgac ttgctctacg ggggacttgg ggtccaccgg
     4381 cggcaaaccg atcagcagca tcgtgatttc ttccagctcg agcggaatct gagtgtagcg
     4441 gcgtaaattg tccttggtcc gagcgccgcg cacgaagacg ccctcgcgcg gatgatagcc
     4501 aaccagttct ttgctattcg cggtaacgat caggattgcc cctaggaagc tgagagtttc
     4561 gagacgaagc cgatccgggc gctgcaccaa tacggcttct tgaaagccgt gtttgccgtc
     4621 aggtcccgta taatcgatct gggccaaggc cttgatcgaa cgaaactgtt gaacgcgctg
     4681 agacatggag tcgatcagct tgttctgttc ccactccggc gcaggcaatt tgggctgcgg
     4741 cggactcgtg actgtcgcac atccggcgaa cccgagagca gtggcgagga ttgaggcttt
     4801 gaccagtttt gaggatctgg tgaaacggct cggaaatcgc acgatcaaat ctttttgtcc
     4861 aggcgctgaa tcttttcacg aagccgtttg ctttggtcgg tttccttggt gagtttgagc
     4921 gcgtcgcgat agcgtatcag agcgcgatcc ggccttccgg ccctttcata cgcatcccca
     4981 aggtgctcga tgatcgtcgg atcgtcgcca gtgatttcca ccgcccgctc gagatattcg
     5041 accgctttag ggtactcgcc cttttgatag tagacccagc ccaaactatc gatgtaaaag
     5101 ccatcattgg gctgaatccg aagcgcccgc tgaatgagat cttccgcatc gtcgagctgc
     5161 acgcccattt ccgcccatgt gtagccgaga taattcaacg cggcggcatt cttgggattg
     5221 agctcaatcg cttttttcat gttggcgacc gatttttctt tgtttttgtt ctcgtcatag
     5281 agcgcgccga gctgaaaatg cgcttcatcg ttcttcggat ccaaattgac gacacgctcc
     5341 atcgcctgaa ttgcctttgg atattccttg gtttttcggt aaagcgagct cagaaatgcg
     5401 tagatttctt ttcggtcgtt gccttttttc agtactcctt gcaatacatc gatcgccttt
     5461 ggggtctgct cctttcggtc atagagatag gcgacttgca aacgggagtc gagaaaaagc
     5521 tcacttttct ccgatacccg ttcgaaggac tccaacgcct tatcttcggc tttcatctcg
     5581 acgtaagtgg ccccaagata atagcgcgcc cgatcattat caggatcacc tgccagaacc
     5641 aggttgaact ccgttgccgc gcgctcgaaa tctccctttt caaatgagat caggccgatt
     5701 ttaatccgcg tctcgcgcgg gtcgacttcg ctcgtttgca aactttcgaa ctgccgcaac
     5761 gcttcatcga gcttgttctg accgacataa agctcgccca gccgacgacg cgcccagaca
     5821 ttttgcgcgt tcacctgcaa gagccgctgg tagctttgta tggcgtcttc cgtcttgccc
     5881 tccagttcgt aaaccagcgc gaggtccaac aacaccgctt ccgattttgg attgagctcc
     5941 agcgctttgc ggtagctttg ttcggcagcg agatagagtt tgctcttcgc gcgcacccgg
     6001 ccgaggtagt agtgccccaa catcgaattg ggatcagctt tgagcaactc ttcgagtttt
     6061 tccgttgctc gaggcaagtc atcgatctgc aggtagagcg cacccagata caggagtgcc
     6121 tcctgatttt tcggatcgag cttcagggct tcactgtatt cagtgatgcc tttttggttc
     6181 tcgcccagcg aagaatagag ccccgccagc agcatatgat tgtcgacatt tttcggatcg
     6241 aggcgcgcag cggtctcagc ttccgtcagt gctttgcgca tatcgcctct tttgacgtag
     6301 agcgaggcta aacgaaaatg caggaaggcg tcattcggct cagcctgcac cgccgcttcc
     6361 atttgcttga cggcattatc gaagtcaccg tcgctgagca gcgcgttcgc cataaggaaa
     6421 tgactgaacg cactcgcttc cgacggaatc tcagtttttg gaagattacg gaaaggcgga
     6481 atccccggag ataaccccaa accatcggca ggaagctgcg tgctcgggcc tcctgtgctg
     6541 cagccgacca aagccagcag caaaataagc atccaaatcg cacgcgtacc aatcatcaac
     6601 tgaagggtta acacaacgct tttggacggt caacgcgcga tttttctcaa gcttcattcg
     6661 ttattgcgac tgtgttagtt tttttcttgc aataggacac ggtgcgtctc aatgatcgaa
     6721 aagctgagct atgactgaag tttcccatgc cgccggcaac gaaattgtgc ggctccggcg
     6781 tgacattgaa aaacataact atcagtatca cgttctcgac gatccgctga ttagcgatgc
     6841 cgaatacgat cgcttgtttc gtcgcctgct cgagctggaa aaaaaacacc ccgagctggt
     6901 gacgccggat tcgccgacgc aaaaagtggg cggaccgcca ctggaacggt tttccacggt
     6961 ccaacactcc cttcccatgc tatcgctcaa caatgcgatg gatccgcaag aactggtgga
     7021 gttcgaggaa agaattcagc ggtttttgaa gcacaaggaa agcatcgact acgcagttga
     7081 accgaagatc gatggtctgg ccgtggaaat tgtttacatt gacggcaaat ttacccttgg
     7141 ctcgacccgc ggtgacggga ttaccggcga ggacatcact gccaatctca gaacggttcg
     7201 ttcgatcccg ctcatgttgc gcgccgacgg ccatgcgatc ccgaagagat tggaaatccg
     7261 tggcgaagtt ttcctgccgc tcaaagcttt tcaaaagatg aaccgggacc gggaagaaca
     7321 gggtcagccg gtttttgcca atccacgaaa tgcggcggct ggttcgctaa aacaactcga
     7381 tccgacgatc acggccaaac gcccgctgga tatttttttc cacggtaccg gcattattga
     7441 aggtgtgcag tttgccaccc attgcgagtt tcgccaaggg cttcagcaat ggggtctcaa
     7501 acccgtcccg ctcgcgcgtc tctgtcgtgg cgtacaggaa atttttcgtt accgcgatga
     7561 gatggaagcg cgccgtgacc agttgctgta tgagatcgat gggctcgtcg tcaaggtcaa
     7621 tagcacagct ctgcagcagc gtctggggca gatcgcccga tcgccccgct gggcgatcgc
     7681 ctataagttc aagccgcgcc aggccacgac gcggattctc gatattcaag cgaacgtcgg
     7741 cagaaccggc acgctgacgc cggtagcgag tctcgaaact gtgccgatcg gcggtgtcat
     7801 ggtcaaaagc gcctcgctgc ataatatgga cgagatcgcc cgcaaagaca ttcgcattgg
     7861 cgatacggtc gttgtcgaac gcgccggcga cgtcatcccc tacgtcgtca aggtgttcac
     7921 cgaaaagcgt acaggtgatg agaagcaatt tgtcatgccc gagcgttgtc cggtttgcgg
     7981 ctcggaagtt tttcgcgaag agggcgaggc tgcttaccgc tgcatcggca tttcatgccc
     8041 ggcacagctc aaagaaagct tgaaattttt tggttcacgc ggagcgatgg atattgaagg
     8101 actcggcggg aaactaatcg atcagctggt cgagcgcggc ttggtgcgag atgtcgccga
     8161 tctgtatgga cttaagaaag agcaactcgc cgagctcgac cgcatggccg cgaaatcggc
     8221 gcaaaatctt ctcgacaatt tggcgcgcag caagcataca acactggcgc gctgcctgac
     8281 cgcgctcggc atccgccatg tcggtgaggc cacggctaag ctcttggcgg accattttgg
     8341 agagctacgg aaaattgaac gcgcaaccga ggaagaactg atggaagtgc gcgacatcgg
     8401 tccagaagtt gccaaaagca ttgcacgctt ttttgctcag gacgccaatc gaggcgtcat
     8461 cgaaaaactt ctcgccgcgg gcgtgcaatt tagaaccgag ccgaaaaaag ctggaactct
     8521 cagcggaaag acgtttgtgc tgaccggggg gttggacagc atgtcccggc ccgaggcgca
     8581 aaaacggata gaagcgcttg gtgggcgagt aacatccagc gtgagccgca gtacaaacta
     8641 cttgattgct ggcgctgacc ccgggtcaaa gctaaaaaaa gcccaagaat tcggagcaca
     8701 aatcctcagc gaagaggagt ttttgcagct actcgagcaa aatcaataac gatcacgccg
     8761 cggcggccgg ccactgccga agccaccacc gccgccgggt cgaggattgc gcggagcttg
     8821 cgggcgagct tcactcacga caatgttgcg gccttcgaaa tcggttccgt tgagcgcgtt
     8881 ggtcgctttc tctgcttcct gatcggagct catttccaca aagccgaagc ccttcgaccg
     8941 gccgctaaat ttgtccgtaa tgatgcgcgc gcttaccacc gagccatgct gcgcgaagtg
     9001 ttgctggagc ttttcctctg tcacgctgta gggaaggttc cccacatata atttcactcc
     9061 catctcgccc tccttgttga atgcaacaac cgctcatttt cacgattcaa acttagccaa
     9121 agggcaagac cttaatgggc gcgcatcagt tgctcggcgc tggtgataac tgtagccctg
     9181 gcgaaacggc gacagcctac aaaagtcgct cctggcttgt caaatctttt gcgcatgggg
     9241 ccgctgctac aggattgcag ggggaccata cattggctaa gaaaccagga tggctgtaaa
     9301 cgcttcagaa actatacgcg ctcgg
//
LOCUS       DASULZ010000626         2559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_579623, whole genome shotgun sequence.
ACCESSION   DASULZ010000626 DASULZ010000000
VERSION     DASULZ010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579623"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VFY96_12910"
     CDS             complement(<1..435)
                     /locus_tag="VFY96_12910"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015121982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEX5607415.1"
                     /translation="MFIILTGVAGAGKTTIGTLLARELGWRFYEGDDFHPAANIEKMR
                     RGEALTDSDRAPWLGRLRALIEQALEREENGILACSALKRSYRARLRVNERVIFVHLA
                     LSRTLIERRLKQRPDHFMNPVLAESQFEALEPPQTGLTLDASL"
     gene            complement(490..663)
                     /locus_tag="VFY96_12915"
     CDS             complement(490..663)
                     /locus_tag="VFY96_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607416.1"
                     /translation="MKNFFWLILITFFGLVCRSAGAAEFQAGQCVQAENDGFMMLLGL
                     FFDEFKFRGTRSG"
     gene            859..1986
                     /locus_tag="VFY96_12920"
     CDS             859..1986
                     /locus_tag="VFY96_12920"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5607417.1"
                     /translation="MAKDGFKIIDSDMHVMEPPDLWERYIDAKFRALAPRGVISHNVR
                     DLRLVHPDGTEWARKSTPQNDTARGKNFDQKQDIFGRDAARGWTSEVQLEAMDTEGID
                     VAVLYPTRGLRALVLPNMDADFAAAMARAYNDWLYDFCQREPNRLIGAGMVSTYSMED
                     AVSEARRCAEQLGFRAIFLRANPLVEHQWHSNYYDPLWDALERLNLSLGFHESTGTGR
                     NPIGERLEPNFMLRRVFSQPMEQMLALGCFCGGGVLARHPKLRVAFLEANCSWLPWLL
                     WRLDEAWELDGDVFAPDLQQKPSDYFKRQCRVSIEPDEVIASNVIEQVGSNYLVFSTD
                     YPHVDSRYPEAVNYFLRLPLSEENKRKILWDNCRSYYAMAA"
     gene            2065..>2559
                     /locus_tag="VFY96_12925"
     CDS             2065..>2559
                     /locus_tag="VFY96_12925"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5607418.1"
                     /translation="MTQFDPVRVGLIGAAGRWGPRAHVPALQGISEAKLYAVCTAHEE
                     TARAAADQFGVARAYGSDDGLNADAEVEAVAIAVRVPAHYALTKHALEAGKHVFCEWP
                     LGANTKEAEELAALARKKNLRTVVGLQRRASPAYLYMRELIQQGYVGQVLSVNLLLMN
                     SGVLT"
BASE COUNT          576 a          718 c          751 g          514 t
ORIGIN      
        1 taaggaggcg tccagagtga gcccggtttg cggcggctcc agcgcctcaa attggctttc
       61 tgccagcacc gggttcatga aatggtctgg gcgctgtttc agccggcgct cgatcagagt
      121 tcgactgagc gccaggtgaa caaaaatgac gcgctcgttc actcgcagcc gggcgcgata
      181 agaacgcttc aaggcggagc aggcgaggat gccgttttct tcacgttcca atgcttgctc
      241 aatcaaagca cgcaaccggc cgagccaagg tgctcgatcg ctatcggtta acgcctcacc
      301 tcggcgcatc ttttcgatgt tggccgccgg gtgaaaatcg tccccttcat aaaaccgcca
      361 gccgagttct ctggccagca atgtacctat tgtcgttttt cctgcgccgg cgacacccgt
      421 caaaataata aacatcgcgc cttagcgccg aaatgggatt gcttcttcag accggcgagc
      481 caggaaattt catccactcc tagtgccccg gaacttaaat tcgtcgaaaa aaagtccgag
      541 cagcatcatg aagccgtcgt tctcggcttg cacacactga cccgcctgga attccgcggc
      601 gccggcggac cggcaaacaa gcccaaaaaa cgtgatgaga atcagccaaa agaaattttt
      661 catcgattga cgcggtacaa cagcgatcat aggagagctg ccgacgcact gtcaacgcgg
      721 caatacagga aaccgccttt cttcagccat ttcgagctgc gactgattga cgcgccgcgg
      781 cgggagcgat agcgtaaacg ggaaataaca actagcgtta cagtcgtctg agcacaacca
      841 aaatcgaagg aatcagtcat ggccaaagat ggattcaaaa tcatcgacag tgacatgcat
      901 gtcatggagc cgccggattt gtgggagcgc tatatcgatg cgaaattcag agcgttggcg
      961 ccgcgcggcg tcatcagtca taacgtgcgg gatctccggc tcgtgcatcc ggacggaacc
     1021 gaatgggcgc gcaaatccac accgcaaaac gataccgcca ggggtaaaaa cttcgatcag
     1081 aaacaggaca tcttcggacg cgacgctgcg cgcggctgga cttccgaagt tcagctcgaa
     1141 gcgatggata ccgaaggtat cgatgtcgcc gtgctttatc ccacgcgcgg tctgagagcg
     1201 ctggtgctgc cgaacatgga tgctgatttc gccgctgcca tggcgcgcgc ctataatgat
     1261 tggctctacg atttttgtca gcgagagccg aaccgtttga tcggcgccgg aatggtttcc
     1321 acctacagca tggaagatgc ggtgagcgaa gcacgccgct gcgccgagca gttagggttt
     1381 cgggcgattt ttctccgcgc caatccgctc gtcgaacatc aatggcacag caactattat
     1441 gatccactct gggatgctct ggagaggctc aacctatcgc tcggttttca cgagtccacc
     1501 gggacgggga gaaatccgat cggcgagcgc ttggagccca atttcatgct gcgccgggtt
     1561 ttttcccagc cgatggagca gatgctcgcg ctcggctgtt tttgcggcgg cggcgttctg
     1621 gcgcgtcatc caaaattgcg ggtggcgttt ctcgaagcga attgcagctg gctgccctgg
     1681 cttttatggc ggctcgacga agcgtgggag ctcgacggcg acgtcttcgc tccggactta
     1741 cagcaaaaac cgagcgatta tttcaaacgg caatgccgag tgtccatcga gcccgacgag
     1801 gtgatcgcca gcaatgtcat cgagcaggtc ggcagcaact atctggtttt ttcgacggac
     1861 tatccgcacg tcgattcgcg ctaccctgaa gcggttaatt atttcctgcg cctgccgctg
     1921 agcgaagaaa acaagcgcaa gattctctgg gacaactgca gatcctatta tgcgatggcc
     1981 gcatgacgga acggaccggg tacgctctgc gcatttggcc aaacgatgtc tacgcatcga
     2041 tccggagagt gactgagggg aaccatgaca cagttcgatc cagtacgagt cgggctcatc
     2101 ggagcggccg gtcgatgggg gccgcgagcg catgtgccgg cgttgcaggg catctcggaa
     2161 gccaaactct atgccgtctg cactgcgcac gaagagacgg cgcgggcagc cgccgatcaa
     2221 ttcggcgtgg cgcgcgccta cggcagcgac gacggattaa atgccgatgc cgaagttgaa
     2281 gccgtcgcca tcgcggtgcg tgtgccggcg cactatgcgc tgaccaaaca cgctcttgaa
     2341 gcgggaaagc atgttttctg cgaatggccg ttgggcgcga atacgaaaga agccgaagag
     2401 ctggcggcgc tggcgaggaa aaaaaacctg cgcaccgtcg tcgggctgca gcgccgcgcg
     2461 tcgcccgctt atctgtacat gcgtgaactc atccagcaag gttacgtcgg acaagtcctg
     2521 tcggtgaatc tgttgctgat gaacagcggg gtattgacc
//
LOCUS       DASULZ010000627         3925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_579656, whole genome shotgun sequence.
ACCESSION   DASULZ010000627 DASULZ010000000
VERSION     DASULZ010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579656"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VFY96_12930"
     CDS             <1..309
                     /locus_tag="VFY96_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607419.1"
                     /translation="DEGAPVETILFDPVLSNPAYLLLTKNSSHPHAAALLIDWALSAD
                     GGMKFFAEKFGRTPTRTGLKERFPTLRVNNYLVVKPEIVGPNFKEFTNLYNEIFGIGK
                     "
     gene            792..1418
                     /gene="nthA"
                     /locus_tag="VFY96_12935"
     CDS             792..1418
                     /gene="nthA"
                     /locus_tag="VFY96_12935"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEX5607420.1"
                     /translation="MAKHDHDHIEGSNLSEMDLRVRALETILVEKGYIESAALDRIVE
                     LYETKIGPHIGAQVIAKAWSDLVFRQALLDDASKAIAAFAPHVGSVGDHLIAVENTPE
                     VHNMIVCTLCSCYPTDVLGLPPTWYKSFAYRSRAVKEPRAVLADFGVTLPVETEIRVW
                     DSTAETRYLVVPMRPAGTEGWSESQLAKLVTRDSMVGTGLAKKPSEVA"
     gene            1418..2077
                     /gene="nthB"
                     /locus_tag="VFY96_12940"
     CDS             1418..2077
                     /gene="nthB"
                     /locus_tag="VFY96_12940"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HEX5607421.1"
                     /translation="MDGVHDMGGMDGFGKVEPEANEPVFHHRWEGRVLAMSRAIGVFG
                     AWTIDTSRYAIELLSPSVYLTRSYYEKWFLRNQNLLIERGLIDTDEVAAGRALRPAKN
                     LTRGPFTLADVEKVTRRGSYGRPAPAVAQFKVGDRVRTKNIHPATHTRLPRYARGRVG
                     VVERLHGTHIFPDSAAVGKGDDPQWLYTVRFDAQELWGPDAEPKLKVSIEAFEPYLER
                     A"
     gene            2070..2474
                     /locus_tag="VFY96_12945"
     CDS             2070..2474
                     /locus_tag="VFY96_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HEX5607422.1"
                     /translation="MPESPPIEPEAVRRAAEAIPGIPRDDNGPIFRAPWEAHAFAMAL
                     ALYQKGLFAWSEWSAMLGEEIKKAQAAGDPDTGETYYLHWLATLERMVAEKGAATAQA
                     LTQHYQAWERATHRTPHGRPIELKPEDFVSDG"
     gene            2777..3754
                     /locus_tag="VFY96_12950"
     CDS             2777..3754
                     /locus_tag="VFY96_12950"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX5607423.1"
                     /translation="MLKLKLTLACWDYDRTRPIIDGRVQAEGIDLEIKVMRPREIFPR
                     MLEDKEFEVSELSLGSYVSLIGRGGCPFVAVPIALSKIFRHSCIYVRTDAGIRTPQDL
                     RGKRVGTTQLGSTATIFMNGMLKHEHGVGMEDMQWFIGGMTAPTQRPLIPLKLPDKVK
                     VEFLPPERTLEGMLENGDLDALLAIFIPSIFEKRSPRIARLFPNYYEMERDYYRRTGI
                     FPIMHTVVLRDDIHREHPWAARSIYKAFCEARDLAVEGLYDTDALRLSLPWLIHHVEE
                     TWRVLGKDFWSYGLEPNRPALKAIGEYVYEQGLSPRAVCPDELFAANVE"
BASE COUNT          849 a         1083 c         1139 g          854 t
ORIGIN      
        1 gacgaaggcg ctccggtgga aacgatcctt tttgatcccg tgttgtccaa ccccgcttat
       61 cttctcctga cgaaaaattc ttctcatcct catgccgccg cccttctgat cgactgggcg
      121 ttgtcggccg acggcggaat gaagttcttc gccgaaaagt tcggtcgcac gccgacccga
      181 acaggtttga aggagcgctt ccccacactg cgcgtaaaca actacctggt ggtcaagccc
      241 gagatcgtcg gcccgaactt caaagaattt accaacctgt ataatgaaat ctttggaatc
      301 ggaaagtagt caatcggcag caaaaagaac atacgaaaat ccgacacacc gctgttgcgc
      361 gaacgggaca agttaatgaa gcagctgttt ggcagacgat aacacctgcg acacagttgt
      421 tcgggtttcg cagaaacttt atgaagaaat cctccgtcca ttggaatcgc gagtcgcttt
      481 tttagctttt gttgcggtgg cctgcacgtc gggttggatt cagcctgccg gaaatagttg
      541 gtgtcggttt cgggatagtt gaactttctc gctttcaggt gaagtaaaaa tttgtcatgc
      601 ccgcgccaac atccggttta ccgtctgttc agaattaaga gccggtatgc gccgaatgac
      661 tggaaccgga gttcagttca actcgacaga ctacgaacac ctcccgttag agtggagcgg
      721 tcagacacca cagcacccgg cgttcgattc gccgattgca cgatgccgct ttcgttgggt
      781 atattttgcg tatggctaaa catgatcacg atcatattga gggttccaat ctttccgaga
      841 tggatctacg ggtgcgcgcg ctcgaaacga tactcgtcga gaagggctat atcgaaagtg
      901 ccgcgctcga tcgcatcgtc gagctttatg aaaccaagat cggcccgcat attggcgcac
      961 aggtgatcgc gaaggcatgg agcgatcttg tctttcgcca ggcgctgctc gacgacgcaa
     1021 gtaaggccat tgctgccttc gcaccgcacg tcggaagcgt gggcgatcac ttgatcgcgg
     1081 tcgaaaatac gccggaggtg cacaacatga tcgtgtgcac gttgtgctcc tgttatccga
     1141 cggacgtgct cgggctgccg ccgacttggt ataaatcgtt cgcttaccgc tcgcgcgccg
     1201 tcaaagagcc acgcgcggtg ctggcggatt tcggcgtcac actgccggtc gaaaccgaaa
     1261 ttcgcgtttg ggactctacc gccgaaacgc gctacttggt cgtgccgatg cgaccggcag
     1321 ggaccgaggg ctggagcgaa tcgcagcttg ccaagttggt cacgcgcgat tcgatggtcg
     1381 gtaccggcct ggcgaaaaaa cccagcgagg ttgcctgatg gacggcgtcc acgatatggg
     1441 cggcatggat gggtttggca aagtcgagcc cgaggctaac gagccggtat ttcatcatcg
     1501 ctgggaagga cgcgtgctgg ccatgagccg cgcgatcggc gtctttggcg cctggaccat
     1561 cgataccagc cgttatgcga ttgagttatt atcgccgtct gtctacttaa cgcgctcgta
     1621 ctacgagaaa tggtttctgc gtaatcaaaa cctgctgatc gagcgcggtc tcatcgatac
     1681 ggatgaagtc gccgccggac gcgcgctgcg ccccgctaag aatttaaccc gtgggccgtt
     1741 cacgcttgcc gatgtggaaa aagtcacgcg gcgcggctcc tatggacggc cggcgccggc
     1801 ggtggcgcaa ttcaaagtcg gcgatcgcgt gcgcacgaaa aacattcatc cggcgacgca
     1861 cacgcgtttg ccgcgctacg cgcgcggccg tgtcggcgtc gtcgagcgcc tgcacggcac
     1921 gcacatcttt cccgactccg cagcggtcgg aaagggcgac gatccgcagt ggctttacac
     1981 ggtgcgcttc gatgcccaag agctttgggg tccagatgcc gagccgaaat tgaaggtctc
     2041 catcgaagcg tttgaacctt atctcgaacg tgcctgaatc accgcccatc gaacccgaag
     2101 ccgtccgccg cgccgccgaa gccattccag gcataccgcg cgatgacaac ggtccgatct
     2161 ttcgcgcccc gtgggaagcc cacgcgttcg ccatggcgtt ggcgctctat caaaaaggtc
     2221 tgttcgcctg gagcgagtgg tctgcgatgc tcggcgagga aataaaaaaa gcccaagcgg
     2281 ccggcgaccc cgacactggg gaaacttact atcttcattg gctcgcgacg ctggaacgca
     2341 tggtcgcgga aaaaggcgcg gccacggcgc aagctctgac tcagcattac caggcctggg
     2401 agcgcgcgac gcaccgaaca ccgcacggaa gaccgatcga attgaaaccg gaagatttcg
     2461 taagtgacgg atagctccag gacgggtggt gctgctgtag tatttccaaa gctccagcgg
     2521 agcgtgtagc acaagacagt aggaaagcct gaaactcgag cagtgctcat ggggagaaaa
     2581 aaacaatgca agggtttgac acagtctcga ttgacgaacc gtggcttttc catcgacgat
     2641 ctcattcacg atctcttgat cggtcgactg ctggcgattt caatcaccgg atttcttgct
     2701 agctcatcgc agtgtgttac ttctacggac acgtttcaca ttcaaccagg tttttctctg
     2761 gagacaggtt gcaccgatgt tgaaactcaa acttaccctc gcgtgctggg actatgaccg
     2821 cacccggccg atcatcgatg gtcgtgtcca ggccgaaggc atcgatctcg agatcaaggt
     2881 catgcgtcca cgcgagatct ttccgcgaat gctggaggac aaagagttcg aggtttcgga
     2941 gctatcgctt ggctcttacg tgagtctgat aggccgcggc ggttgtccct ttgttgcagt
     3001 gccgatcgcg ctgtcgaaaa tctttcgaca ctcttgtatt tacgtgcgaa ccgatgcggg
     3061 gatccggacg cctcaggacc tccgcggcaa acgggtggga acgacgcagc tcggttcgac
     3121 cgccaccatt ttcatgaacg ggatgctgaa acacgaacac ggtgtgggca tggaggatat
     3181 gcaatggttc atcggcggaa tgacggcgcc gacgcagcgg cctttgattc cgttgaagct
     3241 gccggacaag gtcaaggtcg agtttttgcc gccggagcgg acgctggagg gaatgctcga
     3301 gaacggcgat ctcgatgcat tgctcgcgat ctttatcccc tccatttttg agaaacgctc
     3361 gccgcgcata gcgcgtctgt ttccgaacta ttatgagatg gagcgggact attatcgccg
     3421 caccggcatc tttccgatca tgcatacggt ggtgctgcgc gacgatatcc atcgcgagca
     3481 cccgtgggcg gcccggagca tctataaggc tttctgcgaa gctcgggatc tggcggtcga
     3541 gggactttac gacacggacg cgctgcgcct ttcgcttcct tggttgatcc accacgtcga
     3601 ggagacctgg cgggtgttgg gaaaagattt ctggtcgtac ggactggagc cgaatcggcc
     3661 ggcgctgaaa gcgatcggcg aatatgttta cgaacagggt ctctcgccgc gggccgtttg
     3721 ccctgacgaa ctattcgcag ccaatgtgga gtaatgatgg tccgccaaag ggccaacggg
     3781 gtagaccgaa atatctctac gcctccgaat ccgcactctc atttcattga gcgagagcga
     3841 agcatctgct tttcaagtcg ggaaagcgga ttcttcggtt tcgcctcata atgacacttc
     3901 aggacctcat tttttgaacg gagaa
//
LOCUS       DASULZ010000628         2317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_477823, whole genome shotgun sequence.
ACCESSION   DASULZ010000628 DASULZ010000000
VERSION     DASULZ010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477823"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /locus_tag="VFY96_12955"
     CDS             <1..1220
                     /locus_tag="VFY96_12955"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEX5607424.1"
                     /translation="LQYIDQAIVLLRDAELQDRVCRLLRSLDKMVSIVSLTSEDNGHH
                     KETPDPLLRHLQLSDIFEEFCASQLLDTLNVKKVERIRSLFSAGKKIHILLQHDPDPD
                     AIGSALALRELLGRNRPTTPIVTFGEITRPENLAMIRLLDIQIDKISYEDLHKDGARL
                     ALLDVQPPYFQQPLGRVDLVVDHHPKRIAFKARFADLRSGYGATSTIFTEYLRAAGME
                     PSQRLATALVYGIKTDTLFLERGSNLADLSAFNFLYPLANKAMIARIERPALPREDLE
                     AMGRALSRLQVDNGVAVIHLGEINREDVIPQLAEFCLQIEGVDWSVVSGLVKDRVVIS
                     VRNVGYVKSAGDIMKRLYDDIGSAGGHRAMAKAVVPLDRFKQRFGEVSEKVIREAMLP
                     MIVDRDQIELELH"
     gene            1317..1709
                     /locus_tag="VFY96_12960"
     CDS             1317..1709
                     /locus_tag="VFY96_12960"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5607425.1"
                     /translation="MPKLRHLAIRTEDTGKLASFYMDVFEMQMLHKDKNEGGAIFLTD
                     GYFNLAILPNHEQQAPNGLYHFGFEVESGEKIVERLKKINPNKLPKPRPNGRPYAETR
                     GSDPDGNYFDISEHGFQNVVPLGEKKEN"
     gene            1784..>2317
                     /locus_tag="VFY96_12965"
     CDS             1784..>2317
                     /locus_tag="VFY96_12965"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607426.1"
                     /translation="MRCRRHHGLGFWLPPASCAAIALALALVTAERIAAQTKSNFASS
                     VTSESMAAVWVARDRGLFKKYGLDAQFILMPRNPLAVAALLAGEIDTAIIGPGHLLNA
                     GLSGADLIGIANFHLKLDYRLNVRPEIKKAEDLRGKRIAISGPGSTSHLVSLLALQGL
                     NLDPVQAHITFLTISGTE"
BASE COUNT          504 a          650 c          646 g          517 t
ORIGIN      
        1 agctccaata tattgaccaa gcgatcgtct tgttgcgcga cgccgaactg caagaccgcg
       61 tctgccgttt gcttcgctcc ctcgacaaaa tggtctctat cgtctcgctt acttccgagg
      121 acaacggcca tcacaaagaa acgcctgatc cactgctgcg ccatttgcag ctctccgata
      181 tcttcgaaga attttgcgcc tcgcagctgc tcgatacctt gaacgttaaa aaagttgaac
      241 ggattcgttc gctcttctcc gccggtaaga aaatccacat tctgctgcaa catgatccag
      301 atcccgacgc catcggcagc gcactggcgc tgcgcgagtt gctcggtcgc aaccgtccga
      361 ctacgccgat cgtgaccttc ggtgaaatca cgcggccgga gaatctcgcg atgatccgtc
      421 tgctcgacat ccagatcgac aagatttcct atgaggatct gcataaagac ggcgcccgat
      481 tggcgctgct ggacgtgcag ccaccgtact ttcaacagcc cttggggcga gtcgacctgg
      541 tggtcgatca tcatcctaaa aggatcgcgt tcaaggcgcg ctttgctgat ctgcgcagcg
      601 gctacggcgc cacatcgacg atcttcaccg aatacttgcg cgctgcggga atggagccgt
      661 cgcagcgctt ggccacggct ctggtttatg gcatcaagac cgacacgcta ttcctcgagc
      721 ggggcagcaa tctcgccgac ctgagcgcat tcaatttttt gtatccgctc gccaacaagg
      781 cgatgatcgc gcgcatcgaa aggccggcgc taccgcgcga ggatctcgaa gcgatgggac
      841 gcgccttgag ccggcttcaa gtcgataacg gtgtcgcggt gattcatctc ggtgaaatca
      901 atcgcgaaga cgtgattccg caattggccg aattctgcct gcagatcgaa ggtgtcgatt
      961 ggagcgtagt ctcggggtta gtcaaagatc gagtggtgat ttcggtacgc aatgtcggct
     1021 acgtgaagag tgccggcgat attatgaaga ggctttacga tgacatcggc agcgcgggcg
     1081 gccaccgcgc catggcgaaa gcggtggtgc ccctggatcg cttcaaacaa aggtttggcg
     1141 aagtcagcga aaaagtaatt cgtgaggcga tgctgccgat gatcgtcgat cgtgaccaaa
     1201 tcgagctcga gctacattag cttgccatcc cgtccagcct cggctgctag gctttcttga
     1261 catttccagc ttatcaggac taagggtgaa ctaatttata ttcaaggggg ggttttatgc
     1321 ctaagctgcg ccatctggcg attcggaccg aagacacagg aaagctcgca tcgttttaca
     1381 tggacgtgtt cgagatgcag atgctgcaca aggacaaaaa cgagggcggc gcgatttttc
     1441 tgaccgacgg ctacttcaat ctcgctattt tgcccaatca cgagcagcag gcgcccaacg
     1501 gactgtatca ttttggcttc gaggtcgaga gcggcgagaa aattgtcgaa cggctgaaaa
     1561 agatcaatcc gaacaagctg ccgaagcctc gtccgaacgg ccgtccctat gcggaaaccc
     1621 gcggttctga tcccgacggt aactacttcg atatctccga gcacgggttt cagaacgtgg
     1681 taccgctggg cgagaaaaaa gaaaattagc tgaaccggtg tgctgatgct tacctggcga
     1741 gctgtaccct cgcctcggct gtcaatctaa cgagatcgtg gcgatgagat gccggcgtca
     1801 tcacggtttg ggtttctggt tgccgcccgc tagttgcgcc gcgatcgccc tggcgcttgc
     1861 cctggtcacg gccgagcgaa tcgccgctca aactaaatcc aatttcgctt ctagcgtcac
     1921 cagcgaaagc atggccgccg tctgggtcgc gcgcgatcgt gggctattca agaagtatgg
     1981 gctcgatgcg caatttattc tgatgccgcg aaatccgctc gcggtggcgg cgttgctcgc
     2041 cggcgaaatc gataccgcga tcatcgggcc ggggcatctc ctcaacgccg gcttgagcgg
     2101 cgcggatctc atcggcatcg ccaattttca tctcaagctc gattatcgtt tgaacgtgcg
     2161 acccgagatc aagaaagcgg aagatttgcg cggcaagcgt atcgcgatca gcggccccgg
     2221 gtcgacctcc catttagtct cgctgcttgc gttacaaggt ttaaatctcg atccggtgca
     2281 agcgcatatt acttttctga cgatttccgg gactgaa
//
LOCUS       DASULZ010000629        11948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_985098, whole genome shotgun sequence.
ACCESSION   DASULZ010000629 DASULZ010000000
VERSION     DASULZ010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 11948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11948
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985098"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..578)
                     /locus_tag="VFY96_12970"
     CDS             complement(201..578)
                     /locus_tag="VFY96_12970"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX5607427.1"
                     /translation="MGIKRGKTFGTERGFTLLELMVVVAILGILMAIAIQQFSLYRSR
                     ATDAAMRSDLKNAALAMESYYGEFLDYPVSVNALRLVGYRNTNGVTLTINVSSPSSFT
                     LNAATPNGTQPSFTFDSTTGLIN"
     gene            complement(705..1796)
                     /locus_tag="VFY96_12975"
     CDS             complement(705..1796)
                     /locus_tag="VFY96_12975"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5607428.1"
                     /translation="MTKNLNLNEAAAFSPKSPYEIYQEWEGIPVYKDFIIPDLLKLEL
                     GDWARTGGKAGFVNMDGSGGTCDILIEEIAPGGQLKPIRHMYEKAIFILEGQGATTIW
                     NEGGKKHTLEWQKGSLFSTPLNTWHQHFNAQGNAPVRMISLTDAPVIVNRYRNLDYIF
                     NNNFTFSDRYSGGADEWGKGGRYIPEVKRGRVWESNFIADLWGFQPKDYKERGGDNRT
                     TLFEFVDNTMSAHLSEFPVGKYKKAHRHGAGAHIVMLTGAGYSYLWPEGEYDNKRRVE
                     WGRMSMFVPPMQWWHQHFNPGPEPARYLALKPWGFKFNVEDLKDTGEDVKKGGAQIEY
                     EDQNPEVHRIFLSECGRRGAQARMPMFGA"
     gene            complement(1864..2568)
                     /locus_tag="VFY96_12980"
     CDS             complement(1864..2568)
                     /locus_tag="VFY96_12980"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX5607429.1"
                     /translation="MNTLGDLLKTLDQMVNDQFESVEFKKFLGVPLTLERGRFYVVQN
                     ALYTSNRRDCWGYVQGGAPLEVKRIIWEHESDELVHDPRAGMDHYALSVKQGEVIGLK
                     PEDFERAEVPSMVRACFFAWHHIAMHNHWLSAYTASHMLERRNNGKIVKGGGMSYRVG
                     KKFENELGINLKRMISLDVHVVADTDHSENISEMFERYVKTPEDCELVLKGARESMAI
                     DRAYRGALGYYMEQIK"
     gene            complement(2602..3324)
                     /locus_tag="VFY96_12985"
     CDS             complement(2602..3324)
                     /locus_tag="VFY96_12985"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX5607430.1"
                     /translation="MKATPDAIDAMWTELVRMCNEQFESKAFRRLLSTKFTKARAQHY
                     SIQMAFYVQNRRDCWGYVQGSAPLEIKKLIWQHEEDELIGRKSEGKQDHITLAVKEGE
                     IFGLTAEAFERNPRLEGGEVCFSAWIRLAQRSWLEAIAASAILEMRNSGELIEGGSLS
                     RRIGDMLERDLGIPMKKQINTAEHVVMDVEHAHLLMQVARKYANTVIARKAILQGAAD
                     SLMIDRVYRGHLADMLEALPDG"
     gene            complement(3707..5182)
                     /locus_tag="VFY96_12990"
     CDS             complement(3707..5182)
                     /locus_tag="VFY96_12990"
                     /inference="COORDINATES: protein motif:HMM:NF018032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HEX5607431.1"
                     /translation="MAIVTHEHHGHGEHGHDHEGAVGPHTHLTSVGIDIGSSTSHLMF
                     SQLLIGYPSVLQRKPIVLERNVIARSPILLTPFSGDWNIEAEPLRELVNQTFQAAGLS
                     REEIDTGAVIITGEAARRDNAQKIAELFSDEAGRFVCATAGPTLETIMAAHGSGAVLH
                     SRDQGITLLNIDVGGGTTKISLIVNGKIRATTALNIGARLIAHDATNTIVRLERAGRQ
                     FLLDLGQNLDLGQNISQDLKARLADRMARGLFDALTTGTAPREDVFITAAAALGELPS
                     IDGVLFSGGVSEYIYSREAHAFGDLGPELGRAIRHEAERRGFNILAAGEGIRATVIGA
                     SQYTVQLSGETIFIPESMTLPARNLRVFVAEIDWQAPVLEKTAAVVARTLGERDPEVR
                     GAPFVIAFSTPPFTGYGAVQEMARGIDRALENLAGDDRPVALVFVHNIGQVVGGMLSG
                     KWNMPCIDEVGLSELDFIDIGEVVESEGFVPVVVKSLAFGV"
     gene            complement(5352..6326)
                     /locus_tag="VFY96_12995"
     CDS             complement(5352..6326)
                     /locus_tag="VFY96_12995"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX5607432.1"
                     /translation="MNNSQKPRLSLAAGYHLRARPLCDGRVKMKDFELDAVPFENDGQ
                     GHDEFLSGKFDTGEFSLANYCALKSRGGPYMAIPVFPNRKFRHSYIFVPENSPLKEPA
                     QLKGKKVGIPGWLNTCGLWVRGILSDEYGVRAQDIHWISTRPAKLDLSLPAGIRIDIV
                     PGQGSLAARMLQGEFDAIIVPDFPEEKGWRRLFADSKAVEQDFFRRTGLFPTSHAITF
                     HSSYLEKYPAAAQAMFEVCLRAKNLALRDDADATYSNFAWNRQVWEEQQSVLGPDPWK
                     FGVKGNEKVLNTLIRYAEEQGLLVKKITVDDLFIQIDEPNGNVLLHTV"
     gene            complement(6436..7293)
                     /locus_tag="VFY96_13000"
     CDS             complement(6436..7293)
                     /locus_tag="VFY96_13000"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607433.1"
                     /translation="MALSVRNVVFLPFYYAKDIKIFEKHGLNVELIQMRSDLQLAGLA
                     SGEIDFTPSVGPAGAAIANGLPIKALAVLYRAPLFSLVSPAGLASVKDLEGKTVAVSR
                     IGSESHRYGSLMIESGGADPKKVTFIQTGSTTVSLTALQQNSVNAAVLSPPFTGIMAE
                     KGYKILARSRSLLQDAPWLGLVASRQKIERQAEQVKNMLRSMRDVIAAIKRERSAVVT
                     YIEKNFKVSAANANESYDDIMGVMVEPLLLRDEQIQKYLDGSFGRGEIPKRLSTAEMF
                     DFALLRGLK"
     gene            complement(7563..8348)
                     /locus_tag="VFY96_13005"
     CDS             complement(7563..8348)
                     /locus_tag="VFY96_13005"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX5607434.1"
                     /translation="MSLKIERGKKYSVAEAKKTVDKLYSLVHERWEANVNQKPFMQQL
                     MAGKLPLKVLQTFFRNWGAYTIEINTLTACTYHRNITFLKVNRDLMGPIGEKIADEFI
                     HPKPPGHFLVMLQTAQAFGLTEEEVFTTPMLPEFRGKIDFMRGIVYEGTAAEWYGAVT
                     TEEQIGHWSAACYQALTTHYGFDRDKATYFATHVEADLAEHEEGVMGHASFNRMALQR
                     LLETGMADERPTYGIEYCAMTSVDLHGVMLRAAMEEAERGIKA"
     gene            complement(8390..9328)
                     /locus_tag="VFY96_13010"
     CDS             complement(8390..9328)
                     /locus_tag="VFY96_13010"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607435.1"
                     /translation="MRPPRVITIALFSILFVAGDGITAQLTKVRISYSSRSNSNTPFI
                     IALNKGFFVEEGMDVELIQVNPRLAATAVINGDLDLTTTFGTTLRSIIGGFPIKFVAV
                     AVKKSEHILVVRPEIKEIRDLNGKKLGVATLFGSDQRAAEEMLKGKGFSPTMMKPIAL
                     GESPVRAQALRAGLVDAISITSPFDLSLKSEGFRFLAGPQDVQIALPTSGLAVSNRTL
                     QQNPQLVKRSVRALLKAHRFVFENRKEVPPQMIRYLDQTPDVAERSYDLLVGSLSRNG
                     EITDQEWDALTEKKKPVDEVRDFTLLREAQKELKIK"
     gene            complement(9363..10469)
                     /locus_tag="VFY96_13015"
     CDS             complement(9363..10469)
                     /locus_tag="VFY96_13015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5607436.1"
                     /translation="MEYYDYVIIGAGFAGAATAYHLTRRGARSVLLLEQESIPGFHSS
                     GRNAAMMRQCVPDPDLAALTRAGGAFLRQFPSDWPEPVEFKQNGSLLLGSGAGWQKLQ
                     HDAAIGRSVGIETELWTPEQAKRCVPILQGAEFEGAAWCATDGIIDIHALLSGYLKAA
                     VRNGARIRYACKVRELRNSSNEISIKVEGETIGAGTVINAAGAWAGVIAKMVGAREMP
                     LHPCRRHLFVSPPLGWVDPSWPFVWDVTHDIYFRPEGEGLLLCACDQTELAPGDPPVD
                     GSVQELLAEKIGEFIPGLSSVSIARGWAGFRTLTPDGRFIIGWDPDVKHFFWVAGLGG
                     HGMTTSSAVGELAAELLLGGADKKSTPFSPDRFV"
     gene            10601..>11948
                     /locus_tag="VFY96_13020"
     CDS             10601..>11948
                     /locus_tag="VFY96_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607437.1"
                     /translation="MRIHRYTEWDGKQQVLFPTTDDLLKHLSDHLLEEEGVRRALRDL
                     MRRGFTSEDGQRSVKGMRDFLREADEKRRELLNKYSPDSFKLTAEEQKTLSEKLNSLA
                     EKLEKYHEQMRNFMERMSGKFADRMDELSEKMQEAYQRYQELRQRLQNQIAERGMQKQ
                     SNMSGQDPQSLMDMLDRMQKLLEDEDFLKNLPSMMDAAVNNLESMLENLDSMTQEQLQ
                     QLSDMMTQMQQLEQLMNQCGFQGSQRMGMGEAGQILGQLRGLERFLRWGQRGMGDPSE
                     LNLDELREILGEEAYENLKYLKGVEQALEDEGYLVRTNHGLRLTPKGMRKIGDKALRE
                     IFQMLNKSRWGNHATSSRGSQGDRMEETKEYEYGDPLHVNIGETLLNALERRKRGEPL
                     RIAPEDFSVHRVEYSTVSETALLIDVSYSMLMNDALHAGKKVALALHRLIQTKYPQD"
BASE COUNT         2712 a         3435 c         3080 g         2721 t
ORIGIN      
        1 atagaagaaa gatttctcgc ggttggctcg aaatgacatg gtgagagcct caaatgcggt
       61 gacgaggctg gaacgaacga tagcagtagt gtgggtggag gcgcagtacg ccggtaccca
      121 ccagtcgatg atggctgtga cggagcctgt cgtaaacaca cccgaaaggg ctcgacccct
      181 cctacgcctt acgaagtatt ttagttgatc aacccggtgg tgctgtcgaa ggtgaaactt
      241 ggctgggttc cattcggagt tgccgcgttt agagtgaagc tcgatggcga actcacattg
      301 attgtcaacg ttacgccatt cgtatttcta tagccgacca gtcgtaacgc attgaccgat
      361 accggatagt cgagaaattc gccgtaatag ctttccattg ccagcgccgc attcttgaga
      421 tcggaacgca tagccgcgtc tgttgcccgg ctccgataaa gcgaaaactg ctgaatcgcg
      481 atcgccatga gtatccctaa tatcgcgacg acgaccatta attcgagcag agtaaagcct
      541 cgctcggtcc caaaagtttt tcccctttta atacccatcg gcaagaacct aaccctgttt
      601 tataccaaaa aatcgacaaa atggcgagaa aaaaattcag agccgcgggt caagccctgc
      661 tttgaaaggg agtcggggtg tcgaatcgct gtccgtttta gccgttacgc accgaacatc
      721 ggcatccgtg cttgcgcccc gcgtcgcccg cactcggaca agaaaattct gtggacctcc
      781 gggttctgat cctcgtattc gatctgagcg ccgccctttt tgacgtcttc tcccgtatct
      841 ttgaggtctt ccacattaaa tttgaatccc cacggcttta gagcaagata tcgtgcgggt
      901 tcaggtcccg gattgaaatg ctgatgccac cactgcatcg gcggcacaaa catgctcatg
      961 cggccccatt cgacgcggcg cttattgtcg tattcgcctt ccggccaaag gtaggaatat
     1021 cctgcgccgg tcagcatgac gatatgagcg ccggcgccat ggcgatgggc ttttttgtac
     1081 ttaccgacag gaaattccga gaggtgagcg ctcatcgtat tatcgacgaa ctcgaaaagc
     1141 gtggtgcggt tgtcgccgcc ccgctccttg tagtctttag gctgaaatcc ccatagatcc
     1201 gcgatgaaat tcgattccca aacgcgtcct cttttcactt cgggaatgta gcggccgcct
     1261 ttgccccatt cgtcggcgcc accgctgtaa cgatcggaaa aagtaaaatt gttattgaag
     1321 atgtagtcga gattgcgata gcggttgacg atcaccggcg cgtccgtcaa ggagatcatt
     1381 cgcaccggtg cattaccttg agcattgaaa tgctggtgcc aggtattgag tggtgtcgaa
     1441 aagagactgc ccttctgcca ctcgagcgtg tgttttttgc ccccttcgtt ccagatcgtc
     1501 gtcgcgcctt ggccttcgag aataaaaatc gccttttcgt acatatggcg tatcggcttg
     1561 agctgaccgc cgggcgcgat ttcctcgatt agtatgtcgc aagtgccgcc cgaaccatcc
     1621 atattgacga agccggcttt gccgccggtg cgagcccagt cgccgagctc caatttgagc
     1681 agatcgggaa tgatgaagtc cttgtagacc ggtatccctt cccattcctg gtaaatctcg
     1741 tagggactct tcggcgaaaa agccgccgct tcattcagat tcaagttctt cgtcattctc
     1801 cggttctcct tctgagtggg atctcgtcct gaggcacttg ccgaacgatc tgctcacaac
     1861 ctatcacttg atctgctcca tgtaataacc gagcgcgccc cggtatgccc ggtcgatggc
     1921 catcgattct cgcgcgccct tcagaaccaa ctcgcaatct tcgggcgtct ttacgtagcg
     1981 ttcgaacatt tccgaaatgt tttccgagtg atcggtatcg gcaaccacgt gcacgtcgag
     2041 ggaaatcatc cgtttcaaat tgatgcccaa ttcgttttcg aattttttgc cgactcggta
     2101 agacatacct ccacccttga cgattttacc attattgcgc cgctcgagca tgtgtgacgc
     2161 ggtgtaagcg gaaagccagt gattatgcat cgcgatatgg tgccaggcaa agaagcaggc
     2221 gcgcaccatc gacgggactt cggctcgttc gaaatcttcc ggcttgagcc cgatcacttc
     2281 gccttgcttg accgatagtg catagtgatc catacccgcg cgcgggtcgt gcaccagctc
     2341 gtcgctctcg tgctcccaga tgatccgttt cacttccagc ggtgcgccac cttgcacata
     2401 accccagcaa tcgcgccggt tgctcgtgta aagcgcattt tgcaccacgt aaaaccggcc
     2461 gcgctcaagc gttaacggca caccgagaaa tttcttgaac tcgacactct cgaattggtc
     2521 gttgaccatt tgatcgagcg ttttcagcaa atcacccaac gtgttcatcg gcgttattcc
     2581 ctccgcttac tgcgcaatct tctacccgtc cggcagagct tcgagcatgt cggcgaggtg
     2641 cccgcgataa acgcgatcga tcatcaggct atcggcggcg ccctgaagaa ttgctttgcg
     2701 cgcgatcacc gtattggcgt atttgcgcgc cacctgcatc aacaaatgcg catgctcgac
     2761 atccatcacg acgtgctccg cggtattaat ttgttttttc atggggatcc cgagatcccg
     2821 ttcgagcata tcgccgatgc gtcgcgacag cgagccgcct tcaatcagtt cgccggaatt
     2881 gcgcatttcg agaatcgctg atgcggcgat cgcttcgagc caggaacgct gcgccaggcg
     2941 gatccatgcg ctgaagcaaa cctcgccacc ttccaaacgc gggttgcgtt cgaacgcctc
     3001 cgcggtcaga ccaaagattt caccttcctt gacggccaaa gtaatgtggt cttgtttgcc
     3061 ttcgcttttc ctgccgatga gctcgtcttc ctcgtgttgc cagatcagtt tcttgatctc
     3121 cagcggcgcc gagccttgca cataccccca acagtcgcgc cggttctgaa catagaacgc
     3181 catctggatc gagtagtgtt gcgcccgcgc tttcgtgaat ttagtgctca gcaaacgcct
     3241 gaacgccttc gattcgaact gttcgttaca catgcgcacg agttccgtcc acatcgcgtc
     3301 aattgcatcc ggcgtcgctt tcatgaaggc gtctctaact tccgatctgg aaaaaagaac
     3361 ttttcgaaat tctcgtcctc aatccgactc tgtcatttcg agcgccagac tgtctctgaa
     3421 tcatggttcg acgagctcac catgagcggt ttagggtttc gtggcgtcaa gtacttagcc
     3481 gtccatcctg agcgtgtcga aggacgacga gcgagttttc gcacagtctg gcggagcgag
     3541 aaatccttct ctcgcttttc ctatctggaa agatttctcc ctgtggtcga aatgacagaa
     3601 atgttcgtat ttgctgcccc ccgccatatc agctgcgaaa acaattcttt tagcatatgc
     3661 aattctgcgc tgtctaacaa tgctcggctg cccttgcaat gtcgcatcat actccgaacg
     3721 ccaggctctt gacgaccacc ggtacgaagc cctcgctttc gacgacttcg ccgatatcga
     3781 tgaagtcgag ctcggacaag cccacctcgt cgatgcatgg catgttccat ttgcccgaaa
     3841 gcataccgcc gacaacctgt ccgatattat ggacgaacac taaggcgacc ggccgatcgt
     3901 cgccggctaa attttccagc gcgcggtcga ttccccgcgc catttcttgc actgcgccgt
     3961 aaccggtgaa tggcggcgtc gaaaacgcga tcacaaacgg ggcgccgcgc acttccgggt
     4021 cacgctcgcc aagcgtccta gcgaccaccg cagcggtctt ttcaagaacc ggtgcttgcc
     4081 aatcgatttc cgcgacaaaa acccgcagat tgcgcgccgg gagggtcatg ctttcaggaa
     4141 taaaaatcgt ttctccgctc aattgtacgg tgtactgaga ggcgccgatc acagtagcac
     4201 gaatgccttc gcccgcagcc aaaatgttga aaccgcgtct ctccgcttca tggcggatcg
     4261 cccggcccag ctcagggccc aaatcgccga aggcgtgagc ttcccgagag taaatgtatt
     4321 cagagacgcc gccggaaaaa agcacgccat cgatggacgg caattcgccc agtgccgctg
     4381 ccgcggtaat gaaaacatcc tccctgggcg cagtcccggt cgtcaatgca tcgaaaagcc
     4441 ctcgcgccat tcgatcagcg aggcgagcct tgagatcctg ggaaatattc tgcccgagat
     4501 ctaaattctg ccccaggtcg agaaggaatt gccggcccgc cctttccagc cggacaattg
     4561 tatttgtcgc atcatgagcg atgaggcgcg cgccgatgtt tagcgcggta gttgcgcgaa
     4621 tcttgccgtt tacgatcaag cttattttgg tcgtgccgcc gccgacatcg atgttcaata
     4681 aggttattcc ctgatcgcgg ctgtgcaaca ccgcgccgga gccatgcgcc gccatgatcg
     4741 tttccaacgt cggcccggcg gtggcgcaca cgaaccgtcc agcttcatcg gaaaatagct
     4801 cggcgatttt ttgcgcattg tcccggcgtg cggcttctcc cgtaataatg acagctcccg
     4861 tgtcgatctc ttctcggctc aaacctgccg cttggaatgt ctggtttacc agctctctca
     4921 acggctccgc ttctatattc caatcgccgc taaaaggggt tagtagaatc ggcgagcgtg
     4981 cgatcacgtt tcgttcgaga acgatgggtt tacgctgaag cactgaggga tagccgatca
     5041 aaagttgcga gaacatgaga tgagatgtcg acgagccgat gtcgataccg acgctcgtaa
     5101 gatgagtgtg cggcccgacg gcgccctcgt gatcgtgtcc gtgctcgcca tgcccgtgat
     5161 gctcgtgggt tacgattgcc atttttagat acgctacttt cgtttcgaat tcgagaactc
     5221 taaaacctta ttcttatttt gtggtcctcg ccaatcattg tcatctcgag caaagcgaga
     5281 gatcttgcgt tcagttattt atccagaaag atttctcgcc cttgggctcg aaatgacaat
     5341 tgagaaggta atcaaacggt gtgaagaagg acgtttccat ttggttcatc aatctgaatg
     5401 aacagatcgt caaccgtaat cttcttcacc aaaagtccct gctcctcggc gtaacgaatt
     5461 aatgtgttca gcactttttc attgccttta acgccaaatt tccacgggtc cggcccgagc
     5521 acgctttgct gctcctccca aacctgacga ttccaagcga aattcgaata ggtggcatca
     5581 gcatcatcgc gcagcgccag atttttcgct cgcaaacaca cctcgaacat cgcctgcgcc
     5641 gccgcgggat acttttccag atacgacgag tgaaacgtga tcgcatgact ggtcgggaaa
     5701 agacccgtgc gccggaaaaa atcctgctcg acggctttcg agtcggcgaa gagccggcgc
     5761 caacctttct cctcgggaaa atcgggcaca atgatcgcat caaattcgcc ttgaagcatg
     5821 cgcgcggcta atgaaccttg tcccggcacg atgtcgatgc gaattcccgc cggcaatgac
     5881 aaatcgagct tggccgggcg cgtgctgatc cagtgaatat cctgcgccct gacaccgtat
     5941 tcgtcgctca gtattccgcg cacccagagt ccacaggtat tgagccagcc cggaatgccg
     6001 acttttttac ccttgagctg cgcaggttcc ttaagcggag agttctccgg cacaaaaatg
     6061 tacgagtggc gaaacttgcg gttcggaaat accggaatcg ccatgtacgg tccgccgcgg
     6121 ctcttgagcg cacaatagtt tgccagggaa aactcgccgg tatcgaactt accggaaaga
     6181 aactcatcat gaccttgacc gtcgttttcg aacggcacgg cgtcgagctc aaagtccttc
     6241 attttgaccc ggccatcaca cagcggccgg gcgcggagat ggtaaccggc ggcaagcgac
     6301 aatcttggct tttggctatt gttcatcgca aactcctcag aatggaatca tcgacgattc
     6361 atcgatgggc agcgagcatt tgattgcgcg gatggtcgag tgaggcttta actctgcgag
     6421 catggaagtt ctttctcatt tcaatccgcg gagcagggcg aaatcgaaca tttcggcggt
     6481 actcaggcgt ttgggaatct cgccgcggcc aaacgaaccg tcgagatatt tttgaatttg
     6541 ttcgtcgcgt aaaagcagcg gctccaccat gactcccata atgtcgtcgt aggattcgtt
     6601 ggcattggct gcgctgactt tgaaattctt ctcgatgtac gtcaccaccg cggatctctc
     6661 ccgcttgatc gcagcaatga catcgcgcat ggaacgcaac atgttcttca cctgttcggc
     6721 ttggcgctcg atcttttgac ggctggcaac taaacccagc cacggcgcat cctgaagcag
     6781 cgaacgactg cgcgccagga tcttgtaacc cttttccgcc atgattccgg tgaagggcgg
     6841 gctcagcacg gcggcgttca ccgaattctg ttggagcgcc gtcaagctca ccgtggtgct
     6901 gccggtctgt ataaatgtta ctttttttgg atcggcacca ccgctttcga tcatcaatga
     6961 gccgtatcgg tggctttccg aaccgatgcg cgataccgcc acggttttgc cttccagatc
     7021 cttcacgctc gcgagcccgg cgggactgac cagagaaaat aatggtgcgc gataaagcac
     7081 cgccagagct tttatcggca atccattcgc gatcgccgca ccggccggac cgaccgatgg
     7141 agtgaagtcg atctcgcccg acgccaaccc ggcgagctgc aaatcactgc gcatctgaat
     7201 caactccacg ttcaagccat gcttttcaaa aattttgatg tccttcgcgt agtagaatgg
     7261 caggaaaaca acgttacgca cggacaaagc catccggaca cgctcctgcg cagccgcatc
     7321 gttgacaggc atcgtggcag caacaaccgc actcaaaaga aaaagaactc cgcctttgaa
     7381 aaagcgggaa ttgaaggggg atttgcgagc gagcaactcg gaaaatctcc cgttaattcc
     7441 ctttttcaaa gaggggaaaa tcaagttcca tacggagttt gggcgcgaca tagataaacg
     7501 gagatttgat tgatatccgc tggcgacatt acgcaatttt cgggtcaatt caacatactt
     7561 tctcaggcct taatccctct ctcggcttct tccatcgccg cccgcaacat cacgccatgt
     7621 aaatccactg aggtcatggc acagtactcg atcccgtaag ttggccgttc atcggccata
     7681 ccggtttcga gcaaacgctg caacgccata cgattgaagc tcgcatggcc catgactcct
     7741 tcctcgtgtt cggccaaatc agcttccaca tgggtagcga agtaagtcgc tttgtctcga
     7801 tcaaagccat agtgcgtcgt tagcgcttga tagcaggccg ccgaccagtg gccgatttgc
     7861 tcctcggtgg tgaccgcccc ataccactcc gccgccgtgc cctcgtaaac gatgccgcgc
     7921 atgaaatcga ttttgccgcg aaactccggg agcatcggcg tggtgaacac ttcttcctcg
     7981 gtcaaaccga aggcttgagc ggtctggagc atcacaagaa aatggcccgg cggtttggga
     8041 tgaatgaatt catcggcaat tttctcgccg atcggcccca tcaaatcgcg attcactttc
     8101 aggaaggtaa tgttccggtg gtacgtgcac gcggtgagcg tattgatttc gatcgtgtac
     8161 gcgccccaat ttcgaaaaaa tgtttgcaac actttgagcg gcagctttcc ggccatcagt
     8221 tgctgcatga atggcttttg gttgacatta gcttcccaac gttcgtgcac gagcgaataa
     8281 agcttatcga ccgttttctt cgcctcggcg accgagtatt tttttccccg ctcaattttg
     8341 agtgacaaaa ctcacctccg gctcgaatag tgttccgcgc gccccggctc tacttaattt
     8401 tcagctcctt ctgcgcctcc cgcagcagcg tgaaatcccg gacttcatca acgggtttct
     8461 tcttttccgt cagagcatcc cattcctgat cagtgatctc gccattccgg ctgagcgaac
     8521 ccaccaagag atcgtaagag cgttcggcaa cgtcgggcgt ctgatccaaa tagcggatca
     8581 tttgcggcgg aacctctttt ctgttctcga agacgaaacg atgcgctttc aacagcgcgc
     8641 ggacggatcg ctttacaagc tgcggattct gctgcaatgt ccgattggac actgccaagc
     8701 ccgaagtggg tagcgcgatc tgcacatcct gcggacccgc caggaagcgg aacccttcag
     8761 atttcaggct gagatcgaat ggcgaggtga tcgagatggc atccaccagc ccggcgcgca
     8821 acgcctgcgc tctgaccggc gattctccca aggcgatggg cttcatcatc gtcggggaaa
     8881 atcccttgcc ctttaacatc tcttccgcgg cccgttgatc ggagccgaac aaggtcgcta
     8941 cgccgagttt tttgccgttg agatcacgaa tttctttgat ttcggggcgt accactaaaa
     9001 tgtgctcgga cttttttacc gcgacagcga caaatttgat tggaaagccg ccgataatac
     9061 tgcgcagtgt ggtaccgaac gtcgtagtca aatcgaggtc tccattgatc acggccgtcg
     9121 cggccagacg tgggttgacc tgaatcagct ccacgtccat tccttcctcg acaaaaaacc
     9181 ctttattcag agcaataatg aatggcgtgt tcgaattgct ccggctcgaa tacgaaattc
     9241 gcaccttggt caattgcgcc gtgatcccgt cacccgcgac aaaaagaatg ctgaataggg
     9301 caatcgtgat cactcgagga gggcgcaacc tttccagcgc ttcataccga tcgctccaat
     9361 ctttagacga accggtccgg agagaacggc gtggactttt tgtccgcacc gccaagtaaa
     9421 agttcggccg ccaactcacc aaccgccgaa ctagtcgtca tgccatgacc gcccagaccg
     9481 gcaacccaga agaaatgttt cacgtccgga tcccagccga taatgaaccg gccatccggg
     9541 gtcaaggttc taaacccggc ccaacctctc gctatactga ccgaagaaag ccctgggata
     9601 aattccccga tcttctccgc gagtagttct tgcacagagc cgtcaacggg cggatcgcct
     9661 ggagccaact cagtttggtc gcacgcgcac agcagcagac cttcgccttc cgggcgaaaa
     9721 taaatatcat gcgtgacatc ccaaacaaac ggccaactcg gatccaccca accaagcggc
     9781 ggtgaaacaa atagatggcg ccggcaggga tgcaacggca tttctcgtgc gcctaccatt
     9841 ttggcaatga caccggccca ggcgccggcg gcgttgatta cggtgcccgc accgatagtc
     9901 tcgccttcaa ccttgatgga aatctcattt gagctattac gcagctcgcg aactttgcag
     9961 gcgtaacgaa tgcgcgcgcc gttccggacc gccgccttta aataacccga aagcaacgca
    10021 tgaatatcga tgattccatc cgttgcgcac cacgcggcgc cttcgaactc cgcgccctgt
    10081 aaaatcggca cgcagcgctt cgcttgttcc ggcgtccaca actctgtttc tatccccacg
    10141 ctccggccga ttgcggcatc atgctgaagt ttctgccaac cggcgccgct gcccaaaagc
    10201 aaagaaccgt tctgcttaaa ttccaccggt tccggccagt cactcgggaa ctggcgcaaa
    10261 aacgcgccgc ctgcgcgggt gagcgccgcc aggtcgggat caggaacaca ttgccgcatc
    10321 atcgcggcat tgcggccgga cgaatgaaaa ccgggaatgc tttcctgctc gagcaagagc
    10381 acactgcgcg cgccacggcg ggtcaggtga taagcggtcg cggcgccggc aaaaccggcg
    10441 ccgataatga cgtaatcgta atattccata accttccaat gtaatcacta ctatatgaac
    10501 tgagaacgaa tttccaaagc tccgcagagc ctgattgtcg cgcgccgact ggcaaattag
    10561 ggtttgaaag ccggttcaac tgcgctatag tcaacttcaa atgagaatcc atcgctacac
    10621 tgagtgggat ggcaagcaac aggttctctt ccccaccacc gacgatttgc tcaagcatct
    10681 ctccgatcac ttgctcgaag aagaaggtgt gcgccgcgcg ctgcgcgatc ttatgcgccg
    10741 gggatttacc tcggaagatg ggcagcgctc cgtcaaggga atgcgggatt ttttgcgaga
    10801 agccgacgag aagcgccgcg agttgctcaa caagtattct cccgattcct tcaagcttac
    10861 cgccgaagag cagaaaacgc tgagtgaaaa gctcaacagt ctggcggaaa agctcgaaaa
    10921 gtatcatgag cagatgcgca attttatgga gcgaatgtcg ggcaagttcg ccgaccgcat
    10981 ggatgagctc agcgagaaaa tgcaggaggc ctaccagcgc taccaagagc tgagacagcg
    11041 gctgcaaaat cagatcgccg agcgcggcat gcagaaacag tccaacatga gcggacagga
    11101 cccgcagtct ctcatggaca tgttggaccg catgcaaaag ctcctggaag atgaagattt
    11161 tctcaaaaac cttcctagca tgatggacgc cgcagttaat aatctggaaa gcatgctgga
    11221 gaatctcgac tccatgactc aggagcagct gcagcaattg agcgacatga tgactcaaat
    11281 gcagcagctc gagcagctca tgaatcagtg tggttttcag ggctcgcagc gcatggggat
    11341 gggcgaagcg ggtcaaattc tcggtcagct ccgcggtctg gagcgttttc ttcgatgggg
    11401 acaacgcggc atgggcgatc cctcggagct gaacctcgat gagctgcgtg aaattcttgg
    11461 cgaggaagcc tatgagaatc tcaagtattt aaaaggcgtc gagcaggcgc tcgaggatga
    11521 gggttacctc gttcgcacca accatggcct gcgcttgaca cccaaaggca tgcgcaaaat
    11581 cggcgacaaa gcgctgcgcg aaatctttca aatgctcaac aagagccgct ggggcaatca
    11641 cgccacatcg tcgcgcggct ctcagggcga tcggatggag gagaccaagg aatacgaata
    11701 cggcgatccg ctccacgtga acatcggcga gacgctgctc aacgcgttgg agagacgcaa
    11761 gaggggcgaa ccgctacgca tcgcgcccga agacttttcc gtgcaccggg tcgagtactc
    11821 gaccgtcagc gagaccgcgc tgttgatcga tgtaagctac tcgatgttga tgaacgatgc
    11881 cctgcacgcc ggcaaaaagg ttgcgctcgc gttgcaccgt ttgatccaga cgaaatatcc
    11941 tcaggata
//
LOCUS       DASULZ010000630         2568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_274624, whole genome shotgun sequence.
ACCESSION   DASULZ010000630 DASULZ010000000
VERSION     DASULZ010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274624"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /gene="trpB"
                     /locus_tag="VFY96_13025"
     CDS             complement(<1..981)
                     /gene="trpB"
                     /locus_tag="VFY96_13025"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX5607438.1"
                     /translation="MRNSKNLSLLPRVPDRRGHFGPYGGRYVAETLMPALAELEAAYA
                     KARRDPSFRREFHLYLREYVGRETPLYFAERLTQQIGGAKIYLKREDLCHTGAHKINN
                     TIGQILLARRMGKQRIIAETGAGQHGVATATVAARFGLTCEIFMGKEDVRRQALNVFR
                     MKLLGAKVRVVEAGSQTLKDAMNEALRDWVTNVRSTYYLIGSVAGPHPYPMMVRDFQS
                     VIGAEARRQILKKEGRLPEYLVACVGGGSNSMGLFYPFVRDRGVKMLGVEAAGHGIPT
                     GKHSASIQGGAVGILHGSKSYVLQNDRGQITETHSIAAGLDYPGVGPELSY"
     gene            complement(938..1582)
                     /locus_tag="VFY96_13030"
     CDS             complement(938..1582)
                     /locus_tag="VFY96_13030"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX5607439.1"
                     /translation="MLPAFMNVKVKVCGITSLADAEKSLELGADMLGFNFYPPSPRFL
                     TPQKAREIIQRLPADSFNVALFVNEPRESVSDVIARDQLDNGRSRYLAAQFHGEENAK
                     YCFGWEIKVIKAFRLKERSSLMALSHFPADFYLLDSWSPGYGGSGAAFPWEWADGLEA
                     SKLILSGGLCVENVAAAIKRVHPFAVDVCSGVEARPGVKDYAKLKEFIIAAKSA"
     gene            complement(1588..2367)
                     /gene="trpC"
                     /locus_tag="VFY96_13035"
     CDS             complement(1588..2367)
                     /gene="trpC"
                     /locus_tag="VFY96_13035"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX5607440.1"
                     /translation="MAEILATIAEHVRGVIERQRSKVPIAALRDRPLFKAATRGFARS
                     LTGDGRYIIAEIKRASPSKGLIRADFDPVWIAKNYAAHGAAAISVLTEERFFQGRLQD
                     LEQARAAVDLPLLRKDFILDPYQIVEAKSYGADAVLLIAALLEPGLIRELRELAETLS
                     LDVLVEVHTRNELEAAVIAGAHVIGINNRDLTTFEVSLSTTEQLASLVPPNALVVCES
                     GIDSFDAIRRIEKVGVHVFLIGESLMRAPDPGARLSELLLG"
BASE COUNT          560 a          790 c          673 g          545 t
ORIGIN      
        1 atagctcaac tccggaccta caccagggta gtctaaaccc gccgcgattg agtgagtctc
       61 ggtaatttgc ccacgatcat tttgtagtac gtagctcttg ctgccgtgca aaattcccac
      121 cgcgccaccc tgaatcgacg cggagtgctt tcccgtcgga attccgtgtc cagccgcttc
      181 gacaccgagc attttgacgc cgcgatcgcg tacgaacgga tagaatagac ccatagaatt
      241 acttccgccg ccgacgcaag ccactagata ctccggcaat cgaccctctt tttttaaaat
      301 ctgccggcgc gcttccgcac cgataaccga ttgaaagtcg cgcaccatca tcggataggg
      361 atgcggcccg gccaccgacc cgatcaagta gtacgtactc cgcacattgg tcacccaatc
      421 ccgcagtgcc tcgttcattg catctttaag tgtttggctt cctgcctcca ccacccgcac
      481 cttggcaccg agcagcttca tgcgaaacac attcagcgcc tggcgccgga catcttcctt
      541 gcccatgaaa atctcacagg taagaccaaa ccgggcggcc acggtagccg ttgctacgcc
      601 atgttgccca gcgccggtct cggcaataat gcgctgctta cccatgcgcc gcgcgagtaa
      661 aatctgaccg atcgtattgt tgatcttgtg cgcgccggtg tgacacaagt cttcgcgctt
      721 gaggtaaatc ttcgccccgc cgatctgctg cgtcagtcgc tcagcaaaat aaagcggcgt
      781 ttctcgcccg acatattcgc gcagatataa atgaaactcc ctgcggaacg agggatcccg
      841 gcgcgctttg gcgtaagctg cttcgagctc cgccagggcg ggcattaacg tctcagcgac
      901 ataacgtccg ccgtacggcc cgaagtggcc gcgccggtca ggcactcttg gcagcaatga
      961 taaattcttt gagtttcgca taatccttga cgccgggccg cgcttccacg ccgctacaga
     1021 catcgactgc gaaagggtga acgcgcttga tcgccgcggc aacattctcg acacaaagtc
     1081 caccggagag aataagcttg ctggcctcaa ggccgtcagc ccactcccac gggaatgcag
     1141 cgccggaacc gccgtaacca ggagaccacg agtcaagcag ataaaaatcg gcgggaaaat
     1201 ggctgagcgc cataagtgac gacctttcct tcaggcgaaa ggctttgatg actttgatct
     1261 cccagccgaa gcaatatttc gcgttctctt caccatgaaa ttgcgccgcg agatatcttg
     1321 accgtccgtt gtcgagctgg tcgcgcgcaa tcacatcgga gacgctttct ctcggttcgt
     1381 tcacgaacaa cgccacattg aacgagtccg ccggcaaccg ttggatgatt tctctggcct
     1441 tttgcggcgt gagaaaacgc gggctcggcg gataaaaatt gaatcccagc atgtccgcgc
     1501 cgagctcaag cgatttttcc gcgtcagcca agctcgtaat accgcaaact ttgactttga
     1561 cgttcataaa agcgggtaac acgcaaatca tccgaggagt agttcagaaa gtctggcgcc
     1621 tggatctgga gcgcgcatca aagactcgcc gatcaagaat acatgcacgc ctaccttctc
     1681 gatcctgcga attgcatcga aactgtcgat gccgctctcg cacacaacca gggcgttcgg
     1741 aggaaccagc gaggccagct gctccgtggt cgaaaggctc acctcgaacg tagtgagatc
     1801 gcggttattg ataccgatca cgtgcgcgcc tgctatgact gcggcctcga gctcgtttct
     1861 ggtatggact tcgacgagca cgtcgagcga cagcgtctcc gccagctcgc gcaattcgcg
     1921 aatcaaccct ggttcgagca gcgccgcgat cagcagcacg gcatcagcgc cgtaactctt
     1981 cgcctcgacg atctgatagg gatcgagaat aaaatctttg cgtagtagcg gcaagtcgac
     2041 cgccgcccgc gcctgttcga gatcttgcag cctgccctgg aaaaatcgct cttcggtcag
     2101 caccgaaatc gccgccgcgc cgtgagccgc ataatttttc gcgatccata cagggtcgaa
     2161 gtcggcgcga atcaagcctt tggacggtga agcccttttg atttcggcga taatgtaccg
     2221 cccatccccg gtcaggctcc gtgcgaaccc gcgggttgcg gctttgaaaa gcggccgatc
     2281 ccgcaacgca gcgatcggca ctttactccg ttggcgttca atcacacctc ggacatgctc
     2341 agcaatggtg gccagaatct cagccatggt gaggtccgga ttcgctctga atagagcgat
     2401 tgcgtcgtcg ttggagcgcg gcgcggcgca tcgctgtccg cccactaagc cgcattggtg
     2461 agctcaatca gctgggcaag ttttttgcgt gcgttccccg aatcgatcga ctctgcggcg
     2521 agcttcatcc catctgggat cgttgcggca tttccgctga tatacaaa
//
LOCUS       DASULZ010000631         2126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_375350, whole genome shotgun sequence.
ACCESSION   DASULZ010000631 DASULZ010000000
VERSION     DASULZ010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375350"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..1791
                     /gene="recJ"
                     /locus_tag="VFY96_13040"
     CDS             31..1791
                     /gene="recJ"
                     /locus_tag="VFY96_13040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX5607441.1"
                     /translation="MPPASRKRWLLRDADPQAVAEQSEQLKVSPLLARILILRGYSDG
                     QSGKRYLSSSLREDLPSPFHMIDMEPAVARLVRAIEDKEQIGVWGDYDVDGTTGASLL
                     VCFLRAVGAQPIYYVPHRIEEGYGLNTAGLRRLKAQGVELVVTVDCGISNAIEVEAAR
                     EFGLEIIVIDHHQPPSELPRALAVVNPHRKDCPFPDKGLCAVGLAFYVVIALRAKLRE
                     SGWFANGALDIRPYLDIVTLGTIADMVPLRGVNRTLLRRGLQELTSSRRPGIVALKKV
                     AGIPDGDVSAGQVGFRLGPRINAAGRVDYGIKVVELLTTESSEIAARIAQELEAHNSE
                     RRAVEAVVLEQALARAATVMDGGSCHSLVLAGAGWHAGVLGIVASRIVEKYCRPTVVI
                     GFEGDQGKGSARSIRGFHLVQGFQHCAEHLEKFGGHEYAGGLSIKAAKLERFADEFEK
                     FARQALMPEDLLPLVEIDAELQFSQIDIGLLRELNVLKPFGVGNPEPLFMTSGAEICE
                     RRTFSSGVRYRLRQAGRAISSVMFGVPDELPGAPGELIDIAYRLTRNEWNGTWTPELR
                     LADLRMPESLSASFENRVLR"
     gene            1829..>2126
                     /locus_tag="VFY96_13045"
     CDS             1829..>2126
                     /locus_tag="VFY96_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607442.1"
                     /translation="MTPWRVVFFGIAWILTTAPVFAADLGGKPEKSDVVVSYAQPSGA
                     FTPIWVAYEAGLFKKHGLNANLQLLTPQVSAQAVISEEVDFYTDGPDLINARLRG"
BASE COUNT          430 a          562 c          652 g          482 t
ORIGIN      
        1 agctccagcc cgttgacagc cgtagagtaa atgccgcccg cttcgcgcaa acgctggctt
       61 ctcagagacg ccgatccaca ggccgtcgct gaacaatctg aacagctcaa agtctctccg
      121 ctgctcgcgc gcattctgat tttgcgcggc tattcggatg gccaatcggg caagcgttac
      181 ctgtcatcga gcctgcgtga ggatttgccg tcgcccttcc atatgatcga tatggaaccc
      241 gcggttgcgc gactcgtcag ggccatcgag gacaaagagc aaatcggcgt atggggggat
      301 tatgacgtcg acggcaccac cggcgcttct ttgctggtat gctttctgcg cgccgtcggc
      361 gctcagccga tctattacgt gccgcatcgc attgaagagg ggtatggtct caatacggcc
      421 gggttgcggc gcttgaaagc gcagggtgtc gagctcgtcg tgacggtgga ttgtgggatt
      481 tccaacgcca tagaagtcga agctgctcgc gaattcggcc tcgaaatcat tgtcattgat
      541 catcaccagc cgccgtctga actgccacgg gctttggccg tggtcaatcc gcaccgtaag
      601 gattgtcctt ttcccgacaa agggctttgt gcggtgggat tggcgttcta cgtcgtgatc
      661 gcgttgcgtg ccaagttgcg cgagagcggt tggtttgcga atggcgccct cgatattcga
      721 ccttatctcg atattgtgac gctcggaacg atcgcggaca tggtgccgct caggggcgtg
      781 aatcgaacgc tgctgcggcg cgggctccag gagttgacct cttcgcgccg cccgggaatc
      841 gtcgcgctaa aaaaagtcgc aggcattccg gacggcgatg tgagcgctgg acaggtcggc
      901 tttcgtttag gccctcggat caatgctgcg ggccgggtag attatggcat caaagtcgtc
      961 gaactattga cgaccgaatc tagcgaaatc gcagcgcgca ttgctcagga actcgaggct
     1021 cataatagcg agcgccgcgc ggtggaagcg gttgtgcttg aacaagcgct cgcccgagcc
     1081 gctaccgtaa tggacggcgg cagttgtcat tcgctggtgc ttgccggcgc cggctggcat
     1141 gcgggtgtgc tcggcattgt tgcttcgcgc atcgtggaaa agtactgccg gccgacggtc
     1201 gtgattggtt ttgaaggcga tcagggcaaa ggatcggcgc ggagcatccg cggctttcac
     1261 ctggtgcagg ggtttcaaca ttgcgcagaa catttggaga aattcggcgg gcacgagtat
     1321 gccggcggac tgtcgatcaa ggctgcgaag ctggagcgct tcgccgatga gttcgagaaa
     1381 ttcgcccggc aagcgctcat gcctgaagat ttgctgccgc ttgtggaaat cgatgcagag
     1441 ctacaatttt cccaaattga tatcggactc ttgcgggagt tgaacgtgct caagccgttc
     1501 ggggtcggca atcctgagcc gttgttcatg acaagtgggg cagaaatctg cgagcgaaga
     1561 acgttttcaa gtggcgttcg ttatcgtctc cgacaagccg gtcgcgccat cagcagcgtt
     1621 atgtttggtg taccggacga gcttcccggc gcgcccggtg aattgatcga tatcgcatat
     1681 cgcctcactc ggaacgaatg gaatggcaca tggacgccgg agttgagact cgccgatcta
     1741 aggatgccag aaagcctctc ggcgagtttc gaaaacagag tcttacggta aatctattta
     1801 tttctaacct gagcctggag gaaaaggcat gacgccctgg agagtggttt tttttggcat
     1861 tgcatggata ctcacgacgg ctccggtctt tgccgccgat ctcggcggca aaccggaaaa
     1921 atcagatgtt gtcgtgagct atgcgcagcc gagcggcgcg ttcacgccca tttgggtcgc
     1981 gtacgaagct ggtctgttca aaaagcacgg tttaaacgca aacctacaac tcctcacacc
     2041 acaagtttct gctcaagcgg tgatttcgga ggaagtggat ttttataccg acggtcccga
     2101 tctaatcaat gcgcgcttgc gtggcg
//
LOCUS       DASULZ010000632         2896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_579918, whole genome shotgun sequence.
ACCESSION   DASULZ010000632 DASULZ010000000
VERSION     DASULZ010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579918"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1174
                     /locus_tag="VFY96_13050"
     CDS             167..1174
                     /locus_tag="VFY96_13050"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607443.1"
                     /translation="MKTDSFVKCASLFCFGLAIFSGHDVQLFAADKLNLIYTARVMSQ
                     SYPWMAQDLGLFKKYDLDVPAVFVTPGAPSVAAILSGDSEVAVIGAASITRPFVQGNK
                     DPVLIGGIKSLLTHSIIAKPEITRPEQLKGKRIGVSRIGSNPHYFAVQALRHFGIDSR
                     EVSYIQAGGAPETLAALVAQGIDAAVLTVPTDAQALKLGYHYVIYGPDLKIAYAATTI
                     TTRRSIIAKRGPVMGRFMRAMAEAAKIMHTDKDLTFKILGKYLRIDDRALLDASYNVE
                     IKALEPRLALKLDGIQSTLDEIAPTEPRAKTVKPQEMIDTRYLDEMEKGGFFDQMWGR
                     K"
     gene            complement(1280..2227)
                     /locus_tag="VFY96_13055"
     CDS             complement(1280..2227)
                     /locus_tag="VFY96_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44 domain-containing protein"
                     /protein_id="HEX5607444.1"
                     /translation="MLISGRKIVIVALGLFAIFGLSQIILEQCADARAGGGRSGGFRG
                     SRSYQAPARPAQPTSPRREASPTPQQPAPYTPAGGGFMRGLGTAILGGFLGSMLFSGI
                     ANAGFGGFGGSGFGLIEILLLAGLGYFLLQKFRRPAGATGYQPMQYQATPEFPPRTAS
                     LEDAPSTNRGIDYRSLTMMDRTFDPDRFLKTAQDLFFKIQGAWNKQDTTTLSSLCGSE
                     LMQSWQEELTKQRALGQQNRMENIALRESEISEAWTENGADFITVHLHANLVDYTVDA
                     RTGNVVSGSKTEPIEFEEYWTFSRPVGPNSWKLSAVQQA"
     gene            2397..>2896
                     /locus_tag="VFY96_13060"
     CDS             2397..>2896
                     /locus_tag="VFY96_13060"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607445.1"
                     /translation="MPLMCLLVVAFFSSVLLPFAPRLARAQTKITVGVAAMSPRTIPL
                     LIAQEQGLFAKHGIEARIVLIKGAPTLVASLISGDIEIGYTGGTAVLGAASQGLYLRI
                     LSSISSKLTHSVVANPSLKTPEQLRGKRFGIQSIGGSTWMHTILGLEHIGLDVKRDNI
                     NLLVIG"
BASE COUNT          648 a          758 c          841 g          649 t
ORIGIN      
        1 gctatagaca cgatgagttt gtcaaaggcc tcgggcccgc agcaggaaaa atagccgata
       61 gccgataact tcgaatgccg acatatggct acgtcgttgc tctttaagga cgtggtgttg
      121 taataacggc ttgctcagga atgtttccaa attcagggag tccggtatga agacagacag
      181 tttcgttaaa tgtgcgtcgc tattttgctt tgggctggcg attttttcag gccatgacgt
      241 acagctattt gcggcggaca agctcaattt gatttacacc gcgcgagtca tgtcgcagtc
      301 ctatccgtgg atggcgcagg atctggggct gttcaagaaa tatgatttgg atgtgccggc
      361 ggtttttgtt acgccagggg cgccctccgt ggcggcgatt ctttccggcg acagcgaagt
      421 cgcggtgatc ggggcagcga gcattacgcg gccttttgtg caagggaaca aagatccggt
      481 attgatcggc ggtatcaaaa gcttgctgac acacagtatc attgcaaaac cggagatcac
      541 gcggccggag caactcaaag gaaaacgcat cggcgtctcg cgcatcggca gcaatccgca
      601 ttatttcgca gtccaggcgc tgcgccattt cgggatcgat tcacgcgaag tgagttacat
      661 tcaggcgggt ggcgctccgg aaaccctggc ggcattggtg gcccaaggta tcgacgctgc
      721 ggttttgacg gttccgacgg acgcgcaagc gctcaagtta ggctatcatt acgtgatcta
      781 cggcccggat ttgaaaatcg cctatgcggc gacaaccatc actacgcggc gatccattat
      841 cgccaaacgc ggcccggtga tgggccgctt catgcgcgct atggccgaag ctgccaagat
      901 catgcatacg gacaaggatt taactttcaa aattctcggg aaatatcttc gcattgacga
      961 tcgggctttg ctcgatgcaa gttataacgt tgagatcaaa gcgctggagc cgcgactcgc
     1021 gctgaagctc gacggtattc agtcgactct cgatgaaatt gcgcctacgg agccgcgagc
     1081 aaaaacggta aagccccagg agatgatcga taccagatat cttgacgaga tggagaaggg
     1141 tggatttttc gatcagatgt ggggacggaa gtagcgtgaa gcgtcgggcg tccggttgaa
     1201 gggtgagcga tggcagcagc acaaaaaaca acggcagcgc gggttagatg gcgctgccgt
     1261 tgtcctgcgc agtcgatcat tacgcctgct gtaccgcgga tagcttccaa ctgttcggcc
     1321 cgacggggcg gctgaacgtc cagtactctt caaactcgat cggttcggtc ttgctgccgc
     1381 tcacaacatt gccggttcgc gcatcgacgg tgtaatcgac caaattcgcg tgtaagtgca
     1441 cggtgatgaa gtcggcgccg ttttccgtcc aagcttcgct gatctcactc tcacgcagag
     1501 cgatattctc catccggttt tgctgcccga gcgcgcgctg tttggttagt tcttcctgcc
     1561 agctttgcat taactcggag ccgcagagtg agctgagcgt tgtcgtatct tgcttgttcc
     1621 atgcgccttg aattttgaaa aagagatcct gcgcggtttt gaggaaccgg tccggatcaa
     1681 aagttcggtc catcatggtc aaagagcgat aatcgattcc tcggttggtc gatggcgcat
     1741 cttccaatga cgccgtgcgc ggcgggaatt ccggtgtggc ttgatactgc atgggttggt
     1801 agccggttgc tcctgcggga cggcgaaatt tctgcagcag gaaatagccc agccccgcca
     1861 gtaataaaat ctcgattagg ccaaagccgc ttccgccgaa accgccgaag ccggcgttgg
     1921 caatcccgga aaaaagcatc gaacccagaa atccgcccaa gatcgctgtc ccgagcccgc
     1981 gcatgaaacc accgcctgcc ggtgtatagg gagcaggttg ttgtggcgtt ggagacgctt
     2041 cccgtcgcgg tgatgttggc tgagccggac gagccggcgc ttgataactt ctcgaaccac
     2101 gaaaaccgcc cgagcgacca ccgccggcgc gtgcgtcggc gcactgctcc aggattattt
     2161 gcgaaagccc aaagatcgca aaaagaccga gcgcaacaat gacaatcttt cgtccggaga
     2221 tcagcataga gtgccctcct gatgagcgca gtgtaagctc aagaaagcta atgtcaatat
     2281 agcggaaaat ggttgaccgc ttgcggccga ttctcgtatg taatgaaagc cgaacggggc
     2341 gattagcccg gggccagaga ttcgaatgct tgcggctcca ggtgaggagg aaagccatgc
     2401 ctcttatgtg tctgttggtc gttgcatttt tcagctcggt cttattgcct tttgctccta
     2461 gactcgcgcg cgcccagacc aagattactg tcggggttgc ggccatgagc ccacggacga
     2521 ttccgcttct aattgcgcaa gagcaagggt tatttgcgaa gcacggcatc gaagcgcgta
     2581 ttgtgctgat caaaggcgcg ccaacattgg tggcgagtct catctccgga gatatcgaaa
     2641 ttggctatac cggcggcacc gcggttttgg gagcagcatc gcaagggctc tatttgcgaa
     2701 ttctctcatc gatctcaagc aagctcacgc attcggtcgt tgccaacccg agccttaaga
     2761 ccccggagca gttgcgcggc aagcgcttcg gcattcagag catcggcggc agcacctgga
     2821 tgcacacgat tctcggactc gaacacatag gtttggatgt caagcgcgac aatatcaacc
     2881 tgctcgtaat cggcga
//
LOCUS       DASULZ010000633         4002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_375568, whole genome shotgun sequence.
ACCESSION   DASULZ010000633 DASULZ010000000
VERSION     DASULZ010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375568"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFY96_13065"
     CDS             <1..204
                     /locus_tag="VFY96_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607446.1"
                     /translation="KVNRPTWFLCLPFSQVPQEPVMCPSSAHPPLYHPLVPQVPQARP
                     ELALAEAAAELVLLSYRSPQTSK"
     gene            complement(1191..1802)
                     /gene="lptE"
                     /locus_tag="VFY96_13070"
     CDS             complement(1191..1802)
                     /gene="lptE"
                     /locus_tag="VFY96_13070"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEX5607447.1"
                     /translation="MQKLHKVLRWSGGWLGVLTVSLASGCGYQFVGESSLLPKDARTI
                     YVEPFINRSRDVGIERELTTATRGEFYRHGELRVVDSSDQADVILSGVIRSLDSTVAT
                     VNREDEVLQYDAVMMLDITVRRREPDEILWRGQGVRFNQVYGGSRAAVVTTSSEFRTG
                     TLNVSDVRRMTDIQLTESEHRAVRAQLMEQFARELRQRIFEMF"
     gene            complement(1805..>4002)
                     /gene="leuS"
                     /locus_tag="VFY96_13075"
     CDS             complement(1805..>4002)
                     /gene="leuS"
                     /locus_tag="VFY96_13075"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX5607448.1"
                     /translation="QWNQWIFLHFFKKGLAYKKNSFVNWCPSCETVLANEQVIDGRCW
                     RCDSAVTQKELEQWFFRITEYAEELLRDLEQLPGWPDKVLIMQRNWIGKSVGAEIDFP
                     LVGREGALRIFTTRQDTIFGATFVSLAVEHPMALTLAAGTQHESAVRNFIDRFKNVSQ
                     TNRGAEEGEKEGVFTGAYCRNPFTQENIPIYLANFVLMEYGTGAVMAVPAHDQRDFEF
                     AKKYRLPIRVVIQPVNQKLKAESLSEAYVDDGVMIDSGDFSWLTNTAGKEQIAARAEA
                     NGWGKKAIRYRLRDWGVSRQRYWGTPIPIIYCDRCGTVPVPEEQLPVRLPEDVPFTGK
                     GGSPLRESKSFVEVACPKCRGRAWRETDTMDTFVDSSWYFLRYASPHYADAPFDKGQG
                     DYWMAVDQYIGGVEHAVLHLLYARFFTKALRDLGLTRTGEPFTNLLTQGMVSKETYRC
                     PEHGWLFPGEPAGSEKDGWNCPQCGRPVEKGRVEKMSKSKKNIIDPEDLVNLYGADTA
                     RLFTLFAAPPEKDLEWSDQGVEGAYRFLSRLWRFVYQHRTIWSSIASTSEPGELTAEL
                     RDLRRMTHRTIKKVSDDIDGRFHFNTAIAAIMELFNAVSALASEFGSESAKPVVKEGV
                     ETIIALLTPFVPHVAHELWQELGHKDGSLETAWPAYSEDALAEEEFLIVVQVNGKIRG
                     KITVPAEVTQEYIEKSALADPKVTGFIDGRTVRRVVYVPRRLVNIVVEG"
BASE COUNT          910 a         1169 c          986 g          937 t
ORIGIN      
        1 aaggtgaatc gccctacttg gtttttgtgt cttccttttt ctcaggtgcc acaggagccg
       61 gtgatgtgtc cttcatcggc tcacccgcct ttgtatcacc cgctggtgcc gcaggttccg
      121 caggcgcggc cggagctggc gctggcggag gcggcggcgg agctggttct tctttcttac
      181 cgcagcccac aaacgtcaaa gtaaaagcga agagggtgac cgctgacaac gatacgagtt
      241 tcttacccat ccttagttca cctcctttcc cgtcacactt aaatctttgt ctgttaagca
      301 ttttgtatcg cacatgtgga tttaagattc aagcacagaa acggcctaat cgcctcagtt
      361 ttctgcttga ttacagcgcc gcggcttacc ggctggggat tttcgctccg caccgcagca
      421 caggttcatt acgtactgat ttagagtgct cggctgatga ctctcacggc aagcgcacac
      481 gcggagcaca tggttttaac gcgttccaca gccgatttgt ttagaatgcg gtcgtcacag
      541 tgaaacatcc gcgacaattt gacaccaaat gttaataaat tgttgataac ccggcacgag
      601 catgccgcac gaggctagaa atcgcggacc ggagcgtttt gcctaattta caagcgggtc
      661 aaagctcgcc gtttcaagga cttaaaaatt tgcttgcgca aggacttcaa tccgaaaaac
      721 gagcctaggg cgcccaacct ccatagagct ttgaacggcg atacgagcgc gagcgcggaa
      781 atcagaatgt tttgtcaatc agactgcgct gcttcgttga cacatccgtt acgcgatgtt
      841 aagaaaatgg aaccgttttc tggagggaaa tttttgatca cacatacgtc ggcgattaag
      901 aggcatcgtc aaagcttaaa aaaacgcacc cgtaacgtcg aaactaagtc gcgtctccga
      961 actctgatca aacgagttca tcgcgcgatc gaatcgcaga atcgcgacga ggctagaaca
     1021 caaatcagtg tcgttaataa ggctctctgt aaggctgtga gcaaaggcat tatcaaacgg
     1081 aatacggcat cccggtggtt ggctcgcctt tcccgttccg ccgcacgggc atccgcttag
     1141 cacgccgact attgttgatc tagcgttatc cctgatcggc tagctcgcgt tcagaacatt
     1201 tcgaagattc gctgccggag ttccctggcg aactgctcca tcaattgcgc ccgaacagct
     1261 ctgtgctcag actcggtgag ctggatgtcg gtcatgcgcc gtacgtccga tacatttaat
     1321 gtgccggtgc gaaattccga ggatgtggtc acgactgctg ccctggaacc accatagacc
     1381 tggttgaacc gcacgccctg cccacgccac aagatttcgt cgggctccct ccgccgcact
     1441 gttatatcca gcatcataac cgcatcgtat tgcagaacct cgtcctctct attcactgtc
     1501 gccactgtac tgtccagcga ccgaatgacc ccgctgagaa tcacatcggc ctggtcgcta
     1561 ctgtcgacca cccggagctc gccatgccga taaaactctc cacgcgttgc agtggtcagt
     1621 tctctctcta tgccgacatc gcggctacgg ttaataaacg gttccacgta aatagtgcgc
     1681 gcgtccttcg gcagcaatga gctttcccca acgaattgat agccgcaccc gctggccagt
     1741 gacacagtca gcactcccag ccaacctccc gaccaccgta agaccttgtg aagtttttgc
     1801 acagtcaccc ctccacgacg atattgacaa gccgacgcgg cacgtagact acgcgccgca
     1861 cggtcctacc atcgatgaaa ccggttacct taggatccgc cagcgcgctc ttctcgatat
     1921 actcttgagt gacttcggcg ggaaccgtaa tttttccccg tatctttccg ttcacttgaa
     1981 cgacgatcaa gaactcttct tccgctagcg catcttcgga ataggccggc caagcggttt
     2041 cgagtgatcc atctttgtgt cccaattctt gccacaactc atgagccacg tgcggaacaa
     2101 acggcgtcag cagcgcaata atagtttcta cgccttcttt gaccaccggc ttggcgcttt
     2161 cggatccgaa ttccgacgcc agcgcgctta cggcattgaa caattccatg attgccgcaa
     2221 tcgccgtgtt gaaatgaaac ctgccatcaa tatcatcgct gacttttttg atagtccgat
     2281 gcgtcatccg gcgtagatcc cgcagttccg cggttaattc gcccggctcg ctagtgcttg
     2341 caatgctcga ccagatcgtc cgatgctggt aaacgaagcg ccacagccga gagagaaaac
     2401 gataggcgcc ttccacaccc tgatcgctcc attccagatc cttttccgga ggagcagcaa
     2461 agagcgtgaa taaacgggcc gtatcggcgc cgtacagatt aaccagatct tccgggtcaa
     2521 tgatgttttt cttcgacttg gacatttttt cgactcggcc tttttccacg ggccggccgc
     2581 actgtgggca gttccatcca tccttttcgg acccggccgg ttctccggga aacaaccagc
     2641 cgtgttcggg acagcgatag gtctctttcg agaccatccc ttgcgtgagc aaattggtga
     2701 acggctcgcc ggttctggtc aggccaagat cacgcaacgc tttggtaaaa aaccgtgcgt
     2761 acagcagatg gagaaccgcg tgctcgacgc cgccaatata ttgatcaact gccatccagt
     2821 agtcgccctg acctttgtca aacggcgcat cggcgtaatg cggggatgca taacggagaa
     2881 agtaccacga ggaatcgacg aaggtgtcca tcgtatcggt ttcgcgccac gcgcgtccgc
     2941 gacattttgg acaggccact tcgacgaaac tcttactttc gcgaagaggc gaacccccct
     3001 ttccggtaaa cggcacatct tcgggaagtc tgaccggcaa ttgctcttcg ggaacgggaa
     3061 ccgtcccaca gcgatcacag taaataatcg gtatcggcgt accccagtaa cgctgccgcg
     3121 acactcccca gtcgcgcagg cgataacgaa ttgctttctt gccccagccg ttcgcttccg
     3181 cacgggcggc gatttgctct tttcccgctg tattcgtaag ccaggaaaaa tcgccactgt
     3241 ctatcatcac gccgtcatca acataagcct cgctcaagct ctcggccttg agcttctggt
     3301 ttaccggttg aatcactacg cgaatcggca gccgatattt cttggcgaat tcaaaatcgc
     3361 gctggtcatg tgccgggaca gccatgacgg cgccggtgcc gtattccatc agcacgaagt
     3421 tggcgagata aatgggaatg ttctcttggg taaacggatt cctacaataa gcgccggtga
     3481 agaccccttc cttttcgcct tcctccgcac cgcggttggt ttggctgacg tttttgaagc
     3541 gatcgataaa atttctgact gcagattcgt gctgagtgcc cgccgccaaa gtgagcgcca
     3601 tcggatgctc gacggcgagc gaaacgaagg tcgcgccgaa gatggtatcc tggcgtgtgg
     3661 taaaaatacg cagcgcaccc tcacgtccaa ccaaaggaaa atcgatttct gcgccgaccg
     3721 atttgccgat ccagttccgc tgcatgatca gaactttgtc aggccaaccg gggagctgct
     3781 ccaaatcccg gagcaattct tcggcgtact cggtgattcg aaaaaaccac tgctcaagct
     3841 ctttctgtgt cactgccgag tcgcagcgcc agcaacgccc atcgatcacc tgctcgttgg
     3901 ccagcacggt ctcgcaggac ggacaccagt tcacaaacga gttttttttg tatgccaaac
     3961 cctttttgaa gaagtgcaaa aaaatccatt gattccactg at
//
LOCUS       DASULZ010000634         2519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_274947, whole genome shotgun sequence.
ACCESSION   DASULZ010000634 DASULZ010000000
VERSION     DASULZ010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274947"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VFY96_13080"
     CDS             <1..579
                     /locus_tag="VFY96_13080"
                     /inference="COORDINATES: protein motif:HMM:NF025221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HEX5607449.1"
                     /translation="LQEALKVGTENAVVQTGTVDGFLKNKAIKILMPKSLQTIEQPLR
                     LVGYGPQIDEFVVGMNRAAEKAVPFAKDIFWEAIGAMTFDDARRILDGNDTAATDYFK
                     GKTSKKLQAAFLPNVKDVMDQVGVNRQYNELIGKYKDVPFSKNIAFDANQYVTEKTTD
                     GLFFVVGQEEKKIRTNPSARVTDLLKEVFGKR"
     gene            complement(610..1329)
                     /locus_tag="VFY96_13085"
     CDS             complement(610..1329)
                     /locus_tag="VFY96_13085"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607450.1"
                     /translation="MYGWRAKIGHVAPSRGDTLVYEFYKMLPEGFMILNSTGTIRQLV
                     DADFERQLQRIEEAAADLVDNKCDSIIIGGSPLFTKLGHGSDIEMSSKLTAKFGVPVS
                     PGITGEVEALKSLNIRQLVVATPHEDALNERMKVFLEASGFKVLKIQGYGIRKNADLT
                     DMDIHSAYKIAKRLYEQALEADGVFVPCPRWPTITDVDLLEREIGKPVVTSCQAYIWH
                     ALKMAKVKQEVKGFGRLMASL"
     gene            complement(1473..2282)
                     /locus_tag="VFY96_13090"
     CDS             complement(1473..2282)
                     /locus_tag="VFY96_13090"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5607451.1"
                     /translation="MLAQRNSTGVIDPAAIIDPNFMKALDNRVAIVTGGGGGIGGAIA
                     QRFSREGAKVAIVDIDAAAAGHCAHQIGLDGSAMSLTADMTKKQAVKEIVTATLNCWG
                     RVDILVNVAGGAERKPVIQMTATDWDRVVDMNLKSVFLCSQAVLPAMVKQRYGKIVNI
                     SSIYGFTGNATRASYAAAKAGVAAFTKSLALEHVQDGLNINAIAPGRIETPRVRGHYS
                     DEVWAAEVAKIPMGRPGTPEEIASTALFLVLDENKYITGQTIHVNGAWLNH"
BASE COUNT          569 a          650 c          689 g          611 t
ORIGIN      
        1 ctccaggaag cattgaaagt gggcaccgaa aatgcggttg tgcagacggg caccgtggat
       61 ggatttctga aaaataaagc gattaagata cttatgccca agtcactgca gacgattgaa
      121 cagcctctgc ggcttgtcgg ctatggaccg cagatcgatg agttcgtcgt cggtatgaat
      181 cgtgccgcgg agaaggccgt gccttttgcc aaagatattt tctgggaggc gattggcgcg
      241 atgacattcg acgacgcccg gagaattctt gacggaaacg acacggcggc cactgactat
      301 ttcaaaggca agacttcaaa aaagctccaa gccgcattcc tgccgaacgt caaggatgtc
      361 atggatcagg tgggcgtcaa tcggcagtac aatgagttga tcggcaagta taaggatgtg
      421 ccgttctcga aaaatattgc attcgacgca aaccaatacg taacggaaaa gacaactgac
      481 ggactgttct tcgttgtcgg gcaagaggag aaaaaaatca ggaccaaccc gtcggcccgc
      541 gttacggatc tcttaaagga ggtcttcggc aaaaggtgag tcgctcagtt ttcagcgagt
      601 gactcatttt tatagggatg ccatcaggcg accaaagccc ttcacttcct gtttcacctt
      661 cgccattttc agggcatgcc agatatacgc ctgacagctg gtcacgacgg gtttgccgat
      721 ttctcgctcg agtagatcaa cgtcggtgat tgtcggccag cgcgggcagg gaacgaatac
      781 gccatcggct tcgagcgctt gctcatagag tcgcttggcg attttatatg cggagtgtat
      841 gtccatatcc gtcagatcgg cgttttttct gatgccgtaa ccctgaattt ttaaaacttt
      901 aaagccggaa gcttccagga aaactttcat ccgctcgttg agcgcatctt cgtgcggtgt
      961 cgcgaccacc agttgcctaa tgttcaatga ctttagagct tctacttcgc cggtaattcc
     1021 cggtgacacc ggaacgccga actttgcggt gagtttgctg ctcatttcaa tatcgctgcc
     1081 atggccgagc ttcgtaaaca gaggcgagcc gccgatgatg atcgaatcgc acttgttatc
     1141 gacgagatcc gcggccgctt cctcaatgcg ctgcagttgg cgctcgaaat cggcatcgac
     1201 aagctgccgg atcgtgccgg tgctgttcaa gatcataaat ccttctggca gcattttgta
     1261 aaattcataa accaacgtat cgccgcgcga tggggcgacg tgaccaattt ttgctcgcca
     1321 gccgtacatt tttctaacct cccccggatt ttatcctagc gctgtcgttt tgagctatgc
     1381 ccaatgttct actgcaagca acatttttcg ttcaaaacat tccaaccgtt ccagtcgttg
     1441 aagtcgttgc cgcacaagaa caaagcaacc ggtcaatgat tgagccaggc accgtttacg
     1501 tgaatggttt gtccggtaat atatttattt tcatcgagaa cgaggaagag cgcggtgctg
     1561 gcgatctcct ccggtgtgcc cgggcggccc attggaattt tggcgacttc ggcggcccag
     1621 acctcatcgg agtaatggcc gcggactcgc ggtgtttcga ttcgacccgg cgcaatggca
     1681 ttgatgttga gcccgtcttg aacgtgttcg agcgccagcg atttggtaaa tgcggcgacg
     1741 ccggctttcg ctgcggcgta gctggcgcgg gtggcattgc cggtgaaacc gtagatcgag
     1801 gaaatattga caatcttgcc gtagcgctgt ttgaccatcg cgggtagcac ggcctgggaa
     1861 cagagaaata ccgatttcaa gttcatatcg acgactctgt cccaatctgt cgcagtcatc
     1921 tggatcaccg gtttgcgctc cgcgccaccg gccacattga ccagaatgtc aacacgcccc
     1981 cagcagttta gagttgccgt gacgatctct ttcaccgcct gttttttcgt catgtcggct
     2041 gtaagagaca tggcgctgcc gtccagccca atctgatgcg cacagtggcc ggctgctgcg
     2101 gcatcgatgt cgacgattgc gaccttggcg ccttcgcggg aaaatctttg cgcaatggct
     2161 ccgccgatgc caccgccgcc gccggtcacg atggcaactc tattgtccag tgctttcata
     2221 aaattcgggt caatgatcgc tgcgggatcg atgaccccgg tgctgttccg ctgcgcaagc
     2281 aagtgaagaa cgggcggaat gaaatcgcga gggcgccgtc gatgtcgagt ccaataagca
     2341 tgaacgtcgt ccaatgtaag caaatcgctc tctggcgtca actttgtgag aggcgtggtc
     2401 gtgttgtcga ctgaatcgct cgatgaggcg gcgctcctat tttcgagccg cgttgaagct
     2461 cgggaatttt cgcgcagtag atttaaggga gctataggat gacatggaga aatcgatta
//
LOCUS       DASULZ010000635         4474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_985500, whole genome shotgun sequence.
ACCESSION   DASULZ010000635 DASULZ010000000
VERSION     DASULZ010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985500"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VFY96_13095"
     CDS             <1..266
                     /locus_tag="VFY96_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607452.1"
                     /translation="YVRLSVLQKYGGKSGVHASGDFTRAATINWYIDCSEQGNNGPTL
                     GPRTVRRSEFEEIPDIRACVQPFVTHHSEYDVGLSAAGRIIEH"
     gene            1091..1555
                     /locus_tag="VFY96_13100"
     CDS             1091..1555
                     /locus_tag="VFY96_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEX5607453.1"
                     /translation="MKRISLAIAVAVGAFLVAAITSFAQPRQGMMWRGSGGWGPGTPY
                     NRMYDPKAMETISGEVISIDRITPNKGMAAGIHMNVKTDKETISVHLGPSWYLENQDV
                     KIEPKDTVEVKGAKTTFAGKPAIIAAEVKKGDETLKLRDESGFPVWAGWRRR"
     gene            complement(1916..2809)
                     /locus_tag="VFY96_13105"
     CDS             complement(1916..2809)
                     /locus_tag="VFY96_13105"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5607454.1"
                     /translation="MSSVKFSAKVWNSTAACVVVVIVMAAQLFGAAWLQEPVQPRIEE
                     EFTKQEEIYSKRGPGSYTTNRGLSSYAELLPSGFCDALDRLGSSDRWLDIGAGQGLAI
                     LDYYTARQETAPEKCGGSGAKASVVAISIEDRRTEKWKQQAPILGENRIRYLSGKRLS
                     QYSLEELGKFQLITDVYGGFTYTDNLSRFIEKALSLLEIGGAFYTVLSSVHLENGKEK
                     LGSWYRTELVDAASRPVTVCSWLKQTTCTKVECESKSDWAEPSELITIRKVCTGVIVP
                     RLNLVEFTAGSPPGRRFQLEP"
     gene            3165..3413
                     /locus_tag="VFY96_13110"
     CDS             3165..3413
                     /locus_tag="VFY96_13110"
                     /inference="COORDINATES: protein motif:HMM:NF019459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HEX5607455.1"
                     /translation="MSPFRSNPAAYFGRGSRGTKEFQRHVYCRFGILLLIATPVARVG
                     VSIFAFIYQRDRLYTLITATVFCLLVIGCPWQSRVAPN"
     gene            3438..>4474
                     /locus_tag="VFY96_13115"
     CDS             3438..>4474
                     /locus_tag="VFY96_13115"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607456.1"
                     /translation="MLVARILVVFCCFLVSVEARAQTQLRFVTWRSEATRVFQQIIAE
                     FESSHPGIKVVQEIGPSSSTEFHDLVTQKLRNRDAEMDVFFMDVVWPAEFAAAGWALP
                     LDGFFPRSAQNDFLDAPIRANSYAGRIYGVPMFVDAGMLYYRKDLLAKYQLRPPDTWP
                     ELVRQARFIISQERDPYLTGYSAQFKQYEGLVCNMMEFILSNGGALWDEQRLVSSVHT
                     AKAMEAVRFVRDDIVRLIANRGILAYQEPESLALFTQGRAVFHRNWPYAWEAANDPGQ
                     SKVAGKVGVMPLPAFSGQKSAATLGGWQLAISRYSRRAQLAWEFVQFMTRSETQKRIA
                     LGTGRAPARKDL"
BASE COUNT          957 a         1189 c         1263 g         1065 t
ORIGIN      
        1 tttatgtccg attgtcagtg ttgcaaaaat atggcggcaa aagtggcgtt catgcgagcg
       61 gcgattttac acgggcggca accatcaact ggtatatcga ttgctctgaa caaggtaaca
      121 atggaccgac attagggccg aggacggttc gccgctctga atttgaagag atacctgaca
      181 tccgcgcttg tgttcagccg tttgtgactc accattcgga gtatgatgtt ggtttgagcg
      241 ccgccgggcg aattatcgag cattgatcta gggaaaagaa ggttaaatcc atgtctgact
      301 ggttgtggtt catcgcgttc ttttgcgtgt acgtcgtgct tatgcgttgg gtgctgcccg
      361 cgctgggcgt gccgacctga atgtcggcgt catgcgacgt cgagtctcga cgcagaactg
      421 acgatgatca agagcatgaa aggacaaagc gcaagaagta acccgattcg atcgaaatca
      481 aatttaagcc aatcggcaca caaatgcgag atcaaagaaa caggtgatcg ggaaagtgtg
      541 ctggaggttt ttccagaatt tgcagcgggg cttgatggca ttgacggcta ttcgcatttg
      601 gtttgtcatc gtctagttcg atggcttgcg tcgcgatcaa atcggaccgt taaaggtcaa
      661 gccacgccgc cttttcagcg ggggttcacc ttgatgagtt gcctctgctt ggcgagttgc
      721 cctcgactcg acgcatccta agcctattag cgtcactttg ttgcgcgtgc tcaagcgcga
      781 tggcaatcgg atttccgtgc gcggtttgga tttcttcgac ggcaccgctc ctcgatatca
      841 aagggtatcg acctcagtat cgcgcagacg attacacttt cccccaatgg ttccgcaaat
      901 tggcggacgc caaaggtcat gtctgaagcg actgattcgc gttcctggag atgcatcaac
      961 cttagcttcg caattttgca aatcaacgag ctaaaccccc gataacgacc gattgcaaac
     1021 ggcatgtgca ttgctcatac gactaagcgc ctagtactcg aaaaacccaa aagagaggag
     1081 gtgagaacaa atgaagagaa ttagtttagc aatcgcggtt gcagtgggag cgtttttggt
     1141 tgctgcaatt acatcatttg cccagccgcg gcagggaatg atgtggcgag gaagcggtgg
     1201 ctggggaccg ggaacaccgt acaacaggat gtatgatccg aaagccatgg aaacgatttc
     1261 cggcgaagtg atcagcatcg atcgcatcac gcccaacaaa ggaatggcag ctgggattca
     1321 tatgaacgtg aagacggaca aagagacgat ttccgttcac cttggcccga gctggtattt
     1381 ggaaaatcag gacgtgaaga tcgagcccaa ggatacggtg gaggtcaaag gggcgaagac
     1441 gacctttgcc ggaaagccag cgatcattgc cgccgaagtg aagaaaggcg atgagacatt
     1501 aaagctgcgt gacgagagcg ggtttccggt ttgggccggc tggcgacgac ggtagctctc
     1561 gcagtgggcg cccgagggtc agcggcgaga aggaaacaaa acgtcctttt tgccgttggc
     1621 tccattcttg ttttgcaacg aatggattgg ccgcgcaaga agaagggcgt taagggcagg
     1681 cttgcttgtt gctcggcctg ctggcgcagt tgttacggct gatattagct tatacttgaa
     1741 attgaagtac tccgcgcgtc gcgccatcct gcgcgttgat ttccactgac cctgttgcag
     1801 cgaatgctca ttcgcagaga tgagtacaat tgcgcattag caattgatgg cttgctattc
     1861 ccgtccgcgc atcatgtggg cgcgcgctga gctaaactgc gcccacgcct accgcctacg
     1921 gctctaattg aaatcgcctg ccgggaggag atcccgccgt aaactccaca agattaagcc
     1981 gcgggacgat cacaccggtg caaaccttgc gaatggttat cagctcgctc ggctcggccc
     2041 agtcgctctt cgactcgcac tcgacctttg tgcaagtggt ctgcttgagc caggagcaca
     2101 cggttacggg ccggctcgct gcatccacca gctcggtgcg ataccagctg ccgagttttt
     2161 ccttgccatt ctccagatgc acgctcgaca gcactgtata gaaggcgccg ccgatctcca
     2221 gcaggctcaa cgccttctcg atgaaccggg acaaattatc ggtgtaagtg aagccgccgt
     2281 atacgtcggt gataagctgg aattttccca gctcctccag cgaatattgg ctgaggcgct
     2341 tgccggagag ataccggata cggttctcgc cgagaatcgg ggcctgttgt ttccacttct
     2401 ccgttcgacg gtcctcgatg gagatcgcga ccacgctcgc tttggcgccg gacccgccgc
     2461 acttttcagg agccgtctcc tgtcgcgctg tgtagtagtc cagtatggcc agcccctggc
     2521 ctgcgccgat atcgagccac cggtccgagc tgccgagtct gtcgagcgcg tcgcagaagc
     2581 cgctgggaag aagctccgcg tagctcgata acccgcggtt cgtcgtgtag ctcccgggcc
     2641 cgcgtttgct gtagatttct tcctgtttgg tgaattcttc ttcgatccgc ggttggacgg
     2701 gttcctgtag ccacgcagcg ccaaataact gcgcggccat aacgatcaca acaaccacac
     2761 acgccgccgt agaattccat actttcgccg aaaatttgac tgaactcatg gcgattatat
     2821 tctcctcgcg cttataaaac tcccgttctt taaaatatgc tctggagttt gctccatatc
     2881 ttcggttgcc cgaaaaagct cgatgggcgt gttccgtttg agtacctcgc tccgtaccgc
     2941 ggtcctgaat acactttcag aagcttcggt caataacccg gcgtgctgct tcggttggga
     3001 aagctcttcc cacggctctt attcttcgct cggcgcccgt ttccgcctga gcgacctctt
     3061 tgaggtttgg ctgcaggacc gctttggcgt gatctcgtct ttgcgagaaa tcccagttat
     3121 gatgatcgaa atggcgcgtc agagatatcg gaggcggaac gttcatttca ccgtttcgtt
     3181 ccaacccagc agcatacttt ggcaggggta gcagagggac taaagagttt cagcggcatg
     3241 tctattgtcg ttttggaatc ttgctactca tcgctacacc agtggcccgc gtcggtgtct
     3301 cgattttcgc cttcatctat cagcgcgacc ggctttacac tttgatcacc gcaaccgtgt
     3361 tttgcttgct tgttatcggt tgtccttggc aaagtcgagt agcgccgaac tagtcggcgg
     3421 gaaattcgtg ttgactcatg ctggttgcga ggatactcgt tgttttctgc tgttttctcg
     3481 tctccgtaga ggctcgcgcg cagactcagc tgcgctttgt gacctggcgc tcggaagcca
     3541 cgcgtgtttt ccagcagatc atcgccgagt tcgaaagcag tcatccagga attaaagtcg
     3601 tccaggagat cggcccgagc tcctcaaccg agttccacga tctcgtcact caaaaattgc
     3661 gcaaccgtga tgccgagatg gacgtctttt tcatggatgt tgtctggccg gcggaatttg
     3721 ctgccgcggg ttgggcactg ccgctcgatg gtttttttcc gcgctccgcg caaaacgatt
     3781 ttttggatgc gccgatcaga gccaatagct atgcaggacg catctacggc gtgccgatgt
     3841 ttgtcgatgc cggcatgctt tactatcgca aggatctgct cgccaaatat cagttgcgcc
     3901 cgccggatac ctggccagaa ctggtgcggc aggcgcgatt catcatcagt caagagcgcg
     3961 atccctatct caccggttac tccgcacaat tcaagcaata tgagggcttg gtgtgcaaca
     4021 tgatggagtt tattttaagc aatggcggcg cgctctggga cgaacagcgg cttgtaagct
     4081 ctgtgcatac ggcgaaggca atggaggccg taagattcgt gcgcgatgac atcgtccgcc
     4141 tgatcgcaaa ccgcggcatt ctggcctatc aagagcccga atccctggcg ttgttcacac
     4201 aggggagggc agtttttcac cgcaattggc cctacgcctg ggaagctgcc aatgatccgg
     4261 gccagtcaaa agtagcgggg aaagtcggtg tcatgccgtt gccggcgttt tctggccaga
     4321 aaagcgcggc gacgctggga ggatggcaat tggcgatcag ccgatattcg cgccgtgcgc
     4381 agcttgcctg ggaattcgtc caattcatga cccgcagcga gacacaaaag cgcattgcgc
     4441 tcggtaccgg ccgcgcgccg gcgcgcaagg acct
//
LOCUS       DASULZ010000636         4444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1085808, whole genome shotgun sequence.
ACCESSION   DASULZ010000636 DASULZ010000000
VERSION     DASULZ010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085808"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VFY96_13120"
     CDS             <1..243
                     /locus_tag="VFY96_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607457.1"
                     /translation="FGAVRDPIRSLMETAVSRDVRTVVVDGEILVDQGKYLRLDEQEL
                     LENVQIKGEQVWQSVNKWHWTGKGIDEIVPPSFKMR"
     gene            complement(277..2061)
                     /locus_tag="VFY96_13125"
     CDS             complement(277..2061)
                     /locus_tag="VFY96_13125"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEX5607458.1"
                     /translation="MASSAEGIRWKLNDLFRAADDPEIENTLADYRARAQAFAKRVRP
                     LMQNPQTLDVEDLLQALTELENIYDALGRVGSYAGLLYAADTANPVHQDLEQKVEQRS
                     TEIRNLLLFFELEWLKFDDTVADRLLENERLKSYAHYLRNLRRYRPHTLAEPEEKIVN
                     EKDNTGRNAFGRLFSEITSALLFTLDIDGKQEPRNLSQMLSLLHEPDRTLRQHAMETL
                     YRGLSEHGQVLSFVYDTLIQDHLTMDRLRRYADPMAQRHLSNEIDPSAVKIMMEVTEE
                     HYHLAQDYFRLKAKLLKLPRIALYDQYAPLGKEARDFRYAQAQELILEAFETFDPSFR
                     QMAAEFFANSWIDAEIRNGKRGGAFCASPSPELHPYILCNYDDHLRDAMTLAHELGHG
                     LHGCLSRKQNYFNYDTPLTTAETASIFSEMLVFDHLLPREPDRQAQLALSAGKIEDIL
                     ATVFRQNVLTRFEELAFAARQDRRLTAAALGNLWMDANRKYYGEAVEMVDGYRWGWSY
                     IPHFIHSRFYCYSYVFGQLLVLALYRMYKDQGKSFVPKYLALLEAGGSDSPEALLRPL
                     GVDIHDREFWRKGFAEIAGMIATFQKLI"
     gene            2294..3004
                     /locus_tag="VFY96_13130"
     CDS             2294..3004
                     /locus_tag="VFY96_13130"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX5607459.1"
                     /translation="MSDVAELKIKLAMSSRMLFNAGLVDYSGHISVRIPGSDHLLILP
                     HPVSRATVKPDEMVITDFEGKLIEGKYKAPSEVYMHARAYKARGDVQSVAHLHNHMVA
                     TLSMVDKPFYPASSNPGAFFGPGALPKYMDPALIHTNEQGDAVAKALGTADAVMLRGH
                     GSMVVGQAIEWVFAACVDLEEAASRFYKAALLGPVLVYNEDETQRVMKGRRKDSVVQK
                     IWDHNAAKTKLAGLMDGI"
     gene            3192..3566
                     /locus_tag="VFY96_13135"
     CDS             3192..3566
                     /locus_tag="VFY96_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607460.1"
                     /translation="MYDDFLARILEPDIFLPSQFYGAGGLSRQLEGEKRLMIAVLKDA
                     VECLDKYRGVRSPLGRCQYQSAIEWVQDTDTDWLFSFTNICDLLGFDPEYLREMLLKR
                     ENKYVKPARPKILSLAAMAAEH"
     gene            complement(3567..>4444)
                     /locus_tag="VFY96_13140"
     CDS             complement(3567..>4444)
                     /locus_tag="VFY96_13140"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5607461.1"
                     /translation="VSATRQLAYSRQLEMEADTLGTRYMAAAGYDPKGTIAFLKMLDQ
                     ERALNPIDVPAYVLSHPITQERVANAELVVKSLGSTQLRSDDPEALKKVQIVIRMDRR
                     DEVVHEYEKLLKQAPRNPEFLYLLGYAQQLQGQLSEARASYEQARKIWPENAGLQRDL
                     GRLYAEIGDFANSRTAFEQSLATQPNEPLTYLYLGEMLERSGDLRSAVGSYLNAQNLA
                     PLWDRPFYKLGMLYGKLERPGDGYYYLGRAFALQDEDDKAIKNLERAVKILGANSPRG
                     QIIQEELAMLKKRKR"
BASE COUNT          990 a         1134 c         1269 g         1051 t
ORIGIN      
        1 ttcggtgccg tacgcgaccc catccgctcc ttgatggaga ctgcggttag tcgggatgtg
       61 cggacagtgg tcgtggacgg tgagatactt gtcgatcaag gaaagtacct tcgcctggac
      121 gaacaagagc tactcgagaa cgtgcaaatc aagggggaac aggtttggca gagcgtcaac
      181 aagtggcatt ggacgggaaa gggaatcgat gaaatcgtgc ccccgagttt caagatgagg
      241 tgatccgagt ttccctgagc tgttccggta taccgatcag attaacttct gaaaagtggc
      301 aatcattcct gcaatttctg cgaatccctt tcgccaaaac tctcgatcgt gaatgtcgac
      361 gccaagcggc cgcagcagcg cctcgggcga atccgaaccg ccggcctcga gcagcgccag
      421 atattttggc acgaaactct tcccctggtc tttgtacatg cggtaaagcg cgagcaccaa
      481 gagctgacca aacacgtagc tgtagcaata gaaccgtgag tggatgaaat gtgggatgta
      541 ggaccatccc cagcgatatc catcgaccat ttccactgcc tcgccgtaat atttccgatt
      601 cgcatccatc cagagattgc caagcgctgc cgcggtcaag cgtcgatcct gacgggcggc
      661 gaaggcgagt tcctcaaacc gagtgagcac attctggcgg aacaccgtgg ccaagatgtc
      721 ctcgatcttg ccggcgctga gagcaagttg tgcctgacga tccggctctc tgggcaggag
      781 gtgatcaaac accagcattt cgctaaaaat ggatgcggtt tcggcagtgg tcagcggcgt
      841 gtcgtagttg aagtaatttt gtttacggct caagcagcca tgcagcccgt gaccaagctc
      901 gtgggccaag gtcatagcgt cgcgtaaatg atcatcgtag ttgcaaagaa tataagggtg
      961 cagttctggg gaaggcgatg cgcaaaacgc gccgccgcgt ttgccgttgc gtatctcggc
     1021 gtcgatccaa ctgttggcaa aaaactccgc ggccatttgc cgaaaggatg gatcaaaggt
     1081 ttcgaacgcc tcaaggatga gctcctgtgc ttgtgcatag cgaaaatcgc gagcttcctt
     1141 tcccagcggc gcgtattgat cgtaaagcgc gattcgtgga agtttgagaa gcttggcttt
     1201 gagacggaag taatcttgag ccaagtgata gtgctcctct gtcacttcca tcatgatttt
     1261 cactgcgctg ggatcgattt cattcgacag gtggcgttgc gccatcggat cggcgtaacg
     1321 ccgcagtcga tccatggtta aatgatcttg aatgagagtg tcgtaaacaa aacttagcac
     1381 ttgaccatgt tcggagagtc cgcgatagag cgtttccatc gcgtgttggc gcagtgttcg
     1441 atcgggctcg tgcagcagcg aaagcatttg actcaaattg cgcggttcct gcttgccgtc
     1501 gatatcgagc gtaaacaaga gcgcagaggt tatttcggag aatagccggc cgaaggcatt
     1561 acggccggtg ttgtcttttt cattaacgat tttttcttcc ggttcggcga gcgtatgcgg
     1621 gcgatagcgc cggagattcc gaaggtaatg cgcgtagctt ttcaaccgct cattttcgag
     1681 gagccgatcg gcgactgtgt cgtcgaattt tagccattcg agttcgaaga agagcaataa
     1741 attgcgtatc tcagtcgagc gttgctcgac cttttgctcc aaatcctgat gcaccggatt
     1801 ggcggtatcc gcggcgtaaa gtaatccggc atagcttcca actcggccca aagcgtcgta
     1861 gatattttcc aattcggtga gcgcttggag cagatcctcg acatcgagcg tttgcgggtt
     1921 ttgcatcaag ggtctcacgc gtttggcaaa cgcttgcgca cgcgcacggt aatcggccag
     1981 ggtgttttcg atctccggat cgtcggctgc tctaaagaga tcattgagct tccagcgaat
     2041 tccttcagcg ctgctcgcca tgtaatggtc ctttttaggt gttcggttct atcttatcgt
     2101 cacacttcgt cggctttgct cctcgaaatc gaccggagtt ggcccaatca ttccgacctg
     2161 ggtctccgga ggcaggccgc aattgactag gccgtggtgt gaagcttcgc aacgcggcgg
     2221 cggaagttgg gttgaactcc gatggaatga aagatatagc acaaaaaagc agcttctgga
     2281 gaggagaaac ttcgtgtcgg acgtagcgga actcaaaatc aaactcgcca tgtcgtcgcg
     2341 tatgttattc aatgccggat tggtggatta cagcgggcat atcagcgtgc ggattcccgg
     2401 ctcggaccac ctcttgatct tgccccatcc ggtgagccgc gcgacggtga aaccggacga
     2461 aatggtcatc acggatttcg aaggaaagtt gatcgagggc aagtacaaag cgccctctga
     2521 agtatacatg catgcccgcg cttacaaggc gcgcggagat gtccagagcg tggctcactt
     2581 gcacaaccat atggtggcca cgctgtcgat ggtggataaa ccgttctatc ccgcatcttc
     2641 aaacccgggc gcattcttcg gaccgggggc actaccgaaa tacatggatc ccgcgctaat
     2701 tcatacgaac gagcagggag acgcggtagc aaaagcacta gggacggccg atgccgtcat
     2761 gctgcgcggc cacggttcca tggttgtcgg acaggcgatc gagtgggttt ttgccgcttg
     2821 cgtggacttg gaggaagccg cctcgcgatt ctacaaagcc gctctgctgg gtccggtgct
     2881 agtatacaac gaggacgaga cgcagcgcgt catgaaaggc cggcgaaagg actcggtggt
     2941 gcagaaaatc tgggatcaca atgcggccaa aaccaaactc gccggcttaa tggacggcat
     3001 ttagcgcccg agcggttctc atcgctagat ttaaacgaat cagcagcttt tggttccgtc
     3061 cgcaaggcct cacggcgggc ggttcatatt ggtttgagcg ggtgaattgt catgccatag
     3121 agaatttatt gcgtggcgat ttcacctgtg gtatagaatg cgattcaaat tgatgaggag
     3181 agtgtgcggc gatgtacgat gatttcttag cgcgaatcct ggaacctgac atctttttgc
     3241 cgtcgcagtt ctacggcgct ggaggtttat cccggcagct cgaaggcgaa aaacgcctca
     3301 tgatcgcggt cttgaaggat gccgtggaat gtctcgataa gtatcgcggt gttcggagcc
     3361 ctttgggtcg gtgccagtat cagagcgcca tcgaatgggt tcaagacacg gataccgatt
     3421 ggctgttttc gtttaccaat atctgcgatt tattgggttt tgatccggag tacttgcggg
     3481 aaatgctgct caagcgggaa aacaagtacg tcaaaccggc gcgtccgaaa atattgtctc
     3541 tggccgctat ggcggccgag cattaattag cgttttctct ttttcagcat agcaagctct
     3601 tcctgaataa tctgacctcg gggcgagttc gccccgagga ttttgaccgc gcgctcaaga
     3661 tttttgatcg ccttgtcatc ctcatcttga agagcaaagg ctcggcccag gtaatagtag
     3721 ccgtctccgg gccgctcgag cttgccgtag agcatcccca gtttgtaaaa cggacgatcc
     3781 cagagcggcg ccaaattttg cgcattcaga tatgacccga ccgcgctgcg caaatcgccc
     3841 gacctctcga gcatctcacc caaatacaaa tacgtcagtg gttcgttagg ctgggtggct
     3901 aacgactgct cgaatgcagt tcgggaatta gcaaaatcgc caatttcggc gtagagccga
     3961 cccagatcgc gttgcaagcc ggcattctcc ggccaaattt ttcgggcctg ttcgtagctt
     4021 gctcgcgcct cagatagctg cccctgaagt tgctgagcat agccgagcag gtaaaggaac
     4081 tccgggtttc gcggcgcttg cttaagcaat ttttcatact cgtgcactac ttcatcccgg
     4141 cgatccatgc ggatcacgat ctgcactttt ttaagcgctt cagggtcgtc ggagcgcagc
     4201 tgagtcgaac cgagcgattt gacaaccagt tcggcattgg cgactcgttc ctgagtgatg
     4261 ggatggctta agacgtatgc cggtacgtcg atcggattaa gcgcccgctc ttgatcaagc
     4321 atcttcagaa aagcgatcgt gcctttggga tcgtagcccg cggcagccat gtaccgcgta
     4381 ccgagggtgt ccgcttccat ttcgagttgt cggctgtacg ccagttggcg ggtggccgag
     4441 acag
//
LOCUS       DASULZ010000637         2630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1085819, whole genome shotgun sequence.
ACCESSION   DASULZ010000637 DASULZ010000000
VERSION     DASULZ010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085819"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VFY96_13145"
     CDS             complement(<1..663)
                     /locus_tag="VFY96_13145"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5607462.1"
                     /translation="MAKNGFKIFDSDMHIMEPPDLWERYIAPEFRAQAPRGRTSENVR
                     DLGTIFPTLPKESRRTGGTPHRGRNYNKNQEIYRNHAERGWTGEVQLEAMATEGIDVA
                     VLFPTRGLGILTFPDQDPRFAAAMARAYNDWLHDFCQADPTRLLGAGMISVYDINDAL
                     SETRRVAEEYGFRSVFLRSNIVNGKNWYDPYYEPLWNLLEELSLPLGFHEASASRSRQ
                     CAE"
     gene            779..2053
                     /locus_tag="VFY96_13150"
     CDS             779..2053
                     /locus_tag="VFY96_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HEX5607463.1"
                     /translation="MAFTLALHPITNLTLGDKHRIDRDALTVDVDDLRSMVLADNAIE
                     SVEFEIARPGESCRAGPIFDIVEPRAKAPGGSPDWPGILGPPHTAGFGTTHLLQGTAV
                     TMLREESPGESRGVTGYVLEMSGEAGRASCYSKLWHLIVIPHTKATIPSHTRHRAYRI
                     AQLKLAVRLAQIAMDTPPAETLVLDTFIGPDRVSLTRVAYVGQIFSRQRKPEPEEQII
                     YGANTDGMLPTLLHPNEWLDGAITTSYHTSIGGAETYFYQNNPVIAELYRRHQRGEIN
                     FVGTVATIAAADNLDRERNSQFAAHLVKWALRAEAAVLTKFGGGVPHSDLAETARLLE
                     RNGIKTAVQVTDLARDHRVESALLFNFPEVDAIVCIGGNGTRWKLPRAERVLAANGQM
                     AELLAGPVEIDSLNLIGVANQQGASRLRSVIY"
     gene            2135..>2630
                     /locus_tag="VFY96_13155"
     CDS             2135..>2630
                     /locus_tag="VFY96_13155"
                     /inference="COORDINATES: protein motif:HMM:NF019003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HEX5607464.1"
                     /translation="MRVVHYLNQFFAGIGGEEQAAMPMEIYDGPIGPGKRLEQLFGTE
                     AQIVLSLVCGDNYATENQNEFIASAIKKIGAVDTDLLVAGPCFLAGRYGVAAGALCKA
                     VQAQLGIPVVTGMARENPGADLYRESLYIIDSGESAAKMGEVLTRMARFAQKLSLKQE
                     IGSPT"
BASE COUNT          573 a          689 c          797 g          571 t
ORIGIN      
        1 ctcggcgcac tgccgcgagc gggaggcgga cgcctcgtga aatccgagcg gtaagctgag
       61 ctcttcgagc agattccaga gcggctcata ataaggatcg taccagttct tgccgttcac
      121 gatattcgag cggagaaata ccgaccgaaa gccatactcc tcagcgactc ggcgcgtttc
      181 gctcagcgcg tcgttaatgt catagaccga aatcatgccg gcgccgagca ggcgggttgg
      241 atcggcctga caaaaatcgt gaagccaatc attgtacgcg cgcgccatcg ccgcggcgaa
      301 gcgcggatct tggtcgggga aagtaaggat gccgagaccg cgcgtcggaa acaggacggc
      361 gacgtctatg ccctcggtgg ccatcgcttc gagctggact tctcccgtcc agccgcgctc
      421 ggcgtgattt cggtagatct cttggttctt gttgtaattg cggccgcggt gcggcgtccc
      481 gccggtgcgc cgagactcct tcgggagcgt gggaaagatc gtgccgagat cgcggacgtt
      541 ttctgaagtt cttccacgcg gcgcttgagc gcgaaattcg ggggcaatat agcgctccca
      601 tagatccggc ggttccataa tatgcatgtc gctgtcaaaa atcttgaagc cgttctttgc
      661 cattgcgatt tcccctagtt gccggaagcg taatgccgct caaggtctca tgtcaagcca
      721 atcagccgaa ttgacaagcg aaacgattcg ccactagagt cttttctggt acctcgatat
      781 ggcatttacc ctggctttgc acccgattac gaatttgacg ctcggtgaca aacaccggat
      841 cgaccgagac gcgcttacgg tcgacgtcga tgacttgcga tccatggttt tggcagataa
      901 cgcgatcgag tcggtcgagt tcgagattgc gcggcccgga gagagttgcc gggcggggcc
      961 gatcttcgac attgtcgagc ccagggctaa agcaccaggc ggaagtcccg actggccggg
     1021 cattctcggc ccgccgcaca ctgcggggtt cggaacaacg catttgcttc aaggcactgc
     1081 ggtgacgatg ttgcgagaag agtcaccggg agaatcgcgc ggggtgaccg gttatgttct
     1141 ggaaatgagc ggggaagctg gtagggcttc gtgctattcc aagctatggc acttgatcgt
     1201 cattccacat actaaagcga caatacctag tcatacgcga cacagagctt accgtatcgc
     1261 acagctaaaa ctggccgtgc gattagccca aattgcgatg gatacgccgc cggcagaaac
     1321 actggtgttg gatactttca ttggccccga tcgtgtttcg ctgacgcgag tcgcgtacgt
     1381 cggccagatc ttttcgcgtc agagaaagcc tgagcctgag gagcaaatta tttatggcgc
     1441 caataccgat ggtatgcttc ccacgttgtt gcacccgaac gaatggctcg acggcgcgat
     1501 tacgacgtcc taccacacgt cgatcggtgg cgcagagact tacttttacc agaataatcc
     1561 agtcattgcg gagctctata ggcggcatca aagaggagaa ataaacttcg tcggtacagt
     1621 ggctacgatc gctgcagcgg acaatttaga ccgagaacgg aactctcagt ttgccgctca
     1681 cctggttaaa tgggctctgc gtgccgaagc ggcagtgctg acgaagttcg gtggcggcgt
     1741 gccgcatagc gatttggccg aaacggcgcg actgctggaa cggaacggta tcaagacagc
     1801 ggttcaagtg acggatctcg ctcgtgatca ccgggtcgaa tcggcgctgc tgttcaattt
     1861 tcctgaagtc gatgcgatcg tctgtatcgg cggcaatggc acgcgctgga aattgcctcg
     1921 tgcggagcgc gtgcttgcgg cgaacggcca aatggcagaa ttgcttgcgg gaccggttga
     1981 gatcgattcg ctcaatctca ttggagtcgc taaccagcag ggggcgtcgc ggctgcgctc
     2041 tgtaatttat tagggcaaat aagcgcccag catcggtgta gtggcgcagc atgcgctcct
     2101 tttgctaccg cggtgggaag tcttcggagg atatatgcgg gtggttcatt acctgaatca
     2161 gttttttgct ggcattggcg gagaagagca ggcggccatg ccgatggaga tttatgacgg
     2221 tccgatcggg ccgggtaagc ggctcgagca attgttcggc accgaggcgc agatcgtatt
     2281 gtccctggtt tgcggtgaca actacgcgac agaaaaccaa aacgaattca ttgcctcagc
     2341 gataaaaaaa attggcgcag tggataccga cctgctcgtt gccggtccat gctttctagc
     2401 cggaagatac ggtgttgccg cgggtgcgtt gtgtaaggct gtacaagcac aattaggaat
     2461 tccagttgtc accggcatgg cccgcgaaaa tcctggtgcc gatctctatc gagaaagctt
     2521 gtatatcatc gacagcggtg aaagcgcagc aaagatgggg gaggtgttga cccggatggc
     2581 ccgtttcgcg cagaaactta gcctgaagca ggaaatcggc tcgccgaccg
//
LOCUS       DASULZ010000638         5326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_782163, whole genome shotgun sequence.
ACCESSION   DASULZ010000638 DASULZ010000000
VERSION     DASULZ010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782163"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VFY96_13160"
     CDS             complement(<1..816)
                     /locus_tag="VFY96_13160"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEX5607465.1"
                     /translation="MGMKGIVGIGGCLLGLAIVYFSSGVGTESAKKEVAARATATEPQ
                     ESVKPLRAMNFALDNMVFFAHELGFTVITANDEAVSKNRVATRIENQLHNVRELYRVE
                     IAKNPSLAGVILLQLDITPSGEVSQVRETGSKITDSEFKKAVIAEVAKWSFRDIVSES
                     LKVNCPLLFVHQGMDITTLVRWEKATAGVNEKNMVQPAPMAKGSVRNAPNLPAAVKTT
                     SVPTRVPGNMPSGQVYQIKYATLLRREPNFTAPPLTSYTIGTKVVVQKKLGDWL"
     gene            1165..2187
                     /locus_tag="VFY96_13165"
     CDS             1165..2187
                     /locus_tag="VFY96_13165"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5607466.1"
                     /translation="MIAIILLLLVFDRAELFAQTPYYQGKTITFIVGSGAGTAYDMYS
                     RLLANHIGKHIAGNPTVVVQNMPAAGGMVAANFVYGVAKPDGLTVVSINPAHYFNQLQ
                     GNKEVKFDWPKFTWLASSDKSEHMLYMRADAPYKTIQDVRRAAEPPKCGATGTGTSGH
                     YFPRMLEETLGTRFNIVTGYAGGNEIDLATERNEVVCRAFTTAAYFAREPYHTWRKKN
                     FVRVLLQTGHKRDPQLPDVPLLSDLMDEFKTSELGRRVATVMLGSGELGRPIVTSPGL
                     APDRVKILRDAFMKSMADPALLTDAAKNKLEITPVSGDELAKIARDVIDQPTEVVERI
                     KKVLAQ"
     gene            2276..2803
                     /locus_tag="VFY96_13170"
     CDS             2276..2803
                     /locus_tag="VFY96_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5607467.1"
                     /translation="MRPIRVIFVACAVATFFFNAELFAQSAAKPATVIQAGSLVAFDY
                     TLTDDTGKVIDSSQGKEPMHYVHGKGQIIPGLEKELTGMSAGSEKKVTVKPEDAYGQV
                     NPQAFQEVPKDKLPPEALKVGTVVTARSPEGQSVPVRVHEIKENTVVMDFNHPLAGKT
                     LFFDVKVTEVKPANP"
     gene            2965..3375
                     /locus_tag="VFY96_13175"
     CDS             2965..3375
                     /locus_tag="VFY96_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607468.1"
                     /translation="MTLLLTRSEVATLLDLQRAMLVLEQTFREQSAGQVKPVAPLRFM
                     NRGMRMVVGGLEAQDKNGFRVSVTGGDSLAMLFEISSGKLLCLTGYPFSNLRIDQRAT
                     RRFSHRAGALSGDTGKIQPARILLGTRSDENSLA"
     gene            3524..4708
                     /locus_tag="VFY96_13180"
     CDS             3524..4708
                     /locus_tag="VFY96_13180"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX5607469.1"
                     /translation="MIPQLSIEERDRRYKVVRAEMAQRNLDVLLLPANTGRWEQLQGD
                     SRYLTSIGGFATEVFTVFPRQGDVTAYIFNRAGWWKQAQKWVSDVRDGRNRWGQNAIE
                     RISELGLQKGTIGISGLAGLFRAPEGIIPYSSVKAIQDAFPSAKVVNATEMMQAIRAV
                     KSAEEVSLLERSAAIVEKTIEAMVEGANPGVSEKELYGAMVHAMLANGGELPTLFFLS
                     GGPGISNSSFVPTDYRLQKGDRLIDEIEAKYAGYAAQAVCPVVLGKPDDEYQTMIDIS
                     RACFDAILHQMKPGVTFGLLFEVYRDTVERLGKGKYLWNHPVMHARGLGDDGPALLGD
                     SDLERFSKIELQAGMTFILKPQVCPKEGKGRASIGDTVAVTENSARRLGKRELKLIVK
                     NV"
     gene            4828..>5326
                     /locus_tag="VFY96_13185"
     CDS             4828..>5326
                     /locus_tag="VFY96_13185"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEX5607470.1"
                     /translation="MPELYPYPRFSTSERDRRWQAVRALMRAQNLDVIVTPQNSGHSA
                     DYQANTRYLTHCGGGEPDLAAVFPLEGEVTAIATSAAPRWPTVQDWVFDVREARRNYG
                     RAIVERLKELKLERGRIGITGLGEVEGTRTPEGTIFYGTWKQIREAFPHAELIDATAI
                     LDEVRY"
BASE COUNT         1203 a         1397 c         1552 g         1174 t
ORIGIN      
        1 caaccagtcg ccgagctttt tctgcaccac tactttggtc ccgatcgtat aggaagtcag
       61 cggaggagcg gtgaagttcg gctctctacg cagcaaggtt gcgtatttga tctggtaaac
      121 ttgtccgctc ggcatatttc ccggcactct cgtcggaacc gacgtggttt tcacggcggc
      181 cggtaaattc ggcgcatttc ggacagagcc tttggccatg ggggcgggct gcaccatatt
      241 cttttcattg acgccggcgg tcgccttttc ccaccgcacc aaagtcgtga tgtccatccc
      301 ttgatgcacg aagagcaagg ggcaattcac cttgagactt tccgatacaa tatcgcggaa
      361 tgaccacttg gcgacttccg cgatcacagc ctttttaaat tcagagtcgg tgattttcga
      421 acccgtttcc ctgacttgac tgacttcgcc tgacggcgtg atgtcgagct gcagcaaaat
      481 tacgccggcg aggctcggat ttttggcgat ttcgacgcgg taaagctcac gcacgttgtg
      541 cagttggttt tcgatccgtg tagcgactct gtttttggaa actgcttcat cattggcggt
      601 tatcacggtg aagccgagtt catgggcgaa aaaaaccata ttgtctaacg cgaaattcat
      661 cgcgcgcaaa ggttttacac tttcttgagg ctctgtcgca gttgctcgtg ccgccacttc
      721 tttcttggcc gattcggtgc cgactccgga gctgaagtag acaatggcaa gccctagtaa
      781 acatccgccg attccgacaa tgcccttcat tcccatgccc tttttttcaa cgtgtgttgg
      841 tgtgctgcca gaagatataa tcacctgagc caagcaactt ctatacctca gaaggttcgc
      901 tgtttagcga cacgggcgaa cagtgcagca gcatcgcact cggagagata gcagtgaagt
      961 ttgaggagta ccgcaaagcc ggaatctggc gtgaatgtct ctcgctgtca ctccggacca
     1021 gtgggcgctg gcgcggtagc aatgaggcgg tgaacagttt tcggcgacgt gaaccaattt
     1081 gcgatttctc cacatcttga accggagttg atgagcagtc tagtatgacg ccgcacctgt
     1141 actgtaagca aaaatggagg cgctatgatc gcgattattt tgttgctgtt ggtttttgac
     1201 cgtgccgagt tgttcgcaca gacgccttat tatcagggca agaccattac gttcatcgtt
     1261 ggttcaggcg caggcacggc gtatgacatg tattcgcgcc tgctcgcgaa tcatattggg
     1321 aagcacatcg cgggaaatcc gacggtcgtc gtacaaaaca tgccggcggc gggtggcatg
     1381 gtcgcggcga atttcgtgta cggagtggcc aagcccgacg gtttgaccgt cgtgtcgatc
     1441 aatccggcgc attacttcaa tcagttgcaa ggcaataagg aagtaaaatt cgattggccg
     1501 aaattcacct ggctcgccag ctcggacaag tccgagcaca tgctctacat gcgcgccgat
     1561 gcgccgtata agacgatcca ggacgtgcgc cgcgcggcag agccaccgaa atgcggcgcc
     1621 accgggacgg gaacgagcgg acattatttt cccagaatgc tcgaagagac gctcggcacc
     1681 cgattcaata tcgtgaccgg atacgccggc ggcaatgaga ttgatctcgc gacggagcgc
     1741 aatgaagtgg tctgccgggc attcaccacg gccgcctatt tcgcgcgtga accgtatcat
     1801 acgtggcgca agaaaaattt tgtccgtgtg cttttgcaaa cgggacacaa aagagatcct
     1861 caactgcccg atgtgccgct cctcagcgac ctcatggacg aattcaaaac ttcggaactc
     1921 gggaggcgcg tcgctactgt catgttgggc tctggtgagc tcggtaggcc aatcgtgacc
     1981 tcgccgggac tggcgcctga ccgcgtcaaa atattacgcg atgcattcat gaagagcatg
     2041 gccgatccgg cgttacttac ggacgcggcg aaaaacaagc tcgaaattac gcccgtcagc
     2101 ggcgacgaac tcgcaaagat cgctagagat gtgattgatc agccgacgga agtcgtggag
     2161 cgaatcaaaa aagtcctcgc gcaataaaga gcatcagctt cgataacgcc gatcaatctt
     2221 gcaatcagcc gaattgcaac gtaccgtgaa cgtagctctg attttggagg atttgatgcg
     2281 tccgattcga gttattttcg ttgcgtgcgc ggtcgcaaca ttttttttca acgccgagct
     2341 ttttgcccaa agcgcggcaa agccggcgac cgtcatccaa gcaggttctc tggtcgcatt
     2401 tgattacacg ttgacggatg acaccggcaa ggtcatcgac tcgagccagg gcaaggagcc
     2461 gatgcactac gttcatggca aaggacaaat cattccgggc ctggaaaagg aactgacggg
     2521 aatgtccgca ggcagtgaaa agaaagttac ggtaaaaccg gaagacgcct atggacaggt
     2581 gaacccccag gcgttccaag aagttcccaa agacaagctt ccacctgagg ctctgaaagt
     2641 gggcactgtg gtaaccgcgc gtagccccga gggccaaagc gtcccggtgc gcgtgcacga
     2701 gatcaaagaa aacaccgtcg tgatggactt caatcatccg cttgccggaa agacgctgtt
     2761 cttcgatgtc aaagttaccg aagtaaaacc ggcgaacccc tagtctcttg cctgacttgc
     2821 atcagtccca ggcctggtga agctggttcc cgcgtaaccg gtcttgtcat tcaaatcggc
     2881 tccgtcgcat gttgatctgc ttcagcgttc attattagat tgcgccggtc gcgcagttga
     2941 gtgcgaccga gctgaggcga tgacgtgacg cttttgctta cacgttcgga agtcgcgacg
     3001 ttacttgacc tgcagcgagc aatgctcgtg ctcgagcaga cgtttcgcga gcaatccgcg
     3061 gggcaagtga aaccggtggc gccgctacgg ttcatgaacc gcggcatgcg catggtcgtg
     3121 ggcggactcg aggcgcagga caaaaatggc tttcgtgtga gcgttaccgg cggtgacagc
     3181 ctggcgatgc ttttcgagat cagctcgggg aaacttctct gcttgacggg ttatccgttc
     3241 agcaatctgc gtatcgatca aagggccaca cgtcgatttt cgcatcgcgc cggagcttta
     3301 tccggcgata ctggaaaaat tcaaccggcg agaatattac tggggacccg atccgatgaa
     3361 aattccctgg catgaacagg gcggacatac ggtttacggc tatgatccat ttggcaatcg
     3421 aattcagatt ggcgatcggt ttgggcatta gtcggcgtac tggcgtgttg cctggttttt
     3481 aaaggttgaa ggagggggca atctcagacg agggaaaaca tttatgattc cacaactatc
     3541 catcgaagag cgtgaccgtc gttacaaagt cgtccgtgcc gaaatggcgc agcgcaatct
     3601 cgatgtgctg ttgttgccgg cgaacaccgg tcgatgggag cagttgcagg gagattcgcg
     3661 ttacctgact tcgattggtg gttttgccac cgaggtgttt acggtgttcc cacgacaggg
     3721 cgatgtcact gcttacattt tcaaccgcgc cggttggtgg aagcaggcac agaagtgggt
     3781 ttcggacgtg cgcgacggcc gcaaccggtg gggacaaaat gccatcgagc ggatcagcga
     3841 attgggcctg caaaaaggca ccataggtat ttccgggctg gccggattgt ttcgcgcgcc
     3901 ggagggaatc attccctatt cgtcagtcaa agccattcag gatgcttttc cttcggcgaa
     3961 ggtcgtcaat gccaccgaga tgatgcaagc aataagagca gtgaagagcg ccgaggaggt
     4021 ttcattgctc gaacgttccg cggccatcgt cgaaaaaact attgaagcaa tggtcgaagg
     4081 cgccaacccc ggcgtcagcg aaaaagagct ttatggcgcc atggtgcatg ccatgctcgc
     4141 caacggcggc gagttgccaa cgttattttt cttgagcgga ggcccgggaa tcagcaatag
     4201 ctcgttcgtg ccgacggatt accgactgca aaaaggcgat cgtctgatcg atgagatcga
     4261 ggcgaaatat gcaggctacg ctgctcaggc ggtctgcccg gttgtcctgg gaaaacctga
     4321 cgacgagtat cagacgatga tcgatatctc tcgcgcgtgc ttcgacgcga ttctgcacca
     4381 gatgaagccc ggcgtcactt tcggactgct gtttgaggtt tatcgcgata cggtggagcg
     4441 tctcggcaag gggaaatatc tttggaacca tccggtgatg cacgcgcggg gactcggtga
     4501 cgatggcccg gctttgctcg gcgacagtga tctcgaacgg ttctcgaaaa tcgaactaca
     4561 ggcaggcatg acatttattt tgaagccgca ggtttgtccc aaagaaggta aaggccgcgc
     4621 cagcattggc gacacggtgg cggtcactga aaacagcgcc cggcggttgg gaaaacggga
     4681 gctgaagctg atcgtgaaga atgtttgact ggcaagaccg caacacggaa gacgtagggg
     4741 tgcaatttat tgcgccggat cgcaagggga atcgcccgca acaaacgggc gcgatgaatc
     4801 gcgcccccgc cggactggta cggtgatatg cctgagcttt atccgtaccc acgtttctcc
     4861 acctctgaac gcgatcgccg ctggcaggct gtgcgcgcgc tcatgcgcgc gcagaatctc
     4921 gacgtcatcg tcacgccgca aaacagcggt cattcggcgg attatcaagc caacacacgt
     4981 tatctgactc actgcggtgg cggcgagccg gaccttgcag cggtttttcc gctcgaaggc
     5041 gaggtgaccg caattgcgac ttcagcggcg ccgcgctggc cgacggtgca ggattgggtg
     5101 tttgacgtgc gcgaagctcg gcgcaattac gggcgcgcca ttgtggaacg tttgaaagag
     5161 ttgaaactcg aacgcggccg catcggcatc accggtctcg gcgaagtgga gggcacgcgc
     5221 acgccggaag gcacgatttt ctacggtacg tggaagcaga tccgcgaagc gtttccccat
     5281 gccgagttga tcgacgccac cgcaatcctc gacgaggtgc gttacg
//
LOCUS       DASULZ010000639         3885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1493188, whole genome shotgun sequence.
ACCESSION   DASULZ010000639 DASULZ010000000
VERSION     DASULZ010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493188"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VFY96_13190"
     CDS             complement(<1..302)
                     /locus_tag="VFY96_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607471.1"
                     /translation="MRQLSASLFLWIILCFPTTSGFCADTPPKNARAAQTPVEHHHPA
                     NWRFTLPKGDPVKGRAVFVKYQCYDCHEVRGEDFSEPVEVTAPELSQMGPEHPIEF"
     gene            complement(963..1568)
                     /locus_tag="VFY96_13195"
     CDS             complement(963..1568)
                     /locus_tag="VFY96_13195"
                     /inference="COORDINATES: protein motif:HMM:NF017534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5607472.1"
                     /translation="MPLDKSSDCTHPDFWTMRYGAGKTPWDFGGVPAALKLFLARSSA
                     PGRVLIPGCGSGYELQAFHDARYDVIGLDFSPAAVDHAKKMLGDLGERVTLGDFFTCD
                     FGERRFDLIYERTFLCSMTPSRWPQYVQRIEDLLVTGGSLIGIFLYGQSSSSGPPFPL
                     TNAQAEQLFEKRFQLIRSDTVSDSLPLFRGMERWQEWLKTK"
     gene            1729..1944
                     /locus_tag="VFY96_13200"
     CDS             1729..1944
                     /locus_tag="VFY96_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607473.1"
                     /translation="MPRDRISSRQVRLRENLSSLDLRFIHIIIANRRVFTVCTLAIDA
                     LAKLMIAKGNITDEEFKARLSANYLAL"
     gene            complement(1999..3342)
                     /locus_tag="VFY96_13205"
     CDS             complement(1999..3342)
                     /locus_tag="VFY96_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HEX5607474.1"
                     /translation="MLSNMVDPEGDTMKMFRLVGIAIAVATLFTGQSLAADPAAVNWS
                     GVPTKTVTLFYPGQSTYQWIRSPAHPGAPVVNNGGACLTCHNGAEEKLGSKLVAANPL
                     EPTPVAGKNGVIQLNLQIAYDNANAYFRAQWRTRNLYPGEAHQFVRFDGKEWKTYGFP
                     KLDAVVQKGEQPGLYEDRFSIMVDDGGVPAFANHGCWLTCHDGERDNPKFANRAQVDA
                     NPLYQALKQSDVRKYLPSTRTDENASWDKGKSPEEIAKIKAGGGFLDLMQWRAHRSNP
                     VGMADDGYVLEYRLSDAGGNPFGSNVDPKTHQPRFMYDEKKVGKKALRAEDIRKMPTA
                     LIREQNAVPFDPNARWKEGDLIPEYVLSRETPKGSAADNKNVNGVWKDGMWTVVWVRP
                     LNLTNPDDKALTEGKVYNFSLAVHDDNITTRGHHVSFPVTVGFGAKAVMEATKLK"
     gene            3724..>3885
                     /locus_tag="VFY96_13210"
     CDS             3724..>3885
                     /locus_tag="VFY96_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5607475.1"
                     /translation="MEEPVVAVQPAPRTLPRAIWALGLGAMFMDMSSELVHSLLPIFM
                     VSVLGASMIT"
BASE COUNT          898 a         1027 c         1006 g          954 t
ORIGIN      
        1 aaaaattcga ttggatgttc cggtcccatt tgactcagct cgggtgctgt cacctccaca
       61 ggctcagaaa aatcttcacc gcgcacctca tgacagtcgt agcattgata cttgacgaag
      121 accgcgcggc ctttaacggg atcgcctttc ggtagcgtga accgccaatt tgccggatgg
      181 tgatgttcga caggagtctg cgctgcgcgt gcattctttg gcggcgtatc ggcgcaaaaa
      241 cccgatgtgg tcggaaagca gaggataatc catagaaaaa gtgatgcgct tagttggcgc
      301 atactcatgc tcccttaagt cgacgagagg ccaacagcta ctcatcggct cgacccctta
      361 tctggacaat ttagttgaag aggacaaaag cgacgcgagc gcggcgctag atgcgaatta
      421 cagagagaaa aagatgacgt ggaagagtct ttgaataaag cgggccggga gggccgttgc
      481 cctcctccag tgtaattgat cgtgtcaaat cgttgatcgt cgctgtgtca tggaaagcat
      541 ggtgcattcg agcactgccg gacgaaaggg ataggggcga gctgattaag aactcgctca
      601 aaacatttgg acaatgaatc ttcaatgggt cggtgtgcga atggtgcgcc ggtgcgggat
      661 tatcatgctc gtgggaagat tgttcgctgt gatgagcccc gtgcacctcg gcctcgaaat
      721 caacgcagcc ttccagagcc caaacagctc cggcaaaaaa tatcgagatg caactgagaa
      781 ctaagatcca gcgcttcatc ggagtcgaat ttactctgcc tctgttccct cgcctttgca
      841 acccgagttt aatcaccaag tgaggagtct tagaatgcgg acgcgataat ggcctcgggc
      901 gctccgcgtc gactgaggtt catcgcgatg tcggcctgag gacttttgca cggctccatg
      961 ctctacttcg tctttagcca ttcctgccat ctttccatcc cacgaaatag aggcaaggaa
     1021 tccgacaccg tgtcactgcg tattagctga aaccgtttct caaaaagctg ctcggcctgc
     1081 gcgtttgtaa gaggaaacgg cggtcctgac gacgatgatt ggccatacaa aaaaataccg
     1141 atcaagctcc ctcccgttac caaaagatct tctattcttt ggacatattg aggccagcgg
     1201 gatggcgtca tcgaacaaag aaacgtccgt tcgtaaatga gatcgaagcg gcgttcgccg
     1261 aaatcgcacg taaaaaagtc acccaaggtg acccgctcac ctaaatcgcc caacatcttt
     1321 tttgcgtgat cgaccgcagc cggcgaaaaa tcaagtccga taacgtcata cctggcatca
     1381 tgaaacgcct ggagctcgta accagagcca cagccgggaa tgagtacccg acctggggcc
     1441 gaagatctcg cgagaaacaa ttttaacgcc gccggaactc cgccgaagtc ccatggcgtc
     1501 ttaccggctc cgtagcgcat tgtccagaaa tccggatggg tacaatctga cgatttgtcc
     1561 aggggcatgt cgtctcacta aggatctttc gaatggcgct ttgccatttg cgaaaacaat
     1621 tcggcaccac gcttgtaaag cagatacttt ctttgctgga ttttgcccga tttacttata
     1681 cgaaagcgac aaccggctat gttgaaattc ggaccttggg agcacgaaat gccccgtgat
     1741 cgaatcagca gccgacaagt acggttgcga gaaaacctat cttcgctcga tctccgcttc
     1801 atccatatta taatcgccaa tcgacgggtg tttacggtgt gcacgcttgc gatagacgcc
     1861 ttggctaagt tgatgatcgc caaggggaac atcacggatg aagagttcaa agcccgattg
     1921 agtgcgaatt atctagcctt gtgaagcggt tctactgaac tgtacggccc ttggagtgat
     1981 tagccactcc gtacgtagct attttagctt ggtcgcttcc attaccgcct tcgcgccgaa
     2041 accaacggtg acggggaagg acacgtgatg tcctcgcgtg gtgatattgt catcatggac
     2101 cgctaaggag aagttgtaaa cttttccttc cgtcaaagct ttgtcgtcgg gattggtgag
     2161 gtttagggga cgtacccata ctaccgtcca cataccgtcc ttccatacgc cattgacgtt
     2221 tttgttatcg gcggccgatc ccttaggggt ttccctgctg agcacgtact ctgggatgag
     2281 atcgccctcc ttccatcggg cattgggatc aaacggtacc gcgttctgct cacggatcag
     2341 tgccgtcggc atcttgcgga tgtcttcggc ccgaagagct tttttcccga ccttcttctc
     2401 gtcatacatg aatctcggct gatgcgtttt tgggtccacg tttgatccga acgggttacc
     2461 gccggcatca ctcaaacgat attcgagcac gtagccgtca tccgccatgc cgaccggatt
     2521 tgagcggtgg gcgcgccact gcatcaagtc gagaaagcct ccgcccgctt tgattttggc
     2581 gatctcctcc ggactcttgc ccttgtccca ggatgcgttc tcgtccgtgc gcgtcgaggg
     2641 caggtattta cgcacgtcgc tctgtttcag cgcctggtag agcggattcg catcaacttg
     2701 ggcccggttc gcaaacttcg gattgtcgcg ctcgccgtca tggcatgtaa gccaacaacc
     2761 gtggtttgca aaagccggta ctcccccatc gtctaccata atggaaaagc gatcttcgta
     2821 gagccccggc tgctcaccct tttggacaac agcatccagc tttggaaaac cgtacgtctt
     2881 ccattccttg ccatcgaagc gcacaaactg atgcgcttcg cctggataaa gatttctggt
     2941 tctccactgc gcgcggaaat acgcattcgc attgtcataa gcgatttgga gattgagctg
     3001 aatgacgccg tttttaccag cgactggcgt gggttccaac gggtttgctg cgaccagctt
     3061 gctgccaagt ttttcttcag caccattgtg gcaggtcaga cacgcacccc cattgttaac
     3121 caccggggct ccgggatgag cggggctacg tatccattgg tacgtggact gccccggata
     3181 gaagagagtc acagtcttag taggaacacc gctccaattc acagctgcag gatcggcggc
     3241 cagagactgt cccgtgaata acgttgctac ggcgattgcg attcctacta atctgaacat
     3301 tttcatggta tctccctccg ggtctaccat attactcagc aacccccgaa catttgtgcc
     3361 ggaatctagt ccaaacgcct atggctgtaa agcgataaaa ttgtgccgag aggattggaa
     3421 atagcagttc gcttcccaat cgaatatctt ggtagcgcat catcgccaaa atcatcgttc
     3481 gtgcacagca gattgttttc ggccaatgac tgagtgaaaa ttgcggcacc ggaataaatt
     3541 ttaagatttt gccctcgccc ttttctctta gcctttggaa gctgccacct tggctggttg
     3601 ttgtgttgac agcggtggga ggagaggtga tagctcgcat atagagagta tccatgtgcc
     3661 acagaactac gagagagata aaattataag gaatcgcagt cgttggttgg tcagaagctt
     3721 acgttggaag aacctgtcgt cgctgttcaa ccggctccgc gtactctgcc tcgcgctatt
     3781 tgggcgcttg gacttggcgc gatgtttatg gacatgtctt ctgagctggt acacagtcta
     3841 ttgccgatct ttatggtctc agtcttaggt gccagcatga tcacc
//
LOCUS       DASULZ010000640         4726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_73775, whole genome shotgun sequence.
ACCESSION   DASULZ010000640 DASULZ010000000
VERSION     DASULZ010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73775"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VFY96_13215"
     CDS             <1..279
                     /locus_tag="VFY96_13215"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5607476.1"
                     /translation="GGQLTVGTDLKKDGAAPEIIISVADTGLGIEPSALDKIFQPFYT
                     AKKRRGLGLGLPICERIVQNHGGAMHVQSQPGIGTIFEIRLPAGTPAQ"
     gene            641..1402
                     /locus_tag="VFY96_13220"
     CDS             641..1402
                     /locus_tag="VFY96_13220"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX5607477.1"
                     /translation="MANAEAELIVGIGASAGGLESLKEFFSAIPADCGLAFVVIQHLA
                     PSRASYMADILQKCTKMRVIEAQDNLPIEPNSVYTIPPNRFLKIAKERLYLAEPEKRD
                     GLRMPIDFFFRSLAENQHERTICLLLSGSGSDGTLGLREVRAAGGLTIVQKPETAQFD
                     AMLRGAIATGMVDLVLPIGEIAEVVRRYVPQIRRPDSSSADREKLEQLDSILDLLTLR
                     SGNDFSAYKKPLCCGVPNGAGDSITWARWPSTCNL"
     gene            1491..2156
                     /locus_tag="VFY96_13225"
     CDS             1491..2156
                     /locus_tag="VFY96_13225"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX5607478.1"
                     /translation="MSKHSSETAAKKHPGQSPIRVWIPACSTGEEAYSVAMLFLEEMS
                     AANRSIPLQIFASEIDSEALNFAREGIYPHSIANDASEARLSRFFTKHDSHYQVIKSL
                     RESVIFSVHNLLSDAPFSRLDLISCRNFLMYLEPVAQRRLIALFAFALNRNGHLFLGK
                     SDAIVSQDSFFVPVFENGESIAAALPQLPAYRNLRTAAKVARGVWTRAIDDRIPAPGT
                     YPN"
     gene            2153..2413
                     /locus_tag="VFY96_13230"
     CDS             2153..2413
                     /locus_tag="VFY96_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607479.1"
                     /translation="MIQEALFERFKTSVLLVDEHGTIRYFFGPTAKYLEHPTGEANNN
                     LLSMIDASSGARIRIGLRRAIEEQTPVDLQPVEITHRARAHP"
     gene            2436..2666
                     /locus_tag="VFY96_13235"
     CDS             2436..2666
                     /locus_tag="VFY96_13235"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX5607480.1"
                     /translation="MRQHNRALEQQLIASGRLVSLGEITASMAHEFNNPLGVIMGFVE
                     DLLEETDPSSPQYQSLRIIDEESKRCEKLLRT"
     gene            2666..3556
                     /locus_tag="VFY96_13240"
     CDS             2666..3556
                     /locus_tag="VFY96_13240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5607481.1"
                     /translation="MQFARPGNAQPRQTYIHAVIDTTLHMMESRLYKEKVTLARRIQP
                     DLPPIEADPQQLEQVLVNLYLNAIDAMPEGGTLTVCAAVERAGEEPVIALTVTDTGRG
                     MSADEVKKIFQPFYTANKKTGLGVGLPICERIIKNHRGRIEVQSEPGQGTTFKIILPV
                     TAARGDHPPRIASNPIRIQPNISGGTYKALREGGTFFSGWRRLAAHYSYIPELQRDYE
                     IRATDAEKSADSIRDLLNGGKDPQCKTNPLQNNRHSTPFCLRYDTVICRSDRNKAAAA
                     ISEQTIFGITAISRLIPTLQ"
     gene            complement(3537..4241)
                     /locus_tag="VFY96_13245"
     CDS             complement(3537..4241)
                     /locus_tag="VFY96_13245"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009630230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX5607482.1"
                     /translation="MVKAAIFDIDGTIIDSVGAHIDAWKQTFAHFGKQVSDEEIRRHI
                     GEGSDDMLPVFFSSEELHRFRRELESYRGELFKREYLPSLKAFPDVRPLFERIKNDGK
                     KIALGSTAKAEEINIYKEIARIADLVDCAVSSEEVVQSKPHSDICGVALRKLGKLSPA
                     KVIAIGDTTYDVEAAARNNVCTIGLLCGGGTREELQRAGCVALYHDPADLLAHMVGCF
                     WARRRWVDQPSTVMSG"
     gene            complement(4297..>4726)
                     /locus_tag="VFY96_13250"
     CDS             complement(4297..>4726)
                     /locus_tag="VFY96_13250"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HEX5607483.1"
                     /translation="FGFQLIAVFNQGFEEKLSTVQQQLHLVSIILTICAVGLVMTPAA
                     LHRQIDPLAVTRRFIEWSTMLLLLSMFPLGISISLELYLIAQIITGSFWLSLLAASGV
                     LVLFFALWVLLPNLKRGGGRRTMRHHLGITKVAEESDHRR"
BASE COUNT         1092 a         1387 c         1253 g          994 t
ORIGIN      
        1 ggcggccaac tcacggtcgg caccgattta aaaaaagatg gcgcggcgcc ggaaatcatc
       61 atctcggtag ccgacaccgg actcggcatc gaaccctcgg cgctcgataa aattttccag
      121 cccttctaca cggcgaaaaa acgccgcggc ctgggtttgg gactgccgat ctgtgagcgc
      181 atcgtccaga accacggcgg cgccatgcac gttcagagcc agcccggtat cgggactatt
      241 tttgagattc gcttgcccgc cgggacgcca gctcagtaaa gccgtctttc ccgctcgaag
      301 agaccagcga aaaccagcac cgtcacgaag ctgaattcgc cacttgcatt ttttctgcac
      361 ccaagcctaa tttggagcca atcactaacg tttgagctga gctgcggaac cgcacacgga
      421 tcccgaatct aaagcacggg aggatccttc cgataccgag tggatctgtt taaatcttgg
      481 ccgatagcag taagccttga caaccatttc cacaactcgc tagttctcga tcgaaaccgg
      541 ttcgccaggc attacctgcc gcggagtcaa aagttaccca gtgccacaat agcgattagg
      601 aaaatagctt gctctattct aggttacctg tcctgcgtca atggctaacg cagaagcaga
      661 attgatcgtc ggcatcggcg cctccgccgg aggcctcgag tcgctgaagg aattcttcag
      721 cgccattccg gccgattgcg gcctggcgtt cgtcgtcatc cagcatctcg ctccatcgcg
      781 ggcgagttac atggctgaca ttctccagaa atgcaccaag atgcgggtca tcgaagcgca
      841 ggacaacctg cccatcgagc ccaactcggt gtatacgatt ccgccgaatc gatttctaaa
      901 gatcgcaaaa gaacggcttt acctcgccga acccgagaag cgcgacggcc tacgcatgcc
      961 gatcgacttt ttctttcgtt cgcttgctga aaatcaacac gagcgcacga tttgtctgct
     1021 gctttccggc agcggttccg acggaacctt gggactgcgc gaggtgcgcg ctgccggcgg
     1081 cctcacgatc gtgcaaaaac ccgaaaccgc acaattcgac gcgatgctgc gcggcgccat
     1141 cgccaccggc atggtcgatc tggtattgcc catcggtgag atcgctgaag tcgttcgccg
     1201 ctacgtaccg cagatccgcc ggccggacag ctcgagcgcc gaccgtgaaa aactcgaaca
     1261 gctcgacagc attctcgatc tgctcacttt gcgcagcggt aatgattttt ccgcctataa
     1321 aaaaccactt tgctgcggcg taccgaacgg cgcaggggac tcaatcacct gggcacgttg
     1381 gccgagtacg tgcaatctct gatcagcaac cctaccgagg tcgacgcctt ggccaaagac
     1441 atgctgatcg gggtcagcag cttcttccgt gatccggaag cattcgagga attagcaaac
     1501 acagtagtga aaccgctgct aaaaagcatc ccgggcagag tccgatacgc gtctggatcc
     1561 ccgcctgttc gacgggtgag gaagcctatt cggtggcgat gctcttcctg gaggagatgt
     1621 cggcggccaa tcggagtatt ccactacaaa tattcgcttc cgagatagac tccgaagcat
     1681 tgaattttgc ccgcgaaggg atctacccgc acagcatcgc caatgacgct tcggaagcgc
     1741 gcttgagccg cttcttcacc aaacatgact ctcattacca ggtgattaag tcgctgcgcg
     1801 aatcggtcat tttttccgtg cacaatctgc tcagcgatgc gccgttttcc cggctcgacc
     1861 tgattagctg ccggaatttt cttatgtacc tcgagcccgt ggcccagcga cggctgattg
     1921 cgcttttcgc tttcgcgctg aaccgaaacg gacacttgtt tctcggcaaa tccgacgcca
     1981 tcgtttcaca ggacagcttt tttgtacccg tatttgaaaa cggcgaatct atcgccgcag
     2041 cacttccaca gctcccggcc taccggaact tgcgtaccgc cgcaaaagtc gcgcgtggag
     2101 tttggacgcg ggcaatagac gatcgaattc cggctccggg tacctatccc aattgattca
     2161 agaggcgctc tttgagcggt tcaagaccag tgtactgctt gtggacgagc acggcacgat
     2221 ccgctatttc ttcggaccta ccgcgaaata cctagagcat ccgaccggcg aggccaataa
     2281 caatctgctg agcatgatcg acgccagcag tggcgcgaga atcagaattg gcttgcgccg
     2341 cgcgatcgag gaacaaacgc cggtcgactt gcagcccgtc gagatcactc accgagcgcg
     2401 agcgcatccg tgagctgatc gccagcgagg agcaaatgcg ccagcacaat cgggcgctcg
     2461 agcagcagtt gatcgcctcg ggccggctgg tatcgctggg cgaaattacc gcgtccatgg
     2521 cgcacgagtt caacaatccc ctcggcgtga ttatgggatt tgtcgaagat cttctcgaag
     2581 agaccgatcc ctcgagtccg caatatcagt cgttgcgaat cattgacgag gaaagcaagc
     2641 gctgcgaaaa attattgagg acctgatgca atttgcccgt ccgggaaacg cgcagccgcg
     2701 ccagacctat attcatgcgg tgatcgatac gactttgcac atgatggaga gccggctcta
     2761 caaggaaaaa gtaaccttgg cgcgccgcat ccaacccgat ctgccgccca tcgaggccga
     2821 tccgcagcaa ctcgagcaag tactggtcaa tctctatttg aatgccatcg acgccatgcc
     2881 ggagggcggc acgttgaccg tgtgcgctgc cgtcgagcgc gctggcgagg agccggtgat
     2941 tgcgcttacg gtcacggata ccggccgcgg aatgagcgcc gacgaggtga agaaaatctt
     3001 tcagcctttc tatactgcca acaagaaaac cggactcggt gtaggattac ccatctgcga
     3061 acgcatcatc aaaaatcatc gcggccgaat tgaagttcag agcgagccgg gacaaggcac
     3121 gactttcaaa atcattctgc ccgtcaccgc tgcccggggg gatcaccctc cccgcatagc
     3181 cagtaacccc attcggattc agccgaatat ttccggcggg acttacaaag ctctgcggga
     3241 aggtggtact tttttctcag gctggcggcg gctggcggcg cactatagct atatcccgga
     3301 gctgcagcgc gattatgaga tacgcgccac cgatgccgag aaaagcgccg actcgatccg
     3361 cgatttgctc aacggtggaa aggatcctca gtgcaagact aatccgcttc agaacaaccg
     3421 gcactccact ccgttttgtt tgcggtacga tacggtcatt tgtcgatcgg atcgaaataa
     3481 agccgcagcc gcaatctccg aacagacgat tttcggtatc accgcgatct ccaggcttat
     3541 cccgacatta cagtagaggg ttgatcaacc caccgccgac gagcccagaa gcagccaacc
     3601 atatgggcga gcaggtcggc cggatcgtga tacagcgcga cacagccggc ccgttgcagt
     3661 tcttcgcggg tgccgccgcc acaaagcagc ccaatcgtgc aaacattgtt tcgcgctgct
     3721 gcctcgacgt cgtatgtcgt atctcctatc gcgatgactt ttgcagggga tagttttccg
     3781 agcttccgga gcgcgacacc gcagatatcg ctgtgcggtt tcgactggac aacttcttcc
     3841 gaggagactg cgcagtccac cagatcagcg atacgagcga tttctttgta gatgttaatt
     3901 tcctctgctt ttgccgttga gccgagggca attttcttgc cgtcgttttt tatccgttcg
     3961 aatagcggcc ggacgtcagg gaaggctttc aagctcggaa gatattcgcg tttaaagagc
     4021 tcgcctcgat agctttccaa ctcccgacgg aagcggtgta actcctctga agagaaaaat
     4081 accggtaaca tgtcgtcgct accctcgccg atatggcgcc ggatctcctc gtcggagacc
     4141 tgcttgccga aatgggcgaa ggtctgcttc caggcgtcaa tgtgtgcacc cacagagtcg
     4201 atgatcgtcc cgtcaatgtc gaagatagct gctttaacca tttttaattc cccatccacg
     4261 cgccgcctcg tcatcgaaag acttcacccg caacccctac cggcgatgat cagactcctc
     4321 agccactttg gtgataccca agtgatgccg cattgtgcgc cgtccgccgc ctctcttgag
     4381 gttcggcagc aagacccaca aggcaaaaaa caaaaccaag actccgctgg cagccaatag
     4441 gctaagccag aaagatccgg tgatgatctg ggcgatgaga tagagttcca aagatatgct
     4501 tatgccgagg gggaacatgc tcaggagcaa cagcattgtg ctccattcta tgaatcgtcg
     4561 ggtcactgct agcggatcga tttggcgatg gagagcggcc ggagtcatca cgagaccgac
     4621 ggcacaaatc gtcagaataa tcgacacgag atgaagctgc tgctggacgg tcgacaattt
     4681 ctcctcgaat ccctggttaa acacggctat tagctgaaag ccaaat
//
LOCUS       DASULZ010000641         3638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_174297, whole genome shotgun sequence.
ACCESSION   DASULZ010000641 DASULZ010000000
VERSION     DASULZ010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174297"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VFY96_13255"
     CDS             <1..433
                     /locus_tag="VFY96_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607484.1"
                     /translation="FPNTDYIIRREHLQTHRTEVVSFMKGILEGMRAIKANRALGIHA
                     IQKYLKMNSAEEAGIAYDYYVGQKWDRFPRFHRVQRCLPASNRRSIKGRRYTGKSEAH
                     RSVHFGGNHQERFRDSALQIKNIAGKITARTIDFSSDVLNT"
     gene            484..1434
                     /locus_tag="VFY96_13260"
     CDS             484..1434
                     /locus_tag="VFY96_13260"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5607485.1"
                     /translation="MAEDPTTVRGSGGNPPSRVKGPLVWLDMDQKELDDAYDQSVYAP
                     NQQLISTRRRLASEAMLKRVTPERIAYGSSEDEKLDIYKTTRANAPINIFVHGGAWRN
                     GQASDSAYLAESFINAGAYFVIPDFVLVHNAGGSLMAMVQQVRRAVAWVYKNAAQFGG
                     DPDRLYITGHSSGAHLAGCALVTDWPKEFGLPAHILKGGLLASGMYDLKPVRLSKRSE
                     YVKFTDEIEQALSSQRHLDKLTAPIIVAYGTQETPEFQRQNREFAAAVKAAGKPVELI
                     VGEGFNHFELQETIANPYGILGRAALKMMNLSMSPGKEQT"
     gene            1431..1970
                     /locus_tag="VFY96_13265"
     CDS             1431..1970
                     /locus_tag="VFY96_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607486.1"
                     /translation="MSRLPAAKREDLTPEHQQIWDRIHATRSGGGGPYSMLMHSPAMA
                     QHFAATEDYFRTQSLLPDSDREVIILAAARELEARYAWCRHEVRAHNVGIPPELIEAL
                     RAKAPISEFTGRDRLLVEFTRALVHDHTVSDELFTRVEREYGRAKFLEAVGLLGHYMT
                     IGTVIRLFQVEPPEGTKTF"
     gene            complement(2189..2317)
                     /locus_tag="VFY96_13270"
     CDS             complement(2189..2317)
                     /locus_tag="VFY96_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607487.1"
                     /translation="MNYTVLAHVMARVPNASFHDATDVIGLARYVKSEEEISFIRE"
     gene            complement(2703..3164)
                     /locus_tag="VFY96_13275"
     CDS             complement(2703..3164)
                     /locus_tag="VFY96_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEX5607488.1"
                     /translation="MQATTRRRRIAVRNSPIHGQGVFALRRIPKGTRIIEYKGTLITD
                     EEADQRYSRIQAHSPHTMLFSVDDGLVIDATRKGNSARWINHSCAPNCEIEEEHHRVF
                     IEARRDIAPGEELTYDYNLQIGEKHTKSAKREHACFCGSRRCRGTMLGEEE"
     gene            complement(3194..>3638)
                     /locus_tag="VFY96_13280"
     CDS             complement(3194..>3638)
                     /locus_tag="VFY96_13280"
                     /inference="COORDINATES: protein motif:HMM:NF015214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5607489.1"
                     /translation="DIEILRSCIQVAEAKGYFSPGGTWAPRLSAAYRVHSIEASDRAE
                     RTMEGLLTSTLMLSGGASDLNSGVGPLVERYFRGGAPSTKEHLRLMAIAADMVMTPFG
                     KRSQLYERLQSGDVDRMRQRLYGQYQDPVPAERMREFISRMDRER"
BASE COUNT          803 a         1081 c          993 g          761 t
ORIGIN      
        1 gtttcccaac accgactaca tcatccggcg cgagcatcta cagacacacc gcactgaagt
       61 cgtgagtttc atgaagggga ttctcgaggg catgcgagcg attaaagcga atcgagcgct
      121 cggcatccac gcgattcaga aatatttgaa gatgaacagt gccgaagaag cagggatcgc
      181 ctatgactat tacgtcggtc aaaaatggga cagattcccg aggttccatc gcgtgcagcg
      241 ttgcttgccg gcatcgaaca gacgctcgat aaaaggacgg cgttacaccg gaaagtctga
      301 agctcaccga tcggtccatt ttggaggaaa tcatcaagag cggtttcgtg acagcgcttt
      361 acaaataaaa aatattgccg ggaaaatcac ggcgcggaca attgactttt cttccgacgt
      421 tttgaacact tgaacgattt caacgaactg aacttctcga aaagaagccc aacgggaaaa
      481 tatatggctg aagatccaac gacggttcga ggctccggcg gaaatccgcc gtcccgagta
      541 aaaggccccc tcgtctggct cgatatggac caaaaggagc tcgacgacgc atacgaccag
      601 agcgtttatg ctccgaatca acagctgatc tcgacgcgtc ggaggcttgc cagtgaagca
      661 atgctcaagc gggtaacgcc cgagcgcatc gcctacggct cctccgagga tgaaaagctc
      721 gatatctata agacgacccg cgccaatgcg ccgataaata tttttgttca cggcggcgcc
      781 tggcgcaatg gacaagcgag cgactccgct tatctggccg agagcttcat caacgccggc
      841 gcttactttg tcataccgga ctttgtcctc gtccataatg ccggcggcag tctaatggcg
      901 atggttcagc aggttcggcg cgctgtcgcc tgggtctata aaaatgccgc gcaattcggc
      961 ggcgatcctg atcggctgta catcaccggc cactcttccg gcgctcacct cgctggctgt
     1021 gcgctcgtaa ccgattggcc caaggaattc gggctaccgg cgcacattct taaaggcgga
     1081 ctgttggcca gcggcatgta cgatttgaaa ccggttcgtc tttccaaacg gagcgagtac
     1141 gtgaaattca ctgacgagat cgagcaggcg ctcagctcac aacggcactt ggacaaactc
     1201 accgcgccga tcatcgtcgc ctatgggacg caagaaacgc ccgagtttca gagacagaac
     1261 cgcgaatttg ccgcggcggt caaagccgcc ggtaaaccgg tcgagctaat cgtcggggag
     1321 gggttcaacc atttcgagct gcaggaaacc atcgccaatc cttacggcat cctcggccgc
     1381 gccgcgctga aaatgatgaa cctgtcgatg agtccgggta aggagcaaac atgagccgac
     1441 tgccagctgc aaaacgagag gatctcacgc ccgagcacca gcagatttgg gatcgcattc
     1501 acgctacccg cagcggagga ggcggcccct actcaatgct catgcattcg cccgcgatgg
     1561 cgcagcattt tgcggcgacc gaagactatt tccgcactca atcgctgctg ccagacagcg
     1621 accgtgaagt catcatcttg gccgcagccc gtgagctcga ggcgcgctat gcgtggtgcc
     1681 gccacgaggt ccgcgcgcac aatgtcggaa ttccgccgga actcatcgaa gccctgcgcg
     1741 cgaaggcgcc gatttccgag tttaccggcc gagaccggct gctcgtcgag tttacccgtg
     1801 ccctcgtgca cgatcatacg gtttcggacg aattgttcac gcgcgtggaa agggagtatg
     1861 gccgcgccaa atttctcgaa gccgtcggac ttctcggcca ctatatgacg atcggcacgg
     1921 tgatccgact gttccaggtc gagcctccgg aaggaaccaa aactttctag ttagttacaa
     1981 accgagcagg tgccgtgaac agctgaagga tgcatgaatg atttctcgct ccgctcgaaa
     2041 taacagagaa agagtactct gtcatcccgg agggatgtga gggatctttc ttcgtgtact
     2101 gccggtctca actactggtc ggatacgggt tcgccatctc cgcgaagaat ccttctttct
     2161 cctcgaggcc cgcgctcgcg actcgggcct attcgcgaat aaaagaaatc tcctcttcac
     2221 ttttcacata acgcgccaac ccgatcacgt cggtcgcatc atgaaaactc gcgttcggca
     2281 ctctggccat gacgtgtgcg agcacggtgt agttcacgac cccgtcgatc gaacggcccg
     2341 ggcacccact cgttgcccga atcttgattg gatatgacaa ttgcctcgcg gcggtccctg
     2401 gggagaatga ttgcagcgtt gtcgagctga gtcaggaatc gacaatcgga gcgcgttccg
     2461 cgcgcctgtt cgagagacag atgcagattg cgtccgtccc acgcacagcg gcactaagat
     2521 gcagtcgagt cccgccttcg cggcgttatc ccggacgcgt tgccagcgcg agtcccgctc
     2581 ggcaagggaa tagccgcccc attcaatttc cgccgatgct tccatcgcgc agccgttccg
     2641 tttcgatcta ttgcgttcat catagacgac gggtcggtcg ccgctgatgg gctaaagttg
     2701 ccctattctt cttctcccaa catcgtgccg cgacagcgac ggctgccgca aaagcaagca
     2761 tgctcccgct tggccgactt ggtatgcttc tctcctattt gcagattgta atcgtaggta
     2821 agctcttccc ccggcgcgat atcacgtcgc gcctcgatga aaactctatg atgctcctcc
     2881 tcgatctcgc aattcggcgc gcaagaatga ttgatccagc gcgccgagtt tcctttgcgg
     2941 gtcgcgtcga tgaccaagcc gtcatcgacg gagaaaagca ttgtgtgagg cgaatgcgcc
     3001 tggattcggc tgtagcgctg atccgcctcc tcatcagtga tcaaggtccc tttgtactcg
     3061 atgatgcgcg ttcccttcgg gatccgcctt agagcaaaga cgccctgtcc gtgtatgggc
     3121 gaattgcgca cggcaatgcg acggcgcctg gtcgttgctt gcatgactac tcccaaacga
     3181 tcagcgatgg ctctcagcgc tcgcgatcca tcctgctaat gaattcgcgc atgcgctctg
     3241 ccggcactgg atcctggtat tggccgtaca gacgctggcg catgcggtcg acgtcaccgc
     3301 tttgcagacg ctcatagagt tggctgcgtt tgccaaacgg ggtcatcacc atgtcggcgg
     3361 cgatcgccat caagcgcagg tgttcctttg tgcttggcgc gccgccgcga aagtaacgct
     3421 cgaccagagg accgacgccg ctgttgagat ccgatgcccc gccgctcaac attaacgtac
     3481 tggtcagaag tccttccatc gtccgttccg cccgatccga agcctcaatg ctatgcaccc
     3541 gataggcagc gctcaatcgc ggtgcccagg taccgcccgg actgaaatac cctttcgcct
     3601 cggcgacctg aatacaagaa cgaaggatct caatatct
//
LOCUS       DASULZ010000642         4969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_73811, whole genome shotgun sequence.
ACCESSION   DASULZ010000642 DASULZ010000000
VERSION     DASULZ010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73811"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VFY96_13285"
     CDS             complement(<1..478)
                     /locus_tag="VFY96_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX5607490.1"
                     /translation="MAIGLSHGGTTIYSSPSLSNEVLIGTCEGVVTIARAASASEWKV
                     AHRAITDKHISAIIKEPESGLTFAGAFHGSIHVSKDDGKTWETRANGMTQPNVYSLAA
                     KRIDGRVRVFAGAEPAHLFVTDDLGLNWKEIPSLRSVPSVPRWSFPAPPHIGHVKHI"
     gene            complement(542..1552)
                     /locus_tag="VFY96_13290"
     CDS             complement(542..1552)
                     /locus_tag="VFY96_13290"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607491.1"
                     /translation="MISNTPLHGAEQALREPPLKLRLGLGSAPAPPLPNSVLWLAKDL
                     GFYAREGLDVELNEFQGTPLAIAAMLSGDVDIANISTSEVIRMNAAKGQPMRAIHSPD
                     ARLYFMIVARDELKSVTALQGRTFAVSRLGSVDHSLSSIALKSLGLNPASMTMIAMGV
                     PSTRAQALVAGRVDATSLSIGTWITIQKEPGVRILVDHNTYFENATVVEKVNAVTSKV
                     IDEKPEQLRRFTAAIVKASRHFAESQDAWVAAMTKRRPDVDRSDAANLWNGFKTSWAV
                     NGLMNLDAYKRSAEFFYQTGILDKVPRIEVAEWADTRFVDGVLKEVGISPKADPVGRS
                     VK"
     gene            complement(1805..2569)
                     /locus_tag="VFY96_13295"
     CDS             complement(1805..2569)
                     /locus_tag="VFY96_13295"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX5607492.1"
                     /translation="MIEFNSRVDKIATDLQEQIRRGKLKPGERLPSERSLCSVFGVGR
                     TTIREALKSLTVLGLVTRQGRGAVITDPELISATTADLAGLAAQTSIRQLFELRKLIE
                     VKVAGWAALRAVPEDIQSMKRAIEAERMRDTAAGNPNRMFHDALVKAAHNPALIQVYE
                     SGRNIFFRLPFYWKLFDDAEVKNVRAARHELARRWHEQILNAIEDRDAAEAEGAMFQH
                     LDVMEKDLLNRLQGNSSGDNSSYSHPVLANFAVISK"
     gene            2676..3644
                     /locus_tag="VFY96_13300"
     CDS             2676..3644
                     /locus_tag="VFY96_13300"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607493.1"
                     /translation="MDSKEKIVVFGPALPMNLAPMWVAHDKGFFKEEGLDVDLQPVQG
                     IPDSQHPRHQWRKDGTIIFQSPGGSPPFRSVLENRDHIDGEVNVVSIANRTAHVFVAK
                     PYVKDVVDLKGRKVAGDLKGGSSMDAKMVMRHFGIDPEKDVTWIDSRGKPPNTERFRL
                     ALFEKGEVDAVCCDPPHWNIAVQMGGHALKSCRDLFSIPEAGFSTSPAVIAEKPLVVK
                     GMVRALLRGVIVAKYSKEETLDSILRHNHFINRELASIAYDEVHKEWGPVLDLDAYQR
                     KVDFYTNEWNLPKKPVRDYYNFKYLKEALDELDMLRSWDPRMDFKE"
     gene            3641..4669
                     /locus_tag="VFY96_13305"
     CDS             3641..4669
                     /locus_tag="VFY96_13305"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5607494.1"
                     /translation="MKRIALCLLFMFGFAPELRAEPPFFEDKTMRLVLGFSPGGISDL
                     WARALARAMTPHIPGKPSIISQNMPGAGSLTAANYIYNIAKPDGLTIGFVTPGLYFNQ
                     LTDMKEVRFDWAKFSWIGSPERTVRIIYGRSDAPYKTLDEMRNGAEQPRCGATALGTV
                     GHYFPRLIEEVAGVKFSIVTGYPGAAEIDLAMQKGEVQCRAGSLEGYFGSEPTRTWSK
                     SGFTRVLVHGSPKRDPRLPEVPTVAEILDKRKAPDVTRRLAVVLMAPDSIGRPLIAPP
                     GLPADRLATLREALMKALDDPEFKAEAQKRNWEVEPVTGQELEAIAKKVVVQPPDVVE
                     RMKKVLAN"
BASE COUNT         1109 a         1363 c         1426 g         1071 t
ORIGIN      
        1 tgatgtgctt tacatggccg atgtgcggcg gggcaggaaa gctccatctg ggcacgcttg
       61 ggaccgaacg aagcgagggt atttctttcc agttgagccc gaggtcgtcg gtgacaaaca
      121 gatgcgccgg ttcggcgccg gcgaacacgc gcacgcgacc atcgatgcgt tttgccgcta
      181 gcgaatagac gttgggctgg gtcatgccgt tggcacgtgt ttcccacgtc ttgccgtcat
      241 ctttactgac atgaatcgag ccatgaaagg cccctgcgaa ggtcagaccg ctttcaggtt
      301 ctttgatgat tgcgctgatg tgcttgtcgg tgatcgcccg atgcgcgact ttccattctg
      361 atgccgaagc cgcgcgggcg atggtcacga cgccttcgca ggtgccgatc aaaacttcgt
      421 tcgacaaaga gggagaggaa taaatcgttg taccgccgtg cgacaatccg atcgccatag
      481 agctaccctc ctaaaataat ttgaacaaaa ttcccgattg acgtgatgcg agctcatcct
      541 ttcattttac agaacggccc acgggatcgg ccttggggct gatgccgact tccttcagca
      601 cgccgtcaac aaatcgcgta tcggcccatt ccgcgacttc gatccttgga accttgtcca
      661 aaattcccgt ctggtaaaaa aactctgcgc ttctcttgta ggcatcgaga ttcatcaggc
      721 cgttgaccgc ccaagacgtt ttaaagccgt tccagagatt agccgcgtcg cttcggtcca
      781 cgtctggccg gcgctttgtc atggcggcaa cccaagcgtc ctggctttcc gcaaagtgcc
      841 ggctggcctt gacaatggcc gcagtaaacc gacgcagttg ctcgggcttc tcatcgataa
      901 ctttactcgt aaccgcattc actttttcca ccacggtcgc gttctcaaaa taagtattgt
      961 ggtcgaccag aattcgcacg cccggctctt tctggatcgt aatccaggtg ccgatcgaaa
     1021 gcgaggtcgc atccacccgg cccgctacca acgcctgagc gcgggtggaa ggcacgccca
     1081 tcgcgatcat cgtcatgctc gcgggattaa gtcccagcga tttcagcgca attgaagaga
     1141 gcgagtgatc cacgctaccg aggcgtgaca ccgcgaacgt tctaccttgg agagccgtga
     1201 cggatttgag ttcgtctctc gcaacgatca taaaatagag ccgggcgtcg ggcgaatgaa
     1261 tcgcgcgcat cggttgtccc tttgcagcgt tcatcctgat gacttccgaa gtcgaaatat
     1321 ttgcgatgtc gacgtccccc gaaagcatcg cagcgatcgc cagcggcgtg ccttgaaatt
     1381 cgttcagctc gacatcgagc ccctcgcggg catagaaccc caagtccttc gccagccaaa
     1441 ggaccgagtt cggcaggggt ggagcgggag cggagccgag ccccagccga agcttgagag
     1501 ggggctctcg taaagcctgc tcggcgccgt ggagtggcgt gttcgaaatc aaatagagcg
     1561 cgacaaaaaa cgttccgatc gcggctctga actttctgaa attatgtcgt gttgagattc
     1621 ctccctttgg caaagggagg ttaggaggga ttttgtttcg ggagagcaaa tccccctcga
     1681 tccgcttttc cgaaagtggg aaatccgggt gcaggtcaga attcttaaac aaagtggtgg
     1741 gccagcagac ttggaaaatt ttcatggttt cgttcagtac tcgacgtcgg atcacaagcc
     1801 ttgcttattt gctgatgact gcaaaatttg ccaacacggg atgcgagtac gatgaattat
     1861 cgccgctgct gttgccctgc aagcgattga gaagatcttt ttccatgacg tccaggtgct
     1921 gaaacatggc gccttccgcc tcggccgcgt cgcggtcctc gatcgcgttc aatatctgtt
     1981 cgtgccaacg gcgggcaagt tcatggcgcg ctgcgcgcac gtttttgact tccgcgtcgt
     2041 cgaagagttt ccaataaaaa ggcagacgaa aaaaaatatt tctgcccgac tcgtagacct
     2101 gtatgagcgc gggattgtgg gcggctttga ccaacgcgtc gtggaacatg cggttgggat
     2161 tacccgcggc agtgtcacgc atccgttcgg cctcgatcgc ccgtttcatg gattgaatat
     2221 cttcaggcac ggctcgcagc gccgcccatc ctgcgacttt gacttcgatc aatttgcgca
     2281 gttcgaagag ctggcggatt gaggtctgcg cggcaagtcc ggccaaatcg gcggttgtcg
     2341 cagaaataag ctctggatcc gtgatgaccg cgccgcggcc ttgcctggtg accagcccaa
     2401 ggacggtcaa agacttcaag gcttcgcgaa tcgtggtgcg tccgacgccg aataccgaac
     2461 agaggctccg ctcggagggc agtcgttcgc ccggcttcaa tttaccgcgt ctgatctgct
     2521 cctgaaggtc ggtggcgatc ttgtcgacac ggctgttaaa ctcaatcatg gtcagaccaa
     2581 aatctccttg acacaccaaa ttcgtctgct gtatagcaaa actcgtcaac tttggccaga
     2641 ccaatttctg gtattcagtg aggaggcttc ggcagatgga ttcaaaagaa aagatcgttg
     2701 tattcggccc ggcgctgcca atgaatctcg cacctatgtg ggtcgcccat gacaaaggtt
     2761 ttttcaaaga ggaagggctg gacgtcgacc tccagccggt ccaaggcatc cccgacagcc
     2821 agcatccgcg ccatcagtgg cgcaaggacg gcacgattat ttttcaatcg cccggtggct
     2881 cgccgccgtt ccgctcggtc ttggagaatc gcgatcatat cgacggcgag gtcaatgtcg
     2941 tctcgatcgc caaccggaca gcccatgttt tcgttgccaa accgtacgtc aaagacgtgg
     3001 tagatctaaa aggcagaaag gtcgccggcg atctaaaagg cggctcgagt atggatgcaa
     3061 agatggtgat gcgccatttc ggcatcgatc cggaaaagga cgtcacctgg atcgacagcc
     3121 gcggcaaacc gccaaacacc gaacggtttc ggttggcgct gttcgaaaag ggcgaagtcg
     3181 acgcggtgtg ctgtgacccg ccccactgga acatcgcggt acagatggga ggtcatgcgt
     3241 tgaagtcctg tcgtgatctt ttttcgattc ccgaagcggg tttttccaca tcgcccgccg
     3301 tcatcgcgga gaagccgctc gtggtcaaag gcatggtgcg cgcgcttcta cgaggggtca
     3361 tcgtcgccaa gtacagcaag gaagagactc tcgattcgat tctgcgccac aaccatttca
     3421 tcaatcgcga gcttgcgagc attgcttacg atgaggtgca caaggagtgg ggaccggtgc
     3481 tcgacctaga cgcctaccaa cggaaagttg atttttatac caacgagtgg aacctaccga
     3541 aaaaaccggt gcgcgattat tacaatttca aatatttgaa agaagcgctc gacgagctcg
     3601 atatgctgcg ctcctgggat ccgcggatgg atttcaagga atgaagcgca tcgcattgtg
     3661 cttgcttttc atgttcggtt tcgcgcctga gctacgcgcg gagccgccgt tttttgaaga
     3721 caagacgatg cgcttggtgc tcggcttttc gcccggtggc attagcgatt tgtgggcgcg
     3781 cgctctggcc cgcgccatga cgccgcatat acctggcaag ccaagtatca tctcacaaaa
     3841 tatgccgggc gccggatcgc tgaccgccgc aaactacatt tacaacatcg ccaaacccga
     3901 cggcctgacc atcggatttg tcaccccggg gctttacttc aatcaattga cggatatgaa
     3961 ggaggtcaga tttgattggg cgaagttcag ctggatcggc tcgcccgaac gcacagtgcg
     4021 gatcatctat ggccgcagcg acgcgccgta caagacattg gacgagatgc gcaatggcgc
     4081 cgaacagccg cgctgcggcg cgacggcgct gggtacggtg gggcattact tcccgagact
     4141 gatcgaggaa gtcgccggcg tgaaattcag catcgtcacc ggatatcccg gcgcagcgga
     4201 gatcgacctg gcgatgcaaa agggcgaagt gcagtgccgc gccggcagcc tggaaggata
     4261 tttcggcagc gagccgacgc gcacatggtc aaagtccggc ttcacgcgcg tgttggtgca
     4321 tggcagtcca aagcgagatc cgcgcttgcc tgaggtgcca accgtcgccg aaattttgga
     4381 caaacgaaaa gcaccggacg tgacccgccg cctggcagtg gtgctgatgg cgcccgactc
     4441 catcggtcgg ccgctgatcg cgccgccagg tttgccggcg gaccggctgg cgacgttacg
     4501 cgaggcgctc atgaaagcgc tcgatgatcc cgagttcaag gcagaagcac aaaagcgaaa
     4561 ctgggaggtc gagccggtca ccgggcaaga gctggaagcg atcgcgaaaa aggttgtcgt
     4621 ccaaccgccg gacgtggtcg aacggatgaa gaaggtattg gcaaattgaa gataagattt
     4681 taaccacaga ggccacggag gacacaacaa gagaaaaatc attgtagagg cgaccggccg
     4741 gtcgcccttg gctggaccga agagaccaaa atttattatg tgacctttgt gtctctgtgg
     4801 ttcagaaccg ccgaggtatt catgtacaag acgactagag aagaaaataa gattctcact
     4861 cgcaccgggc gaggcacacc aatgggtgaa tttttgcgtc gctactggtg gcccatcggt
     4921 atctctgcgc atttgaaaac aaagccgact ttcattcgcg tcttcggag
//
LOCUS       DASULZ010000643         2076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1493571, whole genome shotgun sequence.
ACCESSION   DASULZ010000643 DASULZ010000000
VERSION     DASULZ010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493571"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(320..1372)
                     /locus_tag="VFY96_13310"
     CDS             complement(320..1372)
                     /locus_tag="VFY96_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607495.1"
                     /translation="MKQQAFQSERIEIDDELWAANDFFEAKGWSDGLPIVPPTEDRVA
                     RMLSATKRKADEVIGTVPPKWASATVEKIAINAVMAGCKPEYMPVLIAAVEAITDPKL
                     NLYALQATTGGPAVMLIINGPVRHRIQVNGGYNALGEGWRANATIGRCIRLIQRNIGG
                     SYPETTCKATLGWPGKYSICIGENEEASPWEPLHVERGFDRNSSTVTAISADGSVRAS
                     DLDSTTAEGVLTNFAQKMDGPSGPEAIMVICPEHAKIIANDGFSKKDVQQFVWQRAAY
                     RMKDLPEETFNQRVKRWSHRNLTRESIIPVTDHPEDIVVVVAGGDGSQSQYIHVWGQS
                     TPEGGSTRSVTKAVGE"
     gene            complement(1404..1535)
                     /locus_tag="VFY96_13315"
     CDS             complement(1404..1535)
                     /locus_tag="VFY96_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607496.1"
                     /translation="MQHPIGTVKAEEVKKRADAVFEKLLEALLDARQASNGDSRALS"
     gene            complement(1683..1967)
                     /locus_tag="VFY96_13320"
     CDS             complement(1683..1967)
                     /locus_tag="VFY96_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607497.1"
                     /translation="MIVLSPEGKARTSSERIAALPRFADLNGKTLGLLDNSKPNNDML
                     QARFEQLLRQNCDVAGVVRRRKLTAQQGAPKAYLDDLAAHADFVISGLGD"
BASE COUNT          434 a          584 c          558 g          500 t
ORIGIN      
        1 aagaagagca ggacaaatat gaatggaaaa acaatccttc ttttggcggt gatcataaac
       61 tcgatagctc cccggttcgc aaccacggct gaaggtcgct gcggaattga atgatgccaa
      121 ggctgataat gttgcggctt agcgccagag gtaaaagctg ggcatgactc tgaacaaaca
      181 acaatcggcg tgccagctat ttcacagata tcgttgtacg ttggtcgata tgcataacgc
      241 gaacgacacc tacgaacgcg cggtcaatcg aactaaagtt cagcaacgac cgcgatctgt
      301 agcgttcaca caatctcggc tattcaccga cggccttggt caccgagcgt gtcgagccgc
      361 cttcgggagt gctctgaccc cacacgtgga tgtactgcga ttgagaaccg tctccgccgg
      421 ctacgaccac gacgatgtcc tcagggtgat cggtgacagg aatgatactc tcacgggtaa
      481 gatttcggtg cgaccaacgc ttgactcttt gattaaacgt ttcctcaggc aaatccttca
      541 tgcggtaggc tgctcgctgc caaacaaact gctgcacatc tttcttactg aagccgtcat
      601 tggcaatgat ttttgcgtgc tccgggcaga tcaccatgat cgcttcaggc cccgaaggtc
      661 cgtccatctt ttgagcaaaa ttagtcagca cgccctctgc ggtggtgctg tcgagatcgg
      721 aagcgcgcac ggagccgtcg gcgctgatcg ccgtgaccgt gcttgaattc ctgtcaaaac
      781 cgcgctcgac atgcagcggc tcccacggac tagcctcttc gttctcaccg atgcagatgg
      841 agtacttgcc aggccaacct agggtcgcct tgcaggtcgt ctcagggtac gagccgccga
      901 tgttccgctg gatcagacga atgcaacggc caatggttgc gttggcccgc cagccctcgc
      961 cgagcgcatt gtagccgccg ttgacctgga tccgatgccg cacgggtccg ttgatgatca
     1021 gcatcaccgc cggcccgccg gtcgttgctt gaagtgcata gaggttcaac ttcgggtcgg
     1081 tgatcgcttc cactgcagcg atgaggacgg gcatgtattc gggcttgcaa cccgccatga
     1141 cagcgttgat ggcaattttc tccacggtgg ccgaggccca cttcggcggc actgtgccga
     1201 tgacctcgtc cgctttgcgc ttggtagcgg agagcatccg cgcgacgcga tcttccgtcg
     1261 gcggaacgat gggcagtccg tcactccaac ccttcgcttc aaaaaaatcg ttggcagccc
     1321 agagttcatc gtcaatttca atccgctccg actgaaatgc ttgttgtttc atgttcctta
     1381 ttcccaacgt atgcgaacta gaattacgaa agcgctcgag agtctccatt tgaggcttgg
     1441 cgcgcatcga gtaaggcttc gagcagcttc tcgaagacgg cgtcagcacg ctttttgact
     1501 tcttcggctt tgaccgtgcc gatcggatgc tgcacgatca caatcggcga atcgggagca
     1561 ccgaaagctt cgcgctccga cttcgccagc gccttaaagg gatgagagat aacggtgaca
     1621 gcgagccggc cgcgtttttg tagttcaatg gaatcgtgga tactccacga tgtgcaggac
     1681 cctcaatctc ccaatccgct gatgacgaag tcggcgtgag cggcgagatc gtcgaggtac
     1741 gctttgggcg cgccttgctg ggcggtaagt ttgcgccgcc ttacaacgcc tgccacatcg
     1801 caattctgtc tgagtaactg ctcgaaacgt gcttgaagca tatcgttgtt gggcttgctg
     1861 ttgtccaaca aacccagcgt cttgccgtta agatctgcaa agcgtggcag cgcggcaatc
     1921 cgttccgatg atgttcgcgc ttttccttcc ggacttaata caatcattgt ttctccttgt
     1981 tcggttcgat cgcattcttt gacctattct gagcggattc tgtctcgtcg aaatcgactc
     2041 ttattgggtt tttaccctct gtctaatgcc tatttg
//
LOCUS       DASULZ010000644         3452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_681803, whole genome shotgun sequence.
ACCESSION   DASULZ010000644 DASULZ010000000
VERSION     DASULZ010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681803"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VFY96_13325"
     CDS             <1..565
                     /locus_tag="VFY96_13325"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HEX5607498.1"
                     /translation="DHYVASFYAAFLKLGCRPSVLTFFKRTPDVRSFLLDQDVIYIGG
                     GNTKSLLAVWRDWGVAEILYEAWQSGIVLTGVSAGAICWFEQGLTDSFSDGLRPLACL
                     GFLPGSCCPHYDGESQRRPTLHRLLAAEQISPGIAIEDWTGVHFRDKEIYKAITSKRG
                     ARAYTVRPVYGSVQEVPMPTEYLQGSG"
     gene            732..1949
                     /locus_tag="VFY96_13330"
     CDS             732..1949
                     /locus_tag="VFY96_13330"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEX5607499.1"
                     /translation="MNILVLNCGSSSIKFQLIETAGDLAAITQDRKMIVGLLDRLGED
                     ATFKLKAGDAVTPKEQKIAAPDHEVGVREIIQRLDLTMPGSSGVRRIDAIAHRVVHGG
                     DLFTEAAVIDEKTLARIESLDDLAPLHNPAAVSGIHAARKIFRSTVPMTAVFDTAFHH
                     TIPETAATYAIPYDLAAKHRIRRYGFHGMAHQYSMLRYAALSGVSPERVKLVTLHLGN
                     GCSACAIRDGRSVETSMGFTPLEGLVMGTRSGDLDPALVSFLARKENVDAAEVERWLN
                     QRSGLLGLSGRSNDMRELLSRYENDQRARLAIDVFCYRARKYLGAYLAALGGAQAIVF
                     SGGIGENAPAIRESICREMAWCGLRLDTERNNAAIAVAAAIAAPDARIGVHVIPSDEE
                     AIIAAETACLLSK"
     gene            2030..2503
                     /locus_tag="VFY96_13335"
     CDS             2030..2503
                     /locus_tag="VFY96_13335"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5607500.1"
                     /translation="MRGTSQLKKRRRMPVVGLVMTSFPYFIEVDDDANRMENMMDEHG
                     IRHLPVQENGKVVGIVSERDLHHRVKRDAPAAEKNTILARHIMVADPYIVSFRAPLSD
                     VVLEMARRRIGSVIVQRQGKLAGILSAIDVCRILGEYLESMFPTGRRGGGGGAAA"
     gene            complement(2601..2990)
                     /locus_tag="VFY96_13340"
     CDS             complement(2601..2990)
                     /locus_tag="VFY96_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5607501.1"
                     /translation="MLARDMMTRDVITVTPSMTIKQLANALIKNQVSGAPVTDKKGKI
                     IGIVSEADIVARKGKDVKSIMSKKIVSIDEETSVEEIAHLMMTHHVKRVPVMRGDQLV
                     GIVSRADIVSAIARGEHVAIHTPVYDL"
     gene            complement(3193..>3452)
                     /locus_tag="VFY96_13345"
     CDS             complement(3193..>3452)
                     /locus_tag="VFY96_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019555298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607502.1"
                     /translation="KLEYLDFAALQDSKTGKTRVRAVEIDAPSYKVAREYMIRLEKED
                     FSDDNKLRLLAEAASSTNRPCSMDEFRDRYQYLTADLKGCI"
BASE COUNT          749 a          951 c          985 g          767 t
ORIGIN      
        1 ggaccactac gttgcgagtt tctatgcggc gtttctaaaa ctggggtgtc ggccctcggt
       61 gctgacgttt ttcaaacgca cgccggacgt gcgctctttc ctgctcgacc aggacgtgat
      121 ctatatcggc ggaggcaata ccaaaagctt gctcgcggtg tggcgcgatt ggggtgtggc
      181 ggagattctt tacgaggcat ggcagtctgg tattgttctc accggcgtga gcgcaggtgc
      241 gatctgctgg ttcgagcagg gactaaccga ttcattttcc gacggactgc gcccgctcgc
      301 atgtctgggc tttttgccgg gaagctgttg tccccactac gacggcgaat cccaacgccg
      361 cccgacattg caccggctgc tggcagccga gcaaatttca cctggcattg ccattgagga
      421 ctggaccgga gtgcattttc gagacaagga aatctacaaa gcgattacgt caaaacgagg
      481 agcgcgcgcc tacaccgtac ggcccgtcta tggttcggtg caggaggtgc cgatgccgac
      541 ggaataccta caaggctccg gctagcgaag ctaaagctgg tctttgctgt ttttggttcg
      601 aagcgcctct ctttgcacaa ccgaattagg ctagccaagc gatgcaaggc tcaatgggtc
      661 ggcagggcaa ttgagcgcga ggtgcgcaag gctagctcgc tttcgaagat aattctctta
      721 aaatcgtttc tatgaacatc ctggtactca actgcggcag ctcctccatc aaattccagt
      781 tgatcgaaac tgcaggcgat ttggcggcta taacgcaaga tcgcaagatg atcgtgggcc
      841 tgctcgaccg gctcggcgag gacgctacat tcaagctcaa agctggtgac gcggtcaccc
      901 ctaaagagca gaaaatcgcc gcgcccgacc acgaagtggg ggtccgtgaa atcattcaac
      961 gtctcgatct gacaatgccg ggaagctccg gtgtacgccg gatcgatgcc atcgcgcacc
     1021 gcgtagtcca cggcggtgat ctctttaccg aggcggcggt gatcgatgaa aagacgctcg
     1081 cgcgcatcga gtcactggac gatctcgcac ctctgcacaa tcctgcggcg gtgagcggca
     1141 tccacgcggc gcggaagatt ttcagaagca cagtaccgat gaccgccgtc ttcgatacag
     1201 cttttcacca caccataccc gagactgcgg caacgtatgc aattccctat gatcttgctg
     1261 ccaagcaccg aatccgccgt tacggtttcc acggcatggc gcaccagtat tcgatgctgc
     1321 gatacgcagc cctgagcggt gtttcgccgg agcgggtcaa gctcgtaacc ttacatttgg
     1381 gtaacggttg ttcggcgtgt gcgattcgcg acggccggtc ggtggagaca tcgatgggtt
     1441 ttacaccgct cgaagggctg gtgatgggaa ctcggtcggg cgatcttgat ccggcactgg
     1501 ttagttttct cgcgcgcaag gaaaacgtcg atgccgctga agtcgagcgc tggctcaatc
     1561 agcgctccgg cttgctcggc ttgtccggtc ggtcgaacga catgcgcgag ctgctctccc
     1621 gatatgaaaa cgaccaacgt gcgcgactag cgatcgacgt tttctgttac cgcgcacgca
     1681 agtatttggg agcttatctc gcagcgctcg gtggcgcaca ggcgatcgtc ttcagcggcg
     1741 gcatcggcga aaatgcgccc gcgatcagag aaagtatctg ccgggaaatg gcgtggtgcg
     1801 gtttgcgatt ggacaccgaa cgaaataatg ccgctatcgc ggtcgcagca gccattgccg
     1861 cgccggatgc gcgcatcggt gtccatgtca tcccgagcga cgaggaagcg atcatcgcag
     1921 ctgaaaccgc ctgtttactt tcaaaataaa agcgcgtccc gagacgctct tgacatccgc
     1981 acccgccgcg ggacaagtct gcagttaatc atggcgcatg ggaggagtga tgcgcggaac
     2041 atcgcaacta aaaaaacgac gcaggatgcc ggtggtcggc ctcgtcatga cgtcttttcc
     2101 ttactttatc gaagtcgatg acgatgcgaa tcgaatggaa aacatgatgg atgagcatgg
     2161 catccgtcat ttgccggtgc aggaaaatgg caaagtcgtg ggcattgtat ccgagcgcga
     2221 cttgcatcat cgcgtcaaac gcgacgctcc ggctgccgag aaaaatacta ttttggcgcg
     2281 ccacatcatg gtggccgatc cttacattgt ttcttttcgg gcgccgctca gtgatgtcgt
     2341 tttggagatg gcccgccggc gcatcggctc ggtcattgtc cagcgccaag gaaaacttgc
     2401 gggcattttg tcagccatcg atgtgtgcag gatcctcggt gaatacctgg agtcgatgtt
     2461 tccaacgggc cgcagaggcg gaggcggggg cgccgccgca taaatacgaa tcgaccagcg
     2521 agactgtcgg caatcctagt ttgctgatgt tcgggctcgg tctcaaaccg agggggaacg
     2581 aaagtcccga aaaacccgat ttaaaggtcg taaacgggag tgtgaatggc gacatgctcg
     2641 ccacgcgcaa tggcgctcac gatatcagcc cggctaacaa taccgacgag ctgatcaccg
     2701 cgcatgacgg gaacacgctt gacatgatgg gtcatcatca agtgcgcgat ttcttccacc
     2761 gaagtttcct catcaatgct gacaattttt ttgctcataa tggattttac gtctttgcct
     2821 tttctggcga cgatatcggc ctcggaaacg atgccgatga ttttcccctt cttatcggtg
     2881 acgggcgcgc cgctgacctg atttttaatc aaggcgttcg ccaattgctt gatcgtcatg
     2941 ctcggcgtga cggtaatcac atctctggtc atcatatccc tggcaagcat tggtctcctc
     3001 ctagaatcgc cgattagctg atcgcggcgc gggcgccagt ccaagtcgcg gcgcagaaag
     3061 ctggtgactt atctttgaac cgggctgtga atacatgaaa aaatcgacaa agccaagggc
     3121 catgcagctc ttggcgagct tagtgaccca aaccatttca cacgagctac ggcatgcgcg
     3181 accgccgtcg cgctagatgc aacccttgag atcggcggtc aaatattgat accggtcacg
     3241 aaactcatcc atgctgcaag gccggttcgt cgatgatgcc gcttcggcga gcagtctcaa
     3301 tttattgtcg tccgaaaaat cttccttttc gagccgaatc atatactcgc gagccacttt
     3361 gtaggacggc gcatcaattt ccacggcgcg cactcgagtc ttgccggtct tggaatcttg
     3421 cagcgcagcg aaatccaaat attcgagctt gc
//
LOCUS       DASULZ010000645         2084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_782949, whole genome shotgun sequence.
ACCESSION   DASULZ010000645 DASULZ010000000
VERSION     DASULZ010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782949"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..1492
                     /locus_tag="VFY96_13350"
     CDS             128..1492
                     /locus_tag="VFY96_13350"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX5607503.1"
                     /translation="MATPPPVTDTLAQFVTKTEFAAISEKALANAKMHLLDTLGVALA
                     GTREPVAALALDYCKRIGPAHESTIWGTKSKAAAPTAAFANGLLAHALDYDDWDAFIH
                     VGHPTSMLLGAGLSVGESTGASGKELLKAYVLGIEVMCKLAANCPNVQDRGFHSTPVW
                     GSLGAAVACGSLLRLTAAQVRAALGIAASAAGGIHRQQGSMVKPFHAGNAARNGAEAA
                     LLARSGFTADAAILEAPRGFCDTFFGPATCDYQKMIENIGQPFYLESPALGLKWHPCS
                     APQFLAADAALHLKHEHDINYEDIARIEVRIPPLRYQRHYAAEVKTGLRGKFAINYVV
                     AMCMLDGKLELETFTDHKANQPKVQEALGKVDVVCDETVPEPGPYCPVTVELTSGARH
                     SFTARIAKGDPRNPMTENEVTEKFRGNAQAILSDQQADKVIATVRNLEALESVGDLGL
                     CMVP"
     gene            1661..>2084
                     /locus_tag="VFY96_13355"
     CDS             1661..>2084
                     /locus_tag="VFY96_13355"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5607504.1"
                     /translation="MEDLKPVKSGKVKVDEVNLYYELYGAGDPLVLVAGTGISLAPWR
                     VSQVPEFAKHYQVLIYDHRGLGRSDKPDMHYTTRLFAKDCVGLMNALRIEKAHMMGHS
                     MGARVCQWIALDYPEKVRSLVLSGPGSGKYSEDLEDYPR"
BASE COUNT          472 a          561 c          594 g          457 t
ORIGIN      
        1 ccgtccaaac aacctttcat tcttggtctc ctgatttatt gatcgatctc ttcgctgctg
       61 tctgttgcct ggtgccgtta tcttcagata tggagctttc atatatccca attccaagga
      121 gcacaagatg gccactcctc cgccggtcac cgacacactg gcgcagttcg tgacgaagac
      181 cgaattcgcc gcgatttccg aaaaagcgct tgccaatgcg aaaatgcatc tcctcgacac
      241 tttgggcgtg gcgctcgccg gcacgcgcga gcccgttgcc gcgctcgcct tggattactg
      301 caagcgaatc ggcccggcgc atgagtcgac catctggggc acaaaatcaa aagccgccgc
      361 gccaaccgcg gctttcgcga acggactact tgcgcatgcg ctggattacg acgactggga
      421 tgcatttatc catgtcggcc atccaacaag tatgttgctc ggtgcggggt tgagcgttgg
      481 cgaaagcacg ggcgcttccg ggaaggagtt gctgaaagct tacgtgctcg gcatcgaagt
      541 catgtgcaaa cttgccgcca attgtccgaa cgtccaagat cgtggctttc acagcacgcc
      601 ggtgtggggc agccttggcg ctgccgtcgc gtgcggtagc cttttaaggc tgactgcggc
      661 gcaagtcaga gcggcgctcg gcatcgcggc gtcagccgcc ggtggcatac atcgccagca
      721 aggatcgatg gtgaaaccgt tccatgccgg taacgccgcg cgaaacggcg ctgaagcggc
      781 gctcctcgct cgatccggtt ttacagccga tgccgcgatt ctcgaagcgc cgcgtgggtt
      841 ttgtgatacg tttttcggcc cggcgacatg cgattatcaa aaaatgatcg agaatatcgg
      901 ccaaccattc tatttggaat ctcctgcctt gggacttaaa tggcatccgt gcagcgcgcc
      961 gcagtttttg gccgcggatg cggcgttgca cttaaaacat gagcacgaca ttaattatga
     1021 ggacatcgcc aggatcgaag tcaggattcc gccacttcgg tatcagcgcc actatgccgc
     1081 ggaagtaaag accggactgc gcggcaagtt tgccatcaat tacgtcgtcg ccatgtgtat
     1141 gttggacggc aaactcgagc ttgagacctt cacggatcac aaagcgaacc agcccaaagt
     1201 gcaggaagcg cttgggaaag tggatgtcgt ttgcgacgaa accgtacctg aaccgggacc
     1261 gtattgtccg gtgaccgttg agttgacgag cggcgcgcgg cacagtttca cagcgcgcat
     1321 cgccaagggc gatccgcgca acccaatgac tgaaaacgaa gtgactgaaa aatttcgcgg
     1381 caacgctcag gcgatcctgt cggaccagca agcagataaa gtgattgcga ccgtgcggaa
     1441 tctggaagca ctcgagagcg tgggagacct gggtttatgc atggtcccgt agctctggaa
     1501 gtaagaatag gttcctctct cgctcgaatg acaactgaat acagcaccgt catacagaat
     1561 catgcgacag atctttcttc tgttgaatct ggagcctatg ttgacacgat ttttccaatt
     1621 cgatagcgtc accagcattc tcgctgcgta agacccaact atggaagatc tgaagccggt
     1681 aaaaagcggc aaagtcaaag tcgacgaggt gaatctctat tacgaactct atggcgccgg
     1741 cgatcctctg gttctggtgg ccggcaccgg tatcagtctg gcaccctggc gggtttcgca
     1801 agtgccggaa tttgcgaagc actatcaggt gctgatttac gatcatcgtg gtttggggcg
     1861 aagcgacaag cccgacatgc attacacgac gcggctcttt gccaaagatt gcgtcggttt
     1921 gatgaatgcg ctgcggattg aaaaagcgca tatgatgggg cattccatgg gcgcacgagt
     1981 atgtcagtgg atcgcattgg attatccgga aaaagtacgg agtctggtgc tttccggccc
     2041 cggctcggga aaatacagcg aagatctcga agattatccg cgcg
//
LOCUS       DASULZ010000646        10980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_275920, whole genome shotgun sequence.
ACCESSION   DASULZ010000646 DASULZ010000000
VERSION     DASULZ010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10980
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275920"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..681)
                     /locus_tag="VFY96_13360"
     CDS             complement(16..681)
                     /locus_tag="VFY96_13360"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEX5607505.1"
                     /translation="MMAPLDDGATKPKTRWTDSLRSHFLAGLLVFLPLAITLWFIGWV
                     INLLDGVLAVLPDALHPNSYLPFAIPKLGAVVTLLLILFLGFLTRGVATRRFLGAWEK
                     VFVQIPVFRGIYTAAQKLVQTFLGQSNAQRQVVMIEYPRSGVFTIGFAMGRAWSHLED
                     KKNAQLVNVFIPTTPNPTSGFYLLVPTDEVTPLNLTMEEGLKLITSSGLITPDDRARI
                     NGK"
     gene            complement(687..1532)
                     /gene="panC"
                     /locus_tag="VFY96_13365"
     CDS             complement(687..1532)
                     /gene="panC"
                     /locus_tag="VFY96_13365"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX5607506.1"
                     /translation="MQIIETVSAMRDWSEREWRSGRRIAVVPTMGFLHEGHLSLVRDA
                     KKHADRVVVSIFVNPTQFGPGEDFAAYPRDFERDCRLLEKEQIDIVFHPALGAMYPDG
                     AETYVEVEKLSLPLCGAMRPGHFRGVATAVAKLFNMIRPDVAIFGKKDYQQLQVIRRM
                     VRDLSMEVEIVGHPIVREPDGLAMSSRNAYLTPDERRAALCLCRSLCKAERLVRRGET
                     SAQSLLSFVRAELAQEPLANLEYVKLCDPETLIDIETLDRSALLALAVRFGKARLIDN
                     RVLTR"
     gene            complement(1596..2399)
                     /gene="panB"
                     /locus_tag="VFY96_13370"
     CDS             complement(1596..2399)
                     /gene="panB"
                     /locus_tag="VFY96_13370"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX5607507.1"
                     /translation="MGEKITAPHIVKMKQRGEKITCLTAYDYAFARILDDAGVEMLLV
                     GDSLGCVIQGNHNTLAVTMDEMIYHTRLVARGRKRALVIGDMPFLSYQVSKEKALENA
                     GRFLQEGGAEAVKLEGGVIMHETIEALVKVGIPVMGHIGLTPQSVHQFGGYKVQGRQK
                     ERREAVLHDALAVEDAGAFSLVLEGMPVDLAEEITRRLTIPTIGIGAGIGCDGQVLVI
                     HDMLGLFDDFQPKFVKRYADLKSIMLGAVKSYISDVKEKKFPTEEHSFK"
     gene            complement(2515..3156)
                     /locus_tag="VFY96_13375"
     CDS             complement(2515..3156)
                     /locus_tag="VFY96_13375"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEX5607508.1"
                     /translation="MGNAKYIVVEGPIGVGKTSLAKILANEFQARTVFERVEDNPFLP
                     KFYKSRETYAFQNQTFFLLNRYQQQVELAQQDLFSQNTVADYLFAKDQIFASLTLSAE
                     ELNLYQQIYALLNARVPKPDLVVYLQARSEVLYKRIKKRDKKYERGVTFEYLGEVAQA
                     YNRFFFHYDETPLLVVNTSEIDFVSSSKDLADLIKEINSMGSGTQHYIPLGSR"
     gene            3143..4294
                     /gene="nagZ"
                     /locus_tag="VFY96_13380"
     CDS             3143..4294
                     /gene="nagZ"
                     /locus_tag="VFY96_13380"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEX5607509.1"
                     /translation="MALPIGTRIYTLPERLSRLWYIVDVQWLRQKLGQMLMVGCHGET
                     LTPDEELVFEEYAFGGFILFRQNCRSPEQISDLCRSLWNTAAEEPPFLAIDQEGGRVH
                     RLPEPFTHFPSAAIIGQSGNPEVAYQCGRATAAELTIAGLNLDFAPVLDVNSNPANPI
                     IGDRAFGRTPKQVIEMSSAWMRGLRDGGIIPCGKHFPGHGDTERDSHLELPVVEKSLD
                     QLRTIELPSFVNACRAGIEALMTAHVRFSALDRELPATLSEAVVTGMLRHQFGYDGLI
                     FSDDLEMKAISANFPAAEAAELAVRAGVDVLLFCDELNKAIDVFESLCALAENDPELR
                     ARIETSFRRITGLKRRILTAFTGLSGGESLARLEAMNHQRIVDRIYGSL"
     gene            complement(4279..5622)
                     /locus_tag="VFY96_13385"
     CDS             complement(4279..5622)
                     /locus_tag="VFY96_13385"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX5607510.1"
                     /translation="MSVVEKNYELEAYFHDAGKPRDKWRVGTEYEKVGIYRDTGQAIP
                     YFGKRGVAFILRELIERFGWDAEEQDGNIIALTRDKAQITLEPGGQIELSGEPCDSIH
                     CTYAEFNQHIRELLEVAEPLGIIFLGLGMQPVSRLDQIEWVPKKRYRIMAPYMLKVGK
                     LGQRMMKQTATVQANIDYNDEKDAIAKFRTGMGLAPVLIGMFANSPICDGEINGYRSF
                     REHIWTDTDRNRSGLLKFAFAPEASFAHYVEYALDAPMYFIIRNKNYIDMTHMTFRQF
                     LEDGYNGERATIEDWGDHLTTLFPETRIKRYIEIRSVDSQPPDLMPALSALVKGAFYD
                     SDCLQAAWDLVKGWSWDERMQAYLDSHRDALATRVRRYALLDLARELLEIAWEGLRRQ
                     NQVNALGEDETIYLKPLKDLLAQGKCPADLLLEKWEGELHHDIKRLIAYTAYKLP"
     gene            complement(5609..6640)
                     /gene="mtnA"
                     /locus_tag="VFY96_13390"
     CDS             complement(5609..6640)
                     /gene="mtnA"
                     /locus_tag="VFY96_13390"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX5607511.1"
                     /translation="MTVKTIEWKNDRVVMLDQRLLPHREVYRVCRDYQQVASAIRDMV
                     IRGAPAIGVAAAMGVALGALKAPPKTFDREFERILSTLAKTRPTAVNLFWALQRMRQI
                     YSDKRSQGIDVVKRALKEEAQKIYHQDIAANKQLGKFGASLLRNTKKIMTHCNAGALA
                     TAGYGTALGVLRALKDSGKEFEVFVNETRPFLQGARLTAWELCKEKIPATLITDSMAG
                     YLMQRGKIDAVVVGCDRVAANGDVANKIGTYTIAVLARRHAIPFYVAGPTASIDINCA
                     TGKDIPIEERDVKEVSHIFGKALAPKGIRILNPAFDVTSHDLITAIITEKGVIHPPYL
                     QNIRTHVCR"
     gene            6706..6984
                     /locus_tag="VFY96_13395"
     CDS             6706..6984
                     /locus_tag="VFY96_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607512.1"
                     /translation="MDFYRLLGYFSGLLFVKPEMDVSALWRTAALVHFLDAILCCVIA
                     KYSGRSKKLWTVAGAICGIWALAVIFLLPEKNDLKAPPRSEEQTKTFK"
     gene            complement(7088..7489)
                     /gene="acpS"
                     /locus_tag="VFY96_13400"
     CDS             complement(7088..7489)
                     /gene="acpS"
                     /locus_tag="VFY96_13400"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX5607513.1"
                     /translation="MIVSTGVDLAEVNRIQAALEDPRIGARFRERVFTPEEIEYCEKK
                     QRGKYESYAGRFAAKEAVMKALGRGWGAKVRWLDIEVARARSGKPAIVLHDKTARLAQ
                     ELGIHRWALSITHTKDHGLAFVVAEDDSGEK"
     gene            complement(7486..7824)
                     /locus_tag="VFY96_13405"
     CDS             complement(7486..7824)
                     /locus_tag="VFY96_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011317911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5607514.1"
                     /translation="MKPKETSCPAEYTLRLIHGRWKIPLIFHLLNGAKRFSELARALR
                     GVTQKMLTQQLREMERDGLVERKIFAEVPPRVEYSLTPLGTSLKPVVDSMCRWGLAHG
                     ASPSAREAQA"
     gene            7924..8967
                     /locus_tag="VFY96_13410"
     CDS             7924..8967
                     /locus_tag="VFY96_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5607515.1"
                     /translation="MRTAVLKKVLDHYPTGKATPNPPLASDRDAALLDAYSQAVVYAA
                     EKVSPSVAFIEVSKALPQRRSTVERRPEARGSGSGFVFTPDGFILTNSHVVQGASKIA
                     VTLSDGRRFDASLVGHDSGTDVAVIRISAPALVPAPLGDSEKIRVGQLAIAIGNPFGF
                     QYSVTAGVVSALGRSLRSESGRLIDSVIQTDAALNPGNSGGPLVNSNGEVIGINTAVI
                     LPAQGLCFAVAVNTAKFIAGHLIMHGRIRRAYLGVGGQNVAIPRFVVRAQQLQTESGV
                     LVISSDKESPAAQAGLKDGDVIVGLGDSPVRSVDDLHKLLTEARIGSRCELTLLRQSQ
                     KISLTVIAQEAPA"
     gene            9051..10271
                     /locus_tag="VFY96_13415"
     CDS             9051..10271
                     /locus_tag="VFY96_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5607516.1"
                     /translation="MDHSKIKGVTHVLKRFSLTLLLIIIFGLAGTAAAQQKGRIKIAV
                     QAPLSGEQAAIGEHIKLGAQLAVEESNKAFKDLGYDLILVPQDDQAKPEVGVANARNM
                     VADPEVLVIVGHFNSGVALPSSEVYKDAMLTMISPANTATEITDRGYPNVNRVCGRDD
                     VQGPVGARFTAQDLKLKSVYIIHDKTLYGQGVAENFRNEAKKLGLTVLGFDGTEERAN
                     FSPMIIPLKAKNPDLVYFGGIYHQGGLLLKQLREKGVKAKFMGPDGLDSAEMAKIAGS
                     AVVGSYYTSVAGPPDAYPETAAFAKKYKQRFGKETESFGMYGYDATLVGIKAIEGWIK
                     ANPGKKPSRADVVTAVRNIKNFKGVTGSIEFDNKGDPVKAKYFVLQFEKQAYPGKVVK
                     VIEQQAPAAVARKS"
     gene            10401..>10980
                     /locus_tag="VFY96_13420"
     CDS             10401..>10980
                     /locus_tag="VFY96_13420"
                     /inference="COORDINATES: protein motif:HMM:NF013942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase A"
                     /protein_id="HEX5607517.1"
                     /translation="MRVALLFGGKSAEHEISLVSARNIAKAMDKGKYDLVAIGIDRQG
                     RWHLDEGARLLFDERRTTVEFTERKTLAAVRPGENSTPLLREGGAGIGVIDVVFPVLH
                     GPFGEDGTIQGLLKLADLPFVGAGVLGSAVGMDKDVMKRLLRDAKIPTPKFVVFEKRE
                     KKSIQFKTIKNILGLPFFVKPANLGSSIGISKV"
BASE COUNT         2389 a         3146 c         3001 g         2444 t
ORIGIN      
        1 tccgaggtcg gctgcttatt tgccattgat ccttgcccga tcatcagggg tgatcaaacc
       61 gctcgaggtg atcaacttca aaccttcttc catggtcaaa ttgagcggcg tgacttcatc
      121 ggtcggcacc agcagataga agccggatgt gggattgggc gtggtcggaa taaaaacatt
      181 gaccaactgc gcattctttt tgtcttccaa atgggaccat gcccgcccca tggcaaaacc
      241 gatggtaaac actccgctgc ggggatattc gatcatgacc acctggcgct gtgcattgga
      301 ctgaccgagg aaggtctgca ccagtttctg tgctgcagtg taaatcccac gaaacaccgg
      361 gatttgaaca aaaacctttt cccaggcacc gagaaatcgc cgagtcgcca cgcctcgggt
      421 cagaaagccg agaaaaagaa tcagcagcag tgtgacgacg gcgccgagct tgggaatcgc
      481 aaacggcaga tacgaattgg gatgaagcgc gtccggcagc accgccaaca cgccgtctaa
      541 caagttgatc acccagccga taaaccagag ggtaatcgcc agaggaagaa acaccagcaa
      601 tccggcgaga aaatggctcc gcaggctgtc ggtccagcgc gttttcggtt ttgtcgctcc
      661 gtcgtcgaga ggcgccatca ttcgctttag cgggtgagca cccggttatc gatgagacgc
      721 gcttttccga agcggaccgc caacgcaagg agcgcgcttc gatcgagcgt ttctatgtcg
      781 atcaatgttt cagggtcgca gagtttaacg tattccagat tcgcgagcgg ctcctgcgcc
      841 aactcagcgc gtacgaacga gagcaatgac tgagctgagg tttcgccgcg cctgaccaac
      901 cgttccgctt tgcacagcga acggcacaag caaagcgcag cccgtcgttc gtcgggcgtg
      961 agataagcgt tccgcgagct catcgccagt ccgtccggtt ctcgtacgat cggatggccg
     1021 acgatttcca cttccatgct caggtcgcgc accattctgc gaatcacctg cagctgctga
     1081 tagtccttct taccgaagat cgccacatca gggcgaatca tgttaaacag cttcgccacc
     1141 gccgtcgcga cgccgcgaaa gtgccccggt ctcatcgcgc cgcagagcgg cagactgagc
     1201 ttctcgactt caacgtaggt ctcggcccca tccggataca tcgctccaag cgcaggatga
     1261 aaaacaatat cgatctgttc tttctccagc aggcggcagt cgcgctcgaa atcgcgcggg
     1321 taggcggcaa aatcttcccc tggtccaaac tgggttggat tgacgaagat cgacaccacc
     1381 actcgatccg cgtgcttttt cgcatcccgc accagagaaa gatgtccctc atgcaaaaaa
     1441 cccatggtcg gaaccacagc gatccgccgg ccggaacgcc attcgcgctc gctccaatca
     1501 cgcattgcgg aaaccgtctc gatgatctgc atggtggaaa taacctggag tattgggaag
     1561 acccgacacc ctaacactcc ttactccatc tcttcttact taaacgagtg ctcttccgtc
     1621 ggaaattttt tctctttcac atcgctgata tagcttttca ccgcgccgag cataatggat
     1681 ttaaggtcgg cataccgttt gacgaatttc ggctggaaat catcgaatag acccagcatg
     1741 tcgtgaatca caagcacctg gccatcgcag ccgatgccgg caccgatacc gatcgttgga
     1801 atcgtcaagc gccgcgtgat ttcctcggcc aagtccaccg gcatcccttc cagcaccagg
     1861 gagaaagcgc cggcgtcttc gaccgcgagc gcatcgtgca ggaccgcctc gcggcgctcc
     1921 ttttgccggc cttggacttt gtaaccaccg aattggtgca ccgattgcgg cgtgagaccg
     1981 atatggccca tgacgggaat gccgaccttc acgagcgctt cgatggtttc atgcatgata
     2041 acgccgcctt ccagtttcac cgcctcggcg ccgccttcct ggagaaaacg accggcgttc
     2101 tcgagcgcct tctctttact gacttgatag gaaagaaacg gcatatcgcc gatgacgagc
     2161 gcgcgtttgc gtccacgcgc aactaggcgg gtatggtaga tcatttcatc catagtcaca
     2221 gccagagtat tgtgatttcc ctggataaca caccccagcg agtcgcccac cagcagcatc
     2281 tccactcccg cgtcatccag gatgcgcgca aaagcgtaat cgtaggcggt caggcaagtg
     2341 atcttctcgc cccgttgctt cattttaacg atatgagggg cggttatctt ttcgcccata
     2401 gcgcgcctcc tgaaacaaaa aagccctccg gaactaatcc ggaaggccac agaggcaggg
     2461 aagagccgct ttctctcccc gagtatccgc ctttggcatc cgtctcagtc cagattacct
     2521 ggatccaagc ggtatgtagt gctgcgttcc cgaccccatg ctgttgattt ctttgatcaa
     2581 gtcagccaga tccttgctgc tcgacacgaa atcaatctcg gacgtgttca ccaccagcag
     2641 cggcgtttcg tcataatgaa aaaaaaaccg attgtacgcc tgcgccacct cgcccagata
     2701 ctcgaaagtc acgccacgtt cgtatttttt atctcttttt ttaatccttt tgtaaagcac
     2761 ctcggaacga gcttggagat aaaccaccag atcgggtttc ggcacgcgag cgttcaatag
     2821 agcatagatc tgttggtaga gatttagttc ttcggcgctt agggtcaggc tcgcaaaaat
     2881 ctggtctttc gcgaagagat agtccgccac cgtgttctga ctaaaaagat cctgctgcgc
     2941 cagttctact tgctgctggt aacgattgag tagaaaaaac gtctggtttt ggaaggcgta
     3001 ggtttcccgg gatttataaa atttcggcag aaacggattg tcctccaccc gctcgaaaac
     3061 cgtccgcgcc tgaaactcgt tggcgagaat ttttgccagg ctggttttcc ccacaccgat
     3121 gggtccctcg acgacaatat acttggcgtt acccatcggc acgagaattt acacgctgcc
     3181 cgaaagattg tccaggttgt ggtatattgt cgacgtgcag tggctgcgcc aaaagctcgg
     3241 gcagatgctg atggtcggct gccatggtga gaccctaact cccgatgagg agttagtttt
     3301 tgaagagtac gctttcggcg gttttattct cttccgacaa aactgccggt cgcccgagca
     3361 aatctcggat ctgtgtcgat ccctttggaa cacggcagcg gaggaaccgc cattcttagc
     3421 catcgatcag gagggcggtc gggttcatcg tctgccggag ccgtttaccc attttcccag
     3481 cgccgctatc atcggacaat ccggaaaccc ggaagtagcc tatcagtgtg gccgcgccac
     3541 cgccgcggag ttgacgattg ctggcctgaa cctcgatttc gcgcccgtgc tcgatgtcaa
     3601 ttcgaacccg gcaaatccca ttatcggaga tcgtgcgttt ggcaggacgc caaagcaagt
     3661 tatcgaaatg agttccgcct ggatgcgcgg tttgcgcgac ggcggcatca taccctgcgg
     3721 caaacatttt cccggccatg gtgacaccga gcgagattcc catctcgagc tgccggtagt
     3781 ggaaaaatca ctggatcagc tgcgaacgat cgagttgccg tctttcgtta acgcgtgccg
     3841 cgctggtata gaagcgctca tgaccgcaca cgtaagattt agcgcgctag accgtgagct
     3901 gcccgcgacc ttgtcggaag ccgtggtcac cggaatgctc cgccaccaat tcggttacga
     3961 cggcctcatt tttagcgatg acctggagat gaaagcgatc agcgccaact ttccggcggc
     4021 cgaagcggca gagcttgccg tgcgcgcggg agtcgacgtg ctgttgttct gtgatgaatt
     4081 aaacaaggcc atcgatgtgt tcgaatcgct ttgcgcgctg gcggagaacg atccggagct
     4141 gcgggcgaga atagaaacca gctttcgaag aattaccggc ttgaaacgac gcattctgac
     4201 ggcgtttacc gggttatcgg gcggggagag cttagctcgc ttggaagcga tgaaccatca
     4261 gcgcatcgtt gatcggattt acggcagctt gtaagccgta taggcgatca gccgcttgat
     4321 gtcgtggtgc agctcgccct cccatttttc cagcagcaag tcggcgggac acttgccctg
     4381 cgcgagcagg tccttgagcg gtttgaggta aatggtttcg tcttcaccaa gggcgtttac
     4441 ctgattttgc cggcgcaagc cttcccatgc gatctcgagt aattctctgg cgagatcgag
     4501 cagcgcataa cgccgcactc gggttgccag cgcatcgcga tgggaatcca agtatgcttg
     4561 catccgttca tcccagctcc agcctttcac cagatcccag gcggcctgca ggcaatcact
     4621 atcgtagaat gcgcccttga ccagagctga aagcgccggc atgagatccg gcggttgact
     4681 gtccaccgaa cggatctcga tatatcgttt gatgcgagtc tccgggaaca atgtggtcag
     4741 gtgatcgccc cagtcctcaa tggtggcgcg ctcgccatta tagccatcct caagaaattg
     4801 gcgaaacgtc atgtgggtca tgtcgatgta gttcttatta cgaatgataa agtacatcgg
     4861 cgcatcgagc gcgtactcca cataatgggc gaacgaagct tcgggggcga atgcaaactt
     4921 cagcaagccg gagcgattcc tgtccgtatc ggtccaaatg tgctcgcgaa agctccggta
     4981 gccgttgatc tcgccgtcgc agatcggcga attggcgaac atcccgatca aaaccggcgc
     5041 gagtcccatg ccggtgcgaa actttgcgat ggcatccttc tcgtcgttgt aatcgatatt
     5101 ggcttgcacg gtcgcggtct gcttcatcat gcgctggccg agcttgccga ccttgagcat
     5161 gtacggcgcc atgatccggt agcgtttttt cggcacccat tcgatctggt ccaaccggct
     5221 gaccggctgc atgccgagcc caagaaaaat gatgcccaag ggctcggcca cctctaataa
     5281 ttcgcggatg tgctggttga actcagcata ggtgcagtga atgctgtcgc acggctcgcc
     5341 gctgagctcg atttggccgc cgggctccaa tgtgatctgc gccttgtcgc gcgtgagcgc
     5401 gatgatgttg ccgtcctgtt cttccgcgtc ccagccgaag cgctcgatca gctcccgtaa
     5461 gatgaaagcg acaccgcgct tgccgaaata gggaatcgcc tggcccgtgt ctcgatagat
     5521 gccgactttc tcgtattcgg taccgacgcg ccacttgtcg cgcggcttgc cggcgtcatg
     5581 aaaataagct tcgagctcgt aatttttttc aacgacagac atgggttcga atgttctgca
     5641 ggtacggcgg atgaatcact cccttttcgg taatgattgc ggtgatcaga tcatgggacg
     5701 tcacatcgaa ggcgggattg aggatccgaa tccccttggg cgccagggct ttgccgaaaa
     5761 tatgcgagac ttctttgaca tcccgctcct cgattggaat atcctttccc gtcgcgcagt
     5821 tgatgtcgat cgaggcggtc ggtccagcga cataaaacgg aatcgcatgg cggcgcgcca
     5881 gcacggcgat cgtataggtg ccgattttgt tggccacatc accgttggcg gcaactcgat
     5941 cgcagccgac caccaccgca tcgatcttgc ctctctgcat cagataacca gccatactgt
     6001 cggtaatcaa cgtggccggg attttttctt tgcataactc ccaagccgtc agccgcgcgc
     6061 cttgtaaaaa aggccgcgtc tcgttcacga aaacctcgaa ttctttgcct gaatctttaa
     6121 gcgcgcgaag gacccctaat gccgtgccat agccggcggt cgccagcgcg ccggcgttgc
     6181 aatgggtcat gatttttttc gtattgcgca acagactcgc gccgaatttt cccagctgct
     6241 tgttcgccgc aatgtcttgg tgatagattt tctgcgcttc ttccttgagc gcgcgtttga
     6301 ccacgtctat gccttgactg cgcttgtccg aatagatctg ccgcatccgc tgtaaagccc
     6361 agaataaatt caccgccgtc ggacgggtct tcgccagagt cgagagaatc cgctcgaatt
     6421 cacgatcgaa agtttttggc ggtgctttga gtgcccctag cgccactccc atcgccgcgg
     6481 cgacgccgat cgccggcgca ccgcggatca ccatgtcgcg gatcgcactg gcgacttgct
     6541 gataatcgcg acagacgcgg tatacctccc gatgcgggag cagccgctgg tcgagcatca
     6601 ccactcgatc gttcttccac tcgatggttt tgacggtcat acgacgcttt cctaattgag
     6661 cgaacagcga gtatattaac agctcaaagg aagcgtcaag gggccgtgga tttctatcga
     6721 ctgcttggtt atttctccgg gctgcttttt gtcaaacccg aaatggatgt gagcgcgctt
     6781 tggcgtaccg ctgcattggt acattttctc gatgcgattc tctgctgtgt cattgccaaa
     6841 tacagcggcc gcagcaaaaa actgtggacc gtcgcgggcg cgatctgcgg catctgggct
     6901 ttggcggtga tctttttgct gcccgaaaag aacgatctga aagctccgcc caggagcgaa
     6961 gagcagacaa agacgttcaa atagttttag ccggccaagc gttcaataaa tgcgtgtacg
     7021 ggcgcagcat gcggcgccct gcgctttggt ggcccatcct actcttcggc tatttttttc
     7081 tcttcggcta tttttctccc gagtcgtcct cggccacgac gaacgcgaga ccgtgatctt
     7141 tggtgtgcgt gattgaaagc gcccagcggt gaattcctag ttcttgggcg agccgcgcgg
     7201 tcttatcgtg gagaacgatc gccggtttgc cgctgcgtgc gcgcgccact tcgatatcga
     7261 gccaacgaac tttggccccc cagccgcggc cgagcgcctt catcaccgct tctttagctg
     7321 cgaaccgccc ggcgtagctt tcatatttgc cgcgctgctt tttctcgcag tattcgatct
     7381 cctcaggggt gaagacacgc tcgcgaaagc gcgcgccgat gcgcggatct tcgagggcgg
     7441 cttgaatccg attcacctcg gccaaatcga cgccggtgct gacgatcatg cctgcgcctc
     7501 gcgtgcgctg ggtgatgcac cgtgcgctag tccccagcgg cacatgctgt ctaccactgg
     7561 tttcaagctt gtgccgagtg gcgtgagcga atactccact cggggtggaa cttcggcgaa
     7621 aattttcctt tcaaccaaac cgtcacgttc catttctctc agctgctgag tgagcatctt
     7681 ctgtgtcacc ccgcgcaaag cccgggcgag ctcggaaaac cgtttggcgc cattcaacag
     7741 atgaaatatc agcgggatct tccatcggcc atgaatcaga cgcagcgtat actccgccgg
     7801 gcagctagtt tcttttggtt tcatacagag ctccgcggtt acttgaaagt tactatagca
     7861 ccagccggtg cgtacttaac atcaagcgag cggatgatta cattccgctg gctaggagca
     7921 actatgcgaa ccgccgtatt gaaaaaagtc cttgatcatt atccgaccgg caaagctacg
     7981 cccaatccgc cgctcgcatc tgaccgcgat gcagccttgc tcgacgcgta ctcccaggcc
     8041 gtcgtctatg ccgcggaaaa ggtcagtcct tcggtcgcct tcattgaagt cagcaaagcc
     8101 ctgcctcaac gtcgatccac ggtcgaacga aggcccgaag ctcgcggtag cggttccggg
     8161 ttcgttttta cgccggacgg ttttattctc accaacagcc atgtggtaca aggcgcaagc
     8221 aaaatcgccg tcaccctgag cgatggacga cgcttcgacg cgagcctcgt cggtcacgat
     8281 tcgggcaccg atgttgccgt cattcgaatt tctgcgccgg ctctggtgcc cgcgcccctc
     8341 ggcgattcgg aaaaaatcag ggttggacag ctcgcgatcg cgatcggcaa tcctttcggg
     8401 tttcagtata gcgtcaccgc cggagtggta agcgcattgg gtcgctcgct ccggtccgag
     8461 tccgggcggc taatcgatag cgtcattcag accgatgccg cgctcaaccc gggaaattcc
     8521 ggcggaccac tggtcaattc caatggggaa gtgatcggca tcaacaccgc tgtcattctt
     8581 cctgcacagg gactctgctt tgcggtggcg gtcaataccg caaaatttat cgccggccac
     8641 ctcatcatgc acggacgcat tcgccgcgcc tatcttggcg tcggcggaca aaacgtcgcg
     8701 atcccgcgct tcgtggtgcg cgcgcaacag ctccagaccg aaagcggcgt gctggtgatt
     8761 tccagcgaca aagaaagtcc cgccgcgcag gctggactta aagatggcga cgtgattgtc
     8821 ggactgggtg attcgccggt gcgctcggtg gacgatctgc acaagctttt gaccgaagca
     8881 cggatcggct cgcgttgcga gctcaccttg ctgcgccaaa gccagaaaat ctcgctcacg
     8941 gtcatagccc aagaggcacc cgcctgaccg cgccagcggc cgtggtgctc acgtcagggc
     9001 ctcggtctcc ggcaaatctt tagtggcgcg cccgattgcg atttgttatc gtggatcatt
     9061 ctaaaatcaa gggagtgacc cacgtgctga aacgtttctc attgacgttg ttattgataa
     9121 tcatttttgg cctggcggga acggcagcgg cccagcagaa gggcagaatc aaaattgccg
     9181 tgcaagcgcc gttatccggc gaacaggctg ccatcggcga gcacatcaag cttggcgctc
     9241 agctcgctgt ggaagaatcg aacaaagcat tcaaagatct cggctacgat ttgatactcg
     9301 tgccgcagga tgaccaggct aaaccggagg ttggcgtcgc caatgcgcga aatatggtcg
     9361 ccgatccgga ggtgctcgtg atcgtcggac attttaattc cggtgtcgcg ctgccctctt
     9421 ctgaagttta caaagacgcc atgctgacga tgatttcccc ggcgaatacc gcgaccgaaa
     9481 tcaccgatcg cggctatcca aatgtgaatc gggtgtgcgg gcgcgacgac gttcaggggc
     9541 ccgtcggcgc tcggttcacg gcccaagatt tgaagttgaa gtctgtttac atcatccacg
     9601 acaagaccct ctacggccaa ggtgttgcgg aaaattttcg caacgaagcg aagaagctcg
     9661 gcctcaccgt cttgggtttc gacggcactg aagagcgcgc caatttttcg ccgatgatca
     9721 ttccgctcaa agccaaaaat cccgatctgg tctacttcgg cggcatttat caccagggcg
     9781 gacttttgct gaagcagctc cgcgagaaag gcgtcaaggc gaagttcatg ggaccggacg
     9841 gactcgattc ggccgagatg gccaagatcg ccgggagtgc tgtggttgga agctactaca
     9901 ccagtgtcgc gggtccgccg gatgcctatc ccgaaaccgc cgccttcgca aaaaaatata
     9961 agcagcggtt cggaaaagag acggagtcct tcggcatgta cggctatgac gcgaccttgg
    10021 tcgggatcaa agccatagag ggatggatca aagccaaccc tggcaaaaaa ccgagccgtg
    10081 ccgatgtcgt cacggcggtg cgcaacatca agaatttcaa aggtgtcaca ggctccatcg
    10141 aattcgacaa caaaggagat ccggtaaaag ccaagtactt cgtcctacag ttcgaaaaac
    10201 aagcttaccc cggcaaagtc gtgaaggtga tcgaacagca agcgccggcg gcagtcgcga
    10261 ggaagtcgta ggctgactga caagccgagg atagaagata gaggattgaa gatcgaatcc
    10321 atcctccatc ctcagtctct atcctcgatg ttccatcctc atcctcggcc cagtcctgaa
    10381 aattggcaat gaggaaaaaa ttgcgcgtcg cactgctgtt cggtggcaaa tccgccgagc
    10441 atgagatttc gctggtgtcg gcgcgaaata tcgccaaggc catggacaag ggaaaatatg
    10501 acctggtcgc cattggcatc gaccgccaag ggcgctggca cctcgatgaa ggcgcgcgcc
    10561 tgctcttcga cgaaagacgc actacggtcg aattcacaga gcgaaagact ctggccgcgg
    10621 tacggcctgg agagaactca acgcctttgc tgcgcgaagg cggcgctggc attggcgtca
    10681 tcgatgttgt ttttccagtg ctgcacgggc cgttcggcga agacggcacg attcaaggat
    10741 tactcaagct cgcggatctg ccgtttgtcg gcgctggcgt tttgggatcc gccgtcggta
    10801 tggacaagga cgtgatgaag cggctactgc gtgacgccaa aatcccgacg cctaagttcg
    10861 tggttttcga aaaacgggaa aaaaaatcca tccagttcaa aaccataaaa aacattctcg
    10921 gtctgccatt ttttgtgaaa cctgcaaacc tgggatcttc gataggaatc agcaaagtga
//
LOCUS       DASULZ010000647         2819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_376684, whole genome shotgun sequence.
ACCESSION   DASULZ010000647 DASULZ010000000
VERSION     DASULZ010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376684"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VFY96_13425"
     CDS             complement(<1..658)
                     /locus_tag="VFY96_13425"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607518.1"
                     /translation="MNRAAVMASAALFLSCGLNLAQAQAPKKVKITIPVIAHSMTPVF
                     IAQNKGFFTEEKIDIDVTSTNGGGPDIRALIAGDVDFSFTTGDNVILAQQEGKKLVMV
                     MSGLNKLFINWAMHKDVAKSKGVTESMPLAEKIKALKGLTIGVTNPGALTAHLAAFVV
                     RKAGYNPQQDVNIIPIGSGPTWLAALENHKVDVALTAPPVPDTAISRGFAILLINNAK
                     G"
     gene            complement(676..1449)
                     /locus_tag="VFY96_13430"
     CDS             complement(676..1449)
                     /locus_tag="VFY96_13430"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5607519.1"
                     /translation="MALLSIEGVGKDFFARGKRVLAVEKVDLTIEEGEFVTVVGPSGC
                     GKSTLLNLCVGLLRASSGQILFRGIPIDGICTKIGYVTQKDNLLPWRTLVENVEIGLE
                     IRGVEEKTRRRSAEEWVERVGLKGFEEHYPHELSGGMRQRANIIRTLIYDPELILMDE
                     PFGPLDAQTRIVLQDQLLKLWLTTRKTIIFITHDLVEAITLADRVVLMSSRPGKIKSV
                     ETIDIPRPRDVFKIHEDPRFRSAYEKLWQQLRPEVTLDA"
     gene            complement(1473..2453)
                     /locus_tag="VFY96_13435"
     CDS             complement(1473..2453)
                     /locus_tag="VFY96_13435"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5607520.1"
                     /translation="MNIDLHSHFFPVEALQSPGRYESHAPKLVLENDKLSVTSQIGMR
                     AGLGAGAYDPAARIKALDQMSIDLQAISPSPILLFYWDEPDVAAHFSRRQNEAIQMVV
                     QKFPARFVGFGSVPLQSVPQAIAIAEEAKSMGLKGLEIGNAVGEKPIDDPSFEPFFTA
                     AQKLDLLLFVHPLEGGLDSGDPLAPILGNVLQFTYRTTMMIERMILNGMFERFPNLRL
                     CLSHGGGLLAFNIWRLDHSYGLRPELKRSVPRKPSEYLKRFYFDTIVHSVAALQYLIN
                     VVGADRVVMGTDYPMAMGDFEPVKKVAETNLTASERELVLGQNGLRALAL"
BASE COUNT          659 a          808 c          696 g          656 t
ORIGIN      
        1 cccccttggc attattaatc agcaggatcg cgaagccgcg gctgattgca gtatccggca
       61 ctgggggcgc cgttaaagcg acatcgactt tgtgattttc cagcgctgcc agccatgtcg
      121 gcccggagcc gatcgggatg atatttacgt cttgctgagg gttgtaccca gctttgcgca
      181 cgacaaaagc cgcaaggtgc gcagtcaacg cgccggggtt ggtcacgcca atggtcagtc
      241 ctttcagcgc ttttattttt tccgccagcg gcatcgactc tgtaacccct ttggattttg
      301 ccacgtcctt atgcatcgcc cagttgatga aaagcttatt taggccgctc atgaccatga
      361 cgagcttttt cccttcctgt tgcgcgagga tcacattgtc accggtagtg aaactaaaat
      421 cgacgtctcc ggcgatcagc gcgcggatat ccggcccgcc gccattggtg gatgtcacgt
      481 cgatgtcaat cttctcttcg gtgaaaaaac ctttgttctg cgcaatgaac accggcgtca
      541 tggagtgcgc aatcacggga atggtaatct taactttctt cggcgcctgc gcttgcgcca
      601 agtttagtcc gcaactcaga aagagcgcgg cgcttgccat tacggctgct cggttcatgg
      661 tcattctcct tttcgctagg cgtccaaagt aacttcaggg cggagctgtt gccaaagttt
      721 ctcgtacgcc gaacgaaatc gcgggtcctc gtgaatttta aagacatccc tgggtcgcgg
      781 tatgtcgatt gtctcgacgg atttgatttt ccccgggcgc gagctcataa gcacgacacg
      841 gtcagcgagg gtgatcgcct cgacgagatc gtgcgtgata aaaatgatcg tcttgcgtgt
      901 cgtcagccag agtttcaaga gctgatcctg tagaacaatg cgcgtctgtg catcaagcgg
      961 tccaaagggt tcgtccatca aaatcaactc gggatcataa atcaatgtcc gaataatgtt
     1021 ggcgcgctgg cgcatgccac cggagagctc gtgcgggtaa tgctcctcga aacctttcaa
     1081 gccgactcgc tcgacccact cctctgccga gcgccggcgc gttttctctt ccactccccg
     1141 aatctcgagt ccaatctcga cgttttctac cagagtgcgc cacggcagca aattatcttt
     1201 ttgcgtgacg tagccgatct tggtgcaaat gccgtcaatc ggtattccgc gaaacaaaat
     1261 ttgtcctgag cttgcacgga gcaatccgac gcacaaattt aacaatgtcg acttgccaca
     1321 ccccgacggg ccaacgaccg taacgaactc gccttcttca atcgtcaaat cgactttttc
     1381 aaccgcaagc acccgtttgc cgcgcgcgaa aaagtcttta cctacaccct cgattgaaag
     1441 taacgccact ccgaatctcc ggcgctgcca agttacaaag ccaatgcccg caagccgttc
     1501 tggcctagaa ccagctcccg ctcactggcg gtaagattcg tttctgcaac ctttttgacc
     1561 ggttcgaaat cacccatcgc catcggataa tcagtgccca taaccacccg atcggctccg
     1621 acgacattga ttaagtattg aagtgcggca actgaatgga cgatcgtatc gaaataaaac
     1681 cgcttcaaat actcagaagg ctttcgcggc accgatcgtt tcagctcggg tcgcaatccg
     1741 tacgaatgat cgagacgcca gatgttgaac gccagcaaac cgccgccatg ggatagacaa
     1801 agccgaagat tcggaaaccg ctcaaacatg ccgtttagaa tcatgcgctc aatcatcatc
     1861 gttgtacggt aggtgaactg gagcacgtta cccagtatcg gcgccaatgg atcaccggag
     1921 tcgagccctc cttccagagg atgcacgaac aagagcaggt ctagtttctg cgcagcagta
     1981 aaaaagggtt cgaagctcgg atcatcgatg ggcttttcgc ccacggcatt gccgatctcc
     2041 aatcccttca atcccatgct cttcgcctct tcggcaatcg ctatagcttg cggaacgctt
     2101 tgcaagggca cgctgccaaa gcccacgaag cgagcgggga atttctgcac gaccatttgg
     2161 atcgcctcgt tttgccggcg cgaaaagtgc gccgccacat ccggctcgtc ccaatagaat
     2221 agcaatatgg gcgatggaga aatcgcctgt agatcgatgc tcatttgatc cagcgctttg
     2281 atccgtgccg ccggatcgta agcgcccgca cccagtcctg ctcgcatacc gatctgtgag
     2341 gtcactgata atttatcgtt ctccagtacc aacttcgggg cgtggctctc atacctaccc
     2401 ggactttgca gcgcttcaac cggaaagaaa tgggaatgca gatcaatatt catgagagcc
     2461 gcgccgctat ccgaatagtt caaacggaac cacctataca ccaactgctt ccgttgcact
     2521 agtcacagcc ggcacaacgt acagtttgat atggctatga acagacaaac cgccatcgct
     2581 ctggtatggc tgacttgacg aagccagccg gtttgccata ttcaacgtca taagatcaac
     2641 ggctgctata ctgaccaatc acaaggaggc gttatgggat tgctcaaagt cgaaggggtg
     2701 acgcactggt ccatcccggt aaataattta gacgaatcgg aaaaatttta tggcgatctg
     2761 ctcggactta aacacctcgg ccgcctcggc aactccggga tgtcctgctt caacgtcgg
//
LOCUS       DASULZ010000648         3452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1190063, whole genome shotgun sequence.
ACCESSION   DASULZ010000648 DASULZ010000000
VERSION     DASULZ010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190063"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VFY96_13440"
     CDS             complement(<1..560)
                     /locus_tag="VFY96_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5607521.1"
                     /translation="MNSPAKILVVDDTPKNVKLLADLLSVKGYAVVTASSGKEALTRL
                     NTERPDLVLLDVVMPEMSGYEVCAAIRADEETAVLPVVMVTALDPGVERVKGIEAGAD
                     DFLSKPINQPELLARVRSLLRIKALQDEVQSQAAQLAELNNSLERRVQEQVAELNRLG
                     RLRRFFSPQIAELIVNGAADDPLKTHR"
     gene            complement(632..1015)
                     /locus_tag="VFY96_13445"
     CDS             complement(632..1015)
                     /locus_tag="VFY96_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5607522.1"
                     /translation="MANELILIIEDNEKNRKLCRDVLNVKGYSTIESETAEEGLKLAR
                     EKSPSLVLMDIQLPGMDGITAMKQLRAAPETAQIPVVAITASAMTHNRATMLAEGFDG
                     YQTKPISLKDFLAEVQRVLAGRKKP"
     gene            complement(1025..>3452)
                     /locus_tag="VFY96_13450"
     CDS             complement(1025..>3452)
                     /locus_tag="VFY96_13450"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX5607523.1"
                     /translation="MEMRPFTPAQIKLLETFADQAVIAIENVRLFQELKESLEQQTAT
                     SEILGVIASSPTDIQPVLNTVAKNSARLCEAADAQIRLVEADRTRLVASFGSLPAPEF
                     AAITPLNPSGRAILTRQTLHIHDLQERKDEFPESQSLRRGLRTFLSEPMLREDTAIGV
                     INVRRTEVRPFSDKHVKLLKTFASQAVIAIENVRLFKELEERNRQITEALEQQTATSE
                     ILRVIASSPTDIQPVLDTVAENAARLCEATDAVITRADGESVKQVAQFGAIPVPEPNR
                     INRHSPVSRAIVDKKTIHVHDLLAEIETEYPESKERQKVTGTRTILVTPLLREGLPIG
                     TINIRRTEVRPFSEKHVKLLETFTDQAVIAIENVRLFQELEARTQQLARSVEELKALG
                     DVGQAVSSTLDVQTVLSTIVARAVQLSGTDCGIIYEFDEATKEFHLRASYQMEEELVK
                     AYRATPLRFGEGATGRAAETRMPTQIAELREEQEFATRGMRPILMRLGYRSLLAVPLL
                     LEQRIMGALTIYRRRSGDFAPEIINLLRSFATQSVLAIQNARLFREIETKSRALEAAN
                     RHKSEFLANVSHELRTPLNAIIGFSEVLLERLFGELNDKQNEYVDDILSSGRHLLSLI
                     NDILDLSKIEAGRMELERATFDLPLAIENALLLVRERASRHNIRLICAIDERLGDFTG
                     DERKVKQILVNLLSNAVKFTPEGGKIRVDAGMGDSAVIISVTDSGIGIAKEDQEVIFE
                     EFPQASGNYAQKREGTGLGLTLTRRFVEMHGGKIWVESEIGKGSKFTFTLPAKPNIAE
                     TVSGLVVDER"
BASE COUNT          720 a          971 c          898 g          863 t
ORIGIN      
        1 cgatgagttt tcagtggatc gtctgccgcg ccgttgacga tcaattcggc aatttgcggc
       61 gagaaaaaac gcctgaggcg gcctaaccgg ttgagctcag cgacttgctc ttgaacgcgc
      121 cgctccaagc tattgttcag ctccgccagc tgcgcagctt gactctgaac ctcgtcttgg
      181 agcgccttga ttcggagcag cgagcgcacg cgggctagaa gctcgggttg gttgattggc
      241 ttcgacaaaa aatcgtcagc cccggcttct atgcctttca cccgctccac acctggatcg
      301 agcgccgtaa ccatcaccac cggaagcacc gcggtctcct cgtcggcgcg gatcgcggcg
      361 caaacttcgt aaccgctcat ctcaggcatc actacatcca acagtacaag atccggccgt
      421 tccgtattta atcgcgtcag tgcttccttt ccggagctgg ccgtgaccac agcatagccc
      481 tttacgctta acagatcggc aagaagcttt acgttctttg gcgtgtcgtc aacaacgaga
      541 attttcgcgg gactattcac gcttataact cagttagggt tagggtaagg ggtaagcgaa
      601 tggagaacgc ctcatcactt agcctgacga cttacggttt ttttcgccca gcgagcacgc
      661 gctgcacttc ggccaaaaaa tccttcaagc tgatcggttt agtctgataa ccgtcaaatc
      721 cttcggccaa catggttgcc cgattgtgcg tcatcgcgga ggcggtgatg gcgactacag
      781 gaatttgcgc tgtttcaggg gcagctcgaa gctgcttcat tgccgtgatt ccatccatcc
      841 cgggaagttg gatatccatt agaaccagac tcggactttt ctcacgcgcc aatttgagtc
      901 cttcctcggc agtctcggac tcaatcgtgc tgtaaccttt gacattcagc acatcacggc
      961 agagcttccg gttcttctca ttgtcctcga tgatcaatat cagctcattg gccatagggt
     1021 tcccttatcg ttcatctacg acaagaccac ttaccgtttc ggcgatattt ggtttcgcgg
     1081 gtaatgtgaa cgtaaattta ctgcccttac ctatttcgct ctcaacccag attttgccac
     1141 catgcatctc cacgaacctt cgtgtcagcg tcaaaccgag tcctgtgcct tcgcgcttct
     1201 gcgcataatt tccgctggct tgcgggaact cttcgaagat cacttcctga tcctcctttg
     1261 cgatgccgat gccgctatcg gttaccgaaa tgatcactgc gctgtctccc atgcctgcgt
     1321 ctactctgat cttgccgcct tcgggcgtga acttcacggc gttggaaagc aaattgacta
     1381 aaatctgttt caccttgcgc tcgtctccgg tgaagtcacc gagccgctcg tcgatagcgc
     1441 aaatgagtct gatattgtgg cggctggcgc gttcacggac gagcaagagc gcgttctcga
     1501 tcgccagggg caaatcaaaa gtcgcccttt ccaactccat tcgtcccgct tcgatcttcg
     1561 aaagatcgag aatatcgttg atcagcgaca gcaaatggcg tccggaggag agaatgtcgt
     1621 caacgtactc gttctgcttg tcgttcaact cgccaaagag tctttcaagc aaaacctccg
     1681 agaagccgat aatggcattg agcggcgtcc gcagctcatg tgaaacattg gcgaggaatt
     1741 cggatttatg ccgattcgcc gcctccagcg ctcggctctt ggtttcgatc tcacggaaaa
     1801 gcctggcatt ttgaatcgcg agaaccgatt gcgtagcaaa gcttctcaag agatttataa
     1861 tttccggcgc aaaatcgccc gaccgccggc gataaatggt caatgccccc attattcttt
     1921 gctccaggag tagcggcacc gcgagcaaag atcgatagcc gagccgcatc aaaatcggtc
     1981 gcatacctcg ggttgcgaac tcctgttctt cccgcaactc ggcaatctgc gtcggcattc
     2041 ttgtttccgc cgctcggccc gtcgcccctt cgccaaaacg aagcggtgtc gctcgataag
     2101 ctttgaccag ttcctcttcc atttggtagc tggcgcggag atgaaattcc ttcgtagcct
     2161 cgtcaaattc gtaaataatc ccgcagtcgg tgccggacaa ttggaccgcg cgagcaacga
     2221 ttgtactgag taccgtctgg acgtcgagag ttgagctgac cgcctggccg acatctccta
     2281 aggctttcag ctcctcgacg gaacgcgcaa gctgttgcgt ccgcgcctct aattcttgga
     2341 acagccggac attctcgatt gcgatcacgg cctggtcggt gaaggtttcc aaaagcttaa
     2401 cgtgcttttc tgagaagggg cgcacttccg tacggcgaat attgatggtg ccgatgggca
     2461 aaccttcgcg cagcaacggt gtgacgagaa tggtgcgagt gccagtaact ttctgacgct
     2521 ctttcgattc cggatactcg gtttcaattt ctgccagcag gtcatgaacg tgaattgtct
     2581 ttttgtccac tatagcgcgg ctgacgggcg agtggcgatt gatgcggtta ggttcgggga
     2641 ccgggatcgc tccaaattgt gcaacttgtt taacgctttc cccatctgcg cgggtgatga
     2701 cggcatccgt cgcttcgcac agtcttgcag cattttccgc aaccgtatcc agcaccggct
     2761 gaatatccgt tggcgagctg gcgatcacgc gaaggatctc gctggtcgcc gtctgctgct
     2821 cgagggcttc cgtgatttgc cggttgcgtt cttcgagttc cttgaacagc cggacgtttt
     2881 cgatggcaat gaccgcctgg ctagcaaagg ttttgagcaa tttgacatgt ttgtccgaga
     2941 aaggacgaac ctccgttcgc ctgacgttga taactccaat tgcagtgtct tctctgagca
     3001 tcggctcgct gagaaacgtt cggaggcccc ggcgcaagct ctggctttct ggaaattcgt
     3061 ccttgcgctc ctgaagatcg tgaatatgca aagtctgtcg cgtaagaatc gctcggccac
     3121 tgggatttag tggagtgatg gcggcaaatt ccggcgctgg tagagagccg aacgaggcca
     3181 ccaatcgcgt tctgtcagcc tcgacgagtc gaatctgcgc atccgccgcc tcgcagagtc
     3241 gggcagaatt tttagcgacg gtgttcagaa ccggctggat atccgtgggc gagctggcaa
     3301 taacgcccaa aatctcacta gtcgcagttt gctgctccaa tgattccttg agttcttgaa
     3361 acaaccgtac gttctcgatc gcaataacag cttgatcggc gaaagtttcc agtagcttga
     3421 tctgcgccgg ggtgaagggg cgcatctcca ta
//
LOCUS       DASULZ010000649         8980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_682412, whole genome shotgun sequence.
ACCESSION   DASULZ010000649 DASULZ010000000
VERSION     DASULZ010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8980
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682412"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VFY96_13455"
     CDS             <1..1081
                     /locus_tag="VFY96_13455"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5607524.1"
                     /translation="EGAIEGPIVGHLLDRYGPRPVMLAAVLLMGAGYLLLSQVDSYIA
                     FLIVYTGLISLTHSGGFMHAPMALTNTWFIRLRARAMTINSAAYGLGGVLLAPILSIV
                     VHAWGWRAGAALAGMTFLLVGIPLCLTIRSSPESMGLSPDGDPYAGEITDEKHSAQPR
                     SVELNVTAAQAIRSFAFWALVFGAGVRNASYHAISTHFIPMMIWKGMSQQQATFLLSS
                     FALLGFISTLVFGWLADNVNKPRLVSSILFTAGVSILVLIFGSSIGLLWIFILLFTTV
                     EATYPVAWALVGDLFGRKHFAKIRGYMSVFYVWGSVVGPVIAGAVWDRWQTYQPMLWI
                     LVAMFFISGIFYSLLGKPWAQPKPA"
     gene            complement(1110..1430)
                     /locus_tag="VFY96_13460"
     CDS             complement(1110..1430)
                     /locus_tag="VFY96_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011790595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5607525.1"
                     /translation="MFVEVKNIFADLPQQIGAENFFRLFQSRSTNIERIVSHSHCSPP
                     DFWYDQPEDEWVIVLRGHATLDFEAGGSVELKAGDYVTSPRHVRHRVARTSEETLWLA
                     VHSK"
     gene            complement(1433..2104)
                     /locus_tag="VFY96_13465"
     CDS             complement(1433..2104)
                     /locus_tag="VFY96_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX5607526.1"
                     /translation="MATPLDILVTGGTGYIGRNLIPPLIARGHRVRVLAREDSIARVS
                     PGAAAIIGDALDDRSVATALHTGDTLIHLVGTPHPTPSKADQFEKIDLISIRASVNAA
                     RHVGIDHLVYVSVAHPAPVMQAYLWVRTLGETMIREAGITASILRPWYVLGPGRQWPK
                     LIVPLYKLAELFPGTRSTAERLGLVTIEQFMTAMISAAENPPIRGQRRIIDVPAIKRA
                     RLVKG"
     gene            complement(2143..3159)
                     /locus_tag="VFY96_13470"
     CDS             complement(2143..3159)
                     /locus_tag="VFY96_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HEX5607527.1"
                     /translation="MKRIVICFDGTWNRPADEKLPPGRQVETNVCRFYTSVKESGADG
                     IKQLKWYDEGVGTQWYDRFIGGGIGAGLELNIVQGYEFLGKAYQEGDEVYVLGISRGA
                     YTARSLVGMIRNCGLILPKHLTLRVPMAYGIYRTRGDSVDSFTAKVFRAAFSCEIKIK
                     FLGVWDTVGALGVPLHVLQDVNMKFYEFHDTNLSGIVENAFHAIAVDEHRIDYQVCLW
                     NPDAPAQQKLEQRWFIGAHCDVGGGYPDRRLSDLTLRWIQEKASMVGLALNPVGIGTE
                     NYRGVLTDSYAQFLSGLYAQKKPRHYRGIGTTQFGNEIVDQSVQRRRNDDPSYKPQNP
                     GLPN"
     gene            complement(3317..5026)
                     /locus_tag="VFY96_13475"
     CDS             complement(3317..5026)
                     /locus_tag="VFY96_13475"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX5607528.1"
                     /translation="MARSNSDGCDVCIVGVGGLGGIMAKQLASAGLKVVGFERGPAPK
                     LEDYAPRDSIRFLIRPEQLEWVRHEPTTTRNKTGDKSRLQYRTSPLNVLGGALLHWTG
                     QSSRYMPGDFRLFTNEIESGNAERAGANLTGYDIIDWPLSYDDLEPYYERFEWEFGIS
                     GEAGANPFAGPRKRGLPLPPLRHSARMNLFAEACKKLGYHPYDTAAGILSRPYRPSEP
                     FDSRIPERPACVYCGHCNFYGCHVHAKAATLYTTIPVALETGNFDLRTRSKVFCINTD
                     GDERVKGVSYFDAAGRARELRARVVILSAFVFEHVRLLLLSKTGRGRFARGLANSSGY
                     VGRNIMAHGDVRAMGAFDDYTINGFIGPGSAAMRIDDFNGNNFDHTGLGFIRGGTIGT
                     SGDGTPITRFDVLPPDVRRWGREFKDFYMRYYTRIMDLNMQPETLPHKGNRVDLDPHH
                     RDRWGVPLPRVTFDFHQNERRLQKFMAGVGEKIMRATGASKVWTEEKGRPNRWAGGTR
                     MGADPNNSVVNEFCQTHDVPNLFIVGSSVFPTMAGYPPTATVAALTYRTAEYILSQKQ
                     WFD"
     gene            complement(5029..5541)
                     /locus_tag="VFY96_13480"
     CDS             complement(5029..5541)
                     /locus_tag="VFY96_13480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEX5607529.1"
                     /translation="MDSMNHCDNVLTADESKILAAIADRIFPRTETAGAVEIGAVQYI
                     EVALAGDYAAFAPFYRQGLRAIERCAKAKFRRAFCLLSEAQKDQVLVDFESGAVPGFK
                     PAADFFETVRCHVLEGVFCEPQYGGNRDMIGWRLVNFPGQQFGYADAYINKPVDLEPV
                     AVDSPKEQGN"
     gene            complement(5634..6233)
                     /locus_tag="VFY96_13485"
     CDS             complement(5634..6233)
                     /locus_tag="VFY96_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX5607530.1"
                     /translation="MMTKRPERSICVFTGSRHGGSPEYAQAAAECAGELVKRGFGVVY
                     GGGNVGLMKVLANTALEAGGYVIGVIPDALVTKEVAHTGLSELRVVQSMHERKALMAE
                     LAAGFVALPGGIGTMEEFFEVLSWAQLGLHNKPCGLLNVDGYYDSLINFLDHAVAQDF
                     VKPKHRALLMIENQVMQLLDRFKTILAAPPGKKFDASIT"
     gene            complement(6269..7876)
                     /gene="clcA"
                     /locus_tag="VFY96_13490"
     CDS             complement(6269..7876)
                     /gene="clcA"
                     /locus_tag="VFY96_13490"
                     /inference="COORDINATES: protein motif:HMM:NF003640.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HEX5607531.1"
                     /translation="METEPQGEAKPASAFIEAKSEVQDYLAVSQQRHKLFPRAAAVGL
                     AAGGVAVVFRALLAAGDALRNGLVHWSYSTPAFGWIFPLVFGAAGAAAAVCLVVSYAP
                     ETSGSGIPHLKAVLHRLRDLSWMRVLVVKMISGVLAIGSGLALGREGPTVQMGGAVAD
                     GLARSMKVSSADRLTLTAAGAGAGLAAAFNAPLSGLVFVLEEMRRDFRPAVFGAAFLA
                     AASADVVARSVSGQLPVFTVPNYAMPSLLALPAFAVLGIAAGGFGVAFNRSLLEGVDF
                     AARFNGRQRVALAASVGVAAGLVAWFYPVAAGGGHELGEMVLTNRIGIIAVPILFLLR
                     FALTVASYGTGSAGGIFSPLLVLGALLGLGVGHLAHGLMPVLAPEPGVFAVVGMAAYF
                     TAIVRAPLTGILLITEMTGSYEQMLPLLTSSFCAYAVAEYLRDLPIYEALLERDLERG
                     GFGNERAQPIVAEFEIELDAPFAGRLVRELGLPSGCILVRCALDHTEQVVTANTRLQS
                     HMKITAVIAPEADNALEILRRGCTSTA"
     gene            complement(7912..8598)
                     /locus_tag="VFY96_13495"
     CDS             complement(7912..8598)
                     /locus_tag="VFY96_13495"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX5607532.1"
                     /translation="METILFDIDGTLTDTMDVDTRCFLQAFVDVCGFADVDPDWSRYR
                     NTTDAGIFQEVFESRRGRSPTASETVQFRDYLVALFRSAALQGPFTPIRGALDLLTRL
                     RERGEYRIGLATGSWSDAARIKMAGAGFCYDDYPEASADDAPDRETIVRIAAARTAGE
                     TWKEWNRTIYVGDGVWDVKMSRLLGIPFLGIGAGAQREKLLAAGASYVLPDFADLDQF
                     LFVLDKLESQ"
BASE COUNT         1956 a         2693 c         2445 g         1886 t
ORIGIN      
        1 ggagggagcc atcgaagggc cgattgtcgg tcacttgctc gaccgttatg gccctcgccc
       61 agtgatgctc gcggctgttc tgttgatggg cgccggttat ttgttgcttt cccaggtcga
      121 cagctacatc gcctttctga tcgtctatac agggctgatt tcgctcacac actccggcgg
      181 tttcatgcat gcaccgatgg cgttgaccaa cacctggttc attcgtctgc gcgcccgcgc
      241 catgaccatt aacagcgcgg cctacggcct aggcggggtt ttgctcgcgc cgatcctgag
      301 catcgtcgtg cacgcctggg gctggcgcgc gggcgctgct cttgcgggta tgacgttttt
      361 gctggttggc attcctctat gcctgacgat tcggtcttcg ccggaaagca tgggcttgag
      421 ccccgatggc gacccatacg ccggtgaaat aaccgatgag aagcactctg cgcaacccag
      481 aagcgtggag ttgaacgtca ccgctgccca ggcgatccgc tcgttcgctt tttgggcgct
      541 ggtgttcggc gccggcgtcc gaaacgcgtc gtatcacgcg atctccaccc atttcattcc
      601 gatgatgatt tggaaaggca tgagccagca gcaagcgacc ttcctgctga gctccttcgc
      661 gttactcggg tttatttcta ctctcgtatt cggctggctt gcggataacg tcaacaagcc
      721 gcgccttgtt tcatcgattc ttttcacggc cggcgtttct atcctggtgc tgatcttcgg
      781 cagctccatc ggccttctgt ggattttcat cctgctattc accaccgtcg aggcgactta
      841 tcccgtcgcc tgggctttgg tcggggatct gttcggccgc aagcacttcg ccaaaatccg
      901 cggctatatg agtgtcttct atgtgtgggg cagtgtggtc ggaccggtaa ttgccggcgc
      961 ggtctgggac cgttggcaaa cttatcagcc aatgctctgg attctggtcg cgatgttttt
     1021 tatttcaggc attttttaca gcctgctcgg gaaaccttgg gcgcagccga agccggcgtg
     1081 agaaatcctt cacttccgtc agaacaagct tatttgctgt gcaccgctaa ccaaagggtt
     1141 tcctcgcttg tgcgcgcgac ccggtgccgc acgtggcgcg ggcttgttac ataatctccg
     1201 gctttcagct ccacggatcc acctgcctcg aaatccaaag tggcatgacc gcgaagcaca
     1261 atcacccatt cgtcctccgg ctgatcgtac cagaaatctg gcgggctaca gtgggagtgc
     1321 gacacgatgc gctcgatgtt cgtagagcgg ctctgaaaaa gcctaaaaaa attttccgca
     1381 ccaatctgct gaggcaaatc ggcaaaaata ttttttacct cgacgaacac aatcagcctt
     1441 tcaccagccg agcgcgcttg atcgccggga cgtcgatgat gcgccgttga ccgcgtatcg
     1501 gcggattctc cgcagcggaa atcatcgcgg tcatgaactg ttcgatcgtc actaagccca
     1561 atctttcggc ggtagagcga gtcccgggaa ataactcggc aagtttgtag agcggcacaa
     1621 tgagtttagg ccactgccgg ccgggtccaa gcacgtacca gggtcgcaga atgcttgccg
     1681 taattccggc ctcgcgaatc atggtttcgc cgagcgtacg cacccagagg taggcttgca
     1741 tcaccggcgc aggatgggcg acgctgacgt aaacaagatg atcgatgccc acgtgacggg
     1801 ccgcattgac gctcgcccgg atggaaatga gatcgatttt ttcgaactga tcggctttgc
     1861 tgggggttgg atgcggcgtc ccaaccaaat gaatcagcgt atcgcccgtg tgaagtgcag
     1921 ttgcgaccga tcgatcgtcg agcgcgtcac cgatgatagc ggccgcgcct ggggagacac
     1981 gcgcaatgga gtcctcgcgc gcgagcacgc gcacacgatg accacgcgcg atcaatggcg
     2041 gaatgagatt gcggccgatg tacccggtcc cgccggtgac cagtatatcg agcggagtgg
     2101 ccatgatcag aagtgcaaat caacctgacg atttcgttca agtcaattcg gcagccccgg
     2161 attctgcggc ttgtagcttg gatcgtcgtt acgccgccgc tgcaccgact gatcgacgat
     2221 ttcattgccg aactgggtcg tgccgatgcc gcggtaatgg cgcggctttt tctgcgcata
     2281 aaggccgctt aggaattgcg cgtacgagtc ggtgagcacg ccgcgatagt tttccgttcc
     2341 gatcccgact gggttcagcg ccagtcctac catgctggct ttctcctgga tccagcgaag
     2401 cgtcaggtcc gagagccgcc ggtcgggata gccgccgccc acatcgcagt gcgcgccgat
     2461 gaaccaacgc tgttccaatt tttgttgcgc cggcgcatcg ggattccaca aacaaacctg
     2521 gtaatcgata cgatgttcgt caaccgcaat ggcgtgaaac gcattctcga cgatgccgct
     2581 cagattggta tcgtgaaact cgtagaattt catgttgaca tcttgaagca catgcaacgg
     2641 aacgccgaga gcgccgacgg tatcccacac accgaggaat ttgatcttga tctcgcatga
     2701 aaaagcggca cggaatactt tagccgtgaa cgaatcgacg ctatcgccgc gcgtgcggta
     2761 aataccgtag gccatcggaa cccgtagcgt gagatgcttc ggtaggatca agccacaatt
     2821 gcggatcatc cctaccagac ttcgcgccgt gtaggcgccg cgactgatcc cgagcacgta
     2881 aacctcgtcc ccctcttggt acgctttgcc caggaattca tagccctgca cgatgttcaa
     2941 ttcgagaccg gcgccgatgc cgccgccgat gaagcggtcg taccactgcg tacccacgcc
     3001 ttcgtcgtac cattttagtt gcttgatccc gtccgcaccg ctttctttca ccgaggtata
     3061 aaaacggcag acattggttt ccacctgtcg acccggcggc aatttttcgt ccgccggccg
     3121 gttccatgtt ccatcgaaac agatcacaat tcgtttcatg cgttctcccc cgttagtaat
     3181 tgggaatgac ggcaaaatta cattgtaagc cattccgatt tgtttaggag agatttttgc
     3241 tgatagaggt gtttgaaatc cacgctttaa atttgccgag gctcagagca gcgagtctga
     3301 gatccgtatc ctccgttcaa tcgaaccact gtttttgcga caaaatatat tccgcagttc
     3361 tgtatgtgag cgccgccacg gttgcggtcg gcggataccc cgccatagtg ggaaaaaccg
     3421 aagatccgac gatgaagaga ttgggcacat catgagtctg acaaaattca ttgaccactg
     3481 aattatttgg atcggcaccc atcctcgttc cgcccgccca gcgattgggc cggccttttt
     3541 cttcggtcca cactttgctc gcgcccgtgg cgcgcataat tttttctccg acgcccgcca
     3601 tgaatttttg caaccgccgc tcgttctggt gaaaatcgaa ggtcacgcgc ggcagcggca
     3661 cgccccagcg gtcgcgatga tgcggatcaa gatcgacgcg attgcctttg tgcggcagcg
     3721 tttccggttg catgttgaga tccatgatcc gggtgtagta gcgcatatag aagtccttga
     3781 actctcttcc ccaccgcctc acgtccggcg ggagcacatc gaatctcgta atcggcgtgc
     3841 cgtcgccgct cgtgccaatc gttccgccgc ggataaaccc taagccggtg tgatcaaaat
     3901 tgttgccgtt gaagtcgtcg atacgcatcg ccgcggagcc cgggccgatg aaaccattga
     3961 tggtgtaatc gtcgaaggcg cccatggcgc gcacgtcgcc atgagccata atatttcgtc
     4021 cgacgtagcc gctactgttc gcaagtcctc tggcgaagcg gccccggccc gttttggaca
     4081 agagcagcaa acgcacatgc tcgaagacaa aggcgctcaa gatcaccacg cgggcacgca
     4141 gttcacgcgc tcgtcctgcc gcatcgaagt agctcacgcc tttgacacgc tcatctccgt
     4201 cggtattgat gcaaaacact ttcgagcggg ttctcagatc gaaattgccg gtttcgagcg
     4261 ccaccggaat cgtcgtataa agcgtcgcgg ccttggcatg aacgtggcag ccatagaaat
     4321 tgcaatgacc acaatagaca caggcgggcc gttccggaat ccgcgagtcg aacggctccg
     4381 atggacgata gggccgggac agaattcctg ctgcagtatc gtagggatga tagccaagct
     4441 tcttgcatgc ttcggcgaag aggttcatgc gcgcgctatg gcgcaacggc ggcagcggca
     4501 agccccgttt gcgcggaccg gcgaatgggt tggcgcccgc ctcacccgaa ataccgaact
     4561 cccactcgaa gcgctcgtag taaggctcga gatcgtcgta gctcagcggc caatcgataa
     4621 tgtcgtagcc ggtaagattg gcgcccgccc gctccgcatt gccgctttcg atctcatttg
     4681 tgaagagtct aaagtccccg ggcatgtagc gtgaggattg gccggtccag tgcaacaatg
     4741 cgccgccgag aacattgagc ggactcgtgc ggtattggag ccggctctta tcgccggttt
     4801 tgtttctggt cgtcgtgggt tcgtgacgca cccattccag ctgctcggga cgaatcagga
     4861 agcggatcga atcgcgcggc gcatagtctt cgagcttcgg cgccgggccg cgctcgaagc
     4921 caacgacttt caatccagcg gaggcgagtt gtttcgccat gatgccgccc agcccaccaa
     4981 ctccgacaat gcatacgtcg catccatcgg agttcgatcg cgccactttt agttcccctg
     5041 ctctttgggc gaatccactg ccacgggctc tagatctacg ggtttgttga tgtacgcatc
     5101 ggcgtaaccg aattgctgac cgggaaaatt gacgagccgc cagccgatca tgtcgcgatt
     5161 accgccatac tgtggctcgc aaaaaacacc ttccagaaca tggcagcgca cggtttcaaa
     5221 aaaatccgcg gccggtttga agccgggcac cgcgccactt tcgaagtcga ccagcacttg
     5281 atccttttga gcctcactga gcagacaaaa tgcccggcgg aactttgcct tcgcgcatcg
     5341 ctcgatggcg cgcaagcctt ggcgataaaa cggcgcgaaa gctgcgtaat caccggcaag
     5401 tgcgacttcg atgtactgaa ccgcgccgat ctccaccgcc ccggccgttt ccgtccgggg
     5461 aaaaatccga tccgctattg ccgccaggat cttactttcg tcagccgtta aaacattgtc
     5521 gcaatggttc atcgaatcca tatcgatcag ctccgtgaaa tgttttcatg aatagcagag
     5581 tcggcgcccc gatagccagt gaacacgctt agaagcgcac aactcggcaa ggattaggta
     5641 atggaagcgt cgaatttctt tccaggcggt gcggcgagaa ttgttttgaa tcggtcaagc
     5701 agttgcatga cctgattttc tatcatcagc aaagcacggt gcttcggttt gacaaaatcc
     5761 tgcgccaccg cgtgatcgag aaaattgatc agtgaatcat aatatccgtc gacgttcaga
     5821 aggccgcatg gtttgttgtg tagaccgagt tgggcccagc tcagcacctc gaaaaactct
     5881 tccatcgtgc caatgccacc gggaagcgcc acaaagcccg cggccaactc ggccatcagg
     5941 gccttgcgct cgtgcatgga ctgaaccacg cgcaactccg acaatccggt atgcgcaact
     6001 tctttggtaa ccaaggcgtc cggaatgact ccgatcacat aaccgccagc ctccaaggct
     6061 gtgttggcca gaaccttcat cagtccgaca ttcccgccgc cgtaaacgac gccgaaaccg
     6121 cgcttgacca actcgccagc gcattccgcc gctgcctgag cgtattcggg cgatcctcca
     6181 tgccgggaac ctgtgaatac gcagatgctc cgttctggac gtttcgtcat catttctact
     6241 cgatcggaaa ctcggccggt aaaacagttt aagccgtgga agtgcaaccg cgtcgaagga
     6301 tttccaatgc gttatcggcc tccggcgcga tcacggccgt tatcttcata tggctttgca
     6361 aacgcgtgtt ggcggtcacc acctgctcgg tgtgatccag cgcgcagcgc accagaatgc
     6421 agccggacgg caacccgagt tcgcgcacca accggccagc aaacggcgca tccagttcga
     6481 tttcgaattc ggcgacaatc ggctgcgcgc gctcattgcc aaatccgccg cgctccaggt
     6541 cgcgctcgag tagtgcttca taaatcggca aatcgcggag atactccgcc acggcgtacg
     6601 cacaaaaact actcgtcagc aacggtaaca tctgttcgta gctgccggtc atttcggtga
     6661 tcaacagaat cccggtgagc ggggcgcgca caatggcggt aaagtacgcg gccatgccga
     6721 ccaccgcaaa cacgccgggc tccggcgcca ggacgggcat taacccatga gcgagatgac
     6781 cgacgccgag accgagcaac gcaccgagaa ccaacaacgg cgaaaaaatg ccgcctgccg
     6841 agcctgttcc gtagctcgcc accgtgagcg caaagcgcag caaaaataaa atcgggacgg
     6901 cgatgatgcc aatccgattc gtgagtacca tttcccccaa ctcgtgcccg ccgccggcag
     6961 cgaccgggta gaaccacgcc accaagcccg ccgcaactcc tacgctagcg gccaacgcga
     7021 cacgctgccg gccattgaac cgtgccgcaa aatcaactcc ctcgaggagc gatcgattga
     7081 atgctacacc gaatccgccc gcggcgattc caagcacggc aaaagccggc agtgcgagta
     7141 acgacggcat tgcgtaattc ggcacggtaa ataccggcaa ctgtccggat accgagcgcg
     7201 cgaccacatc cgcgctcgcc gccgccaaaa aagccgcgcc aaaaaccgct ggccgaaaat
     7261 cgcgccgcat ttcctccagt acaaaaacca ggcctgacaa cggcgcattg aatgccgcgg
     7321 caaggccggc gcccgcgccg gcagccgtca gggtcaaccg gtcggctgaa gaaactttca
     7381 tgctgcgcgc gaggccgtcc gctactgcac cgcccatttg cacggtcgga ccctcgcgtc
     7441 cgagcgcaag cccgctgccg atcgcgagca cgccgctgat cattttgacg accaacacac
     7501 gcatccagga gagatcgcgc aatcggtgca aaacggcttt cagatggggg atgccgctgc
     7561 cgctagtttc gggcgcgtag cttactacca ggcaaaccgc cgccgcagcg ccggcggcac
     7621 cgaagaccag aggaaaaatc cagccgaatg ccggcgtcga gtacgaccag tggaccagac
     7681 cgtttcggag cgcatcgcca gccgctagta aggcgcgaaa gacgaccgcg acgcctcccg
     7741 ccgcaagacc aacggcagcc gcccgcggaa acagcttatg acgctgctgg ctgaccgcca
     7801 gataatcttg aacttccgat tttgcttcta tgaaagcgct ggccggtttc gcttctccct
     7861 gtggttcggt ttccatagtc taataaacag ccatcaggac taattgcagg ttcactgcga
     7921 ctcgagctta tcgagtacga acaaaaactg gtcgagatcg gcgaaatccg gtaaaacgta
     7981 agacgcccca gcggcgagaa gcttctctct ttgtgcgccg gcgccgatgc caagaaacgg
     8041 tatgccgagg agtcgggaca tttttacatc ccagactcca tcacccacgt aaatcgtccg
     8101 attccattct ttccatgtct caccggccgt cctggccgcg gcgattcgaa caatggtttc
     8161 acggtccggc gcgtcgtccg ctgaagcctc gggataatcg tcgtagcaaa acccggcgcc
     8221 tgccattttg atacgcgctg catcggacca cgatccggtc gccaagccga tacgatactc
     8281 gccgcgttcc ctcagtcggg ttagtaaatc cagagcgccg cggatcggag taaacgggcc
     8341 ttgcagcgcg gccgagcgaa acaacgcgac aagatagtct ctaaactgga ctgtttctga
     8401 agcagtcgga ctacgcccac ggcgagattc gaaaacctcc tgaaaaatcc ccgcgtcggt
     8461 cgtatttctg tagcgcgacc aatctgggtc gacatcggca aaaccgcaga catcgacaaa
     8521 ggcttgcaaa aagcatcgtg tgtccacgtc catggtgtcc gtcaatgttc cgtctatatc
     8581 gaagaggatt gtttccataa caaccgagag gtgcaagctc gggagagaaa aggcgggttc
     8641 aaaagacacg cccgagaaat ttgcttctcg ccgccggatc ccaattctga tatggccatg
     8701 cttgtcatct cgcgttctca cgacatacga agaaagattt ctcgcctggg ctcgacatga
     8761 cagctaagaa accgtcgcaa gacggaaaat taactacgct gacgaagaaa acaatacgcc
     8821 tcacttccac aatttctgga taaacccttc cttatcaagc tcctgaaaaa atcggccatc
     8881 gatgaactgt tcgggattga gattagccac tcgcggatcg gtttcggcca tctggtcgag
     8941 tacggtttta agcgactccg gcgaaggccg cggaattcgc
//
LOCUS       DASULZ010000650         7140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_581584, whole genome shotgun sequence.
ACCESSION   DASULZ010000650 DASULZ010000000
VERSION     DASULZ010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7140
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581584"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VFY96_13500"
     CDS             complement(<1..738)
                     /locus_tag="VFY96_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020646777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5607533.1"
                     /translation="MDRQVVVITGASAGVGRATARAFGERRARVGLLARGRDGLEAAK
                     REIERSGGEGFVFPLDVANPEQVEAAAQAVEENLGPIDVWINNAMTSVFSPVKEMNAE
                     EFKRVTEVTYLGYVYGTLSALKRMLPRDRGVIVQVGSALAYRGIPLQAAYCGAKHAVQ
                     GFMDSLRCELLHDHSNVRVAMVQMPALNTPQFSWVKNKLPNKSQPPGPIYQPEVAAKA
                     IVWASENPRRELNVGFGTTQAIVANSFV"
     gene            complement(731..1666)
                     /locus_tag="VFY96_13505"
     CDS             complement(731..1666)
                     /locus_tag="VFY96_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019509389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX5607534.1"
                     /translation="MAAESTRVIYAAIVANLAIAVTKFIAAGFTGSSAMLSEGIHSLV
                     DTGNGGLMLLGVRRSRRPADAEHPFGYGKELYFWTLIVAIVIFAVGGGISAYEGLLHI
                     LNPKPIADPLWNYIVLGIAMVFEGYSFTVAFKAFEAERGKLSIWQSIEQSKDPTTYTV
                     LFEDSAALIGLVVALIGVFLAHFFENPYFDGAASIVIGMILAAVAVLLAYESKGLLVG
                     EAVDAETLKDIRRLAESDPRVESVKNALTMYFGPHTILLAMDLSFRPNLSAIEVEESI
                     DRLEENVRSRYPNVQHIFIESESLTPASKRRVAHG"
     gene            complement(1766..2143)
                     /locus_tag="VFY96_13510"
     CDS             complement(1766..2143)
                     /locus_tag="VFY96_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015156223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HEX5607535.1"
                     /translation="MTNVGAKRWAIAEGYIPAYSHGEAPQLISHETVCILNAGDTDAE
                     VTITIFYTDREPGGPYRLKVPARRTQHVRFNNLDDPEPIPKDTDFASVIEASVPIVVQ
                     HTRLDSRQAETALLSTVAYAAGD"
     gene            complement(2181..3137)
                     /locus_tag="VFY96_13515"
     CDS             complement(2181..3137)
                     /locus_tag="VFY96_13515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008424305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX5607536.1"
                     /translation="MKIGYSLSSEETTPADLVRYAVRAEQAGFSFALISDHFHPWLTR
                     QGQSPFVWAVIGAIAQATKQITLGTGVTCPIMRIHPAIIAQAAATAALLMPGRFFLGL
                     GTGENLNEHILGMHWPPSDVRRKMLQEAVMILRLLWRGGPQSFRGEYFTVEKTQVFSL
                     PEKPPPIMIAAAGGRAAELAGEIGDGLISTMANDSLVRKFRVSDGASDKPRYGQMTVC
                     WAKTEDEAKKIAQEWWAVAAIPGALMSELATPAEFEAVAQLVNEESVAAKIICGPDPE
                     RHREEIESYRKAGFDHVYIHQVGPDQNGFFEFYQREILPKFK"
     gene            3874..4638
                     /locus_tag="VFY96_13520"
     CDS             3874..4638
                     /locus_tag="VFY96_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006346832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607537.1"
                     /translation="MADRALTLEQFKTLRKDNWWLQPLLVVVGLGGFAIYATWAALQN
                     AHYFVDPYLSPFYSPCLATNCTHVSLPVIGSWWNLSPALLILWIPGGFRATCYYYRKA
                     YYRSFFLSPPACAVRDASRSYTGETRFPFLLQNLHRYFFYLSLVILAFLWWDALVAFR
                     FPDGFGVGLGTIVLCVNAALLSLFSLSCNSCRHVCGGYLNSFHRAPSKYKLWSFITGL
                     NEKHMQYAWVSLVFVALADLYVRLLSMGVISDLRFL"
     gene            4638..6440
                     /locus_tag="VFY96_13525"
     CDS             4638..6440
                     /locus_tag="VFY96_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEX5607538.1"
                     /translation="MAEDKYETHEYDVVVIGAGGAGLRAAVEASAQGVKTALVCKSLL
                     GKAHTVMAEGGIAAAMGNVYPEDNWRVHFRDTMRGGKLLNNWRMAQLHAQEAPDRVLE
                     LEEWGALFDRTKEGLILQRDFGGHRYARLAHVGDRTGLEMIRTLQQHAVHKGIDVYME
                     CNLQRLLTDGGRVSGAFGYWRESGKFILFKCKAVVLATGGGGKAWKVTSNSWEYTGDG
                     VTMALDAGADLMDMEFMQFHPTGMVWPPSVRGILVTEGVRGDGGTLKNTEGKRFMFHY
                     IPEFFKAETADTEKEADAWYEDKRNNRRTPDLLPRDEVARAINSEVKAGRGTPHGGVF
                     LDIASRRPADYIQRRLPSMYHQFRELADVDITKEPMEVGPTCHYAMGGVRVDADSTAT
                     TVPGLFAAGEVAAGLHGANRLGGNSLSDLLVFGRRAGLYAAEYAKSFAGAVSINNDQL
                     DQYAHDLLAPFEIHGKENPYTIHSDLQQSMQELVGIIRVESELNQALEIIQRLRQRSR
                     QVKVEGNRQYNPGWHLALDLHSLLTFAEAATLAAIERKESRGGHTRDDYPNTDAKFAR
                     VNVVIRKRDGQMTLAQEPLPDMPGELKALLEEKS"
     gene            6440..>7140
                     /locus_tag="VFY96_13530"
     CDS             6440..>7140
                     /locus_tag="VFY96_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEX5607539.1"
                     /translation="MPDQQVHMRVWRGDASGGEFKDYRIAVGEGQVVLDVIHAIQAQQ
                     ANDLAVRWNCKAGKCGSCSAEVNGKPRLMCMTRMNLFQPGETITVSPMRTFPVIRDLV
                     TDVSFNFRKAREVPAFKPKPKEPDGTRRMFQEDIDRVQEFHKCIECFLCQNVCHVIRD
                     HDENKPAFAGPRLFVRLAALEMHPLDTNDRMELIRQKAGIGYCNITKCCTEVCPEHIH
                     ITDNAIIPLKERVVDR"
BASE COUNT         1566 a         2000 c         1974 g         1600 t
ORIGIN      
        1 cacgaagctg ttcgcgacga tggcctgggt cgtcccgaac ccgacgttca actcgcgccg
       61 cggattctcc gacgcccaga cgatcgcctt cgcggcaact tccggttggt agatcgggcc
      121 cggcggctgc gacttgttgg gcaatttgtt ctttacccag gaaaactgcg gcgtgttgag
      181 ggcgggcatc tggaccatcg caacacgaac attgctgtgg tcgtggagca gctcacagcg
      241 aagagaatcc ataaaccctt gaaccgcatg cttggcaccg cagtaggcgg cttgcagcgg
      301 aataccgcga taggccagcg cggaaccgac ctgcacgatc acgccgcgat cgcgcggcag
      361 catccgtttc aaggccgaca aagtgccata cacataacca agataagtga cctcggttac
      421 ccgcttgaac tcttccgcgt tcatctcctt cactggcgaa aacaccgaag tcatggcgtt
      481 attgatccag acatcgatgg gtccaagatt ttcttccacc gcttgcgccg ccgcctcgac
      541 ctgctctgga ttcgccacgt cgagcggaaa aacgaatcct tcgcctcccg atctctcgat
      601 ctctcgtttc gccgcttcca atccgtcccg cccgcgggca agtagcccta ctcgcgcccg
      661 gcgttcgccg aacgcgcgcg ccgtagcgcg gcccaccccg gcggaagcgc cggtaatcac
      721 gaccacttgt ctatccatgg gcgactctcc tcttggacgc cggcgttaac gactccgatt
      781 cgataaaaat atgctgcacg tttgggtacc gactgcgaac attctcctca agtcgatcga
      841 tactctcttc gacttcgata gccgaaagat tcggtcgaaa gctcaaatcc attgcgagta
      901 ggatggtatg cggaccgaaa tacatcgtca gggcgttttt cacgctctcg acccgcggat
      961 cagattcggc gagacggcga atatctttta acgtctcggc atcgacggct tctccgacga
     1021 gcagaccttt gctttcgtag gcgagcaaaa ccgctacggc cgcgagaatc atgccgatga
     1081 caatggatgc agcgccgtcg aagtaaggat tctcgaaaaa atgcgcaaga aagacgccga
     1141 tcaatgccac caccaatccg atcaaagcgg cggagtcttc gaacagcacc gtgtaagtcg
     1201 tcgggtcttt actttgctcg atggactgcc agatactcag tttgccgcgc tcagcttcga
     1261 atgctttgaa tgccaccgtg aacgaataac cttcgaacac catggcaata ccgagaacga
     1321 tgtaattcca cagcggatca gcaatcggtt tggggttgag aatatggaga agaccctcat
     1381 aggccgatat gccgccgccc accgcgaaaa tcacgatcgc gacaatcaac gtccagaagt
     1441 aaagctcctt gccatagccg aaggggtgct ccgcatcagc gggtcgtcgg ctgcggcgaa
     1501 cgcccaaaag cattaagccg ccgttgccag tatcgacgag cgagtgaatc ccttcggaga
     1561 gcatggccga actgcccgta aaacccgcgg caatgaactt ggtcacagcg atggcgaggt
     1621 ttgcaacgat ggccgcgtag atgactctgg tcgattcagc cgccattttt attggagtgg
     1681 atcgatcaac gcgaggcaga gtccgttgga attttgtaag cagaaacttc gattcgtttt
     1741 atttctggat ctctggacat gccgctcaat cgccggcggc gtatgcgacc gtgctgagca
     1801 aagcggtctc agcctgacga gaatcgagcc gggtatgctg gacaacgata ggcacactgg
     1861 cttcgataac actggcgaaa tccgtgtctt tcggaatagg ttcgggatca tcgagattgt
     1921 tgaagcgcac gtgctgcgtc cgacgcgccg ggaccttcag gcgataaggg cctccgggct
     1981 cacggtcggt atagaagatc gtgatggtga cttcggcgtc agtgtcgccc gcattgagga
     2041 tgcaaacagt ctcatggcta attagctgag gcgcctcgcc gtggctatag gccgggatat
     2101 aaccttctgc gattgcccat cttttagcgc caacgtttgt catagtgttc gccccgccgc
     2161 tgtttgcggt tcaatcggca ctatttgaat ttcggcaata tctcccgttg ataaaattcg
     2221 aaaaacccgt tttgatccgg gccgacctgg tgtatgtaaa catgatcgaa tcctgctttg
     2281 cgataggatt cgatctcttc gcggtgtctt tcaggatccg gtccgcagat aatctttgca
     2341 gccaccgact cctcgttcac gagctgggcg acggcctcga actcggccgg ggtggcaagc
     2401 tcgctcatta atgcgccagg gatcgcagcc acggcccacc actcttgggc gatctttttt
     2461 gcctcgtctt ccgttttggc ccagcatacc gtcatctgac cgtagcgcgg cttatcgctg
     2521 gcgccgtcgg aaacccggaa tttccgcaca agagagtcgt ttgccatagt gctgatcaac
     2581 ccatcgccga tttcgccagc gagctcggct gccctgcccc ccgcagcagc aatcatgatt
     2641 ggcggcggtt tctccggcaa actgaaaacc tgtgtcttct caacggtgaa atattctccc
     2701 cgaaagcttt gcggaccgcc gcgccagagg agccgcaaga tcatcaccgc ttcttggagc
     2761 atcttccgcc gtacatcgct cgggggccaa tgcatgccga gaatatgctc gttcaagttt
     2821 tccccagtgc cgagacccag aaaaaaccgg ccgggcataa gaagtgccgc ggtcgccgcc
     2881 gcttgagcaa taatcgccgg atgaatgcgc atgatcggac aagtgacgcc agtgccgagc
     2941 gtgatctgtt tcgtggcttg cgcaatcgca ccgatcaccg cccaaacgaa cggactttga
     3001 ccttgtcgcg tgagccacgg atgaaaatga tcggagatta acgcgaagct gaagcctgct
     3061 tgttcggccc gtacggcgta gcggactaaa tccgcaggag tggtttcttc acttgaaagt
     3121 gaatagccga ttttcattgc atgcctccgg caggtccggc ttcgaccgtt tcggaatatc
     3181 cctgggtcga ggaatcgctc caactgggca tcaacagatt agatgcctgc gacaccggcc
     3241 gccctcgtgc cgttactcgc tttttcagaa ggtgataaat gctgtcgttt tttactggtt
     3301 gcacgtaagt atcggctgat tgcatcaggc aaaggggccg caatagacat gccaccgtcc
     3361 gcgagctagt tgaagctcct tttgcttctc aagaacgcta cactgctgca tccttcgttg
     3421 cacatctgag acactgcttc gcctgcccat ccaaatctcc gcttggccct acgtcaatag
     3481 aaaccgacac tgatatgatc cggatcaagg gttttgccga cgtgcaaacg atttccgttt
     3541 ctgctataaa aaaattgaat agcctatgcg aacggagctc gtgtactaca gctgcatctc
     3601 agtgaaaatc atgtttgaaa aataaccgga gcagaccagc ccatcctgtc gacactgcgg
     3661 cttgggacaa agccgcaaga agcgcaatca aggcaaaagt tcgcggcggc tgcagtggct
     3721 ggaagtatcc cgcctcgaaa tagcggtgcg ttctgagtgg aggctgcggg ggagatggac
     3781 agcggatgat tgcttgacac tgaaatccca tctgttttta tcaaaacgga gagcaataat
     3841 gacgctatta tcaggagcaa aaggaaaacc gtcgtggcag atcgagcgtt gactttggag
     3901 caatttaaga cgttacgcaa agacaattgg tggcttcaac cattacttgt ggttgtcgga
     3961 cttggcggct ttgccattta tgcaacgtgg gcggctctgc aaaacgccca ctattttgtc
     4021 gacccctatc tctccccgtt ttactcacct tgtctcgcga ctaattgtac gcacgtcagc
     4081 ttgccggtta tcggttcctg gtggaatctt tcgccggctc ttttgatttt atggatacca
     4141 ggcggttttc gcgcgacctg ctattactac cgcaaagctt actatcgctc tttctttctc
     4201 tcacctcccg catgcgccgt gcgcgatgcg tcccgcagct atacgggtga aacgcggttt
     4261 ccttttttgc tgcagaacct gcatcggtac tttttttatc tttcgttggt cattctcgcc
     4321 tttctttggt gggatgcctt ggtagcgttt cgttttccgg acggatttgg cgtcggcctc
     4381 ggcaccatcg tgctctgcgt taacgccgcg ctgctatcgc ttttcagtct gtcctgcaac
     4441 tcttgccgcc acgtttgcgg tggttatttg aactcctttc atcgcgcccc gagcaagtat
     4501 aagctctgga gcttcatcac cgggctaaac gaaaaacata tgcaatatgc ctgggtgagt
     4561 ctggtctttg ttgcgctggc cgatctttac gtccggttgc tatccatggg cgtgatctcc
     4621 gatttgaggt tcttgtaatg gccgaagata aatacgagac ccacgagtac gacgtggtcg
     4681 taatcggcgc cggcggcgcc ggtttgcgcg ctgcggttga agcgtcagcg cagggcgtaa
     4741 aaacagcgct ggtgtgcaag tcgctgctcg gtaaagcgca taccgtgatg gccgaaggcg
     4801 gcatcgcagc cgccatgggt aatgtctacc cagaggacaa ttggcgggtt cattttcgcg
     4861 acaccatgcg cggcggcaag ctgctcaaca attggcgcat ggctcagctt catgcgcagg
     4921 aggcgcctga tcgcgtgctc gagctcgaag aatggggagc gctgttcgac cgcaccaaag
     4981 agggactcat tttacagcgc gatttcggcg gccaccgtta tgcgcggctg gctcacgttg
     5041 gcgatcgcac gggcctggag atgatccgca cgcttcagca gcatgccgtt cacaaaggca
     5101 tcgatgtcta catggagtgt aatcttcaac gcttgctcac cgacggtggt cgagtcagcg
     5161 gtgcctttgg ctactggcgc gaaagcggaa aatttatctt gttcaaatgc aaggcggtgg
     5221 tgctcgccac gggcggcggc ggcaaagcct ggaaggtgac ttccaactcg tgggagtaca
     5281 ccggagacgg cgtcaccatg gcactggatg ccggcgccga ccttatggat atggaattca
     5341 tgcaattcca tccaaccggc atggtttggc cgccgagcgt gcgcggcatc ctggtgacgg
     5401 aaggcgtccg cggcgatggc gggacgttga agaataccga aggcaagcgc ttcatgttcc
     5461 attacattcc agagtttttc aaagccgaaa cggccgacac cgaaaaggaa gccgatgcct
     5521 ggtatgagga caagcgcaat aatcgacgga ctccggattt gctgcctcgc gatgaagtcg
     5581 cgagggcaat taattcggaa gttaaagccg ggcgcggcac accgcatgga ggtgtttttc
     5641 ttgatatcgc atcccgccga ccggcagact acattcagcg caggctgcct tcgatgtatc
     5701 atcaattcag ggaactggcc gatgtggata tcaccaaaga accgatggaa gtcggcccca
     5761 cctgccatta tgcgatgggc ggcgtccgag tcgatgccga tagcacggca acgacggtgc
     5821 cgggactatt tgccgccggc gaagtcgccg cggggctgca cggcgccaat cggctcggtg
     5881 gcaactcgct ttcggatctc ttggtgttcg gccgtcgcgc cggactttat gccgcggaat
     5941 acgcgaaatc ttttgccggc gcggtttcga tcaataatga tcagctcgat caatacgccc
     6001 acgatctcct ggcacccttt gagatccatg gcaaggagaa cccgtacacc atccactccg
     6061 atctgcaaca atccatgcag gagctcgtcg gcatcattcg ggtcgagtcc gagttgaatc
     6121 aagctttaga aatcattcaa aggctgcggc aacggagccg gcaagtgaag gtcgagggca
     6181 accggcaata caatccggga tggcatctcg cgctcgattt gcactcgctt ctgacttttg
     6241 cggaagccgc cactttggcg gccatcgagc ggaaagagag ccgcggcggt catactcgcg
     6301 acgattatcc caatacggat gcgaagttcg ccagagtcaa tgttgtgatc cgtaaaagag
     6361 atgggcaaat gacacttgct caggagcccc tgccggacat gcccggggag ttgaaagcat
     6421 tgctcgagga gaaaagttga tgcctgatca gcaagtccat atgcgcgtat ggcgcggtga
     6481 cgccagcgga ggtgagttca aggattatcg aatcgccgtc ggcgaagggc aggtggttct
     6541 tgatgtgatc cacgccatac aagcacagca agctaatgat ctagcggttc gctggaactg
     6601 caaagccggc aaatgcggat cgtgcagtgc cgaggtaaac ggcaaaccgc ggctcatgtg
     6661 tatgacgcgc atgaatctgt tccagcccgg cgagaccatc acggtttcac ccatgcgaac
     6721 ctttccggtg attcgcgacc tcgtgaccga cgtctcgttc aatttccgaa aagctcgtga
     6781 ggtgcccgcg ttcaaaccga aacctaaaga gcccgacggc acccgtcgca tgtttcagga
     6841 agacatcgac cgcgtgcaag agtttcacaa gtgtatcgag tgttttcttt gccagaacgt
     6901 ttgccacgtg attcgtgacc acgacgaaaa taagccggcc tttgcaggtc cacgcctgtt
     6961 cgttcgattg gcggctctgg aaatgcaccc gctcgacacc aacgatcgca tggagctcat
     7021 tcggcagaaa gcgggaatcg gctattgcaa cattaccaaa tgctgcaccg aggtttgtcc
     7081 cgagcatatt cacatcaccg ataatgcgat tatcccgctg aaagagcggg tggtggaccg
//
LOCUS       DASULZ010000651         5000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_682777, whole genome shotgun sequence.
ACCESSION   DASULZ010000651 DASULZ010000000
VERSION     DASULZ010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682777"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..950
                     /locus_tag="VFY96_13535"
     CDS             39..950
                     /locus_tag="VFY96_13535"
                     /inference="COORDINATES: protein motif:HMM:NF024592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HEX5607540.1"
                     /translation="MAATAAVITTIVLSLKPFLHRWLQRIDERELTAALKLLLISVVI
                     LPVLPDRGYGPWQALNPYELWWLVVLIALISFAAYCAVKIAGTERGILLAGFLGGLVS
                     STGVTLHLSHLAIEGAERSIIAAGILMASATMFMRVLFLAALLNPALAKLLVPSMSVM
                     AIWLFIAAVLRTRKSERPAVNSLNLRNPVELGQALQFGLLLVLIILASHAIRDWLGEA
                     GIYLLAAISGLADVDAITISMARLGVGEISMHSAALAVLAATVVNTLTKATLVAIIAG
                     SKTAAQIVGSIVGCVLIGVAVGWWWLY"
     gene            complement(973..1101)
                     /locus_tag="VFY96_13540"
                     /pseudo
     CDS             complement(973..1101)
                     /locus_tag="VFY96_13540"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
     gene            complement(1204..1797)
                     /locus_tag="VFY96_13545"
     CDS             complement(1204..1797)
                     /locus_tag="VFY96_13545"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5607541.1"
                     /translation="MLSSSRPIFSLASEVACGHALKIALQSKAKLDIMHVESHVKQEK
                     ACWLDFPAVRATLTRWDILPNRVRQEEVAKAGLRIRKILSSSGDPVESMLRHFHRFPP
                     DLIVLATHQRKGLDRWLHKAVAEPLARRSGAMTLFIPHEGRGFVSPSDGSVALEKILV
                     PVDHDPDPQAALDRALLLALGLNHARRISPNAHCKRQ"
     gene            complement(1924..2412)
                     /locus_tag="VFY96_13550"
     CDS             complement(1924..2412)
                     /locus_tag="VFY96_13550"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5607542.1"
                     /translation="MGLDFKKILCPVDLSRFSLDALRLAVNVADASGATLDILHVIFD
                     DIYLTEITQTDPALIDAYATEPQRRAEIVRATEERLEVFLKQFCHDVVQHHGNVRYHV
                     RRGDPFEEILETTEDLMTDLVVLATHGRTGIKRLVIGSVAEKIVRHARCPVMTVKPKP
                     VS"
     gene            complement(2416..2859)
                     /locus_tag="VFY96_13555"
     CDS             complement(2416..2859)
                     /locus_tag="VFY96_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607543.1"
                     /translation="MKKLCVLIILTAALLFNQNAMTESFRVVENLRHEQINLPPSIPD
                     RNRMVVIDYTMFVEDRGAAGILIFYDDGRTQREVDYIESYDLEGNLLLVSWIDHFGVC
                     QVAMDRGLIDAEEPEIAGTLVLIGGLGRELWLGACAIKFEKNREG"
     gene            complement(3360..4418)
                     /locus_tag="VFY96_13560"
     CDS             complement(3360..4418)
                     /locus_tag="VFY96_13560"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEX5607544.1"
                     /translation="MYCRVIACDFDGTGADDGHPAPELYAALSAARAQGIVTLLVTGR
                     VLEDVQNACGELSPFDAVVAENGALIYLCTLRRTIQLGEPPTEHFLGELRAHGVPFHT
                     GAVIVGTWEQHTSEVLELIRQCGIDGQLVFNRGALMVLPSGINKAVGIRRALDELGRS
                     ERNLIAFGDAENDIPMLVNAETGVAARGSVPAVMALVDGRLSQAGGAGVAVYIRKLLE
                     HGGVVPTPRRRAVVLGKNSGGHRVTLLTSGTNVVISGDPRSGKSWIAGLLAEQLVERG
                     YRICVIDPEGDYAQMGERTRVITFGDEIALPSPAAATRLLSNAALSIILLLSSLAPQQ
                     QRNYVDELLAALGGGTSK"
     gene            complement(4431..4916)
                     /locus_tag="VFY96_13565"
     CDS             complement(4431..4916)
                     /locus_tag="VFY96_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX5607545.1"
                     /translation="MPILKRLKEVLDNSKIPYEVYNHALAYTAQEIAAKQHFSGNEMA
                     KVVMLEVDDELVMGVIAGNHKINPETVRASLGAGRVRMATEDEFISRFPECEIGAMPP
                     FGNLFGLKVFVDPALAKDEYIYFNAGNHVQTVRLRYKDFTALVNPRIVALVNGHNKRA
                     A"
BASE COUNT         1112 a         1412 c         1285 g         1191 t
ORIGIN      
        1 tcacccttgg cgctctgtca gtccgcggcg agcacgctgt ggccgccact gccgcagtga
       61 tcaccacgat tgttctcagt cttaagcctt tcttacatcg ttggctgcaa cggattgacg
      121 agcgggaatt gaccgccgcc cttaaactgt tgctgatttc ggtcgtgatt ctgccggtgt
      181 tgcccgatcg aggttacggg ccgtggcaag cgttaaaccc ctatgagctt tggtggctcg
      241 tcgtcctgat cgctctgatc tcttttgccg cctactgcgc ggtcaaaatt gccggcacgg
      301 aacggggcat tttgctggcc ggtttcctcg gcggactagt ctcttcaacg ggagtcactc
      361 tccatctgtc gcaccttgcg atagaaggag ccgagcgcag tatcatcgct gctgggatat
      421 tgatggcgtc ggcgaccatg tttatgcgtg tgcttttttt agccgcatta ctaaaccccg
      481 ctttggcaaa attgcttgtt ccgtccatgt ccgtgatggc tatctggctt ttcatcgcag
      541 cggttctgcg aacacgaaag agtgagcgac ccgccgtcaa ttctttgaat ctacgcaacc
      601 cggttgagct tggacaggcg ttgcagttcg gtctgctact cgtccttatc attctggcta
      661 gtcatgccat ccgggattgg ctgggagaag ccggaatcta tttgcttgca gctatttccg
      721 gtcttgctga cgtggatgcg atcaccatat ctatggcgcg cttgggagtt ggtgaaatct
      781 cgatgcattc ggctgctctc gccgtcttgg ctgcaacggt ggtcaacaca ctaaccaaag
      841 ctactctggt ggcgatcatt gctggctcga aaacagccgc ccaaattgtc ggatcgatag
      901 ttggatgtgt gttgattggc gtggctgtcg gctggtggtg gctctactag ggtcgatgtt
      961 tagagcgctc gatcatctga ccggcacggc gagtaccggg caacgcgccc cgcgcagcac
     1021 tctttccgtg gtgctgccgc gcagggcgtc caaaaaaccc atatgccctt gggttgcgag
     1081 caccatgagt ttgggacgcc agtgtgtctc agcttgagga tttcgtccac gacctcacct
     1141 cgtcttacga ttgtttcaaa agaccatcca cgccccagag acaggtccac gacgggtggc
     1201 ttcctattgc cgcttgcaat gtgcattagg cgaaattcgc ctagcatggt tcaggcccag
     1261 cgccaaaagc aaagctctgt cgagtgccgc ttgtggatcg ggatcatgat caacaggaac
     1321 gagaattttc tcaagcgcga ctgaaccgtc actgggtgaa acaaatcctc tgccttcgtg
     1381 aggaataaac agcgtcatcg ctcccgaccg gcgcgcgagc ggctccgcga ccgctttatg
     1441 caaccaacga tcgaggcctt tcctctggtg cgtcgccaat acgattaagt ccggcggaaa
     1501 tctgtggaaa tgccgcagca tgctttcgac gggatcgcca cttgaagata gaatctttct
     1561 tatacggagt ccggctttgg cgacttcttc ctgccgaacc cggttaggca aaatatccca
     1621 gcgcgtcaat gtggctcgca ctgctgggaa atcaagccag caggctttct cttgcttaac
     1681 atgtgattcc acgtgcatga tatcgagctt cgccttcgac tgcagggcaa ttttcagggc
     1741 atgaccgcag gccacttcgc tagctaagga gaaaatcgga cggctggaag ataacaaact
     1801 ttcgactaac ctggttttga atcgaggcca tcggtctgaa ttcccattta ttaattgctt
     1861 ctttgtcttt caaacgagta aggcacggcc gtcagtcatt caggtcgagc gcccagtggc
     1921 aaattagctg acgggcttgg gtttcaccgt catcaccgga cagcgcgcat ggcgcacgat
     1981 cttctccgcc acgctgccga tcaccagccg ttttataccg gtccgcccat gcgtcgccaa
     2041 gaccacgaga tcggtcatca aatcctccgt ggtctcgagg atctcctcga aaggatcgcc
     2101 cctgcgcacg tgataacgta cgttgccgtg atgctgcacc acgtcgtgac agaactgttt
     2161 gaggaaaacc tccaatcgct cttcggtggc gcgaacgatc tccgcccgcc tttgcggctc
     2221 ggtcgcgtaa gcatcgatca gcgccggatc tgtttgggtg atctcggtca ggtagatgtc
     2281 atcaaaaatc acgtgaagaa tatcgagagt ggctccgctc gcatcggcga cgttcaccgc
     2341 taaccgcaaa gcatccaggg aaaatcgcga aagatccact gggcagagaa tttttttaaa
     2401 atcgagaccc atcgtttagc cttcccggtt tttctcaaac ttaattgcgc atgcgccaag
     2461 ccaaagttcg cgaccgaggc cgccgatcag caccaatgtt ccggcgattt ccggctcctc
     2521 agcgtcgatc aagccgcgat ccatcgcgac ctggcacaca ccgaaatgat cgatccacga
     2581 caccaatagc aggtttcctt cgagatcgta ggattcgata taatctacct cccgttgagt
     2641 gcgcccatcg tcatagaaaa tcaggattcc cgccgcgccg cgatcttcga caaacatcgt
     2701 gtaatcgatc actaccatgc ggtttctgtc cggaatcgaa ggcggcaaat taatctgttc
     2761 atgccggaga ttttcaacga ctctgaacga ttcggtcatc gcgttctggt tgaacaacag
     2821 agccgctgta agaattatca agacgcagag ttttttcatt tctcaccacc accacaatgc
     2881 aatcaggcaa agtacgtgcc cagaaatccg acggtatcgt cgagagcaca gtcaatgacc
     2941 ctaatgaatt cggctgatga ctcgaacctg gctaagaaga atttctcaga gatgaaaatt
     3001 tgtggctgct ttttctatca agaacgcgaa gaaacgtgaa gcgcaacaca aaaaacgtgg
     3061 tcgattggag catttaacgg ctccccagtt gcttaggtat tcgacacaga gaaatctttc
     3121 aaactccgct agcagaaaca ccgttgagtt aatgtccttt ggtgcactaa gccgcgaaag
     3181 tctctgtcag gcgggcgata agcgccccaa cagtgctata gacttgcggc gccaataaac
     3241 taggccgata agtgaccaag caaaaatttc cggtggggct atcgagatac ttcaaacaag
     3301 gactctgtgc atggaaaaaa tattgagcct catcgatcac gatcaaatgc ggacacccac
     3361 tacttgctcg tccctcctcc caaagcggct agtaattcat cgacgtaatt tcgttgttgt
     3421 tgcggcgcta gcgacgagag cagcagtatg atactcagcg ctgcgttgct aaggagcctt
     3481 gttgccgccg cgggagaggg caaggcaatt tcatcgccga aggttattac cctggtacgc
     3541 tcccccatct gagcgtaatc gccttcagga tcgatgacgc aaatgcggta gcctcgctcg
     3601 acgagctgct cggcaaggag ccccgcgatc caggattttc ccgagcgcgg atcaccactg
     3661 atgacaacgt tggtaccgga agtgagaagc gtaacccggt gccctcccga gttttttccg
     3721 agcacgacgg ctcgacggcg aggagtcggc acgaccccac cgtgctccaa taattttcgg
     3781 atataaacag cgacgccggc tccgcccgcc tgcgacagcc gaccatcaac gagtgccatt
     3841 actgcgggta cggaaccgcg cgcggccact ccggtttccg cattgactag catgggtata
     3901 tcgttttccg catcgccgaa agcgatcagg tttcgttccg atcgtcccaa ctcatccagc
     3961 gcccgacgaa tcccaaccgc cttgttgatt ccgctaggga gaaccatcaa agccccgcga
     4021 ttgaaaacca actggccgtc gatgccgcat tgacggatga gctcaagaac ctcactggta
     4081 tgctgttccc atgttccgac gattactgct cctgtatgaa acggcacacc gtgggctcgt
     4141 agctcgccga gaaaatgctc cgttggcggt tcgccgagct ggatcgttcg cctgagagtg
     4201 caaagataaa ttagcgcgcc gttttcggcg acgacggcat cgaaaggcga taactcgccg
     4261 caggcgtttt ggacatcctc caagactcgt cccgtgacta ataacgtgac gattccttgg
     4321 gcccgcgccg ccgatagtgc ggcatacaac tcgggtgcag gatgtccgtc atcggctccg
     4381 gtaccgtcga agtcgcaggc aataacgcgg caatacatcg ggactttgcc tcaagccgct
     4441 ctcttgttgt gtccgtttac caaggcgacg attcgtggat tgaccagagc tgtgaagtcc
     4501 ttgtatctca atcttactgt ctgcacgtga ttaccggcgt tgaagtagat gtattcatct
     4561 tttgccaacg ccggatcgac gaacaccttg agcccgaaaa ggtttccgaa cggcggcatc
     4621 gctccgatct cacattccgg aaaacgggag ataaattcat cctccgttgc catgcgcact
     4681 cggcccgcac ccagacttgc tctcaccgtc tctgggttga tcttgtgatt gccggcaatc
     4741 acgcccataa caagctcgtc atcgacttcg agcatcacca cctttgccat ttcattgccg
     4801 gaaaaatgtt gcttcgccgc gatctcctgc gccgtataag cgagagcatg gttgtaaacc
     4861 tcataaggaa tcttcgagtt gtcgagaact tctttgagtc ttttcaaaat cggcataatt
     4921 tcctcctcca ttttccgcgt tcaccttggt ttttaagaaa ggtcttgctc atggaactgc
     4981 gcaagaaata tgcccgccgt
//
LOCUS       DASULZ010000652         2232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1494627, whole genome shotgun sequence.
ACCESSION   DASULZ010000652 DASULZ010000000
VERSION     DASULZ010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494627"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..651)
                     /locus_tag="VFY96_13570"
     CDS             complement(163..651)
                     /locus_tag="VFY96_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607546.1"
                     /translation="MSTDIAAQRIGAGAAAGLIGTALIQGLMAASQKWAPESLPPIKQ
                     DPGEFMIAKAEEQLPATLREKIPEQFEKAAAKSLAFGYGTTFGALYATSRPETKSLLL
                     EGSALGLAAWGVGYLGWLPASGLMPPVAKQEPEQIAGPILSHILFGIVTVGLYRLFRR
                     LS"
     gene            843..1952
                     /locus_tag="VFY96_13575"
     CDS             843..1952
                     /locus_tag="VFY96_13575"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX5607547.1"
                     /translation="MSDIFISYARADKDKAELLANAFSRKGWSVWWDRHIPPGQSFDE
                     TIESALNSARCVIVLWSKHSVSSRWVKTEAAEGAERGILVPAMIDNSPIPLEFKRIEA
                     ADLSDWEGAASHREFDQLLKSVAGMLDGTAAPSMRRMALHGEVRSRLGWKRLGVFALT
                     GGLAGIALIFLLRQGFFTNENQSPHAQNQEQPASSDEATRLARPVGPDPAGSEAKPAR
                     SAQASAAVMNLIAAENGGHVIVATNDRWPYTIDGDEKNWQYIDIGVTGSWAVYGFKDD
                     KPARFDTFKVLILGSESWNLKEFELLAGNDSPTGKFESIGKFQTQNVRFFKDPYQEFK
                     FPPVKARYLKVRVISSHGFSSVGAYEFQLLGLLES"
     gene            complement(1978..>2232)
                     /locus_tag="VFY96_13580"
     CDS             complement(1978..>2232)
                     /locus_tag="VFY96_13580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5607548.1"
                     /translation="DGTSALQLLNEKNYDLMICDVVMPRLNAFDILEHIKSRSLSTLV
                     ILITGHPYLLADKGLGHLTSFTKPFNMYDLLRKVNEMLGK"
BASE COUNT          501 a          603 c          606 g          522 t
ORIGIN      
        1 aaacttgtcg gctagcagga gctcccaatc cgacacctaa cacggcccag gtgccggcga
       61 tcatctctat ttctcgcagc ggcagattgg cgtgtttcac tctcacacct cacatcagtt
      121 tagatacgac gatgaagctg aagcaggcaa atgctacggg ccttaggaca gtcgccgaaa
      181 gaggcgatag agtccgacgg tcacgattcc aaataggata tgactgagga taggcccggc
      241 gatttgctcg ggctcttgct tggccaccgg cggcatgagg ccgctcgctg gcagccagcc
      301 gagatagccc acaccccacg ctgccaaacc caaggcgctg ccttcgagta ataggctctt
      361 ggtctccgga cgggacgtgg cgtacaacgc gccgaaggtc gtcccgtaac caaacgctag
      421 cgatttagct gcggctttct cgaattgctc gggaattttc tcgcgcagtg tggcgggtag
      481 ctgctcctcg gcttttgcga tcatgaactc gcccggatcc tgtttgatcg gcggcaagct
      541 ttctggtgcc catttctggc tggctgccat taatccctgg ataagtgcgg ttccaatcaa
      601 tcctgcggcc gctccggcgc cgattctttg cgctgcaata tcagtactca taattgttct
      661 ccttcattgc aaaacagagg acagcaatcg gcatgccgaa ctaccctcgg tcgaaagaac
      721 cactgacgct gttttctagc gaacaacccc tcaataccct ctgtgcacct ttttttggat
      781 tagacatata gtgtcttcca gctcggattg aattctccac cgcatggaat catcatagcc
      841 gcatgagtga catttttatc agctatgcgc gagccgacaa agataaagct gagctgctcg
      901 ccaatgcatt ttcccgcaag ggttggtctg tgtggtggga ccgacacatt ccgccggggc
      961 agtctttcga cgaaacgatc gaaagtgcct tgaactcggc ccgctgtgtc attgttctct
     1021 ggtcaaagca ttcggtgtcc tcgcgctggg tgaaaacgga agcggctgag ggtgcggagc
     1081 gtggcattct cgtccccgca atgatcgaca attcgccgat tcccctcgag ttcaaacgca
     1141 tcgaagcggc ggatctctcc gattgggaag gagcggcttc ccatcgtgag ttcgatcagc
     1201 tgctaaagag cgttgccggg atgctcgatg gcaccgcagc gccatccatg aggcggatgg
     1261 cgctccatgg cgaagtacgc tcacgcctcg ggtggaagcg tctcggcgtt tttgcgctca
     1321 ccggaggttt ggccggtatc gctttgattt ttttgctccg gcaaggattt ttcacgaatg
     1381 aaaaccaatc gccccacgct cagaaccagg aacagccggc ttcatccgat gaagcaactc
     1441 ggctagcgcg gccggttggg ccagacccgg ccggttccga agccaagccg gcgcgttcgg
     1501 cacaagcttc agcagcggtg atgaatctta tcgctgccga aaacggcggt cacgtgatcg
     1561 ttgcgaccaa tgatcgttgg ccctatacga tcgacggcga cgaaaaaaat tggcagtaca
     1621 tcgacatcgg tgtgactggc agctgggcgg tttacggttt caaagacgac aaaccggcaa
     1681 ggtttgatac cttcaaggta ctaatcctcg gttcggagag ctggaacttg aaggaattcg
     1741 aactgctggc gggaaatgat tctcctaccg gtaagttcga gtccatcggc aagttccaga
     1801 cacagaacgt ccggttcttc aaagatccat accaagagtt caagtttcca ccggtcaagg
     1861 cgcgctatct aaaagtaagg gttatctctt cgcacggctt tagttccgtt ggcgcgtatg
     1921 aattccaact gctcggtttg ctcgaaagtt gatggcctac aagcaggtta caggctatca
     1981 ctttcctaac atctcgttca ccttacgtag taggtcgtac atgttgaatg gcttggtaaa
     2041 cgaagttaaa tggccgagcc ctttatcggc gagtaaataa gggtgtccgg tgatgaggat
     2101 aaccaaagtc gataacgagc gggactttat atgctcgaga atgtcgaacg cattgagacg
     2161 cggcatcacg acatcgcaaa tcatcaggtc ataatttttt tcgttcagga gttgaagcgc
     2221 gctcgtgccg tc
//
LOCUS       DASULZ010000653         2745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_75117, whole genome shotgun sequence.
ACCESSION   DASULZ010000653 DASULZ010000000
VERSION     DASULZ010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75117"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VFY96_13585"
     CDS             <1..528
                     /locus_tag="VFY96_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX5607549.1"
                     /translation="EALQALPEQIDAVLELNDQIKEIAARYANVNGFLFFGRQFNFPI
                     ALEGALKMKEITYLFAEGHPSAELKHGVIALIRPDLPSIFIAPDDSVFSKNLNNIEQV
                     KARKGPIIALTSGNCTKHLKGIADEIVLLPEAPDYVMPILSVIPLQLLAYHVAVELGR
                     DVDKPRNLAKSVTVE"
     gene            complement(569..895)
                     /locus_tag="VFY96_13590"
     CDS             complement(569..895)
                     /locus_tag="VFY96_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607550.1"
                     /translation="MQTVIQVIATGTGSLRNKIMSDPQLEKKFGFIKVWHKQPGRPHG
                     WAKIHSARDVHGAINLEWHARSRTLICRVVTKLGNKPNSIIGDFVDYLLARHQSRILA
                     IHIMRR"
     gene            complement(978..1484)
                     /locus_tag="VFY96_13595"
     CDS             complement(978..1484)
                     /locus_tag="VFY96_13595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX5607551.1"
                     /translation="MLVASMKIRHATAADCDAIWNIFHEVVADGDTYGLDPKIAREDA
                     LAYWFAPGTHTYVAEQPDTGIAGTYILRPNQSGGGSHVANAGFMVSASARGQGIGRAM
                     AEHCLNEARRLGFRAMQFNYVISTNTAAIRLWHHLGFDIVGTLPQAFHHPEKGYVDAY
                     VMYRSLQA"
     gene            complement(1521..2153)
                     /locus_tag="VFY96_13600"
     CDS             complement(1521..2153)
                     /locus_tag="VFY96_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607552.1"
                     /translation="MAGDGPGDVQNTVTKVQTAALGFRVKSGWAAVVLLTGTARSPQI
                     LDIGQIELCDPRLPETRQPYHAAMGKLETDSAEINQRQRVVRSISQQSLRKLLNGYQQ
                     KGFRIKRGALVVGSQIDPANIANPHIRAHALEGRLFRSVVEQSLQDHEIRTDLLLERD
                     AYSTAGERLKRSSDDLKQAIQDLGRSAPAEAGPWRAEQKLAALAAWSVLG"
     gene            complement(2114..>2745)
                     /locus_tag="VFY96_13605"
     CDS             complement(2114..>2745)
                     /locus_tag="VFY96_13605"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX5607553.1"
                     /translation="LAVLKAEIKPQKLLRFLDLNKTGELLVGAKVVVVGSALAGNEGT
                     AREATISGRSAERLELAGATAATSVGSPVVNENGDVIGIVTSAGEKTVARPVAALDTV
                     LSRVTPETQARWPQVAQASPTPRATPKPRLLYAPAPSFPPGQSLPGQSGTGRFRLSFD
                     SQGNVTNIQVVSSTGNPYFDQAATKTLRQWKSAPSQGWQVTVPVTFKTR"
BASE COUNT          581 a          794 c          735 g          635 t
ORIGIN      
        1 gaagcgctac aggcgttgcc cgaacaaatc gacgcagttt tggagctgaa cgatcagata
       61 aaggaaatcg cggccagata cgcgaatgta aacggttttc tgttcttcgg gcgacaattc
      121 aatttcccga ttgctctcga aggcgccctg aaaatgaagg agatcacgta tctctttgcc
      181 gaaggtcacc caagcgcgga acttaaacac ggcgtgattg cactgattcg tcctgatctg
      241 ccctcgattt tcatcgcgcc ggatgattcc gttttttcaa aaaacttaaa caacatcgag
      301 caagtgaaag ctcgcaaggg gccgatcatt gcgctgacaa gcggtaattg tacgaaacac
      361 ctgaaaggaa ttgccgacga gatagtcctc ctgccggaag cgcccgatta cgtgatgccg
      421 attctctccg tcatcccgct ccagcttttg gcgtatcacg tcgctgtcga actcggccgc
      481 gacgtcgaca aaccgcgcaa cctcgccaag agcgtgacgg tcgagtagtt gggcgcctaa
      541 cgaggtctcc aaacgtgcgg cttcgaattc accgtcgcat gatgtgaatc gcgagaatgc
      601 gactctgatg ccgcgccagc agataatcga caaaatcgcc aatgattgag ttcggcttat
      661 tgccgagctt tgtcactacg cggcaaatca atgttctgct gcgcgcatgc cactccaaat
      721 tgattgctcc gtgaacgtcg cgagcactgt gtattttggc ccagccatgc ggacggcccg
      781 gttgtttatg ccagactttg atgaagccga attttttctc caattgcgga tcgctcatga
      841 ttttgttccg caacgatccg gtcccagtcg cgataacctg aatcaccgtc tgcattccac
      901 cctctaggtt cagcaggaaa tcattcgttt ggcaatcctt gtttgcctga caggatacga
      961 ccgccgcgtg acgcgcatca cgcctgcaag gaccggtaca ttacgtaagc gtcgacgtat
     1021 cctttttctg gatgatggaa agcctggggc aaagtgccaa cgatgtcgaa gccgaggtgg
     1081 tgccagagcc ggatcgcggc ggtatttgta gaaatcacat aattgaattg catggcgcgg
     1141 aaaccgagcc ggcgcgcttc gttcaagcaa tgctcggcca tcgcacgacc gatcccctgc
     1201 ccccgagcgc tcgctgagac catgaatcct gcgttggcga catgcgatcc tcctcccgat
     1261 tggttaggcc gcaaaatata agtaccagcg attcctgtat cgggttgctc cgctacatac
     1321 gtgtgcgtac ccggcgcaaa ccagtacgcc agcgcgtcct cgcgcgcgat ctttggatcc
     1381 aatccatatg tatccccatc tgcgaccacc tcgtggaaaa tattccagat tgcatcgcaa
     1441 tccgctgcgg ttgcgtggcg aattttcatg cttgcgacca acattgtaag acacccgctt
     1501 cgattgccgc aattccgcgg ctatccgagc acagaccacg cagccagcgc ggcgagcttt
     1561 tgttctgcgc gccagggtcc agcctctgcc ggcgccgatc gtccgagatc ttgtatcgcc
     1621 tgtttcagat cgtcgctgga gcgtttcaag cgctcacccg ccgtagagta ggcatcgcgt
     1681 tcgagcagaa ggtcagtgcg aatctcatgg tcctgcaagc tttgctctac taccgagcga
     1741 aataagcggc cttcgagggc atgcgctcga atgtgcggat ttgcaatgtt agccgggtcg
     1801 atctggcttc ccaccacaag cgcgccacgt ttaatccgaa atcctttttg ctgatagccg
     1861 ttaagaagtt ttctaagtga ctgttgcgaa atgcttcgca ccacgcgctg gcgctggttt
     1921 atttctgctg agtctgtttc gagttttccc atcgccgcat gatacggctg tcgtgtttcg
     1981 ggaagccggg gatcgcacag ctcgatctga ccgatgtcta agatctgcgg ggaacgcgcc
     2041 gttcctgtca ggagtacaac cgcagcccaa cctgatttca cacgaaatcc aagcgcggca
     2101 gtttgaactt tggtcaccgt gttttgaacg tcaccgggac cgtcacctgc catccctgac
     2161 tgggagcgga cttccattgt cgaagcgttt ttgttgcggc ctggtcaaag tacggattcc
     2221 ccgtggacga gacgacctgg atgtttgtga cgttcccttg tgaatcgaag ctcaatcgga
     2281 aacgacccgt cccggattgt ccgggcagcg actgccccgg aggaaacgat ggcgcaggtg
     2341 catagagcaa tcgcggcttc ggcgttgccc gcggcgtcgg gcttgcttgg gcaacttgtg
     2401 gccagcgcgc ctgagtctca ggcgtaacgc gggacaagac tgtgtctaag gcagcgacag
     2461 ggcgggctac agtcttttct ccggcagatg taacgatacc aatcacgtcg ccattctcat
     2521 ttacgaccgg cgacccgacc gaagtcgctg cagtagcgcc cgctaattcc aatcgctcgg
     2581 ccgatcgtcc gctgatggtc gcttcacggg ctgtgccctc gttcccagcc agcgcgctgc
     2641 cgaccactac gacctttgcg ccgaccaaaa gctcgccagt tttgttcagg tcgaggaatc
     2701 tcaaaagctt ttggggcttt atctcggcct tgagcaccgc caaat
//
LOCUS       DASULZ010000654         2711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1087650, whole genome shotgun sequence.
ACCESSION   DASULZ010000654 DASULZ010000000
VERSION     DASULZ010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087650"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1395
                     /locus_tag="VFY96_13610"
     CDS             151..1395
                     /locus_tag="VFY96_13610"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX5607554.1"
                     /translation="MKRECVVSLFFVFVLYVGSALVWADPPSQFPQNATFTTLITTPR
                     AIEGLTGDNHRNLYTGGSGAPPCPVWQINIHNPKLIEVGFVVPASGNCGFSGIAFDEA
                     ENLYVADGAAGIIYFFNPNTKNPPTATIFAQGVPGTNGLAFDRRGNLWTGDGTTGQGR
                     VWKITSPGANCTPPTPVNCVEVFRIQPMANELDPATTGGVSNVGRDVRTLPPGTITVT
                     PTSRNASNMLGSQALVANGLAFNKNGDLFNIDTARGALWKIEFDGRGELRSPTGCDTT
                     FTRNTLCLSNVFVAHPILEGGDGIALDLEGNIWVDANERNAVALVTKDRRVFEVFRNP
                     VNSAGSRNSADTAEGNTHILEFPNSPFLMGRLFCTSQSDGNRRDNFPNTAGQVSPTGP
                     NIGKISCMDQELTIPGLHLPVR"
     gene            complement(1662..>2711)
                     /locus_tag="VFY96_13615"
     CDS             complement(1662..>2711)
                     /locus_tag="VFY96_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607555.1"
                     /translation="MCLLSTALKADAAEKPPFYQGKTLNVVINFGAGGPTDIESRIFA
                     RHLSRHIPGAPTITVQNMGGGGGLTAVNYIGEVAKPDGLTAAYFTGSLFQHQVKDPAL
                     RVDIGKFGFITGVHGVTVSYIRADVPPGIKKPSDFVKAQKFRAAGLGVSSSKDVRFRL
                     SFDLLGLKYDYVTGYNNSSDARLAVQRSEAQYHDETLPSYRSQVEPQMVKTGMVTPIY
                     YTDLVAPSGEILVSRDVPELQPFTYYYKEIFGKLPSGIKYEALKAANMSSTNMTRMVL
                     MPPKVPAECIAILRKAFDELSRDQDFLQEAISAMRFQPRFEVGEAGEKLFKQASAVSP
                     EVVNFLRKYIEEANR"
BASE COUNT          624 a          715 c          731 g          641 t
ORIGIN      
        1 aaagcgtctt tgctctaatc gctcaaagtc ccttttgact cctgctggat ttcccatgcg
       61 gagctattta ccatacgtgc cgtcctaagt cactatattt tatgaacctc catagtagag
      121 acgcgaaagc acaatagaga agaggcaact atgaaaagag aatgcgtcgt gtcgttgttt
      181 ttcgtgtttg tactttacgt tggttcggct cttgtctggg ccgatccgcc gtcccaattc
      241 ccacagaatg caacatttac aacgctcatc acgacaccgc gagcgataga ggggttaacc
      301 ggagataatc accgcaatct atatacgggt ggttcgggcg cgcctccctg ccccgtttgg
      361 caaatcaata tccacaaccc caaactgatt gaagtcggat tcgttgtgcc tgccagcggg
      421 aactgtggtt tttccgggat tgcgtttgat gaggccgaga atctgtatgt tgctgatggc
      481 gctgccggaa tcatttattt ctttaatcct aatacaaaga acccgcccac tgcgactatc
      541 ttcgcgcagg gcgtcccagg gacgaacgga ttggcgttcg atcggcgcgg caacctctgg
      601 acgggcgatg gaaccacggg ccaaggaaga gtttggaaga taaccagccc cggggccaac
      661 tgtacccccc cgactccagt caattgtgtt gaggtttttc gcatccagcc aatggcaaat
      721 gagcttgatc cagcaacgac cggcggtgtt agcaatgtgg ggcgcgacgt ccgaacgctg
      781 ccgccaggga cgataaccgt gacaccaacg agccgcaatg cctccaacat gttgggttcc
      841 caggcgctcg tagcgaacgg tttagccttc aacaagaatg gcgacctttt caacatagat
      901 actgctcgcg gagcgctttg gaagatcgaa ttcgacgggc gcggggaact taggagccca
      961 actggctgcg ataccacctt tacgcgtaac acgctctgcc tgagcaatgt gttcgtcgct
     1021 catcccattc tcgagggagg cgacggcatt gccctcgatc tcgaagggaa catctgggtg
     1081 gacgcgaacg agcgaaatgc tgtggcactg gttacgaaag atagaagagt gtttgaagtg
     1141 ttccgcaatc cggtgaactc ggccggttcg agaaattctg cggatacagc agagggaaac
     1201 acgcacattc ttgaatttcc caacagtcct ttccttatgg gaagattgtt ctgcacatcg
     1261 caatctgatg gcaaccgacg cgacaatttc ccgaatacag ccggccaagt cagtcccacg
     1321 ggacccaata tcggcaaaat ttcctgcatg gatcaggaac tgacaattcc aggattacat
     1381 ctgccggttc gctgactctt ggttttgaca tacgatctct gcgatgagaa gcctctggcg
     1441 tcaccagagg ttttcttatt tgaagccggc ggacctgcca ctgaacaacc gagaaatttc
     1501 gaattcggga tcaacttaaa atacgcgaag cagatcggtc tgacggttcc caatagcgtg
     1561 ctatatcggg cagacaaggt tattaaataa tttttttgtg agcggcgcag gtggttgctt
     1621 aggaaatccg gctttcgacg atttctctca acgcattagt gttatcgatt cgcctcttcg
     1681 atgtacttgc gcaaaaagtt caccacttcc ggcgaaacgg ccgaagcctg cttgaataat
     1741 ttttcgccgg cttcgccgac ctcgaagcgc ggctgaaagc gcatcgctga gatcgcttcc
     1801 tggagaaagt cttgatcccg tgacagttcg tcgaaggctt tgcgcaaaat cgcaatgcat
     1861 tcggccggga ctttcggcgg cattaaaacc atccgtgtca tattcgtgct cgacatgttg
     1921 gccgctttca gtgcttcata tttgatcccc gatggtagct tgccgaaaat ttccttgtaa
     1981 tagtaagtga agggttgaag ctcgggaacg tcgcggctta ccagaatttc gccactcggt
     2041 gccactagat ccgtgtaata gatgggcgtc accatgccag tcttgaccat ctgcggctcg
     2101 acttgggagc ggtagcttgg cagagtttca tcgtggtatt gggcctcgct gcgctgcacc
     2161 gcgagccgcg catcggagct gttgttgtag ccggtcacgt aatcgtactt gagcccgagc
     2221 agatcgaaag agaggcggaa acgcacgtcc ttcgaactgc tgacccccag ccctgcggcg
     2281 cgaaatttct gcgccttgac gaaatccgaa ggtttcttga ttcccggtgg cacatcggcg
     2341 cggatgtaag aaacggtaac gccatggact ccggtgataa agccgaattt accgatgtct
     2401 acccgtagcg ccgggtcttt aacctggtgc tgaaaaaggc ttccggtaaa gtaggccgcg
     2461 gtcaagccgt ccggcttggc gacttcaccg atgtaattga ccgcggtcag gccgccaccg
     2521 ccgcccatat tttgcacggt aatggtcggg gcaccgggaa tgtgccgtga caagtgcctg
     2581 gcgaaaatac gggattcgat gtcggtcggg ccaccggcgc caaagttaat cacaacgtta
     2641 agggtcttgc cttgatagaa cggcggtttt tccgcagcgt cggctttcag cgcagtgctc
     2701 agaagacaca t
//
LOCUS       DASULZ010000655         2399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_277014, whole genome shotgun sequence.
ACCESSION   DASULZ010000655 DASULZ010000000
VERSION     DASULZ010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277014"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VFY96_13620"
     CDS             <1..470
                     /locus_tag="VFY96_13620"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HEX5607556.1"
                     /translation="TFHGNAQILSEAKLAGGGLRLQLQYVDVREPKDIEIAFREAKKG
                     RAEAVLVLAGPVFFFERRQLAGLAVKSRLPAIYYATEFVEDGGLITYGANRAELHRRA
                     SVYVDKILKGAKPADLPIEQPTKFELVINLRTAKQIGLTIPPNVLARADRVIK"
     gene            566..1465
                     /locus_tag="VFY96_13625"
     CDS             566..1465
                     /locus_tag="VFY96_13625"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607557.1"
                     /translation="MPRIAYLTVAPLSANVARVEAFGQGLHELGYVDGKNIVIEWLSG
                     EGKLNTESELVAEAVRLKVDVIVTSGPTMTRAAKQATTTIPIVMTFDSDPIDSGFVVS
                     LARPGGNITGLSALSPELSGKQLELLKEIIPKLSRVAILGDSKEPANPKILKEIELSA
                     ASFGIRVQPLDVLTRRDIETAFHSATEARSHALVVLASLVLSNHRARVAHLALKNRLP
                     AIYFRQEFVEAGGLMSYGTSFTDLSRRAATYVDKILKGAKPAELPVEQPTKFELVINL
                     KTAKQIGLIIPPNVLARADKVIR"
     gene            1462..>2399
                     /locus_tag="VFY96_13630"
     CDS             1462..>2399
                     /locus_tag="VFY96_13630"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607558.1"
                     /translation="MKLLNNRCPAASSTFALCALLFVLCVSADAQQAIKIPRIGYISG
                     TGNATDQGPYVESLRRGLRDLGHVNGRNIVIEFRGAEGKLDRVKGLISELVHLNVDIL
                     VIPLRGGILAARDATKTTPIVMVAGIDPVADGLVNNLARPGGNITGLSTLSRELGGKR
                     FELLTEMVPQLYRVGVLKNSDEPSSAAGIFEQYQTAAKALNIEIRSLEVGGPNPDLEG
                     AFRTAIKERLRAIITITSAPLFLQRKRIVDLALTNRIATAYQGSVWVEAGGLMSYSSD
                     DIEAFRRAAWYVDKILNGTKPAELPIEQPTKFELVI"
BASE COUNT          607 a          614 c          654 g          524 t
ORIGIN      
        1 cgacctttca cggtaacgca caaatcctca gtgaggcgaa actcgccgga ggggggctca
       61 ggttgcagct tcaatacgta gacgtgcgag agcccaaaga tattgagatt gcatttcgag
      121 aggcaaagaa ggggcgtgct gaggcggttc tcgtcctggc gggtcccgtc ttcttttttg
      181 agcgaagaca gctcgcaggc cttgcggtaa agagccggct tccggcgata tactacgcga
      241 cagaatttgt cgaagatggc ggcctgataa cctatggggc gaatcgcgcc gaattgcacc
      301 ggcgagcttc cgtttacgtc gataagattt tgaaaggggc aaaacctgcc gacttgccga
      361 tcgaacagcc gacaaaattt gagcttgtaa tcaatcttag aaccgcaaag caaatcggcc
      421 tgacgattcc accaaatgtg ttagcgagag cggatagagt gatcaagtga cactccccgg
      481 tggtaagcac tcagtagtca gcctattgct atacgccatg ctctttgcac ttggctcgtc
      541 cagcgacgcg cagcagccaa ataaagtgcc gcggatagcc tatctgaccg ttgcgccgct
      601 ttccgctaat gtcgcgcgcg tcgaagcgtt tgggcagggc ctgcatgagc tcggttatgt
      661 ggacgggaaa aatattgtca tcgagtggct gtctggcgaa ggaaaactca acaccgagag
      721 cgaactggta gccgaggcag tgcgtctcaa agtggacgtc attgtgacga gtggtccgac
      781 gatgacccgg gcggccaagc aagccacaac taccattccc atcgtcatga cgttcgatag
      841 cgatccaatt gacagcgggt tcgtcgttag cttggcgcgg cctggtggaa atatcacagg
      901 actgtcagcg ctatctccgg agctgagcgg caaacaattg gagctgctga aggagatcat
      961 tcccaaactt tcacgcgtag caatcctcgg agactcgaag gaacctgcca atcccaaaat
     1021 cttgaaggag atcgaattaa gcgcggcgtc atttggcatt cgagtacagc ccctggatgt
     1081 cttaacccgc agggatattg agactgcatt ccacagcgcg acagaagccc gcagtcatgc
     1141 gcttgtggta ctcgcttcgc tagttcttag taatcaccgg gcaagggttg cccatctagc
     1201 gctcaaaaat cggctgcccg caatatattt taggcaggaa tttgtcgagg ccggcgggct
     1261 tatgagttat ggaacgagct ttacggactt gtcgcgacga gctgccactt acgtggacaa
     1321 gattctcaaa ggcgcaaagc ctgctgaact ccccgttgaa cagccgacaa aattcgagct
     1381 agtcatcaat ctgaaaacag ccaagcaaat tggcctcatc attccgccga acgtgctggc
     1441 gagagcggat aaggtgatca gatgaaacta ctcaataatc ggtgtccagc agccagctca
     1501 acttttgccc tgtgtgcttt gctctttgtg ctttgcgtct ccgccgacgc acagcaggcc
     1561 ataaagatac cgcgcatcgg ttacatatca gggactggca acgctacaga ccagggccct
     1621 tacgtcgagt cactacggcg cgggctgcga gaccttggcc acgttaatgg aagaaacatc
     1681 gtgatcgagt ttcgcggggc cgagggaaag ctggaccgtg taaagggcct cataagtgaa
     1741 cttgtacacc tcaatgttga tattcttgta atcccacttc gagggggcat tcttgccgca
     1801 agagacgcga caaaaacaac tccaattgtc atggtagcgg gtatagatcc agtagccgat
     1861 ggcctcgtaa ataatcttgc gcgaccgggt ggcaacatca cgggcctgag tacgcttagc
     1921 cgagagttag gcggaaagcg gtttgaattg ctcacagaaa tggttcctca attgtatcgc
     1981 gtcggagtac tcaaaaactc agatgaacca tcctccgctg ctggtatttt cgagcagtac
     2041 cagactgcag cgaaggcttt gaacatagaa atccgatcct tagaagtcgg cggtccaaat
     2101 cctgatctgg aaggagcttt tcggactgcc atcaaagaac gtctgagggc aattattacc
     2161 atcacgagcg cgccgctttt tctacagcgg aaaagaattg tcgatttagc actgacgaac
     2221 cgtatagcta ccgcatatca aggaagcgtc tgggtggaag cgggcgggct catgtcttat
     2281 tccagcgacg acattgaagc ctttcgccgc gctgcttggt atgtagacaa aatcctgaac
     2341 ggaacaaagc ccgctgagct tcccattgaa caaccaacca agttcgagct ggtgatcaa
//
LOCUS       DASULZ010000656         2652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1190868, whole genome shotgun sequence.
ACCESSION   DASULZ010000656 DASULZ010000000
VERSION     DASULZ010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190868"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..1193)
                     /locus_tag="VFY96_13635"
     CDS             complement(228..1193)
                     /locus_tag="VFY96_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5607559.1"
                     /translation="MESSDLRVFEAVARLGSITAASDELHTVQSNVTARIHALEDQLG
                     VALFRRHSRGVLLTSAGERLLPYAIKVAQLLQDATHVVGDQTMPAGRLLIGSLESTAG
                     VRLPPILSAYRERFPQVNLVLTVGTTGHLIEEVLARRLEGAFVAGPVDRAEIVEVQVF
                     AEEMVLVTSPKFRSLDEVFQSPLGVNILVLREGCSYRARLERLLKENGVGSFGQLEFG
                     TIDGIFGCAREGLGITMMPRSVSERASQNGKLGIHVLPQKEAMVPTMFIRRKDAVESA
                     ALRRFLECALACASAAAAQTPPNRKLKKHPKLSTVPFASQANRRE"
     gene            1366..2451
                     /locus_tag="VFY96_13640"
     CDS             1366..2451
                     /locus_tag="VFY96_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF561 domain-containing protein"
                     /protein_id="HEX5607560.1"
                     /translation="MSIQTSLTTRLGMTWPIIQAPLAGGGDTPALVAAVCNAGGLGFI
                     GAAYLSPEQIGNAAREVRARTTKPFGISLFAPLPAPDLPVDPKPALERVAPFFSELGL
                     PPPVVAASPHLSFDEQLAAALETDAAVFSFTFGILPRQAIESVKTRGMFLIGTATTVD
                     EAVALEKAQVDAIVAQGSEAGGHRATFGSDFDSGMIGTISLVPQVADAVAVPVIASGG
                     IMDGRGIAAALALGASAVQMGTAFLTCEEAGIPEVYKRAILQAREHETRLTRAFSGRP
                     ARGIVNRFMVEFEPSDASGDILPFPLQNDLTRPLRSAAAKLGRAEFLSLWAGQGIRLA
                     QRRMAAQLVECLAVETETAIRRLAQTK"
BASE COUNT          516 a          800 c          793 g          543 t
ORIGIN      
        1 gggaaattaa cgggcagacc tttactcccg actctctttc cttttttttc atcccgcaga
       61 ggcagttttg aattcagctc cagacttctt gcttgcgaga gcgtgcgccg ccgcggaccg
      121 cgccttgcgc catgcaatct gcggatgatg aggccggctc ggccgcaatc cgtgccggcg
      181 ataacctcgg atgcacgagg aaatctatcg aaccggcggg gctcatttta ctcgcggcgg
      241 tttgcctgac ttgcaaatgg aacggtcgaa agtttcgggt gctttttgag ctttcgattc
      301 ggcggagttt gcgctgccgc ggcgctcgcg catgccagcg cgcactcgag aaatcgacgc
      361 aacgcggcgg attctaccgc gtcctttcgg cgaataaaca tggtcgggac catggcttct
      421 ttctgtggca gcacgtgaat tcccaacttg ccgttttgcg aggcgcgctc cgacaccgaa
      481 cgcggcatca tcgtgatacc gagaccttct cgagcgcagc cgaagatgcc gtcgatggtg
      541 ccgaattcta attggccgaa agaaccgacg ccattctcct tcaacagccg ttcgaggcgg
      601 gcacgatagg aacagccctc gcgcaagacg aggatgttca cacccagcgg cgattggaaa
      661 acttcatcaa gtgagcgaaa tttcggtgac gtgacgagaa ccatctcttc ggcgaaaact
      721 tggacctcca cgatttcggc acggtcgacc gggcccgcga cgaatgctcc ttccagccgc
      781 cgcgcgagca cttcttcaat caaatgaccc gtggtgccga cggtcaggac gagatttacc
      841 tgaggaaatc tctcccggta agcggatagg atcggtggca accgcacgcc agcggtggat
      901 tcgagggagc cgatcagcag acggccggcg ggcatcgttt ggtcgcccac gacatgggtc
      961 gcgtcctgca aaagttgcgc gaccttaatc gcataaggca gcagccgctc gcccgcggat
     1021 gtgagaagga caccgcggct atggcggcgg aacagggcaa cacccagctg gtcttccagc
     1081 gcatggattc gtgccgtgac attcgattga acggtgtgta gttcatcgga tgccgccgtg
     1141 atgctcccca agcgggcgac ggcttcgaac actctgagat cgctgctctc catttcactt
     1201 tccctacgaa aaactatagc acggtcggac ccggcgttcg aaatcactgg ttcagatagc
     1261 agtcatcaaa acaaagaatt ttgttcggcg gctgaagcgc gttagcgcta accgtacgga
     1321 cgcgagaaca tcaaacctcg gagatcttga tccgttgagg tggctatgtc gatacagaca
     1381 agtttaacga cgcgcttggg aatgacctgg ccgattatcc aggcgccgct cgccggcggc
     1441 ggtgatacac cggcgctcgt cgccgccgtt tgcaatgccg gagggctcgg cttcatcggt
     1501 gcggcctacc tgtcacccga gcagattggc aacgcggcgc gcgaagttcg ggcgcggaca
     1561 acaaagcctt tcggcattag cctgtttgcg ccgttgcctg cgccggatct acccgtcgac
     1621 ccaaagccgg cgctggagcg cgttgcgcct ttcttttccg aactgggact accgcctccc
     1681 gttgtcgccg cctcgccgca tctgtcgttc gacgaacagt tagcagccgc tctcgaaacc
     1741 gacgctgcgg tattcagttt caccttcggc attctgccaa ggcaggcgat tgaatctgtc
     1801 aagacccgcg gcatgtttct gatcggcacg gccacaactg tggacgaggc ggtcgcgctc
     1861 gaaaaggcac aagtcgatgc gattgtggcg caggggagcg aagcgggtgg acaccgcgcg
     1921 accttcggat ctgattttga ctcgggcatg atcggaacca tctcgctcgt gcctcaagtc
     1981 gccgacgcgg tcgcagtgcc ggtgatcgcg tcgggcggga ttatggacgg ccgtggcatc
     2041 gctgctgccc tggcgcttgg cgcaagcgcg gtgcagatgg gcactgcgtt cctgacttgc
     2101 gaggaagcgg ggattccgga ggtttacaaa agggcgatcc tccaagcgcg cgagcatgag
     2161 acccggctaa cccgcgcgtt ctccggtcgt cccgcccgcg gcatcgtcaa ccgctttatg
     2221 gtggaattcg agccgagcga cgcttccggc gacattcttc cattcccact tcagaacgac
     2281 ctgacccggc cgctccgcag cgctgcggca aagctcgggc gcgcggaatt tctctccctc
     2341 tgggcaggcc aaggtattcg cctggcgcag cggcgcatgg ctgctcaact tgtcgaatgt
     2401 ctcgcggtcg aaaccgaaac tgcaatccgt cgtttggcgc agacgaagta gctctctcgc
     2461 ggcgcattga agttgcaact tccgtggatc gaatcaccgg cgagttctcg ccaacgatgg
     2521 cgctgcaggc gtgggtcgcc cccggatcga tgcgtacagc ggccatgact tcgaactgtg
     2581 cttgtgccta atgagcgcaa ttgcatctgt attcggctcg gggattccaa gtatcctgtc
     2641 tccgcacaaa ca
//
LOCUS       DASULZ010000657         2451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_75481, whole genome shotgun sequence.
ACCESSION   DASULZ010000657 DASULZ010000000
VERSION     DASULZ010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75481"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1620)
                     /locus_tag="VFY96_13645"
     CDS             complement(<1..1620)
                     /locus_tag="VFY96_13645"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX5607561.1"
                     /translation="MLTLLEHGVSPPYSERPDTGLEIRGIDGSSLFRYGQSEFLFGKI
                     DAIPPLIVKTLLFLENRDLDRPATRWQNPVIEWDRIFKAAMMYVGAKLHLPVPVQGGS
                     TLAVQLEKFRHSPNGRTDSPLEKLRQIMGASLKAYREGANTRAWRERIIVDYLNSVPL
                     AAAPGYGEIHGLGEGLYAWFGMRLDDVSAALNTPGLTPAKIRAFKHVLTLLISVRAPS
                     VFLVDERSSLEEKVGQFTRLMARSGLIDWEMAAELQETPIRFLPTAPLPPQPSSSRNK
                     AANAIRTNMMDALGVNNLYDLNRLHLQVDSTFDADLQKNVTAFLNRLADRQFVSAEGL
                     NGHWLLENADPAKVIYSFLLVEATSSGNLIRVQADNFSAPFDFNKSVKLELGSTAKLR
                     TLTHYLEIMAALHGELAPLNEKQLQQRLQTARDPLTRWTAETLKNEKPGDLKAFLNLA
                     MERRYSGSPYEEFFTGGGVHHFENFEKEENSKIYSVREAFYHSVNLSFIRLMRDLVSY
                     HRVRLPYDSDDVLANPANAQRGKMLQEIAEEE"
     gene            2225..>2451
                     /locus_tag="VFY96_13650"
     CDS             2225..>2451
                     /locus_tag="VFY96_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607562.1"
                     /translation="MLATRVLQKEGTFYFIAYPAAELLDRVNFSSRYYFEGEHIEADE
                     PGDDEVARFIAGIEKSERSFQRLLNRRKIRQ"
BASE COUNT          589 a          630 c          678 g          554 t
ORIGIN      
        1 ttcctcttcg gcaatttcct gcaacatctt gccgcgttgc gcgtttgccg gattcgccaa
       61 tacgtcatcc gagtcgtacg gcagacgaac ccgatgatag ctcaccaggt cgcgcatcaa
      121 tcgaatgaat gacagattga ccgaatgata gaacgcttcg cgcaccgaat atattttgct
      181 gttctcttcc ttttcgaagt tttcgaaatg atgcactccg ccgccggtga agaactcctc
      241 gtatgggctt ccggaataac gccgctccat cgcgagattg agaaaggcct tcaaatcgcc
      301 cggtttctca tttttcagag tttccgccgt ccaacgggtg agcggatcac gggcggtttg
      361 caatctctgc tggagctgtt tttcattcaa aggtgcgagc tcgccgtgaa gcgcggccat
      421 tatttcgaga taatgcgtga gggtacgcaa tttggcggtg ctaccgagct ccaactttac
      481 acttttgttg aagtcgaagg gcgcagaaaa attatccgcc tggacacgaa tgagattgcc
      541 gctggacgtc gcctccacca acagaaacga ataaatcacc ttggccggat cggcattttc
      601 aagcagccag tggccgttta gtccttccgc gctgacgaac tgccgatcgg cgagccgatt
      661 cagaaacgcg gtgacgtttt tttgcaggtc ggcgtcaaag gtgctgtcga cctgcaagtg
      721 aagacgattg agatcgtaaa gattattcac gccaagggca tccatcatat tggtgcgaat
      781 cgcgttcgcc gctttgttcc tgctcgatga cggttgaggc ggcaacggag cggtgggaag
      841 gaagcggatc ggcgtctctt gcagctccgc cgccatttcc caatcgatca gtcccgagcg
      901 cgccatgagc cgcgtgaatt gcccgacctt ttcttccaaa gaggaacgtt catcgacgag
      961 aaacaccgac ggtgcgcgga cggaaatgag cagggtcaga acgtgtttaa aggcgcgaat
     1021 cttcgccggc gttagccccg gtgtattcag ggccgcactg acatcatcaa ggcgcatccc
     1081 gaaccaggca taaaggcctt cgccaaggcc gtgaatctcg ccgtaacctg gcgccgccgc
     1141 gagaggcacg ctgttaagat aatcgacaat gatgcgttcg cgccacgctc gcgtattggc
     1201 gccttcgcgg taggccttca ggctcgcgcc cataatttgg cgcagcttct cgagggggct
     1261 gtcggttcgg ccgttcggcg agtggcggaa tttttccagc tgcacggcaa gcgtgctgcc
     1321 gccttgcacc ggtaccggca aatgcagttt ggcgccgaca tacatcattg ccgctttgaa
     1381 aattcggtcc cactcgataa ccggattctg ccagcgcgtt gcgggccgat cgaggtctcg
     1441 gttctcgaga aaaagcaaag ttttaacgat caatggcgga atggcgtcga ttttaccgaa
     1501 gagaaattcc gactggccat agcgaaacaa cgaggaacca tcgataccgc ggatttctag
     1561 gcctgtgtca gggcgttcgc tgtaaggagg ggaaacgccg tgctccaaaa gcgtgagcat
     1621 ggtttccgat tggcgtgctt gttcagcgat ttgataccct tgggaggtta accgggtttg
     1681 aaaaccctgc agtttcgaat aaccctttcg ctgatcgaag ggtcccggcc ggggaaaaga
     1741 gatttcacgg ctcgatcctt gtgccagctt gtagaagacg cgccgattag tgctggtgaa
     1801 aaaccatgat tgaagcgcgg atgtttgaaa ttcgataaaa acacagacga aaaagagcaa
     1861 aaacaggcca aaccagaaga ggcagatctt tagccgcctt tttagtgccg gagaaaagag
     1921 tcgagacgtg aaggaggagg atctccgtaa ctcggacggg atggattttc cattcatcga
     1981 tgcgaagcat tagaatagca tatcccagat taaatactaa tttgattttg tccgctggtt
     2041 cgtccagctt gacaaacgtc tgtttctttg ccgtggcagc cgaagctcgg tagccaggaa
     2101 ggaatttccc tacaagcttg cacggaatgt cgcgaagccg caaaatggcg agcggattaa
     2161 caaattgtct tctagcaagc gagccacctc gatataacga gggtcggtcg agaaggagca
     2221 atcgatgttg gcaacacgcg tcctgcaaaa ggaaggcact ttctatttta tcgcatatcc
     2281 cgcagccgaa ctgctggacc gggtaaattt ctcgagtcgc tactacttcg aaggagagca
     2341 catcgaggcc gatgaacccg gtgatgacga ggtcgcaaga tttattgctg gcatagaaaa
     2401 gagcgaaaga tcgttccaac gtttgctcaa ccgccgaaag atccgccaaa t
//
LOCUS       DASULZ010000658         2990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_582370, whole genome shotgun sequence.
ACCESSION   DASULZ010000658 DASULZ010000000
VERSION     DASULZ010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582370"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VFY96_13655"
     CDS             <1..311
                     /locus_tag="VFY96_13655"
                     /inference="COORDINATES: protein motif:HMM:NF017742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HEX5607563.1"
                     /translation="KTEAARERLSDHLKTLVEGVEELLKATAEQSGDGISLLRDRLIE
                     SLEQGKRTLRQGRKILQASRDGAETAISYAHENPWAVFGVALGAGAALAFFLLSKFRR
                     "
     gene            371..679
                     /locus_tag="VFY96_13660"
     CDS             371..679
                     /locus_tag="VFY96_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607564.1"
                     /translation="MFVSRLAFHTLPGKTQMVEDKLQQLVKWVGQAGGQRPRIMRTHY
                     GSPGAPDLIFEQEVENPATLEEQIKSVTDQDAFRRWTEDVAPLLEQSSKRELYEIVSK
                     "
     gene            complement(826..1344)
                     /locus_tag="VFY96_13665"
     CDS             complement(826..1344)
                     /locus_tag="VFY96_13665"
                     /inference="COORDINATES: protein motif:HMM:NF017913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HEX5607565.1"
                     /translation="MTERPRWIAMRRWLSLSFNFVEDAVYVGLGLLLAGIAGALLIAE
                     IIYFVQYLLAGQLSENVVMLLDRILLIIIFVEVLYTVQVSFRQHVLRPGPFLVVGLIA
                     VTRRILVVTAELPKMARENDAIFHNAMIELGLLTALTVALVISLHFLRPDDPDSAAQE
                     SNRSRGAKPTAR"
     gene            complement(1448..1954)
                     /locus_tag="VFY96_13670"
     CDS             complement(1448..1954)
                     /locus_tag="VFY96_13670"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5607566.1"
                     /translation="MLPVQNILIAVDDHDGAKRAVEYVAAFVADRADVDIHLFHALRP
                     LPPQLMESPGAEDPSREQQIERRQAAQQESWKNRAGTQVVPVLEAAKSQIIAANIPAE
                     KVTTHILQLNDRGDLVSEIIRAAHEYRCHTVVVGYNDYPWIKEKFHAHIAEQLIAQSE
                     RLAVCVVK"
     gene            2155..2775
                     /locus_tag="VFY96_13675"
     CDS             2155..2775
                     /locus_tag="VFY96_13675"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX5607567.1"
                     /translation="MAYAEYKAKKFQLSGLEGISDKTLEMHFGLYEGYVKNTNLLMEQ
                     ISAISRKKQASGQDPAYAELTRRLGFEFGGMVLHEYYFENLASKGKGSPSGDFKKAID
                     ESFDGFENWQQAFTAVGGMRGVGWAILYQDPVTERLSNHWIALHQDGVPSGFKPILVM
                     DVWEHAFILDYKPADRGKYINAFFNNVNWDAVQRRLTNPAAVRAVA"
BASE COUNT          748 a          738 c          818 g          686 t
ORIGIN      
        1 gtaaaactga agcagcgcgc gaaagattat ccgaccacct caaaacattg gtagaaggtg
       61 tcgaagaatt actgaaggca accgccgagc agagcggcga tggaatatcg cttctacgtg
      121 atcgtctgat cgagagtttg gaacaaggca agcggacact ccggcaggga agaaagatat
      181 tgcaggcgtc acgggacggg gcggaaacgg ctatttcgta cgcccatgag aatccgtggg
      241 ctgtattcgg tgtcgccctc ggcgcgggcg cggcattagc ttttttcctg ttaagcaagt
      301 tcaggagatg agtccgactt gcatctttgt gcctgagact tagcggaaat aagtcaagga
      361 ggataagacg gtgtttgtat cacgacttgc atttcacacc ttgcctggca aaacacaaat
      421 ggtcgaagac aaattacaac agttggtgaa atgggtcggg caagccgggg gccaacggcc
      481 gagaattatg cgcactcatt atggttcgcc cggtgccccc gatctcatct tcgagcagga
      541 agtggaaaac ccggcgacgc ttgaagaaca gataaaaagt gtcaccgatc aagacgcttt
      601 tcgtcgttgg actgaagacg tggctccact attagagcag tcatcgaagc gcgagcttta
      661 tgaaattgtt tccaaatagt ggagtagggc ataggttttc acgtccaaat ctttcccagc
      721 cttcatgaag ctaacagcag tatcagcgca gacccgccgt atcatccacg accagcggaa
      781 ggatctgctt cgccgcgacg atcccgatct gaaaaggcta catgattagc gtgccgttgg
      841 ttttgcgccg cggctccggt tgctctcctg tgcagcggaa tccgggtcat cgggtcggag
      901 aaaatgcaaa gagatcacga gggcgacggt cagcgcggta agcagtccga gctcaatcat
      961 cgcgttgtga aaaatggcgt cattttcacg ggccatcttt ggcagttccg ccgttacgac
     1021 gaggatgcgg cgggtcacgg cgatcagccc gacgaccaaa aaaggtcccg gtcttagcac
     1081 atgctggcga aaggaaactt gcaccgtgta gagcacctcg acaaaaataa tgatgaggag
     1141 aatgcgatcg agaagcatga cgacgttttc cgacaattgc ccggcaagta ggtactggac
     1201 gaaataaatg atttctgcga tgagaagcgc accggcgatc ccggcaagca gtaaaccgag
     1261 accaacatag actgcatcct caacaaaatt aaaacttagt gagagccagc ggcgcatggc
     1321 gatccaacgt ggtctttctg tcatcgcggc ggacgagctg agtcgtgcgc caaccgacct
     1381 acattgcacc gtcgccgcaa gattagatcg ttccaacgta acaaagcggt agaaagctgt
     1441 gcgggcttca tttgaccacg cagacggcga ggcgctcgga ctgcgcgatc agttgctcag
     1501 cgatatgggc gtgaaatttt tccttgatcc acggataatc gttgtaaccg acaacgactg
     1561 tatggcaacg atactcatgg gctgctctga tgatctcaga gactaaatcg ccgcggtcat
     1621 tcaactgtaa aatgtgcgtc gttacttttt ccgcggggat attcgctgcg atgatttgcg
     1681 atttggcagc ttcgagcact gggacgactt gcgttccggc tcggtttttc cagctctctt
     1741 gctgggcagc ttgccgccgt tcgatttgct gctccctgct cgggtcttcc gcgccaggcg
     1801 actccatcag ttgcggcggt aatggcctca atgcgtgaaa tagatgaatg tcaacatcgg
     1861 cacgatcggc tacgaaggca gcaacgtatt ccacggcccg cttggcccca tcgtgatcat
     1921 ccacagcgat cagtatgttt tgaactggca acatggattc agtcttgcgc aactgctatg
     1981 ccacaaagca gttgtacctt gcaaatcctg ccgagttcct tcgcacaaat gttcctggtt
     2041 tcgacacagc gctagaccac acccgtcccg aatacaagac acgacggcga gagcaggcta
     2101 atattggtct cttccttgca gcctttagaa aatatctttg gaaaaggggg aaatatggcc
     2161 tacgcggaat ataaagcgaa aaagtttcaa ctcagcgggt tggaaggaat ttccgataag
     2221 accttggaaa tgcattttgg tctttatgag ggttacgtta aaaacaccaa tttattgatg
     2281 gagcaaatct ccgcaatcag taggaaaaaa caagcatcgg ggcaagaccc ggcttacgct
     2341 gaactcacac gccggctcgg ctttgaattc ggtggcatgg tattacacga atattatttt
     2401 gaaaacctag cctcaaaggg caaagggagt ccgtcgggtg attttaaaaa ggcgatcgat
     2461 gagagcttcg acggttttga aaattggcag caagcgttta ccgcagttgg gggtatgcgc
     2521 ggcgtgggct gggcgatttt atatcaggat cccgtgacag agcggctttc gaatcactgg
     2581 atagcgctgc atcaggatgg agtgccttcg ggcttcaagc caattctcgt catggatgtg
     2641 tgggagcatg cgtttattct cgactacaag cccgccgacc gcggtaagta tatcaacgcc
     2701 ttttttaaca atgtaaattg ggatgcggtt cagcggcggc tgactaatcc tgctgccgta
     2761 cgcgctgttg cataaccttc gcatcgagag ccgtcggccg gtaccgtttc ggccgacggc
     2821 tcaaatacag cttcagtgga tcctcaagga gtctggcagc ggttttcttc gtcagttccg
     2881 aaaatcttca aacaccgttc gagatgtctt gatttggcgc cggtcgtgta ccaagattga
     2941 gatagacacg tcatcccggc gacggacaat tgttccgtca cactgttcat
//
LOCUS       DASULZ010000659         6399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_784403, whole genome shotgun sequence.
ACCESSION   DASULZ010000659 DASULZ010000000
VERSION     DASULZ010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6399
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784403"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="VFY96_13680"
     CDS             complement(<1..1077)
                     /locus_tag="VFY96_13680"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5607568.1"
                     /translation="MKKTGIFIFFILFVWSTTVQAQTVDELRTAIQSSSDAPTKAGLY
                     KRLGDLFVQQDDLEQAADAFSSALALDRGNFSPRERLQMAIYMSWADRLNDSKDELQK
                     LLAEDPKNIAARTHLARVLSWSGELNAAINQADIVLRDSPDHKEALLVKADALQWQGR
                     YLEAIPIYGAILARENDFDARAGLSRSLLALGDRTSAIEGLRSLEPANARQKREIARL
                     SEAIERETRPALDARYNYYTDSDRNKLHRYSLAGSGWLGNQRYGLDYRHTDASDPTRH
                     NRGDDFLVSLYSRLTDRFSAGAGLGFTQLGDGNTSTFPTGNFRVDARLFAGRAGANVT
                     REVLTDTAELIENRIRMTNVGLYIA"
     gene            complement(1263..2711)
                     /locus_tag="VFY96_13685"
     CDS             complement(1263..2711)
                     /locus_tag="VFY96_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5607569.1"
                     /translation="MDAKNSMDDSTLQQSDYPYPRSGTLQREIEIPGQTFDEPVAGVT
                     PQPASPEKRKLYLPVKGKFFISTAFACVWISLSWFLAQHWLAELAQVVGEMPAAVIVF
                     FIALLPGFLNAHILSSVLLDRPPKLQTDMKFPAISLLIAAYNEEENISETIRGIKNQD
                     YPAKIEIIVVDDGSIDNTVSMLHSFNLPNLKIIRSNHGGKARALNEGLQHVTGDIVVC
                     IDADTFLHPQALRRIIARFLTAPADTAAVAGCILVKNSRSTFMTRLQEWDYFTGIASA
                     KRQQSLYQGTLVAQGAFSAFRTKIVKAHDGWPSVIGEDIVLTWSLINSGWRIGFEPTA
                     IGFTSAPETIQGFYRQRKRWARGMIEGLKRYGHLVWKSSRLSAFFVGVDFIIPFIDLF
                     YAFVFLPGIILAFFGHYYVVGPQTLMVIPLAFLIVLIMYKKQKAVFNELGLRVRQNLV
                     GFLVYMLIYQALMSPICVIGYGQEIFGMTKRW"
     gene            3090..3707
                     /locus_tag="VFY96_13690"
     CDS             3090..3707
                     /locus_tag="VFY96_13690"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEX5607570.1"
                     /translation="MILEMRTYVLKPGMTHNFVERFAEGLTARLQFSKLLGLWLSEVG
                     GLNRVVHVWPYESFEDRERVGTEARKTGKWPPKTQEFTVTQENKFIQPAPFSPPLPER
                     KLGEVYEFRTYTYQPGTMPTVLERFGKVIPARTKVSPLVFAGQTIVGPLNQYVHVWAY
                     KDAGERERLRAEATKSVEGWPPATREFLVMQENMIMTPAPCAPFK"
     gene            3887..4990
                     /locus_tag="VFY96_13695"
     CDS             3887..4990
                     /locus_tag="VFY96_13695"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEX5607571.1"
                     /translation="MLYKSIVRPLLFHKDPESSHEFILALMARAEFLSPLVEKGYRID
                     DQRLAVNLGPLKFTNPVGLAGGFDKNARAPKMIAAFGFGFMEVGAITAQAQPGNPKPR
                     LYRLPEDCALINRLGFNNEGAAAIAIKLAKRRARGGLPKIPLGLNLGRTKIVETKDAV
                     ADFLSCFVTLFPHGDFFTLNVSSPNTPQLRDLQEKTLLRELLSAVQERNRELAASAQI
                     APKPVFVKIAPDMEFAQVDEIVEVAAAAKLTGIVATNATAFMREGLKSRCASEPGGLS
                     GRPITAVVTSFIAHIYQTTRGSLPIMGVGGIFDAQDAYDKIRAGANAVQIYTGWIYEG
                     PGVVKRINTGLLRLLERDGLKRITDAIGVDVRN"
     gene            5047..5538
                     /locus_tag="VFY96_13700"
     CDS             5047..5538
                     /locus_tag="VFY96_13700"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEX5607572.1"
                     /translation="MSRSFRIEVAKDYFNFASAHFLIFSNGQRESLHGHNYQVSVVME
                     GELDPAGVVLDFISFKPLVKRICDGLDHRTLIQTASPVITLHQRPNEIELRYKQQKII
                     LPRQDVLLLPLINTSTELLAEYVAGQIRRKVRRNFPKSQIRYLEVGVEEARGQRGFFR
                     GEF"
     gene            complement(5637..>6399)
                     /locus_tag="VFY96_13705"
     CDS             complement(5637..>6399)
                     /locus_tag="VFY96_13705"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5607573.1"
                     /translation="FEVWSDGKAPPYSPMTTSLKPGTPDLLGISWSRYGGRTGVWRFM
                     RIFEDVEIAATVCLNARCAELFPEAVKELHRRGHEIAGHSYTQDLVLAYVSPAEEHEI
                     IQRCKRIIMDAVDTAPVGWFSPVAAPTEHTAEFLAEENFIWHGDYNDTDLPYILETAK
                     GKLVAIPHSDFTDNRTLRSSPRDFYNVYKDTFDYLYRYEAPSLINLTVHAHFGGRPLM
                     SAMLHELLKYMRGYPDVWFARHEEVARWTIDQAET"
BASE COUNT         1553 a         1649 c         1751 g         1446 t
ORIGIN      
        1 cgcgatatac aagcccacgt tcgtcattcg aatgcgattc tcgatcagtt ccgctgtgtc
       61 cgtcagcact tctcgtgtta cattcgcgcc cgctctgccc gcgaacaatc tcgcatctac
      121 gcgaaaattg ccggtgggaa aggtggacgt attcccatct ccgagctgag tgaaaccgag
      181 gcccgcgcca gccgaaaacc tgtcggtcag gcgcgaataa agcgagacca ggaaatcatc
      241 gcctcgatta tgtcgcgtcg ggtcgctagc gtccgtgtgg cggtaatcaa gaccatacct
      301 ctggttgcct agccaaccgc tgcccgcaag tgagtatcga tgcaatttat tccgatccga
      361 atcggtgtag taattgtagc gagcatcgag ggccggacgc gtttcccgtt caatggcctc
      421 gctcaatcga gctatctcac gtttctgtcg agcgttagcc ggttcgagtg agcgtaagcc
      481 ttctatggcg ctggtgcgat cgcccagcgc caagagcgac cgggatagcc cggcgcgcgc
      541 gtcgaagtca ttttcccgag ctaatatggc tccatagatt ggaatggctt cgagatatcg
      601 tccctgccat tgcaacgcat cagctttgac cagcagcgcc tctttatgat cgggggagtc
      661 ccgcaagaca atgtccgcct ggttgatcgc tgcgttgagc tcgcctgacc aagagaggac
      721 acgagcgaga tgcgtgcgcg ctgcaatatt cttggggtct tccgctaaca acttctgtaa
      781 ttcgtctttg gagtcattca agcgatccgc ccaagacata tagatggcca tctggagccg
      841 ttcgcgcggt gaaaaattgc cccgatcgag cgctaatgcg ctggaaaacg catcggccgc
      901 ttgttcgaga tcatcttgct gaacaaaaag atcacccagc cgtttgtaaa gcccggcttt
      961 tgtcggggca tcggaactcg actgaatcgc tgtgcgcagc tcatcaacgg tctgagcctg
     1021 cacggttgtc gaccaaacga aaagaatgaa gaaaataaaa attcctgttt ttttcatagc
     1081 gcttcgacgc aaaactccga gaaatggccg gaatcataac acaggagagt tctgaaaaat
     1141 agaaaatttg cgacttcggt tgaaagtcgc gccaaaaaaa cgccaggaag gctgaaaaca
     1201 gccggtggca gatactcaga tctaattgat cggcgagaag ctcgagagaa gaaacgccgt
     1261 acttaccagc gcttcgtcat tccgaaaatt tcttgcccgt agccaatgac gcatatcggt
     1321 gacatcagcg cttgatagat gagcatgtaa accaaaaaac caactaagtt ctgtcgtacg
     1381 cggagaccga gctcattgaa gaccgccttt tgttttttgt acatgatcaa cacgataaga
     1441 aacgccaaag gtattaccat caaagtctgc ggcccgacga catagtaatg cccgaagaaa
     1501 gccaaaatga tgccgggaag aaacacgaaa gcatagaaaa gatcgataaa gggaataata
     1561 aagtcgacgc cgacgaagaa tgccgacaaa cgtgaactct tccagaccag atgaccgtag
     1621 cgttttagac cctcaatcat accgcgcgcc caacgtttac gttggcggta aaatccttga
     1681 atcgtttccg gcgcgctagt gaaaccgatc gcggtgggct cgaatcctat gcgccagcca
     1741 ctgttgatca gcgaccatgt gagtacgata tcttcaccga tcaccgacgg ccatccatcg
     1801 tgcgctttga cgatcttggt acgaaaagca ctgaacgcgc cctgggcgac gagagttccc
     1861 tgatagagcg attgctgacg cttggcagag gcgattccag tgaagtaatc ccattcctgc
     1921 agccgggtca taaacgtcga gcgcgaattc ttcaccagga tacagccggc aactgccgca
     1981 gtatcggcag gggcggtcag gaagcgcgcg atgatacgcc ggagagcttg cggatgaaga
     2041 aatgtgtcgg catcgataca aacaacgatg tctcccgtta catgctgcag tccctcgttg
     2101 agagctctgg ctttgccacc atggttcgac cgaataatct tcagattggg taaattgaac
     2161 gagtgaagca tggagacggt attgtcgatg gagccgtcat caaccacgat gatctcgatc
     2221 ttcgccgggt agtcctggtt tttgatgcct cgaatcgttt cagagatgtt ttcttcttcg
     2281 ttgtaagccg caatgaggag actgattgcg ggaaatttca tatcggtttg gagtttgggc
     2341 ggacggtcaa gaaggacact cgataaaata tgcgcgttca gaaatccggg cagcagggcg
     2401 atgaaaaaaa cgatcacggc tgccggcatt tcgccaacca cctgagcgag ctcggcgagc
     2461 cagtgttgtg cgaggaacca agaaagcgaa atccagacac acgcaaaagc tgttgagata
     2521 aaaaacttgc ctttgaccgg cagatacagc ttgcgttttt ccggtgaagc gggttgtggt
     2581 gtaactccgg cgacgggctc atcgaatgtt tggccgggaa tttcgatttc tctctggagc
     2641 gtgccggagc gtggataagg atagtcgctc tgctgcagtg tggaatcatc catcgaattt
     2701 ttggcgtcca ttttcaactt tctctaatga aggaaatgat cgggtttact aaacaggctg
     2761 cgctcaggag caacatgtgt tccatcgtcg gacgcggtag cttgtggcac tttggagttg
     2821 ttgggagagc gaggtatctt ggtcgggatg acaaggatgg caagcaatca agctttgttg
     2881 acaagtgggt cgattcgcat ttcggaatgc tctgatggct gaccaatgag ttgactagcc
     2941 ttgcttccaa ggcgaaccac gcgttacgac agggaagatc gagacgcaaa ttcaaaccgc
     3001 caagatattt ttcgaacaac agagctgctg ttgcatttac tcagcgcttt atgggaaagg
     3061 ataggaaaca caatcaaagg aggtaggcga tgattctcga gatgcgaacc tatgtgttga
     3121 aaccggggat gacccataac tttgtcgaac ggtttgccga agggctcacg gcgagattgc
     3181 agttttcaaa attgctcggc ttatggcttt ccgaagtagg cgggttgaat cgagtcgttc
     3241 acgtctggcc gtatgaaagc ttcgaagatc gcgagcgcgt cggcaccgaa gcacgaaaga
     3301 ccggcaagtg gccgcccaaa acgcaggagt tcaccgtcac gcaggaaaac aaattcattc
     3361 agcccgcgcc gttttcacca ccgcttccgg agcggaagct aggcgaagtt tacgagttcc
     3421 gcacctacac gtaccagccg ggcacgatgc cgaccgtgct ggaacgtttt gggaaagtga
     3481 tcccagcgcg gacgaaggtt tcgccgctgg tattcgccgg gcaaacgatc gtcgggccgc
     3541 tgaaccaata cgttcatgtt tgggcctata aggatgcggg cgagcgcgaa cgtttgcgcg
     3601 ccgaggcaac caagagtgtc gaaggatggc cgccggcaac tcgagaattt ctggtcatgc
     3661 aagaaaatat gatcatgact cctgcgccgt gcgctccgtt caaataagct cgattcacca
     3721 gccgatttaa aattttagcg cgggccattg tcagttgcgt cggagagagg gagagcttct
     3781 gcagtctccc tctccgtttg tttggcacga accgtttacc cacagattct attcacgccg
     3841 gaaagaacca gggttcgatg cgtcttacga gtagctttcg agctctatgc tttacaaatc
     3901 gattgttcgt ccgctgttgt ttcataagga tccggaatcc agccacgagt tcatcctcgc
     3961 gctgatggcg cgagcggagt ttttgtcgcc gctggtcgag aagggttatc ggattgatga
     4021 ccagcgtctg gcggtaaatc tcggaccgct aaagtttacc aatcccgtcg gccttgccgg
     4081 cggttttgat aaaaacgcgc gcgctccgaa aatgattgct gcattcggtt tcggtttcat
     4141 ggaagtcggc gcgatcaccg cccaagcaca gcctggcaac cccaagccgc ggctttatcg
     4201 ccttccggaa gactgcgcat tgatcaatcg gctgggcttt aacaacgagg gcgccgccgc
     4261 cattgcgatc aagcttgcca agcggcgtgc gcgcggcggc ttgccgaaaa ttccgcttgg
     4321 cctaaacctg ggtcgcacaa agatcgtgga gaccaaagac gcggtggcgg attttctcag
     4381 ttgctttgta acccttttcc cgcatggcga cttttttacc ctgaacgtca gttcgccgaa
     4441 cacaccccag ctccgcgatt tgcaggagaa gacgctcttg cgtgaattat tatccgcagt
     4501 ccaggaaaga aatcgcgaac tcgctgcgag cgcgcaaata gctcccaaac cggtcttcgt
     4561 caaaatcgcg cccgacatgg aattcgcgca agtagatgaa atcgtcgagg ttgcggcggc
     4621 ggccaaatta accggaatcg tcgcgaccaa cgcgacggca ttcatgcgcg aagggcttaa
     4681 atcgcgctgc gcttccgagc caggcggctt gagcggcagg ccgatcacgg cagttgtcac
     4741 gtcgttcatc gcgcatattt atcaaacaac ccgtggcagt ttgccgatca tgggtgtcgg
     4801 tggcatcttc gatgcccaag acgcttacga taagatcaga gccggcgcca atgcggtgca
     4861 aatctacacg ggttggattt acgaaggccc cggcgtagtc aagcgcatca atacaggact
     4921 gctccggttg ctcgagcgtg atggtctcaa acgaatcact gatgcgatcg gcgtggatgt
     4981 aagaaattaa atggtttgcc agatcacccc gttgaaagcc tcagtggttt tggctaacgt
     5041 ccgacaatgt cgcgatcatt taggatcgaa gtcgccaaag attacttcaa ctttgcttcc
     5101 gcgcatttcc tgattttcag caacggccaa cgagaatcgc tgcacggaca caattaccaa
     5161 gtgtcggttg tcatggaagg ggaacttgat ccggcgggcg tcgttctcga ttttatatcc
     5221 ttcaaaccgc tagtgaagcg catttgcgac ggtctggatc accgaacgct gattcagact
     5281 gctagtccgg tgattacgct gcaccagcgg cccaatgaga tcgagctgcg ttacaaacag
     5341 caaaaaatta ttttgccacg gcaggatgta ctattgctgc cactgatcaa tacgagtacg
     5401 gaactgctcg ccgagtatgt tgccggtcaa atccgccgaa aagtccgtcg gaactttcct
     5461 aagagccaaa ttcgctacct ggaagtaggc gtggaggaag cgcgaggcca gcggggattt
     5521 tttcgcggag agttctaacc ggctgtcgac cggtcaatcc aacatcgccg ccctggggat
     5581 ctggtttgtc aaaattgtgt tgagtatcgg cccggcgata gcgaattgct tgtgagctag
     5641 gtctccgcct ggtcgatggt ccagcgcgcg acctcctcat ggcgcgcgaa ccaaacatca
     5701 ggatatccgc gcatgtactt tagaagctca tgcagcatcg ctgacatgag cggtcggccg
     5761 ccgaaatgcg catgcacagt aagattgatc agtgatggag cttcatatcg atagaggtag
     5821 tcaaaagtat ccttgtagac gttgtaaaaa tcgcgcggac tggaacgtag tgttcgatta
     5881 tcggtaaagt cactgtgcgg gatggcaacc aatttgccct ttgcggtttc taaaatgtag
     5941 ggcagatccg tgtcattgta gtcgccgtgc cagataaaat tttcctccgc taaaaattct
     6001 gccgtatgct cagtaggtgc ggcgactggg ctgaaccagc cgaccggcgc cgtatctacc
     6061 gcatccataa tgatccgctt acaacgctga atgatttcgt gttcctccgc cggggaaaca
     6121 taggccagca cgagatcctg agtgtacgaa tggcctgcga tttcatgacc gcgtctgtgc
     6181 agttccttta ccgcttcagg aaacagttca gcgcacctgg cgttgagaca aacggttgcg
     6241 gcgatctcta cgtcttcgaa gattctcatg aatcgccaga ccccagttct gccgccgtag
     6301 cgtgaccagg agatacccaa aagatcgggc gttcccggct tgagcgaagt cgtcatcgga
     6361 gaatagggag gcgctttgcc gtcggaccag acctcgaac
//
LOCUS       DASULZ010000660         2764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1394989, whole genome shotgun sequence.
ACCESSION   DASULZ010000660 DASULZ010000000
VERSION     DASULZ010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394989"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..1147)
                     /gene="hemH"
                     /locus_tag="VFY96_13710"
     CDS             complement(203..1147)
                     /gene="hemH"
                     /locus_tag="VFY96_13710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX5607574.1"
                     /translation="MSKMYDAILLVAFGGPSSPEEIRPFLMRVTKGLRIPPERIEEVA
                     RHYEAVGGKSPLNEITFRQAAALRGALAAAGIRIPVYVGFRNAAPFFVDTLKQMRAEG
                     IQHGLGFILSSHQTEASWQRYHKNIADARAKIGGQAPQVEYCPGWHDHPLFIQSWAER
                     IEAAFAQLPAEWRQSTPIVFSAHSVPSAMAAQSPYEVQLQETARLIARSLNCSHWSLA
                     YQSRSGRPGDPWLEPDVGNVMQRLANEGHTAVVFAPIGFVSDHVEVLYDLDIEARKIA
                     QNLSMRFYRVSCPNDDPIFIRMIADVVVQTLNRAEHRT"
     gene            complement(1452..2489)
                     /gene="hemE"
                     /locus_tag="VFY96_13715"
     CDS             complement(1452..2489)
                     /gene="hemE"
                     /locus_tag="VFY96_13715"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX5607575.1"
                     /translation="MIAKTTHPFLAACRREESPYTPVWLMRQAGRYMEEYRKLRAEHD
                     FLELCKNSELASEITIAPVERLAVDAAILFADILLVLEPMGIGLEYAKNGGPVIHHPV
                     RSGKDVDALKEFNPHEELVFVYETVKRTSKALKNKVPLIGFAGAPFTLASYLIEGGGS
                     RNYVHTKKFFYSAPEAWKRLMERLAKVVTDYLNCQIAAGADAVQLFDSWAGCLTPADY
                     EHFVLPYSKAVIDGITPGTPVIHFSTGTAGLLKQIRAAGGDVIGLDWRVNLDEAWKTV
                     GHDVAIQGNLDPVALFAAPKEIKERAGEIVRRAEGRAGHIFNLGHGVLPETPVDHVIA
                     LVDAVHELSSR"
     gene            complement(2530..>2764)
                     /locus_tag="VFY96_13720"
     CDS             complement(2530..>2764)
                     /locus_tag="VFY96_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607576.1"
                     /translation="EIFETVSALLRQNILRGRAVRLLGVSASALQNSGWQEPLFNFER
                     RKSLERLYKGIDDLRRKYGEQSIGVASPRHRAG"
BASE COUNT          595 a          793 c          764 g          612 t
ORIGIN      
        1 ccgatttttt cggttgcaat cgaaccaccg agacgctgcg aagcttcgag caggatcaga
       61 tcgatggata aagatttttt tttaacaagc tcaacgaccc ggtgtgctgc ggcgaggcca
      121 gcgatgccgc ccccgatgac aacgatccgc ttcatgatcg aggatcgatg atggacgatc
      181 gaagattgcg atccaccatc ttctatgttc gatgttcggc gcggtttagt gtttgtacga
      241 caacgtcggc gatcattcga atgaatatcg gatcgtcatt ggggcagctc acacgataaa
      301 atcgcatgct gaggttttgc gcaattttcc gtgcctcgat atcgagatca tacaaaacct
      361 ccacgtggtc ggagacaaag ccgatgggag caaagacgac cgcggtgtgc ccctcgttcg
      421 cgagtctctg catgacgttg ccgacatccg gttcgagcca aggatcgccg ggcctgccgc
      481 tccggctttg atatgcaagg ctccagtgcg aacagttgag gctcctcgcg atcaatcgtg
      541 cggtttcttg cagctgtacc tcgtatggtg attgcgcagc catcgcgctg ggcacgctgt
      601 gcgcgctgaa aacgatcggc gtggactgtc tccactcggc tggcagctga gcgaaggctg
      661 cctcgattcg ttccgcccag ctttggatga acagcggatg gtcgtgccag cccggacaat
      721 attcgacttg tggcgcctga ccaccgattt tcgcacgggc atcggcgatg tttttgtgat
      781 agcgctgcca actcgcctcg gtttgatgcg acgatagaat gaaacccaat ccgtgctgta
      841 tgccctcggc gcgcatttgc tttagcgtgt cgacaaaaaa tggcgcggcg ttccgaaacc
      901 cgacgtaaac cgggatccgg atacccgctg cggctagagc tcctcgcaat gcggcagctt
      961 ggcggaatgt gatttcgtta agcggcgatt taccgcccac ggcttcgtag tgacgcgcga
     1021 cttcctcgat ccgctccggc ggaattcgaa ggcctttggt gacacgcatg agaaaaggtc
     1081 ggatttcttc aggcgaactc ggaccgccga aagcaacgag caaaatcgca tcatacattt
     1141 tagacattat gatgaattgc tctcggcgtc ccaaaacatg ccaatgtcat cctgaggcga
     1201 acgccgaagg atctcgtcgg ctgggaacaa cgcgtgacgc gtcgcagcgc cggactccgt
     1261 gaaaacttcg cttgtccttc gcgatgctca ggttgcccta gtacctatcc tccgataggg
     1321 cagcgctccg cgctacctac tcaggttatt ggatacgccg atttcccgag tagcgcgaag
     1381 cgcgtatcga gggaccgccg gcgtttcgca acccctattg cgctgcatca cgaactgcca
     1441 gttactctcg ttcaccgcga actcagctcg tgcacggcat cgacgagagc aatcacgtga
     1501 tccaccggtg tttcaggaag aacgccgtga cccaagttga agatatgacc cgctcgcccc
     1561 tcagcgcggc ggacaatttc accggcgcgt tctttgatct ccttgggagc tgcgaacagc
     1621 gccaccggat cgagattgcc ctgaatcgcg acatcgtgac caaccgtttt ccacgcttcg
     1681 tcaaggttca cccgccaatc gagcccgatg acatcgccgc ctgcagcgcg aatttgtttc
     1741 agtaagcccg cggtgccggt gctgaaatga atcaccggag tcccgggtgt gatcccgtca
     1801 atgaccgctt tgctgtaggg cagcacgaag tgctcgtaat ccgcaggcgt aagacaaccc
     1861 gcccagctgt caaagagttg taccgcatcg gcgcccgcgg caatttggca gttcaaataa
     1921 tcggtcacca ctttcgcgag ccgctccatc aatcttttcc atgcttccgg cgcgctgtaa
     1981 aaaaactttt tagtatgaac gtagttgcgt gagccaccac cctcgatcaa gtacgatgcg
     2041 agagtgaacg gcgcgccggc aaagccgatg aggggcactt tatttttgag cgccttggaa
     2101 gtccttttga ccgtctcgta aacaaacaca agttcctcgt ggggattaaa ctccttgagc
     2161 gcatcgacat ctttaccgct gcgcaccgga tgatgaatca ccggtccgcc gtttttcgcg
     2221 tattccaatc caatccccat cggttcgagc acgagcagaa tatccgcgaa aagaatggcg
     2281 gcatcgacgg ccagacgttc gacgggcgcg atggtgattt cggatgcaag ctcggaattt
     2341 ttgcacaact cgagaaaatc gtgctcggcg cgaagcttgc gatactcctc catataccgg
     2401 ccagcctggc gcatcagcca aacaggagta taaggagatt cttcgcgccg gcaggcagcc
     2461 agaaacggat gagtcgtctt ggcgatcatc ggtgagagtt ttcgacccta tggtgctttc
     2521 gcggtaaaat caaccggcgc gatgacgtgg cgatgccacg ccgatggatt gctcgccata
     2581 cttgcggcgc agatcgtcga tgcctttgta aagtcgttcc aaagatttgc gccgttcaaa
     2641 attgaaaagt ggctcttgcc agccgctgtt ttgcagcgcg ctggcgctca cgccgagcag
     2701 acgcacggcg cgtccgcgca ggatgttttg ccgcaggagc gcgctgacgg tttcgaaaat
     2761 ctcc
//
LOCUS       DASULZ010000661         2396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_582698, whole genome shotgun sequence.
ACCESSION   DASULZ010000661 DASULZ010000000
VERSION     DASULZ010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582698"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /locus_tag="VFY96_13725"
     CDS             complement(<1..1111)
                     /locus_tag="VFY96_13725"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5607577.1"
                     /translation="MIKSPDETAVSRRSARLILLSVCIGQTIVGVDQRAITVALPTLT
                     ETFHTAFTTIQWTVLVYDLVLIGLIITMGRLGDLYGRRRFYSIGFLIFVTASAICGLS
                     QTAGQLILFRAVQALGGSMIAANGRAIVSVNLPREERGRALGLTSTAFHIGFLTGPSL
                     GGFLIDTIGWRWIFYINLPFSLCGAYLAWKVVPETRSKEKTGIDVPGAILLLLSNALF
                     IYAIDQLPRVGWRHPVFLSCLSFSLVGLILLLRTESRAAAPVLNLSMFRSRLFSAGII
                     SLFLVSGTLSAINFLLPFFLQNLLGYSPSQVGWIIVADSVIIMIMAPIAGSWSDRLGS
                     RLLCTIGCAVIVAAQFFLASLSTDASLIRIIFPLAV"
     gene            1372..1983
                     /locus_tag="VFY96_13730"
     CDS             1372..1983
                     /locus_tag="VFY96_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607578.1"
                     /translation="MFSDNQIIRGGIAQENAVLEKLFKVLFKVIDEIPSSAERSSTKA
                     VFRARKLTEEAALKAATISGALALPPGPLGWLTILPDLAAIWRLQAQLVADIAAVFGK
                     RGNLTEASIIYCLFRHAAAQAVRDLVTRMGERIVVQRISMRATENILQTIGVKLVYRV
                     ARRGFWRWMPAIGALAVAGYAYYDTVLVGQTALEFFSKDIDAS"
     gene            2038..>2396
                     /locus_tag="VFY96_13735"
     CDS             2038..>2396
                     /locus_tag="VFY96_13735"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5607579.1"
                     /translation="MEITEAREFLMHNHHGVLVARKRDGSLQMTLVSPVIGADGKVTI
                     TARESTYKVKNIRRNPQVSLLVYGEKFNGSNYIQIDGKAEVIPLPQAMDTVLEWHRQI
                     RGEPASWEEVRKKTLAEG"
BASE COUNT          601 a          641 c          722 g          432 t
ORIGIN      
        1 agaccgccaa cggaaaaata atccggatga gcgaggcatc cgtgctcaag gatgcgagaa
       61 aaaattgcgc ggcgacaatg accgcgcagc cgatcgtgca gagcaggcgc gaaccgaggc
      121 gatccgacca agagccggcg atgggcgcca tgatcataat gatcaccgaa tccgcgacga
      181 tgatccagcc gacttgcgat ggagaatagc cgagcaggtt ctgtaagaag aacggcagca
      241 gaaagttgat cgctgagagc gtgccagaaa ccagaaaaag gctgatgatg ccggcgctga
      301 acaaacgcga gcgaaacatg gaaaggttca aaaccggtgc ggccgctctt gactcggtgc
      361 gcaatagcag aattaaaccg acaagtgaaa atgacaaaca tgagagaaac accggatggc
      421 gccagccgac gcgcggcaac tggtcaatgg cgtaaatgaa cagagcattg ctgagaagca
      481 aaaggatcgc gcccggaacg tcgatgccgg ttttttcttt gctgcgggtc tcgggcacga
      541 ctttccaggc gagataggcg ccgcacaggc tgaacggcaa attgatataa aagatccagc
      601 gccagccgat ggtatcgatc aaaaagccgc cgagggacgg accggttaga aagccgatgt
      661 gaaatgccgt cgaagtcaac cccaacgcgc ggccgcgctc ttcacgcggc aaattgaccg
      721 aaacgatggc gcggccattg gccgcgatca tcgatccgcc aagcgcctgt acggcccgga
      781 acaaaatcaa ttgcccggcc gtttgggaca agccgcagat ggccgaagcc gtgacaaaaa
      841 tcaaaaaacc gatactgtaa aaacggcgcc ggccgtagag gtcgccgagc ctgcccatgg
      901 tgataatgag accgatcaag acgagatcgt agaccagaac cgtccattga atggtcgtga
      961 atgcggtatg aaatgtttcc gtgagcgtcg gcagggcgac ggtgatggca cgctgatcga
     1021 cgccgacgat cgtctggcca atgcagacgc tcagtaaaat aagccgcgcc gagcgccgcg
     1081 agacggcagt ttcatctggt gacttaatca atttgtagtt ctcgccgcag tcttgtgcag
     1141 tctagcacat cctcttgagc tcgcatccgg tggcggcgca tgcggaaagt aaatttcacc
     1201 aacggtcact acgatcgcga actcagcagt cctccgatga cggtttggtt tgccggatca
     1261 cggcattccc ccgggatgtt ccgattgccc ggcgaaaaat tttcttgcac cgagccgtgg
     1321 aaatcattta attttgtatc ccacgggggg aactctatgt cagagaaatc gatgttcagt
     1381 gacaatcaga ttatccgcgg aggtatcgcg caagaaaacg cggtgctcga aaagttattt
     1441 aaggttcttt tcaaggtgat cgacgaaatt ccgtcgagcg ccgagcgcag ctcgacaaaa
     1501 gcggtctttc gcgcccgcaa attgaccgag gaggctgcat tgaaggcagc cacgatctcc
     1561 ggcgcactgg cgctcccgcc tgggcccttg ggatggttga cgattttgcc ggatctcgcg
     1621 gcgatctggc ggctgcaagc ccagctcgtc gccgatatcg ccgcggtttt cggaaagaga
     1681 gggaacctca cggaggcgtc gattatctat tgtttgtttc ggcacgccgc cgcgcaggcg
     1741 gtgcgggatc tggtgacacg catgggggag aggatcgtgg tgcaacgcat ctcgatgcgg
     1801 gcgactgaaa acatcctgca aaccatcggc gtcaagctcg tctaccgggt tgcgcgccga
     1861 gggttctggc gctggatgcc ggcgatcggc gccctcgcag tcgccggata cgcctattac
     1921 gacaccgtgc tggtcggcca aacggcactc gaattcttct cgaaggatat cgacgcatca
     1981 taggtgagcg gccatcttat taggaatcga agccacaagc gaaggagcga ttcgttcatg
     2041 gaaattaccg aggcgcgaga gttcctaatg cacaaccacc atggcgttct ggtggcgcgc
     2101 aagcgtgacg gttcgttgca gatgacgcta gtctcaccgg taatcggcgc agatggcaag
     2161 gtgacgatca ccgcgcgcga aagcacctac aaagtcaaga acatcaggcg caatccgcaa
     2221 gtgtcgttgt tggtgtacgg agaaaaattc aacggctcca attatatcca gatcgacggc
     2281 aaggccgagg tcatcccgct gccgcaggca atggacaccg tgctggagtg gcaccgtcag
     2341 atccgcggcg agccggcgag ctgggaagag gtccgtaaga agaccctcgc cgaagg
//
LOCUS       DASULZ010000662         3721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_784759, whole genome shotgun sequence.
ACCESSION   DASULZ010000662 DASULZ010000000
VERSION     DASULZ010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784759"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VFY96_13740"
     CDS             complement(<1..816)
                     /locus_tag="VFY96_13740"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607580.1"
                     /translation="MKQRCLAFLSLCVLCAGQVSAADRVRIGYSSISGAYTPIWVAHE
                     GGYFAKEGLQDDIILIPSGTQLAQVTVAGEIDIGSLNGSSAIAAALQGADLKIIGNSG
                     NKMVFSLYVRPEIKTVEQLKGKKIGITRFGSAPDISVRYALRKYNINPDKDLTLIQLG
                     FMATVAAGLQGGSIEGGVVSPPTQFAVDKAGFKELISITDMNFAFPNPALVAVGSIIK
                     NRPDVITRFIRAYVRGTHRARTDPAFTYKAMAKYTKIEDSAVLQKAYEFYMSKV"
     gene            961..2001
                     /locus_tag="VFY96_13745"
     CDS             961..2001
                     /locus_tag="VFY96_13745"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX5607581.1"
                     /translation="MNTRMIVIGTGPIGGIIGGRLARAGHDVSFVDVDKEHVAAIRKN
                     GLQVDVPDGAFNVKVKIAFPEEIEGKYDIGLIAVRSNYTPDALRTVLPHLRDNGLLVS
                     MQNGINPPLLAEKVGVDRTIGMAIRMGCRKVAPGHVQTAIRGHLYIGHLHGKITPQLE
                     DLQRTLNHVMDTEITDNILGVLWSKLTYTCLGYYGSLADASLGESCAGEACRRNLTNF
                     FAEVVAVGKASGARFISLAEYNPLDFHPSLPFEKRLAAVNQFAKSWKIDDRKGPLRQI
                     QKGIKTEVDFTLAYVVREGEKRQLAMPLCRKVLAMIHELEQGTRNLGLQNYDELSSVS
                     ADESEIVSHRIE"
     gene            complement(2054..2839)
                     /locus_tag="VFY96_13750"
     CDS             complement(2054..2839)
                     /locus_tag="VFY96_13750"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5607582.1"
                     /translation="MSLSIVAPIFGQSSPRAFAPKSGQPGKDVVWVPNPDVMVQKMLD
                     MAHVTRDDYVVDLGSGDGRNVIAAAKRGARAHGIEYDPDLVELAKRLAAKEGVRDRAT
                     FERGDVFVTDFSKASVIILFLTPEMNIRLRPKLLSLKPGSRVVANYFAIGDWNPDEIA
                     TNIEPCERFCTARLWIVPAQIAGRWQMPQGELTLTQNYQTFSGTLRAADGTFPVRGKL
                     RGAEIFFGNQKSQYTGRVDGDVIDGIARSAGIDHKFRANRLKP"
     gene            complement(2933..3706)
                     /locus_tag="VFY96_13755"
     CDS             complement(2933..3706)
                     /locus_tag="VFY96_13755"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5607583.1"
                     /translation="MFASSGAPAQSGKSEYQPQVGQEGKDVIWVPTPQALVDKMLDMA
                     KVTAKDYVIDLGSGDGRTVITAAKRGAKALGIEYNPDMVELSKRNAAKAGVSDKASFV
                     KADLFEADLSPATVITMFLLPDINLKLRPKILNLKPGTRIVSNSFTMGDWNADDTAVV
                     KEGCASYCTAYLWIVPAKVEGNWQLGDGTLTLKQTYQMVSGTLNRGSNAVQIANGKLD
                     GDRVTFNAGGALYTGRVTSNGMEGTISTGGEWKAVHGGR"
BASE COUNT          811 a         1071 c         1013 g          826 t
ORIGIN      
        1 aactttgctc atataaaact cataggcttt ctgcagcacg gcgctgtcct cgatcttggt
       61 gtacttcgcc atggccttgt acgtgaacgc cgggtcggtg cgggcgcggt gcgtaccccg
      121 cacataagcg cggataaaac gggtgatcac gtcgggccga tttttgatga tcgaaccgac
      181 cgccaccagc gccggattgg gaaaagcgaa attcatatcg gtgatgctga tcaactcttt
      241 gaaaccggcc ttgtcgacgg caaactgggt ggggggcgag accacaccac cttcgataga
      301 gccgccctgc agcccagcgg cgaccgtggc cataaatccc agctgaatca aagtcagatc
      361 tttgtcggga ttgatgttgt attttctgag cgcgtaacgc accgatatat ccggcgccga
      421 cccgaatcgg gtgatgccga tttttttgcc tttgagctgc tcgacagttt tgatctccgg
      481 acgtacatag agcgagaaga ccatcttgtt tcctgaattg ccaatgatct tgaggtcggc
      541 cccctgtaat gcggcggcga tcgccgagct gccgttgagg gacccgatat cgatctcgcc
      601 tgccacggtc acctgcgcga gctgggttcc gctcgggatc aggatgatgt cgtcctgcaa
      661 cccttctttg gcaaaatagc cgccctcatg cgccacccag attggcgtgt acgcaccgct
      721 gatggagctg tagccgatgc gaacccgatc ggcggcggag acttgaccgg cgcagagaac
      781 gcaaagactc aaaaatgcca agcaccgttg tttcatagct gcccctttcg atgacgtgag
      841 actgcatcca cagaaactat gttaccgacc tggtttgttc aagagttcgg cgccgcggtt
      901 atcagttgac gctattgcgc gcattcaata tgttggccgc gttcagcagc ggaggtagaa
      961 atgaacacgc gcatgattgt gatcggcacc ggcccgattg gcgggatcat tggcggcagg
     1021 ctggcgcgcg ccggtcacga tgtaagcttt gtcgatgtcg ataaagagca cgtcgccgcg
     1081 atccgtaaaa acggcctcca agtggacgtg cccgatggcg ccttcaacgt gaaagtgaag
     1141 attgcgtttc ccgaggagat cgaaggcaaa tacgatattg gattgatcgc cgtgcgctcg
     1201 aactatacac cggacgcgct gcgcacggtg ctgccgcatc ttagggataa cggtttactg
     1261 gtatcgatgc aaaacggcat caacccaccg ctgctcgcgg agaaagtggg cgtcgacagg
     1321 accattggca tggccattcg catggggtgt cgcaaagtcg cgcccggaca cgtgcagacg
     1381 gcgatccggg gtcatctcta tattggccat ttgcacggca agatcacgcc gcagctggaa
     1441 gatctccagc gcacgctgaa tcatgtcatg gatacggaaa tcaccgacaa cattctcgga
     1501 gtgctctgga gcaagctcac ttacacctgc ttgggatact acggctccct cgccgatgct
     1561 tccctgggag agagctgcgc tggcgaagcg tgccggcgca atctgacaaa cttttttgcc
     1621 gaggtcgtcg ccgtgggaaa ggctagcggt gcccgcttta tctcactcgc cgaatacaat
     1681 cccctggatt ttcatccgag ccttccgttc gaaaagcggc tcgctgccgt caatcaattc
     1741 gccaaatcct ggaaaatcga cgatcgcaaa ggcccgcttc ggcagattca aaaaggcatt
     1801 aaaaccgaag tcgacttcac cctcgcctac gttgtgcgcg aaggcgagaa acgacaactc
     1861 gccatgccgc tctgtcgaaa agttttggct atgattcatg agttggagca aggtacgaga
     1921 aatctcggct tgcagaatta cgacgagctc agctcagtca gcgctgacga aagtgaaatc
     1981 gtgtctcacc gaattgagtg agtttccgtc tttgtaaacg gcgccacgga gtgcctctta
     2041 ccgaagctga cgttcacggc ttcagacgat tcgcacgaaa tttgtgatcg atacccgcgc
     2101 ttctcgcgat gccgtcgatg acatcgccat ccacccgtcc ggtgtattgg ctcttttgat
     2161 tgccgaaaaa aatttctgct ccacgcaact tgcctctgac tggaaatgtc ccgtcggccg
     2221 ctcgcaaggt gccagaaaac gtctggtaat tttgcgttag cgttagctcg ccttggggca
     2281 tctgccatct gccggcaatc tgggccggca cgatccacaa acgtgccgtg cagaaacgct
     2341 cacaaggctc tatgtttgtg gcgatctcat cggggttcca atcgccgatg gcgaaatagt
     2401 ttgccaccac tcgggaaccc ggtttgaggc tcagcagttt cggccgcagg cgaatgttca
     2461 tttccggcgt caaaaataaa ataatcacgc tcgctttgga gaaatctgta acgaagacat
     2521 cgccgcgctc gaaagtcgct cgatcgcgaa ctccttcctt ggccgccagc cgcttcgcaa
     2581 gctcgacgag gtcgggatcg tattcgatac catgagcgcg agcgccgcgc ttcgccgcag
     2641 cgatgacgtt ccgcccatcg cccgacccga gatccacgac ataatcatcc cgtgtgacat
     2701 gcgccatatc gagcatcttc tgcaccatca catccgggtt gggcacccag acgacgtctt
     2761 taccgggctg cccggatttt ggcgcgaatg cgcgcggcga agattgtcca aaaattggcg
     2821 cgacgatgct cagcgacaag attataacga ttgcgccagt tgaaagtgcg cggatcacca
     2881 cccgtccttc aagggatcga cgcagcggga aaaatcacgc gggcaattgc acctatcggc
     2941 ccccatggac agctttccat tcgccgcccg tgctgatcgt cccctccatg ccattgctcg
     3001 taacccgacc cgtgtagagc gcgcctcccg cattgaaagt gacgcgatcg ccgtcgagct
     3061 tgccattggc gatttgaacg gcattactgc cgcggttgag cgttccagaa accatctgat
     3121 aggtttgctt caaggtgagc gtcccatcgc caagctgcca gtttccttct actttggccg
     3181 ggacaatcca gagatacgcg gtgcaatagg aggcgcagcc ctccttgacc accgccgtat
     3241 catccgcatt ccaatcaccc attgtgaatg agttggaaac aattcgcgtt ccgggcttta
     3301 agttaaggat tttcgggcgc agcttcaaat tgatatccgg cagcagaaac atggtgatca
     3361 ccgtcgccgg agaaagatca gcttcgaaca gatccgcctt gacgaagctc gctttgtcgc
     3421 tcactcctgc tttcgctgca ttgcgtttgg acagttcgac catatcggga ttgtattcga
     3481 tgccgagcgc tttagcgcct cgctttgcgg cggtaatcac ggttcggccg tcgccggatc
     3541 caagatcgat gacgtaatcc tttgccgtga ccttcgccat atcgagcatc ttgtcaacga
     3601 gcgcctgcgg cgtcggcacc caaatgacat ccttaccctc ttgaccgacc tgcggttgat
     3661 actcgctttt gccggattgc gccggtgcgc cgctgctcgc gaaaatgagt gaaagaaata
     3721 a
//
LOCUS       DASULZ010000663         6905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1088517, whole genome shotgun sequence.
ACCESSION   DASULZ010000663 DASULZ010000000
VERSION     DASULZ010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6905
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088517"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VFY96_13760"
     CDS             complement(<1..419)
                     /locus_tag="VFY96_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607584.1"
                     /translation="MFTKVLTTTAVATLGIVGLAIANGDQPTRFIVRIENITKPDTFT
                     ASNGVKWSLAFSPGTAIVHTEKAPVFSAGKKDRGKGLEAQAEDGDPSMLAKSLAGAKG
                     IKSVTVFNTPVGASTPGPITPGAVYEVTVSAVPGDRLS"
     gene            complement(659..2398)
                     /locus_tag="VFY96_13765"
     CDS             complement(659..2398)
                     /locus_tag="VFY96_13765"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX5607585.1"
                     /translation="MSFGKQEEAQSTASQIGIAIMLAALLLFAATFARAASIDEMLAK
                     RKPEANINLATKEGAQLVKGEWRYSDTKIVEIDFRAAGPDGQPGETSNKTYDFTPHAG
                     RADFDDSKWEVIDPTSLDKRRSAGKLAFNWYRMKITVPERIGDLDPTGSTLVFSTSLD
                     DYAEIWVDGEISRAAGQSGGSVIKGWNAENRLIIGRGVKAGQKIQLAIFGINGPISNP
                     PTNYIYMKYAKLEFHKVPAGPVAVVPQEVNVEVVRNDPAMDAVVPENPKIFKLAEGFQ
                     FTEGPVWVKPGYLLFSDPNANTIYKYNAADAKLSVFKVPSGYSGKDIGQYFQPGSNGL
                     TLDPQDRLTINEHGNRRVTRIEKDGRSTVLADHYEGKRLNSPNDLVYRSDGTLFFTDP
                     PFGLPKFYDDPRKELSYSGVFALYQGQLKLVSADLKGPNGIAFSPDEKYLYVGNWDPE
                     KKIIMRYEVGADGSLSNGQIFFDMTQAPGEDAIDGIKVDQRGNLYASGPGGLWIISPE
                     AKHLGTILAPKHPHNMAWGEDGKTLYLTAQGTLYRMPLKVAGIGAGTPVGNLSQAKQS
                     KFQRAEFHGQRTR"
     gene            complement(2597..3460)
                     /locus_tag="VFY96_13770"
     CDS             complement(2597..3460)
                     /locus_tag="VFY96_13770"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX5607586.1"
                     /translation="MKFRYHFVFLTLLLLPYHSLHAQLLEKKVLSLEAAEKVASAAAL
                     EANKRNARVVIVVVDDGGYPLVLKRLDDTQVASVEVGIGKARTAAIFRRPSRDFEEQI
                     KTGRVASLVLPGATPLQGGLPLIAGGKVVGAIGVSGNTPQEDEDIAKAGATAFSAMMT
                     SAQSSAVTYLPAKKVSQAFTQGMPLVRGMNYKIDASHRDEPGSVEVHTRDTDLIYMLE
                     GTATLVTGGTITDEKAIEPEEIRGRKSEGGETRIVSKGDVIVIPNGTPHWFKEVNGAI
                     NYYVVKVRSAN"
     gene            complement(3581..4081)
                     /locus_tag="VFY96_13775"
     CDS             complement(3581..4081)
                     /locus_tag="VFY96_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX5607587.1"
                     /translation="MKAIAIGTATLALTFGTLAAAHTQTIEKKALNLEGAKKVIAAAT
                     AEAKSKNAPGGAIAVVDEGGNLVAVERLDNTFAAGANISIGKARTAVLFKRPTKIFED
                     VINKGRTAMTTLNDFTPLQGGIPILVDGQIIGAVGVSGAATAQQDEELAIAGANALAT
                     SAAQAK"
     gene            complement(4273..4440)
                     /locus_tag="VFY96_13780"
     CDS             complement(4273..4440)
                     /locus_tag="VFY96_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607588.1"
                     /translation="MPRYEIRYRFRESVLTYLGRAYYETGNDPEARDVLQKAVGRNRN
                     DNLAKVYLGPN"
     gene            4445..6352
                     /locus_tag="VFY96_13785"
     CDS             4445..6352
                     /locus_tag="VFY96_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607589.1"
                     /translation="MVVRFEILDTKTGKRVTDKDGATIKLVLPHGEEIPGRWAIRGGA
                     AAMPDSAWMWDTPWDIPPDYPIGSFDYRITIAARDGRTAISLRQSRRRPPPTAAFGLS
                     TSVSIVLRGFKIVQLLVLLAACGGWIALYAGGGPPAIAVADSSRTAQNERFQSAAQAP
                     VGQELAPALKLLKVEPVKGYVSDPFTVSGEGFAAGAAVEFFWSTVEPSYLTKVLPDNL
                     EYHERKYDEKRVVLGLAQVDAQGRVNAKFTAPEDFGEVHDVFAVVEGRDLARGGFRIL
                     RSASLTPKTGSVGTPITVTVNGIGWHGFEQFMALRYDNKYTGEISAVTTKGSAVFQIR
                     AAGEPGKHIVQLNNSTAYSPGAYLNAQQSPQAYIYAHLDNQQEFRFTFDITTDNEIPP
                     DRIEWPEQGRVAVLSADAPRTTMSNKSVVGAATLTPASGTVNTQATVSAKNLPSNTQV
                     ELQFITARGNRMTPSGWNLDYLPVGKARTAGDGSLKQTVTMPDDLGGWHIIRIATEDR
                     VLAETPYYLEQSLVQVSPRRVKVGEVFTVQIKGVGWTELDNTLAVTYDNAVLGYACGF
                     NTNGDVTVNMVGTGRPGIHLVDFYPAIYRGRDRVPWNYQTPFLTYARDFPALPHGYRL
                     PAYRLAIEVIE"
     gene            complement(6621..>6905)
                     /locus_tag="VFY96_13790"
     CDS             complement(6621..>6905)
                     /locus_tag="VFY96_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607590.1"
                     /translation="GLSTILDGSRRHELMNMERSIDEGSFICGSPETVADQIKKIASQ
                     AGADTFLGEFTFGELTQKQVLSSLKLFTAQVQPELRKFEIDALSFPKASL"
BASE COUNT         1432 a         1861 c         1966 g         1646 t
ORIGIN      
        1 gacaatcggt ctccaggcac ggctgaaact gtcacttcat agacggcgcc gggagtaatc
       61 gggccaggag tgctcgcgcc gacaggtgtg ttgaacacgg tcaccgactt tatccctttg
      121 gctccagcca gagatttggc gagcatgctg ggatcgccat cttcggcttg agcctccagt
      181 cctttaccgc ggtctttttt cccggcgcta aagaccggtg ccttctccgt gtgaacaatc
      241 gcggttccag gtgagaacgc gagcgaccat ttaacgccgt tggacgcagt aaatgtatcg
      301 ggcttggtga tgttctcgat ccgtacaata aacctggtcg gttgatcgcc gttagcgata
      361 gccaggccga cgatgcccag agttgcaacc gctgtggtcg taagaacttt ggtaaacact
      421 tgggtctcct cctctttatt ttcgctggtt tcagaatcct cagccggttc ttgcggtttg
      481 gatgcttgcc aagtcgttaa ggatgcaacc gctttggaag actacccgcg caagcgaaag
      541 gcgggaaccc tgacgaggcc gacatcgctc aataaaagat cttgcatcat gatcggctga
      601 gtcaaatttt tattgtcggt tctccggcgc gcgattgagc acgtttacgc tcgcgagttt
      661 accttgtcct ttggccatga aactccgctc gctgaaattt cgattgtttc gcttgagaca
      721 aattgccgac cggcgtgccg gctccaatcc cggcgacctt caacggcata cgatagagtg
      781 ttccttgcgc cgtcaaataa agggtttttc cgtcttcgcc ccatgccata ttgtgaggat
      841 gcttgggcgc gaggatcgtg ccgagatgct tcgcctcagg agaaataatc cacaatccgc
      901 caggacctga ggcatagagg ttgccgcgct ggtcgacctt gatgccgtcg atggcgtctt
      961 cccccggcgc ttgcgtcatg tcaaagaaaa tctggccatt gctcaatgag ccgtcggcgc
     1021 cgacttcgta acgcatgatg attttcttct ctggatccca gttgccgacg tagagatatt
     1081 tctcgtcagg ggagaaggcg atgccgttgg gtcccttgag atcggcgctg acgagtttta
     1141 gctgaccctg gtagagggcg aacacgccgc tgtaggaaag ctctttgcgc gggtcatcgt
     1201 aaaatttcgg caggccgaag ggcggatccg taaagaacaa agttccgtca gatcgataaa
     1261 ccagatcgtt ggggctgttg agacgctttc cctcgtaatg atcggcgagc accgtcgatc
     1321 ggccgtcctt ctcaatccga gtcacgcgac gattgccatg ctcgttgatc gttagccgat
     1381 cttgcggatc gagggtgaga ccgttggagc cgggctgaaa atattggccg atgtccttcc
     1441 cggaatagcc gctgggtact ttgaatacag aaagtttcgc gtccgccgca ttgtatttgt
     1501 aaatcgtgtt ggcgttgggg tcactgaaga gaagataacc gggtttaacc cataccggcc
     1561 cctcggtgaa ctgaaaacct tcggcaagct taaaaatctt tggattctcc ggcacgacgg
     1621 catccatcgc gggatcgttg cgaacgacct ccacgttcac ttcctgcgga accacggcca
     1681 ccggtcccgc tggaactttg tgaaattcca gcttggcata tttcatgtag atgtagttcg
     1741 tcggcgggtt ggagatcgga ccgttgatcc cgaaaatcgc caattgaatc ttttgccccg
     1801 ccttgacccc gcggccgata atcagccgat tttccgcgtt ccaacctttg atcaccgagc
     1861 cgccgctctg tcctgcggca cgagaaattt cgccgtcgac ccagatctcc gcataatcat
     1921 cgagggacgt cgaaaagacc agcgtcgacc cggtcggatc aagatctccg atccgttcgg
     1981 gaacggtaat tttcatgcga taccagttga acgcgagctt gccggcggag cgccgtttgt
     2041 cgagcgacgt cggatcgatc acttcccact ttgagtcgtc gaagtctgcg cggcccgcat
     2101 ggggcgtgaa gtcgtaggtt ttgttcgatg tctctcccgg ctgcccgtcc ggacccgcgg
     2161 ctctgaaatc gatttcgaca attttcgtat cgctgtagcg ccactctccc ttgacgagtt
     2221 gcgcgccttc tttagtggct agattgatat tcgcctcggg tttgcgtttg gcgagcatct
     2281 cgtcgatcga ggcggcgcgc gcaaaggtcg ccgcaaacaa gagcaaggcg gctagcatga
     2341 tcgcaatgcc gatctgactg gcggtacttt gcgcttcttc ttgcttgccg aaagacaatt
     2401 tcggagagcg aagatatcgc gatgttctcc tgaggcgata gccgaaggat ctgcttttcc
     2461 tatgcggtaa aggcgagatt attcgctgcc gctcagaatg agacgtttgc agttgttgcg
     2521 actgtgacgg agtttcagag cgatacagag gagctcgcgc tgaaccgcgt ggaatcattg
     2581 cttccatatt gtcacctcag ttggctgaac gtacctttac gacgtaatag ttgatcgcac
     2641 cgttgacttc cttgaaccag tgcggcgtac cgttgggaat tacgatgacg tcgcctttgc
     2701 tgacgatccg ggtctcgccg ccttcggatt ttctaccgcg gatttcttcc ggttcgatgg
     2761 ccttttcatc ggttatggtg ccgccggtaa ccaacgtcgc ggtgccttcc agcatataaa
     2821 tgagatctgt gtcgcgcgtg tggacttcga cactccccgg ctcgtcgcgg tgactagcat
     2881 cgatcttgta attcatgccg cgaacgagcg gcattccttg cgtaaaagcc tggctgactt
     2941 tctttgctgg cagataagtg acggccgacg actgcgccga agtcatcatg gccgagaacg
     3001 ccgtggcgcc ggcttttgcg atatcttcgt cttcttgcgg agtgttgccg ctgacgccga
     3061 tggcgcccac cacctttccg ccggcgatga ggggcaagcc gccttgcaaa ggcgttgcgc
     3121 cgggcaagac gagcgaggca acccggccgg tcttgatctg ctcctcgaaa tctctgcttg
     3181 gtcggcgaaa aatcgccgcc gtgcgtgcct tgccgatgcc gacctcgacg ctggcaactt
     3241 gcgtatcgtc gagccttttc agcacgagcg gataaccgcc atcatcgaca acgacgatga
     3301 ctactctggc gtttcttttg ttggcttcca gcgctgcagc ggaagcaact ttttctgcgg
     3361 cttcgaggct caagactttt ttctcgagaa gctgcgcgtg aagtgaatgg tatggcaaca
     3421 gcagcagcgt tagaaagacg aagtgatatc ggaatttcat gaaaggctcc ttgacatcgc
     3481 catggacgat ttactcgatc tacaaatggg gtctttttaa ccgtgaaaat caggaacttt
     3541 tgaacggatc aaaatcatct cgctcgaatt ctctcaggaa ttactttgct tgcgcggcgc
     3601 tggtcgctag agcgttcgcg ccggcgatgg caagctcttc atcttgctgc gctgtggctg
     3661 cgccgctgac gccgactgcg ccgataatct gaccatcgac gagaatcggg atgccgcctt
     3721 gaagcggtgt gaaatcgttc agggttgtca tcgctgtccg gcccttattg atcacatctt
     3781 cgaagatttt cgtcggacgt ttgaacagta cggcggtgcg cgctttgccg atcgaaatat
     3841 tcgcgccggc ggcgaaggtg ttgtccaacc gctcgacggc gaccaggttg ccgccttcgt
     3901 caacaaccgc aatagcgccg cccggagcat ttttgctctt ggcttcagcg gtagcggcag
     3961 cgatcacttt ctttgctcct tcgagattca gagctttctt ttcgatcgtt tgcgtgtgag
     4021 ccgccgctag tgtgccgaat gtgagcgcca gagtcgctgt gccgattgct atcgctttca
     4081 ttttcgcctc cagattcaaa aaaggttcca gttatttgcc gattagatgt ttaggcgcgg
     4141 taaagagatg cgagcggaaa gttgtagtga tcggttgagg cgtagagcga ccgcactgga
     4201 tcgttagttt gccaggtcgc aaaaacgagt tcgaaacttg ttacgagaaa agatgcgagt
     4261 ggaccgaagg cgttagtttg gaccgaggta gacttttgcg agattatcgt tacggtttct
     4321 gccgacggct ttttgcaaga cgtcgcgtgc ttccgggtcg ttgccggttt catagtaagc
     4381 gcggccaaga taggtcaata cgctttcgcg aaatcgatag cgaatttcgt aacgcggcat
     4441 gcggatggtc gtgcgctttg agattctgga tacaaaaacc ggcaagcgtg tgaccgacaa
     4501 ggacggcgcg acgatcaagc tggtgctgcc gcatggcgaa gagatacccg ggcgatgggc
     4561 aattcgcggc ggggcggcag cgatgccgga ttccgcctgg atgtgggata ccccctggga
     4621 catcccgcct gattatccga tcggctcatt cgattatcgg atcacgattg ctgcaaggga
     4681 tgggcgcacg gccatttcac tccgccaatc cagaaggcga ccaccgccga cagccgcgtt
     4741 cggattatcg actagcgtca gtatcgtgct tcgcggcttc aaaattgtcc aacttttggt
     4801 gctgcttgcg gcgtgcgggg gatggatcgc gctttatgca ggcggcggtc ctcccgctat
     4861 cgcagtagca gactcgtccc ggactgctca gaacgaaaga ttccaatccg ctgcacaagc
     4921 gcctgtaggt caagagcttg cgccggcgct gaagcttctc aaagtcgaac cggtgaaagg
     4981 ttatgttagt gatccgttca ctgtgagcgg cgaaggcttt gccgctgggg ctgcggttga
     5041 atttttttgg agcacggttg agccgagtta cctcaccaaa gtcttacccg ataacctcga
     5101 ataccacgag cgcaagtacg acgagaaacg tgtcgtgctg ggccttgccc aggtggatgc
     5161 ccaggggcgg gtcaatgcga aattcactgc cccagaagac ttcggcgaag tgcatgatgt
     5221 attcgccgtt gtcgaaggtc gcgacctggc gcgcggcggc tttcgcattt tacgcagtgc
     5281 cagcttaact cccaagaccg gatccgtggg cacaccgatt actgtcaccg tgaatgggat
     5341 cgggtggcat ggtttcgagc aatttatggc gctccggtat gacaacaaat ataccggtga
     5401 aatctctgcc gtcacgacta aaggcagcgc ggtgtttcag attcgagccg ccggcgagcc
     5461 gggtaaacat atcgtccaac tcaacaacag caccgcctat tcgccaggcg cctaccttaa
     5521 cgcgcagcaa tcgccgcagg cctacattta tgcgcatctc gataatcagc aggagtttcg
     5581 gttcaccttc gatatcacga ccgacaacga aattcctccc gacaggatcg aatggccgga
     5641 gcaggggcgc gttgccgtgc tcagtgccga tgcgccgcgc accacaatga gcaacaaatc
     5701 ggtagtggga gcggcgacgt tgacgcctgc ttcggggaca gtaaataccc aagccactgt
     5761 cagcgcaaag aacctaccga gcaatacgca ggtcgagctt cagttcatta cagcgcgcgg
     5821 caaccgtatg actccaagcg gatggaacct cgattatctg cccgttggca aggctagaac
     5881 ggcaggcgat ggatcactga aacaaaccgt cacgatgccg gacgacctcg gcggctggca
     5941 cataattagg attgccacgg aggaccgtgt gctcgccgag acaccttatt atctcgaaca
     6001 gagcttggtg caagtttcgc cgcggcgggt gaaggtcggc gaagtcttca cagtacaaat
     6061 caagggcgtt ggctggaccg agctcgataa cacgttggct gtgacttatg acaacgctgt
     6121 cctgggatat gcttgcggtt ttaacaccaa cggtgatgtg acggtgaaca tggtgggcac
     6181 cggccgcccg ggaattcatc tggtcgattt ctatccggcg atctatcgcg gcagagaccg
     6241 agtgccgtgg aattaccaga cgccatttct tacttacgcc cgcgacttcc cggcattgcc
     6301 gcacggctac cgattgccgg cttatcggct cgcaatcgag gtgatcgaat agttgccgtt
     6361 ttttagtcgc gctcaatttc gccggacaat cacgcagctt caatctcggc gagttccagg
     6421 cgcacagcgc tttcctgcgg atctcaactc acctcgatcg tgagcatgag cgagttgaga
     6481 acgagctttt cctgcatgcc gatgaaggcg tagtcgttga actggtatga accgaatggc
     6541 tcgctagtgc tggctggtgt agctgtttgg ttgtcaggcg caggccgtca cagtgcgtca
     6601 cgaaatcaac caacggagta ctacagtgac gctttaggaa agctcagcgc atcgatttcg
     6661 aactttctca gttctggctg cacttgggcg gtgaaaagct tgagcgaact aagcacctgt
     6721 ttctgagtga gctcgccaaa agtaaattcg cccaagaaag tgtcagcgcc ggcttggctg
     6781 gcgattttct tgatttgatc ggcaacggtt tcgggggaac cgcagatgaa tgagccttcg
     6841 tcaatcgagc gctccatgtt catcagttcg tgacggcggc tgccatcaag aatggtcgat
     6901 aatcc
//
LOCUS       DASULZ010000664         2065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_176626, whole genome shotgun sequence.
ACCESSION   DASULZ010000664 DASULZ010000000
VERSION     DASULZ010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176626"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VFY96_13795"
     CDS             complement(<1..605)
                     /locus_tag="VFY96_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607591.1"
                     /translation="MLNSKDRQESHLRNLKQLTFGGQNAEAYFSSDATKIIFQSTRPP
                     YQCDQIFSMNIDGSDVKVLNSGKGRTTCGFYFPNGKRIIYASTHLTSPICPPQPDRSQ
                     GYVWPVYPSYEIFSANSDGSGMKRLTKNPGYDAEATISPDGKKIVFTSMRGGDLDIYT
                     MNADGSGVKQLTHEKGYDGGPFFSWDGKTIVYRAYHPKSKK"
     gene            1103..>2065
                     /locus_tag="VFY96_13800"
     CDS             1103..>2065
                     /locus_tag="VFY96_13800"
                     /inference="COORDINATES: protein motif:HMM:NF020896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HEX5607592.1"
                     /translation="MKLDVADFPVHEIRFGNSYRYEKGKLTVDREDLARLVSEDARIA
                     QARFEAVEPGEHARITGIRDVVEPRVKFSARGQVFPGVMGSVESVGAGMTHRLSGMTV
                     IATAYYEGTIRAGTGVQRSALLDMWGPGAEASRFSHYPHLVLIVRLKEGLGEFEAHLA
                     IQRAEFAVARRIAQVTEGAKPEEVERFELNEDESLPRVLLIQGCITNGGQPHSGVSYY
                     GLPIRESLATWIHPNELLDGAMTTNTTRGIGYYPTTWDWQNHPLALELYRHQAAGRLS
                     FAGMIVERISYDTIHGKEVVAHNTAQLAAHAKADAALITWLGSGN"
BASE COUNT          479 a          523 c          593 g          470 t
ORIGIN      
        1 tcttttttgc ttttcggatg gtacgcacga tagacgatgg ttttgccgtc ccatgaaaaa
       61 aacggcccgc cgtcgtaacc tttctcatga gtcaattgtt ttacaccgct accgtcggca
      121 ttcatcgtat aaatatcgag atcgccgccg cgcatagagg taaagacaat ttttttgcca
      181 tcgggtgaaa ttgttgcctc ggcatcgtaa ccggggtttt tcgtcaagcg cttcatcccc
      241 gaaccgtcgc tattggcact gaagatttcg taactgggat aaactggcca gacgtaaccc
      301 tggctgcgat ccggctgcgg cggacatatt ggactggtta aatgtgtaga ggcatagata
      361 attctcttgc cattgggaaa gtaaaagccg caggtcgtgc ggcccttgcc gctgttcaaa
      421 accttcacgt cgctaccgtc gatattcatg ctaaagatct gatcgcactg gtatggcggg
      481 cgagtggact ggaaaatgat cttagtagca tcagacgaaa agtaggcctc ggcattctgg
      541 ccgccaaacg tgagctgctt taagttgcgt aggtgagact cctgacgatc cttactattt
      601 aacatcggga ctggctcttg cgatctaagc ggcatcaagg caacgatcat tagcaagccc
      661 gtcagaacaa cgcacagaag aaattgtttc ataccgaggc gactatatta tgaacgtttc
      721 gctgccagac tttcttcgac tgtaattcaa gaaaccgccg atgtcatcaa ctcaacggat
      781 gtcgcgaaga cctctcgacc gtggcagtca tttcgataag agcggcgccg gcggttgcac
      841 gtccggaggc tctgtggttt aacgcggctt accgctcacg aagattcgca atcggcggcc
      901 cacaccaccg gtaactctcg acgctcacga cctggtaaac atgaagcttg tgcgcgccta
      961 tcgcacttgc gggtatctgg aagctgcgac gcaagacttg gttgatgacc gttaagaaac
     1021 tggacaaaga aggattgttt tgattgaaaa tgcacgagga agacgtccca agcggctctt
     1081 ttgggataat acaggccaaa ccatgaaact cgatgtggcg gattttccag tgcacgaaat
     1141 tcgtttcggc aactcttatc gatacgaaaa aggtaaattg acggtcgatc gggaggatct
     1201 ggcccgcctc gtgtcggaag acgcacggat tgcgcaggcg agattcgaag ccgtcgagcc
     1261 aggcgaacac gcgcggatca ccggcatacg cgacgtcgtc gagccgcgtg tgaaattcag
     1321 cgctcgaggt caagtttttc cgggagtcat gggctcggtg gaaagtgtcg gtgctggcat
     1381 gacgcaccgg ctgtccggga tgacagtgat cgccacggca tattacgaag gaaccattcg
     1441 cgcaggcacg ggtgttcagc gcagcgcgct gctcgatatg tggggtcccg gggccgaagc
     1501 atcccggttc agccattatc cgcatttggt tttgatcgtg cgcctcaaag aaggtttggg
     1561 tgaattcgaa gcgcatctgg cgattcaaag agcggaattc gcggtggcga gacgaatcgc
     1621 ccaggtcacg gagggcgcaa aaccggagga ggtggagcgg ttcgagctga acgaagatga
     1681 atctctgccc cgggttcttt tgatccaggg ctgcatcacc aacggtggac agccgcactc
     1741 cggggtcagc tattatggtc tgccgattcg tgagtcgctg gcgacttgga tccatcctaa
     1801 cgagttgctc gacggcgcga tgacgacgaa cacgacacgc ggcatcggtt attatccgac
     1861 gacgtgggat tggcagaacc atccgctcgc tttggaactt tatcgtcacc aggcggcggg
     1921 ccggctgagt ttcgccggca tgatcgtgga gagaatttcc tacgatacga ttcacggcaa
     1981 agaagtggtg gcgcacaata ccgctcagct tgcggctcat gcgaaggcag atgctgcgtt
     2041 gatcacttgg cttggctctg gaaac
//
LOCUS       DASULZ010000665         2961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1395597, whole genome shotgun sequence.
ACCESSION   DASULZ010000665 DASULZ010000000
VERSION     DASULZ010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395597"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1297)
                     /locus_tag="VFY96_13805"
     CDS             complement(149..1297)
                     /locus_tag="VFY96_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HEX5607593.1"
                     /translation="MESTLVAGLQGLFLGVLNLSIGHVIMLAIACLLLYLGIGKGYEP
                     LLLVPIGFGAILVNIPLADMMGKDGFLRFFYDAGVLTEVFPLLIFIGIGAMTDFQPLL
                     QNPKIILLGAAGQFGIFLTLLLALALGMERLDAVAVAIIGACDGPTAIYVSSKFAPNM
                     LGAVSVAAYSYMSLVPLIQPPIMRALTTEKERQIVMGAAKLQPISRATKVAFPIIVTI
                     FASILAPKGAPLIGMVMLGNLMRESGCVERLKSAAENEITNIVTLLLGLCIGATMAAD
                     NFLRSQTLLVLALGFIAISLDTAVGVLFGKAMSFLTGGKINPLIGAAGISAFPMSARV
                     VQTEGQKYNRKSYLLMHAMSANAGGQIGSVIAAAVMLSVLQGMGIVGG"
     gene            complement(1307..1453)
                     /locus_tag="VFY96_13810"
     CDS             complement(1307..1453)
                     /locus_tag="VFY96_13810"
                     /inference="COORDINATES: protein motif:HMM:NF040909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG-related small transporter subunit"
                     /protein_id="HEX5607594.1"
                     /translation="MDNWTFGWTLALIGAIGTMLVLWIMSLLILLIRKIFPYVPEDSA
                     AKKG"
     gene            complement(1531..1746)
                     /locus_tag="VFY96_13815"
     CDS             complement(1531..1746)
                     /locus_tag="VFY96_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEX5607595.1"
                     /translation="MATTVEAPMVGKILKIEKQVGEHVDEDEVVIIMEAMKMEIPIVA
                     PASGTVKEIKVSPGEAVEAEQALAVID"
     gene            1973..2932
                     /locus_tag="VFY96_13820"
     CDS             1973..2932
                     /locus_tag="VFY96_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia lyase-activating protein"
                     /protein_id="HEX5607596.1"
                     /translation="MSKPAEQFDNVMDNIPYFDKWQMGEGIPIIKTFFVQDLKKVELK
                     PWDRTGGYGAFINMEGAEGATGAYISEIPAGKKLNPMRHLYEDLHFVLKGRGGTTIWN
                     DKGAKQNFEWQEGSLFALPLNCWYQHFNGSGSEAARFFSVNSMPIVFNLFHSADFVFS
                     TPRDFTDRFDGDPEYFSGKGKAYPGRVWDTNFIADARNFALEDWKERGAAGKNVMLEL
                     ANGSMAAHISEFPIGTYKKAHRHGPGFNVIIIKGKGFSLFWREGEEPKRYDWQDGSVF
                     TPPAMMFHQHFNTGATGARYLPPRLGGIKYSLGEGFGDITKVD"
BASE COUNT          700 a          782 c          808 g          671 t
ORIGIN      
        1 agagacggtc gatgcgctcg cgcgcggtga gctttccttc ggcacgttgc tttgcggcag
       61 ttttcggatc ggcatcgacg gcgatttttt cgagtgccgc gagttttttc tccagatctt
      121 ccctggtttg cgccatcgat cgcctcgttt agccgccgac aatacccatg ccttgaagta
      181 cggagagcat taccgccgcc gcgatgacgg agccgatttg tccgccggca ttggcgctca
      241 tggcatgcat caaaagatag cttttgcgat tatatttctg cccttcggtt tgcacgactc
      301 gggccgacat cggaaaagcc gaaatgccgg cggcgccgat caacggattg attttgccgc
      361 cggtcagaaa ggacatcgct ttcccaaaga gcacgccaac ggcggtgtcg aggctgatcg
      421 ctatgaagcc cagggctaaa accagcagtg tctgagaccg taaaaagtta tcggcggcca
      481 tggtcgcgcc gatgcagaga cccagcaaca aagtcacgat attggtaatc tcgttttccg
      541 cggcgctctt tagacgctcc acgcaaccgc tctcgcgcat caagttgccg agcatgacca
      601 tgccgatcaa tggcgcgcct ttgggcgcca gaatggacgc gaagatcgtc acaataatcg
      661 ggaatgcaac ttttgtggcg cgagaaatcg gttgcaattt tgccgcgccc atgacgatct
      721 ggcgctcttt ttccgtggtg agcgcacgca tgatcggcgg ctggatcagc ggcaccagcg
      781 acatgtatga ataggcggcc actgagaccg cgccaagcat attgggcgcg aatttcgagc
      841 tgacgtagat cgcagtaggt ccgtcgcagg cgccgatgat cgcaacggcc acggcatcga
      901 gcctttccat gccgagggcg agcgctagca acaaggtcag aaaaatcccg aactgtccgg
      961 cggcgccgag caaaatgatt ttcgggttct gcaggagcgg ttgaaagtcg gtcatcgcgc
     1021 cgatgccgat aaaaatcagc agcggaaaga cttccgtgag cacgcccgca tcataaaaaa
     1081 atcgtagaaa cccgtctttg cccatcatat cggcaagcgg gatgttgacc agaatagcgc
     1141 cgaatccaat cggcaccaga aggaggggtt cgtatccctt tccgatcccc aggtacagca
     1201 gcaggcatgc aatcgccagc atgatcacat ggccgatgct gagatttaac acaccaagaa
     1261 aaagcccttg caggccggca acgagcgtcg attccatacc tgagggctaa cctttcttgg
     1321 ctgcggaatc ttccgggacg tagggaaaaa ttttcctgat cagcaaaatg agcagactca
     1381 tgatccagag caccagcatc gttccgatcg cgccgatcag cgccagtgtc caaccaaatg
     1441 tccaattgtc catatcaata agtcttcgag gcgtgagtct tagagcagcc gaggcggggc
     1501 tacccgagtt cattgatcaa tgttttcaca tcaatctata acggcgaggg cttgctccgc
     1561 ttccacagct tctcccgggg agactttgat ttcttttacc gtcccgctcg ccggcgcaac
     1621 aatcggaatc tccatcttca tcgcttccat tatgattacg acttcgtctt cgtcgacgtg
     1681 ttcgccgacc tgtttttcaa tttttaaaat ttttcccacc atgggcgctt cgacggtcgt
     1741 tgccaacgta ttctcctttt agggacatag ccgccggcgt gagacttatg caggcggcgc
     1801 acagcgttaa cgtgccattt agtaaagttt actctgggtt gtcaatgcta cgagagagaa
     1861 ttgccgattg ttgcggttga tacgatgcgg cagcggcgtt ccgcgtgtca gatcaccttg
     1921 cttttgctca gcctatgcga tactaaaaat gtagttttcg gagggaaaaa ttatgtctaa
     1981 accggcagag caattcgaca acgtcatgga caatattccg tactttgaca agtggcaaat
     2041 gggcgaggga attcccatca tcaagacttt ctttgttcaa gacttaaaaa aagtggagct
     2101 gaaaccatgg gaccgcaccg gtggttacgg tgcgttcatc aacatggaag gagccgaagg
     2161 cgcgaccggc gcctatatca gtgagattcc tgccggaaaa aaactcaatc cgatgagaca
     2221 tctgtatgaa gatttgcatt ttgttctcaa gggacggggc ggcacgacga tctggaacga
     2281 caagggcgcc aaacaaaatt tcgaatggca ggaagggagc ctgttcgcgc tgccgctcaa
     2341 ttgctggtat cagcacttca acggctcggg cagtgaggcg gcgagatttt tttctgtcaa
     2401 cagcatgccg atcgttttca atttgtttca tagtgctgat tttgttttca gcacgccgcg
     2461 cgacttcacg gatcgcttcg atggtgaccc ggaatatttc agcggcaaag gcaaggccta
     2521 tcctggccgg gtttgggaca ccaatttcat cgccgacgcg cgcaatttcg ctcttgaaga
     2581 ttggaaggag cgcggtgccg cggggaaaaa cgtaatgctc gagttggcga acggctcgat
     2641 ggccgcgcat atctcggaat ttccgatagg cacctataag aaagcgcatc gccatggacc
     2701 gggcttcaat gtgattatca tcaaaggcaa aggattttcc ctgttctgga gagaaggcga
     2761 ggagccaaag cgttatgact ggcaagacgg cagcgtcttt accccgccgg caatgatgtt
     2821 ccaccagcac ttcaatacgg gcgcgacggg cgctcgttat cttccaccgc gactgggcgg
     2881 tatcaagtac agtcttggcg agggattcgg cgacatcacc aaagtcgatt aggacgtcaa
     2941 agcgggcggc aatcaaatcg a
//
LOCUS       DASULZ010000666         2894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1293322, whole genome shotgun sequence.
ACCESSION   DASULZ010000666 DASULZ010000000
VERSION     DASULZ010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293322"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VFY96_13825"
     CDS             complement(<1..418)
                     /locus_tag="VFY96_13825"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX5607597.1"
                     /translation="MKALALAVLGLFFIVAARPRHLRRLQIIGLAGGWLALTGALATP
                     LDELAETSLSAHMLQHEILILIAAPLLALGRAHHLLLALLPRSRRGGLWQYVRSIRVS
                     TALACILHALAIWIWHLPACYEAAATQPFLHLAQHAS"
     gene            complement(434..772)
                     /locus_tag="VFY96_13830"
     CDS             complement(434..772)
                     /locus_tag="VFY96_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607598.1"
                     /translation="MSLFALWTGMVGGPLIWLIVLEFNYAWAPAACRSGDKIGLVIGT
                     TVALVLSLIAAFIAWRSWHAAGPVVTTAAGDPLTRGRFMALTGLGVSALMTLLIIASF
                     LPIAFLEVCD"
     gene            complement(769..1374)
                     /locus_tag="VFY96_13835"
     CDS             complement(769..1374)
                     /locus_tag="VFY96_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX5607599.1"
                     /translation="MNPDLTIDVRTLPAKAFGPRTTIWWGVLGLLTIEGTMFGILVAS
                     YLYLTKNFSAWPPLGTPPPDLGAATANMILLLASVWPMRVAHRAAFIEQRRPIALALA
                     VCTLAGLGALVLRFFEFRAMHIRWDTNAYGSIVWTILGMHTGHLIASTLENILLMALM
                     RAGPVEAKHFVDTNVNAVYWYFVVFAWLPIYCLVYFAPRAL"
     gene            complement(1371..>2894)
                     /locus_tag="VFY96_13840"
     CDS             complement(1371..>2894)
                     /locus_tag="VFY96_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012760472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX5607600.1"
                     /translation="WVSFFLNIGPDTGWFSYVPLSGPEYSPGKRVDVWAQMITFTELG
                     SLVGAVEIIVTIFKLRAPGMSLNRMPIFVWAQLVTSFMVIFAMPAVMLASGFLAMDRL
                     VDTHFFNPAEGGDALLWQHLFWFFGHPEVYIIFIPATGMVSMLVQAFARRPMFGYTAV
                     VLSLIATGFLGFGLWVHHMFAVGLPQLGESFFTAASMLIAIPTGVQIFCWIATLWKGR
                     VLIRTPLLFIAGFIFTFVLGGLTGVMQAAVPLDLQLHDSFFVVAHFHYVLIGGAVFPL
                     IGALYFWFPKMTGRMMDEKLGQVNFWLFFIGFHLTFFPMHQLGLNGMPRRVYTYLPET
                     GWGRLNLIATCGAIVLGLAVLLFLVNIYVSRKSGVLAGTNPWGADSLEWDIPSPPPNY
                     NYLYLPTVNSRTPLWTKTPDQPVVTGMRSDILEVLVTTLHDALPDHRHLLDGPSYWPL
                     LTALAVGIGIITAIFTPWGITIGAVLSFLALAGWFWPLGEPIADTSREAGPPFREAA"
BASE COUNT          732 a          836 c          861 g          465 t
ORIGIN      
        1 agctcgcgtg ctgcgcgagg tgtaaaaatg gttgggttgc tgcggcttca tagcacgccg
       61 gcaagtgcca gatccagatc gcgagcgcat ggaggatgca cgcgagtgcc gtgctcacac
      121 ggatgctgcg gacgtactgc cagagcccgc cgcgacgaga gcgcggcagg agcgcgagca
      181 aaagatgatg tgccctgccg agcgctagca ggggcgcggc gatcaggatc agaatctcat
      241 gttgcagcat gtgagccgac aaactggttt cggcgagctc atcgagcgga gtggctagag
      301 cgccggtgag tgctagccat ccacccgcga ggccgatgat ctgcaggcgg cggagatgtc
      361 gaggccgcgc ggccacaata aagaagagcc caagaactgc gagcgccaac gctttcatga
      421 gcatttccat cgctcagtca cagacctcga gaaatgctat cggtaggaaa ctcgcgatga
      481 tcagaagagt catcagcgcg gacacgccaa gccccgtcag tgccatgaag cgacctcgcg
      541 tcagggggtc tcctgctgcc gtggtcacga ccggtccagc agcatgccac gagcgccatg
      601 caatgaacgc cgcgatcaag ctgagcacga gcgcgaccgt cgtgccgatc actaggccga
      661 ttttatcgcc ggaccggcat gcggctggcg cccaggcata attaaattct agaacaatga
      721 gccagataag cgggccaccg accattccag tccagagcgc aaacagactc atagagcacg
      781 cggcgcaaaa taaaccagac agtagattgg cagccaggcg aatacgacga agtaccagta
      841 caccgcgttc acgttggtat cgacgaagtg cttcgcttcg acagggccgg cgcgcatcag
      901 cgccatcagc agaatgtttt ccagggtcga ggcaatgaga tgccccgtgt gcatgcccaa
      961 tatcgtccag acaatcgagc cgtaggcgtt cgtatcccag cgaatatgca tggcgcggaa
     1021 ctcgaaaaat cgaagcacga gcgcgcccaa tcccgccagc gtgcaaaccg ctagagcaag
     1081 cgcaatcggc cggcgctgct cgataaaagc ggcgcgatgc gccacccgca tgggccagac
     1141 gcttgcgagc aacagaatca tgttagcggt tgcggcgccg agatcgggcg gtggtgtgcc
     1201 gaggggcggc caggcggaaa aatttttcgt caaatagaga tagcttgcga ccagaatccc
     1261 gaacatcgtg ccttcgatcg tgagcagccc cagcactccc caccaaatcg ttgtgcgcgg
     1321 accaaaagcc ttggccggca aagtcctgac gtcgatcgtc agatcggggt tcatgccgcc
     1381 tccctgaatg gcggcccggc ttcacgcgag gtgtcggcga tcggttcgcc gagcggccag
     1441 aaccaacctg cgagcgcgag gaagctcaag accgcgccaa tcgtgatccc ccagggtgtg
     1501 aagatggcgg tgataatgcc aatgcccacg gcgagcgcgg tcagaagcgg ccaataactc
     1561 ggcccgtcaa gaagatgacg atggtcgggc agtgcatcgt gcagcgttgt caccaacact
     1621 tcgaggatat ccgagcgcat accggtaacg acaggctgat ccggcgtttt ggtccagagc
     1681 ggtgtgcgac tgttgactgt cggtaaataa agataattgt agttgggcgg cggtgatgga
     1741 atgtcccact cgagtgagtc ggccccccac ggattcgttc ccgcaagaac gccgctcttt
     1801 cggctcacat aaatattgac gagaaacagc agcaccgcga gtccgagcac aatcgcgccg
     1861 caggtcgcaa tcagattcag ccggccccac ccggtctcgg gtagataggt atatacacgg
     1921 cgcggcatgc cgtttagtcc caactgatgc atgggaaaaa aagttagatg aaaaccgata
     1981 aagaaaagcc aaaaattcac ctggccaagc ttttcatcca tcatgcgccc ggtcatcttt
     2041 ggaaaccaga aatagagcgc gccgatcaac ggaaaaacgg cgccgccgat cagcacataa
     2101 tgaaaatgcg ccaccacgaa gaagctatcg tgcagctgaa gatcgagtgg caccgcggct
     2161 tgcatcactc cggtcagacc gccgaggaca aaagtaaaaa taaaaccggc gataaacagc
     2221 agcggcgtgc gaatgagcac gcggcctttc cagagggttg cgatccagca aaagatctgc
     2281 acaccggtgg ggattgcgat cagcatgctc gccgccgtga agaagctttc gccgagctgg
     2341 ggcagcccca cggcaaacat atggtgcacc cagagtccga aaccgagaaa gccggttgca
     2401 atcagtgaca gcaccaccgc cgtgtagccg aacatcggcc ggcgcgcgaa cgcttgaacc
     2461 agcatcgaaa ccatgccggt ggccggaatg aagatgatat aaacctcggg atggccgaaa
     2521 aaccaaaaca gatgttgcca cagtagcgca tccccgcctt ccgccggatt gaaaaagtgc
     2581 gtgtcgacca gccgatccat ggcgagaaag ccgctggcaa gcatgactgc gggcatcgcg
     2641 aagatcacca tgaacgacgt cacgagctgg gcccagacaa agatcggcat gcggttgagc
     2701 gacatgccgg gagcgcgcag cttgaatatc gtgacgatga tttcgactgc accgaccagg
     2761 gatccgagtt ccgtaaacgt gatcatctgc gcccagacgt cgacgcgctt tccgggagag
     2821 tactccggcc cggaaagcgg cacataactg aaccagccag tatccgggcc tatattcaga
     2881 aaaaagctga ccca
//
LOCUS       DASULZ010000667         2323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1089168, whole genome shotgun sequence.
ACCESSION   DASULZ010000667 DASULZ010000000
VERSION     DASULZ010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089168"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VFY96_13845"
     CDS             complement(<1..606)
                     /locus_tag="VFY96_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607601.1"
                     /translation="MLYTQNSQSPTPHILSSGLLTLVLLFAGCRLMADYDDYVIADYG
                     HLAVRSESQMNGFVLGVPHGTTDPDAVDYAKSVSDATGAGIVIAYGMKNKRITVAQPV
                     IPNSAVSWNNASATRPGSIYSDFKNSLQSTTVGPIRFYVEFRTARAKLERPRIEVASA
                     GFSFEQLQELKRFYNAIETRSLQNRGMAPVELVINPLDRISW"
     gene            1542..>2323
                     /locus_tag="VFY96_13850"
     CDS             1542..>2323
                     /locus_tag="VFY96_13850"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607602.1"
                     /translation="MNRSIIGIGVLAVVFWAFLGAGRATASESVHLAYISDSPGSSAP
                     YWIAKEAGFYKKHGLDVELIFINGSTRGIQSLIAGDLDFAGAVGTSAINGKLAGGDIY
                     IVDSLVNTLPYYIIGKPTIKSPEDLKGRTLATHIPGTSADFAVRLALRKFGIDYQDIR
                     AVMVGGSTARVAAVINGQTDFTMVTEPGKLQGENAGMNVIIDMAKLKIPFQFSCTVAS
                     GKMIRENPKLVETMVKSMAEAVHFFKTQKKESIRIMAKYTRGA"
BASE COUNT          559 a          596 c          634 g          534 t
ORIGIN      
        1 ccaggaaatc cggtcgaggg ggttgatgac aagctcgacg ggcgccattc ctcgattttg
       61 taaggaacga gtttcgatcg cgttatagaa gcgcttcaac tcctggagct gctcgaaact
      121 gaagccagcg gatgccactt caatgcgcgg ccgttcaagc ttggccctgg cagttcgaaa
      181 ttcgacatag aatcttattg ggccaacggt ggtcgattgc agcgaatttt tgaaatccga
      241 atagatgctt cccggacgcg tcgcagacgc gttattccaa gacacagcac tgttgggaat
      301 taccggctgg gccacggtga tgcgcttgtt cttcatgcca taagcgataa cgatcccggc
      361 gccagtcgcg tcgctaactg atttggcata gtcgacggca tccggatcgg ttgtgccgtg
      421 gggcacgccg aggacaaagc cgttcatttg cgattcggaa cgaactgcaa gatgcccgta
      481 atcagcgatg acataatcat cgtaatccgc catgagcctg caaccggcga acaagagcac
      541 caaggtcagc aaacccgacg atagaatatg cggcgttggc gattggctat tctgagtgta
      601 cagcattcga ggaatctggc gagtcagaat aaggtgacgg tgctggcaag aagccgcgcc
      661 ggtgagagga attacttgta tccaacttct tctatggtta tgcgcagtcc gaaattgctg
      721 ccgtccgacg agcaatgtgc atgccggctt ttaatcgcga ttgtcagcct aacgcgcaaa
      781 ggtgttcttg cttctggcac ggctcgtaga gttagttaga catgctgctc aggcaaaagc
      841 tgccgtggag atggatgaga tacagcgcaa acagctgggc aaagccgttc gttacggctg
      901 gatcggaggg cttctgtgaa gatcttgcac atgctctggc gatgcacatc ggctaaaaag
      961 aaaaaaaggc tttcgtttaa ggggagagca gccctctagt tcgaagcggc actgtgaaca
     1021 tcggtaacac tgatctcgtg tcagcgcttt gggctgtgaa attcctgtac cctcggcgcg
     1081 atatctcgtg ctgcgcacgg gattagcgca tcgatttacc tggcattttc cgaccacgat
     1141 cactggcttt cttcttgctg gttgaacaaa ccagcgcgaa aggtgttgat ctgaagacta
     1201 gctcaaattc gacttgtgca ttgcccgggg gaagacgatc ggagattgga atcggttctc
     1261 agcagtctgc gcgtccagtt tacttccgag tacatgaccc gatctcatcc gtcggtcttc
     1321 ccgtttgccc aaaacaaatc gatgtccgcc cgtcatcgcg cggcgcgccg ccgctaaaaa
     1381 tttagcactg ccgcaaaatg agcgagggac cattgagcca aacaacaatc gacagccctc
     1441 gctatcaaca gcaagcaaga tcctcgttgg gttcttcgcc agacgaagaa tttcttctgt
     1501 tctccaacag attttccgaa tcaaaaactt tcggaggagt tatgaatcga tcaataatag
     1561 ggatcggagt actcgccgtc gttttttggg cgtttctcgg tgcgggtcgc gccactgcga
     1621 gtgaaagcgt tcatctcgcc tatatctcgg attcccctgg ttcatctgcc ccgtattgga
     1681 ttgccaaaga ggcggggttt tacaagaaac acggattgga tgtcgagctg atctttatta
     1741 acggtagcac gcgcgggatt caaagcttga tcgcggggga tctggacttt gccggtgctg
     1801 tcggtacttc ggcgatcaac ggtaaactgg ccggtggcga tatttacatt gtcgacagct
     1861 tggtaaacac gctgccttac tacatcatcg gcaaaccaac gatcaagtcg cctgaggatc
     1921 tcaaaggccg cactttagca actcacattc ccgggacttc ggcggacttc gctgtgcgtc
     1981 ttgccctccg taagttcggc attgactacc aagacatccg agcggtcatg gtcggcggct
     2041 caacggcacg ggtcgcggcg gtgatcaacg gccaaaccga tttcacaatg gttacggagc
     2101 cgggaaaact tcagggcgaa aacgccggca tgaacgtgat catcgacatg gccaagctta
     2161 aaattccatt tcaattcagc tgcacggtgg cttcagggaa gatgatccgg gaaaatccca
     2221 agctcgtcga gaccatggtc aagtccatgg ccgaagcggt gcatttcttc aaaacccaaa
     2281 agaaagaatc aatccgcatc atggcgaaat acacgcgagg cgc
//
LOCUS       DASULZ010000668         5086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_989389, whole genome shotgun sequence.
ACCESSION   DASULZ010000668 DASULZ010000000
VERSION     DASULZ010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989389"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..924
                     /gene="gnd"
                     /locus_tag="VFY96_13855"
     CDS             28..924
                     /gene="gnd"
                     /locus_tag="VFY96_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEX5607603.1"
                     /translation="MELGFIGLGRMGANMTERLVLGGHRIISFDRNPEAIQRVVDKGA
                     LGARSLADFVQQLSLARAIWLMVPSGDPVDQTIEQLLPHLSEGDMLIDGGNSKYKDSI
                     RRAEKLKPHGIHFVDAGTSGGIWGLQNGYCLMVGGEKAVVDHLEPILKTLAPPDGYLH
                     VGPVGAGHFVKMIHNGIEYGMLQAYGEGFELLKASQFKLDLGKISHLWNQGSVVRSWL
                     LQLAEDAFKEDPELASIRGYVEDSGEGRWTVEEAIEKGVPTPVLMLSLFARFASRQED
                     SFSAKVIAALRNEFGGHAVKRG"
     gene            929..2482
                     /gene="zwf"
                     /locus_tag="VFY96_13860"
     CDS             929..2482
                     /gene="zwf"
                     /locus_tag="VFY96_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017739780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX5607604.1"
                     /translation="MAPDPHDYVLNTGDGAGPAKKPHACVLVIFGASGDLTKRKLIPA
                     LYNLALENRLPERFAVVGYARSEMSDDVFREKMRDAVREFSRTGLPNESVWAQFAATL
                     YYVRGGYEQNDGFRRLKELVEGFDRGSRVLPARVFYLATPPDAYEQLIQCIARTGLAS
                     TESDGEQRARVVIEKPFGTDLQTARELNQRIHEVLDERQIYRIDHYLGKETVQNIMVF
                     RFANAVFEPIWNRRYVDHVQITAAESVGVENRGGYYEQAGVVRDMFQNHLLQLLCMTA
                     MEPPVGLDADSVRDEKGKLLKSVRPVAAEEVAAAAVRGQYGPGRVDGQEVPGYRQEPG
                     VAKNSSNVTYTAIRFFIDNWRWEGVPFYLRSGKRMPKRVSEVAIQFKRPPMLLFKSHA
                     VEDVNPNVLVMRIQPDEGVSLTFEVKPPGHDMIIRPLCLDFDYEESFGNSPPEAYETL
                     LEDCIEGDSTLFTRHDWVELAWSLVDPIIQVWNLSRPRNFPNYEAGSWGPKEADEFMA
                     REGRRWRKP"
     gene            2810..3805
                     /gene="glk"
                     /locus_tag="VFY96_13865"
     CDS             2810..3805
                     /gene="glk"
                     /locus_tag="VFY96_13865"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEX5607605.1"
                     /translation="MSHKLVLAGDIGGTKTSLALFSVHGDKLRIEAERNLPSKQYSGL
                     TPILEEFGVHKQVIDSACFGIAGPVVDGRVKTTNLPWIIDSQVLSQELKVETVYLLND
                     LEATAYGVFTLEDDEFYPLNQGILHHTGNKALIAAGTGLGQAILYDDGRHFRALPSEG
                     GHADFAPRDELEIELLRYLFARFGHVSYERVLSGPGLFNIYRFLKESRGMEEPLWLAE
                     HFAAGDDPGATISNAALAGKAEICVKALDLFVSIYGAEAGNMALRFKAVRGLYVGGGI
                     APKILEKLKDGTFMRSFIDKGRYTELLTAMPVQVVLNPQAGLRGAGYYAAFLSGS"
     gene            complement(3973..4563)
                     /locus_tag="VFY96_13870"
     CDS             complement(3973..4563)
                     /locus_tag="VFY96_13870"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(4324..4326),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HEX5607606.1"
                     /translation="MRTQFFLAAALAFCLGSTTVLAELGPKDGSDLAPTDLNRIKVGQ
                     PAPDFSLENVDGNMITLSDFRGKKTVVLVFYRGYWUPFCVEQLGKLKSLLTKQQKEKT
                     QILAVSVDTHAESKKFGEMLSKRFEGEFDFPLLEDKDHKVINRYGVFNPDGRGWPHPS
                     TFLIDPQGVVRWKDIETDYKKRPSNEQIRRELAKIK"
     gene            complement(4584..>5086)
                     /locus_tag="VFY96_13875"
     CDS             complement(4584..>5086)
                     /locus_tag="VFY96_13875"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5607607.1"
                     /translation="YLGAVYCTHEALPSMIARRIGHIVNISSGAGKIGTLNLAAYCGS
                     KFALNGWSESLYHELKPLGIKVSVICPGPVQTDFNRDFRDSEPKAPPAMVVSTEAVCR
                     EVIEAIDKDKFEVVVPRSLALLSAFARVMPGLFRALAQRRFRNYVKVPASTVDPLTMT
                     GTGGVD"
BASE COUNT         1108 a         1406 c         1444 g         1128 t
ORIGIN      
        1 gattaggtct tcccaggagg aacgctcatg gaacttggat tcatcggcct tggacgaatg
       61 ggcgccaata tgaccgagcg actcgtcctc ggtggccatc ggatcattag cttcgatcga
      121 aaccccgagg cgatccaaag agttgtcgac aaaggcgcgc tgggcgcccg ctcgctggct
      181 gattttgtcc agcagctgtc gctagcacgg gcgatttggc tgatggtgcc ctcgggagat
      241 cccgtcgatc agacgattga acaattgtta ccgcatcttt cagaagggga catgctgatt
      301 gacggcggca attccaaata caaagactcc atccggagag cggaaaaact gaagcctcat
      361 gggatccatt ttgttgatgc agggaccagc ggcggcatct ggggattgca aaacggctat
      421 tgcctgatgg tcggcggtga aaaagctgtc gtcgatcatc tcgaaccgat cttgaaaacc
      481 ctggcgcctc ccgatggcta tttacatgtc ggaccggtgg gtgccggaca tttcgtcaaa
      541 atgattcaca acggtatcga gtacggcatg ctgcaagctt atggcgaagg gttcgagctg
      601 ctcaaagcct cgcagttcaa gctcgatttg ggcaaaattt cgcatctctg gaatcagggc
      661 agcgtcgtga gatcctggct cttgcagcta gccgaggacg ccttcaaaga ggatcctgag
      721 ctggcatcca tccggggcta tgtcgaggac tccggtgaag gacgttggac ggttgaagaa
      781 gcgatcgaaa aaggcgtgcc gacgccggtg ctgatgctct cgctcttcgc ccgttttgcg
      841 tcacgccagg aagattcttt ttccgcgaaa gtcatcgccg cgttgcgcaa tgaatttggc
      901 ggtcatgcgg taaagcgagg ctaaaaatat ggctcccgac ccgcacgatt atgttctgaa
      961 cacaggcgat ggcgccggcc cggcgaaaaa gccgcacgcc tgtgtgctgg tgatattcgg
     1021 cgcgtctggt gatctcacca aacgaaagct tattccggcg ctgtacaatc tggcgctcga
     1081 aaaccgcttg ccggaacgct tcgctgtggt cggttacgcc cgtagtgaaa tgagcgatga
     1141 tgtgtttcgc gaaaaaatgc gggatgctgt cagagagttt tcgcgcaccg gtttgcccaa
     1201 cgaatcggtc tgggcgcagt ttgctgcaac tctttattat gttcgcggcg gctatgaaca
     1261 aaacgacggg tttcgtaggc tgaaggagtt ggtcgagggc ttcgaccgcg gcagccgggt
     1321 attgcccgcg cgggtttttt atttggccac gccgccggac gcctacgagc aattgatcca
     1381 atgcattgct cgaaccggct tggcttccac cgaaagcgac ggtgagcagc gcgcacgggt
     1441 ggtcatcgaa aaacccttcg gtaccgacct gcagaccgcg cgtgagctca atcagcgcat
     1501 ccacgaggtg ttggacgagc ggcaaatcta ccgcatcgat cactatctcg gtaaggaaac
     1561 cgtacagaac atcatggtgt ttcgtttcgc caatgcggtc ttcgagccga tctggaaccg
     1621 ccgctacgtg gatcacgtac agatcaccgc ggcggaatcg gtaggcgtgg aaaatcgtgg
     1681 cggttattac gaacaagccg gcgtggtccg cgacatgttt caaaaccatt tactgcaact
     1741 gctctgcatg accgcaatgg aacctcccgt cggtctcgat gccgattccg tgcgcgatga
     1801 aaaaggcaag ctgctcaaat cggttcgacc cgttgccgcc gaagaagtcg ccgcagcagc
     1861 ggtccgcggg cagtatggac ccggcagggt ggatggtcag gaggtgcccg gctatcgcca
     1921 ggagcctggt gtggcgaaaa actcgtcgaa cgtcacctat acggcgattc ggtttttcat
     1981 cgataactgg cgctgggagg gcgtgccctt ttacctccgt tcgggcaaac gcatgccgaa
     2041 acgagtcagc gaagtcgcca ttcagtttaa acgaccaccg atgctgctct tcaaatcgca
     2101 cgccgttgaa gacgtgaacc cgaacgtcct ggtaatgcgc attcaaccgg acgagggcgt
     2161 ctctctgacg ttcgaagtta aacctcccgg acatgacatg atcattcgcc ccttatgcct
     2221 cgacttcgat tacgaggagt ctttcggcaa cagtccgccg gaagcttacg aaacgttatt
     2281 ggaagactgt atcgaggggg actcgactct ctttacgcgg catgactggg tggaattagc
     2341 ctggtcgctc gtggatccga tcattcaggt ctggaacctg agccgaccga gaaattttcc
     2401 caactacgaa gcgggttcct ggggtcccaa agaagccgac gaattcatgg cgcgcgaagg
     2461 acggcgctgg cgcaagccgt aaacacgagc ggaacagaaa ccgcccgacg gcggtgcaga
     2521 agttaaccgc gcacgcctgg aattgcggta agaatggaat tgaccaaaga gtaattcatg
     2581 tacccatcgg tacaacacac agcgcatgag aatgagccgt agtggttcgg acccatgccg
     2641 ataaaatccc ccactggccg cctttggcaa aggggcgggc cgtgggaccg ggggattttt
     2701 gcctctaatc ggaattttca taagagaaca cggagatcac gtacgttggt tggagtcttt
     2761 gacgacgtct acgtcaaccc tgcgtgtttt tcgtggtaag cgataatcca tgtctcataa
     2821 gctcgtcctc gccggcgata tcggcggtac taaaaccagc cttgcgttgt tttcggtcca
     2881 cggcgacaag cttcgcatcg aggcggagcg caatcttccg agcaagcagt actccggtct
     2941 gacgccgatt cttgaggaat tcggcgttca caagcaggtg atcgattcgg cctgcttcgg
     3001 gatcgccgga cctgtcgtcg acggtcgggt caaaaccacc aatcttccat ggatcatcga
     3061 cagccaagta ctttcccagg aacttaaagt cgagacggtt tatctgctca atgatttgga
     3121 agcgacagcc tatggtgtct tcactctaga ggacgacgaa ttctatcccc tcaaccaagg
     3181 catcctgcac cacaccggta acaaggcact gatcgccgcc ggcaccggct tgggacaagc
     3241 gattctctac gacgacggtc gccattttcg cgcgcttcct tccgagggtg gccacgccga
     3301 ttttgcaccc cgcgacgaac tggaaatcga gctgttacgc tatctcttcg ctcgctttgg
     3361 tcacgtgagc tacgagaggg ttttatcggg tccgggactc tttaatattt accgctttct
     3421 taaagagagc cgcggcatgg aagaaccact ctggctggcg gagcatttcg ccgccggcga
     3481 tgacccgggg gcgaccattt ccaatgcggc gctcgccggc aaagctgaga tctgtgtcaa
     3541 agcgctcgat ctgtttgtgt cgatctatgg cgcggaagcc ggcaatatgg cgctccgttt
     3601 taaggctgtc cggggactct acgttggcgg cggcatcgcg cccaaaattc tcgaaaagct
     3661 gaaggatggc acgttcatgc gctcctttat cgataaagga cgctacaccg aattgttaac
     3721 agcaatgccg gttcaagtcg tgttaaatcc gcaagccggt ttgcgcggcg ccggttatta
     3781 cgctgcgttc ctttctggca gttgagcccg cggtgtcatc cccccgacgc aattcgttgc
     3841 cgccgcgcaa cgccgctgta ttcggccgtt caccgtcgcc tagcggaggc tcgacgcctc
     3901 gcggtctatg cgcttctcgc cgcacacgaa tttatgagta aatcagtcca tctttttggg
     3961 gcagagctaa agctatttaa tttttgccag ctcccggcgg atctgttcgt tggacggtcg
     4021 cttcttgtag tcggtctcga tgtccttcca gcgtactacc ccttgaggat cgatcaggaa
     4081 cgtagatgga tgcggccaac cacggccgtc cggattgaaa acaccataac ggttgatcac
     4141 cttgtggtct ttgtcttcga gcagaggaaa atcgaactct ccctcgaatc gcttgctcag
     4201 catctcgcca aattttttcg actccgcgtg agtgtcgacg ctcaccgcga ggatttgagt
     4261 tttttccttc tgctgtttgg ttaaaaggct tttcagcttc ccgagctgtt cgacgcagaa
     4321 cggtcaccaa tagccgcggt aaaaaaccaa aacgacggtc ttcttgccgc ggaaatccga
     4381 taatgtgatc atgttgccgt cgacattttc caaggaaaaa tccggcgccg gctggccgac
     4441 cttgatccgg ttcaaatccg tcggcgcgag gtcggagccg tctttgggcc ccaactcagc
     4501 cagaacggtc gtgctgccaa ggcaaaatgc cagagcagca gctaagaaaa actgcgttcg
     4561 cataaaatca ctcctgttga aatttaatcg acgcccccgg tgccggtcat ggtcaacggg
     4621 tccacggttg acgctggcac tttgacgtag ttccggaatc ggcgctgcgc cagcgcgcga
     4681 aacaagcccg gcattacacg ggcgaaggcc gacaaaagcg ccagcgagcg cggcacgacg
     4741 acttcgaatt tatctttgtc gatcgcctcg atcacttctc ggcagaccgc ctcggtgctt
     4801 acgaccatcg ccggcggtgc tttaggttcg ctgtcgcgga aatcgcgatt gaaatccgtt
     4861 tgcaccggac ccggacagat caccgaaacc ttgatcccca gcggcttgag ctcgtggtag
     4921 aggctttccg accagccgtt gagcgcaaac tttgagccac agtaggcggc caggttcagc
     4981 gtgccgattt tgcccgcgcc ggaagaaata ttgacgatgt gaccgattct cctggcgatc
     5041 atcgacggca gcgcttcatg cgtgcagtag accgcgccga gataat
//
LOCUS       DASULZ010000669         2330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_989393, whole genome shotgun sequence.
ACCESSION   DASULZ010000669 DASULZ010000000
VERSION     DASULZ010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989393"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VFY96_13880"
     CDS             complement(<1..503)
                     /locus_tag="VFY96_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX5607608.1"
                     /translation="MKLRTVISFAAAVALNGFIASVATADVYAFRDKNGVVTFTNVPN
                     HAGFRRVFRDASRSATSNGVVDSGYDDYIQSASGRYNIDPDLIRAVIKAESDFKVSAR
                     SNKGAMGLMQLMPDTARLHNVSDAYNPGENVEGGARHLRMLLDRYQGDLELSLAAYNA
                     GSAAVEK"
     gene            complement(608..1150)
                     /locus_tag="VFY96_13885"
     CDS             complement(608..1150)
                     /locus_tag="VFY96_13885"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HEX5607609.1"
                     /translation="MTSATSAMRRQLCSAGSLDRTGGLLSQRAMNCSQKVKHVKPPSR
                     TAISVIAGLIFRNERILICQRRQNAAFASLKWEFPGGKIEDRETDTDALRRELKEELA
                     IDVRESEFFSGYVYSYEDGPTVSLRFHRVKSFQGEPQNLVFEQVSWVEVDDLVNFDFL
                     EGDRRLIMQMIERQDTFSKA"
     gene            complement(1147..1521)
                     /locus_tag="VFY96_13890"
     CDS             complement(1147..1521)
                     /locus_tag="VFY96_13890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5607610.1"
                     /translation="MPKKVLIIEDNRHLREILASILHSSGYLILQAATGLQGIETAVA
                     EQPALILLDLDLPDITGIETARVLKENPVTADIPIVACSAMSGSEWRDLALRTGIADY
                     LQKPISLAIIKAKIEQYTLFER"
BASE COUNT          559 a          621 c          554 g          596 t
ORIGIN      
        1 tgcttctcca ccgcggcgct cccggcattg taggcggcga gagaaagctc cagatctccc
       61 tgataacggt cgagtaacat tcgcaagtgc ctcgctcccc cttccacgtt ttcacccggg
      121 ttgtaggcat cgctgacatt gtgtagccgt gcggtatcgg gcatcagctg catgagtccc
      181 atcgctccct tattcgatcg agcgctgacc ttgaaatctg attctgcctt gatcaccgca
      241 cgaatcaagt ctggatcgat attgtaacgg cccgatgcgc tctgaatgta gtcgtcatag
      301 cctgagtcga ccactccgtt cgaggttgct gaacgacttg cgtcccggaa gactcgtctg
      361 aatcccgcat gatttgggac attggtaaaa gtcacgacgc catttttgtc tctgaaagca
      421 taaacatcag ccgttgctac actcgcgata aatccattca gcgccacggc tgctgcgaaa
      481 gaaatcacag tgcggagttt catatgttcg ggggctaaaa ttccgcatag gataaccgca
      541 ctatgaatgg ctgtcaaaca agcttgcgct ctgtgaaaga aagggcgagc cggtaagcga
      601 aacccagtta agccttggaa aaagtgtctt gccgctcgat catctgcata atgagacgac
      661 gatctccctc caaaaaatcg aaatttacga gatcgtcgac ctcgacccag gaaacttgct
      721 cgaatactaa gttttgcggc tctccctgga agcttttgac ccgatgaaag cgcagagaaa
      781 ccgtaggtcc gtcctcgtac gaatagacgt atccgctgaa aaactccgat tcacgaacat
      841 cgatggccaa ctcttccttc aactcgcgac gcaaggcatc cgtatcggtt tcccgatctt
      901 ctatttttcc gccgggaaac tcccatttca acgacgcaaa tgcggcattt tgacgacgct
      961 ggcaaatcaa aatcctttca tttcgaaaga tcagaccagc gataacactg atggctgttc
     1021 gcgacggtgg cttcacgtgt ttaacctttt gactacagtt catggctcgc tgagagagaa
     1081 ggccgccagt tcgatctaaa ctcccagccg aacacaactg tctgcgcatg gcagaggttg
     1141 cgcttgtcat ctctcgaaca gcgtgtactg ctcgatcttt gctttaatga ttgccagcga
     1201 aattggtttt tgtaagtaat cggcgatgcc agtgcgcagc gcgagatcgc gccattccga
     1261 cccgctcatt gcgctacacg cgacaatagg aatgtcggcc gtcactggat tttcttttaa
     1321 gacgcgtgcc gtctcaatcc cagtaatgtc gggaagatct aagtccaaaa gaatgagggc
     1381 gggttgttcc gccaccgctg tctcgatgcc ttgaaggccg gttgctgcct gcagaataag
     1441 atatccggaa ctgtgcagaa ttgaggcgag aatttcacgc aaatgtctat tgtcttcgat
     1501 aattagaacc ttcttcggca tgtccggcca ttctccgggc gaacaactgc tcaagcgacg
     1561 ccgcctagtt aatcatgttg aagtcgtaaa aggaacccca tagatccgat ctatcagcgg
     1621 tctccacgcg acgtagcaac gactctctcc cgattcacag cgagggccgt caggtcattt
     1681 cacctgagct catcaaaatt gataaccgta ttaccccctt gatgagcaga gtagcgaggc
     1741 cccgccgccg accggtagcg atattcgtcg aggatcgttt gaaggaatag tcgccggtta
     1801 tcgcaactcg gttacgacca attgggatcc agcgtcgctg accatagcta cttccgcggt
     1861 ttcggcaatt ttcgtcatct cttccgtcga gaagattttc tatggtcggt cgcagtgttt
     1921 gaactgtctc tcagccgtcg ttcaatgagc ttctttttca accgccactg attgccgacc
     1981 ttgaaagccg aaatctgtcc atccgtcacc aaacgataag tcgtgactta tccacgttta
     2041 agtattcggc tacttgcgcg acgcttagca gttgctcctg tctcattggg caatgtccta
     2101 gcgggtcaac aaaacttcgg gccgtatttg gtcgttacgt tactcgttga agagaagcga
     2161 tcaccggaga taatgcttgt tacactgtca aaatttattt aaccaaatac gggagccacg
     2221 tttattgaag catgccggtt taatcatcgt aagagacgct ggaggtttat gccgtttatc
     2281 gagctattct ctacaactca tgccaattta tgcttgtatt taccaagtag
//
LOCUS       DASULZ010000670         4028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_989541, whole genome shotgun sequence.
ACCESSION   DASULZ010000670 DASULZ010000000
VERSION     DASULZ010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989541"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..868)
                     /locus_tag="VFY96_13895"
     CDS             complement(14..868)
                     /locus_tag="VFY96_13895"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX5607611.1"
                     /translation="MMPLRKFTIHQPKTVTEASEMLAEFGDNARLYAGGTELLLTMKH
                     DLLRYQHLVDVKTIPELNEIQLKNGAVIIGSTATHRSIERSSIVKEHLPVFADMETHV
                     ANVRVRASGTLGGNLCFAEPHSDPATLLVALGAKAHIRGKSASKAIGIEQLIIGSYET
                     SLGPDEILASVEIPVLSKTQRAAYLKFQLKERPTLGLALVLDVEADQINKASAVVGSV
                     SASPTQSQNANQLLIGSRAEVEKQLANAGEALAEAADPVDDLEGSADYKRHLIGVFLR
                     RAFLKALS"
     gene            complement(968..3259)
                     /locus_tag="VFY96_13900"
     CDS             complement(968..3259)
                     /locus_tag="VFY96_13900"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5607612.1"
                     /translation="MSAEKKTDFQVVNHSLPRRDGRVKVTGKAQYVADLKLIGMAYAK
                     VLRSPYAHAKILSIDKSRAEAHPGVHCVVTGFDLDGINPYYGHAVKDHPLLAIEKVRY
                     AGEPVAAVVAIDERTAFEALELIEVKYEELKAIFTPHEAAAKDAPLLHQRQFEAGALR
                     GFEGDVTAGKGSNICQSHKIQWGDVEKAFKEAAAVIEGEYYFPMTYAYAMEPYVAVAD
                     VNNQEVTIYSSAQHPFMVRHDLKSVFNLPVSSVRLIVPFVGGGYGSKSYTKIEPLVAA
                     CSWKAKRPVKLQLTVEEAMLTTRSDDAFTWMRTAVDANGKIIARQAKIMMNTGAYAEN
                     SPLVVEKSTNRCVGPYAIPNVLIENSSYYTNTVPASSYRGFGCAQVTLPGESQIDELA
                     AKIGKDPYEFRLANAAKPGEEFFPGMRPFDGTLKEDLETAAKAVGWGKPLEKNHGRTV
                     AVSGSDAGAYPLTSTAIRVHADGSVTIMTGSTELGQGSHTVLPQIAAEELGVPFEKVR
                     VVSSDTAITPYDRSTGASRTTTLMGSAVLEASRDAIQQMVVMAADVLKVKPEQIQTVR
                     GGVRCGEAQLSWSEVICKFYALPDGEVIGRAYIRKSGKFSALPVFWEAAATGCEISVD
                     EETGKITMEKLATVGDVGLAINPALAEGQDIGAATMGMGIGLFEELVYEGAQLVNGSI
                     LDYRLPRFSDLPQHVELHLVQNQDGVGPYGAKGGGEASLNSMAANIANAVDRAVGVRI
                     RQAPLTPERVWRALKEKKSKMQR"
     gene            complement(3256..3735)
                     /locus_tag="VFY96_13905"
     CDS             complement(3256..3735)
                     /locus_tag="VFY96_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5607613.1"
                     /translation="MKLSFKLNGKAIALECEPADTLAEVLRNTLNLNGTKVSCEVQVC
                     GACTVLVEGVPVSACTYLAYEAREKNVTTIEGLERPDGTLHPLQQAFIDEFAFQCGYC
                     TPGMIMSAKALLDENPKPTRDEIIHHMDGNICRCTGYVPIIAAIERAAEAIGKESKK"
     gene            complement(3823..>4028)
                     /locus_tag="VFY96_13910"
     CDS             complement(3823..>4028)
                     /locus_tag="VFY96_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607614.1"
                     /translation="SYERDIDSMQFALDRHIFGSIDDVRRRVDEFIESGVQHFELKII
                     YPTMDSLHRQMELWAENILPRYN"
BASE COUNT          834 a         1131 c         1122 g          941 t
ORIGIN      
        1 tgaagacctg cacttacgag agcgctttta gaaaagctct tcgcaagaaa acgccgatca
       61 ggtgacgttt gtagtcggcg ctaccttcaa gatcgtcgac ggggtcagcg gcctcggcga
      121 gggcttctcc tgcgttagcc aattgctttt caacttccgc tcgggagccg atcaggagtt
      181 gattagcatt ttgagactgc gtcggcgaag cactaacact gccaaccacc gcagacgctt
      241 tattgatctg gtcagcttcc acgtcgagca ccagcgccaa acccaacgtt ggtcgttctt
      301 taagttggaa tttgaggtaa gcagcgcgct gggttttcga caataccggg atctcgacac
      361 tcgccagaat ctcgtccgga cccaacgatg tttcgtagga gccgatgatg agctgctcaa
      421 taccgatggc tttagaagcg gactttcccc ggatgtgagc cttggcaccg agggcgacga
      481 gcaatgttgc ggggtcggag tgtggctcgg cgaaacagag attgccgccg agcgttccgc
      541 tggcgcgtac acgtacgttg gccacgtgag tttccatgtc cgcgaatacc ggcaaatgtt
      601 ctttaacgat ggaagaacgt tcgatactcc gatgggttgc cgtgctgccg atgattaccg
      661 cgccgttttt caactgaatc tcattcagct caggaatcgt cttgacatcg acgagatgct
      721 gataacgcaa caggtcatgc ttcatcgtga gcaaaagctc cgtgccaccc gcgtaaagcc
      781 tggcgttgtc gccgaattcg gcgagcatct cggatgcttc ggtaaccgtc ttcggctgat
      841 ggatggtgaa ttttcgtaaa ggcatcatga cgattttaga ttttggatcc ttcgatttcg
      901 ctcaggacag gttttggatt ttcgactatt cggaaacagg actggcaaaa ctaccgctct
      961 tattacctca cctctgcatc ttggatttct tttccttcag tgcccgccag acacgttcag
     1021 gcgtcagcgg cgcctgccgg atacgcacac cgaccgcgcg gtcgacggca ttcgcgatgt
     1081 tggcagccat cgaattcaaa gaagcttcgc cgcctccttt cgcgccatag gggcccacac
     1141 cgtcttgatt ttgaaccaga tgaagctcga cgtgttgcgg cagatcggaa aagcgcggca
     1201 gacgatagtc caaaatggat ccgttgacga gctgcgcgcc ttcgtaaacc aactcttcaa
     1261 aaaggccaat gcccatgccc atcgtcgcgg cgccgatgtc ctgaccttcg gccagcgccg
     1321 gattaatcgc gagaccgaca tcgccaaccg tggcgagttt ctccatagtg attttaccgg
     1381 tctcttcatc gacggaaatt tcgcagccgg tcgccgcggc ttcccaaaat accggcagag
     1441 cggaaaactt gccggattta cgaatatagg cgcggccgat cacttcgcca tcggggagcg
     1501 cgtagaactt gcaaatgacc tccgaccagc tcaactgcgc ttcaccgcaa cgcacacccc
     1561 cgcggacggt ttggatctgt tcgggcttca ccttcaatac gtcggctgcc atcacgacca
     1621 tttgctgaat cgcatcccga ctcgcctcga gtacggcgct acccattaaa gtcgtggtgc
     1681 ggctcgcgcc cgtggaacga tcgtaggggg tgatcgcggt atcggaagag actacgcgga
     1741 ctttttcgaa gggcacgccg agctcttcag cggcgatctg cggcaggacg gtgtgactac
     1801 cctgacccaa ttcggtgctt ccggtcatga tcgtcacaga accgtcggca tgcacgcgaa
     1861 tcgcagtgga ggtcagcgga taggcgccgg catcgctgcc ggagaccgca actgtgcggc
     1921 catgattctt ctctaggggc ttgccccaac cgactgcttt tgctgcggtc tccagatcct
     1981 ctttgagggt tccgtcgaag ggccgcatcc ctgggaaaaa ttcttcgccc ggctttgccg
     2041 cgttggccaa gcgaaactcg tatgggtcct tgccgatctt ggcggcgagc tcatcgatct
     2101 gcgattcgcc cggcagtgtt acttgagcgc agccaaagcc gcgataggag ctggccggta
     2161 cagtattcgt gtagtaggac gagttttcga tcagcacgtt gggaatcgcg tacggcccga
     2221 cacagcgatt ggtcgatttc tcgaccacca gcggactgtt ctccgcatag gcgccggtgt
     2281 tcatcatgat ctttgcctgg cgcgcgatga tcttgccgtt agcatcgacc gcggtgcgca
     2341 tccacgtgaa agcatcgtcg ctgcgcgtcg tgagcatcgc ttcttccacg gtcagctgca
     2401 atttcaccgg tcgcttcgcc ttccacgaac aggccgcgac cagtggctcg attttggtgt
     2461 acgatttgct gccgtaaccg ccgccgacaa agggcacgat cagtcgaacg gagctgacgg
     2521 gcagattgaa aactgatttc aggtcgtggc gcaccatgaa cggatgctgc gccgatgaat
     2581 aaatcgtcac ctcctggttg ttgacatcgg cgacggcgac gtaaggttcc atggcgtagg
     2641 cataagtcat aggaaaataa tattcgcctt cgattaccgc cgcggcttct ttgaaggctt
     2701 tttcgacgtc gccccactgg attttatggc tttggcaaat gttcgagcct ttgccggccg
     2761 tgacatcgcc ctcgaagcca cggagcgcgc cggcttcaaa ctggcgttgg tgcagcagcg
     2821 gcgcgtcttt tgcggccgct tcatgcggcg tgaagatcgc tttcagctct tcgtatttga
     2881 cctcgattaa ctccagcgct tcaaaggcag ttcgctcgtc gatggcgacg accgcggcga
     2941 ccggctcgcc ggcataacga actttctcga tggcgaggag cgggtgatct ttgactgcat
     3001 ggccgtaata aggattgatg ccatcgagat cgaaaccggt gaccacacag tgaaccccgg
     3061 gatgcgcctc ggcccgagac ttgtcgatgg aaagaatctt cgcgtgtgca taggggctgc
     3121 gcagcacctt cgcgtaagcc atgccgatga gtttaagatc agcgacgtat tgcgctttgc
     3181 cggtcacttt cacgcgacca tcgcgacgcg gcaacgaatg gttgaccacc tgaaagtcgg
     3241 tttttttctc ggcgctcact ttttcgactc cttaccgatc gcttcggcgg cacgctcaat
     3301 tgcggcgata atcggcacat agccggtgca gcgacaaata ttgccgtcca tgtgatggat
     3361 gatttcatcg cgcgtcggct taggattctc gtcgagcagc gccttggcgg acatgatcat
     3421 tccgggagtg caatagccgc actgaaaagc gaattcatcg atgaaggcct gctgcagcgg
     3481 gtgtagcgta ccgtcgggtc tttcgaggcc ttcgatggtc gtgacgttct tctcgcgcgc
     3541 ttcgtaagcg agataagtgc aggcgcttac cgggacgccc tcgacgagca cggtacaggc
     3601 gccgcaaacc tgcacttcac aggacacttt ggtgccattg agattgagcg tattgcgcag
     3661 tacttcggcg agggtgtcgg caggttcgca ctcaagcgca atcgcttttc cgtttaattt
     3721 aaatgacagt ttcataattc caattggagt gctggcatga tggagtattg gagtgatggg
     3781 cttggaatca gagcattact ccgccaataa tcacactcca tttcaattgt atctcggcaa
     3841 aatgttttct gcccagagct ccatttgccg gtgcaagcta tccatcgtcg ggtagataat
     3901 cttcagctca aaatgctgca cgccactttc gataaattca tcgacgcgcc ggcgcacgtc
     3961 atcgatggaa ccgaaaatat gacggtcgag ggcaaattgc atgctgtcga tatcccgctc
     4021 atagctct
//
LOCUS       DASULZ010000671         2322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1396836, whole genome shotgun sequence.
ACCESSION   DASULZ010000671 DASULZ010000000
VERSION     DASULZ010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396836"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VFY96_13915"
     CDS             complement(<1..279)
                     /locus_tag="VFY96_13915"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF013575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HEX5607615.1"
                     /translation="MNIKLTVEYDGTNYCGWQTQPNGRSIQSVLEEAVSTFLGTPTRV
                     IGSGRTDAGVHALGQVANFFCDGEFDRHRLLRGLNALTPRDITIKEAEI"
     gene            complement(485..1507)
                     /locus_tag="VFY96_13920"
     CDS             complement(485..1507)
                     /locus_tag="VFY96_13920"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX5607616.1"
                     /translation="MKKEKYNVAVVGATGAVGEQMREVLEEREFPVGELRLLASERSA
                     GQFLPFDGRQIRVEVLSEDSFDGIDIGLFSAGGSVSAKFAPISVAAGAVVVDNTSCFR
                     MEPDVPLVVPEVNAKEIAHYKNRGIIANPNCSTIQMVVVLKPIHDAARIKRVVVSTYQ
                     SVSGAGRKAMEELSQQVAALFNGKEIEKQKFPHQIAFNCIPHIDAFVENGYTKEEIKM
                     INETRKILSEPNLPITATTVRVPVFCSHSESVNVETIVKLSAAETKRILRESPGVILA
                     DVPEENIYPMAIDAVGKDATYVGRIREDTSVPNGLNLWVVSDNLRKGAALNAVQIAEI
                     LIRDYI"
     gene            complement(1587..>2322)
                     /gene="leuB"
                     /locus_tag="VFY96_13925"
     CDS             complement(1587..>2322)
                     /gene="leuB"
                     /locus_tag="VFY96_13925"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017306459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX5607617.1"
                     /translation="ASTLKPQVVEGTDLLVVRELTGGIYFGQPKGVTKLADGTERGVN
                     TEVYTTPEIERIAHVAFQAALKRRKKVTSVDKANVLEVTELWRKVVTRIHKDESYASV
                     QLEHMLVDNCAMQLIRNPKQFDVIVTTNMFGDILSDEAAMLTGSIGMLPSASLGGKVG
                     MYEPVHGTAPDIMGQDKANPLATILSVALMLRHSFDQSDAADRIEKAVEEVLNQGWRT
                     ADIHESGGKVVGCREMGRLVRKKMDN"
BASE COUNT          518 a          686 c          563 g          555 t
ORIGIN      
        1 gatctcagct tccttgatcg tgatgtctcg cggtgtaagg gcatttaagc cacgtaataa
       61 gcgatggcgg tcaaactcgc catcgcaaaa gaagttcgcc acctgaccca gcgcatgaac
      121 accggcgtcg gtgcgacccg agccgattac gcgcgtggga gttcccagga aagtcgagac
      181 cgcctcttcc aaaaccgatt gaatcgagcg cccattaggc tgggtctgcc agccgcagta
      241 attcgtgccg tcgtattcaa ccgtgagctt gatattcacg acagactagt ttcaggtttc
      301 gggatgcgga ggcgtggagt tcacagagac gttcacggtt tgtacgaaat ctcaacacga
      361 agtgattcat gggccttaca ttcgccgaca aaataaaaac ggcccgggga aagattcctc
      421 gcagcctgct cgcaataaca atatgtcatg cctcgcgagc ttagcgtcct tcatggtgca
      481 gttcctatat gtagtcgcga atcaatatct cggcgatctg caccgcgttc agcgctgcgc
      541 ctttccgcag gttgtccgat accacccaaa gattgagacc gttgggaacc gatgtatcct
      601 cgcgaattct ccccacataa gtggcgtctt tcccgaccgc atcgatggcc atcggataga
      661 tattctcctc cggcacgtcc gccaaaatca cgcccggaga ctctcgcaga attcgcttgg
      721 tctcggccgc cgaaagcttg acgattgttt ccacgttcac cgattccgag tgactgcaga
      781 ataccggcac ccgcaccgtg gtcgccgtga tcggcaaatt cggctcgctc agaatcttgc
      841 gcgtctcatt gatcattttt atttcttctt tggtataacc attttccaca aaagcatcga
      901 tatgcgggat gcagttgaaa gcgatctgat gcggaaactt ctgcttttcg atctccttgc
      961 cattgaatag cgccgctacc tgctggctca attcctccat ggccttgcgc cccgcaccgg
     1021 agacagactg ataggtcgaa acgacaactc gcttgatccg cgccgcatcg tgaatcggtt
     1081 tgagcacgac gaccatctga atggtcgagc agtttgggtt ggcaatgatt ccgcggtttt
     1141 tatagtgcgc gatttctttc gcgttcactt ccgggaccac cagtggaacg tcgggctcca
     1201 tacgaaagca ggacgtattg tccacgacca cggcgcctgc tgcgactgag atgggcgcga
     1261 atttggcgct gacgctgcct cctgcggaaa aaagcccaat atcgattccg tcaaacgaat
     1321 cttcacttaa aacttcaacc cgaatctgcc gcccatcgaa gggcagaaat tggccggcgg
     1381 atctttccga cgccagtagc cgcaattcac cgacgggaaa ctcacgctct tccaaaactt
     1441 cacgcatctg ctcgccgacg gcgccggtcg cgccaacgac agcgacattg tatttttctt
     1501 ttttcatcaa gttctcagca gtagttcaca aactcaagac caagatcgac agagtcgatc
     1561 ttaaaacgat gctcgatctg ctatgctcaa ttatccattt ttttacgcac caagcgtccc
     1621 atttcacgac agccgaccac tttaccgccc gactcgtgaa tatcggcggt gcgccagcct
     1681 tgattcagca cttcttcgac ggctttctcg atccgatccg cggcatcgga ttgatcaaag
     1741 gaatgccgca acatcagcgc cacgctcaag atggtcgcca agggattggc tttatcctgg
     1801 cccataatat ccggcgccgt gccatgcact ggctcgtaca tgcccacctt accgcccagg
     1861 ctcgcggacg gaagcatgcc gatcgaccca gtgagcatcg ctgcctcatc gctcagaatg
     1921 tcgccgaaca tattggtggt aacaatgacg tcgaactgct tgggatttct gatcaactgc
     1981 atagcacagt tgtcgaccaa catgtgttcc agttgcacac tggcataact ctcgtcctta
     2041 tgaatccgcg tcaccacttt acgccagagt tctgtaacct cgaggacatt tgctttatcg
     2101 acggatgtca ctttcttgcg ccgcttgagc gccgcttgaa aagcgacatg tgcaatccgt
     2161 tcgatctccg gcgttgtata cacttccgta ttgacgccgc gctcggtccc gtccgctaac
     2221 tttgtcacac ctttcggctg gccaaagtaa attcctccgg taagctcgcg cacgaccagg
     2281 aggtccgtgc cttcaacgac ttgaggcttc agagttgagg cc
//
LOCUS       DASULZ010000672         7007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1089991, whole genome shotgun sequence.
ACCESSION   DASULZ010000672 DASULZ010000000
VERSION     DASULZ010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7007
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089991"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="VFY96_13930"
     CDS             complement(<1..931)
                     /locus_tag="VFY96_13930"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEX5607618.1"
                     /translation="MIVVTAAQMRELDRLTIEKYGVPSLTLMERAGHAIAHAMIERFS
                     RYAKKGVLVVAGKGNNGGDGFVVARLLKKKRIPCEVALLARRDELSPDAAHNLNAFVK
                     LKGKITEITMADVGLLGERARKTGLLVDAILGTGVKNEVRGLYADAITSINGCGVPVM
                     AVDIPSGIHTDSGVPLGVAVQSEMTVALGFAKRGEVIYPGIDYVGALIVADIGIAAQA
                     VAEVNPDTELLERETVRRLVPRRGADTHKGSYGHVLVVAGSRGKTGAAILASRAAMRS
                     GAGLTTLAAPRSLNNIFASSLIEVMTEPLGENTA"
     gene            complement(1043..1765)
                     /locus_tag="VFY96_13935"
     CDS             complement(1043..1765)
                     /locus_tag="VFY96_13935"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006851994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX5607619.1"
                     /translation="MIRLGVNVDHVATVRQARRAEHPDPVEAALLAEKAGADGITVHL
                     REDRRHIQERDVERLRFKIRTKINLEMAVTPAMVAFAERLRPAAACFVPEKREELTTE
                     GGLDVVAHEAKVIDAVRTLQSCGVQVSLFIDPVKEQIECAKRTGAHAIEIHTGTFCNA
                     NVSARDQERATIVNAAALAANLRLEVHAGHGLDYENVLPIAQISDIVELNIGHSIIAR
                     AVMVGIEQAVRDMKELLASVRG"
     gene            complement(1769..3220)
                     /gene="glmM"
                     /locus_tag="VFY96_13940"
     CDS             complement(1769..3220)
                     /gene="glmM"
                     /locus_tag="VFY96_13940"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX5607620.1"
                     /translation="MSQPQLKTQMPQRHLFGTDGVRGVANQEPMTSEMALKLGRAIAS
                     VLHDPVGDENSHRVSLPKFARKNGADGTHKYKILIGKDTRLSGYMLETALASGIVSMG
                     VDVVLVGPMPTPGVAFLTRSMRADAGVVISASHNPYQDNGIKFFSCDGFKLPDEVEAR
                     MEEMIFNETTGQSRPTASEIGKAFRLSDAVGRYNVFLKNTFPRHLTLEGLKIVVDCGH
                     GAAYKVVPEVLSELGAEVLPIGVQPDGENINRRCGALFPETAREVLLHERADFAVSLD
                     GDADRAMFIDETGDILDGDQVLAIAALDMQQKGILTGSGVVATVMSNLGLELALRNAG
                     LEVVRTQVGDRYVVEKMLSGSYNLGGEQSGHILFLDHNTTGDGAITCLQMLALMVETR
                     KKLSELKCVMTRLPQILLNIHVKEKKDLSAMPKVSQKIAEVERTLAGRGRVLVRYSGT
                     ELLARIMLEGEDEKKISAMANAIADEIRTEVGR"
     gene            complement(3243..4172)
                     /locus_tag="VFY96_13945"
     CDS             complement(3243..4172)
                     /locus_tag="VFY96_13945"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HEX5607621.1"
                     /translation="MKQVWERVVSWSGSNFGLKVLALIIALGLWLAGHRDTERAIEVP
                     VEFRNIPQDLMVMDNRMEYVVLRLMGPRTLVSTLDAQTLKLSLDLGGAKSGSASFPLG
                     ADDFNVPRGVAIGRITPPVIHLRLEPVVKRTLPVTVRLSGKPAAGYMIAETSANPRTV
                     AVQGPAEEVRRLSTIDTISVDVEDSRSTIKRKVRLTTDGKPLTISPDQVDVAVNVEEQ
                     QIVREFDRIEVQAKDFKGAYTISPQWVKLRLAGPKSIVEKLDLTAENVYLDLHGMSAG
                     DHNLELSFNLPPEIRVVEQRPQRVRVRIVKPAA"
     gene            complement(4169..4927)
                     /gene="cdaA"
                     /locus_tag="VFY96_13950"
     CDS             complement(4169..4927)
                     /gene="cdaA"
                     /locus_tag="VFY96_13950"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEX5607622.1"
                     /translation="MLELIRQIRWQDALDVGIITFLVYRALQIVRGSRAMQMIIGLAV
                     ILVAYVSSRALGLFTLNWILDNFLGSIILVIIVIFQSDIRRALTQVGTTPLFTGGERM
                     AQRREDILEEIVQAAMSLAEKRIGGLIVIQREVGLNEYMEIGTRLDARVSRELVESVF
                     SPHSPIHDGALVVQKGRVTAVRCLLPLSTNPNLRKTWGTRHRAALGVTEETDCVAIII
                     SEQEGTVALVVGGNVTDNINGARLRLALQDLIKA"
     gene            complement(4930..5853)
                     /gene="folP"
                     /locus_tag="VFY96_13955"
     CDS             complement(4930..5853)
                     /gene="folP"
                     /locus_tag="VFY96_13955"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX5607623.1"
                     /translation="MADKKQQPIVTRVPRPALFLSPEERILPTRHSVLEFSRRTLIMG
                     IINVTPDSFSDGGQRYDAVKAVADGVAMVKSGADILDIGGESSRPGATPVSEDEELAR
                     VLPVVRGLRKKTEAPISIDTYQSNVARAALDAGADIVNDISALRFDPAMAPMVAAEKV
                     PVVLMHMQGTPRTMQREPKYADVVREVRDFLAAQLYTAMDAGIAQDRIVIDPGIGFGK
                     TLQHNLQLLRGLPMIAALGQPVLVGASRKAFIGRILNAEPLDRIEGSLATAVASVLAG
                     ANIVRAHDVAETAKAVKIADAIRFGYSPLPA"
     gene            complement(5997..>7007)
                     /locus_tag="VFY96_13960"
     CDS             complement(5997..>7007)
                     /locus_tag="VFY96_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5607624.1"
                     /translation="NQLLVEMDGFEANEGVILIAATNRPDVLDPALLRPGRFDRRVVV
                     PRPDVKGREGILQVHTRKVPVGTDVDIGVLARSTPGFAGADLENLVNEAALLAARNNK
                     EKVDMRDFELAKDKVMMGAERRSMIISDEEKRNTAYHEAGHALVAKLLPGADPIHKVT
                     IIPRGMALGLTQQLPMDEKHTYPREYLLNNLVILFGGRVAEELVLNHMTTGAGNDIEK
                     ATDLARRMVCEWGMSEKLGPMTFGKKEEEIFLGRDFTQKVDYSESTAIEIDAEVRRII
                     QDSYHKAKELLKTNLRLLHKVAESLLEKEVLDGSEIDAIVREFGGNGGDPLASSAAAA
                     VA"
BASE COUNT         1474 a         2112 c         1857 g         1564 t
ORIGIN      
        1 cggcggtgtt ctccccgagc ggctcggtca tgacttcgat caacgaactc gcaaaaatat
       61 tgttcagtga acgcggcgca gccaaggttg tcaatcccgc gccgcttcgc attgccgcgc
      121 gcgacgccag gatcgccgcg ccggttttac cgcgcgatcc ggcaaccacc agcacatggc
      181 cgtaacttcc tttgtgtgtg tcagcgccgc gccgcggcac caaccggcgc accgtctcac
      241 gctccagcag ctcggtatcg ggattaactt ctgcaaccgc ttgagcggcg attccaatat
      301 cggcgacgat caacgcaccg acatagtcga tgcccgggta aatcacttcg ccgcgcttcg
      361 caaacccgag tgcgactgtc atctccgact gtacggcgac acccagcggg actccgctat
      421 cggtgtgaat gcccgaggga atgtccacag ccatcaccgg cacaccgcaa ccattgatcg
      481 aagtaatcgc gtcggcataa agaccccgca cctcgttttt gacgccggtg ccgaggattg
      541 cgtccaccag gaggccggtt ttgcgagctc gctcgccaag cagaccgaca tcagccatcg
      601 tgatctcggt tatttttcct ttgagcttta caaaagcgtt taaattgtgg gctgcatccg
      661 gtgaaagctc gtctcggcgc gccagcagag ccacctcgca tggaatgcgt ttcttcttga
      721 gcaagcgcgc cacaacaaaa ccatcgccgc cgttattgcc cttcccggct acaaccaaga
      781 cccccttttt ggcataccgc gaaaagcgct cgatcatcgc gtgcgcaatg gcgtgcccgg
      841 cgcgctccat taaagtcaga ctcggcacgc catatttttc aatcgtcaac cgatccagct
      901 cgcgcatctg tgcagcggta accacaatca tggttcaaaa tggttcaagc agttcaaacg
      961 gttaaaatcg ttccaacggt tggaactgag cgcagcggtt gaacggaacg agcaaagcga
     1021 gtgattgaac gacttgaatc tcttagcctc ttacgctagc tagcagttct ttcatatcgc
     1081 gcaccgcctg ctcgatgccg accatgacgg cgcgagcgat gatgctgtgg ccgatgttca
     1141 gctcgacaat gtcggaaatc tgcgcgatcg gcagaacgtt ttcatagtcg agaccatggc
     1201 cggcgtgcac ttccagtctc aggtttgccg ccaaagctgc tgcattcacg attgtggcac
     1261 gctcctgatc acgcgcggac acattggcat tgcaaaaggt tccggtgtga atttctattg
     1321 catgagcgcc ggttcttttt gcgcactcga tctgttcctt gaccggatcg atgaacaaac
     1381 tgacttgaac gccgcaactc tgaagtgttc gtaccgcgtc gattactttc gcctcatgcg
     1441 ccacgacgtc cagcccaccc tcggtggtga gctcttcccg tttctccggc acgaagcagg
     1501 cggcagcggg ccgcaatcgc tcggcaaacg ccaccatggc cggcgtaacg gccatctcga
     1561 gattgatttt agttctgatt ttgaaacgca gtcgttcgac gtcgcgctcc tgaatgtggc
     1621 ggcggtcttc acggaggtgg acggttatac cgtcggcgcc ggctttctct gcaagcagcg
     1681 ctgcttcaac gggatccgga tgttccgcgc gccgcgcctg gcgcaccgtc gccacgtgat
     1741 cgacgttgac gcctagccgg atcatgatct atcggccgac ctctgtccgg atctcatcgg
     1801 cgattgcgtt cgccatggcg gagatttttt tctcgtcttc gccttcgagc ataatgcgcg
     1861 ctagtagctc ggtgccggaa tagcgtacga ggacgcgtcc gcgccccgcc aaggttcgct
     1921 cgacttcggc aattttttgg ctcaccttcg gcatcgctga aagatctttt ttttctttga
     1981 catgaatatt gagcaagatt tgtggtaacc gggtcatgac gcacttgagc tcgctcagtt
     2041 ttttgcgggt ttcgaccatg agcgcaagca tctgcaaaca ggtgatcgcg ccgtcaccgg
     2101 tagtgttatg gtccaaaaac aaaatatgac cggactgctc gccgccaaga ttgtaactgc
     2161 ccgaaagcat tttctcgacg acgtaccggt cgccaacctg ggtccgcaca acttccaatc
     2221 cggcatttcg caaggccagc tccaacccca gattgctcat gaccgtagcc accacgccgg
     2281 atcccgtcaa gatgcccttc tgctgcatgt ccagcgcggc aatcgccaaa acctgatcgc
     2341 cgtcgagaat atcgccggtt tcgtcgatga acatcgcgcg atcggcatcg ccatcgagtg
     2401 aaactgcgaa atctgctctc tcgtggagta aaacctcgcg cgcggtttcc gggaaaagcg
     2461 cgccgcagcg tcgattgatg ttctcgccgt cgggttgtac gccaatcggc agcacctcgg
     2521 cgccaagctc cgataatact tctggaacca ctttgtatgc ggcgccgtga ccgcaatcga
     2581 cgacgatctt caatccctcg agggtcaaat ggcgcgggaa agtgtttttg agaaagacat
     2641 tatagcggcc caccgcgtcc gaaagacgaa aagccttgcc gatttctgat gcggtgggac
     2701 gcgactgacc ggtggtctcg ttaaagatca tctcctccat gcgggcttcc acttcgtcgg
     2761 gcagcttgaa accgtcacag gaaaaaaact tgattccatt gtcctggtaa ggattgtgtg
     2821 atgccgaaat gacgactccg gcatccgccc gcatgctgcg cgtcaagaaa gcgacgccag
     2881 gagttggcat aggtcccacc agcaccacgt ccacgcccat tgacacaatg ccggaagcca
     2941 aagctgtctc cagcatgtat cctgacagac gcgtatcttt gccgattaga attttgtatt
     3001 tgtgcgtgcc gtctgcgccg tttttgcggg cgaattttgg cagggatacg cgatgcgaat
     3061 tctcatcccc taccggatca tgtaatacgc tggcgatggc gcggccgagc ttcagcgcca
     3121 tttccgaggt catgggttcc tggttggcga ctccacgaac tccgtcggtt ccgaaaaggt
     3181 ggcgttgggg catctgggtc ttcaattgcg gctgactcat ccaataaact cctttaacct
     3241 gctcaggcgg cgggtttgac aatccgcacg cggacccgct gcggccgttg ctcgacaact
     3301 cttatctcag gcggaagatt gaacgataac tcaaggttgt gatcacccgc tgacataccg
     3361 tgcagatcta ggtagacatt ctccgctgtc aaatccagtt tttcaacgat acttttgggt
     3421 ccggcgagcc ggagcttaac ccattgcggt gaaatcgtgt acgccccctt gaaatccttc
     3481 gcctggacct cgattcggtc aaattctcga acgatctgct gctcctcaac attgacggcc
     3541 acatcgactt gatctggcga tatggtgagc ggcttaccgt cggtggtaag acgaactttt
     3601 cgtttgatag tgcttcggct atcctcgaca tcgacagata ttgtgtcgat cgtcgacaat
     3661 cgccttactt cttcggccgg tccctgcacc gcaaccgtcc tagggttcgc cgaggtctcg
     3721 gcgatcatgt aacccgcggc gggcttgccg gataggcgca cagtgaccgg gagcgttcgt
     3781 ttaaccaccg gttctaagcg aagatgaatg accggcggcg ttattctgcc gatcgccacg
     3841 ccacgcggca cattgaaatc atcggcgccc aggggaaaac tcgccgagcc cgacttagct
     3901 ccaccaaggt ccaatgaaag cttcagcgtt tgcgcgtcga gggtggatac taaagtccgg
     3961 gggcccataa gccgtagcac cacatactcc atgcggttgt ccatgaccat caggtcttgc
     4021 ggaatgttgc gaaattccac gggcacttct atcgcccgct cggtgtcgcg gtgtcccgcc
     4081 aaccaaagtc ccagggcgat gatcaacgca agcaccttca gtccgaaatt gcttcccgac
     4141 catgaaacga cgcgttccca aacttgtttc atgctttgat caaatcctgc agcgccaagc
     4201 gcagccgcgc gccgttaatg ttgtccgtca catttccgcc aacgaccaaa gcgaccgttc
     4261 cctcttgctc agagataatg atcgcgacac agtcggtctc ttcggtcaca cccagcgcag
     4321 cccgatgccg tgtgccccag gtttttcgca gattcgggtt ggtgctgagc ggtaaaaggc
     4381 agcgcacggc agtgacccgg cccttttgga ccaccagcgc gccgtcatgg atcggcgaat
     4441 ggggtgaaaa aacgctttcc acaagttccc ggctcacgcg cgcgtcaagc cgcgtaccga
     4501 tctccatata ttcgttcaag cctacttctc gctgtatgac gatgagaccg ccgatgcgtt
     4561 tttccgccaa cgacattgcc gcttggacga tctcttcgag aatatcctcg cgccgctgcg
     4621 ccatgcgctc gccgccggtg aaaagcggcg tcgttcctac ctgcgtcaga gcgcgccgaa
     4681 tatcactttg aaagatgacg ataataacaa gaataatcga gccgagaaaa ttatcgagaa
     4741 tccagttcag cgtgaatagg ccgagcgccc gtgacgatac ataggcgacc agaatcacgg
     4801 ccaagccgat gatcatctgc atggcacggc tgccgcgaac gatttgcagg gctcgataga
     4861 caagaaaggt gatgatgccg acgtccagtg cgtcctgcca acggatctgc cggattaatt
     4921 ctagcatcgt caggccggca gcggagaata gccgaagcgg atggcatcgg caatttttac
     4981 tgccttggcg gtttcggcga cgtcgtgggc ccggacaata ttcgctccgg ccaataccga
     5041 agcgaccgca gtcgccaggc tgccctcgat tcgatcaagc ggttctgcat tcaaaattct
     5101 gccgataaat gccttgcgcg aagcgcccac gagcacaggc tgaccaagag cagcaatcat
     5161 cggcagccca cgcaatagct gtaagttgtg ctgcagggtt ttgccgaaac cgatccccgg
     5221 atcgatcacg atcctgtctt gggcaatgcc agcgtccatc gcagtgtaaa gctgtgcagc
     5281 gagaaagtcg cgcacttcac ggaccacatc tgcatatttc ggctcgcgtt gcatcgtccg
     5341 gggtgttccc tgcatgtgca tcagcacgac aggcactttc tccgcggcga ccattggggc
     5401 catcgccgga tcgaagcgca atgcgctgat gtcattcacg atatccgcac cggcatccag
     5461 cgccgcccgg gcgacgttgc tctgataggt atcgatggaa atcggtgcct cggtcttttt
     5521 gcgcaaaccg cgcacaaccg gcaacactcg cgcgagttct tcatcttcgg aaaccggcgt
     5581 tgccccgggg cggctggact caccgccgat atcgagaatg tcggcgccgc tttttaccat
     5641 cgcgactccg tcggcaacgg ctttgaccgc atcgtaacgc tgcccgccat ccgaaaagga
     5701 atccggcgtc acgttgataa ttcccatgat cagcgtacgt cgggaaaact cgagcacgct
     5761 gtgacgtgtc ggtaagattc gttcctcagg ggataagaac agcgcaggac gcggaactcg
     5821 agtcacgatc ggttgctgtt ttttgtcagc catccctatt gcgctggcgc tgcagagaaa
     5881 tcagcgccaa agctgatgcg ttggagcgtc tcccgaatag aagccgatcc gccaccccgt
     5941 ttacaatcgt ggtttagcgt ggaggaaggg agccaaaagc cccggcgaga aataaattaa
     6001 gcgacggccg ctgcggccga ggaagcgagc gggtctccac cgtttccgcc aaactccctg
     6061 acaatcgcgt cgatctcgga tccgtccaga acttcttttt cgagcagtga ttcagcaact
     6121 ttgtgaagca gccgcaaatt ggttttcaga agctccttgg ccttgtgata gctatcttga
     6181 atgatccgtc tgacttcggc atcgatctca atggccgtgc tttcggaata atcgactttc
     6241 tgcgtgaagt ctctgcctaa gaatatctct tcctcttttt ttccgaacgt catgggtccg
     6301 agtttctcac tcatacccca ctcacagacc atgcgccgtg ccagatccgt cgctttctcg
     6361 atgtcgtttc cagctccggt ggtcatatga ttgagcacga gctcctcggc gacgcgaccg
     6421 ccaaagagaa tcaccaggtt attgagcaag tattcccgcg gataagtatg cttctcgtcc
     6481 atcggcaact gttgcgtcag tcccaacgcc atgccgcggg gtataatcgt aaccttgtga
     6541 atgggatccg cccccggcaa cagtttcgcg accaaagcat gccccgcttc atggtaagcg
     6601 gtgttgcgct tctcttcatc gctgatgatc atgctgcggc gctccgcgcc catcatcact
     6661 ttgtctttgg ccagctcgaa gtcgcgcata tcgacttttt ctttgttgtt gcgcgctgcc
     6721 aaaagagctg cctcgttcac cagattttcg agatcggcac cggcgaaacc cggagtcgat
     6781 cgcgccagta caccgatatc gacgtcggtg cctaccggca ctttccgcgt atgcacctgc
     6841 agaatcccct cgcgcccttt gacatccggc cgaggcacga ccacacggcg atcgaaccgg
     6901 cctgggcgca gcaaagcggg atccaaaaca tcaggccgat tggtggcagc aattagaatc
     6961 acgccttcat tggcttcgaa cccgtccatt tccaccagca gctggtt
//
LOCUS       DASULZ010000673         3272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1193506, whole genome shotgun sequence.
ACCESSION   DASULZ010000673 DASULZ010000000
VERSION     DASULZ010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193506"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VFY96_13965"
     CDS             complement(<1..632)
                     /locus_tag="VFY96_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5607625.1"
                     /translation="MPITLPRSLLFIALVLLALFPLTGSTFYIQLGAKILIMAIFALS
                     LDLLVGHTGLVSFGHAAYFGLAAYALALISPKYEAANVWLTLPLAMGIAAFAALVIGF
                     FVLRTTGVYFIMVTLAFAQMLYSVFHDTRLGGGSDGLYIYVRPELTIAGFKPLDLESP
                     LQFYYFVLILLILVYGLLKRILHAPFGRALAGIRLNEQRMRSIGFPIFGY"
     gene            complement(641..1513)
                     /locus_tag="VFY96_13970"
     CDS             complement(641..1513)
                     /locus_tag="VFY96_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5607626.1"
                     /translation="MNLLNMFYLIQLLNAVQYGLLLFLLSSGLTLIFGIMGVINLAHG
                     SFYMVGAYLAYWLAKLTGNFWLAIPIGTVVTLGIGGLLETTIIRRLYRRDHLYQVLST
                     FGLILIFEELRSILFGNDVHGVTIPAGLNYSIPLTDTLSYPVYRLFVTGVCLITAALL
                     YWVIYKTRVGMMIRAGNSNREIAASLGVNVPLLFAWVFAAGTALAGFAGAVAAPISSV
                     FPGMGNQILIICFVVVVIGGIGSVNGAMIASLAIGLADTLGKVIAAEYSAIAVYLVMA
                     LILLWRPQGIANRA"
     gene            complement(1610..2749)
                     /locus_tag="VFY96_13975"
     CDS             complement(1610..2749)
                     /locus_tag="VFY96_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607627.1"
                     /translation="MIGLGPISLQETAVAQTQKIRVGLMLPYTGTYAQLGTAITNGFK
                     LAVAEKGGRLGGRELEYFTVDDEAEPAKAPENTNKLVQRDKVDVLVGTVHSGVQMGMV
                     KIARENNTLLIIPNAGLDAATGGLCAPNIFRTSFSNWQPSFPMGKVLADRGIRSVISF
                     AWKYGAGEEAVAGFTEGFIKAGGKSVRGLWIPFPNVEFQALLTEIASIKPDAVFVFFA
                     GGGAAKLIRDYNAAGLRKTIPLFGSGFLTDGVLEATEEPAQGLETTLHYADGLNTKRD
                     REFRSAYSKAYTFAPDVYAVQGYDAALLLASGLEAVKGDITKRKEIVAAMEKAKIDSP
                     RGAWTMSKAHNPVQDIYLRKVVGRENKFVSVAHKALADPARGCKM"
     gene            complement(2915..>3272)
                     /locus_tag="VFY96_13980"
     CDS             complement(2915..>3272)
                     /locus_tag="VFY96_13980"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX5607628.1"
                     /translation="TVLEQGFRFLPIDGVVLKTIKALGYRPATMTKTRFNGLTEDLPT
                     IDFSGWPMIVHADMPTKVAYALCEAIEKRRDLIPTDNYKALDIAQLCANDEEAPYDAP
                     LHPGARRFYRERGYLK"
BASE COUNT          812 a          875 c          921 g          664 t
ORIGIN      
        1 ttgtaaccga agattggaaa accgatggag cgcatgcgct gctcgttaag acgaataccg
       61 gccagggcgc gaccaaacgg agcgtgcaat attcgtttga gcaatccata aacgaggatc
      121 aaaagaatca gcacgaaata atagaactgc agcgggcttt ccagatcgag aggcttgaat
      181 ccggcgatgg tcagctccgg tcgcacgtag atatagaggc catcggagcc gccgcctaac
      241 cgggtatcgt gaaaaaccga gtaaagcatc tgcgcgaacg cgagggttac catgatgaag
      301 taaaccccgg tggtgcgcag cacaaaaaac ccgatgacca aagcagcaaa ggcagcgata
      361 cccatcgcca acggcaaggt cagccataca ttggccgcct catacttggg cgagatcagc
      421 gccagtgcgt aggctgccaa gccgaagtac gcagcgtggc caaagctgac caaaccggtg
      481 tggccgacca gcaggtcgag gcttagcgca aaaatcgcca tgatcaaaat tttggcgccg
      541 agctggatat agaacgtcga gcccgtgagc ggaaacagcg cgagcaacac caaagcgatg
      601 aacagcagtg aacgcggcag agtgatcggg atcacgggcg tcacgctcta ttggcaatgc
      661 cctgagggcg ccacaggaga atcaacgcca tcaccagata aaccgcgatt gccgagtatt
      721 ccgcggcgat caccttgccg agggtgtcgg cgagaccgat ggccaaggac gcgatcatcg
      781 cgccgttgac cgagccgatg ccgccaatca cgacgacaac gaagcaaatg atcaggattt
      841 gattacccat accagggaag accgacgaaa tcggggcagc aacggcgccg gcgaaacccg
      901 ccagagccgt accggcggca aagacccacg cgaacagcag gggaacgttg acgcctaagg
      961 aggctgcgat ttcgcggtta ctgttgccgg cgcgaatcat catgccaacg cgggttttgt
     1021 aaatcaccca ataaagcagc gcggcggtaa tcaggcacac gccagtaacg aacaggcgat
     1081 aaaccggata ggacaaagta tcggtgagag gaatggagta gttgaggcca gcgggaattg
     1141 tcacgccatg gacgtcgttg ccgaataaga tactgcgcaa ttcttcaaag atgaggatca
     1201 agccgaaagt actcaatact tgatagagat gatcgcgccg gtaaagccgc cggatgatcg
     1261 tggtctccaa cagaccgcca atccccagag tgacgacggt gccgatcgga atcgccaacc
     1321 aaaaattgcc agtgagtttg gcgagccaat aggcgaggta ggcgccgacc atataaaaac
     1381 tgccgtgagc gaggttgatg acgcccatga tgccgaagat caacgtgagg ccgctcgata
     1441 acaagaacag taggaggccg tactggactg cgtttaataa ttggatcaaa tagaacatgt
     1501 tcaaaaggtt caatcagttc aaaacgttca ggccgtttaa ctggaatggg ttcaaagatg
     1561 attcgtacga ttcgaacgct ttgaacgatt ggaacacagc gtcggggcgc tacatcttgc
     1621 atccgcgcgc gggatcggcg agtgccttgt gggcgacgct cacaaacttg ttttcacgcc
     1681 cgacgacttt tcggagatag atgtcctgga ccggattgtg tgccttggac attgtccagg
     1741 cgccgcgcgg gctatcgatc tttgcttttt ccatagccgc gacgatctct tttcgcttgg
     1801 tgatgtcgcc tttgacggcc tctagtccgg aagcaagcag cagagcggca tcgtagcctt
     1861 ggaccgcgta tacgtcgggc gcaaaagtat aggccttaga gtaggcgcta cgaaactcgc
     1921 gatcgcgttt tgtattcaag ccatcggcgt agtggagcgt tgtttccagc ccctgagccg
     1981 gttcttctgt cgcttcgagc acaccgtcgg taagaaagcc ggagccgaat aatggaatgg
     2041 ttttcctcaa tccggccgca ttatagtctc tgattaactt ggcggccccg ccgccggcaa
     2101 aaaacacaaa caccgcgtcg ggcttgatgg aagcgatctc cgtgagcaac gcctggaact
     2161 cgacgttagg aaacggaatc cagagaccgc ggaccgattt cccgccggct ttgatgaatc
     2221 cttcggtaaa gccggctact gcctcttcgc ctgcaccgta cttccaggca aaagagatga
     2281 cgctgcggat gccgcggtct gcgagcacct tgcccatggg gaaagaaggc tgccaattgg
     2341 aaaacgaggt gcgaaagata ttcggcgcgc agagacctcc ggtggcggcg tcgagtccgg
     2401 cattgggaat aatcagcagc gtgttgtttt ctctggcgat cttgaccatg cccatttgca
     2461 cgccgctgtg cactgtgccc accagcacgt cgactttgtc gcgctggacc agcttattgg
     2521 tattctccgg cgccttggcg ggctcggctt catcgtcgac ggtgaaatat tccaattctc
     2581 ggccccccag tcttccgcct ttctcggcga cggccaattt gaagccgttg gtaatcgcgg
     2641 ttccaagttg agcgtaagtt cccgtatagg gaagcatcaa acccacgcga attttttgtg
     2701 tctgcgcgac cgcggtttct tgaagtgaaa tcgggccaag gccgatcaaa ccggcaaata
     2761 acagcaactt tctaaaattg atgttgcgat caactttcac ttgcgcctcc tgtcgtcgac
     2821 atggtgaaac ttgactatct tgcggttcgt catagccacg ccgcgctatt tttactatac
     2881 aaacgaaatt ttcggcaagc ttgctcagaa gtgtttactt caaatagccg cgctcacgat
     2941 aaaagcgccg agccccagga tgaagcggcg catcgtaagg cgcttcttcg tcattcgcgc
     3001 agagctgcgc gatatcgagg gctttataat tgtccgtggg aattagatcc cgccgttttt
     3061 ctatcgcttc acagagcgcg tacgccactt tagttggcat atccgcgtga acgatcatcg
     3121 gccagccgct gaagtcaatc gtcggcagat cctcggttaa cccgttgaaa cgcgttttcg
     3181 tcatcgttgc cggccgatag ccgagcgctt tgatggtttt gagaacgacg ccgtcgatcg
     3241 ggagaaagcg aaagccctgc tccaagactg tt
//
LOCUS       DASULZ010000674         4615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_483103, whole genome shotgun sequence.
ACCESSION   DASULZ010000674 DASULZ010000000
VERSION     DASULZ010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483103"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VFY96_13985"
     CDS             complement(<1..463)
                     /locus_tag="VFY96_13985"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5607629.1"
                     /translation="MSPNQILQGVRILAFTTGYAGPYAGRLLAAYGAEVIKIESRKGG
                     LDTFRHYGQHKDIDAAPRFIECNLGVRSLAVNLKHPTGVRLIRELAGKSDAVLQNFRP
                     RVLDKLGLRDDELRKSNPKLVVVKLPGFGSEGPKSSYGTWGFNLTAFSGITY"
     gene            complement(468..1655)
                     /locus_tag="VFY96_13990"
     CDS             complement(468..1655)
                     /locus_tag="VFY96_13990"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5607630.1"
                     /translation="MTNNKQPTTLLSGYRVLDLSSSMGAFCGKMLRDLGMDVVKVEPP
                     GGDSARWEPPFAKGREHREGSLRFAYLNAGKRSVALDITQEFGRKAFLELVGRSDVVL
                     ESFEPGYLEAHDLGYHDLIEKQKKLILASVSGFGQDGPYAHFKTPDIVGNAMGGLLYI
                     SGDPTMKPCNPPETQSYYYASLFACYGVMLALWQREMRGIGAWIDISIEASMALHEHV
                     AFNYSAEGRVMKRAGSQHQHNAPANLFQCKNGWIALFVTQNHWPLLLRIWDDHDPALD
                     DPKWINSNLRRQHADYINAQVTSFTSRFFKEELAELFQKNGIPGLPVNSPSDFMKDPH
                     ILARGFFGTVTHPILGTFLQPGNCFMVDGARGGPMPAPLLGQHNDEILRGELGLNVDE
                     VVD"
     gene            complement(1738..2901)
                     /locus_tag="VFY96_13995"
     CDS             complement(1738..2901)
                     /locus_tag="VFY96_13995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5607631.1"
                     /translation="MDFDLPEELKLLKDTVRKFVDRELIPLERECRPEGEDMPEQYIK
                     PLQEKAKAIGLWLLDVPQEYGGAGLDLMSRCVIMEEVARTVAIPFRYAPIFGPEVRPV
                     LFHCDEEQKKRFLFPVIRGEKRICFAQTEPDAGSDPAGMKTRAVRDGDYYVINGTKRF
                     ITGAKNADYAQVIAITDPQKGARGGVSCFIVDMKAPGVTLERLWPTMMGDTPGQIHFE
                     NVRVPAADMIGREGEGFSIAQGWIQEGRIRGHGARPVGIASRALDMMIEYSKVRTTFG
                     RPLADRQAIQFMIADSAMELHACRLMVYECAWRHDRGEDVRQLSYMTKIQCAEMASRV
                     VDRSIQVHGGLGLMRELPLEWWYRQVRSIRITEGTPEVLRWRLAQHIIKNHKG"
     gene            complement(3016..3855)
                     /locus_tag="VFY96_14000"
     CDS             complement(3016..3855)
                     /locus_tag="VFY96_14000"
                     /EC_number="4.1.2.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxycinnamoyl CoA hydratase/lyase"
                     /protein_id="HEX5607632.1"
                     /translation="MAATLWKEFKTVKVEKENGITWCILNRPEKRNAMNPQLHYDMLD
                     ALTELEVDKETQVLVLTGAGPSWCAGQDLREYFRGTDNNPLERRKASWASQEWRWRRL
                     FYFPKPTIAMVNGFCFGGAFTPLIACDFAIAAEDATFGLSEVNWGIFPGGLVSRVLAD
                     AMSYRDAMWYIMTGDPFDGKKAAEMKLINYAVPKEKLREETVSLAQKLMKKNPQVLRA
                     AKEVYKYCRTMDYAQAEDYMSAKGTALRLTDPEKGRETGMEQFLDKKTYRPGLGEYSR
                     SAK"
     gene            complement(4086..>4615)
                     /locus_tag="VFY96_14005"
     CDS             complement(4086..>4615)
                     /locus_tag="VFY96_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607633.1"
                     /translation="QVLQLGSSPNMLASLDKGGVEAAVLTIPSMFVAEDRGYRVLLDM
                     ADTDIYYLHTMVATTRQYIKNNRDKVLRFLRGYVEGIAFVKHNKRESLDVVKKKLRLG
                     PDQERNLERSLDLLIAKYYESVPYPSLRGVETVLGFIEKDNPKAKSADPKSFVDDSLL
                     REIEQSGLIKKLYDR"
BASE COUNT          988 a         1337 c         1231 g         1059 t
ORIGIN      
        1 aatacgtgat cccggaaaaa gcggtcaggt tgaatcccca agtgccgtag ctgctcttcg
       61 gtccttcgct gccgaaaccg ggaagcttca cgacgacgag tttaggattg gattttctga
      121 gctcatcatc cctcaaaccc aatttatcca ggactcgcgg acgaaaattt tgcagtacgg
      181 catcggattt gccggcgagc tcgcgaatca gccgcacacc ggttggatgt tttaggttca
      241 cggcaagaga acgcacgccg agattgcatt cgataaaacg cggcgccgca tcgatatcct
      301 tgtgctgacc gtagtgccga aaggtatcta agccgccctt ccgagactcg atcttgatga
      361 cctccgctcc gtaggcggca agcaaacgcc ctgcgtacgg cccagcgtag ccggtcgtga
      421 aagcgagaat acgcacgcct tgaagaatct ggttgggcga catggaatta atcaaccacc
      481 tcgtcgacat tcagccccaa ctcgccgcgc agtatttcat cattgtgttg tcctaacagg
      541 ggcgcgggca ttggcccccc gcgcgcgcca tcgaccatga agcaatttcc cggttgcaga
      601 aaggttccga gaatcggatg cgtgacggtg ccgaagaacc cgcgcgctaa aatatgcggg
      661 tctttcatga aatccgacgg tgaattcacc ggcaacccag ggatgccgtt tttctggaac
      721 agctcggcca attcttcttt gaaaaaacgc gacgtgaatg aagtcacttg ggcgttgata
      781 tagtctgcat gctgtcggcg caggttgctg ttgatccatt ttggatcatc gagcgccggg
      841 tcgtgatcgt cccagattcg cagcagcaac ggccagtggt tctgggtaac gaatagtgcg
      901 atccagccgt ttttgcactg gaacaaattc gccggcgcgt tgtgctggtg ctggctaccg
      961 gcccgtttca tcacccgtcc ttcggcggaa tagttgaacg ccacgtgctc gtggagcgcc
     1021 atgctcgcct cgatggatat atcgatccat gcgccgatgc cgcgcatctc gcgctgccag
     1081 agcgcgagca tgacaccata acaggcgaag aggctggcat aatagtacga ttgagtctcc
     1141 ggcggattgc acggtttcat ggtcggatcg ccgctgatgt agagcaagcc gcccatggca
     1201 ttaccgacga tatccggtgt tttaaagtgc gcgtagggtc cgtcttggcc gaagccactc
     1261 accgaggcca ggatcagttt cttctgcttt tcgatcagat cgtggtagcc gagatcgtgc
     1321 gcctcgagat atcccggttc gaagctttcg agcacgacat ccgatcgccc caccaactca
     1381 agaaatgcct ttcgtccgaa ctcctgcgtg atatccagcg ctacgctgcg cttgccagca
     1441 ttgagatagg caaagcgcaa gctgccttcg cgatgctctc ggcctttggc gaagggcggc
     1501 tcccatcgcg ccgaatcgcc gcccggcggc tcgactttaa ccacatccat gcccagatct
     1561 ctgagcatct tgccgcaaaa cgcgcccatg gaagagctca gatcgagcac gcgataaccg
     1621 gacaggagtg tcgtcggttg tttgttgttg gtcattgatg cccgttgctc ttaggcgaga
     1681 atcgaagatg gaggatggaa aatggcttga acaccatcca cgatcctcaa ccagatttca
     1741 tcctttgtga tttttgatga tgtgctgcgc caagcgccag cgcagcacct cgggcgtgcc
     1801 ctcggtgatg cgaatgcttc gtacctgtcg ataccaccat tccagcggca gctcgcgcat
     1861 taatcctaaa ccgccgtgca cttgaatcga gcggtcaacg acgcgactcg ccatctccgc
     1921 gcattggatt ttcgtcatgt aagagagctg acgaacgtct tcaccgcggt cgtggcgcca
     1981 ggcgcattcg taaaccatca atcggcaggc gtgcaactcc atagcggaat ctgcgatcat
     2041 gaattgaatc gcctgccggt cggccaaggg ccggccgaaa gtggtgcgca ctttggagta
     2101 ctcgatcatc atgtcgagag cgcgcgaggc gatgcctacc gggcgggcgc cgtggccacg
     2161 gatgcgcccc tcttgaatcc agccctgtgc gatcgaaaaa ccctcgcctt ccctgccgat
     2221 catgtccgct gccgggaccc gcacgttctc gaaatggatc tgccccggcg tgtcgcccat
     2281 catcgtcggc caaagccgct cgagcgtcac accgggcgct ttcatatcga cgatgaagca
     2341 gctgacgccg ccgcgggcgc ccttttgtgg atctgtgatc gcgatgactt gcgcataatc
     2401 ggcatttttg gcgcccgtaa tgaaccgttt ggtgccgttg atcacatagt aatcgccgtc
     2461 tcgcacggcg cgggtcttca tgcctgctgg atcactgccg gcgtctggct ccgtctgagc
     2521 gaagcaaatc cttttttcgc cgcgaatcac aggaaacaga aaacgctttt tttgttcctc
     2581 atcgcaatga aacagaaccg gccgtacctc cgggccgaaa atcggcgcat agcgaaaggg
     2641 aatcgccacc gtgcgcgcga cttcttccat gatcacgcag cggctcatca ggtcgaggcc
     2701 ggcaccgcca tattcttgcg gtacatcgag cagccacaaa ccaattgcct tggctttttc
     2761 ctgcagcggc ttgatgtact gctcgggcat gtcttcgccc tcgggtcggc attcgcgctc
     2821 gagcgggatc agctcgcgat cgacaaactt gcgcaccgtg tcttttagaa gtttaagctc
     2881 ttcggggaga tcaaaatcca cgattgcacc agatattcat cgctgaaagc tcgacagcgc
     2941 ctacaatccc ccttctttcg cctttgataa gggggaaaga gggggatttg tcacgcggct
     3001 gactgcacta acaaactatt ttgcgctgcg actatactcg ccgagcccgg gccgataggt
     3061 ctttttatca aggaactgtt ccatcccggt ctcgcggcct ttctccggat cggtgagacg
     3121 aagcgcggtg cctttagcgc tcatgtaatc ttccgcctga gcgtaatcca tcgtgcggca
     3181 atatttgtag acttccttgg ccgcgcgcag cacttgcggg tttttcttca tcaacttctg
     3241 cgccagacta accgtctcct cgcggagctt ttctttcggc accgcatagt tgatcagctt
     3301 catttccgcg gctttcttac catcgaaggg atcgccggtc atgatgtacc acatggcgtc
     3361 ccggtaagac atcgcgtccg cgagcacgcg actgaccaag ccgccaggga aaatacccca
     3421 gttaacctcg ctcaggccaa aggtcgcatc ctcagccgcg atggcaaaat cacaagcaat
     3481 caaaggcgtg aacgcgccgc cgaaacaaaa gccattgacc atcgcgatgg tcggtttggg
     3541 gaagtaaaaa agccgccgcc aacgccattc ttgagacgcc cagctcgctt tgcgccgttc
     3601 cagtggattg ttatccgtgc cgcgaaaata ttcgcgcaga tcctgtccgg cgcaccaact
     3661 cggccccgca cccgtgagaa cgagcacctg cgtttccttg tcgacctcca gttccgtcaa
     3721 cgcatcgagc atgtcgtagt gaagctgcgg gttcatcgca tttctttttt ccggccgatt
     3781 gagaatacac caggtgatgc cgttttcttt ttcgactttg accgtcttga attctttcca
     3841 taacgttgct gccacgtttc gatacctcct cagagaattt gcgctgagct tatgcagccg
     3901 aagaagcggt tgtcaagcaa accattgtcg atccaaaaaa gtagccgcga aataaacgag
     3961 aagcttaagc tggcgcttgt tgctcacgtt caaaatcgac tcaacgacgt taactcagca
     4021 gagcgaggac gatgacccca aagctagttc atttcgagtc agagcggtgg gctgatgggc
     4081 cgcgattatc ggtcatagag ctttttaatc aatccactct gctcgatctc gcgcagcaga
     4141 ctatcgtcga cgaatgattt cgggtcggcg cttttagctt tgggattgtc cttctcgatg
     4201 aagccgagca cagtttctac gccacgcagc gatggatagg gcaccgactc gtagtacttg
     4261 gcgatgagta agtcgaggga gcgttcgagg tttcgttctt gatcggggcc gagccgtagt
     4321 tttttcttga ccacatccag gctttctctt ttgttgtgtt tcacgaaagc gatcccttcg
     4381 acgtagccgc gcaaaaaccg caaaacttta tcgcggttat tcttaatata ctggcgcgtg
     4441 gtggccacca tggtgtgaag atagtaaata tcggtatcgg ccatatcgag aagcactcga
     4501 taaccgcgat cttccgccac gaacatggaa ggaatcgtca ataccgccgc ctcaacgccg
     4561 cccttatcga ggctcgccag catgttcggc gatgaaccga gttgcagcac ctgaa
//
LOCUS       DASULZ010000675         2600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_787043, whole genome shotgun sequence.
ACCESSION   DASULZ010000675 DASULZ010000000
VERSION     DASULZ010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787043"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VFY96_14010"
     CDS             complement(<1..256)
                     /locus_tag="VFY96_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX5607634.1"
                     /translation="MIQLLLNIVVFISGAVLMALEIVGSRVLAPDFGSSIFVWGSLIS
                     VVMAALSIGYYWGGWLSAREPSYGKLLILLLIPGVMIFFLP"
     gene            complement(260..2260)
                     /locus_tag="VFY96_14015"
     CDS             complement(260..2260)
                     /locus_tag="VFY96_14015"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEX5607635.1"
                     /translation="MTTTYILKNFIPADRNTSGLDYRKELNDAQYEAATSMEGPLLII
                     AGAGTGKTRTLVYRVAHLIDQGVDPRSILLLTFTRRAAEEMLRRASLLIDGRCKQVAG
                     GTFHSFANLVLRMSGRHLGLAPSFTIMDRSDSEDVIQLIRTEMGLNTKEKRFPRKQTV
                     AEIFSMTLNKQTRLSDLLELEYPHLVELDYELGELLRRYTDYKRTKSLLDYDDLLTLL
                     RDLLADHAAVRNRLSDTYRFIMVDEYQDTNHLQAEIVRLLAATHQNVAVVGDDAQSIY
                     SFRGANFRNIMDFPKHFPGTRIIKLEENYRSTQPILNVTNEIIQRATEGYEKRLFTRK
                     SSGMRPKLVQAGSEQMQSQFVCQKILELREEGVPLWDIAVLFRSSFHSFDLEIELARH
                     SIPFVKRGGFQFMETAHVKDLLAHLRVLANPQDTISWQRVLMLLEGIGPGASQKIIKW
                     LLDGSSAVERLRSYPAKSKIAQGLRTLAQVLEEAGHAELPSEQSQYLLQYYIPILKQN
                     YPDDHPKRLRDMEHFQGMTERYRNLERLLSDMALEPPNDSIGGVLAVDPDEGPLILST
                     IHSAKGLEFHTVFIIWALEGRFPSFYNINSQEELEEERRLLYVAATRAKQNLFISYPT
                     RVFDKTLRMVLSQPSQFIDGIPQRLLEPVALVPQGSYWDWEN"
BASE COUNT          616 a          672 c          714 g          598 t
ORIGIN      
        1 aaggcaggaa aaaaatcatg accccgggaa tcagcagaag gatgagaagc ttgccatagg
       61 acggttcgcg cgctgagagc cagccgcccc aataatagcc gatgctcaaa gcagccatga
      121 cgactgaaat cagactgccc cagacgaaaa tggaactgcc gaaatcgggc gccaatacgc
      181 ggctgccgac gatttccaaa gccattaaaa ccgcaccgct gatgaaaacg acgatgttga
      241 gcagaagttg aatcatcaat caattctccc aatcccagta ggagccctgc ggtacgagtg
      301 ccaccggctc gagcagtctt tgcggtatgc cgtcgatgaa ctgtgatggt tgcgatagca
      361 ccatgcgcag ggtcttatcg aagacacggg tcggatagct gataaaaagg ttttgtttgg
      421 cgcgagttgc agcgacgtag agcagccggc gctcttcttc aagctcttcc tgcgagttga
      481 tgttgtagaa ggagggaaac ctaccttcca gcgcccaaat aataaatacc gtgtgaaact
      541 ccaagccttt tgccgaatga atggttgaaa ggatcagcgg gccttcatcc ggatcgaccg
      601 ccagaacccc cccgatgctg tcattgggcg gctcgagcgc catatcgctc agcagccgct
      661 cgaggtttcg gtagcgctcg gtcatacctt gaaaatgctc catgtcacgc agccgcttgg
      721 ggtgatcgtc gggataattc tgtttcagga tcggaatgta atattgcaac agatactggg
      781 attgctccga tggtaattcc gcgtgccctg cctcttccaa gacttgcgcg agggttcgta
      841 aaccttgagc gattttgctt ttggccggat acgaacgcag tcgctcgacc gcactgctgc
      901 cgtcgagcaa ccatttgata attttttggc ttgcacccgg accgattccc tccagcagca
      961 tcagcacgcg ctgccaggaa attgtatcct gcgggttggc gagaacgcgg agatgagcga
     1021 gtaaatcttt tacatgggcg gtttccatga attgaaaacc gccgcgctta acgaatggaa
     1081 tactatggcg tgcaagttcg atctccaggt cgaatgaatg gaagctcgac cggaacagta
     1141 cggcgatatc ccagagcggc acaccttcct cacgtagctc gagtattttt tgacaaacga
     1201 actgcgactg catctgctca cttcccgcct ggacgagctt gggccgcatg ccggaacttt
     1261 tccgcgtaaa aagccgcttt tcatagcctt ccgtggcgcg ttgaatgatc tcgtttgtga
     1321 cattcaaaat cggttgcgtg ctacggtaat tctcttcgag tttgataatc cgtgttccgg
     1381 ggaagtgctt cggaaaatcc atgatgttgc ggaaattggc gccgcggaag gagtaaatgc
     1441 tctgcgcatc atcgccgacc actgccacgt tttgatgtgt agcggcgagc aggcgcacaa
     1501 tctcggcctg gagatggttg gtatcctgat attcgtccac catgatgaag cggtacgtat
     1561 ccgatagccg gtttcgtacc gcggcatggt cggccaacaa atctcgcaac aaggtcaaaa
     1621 ggtcatcgta atcgagcagg gacttggtgc gcttatagtc ggtatagcgg cgcaatagct
     1681 cgccgagctc atagtccagt tcgaccaggt ggggatattc cagttcgagt aaatcgctca
     1741 gccgagtttg cttgttcagg gtcatgctga agatttcggc gaccgtttgt ttacgtggaa
     1801 aacgtttttc tttggtgttc aggcccattt cggtgcgaat cagctgaatg acgtcttcgc
     1861 tatccgagcg atccatgatg gtaaaggagg gcgcgaggcc gagatggcgg ccggacatgc
     1921 gcaacaccag gtttgcaaaa gaatgaaatg taccgcccgc cacctgtttg cagcgtccgt
     1981 cgatcaacag actggcgcgt cgaagcatct cctcggctgc gcgacgggtg aaggtcagca
     2041 gcaatatcga ccgcggatca accccctggt cgatcaggtg agcgactcga taaaccaggg
     2101 tccgggtctt accggtgccg gcgccggcga tgatgagaag cggcccctcc atgctcgttg
     2161 ccgcctcgta ctgtgcgtcg tttaattctt ttcggtagtc tagcccggag gtattgcgat
     2221 cggccggtat gaaatttttc aaaatataag ttgtcgtcaa gaaagctcca attgcccggg
     2281 taaattaccc gttttcctct gatccagcaa gcgtttgaac gagctaaagc tccatagttt
     2341 ggaatgacag aacggttttt gaagagggtt cacagcgtca tggcgatgcg acaaagcaaa
     2401 tcgacgggag ccgacccaat gggatttctt catcaagaaa agcccggacc ggagcgtagc
     2461 aatacgagta cttttcacct tacgcacgta gggtttgtca tacaacgcgg cgaccacgat
     2521 cgaaagccat cgcctttata tcggccgttt atcagggacc atttgttggt acaggccttg
     2581 cgctgcccca caagaagatc
//
LOCUS       DASULZ010000676         4336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_280395, whole genome shotgun sequence.
ACCESSION   DASULZ010000676 DASULZ010000000
VERSION     DASULZ010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280395"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VFY96_14020"
     CDS             complement(<1..321)
                     /locus_tag="VFY96_14020"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEX5607636.1"
                     /translation="MIFGRHSVLKRGCSTWDPKEVPQAEFQARLDRVRSEMAQQNLDA
                     LVTYGDNYSFADLCYLTNYFPKVRGGIAVVPRDGEISLLLNIGSRDVPFAKTLTWVED
                     VRASN"
     gene            complement(390..1499)
                     /locus_tag="VFY96_14025"
     CDS             complement(390..1499)
                     /locus_tag="VFY96_14025"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX5607637.1"
                     /translation="MDAREEFDRRLSRVRQSMLDKGLQTLLVYEGGRHNFLRMNYVAY
                     LTDFISVGPETMLLVPLEDAPVLYLSPVWDIPRAQEESFVREVRAFKEFWSRLAGLSG
                     NVGLIGREAMSVGLHDQITKTLGRTAVNAKDIIENMARSKTPLELERLRRAAEIADAG
                     VKALHEDARPGLREYELAAIVEYRMRSLGAEDNFGMVVANNHNQALHPPTDRVTQKGD
                     IIIGEITPCVGGLFVQICRTMVLGEPAPVVREKYAILKKAMGLGMEAATTGAPASAVA
                     AAINDTFIEYGYEQYCRPPFMRVRGHGLGCGSFAPGSLEDSNPTRLEEGMTYIIHPNQ
                     YLPETGYLTLGDTVVMTKTKAESLMKSSWDIFIKE"
     gene            complement(1721..3382)
                     /locus_tag="VFY96_14030"
     CDS             complement(1721..3382)
                     /locus_tag="VFY96_14030"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX5607638.1"
                     /translation="MSHPPVNPITVEVVRNGLAHIANEMATVLRKTSYNMMIYEVRDY
                     CVGIVDPDGNILSQNFGALPIFLADLGPAIVDGVRTHGRGGFQPGDVLIMNHPYICGQ
                     HLNNVVVYTPFFHERELVAFLAVRAHWIDIGGTRVGFGFSGTREIYEEGLQFRSLKLY
                     RGVEPNQDIFQIITDNVRFAESCLGDLRAQIAACRVGDRGLAALVTRYGREVFSQCLE
                     TIWRQSELLARKKVARIKPGRYEAEALFDSDGVDLEKPVSLKVVVEVAGDEMTIDFSE
                     ISEQVAGSINSGESGAVAAARVAFKSLVAPDSPIDEGCFRPLKIVIPPGKILSAEPPA
                     PVGNWSRTLPTVIDLILRALAPAMPDRVAAGHKGDMGGYAFFGINPNNGRRFLCQTIM
                     GGGWGGRAHEDGENATVSMCQGDVQNAPVELQELYYPVLIERQQLREDSGGAGKFRGG
                     LGIEIAVRVLCDAFTNINIERQRTAPWGIFGGACGAKAKALVKQAADDPGSWLTKKPN
                     YPLKKDGSVIFFTAGGGGYGPPSERAPELIERDRRLGYVSAANGP"
     gene            complement(3500..4282)
                     /locus_tag="VFY96_14035"
     CDS             complement(3500..4282)
                     /locus_tag="VFY96_14035"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX5607639.1"
                     /translation="MTVDQSTYETLLKLVKVRQSVRKFRPDPIPEDTINKILEVGRWA
                     MSGANSQPWEFVVVTDAEIKKQLRDAYSEYNTDFIFWMEQQREYNLRHPSYQVKNDPH
                     ESLRFNKAKANWHQAPAVIVVLGDGRRQWGTVMGAHTFGRDQSHLTDSLANASFLIHL
                     AVASLGLTSEWVSIHIEEPHKRILGVPDLLKLYLIIPIGYPDVPAHEGVRRPLEEFVH
                     RERYDMAKYMSNEDVVKYLYSLREGTVSGYSASRAVSTDDKK"
BASE COUNT          921 a         1301 c         1185 g          929 t
ORIGIN      
        1 gttcgatgcg cgaacgtcct cgacccaggt cagcgtcttg gcaaacggca cgtctcggct
       61 accaatgttt agcagcaacg aaatctcgcc gtcgcgcggg acgacggcga tgccgccccg
      121 caccttggga aaataattcg tcaaatagca aaggtcagcg aacgaataat tgtcgccata
      181 ggtcaccagc gcgtccaagt tttgctgtgc catttcactg cgcacgcgat cgagacgcgc
      241 ttggaactca gcctgcggca cttccttggg atcccaggtc gagcagcctc gcttcagaac
      301 cgaatgtcgt ccaaaaatca tctctacctc atggcgccgg ctgatccggc ggcttgaacg
      361 agttgaacgg atcgaacgat ttgaactctt tactctttga taaagatatc ccaggacgat
      421 ttcatcagcg attcggcctt ggttttggtc attaccacgg tgtcgccaag cgtcagatag
      481 ccggtctcgg gtaaatactg gttggggtga atgatatagg tcatgccttc ttcgaggcgt
      541 gttgggttgg agtcttccag gcttcccggc gcaaacgagc cgcagccaag gccgtggccg
      601 cgcacgcgca tgaacggtgg ccggcagtat tgctcatagc cgtactcgat aaaggtgtcg
      661 ttgatcgccg ctgccaccgc gctcgccggc gcaccggttg tcgcggcttc catgcccaag
      721 cccatagctt ttttaaggat ggcgtatttc tcgcgcacga ccggcgccgg ctcaccgaga
      781 accatcgtgc ggcaaatctg cacgaacaaa ccaccgacgc agggcgtaat ctcaccgata
      841 atgatatccc ctttttgcgt gacgcgatct gtaggtggat ggagcgcctg attgtgattg
      901 ttagcgacga ccatgccaaa gttgtcttca gcgccgagcg agcgcatccg atattcgacg
      961 atcgcggcaa gctcgtactc tctcaaaccc ggacgcgcat cttcgtgtag cgctttgacc
     1021 cccgcatcgg cgatttcggc cgcgcgccgg agccgctcta gctcgagcgg cgtcttcgaa
     1081 cgcgccatgt tctcgatgat gtctttggcg ttgacagccg ttcgaccgag cgttttcgta
     1141 atctggtcgt gtaagccgac cgacatcgct tcccgtccga tcagtcccac gttgccggag
     1201 agcccggcca aacgcgacca gaattcctta aatgctcgca cctctcggac aaaagattct
     1261 tcctgcgcgc gcgggatgtc ccacaccggc gagaggtaga ggaccggcgc atcttctaga
     1321 gggacgagca gcatagtctc cggcccgacg ctgatgaagt cggtcagata agccacgtaa
     1381 ttcatgcgca gaaaattgtg ccgcccgcct tcgtaaacga gcagcgtctg caatcctttg
     1441 tccagcatcg attgccgcac gcgtgacaat cgccggtcga attcttctcg cgcatccatc
     1501 gtcacctcgc gttttcaaac gtcattcaag cacggctctg tggcagtcct cccgataacc
     1561 gcaaccaacg aattgtcatg ctgagcggag cgaagcatct cgcgtcacca tcacatgcga
     1621 caaagatcct tcgccgtggc ggctcaggga tgacacaatg acgtagcttc tctgcagaga
     1681 cgctaccgaa agctatagac ggcgctaact cggaaatttt ttatggtcca tttgctgccg
     1741 aaacgtagcc caatcttcta tcccgttcaa tcagctcggg cgcgcgctcg ctcggcggac
     1801 cgtaaccgcc gccgccggcc gtgaagaaaa ttacactgcc gtcctttttc aaggggtagt
     1861 tcggcttctt agtgagccag ctccccggat catcggctgc ctgcttcacc aacgcttttg
     1921 ctttcgcgcc gcacgcaccg ccgaaaattc cccacggcgc ggtacgctgc cgttcgatat
     1981 tgatgttggt gaaggcatcg cagagaacgc gcaccgcgat ctcaatcccc aagccgccgc
     2041 gaaatttccc ggcgccgccg ctgtcttctc gtagttgctg acgctcgatc agcaccgggt
     2101 aatacagttc ctgcagctcc accggcgcat tctgcacatc gccctgacac atcgatacgg
     2161 tcgcgttttc gccatcctcg tgggcgcgcc caccccaacc gccgcccata atcgtttgac
     2221 agagaaatcg cctgccgttg ttgggattga ttccgaagaa tgcataaccg cccatgtcac
     2281 ccttgtgacc ggcagcgact ctatcgggca tggcgggcgc caaagctcgg aggatcaaat
     2341 cgatcactgt cggtagtgtt cggctccagt ttcctacggg tgcgggcggc tcagcgctca
     2401 aaatttttcc cggcggaata acaatcttca acgggcgaaa gcatccttca tcgataggcg
     2461 agtcgggcgc gacgagcgat ttgaatgcaa cccgcgccgc cgcaaccgcg cccgattccc
     2521 cggagttgat cgaacccgcc acttgctcgc tgatctcgga gaagtcgatg gtcatctcgt
     2581 cgccggcgac ttcgacaacg accttgagcg acactggttt ttccaaatcg acgccgtcgc
     2641 tgtcgaacaa ggcttccgct tcgtatcggc cgggcttgat gcgggcgacc ttcttccggg
     2701 ccagcagctc ggattggcgc cagatcgttt ccagacattg cgaaaaaact tcgcgaccgt
     2761 accgagtgac cagcgcggcg aggccgcggt cgccaacacg acaggcggcg atctgcgcgc
     2821 gcagatcccc aaggcaagat tcggcgaagc gcacgttatc ggtgatgatt tgaaaaatat
     2881 cctgattggg ctcgacgccg cgatagagct tgagcgaacg aaactgcaaa ccttcttcgt
     2941 aaatttctcg cgtgccgctg aacccgaaac cgactctggt gccgccgata tcgatccaat
     3001 gagcgcgcac tgcaagaaac gccaccaact cgcgttcatg aaagaacggc gtataaacca
     3061 cgacgttgtt gagatgttgt ccgcatatat aggggtggtt catgatgagc acgtcaccgg
     3121 gctgaaaacc gccccggcca tgggtgcgca cgccatccac gatcgccggc cccaaatcgg
     3181 cgagaaaaat tggcagagcg ccgaagtttt gcgaaagaat attgccgtcc ggatcgacga
     3241 tgccgacgca gtaatcgcgc acctcgtaga tcatcatatt ataggaagtc ttgcgcagca
     3301 cggtggccat ctcattggcg atatgcgcca agccgttgcg cacgacctcg acggtgatcg
     3361 gattgacggg tgggtgggac atttcaatca gcggaaagaa atattaccac agaggcgcgg
     3421 aggacacaga gagttacgaa tgataatgct ctggtttctc tgtgcctctg cggtaagccg
     3481 cctgcgtttt cgcggagctt cattttttgt cgtcggtgct caccgcccgt gacgcggaat
     3541 agcccgacac cgtgccttca cgcagcgaat aaagatattt cacgacgtct tcattggaca
     3601 tgtacttcgc catgtcgtaa cgctcgcgat ggacaaattc ctccagcggc cggcgtacgc
     3661 cctcgtgcgc cggcacgtcg ggatatccga tggggataat cagatagagc ttcaacaagt
     3721 cgggcacgcc gaggatccgt ttgtgcggtt cttcgatgtg aatcgaaacc cactctgaag
     3781 tgagacccaa agatgccacc gcgaggtgga tcagaaacga agcgttggcg aggctatcgg
     3841 tcaagtgtga ctgatcacgc ccgaacgtgt gcgcgcccat gaccgtcccc cactggcgcc
     3901 ggccgtcgcc gagaaccacg atcaccgccg gagcttggtg ccaattggct ttggccttgt
     3961 tgaaacggag tgattcgtgc gggtcgttct tcacctggta cgacggatgg cgcaagttgt
     4021 actcgcgctg ctgttccatc cagaagataa aatcggtgtt gtactccgag taagcatcgc
     4081 gcaattgctt tttaatctcg gcatccgtca cgacgacaaa ttcccacggc tgcgaattgg
     4141 cgcccgacat cgcccagcgc ccaacctcga gaattttatt gatcgtgtcc tcgggaattg
     4201 gatcgggccg aaacttgcga acactctggc gcaccttcac cagcttcagc agcgtttcat
     4261 aggtggattg atcaacagtc attcgtttga ttcctccggg taggaccgat cctctagctc
     4321 acgagagacg ctggag
//
LOCUS       DASULZ010000677         2435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_380993, whole genome shotgun sequence.
ACCESSION   DASULZ010000677 DASULZ010000000
VERSION     DASULZ010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380993"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VFY96_14040"
     CDS             <1..684
                     /locus_tag="VFY96_14040"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607640.1"
                     /translation="VRIVAGFLDNWPMTLVAQPELKSLKDLKGKTLGISSFGATPDLG
                     ARMMIKQAGIDPDKEIKILALGSDAARLTALKQRVVDVVVISPPADTQMEKQGYRILA
                     RAYELFSFPYLGLGTHTRKIKEKPEEIRRVLKATIRANRFIRDNRDESVRTLIAWGKV
                     EREFAYASYDALRNLFNADGAVPEDGLKLVIDQARRSAKTTREITPGEVADLTFLHQA
                     QGDLGIKAR"
     gene            886..957
                     /locus_tag="VFY96_14045"
     CDS             886..957
                     /locus_tag="VFY96_14045"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607641.1"
                     /translation="MSNRSKTIYTGVTNNLERRVYPA"
     gene            1174..2106
                     /locus_tag="VFY96_14050"
     CDS             1174..2106
                     /locus_tag="VFY96_14050"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5607642.1"
                     /translation="MIIDWHAHVYPPELANERRWGGTTPLTIENLLEAHEKAGIDRCV
                     VSNTIHYLKEKTNDESLAFLRRWNEYAAEIQQKYQHRVIVFTSTLPCGGAPFVKELER
                     AIVDYGLHGVLINSSHQRAYPDDDEAKPFFEIVTSLKIPVMMHAPSVGFGEERMRDYR
                     LASSVGRPFDECLSIARMIVRGIFERHPDMKFVGCHLGGGICDVIGRMDYAYELGDLA
                     SGLGSYEPMLITKRPSEYLKKLYMDTVAYHPPAIMCAYETVGSKQLLFGSDAPPLLPL
                     LPRAKTIIEELPITDSEREDIFGRNALKLLQRTY"
     gene            2170..>2435
                     /locus_tag="VFY96_14055"
     CDS             2170..>2435
                     /locus_tag="VFY96_14055"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEX5607643.1"
                     /translation="MPLSYDDLIEPTIVRQISKSVAYCRCTRSKNLPFCDGSHAVTNI
                     QPFILELKEPETIAICRCWKSKDHPYCDGTHGRLVKPKERPPRQ"
BASE COUNT          636 a          667 c          625 g          507 t
ORIGIN      
        1 gtgagaatcg tcgccgggtt cctcgataac tggcccatga cattggtcgc acagcccgag
       61 cttaagtcgc tcaaggacct gaaagggaaa acgttaggca ttagcagctt cggggcgacg
      121 cccgatctcg gcgcgcgcat gatgatcaag caggccggca tcgatccgga taaagagatc
      181 aagattttgg cgttgggctc cgatgccgcc cggctcaccg ctctcaagca gcgggtcgtt
      241 gacgtggtgg tgatttcacc gccagcggac acgcaaatgg aaaagcaagg ctatcgcatt
      301 ctggcccgcg cctacgaact tttcagtttt ccctatctcg gtctcggcac ccacacgcga
      361 aagatcaaag agaaaccaga agagattcgg cgcgtcctca aagcaaccat ccgtgccaat
      421 cgtttcatcc gggacaaccg tgatgaatcc gtgcggacgc tcatcgcctg gggcaaagtc
      481 gagcgtgaat tcgcctacgc atcctatgac gcgcttagaa atctttttaa cgccgacggc
      541 gcagtccccg aagatggatt gaagctcgtc attgatcaag cgcggcgcag tgcgaaaacc
      601 acccgcgaga tcacgccggg cgaagttgcc gatctgacgt ttctacatca agcgcaaggg
      661 gacctgggca tcaaggcacg gtgacgttgt tgaaacaata agaaagaagg atcgcaactg
      721 ccaaggctaa taatgcacga aaatgctaca gcgctcgact gtcattctga ggagcgtgag
      781 cgacgaagaa tctgaagcac atagatcctt cgctaaaacg ctcaggatga catctgagca
      841 gtttgtattc tgtacatgcc atcgaaacac gactacgttt acattatgag caaccgctca
      901 aaaacgatct acactggtgt aacaaataat ctggagcggc gcgtataccc agcataaatc
      961 aaaacagatt cagggattca ccgcaaaata taacctcacg cgattacgaa caaactactg
     1021 atgttctctc ggcgatttcc cgagaaaaga ctatcaaagg ttggcttcga gctaaaaaag
     1081 tcattggttg aatcggtcaa tccggcttgg aaagatctta gcgcgatttt ctataatatc
     1141 gaataaagag aaatttgatt aggagttccc aatatgatca ttgactggca cgcccatgtc
     1201 tacccgccgg aacttgccaa tgagcggcgc tggggcggca cgacgccgct caccatcgaa
     1261 aatctccttg aagcccacga gaaggccggc attgatcgtt gcgttgtcag caacaccatt
     1321 cattatctta aagaaaaaac caacgacgaa tcgctggcgt ttctgcgccg ctggaacgag
     1381 tacgcggccg aaattcagca aaaatatcaa caccgagtga tcgttttcac cagcacgttg
     1441 ccctgcggcg gcgcgccatt tgtcaaagag ctcgaacgtg cgattgtcga ttacggcctg
     1501 catggagtgc tgatcaattc gagccatcag cgcgcttacc ccgatgacga cgaagcgaaa
     1561 ccgtttttcg agatcgtcac cagcctgaaa attcccgtca tgatgcatgc gccatcggtc
     1621 ggcttcggtg aagagcgcat gcgcgattac cggctggcct cgagcgtcgg ccgaccattc
     1681 gatgagtgtt tgagtattgc gcgcatgatc gtccgcggca tcttcgagcg ccacccggac
     1741 atgaaattcg tcggctgcca tctcggcggc ggaatctgcg acgtcatcgg ccgcatggat
     1801 tatgcctatg agctcggtga cctcgcatcc ggcttgggat cgtacgagcc catgctgatc
     1861 accaagcggc caagcgaata tttgaaaaaa ttgtacatgg acacggtcgc ctatcatccg
     1921 ccggcgatca tgtgcgccta tgaaacagtt ggcagcaaac agctactgtt cggcagcgac
     1981 gctcccccgc ttttaccact gctgccgcgc gccaagacaa ttatcgaaga gttgccgatc
     2041 acggattcgg aaagggaaga tatttttggc cgaaatgcgc ttaaattgct acagcgcaca
     2101 tactagcgct ggcgcagttg cgaaggggaa atcagttgta ataaattgaa tcgggaaagg
     2161 aatcgctaca tgcctctatc ctatgatgac ctgatcgaac cgaccatagt ccgacaaatt
     2221 tccaaaagtg tggcctactg ccgctgcacc cgaagcaaaa acttgccgtt ctgcgacggc
     2281 tcgcacgcgg tgaccaacat tcaaccattc attttggagc tcaaggagcc cgaaaccatc
     2341 gccatctgcc gctgctggaa atccaaagat cacccttatt gcgacggcac tcacggtcgg
     2401 ctagtcaagc cgaaggagcg tccgccacgc caaca
//
LOCUS       DASULZ010000678         2566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1091253, whole genome shotgun sequence.
ACCESSION   DASULZ010000678 DASULZ010000000
VERSION     DASULZ010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091253"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..826
                     /locus_tag="VFY96_14060"
     CDS             134..826
                     /locus_tag="VFY96_14060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX5607644.1"
                     /translation="MIDVAANYRKILGSIAEATAKRRHNAGQVKLLAATKSQSIELIL
                     AAVDAGITLIGENYVQEAAGKRPLITAAIEWHMIGHLQRNKVKAAVDLFDVFESLDNL
                     TLARELDKEGQKRKKMIRAFIEINLGGEEGKTGVVESEAASLVEQIATLSHLRIEGLM
                     SVPPYREDPEEVRPYFRKLRLIQERLGEICSPRLELRELSMGMSHDYKVAIEEGATIV
                     RIGTALFGPRRD"
     gene            830..1129
                     /locus_tag="VFY96_14065"
     CDS             830..1129
                     /locus_tag="VFY96_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX5607645.1"
                     /translation="MFVLANFLFATAQVLDYVLWAYLWILVARFVISLVNADYDNPIV
                     RFVYAATEPVLERVRERMPVFAAGWDLSPLVVWIAIIFLQRFLVRSLYDLAYSMR"
     gene            1308..>2566
                     /locus_tag="VFY96_14070"
     CDS             1308..>2566
                     /locus_tag="VFY96_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5607646.1"
                     /translation="MKIFLAGNWVDKPKKIEVRNPFDNSVFETVPHADAEDLEKALAY
                     AERGAKVMAKLSSYERWKILRKAADLIAARNEELGQIISKEEGKIIAEGRGEANRAVE
                     TMMGSAEEAKRLHGETVPLDADPTGGKKFGFTLRVPCGVVAAIAPFNFPLNLVAHKVG
                     PALAAGNSVIVKPASDTPLSALKLTEILLEAGLPAEGIQCITGSGGEIGDALVADRRV
                     RKVTFTGSREIGERICQRAGIKKVTMELGSNSPVIIMPDADLEKVATVLAMTGYGNAG
                     QTCISTQRVLTAKSIYGDLLSVMKPKVEALTTGNQLDEKSKVGPMVKESEAVRVDKWI
                     NEAVASGARLVAGGGRRGAIYTPAVVADVKPNMRISRDELFGPAVAVTPFNDIDEAIS
                     LANDSVYGLAAGIFTENVEWAMKFAREA"
BASE COUNT          616 a          590 c          778 g          582 t
ORIGIN      
        1 gccatgagcg ggaggtattc gaaagccatt ggcaatcacc ggttgcaaag acttctactt
       61 tactcgcttg acgcaagcaa gcccgcccag tcggagtgaa ttattcccgg tgctatggta
      121 agcagtgtct ctcatgatcg atgtcgctgc taattaccga aaaattctcg gctctatcgc
      181 cgaagcgacc gccaagcgcc ggcacaatgc ggggcaggtg aaattgcttg cagcgaccaa
      241 gtcacagagc atcgagttga ttctcgccgc agtggatgcg ggcatcactt tgatcgggga
      301 aaattacgtt caggaagctg ccggcaaaag gccgctgatc accgcagcga ttgaatggca
      361 catgatcgga catctgcaac ggaataaagt gaaggccgcg gtggatttgt ttgacgtttt
      421 cgaatcactg gataatttga cccttgctcg tgaactggac aaagagggac agaaacgaaa
      481 gaagatgatt cgtgcgttta tagagatcaa tttgggcggc gaagaaggca aaactggagt
      541 tgtcgaaagc gaggcggcgt cattggtgga acaaatcgcc actctgtccc acttacgcat
      601 cgaagggctc atgagcgtgc cgccgtatcg ggaagatccg gaagaagtgc ggccctattt
      661 tcgaaaactg cgcctgattc aagaaaggct cggcgaaatt tgctctccgc ggctggaact
      721 gcgcgaactc tcgatgggaa tgagccacga ttacaaggta gcaatcgaag aaggtgcgac
      781 tatcgtacgc atcggtaccg cgctcttcgg cccccgacgt gattgattca tgttcgtttt
      841 ggccaacttc ttgttcgcga cggcgcaggt gcttgattat gtgttgtggg catatctctg
      901 gattttagtg gcgcgattcg tcatttctct cgtgaatgcc gattatgaca acccaattgt
      961 gaggttcgtt tacgcagcca cagagccggt gctcgagcga gtgcgcgaga gaatgcctgt
     1021 ctttgccgcc gggtgggatt tatcgccatt ggtcgtatgg attgccatta tcttcctgca
     1081 gcggtttttg gtccggtcgc tctatgactt ggcttattcc atgagataga acggttatga
     1141 tttggctgcg atcgttggtg ccgttgccgc tgagctgcgg agtcgttatt ctcggttttg
     1201 agaggtggcg gcttgtcacg tgggactgaa ttagaccatt ttgccgcagg aataatagct
     1261 taccgtgaag gctgcaaagt attgcaagta aggatcggag gaagggtatg aagatatttc
     1321 ttgctggtaa ctgggtagat aaacccaaaa aaatcgaagt cagaaatccg ttcgataact
     1381 cggtatttga aacagtaccg cacgccgatg ctgaagatct agaaaaggcg cttgcgtacg
     1441 ctgagcgggg cgccaaagtg atggcgaaac tgtcgagcta cgagcgctgg aaaattttac
     1501 ggaaagcggc ggatctgata gcggcacgca acgaggagct tggacaaatt atttccaaag
     1561 aagaaggaaa gattattgct gagggacgag gagaagccaa tcgtgcggtc gaaacaatga
     1621 tgggatcggc ggaagaggct aagcgattgc acggcgagac cgtccccttg gacgctgatc
     1681 ccactggcgg aaagaaattc ggctttactc tgcgtgttcc ttgcggagtc gtcgctgcga
     1741 tcgcgccgtt caattttccg ttgaacttag tcgcgcacaa agttgggccg gcgttggcag
     1801 caggtaactc tgtgatcgtg aagcctgcct ccgatacgcc gctttcggcc ctgaagctta
     1861 cggaaattct tctcgaggct ggacttccgg ccgaaggcat ccaatgcatc accggatcgg
     1921 gaggcgaaat cggcgatgcc ttggtcgccg accgtcgcgt gcgcaaagtg acgtttaccg
     1981 ggagcagaga gataggcgag cgaatctgcc agagggctgg aatcaagaaa gtgacgatgg
     2041 agcttggttc caatagcccc gtcatcatta tgccggatgc cgacctggaa aaggtcgcga
     2101 cagttttggc gatgaccggc tacggcaatg caggtcaaac ctgtatttcg actcagcggg
     2161 tgctcaccgc gaaaagcatt tatggcgatt tattaagtgt catgaagccg aaagtcgaag
     2221 cgctgacgac cggcaaccaa ttggatgaga aatctaaggt aggcccaatg gtgaaagaga
     2281 gcgaagcggt tcgggttgac aagtggatta atgaagctgt ggcgagtggg gcgcgcttag
     2341 tcgctggcgg cggacgccgg ggcgcaatct atacgcccgc ggttgtcgcg gacgtaaagc
     2401 ccaacatgcg gatctcgcgt gatgaactat tcgggcccgc ggtggcagtg acaccgttta
     2461 acgacatcga tgaggccatt agtctcgcaa atgatagcgt atacgggctt gcggctggga
     2521 ttttcaccga gaatgtggaa tgggcgatga agtttgcgcg cgaagc
//
LOCUS       DASULZ010000679         3441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1398175, whole genome shotgun sequence.
ACCESSION   DASULZ010000679 DASULZ010000000
VERSION     DASULZ010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398175"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..1031)
                     /locus_tag="VFY96_14075"
     CDS             complement(246..1031)
                     /locus_tag="VFY96_14075"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5607647.1"
                     /translation="MSEKQMDPIIDLANVTKTYRSKGKSVLALKDVTLPVEKGSFTTI
                     IGPSGCGKSTLLKIIAGISLPYQGRLNYKGREITSINSDIGFVTQDSNLFPWLTLIEN
                     VELALKIRAVSKEQRAQAAQKLIDTMQLTGFETHYPFELSGGMQKRGSIIRTLIYDPD
                     IILMDEPFGPLDAQTRLVLQDELLRIWDTNKKTILFVTHDLTEAIALSDKVVLFTGRP
                     GTVKRIFDVPLARPRNVFEIHNETGFTDLYHEIWSHFKTEIKV"
     gene            complement(1028..1753)
                     /locus_tag="VFY96_14080"
     CDS             complement(1028..1753)
                     /locus_tag="VFY96_14080"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5607648.1"
                     /translation="MFWEIAAWHWPGIARLVSQPIEIARGIVDVVTTGVIWQHFDATL
                     KEMAAGYVIGAITGIALGFLCGRIKLLGDILNPYITLFNGIPKVALAPVFVIWFGIGL
                     MSKIAIILTMVFFVVFINTFAGLRSVNEDYVAIVRIMGASGWQVAREVFLPATLPFIM
                     VGLRAGIPFSVIGAVVGEFIASTKGLGFFINYNQGTYDTNGIFVGVTILALLVVVLDG
                     LLSLLEKRLLKWRPEVEAKNVGA"
     gene            complement(1996..3036)
                     /locus_tag="VFY96_14085"
     CDS             complement(1996..3036)
                     /locus_tag="VFY96_14085"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607649.1"
                     /translation="MKRNRKHLGLLLHVFLFLLCSAPTPLHAQAKAPVKIRLATSTAG
                     LDFAPIWIAQRKGYFKEEGIDFEHILTTGGAVTMAAIMNGDVQFVSTAASDVLVARAR
                     GDRMFSVGIFPASLEWNIATSNKWLESRGLTKSQIAKMPIAQKIQALKGASIGAATVG
                     GAPAQVVRYLLRQNNMKPDSDVRFAAVGAGQARVNALKNGQVDMIVGGIPDTEQPELE
                     GWGYTLIRLGGEIEIFKDYPHESVHALDTYIKAHPDSTRSLLRAIARGNNLIIDNPAE
                     SDDLLVKQFPKIDPSVLKTVMSHSRSTFRRNLRMTKSGWDNMNKVFVAVGLLKSELNT
                     AEGEFWTNQFLP"
     gene            complement(3066..>3441)
                     /locus_tag="VFY96_14090"
     CDS             complement(3066..>3441)
                     /locus_tag="VFY96_14090"
                     /inference="COORDINATES: protein motif:HMM:NF024430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3891 family protein"
                     /protein_id="HEX5607650.1"
                     /translation="AYSDFLSEEHLWTNFKLMEVYDQMGQFVCNRYPFNSTQRKNGPS
                     NTMSNVPVPTRPGKDDTILTFTIKDESHAAVAPYPFDIDPLTVSFQGRLIPKRRYLSQ
                     NEFLQEYYGAEKLAINYLLSSN"
BASE COUNT          749 a          892 c          993 g          807 t
ORIGIN      
        1 tatagatccc ataggcgcca tcggtggttg gtccggagaa caagaggcgt ccggccttgt
       61 gctgttgctc gagccaaagg cgatgttcgt cgtaatgggc ctgtttgtct tgttccgttt
      121 ccggcttcga tcgcgaaaga acgatgtacc acatgatcgt ttgtcctttc gtatgagaaa
      181 taattacatt tgatttttca acgctgacgc tagctcactt ggttttgaac gggtttatcg
      241 ccagattaaa ctttgatctc ggttttaaag tgactccaga tttcgtgata gagatcggtg
      301 aaacccgttt cgttgtggat ctcaaaaacg ttgcgcggtc gcgcaagcgg cacgtcaaag
      361 atccgtttaa ccgtgccggg ccggccggtg aacaaaacga ccttgtccga aagcgcgatc
      421 gcttcggtaa gatcgtgggt gacgaacaga atcgtttttt tgttcgtatc ccaaatgcgc
      481 aggagctcgt cttgcagaac caaacgggtt tgcgcgtcaa ggggaccgaa tggctcgtcc
      541 atcaaaataa tgtcgggatc atagattaac gtgcggatga tcgagccgcg cttctgcatg
      601 ccgccggaga gctcgaaagg gtagtgagtc tcgaagccgg tcaactgcat ggtgtcgatg
      661 agcttttgcg cggcctgagc gcgttgctcc ttggatactg cgcggatctt cagcgcgagc
      721 tcgacgtttt cgattaatgt cagccaaggg aacagattgc tgtcctgagt gacgaaaccg
      781 atatcggaat tgatcgacgt aatctcccgt cctttatagt tgagccgccc ttggtagggc
      841 aacgaaatcc ccgcgataat cttgagcaag gtgctcttac cgcagccgct cggtccgatg
      901 atggtggtga acgagccttt ttccacaggc agcgtgacgt cttttaaagc gagaacgctt
      961 ttgcctttag accggtaggt cttggtgacg ttggcgaggt cgatgatcgg atccatctgc
     1021 ttttcgctca tgcgccgacg ttcttggctt cgacttccgg gcgccatttc aacaggcgtt
     1081 tttccaaaag cgacagcaag ccgtcgagta cgaccacgag cagtgccaga atcgtcacgc
     1141 cgacgaaaat cccgttggtg tcgtaggtgc cctggttata gttgataaaa aatcccagcc
     1201 ctttggtgga agcgatgaat tctcctacca cggcgccgat gacggagaac ggaatgcccg
     1261 cgcgcaagcc gaccatgata aacggcagtg tggccggcaa aaagacttcg cgcgcgactt
     1321 gccaaccaga ggcacccatg atgcggacga tcgcgacata gtcttcgttc accgagcgca
     1381 ggccggcgaa ggtgttgatg aacacgacga agaaaaccat ggtcaagatg atggcgatct
     1441 tcgacatcaa gccgatgccg aaccagatca caaagaccgg cgccaaggcg acttttggaa
     1501 tgccgttaaa aagcgtgatg tagggattga gaatgtcgcc gagcaatttg attcgcccgc
     1561 aaagaaaacc gagtgcgatt ccggtaatcg cgccgatgac atagccggcc gccatttctt
     1621 tgagcgttgc atcgaagtgc tgccagatca cgccggtcgt tacgacgtcg acgatgccgc
     1681 gggcaatctc gatcggctgg ctcaccagcc gcgcgatacc cggccagtgc caggcggcaa
     1741 tttcccaaaa cacgagtgtg cagatgccag ggagaaggac gagaaggcta cgaaagttcg
     1801 agagcgtgaa gtttttttgc gctagtccgg catggttctt atctgctaat tcggtggatg
     1861 cagaagttgg agcagtacga tcttccataa agttattttt cgagcgcgat ccttgaatac
     1921 tcctgattcc cggtcgggca gccccggact agttcccggg ttgcccgcaa tgaggatgca
     1981 cctctgttgc ggacgctagg gcaaaaactg gttggtccag aactcaccct cagcggtatt
     2041 cagctcggat ttgagtagtc cgaccgcgac aaaaaccttg ttcatattgt cccaacccga
     2101 tttggtcatc cgcaggttgc gccggaaggt cgagcggctg tggctcatga cggtcttcag
     2161 aaccgatggg tcgatcttag gaaattgctt gaccagtaga tcgtcggact ctgcgggatt
     2221 gtcgatgatc aagttgttgc cgcgcgcaat cgcccgcagc aaagaccgcg tggagtcggg
     2281 gtgcgccttg atgtaggtat cgagcgcgtg cacggattcg tgcgggtaat ctttgaaaat
     2341 ttcgatttcg ccgcccagac ggatcagcgt gtagccccat ccctcgagtt cgggctgttc
     2401 ggtgtccggg atgccgccga cgatcatatc gacctggccg ttcttgagtg cgttcactcg
     2461 ggcttgacca gcgccgacgg cggcgaaacg cacgtccgag tcgggcttca tgttgttttg
     2521 ccgcagcagg tagcgcacca cttgcgcggg cgcgccgccg accgtggcgg cgcctatgct
     2581 cgcgcccttg agcgcctgga ttttttgcgc gatcggcatc ttcgcgatct ggcttttcgt
     2641 caggccgcgg ctttccagcc atttattact ggttgcgatg ttccactcga gtgaggcggg
     2701 aaaaatgccc acggaaaaca tgcgatcgcc acgcgcgcgg gcgacgagca catcggaagc
     2761 cgccgtcgaa acgaattgaa cgtcgccgtt catgatcgcg gccatggtga cggcgccgcc
     2821 agtggtcaag atatgttcaa agtcgatgcc ttcttctttg aagtagcctt tgcgctgggc
     2881 aatccagatc ggcgcaaaat cgagcccggc ggtggatgtc gccaacctga ttttaaccgg
     2941 cgccttggct tgtgcgtgga gcggcgtcgg cgcgctgcat agcaaaaata aaaacacgtg
     3001 caaaagtaaa cccagatgtt tacgatttcg tttcactagg agttcctccc ggaacaatcg
     3061 ctcagctaat tcgacgaaag caaataattg atcgccaatt tttcggctcc atagtattcc
     3121 tgcaaaaatt cattctgact cagatagcgg cgcttgggta tcagccgtcc ctggaacgag
     3181 acggtcaatg gatcgatgtc gaacggatag ggcgcgaccg cggcgtggct ttcgtctttg
     3241 atcgtaaacg tcaggatcgt atcgtctttg ccgggccggg taggcacggg aacgttgctc
     3301 atagtattgc tgggtccatt tttccgctga gtgctgttga acggatagcg attgcagacg
     3361 aactggccca tctgatcgta gacttccatg agcttgaagt tcgtccatag gtgttcttcg
     3421 gaaaggaagt cgctataggc c
//
LOCUS       DASULZ010000680         3010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1295847, whole genome shotgun sequence.
ACCESSION   DASULZ010000680 DASULZ010000000
VERSION     DASULZ010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295847"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /gene="fusA"
                     /locus_tag="VFY96_14095"
     CDS             complement(<1..1259)
                     /gene="fusA"
                     /locus_tag="VFY96_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX5607651.1"
                     /translation="MARTVPLEKTRNIGIMAHIDAGKTTTTERILYYTGINYKIGEVH
                     EGTATMDWMVQEQERGITITSAATTCHWRDHRINIIDTPGHVDFTIEVERSLRVLDGA
                     VAVFCSVGAVEPQTETVWRQADKYRVPRLAFVNKMDRLGADFFRVVKMIEDRLGARTV
                     VMQLPIGAEDKFSGIVDLVTMKAVVWEDESLGAKFHEEPIPSELKAEAEEYREKLVEA
                     AADCDENIMMKYLDGKEVTETEIRSAIRSGTLSLKVVPILCGSAFRNKGVQPMLDAVV
                     DYLPSPLDIPPVTGVEPGTTKPAERPAKDDAPFSALAFKIMTDPFVGTLTFFRVYSGT
                     LNSGASIYNSTKSKRERIGRLLKMHANKREEIKEVYAGDIAAAVGLKTATTGDTLCDE
                     NKPIVLESIDFPDPVISIAIEPKSKAD"
     gene            complement(1329..1799)
                     /gene="rpsG"
                     /locus_tag="VFY96_14100"
     CDS             complement(1329..1799)
                     /gene="rpsG"
                     /locus_tag="VFY96_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX5607652.1"
                     /translation="MPRKGEVRKREILPDPKYGDKVVAKFVNTIMSRGKKSVAESIFY
                     RSLDIVTAKAKEDALGVFKRAIENTRPSVEVRSRRVGGATYQVPVEVRPQRRLSLSMR
                     WLIDSARERAEKSMEEKLAAELMDAANNRGTAIKKKEDTHRMAEANRAFAHYRW"
     gene            complement(1807..2178)
                     /gene="rpsL"
                     /locus_tag="VFY96_14105"
     CDS             complement(1807..2178)
                     /gene="rpsL"
                     /locus_tag="VFY96_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX5607653.1"
                     /translation="MPTISQLVRESREKQRPKNTAPALQGCPQKRGVCTRVYTSTPKK
                     PNSALRKVARVRLTNGIEVTSYIPGVGHNLQEHSVVLIRGGRVKDLPGVRYHIVRGTL
                     DSIGVQERRQGRSKYGAKRPK"
     gene            complement(2285..>3010)
                     /locus_tag="VFY96_14110"
     CDS             complement(2285..>3010)
                     /locus_tag="VFY96_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX5607654.1"
                     /translation="RKPKEFAVITEIDGVVSFGKDTKGKRKVIVTPEVGEPREYLIPK
                     GKHISVHDGDKIRAGEALMDGSSNPHDILNILGEKALAKYLVDEVQEIYRLQGVRIND
                     KHIEVIVRQMLRRVRIKEVGDTEFLIGDQVEKWRFDEENQKAITERGKPAVAEPLLLG
                     ITKASLSTESFISAASFQETTKVLTEAAIHGKVDLLCGLKENVIMGRLIPAGTGLTKY
                     AKAEIEIDEPESPGGVTPEAAEA"
BASE COUNT          588 a          891 c          775 g          756 t
ORIGIN      
        1 tgatccgctt tgcttttggg ttcgatcgca atcgagatga ccgggtccgg gaaatcgatg
       61 gattcaagga caatcggctt gttttcatcg cacaacgtgt cgccggtggt cgcggtcttt
      121 aaacccacag ccgcggcaat atcacccgca tagacttctt tgatctcctc gcgtttgttc
      181 gcatgcattt tcaacaatcg gccgatgcgc tctcgcttgc tcttggtcga attgtagatg
      241 ctggcgcccg aattcagcgt gccggagtag acgcgaaaaa aggtcagtgt tccgacaaaa
      301 ggatcagtca tgatcttgaa agcaagagca gaaaaaggcg catcatcctt ggccgggcgc
      361 tccgccggct tcgtggttcc gggctccacg cccgtgaccg gaggaatatc aagcggcgac
      421 ggcaggtagt cgaccacagc gtccaacatc ggctgaacgc ctttgttacg aaacgcggag
      481 ccgcagagaa tcggcacgac tttaagcgac aacgtgccgc tacgaatcgc cgatcggatc
      541 tctgtctcgg tgacctcttt gccgtcgaga tatttcatca taatgttctc gtcgcaatcg
      601 gcggccgctt cgacgagttt ttcccgatat tcttccgcct cagccttgag ctccgacggg
      661 atcggctctt cgtgaaattt agccccgaga ctctcatcct cccataccac cgccttcatc
      721 gtcactaaat cgacaatgcc ggaaaacttg tcttcagcgc caatgggcaa ctgcatgacc
      781 actgtgcggg cgcctaaccg atcttcgatc atcttcacaa cgcggaaaaa atcggcgccg
      841 agccgatcca tcttgttgac gaacgcgagc cgcggcacac ggtacttatc ggcctgccgc
      901 cacacggttt cggtctgcgg ctcgactgcg ccgaccgagc agaaaaccgc aaccgctccg
      961 tccaagactc gcaacgagcg ttcgacttcg atggtaaagt ccacgtggcc gggtgtgtcg
     1021 ataatattga tccgatgatc gcgccaatga catgtggttg cggcagaagt aatcgtgatg
     1081 ccacgctcct gctcttgcac catccagtcc atggtcgcgg taccctcgtg cacttcgccg
     1141 atcttgtagt tgatcccggt gtaatagaga atccgctcgg tggtcgtcgt tttacccgca
     1201 tcgatgtgcg ccataatccc gatgttgcgg gttttctcta ggggaacagt tcttgccatt
     1261 tttcttctac gcggaaaaaa aggaggcggt caacccgcct ccttttcctg tctcgctcaa
     1321 tgagtggact accaacggta atgggcaaac gcccgattgg cctcggccat acgatgggta
     1381 tcttccttct tcttaatcgc cgtgccgcgg ttattagcag cgtccatcaa ctcggccgct
     1441 aatttttctt ccatcgactt ttccgcccgc tcgcgcgctg aatcgatcaa ccatcgcata
     1501 ctgagcgaca gtcgccgctg tgggcgtacc tctacgggga cttgatatgt tgcgccgccc
     1561 acacgtcggg aacggacttc aacgctcggc cgggtatttt cgatcgcccg cttgaatact
     1621 cccagcgcgt cttctttggc tttggcggtc acgatatcga gcgatcggta aaagatcgac
     1681 tcagccacgc tctttttgcc gcggctcata attgtgttca cgaacttggc tactactttg
     1741 tcgccgtact tgggatcggg caaaatctct cgttttcgaa cttcgccttt gcgtggcata
     1801 cgtttcctac ttcggtcgct tcgcgccgta cttcgaacgg ccctggcggc gctcctgaac
     1861 accgatcgag tccaaggtcc cgcgcacgat gtgatagcgc acgcccggca ggtccttgac
     1921 gcggccacca cgaatcaaaa ccaccgagtg ctcctgcagg ttgtgcccca cgccaggaat
     1981 atacgaggtc acctcgattc cattggtgag tcgcacgcgc gccactttgc gcaacgccga
     2041 gttcggcttt tttggggttg aggtgtatac ccgagtgcag acgccgcgtt tttgcgggca
     2101 gccttgcaga gccggcgcgg tgtttttcgg ccgctgtttt tcccggcttt cgcgcactaa
     2161 ttgactaatc gtcggcatat tttctcttca aaatgaatgg atttctgagg agctcagggt
     2221 actctgaact cctctaggaa aatcctgtta ttaacaaatt agggcagtgt tgtcaaaact
     2281 cgcttcaggc ctctgccgct tcgggtgtca ctccacccgg actttccggc tcatcgattt
     2341 caatttcggc tttcgcgtac ttcgttaaac cggtgccggc tggaattaaa cgacccataa
     2401 tgacgttttc tttaaggccg caaagcaaat ccactttgcc gtgaatggcc gcttccgtaa
     2461 gcaccttcgt cgtctcctgg aaggaggccg cagagatgaa gctctcggtc gacagcgacg
     2521 ccttggtgat tccgagcaac agcggctcgg cgacggccgg cttaccgcgt tcggtgatcg
     2581 ccttttggtt ctcttcgtcg aagcgccact tttcgacctg atcgccgatc aaaaactcgg
     2641 tatcgcccac ttctttgatg cgcacgcggc gcagcatctg tcggacaatc acctcgatgt
     2701 gcttgtcgtt gatgcgcacg ccctgtaacc gataaatttc ctgcacttcg tcgaccagat
     2761 atttggccag cgccttttca cccaggatgt ttaagatatc gtgaggattc gacgagccgt
     2821 ccatcagcgc ttcaccggct ctgattttgt cgccatcgtg cacgctgata tgtttgccct
     2881 tgggaatgag atattcgcgc ggctcaccca cttcgggagt tacgattact ttgcgtttgc
     2941 ccttggtgtc tttaccgaac gagacaacgc cgtcgatctc ggtgatcacg gcgaactctt
     3001 tcggcttccg
//
LOCUS       DASULZ010000681         2401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_586121, whole genome shotgun sequence.
ACCESSION   DASULZ010000681 DASULZ010000000
VERSION     DASULZ010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586121"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VFY96_14115"
     CDS             complement(<1..510)
                     /locus_tag="VFY96_14115"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF045191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX5607655.1"
                     /translation="MSTISTLAYKDLRGYIDAVEQLGELRVIHGADWDLELGAITEVA
                     ARATSPKVLLFDDIKGYPSGFRVLTNPVCSKTTTALAFGLEPKLTGLEIIRAWKEKLG
                     GQKPLKPVEVSNGAITENVEKDVDLLKFPTPRWHEDDGGRYIGTGCMVIMRDPQENWI
                     NVGTYRVCVH"
     gene            complement(640..1014)
                     /locus_tag="VFY96_14120"
     CDS             complement(640..1014)
                     /locus_tag="VFY96_14120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607656.1"
                     /translation="MIEHTVGWGQTDAQANWRKAPKREGKLIWYTSTNVTESKPFLYD
                     FEKQYPFIKGEICRASGEKTLNRIITEARAGRSEFDIVPISEVDALIEAKILAPYRSQ
                     VKPKNLDFAVGKKTDMLRYGSA"
     gene            complement(1379..2362)
                     /locus_tag="VFY96_14125"
     CDS             complement(1379..2362)
                     /locus_tag="VFY96_14125"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX5607657.1"
                     /translation="MSDDEIRLKPTRLRTHPHTTRSRLMLEVASQLVSRKDFPYLQAR
                     IQLREQGSAEWPLTMFASDSPATIHEVAVGNVQFAIINPSMILKIAALGSAPFKEPMP
                     LRVIAVLPSLDQMVFAVKRETGLTSFSDIGKRRFPLRVSLRAQPDHSLHLIIDHVMRA
                     AGFTLADIISWGGKVRYDAGMAYGPNRIGAIMRGEIDAIFDEGASAWGNMALELGMNF
                     LSLDEDLLQEVEASGLRRGIIPRASFPKLDGDVPTLDFSGWPIYTNANTPDGMVSDFC
                     RALDESKERIPWAQDQPLPLATMVRDTPDGRLEVPLHPAAEGFWRQCGYLP"
BASE COUNT          538 a          678 c          635 g          550 t
ORIGIN      
        1 gtgaacacaa acgcgatacg tgccgacatt gatccagttc tcttgcggat ctcgcataat
       61 gaccatgcag cccgtgccga tatagcgccc gccgtcgtct tcatgccaac ggggtgtggg
      121 aaatttgagc aaatcgacat ctttttcgac gttctccgtg attgcgccat tggaaacctc
      181 caccggcttc aatggttttt ggccgccgag cttttctttc catgcacgaa taatctccag
      241 accagtgagt ttcggttcta aaccgaatgc cagcgccgta gtggtctttg aacaaacggg
      301 atttgtaagc accctgaagc cgcttggata ccctttgatg tcatcgaaca acaatacctt
      361 ggggctggtc gcacgcgcgg ccacttcggt gatcgcgccg agctccaaat cccaatccgc
      421 gccgtgaatg acgcgcaact cgccgagctg ctcgaccgca tcgatatagc cgcggagatc
      481 tttataggcg agtgttgaaa tcgtagacat ctggcatcct ccttcggtta ttcccctcgc
      541 cgacgattgc gagagggcga gagtgaggaa ttcgtgcaaa cgccctcgtc ctaaactgtt
      601 tccagaggga ctttgttaaa tcgacaaagt acggatgtgt catgctgagc cgtagcgaag
      661 catgtcagtt tttttgccaa ccgcaaaatc cagattcttc ggtttcacct gcgagcgata
      721 cggagcaagt attttggcct caatcagtgc atcgacctcg ctgatcggta cgatatcgaa
      781 ctccgaccga cccgcccgcg cttcagtgat gattcgattg agcgtttttt cgccgctggc
      841 gcggcagatc tcgcctttga tgaacgggta ctgcttttca aaatcataga gaaacggctt
      901 cgattccgta acattggtcg aagtgtacca aatgagtttg ccctcccttt ttggcgcctt
      961 ccgccagttg gcttgagcat ccgtttggcc ccaaccgaca gtgtgctcaa tcaaaagaaa
     1021 aaagccacga attcgacaat gccgcaattc ttgaaactga actcatcgcg cgatgccttc
     1081 gtgtcacaaa ttcgttcagg tacaaagctg cgtgagtata agaaacaata ggtttaagtc
     1141 aattagcttg ggccgaacag gtgaaatcac cttccgcctc cgcaaagttc attaattctc
     1201 attccgagca gaggaccgga gggcgaatgc cattcgcccc tccagcggat gttccttggc
     1261 ttctctgcgc cgatgcactc tcagcctcgg aggattcgag ataacaggca ctgtcatttc
     1321 gagcagagcg aggaatcttc cttccctatt cggggatcag ggcagccgcc ggtcgcccct
     1381 agggcaaata gccgcactgg cgccagaagc cctcggcggc gggatggagg ggcacttcga
     1441 ggcgcccgtc cggagtgtcg cgtaccatgg ttgctagcgg cagcggctgg tcctgagccc
     1501 agggaatgcg ctctttgctt tcgtccagcg ctcggcaaaa atcgctgacc atcccgtcgg
     1561 gtgtatttgc attcgtgtaa atcggccagc cgctaaaatc gagcgtcggc acgtcaccat
     1621 cgagtttggg gaaactcgct cttgggatta tcccgcggcg cagaccggac gcttctacct
     1681 cttgcagaag atcttcatcg agcgacagaa aattcatccc aagctcgagc gccatattac
     1741 cccacgcgct cgccccttca tcgaaaatcg catcgatctc gccgcgcatg attgcgccga
     1801 tgcggttcgg gccgtaggcc atgccggcgt cgtagcgcac cttgcccccc cacgaaataa
     1861 tatccgcgag agtgaatccg gcagccctca tcacatgatc gatgatcaga tggagcgaat
     1921 gatcgggttg cgcgcgcagc gatacgcgta aaggaaatcg ccgcttgcca atgtcactga
     1981 atgaagtcaa gccggtttcg cgcttcacgg caaaaaccat ttgatccaat gacggcagca
     2041 ccgcaatgac gcgaagcggc atcggctctt tgaacggcgc gcttccgagc gcggcgattt
     2101 tgagaatcat ggatggatta atgatggcaa actgcacgtt gccgacggct acttcgtgaa
     2161 tggtcgcggg gctgtcgctc gcgaacatag tcagcggcca ttccgccgaa ccctgttcgc
     2221 gcaactgaat gcgcgcctga agataaggaa agtcttttcg actgaccagc tgcgaagcga
     2281 cttcgagcat cagacgggat cgcgtcgtgt gtggatgggt gcgcaaccgg gtcggtttca
     2341 gacgaatttc gtcgtcgctc atatcgggca attacttacc accaactgat tcataaagtt
     2401 a
//
LOCUS       DASULZ010000682         3044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_79345, whole genome shotgun sequence.
ACCESSION   DASULZ010000682 DASULZ010000000
VERSION     DASULZ010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79345"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1284)
                     /locus_tag="VFY96_14130"
     CDS             complement(61..1284)
                     /locus_tag="VFY96_14130"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX5607658.1"
                     /translation="MTAGSPLRIGKKRANELARADKTCAKNGVNGRPENFHPVPIPLL
                     DLTRKYRSIDAELHRQWNLTFESMRLLNGRNLAAFEEEFAAYCNVRHAIGVGSGTDAI
                     FLSLRALGIGAGDEVILPAHAPAPVIEPILYVGATPVLIDKASGDYGPDLAGLRDAIT
                     AHTKAVLAVHLLGLPCDMDAIGHIVSETGVPVVEDSSQAQGAMYRGKRAAGLGAITPM
                     SLGPVKNLACYGDGGVVLTNDGELARTVRLLRVHGQSEKYNHKIYGWNSRLDELQAAV
                     LRVKLPFLDRDNARRRDIADAYTRELSRFRLKTPPVFDDRQSVYHQYVIETPYRDELK
                     SFLAARQIGTGIYYPVPLHRHEAWVSRGFRQLSLPEAERYSSQNIALPMFAELTDDEI
                     DWVIGAVKEFFSAYD"
     gene            complement(1405..1701)
                     /locus_tag="VFY96_14135"
     CDS             complement(1405..1701)
                     /locus_tag="VFY96_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607659.1"
                     /translation="MFAKLVALSVLTVWFALIGLGYAASDNVDTYIVPSIVALDNQDY
                     GDDVDNYRPIDLVSLPALFFLNLPQLSLERSFPRIAKISSDPIKCFELNCSLLL"
     gene            1855..2901
                     /locus_tag="VFY96_14140"
     CDS             1855..2901
                     /locus_tag="VFY96_14140"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5607660.1"
                     /translation="MNISIKLFLFSASFFLLAHDRLLFAQSDFYKGKTITVYIGTTAG
                     ALYDQWGRILAQHMGKHIPGKPDMIVQNMPGAGHMIAANYVYNKTKPDGLSLIGSIVP
                     TLYFDQLVGRKEAQYDWAKFVWIGSPVQGESQMYMRADTPYKTIEDVRNAKEPPRCGA
                     QGTSDSAYYLPKLFEETIGTKFNLVQGYPGGPEIDLAVERGEIHCRAFTIEAFMSREP
                     YHTWRKKGFVRNIIQTGKKRDAKLPETPTLWELMDRYKTPDPSRRLATLMLASGQLGR
                     PMMATPGVPADRVKILRDAFNATMKDSEFLADIDKRQFDLDPVPGEQLEKIVKDVMTQ
                     PPDIVERMKKLLGN"
BASE COUNT          736 a          812 c          821 g          675 t
ORIGIN      
        1 ttcccttctt cgttatgcat cggggcaatg acggagatgt cgatgcgcgc tccatctcga
       61 tcagtcgtaa gcagaaaaga actccttgac ggcgccgatc acccaatcga tttcgtcatc
      121 cgtcaattcg gcgaacatcg gcagggcgat attttgactg gagtagcgtt ccgcctcggg
      181 caacgaaagc tggcgaaaac cgcgactcac ccaggcctca tgacgatgca gcggtactgg
      241 atagtaaatc ccggtcccaa tctgtcgcgc ggcaagaaaa cttttcagct catctcgata
      301 cggcgtttca atgacatatt gatggtacac actctgccga tcatcgaaga ccggcggcgt
      361 tttaagccgg aatcgggaaa gttcccgagt atatgcgtcc gcgatatctc gccgtcgcgc
      421 attgtcgcgg tcgaggaacg gcagttttac tcgcagcacc gcggcttgca attcgtccaa
      481 gcggctgttc cagccgtaaa ttttatgatt gtatttttcc gactggccat gcacgcgcaa
      541 caatcgaacc gttcgcgcca gctcgccgtc attggttagc acgaccccgc catcgccata
      601 gcaggccaga tttttgaccg ggcccaaact catgggggtt atcgctccga gtcccgccgc
      661 gcgtttgcca cgatacatgg cgccctgcgc ttgcgacgaa tcttcaacga ctggaactcc
      721 agtctcgctg acgatatggc cgattgcatc catgtcgcag ggcaacccaa gaagatggac
      781 ggccaggacg gccttggtat gggccgttat ggcgtcccgt aaaccggcca ggtcgggtcc
      841 gtaatcgccg ctcgctttgt cgatcaaaac gggtgtggcg cctacataaa gaattggctc
      901 gatcacgggc gccggcgcat gagccggcaa aatcacttca tcgccagcac caatacccag
      961 cgcgcgcagg gataaaaata tcgcgtcggt gccggagccc acgccgattg catggcgaac
     1021 gttgcagtat gcagcaaatt cctcctcgaa tgcggcgaga ttgcggccat tcaacaaacg
     1081 catggactcg aaggtcagat tccattgccg gtgcaattcg gcgtcaatgg aacggtactt
     1141 tcttgtgagg tctaacagcg gaatgggtac gggatgaaag ttttcggggc ggccattgac
     1201 accatttttt gcgcaagttt tatcggcgcg agctagctcg tttgcccgct tctttccgat
     1261 ccgtagtggt gagcctgctg tcatccaaat tctccttcag acggtctgcc acgcctagaa
     1321 ctagtggcgg ccgagtttcg cacgactgcc aaaatacagt cggcgttaac tcccggtgtc
     1381 gcgagtgcga gtatagagcg ccggtcagag aagcagggag cagttcagtt cgaaacactt
     1441 aatgggatct gacgaaatct tcgcaatacg gggaaaggat cgctccaatg aaagttgcgg
     1501 taggtttaaa aagaagagag cgggtaaact tacgaggtcg atgggccggt agttatcgac
     1561 atcgtcgcca tagtcctggt tgtctagagc aacaatggat ggcacgatgt aggtgtccac
     1621 gttatcgctg gccgcatatc caaggccgat cagtgcaaac caaacggtaa gcaccgacaa
     1681 cgcgacaagc ttggcaaaca tgaaaggtat atataccctc gtttgccaag tcacaagaga
     1741 acgtcgggtt gtttcggtca aacgagcagt ggtgagacca gagcgttgat cgctgcttga
     1801 cagcgacata gcgcgtgttt aacttacgac atcaactttc cagaggagtt ctgtgtgaac
     1861 atcagtatca aactattttt attctcggcc agtttctttc tgctcgctca cgaccgtttg
     1921 cttttcgctc agagtgactt ttacaaagga aaaacaataa cggtctatat cggcacgacg
     1981 gccggggcat tgtacgatca gtggggccga attctcgcgc agcatatggg gaaacatatt
     2041 cctggcaaac ctgacatgat cgtgcagaat atgccgggcg cgggacacat gattgcagcc
     2101 aattacgtat acaacaaaac taaaccggac ggcttgagtc tgattggctc gatcgttcct
     2161 acgctttact tcgatcaatt ggtcggtcga aaggaagcac aatatgattg ggcaaaattt
     2221 gtctggatcg gctcgccggt tcaaggtgaa tcgcagatgt atatgcgcgc cgataccccg
     2281 tataagacca tcgaagatgt ccgcaatgcc aaggaaccgc cgcgttgcgg tgcgcaggga
     2341 acttcggact ccgcgtacta tttacccaaa ctgttcgagg aaaccattgg cacaaaattc
     2401 aatctcgtcc agggctaccc gggcggccct gaaatcgatc tcgcggttga gcgcggagaa
     2461 atacactgcc gagccttcac tatcgaagct ttcatgtcgc gcgagcccta tcacacctgg
     2521 cgcaaaaaag ggtttgtgcg caatatcatt caaaccggca aaaaaagaga tgcaaaactg
     2581 ccggagacac cgacgctctg ggagttgatg gatcgctaca aaacacctga cccgagccgc
     2641 cgcctcgcga cgcttatgct tgcttccggc cagctcggca gaccgatgat ggccacgccg
     2701 ggtgtccccg cggaccgggt caaaatttta cgggacgcct tcaacgcaac catgaaagat
     2761 tcggagtttc tcgctgatat cgataagcgg caatttgatc tcgatcccgt gcccggcgaa
     2821 caactggaga agatcgtcaa agacgtgatg acccagccgc cggacatcgt cgagaggatg
     2881 aagaaactgc taggcaactg actagggttg cgaaatcggt gcttcaagca gcgccgtgat
     2941 tcggcgccgc ttttttttaa tccagcgggc ttcgaacgga agttctcgga acgtggcttc
     3001 cttccgcttg aaagtttgtg ctaagagctt ttcatggaaa aatc
//
LOCUS       DASULZ010000683         3984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1499143, whole genome shotgun sequence.
ACCESSION   DASULZ010000683 DASULZ010000000
VERSION     DASULZ010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499143"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VFY96_14145"
     CDS             complement(<1..205)
                     /locus_tag="VFY96_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607661.1"
                     /translation="MAQDAKEFRHQTPDFPGVKKTWHVCNTDLMKVQVQVVKNGGENN
                     LHTHTGEDAFWYVISGAVKFYGEG"
     gene            355..1341
                     /locus_tag="VFY96_14150"
     CDS             355..1341
                     /locus_tag="VFY96_14150"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607662.1"
                     /translation="MKKNWMSLMAAPIFIFSTATTWGQSLPLLNMGFSGAGIGADLLK
                     VIDRSALWRKQGLEVRTVYLSSGTLMAQTLSSGDIGIAGFDTPAMLNLALAGHSLKVI
                     AVPINRLEPFFVVRSNIKTPADLKGKKITISRFGSGSDIMTRVALKYWKIDPDKDVSF
                     FQSGNTPSRTAALVAGHMDGALVSSTGVAKIVATGCCRVLADLSELPLEFANYGVVVS
                     GSLLKNQRDNVKRFLQALIEGIHTFKNSSELARAVLRESNGDAEVGALYERLTKSFRD
                     FPSPENRGIQNVIDSLPTPKARGAKAEDFIDRTLMEEIRKSGFMEKLGGAKG"
     gene            1515..2864
                     /locus_tag="VFY96_14155"
     CDS             1515..2864
                     /locus_tag="VFY96_14155"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX5607663.1"
                     /translation="MSKTLITNALIVPMTERRSFNGYVRIENGAIAAVDCGTAPEVLP
                     EEQVIDAAGAVLMPGLVNAHTHLYQVLLRAVWEDLELLPWLRRIYGCARVLRPEHFYA
                     GSLLGCVEALRSGVTTICEHNFLNPSPDCAFETIRAIEDSGVRGVFARTIMDTGEIVP
                     DCTKEKPEAAFREIENILSQHQNRDRLSFMTGPNTPPINTTPELLRELRQFADDHLLG
                     ISAHVAESKSVVQSVKNQYGKNGVVEFLAQFGIPAANSIFAHSVHVSQREIDVLRETG
                     TSVSHNPVSNMMLGDGIAPVVEMLRQGVNISLGTDGAASNHSQDLFETMKAASLLQKV
                     HHQDAGVIDPFSVLRMATAGGAKALGLDSVCGTVEVDKRADLMLVDIDTVHNQPVNDI
                     FSQLVHCAKASDVHTVIIDGEILMRDQQFHRIDERRVLADAKVAHRDLMERVSQVSF"
     gene            complement(2893..3954)
                     /locus_tag="VFY96_14160"
     CDS             complement(2893..3954)
                     /locus_tag="VFY96_14160"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX5607664.1"
                     /translation="MTLILSNADVQNALDVAECLAVMAESYREQAASRAANRPTCHTY
                     LPHSLPNATYSFKSVDGAIGKYGVLALRVTSDIVQEQVVHGSMRLEKLPLAGHGMFVG
                     LVQLFSAETGELLAIMPDGLIQQTRVGVTSALAMRVMARKNSQVLGVIGSGGQAKAHY
                     RFMTAVMALKKVKVYSPNPGHRTKFVEEMTKETGVAGEAVDTVEQAATDVDVVCTATN
                     ASQPVLNIDWLKPGMHYNAIREFETDLSVLERCEVVAIHTNLGGIQHYQPPGILDDMP
                     GVRREKPRDWSKYPEICDLIAGKAPSRTDDQQITFFLNNVGTGVQFAAMGYCAYKGAR
                     EKGLGREIPSEWFMQDIKP"
BASE COUNT          911 a         1043 c         1102 g          928 t
ORIGIN      
        1 cgccctcacc atagaacttg accgcaccac taatgacata ccaaaaagcg tcttcaccgg
       61 tatgggtatg caagttgttt tcaccgccgt ttttaaccac ttggacctgt actttcatga
      121 gatcagtgtt acagacatgc caggtctttt ttacgccggg aaaatcggga gtctggtgtc
      181 gaaattcttt tgcatcctga gccatgagtc acctcgcgtc aaaatttgcc ttaccttagt
      241 ccggttcagg cagcacggtc aagcgtcggt tcaacgggat tccgtgcatt gacggcgtaa
      301 tgaggactcg ctaacttgag gacgccaaaa ttccgtgcgc caagggagca aaacttgaaa
      361 aaaaattgga tgagcttgat ggcggctccg atttttattt tcagcacagc aacgacgtgg
      421 gggcagagtc tgccgcttct caacatgggc tttagcggcg ccggcatcgg tgccgatctg
      481 ctcaaggtca tcgatcgaag cgcgctctgg cgaaagcagg gtctcgaagt gcggacggtt
      541 tatctttcga gcggtaccct gatggcacag acgctgtcct ccggcgatat tggcatcgcc
      601 gggtttgata ctcccgcaat gctgaacctt gcgttggctg gacatagcct gaaagtgatt
      661 gcggttccga tcaatcggct tgagccgttt tttgtcgtgc gaagcaacat caaaactccc
      721 gctgatttga aaggtaagaa aatcaccatc agccgctttg gctcgggctc ggatatcatg
      781 actcgagtgg cgctcaaata ctggaaaatc gatccggata aagacgtaag ctttttccaa
      841 tcggggaaca cgccaagccg tacggccgcg ctcgtcgccg gccacatgga cggggcgttg
      901 gtgagctcga ccggcgtggc gaagattgtc gccactggtt gttgccgcgt gctcgcggat
      961 ctgtccgagc tacccttgga gtttgccaat tacggagtgg tggtctccgg gtcgttgctc
     1021 aaaaatcagc gcgataacgt gaagcgattt ttacaggcgc tcattgaggg aatccacacg
     1081 ttcaagaaca gttcggagct tgcgcgggcc gttttgcggg aaagtaatgg tgacgctgaa
     1141 gtcggtgcat tatacgagcg gctgaccaaa tcttttcgag attttccatc gcctgaaaat
     1201 cgcggcattc aaaatgtcat tgattcgctg ccgacgccga aggcgcgcgg cgccaaggcc
     1261 gaggacttca tcgatcggac cctgatggaa gagatccgca agagcgggtt catggagaag
     1321 cttggcgggg cgaagggcta gtcggcggtt ggaagtatga gccgccagtt tgtcatctcg
     1381 agcgccagcg agagagctta tattcagagt tagatgcgaa gaaaatattt cgcctctggc
     1441 tcgaaataac cgtcacgaaa cggagaacac tgaaacaagg cagtgaaact taatccgctt
     1501 agttgagaat ttttatgagc aagacactga ttactaacgc gcttattgtc ccaatgacgg
     1561 aacgccgctc tttcaatggt tacgtgcgaa tagagaatgg cgccatcgct gcggtggatt
     1621 gtgggacagc gccagaggtt ctgccggagg aacaggtgat cgatgcggcc ggcgcggtgc
     1681 tcatgcccgg cctagtgaac gcccacacgc atctttacca agtgttgctt cgcgccgttt
     1741 gggaagacct cgaactgctg ccttggctcc gccgaatcta cggctgtgcc cgtgtccttc
     1801 ggcccgagca tttttacgcg ggaagcttgc tcggatgtgt cgaggcgctc cgcagcggag
     1861 tgacgaccat ttgtgagcac aattttttga atccgagccc cgactgcgcc ttcgagacaa
     1921 ttcgggcgat cgaggactcg ggcgtgcgag gagtcttcgc acgcacgatc atggatacgg
     1981 gcgaaatcgt gcccgattgc acgaaggaaa agcccgaggc agcatttcga gagatcgaga
     2041 atattctgag tcaacatcag aatcgagacc gtcttagctt tatgaccggg ccgaatactc
     2101 cgccgatcaa cacgacgcct gagttgctcc gagagctccg gcaatttgcc gatgatcatt
     2161 tgctgggcat cagcgctcat gtcgcagaat cgaaatcagt cgtgcagtcc gtaaaaaatc
     2221 aatacggaaa aaacggagtg gtcgagttcc ttgctcagtt tggcattccg gcggccaatt
     2281 caatattcgc tcattccgtg cacgtctcac aaagagaaat cgatgttctc cgggaaaccg
     2341 gcacgtcggt ttctcacaat ccggttagca acatgatgct tggagacgga attgcgccgg
     2401 ttgtcgaaat gcttcggcag ggggtgaaca tcagtctcgg tacggacggg gcggcgagca
     2461 accattcaca agatcttttt gaaacgatga aggcggcttc gttgctgcaa aaggtgcatc
     2521 atcaagacgc cggcgtcatc gatccgtttt ccgtgctgcg tatggccacg gccggcggcg
     2581 ccaaagcgct tggcctcgac tctgtttgcg gaaccgtgga agtcgacaaa cgagcggatt
     2641 tgatgcttgt cgatatcgat acagttcaca atcaaccggt gaacgacatc tttagccaac
     2701 tcgttcattg cgccaaggct agcgatgtac acacggtgat aattgacggc gaaatattga
     2761 tgcgcgacca gcaatttcac cggatcgatg agaggcgggt tctagctgat gcaaaggtgg
     2821 cgcaccgaga tttgatggag cgggtgagcc aagtgtcttt ttaaaatatg ctccggcgat
     2881 tatggacgcc ggttacggct taatgtcctg cataaaccat tcgctgggga tttctcgtcc
     2941 caagcctttt tccctcgcgc ctttatatgc gcagtaaccc atggccgcga actgcactcc
     3001 ggtgccgaca ttattcaaga aaaaagtgat ttgctgatca tcggttctgc tcggtgcttt
     3061 gccggcaatg agatcacaaa tttccggata ctttgaccag tcacgcggct tttcgcggcg
     3121 cactccgggc atgtcatcca ggattcccgg cggctggtag tgctgtatac cgccgaggtt
     3181 tgtgtggatc gcaactactt cgcaccgttc caaaaccgac agatcggtct caaattccct
     3241 aatggcattg taatgcatcc caggcttgag ccagtcgatg ttcaaaactg gctgtgaagc
     3301 attggtcgcc gtgcaaacga catccacgtc ggtagccgct tgttcgactg tatcaacggc
     3361 ttcgcccgcc acgccggttt cctttgtcat ctcctcgacg aactttgttc ggtgcccggg
     3421 attgggactg tagaccttga cctttttgag cgccatcaca gccgtcatga aacggtagtg
     3481 ggctttggcc tgtccgccag agccgatcac gcccaacacc tgtgaatttt tccgcgccat
     3541 gactctcata gctaatgcac tggtgacacc gacacgagtc tgctgaatga gaccgtccgg
     3601 catgatcgcc agaagctcgc cggtttcggc gctgaataat tgcaccaacc caacaaacat
     3661 accgtggccc gcaaggggca atttttccaa tcgcatcgag ccgtgcacca cctgctcttg
     3721 aacgatatcc gaggtgaccc gcaaagctag cacaccgtat ttgccgatgg cgccatcgac
     3781 ggatttgaag ctataagtcg cgttcggaag cgaatgcggt aagtaagtgt ggcaggtcgg
     3841 ccgattggca gcgcggctag ccgcttgctc gcgatacgac tctgccatta ccgcaaggca
     3901 ttcggcgaca tcaagcgcgt tctggacatc agcgttactt aaaataagcg tcatcaagat
     3961 ctccgaagcg aaattggatt tcta
//
LOCUS       DASULZ010000684         4679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_179982, whole genome shotgun sequence.
ACCESSION   DASULZ010000684 DASULZ010000000
VERSION     DASULZ010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179982"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..338)
                     /locus_tag="VFY96_14165"
     CDS             complement(165..338)
                     /locus_tag="VFY96_14165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX5607665.1"
                     /translation="MPTYEFLCQKCKKRFEVIWSLVQYDREMKRKPKCPKCSSTSVVR
                     QISSVEVKTSKKS"
     gene            859..1014
                     /locus_tag="VFY96_14170"
     CDS             859..1014
                     /locus_tag="VFY96_14170"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5607666.1"
                     /translation="MKVEEIMIREVAQSSAENSINEAAKLMRGKRVGCLVITADGAII
                     KGPYDGS"
     gene            1073..1420
                     /locus_tag="VFY96_14175"
     CDS             1073..1420
                     /locus_tag="VFY96_14175"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5607667.1"
                     /translation="MSRPVAVIKPEEHYLTAADVMRRRRIKRLPVAKQGKLLGIVSLS
                     DLAKLAVKDVGQIKSSLGLLSSFLSAVEIQSRTNRRTRPEIEPSTRADGTQTASRKTS
                     PSEINQEEQVAQL"
     gene            1417..2766
                     /locus_tag="VFY96_14180"
     CDS             1417..2766
                     /locus_tag="VFY96_14180"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017841046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX5607668.1"
                     /translation="MIAESSGQTNILLTDLYQLTMLQGYFDGGMEETAVFEFFVRKLP
                     EQRNFLIAAGLEQALGFLENARFTSKDLEWVSRQPAFRREFVEQLVKWRFTGDADAMP
                     EGTIFFPNEPILRVTAPLPQAQLVESRLINLIHFQTLIASKAARSVLLAPDKLLVDFG
                     LRRAHGAEAGLLAARASYLAGFSGSATVLAEAAYGVPVYGTMAHSFIQAHEHEETAFD
                     HFAHSLPGNVILLIDTYDTEAAAEKVVQLAAKLERDGISIKGVRLDSGDLADHAFKVR
                     RILDAGGLQYVTIFASGSVDEYVIKRLAEKNAPIDGFGIGTHMDTSADAPYLDCAYKL
                     VEHAGKARRKRSEGKVLWPGRKQVFRSYDHAGHISGDVLGLESDRLAGERLIQPVMKQ
                     GRRIGTGVSLRQSREKAVEQLRRLPNGLRGIEPASPYPVTVADSVRELARALDAQGD"
     gene            2812..3465
                     /locus_tag="VFY96_14185"
     CDS             2812..3465
                     /locus_tag="VFY96_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX5607669.1"
                     /translation="MRFQNREHAAELLVERLRANYKNQNPVILGIPRGAVPMAKIIAD
                     GLGGELDVVLVHKLSHPDQPELAIGAIDEEGNAFVSEWAADLDPEFLEAEKRRQLEVL
                     RQRRAKYTPLRAPIDPRDRIVIVVDDGIATGSTMTAALRAVRAKKPKKLVGAVAVAST
                     EAARAMLRECDAMVCLSIPAHFYAVGQFFDEFGQVTDEEVIASLHETQQKIVANSNR"
     gene            3970..4203
                     /locus_tag="VFY96_14190"
     CDS             3970..4203
                     /locus_tag="VFY96_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607670.1"
                     /translation="MELIYKSRRIHSLPELNLEMDCWVPNADVLWEQEGTKFHQLLTG
                     PVGYFKIIDQAEIYAVEMAKTWIDAESAEDLTV"
     gene            4232..4669
                     /locus_tag="VFY96_14195"
     CDS             4232..4669
                     /locus_tag="VFY96_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5607671.1"
                     /translation="MYKHILIPTDGSELSARAINEGVALAKALHAEVTAITVSETFHT
                     FSLNPMVVTETPEQYQKDSDAGAEKYLSVANDAAKAAGVPYEGVHVMHDHPYEAIIAA
                     AKERGCDLIVMASHGRKGMSALVLGSVTVKVLTHSNIPALVCR"
BASE COUNT         1132 a         1167 c         1294 g         1086 t
ORIGIN      
        1 acgagcgcgc cctcgagggt tatcccgttt accgggatcg ggacgatacg ctcgagccgc
       61 tcttgctcag gtctttcttt agccatattt caactcccac gctcgcatat ttatttttta
      121 aaactcggag tattgcacac caacgaatct ttaacgtcca tttctcaact ctttttcgat
      181 gtcttcactt caacacttga aatctgccga acgacactgg tgctgctgca ttttgggcat
      241 ttcggtttcc ttttcatctc gcggtcatat tgcaccaatg accagattac ctcgaatcgc
      301 ttcttacatt tctgacatag gaactcgtaa gtcggcacgt tccacctccg ttgctagatt
      361 gtaatgccgt tttcgccgcg acaaagctag tgctcgaagc agcaaccaaa ttttggtcac
      421 gctgatcgac tcaatgagcg agtgaagagg cgttgggctt tggcttaatc catttgagaa
      481 ttaaatcaac gatcagcgtc agcataatga gcagtaccag acagcagctc agtttcttgt
      541 ggcatggcca actccttcga agccgcgcgt ccgctgcggc ctatacttcc ttttccagcg
      601 tgatgcgcct tttgctcaaa ctttaggcgc aagtcatgtg ccagcaaagc cggctttttt
      661 aaaacgtctc tgagttatac atcagcgggg agggtcgacg gaagcatcgc aatcgatttt
      721 ttcaaagttg cttttttcag ttgctagctt tctcatcggc gcgaaaacgc ctgctacttt
      781 tgagcggcca ggcacacgta acggagccaa cttggcatgc gccatgcgtt tcagaactcg
      841 tactagagga gaagccgaat gaaagtcgaa gagattatga ttcgtgaggt cgcccaaagc
      901 tccgcagaaa actccatcaa cgaagcagcc aaactgatgc gtggtaaacg ggtgggttgt
      961 ctggtgataa cagctgacgg cgcgatcatc aaaggccctt atgacggatc gtgacttact
     1021 gggctgtatt gcagctggac atgatccacg cgaatgcaag atctccgcgc atatgagccg
     1081 gcctgtggct gtgatcaaac cagaagagca ctacttaact gccgccgatg tgatgcgccg
     1141 gcgacgaatc aagcggttgc cggtcgccaa acaaggcaag ctgctcggaa tcgtatctct
     1201 atcggactta gccaagcttg cagtaaagga cgtggggcag atcaaatcct ctctagggct
     1261 tctttcgtcg ttccttagcg ctgtggaaat acaatcgcgc accaatcgtc ggacccgacc
     1321 agagattgag ccgtccacca gagccgatgg cacccagacg gcgagtcgga agaccagccc
     1381 ttcagaaatc aatcaagagg agcaagtggc gcagttatga ttgccgagag cagcgggcaa
     1441 acaaacatac ttctgaccga tctttatcag ctgacgatgc tccaaggcta cttcgatggc
     1501 ggaatggagg agactgctgt attcgagttt tttgtgcgca agctgccgga gcaacgcaat
     1561 tttctgattg ctgccggatt ggaacaggcg ctcggctttc tcgagaatgc acgctttacg
     1621 tctaaagatt tggaatgggt ctcacgccag ccggcattcc gtcgggaatt tgtcgagcag
     1681 cttgtgaaat ggcgcttcac cggcgatgcc gatgcgatgc cggagggaac aatattcttt
     1741 ccaaacgagc ccatactgcg cgtaacggca ccgctgccgc aagcccaatt ggtggagagc
     1801 cgcctcatca acttgatcca ttttcaaact ttgattgctt cgaaagccgc tcgttcggtt
     1861 ttactcgcgc cggacaagct gttggtcgac ttcggcttgc gccgcgctca tggcgcggag
     1921 gcgggcttat tggccgcgcg cgccagttac ttagcgggct tttccggcag cgcaacggtc
     1981 ttggccgagg cggcgtacgg agtgccagtg tatggcacga tggcgcactc gtttatccag
     2041 gcgcacgagc atgaggagac ggccttcgat catttcgctc actcattgcc tggaaatgta
     2101 attctcttga tcgataccta cgataccgag gcggcggcgg agaaagtcgt tcagctggcg
     2161 gcaaagctcg agcgtgatgg gatcagcatc aagggcgtgc gtctcgacag cggcgatttg
     2221 gcggatcatg cgttcaaagt acggcgcatt ctggatgccg gaggacttca atacgtaacg
     2281 atattcgcca gcggcagcgt cgatgaatac gtcatcaagc gattggcaga gaagaatgca
     2341 cccatcgacg gcttcgggat cggcacgcat atggacacgt ccgccgatgc gccttatttg
     2401 gactgcgcct ataaactcgt tgagcatgcc ggcaaggcca gacgcaagcg ttctgaggga
     2461 aaggtgctct ggccgggtcg aaaacaggtt tttcgtagtt acgaccacgc cggtcatatc
     2521 tccggcgatg ttctgggatt ggaaagcgat cgattggcgg gggagcgatt aattcagcct
     2581 gtgatgaaac agggacgccg gattggtacc ggcgtatctt tgcggcaatc gagggaaaag
     2641 gctgtagaac agctcagacg attgccgaac ggtctcaggg gtatcgagcc agcatcgcca
     2701 tatcccgtga cagttgcaga ttcggtgcgc gagttagccc gcgcactaga tgcgcaggga
     2761 gattagacaa ggtgactgtg gtgccatgcg catagaacct aaggagaagc catgagattt
     2821 caaaaccgcg aacatgcagc agagttactg gtcgaaaggt tgagagcgaa ttacaagaat
     2881 caaaatccgg taattttggg gattccgcgc ggtgcggtgc ccatggcaaa gattatcgcc
     2941 gacggtctag gtggtgagct cgatgttgtc ttggtgcaca agctcagcca tcccgaccag
     3001 cctgagctcg cgattggtgc catcgacgag gagggtaacg ccttcgtgag cgagtgggcc
     3061 gcggacttgg atcctgagtt cctcgaagca gagaagcgcc gccagctcga ggtgctgcgt
     3121 caacgacgcg caaagtatac tccattgcgc gcaccgatcg atccgcgcga tcgcatcgta
     3181 attgtggttg atgacgggat cgcaaccggc tcgaccatga ccgcagcatt gcgggctgtg
     3241 cgcgccaaga aaccaaaaaa actggtcggc gccgtagccg tggcatccac cgaagcagcg
     3301 cgggcgatgt tacgtgaatg tgatgcaatg gtttgtttga gtataccggc tcatttttat
     3361 gccgtggggc agttctttga tgagttcggc caggttaccg acgaagaggt gattgcgagc
     3421 ttgcacgaaa ctcagcaaaa gatcgtcgct aactcaaaca ggtgactctg tttgagcctg
     3481 cgacggatac gtgctgccca agtcacggca ctccggatcc gctgaaccag cttcttacct
     3541 gccaaattct tttttgcggc tttgaataaa aatgtagata tgattcttga atatgacgag
     3601 atcaatcgat ttacggctga aaacaccatt taaaccgggc acaggcattg cgttacggaa
     3661 tttggcaaag gagagagcaa tgacgaccaa aatgaaaacg aaaagactcc gtaccaccat
     3721 gggtgaacta attacggcaa tcatggatgc aactcgaata gtgacaactg acgagcagca
     3781 tgcagatcgt ctcactggtt tcgttttgaa caacgtgttg cggcctgtcc ctgtgacggt
     3841 ggctaatcga agtattagaa gacgcaaagt ttaatctctg cggatggaac atccgcaggt
     3901 cgtccgattt tcgcgccacg acagtaggca tttaacctgc tgtttggaat atagcggagg
     3961 gatcagacca tggaactaat atataagtcc cgccggattc atagcctacc tgagttgaat
     4021 ctcgaaatgg actgctgggt tccaaatgcc gatgtgttat gggagcagga aggtaccaaa
     4081 tttcatcaac tgctcacagg tcccgttggt tactttaaaa ttattgatca agctgagatc
     4141 tatgcagtag aaatggccaa gacgtggatc gatgcggaat ccgccgagga tctgactgtc
     4201 tagcagccca gcgagcagga gagaaaaagc tatgtacaag cacattttaa ttcccaccga
     4261 cggttccgaa ctttcggcaa gagccatcaa cgaaggtgtg gcattagcga aggcacttca
     4321 cgcggaggtg acagcgatca ctgtctctga gacgtttcat actttctctc ttaatccaat
     4381 ggttgttact gagacgccgg agcagtacca gaaagactcc gatgcggggg cggaaaagta
     4441 tctgagtgta gcgaacgatg ctgccaaggc tgccggcgtg ccctatgaag gtgtacatgt
     4501 gatgcatgat catccctatg aagcaatcat tgccgctgcg aaggagaggg gttgcgatct
     4561 cattgtcatg gcctcgcatg gtcgtaaagg gatgtccgca ctagtccttg ggagcgtgac
     4621 agtaaaagtg ttgactcaca gtaacattcc ggctctggtg tgccgctgac ggtacaatt
//
LOCUS       DASULZ010000685         3188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1499504, whole genome shotgun sequence.
ACCESSION   DASULZ010000685 DASULZ010000000
VERSION     DASULZ010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499504"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VFY96_14200"
     CDS             complement(<1..659)
                     /locus_tag="VFY96_14200"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5607672.1"
                     /translation="MAKQGYKILDSDMHVFEPHDLYLRYMNPKWGDRIPRGQPRTTHG
                     QIKFTFADGRPIRSSGTQALAATSPKKNSEESSPGEVEVAQRYGKPLARRYDALSQLE
                     AMDEEGLDVAVLFRTFPLHCDDSLEAEYANDLCKAWNNWMADFCQADPGRLRPSALIT
                     LHDVDMAVDETRRAITELKAIGLCLVPEPVNERHIHDRYYDPLWREAEKLRIPICFHP
                     AA"
     gene            914..2437
                     /locus_tag="VFY96_14205"
     CDS             914..2437
                     /locus_tag="VFY96_14205"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607673.1"
                     /translation="MISARRLAITLTAVTCILCGAFGAQAQSRVSIGVTETIETHNPY
                     GDSVSLQYGIYSEVTGPFCTYSYNKADFEGRLAERWKVENPTTWIFYLSKNYKFNDGT
                     TVTAEDVVHSLMNRVIGDPQSKQKASVAPSVVKAEAVDKFTVKVTTDKPTAPLLSFLC
                     DRLIVTSKVAFDKYGRDAADREHMMGGGPYQLKELIPGQRLVLQKRPDHPDAKKHPNA
                     PDEVIYRIMREPEQRVTAFLNDEIQIAQFIPPHMRQRVEKNPKLRIAPIDSVEIMFLA
                     MQPKPPFDKKEVRQAVCHAINRDQIIATLLEGFASRLDGPIGPGQYGYDPNLKPKMNY
                     DPEKSKKLLAQSGYANGVDVELQTPVGRYTLDKQLTEAMIPMLNAAGFRTRLLTPEWA
                     TLWANVQKGTVPFYYMGRGSVQDPSAALHQYFHTGETPRIGYSNAKLDELLDKEQQEF
                     DPKKRRQYLSQAMSILTDDAPACFLWRHKLLWGLNSRIDYKPLPDARIYAIDMAVKR"
     gene            2529..>3188
                     /locus_tag="VFY96_14210"
     CDS             2529..>3188
                     /locus_tag="VFY96_14210"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5607674.1"
                     /translation="MTSYLVHRLAGTLPVLLLISFLVFSLIHAAPGDPTLLLLGEETN
                     AAEIARAKERWGLDQPLYVQYWKFLSAAAAGDFGKSFKYAEPVINVIKARLPATIELA
                     VFAIIIASLLAIPLGVWAGAKPNSWIDNLGTTFGLFGISMPSFWLAIMLILLLAGVLN
                     VLPTSGRSTYGVAGPEQTGFYLLDSILQKNWAAVWDGLTHIFLPALALGMNMLGILMR
                     VT"
BASE COUNT          709 a          925 c          841 g          713 t
ORIGIN      
        1 gccgccggat gaaaacagat cgggatccgc aatttttcgg cttcccgcca gagcggatca
       61 tagtaccggt catggatatg ccgttcgttg acgggctctg ggacgaggca caggccaatt
      121 gcctttagct ccgtaattgc ccggcgagtc tcatcgacag ccatatcgac atcgtgcagc
      181 gtaattaatg cagacggccg tagtcgtcct gggtccgctt ggcaaaaatc cgccatccaa
      241 ttattccaag ccttacataa gtcattggcg tattcggctt cgaggctatc atcgcagtgc
      301 agcgggaaag tccgaaacaa caccgccacg tccaaaccct cttcgtccat tgcctccagt
      361 tgcgagagcg cgtcgtagcg gcgcgccagc ggcttgccat aacgctgcgc cacctcgact
      421 tcgccggggg aactctcctc ggagttcttt tttggcgacg tggcagccaa tgcctgggta
      481 ccggaagatc ggattggccg tccatcggca aaggtaaact tgatctggcc atgagttgtc
      541 ctcggttgcc ctcgcggaat ccgatcgccc catttcggat tcatatatct aaggtagaga
      601 tcgtgcggct cgaacacgtg catgtcgctg tcgagaattt tgtaaccttg tttcgccacc
      661 gtgtcctcct cttgctacgg acgaaagcgc actgccgaag cgaagcatct caggatagca
      721 atccgactcg gtcctaaatc gcgcgcaata atgccggcgc tctcaatcaa gcattagtac
      781 cggagcttta cttctgtcaa cggcacactg catagccgat ggcaccagaa gttttccgcc
      841 ttgacaagcc aggccgctgc tgtaaatgat ttcgcatcga tcgtccgagc atctcgtttt
      901 ttggaggttc atcatgatca gcgcacgccg tcttgcgatt acccttacgg cagtgacttg
      961 catcttgtgc ggcgccttcg gggcgcaggc gcagagccgg gtttccattg gtgtcaccga
     1021 aaccatcgaa acccataacc cttacggcga tagtgtttca ctgcagtatg gaatttacag
     1081 cgaagtcacc ggtccgttct gcacttacag ctataacaaa gctgattttg aggggcgtct
     1141 cgccgagcgc tggaaagtgg aaaatccgac gacctggatt ttctacttga gcaagaacta
     1201 caaattcaac gatggcacga cggtcaccgc tgaagacgtg gttcactcgt taatgaatcg
     1261 ggtcatcggc gatccccaat cgaaacaaaa agcctccgtc gcgccctcag tcgtcaaagc
     1321 cgaagccgtg gacaaattta ccgtcaaagt caccacggac aaaccaacgg caccgctact
     1381 gtcgtttctt tgcgatcgac tgattgtgac cagcaaagtt gctttcgaca agtacgggcg
     1441 cgatgccgcc gatcgagagc acatgatggg cggcggtcct tatcagctga aagagctcat
     1501 tcccggtcag cgcctggtgc tgcaaaaacg gcccgaccat cccgatgcga agaagcatcc
     1561 caatgcgcct gatgaagtca tctaccgcat catgcgcgag cctgaacaac gggtgacagc
     1621 gttcctcaac gacgaaattc aaatcgcgca attcatcccg ccccacatgc gccagcgcgt
     1681 tgaaaaaaat cccaagttgc gcatcgcgcc gatcgattcc gtggagatca tgtttctcgc
     1741 tatgcaaccc aagccgcctt tcgacaaaaa agaagtccga caggcggtgt gccacgcgat
     1801 taaccgcgat cagatcatcg ccacgcttct cgaagggttt gccagccgtc tggatggtcc
     1861 cattggcccc gggcagtacg gctacgatcc caatttaaag cccaagatga actacgatcc
     1921 ggagaaatca aaaaaattgc tcgcccaatc gggttacgcc aatggtgtcg acgtcgaatt
     1981 gcaaaccccg gtgggccgtt acacgctgga caagcagttg accgaagcga tgattccgat
     2041 gctcaacgcg gcgggatttc gcacccggct gctcacgccg gaatgggcga cgctctgggc
     2101 aaatgtgcaa aagggcaccg ttccttttta ttacatgggc cgcggctccg tgcaggatcc
     2161 gagcgccgca ttgcaccaat acttccatac gggcgagaca ccgcggattg gctattccaa
     2221 tgccaagctg gacgagctgc tcgacaagga gcaacaagag ttcgatccga aaaagcggcg
     2281 ccagtacctt tcgcaagcga tgagtattct gaccgacgat gctccggcct gttttctctg
     2341 gcgccacaag cttctttggg gactcaacag ccgcattgat tataagccac tgccggacgc
     2401 gcgcatttat gcgatcgaca tggcggtaaa gcgctaaacc agtttggacg gtggcgtaat
     2461 ggagtggtgg agagatggtt agacccaata cagaagtact ccaacactcc acgacctgat
     2521 ttctcgttat gactagctat ctcgtccacc gtctcgcggg cactctgccg gttctgttgc
     2581 tgattagctt cctggttttt tctttgatcc atgccgcgcc cggcgatccg acattgctgc
     2641 tacttggcga ggaaaccaac gccgcggaaa ttgccagggc aaaagagcgc tggggcttgg
     2701 atcagcctct ctatgttcag tattggaaat ttctgagcgc cgccgcggcc ggcgatttcg
     2761 gcaagtcgtt taagtatgcc gagccggtca tcaatgttat caaggcgcgc cttccggcga
     2821 ccattgagct cgccgtgttc gcaatcatca tcgccagcct gctcgccatc ccgctcggcg
     2881 tctgggccgg cgccaagccc aattcatgga tcgataatct cggcaccact ttcggtcttt
     2941 ttggtatcag catgccgagt ttctggctcg cgattatgtt gatccttttg ctggctggcg
     3001 tgctcaatgt gctaccgaca tccggacgga gcacctatgg cgtcgccgga ccggaacaga
     3061 ccggcttcta tcttttggat agcatcctgc aaaaaaactg ggcggcggtc tgggacggac
     3121 taacgcacat ttttctgccg gccttggccc tcggcatgaa tatgctgggc attctgatgc
     3181 gcgtcacc
//
LOCUS       DASULZ010000686         7775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_891051, whole genome shotgun sequence.
ACCESSION   DASULZ010000686 DASULZ010000000
VERSION     DASULZ010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7775
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891051"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VFY96_14215"
     CDS             complement(<1..426)
                     /locus_tag="VFY96_14215"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607675.1"
                     /translation="MINSHVILSCRLAVTLIVSMIFVAPAQAAVRISMTGFAGQFMTF
                     PLAQKRGFLKEEGFEAEVLRIAAAAGRAALTNGDIDYSTGIGETAIGGALSGIPIRVV
                     ACFVPAPVLALVARPEFKTIPSLKGKTVAILIAGGVAHFA"
     gene            complement(535..1524)
                     /locus_tag="VFY96_14220"
     CDS             complement(535..1524)
                     /locus_tag="VFY96_14220"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607676.1"
                     /translation="MKQFIMALTLFLSLCFSVAFPQTTQLTFGYSSTASAELPAWLAK
                     ETGIFARNGLDVQLVYFRGGTIATMALLSRQTPISQGSGQASVNAALRGADTVMVAGG
                     LVDTEWWLLTRSDIKTAEQLKGGTLAISPFGGLAESMTRIALKRLGLTPVKDVAMVQV
                     NGLQERLFALETGKVQGAMLPTPYKFIGQKRGFHNLLSVSLPYQSTGVSTTRTIIREN
                     PDLVRKYVKSQIEAIHRIKTDRETAVKLLVKYLGPQDNDIFAKSYDELSSDEKFPPKQ
                     YPTLEGIRNILEPLAQTDPKAKAARPEDFVDMRFVKELEESGFVAELYKSRKR"
     gene            complement(1577..2500)
                     /locus_tag="VFY96_14225"
     CDS             complement(1577..2500)
                     /locus_tag="VFY96_14225"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607677.1"
                     /translation="MVIFLQTAVHGADKIRIGYPDFNSSTFTLPLAQMRGFFQEEDLE
                     AELIRVRSAVAIPALTSGEIDYHTVVSPALAAALRGIPVRLVACFTPGLTTVIIARPE
                     FKRIQDLKGKTIGLNSIGGGLESTVRMMLKHYGIDPDKDVKFLATGTVDTRFAALKQG
                     LTVATAGSTPVDYLGKKLGYVVLARAYDLFSYPNTGLVASVKKIKENPDQLKRVIKAG
                     IKANRYLHQNRDGTIQAIMQWMKVDKETAAATYDGAAKSFNEDGSVPEAGLRLAIDEA
                     RKAAGISREVAPSEVADFTVLRDAQRESGSK"
     gene            complement(2685..2915)
                     /locus_tag="VFY96_14230"
     CDS             complement(2685..2915)
                     /locus_tag="VFY96_14230"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5607678.1"
                     /translation="MACGTGIVARLTADRVAPSGSVVGLDANEAMLTVARAQPAPTGA
                     QLVEKMTPELLKQTKSEVFRKLQTLNKPRWFP"
     gene            complement(3444..4439)
                     /locus_tag="VFY96_14235"
     CDS             complement(3444..4439)
                     /locus_tag="VFY96_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HEX5607679.1"
                     /translation="MDLSTSYLGLRLPHPLVPGASPLSDDLDTVRQLEDAGAAAIVLR
                     SLFEEQITREAQATHDHWNSHDNAFAEAITFFPSADSFVFGPDEYLNHLRRVKQTVQV
                     PVIGSLNGMTPGGWLSYARLIEQAGADALELNVYHAPTDFETSGSEVECQTLEMVRQV
                     KHGLKIPLAVKLSPYFTAFSHFARQLDGAGVDGLVLFNRYYDPDIDVQELNVLRTLQL
                     SDSSELPLRLRGIAALAGRVKASLAVTGGVHTALDVVKSTMAGADITQVVSALLRNGP
                     GHLKKIRTDLEAWMEENEWSSLNEMRGNMSQERIPNPQIYERANYMLMLQTWERT"
     gene            complement(4449..>7775)
                     /gene="nifJ"
                     /locus_tag="VFY96_14240"
     CDS             complement(4449..>7775)
                     /gene="nifJ"
                     /locus_tag="VFY96_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEX5607680.1"
                     /translation="IPNMYKIAGELTPFTMHVAARSIATHALSIFGDHSDVMACRQTG
                     FAMLCSGSVQEAHDFAAIAHAATLESRIPFLHFFDGFRTSHEIAKIEELKDDDLRILI
                     SENKIRQHREHGLTPDRPVLRGTAQNPDVFFQARESANRFYDACPAIVENMMERFANL
                     TGRRYSLFDYVGDSQAERLIVIMGSGAETVDETVQYLVERGEKVGVIKVRLFRPFSGT
                     ALLATLPSSIRSIAVLDRTKEPGAVGDPLYLDVVSTLAEARADGKLPFASEPRIVAGR
                     YGLSSKEFTPAMVKAIFDELAKERPHNHFTIGIVDDVTHTSLTWDPSIRTEAEDVDTA
                     VFYGLGADGTVGANKNSIKIIGEKTDRYIQGYFVYDSKKSGAITISHLRASPHPIRSA
                     YLITSAKFVACHQFDFVDKIDVLEHAAAGAVFLLNVPGAPHGVWERLPCELQQQMIEK
                     TIRFFAIDAYAVAKETGMGSRINTIMQTCYFAISGVLPREEAIAKIKKAIEKTYDKMG
                     ADTVRRNFEAVDQTLAHLHEIPLPHAVSATRNRPPLVSEKAPDFVQKVTAVMMAGKGD
                     LLPVSAFPVDGTWPVATGKWEKRAIALEIPVWDTTICIQCNMCALVCPHAAIRAKVYE
                     PAERENAPATFKSTPYKGLDFRNKDYTIQVAPEDCTGCNLCVNVCPAKDRTNPRHKAI
                     DMQPMAPLREAERENYSFFLNLPETERSEVTRLDHKGSQFLEPLFEYSGACAGCGETP
                     YIKLLTQLFGDRLLIANATGCTSIYGGNLPTTPYTTNREGRGPAWSNSLFEDNAEFGF
                     GFRLGVDSHMAAARALVERLGTALGDILVKELLGADQTSESGIAAQRERVKALRRMLS
                     AMDTPEARRLETLADYLVTKSIWLLGGDGWAYDIGYGGLDHVLASDRNVNVLVLDTEV
                     YSNTGGQQSKATPLGAVAKFASAGKSTAKKDLGLLANSYGHVYVARVAMGAKMPQTVQ
                     AFLEAEAYPGPSLIIAYSHCIAHGYDMAHGMSQQKLAVDSGVWPLYRFDPRRLAKSEP
                     PLHLDYGAPKARVADYMRNEARFRVVERTNPARFRGFLKQAQEAAQKRHAVYEQLARI
                     TVPQVVRSKEEDSDGSKPAK"
BASE COUNT         1581 a         2247 c         2234 g         1713 t
ORIGIN      
        1 ggcaaagtgg gcaaccccgc cggcgattag aatcgccacc gtttttcctt tgagcgacgg
       61 gatggtttta aattccggcc gggcgaccag tgccaagacc ggcgcgggca cgaagcaggc
      121 cacgactcgg atcggaattc ccgagagcgc gccgccgatc gccgtttcgc cgatgcccgt
      181 ggagtaatcg atatcgccgt tcgtcaacgc ggcgcggccc gccgctgctg cgatgcgcag
      241 aacttccgct tcgaaccctt cttctttgag aaagccgcgt ttctgcgcga gaggaaaagt
      301 catgaattgt ccggcaaagc cggtcatgga gatgcggacc gcggcctgag ccggcgcgac
      361 aaagatcata gagacgatta aagtgacggc gagtctgcag ctcagaatga catgagaatt
      421 gatcacggtt tttctgatat ccgaggagcg aagcgacgaa gaatctcagg ccgaaccact
      481 acccacaaac ttcgcccaac aaatcgcgcg ctagtcggta ccaaagcgca tcgcctaacg
      541 ctttctgctc ttgtataact cagcaacaaa cccgctttcc tcgagttctt taacgaatct
      601 catatcgacg aagtcctctg gcctcgctgc ttttgctttc gggtccgttt gcgccagcgg
      661 ctctaaaata tttctgatcc cctccagggt cggatactgt ttaggcggaa atttctcgtc
      721 cgagctgagc tcgtcatagc tctttgcgaa gatatcgtta tcctgcgggc cgagatattt
      781 caccaggagt tttaccgctg tttcgcgatc ggttttgatg cgatgaatcg cctcgatctg
      841 cgacttgaca tatttacgga cgaggtctgg attctcacgg atgatagtgc gcgtcgtgct
      901 gacccctgtg ctctgatacg gcagagacac gctcagcagg ttgtgaaagc cgcgtttctg
      961 accgatgaat ttatagggcg ttggaagcat ggctccctgg actttgccgg tctccaaggc
     1021 gaacagacgc tcttgcagtc cgttaacctg taccattgcg acatctttca ccggggtgag
     1081 gccgagcctc ttaagcgcga tgcgggtcat ggattccgcc aatccaccaa aaggactgat
     1141 cgcaagcgtg ccgcccttga gctgttcggc ggttttgata tcggaccgag tcaaaagcca
     1201 ccactcggtg tctaccagtc caccagcgac cataacagtg tcggcgcctc tgagcgcggc
     1261 attgacactc gcttggcccg acccctggct gatcggcgtt tgccgggaga gcaatgccat
     1321 cgtcgcgatc gttccgcctc tgaagtagac aagctgaacg tccaagccgt tccttgcaaa
     1381 gatccccgtc tctttagcca gccaggcggg gagttccgct gaagccgttg aactgtatcc
     1441 gaaggtcagt tgggtggtct gcgggaacgc gacagagaag caaagtgaca agaacagtgt
     1501 cagcgccatg atgaattgct tcatggaagc ggcctctgtt tagaaccggg cgcggacagc
     1561 cttcgtgaaa cacacatcac ttactccccg attcccgctg agcgtcgcgc agcaccgtga
     1621 agtcggctac ttcgctcgga gccacttccc ggctgatacc cgccgccttt ctcgcttcat
     1681 cgatggccag ccgcaaaccc gcttcgggga cgctgccgtc ctcgttgaac gactttgctg
     1741 caccgtcata ggtcgccgct gcagtttctt tatcgacttt catccactgc atgatggctt
     1801 gaatggtgcc gtcgcgattc tgatgcagat agcggttcgc cttgatgccg gctttgataa
     1861 cgcgcttcaa ttggtcggga ttttccttga tctttttgac gctcgcgacc aaacccgtgt
     1921 tcggataact gaacaagtcg taagcccgcg ccagcaccac gtatcccaat ttctttccga
     1981 gataatccac cggagtggat ccggccgtgg ccacggtcaa tccctgcttt agggcagcaa
     2041 agcgcgtatc gacggttccc gtcgcgagga acttcacatc tttgtcggga tcgataccgt
     2101 aatgcttcaa catcatgcgc accgtgctct cgaggccgcc gccgatgctg ttgagaccga
     2161 tcgtcttccc cttgagatct tgaatcctct tgaactccgg acgggcaatg atcacagtag
     2221 tcaaacccgg tgtaaaacag gccaccagcc gcaccggaat accgcgcagc gcggcggcga
     2281 gcgcggggct gaccaccgta tgataatcaa tttcgccgct cgtcaatgcc ggaatggcaa
     2341 cagcggatcg aaccctgatc agctccgctt ccaggtcctc ttcctgaaag aatccccgca
     2401 tctgtgccaa aggcagcgtg aaggtggacg agttgaaatc cggataaccg atgcggattt
     2461 tatcggcacc gtgaaccgct gtctgaagga aaataaccat cgacaaggtc agtaggattt
     2521 ttctgatcct attaaccttt cggtcggcca accggttgtg ttgctgtttg tcaaaactat
     2581 gcgagagttt catcatcaag ctcattgcca atcgcttcga atgcaaagct tgcctcgcgt
     2641 acaatgcgcc ctttgtggcg aaggcgaata aagcgcgact cactttatgg aaaccatctg
     2701 ggcttgttga gcgtttgtaa ttttcgaaat acttcgcttt ttgtttgttt gagcagttcc
     2761 ggcgtcattt tctcgacgag ttgcgccccc gttggcgcgg gctgcgcacg ggcaactgtg
     2821 agcatcgcct cattggcgtc taacccaacg acggagccag atggagcaac ccgatcggca
     2881 gtcagacggg caacaatacc agtgccacaa gccacatcca gcgccgcatg ccgggcgcaa
     2941 tgccgctgtt tctaacaagg cctttgccca aacggcgaag acgccaggca ccatgacccg
     3001 ctcgtagccg gcgctataaa actggatttc ttctgcatat tcggtccgcc cgattttcat
     3061 cgtggactgc ttctacaatg ccgatggagt acttgtaaag gcaattgcca cagccggtcc
     3121 aacaaaggcg gaaactgttt ctgtaatctc agaactataa tcgatgcagg accgcccggt
     3181 ctgttcgttt actttgctat tactttgcta gaactatatg acacgatgtg ttcgaatcgg
     3241 taaatcgacg ggctaatagc gccgttaaac gctcgaatag atacacatag cgaccgatag
     3301 tgtcacatag tctcgccccg gcaagaggac tgaaaatcct cgtgtcgggt gcaatggttt
     3361 gtttcaaaaa gaaaattgta aataacgcac tcatggggcg ggtcatgaaa gatctcacgg
     3421 tcagccaggc gatcactggc gccttacgtc cgttcccagg tctgaagcat cagcatgtag
     3481 ttggcgcgct cgtatatctg tgggttgggt atccgctcct ggctcatgtt gccgcgcatc
     3541 tcgttgagtg aggaccattc gttctcctcc atccatgctt caaggtcggt gcgaattttc
     3601 ttgaggtggc cagggccgtt ccgaagcagc gccgaaacca cctgcgtgat gtccgctccc
     3661 gccatcgtgg acttcactac gtccagagca gtgtgaaccc cgccagtaac cgcgagtgag
     3721 gccttaacgc gtcccgcaag cgcggcgatc ccgcgcaaac gcagcggcag ctcggaagag
     3781 tcggaaagtt gcaaagtgcg caagacgttc agctcctgca cgtcgatatc gggatcgtag
     3841 tagcggttga acagcaccag cccgtcgacg ccggcgccat caagttggcg cgcgaagtgg
     3901 gagaaggcag tgaagtatgg tgaaagtttc acagccagcg ggattttcaa tccatgtttc
     3961 acttggcgaa ccatctcgag agtctggcac tcgacctcgc ttccgctcgt ctcaaagtcg
     4021 gtcggagcat ggtagacgtt gagctccaaa gcgtccgctc cggcttgctc gatcagtcgc
     4081 gcgtaggaga gccaaccgcc cggagtcatg ccgttgagcg acccgatcac ggggacctgg
     4141 accgtttgct tcacgcgccg caggtgattg aggtactcgt ccggaccgaa cacaaaggaa
     4201 tcggcgctcg gaaaaaaagt tatggcctcg gcgaaagcat tgtcgtgact gttccagtga
     4261 tcatgggtcg cttgcgcttc gcgcgtgatc tgctcctcga aaagcgagcg taaaacgatc
     4321 gcggcggcac ccgcgtcctc aagctgcctc acggtatcga gatcgtcgct cagcggtgac
     4381 gcgcccggga ctaggggatg cggcagccta agtccgagat aacttgtact aagatccatg
     4441 tgctcctcct actttgccgg cttcgacccg tcgctgtctt cttccttgga tctaacaacc
     4501 tgaggcacgg tgatgcgcgc cagctgctcg tagaccgcgt ggcgcttctg cgcggcctcc
     4561 tgcgcctgct tgagaaaacc tctgaagcgt gcgggattgg tgcgctcgac cacgcggaag
     4621 cgcgcctcgt tgcgcatgta gtcggcgacg cgcgctttcg gtgcaccata gtcgagatgg
     4681 agcggcggtt cgctcttggc taggcggcgt gggtcgaaac ggtatagcgg ccagacgccg
     4741 gaatccaccg cgagtttctg ctgtgacatg ccgtgagcca tatcgtagcc gtgggcgatg
     4801 caatggctat aggcgatgat gagcgaaggc cccggatagg cttcggcctc gagaaatgcc
     4861 tgcacggttt gcggcatttt ggcccccatg gcgacgcggg cgacgtagac atgaccgtag
     4921 ctgttggcca gcagtcccaa atctttcttt gcggtgctct ttcccgccga agcgaatttt
     4981 gcgaccgcgc cgagcggcgt cgccttggat tgttggccgc cggtattgga gtaaacttcg
     5041 gtgtcgagga cgagtacatt gacgttgcgg tcgctggcga ggacatgatc gagcccaccg
     5101 taaccgatgt cgtaggccca accgtcgccg cccagaagcc agatgctttt cgtgacgagg
     5161 taatcggcca gtgtctccag ccgccgcgcc tccggcgtgt ccatagcgga gagcatccgg
     5221 cgcagtgcct tcacgcgttc gcgctgtgcg gcgatcccgg attcactcgt ctggtcggcg
     5281 ccaagcagct ccttcaccag gatatcgccc agtgccgtcc cgagccgttc gactagcgct
     5341 ctagcggccg ccatgtggct atcgactccc aggcgaaagc caaagccgaa ttccgcgttg
     5401 tcttcgaaca gcgaattgga ccaagccggc ccgcgtccct cgcgattcgt tgtgtacggc
     5461 gtggttggca ggttgccgcc gtagatcgac gtgcagccgg tcgcgttggc gatcagaagg
     5521 cgatcgccaa aaagctgggt gagcagttta atgtaaggcg tttcgccgca gccggcgcac
     5581 gcccccgaat attcgaagag cggctcgaga aattgtgagc ccttgtgatc aaggcgcgtc
     5641 acctcgctgc gttcggtctc tggcaagttc aagaagaacg aataattttc ccgttcggct
     5701 tcgcgtagcg gcgccatcgg ctgcatgtcg atcgctttat gccgcgggtt ggtccggtcc
     5761 ttggctgggc agacattgac gcacaaatta cagcccgtgc agtcctccgg cgcgacctgg
     5821 atcgtataat ccttgttcct aaaatcgaga cctttgtagg gcgtcgactt gaatgttgcc
     5881 ggagcgttct cccgctcggc gggttcgtag accttggcgc ggatcgcagc gtgaggacaa
     5941 accagtgcac acatgttgca ctgaatgcaa atcgtcgtgt cccagaccgg aatctcgaga
     6001 gcgatggcgc gcttctccca tttgccggtg gccacaggcc atgtgccgtc gacggggaag
     6061 gcgctcaccg ggagcagatc tcctttgccg gccatcatca ctgcggtgac cttttgcacg
     6121 aagtccggcg ccttttcgga gaccaacgga ggccgattac gggtggcgct caccgcgtgg
     6181 ggcagcggga tctcgtgcag gtgcgcgagt gtctgatcca ccgcctcgaa gttccggcgt
     6241 acagtgtccg ctcccatctt gtcgtaggtc ttctcgatcg ccttcttgat cttggcgatt
     6301 gcctcctcgc gcggcagcac gcccgagatc gcaaagtagc aagtttgcat gatcgtgttg
     6361 atgcggctgc ccatgccagt ttctttagct acagcgtaag cgtcgatggc gaagaagcgg
     6421 atggttttct cgatcatctg ttgttgcagc tcgcacggca agcgctccca cacgccgtgc
     6481 ggggctcccg gaacattcaa taggaacacc gcgccggcag cggcgtgctc gagcacatcg
     6541 atcttgtcga cgaaatcgaa ctgatgacag gcgacaaact tggcgctggt gatgaggtag
     6601 gccgagcgga tcgggtgggg gctggcgcgt aggtgcgaga tcgtgatcgc cccagatttt
     6661 ttagagtcgt aaacaaaata gccctggatg taacggtcgg ttttctcgcc gatgatttta
     6721 atcgagtttt tgttcgctcc caccgtaccg tcggctccga gtccgtaaaa gaccgccgtg
     6781 tcgacatcct cggcctcagt gcgtatcgat ggatcccaag taagcgaggt gtgcgttacg
     6841 tcatcgacaa tcccaatagt gaagtggttg tgcggccgtt ctttcgcaag ctcgtcgaag
     6901 attgccttaa ccatggccgg cgtgaactct ttcgacgaca gcccataacg gcccgcgact
     6961 atacgcggct ccgaggcaaa aggtagtttt ccgtctgcgc gcgcctcggc gagcgtagac
     7021 acgacgtcaa gataaagcgg atcgcctacc gcaccgggct ccttcgtgcg atcgaggacg
     7081 gcgatagaac ggatgctgga cggcagtgtc gcgagcagcg ccgttccgga gaacggccgg
     7141 aatagtctca ccttgatgac gccaactttc tcgcccctct cgacgaggta ctgcacggtc
     7201 tcgtcgaccg tttctgcgcc agagcccatg atgacaatca ggcgctctgc ctgtgagtcc
     7261 ccaacgtaat caaagagact gtagcgccga cccgtcaagt tggcgaaccg ctccatcatg
     7321 ttctctacga tcgccggaca ggcgtcatag aagcggttgg cggactcgcg ggcctggaag
     7381 aacacgtcgg ggttctgcgc ggtgccgcgc agtacgggcc gatccggcgt caatccatgc
     7441 tcccggtgtt ggcgtatttt attttccgag atcaggattc ggaggtcatc atccttcagt
     7501 tcctcgatct tcgcgatctc atgtgacgtg cgaaagccgt caaaaaaatg gaggaacgga
     7561 attctcgact ccagcgtagc ggcgtgggcg atcgccgcga agtcgtgcgc ttcctgcacc
     7621 gaaccggaac agagcatggc gaagccggtt tggcggcaag ccatgacatc ggagtggtcg
     7681 ccgaaaatcg atagcgcatg cgtggcgatg ctgcgcgccg ccacgtgcat ggtgaacggc
     7741 gtcagctccc cggcaatctt gtacatattg ggaat
//
LOCUS       DASULZ010000687         2376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_79794, whole genome shotgun sequence.
ACCESSION   DASULZ010000687 DASULZ010000000
VERSION     DASULZ010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79794"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /gene="cysD"
                     /locus_tag="VFY96_14245"
     CDS             complement(<1..714)
                     /gene="cysD"
                     /locus_tag="VFY96_14245"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEX5607681.1"
                     /translation="MNYDVCSELESESIEIIREVAATARKPVMLYSIGKDSSVMLRLA
                     QKAFYPAPIPFPLMHIDTGWKFREMIAFRDRTAQQLGLQLITFTNPRGAAEGIGPVTH
                     GSAYHTDVMKTEALKHALDLHGFDFVFGGARRDEEKSRAKERIFSFRAVGHRWDPKSQ
                     RPELWNLYNTRINAGETVRVFPLSNWTEGDIWQYIRQENIPIVPLYFAAKRPVVKRNG
                     QWIMVDDHRLPMEPGEKPEL"
     gene            928..1320
                     /locus_tag="VFY96_14250"
     CDS             928..1320
                     /locus_tag="VFY96_14250"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5607682.1"
                     /translation="MLKTHGVLHFSLPVTDLDRSRNFYEQLLGMKVVEQSPRMVFLQT
                     GDDYLILAKGNEPLKYDSSKSTPVHHAFKVKPEDFEPSIEFLRRNGVEVFNIEDRNQG
                     VFWGPQAYFLDPDGNKLEIYAGPGAKNS"
     gene            complement(1481..2287)
                     /locus_tag="VFY96_14255"
     CDS             complement(1481..2287)
                     /locus_tag="VFY96_14255"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5607683.1"
                     /translation="MNRHYFAYNFWAALSILLAAALAQAQPAKEFQPEVGQAGKDVVW
                     VPTPQALVDKMLDMAKVTPKDYVIDLGSGDGRTVITAAKRGSKALGIEYNPEMVELSK
                     RNAAKEGVSDKASFMKADLFETDFSQAQVITMFLLPSINMKLRPKLLNLKPGTRIVSN
                     TFDMEDWKPDEDATVENCTNWCTAHLWIVPAKADGTWKTAQGDLTIKQMFQNITGTLK
                     NGSGVTPINGKLHGSQITFTAGSTQYTGNVNGNSIDGTASGAKWTATRGK"
BASE COUNT          549 a          615 c          647 g          565 t
ORIGIN      
        1 cagttccggc ttttcgccgg gctccatggg caaacggtga tcgtcaacca taatccattg
       61 accgttacgc ttaaccaccg gccgttttgc tgcgaaataa agcggcacaa tcggaatatt
      121 ttcctgtcgg atgtattgcc agatgtcgcc ttcggtccaa ttggatagcg gaaatacacg
      181 gacggtttct ccggcattga tccgcgtgtt gtaaagattc caaagctccg gacgttgcga
      241 cttcggatcc caacgatgac cgacagctcg aaatgaaaaa atgcgctcct tggcgcgcga
      301 tttttcttcg tcgcgccggg caccgccgaa gacgaagtcg aagccgtgga gatcaagggc
      361 gtgcttgagc gcctcggtct tcatcacatc agtgtggtat gccgagccgt gcgtgacagg
      421 cccgatgcct tcggcagcgc cgcgcgggtt ggtaaacgta atgagttgca acccgagttg
      481 ttgtgccgta cggtcgcgaa atgcaatcat ctcgcggaac ttccagcccg tgtcgatatg
      541 catcaacgga aatgggatgg gcgcgggata aaacgctttc tgcgccaggc gcagcattac
      601 cgaagagtct ttaccgatcg agtaaagcat gacgggcttt cttgcggtgg ccgccacctc
      661 gcggatgatt tcaatggact cggattcgag ctctgagcaa acgtcgtagt tcataggaac
      721 agttgactga gaaaatcaga tccttatagc gtaccttgct tagagacgtc cagcggcgat
      781 ccggcggccc tattccgggc gcggacgatg cggctccggt gattaattcg aacatcgcat
      841 ttgacttgcg gacgagaagg aaattggcat acgtatctag agcaggtgtg caatgggcga
      901 caatttgcgc tgcataggag gtaagcgatg ctaaagacgc atggggtgct acatttcagt
      961 ttgccggtga ccgatctaga tcgcagtcgt aatttttacg agcaattgct cggcatgaaa
     1021 gtcgtcgaac aatcgccgcg gatggtcttc ctacagaccg gtgacgacta cttaatcttg
     1081 gccaagggaa acgagccgct gaaatacgat tcatcgaaaa gcacgccggt gcaccatgct
     1141 ttcaaagtga agcctgagga tttcgaacct tcgatcgagt ttctgcgcag aaacggtgtt
     1201 gaagtgttca acatcgaaga ccgaaaccaa ggagtcttct ggggaccgca ggcctatttt
     1261 ctagatccgg acggaaacaa gctggaaatc tatgccgggc cgggggcgaa gaacagttga
     1321 ggattggcct atcgaagtac aaagatttca ggtggcggaa aatagaatct cggaaaaatt
     1381 tcttcctacg gtcgaaatca cgttgacata ggaaccagca gcgtactcaa ccaaagcacg
     1441 ctgctcatga tcgtttagtg taggccggtg ttgatcagtt ctacttgcct cgggttgcgg
     1501 tccacttcgc accactggcg gtgccgtcga ttgaatttcc gttcacattc cccgtgtact
     1561 gagtgctgcc cgccgtaaac gtgatctggc tgccgtgcag cttaccgtta atcggcgtga
     1621 ccccagagcc attcttcagt gttccagtaa tattctgaaa catttgtttg atcgtcaaat
     1681 cgccttgcgc cgttttccac gtgccgtctg ctttcgccgg cacgatccat agatgagcgg
     1741 tgcaccagtt ggtgcaattc tcgaccgtgg cgtcttcatc gggcttccaa tcttccatat
     1801 caaaagtgtt cgagacgatc cgagtgccag gtttcaaatt caagagtttc gggcgcaatt
     1861 tcatattgat gctcggcagc aagaacatgg tgatgacctg agcctgcgag aagtcggtct
     1921 cgaacaggtc cgctttcatg aagctcgcct tgtcgctcac gccttctttg gcggcattgc
     1981 gctttgagag ttccaccatt tccggattgt attcgatgcc gagggctttc gagccgcgct
     2041 tcgctgcagt gatcaccgtg cgtccgtccc ccgaaccgag atcgattacg taatccttcg
     2101 gtgtcacttt agccatatcg agcattttgt cgacgagcgc ctggggtgtg ggtacccaga
     2161 caacgtcttt acccgcttgg ccgacctccg gttgaaactc cttcgccggc tgcgcttgcg
     2221 ccagagcggc cgcgagcaga atcgagagcg cagcccaaaa attataagca aaataatgtc
     2281 ggttcataga gtcctcccaa gatgaagtca aaaactgttt ataacaagcc catgaggcgg
     2341 acgggagagg tccggcaaaa aaaccatatg ttcgct
//
LOCUS       DASULZ010000688         2098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1195555, whole genome shotgun sequence.
ACCESSION   DASULZ010000688 DASULZ010000000
VERSION     DASULZ010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195555"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VFY96_14260"
     CDS             <1..720
                     /locus_tag="VFY96_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX5607684.1"
                     /translation="RLQDIFLDTIYISAHKRTITPKSIAQKAYIEAIRRYDIVFGIGP
                     AGTGKTYLAMAMAVAELMKNNYVRIILTRPAVEAGEKLGFLPGDLAEKVNPYLRPLYD
                     ALHDMVDFDRARKLVERGTIEVAPLAFMRGRTLNDSFVILDEAQNTTPEQMKMFLTRL
                     GYGSKAVITGDATQIDLPAGKPSGLKEASRILKGVHGIRFINFTEKDVARHHLVQEII
                     TAYERAENQSGTHEPQETSSS"
     gene            complement(739..1707)
                     /locus_tag="VFY96_14265"
     CDS             complement(739..1707)
                     /locus_tag="VFY96_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5607685.1"
                     /translation="MPSDEKSTINLFRQASPAVVNITAIGVERDLFTLNLYQIPQGTG
                     SGFVWNTNGDIITNFHVIQNADAAQVTLADQSNWKARVVGVAPDKDIAVLRIDAPANR
                     LHPIPIGTSKDLQVGQSVFAIGNPFGLDQTLTTGVISALNREIESVTRRPIQGVIQSD
                     AAINPGNSGGPLLDSAGRLIGVNTAIYSPSGASAGIGFAIPVDIVNRIVPELIRSGKV
                     TRPGLGIQIADEQIAERLGVTGVLVVDVTRGGAAAKAGIQPTRRDSEGRLRLGDVITA
                     IDGKKIESSNDLYLILEKYKVGDAVSVTMLRNGKSVQARVTLEEVR"
BASE COUNT          475 a          590 c          590 g          443 t
ORIGIN      
        1 cgtttgcagg acatctttct cgataccatt tacatttccg cccacaaacg cacaatcacg
       61 cccaaaagca tcgcgcagaa agcctacatc gaagccatcc gccgctacga cattgtcttc
      121 ggcattggtc ccgccggcac cggcaaaacc tatctcgcca tggcgatggc ggtggccgag
      181 ctcatgaaaa ataattatgt gcggattatt ctcacgcgcc cagccgtaga agcgggtgag
      241 aagctcgggt tcttgcctgg cgatctcgcc gaaaaggtca atccttacct tcgaccgctg
      301 tacgatgctt tgcacgacat ggtggatttc gatcgcgcgc gcaagctggt tgaacgcggc
      361 acgatcgagg tcgcaccgct cgcgttcatg cgggggagga cgctcaatga ttcgttcgtg
      421 attctggacg aggcgcaaaa caccacgccc gagcagatga agatgtttct cacccgattg
      481 ggctacgggt caaaagcggt gatcacaggc gatgcgacgc agattgattt accggcagga
      541 aaaccctcgg gcctgaagga agcgtcacgg attttgaaag gcgtgcacgg cattcgcttc
      601 atcaatttca cggaaaaaga tgtggcccgc catcatctgg ttcaggagat tatcacggcc
      661 tacgagcgcg ccgagaacca gtcaggcacg catgaacctc aggagacgag ttcgtcttag
      721 aacagtcatc gtttgcattt aacgaacctc ttcgagggtc acccgcgcct ggaccgactt
      781 gccgttgcgc aacatcgtca cagacactgc atcgcccacc ttgtatttct cgagtatcag
      841 ataaagatca ttggacgatt cgattttttt gccatcgatc gccgtaatga catcgcccaa
      901 gcggaggcgt ccctcgctat cccgtcgcgt cggctgtatg ccggccttcg ctgcggcgcc
      961 gccgcgcgtg acatccacga ccagcacgcc agtcacgccg agtctttcag cgatttgctc
     1021 gtccgcaatc tgaatcccta gtcctggcct ggtcaccttg ccgctacgaa tgagttccgg
     1081 cacgatgcga ttgacgatgt ccaccggaat ggcaaagccg attccggccg aggcacccga
     1141 agggctgtag atcgcggtat tgacaccgat caaacggccg gcgctgtcga gcagtggacc
     1201 gcccgaatta ccgggattga tggccgcatc agattggatc acaccttgaa tcgggcgacg
     1261 cgtcacggac tcgatctcgc gattgagcgc cgaaataacc ccggtggtta aggtttgatc
     1321 gagcccgaaa ggattgccga tcgcaaaaac actctggccg acctgtaaat ctttggatgt
     1381 tccgatcgga atgggatgga gccgattagc cggcgcatcg atgcgcagca cggcgatgtc
     1441 cttatcgggt gccactccca caacacgcgc cttccagttc gattggtcgg cgagcgtcac
     1501 ctgtgcggcg tcggcatttt gaatcacgtg aaaattagtg atgatatcgc cgttggtatt
     1561 ccagacgaat cctgagcccg tcccctgagg aatttgatag agattgaggg tgaaaagatc
     1621 gcgctcgaca ccgatcgccg tgatgttcac aactgccggc gatgcttgcc ggaagaggtt
     1681 tatggtcgat ttttcatcac tcggcaattc gcctcgcggg ctaaccgctc gcggggcggc
     1741 ccgcgggtcg aagagcgaag cactgcgaac ccaggagcct ttgaagaaaa acaagcccgc
     1801 cgcactcact gcgccggcta agactagata aaaccaaggg gagcgttgcc tggagctgcg
     1861 atggccgagt aacattgaag tttattcttt cgacgcatga agtatcggtc tagccggtgc
     1921 aaattctcat agcattttgc agctaaaaat tccaaagcga gtccgttcga gggaggccga
     1981 gtcgccctgc gagttgaata ggtttggagg agaggaactt cgggcgatgc atcgcgcgaa
     2041 cagtttctcg cgcgcggtgc atcgcccgag tggattggga cagaacagaa gttactct
//
LOCUS       DASULZ010000689         2673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1499816, whole genome shotgun sequence.
ACCESSION   DASULZ010000689 DASULZ010000000
VERSION     DASULZ010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499816"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VFY96_14270"
     CDS             complement(<1..426)
                     /locus_tag="VFY96_14270"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEX5607686.1"
                     /translation="MANNKRGKQALPAVTHKAPNWPVFALALIGMALSGYLTFTAWQG
                     KLVAFCTEGAGCDVVLNSRWSTLFGVPTSFWGFLTYTLLAAVAWNKNAADRWRWGWTI
                     SLFGLLFSFYLTSVSLFELKATCPYCLTSLAIMAAIFIVT"
     gene            complement(434..1084)
                     /locus_tag="VFY96_14275"
     CDS             complement(434..1084)
                     /locus_tag="VFY96_14275"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX5607687.1"
                     /translation="MFDNTKTRFLLLGVAVVGLGVILGTALWLKLAPLSRLAVEQNDS
                     SNGVKQYGSVPEFTLTERDGSVVSLQQLRGKIWVADFIYTSCTDTCPLQSAMMAKLQQ
                     QYATNPDFQLVSFTVDPQRDTPQALTSYATKFQADGKRWYFLTGQRERILRLVEEGFH
                     LAVATFSNDTDSAGVIGHSPRFVLIDKDAQIRGYYDIRESEAFVRLKDDIDSLVKG"
     gene            complement(1226..1774)
                     /locus_tag="VFY96_14280"
     CDS             complement(1226..1774)
                     /locus_tag="VFY96_14280"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HEX5607688.1"
                     /translation="MALLLLCSQLGLNAVPALGVESALGILEIQLTDHREAIGDFSQV
                     KLLIEKILLSPRSGLQFWRTGWQEIAPSSDSIDLTQLTGKKTARIFRGEIPPGAFDAF
                     NIKIKTISAVLKKTRRSASIKNTVGPVKLAFEVPAQGETLLVIDLVVTDFSDHPPQGY
                     ELAIKGYELYTNGKLVEKIPPE"
     gene            complement(1789..2424)
                     /locus_tag="VFY96_14285"
     CDS             complement(1789..2424)
                     /locus_tag="VFY96_14285"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX5607689.1"
                     /translation="MSSKSIIRRSLPLALLALGVFSSAAWSHIPIPPKSPDKIGRREV
                     KISVPDFNLKDQDGKTFRFANLRGQLVVITFIFTTCPDVCPLLTAHFAAIQRALEEQK
                     IKDYQLLSITTDPERDTAPVLKDYARRFKADLRFWSFLTGSRLELAKVWHAFGVNVVK
                     NQSGQVQHTTFTTLVDRHGNRRVDYYGDRWRDTEVLRDIQWLRGNSVSQNL"
     gene            complement(2402..>2673)
                     /locus_tag="VFY96_14290"
     CDS             complement(2402..>2673)
                     /locus_tag="VFY96_14290"
                     /inference="COORDINATES: protein motif:HMM:NF016337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX5607690.1"
                     /translation="ETVARPSDVFAIGFRVKNRTDREIVTRIVHRIEPKELAQYLELV
                     QCALLLPVRLRPGEEEIYNSTYVVRADLPEGTKRLDVIYEFKVDN"
BASE COUNT          634 a          698 c          745 g          596 t
ORIGIN      
        1 ggtgacgata aaaatcgcgg ccataatcgc gagggacgtc aagcagtaag ggcaggtggc
       61 tttgagctcg aacaacgaaa ccgaggttag atagaaactg aaaagcaaac cgaataatga
      121 aatcgtccaa ccccagcgcc accgatccgc agcattttta ttccaagcga cggcagcgag
      181 cagtgtatag gtgagaaaac cccagaacga ggtcggcaca ccgaaaagcg tcgaccagcg
      241 gctgttaagc acaacatcac agccagcgcc ctcggtgcag aatgcaacga gcttgccttg
      301 ccatgcggtg aaggtcagat agccggaaag tgccatgccg atcagggcga gagcaaatac
      361 tggccagttt ggcgctttgt gggtcaccgc cggtagcgct tgtttgcccc tcttgttgtt
      421 agccatcttg ctcttaacct ttcactaagg agtcgatgtc gtctttgagc cggacgaacg
      481 cttccgattc tcggatgtcg taataaccac gaatctgggc atctttatca atcagaacaa
      541 agcgcgggct gtgcccaatg acacccgcgg aatcggtgtc gttggagaaa gtggcaaccg
      601 cgagatggaa cccctcttcc accagacgta gaatccgctc tctctgcccg gtgagaaaat
      661 accaacgctt gccgtccgcc tgaaatttcg tggcgtaact cgtgagcgct tgcggggtgt
      721 cacgctgcgg gtcgacggtg aacgaaacca actgaaagtc tgggttcgtc gcatattgct
      781 gctgcagctt tgccatcatg gcgctttgca gcggacatgt atcggtacag ctggtataaa
      841 tgaaatccgc gacccaaatt ttgccccgta gctgttgcaa gctgacgaca ctgccgtcac
      901 gttccgtcaa agtaaactca ggcaccgaac catactgctt cacgccattc gaggaatcgt
      961 tttgctcgac ggccagacgc gaaagcggcg caagtttcag ccaaagcgcc gtgccaagga
     1021 tcactcccaa acccacgact gcaacgccga gcagcaggaa gcgagttttt gtattatcga
     1081 acattgcgat gaccaaacag cgacgattct aacgagaaaa ataagctttg tgtagagata
     1141 tataccgtcc ggcctaatac ggctgggctg cgatgaatcg gccgtgtgcg cccattaggc
     1201 ccatatcagt ttgcctgctg cgcgattatt ccggtgggat tttttcaacc agcttgccat
     1261 tggtataaag ctcataacct ttgatcgcta attcgtatcc ctgcggtgga tggtcggaga
     1321 agtctgtgac gacgaggtcg atcacgagaa gagtctcgcc ttgcgcaggt acttcaaatg
     1381 cgagcttaac gggtccgacc gtattcttta tcgacgcgct gcggcgggtt tttttgagga
     1441 cggcgctgat ggttttgatc ttgatattga acgcgtcgaa agcgccggga ggaatttcgc
     1501 cgcgaaaaat acgcgcggtt tttttcccag tcaattgagt caaatcgatc gaatcggaag
     1561 agggagcgat ttcttgccat ccggtgcgcc agaattggag cccggatctc gggctgagaa
     1621 gaattttttc gatcagaagt ttgacctggc taaaatcgcc gatcgcttct cgatgatccg
     1681 tgagttgaat ctccaggatg ccgagcgcgc tttcgacgcc gagcgcgggc acggcgttca
     1741 accctagctg ggaacaaagt aacaagagcg ccaaaaagtt cgaccgtctc atagattttg
     1801 tgagacgcta ttcccgcgca gccactggat gtcgcgcaaa acctctgtat cgcgccacct
     1861 atcgccataa taatcaactc gacgattccc gtgacgatcc acgagagtcg tgaatgtggt
     1921 gtgctggact tgcccggatt gattcttgac aacgttgacg ccgaaggcgt gccagacttt
     1981 ggcgagctcc agccggctgc cggtgagaaa agaccagaat ctcaaatccg ctttgaagcg
     2041 gcgggcgtaa tctttcaaga ccggcgcggt gtcgcgctcg gggtcggtgg tgatgcttag
     2101 cagctggtag tctttgatct tttgttcttc gagggctcgc tggatcgctg cgaaatgcgc
     2161 cgtcaatagg ggacacacat ccgggcaggt agtaaagatg aaggtgataa caaccagctg
     2221 tccccggaga ttcgcgaaac gaaaggtctt gccgtcttgg tccttaaggt tgaaatccgg
     2281 taccgatatt ttgacttcgc gccggccgat cttgtccggg gacttcggcg gaatgggaat
     2341 gtgcgaccaa gccgccgaag agaagactcc cagcgcgagg agcgccagag gaaggctccg
     2401 tctaattatc gactttgaac tcatagataa catcgaggcg cttggtccct tcggggagat
     2461 cggcgcggac aacgtaagtg ctgttataga tttcctcctc tcccggacgg agccgcacgg
     2521 gaagcagcag agcgcattgc acaagttcca aatattgcgc cagttctttg ggctcgatcc
     2581 gatgcacgat tcgggtcacg atttcgcgat ccgtgcggtt tttaaccctg aagccgatcg
     2641 cgaaaacatc actcggacgg gcgaccgttt ctt
//
LOCUS       DASULZ010000690         6173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_80066, whole genome shotgun sequence.
ACCESSION   DASULZ010000690 DASULZ010000000
VERSION     DASULZ010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80066"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VFY96_14295"
     CDS             complement(<1..222)
                     /locus_tag="VFY96_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5607691.1"
                     /translation="MALSREENETLTRVAPGTAAGEMLRRYWHPIGFAAELRDKPLRR
                     RILGEDVVLFRDEGGRFGVLALRCMHRGTS"
     gene            698..4165
                     /locus_tag="VFY96_14300"
     CDS             698..4165
                     /locus_tag="VFY96_14300"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HEX5607692.1"
                     /translation="MTKILLVAGVYFCAGKLGLSWAHIHISVSAVWPPSGLALAALLL
                     WGCRLWPGIFVGAFVLNATTQGTVVTALGIATGNTLEALFAAWAVNTFANGAKAFERA
                     RNTFRFVVLAPLLSPVVSATLGTLSLTVSGFARPEQFGAIWLTWWLGDAVGELIFAPF
                     IIIWLTQPLPRAKPKRVIEASSLLIFLSAVGHFVFMRADSSSPEYLVMLPLLWAAFRF
                     GQRGAVTSALIMSVIALAGTLYGVGPYAEADPNESLLHLQSFMSTLEIAALVLASVLC
                     EAKRAEQRLEVQESISRVLAESPAVKEAAPLIIEVLCRRAGWDIGAVWIMDRSASELQ
                     YVDVWHVPGLEAPEFERVTIHGRIKRGTGLPGRVLSSGKAIWISDVTMDAEFRRAPLA
                     EKEGLHGAFAFPIKLGQETLGVVECFSREVREIDDHFLEMVSDIGAQLGQFIHRKRAE
                     DDVRAQRLQLRAITDVTPLLLTQCTRDFTYEFVNRAYAALLGTTPEEIIGKSIWDVIG
                     AEGLETIRPYIESTLSGRTVEYEAEIRFARIGPRFLRVVDTPVTDDQGQVRGWIASMS
                     DITERKRTEDEIAALNHQLAQREEHLRAVIETAVDGIITIDEDETITTVNPATERIFG
                     YFAAEMVGQNLRKLIPTSSASEADSLVAKDLSGRKKNFLGMAGEVEAKRKDGTPFPLD
                     LAVSETRIGDRRIFIAILRDISDRKRASDLLRQARDELVKANQDLEKRVRERTADLEQ
                     ANAALLRTIAGQKRLEDQLRQAQKMESIGTLAGGIAHDFNNILNIIRGYATLIGQQPL
                     TAEQLEESLRVIDKEIDRGASVVRQLLTIARKSDTHLAPTNISDIVLTLNDLIKTFPK
                     NISVSLNLDTRLMPVLVDRNQMSQVLLNICLNARDAMPAGGKLTIQTEKIDALEMRER
                     HDVDAASGAYVCVTIGDTGTGITEEVRARMFEPFYTTKGISEGTGLGLAIVYGIVKDH
                     NGFIEAESEPGKGTTFRVYLPVLESAEMPVTKEHEKQRAVSAKLDHRRGTILVVEDEE
                     PLVRLLNKVLPREGYQVLAATNGEQGIDLYQVHKSEIDIVLLDLGLPKMTGMDVIPKL
                     REQNPDVNIVIATGYLEPEVKAELLRAGVRDCINKPYLLNEVLEKLDAVLESPRNLAS
                     VREPRDCPS"
     gene            complement(4279..5268)
                     /locus_tag="VFY96_14305"
     CDS             complement(4279..5268)
                     /locus_tag="VFY96_14305"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607693.1"
                     /translation="MKKPATLVALVLFCFTAVSPNAAQERKLEKIRVGGGSVGAPQMT
                     MWFAKEANLYEKHGLAVEAISIPGSSMALQAMLSGEVPIIQLGGTASMLANFAGADTV
                     IVATVIKKFVFSIFSRPEIARFADLKGKLFGATRFGTLSEFASRFALERNGLNPDRDV
                     AMVQTGGQPETVVALLTGKVQGAALSVPAILRAKKANMRELLDMAKLEATIHQNGVVT
                     TRKYLKTNEDTVRRFLRAYIEGAALAKKDKAFATRVMAKYLGTNDRELLDDAYERVTL
                     HLEVPPYPTLEGVGVLLKTLEKTQPKARTAKPEDFIDSRLVRELDETGFVNRF"
     gene            complement(5283..>6173)
                     /locus_tag="VFY96_14310"
     CDS             complement(5283..>6173)
                     /locus_tag="VFY96_14310"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5607694.1"
                     /translation="SAPYIKDADVVVVVESDVPWYPVQGKPPENCTVIQIARDPNYGS
                     IPIRNFPKDISIGGDPKLALDLLARELKSQGRNEKALADRAESIRLRHRQNRQRAKEK
                     AEKASRERPIDTAWLSHCVDQVRDANTLIVNDHGVSQEQLSLSEPGSYFGGSPAGGLG
                     WGIGGGLGMKLAHPEKTVIVSCGDGTYMFNNPTACHFVSQSYHLPLLTIVCNNAVWHS
                     TKAATQQVMPDGWAVSTDNYPLCDLTPSPRYEKIVAAHDGYGEMVDDPQQLPAALERA
                     LKIVKEEKRQAVLNVICGTH"
BASE COUNT         1385 a         1724 c         1706 g         1358 t
ORIGIN      
        1 tgaggtgccg cgatgcatgc agcgtaacgc cagtactccg aatcgccccc cttcgtcgcg
       61 gaacagcacc acgtcttcgc ctaaaattct tcggcgtaac ggcttgtcgc gcagctcggc
      121 ggcgaagccg atcgggtgcc aatagcgccg caacatctcg cccgctgccg ttccgggtgc
      181 gacccgagtc aacgtttcat tttcttccct gcttagcgcc atacggatcc tcttcaacgc
      241 agtgaagagc tgagctcacg acagtcgttc gggtcagaaa tatctgagat gttcttgacg
      301 tgtcaaggcg aactatacag cgattcaaca gtggccgact gcccgttcta tctcaacttg
      361 ttattgcgag cagcgagaaa tccttctcga gaacgttttg gtgcgaagag tccgaaccca
      421 atcaacttct agaaaaattt ctcgaatgac acataaaacc cggcaagtaa ttaaccgaca
      481 aaaattcggg ccctctgttc agcggcgctg aagagatccc agctcctatg attggacggg
      541 agttctaatc gttcgctcgt ctcatcactg cggataaaaa cggtctttaa acttattgac
      601 aaaatcgaaa atcggcgact atcgtcggat gctcccaatg agacacccaa taataactcg
      661 agtcacaagg actctgggcc aaacggccgt tggggacatg acaaagatcc tgcttgtcgc
      721 tggagtctat ttctgcgccg gcaagctggg gctttcttgg gctcacatcc acatcagtgt
      781 ttccgccgtt tggccgccga gcgggctcgc cttggcggcg ctcctactct ggggctgccg
      841 gttatggccc ggaatctttg tcggcgcgtt tgttttgaac gccacgacgc aaggaacggt
      901 cgtgacggca ctcggcatag cgactggaaa tactctcgaa gcactgtttg cggcatgggc
      961 agtcaatact ttcgctaacg gcgccaaagc cttcgagcgg gcgcgaaata ctttccgttt
     1021 cgtcgtgctc gcaccattgc tgagcccggt cgtaagcgcc accctaggga ctctaagttt
     1081 aaccgtgagc ggcttcgccc ggccggagca gttcggcgcg atttggctga cctggtggtt
     1141 aggcgatgcg gtgggcgaac tcattttcgc cccgttcatc attatctggc tgacccaacc
     1201 tttacctcgg gccaaaccga agcgcgtgat cgaagcctcg agtctgctga ttttcctcag
     1261 cgccgtcggt cattttgttt ttatgagagc agattcctca agcccggaat atctcgtcat
     1321 gctgccgctt ctatgggcgg ctttccggtt cggacaacgc ggcgccgtga cttccgcatt
     1381 gatcatgtcg gtaatcgcac tggctggaac cctatacggc gttggcccct acgccgaagc
     1441 ggatcctaac gaatccttac tgcatttgca gagcttcatg agcactctgg aaattgcggc
     1501 attggtactc gcatccgtcc tctgtgaagc aaaacgggcg gagcaacgtc tcgaagtgca
     1561 agaatcgatc agccgtgttc tcgctgaatc gccggcggtt aaagaggcgg ctccgttgat
     1621 tattgaagtg ctctgccggc gagctggttg ggatattggc gccgtgtgga tcatggatcg
     1681 ttccgccagc gaactccaat acgtcgatgt ttggcacgta cccggcttgg aagcacccga
     1741 atttgagagg gtaaccatcc acggtcggat caagcgcggc accggtttac ctggccgagt
     1801 tttgagcagc ggcaaggcga tctggatcag cgatgtcacc atggacgcgg agtttcggcg
     1861 cgcaccgctg gccgagaaag aaggactgca cggggctttt gcattcccaa tcaaattggg
     1921 tcaggaaacc ttgggcgtcg tggaatgctt cagtcgtgag gtgcgcgaga tcgacgacca
     1981 tttcttggaa atggtgagtg acatcggagc gcaacttggg cagttcatcc accgcaaacg
     2041 tgccgaagat gacgtgcgcg cccagcgact gcaacttcga gccattacgg acgtgacacc
     2101 gcttctattg acgcaatgca ctcgagactt cacctatgag tttgtcaacc gcgcttatgc
     2161 cgccctgctg ggcaccactc cggaagaaat catcggcaaa tcaatttggg acgtgatcgg
     2221 cgccgaaggt ttggaaacca tacggccgta tattgaaagt actttgagcg gacgcacggt
     2281 ggagtacgag gcagagattc gatttgcgcg gatcggaccc cgttttttgc gcgtggttga
     2341 cacgcccgta accgacgatc aaggacaggt ccgcggctgg atcgcctcca tgagcgacat
     2401 caccgaacgc aaacgcacgg aagatgagat tgccgcgctc aaccaccaac tcgcgcaacg
     2461 tgaagagcat cttcgggcgg tgatagaaac agcggtcgac ggcatcatta ccatcgatga
     2521 agatgaaaca attaccactg tgaacccagc gacggagcgc atttttggct attttgcagc
     2581 ggagatggtc ggtcaaaacc tccgcaagct gatcccgact tcgtccgcgt ccgaagcaga
     2641 ttcgctcgtc gctaaagatc tgagcggacg caaaaaaaat ttcctcggca tggccggcga
     2701 agtcgaagcc aagcgcaaag acggcacgcc cttccccctc gacttggcgg tgagcgagac
     2761 tcggatcggc gaccggcgta tttttatcgc aattctgcgt gacatctcgg accgcaagcg
     2821 agccagcgac ctcctcagac aggcacgaga cgaactggta aaagccaacc aggatttgga
     2881 gaagcgcgtg cgcgagcgga cagccgatct cgagcaagcc aacgcggctt tgctccgaac
     2941 gattgcagga caaaagcgac ttgaagacca gttgcgccag gcacaaaaaa tggaaagcat
     3001 tggcacactc gctgggggca tcgctcacga tttcaacaat attctgaaca ttattcgcgg
     3061 ttacgcgacc ctgatcggtc aacaaccctt gaccgcggag cagcttgaag aaagcttgag
     3121 agtaatcgat aaggaaattg accggggcgc ttcggttgtg cgccaactct tgaccattgc
     3181 gcgcaagtcc gacacgcatt tggcgcccac aaatatcagc gacatcgttc tcacccttaa
     3241 cgatctcatc aaaacttttc ctaaaaacat cagcgtgtca ctgaacctcg acacgaggct
     3301 gatgcccgtc ttggtggatc gcaatcaaat gagtcaggtg ctgctcaata tttgcctcaa
     3361 tgcccgtgac gcgatgcctg cgggcggcaa gctcacgatt cagacggaaa aaattgacgc
     3421 gctggaaatg cgcgagcgcc acgacgtcga cgcggcatcg ggagcctacg tctgtgttac
     3481 gatcggcgat acgggaaccg ggatcacaga agaagtgcgt gcccgcatgt tcgagccgtt
     3541 ctacacaact aagggaataa gcgaaggaac cggtctcggc ctggccatcg tctacggcat
     3601 tgtcaaagac cacaacggct ttatcgaggc cgaaagcgag cccggaaaag ggacgacgtt
     3661 ccgagtttac ttaccagttc tggaatcggc ggaaatgccc gtgacaaagg aacatgagaa
     3721 gcaacgcgct gtctctgcga agctcgacca tcgacgcggc accatcctcg tcgtggaaga
     3781 cgaagaaccc ctggtccggc tgctgaacaa agttttgcct cgcgagggat atcaggtttt
     3841 ggccgctaca aatggcgagc aagggatcga tctctatcaa gtccataagt cggaaattga
     3901 cattgtgctt ttggatctcg ggttaccgaa gatgaccgga atggatgtaa tcccgaaatt
     3961 gagggagcaa aatcccgacg ttaacatcgt gatcgccacc gggtatctcg agcctgaagt
     4021 gaaggccgaa cttctccgcg ccggcgtcag agactgtatc aacaagccct atttgctcaa
     4081 tgaagttttg gaaaaattgg acgccgttct tgagtctccg cggaatttag catcagttcg
     4141 cgaaccgcgc gattgtccga gttaacgcgc actgctttga tcgaattccg cgctgtgtcg
     4201 tgccgagcgg cagcgaagca actcgagccg ttgattccac cacaaagggg aaaaacaacc
     4261 ttcctcgtgc aacctaattc agaaccggtt cacgaaaccg gtttcatcga gctcacggac
     4321 gagccgtgag tcgataaaat cctccggctt cgccgtccgc gccttgggtt gtgttttttc
     4381 taacgtcttt agcaaaacac ccactccctc gagcgtcgga taaggcggca cctcgagatg
     4441 aagcgtcacc cgttcgtagg cgtcgtcgag cagctcacgg tcgttggtgc cgagatactt
     4501 cgccatcact cgtgtggcga acgctttgtc ttttttggcg agcgcggcgc cttcgatgta
     4561 ggctctcaaa aaacgccgca cggtgtcttc gttggtcttg aggtattttc gcgtggtcac
     4621 gacgccgttc tgatgaatgg tcgcctccag tttagccata tcgagcagtt cgcgcatgtt
     4681 ggctttcttg gctcgtaaga ttgctggaac ggacagtgcg gcgccttgaa cttttccggt
     4741 aaggagggcg accacggtct cgggttgtcc accggtttgt accatcgcga cgtcccggtc
     4801 aggattgaga ccgtttcttt ccaacgcaaa ccgcgatgcg aactcggaga gcgtgccgaa
     4861 gcgcgtcgcg ccgaagagtt tccctttaag atcggcgaac cgagcaatct ccggtcggct
     4921 aaagatcgag aagacgaact tcttaataac ggtcgctacg atcaccgtat cggcgccagc
     4981 gaaatttgcc agcatggaag ccgtgccgcc tagctgaatg atcggcacct cgcccgaaag
     5041 catcgcctgc aatgccatgg aactgccggg aatcgatatc gcttcgacag cgaggccatg
     5101 cttctcataa agattcgcct ctttggcaaa ccacatcgtc atctgcggtg ctcccaccga
     5161 gccaccgccg acgcgaatct tttcgagctt tctttcctgt gctgcgttgg gcgagaccgc
     5221 cgtgaaacaa aacaagacaa gtgccacgag agtcgcaggc ttcttcataa cgatttaatg
     5281 cttcagtgcg tgccgcagat gacattcagc acggcttggc gcttctcttc tttaacgatc
     5341 ttgagcgcgc gctccaaagc agcgggcaat tgctgcggat cgtcgaccat ctcgccgtac
     5401 ccgtcgtgag cagcgacgat tttttcatag cgcggcgatg gcgtgagatc gcacagtgga
     5461 tagttgtccg tgctcaccgc ccagccatcg ggcatgacct gctgagtcgc cgctttcgtg
     5521 gaatgccaga cggcgttgtt acagacgatt gtgagcagag gcagatggta tgactgcgac
     5581 acgaaatggc acgcggttgg gttgttgaac atataggtgc cgtcgccgca gctgacgatc
     5641 acggtttttt ccgggtgggc tagcttcatt cccaacccgc cgccgattcc ccaacccaag
     5701 ccgccggccg gagaaccgcc gaagtagctg cccggctcgc ttagactgag ctgttcctgc
     5761 gagaccccat gatcattgac aatcagcgta ttcgcgtccc tgacttgatc gacgcaatgg
     5821 gataaccatg cggtgtcaat cggccgctcc cggcttgcct tttccgcttt ctctttcgcg
     5881 cgttgacgat tttgccggtg gcgtaggcga atgctttccg ctcgatccgc caaggctttc
     5941 tcgtttctac cctgtgattt caactcgcgg gcaagcaagt ccaaagcgag tttgggatca
     6001 ccgccgatgg aaatatcctt gggaaaattt cgaatcggga ttgagccgta gttaggatcg
     6061 cgagcgatct gaatgaccgt acagttttcg ggcggtttgc cttggaccgg ataccaaggc
     6121 acatcggact cgaccaccac gacaacgtcg gcatctttga tatagggggc gga
//
LOCUS       DASULZ010000691         2730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_688575, whole genome shotgun sequence.
ACCESSION   DASULZ010000691 DASULZ010000000
VERSION     DASULZ010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688575"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(300..764)
                     /locus_tag="VFY96_14315"
     CDS             complement(300..764)
                     /locus_tag="VFY96_14315"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX5607695.1"
                     /translation="MFGDLIRWNPAEELTNWHRDIDELFGRFFQRPEGSMQSWMPRME
                     TCRKDNNYVVRIDLPGVNPNDVHVETEGNLLTISGERKGEEKGSEYRETFYGKFERQI
                     TLPQGVEADKVTARYEHGVLEIQVPLPAQLAARKVPIQIEEKDKRQLESKAA"
     gene            complement(953..1951)
                     /gene="tsaD"
                     /locus_tag="VFY96_14320"
     CDS             complement(953..1951)
                     /gene="tsaD"
                     /locus_tag="VFY96_14320"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX5607696.1"
                     /translation="MLILGVETSCDDTAAAVLQDGTKILSNVVSSQDQVHGAYGGVVP
                     ELASRQHIRNILPIIDAALANASVKLADLNGIAVTYGPGLVGSLLVGLSLVKGISFRS
                     GRPYVGVNHLEAHLLAIQLEHQVPFPYIALLASGGHTLLYRVNNVGEYLHLGGTRDDA
                     AGEAYDKVAKMMGLAYPGGRIIDNLAKEGNPRAIRFPRARLKKNSYDFSFSGIKTAVW
                     HYLNSGGREQCQAPKQDVAASFQEAVVDMLIDPTIKAAVAHGIGRIVLSGGVAANSRL
                     RAKMTERAKEDQLQVFYPAPKFCTDNGAMIALTGYHWLRRDRRDDYALNADADLTL"
     gene            complement(1962..>2730)
                     /locus_tag="VFY96_14325"
     CDS             complement(1962..>2730)
                     /locus_tag="VFY96_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5607697.1"
                     /translation="GFDRRPFFSALSIASLLTCAACEPPRPYRIPVPPTSNPVLRTPP
                     PSPVPSPPPPDESARKPLPQEPNIREQDLKDKPQTERVLSKETKEPAPLPSTGEISAA
                     PEAPPAPLPDDSSLLAKITPGVTPQRAASLRLTEEGKKLLDSGDPTKALARLEKTIVI
                     DSTNAYGYFYLAKAQYRLGRYRESLNFLDIAESRLGNEPFWLAEIHALRGENYRTLGQ
                     LQSAEASYQQAVRLNSGNRTAADGLLRLQSDKSFSGK"
BASE COUNT          602 a          726 c          745 g          657 t
ORIGIN      
        1 tcgaatcgtt cggcaattac cgagcaaccg agatctctgg ggagactagg tatttcgatg
       61 atttcctgtg ttgaaaccga agcatcgttt tgaataaaat ttcttcgtgt ccaagagata
      121 gcaaatgttt tacatgactt agaatatcgc gctctacgtg aaaactccgc gcgacttttc
      181 gaaaagtcac tcctgctcca gctgatggaa aaaatttggc caaaaaaaag gggcccgtta
      241 gggcccctac aaccggctgc gggagagcgg tttgaagcga ctcgacggaa ccgaatcgct
      301 tacgcggctt tcgattcgag ttgtcttttg tccttttctt cgatctgaat cggcaccttt
      361 cgtgcagcaa gctgcgccgg aagcggcact tggatctcaa ggactccgtg ctcgtagcga
      421 gccgtcactt tgtccgcctc tacaccctga ggaagcgtga tctggcgctc gaacttgccg
      481 tagaacgttt cccgatactc agaccctttc tcctccccct ttcgttcgcc agagatggtc
      541 aggagatttc cctcggtctc gacatgaacg tcgttgggat tgacacccgg cagatcgatt
      601 ctaaccacgt agttattgtc cttccggcac gtctccatcc ggggcatcca gctctgcatc
      661 gagccttcag ggcgctggaa gaaccggccg aacaattcat cgatgtcacg atgccaattt
      721 gtcaactctt cagcaggatt ccatctgatc aaatcaccga acataatccg tttcctcctt
      781 tcgtcttgat ggaaattcca atgttaattt aattctcgtt caatagctgt caaggtcgtc
      841 cctgtggttt agggacctgg ctgcacctag gggtgccgcg ctttcaagtt caaacaacgc
      901 gctcaggtct caaaattcga ttttaatggt gaaataggat tatgcgaaga aattaaagcg
      961 ttagatcagc atctgcattg agcgcgtaat catcgcgacg gtcacgcctg agccaatgat
     1021 agccggtcag ggcaatcatc gctccattat cggtgcagaa tttgggggcg ggatagaaga
     1081 cttgtaattg atcttccttg gcccgttcgg tcattttggc gcgcaatcgg ctgttcgccg
     1141 caaccccacc agaaagaacg attcggccaa ttccgtgcgc cacggcggct ttaatggttg
     1201 gatcgattag catgtcgaca accgcttcct gaaagcttgc cgcgacgtcc tgttttggcg
     1261 cttggcactg ctcccgacca ccgctattga gatagtgcca tacggcggtt ttgatcccgc
     1321 tgaagctaaa atcgtaggag ttttttttaa gcctagcgcg tggaaaccga atcgcccttg
     1381 ggttgccctc tttggcaaga ttatcgatga ttcgtccgcc gggataagca agccccatca
     1441 ttttcgcgac cttgtcgtac gcctcgccgg ccgcatcatc gcgagtcccg cccaaatgga
     1501 gatattcgcc gacgttgtta actcgataca ggagcgtatg acctcccgaa gccagcagtg
     1561 cgatgtaagg aaacggcacc tggtgctcca gctggatcgc gagtaagtgc gcttccagat
     1621 gattgacacc gacgtaaggt cttccggaac gaaaagaaat gcctttgacg agcgagagtc
     1681 cgacgagcaa agagccgact agaccgggac cgtaggtcac ggcgataccg tttaaatccg
     1741 ctaatttcac gctggcgttt gcaagcgcgg catcgataat cggcaagata tttctgatgt
     1801 gctggcgtga tgcgagctcc ggcacaaccc cgccgtacgc tccatgcact tgatcctgtg
     1861 aagagacaac gttggagaga attttcgtgc catcctgtaa gaccgctgcc gctgtgtcat
     1921 cgcaggaggt ttccactcca aggatcagca tgaggcaaaa cctatttccc ggagaatgac
     1981 ttgtcgcttt gtagccggag caatccgtcc gcagcggtac gattacccga gttcaggcgc
     2041 accgcttgtt gatagctcgc ttcggcgctt tgaagttggc cgagagttcg ataattttcg
     2101 cccctgagcg cgtggatctc agcgagccag aatggttcgt tgcctaaccg ggactcggca
     2161 atgtccagaa aattgagcga ctcccgataa cgtccgagcc gatactgcgc cttggccaaa
     2221 taaaagtagc cgtacgcgtt agtcgaatcg atcacgatgg ttttttccaa ccgagcgagc
     2281 gctttggtcg gatcgccgct atcgagaagc tttttgcctt cttcggtgag acgcaaagaa
     2341 gcggcgcgtt gcggcgtgac tccgggagta atcttcgcga gcaatgagct gtcatctggc
     2401 agaggcgcgg ggggcgcttc tggagcagcc gagatttcgc cggtactcgg taatggcgca
     2461 ggttcttttg tctctttgct caggacgcgc tcggtctgcg gcttgtcctt aaggtcctgc
     2521 tcgcgaatat ttggctcctg aggcagcggc tttcgcgcgc tttcgtcggg cggcggcgga
     2581 gaaggaacag gcgatggcgg tggagtccgt aaaacgggat tgctggttgg cggaaccggg
     2641 attcggtatg gccgcggcgg ctcgcatgcc gcgcacgtca atagcgaagc aatactgagg
     2701 gcggaaaaaa aagggcgacg atcaaaccca
//
LOCUS       DASULZ010000692         4120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_688581, whole genome shotgun sequence.
ACCESSION   DASULZ010000692 DASULZ010000000
VERSION     DASULZ010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688581"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..921
                     /gene="pgl"
                     /locus_tag="VFY96_14330"
     CDS             229..921
                     /gene="pgl"
                     /locus_tag="VFY96_14330"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX5607698.1"
                     /translation="MKIEIFPDADAVAQKGAEIIAAEARTAVKARGRFVLAVSGGHTP
                     WRMLRALANQDVPWKQVHVVQVDERVAPEGDPDRNLTHLYSSLLEYAFLTREQISAMP
                     VEAPDLETAAKQYVETLKKTAGSPPVLDLVHLGLGPDGHTASLVPGDAVLNVTDRDVA
                     ITGVYQGRRRMTLTYPIINRAHGILWLVTGDDKVSALARLREGDPTIPAGGVQRDSAL
                     VLADQAAAPTEK"
     gene            complement(1647..2048)
                     /locus_tag="VFY96_14335"
     CDS             complement(1647..2048)
                     /locus_tag="VFY96_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX5607699.1"
                     /translation="MDKALTAFAPYAYAILRIIAGLSFICHSGQKLFGWFGGDPVPIA
                     SLFGVAGVIELILGVLITIGLFTSYAAFIASGEMAVAYFIGHFPKSFWPLENAGEPAV
                     LFCFIFLYMATQGSGIWSADAARRERAIAIP"
     gene            complement(2174..>4120)
                     /gene="ftsH"
                     /locus_tag="VFY96_14340"
     CDS             complement(2174..>4120)
                     /gene="ftsH"
                     /locus_tag="VFY96_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX5607700.1"
                     /translation="FLIVLVANYLIVRFLIPGPEAPVTIPYTFFKEEVAKHNVKSIYS
                     QGESITGRFASPVTYPPAEQKTAASSSKPEKPSPSGLTRTVPKTSPNFTTTLPSFVGP
                     GLEGFLINNGVEISAKPIEEGGGLISTLLFGFGPALLFIGFYVWMFRRAQQGGGLGGG
                     LMGIGKSRARRYDQEKDTKVTFDDVAGIDEAENELIEIVDFLRDPKKYTRLGGTAPKG
                     VLLIGAPGTGKTLLAKAVAGEAGVPFFSMSAAEFVEMIVGVGAARVRDLFKQARENAP
                     AIVFIDELDAIGRARGQVAIGGSSEQEQTLNQILTEMDGFSSREGIIVLAATNQPDVL
                     DKALLRPGRFDRRVIVNLPDKNGREAILKVHTRTVPLAKDVSLEEIAAATPGFSGADL
                     KNLVNEAALLAARREQNEVRQKDFLDSLEKIVLGPERPLLLSPADKERIAYHEGGHAI
                     LGLVVPGADPVNRVTIVPRGQALGVTYQRPDSDRYNYPEAYLRARIVGMLGGRAAEEI
                     VYGTKTTGAESDIEQATGLARRMVTRWGMSERLGLVQLAPRDNPYLNGQADFGTSKPF
                     SDETAEIIDSEVLKIITESHDQAKQLLIEHRKQLDALAQALVARETLNEQEILDVTGL
                     PRAPALDTAMLPVANGGASARHLI"
BASE COUNT          924 a         1171 c         1126 g          899 t
ORIGIN      
        1 aaaaaccggc attccgattt atccttacga gccaaatagc tggggaccgt gtgaggtcga
       61 gcagagcgtt tcaccgcccg gaggctggca taatccggtg gggacaggct gaaagcgcgg
      121 agtgatagag tattggagga atcgaaggtg gagtaatgga gtagcagagt attgaaacca
      181 attatcgata aatccaacac tccagtactc aattctctag gagagcccat gaaaattgaa
      241 atattccccg atgccgatgc ggtggcgcaa aagggtgccg agatcattgc tgcggaggct
      301 cgcaccgccg tcaaggcgcg cggccgtttc gtgcttgccg ttagcggagg tcacacgccc
      361 tggcgaatgt tacgtgctct agccaaccaa gatgtcccgt ggaaacaggt ccatgtcgta
      421 caagtggacg aacgtgttgc acctgagggc gatccggatc gaaatctcac acacttgtat
      481 agcagcttgc tcgaatatgc gtttttgacg cgcgagcaga tctctgcaat gccggtggaa
      541 gcaccggacc tggaaaccgc cgccaaacaa tatgtggaaa ctctcaaaaa gactgcgggc
      601 tcgccaccgg tgctcgacct tgtccacctg ggtctcggac ccgatggcca cacggcctcg
      661 cttgtgcctg gagatgcggt tctgaatgtc actgacagag acgttgcgat cacaggagtc
      721 taccaaggca ggcgccgaat gacattgaca tatccgatca tcaaccgtgc ccacggtatc
      781 ctatggctcg tgaccggaga cgacaaagtt tcggcgctcg cgcggttgcg cgagggcgat
      841 ccgacgattc cagccggcgg ggttcagcgc gacagcgccc tggttctcgc cgaccaggca
      901 gcggcgccga ccgagaagta actttaccgc ccagccgctg atactccgag taaagaggtt
      961 ccttgctcaa gaagacccga gatctcggtg aaatctagcc gcgcttttcc gactaaaact
     1021 actctagtca tcattcagct cgtcattatt ggttatggga gaatccgtac tcagagtagc
     1081 cctatctgca cagttgaaaa tcgctctgcg atacttattt tttgttggtt tccgtttgca
     1141 acggttttgc aggtgaccct agaatgaaat cgtttagcgc atctaaagca gaaataggag
     1201 acatcatgga atttagatgt gataactcga caggccaatg gcactgttgc acaaattgtt
     1261 cgaagtggcc ggaacaatcg ttcgacatcg tttggatgga acatcttccg agtcgctttg
     1321 cactttgtga caaatgcaaa aatctaacgg aaggcgcccg ctgcaccgat gagtcactcg
     1381 tggacgtcgt ccctgctaca gcccaataac tatcccacgt ttacgcgaaa cgttggtcac
     1441 tcgatttgaa ccactgattg tccgctgcct agaaacgatc acaaaagcag caggcagagc
     1501 tttggttgca ttttccgcgg aatagatcga agcgctggag taccgccgtg aatagattag
     1561 acgcggaatg tcccgaaaag ctttctcttg caggtggagt ttaagatccg ctcaaacgag
     1621 ttttccgcaa cgttaggaaa ctctccttaa ggaatcgcga tcgcgcgctc tcggcgtgct
     1681 gcatcggcac tccagattcc agaaccctgc gtcgccatat acaagaaaat gaaacaaaat
     1741 aagaccgcgg gttccccggc gttttccaga ggccaaaaac tttttggaaa gtgaccaata
     1801 aaataagcga ccgccatttc gccgctggcg ataaatgccg cgtagctcgt gaagaggcca
     1861 atggtaatca gcacgcccag gatcaattcg attactccag ccacaccgaa caatgaagcg
     1921 atcggcaccg ggtcgccgcc gaaccagccg aaaagtttct gtccggaatg gcaaatgaac
     1981 gaaaggccgg caatgattcg caaaatggcg tacgcatacg gggcaaatgc agtaagagcc
     2041 ttatccacag caaatctcct cgctaagagt catgactgat gtgacacact tgctagcgac
     2101 ctcgctcttg tgatgcagcc ggttcgctcc gccatcgtcg gtggctataa ggacgtttct
     2161 aggcctctgg tcattagatt agatgacgag cgcttgcgcc tccattagcg acgggcaaca
     2221 tcgccgtgtc caaggccggc gcacggggca atcctgtgac atcgagaatc tcctgctcgt
     2281 tgagggtttc ccgcgcgacc aacgcctggg cgagcgcgtc cagttgcttg cggtgctcta
     2341 tcaatagttg cttcgcctga tcgtggcttt cagtgatgat tttgagcacc tccgagtcaa
     2401 taatctccgc ggtttcgtcg ctgaacggct tgctggttcc aaagtcggcc tggccgttca
     2461 ggtacggatt gtctcgcggc gccaattgta ccaacccgag tcgttcactc atcccccagc
     2521 gcgtcaccat ccgccgcgcc agcccggtgg cttgctcgat atcgctttcg gccccagtcg
     2581 ttttagtgcc gtagacaatc tcctccgccg cacggccgcc gagcatgccg acgatgcgcg
     2641 cccgcaggta ggcctcggga tagttgtagc gatcgctgtc agggcgctga taggtgacgc
     2701 cgagcgcctg gccacgcggc acgatggtca cgcgattgac cgggtctgca cccggcacca
     2761 ccagccctaa aatggcgtgg ccgccctcgt ggtacgcgat gcgttcttta tcggcgggac
     2821 tcaaaagaag cgggcgctcg gggccgagca caatcttctc gagtgagtcg aggaagtctt
     2881 tttgccgaac ttcgttctgt tcgcgccgtg ccgcgagcaa cgccgcctca ttgacgagat
     2941 tcttcaaatc ggcgccggag aatccaggtg ttgcggcggc aatctcttct agacttacat
     3001 ccttcgccag cggcaccgtg cgcgtatgga ctttaagaat tgcctcacgt ccatttttat
     3061 caggcagatt aacgatgacg cgccgatcga accgtccggg tcgtagcagc gctttgtcga
     3121 gcacgtcagg ctgatttgtc gctgccaaga cgatgatgcc ctcgcggctc gagaagccgt
     3181 ccatttcagt caggatctga ttgagcgtct gctcctgctc gctggaaccg ccgatcgcca
     3241 cctggccccg cgcgcggccg atcgcatcga gctcgtcgat gaaaacgatc gcgggcgcat
     3301 tctcgcgcgc ctgcttgaaa agatcgcgca ccctagctgc gccgacgccg acgatcatct
     3361 cgacaaactc cgccgcgctc atggaaaaga acggcacacc ggcttctccc gctacggcct
     3421 tggcgagcaa ggttttcccg gtgcccggcg caccgatcaa cagcactcct ttgggagcgg
     3481 tgccgcccag gcgggtgtac tttttcgggt ctctgagaaa atccacgatt tcgatcagct
     3541 cgttttccgc ttcgtcgatg ccagcgacat cgtcgaacgt cactttcgta tccttctcct
     3601 ggtcatagcg ccgcgccctg ctttttccga tccccatgag cccgccgccc aaaccgccgc
     3661 cctgctgtgc gcggcggaac atccagacat aaaatccaat gaagagcagc gccggaccaa
     3721 atccaaagag tagggtggat atcagcccgc cgccttcctc aattggcttg gcgctgatct
     3781 caacgccatt attgattaag aatccctcca acccgggacc cacgaagctg ggcaatgtcg
     3841 tggtgaaatt gggcgaagtt ttgggaacag tacgcgttag accagatggg cttggctttt
     3901 caggtttgct gctcgatgcg gcggtttttt gttcggcagg cgggtacgtc accggggagg
     3961 cgaaacggcc agtgatggac tcaccttggc tatagatcga cttgacgttg tgctttgcga
     4021 cctcctcctt aaaaaaggtg tagggaattg tgaccggcgc ttccgggcct ggaataagaa
     4081 atcttacaat caaataattt gccaccagca cgattaagaa
//
LOCUS       DASULZ010000693         3472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_383047, whole genome shotgun sequence.
ACCESSION   DASULZ010000693 DASULZ010000000
VERSION     DASULZ010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383047"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="VFY96_14345"
     CDS             <1..915
                     /locus_tag="VFY96_14345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX5607701.1"
                     /translation="IARFGYRSLLSVPLLREQQILGGLTVWRRQTGEFKTEVINLLQT
                     FATQSALAIHNARLFREIEEKSHQIEAANRHKSEFLANMSHELRTPLNAIIGFSEVLQ
                     ERLFGELNEKQAEYTSDILTSGQHLLSLINEILDLSKVEAGRIELELAPFDLPLAIEN
                     ARTFVRERAVKHGIALDVDLDERLGEYVGDERKIKQILLNLLSNAVKFTPEGGRISIT
                     ANKTDTAVEISVSDTGIGIASEDQPKIFEEFRQVGGDYAHKKEGTGLGLTLAKKFVEL
                     HGGKIWVQSEVGKGSRFTFTLPERLTSH"
     gene            974..1495
                     /locus_tag="VFY96_14350"
     CDS             974..1495
                     /locus_tag="VFY96_14350"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5607702.1"
                     /translation="MIILEKPTTRRQQEFLAAVERSKRLHASWVTAPQTSAAFKAYLQ
                     RTQREAHLSYWVITEDHALAGVINVNEIVRGAFCSGYLGYYAFMPHNGKGYMSRGLGT
                     VLRNVFRAHGLHRVEANIQPGNDDSRRLVERLGFRLEGFSPRYLKIAGRWRDHERWAL
                     TAEDWKDNARKRG"
     gene            1545..1874
                     /locus_tag="VFY96_14355"
     CDS             1545..1874
                     /locus_tag="VFY96_14355"
                     /inference="COORDINATES: protein motif:HMM:NF014074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607703.1"
                     /translation="MHYATSGATLISLAAGEIVYGEHAVLGLVDPYLGKFPAASMLKV
                     LGRKTPKDKDMEDETLIIADQAEKPIVQVRESVKDLLQGKPTDGAEDELALLLSEGTW
                     THDFRLL"
     gene            2465..2791
                     /locus_tag="VFY96_14360"
     CDS             2465..2791
                     /locus_tag="VFY96_14360"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX5607704.1"
                     /translation="MKTNGSLSLGITFSILPFVCACQTMRSEAIVKVPTVENSQDEAL
                     SQSVREKLANASKVNLGAVNVISSNGTVYLTGTVTSLDERQQAAKIAWNAPGVATVVN
                     TLQVKK"
     gene            complement(2859..3257)
                     /locus_tag="VFY96_14365"
     CDS             complement(2859..3257)
                     /locus_tag="VFY96_14365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5607705.1"
                     /translation="MLKTLHMSQPRILVVEDNPRARELLVLLLEREHYEVMEAASGAE
                     ALDRVTGEEFDLIFLDLGLPIITGDEVVRRIKSDPCTARIPIIVNTAFDRDASIVKDA
                     IAAGADQVLYKPADFQNFVPVARRFVDKRS"
BASE COUNT          839 a          911 c          925 g          797 t
ORIGIN      
        1 attgctcgct tcggttaccg ctcgcttttg tcagtgccgc tacttcgcga gcaacagatt
       61 ctcggtggct taacggtgtg gcgacgtcag accggggaat tcaaaaccga ggtaattaat
      121 ctgttgcaaa cctttgcgac ccaatccgct ttggcaattc acaatgcgcg actgttccgt
      181 gagatcgagg agaagagcca ccagatcgaa gccgccaacc ggcacaagtc agaatttctc
      241 gccaacatgt cccacgagct taggacgccg ctcaacgcca tcatcggctt ctcggaagtc
      301 ctgcaggagc gactcttcgg agaattaaac gagaaacaag ccgagtacac ctcggatatt
      361 ctcacatccg gtcagcatct gctgtcgctc atcaatgaga ttctcgatct ctccaaagtc
      421 gaggcaggcc ggatcgaact ggagctagct ccttttgatc tgccgctggc gatcgaaaat
      481 gcacggacgt ttgtgcgcga gcgcgccgtc aagcacggta ttgcgctgga tgttgatcta
      541 gacgaacggc ttggggaata cgtcggcgat gagcgtaaga tcaagcagat tcttctcaat
      601 ctattatcca acgccgtgaa gttcaccccc gagggaggac ggatcagcat cacggccaat
      661 aagacagaca ccgctgtgga gatctctgtc agtgacaccg gcatcggcat cgcttccgag
      721 gatcagccga aaatcttcga ggagtttcgc caggtgggag gcgattatgc tcacaagaag
      781 gaaggaaccg ggctcgggct gactttggcc aagaagtttg ttgagctgca tggaggaaag
      841 atctgggtgc agagtgaggt cggcaagggc tccagattta ccttcaccct gcccgaaagg
      901 ctgacgtcac attgacttga cgagactggc agcgcgcagt tgagaaggaa accatgttca
      961 ctgccgaaac gagatgatca tacttgagaa gccaaccacg cggcgacagc aggaatttct
     1021 agcagccgtg gagaggagca agcgccttca cgcatcgtgg gtgactgctc cgcaaacctc
     1081 agccgcattc aaagcttatc tacagcgaac ccaacgtgaa gctcacctga gttactgggt
     1141 gattactgaa gatcacgcac ttgcaggcgt catcaacgtt aatgaaatag ttcgtggtgc
     1201 gttttgttcg gggtatctcg gctactatgc gttcatgcca cataatggca aagggtatat
     1261 gagccgaggt ttgggaacgg tcctgagaaa tgtctttcgg gcgcatggcc tacaccgtgt
     1321 tgaagcaaac attcagcctg gcaacgacga ctctcgacgg ctagttgagc gacttggttt
     1381 tcgtctcgaa ggcttttctc cgcgttatct taaaatcgcc ggccgatggc gcgatcatga
     1441 acgatgggcg cttacggcag aggactggaa agacaacgcg cgcaagcgcg gataaggatc
     1501 ggctctttgg agagccacgg tcggcttcac gccgccgctc ctggatgcat tatgcgacgt
     1561 cgggcgcgac actgatttca ctggccgccg gcgagatcgt ttatggcgag catgccgtgc
     1621 tcgggctggt ggatccttac ctgggcaagt tcccggcggc gtcaatgctt aaagtgcttg
     1681 gtcgaaaaac gccgaaagat aaagatatgg aggacgaaac tctgataatc gctgaccagg
     1741 cggagaaacc gattgtccag gtccgcgagt ccgtgaaaga cctgctgcaa ggcaaaccca
     1801 ccgacggcgc agaggatgag ctcgcactcc ttctttctga aggtacctgg acgcacgatt
     1861 tccgattact ttgaaacagc caaagcgctc cgcttgaccg tcagttccaa ttataccccg
     1921 acgacgttct gacgggtcat gagcctttat ccccagccca cacgcactca gccgacagta
     1981 cctgtgccgc gcactcgcag acccgagcta gtgccagcgg cgaaccagtg atgccttcgt
     2041 ctcaccatcg ggaacgggcg caggagccca cctgatacta cccgctcgga gtcgctgacg
     2101 actgataaat gtacgacaaa tactattttg actgaactaa tttccttcag gtcacgaagt
     2161 atcgcggttc aaattaccgg tccacgttct gccattgtgc cgtgttagcg ccctcttata
     2221 cgatctcgtg cctaaccggc attgctgttg ctgctctgat ggtagggggt gggcaaatgg
     2281 aaacagcaac catactatcc cgcgccgagc cttccagctc aggggtggcg ttcaattggc
     2341 aatttttagt tttctgggta ctctatttcg ctgtaatagt gaggatcgta tggtcattgg
     2401 ttacgcatgt actttgaggt gaaagatcaa ccgatgactc gtcccatcca gtaatggaga
     2461 aaacatgaaa actaacggta gcctttccct cggcataaca ttctcaattc tgccgttcgt
     2521 ttgcgcttgt caaacaatga gaagcgaagc aattgtgaag gttccgacgg tagaaaattc
     2581 ccaagatgaa gcgctgtcgc aatcggtccg tgaaaaactc gcaaacgcca gcaaagtaaa
     2641 tttgggcgcg gttaatgtta tatccagtaa cggcacagtg tacctgacgg gaactgtgac
     2701 gtcgctcgat gagcgtcagc aagcagccaa aatcgcatgg aacgcgccgg gcgttgcaac
     2761 tgtcgtgaac acgctgcagg tgaaaaaata agagtcacct agttctggtc gccggctagc
     2821 cttgctccca cggtcttagg ggtgtttggc tcaaacgact aggatctctt atcaacgaat
     2881 cttcgagcga ctggaacgaa attttgaaaa tcggctggtt tatataaaac ctgatcggct
     2941 cccgcggcaa tagcatcttt tacgatgctc gcgtcccgat cgaaggcggt gttcacgatg
     3001 atcggaatgc gagcagtgca gggatctgac ttgattcgcc gtaccacctc gtcgccggtg
     3061 attataggca aaccgagatc caaaaaaatc agatcaaatt cctcgccggt gactcgatcg
     3121 agcgcttcag caccactggc cgcttccatc acctcataat gttcacgctc taaaaggagc
     3181 actagaagct ctcgggcacg aggattgtct tccacgacca gtattcttgg ttggctcatg
     3241 tgaagcgtct taagcatttc ttgtaccggc cacttacact catttctttt gaaggttcac
     3301 agttccgaag aggttagtat tctcaggaaa gctgctcagc tcggcggtac tgaggattag
     3361 attaaccgcg gcgaattctt cgcgacgtgc ggctttttac tagctgtagt gagtcggcgc
     3421 tctcacgacc tttttaaagt acccatcgca tacttcaatg ctctcttagc gt
//
LOCUS       DASULZ010000694         2127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_789376, whole genome shotgun sequence.
ACCESSION   DASULZ010000694 DASULZ010000000
VERSION     DASULZ010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789376"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VFY96_14370"
     CDS             complement(<1..353)
                     /locus_tag="VFY96_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013818103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase"
                     /protein_id="HEX5607706.1"
                     /translation="MRGVGLIAGLILVVSACGIQRYDLADCTTYVAAGFRKIADERAQ
                     RFLGKVQEDTARCRGGDRAVAFRSMPWVDWQNYWAAGDSATKASGPSGADVHVSPNGR
                     GVDGALLDLEYQRIEL"
     gene            complement(391..1140)
                     /locus_tag="VFY96_14375"
     CDS             complement(391..1140)
                     /locus_tag="VFY96_14375"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX5607707.1"
                     /translation="MRMTLNGKKAIVERKAALIKLLACFMALVALSLAWRLTPLREII
                     DFETIVGWQQSIKNYPAVFLWVVGIYVVSSLILFPTTLLNVATVVTFGPIVGNLYAFA
                     GWLSSATIGFFIGRLFGRNGVRKIARTGMEHIFDSATRHGFLTVFSTRLVPIAPFTVV
                     NVIIGASEIGFSVFLLATAAGRIPGIITITLFGVQLEMFLRRQDFVSWVMLGATMVVV
                     VVGSAWCTKRFTAPRPGRKTSQITDETPGYY"
     gene            1338..1508
                     /locus_tag="VFY96_14380"
     CDS             1338..1508
                     /locus_tag="VFY96_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607708.1"
                     /translation="MKFSRWLRRALLIITLYTIGLVEAYYLPRYGIEWLLGALGATAF
                     LAGFWMRAHRDI"
     gene            complement(1621..>2127)
                     /locus_tag="VFY96_14385"
     CDS             complement(1621..>2127)
                     /locus_tag="VFY96_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607709.1"
                     /translation="NRAGVQIDLIVRGMCSLRPGLEGVSERIRVISIVDRYLEHARVF
                     YFQNGSAPTFWLASADWMPRNFKRRIEIAFPVLDPQLQAKLKDILELQLVDSVKAWHM
                     EPDGSYVRKPSVNGPGFRFQERFYEMLRAEEISRSSRGMAMDGPNFRSADDDLTAMHQ
                     WWEENASK"
BASE COUNT          488 a          627 c          529 g          483 t
ORIGIN      
        1 agctcgatgc gctgatattc gagatcgagg agagctccat ccacgcccct gccgtttggg
       61 ctcacatgaa catctgcgcc actcgggccg gaagctttag ttgcgctgtc acccgctgcc
      121 caatagtttt gccagtccac ccacggcatt gagcgaaaag ccacggcgcg atcgccgccg
      181 cggcagcgcg cggtgtcctc ttgaactttg ccgaggaatc tctgcgcgcg ttcatctgca
      241 attttacgaa atccggcggc cacatacgtc gtgcaatccg ccagatcgta tctctgtatg
      301 ccgcaggcgc taacaaccag gatcagccct gcgatcaaac cgactccgcg cactcgccag
      361 cttccctttg atagaggcga tctcggtgaa ctaataatag ccaggtgtct cgtcggtgat
      421 ttgcgaagtt tttcgaccgg ggcgcggcgc agtgaacctc ttggtgcacc aggccgatcc
      481 gacaacaacc acgaccattg ttgctcccag catcacccaa ctgacgaaat cttggcggcg
      541 tagaaacatt tccagctgca caccgaacaa cgtgatcgtg ataatgcctg ggattcggcc
      601 ggccgcagtg gccagcaaaa aaacgctgaa accaatttcc gaggccccga tgataacgtt
      661 cactaccgta aacggtgcga tcggcacaag acgagtactg aacacggtta aaaacccatg
      721 tcgagtagcg ctgtcaaata tatgttccat tcccgttcgg gcgatttttc gtaccccatt
      781 gcggccgaac agccgtccga taaaaaaacc gatcgtggcg ctactgagcc atcctgcgaa
      841 ggcatataga tttcctacga tcggaccgaa cgttactacg gttgcgacat ttagcagcgt
      901 tgttggaaac agaatcagac tgctcactac atagattccc acaacccaga gaaacaccgc
      961 cgggtaattt tttatcgatt gctgccagcc gacaatcgtc tcgaagtcga tgatttctct
     1021 gagcggagtt agacgccacg cgagcgagag cgcaaccagc gccatgaaac aggccaacag
     1081 cttgatcaaa gctgccttgc gctcaacgat tgcttttttt ccatttagag tcatgcgcat
     1141 agagcgtcac tgacgtttac gaaccggcgt ctctgagctt aaattcgaag gagctcaacg
     1201 aatcaaacaa cgtacaaccc tcatccgcag aacaattttg gacaggctca ccgtcaggtt
     1261 ttgtcacaaa aagcgcgtcg tcgtgccacg tcttgcgcct ttgcatcctg gtataaatca
     1321 tgccctacca agctttcatg aagttttccc gatggctgcg gcgcgctctg ctgattataa
     1381 cgctttatac cattggactg gtcgaagcct attatttgcc gcgctacggc atcgaatggc
     1441 tcctgggcgc gctgggtgca acggcctttc tggccggctt ctggatgcgc gcgcaccggg
     1501 atatttaacc tgccagttca catcgtcgag gctcgcgtcc ggctcacgct gtgtattgtt
     1561 cagagccggt tacaccttcg tcggctcgcc gcgacgctct ctacatacga tcgatgagag
     1621 ttattttgac gcattttcct cccaccactg gtgcatcgca gtaaggtcgt catctgcgct
     1681 gcgaaaattc ggtccgtcca tcgccatgcc tcttgaagac ctcgagatct cttccgctcg
     1741 aagcatttca taaaagcgct cttgaaaacg gaaacccggt ccattgacgc ttggttttcg
     1801 tacgtacgag ccgtccggct ccatgtgcca agctttgacc gagtcgacaa gttgaagctc
     1861 taaaatatct tttagtttgg cctgcaactg cggatcgaga accgggaagg cgatttcgat
     1921 gcgccgtttg aaattgcgcg gcatccagtc cgccgaggcg agccaaaacg ttggcgcgct
     1981 gccgttctgg aaataaaaca ctcgggcgtg ctcgagatag cgatcgacaa tcgagatcac
     2041 tcgtatacgc tccgaaactc cctccagccc tggccgcaga ctgcacatac cgcgcacgat
     2101 gagatcgatt tgcacacccg cccggtt
//
LOCUS       DASULZ010000695         2180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_992979, whole genome shotgun sequence.
ACCESSION   DASULZ010000695 DASULZ010000000
VERSION     DASULZ010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992979"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VFY96_14390"
     CDS             <1..708
                     /locus_tag="VFY96_14390"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5607710.1"
                     /translation="KLREQIAAAFQEKFALPILEGYGCTELAPVVSVNIPNVGFGKEA
                     QIGNKRGTVGRPIPGVSVKVVHPDTFQPLPCGQEGLLLVKGPNVMLGYLNQPALTQEA
                     IRDGWYVTGDIAALDEDGFVRITDRVSRFSKIGGEMVPHMRVEEVINNILGSAASVVT
                     AVPDSHRGEKLIALYCQNGITTEELWQRLNDSDLPKLWIPKRDDLFAIDSLPVLGSGK
                     IDLKAAKNLAREHASKE"
     gene            869..1849
                     /gene="bioB"
                     /locus_tag="VFY96_14395"
     CDS             869..1849
                     /gene="bioB"
                     /locus_tag="VFY96_14395"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015186836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEX5607711.1"
                     /translation="MDFHQLADKALRSTIPERAELKAVLEASEEQLPELLSAAFKVRR
                     HYYGKRVQIHVLQNAKSGLCPEDCHYCSQSSVSEAPIERYKFMSKETLVERARQAKAA
                     GAVRFCIVNSGRGPTAKEIDEIAEAVREIRAETGMNICCSLGLMTEDKVHKLKEVGVG
                     RINHNLNTSRAHHPEIVTTHTYEDRVATIESVKKAGVSTCSGGIIGMGESDDDIIDLA
                     LSLRAMDIDSIPVNFLTSIAHTPFENKHELTPQRCLKALCLFRFVNPSKEIRVAGGRE
                     INLRSLQPLALYPANSIFVNGYLTTPGQEASDAHQMIRDLGFELDSPAAI"
     gene            2012..2143
                     /locus_tag="VFY96_14400"
     CDS             2012..2143
                     /locus_tag="VFY96_14400"
                     /inference="COORDINATES: protein motif:HMM:NF025088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607712.1"
                     /translation="MHVEQEDTIAKFWLDPIRLQRSADFLGLKLAALKVLFQPTTSD"
BASE COUNT          529 a          616 c          583 g          452 t
ORIGIN      
        1 aagctgcgcg aacaaatcgc ggctgcattt caagaaaaat tcgctctgcc gattctggag
       61 ggttacggct gcaccgagct agcaccggtc gtctcggtaa acatacccaa cgtcggattc
      121 ggcaaagagg ctcagatcgg caataaacgc ggcaccgtcg gccgtccgat ccctggcgta
      181 tcggtgaaag tcgtgcaccc ggacacgttc cagcccctcc cttgcggcca agaaggcttg
      241 ctgctggtca aagggccgaa tgtaatgctc ggttatctca atcaacctgc tctaacccaa
      301 gaagcgattc gcgacggctg gtatgtgacc ggcgacatcg cggccctgga cgaagacgga
      361 tttgttcgaa tcaccgatcg ggtttcccgc tttagcaaga tcggcggcga aatggtgccg
      421 cacatgagag tcgaggaagt gatcaataac attcttggca gcgccgcctc ggtggtgacc
      481 gcagttcccg attcgcatcg cggcgaaaaa ctgatcgcgc tttactgtca gaacggaatt
      541 acgacggaag agctctggca gcggctcaat gactccgacc tgccgaaact ctggattccc
      601 aagcgcgacg acctgttcgc gatcgattca cttcccgtgc tgggatcggg aaagatcgat
      661 ttgaaagcgg ccaagaatct cgcacgggaa cacgcaagca aagagtgacg tctcccgatt
      721 acgttcaaac ccatagcata gagatcaact ttgatctcgc ggttgattga cagctggact
      781 ccgcgtgtct gcccgagctc cagaatacgg aagattattt gcaagctctc ctgacgcatg
      841 ctaaaaactt gctgactcgg aggtagtaat ggattttcat cagctcgcgg acaaagcact
      901 tcgaagcacc atccccgaac gggcggagtt aaaagccgtc ctggaagcga gcgaagagca
      961 attgccggag cttttgagcg cagcgtttaa ggtgcgccgt cattattacg gcaagcgtgt
     1021 gcaaatccac gttttgcaga acgccaagag cggtctctgt cccgaagact gccattactg
     1081 ttcgcagtcg tcggtctccg aagcgcccat cgagcgttat aaattcatgt ccaaagaaac
     1141 tctcgtcgaa cgagcgcgac aggcgaaggc ggccggcgcg gtgcgcttct gtatcgtcaa
     1201 cagcggccgc ggtcccaccg caaaagaaat cgacgaaatc gccgaggcgg tgcgcgagat
     1261 ccgcgctgaa acgggcatga acatctgctg ttcactcggc ctcatgaccg aagacaaggt
     1321 tcacaagctc aaagaagtcg gtgttggacg catcaatcac aatctcaata ccagccgcgc
     1381 ccatcacccc gagatcgtca ccacgcatac ttatgaagac cgagtagcga ccatcgagag
     1441 cgtaaaaaaa gccggcgtca gcacctgctc aggcggcatc atcggcatgg gtgagagcga
     1501 cgacgacatt atcgatctcg ctttgtcgct tcgcgccatg gatatcgatt cgatcccggt
     1561 caattttttg acctcgattg cccatacgcc tttcgagaat aaacacgagc tcacgccgca
     1621 acgttgcctg aaagcccttt gcctatttcg cttcgtcaat cccagcaaag aaatccgcgt
     1681 cgccggcggg agagaaatca acctgcgctc gcttcagccc ctcgcgctct atccggccaa
     1741 ttcgattttc gtcaacggtt atctcaccac tccggggcag gaagcttccg acgcacatca
     1801 gatgatccgg gatctcggct tcgagctcga ctcaccggca gcgatctagg tcgccgtagc
     1861 gccctcagtt cgtcacaatg agaagccaag tggggggagt gcgatcataa actgcacgac
     1921 gtactactaa gcagtcggtg atgccaacag ttctacggca tggtccattt cgttttttct
     1981 tgacgccggc gatcgggacg aaccacccca tgtgcacgtc gaacaggaag atacgattgc
     2041 aaagttctgg ctagacccca tcagattgca acggagtgcg gattttctcg ggttgaaatt
     2101 ggccgcattg aaagtattat ttcagccaac cacgtccgat tgatggaggc atggaatgag
     2161 tatttcggcg gttgaaatca
//
LOCUS       DASULZ010000696         4445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_993011, whole genome shotgun sequence.
ACCESSION   DASULZ010000696 DASULZ010000000
VERSION     DASULZ010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993011"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VFY96_14405"
     CDS             complement(<1..625)
                     /locus_tag="VFY96_14405"
                     /EC_number="1.17.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin/ferredoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX5607713.1"
                     /translation="MERRKTRQIQLGKVKIGGDAPITVQSMTKTDTRDVRATVQQIWD
                     LEAAGCEIVRCAVPVREAAEQLGEIKRKIRIPLIADIHFNYKLALIAIEQGVDGLRLN
                     PGNIGARKYVEEVVKAASARRIPIRIGVNAGSLEKDLLQRYNGPTAEGMVESGLRHIR
                     ILEDLGYHEIKISLKASDPLMMIEAYRLLADQVDYPFHLGVTEAGTPG"
     gene            complement(704..1507)
                     /gene="jag"
                     /locus_tag="VFY96_14410"
     CDS             complement(704..1507)
                     /gene="jag"
                     /locus_tag="VFY96_14410"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HEX5607714.1"
                     /translation="MDFVETEGENIDDAIENALKLLGVGRDKITVDIIAEGRKGILGF
                     GAQKARVRAQLRTPRLGQPSEPEAATSEPAGFSAEAAALVQKAETALREILRLMGVQA
                     TIEIKTSDDETTLQIHADNSGLLIGRKGQTLEALQYLVTRIAGEHYAAEGPHIVVDIE
                     KYRERRRKMLEDMALRLGEKAKRQRKTVTVEALSAADRRIIHAALQDDPWVSTKSLGQ
                     GSYRRLLIIPEGDRKKKDTAASPQPQKQPERAQSKTEGEPISADDEDSQ"
     gene            complement(1534..3162)
                     /gene="yidC"
                     /locus_tag="VFY96_14415"
     CDS             complement(1534..3162)
                     /gene="yidC"
                     /locus_tag="VFY96_14415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX5607715.1"
                     /translation="MDKRALIGIGLSILVLVVYQQVITYFYGPPPTVSAPEMEKSKPE
                     IAEPRQDNPPIASSVAPATPEAAVAAAPAKEVTVETDNYIAVFTTAGARLKSFKFKKY
                     RSAVDEKSPPFEMIRNVPGVPLPLGVRWQATSPVDDSEVIYSVQGADLKVSGDNKGSL
                     VFRGQAKNGAVITKSFTFFGATYPIQMEVSIKGADGAAPSPEIVLADQSDHSAPNHDA
                     PFEGFIALVDNKIRREAPAEAAKGHEFTGDVSWAGFGHTYFLFALLPENGAQHKVSVR
                     QTGAALIGAIGGQAATQHYTVFIGPKEIDTLKAVGKGIERSIDFGIFWFISIPLLYIL
                     HFFHRFTASYGVDIIILTVLIKVLMAPLTHKSYVSMKQMQKLQPQMERLKERYNEDKE
                     KLNKEIMELYRRNGVNPLGGCLPMVLQLPVFYGLYNCLGTPIELRHAPFLWIKDLSRP
                     DWQSLAFSVPSWWPSFLPAGIPVLTILMGASMFVQQWMTPTAGDPNQRKMMMLMPLMF
                     TFMFVSFPAGLTVYWLVNNLLTIGQQYWINRSVK"
     gene            complement(3174..3386)
                     /gene="yidD"
                     /locus_tag="VFY96_14420"
     CDS             complement(3174..3386)
                     /gene="yidD"
                     /locus_tag="VFY96_14420"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX5607716.1"
                     /translation="MSRTVFCWLIFAYRNFISPLLPRACRFYPTCSVYTQQAIQRYGI
                     VQGVAYGINRIARCHPWHPGGYDPVK"
     gene            complement(3383..3763)
                     /gene="rnpA"
                     /locus_tag="VFY96_14425"
     CDS             complement(3383..3763)
                     /gene="rnpA"
                     /locus_tag="VFY96_14425"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX5607717.1"
                     /translation="MESLTRSQRFPKTARLRKRAEFVNLSRRGVKLQSANFVVVVWAK
                     GQQDNRLGITVSGKVGNAVTRNRIKRSIREYFRRHRAELPMESDILIIARRGAAELDG
                     HGVRVELEKIFAAQRKRQVLKFIR"
     gene            complement(3770..3931)
                     /gene="rpmH"
                     /locus_tag="VFY96_14430"
     CDS             complement(3770..3931)
                     /gene="rpmH"
                     /locus_tag="VFY96_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX5607718.1"
                     /translation="MKRTYQPSNIRRKRTHGFLARMETPGGRNVLKRRRAKGRKRLTV
                     TVPPKRART"
     gene            complement(4005..>4445)
                     /locus_tag="VFY96_14435"
     CDS             complement(4005..>4445)
                     /locus_tag="VFY96_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005987312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX5607719.1"
                     /translation="GVIKNVIAIAAGISDGLSLGQNARAALITRGLAEMTRLAVRMGA
                     DPLTLAGLPGLGDLVLTCAGDLSRNRAVGLQIARGKPLHEITTATRTIAEGVRNTRTV
                     CLLAERLQVDMPIVEQMAQVLYGGKRPASAVRDLMLRSLKAEAG"
BASE COUNT          935 a         1280 c         1172 g         1058 t
ORIGIN      
        1 tacccggagt tccggcctcc gttacgccaa gatgaaacgg ataatccacc tgatcggcaa
       61 gcaatcggta ggcttctatc atcatcagcg ggtcggatgc tttaagcgag atcttgattt
      121 cgtggtagcc gagatcttcg agaattcgaa tgtgacgcag cccgctttcg accattcctt
      181 cggcggtggg cccgttgtag cgttgcagca gatccttttc cagtgacccg gcattgacgc
      241 cgatacgaat gggtattctg cgcgcagagg cagctttgac aacctcttcg acatacttac
      301 gcgcgccgat attacctgga ttgagccgca gaccgtcaac gccttgctca atggcaatca
      361 gcgcgagctt ataattgaaa tgaatatcgg cgatcagtgg aatgcgaatt tttctcttga
      421 tctcgccgag ctgctctgcc gcctcgcgca ctggcactgc gcagcggaca atctcacaac
      481 ccgccgcctc gagatcccaa atttgctgga ccgtcgcgcg cacatcgcga gtgtcggtct
      541 tggtcatcga ctggacagtg atgggagcat caccgccgat tttcaccttg ccgagctgaa
      601 tttgtctggt tttgcgccgt tcgatcacgt ctctactata gagaaccgat gccggggggt
      661 caacaaacgg acggcggcgc gggtttgaga gccttcgctg ctgttactga gagtcttcgt
      721 cgtccgcaga gatcggctct ccttcggttt tgctttgcgc tcgttcgggc tgcttttgcg
      781 gctgcggcga agccgccgta tccttttttt tgcggtcccc ttccgggatg atcagcagcc
      841 gccgatacga accctggccg aggcttttgg tcgacaccca aggatcgtcc tgcagagcgg
      901 cgtgaataat acgacggtcg gctgcgctca gcgcctcgac cgtcaccgtt ttacgctggc
      961 gtttagcttt ttcacccaag cgcaacgcca tgtcctcgag cattttgcgc cgccgctcgc
     1021 ggtatttctc gatgtcgaca acgatatgcg gtccctcggc ggcgtaatgt tcaccggcaa
     1081 tacgggtcac aaggtattgt aacgcttcca gggtctgacc tttgcgaccg atcagtagtc
     1141 cgctgttatc cgcatggatc tggagtgtgg tctcgtcgtc ggaggttttg atctcgatcg
     1201 tcgcctgcac acccatgaga cgtaggattt cccgcaacgc cgtttccgcc ttttgcacca
     1261 gagcagccgc ttcggcgcta aaaccagccg gctcgctcgt cgccgcttcc ggttccgatg
     1321 gctggccgag ccgcggtgtt ctcagctgag cccgtacccg cgccttctgc gcaccaaacc
     1381 cgaggatccc cttgcgccct tcggcgatga tatcaacggt gattttatcg cggccgacgc
     1441 cgagcaattt gagcgcgttt tcgatggcgt cgtctatgtt ctcgccttcg gtctccacga
     1501 aatccataaa ttctgcctct caacgaaccg atctcacttg acactgcggt tgatccaata
     1561 ctgctgaccg atggtaagca gattgttgac cagccagtaa accgtcaatc ccgccgggaa
     1621 cgacacgaac atgaaagtga acatcaaagg catcaacatc atcattttcc tttgattggg
     1681 atctcccgcc gtgggcgtca tccactgctg cacgaacatg ctcgcgccca tcagaatggt
     1741 caacaccgga atcccagcgg gcaagaatga aggccaccaa ctcgggactg agaaagccag
     1801 cgactgccag tcgggccgcg aaagatcttt gatccacaga aacggtgcat gacgaagttc
     1861 aataggcgtc cccaaacagt tgtatagacc ataaaatact ggaagctgca gcaccattgg
     1921 caagcatccg cccagcggat tcactccgtt gcggcgatag agctccatta tttccttgtt
     1981 cagtttttcc ttgtcttcgt tataacgctc cttcaatcgc tccatctgcg gctgaagctt
     2041 ttgcatctgt ttcatcgaga cataactttt gtgcgtcaaa ggcgccatta ggactttgat
     2101 cagcaccgtc aaaatgatga tgtcgacacc gtagctcgcg gtaaaccgat ggaaaaaatg
     2161 caggatgtac agcaaaggaa tcgagataaa ccagaaaata ccgaaatcga tcgagcgctc
     2221 gatacctttg ccgacggctt ttaaggtgtc gatttccttt gggccgataa ataccgtgta
     2281 gtgctgggtc gcggcttgac cgccgatcgc gccgatcaga gctgcaccgg tctgacgcac
     2341 cgagacttta tgctgcgcgc cgttttcagg cagcagggca aacaaaaaat aagtatggcc
     2401 aaatcccgcc caggaaacat cgccggtgaa ttcatgtccc ttggccgctt ccgccggtgc
     2461 ttctcgcctg atcttgttgt ccacgagggc gataaatccc tcgaaaggtg catcatggtt
     2521 gggcgccgag tggtcgcttt gatcggccaa cacaatttcc gggctcggcg cggctccgtc
     2581 tgcgcctttg attgaaactt ccatctgaat cgggtatgtg gcgccgaaaa aggtaaaaga
     2641 ctttgtgatt actgcaccgt tcttggcttg tcctcggaaa accaagcttc ccttgttatc
     2701 acctgatacc ttcagatcgg caccctgcac cgagtagatc acttcgctat cgtccaccgg
     2761 tgatgtcgcc tgccagcgaa ccccgagcgg cagcggcaca ccgggaacat tgcggatcat
     2821 ttcaaaaggc ggactcttct catcgacagc gctccgatac ttcttgaatt tgaaactctt
     2881 cagtcgagca cctgcggtag tgaaaaccgc gatgtaattg tctgtctcca cagtaacttc
     2941 tttggctgga gcagcagcca cagcagcttc tggcgttgca ggagcgacac tcgaagcgat
     3001 gggagggttg tcttgtcgcg gttcggcaat ttccggtttg cttttttcca tttccggcgc
     3061 ggaaactgtc ggcggcggcc cgtaaaaata ggttatcacc tgttggtaaa cgaccagcac
     3121 gagaatcgag agaccaatgc cgatgagcgc gcgtttgtcc atggaaatgc aaactacttt
     3181 actgggtcat agccgcccgg atgccatggg tggcaacgag cgatgcggtt gattccgtaa
     3241 gcgactccct ggacgattcc gtatctttga atggcttgtt gtgtgtatac cgagcaagtg
     3301 gggtaaaatc ggcaggcccg cggcaacaat ggcgagataa aatttcggta tgcgaaaatc
     3361 agccaacaaa aaaccgtgcg gctcatctga tgaatttcag cacttggcgt ttccgctgag
     3421 cggcgaaaat tttctcgagt tcaacgcgca cgccatggcc atcaagctcg gcggcgcccc
     3481 tgcgcgcaat gataagaata tcgctttcca tcggcagttc ggcacgatga cgccgaaaat
     3541 actcgcggat cgagcgcttg atccgatttc gcgttaccgc atttccgact ttgccgctaa
     3601 ctgtgattcc taagcgattg tcctgctgtc ccttcgccca gacgacgaca acgaaatttg
     3661 ccgattgcag cttgacgcca cggcgggaga gattgacgaa ctcagctcgt tttcgcaggc
     3721 gtgccgtttt cgggaaccgc tgcgaccttg tcagcgactc cactcggact tacgtccgcg
     3781 cccgtttggg cggcacagta acggtcaacc gtttgcgtcc tttggcgcgg cgccgtttga
     3841 ggacattgcg accgccgggt gtttccatgc gcgcgagaaa accgtgcgtc cgttttctcc
     3901 tgatattact cggctggtat gttcgtttca tagagtaaat actcccttag gcgcttaaac
     3961 ggccgaatgt ttatatgaac catacccact gagcgttgtc aattttaccc agcctcggct
     4021 ttaagtgagc gcaacatgag atcccgtacg gcgctcgctg gcctttttcc gccatagagc
     4081 acctgcgcca tttgctctac aattggcata tccacctgca gccgctctgc cagaaggcac
     4141 acagtgcggg tattgcgcac gccctcggca atagttcgcg ttgcagtggt gatctcgtgt
     4201 aacggcttgc cgcgtgcgat ctgcaggcca acagctcgat tgcggctcag gtcgccggcg
     4261 caagtaagga ccaaatcacc aagacccggt aatcccgcga gcgttaatgg gtcggctccc
     4321 attctcactg ccaatcgggt catctcggca aggccacggg tgatcagcgc cgcacgcgcg
     4381 ttctgaccca agctcaaacc gtcactaatt cccgcggcaa tggcgattac gttttttatg
     4441 acgcc
//
LOCUS       DASULZ010000697         7387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1093407, whole genome shotgun sequence.
ACCESSION   DASULZ010000697 DASULZ010000000
VERSION     DASULZ010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7387
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093407"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VFY96_14440"
     CDS             <1..930
                     /locus_tag="VFY96_14440"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607720.1"
                     /translation="LWVHEGRAEPVKIAIPSTTQAVLPFTIARDKGYYRAEGLDVELI
                     LVSAPIASRALLSGDVAVATVGGAGLPPVLRGSPFKFLFTTYNRAMFWLFAKPEIRDV
                     RGLKGKRIGVSGIGSGPDSLLREILRQNGLDANRDVAVLSLGVMPTIFAGLQAGTVDA
                     AMLSPPVTFKAEDSGFRELVSFPKQDLVELQGSVLVREASLQSDGPQMERFLRGTYKG
                     FLHIKENRAGTIPFLARYLQLNQELATHAYDQVVRPAMTQDGTLSSEMQAKAVEHVLK
                     RLDLKEAPPLAKIFDFSLTRKVANELQAKNWRP"
     gene            968..1924
                     /locus_tag="VFY96_14445"
     CDS             968..1924
                     /locus_tag="VFY96_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607721.1"
                     /translation="MALKLNMTCALYDRSQPLIDRRVKPRDIDLEIHVNSDDRRRQHE
                     GRNGKFDIVEFFTGIYMADLEHRSLGFTAIPIFVKRMFRHSYIYVNKRVGIREPKDLN
                     GRRVGLQTWFTTTALWARGILEDEYGVDLRSITWLANWHETVGNWQQPSSLKLEIAPP
                     GVKLHDLLVAGKIDAGITTESWAPFGHPDVDFLLPNYAEEERRYYKKTGFFPIQHTLL
                     IRNAVLEQHPWVAMSTFDAWQEAKQECYRWLDRQRVHMTGLWYRALWEEERALAGADP
                     YVWGFKRSRPEIDKMLEYAHRQGLVSGKFRPEDMFHGSTLET"
     gene            1998..3131
                     /locus_tag="VFY96_14450"
     CDS             1998..3131
                     /locus_tag="VFY96_14450"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5607722.1"
                     /translation="MDLSEVVQTGPGTLAGRFLRMFWQPVCCSYELAAGRPLPIRILG
                     EDFTLYRGESGDPHLVGPRCAHRATQLSVGWIEGECIRCFYHGWKYDGSGQCVEQPAE
                     GESFADKIRIPGYPVKEYMGLVFAYLGEGEPPPLPRYPRFETSEVSLDVAGIRRICNY
                     FNNIDNSLDSVHVRFVHQRHRDSVDDHVVLGDPIISVDETQWGVRRYVKYPNGKDVTF
                     FFGMPNINFINGQVVDPVIKRADVMVFKVPVDDESHIHFEVRAIPLTGERGRAWIEQR
                     RRLREQAEQDRPDLVRAILSGKRRLDDIDPNRVDFVMLEDEVAQAGQGAIAVRANEHL
                     GRSDRGVFLLRKIWERELRNLAEGRPLKQWTYRPDMVPTYPKT"
     gene            complement(3196..4188)
                     /locus_tag="VFY96_14455"
     CDS             complement(3196..4188)
                     /locus_tag="VFY96_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEX5607723.1"
                     /translation="MAEKSTKKKSGAARKRRKLAGKSIGLAPIDLLSTDAPRDLEELQ
                     RAVADDGGKVLASYREPFGGHWLLLAALPIEQVEPTPYQRNLSDAHARKLEGVIAKLG
                     RFLDPIIVVRRAGKGSEARYWTPNGNHRLSAMKTLGAKSIIGLVVPETSAAYKILALN
                     TEKAHNLRERALEVIKMYEELAALDGATEETYGLEFEEAALITLGLCYLENGRFSGGA
                     YHPILRRSDQFIKKPLAAALHIRRDRAKALMDLDKTVVSKVEALKSKGLTSPYLKSFV
                     VARINPIRFRPKDATPLGFDDVIERMTKAAEKMNVEKIKVEDLAKSGGAPEEAA"
     gene            4343..5695
                     /gene="gor"
                     /locus_tag="VFY96_14460"
     CDS             4343..5695
                     /gene="gor"
                     /locus_tag="VFY96_14460"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010210355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEX5607724.1"
                     /translation="MAGYDYDLFTIGAGSGGVRASRMSAAFGAKVAVAEERYLGGTCV
                     NVGCIPKKLLVYASHYGEDLHDAAGYGWSAAGQFNWSTLIENKNKEIQRLNEVYRKLL
                     NDAGVTIYEAHAEIVDPHTVSVDGKEITAQYILVAVGSWPVVPKFPGSEYAITSNEAF
                     YLPALPERVVVVGGGYIGVEFAGIFHGLGAKTTQLYWNELFLRGFDIDIRTSLAEEMR
                     KRGIDLRFKADIVRIDKDDGTLTATLTNGSVIEADQILYATGRTSKTPNLGLANAGIG
                     LKKNGAVIVNEYSQSTVENIYAIGDCTDRMMLTPVAIAEGMAVAQTLFNDNPTKPNYL
                     NVPTAVFSTPSCGTVGLTEDQARASNLEIDVYRSRFRPLKHTLTGRDERTMMKLVVDR
                     DTDQVLGCHMVGADAGEIIQGLAIALNCGATKAQFDTTIGIHPTAAEEFVTMRTKILD
                     "
     gene            5963..6727
                     /locus_tag="VFY96_14465"
     CDS             5963..6727
                     /locus_tag="VFY96_14465"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX5607725.1"
                     /translation="MDRETFREQLLAVMEKKVHWAWPMFTSGQVRKDRLHFHFEQEYE
                     TYVRDFPILVGRAYVRCPIPEIRRSLAENLYEEETGGLVAGSPHPLLFLEYPRGLGMD
                     LKRFEEVELLPAAKRYRAFLDDATEHFGWDIAAAIVTIFVEGSSDERAALELKEQKPP
                     PPLEEHPLVKYYGLPIARLALTKAHRQVEGSHRAAAWGSILNHVLPMRRGAVVRTMNE
                     ALDLWLAYRDGVAESCGLMRPNAGAEAAVDSLAEVA"
     gene            complement(6734..>7387)
                     /locus_tag="VFY96_14470"
     CDS             complement(6734..>7387)
                     /locus_tag="VFY96_14470"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX5607726.1"
                     /translation="TVMEHNNVDVAFAYEQCCGMPRWHSGDMDGALKAAEETVSRLIG
                     YVAEGRTVVATNPTCSQMIRAEYPRMLGTGEAKTVASQTMDPMEFLARLAAQGKLKRD
                     FKTGAGEISYHIPCHLRAQNVGYKTRDVLEMLPETKVRVVEECSGHDGTWAMKKENFE
                     QSLKWGNRAFSAMAEGNPRATCSDCPLAAIQIEQGNGRRPLNPMQILARSYRGEPIV"
BASE COUNT         1584 a         2022 c         2128 g         1653 t
ORIGIN      
        1 ttgtgggtcc atgaggggcg cgcggaaccg gttaagatcg cgataccgag cacgacacag
       61 gcggtgctgc cgttcaccat cgcgcgcgac aagggctatt atcgcgccga gggattggat
      121 gtcgagctga ttttggtcag cgcgccgatt gccagccgtg cgcttctgag cggcgacgtg
      181 gccgtcgcca cggtcggcgg cgctggactg ccacccgtct tacgcggctc gccgtttaaa
      241 tttttattta ccacctacaa ccgggcgatg ttctggctat tcgccaagcc cgagatccgc
      301 gatgtgcgag gactaaaagg aaagcgcatc ggcgtctccg ggatcggcag cggcccggat
      361 tctttgctgc gcgagatctt gcgacaaaat ggtcttgatg cgaatcgcga cgttgcggtt
      421 cttagccttg gagtgatgcc gactattttc gccggattgc aggcgggaac cgtggacgcc
      481 gctatgcttt cgcccccggt gacgttcaaa gcggaggact ccggatttcg cgagctcgtt
      541 tcttttccga agcaggatct cgtcgagctt caaggaagcg ttcttgtcag agaggcatct
      601 ttgcagtcgg atgggccgca aatggaacgt ttcctgcgcg gcacctacaa gggatttctc
      661 cacatcaaag aaaatcgagc cggcacgatt cccttcctcg cgcgctactt gcaactgaac
      721 caggaactcg cgacgcatgc ctacgatcag gttgtcaggc ccgcgatgac tcaggatggc
      781 acgctaagct cggagatgca ggccaaagca gtggaacatg tactcaagcg gctcgatctc
      841 aaagaagcgc cgccgctggc gaagatcttc gatttctccc tgacccgcaa agttgcgaac
      901 gagttgcaag caaagaactg gcgaccgtga ggcggcgcgt tcgctcgtgc tttaacagga
      961 aaatttcatg gcattgaagc tcaacatgac ctgcgcgctc tatgatcgca gccagccgct
     1021 gatcgaccgc cgggtaaagc cgcgcgatat cgatctcgag attcacgtga attccgatga
     1081 ccggagacgg cagcacgagg gccgcaatgg taaattcgat atcgtggagt tctttacggg
     1141 gatctacatg gcggatctcg aacaccgcag cttgggattt accgccattc ccatctttgt
     1201 caaacgcatg tttcgccatt cctacattta cgtgaataag cgcgtcggaa ttcgcgagcc
     1261 gaaagatctc aacggcaggc gggtaggttt gcaaacgtgg tttacgacca ccgcgctgtg
     1321 ggcccgcggc attctggaag acgaatacgg cgtcgatctg cgctcgatca cctggctcgc
     1381 caattggcac gagacggtag gtaattggca gcagccgtcc tcgctcaagc tcgagatcgc
     1441 gccgccaggc gtcaagcttc atgatttgct cgtcgccgga aaaattgacg cgggcatcac
     1501 gacggaaagt tgggcaccgt tcggccatcc cgacgtcgat tttttactgc cgaattatgc
     1561 cgaagaggaa cggcgttact acaaaaagac cggctttttt ccaattcagc acacgctgct
     1621 gatcaggaac gccgtattgg aacaacatcc gtgggtagcg atgagcacgt ttgacgcctg
     1681 gcaggaggcc aagcaggagt gctatcgctg gctcgatcgg cagcgcgtgc acatgaccgg
     1741 cttgtggtat cgcgccctat gggaagagga gcgcgcgctt gccggcgccg atccgtatgt
     1801 ttggggcttt aaaaggagcc gtccggaaat cgataaaatg ctcgaatacg cgcatcgtca
     1861 aggcttagta tcgggtaaat ttcgacccga ggacatgttt cacgggagta ccctggagac
     1921 gtaaaaatac tgtttctata aaattgccgt ttgtagggcg accggcggtc gccctaacag
     1981 gcatcgacga gggcacgatg gatctttccg aagttgttca gaccggccca ggaacgctgg
     2041 ccggacgttt tctgcgcatg ttttggcagc cggtttgttg ttcttatgag cttgctgccg
     2101 gccgtccgct cccgatccgt atcttgggcg aggatttcac gctctaccga ggcgagagcg
     2161 gtgaccctca tcttgtcggg ccgcgctgcg cgcaccgagc aacgcagctt tcggtcggat
     2221 ggatcgaagg ggagtgcatt cgctgcttct atcacggctg gaagtacgac ggcagcggcc
     2281 agtgcgtcga gcagcccgcg gaaggagaat cgttcgccga caagatccgc attcccggat
     2341 atccagtcaa agaatacatg gggctggtct tcgcctacct cggggaaggt gagccgccgc
     2401 cgctgccgcg ctatccccgt tttgagacct ccgaggtttc tctcgacgtg gccggcatcc
     2461 ggcgcatctg caattacttc aacaatatcg acaactcgct cgacagcgtc cacgttcgct
     2521 tcgtgcacca gcgccatcga gactccgtgg acgatcatgt ggtgctcggc gatccgatca
     2581 tctctgtcga cgagacccag tggggagtga ggcgatacgt caaatatccc aacggcaagg
     2641 acgttacttt tttcttcggc atgcccaaca tcaatttcat caacgggcag gtggtcgatc
     2701 ctgtgatcaa gcgcgccgac gtcatggtct ttaaagttcc tgtggacgat gaaagtcaca
     2761 tccatttcga agtgcgggca attcctctca ccggggagag aggacgcgca tggatcgagc
     2821 agcggcgcag gctgcgcgag caagcggaac aggaccggcc ggatttggtc cgggcgatac
     2881 tatcggggaa gcgccgcctc gacgatatcg accccaatcg ggtcgatttc gtgatgctgg
     2941 aggacgaagt cgcgcaggca gggcaaggcg caatcgcggt ccgcgcgaac gaacacctgg
     3001 gccgaagcga ccggggtgtt tttcttttac ggaaaatttg ggagcgagag ctgcgaaact
     3061 tagccgaagg caggccgctg aagcagtgga cttaccggcc ggatatggta ccgacatatc
     3121 cgaagactta ggaaatgggc aattgctcgc cctcagagta gcttcagacg agaattcgtg
     3181 ggcttgcagc caagcctatg cggcttcctc cggcgccccg cccgacttcg ccagatcttc
     3241 taccttgatt ttttcgacgt tcattttctc agccgctttg gtcatcctct cgatgacgtc
     3301 atcgaagccg aggggtgtgg cgtccttcgg gcggaaacgg atcggattga tgcgcgcgac
     3361 cacgaagctt ttcaagtaag ggctggtgag ccctttcgac tttaaagcct cgactttgct
     3421 gaccacggtt ttgtcgagat ccatcaatgc ttttgcgcga tcacgacgaa tatgcagcgc
     3481 ggccgcaaga ggctttttga tgaattggtc agaacgcctg aggatcgggt gataagcccc
     3541 gccgctgaaa cggccatttt caagatagca gagacccagt gtgatgagcg cggcctcctc
     3601 gaattccaat ccataggttt cttccgtggc cccgtccaaa gctgcgagct cttcatacat
     3661 tttgatcact tccaacgcgc gttctcgcag gttatgcgcc ttctcggtgt tgagggcgag
     3721 aatcttataa gcggcgctcg tctccggtac gacgagacca atgatagact tggcaccaag
     3781 ggtcttcatg gcagagaggc gatgattgcc attgggcgtc cagtagcgag cttcacttcc
     3841 cttgcctgct ctccgcacca cgataatcgg atcgagaaaa cggccaagct tcgcaatcac
     3901 gccttcgagc tttcgcgcat gcgcatcgga caagttgcgc tgatacggag tcggctcgac
     3961 ctgttcgatg ggcaatgctg cgagtaacaa ccagtgaccg ccgaacggct cgcgataact
     4021 cgcgagcact ttccccccat cgtcagcgac agctcgttgc agctcttcaa gatcgcgagg
     4081 ggcatctgtg gagagcaaat cgattggagc aagcccaatc gatttacctg cgagctttct
     4141 gcgcttgcgc gccgcgccgc tttttttctt ggtgcttttc tccgccatac accgtacccg
     4201 ccgctcctga ttatcatggg attcggggtt gttccaagcg caattggctc tttcaactcg
     4261 gccggtatga acgtggtggc aagactttca tgcccatgga tgatactttt attagtccgt
     4321 ctgacatcaa cgagagggcg ttatggctgg atacgattat gatttattta ccatcggtgc
     4381 gggctcaggg ggtgtccgag cgagtcgcat gtctgccgcg tttggcgcga aagtcgctgt
     4441 agcagaggaa cgttacctcg gcggcacatg cgtgaacgtc gggtgtattc ccaaaaagct
     4501 tctcgtctat gcctcgcatt acggcgagga tctgcatgac gcggcgggat acggctggag
     4561 cgctgcaggc caattcaatt ggtctacgtt gattgagaac aagaacaagg aaatccaacg
     4621 gctgaacgag gtttatcgaa agcttctcaa tgatgccggg gtaacgatat atgaagctca
     4681 tgccgaaatt gtcgatccac acacggtttc ggtggatggc aaggagatca cggcgcaata
     4741 cattcttgtc gcggtgggca gttggccggt ggtgccaaaa tttccgggct cagaatacgc
     4801 gatcacctcc aacgaggcgt tttatttgcc ggcgttgccg gagcgtgttg tcgtcgttgg
     4861 cgggggctat atcggtgtag aattcgccgg catctttcac ggtttggggg cgaagacgac
     4921 gcagctttac tggaacgagc tttttttacg tggcttcgac atcgatattc ggacaagtct
     4981 cgccgaggag atgcgcaagc gcggcatcga tcttcgcttc aaagcggata tcgtaagaat
     5041 tgacaaggat gacgggacac tcacggcgac tttgaccaac ggcagtgtta tcgaggctga
     5101 tcagattctt tatgctaccg gcagaacatc aaagaccccg aacctcggat tggcaaatgc
     5161 cggaatcggc ctcaaaaaaa acggcgcggt tatcgtcaat gaatattcgc aatcgacggt
     5221 ggaaaatatt tatgccatcg gcgactgcac agaccgcatg atgttaacgc ctgtggcgat
     5281 tgccgaaggc atggcggtgg cccaaacgct cttcaatgac aatccaacca agcctaatta
     5341 cctaaacgtc cccacagccg ttttcagcac gccgagttgc gggaccgtag ggcttaccga
     5401 ggaccaggca cgggccagca atctcgagat cgatgtttac cggagccgct tcaggcctct
     5461 caagcacacg ctcaccggcc gtgatgaacg gaccatgatg aaactcgtag tcgaccgtga
     5521 tacagatcaa gtgctcggct gccacatggt aggcgctgat gccggggaga tcattcaagg
     5581 cttggcgatt gcgctcaact gcggcgcgac taaagctcag ttcgatacga cgatcggcat
     5641 tcatcctacc gccgccgagg agttcgtgac catgcgaacc aagatcctcg attgagattc
     5701 agttctgcgt gcccgagcac gggagccaat cggtgaccac catggatcag tcgaaaaacg
     5761 attgtgcagg taagagagat gtccgcgagc accttttgag aacagttgcg atcggcagga
     5821 tagcccgatt ttccctacaa attgcagtcg taaccattct gccagtgggc tgttgacgtt
     5881 ccgtaccgag gaacatcaag cgtttatgct ttggcatttt cgttgcaccg gcgagcagcg
     5941 aaatccagct tgcgaggcaa caatggatcg agaaactttc cgcgaacaac tactcgccgt
     6001 aatggaaaag aaggtccatt gggcctggcc gatgtttacc tcaggtcagg taagaaaaga
     6061 tcgactgcat tttcatttcg agcaagagta cgaaacctac gtgcgcgatt ttcccatcct
     6121 ggtcggccga gcttatgttc gctgcccaat tccggagatt cgtagaagcc tggcagagaa
     6181 cttgtacgaa gaggaaactg gaggcctggt cgccggcagc ccgcatcctt tgctgtttct
     6241 cgagtatcct cgcggtctcg gaatggattt gaagcggttt gaagaggtcg agcttttacc
     6301 tgccgcaaag cgctaccggg cttttctcga cgatgctact gagcacttcg gctgggacat
     6361 tgccgcggcc attgtaacca tttttgttga aggatcttcc gatgaacgcg cagccttgga
     6421 gctcaaggag caaaagcccc cgccaccctt ggaggaacac cctctggtga agtattacgg
     6481 tttgcctatc gctcgattgg cgctgacgaa agcgcaccga caggtggagg gatcgcatcg
     6541 tgccgcggca tggggctcga ttctcaacca cgtgcttccg atgcgccgcg gggcagtggt
     6601 tcgaaccatg aacgaagccc tcgatctctg gcttgcttat cgtgacggcg ttgcggaaag
     6661 ctgcggtttg atgcgcccga acgctggcgc ggaagccgct gttgattcac tagccgaagt
     6721 ggcttgaata ggtttaaacg atcggctcgc cgcggtagct cctggcaagg atctgcattg
     6781 gattgagcgg ccggcggcca ttaccctgct cgatttggat cgcggctaac ggacaatcgg
     6841 aacaagtcgc tctgggattc ccttcggcca ttgcgctaaa agcccgatta ccccacttta
     6901 gcgactgctc aaaattttct tttttcatcg cccaagtccc gtcatgaccc gagcattcct
     6961 cgacaacccg taccttggtt tccggcaaca tctccaaaac gtcgcgggtt ttatagccga
     7021 cattttgcgc gcgcagatga caaggaatgt ggtaactgat ttcaccagcg ccagtcttaa
     7081 agtctctttt caattttcct tgtgccgcaa ggcgcgccag gaattccatc gggtccatgg
     7141 tttggctcgc gaccgtcttg gcttcgccgg tgccgagcat gcgcgggtat tcggcgcgga
     7201 tcatctgtga gcaggtcgga ttggtggcga ccacagtgcg gccttcagcg acgtacccaa
     7261 tcagccgtga aacggtttcc tcggcggcct tgagcgcgcc gtccatatcg ccgctatgcc
     7321 aacgcggcat cccgcagcat tgctcgtatg caaacgctac gtcgacattg ttgtgctcca
     7381 ttacggt
//
LOCUS       DASULZ010000698         3307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_789762, whole genome shotgun sequence.
ACCESSION   DASULZ010000698 DASULZ010000000
VERSION     DASULZ010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789762"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1895)
                     /locus_tag="VFY96_14475"
     CDS             complement(<1..1895)
                     /locus_tag="VFY96_14475"
                     /inference="COORDINATES: protein motif:HMM:NF012676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX5607727.1"
                     /translation="MPAKISRLDARRDHEALEWAKELRETETWFHSERFGHITRLHTP
                     FEVVALRGSLQEDYTIARESARKMHDYLQRLFVARKQEITYGPFSPTGAVRAVMEGIK
                     VLYLGGWATSAKGSESEDPGADLANYALDRVPKEGGAWVRALLHQDEVQRSNRIRMTS
                     LQRKKTPAIEFNPPMIIPDGDTGHGGEHHIRNLVKKFVENKIGAIHIEDQRGGCKVCG
                     HQGQKVLVSTAEIIARLNTARLQFDAMKVPGVIVARTDAHDATAIDTVDDERDHPFVY
                     GATHRDIVPFKNVNLAVIRKFYEEGFREINGHLLHRISEEAYKTAGEWLKKEHLTKRI
                     TEGIEKINKEAAALKRLRQQARHQRTGQRKFREELATLEIMIKKVTEETLDSVLATVR
                     QVWAEKAGLKTYADAVAEVMETRQQNKTKLPMPIDRWRKFAKGVSHEEARARAKSMDI
                     DIFWDWDLPRTSEGFYPITGGREMAIARGLAMAPFADLVWRETAKPDLADDKDWAEAI
                     HAVFPHTMLAYNLSPSWNWDAWGFTDGQIRSYAYELGKMGYVYNFITYGGHQTEALMN
                     GRLARALREEGVLGFVRLIQRALRLANDPAQYPQSFGGGGWADRFRRAARGPSLTTTS
                     MGGKSTET"
     gene            complement(2326..2802)
                     /locus_tag="VFY96_14480"
     CDS             complement(2326..2802)
                     /locus_tag="VFY96_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX5607728.1"
                     /translation="MKPYALLRSLLLGNRSWEQFSILLARVSLGMFFAISGGNKLFVA
                     SHTRRMYETLAGAGIPFPHFMTYFVSSVEFISGCLLVIGLLSSLCCAALIIQMIVAIT
                     TVQLATISKGLSFLDWLDDFLYLPETMYIIILVWLICSGPGRVSVDDWIARRGKMI"
BASE COUNT          731 a          939 c          852 g          785 t
ORIGIN      
        1 gtctcggttg attttccgcc catcgaagtg gttgtcagcg acggccccct agcggcgcgc
       61 cgaaaccggt ccgcccaccc cccaccgcca aacgactgag gatactgtgc tggatcattg
      121 gccaagcgga gcgcgcgctg aatcaatctg acaaaaccca gcacgccttc ttctcgtagc
      181 gcccgcgcca agcggccgtt catcagggcc tcggtttggt ggccaccgta agtgataaag
      241 ttataaacgt agcccatctt gcccaactcg taggcatagc ttcggatctg gccatcggta
      301 aatccccagg catcccagtt ccaagacggc gagagattgt aggccagcat cgtgtgcggg
      361 aacaccgcat gaattgcttc ggcccagtct ttatcgtctg ccaagtccgg tttagcggtt
      421 tcacgccata ccaggtcggc gaaaggcgcc attgccaacc cgcgagcaat cgccatttcc
      481 cgcccgccgg taatcggata gaaaccttcc gaggttctcg gcaaatccca atcccaaaaa
      541 atatcgatgt ccatggactt tgcccgcgct cgcgcttcct catgcgatac gcccttggca
      601 aatttcctcc accgatcgat cggcatcggt agtttcgtct tgttttgctg ccgcgtctcc
      661 ataacttcgg cgaccgcatc ggcatacgtt ttgagcccgg ctttttcggc ccaaacctgg
      721 cggaccgtcg cgagcacgct atccagcgtc tcttcggtga cttttttgat catgatctca
      781 agggtcgcca gttcttcgcg aaatttgcgt tgaccggttc gttggtggcg agcctgctgg
      841 cgtagccgtt tcaaagctgc cgcttccttg ttgatcttct cgatcccttc ggtaatccgc
      901 ttggttaagt gttctttttt cagccattcg cccgcggtct tatacgcctc ttccgagatc
      961 ctgtgcagca gatgtccatt gatttccctg aatccctcct catagaattt tctaatcact
     1021 gcgagattga cgttcttgaa cggcacaatg tcacgatggg tggccccata aacgaacgga
     1081 tggtcccgct cgtcatccac ggtatcgatc gccgtcgcat cgtgagcatc ggtccgggca
     1141 acaatcaccc ccggcacttt catggcgtca aactgtagac gcgcggtatt cagacgcgcg
     1201 attatttcgg cggtagaaac aagaaccttt tgtccttgat gtccacagac cttgcatcct
     1261 ccgcgttggt cttcgatatg gattgcgcca attttgttct cgacaaattt cttgaccaga
     1321 ttacgaatgt gatgctcgcc cccgtgtccc gtatccccat cgggaatgat catgggcggg
     1381 ttaaattcga tggccggcgt tttcttccgt tgtaacgatg tcatcctgat ccggttggac
     1441 cgttgcacct catcttgatg caacaaggcc cgcacccacg cacccccttc cttgggcacg
     1501 cggtccaaag catagttggc gaggtctgcg cccgggtcct cggattccga tcccttggcg
     1561 gaagtggccc agcccccgag atagagaact ttgatgcctt ccatcaccgc tcgcaccgct
     1621 cccgtcggcg agaaggggcc ataggtgatc tcctgttttc ttgcgacgaa aagccgctgc
     1681 agatagtcgt gcatcttgcg agccgattct ctggcgatcg tgtaatcctc ctgcaggctg
     1741 ccgcggagcg ccacgacttc gaagggggtg tgcagacggg taatgtgacc gaacctctcg
     1801 ctgtgaaacc aggtttccgt ctcgcgtaac tcttttgccc attccagggc ttcatgatcg
     1861 cgccgtgcgt ctagtcggct gatttttgca ggcatgggga atgcctccta aaatgaggat
     1921 gttgagatat cagggacctt agacgatcac tttcaactgc gaattacaag taaagccttt
     1981 aggttcaccg tcgacgaccc agacgcagta tactgttgac tcccaacatc ggcgccattg
     2041 cagcttgcca gaccgctttt cgaacgatct ctcacggttc agtcggtcac caaccgcgag
     2101 cacgaacacg gtattttgtc tttcaggcac catgctctgc cgctcacgag gtcgaaaaaa
     2161 tggttgttta aggagtgtga gtaagcgcca gtctaaagcg ccggccgacg taataagtga
     2221 tgtcgcgcaa gaccgatgtc ttgctctgta agacaacttt gcgggagaga tagaaattgt
     2281 caagattggc tagagtagaa attctgaccc tgtcgtttcc cctcttcaaa tcattttccc
     2341 gcgacgcgca atccagtcat caacgctgac tcttcccggt ccggagcaga ttaaccagac
     2401 caagatgatg atatacatgg tttcaggcag atagaggaaa tcgtcgagcc agtcgagaaa
     2461 cgagagccct ttcgaaatcg tcgcaagctg caccgtcgtg atcgcgacga tcatctggat
     2521 gataagagca gcgcagcaca ggctcgaaag caacccgatg acgagcaggc agccgctgat
     2581 gaactctacc gaggagacga aataagtcat gaaatgcgga aaaggaattc cggcgccggc
     2641 gagggtttca tacatccggc gagtgtggct ggcaacgaag agcttgtttc ctcccgaaat
     2701 ggcgaaaaac attccgagcg aaacgcgggc gagtaggatg gaaaattgct cccacgaacg
     2761 gtttccgagc agcagtgacc ggagcaatgc ataaggcttc atggcgttag tttctctgca
     2821 agtttcctat gaaaagcgct ccgtcgaacg tccgctcgcc tcacgcgcac ggtttcacgt
     2881 cacttgtaaa tgatagcgcc cttcacagca ctatctagtc cagcccacgc atggcgaata
     2941 aatgctctgc atccaaacca gaaccagata acggcagaga tgagatatgc attccacgat
     3001 agcatcgaaa ccccagcggc ttgatgacta accatagggt caggacctgc agaatggtta
     3061 ttggcatagt gaacaaggac atacctccag cagccgacga aacagtgtga cgattctgca
     3121 ggtcctgacc ctatggttca gtcatcaagc cgctggggtt tcgatgctat cgtggaatgc
     3181 atatctcatc tctgccgtta tctggttctg gtttgggata cagagcattt attcgccatg
     3241 cgtgggctgg actagatagt gctgtgaagg gcgctatcat ttacaagtga cgtgaaaccg
     3301 tgcgcgt
//
LOCUS       DASULZ010000699         2792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_383518, whole genome shotgun sequence.
ACCESSION   DASULZ010000699 DASULZ010000000
VERSION     DASULZ010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383518"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..756
                     /locus_tag="VFY96_14485"
     CDS             148..756
                     /locus_tag="VFY96_14485"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5607729.1"
                     /translation="MASTSNPSHDHDHHQWDSREYVFKWAAGQDPKEKDRETAFRVLA
                     DTIAFDRDEPIRILDLGAGYGALTQFLLNRFPHATAVCQDGSQEMAKLGAERMKKFSA
                     RFEYALCDFAKKGWQHHLQGTFEAVVSSIAIHNLREPKLIERIYHETYPLVKSGGCFL
                     NFDRPRPPWEDQMAWLRDAGFTQVNIFWRGENRAVFGGFKDK"
     gene            760..1779
                     /locus_tag="VFY96_14490"
     CDS             760..1779
                     /locus_tag="VFY96_14490"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEX5607730.1"
                     /translation="MRTRWILFLVFFWLLAIGACREKPSSRVPADPAKLRIVTSVAPI
                     TSIVENVVGSKAEVAGIIPEGINSHTFEPIPSDSKLLSSADLIIFNGLDLETPTQKLA
                     LANLKQGAVIYTLGDKTIKRDEYIFDFSFPKEQGHPNPHVWLNPQYAIRYATLVRDEV
                     VRLDPKNRDAYEQNVAQFIKKAEALDQAIQAATQTIPRNNRRLLTYHDSWPYFARRYG
                     FQVIGAVQPSDFADPSPREVMRLIDQIRREKIPAVFGSEVFPSPVLEQIAREAKSRYI
                     DKLRDDELPGKPGEPRHSYIGMMLDNMTIMFEALGGSSESLKNVDPAPVAGAHADYRG
                     KTKPS"
     gene            1799..2662
                     /locus_tag="VFY96_14495"
     CDS             1799..2662
                     /locus_tag="VFY96_14495"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEX5607731.1"
                     /translation="MANPHDHRPGDPHSLHPSDAEERLWHKATGVPLVDLAHVSSRYG
                     TTVALENVSLKIWPGQFMAIVGPNGGGKTTLLRTILGMVPAAAGKVLLRGHPLTRSSL
                     DRIGYVPQLETIDWNFPITVEEVIAMGFYVKNRWFGGIGEKEQRKLENIMERLNLAGL
                     GKRHIRELSGGQQQAVFIGRALLGDPDLILLDEPASGLDIRSRDDVIHFLHEVNHQGV
                     AIVLTAHDLNWVAAHLPWAVCINHRMIAEGRPNEIFNAKVLREAYSGELVVFHHDGMV
                     MIGERPHAPHT"
BASE COUNT          642 a          812 c          759 g          579 t
ORIGIN      
        1 ggcggcgccg cattcgatga agaggctttg aaagctgtcc gacaatcccg tttcgagccc
       61 gcacaaaggg acggacagaa cgtcgcggca gactttaatt atgtttatcg tttcagactg
      121 caaagatgaa acgagaggag aattcacatg gcaagcacat ccaatccttc ccacgaccac
      181 gatcatcacc agtgggattc gagggaatat gtcttcaaat gggccgcagg tcaggaccct
      241 aaagaaaaag accgtgagac agcatttcgg gtgcttgctg atacgatcgc tttcgatcgc
      301 gatgagccga ttcgcatttt ggatctcggc gccggttacg gcgcccttac ccaatttctg
      361 ctaaatcgat ttcctcatgc cacggccgtg tgccaggacg gttctcagga aatggcgaag
      421 ctcggcgctg agcgtatgaa aaaattctcc gctcgattcg agtatgccct ctgcgatttc
      481 gccaaaaaag gttggcagca tcatctccaa gggaccttcg aagcggtggt ttcgtctatc
      541 gccattcaca acctccgcga gccaaagctc atcgaaagga tctatcacga gacttatccg
      601 ctggtgaaat caggcggttg tttcctcaac ttcgatcgcc ctcgtccgcc ctgggaagat
      661 cagatggcat ggctgcgcga tgccggtttc acacaggtga acatcttctg gcgcggagaa
      721 aatcgcgcgg tctttggcgg gttcaaagac aaataagcga tgcggacccg gtggatactt
      781 tttctggttt tcttctggct gctcgcgatc ggggcttgcc gcgaaaagcc gtcgagccgg
      841 gttcccgccg atccggcaaa gctgcgaatc gtcaccagcg ttgcgccgat cacaagcatc
      901 gtggaaaacg tggtggggag taaagccgag gttgccggga tcattcctga aggcatcaac
      961 tctcacacgt tcgagccgat cccctcggac agcaagctcc tgagcagcgc ggacctgatc
     1021 attttcaacg gcctcgatct ggaaactccg acccagaaac tcgctctggc aaatttgaag
     1081 caaggcgcgg tgatctatac cctcggcgac aaaaccatca agagagacga atacattttc
     1141 gacttcagct ttcccaagga acagggtcat cccaatccgc acgtgtggtt gaatccgcag
     1201 tacgcgatcc gctacgcgac tctcgtccgt gacgaagtgg tccggctcga tccgaaaaac
     1261 cgggatgcct acgagcagaa cgtggcgcaa tttataaaga aggccgaagc gctcgatcag
     1321 gcgatccagg cggcaaccca gacaatcccg cgcaacaacc gccggctgct gacctatcac
     1381 gattcctggc cttactttgc gcgccgttac ggctttcagg tgatcggtgc ggtgcagcct
     1441 tccgatttcg ccgatcccag cccaagggaa gtcatgcggc tcatcgatca gattcgcagg
     1501 gaaaaaattc ctgcagtgtt tggctccgag gtattcccaa gcccggtgct ggagcagatc
     1561 gcgcgtgaag cgaagagccg ctatatagac aagctgcgag acgatgaact ccccggcaag
     1621 cctggcgagc cgcgtcattc ttacatcggc atgatgcttg ataatatgac gattatgttc
     1681 gaagcgctcg gcggctccag cgagtcgctc aaaaacgttg atccggcgcc cgtcgccggt
     1741 gcgcacgccg attatcgagg taaaaccaag ccgtcataat tctgatagca actgccccat
     1801 ggccaatccg cacgatcatc gacccggcga cccgcacagc ctgcaccctt cggatgccga
     1861 agagcgcctc tggcacaagg ccaccggcgt accgctcgtc gacctggcgc acgtcagcag
     1921 ccgatatgga acgacggtag cgctggaaaa tgtctcgcta aaaatctggc caggccaatt
     1981 catggcaatc gtcggcccca acggcggcgg caaaaccacg ctgctgcgca cgattctcgg
     2041 catggtgccg gccgcggcgg gaaaagtcct gctccggggc catccactca cgcgctcgtc
     2101 tctcgatcgc atcggctacg tgccgcagct cgaaaccata gattggaatt tcccgatcac
     2161 tgtcgaggaa gtgatcgcca tggggtttta tgtcaagaac cgctggttcg gcggcatcgg
     2221 cgagaaggag caaagaaaac tcgaaaacat aatggagcgg ctgaatctgg ccggactagg
     2281 caaacggcat attcgggagc tctcgggtgg tcagcaacag gcggtcttca tcggccgtgc
     2341 gctgctcggc gatcctgatc tcattctgct cgacgagccg gcctccggtc tcgacatccg
     2401 ctcacgcgat gacgtcattc acttcctcca cgaggtcaat caccagggcg tcgcgatcgt
     2461 cctgaccgcg catgatttga actgggtggc cgcgcatctg ccctgggcag tctgcatcaa
     2521 tcatcgcatg atcgccgagg gccggcccaa tgagatcttc aacgctaaag ttctcagaga
     2581 agcctacagc ggcgagctcg tcgtattcca tcatgacggc atggtgatga tcggtgagcg
     2641 cccgcacgcg ccgcatactt agataaaagc tgttcaaacc gttccagccg ttcaagtcgt
     2701 tcaaaacatc cgaagccggc ttgaaccgtt tgaacttttt gaacgatttg aactgtaatg
     2761 tctttactca ctccgtttga gtacgaattc tt
//
LOCUS       DASULZ010000700         3440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1500979, whole genome shotgun sequence.
ACCESSION   DASULZ010000700 DASULZ010000000
VERSION     DASULZ010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500979"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..588
                     /locus_tag="VFY96_14500"
     CDS             244..588
                     /locus_tag="VFY96_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lin0512 family protein"
                     /protein_id="HEX5607732.1"
                     /translation="MDLVRCVTEMGMGVDVHGLDYTNAAKRAVFDAIHHSSLGFPRLI
                     GKTADDMRVDVTIGVPKPEAVDKQAVLDTLPHGGGHVNVVHGGLQVLNEKGDDGWLIA
                     NAIIVVSIDDGK"
     gene            complement(624..929)
                     /locus_tag="VFY96_14505"
     CDS             complement(624..929)
                     /locus_tag="VFY96_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HEX5607733.1"
                     /translation="MEYYADRWPTDSLQARGNEYGTRAITNIAKVSPMPKAGGKWNTY
                     EITARGSHLTLVLNGIKTVDVQHSQFTEGPISLQFGNGEKGAPGGAIKWRKVQIRPF"
     gene            1030..2001
                     /locus_tag="VFY96_14510"
     CDS             1030..2001
                     /locus_tag="VFY96_14510"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607734.1"
                     /translation="MKAVIVLLISMLSFILSLAISSSGAEKLRIAYPTLGPGSTPSWV
                     TYETGFWKKSGLDVELILLSGGARMLPALISGSVDIILGSDTGVTTANLQGANLVRIG
                     VTMNSLGYALVTQPAIRSIHDLKDKVLGTGRGRDASYARLVKILSDNGLNPATDVKFL
                     PIGETPTGRLQAIKSGLIHGAVFTPPLDLLGSRDGLRILHKIDVPTLGGGINTTAAFL
                     QQNRKVLLTFLRAYMEGIRYTITHKTESLQVFFKYFRNPDSEALAYLYDDTAPRLQKD
                     LRPNPDSVRSILDQIALDDPRAKQLSVKDHWNLTLLDEIQKSGFVDR"
     gene            complement(2216..2743)
                     /locus_tag="VFY96_14515"
     CDS             complement(2216..2743)
                     /locus_tag="VFY96_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020161399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX5607735.1"
                     /translation="MMASRFPMTLLTCLLLTGYVSAQEKATTQEAEKIAEKVKQKAAR
                     SEKAEGRQNLVKDKVTVKQGEGGQPIIEQRGEASYYGPGFQGKKTATGEKFDQKERTA
                     AHPALPLGTKATVTNLENGKSVDVKINDRGPYVKGRDIDLSKSAAKDLGMTKDGVAPV
                     KIEAEVKAEKNKEKK"
     gene            3089..>3440
                     /locus_tag="VFY96_14520"
     CDS             3089..>3440
                     /locus_tag="VFY96_14520"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5607736.1"
                     /translation="MDSRINYLLLATALLGTFFSGTATRIVAISMPTVAQSLGTDLLG
                     VSWALLSYQLSNIGLSVVFGRISDLWGREKMFALGFVVFATSSLLCGMSQTVLQLILA
                     RFIQGIGGAMLQSSS"
BASE COUNT          730 a          897 c          956 g          857 t
ORIGIN      
        1 gaaaggataa aggcgaaagg aaagcattaa ggagaaccga agtaatttta tcctacctca
       61 tccttttaga acggttgcat cggaatgttt cgtgacttac tgtcgggaaa agtctatgcc
      121 ggcagagagt gcggtcgcta ccaaagagta gatccgggat tgcccggaac gacaaaaggc
      181 tgttaatgag tagctcgtct acatgtcaca gttggtcaac tgaattgtta gaggagatgc
      241 aacatggatc tggtacgttg cgtaacggaa atgggcatgg gcgtggatgt gcacggattg
      301 gattacacca atgctgcgaa gcgggctgtg ttcgatgcaa ttcatcacag cagcttggga
      361 tttccgcggc tgatcggcaa gacagccgac gatatgcgag tggacgtgac catcggtgtg
      421 cccaagcccg aagcggttga taagcaagcg gtgctcgaca cgttgcccca cggtggtggg
      481 cacgtcaacg tggtgcacgg cggtttgcag gtcctgaacg agaagggtga cgacggctgg
      541 ctcatcgcca atgcgattat cgtcgttagc atcgacgacg gtaaatagaa aacgagcagt
      601 ccagttgagt ccgatggcgc gagttaaaac ggcctgatct gcaccttgcg ccatttgatg
      661 gcgccaccag gggcgccttt ttcaccgttg ccaaactgaa gcgagatcgg accttccgtg
      721 aactgggaat gctggacatc gacagttttg atgccattga gaacgagcgt cagatgcgag
      781 cctctggcgg taatctcata agtattccat ttacctcccg ctttgggcat cggcgatacc
      841 ttggcgatat tcgtaatggc tcgggtgccg tactcgtttc cgcgtgcttg caggctatca
      901 gttggccagc gatcggcgta atattcgatc ggttgacgga cgctttttag tgatgaggtt
      961 ggcaccttcg atgcggcgac ttctgatccc gcgctacttc tttggaactg ttctcatgag
     1021 gtgaagaaca tgaaggcagt catcgtcctg ctgattagca tgctatcgtt tatcttgtct
     1081 ctcgcgatat cgtccagtgg tgccgagaaa ttgcgcatcg cttatcctac ccttgggccg
     1141 ggatcgactc cgtcctgggt gacctatgag acgggattct ggaaaaagag cgggctcgat
     1201 gttgaattga tcctgctgag cggcggcgcg cgcatgttgc cggcgctaat cagcggcagc
     1261 gtggatataa ttctcggctc ggatacgggc gtcaccacgg ccaatcttca gggcgccaat
     1321 ctggtgcgca tcggcgtgac gatgaattcg ctgggctacg cattggtgac gcaaccggcg
     1381 attcgttcga ttcacgattt aaaagacaag gtgctcggca cgggacgcgg acgggacgcc
     1441 agctatgcgc gtctggtgaa gattctcagc gacaacggac tcaatcccgc aaccgatgta
     1501 aagtttcttc cgatcggcga gactcccacg gggagactgc aggcgatcaa aagcggcctg
     1561 attcacggtg ctgtattcac gccgccgctc gatcttttgg gcagccgcga cggtttgcgg
     1621 atcttgcaca agatcgacgt gccgacgcta ggcggcggca tcaacaccac tgctgcgttc
     1681 ctgcagcaga atcgcaaagt gcttctgacg tttttgcgcg cctacatgga agggatccgc
     1741 tacacaatca cgcacaagac agagagcctg caagtgttct tcaaatactt ccgaaatccc
     1801 gacagcgaag cactcgccta cctctacgac gataccgcgc cgcggcttca aaaggacttg
     1861 cggccgaacc cggactcggt acgctcgatt ctcgaccaga tcgccctcga cgatccacgc
     1921 gcgaagcaac tcagcgtgaa agaccactgg aaccttacgc tgctggacga gattcaaaaa
     1981 tcgggttttg tcgacagata gtaccagcaa tgatggccgc ggtgagggta tgggtgattg
     2041 ggccgtagac gaactgggtt caaattttta ccgctcgagc ttcattggtg cgcccgcgta
     2101 acaccaagcg acttgcactc gattccagag gttcgaaccg ttccagtgtt tctgcccgag
     2161 ttgttcgaag gcttcatcgt cgacgagctc aggatgagcg gaaagatatg actcgctact
     2221 tcttctcttt gtttttctca gccttaacct ccgcctcgat tttcactggc gcgacgccgt
     2281 ctttggtcat acccaaatct ttcgcggcgc ttttcgaaag gtcgatatca cgtcccttaa
     2341 cgtaaggacc gcgatcgttg attttgacgt cgacggattt cccgttttcc aggtttgtaa
     2401 cggttgcctt tgttcccagt ggtagtgccg gatgagccgc agttcgctct ttttgatcga
     2461 acttctctcc agttgcagtc ttctttccct ggaaacccgg cccatagtat gaggcttccc
     2521 cgcgttgttc gatgattggc tgaccgccct cgccttgttt tacagtaact ttgtccttca
     2581 ccaagttttg cctgccttca gctttttcag aacgcgccgc cttttgcttt actttttctg
     2641 caatcttctc agcctcttga gtcgtggctt tctcttgggc tgacacgtat ccggtcagaa
     2701 ggaggcaggt tagcagtgtc atcgggaagc gggaagccat cattactctc tccacagatt
     2761 ggtttcattg gggcgtgcct ggcgggttgt actaatggca agcctcgtac caatcacgaa
     2821 tcgcctgaaa accgttaaca atcgtcaagc taggagcgtg tctcaaaacg agagactact
     2881 actttgaagg aattcgacgt gaaatgatca aaacttgtcg gcaattttcg tattgcacag
     2941 aggggtcata gccgccggtg ccttaaaaaa aggccatgtt tgtctgccgc aaattcttgc
     3001 atccggccct ggcaaatagg tgatttccgt ttgccgcgcg gcggcttcgg cgttatgaat
     3061 cgaaaaattt tctgcgtacc gcaacgaagt ggactcacgg atcaattatc tactgctggc
     3121 cacggcgctc ctcggaacat ttttctccgg cacggcgact cgcatcgtcg cgatctcgat
     3181 gccgacggtg gcgcagagct tgggaaccga tttgctcggt gtctcctggg cgcttttatc
     3241 gtatcagcta tcgaatatcg gattgtcggt agttttcggg agaatttccg atttgtgggg
     3301 gcgcgagaaa atgttcgccc tcggatttgt cgttttcgcg accagctcgc ttctttgcgg
     3361 catgtcgcaa accgtgctgc aactgatcct ggcgcgtttc attcagggca tcggcggcgc
     3421 catgctgcag tcgtcgagcc
//
LOCUS       DASULZ010000701         4582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1501018, whole genome shotgun sequence.
ACCESSION   DASULZ010000701 DASULZ010000000
VERSION     DASULZ010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501018"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="VFY96_14525"
     CDS             complement(<1..1095)
                     /locus_tag="VFY96_14525"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5607737.1"
                     /translation="MDPRTNWLLLINAALGNFLAGTSSRIFSVSLPTVANSLETTIVG
                     ISWAVIAYQISQISLSLIFGRIGDLYGRHTLFGIGLVVSSIGALLCGLSQNVGQLIAF
                     RLFQGVGASMTQSQGRALAMEALPKESAGRAQGFMTTAFHSGVLIGPSIGGLLIDYVH
                     WRAVFFFLIPIGIAGMVLTLINRWRGNAPQRPRRADAHGVIDYLGAVLLICATVALVA
                     CVDHRIMESLSGAVRAALVAVFLMLFSGFIFRERKARSPILDLSLFRIRMFTLSSLAL
                     LLVGVSQVMIGFVLPFYLQDVLHLAPSFIGLLFISAPVFTVTLSPFVGWAVDKVGPRL
                     PATVGILFLAAAGFFGTFLRTDSHWTMAVGI"
     gene            complement(1181..1579)
                     /locus_tag="VFY96_14530"
     CDS             complement(1181..1579)
                     /locus_tag="VFY96_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607738.1"
                     /translation="MSFDAKTILANLVEQERIKGHHSPEGRAIRTLARALSGWSAGTL
                     SAADVIILCEQSVEDWLKARLKRSPWSVESLPALLSAAVGQNLITPSEESPLQKIHQQ
                     RELAVQAGAISIEAVQAALEFCIQLIEKHW"
     gene            2018..2983
                     /locus_tag="VFY96_14535"
     CDS             2018..2983
                     /locus_tag="VFY96_14535"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX5607739.1"
                     /translation="MEPLPRGANFQRALMLWEIGLHIAGNPNTPYYGPRDMCIAVGSG
                     SNETFKPWLRRSTGSPILAHAVCRNELEAAMINPSGLLTQAYRGTGIFPEKLPVRIIA
                     VYPSLDHFVYLIHPRTGLKSLIDLKEKKYPLRLSIREDATHSTRVLVDQTLAAYGISV
                     KDIESWGGSLQLNGGPGDERRMKAIREGTIDAIFDEGLVLWLNEALESGMQPVTLEDF
                     AFKHLVDNLGWRKYTIKPGRYKNLKNEHTCIDYSGWPIYTRASLPDDDAYKICDAIMA
                     RKEQIYWEDSYTGVGQLGENTDATPRDVPLHPGAEKWYREHGFKV"
     gene            3425..4567
                     /locus_tag="VFY96_14540"
     CDS             3425..4567
                     /locus_tag="VFY96_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5607740.1"
                     /translation="MSDDRTKARDKSTIVHVLGLPGRNKLPTIEGRGDVSINFYKHDR
                     PAPLLFILPGVGANPFFGAAPYLASFFYRENFHVVILPSPMSWNFALSASRTGAPGYV
                     PEDARDLYTIMQRILATLKDRYDLRITKIDFMGLSLGALEGAFLSVIDADQKKIGIEK
                     YLLVNPPVDLAYAVKQLDEWTALGNKFGRDKSQEIVSTAIAIVDSFSGEKQDNPEIFD
                     RFIKKFAIFSKEELQFLVAQSLQSQLPELIYATQAIYDQKILSAPKNEMRKRLEEAKG
                     LTLTDYNEKIGLPLWRQQAAEPQADLASFIERGSLPYILDRLRDNPKVHIMHNADDFL
                     TNRTSIERLKEALGDRVMVYPYGGHLGNLWYPQNKKDVLRTFKTAP"
BASE COUNT         1149 a         1296 c         1170 g          967 t
ORIGIN      
        1 aataccgacg gccatcgtcc agtgagaatc tgtgcgcaag aaagtcccga aaaatcctgc
       61 cgcagccagg aacaggatac ccaccgtcgc cggcaaccgc ggccccacct tatcgactgc
      121 ccagccgacg aacggcgaaa gggttaccgt gaataccgga gcactgatga acaatagccc
      181 gatgaatgaa ggagccaaat gtaaaacgtc ttggaggtaa aaaggcaaga cgaagccgat
      241 catcacttgc gacacaccga ccagcagcaa cgccaggctg ctcagcgtaa acatgcgaat
      301 cctgaaaagt gacaggtcga gaatcgggct ccgcgccttg cgctctcgaa aaataaaccc
      361 gctgaacagc ataagaaata ccgccacaag cgcggctcgc acagcgccgc tcagcgactc
      421 cataatccga tgatcgacgc aggctacgag agcaaccgtg gcgcagatga gcagcacggc
      481 gcccagataa tcaatcacgc cgtgcgcgtc ggccctgcgc ggtctttgtg gcgcatttcc
      541 tctccaccgg ttgatcaacg tcagaaccat gcctgcgatg ccgatcggga ttaaaaagaa
      601 aaacaccgcg cgccaatgaa cgtagtcgat cagcagacca ccgatgctcg gtccgatcag
      661 cacgccggaa tgaaacgcag tggtcataaa gccttgagct cgtccggccg attccttggg
      721 caacgcctcc atcgcgagcg cgcggccttg cgattgcgtc atcgacgcgc cgacgccttg
      781 aaacaaccgg aacgcaatga gttggccgac attctgcgaa agaccacaga gcagcgcgcc
      841 tatggaggac accacgagtc ctatgccgaa aagcgtgtgg cggccgtaaa gatcgccgat
      901 ccggccgaag atcagagaga gactgatctg cgaaatttga taggcgatga ctgcccagga
      961 gatgccgacg atcgttgtct ccaaactatt cgcaaccgtc ggcagggaga ccgaaaaaat
     1021 tcgactcgaa gtgcccgcta agaaatttcc cagcgccgca ttgatgagca gcagccaatt
     1081 ggtgcggggg tccatgcgtt tggtggctca agggcggcaa gaaattgttc atgagccgag
     1141 cgcgcacgca cgcaacttgc cccggtccaa ctctctcgct ctaccagtgc ttttctatca
     1201 gctgaatgca aaactcaagc gccgcttgga ctgcttcgat ggaaatcgct cctgcttgaa
     1261 ccgcaagttc gcgctgctga tgaattttct gcagcggact ttcttctgac ggagtaatta
     1321 ggttttggcc gactgcagca gagagcagcg ctggcaacga ttccactgac catggtgagc
     1381 gtttcaaacg agccttcagc cagtcctcga ccgattgctc gcacaagata attacgtccg
     1441 ctgccgaaag cgtaccggcc gaccaaccgc ttagggctcg agccagcgtc cgtatcgccc
     1501 ggccttcagg cgaatgatgg cctttgattc tttcttgctc gaccaagttc gccagaatcg
     1561 ttttggcatc gaaggacatg ggcagacctt agataactta ccactaagtg ggcagaggtg
     1621 atcaagatac caattttcac cacgaagtga ttcatgagct tatgatggcc gatagagaat
     1681 aaaatctgcg cgacggagtc ccacgttgtc accccgaagg ttttaatcgg gggtccagtc
     1741 cgaatttctc tggattcccg ctaaaaccat gcgggaatga cggaaccgag atcgcaattt
     1801 taatccgacg cccttttcga aggcgacgct aattttgtag ggtgtgctct gcgcatccca
     1861 gcctcgatgc agtcttaatc tcttcacgcc tttgcgggag ataatctcaa tcagaccttt
     1921 gtgagcttcg tggtaaaaac gaaaatgagt cgcgctattg acacaaagac agcgccactc
     1981 ataatctgcg ataaatcgta tcaattgggg gtcactcatg gaaccactgc cgcgcggcgc
     2041 caattttcaa cgagccttaa tgctttggga aatcggcctg cacattgccg gtaatccgaa
     2101 tacgccctac tacggaccac gagatatgtg catcgctgtc ggcagcggct caaatgagac
     2161 cttcaagccg tggctgcgca ggtccaccgg gtcgccgatt ctggctcacg ccgtatgccg
     2221 caatgagctt gaagcagcga tgatcaatcc ttcgggactc ctgacccaag cctatcgcgg
     2281 caccggaatc tttccggaaa aactaccagt gcggattatc gctgtctatc cttctctcga
     2341 tcattttgtc tatctcattc atccgcgcac cggtctcaag tcactcattg atcttaaaga
     2401 aaagaaatat cccttgcgtc tttcgatccg tgaagacgca actcattcca cgcgcgtgct
     2461 cgtcgaccag acgctggctg cctacggcat tagcgtgaaa gacattgagt cgtggggcgg
     2521 cagcttgcaa ctgaacggcg gccccggcga cgagcggcgg atgaaggcga tccgcgaggg
     2581 cacgatcgat gcgatcttcg acgaagggtt ggtgctctgg ctcaatgaag ctcttgaaag
     2641 cggcatgcag ccggtcaccc tcgaagactt cgcctttaaa cacttggtgg ataatctcgg
     2701 ctggcgcaag tacacgatca agcctggtcg ctacaaaaac ctaaagaacg agcacacctg
     2761 catcgactac agcggctggc cgatctacac acgtgcgtcg ctacccgatg acgacgctta
     2821 taagatctgc gacgccatca tggcccgaaa agagcaaatc tactgggagg attcttacac
     2881 cggtgtcggt cagctcggcg aaaacaccga cgccacaccg cgcgatgtcc cgctgcatcc
     2941 gggcgcggaa aagtggtacc gcgagcatgg ttttaaagtc taaacccagg aagtttattc
     3001 aacgtcctgg catcgtctat tagtaaatta ttagcgaact gcctgtagtc accgcacaac
     3061 cagtcctcga tttggagcgt gccagttctg cgcctgcagt ccattgaaga acataaactg
     3121 acgtagcaca gggcgggtaa aaattttagc attaatagag cgatgcggtt gtcggtgtca
     3181 tggcctcgct ggggattctc cgttcggcat ggcttgtcat cgcaagcact catccacacg
     3241 cgcgaaaaaa caacacatca tgacgcatcg gctgacgcgg tcaacgcgac attttcccaa
     3301 gccccttcgt tttgcttttt tggcggtgat cgcgtcattg taccttgcct taaatcttgt
     3361 ccgagctgca gagtataact atccgtatcg cgatcccttt ttggcaacgg ctacgacggc
     3421 ccttttgagc gacgacagaa ccaaggcgcg ggacaagtcg accatcgtac acgtgctcgg
     3481 gctacccggc agaaacaagt tgccaaccat cgagggtcgc ggcgatgtaa gcataaactt
     3541 ctataaacac gaccgcccgg cgccacttct ttttattctt cccggtgtcg gtgctaatcc
     3601 gtttttcggc gcggctccct acttggccag cttcttctat cgggaaaatt ttcatgtggt
     3661 gattttgccc tctcccatga gctggaactt tgccctgtcg gcaagtcgta ccggcgcacc
     3721 cggttacgtt ccggaagatg cgcgtgatct ctatacgatc atgcaacgga tccttgcgac
     3781 tctgaaagat cggtatgatc tgcggatcac aaagatcgac ttcatgggtc tgagtctggg
     3841 cgctctcgaa ggcgcctttc tcagcgtaat cgacgccgac cagaaaaaga tcggcatcga
     3901 aaagtacctg ctcgtcaatc cgcccgtcga tctcgcttac gccgtaaaac agctcgacga
     3961 atggaccgcg ctgggaaata aattcggccg ggataaatct caggaaatcg tttcaacggc
     4021 gatcgctatt gtcgattcct tttctgggga gaagcaggat aatccggaga tcttcgaccg
     4081 attcataaaa aagttcgcga tcttctccaa agaagagctc caattcctcg tcgcccagag
     4141 tctccaaagc caacttcccg aattgattta tgccacgcag gccatatacg accaaaagat
     4201 tctcagcgcg cccaaaaatg aaatgaggaa acggctcgaa gaggctaaag gtcttacgtt
     4261 gaccgactat aacgaaaaaa tcggcctacc cctatggcgg cagcaagccg ctgaacccca
     4321 agccgactta gcaagcttta tcgaacgcgg ttccttgccg tatatccttg accgcctgcg
     4381 cgacaacccc aaagttcaca ttatgcacaa cgccgatgat ttcttgacca acagaacatc
     4441 catcgaaagg ctcaaagagg ccttgggcga tcgagtcatg gtttatccgt atggcggtca
     4501 tctgggaaac ctgtggtatc cgcaaaataa aaaagatgtt ctaaggacat tcaagaccgc
     4561 gccataggca cgaggcaaag tg
//
LOCUS       DASULZ010000702         4162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_181889, whole genome shotgun sequence.
ACCESSION   DASULZ010000702 DASULZ010000000
VERSION     DASULZ010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181889"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /locus_tag="VFY96_14545"
     CDS             <1..1139
                     /locus_tag="VFY96_14545"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5607741.1"
                     /translation="FIEHLLFKGTERRSAAQIAEEMDAVGGVINAFTAKEHTCYYAKV
                     LDENLPLAIDLLTDIFLNSSFDADEIERERSVILQEISQAEDTPDDYVHDLFNLDFFR
                     DHPIGRPICGREQTVNNFRREDIIEFFKSRYQPRRVIVSIAGNFQHAAVVDEMAARLG
                     SVQEQNHDPHPGVTGEAVPEMGSGIFPHPKSLEQVHLCLGVAAIPQSHPKRYIAYVLN
                     TLLGGGMSSRLFQEIREKRGKAYSVYSFSSAYKDVGYLGVYAGTSLDSTEEVVELILQ
                     ELKKMAAGDVSENEIARTQGQLVGSMILGLESTDSWMSHVARDEIYFGQAIGINEICQ
                     KIRAVSRDDVIELADMLFAKGAMTLTLLGDFDDKFQIKKLDLPA"
     gene            1173..1661
                     /gene="dut"
                     /locus_tag="VFY96_14550"
     CDS             1173..1661
                     /gene="dut"
                     /locus_tag="VFY96_14550"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX5607742.1"
                     /translation="MEQLRIEIKRIRPAANQLDLPAYMTEGAAGMDICADIDHDRTLA
                     PLERALIPTGVAIALPPGFEAQIRPRSGLALREGLTIINTPGTIDSDYRGEIQLIAIN
                     LGSEPIVIRRGQRIAQMIIQRVVRAAWVEVEELPASVRESGGFGHTDKTREELGVRSK
                     GR"
     gene            1695..2987
                     /gene="purB"
                     /locus_tag="VFY96_14555"
     CDS             1695..2987
                     /gene="purB"
                     /locus_tag="VFY96_14555"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX5607743.1"
                     /translation="MIPRYTRPEMGRIWSEENSFQKWLDVEILAAEGLARMGRVPKAA
                     ISRIRKKARLDVKRIRAIEAEVKHETIAFLSAVAETIGDDARYLHVGMTSSDVMDTAL
                     AIQFKEASAILEQDINDLMTVLRRQARKYKSTVMIGRTHGVHAEPITFGLKLAIWYQE
                     MERHLSRFRHAVEDICVGKISGAVGTFAQIPPEVEAYVCRKSGLKPAPVSNQIVQRDR
                     HAFYFSTLALIASSLEKFAIEIRHLQRTEVQEAEEPFTRGQKGSSAMPHKRNPILSEN
                     VSGLARLMRSYAHAAMENIPLWHERDISHSSVERVIAPDATIALDFMLHRMNYVLGSL
                     CVYPENMQRNLAKSGGAVFSEKVLLALVEKGLARDVAYRMVQAHALKVGKEGGDLKRE
                     LLADAEIRRYLPAQEIDNLWGIKHHLANIDVIFRRVFR"
     gene            3013..3261
                     /gene="purS"
                     /locus_tag="VFY96_14560"
     CDS             3013..3261
                     /gene="purS"
                     /locus_tag="VFY96_14560"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX5607744.1"
                     /translation="MRVRIFVSLKKGVLDPQGKAIERSLHVFGYNEVQDVRVGKFLEL
                     EVDATSREGAEARVREMCDKLLANPVIEDYRFEISPEL"
     gene            3429..4139
                     /gene="purQ"
                     /locus_tag="VFY96_14565"
     CDS             3429..4139
                     /gene="purQ"
                     /locus_tag="VFY96_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX5607745.1"
                     /translation="MRWGVITFPGSCDDHDVIDCLKTDLGQKVKVLWHKDELAGNFDC
                     LVLPGGFSYGDYLRCGAMARFSPIMRGVMKFAQSGGLVLGVCNGFQILCESGLLPGAL
                     VRNSGLRFVCQPVHLRVEETDTPFTSNLKKGQLLRMPVKHGEGCYYADAKTLESLRKN
                     RQILLRYVDAKGKPTASANPNGSVENIAGICNKTRNVFGLMPHPEDACSALLGSEDGA
                     KLFMSIAAACAVRVNPHA"
BASE COUNT          917 a         1100 c         1251 g          894 t
ORIGIN      
        1 atttcatcga acatttgctt ttcaaaggca ccgagcggcg tagcgctgcg caaatcgccg
       61 aagaaatgga cgcggtcggc ggcgtcatta acgcgtttac cgctaaagag catacttgct
      121 attacgccaa ggtgctcgac gaaaatctgc cgctggcgat cgatctcctg acggatatct
      181 tcttgaactc gagcttcgac gcggacgaga tcgaacgaga gcgctcggtc attttgcagg
      241 agatttccca ggcggaagac acccccgacg actacgtcca tgatcttttc aatctcgatt
      301 ttttcaggga tcatccaatc ggccggccga tttgcggccg agagcaaacc gtcaacaact
      361 tccggcgtga ggacatcatt gaattcttca agtcgcgtta tcagccacgc cgagtgatcg
      421 tgtcgatcgc cggcaatttt cagcacgcgg ctgtggtcga cgaaatggcg gcgcggctgg
      481 gttcggtgca ggaacaaaat cacgaccctc atccgggtgt caccggtgaa gcggttccgg
      541 agatgggcag tggaatattt ccgcacccca aatcgctgga acaggtgcat ctctgtctgg
      601 gcgtcgcggc aattccgcaa tcgcatccca aacgctacat cgcttatgtg ctcaacacgc
      661 tgctgggcgg cggcatgagc tcgcggttgt ttcaggagat ccgcgaaaag cgcggcaagg
      721 catactctgt atattccttc tcctccgcgt acaaagacgt gggctatctc ggcgtgtatg
      781 ccggcacgag cctggattca accgaagaag tcgtcgagct catcctccag gaattgaaaa
      841 agatggcggc cggcgacgtg agcgaaaatg agattgcccg cacacaaggt cagttggtcg
      901 ggagcatgat tttgggcctg gagtcgacgg attcatggat gagtcacgtc gcgcgcgatg
      961 aaatttattt cggccaagcg attggcatca atgaaatctg ccaaaaaatc cgcgccgtgt
     1021 cgcgggatga cgtgatcgag ctcgccgaca tgcttttcgc caagggtgcg atgacactga
     1081 cgctcttggg tgacttcgac gataagtttc agatcaaaaa attagatttg cctgcttgac
     1141 tcggtctcgg tttgaccatt cggcgggatt ctgtggaaca gcttcgtatc gagatcaaac
     1201 ggatacggcc cgcggccaat cagctggatc tgccggctta catgaccgag ggcgccgcag
     1261 gcatggacat ctgcgcggat atcgaccacg atcgcacgct cgcgcctctg gagcgcgccc
     1321 tgattcccac cggcgtcgcg atcgcgcttc cgccaggctt tgaggcgcaa attcggccgc
     1381 gcagcggcct ggctctacgg gagggtttga cgatcattaa tactccgggc acgatcgatt
     1441 cggattaccg tggcgagatt cagttgatcg cgatcaatct cggcagcgag ccgatcgtga
     1501 ttcgccgcgg gcagcgtatc gctcaaatga ttatacagcg agtggtgcgg gcggcatggg
     1561 tggaagtcga ggagctcccc gcttcagtga gagagtccgg cgggttcggg cataccgaca
     1621 agacacgcga ggagttagga gtgaggagta aggggcgctg acccctcacg acttacccct
     1681 cacgctaaat tttcatgatc ccccgttata cccgtccgga aatgggccgg atctggagcg
     1741 aagaaaatag ctttcaaaaa tggctcgacg tagagatcct cgcggccgag ggtctggcgc
     1801 gcatgggcag agtgccgaaa gccgcgattt cgcgcattcg aaaaaaagcc cgcctcgacg
     1861 taaagcgcat ccgcgccatc gaagccgagg tgaagcacga aaccattgcc ttcttgagcg
     1921 cggtggcgga aaccatcggt gacgatgcgc ggtatctgca cgtcggcatg acctcttcgg
     1981 atgtcatgga tacggcactg gcgatacagt tcaaggaggc ttcggcgatt ctcgaacagg
     2041 acatcaacga tctcatgacc gtgctgcggc gccaggcacg aaagtataaa tcgacggtga
     2101 tgatcgggcg aacccatgga gtgcacgcgg agccgattac tttcggcctc aagctcgcca
     2161 tctggtatca ggaaatggag cgccacctgt cgcgttttcg gcacgcggtg gaagacatct
     2221 gcgttgggaa aatttctggc gcggtgggca cgtttgcgca gattccgccc gaggtcgagg
     2281 cttacgtctg ccggaaaagc ggtctcaagc cggcaccggt atcgaatcaa attgttcagc
     2341 gggaccgtca cgctttttac ttttcgaccc tggcgctgat cgcgagctcg ctcgagaagt
     2401 tcgccatcga aatacgccac ctgcagcgca ctgaagtcca ggaggcggag gagccgttta
     2461 ctcgagggca aaagggctct tcggcgatgc cgcacaaacg gaacccgatt ctatcggaaa
     2521 acgtctcggg gctggcgcga ctgatgcgat cgtatgcgca tgccgcgatg gaaaacattc
     2581 ccctttggca tgagcgcgac atcagccact cgtcggtcga gcgggtcatc gcgccggatg
     2641 ccacaattgc cctcgacttc atgctgcatc gaatgaacta tgtgttgggc agtctatgtg
     2701 tctatccgga aaacatgcag cgcaatttag cgaagagcgg cggtgcggtt ttctcggaga
     2761 aggtgctgct cgctctggtg gaaaaaggtt tggcgcggga tgtcgcctat cgaatggtcc
     2821 aggcgcatgc gttgaaagtg ggaaaggaag gcggtgattt aaagcgcgag cttcttgcgg
     2881 acgccgagat tcgccgctat ttgccggctc aagagatcga caatttatgg ggtatcaagc
     2941 accacctggc aaacatcgat gtcattttcc gccgcgtctt tcgctgaatg gccgcgctta
     3001 gaaaacaaga cgatgcgtgt tagaattttt gtgtcgttga agaagggtgt tcttgatccg
     3061 caaggcaagg ctatcgagcg ctcactgcac gttttcgggt acaacgaagt ccaggatgtc
     3121 cgcgtcggca aatttctgga gctcgaagtg gatgcgacct cgcgcgaggg cgccgaggcg
     3181 cgggttcggg aaatgtgcga caagctgctc gccaatcctg tcatcgaaga ctaccgcttt
     3241 gaaataagtc ctgagttatg agtgctgagt tttcggataa acaagccgaa acgtctgcta
     3301 ctcaggactt agaactgaaa tgcaagaaag gactgaggac tgagctgtga ggactgaggt
     3361 ctcgaaaaga caagccggag cgtttgctat tgctactcag cactcggaac tcaggactca
     3421 gcactgatat gcgttggggt gtgattactt ttccgggttc gtgcgacgat cacgatgtca
     3481 tcgactgcct aaaaaccgat ttggggcaga aggtgaaggt gctgtggcac aaggacgagc
     3541 ttgccggcaa ttttgattgc ctcgtgctgc ccggcggatt ttcctacggt gattacctgc
     3601 gctgtggcgc gatggcgcgt ttttcgccga tcatgcgcgg ggtcatgaaa tttgctcaga
     3661 gcggcgggct cgtgctcggc gtctgtaacg gctttcagat tctttgcgag tcaggattgt
     3721 taccgggcgc tctggtgcgc aacagcgggc tgcgctttgt atgccagccg gttcatttgc
     3781 gggtggaaga aaccgacacg ccgtttacat cgaatcttaa aaaagggcag ctcctccgca
     3841 tgccagtcaa gcacggcgag ggttgctatt atgcggatgc taagaccctc gagagcttgc
     3901 gaaagaatcg gcaaatattg ctgcgctacg tcgatgccaa aggcaagccc acggccagcg
     3961 cgaatcccaa cggttctgtg gaaaacatcg ccgggatctg caacaagacg cggaacgtct
     4021 tcggtttgat gccgcacccc gaggatgctt gcagcgcgct tctcgggagt gaggacggcg
     4081 ccaagctatt catgtccatc gctgccgcat gcgccgtccg cgtgaacccg catgcttgaa
     4141 cctgcgggtc aggactttag tc
//
LOCUS       DASULZ010000703         5534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_588338, whole genome shotgun sequence.
ACCESSION   DASULZ010000703 DASULZ010000000
VERSION     DASULZ010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588338"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..508
                     /locus_tag="VFY96_14570"
     CDS             299..508
                     /locus_tag="VFY96_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607746.1"
                     /translation="MAKYLQCSECGKLAFYYDDYVGTEKPFYRRFMRYPEGETKYEPP
                     RCFTCGGVPRLIHENIKEEKAAAKH"
     gene            complement(555..1745)
                     /locus_tag="VFY96_14575"
     CDS             complement(555..1745)
                     /locus_tag="VFY96_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HEX5607747.1"
                     /translation="MINWLWLGSSMLLCLQWIFVHFFGVHLPPHLQGLTSGIAIFGAA
                     FALSWAAELSQKDIPQALAIALLALIAVLPEYAVDMYFAWTAGKDPSYAAFATANMTG
                     ANRLLIGVGWAAVVFTYWFTTHKRAVRLEPGHKIEIFYLALATLYSFLIPLKGQLDLS
                     DAAALILVFIFYMRAAAKAEHVEPELEGPAAMIESWGLGARRAMTTIFFLLSGFTIFI
                     AAEPFAEALLASGREYGIEEFILVQWLAPLASESPEFIVAILFALKAQPGISMGALLS
                     SKVNQWTLLIGMIPVAYAISAGHITGMQMDQRQVEEMFLTAAQSIFAVAVLANLSFSV
                     LEATIVFALFSTQLLFTDPQFRFYYSFLYVILAVGLVLLKKDARDGLCALLSRSSLGS
                     HVNR"
     gene            complement(2214..3050)
                     /locus_tag="VFY96_14580"
     CDS             complement(2214..3050)
                     /locus_tag="VFY96_14580"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX5607748.1"
                     /translation="MPRYLVLANQTAASPELTSVIRELVANDADTEFVLLVPATPVED
                     LLDWQEGDNETIAERTAQAAKDHLSGVGAKVARTEVGDASPVKAIENELERHQETYDG
                     IIISTLPLQRSRWVALDQPRRIERRFKVPVRHVVGHSVTMTREDLINGLNEDLNLELE
                     ALLRSVYHAAAGRGMLGHELRELLKKELPSELDHATFLADKIVALGGEVRIRPAMPAE
                     FSAARELLQQNIAAERKIIANYAKRIEQAAEFGDKGLAIRLENILASETDHLEQLERL
                     GR"
     gene            complement(3191..3430)
                     /locus_tag="VFY96_14585"
     CDS             complement(3191..3430)
                     /locus_tag="VFY96_14585"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HEX5607749.1"
                     /translation="MTDWFDDVRCTAFCLIEAPDGETIERAHNEAQSLIPNKIIEVEP
                     AVVEAFWAIQRSANVSRQNRVSIDAGFRAIMFTDL"
     gene            3808..4161
                     /locus_tag="VFY96_14590"
     CDS             3808..4161
                     /locus_tag="VFY96_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607750.1"
                     /translation="MKCSLRFALLACLLVTLGCSPLSTSEALTTREKAAGVGTVIGSG
                     VGAGIGSVWGYAVTGGGVGAALGLITGVLVGDHFQELEKKRSDLDRRIEECERELQRL
                     CDEVEKLKQQADEEQ"
     gene            4956..>5534
                     /locus_tag="VFY96_14595"
     CDS             4956..>5534
                     /locus_tag="VFY96_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607751.1"
                     /translation="MKQQIVLLAAMAALGIVSGNSGQSMGAESAQANPATSFFVSSSG
                     SKTANLGGLRGADKICQQLATAVGLGNKTWHAYLSAEHDPDNGNKPTDARSRIGTGPW
                     SNAKGVVVAKNLDELHARKGDADVFLDEHGQRVPGNWPNSPKPVVHDILTGSTADGKV
                     MAGKTCNDWTSASPDQQAQVGHSDGLGPGGDRS"
BASE COUNT         1356 a         1545 c         1457 g         1176 t
ORIGIN      
        1 ggcacttttt tcattgccag gacgaaccgg actttttcgc aaaacatcga tgtttgagac
       61 tgaaacagct taatcgccat aaaattcccc tcctaatact tttaggctcg gctgccgagc
      121 cgcgactttc ttagcaatgc aataaaatta atgatttctc aaccggtgca ggtagcgcca
      181 accgtggttg acccgatctg ggttggtcgc tatgctcctg ggcgtgggcg ggacaagcct
      241 gttactctgg taaaaccagc ggaccccccg atcactctga tctcaacgga aggaaaccat
      301 ggcaaaatat ctgcaatgca gcgagtgcgg caaactcgcc ttttactacg atgactatgt
      361 cggcaccgag aaaccgtttt atcggcgctt catgcgctat cccgaaggcg agaccaaata
      421 tgagccgccg cgttgcttta cctgcggggg tgtgcctcgt ttgatccacg aaaacatcaa
      481 ggaagaaaaa gccgcggcca aacattgagc tcttttccgc gaactcgatc gccgcgcaaa
      541 aatcacctta gcttttatcg atttacatgt gatcccagag atgagcgcga gagcaaagcg
      601 cacaacccat cgcgagcgtc ctttttaagc agcactaaac cgacggcgag gatgacgtac
      661 aaaaacgaat aataaaatcg gaactgcggg tcggtaaaca gcagttgggt cgagaaaagc
      721 gcaaagacta tcgtcgcttc cagcactgaa aaggataaat tcgccagcac cgcgacagca
      781 aagattgact gcgctgcggt caaaaacatc tcctcgactt gccgctgatc catctgcatg
      841 cccgtaatgt gacctgccga gatggcgtaa gcgacgggaa tcatgccaat cagcaatgtc
      901 cattgattga ccttcgatga taacaacgca cccatgctga tgcctggctg cgctttcaaa
      961 gcgaacagta tggcaacgat gaattcaggc gattccgagg ccaagggcgc aagccactga
     1021 accaggataa attcctcgat tccatactct cttccactgg ccagaagtgc ttctgcaaac
     1081 ggttccgcag caataaaaat cgtaaatccg gacaggagaa agaagattgt ggtcatcgct
     1141 cggcgcgcgc ccaaacccca gctctcgatc attgccgccg ggccctcgag ttctggctca
     1201 acgtgttcag cttttgccgc agcgcgcatg taaaatataa aaaccaagat taatgcggcc
     1261 gcatcagaaa gatcgagctg ccctttcaga ggaatcaaaa aagagtagag cgtcgcgagc
     1321 gccagataga aaatttcgat tttgtgtcct ggctcgagcc gaaccgctct tttatgggtt
     1381 gtaaaccaat acgtgaagac cacggccgcc catccgacgc cgatcaataa ccggttcgca
     1441 cccgtcatgt tggcggtggc gaaagccgca tagctgggat cttttccagc tgtccaagca
     1501 aaatacatgt cgacagcgta ttccggcaaa acagcaatga gtgccaacag agctatggcc
     1561 aaggcctgtg ggatgtcttt ttgcgagagc tctgcggccc acgacagggc aaatgccgcc
     1621 ccgaaaatcg cgatcccgga cgttaatcct tgcaggtgag gcggcaggtg aacaccgaaa
     1681 aaatgcacaa agatccattg cagacaaagc agcatcgacg agccgagcca aagccaattg
     1741 atcatccgtc ctcccgaact cacgccgaga aacttggtcc ctattttcat aggtaaaagt
     1801 ttaagtcaat ccaccaaaaa aggcgggtat tactcgcccg tctaattgag gcagtgagac
     1861 gacgacgacc aggggcagct caagggagcg ctgccaagtc cggcatatgt ggatcagagt
     1921 tatttgaaag aagcgttaaa gaatctgggc aacagatgaa ctgtggtatg aaaactaaaa
     1981 tgggcagaaa cactgctggc gcgccggtcc gcctcacagg caatgaattg taaattggca
     2041 ggcgcttgca aattgcaccg cacgcggccg ccaacgcctc tgatcccaac gactggcgcg
     2101 ctggtcgtct gcggctcact cagtgttgcg ccagaacgca attcgacaga attttgcccg
     2161 aaccgagcct cgcctgcgtc caaccccaaa gtcagacgag agcggtccgc ccactaacgt
     2221 cccagacgtt cgagctgctc gagatgatcg gtttcggaag ccaggatgtt ctccagccgt
     2281 atcgcgaggc ctttgtcgcc gaactccgcg gcctgctcga tccgcttcgc ataattggcg
     2341 ataatctttc gctcggccgc aatgttctgc tgcagaagct cccgcgccgc gctgaattct
     2401 gcgggcatgg caggccggat ccgcacctcg ccgccgagcg cgacgatttt gtccgctaga
     2461 aacgtcgcat gatcgagctc gctgggaagt tccttcttca acaactcccg gagttcatgg
     2521 ccgagcatgc cgcggcccgc cgctgcgtga tacacgctcc ttaaaagcgc ttcgagctcg
     2581 agattgagat cttcattgag accgttgatg agatcttccc gagtcatcgt cacggaatga
     2641 ccgaccacgt gcctgacggg gactttgaag cggcgctcga tgcgtcgggg ctgatcgagt
     2701 gcaacccagc gggacctttg cagtggtaaa gtggaaataa tgatgccgtc ataggtttct
     2761 tggtggcgtt ccagttcgtt ctcgatcgcc tttaccggcg aagcatcgcc tacttctgtg
     2821 cgcgcgactt tcgcgcccac tccgctgaga tgatcctttg cggcttgcgc ggtgcgctcg
     2881 gcgatggttt cattgtcgcc ctcctgccaa tcgagaagat cttccaccgg cgtggccgga
     2941 acgagcaaga cgaattccgt gtccgcatcg ttggcaacaa gctcgcgaat tacgctggtc
     3001 agctccggac tcgcggcggt ttggttggcc aaaacgagat agcgtggcat attcacacct
     3061 cccgagaatt tctcgccaaa aaataggccg aacgacgccc cgaatacaag ggttcccgct
     3121 tttgtggcgc cgcagaactc aatcaatccc gatgcacgca catcgctcta atggtcattc
     3181 gtctcgaatc ctacaaatcc gtgaacatga tcgcgcggaa acccgcatcg atcgaaaccc
     3241 tgttttgccg gctgacgttg gcggatcttt gaatcgccca aaaagcctcg accaccgcgg
     3301 gctccacctc gatgattttg ttggggatta aactctgcgc ttcgttgtgc gcgcgctcga
     3361 tcgtttcccc atccggcgct tcgatcagac aaaaagccgt acagcgaacg tcatcgaacc
     3421 aatccgtcaa aaatttacgt tgtatttctg ctggagcgtg agatccctct ggtgcgcatc
     3481 cgcgacggca tggcggattg cgccctcgac gtaatggcga tcaatgtaaa caggcatatc
     3541 tcgactcgaa tccttgaaat cgcgcaaaaa gctaactatc ggtcctgaac ccgatggaat
     3601 cataattgat tcctcgatca gtcaaccatg gcatggagcg ggggaagcac ttcagtatca
     3661 gcacaactac tttggtcttc tcatacttga gttgctgagc ggcaagattt accggttttg
     3721 caaattgttg gataattttt tccaacaata acgtacgccg gcagcggcat acactttgcc
     3781 ctgtttcatc ccggaaggaa gcccttcatg aaatgctcgc tgcggttcgc gctgcttgct
     3841 tgtctactcg ttactttggg ctgctcgccg ttgagcacct cggaagcgct cactactcga
     3901 gagaaagcgg cgggcgtcgg cacggtcata ggatccggtg tcggtgcggg aatcggcagc
     3961 gtatggggct atgccgtgac cggtggcgga gttggcgcgg ctctcgggtt gatcaccggc
     4021 gtgttggtcg gtgatcattt tcaggagcta gagaaaaagc gaagcgatct ggaccggcgc
     4081 atcgaagaat gcgagcgcga attgcagcgc ctgtgcgacg aagtagaaaa attaaaacag
     4141 caagccgacg aagaacaata acaactaatc cactcgccag gttctcaccg gcattccgcg
     4201 cctcgaaaat ctccgcttcg attcgcagtt cgcccgatgc tccgccgttt ggcctttcga
     4261 aaccagatgg gccccgctgg ccggaacctg gctcgatcca gagatccgag tcctgcacac
     4321 cgaaatttat ccgagcgtcc gagcaccgct cccggacacg atcaaggacc gcggccagtt
     4381 acgcgcgatg tggcactggg cgcgcgatct ccacgcaacg aactcactcg tcaaagaatt
     4441 cgcgatccct cacagtataa cgaacggctc agcagaggat cacatcatcc aatccaagga
     4501 gggctcgata ctagattgcg cacgttaatc gactagtaca tacacttcgt atctcatgtt
     4561 tcggaattcc gaaaacatgg gacggtccag tgatatattc gggtcaaatt cttgatccga
     4621 acgtgaccga ttactagatt atttgatggg gtttcgcgcg cgatgcgaaa gtgactcgaa
     4681 tcgggctcca acaattcgga attcgcgata agccgtctct caagcacacc cgacatacga
     4741 ttccatcttt tgtgggcgac tgttgatttc actgacgctt cacgcctcga actcttgcgt
     4801 catcgatcat gactgcgcca gtgctcgccg ggtcacccgt aaatcgagct aattcttgac
     4861 tccataaaga ggtgcggtgt agcgtgctcc gtgcttaagc cactggttaa cgaaaaacgt
     4921 ttcctgacaa aatatacagt ggaaaggaaa aacatatgaa gcaacaaatc gttcttcttg
     4981 cagcaatggc tgcgctagga attgtgagcg gtaacagtgg tcagagcatg ggcgctgaaa
     5041 gcgctcaggc caatcctgcc acgagctttt ttgtcagtag cagcgggagc aagacggcca
     5101 atctgggagg gctacgtggc gcagataaaa tttgtcagca gctggcgacc gccgtcggcc
     5161 tcggcaacaa gacctggcac gcctatttga gcgccgaaca cgatccggac aatggcaaca
     5221 agcctactga tgcacgcagt cgcattggca ccggtccgtg gtcgaacgcc aaaggcgtcg
     5281 tcgtggcgaa gaatcttgac gagctgcacg cccgcaaagg agacgcagac gtatttctcg
     5341 acgagcacgg ccagcgtgtt cccggtaatt ggccgaactc accaaaaccc gttgtccatg
     5401 acatcctcac cggctcgaca gcagacggta aggtcatggc cggaaagaca tgcaacgatt
     5461 ggacatcagc atctccggac caacaggcgc aggtggggca ttcggatggg ctaggacctg
     5521 gcggcgatcg gtcc
//
LOCUS       DASULZ010000704         6583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_181910, whole genome shotgun sequence.
ACCESSION   DASULZ010000704 DASULZ010000000
VERSION     DASULZ010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6583
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181910"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..1572)
                     /gene="radA"
                     /locus_tag="VFY96_14600"
     CDS             complement(211..1572)
                     /gene="radA"
                     /locus_tag="VFY96_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX5607752.1"
                     /translation="MAKAKIIFACQNCGYQSPKWLGKCPDCNQWNTFAEERFEKATTP
                     RGELSLGTKEDPAPIHRIDISEEGRVLSGIGEFDRVLGGGLVTGSVILIGGDPGIGKS
                     TLLLQAFAAVSQRSVTCLYVSGEESPRQIKMRAERLGIDAPNLLILSETSLERILDHI
                     KKIKPGLLVIDSVQTIFSSAIPSAPGSIAQVREASGSIIVLAKKTGLTTFLIGHVTKD
                     GAIAGPRLLEHMVDTVLYFEGERGHSFRILRAVKNRFGSTNEIGVFEMKEAGLQEVSN
                     PSEIFLMERPLAVPGSAVVCSMEGTRPILVELQALVSRSFLAVPRRTTIGVDHNRVAL
                     LVAVLEKKMGAKLFDQDIFVNVAGGVQVDEPAVDLGIVAAIASSSRETVLDARTIFFG
                     EVGLAGEIRGISQAEARVKEAGKLGFERCILPASNAAQLKYIEHPKLAAVSSLKECWK
                     LIF"
     gene            1985..2632
                     /locus_tag="VFY96_14605"
     CDS             1985..2632
                     /locus_tag="VFY96_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717365.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEX5607753.1"
                     /translation="MGKEENTSAAEFLPERRDLKSLREAARSCEGCDLYKNATQTVFG
                     EGAAHASIILIGEQPGDMEDRQGHPFVGPAGRMLDKALAEARISRDEVYITNAVKHFR
                     WIQRGKRRLHQKPAVRQIMACKPWLESEMASIHPKLLVCLGATAAQSILGRAVPIGQA
                     RGKFFNGQRGAAVFITIHPSAIFRQREKSEREEEYRRFVADMRLVKRKLRGMAAA"
     gene            2717..3895
                     /gene="bioF"
                     /locus_tag="VFY96_14610"
     CDS             2717..3895
                     /gene="bioF"
                     /locus_tag="VFY96_14610"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEX5607754.1"
                     /translation="MVAADIHAELEALRSAGLYRELRRVENQQGPVLRIDGREVINFS
                     SNNYLGIANHPALAEAAKQAIDRYGCGSGASRLISGNMTLHEELESRLAQFKGTEAAL
                     VFNSGFQANTGILATLAGEGDVILSDQLNHASIIDGCRLSRAQTLVYAHADLDQLEAN
                     LKNAQHARHRLIVTESIFSMDGDEVPLTEIVDLAERYDAMVMVDEAHATGVFGATGAG
                     VVSKHTLGDRVFVQMGTLGKALGGFGAYVAGSRALRELLINRCRSFIFTTALPPAIMA
                     RAIAATELIEREPERRKRLWENCGLLSDGLKRLGFGLKDFQSPIVPLIVGDAENCMKL
                     SQRLLEHGVFVQGIRPPTVPPGTSRLRITVMASHERWHIEKALEAFQISQNQNGGLFR
                     "
     gene            3876..5231
                     /gene="bioA"
                     /locus_tag="VFY96_14615"
     CDS             3876..5231
                     /gene="bioA"
                     /locus_tag="VFY96_14615"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003279879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEX5607755.1"
                     /translation="MADFSDNLKELDHSYLWHPFTQMQDWLGEEPCIISRAEGNFLID
                     VHGRKYLDGVSSLWCNVHGHRKKALDDAIRAQLERMSHSTFLGLSHVPGIQLAEKLIA
                     TAPRGLSRVFYSDSGATAVEIALKMAVQYWQLKNDTQRIQIASLAESYHGDTVGSMSV
                     GYTETFHRFHKSLLFPVLRITPPHVFRYYRCMSEADGLNAAIAEADEKIAANKNHLAA
                     LVMEPLMQGAAGMWAQPVEYVKALHGICRRYDILFILDEVATGFGRTGKMFACEHANV
                     VPDLLCLAKGMTGGYLPLAATLATEEIFSAFLGEYKEFKTFFHGHTYTGNPLGCAVAI
                     ANLEIFEREKTIERLPSRIAYMAARLEEFRRLAHVSDVRQWGYMVGIELVENKDSRKS
                     YAPEKRIGYKIILEARKRNLMIRPLGDVIVLMPPLSITDDELTSLIDIVYESIVEVTG
                     D"
     gene            5334..6044
                     /gene="bioD"
                     /locus_tag="VFY96_14620"
     CDS             5334..6044
                     /gene="bioD"
                     /locus_tag="VFY96_14620"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEX5607756.1"
                     /translation="MARSIFITGTDTGVGKTFVSCSLAALLRDFGYRIGVMKPVETGC
                     SRRDGQLFPEDAVRLKHASDCGAPIERICPYRFAEPLAPSIAAERTGDRIDIDHLLKL
                     HEEISSDHDVTLIEGAGGLMVPLLPSFTYADFARTAKLPLIVVAANRLGAINHLMLTL
                     EHALCKELKVIGYVLNCLTDQMSLASETNREVLASLTGVPCLGELPFVATAKSGSVFP
                     AEQFKKEFALRLIEPFLL"
     gene            6080..>6583
                     /locus_tag="VFY96_14625"
     CDS             6080..>6583
                     /locus_tag="VFY96_14625"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607757.1"
                     /translation="MLNSRRVFTILAGLLFCLSQNDGFAANQAIISYSSRSYAFLPAQ
                     VAVAQNFFRDENLEPLLIQMRSQVTVPALISGEVQYTLSFGNIIGSAMQGLPFKILAV
                     LTDKPLHSLVGRPEIKSINELRGKRIGTQRIGGSDQLAAEAILQAKGIDPKEVQFVTL
                     GGDEPVRV"
BASE COUNT         1485 a         1757 c         1861 g         1480 t
ORIGIN      
        1 agggttgccc ctaccggatt ttgagtccgg cgcgtctgcc attccaccac tccggcacga
       61 gaagtaatat tgataaccaa cactcgatta cgatgcaagt cgcatcgaaa ttgaaacctt
      121 caacgttcaa ggtcttttgg cgttgccgac gatctcaaac ggattgcccc ttggacttaa
      181 gcagggaagg ctgaaccgac acgcagatgc tcagaaaatc agtttccagc actctttgag
      241 tgagctgacg gcggcgagtt tgggatgctc gatatacttc aattgcgcgg cgttgctggc
      301 ggggagtatg cagcgctcga agccgagctt gccggcttcc ttgacacggg cttcggcttg
      361 agaaatgccg cgaatttcgc cggctaagcc aacttcgccg aagaagattg ttcttgcgtc
      421 gaggacggtt tcccggctgc tcgacgcaat cgcggcaacg atgcccagat cgaccgccgg
      481 ttcgtcgacc tgcacaccgc cggcgacatt gacaaatata tcctggtcga agagcttggc
      541 gcccattttt ttctcgagca cggcgacgag cagcgcgaca cggttatgat ccactccaat
      601 cgtcgttcgc cgcggcacag ctaggaacga acggctcacc aacgcttgca actcgaccaa
      661 aatgggccgg gtcccttcca tgctgcaaac aactgcggag ccgggcaccg cgagcggccg
      721 ctccattaga aagatttccg acggattgct gacctcctgc agtccggctt ccttcatttc
      781 gaaaacgccg atctcattgg tcgatccaaa acggttcttt acggcgcgca gaatacggaa
      841 actatggccg cgctctcctt cgaaatagag caccgtatcg accatatgct cgagcaggcg
      901 cgggcccgca atggcgccgt ccttggtaac atgaccgatc aggaatgtcg tcaagccggt
      961 tttttttgcg agcacgataa tcgagccgga ggcttctcga acctgcgcga tactaccggg
     1021 ggccgatgga atcgccgaag agaagattgt ttgcactgaa tcgatcacca gcagccccgg
     1081 tttaattttc ttgatgtgat cgagaattcg ctccaatgaa gtttcgctca aaatcaagag
     1141 attaggcgcg tcaataccga gccgctcggc gcgcattttg atctggcgtg gcgactcctc
     1201 gccggaaacg tacaggcaag tgacgcttct ttgactcaca gcggcaaacg cctgcaacag
     1261 caacgtcgac ttaccaatgc ccggatcgcc gccaatcagg atgacggagc cggttacgag
     1321 tccgccaccg agcacgcgat cgaactcgcc gatccctgat agcacacggc cttcttcgct
     1381 aatgtcgatc ctgtggatcg gcgcggggtc ctccttcgtg cccaagctca attcgccgcg
     1441 cggggtcgtt gctttttcga agcgttcttc ggcaaaggtg ttccactgat tacagtcggg
     1501 gcatttgccg agccactttg gcgattgata gccgcagttc tgacaggcaa agataatttt
     1561 ggctttagcc acgggattgc tcagagactt tcgccgaata aagttcgctg tctaaattct
     1621 cacactttaa tacggaattg tagggtgggt gtagacccac cgcgtcgaga cccatcgttt
     1681 gaggctgaac gcatcctacg ccagccgtag gcgaacaccg agcaaagatt tctcccgttg
     1741 gtcgaaatga caaatggttt ttgaatgcgt tgcagttagc gaacagaaat tgtcggatcg
     1801 gcgagggccg gggcgaggtc cgtggtagga tcttcagtat tgctacgctc acccatgcga
     1861 cctccgtcat atgcgtgaac accgaagaaa aatttctccg tccggtcgaa atgacaaatg
     1921 ctgctgatgg tccgcaggga atgctatcta gtgatcgcgg attcgcggtg ctagctttgg
     1981 acttatgggc aaagaagaaa acacctcggc agcagaattt cttcctgagc gccgggacct
     2041 taaaagcttg cgcgaggcag ctcgctcttg cgagggatgt gatctctata agaatgcgac
     2101 ccagacggtt tttggcgaag gggcggcaca tgcgagtatc attttgatcg gcgagcaacc
     2161 cggcgatatg gaagaccgtc aaggacatcc gttcgtcgga cctgcgggcc gaatgctcga
     2221 taaggctctg gctgaagcgc gcatctcgcg ggacgaggta tacatcacca acgccgtcaa
     2281 acattttaga tggatccagc ggggcaaaag acgcttgcat caaaaacctg cagtgaggca
     2341 aatcatggcc tgtaagccgt ggctcgaatc ggaaatggca tcgattcatc ccaaacttct
     2401 tgtatgcctt ggcgccaccg ccgctcaatc catccttggc agggctgtgc cgatcggcca
     2461 agcccgggga aaattcttca acgggcaacg tggcgcagcg gtatttatca cgattcatcc
     2521 ctccgcgatt ttccggcagc gagaaaaaag cgaacgagaa gaagaatacc gccgcttcgt
     2581 cgccgatatg cggctggtga agcggaagtt gcgcggtatg gcggctgcgt aattttgtta
     2641 tctttagaga tccgcagaat cacttctttg gtgccgtgtt gcacggcttc gggccgtata
     2701 ttatctaatt caggcaatgg ttgccgccga cattcacgcg gagcttgaag cgctccgcag
     2761 cgccggcctt tatagagaat tgcgccgcgt cgagaaccaa cagggtccgg tccttcgcat
     2821 cgacggccgc gaagtgatca atttttcctc gaacaattat ctgggcattg ccaatcatcc
     2881 agcgctggcg gaagcggcga aacaagcgat cgatcgttac ggctgtggct cgggcgcatc
     2941 acgcctgata tccggtaaca tgaccctgca tgaagagttg gaaagtcgcc ttgcgcaatt
     3001 taagggcacc gaggcggcgc tggtgttcaa ttccggtttt caggcgaaca cgggaattct
     3061 tgcgacgctc gcgggtgagg gcgacgttat tctcagcgat cagctcaatc acgcgagcat
     3121 catcgatggt tgccgattgt cccgcgcaca aactctcgtt tacgcccatg ctgatctcga
     3181 tcaactcgaa gccaatctca aaaatgctca acatgcgcgg caccgtctga tcgtcaccga
     3241 gtcgattttc agcatggatg gcgacgaggt accgctgact gagatcgtcg atcttgctga
     3301 aagatatgac gcgatggtga tggtggatga agcacatgcg accggcgttt tcggcgcaac
     3361 gggagccggc gtggtgagca agcatacgct gggagatcgc gttttcgtcc aaatggggac
     3421 tttgggaaaa gctctgggag ggtttggcgc atacgtggcc ggcagccggg cgcttcgcga
     3481 gctcttgatc aaccgttgcc ggagcttcat tttcacaacg gcgctgccgc cggcgattat
     3541 ggccagggcc atagccgcca ccgagttaat cgaacgtgaa cccgagcggc gcaagcgcct
     3601 gtgggaaaac tgcgggttat tgtcggatgg gttaaaacga ctcggcttcg ggttaaaaga
     3661 ttttcaaagc ccaatagtac cactgatcgt cggcgatgct gaaaactgca tgaaactttc
     3721 ccagcgactt ttggagcacg gggtttttgt tcagggaatc cgtccgccca cagtgccgcc
     3781 gggaacgtca cgccttcgga tcactgtaat ggccagccac gagcgttggc acatcgagaa
     3841 agcactcgag gctttccaga taagccaaaa tcaaaatggc ggacttttcc gataacctaa
     3901 aagaactcga tcatagctat ctctggcacc ctttcaccca gatgcaggat tggctcgggg
     3961 aggagccgtg tatcatcagt cgcgcggagg gcaattttct catcgatgta cacgggcgga
     4021 agtatctgga cggggtgtct tcgctttggt gcaatgttca cggccatcgg aaaaaggcgc
     4081 tcgacgacgc gattcgcgct cagctcgagc gcatgtctca ctcgacgttt ctcggcttga
     4141 gccatgttcc aggaatccaa ctcgctgaaa aattgatcgc gaccgcgccc agaggcttga
     4201 gccgggtttt ttattccgac agtggcgcga cggctgtcga aattgcgttg aaaatggccg
     4261 tgcaatattg gcagctcaag aatgacactc aacggataca gatcgcatca ttggccgagt
     4321 cctaccacgg cgacaccgtg ggatcgatga gtgtaggtta taccgagacg tttcaccgct
     4381 ttcacaaaag cctgctcttt ccggtgctgc ggatcacgcc gccgcacgtc tttcgttatt
     4441 accgctgcat gagcgaagcg gacgggctca atgcggcgat tgccgaagcc gacgagaaaa
     4501 ttgccgcgaa taaaaaccat ttggctgcgc tggtgatgga gccgttgatg cagggcgcgg
     4561 cgggaatgtg ggcgcagccg gtggagtatg tcaaggcgct acacggcatt tgtcgccgtt
     4621 acgatatctt gttcattctc gacgaagtcg cgaccggttt cggccgcacg ggaaagatgt
     4681 ttgcctgtga gcacgctaac gtggtccccg atcttctttg ccttgctaag gggatgaccg
     4741 gcggatatct gcccctggcg gcgacattgg cgaccgaaga aattttttcg gcgtttctcg
     4801 gtgaatataa agaattcaaa accttctttc acggacacac gtataccggg aacccgctcg
     4861 gctgcgccgt cgccatcgcc aatctggaaa ttttcgagcg cgaaaagacc atcgagcgct
     4921 tgccatcccg aatcgcctat atggccgcgc gactagagga gtttaggcgc cttgcccacg
     4981 taagcgatgt tcgccagtgg ggatacatgg tcgggatcga gctggtcgaa aacaaggata
     5041 gtcgcaagag ctatgcgccg gaaaaacgaa tcggctataa gattatcctc gaagcgcgca
     5101 agcgcaatct catgattcgt cccctcggtg acgtcattgt gttgatgcca cctctatcga
     5161 tcacagacga cgagctcact tcactgatcg atatcgtcta tgagagcatt gtcgaagtga
     5221 ccggcgattg atggaagcga aacaaactga ggatagagga tcgaagatgg aggatggcga
     5281 atcgataatc cattctcgat gttcgatgct cgctgtgagt ggcgaggcgt ctaatggcgc
     5341 gcagtatttt catcactggt acggacaccg gagttggtaa gacgttcgtc agctgtagct
     5401 tggcggcatt gttgcgtgat tttggctatc ggatcggcgt catgaagccg gtggagaccg
     5461 gctgttctcg acgggacggg cagctctttc cggaggacgc agtacgtctg aaacacgctt
     5521 cggattgtgg tgcaccgatt gaaagaatct gtccttatcg gttcgccgag ccgctcgcgc
     5581 cgagcatcgc ggctgaacga acgggcgaca ggatcgacat cgatcatcta ctgaagttgc
     5641 atgaagaaat cagctcggac catgatgtga ctctgatcga aggcgccggc gggctcatgg
     5701 tgccgctcct gccgagcttc acgtatgcgg attttgcgcg cactgctaaa ctgccgctca
     5761 tcgtcgttgc tgcgaaccgc ttgggagcga tcaatcactt aatgctgacc ttggagcacg
     5821 cgctgtgtaa agaattgaag gtcatcggct acgtgcttaa ctgtctaacc gaccaaatgt
     5881 cacttgcgag cgagacaaac cgagaagttc tagccagcct gaccggtgtt ccatgcctcg
     5941 gcgaattgcc ctttgtcgcg accgccaagt ctggaagcgt atttccggca gagcaattta
     6001 aaaaggaatt tgctcttcgg ctgatcgaac cttttctatt gtgactacga agcactcgaa
     6061 acgtttacga ggtgcgaatt tgctgaattc acgacgagtc ttcaccattt tggctgggtt
     6121 gcttttttgc ctttcacaaa acgatggttt tgccgccaac caggccatca tctcgtatag
     6181 cagtcggagt tacgcgtttt taccggcgca agttgcggta gcgcagaatt tttttcgcga
     6241 tgaaaatctc gagcctctct tgattcaaat gcgcagtcaa gtgactgttc cggcgttgat
     6301 cagcggcgag gtccagtaca cgttgagctt cggaaatatt atcggcagcg caatgcaagg
     6361 cttgccgttc aaaattctcg ctgtgctcac cgataagccg ctgcacagtc tggtaggccg
     6421 gcccgagatt aaatccatca acgagctgcg cggcaagcgc atcggcacgc aacgtatcgg
     6481 cggctccgat caactcgccg ccgaggcgat tctgcaggcg aaagggatcg atcccaaaga
     6541 agtgcagttt gtcaccttgg gcggcgatga accggtgcgc gtc
//
LOCUS       DASULZ010000705         2884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_689521, whole genome shotgun sequence.
ACCESSION   DASULZ010000705 DASULZ010000000
VERSION     DASULZ010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689521"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="VFY96_14630"
     CDS             complement(<1..517)
                     /locus_tag="VFY96_14630"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607758.1"
                     /translation="MAKRQLKYVGHNVPRVDGIDKVTGKAKFVGDLTVPGMLQGKILR
                     STYPHARVVSIEASQAEALPGVVAVLTASDIADLNLIYSGRPVIATDKVRYVGEPVAA
                     VAAVDLATAEEALSLIQVEYEELPAVVGIDAALKKDAPLIHRDKASNIGTHEKVARGD
                     IEEGFSLADEVV"
     gene            885..1853
                     /locus_tag="VFY96_14635"
     CDS             885..1853
                     /locus_tag="VFY96_14635"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX5607759.1"
                     /translation="MISAQDLKGALAIMPTPAKEGADRLDAINTVDLDETARLAESLV
                     RDGAAGIMALGTMGECATTAQNDYEAYVDCLLKTIRGRIPTFVGATALGGHEIARRIK
                     FIKERGATGTLLGIPMWQPATLDMAVKFYADVAQTFPDFPIMVYANPRAFRFAFDADF
                     WGPVVAKAPTVMSAKFSSKAILKKVVEVTRGRVNFVPPIGLAYEFAQISPETQTTCWI
                     PSVGPQVAIALMKALATRDAAQAKAVADDIAWANEPHHAITGSQEIFASYNIQLEKVL
                     MAASGYCKTGPIRPPYNVMPEEFRKAATEGGQRYAKLREKYSKLLG"
     gene            1873..2658
                     /locus_tag="VFY96_14640"
     CDS             1873..2658
                     /locus_tag="VFY96_14640"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5607760.1"
                     /translation="MKLDGQVAIIVGGARGIGEAIAHTFSKEGAKLVLVDLEKMKPPL
                     DAAVQAINQQGGNAVAVTADCSDDRQVNALVDEGVRRFGKIDILINSAGFRGPLVPVT
                     EISEKEFDDVILYNLKLVFLCCRAVLKQMVKQKNGSIVSISGTAGREGMAMRGSLCAA
                     KWGVTGLTQTIAKEFGPYGIRANLICPGGMDEPDLREMYAQRAKNLGMSIEAHEAQIL
                     ELTPLRKYAKHDEVAKAALFLASSDSSHTTGESLNVSGGRLMS"
     gene            complement(2705..>2884)
                     /locus_tag="VFY96_14645"
     CDS             complement(2705..>2884)
                     /locus_tag="VFY96_14645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607761.1"
                     /translation="VVCCQHGWWQECKELELRGYDSYGAGSANPSLLIGSDLADPISG
                     SLPHRSFLCRVKPAL"
BASE COUNT          630 a          853 c          820 g          581 t
ORIGIN      
        1 cgaccacctc gtcagcgaga gagaatcctt cttcgatatc gccgcgcgcg accttctcgt
       61 gcgtgccgat gttgctggcc ttgtctcggt gaatcagcgg cgcgtccttc ttcagtgcgg
      121 cgtcgatccc gacgaccgca ggaagctcct cgtattccac ctgaatcagc gacaacgctt
      181 cttccgccgt cgccaaatcc acggcggcga ccgccgccac cggctcgccc acatagcgta
      241 ccttgtcggt cgcgataacc ggccgcccgc tgtaaatcaa gttgagatcg gcgatgtcgg
      301 aagcggtcaa taccgcaacc acgccgggca gggcttccgc ctggctcgct tcgatcgata
      361 ccacgcgtgc gtgcggataa gtgctgcgga gaattttgcc ctgcagcatt cccggaaccg
      421 ttaaatcacc gacaaatttc gccttgccgg tgactttgtc gatgccatcg acccgaggca
      481 cgttatggcc gacatatttg agttgccgct tcgccactaa tctcctgcgc aaaaaatgta
      541 tgtcgaccct aacaaagcaa tttttggact tcaagaaagg accgagacgg ttggaatttt
      601 tgaaggccaa gcacgcaatc ttcgctgtcg atttgtcatt tcgagctgcg actgcggcta
      661 ttttcgtccc gccgacgaag tcgatgccgc gtacggccgc tcgcttcgcc gcgcggtcag
      721 agtcggaatc gaacgactag cctatgtcgc cctggtgact ccaaaaagga ttatagttac
      781 cgcaaaactg gagacgaggc tgtgagccga ccggattgcg gcgcgcggac tccccatgtt
      841 agacaaggac tggtcacagc aaattctcgt ccaaggaggt gctcatgatt tcggcccaag
      901 atctgaaagg cgcgctggcc attatgccca cgccggcaaa agaaggcgcc gatcgtctcg
      961 acgcaatcaa cacggtggat ctcgatgaga ctgcgcgcct ggccgagagt ctcgtccgcg
     1021 acggcgccgc ggggatcatg gcgctcggca ccatgggaga atgcgcgacc acagcgcaaa
     1081 acgattatga agcctacgtt gattgcttgt taaaaactat ccgtggccgc attccgacat
     1141 tcgtcggcgc caccgcgctg ggcggccatg aaatcgcgcg gcgcatcaaa ttcattaaag
     1201 aacggggcgc caccggcaca ctcttgggca ttccgatgtg gcaaccagcc actctcgaca
     1261 tggcggtgaa gttttacgcc gatgtcgcgc aaacatttcc ggactttccg atcatggttt
     1321 atgccaaccc acgcgcgttt cgcttcgcct tcgatgcgga tttctgggga ccggtcgtcg
     1381 ccaaggcacc caccgtcatg tcagccaaat tttccagtaa agcgattcta aagaaagtgg
     1441 tcgaggtcac ccgcggtcgc gtgaacttcg tgccgcctat cggactcgcc tatgagttcg
     1501 cgcagatttc gcccgaaacc cagactacct gttggattcc gtcggtcggg ccgcaggtcg
     1561 ccatcgctct gatgaaggca ctggcgacgc gtgacgcggc gcaagccaag gccgttgccg
     1621 acgatatcgc ctgggccaat gaaccgcacc acgcgatcac cgggtcgcag gaaattttcg
     1681 cctcctataa cattcagctc gaaaaagtct tgatggccgc ttccggttac tgcaagaccg
     1741 gcccgatccg gccgccttac aatgtgatgc cggaggaatt ccgcaaagcg gccaccgaag
     1801 gaggccagcg ctacgccaag ttgcgcgaga agtattcaaa gctgttgggt tgatgggatc
     1861 cgaggagcag aaatgaagtt ggacggtcaa gtcgcgatta ttgtcggcgg tgcgcgggga
     1921 attggcgaag ccatcgcaca cactttttca aaggaaggcg cgaagctcgt cctagtggat
     1981 ctcgaaaaaa tgaagccgcc gctcgacgct gccgttcaag cgatcaatca gcaaggcggc
     2041 aacgcggttg cggtgaccgc cgattgcagc gatgatcggc aagtcaatgc tcttgtggat
     2101 gaaggcgtac gccgcttcgg caaaatcgat atcctgatca acagcgctgg ttttcgcggt
     2161 ccgctggtgc cggtaaccga gatcagcgag aaggaattcg acgacgtgat tctctacaac
     2221 ctgaagctcg tctttttatg ctgccgcgcg gttttaaagc agatggtcaa acagaaaaac
     2281 ggcagcatcg tcagcatttc cggcaccgcg ggtcgcgaag ggatggccat gcgtggctcg
     2341 ctctgcgctg cgaagtgggg cgtcaccgga ctgacgcaga cgatcgccaa ggaattcggt
     2401 ccgtacggca ttcgtgcgaa cttgatctgc cccggcggca tggacgagcc ggatttgcgc
     2461 gagatgtacg ctcaacgcgc aaagaactta ggcatgagca tcgaagcgca tgaagcgcaa
     2521 attctcgagc tcactcctct gcgcaaatat gccaagcacg acgaagtcgc caaagctgct
     2581 ctattcctcg cctccagcga ttcttcgcac acgaccggag aatcgctcaa tgtttccggc
     2641 ggacgattga tgagctgagc cggactaccg tcgctctcgc ggtgataccg aaaacttttc
     2701 gcccttacaa agccggtttc acacggcaca agaaggatcg atgcggcagc gagccgctga
     2761 tcgggtcggc gaggtcggag ccgatcagaa ggctgggatt ggctgatccg gcgccgtacg
     2821 aatcgtagcc tcgcagctca agctctttgc attcctgcca ccagccgtgc tgacaacaca
     2881 ctac
//
LOCUS       DASULZ010000706         4128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_384084, whole genome shotgun sequence.
ACCESSION   DASULZ010000706 DASULZ010000000
VERSION     DASULZ010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384084"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /locus_tag="VFY96_14650"
     CDS             <1..1175
                     /locus_tag="VFY96_14650"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5607762.1"
                     /translation="GLEWELAMVWLVNKQIATLERAEIWQHPALKLSHDATQLQLARG
                     VGLPGRVWASEEATWVSDLTKEAEVMRRGIPGDGTLHGAFAFPIKIRGEVLGIVVFVS
                     TMVRAPDDDLLNMAQALGGEIGQFVERMRAEAALRQSEEHLRNQAQELEQQLLASGRL
                     VAVGELTASMAHEFNNPLGIVLGFAQGLLANMDSSDPNYHHVQIISEEAKRCEKLVQE
                     LLEFGRPKNADFAWTDVHEMIQKTLDLVQPRAGKSKIEAYAKFEGELPQIHADPQQLQ
                     QVLLNLSLNAVDAMPKGGILTIGAKQESPNGLTITVADSGIGIEPDVLPRIFQPFFTS
                     KKRRGLGLGLPICDRIVKAHGGRIEVESISGEGTRFTIRLPINGSTETSARQETMH"
     gene            1509..3419
                     /gene="glgB"
                     /locus_tag="VFY96_14655"
     CDS             1509..3419
                     /gene="glgB"
                     /locus_tag="VFY96_14655"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEX5607763.1"
                     /translation="MSATVSLLGDQDFYLFNAGSHTRLYEKLGAHQITSDGRDGTYFA
                     VWAPNADAVFVFGTFNDWNKASHPLRPKGSSGIWEGFVPGVGQGTLYKYFVHSRFMGY
                     RVEKADPFAIFNEIPPKKASIVWNLDYSWNDGQWMQERKSRSALDKPISTYEVHLGSW
                     RRIPEEGNRSLSYRELAPALSEYVRQMGFTHVEFLPLMDHPFFGSWGYQITGYFAPSG
                     NYGTPQDLMFLIDTLHQNGIGVILDWVPSHFPADEHGLAYFDGTHLFEHADPRQGYHP
                     EWNSYIFNYGRHEVRSFLASNALFWLDKYHVDGLRVDAVASMLYLDYARQPGEWVPNK
                     YGGRENIEAIEFLKELNGSIYGSDPDVQTIAEESTAWPMVSRPVYAGGLGFGLKWDMG
                     WMHDTLAYFSQEPIYRKYHHDKLTFRMIYAFHENFVLPLSHDEVVYGKGSLLGKMPGD
                     EWQRFANLRSLFGYMYAQPGKKLLFMGGEIAQWSEWQHDQSLDWLALNSPQHAGMRRW
                     VQDLNWFYRNELALHELDCDPAGFEWIDCRDADSSVVILLRKSRRQEQLVLVACNFTP
                     VPRLNYRIGAPIGGMWKESLNSDAKDYGGSGMGNMGEVEAAPVPCHGRRFSLPLTLPP
                     LSTVFLTHTVNV"
     gene            3509..>4128
                     /locus_tag="VFY96_14660"
     CDS             3509..>4128
                     /locus_tag="VFY96_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009283567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX5607764.1"
                     /translation="MIEEKPSRVGAEYLGQNRCRFRVWAPELAPIEVRLLNTNREVRM
                     EQTARGYHEALVSGVEPGERYFFRLPENKERPDLASRSQPDGVHGSSQVVAQDFAWND
                     KSWRGLALEDYIIYELHVGLFTAAGSFEAIIPQFDYLVELGITAIELMPVGQFPGRRN
                     WGYDGVFPFAAQNSYGGPEGLKKLVDACHRRGLAVILDVVYNHLGP"
BASE COUNT          947 a         1060 c         1184 g          937 t
ORIGIN      
        1 aaggtctcga gtgggaactg gcgatggttt ggcttgtcaa taagcaaata gcgacgttgg
       61 agcgggccga gatttggcag catccagcgt tgaagctttc acatgacgca acacagttac
      121 agctcgcgcg cggtgtcgga cttcccgggc gcgtatgggc cagtgaagaa gcgacgtggg
      181 tctcggacct aacgaaggaa gcggaggtga tgcggcgcgg tattccggga gacggtacgc
      241 tgcacggggc gttcgccttt ccaattaaaa ttcgggggga agtcctcggc atagtggtat
      301 tcgtcagcac catggtgcgt gcgccggatg atgatctatt gaacatggca caggcgcttg
      361 gcggggaaat cggacaattt gtcgagcgca tgcgcgcaga ggctgccttg cgccagagcg
      421 aagaacattt gcggaatcaa gcgcaggagc tcgagcagca actactggca tcgggccgtc
      481 tggtggcagt gggcgaactc accgcctcaa tggcgcacga attcaacaat ccattgggaa
      541 tcgtgctcgg atttgcccag ggcctacttg cgaatatgga ctcttcagat cccaattatc
      601 atcatgtgca aattatctcc gaagaggcca agcgttgcga aaagttggtt caggagttgc
      661 tcgaattcgg gcgtcccaaa aacgccgatt tcgcatggac ggacgtccac gagatgattc
      721 agaaaacgct agaccttgtc caaccgcgag cggggaaaag taaaatagag gcgtatgcca
      781 aattcgaagg agagctgccg caaatacacg ccgatccgca gcagttgcaa caggttttgt
      841 tgaatctctc gttaaacgcc gtggatgcga tgcccaaagg cggaatcctc accatcggcg
      901 ctaaacagga gtcgccgaat ggcctgacga ttactgttgc ggacagcggc atcggcattg
      961 aaccggacgt gctgccgcgg atttttcagc cgttctttac gtcgaaaaag agacgcggct
     1021 tgggtttagg tctgccgatc tgtgatcgca tcgttaaggc ccatggcggc cggatcgagg
     1081 tcgagagcat ttcaggggaa ggcacgagat ttaccattcg cctgcctatc aatggatcca
     1141 ctgaaacgag cgcgcgccaa gaaaccatgc actgagctag ccttcagaaa agtcttcggc
     1201 gaacgccttt ctccggctta agcgtcaaag tctgcagcct cgagaaatcg ggtttcctac
     1261 atggccctgt attacgtcgt tacggaacga catgtggtct cttttttgcg ttttcactgt
     1321 tgacaattta tttctcgata gtcgaatcaa tgacgggtac agcggccacc atgcgtttga
     1381 taattagata aatcacatcg ccttgtattc cagaagggcg tccgtcacgt tatggtaaac
     1441 acatgagccc gcaaaccacc gagcgaaatc atgggggacg atcgacttcc tcaacgaaac
     1501 gagccgaaat gtcggctaca gtgagcttgt taggcgatca ggatttttat ctgttcaatg
     1561 ccggtagcca tacgcggttg tacgagaaat taggcgcgca ccaaatcaca tcagatggac
     1621 gagacggaac ttactttgcg gtttgggcgc ccaatgccga cgcggttttc gtcttcggta
     1681 catttaacga ttggaataaa gcgagtcatc cgcttcgtcc caaagggagc tccgggattt
     1741 gggaaggttt tgttcccggc gtaggacagg ggacgctcta caaatatttc gttcattccc
     1801 gctttatggg ttaccgggtc gagaaagccg atccatttgc aatctttaat gagattccgc
     1861 ccaagaaagc atcgatcgtg tggaatctcg attacagctg gaacgacggc cagtggatgc
     1921 aggaacggaa atcacggagc gccttggaca aaccgatctc aacatacgag gtgcatctcg
     1981 gttcgtggcg gcgcattccc gaagaaggca accgctcgct ctcttaccgt gagttggcgc
     2041 ccgcgttaag cgaatatgta cgccagatgg gctttaccca tgtcgaattt ctgcctttga
     2101 tggaccatcc atttttcggt tcttggggtt atcaaattac cggctatttt gcgccctcgg
     2161 gaaattacgg cacgccccag gatctgatgt tcttgatcga cacacttcat caaaacggca
     2221 tcggcgtgat tctcgactgg gtgccatccc attttcccgc agatgagcac gggctagcat
     2281 atttcgacgg cacgcatctc ttcgagcatg ccgatccgcg ccagggctat cacccggaat
     2341 ggaacagcta cattttcaat tacggccgcc acgaagtgcg cagtttcctg gccagcaatg
     2401 cgcttttttg gctggataaa tatcacgtgg acggtctgcg cgtcgatgct gtcgcatcca
     2461 tgctctatct cgattatgca cgccagcccg gcgagtgggt tcccaataag tacggcggca
     2521 gggaaaacat cgaagccatc gaatttctca aggaactgaa cggctcgatc tatggaagcg
     2581 accctgatgt gcaaaccatc gctgaagagt ccacggcatg gccgatggtg tctcggccgg
     2641 tatacgcggg cgggttggga ttcggcctga aatgggacat gggctggatg catgacacgt
     2701 tggcctattt ttcccaggag ccgatttacc gaaaatatca ccacgacaag ctgacattca
     2761 gaatgatcta cgcctttcat gaaaattttg ttttaccgct ttcccacgac gaagtcgtct
     2821 acggcaaggg atcgctcctc gggaaaatgc ccggcgacga gtggcagcgc ttcgcgaatt
     2881 taagatcgct gttcggctat atgtacgctc agcccggaaa aaagctgctg ttcatgggcg
     2941 gtgaaatcgc tcaatggtct gaatggcaac acgatcagag tctcgactgg cttgcgctga
     3001 acagcccgca gcatgccggg atgcgccgtt gggtgcaaga tctgaactgg ttttatcgaa
     3061 atgagctggc gctgcacgag ctcgactgtg accctgcagg atttgaatgg atcgattgcc
     3121 gagatgcgga ctcaagcgtg gtgatcctgc tgcggaaaag ccgtcgccaa gaacagctcg
     3181 tgctggtcgc ctgtaacttt acgcccgtgc cgcgcctcaa ctatcggatc ggcgcgccga
     3241 tcgggggcat gtggaaggaa tcgctcaaca gcgatgcaaa ggattacggt ggcagcggca
     3301 tgggtaatat gggcgaggtg gaagccgcgc cggtcccgtg tcatggacga cgtttttcac
     3361 tgcctctgac cttaccaccg ctgtccacgg tttttttgac tcatacggtt aacgtttagc
     3421 agagacatcg atgcggcgct gcatcagttg gcgacacgcg cccatgcaga cgagaggatg
     3481 cccaactttt ttgggtagtt gagaaattgt gattgaagag aaaccaagca gagtcggagc
     3541 ggaatattta ggccagaatc gctgtcgctt tcgcgtctgg gcgccggaac tcgcgcccat
     3601 tgaagtgcgg ttgttgaata ctaaccggga agtccgcatg gaacagaccg cgcgcggcta
     3661 tcacgaagcc ttggtttccg gcgtggagcc aggcgagcga tatttttttc gcttgcccga
     3721 gaacaaggaa cgccccgacc tcgcgtcgcg ctcgcaaccg gacggcgtgc atggatcttc
     3781 gcaggtggta gctcaggatt ttgcatggaa cgacaaaagc tggcgcggct tagcgcttga
     3841 agattatatt atctacgagc tccatgtcgg tctctttacc gcggccggaa gcttcgaggc
     3901 gatcattcca caattcgatt atctcgtcga actcggtatt accgcaatag aactcatgcc
     3961 ggtggggcaa ttccccggcc ggcgaaactg gggctatgat ggggtgtttc cattcgccgc
     4021 gcagaattcc tacggcggcc ctgagggatt aaagaagctc gtcgatgctt gtcaccgacg
     4081 cgggctagcg gtcatcttgg atgtcgttta caaccatctc ggcccgga
//
LOCUS       DASULZ010000707         2901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1501432, whole genome shotgun sequence.
ACCESSION   DASULZ010000707 DASULZ010000000
VERSION     DASULZ010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501432"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..867)
                     /locus_tag="VFY96_14665"
     CDS             complement(85..867)
                     /locus_tag="VFY96_14665"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX5607765.1"
                     /translation="MRFMFLNHSYKRHSAELERHILELLKSYAAPDTMFELAYPEDLG
                     GGEVLSLLEERKALSGLHHILETPALVKKAIDAERRGFDAVIQSNTFDPGVEASRLAV
                     RIPVIGLLRASLHFAATICDHVGLIVPLETHMPHTMRLVRTYGMDGFVSGIRTVGLYD
                     TGDLSGYHDVVIERTLAVGKDLVSQGAEAIIPLGGKIYPYVVTPEELEPELGVPVINT
                     KAVGVSYAELMARNNIKHSIKAYPCPTGLDPEAVSQRSKDTR"
     gene            complement(912..1913)
                     /locus_tag="VFY96_14670"
     CDS             complement(912..1913)
                     /locus_tag="VFY96_14670"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5607766.1"
                     /translation="MFGKIYLGLWIYAALFCMPASAAVSDRLIDSAKREREVVYYASM
                     NLSEANVLIGEFEKRYPFIKIKLNRAASEKLLTRVLAESRAQRSFADVIQTVEFSMHI
                     FARRGILARYTPQDNALFPKQFKEEGFWTTAYYNAYVTGFNTKLVAGKSLPRTYDDLL
                     DPKWKGKLMMEGTKADWFAGMLQIMGAERGISYMRALAQQQPSLREGHELLAQLVAAG
                     EGLLDVNIPAASVERMKERGAPIDWTALGPAPAIMVGIGQAAQATHPNAAKVFLDFVL
                     SRDGQKLMQTPGRLVARSDLANDQARMLKGLQIVPVDPALAEKLDQYARQLRSIFAG"
     gene            complement(1925..>2901)
                     /locus_tag="VFY96_14675"
     CDS             complement(1925..>2901)
                     /locus_tag="VFY96_14675"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607767.1"
                     /translation="TAAKTEPFRRVMTELIPLSAGFATIGQGAGPMMVTWKAGLFHKH
                     GLAVERPRIMGGAKGVVRGLLLGEIQFGNLAAPAALRANLKGEADLVFLTGGINQQFL
                     LGRPGITNRQQLAGRRIGFVGDGGLNDALVKFIIEQLTAAGIMDVRETTLPGGGDKEI
                     EALLRGECDAIVSTPPECISAHRQGCQYLVDFADYGLNYALGGIAARRSYVEENLEIT
                     KKFIKAYVEGMHRYRTDRDFTIEVQAEYSGIADRSIAEETYDLTRPGMPAMPYPVVSS
                     LQTLLNFMAREVPEAQNVHAGSFVDERFIRELEESGFIAALGDARVSQ"
BASE COUNT          622 a          831 c          802 g          646 t
ORIGIN      
        1 gatccagaat gggcgtatgc ctacgtccct acggatttcc gaatccgagt cgtgaatgat
       61 cgaatgcatt ttagttcgag gacactagcg agtatctttc gagcgctgtg agacagcttc
      121 gggatcgagc cctgtcggac acggatacgc tttgatgctg tgtttgatgt tgttgcgtgc
      181 catcaactcg gcgtagctga cgcccaccgc cttggtgttg attaccggta cgccgagctc
      241 cggttccagc tcctccggtg tgacgacgta aggatagatt ttgccgccga gcgggatgat
      301 cgcttcggcg ccctgcgaga cgaggtcttt accgactgca agcgtgcgct cgatgacaac
      361 atcatggtag ccagaaagat cgccggtatc atagagtccc acggttcgga tgccggaaac
      421 gaagccgtcc atgccatagg tgcggaccaa gcgcatcgta tggggcatat gggtttcgag
      481 cggcacgatg aggccgacgt gatcgcaaat cgtggccgca aaatggagcg atgcgcgcaa
      541 tagcccgatc acgggaatgc gcaccgccag gcggcttgct tcaacaccgg gatcgaaggt
      601 gttgctttga atcaccgcat cgaatccgcg tcgctcggca tcgatggctt ttttgaccag
      661 cgctggggtt tcgaggatgt gatgcagtcc cgagagcgcc ttacgctcct cgagcaggct
      721 taacacttcg ccgccgccga gatcttccgg gtaggcaagc tcaaacatcg tgtccggcgc
      781 ggcatagctc ttgagcaact caagaatgtg acgttcgagc tctgccgaat ggcgcttgta
      841 ggaatgattc aaaaacatga agcgcatcgt tccacctcct caatcaattt ctgagcgatc
      901 ctgccaaatc ctcatccagc aaaaatgctg cgcaactgtc tggcgtattg gtccagtttt
      961 tccgcgagag ccggatccac cggcacgatc tgcagccctt ttagcatgcg cgcctgatcg
     1021 ttcgcgagat cgcttcgggc caccagccga cccggggttt gcatcagctt ttgcccatca
     1081 cgcgaaagca cgaagtccaa aaaaactttc gccgcgttag gatgagtcgc ttgggcggct
     1141 tgaccgattc cgaccataat cgcgggtgca ggacccagcg ctgtccaatc gatgggagca
     1201 ccgcgctcct tcatgcgttc aaccgaggcc gccggaatat tgacatcgag gagaccttcg
     1261 ccggcagcga cgagctgagc caagagctca tgaccttcgc gcaaactggg ttgctgttgt
     1321 gcgagcgcgc gcatgtaact gatgccgcgt tctgcgccca tgatttgcag catcccggca
     1381 aaccaatcgg ccttggtgcc ttccatcatg agtttgcctt tccatttggg atcgagaagg
     1441 tcgtcatagg ttctcggcaa actcttgccc gcaacgagct tcgtgttaaa gcccgttaca
     1501 tacgcattgt agtaggcagt ggtccaaaac ccctcctcct taaactgctt ggggaagagc
     1561 gcattgtcct gcggcgtgta gcgcgccaga atgccgcggc gcgcgaaaat gtgcatgctg
     1621 aattcaaccg tctgaatgac gtcggcgaaa cttctctggg cacgggattc tgccaagacg
     1681 cgggtcaaga gcttttcgct cgcggcgcgg ttgagtttga ttttgatgaa gggataacgt
     1741 ttttcaaatt caccgatcag gacattggcc tcgctgaggt tcatggacgc gtagtagacc
     1801 acttcgcgtt cccgcttggc cgaatcgatc aagcgatcac ttacggcagc ggacgctggc
     1861 atgcaaaaga gcgccgcata gatccagagg ccgagataaa tttttccaaa catgttcagt
     1921 gtgtctattg gctcactcga gcgtcgccaa gtgcggcgat gaacccgctt tcctcgagct
     1981 cccggatgaa gcgctcgtca acgaaactgc cggcatgaac gttttgcgct tccggaactt
     2041 ccctggccat aaaatttagc agtgtctgta aggacgaaac caccggatag ggcatagccg
     2101 gcatgcccgg acgagtgaga tcataggttt cctcggctat gctgcgatcg gcaataccgc
     2161 tgtattcggc ctggacttca atggtaaaat cgcgatcagt tcgatagcga tgcatgcctt
     2221 ccacgtaggc tttgatgaac tttttcgtaa tctcgagatt ctcctcgaca taactgcgcc
     2281 gcgctgcgat tccgccgaga gcgtagttca agccgtaatc ggcgaaatcg accaaatact
     2341 ggcagccctg cctgtgcgca ctgatgcatt ccggcggcgt gctgacaatg gcgtcgcatt
     2401 caccccgcaa aagcgcttcg atctctttat cgccgccccc aggaagcgtt gtctctcgga
     2461 catccataat gccagcggca gtcagttgct cgatgatgaa tttcactagc gcgtcattga
     2521 gaccaccatc accgacaaaa ccgattctcc tcccagccaa ttgctgccgg ttagtaatgc
     2581 ccggacgtcc aaggaggaac tgttgattga taccgccggt caaaaagacc agatcagctt
     2641 cgcctttaag atttgccctc agggccgcgg gggctgcgag attgccaaac tgaatttcgc
     2701 ccaacagaag tccacgcacc acgcccttgg caccgcccat gatgcgcggc cgctccacgg
     2761 ccagcccatg cttatgaaag agcccggctt tccaggtcac catcatcggt ccggcgcctt
     2821 ggccgatggt ggcaaagccg gcagagagcg gtatgagctc cgtcatgact ctcctgaaag
     2881 gctcagtttt ggccgctgtt g
//
LOCUS       DASULZ010000708         2860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_81767, whole genome shotgun sequence.
ACCESSION   DASULZ010000708 DASULZ010000000
VERSION     DASULZ010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81767"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VFY96_14680"
     CDS             <1..308
                     /locus_tag="VFY96_14680"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607768.1"
                     /translation="IIGNTTNKLIFSIYVRPEIKTIEDLKGKKIGVTRFGSATDIAAR
                     FALRKHNLDPQKDVTILQMGAMTSIMGGLQGGSIDAGLVSPPTLFAGRKWALKSSSA"
     gene            281..694
                     /locus_tag="VFY96_14685"
     CDS             281..694
                     /locus_tag="VFY96_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607769.1"
                     /translation="MGFKELVSMTDLDLAFPNPSLVVQGGILRKKPESVDGFMRAYAR
                     GIQRARTDKEFALKSIANYTKIEDPAVLQKAYDLYVGKVLEKAPYINMVGMRNALADL
                     AKTVPAAKDVKPEQFIDMQFLDRLEQSGLLKKLYP"
     gene            complement(1583..2074)
                     /locus_tag="VFY96_14690"
     CDS             complement(1583..2074)
                     /locus_tag="VFY96_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607770.1"
                     /translation="MYKQLARVLHPDLELDPERRRQKERLMQELTVAYSNNDMHTLLR
                     LEMEWIQREEGEIERLTEEKLAVYNQTLKEQVQGLEQELGELPCHPRYEPIAVFQEPF
                     RATLRDGGPAEAFRLDQANAGIEASVRDLRSSNGLEILKAILRSYRQEQRASRWDLRD
                     FAF"
     gene            2134..2382
                     /locus_tag="VFY96_14695"
     CDS             2134..2382
                     /locus_tag="VFY96_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607771.1"
                     /translation="MARRFTARPRRKRLLLFLNLLLQTSLHFGGLDSQCLVAHYRLHI
                     GKIDIDAELVKHRLHFLPRFFKLLARNLFEVLSAYALK"
     gene            complement(2573..2779)
                     /locus_tag="VFY96_14700"
     CDS             complement(2573..2779)
                     /locus_tag="VFY96_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607772.1"
                     /translation="MSEQISLFNDGAFRVEAKFDELTLVAPRGRPLTKAQQTFNRLIK
                     RISRKVASVRARALRNSVWRCSRE"
BASE COUNT          663 a          791 c          725 g          681 t
ORIGIN      
        1 agatcatcgg gaacacgacc aacaagttga ttttttccat ctatgtgaga ccggagatca
       61 agacgatcga ggatctcaaa gggaaaaaga tcggcgtcac gcggtttggt tccgccactg
      121 atatcgccgc gcgcttcgct cttcgaaaac acaacctcga tccccaaaag gacgtgacca
      181 ttctgcaaat gggtgccatg acatcgatca tgggagggct gcagggcggt tccatcgatg
      241 ccgggctggt ttcgccgccg actctctttg cggggagaaa atgggcttta aagagctcgt
      301 cagcatgacg gatctggatc tcgcgtttcc caatccatca ctagtcgtgc agggcgggat
      361 cttgagaaaa aagccggaat cggtggatgg gttcatgcgc gcgtacgcga gaggtatcca
      421 gcgcgctcga accgacaaag agttcgcgct caaatccatt gcgaactaca ctaaaatcga
      481 agatccggca gtgctgcaaa aagcttatga tctttacgtc ggaaaagtct tggagaaggc
      541 gccctacatc aacatggtcg ggatgagaaa tgcgctcgcc gatttggcca aaacggttcc
      601 ggcggcaaag gatgtgaaac cggaacagtt cattgatatg cagttcctcg atcggctcga
      661 gcaaagcggt ttattgaaaa aactctaccc ctgagcacga agtggttaat ttgccaaaga
      721 ccgtgcttta caacgatttg attcaccccc tgctcttttg ggagatcccg aaaaaacgca
      781 cgagcagact ttggacgaat tggtgggtgc ggtttctacg gtttatggct gcagaaagga
      841 agatctgaga gcggcccggc ggcagtgaag gtgggccaag gcgcatgcgc agcttgcgta
      901 ttgggtacgc gagtggtccg cgatgacaac gactgaaatt cccatcggct gaccgcgacg
      961 catcgatgat gagccggctg tgcgccagtt ataaatcaaa tcaagacccg agacgcaaat
     1021 ggaattgcca aggtgattag aaaacaaatc acaattaggc ctgaccccaa cgcccgtctc
     1081 cggttcgtgg cagtgagagg ttacaagttc cctttcgatg ttgaacgctg ttcgtaataa
     1141 accagagtga aaccgggtga cgccgtgttg gcagcaatat tcgatttcac tgatccggac
     1201 agagtccccc ttctcctaac aaagtgacgc ctcgataaaa acacatacaa tggaacggca
     1261 aggttcgtct ggaattaata ccgcagccac cagttctgtt tgcgggaaat ttccggattt
     1321 accgaccgat gacgcattga tgcttatgac ggatcagaaa acgatctctt cattctccat
     1381 cgtcccgttt tatgctaagc gcgccagccg aaacactcaa cgcagatctc cacggtgtgc
     1441 tggcgcccaa cacgaaattg cgcagtaaaa tcgttcccgc cccggcacag cgcgccctga
     1501 aacctcgagc gaggacgctt acacgtacgg cgcgccggtg cgtatgaact gggcctgctg
     1561 gtcaaatcgg tccgatttgc tttcagaacg caaaatctct caaatcccat ctactcgcac
     1621 gttgctcttg acgatacgac cgaagtatcg cttttaatat ctcaagccca ttgcttgacc
     1681 gcaagtcgcg aacactcgct tcgatgccgg cgtttgcttg atccagccga aacgcttcgg
     1741 cgggaccgcc atctcgtaag gtggctctaa acggttcttg gaagactgca atgggctcgt
     1801 agcgcggatg gcacggaagc tcgcccagtt cctgttccag gccttgcact tgttccttca
     1861 acgtttgatt gtagacggcc agtttctcct cggtcagccg ttcaatctcg ccctcttcgc
     1921 gttgaatcca ttccatctca agcctaagca gcgtatgcat gtcgttgttg ctgtaggcca
     1981 cggtgagctc ctgcatgaga cgctcttttt gccttctgcg ttcaggatcc aattcgagat
     2041 ccggatgcag taccctcgca agctgcttgt acattgtcgc cacagtcttt ttgcgcagct
     2101 gctcggccag tttctcgcgt ttttctcttc aagttggcgc gccgttttac cgcgcggccg
     2161 aggcgtaaac gactgctgct gtttctcaat ctcctcctgc aaacgagtct tcatttcggc
     2221 ggccttgaca gccaatgcct cgtcgctcat taccggcttc atatcggaaa aatcgatatc
     2281 gatgccgaac tcgttaaaca tcgactccat ttcctgccgc gcttcttcaa actcctggcg
     2341 cgcaacctct tcgaagtcct ctccgcttac gcgcttaaat agcgcccgaa gatcatcgtc
     2401 ggacaacgaa ccttcgtcag cgacaatggc gtcgagctga tccgcaatca gatcgcatag
     2461 tgtgaaccgc gccttttttc ccttcaggcg cttgttacga agcgctccgc tcaaaaatag
     2521 agagcgcgac gcgccggctc gatgatctgc tcgcctatta cggggaacat cttcattccc
     2581 ggctgcagcg ccagacggag ttgcgtaaag ctctcgcacg cacgctcgcg actttcctag
     2641 aaatcctttt gattagacga ttaaatgtct gctgcgcttt ggtcagcggc cggccgcgcg
     2701 gcgcgaccaa cgtcagttca tcgaattttg cttccacacg gaacgcgccg tcattgaata
     2761 aggatatctg ctcgctcatt tgattatgtc accttgtttg ttcgtattgg ttggatcaaa
     2821 acttagaccg aatgcctaat ctctcgtgca agcgacgtca
//
LOCUS       DASULZ010000709         3566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_790646, whole genome shotgun sequence.
ACCESSION   DASULZ010000709 DASULZ010000000
VERSION     DASULZ010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790646"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            543..716
                     /locus_tag="VFY96_14705"
     CDS             543..716
                     /locus_tag="VFY96_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607773.1"
                     /translation="MRRLGSHPSTAKRLIRRCPMCKQAMGILIADPVKNVRPIHGFCM
                     RCGYGFRWAIITS"
     gene            1016..2254
                     /locus_tag="VFY96_14710"
     CDS             1016..2254
                     /locus_tag="VFY96_14710"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5607774.1"
                     /translation="MQEEVLQQVDRNRIVELACNLANIVSITGEEEEVAKYIGSELEQ
                     LGMQVEYQYVEEGRPNVVGMLKGTGGGATLMFNAHMDHFDNPQETVVDEDRIYGRGLV
                     NMKAAFPCYIEAVAAIQKAGVKLKGDVIIAGVVGEIEKAQIGRFRGKTYRGAGVGARY
                     LMDHGVMADMCIIGEPTGLQLQIGNSGLLWARVSVDGKATKFVLAACKVAQAIQDWER
                     EYQRKNPHKFMLPTVQIGAIEGGNPFKPGTRPTTEIFVIVKMLPGAVPLAIKRDIEQV
                     CERVRKREPDILDVRVDLYLTTPGYEISKSEPLVKVMERAHRAVFNEPVSYAKPIRYG
                     ITSDGSRIAQYGVPAITYGPGFGTHLVDPTETKAGEEWDSPSGIRRGVGIANMVNCTK
                     VYAAAALEICNTKKEEVAKR"
     gene            2373..3215
                     /locus_tag="VFY96_14715"
     CDS             2373..3215
                     /locus_tag="VFY96_14715"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607775.1"
                     /translation="MFRTYYALAIGKVKPDGVNMKVIELPDPPSKDQEEALIRGDVQA
                     ANLYLPNFLRRKLQGAPIIGLATEWKSTIKGNGVFVRADAPINTPQDLEGRLIATHQG
                     PHAIHQYLLRRVYGVDDTKLRWASYPQERLLDVLQRNEADAVVLLDHFFFRGEVAEGV
                     RCLYTDGEAWKKLHGFDEMIKHMVAAREDLLQRSPGIKETLLRAFRASFAYSERHLEE
                     MADAFISRYGGDKEALLASARYPKIEFTFTEKEQRLAEAEMELLVEVGQIARKAPLAS
                     LFSM"
     gene            3361..>3566
                     /locus_tag="VFY96_14720"
     CDS             3361..>3566
                     /locus_tag="VFY96_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607776.1"
                     /translation="MQLTHTATLIEEVKSFGKSLGIEPTPALSRTIQDSQPLSMLWLW
                     MQRAGTLALEGPIDVRLAIGFSTE"
BASE COUNT          812 a          959 c         1022 g          773 t
ORIGIN      
        1 ccgtgagacg ctccgctttg ctactgagtg ccgcctggat actatggaac cacaccacga
       61 atccggcgtc gtctcaggaa acttggacac cgttgtcgca gtttaagacc agatctgatt
      121 gtgtggaagc agcacggggc acagtccaga gacacgcctc caccccgcgc accatcgaaa
      181 gtagcaggct tgtttcacct aaacagcaac cgcagaagct gctcgtgtac ttgggcgacg
      241 acgctatttc tgtcataagc tacgaatgca atccggccac aaaaactcca gccgccatta
      301 atcgagctca tccgagttgc aaggattcgg attgtaaacg cgatccctcg gaacaaagcc
      361 gataatgcgc agtaggtcct tcgctctcac tcgtcggttc gcgtctttca cacgctgcca
      421 cgtgctgcgc gctcctgcta gccgcaattc gcccacaaac gttgccatct tttttcggac
      481 cgcgcagttt gttatcaaag atagagagag cgggctcgca aaccgggcgc tgttcttgct
      541 ctatgcgcag attaggctcc catccatcga cggcgaaacg gctcattcgt cgttgtccta
      601 tgtgcaaaca agccatgggc attctcattg ccgatccggt gaaaaatgtt cgaccgatcc
      661 acggcttctg catgagatgc ggctacggct ttcgctgggc gatcatcacg tcctgagctt
      721 agcctgaatt ccatgcatgg tttgaaacag ttcctgcgac aagtctccgg cacttcgagt
      781 gcggttgcgc agcagcgctc aacacgcagt tcgtccgtct cgggagttcg gcactagagc
      841 tgacggattc tcgatacgtc gctaacctcc ggtttttata gatccctatc gatccatttt
      901 ggcgaaccgg gtgagcacct gctgcgcatt gccgctgtcg cttcgttgtc aatccttacg
      961 gccgtatgtt agagaaaagg gctggtcttc attccagaga ggagctcttt tcaagatgca
     1021 agaggaagtg ctgcaacagg tggatcgcaa tcgaatcgtg gagctggcgt gcaatttggc
     1081 gaacatcgtc agcatcacgg gcgaggagga ggaagtcgcg aaatatatcg gcagtgagtt
     1141 ggaacagctc ggcatgcagg tggagtatca atacgtggaa gagggcaggc ccaacgtggt
     1201 gggaatgctt aaaggcacgg ggggtggcgc gaccttgatg ttcaacgcgc acatggatca
     1261 tttcgataat ccgcaggaga ccgtagtgga tgaagaccgg atttacggcc gcggtcttgt
     1321 caatatgaaa gctgcgtttc cctgctacat cgaggcggta gcggcgatcc aaaaagccgg
     1381 agtcaagctc aagggtgacg tcattatcgc aggtgtggtc ggcgagatcg agaaggcgca
     1441 gattggcaga tttagaggca agacttaccg cggcgccggc gttggcgctc gttatctgat
     1501 ggatcacgga gtgatggccg acatgtgcat catcggcgag cctaccggct tgcagctgca
     1561 gatcggcaat tccggcttgc tctgggctcg cgtgagcgtg gacggcaagg cgacgaaatt
     1621 tgttctggcc gcttgcaaag tcgcgcaggc gattcaggac tgggaaaggg agtatcagcg
     1681 taagaatccg cacaagttca tgttgccgac cgtacagatc ggcgccatcg agggcggcaa
     1741 cccgtttaag ccaggcacac gtccgaccac cgagattttt gtcattgtca aaatgctgcc
     1801 cggggcggtg ccgttggcga tcaaacgcga catcgaacag gtgtgcgagc gtgtacgcaa
     1861 acgcgagccg gacattctcg acgttcgggt cgatctctat ctcaccacac cgggatacga
     1921 aatttccaaa agcgagccgc tcgtcaaagt gatggaacgg gcgcaccgcg cagttttcaa
     1981 tgaaccggtt tcgtatgcca agccgatccg ctacggtatc accagcgatg gctcacgcat
     2041 cgcccagtac ggcgtaccgg cgatcaccta cggtcctgga ttcggcactc atctggtgga
     2101 tccgacggaa accaaagcgg gggaagagtg ggattcgccc agcggtattc gccgcggtgt
     2161 cggcatcgcc aacatggtca attgcaccaa agtttatgcc gcggcggcct tggaaatttg
     2221 caataccaaa aaggaagaag tggcaaaaag ataaattcta gattcgaagt tccgggtccg
     2281 cttcatagcc ggaccgttga acctcaaact ttgaacgaca cgctgaaatc aaaatggatc
     2341 tgaccttcgc gcattaccgg aatcgttttt gtatgtttcg gacctattat gccctcgcga
     2401 tcggaaaggt gaagccggac ggcgtcaata tgaaagtgat cgaattgccg gatccgccga
     2461 gcaaagacca agaggaagca ttgattcgcg gcgacgtgca ggcggcaaat ctttatttgc
     2521 cgaattttct tcggcgcaag ctgcaaggcg cgccgatcat cggtctcgcc accgaatgga
     2581 agtcgacgat caaaggtaat ggtgtgttcg tgcgcgcgga cgctccgatc aacacgcccc
     2641 aagatctcga aggccgcctg atcgccacgc accaggggcc gcacgcgatt catcaatatc
     2701 tgctgcgccg agtttatggc gtggacgata cgaagctacg ctgggcgtcc tatccacagg
     2761 aaagactttt ggacgtgctg cagaggaacg aggccgacgc cgtggtgttg ctcgatcatt
     2821 tcttctttcg cggcgaagtt gccgagggcg tacgctgcct ctacaccgac ggagaagcct
     2881 ggaaaaaatt gcacggcttc gatgaaatga tcaaacatat ggtggccgct cgtgaggact
     2941 tattgcagag atctccgggt atcaaagaaa cgttgctcag agcgtttcgc gcatccttcg
     3001 catacagcga gaggcatctc gaagagatgg ccgacgcttt tatctcccgc tacggcggcg
     3061 acaaagaggc cttgctggct tcggcgcgtt atccgaaaat tgaattcacc ttcaccgaaa
     3121 aagagcagcg actcgccgaa gcggaaatgg agttgctcgt ggaagtgggg cagatcgcgc
     3181 gtaaggcgcc gcttgcgtct ctcttctcga tgtgagctgc ttgggtgcag ttaaggctct
     3241 tcagttcacc atttcaccgt ggtgtcggcc aaacactgga tcggattttt cgtcgcgctc
     3301 gccgtcggcc ttgtggtttc gtcttgcgct aggctggtaa ctcctcgcat tgaagacagg
     3361 gtgcagttga ctcataccgc gacgcttatt gaggaagtaa aaagcttcgg caaatccctc
     3421 ggtatcgagc cgactcccgc gctcagccga accatccaag atagccagcc attatcgatg
     3481 ttatggctct ggatgcaacg cgccggaacc ctggcgctcg aaggccccat cgacgtacgc
     3541 ttggcgatcg gttttagcac cgagag
//
LOCUS       DASULZ010000710         4056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_588880, whole genome shotgun sequence.
ACCESSION   DASULZ010000710 DASULZ010000000
VERSION     DASULZ010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588880"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VFY96_14725"
     CDS             <1..558
                     /locus_tag="VFY96_14725"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEX5607777.1"
                     /translation="GSATILVEAALLALDIAPGLGRQFAFEKFKNFDAPRWRELRRIT
                     VARQKPKKLLAIYGSDIDPAALEAARANLAGAGVAECVALKCAVVLDITPPTREGIIV
                     SNPPYGVRLGEQQRLADFYPRLGDLLKKHFTGWRVYFLSADMRLPKLIHLAVSRRVPL
                     FNGALECRLFEYKMVAGRMNRKREE"
     gene            complement(638..1933)
                     /locus_tag="VFY96_14730"
     CDS             complement(638..1933)
                     /locus_tag="VFY96_14730"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016862117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEX5607778.1"
                     /translation="MKRVLAIILGGGAGTRLYPLTKLRAKPAVPLGAKYRLIDIPISN
                     CINSEIERIYVLTQYNSASLNRHIARTYHLAGAGFSQGFVEVLAAQQTPESPSWFEGT
                     ADAVRKYLWLFAECDVDQYLILSGDHLYRMNYADFVARHRETKADITIGVVPMDEARA
                     SDFGLMKIDDTGRVRSFSEKPKGEALKEMRVDTTVLGLTAEEAARAPYIASMGIYIFE
                     KQVVHEVLDRDRDRADFGREILPDSLRDYNVQAYLFNEYWEDIGTIEAFYNANLALTE
                     QPDPPFSFYDETAPIYTRSRYLPPTKLLDSHVTQSMLGEGCILKECTVTRSVIGIRSR
                     IETGCVIDNALLMGCDFYESPEQRLQALESGKTPLGVGANSTIRRAIVDKNARIGRNV
                     QIINRDHIVEGNRENLGFVIQNGIIVVIKNATIPDEMVI"
     gene            complement(2134..2334)
                     /locus_tag="VFY96_14735"
     CDS             complement(2134..2334)
                     /locus_tag="VFY96_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607779.1"
                     /translation="MNLDKRADAPLLTMCRIFKLLVIALFSLTTFPAPAGSYDVICIG
                     IISTTFGYAPVFVAKEKGLFKR"
     gene            2426..>4056
                     /gene="ppk1"
                     /locus_tag="VFY96_14740"
     CDS             2426..>4056
                     /gene="ppk1"
                     /locus_tag="VFY96_14740"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEX5607780.1"
                     /translation="MDGDSELFINRDLSWLEFNRRVLEEAKSAAVPLLERVKFLAIFS
                     SNLDEFFMVRVAGLKRQLASDPMSNSNGTAPEAILRAISRRVHELTDEQHRVFLSTVL
                     PPLTREGVHLVRAEELTREQEQFLEEFFQRTLYPIVTPLAIDPGHPFPYLTNRSLSLV
                     VSLRPVAPSPLPHTELSIVHIPGQVVPRFIQLPAKEGQYAFILLEHVLRRYLPLFYHG
                     FEIVSTHAVRVTRDAEFGLVRRRDQDLLMSIEQGIRERRMGDAVRLQYDPDLPSEVLR
                     QLIEELELTQDDLYAGQGFTAFTDLFQLYAAANIPRLRDKPQLPLPVPCFERGPDIWN
                     SIRSNDVLVFHPYQSFDAVTHFVEEAAKDPKVLAIKMTLYRVSANSPIATALARAAEA
                     GKEVSVLVELQARFDEEANITWARALEEVGAHVVYGLLGYKTHCKVCLVVRQEVDGIR
                     RYCHLATGNYNVRTSSVYSDFGLFTCRPTFGEDLTELFNLLTGYTRPQRFHHLVLAPT
                     GLREHFIDLIRNEAEQARAGKPARIIAKVNSLIDDA"
BASE COUNT          878 a         1072 c         1154 g          952 t
ORIGIN      
        1 ggcagtgcca cgattctcgt cgaagcggcg ctgctcgccc tcgacatcgc gccgggatta
       61 ggacggcagt ttgctttcga aaaatttaaa aattttgacg ccccgcggtg gcgcgagcta
      121 cgccggataa ccgtggcgcg ccagaaaccc aaaaagcttc tggccattta tggaagcgat
      181 atcgatcccg cggccttgga ggcggctcgc gccaatctcg ccggtgccgg ggtcgcagaa
      241 tgcgtcgctc tgaagtgcgc tgttgttctg gacattacgc caccgacgcg agaaggcatc
      301 atcgtcagca atccacctta tggtgtgcgt ttaggggagc agcagagatt ggctgacttt
      361 tatcctaggc tgggcgactt gttaaaaaaa catttcacgg gttggcgggt gtatttcctg
      421 agcgccgaca tgcggcttcc caaactcatc cacctggcag tatctagacg cgtacccctg
      481 tttaacggcg ccctcgaatg ccgtctgttt gagtacaaaa tggttgcggg gcggatgaat
      541 cggaaacgtg aggagtaagg cgtgaggggt gagaagtaag aaaaagtgag gcgtcagggg
      601 tgcagacggg cttaccctct ccacttactc ctgacgttta gattaccatt tcatcgggaa
      661 tggttgcgtt cttgatcacg acgatgatgc cgttttgtat gacgaagcct aggttttctc
      721 ggttgccctc cacaatatga tctcgattga tgatctgcac attgcgaccg atgcgggcat
      781 ttttgtcgac gatggcgcgc cggattgttg aattcgcgcc gacgcccagg ggcgtttttc
      841 cgctctccag cgcctgcagg cgttgttccg gcgattcata gaaatcacag cccatgagca
      901 atgcgttatc gatcacgcat cccgtttcga tgcgcgagcg aatcccgatg acagagcgcg
      961 ttacggtgca ttcttttagg atacacccct cgcccagcat cgattgggtc acgtggctgt
     1021 cgaggagctt ggtcggcggc agatagcgcg agcgagtata aatcggcgct gtttcgtcat
     1081 aaaagctgaa cgggggatcg ggttgctcgg taagcgcaag attagcattg taaaacgcct
     1141 cgatggttcc gatatcttcc cagtattcgt tgaatagata agcctgtaca ttgtagtcgc
     1201 gcaaagagtc gggaaggatt tctctcccga aatcggctcg gtcacggtcg cgatccaata
     1261 cttcatgcac cacttgtttc tcgaaaatat aaatgcccat tgaagcgata tagggagcgc
     1321 gcgctgcttc ttcggccgtt aaaccgagaa ccgtggtatc cacccgcatc tctttaagcg
     1381 cctcgccttt cggtttttcg ctgaaagagc gcacccgtcc ggtgtcatcg atcttcatca
     1441 aaccgaaatc cgacgcccgc gcttcgtcca tcggcacgac cccgatcgtt atgtccgctt
     1501 tggtttcgcg atggcgcgcg acgaaatcag catagttcat acggtagagg tgatcgccgg
     1561 acaaaatcag atactgatcg acgtcgcatt cggcaaagag ccagagatat ttgcgcaccg
     1621 cgtctgcggt gccttcgaac cagctcgggc tttccggcgt ctgctgcgcg gccaggacct
     1681 ctacgaaccc ctgggaaaaa cctgccccgg ccaggtgata agttcgggcg atatgccggt
     1741 tcagcgaagc ggagttgtat tgagtgagta cgtaaattct ttcaatttcg gagttgatgc
     1801 agttgctgat cgggatgtcg attagacgat atttggcgcc caggggcacc gctggcttgg
     1861 ctcggagttt ggtcaacgga tagagccggg ttcccgcgcc gcctcccaaa atgatcgcca
     1921 atacacgttt cacgtacaga cctcatttca aagattgttg cattgcgcgg gccttgccgt
     1981 ggaaagaaca tacgcaacca gggacggcca agtcaaaccg gaaaaatcaa gatcggacgc
     2041 gctcttgttg caaaccgccg cgatggcggg tcagtgaaaa cttctaccgt cgcgcaaacg
     2101 cattgacctg acaacgacct cgggatcaag accttagcgt ttgaaaagtc ccttctcttt
     2161 ggcgacgaat accggcgcat agccgaaggt ggtgctgatg atgccaatgc agataacatc
     2221 atacgaaccg gccggcgccg ggaacgtggt cagcgaaaac aatgcgataa caagcagttt
     2281 gaaaattcgg cacatggtga gaagcggggc atctgcgcgc ttgtcaagat tcacgataat
     2341 gatcgccctg ccaagatacc tggaatttga gccgaatttg gttctccgtt gttcggacca
     2401 aggaaagagt taaactgaga tgattatgga tggcgattcg gagttattta tcaatcgcga
     2461 tctttcctgg ctcgagttta accggcgcgt tctggaggaa gccaagtctg ccgcagtgcc
     2521 gcttctcgag cgggtgaaat ttctcgcgat tttcagttcc aatctggatg aattttttat
     2581 ggttcgggtt gcgggtctga agcgccaact cgcgagcgac ccgatgagca attcgaatgg
     2641 aacggcgccg gaagcgattt tacgggcgat ctcgcgccga gtgcatgagt tgacggacga
     2701 acaacatcgc gtctttctca gcaccgtcct tccgccgctg acacgggaag gggttcatct
     2761 ggttcgcgcc gaagagctaa cccgtgagca ggagcaattt ttagaggaat tctttcagcg
     2821 cacgctatat cccatagtca cgcctttggc gatcgatccg gggcatccgt ttccgtatct
     2881 cacaaatcgg tcgctatctt tggtcgtgtc tttgcggccg gtcgcaccct cgcctttgcc
     2941 gcataccgag ctctcgatcg tgcatattcc aggtcaggtg gtgcctcgat tcatccagct
     3001 gcccgccaag gaaggacagt acgcctttat tttgctcgag catgtgctgc gacgatactt
     3061 gccgcttttt taccacggtt ttgagattgt ctcgacacat gcggtgcggg tcactcgcga
     3121 tgctgagttc ggtttggtgc gtcggcgcga tcaggatctg ctcatgagca ttgagcaggg
     3181 aatccgagag cggcgtatgg gcgatgccgt tcgccttcag tacgatcccg atcttcccag
     3241 cgaagttttg cggcagctga tcgaagaact tgaattgacc caagatgatt tgtacgccgg
     3301 ccaaggcttt acggcgttca ctgatctctt tcagctctat gccgcggcga acattccacg
     3361 gctcagggac aaaccccagc tgccattgcc ggtgccgtgc ttcgaacggg gtccggacat
     3421 ttggaacagt attcgaagca atgatgtttt ggtttttcat ccgtaccagt ccttcgatgc
     3481 ggtaactcat ttcgtcgaag aagcggccaa agatcccaaa gttttggcaa tcaagatgac
     3541 gctctatcga gtgagcgcca attcgccgat cgcaacggcc ctggcgcgcg ctgccgaagc
     3601 gggtaaagaa gtgtcggtgt tggtggagct gcaggcgcgc ttcgatgaag aggcaaatat
     3661 cacctgggcg agagcgctcg aggaggtggg agcgcatgtg gtttacggcc tgttgggcta
     3721 caaaacgcac tgcaaagttt gcctggtggt gcgacaggaa gtcgatggca tccggcgcta
     3781 ttgtcatctc gcgaccggca attacaacgt gcgcacgagc agcgtttaca gcgactttgg
     3841 actgttcact tgccggccaa ctttcggcga ggatctcacg gagttattca atctgctgac
     3901 gggctacacc cggccccaac gattccatca cctggtcctg gcgcccactg ggttacgcga
     3961 gcatttcatt gatttgattc gcaacgaggc ggagcaggcg cgcgccggca aaccagcgcg
     4021 cattatcgcc aaggtgaaca gcctgattga cgatgc
//
LOCUS       DASULZ010000711         2035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_384539, whole genome shotgun sequence.
ACCESSION   DASULZ010000711 DASULZ010000000
VERSION     DASULZ010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384539"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..873
                     /locus_tag="VFY96_14745"
     CDS             4..873
                     /locus_tag="VFY96_14745"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX5607781.1"
                     /translation="MAEQTLSGLKLFNRGKVRDIYDLGDKLLLVASDRISAFDVILPT
                     LIPDKGKILTRLSEFWFGIMKDIVPHHLITTRVEEFPAACHPHKAELEGRSMLVKKST
                     PAPVECIVRGYLVGSGWKDYQQTGAVCGVALPQGLVEASRLDQPIFTPSTKAPVGAHD
                     VNITFEEMAVKIGRKRAERMRDVTIAIYSRARDLADKKGILIADTKLEFGIEGDDIML
                     IDEVLTPDSSRFWPADGYRPGKTPDSFDKQFVRDYLLNLPWDMKSPPPALPREIVKKT
                     QEKYAEALRRLTS"
     gene            complement(939..1457)
                     /locus_tag="VFY96_14750"
     CDS             complement(939..1457)
                     /locus_tag="VFY96_14750"
                     /inference="COORDINATES: protein motif:HMM:NF013624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607782.1"
                     /translation="MSKRILVYGFGPYGAFPYNITARILKTLPRQPGLTKRVFPVRFN
                     RQQFVDALRRAKPDIILGLGQSSRQGIEIESRGVNHRRPDRSAKRRAIFAREPKQLAT
                     TFKIKPGRWSRISRNAGDYVCNYSIYVLLREIKQQKLAARLAFIHIPHDYNLKKARKF
                     IRHLLRQCETEK"
     gene            complement(1548..>2035)
                     /locus_tag="VFY96_14755"
     CDS             complement(1548..>2035)
                     /locus_tag="VFY96_14755"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEX5607783.1"
                     /translation="YGCAMLCYVTPKEHLGLPNKEDVKAGVITYKLAAHAADLAKGHP
                     GAQFRDNALSKARFEFRWEDQFNLSLDPDTAREFHDETLPAQGAKLAHFCSMCGPHFC
                     SMKITQDVREYAAQKGLEEQAALAAGMQEKAEEFKKTGAKIYQEVGSSASAENRAAAK
                     E"
BASE COUNT          444 a          539 c          558 g          494 t
ORIGIN      
        1 atcatggctg aacaaacttt atccggttta aaacttttta atcgcggcaa agtgcgcgac
       61 atttacgatt tgggcgataa gctcctgctg gtcgccagcg atcggatttc ggcttttgac
      121 gttatcttgc caaccttgat cccggataag ggaaaaatcc tcacccgatt gtcggaattc
      181 tggtttggca tcatgaagga tatcgtcccg catcatctga tcacaacgcg agtggaagag
      241 tttcctgcgg catgccaccc gcacaaagcc gagctggaag gccgctcgat gttggtgaag
      301 aagagtacac ccgcaccggt cgaatgcatc gtgcgcggct atctcgtggg gtccggctgg
      361 aaagattatc aacagactgg cgcggtttgc ggcgtcgcgc tgccgcaagg attggtcgag
      421 gcatccaggc tcgatcagcc gatcttcacg ccgtccacca aggcgcccgt tggcgctcat
      481 gatgtgaaca tcacatttga agaaatggcc gttaagatcg gcaggaagcg tgccgagaga
      541 atgcgcgatg tgaccattgc gatttattcg cgcgcccggg atttagctga taaaaaaggc
      601 attctcatcg ccgataccaa gcttgagttc ggcatcgaag gcgatgacat tatgctgatt
      661 gatgaagtgc tgacccctga ctcgtctcga ttctggccag ccgatggtta cagacccgga
      721 aaaacgccgg atagctttga taaacaattt gtccgtgact atctgctcaa tctgccctgg
      781 gacatgaaat caccgcctcc cgcgctgccg cgggaaatcg ttaaaaagac gcaagaaaaa
      841 tatgccgagg ccttgcggcg attgaccagc taacgctcga ctctctccaa cgatcttatg
      901 ggccgggcag aatgctttct cccgacctag acgccacttc acttttctgt ctcgcactgc
      961 cgcagaaggt gtcggataaa cttgcgcgcc tttttcaggt tataatcgtg cggaatgtga
     1021 atgaatgcca gtcgggcagc taatttctgc tgtttgattt cacgcagaag aacatagatc
     1081 gaataattgc agacgtagtc gccggcgttt ctcgaaatcc tcgaccatcg acctggtttg
     1141 attttgaacg tcgtagctaa ttgcttcggt tccctcgcaa agatcgctcg gcgtttggct
     1201 gatctatcgg gccttcgatg gttgactccc ctgctttcaa tttcgatccc ctgccgcgaa
     1261 ctttgtccga gccccagaat gatatccggt ttggcgcgcc tcaatgcatc gacgaattgt
     1321 tggcggttaa aacggactgg aaaaacgcgc ttggtcaatc cgggctgccg cggcagggtt
     1381 ttaaggatgc gggcagtgat gttatatgga aacgccccgt atggaccaaa accgtaaacc
     1441 aagatgcgct tgctcatcga tccgaacgac gtcgatgtgt ggggtgctgg gccgaaaaaa
     1501 aactggccgg cattgcgccg gccagttttc tacagaaatg aaacgcgcta ctcttttgcc
     1561 gccgccctat tttctgcaga agctgaagag ccgacctctt gataaatttt tgctccggtt
     1621 tttttgaact cttcggcttt ctcttgcatg ccggcagcga gcgccgcctg ctcctcgaga
     1681 cctttttggg cagcatactc gcgcacatct tgggtaatct tcatcgagca gaagtgcggt
     1741 ccgcacatcg agcagaaatg cgccagcttg gctccctgcg cgggcagcgt ttcatcatgg
     1801 aattcccgcg ccgtgtccgg atccaggctc aaattgaatt gatcttccca gcgaaactcg
     1861 aaccgcgctt tcgataaagc gttgtcgcgg aactgagcgc cgggatgacc tttggcgagg
     1921 tcggcggcgt gggcggcgag tttgtaggtg ataacgccgg ctttgacgtc ttccttgttg
     1981 gggaggccga ggtgttcctt gggcgtgacg tagcagagca tggcgcagcc gtacc
//
LOCUS       DASULZ010000712         2851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_182603, whole genome shotgun sequence.
ACCESSION   DASULZ010000712 DASULZ010000000
VERSION     DASULZ010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182603"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="VFY96_14760"
     CDS             complement(<1..1011)
                     /locus_tag="VFY96_14760"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HEX5607784.1"
                     /translation="MSKPMPKNGAADAGEAKLTISAAEMGARQREISVSEFFTKNRHL
                     LGFDNPRKALLTCVKEAVDNALDACEEAGILPEVVVKLEVASNGDPAPPPSQANRFRV
                     TVIDYGPGIVRQQIPRIFAKLLYGSKFHRLRMSRGQQGIGISAAGMYAQLTTGKPVQI
                     ISRTGARTPAHYFEVQIDTKKNEPRIFEQKQIEWENHRGTQVTLEIEGRYQKGRASVD
                     EYIEQTAIANPHVKVVYHTPEGEIKEYPRTIEELPPQPREIKPHPYGIEFGILLRMLH
                     DTKSQSLAGFLSSDFSRVSPNIAQEICKVAKLSPDTRPRKVHGAPAEALYKTIQSTKI
                     MAP"
     gene            1315..2598
                     /locus_tag="VFY96_14765"
     CDS             1315..2598
                     /locus_tag="VFY96_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014688461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5607785.1"
                     /translation="MPSTLSTVRSQNSPITARKIAFAALQHRHFRGFFISTMLAMMAD
                     NIEHVISYWVLFQKFQSPVLAGFAVISHWTPFLFLSVYFGGLADRYDCRRLIQIAQVM
                     YMFVSAAWAFFFFTGSIEVWHACVLLVIHGVAGVFWTPAEQLIIHDIVGPEDLPSAVR
                     LNATSRQLGILFGPAVGGGLLLWLGPSAGLVVNALIYLPFTIWLAIVPYTGHTREGAV
                     PRRALRWRDAFDIMREISDNRPIVTMIVLGGAASLFVGNAFQANMPEFAHDLGTGKTD
                     FAYSALLAANAAGSVFGGFVLDGKGWLPPSAKSAIACAILWCLTMTAFAFSQSYLLSL
                     VLLFVAGALNLSFYSIAQTIVQLEAPLALRGRLIGLFSMSAYGLRAFSGFTVGVVGGL
                     IGIHWSLALSSMILLAVTMALLTFTSHGQRAEELG"
BASE COUNT          490 a          791 c          822 g          748 t
ORIGIN      
        1 aggcgccatg atttttgtcg attgaatcgt cttgtagagc gcttcggccg gagcgccgtg
       61 cacctttcgc ggacgggtat cgggcgagag cttggcaact ttgcagatct cctgcgcgat
      121 attcggcgaa acccggctga aatcgctcga caggaaacct gccagagatt ggcttttggt
      181 gtcgtgcagc atgcgcaaca atattccgaa ttcgataccg tacgggtgcg gtttgatttc
      241 acgcggttgc ggcggcagct cttctatcgt ccggggatac tctttgatct cgccttcggg
      301 cgtatgatag acgaccttca cgtgcggatt ggcgatggcg gtctgctcga tatattcatc
      361 caccgacgct cggcccttct gatatcgacc ttcgatctcc agcgtgacct gagtgccccg
      421 gtgattttcc cattcgatct gcttttgttc gaagatccgc ggctcgtttt tcttggtgtc
      481 gatttgcact tcgaagtagt gcgccggcgt tcgtgcgccg gtgcgcgaga tgatctgcac
      541 cggtttaccg gtggttaatt gcgcgtacat gcccgccgcc gagatgccaa tgccctgttg
      601 gccgcggctc atccgcagcc gatgaaactt ggacccgtat aaaagcttgg cgaagattcg
      661 cgggatctgc tggcgcacga tgcccgggcc gtaatcgatc acggtcacac gaaaccggtt
      721 cgcttgactg ggcggtgggg caggatcgcc gttcgacgca acttccaact tgaccaccac
      781 ctccggcaga atccccgctt cttcgcaggc atcgagagcg ttgtccaccg cttccttgac
      841 acaagtcagt agggccttgc gcgggttgtc gaaaccgagc aaatggcggt tcttggtgaa
      901 aaattcggaa acggaaattt ctcgctggcg cgctcccatc tccgctgcgg agatggtcag
      961 tttggcttcg ccggcgtcgg cggcgccatt tttcggcatc ggtttagaca ttgttgcggc
     1021 tattctccat tcgtgattgc caattgacac tgcggaatat tggagtcgaa aagctcacga
     1081 ctctaagttc caggtgtcgc gcagccaatt tgttattgga atgcgccgcg aaatgcgatt
     1141 ggttctgcgg cggcagatca gatgtttcct cggcgtttgc cgtgcgatgg ccggattata
     1201 ccagagtcga aatttttttg aactctttca tcgggtaatt ttatggatcg gcatgtttgc
     1261 atcggcgagc gagattcgtt atctcaaccg tagatcattc aacaggagaa ctgtttgcct
     1321 tcgaccctca gcacggtacg cagtcaaaat tccccgatca ccgctcgtaa aatcgccttc
     1381 gctgccctgc aacaccgcca cttccgtggt tttttcattt ccacgatgct ggcgatgatg
     1441 gcggacaata tcgagcacgt gatcagctat tgggtgcttt tccaaaaatt tcagtcaccg
     1501 gtgcttgccg ggttcgcggt gatcagccac tggacgccat ttttgttttt gtctgtctac
     1561 ttcggcggct tggctgaccg gtacgactgc cgacggctga tccagatcgc ccaggtgatg
     1621 tatatgtttg tttccgccgc ttgggcgttt ttctttttca cgggcagcat cgaagtgtgg
     1681 catgcctgcg ttctgctggt catccacggc gttgccgggg tgttctggac gcctgcggaa
     1741 cagttgatca ttcacgacat cgtcggtccg gaggatctgc cgagcgcggt gcgcttgaac
     1801 gcgaccagca gacaattggg tattcttttc gggccggcag tcggcggcgg cctattgctg
     1861 tggctcggcc cgtccgcagg attggtggtc aacgctttga tctatctgcc gttcacgatc
     1921 tggctcgcga tcgttcctta taccggccat acacgcgaag gcgcggtgcc gcgccgcgcg
     1981 ctccgttggc gcgatgcctt cgatatcatg cgggagatct cggacaatcg tccaatcgtc
     2041 acgatgattg tgcttggcgg cgcagcatcg ctgttcgtcg gtaatgcgtt tcaagccaat
     2101 atgcccgagt tcgctcacga cctcggcacc ggcaaaacgg acttcgccta cagcgctctc
     2161 cttgctgcca atgccgccgg ctcggtcttc ggcggctttg tcctcgacgg aaaggggtgg
     2221 ttgccgccgt cggctaaatc ggcgatcgcg tgcgcgatac tttggtgttt gacgatgacc
     2281 gcttttgcgt tttcacaaag ctatctgctg tcactggtgc ttttgttcgt cgccggcgcg
     2341 ctaaaccttt cattctattc gatcgctcag accatcgtgc aattggaggc cccgctggcg
     2401 ttgcgcggtc ggctgatcgg gcttttttcg atgtccgctt atggattgcg ggcgttcagc
     2461 ggcttcaccg tgggtgtcgt cggcggcttg atcggcattc attggtcgct tgcgctaagc
     2521 tcgatgattc tgctggcggt gaccatggcc ctcttgacct tcacgtcaca tggccagcgc
     2581 gccgaagagc tgggctgaaa atcctcgctg tcggtcgaac ttattactcc cactgccgaa
     2641 ggatctcgcg gcgctagatt cgcaactgtc gttctgagcg gaaggcgaag aatctcgcct
     2701 cgtaatccgg ccggtaagag agggagatag atgcttcgtt tcactcagca tgacagtggg
     2761 ttgtggatgg cgcattcccg caaggttccc cggaagatcc ctcgctttgc tcgggatgac
     2821 tcattcccag ttttttttgg agagatctct c
//
LOCUS       DASULZ010000713         3837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1401541, whole genome shotgun sequence.
ACCESSION   DASULZ010000713 DASULZ010000000
VERSION     DASULZ010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401541"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VFY96_14770"
     CDS             <1..314
                     /locus_tag="VFY96_14770"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX5607786.1"
                     /translation="CPSCGRADVNLVALAKAVEEKLLPYSNLDIHVAVMGCEVNGPGE
                     ARAADLGVAGGKGIGLIFKKGEVIRKVPEAQIVDALMEEVEKFAAERKAEQSAAAAAT
                     A"
     gene            606..2333
                     /locus_tag="VFY96_14775"
     CDS             606..2333
                     /locus_tag="VFY96_14775"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX5607787.1"
                     /translation="MRWSKTLIPTLKEDPADAEVISHKLLVRAGFIRQISRGVYDYLP
                     LALRVIRKIEQIVREEMNRAGAQELLMPISSPAELWQESGRWEKYGKELIRFKDRNDR
                     DFCLGPTHEEIVTDLVRRSVRSYRELPFNLYQIQTKFRDEPRPRFGLMRGREFIMKDA
                     YSFHPDVADCRREYENMFQTYKRIFSRCGLRYRPVEADTGAIGGSLSHEFQVLADSGE
                     DAIVSCNTCEYAANIEKAEIRTTRPAGRDISENEPKLEKVSTPGKKTVAEVSQFLKIP
                     PERFIKTLVYQTDGGELIAVLVRGDYELNELKLKTVLGMQEISLADEAAVASATGLVP
                     GFLGPIGLKLHTIADSSVHGMRAAVVGANEANAHFIHVDQERDFTPSAFADLRLAVAG
                     DPCPRCEAGILEAHRGIEVGQVFYLGTKYSVAMGATYLDAEGRERPIEMGCYGIGVSR
                     LVAAAIEQNHDANGIIWPYSIAPFQVLLLPINYKDSSIREAADALYANLQGTGVDVLL
                     DDRDERPGVKFKDADLVGIPLRLTIGPKTLDKGRIELRRRREGQTEEVALGEAIKKIN
                     SIISQPMQG"
     gene            2335..3090
                     /gene="pyrF"
                     /locus_tag="VFY96_14780"
     CDS             2335..3090
                     /gene="pyrF"
                     /locus_tag="VFY96_14780"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX5607788.1"
                     /translation="MASKLELVRRRGSMRERLIVALDFDDLELATELVQSLAQEVGMF
                     KIGKQLFTHAGPQAVRLIQDLGGEIFLDLKFHDIPNTVAKAAIEATRLGVKMFNVHAS
                     GSLEMMRLTVKEVERVSRQQKLRRPIMLGVTVLTSLGQEDLLRLGVEHKIADQVVRLA
                     LLTKEAGMDGVVASPHEVADIRQACGQRFVIVTPGIRPADSQRNDQQRVMTPADAVRA
                     GVDYIVVGRPILEAQNPVMAARGIVAEMEDRLK"
     gene            complement(3145..>3837)
                     /gene="thiD"
                     /locus_tag="VFY96_14785"
     CDS             complement(3145..>3837)
                     /gene="thiD"
                     /locus_tag="VFY96_14785"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX5607789.1"
                     /translation="AITASTAQNTIGVTQVLALKPEIVAGQIDAVMEDIGAHAVKTGM
                     LANASIIEAVAIKIQQHRFKNVVIDPVMVATSGDLLIKKNAVATLRSLLVPLATVLTP
                     NLPEAEQLTGMNLRTPNDVEEAARRIIAMGARTVVIKGGHRKGAALDLFFDGKSFRVL
                     RAARIHSRNTHGTGCTFSAAIAAYLARGEALERAVSLAKEYITGAIRAGFVIGAGHSP
                     VHHFYRLWSRKK"
BASE COUNT          852 a         1042 c         1121 g          822 t
ORIGIN      
        1 cctgcccatc gtgcggccgg gccgatgtca atctcgtggc gctggcgaaa gcggtcgaag
       61 aaaagctgct tccctacagt aacctggata ttcatgtcgc ggtgatgggt tgtgaagtga
      121 atgggccggg cgaggcgcgg gcggccgatt tgggcgtggc gggtggtaag gggataggtt
      181 tgatcttcaa gaagggcgaa gtcattcgca aggttcctga agcgcaaatt gtcgatgctt
      241 taatggagga agtcgaaaaa ttcgctgcgg aaagaaaagc ggaacagagc gcggccgccg
      301 cagcgacggc ctaattttct ctctccaaaa gattcctaat ctgcgtcctg cgtcgcaaac
      361 cgaattgtgg tgaaaaggct taaccacgaa gagcacgaac gctgggaatt ttctccgtca
      421 gaaatcttcg tgtccttttc gcgctcgttc gaaaatagga ctggagaaag atctctcact
      481 ttcgttcgaa atgacagaag tgtcattccg agccggcggc gaggaatctt tccctcggca
      541 ttttcatctt ctggggcgag cgtaagctca tgaattgctt cgcggtcata cgttatcact
      601 gagttatgcg ctggagcaaa acgctcatcc ctacgctcaa ggaagatccg gcggacgctg
      661 aggtgatcag ccataagcta cttgtgcgcg cgggctttat ccgccaaatc agccgcggcg
      721 tctatgatta tttgccgctg gcgctccgag tcatccgtaa aatcgaacag atcgtccgcg
      781 aggaaatgaa ccgagcgggt gcgcaggaac tcttgatgcc gatctcgtca cctgccgagc
      841 tttggcagga gagcgggcgc tgggagaagt acggcaaaga gctcattcgt ttcaaagacc
      901 gcaacgatcg cgatttctgc ctcggtccga cgcacgaaga aattgttacc gatctggtgc
      961 gccgctcggt gcggtcctac agggaattgc cgttcaatct ctaccagatc caaaccaagt
     1021 ttcgcgacga accgcggccg cgtttcggct tgatgcgtgg ccgtgagttc atcatgaaag
     1081 acgcttacag ttttcatccg gacgtcgcag actgccgccg cgaatacgaa aacatgtttc
     1141 agacatataa gcggattttt agccgttgtg gtttgcgcta ccggccggtc gaagcggata
     1201 cgggtgcaat cggcggcagc ctctcgcacg agtttcaggt gctcgccgac tcgggcgaag
     1261 atgcaatcgt cagttgcaat acctgcgaat atgccgccaa tatcgaaaag gccgaaatca
     1321 gaacgacgcg gccggcggga cgcgacatca gcgaaaacga gcccaagttg gaaaaagttt
     1381 cgactccggg aaagaagact gttgcggagg tgtcgcaatt tctcaaaatt ccgcctgaac
     1441 ggtttatcaa aacgctcgtt taccaaaccg acggcggaga actcatcgct gtactggtgc
     1501 gcggcgatta tgagctcaat gagctcaagt tgaaaacagt attggggatg caggaaatca
     1561 gtcttgccga tgaagccgca gtggcgtcgg cgacgggact ggtcccggga tttctcggcc
     1621 ccattggtct gaagctccac acgatagcgg actcatcggt ccatggcatg cgcgcggcag
     1681 tggtcggcgc caatgaggcc aatgcccatt tcattcacgt cgatcaggaa cgagatttca
     1741 cgccatcggc tttcgcggat ctaagactag ccgtagccgg cgatccatgc ccgcgctgcg
     1801 aggcgggcat ccttgaagcg catcgtggca tcgaggtcgg tcaagttttt tatctcggca
     1861 ccaaatacag cgtcgcgatg ggcgcaactt atctcgatgc cgaagggcgc gagcggccca
     1921 tcgagatggg ttgctacggc atcggcgtaa gccggctggt ggccgctgcc atcgagcaaa
     1981 accatgacgc gaatggcatc atctggccat actcgattgc gccgtttcaa gttctgctct
     2041 tgccaatcaa ttacaaagac agcagtatcc gcgaagctgc cgacgcgctc tatgccaatt
     2101 tgcagggaac cggcgtcgac gttctgctcg acgaccggga tgagcgacca ggcgtgaaat
     2161 ttaaagacgc cgacctggtg ggaattccat tgcgcctgac gattgggccc aaaaccttgg
     2221 acaagggccg aatcgagctg cggcggcgac gcgaaggcca aaccgaggag gtcgctctcg
     2281 gcgaagcgat aaaaaagatc aactcgatca tttcccaacc tatgcagggc tagcatggca
     2341 tcgaagctcg agttggttcg tcgacgtgga tccatgcgcg agcgtttgat tgtcgctctc
     2401 gacttcgatg acctcgaact ggccacggag ttggtgcaat cgctcgccca ggaagtcggc
     2461 atgttcaaga tcggcaaaca actattcacg catgccggtc cgcaggcggt gcgtctgatt
     2521 caagacctcg gcggtgaaat ttttctcgat ttaaaatttc acgacattcc gaataccgtc
     2581 gccaaagcgg cgattgaagc gacccgcctc ggcgtgaaga tgttcaatgt gcacgcttcg
     2641 ggaagcctcg aaatgatgcg gctgacggta aaggaagtgg agcgtgtgag ccgacagcaa
     2701 aagcttcgcc ggccgattat gctcggggtc accgtcctga cgagcctcgg acaagaggat
     2761 ttgctgcggc ttggagtcga acacaagatc gccgaccagg tggttcgatt agcgcttttg
     2821 accaaagaag cgggaatgga tggagtagtt gcttctcccc atgaagtggc cgacattcgc
     2881 caggcctgcg gtcagcgctt cgtgatcgtc acacccggca tcagaccggc cgatagccaa
     2941 cgtaatgatc aacagcgcgt catgacgccc gcggatgcgg tgcgcgccgg cgtcgattat
     3001 attgtcgtgg gccgaccgat tctcgaagca caaaatcccg tcatggccgc gcgcggaatt
     3061 gtcgccgaaa tggaagaccg gctaaaatag ccgctcagag ttcaagctga cgcgacacgt
     3121 ttgtgcggcg ggccaccttg aactctattt ttttctgctc cagagccggt aaaaatggtg
     3181 taccgggcta tggcccgcgc caatcacgaa gccggcgcga atcgctcccg taatatattc
     3241 ttttgccagc gatactgctc tttcgagtgc ttcgccgcgg gcgaggtaag cggcgatcgc
     3301 agcagaaaac gtgcagccgg tgccatgggt atttctgctg tggatgcgcg ccgcccgcag
     3361 cacccggaaa cttttgccat caaagaagag atccagcgca gctcctttgc gatggccgcc
     3421 tttgatcacg accgtccttg ctcccatcgc gatgattcgg cgcgccgctt cttcgacatc
     3481 attaggcgtg cgcaggttca tcccggtgag ctgttccgct tccggcagat tgggcgtcag
     3541 gactgtcgcg agtggaacga gaagggaacg aagcgtggcg actgcatttt ttttgatcag
     3601 taagtctccg gaagtcgcca ccatcactgg atcgatgacg acgttcttaa agcggtgctg
     3661 ctggatcttt atcgccaccg cttcgataat tgacgcattg gcgagcatgc cggtcttgac
     3721 cgcatgcgcg ccgatatctt ccatcaccgc gtctatctgt cccgccacaa tctccggttt
     3781 cagcgctagg acttgggtga cgccgatcgt attctgcgcc gtactcgccg taatcgc
//
LOCUS       DASULZ010000714         2434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_589594, whole genome shotgun sequence.
ACCESSION   DASULZ010000714 DASULZ010000000
VERSION     DASULZ010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589594"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..247
                     /locus_tag="VFY96_14790"
                     /pseudo
     CDS             77..247
                     /locus_tag="VFY96_14790"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
     gene            272..601
                     /locus_tag="VFY96_14795"
     CDS             272..601
                     /locus_tag="VFY96_14795"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEX5607790.1"
                     /translation="MPADLLLRAKARIWIDYFSTRLHAAASDITHDRNPEKAQQRLRQ
                     YLTDLNREVADKTYLVGDQYSLADVSFIPFYTRRERYHVTIDDNYPHLKRWAENLIAR
                     PQVAATL"
     gene            621..1679
                     /locus_tag="VFY96_14800"
     CDS             621..1679
                     /locus_tag="VFY96_14800"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX5607791.1"
                     /translation="MNTLLVNPSEIRGIVTMAEAVEAVRLGFREWGENPQLNAPRRRI
                     HIPSGVRVSVHQGGVPAANATGLMTHCEWVKPMADEQIYPRLNHPVIVLYDSAEGELK
                     GIIIGEITCAELPNNIAVTGLRTAATSAVGTDLLARPDASRLGLFGAAGQAKNHLLAL
                     MQVRSLKDVRVYSRNPANRKKFADEMGPVTKLNITPVDSPEKAVRGVDIVLTATNASV
                     PVFDGNWLEPGQHVTTIVGSNVGLVKGGFAAAKRREIDDTTLARSHVHGIVSVQQAIQ
                     DEQADIYDPVNRGVIRWEQLIEIGEILAGNKEGRTKAEQITFYKNNAGQGVADVALGA
                     AVLEKIRVKHSGVQLQLE"
     gene            1872..>2434
                     /locus_tag="VFY96_14805"
     CDS             1872..>2434
                     /locus_tag="VFY96_14805"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX5607792.1"
                     /translation="MSKVAIAQVKSSIDKEENLRTAISMIEQAGRGGAELITFPEFLM
                     AFSPASQSGEELSKIAEAVEGRFISTLRETASKNRLAVLATLYETSTTPSRVFDTAVW
                     IDARGNIAMSYRKLHLYDAFGFRESDKFQPGSAVPPPVVSDECRFGVMICYDVRFPEM
                     ARMLALEGANILVAPAGWVQGEFKVDH"
BASE COUNT          584 a          695 c          664 g          491 t
ORIGIN      
        1 ggcagtgata attaagctca caacaggagg taaccagatg aaactttatt cctgccacac
       61 ctgaccctac gcacaccgga caaggattgt cctggcagag aaagaacttc cttacgagct
      121 gatcgaagtg gatctcaaaa ataagcccga cgatttgacc cgcaaaaatc cttacggcaa
      181 ggtgccggtc ttggtcgacg gcgacgggat catctatgaa tccgccatca tcgacgaata
      241 cctcgaagaa aaatttccca aggttcggct catgccggct gacttattgc tgcgcgcaaa
      301 agcgcgcatc tggatcgatt atttcagtac ccggcttcac gccgccgctt ccgacatcac
      361 tcatgaccgc aatcctgaga aagcgcagca acgactgcgg caatatttga ccgacttgaa
      421 tcgcgaggtg gctgataaaa cctatctcgt tggcgaccaa tattccttgg cggacgtcag
      481 ttttattccg ttctacactc gccgcgagcg ttaccacgtc accatcgacg acaactatcc
      541 ccatctgaaa cgttgggccg aaaacctgat cgcacgccca caggtcgccg ccacattatg
      601 aaaagttccc aagaagaacg atgaacaccc tgctggtaaa cccatcagag attcgcggca
      661 tcgttacgat ggcggaagcc gtcgaggccg tgcgcctggg atttcgcgaa tggggcgaaa
      721 acccgcagct gaacgcaccg cgccgacgaa tccacattcc gtccggcgtt cgggtgagtg
      781 tccatcaagg cggagtaccc gccgccaatg ctaccgggct gatgacgcat tgtgaatggg
      841 ttaagccgat ggccgacgag caaatctatc cacgcctcaa tcatcctgtg atcgtgcttt
      901 acgattccgc cgagggcgag ctcaagggca tcatcattgg agagatcacc tgcgccgagc
      961 tgccgaacaa tatcgcagtt accggcttgc gtaccgcagc cacgagcgcg gtgggcaccg
     1021 acttgttggc gcgtcctgac gcgtcgcgcc tcggcctttt cggtgccgcc ggtcaggcca
     1081 aaaatcatct gctggcgctg atgcaagtac gaagtctcaa agacgtcaga gtgtacagtc
     1141 gaaatccggc caaccgaaaa aaattcgcgg acgaaatggg acccgttaca aagcttaaca
     1201 tcactccggt cgattcgcct gaaaaggctg tgcgcggcgt cgatattgtc ttgaccgcca
     1261 ccaatgccag cgtgccggtc ttcgatggca attggctcga acccggccag catgtaacca
     1321 cgattgtcgg cagcaatgtc ggtctcgtga aaggcggctt cgcggcggca aaacggcggg
     1381 aaatcgacga tacgactttg gcgcgcagcc acgtccatgg catcgtctct gtccaacaag
     1441 cgattcaaga cgagcaagct gacatttacg atccggtcaa tcgcggcgtg atccgctggg
     1501 agcagttgat cgaaatcggt gagattcttg ccggcaacaa ggaaggccgc accaaagctg
     1561 agcaaatcac tttctacaag aataatgccg gccaaggcgt cgcggacgtt gcgctcggcg
     1621 ccgcagtgtt ggaaaaaatc agagtcaagc actccggagt gcaactgcag ttggagtgat
     1681 ggagtggtga agtaatgggc gggagtccag cactccaatt actccactct ccactccggc
     1741 attcttctcc gcgcgtcctc ggcccgcatg accgaagtcg caatcgcggt caacgtcatt
     1801 gccgcaagca ccagccatgg gctcttggat agcgtgaact aattactgat tccacagaca
     1861 gtcggaaatt tgtgagcaaa gtcgccatcg cccaagtcaa atcctcgatc gacaaagagg
     1921 aaaatctccg caccgcgatt tcgatgattg aacaggcagg ccgcggcggc gcagagctga
     1981 ttacttttcc agagttcctg atggcgttct ctcccgcgag tcaaagcggg gaagaactgt
     2041 cgaagatcgc cgaagccgtc gagggacgct ttatctccac gctgcgcgaa accgccagta
     2101 aaaaccggct cgccgtgttg gcgacacttt acgaaacctc gacgacccca agccgggtgt
     2161 ttgacaccgc agtgtggatc gatgcgcgcg gcaatatcgc catgagttac cggaaactgc
     2221 atctctacga cgccttcggg tttagggaat cggataagtt tcagcctggc agcgccgttc
     2281 cgccaccggt ggtctctgac gaatgccgct tcggcgtgat gatttgttac gacgttcgtt
     2341 ttccggaaat ggcgcgcatg ctcgcccttg aaggagcaaa tattctggtt gcgccggcag
     2401 gctgggtgca aggcgagttt aaagtggacc attg
//
LOCUS       DASULZ010000715         2906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_690775, whole genome shotgun sequence.
ACCESSION   DASULZ010000715 DASULZ010000000
VERSION     DASULZ010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690775"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1453)
                     /locus_tag="VFY96_14810"
     CDS             complement(179..1453)
                     /locus_tag="VFY96_14810"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5607793.1"
                     /translation="MISKEENELLTKTGRGTPAGELMRRYWQPVALAEELPRGGAPLA
                     VRICAEDLVLFRDDQERIGLLGIHCAHRGTDLSYGRIEDGGLRCLYHGWLYDIHGRCL
                     EQPGEPGGGEHRDQIRQRAYRCIEKGGAIFTYMGPGEPPLLPNYDFLNASPDHLYTSK
                     IYHDCNYQQANEGNIDPVHLSYLHRFLENRNEPYRGVRGVAESHYNLVARNSAPTIDV
                     ELTDFGVRIYTVRKLEADKCYLRVSYFILPNLSAFPGQTGGDGYSVNWHVPIDDTHHW
                     KYTFVYSAGAPLKKDVVSRERSELDANFRLARNPSNRFLQNREAMKTKTYAGMGSGFQ
                     AHDAFATASQGAVQDRSDEHLVSSDKAIVAARKLLEKAIHDVRDGREPPHVVRSAAQN
                     RFSHLLVLSDMIPTGDWKEYTKYLEAEARARI"
     gene            complement(1561..>2906)
                     /locus_tag="VFY96_14815"
     CDS             complement(1561..>2906)
                     /locus_tag="VFY96_14815"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX5607794.1"
                     /translation="ESTAELVPQPPMLIFDKVKGYPAGYRVCSLPYAAYRRVALALGL
                     PTDKSKLEILRMAARKVKNAEPIAPKEVSTAPLFENKLKGSDVNLLKFPALRFHENDG
                     GRYFGPGGGLTNRDPDSGYINSGTYRMQLHDQNTLGLWMSPGQNGRQICAKYWQQGKS
                     CPVVASYGQHPLVFMPSYTKFPYGRSEMEISGGLIGRPLEVLRGPITGLPIPASSEIA
                     IEGEIPPPSEEARPEGPFGEWPGYYSGGTSGTGENQPIIRVKAIYHRNEPILEDEAPL
                     WPGAVKMDLHISAGVLWDQLESAGVQDIAGVYNHTSYLTVISIKQKYAGHAKQAGTAA
                     LSCAAANRNGRYVVVVDEDIDPTNIKEVLWAMMTRVDPPTNIDIIDGCWSTPLDPRMP
                     PEKRDNKDHTNGRAIFYAVRPFAWKEKFPKVSRSSRELRERTIAKFKNVIPFPGV"
BASE COUNT          597 a          868 c          793 g          648 t
ORIGIN      
        1 cacatcgctt gaacgctcgt gtaggtttcg cccatgtaaa ctgcacggaa tttttccagg
       61 gcgtcaggac gcgtatccgc cgccgcggtg actttgatga acgacaacgc cgagaccggt
      121 ggcagtatct gcgtgctggc gacgccgagc cccgcgatgc cgagacgaag aggttcgctc
      181 agattctcgc cctcgcttcc gcttcgagat attttgtgta ttccttccaa tcgcctgtcg
      241 ggatcatgtc agaaagcacc agcagatggg aaaatcggtt ttgcgccgcg gaccgcacga
      301 cgtgcggcgg ctcgcggccg tcacggacat cgtgaatggc cttttccagc agcttgcgag
      361 cggcgacaat ggctttatcg gaggaaacca ggtgttcgtc gctacgatcc tggactgcgc
      421 cctggctggc ggtcgcgaaa gcatcatgag cttgaaaacc ggaacccatg cccgcatagg
      481 tcttggtttt catcgcttca cgattctgta gaaaacgatt cgacggattg cgcgccagtc
      541 gaaaattggc atccaactcg gagcgctcac gactgaccac gtcttttttc agtggcgcgc
      601 cggcgctgta aacgaacgtg tacttccagt gatgcgtatc gtcgatgggt acgtgccaat
      661 tgaccgagta accatcgccg ccggtttgtc ccggaaaagc gctcaggttg ggcagaatga
      721 aataggaaac acgcagataa cacttatccg cttcgagctt tcgcaccgta tagatgcgca
      781 cgccaaaatc cgtcagctcg acgtcgatcg taggcgccga gtttcgcgcc acaagattgt
      841 agtgcgactc cgccacgccg cgcacgcctc tgtaaggctc gttgcgattt tccaggaagc
      901 ggtgcagata cgataaatgc accggatcga tgttgccttc attagcctgc tgataattgc
      961 agtcgtgata aatcttcgag gtgtaaagat gatcgggaga ggcattgaga aaatcgtaat
     1021 tcggcaataa cggcggctct cccggtccca tgtacgtgaa gatcgcgccg cctttctcga
     1081 tacagcgata ggcgcgctgg cgaatctgat cgcgatgctc cccaccgccc ggctcgcccg
     1141 gttgctcgag gcatctccca tggatgtcgt aaagccagcc gtgataaaga cagcgcaatc
     1201 cgccatcctc gatgcgccca taactcagat cggtgccgcg atgcgcgcaa tgaatcccca
     1261 acaacccaat tcgctcctga tcgtcgcgga acaaaaccag atcctcggcg caaattcgga
     1321 cggccaaagg cgcgcctccc cgaggcagtt cctcggcgag cgctaccggc tgccagtacc
     1381 ggcgcatcag ctcacctgcc ggtgtgccgc gcccggtttt ggtcagaagc tcgttttctt
     1441 ctttactgat catgagagcc ttcctcgcac acccggccct agttcagctc tgtgacgata
     1501 gcaaatttga caatagatcc ttcgctatgc gcagcgaaaa atctcgcttg cgcttttcgc
     1561 tcacacaccc gggaacggga ttacattctt gaacttcgca atggtccttt ctctcagctc
     1621 acggctcgac cgactcactt ttggaaactt ttctttccag gcgaacggtc gcaccgcata
     1681 aaaaatcgcg cgcccattgg tatggtcttt gttgtcgcgc ttctcaggcg gcatgcgcgg
     1741 atcgagcggc gtggaccaac aaccgtcgat aatatcgatg ttcgtcggcg gatcaacgcg
     1801 ggtcatcatc gcccagagca cctccttgat attggtcgga tcgatgtctt catcgacgac
     1861 aacaacgtaa cgtccgttgc gattggccgc cgcgcaactg agcgccgccg tcccagcctg
     1921 tttggcgtgg ccggcatatt tttgcttgat cgagataact gtaagatacg aagtgtgatt
     1981 gtaaacaccg gcgatgtcct gaaccccggc gctctccaac tggtcccaga gcacgccggc
     2041 tgagatatgc aaatccattt tgactgcgcc tggccaaagc ggcgcttcgt cttccaggat
     2101 cggttcgttt cggtgataaa tcgccttgac ccggatgatt ggctgattct ctcctgtgcc
     2161 gctggtgccg ccggaatagt atcccggcca ttcgccgaat ggaccctcag gccgtgcttc
     2221 ctcggagggc ggcgggattt caccctcgat tgcgatttcg gatgaggctg gaatcggcag
     2281 gccggtaatg ggcccgcgta agacttccag cggtctacca atcaatccac cggagatttc
     2341 catctcggac cggccataag gaaatttcgt gtacgacggc atgaacacca gtggatgctg
     2401 gccgtagctc gccaccaccg gacagctctt tccctgttgc caatacttgg cgcaaatctg
     2461 acggccgttt tgcccggggc tcatccacaa gccgagggtg ttctggtcgt gcaactgcat
     2521 ccggtacgtg ccgctgttga tgtacccgga atccgggtcg cggttcgtca gcccgccgcc
     2581 tggaccaaaa tagcgtccgc cgtcgttctc gtgaaaccgc aaagcgggaa atttgagcag
     2641 attaacatcg ctgcccttga gcttattttc gaacagcggc gctgtgctca cttcctttgg
     2701 cgcgatcggc tcggcattct tcaccttgcg ggccgccatg cgcagtatct cgagcttcga
     2761 cttatccgtt ggcaatccca acgccaaagc cacacggcga tacgctgcgt agggcaaact
     2821 gcaaacccgg taaccggcgg gatagccctt cactttgtcg aagatcagca tcggtggctg
     2881 cggcaccaac tcggcggtcg attcga
//
LOCUS       DASULZ010000716         2093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_791481, whole genome shotgun sequence.
ACCESSION   DASULZ010000716 DASULZ010000000
VERSION     DASULZ010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791481"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..1204)
                     /locus_tag="VFY96_14820"
     CDS             complement(230..1204)
                     /locus_tag="VFY96_14820"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607795.1"
                     /translation="MMVRKILSILVLLISLLAGWVLPVAAQIDFPIGYVSRGGTYAFI
                     TLIEQHKLLEQEGIKPTFVYIGGPQVSQALISGDIRMAIVAAASPIRAAAHGAELRFV
                     AGVTDKEVASFVTAANIKTPADLKGTRLAIDRLGDYSDFRARKVLEIFGLQAQKDVTL
                     LQIGAQTARFAALRSGQVQSTFVVPPLTLVARKAGLRELADLGELGFPSSSGAFVVMQ
                     STIERQAREIYGVLRAVAKAIRIYKTDKEQGIGAIAQFMRLNDREALEETWQMNAKVL
                     KDNPSPPMAGIRVVRDFLAQTDPEVKKMNLESFITTQFTDRLQREGVK"
     gene            complement(1411..1809)
                     /locus_tag="VFY96_14825"
     CDS             complement(1411..1809)
                     /locus_tag="VFY96_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX5607796.1"
                     /translation="MTRLEVATKRKRQVIDLTDLCQEKLSELKKSSGMCHLFVLHTTA
                     ALTIADLDPGTDLDLLDALDALIPKLKYRHPHDPSHVSDHILSSLIGTSLNVPVDDGE
                     LLLGTWQRIILVEFDGPRKRQIALSFVSEI"
BASE COUNT          490 a          535 c          594 g          474 t
ORIGIN      
        1 gtattgcgcc gtgagtcctg ccttttcgaa gaatttcttg tcaatggcca ccagatacgg
       61 aacgctggtc aaagtcatcg atgatgtagc gatgacgatg tctttggcgt gaagcggaaa
      121 acaggacaac agccaaagag caagacaaag acaaaaagga aaatggaaag aaatcatcgc
      181 gcgctcaaaa attcgtctcg tcatcgagaa tatatcgagc cggccaattt tacttcacgc
      241 cttctctttg cagacgatcg gtgaattgag tggtgatgaa ggattcgagg ttcatctttt
      301 tcacctcggg atcggtttgg gccaaaaaat ccctgacgac gcggataccg gccattggag
      361 gagacgggtt atccttcaac actttcgcgt tcatctgcca ggtctcttcc aacgcttccc
      421 gatcattgag cctcatgaat tgcgcgatgg cgccgatgcc ttgttctttg tcggtcttat
      481 agatgcggat cgctttcgcg acggcgcgga gcacgccata gatttctctg gcttgacgct
      541 cgatggtcga ttgcatgacg acgaaggcgc ccgaggagga gggaaaaccg agctcgccaa
      601 gatcggcgag ttcgcgcaag ccggcctttc tcgccaccag ggtcaaaggc ggcactacga
      661 aagtcgactg cacttgcccg cttctgagcg cggcgaagcg agcggtttgc gcgccgatct
      721 gtagcaaggt cacgtctttc tgcgcctgga gcccaaatat ctccagcact ttacgggcgc
      781 gaaaatcgct gtaatcgccg agccggtcga tggccaaccg cgtgcctttc aagtcggcgg
      841 gagttttgat attcgccgcc gtgacgaagg acgcaacttc tttatcggtc acgcccgcaa
      901 cgaagcgcag ctcggcgcca tgggccgcgg cacggatcgg actcgccgcc gcaacgattg
      961 ccatgcgaat atcgccggag atcagcgcct gcgaaacctg cggtccgccg atataaacga
     1021 acgtcggttt gatgccttcc tgttccaaaa gcttgtgctg ctcgatcaag gtaataaacg
     1081 cataggtacc gccgcgcgag acatagccga tgggaaagtc gatttgagcg gcgacgggca
     1141 aaacccagcc tgcgagaagt gagatcaata agaccaggat cgataagatt ttccgaacca
     1201 tcattgcgcc agttcacctc aagttgcgat gctatacagt tccgcgtagc aactgagttt
     1261 ttcagcgcga acccgctttt atcaatggcc aaggttcagg tcaacgaacc ccatgctcga
     1321 acattcggca ggtgtttcgt tgggtcgagc ggaaagctcg ccaataagga caaattaggc
     1381 tggtgcgtga cggctgatga tccgcaatca tcagatctcg gatacaaacg acagcgcaat
     1441 ctgccgttta cgcggaccgt cgaattcgac gaggataatc ctttgccatg ttccgaggag
     1501 gagctcgccg tcgtcaaccg gcacattgag ggaggttccg atgagcgagg aaaggatgtg
     1561 atcggagacg tggctcggat cgtgcggatg gcgatatttg agtttcggga tcaaggcatc
     1621 gagcgcgtcg agcaaatcca gatctgttcc gggatccaag tctgcaatgg tcagggcagc
     1681 ggttgtgtga agaacgaaca ggtgacacat gccgctgctt tttttcagct cggacagttt
     1741 ctcttgacac aaatcggtga gatcaatcac ttgtctcttg cgctttgtgg cgacttccaa
     1801 tcttgtcatg ggttcagctt accgtttggg atgattcatt caatgatcga gttaactggc
     1861 cgccgcccct catccttgcc gtctttcatc agaatgggcc agggaggttt cgatcgaacg
     1921 aggggaatct acatgtgctg atgaatcgga tgccgaagga cggacacgac gtcagtcaat
     1981 ttgttgaaaa tattagagac ttgcgttagt ttgacagagt cgtggggtgg gaggcttgat
     2041 cgctgggcga agttctactc agcacatcaa cgcacggcgt gtcgcagcgc cga
//
LOCUS       DASULZ010000717         4145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_589664, whole genome shotgun sequence.
ACCESSION   DASULZ010000717 DASULZ010000000
VERSION     DASULZ010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589664"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="VFY96_14830"
     CDS             <1..1167
                     /locus_tag="VFY96_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HEX5607797.1"
                     /translation="PPSLFSQDAEQPVVFKQEELEQLVAPIALHPDSLIAQILMASTY
                     PLEVALADRWTKQNTTLKGDTLTKALEGQDWDPSVKSLVNFPQVLTMMSEKLDWTQKL
                     GDAFLADQKKVLDTIQSLRAKAQAAGNLQTNQEQKVIVEEKIIKIEPANPQVVYVPTY
                     NPTVVYGSWPYPAYPPYYYYPPGYVATSMMAFGAGVAMGAAWGYAWGNTNWNGGDVDI
                     DVDRNTNINQNINRDQAKQKMQERGQGNQRGQGKWQHSPEHRKGVSYRDQGTAAKFNR
                     VSTNDSIKSREQFRGRADQGRQDLSRGNVGDRGSVGDRGGAGGLADRGGGQLGAGNRA
                     GTTDRGGAFSGVSGGGSSARAASSRGSASRGSFSGGGARGGGGGRGGGGGGRRR"
     gene            1220..2173
                     /locus_tag="VFY96_14835"
     CDS             1220..2173
                     /locus_tag="VFY96_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2950 domain-containing protein"
                     /protein_id="HEX5607798.1"
                     /translation="MILAGNKRQKRSSRLRNYLAVIATFAVLVIGAVPAFAQKAFSSP
                     EEAVKSALAAARSNNDNELLAIFGPGAKEVLFSGDPVADKQRRAEFIAAYDQVNRLST
                     EGEDRILIVGKQDWPFPIPLVKKGQSWVFDTEKGKQEVLNRRIGENELFTIQTMLAIV
                     DAEREYAMKDRNRDSLLEYAQKFVSAPGKKDGLYWSAKDGEPQSPLGPIMTQAREQGY
                     RAAPPSASPYPYHGYHYRILTAQGKDAPGGAYSYLVKGKMIGGFAAVAYPAEYGNSGI
                     MTFIVNHDGKVFQKNLGPNTASVAKGMKEYNPDKTWTEVKQ"
     gene            2283..2792
                     /locus_tag="VFY96_14840"
     CDS             2283..2792
                     /locus_tag="VFY96_14840"
                     /inference="COORDINATES: protein motif:HMM:NF028053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PliI family lysozyme inhibitor of I-type
                     lysozyme"
                     /protein_id="HEX5607799.1"
                     /translation="MTVARIVAPLRQRTEVALTVFLLVVSATVLSAAEADHRFVQLFR
                     FPDSRETVVIVEGDLEPRSIGSYTMRIYKGRSAKFPTDEFITGIVRPRIGTIDAVHFA
                     DVDGDNRADLIVMMRSAGSGGYLTAEAFRYHDSSLELIGSVAGLYKTADVIQVLREKL
                     KESMGVEGH"
     gene            2945..3682
                     /locus_tag="VFY96_14845"
     CDS             2945..3682
                     /locus_tag="VFY96_14845"
                     /inference="COORDINATES: protein motif:HMM:NF025724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4410 domain-containing protein"
                     /protein_id="HEX5607800.1"
                     /translation="MLNPVRALSGGIVVCFAVACGQSGIRNTTLRTSANLPRPERILV
                     YDFAVSEHDVKEYQGIMRQQPTIKDAAERERLLAQEVKDALAVELIDGLKSLGFVAER
                     VQRGTVATHNEMLIDGQFLTVDEGNPLHSLVIGFGSGASSVQTQVQVYQAPNARKIME
                     FTTQSDSSKMPGAATTMAAGAAVAGGVTAGMVAANAAASGVKTYKSDVARMAADTGDQ
                     VARYLSEAFAKQGWIRPDQVRKARIVY"
     gene            3718..4095
                     /locus_tag="VFY96_14850"
     CDS             3718..4095
                     /locus_tag="VFY96_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607801.1"
                     /translation="MKSYDAYMMKSQKFIRTTIGFSLLIVAALAGCTTSEPVPVPAPS
                     TFDRSWSAAVSAAQDEGVRITSEDRGSGVIRGVRTDQEVTINLRSQADGSVRIEIGSR
                     GPKGSDPGLANRISRAYDRRMGR"
BASE COUNT          998 a         1049 c         1272 g          826 t
ORIGIN      
        1 ccaccgagtt tgttttcgca agacgccgag caaccggtgg tattcaaaca ggaagaactg
       61 gagcagctcg tagcgcccat cgcgctccat ccggattcac tcattgcaca aatcttgatg
      121 gcgtcgactt atcctttgga agtcgcgctc gccgatcgct ggacaaaaca aaataccact
      181 ctgaagggtg acacgctgac caaggcgctt gaaggccagg attgggatcc gagcgtcaaa
      241 tcgctggtca attttcctca ggtgctcacc atgatgagcg aaaagctcga ctggacgcag
      301 aaactcggcg atgcgttttt ggcggaccag aaaaaagttc tcgataccat tcagagcctc
      361 cgggcgaagg cacaggctgc gggtaatctc caaacgaacc aagagcagaa agtaatcgtc
      421 gaggaaaaga tcatcaagat cgagcccgcc aatccacaag tcgtttatgt gccgacttat
      481 aatcccacgg tggtgtatgg ttcgtggccg tatccggctt acccgcccta ctactattat
      541 ccgccagggt atgtggctac atcgatgatg gcgttcggcg cgggtgtagc catgggagcg
      601 gcgtggggtt atgcatgggg taacaccaac tggaacggtg gagatgtcga catcgacgta
      661 gaccgaaaca ccaacatcaa tcaaaatatc aaccgagacc aggccaaaca gaaaatgcag
      721 gagcgaggac aaggcaatca gaggggtcag ggaaagtggc agcatagccc ggagcaccgc
      781 aaaggtgttt cttatcgaga tcaggggacg gccgcgaagt tcaaccgggt gagcactaac
      841 gattcgatca aatcgcgtga acagtttcgc ggccgagccg accaaggaag gcaggatctt
      901 tcgcgtggta acgtgggcga ccggggcagt gtcggtgatc gcggtggcgc aggcggtctc
      961 gctgatcgtg gcggtggtca acttggcgcc ggaaatcgtg ccggaacaac ggaccgcggc
     1021 ggcgcctttt caggtgttag cggtggcggt agctcggcaa gagcggccag ttctcgcggc
     1081 agtgcgagcc gcggtagttt tagtggaggt ggtgcaagag gcggcggcgg cgggcgcgga
     1141 ggaggaggcg gtgggcggcg ccgataagga gctgtggacg atgggacgat tccggttcgg
     1201 agtgaacatg gaggatgcaa tgattctggc aggtaacaaa agacaaaagc gctcgagtcg
     1261 gttgcggaac taccttgccg tgatcgccac attcgccgtg cttgttatcg gtgccgtgcc
     1321 cgctttcgca caaaaagcgt tttcctcgcc ggaggaggcg gtcaaatccg ctctcgctgc
     1381 ggcacggagc aacaacgaca acgagctgct tgcgatcttt ggtccgggag cgaaggaagt
     1441 tttgttttcc ggagatccgg tggcggacaa gcagagaaga gccgaattta tcgcagccta
     1501 tgaccaggtg aaccgtctta gcacggaagg agaagatagg attctgatcg tcgggaagca
     1561 ggattggccg tttcccattc cgcttgtgaa aaaagggcag tcttgggtct tcgatactga
     1621 aaagggcaag caggaagtcc tgaaccgcag aatcggcgag aatgagttgt tcacgatcca
     1681 aacgatgctt gcgatcgtcg atgccgaacg tgagtatgcg atgaaggatc gcaatcgtga
     1741 cagccttctc gaatatgcgc agaagttcgt cagcgcgcca ggaaaaaaag acgggttgta
     1801 ctggtccgca aaggacggcg aaccgcaaag tcctctcggg ccgatcatga cccaagcgag
     1861 agagcaaggt tatcgagcag caccgccctc cgcttcaccc tatccttatc atggctatca
     1921 ctatcgaatc ctaacggcgc agggtaagga cgcccccggg ggcgcgtaca gctatcttgt
     1981 gaaaggcaaa atgatcggtg gcttcgccgc tgtggcctat ccggcggagt acggcaattc
     2041 cggcatcatg acttttatcg tcaatcacga cggtaaagtc tttcagaaaa atctcggacc
     2101 gaataccgcg tccgtggcga aaggcatgaa ggagtacaat ccggataaaa cctggaccga
     2161 ggtgaagcag tgaggaagcg gccttaaagc ggagggtaac gtcaaacggc gaagccgccc
     2221 gctctcggat cttggcgtcg tgcctaagct gtatcgtcgc agtagtttgg attccagaga
     2281 acttgaccgt agcccgaatt gtcgcgccgt tacgccaaag gacagaggtt gcgctcacgg
     2341 tttttcttct tgtggtcagc gcaaccgtcc tgtctgcggc agaagcagat catcgctttg
     2401 tccaactctt tcgctttccc gacagcagag agacggtggt gattgtcgag ggtgatttag
     2461 aaccgcgcag catcggcagc tatacgatgc gcatttacaa aggacgctcg gccaaatttc
     2521 ctacggatga attcatcacc ggaattgtcc ggccgagaat cgggaccatc gacgctgtac
     2581 atttcgctga tgtggacggc gacaatcgag cagatcttat cgtgatgatg cgctcggcgg
     2641 gttctggcgg atatctgaca gccgaggcat ttcgatatca cgacagctcg cttgaactga
     2701 tcggctcggt cgcggggctg tacaaaacag cggatgtgat tcaggtactg cgcgaaaagc
     2761 taaaagagtc catgggagtg gaaggccatt agatcctcgg cgagattccg tcctgtcagc
     2821 ttttttcaag cttcagttta tcattgatca acggtcgcag ttaaattcct gccaagtttt
     2881 aggaatcgaa ggcgcgacga ggattccgga cggaagataa caattttaaa ggagggataa
     2941 aaggatgttg aaccctgtgc gagctttgag cggcggaata gtggtttgtt tcgcagtcgc
     3001 atgcggacaa tcgggaattc gcaataccac gctccgaacg tcagcgaatc tgccccggcc
     3061 cgaacggatt ctcgtctatg acttcgcagt gagcgaacac gatgtcaaag agtaccaagg
     3121 catcatgcgg cagcaaccga caatcaaaga tgcagccgag cgggaacggc tgctcgcgca
     3181 ggaagtgaaa gatgcgctag cggtggaatt gatcgatggt ttgaaatcgc tgggctttgt
     3241 cgccgagcgt gtccagcgcg gcaccgttgc cacccataac gagatgctga tcgatggcca
     3301 attcttaacg gtcgacgagg gcaatccgct acacagtctg gtgatcggct ttgggagcgg
     3361 cgcctcctcc gtgcagaccc aggtccaagt ctaccaggcg ccgaatgcgc gaaaaattat
     3421 ggaattcacc acacaatcgg atagcagcaa aatgcctggc gcggcaacca caatggcagc
     3481 aggtgcggcg gtggctggcg gagtgacggc cggaatggtt gccgccaacg cggctgcgtc
     3541 aggggtgaag acttataaat cggatgttgc ccgaatggcg gccgacactg gggatcaagt
     3601 ggcgcgctat ctgtccgagg cttttgccaa acagggttgg attagacccg accaggtgcg
     3661 caaggcgaga atcgtctact gaattccgtg cacgcggagc cgggaaggtg gtgatgtatg
     3721 aaatcttatg acgcttacat gatgaagtca caaaaattca tccgcacgac gatcgggttc
     3781 tcccttttga ttgtcgcagc acttgcgggt tgcacaacgt ccgaacccgt tccggtaccc
     3841 gcgccgagca ccttcgatcg ttcctggagc gcagcggtgt ccgcggcgca agacgagggt
     3901 gtgcggatca cctccgaaga ccgcggcagc ggagtgattc ggggcgtcag aaccgaccag
     3961 gaagtcacga tcaatcttcg cagccaagcg gacggcagcg tgcgtatcga gatcgggtcg
     4021 cgcggtccca aaggatcaga tcctggattg gccaacagga tatcgcgcgc ctacgaccgg
     4081 cgcatgggcc gatagtgtat tggaccaaag tccaatgtac gcgcggctgg tgatttatta
     4141 agaac
//
LOCUS       DASULZ010000718         2039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_488152, whole genome shotgun sequence.
ACCESSION   DASULZ010000718 DASULZ010000000
VERSION     DASULZ010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488152"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..1233
                     /locus_tag="VFY96_14855"
     CDS             214..1233
                     /locus_tag="VFY96_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007229116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HEX5607802.1"
                     /translation="MRNGKIRIGIMGLGQIGRHLYHLAREIDDIEIVAVADIGKPEII
                     QYLLNSDGVDEAPCELQDNYLINEKFRTRMLQLARPDEVPWDVLNVHCVVDATGRYRS
                     RRDMEAHLRAGAPRVILASLPAESIDRIVVPGINEGSAGAADKMISAGSATTNAFALL
                     LQSLDQALGVDCASMTTVHAYSSDQSLQNYAHKDFRRSRSAAENIIPNTNESARWVEL
                     VLPKFEGKLSGYALNVPVQRGSLLDVNCVMQAGTVTAEQVNGAMRAAAADHPTLIGVT
                     DDPIVSSDVIGCRQSLLFDSLATIKAGKRIIKVLAWYESLGHACRVLEVVRHYGRLDA
                     IGGGR"
     gene            1230..>2039
                     /locus_tag="VFY96_14860"
     CDS             1230..>2039
                     /locus_tag="VFY96_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009022881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HEX5607803.1"
                     /translation="MRVAINGFGRIGRSVFRILDKRADAELVAINDLFDHDALRYLLN
                     YDTVMGRFEGALRLEGNELVTSHGRTRMLREKEPLKLPWRELDIDVVIESTGIFTTRE
                     QLAKHLDAGAKRVVLTVPAKDEIDYTVVIGVNDDGLKPEHRMISNASCTTNCLAPLAK
                     VLHDSFGIVEGIINTVHAYTNDQRLADVPHSDWRRSRAAAENIIPTSTGAAKAVGEVL
                     PELKGRLDGIAARVPVPDGSVVDLFVELQKSVSAEDVHGAIKSAAESKRMKG"
BASE COUNT          452 a          550 c          605 g          432 t
ORIGIN      
        1 gccgaatcac tttcactttc tgattgaagc ttcgcatcac cagtctcagc gaaatgagga
       61 agtagataaa aaagtagcct tgtccccttt acacctccag tttgacgatt tcctcagaaa
      121 agcattttct tttggttgat cggttgttag agttctcggc tatggataac gccaacttcg
      181 agcgcttaat tttgcggaaa cggaggcggc caaatgcgta atggtaaaat ccgaatcggc
      241 attatgggat tgggccaaat tgggcggcac ctgtatcacc ttgcgcggga aatagatgat
      301 atcgaaatcg tcgcggtggc tgatatcggc aaaccggaaa tcattcagta cctgctcaat
      361 agcgacggcg tggacgaggc gccctgcgag ctccaggaca attacctgat caacgagaag
      421 ttccgcacaa gaatgctcca gcttgcccgg ccggacgagg ttccatggga tgttctgaac
      481 gtccactgtg tcgttgatgc cacgggcaga tatcggagtc gtcgggacat ggaggcccat
      541 ctgcgcgccg gtgccccgcg ggtcattctg gcgtcgctgc cggcggaatc aatcgaccgt
      601 atcgtcgtac cgggaatcaa tgaagggagc gccggcgccg cggacaaaat gatttcggcc
      661 ggttccgcga cgaccaatgc atttgcgctt ttattgcaat ctctggatca ggccttagga
      721 gtcgactgcg catcgatgac gacggtccac gcctatagta gcgatcagtc gctgcagaat
      781 tatgcacaca aagatttccg gcgcagccgg tccgcggcgg agaatatcat ccccaacacc
      841 aatgagtccg cgcgatgggt ggagcttgtt ctgcccaagt ttgagggaaa gttgagcggt
      901 tacgccctca acgtgccggt ccaacgcgga tcattgctcg atgttaactg cgtcatgcag
      961 gctggcaccg tcacggccga gcaagtgaac ggagctatgc gcgccgccgc tgccgatcat
     1021 ccgacgttga tcggcgtgac cgatgatccg attgtttctt cggatgtcat cggttgccgg
     1081 caatccttgc tcttcgattc gctcgccacc attaaagcag gaaaaagaat catcaaagtg
     1141 ctggcctggt atgagagcct ggggcatgcg tgccgcgtgc ttgaagtggt ccggcactat
     1201 ggcaggctcg atgccatcgg aggtgggcga tgagagttgc cattaatgga ttcggtcgaa
     1261 tcggccgttc tgtgttccgt attctcgata agcgggcgga cgccgaactg gttgcgatca
     1321 acgacctttt cgaccacgac gcgctgcgat acttgctcaa ctatgacacc gtgatgggtc
     1381 gcttcgaggg cgccctgcgg ctcgaaggca atgaactggt gacatcgcac ggtcggacgc
     1441 gcatgcttcg tgaaaaggag ccgctcaaac tgccctggcg cgagctcgac atcgacgtcg
     1501 tcatcgaatc gaccggaata ttcactacca gggaacaact tgccaagcat ctggatgcgg
     1561 gagccaagcg agtggtgctg actgttccgg cgaaggatga gatcgattat accgtcgtaa
     1621 tcggcgtcaa cgatgacggg ctcaagcccg agcacaggat gatttctaac gcgagctgca
     1681 ccaccaattg tcttgcgccg ctggccaagg tgctccacga ttctttcggt atcgtggaag
     1741 gaatcatcaa taccgtgcac gcctacacca acgatcagcg cctcgccgac gtgccgcact
     1801 cggattggcg gcggagccgc gccgcggcgg aaaatattat accgacatcc accggagcgg
     1861 ccaaggcggt gggcgaggtc ttgcctgaat tgaaaggacg gctggacgga atcgccgcgc
     1921 gagtcccggt gcccgatggc tccgtggtcg acttgttcgt cgagctccag aaaagcgtta
     1981 gtgccgagga cgttcatggc gcgataaaat ccgcggcgga gtcgaaacgg atgaaaggc
//
LOCUS       DASULZ010000719         2275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1502887, whole genome shotgun sequence.
ACCESSION   DASULZ010000719 DASULZ010000000
VERSION     DASULZ010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502887"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..1108
                     /locus_tag="VFY96_14865"
     CDS             260..1108
                     /locus_tag="VFY96_14865"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5607804.1"
                     /translation="MESELKQLSGSVFALIGPQGATNFTIIKNRAGHAVLIDADIRRI
                     DEIEEALQLAGCSRVEYLLDTHEHFDHTSANFYFARRGIPIVASSRCAGAMREQGGPD
                     FERMMKPVPELYERFPGLCLTLPDVQFTDRTQVTLPGVTLHLKYRAENGHSHSKGDTT
                     IYFEEEEVFIAGDLLYTEVHPVTFFGNIPNWLSSLKPLFDSHYQQLVPGHGPAVDGEQ
                     TGRAYFKQMYDYLEDFYGHLQELEAGRESSDHVAQHMLSGADAKLGKTRMVERNINQF
                     LTGKWF"
     gene            1328..>2275
                     /locus_tag="VFY96_14870"
     CDS             1328..>2275
                     /locus_tag="VFY96_14870"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607805.1"
                     /translation="MIVIIACVFWLSTHGHAADKLERLRAAYPAVAPGSTPSWITAEK
                     KIWSKYGYDVETILVSGGARAVPALVSGSVQFLFGSDTGITTAQLQGIPVVRLGVTMN
                     TLGSSLITQPGIQSVQELKGKVLGISRGRDASYVRLAKLLRDNGLNPNDDVKFLSIGG
                     GEAGRLSAVKAGVIHGTMLFPPLDLIARNDGLKVLAKFDVPTPGGGINTTATLLKQNR
                     SMVVNFLKGYIEGIHYMKTHKAESLHVLQKYFQNSDMTAMAYLYDETTGRLEKDLRAS
                     PESIRFHLEMAGLDDPRAAKLSEKEFWDPSVVEEIRRSGF"
BASE COUNT          505 a          662 c          600 g          508 t
ORIGIN      
        1 catcattgcg cagctcttcg aaaaccgtga gcagctcgcg tatgacatcc cggctcagag
       61 cattccgctt ctccgcccga ttgatcgtaa tcttcgccac tgtgtctcgc gtttcgagaa
      121 ttgtaaattt gccttccctg atcatgaatt ctacctctca taactttcca aagtttatag
      181 gcgaataaca tcgcggttac ctgtcgtcaa ccagtgatcg gcttgcaacc tgagatcgag
      241 tcgggtaagg cgatctctta tggaatccga gctgaagcaa ctcagtggat ccgtttttgc
      301 gctgatcggg cctcaaggtg cgaccaactt tacgatcatc aagaatcgcg cgggccacgc
      361 tgtcctgatt gacgccgata tccggcgcat cgacgagatc gaagaggctt tgcaactcgc
      421 aggctgttcc agagttgaat atctgctcga tacgcacgag catttcgacc acacctcggc
      481 aaatttttat ttcgcccggc gcggcatacc cattgttgcg agttcgcgat gcgccggcgc
      541 gatgcgcgag caaggcggac ccgacttcga gcgcatgatg aaaccggtcc ctgagttata
      601 cgagcgtttt cctggacttt gtctcacact gcctgatgtg cagttcaccg ataggaccca
      661 ggtgacactc cccggagtga ctttgcacct taagtatcgc gccgagaacg gccatagcca
      721 cagcaagggc gacaccacga tctacttcga agaggaagaa gtcttcatcg ccggcgatct
      781 gctttacact gaagttcatc cggtgacgtt cttcggcaac attcccaact ggctgagctc
      841 tctcaaaccg ctattcgaca gtcattatca gcaactcgtc cccggccatg gcccagccgt
      901 agacggtgag caaaccgggc gcgcctactt caaacaaatg tacgactatc tggaagattt
      961 ttacggccat cttcaggaac tcgaagccgg ccgtgagagc agcgatcacg tcgctcaaca
     1021 catgctcagc ggcgccgatg ccaagctcgg caagacccga atggtagagc gtaatatcaa
     1081 tcaatttttg accggaaaat ggttttagat gaagccttgc ttctgcagag taccgagtcc
     1141 tccggcgtgc tccctgtcat gtcaggcgcc aacaactccg gccgtccctc gtcacgtatc
     1201 cctcgatacg tgctttgcac tactcgggaa accggttcat ccgacggtcc tgagtaggca
     1261 ccgagcagcg ctgccgtatc gaaggacaaa cccggagttt tcacataacc tggcggagcg
     1321 tatagccttg atcgttatta tcgcttgtgt tttctggctc agcacccatg ggcatgctgc
     1381 ggataagctc gaaaggcttc gcgctgccta ccctgcggtc gcaccgggtt cgaccccctc
     1441 ttggatcacc gccgagaaga aaatctggtc gaaatacggc tatgacgtcg aaactattct
     1501 ggtgagcggc ggtgcgcgcg ccgtgccagc gttggtcagc ggcagcgtgc agttcctttt
     1561 tggctcggat acgggcatca ccacggcgca gctgcagggg attccggtgg tcaggctcgg
     1621 cgtgacgatg aatacgctgg gctcctcact gatcacgcag cccggcattc agtccgtgca
     1681 ggagcttaag ggcaaggttc ttgggatctc gcgcgggcgc gatgcgagct acgtgcgtct
     1741 cgccaagctc ctccgtgaca acggactcaa tcccaatgat gatgtaaaat tcctttccat
     1801 cggtggcggc gaagccggcc gtctttctgc cgtcaaagcg ggcgtgatcc acggcacgat
     1861 gctgtttccg ccgctcgacc tgatcgccag aaacgacggt ttgaaagtcc tcgccaaatt
     1921 tgacgtgccg acacccggcg gtggcatcaa cacgacggcg acgctgctca aacaaaatcg
     1981 ctccatggtg gtcaatttcc tcaagggcta catcgaaggc attcactaca tgaaaacgca
     2041 caaagccgaa agtttgcatg tgctccaaaa atattttcag aactcggaca tgaccgcaat
     2101 ggcctatctt tatgacgaga ccacggggag gctagagaag gatctacgcg ccagcccgga
     2161 gtcgatccgt ttccatcttg aaatggccgg cctcgacgat cctcgcgcag ctaagctcag
     2221 tgaaaaggaa ttttgggatc cgagcgtcgt tgaagaaatc cgccgctccg gtttc
//
LOCUS       DASULZ010000720         2433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1095724, whole genome shotgun sequence.
ACCESSION   DASULZ010000720 DASULZ010000000
VERSION     DASULZ010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095724"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VFY96_14875"
     CDS             complement(<1..377)
                     /locus_tag="VFY96_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5607806.1"
                     /translation="MLTREENELLTQVGRGTPCGELLRRYWHPVAAAAELTGDKPIRP
                     VKILGEELVVYQDKRGNYGLVGEHCPHRLASLAYGRVDEEGIRCPYHGWKFEASGRCL
                     EQPAEPPGSTFKDRIKHVAYPVQ"
     gene            complement(416..1078)
                     /locus_tag="VFY96_14880"
     CDS             complement(416..1078)
                     /locus_tag="VFY96_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEX5607807.1"
                     /translation="MAREPIVERLVKLDTCAVSDALDSLGLKGATWGVRPLWECPRIA
                     GRAVTMKLKPAGLQQPTQHLGTAPIEAAETGDIIVIDNGGQLQFSCWGGLLALSAKLK
                     GLSGVVIDGASRDIDEARELKFPVYARGAVPMTARGRVIQDSYNQEIQFAGVQCHPGD
                     LVIADGSGVVIVVKEKEDEVVAAAEEIYRKEQEMADGIRKGYSGLEMLEKLGYEQMLK
                     KK"
     gene            complement(1140..2096)
                     /locus_tag="VFY96_14885"
     CDS             complement(1140..2096)
                     /locus_tag="VFY96_14885"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol ring-cleavage dioxygenase"
                     /protein_id="HEX5607808.1"
                     /translation="MAEVVIGIGTSHSPQLSIRASDWDHLLKKDQTDPRLDYTSLLKR
                     AKPGLETELTPEKFRQRDEACLKAVEDLGIALKTSRADVVVVFGDDQQEQFHDENMPT
                     FAIYHGKTLPVVKDNKHRPAGWKDAERRGWAETADQYETAHELANHLIRALVDAEFDV
                     ARCNKLREEIGVGHAFSFLYRRILPSGKLPMVPVMVNTYYPPNQPTPKRCYAFGRAVR
                     KAIESWDSGQKVAIMASGGLSHVVIDEEIDAMTIDGLKNKKPETLFRLPRERLRGGTS
                     EILNWVALAGAMEDRELKYFEYVTTYRSPAATGCGMAFAYWM"
BASE COUNT          473 a          750 c          629 g          581 t
ORIGIN      
        1 tattgaacgg gatacgccac gtgtttgatt cgatctttga acgtgctgcc tggcggctct
       61 gccggttgtt cgagacatcg ccccgacgcc tcaaatttcc agccatgata aggacagcga
      121 attccctcct cgtcgacgcg cccgtaggct agtgatgcca agcgatgcgg gcaatgctcg
      181 cccaccaaac cgtaattgcc tcgcttgtct tgatagacta ccaactcctc gccaagaatt
      241 ttcaccggac gaatcggctt gtccccggtc aattccgcgg cagccgccac ggggtgccaa
      301 taccggcgca gcagctcgcc gcagggagtg ccacggccga cctgagtcag cagttcattt
      361 tcttcacggg tcagcataga ttagatttgg tcacagggcg cagcatgctg cgcccctact
      421 tttttttcag catttgctcg tagccgagct tttccaacat ttccaatccc gaatagccct
      481 tgcggattcc atccgccatc tcttgctctt tacgatagat ctcttcggct gcggcgacca
      541 cttcatcttc tttctcttta acgacaatca ccacgccgct gccgtcggca atgacgagat
      601 cgcctggatg gcattgcacg ccagcgaact ggatttcctg attgtacgaa tcctggatca
      661 cgcggccgcg ggcggtcatc ggcaccgcgc cgcgcgcata aacgggaaat ttaagctcgc
      721 gcgcctcgtc gatgtcacgt gaagcgccgt cgatgacgac tccgctcaac ccttttagtt
      781 ttgccgaaag cgccaggagt ccgccccaac acgagaattg cagctggcca ccattgtcga
      841 tcacgataat gtcgcccgtt tccgccgctt cgatcggtgc ggtgcccaaa tgctgggtcg
      901 gctgctgcaa tccggcgggt ttgagtttca tcgtcacggc tcggccggcg atgcgcggac
      961 actcccagag tgggcgcaca ccccaggtcg cgcctttcaa gcccaaacta tcgagcgcgt
     1021 ccgaaaccgc gcaggtatcg agtttgacta accgttcaac aatcggttcc cgtgccataa
     1081 ccgaattcgg gcggacactt agatccgccc ctactctgtg atctgcgtta tttgtttccc
     1141 tacatccagt acgcgaacgc catgccgcag ccggtcgccg cgggcgatcg atacgtggtc
     1201 acgtattcaa aatatttcaa ttcccggtct tccatcgcgc cggcgagcgc cacccagttg
     1261 agaatttccg aggtaccgcc gcgcaatcgc tcgcgcggca atctgaatag tgtctccggc
     1321 tttttatttt tcaatccgtc gatagtcatg gcgtcgatct cttcatcgat caccacatga
     1381 ctcaatccgc cagatgccat gatcgcgact ttttgcccgc tgtcccacga ttcgatcgcc
     1441 ttccgtaccg cccgaccgaa tgcgtagcag cgcttcggcg tcggttgatt gggcgggtag
     1501 taagtattca ccatcaccgg caccatcggc agtttaccgc tcggcagaat gcggcgatag
     1561 agaaagctga acgcgtgacc aacgccgatc tcttcacgga gcttattgca gcgcgcgaca
     1621 tcgaactcgg catcgacgag ggcacggatc aaatgattcg ccagctcatg agcggtttcg
     1681 tactgatcgg ctgtctccgc ccaaccgcgc cgctccgcat ctttccatcc cgccggtcga
     1741 tgcttgttat ctttgactac cggcagagtt ttgccgtgat agatcgcgaa ggtcggcatg
     1801 ttctcgtcat gaaattgttc ctgctgatcg tctccgaaaa caaccaccac atcggcacga
     1861 gaggttttca gggcaatgcc caaatcttcg actgccttga ggcaggcctc gtcgcgttgg
     1921 cgaaactttt ccggcgtcag ctccgtttcc aaacccggct tcgctcgctt gagcaggctt
     1981 gtataatcga gccgcggatc agtttggtct tttttgagta gatggtccca gtccgaagcg
     2041 cgaatgctta actgcgggct gtgcgatgtg ccgattccga ttacgacttc agccatattt
     2101 cgtccccttg ctcttttcgt tctcagacga cgttgagaag gatcgtccgc tcgaatttct
     2161 atcgagaaca gatcgacggt gtcaagttgg cggcgaaaag ggcgaccttg gttcggatcg
     2221 cccggcgtga cgctgaattg ttgttgcgta aagcaagtca gcgcttcttt ggcaccagaa
     2281 tatctttcag ctgcgccacc atcgccggct tcaattcgta gagcccagcg agcgtcttgg
     2341 caatcgtctg cccgtcgacc ggatcgacat cgagctgaga tttcttcgcc tcggccatca
     2401 acccggggtc cttgaacgtc cgcatgaagg ctt
//
LOCUS       DASULZ010000721         3481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_691313, whole genome shotgun sequence.
ACCESSION   DASULZ010000721 DASULZ010000000
VERSION     DASULZ010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691313"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(374..>3481)
                     /locus_tag="VFY96_14890"
     CDS             complement(374..>3481)
                     /locus_tag="VFY96_14890"
                     /inference="COORDINATES: protein motif:HMM:NF017515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HEX5607809.1"
                     /translation="LCQGISVVTPHGGVERLPHLGASLANLAQARGVSEVIVAEMGAQ
                     PCAARLARRWGAKYVFIHNSEVFERARALNTGAALAEFDLVLWKDNDLLLPESFAEIA
                     AAELRSRNLDHLLPYEAIRYLNAADSRSVIAGTVAPATCKPARVLYGGRDVSGGAALV
                     RSDFLFRFGGIPDMFRGWGGEDNAWMHKASLFGRTGVTQRSGQYLFHLHHADSGAFAG
                     RPLSTNPYYSENVAMLGRLRAIASKEEFLQRFPDSSRRICPWDKSRRIALLYAPSGSA
                     AAASVERGLRDIYGIGCAAQPLSHDWILECADAEAIIAVDPPEHFLREADAEGLWPRI
                     LMLVTDSGAIVASRRALLERARAAVVLDTAMKPGEGAWAISAAKLNDPIGLAKVLVQP
                     LSLLMAGDKTRARQSADNLAGGELPVWVYWEGECPEWIRLCQQTIRCHAPGLRLLDRA
                     AFETLWDIDRDIHLERLQTAHRSDFVRAFLLARFGGLWIDSDCIVMQPLTEILASLRE
                     TDFVAHRDRQGFYPNGFIGARPNSIIASELYQRVCERLRAGRPLGWISLGGEPLTEVL
                     RTTTIKWRELPCETIQPVCWSQPEVFFEIGSPDEHELKRDPSALCYMLSNTEVQKFVA
                     KNPERKILADGTFFRYLIKRSLETDAEDARVSMVTSPLRIAREVAALLETISKLQPRR
                     ILEIGVGTGIRGLLIRESYGDRESDIFELAGVTSNGAVKLTVREDVYSSILQEPLDRI
                     HLLDSKLWDLVILGSGWEAATELAIQFLKKALEKSAYVLLFDSDVSPELTASLVAFSD
                     DLVCAMPADEPQAAGPRHFLFSRNDSKRIARRHRIGRIFEQIFRDNCRRRDESVSGPG
                     SCLAQTYEIRQRLPFLFADLGIETLLDAGCGDFHWLQHVDLGLKEYIGIDIVGDLIAR
                     NRQQFGLPNRRFILADLTEAPLPRSDFILCRDCLVHFSFADIVRILRNFGRSGSMYLL
                     TTTFPERAENQEIATGDWRTLNLERAPFHFPAPMRIINERCTERGGIYADKSLGLWRL
                     SDICL"
BASE COUNT          803 a          993 c          953 g          732 t
ORIGIN      
        1 acgttgtggc attccacaag tatttttttc ttcgtcgatg gcggcgcctt ttcttcgcta
       61 ttttcgcggg gtgcttctgt cggcaccgca gccagctaag gggctaattc gtggtccgat
      121 gcgcttacag cgaatgtaaa acttccgtac gctgatatgt gcatgatcag gatggccgat
      181 ctattcgcac gaggagcgcc gccggacggc cggccgcggg cggtgtcttg ttcactcaga
      241 cttcgcttgt tcatctgctg ttgtaagtgc cgtcggcgta ctggagcgcc agatcttgcc
      301 ggagccgtgt cttcgagttt ctctcgttgc ggcccatccc tcatttcgtg agacaacagt
      361 ttacacatcg tttttagagg caaatatcgg acaagcgcca gagacctaag cttttatcgg
      421 catagatccc gcctctctca gtacaccgct cattgataat tcgcatcgga gccgggaaat
      481 gaaacggtgc ccgctcaaga ttaagggtgc gccaatctcc tgtcgcaatc tcctgattct
      541 cggcacgctc cggaaatgtc gtcgtgagca gatacatgga gccgcttcgg ccgaagtttc
      601 gcaaaattcg aacgatatca gcaaaagaaa aatgcacgag acagtcccga caaagaataa
      661 aatcgctgcg ggggaggggc gcttcggtca aatcggccaa aatgaaccga cgattcggca
      721 gaccgaattg ttgtcgattg cgagcgatca gatctccaac gatatcgata ccgatgtact
      781 cttttaagcc caggtcgacg tgctgcagcc agtggaaatc accacatcct gcgtcaagca
      841 aggtttcgat gccgagatcg gcgaaaagaa acggcaaccg ctggcggatt tcgtaagtct
      901 gggcgaggca ggagccaggg cccgaaacgg actcatcgcg acgccggcag ttatcgcgga
      961 aaatctgctc aaagattcgc ccgatcctgt ggcgccgcgc aatacgcttc gaatcgttac
     1021 gggagaacaa gaagtgacgc gggccggctg cctggggttc atcggccggc atggcgcaaa
     1081 ccagatcgtc gctaaatgcc accagcgacg cagtcaattc aggagaaacg tctgagtcga
     1141 agagcaatac gtaagcgctt ttctccagcg ctttcttcag gaattgaatc gcaagctcag
     1201 tcgcagcttc ccatcccgac ccgagtatta ccaggtccca taatttcgaa tcgaggagat
     1261 gaattcggtc cagcggctcc tgtaggatac tgctataaac gtcttcgcgc acggtgagct
     1321 ttacggcgcc attcgacgta actcccgcga gctcaaaaat atccgattct ctgtctccat
     1381 aactctcgcg gatcagcaac ccccgaattc ctgtaccgac accaatctcg aggatgcgcc
     1441 gcggctggag cttggagatc gtttctaaga gtgcggcaac ttcgcgagcg atccttaagg
     1501 gcgaagttac catggacaca cgggcgtctt cggcgtcagt ctccaggctg cgtttgatca
     1561 agtagcgaaa gaacgttccg tcggcgagta ttttccgttc aggatttttc gcgacgaact
     1621 tctgtacttc cgtgttggac aacatgtagc acagcgcaga tgggtcgcgc ttaagttcat
     1681 gttcgtcggg cgagccgatt tcaaagaaca cttcgggctg actccaacaa accggctgga
     1741 tcgtttcgca gggaagctca cgccacttta tggtggtcgt gcgcagcact tcagttaacg
     1801 gctcgccgcc taaggaaatc catcccagcg gtcgcccagc gcgcaatcgt tcgcaaactc
     1861 gctggtataa ctcggaagca atgatgctat tggggcgcgc tccgatgaag ccattcggat
     1921 agaacccttg acggtcccgg tgagctacaa aatcggtctc gcgtaacgaa gccaatatct
     1981 cggttaacgg ctgcatcaca atgcagtcgg agtcgatcca caaaccgcca aaacgtgcaa
     2041 gtaagaacgc gcgcacaaag tcggaacgat gagctgtctg caggcgctcc agatggatat
     2101 cacgatcgat gtcccaaaga gtttcgaaag ccgcgcgatc gagcaaacgg agaccaggcg
     2161 catgacagcg aatagtctgt tgacataaac gtatccactc cgggcactca ccttcccaat
     2221 agacccacac cggaagctca ccgcctgcga gattgtcggc gctttgccgg gcacgagttt
     2281 tatccccggc catcagcaac gacagtggtt gaacgagaac cttcgccaat ccgatcgggt
     2341 cgttaagctt tgcggcggag atcgcccaag ctccttctcc tggtttcatc gctgtgtcca
     2401 gcacaaccgc agcgcgtgcc cgctccagca atgcccgtcg cgaggcgacg attgctccgg
     2461 aatcagtgac aagcatcaga atgcgcggcc aaagtccttc tgcatcagct tctcttagaa
     2521 aatgctcggg agggtcgacc gcgatgatcg cttcagcatc agcgcattca agaatccagt
     2581 catgcgataa tggctgtgct gcacagccga ttccataaat gtcgcgtaag ccgcgctcca
     2641 cgcttgccgc agcggcgctg ccagatgggg catacagcaa ggctatgcgc cgcgacttgt
     2701 cccagggaca aatccgtcgg cttgaatcgg gaaatcgctg gagaaattct tccttgctag
     2761 ctatggctcg taacctgccg agcattgcaa cattttccga gtaatacggg ttggtgctca
     2821 aaggtctgcc ggcaaatgcg ccggaatcgg catgatgcaa atggaacaga tactgcccgc
     2881 tgcgctgggt cacgccggtt cggccaaata acgaagcttt gtgcatccac gcattgtctt
     2941 cgccacccca gccgcgaaac atgtcgggaa taccgccaaa acggaatagg aaatccgatc
     3001 tcaccagggc ggcgccgccg ctcacatcac gtccgccata gagcacccgg gcgggtttac
     3061 aagtcgcggg agcaacggtt cctgcgatca cactccggct gtcagcggcg ttgaggtagc
     3121 gtatcgcttc atagggcagt agatgatcca gattgcggct gcgcagttcc gccgcggcaa
     3181 tctcggcaaa agactccggt agtaagaggt cattgtcttt ccataacacc agatcaaact
     3241 ccgccagtgc ggcgcccgtg tttagagcgc gggcgcgctc gaaaacctcg gaattgtgga
     3301 tgaagacata tttagctccc cagcggcgcg cgagacgcgc agcacaaggt tgcgcgccca
     3361 tctcggctac gatcacttcg gaaacacccc tggcttgggc aaggttggct aatgacgcgc
     3421 cgagatgcgg caatcgttct acgccgccgt ggggggtgac gacactgatc ccctggcaga
     3481 g
//
LOCUS       DASULZ010000722         8712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_590410, whole genome shotgun sequence.
ACCESSION   DASULZ010000722 DASULZ010000000
VERSION     DASULZ010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8712
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590410"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VFY96_14895"
     CDS             <1..792
                     /locus_tag="VFY96_14895"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5607810.1"
                     /translation="AVPPAMHHYYMAQAYLVSLARGRATKEIDEAIRLDPKNPKFHLL
                     KMRIFLEQDKSVEGSKEALAALQSGVDYIPDVLAMSDEFYLPEAQLVYRKVIEMGSKE
                     VLPYLGLGNIALHSGDLPEAEKWFVQARDLQPDHPAVLLAWGRLTAAKAQQLNDEAQM
                     KKQLQQARGLLEKSKSKGEESATIHTELAAIYYKLGVWEKAAAEYAEALRMRRRRNDL
                     RFSLGQAYAQLGRVGDAEQKYREILSLSPDDADALKALQDLGKRY"
     gene            927..1742
                     /gene="proC"
                     /locus_tag="VFY96_14900"
     CDS             927..1742
                     /gene="proC"
                     /locus_tag="VFY96_14900"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX5607811.1"
                     /translation="MSSDKIGVIGAGKIGAAMTRGIIRGGLASKENVMASDVSDALRE
                     SIVQDLGIRATPDNTEVCSFADVVILAVKPQIVDPVARQIAKELGKTKLLVSVAAGVP
                     LRRLETQLQPGARVVRVMPNICCVVGAGAAGYAPGAHATEQDMERVGAILNSFGIGMP
                     VEEKYLDAVTGLSGSGPAYVFLFMEALADGGVQVGLSREVAVRLALQTVYGAARMALE
                     HKTHLAELRDEVASPGGTTIAGLYAMEQNGFRGTIMDAVVKATQRSQELGQSN"
     gene            complement(1802..2326)
                     /locus_tag="VFY96_14905"
     CDS             complement(1802..2326)
                     /locus_tag="VFY96_14905"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX5607812.1"
                     /translation="MIRSKWLRLLTIGAFLLAANALIALSLEAAAVSGTYITTADVSL
                     RSGPGVSYPVVTTLPKGIKINVVGRQGYWLKVESKHGDAPGYIDEQFAAPEGSTETST
                     TKPPQLTGAYRTLVDVDLRQGPGAKYPFVARLPSGIKVNVVRAEGDWLRVESKHGGKP
                     GYLERRFVERWTEN"
     gene            2521..4344
                     /gene="bamA"
                     /locus_tag="VFY96_14910"
     CDS             2521..4344
                     /gene="bamA"
                     /locus_tag="VFY96_14910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEX5607813.1"
                     /translation="MRPLSKTVIALLISLMLFPDRASAVLVDALDPARQWKVEGIEFS
                     GNKAVSADELSEAMITKARAWFRIWEDRPPFDPITFQTDLERLLRFYETKGYYGTTVA
                     YDLDVDESRSQVTARITIQEVPPVLISEIDVEVEGDGNARTALPKELPVKRDAIFREA
                     EYQQAEQILRSAFLDHGYAHVKTERKAQVNVDERRVSIQYSVEPGPVAVFGATAVKGT
                     DTVDPDIITRELTYHAGETYSQSKISESRDKLLALDLFGSVRIAPAETQGMPAIVPIE
                     IEVTEKSHREVRLGVGWGTEDHFRTQLEWRHLNWLGGGRRLSILGKYSSIAIRGAIDL
                     VQPHFLTPRTEGAVNWSYDQEDEDTYLRHVGRFAPRLEHRFSPALTGFVLYRFEFDKL
                     NEIDDATVSALGEIRRKGFLSGPSAGIVWSTIDNPLNPKKGEVVTFGFDYAGWGGDFN
                     FYKMAAEGKKYIDIGWQTIFASRLKIGLADSIGASKNLPLFERFYSGGERSVRGYIRR
                     HLGPLSSINDPLGGLSLVEGSLELRRPIWKELSGALFVDFGQVSKQSFDLPFDDLQFS
                     RGFGVNYTTPVGPLSFYVGFPVHPPRGDSSWQIHFSIGAFF"
     gene            4349..8152
                     /locus_tag="VFY96_14915"
     CDS             4349..8152
                     /locus_tag="VFY96_14915"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEX5607814.1"
                     /translation="MRKILRVILWLGAGFLVLLLLAVIGVTIYTRTERFTRWARDEGV
                     AAVNNLIQGSLTVERLEGSVWRHLVLHNVGLRYQDKEILIVPRLEVSFSLLPLIWGEL
                     RISSIDADGPRATLLQDQEGRWNVVEAFLPRHPEPEKKSEFVALVDRFRLVNAAIHLR
                     PTANDSTPYDLTDLNLQGRMGIRPAGLILNVDEVTTQLATPGKPELRLKGGLEYTQAE
                     TPGSTFKVKDLWAVSRNSRIKINGEVTPAETLKVKVDALVDRLAPADIAYFVPQWPLK
                     PVLAGNVSVAGPLDDLNGAVQLASVGAKIAGKFRADVTRETILYSATATVSGFDLRQW
                     LNNPSLAGVLGGTVEAAGKGFALGDIAGTTRLQVRSAETEGWKLGDVNIEGRLQKSIA
                     SIDGRLNSTTGGANWSGKINLADKRPSYDLQIAVKDFDLQQVGQSSKALNGKLNFRGT
                     VTGSGISLADMNAHADVQVLPSSVASIAVNEGNFNLNLNGNKVRIARAVLSTADSTVS
                     AAGEFGLDGEMSGSLDYRARIGNVAPWLALVSRKGSGSMDLAGRAQGSLSDLQTRGTA
                     RLSALQVDGMGLKNGSIGYSLRGSKEQFFPEGNITATLIDLDAGLTLRRIEANAKLSR
                     QLQSIDLTLTAQDSQDRKHNVAGIFNFSPDALIVKLNQASLAAPDGIWKLFRPATVMK
                     HNDSISIDQFALRNGERQVSISGSFAFSGGQDLNVNVDRLPVETLTAFLSEPPKMSGL
                     LALRARVTGTAAAPELTASARLSNSTVAGQSYDGADADMSYKDKQASVRLAVRQDSTH
                     TLTGTGNVPLNLSWQNGWRAEFGDGLDARAQSAGLSIAFLNAFSGKTAENISGDLSLD
                     VTARGSIKAPNLGGTFHLRNGKLRVIPAGVDVNQVAMDGRLDSSTITIGEFIARAQDG
                     EIRGNGALPLRDYDRAAVKLLLSAKNWPAIQTARYQAKIAGNVAVQGALLAPRVTGQL
                     TVLEGSLRPDLAFLEQSKVPLQRDETILVVDNSAGREPEQRLDQEAAPSSNNLLFKKL
                     SVDLTVRVPGNLWIRHPSMVSEISGNVRATKEPDAEVDLTGRIDIVRGSLVFQGRRFQ
                     LTRGSVQFTGGGKINPTLDIAAQYKLPEYDVEVVLTGTAQKPSLTLSSQPRLDQADIL
                     AVLLFGRPINSLNQNQRGSVQQSALNIASGYVAGKLASSVTQALGLDTLGLDVGDLDT
                     SGGRIGFGHYVGNKTYFSVGQELSGEHGQDVSLEYQIAPNWKIGTSRSTTGSNAIDII
                     WQKRY"
     gene            8349..>8712
                     /gene="mscL"
                     /locus_tag="VFY96_14920"
     CDS             8349..>8712
                     /gene="mscL"
                     /locus_tag="VFY96_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX5607815.1"
                     /translation="MFAEFRQFIRRGNVIDLAVAVVIGVAFGKIVTSFVDDILMPPIG
                     LALGRVDFSNLFINLSGNEYPSVAAAKAAGAATLNYGIFINNILNFIIIAFAVFLLVR
                     QINRMQTPAPAPAAATKDC"
BASE COUNT         1956 a         2333 c         2530 g         1893 t
ORIGIN      
        1 gcggtgccgc cagcgatgca ccattattat atggcccagg cctatcttgt gagcttggcg
       61 cgcggacgtg cgaccaagga gatcgatgaa gcgatccggc tcgatccgaa gaatccgaag
      121 tttcaccttt taaagatgag aatctttctc gaacaggata agagcgtgga aggatcgaag
      181 gaagcgctcg ctgcgctgca gagcggcgtt gattacattc cggatgttct cgccatgagc
      241 gacgaattct acttgccgga agcccagctt gtttaccgaa aggtcatcga gatgggaagc
      301 aaagaggtct tgccctacct cggtttggga aatatcgcgc tccattcagg cgatttgcca
      361 gaggcggaga aatggttcgt tcaggcgcgc gatttacaac ccgatcaccc ggcggttctg
      421 ctcgcctggg gccgtttaac ggcagccaag gcgcaacagc tcaacgacga ggcgcagatg
      481 aaaaaacagc tgcaacaagc gcgcgggctg ttggaaaaat ccaagtccaa gggtgaggag
      541 tcggcgacga ttcatactga attggccgcc atctattaca agctcggcgt atgggagaag
      601 gcggccgcgg aatatgccga ggccttacgc atgcggcgca ggcgtaacga tttacgcttt
      661 tctttggggc aggcctacgc ccagctcggt cgggtcggag atgccgagca aaagtatcgg
      721 gaaatcctct cgctctcgcc cgatgatgct gatgcgttga aagcgcttca ggatctcggc
      781 aagagatatt gagcgaatat tcatgttggc aggaagcctc cggcggaaag aggaggcttt
      841 ttagttgacg cagtaagacg actttgaact tctagggacg aggaattatg ctggcgcgcg
      901 gatgataacg ggcgtaggag gtttccgtgt cgagtgacaa aatcggcgtg attggagcag
      961 gaaaaattgg cgcggccatg acgcgcggaa ttattcgagg cgggctggcg agcaaggaaa
     1021 acgtgatggc cagcgacgtc agcgacgcgc tccgggaatc gattgtgcag gatctcggca
     1081 tcagggcgac gccggacaat accgaggtgt gctcctttgc cgacgtcgtg atcctcgcgg
     1141 tgaaaccgca gatcgtcgat ccagtggcac gacagattgc aaaagaactc ggcaagacca
     1201 agcttttggt gtcggtcgcc gcgggagtgc cgttgcgacg gctcgaaaca cagctacagc
     1261 caggcgcgcg ggttgtgcga gtcatgccca atatttgctg cgtggttggc gccggcgcgg
     1321 ccggctatgc ccccggagcg catgcgacgg aacaggacat ggaaagagtc ggcgcgattc
     1381 tcaacagctt cggaatcggt atgccggtgg aggaaaaata tttggacgcg gtcaccggtc
     1441 tgagcggcag cggacccgcg tacgtatttc tgtttatgga agctttggcc gatggcggcg
     1501 ttcaagtcgg tttgtcgcgc gaagtggccg tgcgactggc gctgcaaacg gtctatggcg
     1561 ccgcgcgcat ggcgcttgag cacaaaacgc atttagctga gttaagggac gaagtggcgt
     1621 caccgggagg gacgaccatt gccggcctgt acgctatgga acaaaacggc tttcgcggca
     1681 cgattatgga cgcggtggtg aaagcgacgc agcgatcgca ggagctagga caaagcaatt
     1741 aacagaaccg ttcaaacgtc tgcttcgcgg cgtacctccc caacccgtcg tcggaaaagc
     1801 actaattttc ggtccagcgc tcgacgaatc tcctctcgag atagcctggt ttgccgccgt
     1861 gcttggactc gacgcggagc caatcgccct cggcccgaac cacgtttact ttgatgccgg
     1921 acggaagtcg cgccacaaac gggtacttcg ctccaggtcc ctgtctaaga tccacatcca
     1981 ctagggtgcg gtaagcgcct gtaagctgcg gtggcttcgt cgtgctggtt tccgtgctac
     2041 cttcgggggc ggcgaactgc tcatcgatat agccgggcgc atcaccatgc tttgactcca
     2101 ctttgagcca gtaaccttgt cgaccgacaa cattgatttt gattcctttc ggcaaagtgg
     2161 taaccaccgg gtagctcacc cctgggcctg accgcaacga cacatcagcg gtcgtgatgt
     2221 aggttccact gaccgcggcc gcctcgagtg acagggcaat gagcgcgttc gcggccaaga
     2281 gaaaagcgcc gatggtcaaa agtctaagcc attttgatcg gatcacggct gtgtatcggc
     2341 tgttcgacac gaacaacatg ttttccgaaa taaacttagc ctttattgct ctctcggtcc
     2401 accgatccgc agccatccgg cgtaatcgag catgcatttg gttcaaccct attcagcacg
     2461 gcatcaagaa atcggccaca gcgtattccg gcatttctaa cgaggtgtta agaaggtgtg
     2521 gtgaggccgc tcagcaaaac cgtcatcgcc ttgcttataa gtttaatgct ttttcccgac
     2581 cgcgcctccg cggtcttggt tgatgctctc gatcctgcgc gccaatggaa agttgagggg
     2641 atcgaattct ctggcaacaa ggcagtttca gccgacgaac tcagcgaggc gatgatcacc
     2701 aaagcgcgcg cttggtttcg catttgggaa gatcggccgc cgttcgaccc gatcacgttc
     2761 cagaccgatc ttgaaagatt attgcggttc tatgagacga agggctatta cggaacgacg
     2821 gtggcttacg atcttgatgt cgacgagtca cgctcgcaag ttacagcgcg gattacgatt
     2881 caggaagtcc cgcccgtatt aatctcggag atcgatgtcg aggtcgaggg cgatgggaac
     2941 gcgcgaacgg cgcttccgaa agaactgccc gtgaagcgag acgcgatctt tcgcgaggcc
     3001 gaatatcagc aggccgaaca gattctgcga agcgcttttt tggaccatgg ctatgcgcac
     3061 gtaaagaccg agcggaaggc gcaagtcaat gttgatgaac gtcgcgttag cattcagtac
     3121 agcgttgagc ccggacccgt cgcggttttc ggcgcgacag cggtgaaggg aaccgacacg
     3181 gtcgatcctg acattattac ccgcgaactc acgtatcatg cgggagaaac ttattcccaa
     3241 tcgaaaattt ccgaatcgcg cgacaagtta ctcgctttgg accttttcgg ctcggtgcga
     3301 atcgctcccg ccgaaactca gggcatgcct gcgatcgtgc cgatagaaat cgaagtcacg
     3361 gaaaaatcgc accgtgaagt gaggctcggc gtcggttggg gcacggaaga tcacttccgc
     3421 acgcagttgg aatggcgtca cctcaattgg ttaggcggtg gtcggcgttt atccattctc
     3481 ggcaagtatt catcgattgc gatcagagga gcgatcgatc tggtgcaacc tcattttctg
     3541 acgccgcgca cagaaggagc agtaaactgg agctacgacc aggaggatga agacacttat
     3601 ctgcgccatg tcggccgctt cgcaccgcgc ctcgagcacc ggttttcacc ggcgctcacg
     3661 ggatttgtct tgtatcgctt tgaatttgac aagctcaacg aaatcgatga tgcgacggtc
     3721 agcgctctcg gggaaattcg gcgtaaagga tttctttcgg gcccgagtgc cggcattgtc
     3781 tggagtacca tagacaatcc gctaaacccg aaaaaaggcg aggttgtcac attcgggttc
     3841 gactatgccg gctggggcgg tgatttcaat ttttataaaa tggccgccga gggcaaaaaa
     3901 tatatcgata tcggctggca gacgattttt gccagccgtc tcaagattgg cttggccgac
     3961 tctatcggtg cgagcaaaaa tttgccgcta tttgaacggt tttattccgg cggtgaaaga
     4021 agcgtccgtg gctacattcg tcgtcatctc ggaccgctga gctctatcaa tgatccgctc
     4081 ggcggattga gcctcgtcga aggttcgctt gaactgcgcc gcccgatctg gaaagaatta
     4141 agtggagcgt tgttcgtcga tttcggccag gtttcgaaac agtcattcga ccttcctttc
     4201 gacgatttgc agttctcgcg cggtttcgga gtgaactaca cgactccggt cggacctctc
     4261 agtttttacg tcggctttcc agtccatccg ccgcgcggcg acagttcgtg gcagattcac
     4321 ttcagcatcg gcgcgttttt ttaaaactat gagaaaaatc ctacgtgtga tcctttggct
     4381 gggtgcgggg ttcctagttc tgctcctgct cgcggttatc ggcgtgacga tttatacccg
     4441 aaccgaacgc tttacccgct gggcgcgtga cgagggggtc gctgcggtca acaacttaat
     4501 acagggctca cttactgtgg agcggcttga aggatcggta tggcgccacc tagtgcttca
     4561 caacgtgggg cttcgctacc aagacaagga aattcttatc gttcctcgtt tggaagtttc
     4621 attctcattg ctgccgttga tttggggcga gctgaggatc tcctctattg acgccgacgg
     4681 gccgcgcgca actttgttac aggatcagga aggtcgctgg aacgttgtcg aagctttctt
     4741 gcctcgtcat cccgagccgg aaaaaaaatc cgaatttgtc gccctggtcg atcgctttcg
     4801 tttggtcaac gctgcgattc acctgcgccc aacagccaac gacagcacac cgtatgacct
     4861 cacggatcta aacctgcaag gccgaatggg tattcggccg gcgggcctca tcctaaatgt
     4921 tgacgaggtt actacgcaac tcgccacacc cggtaaaccc gagcttcgcc tgaaaggcgg
     4981 tctcgagtat acgcaagccg agacaccagg atcgacgttc aaggtgaaag atttgtgggc
     5041 cgtgagccga aactcgcgaa tcaaaatcaa cggcgaagtg acacctgccg agaccctcaa
     5101 ggtgaaagtg gacgctctcg tggatcgact cgccccggca gatatcgctt acttcgttcc
     5161 gcaatggccg ctcaaacccg ttctcgcggg caatgtttct gttgccggtc cactcgacga
     5221 tctgaacggc gctgtccaac tcgcgagcgt gggcgctaaa atcgctggaa agttccgcgc
     5281 cgacgtgacc cgagaaacta ttctttattc ggcaacggcg acggtgagcg gctttgatct
     5341 tcggcaatgg ctcaataatc cgagcttggc cggcgtgctc ggaggaacgg tcgaagccgc
     5401 cggtaaagga tttgcgctgg gcgacattgc ggggaccacg cgactgcaag ttcgctctgc
     5461 ggaaacggaa ggttggaagc tcggtgatgt caatatcgaa gggcggttgc agaagagcat
     5521 cgcgagcatc gatggtcggc tcaacagcac cacgggcggc gccaactggt cgggcaaaat
     5581 caatctggca gacaagcggc cgagctatga tttgcaaatt gcggtaaaag attttgacct
     5641 ccagcaagtt ggacagagta gtaaggcgct taacggcaag ctcaatttcc gcggcactgt
     5701 gacaggctcg ggaatcagtc tcgccgacat gaacgcccac gccgacgtgc aggttttgcc
     5761 ttcgtcggtg gcttccattg cagtcaatga gggaaatttc aatttgaatc tgaacggtaa
     5821 caaagtccgc attgcgcgcg ccgtgctcag cactgctgat tcaacggttt cggccgcggg
     5881 cgagtttggc ttggacggag aaatgagcgg cagcctcgat taccgggctc gcatcggaaa
     5941 tgtggcgccc tggctagcgc tcgtcagtcg caaaggttcc ggctccatgg accttgccgg
     6001 ccgggcacag gggagtctca gcgatttgca gacgcgcggc acggcgcgtc ttagcgcgct
     6061 ccaggtcgac ggaatgggac tgaaaaatgg ctctatcgga tactctttac ggggctcgaa
     6121 ggagcaattt tttccggagg gaaatatcac tgccactctt attgacctcg atgccggtct
     6181 gacattgcga cggatcgaag caaacgcgaa gttatcgcga caactgcagt cgattgatct
     6241 tacgttgact gcccaggata gccaagatcg caaacacaac gttgcaggaa tttttaactt
     6301 ctcccctgac gctttgatcg tgaagttgaa ccaggcatct ttggcggcgc ccgatggcat
     6361 atggaaactc ttccgcccgg cgacggtgat gaagcacaat gactcgatat cgattgacca
     6421 gttcgcgcta agaaacggtg aacgacaagt gtccatcagc ggtagcttcg cattcagcgg
     6481 cgggcaggat ttaaacgtaa acgtcgatcg cctgccggtg gaaaccctca cggcattttt
     6541 gtcagaaccg cccaagatgt cagggcttct tgcgctccgc gctcgggtca ctggaaccgc
     6601 tgctgctccg gagctcacgg cgtcagcccg actgagcaat agtacggtgg ctgggcaaag
     6661 ctatgacggt gcggatgccg atatgtcgta taaggataaa caagcgtctg ttcgccttgc
     6721 cgtacgacaa gactcgacgc acacgctcac cgggaccggc aacgttccgc tcaacctcag
     6781 ttggcagaac ggttggcgcg cggagtttgg cgatggtctt gacgcgcgcg cgcagagtgc
     6841 cggattgagc atcgcgttcc tgaacgcctt ctccgggaag accgcagaaa acatctcagg
     6901 cgatttgagc ctcgatgtca ccgcccgggg ctccatcaaa gcgcccaacc tgggcggcac
     6961 atttcatttg cgtaatggca agctccgagt gattcccgcc ggcgtagatg tgaatcaagt
     7021 cgcgatggac gggaggctgg attcgagcac catcaccatt ggcgaattta tcgcccgagc
     7081 gcaggacggc gagatccgcg gcaatggagc gcttccgctg agggattatg accgcgcggc
     7141 cgttaaactt ttgctaagcg ccaaaaactg gccggcgata caaacggcgc gctatcaagc
     7201 caaaatcgcc ggcaatgtcg ccgtccaagg cgctttactg gcgccgcgtg tgaccggcca
     7261 gcttacggtc ttggaaggtt cgctgcgtcc tgatctggct tttttggaac aaagtaaagt
     7321 gcctttgcaa cgtgacgaaa ccattctcgt tgtcgacaat tccgctggac gcgaaccgga
     7381 acagcgtctc gatcaggaag ccgccccctc aagcaacaat ctgttattca aaaagctcag
     7441 cgtggaccta acagtccggg tgccgggaaa tctctggatc cgacatccct caatggtttc
     7501 tgaaatctcg ggcaacgtgc gcgcgacaaa agaaccggat gccgaagtcg atctaaccgg
     7561 gcgcatcgat atcgtcagag gctcgctggt ttttcagggc cgaagatttc aattaacgag
     7621 aggctcagtc cagttcaccg gcggtggtaa aatcaatccc actctggata ttgcggcgca
     7681 gtataagttg ccggagtatg acgttgaggt cgttctcacc ggaaccgcgc aaaaaccgtc
     7741 attgacactt agtagccaac cgcgcttaga ccaagccgac attctcgcgg tattgctttt
     7801 cggccgtcct attaactccc tcaatcagaa ccagcgcggg tcagtgcagc agagcgcgct
     7861 caatatcgcg agcggttatg tggccggcaa gctcgctagt tcagttacgc aagcgctcgg
     7921 actcgatacg ctcggtttgg acgttggaga cctcgataca tctggcgggc ggattgggtt
     7981 cggccactac gtcggtaaca aaacctactt ctccgttggc caagagcttt cgggcgagca
     8041 tggacaggac gtttcgctgg agtatcaaat cgcgccgaac tggaaaatcg gcacctcccg
     8101 cagcaccacc ggttccaatg ccatcgatat tatctggcag aagcgttact gagcgaggcg
     8161 cctatcgaaa ggctatcgct cggcactgcg caacactcgg atcacaccgc aagcccaata
     8221 cgaacattag ttgacttaat ctttgtccgc cttataagaa gtgccaacgg ttgacaaaaa
     8281 cggcatctgc acgcgaccgg gcatcaccgg ccgttgaaaa ttgcgctagg cggaaggagg
     8341 acaaactcat gttcgctgag ttcaggcagt tcattcggag aggaaatgtg attgatctgg
     8401 ctgtggctgt ggttatcggc gtggcgtttg gcaagatcgt gacgtcgttt gttgatgata
     8461 ttctcatgcc gccgatcggt ttggcgctgg gtcgagtgga tttttcgaat ctattcatta
     8521 atctctccgg caacgaatat cccagtgtgg cggcggcaaa agcggccggc gcggcgactt
     8581 tgaactatgg cattttcatc aacaacattt tgaacttcat catcattgcc ttcgcggtat
     8641 tcctgctcgt caggcaaata aaccgaatgc aaactcccgc gccggcgcct gccgcggcga
     8701 caaaagactg cc
//
LOCUS       DASULZ010000723         3101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_488582, whole genome shotgun sequence.
ACCESSION   DASULZ010000723 DASULZ010000000
VERSION     DASULZ010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488582"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VFY96_14925"
     CDS             <1..513
                     /locus_tag="VFY96_14925"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX5607816.1"
                     /translation="EEAAAAGTLSLWLSGSKAIFDAHQPTFKAMGNKVLYVGELGNAK
                     LVKNAMAMFAAVTHLTLAEICSWLKKGGLTEDTFRTILKNSQQDSVAIDRIMEIMVSR
                     KYKPRKSWMPKDVGFGLDMAREMEVPMPFVALAYQMFSIAQATGMDGYEATGIACNVY
                     DAITGQNRGL"
     gene            519..1058
                     /locus_tag="VFY96_14930"
     CDS             519..1058
                     /locus_tag="VFY96_14930"
                     /inference="COORDINATES: protein motif:HMM:NF014001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HEX5607817.1"
                     /translation="MAGLAPERISRFLTYILRHRPQDYPLVFDEHGFVDWRDVVQLVQ
                     ERYYDVTEEQIRAVVVDAEKKRFELAGDKVRATYGHSFPIDFGQQSVQPPDSLYYGTA
                     RDLAQSMLRNGLKPRDRQYVHLSVSAEEAESVARRHDPTPAVIVVQAGAAHDEGVRFY
                     RSGPLFLTENVPARFLSLK"
     gene            1192..2913
                     /gene="argS"
                     /locus_tag="VFY96_14935"
     CDS             1192..2913
                     /gene="argS"
                     /locus_tag="VFY96_14935"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX5607818.1"
                     /translation="MAPLPGRAEWTTLVMRSQLREVLARAVEKTAKTEKLNAGELPPI
                     LVEPPKQREFGDLSSNVAMVWARRAKRPPRSIAEAVLKNLEDPDGILARQEIAGPGFL
                     NFSFAPKFFYREFRELVSGRNLQIDLGHGEKIQVEFASVNPTGPLHVGHGRVAVIGDA
                     LARLHQAAGFSVEREYYVNDAGNQMDNLGRSISARYRESFGEDVAFPENGYPGDYVKD
                     IAAEIRGQQGDKYLHDGEAAQKFFRETGGDLLLNMIRRQLTDFGITFDSFFSEKAMRE
                     RGEVAECLELLRQHRLLYFQDGAEWYKSTQFGDEKDRTVIKSDGELTYFSSDIAYHRN
                     KYQRGFQKLINVWGADHHGYVPRLKAAMQGLGYDPNRLHVVLVQMVQLTRGGEPVRMG
                     KRSGEFISLEEVLDEVGTDAARFFFLMRKSDSHLDFDLDLAKRQSSDNPVFYVQYAHA
                     RVASIFEQAARNGIILDQRDNVPIERLALAEELGLIRRMIEFNDVLEESVRELEPHRM
                     IFYLVDLASDFHHYYNHARVITDDADLSRARLLLVENVQKTVRRGLEILGIEAPTKMA
                     ARVEGDQ"
BASE COUNT          703 a          792 c          905 g          701 t
ORIGIN      
        1 gaggaagccg ccgcggccgg gacactgtcg ctttggcttt ccgggtccaa ggcaattttt
       61 gacgcgcacc aaccgacatt taaggccatg ggcaataagg tcctgtacgt cggtgagctg
      121 ggcaatgcca agttggttaa gaatgctatg gcgatgttcg cggcggtgac gcatttgacc
      181 ttggcggaaa tttgctcctg gctcaaaaag ggcggcttaa cggaggacac ctttcgcacg
      241 atattgaaaa actcccaaca agattcggtt gcgatcgacc gcatcatgga aatcatggtg
      301 agccgaaaat ataagccgcg caagtcctgg atgccgaaag acgttggctt cggtctggat
      361 atggcgaggg aaatggaagt gcccatgccg ttcgtcgccc tggcgtatca gatgttttcg
      421 atcgcgcagg caacaggcat ggacggctac gaagccactg ggatcgcgtg caatgtttac
      481 gacgcgatca ccgggcaaaa cagagggctc tagcgagaat ggcggggctc gcaccggaac
      541 gtatttcacg atttcttacc tacatcctgc gccaccggcc gcaggattat ccgctggttt
      601 ttgacgagca tgggttcgtg gattggcgcg atgtcgttca attggttcag gagcgctact
      661 acgatgtcac cgaggagcag atccgcgccg tcgtcgttga cgcggaaaaa aagcgcttcg
      721 agctcgcggg cgataaagtt cgggctacgt acggccattc tttccctatc gattttgggc
      781 agcagagcgt ccaaccgccg gatagccttt attacggcac cgcccgggat ctggcccaat
      841 cgatgctacg caacggactc aagccacgcg accggcaata cgtgcacttg agtgtttccg
      901 cggaagaggc agaatccgtc gctcgccgtc acgatcccac gccggcggtc attgtcgttc
      961 aagcaggggc ggcacacgat gaaggcgtgc gcttttaccg atcgggaccg ctattcttga
     1021 cggaaaacgt cccggcgaga tttctgtcgc tcaaataaac accctggttt actgcgtcgg
     1081 ctgagccctc tgacgccctc ggcgcggctt tgtttgcgcg cctgaaatgg ttacgttaaa
     1141 gtaactccgg gtactaacgc tctttctctg caaatttcag tgcattgatc cgtggcgccg
     1201 cttcctggcc gcgccgaatg gacgacactc gtgatgagaa gccagctcag ggaagttttg
     1261 gcccgtgcgg tcgagaagac agcgaaaacg gagaagttga acgccggcga actccctccg
     1321 atccttgtcg agccaccaaa acagcgtgag tttggcgatt tgtcgagcaa cgttgccatg
     1381 gtctgggcgc gccgcgcaaa acggccgccg cgctcgatcg ccgaagctgt gctcaagaat
     1441 ttggaggatc ccgatggaat ccttgcgcgc caggagattg ccggccctgg ttttctgaac
     1501 ttcagctttg caccgaaatt tttttaccgc gaatttcgcg agctcgtgtc aggacgaaat
     1561 cttcaaatcg atctcggcca tggagaaaaa attcaagttg agtttgccag cgtcaatcca
     1621 accggcccgc ttcacgtcgg ccacggccgt gtggcggtca ttggagacgc gctggcgcgt
     1681 ttacaccaag cggccgggtt tagcgtcgag cgcgagtatt acgtaaatga tgccggcaat
     1741 cagatggaca atctgggtcg ctcaatcagt gctcgttatc gcgaatcgtt tggcgaagac
     1801 gttgcttttc ccgaaaacgg ctacccgggc gattatgtca aagacatcgc ggctgagatt
     1861 cgaggtcaac aaggcgacaa gtatttgcat gacggagagg cggcgcagaa gttttttaga
     1921 gagacgggcg gcgatctgtt attgaacatg atccggcgac agcttaccga ttttggtatt
     1981 accttcgact ccttcttcag tgaaaaagcg atgcgcgagc gcggcgaagt cgcggaatgc
     2041 ctcgagcttt tgcgccagca ccgtcttttg tattttcagg acggcgctga atggtataaa
     2101 tcgactcaat ttggcgatga gaaggaccga acggtcatca aaagtgacgg tgagctcacc
     2161 tatttctcca gcgatatcgc atatcatcga aataaatacc agcgtggctt tcaaaaactg
     2221 atcaacgtgt ggggagccga ccatcacggc tacgtaccgc gtttgaaggc ggcgatgcag
     2281 ggactcggtt acgatccaaa tcgtttgcac gtcgtgctgg tgcaaatggt gcaactcacc
     2341 cgtggcggcg agccggtccg tatggggaag cgcagcggcg agtttatttc tttggaagaa
     2401 gttttggatg aagttggcac ggacgcggcg cgatttttct tcttgatgcg taagtcggac
     2461 agccatctcg acttcgactt ggatttagcg aagcgccaat ccagcgataa tccggttttt
     2521 tatgtccagt atgctcacgc gcgggtcgcg agcatctttg agcaggcggc gcgcaacggg
     2581 atcattttgg accagcgcga caacgtgccg atcgagcggt tggcgctcgc cgaagagcta
     2641 ggacttattc gtagaatgat tgaattcaat gacgtcctgg aagagagcgt acgggaactc
     2701 gaaccccatc ggatgatttt ttatctcgtg gatctggcca gtgactttca tcactactac
     2761 aaccacgccc gggtgatcac ggacgatgcc gatttgagcc gcgcgcgtct attattagtt
     2821 gaaaacgtgc aaaagactgt tcggcgaggg ctggaaattc tcggtattga agccccgaca
     2881 aaaatggccg ctcgggtaga aggtgaccag taaccagctc tggggccgag caatcaacga
     2941 ggcaagttca tggcagaaaa ccgcagaggt aaggaaaatc gtttctattt cagccgcgga
     3001 caaatggtct tgctcggagc ggcatttaca gccgcatctg tgatcatttt ttttcttgga
     3061 atgtttgttg gcaagggcat agaagaacgc cggctggtca a
//
LOCUS       DASULZ010000724         2270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_995857, whole genome shotgun sequence.
ACCESSION   DASULZ010000724 DASULZ010000000
VERSION     DASULZ010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995857"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VFY96_14940"
     CDS             complement(<1..561)
                     /locus_tag="VFY96_14940"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX5607819.1"
                     /translation="MDQGKNPNGRALRKNIKTIEDELRTAAKILDWEIGDLWGHVGAR
                     LPSDRGIAVKMFRRPEQGNRDWLVHFDFSLKKLSGVGTIPREAVIYTEIFKARPDVNA
                     AVHCHAAMCVALSLADKAINWIHMQSSRFRGGTPIFPRPIYILDNEEGAALAKSLGDA
                     SAVMIKGHGIVTVGESIDEACINALYM"
     gene            complement(601..1884)
                     /locus_tag="VFY96_14945"
     CDS             complement(601..1884)
                     /locus_tag="VFY96_14945"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5607820.1"
                     /translation="MLSREDNDLITRTRLGTPMGNLIRRYWMPALLSEEIPAPDCPPV
                     RVKILSEELVAFRDTNGRIGLIGEHCAHRGTSLFFGRNEACGLRCIYHGWKYDVEGHV
                     VETPAEPAGSDFRAKLRHTAYPTREANGVVYAYLGPPEKMPLFSNYEWTQVPLEHTYV
                     TKCLLECNYLQGLEGECDSSHLSFLHRAFTNERNQPLYKSDTAPFYETEETDFGVRLI
                     ATRKNNDQQYIRFSAFVMPVYGCVPAGRPTNELEGYEIHTYVPADDTHCWRYDIGFRR
                     DRTVREEEVHRRKQINPDYTRIRNLRNNYLQDRAMQKSVNFTGIEDFLNHDACATESM
                     GAIFDRSQEHLGVSDKAVIAVRRFLLNSVKELQSGKAPPHLVEDAKRNWFPHVDCFAC
                     LLPPDVSWRQQFDYLTASAQKENPMSYSLRRKVAS"
     gene            complement(1945..>2270)
                     /locus_tag="VFY96_14950"
     CDS             complement(1945..>2270)
                     /locus_tag="VFY96_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607821.1"
                     /translation="YTPVHMIVMSRKLEDHHPELAGKFYAAFEQAKQLAYDDVLSDRG
                     GFSVVYLRERMKEQIASWGDPWKYGIKANKPTIDTFIRYNLNQGMIRSAPSYDQIFAA
                     STLDT"
BASE COUNT          480 a          607 c          639 g          544 t
ORIGIN      
        1 catgtagagc gcgttaatgc aagcttcatc gatgctttct cccaccgtaa cgatgccatg
       61 ccctttgatc atgaccgcgg aagcgtcacc caaactcttg gcgagggccg cgccttcttc
      121 gttgtccaga atgtaaatcg gcctgggaaa gatcggcgtc ccgccacgga atcgtgacga
      181 ctgcatatgg atccagttaa ttgccttgtc cgccaggcta agcgcgacgc acatcgccgc
      241 atgacaatga accgccgcgt tgacatcagg ccgggctttg aagatttccg tatagatcac
      301 cgcttcgcgt ggaatcgtgc cgacgccgga aagcttcttt aaggaaaaat caaagtgcac
      361 gagccagtct ctgttgccct gctcggggcg gcggaacatc ttgaccgcga tgcctcgatc
      421 gctcgggagg cgcgccccga cgtgacccca gagatcgccg atttcccaat cgagaatctt
      481 tgcggcggtc ctcaattcgt cctcgatagt tttgatattc ttgcgcagcg cgcggccatt
      541 cggattttta ccctggtcca tatttgctcg taattttgtc tggattgatg gaagacttat
      601 ctaggaagcc actttgcgcc tcagggagta cgacatggga ttctcttttt gtgcggacgc
      661 tgtgagatag tcgaattgct ggcgccagga gacatcgggc ggcaataaac aggcgaagca
      721 atcgacgtga ggaaaccaat tgcgcttggc atcttctacc agatggggcg gtgctttgcc
      781 gctctgtagc tctttgacgc tgttcaataa aaaccggcgc acggcgatga ccgctttgtc
      841 gctgacgccc aggtgctctt ggctgcggtc aaaaatcgct cccatgcttt cggtggcaca
      901 cgcgtcatgg ttcaggaaat cctcaatccc ggtgaaattg acgctctttt gcatggcgcg
      961 atcctggaga taattgttcc gtaagttacg aatccgagta tagtcgggat taatctgctt
     1021 gcgccggtga acttcctctt cacgcactgt gcggtcgcga cgaaacccaa tatcataacg
     1081 ccagcagtgg gtatcgtcgg cgggtacata agtatgaatt tcataacctt caagctcgtt
     1141 ggtcggccgc ccggccggga cgcagccgta aaccggcatc acgaacgcgg aaaaacggat
     1201 gtactgttgg tcattgtttt tgcgtgtcgc gatcagccgg acgccgaagt ccgtttcctc
     1261 ggtctcataa aacggcgccg tgtcgctctt atagagcggc tgattacgct cgttggtgaa
     1321 tgcgcgatgt aaaaaggaga gatgcgatga gtcgcattcg ccttccaggc cctgcaaata
     1381 attgcactcg agcaggcatt tggtgacgta ggtgtgctcg agcggtactt gcgtccattc
     1441 gtaatttgaa aacaacggca tcttttcggg cgggccgaga taagcataaa cgacgccgtt
     1501 tgcctcgcga gttggataag cggtgtgccg gagcttcgcg cgaaagtcgc tgccggcggg
     1561 ctcggctggc gtctcaacga cgtggccttc gacatcgtat ttccagccat gatagatgca
     1621 gcgcaggccg catgcctcgt tgcggccgaa gaaaagcgac gtgccgcgat gcgcgcagtg
     1681 ctcgccgatc aagccgattc ggccgttggt atctctaaac gccactaatt cctcgctgag
     1741 aattttgacc cgtaccggcg gacaatccgg agcgggaatt tcttccgaca acagcgctgg
     1801 catccagtag cgccggatga gattgcccat cggcgttccc aagcgggttc gagtgatcaa
     1861 gtcgttatct tccctggaaa gcatctagac tccttatcga attgctgaaa ggttcaaaac
     1921 gttcaagccg ttcaatcctc aatgtcacgt atcgagcgtg ctggcggcga agatctggtc
     1981 gtaggacggc gcgctccgga tcatgccttg attgaggttg tatcggataa aggtgtcgat
     2041 ggtcggcttg ttggctttga taccatattt ccatggatcg ccccatgagg cgatttgttc
     2101 tttcatgcgc tccctgagat agacgactga gaagccgccg cggtcgctga ggacgtcatc
     2161 ataggcgagt tgctttgcct gttcgaaagc ggcgtagaac ttaccggcga gctcaggatg
     2221 atggtcctcc agtttccggc tcatcacgat catatgcacg ggcgtataaa
//
LOCUS       DASULZ010000725         2905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_488828, whole genome shotgun sequence.
ACCESSION   DASULZ010000725 DASULZ010000000
VERSION     DASULZ010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488828"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VFY96_14955"
     CDS             complement(<1..403)
                     /locus_tag="VFY96_14955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5607822.1"
                     /translation="MSLPGQYFQKLVESCPDIIISVDNHGTIIFYNDGARQNLGFSDE
                     EVLGKNVAEIYPSLAEARRVMTAMRACRGRVSNFETVFKTRKGEQIPVAISASITYDD
                     NGSEIGSIGFAKDLREIRRRDQLVTMGEIAIG"
     gene            509..2347
                     /locus_tag="VFY96_14960"
     CDS             509..2347
                     /locus_tag="VFY96_14960"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX5607823.1"
                     /translation="MAPTHDTRQLNWLLAWAVVFCDIGTSVYYVPGILYGHVRDATPF
                     FVLLTTGGFILLSLKYIEISWRNPEGGGVVTITTKAFGPMWGCLGGMLITVDYFLTTA
                     ISAVSGFHYIGSAFAGLDSPVVLLSCTGVLVLAALNIVGIRESAAVALVMACASFIVN
                     LLVIGTAMLNMNPTEWHTVLSRLGTGPELSFYDLLVGFASAWLAFSGLESISQLSPAM
                     RFPLRKTTRRAMLAVIVTMVVTAPILTALSIGLLPQQIKATESERFISELGMSVGGLG
                     LKLAVILTASSLLLFASNTAIIGSYHVFLALVKGGFLPKIIAARNGTFNTPHIAIAIA
                     TLVPVLVILLSHGEMKVLGDMYAFGLLGAFVFSSLSLDSIRWRLGRRDPGFWIGVFTT
                     AMVVVAWAVNLVEKQLATYFGGAVTAIGMITAIGVRRAWFLDLAVRVPFIQRLQAQAY
                     RASEDLVEDELKGLVTLSEAVEVKPLYSSSTLLAIRDESPRLIQEGLTRAKGRGESAI
                     YCIYVEEWPGLFAGETPHAPNEEGVRTLRAALQAGRQNNTEVIPIWSVSHNAAQAIAN
                     AAKALGVDAVMIGASRRSAFYHMLRGRVVRGLMKKLPRDCHLMISN"
     gene            complement(2464..>2905)
                     /locus_tag="VFY96_14965"
     CDS             complement(2464..>2905)
                     /locus_tag="VFY96_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607824.1"
                     /translation="PNTLKAKESGLNMLVDIGKLNIPAFQVAYGTTEKYLKNNPNTVY
                     AFLKAIAEGVVLSRKDPGVAKKAIGKYAKIEDAPTIDGTYDAFAPYWAMNLAVRPEAI
                     KAQFEYLDEKESANAKNADPKDFYDNSFVETLSRSGFLKGLGMR"
BASE COUNT          559 a          804 c          786 g          756 t
ORIGIN      
        1 ggccgatagc gatttcgccc atggtcacca gctgatcacg ccgtcgaatc tcgcgaaggt
       61 ctttggcgaa accgatggag ccaatttcac tgccgttatc gtcgtaagtg atcgatgccg
      121 agatcgccac cgggatctgc tcgccctttc tggttttgaa gacggtttcg aaattgctca
      181 cccgccctcg acaagcgcgc atggctgtca tcactctgcg tgcctcggcg agactaggat
      241 aaatttcggc gacgtttttg cccaacactt cttcgtcgct gaagccgaga ttttggcgcg
      301 cgccgtcgtt ataaaaaatg attgtgccgt ggttatcgac ggaaatgata atgtcagggc
      361 aactctcgac gagtttttgg aagtattgtc cgggcagcga catacgcgca ggtatcttgt
      421 atgatacggt gaacaacgat atcaatgcaa gagcgaaacg gcaatcgctc gctcgtcgcg
      481 cctagtgctc gttccagcag ctcagaatgt ggcaccgact cacgacaccc gacaattaaa
      541 ttggcttttg gcctgggccg tcgtcttctg cgacatcggc acttcggtct actacgttcc
      601 cggaatcctc tacggccatg tccgggacgc gacgcctttc tttgttttgc tcaccaccgg
      661 cggcttcatt cttctgtcgc tcaagtacat tgaaatttcc tggcgtaatc ccgaaggcgg
      721 cggcgtcgtc accatcacga ccaaggcgtt tggtccaatg tggggatgtc ttggcggaat
      781 gctgattacc gtggattatt ttctcaccac cgcgatctca gccgtttcgg gatttcacta
      841 catcggcagc gcttttgccg gactggacag ccccgttgtt ctgctttcct gcaccggtgt
      901 gctggtgctc gccgcgctca atattgttgg cattcgcgag agtgcggcgg tcgctctcgt
      961 catggcatgc gcttcattta tcgtaaacct gctcgtgatc ggaacggcga tgctgaacat
     1021 gaatccaacc gagtggcata cggttcttag ccggcttgga accggaccgg agctttcctt
     1081 ttacgatcta ttggtcggct tcgcgtcggc ttggctcgct ttttccggat tggaaagcat
     1141 cagccagctg agtccggcaa tgcggtttcc gctccgcaag accacccggc gagcgatgct
     1201 cgcggtgatc gtcaccatgg tcgttaccgc gccgatattg accgcgctct caatcggcct
     1261 gttgccccaa cagatcaaag ccactgaaag cgagcgcttt atctcggagc tcggcatgtc
     1321 cgtcggcggt ttaggtctaa aacttgccgt gatactcact gcctcgagcc tgcttttatt
     1381 tgcgtccaac accgcaatta ttggctccta tcatgttttt ctcgcactgg taaaaggcgg
     1441 atttctgcca aaaatcatcg ccgcgcgtaa cggcaccttc aatacgccgc atatcgcgat
     1501 tgcgatcgcg acgctcgttc ctgtcctggt cattcttctg agccatggtg aaatgaaggt
     1561 gctgggggac atgtatgcct tcggtctgct cggcgcgttt gttttttctt ctttgagctt
     1621 ggattcaatt cgctggcgcc tgggccggcg cgatcccggc ttttggatcg gcgtctttac
     1681 gactgcaatg gtcgtcgtcg cctgggcagt taatctggtc gaaaaacagc tggccacata
     1741 tttcggcggc gcggtaactg cgatcggtat gatcaccgcg attggtgtgc gccgcgcttg
     1801 gtttctcgat ctcgctgttc gagtgccgtt catccagcgt cttcaggcac aggcgtatcg
     1861 cgcgtccgag gatttggtcg aggatgagct caaggggttg gtgactcttt ccgaggcggt
     1921 ggaggtgaag ccactttatt catcgtccac gctgctggcg atacgcgacg aatcgccgcg
     1981 actgattcag gagggtctca cgcgtgccaa agggcgcggg gaatcggcga tctactgtat
     2041 ctatgtagag gaatggcccg gtttattcgc tggcgagacc cctcatgctc ccaacgagga
     2101 aggggttcgc acattgcgtg cggcgctcca agccggacgg caaaacaaca cagaggtgat
     2161 ccccatctgg tcggtttcgc acaacgccgc tcaggcgatc gccaatgcag ccaaggcgct
     2221 gggtgtcgat gcggtgatga tcggagcttc gcgccgctcg gccttttatc acatgctgcg
     2281 cggccgcgtc gtccgcggct tgatgaagaa gctgccgcgt gactgtcatt tgatgatttc
     2341 caattaacgg cggagcaact tatcaactaa ggtattcatt ctcgctcgct ctctgtaagc
     2401 gatcgatgcg cttcttgcac gcctaagagc catcgagtgt cacaaacggc cgcctcagga
     2461 catctaccgc atgcccaaac ctttcaggaa tccagacctg gataaagttt cgacaaaaga
     2521 gttgtcgtaa aaatccttcg gatctgcgtt cttcgcattg gcggattctt tttcgtccag
     2581 gtattcgaat tgcgctttga tcgcttccgg acgcactgcc aaattcattg cccagtacgg
     2641 cgcaaaggca tcatacgttc catcgattgt cggagcatct tcgattttgg cgtatttccc
     2701 aatggctttt ttggcaacac caggatcctt gcgtgacaga acaacacctt cggcaatcgc
     2761 tttgagaaat gcgtagaccg tgttgggatt gttcttgaga tatttttctg tcgtgccgta
     2821 tgcgacctgg aacgcaggaa tgttgagctt cccgatatca accagcatgt tcagccccga
     2881 ttcttttgct ttcaatgtat tcggc
//
LOCUS       DASULZ010000726         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1096447, whole genome shotgun sequence.
ACCESSION   DASULZ010000726 DASULZ010000000
VERSION     DASULZ010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096447"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..1303
                     /locus_tag="VFY96_14970"
     CDS             230..1303
                     /locus_tag="VFY96_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607825.1"
                     /translation="MKSKQFLSFFRSTFFVVMALSLLPGSAASQSSFFQGKTLTIIQG
                     RDPGGTGDLRVRALFPFLHKYIPGSPTIVSEYMPGGGSRKAANHIFRNSRPDGLTLGN
                     FSSAMVSLAVLGESGVLYDIDKFHYLGSPYSTYHAVFVSRKEAGFNTIEKLRAASGVK
                     IGAQSLGFSTYTEGRLFAYMIGLKDPLFIAAFGGAELDPALMRGEIDARATGPDTILA
                     RNRDWIDKGLADIHAIMETPKGDKHPHPFFAKLPEIESFARNDKERKIIALQRAFRIA
                     GTPFVLPPGTPKDRVEVLQEAFRKTYRDPEFAKEYKKLTADDPTPLLPENHEKAIREI
                     PRDPEVIELFKVLVGPKKLPPRA"
     gene            1517..>2168
                     /locus_tag="VFY96_14975"
     CDS             1517..>2168
                     /locus_tag="VFY96_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607826.1"
                     /translation="MTTLHVLSVRLSAHALLIGSLFSLFPPAFGQTPFYQGKTITVVV
                     GTSPAGTGDLRVKALIPFLRKHIPGNPALVLEYMDGGGGRKAANYMYRTARPDGLAIG
                     AMSSGVISLNIMRESGVLYDLDKFIYLGAPETAGHQTIYTRKDLGLDSLEKLRSKSGL
                     RIGAQTVGHVSYIAGRFFAYFLDFKEPKFIAGYTAPEVDAALLRGELDARANAAVSV"
BASE COUNT          503 a          603 c          568 g          494 t
ORIGIN      
        1 aaaacgatgc aggatgaaca attccgcgct gaagtaaaaa agcgcaatta tgaattcgat
       61 ccagtcggcg gcgaagaact gggaaagcta gccaaggatg tgaccgcaca accgccggaa
      121 gtcatggatc gattgaaaaa gctattggcc aattgattct agtgggctgg aataccgccg
      181 cggtatgccg tgttcttaca accgaacgaa gtctcatgag ggagtcctta tgaaaagcaa
      241 gcagttctta tcgtttttca ggtcgacgtt ctttgtcgta atggctttgt cgctcttgcc
      301 gggatccgct gcttcccaaa gttcattctt tcaggggaag accctcacca ttattcaagg
      361 gcgagatccc ggtggtacag gagatttaag agtacgagcg ctctttccat tcctccacaa
      421 gtatattccc ggcagcccga cgatcgtgag cgagtacatg cctgggggcg gcagtcgaaa
      481 ggcggccaat cacattttca gaaattcccg ccctgacgga ttgaccctcg gcaactttag
      541 ctccgccatg gtttcgcttg cggtccttgg cgaatcgggc gttctatacg acatagacaa
      601 attccactac ttggggtctc cctacagcac ctatcacgca gtttttgtca gccgcaaaga
      661 agccggtttc aacaccatcg aaaaattgcg cgcggctagc ggcgtcaaaa tcggcgctca
      721 gtcgctcgga ttctcgacct atacagaagg gcggttgttc gcctacatga ttggactcaa
      781 ggatccattg ttcatcgctg ctttcggcgg cgccgagctc gatccggcgc tcatgcgtgg
      841 cgagatcgat gcccgcgcga ccggacccga tacgattttg gcgcgtaatc gcgactggat
      901 cgacaagggt ttggccgaca ttcacgcgat catggaaacg ccgaaaggcg acaaacatcc
      961 gcatcccttc ttcgccaagc ttcccgagat cgaaagcttt gccagaaacg ataaagagcg
     1021 caaaattatc gctctgcaac gcgccttccg tattgccgga acaccgtttg ttcttccgcc
     1081 tggcacgccc aaggatcggg tcgaggttct tcaggaagct tttcgcaaga cttatcgcga
     1141 tcccgaattc gccaaggagt ataaaaaact caccgccgac gatccaacgc cgctgctgcc
     1201 ggaaaatcac gagaaagcga tcagagaaat tccccgcgac ccggaagtga tcgagctatt
     1261 caaagtactg gtgggaccaa aaaaattacc gccgcgagca tagcttaagc catcctcctt
     1321 agcatcgaga ctgtgtcgga atgtacaagt ggcctctatt gtcatcccga gcgagagatc
     1381 ttgcattctc aattgcgtac gaagaaagat ttctcgcctt tggctcgaaa tgacacattc
     1441 cgacatggag ctggaggaat gaactgagcg ccgacagttc agtctgatga tgtgaacttc
     1501 tccatcggga ggactcgtga ccacactgca tgttttatct gttcggttat cagctcacgc
     1561 gctgctgatc ggttcgctat tttcgttatt cccacccgca ttcgggcaga cccccttcta
     1621 ccagggtaag acgatcactg tggtcgtcgg cacctcgccc gccggaaccg gcgatctgcg
     1681 cgtgaaagcg ctgatacctt ttttgcggaa gcacattccg ggaaatcccg cgctggtgct
     1741 cgaatacatg gacggcggcg gcgggcgcaa agcggccaac tacatgtacc gcacggcgcg
     1801 gcctgacggc cttgcgatcg gtgcgatgag cagcggcgtt atttcgctca acatcatgcg
     1861 cgaaagtggc gtactctacg atctcgataa atttatttac ctcggcgcgc cggaaaccgc
     1921 cggccatcaa acgatctaca cgcgcaaaga tcttggtttg gacagtctcg aaaaacttcg
     1981 ctcgaagtcc ggccttcgta tcggcgctca gactgtgggt cacgtctcgt atatcgcggg
     2041 gcgattcttc gcttactttc tcgatttcaa agaaccgaag tttatcgcag gttacaccgc
     2101 tcccgaagtc gacgctgccc ttttgcgcgg tgagctcgat gcgcgcgcga atgccgccgt
     2161 gtcggtat
//
LOCUS       DASULZ010000727         3582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_996021, whole genome shotgun sequence.
ACCESSION   DASULZ010000727 DASULZ010000000
VERSION     DASULZ010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996021"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VFY96_14980"
     CDS             complement(<1..454)
                     /locus_tag="VFY96_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607827.1"
                     /translation="MSDQRLTQLMAAIVATAVAAPLFAQQAPNPNSPSSNVPTPTAPA
                     QPPTTQQAPTVVPRRIVPPDRAPAQVQGQQAAPQAPQAAPASPEAAPGPDTTPTPSAP
                     AQRRFSAAPAPGGMISLNFNRADLVEIIHIIAQQLRLTYTIDPEVKGTV"
     gene            complement(478..1089)
                     /locus_tag="VFY96_14985"
     CDS             complement(478..1089)
                     /locus_tag="VFY96_14985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607828.1"
                     /translation="MALATGFVCWSTYRTWNDDPWILPAPAKAISLPVSEKPASVSAK
                     PSINTDVIVSKNLFDPERGAGATREAEENSRSSQRVRNLILLGTVITDNNRTAVVQDA
                     GPAPAQGAAAGQPAAPIRLKLGDDFEGYRLTEIADRRIVFTKDRARVDVVLDYFRKVE
                     VSPPRAVPPAQIVPPGQAAPPPRVLPNLPRRGRVPVPGNPAPE"
     gene            complement(1278..1943)
                     /gene="gspM"
                     /locus_tag="VFY96_14990"
     CDS             complement(1278..1943)
                     /gene="gspM"
                     /locus_tag="VFY96_14990"
                     /inference="COORDINATES: protein motif:HMM:NF040576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HEX5607829.1"
                     /translation="MKAIWQKFSKRERGLVVLTALVLLAFVAKYAVITPFLQRREWVK
                     RQLESQPQLLTKNLRYVGQKEAMLAALDAARVETKAQEPKLLTGDTPSVSASDLQETV
                     QALASREGTQVITTRVLNPEPAGSFSKIGIQMEIGGQIQQVANLIKGIETSPKLLVID
                     EINVRSLFRPIGFPQSPGVPQLPAQNLRVSITIVGFARAQPALGGKPESPANPTKSSS
                     RGV"
     gene            complement(1940..3502)
                     /locus_tag="VFY96_14995"
     CDS             complement(1940..3502)
                     /locus_tag="VFY96_14995"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEX5607830.1"
                     /translation="MTALRNLQQISRADFLTGVGLYFLSDRLVMARLRKNLLQVSVLE
                     QEVRELPESDNRQAISELTGWVAEDVREIALKAASDSRERALRQAVVSLLPHLNPTRD
                     AVYLCLPPENAVVQEVLLPLAAQENIAQVLEYELERQLPFKRDDVYYDFIPVGKKGER
                     FSVYLFALPKKNVDTLLSLLDSLDIKVAGVETTITALANYLLFSDGSNAGAVGIVTAR
                     PRYWEMIAFKTRSDSWQPDTQLLFCNQMSGADWAQATGKELLQENMSLIPKVFRCGDL
                     SALNGLAHEHLAGAEDLAELGSVRLKGMTAPLQAETLPAIGAALRGVREASFSANLLR
                     HEGSDRDRNKGLSLLNVLLLGFFVIALAAWGVSYPIKDELRLRQLQNENRKLEPAVSA
                     LQREENQLEQLRKQAGLLTELERRRGETLRVLDELSKIVPNTVYFSNLRYRAGSLELQ
                     GNAENASTLIPLLERSPVFENVGFNAPSNRGRDNRETFSLKADLEKPMPSTGSTTKAP
                     ASAPPKETKAAR"
BASE COUNT          768 a         1013 c          999 g          802 t
ORIGIN      
        1 ttactgtccc tttcacttcc ggatcgatgg tgtaggtgag ccgcaattgc tgcgcgatga
       61 tgtgaatgat ttcgaccaag tcggcgcggt tgaagttcaa cgagatcatg ccacctggcg
      121 ccggtgctgc agaaaatctt cgttgtgccg gcgcagaagg tgtgggcgtg gtgtcgggcc
      181 caggagcggc ttccggcgaa gctggcgctg cttgtggagc ttggggcgca gcctgctggc
      241 cttggacctg agccggggct cgatccggcg gaacgattcg acgcggtaca accgttggcg
      301 cctgctgcgt tgtcggaggt tgagccggag ccgtcggggt cggaacgttc gaactgggcg
      361 aattcggatt tggcgcttgc tgggcgaaca gcggagcagc gacggcggtc gcaacgatcg
      421 ccgccatcaa ttgggtcagg cgctggtctg acatcaaggt aattcctcct aaccttttca
      481 ctccggcgcc ggatttcccg gcactggtac tcgaccgcga cgcggcaggt ttggcagaac
      541 gcgcggcggt ggggcagctt gccctggcgg aacgatttga gcgggcggca ccgcgcgcgg
      601 cgggctaacc tcgactttac ggaaatagtc gaggacaacg tctactcttg ctcggtcttt
      661 ggtaaagaca attcgtctat cagcaatttc agtgagcctg taaccttcaa aatcatcacc
      721 gagcttcagc cggatcggcg ccgcgggttg gccagccgcc gccccttgag ccggtgcggg
      781 tcctgcgtcc tgaaccacgg cagtgcggtt gttatcggtg ataacggtgc ccaaaagaat
      841 cagattacga acgcgctgag atgaccgcga attctcttcg gcttcacgag ttgcgccggc
      901 gccgcgctcg ggatcaaaca agtttttgct gacgatcacg tccgtattga tcgacggttt
      961 ggcgcttacc gaagcgggct tctcagaaac cggtaatgaa atcgcttttg caggtgccgg
     1021 gagaatccat ggatcatcgt tccacgtccg gtatgtgctc caacaaacga atcccgtggc
     1081 gagtgccaac aaaatactca gcagtgaaga tcgggttaga atcgctcttg ctccatcgaa
     1141 taggtcgaga aatgccctaa tttacgccca ccgcgaacaa gcgtcaattc aaaattcgct
     1201 tgcaggtcgg tttaggaact tgctccgcca ggcaatgctt gaaaaacttc ggccgcagcc
     1261 ggcccgcctc cggcgtatta aacacctctc gacgaagatt tggtcggatt agcgggcgat
     1321 tcgggttttc cacccagtgc cggctgtgca cgcgcaaaac ccacaatcgt aatacttacg
     1381 cgcaaattct gcgcgggtag ctgcggcacg cctggcgact gaggaaaccc aatcggacgg
     1441 aagagcgaac gcacattaat ctcatcgatc accagcaatt tgggggaggt ctcaatacct
     1501 ttaataagat tggcgacttg ctgaatttgg ccgccgatct ccatttggat gccgattttt
     1561 gaaaatgatc ctgccggctc gggattgagc acccgcgtgg taatgacctg cgtaccctcg
     1621 cggctggcta atgcttgcac ggtctcctgc aagtccgaag cgctcaccga gggtgtatcg
     1681 ccggtaagaa gttttggttc ctgcgcctta gtctccacgc gcgcggcatc gagggccgcg
     1741 agcatggctt ctttttggcc aacgtaacga agatttttcg tcaataattg tggttggctt
     1801 tcgagctgtc gcttgaccca ttcgcgccgc tgcagaaacg gtgtgatcac cgcatatttt
     1861 gcaacaaatg ccaggagaac cagcgcggtc aataccacca aaccgcgctc gcgcttggaa
     1921 aatttttgcc aaatcgcttt catcgggctg ctttggtttc cttcggcgga gcactggcgg
     1981 gtgccttagt tgtgctgcca gtactcggca taggcttttc caaatcggcc ttcaacgaga
     2041 aggtctcacg gttatcgcgg ccgcgattcg acggcgcgtt gaagccaacg ttctcaaaca
     2101 ccggcgagcg ctccaggagt ggaattaacg ttgaagcatt ttccgcattc ccttgcagct
     2161 ccaacgaacc ggcccggtat cgcagatttg aaaagtaaac cgtgttcggg acgattttgg
     2221 agagctcgtc gagcactcga agagtttccc cccgccgccg ctcgagctcc gtaagcagcc
     2281 cggcttgctt tcgcagctgt tcgagctggt tttcctcgcg ctggagcgcg ctgacggctg
     2341 gctcgagctt gcgattttcg ttttgcaact ggcgtaagcg aagctcatcc ttgatcggat
     2401 agctcactcc ccaggctgcc aacgcaatga cgaagaaacc cagcagcaag acattgagca
     2461 acgacaagcc cttgttgcga tcacgatcgc tgccttcatg acgcaacaaa tttgcgctga
     2521 aggaggcttc ccggaccccg cgcaaagctg caccgatggc cggcaacgtc tcggcttgaa
     2581 gcggcgccgt catgcccttc aggcgaacgc tgccgagctc ggccagatct tcggcgcccg
     2641 ccagatgctc atgcgccaat ccattcaaag cggataaatc tccgcagcga aagaccttgg
     2701 gaatcaggct catattctct tgcagcagtt ctttaccggt agcttgagcc cagtcggcgc
     2761 cggacatttg attgcagaag agcaattgcg tgtccggctg ccagctatcc gagcgcgtct
     2821 taaaagcaat catttcccaa taccgcgggc gcgccgtcac gatgccaacc gcgccggcat
     2881 tcgatccatc actgaaaagc agataattgg ccagagctgt aatcgtcgtc tcaacgcccg
     2941 cgacttttat atctaacgaa tcgagcaaac tcagtagcgt atcgacattt ttttttggca
     3001 gcgcaaacaa atagacgctg aacctttcgc ctttctttcc taccggaata aagtcgtagt
     3061 aaacatcatc gcgtttgaat ggaagttgcc gctcgagttc atattcaagg acctgcgcga
     3121 tgttttcttg cgcggcaagt ggtaaaagaa cttcttgcac caccgcgttt tcaggcggca
     3181 ggcaaagata gaccgcgtcg cgcgtaggat tgaggtgcgg caaaagtgaa accaccgcct
     3241 ggcgtagtgc ccgctcccgt gagtcacttg ccgccttgag cgcgatttcc ctcacatctt
     3301 cagcgaccca acccgtcaat tccgaaatcg cctgacgatt atcggattca ggaagttccc
     3361 ggacctcctg ctccaggacc gacacttgca gcagattttt tcgcaaacgc gccatcacca
     3421 ggcggtcgct gaggaaatac aatccgacac cggttagaaa atccgcgcgc gaaatctgct
     3481 gcaggttacg aagtgcggtc atcgtcggca cttaaaaacc atttgaggcc attgaacgat
     3541 tggtcgtcat attgtaaccc aagcgcaaat tttgtggtag tg
//
LOCUS       DASULZ010000728         2218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1199713, whole genome shotgun sequence.
ACCESSION   DASULZ010000728 DASULZ010000000
VERSION     DASULZ010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199713"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            325..759
                     /locus_tag="VFY96_15000"
     CDS             325..759
                     /locus_tag="VFY96_15000"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX5607831.1"
                     /translation="MVAHASNSHANGKSPAHSAFQMYFGSLEAIGQAYDPFLKGLART
                     QLEMLGFFNRRAQSYMQIPARAAQCRTPQDLMTVQAQFWRSAYEDYTESVGRVTNALA
                     AVGLPNFAAISEEALHNAHDYIAFPETKDQAGGNRARERKAA"
     gene            complement(849..1331)
                     /locus_tag="VFY96_15005"
     CDS             complement(849..1331)
                     /locus_tag="VFY96_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HEX5607832.1"
                     /translation="MAALFAALVATTLPGMAQEGTVKAQHGDWQIVCKDPPPGAKNEV
                     CALVQSVTAEDKDNIGLTVYFQKFSNGTRVLRVFAPLGILLPPGLGLKIDDKDVGHAP
                     FLRCHTFACYAQVVVEDPLIEQLKNGKTAIFIIFQAEEAGIGIPISLGGFKEALAELK
                     "
     gene            1783..>2218
                     /locus_tag="VFY96_15010"
     CDS             1783..>2218
                     /locus_tag="VFY96_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX5607833.1"
                     /translation="MLLLGTSVAWAAMGQPSPGEMGFQGAASPVAEEIHRFYDFVNII
                     IVAITVFVMLLLLYVMYRFNERANPAPSKVTHHTMLEVAWTVVPILILVAIAIPSFRL
                     LNLQYSYPKADLTLKATGYQWYWEHAYPELGIAFETRMLDEAG"
BASE COUNT          427 a          685 c          664 g          442 t
ORIGIN      
        1 gccagcgctg tacgcttttg agagacgccc ccgccatgga aatacgcaga actgcagaat
       61 gccgaccacg aaataaacct gtggattaac caagaaagag ggatgtgcaa cctcaagtca
      121 atgcaattct cgctcgcggc atggataacg attgcgcgac gtcaggtgat tcgacctgtg
      181 catagaacca atttctgtca tcctgctgac cacttcagtt gataagattt ctttcgtagg
      241 gttgcttcaa accttcaatg gagacggcct ccagccccgg ctcccccgcc gcaatcaggc
      301 cgtccgcaat agggaggacg taccatggtt gctcatgcgt cgaattcgca tgcgaacggc
      361 aaatcccctg ctcacagcgc ctttcagatg tacttcggca gtctcgaggc aatcggccaa
      421 gcctacgacc cgttcctgaa gggcctcgct cgcacgcagc tcgagatgct cggcttcttc
      481 aatcggcgtg cccagtccta catgcagatc ccagcccgcg ccgcccagtg ccgtaccccg
      541 caagacctga tgaccgtgca ggctcaattc tggcggagcg catatgagga ctacaccgag
      601 agcgtcggcc gcgtgaccaa tgcgctcgcg gcggtcggtc ttccgaactt cgcggcgatt
      661 tcggaggaag ccctccacaa cgcccacgac tatatcgcct tccctgagac caaagatcag
      721 gcgggcggca accgggcgcg ggagcgcaag gcggcctagg tgatccaacg agctaggagc
      781 tctcgccggc ggcgcgatcg cgccgcggca ggggagtgac tggtcttttt tctaggcctc
      841 agctcgggtc atttcagctc tgcaagagct tccttgaaac cgccgagcga gatcggaatg
      901 ccgatacccg cctcttccgc ttggaagatg atgaagattg ccgttttccc gttcttcaac
      961 tgctcgatca gcgggtcctc aaccacaact tgcgcgtagc aggcgaacgt gtggcagcgc
     1021 aaaaacggcg cgtggcccac atccttgtca tcgattttca gcccgaggcc cgggggcaga
     1081 agaattccca gcggcgcgaa tacgcgcaaa acgcgcgtgc cgttcgagaa tttttggaag
     1141 tagacggtga ggccaatgtt gtctttgtcc tcggccgtga cgctctgcac cagagcgcaa
     1201 acctcgttct tcgcgccggg cgggggatcc ttgcagacga tctgccagtc gccgtgctgc
     1261 gccttgacgg taccctcttg cgccatgccg gggagagtgg tggcgacaag agcggcaaaa
     1321 agcgctgcca gttggaagcc tccgcggcgg gcgagcgcgc ttagtgcgct gcggcaaagg
     1381 gggccggcag cagggtcgat tttgtgcacg cgctgctcct gaggtctgcg ccggcgtcgc
     1441 gtccgacgcg cgatcgtcgt gttcccggaa tcgggccaaa gtttggacct gacgtaggtc
     1501 agacttttcg ctgatgcgag cggattttta agatccgccg caacgcgtgg aaatgccgca
     1561 ttaagcttga cttgaggcat ggaagtcgat tgtgctcttt tcgcaattgg ggacgtgcgt
     1621 cctgcgctcg gcggcttcgg caccggcgcg gttgttgcgg gcgaactgga tcaaaaacag
     1681 cgcctgtcgt ggggagggtc tcggccaacg agccgagcag cacttgggag cggatgaatg
     1741 ttgagacgac tgctttgctc ggcgcctgcc gcagcatgtg tgatgctgtt gcttgggact
     1801 tccgtagcgt gggccgccat gggccagccg tcgccgggcg agatgggctt ccagggcgcc
     1861 gcctcaccgg tggccgagga aatccatagg ttctacgact tcgtcaacat catcatcgtg
     1921 gccatcaccg tcttcgtgat gctgctgctg ctctacgtga tgtaccgctt caacgagcgc
     1981 gccaacccgg caccgtcgaa ggtcacccac catacgatgc tcgaggttgc ttggacggtc
     2041 gtcccgatcc tcatcctcgt cgccatcgcc atcccctcct tccgcctcct caatctccag
     2101 tacagctatc caaaagcgga cctcacgctg aaggcgaccg gctaccagtg gtactgggag
     2161 cacgcctatc ccgagctcgg catcgcgttc gagacgcgca tgctcgacga agcaggcc
//
LOCUS       DASULZ010000729         3008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_996261, whole genome shotgun sequence.
ACCESSION   DASULZ010000729 DASULZ010000000
VERSION     DASULZ010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996261"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VFY96_15015"
     CDS             <1..741
                     /locus_tag="VFY96_15015"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF044283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX5607834.1"
                     /translation="EYDFIGGIQGEPVDVIRGEFTGLPIPATAEIVLEGECVPGPEKI
                     EGPFGEWTGYYGSSARPEPIVKVHTVMHRNDPILLGYTRKWRAPLKAALVWDDLDAAG
                     VPDVRGVWYHEAAGAAYLFLVVSIKQRYPGHARQAGLIATECHAAAYLGRYTIVVDDD
                     IDPTNLNEVLWAVCTRSDPSVDIDILRRCWSGPLDPIIPKDHKGFNSRAIIDATRPYE
                     WKDTFPKVSRSSQELRDKILAKWADRLS"
     gene            836..1798
                     /locus_tag="VFY96_15020"
     CDS             836..1798
                     /locus_tag="VFY96_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607835.1"
                     /translation="MALQLTIACGDYDRTHPLIDGSIKPEGLELNWLVLPHLEIWTRM
                     LNYYDFDASEISLSSYITAKAMGKPLTAVPVFPARAFRHSYIFINTKSGIREPKDLTG
                     KRIGLGEFQQTATVWMRGMLQHEYGVNLDEIEWYLWTPHARMEMELPKRYKVKRITPD
                     RKPDQMFFDGELDAIMVPSLFPSLFNPPPHIHRLFEDAPKIEAEYYKRTGIFPIMHAI
                     ALRQNVWEEHPWIARSLFKAFQRAKEDAYARLKDLSPYKISLAWFRGPVEEQERILGN
                     DPWPYGLEKNRHVVETLATYLHEQGLVDRKMPLEELFAPNTLDL"
     gene            1902..>3008
                     /locus_tag="VFY96_15025"
     CDS             1902..>3008
                     /locus_tag="VFY96_15025"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5607836.1"
                     /translation="MKNGFRCFDSDMHVYDAANLYARYMNPKWGDRIPRGERNGKHGR
                     VEFSIAGGKETLRGITDVIDHGQKQVADRYDFAVAREYDALSQVEAMDREGLDVAVLF
                     RTSPLHCDETLEPEYANDLCRAWNNWIADFCNVNPQRLKASALITLHDVELAVEEVRR
                     VVQQLGIRALSLCPEPINGRRIHDRCFDPLWAEIENLGVALCFHPPARPKQEQIANRF
                     FGHPNANIVALALRNPLELIQAVASFCAGGVLEKFPKLRVAFLEGNCAWLPWLLYRLD
                     ERAKLHGPLADVPLSRKPSDYFLEQCFISVDPDEYLVTDVIKRIGDNNLVVSTDYPHI
                     DAHFPHAIDEFLEIEGLSQSSRRKILWDNCARLYN"
BASE COUNT          687 a          792 c          854 g          675 t
ORIGIN      
        1 gaatacgact tcatcggcgg cattcaaggt gagcccgtgg acgtcatccg cggtgaattc
       61 acgggcctac cgattccggc cacggcggaa atcgtcctcg aaggggagtg tgtgccgggg
      121 ccggaaaaga tcgaaggtcc tttcggcgaa tggaccggat attacggcag ctcggctcgt
      181 ccggagccga ttgtaaaggt gcacacggtt atgcatcgaa acgatccgat cctcttgggc
      241 tatacacgta agtggcgtgc gccgctcaaa gcggcgctgg tatgggatga tttggacgcg
      301 gccggcgtgc ctgatgtgcg cggtgtgtgg taccacgaag cggccggcgc ggcatatcta
      361 tttctcgtcg tgtcgatcaa gcagcgttat cccggccatg cgcgccaggc gggattgatt
      421 gcgaccgaat gccacgcggc cgcctacctt gggcgctaca ccatagtagt cgacgatgac
      481 atcgatccga ccaacttgaa cgaagttttg tgggccgtgt gcacgcgcag tgatccgtcg
      541 gtcgacatcg acatcctgcg ccgctgctgg agcggcccgc tggatccgat cattcccaaa
      601 gaccacaaag gcttcaactc gcgcgcgatt atcgacgcaa ctcgcccgta cgagtggaaa
      661 gacacctttc ccaaagtgtc gcgctcgagc caagaattgc gtgacaaaat tcttgcgaaa
      721 tgggcggaca ggttatcgta ggttggagga cgcgaagaac acgaaggtct ggaatcaaga
      781 aagggcgcag catgctgcgc ccgtctcgtg ctcagcaaaa tagtatggag tcaccatggc
      841 actgcaactg acgatagcct gcggcgatta tgaccgtacc cacccgttga tcgatggctc
      901 gatcaaaccc gaagggctcg agctcaactg gctcgtcctg ccgcatctcg aaatctggac
      961 gcgcatgctg aactattatg atttcgatgc ttcggagatt tccttgtcgt cgtacattac
     1021 cgccaaggcg atgggtaaac cgttaactgc agtcccggtt tttccggcgc gagcctttcg
     1081 acactcctat atttttatca ataccaagag cggaattcgc gagccgaagg atcttacagg
     1141 taaaaggatt ggccttggcg agtttcagca aaccgctacg gtctggatgc gcggcatgct
     1201 gcagcacgaa tacggcgtca atctggatga aattgaatgg tacctctgga cgccacatgc
     1261 gcggatggag atggaactgc caaagcgcta caaagtaaag cggatcacgc ccgatcgtaa
     1321 gcccgatcag atgtttttcg acggcgagct cgacgccatt atggtgccga gtttgttccc
     1381 gtcgttgttc aacccgcccc cccatattca ccggttgttt gaggatgcgc cgaaaatcga
     1441 agcagaatat tacaaacgaa cgggcatctt tcccattatg catgccatag cgttgcgcca
     1501 gaacgtctgg gaagaacacc cctggatcgc acgcagtctc ttcaaggcct ttcagcgcgc
     1561 caaggaagac gcctatgcgc gattgaaaga cttatcgccg tacaaaatca gtcttgcctg
     1621 gtttcgtggg ccggttgaag aacaagagcg aattctcggt aatgatccgt ggccttacgg
     1681 tttggagaaa aatcgccacg tggtcgaaac attggcgact tacctgcacg agcagggatt
     1741 ggtggacaga aaaatgccac tggaagaatt gtttgcaccg aatacgctgg atttatgagg
     1801 gccgagttca atcggttcaa ctcgttcaat acgttcaagc ggtttcactc cttcggaacg
     1861 atgtgagaat ctttccgaag acaggacaag gagactaatt tatgaagaac ggctttcgtt
     1921 gtttcgatag tgacatgcac gtttacgacg ctgcaaatct gtacgccaga tacatgaatc
     1981 caaagtgggg cgaccgaatt ccgcgtggcg aaagaaacgg taagcacggc agggtcgaat
     2041 tttccatcgc cggcggcaaa gagacgttac gcggcatcac cgatgtgatc gatcacggtc
     2101 agaagcaggt ggccgatcgc tatgactttg ccgtcgcgcg ggaatatgac gcactttcgc
     2161 aagtcgaagc gatggatcgc gagggtctcg atgtcgcagt gctcttccgg acttcacccc
     2221 tgcattgcga cgaaactctg gaaccagagt acgccaatga tttgtgtaga gcgtggaata
     2281 attggattgc tgatttttgc aacgtaaatc cgcagaggct caaggcctcg gcattgatta
     2341 ctttgcatga tgtcgagctc gcggtcgagg aagtgcgccg tgttgtccag cagctcggga
     2401 ttcgggccct gtcgctttgt ccggagccga tcaacggtcg gagaattcat gatcgctgtt
     2461 tcgatccgct ttgggcggag atcgaaaact tgggcgttgc actctgcttc catccgccgg
     2521 cgcgacccaa gcaggagcaa atagcaaaca ggtttttcgg ccacccgaac gcgaacattg
     2581 tcgcgctcgc gctgcgcaat ccgctcgaat tgattcaagc tgtcgcaagt ttttgcgctg
     2641 gcggtgtctt ggagaagttt cccaagctcc gcgttgcatt ccttgagggc aactgcgctt
     2701 ggctgccatg gcttctttat cggctcgacg aacgggccaa gctgcatggc ccgttggcgg
     2761 atgtgccgct gtcgcgcaag ccgagcgatt actttctcga gcagtgcttt atttcggttg
     2821 atcccgatga atatctcgtt acggatgtca tcaaacgaat cggcgacaat aatctcgtgg
     2881 tctccaccga ctatccgcac atcgacgcgc attttcccca tgccatcgac gaatttctgg
     2941 aaatcgaggg gttgagccag agcagccggc gcaaaattct ttgggacaat tgtgccaggc
     3001 tgtataac
//
LOCUS       DASULZ010000730         5444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1403624, whole genome shotgun sequence.
ACCESSION   DASULZ010000730 DASULZ010000000
VERSION     DASULZ010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403624"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VFY96_15030"
     CDS             complement(<1..195)
                     /locus_tag="VFY96_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607837.1"
                     /translation="MLRIAVPDLISNSYFPIIAAVEMGFFKEEGFDASVDLLFPIPKT
                     FEALRDGALDFVVGSAHATLL"
     gene            complement(261..611)
                     /locus_tag="VFY96_15035"
     CDS             complement(261..611)
                     /locus_tag="VFY96_15035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607838.1"
                     /translation="MKIVRLYTGTDSKSHFEDVELQFGGNQPMLTTDSRPATSAVFRC
                     APPGLVIDRHPAPRRQFLVTLSGSWEIEASNGVKRTFKTGDVMLADDTTGEGHISRVL
                     GTEPHIFMTVPLAD"
     gene            complement(656..1279)
                     /locus_tag="VFY96_15040"
     CDS             complement(656..1279)
                     /locus_tag="VFY96_15040"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX5607839.1"
                     /translation="MTRLGASYRTRTAAWLAFVAYLLTPSYFPRAADEQSFAGQLLVA
                     TDEMRDPRFIETIIYIVKDNSEGTFGLVINRPLASGPIDDLLKGFGAEPLSSKREVIV
                     HYGGPVSGLQGFVLHSDDMLLESSLRVRDGLAMTADVKMLEAIASAKGPGQFLIMLGY
                     AGWAPGQLEAEIKGNAWFVLPADKTLIFGLDADKKWRLAVDRRQTPL"
     gene            complement(1308..1739)
                     /locus_tag="VFY96_15045"
     CDS             complement(1308..1739)
                     /locus_tag="VFY96_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HEX5607840.1"
                     /translation="MTEFVPVQIDHAREFLRKHHWGILATRRKNGNLQMSPVTVGLDA
                     QGRAIISSRETAYKVNNLRRDSRAALCAITSSFHGEGWVQINGSAEIISLPDALDTLV
                     YLQRQVYGEHKSWPEFHERMAREKRVIIRIAVDTVGPQVRG"
     gene            2151..2840
                     /locus_tag="VFY96_15050"
     CDS             2151..2840
                     /locus_tag="VFY96_15050"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX5607841.1"
                     /translation="MAMTVPQMALPEVLAIFPLTGVMLLPGTVLPLHIFEPRYRAMVE
                     DALETNRVFGMIQPVAPQDDNRGPQPGADNLSPDLYKVGCVGYIEKWEKLPDGRFFLQ
                     LKGVNRFRFTEELELQRGYRRVKAQYQEFSDAGAEQNWHCDRAEVLSALASYGKAHGL
                     QVRPEQAEKFSDLELVNLLGVSLPFHPAEKQALLEAPGLKEREQMLINLLQLGAAPLD
                     TGEQTPPRTLN"
     gene            3022..3663
                     /locus_tag="VFY96_15055"
     CDS             3022..3663
                     /locus_tag="VFY96_15055"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX5607842.1"
                     /translation="MQRTQVIIVRHGQTEWNTRGIRQGHLDSELTEKGIIQAKALAQR
                     LARENFAALYCSDLGRAVQTAKEIADITGHEIVTDARLRERSLGIFQGLSGEEIKAKH
                     PAEYKLHRTLGPNYVIPGGESVRQQVDRNVTCLNELAGKHQGEQIVVVTHGGVVSGFF
                     RHTLAIPLEAPRRFEFVNAGLNVFFHEAGDWFLLTWGDISHLAPGAASEGDDP"
     gene            complement(4146..>5444)
                     /locus_tag="VFY96_15060"
     CDS             complement(4146..>5444)
                     /locus_tag="VFY96_15060"
                     /inference="COORDINATES: protein motif:HMM:NF014489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607843.1"
                     /translation="EHKENPWYWLKEGTKRIKKTRLRYHGGMHSAFEKTPHCILKLLV
                     ERLVSYGLTLTRTSNCWNDYTAFKEEIEDLKKNGMDTVANLIYSVSPRHTDEYYARKA
                     SEAAAAKPWRICFKDVGGLLTPERARTLIPVVLKAAGNIPVEFHAHCNSGQAPLNYLE
                     GVKLGMRILHTAIPPLANGSSQPSVFNIANNLKALGYAPQVDLRPLEPVVRHFTAVAK
                     KDGLPIGRPMEFDQSLYGHQVPGGMISNMRHQLKIVGMEHKMEAALEEAARVRADFGY
                     PIMVTPLSQFVGTQAAINVIVGERYKEVPDQNIQYALGIWGKEAVEVMDPNVRDKILS
                     RPRAKEWTHWEQPEPTLHEMRQKYGVNLSDEDLILYYFAGEDYVKALPDHGKPREYLD
                     ATQPLVKIIEQLSQRKESNQVFIKRPGFSVRMEKRSSASA"
BASE COUNT         1173 a         1493 c         1543 g         1235 t
ORIGIN      
        1 cagcaacgtt gcgtgggcgg agcccacgac aaaatcgaga gcgccgtcac gcaaagcctc
       61 gaaagttttt ggtatcggaa atagcagatc gaccgaggca tcgaatcctt cttctttgaa
      121 aaaccccatc tcgacggcgg caatgatcgg gaaataggag ttcgagatca gatccggaac
      181 ggcaatccga agcattttct ctcctgtagc agcttgaacg atatgaacgg cttgaacgtt
      241 tagaaccgtt tggcggcaat ttaatcggcc agcggcaccg tcataaaaat gtgcggctcg
      301 gtgccgagga cgcgcgagat gtgtccttcg ccagttgtat cgtccgcaag catcacgtcc
      361 ccggttttga acgttcgctt gacgccgttg cttgcttcaa tttcccagga accggaaaga
      421 gtcacaagaa attggcggcg tggtgcggga tggcggtcaa tcacaagtcc tggcggtgca
      481 cagcgaaaca ccgcgctggt ggccggacga gagtcggtgg tcagcatcgg ctgattgccg
      541 ccaaactgga gttcgacgtc ctcaaagtgt gatttgctat ccgtgcctgt gtagagtcta
      601 acaatcttca tcgctcctcc tggtggaatt cgttctgatc gagaacgacg agagtttaca
      661 gcggcgtttg tcgtctatcc acggctaaac gccatttttt gtcggcgtcc agaccgaaga
      721 tcagcgtctt gtcggccggt aggacaaacc atgcgttgcc tttgatttcc gcctcaagct
      781 gtccgggcgc ccagccggcg tatcccagca tgatcaaaaa ctgtcctggc ccttttgccg
      841 atgcgatcgc ctcgagcatt tttacgtccg cggtcatggc cagtccgtcc ctgacgcgca
      901 gcgaactctc cagcaacatg tcatcgctgt gcagcacaaa accttgtagg ccgctgacgg
      961 gcccgccgta atggacgatg acctcgcgtt tcgatgatag tggctccgcg ccgaaacctt
     1021 tgagtagatc gtcgatcggg ccactggcta gcggccgatt gatgaccagt ccaaacgttc
     1081 cttcggaatt gtctttaacg atgtaaataa tcgtctcgat aaaacggggg tcgcgcattt
     1141 cgtccgtcgc aaccaagagc tgtccggcga aactttgctc gtccgcggct ctcgggaagt
     1201 aggatggtgt cagcaagtaa gcgacgaagg cgagccacgc cgccgttctc gtgcgatacg
     1261 atgcgccaag ccgtgtcatc gctctattga ctggtagata gatgcagtca gccgcgaacc
     1321 tgcggtccga cagtatcgac tgcaatgcga atgatgacgc gcttctcgcg cgccattctc
     1381 tcatgaaatt ccggccagct cttgtgctcg ccatagacct gacgttgaag atagaccaaa
     1441 gtgtcgagag cgtccggcaa cgagatgatt tcggcgctcc cgttgatctg cacccagcct
     1501 tcgccatgaa aggagctggt aatcgcgcac agcgcggcgc gcgaatcgcg ccgcaaatta
     1561 ttgaccttgt aagccgtctc ccggctgctg atgatcgctc tgccctgagc atcaagaccg
     1621 acagtgaccg gcgacatttg cagattgccg tttttgcgcc gcgtggcgag aatcccccag
     1681 tgatgcttgc gcagaaactc tctggcgtgg tcgatctgga ccggaacgaa ctctgtcatt
     1741 acggattgcc tcgtcgaagc tcatgaattg cgtccctcga cactagaata gccgtcgagc
     1801 gatttgcaag agccgttgca gcttgagtgt gcttgagcat ctctttctcg cctactgaaa
     1861 gtaaattgtt tgaaccatca cccggtgttt tggcgtaatg ccgggtaaaa atcggcatcc
     1921 agaagcgttc gacagccggg attccagctc gccctacccc ggactggatg cggggttgcc
     1981 cggaatgatt ttgctgtgcg cgtcgatgat aggccagaaa atttcttgtg ctgcgctcgg
     2041 agctatttca aaattgcttt ggactcgaag ccaaactgcc agtcgcaagt tgcgaacctt
     2101 gaccgcctca gacggttgcc ctataaagca aacatgaatg aggcatgatc atggctatga
     2161 cagttccaca aatggcgctt ccggaagtgc tggcgatttt tccgctgacc ggagtgatgt
     2221 tgctgccagg gacggtgttg ccgctacaca tcttcgaacc gcgctatcgg gccatggttg
     2281 aggatgcgct cgagaccaac agagtattcg gcatgattca gccagtggcg ccgcaggacg
     2341 acaaccgcgg gccgcagccg ggcgccgaca atttgagtcc ggatctctat aaggtcggct
     2401 gcgttggcta tatcgaaaaa tgggagaagc ttcccgacgg cagatttttt cttcagctca
     2461 aaggcgtcaa tcgatttcgt ttcaccgaag agctggaatt gcagcgcggt tatcggcgcg
     2521 tgaaagccca gtatcaagaa ttttccgacg ccggtgcaga gcaaaactgg cactgcgacc
     2581 gagctgaggt gttatcggcg ctggcgagct acggcaaagc tcacggactg caagtgcgtc
     2641 cggagcaggc agaaaaattt tctgatttgg aattagtaaa tcttctcggt gtttcgttgc
     2701 catttcatcc cgctgaaaag caggcgctcc tggaagcccc cggcctcaag gaacgcgaac
     2761 agatgctgat caatctcctc cagcttggtg ccgcgccgct ggatacgggc gaacagactc
     2821 cgccccgcac cttgaactga tttagtcaac aatcgcgcga gcgtgaaaac gctcttttcg
     2881 cccactctca gctgctcact tccgcaggac cgaacagcat cggctattcg cgtggttttt
     2941 tgagcacgag cgtgagagtg caaagcaaaa agctcgaagc acagttgact tttttcctgg
     3001 tcccgtgcga ctaaggcact catgcagcgc acgcaagtga ttattgttcg acatggccag
     3061 accgaatgga atactcgcgg gattcgtcaa ggacatctcg atagtgagct gaccgagaag
     3121 ggaatcattc aggccaaagc gctggcgcag cggttggcgc gcgaaaattt tgcggcgctc
     3181 tactgcagcg atctggggcg cgccgtgcaa acagcaaaag agatagccga tatcacgggc
     3241 catgagatcg tcaccgatgc tcggttacgc gagcgtagct taggaatttt ccaaggactc
     3301 agcggcgagg aaattaaagc gaagcatccg gccgaatata aattgcatcg cactttagga
     3361 cccaattacg tgatcccggg cggcgaaagc gttcgccaac aggtcgacag aaacgtgact
     3421 tgcctgaacg agcttgccgg caaacatcaa ggcgagcaaa ttgtcgtggt cacccacggc
     3481 ggcgtcgtca gcggcttttt tcgccacacc cttgccatac cgctcgaagc gccgaggcga
     3541 ttcgaattcg tcaacgccgg attgaatgtt tttttccatg aagcaggcga ttggttcctt
     3601 ctcacctggg gcgatatcag ccatctggcg ccgggtgcag caagcgaagg ggacgatccc
     3661 taaaacgctg ctgaaaaagg tcatctgctt cgttgcgctc gtccgactca ctccaacgta
     3721 aacacatagg tatcgcagcg cttgtcctga ggcttaccga agggcacacc catcctacag
     3781 ttgaccatag gttcggggtg agagcctgtc ctgagcaaag acgaaggatc gatcttcgcg
     3841 cgcctcgcat atgagccttt ttgagcagcg tttattaaga gacttgttgg actgacgccg
     3901 cgttccaagt cgttcgagtc gtcagtcctt cgatacgaac cttcagattt acttaggatg
     3961 atcggatgaa accgattccc gagtagcgcg gagcgcgtat ccttcgatac ggcagcgcca
     4021 ctcgctgcct accgaggacg gtcggataag atgccgcgtc gcgcgtatcg agggacgctt
     4081 gttcaagact ttgaagcggt cgagcccgtt aacggtggac gctagcaaga ctcgaacgaa
     4141 agcgctcacg cgctcgcgct tgagcgcttc tccattctga cggaaaagcc ggggcgttta
     4201 atgaagacct ggttcgattc tttgcgttgc gaaagttgct cgatgatttt aaccagcggc
     4261 tgggtggcat cgaggtattc gcgcggtttg ccgtgatcgg gcagcgcttt aacgtaatct
     4321 tcgcctgcga aatagtacaa tatcaagtcc tcgtcggaga gattcacgcc gtacttctgg
     4381 cgcatctcgt gtagtgtcgg ctccggctgc tcccagtgcg tccattcctt ggcgcgcggc
     4441 cggctgagaa ttttgtctct gacgttgggg tccatcactt cgacggcttc tttgccccaa
     4501 atgccgagcg cgtattggat attctgatcg ggcacttcct tgtagcgctc gccgacgatc
     4561 acgttgatcg cggcctgtgt gccgacgaac tgcgaaagcg gcgtgaccat gatcggatag
     4621 ccgaagtcgg cgcgcacgcg ggcggcttcc tcaagcgcag cttccatctt gtgctccatg
     4681 ccgacgattt tgagctgatg gcgcatattg gaaatcatgc cgccgggaac ctgatgaccg
     4741 taaagcgatt gatcgaactc catcggcctg ccgatgggca gtccgtcttt tttcgcgacg
     4801 gcagtaaaat ggcgcaccac gggctcaagc ggtctcaaat ccacctgcgg cgcatagccg
     4861 agcgctttca ggttattcgc gatgttgaag accgaaggct gcgacgatcc gttggcgagc
     4921 ggcggaatcg ccgtgtgtaa gatgcgcatt ccgagcttga cgccctctaa ataattgagc
     4981 ggcgcttggc cgctgttgca atgggcgtgg aattccaccg gaatattgcc ggcggctttg
     5041 agaaccaccg ggatgagcgt gcgcgcgcgt tcgggcgtca gcagcccgcc gacatctttg
     5101 aagcagatgc gccagggctt ggccgcagcg gcttcgctcg ccttacgggc gtaatattcg
     5161 tcggtatggc gcggcgaaac ggagtaaatc agattggcca ccgtatccat gccgttcttt
     5221 ttcagatcct caatctcttc tttgaacgcg gtgtaatcgt tccagcaatt tgacgtgcgg
     5281 gtcaatgtga ggccatagga aaccagccgc tcgacgagaa gcttgagaat gcagtgcggc
     5341 gttttctcga acgcgctgtg catcccgccg tgatagcgca agcgcgtttt cttgatccgc
     5401 ttcgtgcctt ctttgagcca gtaccaggga ttttctttgt gctc
//
LOCUS       DASULZ010000731         2464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1199862, whole genome shotgun sequence.
ACCESSION   DASULZ010000731 DASULZ010000000
VERSION     DASULZ010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199862"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VFY96_15065"
     CDS             complement(<1..427)
                     /locus_tag="VFY96_15065"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HEX5607844.1"
                     /translation="MENQKNYRITKHQSELAVRVVGNSQADLFANSALTLFDVMVTDV
                     EKIDCKESIPLEVEGTDRDDLMVNWVRELLYLYQGSGYLLKEFLIREVKDTLVKADVR
                     GEKIDPDRHEIQQEIAAVASHKSRMQKTGNQWTAHLIFEI"
     gene            complement(741..1490)
                     /gene="larB"
                     /locus_tag="VFY96_15070"
     CDS             complement(741..1490)
                     /gene="larB"
                     /locus_tag="VFY96_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEX5607845.1"
                     /translation="MDSERLADLLEQVRRNQVSVAEAMARLRHLPFEDLGFAKIDHHR
                     ALRQGFPEVVMGQGKDAKQLAAIVQAMRQKRNNVLVTRLDSAKAAQLKRLRTGLTFHP
                     VARAATWHGKGIKITGKGTVLVVCAGTSDIAVAEEAVVTSEIMGNRVDRLYDVGVAGI
                     HRLLGNRARLDDASVLIVVAGMEGALPSVVGGLVAKPVIAVPTSIGYGASFDGLAALL
                     GMLNSCAAGVTVVNIDNGFGAGFAASLINRL"
     gene            complement(1490..>2464)
                     /gene="thiL"
                     /locus_tag="VFY96_15075"
     CDS             complement(1490..>2464)
                     /gene="thiL"
                     /locus_tag="VFY96_15075"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX5607846.1"
                     /translation="IGDDAAWIVNQKGSCLVTADLLIEGVHFDLRWTSLFDLGHKALA
                     VNLSDIAAMGGVPAYVILSLGLPPDLDSTQIDAFYRGLQALARRSGVVLVGGDTNAAV
                     TLIVSVCVIGHAPYAPVRRDGARAGDDIYVSGTLGDAALGLKLLQGGLSGSKKNRTVA
                     KLLTRHHRPEPRLVLGVLLAKSRLATAMIDVSDGLLQDLGHVCKASGVGATISEERVP
                     LSAAFRAVAKSGETAAALTGGEDYELLFCGRPQDRTRIEKISQRAGVSVTRIGVCAPR
                     AKGIAVIDKSGQLIPFRIRGHDHFAKKDFQSIKTSSHYVPKAKFTRTR"
BASE COUNT          567 a          699 c          642 g          556 t
ORIGIN      
        1 aaatttcaaa aatcaaatgc gcggtccact gattgccggt tttttgcatg cgactcttgt
       61 gagaggcaac ggcggcaatt tcctgctgga tttcgtggcg atcgggatcg attttttctc
      121 ctcgaacatc ggctttgacc agggtatctt taacctcgcg gatgagaaac tctttaagca
      181 aatagccgct gccctggtaa agataaagca gctcccgaac ccaattaacc atcaagtcat
      241 ctcggtctgt tccctcgact tccagaggaa tggattcctt gcagtcgatc ttttccacgt
      301 ccgtgaccat gacatcgaaa agcgtcagcg ccgaattggc aaaaagatcc gcttgtgaat
      361 tgccaacaac tctcaccgcc aattcagatt gatgcttagt aatacgataa ttcttctgat
      421 tttccatgat tatcgatttt gagtggaggg ctgttcgtgg gcgttctgca agatgctaag
      481 ctcgtgccgg cctgtgaaat catcttccaa gatttgccgg atttccgggc gggcataaca
      541 ttgaacacat gtcagaacgt cgatgggtcg aatcaatttg cggtatctga gcggtggcgc
      601 atcaggcttg cgactaacga aaaactccca ttcagtctcg ccggtgtgaa gcaagtaaaa
      661 gtcaacttta aaagtccttt gtgtgtgtac cgtaccaaaa acttctctgc cgcagtgaaa
      721 acactgcatt tgactatcat ttatagccgg tttatgaggc tcgccgcaaa cccggcgccg
      781 aagccattat cgatattgac taccgtcact ccggccgcac aagagttgag catcccgagg
      841 agtgcggcga gaccgtcaaa actcgcgccg tagccgatgc tggtcggcac cgcgatcacc
      901 ggtttagcga ctaggccgcc cacgacgctg ggcaacgcgc cttccattcc ggcaacaacg
      961 atcagcacgg aagcatcatc cagacgagcg cggttgccca aaaggcggtg aatgccggcg
     1021 acgccgacat cataaagacg atcgactcga ttacccatta tttctgacgt gacaaccgcc
     1081 tcttcggcca ctgcaatatc ggaggtgccg gcgcatacca ccagcacagt gcctttgccg
     1141 gtaattttga ttcccttccc atgccaggtc gccgctcggg ccacaggatg aaacgtcagt
     1201 ccggtccgta aacgctttag ctgcgccgct ttggcagagt caagacgggt gacgagaaca
     1261 ttgtttcttt tctgacgcat cgcctgcacg atcgccgcga gctgtttagc atctttgcct
     1321 tggcccatca ctacttcggg gaaaccctga cgcagtgctc gatgatgatc gattttggca
     1381 aagccgagat cttcgaaagg aagatgacgt aagcgggcca tcgcctcggc gacgctcacc
     1441 tggttacgcc gcacctgctc cagcaaatca gctagccgct cggagtccat tatcgagttc
     1501 tagtgaactt cgcctttgga acgtaatgcg acgaagtttt tatgctctga aagtcttttt
     1561 ttgcgaaatg gtcgtggccg cggatccgaa acgggataag ttgcccagat ttgtcgataa
     1621 cggcaattcc tttagctctc ggtgcacaaa cgccgatgcg tgtgactgac acaccggcgc
     1681 gctgcgaaat cttttcaatg cgcgttcggt cttgtggccg gccacaaaat aaaagctcgt
     1741 agtcttcgcc gccagtcaat gcggcggcag tttcgccgct cttggcgacg gctcggaagg
     1801 cggccgaaag cggcactcgt tcctccgaaa tcgtcgcccc gacgccgctc gctttacaaa
     1861 catgccccag gtcttgcaat aagccgtcgc tcacgtcgat catcgctgtg gcgagccggc
     1921 ttttggctag aagcacgcca agaacaagtc tcggctcggg ccggtggtgg cgcgtaagaa
     1981 gcttggcaac ggtgcggttt tttttggacc cactcaggcc gccttgcaga agcttgagcc
     2041 cgagtgccgc atcgccgagt gtgccgctga cataaatgtc gtctcccgct cgggcgccgt
     2101 ctcgacggac cggcgcatag ggtgcatgac cgatcacgca aacagaaaca atcagcgtga
     2161 ccgcggcgtt ggtatcgcca cccaccaaga cgacgcccga tcgacgcgcg agggcttgca
     2221 acccgcgata aaacgcatcg atttgtgtgc tatccaaatc gggcggcagc ccgagggaca
     2281 aaatcacata tgccggcacc ccgcccatcg cggcaatatc actgagattg acagctagcg
     2341 ctttgtgacc gagatcgaac agagaagtcc aacgcaagtc gaaatggacg ccttcaatca
     2401 agagatccgc ggttaccaga cacgagcctt tttggtttac gatccatgcg gcatcatcgc
     2461 cgat
//
LOCUS       DASULZ010000732         2971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_489498, whole genome shotgun sequence.
ACCESSION   DASULZ010000732 DASULZ010000000
VERSION     DASULZ010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489498"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VFY96_15080"
     CDS             complement(<1..909)
                     /locus_tag="VFY96_15080"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5607847.1"
                     /translation="MIFYLCAVVAIFVISLAEPGFAQNRQSTLAALTKLAPAERQARI
                     LEGAKKENGLVWYSSTTAEDALALTKKFHEHHPNIQINHFRSSSEKLLERILAESRAN
                     AFKADIVSLPELELSIMIKRKLLLRYEPLENSLYPPESKDPHGYWTGLYTSAWVPAHN
                     TKQVAKDAAPKSYKDLLNPKWKGGIAMDNEPYNWYVVSLRYLESRDGREAALDFMKKL
                     AAQQIQWRKGHSLIGQLMSAGEFPLAAELQVHTVERAKAQGAPIDWTVLDGVIPISKV
                     GAAITSTGANVYTSALFYDFLLSRPGM"
     gene            1049..1579
                     /locus_tag="VFY96_15085"
     CDS             1049..1579
                     /locus_tag="VFY96_15085"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX5607848.1"
                     /translation="MIIEYKGKAPRISPSAFVAPTAVIIGDVIVGDEASIWWGAVIRS
                     DQNQNPIRVGARTSIQDNCVLHSGEAPTIIEDDCTIGHGAIMEGPLIKRGAIIGMNVT
                     LLENVEIGEEALIAAGSVVVPHTKIPPRVLAAGAPAKVKKELAGEALHWIKMGSSTYV
                     NLCKSYKGGDYKVIVS"
     gene            1703..2617
                     /locus_tag="VFY96_15090"
     CDS             1703..2617
                     /locus_tag="VFY96_15090"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX5607849.1"
                     /translation="MRIILIGQAAFAEKSLEKIVSKGEEIVAVYCPPDPPSGKPDPVK
                     QKALSLGIPVRQHNSFKAPELREEFVALNADLTILAFVSQIVPEHVFSAPRLGSICFH
                     PSLLPRYRGASAINWALIKGETVTGLTLFWVDRGIDTGPILLQKEVPVDPDDTTGSLY
                     FNKIFPLGIEAIGEAVDLIKKGNPPRIIQDESKATYDPPCRDEHARIDWSKPATEVYN
                     LIRGCDPQPGAHTTYRGKQLRIFDARLQRGATGSLPGQVTDIGIDEITIALNGGSLAV
                     KRIRGEGAKITAAEFAQEAGVEVGAELR"
     gene            2771..>2971
                     /locus_tag="VFY96_15095"
     CDS             2771..>2971
                     /locus_tag="VFY96_15095"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX5607850.1"
                     /translation="MDASHSFAPLLIVLLIAFAVPIFLSRIKYLSIPIVVGEIVAGMI
                     VGRSGLSFVPPDDLLVGIFANFG"
BASE COUNT          687 a          828 c          814 g          642 t
ORIGIN      
        1 catgccgggg cgggacaaca gaaaatcata aaatagcgcc gaagtataaa cattcgcgcc
       61 ggtgctggta atcgctgcgc caactttgga aatcggaatt acgccgtcca acacggtcca
      121 atcgatgggc gcaccttgcg ctttggcacg ttccacggtg tgcacctgaa gctccgcggc
      181 gagcggaaac tcgcctgcag acatgagctg tccgatcagc gaatggccct tgcgccactg
      241 gatctgctgc gccgcgagtt ttttcatgaa atccagtgcc gcttctcttc cgtcccggct
      301 ctccaaataa cgtagcgaga ccacatacca attgtacggc tcgttatcca tcgcaatgcc
      361 gcccttccac tttggattga gcagatcctt gtagctcttc ggcgcagcat ccttggcgac
      421 ctgcttggtg ttatgcgcgg gtacccatgc ggaggtgtaa agtcccgtcc aataaccgtg
      481 cggatctttg ctctccggcg gatagagtga attttccagc ggctcgtagc gaagcaaaag
      541 tttccgcttg atcatgatcg aaagttcgag ctccggcagg ctgacgatat cggccttgaa
      601 ggcattggcg cgggattccg cgagaatcct ttccagcagt ttctcgctcg aagaccgaaa
      661 gtgattgatt tgaatgttgg gatgatgctc gtggaatttt ttcgtcaggg ctaaagcatc
      721 ctcggcggtc gtgctcgagt accagacgag cccattctct ttcttagcac cctcgagaat
      781 acgggcttgg cgctcggccg gagcaagttt cgtaagcgcc gccaaggtgg attggcgatt
      841 ttgcgcgaac cccggttcgg ctaaactaat gacaaagatc gcgaccactg cacacaaata
      901 aaaaatcatt ttccactcca atcctaaatc gtactgcgac tccctcgcct gctgcgggag
      961 acggtcaggg ttgagggacg tccgcctacc attattgatt tcgcgagtca cgtcaaatat
     1021 cgtggttcaa atctacggag gatgccgcat gattatcgag tacaagggta aagcgccgcg
     1081 tatttcaccg agcgcgtttg tcgcgccgac cgcggtgatc atcggcgatg tcatcgtcgg
     1141 cgatgaggcc agcatctggt ggggcgccgt gatccgatcc gaccagaatc aaaatcccat
     1201 cagggtcggc gcgcgtacca gcattcagga taattgcgtg ctccattccg gtgaagcgcc
     1261 gaccatcatc gaagacgatt gcaccatcgg gcatggcgcc attatggaag gcccgctgat
     1321 caagcgtggc gccatcatcg gcatgaacgt cacacttttg gagaatgtcg aaatcggcga
     1381 ggaggcactg atcgccgccg gcagcgtcgt cgtgccgcac accaaaattc ctccccgggt
     1441 cttagccgcc ggcgcaccgg caaaggtcaa gaaagagctc gccggagaag ctttgcattg
     1501 gatcaaaatg ggctcttcga catacgtgaa cctgtgcaaa agctataagg gcggcgacta
     1561 caaagtgatc gtaagttaag tgagttgcaa acggttcaag ccgtttcagt cgttcaaaac
     1621 gtcgtcagat atgaactttt gcgcttggaa cgccatccca aaccaactca cgagactgca
     1681 aattcactag agaaggacaa cgatgcgcat cattctgatc ggtcaagcgg ctttcgccga
     1741 aaagagtttg gaaaaaattg tgagcaaggg cgaggaaatc gtcgcggtct actgcccgcc
     1801 ggatccgccg agcggaaaac cggatccggt caaacagaaa gcgctcagtc tcggcatccc
     1861 ggtgcgccaa cataactcct tcaaggctcc cgaacttcgt gaagagttcg tcgcgctgaa
     1921 cgcggatttg acgattctgg cttttgtttc ccagatcgtt cccgagcacg tctttagcgc
     1981 cccccgactc ggcagcatct gctttcatcc ctctttgttg ccgagatatc gcggcgcgag
     2041 cgccatcaac tgggcactga tcaaaggcga aaccgtgacc gggctcacgt tgttctgggt
     2101 tgatcgcggc attgataccg gcccgatcct gctgcaaaaa gaagtgccgg ttgatcccga
     2161 cgatacgacg ggctctctgt acttcaacaa aatatttccg ctcggcatcg aagcgatagg
     2221 tgaagctgtg gatttgatca aaaagggaaa tccgccgcgg atcattcagg acgaatccaa
     2281 agcgacctac gatccgccct gccgcgatga gcatgcgaga atcgattggt ccaagccagc
     2341 aacggaagtt tataatttga ttcgggggtg tgatccccag cccggcgcac acacgaccta
     2401 tcgcggcaaa caactgcgca tcttcgatgc ccggctgcaa cgcggcgcca ccggctctct
     2461 gccgggtcag gtgacggata tcggaatcga tgaaattacg attgcgctga atggcggcag
     2521 ccttgccgta aaaagaatcc gcggcgaagg cgctaaaata accgcggcgg aattcgccca
     2581 agaggcggga gtagaagtcg gcgctgagtt gcgctaggcc cattggctgc caaacagctt
     2641 gcaaataatt acctttaagc cagctcctga agcgcgccgc cgaaccatat tacaagcgtc
     2701 ggcgcgagca gcgcatcgat agagcggcca acggcgcgcc agcaagacta aatagatcga
     2761 gcccgcaaaa atggacgcca gccattcctt cgcgccattg ctgatcgttc tgctgatcgc
     2821 gtttgcagtg ccgatttttc tctcgcgcat caaatacctt tcaattccca tcgtcgtagg
     2881 agaaattgtc gctggaatga tcgtcgggcg gagcggtttg tcgttcgtgc cgcccgatga
     2941 tctgctggtc ggaatttttg cgaacttcgg c
//
LOCUS       DASULZ010000733         3395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1097053, whole genome shotgun sequence.
ACCESSION   DASULZ010000733 DASULZ010000000
VERSION     DASULZ010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097053"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VFY96_15100"
     CDS             complement(<1..276)
                     /locus_tag="VFY96_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX5607851.1"
                     /translation="MSEIVKITRISGTAVPIRGNDIDTDRIIPARYLKEPTFARMGEY
                     PFFDERFDTAKNKKDHPFNDPEYEGASILFVNKNFGCGSSREHAPQAL"
     gene            complement(286..1683)
                     /gene="leuC"
                     /locus_tag="VFY96_15105"
     CDS             complement(286..1683)
                     /gene="leuC"
                     /locus_tag="VFY96_15105"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012598252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX5607852.1"
                     /translation="MAGKTLYDKIWQSHSVGTLPTGQTQLFIGLHLIHEITTAPAFDM
                     LREAGMDIAFPERTFATVDHIVPTDLRKRPFLDPEAEELTQVLEKNVAQFGVEFFGLE
                     SMNQGIVHVIGPQLGLTQPGMTLACGDSHTSTHGAFGTLAFGIGTSQVRDVLATQTLA
                     MDKLKVRRININGALGTGVYAKDVILYIIRRLGVAGGKGFAYEYGGAIIDGMNMEERM
                     TVCNMSIEGGALVGYVNPDQTTIDYLKGRPYAPKDAAFDRAVSYWKSIASDADAQYDD
                     VVNYHGSDIEPTVTWGINPGQSVGISERLPKPEEFADPDGAKKAYEHMGWLPGAPMLG
                     TPINVAFIGSCTNGRLADLRAAAKIVRGRKVNSNVRALVVPGSAEVKKVAESEGLHEI
                     FTDAGFQWREAGCSMCLAMNPDKLNGREISASSSNRNFIGRQGSPQGRTLLMSPAMVA
                     AAALNGKIVDVREYE"
     gene            complement(1751..3142)
                     /locus_tag="VFY96_15110"
     CDS             complement(1751..3142)
                     /locus_tag="VFY96_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607853.1"
                     /translation="MCDTADDGVGISNSVPAELKLRNRGLRTADIAELMPFARQLVNS
                     RENRDSHIGFVGYNKTKAGNRVLIAIDREYDLDVPHAVAAALREKGAHVDILVADMGD
                     PDRMFDYTDEVEVIMRREPWDENPRRWEGMPYIEDFALRAGYDLLIHGKGGPIPKTDF
                     RYEQIPWLRAEHFLQRSTTYPLDLHLLINKKTWDPIWEYGRGGRAHLTDAEGTDISWT
                     YWDEYYDGTRYGFSSTPRNGHLFGHPVPPIIAKEDAAGVVAGTTSHFSKAFPQVKVEL
                     EAGQIAKVAGGATYGNAWRSLLDESRATQYPCFPRPGLFYLWEVAIGTNPKIMRPTGI
                     DKHSSGGFEWERRRSGVIHMGFGTLWRSAEEKWAGENGLLYGHLHIHLLFSTFTITTK
                     AGREHTIIRNGRLTALDDHEVRKLAEKYGDPDDLLREDWIPQVPGITCAGSYDEYSRN
                     PGKWIYGRQEAQG"
     gene            complement(3212..>3395)
                     /locus_tag="VFY96_15115"
     CDS             complement(3212..>3395)
                     /locus_tag="VFY96_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="allantoinase"
                     /protein_id="HEX5607854.1"
                     /translation="ILYEESADEPKMLTLAMHCQMAFPATGKVYDECIKYAKSFPDLW
                     FAKRCEIVDWVKEKCL"
BASE COUNT          736 a          978 c          891 g          790 t
ORIGIN      
        1 gagcgcctga ggcgcatgct cgcgggaaga accgcagccg aaatttttgt tgacgaaaag
       61 gatcgacgca ccttcatatt caggatcgtt gaacgggtga tccttcttgt tctttgcagt
      121 atcgaaccgc tcgtcgaaaa acgggtactc gcccatgcgc gcgaacgtcg gttcttttaa
      181 atagcgggcg ggaatgatgc ggtcggtgtc aatatcattg ccccggatgg gcaccgcggt
      241 gccactgatc ctggtaatct ttacaatttc gctcatgctt cctcgttatt catactcacg
      301 tacatcgacg attttgccat tgagtgctgc cgccgccacc atcgccggac tcattagcaa
      361 ggtgcgtccc tgagggctcc cctgccggcc gataaagttg cggttcgagg atgatgctga
      421 gatctcgcgg ccattgagtt tgtccggatt catcgccaga cacatcgaac aacccgcctc
      481 gcgccattga aatcccgcat ctgtgaatat ttcgtgcaag ccttcactct cggccacttt
      541 tttcacttcc gccgagccag gcacaaccag cgcgcggacg ttggaattta cttttctgcc
      601 tctgacgatt tttgccgcgg cgcgcagatc cgcgagcctg ccgttagtac aggaaccgat
      661 gaatgcgaca ttgatcggtg tgccgagcat cggcgcgcca ggcagccagc ccatatgctc
      721 atatgctttc tttgccccgt cggggtcggc aaactcttca ggcttcggca gtctttccga
      781 aataccgacg gattgcccgg ggttgatgcc ccacgtaaca gttggctcga tatcgcttcc
      841 atggtaattc acgacgtcgt cgtactgagc atcggcgtcg gacgcaatcg atttccaata
      901 gctcaccgcc cgatcgaagg ccgcatcttt gggagcgtaa gggcgcccct taagatagtc
      961 gatggtcgtt tggtccggat ttacgtagcc tacgagggca ccgccttcaa tgctcatatt
     1021 gcagacggtc atgcgttctt ccatgttcat cccatcgatg atcgctccgc catattcgta
     1081 ggcgaagcct ttaccgcccg cgacaccaag ccgtcgaatg atataaagga ttacgtcttt
     1141 cgcataaaca cccgtgccga gcgcgccatt gatattgata cggcgcacct tgagtttgtc
     1201 catcgccaaa gtctgagtcg ccaagacatc acgcacctgg ctggtgccga tgccgaaagc
     1261 caacgtgcca aaagccccat gcgtgctcgt atggctgtcg ccgcatgcga gcgtcattcc
     1321 cggctgcgtt aaaccgagtt gcgggccgat cacgtgaaca atgccttgat tcatgctctc
     1381 caaaccgaaa aactcgacgc cgaattgcgc cacgtttttt tccagtacct gggtcaattc
     1441 ttctgcctcg ggatccagaa acgggcgttt gcgcaaatcg gtgggcacga tgtgatcgac
     1501 ggttgcaaag gtgcgctcgg gaaaagcgat atccataccc gcttcacgca acatgtcgaa
     1561 agcgggagcg gtggtgattt cgtggattaa atgaagcccg atgaagagtt gtgtctgacc
     1621 cgtcggcaac gtgccgacac tgtgagattg ccaaatctta tcgtacaggg tctttcccgc
     1681 cattaaaaaa ctctccttcg ccgggatcat atcggccgac atgttcgact tcaagcgacc
     1741 gctgattgct ttatccttga gcctcctgtc gaccataaat ccatttgccc ggattacgcg
     1801 agtactcatc gtaggacccg gcgcaggtaa ttcccggaac ctgtggaatc caatcctcac
     1861 gcaaaagatc gtcggggtcg ccgtacttct cggccagctt ccggacctca tgatcatcta
     1921 aagctgtgag tctgccatta cgaatgatcg tgtgctcccg tccggccttg gtggtgatcg
     1981 tgaaagtcga aaaaagtaaa tgaatgtgca aatggccata gagaagcccg ttttcaccgg
     2041 cccatttttc ttccgcggag cgccataatg tgccaaatcc catgtggatc acgcccgacc
     2101 ggcgccgctc ccattcgaaa ccgccggagg aatgtttgtc gatccccgtc gggcgcatga
     2161 ttttgggatt ggtgccgatc gcgacttccc agaggtagaa caagcccggt cgtggaaaac
     2221 aggggtactg tgtcgcgcga ctctcatcca gcaagcttcg ccaggcgttc ccgtaagttg
     2281 cgccgccggc aactttagcg atctgtccag cttccagttc gactttgacc tgcggaaagg
     2341 ctttggaaaa atggctggtg gtgccagcca caacccccgc ggcatcctct ttggcgatga
     2401 tcggtggcac cggatgaccg aagagatggc cattgcgcgg cgttgaggaa aaaccatagc
     2461 gcgtgccgtc gtagtattcg tcccagtagg tccaactgat atcggtacct tcggcatcgg
     2521 tgagatgagc gcgcccaccc cgtccgtact cccatatagg atcccaggtc tttttgttga
     2581 tcaacaagtg caaatcgagc ggataggtcg tcgaccgctg taaaaaatgt tcggcccgaa
     2641 gccacggaat ctgctcatag cgaaaatcag ttttgggaat cggaccgcct ttgccgtgga
     2701 tcaacaaatc gtagccggcg cgcagcgcga aatcttcgat atagggcatg ccttcccaac
     2761 ggcgaggatt ttcgtcccag ggctcgcgcc gcatgatgac ttcgacctcg tcggtataat
     2821 cgaacatgcg atccggatcg cccatgtcgg cgaccaaaat atcgacatgc gcgccctttt
     2881 cacgaagcgc agccgcgacg gcatgcggca catccaggtc gtactcgcga tcgatggcga
     2941 tcagaactcg attgccggct ttggtcttat tgtaaccgac aaagccgatg tgactgtcgc
     3001 ggttctcgcg tgagttgacg agctggcgcg cgaacggcat cagctcggca atatccgccg
     3061 tgcgaagccc gcggttacgt agcttcaatt ctgcgggaac agaattgctg ataccaacgc
     3121 catcatccgc tgtgtcacac atgcttcctc caggtgagca gaggcgtcag gcgtaagggg
     3181 aaccttagct cctcacccct tactccttac gctataaaca tttctctttc acccagtcca
     3241 cgatttcgca tcttttggca aaccaaagat ccggaaacga tttagcatat ttgatgcact
     3301 cgtcataaac tttgccggta gcgggaaaag ccatctggca gtgcatcgcc agggtcagca
     3361 tcttcggttc atcagcggac tcctcgtaaa gaata
//
LOCUS       DASULZ010000734         2957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_896032, whole genome shotgun sequence.
ACCESSION   DASULZ010000734 DASULZ010000000
VERSION     DASULZ010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896032"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..1285)
                     /locus_tag="VFY96_15120"
     CDS             complement(236..1285)
                     /locus_tag="VFY96_15120"
                     /inference="COORDINATES: protein motif:HMM:NF019003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HEX5607855.1"
                     /translation="MAKLLHYLNQFFAGIGGEDKASQGFLCMPKPVGPGALLSDLLKN
                     RDIEYATVVCGDNYFHENEEAVLTKLGEVMTRFAPDLFIAGPAFHAGRYGIACAKAAS
                     FALERWGVPAVTGLFEENPGTREIGRHVLVLQTGASAASMQSTLKRCADLATLILNHD
                     RDGLEAFKEEFCLKIPRRFTVQADAPDSVRALDMLLAKLRGGAFESEIPRIQAVPHPI
                     PAAIQDLSQARLAMVTEGGLVPMGNPDRLESSRGTKYFKYSIKERDDLKAGEFEAMHT
                     GYDTSTVNADPDRLIPVDAMRRLEREGNFARLHDYYYVTTGTGAMPAKMQELGIGIAE
                     EIFASGVNGVVLTAT"
     gene            complement(1295..2581)
                     /locus_tag="VFY96_15125"
     CDS             complement(1295..2581)
                     /locus_tag="VFY96_15125"
                     /inference="COORDINATES: protein motif:HMM:NF020896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HEX5607856.1"
                     /translation="MELELLDFHVDDIQWGERSSLVGRQLIVSLTEIQDHVKDLSQNI
                     KLTAKLARPGESKRIIHVLDTLMPIAKVDDERWTFPGIDHPARLVGTGRTERLRNVLV
                     TIAGRFPHAELMTPIEQPREGLIDMSGIGARYCHGSDSFHIILTLEGEAALSNLAFDQ
                     ALRYIGVRVARYLAGVGKSGAAPDTKKICLNRIDRKLPKVVLIYQVQSQIPLVRTFYY
                     GEEISKTLPSFVHPNEFIDGAVVSGTYKSERKIPTSLHCANPFIEEMLKRHGREIDFL
                     GVILSRGYNDSFEQKKKMGLWSARLARTLGADGAVAVMEGTGNTTVDFMQTVKACEEE
                     GIKTVAVLHEDNGARGYERPLVDHPREADAMISRGNRSEKLSLPPLDEVIGGAEIDLH
                     LKAKHDPHKPLFFDPTIFFGAHCQMGASGFRAAYES"
     gene            2709..>2957
                     /locus_tag="VFY96_15130"
     CDS             2709..>2957
                     /locus_tag="VFY96_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HEX5607857.1"
                     /translation="MKVLLVQPPVRDFYDTDVRLQPIGLAYLKAAVKKHLPHVEISIK
                     DYHGGCGRRTIAIPNELRDLMDYYPLADKSPFSTFHQYY"
BASE COUNT          628 a          882 c          763 g          684 t
ORIGIN      
        1 caaccggcgt ctgcaaaacc ttgagcgccg cgctcaacac ctgtgcgcgc cagttcacct
       61 catcctccgg cgaaagctcc ggatttccga cggggtagtg aaatttgttt ccggtcatga
      121 tccggtaggc gccgacctgc gttgccacgt tcggaaggca cgtgatgagc acgacaggta
      181 tgcctttccg ctcgatctct tttgcaatcg tagcgccgct acgcgtgccg gttcctcacg
      241 tcgccgtgag caccacgcca tttacgcccg aagcgaaaat ctcctcggca ataccgatgc
      301 cgagctcttg cattttagca ggcatggcgc ccgtgccggt cgtaacgtaa tagtaatcgt
      361 gaagtcgtgc gaaatttccc tcgcgctcca atcgccgcat cgcgtctacg ggaatgagcc
      421 ggtccggatc agcgttcacc gtggaggtat cataacccgt gtgcatggct tcgaactcgc
      481 cggctttcaa atcgtcgcgt tccttgatcg aatacttgaa atacttcgtt ccgcgcgagc
      541 tttccaggcg atcaggattg cccatcggga ccaaaccgcc ttcggttacc atcgcgagcc
      601 tcgcctgcga tagatcttga atcgccgcgg gaatcgggtg cggcacggct tggattctgg
      661 gaatctcgct ctcgaaagca ccgccgcgta atttcgcgag cagcatgtcc agcgctcgaa
      721 cactgtcggg agcgtccgct tgaaccgtga atcggcgtgg aattttgaga cagaactctt
      781 ctttgaaagc ctccagaccg tcacgatcgt gattcagtat gagcgtcgcg agatcagcgc
      841 agcgcttcag ggtggattgc atcgaggcgg ctgacgctcc ggtctgaagc accagcacgt
      901 gccggccgat ttcgcgtgtg ccgggatttt cttcgaacaa ccccgtcact gccggcacgc
      961 cccagcgctc gagcgcgaag ctcgcggctt tcgcgcaagc gattccatat cgtcccgcgt
     1021 ggaaggccgg accggcgatg aacagatctg gcgcgaaccg cgtcatcacc tcgcctagtt
     1081 ttgtcagcac agcttcttca ttctcgtgaa agtaattatc gccgcagacc acggttgcat
     1141 attcgatgtc gcgatttttc agcaggtcac tcaacagcgc tcccgggccg acgggctttg
     1201 gcatgcacaa aaatccctgg ctagccttgt cctcgccgcc gatcccggca aaaaattgat
     1261 tgagatagtg taatagcttt gccatttttt cgcctcagct ttcgtaagcg gcgcgaaagc
     1321 cgctcgcgcc catctggcag tgcgcgccga aaaaaatggt cgggtcaaaa aacagtggct
     1381 tgtgcggatc gtgcttggct ttcaagtgaa gatcaatctc tgcgcctccg atcacttcgt
     1441 cgagaggggg aagtgataat ttttccgagc ggttgccgcg gctgatcatg gcatcggctt
     1501 cgcgcggatg atccaccagc ggcctctcat aacccctggc gccattgtcc tcatgcagca
     1561 cggccacggt tttgattccc tcttcttcgc aagctttgac ggtctgcatg aaatcgacgg
     1621 tcgtattgcc ggtaccctcc atcaccgcaa ccgcgccgtc tgcgcccaag gttctggcca
     1681 gtctcgcgct ccacaaaccc attttctttt tctgctcgaa gctgtcgttg tatccacgcg
     1741 aaagaatcac gccgagaaaa tcaatttcgc ggccgtggcg ctttagcatt tcttcgataa
     1801 aggggttggc gcagtgaagc gatgtcggaa tctttcgttc gctcttatag gtgccgctca
     1861 ccaccgcgcc gtcgatgaac tcattgggat gaacgaacga gggcagcgtt ttcgagattt
     1921 cctcgccgta ataaaaggtc cgtaccagag gaatttggct ctgcacctga tagatcaaga
     1981 ccactttcgg aagcttcctg tcaattcgat tgagacaaat cttttttgtg tccggcgccg
     2041 cgccgctttt tccgactcca gcgagataac gcgcaacgcg taccccgata taccgaagcg
     2101 cctgatcgaa agctaaattc gaaagcgccg cttcgccttc gagcgtcaga atgatgtgaa
     2161 aagaatccga cccgtggcag taacgcgctc ctataccgct catatcgatc agcccctccc
     2221 ggggctgctc gatgggagtc attagctcgg catgaggaaa tcttccggca atcgtcacca
     2281 atacgttacg cagtcgctcg gtccgtccag ttccgaccaa ccgcgcgggg tgatcaatgc
     2341 cgggaaaagt ccagcgctcg tcatccactt tcgcgatcgg catcagcgta tccaaaacat
     2401 gaataattct tttcgactcg ccagggcgcg caagcttcgc ggtcagcttg atattttggc
     2461 taagatcctt gacgtggtct tgaatctcgg tgaggctgac aataagttgt cgtccaacaa
     2521 gcgaggatcg ctctccccat tggatatcgt ccacatgaaa atcgagaagc tctaattcca
     2581 caatttccgc tccggagagt tcgaggccaa gctggacggg actatactct tcagaaaatc
     2641 tccgtacaag tttggtccag actagaatgc gcgatccagc ttcacttgag aaaaactccc
     2701 gcacgaagtt gaaagtcttg ctggttcaac ctccggtccg agacttttac gacaccgacg
     2761 tgcgcctcca gcccatcggc ctcgcttacc tcaaagccgc cgtaaagaaa cacttgcctc
     2821 acgttgaaat cagcatcaaa gactaccacg gcggttgcgg tcgacggact atcgccattc
     2881 cgaatgagct gcgcgatctc atggattact atccgctggc cgacaagtcg cccttttcga
     2941 cgtttcatca gtactac
//
LOCUS       DASULZ010000735         3736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_692700, whole genome shotgun sequence.
ACCESSION   DASULZ010000735 DASULZ010000000
VERSION     DASULZ010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692700"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="VFY96_15135"
     CDS             complement(<1..958)
                     /locus_tag="VFY96_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEX5607858.1"
                     /translation="MKRFICIHGHFYQPPRENPWLEAVELQDSAAPYHDWNARITAEC
                     YASNSVSRILDEQNRIRKLVNNYAKMSFNFGPTLLSWLAEKSPAVYDAILAADRESVK
                     KFSGHGSAIAQPYNHMIMPLANRRDRITQIAWGIRDFETRFQRRPEGMWLPETAVDLE
                     TLELLADFGVRFTILAPHQAARTRRIGQSEWRELEDRGIDTNRAYRQRLPSGKSLALF
                     FYHGPIARAVAFEGLLSHGERFIQRLYGAFSAQDEQEQLVHIATDGESYGHHHRFGDM
                     ALAYALDRIEADSQTALTNYGEFLARHPPNDEVEIVEKSSWSC"
     gene            complement(1020..2264)
                     /gene="glgC"
                     /locus_tag="VFY96_15140"
     CDS             complement(1020..2264)
                     /gene="glgC"
                     /locus_tag="VFY96_15140"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008949276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEX5607859.1"
                     /translation="MYVLGVVLAGGRGERLYPLTQHRAKPAVPFGARYRIIDFVLSNF
                     VNSGILSIYVLTQFKAQSLLEHLDRGWRTSDFLGEHFVTPVPAQMRSGEDWYQGTADS
                     VYQNLYLLERHSPKLVAVFGADHIYRMNVRQMIDEHQKKNADVTVAALPVKIEEACQF
                     GVIEIDPDWRIVDFREKPKNPKSIPGEPNFALVSMGNYLFNNEILVDALSANAEKPES
                     THDFGRDILPELIGSQRVFAYDFRKNRVPIPSKGEEPSYWRDLGTIDAYYEASMDLCS
                     IDPSFNLYNRSWPLRTVGYFDPPAKFVFDWPDRQGMAVNSVVAEGTIISGSYVHNCVV
                     GRNVRIHSYSQIEESVIMDWVEIGRGCKIRHAIIDKANVIPSGTTIGYDTESDRERYK
                     ISPGGVVVLGRGPRKTSWVMNP"
     gene            complement(2287..>3736)
                     /gene="glgA"
                     /locus_tag="VFY96_15145"
     CDS             complement(2287..>3736)
                     /gene="glgA"
                     /locus_tag="VFY96_15145"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEX5607860.1"
                     /translation="LSPMKIALIASEVTPFAKTGGLADVLGSLSVVLEQLGNEVFVIA
                     PAYRSVLRGGFALQQTSMQFSVPISGRSVLAEVLETLIGNRVRIFFIRADQYFDREFL
                     YTTPAGDYPDNSERFVFFSRAALELLRQRPADVVHCHDWQTALMTVYLKTQAARYSEI
                     ARAKTVFTIHNLGFQGIFGPADWRLLDLDVVFFTPPYLEFYGNINYLKGALIFADKLT
                     TVSPSYAAEILTPEQGFGLEGVLRQRADDLVGILNGVDYQLWNPWTDPFLTDHYGENS
                     LSQKSACKENLRQIVGLPSREDSPVIGMISRLTPQKGVDLVEGILDALMARGVQLILL
                     ANGDVRYENFFRAAMARFPEQVAVRIGFDNPLAHQIEAGADIFLMPSLYEPCGLNQMF
                     SLKYGTIPVVRAVGGLKDTVQQCNVSARSGTGFLFGPYTADALLAALDECLLVYRNRP
                     AWSALQRRAMAMDFSWNRSAKNYTELYRQLVS"
BASE COUNT          844 a         1034 c         1047 g          811 t
ORIGIN      
        1 cgcagctcca ggagcttttc tcgacgattt ccacttcgtc attgggcgga tgtcgtgcga
       61 gaaattcacc gtaattcgtg agcgccgtct gcgagtcagc ctcgatgcgg tccagagcgt
      121 aggccaacgc catatcaccg aagcgatggt ggtgaccgta gctctcgcca tcggtagcga
      181 tgtgaaccaa ttgttcctgc tcgtcttgcg ccgaaaaagc gccgtacagt ctttgaatga
      241 agcgttcgcc gtgtgagagc agaccctcga aagcgacagc gcgggcgatc gggccgtggt
      301 agaaaaatag cgccagggat tttcccgagg ggagccgctg tcgataggcc cggtttgtgt
      361 cgatgcctcg atcctcaagc tcccgccact ccgactggcc aatccgacgc gtgcgtgcgg
      421 cttggtgcgg cgcgaggatt gtaaagcgca cgccaaaatc cgcgagcaac tcgagggtct
      481 cgaggtcgac cgccgtctcc ggcagccaca tcccttcggg cctgcgctgg aagcgggttt
      541 caaaatcacg aatgccccag gcaatctgcg tgatgcgatc acgccggttg gccagaggca
      601 tgatcatgtg attgtagggc tgcgcgatgg ccgatccatg gccggaaaat tttttaacgc
      661 tctcccgatc ggccgcgagg atcgcgtcgt aaacagcagg cgatttttct gccagccagg
      721 agagtaaggt gggaccaaag ttaaagctca tcttggcata attgttgacc agcttcctga
      781 tgcggttctg ctcgtcgaga attcgcgaca ccgaattcga tgcgtaacat tcggcggtaa
      841 tccgggcgtt ccaatcgtgg tatggcgccg ccgaatcttg caactccacc gcttcgagcc
      901 agggattctc tcgtggtggc tgataaaagt ggccgtgaat gcagataaac cgcttcatgc
      961 gtgatttcgg ctcggacagc tattcaatcg cgctcgtttc gcagcggagg agtgccagcc
     1021 tacggattca taacccagct agttttacgc gggccccgcc ccaatacgac cacgccaccg
     1081 ggggagattt tataccgttc ccggtcgctt tccgtgtcgt aaccgattgt cgtgccggag
     1141 ggaatcacat tggctttgtc aatgatcgca tgacgaattt tgcagccgcg cccgatctcc
     1201 acccagtcca tgatcactga ctcttcgatc tgactataac tatgaatgcg cacgtttctt
     1261 cccaccacac aattatgcac gtagctgccg ctgatgatcg ttccttctgc aaccacggag
     1321 ttgaccgcca tgccttggcg atctggccaa tcaaagacaa acttcgccgg cggatcaaaa
     1381 tagccaaccg tgcgcagcgg ccaactgcgg ttgtagaggt tgaatgatgg gtcgatggag
     1441 caaagatcca tgctcgcttc gtaataggcg tcaatggtgc cgagatcacg ccagtaactc
     1501 ggctcttcgc ctttcgacgg aatcggcacg cgatttttgc ggaaatcata ggcaaagacg
     1561 cgctggcttc cgatcaactc tggcaagatg tcgcgcccga aatcgtgcgt gctttccggc
     1621 ttttcagcgt tagcgctgag cgcgtccaca aggatctcgt tattaaaaag ataattgccc
     1681 attgagacca gagcaaaatt gggctcgccg ggaatggatt tgggattttt cggcttttca
     1741 cgaaaatcga cgatgcgcca gtcggggtcg atttcgatga cgccaaactg gcaggcttct
     1801 tcaatcttca cgggtagggc tgcgacggta acgtcggcgt tcttcttttg gtgctcatcg
     1861 atcatctggc gcacgttcat gcgataaatg tgatcggcgc caaacacggc cacgagctta
     1921 gggctgtgcc gctccagcaa atataagttt tgatagacgg aatccgcggt tccctgatac
     1981 caatcctctc cagaacgcat ttgcgccggc accggagtga cgaagtgttc gccgagaaag
     2041 tcgctggtgc gccaaccgcg atcgagatgc tcgagcaagg attgggcttt gaactgagtt
     2101 aagacataga tcgataagat cccggagttg acaaaatttg acaggacaaa gtcaataatt
     2161 cgataacggg cgccgaacgg cacggcgggt ttggcccggt gctgtgtcag cggatagagg
     2221 cgctcgccac gtccgcccgc gagtaccacg cctaagacgt acatcattgc ctcttttagg
     2281 ttccccctag ctcacgagtt gccggtacag ttcggtgtaa ttttttgctg agcgattcca
     2341 ggaaaaatcc attgccatcg ctcggcgctg caatgcgctc cacgccggcc ggtttcggta
     2401 aaccagcagg cattcgtcta gggcagcaag cagtgcgtcc gccgtatagg ggccgaatag
     2461 aaagccggtg cccgagcgcg cgctgacatt gcattgctgg acagtatcct tcaaaccgcc
     2521 gaccgcacgc accaccggta tggtaccata ctttaggctg aacatctgat tgagaccgca
     2581 gggctcataa agagagggca tcaggaaaat gtcggcaccg gcctcgattt gatgagccaa
     2641 aggattgtcg aagccgatac gcaccgcaac ttgctccgga aagcgtgcca tcgcagcgcg
     2701 aaagaagttt tcatagcgca cgtcgccgtt ggccagtagg atgagttgga cgccgcgcgc
     2761 catcagcgcg tccaggatgc cttcgaccag atcgacccct ttttgcggcg tcagtcgtga
     2821 gatcatgccg atgacggggc tgtcttcccg gctcggaagc ccgacgatct gcctgaggtt
     2881 ttctttgcaa gcgcttttct ggctgaggct gttttcaccg tagtgatccg tgagaaaagg
     2941 atctgtccag ggattccata actggtagtc cactccgttg agaataccga caagatcgtc
     3001 ggcgcgctgg cgcagcaccc cttccaaacc gaagccttgc tcaggggtta aaatttcggc
     3061 cgcgtaggac gggctcacgg tggtgagctt atcggcaaaa atgagcgcgc ccttgagata
     3121 gttaatgttg ccgtaaaatt ccaaatatgg cggcgtaaaa aagaccacgt ccagatccaa
     3181 caggcgccaa tcggcagggc cgaagatccc ctgaaagccg aggttgtgaa tcgtaaagac
     3241 ggtcttggcc cgtgcgattt cgctatatcg agccgcctgg gtcttgagat aaaccgtcat
     3301 caacgcggtt tgccagtcat gacaatggac gacgtccgca ggccgctggc ggagcagctc
     3361 caatgccgcg cggctaaaaa agacgaagcg ctcggaatta tccggataat cgcctgctgg
     3421 cgttgtgtaa agaaattccc gatcgaagta ctggtcggcg cgaataaaaa aaattctcac
     3481 ccggttcccg attaaggtct ccaaaacttc cgctaacacg ctgcgaccgc tgatcgggac
     3541 agaaaactgc atcgaggttt gttgcaatgc aaatccgccc cggaggacag aacggtatgc
     3601 gggcgcaatt acaaagactt cattaccaag ctgctccaat actacggaaa ggctcccgag
     3661 cacgtcggcc aaaccgccgg tcttagcgaa cggcgtaact tccgacgcga tcaacgcgat
     3721 tttcattggg ctcagc
//
LOCUS       DASULZ010000736         2252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_286588, whole genome shotgun sequence.
ACCESSION   DASULZ010000736 DASULZ010000000
VERSION     DASULZ010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286588"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..582)
                     /locus_tag="VFY96_15150"
     CDS             complement(292..582)
                     /locus_tag="VFY96_15150"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEX5607861.1"
                     /translation="MSIRLIVGINAALGKGSELAELYKARCAEISREPGCEQFEVFQS
                     VVNPDRLAILERWTDQAALDVHAKANAARAPLPPELRVGTTEREDYVYNRTR"
     gene            complement(739..1401)
                     /locus_tag="VFY96_15155"
     CDS             complement(739..1401)
                     /locus_tag="VFY96_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEX5607862.1"
                     /translation="MPGTQLKWRGRFVAWFSFALPLSLVLSIGATPIPAVVLFLGDSI
                     TAGYGLDISQAYPALIQQRIEAKNWPFKVVNAGQSGDTTAGGLNRIDWLLRSRIDVLV
                     LELGGNDGLRGVPAETIRKNLQAIIDRVKTKYPNAKIILAGMKLPPNMGRSYTQQFES
                     VFVSIAKKNNAPLIPFILEGVGGVSDLNLPDGIHPTVRGQQIVANNVWKVLEPVLRSL
                     LK"
     gene            1472..2179
                     /locus_tag="VFY96_15160"
     CDS             1472..2179
                     /locus_tag="VFY96_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5607863.1"
                     /translation="MNDTILSVEHLGKRFKSGQQIVDVLSDISFSVEKESTCAIIGPS
                     GSGKTTLIAICAGLERPSEGAVFLDGVALHRSSEEDLARLRNQSIGFIFQNFQLLPSL
                     TAVENVMVPAEIRGENHARSRASDLLKQVGLNGRFDHYPVQLSGGEQQRVAIARAFMN
                     QPKILFADEPTGNLDAETAKEIIKLLFELNARRGTTLLLVTHDRELTRQARRLIRLRG
                     GRLVLDSDEPKTKPAQA"
BASE COUNT          519 a          592 c          611 g          530 t
ORIGIN      
        1 gaagcaccgg tttgaggtga ttttttggta ggccgacaaa tccgtggctc caaatcattg
       61 ctgatggtca atggccgtag tatcgagttc cccgtcgctt gtaatgccct ggaacaggat
      121 tagtcaataa gaaaatacca aaacttccaa attgcgtgta taggcggtcg cgctgaccac
      181 aggatgtgaa aaaaaggaaa ttttaagtca aacctagaag cctgcgaacc gttccccgct
      241 tcgccaatgc gttcaagcgg acttgatcaa gtggacgttc agcgttggga gctaacgagt
      301 acggttgtaa acgtaatctt cgcgctccgt tgttcccact cgtaattcag gaggcaatgg
      361 cgcacgcgcg gcgtttgcct tggcatgtac atccaaagcc gcctgatcgg tccatcgttc
      421 caaaatggca agcctgtcgg gattcacgac actctgaaaa acttcaaatt gctcgcagcc
      481 cggctcgcgg gaaatttcgg cgcatcgagc cttgtatagt tccgccagct ccgaaccttt
      541 tcctagcgcc gcattgattc ccacgatcag tcgaatcgac attggtttcc tccgtttcgg
      601 ccagcggcct atctaatgtg acgactatca ttgtcttcat gcggcgctgt caagtacgaa
      661 agctggcgtt cgtcagcgtt gagaattttt cgtttcgcga gcggcgattt cgcggtgcag
      721 acggtttctg caagaccgct atttcagcag agatcgcaag acaggttcga gcactttcca
      781 tacattgttc gcgacaatct gctggccacg gacggtcggg tgaatgccgt cgggcagatt
      841 gagatcgctt acaccgccga cgccctcgag aataaaagga atcagcggcg cgttgttttt
      901 cttggctatg gaaacaaaaa ccgactcgaa ctgttgggtg tagctacgtc ccatattcgg
      961 tggcagcttc atgccagcaa ggatgatttt cgcgttgggg tacttggtct tgacccgatc
     1021 gatgatggcc tgaagatttt tccgaatggt ttcggcagga acgccccgca agccatcgtt
     1081 gccaccgagc tcaagaacca aaacgtcgat acgactgcgc agaagccaat caattcgatt
     1141 gaggccccct gccgtggtgt cgccactctg tccggcattg acaactttga atggccagtt
     1201 tttggcctcg atcctctgtt gaatcaaggc gggataggcc tggctgatgt cgagaccgta
     1261 accggctgtg atgctgtcgc cgagaaacaa tacgacggcc gggatcggag tagctccgat
     1321 ggataaaacg agcgagagag gcagcgcgaa tgagaaccaa gcgacaaacc tgccgcgcca
     1381 cttgagttgt gtccctggca taaaaattct acagttgatt ggctttgcgg tacactgccg
     1441 gaattcgcga ccttcacccc taaacacttt catgaatgac acgattttaa gcgtagagca
     1501 tttgggcaag cggtttaaga gcggtcagca gatcgtcgat gttctcagcg atatttcctt
     1561 ctctgttgaa aaggaaagta cgtgcgcaat catcggtccg tctggtagtg gcaagaccac
     1621 actgatcgcc atttgtgccg gcctggaacg accgagcgaa ggagcggtgt ttttagatgg
     1681 tgtcgcgctg catcgctcga gcgaggaaga ccttgcccgg ttgcgtaacc aatcgatcgg
     1741 gtttattttt cagaattttc aattgctgcc atctctgact gccgtggaaa atgtcatggt
     1801 tccggcagag attcgcggcg aaaaccacgc acggagccgc gcctcagatc tgctcaaaca
     1861 agtcggtctc aatggtcgtt tcgatcatta tcccgttcaa ctttccggcg gcgagcagca
     1921 gcgcgttgcg atcgcccgag cgttcatgaa tcagccaaaa attctctttg ccgatgagcc
     1981 tacgggcaat cttgatgcgg aaaccgccaa agagatcatc aaactgctgt tcgagctcaa
     2041 tgcgcggcga ggaacaacgc tgcttttggt gacccacgat cgcgagctca cccgccaggc
     2101 gcgccgctta atccgtttgc gcggcggcag gctggtgttg gacagcgacg agccgaaaac
     2161 caaacccgct caggcatagc tttcggacga tgaaacttcc tttgcaatcg tgggtctggc
     2221 aactcgcgcg tcgagatcta cggcagggcg tg
//
LOCUS       DASULZ010000737         3780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1097351, whole genome shotgun sequence.
ACCESSION   DASULZ010000737 DASULZ010000000
VERSION     DASULZ010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097351"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="VFY96_15165"
     CDS             complement(<1..845)
                     /locus_tag="VFY96_15165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5607864.1"
                     /translation="MKWNLMLAKLKFQRKKLIGLLAVAGMAAGGWYWYSAAAQKPRRE
                     NLVPITVTRGTIEEVVTAQGKLEPKQYVDVGTQVSGQLKKIHVDIGNAVHKGELLAEI
                     DPRVYQAQVEAGEAKLNSLRAQLNQQKAEAVLAEQNLKRNQNLIAVNAVSQQALQETE
                     SQAAVAKAQVDSISAQIQETQSNLKASQTNLGFTKIYAPMDGTVTTLPTKEGQTLNAN
                     QTTPTVMQVANLDTMTVRAQVAEADVNRLKETMPAYFTTLGDSEHRWQGTVRQIQPSP
                     EIVNDV"
     gene            complement(977..1405)
                     /locus_tag="VFY96_15170"
     CDS             complement(977..1405)
                     /locus_tag="VFY96_15170"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEX5607865.1"
                     /translation="MSKTAKLVFLASLILNIIFLGFVLGHVPRSFSGTPSRQERMEQG
                     LKKLPEPAQSHIRAVFARIRTAGDPVRDKMDASRGEALRVLGAERFDETAYDREVAKF
                     DQLREQMFKQIGQAIKQSAKELSPDERRMFADLLRRPANR"
     gene            complement(1402..1713)
                     /locus_tag="VFY96_15175"
     CDS             complement(1402..1713)
                     /locus_tag="VFY96_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607866.1"
                     /translation="MKDERDDKLDLWLRQRQVTPATSDLAQRIILRSQQVPQIKAISL
                     WQSIRALCAEFHLPKPAYVLASALTIGLVIGFSTPQDTDSSGNDVVAAQSFLAVDEAM
                     L"
     gene            complement(1727..2305)
                     /locus_tag="VFY96_15180"
     CDS             complement(1727..2305)
                     /locus_tag="VFY96_15180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5607867.1"
                     /translation="MSDLVTQSMPTAWPEKDDNQLLSLIQSGSSQAFSTLVERHTERF
                     YRLAYRYLQSREAAEDVVQDAFIKLWENPFIWQPEKNSKFTTWFYRIVVNLCLDFRKR
                     KTPAPLDDGTQLIDEREPMDETMIRAQEQKMLEKEIAALPERQRTALNLCFDEGLTNQ
                     EAAEIMGLNLKALQSLIMRAKTTLKGRLKSIL"
     gene            complement(2426..3025)
                     /locus_tag="VFY96_15185"
     CDS             complement(2426..3025)
                     /locus_tag="VFY96_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607868.1"
                     /translation="MNILNDNLHSAHCRVCKQRVGEMLKAIYGTCHQNYSFGWSASPH
                     DYAKTPIGTTLERIQSSLENFRGHRDFIKSPLVPPCDYYVSAPPFILEFDENQHFSRA
                     RLIALSLYPEHINLGFSIGYWCELCRSLDARDDEPFDRDERRAWYDTLRDLVPTLYGF
                     APTARLYGGAYLWCSLDSASIRDRERFLGWISSRLAGNP"
     gene            3161..>3780
                     /locus_tag="VFY96_15190"
     CDS             3161..>3780
                     /locus_tag="VFY96_15190"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5607869.1"
                     /translation="MAKPASIIGAPVGRVEGAEKVSGQAIYGADVHFPNTLWGKILRS
                     PYSHARIKKIDTSKAWQVDGVKAIVTGKDEPNHYQGKSIRDIPVLCWDKVRYVGDKVA
                     AVAAESREAAEEALQLIEVKYEELPAVFDALEAMNADAPILHEDPAAYDGAPQDILVK
                     DIPNVLNKLSWGKGDVEQGFREADLVLEHTFRIPIHHQGYLEPQSFL"
BASE COUNT          831 a          980 c         1069 g          900 t
ORIGIN      
        1 acgtcattga cgatttcggg cgagggctga atctgccgaa ccgtgccttg ccagcggtgc
       61 tcgctgtcgc cgagggtagt gaaataggca ggcatggttt ccttcaagcg attgacgtcg
      121 gcttcggcga cttgcgcgcg cactgtcatg gtgtcgagat tggcgacctg catgaccgtc
      181 ggcgtagtct gattggcgtt caaggtttgg ccttctttgg tcggcagggt ggttactgtg
      241 ccgtccatcg gcgcatagat cttggtaaac cctaagttag tctggcttgc tttcagattg
      301 gattgggtct cttgaatctg agcgctgatg gaatcgacct gcgctttggc gaccgccgcc
      361 tgggattccg tttcctgcag cgcctgctgg ctgactgcgt tcacggcaat cagattttga
      421 ttacgcttga gattctgctc ggcgagaacg gcttcggcct tctgctgatt gagttgggcg
      481 cgcaagctat tgagcttggc ttcgccggct tccacttgcg cctggtaaac acgcggatcg
      541 atttccgcga ggagctcgcc cttatgcacg gcgttgccga tgtcgacgtg gatttttttt
      601 agctggcctg aaacctgtgt gccgacatcg acatattgtt tgggttccag cttgccctgg
      661 gcggtgacca cttcctcgat tgtgcctctg gtaaccgtga tcggcaccag attttcccga
      721 cgcggctttt gcgcggcggc gctgtaccag taccaaccgc ctgcggccat cccggccacg
      781 gctaaaaggc cgatcagctt ttttcgttga aacttgagtt ttgcaagcat taggttccac
      841 ttcatctcgg ttacgcctcc caccaactgt catatcttaa tacgacctgg cggcgcttag
      901 tcttcgttcg acgatttcaa aaaaaagcac cggaaatacc ggtgcttagg aggtaaagct
      961 gcttgactca gatgggttag cggttggccg gacggcgcag gaggtcggca aacatgcgcc
     1021 gttcatcggg cgagagctct ttcgcggatt gcttgatcgc ctggccaatt tgcttgaaca
     1081 tttgttcacg caattggtcg aatttggcga cttctcggtc ataggccgtt tcgtcgaagc
     1141 gctcggcacc tagcacccga agagcctcgc cgcgagatgc atccatttta tcccgaactg
     1201 ggtcaccggc agtacggatc cgagcaaaga ctgcccggat atgcgattga gctggctcgg
     1261 ggagcttctt cagcccctgt tccatccgtt cctgacgcga gggagttccg gaaaaacttc
     1321 gtggcacatg acccaaaacg aagcctagga agatgatgtt taaaatcagc gaggcaagaa
     1381 atacaagttt cgccgttttg ctcatagcat cgcctcatct actgccaaga agctttgtgc
     1441 tgcgacaacg tcgttgccgc tgctatcggt atcctgtggg gtgctgaaac cgatcaccag
     1501 accgatcgtt agcgcgctgg ccagaacata ggcgggtttc ggcaggtgga attcagcgca
     1561 aagcgcgcga atcgattgcc ataacgaaat ggcttttatc tggggtacct gctgtgaccg
     1621 caaaataatt ctttgcgcca gatcggaagt cgctggggtc acctgccgct gccgtaacca
     1681 gagatccagt ttatcgtcgc gctcgtcttt catagacata ggtacctcat agaatacttt
     1741 tcaggcgccc tttcaacgtt gttttagcgc gcatgatcaa agattggagc gctttgagat
     1801 taagtcccat gatttcggca gcctcctgat tcgtcaaccc ttcgtcgaaa cagagattca
     1861 aagccgttcg ttggcgttca ggcagcgccg caatctcttt ttcaagcatt ttctgctcct
     1921 gagcccggat catcgtttcg tccatcggtt cgcgttcgtc gattagctgc gtcccatcgt
     1981 ccagcggtgc gggcgtcttc cgtttgcgaa aatcgagaca taagttcaca acgatgcgat
     2041 aaaaccaagt ggtgaatttg ctgtttttct ccggttgcca aatgaatggg ttctcccaca
     2101 gcttgataaa cgcgtcctgc accacgtcct cggcggcttc gcggctttgt agataacggt
     2161 aggcgaggcg gtaaaagcgt tcggtgtggc gctcgaccag tgtcgaaaag gcttggctgc
     2221 tgccgctctg gatcaatgaa agaagctggt tgtcgtcttt ctccggccaa gctgtcggca
     2281 tcgattgggt tacaaggtct gacaatgtgc tttccgagct tgatcatact acctgccggc
     2341 tccaaaaata aggctgaata caaaccgttc aatgagtgat acgagtggag gagaaaaagt
     2401 cttcgatatc gcaaatgctg gtacttcagg gatttccggc caatcggctc gaaatccatc
     2461 caagaaaacg ctccctgtcg cgaatggaag cgctatccag cgagcaccat aagtaggcgc
     2521 cgccataaag ccgggcagtg ggagcgaaac cgtacagggt cggaaccaaa tcgcggaggg
     2581 tatcatacca ggcgcgccgt tcgtcacggt cgaagggctc atcgtcgcga gcatccagac
     2641 tgcggcatag ttcgcaccaa tagccgatcg aaaacccaag gttgatgtgt tcgggataga
     2701 gcgaaagcgc gatcagacgc gcacgggaga agtgttggtt ctcgtcgaat tctaagataa
     2761 aaggcggagc tgaaacgtag taatcgcagg gcggaaccag cgggctcttg atgaaatcgc
     2821 gatgaccgcg gaaattttcc aggcttgact gaatccgttc cagtgtcgtg ccgatgggcg
     2881 tcttggcata gtcgtgcggc gacgccgacc agccaaagga atagttctgg tggcaggtgc
     2941 cgtagattgc tttaagcatt tcgccgaccc gctgcttaca cacccggcag tgggcggaat
     3001 gtagattatc gttcaaaatg ttcaactgct tttgctccgt tcaaccgctc cgctcagttc
     3061 aagctgggaa atggaaatag acttgcacga ttgaaccgct ggaacgattg aactttattc
     3121 atagaagatt aacacagagt tgacgaagga gaatgttgta atggcgaaac cagcatccat
     3181 catcggggct ccggttgggc gcgttgaggg cgccgagaaa gtcagcggtc aggcgatcta
     3241 cggcgccgac gttcacttcc cgaatacctt gtggggcaag atcctacgca gcccctattc
     3301 ccatgcgcgg attaaaaaaa ttgatacctc gaaagcgtgg caggtcgatg gcgtcaaagc
     3361 gatcgtcacc gggaaggatg agccgaatca ctatcaggga aaaagcatcc gcgacattcc
     3421 tgttttatgc tgggacaagg tgcgttacgt gggcgacaag gtggcggcgg tcgccgccga
     3481 gagccgcgaa gctgccgagg aagcgctgca attgattgaa gtcaaatatg aagagctgcc
     3541 ggcagtattc gatgcgctcg aggcaatgaa cgccgacgcg ccgattttgc acgaggatcc
     3601 agccgcgtac gatggcgccc cgcaggatat tctcgtcaaa gacataccca acgtgctcaa
     3661 taagctcagc tggggaaaag gtgacgtcga acaggggttt cgcgaagcgg atctggtcct
     3721 ggagcacacg tttcgaattc caattcatca tcaaggttat ctcgaaccac agtcgtttct
//
LOCUS       DASULZ010000738         4782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1097481, whole genome shotgun sequence.
ACCESSION   DASULZ010000738 DASULZ010000000
VERSION     DASULZ010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097481"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VFY96_15195"
     CDS             complement(<1..843)
                     /locus_tag="VFY96_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX5607870.1"
                     /translation="MPADRAKRAATYLWWQRGIIYQVYPRSFMDSNGDGVGDLRGLVS
                     RLDYLRGLGIDAVWLSPIYPSPMKDFGYDVADYTGIHPLFGTLEDFDLLIREAHAREI
                     KLVLDFVPNHTSDQHPWFIESRSSRGNPKRDWYIWRDPAPSGGPPNNWLSCFGGSAWE
                     YDRETEQYYYHAFLREQPDLNWRNPQVIEAMLSVLRFWLQRGVDGFRVDVLWHLIKDE
                     QFRDNPVNRGWHEGADPYQKLLALHSTDQAEVHAVVTRMRRLVDQYQDRVLIGEIYLP
                     VERLV"
     gene            complement(867..2741)
                     /gene="treZ"
                     /locus_tag="VFY96_15200"
                     /pseudo
     CDS             complement(867..2741)
                     /gene="treZ"
                     /locus_tag="VFY96_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009107058.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
     gene            2953..4632
                     /locus_tag="VFY96_15205"
     CDS             2953..4632
                     /locus_tag="VFY96_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HEX5607871.1"
                     /translation="MINDLWYKNAVIYCLSVATYMDANGDGIGDFQGLMRRLDYLHGL
                     GITAIWLMPFQPSPYRDDGYDVADYYGVGSRHGTLGDFVEFTHAAAQRGIRVIMDLVV
                     NHTSDRHSWFQEARKNPNSEYRDWYVWSDKKPPDANKGMVFPGVQKSTWSYDKIARSW
                     YFHRFYNFQPDLNTSNPYVQAEILKIMGFWIQLGVVGFRMDAVPFIISTKGARAKTVE
                     QYDMLRAFREFLQWRKGDAIILAEANVLPETDMEYFGADGDRMHMMFNFQVNQNLFYA
                     MAAADSRPLIKALQATKPRPATAQWGLFLRNHDELDLGRLTDEQRQRVFNCFGAQKEM
                     QLYNRGIRRRLAPMLNGDRRRIELAYSLMFTLPGTPVIRYGDEIGMGDDLNLSERNCA
                     RTPMQWSTEPHAGFTKSDRPILPVISDGPYGYEHVNVAEQRRDPNSLLNWTERIIRMR
                     KEVPEVGWGDFQVLPIRDPAIFAIRYDWRNNSVLFVHNLDSIPREIAFSTGLSGDQGR
                     LLVNLLTEDHSQAADNGKHHLLLEAYGYRWYRVGGLDYLLKRSEIETAKVR"
BASE COUNT         1085 a         1310 c         1329 g         1058 t
ORIGIN      
        1 caccagccgc tcgaccggca ggtagatctc cccaatcagc acgcgatcct gatactgatc
       61 caccagacgg cgcatgcgtg tcacgacggc gtgcacctcg gcctgatccg tgctgtgcag
      121 cgccaataat ttttggtagg gatcggcgcc ctcgtgccaa ccccgattaa cggggttgtc
      181 acgaaattgc tcgtctttga tcaaatgcca gaggacatcg acgcgaaaac catcgacgcc
      241 gcgctgcaac caaaagcgca gcacgctgag catcgcctcg atcacttgag ggttgcgcca
      301 gttcagatca ggttgctcgc gtagaaacgc gtgatagtag tattgctccg tttcccgatc
      361 gtactcccat gcgctgccgc caaagcatga gagccagtta ttcggcggcc cgccgctcgg
      421 cgccggatcg cgccagatat accaatcacg tttggggtta ccgcgcgatg agcgcgattc
      481 gatgaaccac ggatgttggt cggaagtatg gttaggaaca aaatccagaa ccagcttgat
      541 ctcgcgcgcg tgcgcctccc ggatcaaaag atcgaaatct tccaaagtgc cgaatagcgg
      601 atgaatcccg gtgtaatccg ccacatcata tccgaagtcc ttcatcggcg aaggatagat
      661 cggcgatagc caaacggcat cgatgcccag tccgcgtaga taatcgagcc gtgagaccag
      721 gccgcggaga tcgcccacgc catcgccgtt gctgtccatg aaagagcggg gataaacctg
      781 gtaaatgatg ccgcgctgcc accacagata cgtcgcggct ctcttggcgc ggtcagcagg
      841 catcggagtg gcttcagctg tcattgtcag tgatcggatt tgatttttcg cgctctgggg
      901 aaaagcacca ccgcggcctg tccagggatg cgccagcctt cttctgtctc cagggcggcc
      961 gttccagcac ctccgtatct aggattctcg ctggaccata gaatgtccca gcgttgtcct
     1021 tctggcggcg cgagcagcgg ttcgggagct atggaaagat gcaaatcggc tcctaaattc
     1081 acgaccacaa gccggtcgcc ttgttgattg ccgaaccagc gaatcaggaa tgcttcagag
     1141 cccaggatcg cgccgtcgaa tgatccagcg cgcggtttac tgaacagggg atcggtcttg
     1201 cgcaggcgaa ttagatcgcg atgcagcgtg taggcttcag aatgagcttc gcgctcgttt
     1261 agatcgagct tgcatctctc gaatgtagcg cgatctgccg gattgggaag gaggcgttgc
     1321 atttccggcg ttgccaggct gcggaactgc gccaaaaact cggcgcggcc tttgcgcact
     1381 tcctgggcaa gctctggcgg gtgatcggcg aaaaagagaa acggacggga tgacgcaaat
     1441 tcttggcctt gaaacagcat cggagttgcc ggacccagca gcagcaatgc cgtgatcgcg
     1501 cgatagcgcc cgggactggt cagtcgatcg aggcgaagcc cgcaaccgga gtttgcgacc
     1561 tgatcatggt tctgaatgta agtgatgaac ttagccggtg aaacgccaaa ggcaggtgtg
     1621 ccgcgtcgct ttttttgcca gctatagcgc tggccctgat agagatagcc ccattttagc
     1681 gcagagacga gctcctgagg agtgccgtgg taatccgtgt agtaggcgtc ctttcgtccg
     1741 gtcacggcga caacggcgct atggtggtag tcatcgttcc ataaaccatc catgccataa
     1801 ccgccttgct cgacgggacg gatgagcttt acatgctgcg gctcattctc cgcgatgatg
     1861 acaatggctc gtcccggcgc tgcccttcgc gcgaaacgcg taatctctgc cagaatatgt
     1921 ggtgaggtcg tatcgaaaat ctgctgggtt gcgtcgagac gaagaccgtc gagatgaaac
     1981 tcggcgatcc agtagccggc gttggcggtg atgaactcgc gcaccggcgc ggagttcttg
     2041 ccgtcgaaat tgatcgccat gccccattcg ttttcataac ggtcggtgaa gtagtcgtcg
     2101 gaaaattctt tgaagtaggc gccgtccgga ccaaaatggt tgtagaccac gtcgagcacc
     2161 acggcgagtc caatcccatg agcgcgatcg acaaaacggc gaaaatcgtc cggcgtccca
     2221 tataatcgag tcggcgcata cagatcgacg ccgtcgtatc cccaaccgaa ggcaccgctg
     2281 aaatcggcca ccggcatgac ctccagcagg gtcactccca attgggccag actttccaac
     2341 tctacagtgg ccgcgttcca ggttccttca cgggtaaaag tcccgatatg catttcgtaa
     2401 acaatttggc ctgcgagccc aatgccgcgc caatttccgt ccgtccagcc aaacccgtcc
     2461 gaatcgatga tctgcgaggg accgtgcggc ccgtcaggtt gaaaacggga ggcgggatca
     2521 ggataggaat ttttagcatc caaccggtat cgatagagca tgtccggagc tgcattgagt
     2581 atcactcctg agaaatagcc attgccctcg cgatcaagcg cataaggcgc cacatcatcg
     2641 cctctgccca gcattacctc tactgttctt cgttttggcg cccagacgcg aaagtggact
     2701 ccgcccgacg gctgaggttc cgcgccgacg ggtaggcgcc ggactaactc tggcgtattt
     2761 tcaaaagcaa actgtccagt agtttcggaa gagaaatttc ccgcgccgct gtccatctaa
     2821 gtaccataag ttcttaaagc gctggcctca agataatgac gaggtatttt ctattacttc
     2881 tcgaaaaagc gcatcgaagt acattccatt cgaccagatt tttactattc tgaaataagg
     2941 gggaattcag ctgtgatcaa cgacctttgg tacaagaacg cggttattta ttgtctgtca
     3001 gtcgcgacat atatggatgc caatggggat ggcatcggtg attttcaggg actcatgcgg
     3061 cgtctcgatt atctgcatgg gctcggaata acggccatat ggctaatgcc gttccaaccc
     3121 tccccgtatc gcgatgacgg atacgacgtg gccgattact acggggtggg ttcgcgtcac
     3181 ggcacactcg gcgatttcgt cgaattcacg catgcggcgg cgcaacgtgg cattcgcgtg
     3241 atcatggatc tcgtggttaa tcatacttct gaccgccatt cttggtttca ggaggcgcgc
     3301 aagaatccaa attccgaata ccgggattgg tatgtttggt cggacaagaa accgcccgat
     3361 gcgaacaagg gaatggtatt tccgggagtt caaaaatcga cgtggtccta cgataaaatc
     3421 gcccgttcct ggtattttca tcggttttat aattttcaac ctgatctcaa tacctcgaat
     3481 ccctacgtgc aggccgaaat cctaaagatc atgggattct ggattcaact cggggtggtc
     3541 ggatttcgca tggatgcagt accttttatc atctcgacaa aaggcgcgcg ggccaaaacc
     3601 gtggagcaat acgacatgct ccgggcgttt cgtgaattcc tccaatggcg taaaggcgat
     3661 gcgatcattc ttgctgaagc caacgttttg cccgaaaccg atatggaata cttcggcgca
     3721 gacggcgacc gcatgcacat gatgttcaac tttcaggtta atcagaattt attttatgct
     3781 atggcggctg ccgattctcg gccgctgatc aaagccctgc aagcgacaaa gccccgccca
     3841 gcgaccgcgc agtggggcct attcctgcgt aatcacgacg agcttgatct gggccgattg
     3901 actgacgagc aacgacaacg cgtgttcaat tgttttggtg cccaaaagga gatgcagctc
     3961 tataaccgtg ggattcgccg ccggctcgcg cccatgctca acggcgaccg caggcgcatt
     4021 gagcttgctt atagtttgat gttcacgctc cccggcacgc cggtgattcg gtatggagac
     4081 gagattggca tgggggatga tctcaatctg tcggagcgca attgcgcccg caccccgatg
     4141 caatggtcaa cagagccaca cgcgggattc acgaaatcgg atcgaccgat attaccggtg
     4201 attagcgacg gcccatacgg ctacgagcat gttaacgtcg cagaacaacg gcgcgatcca
     4261 aattcgctcc tcaactggac agaacgaatc atccgtatgc gtaaggaagt tccggaggtt
     4321 ggctggggcg atttccaagt gcttccgatt cgcgacccgg cgatttttgc aattcgctat
     4381 gactggcgca acaattccgt cctgttcgtc cataatctgg atagtattcc gcgggagatc
     4441 gcgtttagca cgggtttgtc tggcgaccaa ggccgattgc tggtcaatct cctgaccgag
     4501 gaccacagcc aggcggccga taatggaaag caccatctac tgctcgaagc atacggttat
     4561 cgctggtacc gagtcggcgg tctcgactac ttgctcaagc gcagtgagat cgaaacggca
     4621 aaagtccgct gatcgagagc catgcgagcg ggtattccgc tttgccgcct attgggacac
     4681 tgggagtcga tttcaggagg ccgcgatctt cggtcttcgt actgaaagtg aatgcagaca
     4741 gcttcgtaga aaaaatcata ttgcagtgaa tgtttccgcc at
//
LOCUS       DASULZ010000739         2233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1404645, whole genome shotgun sequence.
ACCESSION   DASULZ010000739 DASULZ010000000
VERSION     DASULZ010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404645"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="VFY96_15210"
     CDS             <1..1048
                     /locus_tag="VFY96_15210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5607872.1"
                     /translation="LYSMVEAAGSVSVGLLLWYGGGEVLHGIIGIGTLVAFNEYIHRF
                     FVPLRDFSQKYTVMQSAMASAERIFQLLDSPVDIANPRAPQVPKPFHGEVVFDRVWFH
                     YKPGEPVLKNVSFRIEPGEKVAVVGATGSGKTTTIKLLNRLYDIQKGSIKVSGVDVRD
                     WDLRALRRHIGVVLQDVFLFSGDVRANLALGNPSIPLEQIREAAEIANADRFIRRLPD
                     GYAAHVRERGSNFSGGQRQLLSLARVLVYQPEILVMDEATSSIDTETEALIQDALEKV
                     MRDRTCLLIAHRLSTIRNADRIIVLHHGEVREVGSHAELMDQQGIYYRLYQLQYEREE
                     LKLGAHDLPAKLLA"
     gene            1187..2194
                     /locus_tag="VFY96_15215"
     CDS             1187..2194
                     /locus_tag="VFY96_15215"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX5607873.1"
                     /translation="MLRAAYEEGKILWRREPITDFVDSAGVQADTREKLKLVLAVRKY
                     ARDVLKFNVGGSYSSYSRVDRPDLTYIVMAAPETELRPYTWWFFIVGNVPYKGFFSRD
                     EAEAEIARLKAQGYDTTLRTSAAFSTLGWFDDPLLSHLLRYDKAILTEIVFHELFHNT
                     LYIKGAGAFNESSANFVGHRAAIDFFRIRFGAASQEYARAVQIWNDEREFGHFIDEVA
                     HTLRDLYSRKIPREAKIRLREQVFARSKEEWQRRIADRPNHSFRGFGQRPLNNAVLMH
                     YLVYLKGLDLFESLYDACGQDLGRTIETLRKAVDKGGEPFEAVRTWLDKNRQQTAAIA
                     D"
BASE COUNT          463 a          593 c          668 g          509 t
ORIGIN      
        1 gctctattca atggtcgagg cggcgggttc cgtcagcgtc ggtctactgc tttggtacgg
       61 cgggggcgag gtgctgcatg gcatcatcgg cattggcacg ctggtcgcgt tcaacgaata
      121 tattcatcgg tttttcgttc cgcttcgcga tttcagtcag aaatacacgg tcatgcaatc
      181 cgcgatggcg tcagcggaaa gaatctttca gctgctcgac agcccggtcg atatcgccaa
      241 tccgcgagcg ccgcaagtgc cgaaaccgtt ccatggcgag gtggtgttcg atcgggtttg
      301 gtttcactac aagcccggag agccggtact gaagaatgtc tcgtttcgta tcgagcccgg
      361 tgagaaagtc gccgtggtgg gcgccacggg ctcgggcaag acgacaacga tcaaattgct
      421 caatcgtctt tatgatatcc agaagggttc gatcaaagtc agcggtgtcg acgtcaggga
      481 ttgggatttg cgggcgctgc gacggcacat cggcgtggtg ctgcaggatg tttttctttt
      541 ttccggcgat gtgcgcgcca atctcgctct gggaaatccg tcgattccgc tggaacagat
      601 tcgggaagca gcggaaatcg ctaacgccga tcgttttatt cgccgcctgc cggacggcta
      661 tgcggcacat gtgcgcgagc gcggcagcaa cttttccggc gggcagcgcc agcttctgtc
      721 cctggcgcgc gtgctggttt atcaaccgga aattctggtg atggatgagg cgacctcaag
      781 tatcgacacg gaaaccgagg cgttgattca ggatgcgctc gaaaaagtca tgcgcgatcg
      841 gacttgtctg ctgattgctc accggctctc gacgatccgc aatgccgacc gaatcatcgt
      901 gttgcaccac ggcgaagtcc gcgaggtggg tagccacgcc gagttgatgg atcagcaggg
      961 catctattat cgtctgtatc agttgcaata tgagcgagag gagttgaagc tgggggctca
     1021 tgatctgccc gccaagcttc tggcgtgaat gtctgtgtgt ccgaacggtt ttagtcgtcg
     1081 ttgccgagtc cgttctttcc tgcggcgaag atttaaacaa cttttctggc tcactgcggt
     1141 aaccggcgtt attgccgcgg ttctcccagg ctgttcgcct ttatacgtgt tgcgggcggc
     1201 ttacgaggag ggaaaaattc tctggcgccg cgagccgatc accgacttcg tcgatagcgc
     1261 cggcgtgcag gcggacaccc gggaaaagct caagctcgta ctagccgtgc gcaaatacgc
     1321 acgcgatgtg ctgaaattca atgtgggcgg cagctattcg agctactcgc gggtagaccg
     1381 gcctgatttg acctatatcg ttatggcggc gccggaaacg gagctgcggc cttatacctg
     1441 gtggtttttc atcgtcggaa atgtgccgta caaaggcttt ttttcccgcg atgaggcgga
     1501 agccgaaatc gctcgattga aagcccaagg ctacgacacg acactgcgaa cttcggctgc
     1561 gttcagcact ctgggatggt tcgacgaccc gctgttatcg cacttgctgc gctacgacaa
     1621 agcaatctta accgagattg ttttccatga gctgtttcat aacacactct acataaaagg
     1681 cgcgggcgcc tttaacgaaa gctcggctaa tttcgtcggc catcgcgcgg ccattgattt
     1741 ttttcgcatt cggttcggcg cggcgagcca ggaatatgcg cgcgcggtgc agatttggaa
     1801 cgacgagcgt gaatttggtc actttatcga cgaggtggca cacacgctgc gcgaccttta
     1861 cagccgtaag attccgcgcg aagccaagat ccgattgcgc gagcaggttt tcgcgcgcag
     1921 taaagaggaa tggcagcgcc gcatcgccga ccgtccgaac cacagctttc gcgggttcgg
     1981 ccagcggccg ctgaacaatg cggtgctcat gcactacttg gtctatctca aaggcctgga
     2041 cttgttcgaa tcgctttacg atgcctgtgg gcaggatctc gggcgcacca tcgaaactct
     2101 acgcaaggcg gtcgacaagg gcggagagcc cttcgaagcc gttcgcacat ggctcgataa
     2161 aaaccgacaa caaacggcgg cgattgcaga ctagcgtggt gtacaagaag ttcgttaaaa
     2221 actacgaacg atc
//
LOCUS       DASULZ010000740         2903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_693413, whole genome shotgun sequence.
ACCESSION   DASULZ010000740 DASULZ010000000
VERSION     DASULZ010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693413"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VFY96_15220"
     CDS             <1..631
                     /locus_tag="VFY96_15220"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5607874.1"
                     /translation="QLFFIRERYRPHGHRRSGSFKSALSVSLLLLAVVEFIGGSVFWS
                     CAAWTPTALRSAKLLTIQQTGWIMSVLSIANMIGSFCLGSLSDKLGRRLVIMASAFPA
                     ALASFAVFYWLNSVAAIAAGIFLFGVLKASVPALIIALAQETAPAGSAGTAAGVIMSL
                     HYTSGVLAPLIAAKLISATGDILLTMILVSSVPLVVYGSLIGVVGERSR"
     gene            complement(638..1480)
                     /locus_tag="VFY96_15225"
     CDS             complement(638..1480)
                     /locus_tag="VFY96_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004003443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5607875.1"
                     /translation="MRIIDLHCYPNTEPWIKSQGPYVEALAKYWNRAWRWKSEAEVVE
                     EFKRAGVEAMLVAFDIESITGAPPCTNEYIAEMRDRNPGVIRQAWAAVDPLKGGQAIE
                     EAQRAIERFKLFGFHFHPIMGHFSVDDRKLYPLWETINRLKVPVMIDVGTTGMGAGMP
                     GGLGAVIRHAHPAAIDQLAADFPDLHIVAAHPGWPWTDEMLAVVLHKGNVSWELSGWA
                     PKYFPDALKRDIKGRLKEKIMFGSDYPSIPYERIFTEWKELAYSDEVMEKVFHQNAER
                     ILGL"
     gene            1669..>2903
                     /locus_tag="VFY96_15230"
     CDS             1669..>2903
                     /locus_tag="VFY96_15230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX5607876.1"
                     /translation="MFTQSWSTSERRYNVIVHRDVRVRMPDGTQLDGDIFRPDSPEKF
                     PVILGAHAYNKHLQSPPMLPVGFTPMRGYMESGDSTFFARRGYVHAVFNVRGSGSSEG
                     FYQLMGPQEVGDVSELIDWLAVQPWSSGAVGMFGVSYFARLAKAVAALGPSALKAIFA
                     PFAGTDDYRHRCYHGGMLAHGFISHWRNSLHRPNYRSLYKEKHGDAAYKEAIALALRD
                     EEIVAQPVLREALQNPDAGTNALIVDFVLHPFDGPFWREHSAQDEKATIPAYLGACWG
                     NYGLHLPGAFSAWRNWRGPKKMVIGPGVYLDRPVYQYQDEALRWFDFWLKGIETAVMD
                     EPPIRCFLPPTGEWKSLNDWPPPETRWTTFYLHKNRILSEHELWPAEGSDSFDESNFE
                     HGALTYSTPLLVENTEVMGP"
BASE COUNT          582 a          841 c          798 g          682 t
ORIGIN      
        1 acagctcttc ttcatccgcg agcgctaccg gccgcacgga cacagacgat cgggttcgtt
       61 caagtctgcg ctttccgttt cgcttttact cctcgccgtc gtcgaattca ttggcggctc
      121 ggtcttttgg agttgcgcgg cgtggacacc gacggcactg cgatcggcga aattactcac
      181 gatccaacaa accggttgga tcatgagcgt cctgagcatc gccaatatga tcggatcatt
      241 ctgtcttggc agcttgtccg acaagctcgg caggcgcttg gtgattatgg cgagcgcttt
      301 ccccgcggca cttgcctcgt ttgcggtctt ttactggctc aattccgtcg ctgccatcgc
      361 cgcgggcatt tttttgttcg gtgttctcaa ggcttcggtc cccgctctga tcattgctct
      421 cgcgcaagaa accgcgcccg ctggtagcgc agggaccgcc gcgggcgtga tcatgtcgtt
      481 gcattacacg tcgggagttt tggcgccttt gatcgccgcg aagctcatca gcgctacggg
      541 agacattcta ttgacgatga tcttggtttc gtccgtgccc ctggtggtct acggctcgtt
      601 gatcggcgtc gtgggcgagc ggagtcgcta atcggcccta aagccccaaa atgcgctcgg
      661 cgttctgatg aaaaaccttt tccatcactt cgtccgaata cgccaactct ttccattcgg
      721 tgaaaatccg ctcgtaggga atactcgggt aatcactgcc gaacatgatt ttctctttca
      781 gtcgaccttt gatgtctcgt ttcaacgcat cggggaaata tttcggcgcc catcccgaca
      841 attcccacga aacgttgccc ttgtgcagga cgacggcaag catctcgtcc gtccacggcc
      901 agcctggatg ggcggcaacg atgtgtagat ccggaaaatc ggccgcgagt tgatcgattg
      961 ccgcgggatg cgcatgacga atcaccgcgc ccaaaccgcc gggcatcccc gcgcccattc
     1021 ccgtggtgcc gacatcgatc atcaccggaa ccttgagtcg gttgatggtt tcccaaagcg
     1081 ggtacaactt gcggtcatcg acggaaaaat gacccataat cggatgaaaa tgaaatccaa
     1141 acagtttaaa cctttcgatc gctctctgcg cttcctcgat tgcctgtccg cccttgagcg
     1201 gatccacggc ggcccaggct tgccgaatca cgccaggatt tcgatcgcgc atctcggcga
     1261 tgtattcatt cgtgcacggc ggcgcgccgg taatcgattc gatgtcgaaa gccacgagca
     1321 tcgcctctac accggcccgt ttgaattcct ccaccacctc cgcttcgctc ttccatctcc
     1381 aggcccgatt ccagtatttt gccaatgctt caacatacgg tccctgcgat ttgatccagg
     1441 gctcggtatt gggatagcag tgaagatcga taattcgcac gcgctgaatc tcctctcgcg
     1501 tcgagcaata cgtcggctaa cttcaactgt caagaaatgc gaatgttggg ttataaatgt
     1561 catttcgacc cgggggagaa atccttccga gtcttgggac atattgcagc tactcgacat
     1621 tcagcgctgc ctcgagtaag cggaattcgg tcacggaggt taagaaatat gttcacacag
     1681 agttggtcga catcggaacg aagatacaat gttattgtac atcgcgacgt cagagtgcgc
     1741 atgccggacg gcacgcaatt ggacggtgac attttccgac ccgactcgcc agaaaaattt
     1801 cccgtgatcc tcggcgcaca cgcctacaac aagcacttgc agtcaccgcc catgctcccg
     1861 gtcggcttta caccgatgcg cggctacatg gaaagcggcg actcgacttt cttcgcgcgt
     1921 cgcggctatg ttcatgccgt ttttaacgtg cgcggcagtg gaagctccga aggcttttac
     1981 cagctgatgg gcccgcagga agtgggcgat gtcagtgagc ttatcgactg gctggccgtc
     2041 caaccctgga gcagcggcgc tgtcggcatg ttcggtgttt cttattttgc acgcctggcc
     2101 aaagccgtag ccgcgcttgg accgagcgcg ctaaaagcga ttttcgcgcc gttcgccggc
     2161 accgatgatt atcgccaccg ctgttatcat ggtggaatgt tagcccatgg ttttatcagt
     2221 cactggcgca acagcctgca tcggccgaat tatcgcagtc tgtacaaaga gaagcacggc
     2281 gatgcagcct ataaggaagc catcgcgctt gccttgcgcg atgaggaaat tgtcgcgcag
     2341 ccggtattgc gcgaggcttt gcagaatccc gacgccggaa ccaatgcgct gattgttgac
     2401 ttcgtcctgc atccgtttga tggaccgttc tggcgcgagc acagtgcgca ggacgaaaaa
     2461 gcgacgattc ccgcctacct tggcgcatgc tggggcaact acggtttgca ccttccgggt
     2521 gcgttttccg cgtggagaaa ttggcgtgga ccgaaaaaga tggtgatcgg acccggggtt
     2581 tatctcgacc gcccggttta tcaatatcag gacgaggcgc tgcgttggtt tgacttctgg
     2641 ctcaaaggca tcgagacggc cgtgatggac gagccgccta ttcgctgttt cctgcccccc
     2701 accggcgagt ggaagagctt gaatgattgg ccgccgccgg agacgcgctg gacaacattt
     2761 tacctgcaca aaaaccgtat attgagcgag cacgagctct ggcccgcgga gggctcggac
     2821 tctttcgatg aatcgaattt cgagcatggc gcgctcactt attcgacgcc gttgctcgtg
     2881 gagaacaccg aagtcatggg ccc
//
LOCUS       DASULZ010000741         2497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_287256, whole genome shotgun sequence.
ACCESSION   DASULZ010000741 DASULZ010000000
VERSION     DASULZ010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287256"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VFY96_15235"
     CDS             <1..341
                     /locus_tag="VFY96_15235"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5607877.1"
                     /translation="FLEIGATWLPYYLDRLDEHWEKRGEFEMPLLKKKPSEIVRDSKM
                     YFSVEPGESQLAATIDYVGADHFLYASDIPHWDNEFPHNLHDLQNHKQLSNETKEKIL
                     YKNAKELFAL"
     gene            686..1672
                     /locus_tag="VFY96_15240"
     CDS             686..1672
                     /locus_tag="VFY96_15240"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607878.1"
                     /translation="MSRISFTRVMFTTIFLGCTCISFAADTIRIAYSSINPHALLVSL
                     AEKRGLFAKYGLSSVVVYIPGGSTVVQAMVSGDVDLGQLTGAPGVAANLRGADILYIA
                     MTDDRMGYQLATRKDIKSPAELKGKRLGISRFASSADFGTRTLLKRLGIDPKEVTILQ
                     IGNEAERLTALKSGSIDGSVFNAPFGSVAKKFNFNILADAGALGIPYFNTGMCGSAKV
                     LQKNEGRIVNFLRAYLEAIKIFKTEPEYTLKALAQFSRVNDQELLKEAYDYNKNKIPD
                     VPYPSLKAMQAVVDPLVEADPKLGKVDARNYISDRYLKKLEDEGFVQKLMGR"
     gene            1742..2221
                     /locus_tag="VFY96_15245"
     CDS             1742..2221
                     /locus_tag="VFY96_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005370779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5607879.1"
                     /translation="MINSQEPIAIVAEEASERTKASSYPEPFASQMTGRRKQPLGDLF
                     GLTNFGVNLTRLTPGGVSALRHAHTRQDEFIYVLQGCPTLHTDEGRTRLSPGMCAGFK
                     AGTENGHRLMNETDEEVVYLEVGDRTPGDEGTYPDDDLKALMIDGKWKFVHKDDSPY"
     gene            complement(2309..>2497)
                     /locus_tag="VFY96_15250"
     CDS             complement(2309..>2497)
                     /locus_tag="VFY96_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX5607880.1"
                     /translation="LISGRGEALNENEEPVPGLYAAGDAIGGLMGGYRGGYTGGLMQA
                     VVTGILAGENAVPKITPD"
BASE COUNT          604 a          679 c          689 g          525 t
ORIGIN      
        1 catttctgga aattggcgcg acctggctac cgtactatct cgatcgtttg gacgagcact
       61 gggaaaagcg cggcgagttc gagatgccgc tgctcaagaa aaaaccgagc gagattgtgc
      121 gcgattcgaa gatgtatttc agcgtcgagc ccggggaatc gcagcttgcc gccaccatcg
      181 attatgtcgg cgccgatcac tttctctacg cttccgacat tccgcattgg gacaacgagt
      241 ttccgcacaa tcttcacgat ttgcaaaacc acaaacagtt atcgaacgag acaaaagaaa
      301 agatactgta caaaaatgcc aaggagctgt tcgcgttgta gactgaactg atacacggaa
      361 aatttcgaga atgtcgtgct gagcaccaga cggtgtgaaa ctcggctcat ggtttcgccc
      421 cacgagacgc gcaatgcgct actcggcacc tgatatcctg agtaggcagc gctacgctgc
      481 cgtatcgaag gatacagggt tcgcgctgat cgccaatcct gcaccggaag gccagcgtcg
      541 agcttcacag gtctgcggct agcgaagcag ctctttttca ggagttggag acgagatcct
      601 tcggtggagt ttacccaaag cgacgtcgga gggcctcaag gatgacagcg cgattgactt
      661 tggcagttcc acagaaggga acacgatgag ccgaatttct tttacgagag tgatgttcac
      721 gacgattttt ctcggatgca cttgcatatc atttgcggcc gatacgatcc gcatcgcgta
      781 cagctcgatc aatccgcatg cgcttctggt gtcactcgcg gagaagcgcg ggctgttcgc
      841 taaatacggg ctctcttcgg tggtggttta catacccggc ggtagcacag tggtgcaggc
      901 gatggtttcg ggtgatgtcg atctgggaca gctcaccggc gcgcccgggg tggcggccaa
      961 tttgcgcggc gccgatatcc tctacatcgc tatgaccgat gaccgtatgg gatatcagtt
     1021 ggcgacacga aaggatataa aaagcccggc cgaactcaag ggaaaacgcc tgggtatcag
     1081 ccgcttcgca tcatcggcgg atttcgggac acgcacgctt ctgaaacggc tgggcatcga
     1141 tcccaaagag gtcacgattc tgcagatcgg caacgaagcg gagcgtctga ccgcgctcaa
     1201 atccggcagc atcgatgggt cggtgtttaa tgcgccgttc ggtagtgtcg cgaagaagtt
     1261 caatttcaat attctcgccg acgccggcgc gctcgggatt ccttacttca acacggggat
     1321 gtgcggcagc gccaaagtgt tgcagaagaa tgaaggtaga attgtcaatt ttctgcgcgc
     1381 ctacctcgaa gcgatcaaga tcttcaaaac cgagccggag tacacactca aagcactggc
     1441 gcagttcagc cgggtcaacg atcaagagtt gctcaaggaa gcctacgact acaacaaaaa
     1501 taaaatcccc gacgttccat atccgtctct gaaagcgatg caggcggtcg tcgatccgct
     1561 cgtcgaagcc gatcccaagc tcggcaaggt cgacgcgcga aactatatca gcgatcgcta
     1621 tttaaagaag ctggaggacg aaggcttcgt gcagaaattg atggggcggt agtgtcctac
     1681 tcagaagttt ctgcaagctt caaacaccac actcgggcat actacacagg acaaaaatcc
     1741 gatgatcaat tcgcaggaac cgatcgctat tgttgcagag gaggcatcgg agagaaccaa
     1801 agcgtctagc tatcccgagc cctttgcgtc gcaaatgacc ggcaggcgaa agcagccgct
     1861 gggtgatcta ttcggcctta ccaacttcgg tgtaaatctg acccggctta ccccgggagg
     1921 agtttcggcg ttgcgccatg ctcacaccag gcaagacgag ttcatttatg tcttgcaggg
     1981 atgtccaacc ttgcacaccg acgaaggtcg aaccagactc tcgccgggca tgtgcgctgg
     2041 cttcaaagcc ggcactgaga acggtcaccg attgatgaac gagacagacg aagaggtcgt
     2101 ttatctcgaa gtcggagaca gaacgcccgg tgatgaaggc acctacccgg acgatgacct
     2161 aaaggcgctc atgatcgacg gcaagtggaa gtttgtgcac aaggacgact cgccgtactg
     2221 attcgcaaaa ttccaagcac cgaggcggga ccctctctga cctactcaca tgcttcgctt
     2281 agcttcacgt tgcgctgtcg aagcgttgtt aatcaggggt tatctttggc actgcgtttt
     2341 ccccggcgag aattcccgtt accaccgcct gcatcaagcc acccgtgtag ccgccgcgat
     2401 agccgcccat gagaccgcca atggcgtctc cagccgcgta aagacctgga accggttctt
     2461 cgttttcgtt taaggcctca ccccggccgc ttatcag
//
LOCUS       DASULZ010000742         5963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1097957, whole genome shotgun sequence.
ACCESSION   DASULZ010000742 DASULZ010000000
VERSION     DASULZ010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097957"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VFY96_15255"
     CDS             <1..281
                     /locus_tag="VFY96_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607881.1"
                     /translation="EEIYMTKQKFSRGLMTAVIVGAFGLGYVCGSMTQRQAQAQIGGL
                     LDKAGKAGGPVGSVAQFGSSLVEMQDHISGLQKNLDTFKQVQATLTGK"
     gene            497..757
                     /locus_tag="VFY96_15260"
     CDS             497..757
                     /locus_tag="VFY96_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607882.1"
                     /translation="MKLSIKAMMIAGALFKAVVFLFISLMNLILRPYGGAYLAFLSSV
                     YPGYDPINVPIALILGTIYSLIAGAFAGLLFGYLYNFFTERT"
     gene            complement(862..1800)
                     /locus_tag="VFY96_15265"
     CDS             complement(862..1800)
                     /locus_tag="VFY96_15265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX5607883.1"
                     /translation="MSRLKAARIHSYGPPEVISLEDLPLPEPGDGEVVVQVKAAGVGA
                     WDALIRRGRSALPQPLPLTLGSDLSGVIRSLGPGVRGFNVGDEVFGVTNERFTGAHAE
                     YAIAKARMIAHKPKTLNHTHAASVPVVAVTAWQMVFELAAVAPGQFVLVHGGAGNVGG
                     YLLQLAKRAGAMVVTTTAAKDTSYVRRLGAYGVIDYRVKRFEEQVKHVDVVLDTVGGA
                     TLERSYGVLKRGGILVSSAAEPSQEQAQQHGVRAMFFLVEVTTERLEKIAELIDAGEL
                     ATEVGEVLWLHEARQGHEMIEGAPHRRGKIVIKIAD"
     gene            complement(1928..2902)
                     /locus_tag="VFY96_15270"
     CDS             complement(1928..2902)
                     /locus_tag="VFY96_15270"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5607884.1"
                     /translation="MSTMTKIQFGVLLPHFGKEASPSRIIDGSKMCEELGFHSVWVRD
                     HLIWHPHGMETAGLKFVEPFVTLSAVAAVTKKIILGIAVLVPVRWPLKVSQDLASLSY
                     IAGGRVIAGMGLGFNPAELATVGLRIEDRIDILRETVEIARLIWKENSASYKGKIFSF
                     DNVTIEPKPVEPIPIFCGGSTRAAIRRAAEYCDGWIPGRVPMATFDDRIKYLRQLTDR
                     KITLGNIPITRIDKDRKKARASINVAALAESSEGASHWIKPPSGKFQTIEDLEGLLIV
                     GNPDDCAAEIEKFCARGVEHLVFDLRLQYDDYEESLELIGKELLPRFL"
     gene            complement(3097..3486)
                     /locus_tag="VFY96_15275"
     CDS             complement(3097..3486)
                     /locus_tag="VFY96_15275"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5607885.1"
                     /translation="MPLKVMRIEDAPVRVMRWDRGKALRLVDEADGAKNVDVHINLIN
                     VDSGPGPTHYPAKAENIYVVLEGNVEAIVEGKKYRLGPGEVAFIPPGVRHQAGNCGDK
                     VARVLEIYAPAGEDFHIVDDSENKPQS"
     gene            3680..4591
                     /locus_tag="VFY96_15280"
     CDS             3680..4591
                     /locus_tag="VFY96_15280"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607886.1"
                     /translation="MAETLKLIQFRAAYNLPVHVGIETGIFVRHELALEAAYTPGSLY
                     ISQALKEGEYDIGHTGADDIIAAVESDKGSALFIFMGLHSGLFSLVGAPDCASIEALL
                     GRAIGVDARTSGFALVLERMLRVRRFARNDYQLIEIGGWESRYRALSEQKIAATLLTE
                     PFVLNALKAGCHLLARDFEMISCYQGTCAAASRRFAERHPDRLIRYIQAYVEATQWCF
                     AKKNRRDCLDLLARHNQIDGPAAETTLNALLDPEHGLYPKAAVNISGVRAALEVRAEL
                     GYLTRPIPPVEKYVDLSYYHKGMIASA"
     gene            complement(4721..5317)
                     /locus_tag="VFY96_15285"
     CDS             complement(4721..5317)
                     /locus_tag="VFY96_15285"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX5607887.1"
                     /translation="MAVALGHGGACAVGGLRGVEIRVMEDLDLGALFLQFLILWFLAI
                     GGPSTIFPDIHRYVVEVHHLLTGEQFAEIYTVAQVAPGPNAMYVTLIGWYLAGWKGVV
                     ATTIPLLVPATTLTMLVGHLNERYPDAPIFRAIRRGLMPITIGLVFAGAMILMRTVNH
                     DWHGYLITFFTVAVVLRKPWNPLWLFAGGALAGILGFV"
     gene            complement(5248..5814)
                     /locus_tag="VFY96_15290"
     CDS             complement(5248..5814)
                     /locus_tag="VFY96_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020831529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX5607888.1"
                     /translation="MLDQTVAQQSLHKTAVTRLQLFIAFSRISLSSFGGAIFWARREL
                     IERQRWLTEREFLEMYALGQLLPGPNVLNLTVMVGYRFVGWSGAASAVAGFLGWPCLV
                     VVGMGILYQNYGKLPYVQEALGGMSSVAAGLLVATVIKLAAVLPRQWRPWLFGALAFI
                     GVGVMRWPLLWVMAALAPWAVFAAWKYE"
BASE COUNT         1345 a         1720 c         1577 g         1321 t
ORIGIN      
        1 cggaggagat atatatgacg aagcaaaaat tttctcgagg gttgatgacc gctgtgatcg
       61 ttggcgcctt cggcctggga tatgtttgcg gctccatgac acagcggcaa gcgcaagccc
      121 agatcggcgg gctgttggac aaggccggca aggcaggggg accggtgggc tccgtggcgc
      181 aatttggttc ttcgctggtc gagatgcagg atcacatcag cggactgcaa aaaaatctcg
      241 atacattcaa acaagtgcag gccacgctga ccggaaaata gcagctagaa tcttttgtat
      301 cagcggcact tgagttcgac tccttgccgc tcgccctgcc gtcatcgcag gtaaagccgc
      361 acaaggcaat gcagccgcgg ggctgtaccc gaaaatcgtg gatcggggga aaattcgttt
      421 ttgcttctga tcacgtcctg tttcatgtga tttatgaaat tggagcgccg gtgtaacggc
      481 tttgtcggat atcaacatga agctttcaat caaagcgatg atgatcgcag gcgcattgtt
      541 caaagccgtc gtttttctgt ttatctcgct gatgaatctc attttgcgcc cgtacggcgg
      601 cgcgtatctt gcgtttttaa gctcggtcta tcccggttat gatccgatca acgtgccgat
      661 cgctctgatc ctcggcacga tctactcatt gattgccgga gcgttcgcag gtcttctttt
      721 cggttatctt tacaatttct tcacagaacg cacttagcac gcgttgcgcg atcaatcgtt
      781 caaagactcg ggcttggtat tttgccgttg cgctcatcga gggatgtatt ttctcgggct
      841 tgcgttgtcc caattacctg atcagtccgc gatcttgatg acaattttgc cgcgtcggtg
      901 cggcgcacct tcgatcatct cgtgtccttg acgcgcctca tggagccaga ggacctcgcc
      961 gacttcggtc gctaactctc cagcgtcgat cagctcggca attttttcca atcgctcggt
     1021 tgtcacctcg acgaggaaaa acatggcgcg aacaccgtgc tgctgcgctt gttcctgtga
     1081 aggctccgcc gccgaggata cgagaattcc cccgcgcttg agcactccgt aagagcgctc
     1141 gagggtggcg ccgccaacgg tatccagcac gacatcgaca tgctttactt gctcttcaaa
     1201 ccgcttgacg cgataatcga tcacgccgta agcaccgagc ctgcgaacgt acgaagtgtc
     1261 ttttgcggcc gtagtggtaa ccaccatcgc ccccgctcgc ttcgctagct gcagcaaata
     1321 tcctcccaca ttgcccgcgc ccccatgaac aagaacgaac tgtcctggcg ccactgccgc
     1381 caattcgaat accatctgcc atgccgttac tgccacgact ggaaccgacg ccgcgtgcgt
     1441 gtggttgaga gttttcggct tgtgcgcgat catccttgct ttggcgatgg cgtactccgc
     1501 atgagctcca gtgaagcgct cattggtcac gccgaagact tcgtcgccga cattaaaccc
     1561 ccttacgccc ggtccgagag aacgaatcac acccgataaa tcagatccca aggttaatgg
     1621 caggggttgc ggcaacgcgc tcctgccgcg gcgaatcaag gcgtcccacg ctccgacacc
     1681 cgccgctttc acctgcacga cgacttcgcc gtcgcccggc tcgggtagag gtaggtcctc
     1741 gagagagatg acttcgggcg ggccataact gtgaattcga gcagccttca atcggctcac
     1801 ctaacgcgat cggataggaa tacagcgatg gcggcgcggc attgcgggcg ccgctttgtt
     1861 ggcatctcaa tcactctgga ggaaaaatga caggtcgggt cgccgtacga gttcgtatac
     1921 taatacttca caaaaacctg ggaaggagct ccttaccgat aagctctaag ctctcttcat
     1981 agtcatcgta ctgaaggcgc agatcaaata ccaggtgttc gactcctcgc gcgcaaaact
     2041 tttcgatctc cgccgcgcag tcatcgggat ttcctacgat caacaaacct tccagatctt
     2101 cgatcgtctg aaactttccc gatggcggct taatccagtg gctcgcaccc tccgagcttt
     2161 ctgccagcgc cgcaacgttt atactcgcgc gagctttctt tcggtctttg tcgatccgag
     2221 tgatcggaat attgcccaaa gtaattttcc tgtcggtcaa ttgtcgtaaa tacttgattc
     2281 gatcgtcgaa cgtcgccatg ggaacccgac cgggaatcca gccgtcacag tattctgcgg
     2341 cgcgccttat ggccgcgcgg gtgctgccgc cgcaaaaaat cgggatcggc tcgaccggtt
     2401 tgggctcgat agttacatta tcgaatgaaa atattttacc cttgtaggac gcactatttt
     2461 ccttccagat caggcgcgcg atctcgacgg tctcgcgcag gatgtcgata cgatcctcga
     2521 tgcgcaaccc gacggtggcg agctcggctg gattgaaccc caaccccatt ccggcgatca
     2581 cccggccgcc ggcaatataa gaaaggctcg ccagatcctg actgactttg agcggccaac
     2641 gcacgggaac cagcaccgcg atacccaaaa tgattttttt cgttaccgcg gccaccgctg
     2701 aaagcgtcac gaagggttcg acgaatttga gccccgccgt ttccatgccg tgcggatgcc
     2761 aaatcaagtg atctctgacc cataccgaat gaaatcccag ctcttcgcac attttagagc
     2821 cgtcgatgat ccgcgagggc gaggcttctt tgccgaaatg cggcaacagc acaccgaact
     2881 gaatttttgt catagtgctc atagaaagct gataacgatc gatcctctga acctggaacc
     2941 gctggtgaag aaaggtttct cgctgcggtc gaacgagaaa agtgacgcga agaaatttct
     3001 atcagttgtt tgagtatacg tcagttgaga aatcggctgt cgtcacgtca tttggttcag
     3061 ctctattgcg agagctttgt cgtcaaattg ctggggctaa ctctgcggct tgttttctga
     3121 atcgtcgacg atgtgaaaat cctcgcccgc gggtgcgtaa atctcgagga cgcgcgcgac
     3181 tttatcgccg caattgccgg cttggtgtcg cactccgggc gggataaacg cgacttctcc
     3241 cggccccaaa cgatattttt ttccctcgac gatcgcctcg acgtttccct ccagaaccac
     3301 gtagatgttt tcagctttag cagggtagtg ggtcggaccc gggccggaat cgacattgat
     3361 caagttgatg tgcacatcga cgttctttgc gccgtcggct tcatcgacta accggagcgc
     3421 tttgccgcga tcccagcgca taaccctgac cggcgcgtct tctatccgca taaccttcaa
     3481 tggcatactt tgtcacctcc agcttgtctt agttaactac gcgagcacta gttgaaagca
     3541 ggatcactgt caacttggct cgtgtgaaca cttgatgttt ggtctgatgg gcggttcgcc
     3601 cgcataagct ctcgttcaat gcaaggcagc ctgtcagctt tttgcgcgcc gtgataggag
     3661 gattgtgaga cttcgctcga tggcagaaac attaaaactg attcagtttc gcgccgctta
     3721 caacttgccc gtgcatgtgg gaatcgagac cgggattttt gtccggcacg agctcgctct
     3781 cgaagctgct tacacaccgg gctccctata catcagtcaa gctctcaaag agggcgaata
     3841 cgatatcggc cataccggtg cggacgacat tattgccgct gtcgaatccg ataagggctc
     3901 cgctctcttc atctttatgg gtctgcatag cgggctgttt tcgctagtcg gcgctccgga
     3961 ttgcgcctcc atagaagctc tcctcggcag agcgatcgga gtcgatgcta ggaccagcgg
     4021 cttcgctctc gtgctcgaaa gaatgctccg tgtccgacgg tttgctcgaa acgattatca
     4081 gctgatcgaa atcggcggat gggaaagccg ctatcgtgca ttatcggaac agaaaattgc
     4141 tgcaaccctg ttaactgaac cgttcgtgtt gaacgcgctt aaagcgggct gccatctgct
     4201 cgcgagagac ttcgaaatga tctcttgtta tcaaggcacg tgcgccgccg cgagtcgacg
     4261 ttttgccgaa cggcatccgg accgtttgat tcgctacatc caagcatacg tcgaagcgac
     4321 ccagtggtgt ttcgcgaaaa agaatcgccg cgattgcctc gacttgctgg cgcgccacaa
     4381 tcagattgac ggaccggcgg cggaaaccac tttaaacgcg ctactcgatc cagagcacgg
     4441 actttatccg aaagcggccg tcaatatatc cggcgttcga gccgctctcg aggtacgcgc
     4501 cgagctcggt tacctgacgc gtccgattcc gcccgtcgag aagtatgtcg acctatccta
     4561 ttaccacaaa ggcatgatcg ccagcgcatg agtggcgaaa gcaatcttca tcagcgtccg
     4621 ttttcgataa aagcccacga agcacacagc aattgccgct agattcagtc taacgtaggg
     4681 caccgaagct tctggcgcgc cccaaagcgc agttattcta ctagacaaag cccaaaatgc
     4741 cggctaaagc gccgccggca aagagccaga gcgggttcca cggcttgcgc aggaccacgg
     4801 ctacagtaaa aaacgtgatc aagtaaccgt gccaatcgtg attgaccgta cgcattaaga
     4861 tcatcgcgcc agcgaagaca agaccgatgg tgatcggcat caggccacgc cgaatggcac
     4921 ggaatatcgg agcatcggga tatcgttcat tcagatgccc aacgagcatg gtcagcgtgg
     4981 tcgcggggac cagcagggga atcgttgtgg cgaccacgcc tttccatccc gctagatacc
     5041 agccgatcaa tgtcacatac atggcgttcg gtccgggcgc cacctgcgca acggtgtaaa
     5101 tttccgcaaa ctgctcgccg gtcaacaggt gatgcacttc gacgacgtaa cgatgaatgt
     5161 ctggaaaaat tgtcgaggga ccgccaatcg ccaagaacca gaggatcaaa aactggagaa
     5221 acagcgcgcc gagatcgagg tcctccatca ctcgtatttc cacgccgcga agaccgccca
     5281 cggcgcaagc gccgccatga cccaaagcaa cggccaacgc atcacaccga cgccgatgaa
     5341 tgccagcgcg ccaaaaagcc agggacgcca ctgccgcggc agcacagccg cgagcttgat
     5401 cacggtggcc acaagcagcc cagccgccac gctggacatt ccgccgagcg cctcctgaac
     5461 atacggcagt ttgccatagt tttgatagag aatacccatg ccgacgacga cgagacaggg
     5521 ccacccgaga aaaccagcca ccgcggacgc ggcaccgctc cagccaacaa agcgatagcc
     5581 gaccatgacg gtcaaattga gaacattcgg accgggcaaa agttgcccga gagcatacat
     5641 ctcgagaaac tcacgctcgg tgagccaccg ttgcctttcg atcagctccc ggcgtgccca
     5701 aaaaattgcg ccgccaaaac tcgagaggct gatgcgcgag aaagcgatga acaattggag
     5761 tcgagtgaca gccgttttat gtaatgattg ctgtgcgaca gtttgatcca acatacaaac
     5821 ttctgttgct tcagctctat ttttaaggcg agcgcggaca cacctcaggt cgggcggagg
     5881 ccatacgccc caagagtacc ggggtcacgc gacccgcgac ggggttaggc ctatttcaga
     5941 agtttcggtc tgccaccggc ggt
//
LOCUS       DASULZ010000743         2146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_693926, whole genome shotgun sequence.
ACCESSION   DASULZ010000743 DASULZ010000000
VERSION     DASULZ010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693926"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2060)
                     /locus_tag="VFY96_15295"
     CDS             complement(<1..2060)
                     /locus_tag="VFY96_15295"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX5607889.1"
                     /translation="MQRFGDTAFLAAVVLTYAWFGTLDLQQLFNRAAPADTLISFWRG
                     GATIRSSTIVTLLIFIGAMAKSAQVPLHMWLPDSLYAPTPVHALLHAGIINAGGFLLA
                     RLAPLFDLSSLTLHIVFVVGLLTTVLGSSMMLVQSDIKKTLGYSTIGQMGFMIMECGV
                     GAYGLAIFHLIAHGIFKGTIFLNCGYVIHAARQEPRVPPNRQSAATAEFSSLTWLTGF
                     ATTLILPLIIVLAAHGIVDIPLRDSQGAAIFLFFGWATSSQAILTLYRLRAVASWKVA
                     ASMLGVLLVVLVTYLLAAENFSHFLFPAPGQVAAHFRAGALPGPAFDLLILGFALIIV
                     FGWYLIYARSHGQKLWAPGWIQTLHLRLYLFFLNRLYLDALSMRIGGFLRTAVSRLGA
                     NQAFPWIVGGIALVVSFLLAGRPPKMAPAQLGLILVSVLLLPLFPFHGIYIAALTRAP
                     GLLPFVFALLLPAAGLYALNTALPHISTALLSAGSTLALFGAVYSSLQALGQNRMTHV
                     LAYAGSAALSVAWWHLAGTGALDTHTIVYSATVSLLIAGLLLACKRAQLVSGDLTLDR
                     MHGLARPMPCLATIVALLVMGAVGLPPFALSSTVAAILLHAAVANITGLAVILFYWFL
                     ASWYLFRMMQRLLFGRHRAGASYRDLHAIELACFALLLLILAFAGIRGSWLDLQPLEK
                     VRTIA"
BASE COUNT          564 a          577 c          669 g          336 t
ORIGIN      
        1 gcgatcgttc ggactttttc gagtggttga agatcgagcc agcttcctcg gattccggcg
       61 aaggccagaa tcagcagcaa gagcgcgaag cacgccaact caatcgcgtg caaatctcga
      121 taactcgctc cggctcgatg gcgaccgaaa aggagtcgct gcatcattct gaacaggtac
      181 cacgacgcga gaaaccagta gaacaggatt acggcgagac cggtgatatt ggcaactgcg
      241 gcgtgaagca gaatcgcggc aaccgttgaa gagagcgcaa acggcggcaa gccaaccgct
      301 cccatgacca gcagagcgac aatcgtggcc aggcaaggca tcggccgcgc cagtccatgc
      361 attcggtcca gcgtaagatc gccggagacg agttgggcgc gcttgcaggc gagcagcagc
      421 cctgcgatga ggaggcttac cgtcgcgctg taaacgatcg tgtgagtgtc caaggctcca
      481 gttccggcga gatgccacca cgcaaccgaa agagccgcgg agccggcata ggccaaaacg
      541 tgcgtcatgc ggttttgccc cagagcttgc aaggaactat agacggcgcc aaagagagcg
      601 agggtgctgc cagcggagag aagtgcagtc gagatgtgcg gtaatgcggt gtttaacgcg
      661 tacaacccgg cggcaggaag caacaaagca aagacaaaag ggagaagccc aggcgcgcgc
      721 gtgagggcgg cgatgtaaat gccgtgaaag ggaaaaagcg gcaacaacag aaccgatacg
      781 aggatcagac ccaactgcgc cggcgccatc ttgggcggac gtcctgcgag taagaacgag
      841 accacgagcg cgattccacc gacgatccaa ggaaatgcct ggtttgcgcc gagacggctc
      901 accgcggttc ggagaaaacc gccgatccgc attgacagag cgtcgagata gaggcgattg
      961 agaaaaaata aatagagccg aaggtgcagc gtctggatcc accccggcgc ccatagcttt
     1021 tgtccgtggc tccgcgcata aatcaagtac cagccgaaaa caatgatcag cgcgaatccc
     1081 aagatgagaa gatcgaatgc cggcccgggc aaagcgcctg cccggaaatg ggcagcgacc
     1141 tgacccggtg ccggaaagag aaaatgcgaa aaattttccg ccgccagcag gtaggtcacg
     1201 aggacgacga gcaaaactcc gagcatgctg gccgcgacct tccatgaagc cacggcgcgc
     1261 agtcggtaaa gcgtcagtat cgcctgagat gaggtggccc agccgaagaa aagaaaaatt
     1321 gccgcgcctt gcgaatctcg cagcggaata tcgacgattc catgcgcggc caggacgatg
     1381 atcaggggca gaatcaaggt cgttgcgaat ccggtgagcc aggtaagact ggaaaactca
     1441 gcggtcgccg cgctttgtct atttggcggc actctcggct cctggcgcgc ggcatggatc
     1501 acgtagccgc agttcaaaaa aatcgttcct ttgaaaattc cgtgcgcgat cagatgaaag
     1561 atcgccaagc cgtacgctcc cacgccgcat tccatgatca tgaaacccat ctggccgatg
     1621 gtcgaataac caagggtttt cttgatatcg ctctggacca gcatcatcga ggagccgaga
     1681 accgtcgtga gcaatccgac gacgaaaacg atgtgaagcg tcagcgagct caggtcgaaa
     1741 agcggcgcca accgggcgag gagaaagccg ccggcattga tgattcccgc atgcaggagg
     1801 gcatggaccg gcgtgggtgc gtagagcgag tctggcaacc acatatgcaa tggaacttgc
     1861 gccgatttcg ccatcgcgcc aatgaaaatc agtaaggtga caatcgtgct cgagcggatc
     1921 gttgcgccgc cgcgccagaa cgatatgaga gtgtccgccg gagcggcgcg gttgaagagt
     1981 tgttgcaaat ctagagttcc aaaccaggcg taggtgagga cgacggcggc caggaaagcg
     2041 gtatcgccga aacgttgcat gatgaatgtt cgaaacgcac cgcgcacggt cgggggatgc
     2101 tgataattgt gggaaaggtt cggcagcagc cagctcaata gctgcc
//
LOCUS       DASULZ010000744         2395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_85718, whole genome shotgun sequence.
ACCESSION   DASULZ010000744 DASULZ010000000
VERSION     DASULZ010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85718"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            340..1341
                     /locus_tag="VFY96_15300"
     CDS             340..1341
                     /locus_tag="VFY96_15300"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607890.1"
                     /translation="MDMKVTSLIILTCLTLLNLSTARSAFAQLTRLKTSYSALTANMA
                     AYWLAKDAKIFEKHGLNVDVVLIESGVTTVQALTAGETQIAMGGGTVAVSSNLAGSDI
                     ISIASIESRLPYALLAQKEIKSIDPLKGKRLAVSRFGSASDLAARLILQRYGLTPDKD
                     VTLLQTGGTSTRLSALSKRSIDCTVLTPEFFNVGKKAGFTILVDPTQYDIPFPQLEVI
                     TSRAFLKAQPDLATRYLRAIIEGIHMVKNNPEPSIRAMSKYLRIDDREALEEVYRLYK
                     EIYPQSPYPSAPAIQTQLTWMAERDPRARAAKPEQFIDGTLLREIEKSGFVAKLYQK"
     gene            complement(1445..>2395)
                     /locus_tag="VFY96_15305"
     CDS             complement(1445..>2395)
                     /locus_tag="VFY96_15305"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX5607891.1"
                     /translation="GGAGAALAAGLLPLASGSDLGGSLRNPANFNNIVALRPTVGLVP
                     SAPTALPFVGFSVKGPMGRSVEDVAFLLSTMAGADPRDPAVYPSDPHVFAEPLMRDLK
                     NIRLAWSLDLDSLPLDNRVRSILEAQRKTFSDLGCTVEDASPDLAGADEIFLTIRAWS
                     YWHTLGPLLKEHRQQMKPEAIWQIESGSKLSGSDVAKAMAQHGELMQRMRLFQEKHEF
                     LVCAVNQLPPFDANIHWPREIDGVPMEHYIAWMKSAYWITPTFCPAISVPAGFTAEGL
                     PVGIQIVGRYRDDLGVLQIAHAFEQATGFGKKRPKIALGP"
BASE COUNT          549 a          700 c          623 g          523 t
ORIGIN      
        1 cttccagaaa atcgtgcccg gttcgagggt gcatcgcttc atgcactccg cccatgagcc
       61 gcaaatcgaa gagtccggcg agttcaaccg agtgttgacg gaatttctgc tcggctaatg
      121 gcgtcagcaa tagggctcgg cctgcttcga cacttcgact ttgctcagtg cgggctccct
      181 cgactgcacc ttcactcccg agcttgtcga ggaatcagca tgcacggatt tttgtcagat
      241 cttctcgttt ttacgctcat tctgagcatg tcgaagattc cgaaaggctt ttcagccatc
      301 tgttagactt cgctgtggca atgagatgag gagaactcca tggatatgaa agtgacctcg
      361 ctaataatat tgacctgctt gacactgcta aatctgtcca ccgctcgatc cgccttcgcc
      421 caactcaccc gcctgaaaac ctcttatagc gctctcaccg ccaacatggc ggcctactgg
      481 ttagccaagg acgcaaagat ttttgaaaag catggattga acgtggacgt cgtgctcatc
      541 gaaagcggtg tgaccaccgt gcaggcgctc accgcaggtg aaactcaaat cgcgatgggc
      601 ggtggcaccg tcgctgtcag cagcaatctg gccggaagcg atatcatctc catcgcgagc
      661 atcgaaagtc gtcttcccta tgccctgctc gcccaaaaag aaatcaaaag catcgatccg
      721 cttaaaggta aacggctggc ggtttcccgt ttcggcagcg cttcggatct cgccgcgcgt
      781 ctcattttgc agcgatacgg tttgactcca gacaaagacg tcaccttatt gcaaaccggc
      841 ggcactagca cccgtctctc cgctctcagc aagcgcagca tcgattgcac ggtgctgacc
      901 ccggaattct ttaacgtagg gaagaaagcc gggttcacga ttttggtcga tccgacgcaa
      961 tacgatattc cttttcccca gctcgaagtg attaccagcc gcgcttttct taaagcacag
     1021 cccgatctgg ccactcgcta tttgcgcgcg atcatcgaag gcatacacat ggtcaagaac
     1081 aatccggagc ccagcattcg ggccatgagc aaatatcttc gcatcgacga tcgtgaagcg
     1141 ttggaagagg tttatcggct ctacaaagaa atctatccgc aaagtcccta tccgtcggca
     1201 cccgctatcc agactcagct cacctggatg gccgagcgcg acccacgcgc ccgtgccgcc
     1261 aagccagagc agtttatcga cggcacactt ttacgcgaaa tcgaaaagag cggttttgtt
     1321 gcgaagctct atcagaaatg atccatgtct ttgtaggtcg gttagcaaag cgtaacccgg
     1381 cattccggta aaccgatcga aacgcaaatt ggtcaaaaac gttgggttac gccgcgtaac
     1441 caacctacgg gccgagcgca attttcggcc gtttttttcc aaacccggtt gcctgctcga
     1501 atgcgtgggc gatttgcaat acaccgagat cgtcgcgata tcggccgacg atctgaatgc
     1561 ccacaggaag accttccgcc gtaaaacccg caggcacgga gatggccgga caaaaagtcg
     1621 gcgtgatcca gtaggcgctc ttcatccagg cgatgtagtg ctccatcggc acaccgtcaa
     1681 tttcccgcgg ccagtgaata ttcgcatcga acggcgggag ttgattcacg gcgcagacga
     1741 gaaactcatg cttctcttga aataatctca tgcgctgcat caattcaccg tgttgcgcca
     1801 tcgccttggc gacatcgctt ccgctgagct tgctacccga ttcgatctgc cagatcgcct
     1861 ccggtttcat ctgctgccga tgctctttca gaagcggccc gagcgtatgc caatagctcc
     1921 atgctcgaat ggtcaaaaat atttcatcgg cgccggccag atcgggagaa gcgtcctcga
     1981 cggtacagcc tagatcgcta aatgtttttc gctgcgcttc gagtatcgat cgtactctgt
     2041 tgtcgagcgg caaactgtcg aggtcgagag accaggccag ccgaatgttt ttaaggtcgc
     2101 gcatcaaagg ttctgcaaac acatgaggat ccgaaggata cacagcagga tcgcgcggat
     2161 cggcgccggc catcgtgctc agcagaaaag cgacgtcctc aacggatcgc cccatcgggc
     2221 ctttgacgct gaagcccaca aacggcaacg ccgtcggtgc ggatggaacc aggccgaccg
     2281 tcggtcgcag cgcgacgata ttattgaagt tggccgggtt tctgagcgag ccaccgagat
     2341 cgctgccgct tgccaagggt aagaggccgg cggccagtgc ggcgcctgca ccgcc
//
LOCUS       DASULZ010000745         3408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_897787, whole genome shotgun sequence.
ACCESSION   DASULZ010000745 DASULZ010000000
VERSION     DASULZ010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897787"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VFY96_15310"
     CDS             <1..344
                     /locus_tag="VFY96_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX5607892.1"
                     /translation="LYRFGIQAIVGESFAAIFAGNCTMMGLPTVTVGAKEIEQLMKDV
                     EDNPRTEYTVDLEQKTISYGSQRFAIDLPHTYCMALTQGHWDSTALLRANLDRVKKIA
                     AKLPYMSGFNG"
     gene            590..898
                     /locus_tag="VFY96_15315"
     CDS             590..898
                     /locus_tag="VFY96_15315"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEX5607893.1"
                     /translation="MMANKWDERKKAKEDEYFVKRERELLAKLKLKQEAEAKEVARKD
                     SAMKCPKCGAPLKERSFQKIQIDQCTGCNGIWLDAGELEQVAAKENASWLGKFWQKNG
                     "
     gene            922..1494
                     /locus_tag="VFY96_15320"
     CDS             922..1494
                     /locus_tag="VFY96_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016877928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5607894.1"
                     /translation="MTRSRKFMRGVLALVLFVAFTLLGKSGVAQEGKIVPYVPTPQEV
                     VDRMLELAELKKGDVVYDLGSGDGRIVVTAAKKYGVKAIGFEIDPQRIKESHENIKKA
                     GVENLVEIRQQDIRTVDLSAASVLTMYLLPEVNLMIRPNIWKQMKPGSRVVSHDFDMG
                     DWKPLKTEQIKDGTSWEHTLYLWHVETGRK"
     gene            1503..2666
                     /locus_tag="VFY96_15325"
     CDS             1503..2666
                     /locus_tag="VFY96_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607895.1"
                     /translation="MGFSGRPIRSRALLFILLFGVLPNTATAQIKIGIAEALTGNASQ
                     YGVAIRKGFELAAGEIASSGDINGQKLELLIEDEQGKKEEAINVFKKLIFQDKVLLLF
                     GPTLSNSAQASDPVAQAAKVVVFGTSNTADGITSIGNYVFRNSVTEADILPVTLKLAA
                     QKTGFKKVAVLYGNDDIFTRSSYDNFKKALQDLKIGVTTTETFAKGDVDFKPQLTKIK
                     GTNPDAIVLSALVAEGAPIMVQARQLGMTLPFVGGNGMNSPRIFELAGNHSDNLWVGS
                     PWSVESSTPENKRFVAAYKKTYGALPDQFAAQAYDAMYIAAEAMRKVKFAGNLDANRK
                     ALRDALPAVRWNGATGPFKFRQASGRNGKPAGYDAVQTAIVMVTKRNQYVIEK"
     gene            2670..>3408
                     /locus_tag="VFY96_15330"
     CDS             2670..>3408
                     /locus_tag="VFY96_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5607896.1"
                     /translation="MLAQQIINGLSLGGVYALFALGFTLIFGVLNVINLAHGAIFMIG
                     AYAALHAVLVFHFSLLPAIFVAICVTGAAGWILDKFILAPLRARRAPHLAPMIATIGV
                     AICLTSLVEGYFGSENLRFPLDTLPSSTVTLASVQVNLLDLRIILLALMLMVVMLWII
                     RHSAMGRALRGVAESPKAAALLGVNVEGLFLATSVIASILGGVAGVLIALYTNSVFPH
                     MGQPMLHKGIAVVIIGGMGDVRGALIGG"
BASE COUNT          812 a          877 c          978 g          741 t
ORIGIN      
        1 cgctctaccg gttcggcatt caagcaatcg tcggggaatc ttttgcggcg attttcgctg
       61 gcaactgcac gatgatggga ctgcctacgg tgaccgtcgg cgcaaaagag atcgagcagc
      121 tcatgaaaga cgtcgaggac aatccgcgaa ccgagtacac cgtcgacttg gaacagaaaa
      181 cgatttccta cggaagccaa cggttcgcca ttgacctgcc gcacacctat tgcatggcat
      241 tgacccaggg acattgggat tccactgcgt tgttgcgggc caatctcgat cgggtaaaga
      301 aaatcgcggc aaagctgccc tatatgagcg ggttcaacgg ctagcaaaac actgccgtct
      361 cccctctgcc gtcgcaagcc accacatgcc tgaaagaatc gtcttaacgc acgccacgca
      421 taggagcggc agggaagtca tcgacgactc tccttctcgt ccaaccttgg cggcgaagga
      481 ccggcaatcg tgctgattca cgggctgccg agaagtcagt cacccgaaca gccaagtggc
      541 tgagaaaggt gtcagcttaa cagcgctgat ccgaaacagt gggagaatca tgatggcgaa
      601 caaatgggac gaacggaaga aagcaaaaga agacgagtat ttcgtgaagc gggagcgcga
      661 gcttttagcc aagctgaaac tcaagcagga agcggaggcc aaggaggtgg cgaggaaaga
      721 ttccgcgatg aaatgcccta aatgtggtgc acccctgaag gagcgaagct ttcagaagat
      781 tcaaatcgac caatgcactg gctgcaacgg catttggctg gacgctggcg aactggaaca
      841 ggtcgccgcc aaggaaaacg ccagttggct cggaaaattc tggcaaaaga acggttaact
      901 caccagagag caggaggatc tatgactaga tcaagaaagt ttatgcgagg agttctggcg
      961 ctggtgcttt ttgttgcgtt cactcttctg gggaagtcgg gcgttgcgca ggaaggcaaa
     1021 attgtgcctt acgttccgac gccgcaggaa gtcgtcgacc gaatgcttga attagccgaa
     1081 ctgaagaaag gtgacgtggt ttatgatctc gggtcgggtg acggccgaat cgtcgtcacc
     1141 gcggcaaaaa aatatggtgt taaagcgatt gggttcgaaa ttgacccgca gcgcatcaaa
     1201 gagtcccatg agaatatcaa aaaggctggt gtcgagaact tggtcgagat tcgccaacag
     1261 gacattcgca ccgtggattt gtctgcagcc tccgtactga ccatgtatct tctgcccgag
     1321 gtcaatttga tgattcgccc gaacatttgg aaacagatga aacccggatc gcgtgtcgtt
     1381 tcgcatgatt tcgatatggg cgattggaaa ccgctcaaaa ccgaacagat caaagatggc
     1441 acgagctggg agcatacgct ctatttatgg catgtcgaaa cgggccgaaa atagccgctg
     1501 cgatgggatt tagcgggcgg cccattcgtt ctagagcgtt gctcttcatc ctcctcttcg
     1561 gggttctgcc gaacacggct accgcccaga ttaagatcgg gattgcggaa gcgcttaccg
     1621 gaaacgcgtc ccaatatgga gtggcgatcc gcaaagggtt tgagctcgcg gccggtgaga
     1681 ttgcttcgtc gggcgacatc aatgggcaaa aattggagct cctgatcgag gatgagcagg
     1741 gtaaaaaaga agaagcgatc aatgttttca agaaactgat tttccaagac aaggtcttac
     1801 tgttgtttgg gcctactctg tcgaactccg cacaggcctc agacccggtg gcgcaagcgg
     1861 ccaaggtggt tgtcttcggt acgtcaaaca cggcggacgg gatcacttcc attggcaatt
     1921 atgtgtttag aaattcagtc accgaggccg atatcttgcc cgtaacgttg aaactcgcgg
     1981 cgcaaaaaac cggtttcaag aaagtcgcag tgctttacgg caatgacgac atcttcacca
     2041 ggagcagcta cgacaacttc aaaaaagctc ttcaagatct gaagatcggc gtgaccacta
     2101 cggaaacctt cgccaaaggc gacgtcgact tcaaaccgca gttgaccaaa atcaaaggga
     2161 ccaatccgga tgccatcgtc ctatcggcgc tcgtcgccga gggcgcgccg atcatggtgc
     2221 aggcgcgaca actcggcatg acgctaccgt ttgtcggcgg taacggcatg aattcgccgc
     2281 gaattttcga gctcgcggga aatcattcgg ataatctctg ggtcggaagt ccgtggtcgg
     2341 tcgagagttc gacgccggaa aacaaacgct ttgtcgctgc atacaagaaa acctatggcg
     2401 ccttgccgga ccagtttgca gcgcaggcat acgatgcaat gtacattgcg gctgaggcca
     2461 tgcggaaagt taaatttgca ggtaacctcg acgcaaatcg taaagcgctg cgcgacgctt
     2521 tgccagctgt ccggtggaac ggcgccaccg ggcctttcaa gtttcgccag gcgagcggcc
     2581 gcaatggaaa gcccgctggc tacgacgcgg tgcaaaccgc gatcgttatg gtaacgaagc
     2641 gcaatcagta cgtcatcgaa aaatagcgca tgctggcgca gcagatcatc aacgggctgt
     2701 cactcggcgg ggtgtatgcg ctattcgctc tcggttttac actgatcttc ggcgtcctga
     2761 acgtcatcaa cctggcgcac ggcgcgattt tcatgatcgg cgcgtacgcc gccttgcatg
     2821 cggttctggt ctttcacttc tcattattgc ccgcgatttt tgttgctatc tgtgttaccg
     2881 gcgctgccgg ttggattctc gataaattca ttttggcgcc attgcgagcg cgccgagcgc
     2941 cccatctggc gccgatgatc gcgacgatcg gcgtagcgat ctgtctcacc agcttggtcg
     3001 aaggctattt tgggtcggaa aaccttcgtt ttcctctgga tactttacca tcaagcaccg
     3061 tgaccttggc cagtgtgcag gtgaatttgc tcgatctcag aatcattctt ctcgcgctta
     3121 tgctgatggt tgtgatgctc tggatcatca ggcacagcgc tatgggacgc gcgcttcgcg
     3181 gtgtggccga gtcgccgaag gcggcggcgc ttctcggagt caatgtcgag ggcttattcc
     3241 tcgccacttc ggtgatcgcg tccattctcg gaggcgtggc aggcgtgcta attgcgcttt
     3301 atacgaactc cgtatttccc catatgggac agccgatgct acacaaaggc atcgcggtcg
     3361 ttattatcgg cgggatgggc gatgtgcgcg gcgcactgat cggcggtt
//
LOCUS       DASULZ010000746         4619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1098796, whole genome shotgun sequence.
ACCESSION   DASULZ010000746 DASULZ010000000
VERSION     DASULZ010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098796"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VFY96_15335"
     CDS             <1..541
                     /locus_tag="VFY96_15335"
                     /inference="COORDINATES: protein motif:HMM:NF020071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5607897.1"
                     /translation="ILADEVVAGRLNLDNWTDAVEQWILRVKRLREWMPDLELPVISD
                     DDRATIIEHICHGAASYGEIEDRAVLPVVKSWLSQQQQKWVDEYAPERIQLPNGRMVK
                     VAYTPDAPPLIAARIQDLYGVKEGLWIAQRRVPVRIQVLAPSNRPVQVTENLSLFWRE
                     TYPKLKTQLQRRYPKHEWR"
     gene            complement(807..1934)
                     /locus_tag="VFY96_15340"
     CDS             complement(807..1934)
                     /locus_tag="VFY96_15340"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5607898.1"
                     /translation="MTAKISVLLFFILCYRSTSFAAEAKPDWRAEWDKTVAAAEKESI
                     VSIYIFENGPLTEETVRAFERAYPKIKVSQLRGRGNDLGPRLVAERRAGKHIADLFAG
                     GKGTAFSTLYVGKLLDPIKPILLLPEVLDESKWWRGSHKYVDPENKYIFVYVGNAGGV
                     EINYNSKLVNPREFTSYWDLVQPKWKNKIVAVDPRMRGMDNPVLFFYYHAKLGPDFIK
                     KLYGEMEVSITRDYRQPIDWLAAGKFSLCLPCVSDEMDKAMEHGLPVGQILNLKEGGT
                     LSSSGGTLSLLQNAPHPNAAKIFVNWLLSREGQTEVQKGRKGRPRTGSNSLRIDIPKT
                     NLPEEIQRKDGVEYFDADDDSNADRRPADRLFNEIFGKPSR"
     gene            2220..2354
                     /locus_tag="VFY96_15345"
     CDS             2220..2354
                     /locus_tag="VFY96_15345"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5607899.1"
                     /translation="MTFTKREHKTPEFLKINPAGKLPALLSGDVKVFESAAICLYLAR
                     "
     gene            2481..2693
                     /locus_tag="VFY96_15350"
     CDS             2481..2693
                     /locus_tag="VFY96_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607900.1"
                     /translation="MPQRGWQQFETVKDVIEGELGDEPYLFGDWFKAADVMIGRCSSG
                     SECGVSHPGGPSWKPTSIVSSPAQRQ"
     gene            2868..3516
                     /locus_tag="VFY96_15355"
                     /pseudo
     CDS             2868..3516
                     /locus_tag="VFY96_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739608.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
BASE COUNT         1061 a         1245 c         1242 g         1071 t
ORIGIN      
        1 gattctggct gacgaagtcg tcgcgggccg gttgaacctc gacaactgga ccgacgccgt
       61 cgagcaatgg attctccgcg ttaagcgact gcgcgaatgg atgcctgatc tcgaactgcc
      121 tgtgattagc gacgatgatc gcgcgacgat catcgagcat atttgccatg gcgcagcgag
      181 ctatggcgag atcgaagatc gcgcggttct gcccgtggtc aagtcatggt tgtcccagca
      241 gcagcaaaaa tgggtcgatg aatatgcgcc cgaacgtatt caactgccga atggaagaat
      301 ggtaaaggtc gcgtatactc ccgacgcgcc gccgctcatc gcggcgcgca tccaggattt
      361 atacggcgtc aaagaaggcc tgtggatcgc gcagcgacga gttccggtgc ggattcaggt
      421 gctcgcgccg agcaaccgcc cggttcaggt caccgagaat ctgtcactct tctggcgcga
      481 aacctatccc aagcttaaaa cgcaattgca gcgccgttat ccgaaacacg aatggcggtg
      541 accggcatga gtcttgtcat gacgacaata ttttcggcta agtgaactaa acgcgaggca
      601 acgctctctt cccggcgtga gcatcgagga gtcgagaacc acagattgtc atttcgacga
      661 acgggagaaa tctttccgta acccaaaagc gaacgtaaaa gaaaaggata atcttgtaag
      721 actcggtcac cagaacagtc ttgagcaaca tccaaattgt tctgcagggg gacctgagcg
      781 gattggtcgt tattcgaccg actaggctac ctgctcggct taccgaaaat ttcgttaaac
      841 aacctgtcgg cgggccgtcg gtccgcgttg ctgtcatcat cggcgtcgaa gtattcgacg
      901 ccgtccttac gctgaatctc ctcgggaaga ttagttttgg gaatgtcgat ccgcaacgag
      961 ttagagccgg tgcgcggccg ccccttgcgt cccttctgaa cttccgtttg tccttcacgc
     1021 gacagcagcc agttgacgaa aatcttggcg gcattcggat gcggcgcgtt ttgtagcaag
     1081 ctcaaggtgc cgcccgacga gcttaaagtg ccgccttctt ttagattcag aatctgaccg
     1141 accggtaaac catgctccat cgctttgtcc atctcatcgg agacgcacgg aagacacagt
     1201 gagaactttc ccgccgcgag ccagtcgatg ggctgacggt aatcccgtgt aatggacact
     1261 tccatttcgc cgtaaagctt tttgatgaag tcgggcccga gcttggcatg atagtagaag
     1321 aacaacacgg gattatccat gccgcgcatg cgcggatcca cggcgacaat cttgtttttc
     1381 catttgggct gcaccaggtc ccagtaggaa gtaaactcgc gcggattgac cagtttcgaa
     1441 ttgtaattga tttccactcc gccggcattg ccgacataaa caaagatata cttgttttcc
     1501 ggatcgacgt atttatggct accacgccac catttgctct catccaatac ctcaggcagc
     1561 agcagaattg gcttgatcgg atcgaggagc ttgccgacat aaagcgtgct gaacgccgtt
     1621 cccttcccgc cggcgaaaag atccgcgata tgctttcccg cgcgccgctc tgccacgagc
     1681 cgcgggccga gatcattgcc tcggccgcgg agctggctga ctttgatttt cggataagcc
     1741 cgctcaaaag cacgaaccgt ttcctcagtg agaggtccgt tttcgaagat gtaaatgctg
     1801 acgatacttt ccttctcagc cgcggcaaca gttttgtccc actcggcgcg ccagtccggc
     1861 ttcgcctccg cagcgaagct cgtactgcga taacaaagaa tgaaaaacag caataccgat
     1921 atttttgcag tcacgttttc gcgcgattca gcgccgccta ctgtaaaagc ggtggcgtat
     1981 gagtgtcaac atgattgcac ggttctgacc agtctgaagc cagcaggcta gcaaattcag
     2041 cagagcattg attagtgctc ttgaatgctt cgttcagttg atttcgcttc ggttccttct
     2101 caaactgatc gagtccgggt tccaattact tcctttgagg tgtccgatga agctctattt
     2161 caatccgcag agccgcgcgg taatcgctaa atggatgctc gacgagtgcg gagtccaata
     2221 tgacgtttac caagcgcgaa cataaaacac cggagtttct caagatcaat ccagcgggaa
     2281 agctccccgc cttgctctcc ggtgacgtga aggtattcga aagcgcggcg atctgtctat
     2341 atcttgcccg ataagttttc tgaagcgaac ctcgcgccga aaatagaggc gccccaacgg
     2401 ggacgctatc tctctttgat ggtttactcg acttcccagc tcgagcccgc catgggcgac
     2461 gctctattga atgtaaaaca ttgccgcagc gcgggtggca gcaattcgag acggtcaaag
     2521 atgtgatcga aggcgagctc ggcgacgagc cctatctttt cggcgactgg ttcaaggccg
     2581 ccgacgtcat gatcggtcga tgttcatctg gcagcgaatg tggggtaagc catccgggcg
     2641 gcccaagctg gaagcctaca tcgatcgtct cctcgcccgc ccaaaggcaa tgaagctcgg
     2701 caagtaagcg cgaatagaac tattcgtgat cgactatcac ctcaaattcg gcgggagtaa
     2761 acgtcatacg attctgatca ctgcgtgtat gtccgtgact tgtcctgaga aggcttcctg
     2821 gttcgtcgta ttattaaagg tagcgtcaga aggagttcga caggaagatc aagctctacg
     2881 agttcggccc cactcgatcg attcgggtgc gctggacgtt cagaaattag gcttagagtt
     2941 cgactccgtg cgtgtcaacc tgctggcggg cgagcaccgc cgtcccgagt tcctcaaaat
     3001 caaccctgcc ggaaagattc ccgtgcttgt cgacgacgat atggtgctca cggaatccgt
     3061 ggcgatcgtg ctctacctcg cggaaaaata tcccgagaag gagctgctgc ccgccggtat
     3121 cgaagtgcgc gcacaggtta atcgatggct tctgtttgcc gcgaccgagt tggaacagcc
     3181 gctctggcgt atttcccgcc atacgacgct gtatcccgaa gaggaacgcc tgccgaaaga
     3241 agtgctgctc ggcggcaggg atttcaaaga gatggcggcg gtgctggaaa agcacatgca
     3301 aggacggcaa ttcgtggtcg gagataacgt gacggtcgcc gatttcgtga cggcttacac
     3361 aatcgactgg ggcaatgagg ttaaattgct cggcgattga cgcagctcat ggcctacatg
     3421 gagcggatgt atgcgcgccc gcttgcgccg ccgcgcatcg ctcaagcgtt cgcggcaatt
     3481 tcatcgaaag aaaattccac tgccggcagt ggataactgc cgaactgcag cgcattaccc
     3541 gaaaccggag ctcgtcgagg caaagctcat acgagtcggg cgacatcctg cttcgctacc
     3601 ggcggccaaa ttagccaagc cgtgccgtgt accagtagat gtttcgcaat cccgaaacgt
     3661 gctacagaag ccgatggaag aagattgccg cgcgccgtcg ggctccgtga ggccactcgg
     3721 catccggttt tattggcaat cgacaacggc tgccgataac aaatcgtccc acccggccag
     3781 tatcgagctt ttcatttcga ccggaccgaa tgattgcttg acacgcgaat tcgaccttac
     3841 attgagacca agagccaatt ccaaagcgct ggggtcaatt ggaactgtta caaatgaacg
     3901 cggtcggttc tttgtaagcc aatcttcagc gcttcgagga tggaaagagc aagctcgaat
     3961 cgtttcagga aatcactgcc aagttgactg agatacgagg cgcgagcgag gtaactagcc
     4021 ctctagctca gcttacaacc tgcgaatcgt tcggttactg atctctatca ggctgctttt
     4081 acctgggggt gaggggctcc ctttgtttag actgatcggt gtcttttccc tttgtcgtat
     4141 ccgaggctga gttcttggta ctgccagacg cggatttttt tgtcgttgta tcagaaggct
     4201 gtggcgtcgc catatcgctg gaccgacttg tgcctttctc gaccgtgcca gtggatggat
     4261 cacctttcgg tagcggtacc cccgattggt tttgctctga tttagagccg cctttggact
     4321 catttaactc ggggtggatt gtttcaccgc tcttgcctga agtctgagcg acagcaatcc
     4381 cgccattcat ccatgccgcg agcaaaatag atcctgatat cagcaatcgg ctgtgttcca
     4441 tatctacctc cttaactaga atggcaacca agcacggcaa gggttatgcc atgcgcgaga
     4501 atcaagtaaa aacaatgaaa ttacgtttct cttctcatag cgcttaaagc attcaacgcc
     4561 tagctgaagg aaatccaagg aatagttctt gagattgtgt ggattttcgc acgccgttc
//
LOCUS       DASULZ010000747         2278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_795415, whole genome shotgun sequence.
ACCESSION   DASULZ010000747 DASULZ010000000
VERSION     DASULZ010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795415"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..743)
                     /locus_tag="VFY96_15360"
     CDS             complement(177..743)
                     /locus_tag="VFY96_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013045210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5607901.1"
                     /translation="MMKRDEMLKTFARQRKDEIVVAVYTAAQELTHIQPSDLNYTFVG
                     AMGQGSSHALGLALGRPDKKVVVFDGDGSLLMNLGSLVTIANQSPKNYVHCVCENGCY
                     ETNGSVPIPRAGQIRFAELAKSAGYKKTYEIADLPEWERKLPEILQDEGPVFVTLKVE
                     AGEAYPENFPRLYSLEHREKFRQALARS"
     gene            complement(746..1255)
                     /locus_tag="VFY96_15365"
     CDS             complement(746..1255)
                     /locus_tag="VFY96_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HEX5607902.1"
                     /translation="MADYPLAAQTIKNEFRRAGIRFVVALPDRVTSHYLLKGLIADPE
                     FKVVQVCKEDEGISICSGLYAAGQKSVFMMQYTGFLDSINAIRGVAVEGHFPVCMIVG
                     LLGKEPDVAPTKSKKYGVKIIEPILDVMGIEHHLIECDSDCAQIGQAIDKAFANSSPA
                     AILIGREPR"
     gene            complement(1296..1658)
                     /locus_tag="VFY96_15370"
     CDS             complement(1296..1658)
                     /locus_tag="VFY96_15370"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX5607903.1"
                     /translation="MLEHPDDLKAMVEAMQFIDELVRRLEIKEFYGPLIQPSAEEDWG
                     EFARKTYDSYHHGVGTCKMGPASDAMAVVDQRLRVHGMQNLWVGDASIMPVVSRANTN
                     LTSLMIGERLADFVQEVA"
     gene            complement(1662..>2278)
                     /locus_tag="VFY96_15375"
     CDS             complement(1662..>2278)
                     /locus_tag="VFY96_15375"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5607904.1"
                     /translation="DLNVPEPYGVCASPYNIKDGKRQSAVVAYLDQARGRKNLTIVDE
                     ATVHSLKLSGKKAEGVNYEKNGAMQTALGGQIVLTSGVYGSPQILMLSGIGPSAELKR
                     NGIPIIHELNGIGENYQDHPVVFMTYEGPKESKVDWVVPRFRLIIRKNPISEAANFHI
                     NMRPPTEVTGLKRMMPISAHLLEQRNRGRVFLESADPHDQLGID"
BASE COUNT          502 a          649 c          592 g          535 t
ORIGIN      
        1 tgacaaatac gggctgctcg cgaatttgcg acacagtccc ttacggaaga aggcaaggat
       61 aaggtggatt aaagaaccac cctcatctta gtcgcggtag aggaaacaaa gaaacggaag
      121 gttcgcgcag aatttcacga aggagaaaag actccgtgtg tttgaagact cgaagtttag
      181 cttctcgcca aagcctggcg aaatttttcc cggtgctcga gactatagag acggggaaaa
      241 ttttccgggt aggcttcgcc agcttcgact tttagggtca cgaagactgg gccttcatcc
      301 tgaagaattt ccgggagctt cctttcccac tccggcaggt cagcaatctc ataggttttt
      361 ttatagccgg cgctcttggc gagctcggca aagcggattt gccccgctcg cggtatcggc
      421 actgaaccgt tggtttcata gcagccgttt tcgcagacgc aatggacata attttttggc
      481 gactgattcg cgatcgtgac caggctgcca aggttcatta ataaactacc gtcgccatcg
      541 aataccacaa cttttttgtc gggccgcccg agagcaaggc caagcgcatg cgatgagccc
      601 tgccccatgg cgccaacaaa cgtataattc aaatcactcg gctgaatatg cgtcagttcc
      661 tgcgccgccg tgtaaaccgc gacgacaatc tcgtccttgc gctggcgcgc gaacgtcttg
      721 agcatttcat ctcgtttcat catggctagc gaggctccct tccaatgaga attgccgccg
      781 gtgaggagtt ggcaaacgct ttatcgatcg cctgcccgat ctgtgcgcaa tcgctatcgc
      841 actcgatcag atgatgttca atccccatca catcgagaat cggctcgatg atcttgacac
      901 catacttttt cgatttggtc ggcgccacgt cgggttcctt gccgagtaaa ccgacgatca
      961 tacacacggg aaaatggcct tccaccgcca caccgcggat tgcattgatc gagtcgagaa
     1021 agccggtgta ctgcatcata aaaaccgatt tctgtcccgc ggcataaagg ccgctacaaa
     1081 tcgagatgcc ctcgtcttcc ttgcacacct gcactacttt gaattccgga tcggcgatta
     1141 accctttgag caagtagtgg ctcgtgacgc gatcgggcaa ggctacaacg aaacggatgc
     1201 ccgcgcgcct gaattcattt ttaatcgtct gcgccgctaa tgggtaatcc gccatattcc
     1261 ctccatgaaa cggtaggacc ggagcatgct gcgccctatg cgacctcctg cacaaaatcc
     1321 gccagccgtt cgccgatcat gagcgaagtc aagttggtat tcgcccggct caccaccggc
     1381 atgatcgagg cgtcgccaac ccaaagattt tgcataccgt gtacgcgcag tcgctgatcg
     1441 accacggcca tcgcatcgga agcgggaccc attttgcacg tgccaacccc gtggtgataa
     1501 ctgtcgtacg tcttgcgggc aaattcgccc cagtcttctt cggcgctcgg ttggatcagc
     1561 ggtccataga attctttgat ctccagacgt cgcaccagtt catcgatgaa ctgcatcgcc
     1621 tcgaccatgg ccttcaaatc gtcgggatgc tcgagcatgc gctagtcgat gccgagctga
     1681 tcgtgcggat ccgcgctctc caagaaaacc cggccgcggt ttcgctgctc gagcaaatgc
     1741 gccgagatcg gcatcatgcg cttcaagccg gtaacctccg tcggcgggcg catgttgatg
     1801 tggaagttcg ccgcctcgct gatcgggttt ttgcgaatga tcagtctaaa gcgcggcacg
     1861 acccaatcca ctttggattc cttcggacct tcgtaggtca tgaatacgac gggatgatcc
     1921 tgataatttt cgccgatgcc gttgagctca tgaataatcg ggatgccgtt cctcttgagc
     1981 tcggcggatg gaccgatgcc ggagagcatt agaatttgcg gcgagccgta aactcccgag
     2041 gttagcacga tctgcccgcc cagtgccgtt tgcatcgcgc cgtttttctc gtaattgacg
     2101 ccctcggctt ttttgccgga gagcttgagc gagtgcacgg tcgcctcatc gacgatcgtt
     2161 aaattttttc ggccgcgcgc ttgatccaaa taagcaacca ccgcggactg tcgcttgccg
     2221 tctttgatat tgtaaggtga cgcgcaaacc ccataaggtt ccggcacatt gagatcgg
//
LOCUS       DASULZ010000748         5409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_288528, whole genome shotgun sequence.
ACCESSION   DASULZ010000748 DASULZ010000000
VERSION     DASULZ010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288528"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1553)
                     /locus_tag="VFY96_15380"
     CDS             complement(<1..1553)
                     /locus_tag="VFY96_15380"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5607905.1"
                     /translation="MLRLPPQVSRWFSEPTALNAYLVRLIFVGLVPILVFSVFMMVLF
                     ARQEQANRQRGLEDTARALALAIDREIESSITNLRALATSESLDVGAVNVFRSVAARI
                     LRTQKSWKSISLFDPQGRQLAQMTKPLVDTPKGISRESLDAVLRTRRPFISDFPQVDS
                     PESGVNIHVPVVREQTIIYILTAAIEPQVFTDILLQQKLPPSWVGTVYDSQYITIART
                     RDAAKYLGRPVGSLLQQTGIERSEQFLSGITADQIRAYAAISRSHVSNWFVALTVPSA
                     EVNAILYRSLATLGAGGLLLMLIGLAAALIFARRVAYSIGELAAAAHRLGRGEAAALT
                     GKLPVAELDSLAVEMARAGELLADREKERDRVEIALRQHEQFLRRQADLLNFANEAIL
                     AWELRGPIIYWNHGAEQLYGYAQHEALGRSSHELLQTEFPNGRAQFQAELVRRGEWSS
                     ESKQVTKHGRRIEVESRFKLIEDRAGSRLVLECNRDVSHRKQSARRLATEHAVTLVLA
                     ESETPEVAWR"
     gene            complement(1667..2095)
                     /locus_tag="VFY96_15385"
     CDS             complement(1667..2095)
                     /locus_tag="VFY96_15385"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5607906.1"
                     /translation="MENEKSARSNQDGTSESLPKAVDTIRQSVLVVDDEENFLTLLKW
                     FLTQRGYDVSTALSADEALDMVQTQSFDVALLDIKLGSGDGLTLLEQVTARSPRLKVI
                     MMTAYPTVASLKQAFSKGAMRYLTKPVDLQELTEALRLLV"
     gene            complement(2431..3201)
                     /locus_tag="VFY96_15390"
     CDS             complement(2431..3201)
                     /locus_tag="VFY96_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607907.1"
                     /translation="MLNLINDSQIPYLVGGGYALDHYTGIVRNSKDLDLVLEPAHAMA
                     VLKLFSAHGYRTELCFPHWLGKIFGDNYVVDIIFSSGNGICKVDNAWFEHSVPGGILG
                     IAMKFCPAEELIWTKGFIMERERYDGADIAHLLRTRGAQLDWSRLLGRFDPHWQVLLS
                     HLILFDFIYPAERALVPRWVINDLLQRMQSESADHAPRARLCRGTLLSRSQYRIDVEN
                     CGYEDARQQPIGSMTPEQAMNWTKAGEEQLERKKIPAA"
     gene            complement(3273..3992)
                     /locus_tag="VFY96_15395"
     CDS             complement(3273..3992)
                     /locus_tag="VFY96_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5607908.1"
                     /translation="MRIAAIGDIHCNTTSHDLVRSLFAQIAQRADILLLCGDLVDYGL
                     PEEAAVLAKEISSLKIPTLAVLGNHEYESGKEDEVQRILTGAGATVLDGDARELMGVG
                     FAGVKGFGGGFGACALGPWGEEMTKQFVHEAVSEALKLECALARLRADKRIVLLHYAP
                     IQATVVGEPAEIFPFLGSSRLEEPLDRYSVTAVFHGHAHRGSPEGRTKADVPVYNVAM
                     KLLQNTFPDQPGFRILELPDC"
     gene            complement(4059..4445)
                     /locus_tag="VFY96_15400"
     CDS             complement(4059..4445)
                     /locus_tag="VFY96_15400"
                     /inference="COORDINATES: protein motif:HMM:NF025140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4168 domain-containing protein"
                     /protein_id="HEX5607909.1"
                     /translation="MRSNRVARKLSLGVLIAMLCGAGVATAVEVPAKPADGSGLSDKE
                     LKAFVKAYVDYQKIRRTYAPALEQTKDPARKKEIEQEANAKVKRSLDAQGLSVARYNQ
                     IFGQVNSNPPLRKKVLQQVEEERKKS"
     gene            complement(4997..>5409)
                     /locus_tag="VFY96_15405"
     CDS             complement(4997..>5409)
                     /locus_tag="VFY96_15405"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX5607910.1"
                     /translation="DPLLVVAIIQVESRFNHRAVSSAGAQGLMQVQPNVVTALVERGK
                     MLPTGKNINDPRVNVEVGVSYLAYLKEVFGDWKIALTAYNAGPSSVAKKIAAKEKLSF
                     DYAHKVLSAQRELRQQLASVANGASGSVEGKITG"
BASE COUNT         1194 a         1524 c         1457 g         1234 t
ORIGIN      
        1 cgccaggcca cctcgggtgt ttccgactcg gcgaggacga gcgttacggc atgctcggtc
       61 gcgagccgtc gtgcagattg tttacgatga ctgacatcgc ggttgcattc cagtaccagt
      121 cggctgccgg cgcggtcctc aatcaatttg aatcggctct cgacctcgat gcgccgcccg
      181 tgcttcgtca cttgcttcga ttcgctgctc cactctccac gtctcaccaa ctcggcttgg
      241 aattgcgcac gtccgttggg aaattccgtt tgcaatagtt cgtgactcga acgaccgaga
      301 gcttcgtgtt gcgcatagcc gtagagttgt tctgcgccgt ggttccagta gataatgggc
      361 ccgcgcagct cccaggccag aatcgcttca ttggcaaagt tcagaagatc tgcctggcgc
      421 cgtaaaaatt gctcgtgctg ccggagcgca atctccacgc ggtcgcgctc cttttcacga
      481 tccgcgagaa gttcgccggc gcgtgccatc tccacagcga gtgagtccaa ttccgcaacc
      541 ggtagcttgc cggtgagcgc cgccgcctca cctcgtccca agcgatgagc cgcggcagcg
      601 agctcgccga tcgaataggc cacccgccgc gcaaaaatca atgccgcagc gaggccaatc
      661 agcatcagca acaaaccacc ggcgccgaga gttgccagtg agcggtatag gatcgcgttc
      721 acttcagcgc tcggcaccgt aagcgccacg aaccagttcg acacgtgcga gcggctaatc
      781 gccgcataag cccggatctg gtccgcggtg attccgctga gaaattgttc gctcctctca
      841 atccccgttt gctgcagtaa cgagcccacc ggtctgccga ggtatttggc cgcgtcccgg
      901 gttcgcgcaa tggtaatgta ctggctgtcg taaaccgtcc cgacccaaga cggcggcaat
      961 ttttgctgca gtaaaatatc tgtaaatacc tgcggctcga tcgccgcggt cagaatataa
     1021 atgatcgttt gttcgcgcac caccggcaca tgaatattga cgccgctctc aggcgaatcg
     1081 acctgcggga aatcgctgat gaaaggacgg cgcgtccgca aaacggcatc gagactttcc
     1141 cggctgatgc ctttcggtgt gtcgaccagc ggctttgtca tttgagccag ctgccgacct
     1201 tggggatcga acagtgagat gcttttccag cttttttgcg tacgcaagat acgcgcagcg
     1261 acactacgaa aaacattgac ggcgccgaca tcgagggatt cagaggtcgc caatgcccgc
     1321 agattggtaa tcgatgattc tatttcgcga tcgatggcga gcgccaaagc gcgggcggtg
     1381 tcttccaagc ctctctgacg attcgcctgc tcctgacgcg cgaatagcac catcatgaaa
     1441 acggaaaaaa ccaggatcgg caccaggccg acaaagatca agcgaaccaa atacgcgttg
     1501 agcgccgtcg gttcactgaa ccaacgggaa acttgagggg gcaatcgaag catttcggat
     1561 caatggccgc cggtggctcg attgtggatc gaggccggca actgcgtcgt tcgcggcttt
     1621 tgcggattga aaagcactcg tttaattcgg cggttttcgg aggcaactag acgagcaacc
     1681 gcaacgcctc ggtcaattct tgcaaatcga ccggtttcgt caagtagcgc atcgctccct
     1741 ttgaaaatgc ttgtttgagc gaagcgaccg tcggatacgc cgtcatcatg atgactttca
     1801 gtcgtggcga gcgggcggtc acttgttcca ataacgtcaa accatcaccg ctgccgagtt
     1861 tgatgtccag cagagcgacg tcgaaactct gcgtctgcac catgtcgagc gcctcatcgg
     1921 cgctcagcgc ggtcgagacg tcataaccgc gctgagtcaa aaaccacttg agaagggtga
     1981 gaaaattttc ttcatcgtcg accacaagca ccgactgacg gatcgtatcc accgccttgg
     2041 gcaaactttc gctggtcccg tcttggttcg agcgtgcgga tttttcattc tccataggtc
     2101 gccactcgat cgacagagag ctcttgcggg gtgttgttta gcatcggaaa taatctggac
     2161 aaaacgcctc aaacaagctt tcgaaggaag ttctggatgc ggagaagtcc gtgcaatgct
     2221 tcctgataag taaccgagca gaatctatac caactgggct ccggtcccaa agcgctctaa
     2281 tgctaatcga ttgtcaatct cggtgaaaac gagagggcag acttgaaggg atttcgccga
     2341 caagagagac gattttcact tactttaagg atggaaaacg gaagagatta gcgaggttct
     2401 aaatcgtcgg aggcaagggg cttagcgcgt ctacgcagcg ggaatttttt ttcgctcgag
     2461 ttgctcctct cccgccttcg tccaattcat cgcctgttcc ggagtcatag agccgatagg
     2521 ctgctgccga gcatcttcgt aaccgcagtt ttcgacgtcg atgcgatact gagatctcga
     2581 cagcagtgtg cctcgacaga ggcgagcgcg cggtgcatgg tcggcgcttt cgctctgcat
     2641 ccgctgcagg aggtcgttta tgacccaacg cggcaccagc gctcgttctg ccgggtaaat
     2701 gaaatcgaaa aggatcaagt ggctgagaag gacttgccaa tgtggatcaa agcgtccgag
     2761 caatctcgac cagtctagtt gcgcgccccg cgtccggaga agatgcgcaa tgtccgcacc
     2821 atcataacgc tcccgttcca taatgaaccc cttggtccag attaactctt cagccgggca
     2881 aaacttcatc gctatgccga gaattccccc cggcaccgaa tgctcgaacc aagcgttgtc
     2941 gactttgcag atcccatttc ccgagctgaa aatgatatcg acgacataat tgtcgccgaa
     3001 aattttcccg agccaatgag gaaaacacaa ttcggtgcga tagccatgag cgctgaagag
     3061 cttcagcacc gccatcgcat gagcaggttc cagcaccaga tccaaatctt ttgagttgcg
     3121 cacaatcccg gtgtagtggt caagagcgta cccgccgccg acgagatatg gaatttgaga
     3181 gtcgttgatc aaattcagca catctctata aaaggcatgt tggccatttg cggctgaggt
     3241 tggggcgcaa gcttcttttt gcataatacg atttagcagt cgggaagctc gagtatcctg
     3301 aagcccggct ggtccggaaa ggtattttgc aaaagtttca tcgcgacgtt gtacaccggc
     3361 acgtcggcct tggtgcgtcc ctcgggagag ccgcgatggg cgtgcccatg aaacaccgct
     3421 gtgaccgagt agcgatcgag cggctcttca agtctgctcg agcccaggaa cggaaaaatt
     3481 tctgccggct cgccgacaac cgtcgcctga atcggtgcat agtgtaggag cacgattcgc
     3541 ttgtcggcgc gcaaccgtgc aagggcacat tcgagcttga gcgcctctga aaccgcttcg
     3601 tggacaaact gtttggtcat ctcctcgccc caaggcccga gcgcgcaagc accgaagcca
     3661 ccaccgaacc ccttgacgcc ggcaaatccg acgcccatta gttcacgcgc atcgccatcg
     3721 agtaccgtcg caccggcacc cgttaagatt cgttgcactt catcttcctt gccggactcg
     3781 tactcgtgat tgccaagcac tgcgagtgtc ggaattttta ggctggaaat ctccttggcc
     3841 aagaccgctg cttcttcggg cagcccgtaa tcgaccagat cgccgcatag aagaaggata
     3901 tccgcgcgct gagcaatctg tgcaaatagc gatcggacca gatcgtggga tgtggtattg
     3961 cagtggatgt ctccgatggc agcgatgcgc atggtttctt tttcaccgcg gccgagattc
     4021 ttgcgtatcg agaatttcgc gccaaagctt gagcatcgtc acgatttttt tcgttcttct
     4081 tccacttgtt ggagcacttt cttgcgcagc ggcggattgc tgttgacctg gccgaaaatc
     4141 tgattgtagc gcgccactga caatccttgg gcgtcgagag agcgcttgac cttggcgttg
     4201 gcctcctgct cgatctcctt cttgcgcgcc ggatctttgg tctgctcgag cgcgggagcg
     4261 taggttcgac gaattttctg ataatcgaca taagctttga caaaagcttt taattctttg
     4321 tcgctcaagc cgctgccatc ggctggcttc gcgggtactt ccacggccgt cgccactcct
     4381 gcgccgcaaa gcatcgcgat cagaacgcct agtgacagtt tgcgcgcgac tcgattcgat
     4441 ctcatcgtca caatctcctt tcgactttgg ttgcgtgcgc ttgcaatcta atttcctgcg
     4501 gcgctgtttg ctgcgtgtgt caggccgttc gagcgcccat gggatgtggc gatcccgaat
     4561 cgctccatag cagacaagct gatgttgtct cagtgccagg tttgagaaat gggcgccgaa
     4621 tgacttaaaa tattcagcag aacataagcg acgaaattat gtgcttgcct gcggttctgt
     4681 attaaacgat ccgcttcctc tgtcaacgcg acaatgagat ccgccaaagt ggcttccaag
     4741 gggtcgtctt tttcttgaaa catagcgcac ctctggttcc gcccgaattc gaggccagcc
     4801 aacagcgacg caatgcatgt gccacgaggc tgatttgact aggtcggcct taagaatcga
     4861 agtgacagag caaacatccc ggcatgcgcg caacacgaca atcctgttat cttgaacgaa
     4921 gtgagagaga attaaggaat tacagatggc ggctcaatcg ccagggagcg gcactgatcg
     4981 gacggctgcg gcgagcttag ccggttattt tgccttcgac acttccggag gcaccgttgg
     5041 cgactgaggc aagctgctgt cgcaactcac gctgggcgga aagaacttta tgcgcatagt
     5101 cgaaagagag tttttccttt gcggcaatct tcttggctac cgaggagggt cccgcattgt
     5161 acgccgtcaa tgctattttc cagtctccga acacttcttt cagatacgca agatacgaaa
     5221 caccgacttc gacgtttact cgtggatcgt tgatattttt cccagtcggc agcatctttc
     5281 ctcgttccac caacgctgtc acaacattcg gttgaacctg catcagacct tgcgcgccag
     5341 cggaggagac cgctctatga ttgaaccggc tttcaacctg aattatagcc actaccagga
     5401 gcggatcga
//
LOCUS       DASULZ010000749         3253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_898187, whole genome shotgun sequence.
ACCESSION   DASULZ010000749 DASULZ010000000
VERSION     DASULZ010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898187"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VFY96_15410"
     CDS             <1..477
                     /locus_tag="VFY96_15410"
                     /inference="COORDINATES: protein motif:HMM:NF025873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX5607911.1"
                     /translation="EYVCRALAPAKVSKILIDDEDHTMEVIVPDDQLSLAIGKKGQNV
                     RLASRLTGWRIDVRSEAEAEEETRRARQSISAIPGIGDFASELLYQEGFKSAEDIADS
                     DLAEILDVEGISKEKAEALHNSARQYVAEKRAREAEAAAAAAANVTAGEPAEPQSE"
     gene            753..>3253
                     /gene="infB"
                     /locus_tag="VFY96_15415"
     CDS             753..>3253
                     /gene="infB"
                     /locus_tag="VFY96_15415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX5607912.1"
                     /translation="MGKTRVHLLAKELGIETKDLIAQLDRMGLRGRKAQSALEEDEVA
                     RVRSALAAQERPQVHVGEEKVVADRMVKTEDETHRSAEARETVVERRVRANVIRRRVN
                     RTEIAPSEAPPPAPEPVAPPHVEASPEPEPAVPSIEEIPIDEFPQVEADEGISAAPET
                     PTKEPSVAPEKAPPSGAEPIFRPEPEKPQVNGQTQQPARQEAPRGARILGRIDLKQTM
                     RIEPAPTPVRRPPGAVPGQPGARVAEGEKPQAGAGETTATDDKPKAGRHKRRVVKKQD
                     VLELREKELRGGGRIPKKRRALPGKEIRKTEITVPRASKRVVKISEVITVADLSREMG
                     VKAGEVIKKLMAMGMMATINQVLDADTATLIASEFDHQVENVAFDVDTALELEHQVEE
                     ETQLVSRPPVVTIMGHVDHGKTSLLDAIRSTNVTAQEHGGITQHIGAYHVQVDGRSVT
                     FLDTPGHEAFTAMRARGAKVTDIVVLVVAADDGVMPQTVEAINHARAAEVPIIVAVNK
                     MDRPDANIEKVKRGLAEHGLASEDWGGDTVFAPVSAKTHEGIPHLLEMLLLQADILEL
                     TANPEKLARGTIVEAKLDRGRGPVATVLVQEGTLHGGDAFVCGVYYGKVRAMIDDHGR
                     KVESAPPSFPVEILGLQGVPQAGDSFVSVADEAKARQVAEYRQSKQRETELVKSSKVS
                     LEELYDQIKTGDVKELRVVLKADVQGSVEALTDALTRMSTDEVKLRAIHGSVGGITES
                     DILLAAASNAIVIGFNVRPESKGAALAAKEGVDVRLYTIIYEAVADVRAAMEGMLEPT
                     YREQTQARVDVRQIFNIAGVGTIAGCYVTEGKIARG"
BASE COUNT          756 a          873 c         1061 g          563 t
ORIGIN      
        1 gaatacgtct gccgggcgct cgcgccggct aaggtttcga aaattctgat cgatgacgag
       61 gatcatacga tggaagtgat cgtgcccgat gatcagctct cgctagcgat cggtaaaaag
      121 gggcagaacg tgcgccttgc ctctcggctg acgggatggc gcatcgatgt tcggagcgag
      181 gcagaagccg aggaggaaac acgccgggcg cgtcagtcaa tcagcgctat tccggggatc
      241 ggcgattttg cgtctgagtt gctttaccag gagggtttca agtcggctga agatatcgcc
      301 gacagcgacc tggcggagat tcttgacgtc gaggggatca gcaaagaaaa ggccgaggca
      361 ttgcacaact cggcgcggca gtatgtggcc gaaaagcgag cccgcgaagc agaagcagca
      421 gcggctgccg cagcaaacgt tacagcaggg gagcccgccg agccgcaatc cgaatagatg
      481 gtcaaaaagc accatcggcc gcagcgtacc tgtatcggct gcgggatgcg cgaccttcaa
      541 gagcggttga tccgtatagc tttggctgat cagaatagtt tggttcttga cgcaaatcgg
      601 gggcgcggcg gttatttaca ccgagacgaa aattgttggc gggctttcat cgctcggaaa
      661 agtcattatc gtgcttttcg cgctgaggtg agtcgcgcag ccaaggaaca attgattctg
      721 gaattgcagt gtcaaaatcg ggagtgagag aaatgggcaa aacgagagtt catctgttgg
      781 ccaaagaatt ggggattgag acaaaggatc tcatcgcgca gttggatcgt atgggtttac
      841 gcgggcgaaa ggctcaaagt gcccttgaag aggacgaagt cgcccgggtg cggagcgcgt
      901 tggccgctca ggagcggccc caggtccatg tcggcgagga aaaggtcgtt gccgatcgaa
      961 tggtaaaaac cgaggacgaa acgcaccgca gcgccgaggc gcgcgagact gtcgtcgagc
     1021 gccgtgttcg cgcaaatgtc attcgtcggc gtgtgaaccg aaccgaaatt gctcctagcg
     1081 aagctccgcc gcctgcgccg gagccggtcg cgccgcccca tgtcgaagct tcgccggaac
     1141 ctgagccggc ggttccgagc atcgaagaaa ttcccattga cgaatttccg caagtcgaag
     1201 ccgatgaggg aattagcgca gcgccagaaa cgccgactaa agagccatcg gttgcgccgg
     1261 agaaggcgcc gcccagtggc gcggagccga tttttcggcc agagccggag aagccgcaag
     1321 tcaacgggca aactcagcaa cccgcgcggc aagaggcacc gcgcggcgcg cgcattctgg
     1381 gccgcatcga tctcaagcaa acgatgcgta tcgaaccggc acccactccg gtgcgccgtc
     1441 cccccggcgc tgttcccgga cagccggggg cgcgcgtcgc ggagggcgaa aagccccaag
     1501 ccggtgcggg cgagacaacc gccaccgacg acaaaccgaa ggccgggcgg cataaacgca
     1561 gggtcgtcaa gaagcaggat gtgctggagt tacgagaaaa ggagctgcgc ggcggcggtc
     1621 gtatcccgaa aaaaaggcgc gcgttgccgg ggaaagaaat cagaaagacc gaaatcaccg
     1681 tgccgcgcgc gagcaaaagg gtggtgaaga tatcggaagt gatcacggtg gccgatctgt
     1741 cgcgagaaat gggcgttaaa gccggggagg tcatcaagaa attgatggcg atgggcatga
     1801 tggcgacgat taaccaggtg ctcgacgccg acactgcgac tctgatcgcc tccgagtttg
     1861 atcatcaggt ggaaaacgtc gctttcgacg tggatacggc tctcgagctc gagcatcaag
     1921 ttgaagagga aacgcaattg gtgtcccgtc ctcccgtggt gacgattatg ggacacgtcg
     1981 atcacggcaa aacgtcattg ctcgacgcaa ttcgaagcac caacgtgaca gcacaggaac
     2041 acggcggcat cacccaacac atcggcgcct atcacgttca agtagacggc cggagcgtaa
     2101 ccttcttgga tacgcctggc catgaggctt ttaccgcgat gcgggcgcgc ggcgcgaaag
     2161 tgaccgacat cgtcgtcttg gtcgtagcgg cagacgatgg agtcatgccc cagacggtgg
     2221 aagcgatcaa ccacgcgcgc gccgccgaag tgccgatcat cgtcgcggtg aacaagatgg
     2281 accgaccgga tgctaatatc gaaaaagtga agcgcgggct tgcggaacac ggtctggctt
     2341 cggaagactg gggcggcgat acggtgttcg ctccggtatc ggcaaaaacg cacgaaggca
     2401 ttccacatct gctggaaatg ctcttactgc aagccgatat tctggagctc acagccaatc
     2461 ccgagaaact agcgcgcggc accatcgtcg aagccaagct cgaccgcgga cgcggtccgg
     2521 tggccactgt tctggttcaa gaaggtactt tgcacggcgg cgacgccttt gtttgcggcg
     2581 tgtactacgg caaagtgcgc gccatgatcg atgaccatgg ccgaaaggtt gagtctgcgc
     2641 cgccatcatt cccggtcgag attctcggcc tccagggcgt gccgcaggcg ggtgattcgt
     2701 tcgtgtcagt cgccgatgaa gctaaagccc gtcaagtcgc cgagtatcgg caaagcaagc
     2761 agcgcgaaac cgagctggtg aaatcgagca aggtttcgct ggaagaattg tacgatcaaa
     2821 ttaaaaccgg cgatgtgaaa gagctccggg tcgtgctcaa ggcggacgtg cagggctcag
     2881 tggaagcgct caccgatgct ttgacccgca tgtcgacgga cgaggtcaaa ttgagagcga
     2941 tccacggctc ggtcggggga attaccgaga gcgatatttt gctcgccgct gcatcgaacg
     3001 cgattgtcat cggctttaat gtccgtcccg aatcgaaggg cgcggcgttg gcggccaaag
     3061 agggcgtcga cgtgaggctc tacacgatca tctacgaggc tgttgccgac gtgcgcgcgg
     3121 cgatggaagg catgctcgag ccgacctatc gagagcagac gcaggcgcgc gtggatgtgc
     3181 gtcagatctt caatattgcc ggggtcggga cgatcgccgg ttgctatgtc accgaaggca
     3241 aaatcgcgcg cgg
//
LOCUS       DASULZ010000750         4176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1506484, whole genome shotgun sequence.
ACCESSION   DASULZ010000750 DASULZ010000000
VERSION     DASULZ010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506484"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..1347)
                     /locus_tag="VFY96_15420"
     CDS             complement(217..1347)
                     /locus_tag="VFY96_15420"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5607913.1"
                     /translation="MELKHGVVSADDHVQEHPEVWTSRMSRAKWGDRIPHLARMDDGS
                     EEWMIEGWRIPLYGVALANAAMDDRAQEPKRWNEVPRAVFSAAERLKAMDIDGIDYSV
                     LYPSVSGFAGQIFGGVVDPDLEIACVQAYNDWLIEEWAGVSPRFVPQCIVPIWPMDWT
                     VAEINRAVKKGHKGVIYPASPMELRRVPHINDPAYDPLWATCQDLGVPLCFHAGGSAK
                     IQMLPGSAFKPAVAAAFSDVVRSVSSIAVVANFLLARVLDRFPRLKVVFAESSIGWGA
                     YELEYADYQSAADGLPSEGYDLKPSELFQRQCYFTCWYDRMSFRARRHLGTGNILWSS
                     HFPLASSTWPDTRRHLESALAGVPADEKRQIQWENAAKLYRL"
     gene            complement(1349..2506)
                     /locus_tag="VFY96_15425"
     CDS             complement(1349..2506)
                     /locus_tag="VFY96_15425"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5607914.1"
                     /translation="MEIKYGLISSDSHGQLGRDAYTSRMSKDRWGERIPHVIEVRDDK
                     YTEPVERWIVNGEVQGGNVCNCPTAMPNRSYYPQRWEEVPPEVYQPTERVKALDRDRI
                     DAEVLFPNGPGGTFFYDDTEFELACVRAYNDALAEFRQTSERFIPLAVIPFMSPIETI
                     IGEVERAVKAGHLGVNMLADPSVAVHGLKHLGDPFWEPLWAACEELGVGVHLHASAGL
                     AGKLSLPRWQGFSTRQAHCVSTLRNFCTATQFVPYLIFSGMLEKFPRLNWVFAETGLG
                     WVSSILEACDSEWEKRRLWKDGIVTRPSDLFRRQIYVDFWFETVGIELRDCIGVDNIL
                     WESDFPHITSTYPNSWNAVEHSLAGVPDAEREKMLYRNAARLYRVDLVFAS"
     gene            complement(2772..3470)
                     /locus_tag="VFY96_15430"
     CDS             complement(2772..3470)
                     /locus_tag="VFY96_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607915.1"
                     /translation="MSLWPKVREELNTMVNRHFETPEYRQLFSVKLTPARQKIWDIHY
                     PHYIKSRRDCWGAAAVHAPLDVKRAIWEHEKDELIFDKRMGSAHLTDEQMAVAAAEAS
                     LLPGVRAALMAWMFLSTTRPWIEALTVNHITERKNNPAIVKGGGFTARFAHKRVADQG
                     GTIEALDINTKVHMVADEDHSDMFEPIYDRHIIDERTAEAVIRAAQDSLAVDRAYRSA
                     LATAMAQIAESAAA"
     gene            complement(3604..>4176)
                     /locus_tag="VFY96_15435"
     CDS             complement(3604..>4176)
                     /locus_tag="VFY96_15435"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX5607916.1"
                     /translation="VRHRRECWSHVSANCPIMGVKQRILEHEYEEIIRDDFSDYGHLH
                     LIIRQGKSVGLEPEDIIEAEPLADTRAVLYAWSWMTSEMPWTESLTAMTITEWCNDDR
                     LLGDLGGGQSTRMAKKWMEDMGFSWKEIPNLQAHSQADEKHSDMFLPFLADYASGSKE
                     APAIQAAKDSLAFNAMYRKGIALAQEKIPL"
BASE COUNT          928 a         1242 c         1072 g          934 t
ORIGIN      
        1 aaacctcctt tcaatcgtta actgctcaaa gcaaatccga aatccgaata tccaaatccg
       61 aaaaaaaacg acaaaccaaa aaccatcgat tgaataaatt ctcaaactcg agcgatcgat
      121 ttaacccttg aattccgaac ttttcggtcg tttgaattct gttgcgaatt tcggatttcg
      181 agctttaaat ttcgtcgtgg cgccgctcga gaattattat agtcgatata actttgccgc
      241 gttctcccat tgaatctgtc ttttctcgtc ggccggcacg cccgctaagg cactttccag
      301 gtgccggcgt gtgtccggcc acgtcgaact cgcgagggga aaatggcttg accataatat
      361 gttcccagtg ccgagatggc ggcgtgcgcg aaaactcatc cgatcatacc agcaggtgaa
      421 ataacactgc cgctgaaaca attccgatgg cttcaaatca taaccttcgg atggcaagcc
      481 atcggccgcc gattgatagt cggcgtactc aagttcatag gcgccccaac caatggagct
      541 ttccgcgaaa acgactttca gccgcgggaa gcgatcgagc acgcgggcga gcagaaaatt
      601 cgccaccacc gcgatcgagc tgaccgaacg aaccacatcg ctgaaggccg ctgcgaccgc
      661 aggcttgaat gcgcttcccg gcaacatctg aatcttcgcc gagccgccgg catgaaaaca
      721 aagcggcaca cccaggtctt ggcacgttgc ccagagtgga tcataagccg ggtcgttaat
      781 atgcggcacg cgccgaagct ccattggcga cgctggataa ataacccctt tgtggccttt
      841 ttttacggcg cggttaattt cggcaacggt ccaatccatg ggccagatcg ggacgatgca
      901 ttgaggcaca aagcgcgggc tcactcccgc ccattcttcg atcaaccagt cgttgtaagc
      961 ctgcacgcag gcgatttcca aatccgggtc gaccacaccg ccaaaaattt ggccggcaaa
     1021 tccgctgaca ctgggatata gaacagagta gtcgatgcca tcgatgtcca tcgcttttag
     1081 ccgttccgcg gcgctaaaaa ccgcgcgcgg aacttcgttc cagcgcttcg gctcttgggc
     1141 acgatcgtcc atggccgcgt tcgccagagc gacgccatat aacggaatgc gccagccttc
     1201 gatcatccac tcctcgctgc catcgtccat gcgcgcgaga tgcggaattc tgtcgcccca
     1261 ctttgcgcgc gacatgcggg acgtccagac ctccggatgc tcctgcacgt gatcatccgc
     1321 gctgactacg ccgtgtttaa gttccatttt agcttgcgaa cactaaatca acccggtaca
     1381 gtctcgccgc attgcggtaa agcatttttt cgcgctcggc atcgggtaca ccggccaatg
     1441 aatgctcgac ggcattccag gaatttggat aggtggaagt aatgtgcggg aagtccgatt
     1501 cccaaaggat attgtcgact ccgatacagt cgcgcaattc aataccgacg gtttcgaacc
     1561 agaaatcgac gtagatctga cgccggaata agtcgctcgg ccgtgtaaca atcccgtcct
     1621 tccaaagacg gcgtttctcc cactcagaat cgcaagcctc aaggatcgaa ctcacccatc
     1681 cgagcccggt ttcggcgaag acccagttca gccgcggaaa tttttccagc atgccggaaa
     1741 aaattaaata gggaacgaat tgcgtggccg tgcagaagtt gcgcaatgtc gagacgcaat
     1801 gcgcctgacg cgtgctgaag ccttgccatc gcggcaacga aagcttgcct gcaagccccg
     1861 cggatgcgtg gagatgaacg ccgacgccaa gctcttcgca agccgcccag agcggctccc
     1921 aaaaaggatc accgagatgc ttcaatccgt gcaccgccac actcgggtcg gctaacatgt
     1981 tgacgccaag atgacccgct ttgaccgcgc gctccacctc gccgatgatt gtctcgatcg
     2041 gactcatgaa tggaatcacc gcgagcggaa tgaaccgttc gctggtctga cgaaactcgg
     2101 ctaacgcgtc gttgtaagcg cgcacgcagg caagctcgaa ctcggtatcg tcgtaaaaaa
     2161 aagttccgcc cggtccgttg gggaacaaca cttcggcatc gatgcgatcc cgatccaagg
     2221 ctttgacgcg ctctgtcggt tgatagactt ccggcggcac ttcctcccag cgctgcggat
     2281 agtagctgcg gttgggcatt gctgtggggc agttgcaaac attgccgcct tggacctcgc
     2341 cgttgacgat ccatcgctcc actggctcag tgtatttgtc gtctcgcact tcaatcacgt
     2401 gcggaatgcg ctcgccccac ctgtctttcg acatccggct cgtgtacgcg tccctgccca
     2461 gctgcccgtg cgaatccgag ctaatcaagc cgtatttgat ttccatgatg ttgtctttgt
     2521 gtgaggagac gttaactaga ggcgctggct actcccgctc ccggtgaatc gagcaaatta
     2581 ggcatggttc cactcgacgg ggagaataga agaggggtga tgaagcgaca gccctcgacc
     2641 cgctcacccg tatctcagtc ttcccccatc aaagggggca gaggcgacga acatacaaaa
     2701 tgaccggcct tagtgacttt gcgcgatgtc ttgaactttg aacacggaac cttgaacctc
     2761 ggatacgcga cttacgccgc cgcggactcg gcaatttgcg ccatcgccgt ggcgagagcg
     2821 cttcgataag ctcgatcgac cgcgaggctg tcctgcgcgg cgcggatcac cgcctccgcc
     2881 gtccgctcat caataatatg acggtcatag atcggctcga acatgtccga atgatcctcg
     2941 tccgccacca tatgcacttt ggtgttgatg tcgagggctt cgatggtacc gccctgatca
     3001 gcgacccgct tgtgggcgaa acgcgcggta aacccgccgc cttttacaat cgccggattg
     3061 ttcttgcgct cggtaatatg atttaccgtc aaggcttcga tccaggggcg cgttgtcgag
     3121 agaaacatcc acgccatgag cgcggcgcgg actcccggca gcaaactcgc ttctgccgct
     3181 gccaccgcca tctgctcgtc ggtgagatgc gccgatccca tacgcttgtc gaaaatcagt
     3241 tcatctttct cgtgttccca aattgcccgt ttcacgtcga gtggcgcgtg cacggccgcg
     3301 gcaccccaac agtcgcgccg gctcttaatg tagtgcggat agtggatgtc ccaaatcttc
     3361 tgacgggccg gcgtcagctt tacggaaaaa agctgacgat attccggcgt ttcaaaatga
     3421 cgattcacca tcgtgttgag ctcttctcga acttttggcc aaagactcat aaatacctcc
     3481 tgatgtgcct acgatctcat cgagtcagct ttcgctacgg gcaagacttc tccctgtcgc
     3541 ctcgaaatga cagatcacga gtacttcttc agtcccgagt aacagataat ttcttgctat
     3601 gttctacagc ggaatctttt cttgtgccag agcgatgccc ttgcgataca tcgcattgaa
     3661 tgccaacgag tctttcgccg cttgaatcgc cggcgcttcc ttgctccctg aggcgtagtc
     3721 ggcgagaaag ggcaaaaaca tatcgctatg tttttcgtcc gcttggctgt gcgcctggag
     3781 attgggaatt tctttccagc taaaccccat gtcttccatc catttttttg ccatccgcgt
     3841 ggactggccg ccgcccaggt cgccgagcag ccggtcgtcg ttgcaccact cggtaatcgt
     3901 cattgcggtt aacgattcgg tccatggcat ctcgctagtc atccatgacc aggcataaag
     3961 cacggcgcgg gtgtcggcca gtggctcggc ttcaatgatg tcctccggct cgagtcccac
     4021 ggacttcccc tgtcgaatga tcagatgcaa atgtccatag tcggaaaaat cgtcgcgaat
     4081 gatctcttcg tattcgtgct ccaaaatccg ctgcttgaca cccatgatcg ggcaatttgc
     4141 cgaaacgtgc gaccaacact cacggcgatg gcgcac
//
LOCUS       DASULZ010000751         5421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_695164, whole genome shotgun sequence.
ACCESSION   DASULZ010000751 DASULZ010000000
VERSION     DASULZ010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695164"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VFY96_15440"
     CDS             complement(<1..395)
                     /locus_tag="VFY96_15440"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX5607917.1"
                     /translation="MSARQLTSHIRERKISAREVMSAFLRQIARLNPKINAIVAKLDD
                     EQCLALADDADRRVARGEEVGPLHGVPFAFKDLDPAVGFPFTRGSSIFRDFMPTEDSV
                     LVERSRRAGVIAIGKTNVSEFGMGSHTYN"
     gene            complement(581..1039)
                     /locus_tag="VFY96_15445"
     CDS             complement(581..1039)
                     /locus_tag="VFY96_15445"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX5607918.1"
                     /translation="MDSGFHASFALLRHRIAGDCDASPKGGMPGFVKVLNEKQLFIPD
                     VAGNKLFHGYGNIESNPKVGLIFLIPGHNSTVRINGRVKVIDRSDFTDRKLEVFDPDE
                     KAEVLQGLLVEVAESYSHCPRALKFSRLWNVDEISKNIADPPVGLKEPGI"
     gene            complement(991..1116)
                     /locus_tag="VFY96_15450"
     CDS             complement(991..1116)
                     /locus_tag="VFY96_15450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607919.1"
                     /translation="MSTKYQDKFGHPVERTKGKVRPFMAGWIQDFMRHSPFCVIA"
     gene            complement(1133..1834)
                     /locus_tag="VFY96_15455"
     CDS             complement(1133..1834)
                     /locus_tag="VFY96_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX5607920.1"
                     /translation="MAHQREAKVCGICGKTFALRDLVPGIALRDAVAQQISHEHPDWS
                     PEKFICRPDLNRYRGEYVHSLLESEKGELTSLENEVLTSLRDHETLSKNVDSEFEGEW
                     TLGERWADRIATFGGSWIFLILFALFLALWISINSFILYAHPLDPYPYILLNLILSCV
                     AAIQAPIIMMSQNRQEAKDRLRSKHDYQVNLKAELEIRHLHEKLDHLLSHQWERLAQI
                     QEIQVELLAEISRKR"
     gene            complement(1862..2452)
                     /gene="nbaC"
                     /locus_tag="VFY96_15460"
     CDS             complement(1862..2452)
                     /gene="nbaC"
                     /locus_tag="VFY96_15460"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HEX5607921.1"
                     /translation="MGKGEQHSNGNLMDFFSTFHLKQWVRENQALFDPPFRTNKLLVS
                     HKDFIVMMLRGPNTRLDFHIDPGDEFFYQVEGEMELVVKPEGERRQTVKIKEGEIFVC
                     PGSIPHSPRRFENTWGLVIERKRRPEEQEEFVWFCEQCDALVLSRVVNQGNIPAQVTA
                     VYTEFNNDTRLRTCKSCGYVFPETPMAERLSFLEKS"
     gene            complement(2421..3209)
                     /locus_tag="VFY96_15465"
     CDS             complement(2421..3209)
                     /locus_tag="VFY96_15465"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX5607922.1"
                     /translation="MSAEAVLRGETLSTKQFSDVLINEVKAGHARLHHPFYLDLYEGK
                     LPLEAVKIWAKEAWGIFSYNVAINTAKLVRCQLSGIHDPEIHKKFVDIIHSEVGYTYF
                     EGSPRPVLGHRALFLRFGESIGIPAKELERCEAEEDFLPTTVLARVGWLDIALRSNHI
                     LEQVAATNCCNEFSNQLTGGKFFRSFRDHYGLKPHDIEFFAEHGEADTEHSNIGYELV
                     EEFATTNELQVRVLKALRKGLSIWWALTDGVAREVGKRRATLQR"
     gene            complement(3329..3772)
                     /locus_tag="VFY96_15470"
     CDS             complement(3329..3772)
                     /locus_tag="VFY96_15470"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5607923.1"
                     /translation="MKIRNGVLGHIVLNVADLKKSEEFYRSILGLKVSARNLDTKMTF
                     MSYGKEHHDIALQALPKGVKHVDGDGAPKLHHFCVYVDSNEIIERIYPVLQRRKIPIV
                     SGPETLVVAGNRSISFLDPDGNRVEIACNANKFKHDNRIPRKLRS"
     gene            4116..4823
                     /locus_tag="VFY96_15475"
     CDS             4116..4823
                     /locus_tag="VFY96_15475"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX5607924.1"
                     /translation="MKIMIVMGEYPEEEAERRRQAVLKCASAGTEIGFAVIKATFFRR
                     SNSQVNSLSAGPLVAEVAQRAEADGYDAVVPFGTLDAGVELARNLIRIPVVGAGQSVL
                     HLGTQLSNRLGVIAYEEKSIPFMRKQMHAWRVADSIVGIRSVEIPLPESTKNRSAMRA
                     RFIQVACELIDNFDAEIIVPMGVTMVPVQYSPQEFASELGVPVMDALKTSIQTAEMLV
                     RMGLTHSVRTYPRPATN"
     gene            4952..>5421
                     /locus_tag="VFY96_15480"
     CDS             4952..>5421
                     /locus_tag="VFY96_15480"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5607925.1"
                     /translation="MSLVGTSVERVGIRAKVSGSLSYIGDLQFPGQIYAKALRSPYPH
                     AKLVRIDPSHAAAFPGVHAAVTREDLADLNPCFGTGVEDQPVVVIDRSRYAGDIVAAV
                     AAETREIAEEAAALVAVQYEPLAAATDLIEAARQGAPIIHEQHVDPSAGGNIHGV"
BASE COUNT         1257 a         1483 c         1414 g         1267 t
ORIGIN      
        1 tcgttgtacg tatgcgagcc catgccgaac tcagagacat tggtcttacc gatcgcgatt
       61 acgccggcgc gccgcgatcg ttcgaccaaa acggaatctt cggtcggcat gaagtcacga
      121 aagatcgacg atccccgggt aaacggaaaa ccgaccgcgg gatccaaatc tttaaatgcg
      181 aacggtactc cgtgcagcgg cccgacttct tcgccgcgcg ctacgcgccg atcggcatcg
      241 tcggcaagcg cgaggcactg ctcgtcgtct aatttagcaa cgattgcatt gattttcgga
      301 ttcagtcgcg cgatctgtcg cagaaatgcg ctcatcacct cgcgcgcgga aatttttcgc
      361 tcgcgaatgt gagacgtcag ttgtcgcgcg ctcatgaaac agatttcttg atccgacctg
      421 gaactcattt gctggtgacg acggttatcg gattatcacc aactgtttaa cggctgtgcg
      481 atgtcgagtg gctcagggta aactccggga gaaatctttc cccaaacaag agccaattga
      541 aaatcggaat cgtccgcaaa cagcgatctg cgaatcctga ctaaattccg ggttctttca
      601 ggcccacagg tggatcggcg atgttcttcg agatctcatc cacattccat agccgcgaaa
      661 atttcaatgc tctagggcaa tggctatagg actctgcgac ttcgacgagc aagccctgaa
      721 gcacttcggc tttttcgtcc ggatcgaaga cctcgagctt gcggtcggtg aaatcgcttc
      781 gatctatgac ttttaccctg ccgttgatcc gcacggtcga attgtgccct ggaatgagaa
      841 aaatcaagcc aacttttgga ttgctttcta tattgccata cccatgaaaa agcttattgc
      901 ctgcgacatc gggaataaac aactgctttt cgttcaaaac cttcacgaac ccaggcatgc
      961 cacccttcgg cgaagcgtcg cagtcgccgg ctatgcgatg acgcaaaagg gcgaatgacg
     1021 catgaaatcc tgaatccaac cggccatgaa aggtcgcact tttcctttgg ttcgctccac
     1081 tggatgaccg aatttatcct ggtatttggt gctcatcagc atctcctaat cttcagcgct
     1141 tccggctgat ctcagccagc agctcgacct ggatttcctg gatctgcgct agacgttccc
     1201 attggtgcga cagcaaatga tcgagcttct cgtgcaggtg gcgaatttct aactcggctt
     1261 tgagattaac ctgatagtcg tgtttcgaac gtagccgatc tttggcttcc tgccggtttt
     1321 ggctcatcat aatgatcggc gcttgaatcg ccgctacgca ggaaaggatc aggttcaaca
     1381 atatataggg atagggatcg agtggatgcg cgtataagat gaacgagttg atacttatcc
     1441 aaagcgcgag aaataacgcg aacaagatga gaaatatcca actgccgccg aatgtcgcaa
     1501 tgcgatcggc ccaacgctcg ccgagcgtcc attctccctc gaattcacta tcgacatttt
     1561 ttgaaagcgt ctcgtgatcg cgcaggctgg tgaggacttc gttttcgagt gacgtcaatt
     1621 cacccttttc cgattccaac agagaatgaa catattcgcc gcgataacgg ttcaaatccg
     1681 gacggcaaat gaatttttcc ggtgaccagt ccggatgttc gtgcgaaatt tgctgagcta
     1741 cagcatcccg cagcgctatt ccggggacca aatcgcggag cgcgaacgtc ttgccgcaaa
     1801 tcccacagac tttggcctct cgctgatgtg ccatgcgctt caggatccag atatttgagg
     1861 actagctctt ctccagaaag ctcagccgct cggccatagg ggtttctgga aagacataac
     1921 cgcaggactt gcaggtacga aggcgtgtat cgttgttgaa ttccgtgtag acggcagtca
     1981 cttgtgccgg gatattgccc tgattgacga cgcgagaaag aaccagcgcg tcgcactgtt
     2041 cgcagaacca aacaaactct tcctgttcct ccggtcgacg cttgcgctca atcaccaacc
     2101 cccaggtgtt ttcaaaccgc cgcggtgagt gcggtatgct gcccggacag acaaaaattt
     2161 cgccttcttt aatcttgacc gtctggcggc gctctccctc cggcttgacg acaagctcca
     2221 tttcaccctc gacctggtaa aaaaactcgt ccccgggatc gatatggaaa tcgagccgcg
     2281 tattcggccc gcgcagcatc ataacaataa aatccttgtg gctgaccaac agcttgttgg
     2341 tacgaaacgg cgggtcaaag agggcctgat tttccctcac ccactgcttg aggtgaaatg
     2401 tggagaagaa atccatgagg ttaccgttgg agtgttgctc tccttttccc aacttcccgc
     2461 gccacaccgt cagtgagtgc ccaccagatc gataaacctt tgcgtaaagc cttcagcacc
     2521 cgtacctgca actcattcgt ggtggcgaat tcctcaacca gctcgtagcc gatattgctg
     2581 tgctccgtat cggcctcgcc gtgctcggca aaaaattcga tatcgtgcgg cttcaatccg
     2641 taatgatcgc ggaaactacg gaagaatttt ccgccggtca actgattgga gaattcgtta
     2701 cagcaattgg ttgccgctac ctgttcgaga atgtggttac ttctcagggc gatatcgagc
     2761 caacccactc ttgccaggac agtggtcggc agaaaatcct cctcggcctc acagcgctcg
     2821 agctcttttg ccggaatacc gatgctctcg ccgaaacgaa ggaacaaggc gcgatgaccc
     2881 aggacgggac gcggcgagcc ttcgaaataa gtgtatccca cttcagagtg gataatatcg
     2941 acgaatttct tgtgaatctc cggatcgtga atgccggaga gctggcagcg caccagcttc
     3001 gccgtattga tcgcgacgtt gtaggaaaag atgccccacg cttccttcgc ccaaatcttg
     3061 accgcctcca gcggcagctt gccttcatag agatccaggt agaacgggtg gtgcaaccgc
     3121 gcgtggccag ccttgacttc gttgatcaac acgtcggaaa actgttttgt cgatagcgtt
     3181 tcgcccctta aaaccgcttc ggctgacatt cgttcacctc gaattgttat tgcggcctca
     3241 acaccaccgt gtgccgcggt ttatgcagag gtatcacctc ggtaagccca ttcaacgttg
     3301 tcttagaccg atgctcgtcg tcaccattct agcttcgcag ctttcgcggg atgcgattat
     3361 cgtgtttgaa cttgttggcg ttacaggcga tctccacccg gttgccgtcc ggatcgagaa
     3421 agctgatcga ccgattaccc gcgacaacaa gagtttccgg accgctgaca atcggaatct
     3481 ttctgcgctg caaaaccgga tagatccttt cgatgatctc gttcgaatcc acatagacgc
     3541 agaaatgatg caatttaggc gcaccgtcgc cgtcgacgtg cttcactcct tttggcagtg
     3601 cctgtagcgc gatgtcatgg tgctctttgc cgtagctcat gaaggtcatc ttcgtatcga
     3661 gatttcgcgc cgaaactttc aaaccgagaa tcgagcgata aaactcttct gactttttga
     3721 gatccgcgac gttgagcacg atgtgtccaa gcacgccatt gcgaattttc ataggaggtc
     3781 ataataaata acggtactgg cttggtgtca aggcgatccg aaagcgacgg cttgagagaa
     3841 tctctttcac aagctgtgtc ctaacgctgt tagaatgatt tcagatgacc ggggcttgct
     3901 ctattgtcat ttgaagcggc ggatggtgtg aaaactagcg ttcgatacgg cagcgctact
     3961 cgctgccggc tcagtataat cggacggaat cgagttcccg agtagcgcga agcgcgtatc
     4021 gagggatacg cgacccgtag ggtcggagct ttgacaccgt ttgggcgaaa gcgagaagtt
     4081 tttaattatg cgctgcgtaa attcgaggtg aattcatgaa aattatgatc gtcatgggtg
     4141 aatacccaga agaagaggca gagcgccgta gacaggctgt gctgaaatgc gcctcggcgg
     4201 gcactgaaat cggctttgcc gtgatcaagg caactttttt tcgccgctcc aattcacagg
     4261 tcaattcgct ttctgccggg ccgctcgtcg ccgaagtagc acaacgagct gaagcggacg
     4321 gctacgatgc cgttgtcccg ttcggcacat tggatgccgg cgtcgagctg gcgagaaatc
     4381 ttatccgtat tcccgtggtt ggcgccggcc aatcggtttt gcacctcggc acgcaattga
     4441 gcaatcgttt gggggtgatt gcctacgaag aaaaaagcat cccgttcatg cgcaagcaga
     4501 tgcacgcctg gcgtgtcgcc gattccatcg tagggatccg cagcgtcgaa attccccttc
     4561 cagaaagcac caaaaatcgc agcgcaatgc gggcacgctt tattcaagtc gcgtgcgagc
     4621 tgatcgacaa cttcgatgcc gagatcatag tccccatggg agtgacgatg gtgcccgtgc
     4681 aatactcgcc tcaggaattc gcaagcgaac tcggcgtacc ggtgatggat gcgctcaaga
     4741 ccagcatcca gactgccgaa atgctcgtgc ggatgggatt aactcacagc gtcagaactt
     4801 acccgcgtcc ggctacgaat tgaacggccg ctggccgaaa ctgaagagat ccagacatgg
     4861 cgacgatata tatttccgct ccgtcattcc ggcaattccg gcatcgaggc cttgacgcgt
     4921 cgactcacct caaaggccat gatggcatag tatgagcctc gtcgggacta gcgttgaacg
     4981 agtcggcatc cgcgccaaag tcagcggcag ccttagctat attggcgatc tgcaatttcc
     5041 cgggcagatc tacgcgaaag cgctccgcag cccttacccg cacgccaaac tggttcgcat
     5101 cgacccaagc cacgccgctg cgtttcccgg cgttcatgca gccgtgacac gcgaagactt
     5161 ggcagatttg aatccgtgct tcggcactgg ggtcgaagat caacctgtcg tcgtgatcga
     5221 taggagccgc tatgcaggcg acattgtagc cgctgtggct gcggaaactc gggaaatcgc
     5281 ggaagaagcc gcagccctgg tcgcagttca atatgagcca ctcgcagcag cgacggattt
     5341 aattgaagct gcaagacaag gcgctccgat tattcatgag cagcatgtcg acccgagcgc
     5401 tggcgggaat attcacggcg t
//
LOCUS       DASULZ010000752         4282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1506828, whole genome shotgun sequence.
ACCESSION   DASULZ010000752 DASULZ010000000
VERSION     DASULZ010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506828"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VFY96_15485"
     CDS             complement(<1..345)
                     /locus_tag="VFY96_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607926.1"
                     /translation="MAFGRTILVCIVVLWTTFFGPATWALAPSYDIAVRELFPLGDWQ
                     KQFRITDGKDRGKLVPLSLHRAGGVQESWRLDFGDYAGIHLKNDVSGLTMDRLDLIKS
                     RSFIVYEPALPIF"
     gene            complement(458..619)
                     /locus_tag="VFY96_15490"
     CDS             complement(458..619)
                     /locus_tag="VFY96_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019942548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX5607927.1"
                     /translation="MKSVKPFLLLSSPLALTGCQVIGDIFKAGVWTGVVLVVAVVGVV
                     IWLVARSFS"
     gene            complement(667..957)
                     /locus_tag="VFY96_15495"
     CDS             complement(667..957)
                     /locus_tag="VFY96_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607928.1"
                     /translation="MKLKALDFWPPQEWKADTQTLAPEDALDATDVSASMPLNSSDVL
                     ALTVIHNGQTYAASVSVPENLAKRLVLVISGASPSKSLREIGELEIKSEVPQ"
     gene            1340..2389
                     /locus_tag="VFY96_15500"
     CDS             1340..2389
                     /locus_tag="VFY96_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX5607929.1"
                     /translation="MAASDSLPVDKISLPAGFQIKPYAQVPNARSMTLSPNGTLFVGN
                     RSGGKVYAIQTRDEGAQIKTPLVIAERLNMPNGVAVKDGALYVAEINRVLRYDDIEAR
                     VKNPPQPVVVNDRFPRDRQHGWKFIAFGPDGDLYVPVGAPCNICEPDADRYAALMRMK
                     PNGSNLEVFAHGIRNTVGFDWHPQTNELWFTDNGRDRLGDNVPPDELNHASKAGLHFG
                     YPYCHGGDIPDPEYGKKKPCSEFTPPAQTLGPHVAALGMRFYTGSMFPSEYLNQIFIA
                     EHGSWNRSTPIGYRVTVVELKGNKAVSYRPFAQGWLQGNRAWGRPVDVLVMPDGALLV
                     SDDEAGAIYRISYKK"
     gene            complement(2422..3738)
                     /locus_tag="VFY96_15505"
     CDS             complement(2422..3738)
                     /locus_tag="VFY96_15505"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5607930.1"
                     /translation="MAQIKEETAEQSGYQKLLEELPRTGPGTRAGEMLRRYWHPVCLS
                     ANLKDVPVPVRMLSEDLVLFRDGRGKPGLLGIRCPHRLASLEYGQVREDGLMCSYHGW
                     RFDTRGRCIDQPLEPRDSALKNNIRHVWYPIQEWGGVIWTYMGAEKENPPPLPKIDVL
                     ARTDGELKLVDGDVRNYNYLNWMENFADMAHATILHGLVVRDLPDELKPYSDSSIKNW
                     TPLPVEHVETDYGMKTVSVLDTGDPEIKFVNTWALAMPIHWRFSGLRSGFPPDFTDER
                     QEGGGMIRIIDDTHFELFRYSLIRKGNFTGGWLFGLPSNLKGTAGEKPYDKRKYKGWE
                     GLPALEDLVLQESQGEIPDRKLEHLASSDRGVIMLRRLWHQAMQDVAAGKDPKGVIRQ
                     ENGMLEVDTFHGHVKRSELKIGPENMPSSTEGRGLIRDKNGKLVFA"
     gene            3936..>4282
                     /locus_tag="VFY96_15510"
     CDS             3936..>4282
                     /locus_tag="VFY96_15510"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607931.1"
                     /translation="MLTGARSDLRRRRRIFNLALALALSSFSVSLPAQAQLGGKPEKS
                     SFNVSYTQASGAFTPLWVAQETGLFKKHGLDAGLKLLNSQVALQALVAGEVDVISSGP
                     DLVNARLQNVPVK"
BASE COUNT          930 a         1226 c         1159 g          967 t
ORIGIN      
        1 aaaaatgggc agcgccggct cgtagacaat aaagctgcgg cttttgatga gatcgagccg
       61 atccatcgtc aggccgctca cgtcgttttt taaatggatg ccggcgtagt cgccgaaatc
      121 gaggcgccag ctttcctgaa caccgccggc gcgatgcaag cttagcggga caagttttcc
      181 gcggtctttg ccatcggtaa tcctaaattg tttctgccaa tcgccgagag gaaaaagttc
      241 ccgaacggcg atgtcatagg acggcgccaa tgcccatgtt gccggcccaa aaaaagtcgt
      301 ccacaagacg acaatgcaaa ctagaatcgt gcgcccaaac gccatcggca gtgcctccga
      361 actgaaagca gtcgaggcga atagtacaac actgattcta gacgttcaaa ggcgtcgtca
      421 acttttttgc ggagagcgaa gcagtttaag tgattcgtta actgaagctt cgcgctacca
      481 gccagatcac aacgccgacc acggcaacca ccagaacgac cccggtccac acgcccgcct
      541 taaaaatgtc gccgatcacc tgacacccgg tcagagcgag cggagaactg agtagaagaa
      601 acggtttaac agatttcata ggttctcctt agagcgaaaa tagtttatga gagcctcacc
      661 ttgccgtcac tgcggcactt cggattttat ttctagctcg ccaatttctc tcaagctttt
      721 gctcgggctc gcgccgctga tcacgagcac caacctttta gccagattct ccggcaccga
      781 gaccgatgcc gcataagttt gtccattatg gataaccgtt agcgcgagaa catcggagct
      841 gttcaacggc atcgaagcgc tcacatccgt agcatcgagc gcatcttccg gtgcaagtgt
      901 ctgcgtgtcc gctttccact cttggggcgg ccagaagtcg agcgctttga gcttcatgct
      961 ggaacttcct gcacgatctg tatcgatcgg cccatcgcta gttgcatggc agcaaacgga
     1021 aggccaaacg cgctgctaac ctctggtgcg gtaatcaacg aaggatgtcg cttacgcgcg
     1081 gaaaagttgt cgtgccctgt ggatctttac cgacagctgt cagacatttc agcatgcgct
     1141 gcgccgttgt tttgatcatg gctgcggcgt tgttttgata aggtagccct gctcgggctc
     1201 cgcagcactg gcgcggtggt tgcacgagca aagtcgactg cttccgcatc ggagggtaca
     1261 agtgaaaccg atttattgtt ggtcggctct attctcaatt tcgttcgtcc ttgccactag
     1321 cggcgcgaag ctaagcgcgt tggctgcgag tgacagtttg cccgtcgaca aaatcagtct
     1381 ccctgcgggc tttcagatca agccctacgc acaagtaccc aacgcccgct ctatgaccct
     1441 cagcccgaac ggcacgctgt ttgtcggtaa ccgcagtggc ggtaaagttt atgcgattca
     1501 gacccgcgat gaaggggcgc aaattaaaac accattggtc attgccgagc ggctcaatat
     1561 gcccaacggc gtcgccgtca aagatggtgc gctttacgtg gcggaaatca atcgcgtgct
     1621 ccgctacgac gacatcgaag cgcgtgtaaa gaacccgcca caacccgttg tcgtcaatga
     1681 tcgctttcct cgggatcgac agcatggttg gaaatttatt gctttcgggc ccgacggaga
     1741 tctctacgtc ccggtcggtg cgccgtgcaa tatttgcgag ccggacgccg atcgctatgc
     1801 tgcgctcatg cgcatgaaac ccaacggcag caatctggag gtattcgctc acggtattcg
     1861 caacacggtc gggtttgatt ggcatccgca gaccaacgag ctttggttta ccgacaatgg
     1921 tcgcgatcgg ctgggagaca acgtgccgcc ggacgaactg aatcatgcct cgaaagcagg
     1981 gctacatttc ggttatccgt attgtcatgg cggtgatatt cccgatcctg agtacggcaa
     2041 aaaaaaaccg tgcagtgagt tcacgccacc tgcacaaacc ctcggacctc acgtcgcagc
     2101 gctgggcatg cgtttctata ccggttcgat gtttccctcg gagtatctga atcaaatttt
     2161 tattgccgaa catggctcat ggaatcgcag cacgccgatc ggttatcgcg tcactgtcgt
     2221 tgagctcaag ggcaataaag cagtgtcgta ccgacctttc gcccagggtt ggctgcaggg
     2281 aaatcgagcc tggggtagac ccgtcgatgt gctcgtcatg cctgatggcg cactactggt
     2341 ctcggacgac gaggcgggag cgatttatcg gattagctac aaaaaataga gctcgacaga
     2401 cgtggctgcg ctgaaagacc ctcaggcaaa aaccaacttg ccgttcttgt ctcgaatcaa
     2461 acctctgcct tcggttgatg acggcatgtt ctccggacca attttaagct cgcttcgttt
     2521 gacgtggccg tgaaacgtgt ccacttccag catgccgttt tcttggcgga taactccttt
     2581 gggatccttc ccagcggcaa cgtcttgcat cgcttgatgc cagaggcgcc gcagcatgat
     2641 cacaccgcga tcggaagaag cgagatgttc cagtttgcgg tcgggaattt cgccctggga
     2701 ttcctgcaag acgagatctt cgagtgcagg aagcccttcc caacctttgt atttgcgctt
     2761 gtcgtacggc ttttcccccg cggtgccttt caagttcgag ggtaggccga aaagccagcc
     2821 gccggtgaaa tttcctttgc ggatcagact atatcgaaac agctcgaagt gcgtgtcatc
     2881 aatgatgcgg atcatgccgc cgccctcctg ccgctcgtcg gtaaaatccg gcggaaagcc
     2941 ggagcgcagg cccgagaatc gccagtggat cggcatcgcc agcgcccagg tgttgacgaa
     3001 tttgatttcc ggatcgccgg tatcgagcac ggaaaccgtt ttcatgccgt aatcggtctc
     3061 cacgtgctcg accggcagcg gcgtccaatt tttgatgctg ctatcgctgt acggtttcag
     3121 ctcgtccggc aaatcgcgca ccaccaggcc atgcaatatg gtggcatggg ccatgtcggc
     3181 gaagttttcc atccagttta ggtagttgta gttccgcacg tccccatcga cgagcttcag
     3241 ctcgccgtcg gtgcgcgcga gcacgtcgat tttcggcagc ggcggtggat tctctttttc
     3301 cgctcccata taagtccaaa tgacgccgcc ccactcttgg attgggtacc aaacatggcg
     3361 aatgttgttt ttgagcgcgc tgtcgcgcgg ctccagaggt tgatcgatgc aacgcccgcg
     3421 cgtatcgaat ctccatccgt ggtaggaaca catgagcccg tcctcccgca cctgcccata
     3481 ttcgagggat gcgagccggt gcgggcaacg aatgcctagc agacccggct tgcctctgcc
     3541 gtcgcgaaac aagacgagat cttcggagag cattctgacc ggtacaggga catccttcag
     3601 attggccgac aggcacacgg gatgccaata gcgccgcaac atttcgccgg cgcgggttcc
     3661 cggcccggtc cggggcaact cctcaagcaa tttttgatag ccgctctgct ccgcagtttc
     3721 ttctttgatt tgcgccatcc ttgaacctcc acgacgcatg attccagcgc ctgcgtcaaa
     3781 aatctacaga gacaaaggat aggctgtcaa ccttgaaatc ctcggcgtta ggagcgccgc
     3841 atccggcgcc ctagtcaaaa ccaacctcga caacctagat aagtttgacg atggctttcc
     3901 aattctgata tcgagacgca ccagggaggt gaagcatgct tactggtgcg cgcagcgatc
     3961 ttcgccgacg ccgccggatt ttcaacttgg cactggcgct agcactgagt tcattttccg
     4021 tctctttgcc tgcccaggcg cagctcggcg ggaaaccgga aaaatcttcc ttcaacgtct
     4081 cctacaccca agcgagcggc gccttcacgc cactttgggt ggcgcaggaa accggtctgt
     4141 tcaaaaagca tggcctcgat gccggcttga agctgctcaa ttcacaggtg gcgctgcagg
     4201 cgctggtggc cggtgaagtc gacgtgattt cttcgggacc ggatctcgtc aatgcgcggc
     4261 tacagaatgt cccggtgaag ta
//
LOCUS       DASULZ010000753         3292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_898839, whole genome shotgun sequence.
ACCESSION   DASULZ010000753 DASULZ010000000
VERSION     DASULZ010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898839"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VFY96_15515"
     CDS             complement(<1..535)
                     /locus_tag="VFY96_15515"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607932.1"
                     /translation="MKLLRNILLALLILNLVVWTTRAEAKSINFASNTPTLSGTLPLI
                     VAQDFGFFGAENLEVKTVLIRGGPTAMAALVGGGVDYTLVAGVAAVRAIAQNAPMQII
                     SGLQPYMDYTLLGAKNIRTVNDLKGKVVGVTGPGGIAEFAAIEGLAKKGLVRDRDYQI
                     LYGVGNSPSRAQALESGR"
     gene            complement(758..1771)
                     /locus_tag="VFY96_15520"
     CDS             complement(758..1771)
                     /locus_tag="VFY96_15520"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX5607933.1"
                     /translation="MPKLRLSFISAFNERVDPLMKGVVQAEGIELVPTYSHPSETFWR
                     QLKFQEFEVGEMSMSSYLIARERGLDMIALPVFPSRRLFHTELSYHVDSGIQKPEDLL
                     GKRIGVGEYQQTAALWQRGILEHDFGVSQYKVHWFMERTEELSHGGQTGFTPPQGISF
                     QRIPTDKSMASMLVNHELDAAAINSPWKNMPNFIGRSHRISGSDGDWNKVKPLFNDRM
                     AEGKRFFQKWGFLPVNHAYTIRRDVYEKYPWVAFNLYSGFVKAKGHFNAKLADSIPSG
                     LFFGKEYLTMTQEIFGSDPYTYGIKGNRKMLETLIDFSHEQGLIKTKPKIEDLFAGST
                     LDL"
     gene            complement(1969..2982)
                     /locus_tag="VFY96_15525"
     CDS             complement(1969..2982)
                     /locus_tag="VFY96_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607934.1"
                     /translation="MSDLTLSFISNYNERVEPLMNGSVKPEGIEIIPTYSHPSETFWR
                     QLKFQEFHIAEMSMSSYLIARSRGADMIALPVFPSRRFFHAEQSYHLDSGINEPGDLN
                     GKRIGVGEYQQTAALWTRGVLEHDFGVSQYKVHWYMERTEELSHGGVTGFTPPAGISF
                     HRIAPEKSLATMLVNREIDVAPIASHLSRTVNIIDRSTHIRGSEGDWSKVKPLFPDQI
                     AEAKRFVAKHRFVPVNHTYIIRGDLYRQQPGIASKFYQAFLSAKEQAAATLLERTPAA
                     LIFAPEYFAITRSVLGDDPFPYGIKANRSMIDTIASYSHEQGLTPRKMTVEELFAKET
                     LDS"
     gene            complement(3030..>3292)
                     /locus_tag="VFY96_15530"
     CDS             complement(3030..>3292)
                     /locus_tag="VFY96_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607935.1"
                     /translation="FNLYSGFVKAKALAREKLAEFIPSALFFGPEYLAMTRRLIGDDP
                     FPYGIKANQAMLDTITRYSHEQGLTPRQMKPEELFAEQTLSL"
BASE COUNT          739 a          869 c          905 g          779 t
ORIGIN      
        1 ttctaccgga ttccaaggct tgggcacgcg acggactgtt gccgacgcca taaagaatct
       61 ggtaatcgcg gtcgcgcact aaacccttct tcgctaaccc ttcgatcgcg gcgaactcgg
      121 cgattccacc gggcccggta acaccgacaa ctttgccctt gagatcgttg accgtgcgga
      181 tgtttttcgc tcctaggagc gtgtaatcca tgtacggttg gaggccgcta atgatttgca
      241 tcggcgcgtt ttgtgcaatt gcgcgcaccg ccgcgacacc ggcaaccaag gtataatcga
      301 caccgccccc gaccagtgcg gccatggccg tcggcccgcc ccggatgagc accgtcttga
      361 cctccaaatt ctccgcgccg aaaaaaccga agtcttgagc gacgatcaat ggcagcgtac
      421 cgctcaacgt cggcgtgttg gaggcaaaat tgatcgactt ggcctccgcg cgcgtcgtcc
      481 atacaacgag gttcaatatg agtaaagcca gcagtatgtt tcggagtaat ttcatagttc
      541 tgctaaataa gacatttagc ctgtctacgc gagcgccttc ggcacaagga ctctacttct
      601 gcggtgaaga gtgtgccgca actgtcattc tgacgggcca tgccgaagaa ttttgctttg
      661 caaatggcta agaccgagat gcttcgctgc cgctcaacat gacaccgcga ttgttccagg
      721 tagcaaaggt aacgacgact ttgacacgac tgctatgtca cagatccagt gtgctgcccg
      781 cgaagagatc ctcgatcttc ggcttggtct tgatcagtcc ctgctcgtgg gaaaaatcga
      841 tcagcgtctc gagcatcttg cgattcccct tgatgccgta ggtgtaagga tcgctcccga
      901 agatctcctg ggtcattgtc aggtactcct tgccgaagaa caggcctgac gggatgctgt
      961 ccgccagttt ggcgttaaaa tgacctttgg cttttacgaa cccgctataa aggttgaacg
     1021 cgacccaggg atatttctcg tagacatcgc ggcggatcgt gtaggcgtgg tttaccggca
     1081 gaaatcccca cttctgaaag aatctttttc cttccgccat acgatcgttg aaaagtggtt
     1141 tcactttgtt ccaatcgccg tctgagccgg aaattcggtg cgaccgaccg atgaaattcg
     1201 gcatattctt ccacggactg ttgatcgcgg cggcgtccag ttcgtggttc accagcatcg
     1261 aagccatgct tttgtcagtt ggaatgcgct ggaaggagat accttgcggc ggggtgaaac
     1321 cagtctgtcc gccatggctc aactcttctg tccgttccat aaaccagtgc actttgtact
     1381 gcgacacgcc gaaatcgtgt tcgagaatac cgcgctgcca gagcgcggcg gtttgctggt
     1441 actcgccgac cccgatgcgt ttaccgagca agtcctccgg cttctggatc ccggagtcga
     1501 catgatacga gagttcagtg tgaaagagtc gacggctcgg aaacaccggc agcgcgatca
     1561 tgtcaaggcc gcgctcacgg gcaatcaaat aggacgacat cgacatctcg ccgacttcga
     1621 actcttgaaa cttgagctgg cgccaaaaag tctcggacgg atgggaatac gtcgggacga
     1681 gctcgatacc ctcggcctgg acgacgcctt tcatgagcgg atcgacgcgc tcgttgaatg
     1741 cggagatgaa gctcagtctt agtttcggca tataaagttt ttttcgttca aaaccgtaaa
     1801 gtttgcaaag tacccaacgg gttcatttta agaagacctc tcacagagtt taccctgagt
     1861 ctcatcgaag ggttcgagat gacaggagtc ctttggtagc cattccgaac ccttcgcacg
     1921 ctcatgataa agtccacgag gaatcgttct ctacctaact gcgtttgttt acgaatccag
     1981 tgtttccttt gcgaacaact cctcgacggt cattttgcgc ggtgtcaggc cttgctcgtg
     2041 cgagtagctg gcgattgtat cgatcatgga tcggttagct ttgataccgt aaggaaacgg
     2101 atcatccccc aaaacggaac gagttatggc aaaatattcg ggcgcaaaaa tcagcgctgc
     2161 cggggttcgc tccaaaagag tcgccgccgc ttgctctttg gcggagagaa acgcctggta
     2221 aaatttgcta gcgattcctg gttgctggcg gtagagatcg ccgcgaatga tataggtatg
     2281 gtttaccgga acaaaccgat gcttggcgac gaagcgtttc gcttcggcga tttggtccgg
     2341 gaaaagcggc ttgaccttgg accagtcacc ttcactaccg cgaatgtgag tcgagcgatc
     2401 aataatattg accgtgcgcg agagatggct cgcgatcggc gcgacatcga tttcacgatt
     2461 gacgagcatg gtcgccaggc ttttctccgg cgctatgcgg tgaaaagaaa tcccggctgg
     2521 cggggtaaag ccggtgacgc caccgtggct caattcttcc gttcgttcca tgtaccagtg
     2581 aactttgtat tgcgacacac cgaaatcatg ttcgagcacg ccgcgggtcc aaagtgccgc
     2641 cgtctgttga tactcgccga cgccgatgcg cttgccgttt aaatcgccgg gctcattgat
     2701 gcccgaatcg agatgatagg actgctcggc gtgaaaaaac cggcggctgg gaaacaccgg
     2761 cagggctatc atatcggcgc cgcgcgaacg cgcgatcaga taggaagaca tggacatttc
     2821 ggcaatgtgg aattcctgaa atttgagctg gcgccaaaat gtttccgaag gatgagagta
     2881 ggtcggaatg atctcgatgc cttcgggctt taccgagcca ttcatgagcg gctcgacccg
     2941 ttcgttgtag ttggagataa aactcagggt taagtctgac attgtttttg ccgcatcaga
     3001 gggcgcagca tgctgcgctc tacgatttgt cataagctga gggtttgctc ggcaaacagt
     3061 tcctcgggtt tcatctggcg tggcgtcaat ccttgttcat gagaatagcg cgtgatcgta
     3121 tcgagcatcg cctggttagc cttgatgccg tacggaaaag gatcgtcgcc gatgagtcga
     3181 cgcgtcatgg caaggtattc ggggccgaag aacaaagcgc taggaatgaa ctccgccagt
     3241 ttttctcgcg caagcgcttt cgccttgacg aagccgctat aaagattgaa gg
//
LOCUS       DASULZ010000754         2181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1202999, whole genome shotgun sequence.
ACCESSION   DASULZ010000754 DASULZ010000000
VERSION     DASULZ010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202999"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VFY96_15535"
     CDS             <1..264
                     /locus_tag="VFY96_15535"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5607936.1"
                     /translation="RFLDATEIQLTVLYPNEGLNIGLQRDRDWAVALARAYNDWLHHE
                     FLRASSRFQGVALLAPQDPVAAASELERAVKDLGMIAECYLES"
     gene            261..845
                     /locus_tag="VFY96_15540"
     CDS             261..845
                     /locus_tag="VFY96_15540"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5607937.1"
                     /translation="MSPMRGLGLPDFDPIYRTAESLDIPLAVHSGPAVELGLDHMQSF
                     AGVYPLSHPFAQMQQLTSMMIYGVFERFPALRVGYLESGCGWVPYLADRLDESWEHRR
                     KRWPLPIKRSPSEIMRGGNLFYACERDETTLPAVAELIGGDQLLWASDFPHERPWDEF
                     AGDIDALLDRHDLPKSLARKILFDNPRRFYKLDI"
     gene            927..1835
                     /locus_tag="VFY96_15545"
     CDS             927..1835
                     /locus_tag="VFY96_15545"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5607938.1"
                     /translation="MRVLITGAGLIGTHTAKELIERGDEVTFFDFAPKTDYIRRVTGR
                     DVAVLRGDIRDLAGLIDAFQQTRAECVIHLAASVGEANISNVYTGFQVNLVATINVAE
                     AARLIGVRRLVHASTQALYVGEDPIEILSEDSPLDSRERIYNAAKLGCEHVLRTYAAK
                     HKIELALLRFAGVYGYYAVAGGPGVAVQQAVWDALVGKAVTLNVYKSVDFIYVKDLAN
                     GIVLAVHTDPLPHQIYNLGSGTLTTVEDIENALRKIFPEVKTARGKLTPARPGMDITR
                     ARTELGFNPEYKLEAGLRDYVEQLKT"
     gene            complement(1866..>2181)
                     /locus_tag="VFY96_15550"
     CDS             complement(1866..>2181)
                     /locus_tag="VFY96_15550"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX5607939.1"
                     /translation="AGAFSTDDYAALEDTKKPLVSILAETESVPRHLVSVRKDLPEAV
                     VNRLKSVLLAMNQDKEGQQILQQTDKTTKFDSLPGGEEVVRRKLVELYRARSRNPAAR
                     AR"
BASE COUNT          467 a          572 c          644 g          498 t
ORIGIN      
        1 cgtttcctcg atgcgactga gattcaactg acggtgcttt atccgaacga ggggttgaat
       61 atcggcctgc aacgcgatcg cgattgggct gtagcgctgg cccgagctta caatgactgg
      121 ctgcatcatg agttcttgag agcgagttcg cgctttcaag gcgtggcgct gctcgcgccg
      181 caagatccag ttgcggcggc gagcgaattg gagcgagcgg ttaaggatct cgggatgatc
      241 gcggaatgtt acctggagtc atgagcccga tgcgcgggct cggactgccg gacttcgatc
      301 caatttaccg gaccgcggag agtctcgata ttccgctcgc cgtgcacagc gggccggcgg
      361 tcgagctggg cttggaccac atgcaatcat ttgccggcgt ctatccgctc agccatccgt
      421 tcgcgcaaat gcagcaactc acaagcatga tgatctatgg ggtgttcgag cgcttcccgg
      481 ctctgcgggt cggatatctg gaatctggct gtggttgggt gccttatctg gcggatcgtc
      541 tcgacgaaag ctgggaacat cgccgtaagc ggtggccgct gccgatcaaa cgatcgccta
      601 gtgaaatcat gcgcggcgga aatttgtttt acgcctgcga gcgcgacgaa acaacgcttc
      661 cagccgtggc cgagttgatc ggcggagatc agttgctctg ggcatcggac ttcccgcacg
      721 agcggccctg ggacgaattt gccggcgata tcgatgcatt gctcgatcga catgatctgc
      781 cgaaatcgtt ggcgcggaaa attttgttcg acaatccccg gcggttctat aaattagaca
      841 tttgagatgc tcaactttga gatttcaagt tcacatcgga aggggacgcc gcggttgcac
      901 gttcaacaga aaatgagttg tttcccatgc gcgttttaat aacgggcgcc ggcttaatcg
      961 gcacgcacac tgctaaggaa ttgatcgagc gaggcgacga agtcacgttc tttgatttcg
     1021 cgcccaaaac ggattacatc cgtcgcgtga ccggtcgtga cgtcgcggtg cttcgcggtg
     1081 atattcgtga tctcgcaggg ttgatcgatg cgtttcagca aacgcgggcc gaatgcgtga
     1141 ttcacctggc ggcttcggtc ggtgaagcca acatcagcaa cgtctatacg ggatttcagg
     1201 ttaatctggt ggcgaccatc aatgtcgctg aagcggcgcg cctcatcggc gttcggcggc
     1261 tggtccatgc aagcacgcag gcactgtatg tgggtgaaga tccgatcgag attctttctg
     1321 aggactcgcc cctcgacagc cgagagcgta tctacaacgc tgcgaaatta ggctgtgaac
     1381 acgtgctgcg aacctacgcg gcaaaacaca agatcgagtt ggcactgctg cgtttcgccg
     1441 gggtttacgg gtactacgcg gtagcaggag gtccgggcgt agcagtccag caggcggttt
     1501 gggacgcgct cgtcgggaaa gctgtgaccc tcaacgtcta taagagcgtc gattttattt
     1561 atgtgaagga tctcgccaac ggcattgtgc tcgccgtgca tacagatccc ttgccgcatc
     1621 agatttacaa cctgggcagc ggcacgttga caaccgttga agacatcgaa aatgccctta
     1681 ggaaaatttt cccagaagta aaaaccgcgc gcggcaaact gactcccgcg cggccgggga
     1741 tggacatcac ccgtgcgcgc acagagttag gatttaatcc ggagtacaag ttggaggcgg
     1801 ggttgaggga ctatgtcgag cagttaaaaa cataaacgaa gaaaatacgc cccagcgtgg
     1861 caccgtcagc gcgcccttgc tgcaggattt cgggaacgcg cccgatacaa ttccacaagt
     1921 tttcttcgaa ccacttcctc gccgccagga agagaatcga attttgttgt cttgtcggtt
     1981 tgctgcaaaa tctgctgccc ttccttatct tgattcatgg cgagaagaac gcttttgaga
     2041 cggtttacta ccgcctccgg taagtctttg cgcacggaca caaggtgtcg tgggaccgac
     2101 tctgtttccg ctagaatcga taccaatggc ttctttgtgt cttctagagc ggcgtaatcg
     2161 tcggtactga aagctccggc c
//
LOCUS       DASULZ010000755         3147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_492409, whole genome shotgun sequence.
ACCESSION   DASULZ010000755 DASULZ010000000
VERSION     DASULZ010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492409"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VFY96_15555"
     CDS             <1..840
                     /locus_tag="VFY96_15555"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5607940.1"
                     /translation="SQGFMVLEMLNIAEAWPFNDNSMSRADMLHYQVGAKKLAFEDRI
                     RYLEDPRFGDSKIGTLISKEYAAKRRELVDDVMERASARVANQSSDTTYLCAADRDGN
                     AISFIQSVFAPFGSRVIAGDSGVVMNNRLCSFGLDPSKTNSLKPGKRPAHTLNTYMIF
                     RANEVFAIGGSPGADEQPQTNFQIIHDLVDLHMDPQSAVEAPRWSHQPGTPPRDQLPE
                     ALRVEEGFDPATLDGLRKKGHQVSVVDRWSFGSAKVIVRDDENGCWLGGADPRRLAYA
                     LGW"
     gene            1052..2104
                     /locus_tag="VFY96_15560"
     CDS             1052..2104
                     /locus_tag="VFY96_15560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5607941.1"
                     /translation="MKFSLFTEIQCPPGSAPAARLEEFMEQAELADRLGFHTYWIAEI
                     HCQPKFSLLSAPYVVLGAAAQRTKRLRLGVAVNTLPVHHPVQLAEEAAMLDLVSQGRM
                     DFAAGGGHPHSRAYECFGADHKSTHEVMAESLDIIRKAWSEPTLAYNGKFFNITEVIV
                     NPKPVQNPLPSFYMATSSLDGVEVGARLGINMFLPIHTRTPEQVGEYAQAYWNGLKQY
                     GHDAKSKELGLLVPVHVAETTAVAMSRTECGIMSYFKTITDMRADYTDWLTRRGVELP
                     TRLRTAAGAQVAFETVCEKHAVIGDADLVADKIKKLTGQTGATHLLAWFNIGTVPHAL
                     VKDSMERFTKEVTPKL"
     gene            2172..2525
                     /locus_tag="VFY96_15565"
     CDS             2172..2525
                     /locus_tag="VFY96_15565"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006634040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX5607942.1"
                     /translation="MEELIEQKCEACRVGAPSVTPDEIKELQPQVADWKIIHEDGIPK
                     LDRQYKFKNFKDAVAFTDEVGAAAEEEGHHPRITTEWGKVAVTWWTHKIKNLHRNDFI
                     MAAKTDRIYKRYAQL"
     gene            complement(2587..>3147)
                     /locus_tag="VFY96_15570"
     CDS             complement(2587..>3147)
                     /locus_tag="VFY96_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607943.1"
                     /translation="MALWGKGMVLYQDKKDYAGAREIFERLLNLIPPGEERNEVVKVL
                     GEIPASGGIPNRSAATNKNSGATSPSSAQITGKITIDPKLKGSIDPNAALFIIARPAS
                     GAGGPPLAVKKIDRPSFPVDFTLSQENVMMPGISFAGKINITARLDKDGNPVTRTPGD
                     ISGDYKKNPIEVGTKNVDVMIDQVMK"
BASE COUNT          738 a          829 c          886 g          694 t
ORIGIN      
        1 tctcagggtt ttatggtgct cgagatgctc aacatcgccg aggcctggcc attcaatgac
       61 aactcgatga gccgagccga tatgcttcat tatcaggtgg gagcaaaaaa actcgcgttc
      121 gaagatcgca ttcgttatct cgaagatccg cggttcggcg attcgaaaat cggcacattg
      181 atttccaagg aatatgcagc caaacgccgc gaactcgtcg atgatgtcat ggagcgagct
      241 tcggccaggg tcgcgaacca aagcagcgat accacctatc tctgcgctgc ggatcgcgac
      301 ggtaatgcga tttcttttat acaaagcgtt ttcgctccct ttggctcgcg ggtaatcgcc
      361 ggcgattccg gggtggtcat gaataaccgc ctgtgcagct ttggtttgga tccgagcaag
      421 accaattcgc tcaagccggg caagcgtccg gcgcacacct tgaataccta catgattttc
      481 cgcgccaacg aagtttttgc gatcggcggc agtccgggcg ccgatgagca gccgcagacc
      541 aattttcaaa tcattcacga cctcgtcgat ttgcatatgg atccgcagag cgccgtcgaa
      601 gcgccgcgct ggagccacca acccggcact ccgccacgcg atcagttgcc cgaggctctg
      661 cgcgtggaag agggattcga tccggcgacc ctggacggct tgcgcaagaa agggcatcag
      721 gtctcggtgg tcgatcgctg gtccttcggc agcgcgaaag tgatcgtgcg cgatgatgag
      781 aacggctgct ggctcggcgg cgccgatccg cgccgactcg cttacgcctt ggggtggtag
      841 ctccatttca aggtcttcgt tcaaggaata gagaaaacta acgagcgtag ccgatatatt
      901 gcgagtggct tcgtgtgtta gagtttctca gatgacgatc ggcgtcaccg cagcaaaatt
      961 gtcctctgca gtaattaaaa atccaattcg tcgagggctg tacacgtaat taagtgcaaa
     1021 tcgcattctt cacagaagag gaacagctgc cgtgaaattc agcctgttta cggaaattca
     1081 gtgtccgccc ggctctgcac ccgcagcaag gctcgaagag ttcatggaac aagcagagct
     1141 cgccgaccgc ttgggatttc atacctattg gatcgccgag attcattgcc agccgaaatt
     1201 ttcgctgctt tcggcgcctt acgttgtgct cggtgccgcg gcgcaaagaa ccaagcgtct
     1261 tcggcttggt gtcgcggtga acacgctgcc agtgcatcat ccagtgcagc tcgccgaaga
     1321 agcggcaatg ctcgatctgg taagccaggg tcgaatggat tttgccgcag gcggcggtca
     1381 tccgcacagc cgcgcctacg aatgctttgg cgccgatcac aagtcgactc atgaggtcat
     1441 ggccgaatca ctggacatca ttcgcaaggc atggtctgaa ccgacgcttg cctacaacgg
     1501 caaatttttc aatattacgg aagtgatcgt caatcccaag ccggtgcaaa atccgttgcc
     1561 gtccttttat atggcgacca gctcgctcga cggcgtcgaa gtcggcgcgc gactcggaat
     1621 caatatgttt ttgccgatcc atactcgcac tccggaacag gtcggcgagt atgcacaggc
     1681 atactggaat ggactaaaac agtatggcca tgatgccaaa agtaaagaac tcgggttgct
     1741 cgtgccagtg cacgtggcgg agaccacggc ggtggcgatg tctcggacgg aatgtggaat
     1801 catgagctac tttaaaacga ttacggacat gcgcgcggat tacactgact ggctgacgcg
     1861 gcgcggcgtg gaattgccga cccgcttgcg cacggctgcg ggagcgcagg tggcgtttga
     1921 gacagtttgc gagaagcatg cagtgatcgg cgatgcggat ctcgtcgccg acaaaatcaa
     1981 aaagttgacc ggccaaaccg gcgctaccca tcttctggcc tggttcaata tcggcacggt
     2041 accgcacgcg cttgtaaagg attcgatgga gcgatttact aaagaagtaa cgccgaagct
     2101 gtgatcgaac gacaccagat ggaaaaatgt agcggcgcgg cgggcgcctg aggttattgg
     2161 agggattacc catggaagaa ttaatcgaac aaaaatgtga agcttgccgc gtcggcgcac
     2221 cgtcagtgac accggacgag atcaaagaac ttcaacccca agtcgcggac tggaaaatta
     2281 tccatgaaga cggcattccg aaattagacc ggcagtacaa attcaaaaat tttaaagacg
     2341 cagttgcttt taccgacgag gtcggcgcgg cagcggaaga agaaggccat cacccgcgga
     2401 ttaccaccga atggggcaaa gtcgctgtta cttggtggac gcacaaaatc aaaaacctgc
     2461 acagaaacga cttcatcatg gccgcgaaaa cggatcgaat ctataaaaga tacgcccaac
     2521 tataatttct gttgtacgat ctatattaag attggagcag ggaagaggct ctctcgagtc
     2581 ctcaactcat ttcatcacct gatcgatcat cacatccacg tttttcgtgc ccacttcgat
     2641 aggattcttt ttgtaatctc cgctgatatc gccaggggtg cgggtgacgg ggttgccgtc
     2701 tttgtcgagg cgagcggtga tattgatttt gccagcgaag gatatgccag gcatcatgac
     2761 attctcttga ctgagggtga aatcgacggg gaagctcggg cgatcgattt tttttacggc
     2821 aagcggcggg ccaccggcgc cactcgcggg gcgggcgatg ataaacaatg ctgcgttcgg
     2881 atcgatgctt cctttgagtt tgggatcgat ggtgattttg ccggtgatct gcgcggacga
     2941 tggcgacgta gcgcccgaat ttttgttcgt agcggccgag cggttcggga taccgccact
     3001 cgccggaatt tccccgagca ctttcaccac ctcgttccgc tcttcacccg gcggaatgag
     3061 atttaaaagc cgctcgaaga tttcgcgcgc gccggcatag tctttcttgt cctggtacaa
     3121 gaccatacct ttgccccaga gtgccat
//
LOCUS       DASULZ010000756         2095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_87241, whole genome shotgun sequence.
ACCESSION   DASULZ010000756 DASULZ010000000
VERSION     DASULZ010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87241"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VFY96_15575"
     CDS             <1..606
                     /locus_tag="VFY96_15575"
                     /inference="COORDINATES: protein motif:HMM:NF020456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetone carboxylase subunit gamma"
                     /protein_id="HEX5607944.1"
                     /translation="VPERIPLPEGSLLVDYVAGGGGFGDPIERDTNAVLQDFCRGWVS
                     REVAERIYGVILSTAGKAVDEAATKRRREEIRNERKQKSAPASARSDDVSRNSWRSLL
                     KFHAALQIATDGKHKVIRCARCGHVFCSAEENYKRFALHRIVHLSEIMPPLASGDPYI
                     GEYHLYSCPDCATQLQVDMFSPPLGGDPVLWDTRIDADGLN"
     gene            626..1594
                     /locus_tag="VFY96_15580"
     CDS             626..1594
                     /locus_tag="VFY96_15580"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607945.1"
                     /translation="MRLPRTVSFLLVVMLVAWFRPGFAQERITVGLTTRTGSTSLPFV
                     IAEEKGFFKSEGVNAIVVIMQNQVVVNGVVTRNVDYGGTFSNFVGAALSNLPVRIVMS
                     AMDGSDHYLVTSANIKRVEDLKGKKFGISSFGGTPHSEAIMILRKFGMSPEKDVTFLQ
                     IGGSSSRYAALESGSIDAAMLVPPFNNFAVKHGFHQLVSFNDIMKIPLGGLAVHTQRM
                     KEKPDEIVKMIKAVLKATDYIRTRKADILNLIETQWGIKEPDIRESIYRDMVGLFSRD
                     GVASEETMKNVVQLVRDTRKSKDDKTLADIFDWSFAKKAQAELKLK"
     gene            1722..1844
                     /locus_tag="VFY96_15585"
     CDS             1722..1844
                     /locus_tag="VFY96_15585"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX5607946.1"
                     /translation="MWGRLQRKQILGVQFYRQKPIGNYIVDFYAPVVNLIVEID"
BASE COUNT          510 a          566 c          567 g          452 t
ORIGIN      
        1 gtccctgagc ggattccgct gccggaagga agtttgctgg tggattacgt tgcgggcggc
       61 ggcggttttg gcgacccgat agagcgggac actaatgccg tgctgcagga tttttgccgc
      121 ggctgggtga gtcgcgaagt cgccgaaagg atttatggcg tgatcctaag cacagcgggt
      181 aaagccgtgg acgaggcggc gacgaaaaga cggcgtgaag aaatccgcaa tgagcgtaaa
      241 caaaaaagcg cgccagcgag cgctcgcagc gacgatgttt cacgaaatag ttggcgttca
      301 ctgctgaaat ttcatgcggc gctgcagatc gccactgacg gcaaacacaa agtcatacgc
      361 tgcgcccgct gcggtcacgt cttttgcagc gccgaagaaa attacaagcg cttcgcgctc
      421 caccggatcg tgcatttgag cgaaatcatg ccgccacttg cgtccggtga cccctacata
      481 ggcgaatatc atctctacag ctgccccgat tgcgccactc agctgcaagt ggacatgttc
      541 agcccacctc ttgggggcga tcccgtgctc tgggacacgc ggatcgatgc tgacgggttg
      601 aactgacgca gcgaggtaca aacccatgag attgccgcgc acggtttctt tcttgctcgt
      661 tgtgatgctc gttgcctggt tccgacccgg tttcgcccag gagcgcatca ccgtcggcct
      721 cacgacccgc accggcagca cgtcgctgcc tttcgtgatt gccgaagaga agggtttttt
      781 caaatccgaa ggtgtgaatg ccattgtcgt catcatgcaa aatcaggttg tggtcaatgg
      841 agttgtgacg cgcaacgttg attacggcgg aaccttttct aatttcgtcg gcgcggcgct
      901 atccaatcta ccggtccgta tcgttatgtc ggccatggat ggatcggatc actatttggt
      961 caccagcgcc aacatcaaac gagtggaaga cctgaaaggt aaaaaatttg gcatcagcag
     1021 cttcggcggc acgccccaca gcgaagcgat catgattctg cgcaaattcg gcatgagtcc
     1081 tgagaaggac gtcacctttc ttcagatcgg cggtagctca agtcgctatg ccgcgctgga
     1141 gagcggctcc attgacgcgg ccatgctcgt gccgccgttt aacaacttcg ctgtcaaaca
     1201 cggctttcac cagctcgtct cgtttaatga catcatgaag attcctctcg gcggccttgc
     1261 cgtccacact cagagaatga aagaaaagcc cgatgagatt gtgaagatga tcaaagccgt
     1321 tttgaaagcg acggactaca tccgcacgcg caaggccgac atcttaaact tgatagaaac
     1381 tcaatggggc atcaaagaac ccgatatccg cgagagcatt taccgtgata tggtcggcct
     1441 attcagccgc gacggcgtcg cttccgaaga gaccatgaag aatgtcgttc agctcgtccg
     1501 cgacacccgc aaatccaaag acgacaagac tctggcggat attttcgact ggagcttcgc
     1561 caaaaaagcc caagcggaac tgaagctcaa ataaatgcga gtgcgcagtt cagtctaagt
     1621 gaatttcaaa cgaccgcagt tttattcatg ctccgataca gtcgtcacct caaaggaaca
     1681 gcccgacgat tgcggtcaaa cttacggagg cagaacagcg aatgtggggc cggctgcaac
     1741 gcaagcagat cctcggtgtg caattctata ggcaaaagcc catcggcaac tatatcgttg
     1801 atttctatgc accggtcgtg aacctgattg tggaaatcga ttgatcacaa catctggaac
     1861 cggagcaggc aaatcgtgac gagcgccgta ccgcctgttc aaccagcttg gtttgagagt
     1921 tttgcgcttt aacgatcgcg aagtactgct ggagctcgac tcggttgcag aaacaatcta
     1981 tcgtgcggtc gaggaaaaaa tccccctaaa tcccctttgc caaaggggga caattcttgt
     2041 ctgaatgcac gcggactcaa cgaagccgcg tccctgtctt acccctcatt gaaaa
//
LOCUS       DASULZ010000757         2502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_999508, whole genome shotgun sequence.
ACCESSION   DASULZ010000757 DASULZ010000000
VERSION     DASULZ010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999508"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1995
                     /locus_tag="VFY96_15590"
     CDS             <1..1995
                     /locus_tag="VFY96_15590"
                     /inference="COORDINATES: protein motif:HMM:NF011071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming)/trehalose-phosphatase"
                     /protein_id="HEX5607947.1"
                     /translation="SAEQMDQFYLGFCNATIWPLFNYFTSYTVYQPGFWEQYKQINSL
                     FADALKAVLRPDDIVWVHDYHLKLLPRLLKARHSQLSVGFFLHIPFPSFEVFRLLPAE
                     WRRGILEGILGADLIGFHTYEYTHHFLQSTLRILGYEHHMGQIETPDHVTRVDTFPMG
                     IDVQKFAAASLNEDIAREGRELKEMLSGVRLILSVDRLDYSKGILNRLEGFELFLEVN
                     PEYHGKVALLMVVVPSRIGVVQYELMKRQIEELVGKINGRFGRVGWTPVVYQYRQVPF
                     TSLAALYLVGDVCLVTPLRDGMNLVAKEYVATRGDGAGVLILSEMAGAAKELPEAIIV
                     NPNDRREIAAALKEALETPADEQQARNRLMQRRLQRYNVTRWARDFLTSLVAMREIQT
                     PLESKLLSVDATREILDHYQSSERRLLFLDYDGTLTPLVRHPAMAKPDRKIRELLKTL
                     TANPKNTVVIASGRERQILQEWFGSLPLGLVAEHGAWIRFAGQDWQHAKPFSTEWKRD
                     LLPILEIYADRLPGAFVEEKEDAVAWHFRMADPEQAELRASELVDHMTHLTAKTDLQV
                     VLGSKVVEIRRAGVDKGSAALSWLGGGDYDFILGIGDDTTDEDLFRSMPTGALTIRVG
                     ISATLARYTVSSSAEVIELLGALAREAQTDGRLEAGGRPR"
     gene            complement(2001..2438)
                     /locus_tag="VFY96_15595"
     CDS             complement(2001..2438)
                     /locus_tag="VFY96_15595"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEX5607948.1"
                     /translation="MGFHGIAGTGEVLSPLFGFLSGFLNGVTKAAGPTLAIYLYSLKL
                     DKRTFVKSIATIFVTTKLSQLVAISTWNLFNWYTIKLSVLVLLFTLAGFYAGLKTQDR
                     VDQGNFNRALLVLLALVGLVLIVRAVTQGTEIRISLNKLRNTC"
BASE COUNT          590 a          628 c          711 g          573 t
ORIGIN      
        1 agcgccgaac agatggacca gttctattta ggtttttgta acgctacgat ttggcctctg
       61 tttaattact tcacctctta caccgtttac cagccgggat tttgggagca gtacaagcag
      121 atcaacagtc tttttgccga cgctctcaaa gcggtcctga gacctgacga tatcgtctgg
      181 gtacacgatt atcacctcaa gctgttgccg cgtctgctca aagcgcgtca ttcccagctc
      241 tcggtgggtt tttttctcca cattcctttt ccatccttcg aggtattccg gttattgcca
      301 gcagagtggc gccgcggcat tctcgagggg attttgggcg ccgacctgat cggatttcat
      361 acctacgaat acacgcatca ttttttgcaa tccacgctcc gtattctcgg ttacgaacat
      421 cacatgggcc aaatcgagac cccggatcac gttacccgcg tcgatacctt cccgatgggt
      481 attgacgtgc aaaagttcgc tgccgcctca ctcaacgaag atatcgcccg cgaaggacgg
      541 gaactcaaag agatgctcag cggtgtgcga ctgatcctgt ccgtggatcg gctcgattat
      601 tcaaaaggaa ttttgaaccg tctggaaggc ttcgaattgt ttttagaagt aaatcccgag
      661 taccacggca aagtggcgtt gctgatggtt gtcgtaccgt cgcgcatcgg ggtggttcag
      721 tacgagttaa tgaaacgaca gattgaagag ctggtcggaa aaatcaacgg ccgattcggc
      781 cgagtcggtt ggactccggt cgtctatcaa tatcgccagg tgccgttcac ttctttggcg
      841 gcgctctatt tggtcggtga cgtttgcctg gtgacgccgc tgcgggacgg catgaacttg
      901 gttgccaaag aatatgtggc aactaggggt gatggcgccg gggtgctgat tctcagtgag
      961 atggctggtg ctgccaagga actgcccgaa gcgattatcg tcaatccgaa tgaccgcagg
     1021 gaaattgccg ctgcattgaa agaagcgctg gaaactcccg ccgatgagca acaggcgcgc
     1081 aaccgtctga tgcagcggcg tttacaaaga tacaatgtca cacgctgggc gcgcgatttt
     1141 ttaacctcgc tggtcgccat gcgcgagatt cagacgccac ttgaatcgaa acttttatcc
     1201 gtcgacgcca cacgggaaat cttggatcac taccaatcga gcgagcggcg gctgctgttt
     1261 ctcgattatg acggcacctt gacgccgctg gtacgccatc cggctatggc gaagcctgac
     1321 cgaaagatcc gcgagctgct taaaacgctc acagcgaatc cgaaaaatac tgtcgtgatt
     1381 gccagcggac gggagcgaca gattctccaa gagtggttcg gctctttacc gctcggcttg
     1441 gtggcagaac atggtgcatg gatcagattt gccgggcaag attggcagca tgccaaaccg
     1501 tttagcaccg aatggaaacg cgacttgctt ccaatcctgg agatatacgc cgatcgattg
     1561 ccgggagctt tcgtcgaaga aaaagaagat gcggtcgcgt ggcattttcg tatggcggat
     1621 ccggaacaag ctgaactgcg cgcctccgag ctggtcgatc acatgaccca cctcaccgct
     1681 aagactgatc tgcaggttgt acttgggagc aaagtcgtag aaatcaggcg ggcgggagtc
     1741 gataaaggaa gcgcggcgtt gtcctggctg ggcggcggcg attacgattt tattctcggc
     1801 attggtgatg acactaccga tgaagatctt tttaggagca tgccgaccgg ggcgctgacc
     1861 attcgcgtgg gaatctccgc aaccctggcg cggtatacgg tttccagcag cgctgaagtg
     1921 atcgagctgc tgggagctct ggcgagggaa gcacagaccg atgggcggtt ggaagcgggc
     1981 ggccggccgc ggtgacgaaa ttagcaggtg tttctgagct tattgagact aatcctgatt
     2041 tcagtgccct gagtcactgc acgaacaatc agtactaggc cgactagagc caataatacg
     2101 agcaaggcgc gattgaagtt tccttggtcg actcgatctt gggtttttaa tcccgcataa
     2161 aaaccggcaa gggtgaagag cagcacgaga acggaaagct tgatggtata ccagttgaaa
     2221 aggttccaag ttgagatcgc gactaactgg ctcagttttg tggtgacgaa gatggtcgca
     2281 atggacttga cgaaagtgcg tttatcgagc ttgaggctat aaaggtaaat cgccagcgtc
     2341 ggaccggcgg ccttggtgac gccgttgaga aatccgctca aaaagccgaa tagaggagaa
     2401 agaacttctc ctgtgccggc gataccgtga aatcccactt aagcacgctc gcgagcacga
     2461 agagcaaaac catcgcgccg agacaaacat tgagtagcca ta
//
LOCUS       DASULZ010000758         2860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_899255, whole genome shotgun sequence.
ACCESSION   DASULZ010000758 DASULZ010000000
VERSION     DASULZ010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899255"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(450..803)
                     /locus_tag="VFY96_15600"
     CDS             complement(450..803)
                     /locus_tag="VFY96_15600"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607949.1"
                     /translation="MEALCSSTMLLLRVVSLQIQALAYNTKKLKREEAPKSWEDVTNP
                     KYKGIVEKYSCVHMTSFPFEWMIALMHMIGEGVFDRYPKLRVGFMEGACGWLPFWSER
                     LDEHFHKLRPQWPLL"
     gene            complement(883..1083)
                     /locus_tag="VFY96_15605"
     CDS             complement(883..1083)
                     /locus_tag="VFY96_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607950.1"
                     /translation="MGKLAKEFYPKAKQEGALVVYTVWNVDHVRSLTEAFTKWFPGIT
                     ASYWQATRSEVTTRTLTEYQGN"
     gene            1592..2044
                     /locus_tag="VFY96_15610"
     CDS             1592..2044
                     /locus_tag="VFY96_15610"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX5607951.1"
                     /translation="MNEDVFAGQWRELKGTLRSWWGNLSDDDFEWVGGQKDRLIGLLQ
                     QKYGWTREQAQQDVDRRLEEYQGSAMGGLNNLKSKTYAAGETAANKAREAFSSAANGF
                     ETARSYVTENDFSTMAADFRDFVRKYPFQTVLIAAGLVYLLFRGRDRD"
     gene            complement(2219..>2860)
                     /locus_tag="VFY96_15615"
     CDS             complement(2219..>2860)
                     /locus_tag="VFY96_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607952.1"
                     /translation="VSERPTDSKRIKRSPIPAKFFWPAATLLLVLLAFYCGAYLQRRE
                     MALTADAYSKHLQTKDQMIQNQEIQMSRLRQQLNDNTNQLGELKTKLQESQKTVAATQ
                     QRLAAANHQIERTNTTRSAAATRVGSRARASSQTTPLPTTRRTEAGVYETVRETSVYD
                     NPSSHARVISQIRGGTRINVVNSGSEWLEVHSKHGNPPGYVRSNDARPISRSN"
BASE COUNT          641 a          695 c          822 g          702 t
ORIGIN      
        1 tcaagccgca ggatctcatg gatcggcgct acttagccga gatggagagc agcgggtttt
       61 tcaaccaagt ctggaatgaa aagcgttgag cttgaatatc gagaatggaa gatggaggat
      121 ggaagatcga aaaggccgcc aatcatgatt ctccatcctc gattctcgat gttaggcttt
      181 gtattatccg gccggcagat cctgacctct cagattgaac caattgcgcg cgttttcacc
      241 gaggacgtgg cgtagcttgt cttcggtcat gccgggaatt ttcttcacat cttttactga
      301 gtcgggaatt cggagtccca gtgccggtaa tcggaagcgt acatcacctg agtcatgccg
      361 atattgtcga gcacagaggg caagatcttt cttccggctc gcaggtgaag gcgacttgcg
      421 gactcttgat gatctcggac ggttttcgtt taaagcagtg gccattgagg ccgcagctta
      481 tggaaatgtt catctaggcg ttccgaccaa aacggcagcc agccgcaagc gccttccata
      541 aagccgactc tgagcttcgg atagcgatcg aagacaccct cgccgatcat gtgcatgagc
      601 gcgatcatcc actcgaatgg aaacgacgtc atgtgcacac agctgtattt ctccacgatc
      661 cccttgtatt tcggattggt cacgtcctcc cagctcttcg gcgcctcttc gcgcttgagc
      721 ttcttggtgt tgtaagcgag tgcctgaatc tgcaggctca cgacccggag caatagcatc
      781 gttgacgaac agagcgcttc catgaacggt ttgataagcc tgggtcgccc gggcgttgtg
      841 cagaacccac ggggcttcgg taacgattac atccacgctc gattaattcc cttgatactc
      901 tgtcagggtg cgggtcgtca cttcggaccg agtcgcctgc cagtacgacg cggtaatccc
      961 gggaaaccat ttggtgaagg cttcggtcag cgagcgaacg tgatcgacgt tccaaaccgt
     1021 gtaaacgacc agcgcgcctt cctgtttggc cttgggataa aactctttgg ccagtttccc
     1081 caaaccgcgg attggtttcc tgccgctgtg gagcaacggc gaaagagttg ccgatcagga
     1141 cgaccaaaat caaagccggg aagatcgatt gcttcgtcat ggagactccg tttctttgaa
     1201 tcgcgcggca atggagattt tgcagcgcga atgttcggcc gagttcctgt tatgcgcacc
     1261 cggattggac tctccatcgt tcagtcaagt accgaatctc ggacgaaaag aatgaaagct
     1321 cctaacacac ggaggaattt ctcgattcat tcatggtttt tcacacacgc agaaaaaaac
     1381 tgcgattgaa attactgaaa aatcgtcaag cgctttgtgg catcgcggtt gcacaaagaa
     1441 ggggcagtcc atgcgtgtgg gcgaataaaa ataggggggc aaaacgatgg gcagtatctt
     1501 ttacataatc ggcgtagtgg tcgtcgtatt gtttattctt ggatatctgg gtctccgata
     1561 aggagaccga tagcggaaag gggaaatagc aatgaatgag gatgtattcg ctggacaatg
     1621 gcgcgaattg aaggggacgc tcagatcgtg gtggggcaat ttgagtgatg acgattttga
     1681 atgggtaggc ggtcagaagg atcgcttgat cgggttattg cagcagaaat acggttggac
     1741 tcgcgagcaa gcgcagcagg atgttgatcg gcggctggag gagtaccaag gctcggctat
     1801 gggcggtctg aataatctga aatcgaaaac ctatgctgcg ggtgaaactg ccgcgaataa
     1861 agcgcgggaa gcattttcat cggctgcgaa cggattcgag accgctcggt catacgttac
     1921 cgagaacgat ttcagcacca tggcggcaga cttccgggac tttgttcgaa agtatccctt
     1981 ccaaacggtg cttatcgccg cgggtttggt ttacctgtta ttccgaggcc gcgatcgcga
     2041 ttaagtgctg acttcgcacc ctgaaagctt tgtagggagc cgaaaaggct ccctacgttt
     2101 ttgcctaacg cgcaacgact tgctcaaccg ccggcgtcca cacggttacc cgtgtgaacg
     2161 ggcgctgttt aatatttgcg tgataaccgt tggtttgacg aattaatgtc gtaaactgtt
     2221 aattacttcg gctaatagga cgggcgtcgt tcgagcgtac gtagcctgga ggatttccgt
     2281 gctttgagtg tacctcgagc cactcgctgc cagagttgac cacgttgatg cgcgttcctc
     2341 cgcgaatttg cgaaatcact cgggcgtgag atgacgggtt atcgtagacc gaagtctcgc
     2401 gcaccgtctc gtagacaccc gcttcagttc ggcgcgtcgt gggcaacggc gtcgtttggg
     2461 atgaggctcg cgcgcgagac ccaacacgcg tggcggcggc cgatctggtt gtgttggtac
     2521 gctcgatctg atggttcgca gcggcgaggc gctgctgtgt agcggcaacc gtcttctgac
     2581 tctcttggag cttggtcttt aattcgccga gctgatttgt attgtcatta agttgttggc
     2641 ggagccggct catctgaatt tcctgatttt ggatcatctg atctttggtt tgcaggtgtt
     2701 tcgaataagc gtccgccgtg agggccatct ctcttcgttg taggtaagcg ccacagtaaa
     2761 aggccagcaa aaccagaagc agagtcgcgg cgggccaaaa aaatttggca gggatgggcg
     2821 atcttttgat gcgcttcgaa tcagttggcc gctcggacac
//
LOCUS       DASULZ010000759         3124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_695948, whole genome shotgun sequence.
ACCESSION   DASULZ010000759 DASULZ010000000
VERSION     DASULZ010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695948"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VFY96_15620"
     CDS             <1..347
                     /locus_tag="VFY96_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5607953.1"
                     /translation="TRSSILEVMNEDYVQTARAKGLQEKNVVWRHVTSNALIPVITVV
                     GLELGTLLSGSIIVETVFAWPGSGSLLITALNARDYPLVTGIVMTYTLAFVAINLIID
                     GLYAVVDPRIRY"
     gene            316..1209
                     /locus_tag="VFY96_15625"
     CDS             316..1209
                     /locus_tag="VFY96_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010140748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5607954.1"
                     /translation="MQSLTLESATDKSVVWPRLLPRLHVKAFTGGVIVLLFIVIGIIG
                     PSIAPFDPNKQELTAMMKAPAGIGAPHSLGTDNLGRDILSRVIYGSRVSLLVAFAVVF
                     VSGFIGIALGAISGYFGGKVDFVIQKLVEVVWAFPPLLLGITIMAFLGQGLFNLILAL
                     VAQRWIPYCRVVRGQTLSLRNRDFVAAAQCLGASNRQIITRHIIPNLIQTSLVIGTFA
                     MASSIIAEASLSFLGVGVPPEIPTWGTMLADARIYISTAWWLPLFPGLCIFVTVLGIN
                     LLGDALRDILDPRLKRSGAKI"
     gene            1333..2736
                     /gene="pyk"
                     /locus_tag="VFY96_15630"
     CDS             1333..2736
                     /gene="pyk"
                     /locus_tag="VFY96_15630"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX5607955.1"
                     /translation="MRRTKIVCTIGPACSSDKVLEGLMRAGMNVARLNFSHGDYASHR
                     RIIRKIRQLERKLDQPVAILQDLPGPKIRIGAVAGDRVRLQNRRPFVLTTQKILGSEL
                     AVSVNFSGLTAAVNKGDPILLGDGEIELETVEVSDREVKCRIVVGGILGSHKGIHFPK
                     SSLNIRALTRHDKQDMAFGIEQKVDMVALSFVRDAEDILYARRDLKQRGASIPLIAKI
                     EKHEALEHIDAILETVDGIMVARGDLGLEIAQERIPAVQKMIIAKANRLGKPVITATQ
                     MLRSMVWNPRPTRAEVTDIANAILDGTDALMLSEETAAGDYPLEAVKTMAQVAEETET
                     ILEPRQRFPDLEKTVPEAISLGAISLARELKVKAFLIPTTSGSTARLIARYRPRQPII
                     AVSPERQTVKMLCLVWGVHPVSTRGFKSTDEMVRMLQQKALESGLVKRGDLVAITAGL
                     PLHQTGTTNMITAKVIE"
     gene            2901..>3124
                     /locus_tag="VFY96_15635"
     CDS             2901..>3124
                     /locus_tag="VFY96_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607956.1"
                     /translation="MSEALKAFEEELQSKNFKGYWQNQQGDVNREPVPSFEACFWKGK
                     DLYAAMEKAGDVVGLDVSFRRVIQLWNPAL"
BASE COUNT          669 a          887 c          877 g          691 t
ORIGIN      
        1 tcacccgctc ttccatattg gaggtgatga acgaggatta tgtgcaaacg gcgcgcgcta
       61 agggcctaca ggaaaaaaat gtcgtatggc gccatgtcac gagcaacgcc ctgattccgg
      121 tgatcaccgt ggtaggactc gagctgggga cgcttctcag cggctccatt attgtcgaga
      181 cggttttcgc ctggccggga agcggcagtt tgctgatcac cgcgctgaat gcccgtgatt
      241 acccgctggt caccgggatc gtgatgacct acacactcgc cttcgtcgcg atcaatctga
      301 tcattgatgg tctttatgca gtcgttgacc ctcgaatccg ctactgacaa gagcgtcgtc
      361 tggccgcgcc tcctgccgcg gcttcacgtc aaagcattca ccgggggcgt gattgttttg
      421 ctgtttatcg taatcggcat catcggcccg agcatcgcgc cgtttgatcc gaacaagcag
      481 gagctgaccg cgatgatgaa agcaccggcg ggaatcggcg cgccgcattc acttggcacc
      541 gacaacttgg ggcgcgatat tttgagccgc gtcatttacg gctcgcgcgt gtcgctgctc
      601 gtcgccttcg cagtggtttt tgtttccggg ttcatcggga tcgcactggg cgcgatttcc
      661 ggttatttcg gcggtaaagt cgattttgtc attcaaaagc tcgtcgaggt cgtctgggct
      721 tttccaccgc tcttactggg catcacgatc atggcgttct tgggacaggg cttattcaat
      781 ctaattttgg ccctcgtggc ccagcgctgg attccgtatt gccgcgtcgt ccgcggtcaa
      841 acactttcct tgcgcaatcg cgactttgtc gccgcagcgc aatgtttggg cgcaagtaat
      901 cgccagatca tcacgcgcca catcattccc aatttgatcc agacctcgct ggtcatcggc
      961 accttcgcca tggcgtcctc gatcatcgcg gaagcgagtc tgagctttct cggcgttggc
     1021 gtaccgcccg agattcccac ctgggggact atgctcgcgg acgcgcgtat ctacatcagc
     1081 accgcatggt ggctgccgct ttttcccggc ctgtgcattt tcgtgaccgt gctcggcatt
     1141 aatcttctcg gcgacgccct gcgcgatatc ctcgacccaa ggctgaaacg atccggcgcg
     1201 aagatataag atcaactgga gtaatggcgt cgttgagcgt tgctctagac gtattactgc
     1261 aacattccag taatccattc cgccgttttc cttttcgttg attctccgac actctcgtgt
     1321 acattgggtg aaatgcgccg cacaaaaatt gtttgcacca ttggaccggc ctgttcgagc
     1381 gacaaagttc ttgagggttt gatgcgcgcc ggcatgaacg tcgctcggct caatttcagc
     1441 catggcgatt acgcttcgca ccggcgcatc attcgaaaaa ttcgtcaact cgagcgcaag
     1501 ctcgatcagc cggtggccat ccttcaagat ctgccgggac caaaaatccg catcggcgcg
     1561 gtcgccggcg atcgcgtccg ccttcaaaac cgccgaccgt tcgtgttgac cacgcaaaaa
     1621 atcctcggca gcgaacttgc tgtttcagtg aactttagtg gcttgacggc agcggtgaac
     1681 aaaggcgatc cgatcctgct tggggacggc gagatcgagc tggaaaccgt tgaagtgagc
     1741 gaccgcgagg tcaagtgtcg catcgtcgtt ggcgggatac tcggctcgca taaaggcatt
     1801 cactttccga aaagcagcct gaacatacgc gcgctgacgc gccatgataa gcaggatatg
     1861 gcgtttggta tcgagcagaa ggtggacatg gtggcgttgt cattcgtgcg cgatgccgag
     1921 gacattcttt atgcgcggcg agatctgaaa cagcgcggtg cgtcgatccc attgatcgcc
     1981 aaaatcgaaa aacacgaagc gctcgagcac atcgacgcga tcctcgaaac cgtcgacggc
     2041 atcatggtcg cgcgcggtga tctaggtctg gaaattgccc aggaacgaat tcccgcggtg
     2101 cagaaaatga ttattgctaa ggcgaatcgt ctgggaaagc cggtcatcac cgccacgcag
     2161 atgctgcgct caatggtgtg gaatccgcgg ccgacgcggg ccgaagtgac tgacatcgcg
     2221 aacgcgattc tggacggcac cgacgcactg atgctctcgg aagaaaccgc cgcaggtgac
     2281 tacccgctcg aagccgtcaa aacgatggcg caggttgccg aggagaccga aacgattttg
     2341 gagccgcgac aacgttttcc cgacttagaa aaaaccgttc cggaggcgat tagtctcgga
     2401 gcgatctctc tagcgcgcga gctaaaagtg aaagcgttct taattccgac cacctcgggc
     2461 agcacggcgc ggctgatcgc gcgctatcgc ccacggcagc cgatcatcgc cgtcagccca
     2521 gagcgccaaa ccgtgaagat gctttgcctg gtttggggag tacatcctgt gtccacccgc
     2581 ggcttcaaga gcacggatga aatggtacgc atgctgcagc agaaagcgct agaatccggt
     2641 ctcgtcaagc gtggcgattt agtcgcgatt actgccgggc tgccgctcca ccaaacgggc
     2701 accacgaaca tgatcacggc aaaagtgatc gaatagaaaa agcggatctt ctcaccacca
     2761 aggcacaaag cacgaaggcg tggacttgat ggtttggcgc gcttcgcgga tccggtttaa
     2821 gcgaacgctt tttctccgcc ttgcatccgt tgtcccaaac gcgctagata acattcaatt
     2881 aagcccaccg gaggtcgctc atgtccgaag cactcaaagc tttcgaagaa gagctccaga
     2941 gcaaaaactt caaaggttat tggcaaaacc agcaaggaga tgtcaatcgc gagccggtgc
     3001 cgtccttcga agcttgcttc tggaagggca aggatcttta cgccgcgatg gagaaagccg
     3061 gcgacgtggt cgggctcgac gtatcgtttc gtcgcgtcat tcagctctgg aatcccgcct
     3121 taaa
//
LOCUS       DASULZ010000760         4171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1507534, whole genome shotgun sequence.
ACCESSION   DASULZ010000760 DASULZ010000000
VERSION     DASULZ010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507534"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1011
                     /locus_tag="VFY96_15640"
     CDS             154..1011
                     /locus_tag="VFY96_15640"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX5607957.1"
                     /translation="MVPGSFEYFAPLSLPDAVKFLSEHRDDVKILSGGQSLLPLMKMR
                     LSKPAFVVDIGRLPGLDQITEEGNHLVIGALVTHAQIENSDLLETKCPLLPQTATTIA
                     DVQVRNRGTIGGSVAHADPAGDWPATVLALEGEVKIFGPNGERWVKCDEFFLGLLMSV
                     LEPDEIVTAIKVPVTGTDKTAYLKAAPRSSGFAVVGVAVRLAIDSAGNCSRAAIGITG
                     VTDKAYRPARVEQMLTGKRLDSVIIQQAAAEACRNVEAIEDINGSAEYRKQLTEVYVA
                     RAIETALST"
     gene            complement(1275..2369)
                     /locus_tag="VFY96_15645"
     CDS             complement(1275..2369)
                     /locus_tag="VFY96_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEX5607958.1"
                     /translation="MNRRDFLRHGLSVSAGVALSGLPRYAPFARANDSKWRTFEVTTR
                     IEIADPVGASRAWVPVPLMNNTDYFKREPDSWSGNYKAVRQVQTDNVGTGLVFAEWAP
                     GEKSPALEVKSRFMTQDRWVDLSQKPNPAIKENKAVLDYFRKPTKYIRTDGIVATTSK
                     GIVTGYKNDVDKARAIYEWIVDNTFRDPKVKGCGIGDITTMLESGYLGGKCADLNALY
                     VGLARAAGLPARDVYGVRVATSAEFKSLGKADDITGAQHCRAEVYLNGYGWVPVDPAD
                     VRKVVLEENLPGEQLPLEDPKVKKARAKLFGAWEMNWLPYNYSHDVKLPQSRGAEIAY
                     LMYPQGETANGRKDSLDSKSFTYSIKSKEV"
     gene            complement(2468..2935)
                     /locus_tag="VFY96_15650"
     CDS             complement(2468..2935)
                     /locus_tag="VFY96_15650"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(2801..2803),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX5607959.1"
                     /translation="MKNLDGQRAPAFSLPGNDGKKHSLDEFKDKTVVLFFYPKDSTPG
                     UTKEACGFRELSSQFKKANTVVVGVSKDSIESHSKFAEKFKLPYVLLSDPQSEVMKKY
                     GAFGKKMMYGKEVQGTIRSTVVVGPNGDVIKHWSTVKNAATHPGEVLQFLKSL"
     gene            complement(3823..4104)
                     /locus_tag="VFY96_15655"
     CDS             complement(3823..4104)
                     /locus_tag="VFY96_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEX5607960.1"
                     /translation="MKASWENTVVAESDRTIVIEGNHYFPPESIKKEFFQQSDTHSTC
                     PWKGLASYYSLRVGDNLNQDAAWYYPEPKEAAKSIKGYVAFWRGVKVTE"
BASE COUNT          929 a         1176 c         1072 g          994 t
ORIGIN      
        1 cgccgccatc ccttcacctg cggccatagc gagcgcggtg gaggacgcgt tgcagccgtt
       61 tggtgtcaaa gtttgcgatc tgccgctcac gcctgaggcc gtgtggcggc tcgctaacca
      121 caatcccgaa agcgagcgga ggtccttcta attatggttc cgggttcgtt cgaatacttc
      181 gcgccgctct cactccccga cgcagtgaag tttctctccg agcatagaga cgacgtgaaa
      241 attctttccg gcgggcagag tctgctgccg ctgatgaaaa tgcgcctgtc caagccggct
      301 ttcgtggtcg acattggccg cttgccgggg ctcgaccaga tcacggaaga aggcaatcac
      361 cttgtcatcg gcgccctggt cacgcatgcg caaatcgaaa attcggatct gctcgaaacc
      421 aagtgcccgc tactccccca aaccgcgacg actattgccg acgttcaggt tcgtaatcgc
      481 ggtaccatcg gcggcagtgt cgcccatgcc gatcccgctg gcgactggcc tgccactgtc
      541 ttggctctcg aaggagaggt caaaatcttc ggccccaatg gcgagcgttg ggtcaaatgc
      601 gatgaatttt ttctcggctt gttgatgagt gtgctcgagc cagacgagat cgtcacggca
      661 ataaaagtgc cggtgaccgg tactgacaaa acggcttatt tgaaagctgc gccgcgctcc
      721 tccggctttg ccgtggtggg cgtcgcggtc cgcctggcca tcgatagtgc gggaaattgc
      781 agccgagctg caatcgggat tactggcgtc accgataagg cttaccgccc cgcgcgagtc
      841 gagcaaatgc tcaccggaaa acggctcgac tcagtgatta tccagcaggc ggcggccgaa
      901 gcttgccgga acgtcgaagc catcgaagat atcaacggct cggctgaata ccgcaagcaa
      961 ctgactgaag tctatgtggc gcgggctatt gaaactgcat tgagtacata gaatagcaat
     1021 tcgctttgtc ttcctgccaa gctgcgtcgc tactgttttt ctaagacggc ttcgattttc
     1081 atccgcacaa actcgccgaa agtccgtttg caatcggcca agaagtcgag atgccgcacg
     1141 ggagttctat tctcccgaat aactcacatg acactcacat cgattttgtt cgattgccat
     1201 gtacagtctc aaaaacgccg aagcctccaa cgggtggagg cttcggttga acgacaaagt
     1261 ttttcaatgc tggttcaaac ttcttttgac ttaatcgagt aagtgaagct tttcgaatcg
     1321 aggctgtctt tacgtccgtt ggcagtctcg ccctgtggat acattaaata tgcgatttcc
     1381 gcaccgcggg actgcggcaa tttgacgtcg tgggaataat tgtacggtaa ccagttcatc
     1441 tcccaggcgc cgaacagttt cgcacgagct ttttttacct tcggatcttc gagcggcagc
     1501 tgctcgccag gcagattttc ttcgagaacg accttgcgca catccgccgg atcgacgggc
     1561 acccagccgt agccattcag atagacttcg gcgcggcagt gctgagctcc ggtaatgtca
     1621 tccgctttgc ctaaactctt gaattctgcg gaggtcgcta ccctcacacc gtaaacatcg
     1681 cgcgccggca atcccgccgc gcgagccaat ccaacataca gcgcgtttaa atcggcgcat
     1741 ttgccgccta aataaccgct ctccagcatg gtcgtgatat cgccgatgcc gcagcctttg
     1801 accttgggat cgcgaaaagt gttgtcgacg atccattcgt aaatcgcccg agctttatcg
     1861 acatcattct tgtaacccgt gacaattcct tttgacgtcg tggcgacaat accatcggtt
     1921 ctgatgtatt tggtcggctt gcgaaagtaa tcgagcaccg ctttgttttc tttgattgca
     1981 ggattgggtt tttgacttaa atcgacccat cggtcttggg tcatgaaccg gcttttgacc
     2041 tcgagagccg gactcttttc gcctggagcc cactcggcaa atactaaacc ggtcccgaca
     2101 ttatccgtct gcacttggcg aacggcttta tagttcccgc tccagctatc gggttcccgt
     2161 ttgaaataat ctgtgttgtt catcaacggc acaggtaccc aggctcggct ggcgcccacg
     2221 ggatcggcaa tttcgatccg agtcgtcact tcgaaagtcc gccacttcga gtcgttggcg
     2281 cgggcgaacg gcgcataccg cggcaaaccg ctgagcgcaa caccggcaga aaccgagagt
     2341 ccgtgtctaa gaaaatcacg gcgattcata gagcccccct ctgaactcaa taggaattag
     2401 caatggaaaa aatatagccc ttgggctttg aatgtcaatc gtggctcaga gatcttttgc
     2461 cgatggatca gaggctcttt agaaactgca atacttcccc gggatgggtc gcggcgttct
     2521 tgacagtcga ccagtgcttt atcacatcgc cgttcggccc aaccaccacc gttgaacgaa
     2581 tcgtgccctg cacttctttg ccgtacatca tttttttgcc gaaggcgccg tattttttca
     2641 tcacttcgct ctgggggtcg gagagcaaga catagggcag tttaaatttc tcggcaaact
     2701 tgctgtgcga ctcaatgctg tccttgctga caccgacgac cacagtattg gcctttttga
     2761 attgagaact cagctcgcgg aacccgcaag cttccttggt tcagcccggg gtactgtcct
     2821 tcggataaaa aaacagcaca accgttttat ctttgaattc atccagagaa tgtttcttgc
     2881 catcattgcc aggcaagctg aatgcgggcg ccctttgacc gtcaaggttt ttcatgcatg
     2941 tgactccttc gtctgcagtt catttcataa atgcgtaagg gagaaaaaac aaaccgccat
     3001 catgctgggc gagcaccacc gattccgctt gacgcgccat gcgtcggatc ggatcgtaat
     3061 cctcatcgac cgctggcaca aaacgctcca aaccggcccg acggaggatc gaaactccca
     3121 ttggagtttg gtgcacctca aaaagtaatc gtctcagccg tgttctgatt tcggctcata
     3181 cttttgtaga gacaacccac ggaggaaccg actcggggct atgctttgga tcacgcatat
     3241 ttgagtgaat agttcggcgc gttcgcaaat cagccactcg agaacggtgc tgtcgatcgc
     3301 ggtggcatcg accatgccgg ccaaaagcca tttcaatgag gtgcggtgag caccggacgc
     3361 aaccgccgcg tgaaaaaaac catcagtgtg ctaaaactcg gccaaataag cgcgcaccac
     3421 attgaaaccg gaatgggaga gcggctcatt gaatgcccaa cgtgcgccgc gcagttcgag
     3481 aaacaagcgg tacgcgctcg tgctcctccc aataacgtcg gagaagtaaa caggtttatg
     3541 ctgatagcgc tctcccgcgg gaggggtacc cccaatagct ctatctcatt aactcctgaa
     3601 tccgctttgt gcacgtaagg cagtccacag atccacagaa gctgaatctc acctcgatcg
     3661 aagagctgct cccgctcctg ccacgcgatt ccgtctacac actcgactgt gaccccaatt
     3721 tgtgtggcga tatacttggc gagcgcgcgg cagaacggtt cgctgttctc cgccatgcag
     3781 gaggcgagct tcaattgcgg cggagaacca aagctcaagg gtttattccg tcaccttcac
     3841 gccgcgccag aaggcgacgt atcctttgat ggatttggca gcttccttcg gctcgggata
     3901 ataccaggca gcatcctgat tcaggttgtc gccgacgcgc aagctgtagt agcttgcaag
     3961 ccctttccaa gggcaagtac tgtgggtgtc gctttgctgg aagaattctt tcttgatcga
     4021 ctcaggtgga aaataatgat tgccctcgat gacgatcgtc ctgtctgact ctgcgactac
     4081 cgtattttcc caactcgcct tcatgtcata tctccttagg atgcctcggt cacacatcga
     4141 aattttgcac agacataggg gtaatgagcc t
//
LOCUS       DASULZ010000761         3346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_594794, whole genome shotgun sequence.
ACCESSION   DASULZ010000761 DASULZ010000000
VERSION     DASULZ010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594794"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VFY96_15660"
     CDS             <1..396
                     /locus_tag="VFY96_15660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase"
                     /protein_id="HEX5607961.1"
                     /translation="RPIWSDTPLLGLLFIVSAASISAALMILLARRTVSTAAAVESLH
                     RIDAWVVALELLVLIALMITLGPVFRAWLNPWGLLLLLAIIIGMLVPLALYFRREWLG
                     ELNAKTAAVLVLLGGFLLRVVIVFSAQSV"
     gene            634..1476
                     /locus_tag="VFY96_15665"
     CDS             634..1476
                     /locus_tag="VFY96_15665"
                     /inference="COORDINATES: protein motif:HMM:NF016131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HEX5607962.1"
                     /translation="MTASESRLQDLKRRHPEWEPWLTVVQEVLRESADSRWAAVVPPI
                     AATPLGKTPLIAEARIVLPKSLLRAWFEQLIRVAQRSGTDKLATLKAALDTKLDIFKL
                     FKASLQQDDEFVKKLATSVSADAEAFAAVTALLPVPLLQATNRRWAQTVASSWMEGYC
                     PVCGAWPAFAETRGIERNRYLRCGRCGAEWQAHVLSCPYCGNTDHERLVSLISENSAK
                     SAIDACKVCVGYVKVFTTLQGTGPDAVMLEDLGSVEIDLAAADQGYRRPKGPGYSVDV
                     TVSA"
     gene            1500..1889
                     /locus_tag="VFY96_15670"
     CDS             1500..1889
                     /locus_tag="VFY96_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607963.1"
                     /translation="MSDPGKNLLPRFRPADVCRALLAALDAAEGRRRNRKRDQTPDAI
                     GLAIKREVLESVVHDDPGPELFESWLLEYVEKKESNHSPGAVSAMARAVLDEWRLAHS
                     MNDFKAWLDCGAPSEDASAGPATPRHG"
     gene            1917..2102
                     /locus_tag="VFY96_15675"
     CDS             1917..2102
                     /locus_tag="VFY96_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607964.1"
                     /translation="MPSEEVRRLLKLFGVAVTEFEDAVHQRAGPDEIRKCDAEVRSRL
                     EEIESLLERLRRRVTEQ"
     gene            complement(2109..3185)
                     /locus_tag="VFY96_15680"
     CDS             complement(2109..3185)
                     /locus_tag="VFY96_15680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX5607965.1"
                     /translation="MSLDYVSNQEIIQAARRNLPQDVWDYLTGGAESETTMRRNRFGF
                     DSLALRPRVLIDVSKIDTSTTFLGQKLRIPVMMAPIGSLQTITPEGGVAVAKAAAEFG
                     TMNFVSSVTQPTLEEIAASTNHPKIFQLYIRGGLDWCAEIIGRVKQAGYMALCLTVDT
                     AHYSRRERPLLNRWQPPSRRTETGRIFQAMLTWEMADAIKKIAGMPLIIKGIATAEDA
                     KIAVEHGVDVIYISNHGGRQLDHGRGTVETLPEIVDAAAGKAEIVLDGGITRGTDIVK
                     ALALGANAVTIGKLQGWGLGAGGKAGLVRVLELLEEELIVDMALLGVTRADQITQSYV
                     CKTQPVTIAHEMSAFVHIAGGQLR"
BASE COUNT          666 a          971 c          964 g          745 t
ORIGIN      
        1 cggccgatct ggtcagacac tcctttgctc ggacttttgt ttattgtttc ggcagcatcc
       61 atttccgcgg ccctgatgat tctgttggcg cgtagaaccg tttccaccgc ggcggcagtg
      121 gaatcgctcc accgcatcga cgcctgggtc gtcgccttgg aattattggt cttgatcgct
      181 ttgatgatca ccctcggacc cgtgtttcgc gcctggctga acccttgggg acttttgctg
      241 ttactggcaa ttattattgg catgctcgtt ccactcgcgc tttacttccg tagagaatgg
      301 ttgggcgagc tgaacgcgaa aactgccgcg gttttggtgt tgctcggcgg attccttctt
      361 cgcgtagtga tcgtgttttc tgcgcagagc gtctgaaatg aatcggtttg ttatcgcttt
      421 cctcgtcgtc atattgtctt cgtgcgtttc cgcctgctac agtcctgaga caagtcggac
      481 gcgcggcggc ggaccgggag cagacgttgg caatcgcggt ccatttgtca atatgcacga
      541 gggttcgcgc ccgttctacc aaactcccca gcttatcccg gctaagcctc cgcccctgga
      601 tagtgcccat caggcggata cgctgagccg caaatgacag cctctgagag tcgcctgcag
      661 gatctcaaac gtcgtcatcc ggagtgggag ccttggctca ccgtagttca ggaagtcttg
      721 cgcgaaagcg ccgactctag atgggcagcc gtcgtgccgc caatcgcggc gacgccgctc
      781 ggaaagactc cgctcatagc cgaagcccgc attgttctgc cgaagagttt gctgcgcgca
      841 tggtttgagc aattgatccg ggtcgcccag cggagtggaa ctgacaagct cgccaccctt
      901 aaagcagctc tcgacacaaa actcgatatt tttaaactct ttaaagcttc gcttcagcag
      961 gatgatgagt ttgtgaaaaa gctcgccaca tctgtcagtg ccgacgcgga ggcgttcgca
     1021 gcagttacgg cgttgttacc ggtcccgttg ctacaagcga cgaatcgtcg ttgggcgcag
     1081 actgtagcgt cgagctggat ggaaggatac tgtccggtct gcggtgcatg gccggcattc
     1141 gccgagacgc ggggaatcga acggaaccgt tacttgcgct gcggtcgctg cggcgcagag
     1201 tggcaagcac acgtcctctc ctgcccctat tgcggcaaca ccgatcatga aaggctcgtc
     1261 tcattgatat cagagaatag cgcgaaatcg gccatcgatg cctgtaaagt gtgcgtggga
     1321 tatgtcaaag tttttacaac gctccaaggt accgggccgg acgccgtgat gcttgaagat
     1381 ctcggcagcg tcgagatcga cttggcagcg gccgaccaag ggtaccgacg gccaaaaggg
     1441 cctggttatt cagtggacgt gactgtctcc gcgtgacccc acggcgaagc tatcagccca
     1501 tgtccgatcc tggtaagaac ttactgccac gctttcgccc cgcggatgtc tgcagggctt
     1561 tgctcgcggc cctcgatgct gccgaaggac gcagaaggaa ccgcaaacgc gatcaaacgc
     1621 ccgacgccat cgggcttgcc atcaaacggg aagttctcga atccgtcgtg cacgacgacc
     1681 ctggcccgga actattcgag agttggcttc tcgagtatgt cgagaaaaaa gagagcaacc
     1741 attcgccggg cgcggtttca gccatggccc gtgcggttct cgacgagtgg cggctggcgc
     1801 actcgatgaa tgatttcaaa gcttggctgg attgcggcgc gccgagtgag gatgcgagcg
     1861 caggaccggc cacgccgcgt catggatgag agacagaggt accgagggtt caaccgatgc
     1921 ccagtgaaga agttcgccgc ctgctgaagc tcttcggcgt tgccgttacc gaattcgaag
     1981 acgccgtgca tcagcgcgca ggaccggatg agattcgcaa atgcgacgct gaggtgcgct
     2041 cccgtctcga ggaaatcgaa tcattgctcg agcgcttacg tcgaagagta accgaacaat
     2101 agattacgtc agcgcagctg cccgccggcg atgtgcacga acgcgctcat ctcgtgcgcg
     2161 atagtgaccg gttgcgtctt gcagacatag ctctgggtaa tctgatcggc tcgggtaaca
     2221 cccaggagcg ccatatcgac gatcaattct tcctcgagta gttctagcac ccgcaccagc
     2281 ccggcctttc caccagcgcc caagccccat ccctgcagtt ttccgatggt caccgcgttc
     2341 gcgccgagcg ccaatgcttt gacgatgtct gtgccgcggg tgatgccgcc gtcgagcaca
     2401 atttccgcct tgcccgctgc ggcgtcaacg atctccggca gagtctcgac ggtgcctctg
     2461 ccgtgatcca gctggcgtcc gccatgattc gagatgtaaa tgacgtcgac gccgtgttcc
     2521 acggcaattt tcgcgtcttc cgccgtcgca atacctttga tgatcagcgg catgccggca
     2581 atttttttaa tggcgtcggc catttcccac gtgagcatcg cttgaaagat tcgcccagtt
     2641 tcggttcttc ggctcggcgg ctgccagcga ttgagcagcg gccgctcacg ccggctgtag
     2701 tgcgccgtgt cgacggtaag gcatagcgcc atgtaaccgg cttgcttcac ccgtccgatg
     2761 atttccgcgc accaatcgag cccgccgcga atgtagagtt gaaagatctt gggatgattg
     2821 gtgctggcgg caatctcttc cagagttggt tgggtcaccg agctgacaaa gttcatcgtg
     2881 ccaaattccg ctgcggcctt cgcgacggca acgccgcctt ctggagtgat cgtctgcagg
     2941 gagccgatcg gcgccatcat caccggaatg cgcaacttct gacccaaaaa cgtcgtcgag
     3001 gtatcgatct tcgaaacatc gatcagcaca cgggggcgca gggcgaggct atcgaagccg
     3061 aaacgattgc gccgcatcgt ggtttcggac tcggcgccac cggtgagata atcccataca
     3121 tcctgcggca aattgcggcg cgccgcttgg atgatctctt ggttgctgac gtaatcgaga
     3181 ctcatgaata cggctccttc gtaaactgcg atggtggcag catgatcgat tatcgcgcgc
     3241 acattatcga aacgtgcggc aaggtgtcaa cctccgaaaa aatacctgtc agatttgctt
     3301 tgctactaca cctcgaaaaa acaccgcctt tgaagtaggt cgttcc
//
LOCUS       DASULZ010000762         4873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_188460, whole genome shotgun sequence.
ACCESSION   DASULZ010000762 DASULZ010000000
VERSION     DASULZ010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188460"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VFY96_15685"
     CDS             <1..837
                     /locus_tag="VFY96_15685"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607966.1"
                     /translation="YAIEFDPVFIGASSALFNAMLSGAADLAGSGGPAVISNILTGGD
                     VIHITAMVPRFTQSIMVKSEIKKPEDMAGKKIGVSRIGTVTHFALQTLLDANGIKNVT
                     ILQMGGQPEAFAGLSRGSVDGAVFSPPYNFQLKKQGYNELASPLDLAKLTPFITNGIV
                     ARRSVAEKDKEIVIKTIKGTAEAIKLIQTDREFTKKVMSKWMPMKDADLVEQSYRFAT
                     ENYSKEGHVPEAALRGMVKQMVQSNLIDAKAAASTPMTAYYDNRYVDEVKQSGFFDQL
                     WR"
     gene            1115..1399
                     /locus_tag="VFY96_15690"
     CDS             1115..1399
                     /locus_tag="VFY96_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX5607967.1"
                     /translation="MLSAIVLLNTERDKTNKVAEMLADTDGVTEVYSVAGRYDLAAIV
                     RTSDNDQLAKIVTERIRAVPGITSSETLIAFRVYSRHDLERMFSIGMENV"
     gene            complement(1551..2606)
                     /locus_tag="VFY96_15695"
     CDS             complement(1551..2606)
                     /locus_tag="VFY96_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5607968.1"
                     /translation="MPDLIARDIQKQLGTAQVLCGVSFELQPGEVLALLGPSGSGKTT
                     LLRSVAGLERPDKGKIEIRGKVVFDDATGFEVPAEQRGLGLVFQSYAIWPHRSVFENV
                     AYGLRLRHVANAEVHQKVGDVLKRLGLDHLAERYAHQLSGGQQQRVALARAIVYNPPV
                     LLLDEPLSNLDAKLREDARAWLRELIKQIGNSALYVTHDQTEALAIADRVLLLDKGQI
                     QQEGPPEQIYGEPTSRFSAEFIGSNNCFEGKIAEVSGRRARLAGDGWTLWGTLKAEKS
                     RDAIGTGVVRVESVRLADGAGDHRLKMRLKSALYLGERWEYVLELGDLRLRTWGETSR
                     PAGEYWVSIPPDALWIF"
     gene            complement(2599..4266)
                     /locus_tag="VFY96_15700"
     CDS             complement(2599..4266)
                     /locus_tag="VFY96_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX5607969.1"
                     /translation="MNRTFRSFAVVAVALVVFGPLGLIIYQSFLSEAFFSPTARLSLG
                     AYRYVLADSGFHSAFGTSLFVSFGMTAIAVPLGSLLAFLLVRTDLPWKRFLEPWLLVP
                     IFLSPVVLAFGYVVAVGPVGFFSLWAKSLLGTIPWRLYSLYGLVVIAGLTHVPHSFLY
                     VSSALRSLDPSVEEAARVTGAGPWRIAATVSLPLVLPSILFSSALIFLLGFELFGLPL
                     VLGDPAGLLVLTTYLYKLTNLLGVPSYHLMAVVALSIVALTFPLVYAQRRMLRTAGRF
                     AVIGGKGQAFRPLPLGALRWPAVGIVLLWLIVTVLLPLIGVALRSVVSQWGEGINIAD
                     VLTLDHFRELQNHPNLVRGIINTLLLGGIGGALSVAFYAVIGLAAHRWQSRWVSFLDY
                     LVMLPRALPGLIVGLAFLWVFLFFKPIAPLRTTLFSLWIAYTVVWLAYGLRLISASLL
                     QVKPELEEAARVIGARQGKIYRDVTLPLIRFGLIGSWLLMFMMFVREYSTGVYLLGPG
                     TEVIGSLIVSLWGTGALDLISALSVIEVVSVGLILFVALRLGVRLDA"
     gene            complement(4271..>4873)
                     /locus_tag="VFY96_15705"
     CDS             complement(4271..>4873)
                     /locus_tag="VFY96_15705"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607970.1"
                     /translation="HADLVKLLKAKPDVYKEKIATFDPEKSGVGFLMMTQDAKVFPQF
                     WDLAKGLGATGAKVYTSTGAMLEKVGSGEHTLAYNIIGSYVLLRQKKDASLGYVLPKD
                     YTLVFSRIAFIPKSARNPNAAKLFLDYLLSKRGQTVMSEKSLVYSIRSDVPGPVTAAG
                     LNKDLGNAIKPIEVNTDILAGLEQNKRLDFMKQWKEALKR"
BASE COUNT         1213 a         1360 c         1350 g          950 t
ORIGIN      
        1 tacgcgatcg aattcgatcc ggttttcatt ggcgcatctt cggcgctttt caatgccatg
       61 ctgtcgggag cggccgattt ggcgggctct ggcgggccgg cggtgatctc caacatttta
      121 acgggcggcg acgttatcca catcaccgcg atggtaccac gctttacaca atcgattatg
      181 gtgaaatccg agatcaaaaa acccgaggac atggcgggca agaagatcgg tgtcagccgg
      241 attggcaccg tgacccattt tgccttgcaa actttgctcg atgctaacgg catcaagaat
      301 gtgacgattc tccaaatggg cggtcagccc gaggcattcg cagggttgag tcgtgggtcg
      361 gtcgacggcg cggtattttc gccgccctac aacttccaac tcaagaagca gggctacaat
      421 gagctggctt cgccattgga tctcgcgaag ctcacgccgt ttatcaccaa tggcatcgtt
      481 gcgcgccgct cggttgcgga aaaggacaag gaaatagtga tcaagacgat caaaggcacg
      541 gcggaagcga tcaagctcat ccaaacggac cgagaattca ccaaaaaagt gatgagcaag
      601 tggatgccga tgaaggatgc cgatctggtg gaacaatcat accgcttcgc cactgagaat
      661 tattccaagg aaggccatgt cccggaagcc gcgctccgcg gcatggtcaa gcagatggtg
      721 cagagcaact tgatcgatgc caaagccgcc gcgagcacgc cgatgacggc ttattatgac
      781 aaccgctacg tcgatgaggt gaaacagtca gggttcttcg atcagctgtg gcgataaacg
      841 agcttagttc gaaacgttca agtcgttcaa atggttcaat tcgtttgaga aatggttatc
      901 ggtaaagctt gtcgataaaa ccttccttgt cgagctccgt gacgacggaa acgtcgatca
      961 cgtcttctac ttttaacgtc gagccttcct ctggtccgag tattctccgt caactgcatc
     1021 atcaagccag gtgagcaaca ttgacgatac ggcacccctc ggctagtctt ctttagttcg
     1081 aacctcgcgg ctaatcttaa tcaaggagcc aaacatgctt tccgccattg tcctgctgaa
     1141 taccgaaaga gataaaacca acaaggtggc tgagatgctc gccgataccg acggtgtgac
     1201 ggaagtttat tcggtcgcgg ggcgctatga tctcgcggcg atcgtgcgga cgagcgataa
     1261 cgatcaactg gccaaaattg tgaccgaacg gattcgtgcg gtaccgggga ttaccagctc
     1321 tgagaccttg atcgcctttc gcgtttattc acggcatgac ctggagcgca tgttttccat
     1381 cggcatggaa aacgtgtagc agattaaact gatgcctgtt ctggtggcgc gactctaccg
     1441 cgtcatgctt aggccaagcc gaagaatctg gcgttgtatc tgctcacaaa tcgagatgct
     1501 tcgctggcgc tcagcatgac accacgacgt gtttttcgat tctcacacga ctagaaaatc
     1561 cacagcgcgt ccggagggat ggagacccag tactcgccgg cgggtcggga cgtttcgccc
     1621 caagttcgta gccgcaaatc tcccagttcg agaacgtatt cccagcgctc tcccaggtag
     1681 agagcagatt taagtcgcat cttaagacga tggtctcccg cgccgtcggc taaccgaacc
     1741 gattcaaccc gaaccacccc cgtgccgatc gcatcccggc ttttttccgc tttgagtgtg
     1801 ccccaaagcg tccagccatc gccggcgagg cgggcgcggc ggccggagac ctcggcgatt
     1861 ttcccctcga agcaattatt gctgccaatg aactcggcac taaaccggct ggttggctct
     1921 ccgtaaattt gctctggcgg gccttcctgc tggatctgtc ctttgtccag cagcaatacc
     1981 cgatcggcaa ttgcaagcgc ctcggtctga tcgtgcgtca cataaagcgc gctgttgccg
     2041 atctgtttga tcaactcgcg cagccaggcg cgcgcatctt cacggagttt ggcgtcgaga
     2101 ttcgacagcg gttcgtcgag caacaacacg ggagggttgt aaacaatcgc acgggcgagc
     2161 gcgacccgct gctgctgtcc accggagagt tgatgcgcat agcgctcggc caagtgatcg
     2221 aggccgagcc gcttcaaaac gtcaccgact ttctggtgga cctccgcgtt ggccacgtgc
     2281 cgcaggcgta acccataggc gacgttttcg aaaacactgc gatgcggcca aatcgcataa
     2341 gattgaaata ccaggccgag gccacgctgt tcggcaggaa cttcgaatcc cgttgcgtcg
     2401 tcgaaaacga ctttaccgcg gatttcaatt ttgcctttgt caggccgttc aagcccggcg
     2461 accgaacgca gcagggtcgt tttgccgctg cccgaagggc cgaggagggc gagcacttcg
     2521 ccgggctgaa gctcgaagga aacaccacat aggacttggg ccgtgccgag ctgcttctgg
     2581 atatctctag cgatcaggtc aggcatcgag tcgtacaccc aagcgtaacg cgacgaacaa
     2641 gatgagcccg accgaaacga cttcgatcac cgaaagcgcc gagatcaaat ccaatgcccc
     2701 tgtgccccag agcgaaacga tcagcgagcc gatcacttcc gtgcctggcc caagcagata
     2761 aacgccggtg gaatactcgc gcacaaacat cataaacatc aatagccagc taccgatcag
     2821 gccgaagcga atcagcggca acgtgacatc gcggtaaatt ttcccttgac gggcgccgat
     2881 gacgcgcgct gcttcttcga gctcgggttt cacttgcaac aagctggcgg agatcaaacg
     2941 tagtccataa gcgagccaca ccacggtgta ggcgatccat agactaaata aagttgtgcg
     3001 cagcggcgcg atcggtttga aaaataaaaa cacccagaga aaagcgagac cgacgatcag
     3061 acccggcagt gcgcgcggca gcatcaccaa gtaatccaag aacgaaaccc agcggctctg
     3121 ccagcgatgt gcggcgagac cgatgacggc ataaaacgcc acggacaagg cgccgccgat
     3181 gccgccgagc agaagcgtat tgataatgcc gcgaaccaag ttgggatggt tttggagctc
     3241 gcgaaaatga tccaaggtca gcacatcggc aatgttaatt ccctcgcccc attggctaac
     3301 caccgagcgc aaggctacgc cgatcagcgg cagcaatacg gtgacgatca accaaagcaa
     3361 aacgattccc accgccggcc aacgaagcgc gcctaaaggc agtggccgaa acgcttggcc
     3421 tttgccgccg atcaccgcaa aacgaccggc ggtgcgcagc atgcgccgct gcgcgtatac
     3481 caaaggaaaa gtcaacgcga caatcgataa agcgacgacc gccatgaggt ggtaagaagg
     3541 cacgccgagg agattggtaa gtttataaag gtacgttgtg agcacgagca agcccgcagg
     3601 atcgcctaac acgaggggca gaccaaacaa ttcaaagccc aataggaaaa tcagtgcgct
     3661 gctgaacagg atcgacggta gcaccaatgg caggctaaca gtggcggcaa tccgccacgg
     3721 gccagcaccg gtgacccgcg ctgcttcttc gacagacggg tccaaactcc gcagcgccga
     3781 ggaaacataa agaaatgaat gcggcacatg agtgagcccg gcgatcacaa ccaaaccgta
     3841 aagcgaatag agacgccaag ggatggtgcc gagaagcgat ttggcccaga gagagaagaa
     3901 tcccaccggc ccgaccgcaa cgacatagcc gaatgcgagg acaaccggcg aaagaaaaat
     3961 cggcacgagc agccagggtt cgagaaatcg cttccatggc aaatccgtgc gtacaagaag
     4021 aaaggccaac aagctcccca gcggcacggc aattgcggtc atgccgaagg agacgaaaag
     4081 agatgtgccg aacgccgaat gaaagccgga atcggctaaa acgtatcggt acgcgccgag
     4141 gctgaggcgc gcagtcggac tgaaaaaagc ttcgctcagg aagctctgat aaatgatcaa
     4201 ccccagcggt ccgaagacca ccagggcgac ggccacaacc gcgaacgaac gaaaagttcg
     4261 attcacgggt ttatcttttt agcgcttcct tccactgttt catgaagtcg agacgtttgt
     4321 tctgttcgag gccggcgaga atgtccgtgt tcacttcgat cggtttgatc gcgttgccga
     4381 gatccttgtt caagcctgcg gcggtgaccg gtccgggtac gtcactgcgg atcgaataga
     4441 ccaacgactt ttccgacatc actgtttggc cgcgcttgga gagcagataa tcgagaaaga
     4501 gcttcgccgc attgggattg cgcgcgcttt tcggaataaa ggcgatgcga gaaaatacca
     4561 aggtgtaatc tttcggaaga acatagccaa gtgaagcgtc ttttttctgt cgcaagagca
     4621 cgtacgagcc gatgatgttg tatgccagtg tatgctcgcc ggagccaacc ttctccaaca
     4681 tggcgccggt gctcgtgtaa accttcgccc ccgtggcacc gagtcctttg gcgagatccc
     4741 agaactgggg aaagaccttt gcgtcttggg tcatcatgag aaacccaacg ccgctctttt
     4801 ccgggtcgaa cgtcgcaatt ttttccttgt agacatccgg tttcgctttc agcagcttga
     4861 ccagatccgc atg
//
LOCUS       DASULZ010000763         3641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_797349, whole genome shotgun sequence.
ACCESSION   DASULZ010000763 DASULZ010000000
VERSION     DASULZ010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797349"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VFY96_15710"
     CDS             complement(<1..432)
                     /locus_tag="VFY96_15710"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEX5607971.1"
                     /translation="MIVDVAIVAVKAFLVLFMVLNLAGVLGWVERKGSALIQDRIGAN
                     RASIFGFAGMGLVNTLIADPLKFLTKEDFIPAAGDKLLHTLAPCMALFPALVTFAVIP
                     FGDVLILGDRVINLQVANLNVGILYVFAMGSLGVYGIVIGAW"
     gene            complement(429..2015)
                     /locus_tag="VFY96_15715"
     CDS             complement(429..2015)
                     /locus_tag="VFY96_15715"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5607972.1"
                     /translation="MDPIKITIDGKEVITSKGKTVIQVADELGVTIPRYCYHPKLSIA
                     GNCRMCLVEIEKMPKLQIACNTQVAEGMSVLTQSPKVLAVRKAVLEFLLINHPVDCPI
                     CDQAGECWLQDYYMEHDRQESRFEVGKVHDRKRTIFGPNVVFDGERCIKCTRCVRFCQ
                     EITKTNELTVVNRSDHSTIALFDDSVLDNPLSANVVDICPVGALTDRDFRFKVRVWYL
                     QKTPSICPGCSTGCNISVETYQNRIARFKPRVNEAVNSHWLCDEGRYCFHDLVGGERL
                     TTPLIRQEGGLVAATWEQALQAVYAGLKGSPSLAAILSGRNTNEEAYLFARLMKKLRA
                     DAALEVFYQERELNEVEKILVSPDRSPNFRGARDMGVGSSDGFDGLLRRLLFGDFAAA
                     FVVGEDLTKAADPETVRAALQKLSFLVVQDTLVTETSKLAHVVLPATHFGEKEGTYTN
                     RKGRLQKLNAALIPPEGALPDSEIFARLLELAGEQNGYGTPGRIFAALAQEIPAYEKL
                     TYDSIGAQGIELGSGGGGAS"
     gene            complement(2102..3370)
                     /gene="nuoF"
                     /locus_tag="VFY96_15720"
     CDS             complement(2102..3370)
                     /gene="nuoF"
                     /locus_tag="VFY96_15720"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX5607973.1"
                     /translation="MEKILLRNIDVPDFHRLATYESAGGYRSWRRVVENMKPAELIDE
                     VKASGLRGRGGAGFPTGMKWSFVPKDSPKPKYLVCNADESEPGTFKDRLLIERDPHAI
                     LEGTLIAAYAIQSHTAFIYIRGELAFGATRLENAVAEAYQAGIIGKNILGSGYDLDVI
                     VHRGAGAYICGEETALLSSLEGSRGWPKVKPPFPATHGLFGCPTVVNNVETLAALPWI
                     LERGAAAYASMGTEKSKGTKLFSVSGHIAKPGVYEVEMGYPFKKFLEEDCGGVPNGKK
                     LKGVIPGGGSMPVLRPEEIEKVNLDYESVQAAGSLLGSGGVIVMDETTCMVRAAWNLA
                     RFFAHESCGQCSPCREGCHWMEKIFHRIEHGEGESADLDLILNISGNIMGNTICPFGD
                     AAAMPAAAFIKKFRDEFDAHIAEKKCTFAN"
     gene            complement(3429..>3641)
                     /locus_tag="VFY96_15725"
     CDS             complement(3429..>3641)
                     /locus_tag="VFY96_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX5607974.1"
                     /translation="CALGGAERITAFIKRKLGIDIGQTTADGRFTLSEVECLASCGTA
                     PMLQINDDYYENLNEEKTEKILDSLK"
BASE COUNT          795 a         1078 c          977 g          791 t
ORIGIN      
        1 ccaggcgccg atgacgatgc cgtaaacgcc gagagatccc atggcgaata cgtaaagaat
       61 gccgacgttg agattggcga cctgcaaatt gatcactcga tcgccgagaa tcaatacgtc
      121 gccgaagggg ataacggcaa aagtcaccag cgcgggaaag agcgccatgc acggggctaa
      181 ggtgtgcagc agcttatcac cggcggcggg gatgaaatct tccttggtga ggaatttaag
      241 cggatccgca atcagggtgt tcaccaaccc cattccggca aaaccaaaaa tggaggcgcg
      301 gttggcgccg atgcgatcct ggatgagcgc gctgcctttc cgttccaccc aacccaaaac
      361 cccggccagg ttgagaacca tgaacaaaac gagaaaggct tttacggcaa caatggcaac
      421 gtcaacgatc atgatgctcc gccaccacca ctgcccaact caatcccctg agcgccgatc
      481 gaatcatagg ttagtttttc gtaggcggga atttcctgag ccaacgcagc gaagatccgt
      541 cccggtgtcc cgtagccgtt ttgttcgcca gcaagttcca acaaccgcgc aaagatttcg
      601 ctgtctggga gcgcgccttc gggcgggatc agcgcagcat tgagtttttg cagtcgtccc
      661 ttgcgattgg tgtaagtccc ttccttttcg ccgaagtgcg tcgccggcag aacaacgtgc
      721 gcgagcttgc tggtttccgt cacaagcgta tcctgaacca ccagaaacga gagtttttgc
      781 aaggcggcgc gaaccgtttc cggatccgca gcttttgtca ggtcttcgcc gacgacaaac
      841 gcggcggcaa aatcgccgaa cagaagccgg cgcagcaacc cgtcaaagcc gtcgctcgag
      901 cccactccca tatctcttgc accgcggaaa ttcggcgaac gatcgggact gacgagaatc
      961 ttttctacct cgttcagttc gcgctcctgg tagaaaacct cgagcgctgc atcggcgcgg
     1021 agcttcttca tcaggcgggc gaaaagataa gcttcctcat tggtgtttcg cccggaaaga
     1081 atcgccgcaa gcgaagggga tcctttgagt cctgcgtaga ctgcttgcaa cgcctgctcc
     1141 catgtcgcgg ccactaagcc gccctcctga cgaatgagcg gcgtggtcag gcgttcgccg
     1201 ccgacgagat cgtgaaagca ataacgacct tcgtcacaca gccaatggct gttgaccgct
     1261 tcattgacgc gcggtttgaa acgggcgatg cgattttggt acgtctccac gctgatgttg
     1321 catccggtgc tgcagccggg acaaatcgac ggcgtctttt gcaaatacca gacgcgcact
     1381 ttgaaacgga aatcccggtc cgtgagcgcg cccaccggac agatatccac cacgttcgcg
     1441 gaaagcgggt tatcgagcac gctatcgtcg aagagcgcga tcgtcgaatg atcgctgcgg
     1501 ttgacgacgg tcagttcatt ggtcttggta atctcctggc agaagcgcac gcagcgcgtg
     1561 cacttgatgc agcgctcacc gtcgaaaaca acgttgggtc cgaatatcgt tctcttgcga
     1621 tcgtgaactt tcccgacctc gaaccgactt tcttggcgat cgtgctccat atagtagtct
     1681 tgcagccagc actcgcccgc ttgatcgcag atgggacaat ccactggatg attgatcagt
     1741 aaaaactcca gcaccgcttt gcgcaccgcc aagactttag ggctttgcgt caaaacgctc
     1801 atgccctccg ccacctgggt gttgcaggcg atctggagct tgggcatctt ctcgatttcc
     1861 accaggcaca tccggcaatt gccggcgatg gagagcttgg ggtgataaca ataacgcggg
     1921 atcgtgacgc cgagctcgtc ggccacttga atcaccgtct tgcccttgct ggtgatgact
     1981 tccttaccgt cgatggtaat ttttatcgga tccatgttta aaaaatcgca tatctgcgct
     2041 ggatgataga agaacgcctt cggggagtgc cctcatcgat cttctatctt cgacctcgtg
     2101 ttcaatttgc gaacgtgcat ttcttctctg cgatgtgcgc gtcgaactca tcgcggaatt
     2161 ttttgataaa cgccgccgcc ggcatcgccg ccgcgtcccc gaaggggcag atcgtatttc
     2221 ccataatgtt gccgctgatg ttgagaatca aatccaaatc cgccgattcc ccttcaccgt
     2281 gttcgatccg atgaaaaatc ttttccatcc agtgacaacc ttcgcggcac ggactgcatt
     2341 ggccgcacga ctcatgagcg aaaaaacgag cgagattcca ggcagcacgg accatgcaag
     2401 tggtctcgtc catgacgatc acgccgcccg agccgagcag actgccagcg gcttgaacgg
     2461 attcgtaatc caagtttact ttttcgatct cctcgggccg gagcaccggc atcgagccgc
     2521 cgcccggaat aacccccttg agctttttgc cgttaggaac cccgccgcag tcttcctcga
     2581 gaaatttctt gaacggataa cccatctcga cttcatacac acccggcttg gcgatgtgac
     2641 cgctgacgct aaaaagtttc gtccctttgc ttttttccgt acccatcgag gcataggctg
     2701 cggcgccgcg ctcgagaatc cagggtaggg ccgccagcgt ctcgacgttg ttcacgaccg
     2761 ttggacagcc aaacagaccg tgagtcgccg ggaacggcgg cttcactttg ggccagccgc
     2821 ggcttccctc cagagacgag agcaaggccg tctcctcgcc acaaatgtag gcacccgcgc
     2881 cgcgatggac gatgacgtcg agatcgtaac ccgagcctaa aatatttttg ccaatgatgc
     2941 cggcttgata cgcctctgcc accgcgttct ccagccgcgt cgcgccgaag gcgagctcac
     3001 cgcgaatata gataaaagcg gtgtgcgatt gaatcgcata ggcggcgatc agagttccct
     3061 cgaggatcgc atggggatcg cgctcgatca gtaatcggtc cttgaaagtc cctggctcgc
     3121 tctcgtcagc attgcagacc aggtatttcg gcttcgggct atccttcggc acaaagctcc
     3181 acttcatgcc ggtgggaaaa cccgcgccac cacggccgcg cagcccggaa gctttgactt
     3241 cgtcgatcag ctccgccggt ttcatattct cgacgacccg gcgccacgat cggtaacccc
     3301 cggcggactc ataggttgcc agccggtgaa agtcgggaac atcgatattg cggagaagaa
     3361 ttttttccat tttttttgat acgactcgca gcgaacaaag cggtccgctg ccgcagcagt
     3421 gccaaaactc actttaacga atcaagaatt ttctcggtct tttcttcgtt cagattttcg
     3481 tagtagtcat cattgatctg cagcatcggg gccgtgccgc aggaggcgag acattcgacc
     3541 tcgctcaagg taaaccggcc atcggccgtg gtttgcccga tatcgatgcc gagttttcgc
     3601 ttgatgaagg cagtgatgcg ttccgctccg ccgagcgcgc a
//
LOCUS       DASULZ010000764         3342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1000706, whole genome shotgun sequence.
ACCESSION   DASULZ010000764 DASULZ010000000
VERSION     DASULZ010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000706"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VFY96_15730"
     CDS             <1..434
                     /locus_tag="VFY96_15730"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5607975.1"
                     /translation="PAAERVIAPVSELAQRLDLEVILMHVTKHVYSGPPDAFLPVFGA
                     IPNLKELWEHDTALGAAYLTEKVTELRGRGVSRVSSRVLSGGVDGAAGEIIAAAENTP
                     GNLIAITSVGESSLGRWLVGSVTERVVCHSRTPVLVTRPQA"
     gene            437..1756
                     /locus_tag="VFY96_15735"
     CDS             437..1756
                     /locus_tag="VFY96_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5607976.1"
                     /translation="MVGTGGESDAGERESEYQRVINAWAMYDWANSAFAVVMVTAIFP
                     VYYRELVLNAGGAPADATAYWAYTNSFSLLLIALIGPLLGATADAIGGKKLFLSIALF
                     IGVLGSAALAWVRNDNFILASLIFSVANLGFAGGNIFYEALLPHIARADDLDRVSARG
                     YAFGYLGGGILLIIDVLSLLRPDWFGMPDRNFALRACFVSVALWWAVFALPLLRARTA
                     RESIQRIGVGAIVSGFVRLRATFGQVRRYQQMALFLVAFWIYNDGISTIIKLAAAYGD
                     EIGIRHDDMLVALVVTQLVGFPSSFAFGTIATRLGAKRAILAGLAAYALISIAGFLIR
                     SAWHFFVLAIVVGLVQGGTQALSRSLFASMVPKTRSSEFFGFFSTGEKVAGIIGPAIF
                     GIIGQLTGSSRWGIVSVVVLFVAGALILMRVDVSEAQRVAEAADAST"
     gene            complement(2434..2964)
                     /locus_tag="VFY96_15740"
     CDS             complement(2434..2964)
                     /locus_tag="VFY96_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607977.1"
                     /translation="MDPKLLFVDRLLQLERRIGRIYQKWAAHENFPADLRSFCKAMAD
                     DEQQHLTILERSAGLLNFASTPPASAATRLKNVETAIAEAERFSDRPDLEIDGLLSRA
                     LALESSELNQLDDTWLASFQPDLGKLTQGWAHAHEEHLHRLYDATSRFTANENLYKQA
                     AALLSRYELEAAAHGT"
BASE COUNT          655 a          871 c          966 g          850 t
ORIGIN      
        1 cacccgcggc tgaacgggtg atcgcgccgg tgtcagaact ggctcagcga ttggatcttg
       61 aagttatatt gatgcacgtg accaagcacg tctacagcgg accgccggat gcttttctgc
      121 cggtgtttgg cgcgattccc aatttgaaag aattgtggga gcacgatacg gcgctcggag
      181 ccgcgtactt gaccgagaag gtcacggagt tgcgcgggcg cggagtgagc cgcgtctcga
      241 gccgcgtgct gtcgggtggc gtcgacggcg ctgccggtga aatcatcgcc gccgccgaaa
      301 acacacccgg aaaccttatt gccatcacga gcgttggcga aagcagcctc ggacgctggc
      361 tcgtcggcag tgtaacagaa cgagttgttt gtcactccag aactccggtg ctggtcacac
      421 gtccgcaagc ttaagcatgg tcggtacagg cggggagagt gacgccgggg aacgagagag
      481 cgaataccaa cgcgtaatca atgcatgggc tatgtacgac tgggcgaatt ccgcgttcgc
      541 cgtggtcatg gtcaccgcca tttttccggt ttattaccgg gaattggtgc tgaacgctgg
      601 cggcgcgccg gccgatgcca ccgcatattg ggcctatacc aactcgttct cgttgctgct
      661 gatcgcactg atcggcccgt tgctgggcgc gacggcagat gccatcggcg gcaagaagct
      721 attcctctcg atcgcgctgt ttattggcgt tctcggttca gcggcgctgg catgggttcg
      781 aaacgataat ttcatcctcg cttcgcttat tttttccgtt gccaatctcg gatttgccgg
      841 cggcaacata ttttacgaag cgctattacc gcacatcgcg cgagcggatg atctcgatcg
      901 cgtttcagca cgtgggtacg cgttcggcta tcttggcggc ggcatattgt tgattatcga
      961 tgttctgtcg cttctgcgac cggattggtt tggtatgcct gaccgaaatt ttgcgctgcg
     1021 tgcttgtttc gtgagtgtag cgctgtggtg ggctgttttc gctctgccgt tgctgcgcgc
     1081 ccgcaccgcc cgggagagca ttcaaagaat cggcgtcggg gcgattgtca gcgggtttgt
     1141 tcggctacgc gcaactttcg gacaagttcg ccgctaccag cagatggcgc tctttctcgt
     1201 ggcgttttgg atctacaacg atggtatcag cacgattatc aaactcgctg ccgcatacgg
     1261 cgacgaaatc ggcatccgtc atgacgacat gctcgtggcg ctcgttgtga cgcagttggt
     1321 tgggtttcca agctccttcg ccttcggcac catcgcgact cggctcggcg ccaagcgcgc
     1381 catcctcgcg ggtttggccg cctacgcact gatttcaatc gccggattct tgatccgcag
     1441 tgcatggcac ttcttcgtgc tggccatcgt agttggattg gtgcaaggag gaacgcaggc
     1501 gttgagccgt tcgctttttg catcgatggt gccgaaaacc cggagcagcg agtttttcgg
     1561 cttcttcagc accggggaga aggtcgccgg aattattggt ccagcgattt ttggcatcat
     1621 tggtcagttg acgggcagca gtcggtgggg aattgtttcg gtggtcgtgc tattcgtcgc
     1681 cggcgcgctc attttgatgc gcgtcgatgt ctcggaagcc cagcgcgtag cagaagcagc
     1741 ggacgcctcg acgtaaacga aattcactgt cattccgggc aagctggcgt tagcaagcgc
     1801 gaccggaatc caaaaatctc acagcgttgc ggcgagggaa tcacagatct tggagcttca
     1861 gattggcgga cgattgctcg attgctcgtt cttttcctgg aatcgcttca gtgtttcttc
     1921 tagtttttga atctctgccg attccgcaat cggcacggct tacccttcgc agctcgaagc
     1981 gggtgatgca ccgcatcgag aagaccgggt actcgggaaa aatcagcgtg caagatgtgc
     2041 tcttccggag gcaggctcct tgcactgaac agatctaatc gcaggaatac aacagagcat
     2101 acattttctt tgatattttt tagcggagta ctgatgagcg ccatcgcgct cgtcggtagt
     2161 ctgactcttg tcctccgaga agcctcaaag cgagttttgc tacgcggctt tcggcctggg
     2221 cattcgtttt gcgtttgatg cccagaaaac gaatcgctct gatcgctcag agtccgagtg
     2281 cccgaacatg tcgagagcct tgacgtacca gaagtagaca gccttctgga ctttttcttt
     2341 ctgctcgtct cccctggcgg caagctccgt agttttgttg gtgccaaatc cgatatggcc
     2401 caactttttt ttgacgattt ccggaagaat ttgctatgtt ccgtgcgccg cagcctcgag
     2461 ctcgtatcgg gacaataagg ccgccgcctg tttgtagaga ttctcgttag cggtaaatct
     2521 ggatgtcgcg tcgtagagac gatgcagatg ttcttcatgt gcgtgtgccc agccctgggt
     2581 tagtttgccc aaatcaggtt gaaaactggc caaccaggta tcatcgagct ggtttaattc
     2641 cgaactttcc agagcaaggg cgcggctcaa cagtccgtcg atttcgagat cgggcctatc
     2701 gctgaagcgc tctgcctcgg cgatcgccgt ttctacgttc ttcagccgtg tagcagcgct
     2761 cgccggcggc gttgaagcaa aatttagcag acccgcagag cgttccaaga tcgtgaggtg
     2821 ttgttgttcg tcgtcagcca tagctttaca gaatgatcgg agatcggccg ggaagttttc
     2881 atgagccgcc catttctggt aaatgcggcc gatgcgccgt tcaagttgaa gcagacgatc
     2941 aacaaagaga agcttggggt ccatggcgaa gcatcctctc actgtgaaca gagttcgctg
     3001 caggaaatat tcagttcgat gaagcttgag ccggagctgt atttttatag atgagaatta
     3061 ctttttcact tctgaaaaat tcttaaccgc aattttgtgc ttaaatcaac acaggagccg
     3121 gatcgggata atttttcaag gcgccattcg ggtttgatgg aatcgcgctc gttccgtaaa
     3181 gcagccagat ctactttcag cactggcacg cgtcttgccc ttaaacatca tgcatctctt
     3241 gtgtgaggtc tgttcacagg aggacaaaat gcgttttgct gataagcgaa tagcatatac
     3301 tttgtgtcgc gcgcggttgg cgcagcgccg tgcggtcgtc ct
//
LOCUS       DASULZ010000765         5695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_88545, whole genome shotgun sequence.
ACCESSION   DASULZ010000765 DASULZ010000000
VERSION     DASULZ010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88545"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..2133
                     /locus_tag="VFY96_15745"
     CDS             139..2133
                     /locus_tag="VFY96_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018461474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX5607978.1"
                     /translation="MLSTTEKILFVLLAVGCLSYGAQRFYAVYRAIQRGKPDPRFEHL
                     FKRIRDALWIVLTQESVFKKRPIVSLLHALVFYGFVFYFLVNLVDVLEGFIAIDARGG
                     AWNPFNLAADLLTTAVLIGIIGLVLRRWFVKPKDFAFPPNVPLLPEVRAGIARDSSVV
                     AGFIVFHVGSRLLFKATQLAEQGTDLYQPVGSFFASALAAVAPGGLDVANHFFWWGAL
                     GSILLFIPYFPRSKHIHIFLAPINLALKKERPGALSPMDFEKEEVFGAVKLEDFTWPR
                     LLDSYACIMCNRCQDVCPATHTGKALSPAAILINERYELNKILPEFASGQASPRPLLD
                     FALNDEAAWACTTCNACIEVCPVGNEQMLHVIDVRRERVLTAAEFPKGLQNTFNHMER
                     VGNPFGISADERFVWAKDLPFPVRTIEQARDAEVLYWVGCAVAFDPRAQKIARAMAAI
                     FNAAGVNWAVLGKDEKCTGDPARRTGNEYLFTQMAEENISALKESNVKTIVTTCPHCY
                     HTIANEYPQFGGNFVVKHHTDFIDELIQSGRLKLAAESNGAVTYHDPCYLGRHNGIFE
                     QPRKLIQSAGAKLIEPERTRRNSFCCGAGGGQFWKEEEKGTERVSTNRYRELKQTGAK
                     TVATGCPFCMRMITEEAAKEEPESAMQIYDVAELVAGKIQ"
     gene            2136..2597
                     /locus_tag="VFY96_15750"
     CDS             2136..2597
                     /locus_tag="VFY96_15750"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX5607979.1"
                     /translation="MRRKFVCFLLLGLAMLRGVTACGAEPRVTIATQQGRTVAFNVEI
                     ADSPAKRELGLQYRRDLASDHGMIFLFPDQSEQTFWMKNTPIPLDMIFINRELKIVGI
                     VERAVPFSLDPRSVGAPSQYVLEINGGLAKRNGIRPGDSVSFEGIASRGHD"
     gene            complement(2640..3707)
                     /locus_tag="VFY96_15755"
     CDS             complement(2640..3707)
                     /locus_tag="VFY96_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607980.1"
                     /translation="MAQKKIVHIVGTGTIGEPLIGILSTFKEQFGIDEITFHKRTPLV
                     TDRSKVIVSCRQGAKLAVDKDRWQKFVEMGMQPTLEAEEAIERANVIIDCTPVGNENK
                     EKLYSKYAGNGRGFIAQGSEDGFGKKYARGINDRALEKGKDQFIQVVSCNTHNLAALI
                     DTIALGPEKEDNLAESRFVCMRRANDLSQEGDFIPAPEASKHDDPKFGTHQGRDAHYL
                     FKTLGLDLDIYSSALKLNTQYMHTIHFDIKLRRGITKDELMRRLKSNLRVALTNKKSS
                     ASVFSFGRDHGMFGRILNQTVVVSQSLAMRGDKEIVGTCFTPQDGNSLLSSIAATLWF
                     MYPDSYEEKLAPVRPYFFSEI"
     gene            3853..5298
                     /gene="tldD"
                     /locus_tag="VFY96_15760"
     CDS             3853..5298
                     /gene="tldD"
                     /locus_tag="VFY96_15760"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEX5607981.1"
                     /translation="MSTATPPALFFGDNFGLRNSDLEKVLDLALAKKADYADLYFEYR
                     QNEGISLEEGMVKNCSQSTANGVGVRVLAQAKTGYAYTDDITIDKLELAARTAHYIAQ
                     NQTSPVPVPIAQPRAEAHDLYPIKTPVNEVGLEKKVGLLYEIDKFARDLDARVKNVFV
                     SLGSEYKVILIASSQGKVIGDIQPLTRLNVTCIAEENGNRQIGSFGGGGRVEFGFFVD
                     ADDWQRYAREAVRQAILNLSAIDAPAGTMDVVLGPGWPGILLHEAIGHGLEGDFNRKK
                     TSAFSDRIGQKVASELCTVVDDGTIPSRRGSINVDDEGTPTHRTVLIEKGVLKGYLQD
                     RLNAGLMKMALTGNGRRESYAHIPMPRMTNTFMLAGDSGPDEIIRSVNKGLYAVSFGG
                     GQVDITSGKFVFSASEAYMIEHGKVTQPVKGATLIGNGPDVLTRVSMVGSDLKLDEGV
                     GTCGKDGQSVPVGVGLPTIKINGLTVGGTRA"
     gene            5414..>5695
                     /locus_tag="VFY96_15765"
     CDS             5414..>5695
                     /locus_tag="VFY96_15765"
                     /inference="COORDINATES: protein motif:HMM:NF013673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HEX5607982.1"
                     /translation="MERFELSQDLGSDILSKAKAKGATQGDVVMSESESFFVTVRMGE
                     VEKISQAGEKRLGLRLFFGNSSASASTSDISHQSIEKLVDDTARMAEITA"
BASE COUNT         1284 a         1468 c         1623 g         1320 t
ORIGIN      
        1 cagacacatc aactgaggcc acggtttacg catccactac cgtcaagctg ccggctcagc
       61 cacggggcga ttctcccgat attgtgacgg tttgccatcg cgcacaataa taattgagac
      121 ggaggtcgat caagcgagat gttgagcacg accgaaaaaa ttctttttgt tctgctcgca
      181 gtggggtgtc tctcatacgg cgctcaaagg ttttatgcgg tttaccgcgc gattcaacgc
      241 ggcaagcccg atccgcgctt cgagcatctt tttaagcgca ttcgggacgc gctatggatc
      301 gtcttgacgc aggaatcggt tttcaagaag cggcctatcg tttcgctcct gcacgccttg
      361 gtgttctacg gctttgtttt ttatttcctg gttaatctcg tcgatgtcct ggagggattt
      421 atcgctatcg acgcgcgcgg aggcgcgtgg aacccgttca atctggccgc cgatcttctt
      481 accacggctg ttttgatcgg catcatcggc ctggtgttgc ggcgctggtt cgttaagccg
      541 aaagacttcg cctttccgcc gaatgttccg ctcctcccgg aagtgcgcgc gggaattgct
      601 cgtgattcgt cggtggtcgc cggatttatt gtctttcacg tcggctctcg gctcctcttc
      661 aaggccacgc aactggcaga gcagggaacg gatttgtatc agccggtcgg ttcgttcttc
      721 gcgtctgcgc ttgccgctgt ggcacccggc gggttggacg tcgcgaatca ttttttttgg
      781 tggggcgcat tgggatcaat cctgctcttc atcccctatt ttccacgctc caaacacatc
      841 catatttttt tagcgccgat caatttggcg cttaaaaaag aacggccggg cgctctcagt
      901 cccatggact tcgagaagga agaagtcttt ggcgccgtca agctggagga tttcacatgg
      961 ccgcgtctat tggactccta tgcctgcatc atgtgtaatc gctgccagga cgtttgtccg
     1021 gcgacccata ccggcaaggc tttgagcccg gcggcaattc tgatcaatga gcgctacgag
     1081 ttgaacaaaa tcctgccgga atttgcttcc ggccaagcga gtccgcggcc gttgctggat
     1141 tttgcgctaa acgacgaggc cgcgtgggcg tgcacgacgt gcaacgcttg catcgaagtt
     1201 tgcccggtgg gcaatgaaca gatgctgcac gtgatcgatg tgcgtcgtga gcgagtgctt
     1261 acggccgcgg agtttcccaa ggggctacaa aacactttca accatatgga acgggtaggg
     1321 aatccctttg gtatctcggc cgatgagcgc ttcgtctggg cgaaggacct gccatttccg
     1381 gttcggacca tcgagcaggc gagggatgca gaggtgcttt attgggtggg ttgcgccgtg
     1441 gcattcgatc cgcgggctca gaagatcgcc cgcgcgatgg cggcgatctt caatgccgcc
     1501 ggcgtgaatt gggcggtgct cggaaaagac gagaaatgta ccggggatcc agcacggcgc
     1561 acaggcaatg aatatctttt cacgcaaatg gccgaagaaa atatcagcgc attgaaggaa
     1621 agtaacgtga aaacgatcgt gacgacttgc cctcattgct atcacacgat cgccaatgaa
     1681 tatccccaat tcggtggaaa ttttgtcgta aagcatcaca cagatttcat cgacgagttg
     1741 atccagagcg gtagattgaa gcttgccgcc gagtccaacg gcgcggttac ttaccatgac
     1801 ccttgttacc tgggcaggca caatgggatc ttcgaacagc cgcggaagtt gatccaatcc
     1861 gccggtgcca aactgatcga accggagcgg actcgcagga acagcttctg ctgcggagca
     1921 ggcggcgggc agttctggaa ggaagaggag aagggaaccg agcgtgtaag caccaaccgc
     1981 taccgcgagt taaagcagac aggggctaag accgtagcga ccggatgccc gttctgcatg
     2041 cgcatgatca ctgaagaagc ggcgaaggaa gaacccgaga gtgcgatgca aatctacgat
     2101 gtggcggagc tggttgccgg aaaaatccaa taggtatgcg tcgcaaattc gtttgctttt
     2161 tgttgctggg gctggccatg cttcgggggg tgactgcctg cggcgctgaa cctcgagtga
     2221 caattgctac ccagcaaggt cgaaccgttg cgtttaatgt tgaaatcgct gattcacccg
     2281 cgaagcggga gctgggattg caatatcgcc gggatctcgc ttcggatcac ggcatgatct
     2341 ttttatttcc ggaccagtca gagcagacct tctggatgaa aaacacaccg attccgttgg
     2401 atatgatatt cataaaccgc gagctgaaaa ttgtcgggat tgtcgagcgg gcagtgccgt
     2461 tttcgttgga tccacgctcc gtgggcgcgc ccagtcagta cgtcctggaa atcaacggcg
     2521 gtttagcgaa acgcaatggc attcgaccgg gagacagcgt aagttttgaa gggatcgcaa
     2581 gtcgcgggca cgattaaaaa tccgctccgt ttgatgcgga gcagagttaa tcaaggcggt
     2641 caaatctcac tgaaaaaata gggcctcacc ggcgcgagct tctcctcata agaatcggga
     2701 tacatgaacc acaaagtggc ggcaatgctg ctcaatagcg aattgccatc ctgcggcgta
     2761 aagcaggtgc caacaatttc cttgtcgccg cgcatcgcca aactctgact cacgaccacg
     2821 gtctgattga gaattcggcc aaacattcca tgatcgcggc cgaacgaaaa aaccgatgcc
     2881 gaagactttt tattggtgag ggcaacgcgc aggttgcttt tgagccggcg catcagctca
     2941 tccttggtaa tgccgcggcg cagcttgatg tcgaaatgga tcgtgtgcat gtattgcgtg
     3001 tttaacttca gcgccgaaga atagatatcg agatccagtc ctagcgtttt gaacaggtag
     3061 tgcgcgtcgc gaccctgatg tgtgccaaac ttcgggtcat cgtgcttgct cgcctcgggc
     3121 gcaggtatga aatcgccctc ctggcttaaa tcgttggcgc gacgcatgca cacaaaacgg
     3181 ctttccgcga ggttgtcttc tttttccgga ccgagtgcga tcgtgtcgat cagcgccgcc
     3241 aggttgtggg tgttgcagct gacgacttgg atgaactgat ccttgccctt ctcgagagcc
     3301 cggtcattga tgccgcgcgc atactttttg ccgaaaccat cttcgctgcc ctgtgcaata
     3361 aaaccgcggc cattgccagc gtacttgcta taaagcttct ctttattttc gtttcctacc
     3421 ggcgtgcagt cgatgatcac attggcgcgt tcgatcgcct cctccgcttc aagggttggc
     3481 tgcatgccca tctcgacgaa tttttgccaa cgatccttgt caaccgcgag tttcgcccct
     3541 tgtcgacacg aaacgataac tttcgagcga tctgttacca gcggggtgcg tttatgaaag
     3601 gtgatctcgt cgatgccgaa ctgctctttg aacgttgata aaatcccgat cagcggttca
     3661 ccgatggttc cagttccgac gatgtggacg attttttttt gtgccatggt agtccgccct
     3721 cgcaattttg atacgcgaat actagcccaa gaatatgtac cgatcaacca gtttcggcgc
     3781 cagaagcagt taaaatcaaa tggcttactt ccaaagaggc catgtggtaa tagttgggcg
     3841 atggaggaga aaatgtccac cgcaacccca cccgcgctgt ttttcggcga caactttggg
     3901 ctgagaaata gcgacttgga aaaagtgctc gatctagcat tagctaaaaa ggccgattat
     3961 gccgatctct atttcgaata ccggcaaaac gagggcatca gcctcgagga agggatggtt
     4021 aaaaattgct cccaatcgac tgccaacggc gtcggcgttc gggtattggc gcaggcgaag
     4081 acgggatatg cctataccga cgatatcacg atcgacaaat tggagctcgc cgcgcggacg
     4141 gctcattata tcgcgcaaaa ccagacgtcg ccggtacccg tgcccattgc acagccgcgc
     4201 gcggaagccc acgaccttta tccgatcaaa actccagtga atgaggtcgg gctcgaaaaa
     4261 aaggtcgggc tactttacga gatcgacaag ttcgctcgcg atctggacgc gcgagtcaag
     4321 aatgttttcg tttcactcgg gagcgaatat aaggtgatcc tgatcgcttc gtcccaagga
     4381 aaagtgatcg gcgacattca gcctctcacg cgtctaaacg ttacctgcat cgctgaagaa
     4441 aacggcaatc gccagatcgg cagttttggc ggcggcggca gagtcgagtt cggttttttc
     4501 gtcgatgccg atgattggca acgctacgcc cgggaggcgg tgcgtcaagc gattttaaat
     4561 ttaagcgcca tcgatgcgcc ggcgggaacc atggacgttg ttctggggcc aggctggccg
     4621 ggcattctat tgcacgaagc gatcggccac ggtctcgaag gtgatttcaa ccgcaaaaag
     4681 acttccgcct tctcggaccg gatagggcaa aaagttgcat ccgagctctg taccgtggtc
     4741 gatgacggca cgatcccgtc gcgccgcggc tcgatcaatg tggatgacga aggcaccccc
     4801 actcatcgca ctgtgctgat cgaaaaaggc gtccttaaag gctatctgca ggacaggctc
     4861 aatgcgggtt tgatgaagat ggcattgacc ggcaacggcc ggcgggaaag ttatgcgcat
     4921 attccgatgc cccgcatgac gaatactttt atgctggccg gcgattcggg tcccgacgag
     4981 attatccgct cggttaacaa aggtttgtat gcggtctcgt tcggcggcgg acaggtcgat
     5041 attaccagcg gtaaatttgt cttctcggcg agcgaagctt acatgatcga acacgggaag
     5101 gtcacccaac ctgttaaagg ggcaactctt atcggcaacg gtccggacgt tttaacccgc
     5161 gtgtccatgg tgggctccga tcttaaactc gacgagggag tcgggacctg cggtaaagac
     5221 ggtcaatcgg taccggttgg cgtcggtctg ccgacgatca aaatcaatgg actcaccgtg
     5281 ggcggaacaa gagcgtaaaa gaatttaatt ccaaccgttt aaatagttca aacagttcaa
     5341 gccgagtgtc ggcccaaatc ggatacgttt tgaacatttt gaacggcttg aatggattga
     5401 acccggaaga agtatggaac gtttcgaact gagccaagat ttggggtccg atattctgag
     5461 caaggcgaaa gcgaagggcg ccactcaggg tgatgtggtg atgtcggaga gcgagtcttt
     5521 ctttgtcacg gtgcggatgg gcgaagtcga gaagatcagc caggcgggtg aaaagcggtt
     5581 ggggttgcgg ctttttttcg gcaatagctc cgcttcggca tcgacgtccg acatttcgca
     5641 ccagtctatt gagaaactgg tcgatgacac ggcgcgaatg gccgagatca ccgca
//
LOCUS       DASULZ010000766         2384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_900658, whole genome shotgun sequence.
ACCESSION   DASULZ010000766 DASULZ010000000
VERSION     DASULZ010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900658"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VFY96_15770"
     CDS             <1..283
                     /locus_tag="VFY96_15770"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5607983.1"
                     /translation="GVVVGVISDRDISRALARNPNSRSKSLDQIMTKQPVTVKRNTAI
                     IDAVGKMLAKRINCLPVIDDEGRVCGIVTSTDLLKSYQQLLELVQKQAR"
     gene            338..565
                     /locus_tag="VFY96_15775"
     CDS             338..565
                     /locus_tag="VFY96_15775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEX5607984.1"
                     /translation="MARRIAVIVKPKARKPEIVKISPDVYRIAVREPAQNGQANKAAI
                     KFLSEHLGIPKSKLKIVRGVSSQHKLIEVMS"
     gene            819..1946
                     /locus_tag="VFY96_15780"
     CDS             819..1946
                     /locus_tag="VFY96_15780"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5607985.1"
                     /translation="MTEHLFNGVVVVVLPIITSSLGLSLAQAGALASARTLFAGVASF
                     PSGFFADLASRRNILLGTCIAMIGLASLGLSAAWTFPALMLFMALGGFGGGWFHPQSL
                     AILSTKYRDQKAFALGVHDSSANLGEIMGPLTIGFLLNFFDWRTSLQIWAIPGLCIGV
                     LYATFGREGNLAVPRPRDYRRAVWEDVLKNKAVFSLVAVSTLRAMGQTALAAFLPLYL
                     SLHLKLPASIAGAYMSILFLFAGMAPACVGWVSDRLGHKRLIAVFSVLSFVAIVTIPH
                     LGSGFLLTAGLAALGALLWALRPVIVSAAMSAAPQHLSGSIVALIYGANMGVSFLAPL
                     AAGLIADRYGLPIALTSISVFPLLAAIISCLVHPGAKTEDI"
     gene            complement(2027..>2384)
                     /locus_tag="VFY96_15785"
     CDS             complement(2027..>2384)
                     /locus_tag="VFY96_15785"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015861464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX5607986.1"
                     /translation="GTVAFVGDLGIYGNTVIIDHGLGLFTLYGHLSSIDVKVGDQVKQ
                     RQLIGKTGETGLAAGDHLHFGVYLHGLAVLPVEWWDQKWINDNVQPKLAGSTADTSEE
                     AKDKKPASRLTRKRRR"
BASE COUNT          476 a          647 c          661 g          600 t
ORIGIN      
        1 aggcgtggtg gttggggtca tttccgaccg cgacatttcg cgcgcattag cgcgcaatcc
       61 caactcgcgc tcaaagtccc tcgatcagat catgacaaaa cagccggtca cggttaagcg
      121 caatacagcg atcatcgatg ccgttggaaa aatgctcgcc aagcgcatca attgcctgcc
      181 ggtcattgat gacgagggaa gagtctgcgg aatcgttacc tccaccgatc tgctcaaatc
      241 gtaccagcaa ctcctcgaac tcgtgcagaa gcaagcgcgt tagtcatcgt tcttagatat
      301 cgttgatagc cggcgttctt tgcgtcatag atttatgatg gcgcgaagga tcgcggtgat
      361 cgtcaaaccg aaggcgagaa aacctgagat cgtcaaaatc tcgcctgatg tttaccgaat
      421 cgctgtgcga gaacctgcgc agaacggcca agcgaataag gccgccataa agttcctttc
      481 tgagcatttg ggcattccta aatcgaagct aaaaatcgtt cgcggcgttt catcgcagca
      541 taaacttatc gaggtcatga gttagagttg cggaaaacgt actgctccag ccttgagact
      601 ctcatcgacg gcaatttttt ctcgcctcta tcgaatctga ctttcaatcg agcggttccc
      661 ctcggttgtt tgatcgcagt tgcgcgcgat aaaaattcgg catatccatc gtagataatt
      721 ttgctcgagg gaagatcttg tcgaaggccg aaaacataca aacgctaaca aacccatttg
      781 ccggttgggg ccgactggcc gcgaactgca gcgcgcacat gaccgagcac ctcttcaacg
      841 gtgtcgtggt cgtggtattg ccgatcataa ccagctcctt gggattgagc ttggcgcaag
      901 ccggcgcgct cgcttcagcg cggacgcttt ttgccggcgt agctagtttc ccctcgggtt
      961 ttttcgctga tctcgcgagt cgaagaaata tcttgctcgg cacctgcatc gccatgatcg
     1021 gtttggcgag cctggggctg agtgctgcct ggacttttcc agctctgatg ctgtttatgg
     1081 cacttggtgg cttcggcggc ggctggtttc atccccaatc cctggcgatc ttgtcgacta
     1141 agtaccgcga ccagaaggcc ttcgcactgg gcgtccatga cagcagtgcc aatctcggcg
     1201 aaatcatggg tccgctgaca atcggtttct tgctcaactt cttcgattgg cggacaagcc
     1261 ttcagatctg ggcaattcca gggctatgta tcggagttct atatgcgacc ttcgggcgcg
     1321 agggaaacct cgccgtgccg cggccgcgag actaccgccg ggcggtgtgg gaggacgtgc
     1381 tcaaaaacaa agctgttttc agcctggtgg cggtttcgac gcttcgtgct atgggacaaa
     1441 cggcgctggc ggccttcttg ccgctttatt tgtcgttgca tctcaagctt ccggcgagca
     1501 tcgccggggc ctatatgtcg atcttatttt tattcgctgg tatggcaccg gcgtgtgtcg
     1561 ggtgggtgtc cgatcgccta gggcacaaac gattaatcgc ggtattttcc gttttgagtt
     1621 tcgtggcgat cgtgactatt ccgcacctgg gctcgggctt cttgcttact gctggtctgg
     1681 cggcgctcgg cgctttgctg tgggcgcttc gacccgtcat cgtatcggca gctatgtcgg
     1741 ccgcgccgca acacctatcc ggaagcattg tggccttgat ctacggcgca aatatggggg
     1801 tttcattcct ggctccactt gcggccgggc tgattgcaga tcgatacggc cttccgatcg
     1861 ctctcacaag tatttcggtt tttccgctcc tggcggccat catctcttgc ctcgtccacc
     1921 ccggcgccaa aactgaggat atttgacgcc catggaggcg aacacagcgc gctgatctct
     1981 gcggaaaaat agaaattatc gaggggaaat tatcgagtaa ggaaacttat cttcggcgct
     2041 ttcgggtcag tctcgaggcg ggttttttgt ctttcgcctc ttcgctggtg tctgccgtgg
     2101 agcccgcgag tttgggctgt acgttgtcgt tgatccattt ttgatcccac cattcgaccg
     2161 gtaaaactgc caagccatgc aggtagacgc caaaatggag gtggtcgccc gcagcgagcc
     2221 cagtttcacc ggttttgccg atgagttgac gctgcttgac ttgatcgccg actttgacgt
     2281 cgatcgaact caggtggcca tagagcgtga atagccccaa gccatgatcg atgatcacgg
     2341 tgtttccata gatgccgaga tcgcccacaa aggcgactgt gccg
//
LOCUS       DASULZ010000767         2349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1508916, whole genome shotgun sequence.
ACCESSION   DASULZ010000767 DASULZ010000000
VERSION     DASULZ010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508916"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..1163)
                     /locus_tag="VFY96_15790"
     CDS             complement(9..1163)
                     /locus_tag="VFY96_15790"
                     /inference="COORDINATES: protein motif:HMM:NF014346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030077 - plasma membrane
                     light-harvesting complex [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="photosynthetic reaction center cytochrome c
                     subunit family protein"
                     /protein_id="HEX5607987.1"
                     /translation="MSLGKRVRILITLAVFLCAMSLTLVSRAKEAAFTPQGTQAERTF
                     KNIQVLKGLPEGELYQTMNFIAVSLGENCTFCHVTNGRDPKTNQINWVWESDDKGEKQ
                     TARRMLQMVLLINGSNKVDFGPNAVTCHTCHRGQRTPEGLPSMPLAKSGHEGLLYPAP
                     LGPAAPRPRPSVDEIFAHYVQAIGPKAAATKTLIMKGTRLASQNRNWPNEIIWSAPDK
                     FLSVMTLPQTTIRQAVAGDAGWVLNGTKLQTLAAAQTTTARRGLEEVFSAVKVTQAPG
                     MQSAGIERIDGRDMWVVERSTPETTLKYYFDAETGLLRRRIAINHHGVLPLPEQTDFE
                     DYRDVDGVKLPFIIRRSAIDTYDSWTRTFAEIKRNVAVADALFAPPAVQP"
     gene            complement(1355..>2349)
                     /locus_tag="VFY96_15795"
     CDS             complement(1355..>2349)
                     /locus_tag="VFY96_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX5607988.1"
                     /translation="SFCGDDPTDAEGVGKIYDPAFNGMFSEEAMIALDAGRACDGGRP
                     PAELHFRDVRIPNPRKETPPRTILGADQKAWFKDQLRKSTATWKIWGNSLGALDWRSD
                     PQNLPAGMVVKPWPADTYAQFGSNDYGSAYAERKEIYDLVREAKITGFAIVSGDRHSF
                     WAGYAAAELPPRKFEPVGLSFVGASLASPGTMEAVEHRKNLPMRALFLADRPDGAKPD
                     WTYNMLVKHGVRSCLEYAKSFDLKRARSLSNPDLAPHLEFVDLGGHGYVKVQLSAAEM
                     RTEFVCIPRPIARSERPDGGPIRYRVLHTAALWKSGARPQLKTSVLEGDVGLAI"
BASE COUNT          464 a          679 c          656 g          550 t
ORIGIN      
        1 gaaagaagct agggctggac agccggcggc gcgaataagg catcagccac cgccacgttt
       61 cgtttgatct ccgcaaacgt acgcgtccaa ctgtcgtatg tatcgatcgc cgacctgcgg
      121 atgatgaatg gtaatttcac gccgtccaca tcacgataat cttcaaaatc agtctgttcg
      181 gggagcggca ggacgccgtg gtgattaata gcaatccttc ttcgcaagag gccggtctct
      241 gcgtcgaagt aatacttcaa ggttgtttcc ggagtcgacc tctccacgac ccacatgtcg
      301 cgcccatcaa ttctctcgat accggccgac tgcattcctg gcgcttgcgt aaccttgacg
      361 gcactgaaca cttcttccaa acctcgccgc gcggttgttg tttgtgctgc tgcgagcgtt
      421 tggagctttg ttccattcaa gacccagccc gcatcgccag ccacagcttg ccgaatagtt
      481 gtttgtggca gagtcatcac gctaagaaat ttgtcaggag cagaccagat gatttcgttt
      541 ggccagttcc ggttctgcga agccaagcgc gttcctttca tgatgagtgt tttggtcgct
      601 gccgctttcg gaccgattgc ctgaacgtaa tgcgcgaata tctcatctac tgacggtcgc
      661 gggcgcggcg ccgccggtcc caggggggcc ggataaagca agccttcatg tcccgatttg
      721 gccagcggca tcgacggcaa accctcgggc gttctctgac cgcggtgaca ggtgtggcag
      781 gttaccgcgt tcggcccgaa atcgacttta tttgatccgt taatcaatag gaccatctgc
      841 aacatgcgac gggcggtttg cttctcaccc ttatcatcgc tctcccaaac ccagttgatt
      901 tggttggttt taggatccct gccgttcgtg acgtggcaaa acgtgcaatt ctcgccaagc
      961 gacacggcta taaaattcat cgtttgatag agttctcctt ctggcaatcc tttgagcacc
     1021 tgaatatttt taaacgttcg ctcggcttgt gtaccctgtg gtgtgaaagc agcttccttc
     1081 gcgcgactca ccaatgttag tgacattgcg cataagaaaa cggcaagtgt gatgagaatg
     1141 cgaactctct ttccaagtga cattggtgat cctcctctga cctgcagtcg taatcgaaat
     1201 tcgaggcagt cggttgatct attctgctgt gtaagttgtc gacgaaagat attgttctgc
     1261 caagcacgct gcgatcactt agaaaaacga gcttaactgt ggctccagga gccttgtaaa
     1321 aatcagaaac ttcacacttt gagttgatgg tccgtcaaat cgcgagtccc acgtcgcctt
     1381 cgagcaccga cgtcttcagc tgcgggcgcg ctccactctt ccacagtgca gcagtgtgta
     1441 atacccgata cctgatgggc ccgccatccg gtctttcgct gcgcgcaatg gggcgcggga
     1501 tgcagacaaa ctcggtgcgc atctcagcgg cggacagttg gaccttcaca taaccgtgtc
     1561 ctccaagatc tacgaactcg aggtgcggag ccagatccgg gttggacagc gaccgtgcgc
     1621 gcttgaggtc gaaactcttc gcatattcaa ggcacgagcg gacgccgtgc ttcaccagca
     1681 tgttgtacgt ccagtcaggc ttcgcgccgt ccgggcgatc ggcgaggaac aaggcgcgca
     1741 taggcagatt cttgcgatgc tctacggctt ccatcgtgcc ggggcttgcc agcgacgcgc
     1801 cgacgaagct caatcccacg ggctcgaatt tccgcggcgg gagttcggct gcggcatacc
     1861 cggcccagaa gctatgccgg tcccccgaaa cgatcgcgaa gccggtgatc ttcgcttcgc
     1921 gcacgaggtc gtagatttcc ttgcgctcgg cgtaggccga tccgtagtcg ttactgccga
     1981 actgcgcata cgtgtccgcg ggccacggct tcacgaccat tccggcgggg aggttttgcg
     2041 ggtccgagcg ccagtcgagc gcgccgagcg aattgcccca gattttccat gtcgccgtcg
     2101 atttgcgtag ctggtccttg aaccaggctt tctggtcggc gccgaggatg gtccgcggtg
     2161 gcgtctcctt gcgcggattc ggaatgcgca catcgcggaa gtggagttcg gcgggcggcc
     2221 tcccgccgtc gcaggcgcgt cccgcgtcga gtgcgatcat cgcctcctca gaaaacattc
     2281 cgttgaacgc agggtcataa atttttccaa cgccctcagc gtcagttggg tcgtcgccgc
     2341 agaagctgt
//
LOCUS       DASULZ010000768         6223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_596533, whole genome shotgun sequence.
ACCESSION   DASULZ010000768 DASULZ010000000
VERSION     DASULZ010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6223
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596533"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..455)
                     /locus_tag="VFY96_15800"
     CDS             complement(39..455)
                     /locus_tag="VFY96_15800"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HEX5607989.1"
                     /translation="MAEARTPQKSLPKINKVDQRFWDGAAAGKLLLQKCKSCGKVQFF
                     PRVACVDCFGELDWIESKGSGKVHSFTLVRVPRNPAFKEETPIYYINVVLDEGVIMES
                     RLVGDSRDRVKMGDRVKAVFQETHDPKIKLPCFELA"
     gene            complement(455..1606)
                     /locus_tag="VFY96_15805"
     CDS             complement(455..1606)
                     /locus_tag="VFY96_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX5607990.1"
                     /translation="MRRSKVCFVGYGSTEYSRNSEASALSYYADAIRDALKRTGIDKK
                     EIRGFSITTQASPDYSPHVAEQLGLEVDWVLNGDYGGAGGVMSVRRAADAIECGHLDL
                     AVLVGGNSFDRSTPTQRQLEYQRGNFVDVFGYGGPNSLMALIQRLHMTNYGTTLEQIG
                     KIAVAQRFNALNNSQALFRQPMTMQDYLNSRMISDPIRLFDCVMPCSGAECILLASEQ
                     KAKQITDKLVYLVTDAEISHYQVANMLPDKTTFGQKVVGERIFSETKREDIDLFEIYD
                     DYPIAVMIQIEDLGYCKKGEGGKFVDNHDLTYNGDFPVNTGGGELSVGQAGLAGGYLH
                     LVEALRQLRGEAEGHQVKKAERALVTGIGWLNYGRNLGTTAALVMERRK"
     gene            complement(1700..2848)
                     /locus_tag="VFY96_15810"
     CDS             complement(1700..2848)
                     /locus_tag="VFY96_15810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5607991.1"
                     /translation="MLHPLRGNDMDLKLTEEEKLIKNAAAQFVDRELISREGDYLRQD
                     ARFLAPGAPSRRRLQPAVSEELAKRARQAGLWSLEIPEKLGGSPLSVVARVLVHREFG
                     RSILPFRPVSMPAFLFDTPYGERLAHGELSLALAFEEAHKTGELSASQILYRQEPDGT
                     TLRDSSIFVANSGADLFLFPAREQGSDRLGLFIIEREAPGVTITDEVDLTTDATVARL
                     ILRQCKVPNDRLLGYEYEAGEIIAAEQLNMAARSLGIGMRCLASAIEHARNRVTFGRR
                     LAERQAIQWMLADLSVEIRTCTWLTLEAASRADQELTYFDEAALAKKRATQMAFQAAD
                     TAIQIHGGYGVCKEFPFEGFYREARLMRLLYGRENELDRAAGEKFLKQ"
     gene            complement(2871..4067)
                     /locus_tag="VFY96_15815"
     CDS             complement(2871..4067)
                     /locus_tag="VFY96_15815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5607992.1"
                     /translation="MDFRLSAEEENLRKTVQDLVRDELIPLEAEFANAPDVFEGSRWK
                     SRVKSSADPEIKHYVAIMESLEKKAQAKGLWHLDVPKAYGGMEVSNIALIAVTEELEK
                     SSIPFELGNHVSNILYGCGGEQIDRYLWPCIRGEKTSAFGLSEPASGADPSMMQTTAT
                     PDGDFFVINGTKMFPTFADVADFVQLFARLPGTSGRDGVTCFLIDTGTPGYYVTRSIA
                     TIAGSEPCELVFENCRVHKSQVLGELGKGWALNQAWLGARRFQVGIRAHGKSARVLRI
                     VRDLVRHDDTARDRWASTIGHFLGENEALRTLTYLGAWKADQKLDVRAEAANVKLFAT
                     ELLHRVIDFALEVAGPSGLRKNHFLARAFRHARATRIVEGPSEIQRHIIQRTLFREGV
                     AGLELL"
     gene            4363..4863
                     /locus_tag="VFY96_15820"
     CDS             4363..4863
                     /locus_tag="VFY96_15820"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX5607993.1"
                     /translation="MKIRPAAIFSLCAFIFFCVFVYQAQEWRMQARLYPWAIGIPMLI
                     LAAIQVILDLKGVKAKQPTDGAPPTPMDFQFTQEVEPAIAKKRAITMFAWLFGFFVAV
                     LLVGFSIAIPLMMFTYMKFQGKESWVLSLVLTGIAFIFYYALFVRVLNLPFPEGLLLP
                     WLGLNG"
     gene            4991..6058
                     /locus_tag="VFY96_15825"
     CDS             4991..6058
                     /locus_tag="VFY96_15825"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5607994.1"
                     /translation="MNARKLFRFACCLAILAFGSTAIPTSGRGAEPKNEQFYSGKTVR
                     VIVGFTPGGFYDRWARLLSRYMPNYIPGTPNFVVQNMPGASSVIAANYVYNVAKPDGL
                     TLLVPINSLYLDQIVGRTEVKFDVRKFEFIGTQEKAPTMLYFRADTPFKSLADIIKAS
                     EPPKCGSTGTASTGYLIAKLLDEAFKAKLNTITGYQGGAEIDVAVERGEIICRGMDIP
                     PHFGREPFDSWHKKGFDRHILQGGSKRDPRLPETPTLFELMDQYKSSDVARRLTRIVL
                     ASGDFGRPMLAGPGTPPDRLKTLRDAYAKAMRDPGLIDEAKKGQMDMEYTPGEELQSL
                     MKEIMDQPAEVMDRVKKLLSD"
BASE COUNT         1303 a         1860 c         1649 g         1411 t
ORIGIN      
        1 tcctccggtc tttaatctaa aatccaaaat cgaaatagtc aggctaattc aaagcacggc
       61 aatttgatct tcgggtcgtg cgtctcttgg aacaccgctt tcacccgatc acccattttc
      121 actctgtctc tactgtcgcc caccagccga ctttccatga tcacgccttc gtccaaaacc
      181 acgttgatat aataaatcgg cgtctcttct ttgaaagccg ggtttcgtgg cacgcgcacc
      241 aaggtaaagc tatgtacttt gccgctgcct ttggactcga tccaatcaag ctcgccgaaa
      301 caatccacgc acgcgacacg cggaaaaaat tgcaccttgc cgcaactctt acatttctgc
      361 agcaaaagct tacccgcggc ggcgccatcc cagaagcgct gatccacttt gttaatcttc
      421 ggaagacttt tttgcggtgt tcttgcttca gccattattt tctcctttcc atcaccagtg
      481 ccgccgtggt tccgagattg cggccgtaat tgagccagcc gatacccgta accagcgcgc
      541 gctcggcctt ttttacctga tgcccctcgg cctcgcctct caactgacgc aatgcttcga
      601 ccagatggag atagccgccc gccaaaccgg cttgcccgac ggacaattcg ccgccgccgg
      661 tatttacggg aaagtcaccg ttgtaggtca aatcatgatt gtcgacgaat ttgccgcctt
      721 cgcctttttt gcagtaccca aggtcttcta tttggatcat caccgcgatc ggataatcgt
      781 cgtagatctc gaacaagtcg atgtcttctc gtttagtttc ggaaaaaatc cgctcgccga
      841 cgactttctg tccaaacgtc gtcttgtcgg gcagcatatt ggcgacctgg taatgcgaaa
      901 tctccgcgtc ggtgacgaga taaaccagct tgtccgtgat ctgcttcgcc ttctgctccg
      961 acgccaaaag gatgcactcc gcgcccgagc aaggcatcac gcaatcgaat aaccgaatcg
     1021 ggtcggaaat catccgcgaa ttgagatagt cctgcatcgt catgggctgg cgaaacaatg
     1081 cctgcgaatt gttgagcgca ttgaaacgtt gtgccaccgc gattttgccg atctgctcca
     1141 aggtcgtgcc gtagttagtc atgtgcaatc tctggatcag cgccatcaag gaattcggtc
     1201 cgccgtagcc gaacacatcg acgaagttgc cgcgctgata ctcgagctgg cgctgtgtgg
     1261 gcgtgcttcg gtcgaaggaa ttgccgccga ccagcaccgc cagatcgaga tggccgcact
     1321 cgatcgcatc cgctgcgcgc cggacgctca tcacgccgcc cgcaccgccg tagtcgccgt
     1381 tcaacaccca gtcgacttcc aaccctaatt gttccgccac atgcggcgaa taatcaggac
     1441 tcgcctgggt cgtgatcgag aatccacgaa tctctttctt atcgatgccg gtgcgcttga
     1501 gcgcgtctcg gatcgcatcg gcataatacg acagcgccga tgcttcggaa ttacgggaat
     1561 actccgtcga accatagcct acgaaacaaa ccttacttct tctcattctt tactcctcaa
     1621 atagggcgca gccgctgcga ccctatattt tcccgtccat cctgagtagg cgacacagtc
     1681 gccgcatcga aggactaccc tattgcttca agaatttctc cccggcagct cggtccagtt
     1741 cgttttcccg tccatataac aaccgcatca ggcgcgcttc gcgataaaag ccttcgaacg
     1801 ggaattcttt gcagacacca tagccgccgt ggatctgaat tgcggtatcg gccgcctgaa
     1861 atgccatttg ggtcgcgcgt tttttcgcca gcgcggcctc gtcgaagtaa gtgagctcct
     1921 gatccgcgcg cgaggccgct tcgagcgtaa gccaggtgca ggtccgtatt tcgaccgaca
     1981 aatcggcaag catccattgg atcgcttggc gctccgccag gcggcgcccg aaggtcactc
     2041 gattgcgcgc gtgctcgata gcgctggcca aacagcgcat gccgatgccg agcgagcgcg
     2101 ccgccatgtt caattgttcg gcagcgatga tctcgccggc ttcgtattcg taaccgagca
     2161 gccggtcgtt gggaactttg cattgccgga gaatcagcct ggcgacggtc gcatccgtgg
     2221 tgagatccac ttcatctgtg atcgtgacac cgggcgcctc tcgctcgatg ataaacaggc
     2281 ccaagcgatc gctgccctgt tctcgcgccg gaaacaaaaa caaatcggcg ccggagttcg
     2341 caacaaaaat cgatgaatcg cgcagagtcg tcccgtccgg ctcctggcgg taaagaattt
     2401 gcgacgcgct cagctcgccg gtcttgtgag cttcttcgaa agctaaggcc agagagagct
     2461 cgccatgggc gagtctttcg ccatagggcg tatcgaacag aaacgccggc atggagaccg
     2521 ggcgaaaagg taaaattgaa cggccaaact cgcgatgaac cagtacccgg gcaacgacgc
     2581 tcagcgggct tccaccgagt ttctccggta tttcgaggct ccagagtccc gcctgccgtg
     2641 cgcgcttggc caattcctcg ctgaccgcgg gctgaagtcg tcgccgcgac ggcgccccgg
     2701 gcgccagaaa gcgcgcgtct tgtctcaagt aatctccctc gcgggaaatt aattccctat
     2761 cgacaaattg cgccgctgcg tttttgatca gcttttcttc ttcggtgagc ttgagatcca
     2821 tatcgtttcc gcgtaggggg tgcagcatgc tgcgccgcca tgctgcggcc ctatagcaat
     2881 tccagccctg cgacaccctc gcgaaagagc gtgcgttgaa tgatgtgtct ctgaatctct
     2941 gacggacctt caacgattcg ggtcgcccgc gcatgacgaa acgctcgtgc aagaaagtga
     3001 tttttgcgga gccccgatgg cccggcgact tctagggcga agtcgatcac tcgatgcaaa
     3061 agctcggtgg cgaagagttt gacattcgcc gcttcggcgc gcacgtccag tttctggtcc
     3121 gctttccagg cgccgaggta cgtgagcgtc ctcagagctt cgttttcgcc gagaaagtga
     3181 ccgatggtcg aagcccatcg atcgcgggct gtgtcgtcgt gccgcaccag atcccgtacg
     3241 atgcgcaaca cgcgcgctga ttttccatgg gcgcgaatac ccacctggaa tcgccttgct
     3301 ccgagccacg cctggttcaa agcccagccc ttaccgagtt cgccaagaac ctgggacttg
     3361 tgcacgcggc agttttcaaa aaccagctcg caaggttcgc tgcccgcgat cgtcgcaatg
     3421 ctgcgcgtga cgtaatagcc gggtgtgccg gtgtcgatta aaaagcaagt gacaccgtct
     3481 cgaccgcttg tgcccggcag acgagcgaag agctggacga aatcagcgac gtcggcaaac
     3541 gttgggaaca ttttcgtgcc gttgatcacg aaaaaatcgc cgtcaggagt tgccgtcgtt
     3601 tgcatcattg acgggtcggc cccggaggcc ggctcgctca agccgaatgc cgaagttttt
     3661 tcgccgcgaa tgcacggcca gagatacctg tcgatctgct cgcctccgca gccgtagaga
     3721 atgttcgaca cgtggttgcc gagctcgaac ggaatcgagc ttttctccag ttcctcagtg
     3781 accgcgatca gcgcgatgtt gctgacctcc atgcccccgt aagctttcgg cacgtcgaga
     3841 tgccacagac ccttggcttg cgctttcttt tccaggcttt ccatgatcgc gacgtaatgt
     3901 ttgatctccg gatctgcgct tgacttcacc ctacttttcc agcggctgcc ctcgaagaca
     3961 tccggcgcat tggcgaattc agcttccaga ggaatgagct cgtcccggac taagtcctgg
     4021 acggttttgc gcaggttttc ttcttctgcg gagaggcgaa aatccatcgg cttggtgacc
     4081 tcggcgcgac atttatcgat ggcgcggcgt ggtttctgac gattagctca cttaccacac
     4141 cccgcggcgg caacggaaga gcaaaggcat tgacccatca atcgcctcta tgctaggcgg
     4201 aaacgagagt cggtttagca gcgcgccagc cgagtaccgt cattcgagcc cgcttcagga
     4261 tcgcgataga cgaacgatga aaactttgct agattccgct gctccgatga tgttccgcag
     4321 tggcggccag ttaaaaatca gaaagcgtag ggggattatc cgttgaagat cagaccggcg
     4381 gccattttca gtctttgcgc gttcatattt ttctgcgtgt tcgtttatca agcccaggag
     4441 tggcgcatgc aagcgcggct ttatccctgg gctatcggca ttccgatgct gattctcgcg
     4501 gcgatccagg tgatcctcga cttaaagggc gtcaaggcca agcagccgac cgacggagct
     4561 ccgcctactc cgatggattt tcaattcact caggaagtcg agccagcgat cgccaaaaaa
     4621 agagcgatca ccatgtttgc ctggctgttt ggttttttcg tcgcggtcct gctcgtcgga
     4681 ttttcgattg ccataccctt gatgatgttc acctacatga agtttcaagg gaaagaatcg
     4741 tgggtcttat cgctcgtttt gaccgggatc gcgttcattt tctactatgc actttttgtc
     4801 agggtgctca atctgccctt tcccgagggc ttgctcctcc cgtggcttgg attgaacggg
     4861 tagttcgcgt tctttgcagc gaccgccgga ttggattttc acgcgatgtc ttgatcgctg
     4921 attcgcgcac ctactcataa tcgatttcac tgacagcatc atgtcggttg ttttcattga
     4981 gggctttacg atgaacgcgc gaaaactctt ccgcttcgcc tgctgcctcg cgattcttgc
     5041 gtttggctca accgcaatcc cgactagcgg ccgcggcgcg gaaccaaaga acgagcaatt
     5101 ctacagtggc aaaacagtgc gtgtcatcgt aggcttcaca cccgggggat tctatgaccg
     5161 ctgggcaagg cttctgtcgc gctacatgcc caattacatt cccggcactc ccaacttcgt
     5221 cgtgcaaaat atgcctggcg cgagctcggt gatcgcggcc aactacgttt acaacgtcgc
     5281 gaagcccgac ggcctgactt tgctcgtacc gatcaacagc ttgtacctcg accagatcgt
     5341 cggccgcacg gaagtaaaat ttgatgtgcg gaaattcgaa ttcatcggca cccaggaaaa
     5401 agcgccaacc atgctctact ttcgcgccga tacgccgttt aaatctctcg ccgatattat
     5461 caaggcgagc gagccgccca aatgcggctc caccggcacc gccagcaccg gttacctgat
     5521 cgccaagctc ctcgacgagg cgttcaaggc aaagctcaac accattaccg gctatcaagg
     5581 cggcgcggag atcgacgtcg cggtcgagcg aggcgaaatc atctgccgcg gcatggacat
     5641 cccgccccat ttcggccgcg agccgttcga cagctggcac aaaaagggat tcgatcggca
     5701 tattctccag ggcggctcca agcgcgatcc gcgcttgccg gagacgccga cgctcttcga
     5761 attgatggat caatacaaat cttccgacgt ggcacggcgt ttaacccgca tcgtgctcgc
     5821 cagcggcgat ttcggccggc cgatgctcgc cggccccggc acgccgcccg accgtcttaa
     5881 aacgctgcgt gacgcctatg ccaaagcgat gcgcgatcca ggcttgatcg acgaggcgaa
     5941 aaaaggacaa atggacatgg aatatacgcc gggcgaggag ctccagagct tgatgaaaga
     6001 aatcatggat cagcccgccg aagtgatgga ccgggtaaaa aagcttctgt cggactgacc
     6061 ggcgcccctc ctcgcctgct ccctaaagat tttgccggcc gtcgaatcga tgagcgcccg
     6121 cgctcgtcta aagattggac agggtcaacc gacgggaacc agtcggttga ttctacagtt
     6181 ttaaatggta cggttccggc atatttcttt caggaggttt caa
//
LOCUS       DASULZ010000769         3123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_494591, whole genome shotgun sequence.
ACCESSION   DASULZ010000769 DASULZ010000000
VERSION     DASULZ010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494591"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(251..409)
                     /locus_tag="VFY96_15830"
     CDS             complement(251..409)
                     /locus_tag="VFY96_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5607995.1"
                     /translation="MVVNTNLEEIVQRYTIRFGDIKPDWSVFGDSNYDGYRRAQFRYI
                     GARSVSPR"
     gene            complement(530..1570)
                     /locus_tag="VFY96_15835"
     CDS             complement(530..1570)
                     /locus_tag="VFY96_15835"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607996.1"
                     /translation="MAKSRKIICEKLLFAVLSGALVIALTVAGSAQERIRLSYSALSP
                     STAFLWIPKEKGFFKKHGLEVEILLIESGSLTSQALAAGEIGIADNAGAPAIISNASG
                     SGETIIMGLVNSLAYNMVATKQVKDLADLKNRRIGVSRIGSSSHAAVAIALDHFKIDA
                     TRDHITYVQSGTMTTRIAGMRAGSIDATVVDPGFVPFLVSEGFKDLGYLGKFGIPYQH
                     ESLDSSKAFLAKHRATALNAVKGIIEGIAFIAQERNAPEVKRVLAKYLKFEEQAKIDD
                     AYTSLRGYAVSIRKPYPTDEGVDSLIKFLAKFNPKVANVTVKDVVDASLVAELDKSGF
                     IDSVYKEMARGK"
     gene            complement(1949..3001)
                     /locus_tag="VFY96_15840"
     CDS             complement(1949..3001)
                     /locus_tag="VFY96_15840"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5607997.1"
                     /translation="MKQKKNLSSWMGSAIFVWTIAVALIICGASAGKLFAAAADSLDQ
                     LYEKSKKEGKITLYAPLSPSAMSVVPPAFMKRFPGITVDHIDATSDKLIARVLSEQKG
                     GRVLADVISGALTYLPQILDLKIVEHAVIPEAAAYPAQLKGDLWIATDTQYYIAGWNT
                     NLVKKGEEPKSYEDLANPKWKDGIMGEPRDYQMLVGFAKRKYNNDEKAIDLFKKIAAN
                     QVEFHRGHSQLTEFLMAGQRAICFTCYSHHFPMRMKKGAPIQPLMVEGVGEVGGSVMI
                     MKGAPHPNAALLWVRWAVSEEGQKVYAQAGETPAHPKVEPLEKIRPATPYMLTIDDVR
                     EFPKYEKMWKEIFQIR"
BASE COUNT          667 a          854 c          827 g          775 t
ORIGIN      
        1 atggatttta tagagcggat cttcggcatc tcgccggatg gcggcgacgg ttcaacggag
       61 ctgatcattg tcagtgtgtt ggtcttgctc gccctcatgg ccgcgtggac gtggtggtcg
      121 aaacgatcgc aagacgctta gtctgcgaat aattggcggg aacaaccacc gggtgttgtg
      181 ccgcgtgtag ggcgcattcc cggcggcacg tacatcacag acaaccgcgt ggcaccggcc
      241 ggaatatgtc ttacctcggg cttaccgagc gtgcgccgat gtagcggaac tgcgcgcgac
      301 gatagccgtc gtagttcgaa tcaccgaaaa cgctccagtc cggtttgatg tcgccgaagc
      361 ggatcgtgta gcgctgcaca atctcttcga ggttcgtgtt aacgaccacg gggatgacgc
      421 ggatgccgag cctttactca gctcgtactc ctttaggcgt gttcgagggc ctatggctcg
      481 aatcacgcat gctcactgca caagaggtga aaacctaaaa attcagcgct cattttccgc
      541 gagccatttc cttataaacc gaatcgataa agccgctctt gtcgagctcg gccaccaaac
      601 tcgcatcgac gacatctttc accgtcacat tcgcaacctt aggattgaac ttcgccagaa
      661 atttaatcag gctgtcgacg ccttcatcgg tcgggtacgg tttgcgaata ctgacggcgt
      721 aaccccgaag cgacgtataa gcatcgtcga tcttcgcctg ctcctcgaat tttaaatatt
      781 ttgccaggac ccgcttcacc tccggggcat ttcgctcttg ggcgataaac gcaatgccct
      841 cgataatccc cttgaccgcg ttcaaagccg tggcccggtg tttggccaga aaggctttgc
      901 tgctgtcgag cgactcgtgc tgatagggaa tgccgaactt gccgagataa ccaagatcct
      961 tgaatccttc gctcaccaga aacggcacaa atcccggatc gaccaccgtt gcatcgatcg
     1021 aaccggctcg catgccggcg atgcgcgtcg tcatcgtgcc gctctgcaca taggtgatgt
     1081 ggtcgcgtgt ggcgtcaatt ttgaagtgat cgagggcgat ggcgacggcg gcatgagagg
     1141 agctgccgat ccggctcact ccgatgcgcc ggttcttgag gtcagccaga tctttgacct
     1201 gcttggtcgc aaccatgttg taagccagcg aattgactaa tcccataatg atcgtttcgc
     1261 cggaacccga ggcattcgag atgatcgccg gagcgcccgc gttatcggcg atgccgattt
     1321 cgcctgccgc cagcgcctgt gaggtaaggc tgccgctctc gatgagcagt atttcgactt
     1381 ccaggccgtg ttttttaaag aatccttttt ctttcggaat ccagagaaac gcggtgctgg
     1441 gactgagcgc cgagtagctc agccgaattc tctcctgagc cgagcccgca accgttaagg
     1501 caataacgag ggcgccggac agaacagcaa aaagaagttt ctcgcaaatg atcttgcgac
     1561 ttttggccat gctcattgct ctccgtttcc ttggtcccga ctataaatgt tcaatacctg
     1621 ccttctcgca ataagcccag acaaaagttt ggccgaaaga tgcgcttgtt tggcgaggca
     1681 gaaggtagtt tcgtactttt gctgtccact atcgattacg attcgtgaag caggatcaaa
     1741 tctttatttt gatcgtcctg caaggggaga tagctggctc tcagacttat tttctatttc
     1801 ttcgcgtggc ccagggcaca gcttgccgca cgcgtttgat ggggtgtgag ccgcgatggc
     1861 atcgcgatcc gggaaagatc tagactgaag gaagatccaa gggttttcag gacctgcgtg
     1921 tggtcaggtg gcacaccgct cctgaatttc aacggatctg aaagatttcc ttccacattt
     1981 tctcgtactt gggaaactcc cggacgtcgt cgatggttag catgtaaggc gtcgcaggcc
     2041 ggattttttc cagaggttcc actttcggat gagcaggagt ttcacccgct tgagcataaa
     2101 ccttttgtcc ttcttcgcta actgcccagc gaacccacag gagcgcagcg ttgggatggg
     2161 gcgctccctt catgatcatc acagagccac cgacttcgcc cacaccttcc accatcaacg
     2221 gttgaatcgg cgcgcctttt ttcatgcgca tcgggaaatg gtgcgagtag caagtaaaac
     2281 aaatggcgcg ctgccccgcc ataaggaatt cggttaactg cgaatgcccc cggtgaaact
     2341 ccacttgatt ggcggcaatt ttcttgaata gatcgatcgc cttttcatcg ttgttgtatt
     2401 tacgtttggc aaatccgacc agcatttgat aatcgcgagg ctcgcccatg atgccgtctt
     2461 tccatttcgg gttcgcgaga tcctcgtagc tttttggctc ctcgcccttc ttaaccagat
     2521 tcgtgttcca tccggcgata tagtattgcg tgtcggtggc gatccaaagg tcgcctttta
     2581 gctgggccgg ataggcagct gcctcgggta tgacagcgtg ctcgacaatt ttcagatcta
     2641 ggatttgcgg caagtaggtc aacgctccgc tgataacatc ggcgaggacg cgtccgccct
     2701 tctgctcgga gagcactcgc gcgatcagct tatcgctcgt ggcgtcgata tgatcgaccg
     2761 ttatgccggg aaagcgcttc atgaacgcag gcggaaccac cgacatcgcg ctaggggaca
     2821 gcggcgcata aagcgtaatt tttccttcct tctttgattt ctcataaagt tgatcaaggg
     2881 agtccgccgc ggccgcgaaa agtttaccgg cagaagcgcc gcagatgatg agcgccacgg
     2941 cgatggtcca tacaaagatt gcggatccca tccagctcga taaatttttc ttttgcttca
     3001 tttcacttct ccctagtttt gtggttccac cattctgaat tcatctccgg gaaaacttcg
     3061 tccctgcgcc tacgttaagg gtttctgcca ctcctcaatg taggaaaaca gcgataatgt
     3121 caa
//
LOCUS       DASULZ010000770         3740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_697987, whole genome shotgun sequence.
ACCESSION   DASULZ010000770 DASULZ010000000
VERSION     DASULZ010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697987"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /gene="dnaJ"
                     /locus_tag="VFY96_15845"
     CDS             <1..986
                     /gene="dnaJ"
                     /locus_tag="VFY96_15845"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX5607998.1"
                     /translation="GDKQAEEKFKEVSEAYSVLSDPHKRAQYDQFGHAAFGEGGPGFD
                     FSGGFEDIFGDIFGEFFGGGGRRRGRARGEDLRYNLTLKFEEAVSGTDKKIKIPRHGV
                     CDSCRGSGAKTGTAPQTCPTCRGRGQVSFQQGFFSVSRTCSQCHGQGTVIKEPCATCG
                     GAGRIRNMHTLSVRIPAGVDTGSRLKLRGEGEAGPAGGVPGDLYVVIHVEPHPIFVRE
                     NLDIICDVPISFVQAALGADIDVPTLEGKVKMKIPPGTQSGKVFRMKAKGVKDVQGFQ
                     QGDQHVRVTVEIPTRLTAKQKELLKEFAALGGEELNPLAKGFFDKMKELFG"
     gene            1110..3590
                     /gene="lon"
                     /locus_tag="VFY96_15850"
     CDS             1110..3590
                     /gene="lon"
                     /locus_tag="VFY96_15850"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX5607999.1"
                     /translation="MSEAEWKAENAENKTSQVEIPEILPLLPIRDIVIYPYMMLPLFV
                     GRDVSIRAVEEALSRDRLIFLVSQMNSSEENPAPADIHRVGTIASIMRMLKLADGRVK
                     ILVQGLAKGEVDTYLRERPFFEVKIKKVIEPTLAEVPIEVEALMRTAKEKIEQILNLK
                     NLPPEIVIVTDNISDPGVLADLVASNLRLKIEESQAILEIFDPIERLKKVNELLAREL
                     ELSTMQARIQNQAKEEMSKTQRDYYLREQLKQIQQELGEGDERAEELNELKKQIEKAK
                     MPLEVKREADKQLRRLEQMHPESSEASLVRTYLDWLVDLPWSKRTKDNLNIKKAKQVL
                     DEDHYDLEKIKERILEYLAVNKLRRKIKGPILCFVGPPGVGKTSLGRSIARSLGRNFV
                     RVSLGGVRDEAEIRGHRRTYVGALPGRVIQGIKQAGSNNPVFMLDEIDKVGADFRGDP
                     SAALLEVLDPEQNHAFSDHYLNVPFDLSNVLFICTANLLDPIPAALADRMEVIQLSGY
                     TNEEKLAIARKYLVPRQFTDNGISATNLEISDDAVLRVIAEYTKEAGLRNLEREIASI
                     CRKVARKVAEGKTELTRVNRANIHSFLGAPKFLPEAEQEHHEIGVATGLAWTSTGGEI
                     LYVEASLSRGRGNLTLTGQLGDIMKESAQAAVSYARAHAKELGIEADFYQKLDIHIHV
                     PAGAIPKDGPSAGITMATALISALTKRPVSRDVAMTGEITLRGRVLPIGGLKEKSLAA
                     FRAGIKTLVVPERNQKDLDEIPKVLRRKLNWVLAKNMSDVLSVALMERRKGSTKSSAT
                     ERILPRQPLVAKPLRKSARERSVPIRPN"
BASE COUNT          909 a          949 c         1089 g          793 t
ORIGIN      
        1 caggcgataa gcaagcagag gaaaaattca aggaagtatc ggaagcgtat tcggtgcttt
       61 ccgatcccca taagcgcgcg cagtacgatc agttcggtca tgcagcgttt ggtgagggag
      121 gacccgggtt cgatttttcc ggtggattcg aagacatttt cggtgacatt ttcggcgagt
      181 tttttggggg cgggggacgc cgacgcggtc gcgcacgcgg cgaggatcta cgttacaatc
      241 tgacccttaa gtttgaagag gctgtttcgg ggactgataa aaagatcaaa attccgcgtc
      301 acggcgtgtg cgacagttgc cggggcagcg gggcaaaaac cggaacagcc cctcaaacat
      361 gccccacctg ccgaggtcgg ggccaggtca gttttcagca agggtttttt agcgtttccc
      421 ggacgtgcag ccaatgtcac ggtcagggga ccgtcattaa ggagccctgc gctacctgcg
      481 gcggggcagg gcgcatccgt aacatgcaca ctctcagtgt gaggattcct gcgggagtgg
      541 acaccgggtc acggctaaag ctgcgcggcg aaggcgaagc cggccctgcg ggcggcgtgc
      601 ccggcgatct ctacgtggtc attcatgtgg agccgcatcc gattttcgtg cgggaaaatc
      661 tcgacatcat ctgtgacgtg ccgatcagct tcgtccaggc ggcgttgggt gccgacatcg
      721 atgtgcccac gctcgagggc aaagttaaaa tgaagatccc gcccggcacg caatccggca
      781 aagtttttcg catgaaggca aaaggggtaa aagacgttca gggttttcag cagggtgacc
      841 aacatgttcg cgttaccgtc gaaataccca ctcggctgac ggccaagcaa aaagagttat
      901 tgaaggaatt tgccgcactc ggcggcgaag aactcaatcc tctcgcgaag ggctttttcg
      961 ataagatgaa ggaactattt ggctgagccc ttcgtagatc tccggcgctc gaatctgttc
     1021 ccgccatcgc ggatgtttat cgctgcgcag cggtttgtgt tgaccttatc gcgccagtac
     1081 gattaaggtg ctctgagaat ggagccataa tgagcgaagc tgaatggaaa gctgaaaacg
     1141 cggagaacaa aacctcccaa gtcgaaattc cggagatttt accgctcctc cctatccgcg
     1201 acattgtgat ttatccctac atgatgctgc cgctctttgt cggtcgggat gtttcgattc
     1261 gtgcggtcga agaagcgttg agtcgcgatc ggttgatttt cttggtctcg cagatgaatt
     1321 cttcggagga gaatccggct ccggccgata ttcaccgcgt gggcacaatt gcgtcgatca
     1381 tgcgcatgct gaagctcgcg gacggccgcg tgaagattct ggtccagggt ttggcgaaag
     1441 gcgaagttga tacctatttg cgcgagcgac cgttctttga agtgaagatc aaaaaagtca
     1501 tcgagccgac tttggctgag gtgcccatcg aagttgaagc tttgatgcgc acagcgaaag
     1561 aaaaaatcga gcagatatta aacctcaaga acctgccgcc cgaaatcgtg atcgtaacag
     1621 acaacattag tgatcccggt gtactggccg accttgtggc ttccaacttg cggctcaaaa
     1681 tcgaagagag ccaggcgatt ctcgaaattt ttgatcctat cgagcgttta aaaaaggtca
     1741 acgagctgct tgcgcgagag ttggagctct cgacgatgca ggcgcgcatc cagaatcaag
     1801 ccaaggagga aatgagcaag acccagcggg attactacct gagggagcag ctcaagcaga
     1861 ttcaacagga actgggcgaa ggagacgaac gcgccgaaga gctcaacgag ttaaagaaac
     1921 agatcgaaaa agccaagatg ccactcgagg tcaagcgtga ggccgataag caactccgcc
     1981 gactcgaaca gatgcatcca gagtcgtctg aggcatcctt agtgcgaacc tacttggatt
     2041 ggctcgtgga tttgccatgg agcaagagga ccaaggacaa tctgaatatc aaaaaggcca
     2101 aacaggtcct cgacgaggat cattacgacc tggaaaaaat aaaagagcgc atcttggaat
     2161 atttggcagt gaacaagctc cggcgcaaaa taaaaggtcc gattctttgc tttgtcggtc
     2221 ctccgggagt tggcaaaact tctttaggac gatccattgc ccggtccctc gggaggaact
     2281 ttgtgcgcgt ttcgcttggc ggcgtccgtg atgaagcgga gattcgcggc catcgccgca
     2341 cctatgttgg cgcgctgccg ggacgtgtta ttcagggaat caaacaagcg ggttcgaaca
     2401 acccggtctt catgctcgat gaaatagata aagtcggagc tgatttccgt ggcgacccat
     2461 ctgccgctct gctcgaagta ctggatcccg aacagaatca cgctttcagt gatcactatt
     2521 tgaacgttcc gtttgatctc tccaatgtgc tgtttatctg cacggccaac ttgctcgatc
     2581 cgatcccggc agcattagcg gaccgtatgg aagtcataca actgtccggc tatacgaacg
     2641 aagaaaaact ggcgatcgcg cgtaagtatc tcgttcctcg acagttcacc gataacggta
     2701 tttctgcgac gaacctagag atctcggatg atgcagtgct gcgggtgatt gcagagtata
     2761 ccaaggaagc cgggcttaga aacctggaac gcgaaattgc ttcgatctgc cggaaagtgg
     2821 cgcgcaaagt agccgagggc aaaactgaac tcactcgagt gaatcgcgcc aatattcatt
     2881 cttttctggg tgcgccgaag ttcttgcccg aagctgagca ggagcaccac gaaatcggcg
     2941 tagctacggg actggcatgg acgagtaccg gcggggagat actatacgtc gaagcttcgc
     3001 tgtcgcgcgg ccggggtaat ctgaccctga cgggacaact tggggacatc atgaaggaat
     3061 cggctcaagc cgcggtgagt tatgctcgcg cccatgccaa agagttgggg atcgaagcgg
     3121 atttctatca gaagctggat attcacattc acgtcccggc gggggccatt ccgaaggatg
     3181 gcccttcggc cggtattacc atggctaccg cgcttatctc agctctgacc aaacggccgg
     3241 tcagcaggga tgtggcgatg accggtgaaa ttaccttgcg cgggcgagtc ctgccgatcg
     3301 gtgggcttaa ggaaaaatcg ctcgccgcct tccgggcggg catcaaaacg ctcgtcgttc
     3361 ccgagcgaaa tcaaaaggac ctcgacgaaa ttccgaaagt gttacgacgc aagctcaact
     3421 gggtgttggc caagaacatg tccgacgttt tatccgttgc tttgatggag cggcgtaagg
     3481 gcagcaccaa atcgagcgct acggagcgaa ttctgccgcg gcagccgctg gttgcaaaac
     3541 cgctgcgaaa aagcgcgcgg gaaagatctg ttccaattcg acctaactga catccgatta
     3601 ttctgttcct tccgcgataa tcgcctgcag aaattcagcg cagcgcgcga tgaaactcag
     3661 ccgcctcgga gaattcggtc tcatcgagag aattcggcag aagacaccgg tcgggcgggg
     3721 cgtccggatc ggcatcggcg
//
LOCUS       DASULZ010000771         2122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_495043, whole genome shotgun sequence.
ACCESSION   DASULZ010000771 DASULZ010000000
VERSION     DASULZ010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495043"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VFY96_15855"
     CDS             <1..764
                     /locus_tag="VFY96_15855"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5608000.1"
                     /translation="AGETQFHVGSATGTSIGAMAGGLDLAYIAGLINKLDGAFMVNPS
                     IKMPADLKGKRVGVQSIGGGVWMFSMLAFEHWGLNPERDNIQFRIIGDQSVLAQAITQ
                     NIIDGGYLGYAYGAQLERQGYRMLGDLMKMGIPYQGLGMIAKRSLIDRTPEIPERTLR
                     AMVEAIAYVNNPANKGAVVKTLTRELRLSKIEDAQAGYEMMRTLYDKKIYPNVDGLRN
                     VVRLLGKTSEPIRKLKVEDIIDDRIVRKLEKEGLF"
     gene            930..1991
                     /locus_tag="VFY96_15860"
     CDS             930..1991
                     /locus_tag="VFY96_15860"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5608001.1"
                     /translation="MAGNGSDAGFEVLISSDSHVMEPSEILAERAPQSYKDQVPRFPK
                     LEVGSSFQTHPGGSDPSHRIKEMETDGLSAEVLYPTYLLPHFAMDDAKLQEVSFRAYN
                     DWLIDYCRTSPKRLIGIAAVSVYNIDEAVKELERCAKAGMKGSIIWQAPHPDLPFHSE
                     HYNRYWAASEDLNMPVHLHILTGHGYHKESVFGNKRTGVEAYRGSVNLKLKEIIDDIF
                     ELIFYGVLERYPKLKIVSVENEIGWMPFMMQQWDYYYNRFKKVNPPPFTKNPSEYFKT
                     QIFGTFFRDTVAGHNFEWWGQDNCMWSNDYPHGNSTWPESKKYIDRNLGHLPQDIRKK
                     LVYSNVRNLYDIDIPHPVQ"
BASE COUNT          530 a          553 c          580 g          459 t
ORIGIN      
        1 ccgctggcga aacccagttt catgtcggct cggcaaccgg gacatcgatc ggcgccatgg
       61 cgggcggttt ggatctggct tacatcgccg gtttgatcaa caagctcgac ggcgcgttta
      121 tggtgaatcc gagcatcaaa atgccggctg atttgaaggg caagcgagtc ggtgtgcaaa
      181 gcatcggcgg cggagtttgg atgttttcca tgctcgcctt cgagcattgg ggcctcaatc
      241 ccgagcgcga caatattcaa tttcgcatca tcggcgatca atccgtgttg gcgcaggcga
      301 tcacgcaaaa catcatcgac ggcggctacc tcggctatgc ttatggcgca cagcttgaac
      361 gccaaggata ccgcatgctc ggcgatctga tgaaaatggg cattccctac cagggactcg
      421 ggatgattgc taagcgcagt ctcatcgatc gcacgccaga gattcccgag cgtactttgc
      481 gcgccatggt cgaagcaatt gcctatgtca ataacccggc taacaaaggc gctgtggtga
      541 aaaccctgac ccgagagctg cgcctgtcga agatcgagga cgcccaagcc ggctatgaga
      601 tgatgcgcac gctttacgac aagaaaattt acccgaacgt ggacggtttg cggaatgtgg
      661 tgcggttgct cggaaaaacc agcgagccca tccgcaagct caaggtcgaa gacatcatag
      721 atgatcggat cgtcagaaaa cttgagaagg aaggactgtt ctgaaaatcg gagtattgga
      781 gttatggagt gacggagtgt tgggttggaa cgcaatactc cagtatccaa ccactccagt
      841 tcccgtaact tgacccttca gcgctcttgg tggtagacct gcgaacagat cgcaaatagg
      901 acaattacag cccaagagga gaaaaattta tggctggcaa tggcagcgac gcaggattcg
      961 aagtactaat ttcctcagac tcccacgtta tggagccctc ggagattctc gccgaacggg
     1021 cgccccaatc gtacaaggac caagtgccgc gcttccccaa attggaggtg ggcagcagct
     1081 ttcagactca tccgggcgga tcggatcctt cgcaccgcat taaagagatg gaaacagacg
     1141 gtctgagcgc cgaggtccta tatccgactt acctcttgcc gcatttcgcg atggatgacg
     1201 ccaagcttca ggaagtgagc ttccgcgcgt acaacgattg gttgatcgat tactgtcgga
     1261 cctcgccaaa acggctgatc ggcattgcgg cggtttccgt ctacaatatc gatgaagccg
     1321 tgaaggaact ggagcgctgc gccaaggccg gcatgaaggg gtccatcatc tggcaggcgc
     1381 cgcatccgga tttgcctttt cactccgaac actacaatcg gtattgggcg gcatccgagg
     1441 atctgaacat gccggtgcac ctgcacattt tgaccggaca cggctatcac aaagaatctg
     1501 tcttcggcaa caaacgcacg ggtgtcgagg cctaccgcgg cagcgtcaat ttaaaattga
     1561 aggagattat cgacgatatt ttcgaattga ttttttatgg tgtgctcgag cgctacccga
     1621 agcttaaaat cgtcagcgtc gaaaatgaaa tcggctggat gccgttcatg atgcagcagt
     1681 gggattacta ttataatcgc ttcaaaaagg taaacccgcc gccatttacg aaaaatccca
     1741 gcgaatattt taaaacccag attttcggca cgttcttccg cgatactgtg gcgggccata
     1801 acttcgaatg gtgggggcag gacaactgca tgtggtccaa cgattacccg cacggcaact
     1861 cgacctggcc agagtcgaaa aaatacatcg atcgcaacct cggccactta ccgcaggata
     1921 ttcggaaaaa gcttgtctac agcaacgtaa gaaatctcta cgacatcgac attccgcacc
     1981 cggtgcagta ggggccgtga gcttgctagt gtgatttggc gcgctgacag gcgttaggca
     2041 gtctgacagc gcgccatttt tttatgtgtt caggaaacaa tcagccgcac gaataacgcg
     2101 caggaggttt tatgcccgcc cg
//
LOCUS       DASULZ010000772         2018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_901953, whole genome shotgun sequence.
ACCESSION   DASULZ010000772 DASULZ010000000
VERSION     DASULZ010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901953"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(370..1608)
                     /locus_tag="VFY96_15865"
     CDS             complement(370..1608)
                     /locus_tag="VFY96_15865"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020150603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX5608002.1"
                     /translation="MSTNLISSGTTARQKVNLRTPDVMAAVQQQVESHYRSDIIEKVR
                     RAGGTISVGDTTVRLAKQFGFCYGVERAIDLAYAARKVFKDRRLFIVGEIIHNPEVNH
                     QIASLGIKNLTGKNKEADIADLGPDDVVIVPAFGTELSIQQQIKDRGCQIVDTTCGDV
                     MSVWKRVRKYAIESATSIIHGKAEHEETKATSSRALGDGSGHYLVVLTLADTDYVCDY
                     IRRGGDREAFLERFKGAHSPGFDPDVHLQTVGVANQTTMLRGETEEVQRRVRQAIIDR
                     DGPELAEKNFRFFDTICGATQERQDALRELLNVRMDLLLVVGGYNSSNTSHLAEMGEE
                     KLPTYFVLNASRLASATEIKHYNLHEKREVVSHFWLPNGPAVIGITAGASCPNNLIEE
                     TLIRLFELRGISRQDLELAA"
     gene            complement(1684..>2018)
                     /locus_tag="VFY96_15870"
     CDS             complement(1684..>2018)
                     /locus_tag="VFY96_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX5608003.1"
                     /translation="ERRRQSEPDRGYPMAYWCEFKPAYGFHEGFVWPVPHTHGCVRLH
                     REAAARLFALTRIGTPVEIASSLPEDQKYGNLVRRLDQSRDPDPPRAQLMSPSWFNDP
                     PGPLLVDQ"
BASE COUNT          433 a          579 c          540 g          466 t
ORIGIN      
        1 ggcacctcct tgccgtcttt ttcagcgaca cgcttgtagt tgaccgggct tagatcgtct
       61 ttgcgcagaa gacgaaattt caattcctcc cgtcgcgtag ctggataaag cgcgataggt
      121 ctgtttacca gcccaaagct aatactcccc ttccagatcg ctcgcgccat aacaaattga
      181 gttcgtgcca cgcgccgatt ttgcgtgcgt gaagatgggg atagcacact cggatacctc
      241 attaacacct cacttcagtg aggtgcgtct acatgcgtaa ctgactaaac cgtttaaacg
      301 gttttaggat tgagccgagg cgagtacacc gggctgaagc cgcggtgtta ctgaaaagca
      361 ggtcagattc taggccgcca gctcgagatc ctggcgcgag atcccgcgta gttcgaaaag
      421 ccggatcagc gtttcttcga tcaagttatt cggacaagat gcgccggcgg taatgccgat
      481 tacagcaggc ccgttcggca accaaaaatg cgaaacgact tcgcgtttct cgtgcaaatt
      541 gtaatgcttg atttccgtag cggaagcgag ccgcgaagcg ttcagcacaa aatatgtcgg
      601 caacttctct tcacccatct cggcgaggtg cgatgtgttc gagctgttgt aaccgccaac
      661 gacgagcaac agatccatgc gaacattcag caattcccgc aacgcatcct gccgctcctg
      721 tgtggcgcca caaatcgtat cgaagaaccg gaaatttttc tcggcaagtt caggcccgtc
      781 tcgatctatg atcgcctgcc gaactcggcg ctgcacttct tcagtctccc cgcgtaacat
      841 tgtcgtttga ttcgctacgc caacggtttg caggtgcaca tcgggatcga atcctggtga
      901 atgcgcgccc ttaaatctct ccaggaacgc ctctctgtct ccaccgcgcc ggatgtaatc
      961 gcaaacgtaa tcggtgtccg ccagcgtcag gacgacgagg tagtgtccgc taccatcgcc
     1021 gagagcgcgc gaactcgtgg ctttggtctc ttcgtgctcc gccttgccgt gaatgatgga
     1081 tgtagccgat tcaattgcgt atttgcgtac tcgcttccaa acactcatta cgtccccgca
     1141 agttgtatca acgatctggc agccgcgatc cttgatctgt tgctggatcg ataattcggt
     1201 tccaaacgcc ggcacgatga cgacgtcgtc ggggccgaga tcagcgatgt ccgcttcctt
     1261 atttttaccg gtgagattct tgatacccaa cgaggcaatc tgatgattga cctccgggtt
     1321 atgaataatt tctccgacga tgaacaagcg gcgatccttg aaaaccttac gcgcggcgta
     1381 agccaggtcg attgcgcgtt cgacgccgta gcagaaacca aactgcttcg ccaggcgcac
     1441 cgtcgtatct cctacggaaa tcgttccgcc ggcgcgccga accttttcga tgatatcgct
     1501 acggtagtgt gactcgacct gttgctgcac agccgccatc acgtcgggcg tgcgcaggtt
     1561 cactttctgg cgggcagttg tgccgctgga aatcaggttc gtgctcatgg cgtcggttaa
     1621 ggtaacccta accggccgtt ttccaatggc cgcatcggcc aaaatttcct tatccggaga
     1681 cgatcactga tcgacgagca atggaccggg aggatcgtta aaccagctgg gcgacatcaa
     1741 ttgcgcgcgc ggcgggtctg ggtcgcggct ttgatcgagc cgacgaacta aattgccata
     1801 tttctgatcc tcgggcagcg agcttgcaat ttccaccggg gtgccgatcc gggtcaaggc
     1861 gaagagtcga gcagccgctt cgcgatggag gcgaacacag ccatgggtat gcggcacggg
     1921 ccacacaaat ccttcgtgaa atccataggc cggcttgaac tcgcaccagt aagccatcgg
     1981 gtaacctcgg tccggctcgc tctgccggcg gcgctctt
//
LOCUS       DASULZ010000773         3634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1410081, whole genome shotgun sequence.
ACCESSION   DASULZ010000773 DASULZ010000000
VERSION     DASULZ010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410081"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(442..1290)
                     /locus_tag="VFY96_15875"
     CDS             complement(442..1290)
                     /locus_tag="VFY96_15875"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5608004.1"
                     /translation="MSVTPLNEIIDPMAPVPIPEQVSAKEGMAALPDTNLGYWDTGGT
                     GEPVVFLHPASGSALVWLYQQPVFAQAGYRAIAYSRRNYYNSHSAPDENPGSASVDLQ
                     NFVDYLGLNKFHLVSSAAGGSVAADYALSHPERLLSLTVSSNNLAARNGYIAEAAERI
                     KLREWDDLPRWFRELGPSYRAVNPQGVAKWIELNEKSETGRGARQNLVNVVTPDKLTS
                     LKIRTLLVTGDADMFTPPSIMRMIARQLPNNILVIVPECGHSPYWEQPELFNRTVLDF
                     IRRSSK"
     gene            complement(1337..2329)
                     /locus_tag="VFY96_15880"
     CDS             complement(1337..2329)
                     /locus_tag="VFY96_15880"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5608005.1"
                     /translation="MKIKSWLICFTVILLFSPKLQGQSLPKVRAAYTSIGIQFDPVYI
                     MKELDLPRKYGLDVDVLFVPVSSRAIQAALAGEIQFITSGGVANINANVTGADFTGLT
                     ATLNTFVFKIIGSPEIKKPEMLKGKKVGISRLGGASDFSIRYALTHWGLMPDKDVAII
                     QVGGEPEEVLALQNKAIDAVILSEPFATVARRAGAALIADLSQLGVPYTMHGFGVRKI
                     FMQANRDVVIRFMKAYLEGIYVFKTDKNTALNVLKKYTRLDDLSLVQVAYDEMSQRLI
                     RRVPYPDGAGIQTIIDQLAKSRPQMKNLNPNDFIDPSILKEIEDSGFVKKLYGN"
     gene            complement(2449..2775)
                     /locus_tag="VFY96_15885"
     CDS             complement(2449..2775)
                     /locus_tag="VFY96_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608006.1"
                     /translation="MTQGFRINFTAPLLFGIFLFANGHSTAADKLRIGYSGATVSNAH
                     VMGDRRRQAVQKNGIDPQILYLQTTLGQRAMIAGEIDMCVYSGRLLAFGSIAGCRCGH
                     GGELSQ"
     gene            complement(2914..>3634)
                     /locus_tag="VFY96_15890"
     CDS             complement(2914..>3634)
                     /locus_tag="VFY96_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608007.1"
                     /translation="GGPAVMLIINGPIRKELNINGGPNALGEGWRANATIGRFVQLVK
                     RNIGGSYPGTTCKATLGWPGKYSICVGENEEASPWEPLHVERGFEREQSTVTAISADA
                     SIRASDLDSTKAVGILTNFAQKMDGPSGPEAIMVICPEHAKIIAGDGFSKKDVQKFVW
                     ERAAYRMKDLPDETFAQRVKRRSDLQLTRDSVIPVTEKPEDILVIVAGGDGSQSQYIH
                     VWGQSTPEGGSTRSVTKLIQE"
BASE COUNT          818 a         1009 c          936 g          871 t
ORIGIN      
        1 ggtggctggc tcgtgatcga caacgtaacc tcgccgcgcg tccagacaga gcttttgaac
       61 agagctgcca aagacccgcg agtagacagc gtcctcttgc cgtttcccaa gggggatctc
      121 atctgccgga agtgctaaga acaagaccgc cggttaacgc aaacatatac atgctctaac
      181 tccaatttgg agcgtgcgtc attcccgcat gcttttggcg gtggttcgac tgtgctcacc
      241 acaggcggaa ttccgactgg acacaccgat tatgcattcg cggctgacgg ttttgaggcg
      301 tttctattca cccggtatat tcgaagaact aagtttcgta gggtgcgctt tggcgcaccg
      361 cttatgcgct gcagaggtgc agaagactat aatcggagtg gacgtggttc actctcgcga
      421 tcgggtgact ttccctatga gctatttcga acttcggcgg atgaagtcga gcaccgtacg
      481 attgaagagc tcgggttgct cccaatatgg ggagtgaccg cactcgggaa caatcacaag
      541 tatattattc ggcagctggc gcgctatcat tcgcataatc gaaggcggag tgaacatatc
      601 cgcatctccg gtcacaagca gcgtccgaat tttcaggctt gtcagcttgt ctggggttac
      661 gacattcacc aggttttgtc tggcccctct gccagtttct gatttctcat tgagttcaat
      721 ccacttagcg acgccttgcg gattgacagc acgatacgac ggtccgagct cgcggaacca
      781 gcgcggcaag tcgtcccatt ccctcagctt gattctctcc gcagcttcag cgatgtagcc
      841 attccttgca gcgagattgt tactggatac tgtaagactg agtaaacgtt ctggatgaga
      901 cagcgcgtaa tccgcggcga cactcccgcc tgcagccgaa ctgacgagat gaaacttatt
      961 caggccgaga taatcaacaa agttctgcaa atctacggat gcgcttcccg gattttcgtc
     1021 gggagctgaa tgcgagttgt aataatttcg ccgcgaatag gcgatcgctc gatagccagc
     1081 ttgcgcgaac accggctgtt gataaagcca aaccaacgcg ctgcctgacg ctggatggag
     1141 aaacaccaca ggctcgcccg tgccgccggt atcccagtaa cccaggttgg tatcgggaag
     1201 cgcagccatg ccttccttag cagaaacttg ctccggaata ggcacgggcg ccattggatc
     1261 gattatttca ttcaaaggag tgacactcat cgatgacctc gagatagcgt gttcaacaca
     1321 cagtgcttaa gcgaacttag ttcccataca acttttttac aaacccgctg tcctcgatct
     1381 cctttaagat cgacgggtcg ataaagtcgt tggggttgag atttttcatt tgcggtcggc
     1441 tcttcgcgag ttgatcgatg atcgtctgaa tgcctgcgcc gtcggggtaa ggaactctgc
     1501 ggatcagtcg ttgcgacatc tcgtcatagg cgacttgaac caaagagaga tcgtccagcc
     1561 gggtatattt tttcaaaaca ttcaacgcag tattcttatc ggtcttgaag acgtaaatgc
     1621 cttccagata cgctttcatg aaccggatca ccacgtcccg gttcgcttgc ataaaaatct
     1681 tgcgcacgcc gaatccgtgc atcgtgtaag gcacgccaag ctggctcaag tcggcgatca
     1741 gcgcagcacc cgctcgcctg gcgacggttg cgaacggctc ggataaaatg accgcgtcga
     1801 tcgccttgtt ttgcagcgcc agaacttctt ccggctcgcc gcccacctgg ataatggcaa
     1861 catccttatc cggcatgagt ccccagtgag tcaatgcata ccgtatcgag aaatcactcg
     1921 cgccgccgag ccgcgaaatt cccaccttct taccttttag catctccggc tttttaatct
     1981 ccggactgcc gatgattttg aaaacaaagg tattcaacgt cgcggtgagc ccggtaaaat
     2041 cggcaccggt gacattagcg ttgatgttcg ctacgccacc actggtaata aattgaatct
     2101 cgccggcgag cgccgcctga atcgccctcg agctcaccgg tacaaataac acgtcgacat
     2161 ccaaaccgta cttccgtggt aaatcgagct ctttcataat gtaaaccgga tcgaattgga
     2221 tgccgatcga cgtgtaagcg gcccgcactt tcggcaggct ttgaccctgg agcttgggac
     2281 taaaaagtaa gatcaccgta aaacaaatga gccagctttt tattttcatc gttcttcctc
     2341 gatggactct agcggacctc gtcagtgacg atagattatc catttcttca tgactcggct
     2401 gctgatcgga aagctcgggc tagatccgga aaaggacggt acaaaggcct attgagaaag
     2461 ctcaccacca tgaccacatc ggcaccctgc aatcgagccg aaggcaagca ggcggcccga
     2521 ataaacgcac atgtcgatct cgccggcgat catcgccctc tgtccgaggg tcgtttgcag
     2581 gtagagaatc tgcgggtcga tgccgttttt ctgaacagct tgccttcgtc ggtcacccat
     2641 aacatgggca ttgctcaccg ttgccccgga atagccgatg cgcagcttat cggcggccgt
     2701 actgtgtcca ttcgcaaaga gaaaaattcc gaagagcaat ggagctgtga agttgatacg
     2761 aaagccctgt gtcatcctga agcgaaagct gaaggatctc ggccgagacc cttcgctcat
     2821 gctcagggtg acaggattcc atgattcact agaggcttcc catcttttag ccggaggagc
     2881 gcggcgaccg gaagcttcca aatattaaaa ctcttattcc tgaatgagtt tggtaacact
     2941 acgcgtcgat ccgccctccg gggtactctg tccccagacg tgaatatact gggattgcga
     3001 cccgtcaccg ccggccacga tgacgagaat gtcttccggc ttttcggtca cgggaatgac
     3061 gctgtcgcgc gtcagctgga gatcggagcg gcgtttgacc cgttgcgcaa acgtctcgtc
     3121 gggtaagtct ttcatccgat atgcggctcg ttcccaaaca aatttttgga cgtctttttt
     3181 gctgaagcca tctcctgcaa taatcttcgc atgttccggg cagatgacca tgatcgcttc
     3241 cggtcccgag ggaccgtcca ttttttgcgc gaagttggtg agaataccca cggctttagt
     3301 gctgtcgaga tccgatgccc gaattgaagc atcagcgctg attgccgtca ccgtgctttg
     3361 ctcgcgttcg aagccacgct ccacgtgcag aggctcccat gggctcgctt cttcgttttc
     3421 accaacgcag attgaatatt ttcctggcca gcccagggtc gccttgcagg tcgtgccggg
     3481 ataagagccg ccgatgttcc gtttcaccag ttgtacgaat cgaccgatgg tcgcattggc
     3541 gcgccaaccc tcgcccaagg cattgggacc gccgttgatg ttcaattcct ttcggatcgg
     3601 cccattgata atcagcatta ctgccggacc gccc
//
LOCUS       DASULZ010000774         2633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1410161, whole genome shotgun sequence.
ACCESSION   DASULZ010000774 DASULZ010000000
VERSION     DASULZ010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410161"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1297)
                     /locus_tag="VFY96_15895"
     CDS             complement(38..1297)
                     /locus_tag="VFY96_15895"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX5608008.1"
                     /translation="MEGVLFPHGSPEGREGLKEYRDRGGYQALEKTLTVPPEELIKIV
                     SDAGLRGRGGAGFSTGKKWQFTREAPEQPRYLILNGGEDEPGSKKDRVLLENLPHLVL
                     EGTILGAYAIGASKAYLYINARYDDALKSVQDALDEAKNAGYCGNNVLGSGFDLEIEI
                     VPAPHNYVAGEDTAVIEVIEGKKAWPRQKPPFPVTVGLFGKPTAVNNVETLANVPPIV
                     LKGADWYRKFGTAESTGTMIFSLNDDVNRPGVYELPFGTPLRYLIEECGGGIKGGQKI
                     KAIMPAAPSSAFLPPEKIDTPLDHNSMREAGSALGCGVVKLVAEGTCIVEELIKIADF
                     FAAESCGQCPACRMETSSLAMMLKKVQAGQGGLPMLEQFGKIIAFNKGKGFCNLIAMP
                     GPPIESAIKLFPADFQAHLTTGKCPAN"
     gene            complement(1301..1624)
                     /locus_tag="VFY96_15900"
     CDS             complement(1301..1624)
                     /locus_tag="VFY96_15900"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX5608009.1"
                     /translation="MARICLVCQNIDCKSRGSEQLTTELQQKIAAKGISDVEVKPYMC
                     FGACQEGPNIVLYPEKSWYAKVQPQDLDDIVEHLAGGPHVQRLDTIDSSLKELIYQLL
                     DTGVF"
     gene            complement(1691..>2633)
                     /locus_tag="VFY96_15905"
     CDS             complement(1691..>2633)
                     /locus_tag="VFY96_15905"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEX5608010.1"
                     /translation="WQRVTQIPNQSIRDALMNLIVYQGDTEFASVEQQKNLLDTAPTE
                     YDRQALTRVNSEEMRHGWQMCYLLVNYFGDSGKLEARKLLERRAFRGDRLLGSFNAPV
                     NNWLDFFTYTEFVDRDGKYQLTMLSHSGFAPLAESVTAMLKEEFFHMFTGHTGLTRIL
                     RAGKIPIPIVQKYFNKWLSTAYDLFGTDHSSSAHWAYTWGLKGRFDEHEAQVPAEKDK
                     LNDLARGHYMAESAKLIEQLNQLVPEGQPKLYAPDFKFNRSIGENVGKPFSVTGELLS
                     PEAYKNHLRETLPMEEDEKILADIIEGKEWVAQVQMN"
BASE COUNT          542 a          732 c          714 g          645 t
ORIGIN      
        1 atgctagccg cgggaagaac caagctgcag tatggattca gttcgccggg cacttaccgg
       61 ttgtcagatg ggcttgaaag tcggcgggga aaagttttat cgcgctctcg atcggcgggc
      121 ctggcatggc gatcaggttg caaaaacctt tgcctttgtt gaatgcgatg atcttgccga
      181 actgttcgag catcggcagg ccgccttgcc cggcctgaac ttttttcagc atcatcgcaa
      241 ggctgctggt ttccattcgg caggcagggc attggccgca cgattcggcg gcgaagaagt
      301 cggcgatttt gatcagctct tccacgatac acgtgccctc agccaccaac ttcactacac
      361 cgcaaccgag ggcggagcct gcttcgcgca tggagttatg atcgagcggc gtgtcgattt
      421 tttccggcgg cagaaaggcc gaagacggcg ctgccggcat gatcgctttt attttctggc
      481 cgccttttat cccgccaccg cattcctcga tcaagtagcg cagcggcgtg ccaaaaggca
      541 gctcgtaaac gccggggcga ttgacgtcgt cgttcaacga aaaaatcatt gtaccggtgc
      601 tctcagcagt gccaaatttc cggtaccagt cggcgccttt taaaacgatc ggcggaacgt
      661 tagccagtgt ttcgacgttg ttgaccgctg tcggtttgcc gaacaatccg acggttacgg
      721 ggaagggcgg tttctggcgc ggccaggctt ttttgccttc gatcacctca atgacggcgg
      781 tgtcctcacc agctacatag ttatgcggcg cgggaacgat ctcgatttcg aggtcgaaac
      841 cgcttccgag aacgttgtta ccgcaatacc ccgcgttctt cgcttcgtcc aatgcatcct
      901 ggacgctctt cagcgcgtcg tcgtagcggg cgttgatata gaggtaggct ttgcttgcgc
      961 cgatcgcgta tgctccgagg atcgtccctt cgaggaccag gtgaggaaga ttttccagca
     1021 gcacgcggtc ctttttgctg ccaggctcgt cttcgccgcc gttgaggatt aaatagcggg
     1081 gttgctcggg cgcctcacgc gtgaactgcc attttttgcc ggttgaaaaa ccggcgccgc
     1141 cccggccgcg caaccccgcg tccgaaacga ttttgatcag ctcttcaggc ggaaccgtca
     1201 gagttttttc taaggcctgg tagccgccgc gatcacgata ttctttcaat ccctctcttc
     1261 cctcggggct accatgcggg aaaaggacgc cttccatcac ttagaaaacc cccgtgtcga
     1321 gcagctgata gatcagttct ttcaatgagg agtcgatggt gtccagcctc tgcacatgcg
     1381 ggccgccggc aagatgctcg acaatatcgt cgagatcttg aggctggact ttggcgtacc
     1441 agcttttttc cggatacagg acgatgttcg gtccctcttg gcaagcgccg aagcacatgt
     1501 aaggtttcac ttccacgtcg ctaatgccct tggcggcgat tttttgttgg agctcggtcg
     1561 tgagctgctc agagccgcgc gatttacaat cgatgttttg gcaaaccagg caaattcgag
     1621 ccactacgtt tctccttgcg ccctaattac tcggcccgca accgagatgc aaccggacga
     1681 tctgcgcgga ctagttcatt tgcacctggg caacccactc tttaccctcg ataatatcgg
     1741 ccaggatctt ttcgtcttcc tccatgggta aagtttcccg cagatggttt ttatatgcct
     1801 ccggactcaa caattctccg gtgacactga atggcttgcc gacgttctcg ccgatggagc
     1861 gattgaattt gaaatccgga gcgtaaagct tcggttgtcc ttctgggacg agttgattga
     1921 gttgctcgat gagttttgcg ctctcagcca tatagtgacc acgcgccaaa tcgttcagct
     1981 tatccttttc ggccggaact tgcgcctcat gctcatcgaa gcgtcccttc aaaccccagg
     2041 tgtatgccca atgagccgaa gacgaatgat ccgtgccaaa caggtcatag gctgtcgaaa
     2101 gccacttgtt gaagtacttt tgcacgattg gaatcggaat cttgccggcc ctgagaatgc
     2161 gcgtgagccc cgtgtgaccg gtaaacatat ggaaaaattc ttccttgagc atcgccgtaa
     2221 cgctttcggc cagtggggca aaaccagaat gtgaaagcat cgtcagctga tatttgccgt
     2281 cgcgatcgac gaattcagta taggtgaaaa aatcgagcca gttgttgacc ggcgcgttga
     2341 acgaaccgag cagtcgatcg ccgcgaaaag cgcggcgctc cagtagtttt cgcgcctcca
     2401 gcttgccgga atcgccgaaa taatttacca gcagatagca catctgccag ccgtgccgca
     2461 tctcttcgct gttcacacgg gtcagcgctt ggcggtcata ttccgtaggc gccgtatcga
     2521 gcagattttt ctgctgctcg acggaagcga attcggtatc gccctgatag acgatcaaat
     2581 tcatcaaagc atcacgaatg gattgattcg ggatctgggt gactcgttgc cac
//
LOCUS       DASULZ010000775         2374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_799812, whole genome shotgun sequence.
ACCESSION   DASULZ010000775 DASULZ010000000
VERSION     DASULZ010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799812"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="VFY96_15910"
     CDS             complement(<1..961)
                     /locus_tag="VFY96_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX5608011.1"
                     /translation="MLRGIAVISCASLAACQTFSQDGGMSVTADIAGKILNKDIVAIR
                     SEEDVAAARVEVERLLKRPLTADAAVQIALLNNRGLQASYNELGIAEAVRVQQSLPPN
                     PHFSVTRMTGSVETEIERQIVASILALATLPMRSEIAADRFHQAQLAAALETLRVAAE
                     ARRAFYRAVAAQETVRLFAQANDAAETMTQLAKRLGETGAMNKLDQAREQAFHSDVIV
                     RLASAQQRASRERERLVRALGLWGNDLKFVLPKSLPTLPKRALTLPAVEQQAVRRRVD
                     LQIARIELDTLAKAYGLTNATRFINVLDAGYADKITKDKETGET"
     gene            complement(976..1236)
                     /locus_tag="VFY96_15915"
     CDS             complement(976..1236)
                     /locus_tag="VFY96_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608012.1"
                     /translation="MSPTLRWVWIAAMRRARHCALLISVLLVIQGCAATRSLTGPDPA
                     DSDVRVPAVAYPSVLRDRRDVLPVEPSPWTAGDPSRSGKEKP"
     gene            complement(1299..1691)
                     /locus_tag="VFY96_15920"
     CDS             complement(1299..1691)
                     /locus_tag="VFY96_15920"
                     /inference="COORDINATES: protein motif:HMM:NF022610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HEX5608013.1"
                     /translation="MLWLARKKWRRWVGLLAATFYMLTLAAPVTAVALSADPTHSHCL
                     KEVHALVSHHQSADHSDHHHSVPANPQSDQSQNCCGLFGVTAIAPTFVITVVPPVVAA
                     GIAFTVSEDLFGRGFDRIDRPPRSLQSL"
     gene            1824..2189
                     /locus_tag="VFY96_15925"
     CDS             1824..2189
                     /locus_tag="VFY96_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608014.1"
                     /translation="MLARAPLLIGILAAGVLAGCASSDDASGRFLVQPDRYQLYSCRE
                     LAEAAQTIGVRQLELEGLMAKAGPDASGRFVSTIAYRPEYLQLRGQMNEIRRTSTEKK
                     CKFNLDAAPGARVSDQVIR"
BASE COUNT          502 a          685 c          732 g          455 t
ORIGIN      
        1 cggtttcgcc ggtctcttta tccttagtaa tcttgtcggc ataaccagcg tccaggacgt
       61 tgatgaaccg ggtcgcattg gtgaggccat aggccttggc aagcgtatcg agctcgatgc
      121 gcgcaatttg caggtcgacg cggcgacgga ccgcctgctg ttccaccgca gggagggtga
      181 gagcgcgctt cggcaaggtt ggcaacgatt tcggaaggac gaacttcaaa tcgttgcccc
      241 agaggccaag cgctcgcacc agacgctcgc gctcccgcga ggcgcgctgc tgcgcgctgg
      301 cgaggcgaac aatgacgtcg gaatggaagg cctgttcgcg cgcctgatcg agcttgttca
      361 tcgcaccggt ctcgccgaga cgcttggcga gttgcgtcat cgtttcggca gcgtcgttgg
      421 cctgtgcgaa aaggcgaact gtttcttgcg cggcaacggc gcgatagaag gcgcggcgcg
      481 cctcggcggc gacgcgcagg gtttcgaggg ccgccgccaa ttgcgcctgg tgaaaccggt
      541 cggctgcaat ttcgctgcgc atcggaagcg tggcaagggc gagaatactg gcgacgatct
      601 ggcgctcgat ctctgtttcg accgagccgg tcatccgcgt tacggagaaa tgcgggttgg
      661 gtggaagcga ttgctgaacc ctgaccgcct cggcgatgcc aagctcgttg taagaggcct
      721 gcagaccgcg gttgttgagc agcgcaatct gcacggccgc atccgcggtc aacggacgct
      781 tgagcaagcg ttcgacctcg acgcgcgccg cagctacgtc ttcctccgag cggatcgcga
      841 cgatgtcctt attgagaatc ttcccggcaa tatcggccgt taccgacatg cctccgtcct
      901 gtgaaaacgt ctggcaggcc gcgagcgatg cgcagctgat caccgcaatt ccgcgcaaca
      961 tggtcaggca acgtctcacg gcttttcctt gccagatcgg gatggatcgc cggcggtcca
     1021 gggggacggc tcgaccggca ggacatcgcg ccggtcgcgg agcaccgagg gataggcaac
     1081 tgccggaacg cgaacgtccg aatcggcagg gtcagggcct gtcaatgaac gagttgcagc
     1141 gcagccctga atcacgagca acaccgagat cagcaacgcg caatgcctcg cacgccgcat
     1201 ggcagcaatc caaacccaac gcaaagtggg ggacatcaga atccctcatc tgtctgacat
     1261 tgacgacaca caacggaacg ccgccgtcgt gcgtcagctc aaagagactg cagggatctc
     1321 ggcggtcggt cgatgcggtc gaacccccgc ccgaataggt cttcggagac ggtaaatgct
     1381 atgccggcgg caacgaccgg cggcacgacg gtgatcacaa aagtcggggc gatggccgtc
     1441 acaccaaaaa ggccacagca attctggctt tggtcactct gcggatttgc gggcacagaa
     1501 tggtgatgat cggaatgatc tgcggattga tggtgactca ccagagcatg aacttctttg
     1561 agacaatgcg agtgcgtcgg atcggcagac agcgcaacag ccgtgaccgg agccgccaag
     1621 gtcagcatgt agaaagtggc ggccaacagg cccacccatc ggcgccattt cttgcgcgca
     1681 agccataaca ttgttttttc ttaccagctt cgccggattc gtaacaggcg ttaaattagc
     1741 accgaaaaca gtgatacttt agcttgctct aggtgccata tcgataccac tgcaatgcca
     1801 gaatcatgca ataggcggat ccgatgctag cccgcgcgcc cctgctgatc ggaattctcg
     1861 ctgccggcgt gcttgccggt tgcgcgtcgt ccgacgacgc gtcggggcgc tttctggttc
     1921 aacctgacag ataccaactt tatagctgca gagagcttgc cgaggcggct cagacgatcg
     1981 gcgtacgtca actggagctt gagggattga tggcgaaggc tgggccggat gcgagcggtc
     2041 gtttcgtgag cacgatcgcc tatcggccgg aatatctgca attgcgcggc cagatgaacg
     2101 aaatacgcag gacgtccacc gaaaagaaat gcaaattcaa cctggatgct gctccaggcg
     2161 cgcgcgtaag cgaccaggtc atacgctgac caaccagata gcccgctcga tggaactttt
     2221 acaggctgcg gaacttgtag ccgtggaacc cgccacggac gtggcatcac aagggggcag
     2281 gtatgggaat tctgatcagc attcttatta cttttcttgt cgttatcctg gttctctacc
     2341 taatcaatat gctgccgatc gacgggcgcg cgaa
//
LOCUS       DASULZ010000776         2581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_495729, whole genome shotgun sequence.
ACCESSION   DASULZ010000776 DASULZ010000000
VERSION     DASULZ010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495729"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1255
                     /locus_tag="VFY96_15930"
     CDS             <1..1255
                     /locus_tag="VFY96_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5608015.1"
                     /translation="LIAQGLGDVVLLGSNHCSGTLRVKEFLTRNGHPYAYIDLDRDAD
                     VQNLLDRFHVSAADVPVLICRGDVVLRNPTNREIAGSLGFNEVIDPAQIRDVVIVGAG
                     PAGLAAAVYGASEGLDVLVLETSSPGGQAGSSSKIENYLGFPTGISGQALAGRAYNQA
                     QKFGAHVMIAKDAKRLDCERMPYAVEIDDDRRVLARTVIIATGASYRKPIIDNLAHFE
                     NAGVYYGATFMEAQLCRGQDVVVVGGGNSAGQAAVFLAQTASRVYMLVRSAGLAETMS
                     RYLIRRIEENSRIVLRSKTEIVSLEGVEHLDRIRWRDETGHVEAHDIRHVFLMTGAVP
                     ATDWLDGCAVLDENGFIKTGPDLSVEDLAAARWPQARAPYLLETSLPGVFAVGDVRAG
                     NIKRVASAVGEGSIAISFVHQVLRQ"
     gene            1267..1536
                     /locus_tag="VFY96_15935"
     CDS             1267..1536
                     /locus_tag="VFY96_15935"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEX5608016.1"
                     /translation="MTAGSCAHIETIKNIKHAKRRECEECVKSGSSWVHLRICQECGA
                     TRCCDDSPNRHATQHARGSHHPVIASAEPGERWLYCYPDEAFAEY"
     gene            1674..2075
                     /locus_tag="VFY96_15940"
     CDS             1674..2075
                     /locus_tag="VFY96_15940"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5608017.1"
                     /translation="MSEADNENRVRAFYEATVPGHRESLRGLQAPHVIYDLPLGMPIG
                     CGHFEGLQDVLERFLTSFYQALDVHFIAEEFISAGDQVVALGRIEGQTRKAAVPLNVP
                     FAHVWTVRDAYLQRLRAFTDTAVIAHALEKS"
     gene            complement(2189..>2581)
                     /locus_tag="VFY96_15945"
     CDS             complement(2189..>2581)
                     /locus_tag="VFY96_15945"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5608018.1"
                     /translation="VSDLDRSMDFYTRLLGMDVQRIRPNPDKSERVGYVGYGSEDEYP
                     GLELIETGGPGRIKEMPKWHGHVAIFVSDLYKLSARLKAEGVSFTLGPQPNRPSSQDH
                     VAFIEDPDGYTVELTERHAKSGPPVKIT"
BASE COUNT          550 a          730 c          757 g          544 t
ORIGIN      
        1 gttgatcgcg caggggctcg gcgatgttgt gctccttgga tcgaaccatt gctccgggac
       61 gctccgggtc aaagagttcc tgacgcgcaa cggccatccg tacgcctaca tcgatctcga
      121 tcgagacgcc gacgtgcaaa atctgctgga ccgctttcat gtgagcgccg cggacgtgcc
      181 cgtgctcatc tgtcgcggcg acgtggtcct gcgcaacccc accaatcgcg agatcgctgg
      241 atctctcggg ttcaacgaag tgatcgaccc ggcgcaaata cgcgacgtgg tcatcgtcgg
      301 cgccggaccg gcaggactgg cggcggcggt ctacggcgcg tccgaaggcc tcgatgtgct
      361 ggtactggag accagctctc ccggaggaca ggctggctcg agctcgaaaa tcgagaacta
      421 tcttggtttt cccaccggca tctccggtca ggcgctggct ggacgcgctt acaaccaggc
      481 gcagaagttc ggggcgcacg taatgatcgc gaaagacgcc aaacgtctcg attgcgagcg
      541 catgccgtac gcggtggaaa tcgacgacga tcggcgcgtg ttagcgcgga ccgtcatcat
      601 cgcaacgggc gcgagctacc gcaagccgat tatcgataac ctggcgcact tcgaaaatgc
      661 cggcgtctac tacggcgcaa ccttcatgga agcgcaattg tgccgcggcc aagatgttgt
      721 cgtcgtgggc ggaggcaact cggcgggaca ggctgcggtg tttttggcgc agaccgcaag
      781 ccgggtatac atgctcgtgc gttccgcggg cctggcggag accatgtctc gttatctcat
      841 ccgtcgcatc gaagaaaatt ccagaatcgt gctcagaagt aaaactgaaa ttgtttcact
      901 cgaaggcgtc gagcatctcg accgcattcg ctggcgcgat gagaccggcc acgtcgaagc
      961 tcacgacatt cgccatgtct ttctaatgac cggcgcagtg ccggctaccg attggctcga
     1021 tggctgtgcc gtgcttgacg agaatggttt tatcaagacg ggaccagatc tgtccgtaga
     1081 ggacctagcg gcagcgcgct ggccccaagc ccgcgcgcct tatctcctgg aaacgagtct
     1141 gccgggagtt tttgcagtcg gcgatgtgcg cgccggcaac atcaagcggg tggcatcggc
     1201 agttggagaa ggttccatag cgatctcgtt cgttcatcag gtcctgcgcc aataggagag
     1261 agttaaatga cagccgggag ttgcgcgcat atcgagacga tcaaaaacat caaacacgcg
     1321 aagcgccgcg aatgcgagga atgcgtgaaa agcggttctt catgggtcca tctccggatc
     1381 tgtcaggaat gcggagctac ccgctgttgt gacgactcgc cgaaccgcca cgcgacgcaa
     1441 catgcgcgcg ggagccatca tccagtaatc gcctcggcgg agccggggga acgctggttg
     1501 tactgctatc cggacgaggc ttttgcggaa tactaagcga cagagacttt cgttcaacac
     1561 ttgccgtgac aaactcaaga acagtcatga cgaggcgatg gctcaagatt gtttttcaat
     1621 gcgcaggaga ataacttgcc gcgcccggaa tgaactcgac aggagaaaaa acaatgagtg
     1681 aagcagataa tgagaaccgc gtccgagcat tttacgaagc gaccgtgccg gggcatcgag
     1741 agtctctgcg aggcctgcaa gcaccgcacg tcatttacga tctgccgctg ggtatgccga
     1801 ttggctgcgg ccatttcgag ggccttcagg atgtgcttga acggtttctc acaagttttt
     1861 atcaagccct cgatgttcat tttattgcag aggagttcat ctccgcaggc gatcaagttg
     1921 tcgcgctggg ccgaatcgaa ggacaaaccc gaaaggctgc agtccctctc aatgtgcctt
     1981 tcgcgcacgt ttggacagtc cgtgacgctt atctccagcg attgcgcgct ttcacggata
     2041 cagcggtgat cgcgcatgcg ctggaaaaga gctgaatggc tcgaactgtt tttcagcctg
     2101 ttcccttgca gaatgcgaca agcgcttcgc ggtgcagatt ttgagcaaga gacaacgtcc
     2161 cggattcccg aacagagaat gctggcgctt acgttatttt gactggtgga ccgctcttcg
     2221 cgtgacgctc ggtgagctcc acggtatagc cgtccggatc ttcaatgaac gcgacatgat
     2281 cttgactgct gggccgattg ggttgcgggc caagggtgaa gctcactcct tcggctttca
     2341 atctcgcgct cagcttgtag agatcggaaa caaagatggc cacgtggccg tgccatttcg
     2401 gcatttcttt tatgcgtccc ggaccacccg tctcaatcaa ttccaacccc ggatactcgt
     2461 cttcgctacc ataacctaca taaccgacgc gctcgctctt gtcgggattt ggccgaatgc
     2521 gctgcacatc cattcccagc agccgcgtgt agaaatccat gcttcggtca agatcgctga
     2581 c
//
LOCUS       DASULZ010000777         4042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1003314, whole genome shotgun sequence.
ACCESSION   DASULZ010000777 DASULZ010000000
VERSION     DASULZ010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003314"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(375..1382)
                     /locus_tag="VFY96_15950"
     CDS             complement(375..1382)
                     /locus_tag="VFY96_15950"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5608019.1"
                     /translation="MKRLHLSIVALAATVVSLVALSANLFGAESGKGTPLRLAYSAIT
                     VNQAIPWIAHDAGHFKKHGLDVELVHASSTLALQALLAGEVSIAQSVTDACVSSNLNG
                     ADTLFMGAILDKPLYSFIVNPKIKSPQDLKGKRVGVTRIGSTPDALARAMLKMWNLDP
                     ATDVTIVQLNEMGLLVQGLVNGVIDAAPISIPSNIRAKNLGYTELFDMTKIGKTYITG
                     TVVTRKRFIDGQHDTAKRFMRGFLEGMKTYLEDEDFSIKVIQKWTRTKNRDEVKEAYA
                     LQAKNMLRIPRTSIEGVKTILEGMEKLPGAKTADPRRFIDFTILDELEKEGFLKTLYK
                     N"
     gene            1654..2853
                     /locus_tag="VFY96_15955"
     CDS             1654..2853
                     /locus_tag="VFY96_15955"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5608020.1"
                     /translation="MNYKCISADSHLEIRPDRYTGRVPQRFRDRAPKVITLEDGTLAV
                     LQEGQPLERLISNISCGVPYEERRPFDPLPGEHYEKSPGTGSPEQRLREQDKDGVDAE
                     VLFPGNVGPGFWRGIRNDEAYKAVVRAYNDWLAEEYCSYAPERLIGIGVIPITNINDA
                     MAELSHCKDMGLKAVAIDNFPAGYRYPTLDDDRFWAAAVDLEMALTIHVEFGFPRRGR
                     GQPAAAALPSFKYARQPDPEHHVPDVIERFNKYGFRGSKQVVQMIWGGAFERFAKLKI
                     YVAEVQIGWVPNWMDQMDNEYGRQQYWAERVLGLPRLKRMPSEYAREHCYWGFNRNPV
                     GVRIARHEMDIDKLMWASDFPHLESDWPNSRKVIAENFAGSSEDEIWKMTVGNAVEYF
                     HLNDDRR"
     gene            2896..3900
                     /locus_tag="VFY96_15960"
     CDS             2896..3900
                     /locus_tag="VFY96_15960"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5608021.1"
                     /translation="MSDLKALDAELAKDSMRGLWAREEAIRREPVPFGKPMLWKWAKI
                     RAGLEAAGQLITTNYKGARRAISLVHPNMGDSTSHTLNMAVQLVKAGEAVYSHRHTNA
                     AMRFVIEGGEGVYTITNGEKCVMERGDLVIQPSWGWHNHINETDHDAIWIDCLDSGLM
                     RMLRTMFQEPHPAEDVRLYNSPVDTAVRNPGLLAPPGQPLKSLVYKWGEVRKALAAML
                     AESENAFDGKCLEYRNPVTGGSTFPTFSCWIQMLDGAKQTQEHRHTSNQLYYVVEGSG
                     ITEVAGENLAWESGDFFVVPNWSWHRHENSSSTVPAVLFSTTDRPLQEAIGVYREEAR
                     "
BASE COUNT          920 a         1062 c         1141 g          919 t
ORIGIN      
        1 gttagcggac caagtactga actaaattgt aacgtcatgt tcgtctgaat aaacgtgtaa
       61 cggaaaagtc gcttcgaacc cgtcacaaat cccctattga agacaaaatt acaatttgtt
      121 gccgtggtta tttgaacatc gctgcctttg cactgggatt atccctcttt gaaaaagagg
      181 gataagggag attttgtcgt agtagcaggg cccacattac cttcattcct cttgcgattc
      241 ccgtagacac acgcgtccca acttggcgca tcggtgtgaa tttacggtta gattcttcac
      301 ttcgttcaga atgacactcc tgctgctatg acgctctcga aacagtgtca tgattggtac
      361 aatttcgttt acgttcagtt cttgtacagc gtcttcagaa aaccttcctt ttccaactcg
      421 tcgagaatcg tgaaatcgat gaatcgtcta ggatcggcgg tcttggcgcc ggggagcttc
      481 tccatccctt cgagaatcgt ttttactccc tcgatggagg tgcgcggtat gcgcagcatg
      541 tttttcgcct ggagcgcgta agcttccttc acttcgtcgc gattctttgt gcgagtccat
      601 ttttgaatca ccttgatgct gaagtcttcg tcttccagat aagtcttcat gccttcgaga
      661 aacccgcgca tgaagcgctt ggccgtgtcg tgctgaccat caatgaagcg cttgcgcgtg
      721 actacggttc cggtgatgta agtcttgccg attttcgtca tatcgaacag ctcggtgtag
      781 ccgagatttt tcgcgcgaat attgcttggg atcgagatcg gcgcggcgtc gatcacgcca
      841 ttcactagac cttgaaccaa cagtcccatt tcgttcaatt gcacaatggt cacgtcggtg
      901 gcgggatcga gattccacat tttgagcatc gctcgcgcga gcgcgtcggg tgtggagccg
      961 atgcgcgtga ccccgacccg tttgcctttg agatcctgcg gggatttgat tttcggattg
     1021 acgatgaaac tgtaaagcgg cttgtcgagg atcgctccca tgaacagagt atccgcgccg
     1081 ttcagattcg agctgacgca cgcgtctgtg accgactgag caatcgagac ttctccggcg
     1141 agcagtgcct gaagcgccag cgtgctactg gcgtgaacga gctcgacatc aaggccgtgc
     1201 tttttgaaat ggccggcgtc gtgcgcaatc catggaatgg cctgattaac ggtgatcgcg
     1261 ctgtacgcga gccgcagcgg agtcccttta ccggattcgg caccgaacaa attggccgag
     1321 agggcaacga gagaaacgac cgtggccgcc aatgcaacga tgctaagatg aagtcttttc
     1381 ataatcgatt ctcaaagtga tatccgttaa aaatccctcc taactccgtt tatgaaaggg
     1441 aggaatgtta gatgccatcg cgaatgtttc aagtagccgg acgtcgctgc gttcataaca
     1501 ttgaggttta tggcaggtca aatgagggtt ccatttagga aaagattttt cgctgcgctc
     1561 gaaatgacag gtatgcgatc tgcgttcgag cctggcgttg acaagttttt tctccgggta
     1621 taaatcgagc cctggttcag gtattgaggt cgtatgaact acaaatgcat ttccgcagat
     1681 tcgcatctgg agatccgacc ggatcgctat actggacgcg tgccgcaaag atttcgcgac
     1741 cgcgcgccga aagtaatcac gctcgaagac gggacactgg cggtgctgca agaagggcag
     1801 ccgctggaac ggctgatctc gaacatttcc tgcggggttc cgtatgaaga gcggcgcccc
     1861 ttcgatccgc tacccggtga acattatgaa aaatcgcccg gaaccggctc gcctgagcaa
     1921 cggctgcgcg aacaagacaa ggacggcgtt gatgccgaag tgttgtttcc cggcaacgtc
     1981 gggccgggtt tctggcgcgg catccgcaac gacgaagcct acaaagccgt cgtgcgtgcc
     2041 tacaacgatt ggctggccga ggagtattgc tcgtacgctc ccgagcggct gattggcatc
     2101 ggtgtcattc cgatcaccaa tattaatgac gcgatggccg agctgagtca ctgcaaagat
     2161 atggggctta aagccgtcgc gatcgataac tttcctgccg gttatcgcta tccgacgttg
     2221 gatgacgacc gtttctgggc cgctgcggtc gatctcgaaa tggcgctgac catccatgtg
     2281 gagttcggtt ttccgcggcg cggacgcggc cagcccgcag ccgcggcgct tccgtcattc
     2341 aaatatgcgc gccaacccga tcccgaacat cacgtgcccg acgtgattga gcggttcaac
     2401 aaatacggct ttcgcggcag caagcaagtg gtgcagatga tctggggcgg cgccttcgag
     2461 cggtttgcga aacttaaaat ctacgttgcc gaggtacaaa tcggttgggt tcccaactgg
     2521 atggatcaga tggacaacga gtatggccgt cagcaatact gggccgaacg cgtgctgggc
     2581 ttgccgcgtt tgaaaaggat gccaagcgaa tacgcccgcg agcattgcta ctggggcttt
     2641 aaccgcaatc cggtcggtgt gcgtatcgcg cgccacgaga tggacatcga caaactgatg
     2701 tgggcgagcg attttccgca cctcgaatca gattggccca attcacgcaa agtcattgcg
     2761 gaaaatttcg ccggctccag cgaagacgaa atctggaaaa tgaccgtcgg caacgccgtg
     2821 gaatatttcc atctgaatga tgaccgccgg tagggcggcc ggtggtcgtc cgaatgatgg
     2881 cgagagggca aaatcgtgtc cgaccttaaa gcgctcgatg ccgaattggc gaaagattcc
     2941 atgcgcgggc tctgggcgcg tgaagaagcg atccgccgtg agccggtgcc gttcggtaaa
     3001 ccgatgctct ggaaatgggc gaagatccgc gccggcctcg aagcggccgg ccagttgatt
     3061 acaaccaact acaaaggcgc gcgccgcgcc atcagcctgg ttcacccaaa catgggcgac
     3121 agcacgagtc atacgttgaa catggcggtg cagctggtca aagccggaga agcagtctat
     3181 tcgcatcggc atacgaacgc ggcgatgcgt ttcgtcatcg aaggtggcga gggcgtctac
     3241 accatcacca acggcgagaa gtgcgtgatg gaacgaggcg atctagtgat tcagccgagc
     3301 tggggctggc acaatcacat caacgagact gatcatgatg cgatctggat cgattgcctc
     3361 gactccggtt tgatgcgcat gctgcgcaca atgtttcaag aaccgcatcc ggccgaggat
     3421 gtgcggctct acaacagtcc ggtcgataca gcggtgcgca atcccggttt gctcgcgccg
     3481 cccggacaac cgctcaagtc gctggtttac aaatggggcg aggtgcgaaa ggctctggca
     3541 gcgatgcttg cagaaagcga aaacgctttc gacggcaagt gtctcgaata ccgaaatccc
     3601 gtcaccggcg gttcgacgtt tccgacgttt tcctgttgga tccagatgct tgatggcgct
     3661 aagcaaactc aagagcaccg ccacacctcg aatcaactct actacgtcgt cgaaggcagt
     3721 ggcataaccg aggtcgccgg agaaaacctg gcatgggagt caggcgactt ttttgtcgtg
     3781 ccgaactgga gctggcaccg gcacgagaat tcgtcttcaa cggttcctgc cgtgcttttc
     3841 tcaacgacgg atcgaccact gcaggaagca atcggagtct atcgagaaga ggcgcggtaa
     3901 gatatttttg tcgaaaccga gtccgagcac gaagaaatat acgctccctc acgagcaagt
     3961 ttgtcgcacc aaaacgtaga ctttgtcatg ctgagtggca gcgaagcatc tcgactttgc
     4021 agctattggc aagtactaga tc
//
LOCUS       DASULZ010000778         2396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1410822, whole genome shotgun sequence.
ACCESSION   DASULZ010000778 DASULZ010000000
VERSION     DASULZ010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410822"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VFY96_15965"
     CDS             complement(<1..630)
                     /locus_tag="VFY96_15965"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX5608022.1"
                     /translation="MANKSEKIAVLGAGTMGAGIAQVSAQAGYETLVYDVKQEFIDAG
                     LQRVRNFLEGSRTRGRLSVEQEQEILDRFRTTTSLEDCRGAALVIEAAPEKLDLKRDI
                     FKQLDSICAPETLLASNTSSFSITAIAAAAQHPERVLGLHFFNPPPLMALVEVIQGDR
                     TLPSIIEQATALMRSMGKTPARAKDTPGFIVNRVARPFYNEGLRVLGDGD"
     gene            complement(698..1477)
                     /locus_tag="VFY96_15970"
     CDS             complement(698..1477)
                     /locus_tag="VFY96_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017251574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5608023.1"
                     /translation="MAYETIIYEKQNGVATITLNRPQSLNAFIPQMNQEVLQALKDSE
                     RDQETRCVMITGAGRAFCAGQDLKGRTPEQKGSLGASLREKYNPMIRQIRQMEKIVIA
                     AVNGVAAGAGCNFALACDLRIASEEAKFIQSFVRVGLAPDSGGSFILPRLVGLSKAME
                     LLLLGDTVDAKEAQRIGLVARVFPVAEFAGSARSIAEQVARAPRGIGLIKRAVNRANL
                     AHLEQDLEYEAQLQEIAGRSADYDEGVKAFLEKRTPVFTGK"
     gene            complement(1693..>2396)
                     /locus_tag="VFY96_15975"
     CDS             complement(1693..>2396)
                     /locus_tag="VFY96_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5608024.1"
                     /translation="LNALSYALVKDLSLAMQELDEDNDVRVIIVTGGEKVFAAGADIK
                     EMAERGPFDERLQERLAYRDKINRIRKPVIAAVSGFALGGGCELAMSCDIIIASETAR
                     FGQPEVNLGIIPGSGGTQRLAHVLGKHRAMELVLTGDMITAADAERFGLVNRVFPVEL
                     FLEEAKHVARKIAAKPVLAVRAAKEAVLKSANTALDEGLEFERKSFYLLFASEDKSEG
                     MKAFLEKRKPEFKGR"
BASE COUNT          494 a          696 c          656 g          550 t
ORIGIN      
        1 atcgccatcg ccaagaacac gcagcccttc gttataaaac ggccgggcga cgcggttgac
       61 gatgaatccc ggcgtgtcct tggcgcgagc cggagttttg cccatgctcc gcatcagcgc
      121 agtggcctgc tcgatgatcg acggtaaggt ccggtcgcct tgaatcacct ccaccaaggc
      181 catgagtggc gggggattaa agaaatgcaa cccgagaacg cgctctggat gctgtgcagc
      241 ggccgcgatg gccgtgatgc tgaatgacga cgtattcgac gctaagagag tctccggtgc
      301 gcagatcgaa tcgagctgct taaaaatatc gcgcttgagg tcgagttttt ccggcgccgc
      361 ctcgatcacg agcgcggcac cgcggcaatc ctcgaggctg gtcgtagtcc gaaagcgatc
      421 gagaatttcc tgttcctgct cgacgctcag tcggccgcgg gtgcggctgc cttccaagaa
      481 attccgtacg cgttgcagtc cggcgtcgat aaactcctgc ttgacgtcgt aaactaacgt
      541 ttcgtaacct gcctgagccg agacttgcgc gataccggca cccatggtgc cggcgccaag
      601 cacggcgatt ttttcagatt tattagccat ggcctttccc ggtttctcgt ttctagtttc
      661 cagtttggac cgaaacgcag aaaccaaaga ccagtaacta tttccctgtg aacaccggcg
      721 tgcgtttttc caaaaacgct tttacgccct catcgtagtc ggcactacgc ccggcaatct
      781 cttggagctg cgcctcgtat tcgaggtctt gttcgagatg cgccagattg gcgcgattaa
      841 ccgcacgctt gatcaaaccg atgccgcgcg gagcacgagc cacctgttcc gcaatgctgc
      901 gcgcggagcc ggcaaattcc gcgacgggaa aaacgcgcgc cacgagcccg atccgctgcg
      961 cttctttggc atcgaccgta tcgccgagaa gcaaaagctc catcgctttg ctgagcccca
     1021 ctagccgcgg gagaatgaag ctgccgccag aatccggcgc gagaccgacg cgtacgaatg
     1081 actgaataaa tttggcctcc tcggatgcaa tacggagatc acaagcgagt gcaaaattgc
     1141 agccggcacc cgcggccacg ccattgaccg cggcaatgac gatcttttcc atttggcgga
     1201 tctggcggat catcggattg tatttctcac ggagcgaagc gccgagcgag cctttttgct
     1261 ccggtgttct acccttcaag tcttggccgg cacaaaatgc ccggcccgcg ccggtgatca
     1321 tcacgcaacg cgtttcctgg tcgcgctcgc tgtccttcaa agcttgcagc acttcctgat
     1381 tcatctgcgg aatgaaagcg ttgagcgact gcggccggtt gagcgttatt gtcgcgacgc
     1441 cgttctgttt ttcgtaaatg atggtttcgt aggccacggc tacttcactc tctggcgctc
     1501 agtttaaaag ttcgcccgaa tcagcactgg ggtccaaatc aaaaaatctg ggcgcaggct
     1561 attttgctga ttgttctatg taaactgtca ttttgaggcg aagccgaaga atctgcgcgt
     1621 gtgtgtggaa cggaaagatg cttcgctgcg ctcagcatga catgtttgta ttattcctga
     1681 ttacgccaaa gcttagcgtc ctttgaactc gggtttacgt ttttccagaa aagccttcat
     1741 gccttcgctt ttgtcctcgg aagcgaaaag caggtagaac gattttcgct cgaactccaa
     1801 accttcatcg agggccgtat tggcggattt gagcacggct tctttcgccg ctctcacggc
     1861 tagaaccggt ttagcggcga tttttctcgc gacgtgcttg gcttcttcca gaaatagctc
     1921 caccgggaac acgcggttca ccagaccgaa acgttcggcg tcggcagcag tgatcatgtc
     1981 gccggtcagc accaattcca tggcgcgatg tttaccaagc acatgagcaa ggcgctgtgt
     2041 cccgccgctc ccgggaataa tgccgagatt tacttcgggc tggccgaagc gcgccgtctc
     2101 cgaagcaatg atgatgtcgc agctcattgc gagctcacag ccgccgccga gagcgaaacc
     2161 gctgaccgcg gcgatcactg gtttgcgaat tcggttgatc ttatcccgat acgccagccg
     2221 ctcctggagt ctttcgtcga atggcccgcg ctctgccatt tccttgatat cggcgcctgc
     2281 ggcaaaaact ttttcgccgc cggtaacaat gatgacgcgc acatcgttgt cttcgtcgag
     2341 ttcctgcatc gccaacgaga gatccttgac cagagcgtag gagagcgcat tgagtg
//
LOCUS       DASULZ010000779         3479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_903365, whole genome shotgun sequence.
ACCESSION   DASULZ010000779 DASULZ010000000
VERSION     DASULZ010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903365"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VFY96_15980"
     CDS             complement(<1..607)
                     /locus_tag="VFY96_15980"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5608025.1"
                     /translation="MKRFALSLVLSLLLTGLVPAIGSAKIVVGLSSVNVAFLPVYVTE
                     AKGFFKDEGLDVLLVLFNAGATNLQALIGGDVQIMGSAFVETIGGRAAGIDVKNFWGI
                     CNLMPFQLYSQPDFKSMKQAKGKRFAISRFGSLTDFLTRATLRHFGVDEKEVTILQIG
                     STPARFAALSAKGVDASIVWFPVTEIAKSQGFNKLLDLKEIF"
     gene            complement(644..1651)
                     /locus_tag="VFY96_15985"
     CDS             complement(644..1651)
                     /locus_tag="VFY96_15985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5608026.1"
                     /translation="MKFCLQLNPQVSIKKPGASLMPSLVEQVRVADAVGFDAFSMGDH
                     YNIPGLQRLNQVPALARLCAEVKHCSVGTAVMLLGLRHPVTVASELASLDVLNEGKSF
                     AAFGLGYREEELNAFNLTKTQRYRRFVEGVEIVKKLWTEDNVSFEGKAFQLKNVTVDP
                     KPLQKPRPPIWIAANTDAAVRRAASIGDGWLIGPHSAIMELETQVQLCRSAWENAGKS
                     GSPALPIIRETFVAARKKDAVEKARPCLEQLYRAIYIKWKQNEAMNDPKELSWAFDKL
                     AKGRFILGTPSECIDQIREYEDRLGVSYMLPRFDWTPGLPQEEILASMQLFGEKVIQK
                     L"
     gene            complement(1699..2682)
                     /locus_tag="VFY96_15990"
     CDS             complement(1699..2682)
                     /locus_tag="VFY96_15990"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5608027.1"
                     /translation="MRIFSVWMLLLLIRTAVVANAEPLRVGIPGLSAEFAPVWAANDR
                     GFLKKYGFESEIITMQGGTQLAQAVIAGSIPIAVMGGAYLSAAVRGADLVMIATHMDK
                     LPYSLIVKPNLKRVEDLKGSRLAISRFGSSSDAGLRLALQKLGVNPEKEVIIVQVGGQ
                     TERFAALKANTVDGTVVIPPLSGAAQRLGYNALINMNKLGIPYPQEGVVVSRQLLASR
                     RDMVTRFLKAYIEGVKELKNDKEFAIGILAKHLRMDRKKDREALEDSFQEVVIEQMQK
                     VPGINIEAIKVGLDLLGKDKPPTASNNPRDYVDGSLMQELVKSGFVDQLYK"
     gene            complement(2749..>3479)
                     /locus_tag="VFY96_15995"
     CDS             complement(2749..>3479)
                     /locus_tag="VFY96_15995"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5608028.1"
                     /translation="STGASRSTTVMGSAVKAAAADLRRQLTAAAAEILRVNESTITLS
                     NGQAVSGDKQITYGGIVSAFFGMPGGELIGRGYMRPDGTLGKTLPLFWETGMGSVEIA
                     VDEETGTIQIEKYVTVADVGRALNPLQAEGQDEGAAIQGLGNTLFEALEYENGQPLNA
                     TLVDYRVPRFTDLPAMFSSALVENSDGPGPYGAKGMGESGIVSVAAAVGNALFNATGV
                     RIRDLPLTPERVWRALRDHRRGQH"
BASE COUNT          762 a         1030 c          893 g          794 t
ORIGIN      
        1 gaaagatttc tttaaggtcg agaagcttat tgaacccttg agacttggcg atttcagtta
       61 cgggaaacca gacgatcgac gcgtcgacgc ctttggcgct caaagcagca aaccgcgcag
      121 gcgtgctgcc gatctggaga atcgtcactt ctttttcgtc gacgccgaaa tgacgcaggg
      181 tcgcacgcgt caaaaaatct gtgagcgagc cgaaacggct gatggcaaag cgcttgcctt
      241 tggcctgctt catcgatttg aagtccggct gcgaatagag ctgaaagggc atgagattgc
      301 agattcccca aaaatttttc acgtcgattc ccgcagcccg accgccgatg gtctcgacaa
      361 aggcgctgcc catgatctgc acgtctccac cgatcagggc ctgcaaattt gtcgcgcccg
      421 cgttgaacag cacgagcaaa acatcaagtc cctcatcttt gaaaaaacct ttggcctcgg
      481 tgacatacac cggcagaaat gccacgttga cggaagaaag accgacaact attttggcgg
      541 agccgatcgc gggaacgagc ccagtcagca gcaaactcaa aacaagcgat agcgcgaatc
      601 ttttcatgac cgacctcctt aagaaagatt acagcaagaa agattacagc ttctgaatca
      661 ctttttcgcc gaacagctgc atggaagcga gaatctcctc ttgcggcaac ccaggtgtcc
      721 aatcaaaacg gggcagcatg taagaaaccc cgagacgatc ctcgtactcc cgaatctgat
      781 cgatacattc cgagggcgtc ccgaggatga agcggccctt agcaagcttg tcgaacgccc
      841 agctcagctc cttcggatcg ttcatcgcct cgttctgctt ccatttgata tagatagctc
      901 gataaagctg ctcgagacag gggcgcgctt tctccacggc gtcttttttc ctcgccgcaa
      961 cgaaggtctc gcggatgatc ggtagcgccg gggagcccga cttgccggca ttctcccagg
     1021 cgcttcggca caattgcacc tgtgtttcca actccatgat ggcggaatgg ggaccgatca
     1081 gccaaccatc gccgatggaa gcggcacgcc ttacggccgc gtcggtgtta gccgcgatcc
     1141 agatcggcgg ccgcggtttt tgtagaggct tgggatcgac agtgacattc ttgagctgga
     1201 aggcttttcc ttcgaagctc acgttgtcct cggtccaaag ttttttgacg atctccacgc
     1261 cttcgacgaa tcgccggtag cgctgagttt tagtcaggtt gaatgcgttc agttcttcct
     1321 cgcgataccc gaggccaaac gcagcgaaag acttaccctc gttgagcacg tcgagactcg
     1381 ccaactcact cgcaacagtt acgggatggc gcaggccaag aagcatgacg gcggttccca
     1441 cggaacagtg cttcacttcc gcacacaaac gcgccagcgc cggcacctgg ttcagtcgct
     1501 gcaacccggg aatgttgtaa tggtcgccca tcgaaaatgc gtcgaagccc accgcatccg
     1561 cgacgcgcac ctgttcgact aaactcggca tcaatgacgc ccccggcttt ttaatcgaga
     1621 cttgcggatt gagctgcagg cagaacttca taatgaatgc tcgagcgggt aattctcgtg
     1681 ttgggcccgg ccgccagctc acttgtagag ctggtcgacg aatccgctct tcaccaactc
     1741 ctgcatcaga ctgccgtcca cataatcgcg cggattgttt gatgccgttg gtggtttgtc
     1801 tttgccgagc aaatcgaggc ccactttgat cgcctcgata ttgatgccgg gaactttctg
     1861 catttgctcg atgaccactt cctgaaagct gtcctccaga gcttcgcggt cttttttacg
     1921 gtccatccgg agatgcttgg ctaaaatacc gatggcgaat tccttatcat tcttcaattc
     1981 ctttacacct tcaatgtagg ccttgagaaa tcgcgtcacc atgtcgcgcc ggcttgcgag
     2041 caattggcgg cttaccacga caccttcctg ggggtaagga atgcccagct tgttcatgtt
     2101 aataagcgca ttgtagccga ggcgttgggc ggcgccgctc aacggcggta tgaccacagt
     2161 cccatcgaca gtgttggcct tcagcgcagc gaagcgctcg gtttgtccgc cgacctggac
     2221 aataatgacc tccttttctg gattgacccc aagcttttgt aacgccaggc gcaatccggc
     2281 atcgctcgag gagccaaatc ggctgatcgc cagccggctt cccttcaggt cttcgactcg
     2341 ctttaagttt ggtttcacga tcaaactata aggcagtttg tccatgtggg tggcaatcat
     2401 caccagatct gctccgcgaa cggccgcgct tagatacgct ccgcccatca ccgcaatcgg
     2461 aatacttcca gcgataactg cctgcgccag ttgagtccct ccttgcatgg taatgatttc
     2521 gctttcgaag ccgtactttt ttaaaaagcc tcggtcgttg gctgcccaga ccggcgcgaa
     2581 ctcagcgctc aacccgggga tgccgacgcg caaaggctcg gcgttggcaa caacggcagt
     2641 tctgatcagt agaagcaaca tccaaactga aaaaattcgc atcaaacatc ctccttccgc
     2701 cgccccgttc tccatgtcga aacattgttc tggatccgat tacacatgtt agtgctgtcc
     2761 gcgtctatga tcccgcaaag ctctccacac ccgctccggc gtgagtggta aatcgcgaat
     2821 gcgtacgccg gtggcattga acagcgcgtt tccgaccgcg gccgccacag aaacgattcc
     2881 cgactcaccc attcctttcg caccgtaggg accgggcccg tcagagttct caaccagcgc
     2941 tgaactaaac attgccggta gatcggtaaa gcgcggtacg cgataatcga ccagggtcgc
     3001 attcagcggc tggccgtttt cgtattccag agcttcgaat agcgtattgc ccaggccctg
     3061 aatcgcagcg ccttcatctt gtccttccgc ctgaagcgga ttgagtgccc tgccgacatc
     3121 cgcaacggta acgtactttt ctatttggat cgtgcccgtt tcctcatcca cggcaatttc
     3181 cacgctgccc atcccggttt cccaaaaaag cggtaaggtt ttgccgaggg tgccgtcagg
     3241 tcgcatgtag ccgcggccga tcaattctcc gccaggcatg ccaaaaaaag cactgacgat
     3301 gccgccgtaa gttatctgtt tgtcgccgct tacggcttgt ccgttcgata aggtaattgt
     3361 gctctcattg actctcaaaa tttccgccgc agctgcggtt agctggcggc gcagatcggc
     3421 agcagcagcc ttgacggcgc ttcccatgac ggtggtcgaa cggcttgccc ccgtggaac
//
LOCUS       DASULZ010000780         3669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1207400, whole genome shotgun sequence.
ACCESSION   DASULZ010000780 DASULZ010000000
VERSION     DASULZ010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207400"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..859)
                     /locus_tag="VFY96_16000"
     CDS             complement(50..859)
                     /locus_tag="VFY96_16000"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX5608029.1"
                     /translation="MHAAGRTDVHWPNALEILQGGLAEVRISGATLAAVEGRVGQTQV
                     YFQQSGGSSFTGLVGADIEAKPGISKLLLLATTRRTIYERKVPVHVKTKAFRTEAFSV
                     PSSFDQVSPETAEEISREQADFARAFAFSSPERLWEAPFVRPVPQEASASSFGMRRII
                     NGSPRAPHSGTDLSAPFGTEVVASNYGRVVLIGNYFFAGGSVVLDHGGGLFTMYFHLS
                     ELKVAQGSSVKRGDIIGLSGASGRVTGPHLHWGARLSGARINPLELLKIFS"
     gene            complement(918..2156)
                     /gene="xseA"
                     /locus_tag="VFY96_16005"
     CDS             complement(918..2156)
                     /gene="xseA"
                     /locus_tag="VFY96_16005"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX5608030.1"
                     /translation="MVRVLNDEADLPLFAAAIRRFLSVSELNELIKGSLESHLDTVWV
                     QGEISNFRVPPSGHCYFTLKDDRSQICAVMFRRQGLSLRFTPENGMAVLCFGRVSVYT
                     VRGDLQLYVEEIEPQGQGALYIAFEQLKKKLLADGLFAEERKRPLPFLPKTIGIVTSD
                     GGAALRDMLRIIDDRFPDRRIIIRPVKVQGDGAGREIARGIHDLNRLGTVDVIIVGRG
                     GGSLEDLWAFNEEVVARAIFASNVPVVSAVGHEIDFTIADFVADCRAPTPTAAAEMIV
                     PRKLDLREQVDLLELQLRRAVDLALASVRETLRGLLKRLADPSRKLRDHQQRLDDLSI
                     DLLHRFQERLRKNKNDLSECAARLNALSPLAVLERGYAIAHKLPEEQIVKRADALTVG
                     DRLRITFSQGKSVCRVEKNE"
     gene            complement(2244..3596)
                     /locus_tag="VFY96_16010"
     CDS             complement(2244..3596)
                     /locus_tag="VFY96_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX5608031.1"
                     /translation="MRMVRKHGTPFLLLVVGLALGFFLSAHWVPNVAAVGRQDYESLE
                     AFSNILSIVKKNYVEDVETKSLVNGAINGLLSSLDPHSAYLTPDLYKELQSDTQGRFG
                     GLGIEITVKGGILTVVSPIEDTPAAKAGIKPGDQIFKIEEEFTKDMSLVDAVKKMRGP
                     RGSKINLTIKREGVSDLIDVSLVRDIIRVQSVRSRTLEPGYDYIRLAQFQERSDRDMH
                     RALEKMAAEKDGIKGLVLDLRNNPGGLLTQAVRVSDMFLESGLIVYTEGRIEAQKQKY
                     FAQKEGTWNDFPIVVLVNGGSASASEIVAGALQDHRRAVVLGTKTFGKGSVQTILPLD
                     DNSALRLTTARYFTPKGRSIQATGIVPDITVENTTGQEKPDAPRRPSLREENLPGHLQ
                     NPQQSPGIPQEQIEKEKQIPSSGPTGDETIDNDAQLKRALDLLKSWDVFKQIVQKKAA
                     "
BASE COUNT          842 a         1084 c          917 g          826 t
ORIGIN      
        1 ccataatatc ccctaatatc ccctgcccag aacacaaagt aagataagat taagagaaaa
       61 ttttcagcaa ttcgagaggg ttgatacggg ccccggagag acgcgcgccc caatgaagat
      121 gcggcccagt cactcgcccg gaagctccgg acaatccgat aatatcgccc cgtttcaccg
      181 acgatccttg cgcgactttc aattctgaca aatgaaaata cattgtgaag agtccgccgc
      241 cgtgatccag aacaacgctt cctccggcaa agaaataatt gccaatcagc acgacccggc
      301 cgtaattact cgcgacgacc tcggtcccga atggcgcaga taggtccgtg ccgctatgcg
      361 gcgcccgtgg cgagccattg atgatgcggc gcatgccgaa ggatgatgca gaggcctctt
      421 gaggcaccgg ccgaacaaac ggcgcttccc agagccgctc gggcgacgaa aatgcaaacg
      481 cacgagcgaa atccgcctgc tcacgactga tctcctcagc ggtttcgggg ctcacctgat
      541 cgaagctcga cgggacgctg aatgcctccg tcctgaaggc cttcgttttg acatgcaccg
      601 gcaccttgcg ttcataaata gtgcgccgag tggtcgccag caacaggagc ttggaaatcc
      661 cgggtttcgc ttcgatatcc gcgccaacga gaccggtgaa cgaggagcca ccgctttgct
      721 gaaaatagac ctgcgtttgt cccacacggc cttccactgc tgccaatgtt gcgccgctga
      781 tgcgaacttc agccaatccc ccttggagaa tttccaaggc attcggccaa tgcacatcgg
      841 tgcgcccagc agcgtgcaca agcagggtct gctcaagaca gtagctgagc agcgctccaa
      901 ttatccagcg cgggcaccta ttcatttttc tcgacacgac aaaccgattt tccttgcgag
      961 aaagtaatcc gaagacgatc gcccaccgtc aaggcatctg ctcgcttaac gatctgctct
     1021 tcgggcagct tgtgtgcgat cgcatagcca cgctcgagca ccgccagcgg actcaacgca
     1081 tttaaccgtg ccgcgcattc agacaaatcg tttttgtttt tccgtagccg ctcttgaaag
     1141 cgatgtaata ggtcaatcga tagatcatcc agccgctgtt gatggtctcg cagcttacgg
     1201 ctcggatcag ccaagcgctt cagtaacccg cgaagagttt cccgcaccga cgcgagggcc
     1261 agatcgacgg cccggcgaag ttgcagctca agaagatcaa cttgctcgcg caaatcgagt
     1321 ttacgcggaa cgatcatttc tgcagccgcg gtgggcgtcg gagcacgaca atctgcgaca
     1381 aaatccgcga tcgtgaaatc gatctcatgc cccaccgccg aaaccactgg aacattcgaa
     1441 gcgaaaatcg cccgcgccac cacttcttca ttgaaagccc ataaatcctc cagcgatccg
     1501 ccgccgcgac cgacgataat cacgtcgaca gtgcccaggc gatttaaatc gtggataccg
     1561 cgtgcgattt cccttccggc gccgtcaccc tggaccttta ccggccgaat aataatccgc
     1621 cgatcgggga agcgatcatc gataatgcgc aacatgtcac gtaaagcggc gccgccgtcc
     1681 gacgtgacaa taccgatcgt ttttggcagg aacggcaacg gtcgcttgcg ctcctcggca
     1741 aagagaccgt ccgcaagcag cttttttttc agttgctcga aggctatgta gagcgcgcct
     1801 tgtccctgcg gttcgatttc ctcaacgtac agttgcagat cgccacgaac cgtataaacg
     1861 ctcaccctac cgaaacataa aaccgccatg ccattttcag gagtgaagcg caggcttaag
     1921 ccctggcggc gaaacatgac cgcgcagatt tggctacgat cgtctttcag tgtgaaataa
     1981 cagtggcctg agggcggaac ccgaaagttg gaaatctcgc cttgtaccca gactgtgtcc
     2041 aagtgcgatt caagcgagcc tttaatcagc tcgttgagct cgctcacact caaaaatcgt
     2101 cgtatggccg ctgcaaacaa cggcagatcg gcctcgtcgt tcagcacccg taccatttag
     2161 cttctagctt ttagcacaaa aaaattagcc ctccgagggg agggctaatt gagcataatc
     2221 atgccgtcag gttttcggca ggtttaagcc gccttttttt gtacgatctg tttgaacaca
     2281 tcccagcttt ttagcaggtc gagggcgcgc ttgagttgcg cgtcgttatc gatggtttcg
     2341 tcacccgtgg gtccagacga cggaatctgc ttttccttct cgatctgttc ttgcggaatg
     2401 ccgggcgatt gctgcgggtt ttgcaggtga cccggcaaat tttcttctcg taagcttggc
     2461 cggcgtggcg cgtccggctt ctcttgcccg gtggtattct caaccgtgat atcgggtacg
     2521 ataccggtcg cctgtatgga acggcccttg ggtgtgaaat aacgcgcggt ggtcaaacgc
     2581 agcgccgagt tgtcatccaa cggcagaatg gtttgcacgg atcccttgcc gaatgttttg
     2641 gtgcccagca ccacggcgcg ccgatgatcc tgcagcgcgc ccgcgacgat ttccgaggcg
     2701 ctcgcgctac cgccatttac cagaaccacg atcgggaaat cgttccaagt cccttccttt
     2761 tgcgcaaagt atttctgctt ttgcgcttca atacgcccct cggtgtaaac aatcagaccg
     2821 gactccaaga acatatcgga gacgcgcacg gcctgggtca aaagaccgcc gggattattg
     2881 cgcaggtcaa gcacgagacc tttgatgccg tccttttctg cggccatctt ttccagcgcg
     2941 cggtgcatat cccggtcgct gcgttcctga aactgtgcca acctgatgta atcgtatccc
     3001 ggctctaatg tgcggctgcg cacgctctgt actcggatga tgtcccgcac cagacttacg
     3061 tcgatgaggt ctgaaacccc ttcacgcttg atcgtcaaat tgattttact acctcttggg
     3121 ccgcgcattt tcttgacggc atccacgagc gacatatctt tggtgaattc ttcttcaatc
     3181 ttgaagattt gatctcccgg cttgatgccc gccttcgccg caggcgtatc ttctatcggc
     3241 gaaacaacag tcaagattcc tcccttgacc gtgatttcaa tgccgagacc accgaaacgg
     3301 ccttgcgtat cactttgcag ctccttgtaa agatcgggtg tcaaataggc gctatgcgga
     3361 tcgagcgaac tcaacaggcc attgatcgcg ccgttgacta aacttttcgt ttccacgtcc
     3421 tcgacgtaat tcttctttac gatggaaaga atgttgctga aggcctccag gctttcataa
     3481 tcttgccgcc ctacggcggc gacattcggc acccagtgag ccgataagaa aaatcccaaa
     3541 gccagaccca cgaccagaag caaaaacggc gttccatgtt tacgaaccat tcgcatcgcg
     3601 ctctcctatc aaacgtgacg gctcagttta tcttaagaga aataaaatag ctattgctta
     3661 cgaaaccat
//
LOCUS       DASULZ010000781         6914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_699894, whole genome shotgun sequence.
ACCESSION   DASULZ010000781 DASULZ010000000
VERSION     DASULZ010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6914
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699894"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VFY96_16015"
     CDS             complement(<1..731)
                     /locus_tag="VFY96_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608032.1"
                     /translation="MTNRRDFTAESREYRENCSLCGLGASAVLESWFVAQKGRKGTHI
                     EKTGEAYENMTTKPSSEPNRTVGRDTIRSRLVLEVASELVDQTNWKERQVRVSLRQQG
                     ADEWPITFFASDGPAAIDQVARGEVQLALINPAGFLALAARGTGPFAAPLPLRAITVI
                     PSPDQLAFAVTQRAGLNSFRAVREQRYPLRISLRGQRDHALHPVVKEVFAAAGFSLDD
                     LRAWGGQVRYDDGLPQNENRLGAVAR"
     gene            complement(724..1818)
                     /locus_tag="VFY96_16020"
     CDS             complement(724..1818)
                     /locus_tag="VFY96_16020"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5608033.1"
                     /translation="MTMKGKAAIFVAPKQPLVIDEVEFPDPGPDQVLVKLFASGVCHS
                     QLHTIQRPPRPGHRLPELLGHESTGVVVAKGREVHHVKEGDHVITTWVDRDNATTNQP
                     LVMHALNDRPQYAADWRGKEVMHSAATWAECALAQERVVLPLPVGIATDVTSILGCAV
                     MTGAGAIINTLQVRSGQSVVIIGSGGIGLCAISAAAVVDSYPIIAVDVSDQKLEFARR
                     FGATDVINSKKIDPIQRIKDLTDGGADFAIDAVGLPETQEQILRAVRPGYSGRSRGGT
                     ALLIGITPPDAKAILDTSLFIGSRSFTRTSGGDCKPERDFPTFVRWYREGKLKLDELV
                     TSRYKLEQINEAVDDLEHGRILGRGILTFD"
     gene            complement(1982..3109)
                     /locus_tag="VFY96_16025"
     CDS             complement(1982..3109)
                     /locus_tag="VFY96_16025"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX5608034.1"
                     /translation="MAKLNNPTPADVERYRQNYMVEMDGIALYRSMAAVERDSQRAAI
                     FEKLAQNEERHAQRWAKLIQSGGGSVPAHKPSARVRLLGLLARQFGTRRVVPIISNME
                     ARDEAGYMRQPEAAGLPAEERAHSRTLLAMEGKTSGQDSIAGAERWHTTATGGALRAA
                     VFGINDGLLSNFSLVMGFAGAEAKPEYIILAGVAGLLAGSFSMAAGEYVSMRAQREVF
                     EQQIAIEKEELEMSPREEEEELSLIYQAKGIPEHEASRLANRIIQNPKTAIDTLAREE
                     LGLDPAGLGSPWTAATSSFIAFVLGAFVPVAPYLLSSGTRAWVASATLSFCALFSVGA
                     VLSIFTSRGPLRSGARMLGIGLLVSAITYTVGWLLGVSVAG"
     gene            3374..4141
                     /gene="gloB"
                     /locus_tag="VFY96_16030"
     CDS             3374..4141
                     /gene="gloB"
                     /locus_tag="VFY96_16030"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011431823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEX5608035.1"
                     /translation="MKIVQIPLLRDNYAYLLICDKTKEAAIVDPSEAAPVLRRIEAEQ
                     VAFRAILNTHHHRDHTGGNEGILAQHKVEVYGHKSDQSRIPGLTHGLDEGDEVKIGDL
                     KGTIFFIPGHTTGHIAFLFDNNLFCGDTLFTAGCGRLFEGTPEQMHASLKKLMALPDN
                     TKVYCGHEYTESNLRFAISVEPRNPKLVSRFERVQGWRSRGTPTVPSTMEEEKQTNPF
                     LRWDSKELQASVKSVNPNTGLDPVSVFAAVRKMKDSF"
     gene            4304..4777
                     /locus_tag="VFY96_16035"
     CDS             4304..4777
                     /locus_tag="VFY96_16035"
                     /inference="COORDINATES: protein motif:HMM:NF016089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TQO small subunit DoxD"
                     /protein_id="HEX5608036.1"
                     /translation="MNLKERTYLWYLAVLRIYIGFYILSQGLRKFQRNFPKGDWIGRQ
                     IGDLHTVDLYPWYKKFLVNYVAPHHELVGQLVMIGEIAVGGCLLVGFLTRASAAIGLF
                     MLINYYLGVGMARGGATLAQQQTFIVTLAIFVLASPGRTLGFDGLVFGKTKGKAA"
     gene            4774..5883
                     /locus_tag="VFY96_16040"
     CDS             4774..5883
                     /locus_tag="VFY96_16040"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5608037.1"
                     /translation="MRRSNFATPEGTERYRKRFAATVAPEHFRSLQRLWVSSIGLGTY
                     LGNHDETTDAQYHQAVVAAIESGCNVIDTAINYRCQRSERSIGTALKELARKGYGRDE
                     LVIATKGGFLPFDGMPPTDPKNYVIKTFIESGVATGADIVGGYHCMTPRYLSNQLDAS
                     LSNLDLECVDIYYVHNPESQLGKVSPAAFDERLIKAFEALEEAVARGKLRVYGTATWN
                     AYRNPPEAKDYLSLAHVVSLAEQAGGKDHHFRVVQLPLNLGMTEALSNANQQVDGKSV
                     TLLEAAQHFGVTVMCSASVLQGQLTRNLPDLIRDTFGGLETDGQRALQFVRSTPGVTT
                     ALVGMKQLNHVEENLKTARIPPAPWEQYSKLFQTA"
     gene            complement(6068..>6914)
                     /locus_tag="VFY96_16045"
     CDS             complement(6068..>6914)
                     /locus_tag="VFY96_16045"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5608038.1"
                     /translation="SANRAVALVKQGLDLGINFLDTAQTYGTEPIVGRAIAGVRRDQL
                     VISTKKTLPSEDDPDPETEVTKGLEQSLKLLGTDYVDVYHLHGLEPKEYGFAKERLLP
                     LMRRLKEQGKIRFIGVTEGFVPDPSHQMLQSSLAENVWDVVMVGFSMLNPSARKTILP
                     TTMDTGVGVLNMFAVRRALSQPQRLQALCAELIASKAIDADGLDPSDPLGFVFQMSDA
                     ASLPEAAYRFCRHQPGVHVVLTGTGNPEHLRENVAAILKPALPAPVLRKLDHLFGPLD
                     SLTGN"
BASE COUNT         1508 a         2015 c         1902 g         1489 t
ORIGIN      
        1 cgcgctaccg caccgagacg gttttcgttt tgcggcaagc cgtcatcgta gcgcacctgc
       61 ccgccccatg cgcgcagatc gtcgagggaa aaaccggctg ccgcgaagac ttccttgact
      121 acgggatgaa gggcatgatc cctttgaccg cgcagggaaa tccgtagcgg atagcgttgc
      181 tcgcgaaccg cgcgaaacga gttcaagccg gcgcgctgag tcacggcaaa cgcaagctga
      241 tccggcgatg gaattacggt aatggcgcgc aacgggagcg gcgcagcgaa cggtccggta
      301 ccgcgcgcgg cgagagccaa aaaccccgcc ggattgatga gcgccaactg cacttcaccg
      361 cgcgcgacct gatcgattgc cgcaggcccg tcactggcga aaaacgtgat cggccattca
      421 tcggcgcctt gctgccgcaa gctgacgcgg acctggcgct ctttccagtt ggtttgatcg
      481 acgagctccg acgcgacttc gagaaccaag cgcgaccgaa tggtgtctct gccgaccgtc
      541 cggttgggtt cactggaagg ttttgtcgtc atgttttcat atgcctcgcc ggttttttcg
      601 atgtgcgtgc ctttgcgtcc tttctgcgca acaaaccatg actcgagcac cgcggaggcg
      661 ccgaggccgc agagagaaca attctctctg tattctctgc tctccgcggt gaaatctcgc
      721 cggttagtca aacgtcaaga ttccgcggcc aagaatacgt ccgtgctcta agtcatcgac
      781 cgcttcgttg atctgctcaa gtttgtagcg gctcgtgacg agttcatcga gtttcaactt
      841 gccctcgcga taccagcgaa caaaggtcgg gaagtcgcgt tccggcttgc agtctccgcc
      901 gctcgtgcgc gtgaacgacc ggctgccgat aaacagactg gtgtcaagga tcgcttttgc
      961 gtccggcggt gtaatgccaa taagcaatgc cgtgccgcct ctgctcctcc cggagtaacc
     1021 gggcctgacc gcgcggagaa tttgctcctg agtctccggc aatccgacag catcgatggc
     1081 gaagtcggcg cctccgtcag tcagatcttt gatcctttga atgggatcga tttttttcga
     1141 attgatgaca tcggttgcgc caaaccgtcg agcaaactcg agcttttgat cgctcacatc
     1201 gacagcaatg atcgggtagg aatcgacaac ggccgctgcc gagatcgcgc agaggccgat
     1261 tccgcctgag ccgatgatca ccaccgattg cccgctccgc acctggagcg tattgatgat
     1321 cgcgccggcg ccggtcatca ccgcgcaacc aaggatcgaa gtcacgtccg tcgcgattcc
     1381 cacaggcaat ggcaaaacga cgcgctcctg tgccaaggca cattctgccc atgtcgctgc
     1441 cgaatgcatg acctctttgc cgcgccagtc cgccgcgtat tgcggacgat cattcagtgc
     1501 gtgcatgacc agcggctgat tagtcgtggc gttatcgcga tcgacccacg ttgtgatcac
     1561 gtgatcgcct tctttcacgt gatgaacctc gcgtcccttt gccacgacga ctccggtcga
     1621 ttcatgtccc aaaagctccg gcaaccgatg tcccggccgc ggcgggcgct ggatcgtgtg
     1681 cagctgcgaa tgacatacgc cgctggcaaa taacttcacc agaacttggt ccggccccgg
     1741 atccggaaac tcaacttcgt cgatgaccaa cggttgcttc ggtgcaacaa atattgccgc
     1801 cttgcctttc atggtcatgg ttcaccgtgt tgcatttggc tcgcgaatgg ctcgaggtgc
     1861 tggccggagc gccgagattc tagccacatg cgtgactgca gtgcgagcgt aatgtcctgt
     1921 ttaaacatcc acttgccgcg ctgcacgttt cagagaatag cgtcatcatc agagcgtcgg
     1981 tctatccagc gaccgagaca cccaggagcc aaccgacagt ataggtgatc gcggaaacca
     2041 ataagccgat gccgagcata cgcgcgccgc tccgtagcgg accgcgagac gtaaaaatcg
     2101 aaagcacagc tccgacactg aataacgcac aaaaactcag agttgcactg gcaacccacg
     2161 ctctggtccc ggacgacaaa agatacggcg caacgggaac gaaggcgcca agcacgaaag
     2221 cgatgaacga actcgtcgct gccgtccagg gagagccgag cccggctgga tccaaaccga
     2281 gttcttcgcg tgccaacgta tcgatcgcgg ttttcggatt ctgaatgatt cggtttgcga
     2341 ggcgggaggc ttcgtgctcg ggaattcctt tggcctgata aatcaaagac agctcttcct
     2401 cttcctcccg gggcgacatt tccagctctt ctttttcaat ggcgatttgc tgttcgaaga
     2461 cttcgcgttg ggcgcgcatc gaaacgtatt ctcccgctgc catggaaaaa gaaccagcta
     2521 gcaaaccagc aacccccgcg aggatgatat attccggctt ggcctccgct ccggcgaaac
     2581 ccatcacgag actgaagttc gagagcaagc catcgttgat gccaaagacg gccgcgcgca
     2641 aagcgccgcc agtggcggta gtatgccaac gctcggcgcc cgcgatggaa tcctggcccg
     2701 aggtttttcc ttccatcgcg agcagagttc ggctgtgtgc gcgttcctcc gctgggaggc
     2761 ccgccgcttc gggctggcgc atatagccgg cttcgtcgcg cgcctccata ttactgataa
     2821 tcggcaccac cctgcgcgtg ccgaactgtc gcgccaacaa accgagcaaa cgcacccgtg
     2881 ctgatggttt gtgcgccggc actgagccgc cgccggattg aatcagcttg gcccagcgct
     2941 gcgcgtggcg ttcttcgttc tgtgcaagct tttcgaagat tgctgcccgt tgactgtcgc
     3001 gctcgaccgc cgccatggag cggtataagg ctataccgtc catctccacc atatagtttt
     3061 gccggtaccg ctccacgtcc gccggcgttg gattgtttag tttcgccatc ccgattattt
     3121 tgttgtactt gcaacgagca gtctcatcct tgccacagcc aaagcaaaag agtcaaattg
     3181 ccgcaagcgc aacagcagta ccgaaaatta gcgcgatgca aacgagtcct gcttttattt
     3241 gctcactcta cgctgagtca cgattaaagt ggatattcga gaaaatttag gacaatataa
     3301 gaccattcct ggtacaagac tcgagcggcg cctcaaatcc aacattttct aaggaatctg
     3361 actgagcaat agtatgaaaa tcgttcaaat ccctctgctg cgcgataact acgcctatct
     3421 tctgatctgc gacaaaacca aagaagcggc tatcgtggat ccatcggaag ccgcaccggt
     3481 gctgcgccgg atcgaggccg agcaggtcgc gtttcgggcg attctcaaca cccatcatca
     3541 tcgcgatcac accggtggaa acgaaggtat tctggcccag cacaaagtcg aggtgtacgg
     3601 ccataaaagc gaccagagcc gcattccggg attgactcac ggcctggacg aaggcgacga
     3661 ggtaaaaatt ggcgatctga agggaacgat ttttttcatt ccggggcaca cgaccggtca
     3721 catcgctttt ttgttcgaca ataatctctt ttgtggcgac actctgttca ccgcgggctg
     3781 cggccgactc tttgaaggca caccagaaca aatgcacgcc tcgctcaaaa aactgatggc
     3841 gctcccggat aatacgaaag tctattgcgg gcacgaatac actgagagca acttgcgatt
     3901 tgcgatctcc gtggagccta gaaaccccaa gctggtttcg cgctttgagc gcgtccaggg
     3961 atggcgctca cgtggcacac cgacggtgcc atcgacgatg gaggaagaga agcaaacaaa
     4021 tccttttctt cgctgggata gcaaggagct tcaagcgagc gtcaaatcgg tcaaccccaa
     4081 caccggcctt gaccccgttt cggttttcgc cgccgtcaga aagatgaagg attcctttta
     4141 aggcctcggc caaacggcag gagctgtgtt ttccattgaa tctattcctt gatcatcgct
     4201 cgcatcaggg atatggtaga gtcgttttca cagtcgcctt caatccgccg cgcgccgcat
     4261 agacgcgccg ctcacgcgct tccttcactg tgcggagagc atcatgaacc tcaaggaacg
     4321 gacttatctc tggtatcttg cggtgctgcg catctatatc ggcttctaca tactctccca
     4381 aggattgcga aaatttcagc gtaattttcc caagggtgac tggatcggcc gacagatcgg
     4441 agatctccac actgtcgatc tttatccctg gtacaaaaaa tttcttgtca attacgtagc
     4501 gccgcatcac gaactagtcg ggcaacttgt gatgatcggt gagatcgcgg tcggcggttg
     4561 cctgctggtc ggatttttaa cccgggccag tgcggcaatc ggactattca tgttaatcaa
     4621 ctactatctc ggggtcggga tggcgcgagg cggcgcgact ttggcgcaac aacagacttt
     4681 tattgttacg ttggccatct tcgtgcttgc cagcccgggt cgcactctgg gattcgatgg
     4741 gctggttttc ggtaaaacaa aagggaaagc cgcatgagac gatcgaactt cgctacgccc
     4801 gaaggcacgg agcgttaccg aaagcgtttt gcagccaccg ttgctccgga gcacttccgc
     4861 agtttgcagc ggctatgggt ttcttccatc gggctcggca cgtatttggg caatcatgac
     4921 gaaaccaccg acgcccaata ccaccaagcc gtcgtcgccg cgatcgaatc cggctgcaat
     4981 gtcatcgata ccgcgatcaa ttatcgttgt cagaggagtg aacggtcgat tggcacggca
     5041 cttaaggaac tcgcgcgaaa aggctacggg cgggacgaac ttgtgattgc aaccaaggga
     5101 ggttttttac cgtttgacgg tatgccgccg accgatccga aaaattacgt cataaagacc
     5161 ttcatcgaga gcggcgtggc gaccggcgcg gatatcgtcg gcggctatca ctgcatgacg
     5221 ccgagatatc tgtcgaatca actcgatgcg agcttgagca atctcgatct tgagtgcgtc
     5281 gacatctact acgttcacaa tcccgaaagt cagctgggca aagtttcacc cgcagcattc
     5341 gacgaacgct tgataaaagc gtttgaggcg ctggaggaag cggtcgcgcg aggcaagctc
     5401 cgggtttacg gcacggccac ctggaacgcc tatcgcaatc cgcccgaagc caaggactat
     5461 ctctcgttag cgcacgtcgt cagcttggcg gaacaggccg gcggcaaaga tcaccacttc
     5521 cgcgtcgtcc agttgccgct gaaccttggc atgactgaag ctctctccaa tgccaatcaa
     5581 caggtggatg gaaaatcggt gaccctgctc gaagcagcgc aacatttcgg cgtcacggtc
     5641 atgtgcagcg catccgtact gcagggccag ctgacgcgaa atctcccgga tctaattcgc
     5701 gatacttttg gaggcctcga aaccgacggc cagcgcgcgc tgcagtttgt gcgctccacc
     5761 cccggggtga ccacggcgct agtgggaatg aaacagttga accacgtcga agaaaatctt
     5821 aaaacggcgc gtattccgcc tgcgccctgg gagcagtact ccaagctctt tcaaaccgcg
     5881 tagctacgaa cagcctgcct cggtatgcgc ttcgcgctac tcgagaactt ggaatggatc
     5941 ccatacgttc tgctcatccg cagctgtttt ggaagatcgc tcccgttggt gaccaccgat
     6001 tctccgagta gcgaccctgg gtagtcgcgt atcgaggcgc cgttttccat cctcgatttt
     6061 caaggtgtta gtttccggtc aggctatcca acggaccaaa taaatgatcg agctttcgga
     6121 gcacgggtgc cggcaaagct ggttttaaaa tcgcagcgac attttctcgc aaatgctccg
     6181 ggttgccggt tccggtcaag acgacgtgaa cgcccggctg atggcgacaa aaacgatagg
     6241 ccgcctcggg caagctcgcg gcatcgctca tttgaaaaac aaacccgagc ggatccgacg
     6301 gatccaaccc gtctgcatcg atcgccttcg aggcgatcag ttcggcgcac aacgcctgca
     6361 accgctgggg ctggctgagc gcgcgacgaa ccgcgaacat gtttagcacg ccgacgcctg
     6421 tgtccatggt cgtcggcaaa atggttttcc gtgccgatgg attgagcatg ctgaagccca
     6481 ccatgacgac gtcccacaca ttctcagcca ggctcgattg cagcatttga tgcgacggat
     6541 cgggaacgaa tccttcggtc acgccgataa agcgaatctt gccttgctcc ttcaggcggc
     6601 gcatgagcgg gagcaagcgc tctttggcaa aaccgtattc tttcggctcg aggccatgga
     6661 gatgatagac gtcgacgtag tcggtgccga gcaacttgag gctctgttcg agacccttgg
     6721 tcacctcggt ttcgggatcc ggatcatctt ctgatggaag tgtttttttg gtcgagatga
     6781 ccaactggtc acgtcgcacg ccggcgatcg ctctgccgac gatgggctcg gttccgtagg
     6841 tctgtgcggt atcgaggaag ttgatcccaa gatcgaggcc ttgcttcacc agagcgaccg
     6901 cacgatttgc cgat
//
LOCUS       DASULZ010000782         8405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1104049, whole genome shotgun sequence.
ACCESSION   DASULZ010000782 DASULZ010000000
VERSION     DASULZ010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8405
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104049"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1135)
                     /locus_tag="VFY96_16050"
     CDS             complement(128..1135)
                     /locus_tag="VFY96_16050"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5608039.1"
                     /translation="MPIQIDQKSAESLAPQFDEALELVRNEFEGLDAHRQYRNAFARL
                     LKADPLGRVLMMGTDQRDMFAPELRRVIETSVPSNGHIFDFGAGDGQTFALVADSVPQ
                     GTRVSIEEPNPEYLANYRAFLETKPHLRPGLALAAEFDDINAAAERSGEKLPPDGSID
                     LSLALHMIYFIDDLVASVARMVRFLKPDGVLFIVVTDETIGYTGLALKSFIERGGDTG
                     DNARHLSVIDERARLLGLPAEGGGAIAEVLRAAGTPVEIDVRRQPSRLYGHNLADLIA
                     LSSIALLASVEGLEKFEAAAALLRHNPDAVDLRIEDDGARKGMWSVTQPQWVAALRRT
                     R"
     gene            complement(1293..3305)
                     /locus_tag="VFY96_16055"
     CDS             complement(1293..3305)
                     /locus_tag="VFY96_16055"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX5608040.1"
                     /translation="MMMRSRRKLKIAGKKAKKTVFRVEEATIAGVHRAFRAKKLTATE
                     LVNCYLARIKAYNGLCVRGDTDPVTGLQLGDITPIPNAGQLNAFISLNLKEARRIALG
                     FPEKLKRTHTGDDEAKHPDALDRARELDRYFARTGKFAGPLHGIPIAIKDNYDTCDMR
                     TTAAAAAEYADDRPPDDATMVARLRAAGAILLGKTNMDEYAPAGIARSAFGGQTGNPY
                     DTTRIPGGSSGGSAAAVAANLAMCALGTDTSGSVRFPSSCCSLVGLVATQGLVSRDGI
                     VPLSFTRDRGGPICRTVEDTAILLEVLAGYDPKDPLTAACGNRPKISYARHAGGKSLR
                     GRRLGVLRDLMIEASRADRENIRVANEALAAMKKAGATIVDPVNVDKTIADLIPYLEP
                     GWLKRNFPAAFPDSEKPIDRIVSMAFDHRLVPSGARGVNLRMLAAQARGNEAHYAINR
                     YLRERGDAEFKDVNDMLAMPMFSGNLDNLKRAFSPEATTLDSQTETDHLLRMETLRRI
                     ILQVMAENNLEALVYAYTTSPPHTILTNRLAKTVEAYTEPRILKAGTVMSDPSVVPGE
                     PVLQSDLDTYRGSGSSFAVSLSPLTGFPAIVVPAGFTREVYDRVPDESDPNGCRLEGP
                     KQIELPVALEFLARPFEEATLFEIASGFESIIRHRRPPSAFGALVR"
     gene            complement(3493..4302)
                     /locus_tag="VFY96_16060"
     CDS             complement(3493..4302)
                     /locus_tag="VFY96_16060"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5608041.1"
                     /translation="MDSTSQPSSSIFEGYADLPGVRLWYVDTGGTGVPLVLLHANTGN
                     ADGWHYNISGFVEAGYRAIAFDRRGWGRSRANPQTGPQPGTIAEDLHALVEHLKLDRF
                     HLVGVAGGGFAAYDYVLWHPERLRSLVVAASGGAIVDEELSKLREQTTLPEFRSWPPE
                     FREVSTGYMATNPEGLKRWLEIHHHSQQQGAPAQPQRTTITFEKLKTIRVPTLLMPGD
                     QDLQCPPWVMRRQLACIPHAEFIVLPEASHSINWEQPEAFNRHVLEFIREH"
     gene            complement(4484..5470)
                     /locus_tag="VFY96_16065"
     CDS             complement(4484..5470)
                     /locus_tag="VFY96_16065"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5608042.1"
                     /translation="MPIILGICLLLFFGAEAQVFGAGAPPAKIVITPASFSEREGLLI
                     VSQNQGFFRKHKLDIELVLMPNAPIAFSALTAGESHFYYGTTSGTSLGAVLNGLDGVF
                     VAGFINKLIGAFAVTKDIKTPNDLKGKQIGVASLGGGNWMFTMLAFDHWGLDPKRDNI
                     TFRIIGNTGVRAQSIASGIIGGSLLGYTFATNLRREGYPILADLADLNIPFLDSGLYT
                     RKSFVAQHPEVVENVLRALLDGITFIQNPENKSAVVRSLVQWLRLTKTEDAVEGYEFM
                     RKLYTKRIYPTVEGIRNSIRVLALTNEKFRALKAEDLVDDRIVRKLEKEGAF"
     gene            5630..7105
                     /locus_tag="VFY96_16070"
     CDS             5630..7105
                     /locus_tag="VFY96_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX5608043.1"
                     /translation="MAKKSGELLLRDAGGAYALRPILGPLQLTSLGIGAIIGAGIFVI
                     IGVVAKDKAGAATMLSFVVAGTACGFAALCYAEFASMVPIAGSAYTYAYATLGELFAW
                     IIGWDLLLEYGVGAASVAHGWSHYFVDFLGLLQIYLPPTLINSPFDFDPGSGRFVATG
                     AIIDLPALLISAALTIVLVIGIKRSANFNFFMVVIKLAVVLLIIAIGIFYIDPTNWHP
                     FAPYGYAGVSLFGKTVIGQHGAGGEPLGMMAGAAVVFYAYIGFDSVSTHAEEARNPSR
                     DVPIGIIGSLTLCTILYIAVAAVLTGMVPYQQIDIDAPIAKAFSRTGLSWAHALISLG
                     ALTGITSVLMVLMLSQPRIMLAMARDGLLPNAFFGAVHPHFRTPWKSTILTGTVVGLM
                     GALLPLRMLAELVNIGTLLAFVIVCAAVLIMRRTNPEMPRPFRCPLVPVVPLLGIAFC
                     LLLMFSLPVENWLRLIVWLLIGFAIYFSYGRWHSVLGRTLG"
     gene            complement(7128..8186)
                     /locus_tag="VFY96_16075"
     CDS             complement(7128..8186)
                     /locus_tag="VFY96_16075"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5608044.1"
                     /translation="MAKRIILVATICLLINGISGAWAQCQAASAPDLAAYTGADREQR
                     LLAGAKAEGKVVWYTSLAGSSYKELAKGFEAKYPGVKIEPYRGTSSDIMTKISAEAQA
                     KQIIADTIETTLPTLRFFRENKLLIAFTSPHLAKYPPATKESAARGLVYWAIDRETYM
                     GVGYNTNLIAATLVPKNYADLLRPQLKGKIGFVSNETGTRTIGGIVKAKGEEFVRKLK
                     AQEITLHAISGRAMADLVISGEVALSPTIFRDHALEAKAKGAPIDWAAMEIVPTNAGA
                     VALVAQAPHPHAAALLADFLLSPEAAKILGDLDYGSPFKPVSFKLWYPEAGLSTEQYD
                     KAAGKWDKLLREIGRKPL"
     gene            complement(8226..>8405)
                     /locus_tag="VFY96_16080"
     CDS             complement(8226..>8405)
                     /locus_tag="VFY96_16080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608045.1"
                     /translation="LPGKFSHLSVHPGETVTFLTAGGGGYGNPEERDLNAIKRDIDLG
                     YVSEKRAKELYSELQ"
BASE COUNT         1596 a         2504 c         2416 g         1889 t
ORIGIN      
        1 tattcgtgga gatgctcaat ctgcggttgc gcagaaagtc agagccggtt tcgttgcacg
       61 agcgctacgt agtagatgac cgtgtcggcc agtcataaca aacgacggcg gctccgttgc
      121 gagtttgcta gcgtgttcga cgcaaggcag cgacccattg tggttgggtg acgctccaca
      181 tgcctttacg ggcgccgtcg tcttcgatac gcaggtcgac cgcgtcgggg ttgtgacgca
      241 gcagtgcggc ggccgcctcg aacttttcca ggccttcgac gctggcgaga agtgcaatcg
      301 aggagagcgc aatcaggtcc gcgagattgt gtccgtagag ccggctcggc tggcgccgca
      361 cgtcgatttc cacgggagtt cccgcggcac ggagcacttc cgcaatcgcg ccaccgcctt
      421 ccgcgggtag tcccaacaag cgggcgcgct cgtcaatgac tgagagatgc cgcgcgttgt
      481 cgccggtgtc accgccgcgt tcgataaaac ttttcagcgc gaggccggta tagccgatgg
      541 tttcgtcggt cacgacgatg aacagtacac cgtcgggctt caagaaccgc accatcctcg
      601 cgacgctggc cacaaggtcg tcgataaaat agatcatgtg cagcgcgagg ctgaggtcga
      661 tgctgccatc cggcggcagc ttttcccccg agcgctcggc cgctgcattg atgtcgtcaa
      721 attctgcagc gagcgccaga ccgggccgca aatgcggctt tgtttctaga aaagcacgat
      781 aatttgcgag gtattctgga ttgggctcct cgatcgagac gcgggtaccc tgcggtactg
      841 aatcggcaac gagagcaaac gtttgtccgt cgccggctcc gaaatcgaag atgtgaccgt
      901 tactcggcac cgacgtttct atgacccggc ggagctccgg cgcaaacatg tcgcgctggt
      961 cggtgcccat catcagcacc cgcccaagcg ggtccgcttt cagcagccgc gcgaaggcgt
     1021 tgcggtactg gcgatgcgcg tcaagccctt cgaactcgtt gcgcaccagt tcgagcgctt
     1081 cgtcgaattg aggcgcaagg ctctctgcgc ttttctggtc aatctgaatc ggcatgagac
     1141 tgttacgcta acttgacatc cgatcaccag cgttagaccc ttccgcagtt tcccaactga
     1201 tcgcgacgcc gcgagccctg catccaacct gtttgcgcac cgtcgtggtg atctcgaacg
     1261 tcctgtgcat ccgtgtttag agcttcatcg catcatctca caagcgcgcc gaacgcagac
     1321 ggcggacgcc gatggcgtat gatgctttca aaccccgagg cgatctcgaa gagcgttgct
     1381 tcttcgaacg ggcgcgcgag gaactccaga gcaaccggca actcgatttg tttcggacct
     1441 tccaaacggc agccgttcgg atcgctctcg tccggcacgc gatcgtagac ttctcgggta
     1501 aatccggcgg gcacgacgat cgccggaaag ccggtcagcg ggctgagact gaccgcgaag
     1561 ctcgatcccg agcctcggta agtgtcgaga tccgactgta gcaccggctc gcccggcacg
     1621 acgctcggat cggacatcac ggtgccggct tttaaaattc tcggctcggt gtaggcctcc
     1681 acggttttcg ccaagcgatt ggtcaagatc gtgtgcggtg gacttgtcgt gtaagcgtaa
     1741 accagcgcct cgagattatt ttcggccatc acctggagaa tgatccgccg caacgtctcc
     1801 atgcgcagca gatgatcggt ttcggtctgg ctgtccagag tcgtggcctc ggggctgaag
     1861 gctctcttga gattatccaa attgccggaa aacatcggca tggcgagcat atcattcaca
     1921 tccttgaatt cggcatcgcc ccgctcgcgt aggtaacgat tgatcgcgta atgcgcttcg
     1981 ttgccgcgcg cttgcgcggc gagcatgcgc aggttcacgc cgcgggcgcc ggaaggaacc
     2041 agacgatgat cgaacgccat cgagacgata cgatcgatgg gcttctcgga gtcgggaaac
     2101 gccgcgggaa aatttcgctt cagccagccg ggctcgagat aaggaatcag atccgcgatc
     2161 gttttgtcga cgttgaccgg atcgacgatc gtcgcaccgg cctttttcat ggcggcgagc
     2221 gcctcgttcg caacgcggat attctcgcga tcggcgcggc tcgcttcgat catcagatca
     2281 cgcagcacgc cgagccttct cccgcgcagc gattttccgc cggcatgccg cgcatacgaa
     2341 atcttcggcc ggttaccaca cgcggcagtg agcggatctt tgggatcgta gccggcgaga
     2401 acttcgagaa gaatcgccgt gtcttctacc gtgcggcaga tcgggccgcc gcgatccctg
     2461 gtaaatgaca gtggcacgat gccatcgcgg ctcaccagcc cttgggtcgc aaccaggccg
     2521 actaggctgc agcacgacga cggaaaacgc acagagccgg aggtgtccgt acccagagcg
     2581 cacatggcga ggttggccgc taccgcggcc gccgacccgc cgctcgatcc gcccggtatc
     2641 ctggtcgtat cgtaagggtt gccggtctgg ccgccgaagg cgctccgcgc aattcctgcg
     2701 ggcgcgtact cgtccatatt ggttttgccg agcagaatcg ctcccgcggc gcgcagcctc
     2761 gcgaccatag tggcgtcgtc gggcgggcga tcgtcggcgt actccgccgc cgctgccgcc
     2821 gtcgtgcgca tgtcgcaggt atcgtagttg tccttgatcg cgatcggaat cccatggagc
     2881 gggccggcaa acttcccggt gcgtgcgaag taacggtcga gctcccgcgc ccgatcgaga
     2941 gcgtcgggat gttttgcttc atcgtctccg gtgtgggtcc gtttgagttt ttcgggaaaa
     3001 ccgagcgcga tccgtctcgc ttctttcaaa ttcagactga tgaaagcatt gagctgcccg
     3061 gcgttcggga tcggcgtgat gtctcccagc tgcagtccgg tgacggggtc ggtatcaccg
     3121 cgcacgcaga gcccgttata ggccttgatg cgcgcgagat aacaattcac gagctcggtg
     3181 gccgtgagct tcttcgcgcg aaacgcccga tgcaccccgg cgatcgtggc ttcctccacc
     3241 cggaagacgg ttttcttcgc tttcttgccg gcaattttta actttcgcct ggaacgcatc
     3301 atcatccttt acataaaagc gttcaaatgg ttcaagcagt tcaaccgctt tcctccgttt
     3361 aaatcgtcaa agaaatcgac ctccggcaac taacaagact gcctctacca aattacaatc
     3421 atactttgcg agctttgcgc ctccgcctct atccggcaac tgctagtctg ctggctcttc
     3481 tactgcatgt ttctaatgct cgcgaataaa ctctaaaaca tggcggttga acgcttccgg
     3541 ctgctcccag ttgatcgagt gtgaggcttc gggcaggacg atgaactcgg cgtggggaat
     3601 acaggcgagc tggcggcgca tgacccatgg cgggcactga agatcttgat cgccgggcat
     3661 cagcaaagtc ggcacgcgaa tcgttttgag cttttcgaac gtgatggtgg ttctttgcgg
     3721 ctgcgccggc gcgccttgtt gctgcgagtg gtggtgaatc tcaagccagc gtttcaagcc
     3781 ttcagggttg gtcgccatgt agcctgtgct cacttcgcga aactccggcg gccagctcct
     3841 gaactccgga agcgtggttt gctcgcgcag cttgctcaat tcttcatcaa caatggcgcc
     3901 gccgctcgcg gcgaccacca aactgcgcag ccgctcaggg tgccaaagaa cgtaatcgta
     3961 cgcggcgaat cctccgccgg caacaccgac gagatgaaag cggtcgagct tcaggtgttc
     4021 gaccaatgcg tgcaggtctt ccgcaatcgt gccgggctga ggacccgtct gcggattcgc
     4081 tctgcttcga ccccagccgc gccgatcgaa agcgatggcg cgataacccg cttcgacaaa
     4141 acccgagatg ttgtagtgcc agccatcggc attccccgta ttcgcgtgaa gcaatacaag
     4201 cggcacaccg gtaccgccag tatcaacgta ccaaaggcgc acgcccggca gatcggcgta
     4261 cccttcaaat attgagctag aaggctgtga ggtagagtcc atgactttac tccatatggc
     4321 gtttcgcttg actctgtgcg agtccgtcat tcccgcatgc ttttagcggg aatccaggcg
     4381 aatccggact agacccccga ttaatacatt cgggggtgac acggtgtttg caaggtgttt
     4441 tagttcgccc ccgggtgttt tcgaaggaac cgcatacttc accttagaac gcaccctctt
     4501 tttccaattt ccgtacgatg cgatcgtcga ccaaatcctc ggccttgagc gcgcgaaact
     4561 tctcgttcgt caacgcgagc acgcgaatgc tgttgcggat cccctccacc gtcggataaa
     4621 tgcgcttcgt gtagagcttg cgcatgaact catagccttc gacggcgtct tcggtcttcg
     4681 tcaaacggag ccattgcacc aggcttctca cgacggcgct tttgttttcc gggttctgaa
     4741 taaacgtaat cccgtcgagt aaggcgcgca gcacgttttc gacgacctcc gggtgttgtg
     4801 cgacgaaact cttgcgcgtg taaagtcccg aatccaaaaa aggaatgttg agatcggcga
     4861 gatcggccaa aatggggtag ccctcgcgcc gcaaattggt cgcgaaagta tagcccaaca
     4921 gcgagccgcc gatgattccc gatgcgatcg actgcgcgcg gacgccggtg ttgccgatga
     4981 tccgaaaggt aatgttgtcg cgcttgggat cgaggcccca gtggtcgaac gcgagcatcg
     5041 taaacatcca gttgccgccg cccaggctgg ccacgccgat ctgttttcct ttgagatcgt
     5101 tcggcgtctt gatgtctttc gtcaccgcaa atgctccgat cagtttattg atgaatcccg
     5161 cgacgaagac accgtctaat ccattcagca cagcgccgag gctcgtgccc gacgtggtgc
     5221 catagtagaa atgcgactcc cccgcggtga gagcggaaaa agcgatcggg gcgttcggca
     5281 tcaacaccag ttcgatgtct aatttgtgct tgcggaagaa gccctgattt tgcgatacga
     5341 tcagcagccc ttcccgctcg ctgaaactcg ccggagtgat gacgattttt gccggtggcg
     5401 caccggcgcc aaacacctga gcttccgcgc caaaaaagag caacagacaa attcccagga
     5461 tgattggcat gccgaccttg acctcaagag aagcgtgacc ggatactaga aggaagctcc
     5521 gatagcgtca agcaagaaat atcggtctgc ggctgcacac gactgtcatt gcattcaacg
     5581 cgagcgcatt gtatttggct cacctatgac accgttgcgg cgctcgttga tggcaaaaaa
     5641 atccggtgag cttttattaa gagacgccgg cggcgcttac gcgctgcggc cgattctcgg
     5701 gccgctccaa ctcaccagcc ttgggattgg cgcaatcatc ggcgccggca tttttgtcat
     5761 tatcggcgtc gttgccaaag acaaagccgg cgcagcgacg atgttatcgt tcgtggtcgc
     5821 tgggacggcc tgtggttttg ccgcgctatg ttatgcggaa ttcgcttcca tggttccgat
     5881 cgctggatcc gcttacacct acgcctatgc caccttgggc gagcttttcg cgtggatcat
     5941 cggctgggat ctgctgctcg aatacggcgt cggggcggcg agtgtcgccc atggctggtc
     6001 gcactacttc gttgattttc tcggtctgct gcaaatttat ctgccgccca ctctcattaa
     6061 ttcccctttt gatttcgatc cggggagcgg ccgtttcgtt gcgaccggcg cgatcatcga
     6121 tttgccggcg ctgttgatat cggcagcgct cacgatcgtt ctcgtgatcg gtatcaagcg
     6181 cagcgccaac ttcaattttt tcatggtggt gatcaagttg gcggtggtgc tcctgatcat
     6241 tgccatcggg attttctaca tcgaccccac caattggcac cccttcgctc cgtacggcta
     6301 tgccggcgtc agtcttttcg gcaagaccgt aatcggacaa catggagcgg gtggcgagcc
     6361 cctgggaatg atggcgggcg ccgcggtcgt gttttatgcg tacatcggct ttgactcggt
     6421 ctccacccat gccgaagaag cgcgcaatcc cagccgcgat gtgccgatcg ggatcatcgg
     6481 ttcgctcacg ttgtgcacca tcctatacat tgcggtcgcg gcggttctga ccggcatggt
     6541 tccctaccag cagatcgata tcgacgcgcc gatcgccaag gctttcagcc gaaccgggct
     6601 gtcctgggcc catgcattga tttcgcttgg cgcgctcacc ggcatcacct ctgtgttgat
     6661 ggtgctgatg ctgagtcagc cgcgcatcat gctggcgatg gcgcgtgacg gtcttttgcc
     6721 caacgctttt ttcggcgccg tgcatcccca cttccgcacg ccatggaaat cgacgatttt
     6781 gaccggcacc gtagtaggac tgatgggcgc attactgccg ctccgcatgc tcgccgagct
     6841 cgtgaacatc gggacgctcc tcgcattcgt catcgtctgc gccgcggtat tgatcatgcg
     6901 caggacgaat ccggagatgc cgcgcccttt tcgctgcccg ctcgttcccg tcgtgccgct
     6961 gctcggcatc gccttctgct tgttgttgat gttttctttg ccggtggaaa actggctgag
     7021 attaatcgtc tggctgctga tcggctttgc gatttatttc agctatggaa gatggcatag
     7081 cgtgctcggg cgcaccctcg gatagatctt tcccccggtc aactgcatca aagcggcttt
     7141 cggccgattt cccgcagtag cttgtcccac ttcccggcag ccttgtcata ctgttccgtc
     7201 gagagtccgg cctcgggata ccacagcttg aagctcaccg gcttgaatgg gctcccgtag
     7261 tcgaggtcgc cgagaatttt tgccgcttcg ggactcagaa gaaaatcggc cagcagcgcc
     7321 gccgcgtgtg gatgcggcgc ttgcgcaacc agcgccacag cgcctgcatt ggtgggaaca
     7381 atttccatcg ccgcccaatc gattggagcg cctttggctt ttgcttcgag cgcgtgatcg
     7441 cggaagatcg tcggcgacag cgccacttcg cctgagatca cgagatcggc catcgcgcgg
     7501 cccgaaatcg catgcaacgt gatctcttgc gctttcaact tccgtacgaa ctcctcgcct
     7561 ttcgctttga caatgccgcc gatggtgcgc gttccggttt cgttcgaaac aaagccgatc
     7621 tttcctttga gctggggcct gagcagatcg gcataatttt tcggcaccag agtggcggcg
     7681 atgagattgg tattgtagcc aacgcccatg taagtttcgc ggtcaatggc ccaatagacg
     7741 agcccgcgcg cagcactttc cttcgtggcc ggcggatact tggcgagatg gggcgaggtg
     7801 aaagcgatta gaagcttgtt ctcgcggaaa aagcgcaacg tcggcagcgt cgtttcaatc
     7861 gtatcggcga tgatctgctt cgcctgagcc tcggcgctga ttttcgtcat aatgtccgag
     7921 ctggtgccgc gatagggttc gatcttgacg ccgggatatt tcgcttcgaa gcctttggcc
     7981 agctctttgt aagagctccc ggcaagcgat gtgtaccaaa ctactttgcc ctctgctttc
     8041 gctccagcca ggagccgctg ctcccgatcc gccccagtgt aagccgcgag atccggagct
     8101 gaagccgctt gacactgcgc ccatgcgccg ctgataccgt taatgaggag acagattgtc
     8161 gcaacaagta tgattcgttt cgccatgcgc ttcacgagtg ctcctttcaa atgctgcgcg
     8221 cttccttact gcagctccga gtagagctcc ttggcccttt tctcggagac gtaacctaaa
     8281 tcgatatcgc gtttgatcgc gttgagatca cgttcctccg ggttgccgta tccaccgccg
     8341 ccggccgtaa gaaaagtcac cgtctctccg ggatgaaccg ataaatggct gaactttccg
     8401 ggcag
//
LOCUS       DASULZ010000783         2771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_598939, whole genome shotgun sequence.
ACCESSION   DASULZ010000783 DASULZ010000000
VERSION     DASULZ010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598939"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(289..1713)
                     /locus_tag="VFY96_16085"
     CDS             complement(289..1713)
                     /locus_tag="VFY96_16085"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HEX5608046.1"
                     /translation="MGEEVLENLAADINKLLSEPIGSRRVEITDLTPRDGQQCKLATR
                     VTTDDLLPLCASLDACGFHAVEVWGGATFDVCMRYLKEDPWERLRRIKSVMPKTKLQM
                     LLRGQHILAYRPYTDTIVYKFVERAIANGMNVFRIFEATNDFRNIEVATRAVKEMGGE
                     AHVEVNYTVSPVHTLEKWMEYAEQLIEIGADWLSFKDATGILTPFDSYRIIKGIKDRA
                     KDKLPVLLHCHDMGGTSSMSHLMAILAGVDMIDTVLSPLSFGSAHPATETMVASLKGT
                     PFDTGIDVKILEEPARITRQIKEKYEKYETGYTGVSADVFIHKVPGGMISNMVAQLKE
                     AGRMDLMEAALKETPSVEKDLGHPPLLTPTSQIVGVQAVLNVLAGERYKIVSRETSDY
                     VKGKYGQPPGSVNKDLVRKIMKDKEPDYSRRSGEMADPSDWEKSIKDLGPLAKTDEDI
                     LLGVLFPMQAKEFLTLREKGELPN"
     gene            1926..2273
                     /locus_tag="VFY96_16090"
     CDS             1926..2273
                     /locus_tag="VFY96_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608047.1"
                     /translation="MKIVRLYTGQDNESHFEDIEVELNPTGRMEASELQPTQGIIFRS
                     APPDHLSNYHPVPKRQYVITLSGQVEIETGDGTIRRFGPGDVMLAEDTTGHGHITRVV
                     GGAPRDYVFIPLK"
     gene            2366..>2771
                     /locus_tag="VFY96_16095"
     CDS             2366..>2771
                     /locus_tag="VFY96_16095"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5608048.1"
                     /translation="MMVATLGIALPEIRESFSLSELAAGSLFSVMMIVAALTSAGAGR
                     LADRIGPKKVLIAGLTLLGVGFAGAGMSEHSIVFASFLAVTGIGYGFTPPSLYLIMSE
                     LLPQRRGLGASLISVAYGIGGAIGALISSRVAA"
BASE COUNT          590 a          796 c          690 g          695 t
ORIGIN      
        1 actacttagc cgttcatcct gagcgtgtcg aaggacgaac ggcgagtttt cacaaagtct
       61 gccgaaggga gaaatccttc caagccgtca tcgccccgtc gaaggctcga agactgcaaa
      121 agcagaaaga tttctcgcta cgctcgaatg acaagataat tttctacgcg aaatattcgt
      181 cgtcagcgag tttgcctgaa atttttactc ccttcgaagt gcaaatgggc gcaacgcgaa
      241 ctctacgata ccgtcgtcct ccgtatttct gtggtgaaat ctgctggcct aattcgggag
      301 ctcacctttt tcgcgtaacg tcagaaactc tttcgcctgc atcggaaaca gcaccccgag
      361 cagaatatct tcatccgtct tggccaaggg tcccaagtcc ttgatcgact tctcccagtc
      421 ggagggatcg gccatttctc ccgagcgcct cgagtaatcc ggctctttgt ctttcataat
      481 cttccgaacc agatctttgt tgaccgagcc cggcggctgc ccgtactttc ccttgacgta
      541 atcgctggtc tcccggctga caatcttgta gcgctctccc gcaagcacgt tcaataccgc
      601 ctgcacaccg acgatttgac tggtaggcgt gagcagcggc ggatggccga gatctttctc
      661 cacgctgggc gtttctttta gcgccgcttc catcagatcc attcgccccg cttctttcag
      721 ctgagcgacc atgttggaaa tcattccgcc gggaactttg tgtataaaaa catcggcgct
      781 tacgccggtg tagccggttt cgtacttttc gtatttttcc ttgatctgcc gcgtaatccg
      841 cgccggctcc tccagaattt tcacatcgat gccggtatcg aacggcgtgc ctttcaacga
      901 cgccaccatc gtctcggttg ccggatgcgc cgaaccgaag gacagcggcg ataaaacggt
      961 gtcgatcatg tccactcctg cgaggatcgc catcagatga gacatactcg aagtcccacc
     1021 catatcgtgg caatgcagca gcactggtaa tttatctttg gcgcggtcct tgattccttt
     1081 gatgatgcga tagctgtcga acggcgtaag aattcccgtc gcatccttaa atgatagcca
     1141 atcggcgccg atctcgatca gctgctcggc atactccatc catttctcca gagtatgcac
     1201 gggactgact gtataattca cttcgacgtg cgcttcgccg cccatctcct tcaccgctcg
     1261 agtggcaact tcgatgtttc taaagtcatt ggtcgcttcg aagatccgaa aaacattcat
     1321 gccgttcgcg atggcgcgct cgacaaattt gtaaacgatc gtatcggtgt aaggccggta
     1381 ggcgagaata tgctggccgc gcaaaagcat ctgcagcttg gttttgggca tcaccgattt
     1441 gatgcgccgt agtcgctccc atggatcttc cttcaaatag cgcatacaga cgtcgaaagt
     1501 cgcgccgccc caaacttcca cggcgtgaaa cccacaggcg tcgagcgatg cgcagagcgg
     1561 gagtaagtcg tcggttgtga ctcgagtcgc gagcttgcac tgctgcccat cgcgcggcgt
     1621 gagatcggta atttcaactc tgcggctgcc gatcggttca ctgaggagct tgttgatatc
     1681 ggctgccaaa ttttccaaaa cttcttcacc catcgaatcg ctcctattct agacaaattt
     1741 agcatgcgta tggactgcaa ctatataggc taatcgctag tacagtaaaa gacgctgatt
     1801 ccggcacgca ggcgacttct atcctgctcc tattgaatcg gcttttgaac gcttcgaaca
     1861 gcttgaacat tttgaacaaa atcgccgcct tgcttttacg cactgattct cctacaaacc
     1921 ggctgatgaa gatcgtcaga ctctacaccg gtcaggacaa cgaatctcac ttcgaagata
     1981 ttgaggtcga gctcaatccc accggacgta tggaagcctc agaactgcaa cccacgcaag
     2041 gaatcatttt ccgttcggcg ccgccggatc atttgagtaa ttatcacccg gttcccaagc
     2101 gacaatacgt tattactctt tctggtcagg tcgaaatcga aactggtgat ggcacgatcc
     2161 gccgctttgg acctggcgac gtcatgcttg cggaggatac cactggccac ggccatatca
     2221 ctcgggtggt cggcggcgcg ccgcgcgatt acgtttttat cccgctcaaa taggatcaat
     2281 tatcaacccc gcaacattat cagcgtcgcc gaaacgcgac cgtgtcgccg tttgtcttat
     2341 tccctgctcg aacttcatgc catcaatgat ggtggcgact ctcggtatcg cgctgccaga
     2401 aattagagaa tcgttttcgc tttcggaact cgctgccggg agtcttttct cggtgatgat
     2461 gatcgttgcc gcgctaacca gcgctggcgc cggtcgcctt gctgatcgca tcggaccaaa
     2521 aaaggtgctg atcgctggat tgaccctgct tggagttggt tttgccgggg cggggatgag
     2581 cgagcattcg atcgtcttcg cttcttttct tgccgtgacc ggcatcggct acggcttcac
     2641 accgccgtcg ttatacctga tcatgtccga attgctacca cagaggcgcg gcctcggcgc
     2701 gagtctgatc tcagtcgctt acggcatcgg aggggcgatc ggcgcgctta tatcgagccg
     2761 agtagccgct c
//
LOCUS       DASULZ010000784         2955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_801185, whole genome shotgun sequence.
ACCESSION   DASULZ010000784 DASULZ010000000
VERSION     DASULZ010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801185"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFY96_16100"
     CDS             <1..297
                     /locus_tag="VFY96_16100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608049.1"
                     /translation="NRLIYTQKEKVIPIMIKATKKPQDAVSFAYDNLIKNCVWSVNTG
                     FSKDRTEWSVENAIENGDIPAEKKPSYDRIVDIKLGEEALKAAGGPTKIQGCAD"
     gene            378..1649
                     /locus_tag="VFY96_16105"
     CDS             378..1649
                     /locus_tag="VFY96_16105"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX5608050.1"
                     /translation="MPQYRFSEIKDRLDDWRVFNNMVDSPYYRDAVYEKFSPTEYERR
                     YQALRSKMHERGLNCVIVPGGPNHWSFGGGMLWLSGHWEWHAMANYVVFPLKGEPTLI
                     YSMGGTHIEATRRESAIKDVRQSRGGRFAEVMIERIKELGLEGGKIGILEVDPRFHDH
                     LPVNQYNALREALPNTTFSFESGLMHELVYKKSDEELECIRKAGKLCQAAMEALAERC
                     KPGATEYQLKAAAASAILEGGGDVDFAIIGSTPMKNPSMIFGNPRPSARVLNNGDIVI
                     MELAAGYRGYSAQIGSPICIGDPPTEILRFFDDIVLPGFEKMVSVARAGQHLNDLVEA
                     ARFYREKGVQSRPIHVHGIDFVSSEPHIFTDHVQAEEYEQVLQPGMVLVIEPNAVTAD
                     GMFGIFFGHTFIITEGEPECVDGYPWDLTIV"
     gene            1664..2743
                     /locus_tag="VFY96_16110"
     CDS             1664..2743
                     /locus_tag="VFY96_16110"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5608051.1"
                     /translation="MLAKHPDPTVDHVLKTWAPRMIVQGIDYNDLVTTGARILTWEDW
                     CREWCVTAAGHEEFARKAEAAGNRESATEAYLMASMAYHFGCNNSPQNLDEYVPAAKK
                     RVECYAKAAPYLNPPAVRHEVPFESFRMPAYLRVPAGVTKPPLVITISGLESTKEEAR
                     TMEDGFLRRGVATFTFDGPGQGETWFQGGLIVEFERATSAVIDYLEKLSAVDAGPIGV
                     FGPSMGGYLAPRSAACDRRIKACAFAGGMYDRTRVLNRLEDPFEFARIAHIWKVYDKK
                     KLVELHSRCTLEGLAAKIRCPLLVVHGTKDFVPVEQAKRIYDEASGAKEWLLLEGGNH
                     VCNNMPFRYRPAIWDFLARHLGGKV"
BASE COUNT          680 a          837 c          812 g          626 t
ORIGIN      
        1 aaccggctga tttacacgca aaaggaaaaa gtcattccca taatgattaa ggcgaccaaa
       61 aaacctcagg atgcggtgag ctttgcctac gacaatctga tcaagaactg tgtctggagc
      121 gtcaacaccg gtttctccaa agatcgcacc gagtggtccg ttgaaaatgc catcgaaaac
      181 ggcgacatcc ctgcggagaa gaagccgagt tacgatcgaa tcgtcgatat caaactgggt
      241 gaggaagccc tcaaagccgc aggcggaccg acgaagattc aaggctgcgc ggattagagg
      301 actgagtttt cgctcctcgc tcacccctca tgccccggtc ctcagtcctt aactacctga
      361 aaggcagcgc tactagtatg cctcaatatc gattcagcga aatcaaagat cggctcgacg
      421 attggcgcgt gttcaacaat atggtggact cgccctacta tcgcgacgct gtctatgaaa
      481 agttttcccc caccgaatac gagcggcgct accaagcgct tcgatcgaag atgcacgagc
      541 gcggtttgaa ttgcgtgatc gttcccggcg ggcccaatca ttggagtttc ggcggcggca
      601 tgctctggct ttccggccac tgggagtggc acgcgatggc aaactacgtc gtttttcctc
      661 tcaaaggcga gccgacgctg atctattcca tgggcggcac acacatcgaa gcgacgcgcc
      721 gggaaagcgc gatcaaagac gtacgccaat cgcgcggcgg tcgcttcgcc gaagtgatga
      781 tcgagcgcat caaagagctc ggtctcgaag gcggcaagat cggcatcttg gaagtcgatc
      841 cgcgctttca cgatcacctg ccggttaatc agtacaacgc attgcgagaa gcgctgccaa
      901 acaccacctt cagcttcgaa tcggggctga tgcacgagct ggtctacaaa aaaagcgatg
      961 aagagctcga gtgcatccgc aaagcgggca agctgtgtca agccgcaatg gaagcgctgg
     1021 ccgagcggtg caaaccaggc gcgacggaat accaactcaa ggccgcggcc gcgagcgcaa
     1081 ttctcgaggg cggcggtgac gtcgatttcg cgatcatcgg ctcgacaccg atgaaaaatc
     1141 cttcgatgat ctttggtaat ccgcgtccgt cagcccgcgt gctcaataac ggtgatatcg
     1201 tcatcatgga gcttgccgcc gggtatcgcg gctacagcgc gcagatcggc tcgccaattt
     1261 gcatcggcga cccgccaaca gagatactgc gctttttcga cgacatcgtg ctgcccggtt
     1321 tcgaaaaaat ggtttccgtc gcgcgcgccg gacagcatct caacgatctg gtggaagccg
     1381 cgcggtttta ccgcgagaaa ggcgtacaat ctcggccgat tcacgttcat ggcatcgact
     1441 tcgtcagctc tgaaccacat attttcaccg atcatgttca agccgaagaa tacgagcagg
     1501 tgctccagcc cggaatggtg ctcgtgatcg agcctaacgc ggtcaccgcc gacggcatgt
     1561 ttggaatttt tttcggtcac actttcatta tcactgaagg agaaccggaa tgcgttgacg
     1621 gttatccgtg ggacctgacg atcgtctgac aaggagaatt gctatgctcg ccaaacatcc
     1681 cgatccaacc gttgatcacg ttctcaaaac ctgggcgccg cgtatgatcg tgcaggggat
     1741 cgattacaac gacctcgtga ccaccggcgc gcgcatcctt acttgggagg actggtgccg
     1801 cgaatggtgc gtgactgccg cgggacacga agaatttgcg cgcaaggcag aagccgcggg
     1861 caaccgagaa agcgccaccg aagcctacct gatggcttca atggcctatc attttggctg
     1921 taacaattcg ccgcagaatc ttgacgagta tgttcctgcg gccaaaaagc gcgtggaatg
     1981 ctatgcaaaa gccgctccgt atctgaatcc tccagcggtg cgccacgaag taccgtttga
     2041 gagttttcgc atgcccgcct atctgcgcgt gccggcggga gtcacgaagc cacccctcgt
     2101 gattaccatc tctggcttgg aatcgaccaa agaggaagct cggacgatgg aagacggctt
     2161 tctgcgccgt ggcgtagcta ctttcacctt cgatggtcct gggcaaggag agacttggtt
     2221 tcaaggcgga ctgatcgtcg agttcgagcg cgcgacctct gcggtgattg attatctcga
     2281 aaagctctcc gccgtcgatg ccggtccgat tggcgtcttt ggccccagca tgggcgggta
     2341 tctggcaccc cgttcggcgg cttgcgatag gcgcatcaaa gcgtgtgcat ttgccggcgg
     2401 tatgtacgat cgcacccgtg tgctcaatcg tctcgaagat ccattcgagt tcgcccgtat
     2461 cgcgcacatc tggaaggttt atgacaagaa gaaattagtc gaactgcata gccgatgcac
     2521 cctcgaaggt ttggcagcga aaattcgctg cccgctgctc gtcgtccatg gcaccaaaga
     2581 tttcgtccca gtggaacagg caaagcgaat ttatgacgaa gcctccggcg caaaagagtg
     2641 gctattactc gaaggcggca atcacgtttg caacaacatg ccgtttcgct accgcccggc
     2701 catctgggat tttctcgctc ggcatctcgg aggaaaggtt tgattttcag ttttccggga
     2761 cgacagacat cgccttcgcc tctcacaagt caccgcacac aaacggccat tgactgaacg
     2821 agctgatccg atctagatta accgccgctt tacggaggag agcttatgct tcgtcggttc
     2881 ttctacggaa caatatttac agcccttctt ctcgtccgcg ctgccgatgc gcagccattg
     2941 ccgagatcca ttgcc
//
LOCUS       DASULZ010000785         4025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_91943, whole genome shotgun sequence.
ACCESSION   DASULZ010000785 DASULZ010000000
VERSION     DASULZ010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91943"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VFY96_16115"
     CDS             complement(<1..261)
                     /locus_tag="VFY96_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608052.1"
                     /translation="MRLSGLPPKQGLYDPRFEHDACGVGFVVHIKGERSHEIVDQALT
                     ILQNLDHRGACGCEENSGDGAGILMQVPHAFLKDACAGLGFEL"
     gene            complement(381..884)
                     /locus_tag="VFY96_16120"
     CDS             complement(381..884)
                     /locus_tag="VFY96_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013936616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX5608053.1"
                     /translation="MPNSINSFDETDLKILHILQLDGRKRLADIAEEVDLSAPAVMER
                     VKKLESSGIIRGYQALLDSKKVGKDITAFIGVSVGNQRDMDKFARQMMNYPDVLECHH
                     VTGDESFILKVKSANTASLEKLLGEIRSVEGVTRTVTRVVLSTAKEGQALELDADLVE
                     VAHAKKR"
     gene            complement(1107..1703)
                     /locus_tag="VFY96_16125"
     CDS             complement(1107..1703)
                     /locus_tag="VFY96_16125"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5608054.1"
                     /translation="MLERLKNSANSDDYFRWMLFVVGFYRGINNIDAATDLLEGFIKA
                     GKDAEQTAHCYLALGQMATDEQRLEDALKCFTLALELAPKRKKVAYVLHNNIGFCLNS
                     LARYSEGEAHCRDAIEVDWTRASAYRNLGISFQGQGNARAAAWALVEAVRADPADQRA
                     RAILHKLVTLIPSLPVECPWAVDAVESDLTADPDLPLN"
     gene            2458..2976
                     /locus_tag="VFY96_16130"
     CDS             2458..2976
                     /locus_tag="VFY96_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608055.1"
                     /translation="MSHAKSLRVVGQCLEYAHIAIFELEKYGPQYMLWSEAVNEAGET
                     ALRNFLNDRLEFSNETRHHSPKRVFVFSPADIARLDAQAQRQRKNRSSQAMPPYKIVS
                     HGLRILGEHLDRIQANAFRIEWLSGSVIMDHQRLDGARSFKRFTFEEIHRLDLQPRLR
                     RSSLYLFPPVHS"
     gene            complement(3035..>4025)
                     /gene="ydiK"
                     /locus_tag="VFY96_16135"
     CDS             complement(3035..>4025)
                     /gene="ydiK"
                     /locus_tag="VFY96_16135"
                     /inference="COORDINATES: protein motif:HMM:NF008216.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HEX5608056.1"
                     /translation="FLGAIVWATTVVVATWPFMISLQTVLWNKRSLAVMVMTLLLLCV
                     LVVPLSFAVAAIVSNLDDIAVWAKNLATFKLPPPPVWLASLPLVGAKAVELWDRVAAA
                     GVQDATVRVAPYAGGVIKWFAAQLGSVGVLLLEFLLTVVLAAAMYAGGERAAQRLFRF
                     GQKLAGSGGENAIYLAVQTIRGVALGVVVTAIAQSLFAGIGLIIAGVPLAGALTGAML
                     LLSIAQIGVSPVLGAVAAWLYWIGESAWGTFMLVWMFVAGTMDNFLRPVLIRKGADLP
                     LLLVFAGVVGGLVAFGFIGIFVGPVILAVADKLLVAWIDSDMQPADAGDRQSG"
BASE COUNT         1013 a         1125 c          981 g          906 t
ORIGIN      
        1 cagttcaaac cccaaaccgg cgcacgcgtc ttttagaaag gcatgcggca cctgcatcaa
       61 gattcccgcg ccgtcgccac tgttctcctc gcagccgcac gcgccgcgat ggtcgagatt
      121 ctgtagaatg gtcagtgcct gatcaacaat ttcatgggac ctttcccctt tgatgtgaac
      181 cacaaacccg acgccacaag cgtcgtgctc aaaacgagga tcgtaaagtc cttgcttagg
      241 cggcaagccg gataatctca tgatgcttca ccctttctgc gagtcgccgt tcaaagtcca
      301 agtgcaacgt tcaatcgcga tcgagcacag taccgcgctg gggacttgag ttcgcaacgt
      361 gagaacggct tcacacggcg ttatcttttt ttcgcgtggg caacttccac caggtcagcg
      421 tcaagctcga gcgcttgccc ctctttagcc gttgacagca cgacccgtgt aacggtccga
      481 gtcacaccct cgaccgaacg aatttcccct aacagttttt ccaacgaagc ggtgttcgcc
      541 gatttgacct ttaaaatgaa actttcatca ccggtaacgt ggtgacattc caaaacgtca
      601 ggatagttca tcatctggcg cgcaaattta tccatatcac gctgatttcc aacggaaacg
      661 ccaataaacg cagtgatgtc cttgccgact tttttgctat ccaacagcgc ttgatagccg
      721 cgaataatcc cgctcgattc gagcttctta actcgctcca tcaccgccgg cgcagacaaa
      781 tccacttctt cggcaatatc ggcaagtcgt ttacgcccgt ccaattgcag gatatgtagt
      841 atttttaaat cagtttcatc gaatgagtta atagaattag gcattgggcc tgttttggtt
      901 aataaactta atgtcagttg agttttcgaa tgtcaagcga tttcgcgaca aattttggat
      961 ttttcggctg aatcaccgcc ttccacgaca tatgggcgac ggctctcgaa ctgagcacgg
     1021 cgaccgaagc cgcgctatgg ttgcgcagtc agaattagcg ggactctatc ggatgcagcc
     1081 ggcgggagaa gtctaaatgg aagcgatcaa ttgagtggaa ggtcgggatc agcggttaag
     1141 tctgactcta ctgcatccac cgcccacggg cattctactg gaagactggg gatcagcgta
     1201 accagcttat gcagaatcgc ccgcgcgcgt tgatctgctg gatcggcccg gaccgcttcg
     1261 accaaagccc acgcggcggc gcgagcatta ccctgacctt gaaaactgat gccgagattc
     1321 cggtaggcgc tggcgcgcgt ccagtcgact tcgatcgcgt cgcggcaatg cgcttcgccc
     1381 tcgctgtaac gggccaagct gttcaggcaa aagccgatgt tgttatgcag cacgtaggca
     1441 accttttttc tcttcggggc gagctccaat gccaatgtaa agcacttcaa cgcgtcttct
     1501 aatcgttgtt cgtccgtggc catttgaccc agagcgagat aacaatgagc ggtttgctct
     1561 gcgtccttgc cagccttaat gaatccttca agcaaatcgg tagctgcatc gatattatta
     1621 atcccacggt aaaagccgac tacgaacagc atccagcgaa aataatcatc gctattggcg
     1681 ctgttcttca gtcgttctag caaaatggct tcggtgtcct gcgattgttc cgagactgcg
     1741 agcgctgttg ttttcttgtc tgttggattc attaatcgag caaaaagcaa ctccaatacc
     1801 atccagtctt acgaaaagaa agccaaaaac aatgaaaatt cggcagcaaa tcacgtgtgt
     1861 tcgttgtgtg gatgtcccca acggttaaag acttaaaaaa gaaggcaagg cggcccggtg
     1921 agaaaactgt agcattagaa gagccgctga aaaatacagt tggttgcgct gttctcgtgt
     1981 taagaatttt tacagttaga gcatccctgt tatcgagacc ggccgctcat tcgcttcgct
     2041 aaatacaatg gagtttttgt tgaactgcag agaacgaccg aagctcaaac tctcaatggc
     2101 ggccggcgag cgtcacaccg cgtttttgta tgcactgcca gctctgttga accgtcggtg
     2161 ctacaatcac tcagctattt cgttccttac gacaacgtga aggctgttcc cagtttggct
     2221 gccggacgca gctgaataca gtgcgaaacg gtacaaaatt gtgagttgca atttttggcg
     2281 cggccgatga tgttctgcga caccaaagct caccagagga agatcgcata attagcccga
     2341 cccagattga gtaaaccgtc gcatttgtag cgcattcacc tcggcaaaca ttctggtatc
     2401 acagttgctc acacgaatac gggtgcaatt attatgtatg tttaagtagt caactttatg
     2461 tcgcatgcca aatcgttaag agttgttggg cagtgtctag aatacgcgca tatcgcgatc
     2521 ttcgaattgg agaagtatgg cccgcaatac atgctgtgga gcgaagccgt caatgaagcc
     2581 ggcgaaacgg ctttgcgaaa ttttctcaat gatagactcg agttttcgaa tgagacccgt
     2641 caccactcgc ccaagcgagt gttcgttttc agcccagcgg acattgcacg gctggatgct
     2701 caggcacaac gacagagaaa aaatagatca agccaagcca tgccgcccta caaaatcgtc
     2761 tcgcatggtc tacggattct tggtgagcat ctagacagaa ttcaggcaaa cgcttttcgc
     2821 atcgaatggc ttagcgggtc cgtaatcatg gatcatcagc ggctcgatgg ggcgcgtagt
     2881 ttcaaaagat ttacattcga ggagattcat cggctcgatc tacaaccccg gctgcgccgc
     2941 tcgagtcttt acctttttcc tccagtgcac agctgagtgg gcgcttctcg accttgtttg
     3001 cgcagcgcgc gctcctctgc gtgatccccc gcggttaacc agattgcctg tcacctgcgt
     3061 ccgcgggctg catatcgctg tcgatccacg ccacgagtag cttgtcagca actgccagta
     3121 tgaccgggcc gacaaaaatt ccaatgaaac cgaaagcgac aagaccaccg acgacaccgg
     3181 caaaaacgag cagcaacggc aagtccgcgc cttttctgat cagcacggga cgcaaaaaat
     3241 tatccatcgt cccggcgaca aacatccaca ccagcatgaa tgttccccag gccgattcac
     3301 cgatccaata cagccacgct gcaaccgcac cgagaacagg actcactccg atttgtgcaa
     3361 tggacaaaag cagcattgca ccggtgagag cgccagcgag cggaacgccg gcgatgatca
     3421 acccaatccc ggcaaaaagc gactgtgcga tcgcagtcac gacgacgccg agggcgacac
     3481 cccggatcgt ctggacagct aaatagatcg cgttttcacc gcctgaccct gccagttttt
     3541 ggccaaaacg aaaaagccgc tgtgctgccc gctcgccgcc ggcatacatg gcagccgcta
     3601 gcacaacggt caacaaaaat tccagcagca atacgccgac gctgcccaac tgcgccgcaa
     3661 accatttaat cacgcccccc gcataggggg cgaccctgac cgtcgcatcc tgaactcctg
     3721 ccgcggcaac gcggtcccat agctcgacag ctttcgcgcc aactagcgga agacttgcta
     3781 gccaaaccgg tggcggcggt aacttaaacg tcgccaagtt tttggcccag accgcaatat
     3841 catcgagatt ggatactatc gcggctacgg caaaactcag cggcacaacc aaaacgcaga
     3901 gtaacaatag ggtcataacc attaccgcca agctccgctt gttccataac acagtttgta
     3961 gcgaaatcat gaaaggccat gtggctacaa ccaccgtcgt cgcccagaca atggcgccga
     4021 gaaat
//
LOCUS       DASULZ010000786         3881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_700407, whole genome shotgun sequence.
ACCESSION   DASULZ010000786 DASULZ010000000
VERSION     DASULZ010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700407"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2581
                     /locus_tag="VFY96_16140"
     CDS             <1..2581
                     /locus_tag="VFY96_16140"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5608057.1"
                     /translation="RSANGNGVRARWSHYQSLLDSATLSIVETDLSGRIVDCNPVLAN
                     LLRSAPEKVKHRKFVTLASEKSRSKEIATRDEVLERGSTDEYEIELMREDGTAFPVII
                     KKWLQSNRYGKAQRVWMLVRDITEKKKNGDAFYRHVILLLENQLRNQSLLAGAPIGIL
                     TIDGNGRIESVNPAAEELLRAKSDEVIRKRLDEFVTSPSGKPLSIAPGQPQTAVAKWQ
                     NGGILPVQVTFSELRTQDELLFTCFLRDMTGDMQIRETLQRAHDDLADRVREGTEEIA
                     RLFDALQTEINERTWVQEQFRVAVESAPNGILILNQEGRIVLLNAHVEQIFGYGREEL
                     LGRPFELLVAEKFRSVLPHPPENCLAQAQVNWMGRGQELYGLRKDATEFPVEIGLNPI
                     HTPTGAGLLVSVIDVTERKRVESELKRSAFHDGLTGLPNRTLFLDNLLRLNAAAKRHG
                     HHPFALLFLDLDRFKVINDSLGHSAGDKLLIEMSQRLVECTREEDTVARLGGDEFALL
                     LEQVRGPEDAVRVAERVLERLADPLVLDGNEVSITGSIGIALSLTAEKNPEDLLRDAD
                     MAMYQAKTRRLGYQIFDAKMHARALDRMRLEIDMKRAIERQQIQLHYQPIISLETGRL
                     AGFEALARWHHDGRGAIPPAQFIPLAEETGLVTPLSRSVFRQACAQVHDWRTRFSLSD
                     DFFVSVNVSSKQLSHRDLIDQLDRILQETSLDPEYLRLEITESALAENTRVASYTLAQ
                     LRKRRIKLCLDDFGKGFSSLNYLHRFPIDVLKIDRSFVHRLSAGFSNDSGKKRPYEII
                     RTIVALAQILGMQVVAEGVEVADQAAILKELGCEFGQGFLFSPAVDAGGAARFFDHAS
                     RAS"
     gene            complement(2590..2823)
                     /locus_tag="VFY96_16145"
     CDS             complement(2590..2823)
                     /locus_tag="VFY96_16145"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX5608058.1"
                     /translation="MIVCNHADVQGQHLNNTVMYTPVYAGKDRETLIGFFAINVHWID
                     IGGITPRSSDIYMEGLQLRSIKLWSKGEPIQEV"
     gene            complement(2820..3119)
                     /locus_tag="VFY96_16150"
     CDS             complement(2820..3119)
                     /locus_tag="VFY96_16150"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX5608059.1"
                     /translation="MNPNLKSTSTENSVDPITLEVIRHGLVSITDQIDANISRTAFSP
                     YLYEYKDFAVGLVSPEGELVAQCTGGMPVFVADSVGMAVRDGLTVYGRARLHRAT"
     gene            complement(3194..>3881)
                     /locus_tag="VFY96_16155"
     CDS             complement(3194..>3881)
                     /locus_tag="VFY96_16155"
                     /inference="COORDINATES: protein motif:HMM:NF039581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX5608060.1"
                     /translation="GPLHSCALAHELSIPTVIIPPEPGNFSAIGMLLADARIDTSKTF
                     VGILNDQSVPSMAEIYKAMQHEAADSLEKEFGTRDIFFEHYAEMRYRGQRHNIKVPVS
                     GLKDAEQIRVAFERDYKRRYGHADAKAPAEFQALHLSAFARLKQPEIARFPRAASKAQ
                     QPTERRVYLGGAGGWIDAKIYHRDALEAGFNGSGPTVIEEYGSTTVVWPGDRFEIGQL
                     GEIRIQCAAK"
BASE COUNT          874 a         1093 c         1090 g          824 t
ORIGIN      
        1 gcgaagtgca aacggcaacg gagtacgcgc gagatggtcg cattatcagt ctttgctcga
       61 ctcggcaacg ctgagcatcg ttgaaaccga tctctccggg cgaatcgttg attgtaatcc
      121 agttctcgcc aacttgcttc gttcggctcc ggaaaaagtg aagcaccgca aattcgttac
      181 gctggcctcc gaaaaatcac gctcgaaaga gatcgccacc agggatgagg tgctcgaacg
      241 gggcagtacg gacgagtatg aaatcgagct gatgcgggag gacggtacgg cattcccggt
      301 cattataaaa aaatggctgc agagcaacag atacggaaaa gcgcaaagag tatggatgtt
      361 ggtgcgggat attaccgaga agaaaaaaaa cggtgatgcc ttttatcggc acgtgatcct
      421 gctcctagaa aaccagctgc gcaatcaatc gctgctcgcg ggcgctccca tcggaattct
      481 gaccatcgat ggaaacggcc gaatcgaatc ggtgaacccg gcggcggagg aattgttgcg
      541 cgcgaaatct gacgaggtca ttcggaagag actcgacgag ttcgttacca gccccagcgg
      601 caagcctcta tcgattgcgc cgggacaacc ccagacagct gttgcaaaat ggcagaatgg
      661 cggaattttg ccggtccagg tcaccttcag tgagctacgc acgcaagacg aactcctgtt
      721 tacatgtttt ctgcgcgaca tgaccggcga catgcagatc cgtgagactc tgcagcgagc
      781 tcacgatgat ctggcggatc gcgtgcgaga gggaaccgaa gagatcgcgc gtttattcga
      841 cgctttgcaa acagaaatca atgaacggac gtgggtacag gagcaattcc gggtcgcggt
      901 cgaatcagcc cccaatggaa tcttgatttt gaatcaggag ggacgaatcg tgctcctgaa
      961 tgcccatgtc gagcagatct tcggttatgg acgcgaagaa ctgctggggc gtccgttcga
     1021 gctgctggta gccgaaaagt ttcgcagcgt tctgccgcat ccccctgaga attgcttggc
     1081 ccaggcgcaa gtcaattgga tgggccgcgg ccaagagctc tacggtctgc gcaaagacgc
     1141 gactgaattc ccggtggaaa tcggtctcaa tccgattcac acgcccaccg gcgccggatt
     1201 gctcgtttcg gtgatcgatg tcaccgagcg caaacgcgtc gagtcggagc tcaagcggag
     1261 cgccttccac gacggcctca cgggcctgcc caatcgcacc ctgtttttgg ataacctgct
     1321 tcggctcaat gcggcggcaa agcggcacgg ccatcatcct ttcgcgctcc tgttcctgga
     1381 tctcgaccgc ttcaaagtca tcaacgacag cctcgggcac agcgctggtg acaaactgct
     1441 gatcgagatg tcacagcggc tggtggaatg cactcgcgaa gaagacaccg tcgcacgctt
     1501 gggcggcgac gaattcgctc tccttctcga gcaggtgcgc ggtccggaag atgcagttcg
     1561 cgtcgccgaa cgagtgctcg agcggctcgc cgatccttta gtgctggacg gaaacgaagt
     1621 atcgatcacc ggcagcatcg gcatcgcgct cagtcttacc gccgagaaaa atcccgaaga
     1681 cctgttgcgc gatgccgata tggcgatgta tcaggccaaa actcgccgtc tgggctatca
     1741 gattttcgac gcaaaaatgc acgcgcgcgc gctcgatcgc atgcgtctgg aaatcgacat
     1801 gaaacgggcg atcgaacggc agcagattca actgcactat caaccgatta tttctttgga
     1861 gaccggcagg cttgccggct tcgaagcgct cgcccgctgg catcacgacg gccgcggagc
     1921 aattcctccg gcgcaattca ttccgcttgc cgaagagacc ggactggtga cgcccttgag
     1981 tcgatcggtt ttcaggcagg cctgcgcgca agtgcacgat tggcgaacgc gcttcagctt
     2041 gagcgatgat ttttttgtca gcgtcaacgt ttccagcaag cagttgtccc atcgcgattt
     2101 gatcgaccag ctcgatcgta ttttgcagga gaccagttta gatcctgaat atttacgtct
     2161 cgagatcacc gaaagcgctc tggcggagaa cactcgggtc gcatcgtaca ctctcgcaca
     2221 actcaggaag cgcagaatca agctctgcct ggatgatttc ggcaagggat tttcttcgct
     2281 caattatctg caccgatttc ccatcgatgt cttgaaaatc gaccgttcgt tcgtgcatcg
     2341 gttgagcgct ggctttagca atgactccgg aaaaaagcgg ccctacgaga tcattcgaac
     2401 catcgtggct ctggcgcaga ttctcggcat gcaagtggtt gccgaaggag tggaagtcgc
     2461 cgaccaagca gccatactga aggagctcgg ttgtgagttt ggccagggtt ttcttttctc
     2521 tcccgcagtc gacgcaggcg gcgcggcgcg tttttttgac cacgcctcga gagcgtctta
     2581 ggtcgacgtt tagacttctt gaatcggttc gcccttcgac cagagcttga ttgaacgcag
     2641 ttgcaaacct tccatgtaaa tatcgctgga gcgtggtgta attccgccga tgtcaatcca
     2701 atgcacgttg atggcaaaaa agccgatcag ggtttcccga tccttgcccg cataaacggg
     2761 cgtgtacatc accgtattgt tgagatgctg gccttgaacg tcagcgtggt tgcagacaat
     2821 cacgtcgccc gatgcaaacg cgcgcggcca tagaccgtga gcccatcacg caccgccatg
     2881 ccgacggaat cggcgacaaa taccggcatc ccgccggtgc attgcgcaac gagttcaccc
     2941 tccgggctca cgagcccaac cgcaaaatct ttgtactcgt agaggtatgg gctgaacgcg
     3001 gtgcgcgaaa tgttcgcgtc gatctgatcc gtgatcgaga ccaaaccgtg gcgaatcacc
     3061 tcgagcgtga tcggatcaac ggagttttcg gtcgaagtag attttagatt agggttcata
     3121 cgccgagaac agatcggatt gcatcggaat gttcaaaaga catcccgata agcaaatctt
     3181 tcccggacac aactcacttc gccgcgcact gaatccggat ctctcctagc tggccgatct
     3241 cgaaccggtc gcccggccat accacggtcg tcgagccgta ttcttcgatc actgtcgggc
     3301 cactgccgtt gaagcctgcc tcgagcgcgt ccctatggta aatcttcgcg tcgatccaac
     3361 caccagcacc gccgagatag actcgccgct cggtcggttg ttgcgctttg ctcgccgcgc
     3421 gcgggaaacg cgcgatttcc ggctgcttga gccgcgcaaa agcggacaaa tgcaaggcct
     3481 gaaattcggc cggcgccttg gcatccgcat gaccgtagcg ccgcttataa tcgcgctcga
     3541 aggcgacacg gatctgttcc gcgtccttca atcccgaaac cggaacttta atattgtgcc
     3601 gctgcccgcg gtagcgcatc tcggcataat gctcgaaaaa aatatcccgg gtgccgaatt
     3661 ccttttccag cgaatctgcg gcctcatgtt gcattgcttt gtaaatttca gccatggaag
     3721 gcacgctttg gtcgttcagg attcccacga acgttttgga ggtgtcgata cgcgcgtcgg
     3781 ccagaagcat accgattgcc gaaaaattcc ccggctccgg tggaataatg accgtgggaa
     3841 tcgagagctc gtgcgcgagt gcgcacgagt gcagcggccc g
//
LOCUS       DASULZ010000787         2004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_904278, whole genome shotgun sequence.
ACCESSION   DASULZ010000787 DASULZ010000000
VERSION     DASULZ010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904278"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..517
                     /locus_tag="VFY96_16160"
     CDS             167..517
                     /locus_tag="VFY96_16160"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5608061.1"
                     /translation="MARTGLSHIALKSRDLKKTEEFYVGVLGCKVAFRHPPDMIFLTT
                     PGSGDLLNFVKSAGRISGSQGLEHIGFKMSAAELKKMEKTLKRHGFKIDGRRGREAFY
                     FTDPNGYQIEYYCD"
     gene            complement(752..1804)
                     /locus_tag="VFY96_16165"
     CDS             complement(752..1804)
                     /locus_tag="VFY96_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEX5608062.1"
                     /translation="MNAPPVRGRGSSTNPKNRFESVERVFEPPENEDETSSPLTQFFP
                     DHSKSIIAYNDSPDVGFDASINPYRGCEHGCVYCYARPTHEYLGFSSGLDFETKIMVK
                     EDAPELLRKELSSRSWKPQLIALSGNTDCYQPVEKKKQLTRRCLEVLLEFRNPVVIIT
                     KNYLVTRDIDVLRELARYDCVGVTVSLTTLDLRLSSLLEPRASRPARRLAAIRALAEA
                     GVPVGYLQAPMIPGLTDAEAPGIAAAAAEAGATFCGYVALRLPFAVKTLFEQWLEQHY
                     PEKKNKVLNRVREIRDGKLNDPNFTTRMRGSGIFAEQMAELFQLAQKKSGINKRWPKL
                     TTEHFRRPARDQLALF"
     gene            complement(1817..>2004)
                     /locus_tag="VFY96_16170"
     CDS             complement(1817..>2004)
                     /locus_tag="VFY96_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608063.1"
                     /translation="KQDIRELQKQKAELLSSNGDRQKVKRLRRKVKLMKRVTRHLARD
                     AKLAAAAAAAKPATPAS"
BASE COUNT          458 a          527 c          564 g          455 t
ORIGIN      
        1 gaacgctcaa cagtgacaat tgggaccgaa aaactaacaa gccctataga tggaaaacta
       61 gactgttttc cttaaaagca atcgaagcag actgatagga cgggattgat cgtgcgctgc
      121 tcaggcgata caagatgtca ctgcgtcgat ttcaaaagga gactacatgg cgcgcacggg
      181 tttaagtcac atcgctctta aaagccgcga tttgaagaaa accgaagagt tctacgtcgg
      241 tgtcttgggc tgcaaagtgg ccttccgtca tccgccagac atgattttct tgacgactcc
      301 cggcagcggc gatctattga atttcgtgaa aagcgccgga cgtatttccg ggagccaagg
      361 gctcgagcac atcggcttta aaatgagtgc agcagagctc aagaagatgg aaaaaaccct
      421 caaacggcac ggcttcaaga tcgatggcag acgtggtcgg gaggcttttt attttactga
      481 tccgaacggc tatcaaatcg aatactactg cgactgagct tcgtcgccgc ggcaaccacg
      541 gatgaaaatt cctggttcca gattggttga aatcgttccg ttcaggcgaa cgaacggtcc
      601 gtgatgccgg caggccggca tccatggtca tgaaacacgc cgatacttta tacgctggct
      661 tccgctttcg ccggaatgac tgaacagaag ttgcgtaaag tcaaagctcg cgaaagtttc
      721 gatttatgca actgaatatt aaacgatgaa atcaaaaaag cgctaattga tcccgtgcgg
      781 gtctgcgaaa gtgttcggtg gtgagcttgg gccatcgctt gttgatgcct gattttttct
      841 gagccagctg aaacagctcc gccatttgtt cggcgaaaat accgctgccg cgcattcggg
      901 tggtaaagtt tgggtcattg agcttgccat cgcggatctc gcgcacgcga ttgagcacct
      961 tgttcttctt ctcaggatag tgttgctcga gccactgctc gaaaagcgtt ttgacggcga
     1021 acggtaaacg cagcgcgaca tagccgcaaa atgttgcacc tgcctccgca gcagcggcgg
     1081 cgatgcccgg agcttcggcg tcggtgagtc ccggaatcat cggcgcctgc agataaccga
     1141 cgggtacgcc ggcctcggca agcgctcgga tcgcggcaag ccggcgtgcc ggccgcgacg
     1201 cccgcggctc gagtagtgaa gacagacgga gatcgagggt ggtgagcgaa actgtcacac
     1261 cgacgcaatc gtagcgggcg agttccctca ggacatcgat atcgcgggtg accagataat
     1321 ttttcgtgat gatcaccacg ggattacgaa attcgagcag aacttccaag cagcgccggg
     1381 tcagctgctt ttttttctcc accggctggt aacagtccgt attgccgctg agagcaatga
     1441 gttggggctt ccaggatcgg ctcgataact ctttgcgcaa aagttccggt gcatcctcct
     1501 tcaccataat tttggtctcg aaatccaaac ccgacgagaa cccgagatat tcgtgcgtcg
     1561 gccgtgcata acagtagacg cagccgtgct cacagccacg gtagggattg atgctcgcgt
     1621 caaacccgac atccgggcta tcgttgtagg cgatgatgct cttggagtgg tccggaaaaa
     1681 attgcgtcag cggcgatgaa gtctcgtctt cattctccgg tggctcgaac acccgctcca
     1741 cggattcgaa gcggttcttt ggattggtgc tcgagccgcg tccgcggacg ggaggcgcgt
     1801 tcatgaaaag atccgatcaa ctcgccgggg ttgccggttt cgccgcagct gcggccgccg
     1861 ccaattttgc atcgcgcgcg aggtggcgcg tcactcgttt cattagcttg accttgcgcc
     1921 tgagccgctt gactttctgg cgatccccgt tggagctcaa aagctcggcc ttctgttttt
     1981 gtaactcgcg aatatcctgc ttga
//
LOCUS       DASULZ010000788         2375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1208462, whole genome shotgun sequence.
ACCESSION   DASULZ010000788 DASULZ010000000
VERSION     DASULZ010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208462"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VFY96_16175"
     CDS             <1..371
                     /locus_tag="VFY96_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608064.1"
                     /translation="LATSRKYMRANPQLVENLAKAVLEGVAFVQNPANKKIVIGSIAR
                     NLRLDGSERLERAYKTISESFPRKPCPSLPGIASVLRLMAQHGLNPRAVEMKPQDIAD
                     MSFCKKLDDTGFFERLHQSG"
     gene            758..1144
                     /locus_tag="VFY96_16180"
     CDS             758..1144
                     /locus_tag="VFY96_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608065.1"
                     /translation="MMSQDYISNREFADDRFSQEEKRRMFAQPDVLCVHEYLHVYQCR
                     PAETPERRLLAAVLRDAIDCYLRDCCAKNRHRKRSFREAEEWFFGSSADGIFSLESVC
                     GILNIDPGYIRRSLLRHKDKELLQAA"
     gene            1369..>2375
                     /locus_tag="VFY96_16185"
     CDS             1369..>2375
                     /locus_tag="VFY96_16185"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015192164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5608066.1"
                     /translation="MDHVVIVGGGFGGLSAAQSLKNVPVRLTLLDRRNFHLFQPLLYQ
                     VATGWLSPANIASTLRATLRHQKNASVLLAEAKDFDVEKCRVLLDDGEIQYDTLVLAT
                     GSGTHYFGNDRWALYAPGLKTVEDATEIRRRILKAFEAAERQNDPAKRKALLTFAIVG
                     GGPTGVELAGTLGEIAHDTLKNDFRNIDPSTTTILLIEGGERIIPSFDPTLSEKAAST
                     LQTLGVTVLRNVLVTDVGAEFLTIKRGNTPERIACHTVIWAAGVRASPHGQLIASKTG
                     AKTDRGGRVEVEPDLTVKGHPEIFVIGDLANYSHQNGKPLPGVAPVAIQQGRYVAQVI
                     AA"
BASE COUNT          556 a          654 c          648 g          517 t
ORIGIN      
        1 gcttggccac ttcgcgcaaa tatatgcgcg ccaatccgca actggtcgaa aacctcgcca
       61 aggcggtgct ggaaggtgtc gcgttcgtgc aaaatcccgc caacaaaaaa atcgtaatcg
      121 ggagtatcgc gcgcaattta agactcgatg gaagcgagcg cctggagcgc gcatacaaaa
      181 ctatttccga gagttttccg cggaaaccgt gcccaagcct gccgggaatc gcatccgttt
      241 taaggctcat ggcgcagcat ggactcaacc cccgggcagt cgagatgaag ccgcaggaca
      301 tcgccgatat gagtttctgc aagaaactgg acgatacggg gtttttcgag cggctgcatc
      361 aatcaggatg aagggtcagg tatttgtcgt ccacagaggg aggcatgtct gacgaaggga
      421 cgcatgcccc aaacgctacg ccgttagtaa ccgagggcgc tcgtcgaccg agacgacggt
      481 cgcatttaca aactgcgtag ttcccgctgc aacttctctc cgtgtaagcc tttccctcac
      541 agctgtcgga ctttcccttt cgtgcctgat tcgccagccc caaagcgaaa ttgtttttct
      601 aaaaatttca aggcgcacga gcgaacgggc tctcggtaca agagttgcac catcggggta
      661 ccgcggtcgc agagctttct gtgatgataa ataaatatta aaaggccgct acacgcttct
      721 atcatacgca tgcaggcggg cggttgaggt aacacccatg atgagtcagg actatatcag
      781 caatcgggaa tttgccgatg atcgtttctc gcaggaagag aagcgtcgaa tgtttgctca
      841 acccgatgtt ctatgcgtgc acgagtacct gcacgtgtat caatgccgtc ccgctgaaac
      901 accggagcgc agattgctcg ccgccgttct tcgtgacgcg atagattgct atttgcggga
      961 ttgttgcgcg aaaaaccgcc atcgcaagag aagctttcgc gaggctgagg agtggttctt
     1021 tggatcgagc gctgatggaa tttttagtct ggaaagcgtc tgcgggattt tgaatatcga
     1081 tcccggttat atccgaagaa gccttttgcg gcacaaagac aaggaactgc tccaagcggc
     1141 gtagttgtca acctgatcac agcgtaaagc gcaagggcgc cgctccttca tccacacgcg
     1201 ctgaacgccc tgacgcgaga gccggaaaca tgctgtgatc gaaatccccc gatgtcttcc
     1261 ttttccgaag gggagatgga gggggatttt gtttttggtg gcctgcctgc agcagtccgg
     1321 catctctagc gtttgcagcg ctgtggatgt gcccctccct acgtcatcat ggaccatgtt
     1381 gtcattgtcg gcggtggttt tggtggactc tccgccgcgc aatcattaaa gaacgttccg
     1441 gttcggctca cattgctcga ccggcgcaac tttcaccttt tccaaccatt gctctatcag
     1501 gtagccaccg gttggttgtc tccggcaaac atcgcttcaa ctcttcgggc cacgctcaga
     1561 caccaaaaaa atgccagcgt tcttttggct gaggccaaag atttcgatgt cgagaagtgc
     1621 cgcgtgctgc tcgacgacgg cgagatccaa tacgacacgc tggttctcgc aaccggatca
     1681 gggacgcact atttcggcaa tgatcgttgg gcgctttatg cgccgggcct caaaacagtc
     1741 gaagatgcca cagaaatccg ccggcgaatc ttgaaagcgt tcgaagccgc ggaacgacaa
     1801 aacgatcctg ccaagcgcaa agctttgctt acctttgcaa tcgtcggcgg tggcccgacc
     1861 ggcgtcgagc tcgcgggcac tctgggtgaa attgcccacg acacgctgaa aaatgacttt
     1921 cgcaatatcg atccctcgac gacgacgatc ctgctgatcg aaggcggcga gcgtatcatt
     1981 ccgagcttcg atccaacact ctcggaaaaa gcagctagca cactccaaac gcttggcgtt
     2041 acagtgctcc gaaacgtgct ggttactgac gtcggcgcag aattcctcac aatcaaacgt
     2101 gggaacaccc ccgagcgcat cgcttgtcat acagtgatct gggcggcagg agtgagagcg
     2161 tcgccgcacg gccaactcat tgccagcaaa accggcgcta agaccgaccg tggcggtcga
     2221 gtcgaagtcg agccggatct cacggtgaaa gggcatccag aaatattcgt tattggtgat
     2281 ctggccaact attcacacca gaacggaaaa cctcttcctg gcgttgcgcc agtggcgatt
     2341 caacaaggtc ggtatgtcgc acaagtgatc gccgc
//
LOCUS       DASULZ010000789         5219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_599740, whole genome shotgun sequence.
ACCESSION   DASULZ010000789 DASULZ010000000
VERSION     DASULZ010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599740"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VFY96_16190"
     CDS             <1..497
                     /locus_tag="VFY96_16190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608067.1"
                     /translation="QYVSIPGSQPKIAALQQGIIQGALLAPPSDYVALKAGFKRLVNL
                     ADLFKDTSFSGLAATGKTVRENPQFVKRMVRAVVRGVIHSRDYPEDAIQTMMKHWHMD
                     RDVSVDAYNLVKEALIPVPTEKGVELMAHWQSIALNVPPKRPARDYMDLRFVKEVMTE
                     LEKK"
     gene            508..1104
                     /locus_tag="VFY96_16195"
     CDS             508..1104
                     /locus_tag="VFY96_16195"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5608068.1"
                     /translation="MRKIVFLAALLLSATISNAAETPEKKSPLYATLKTSMGDIVIQL
                     FEDKAPKTVANFVGLASGTKDWIDPKTGDKVKRPLYNGTIFHRVIPGFMIQGGDPLGN
                     GTGGPGYKFEDEFSPELRHNKPGILSMANAGPNTNGSQFFITHKETPFLDGKHSVFGE
                     VVKGQEVVVAIANVPRDGRDRPNKDVVIKEVVITRGSY"
     gene            1127..2122
                     /locus_tag="VFY96_16200"
     CDS             1127..2122
                     /locus_tag="VFY96_16200"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5608069.1"
                     /translation="MNRRTCGLEKICRAALLVLFALSGLLRPSLAAEPALLQTTAVYG
                     VIATDPLYLWIAQDKGLFQKNGLQVDLTHIPTNQGVQALVAGKVNFVTAGPQILEANL
                     AGSDTVYIMSPVNAFVFSLYSQPAIANIKALAGKTFGATNKGTPSDIAAHMLLTKNGL
                     KPDIDVKFAYLKEIPALVAALQQGIIDAALLTPPATLTARNFGLKELLNVTELKIPFV
                     QHAIGTTRSYISANPEVVRRFVRSAAEGLDFLRRNRTDAVAIMSKYTKISDPAQLNEG
                     LDAYDKAWEKVPAPSQPAIEAVLASSTTPKAKSAKWDQFADDRFIKELVSSGMFR"
     gene            complement(2187..3260)
                     /locus_tag="VFY96_16205"
     CDS             complement(2187..3260)
                     /locus_tag="VFY96_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008631537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX5608070.1"
                     /translation="MATDPNTNKRINHEKLTRFVQAAFEKLGVASRDAQIAAEALVAA
                     DLRGVDTHGVIRFSPHAWYVKWLSDGSMTAKPNIRVISETPSTALLDGDRGMGMVIGH
                     RAMELAIDKAKHCGIGMVGVRNSRHYGMSAQYAMQALAHDMIGIAMTNASRQVVPTFG
                     REARFGTNPMCFAVPADKELPFVLDMATTTAAAGKLELAARLEKPIPVGWALDEKAEA
                     TDDPRVAQKARRLLPLGGSRDNGSHKGYGLAILVEILCGVLTGTLTALNADQDPRGHF
                     FGAIRVDAFRPVSEFKKDMDRLIRELKSTPPIEGQRRVYVAGAIEFETADERAERGIP
                     LLPSVLKGLREVSEQLGLPYNLE"
     gene            3407..4435
                     /locus_tag="VFY96_16210"
     CDS             3407..4435
                     /locus_tag="VFY96_16210"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5608071.1"
                     /translation="MKKTDLFRLHWLVRVSLPVAILVVLVSPANSAELPLVRIAHGAF
                     SEKILLMWLGAEQGLFRKHGVNVEVINIRSGPQTMAALASGDIQIAYTIPGSVVSAAT
                     SGFDVAFFAGIVNKADGDFIASPQIYTPQDLRGKRVGVQSIGGGVWSMAMLALEHLGL
                     EPNRDKMTILIVGDSPVLAQSLESGGIEATYLNYVYSRSLKEKGFPVLLDLGKAPIPY
                     QGLAAATRRSFLQQNPQVIDALMRGFVESVAFIHKAQNKELAVKSLMKNLRLKNTQDG
                     EIGYQALQWLYSLDIKPTIAGIQNMARLLALGNPKVRSVKMEDVVDETPWQRLEKSSF
                     YRELATRK"
     gene            complement(4464..>5219)
                     /locus_tag="VFY96_16215"
     CDS             complement(4464..>5219)
                     /locus_tag="VFY96_16215"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5608072.1"
                     /translation="GRVQADVLGNSPEPLLYLKQQGVLAAYRSPETVNLLKGAWDPEG
                     YWSGMTTDLLVTGFNPRLLSRSAAPKNYDEYLRPQFKGQIAVNRGVPYPLTGMVSLRG
                     EEQGIAYFKKLSQQDLRLVEGYTHTVNLMAAGEYPLTAFMQVSKLDAMKRKDAPVDWL
                     PSAPTLATISTVAMVKNPLHPAAAQLLIDFYLSSEGQRGLAAAGKIPLRRGVKSPSRD
                     IDQLMESGNLHVILPEGGYDRYMKIYNEYLGAR"
BASE COUNT         1171 a         1443 c         1488 g         1117 t
ORIGIN      
        1 tgcagtacgt cagcattcct gggagccagc cgaaaattgc tgcgctgcag caaggaatta
       61 ttcagggggc attgctcgcg ccgccttccg attacgtcgc gctcaaagcc ggattcaagc
      121 gcttggtcaa tttagccgac cttttcaaag acacatcttt cagtggactc gccgcgaccg
      181 gaaaaaccgt tagggagaac ccgcaattcg tcaaacgcat ggtgcgcgcc gtggttcgcg
      241 gcgtgattca tagccgtgat tatcccgaag acgcgattca aacgatgatg aaacattggc
      301 atatggatcg cgatgtctcg gtggatgcat acaatctcgt caaggaagct ttgatccccg
      361 tgcccactga aaagggcgtc gagctgatgg cgcattggca gtcgatcgct ttgaatgtgc
      421 cgcccaaacg gccggcgcgc gattatatgg atttgcgttt tgtcaaagaa gtcatgaccg
      481 aactggaaaa gaaatgaggt gttcatgatg cggaaaattg tttttctagc ggcattgctg
      541 ctgagcgcca cgatttccaa cgcggcggaa actccggaaa agaagagccc gctttacgcg
      601 actttgaaaa ccagcatggg cgatatcgtc attcaacttt tcgaagacaa ggcgcctaaa
      661 acggtggcca atttcgtcgg cctcgctagc ggcaccaaag attggatcga tcccaagacc
      721 ggcgacaaag tgaagcggcc gctttacaac ggaacgattt tccaccgcgt cattccgggt
      781 ttcatgattc agggcggcga tcccctcggc aacggcaccg gcggcccggg ttataaattc
      841 gaagacgagt tcagtcccga gctgcggcac aacaaaccgg gcatcctttc catggcgaat
      901 gcgggaccca atactaatgg aagtcagttt ttcattaccc ataaggagac gccctttctc
      961 gatggcaaac acagcgtgtt cggcgaagtc gtcaaaggac aagaagtggt ggtggcaatc
     1021 gccaatgtgc cgcgggatgg gcgcgatcga ccgaacaaag acgtcgtgat caaagaagtc
     1081 gtcatcacgc ggggcagcta ttaacgggga attttgaggt gaaacgatga accgccgtac
     1141 gtgcggcttg gaaaaaattt gccgggcggc gcttctcgtc ctcttcgcat tgtcaggttt
     1201 gttgcggccc agcctggccg ccgagccggc gctgcttcaa accactgcag tttacggcgt
     1261 cattgccacc gatcctcttt atctgtggat cgcccaagat aaggggttgt ttcagaagaa
     1321 cggccttcag gtcgatctca cgcacattcc aaccaatcag ggggtgcagg cgctggtcgc
     1381 ggggaaagtc aatttcgtga ccgccggtcc acagatcctg gaagcaaatc tcgccgggtc
     1441 ggatacggtg tatattatga gcccggtgaa cgctttcgtc ttttctcttt atagccaacc
     1501 cgcaatcgct aatattaaag cgctggcggg aaaaaccttt ggcgcgacca acaaagggac
     1561 accgagcgac attgcggcgc acatgctgct cacgaaaaac ggcctcaagc ccgatatcga
     1621 tgtaaaattc gcttacttaa aggaaatccc ggcattggtc gcagcgctgc agcaaggcat
     1681 cattgacgct gcgttactca ccccgccagc gacgctgacg gcgcgtaatt ttggcttgaa
     1741 ggagctcttg aatgtcaccg agctcaaaat tccgttcgtg cagcatgcca tcggcacgac
     1801 tcgctcgtat atcagcgcca atccggaagt ggttcggcgc ttcgtacgaa gcgctgccga
     1861 gggactcgat tttttgcgca ggaatcgtac ggatgcggta gcgatcatgt cgaaatacac
     1921 caaaatcagc gatccggcgc agctcaacga agggctcgat gcctacgata aggcttggga
     1981 aaaagtcccc gcgccatcgc aacccgcgat tgaagcggtg ctcgcgtcat cgacaacgcc
     2041 caaagccaaa agcgccaagt gggatcaatt cgctgacgat cgctttatca aagaattggt
     2101 ttcctctgga atgttcaggt agggccgact cgtcagggcc gagctaagtg tcggccctga
     2161 gacacaggcg cagaatcccg cgagaattat tccaaattgt agggcaggcc gagctgctca
     2221 ctcacctcgc gcaatccttt gagaacggac ggcaataaag ggatgccgcg ctcggctctt
     2281 tcgtcagcgg tttcaaattc gatcgcgccg gcgacgtaga cgcggcgctg cccttcgatg
     2341 ggcggagtgg atttcaattc acggatgagc cggtccatat ctttcttgaa ctcagacaca
     2401 ggacgaaagg catcgacacg aatagcgccg aaaaaatggc cacgcggatc ttgatcggca
     2461 ttgagcgcgg tcagggtgcc ggtcaaaacg ccgcaaagaa tctctacaag aattgccagg
     2521 ccgtacccct tgtgactgcc gttgtccctc gaccctccta ggggcagcaa ccggcgcgcc
     2581 ttctgtgcga cgcgcggatc gtctgtcgct tcggctttct cgtctaacgc ccagccgaca
     2641 ggaatcggtt tttccaaccg ggcggcaagc tcgagcttgc ccgctgccgc ggtggtggtc
     2701 gccatatcca aaacaaaagg caactccttg tccgcgggaa cggcgaagca catggggttg
     2761 gtgccaaacc tcgcttcacg gccgaaagtg ggtaccactt gtcggctggc attcgtcatc
     2821 gcgataccga tcatatcgtg cgccagcgcc tgcatcgcgt actgcgccga catgccgtaa
     2881 tggcggctgt tgcgcacacc caccatgccg atgccgcagt gcttcgcttt gtcgatggcc
     2941 aactccatcg cgcgatgacc aatcaccatg cccataccgc gatcgccatc gagcaatgcc
     3001 gtcgaaggag tttcggaaat aactcggatg ttaggcttgg cggtcatgga gccgtcgctc
     3061 aaccatttca cataccaggc gtgcgggcta aaacggatca cgccgtgggt gtcgacgccg
     3121 cgaagatccg ctgccacgag cgcttcagcg gcgatctgcg catcacggct ggcaacgcct
     3181 aacttctcga aggccgcctg cacgaagcgg gtgagctttt catgattgat gcgcttgttt
     3241 gtattcgggt cggtcgccat cgtgagctcc ttgatatact ggaggcttag cgttgaagct
     3301 tgtcgcctgt caagtttatg cgcgtacgct tcgatcgact tgacggccgc aggctggttt
     3361 gcctatggtc gcgccaatcg cactcccggt tgctgaggat ccctgcatga aaaagactga
     3421 tctattccgc ttacattggc tggttcgggt aagtcttccc gtggctatac tcgtggtcct
     3481 ggtaagcccg gcaaactcag ctgagctgcc cctggtgcgc atcgcgcacg gcgcgttcag
     3541 cgagaaaatt ttgctcatgt ggctcggcgc cgagcaaggg ctgttcagaa agcatggtgt
     3601 caatgtggaa gtcatcaaca tccgaagtgg accgcaaacc atggcggcgc tggcttcggg
     3661 cgacattcaa atcgcctata cgattcccgg ttcggtggtc agcgccgcga cgagcggttt
     3721 cgacgtcgcg ttttttgccg ggatcgtcaa caaagcggac ggcgatttta tcgcgtcacc
     3781 gcaaatttac actccgcaag atttgcgcgg caagcgtgtt ggcgtacaaa gcatcggcgg
     3841 tggcgtgtgg tcgatggcga tgctcgcgct cgagcacctg ggcttggagc cgaaccggga
     3901 caagatgacg atattgattg tcggcgattc accggttctg gcgcagtctc tggaatccgg
     3961 cggtattgaa gcgacctatt tgaattatgt ttacagccgc tcgctcaagg aaaagggctt
     4021 tccggttctt ctcgatcttg gtaaggcgcc gataccctat cagggactgg cagcggcgac
     4081 ccgacgatcg tttctccagc aaaacccgca ggtcatcgat gcgctgatgc gcggttttgt
     4141 cgaaagcgtg gcgttcatac acaaagcgca gaacaaagaa ctagcggtga agtcgctgat
     4201 gaaaaatttg cggctcaaga atactcagga tggggagatc ggctaccagg cactccagtg
     4261 gctttacagc ctcgacatca agccgaccat tgcgggaatc cagaacatgg cgcggcttct
     4321 cgcgctcggc aatcccaagg tcagatcggt caaaatggaa gatgtcgtgg atgaaactcc
     4381 gtggcagcgg cttgagaaaa gctcatttta ccgggagctc gcgaccagaa aatagtgacg
     4441 cagcgcgcca ctaaagcttc aggttatctc gcgccgagat actcattata gattttcatg
     4501 tagcggtcat agccgccttc aggaaggatc acgtgtaaat tgccgctctc catcagctga
     4561 tcgatgtcgc gcgatggact ttttacgccg cggcgcagcg gaatttttcc ggccgccgct
     4621 agtccgcgtt gaccttcgct ggataaatag aagtcgatga gcaactgcgc cgccgccggg
     4681 tgcagcggat ttttgaccat ggcgacggtg gagatcgtcg ccagagtcgg cgcgctgggg
     4741 agccagtcga ccggcgcgtc cttacgcttc atggcatcaa gcttcgagac ctgcatgaac
     4801 gcggtgagcg gatactcgcc ggcagccatc agattgacag tatgggtgta gccttccacc
     4861 agacgcaaat cctgctgcga tagtttcttg aaataagcga tgccctgctc ttcgccacga
     4921 agcgaaacca tgccggtcaa aggataaggc acgccgcgat tgacggcgat ctgccctttg
     4981 aattgcgggc gcagatattc atcgtaattt ttcggcgcgg cggatcggga aagtagccgc
     5041 ggattgaacc cagtcacgag cagatctgtg gtcataccgg accaatagcc ttcgggatcc
     5101 caggcgcctt tcaagagatt tacggtttcc ggcgaccgat aggcagcgag cacgccttgc
     5161 tgtttcaaat agagcaaagg ctcgggactg tttcccaaca cgtcggcttg cacccgccc
//
LOCUS       DASULZ010000790         2667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1512542, whole genome shotgun sequence.
ACCESSION   DASULZ010000790 DASULZ010000000
VERSION     DASULZ010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512542"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="VFY96_16220"
     CDS             complement(<1..695)
                     /locus_tag="VFY96_16220"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5608073.1"
                     /translation="MNQPGEIIDGDGHIFEDGDGIARRFPFSAAGARLRSGVFPLNSH
                     IQYSLTRTPPGAFATTPDGRFKNPGPEGWIGFMDQIGIGHAVLFPTAGQRIGRIVDRD
                     YAVGAARAYNDWLAETYLQRDSRFKGIAIVPMHDAEAALTELHHAYTELGMCGVLFPA
                     TGVHLNLGAKPYWTVYAEAARLGCPIVVHGGGHWDLGMDTMNVSTGANAIGHPMSLAI
                     ALAEMVLNNLFDR"
     gene            1024..1884
                     /locus_tag="VFY96_16225"
     CDS             1024..1884
                     /locus_tag="VFY96_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX5608074.1"
                     /translation="MNHNSAQNEDVVVHLIDGTYELFRHFYGLRRFNKGEDRPYGAVI
                     GVLNTVLQMIETGASHVGVATDHVIESFRNALWPGYKTGAGIDPALLAQFHPLEDALA
                     AMGVAVWPMIELEADDALASAANIASQDDRAQKICIWTPDKDLAQCVRGDRVVQMDRR
                     SNKIRGAAGVREKFGVEPALIPDFLALVGDAADGYPGIAGIGPIGAARLLNANGTIEN
                     FPPALLGNNRELALLFKRLATLRGDASLFNSIDELRWRGPAGAFTNCVKRLSDPRLLR
                     RAMAATDRID"
     gene            complement(2014..2328)
                     /locus_tag="VFY96_16230"
     CDS             complement(2014..2328)
                     /locus_tag="VFY96_16230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608075.1"
                     /translation="MKRFLRGWIEGIRVAKTDKETTISVMQKFLKTSDRSVLETIFEV
                     YKSVHERVPTPDPKLMAVALKQLAASIPQTNPLKFEDFTDQSLIDELESEGFITKVYE
                     NR"
     gene            complement(2427..>2667)
                     /locus_tag="VFY96_16235"
     CDS             complement(2427..>2667)
                     /locus_tag="VFY96_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608076.1"
                     /translation="TRSSIRKPEELKNKKVGGDRYGSLSDLILREALRYYNLVPDRDV
                     AVIQTGGLPERLGALKPARSTARLSPVIPRWKPRS"
BASE COUNT          592 a          762 c          713 g          600 t
ORIGIN      
        1 taacgatcga aaagattgtt gagcaccatc tccgccaaag cgatcgccaa agacatcgga
       61 tgaccgatgg cattagcgcc ggtggaaaca ttcattgtat ccatacccag atcccaatgt
      121 ccgccgccgt gaaccacgat cggacagcct aacctagccg cctcggcgta aaccgtccag
      181 taaggctttg cgccaagatt caagtgtact cccgttgcgg gaaacaggac cccgcacata
      241 cccaattcgg tgtaagcatg gtgcaattcc gtcaatgccg cttccgcatc atgcatcggc
      301 acaattgcaa tgcctttgaa tcgtgagtcc cgctggagat aagtctcggc caaccaatca
      361 ttgtaggcgc gcgccgctcc aacagcgtag tcgcgatcga caattcgccc gatccgttgc
      421 ccggcggtgg gaaaaagaac cgcatgacca attccgattt ggtccatgaa tccgatccat
      481 ccctccgggc cgggattttt gaaccgccca tcgggcgttg tagcaaacgc gccaggcggc
      541 gtgcgcgtca gcgaatattg gatgtggcta ttcaatggaa acacaccgct gcgcaaacgc
      601 gcgccggcgg cgctaaacgg aaaacggcga gcgatcccat cgccgtcctc aaaaatatgg
      661 ccatcgccat cgataatttc gcctggctga ttcattcagt tcctccacgg atcaagtttg
      721 atcacgtttc agacgccaat gactcgagaa aaacggcatc cacccgaaat ctagcaattt
      781 gctctatcac ccggttacaa aaattgtcaa cgatgtcgtc tccgattgat cgttgccgac
      841 accacaccgc ttttctccag ttgacggcaa gaaagacatt tgggtacgga gaggcaactt
      901 tctggtttcg gtggatccat ggccgtggca tgaaccctcg ccgtacgcca gcaacgcatg
      961 gacacagccc tcgctggcga ctcgtccccg cacggattgc tatgctagac tacaaaactt
     1021 ccaatgaatc ataactcggc gcagaacgag gacgtcgtcg ttcatctaat cgacggcacc
     1081 tacgagttgt tccggcactt ctatggtctt cgccgcttca acaaaggcga agatcggccc
     1141 tatggtgcgg tcattggtgt gctcaatacg gttttgcaga tgatcgaaac gggcgcgtcc
     1201 cacgtcggtg tcgcgaccga tcacgtgatc gaatcctttc gcaacgcgct ttggcccggc
     1261 tacaaaaccg gcgcgggcat cgatcccgca ctgctcgcgc aattccaccc gctcgaagac
     1321 gcgctcgcgg caatgggggt ggcggtatgg ccgatgatcg agctcgaggc tgacgatgcg
     1381 cttgcctccg ccgcaaacat tgcctctcaa gatgaccgag cgcaaaaaat ctgtatctgg
     1441 acccccgata aagatttagc gcagtgtgtg cgtggagacc gagtcgtaca gatggatcgc
     1501 cgcagcaaca agatccgcgg cgccgccgga gttcgtgaga agttcggcgt ggaacccgct
     1561 ctgattccag attttcttgc cttggttggc gatgccgcgg atggctatcc gggcatcgcg
     1621 ggaatcggtc cgatcggcgc ggcacggctc ctgaacgcaa acggcacgat cgagaatttt
     1681 ccgcccgcct tactgggaaa caaccgggag cttgcgttgc ttttcaagcg cctcgcgacg
     1741 cttagaggcg acgcgtcgct cttcaattcc atcgacgagc ttcgatggcg cggacctgcc
     1801 ggcgcattta cgaactgtgt aaagagactg agcgatcccc gtctactgcg ccgcgctatg
     1861 gcggcaacag acagaattga ttgacacttt ttgttcctaa gcggttcgtg ccgcaaaaga
     1921 tttctcccgg agtttatcct gagtttctcg aaggagtcga aataacagca gcgcttaaat
     1981 tcaggcatcg agacattccg tccattggcg aaatcagcgg ttttcgtaga ctttggtgat
     2041 aaatccttcg ctctcgagct cgtcgatcaa gctttgatcg gtgaagtctt cgaacttgag
     2101 cggattcgtt tgcgggatgc tggcggcgag ttgtttgaga gcgacggcca ttaacttggg
     2161 atccggtgtc ggaactcttt cgtgaacaga tttgtaaact tcaaaaatcg tttcgagaac
     2221 ggagcggtcg ctggtcttga gaaatttctg catgaccgaa atggtggttt ctttgtcggt
     2281 tttggcgact ctgatgccct cgatccagcc gcgaaggaat cttttcaccg tatctcgctt
     2341 cgccgccaaa aacgatttgc tgacgatgat ggtgctgctt ggatagcgga tcggcagctg
     2401 cgagagatcg atcagtttgt gaaaacctaa cttctcggct tccagcgcgg tatcaccggt
     2461 gacaatcgcg ccgtcgaccg cgccggcttt agcgctccga gccgttcagg caacccgccg
     2521 gtttgaatga ccgccacatc ccgatccggc actagattgt aatagcgtag cgcctcgcgc
     2581 aggatcagat ccgaaagcga gccgtagcga tcgcccccta cctttttatt tttgagctct
     2641 tcgggctttc taatcgagct gcgagtc
//
LOCUS       DASULZ010000791         3678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_93033, whole genome shotgun sequence.
ACCESSION   DASULZ010000791 DASULZ010000000
VERSION     DASULZ010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93033"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1224)
                     /locus_tag="VFY96_16240"
     CDS             complement(91..1224)
                     /locus_tag="VFY96_16240"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5608077.1"
                     /translation="MALPRAVKELDEESKAETEEKPNIRELATVLEPFNVRSLALTGL
                     LILAVFFTLKVASSFFIPVVLAILLNFVFGPTIRAMARVWIPPPLGAFIVLGCLIGGV
                     AFGIYQLSGPARAWLAKMPETAQQIERKFSDLKRSMREVTKAGQEVDRLTNLGNEKTQ
                     KVEVRRPSFGETLLAPTQDFIVGIGTVLILLFFLLASGDLFLRKLVTVLPRFEDKKTA
                     VEISRQIERDISTYLFSICLINAFFGTAIGTAMYFLGMPNPLLWGVMAGLLHFIPFLG
                     AVVGISIVTLVAAMTLDTIGSILLVPASYLGLNLLEEYLCVPLVIGRRLMLNPVVLFI
                     WLIFWGWLWSVPGALMAVPLLAIVKIVCDHIKPLAAIAEFIGD"
     gene            1516..2121
                     /locus_tag="VFY96_16245"
     CDS             1516..2121
                     /locus_tag="VFY96_16245"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX5608078.1"
                     /translation="MAITLYHDTPSSNCDRVKIALAEKNLNWDGVRIGLANKEQKRPE
                     FLELNPYGKIPVLVEDGKVLFESCIINEYLDEKYPNPSLMPKDPYLRGRGRILVDYTL
                     NYIHEPYWSLRGEMLKPQVERNSSVLEQTRGRLRELLQYLETALDEKDYFLGALSLTD
                     IDVLPRFLRMESYGALPEPGLPRLSAWLQRMKERPSVQSIL"
     gene            complement(2283..3320)
                     /locus_tag="VFY96_16250"
     CDS             complement(2283..3320)
                     /locus_tag="VFY96_16250"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5608079.1"
                     /translation="MRRSYLIVMGLVLIVIFTMATDIRAAAVGEDFYRGKVIRIVVGF
                     SAGGGFDTYARTLSRSMGKYIPGHPTVIVENMTGAGSLIAANHIYRVAKPDGLTIGAF
                     NGNLILARLSGAQGVEFDPIKMSWLGAPGYNHDLCVLNRKTAITSAEQWLALKTPLKL
                     GGSAPGSTTDDTAKVLKEAIGLPMRLITGYKGTADIRVAVESGELDGLCGFSWVSVRS
                     TWRKAIEAGDVIIVLQSAPKPHPELPNVPLSINFAKSEEAKQLMEAGIHAPSAMTYGY
                     SLPPNTPKDRIQTLRAAFLQTVKDSDFLNDSGKANLEVAPASGEEIEQSIQKLFKTPS
                     PVVAKLKEVLK"
     gene            3484..>3678
                     /locus_tag="VFY96_16255"
     CDS             3484..>3678
                     /locus_tag="VFY96_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608080.1"
                     /translation="MRLKYLPLSMLFLLAFALNVHPAEFYQGKTIRVIVGGSAGGGFD
                     IYTRAMARHMGKHIPGSPTLI"
BASE COUNT          884 a         1008 c          963 g          823 t
ORIGIN      
        1 aagttatcac gacgcgcgtt ttgttagcgg ctggactgat cgtcggttct gtagctctgg
       61 tgagtggccg taaaacctga ccgagcgcaa tcaatcgccg atgaactctg cgattgccgc
      121 gagtggcttt atatgatcgc agacgatttt cacaatcgcc aacaaaggaa cggccattag
      181 cgctcccggt acactccata gccagcccca aaagatcagc catatgaaaa ggacgaccgg
      241 attgagcatg agccggcggc cgatgacgag aggaacacat aagtactcct cgagcaggtt
      301 caagccgaga tagctagccg gcactagcag aatggagccg atcgtgtcga gcgtcattgc
      361 cgctaccaaa gtgacgatgc tgatgccgac cacggctccg aggaacggaa taaagtggag
      421 cagccccgcc atcacacccc ataacaaggg attcggcatt ccgagaaagt acatcgcggt
      481 tccgatcgcc gtgccgaaaa acgcgttgat caggcatatc gaaaatagat acgtcgaaat
      541 atcccgctcg atttgccggc tgatttccac cgcggttttc ttatcttcaa aacgcggcaa
      601 gaccgtcacc agcttgcgca aaaacagatc gcccgaggct aagagaaaaa aaagcagaat
      661 taaaaccgtg ccgatgccga cgataaagtc ctgagtgggc gccagcaaag tttcgccaaa
      721 acttggcctg cgcacttcga ctttttgggt tttctcatta cccaagttcg tcagccggtc
      781 gacttcctgc cccgctttgg taacttcgcg catcgacctt ttcaagtcac taaacttacg
      841 ctcgatctgt tgcgcggttt ccggcatttt cgccagccag gcgcgagcgg gtcccgaaag
      901 ctggtaaatc ccgaatgcga caccaccgat caagcaacca agaacgataa aagcgccaag
      961 cggcggcgga atccatacgc gtgccatcgc ccggatagtt gggccgaaaa caaaattgag
     1021 caagatggcg agaacgacag ggataaagaa cgagctcgcc accttcaatg tgaaaaaaac
     1081 cgccaaaata agaaggccgg tcaatgcgag cgaccgcacg ttgaacggct cgagaaccgt
     1141 agctagctcg cggatattcg gtttctcttc ggtttccgct ttagattctt cgtccaactc
     1201 tttaacagct cgcggcaatg ccatgatgta acctcggcgc ccggagctga aattcggttg
     1261 gagaccgggc ggctctatta agaataacct gctatggggt ggttttgaac tggcagaaaa
     1321 aacaactttt tttcaatggc taaagctcgc ccggttttaa gcagcatgcg tttcgccttt
     1381 cggggcgcta acgattggac ggtgaaaatc gcctcttttt gcacattcga gcgatacaac
     1441 cttgtcgctc ttccgctgtc cgaatcatat tgcgacacac ctggagcgat gttaaaggaa
     1501 gaattcggag ggcctatggc aatcaccctg tatcatgaca cgccgtcatc caactgcgac
     1561 cgggtaaaaa ttgccttggc tgaaaaaaat ctaaactggg acggcgtgag aatcgggctc
     1621 gctaacaaag agcagaaacg accggagttc ctggagctca atccgtacgg caaaattccg
     1681 gttctggtcg aggacggcaa agtcttattc gaatcctgta tcatcaacga atatctcgac
     1741 gaaaaatatc cgaacccgtc gctgatgccg aaagaccctt acttgcgcgg gcgcggacgc
     1801 attctcgtgg actatacctt gaactacatt cacgagccgt attggtcgct gcgtggcgaa
     1861 atgctgaaac cacaagtaga gcggaactcc tctgttcttg agcaaacgcg tggacgtctg
     1921 agagaactgc ttcaatactt ggaaacggcg ctggacgaaa aggactattt tctcggcgcg
     1981 ctaagtctca ctgatattga cgttctgccg cggtttctgc ggatggaatc ctatggcgcg
     2041 ctgccggagc ccggcctgcc gcggctcagc gcctggcttc agcgcatgaa ggagcgcccg
     2101 tcggtccaat cgattctttg agtaaaagta ctagggtttt tttttcatgc cgatgaaaac
     2161 cggtgacaac tgcctaaaac tcctcctgca tccagatcgc gcgaccccgg actagccggg
     2221 ggtcgcccgg aatgagatct ttttttgcgt acttccagaa cagtagtgtt agcgatcagc
     2281 gctcacttca gcacttcttt caatttggcg accacgggcg aaggcgtctt gaatagcttc
     2341 tgaatcgact gctcgatctc ttcgcccgag gcgggcgcca cttccaaatt cgctttccca
     2401 gagtcgttca aaaagtcgga gtctttcact gtttgtaaaa atgccgcgcg aagtgtttgg
     2461 atccgatcct tcggtgtatt gggcggtagc gagtaaccat atgtcatcgc gctcggcgcg
     2521 tgaattccgg cctccatcaa ctgtttagcc tcttccgatt ttgcaaagtt aatcgacagc
     2581 ggaacgttgg gcagctcggg atggggcttc ggcgcgcttt gcaggacgat tatgacgtct
     2641 cccgcttcga tcgccttgcg ccaagtggag cgcaccgaga cccacgaaaa cccgcacaat
     2701 ccgtcgagtt caccggattc gacggcgacg cggatatccg ctgtgccttt gtagccggta
     2761 atcagccgca tcgggagtcc gatcgcctcc ttcaatacct tggcggtgtc gtcggtcgtg
     2821 gagccgggcg cgctgccgcc caatttcaac ggagttttca acgccagcca ttgctcggcg
     2881 ctcgtgatgg cggttttccg attgagcacg cagagatcgt gattgtagcc aggcgcgccc
     2941 aaccagctca tctttatcgg gtcaaattca actccctggg caccgctgag acgcgcgaga
     3001 atgagattgc cgttgaacgc gccgatggtc agcccgtcag gcttggcgac gcgataaatg
     3061 tgattggcag cgatgagact cccggcgccg gtcatgtttt ccacgatcac cgtcggatga
     3121 ccagggatat actttcccat tgagcgggac agcgtgcgcg cgtaggtatc aaaaccgccg
     3181 cccgcggaaa aaccaacaac gatacgaatc acttttccac gataaaaatc ttctccgacg
     3241 gcggcggcac gaatgtccgt tgccattgtg aagatcacga tcagcaccaa tcccatgaca
     3301 atcaggtagc ttcgtctcat ggcttattcc tcttcttgaa taacgtcgcg cgcacaagct
     3361 atgcgaaacc gtcgaatttc gtcaaccata aaatagctaa agatgccgat gtcgcacggc
     3421 ttcgaatcgc gttgacaatc ggacctgttt gtcgatattg acccagttcg gggggaaaat
     3481 cggatgcgtc ttaaatatct gccactgagt atgctctttt tgcttgcatt cgcactgaat
     3541 gttcatcctg ccgagtttta tcagggtaaa accattcgag tcatcgtcgg cggcagcgcg
     3601 ggcggcggct tcgatattta tacccgcgcc atggcgcgcc acatgggcaa gcatattcca
     3661 ggaagcccga cactgatt
//
LOCUS       DASULZ010000792         3627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1209040, whole genome shotgun sequence.
ACCESSION   DASULZ010000792 DASULZ010000000
VERSION     DASULZ010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209040"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VFY96_16260"
     CDS             <1..411
                     /locus_tag="VFY96_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006787978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5608081.1"
                     /translation="TGKWMRGMSERLETWLHMRQPEADLTKLEERMDPALAEKIYELD
                     RCIECGCCVAACGTARMREDFVGAVGLNKIARFRLDPRERRNDDDYFELIGDDDGVFG
                     CMSLMACHDVCPKDLPLATQIAFLRRKMVAVGWK"
     gene            463..1974
                     /locus_tag="VFY96_16265"
     CDS             463..1974
                     /locus_tag="VFY96_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008959309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEX5608082.1"
                     /translation="MSKVETAPVVPTVSAKKESSSKITWKHFAPVLVALIVALIPAPA
                     GLPQYAWYYFAIFAGVIVGLMFEPLPGGAIGLIAVTLVARLSEYVLYSPEQLAKPGFN
                     PLNASLSWALSGFSNSTVWLIFGAFMFALGYEKTGLGQRIALMLVKAMGRKTLTLGYA
                     VVIADTILAPFTPSSTARSGGTIYPVIKNLPPLYDSKPDDPSMRRIGSYIMWVGIAAV
                     CVTSSMFLTALAPNLLAVELVQKIAKVELTWTDWFIYFAPVGILLLALLPLLVYWIYP
                     PQVKEGTEVQSWAAKELEKMGGFTQREITLAVLVLIALVMWIFRGDDVNATTAALIII
                     SLMLLTNVVTWSDIIKNSAAWNTLVWFATLVALADGLTRVGFVKWFAQSVAGHLSGVA
                     PSVAVAVLLLINFFGHYLFASVTAHVTAMIPVLLAVASTIPGVDMHTLALGLCLQLGI
                     MGILTPYASGPSPVYYGSGYLPSADYWRLGAIFGVIFFLAFLVIGVPWMLLIR"
     gene            2168..2893
                     /locus_tag="VFY96_16270"
     CDS             2168..2893
                     /locus_tag="VFY96_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608083.1"
                     /translation="MTQAPFVSKFLRTILVFAASLLVGCTPLNLSDIHTTSTPRSQLF
                     DVAELAREPVATLGVVSPASLQGFNPPLSYALNAALSEASPPIRGIAAHETVNMLNQQ
                     GLAGEYGTLIAGFGRAGILEREPLQRIGSALSSRYVLLPGLAEFNQVIIDRLSISGWN
                     FIQSRVTTLRLWLQLWETQTGKILWESAGEVTVATKLLRSEQTVALDDIAQKLWLRMI
                     QDNLLDGESRSRFFLSNKSKETR"
     gene            2896..3549
                     /locus_tag="VFY96_16275"
     CDS             2896..3549
                     /locus_tag="VFY96_16275"
                     /inference="COORDINATES: protein motif:HMM:NF016704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5608084.1"
                     /translation="MHATDYLVHFSNILMLAAYSVRDILWLRWFAVAAALTNIPYFLL
                     QGTVLWPPVLWALVFTAINLYQIARLYLERRPVVLSHDEQKLYDLGFRSLRPREFVSL
                     SLAGEWKNAEPGERIITEGEPVSCLSIPITGSADVHRRGERIGALTPGHIIGTGLALT
                     GEPSPVEVTFTEPARYMRWSLPSLRRFMDTRPDLRVTLQGLVNRDLSEKLNLLVKET"
BASE COUNT          708 a         1036 c         1060 g          823 t
ORIGIN      
        1 accggcaaat ggatgcgcgg catgagcgag cggctggaga cctggctgca tatgagacaa
       61 ccggaagccg atctgaccaa gctggaagaa aggatggatc cggctctggc ggagaagatc
      121 tacgagcttg accgctgcat cgagtgcggc tgctgcgtgg ccgcctgcgg caccgcgcgt
      181 atgcgcgagg atttcgtcgg cgctgtggga ctcaacaaaa tcgcgcgctt ccgtctcgat
      241 ccccgcgagc ggcgcaacga tgacgattat ttcgagttga ttggtgatga cgacggcgtt
      301 tttggctgca tgtcactcat ggcctgccac gacgtctgcc cgaaggacct gccactggcg
      361 acgcagatcg cgtttttgcg acgaaaaatg gtggccgtgg gatggaaata atgtgcgatt
      421 cagcccggca acgtcctccg caggcgcgga aaggagttcc ttatgagtaa ggtcgaaacg
      481 gcgccggtcg tacccaccgt cagcgccaaa aaagagagtt ctagcaaaat cacctggaaa
      541 catttcgccc ccgtgcttgt ggcgctcatc gttgcgctca tccccgcacc ggcagggttg
      601 ccgcagtacg cgtggtatta cttcgccatt tttgccggcg tgatcgtcgg attgatgttc
      661 gaacctttgc ccggtggtgc catagggttg atcgccgtga ctttggtcgc acgattatcc
      721 gagtacgtgc tgtactcgcc ggagcaactc gcgaagccgg gttttaaccc actcaacgcc
      781 tctttgagct gggcgctctc tggcttctct aatagcacgg tttggctgat cttcggcgcc
      841 ttcatgtttg ctcttggtta tgaaaagacc gggctcggcc agcgcatcgc gctgatgctg
      901 gtgaaggcaa tggggcgaaa gacgctgact ctcggctatg cagtggtgat tgccgatacg
      961 attctggcgc cgttcactcc ttcaagtacg gcgcgcagcg gcggcaccat ttacccggtg
     1021 attaaaaatt tgccacccct ctacgactcg aagcccgacg acccgtccat gcgccggatc
     1081 ggctcctaca tcatgtgggt gggaatcgcg gccgtctgtg tgacaagctc aatgttcctc
     1141 accgcgcttg cgcccaacct gctcgctgtc gagctggtgc agaaaatcgc caaggtcgag
     1201 ctcacgtgga cggactggtt catctatttc gctcccgtcg gcattctttt gctcgcccta
     1261 ttgccgctgt tggtttactg gatctatccg ccgcaggtga aggagggaac ggaggtccag
     1321 agctgggcgg ccaaggagct ggagaaaatg ggagggttca cccagcgcga gattaccctg
     1381 gcagtcctgg tcctgatcgc tctagtgatg tggatctttc gaggtgatga cgtgaacgcc
     1441 accaccgctg cactcatcat catttcattg atgctgctga ccaacgtggt gacctggagc
     1501 gacattataa aaaactctgc agcctggaac acgcttgtgt ggttcgcgac tctggtcgca
     1561 ctcgcggacg gcttgacccg cgtgggtttc gtgaagtggt tcgcgcaaag tgtcgccggt
     1621 catcttagcg gcgttgcacc tagcgttgcg gtggccgtgc tgctgctgat taattttttc
     1681 ggccactatc tgttcgcgag cgtcaccgcc catgtcaccg ctatgattcc ggtgctgctc
     1741 gcggtagcgt cgacgattcc gggagtcgac atgcatacgc tcgcactcgg cctgtgcttg
     1801 cagctcggca taatgggcat tctcactcct tacgcgagcg ggcccagccc cgtgtactac
     1861 ggtagcggtt atctgccgtc ggccgactac tggcggctgg gcgcgatttt cggcgtaatc
     1921 tttttcctgg cgttcctggt tatcggcgtg ccttggatgc tgctgatacg gtgagcttct
     1981 tcgagcaaac aatgtttgct gggctgcaaa agcctggatt gctacgccat gaagccgatc
     2041 gtcgagatac tcgacgcggt gataaacggc agagtagtag gcagaatcgt cggggatctt
     2101 ggttctaaca tcatggcgcg atgacgcatc tcggtgccgt tgcctccgat ttattgagga
     2161 ggtcattatg acgcaagctc ccttcgtcag taaatttctc cgaacaatcc tggtattcgc
     2221 agcatcgtta ctcgtcggct gcacaccatt gaatttgtcg gatatacaca ccacgtctac
     2281 acccaggtca cagcttttcg atgtcgccga gctcgcgcgc gagccggtgg cgacgcttgg
     2341 tgtcgtcagc ccggctagtc tccaagggtt caacccgcca ttgtcttatg ccctcaacgc
     2401 ggcgctctcg gaagcctccc cgccgattcg cggaatcgcg gcccatgaga cagtcaacat
     2461 gctcaatcag caagggctgg ctggggagta tggcactctg atcgcgggct tcggtcgcgc
     2521 cggcattcta gagcgcgagc cgttacaacg gatcggatcg gccctctcct ctcgctatgt
     2581 gctgctaccc gggctcgcgg agttcaatca agtcataata gatcggcttt cgatctcagg
     2641 ctggaacttt attcagagcc gggtcactac cctgaggcta tggctgcagc tctgggaaac
     2701 acagactgga aagattctct gggagtcagc tggggaagtc accgtcgcga ccaagctcct
     2761 caggtcggag cagaccgttg cccttgacga catcgcgcaa aagctgtggc tgcgcatgat
     2821 ccaggacaac ttgctggatg gggaaagcag atcacgattt ttcttgagca ataaaagcaa
     2881 ggagacaaga taaatatgca cgctacagat tatctggtgc atttctcgaa catcctgatg
     2941 ctagcggcgt attcggtgcg cgacatcctg tggctgcgct ggtttgccgt tgccgctgcg
     3001 ctcaccaaca tcccgtactt cctccttcag ggtaccgtgc tctggccacc ggttctctgg
     3061 gcactcgtat tcacggccat caatctttat cagatcgcgc gcctctacct cgagcgacgt
     3121 ccggtagtgc tctcccacga cgagcaaaag ctctatgatc tgggattccg ctcgcttcgc
     3181 ccccgcgagt tcgtttcact gtcgctcgcg ggtgaatgga agaatgccga gcccggcgag
     3241 agaatcataa ccgaagggga gccggtgtcg tgcctgagca ttccgatcac tggaagcgca
     3301 gacgtccaca ggcgtggcga gcgcatcggg gcgctgacac cgggccacat catcggcact
     3361 gggctggccc tcaccggtga accatcaccg gtggaagtaa ccttcaccga gcccgcacgt
     3421 tacatgcgct ggtcgcttcc aagcctgcgt aggttcatgg acacgcggcc cgacctgcgc
     3481 gtgacgctgc aaggtctcgt gaaccgcgac ctctccgaaa aactgaatct tttagtgaaa
     3541 gagacctaac ggtgcgcaac aagcacgtaa ataaggcgag gtgattagtt gatcggagtg
     3601 ccttggatgc tggcgattcg gtaaaca
//
LOCUS       DASULZ010000793         2247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1209059, whole genome shotgun sequence.
ACCESSION   DASULZ010000793 DASULZ010000000
VERSION     DASULZ010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209059"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFY96_16280"
     CDS             complement(<1..234)
                     /locus_tag="VFY96_16280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608085.1"
                     /translation="MTSKLARITLKIGARNSFAQKTLDWLRLLIVLAMVLSLAFASWA
                     PNGLVLPVYYIVSLLLVIALPRSRDRVLVAALCT"
     gene            494..2089
                     /locus_tag="VFY96_16285"
     CDS             494..2089
                     /locus_tag="VFY96_16285"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX5608086.1"
                     /translation="MAENAYYRDFRAHLQALDDRGKLIRITREINKDTELMPLVRWQF
                     RGLEEAERKAFIFEKVIDSRGRRYAMPVTVGTLAATTEIYAIGMMCDPAEIHERWARA
                     QLHPLEPVTVSRAPAQEVIWQGKDLLNGHGLDMIPVPISTPGFDNAPYLTSANWITKD
                     FETGIYNIGNYRSQIKAPNRTGGFFLSQHMGQHWRKCKTKGKRLETCIAIGVVPSIAY
                     SATAKIPYDFDEYRLAGGLAGAPVEVVQAKTVDLLVPATAEIIIEGTIPTDVVEPEGP
                     FGEYPGYMGHRTVSPFLEVTCITHRRDAIYTALMSQFPPSESSKIKHTGTEKIIYKFL
                     RHDSGNSTVIDVALHDEVSGSGQAYCVIKMRNATKAEAWRALNATAGYTGSYAKVCVA
                     VDEDIDIHDPAMINWAICYNMRPDEDVAVVKGKMPGLDPSTYPPGAEQHDRRSAFTSA
                     LLLNAVRPWPYTPVSLPKKEFMERSKQIWEELELPPLKPRMPWYGYSLGAWSKEDEQE
                     ADSAVKGDYFATGEKQVHQRQKT"
BASE COUNT          554 a          626 c          617 g          450 t
ORIGIN      
        1 ggtgcacagt gctgcaacca gcacccgatc ccgacttcgc ggcagggcga tgaccagaag
       61 aagactgaca atgtaataaa ccggcagaac caacccattc ggtgcccacg acgcaaacgc
      121 caaactcaga accatggcta agacgattaa cagccgcagc caatcgagag ttttctgcgc
      181 aaagctattt cgcgcgccaa tcttcaaggt gattcgagcg agtttgctgg tcatttgttt
      241 tgccgtatcc gggagttcat tcacgaaaat cgaatggcca gtgtagcaaa cgtgctcgca
      301 agagcacaag aataaacccg ctgtcggcgt tccgcccacg gcgcgagctt ctcgggcatc
      361 acctatcggc agtttctacc aatcgtttcc cgtttcggaa ctacttgtcc tgcgccaatc
      421 tggatcagct ttgcttgaca agctgtgagg cacggttgat agatggcaga aacatcatcc
      481 aagggagcac ggcatggcgg aaaatgcata ttaccgagat tttcgcgcgc atctgcaggc
      541 gcttgacgat cggggcaagc tgattcgtat cacccgagaa atcaacaaag acaccgaact
      601 gatgcccctc gtacgctggc aattccgcgg cctggaggaa gccgagcgaa aggcctttat
      661 tttcgaaaaa gtgatcgaca gcagaggccg gcgctacgcc atgcccgtga cggtaggtac
      721 attagccgcg accaccgaaa tttacgccat tggaatgatg tgcgatcccg cagagatcca
      781 tgagcgctgg gcgcgcgcgc aattgcatcc gctcgagcca gtaactgtca gtcgcgcgcc
      841 ggctcaggaa gttatctggc agggaaaaga cttactcaac ggccatggcc tcgatatgat
      901 tccggtgccc atttccactc cagggtttga taacgcgccc tatctgacgt ccgccaactg
      961 gatcaccaag gattttgaaa ccggaattta caacattggc aactaccgca gccagatcaa
     1021 agcgccgaat cgcacgggcg gtttctttct ctcgcagcat atgggacagc actggcgaaa
     1081 gtgcaaaacc aaaggcaagc gcctggagac gtgtatcgct attggcgtgg tgccgagcat
     1141 cgcgtactcc gccaccgcga aaatccctta tgactttgac gaataccgtt tggccggcgg
     1201 cttggcggga gcgccagtcg aggtggtcca agcaaaaacg gtcgatctac tcgtgccggc
     1261 aaccgccgaa attattattg agggcacgat tcccactgat gtcgtcgagc cggaaggccc
     1321 gtttggcgaa taccctggct atatggggca tcgcaccgtc tcgccgttcc tggaagtgac
     1381 ctgcatcacg caccgccgcg atgcgatcta tacggcgctg atgagccagt ttccaccaag
     1441 tgaatcgagc aaaatcaagc acacgggaac agaaaaaatc atctataaat ttctccgcca
     1501 tgacagcggc aattccacgg tgatcgatgt cgccctgcat gacgaggtaa gcggcagcgg
     1561 ccaagcctat tgcgtgatca agatgcgcaa cgccaccaag gccgaagcct ggcgcgcgct
     1621 caacgccacg gcgggttaca ccggcagcta tgcgaaagtg tgcgtcgccg tggacgagga
     1681 catcgatatt cacgatccgg cgatgattaa ctgggcgatc tgttacaaca tgcggccgga
     1741 tgaagatgtc gcggtggtca aaggcaaaat gcccggactc gatccatcga cgtatccgcc
     1801 gggggccgaa caacacgatc gcagatctgc gttcacgtca gcgctgctgc ttaacgcggt
     1861 gcgcccttgg ccttacacac cggtttcttt gccaaaaaaa gagttcatgg agcgctccaa
     1921 gcagatttgg gaagagctcg agctgccgcc tctcaaaccg cggatgccat ggtacggcta
     1981 cagtctcggc gcatggagta aagaagatga gcaggaagcg gattcggccg tcaaaggcga
     2041 ctattttgcg acgggcgaaa aacaagttca ccaacgacag aagacctgac ggactccgtt
     2101 cggttcgggc gaaagccttt gtttgtggag atcgaaagtt gggggcggtt aatgctgcgg
     2161 tcagaaaatt ccgggtctcc agcgcttcac gcgtttcaaa tagttctcac atggcgtccc
     2221 aatcacttgc agcaattttt ttcatcg
//
LOCUS       DASULZ010000794         3026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_701677, whole genome shotgun sequence.
ACCESSION   DASULZ010000794 DASULZ010000000
VERSION     DASULZ010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701677"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VFY96_16290"
     CDS             complement(<1..396)
                     /locus_tag="VFY96_16290"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5608087.1"
                     /translation="MPGALDDIRVIDFTHALNGPFCTMLLGHMGAEIIKIEPPGGDGF
                     RRSWMPPDAKVDAYEFLWINVNKKSVVLNLKTARGVELARQLIAKADVVVENYHAGQM
                     EKFGLGYDDLRKLNPRLIYSCSRGFGESGP"
     gene            complement(459..1544)
                     /locus_tag="VFY96_16295"
     CDS             complement(459..1544)
                     /locus_tag="VFY96_16295"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5608088.1"
                     /translation="MPIVDADTHVDETEATWEYLREHERAFKPVTQYPSRVDPGKTPS
                     RYWLIDGHRQMRFVRDDKTTRTTVETRELLDVQARLRDMDAMGTEVQIIYPTLFLMEA
                     TERPEVSVALRRSYNRWLADRCTESHGRLRWVCLPPLMNIEDSLDELRFAKDHGACGI
                     LKKGDLEAGKWPADPYFFPLYDEANRLDLPICIHIGSGTPDLVPAREFSLGSFMRSQL
                     PAVHAFHTIIRHQLPQKFPKLRFGIIEAGCSWLPFIAWELKRRMEKAADGAGTFSQFA
                     RYTLPSELLKSNRMYVTCQVDEDLPYILKVAGEDSLMIGSDYTHRDSSMEYEFPRLLK
                     QRVNRGEISQSAVQKILYDNAKTFYGL"
     gene            complement(1876..>3026)
                     /locus_tag="VFY96_16300"
     CDS             complement(1876..>3026)
                     /locus_tag="VFY96_16300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HEX5608089.1"
                     /translation="TKTVCIEINAKGDVIRSRFFNSVIRSHERMTYTDVRRILVDHDA
                     ECLARYSALVDEFKRMEELALLLYERRRARGNLDFDLPEAEIILDLQGLPENIVRAER
                     NIAHRMIEEFMIAANEAVARHLTEKDFPTLYRVHEGPDQDALETLAPFLLSLGYRLPQ
                     KKETIGPLEIQRLLESARGKPEERVVNRVLLRSMKQAIYQPENIGHFGLASKCYTHFT
                     SPIRRYPDLIVHRILDRVMNDEKLKPNAREDLLRYLQQAGEHTSERERHAMDAEREMV
                     DLKKAQFMQNRIGEKFSGFINSLANFGFFVELDNYFIEGLVKLSSLTDDDYDYYEKEY
                     VIKGRRHGQKFRLGDNVRVRVARINAFRSEIDFELLPSNGKFVDSEPK"
BASE COUNT          660 a          795 c          852 g          719 t
ORIGIN      
        1 cggtcccgat tcgccgaatc cgcgcgagca ggaatagatc aggcgcggat ttaatttcct
       61 tagatcgtcg tagcccagac cgaatttttc catctgcccg gcatggtagt tctcgaccac
      121 gacatcggcc tttgcgatca attgccgcgc cagctcgacc ccgcgcgcag ttttgagatt
      181 gagcacgaca ctttttttat tgacgttgat ccataagaat tcgtaggcgt ccactttggc
      241 gtcgggcggc atccatgagc ggcgaaaacc gtcgccgccg ggcggctcga tcttgatgat
      301 ttccgccccc atgtggccga gcagcatcgt gcagaaggga ccgtttaacg cgtgggtaaa
      361 atcgattaca cgaatatcat cgagagcgcc cggcatattg cctcctggtc tcatgtaaat
      421 tggctgtcat cgaaggatgc gcttgcatga cgaacgcttt agagtccgta aaaagttttt
      481 gcgttgtcat aaagaatttt ttgcactgcg gactgagaaa tctctcctct gttgacacgt
      541 tgtttgagca gccggggaaa ttcatactcc atggacgaat ccctgtgggt gtagtcggag
      601 ccgatcatca aactatcttc accggcaact ttcaggatgt aaggcaagtc ttcgtcgacc
      661 tggcaggtga catacatgcg gttcgacttg agcaactctg atggcaacgt gtaacgggcg
      721 aactggctga aagtgccggc gccgtcggcg gccttttcca tgcgtcgttt gagctcccag
      781 gcgataaaag ggagccaaga gcagcccgcc tcaatgatgc cgaagcggag cttgggaaat
      841 ttttgcggca gttgatggcg aataatggtg tgaaacgcat gaaccgccgg aagctgagag
      901 cgcatgaagg atcccaagga gaattcccgc gcagggacca agtccggcgt gccggaaccg
      961 atgtggatac agatcggcag gtccagacgg ttggcctcgt cgtagagcgg gaaaaaatag
     1021 ggatccgcgg gccacttgcc cgcctccaag tcacctttct tgagaatgcc gcaggcaccg
     1081 tgatctttgg cgaagcgcaa ttcgtcgagg gagtcttcga tattcatcag tggcggcaag
     1141 cacacccaac gtaagcggcc gtgcgactcg gtacagcgat cggcgagcca gcgattgtag
     1201 ctgcgtcgca gcgcgacaga aacttccgga cgctcggtgg cttccatcaa aaaaagcgtc
     1261 gggtagatga tctgcacttc tgtacccatt gcgtccatat cgcgcagccg agcttgcaca
     1321 tccagtaatt cacgggtttc caccgtggtg cgcgtcgtct tgtcgtctcg aacaaagcgc
     1381 atctggcgat ggccgtcgat caaccaatag cggctcggag tttttccggg atcgactcgg
     1441 ctggggtact gagtcaccgg cttgaaggct cgttcgtgtt cgcgcaggta ctcccatgtg
     1501 gcttccgtct cgtcaacgtg cgtgtcggca tcgacaatcg gcatatgcac ctcacagcag
     1561 gtggctgtgc agatattaat cacaaattgc ccggcgcggt aaagtcggga acgggtcact
     1621 ttggagaagg atctttcact ttgttcgaga tgagcgctgc gaatttagcg tgatttcgag
     1681 caagggttct tcacgatgag tgattgtaac gtgaccggcg gtccacccac actaatgaaa
     1741 actcgatctg ctgagttccc acctacgttt ccgatgagtt cgtcattccc gcatgctttt
     1801 agcgggaatc caggcgatcc ggactggagc cccgattaat acattcgggg gtgacggttt
     1861 tgcagtcgtt ttgaatcact tcggctcaga atcaacaaac ttgccgttac ttggcaacaa
     1921 ttcgaaatcg atctcgctgc gaaacgcgtt gatgcgcgcg actcttacac gcacattgtc
     1981 cccaaggcgg aatttttggc cgtggcggcg gcctttgatc acgtattctt tctcgtagta
     2041 gtcatagtcg tcgtcagtca gtgaagaaag tttcaccaga ccttcgataa aatagttgtc
     2101 gagctcgacg aaaaaaccga aattggccag gctattgata aagccactaa atttctcgcc
     2161 gatgcgattc tgcatgaact gagctttttt gagatcgacc atctcccgtt cggcatccat
     2221 ggcgtggcgc tcgcgctcgg aagtgtgttc gccggcttgt tgcaggtagc ggagcaaatc
     2281 ttcgcgcgcg ttcggtttga gtttctcatc gttcatgact ctgtccagga tgcggtggac
     2341 gatcaaatcg ggataacgcc ggatcggcga agtgaaatga gtgtagcatt tggacgcaag
     2401 accgaagtgg ccgatgttct cgggctggta aatcgcctgc ttcatggaac ggagcagcac
     2461 gcgattgacg actcgctctt ccggttttcc gcgcgccgat tcgagcaatc tttgaatctc
     2521 aagcgggccg atggtttctt ttttttgcgg cagacgatag ccgaggctta gaagaaaagg
     2581 cgccagagtt tcgagggcat cctgatcggg gccttcgtgt acccggtaga gcgtgggaaa
     2641 atccttttcc gtgagatggc gcgccaccgc ttcattggcg gcgatcataa actcttcgat
     2701 catgcggtgc gcaatgttcc gttcggcgcg aacaatattt tccggcagac cctgcaggtc
     2761 gagaatgatc tcggcttccg gcagatcgaa gtccaaattg ccgcgagcgc ggcggcgctc
     2821 atagagcaag agagccagct cttccatgcg cttgaattca tccaccaggg cgctgtagcg
     2881 cgcgagacat tcggcatcgt ggtccaccag gatccggcgc acatccgtgt aggtcatccg
     2941 ttcatgactc cggatcactg agttgaaaaa acggctccga atgacgtcgc cctttgcgtt
     3001 aatctctata cagaccgttt tggtca
//
LOCUS       DASULZ010000795         2030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_600519, whole genome shotgun sequence.
ACCESSION   DASULZ010000795 DASULZ010000000
VERSION     DASULZ010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600519"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VFY96_16305"
     CDS             <1..512
                     /locus_tag="VFY96_16305"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608090.1"
                     /translation="EACLDGAFDPTFSDELLFDHATFLPLHFLNPGMAVPVVPVIFNA
                     LTPPMPAPSRCFAFGQCIGKFLHKTPLRIAVIATGGLSHWPGEAKHGKINITFDQQFL
                     ENLVNGDSAKLTAYSHEEIYREAGSGGHEIRTWIALAGAVQGWKAERVAYEPVVPWAT
                     GCGLVAFQQ"
     gene            557..1555
                     /locus_tag="VFY96_16310"
     CDS             557..1555
                     /locus_tag="VFY96_16310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5608091.1"
                     /translation="MKFSLHSLHGGVKNIAQIHQRARRAEELGYDGIFLGESHLSSID
                     SFQTLASCATITQRVLLGIAVTNMVFRHPTVLAGAAASLNEISKGRAILGIGTGDGPV
                     YTQGLKATPLKEFEQGVRLIRHLVQGNAATFPTGKVAIGFTINKPAPVYVSAEGPKGL
                     QLAGRSADGVILGTGFDLHVYTWAQEQIRKGAQETGRRERDVRIIAAGMLCVRDNGEE
                     ARRIVRNRIANRAHHNFRFTFETVPAGELAGVKRFMDAFDVMKPMEERVDPELVNDYL
                     VRRFSIAGNAQECIERIEELKLAGVSHLMLTPARKVYGETVEAFATKVMPHFKDRP"
     gene            complement(1575..>2030)
                     /locus_tag="VFY96_16315"
     CDS             complement(1575..>2030)
                     /locus_tag="VFY96_16315"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5608092.1"
                     /translation="LKTPDKKEERIARLKEIINPIFDYEEMARRCLGPYWRRRTPAEQ
                     EEFAKLFREFLENIYADRVDLYEGEKIVFNRETTDQDYAQVDSTVTNDKGEARSVVYR
                     LKRTDGQWKVYDAVIENVSVVNNYRSQFERVIAKSSYEELKRMLREKAQ"
BASE COUNT          425 a          584 c          556 g          465 t
ORIGIN      
        1 tcgaagcttg cctcgatggt gcattcgatc ccactttttc cgacgaattg ctattcgacc
       61 atgcgacgtt tttgccgctt cattttttaa atccgggaat ggctgttccg gtggtcccgg
      121 taatcttcaa tgcgcttaca ccgccgatgc ctgcgcccag ccgttgtttt gcctttggcc
      181 aatgcatcgg caagtttttg cacaaaactc cgctgcgcat cgccgtgatc gcgaccggcg
      241 gtctgtctca ctggccaggg gaagccaagc acggcaagat taacatcaca ttcgatcagc
      301 aatttctgga aaatttggtc aacggcgatt ctgcgaagct gactgcgtac tcacatgaag
      361 aaatttacag ggaggccggt tccggcggac atgaaatcag aacctggatc gcgctcgccg
      421 gcgcagttca aggttggaaa gcggaacggg tcgcgtatga gccagtcgtg ccctgggcaa
      481 ccggctgtgg cttggtcgca tttcagcaat agagtcgatc gggcgcagca tgctgcgccc
      541 ctactggtgt ctgctcatga aattcagcct gcacagtctt cacggcggcg tcaaaaacat
      601 tgctcaaatt caccagcgcg ctcgccgagc cgaagaactc ggctatgatg gaatttttct
      661 tggcgagagc catctgagct ccatcgattc atttcagacg cttgccagct gtgcgacgat
      721 cactcaacgc gtgctgctgg ggatcgcagt tacgaacatg gttttccgcc atccgacggt
      781 acttgcgggc gccgccgcct ctctgaacga aatctccaaa ggccgcgcca ttcttggcat
      841 cggcactggc gacggtccgg tgtacacgca aggcctcaaa gccacgccct tgaaagaatt
      901 cgaacagggg gtgcggttaa tccgccatct cgtgcagggg aatgccgcta catttccaac
      961 tggaaaggtg gctatcggat ttacgatcaa caagccggcg cccgtctacg tttcagcgga
     1021 agggccaaag ggtttgcagc tcgcaggacg gtcagccgac ggcgtgatcc tcggcacggg
     1081 attcgacctc cacgtttata cgtgggcgca ggaacagatc cggaaaggcg cgcaggagac
     1141 gggacggcga gaacgtgacg tccgcatcat cgctgccggt atgctctgtg tccgggacaa
     1201 tggcgaggaa gcgagacgca tcgtgcgcaa ccgcattgca aatcgcgcgc accacaattt
     1261 ccgcttcacc ttcgaaaccg ttcccgccgg ggaactcgcc ggggtcaagc gtttcatgga
     1321 cgccttcgat gtgatgaagc cgatggaaga gcgcgtcgac cccgagctcg tcaacgatta
     1381 cctcgtgcgc cgtttttcga tcgccggcaa tgcgcaggaa tgtatcgaga gaatcgaaga
     1441 gctgaaactc gccggcgtga gccacctcat gctgactccc gcgcggaaag tctatggcga
     1501 aaccgtcgaa gctttcgcca cgaaagtgat gccgcatttc aaagaccgac cgtgatctta
     1561 caccgagtcc tggcctattg agccttttct cggagcattc ttttcagctc ttcatatgag
     1621 gacttggcaa tcacgcgctc aaactgcgat cgataattgt tcacgacgct aacgttttcg
     1681 atcacggcgt catacacctt ccattggccg tccgtcctct tgagccggta aacaaccgag
     1741 cgggcttcgc ctttgtcatt ggtcacggtg gaatccactt gcgcgtaatc ctgatcggtc
     1801 gtttctcggt tgaaaacaat tttctccccc tcgtagaggt caactctgtc ggcgtaaatg
     1861 ttttccaaaa actcacgaaa cagtttcgcg aactcttcct gttccgccgg tgtgcggcgg
     1921 cgccaatacg gaccgaggca gcgccgcgcc atctcttcgt agtcgaagat cgggttgatg
     1981 atctccttca ggcgggcgat ccgttcttcc tttttgtcag gtgttttcag
//
LOCUS       DASULZ010000796         2613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1005590, whole genome shotgun sequence.
ACCESSION   DASULZ010000796 DASULZ010000000
VERSION     DASULZ010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005590"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="VFY96_16320"
     CDS             complement(<1..161)
                     /locus_tag="VFY96_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015861043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEX5608093.1"
                     /translation="MPIFRLVDDVVFPPPDYADPSGLIAVGGDLSSDRLLEAYRVGIF
                     PWYSEDQPIL"
     gene            267..638
                     /locus_tag="VFY96_16325"
     CDS             267..638
                     /locus_tag="VFY96_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5608094.1"
                     /translation="MMFKRIDHVALHVSDLDRSIKFYEQHFGFKHYFQHAAGGGLQIA
                     YLKLGDTVLELTHHPGETLSGFHFCLETDNFDQTVEQLQQDSVRLIRAPHDTAAREAR
                     ENGWRRVVFGGPDGEQIELRG"
     gene            703..1008
                     /locus_tag="VFY96_16330"
     CDS             703..1008
                     /locus_tag="VFY96_16330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608095.1"
                     /translation="MSNETNTIHVTCPCCDAALTIDPVLGVVLDHKLPVRPQMAVALK
                     DAVKAVNEEASRRDQKYQQIAEAEKNKSKVLEAKFQELFKKAKEEPVTKPLKDIDLD"
     gene            complement(1021..1452)
                     /locus_tag="VFY96_16335"
     CDS             complement(1021..1452)
                     /locus_tag="VFY96_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608096.1"
                     /translation="MGYIQANLLPDEEILYKARLHWIIFWKCCALVFLGAIFVDSANR
                     RRGGHPDRVVWRAVADFGLLHVGVRGDQQTGDYQNRISALKDLGVTPAACGNDFSRSK
                     RCWQDSRFRSVTLTGSGGVKEMFDNISAPLEFRRRIQGEAT"
     gene            1574..2572
                     /locus_tag="VFY96_16340"
     CDS             1574..2572
                     /locus_tag="VFY96_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX5608097.1"
                     /translation="MRAMVFEKPGKDLSLSEMDPPTPRAEQVLLRVHACAVCRTDLHV
                     VDGELLEPKTPLIPGHEIIGSVATIGAAVTRFKPGDRVGVPWLGWTCGECSFCRENRE
                     NLCDRAKFTGYTLDGGYAEYTVADQRFCFPIPGDYSDSEAAPLLCAGLIGYRSLVKAG
                     NGKRLGIYGFGAAAHIVAQLAKYQKREIYAFTRPGDSEAQQFALTLGAIWAGSSNELP
                     PVKLDAAIIFAPVGALIPQALKAVRKGGIVVSGGIHMSDVPSFPYALLWEERSICSVA
                     NLTRRDGAEFMELAPKVPVRTQVETFALEQANEALQRLRSGRIHGAAVLVMESSAV"
BASE COUNT          623 a          699 c          728 g          563 t
ORIGIN      
        1 aggatcggct ggtcctccga ataccacgga aaaataccga cccggtaggc ctcaaggaga
       61 cgatcgctcg acagatcacc gcccaccgca atgagtccgc tcggatcggc ataatccggc
      121 ggcgggaaaa ccacgtcgtc gaccaggcga aaaatcggca taggcaacag tccagcgcta
      181 ttttatctct ccggcgcaca attttcatgc cttgagtaag cttgcaaaaa cgactctgaa
      241 agacttatcc ttaatcgtgg agaaaaatga tgtttaagcg aattgatcat gtggcgcttc
      301 atgtctcgga tctcgaccga tcaattaaat tctatgagca gcacttcggg ttcaagcact
      361 attttcaaca tgccgccggt ggcggactgc aaattgccta tctcaaattg ggcgatacgg
      421 tgctagaatt gacgcatcat cccggcgaga cgctctcagg ctttcatttc tgccttgaga
      481 cggacaattt tgaccagact gtcgagcaac tgcagcagga tagtgttagg ctcatccggg
      541 cgccacacga tacagcagct cgggaggcgc gggaaaatgg ctggcgacga gtggtcttcg
      601 gcggacccga cggagagcag attgaattgc ggggatgagt ttcgcgtgaa ccgtaaggcg
      661 aaaggcgtga ggggatattc tgcgaccctt taggagtgag ccatgtctaa tgaaaccaat
      721 acgatccacg tgacgtgtcc atgctgcgat gcggcgctga cgattgatcc cgtgctcggg
      781 gtcgtactcg accataagct gccggtcagg ccgcaaatgg cggtggcgct caaggacgca
      841 gtaaaggccg tgaatgaaga agcgagccgg cgcgatcaga aatatcagca gatcgctgaa
      901 gcggaaaaaa acaaatcaaa agttctcgaa gctaaatttc aggagctttt caaaaaagcc
      961 aaagaagagc ctgtcaccaa gccgctgaaa gacatcgacc tggactagcc gagcgaattg
     1021 ctacgttgcc tcgccctgga tgcgccggcg gaattcgagc ggcgccgata tattatcaaa
     1081 catttccttc acgccgccgc tgccggtaag cgtgaccgac cgaaatcgag aatcctgcca
     1141 gcaacgcttt gatcgactga aatcgtttcc acatgccgca ggagtaactc caaggtcttt
     1201 cagcgcagaa atccggtttt gataatcacc cgtttgttgg tcaccgcgaa ctccgacgtg
     1261 gagtaagcca aaatcggcca cagcgcgcca aacaacccga tcaggatgac caccacgccg
     1321 acgattggct gaatctacaa aaatggcccc gagaaatacc agcgcgcagc atttccagaa
     1381 aatgatccaa tgcagtctcg ctttatagag aatctcttcg tccggcagta aattcgcttg
     1441 gatgtatccc acgcgcggcg ctccttgtta aactaattgc acgattctat cacgagtgac
     1501 tttccccgaa atcatcggca agtttaatcg caaagattgg tttcgggaag cagtttcgat
     1561 aaagagctaa ttcatgcgtg ccatggtctt tgaaaaaccc ggcaaagatt tgtccttgtc
     1621 ggagatggat ccgcctacgc cgcgcgccga acaggttttg ctccgtgtgc atgcatgcgc
     1681 ggtgtgccga accgatttac atgtcgtcga cggcgaattg ctcgagccga agactccatt
     1741 gattcccggg cacgaaatta tcggcagcgt cgctactata ggcgctgcag tgacgcggtt
     1801 taaacctggc gatcgtgttg gcgtgccgtg gttaggttgg acctgcggcg aatgttcatt
     1861 ctgtcgcgaa aatcgggaaa atctctgcga ccgagccaag tttacgggct acacgttgga
     1921 tggcggctat gccgaataca ctgttgccga tcagcgcttc tgttttccga tcccgggaga
     1981 ttacagcgac agcgaagctg cgccgttgct gtgcgccggt ctgatcggct atcgcagctt
     2041 ggttaaagca ggcaatggca agcgacttgg catctacgga tttggcgccg ctgcacacat
     2101 cgtcgctcag ctggccaagt accagaagcg cgagatctac gcgttcaccc ggccgggcga
     2161 cagcgaagcc caacagtttg cgttaacgct tggcgctatc tgggccggca gttccaacga
     2221 attgccgccg gtgaagctcg acgctgcgat tatctttgcg ccggtcggcg cgctcatacc
     2281 gcaagcgctc aaggccgttc gaaaaggcgg cattgtggtt tcgggcggca tccatatgag
     2341 cgacgttccg tcttttcctt atgcgcttct ctgggaagaa cggtcgatct gttcagtcgc
     2401 caatttgacg cgccgggacg gcgcggagtt catggaactc gcgcctaaag ttccagtgcg
     2461 cacgcaagtg gaaacgtttg cgctcgagca agccaatgaa gcgctgcagc gcctgcgttc
     2521 gggaaggatt catggcgcgg cggtgctggt gatggaatcg agcgccgtct aggttttctt
     2581 tcgagcctca cgtaagacgc gtaatcccca agc
//
LOCUS       DASULZ010000797         9236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1513299, whole genome shotgun sequence.
ACCESSION   DASULZ010000797 DASULZ010000000
VERSION     DASULZ010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 9236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9236
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513299"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..1972
                     /gene="polX"
                     /locus_tag="VFY96_16345"
     CDS             260..1972
                     /gene="polX"
                     /locus_tag="VFY96_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEX5608098.1"
                     /translation="MKDRFEIAAELRVIARLLTIKGENPFKVQAYERGARALETFQGD
                     FEKLVKAGRLKEIAGVGNALSAIIEEIYRTGECWLLEQLRQELPAGAVELSEVPGLSL
                     KKIIAVNEALHIETVADLKSACEQGLVSKVKGFGLKSQHKLLADIDKLETRKQDRSLL
                     LSHAREQAEAVLGYLRLCPALLEAEPAGALRRQNETVDEILIVAATDQPKLVIDHFLR
                     LPTLARTIELTDDRCVASLAAGIKVDLEAVPPDEFVATLHARTGAPNHIAKLAKIAHS
                     KGIDLTSTQARSKGGVRFKDENEIYQRLGLQYIAPELREDNGEIEAAMSGKLPEPVAL
                     QDICGMTHCHTVYSDGRNTIEEMALAAEAMGMRYLTITDHSPSAYYARGVSIDRLRAQ
                     WDEIARVQERVKIKLLRGTESDILEDGSLDYPDHYLAQFDIVIASIHSRHKMDADQMT
                     RRLLRALKLPVFKIWGHPLGRLIQSRPPFACRMDEVLDAVAGSRCAIEVNGDPRRLDL
                     APEWIRAARERGIKFIVSTDAHSTGALNYLPYGVSTARRGWLTRDEVLNTLDSESFMK
                     AVRP"
     gene            1969..2637
                     /locus_tag="VFY96_16350"
     CDS             1969..2637
                     /locus_tag="VFY96_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608099.1"
                     /translation="MSRVFILSPANCNGLRAQWVLKKSSRSDLAMRLRSAGVSLGEIF
                     TYLSALYFRGKLAYARNFAEPPSSCPGILIITPTAGLLAHDTLIRLPRLRGFGRVPIH
                     VKNQTYRAALRRSARELVTQIEPECEIVLLGSVATGKYLDILAPIFGSRLRVPAEFVG
                     MGDMARGGLLLRCVRENRQLNYVEVASVLRSVSKRMRGNQEIVTKLSGISHDDGIIGE
                     IGTR"
     gene            complement(2641..2994)
                     /locus_tag="VFY96_16355"
     CDS             complement(2641..2994)
                     /locus_tag="VFY96_16355"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5608100.1"
                     /translation="MEFTNLNEKIEEAGKENLRNVQLFGTDNFRSWMLHFPPGDSTPM
                     HYHQSPETFLVIAGKCSVKDIKGNERILEKNDIVFFPAKEYYQLTSIGSDTLVLFGNR
                     SEPFGVRPVRAEAKQ"
     gene            complement(3189..3389)
                     /locus_tag="VFY96_16360"
     CDS             complement(3189..3389)
                     /locus_tag="VFY96_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608101.1"
                     /translation="MIDTSRKGRRCRAKKMVRTHEAKIEPERRGTIVHELENLGRRMV
                     LVEWDDNSSLYVFPDEIEIEEN"
     gene            4177..5241
                     /locus_tag="VFY96_16365"
     CDS             4177..5241
                     /locus_tag="VFY96_16365"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol ring-cleavage dioxygenase"
                     /protein_id="HEX5608102.1"
                     /translation="MGQILGLGITHYPGLGTQGNLSRRIKICLADAALPAHLRSIDNW
                     PRPMRDQWGMDDGMTHSTAHRAEMIEGFRMTRRALDEFQPDFCVVWGDDQFENYRDDC
                     VPAFSVLAYDSAEFQPWLHNQRGINCWNEAREKSYTVAGHRSGGKHLASFLLNQGFDI
                     AYSYKPLHIGLGHAFANSVLYLDWDRMGFSYPLVPFTVNAYGRHLTGSQGVPPTPSMG
                     QKLIQVEEDPPGPQPWRCFQIGAATARAFANTPWKVALIASSSWSHSFLTEKHGRMYP
                     DVESDKRYFEALRNGEWETWRNTNLAQAEDRGHHELLNWFCLAGAMAELKRKPDEAVF
                     LESWITNSDKVFAVFRPPLP"
     gene            5269..5580
                     /locus_tag="VFY96_16370"
     CDS             5269..5580
                     /locus_tag="VFY96_16370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX5608103.1"
                     /translation="MIALQVMVQIKPEHLSEYLEAIRHDAEHSEKDESGCLRFDVLQD
                     RDDPHRFYYYEVYRDEAALEAHRQTLHFKLYAEKVQRWLAAPPERRLGKNIIPSDASW
                     R"
     gene            5781..6785
                     /locus_tag="VFY96_16375"
     CDS             5781..6785
                     /locus_tag="VFY96_16375"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5608104.1"
                     /translation="MILRPACCSSLTALLFLLLCSFPNEILGQEKIRLAHSALESSNS
                     VWYLAKERGLYKKYGLDADLLFIPSTTTSVSSLVAGDVQVGNASGGGVASAVVAGADL
                     VLVSCYLNSLPYELVVNENIKSAEDLKGKSIGISRVGSASDVAARALIRGVGLEPDKQ
                     VPIMQVGGPAERAASFRTGRIAGFPSPPGIIYLTKGMPFRILISTADFQQRFEFPYIC
                     ATTTRSYLARQRDTVKKVLMAHIETVQYFKTHKDESKRIIAKYSRISEEKYLEAAYMA
                     SAKLYDVVPLVTRQGTEVQIKEATSRKPGAQLRFEDLVDESIVRELEKSGFIDKIYKQ
                     "
     gene            complement(6994..8442)
                     /locus_tag="VFY96_16380"
     CDS             complement(6994..8442)
                     /locus_tag="VFY96_16380"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HEX5608105.1"
                     /translation="MLEFLRRPNLVLLMALLLIVGALFWPSDLTVAQPRSPATTPKYV
                     FLFLADGGGMAHLEIARQYRRQILNEDFVIVDKIMKEGSVGVMTTHAADSLSTDSAAA
                     ATALAGGCKANIGALGVCADGTVSVSAMELARRRGMRLALVTNATLYDASPAAFVCHV
                     PNRRDFAVIVSRYLDLAPDVLMGGGKEQFLAKRQNGSFHGDEADALGAFRKKNYHLVE
                     NKTELERASSGKLLGLFAAKDMSFEIDRDKTREPSVYDMTRAAIKVLHDHNPHGFFAF
                     IESENIDTASHLSDITSVIHDYREFDRAVGLAYEFYRKYPRETLILVTSDHETGGLGF
                     TLALKDLSSTAGSNQVAGTVADFKKLQSIDISLQKAAQILGPQPNAEAIEKMMREHFR
                     GFTLAPEFKEAIIRRQPFSRTLFGNTTANALGLMIANNTQIYWQNSAHTNQPVLVAAL
                     GASAEQFRGYYDNADFGRKLKAVIEGKKEQPL"
     gene            complement(8435..9154)
                     /locus_tag="VFY96_16385"
     CDS             complement(8435..9154)
                     /locus_tag="VFY96_16385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608106.1"
                     /translation="MTRSFIFAWLVHLYTGLGVFVGFGSLLAIDQGSFRETFFLMGIA
                     VLIDATDGTFARLARVKEVLPWFDGNLLDELVDYFNYVIVPCFFVFRANLLPAPDALW
                     LAMLPLLASAYGFCQMNAKTADNFFLGFPSYWNIVVFYLYVLQTPKWINAFVILGLCI
                     LVFVPIKYIYPSRSPRFRFQTNFLGSLWGAAIFYIVYRLPEPSTTVAMTSLLFPAYYT
                     ALSLWLEFRRLTTGTPRDPHA"
BASE COUNT         2165 a         2365 c         2611 g         2095 t
ORIGIN      
        1 gtcctcgggt ctttgaatcg gtttcgaacc ggctcgatca ctgccaccga gctatctaga
       61 tcagctggtt ggcgctttgc aatgtctgtc ttcccgtgtg cagactatcc cgcgatcgca
      121 attaatatac agcgtgtcag ttgcgaacta acgtgctgtg ggaggatgaa tcgccgttga
      181 aagccgcagg tcgttgtatg cccttgagct ggtgattgag catttataga ctgaatctct
      241 atgcgttcgg agacagccgg tgaaagatcg ctttgagatc gcagccgagt tacgcgtgat
      301 cgcgcgcctg cttacgatca aaggcgaaaa cccatttaag gtgcaggcct atgaacgagg
      361 ggcgcgcgct ctggaaactt tccagggcga tttcgagaaa ctcgtcaaag ctggacgcct
      421 gaaggagatc gccggtgtgg gtaatgcact ctcggcgatc atcgaggaga tctaccggac
      481 gggggagtgc tggctgctcg aacaactccg tcaggagctg ccggcgggcg ctgtggaact
      541 cagtgaggtt cccggtctga gcctaaagaa aattattgcg gtcaacgagg cactgcatat
      601 cgaaacggtg gccgacctga aatcggcatg cgaacaggga ctggtcagca aagtcaaagg
      661 gtttgggctc aaatcgcagc acaagctgct cgcggacatc gacaagctcg aaacccgcaa
      721 acaagaccga tctctgttgc tcagccatgc tcgtgagcaa gccgaagctg tccttggcta
      781 cttacggctt tgtccggcgc tgctcgaagc cgagccggcg ggagccctcc gacgtcagaa
      841 cgaaaccgtg gacgaaattc tcattgtcgc cgccaccgat cagccgaagc tcgtcatcga
      901 tcactttttg cgcttgccaa cgctcgcgcg gaccatcgaa ctcaccgacg atcgctgcgt
      961 tgcgtccctt gcggcgggta ttaaagtaga tctcgaggcc gtgccgcccg atgaatttgt
     1021 tgcgacactt catgcgcgga ccggcgcgcc gaatcacatc gcgaagcttg cgaaaatcgc
     1081 gcactcgaaa ggaatcgatc tcacatcgac ccaagcacga tcaaagggcg gagtgcggtt
     1141 caaagacgaa aacgagatct atcagcggct cgggcttcag tatattgcac cggaactgcg
     1201 ggaagacaat ggcgaaatcg aggctgccat gtccggaaag ttgccggagc cagtagcgct
     1261 ccaagacatc tgcggcatga cgcattgtca cacggtctat tccgatgggc gaaacaccat
     1321 cgaggaaatg gcgttggcgg cagaagcgat gggcatgcga tatctcacga ttaccgatca
     1381 ctcgccgagc gcatactatg cccgcggtgt ttcgatcgac cgtttgcgcg cgcagtggga
     1441 cgagatcgcg cgagtgcagg agcgcgtaaa gataaagttg ctcagaggaa ccgagtcaga
     1501 cattctcgaa gacggctcac tcgattatcc tgaccattat ctggcgcaat tcgatattgt
     1561 gatcgcgagc atccattcgc gccataaaat ggatgccgat cagatgaccc ggcgtctgct
     1621 gcgcgcgctt aagctgccgg tgttcaaaat ctggggacat ccgcttggca gattaattca
     1681 atcacggccg ccctttgcct gccggatgga cgaagtgttg gacgcggtag ctggctcgcg
     1741 ttgcgcaatc gaggtcaatg gcgacccgcg tcgtctcgat ctggcgccgg aatggattcg
     1801 cgccgcgcgc gagcgcggca ttaaattcat cgtttcaacg gacgcgcatt ctacgggcgc
     1861 gttgaactac ttgccgtacg gagtttccac tgcgcgccgt ggctggctta cgcgggatga
     1921 agtgttgaat accttggata gcgaatcttt catgaaggcg gtccgcccat gagccgagtt
     1981 tttattctgt cgccggcgaa ttgtaacggt ctgcgggcac agtgggtgct gaaaaagagc
     2041 agccgctccg atctggcgat gcgactgcgc agcgccggcg tctccttagg cgagatcttt
     2101 acctatctaa gcgccctcta tttccgcggt aagcttgctt atgcgcgaaa ttttgccgag
     2161 ccaccttcca gttgtccggg aattttgatc attacgccga ccgccggatt gttagcccac
     2221 gatactttaa ttcggctgcc gaggctcagg gggttcggcc gcgtgccgat ccatgtgaag
     2281 aatcaaacgt accgcgccgc gctccggcgt agcgcgagag aattagtaac tcagatcgaa
     2341 ccggagtgtg agatcgtgct gctaggaagt gtggcgacgg ggaaatactt ggatatcctc
     2401 gctccgattt tcggcagccg gcttcgtgtg ccggcggagt ttgtcgggat gggcgatatg
     2461 gcgcgcggtg gattgctgct gcgttgcgtt cgggaaaatc gtcagctgaa ctatgtcgaa
     2521 gttgcgtcgg tgctccgttc ggtgtcgaaa cgaatgcgcg gaaatcaaga gatcgtcacc
     2581 aaactgtcgg gaatctctca tgatgacggc attattggag aaatcggaac aaggtgagca
     2641 tcactgtttt gcttctgctc tcaccggccg gacgccaaat ggttccgagc gattgccgaa
     2701 cagaaccaga gtgtcggatc cgatactcgt cagctggtaa tattctttgg caggaaaaaa
     2761 aacgatatcg tttttttcca gtatccgttc attgcctttg atgtccttga cggagcattt
     2821 cccagcaatt acgaggaaag tctctggcga ttggtggtaa tgcatcggcg tgctgtcgcc
     2881 cggcggaaag tgaagcatcc acgagcgaaa attgtcggtg ccgaagagct ggacatttct
     2941 gagattttcc tttcccgctt cttcgatctt ctcgttgagg ttggtgaact ccatggcttc
     3001 ctccgtctgg cacgtgttcg cgctagatta tgggagtcag acggaggtgt caatttgtgc
     3061 gttagatcgt cagtggcgag gtgagtgcga gaggggagtg gattcaaaca acgagaggca
     3121 tagaaaacga agattcgatg atttcattac caaagatggc gtgatgagag gcggaggaga
     3181 catgctcttt agttttcctc gatttcaatc tcgtctggaa acacataaag cgatgagttg
     3241 tcgtcccact cgacaagcac catgcgcctg ccgaggtttt ccaactcatg tacgatcgtg
     3301 ccccggcgtt caggctcaat cttcgcttcg tgagtacgaa ccattttttt ggctcgacag
     3361 cgccgcccct tccgactggt atcgatcatc gtcatctccc ggctctgaaa agcggccaac
     3421 ggaaattccg cgcggcggat gatgatcacc ggctgagatc tcaactgccg atcacatcat
     3481 ccggttgaac aattattaca cactaagtgt aatacgggtg agcgagactt caagcctgca
     3541 ggacagaggt tagctcagtg ctttgttcat cctgataagc tcaggtccga ttgcgcgtaa
     3601 ggcttttcac aagcagagtg cgcaaaacga aagctgttaa gattttgttc ctattcgttt
     3661 cgttttcgct ctgaaaagca tttttgcaga caatcaagcc gcgcgagagc tcgtttgcgg
     3721 cattggcagc atgcagcgac tgagaatgac acttcacgac actttgcgca cttaagatgc
     3781 atgcaaaaag gacatggagc ttcaggcaaa ggcgaaaatg atactgttcg catcgaaatt
     3841 ggactgtgcc tttcgtgaat atttgaattc tcggctggag ttttcaacgc tgtgttaacc
     3901 gagttgcatc gaacgaatca gtattggctg gtcgtttcta aatgatttat ttttacgaag
     3961 caacagtttt cggaagcttt gcaaaaaaat tgtattgaat tctgtttttg cacacgtaat
     4021 gagttttttc agaacacgac agcgcaaccg ttgactcgat atttcgcaga ttttccgatc
     4081 tgcacgtttt gagattcgct cttgcgtcaa ggcctatgct cgtttaagag cattctagtt
     4141 ggttggggcg gctgcacgct cacagaagga gaggcaatgg gtcaaatcct cgggcttggt
     4201 attactcatt atcccggttt gggcactcaa ggaaatttga gccgtcgcat caagatctgc
     4261 ctggccgatg ctgcgctgcc ggcgcatttg cgctccatcg acaactggcc ccgaccgatg
     4321 cgcgatcaat ggggtatgga tgacggaatg actcattcca ctgcgcatcg agcggagatg
     4381 atcgagggtt ttcggatgac gcggcgcgcg ctcgatgaat ttcaacccga tttttgcgtg
     4441 gtgtggggag atgatcaatt cgagaactat cgggatgatt gtgtgcccgc attttccgtg
     4501 ctcgcctatg actcggccga attccaaccc tggctgcaca atcagcgagg tatcaattgc
     4561 tggaatgaag cgagagaaaa gagctacacc gtagccggcc atcgcagcgg cggaaagcac
     4621 ctcgcctcct ttcttctcaa tcaaggattc gatatcgcat acagctacaa gccgctgcac
     4681 atcggcttag gacatgcgtt tgccaattcc gttctttacc tggattggga tcgcatgggt
     4741 ttttcgtatc cgctcgttcc gttcacggtg aacgcctatg gccgccatct taccggcagc
     4801 caaggcgtgc cgccgacacc gtccatggga caaaagctga ttcaggtcga agaagatccg
     4861 ccaggaccgc agccctggcg ctgctttcag atcggtgccg cgacggcccg cgccttcgcc
     4921 aacactccct ggaaagtcgc attgatcgcg tcgtcgagct ggtcgcattc gtttctaacc
     4981 gaaaagcacg gccggatgta ccccgatgtc gagtccgaca agcgttactt cgaagccctc
     5041 cgcaacgggg aatgggagac ttggcgtaat accaatttgg ctcaggccga agatcgcgga
     5101 catcacgaac tgctcaattg gttttgcctc gccggcgcga tggcggagct caagcgaaag
     5161 cctgacgagg cggtatttct cgaatcatgg atcacaaatt ccgacaaggt gtttgcggta
     5221 tttcgtccgc cgctcccttg acgatgaact cggccgtgga ggtgcagcat gatcgcttta
     5281 caggtaatgg ttcagataaa accagaacat ctgtcggagt acctcgaagc tatacgtcac
     5341 gacgctgagc attcggaaaa agacgaatcc ggctgcctgc gttttgacgt gcttcaagac
     5401 agagacgatc cacaccggtt ttactactac gaggtttata gagacgaggc tgcactggag
     5461 gcgcaccgcc agacgctgca tttcaagctc tatgcagaga aagtgcagag atggttggcc
     5521 gcgccgcccg agcgtcggtt ggggaaaaat attattccat cagacgccag ctggcgttaa
     5581 ccaccgttca ctcgcggtcg gcggcagtgc cattatcagc gcaccttcat agcagctagg
     5641 ctggttcaat gacgttgcgg tttcgtcatg ccggcgaacg ccggcatcca gtttgtgttt
     5701 atcagagtat cataaaggcg catagatcca gcatgcccga gaatgactga agaggcgctt
     5761 gagttatcgc aggagggacg atgatcttga ggccagcttg ttgctcgtca ttgaccgcgc
     5821 tgttgtttct tttgctctgt tctttcccaa acgagatcct cgggcaggaa aaaatccgtc
     5881 tggcgcatag cgctttggag agcagcaatt cggtctggta tttggccaaa gagcgcgggc
     5941 tctacaaaaa atacggcctt gacgccgacc tgctttttat cccgagcacg acgacgagcg
     6001 tttccagctt ggtcgcgggt gatgtacagg tcggcaacgc ttccggcggc ggggtggcga
     6061 gcgctgtagt agccggagcc gatctcgtat tagtttcctg ctatctgaat tcattaccct
     6121 acgagttagt cgtcaatgaa aatattaaat cggccgagga tctcaaaggg aaaagcatcg
     6181 gcatcagccg cgtcggaagc gcatccgatg tcgctgcgcg cgcgcttatt cgcggagttg
     6241 gattagagcc cgacaagcag gtgccgatca tgcaagtcgg cgggccggcc gagcgggcgg
     6301 cgtcctttcg gacggggcgg atcgccggat ttccatcgcc ccctggaatt atttatctga
     6361 ccaagggcat gccgtttcgc attctcatca gcactgcgga ttttcagcaa cgattcgagt
     6421 tcccttatat ctgcgcaacc acgaccagaa gttatctggc gcgacaacgg gatacagtga
     6481 aaaaggttct gatggcgcac atcgagacgg tgcagtattt caaaacgcat aaggacgaaa
     6541 gcaagagaat tattgccaaa tacagtcgaa tctctgaaga aaagtattta gaagcggctt
     6601 atatggcgag cgcaaaactg tatgacgtcg tgccgctggt gacgcggcaa ggaacggagg
     6661 ttcaaatcaa ggaagcgacg agccgaaaac ccggcgcgca gcttcggttc gaagatctgg
     6721 tcgatgaaag cattgtgcgc gagcttgaga agagcggttt tatcgacaaa atttacaagc
     6781 agtgaagcgg tcttggggat ttggagtcat ggagtggtgg agtgagggtt aattgatccc
     6841 ggccgcatga aggtattgga gatccacaaa tcgctcagcc gccgggagcg gggggaatgg
     6901 cttgtgccgc aacttcagga gctcttcgag ccaagccgct gctaaaaaat tggtggaaga
     6961 cagctagcta gggaatgggt gtaacatgga agcctataac ggctgttctt tctttccttc
     7021 gatgaccgct ttcagtttcc tgccgaagtc tgcgttgtcg taataaccgc gaaattgttc
     7081 ggcgctcgcg ccgagggcgg caaccaggac cggctggttg gtatgtgcgg aattctgcca
     7141 gtagatctgc gtattgttcg caatcatcag gccgagcgca tttgccgtgg tatttccgaa
     7201 gagcgtccgt gagaacggtt gccgtcgaat aatcgcttcc ttgaattcgg gggctagcgt
     7261 gaaaccgcga aaatgctcgc gcatcatttt ttcgatcgcc tcggcattcg gctgaggtcc
     7321 gagaatttgt gccgcctttt gcagcgaaat atcgatggac tgtaattttt taaaatctgc
     7381 aaccgttccc gcaacctgat tgctgcccgc tgtgctgctc aaatctttga gcgccaaggt
     7441 gaatcccagg ccgccggtct cgtggtccga agtgacaaga atcaaagtct cgcgcggata
     7501 cttgcgataa aactcatagg ctaatccaac tgcgcggtcg aactcacggt agtcatgaat
     7561 cacagaggta atgtcggaca gatgcgacgc cgtatcgatg ttttcggact cgataaacgc
     7621 aaaaaacccg tgcgggttgt gatcgtgcag caccttgatc gccgcgcgag tcatatcgta
     7681 aacggaaggt tcgcgggttt tgtcgcgatc gatttcaaag ctcatgtctt ttgccgcaaa
     7741 cagtccgagg agcttgccgg agctcgcccg ctcgagctcc gttttgtttt cgaccagatg
     7801 gtaatttttt ttccggaaag cgccgagcgc atcggcttca tcaccgtgga aactgccatt
     7861 ttggcgtttt gctaaaaatt gttctttgcc cccgcccatc aaaacatctg gcgcgagatc
     7921 caagtaacgg ctgacaatca ccgcaaaatc gcgccggttg ggcacgtgac aaacaaacgc
     7981 ggcggggctc gcgtcataca gcgtggcatt ggtaacgaga gccaggcgca tgccgcgccg
     8041 gcgcgccaat tccatcgcgc tcacggagac ggtgccgtcg gcgcaaacgc caagagcgcc
     8101 gatattggcc ttgcaaccgc ccgcaagagc cgttgccgct gcggcggaat ccgtcgataa
     8161 cgaatcggca gcatgggtcg tcatcacgcc aacgctgcct tccttcatga ttttatcgac
     8221 gatgacaaaa tcttcgttca gaatctgccg gcggtattgt cgggcgattt ccagatgcgc
     8281 catgccgccg ccgtccgcga gaaagagaaa aacgtacttc ggagttgttg ccggcgaacg
     8341 cggttgagcc acggtcaggt ccgatggcca gaaaagcgcg ccgacgatca gcagcaatgc
     8401 catgagcaga accaaatttg gccttcgtag aaattcaagc atgtggatcc ctcggcgtgc
     8461 cggtcgtcaa tcgacgaaac tcaagccaca gtgacaacgc ggtataataa gcggggaaca
     8521 gcagtgaagt cattgcgacc gtcgtggacg gctcgggcaa gcgatagaca atataaaaaa
     8581 tcgccgcgcc ccagagcgat cccaaaaaat tggtctggaa acgaaatcgt gggctgcgac
     8641 tcggatagat atatttgatc ggaacgaaaa ctaaaataca taagccgaga attacaaaag
     8701 cattgatcca ttttggtgtc tgcagcacgt aaagataaaa aaccacgatg ttccaatagg
     8761 aagggaagcc aaggaaaaaa ttatctgcgg tcttcgcgtt catctggcag aaaccgtagg
     8821 cgctcgccag caatggaagc atggcgagcc ataatgcatc cggagccggc agaaggtttg
     8881 cacgaaaaac aaaaaaacag ggcacgataa cgtaattgaa atagtcgacg agctcatcca
     8941 acaaattgcc gtcgaaccag ggaagaactt cttttacccg cgctaggcgg gcgaatgtgc
     9001 cgtccgtggc atcgattagg accgcgatgc ccatcaagaa aaaggtctct cggaaagacc
     9061 cttgatcaat ggcgagaagg ctaccgaaac cgacgaagac gcccagtccg gtatagagat
     9121 gaacgagcca ggcgaatatg aaactcctgg tcatccaccg gatctatgtg gccgaagcgg
     9181 cgaagctcgg tctggctttc aagcgagcga tccactcgac ggaacgcttg tacgtg
//
LOCUS       DASULZ010000798         3326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_600637, whole genome shotgun sequence.
ACCESSION   DASULZ010000798 DASULZ010000000
VERSION     DASULZ010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600637"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VFY96_16390"
     CDS             complement(<1..195)
                     /locus_tag="VFY96_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608107.1"
                     /translation="MENVVGIFKEKRNADSAMEEMRAAGFAPDKLILLTPGSSADTLR
                     RTPSEDAEQPGMGMAIGGVAG"
     gene            complement(352..915)
                     /locus_tag="VFY96_16395"
     CDS             complement(352..915)
                     /locus_tag="VFY96_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013905634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX5608108.1"
                     /translation="MLSVGHKVTDFECDAYHHEGIKKIRLSSYEGKWVVLLFYPGDFT
                     FVCPTELADAAAHYDQFTTVGAEILSVSTDSAFAHKAWRDVSPSLKEVRFPMVADSAG
                     KLSRYFGTYVEEFGICVRASFIIDPDGVVRACEFHDNRIGRNIDEILRKVQAAIYVRN
                     HEDELCPANWRPGAKTLKPTAELVGKI"
     gene            1134..1886
                     /locus_tag="VFY96_16400"
     CDS             1134..1886
                     /locus_tag="VFY96_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013555555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX5608109.1"
                     /translation="MTEIFRLGATADLHCKTSSAGALKDMFARLAENVDAVALCGDLV
                     DYGLPAEAEILLEELNPVLRKKIPTLAVFGNHEFESGKADQVQKILTIGGITVLDGSA
                     CEINGIGFAGIKGFCGGFGARVLEPWGESVIKRFVREAVDEALKLESALARLRTSQRI
                     ALMHYAPIVQTVSGEPPEVYPFLGCSRLEEPLDRYAVTAAIHGHAHRGSAEGHTKSGI
                     PVFNVAMGLMELASADHRPYRILELPVPRRLS"
     gene            complement(1813..>3326)
                     /locus_tag="VFY96_16405"
     CDS             complement(1813..>3326)
                     /locus_tag="VFY96_16405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5608110.1"
                     /translation="GLFNFSRDEILYQSVERLFPERSAFERVHGHTLSQLQESGFWRG
                     EGEYRRRDGSVFTAETTQTWLSTDQGDYIIVLVRDLSESKERDEIVHWLNEELARRFD
                     ERASELIARGAELQRAETERRQTERLLNVLMRNITNNAILFLSAEGYVIHSKGTEQLL
                     GYVAEDLDGILFSRLFKTAPEDQRRWSAMLEGAKQQSTVREYDWLLRKDGSQLHVRVE
                     IVALRDEAGALGGYIGFLRDDTEQKRVRDQLREKEHLASIGTATAMLAHEIRNPLNGM
                     STTVQFLERSLQNNFEPTKEMVISTLHDLKSEIARLQTLLAEFHVISQPQQMTSQPVQ
                     LAELLRTVMPLAVPEFFNDGFKIVEELDPKLPLVRGDHDKLKQVFLNIAKNAFEAMPD
                     GGTLTVKTYWRDQTICIEISDTGTGIPDGLNVFDLFRSTKAHGTGLGLAIARQIVLAH
                     GGAIEYTSKLGAGTTFQVTLPPMNSAATRARIASAAQATPESDRGGDPLRQAP"
BASE COUNT          712 a          928 c          902 g          784 t
ORIGIN      
        1 accggccaca ccgccgatcg ccatgcccat ccccggttgt tcagcatcct cgcttggcgt
       61 gcgccggagg gtgtcggccg agctgcctgg cgtcagcaga atcaatttgt ctggagcgaa
      121 acccgcagca cgcatttcct ccatcgctga atcggcattt ctcttttctt taaatattcc
      181 aacgacgttc tccatgttgt cctcccggtg ttgacttgtg caaggcacaa gccaagcacg
      241 tccgtaccga gccacttctg tgcgcctcgc caagtggttt gagtagaaaa gaactgcagc
      301 agtaccgagc gggaccgaag cagtccttgg cgttggaaat tgacttctga atcagatttt
      361 tcccaccagt tccgccgtcg gcttcagagt tttggcgccg gggcgccaat tggcgggaca
      421 gagttcatcc tcatggttgc ggacataaat cgctgcttgg acctttctca aaatttcgtc
      481 aatattgcgc ccgatccggt tgtcgtgaaa ctcgcaagct cggaccaccc catcggggtc
      541 tatgataaag ctcgctcgca cacagatacc aaattcttcg acgtacgttc cgaaatagcg
      601 agaaagtttt cccgcgctgt ccgcaaccat ggggaagcga acttctttca gcgagggtga
      661 gacatcgcgc caagccttgt gcgcaaaagc gctgtcagtg ctaacgctca atatttcggc
      721 gcctacagtc gtgaactgat cgtaatgcgc cgcggcatcc gccaactcgg taggacagac
      781 gaatgtaaag tctccggggt aaaacaacaa tacgacccat ttgccctcgt atgaagagag
      841 acggatcttt ttgattccct catggtgata ggcgtcacat tcaaaatcag tgaccttgtg
      901 tcctacggat aacatgtgag ctatgcgtta cgcttcggcc cgcgcgattc acgaaatagc
      961 agcaatggct ataccattac aaaagtatca tttcattgtc gtttacggtg cgaatccgcc
     1021 cccggatcct cccgaggcgt tccgttgtcg ggacagccag tgttctatgg ccgcgacaat
     1081 gactctcacg ctgtcgggcg gcgtttggca cagcagttgc cgcagtagaa ggagtgactg
     1141 aaatctttcg actcggtgcg acggcagatc ttcactgtaa aacaagctcg gcaggcgcat
     1201 tgaaagacat gtttgctcgt ctggcggaga acgtcgatgc tgtggcgctc tgcggcgacc
     1261 tcgttgatta cggtcttccc gccgaagcgg aaattctgct ggaggagctc aacccagttc
     1321 tgagaaaaaa aatcccgacg ctagcggtgt tcggcaatca tgaatttgag tcgggtaagg
     1381 ccgatcaggt ccaaaaaatc ctgactatcg gtggcatcac ggtgctcgat ggtagcgcct
     1441 gtgagatcaa cggcatcggg ttcgccggca tcaaggggtt ctgcggggga ttcggtgcgc
     1501 gcgtgctcga gccgtgggga gagtctgtca tcaaacggtt cgttcgtgaa gcggtggacg
     1561 aggcgttgaa attggaatct gctctagctc ggctcaggac atcgcagcgg attgcgttaa
     1621 tgcattatgc tccgatagta cagaccgttt ccggtgagcc gcccgaagtc tatcctttcc
     1681 ttggatgcag caggctcgaa gagcctctcg accgctacgc ggtaaccgcg gccattcacg
     1741 ggcatgcgca tcgcggcagt gcggagggtc acacgaaaag cggtattcca gtcttcaacg
     1801 tcgcgatggg gcttatggag cttgcctcag cggatcaccg cccctatcgg attctggagt
     1861 tgcctgtgcc gcggaggcta tcctagcgcg cgttgccgcc gagttcatcg gcggcagagt
     1921 cacctgaaac gttgttccgg cgccgagttt gctcgtatac tcaatcgctc cgccgtgagc
     1981 taaaacaatt tgacgagcaa tagcgagtcc aaggcctgtg ccatgagctt tggtcgatcg
     2041 aaacagatcg aaaacattta gcccgtccgg tatgccggtc cccgtgtcac tgatttcgat
     2101 gcagatcgtt tggtcacgcc aataagtctt taccgttaat gttccgccgt caggcattgc
     2161 ctcgaacgcg ttcttggcaa tatttaaaaa aacctgcttg agtttgtcgt gatcgccgcg
     2221 aaccagcggc aatttggggt cgagctcctc gacgatctta aatccgtcgt taaaaaattc
     2281 cggcacggcg agcggcatga cggtacgcaa cagctcagcc agctgcacgg gttgagaagt
     2341 catctgctgt ggttgagaga tcacatgaaa ttcggcaagc aatgtttgta gtcgggcaat
     2401 ctcgcttttc agatcgtgca atgtactgat gaccatctcc ttggtcggtt cgaagttgtt
     2461 ctggagcgaa cgttccaaga actgcacggt cgtggacatc ccgtttaacg gatttctgat
     2521 ctcatgagcc agcatggcgg tagcggtgcc gatgctcgcc agatgctcct tttctctgag
     2581 ctggtcacga actctttttt gctcggtgtc gtcgcgcaaa aacccgatat acccgccgag
     2641 cgcgcccgct tcgtcacgca acgcgacaat ttcgaccctg acgtggagtt gtgaaccgtc
     2701 cttgcgcagc agccaatcat actcccgcac cgtcgattgc tgcttcgcgc cttcgagcat
     2761 cgcgctccat ctgcgctgat cctccggcgc agttttgaaa agccgcgaaa aaagaatgcc
     2821 gtcgaggtcc tcggccacat aacccaggag ttgctcggtc cccttcgaat ggatgacgta
     2881 accttccgcg ctaagaaata gaattgcgtt gttggtgata tttcgcatca gcacgttgag
     2941 gagccgctcg gtttgccgac gctcggtttc ggctcgctgc agctcggcgc cgcgggcgat
     3001 caattcggaa gcgcgctcgt caaatcgacg cgccagctcc tcattcaacc aatggacgat
     3061 ttcatcacgt tccttgcttt cgctgagatc acgcaccagt acgatgatgt aatcgccctg
     3121 atccgtggaa agccaagttt gcgtcgtttc tgccgtgaaa accgagccgt cgcgacgtcg
     3181 atattcaccc tcaccgcgcc aaaagcctga ctcctgtagc tggcttaacg tgtgcccgtg
     3241 cacgcgctcg aatgcagacc gttcggggaa tagccgctcg acactttggt agagaatttc
     3301 gtctcgtgaa aaattgaaca agcctt
//
LOCUS       DASULZ010000799         2496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_295382, whole genome shotgun sequence.
ACCESSION   DASULZ010000799 DASULZ010000000
VERSION     DASULZ010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295382"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="VFY96_16410"
     CDS             <1..1051
                     /locus_tag="VFY96_16410"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX5608111.1"
                     /translation="LVGFLSRSDRAHVGLFGTTRRKRTSTTSDSFIQENSIAWYAQQE
                     VRPAAWLGTQLGLRLDKFYFDVRNDRTPADPSTGSIRGTDQGFIASPKLNVILSSFVE
                     NTWQKRSEIYLNFGGGYHSNDARDVVSSTADKPVLPRALGSELGFRTKLFDRFEIGID
                     YWRRHLYSELVWAGDDGPTEPGRTQCHGLEGEFRYQILEWLSAELDISYSWAKFVKSG
                     AAVPLAPRFLAFGVFTARHASGLERRIQMRSVGDRWGDQARTATIHGYTIFDLLAKYK
                     WNRFEFLFSISNIANKKWRAAQFFHSSQLQGNPRFPSEAAPVNDIHFTPGEPLTIKAG
                     MTIHYGSDGNEISCD"
     gene            1103..2344
                     /locus_tag="VFY96_16415"
     CDS             1103..2344
                     /locus_tag="VFY96_16415"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5608112.1"
                     /translation="MPATAPPLNQSISTATNSPQRNAVFGIVCGAHFVNHFQSAMLGV
                     IYPLMMTELGMSYIAIASLAAVYNTLGSIFQAAYGFVVPYVRRGVILGLGNCLLGLSV
                     AGAGFASNFNHFFTARLIGGLGSSPQHPVGSSMLASHFGAARGRALAFHSTAGQIGSL
                     LAPLLAATMVSFLGWRGVFWAVGGFTTFMGAICFVFRDSLRSAPLETKKSRLTPMGWE
                     AYKACFKDKNILLVSLVFMAGAAGRGQDINEVYIIPHFVRDFNLSITYGAFLFTFIQV
                     GSLIGPFIWGWISDRFNRKLVIQASLLMSALCTLWLAWQKDISAGLFFNLVIYGAAVT
                     SRQTLTQALLADLVGDELFDAAFSLYYFIGFVSIPFWTVITGGVMTHYGFGPAFTVIS
                     TSYLLAMSLLGLLRTPVKRRA"
BASE COUNT          499 a          713 c          663 g          621 t
ORIGIN      
        1 cttggtcggt ttcttgtcac gaagcgaccg cgctcacgtc ggcctctttg gcacgacgcg
       61 gcggaaacgc acgagcacga ccagcgactc attcatccaa gagaacagta ttgcctggta
      121 tgctcaacaa gaagtaagac ccgcggcctg gttaggaaca cagctcggcc tgcggctcga
      181 caagttttat ttcgacgtaa gaaacgatcg aacaccggcg gacccgtcaa ccggttcgat
      241 tcgtggcact gaccagggtt ttatcgcaag tcccaaactg aacgtgatcc tctcttcctt
      301 cgtggaaaat acctggcaga aacgctccga aatttatctt aattttggcg gcggttacca
      361 tagcaacgac gcccgcgatg tagtttccag cacggctgac aagccggtct tgccccgcgc
      421 cctaggcagt gagctgggct ttagaaccaa gcttttcgac cgcttcgaga tcggcatcga
      481 ctattggcgt cggcacctct acagcgagct tgtctgggcg ggcgacgacg gcccgacgga
      541 gccaggtaga actcaatgcc atgggcttga aggcgaattc cgttatcaga ttttggagtg
      601 gctcagcgcc gagctggata tctcctacag ttgggcaaag ttcgtcaaga gcggcgcggc
      661 ggttccgctt gccccgcgtt ttctcgcctt tggcgttttc accgcgcggc atgcttcagg
      721 gctggaacga cgaattcaaa tgcgttccgt cggcgatcgg tggggcgacc aagcgcgcac
      781 tgccacgatc cacggctata cgatcttcga cctacttgcc aaatacaaat ggaaccggtt
      841 tgagttcctt ttctcgatta gcaatatcgc caacaaaaaa tggcgcgcgg cgcaattttt
      901 tcatagctcc cagctgcaag gtaatccaag gtttcccagc gaagcggctc cggtgaacga
      961 tatccatttc acgcctggcg agccgctcac gataaaagcc ggcatgacca ttcactatgg
     1021 tagcgatggg aacgaaattt cgtgcgattg attgcgccgg gacgactgtg cggccgaacg
     1081 aactgacgga gtaaattttt taatgcctgc gaccgcacca cccctgaatc aatcaatttc
     1141 tactgcgacc aactcgccgc aacgaaatgc cgtgttcggc atcgtctgtg gcgctcactt
     1201 cgtcaaccat tttcaatcgg ccatgctcgg agtgatctac ccgttgatga tgacagaact
     1261 cggtatgagc tacatcgcca tcgcttcgct ggcagcggtg tacaacaccc tgggcagcat
     1321 ctttcaagca gcgtacggct tcgtcgtccc gtatgtgcgc cgtggcgtca ttcttgggct
     1381 aggaaactgt ctgctgggcc tgtccgtcgc tggcgcaggg ttcgcctcga atttcaatca
     1441 tttctttacc gcacggttga tcggcggtct tggctcaagc ccgcaacatc cggtcggatc
     1501 gagtatgtta gcgagccatt tcggcgcggc gcgcggccga gccttggcat ttcactcgac
     1561 cgcgggacag atcggaagtc tgctggcgcc actgctcgct gccacaatgg ttagctttct
     1621 cggctggcgc ggcgtcttct gggcggtcgg cgggtttacc actttcatgg gcgccatctg
     1681 ctttgttttt cgcgacagct tgcgttcggc ccctctcgaa acaaaaaagt cgcgtctgac
     1741 gccgatggga tgggaggcct acaaagcctg tttcaaagac aaaaacattc tgctcgtgtc
     1801 gctggttttt atggccggag ccgcaggtcg cggccaggat atcaatgaag tctacatcat
     1861 tccgcatttt gtccgcgatt ttaatttgag cattacctac ggcgcgtttt tatttacctt
     1921 cattcaagtg ggcagcttga tcggtccatt tatctggggt tggatttccg atcgtttcaa
     1981 ccgcaagcta gtgatccaag cctcgctttt aatgtccgcc ttgtgcaccc tctggctcgc
     2041 ttggcaaaaa gacatctccg ccggattgtt ttttaacctg gtcatctatg gcgcagcagt
     2101 aacgtcgcga caaaccttga cccaggcgct gctcgccgat ctcgtcggcg acgagctttt
     2161 cgacgccgcg tttagtctct actacttcat cggattcgtc tcgattccgt tttggaccgt
     2221 gatcactggc ggagtcatga cccactacgg ttttggtccg gcattcacgg taatatcaac
     2281 atcctatttg ctcgcgatga gcctgctcgg cttgctgcga actccagtaa aacgtcgcgc
     2341 ttgagttgaa gtatgcccaa gcaactttca ttcgcttctt ccttcgtagc gtcagcgctg
     2401 gtgcatctta tcggcttggg actcgcagtc gcgctgctca ctcagcggag ttatcgctac
     2461 caaccgaatt tgattcccgt gtcgctgtgg gagctc
//
LOCUS       DASULZ010000800         3679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_803031, whole genome shotgun sequence.
ACCESSION   DASULZ010000800 DASULZ010000000
VERSION     DASULZ010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803031"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VFY96_16420"
     CDS             complement(<1..267)
                     /locus_tag="VFY96_16420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608113.1"
                     /translation="MDVGTTHEDLERFLAEHHLVGAGLRREGRTTPRQGNTSAHWRWD
                     GIYRGLMRSGEIVTVGPNGMTGMRSVVGIEARKFPIWMNAQILMP"
     gene            complement(386..1540)
                     /locus_tag="VFY96_16425"
     CDS             complement(386..1540)
                     /locus_tag="VFY96_16425"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5608114.1"
                     /translation="MPRQYKCISADGHIDLNPDIWRDRVQIKHRERAPKRIKMANGSD
                     AVVIDGGKPNTIGLTRSVRVAHADLAKQVPTFETCAGTGAPEQRLAEQDQDGIDCEIL
                     FSQITFVLRQTQDDDLYRDCIRAYNEFLAEEYMAPAPDRLVCMGTLPTTGVDDAIKEL
                     EHCVDLGMKGVKLDRYPSGKSYPTPEDDKFWAAALDLGIALTNHNTGNMGSGKGEPDY
                     LYPKDPGHDVHQKDAFKFFTRFTNDCMTASLQMAFAGVWDRFPDLKFYWAETMIGWLE
                     YGLWQVDDHYTRYIGMIHEAWGLPKLERMPSEYLKDRNYWGFLRDPVGVKRRQCIGYD
                     KIMWGTDFAHAASDFPNSRKHLAEDFAGVPENELRAMLVDNCVKYFHLDR"
     gene            complement(1608..2666)
                     /locus_tag="VFY96_16430"
     CDS             complement(1608..2666)
                     /locus_tag="VFY96_16430"
                     /inference="COORDINATES: protein motif:HMM:NF014136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5608115.1"
                     /translation="MSDPHSLPDTPELKALHARLAEYSLAGHWQAREKNPELVPYLWP
                     WAMIYSCLMESGEVVQLGGIDDAAKRRTVQLVNPALGTKKATTRTLQMSIQLVKPRER
                     AECHRHSAAALRFVVEGDGTAYTNVNSEEMLMEPGDLVLTPNWSWHDHFNAGKNNVVW
                     LDVLDSHLTRYLDAGFEENYGEGPAQPIVRRDGACKQRYGAIRPRVMSSSSAALPYNY
                     KWRDTLQALNETAAGGPLDAHDGVLLEYSHPLTGGSTMPTIGCWVQMIPPGESTAPHR
                     HTSSTIYHVVEGEGITSVGKSKSAMKDLTWRTRDCFFVPSWNWHSFKNASSKTAAIIF
                     SVTDRPVLESLGLFREEE"
     gene            complement(3099..>3679)
                     /locus_tag="VFY96_16435"
     CDS             complement(3099..>3679)
                     /locus_tag="VFY96_16435"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5608116.1"
                     /translation="TGLGGTSDVALRIALEAVGENPKDFIIVALGAPQLMSGLESGSI
                     EAAQLNSPLNYHAKKKGFRELLDISAHVQMPLGGLTASNASIQNRTNELRRVIRSLQF
                     AKRTLLQSKEKAIDLIMRTIRVDREVADEMFAENRRSAAGNGVPTREGIEQIVKSLQL
                     LGQFTGRKISFEEITDARIARDVAKELGYKVD"
BASE COUNT          792 a         1068 c          955 g          864 t
ORIGIN      
        1 cggcatcaaa atctgcgcgt tcatccagat gggaaatttt ctcgcttcga tgccgacgac
       61 cgagcgcatg ccggtcatgc cattgggacc gacggtaacg atctctcccg agcgcatgag
      121 gccgcgataa ataccgtccc atctccaatg cgccgaggtg ttgccctgcc gcggcgtggt
      181 gcgtccttcc cgtctgagcc ccgcgccaac gagatgatgc tcagccaaaa agcgctcgag
      241 atcctcgtga gtagtgccaa cgtccatcaa aagcttatcc tctacaactg ctatgcgtct
      301 ggcagtttga acgtatggag cagctggcac gttttgaacg gttcaagtcg ttctagcagc
      361 ttaagccgtt caaatcgtcc gccactcatc gatccaggtg aaaatatttt acgcagttat
      421 ccaccagcat tgcgcgcagt tcgttttccg gcacgccggc gaaatcttcg gccaaatgtt
      481 ttcgtgaatt agggaaatcg ctcgccgcgt gggcgaagtc cgtgccccac atgattttgt
      541 catagccgat gcattggcgc cgtttgaccc ccaccggatc gcggagaaag ccccagtaat
      601 tccgatcctt caggtattca ctcggcatac gctccagttt cggcagtccc cacgcttcgt
      661 ggatcattcc gatataacgc gtgtaatggt catctacttg ccagagcccg tattccaacc
      721 acccgatcat ggtttccgcc caatagaatt ttaggtcggg aaaacgatcc cacacgcccg
      781 cgaacgccat ctgcaatgaa gcagtcatgc aatcgttcgt aaaacgagtg aaaaatttaa
      841 acgcgtcttt ctgatgcacg tcgtgacccg ggtctttcgg ataaagataa tcgggctcgc
      901 cttttcctga tcccatattg cccgtattat gattcgtcag cgcgatgccc aagtcgagcg
      961 ccgcggccca gaatttgtcg tcctccggcg tcggatagct tttgccgctc ggataccggt
     1021 cgagcttcac gcccttcatt ccgaggtcga cgcagtgctc cagctcttta attgcgtcat
     1081 ccactcccgt ggtcggcagc gtgcccatac aaaccaatcg atccggtgcc ggcgccatat
     1141 actcttcggc gagaaactcg ttataggcgc ggatgcaatc acgataaaga tcatcgtctt
     1201 gggtctgacg cagcacaaac gtgatctgtg aaaagagaat ttcgcaatcg atgccgtcct
     1261 gatcctgttc ggccaaacgc tgctcaggcg cgccagtccc agcgcatgtt tcaaaggtcg
     1321 gaacttgctt ggcaaggtcc gcgtgcgcta cacgcacgct gcgcgtgagc ccaatggtat
     1381 tcggcttgcc gccgtcaatc acgaccgcgt cggagccgtt ggccatcttg atccgcttgg
     1441 gcgcgcgctc gcgatgctta atttgtaccc gatcgcgcca gatatcggga ttgagatcga
     1501 tatgcccgtc agcggaaatg catttgtatt gtcgtggcat gataaggcct ctctctattg
     1561 gagtgctggt ttcggaaact caatactcca aactccacca ctccatccta ttcttcttcc
     1621 cggaacagcc ccaggctttc gagcaccggc cgatcggtta cggaaaatat gattgcggcg
     1681 gttttgctcg aggcattttt gaaagagtgc cagttccagg acggaacgaa aaaacaatcc
     1741 cgtgtccgcc acgtgaggtc tttcattgcg gacttcgatt ttcctacgct tgtaattccc
     1801 tcaccctcaa cgacatgata gatcgtgctg ctcgtatgac gatgtggagc ggtgctctcg
     1861 ccgggaggaa tcatctgaac ccaacagccg atcgtcggca tggtcgagcc gccggtgagc
     1921 gggtgactgt actccagcaa cacgccgtcg tgagcatcta aagggccgcc agcagccgtt
     1981 tcattcagcg cctgcagggt gtcacgccat ttgtagttat agggcaacgc tgcgctactg
     2041 ctactcatca cgcgggggcg aatcgcgcca tagcgctgct tgcaggcgcc atcgcgcctg
     2101 acgatcggtt gcgccggacc ctcaccgtag ttctcctcaa agcctgcatc gagataacga
     2161 gtcaaatgcg agtcgagcac atcgagccaa acgacattgt tttttccggc attgaaatga
     2221 tcatgccagc tccaattggg cgtcagcaca agatcacccg gctccatcag catctcttcg
     2281 ctgttaacgt tggtgtaggc ggtgccgtcg ccttcgacca cgaagcgtaa cgccgccgcg
     2341 ctatggcgat ggcactccgc gcgctcgcgc ggcttgacga gctggatcga catctgcagc
     2401 gtgcgcgtag ttgctttttt cgtaccgagc gccggattga ccagctggac agtgcgccgt
     2461 tttgctgcat catcgatgcc gccgagctga accacctcgc ccgattccat caagcacgaa
     2521 taaatcatcg cccacggcca aaggtacggc acaagctcag gattcttttc gcgcgcctgc
     2581 caatgaccgg ccaaggaata ttctgcaaga cgggcgtgaa gggctttaag ctcgggcgtg
     2641 tcgggaagtg aatgagggtc gctcataatg tgcctctgat agatttcaga acctactggt
     2701 caattcaatt cgcataagct caatcaaggg aagattcctc gctcttcgct cggaaggaca
     2761 aatattatgt tgtcatctcg aacccattcg attcactcag ggtaaactcc gtgagagatc
     2821 tttcccgaat tgaaccattg cgtcatgcca acagtatttg agaaaaaaac aattttccag
     2881 tctggcgttg acccgagtag ttcgagtcgc cctttgatcc ggagctgccg gagacaaagc
     2941 tccgcagcca ggtgttttgc gtcgagccag cggggtatac ccttcgccca aagaaacgag
     3001 attcgtttca gtctgtagcg gcaaaagctc gcaatagcgc cggcaagacg gatttttttc
     3061 gccaacgcca gtgatttcaa tcattggcaa ggaaaacatc aatcgacttt atagcccaat
     3121 tccttcgcca cgtctctggc gatccgcgcg tcggtgattt cctcgaagga gattttcctg
     3181 ccggtgaatt ggccgaggag ctgcaacgat tttacaatct gttcgatgcc ttcacgggtt
     3241 ggcacaccgt tccccgccgc gctgcgacga ttctccgcga acatttcgtc ggcgacttcg
     3301 cgatccacgc gaatggtgcg cataatcaaa tcgatcgcct tctctttcga ttggagcaac
     3361 gttcgtttag caaattgcag tgatcggatg acgcgcctga gctcgttagt gcgattctgg
     3421 atcgaggcgt tcgacgcggt cagtccgccc agcggcatct ggacatgcgc gctgatgtcg
     3481 agcaactcgc gaaagccttt tttcttagcg tgataattta gcggcgagtt cagttgcgcc
     3541 gcttcgatgc tgccgctttc caagccgctc atgagctgcg gcgcccccaa agcaacaatg
     3601 ataaagtctt tgggattttc tccgacggct tcgagcgcga tgcggagcgc gacatccgac
     3661 gtgccaccga gaccagtaa
//
LOCUS       DASULZ010000801         4270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_396570, whole genome shotgun sequence.
ACCESSION   DASULZ010000801 DASULZ010000000
VERSION     DASULZ010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396570"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..1283
                     /locus_tag="VFY96_16440"
     CDS             189..1283
                     /locus_tag="VFY96_16440"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5608117.1"
                     /translation="MIIDGDGHFFETEEIFDKYMEPALRNYRPRLLKDDQGHNFWVVD
                     GQTPYKRPSIKGAGAPGTAAPPAEAIQSARRASPGSQTLTNLNERLKDLDREEIDLQF
                     IYPSFLLHVNAWLDGILAMGVCRAYNTWLAETCNRAPDRLRAVGVVSLQDPAGAAKEL
                     ERLRDLAVSAVMINGTAGAKRLDHPDHEVFFAEANRLQMPIAVHFSLQFPVVDKLFEH
                     HFPNRVLAGILPIMAGLTSVLCSGLLDRYKNLKFAFLEGGISWVPAHVERMDDHFENP
                     RYGARDLISHPPSDYLKDGRIFFGCEGNESFLSRILDEVGEDLFIFSSDYPHADRTEG
                     TAKILRDRTDIPLTVKSKLLEQNARQFYSI"
     gene            complement(1346..1705)
                     /locus_tag="VFY96_16445"
     CDS             complement(1346..1705)
                     /locus_tag="VFY96_16445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608118.1"
                     /translation="MVFFSIVSVFFSSIFSVDFSLAFFVRLLLAFAAFDFVLPVSSLV
                     ELDFSVAVSFFFSVAGGVAGLAGVAGVAGVAGVAWAKLAALNMNVTATKIKALIAFFT
                     ESPPFGWLDAGLLLVFK"
     gene            complement(2149..2805)
                     /locus_tag="VFY96_16450"
     CDS             complement(2149..2805)
                     /locus_tag="VFY96_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608119.1"
                     /translation="MAALKANEFINQLAATREAFWEQMNAERKSYGAEKPLTPAVVLR
                     RLQFGVVMERKAAEVTAPWVGRIPDIDLQQPMSEYVSNELKHAAILRKRIRELDGDPD
                     ALWNQPLKELKELWDFHASLPSLCELIASVQFGHEEFFPRTSQSFIERVAPIDPQTAA
                     IYRETLLADEAGHEWIAPEILTRYATDDLLQSRCLEALTKGCELFGKAIQSFNRSMPS
                     "
     gene            2912..3676
                     /locus_tag="VFY96_16455"
     CDS             2912..3676
                     /locus_tag="VFY96_16455"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608120.1"
                     /translation="MPTPALLMQVTDAKAFVDNLYKDVYSHWDKKINHGAFMTQLRAG
                     TLPLPALRRFFKDWGLFSIEVVALNAVSYHVHLPFFVENYDLLPAFSDKIAEELIAPK
                     PPGHILILLETAQSLRLSKEELFEQPASAAGRAISDYCRRVFQDGSLLELWATHAYEE
                     TLGHWAKQWGDALVTHYGMSKRQAIYFTAHAEADLVQHEDRMGHGPLNRMILQRILER
                     GLTAKKLGYAPKYCAYTMVDLHSLMEQNALENPYPS"
     gene            4008..>4270
                     /locus_tag="VFY96_16460"
     CDS             4008..>4270
                     /locus_tag="VFY96_16460"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEX5608121.1"
                     /translation="MVKLSTNIFVALIALVAFLSRNALAELPGHWQSRAPMPSARTEV
                     AAVELGGKIYVIGGYEKGGNLIEEYDPGTDRWRGRAPLPKPLH"
BASE COUNT         1028 a         1168 c         1121 g          953 t
ORIGIN      
        1 tgtccgacag ggctcgatcc cgaagctgtc tcacagcgct cgaaagatac tcgctagtgt
       61 cctcgaacta aaatgcattc gatcattcac gactcggatt cggaaatccg tagggacgta
      121 ggcatacgcc cattctggat cgggtaaagt ttacgcggcg gtagtaaccg ctaatagagg
      181 tttacccaat gattatcgat ggcgacgggc attttttcga gacggaagaa atatttgaca
      241 aatatatgga gccggcgctc cgaaactatc gcccgcgatt gttgaaagac gatcaaggcc
      301 acaatttctg ggtcgtggac gggcagaccc cctataaacg accatcgatc aaaggtgcgg
      361 gagctccggg cactgcggcg cctccggccg aggcgattca atcagcgcgg cgcgcttcac
      421 caggcagtca aaccttaacc aacctcaacg aaagactgaa agatctcgac agagaagaaa
      481 tcgatctgca gtttatttat ccaagctttc tcttgcacgt gaatgcctgg ctggacggca
      541 ttttagcgat gggagtttgc cgcgcgtaca acacctggtt agctgaaacc tgcaacagag
      601 cgccggaccg gctcagggcg gttggtgttg tttcgctgca agatccggcc ggagccgcca
      661 aagagctcga gcgcttgcgc gatttggctg tttccgcggt catgatcaac ggcaccgctg
      721 gggcgaaaag gctcgatcat cccgatcatg aagtgttttt tgccgaagca aatcgcttgc
      781 aaatgccgat cgccgtacat tttagcctgc agttccccgt ggtcgacaaa ctctttgagc
      841 atcatttccc caaccgtgtt ctcgcgggca ttttgccgat tatggcgggg ctcaccagcg
      901 tgctttgctc cggactgctc gaccgctaca aaaacctcaa atttgctttt ttggaaggag
      961 gcatcagctg ggtgcccgcc cacgtcgagc ggatggatga tcacttcgaa aacccgcgct
     1021 acggcgccag ggacctcatc agccatccac cgagcgatta tttgaaagac ggccggatct
     1081 tttttggttg cgaaggcaac gagtcgtttc tcagccgcat cctggacgaa gttggggaag
     1141 acctatttat attttcttcc gattaccctc acgccgaccg tactgaagga actgcaaaga
     1201 tcttgcgcga tcgcacggat attccgctga ccgtgaaaag caagctgctc gaacaaaacg
     1261 cccgccaatt ttatagcatt tgaaactcgt cgatcaggaa ttcttatgtc gggcgcttcc
     1321 gacggctatg caatcgctca atgaactatt tgaataccag caacaaaccg gcgtctaacc
     1381 atccgaaagg aggtgattct gtgaaaaatg cgatcagagc ttttattttg gtagcggtga
     1441 cgttcatgtt cagcgcggcc agcttcgccc aggccacgcc ggcaactcca gccacgccgg
     1501 caaccccggc caaacccgcg accccaccgg cgaccgaaaa gaagaatgaa acagcgacgg
     1561 aaaagtctag ctctacgagt gaggacaccg gcaagacgaa gtcaaaggcg gcgaaagcca
     1621 aaagcaagcg gacgaagaag gcgagcgaga aatcgacgga aaagatggaa gagaaaaaga
     1681 ccgacacgat ggaaaaaaag accaagtaat cttctctcga tcttccggcc caaagaggta
     1741 gccgacgagt tcggttacct ctttttgttt caaccgaagc atcgcgtgcg cgacaaaatg
     1801 ggcccgcccc tgtgcccaac ccttgcttga tttagcctat tccctcaact agactttttt
     1861 tagtggtggg ggaaacgatg ctgaacgcat tacaaggagg agcggagatg gacctgccga
     1921 agtgccaaaa atgcaacaac gggttactga ttccgctatc ggattacggg caggaagggg
     1981 cttcggtatt gtataaggct tgggcgtgca ccaacccgga ctgcgggttt tccatacgga
     2041 tagataaagg cgaggtcacg tacgggaaga gaatcgaacc caagcactag ctcattcaag
     2101 atcgatccgt acccccatca ctctaagttg ctcccgattc gctgttactt atgacggcat
     2161 gctccgattg aagctctgaa tcgccttgcc gaacaactca catcccttcg ttaaagcttc
     2221 cagacagcgc gattgcagca aatcatccgt ggcgtagcgg gttaggatct ctggtgcgat
     2281 ccattcgtga ccggcctcat ccgctaacaa agtttctcgg taaatcgccg cagtttgggg
     2341 atcaatcggc gcgacccgct cgataaatga ctgtgacgtc ctgggaaaaa attcctcatg
     2401 accaaattgg acgctcgcga tgagctcgca aagcgatggc aaagacgcat gaaaatccca
     2461 aagctctttc aattctttca gcggctgatt ccagagcgcg tcggggtcgc catcgagttc
     2521 cctgatccgc ttgcgcaaga tcgccgcatg tttcaactcg ttggacacgt actccgacat
     2581 gggctgctgc aggtcaatat cgggtatgcg tccgacccag ggcgcggtga cctccgcggc
     2641 tttgcgttcc atgacgacgc cgaactggag ccggcgcagc accaccgctg gcgtcagcgg
     2701 cttttcggct ccgtagctct ttcgctcggc attcatttgt tcccagaaag cttcccgggt
     2761 ggctgccaat tgattgataa actcattcgc ttttagtgcg gccatgcctc ctcccttgga
     2821 gtttcgcgca ttgacactgc aattaattta ggtattaaac aatacaggaa atccgctctg
     2881 tgtaaagatt caactggccc gcgactccga gttgcccacg cctgcactgc tgatgcaagt
     2941 caccgacgcc aaagccttcg tcgacaatct ttataaggat gtctattcgc attgggataa
     3001 aaagatcaat catggtgcgt ttatgactca actgcgcgcc ggcacgttgc cactgccagc
     3061 cctgcgccgc ttctttaaag actggggatt gttttcgatc gaagtggtcg cactgaacgc
     3121 tgtttcttac cacgtccatc tgccgttttt cgtcgaaaac tatgacttgc tgccggcgtt
     3181 ttccgacaag atcgccgagg aattgattgc tccaaaaccg ccggggcata ttttgattct
     3241 gcttgaaacc gcgcaatccc tacgcctctc gaaagaagaa ctcttcgaac agccggcatc
     3301 ggctgccggc cgggcgatca gcgattattg ccggcgagta ttccaggacg gatcgttact
     3361 tgagctttgg gcgactcatg cctatgaaga aaccctcggg cattgggcga aacaatgggg
     3421 cgacgcgctg gtcacccact acggcatgag caaacgtcag gcgatttatt tcacggctca
     3481 cgccgaagcg gacctcgtgc agcatgagga ccgcatgggt cacggcccgc tcaatcggat
     3541 gatcttgcag cgtatcctcg agcgcggctt gacggcaaaa aagctcggct acgctccaaa
     3601 atattgcgcc tataccatgg tcgacctgca ttcactcatg gaacaaaacg cgctggaaaa
     3661 tccgtatccc tcataacaac acagcggaga ttcttcgctg ccgccaccct gcgtgttgat
     3721 ggtcatctcg agctgaagcc gaaagatctt ctctttctat gtagctgaaa cgtaagatct
     3781 gctcaccgcg cgttcgagat gcgtcggttt gtccaacaca aagaatgaaa aatgagctgc
     3841 agtgcattcg gagcaacgcc caaatccccc aatggccccc tttttcaaag ggggtgggcc
     3901 gcaggacggg ggggtttttt ggtcctcaat ggtattttca taagagtctg ctgctgctcg
     3961 gaataacatc gagagccgtt gcgaaattcc cttggggaac aaggacaatg gtcaagctca
     4021 gtacaaatat ttttgtggca ttgattgcgt tagtggcttt tctgagccgc aacgcgctgg
     4081 cggaattacc aggtcattgg caaagccgtg cgcccatgcc atcggcccgc actgaagtcg
     4141 ctgccgttga gcttggtgga aaaatttatg tcatcggcgg ctacgaaaag ggcggcaacc
     4201 tcattgaaga atacgatccc ggcaccgacc gctggcgcgg ccgggctcct ttgccgaagc
     4261 cgttgcatca
//
LOCUS       DASULZ010000802         3978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_803315, whole genome shotgun sequence.
ACCESSION   DASULZ010000802 DASULZ010000000
VERSION     DASULZ010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803315"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..191)
                     /locus_tag="VFY96_16465"
     tRNA            complement(116..191)
                     /locus_tag="VFY96_16465"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(156..158),aa:Cys,seq:gca)
     gene            302..547
                     /locus_tag="VFY96_16470"
                     /pseudo
     CDS             302..547
                     /locus_tag="VFY96_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
     gene            1789..2262
                     /gene="rimI"
                     /locus_tag="VFY96_16475"
     CDS             1789..2262
                     /gene="rimI"
                     /locus_tag="VFY96_16475"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX5608122.1"
                     /translation="MASDDASFSSETLYFRPITTADLDELMVIERMSFRFPWSAGFFL
                     QELQVPCARSILAELGNRTVGYILFWLLPDAIDIHNIAVHLQYRRRGIARALLDEVIR
                     EARRRTFARVMLEVRRSNLPAQALYRRLGFTTTGIRKGYYSDDGEDALAMTLEIT"
     gene            2648..3130
                     /locus_tag="VFY96_16480"
     CDS             2648..3130
                     /locus_tag="VFY96_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX5608123.1"
                     /translation="MFKVGEKVVYPAHGVGEIEAIRSHVISGTEKKFYMLRILETDMK
                     IMIPIDNVESVGLRKIIDRAMVIKVYKVLRQKKVETDQQTWNRRYREYTEKIKTGSIL
                     EIAKVLRDLFVLKGDKELSFGERKMLDTARNLLVKELSIARSHSEEKIMEELRHIFTH
                     "
     gene            3199..3897
                     /gene="ispD"
                     /locus_tag="VFY96_16485"
     CDS             3199..3897
                     /gene="ispD"
                     /locus_tag="VFY96_16485"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX5608124.1"
                     /translation="MRISAIVLAAGEGRRLGGALPKAFAPIAGRPLVLRTLERILSVP
                     VIERVTLVVAAAQLERCEGLLKADPTLRARPWRLQAGGTTRQQSAKRGLEKAGADSDL
                     ILIHDAARPFVSAELIIRCIEAANRHGAAVPGLPAHDTIKVVAQDHWVERTLERASLW
                     EIQTPQVFRRELIVAAHENAASAGLDVSDDALAVERYGKPVFVVEGERLNFKITVPED
                     VWLAELLLREGRVS"
BASE COUNT          942 a         1057 c         1066 g          913 t
ORIGIN      
        1 cgaattgaaa gaggcaatca acgcggtttc gacgctgaag ttggaacaaa gccggaacct
       61 tttttagtga gaaggggaag aggactcgga ttctctaacg atatcacgcg tgaactggag
      121 gcggcgagcg gattcgaacc gctgcatcga ggttttgcag acctctccct taaccacttg
      181 ggtacgccgc cttgctccgt tagctaacat gaactttttg cgacttgcaa caatgtccga
      241 aattattgac aagacttagt gaaacacgta gttaaaaaaa gggcttacga ggagtttccc
      301 aatggcaacg atgattacca gcgattgcat taattgcggc gcatgcgagc ccgaatgccc
      361 gaataacgca atcagccagg gtgatcctgt ttacgtgatc gatccacagc tctgcaccga
      421 atgcgtcggg tttcacgatt acgaagcgtg cgctgcggtt tgcccggtcg atgtctgtgt
      481 gaccgatccg aacaatatcg agtccgaaga ggtattgatc gcgcgcgcgc gcgctttgca
      541 cccggaaacg gatttcggca acggttttca gtcgcgattc agaaaagctc aaggcgacgg
      601 cgctgcagcg gcaacggata aagccgccgt ggctgagccg aaagcggctc ctgttcagac
      661 tgccgcagca aaaccggaga gtacaccggc gagtccggcc tcatctgttg ctgtgccggt
      721 gcccgtggcc gctacggcag cgcccgccgt caaagcggct acggcagcgc ccgccgtcaa
      781 agcggctgcg ccagcggtgg ctcccaaacc cgtagcgcta aaaactcagc ccagaccgaa
      841 gaaaacattc gccaaggaat tgccaatcag cttcgaagaa gcagaaaaag aatatgccgg
      901 gcagcgcagc catctcacag gcggcgttcg gctggcggtt attctattac agccagtgat
      961 cggggcgttg ccccatggca ttaagaaacg gctcgagcta gcaatgcaga ctcccttgtt
     1021 cactgcggcc ggaagcaccg cagtcaacat cgttcacaac gcggtgctgt acccactggc
     1081 atttatcact gtagcagcgc tcatgaacgg accggaaatc attttcagcc aggatatcaa
     1141 caaatacatc ttaatcgggc tgttgcttgc tgggctggaa ggggtatttc gtttaaaaga
     1201 cggtattttt cgtgctaagc ctgcgacgga aatgaaattt ctcgcctcat tttacggagc
     1261 gccactgggc gttatcgctg caccattctt ggaacggtac actggcctgg tacgtgatat
     1321 tcccgttccg gtgcaaggat tctactctga aggattcgtc gataaggttg aaagagaacg
     1381 acgctacggc aatgtgtata ccgtcgcgga tcgcggtaag tcgtttctcg tgcggatgga
     1441 atttccccgg tatattcctg atatcggcat tgccgatcgc gagcgactgc ccgacgagat
     1501 gcccgattat gactatgacc tcgtgctggg cgatgaagag ttgatcgtca agggtaaatg
     1561 caccgatgaa aatgtgagaa aaatttcttc cagcatcggc gcattcccac cagaatttac
     1621 gacggtcatt tcactgcacg agaggatcac tgggtttgtc catcattttg acaataaact
     1681 tcttgaagtg gttctagtaa aagcttgagc tagctcgata tcgatttgag agacccaatc
     1741 gacaccaact ccaaatcagc cacaggccac tcggaagccc acgggcaagt ggcatcggat
     1801 gacgcctcat tctcgtccga gaccctctat tttagaccga tcacgaccgc cgatctcgac
     1861 gaactcatgg tgatcgagcg gatgtcattt cgttttccct ggagcgctgg tttttttctt
     1921 caagagctgc aggtgccctg cgcccggtcc attttagccg agcttgggaa ccggaccgtg
     1981 ggctatattc tgttctggct gctgcccgac gccattgaca ttcacaacat tgcagttcac
     2041 ttgcagtacc gccgacgtgg cattgcccgg gcgctgttgg atgaagtcat ccgcgaagcg
     2101 cgacgtcgaa catttgctcg agtaatgctt gaggttaggc gctcaaacct gccagcgcaa
     2161 gccctttacc gaaggcttgg gttcacaacg accggtattc gcaaaggcta ctattcagat
     2221 gacggtgaag atgctctagc aatgactctc gaaatcacgt aaatcgtcgg agcacaggaa
     2281 ggctacggct atcacttaca gcagacgtgg atatttcaca cgtaaatgat tgtaacccct
     2341 tttttttgcg gactccgtac cgcccttgac ggactctcct cctctagtat ataaattaat
     2401 tgttgcccga acgacattag cggtcagatc atcgaccgct aatttttttg actcgcgatt
     2461 agcttgggcg aagagagcgg catgctggag acccttcacc taaaccctca atttttatct
     2521 ttgccctgca gccgttttct ccaccatcgc tactcccgtc aaccaagccc ttcgatagtt
     2581 ttgctgattc gatccggcaa agcgctttta aaaacgctga cagtcttcag cctaggagga
     2641 gatccctatg tttaaggtag gagagaaggt tgtttacccc gcccacggag tgggtgagat
     2701 tgaagcgatt cgaagccacg tcatatcggg cacggaaaaa aagttttaca tgctacgaat
     2761 actcgaaacc gacatgaaaa taatgattcc gatcgacaat gtcgaatccg tcggtctccg
     2821 caaaattatc gaccgcgcca tggtcataaa agtctacaag gtcttgcgtc agaaaaaagt
     2881 cgaaacggat cagcagacct ggaaccgccg ctatcgcgaa tacaccgaaa aaatcaaaac
     2941 cggatcgatc ctcgagatcg caaaagtgtt gcgcgacctt ttcgtcctga agggcgacaa
     3001 ggaactttcg ttcggggagc ggaaaatgct cgatacggcg cgtaatctct tggtcaaaga
     3061 actttcaatc gcgcgctccc attccgagga aaagatcatg gaggagttgc ggcacatctt
     3121 tacgcactag attttcttgg tccctagatt actcaaaaga gccgagaaac cctctcggct
     3181 cttttgtttt gaggcatcat gcgcatcagt gccattgtcc tcgctgcggg agaagggcgc
     3241 cgtcttggcg gtgccctccc aaaagccttt gcgccgatcg ccggtcgtcc cctcgtactt
     3301 cgaactctcg agcgaattct ttcagttccg gtgatcgagc gagtcacgtt ggtcgtggcc
     3361 gcagctcagc tcgaacggtg cgaagggctg ctcaaagccg acccgacttt gcgggctcga
     3421 ccatggcgtt tacaggcggg cggtacgacg cgccagcaat cggccaagcg cggtttggaa
     3481 aaggccggcg ccgacagcga tctcatcttg atccacgatg cggcgcgacc gtttgtctct
     3541 gcggaactga tcattcgctg tatcgaagca gcgaaccgcc atggcgcggc ggtgcccgga
     3601 ttgccggcgc acgatacgat caaggtcgtc gctcaggatc attgggttga aagaacgctt
     3661 gaacgtgctt cgctgtggga aattcagacg ccgcaggttt tccggcgcga gctgatcgtt
     3721 gccgcccacg aaaatgcggc cagtgcgggg cttgatgtca gcgatgatgc gctcgcggtg
     3781 gagcggtacg gcaagccggt ttttgtcgtt gaaggcgagc gactgaattt taaaatcacc
     3841 gtgcccgaag atgtctggct cgctgaactt ttgttacgag aggggcgagt ttcctaggcg
     3901 gatttgtttc gcaatacctc attgaggctg ccgctacgaa aaccttgcag gtcgacgctc
     3961 acgtaattga aacccact
//
LOCUS       DASULZ010000803         4323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_94016, whole genome shotgun sequence.
ACCESSION   DASULZ010000803 DASULZ010000000
VERSION     DASULZ010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94016"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VFY96_16490"
     CDS             <1..556
                     /locus_tag="VFY96_16490"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX5608125.1"
                     /translation="GRSRRTVRSKTLEQLKRLDVGGWRGRAFAGERILTLEEALDSID
                     DHADLCLEIKSYQGSAPGIELKLLFIVSHYDYFERTTFSSFDYGSLKRLRELAPDANI
                     GLIVGSPLKPNWLEAARAVTAKSLHVQKELATRELFSQAWEEGLDVFVWTVNELHEME
                     TLGGMGVQGLISDFPEKFSKLCCR"
     gene            719..913
                     /gene="rpsU"
                     /locus_tag="VFY96_16495"
     CDS             719..913
                     /gene="rpsU"
                     /locus_tag="VFY96_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX5608126.1"
                     /translation="MTGVRVRENESIESAIRRFKKLCEKAGILAELRKREHYEKPSVK
                     KKKKAIAAKKRAMRRVRHSF"
     gene            934..1380
                     /locus_tag="VFY96_16500"
     CDS             934..1380
                     /locus_tag="VFY96_16500"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX5608127.1"
                     /translation="MELQNEIKEAVKSAMKSGDAVTLSTLRLLLAAIHNEEIKFQREL
                     RLEEIHRITATLCKQRGEAIDLYRKGNRADLARKEEAELEVLKRWLPQPLSDEEVKNI
                     IQASIAEAGARGVQDLGKVMKLVMPKVSGRSDGKQVSELAKTLLAG"
     gene            1476..2060
                     /locus_tag="VFY96_16505"
     CDS             1476..2060
                     /locus_tag="VFY96_16505"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX5608128.1"
                     /translation="MARERECGNSGTRPQWFRAVNWIDVILACVLALFGLRGFFRGFF
                     REIFSLLGLIAGFILATEWAQPAASYAAQWWKLPPLFLKGAAFIAIFFVAYFACNLIG
                     WLLHRSESLLFIKSLNRAGGLLVGLGKGAAILALVVFFLAGAGSVSKANTENSHDSYL
                     VPPLSRFAEGIIRLGKQQLLTRLNWGEAAPTHRL"
     gene            2068..3861
                     /gene="dnaG"
                     /locus_tag="VFY96_16510"
     CDS             2068..3861
                     /gene="dnaG"
                     /locus_tag="VFY96_16510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX5608129.1"
                     /translation="MISPEKISEIRHRASIVEVISDYVTLKKAGRNHMGLCPFHGEKT
                     PSFTVSEEKGIYHCFGCQAGGSVFNFLMQYDHLAFPEAVERVAKRYGIMIERSEQSAA
                     ARAAGDREYLYRINEQAAAHYQKILFRHPEGRKALEYLTSRGVDEAAARKFMLGYAPQ
                     GGSGLLQLLRQESIAIQDALRLGLIGERAPRQYREKFFARLIFPIANAAGKIVAFGGR
                     VLDNSLPKYLNSSETPLFHKRSTLYGLHQAKEGIRKADRVVVVEGYLDVIALAQHELS
                     YAVATLGTALTVDHIRLLSRYTKNVIALFDGDDAGQKAAARSFEVFVEAGLLGRAAFL
                     PKQDDPDTFVRKHGKARLEAILDQAVPLADFFFLWLERRYGKSLEGKSQIAAEVSRVL
                     TKLANPLEVDLLVGRAVDLLGIREETLRQRAHSPLPTRTAHNRPVAPSPMPREDKAER
                     MLIGAMLRLPKVIEQVAVDDEARQGFSSLWAPAVDAILNQWQNHQNTEVAQLTETLPA
                     ELSSEIAGLALETEDLTDAVCGKVAADCLARLKRKHIKAQKRDERLKIRVAEEQKDEN
                     AKRERILEWQDLVRKERQLEHRKLEPKARVR"
     gene            3915..>4323
                     /locus_tag="VFY96_16515"
     CDS             3915..>4323
                     /locus_tag="VFY96_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HEX5608130.1"
                     /translation="MKEVKKLIDLGKEKGYLTYDDVNDMLPAEVVSPDQIDDVMSIFG
                     EMDIEVVDANQRVTLGGVGEELAEEEEEEKEIELDGDGDVVGKTGDPVRMYLREMGTV
                     SLLSREGEVEIAKKIEEGEKRVIHEVLSSPLALT"
BASE COUNT         1022 a         1123 c         1269 g          909 t
ORIGIN      
        1 tgggcgcagc aggcgaacgg ttcgtagcaa aaccctcgag caactcaaac gactcgatgt
       61 gggcggatgg cggggacgag cttttgcggg cgagcggatc cttacactcg aggaggcgct
      121 cgactcgatt gacgaccacg ctgatctttg cttggaaata aaatcctatc agggttcagc
      181 gccaggcata gagctcaaac ttttattcat cgtgagtcac tatgactact ttgagcgcac
      241 gacgttttct tccttcgact acggctcgct gaagcgcttg cgggagttgg cgccggacgc
      301 gaacatcgga ctcatcgtcg gttcaccgct gaagccgaat tggctcgaag cggcacgagc
      361 cgtaacggca aaatcacttc acgtgcaaaa ggaactcgca acgcgggagc ttttctcgca
      421 agcatgggaa gagggactcg atgtctttgt gtggacagtg aacgaacttc acgagatgga
      481 aacactgggc gggatgggcg tgcaagggtt gatttccgat tttcccgaaa aattttccaa
      541 gctgtgttgc agataacaga cgtagggcga ctcctgaatg tctcagcgag cgatccagtt
      601 gcctccaaac cgttcatttt taaggcaaat tgcttgactt ggaagggaaa tgcgctagaa
      661 ttaccgccgg gcaaaacaca cggcacgatc cgtcggcgat cgggggtgat gtaagtttat
      721 gaccggagtt cgagtgcgag aaaacgaatc catcgagagc gccattcgac gcttcaaaaa
      781 attgtgtgag aaagcaggta ttttggccga actgcgcaaa cgcgagcact acgaaaaacc
      841 aagcgtgaaa aagaaaaaga aggcgatcgc ggcgaaaaag cgcgcgatgc gccgcgtgcg
      901 tcattccttt taacgtagcg taaatgtcgt ttcgtggaac ttcagaatga aataaaagaa
      961 gcggttaaga gcgccatgaa gagcggcgac gccgtgacgc tctcaaccct tcgtcttctt
     1021 ttggccgcca ttcacaacga ggaaatcaaa tttcagaggg agcttcgcct cgaggaaatt
     1081 caccgcatca ctgcaactct ttgcaagcaa agaggtgaag cgatcgattt ataccgcaaa
     1141 ggcaaccgcg ccgatctggc tcgcaaagaa gaagccgagc tggaagtttt aaagcgctgg
     1201 ctgcctcagc ctctttccga cgaggaagtg aagaatatca ttcaggccag tatcgccgaa
     1261 gccggcgcgc ggggagttca ggacctcggt aaagtcatga aactggtgat gccgaaggtc
     1321 tctggcagga gcgacggcaa gcaagtgagc gagttggcga aaacgcttct ggctgggtaa
     1381 ggagagcgcc agccagcggt tgcgtttgcc gcaattcaaa atggctcgat tgactttccg
     1441 agacgtgtgc agagcagaat aattccgcgc gcgttttggc gcgcgaacgg gagtgcggca
     1501 attccggcac gcgccctcaa tggttccggg ctgtgaactg gatcgacgtg attcttgcct
     1561 gcgtcctggc tctcttcggt ctgcgagggt ttttccgcgg tttttttcgc gaaatttttt
     1621 ccctgttggg cttgatcgcg ggttttatac tcgcaacgga gtgggctcaa cccgcggcga
     1681 gttacgccgc gcagtggtgg aagttgccgc cgctgtttct gaaaggcgcc gcattcatcg
     1741 cgattttttt cgtcgcatac tttgcctgca atttgatcgg ttggctgctg catcgctcgg
     1801 aaagtctgtt gtttatcaaa tcactcaatc gcgccggagg tctgcttgtg ggattgggaa
     1861 agggtgcggc gatcctggcg cttgtggttt tctttttggc tggcgccggt tcagtttcca
     1921 aggcgaacac ggaaaattct cacgattcgt atctggtgcc gccgctgtcg aggtttgccg
     1981 aagggattat ccgtcttggc aagcaacagc tgctaacccg cctgaattgg ggtgaggcgg
     2041 ctcccaccca caggctctaa tcttctcatg atcagcccag aaaagatcag cgagattcgt
     2101 caccgcgctt cgattgtcga agtgatctcc gattacgtca cgctaaaaaa agccgggcga
     2161 aaccatatgg ggctttgtcc tttccacggc gaaaaaaccc cctctttcac ggtgagcgag
     2221 gaaaagggca tctatcattg tttcggctgt caggcgggtg gcagtgtctt taattttctg
     2281 atgcagtacg atcatttggc ttttcccgaa gcggtggaac gtgtcgcgaa gcgctacggg
     2341 atcatgattg aacggagcga gcaatctgcc gcggcgcgcg ccgcgggaga tcgcgagtat
     2401 ctctatcgca tcaacgaaca ggctgcggcg cactatcaaa aaattctgtt tcgccatccc
     2461 gaaggccgga aggcgctcga gtatctgact tcccggggcg tcgacgaagc cgctgcgcgc
     2521 aaatttatgc tcggctatgc gccgcagggc ggctcgggac tcttgcagtt gctgcgccag
     2581 gaaagtatcg ccattcaaga cgcgcttcgg ctcggcttga tcggagagcg ggcaccgcgg
     2641 cagtaccggg aaaagttctt cgcgcggctg atctttccga ttgccaacgc cgctgggaaa
     2701 atcgtcgcct tcggcggtcg agtgctggac aactctctgc ctaaatacct caattccagc
     2761 gaaacgccgc tgttccataa acgatcaact ctttatgggc tgcaccaggc gaaagagggt
     2821 attcgtaagg ctgaccgcgt cgtggtggtc gaaggttatc tcgatgtgat cgcgctcgcg
     2881 cagcacgagc tttcctatgc ggtcgcgacc cttggcactg cattgaccgt cgatcacatt
     2941 cgcttgttgt cacgttacac taaaaatgtg atcgccctgt ttgacggaga tgacgccgga
     3001 cagaaggccg ctgcgcggag cttcgaggta ttcgtcgaag caggactgct cggccgggca
     3061 gcgttcttgc ccaagcagga tgatcccgac acgttcgtgc gaaaacacgg aaaagcacgt
     3121 cttgaagcga ttctcgatca ggcggtgccg ctcgcggatt tctttttttt gtggctcgag
     3181 cggcgctacg gtaaaagcct cgaaggcaaa agccagatcg ctgctgaagt cagccgggtg
     3241 ctgaccaaat tggctaatcc gctcgaagtc gatctgctcg tcggccgtgc cgtcgacctg
     3301 ctcggcattc gtgaagaaac acttcgacag cgggcccact cgcctctgcc cacacgcaca
     3361 gcgcacaatc gtccggttgc accttcaccg atgccgcgcg aagacaaagc ggagcggatg
     3421 ctgatcgggg cgatgctgcg cttacccaaa gtcatagaac aagttgcagt ggatgacgaa
     3481 gcgcgtcagg gattttcatc attgtgggct ccggcagttg acgctatcct caaccagtgg
     3541 caaaaccatc agaataccga agtagcccaa ttgactgaaa ccttgccggc ggaactctcg
     3601 agtgagatcg ctggtttagc cctggaaaca gaggatctca ccgatgccgt ttgcggcaaa
     3661 gtcgcggcgg actgcctggc gcgtttgaag cgcaagcaca tcaaggcaca aaaacgggac
     3721 gagcggctca aaatccgcgt cgcggaagaa cagaaagatg agaacgcgaa gagagaaagg
     3781 atactagaat ggcaagacct cgtacgaaaa gaacgccagc tagaacatcg aaagctcgaa
     3841 ccgaaagcgc gcgtccgata gccaagactc atgccgcctc cgactctgcc gcccagccgg
     3901 tggagaagaa aaatatgaag gaggtcaaaa agctcatcga tctgggcaaa gaaaagggct
     3961 acctgactta tgacgatgtc aacgacatgc tcccggcgga agtggtctcg cccgaccaga
     4021 ttgacgacgt tatgtcgatt ttcggcgaga tggacatcga agtcgtcgat gccaatcagc
     4081 gcgtgaccct gggcggcgtc ggcgaggagc tcgcggaaga agaagaggag gaaaaagaaa
     4141 ttgaactcga cggcgacggc gatgtcgtcg gcaagacggg cgatcccgtg cgcatgtacc
     4201 tgcgcgaaat gggaaccgtc tctctcctta gccgcgaggg cgaggtcgag atcgccaaga
     4261 aaatcgaaga aggcgagaaa cgagtcatcc acgaagtttt gtccagcccg ttggcgctaa
     4321 ctt
//
LOCUS       DASULZ010000804         2142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_94237, whole genome shotgun sequence.
ACCESSION   DASULZ010000804 DASULZ010000000
VERSION     DASULZ010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94237"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /gene="argF"
                     /locus_tag="VFY96_16520"
     CDS             <1..919
                     /gene="argF"
                     /locus_tag="VFY96_16520"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX5608131.1"
                     /translation="LAHELKRKQRRGVIHHLLTGKSLALVFDKPSLRTRATFEIGMNQ
                     LGGNVFFMGGAEVGLGTRETPADCARSLARWVDLIAVRTFTQATLEEMAAYSSVPVIN
                     ALTDLYHPCQVLADCQTLVEHKGKLDGLKITFVGDGNNMVHSWLEAAALVPFSFTLAC
                     PKGYEPDSEIFAKARAAGADVNVTQSVAEAVSGADAIYTDVWTSMGQEEEAQRRRRVF
                     RDYQVNSKVVRMAQSDALVMHCLPAHRGEEITDEVLESPQCVAFDQAENRLHVQKAVM
                     VWLLNPLKNAKVQSSISFGRVLSKKKSRI"
     gene            927..2132
                     /locus_tag="VFY96_16525"
     CDS             927..2132
                     /locus_tag="VFY96_16525"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX5608132.1"
                     /translation="MNVKKVVLAYSGGLDTSVILRWLKEQYGCEVIAFCADLGQGEDL
                     EALKRKALKTGASKVVIGDLREEFVKNYVFPMLRANAVYEGSYLLGTSIARPLIAKGQ
                     IDVAGKEKADAVSHGATGKGNDQVRFELTYYALKPGIQVIAPWRLWDLNARSTLIEYA
                     RKHGIPVPVTKAKPYSTDRNLFHISYEGGILEDPWKEPPADMFVWCRPLEKAPNRPEY
                     IEIEYLKGDPIAVNGRKMSAAKLLASLNDIGAKHGIGRVDIVENRYVGMKSRGVYETP
                     GGTILHAAHRGLESITMDREVLRLRDSLMPRFAEMIYYGYWFAPEREVLQKAIDASQA
                     NVSGHVRLKLYKGNVMVVGRRSDLSLYDPNVATFEADQVYRQADADGFIRLNALRLRL
                     QQLRTAKQR"
BASE COUNT          484 a          549 c          653 g          456 t
ORIGIN      
        1 cctcgcccac gagttgaaac gcaagcagag acgcggcgtc attcatcact tactgacagg
       61 aaagagcttg gcgttagtct ttgataagcc gagtttgcgc acgcgcgcaa ctttcgagat
      121 cggcatgaat cagttgggcg ggaatgtttt ttttatgggg ggtgccgagg ttgggcttgg
      181 aacgcgcgaa acgccggcgg attgcgcgcg cagcctcgcg cgttgggttg atctcattgc
      241 cgtgcggacc tttacccagg ctacactgga agagatggcg gcatactcct cggttccggt
      301 gattaacgcg ttgacggatc tttatcatcc ttgtcaggtg ctcgcagatt gccagacgct
      361 ggtagagcat aaaggcaagc ttgatggatt gaagattacg tttgttggcg acggcaacaa
      421 tatggtccat tcctggctcg aagccgcggc attggtgcca ttttcgttca ccctggcctg
      481 tccgaaaggc tacgagccgg atagtgaaat ctttgccaag gcgcgagccg cgggcgcaga
      541 cgtaaatgtc acgcaatcgg tcgcggaagc ggtcagcgga gcggatgcca tttacaccga
      601 tgtttggacc agcatgggcc aggaagagga ggcccagcgg cgccggcgcg tttttcgaga
      661 ctaccaggtc aatagcaaag tagtcaggat ggcgcagagt gatgcgctcg tgatgcattg
      721 cctgccggcg catcgcggtg aggaaattac ggacgaggtc ctggagagtc cgcagtgcgt
      781 ggcctttgac caagcggaga atcgtctgca tgtacagaaa gccgttatgg tgtggctcct
      841 caaccccttg aagaatgcca aagtgcagag ctcaatttca tttggcagag tgctgtcgaa
      901 gaaaaaatcc agaatttagc aactcgatga acgtcaaaaa agtggttctt gcctattccg
      961 gcggattgga cacttcggtg atcctgcgct ggctcaagga gcaatacggc tgtgaagtga
     1021 tcgccttttg cgccgacctc ggccaaggcg aagatcttga agcgcttaaa cggaaggcgc
     1081 tcaaaacggg cgccagcaaa gtcgtcattg gcgacctgcg cgaggaattc gtcaagaact
     1141 atgtttttcc tatgctgcgc gctaatgcgg tctatgaagg ctcttatttg ctgggcacct
     1201 cgatcgcgcg acctctcatt gcgaagggac agatcgatgt ggccggcaaa gaaaaagccg
     1261 acgcggtgtc gcacggggct accggcaaag gcaacgatca ggtgcgtttc gagctcactt
     1321 actatgcctt gaagccggga attcaagtca tcgcgccgtg gcggttatgg gatctgaacg
     1381 cgcggtcgac actgatcgag tatgcgcgca aacacggcat tcccgtgcct gttacgaaag
     1441 ccaaacccta cagcacggat cggaatctgt ttcacatcag ctatgaaggt ggaattctcg
     1501 aagatccatg gaaggagccg ccggcggaca tgttcgtctg gtgccggccg ctcgagaaag
     1561 cgccgaaccg gcccgagtac atcgagatcg agtatttgaa gggcgatccg atcgcggtca
     1621 atggccgaaa aatgtcggcg gctaagttat tggcatccct gaacgacatc ggcgcgaaac
     1681 acgggattgg ccgagtcgat atcgtagaga accgttatgt cggcatgaaa tcccgcgggg
     1741 tttacgagac cccggggggc acgattctgc acgcggcgca tcgcggactc gaatcgatca
     1801 ccatggatcg cgaagtgctt cgcctcaggg acagcttgat gccgcgtttt gccgaaatga
     1861 tctactacgg ctattggttc gcaccggagc gcgaggtatt gcaaaaggcg atcgacgcgt
     1921 cccaagcgaa cgtcagcggt catgttcggc tgaagctgta taaaggaaac gttatggtcg
     1981 tcgggcgcag gtccgatctt tccctctatg atccgaacgt cgccaccttt gaagcggacc
     2041 aggtttatcg ccaggccgac gccgacggtt tcattcgatt gaacgccttg cggttgcgct
     2101 tacagcagct gcggaccgcc aaacagcgat agttatggcc ga
//
LOCUS       DASULZ010000805         2537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_296245, whole genome shotgun sequence.
ACCESSION   DASULZ010000805 DASULZ010000000
VERSION     DASULZ010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296245"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..123
                     /locus_tag="VFY96_16530"
     CDS             <1..123
                     /locus_tag="VFY96_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX5608133.1"
                     /translation="APVARVCSLEVPMPYPKHLEDAAIPQVPEIVAEARRVVTL"
     gene            584..1471
                     /locus_tag="VFY96_16535"
     CDS             584..1471
                     /locus_tag="VFY96_16535"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5608134.1"
                     /translation="MFTRILVPLDGSANAERVLPYARALARTLEIPVTLITVVETAAD
                     GFPKKVGDLDTRIQESMKSSEEYLEKISRTFAEASVRYTVEKGKAEDAIIINAAADNR
                     TLITMASHGRTGVGRWLLGSVAEKVVHGANNAVLVVRADQEMTSKGEAAPDSIIVPLD
                     GSNQAETVLLYVVELARAFHVKVTLLRAYSLKQIIFSFEQYHPDLDGLKDALRWEAKT
                     YLDEKVAQLKSRGLVDVFCFATEGDAPETIIEMGKGAPRTLIAMSTHGGSMIKHWVLG
                     SVTEKVLRHANNAVLIIRT"
     gene            1675..2124
                     /locus_tag="VFY96_16540"
     CDS             1675..2124
                     /locus_tag="VFY96_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5608135.1"
                     /translation="MTPRIPESLKWEHEELHAELARATKAGGKTGEAAKAVAGVLHNH
                     FLKEEEFALPPIGLLAALARGEIEDEMRDVLVMTERLKAELPEMLREHQAVVTALAEL
                     TAAAKKEKLAEHARFAKKLMVHAQTEEEVLYPAAILIGEYLKLRLKA"
     gene            2178..>2537
                     /locus_tag="VFY96_16545"
     CDS             2178..>2537
                     /locus_tag="VFY96_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX5608136.1"
                     /translation="MPGKIHCYLAADHDRLDALLERAISNPANIDAAAYAQFRAGLLK
                     HIAMEEKVLLPAAQKARGGDPLPIAARLRLDHGALVALLVPSPTARIVAAIRAILKVH
                     NPIEEDPGGVYDECEKLA"
BASE COUNT          638 a          654 c          725 g          520 t
ORIGIN      
        1 gctcctgtgg cgcgggtgtg tagcctggaa gtgccgatgc cctatccgaa acatcttgaa
       61 gatgcggcga ttccacaggt gccagagatc gtcgccgagg cgcgccgggt agtgacccta
      121 tgagtagtga attccgccta cacggcaccg cggaaaaacc aactgagcca tgaaatgcgc
      181 ggtgaaagta gacgtttcgg tcgtttcgcc gcactttctc ggctttgccg cgatcgggac
      241 tgttctcctt gtcttccgca agaccatagg agccaatggg gtgacgcgat tagatgatcc
      301 gtttttttgc tgaggactgc gcgaacgaga aatcgcacca tgtcattatc gagctgcaga
      361 atatcaagaa gttactaaga aagtcgatat cgaactactg ttgtgatccg gcgaactcga
      421 aaactctatc gagcgctcct ttagggttct ggcgacctct ttgcagtgct ttggatcgcg
      481 ctcgcgctaa actccaggta ccgcgcagac tggtcctcga taatttctcg attgcgacca
      541 ctacgcgatt gcgattgggt ctttcataga tcaggagggc aagatgttta cccggatact
      601 ggtgccgctg gacggatccg ccaacgcgga aagagttctg ccgtacgccc gcgcgttggc
      661 acgaaccctc gaaataccgg taaccctgat aacggttgtc gagacagccg ccgacggttt
      721 ccctaaaaaa gtcggtgatc tcgatacgcg catacaagag agcatgaaaa gcagtgagga
      781 atatctcgag aaaatatccc ggacattcgc cgaggcgagc gtccgataca cggtggaaaa
      841 aggcaaagcc gaagacgcta ttatcataaa tgccgctgcg gataatcgaa cgctgatcac
      901 gatggcttcc catgggcgta ccggagtggg ccgctggctg ctcggcagcg tcgccgagaa
      961 agttgtgcat ggagcgaata acgctgttct tgtagtgaga gcggaccagg aaatgacttc
     1021 caagggtgaa gcagctccgg attcaatcat tgtcccgctc gacggttcga accaggccga
     1081 aaccgttctc ttatatgtcg tcgaattggc aagagctttc catgtgaagg tcacgctctt
     1141 acgcgcatac agcctgaaac agatcatctt cagcttcgag caatatcatc ccgatctgga
     1201 tggactcaaa gacgcattga gatgggaagc caaaacttat ctggatgaaa aggtagcgca
     1261 actgaagagt cgcggtctag tggatgtgtt ctgtttcgcc accgaaggag acgctccgga
     1321 aacaatcatc gaaatgggta agggagcacc gcgcacgctg attgcaatgt ccacccacgg
     1381 cggctcgatg atcaagcatt gggtacttgg aagcgtcacc gaaaaagtgc tccgtcacgc
     1441 caacaacgcg gtattaatca ttcgcacctg atcccgtgcg cgaagcgcgt tagcggcgcg
     1501 agactcatta gcgtattgtt tcgccgcaga gcaggtggga gtacgtaaag agctgagtgg
     1561 agtttattca cgatgtgaga aaagctacga ggcggcaaaa actttgccga cggcaattat
     1621 tctgaaccca aggagctttg tataaagagt agaataaacg aaggaggtgc aaatatgact
     1681 cccagaattc cggaatccct gaaatgggag catgaggagc tccacgcgga gcttgcgcgg
     1741 gcgacgaaag cgggcggcaa aaccggcgaa gcggcgaaag ccgtagccgg agttctgcat
     1801 aaccacttcc tcaaggagga agaatttgct cttccaccga tcggtctatt agcggcgctg
     1861 gcgcgcggcg aaatcgagga tgaaatgcgc gacgttttag tcatgaccga gcggctcaaa
     1921 gcggagctgc cggaaatgct tcgcgagcat caagcggtgg tgacggcgct ggcagaattg
     1981 accgccgcag cgaaaaaaga aaaactggcg gagcatgcgc gctttgcgaa aaagttgatg
     2041 gtacacgcgc aaacggaaga agaggttctc tatccagccg ccatattgat cggcgaatac
     2101 ctgaaactca gactgaaggc ctaacaaacc agcgctgctc gacagcattt taagcagcac
     2161 gccgaaagat gcttcctatg ccaggaaaaa ttcactgcta ccttgccgct gatcacgatc
     2221 gtctcgatgc tctgctcgaa cgcgccattt ctaacccggc gaatatcgat gccgcggctt
     2281 acgcccaatt tcgggccggt ctactgaaac atatcgcgat ggaggaaaag gttcttctgc
     2341 ccgcggcgca aaaagcgcgc ggcggcgacc cgcttccgat cgcggccagg ctaaggctcg
     2401 accacggtgc gttggttgcg ctgctcgtac cgtcgccgac tgcacggatt gttgcagcca
     2461 ttcgtgcgat tttgaaagtc cataatccga tcgaagagga tcccggcggg gtctacgatg
     2521 agtgcgaaaa gctggcc
//
LOCUS       DASULZ010000806         4301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_397251, whole genome shotgun sequence.
ACCESSION   DASULZ010000806 DASULZ010000000
VERSION     DASULZ010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397251"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..384)
                     /gene="crcB"
                     /locus_tag="VFY96_16550"
     CDS             complement(1..384)
                     /gene="crcB"
                     /locus_tag="VFY96_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX5608137.1"
                     /translation="MLGIILVGIGSFFGGALRYLVSTWVYRVLDSPWFPYGTLIVNVS
                     GCFVIGFLAGLAESRAAFTSEMRLFVFVGVLGGFTTFSSFALETLSLVRDTEQMAALA
                     NIGLQFLLGLLAVWVGNMLAHAIGG"
     regulatory      complement(425..493)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            548..1417
                     /locus_tag="VFY96_16555"
     CDS             548..1417
                     /locus_tag="VFY96_16555"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017849113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-polyphosphate phosphotransferase"
                     /protein_id="HEX5608138.1"
                     /translation="MKIDPDDFRVKDGKKVNLKKWPTELKAYYETKKQFKQALQEHVD
                     ELSELQSLLYASNRFAILLIFQAMDAAGKDSAIKHVMSGVNPQGCQVFSFKHPSAEEL
                     DHDFLWRTTRNLPERGRIGIFNRSYYEEVLIVRVHKELLRAENLPQQILDDKDIWDER
                     YRSIRDFEAHLDRNGTRIVKFFLHVSQEEQRKRFLARIDEPEKNWKFNRGDIEERKYW
                     RDYMAAYEACLEATSTRHSPWFIVPADDKQNARLIISKIIVATLRKLKMQYPVLDEKR
                     RSELRSLRKLLEK"
     gene            complement(1585..1878)
                     /locus_tag="VFY96_16560"
     CDS             complement(1585..1878)
                     /locus_tag="VFY96_16560"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEX5608139.1"
                     /translation="MSIVRVIYVTVKPEQVKDAINLWKNHCAPLMIKQPGCLSEKLLE
                     CIDAPGEFISYSEWDTQASIDRYRDSEAHAEIQRHARGLQGGGRTSVKRYDVK"
     gene            2332..2808
                     /locus_tag="VFY96_16565"
     CDS             2332..2808
                     /locus_tag="VFY96_16565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608140.1"
                     /translation="MFVENYRQDNVDSLMGGNNASPIPYPKISDEDWRVWNLFLPVRA
                     VNLDRIAASNARQGMMLSLYDIPYAVTDEIRKANPYFERIEVWRKHEIEKDPIAVGIA
                     GGQRYMIARWGVDKLIPFESIKKSVPLILAWQFISSPLAAMSGIAALGLVAWSFLV"
     gene            3112..4209
                     /locus_tag="VFY96_16570"
     CDS             3112..4209
                     /locus_tag="VFY96_16570"
                     /inference="COORDINATES: protein motif:HMM:NF016999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEX5608141.1"
                     /translation="MNEFKYLQTRMPGIVRALAIGIPAGFLFEFLRTPIPWMIGPMIG
                     VASLNLMGVQMHSPPYARQMGQVILGSAVSLYFTPPVVAALGGNWPAIAATTVTVFLV
                     GIFGALTLSRASGVDAKSTFFASIPGGAMAMAVLADRYGAQIAPVAVAHSLRVSLVVI
                     LIPFALTYGGFPLEAAAYRPALPLSIPILAFWLAVGWLLGEVSEKFHFHNGCLLVPIF
                     LGAALTMGGVQLSSVPRWMTDFAQLMFGLVLGARYERAFFTRYRLFIPFALLNSFFIL
                     IASLLAGAALAWAFSLPVATMILATSPGGLAEMTITAQALHLSVPLVVAFHLFRVVIV
                     NIGTQYIYVWTAGLFNRAPTAVRKTAPSIGS"
BASE COUNT          957 a         1197 c         1128 g         1019 t
ORIGIN      
        1 ttatcctcct atcgcatgcg cgagcatgtt ccccacccat acagctaaaa ggcccaataa
       61 aaactgcaag cctatattgg caagagccgc catctgctcg gtatcacgta ccaaggaaag
      121 agtttccagc gcaaaggagg aaaaggtcgt aaaaccgccg agaacaccga caaaaacgaa
      181 gagtcgcatt tccgaggtaa acgctgcccg gctttccgcc aaccccgcaa gaaagccgat
      241 cacgaagcag cccgaaacgt tgacaatcaa cgttccatag ggaaaccacg ggctatccaa
      301 cacacgatac acccaggtac tgaccaggta ccgcagcgcc ccaccgaaga aacttccgat
      361 tccgaccagg atgattccga gcatgctttg tgatccaccc aaaaaaagac ccctacttcg
      421 ggtttgagaa gtaggggtca tcaattcgct ggcgcgaatg gtttgactgg cgaaccccat
      481 cgcctttagt ttggttatta tttgtacaac cggtgccaca agtcaaacgt catcatgaaa
      541 ggaccctatg aaaatcgatc ccgacgattt ccgcgtaaaa gacggcaaga aagtcaacct
      601 gaaaaaatgg ccgacggaat taaaggcgta ctacgagacc aaaaagcagt tcaaacaggc
      661 gcttcaggag cacgtcgacg agctgagcga actgcaaagc ctgctatatg cctcgaatcg
      721 tttcgccatc ctcttaatct tccaggcgat ggatgccgcc ggcaaggata gcgcgatcaa
      781 gcatgtgatg tccggggtca atcctcaggg ctgccaggtt tttagtttca agcacccgag
      841 cgcagaggaa ctggatcacg acttcctatg gcgcaccact cgaaaccttc cggaacgcgg
      901 gcgtatcggt atcttcaacc ggtcgtatta cgaagaggtc ctgatcgtcc gggtgcacaa
      961 agagctcctg cgcgcggaaa acctgccgca gcagattctg gacgataagg atatctggga
     1021 cgagcgctac cgatcgattc gcgatttcga agcacatctc gatcggaacg gcactcgaat
     1081 tgtcaaattt ttcctccatg tttcgcagga agaacagcgc aagcgctttc tcgcacggat
     1141 cgatgaaccg gaaaaaaact ggaagtttaa tcgtggtgat atcgaagaaa ggaaatactg
     1201 gcgcgactac atggcggctt acgaagcatg tctcgaggcc accagcacgc gtcactcgcc
     1261 atggttcatc gttcctgcgg acgacaagca gaacgcgcgc ttgatcattt cgaagatcat
     1321 cgtggctacg ctgcgcaagc tgaaaatgca gtacccggtc ttagatgaaa aacggcgtag
     1381 cgagctccgc tcgctccgta agctgctgga aaaatgattc gaagccgcac cccggttggt
     1441 aggcccggag tgacgagcag ccatcgcaat cctcgcgaca gcgccggcgc tctaggccaa
     1501 ccaaggtggg aaccgcttta ctgggcgtct tctcgcaacg acccgactgc agcgcaggcg
     1561 tgctgagtga cacggcggct acaattactt cacgtcgtag cgcttgacgc tcgtcctgcc
     1621 gccaccctgc aatccccgcg catgcctctg aatctcggca tgagcttcac tgtctcggta
     1681 gcggtcgatg ctcgcctgag tgtcccactc ggaataggaa atgaattcgc cgggcgcatc
     1741 gatgcattct aggagttttt ccgataggca tcccggctgt ttgatcatca gcggagcgca
     1801 atgatttttc cacaggttga tagcatcctt gacctgttcc ggtttgacgg tgacgtaaat
     1861 gactctgaca atcgacatgg gacattacct cctatgaatt gatttcggcc agtgactcgc
     1921 ccgctgcttt cactggaagc acaaaaaggg ccgtattcaa cgccaccgcc agtttaaaat
     1981 ttttacctgc gcaaccgatt tctcccgtga acaaagcgcg ccgccgcaag cggccgctgt
     2041 tttgtttgct ctagttactg ttgtgtatgt ggcgtttttc atactacaaa cgagctctgc
     2101 gtcagaaaaa aaggacaaac cttcttcggc tcagcagctt tccacgagct aacttgagaa
     2161 atattccacg tatgtgccga gtaaaatacc ggcccgtttt ttgcctcgca cgtttgttat
     2221 tccagtagag catttgatgt cgactccagt tcgcatcccg cgttttaaaa tttccttcac
     2281 ttccggtcaa cgtttaaaac tatcctaatc gcgtcaaagg aggagaaatt tatgtttgtt
     2341 gaaaattacc gtcaggacaa cgtggactcc ttgatgggcg gaaataacgc cagcccgatt
     2401 ccttatccaa agatctcaga cgaggattgg cgggtttgga acttgtttct tcctgtgcgc
     2461 gcggtgaacc ttgaccggat cgccgcctcc aatgcgcgtc aagggatgat gctctcgctt
     2521 tacgacatac cgtacgccgt gacggacgag attcgtaaag ccaatccata cttcgaaaga
     2581 atcgaagtct ggcgcaagca cgagatcgaa aaggatccga ttgccgttgg gatcgccggc
     2641 ggacagcgtt acatgatcgc gcgctggggg gtggacaaac tgatcccgtt cgagagcatc
     2701 aaaaagtcgg ttcccctgat cctcgcatgg caatttatca gcagcccgct tgccgccatg
     2761 tcgggaatcg ccgcgttggg gttggttgcc tggagttttc tcgtttgacg cgtgtgcggc
     2821 gctggtgcgc tgatcagttg taagcccagg gcttgggcaa attcctttcc tgatcaggag
     2881 cgcgagattc tcctcacgct cctgattact agattcggct aataacggtc cgccgaggca
     2941 cacttgcggc gtggcgagag tagcattcgc tcactcccgc ccaccggtgg catttctata
     3001 cgcatcccta aggttcgtat tccttcgacc ttctcgctat aagagagaac aaaacggatc
     3061 ttcatcgagc atcggacacc ccattctaca caccgcaccg gtcactttcg catgaacgaa
     3121 ttcaagtatt tgcaaacccg catgccgggg attgttcgag ccttggcgat aggtatcccg
     3181 gcggggtttt tattcgagtt ccttcgcacg ccgatcccat ggatgatcgg accgatgatc
     3241 ggtgtggcct ctctgaatct gatgggcgtg caaatgcact caccccctta cgcccgccaa
     3301 atgggtcaag tgatactggg ttcggcggtt tcgctctatt ttactccgcc ggtggtcgcg
     3361 gctttgggcg gcaattggcc ggcgatcgca gccacgaccg tgaccgtttt tttggtcggc
     3421 attttcggcg cgctcacgtt gagccgtgct tcgggtgtcg atgcaaaatc aactttcttc
     3481 gcttccattc ctggaggcgc catggcgatg gctgtgctcg ccgatcggta tggcgcccag
     3541 atcgctcctg tggcggtcgc ccacagtctt agagtatcgc tggtggtgat tctcattcca
     3601 ttcgctttga cttacggcgg atttccactg gaggctgccg cgtaccggcc ggccttgccg
     3661 ctgagcattc ctattctcgc gttctggctg gctgtgggtt ggctgttggg cgaagtttca
     3721 gaaaaatttc actttcataa cggctgcctt ctggtgccga tcttcctcgg tgcggcactt
     3781 acgatgggcg gtgttcagct ctcctccgtg ccgcgctgga tgacagactt cgcacaactg
     3841 atgttcggtc tggtgctcgg tgcgcgctat gagcgcgctt tttttacccg ctataggctt
     3901 ttcatcccgt tcgctttgtt gaattcgttt tttatcctga tcgcgtcgct gctggcggga
     3961 gcggcgctcg cctgggcgtt tagcctgccg gtggctacga tgatcctcgc gacatcgccg
     4021 ggcggattgg cggaaatgac aattaccgct caggccctac acctgagtgt cccgctggtg
     4081 gtcgcttttc acctattccg ggtcgtgatt gtaaacatcg ggactcaata catttacgta
     4141 tggaccgccg ggctttttaa ccgtgctcca acggccgttc ggaagacggc accttcgatc
     4201 ggcagttaat gttacgcagg gatcagctgc acagagttcg ggctgatggt aacaatgact
     4261 tcatcccctt cggaatatcc gctctcgata aaacgtcgtc g
//
LOCUS       DASULZ010000807         2664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_94614, whole genome shotgun sequence.
ACCESSION   DASULZ010000807 DASULZ010000000
VERSION     DASULZ010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94614"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VFY96_16575"
     CDS             <1..288
                     /locus_tag="VFY96_16575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608142.1"
                     /translation="SIFLVAKSCLRSDHFSRNSRDKVPGHPSQCSAPPSRGFLKAPAS
                     MATNSGACNSDTAGTATLMSLTGISAENILAGDVSHRASVKTKLSSASSYR"
     gene            complement(297..1259)
                     /locus_tag="VFY96_16580"
     CDS             complement(297..1259)
                     /locus_tag="VFY96_16580"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5608143.1"
                     /translation="MTIVVLAALVISPPTRATSQTKNPAPTKIRLATPTGSLSYLPIH
                     VALQKGFFARRGFDMEVIQMAAGLAAPALLTRAIDYTTIPSGPATAGARGAPLKVICF
                     TSVKLQHVLISRPEFTSVAELAGKRIGAGSFGTLPAYEVRVLIDKYRLGTNTVIVPLN
                     STQDRMIGTQRGTIDATVVPVPSDLKAEEMGMKRLLAMGTILQIPQAGLATTEEKIKT
                     DRQEVQEILKATIEGIEYTWTQRDGTIAVIAKWMNLNTAQAAKAYDTVRDTFSKNCVP
                     TEEQSKAYLAMLSSTAGLKPEISPTTIFDFSPALEAAKDLSAKK"
     gene            complement(1346..1678)
                     /locus_tag="VFY96_16585"
     CDS             complement(1346..1678)
                     /locus_tag="VFY96_16585"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608144.1"
                     /translation="MSDGTINLAVLRFKTTEAADRQDGLGPVFGLHHFGFWVDDIEQT
                     RERLDQEGAEYRFGRPDTATTSFFEEKYKGPDAVMIDITAHGWVGAQPPTPNEQKLSE
                     KEHRPVRL"
     gene            complement(2083..>2664)
                     /locus_tag="VFY96_16590"
     CDS             complement(2083..>2664)
                     /locus_tag="VFY96_16590"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5608145.1"
                     /translation="ELRLQYLTEDKRGTEVLKAVAERARWQRRAAAGAKTDGTSASGR
                     GIAVTRRSGGYAAAVADVDVNRSSGKVTVKKITLAHDCGLMVNPDGVKNQVEGNIVQG
                     VSRALLEEVGFNADGVTTLDWATYPILKFSEVPEIDIALINRPEVAPLGAGELATVPV
                     PAAIASAIFDAVGAKVREAPFTPNRVLAAMKNA"
BASE COUNT          546 a          742 c          739 g          637 t
ORIGIN      
        1 tcgatctttt tagtcgcgaa gtcatgcctg cgctcagatc atttcagccg taattctcgt
       61 gataaggtcc ccgggcaccc gtcacaatgt agcgcacctc caagccgtgg ttttttaaaa
      121 gctccggcat cgatggcgac aaacagcggc gcctgtaaca gcgacaccgc cggcacggcg
      181 acattgatga gtttgacggg catctcggca gaaaatatcc ttgccggcga cgtgagccac
      241 agagccagcg ttaaaacaaa attgtcgagt gcttcatcct accgatagga aatcagctat
      301 ttttttgcgc tcaggtcttt ggccgcctcc aaagccgggg agaagtcgaa gatcgtggtg
      361 ggtgaaattt ccggcttcag accggccgtc gaactgagca ttgcaagata agccttcgac
      421 tgctcttccg tagggacgca gtttttggaa aaggtgtcgc gcaccgtatc gtacgctttg
      481 gcggcttgcg cggtattgag attcatccat ttggcaatga cggcaatggt gccgtcgcgc
      541 tgtgtccagg tgtattcgat accttcgatg gttgccttga ggatctcctg gacttcttgt
      601 cggtcggttt tgattttttc ctcggtcgtc gccaagcctg cttgtgggat ttgcaggatc
      661 gtccccatcg ccagaaggcg cttcatgccc atttcctcag ctttcagatc agagggaacc
      721 ggaaccacgg tcgcatcgat cgtgcctctt tgggtgccga tcatcctatc ttgggtggaa
      781 ttcagcggca cgataaccgt attcgtcccg agccggtatt tatcgataag cacgcgaact
      841 tcgtacgctg gcaaagttcc aaaactgcca gcgccgatcc gtttgccggc cagctcagcg
      901 acgctggtaa attcgggccg actgatgagc acatgctgaa gcttcactga agtaaagcag
      961 atgactttga gcggcgcgcc gcgtgcgcca gcggtagcgg gtccgcttgg aatcgtcgtg
     1021 taatcgatcg cgcgggtgag cagcgccggc gcggccaagc ccgccgccat ctgaatcact
     1081 tccatgtcga aaccgcgtcg cgcaaaaaag cctttttgca gcgccacatg gatgggcaga
     1141 tagctcagcg atccggttgg agtggcgagt ctgatcttgg tcggagcagg attcttagtt
     1201 tgagaagtcg cccgagtcgg aggcgagatc acgagcgccg cgagaacgac gattgtcatc
     1261 gacccaattc ttctatgtga agagagccgg ttgggcatga ttcagatgag tctctgttct
     1321 ttatgcgaag tgggagtcaa agcatttata gtctgaccgg tcgatgctct ttttcactga
     1381 gcttttgctc gttcggcgtt ggcggctgtg ctccgaccca accgtgcgct gtaatatcga
     1441 tcatgacggc gtccggaccc ttgtattttt cttcaaaaaa gctggtcgtt gccgtgtcgg
     1501 gacgtccgaa gcggtactcg gcgccttcct ggtccagccg ctcgcgcgtt tgctcgatgt
     1561 catcaaccca gaagccaaag tggtgcagac cgaagacggg tccgagtcca tcctggcgat
     1621 cggcggcttc ggtggtttta aagcgcaaga cggccagatt gatggtgccg tcgctcaaat
     1681 agattcctct ggcgatcggc gagtcggtcc gtccgactta gaccatatcg aaaacgcgct
     1741 tgtaaaactc ggcagttttt tccggatccg gtgtgtgtac tgctatatgt ctgagctttg
     1801 ccataattgt cctccttgag aaaatcacta aacgttatca gcgtgcggaa atctttttga
     1861 agaagccctc cttctccagc tcgcgcactg atcatccaaa ccagttcttc gatacggcag
     1921 cgccggaccg ctagctactc acgatgatca gtgtaaaccc gatttcccga gtagcgcaaa
     1981 gcgcatatcg agggatagag caaaccatga gcatagttca cacgctttgc cgcggaataa
     2041 aattcgacgc gtgaattcgg tgagaaccga tccgagagcc ggctacgcat ttttcatcgc
     2101 cgccaaaact ctattgggcg taaatggcgc ttctctcacc ttggcgccga cggcatcgaa
     2161 gatcgcgctg gcgatcgccg cgggaaccgg cacagtagcg agctcaccgg cgccgagggg
     2221 tgcgacctcc gggcgattga ttagggcgat gtcgatttcc gggacttcgc taaatttcaa
     2281 gatcgggtag gttgcccaat cgagggtggt gactccgtcc gcattgaacc ccacttcttc
     2341 caacagggcg cgactcacgc cttggacaat attgccttcg acctgatttt ttactccatc
     2401 cggattgacc atcagcccgc agtcgtgcgc aagcgtaatc tttttgaccg tgaccttgcc
     2461 gctggatcga ttgacgtcga cgtcggcgac cgccgcagca tagccgccag agcgacgcgt
     2521 cactgcgatg cctctgccgc tggcgctggt gccatcggtt ttcgcgccgg ctgcagcgcg
     2581 cctttgccaa cgcgctctct ccgcgacagc tttcaagact tcggtgccac gtttatcttc
     2641 ggtcaaatac tgcaaacgta actc
//
LOCUS       DASULZ010000808         3260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_804072, whole genome shotgun sequence.
ACCESSION   DASULZ010000808 DASULZ010000000
VERSION     DASULZ010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804072"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1148
                     /locus_tag="VFY96_16595"
     CDS             <1..1148
                     /locus_tag="VFY96_16595"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEX5608146.1"
                     /translation="NLLKLLSISIGNLVGNQFVTHYDWKYLSAPAMLSYDVVEWGQPL
                     ERLERQTPIPTGTEVLLKLKYCGVCHSDAHIRDGYFELGGGKRLLMSERGMRPPVTLG
                     HEPLGTVIAAGPEAVDDAIGQDRLVYPWIGCGACMRCREGMDQYCMEPQMIGIQRRGG
                     YADHLIVPHARYLVDSSGIDPLWAATLSCSGLSSFAAVSKLRPIPVEEWIAVIGAGGL
                     GLSAVGMLRALGHQRIVAVDIDREKLATAEKAGAAAALNTREGDAELDLKRITHNALF
                     GAIDFVGAAETAQLAISSLRKGGRLILVGLYGGEIPLSIAGTILRAITVRGSHLGSIE
                     ELRTVIGLARAGKIKPIPIEKRGFSDLNRTLDQLKSGSVTGRVVIEI"
     gene            1229..1621
                     /locus_tag="VFY96_16600"
     CDS             1229..1621
                     /locus_tag="VFY96_16600"
                     /inference="COORDINATES: protein motif:HMM:NF018425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX5608147.1"
                     /translation="MGAIVLVPVFILSFIAGDSGVNEYRIDLLTWLIVIIVVGGIGGL
                     YATTRYTITPDTLLVRSGPFAWMIPLREITRIEPTRNPASSPAFSLDRLSIYYGLGNR
                     LMVSPTDKENFLATLRKRMQAQKEAITR"
     gene            1729..2934
                     /locus_tag="VFY96_16605"
     CDS             1729..2934
                     /locus_tag="VFY96_16605"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5608148.1"
                     /translation="MPFSKLGLSPQVLEGVRAAGYTDPTAIQLRAIPLILAGRDVIGS
                     AQTGTGKTAAFALPLITRLGTHGKLRALVLEPTRELAAQVETALRDYARFTNLRTAVV
                     FGGTGYGRQDQSLRQGVDILVATPGRLLDQIHRRMVRLDQSEILVLDEADRMLDMGFL
                     PDVRKIIERCPRNRQTLLFSATIPPEIEQLCRWALRNPETIEIGQRRSPAETVTHALY
                     PVDVGQKQELLEELLRRTEYDQVLIFCRTKNGADRVARKLHQEGHAVAVLHSSRTQRE
                     RENALAGFRNGRYEVMVATDIAARGIDVEQISHVINFDVPHHPEDYVHRIGRTGRAQS
                     VGDAFTIMTAEDLQEVAAIERFIGQKIPRVKIEGFDYAYCRLLDPNPKPAFGTRSGRR
                     SYAPGRGRW"
     gene            2956..>3260
                     /locus_tag="VFY96_16610"
     CDS             2956..>3260
                     /locus_tag="VFY96_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007746136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX5608149.1"
                     /translation="MKPILDLPAFVHAAGDAIIAAGADGRIIFWNPAATRIFGFNEAE
                     ALGQSLDLIIPERFRARHWEGYRQVMATGKSKYAADVLRVPATAKDGRALSIAFTVT"
BASE COUNT          676 a          892 c          966 g          726 t
ORIGIN      
        1 tcaatttgct gaaattgctc tcgatttcta tcggaaatct cgttggaaat caatttgtaa
       61 cacattatga ttggaaatat cttagcgctc ccgccatgct gagttacgat gttgttgaat
      121 ggggccagcc gctcgaaaga ctcgagcggc aaacgcccat acccaccggt accgaagttc
      181 tcctgaagct aaaatattgc ggcgtttgcc acagcgacgc tcatattcgc gacggctatt
      241 ttgagctggg tggaggcaaa cgtctgctga tgagcgaacg cggtatgagg ccgccggtta
      301 cgcttggaca tgagccgctc ggcactgtga tcgccgcggg gccagaagct gtcgatgatg
      361 cgatcggcca ggatcgttta gtttacccat ggatcggctg cggggcgtgc atgcgttgtc
      421 gcgaaggcat ggatcagtat tgcatggagc cgcagatgat cggcatccag cgtcgaggcg
      481 gatacgccga tcacttgatc gtgccgcatg cgcgctatct cgtcgattcg tcaggcatcg
      541 atccgctctg ggcggctact ctgtcgtgct ccggactgtc gtctttcgcc gcagtgtcca
      601 agctccgtcc gattcccgtc gaagaatgga ttgcggttat cggtgctggc ggtctgggcc
      661 tctcagccgt tgggatgctg cgcgcactcg gccatcaacg aatcgtcgcg gtcgatatcg
      721 acagagagaa gctcgccacg gcggagaagg cgggtgcggc agcggctctg aatacgcggg
      781 aaggcgatgc tgagctggat ctgaaaagaa ttacccacaa tgcgcttttc ggcgcaatcg
      841 atttcgtcgg tgccgcggaa acagcgcagc tcgcgatcag ctcgctgcgg aaggggggcc
      901 gattgattct cgtcggtctc tacggcgggg aaattccgct ctcgatcgca ggcacgatcc
      961 tgcgtgcgat cacggtacga ggctctcatc tcggcagtat cgaagaactg agaacggtga
     1021 tcgggcttgc gcgcgccggg aagatcaagc cgatccctat cgagaagcgt ggtttttctg
     1081 atctcaaccg caccttggat caattgaaaa gcgggtcagt caccggcaga gtagttatag
     1141 aaatatagct caggcaggtg ttccgttgag ggagcccacg ccgcaatcga ccaatatagt
     1201 ttttcgatcc aaaatagatc tttggctggt gggtgcgata gttctcgtgc cggtattcat
     1261 actttcattt attgccggcg acagtggagt caacgagtac cgcattgacc tgctcacgtg
     1321 gctaatcgtc attatcgtcg tcggcggcat cggcgggctt tacgcaacta ctcgctacac
     1381 gatcacgccg gatacgctct tggtcaggtc aggtcctttt gcttggatga ttccgctgcg
     1441 cgaaattaca cgcattgagc caacgcgcaa cccggcctcc agtccggcgt tctcgctgga
     1501 tcggttgagc atctactacg gtctaggcaa tcgcttgatg gtctctccga cagacaaaga
     1561 aaacttcctg gcgactctga gaaagcgaat gcaagcgcag aaggaggcga tcactcggta
     1621 gccgcagcgc atctccgaga aatcctgaaa cggcttgccc ggtccacagt attgacacag
     1681 ataacgttca cgtttgtgaa ggcgcgcggg ttgtgtttat aagagtctgt gccattttca
     1741 aagcttggtc tttcgcctca ggtgcttgaa ggcgtgcgcg ccgccggtta tacggatccg
     1801 acggcgattc aactccgcgc gattcccctt attttggccg gtcgggacgt tattggttcg
     1861 gcgcaaaccg gaactggaaa aactgccgcg ttcgccctgc cgttgattac ccgccttggc
     1921 acgcacggca agctgcgcgc gctggtgctc gagccgaccc gtgagctcgc ggcgcaggtt
     1981 gaaacggcgc ttcgcgatta tgcacgcttt accaacttgc gcaccgccgt cgtcttcggg
     2041 ggcaccggtt acggcaggca ggatcagagt ttgcggcaag gcgtggacat cctggttgcg
     2101 acgccgggtc gcctcctcga tcaaatccac cggcgcatgg tacggctcga tcagagcgaa
     2161 attttggttc tcgatgaggc cgaccgcatg ctcgacatgg ggtttttgcc ggatgtgcga
     2221 aaaattatcg aacggtgccc gcgcaaccgc caaaccctgc tgttttctgc gacgataccg
     2281 ccggagatcg aacagctttg cagatgggca ttgcgcaatc cagagactat tgaaattggt
     2341 cagcgacgct cgcccgccga aactgtgacc catgctctct atccagtcga tgtgggtcag
     2401 aagcaggaat tgctggagga attgctccgg cgcaccgagt acgatcaggt gttgattttc
     2461 tgccggacga aaaacggtgc tgaccgggtc gcgcgcaagt tgcatcagga aggccacgct
     2521 gtcgctgtcc tgcattcgag ccgaacacaa cgcgaacggg aaaacgccct tgctggtttc
     2581 cgcaatggca gatatgaagt catggtcgcg accgatatcg ctgcgcgcgg tatcgatgtc
     2641 gagcagatca gccatgtcat caactttgac gtgccgcatc atccggaaga ctatgtccat
     2701 cggattggcc gcaccggacg cgcacagagt gtcggcgatg ccttcacgat catgacggcg
     2761 gaggatttac aggaggtggc ggccattgag cgcttcatcg gtcagaaaat cccccgtgta
     2821 aagatcgaag gattcgatta cgcgtattgt cgtctgcttg atccgaatcc aaaaccggct
     2881 ttcggcaccc gctcgggcag acgctcctac gctccgggac gcggccgctg gtaattcaag
     2941 tcgaactggc tgacgatgaa accaatactc gacttacccg cttttgtgca cgcggccggc
     3001 gatgcaatca tcgccgcggg agccgatggc aggattatct tctggaatcc tgcggcgacg
     3061 cgaatctttg gatttaacga ggcggaagct ctgggtcaat ccctcgattt gatcattccg
     3121 gagcgttttc gcgcacggca ttgggaaggc tatcgccagg tcatggcaac cgggaaaagc
     3181 aaatatgcgg ctgatgtgct gcgagtgccg gcgaccgcta aagacggccg cgcgctgtcc
     3241 atcgcattta cggtaacttt
//
LOCUS       DASULZ010000809         3529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_296713, whole genome shotgun sequence.
ACCESSION   DASULZ010000809 DASULZ010000000
VERSION     DASULZ010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296713"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2700)
                     /locus_tag="VFY96_16615"
     CDS             complement(<1..2700)
                     /locus_tag="VFY96_16615"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5608150.1"
                     /translation="MAEHELDAVLSVAAATSGCSLALLSFMDEEREWFKAKIGFDLSE
                     LSLDNSFCAHMVSGSEPLIVSDTAADPRFCKNSLVLNPPGIRFYAGFPITADGHAVGA
                     LAVLDRQSRQLSAQQISQMEVLSRLISDRLQLRRTEDLLAASRAVNREQEARLRENQE
                     VETRLIEWSPDCIAVLDLDGRVGTMNLGGMQALEICDTEELSSSSWTDFWKGRDVSLA
                     LEAIESARRGGVGRFTGFRATQSGIPKWWDVMVSPIRDPTGSPTQLVAVMRDISERRR
                     AVQMLRVVSEGTAAVTGGDFFRSLVQHLAGALDARYSFVAECTDPTKTKVRTLAYWQG
                     GDFGADVTFLLRGTPCEKVIGGEVCCYPERLQSLFPEEAALVRLNAQSYLGVPIHAAS
                     GDILGHLVVLDDKPMGDMEYAVPILRIFAARAGSELERSRTEASLKNSEARRRMLLEI
                     NNAIVTKLTRDELWSAIAEAMSRVVAFDRIALALYDPQENSLRIVSYAGSYTRDDYTP
                     LGRRLKLDDSPAGLAFVARKPVLRRDLELERLTSSEERAYGHGFRSICALPLVVRRLS
                     IGAITLGSLNRSQYSESDADFLMQVANQIAIAVDNLRAHEETEALKARFQAEAVYLQE
                     EIKTEHNFEEIIGQSTAIRALLKKLEQVAPTDATVLIHGETGTGKELLARAVHDRSRR
                     KTRPLVKVNCGSIPSGLVESELFGHERGAFTGATQQRIGRFELAHGGTIFLDEVTELP
                     LETQVKLLRVLQEGEFERVGSSRTIKVDVRVIAATNRNLQEVVRSGIFRSDLFYRLNV
                     FPLDVPALNERREDIPLLVNFFLGKFSKSVGKEIRGLSQKSMERLVQYGWPGNIRELQ
                     NVVERAVVLAKGAIVQIDDPMLGAEASPEEANIETLENVERNHI"
     gene            2938..3213
                     /locus_tag="VFY96_16620"
     CDS             2938..3213
                     /locus_tag="VFY96_16620"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX5608151.1"
                     /translation="MLKITKMVENGSVVKLRLDGTITSESFAEIDRTRAQYQSEANRR
                     VVLDMAGVTFMQTDPAQKLAQLKRDSLRIINCSPFIAALLETAENTD"
     gene            3259..>3529
                     /locus_tag="VFY96_16625"
     CDS             3259..>3529
                     /locus_tag="VFY96_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608152.1"
                     /translation="MMLSGSETLVVRSLIPANPLPLRSRKSTRRSKGICRPAQSFAAA
                     ADQTELIERLKSGDQRALETIFDLYSVKLYNVALRILGEPADTEEV"
BASE COUNT          801 a         1058 c          908 g          762 t
ORIGIN      
        1 gatgtgattc cgttccacgt tttccagcgt ctcgatgttt gcttcttcgg ggctcgcttc
       61 agcgcccaac atcgggtcat cgatttgcac gatcgcgcct ttcgccagca caaccgctct
      121 ttcgacaaca ttttgtagct cgcgaatatt tcccggccaa ccgtactgaa ccagacgttc
      181 catggatttt tgtgacagcc ctcgaatttc tttgcccacg ctcttgctga atttgccgag
      241 gaaaaaattc accaaaagcg gaatatcctc gcgccgctcg ttcagcgccg ggacgtcgag
      301 cggaaagacg tttagccgat aaaacagatc cgagcgaaag atcccgcttc gcactacttc
      361 ctgcaaattg cggttggtcg cggctatcac acgcacatcc accttgatgg tccgactgct
      421 ccccacccgc tcgaactcgc cttcttgaag gacgcgcaag agcttcacct gcgtttcgag
      481 cggcaactcg gtgacctcgt cgaggaatat tgttccaccg tgcgccagtt cgaatcgccc
      541 gatacgctgc tgtgtcgctc cggtgaaggc gccgcgctca tgcccaaaca attcactttc
      601 caccagaccc gacgggatcg atccgcaatt caccttcact agcggccgcg ttttgcggcg
      661 gcttcgatcg tgcacagccc gcgccagcag ctctttgccc gtgccggttt cgccgtggat
      721 caaaaccgtt gcgtcggtgg gcgccacttg ctcgagcttt tttaaaagcg cgcgaatcgc
      781 cgtgctctgc ccgataattt cttcgaagtt gtgctcggtt ttgatctctt cttggagata
      841 aaccgcctcg gcttggaagc gcgctttcaa cgcttcggtt tcttcgtgcg ctctcaagtt
      901 gtccactgcg atcgcgatct ggttggctac ttgcatcagg aaatccgcgt cgctttctga
      961 atactgcgaa cgattcagac tgcctagcgt aattgcgcca atgctcaagc ggcgcacgac
     1021 cagcggcaaa gcgcagatcg agcgaaagcc atggccgtaa gcccgttcct cggaactggt
     1081 caagcgttcc agctcgagat cgcgccgcaa gaccggcttg cgcgcaacga aagcaagacc
     1141 ggcgggactg tcgtccagtt tgagccggcg gccaagcggc gtgtaatcgt cgcgggtata
     1201 cgaccccgca tagctcacga tgcgcaagga attttcctgc ggatcgtata aagcgagggc
     1261 gatgcgatcg aaagcgacga cccgactcat cgcctcggcg atcgcactcc agagttcatc
     1321 ccgggtaagt ttggtcacga tggcattgtt gatttccagc aacatgcgcc tgcgcgcctc
     1381 gctgtttttg agcgaggcct cggtgcgact gcgttccaat tccgagccgg cgcgagcggc
     1441 aaagatacgc aggatcggca ctgcgtattc catatccccc atgggcttgt catcgagcac
     1501 caccaaatgt ccaaggatat cgcccgaagc tgcatgaatg ggaactccaa gatagctttg
     1561 cgcgttcagg cgaaccagag cagcctcttc gggaaaaaga gattgcaagc gctccgggta
     1621 gcagcaaacc tcgccgccaa tcactttctc gcaaggcgtc cccctgagta agaaagtaac
     1681 atctgcgccg aagtcaccac cttgccagta tgccaaggtt cgaactttag tttttgtcgg
     1741 gtcggtgcat tccgcgacaa atgagtagcg cgcgtcaagc gcgccggcca gatgctgcac
     1801 caacgaacgg aaaaaatccc cgccagtgac agccgcagtg ccctccgaaa ccacgcgcaa
     1861 catctgcacc gcacggcgac gttcgctaat atcgcgcatg accgccacca gttgcgtcgg
     1921 acttccagtc gggtctcgga tcgggctgac catcacatcc caccatttgg gaataccgga
     1981 ctgggtggcg cgaaatccag tgaaacgtcc cacgcctcca cgccgtgccg attcgatggc
     2041 ctcgagcgcc agcgagacat ctctcccttt ccaaaaatct gtccaggaag aactgctcag
     2101 ctcttcagtg tcgcaaattt ccaatgcttg catcccaccc aaattcatcg tcccgacgcg
     2161 gccgtcaaga tcgagaaccg caatacaatc cggactccac tcgattagcc tggtttcgac
     2221 ttcctgattt tcgcgcagcc ttgcctcctg ctcgcgattg acggcgcgcg aggcggcaag
     2281 taagtcttcc gtccggcgta actgcaggcg atcggaaatc aacctgctta acacttccat
     2341 ttggcttatc tgctgtgctg agagttgccg cgattgacga tccagcaccg ctaaggcgcc
     2401 gaccgcatgc ccgtccgccg tgatcggaaa gcctgcatag aagcgaatcc ccggtggatt
     2461 gagaacgagc gaatttttgc aaaaccgcgg atccgcagcc gtatcggaaa cgatcaatgg
     2521 ctcgctgcct gaaaccatgt gagcgcaaaa tgagttatca agcgatagct cgctcaaatc
     2581 aaaaccaatc ttagctttga accactcccg ctcttcatcc atgaagctta gtaaggcgag
     2641 cgaacatccc gaggttgccg cagcaaccga aagcaccgcg tcgagttcat gctcggccaa
     2701 agtctccccc tttaattgct tgagagctga caaactcgcg gtctgatctt ttaaatcgcc
     2761 gagattcacg tgaccagaat tattccgttt tgcggcattt cgcaagcatc acgatatttc
     2821 gcgacgaaat accgcatcgc aatctccttc tggggagaaa cgggcgcaaa tacaatacct
     2881 taggagtatg gcacatccgc tgctctacac gtatgcgcga ggacaaacga ggcactgatg
     2941 ctcaaaataa ccaaaatggt cgagaatggc agcgtagtga agctccgctt ggacggcacc
     3001 ataacttcgg agtcttttgc ggagattgat cgcacgcgcg ctcaatacca atcagaagcc
     3061 aatcgtagag tagttttgga tatggctggt gtaactttca tgcagaccga cccggcgcag
     3121 aaattggcgc agctcaaacg cgacagtctg cgaatcatca attgttcccc tttcatcgcc
     3181 gctctgctgg agacggcgga aaacaccgac tgaatttttc atggctaaag atttgccaac
     3241 gcgcaagagg agatgagtat gatgctgtcc ggatcagaaa cattggtcgt gagaagtcta
     3301 ataccggcaa acccactgcc gcttcgatcg cgaaagtcga cgcgccgctc caagggtata
     3361 tgccgccccg cgcagagctt cgctgccgca gccgaccaga cggagttgat cgagcgcctg
     3421 aaatccggtg atcagcgagc gctggaaact atcttcgatc tctattccgt caaactctac
     3481 aacgtggcgt tgcggatctt aggcgaaccg gcagacactg aggaagtga
//
LOCUS       DASULZ010000810         6833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_804263, whole genome shotgun sequence.
ACCESSION   DASULZ010000810 DASULZ010000000
VERSION     DASULZ010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6833
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804263"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="VFY96_16630"
     CDS             complement(<1..649)
                     /locus_tag="VFY96_16630"
                     /inference="COORDINATES: protein motif:HMM:NF014136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5608153.1"
                     /translation="MANAAVSIEEEVKQRIAYKSPYEQWKESEGLPTYRGLIVKNLYE
                     LELAPWRSSGVGSAAFLNLDGTGGFNDAYVAEIPAGKSLKAHRHLFEETIYVLKGQGA
                     SSIWIEPEKKETFEWQEGSYFSIPMNAWHEHHNASGMEPARYIAMTSAPRVIDTFNNY
                     EFVFDNPFVFRDRYNGEQGYFKQTAPAQQWSTNFIADVRVCQMFSSGFDRGGGAKS"
     gene            complement(729..1763)
                     /locus_tag="VFY96_16635"
     CDS             complement(729..1763)
                     /locus_tag="VFY96_16635"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5608154.1"
                     /translation="MEVKSVKEVHTELLMHSVRFAKDEQGRRKGQDVVFKWPEIEKKL
                     GEHKHIYVVDARLGSNNHTHRLWYDIIPPHTEEGQDWRTLGHRHTVEAIIYFLKGHGH
                     SIIDGERYDWGPGDLLSVPMFSWHRHINDSDEPVLRIASTTGPLSLALGQAVYEDERF
                     PEFWIYAQEGEDARKTLIPGGGGQLDNGQTPHNRTAEMYAEQVAFAMHEEQLRRKSKV
                     LVRQKDLVFEPTAMGNLAYVVDPRIGCHSKALGVVMAEIPPGKRSGAHRHLYDEIDLI
                     VGGKGKVIVEDKEFDFDTLDVLSIPVFQWHQYFNTGSEPLRILGINTRLAMDNLGLSL
                     THQGEHADYV"
     gene            complement(2101..3000)
                     /gene="nhaR"
                     /locus_tag="VFY96_16640"
     CDS             complement(2101..3000)
                     /gene="nhaR"
                     /locus_tag="VFY96_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013936067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HEX5608155.1"
                     /translation="MEWLNYHHLLYFWTVARLSSVSRASEELHLTQATVSAQLKSLEA
                     SLGESLFRKTGRHLILTETGKVVFRYADEIFSLGREMMGTLKGRPQGRSARLTVGVAD
                     VMPKLVAFQLIEPALKLADSHRIICREGTNKELLSALAVHDIDVVLTDSQIDPAINVK
                     AFCHLLGECGMTLFGTAKLATLYRHQFPQCLDGAPFLLPTSNTAVRRSLDQWFETETI
                     RPAIVAECEDSALLQVFGQRGLGLFFAPSVISAEVRRQYGVKIIGKVSAVQEQFFAVS
                     LDRKLKHPAVVAISEAARARLFD"
     gene            3119..3547
                     /locus_tag="VFY96_16645"
     CDS             3119..3547
                     /locus_tag="VFY96_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608156.1"
                     /translation="MRATTPMMFNPDGLKLFEPDTLAPEQFYATIRKTQFADPERRLM
                     AAVLEDAVSCLSSNPRPCAGRKRRYFEEAHSWINATEEQDWIFSFTNVCETLGFDPSF
                     LRRGLNRWNSTTAEKGLPPLRLKKYRSGARRRKLRFRSVI"
     gene            3793..4881
                     /locus_tag="VFY96_16650"
     CDS             3793..4881
                     /locus_tag="VFY96_16650"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEX5608157.1"
                     /translation="MSKQSSLTDFHRENGAVFAEYERWLLPRHFGDPAAEYRVLRGAA
                     GLIDLSQRALLQFTGPDRLSFLQGMLSNDLRSLNPFDGQYSAILTQQGKVIADVRVLC
                     AMNSFYLDFWENLKDNVIGHVQRYLVADDVEISDRSADYTFLAIRGPSAEMVLQKLLS
                     DTPLPTRPKQHAMIDIEGAAICIIREERGGNPGFDLVVPRANASNIAHVLTQTGRQFS
                     AAWAGEEAQNILRVENGIPRYGPDFNENNLLLEVGIDDAFSFTKGCYLGQEIVERIRS
                     RGHVNKKLCGLVLDGSSPAAAGDSIHTDGKVVGTITSSVLSPRLNRPIALGYLGKEYW
                     NPGTVVSVEHSGSMIGGTVSRVPFIASS"
     gene            4945..5253
                     /locus_tag="VFY96_16655"
     CDS             4945..5253
                     /locus_tag="VFY96_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5608158.1"
                     /translation="MANFVKVCKTGDVKPGSGRSLDINGKAIGIFNVDGKFYAINDVC
                     GHRGGPLGEGEMDGTTVICPWHGWRYNVTTGENELLPNLPTQKFEVKVEEDDVLVDLS
                     "
     gene            complement(5283..5831)
                     /locus_tag="VFY96_16660"
     CDS             complement(5283..5831)
                     /locus_tag="VFY96_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5608159.1"
                     /translation="MASEHELPHAHAHGHVEFRFCPRCGGGLDKKIVKASEPRRLVCQ
                     SCAFIFYQDPKVVAGTIFMLDGGIVLLKRGVEPALGKWVFPGGYVDRGESVRDAAVRE
                     TKEESQLEVKLGSLLNVYTYPRSPNVIIVYTAEVISGELAAADESLEARIFAPNELPW
                     GELAFESTKDALKDYVNIYVKS"
     gene            5891..6574
                     /locus_tag="VFY96_16665"
     CDS             5891..6574
                     /locus_tag="VFY96_16665"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX5608160.1"
                     /translation="MLRAVIFDFNGIIVDDEPIHFKLFQRVFAEEGIDLSQAAYYQRY
                     LGFDDRGAFLAGFRDHGRAVGAAKLQELIARKADYYQEAIRNHVAIFPGVKALVEDLS
                     RTLPLAVASGALRQEIETILQTAGLFNRFRAIVSAEDVQQGKPEPEIFIKALAGLNAA
                     SGDRGGIAPTDCVVIEDSKEGVRGARRAGMKCLAVTNSHPAELLKEADAIVASLEEVA
                     LPFLHRLCG"
BASE COUNT         1517 a         1891 c         1815 g         1610 t
ORIGIN      
        1 tactcttggc gccgccgccg cgatcgaagc cgctcgagaa catctggcac acacgaacat
       61 cggcgataaa gttggtcgac cactgttgcg ccggcgccgt ttgcttgaag tagccttgct
      121 cgccgttgta acgatcccga aaaacaaacg ggttatcgaa aacaaattcg tagttgttga
      181 aggtgtcgat gacccgtggc gcgctggtca tggcgatgta gcgcgccggc tccatgccgg
      241 aagcgttatg atgttcgtgc caggcgttca tcggaatcga aaaatagctt ccttcttgcc
      301 actcaaaagt ctccttcttc tccggttcga tccagatcga gctcgcgccc tggcctttga
      361 ggacgtagat cgtttcttcg aaaagatggc ggtgggcttt cagcgatttt cccgcgggta
      421 tttccgcaac ataggcgtcg ttgaagccgc cggtgccgtc gagattgaga aacgccgcag
      481 agcccacgcc gctcgaacgc cacggcgcga gttcgagctc gtaaagattt ttgacgatta
      541 aaccgcggta ggtcggcaag ccctccgatt ctttccactg ctcatacgga gatttataag
      601 cgatccgttg tttgacttcc tcttcaatgc tcactgcagc gttggccatg aattgatttt
      661 cctttttcag gtaccagtcg ccattctgcg ctgggggatt tctcagttcc cgacacgcta
      721 atcaacgttc aaacgtaatc cgcatgttcg ccctgatgcg ttaacgagag tcccaaattg
      781 tccattgcca atcgcgtatt gatgccgaga atgcgcaaag gttcgcttcc ggtgttgaaa
      841 tattggtgcc attgaaaaac cgggatcgag agcacgtcca atgtatcgaa atcgaattct
      901 ttgtcttcaa cgatcacttt gcccttccca ccgacgatca ggtcgatctc atcgtacaga
      961 tgccgatgcg cgcccgagcg tttgcccgga ggaatttccg ccatcacgac gccgagcgct
     1021 ttgctatgac agccgatgcg gggatcgacg acataggcga gattgcccat cgccgtcggt
     1081 tcgaaaacca gatccttttg cctgacgagc actttgctct tccgcctcag ctgttcttca
     1141 tgcatcgcga acgccacctg ttccgcatac atttcagccg tgcggttatg cggcgtttga
     1201 ccgttgtcga gctgcccacc tcccccggga atcagtgtct tgcgcgcgtc ctcgccctcc
     1261 tgggcataga tccagaattc gggaaaacgc tcatcttcat aaaccgcttg accgagagcc
     1321 agcgacaacg ggccggtggt ggatgcgatg cgcaacacgg gctcgtcgct gtcgttgatg
     1381 tgtcgatgcc aggagaacat cggcacgctc agcaagtcgc cggggcccca gtcgtagcgc
     1441 tccccgtcaa tgatgctgtg gccgtggccc tttaaaaaat aaatgatcgc ctccaccgta
     1501 tggcgatggc cgagagtacg ccagtcctga ccctcttcgg tgtgcggggg gatgatatcg
     1561 taccaaagtc gatgggtgtg attatttgac ccgaggcgcg cgtccactac gtagatatgt
     1621 ttatgctcgc ccagtttctt ctcgatctcc ggccatttga acacgacgtc ctgccctttg
     1681 cgccggccct gttcgtcttt ggcaaagcgt acgctatgca tcagcaattc tgtatgtact
     1741 tctttgaccg atttaacttc catgaatgcc tccccggcga aaataattcg ctagagctta
     1801 tgtaatggcg tcgactctat cgcaaggctg gcagctttcc aacaattcag cttacggtca
     1861 caacgagaaa atccgcgctc gacatcgaag tcgtcacttc cgaatctcat ttgggagttc
     1921 aattcgaatt tcgtctggat tcccgctaaa gcatacggga actgacggca cgagagttcc
     1981 cctcttaaca tctcttgcat ttccgaaagc tgtcgtaggg tgcgcttgcg caccactcca
     2041 gaagtgtcgc gatttctgac ttgaacttca gttttagaac cgcgcggcag acaagcttgt
     2101 tcagtcaaaa agccgcgcgc gtgcggcttc cgagatcgca accaccgcag gatgtttgag
     2161 ctttcgatcc agtgaaaccg cgaagaactg ttcttgaact gcgctcacct tgccaataat
     2221 tttgacgcca tactggcgcc gcacctcggc actaatcacc gacggagcga aaaacaatcc
     2281 aagcccgcgc tgcccaaaaa cttgcagtag agcactatct tcgcattcgg caacgatcgc
     2341 cgggcgaatt gtttctgtct caaaccattg gtcgagtgac cgccggaccg ccgtattcga
     2401 ggtcggcagc aaaaagggcg cgccatcgag acattgagga aattggtggc gatagagcgt
     2461 tgccaatttc gccgttccaa aaagggtcat gccgcactcg ccgagcaaat ggcagaatgc
     2521 cttcacgttt attgccggat cgatttggct atcggtcaag acaacgtcaa tatcgtgcac
     2581 cgccaatgcc gaaagtagtt ctttattcgt tccctcgcga cagatgatcc gatgggaatc
     2641 ggcgagtttc agggccggct caatcaactg aaaagccacc agcttgggca tcacgtcggc
     2701 gacgccaacc gtaagacgcg cagaacgccc ctgcgggcgc cccttgagcg tccccatcat
     2761 ttcccggcca agggaaaaga tctcatcggc gtagcgaaac acgaccttcc ctgtctctgt
     2821 aagaatcagg tggcgccccg ttttgcgaaa cagactttcg cccagcgacg cctcgaggga
     2881 tttgagctgc gcgctcaccg tggcctgagt caaatgcaac tcctcactgg cgcgtgagac
     2941 actgcttagg cgagcgacgg tccaaaagta caaaagatgg tgataattca gccattccat
     3001 gccctgaata ttagtttgat tttttctatg aaacaataag aaattatgaa tttgcctatg
     3061 tattggtttc ttcctaagct gctcacagat tctcgagctt cgctcacagg ggtcttatat
     3121 gagagctacg acaccgatga tgttcaatcc agatggacta aagcttttcg agccggatac
     3181 gcttgcgccg gagcagtttt acgccaccat taggaagacc caattcgcgg acccggaacg
     3241 gaggctaatg gctgccgttc tcgaggacgc ggtctcctgc ctgtcgtcga acccgcgtcc
     3301 gtgtgccggg cgaaaacgca ggtactttga ggaggcacac tcttggatca acgccacgga
     3361 agaacaagac tggatatttt cattcaccaa tgtttgtgaa accttgggct tcgatccaag
     3421 ctttctgcga cgtggactga accgctggaa cagcacgacg gccgaaaagg gattgccgcc
     3481 gctgcggtta aagaaatatc gctccggcgc tcgtcggcga aaattgcgct tccgcagcgt
     3541 tatttgacac gctccggttt ggttggagtc atcacgcgca gcgttattta gctcgccacc
     3601 ctgccattca cctccggcct tctgtcgaaa aagcactaac gatacgcggc cgacttttgc
     3661 tactgagatt tactggtatc tgtcgatttc gaaggaatta agaggttcct tcaactgaat
     3721 tgacgaactt ttaagatgaa ccagcgccat tgcattgtca tcccgtaggt caggcggtat
     3781 attttcgatc atatgagcaa gcaatccagc ctgacggatt tccatcgtga aaacggtgcg
     3841 gttttcgccg agtacgaacg ctggctttta ccgcggcatt ttggcgatcc cgcggctgag
     3901 tatcgggtcc tacgtggtgc ggccgggctg atcgatcttt cccagcgcgc gttgctgcag
     3961 ttcaccggtc ccgatcgcct ttcttttttg cagggaatgc tctcgaacga tctacgcagc
     4021 ttgaaccctt ttgacggcca atattcggcg attctgaccc aacagggcaa agtgatcgcc
     4081 gacgtcaggg tgctttgcgc gatgaattcg ttctatctcg atttttggga aaatctgaaa
     4141 gacaatgtga tcgggcatgt tcaacgttat ctcgtcgccg acgatgtgga aatttccgat
     4201 cgctccgccg attatacgtt tcttgccatt cgcggcccga gcgctgaaat ggtcctgcaa
     4261 aaactgttaa gcgacacgcc gctgccgact cgccctaaac agcatgcgat gatcgacatc
     4321 gagggcgcgg cgatctgcat catcagggaa gagcgcggcg gcaatccagg gttcgatctc
     4381 gtggtgcccc gcgccaacgc cagtaacatt gcgcatgttt tgacccaaac cggccgacaa
     4441 ttttctgccg cctgggccgg tgaagaggcg caaaatatcc tgcgcgtcga gaacggtatt
     4501 ccccgctatg gtcccgactt caacgaaaac aacttattgc tcgaggtcgg catcgacgac
     4561 gcgttcagct ttaccaaggg gtgctacttg ggccaggaaa tcgtcgagcg cattcgttcc
     4621 cggggtcatg tgaacaagaa actctgcgga ctcgtgcttg acggttcatc tcctgcggcc
     4681 gccggcgatt cgatccacac cgacggcaaa gtcgtcggta cgatcaccag ctccgtacta
     4741 tcgccccgac tgaatcgccc catcgctttg gggtatctgg gcaaggaata ctggaatcca
     4801 ggaacagtcg tatccgttga acacagcgga tcgatgatcg gcggcaccgt cagtagggtt
     4861 ccgtttattg cctcctcttg aatccactcg tcaacctcgt atagttacgg aaattttggg
     4921 cgggcatggg aagggatcta aagcatggcg aatttcgtaa aggtttgtaa aaccggcgat
     4981 gttaaacctg gctccggccg gagcctcgat atcaacggaa aagcgatcgg tatttttaat
     5041 gtcgatggca aattttacgc gatcaacgat gtgtgcgggc atcggggcgg tcccctgggc
     5101 gaaggtgaga tggatgggac tacggtaatt tgcccgtggc acggctggcg ttacaacgtc
     5161 actacgggag aaaacgagct gcttcccaat ctgcccacgc aaaaattcga ggttaaggtg
     5221 gaagaggacg acgtgttggt ggatttgtcc tgagcccagc aattagcggt tgtattcaga
     5281 tcctaagatt tcacataaat atttacgtaa tcctttagcg cgtccttggt gctttcgaac
     5341 gcgagttcgc cccacgggag ctcgtttgga gcaaagatgc gtgcctccag ggattcatcc
     5401 gccgcggcga gttcgccgct gatcacctcc gcagtataga caatgatgac attgggcgat
     5461 cgcgggtatg tataaacatt gagcagcgac ccgagcttta cttccaattg cgactcctct
     5521 ttggtctccc gcaccgcggc gtcacgaaca ctctcgccac gatccacata gccgccggga
     5581 aaaacccact tgccaagggc gggctcgacg cctcgtttca gtaagacaat gccgccatcg
     5641 agcataaaaa tcgtccccgc cacaaccttc gggtcctggt aaaaaataaa cgcacaggat
     5701 tggcagacca gccggcgagg ctcgctggcc ttgacgattt ttttatcgag gccgccgccg
     5761 cagcgcgggc agaaacgaaa ttccacgtga ccgtgggcgt gcgcgtgggg caattcatgt
     5821 tctgatgcca tgcttgttgt ttagctcata ttaagttgaa acaaaaggtg aattctgcaa
     5881 tcgtgatgcg atgctccggg ctgtgatttt cgatttcaac ggcattatcg tcgatgacga
     5941 gccgattcat ttcaaattgt ttcagcgcgt ttttgccgaa gaaggcatcg atctcagtca
     6001 agcagcttat taccagcgct atctcggttt cgacgatcgc ggcgcatttc tcgccggatt
     6061 ccgtgatcac ggtagagccg tcggcgccgc aaagctgcag gagctcatcg ctcgcaaagc
     6121 cgattactac caggaggcga tacgcaatca tgtcgcgatt tttcccggcg tgaaagctct
     6181 ggtcgaagat ctgtcgcgca ccctcccgct tgccgtcgcc tccggcgcgc tgcgccagga
     6241 gatcgagact attttgcaga ccgccgggct attcaaccgt ttccgcgcca tcgttagcgc
     6301 cgaggatgtt cagcaaggga agcccgagcc ggaaattttt atcaaggctc tggctggatt
     6361 gaatgcggca agcggcgaca gaggcggaat tgcaccaacg gactgtgttg tgatcgagga
     6421 ttccaaagaa ggtgtacgcg gcgcccgccg cgccggcatg aagtgcctgg cagtgacgaa
     6481 ctcccatccg gcagagctct tgaaagaagc cgatgcgatc gttgccagcc tcgaagaagt
     6541 cgcactgccg ttcctgcacc ggctatgtgg gtgacgatca gattgaaact gttcaagctg
     6601 ttcaattcgt tcaagtcgtt taagcctctc cttacatcac ccccagtcga gactgatcta
     6661 tgcgacgatc ccattgacgt cgttctgagc cggaaggcga agaatctcgt atttcctacc
     6721 gcttgaacgg tttgaacggc ttgaacaagt agagttagcg atacggagtt cgctgcggtc
     6781 tgacggtgat ttccaccggg caacatgtcg cggcggacgt gagcacgtaa taa
//
LOCUS       DASULZ010000811         2406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_907006, whole genome shotgun sequence.
ACCESSION   DASULZ010000811 DASULZ010000000
VERSION     DASULZ010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907006"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VFY96_16670"
     CDS             <1..547
                     /locus_tag="VFY96_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX5608161.1"
                     /translation="GFGDPGARVLIVGLAPAAHGGNRTGRIFTGDRSGDWLYGALHTA
                     GFANQPNSSHRNDGLKLSDCYITAAVRCAPPENKPTLAEFLRCRPYLIEELKLLTGIK
                     VVIVLGKIAFDAFLAAYSGCGGVIPKPRPKFGHGAAVDLADGPCLICSYHPSQQNTFT
                     GKLTRAMFDNVFAQARAALDK"
     gene            563..1444
                     /locus_tag="VFY96_16675"
     CDS             563..1444
                     /locus_tag="VFY96_16675"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5608162.1"
                     /translation="MEMGVVYALCAGVFWGTSPVLVKRGLAGADVSAATLYQQATILL
                     TLCFSALLEGDLFAGKIPGKAVLIFAGTGVVGAYIGRTLFVKSVDEIGASRAQSLNNS
                     SPLITVLLAAVALSERLSLAVLIGVIFIISGVFFVTRDDATGLGKITPPRRITLAAVL
                     ATLCYGVVPVLKKIGTDHGGPPVLGALVMHATGLMLLMTLGNLLKIERKWQRIPINSL
                     GCFVGAGFLYAIGSIFTLKALVHAPASVVAPIWSAQPIVSFFLAKTTLKGIEEVTFRD
                     GAAAALVVLGVLVLRLG"
     gene            1501..>2406
                     /locus_tag="VFY96_16680"
     CDS             1501..>2406
                     /locus_tag="VFY96_16680"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5608163.1"
                     /translation="MSANIGRDYFEIAAELKKRIEGDIRFDRYSRLLYSTDASIYQIE
                     PVGVVVPRHKGDVQAVIELAHKLDVSVLPRGGGTSLAGQSVGHSIVLDFSKYMQSVLE
                     VNQEELWCRVQPGLVQDELNAHVRGMGLQFGPDTSTSNRATIGGMIGNNSAGAHSLTY
                     GKTLDHVIELTVLLADGTEVVLRDFSPDAVESKSRTDTIEGRAYREVFRLAQQHKSEI
                     LARYPKIMRRVSGYNLDEFIKPKPFNLSRMLVGSEGTLATIVEAKMRLVPKPKWTAMD
                     VIHFNDDIEALQASQAILETAPYALE"
BASE COUNT          488 a          655 c          714 g          549 t
ORIGIN      
        1 gggattcggc gatccgggcg cgcgcgtatt gatcgtcgga ttggcgccgg cggcgcacgg
       61 cggcaatcgc acgggaagga tctttaccgg cgatcgcagc ggcgattggc tttacggcgc
      121 cctacatacc gccggatttg ccaatcagcc gaactccagt cacagaaacg atggtcttaa
      181 gcttagcgat tgctatataa cggcggcggt acgctgcgcg ccgccggaaa acaagcccac
      241 cttagccgaa tttcttcgct gtcgccccta tctcatagag gaactcaaat tgctcacggg
      301 gatcaaagtc gtgatcgtct tgggcaaaat cgcgttcgat gcctttctcg cggcgtacag
      361 tgggtgtggc ggtgttattc cgaaaccgcg ccccaaattc ggccacggcg ctgccgtcga
      421 tctcgcagac ggtccatgct tgatctgctc ctatcatccg agccagcaaa acacgtttac
      481 tggcaaactg acccgggcta tgttcgacaa tgttttcgct caggcaaggg ccgcgctcga
      541 taaatagctt tgtcgcatcc acatggaaat gggagttgtt tatgcgctct gcgctggagt
      601 tttctggggc acgagcccgg tgctggtcaa gcgagggctt gccggcgccg atgtcagcgc
      661 agcgacgctc taccagcagg cgacaattct cttgacgctt tgcttttccg cgttgctcga
      721 aggcgatctg ttcgccggga aaatacctgg gaaggcagtg ctcatatttg ccggcaccgg
      781 cgttgtcggc gcttacatcg gtcggacgtt gttcgtcaag agcgtcgatg agatcggcgc
      841 atctcgcgcg cagtcgctca ataactcctc gcccctaatt acagtcctgc tggcggccgt
      901 ggcgctcagc gagcggttat ctttggcggt tttgatcggc gtcattttta ttatttccgg
      961 cgtatttttc gtcactcgtg acgatgctac ggggttgggc aaaatcaccc caccgcggcg
     1021 gatcactttg gcggccgttt tggcgacgct ctgctatggc gtagtgccgg tgctgaaaaa
     1081 aatcggcacc gatcatggcg gtccgccggt gctgggcgca ctcgtgatgc atgcaacggg
     1141 gctgatgctg ctgatgacgc ttgggaattt gcttaaaatc gagcgtaagt ggcaaagaat
     1201 cccgattaac agtttgggct gtttcgtcgg cgcaggattc ttgtatgcta ttggttcaat
     1261 ttttactctg aaagcgctgg tgcacgcacc cgcatcggtg gtggcgccga tttggagcgc
     1321 gcaaccgatc gtcagttttt ttctcgccaa gacgactctc aaaggtatcg aagaagtgac
     1381 gtttagggac ggtgcagccg ccgccctggt ggttctcggt gtcctggtgt tacggttggg
     1441 ctgaagccat gttcaagtaa gggcgatctc atgtgatcgt ccgactatga ggagatcaca
     1501 atgagcgcca atatcggtcg tgattatttc gagatcgcag cggaactgaa gaaacgtatc
     1561 gaaggagaca ttcgattcga tcgctactcg cggctcctct attcgactga cgcctcgatc
     1621 tatcaaatcg aaccggttgg cgtggtcgtg ccgcgccata agggcgatgt gcaggccgtc
     1681 atcgagctcg cgcacaagct ggatgtctcg gttttgccgc gtggcggcgg cacgtcactg
     1741 gccgggcagt cggtcggcca ttcgatcgtg ctggattttt ccaaatacat gcagagcgta
     1801 ctggaggtta atcaagaaga actttggtgc cgcgtgcagc cgggtttagt gcaagacgag
     1861 ctcaatgcgc acgtgcgcgg catgggatta cagttcggcc ctgacacttc gacgtctaat
     1921 cgcgccacca tcggcggcat gatcggcaac aactccgccg gcgcccattc gctaacgtat
     1981 ggcaagactt tggaccacgt aatcgaactg acagtactgc tcgccgacgg caccgaggtt
     2041 gtgctgagag atttctcccc cgatgcggtc gaaagtaaaa gccgaactga cacgatcgag
     2101 ggcagggcgt atcgggaggt gttccggcta gcccagcagc acaagagcga aatcctcgcg
     2161 cgctatccga aaatcatgcg gcgtgtgtcg ggctacaatc ttgatgagtt catcaagccc
     2221 aagccgttca atctctcgcg catgctggtg ggctccgagg gcacactggc gaccatagtg
     2281 gaagccaaga tgcgcttggt gccgaaaccg aagtggaccg cgatggacgt cattcatttc
     2341 aacgacgaca tcgaagctct gcaagcatcg caggcgattc tggaaaccgc accctacgct
     2401 ttagaa
//
LOCUS       DASULZ010000812         2232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_602158, whole genome shotgun sequence.
ACCESSION   DASULZ010000812 DASULZ010000000
VERSION     DASULZ010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602158"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VFY96_16685"
     CDS             <1..791
                     /locus_tag="VFY96_16685"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5608164.1"
                     /translation="YSTPITVTLVSVTIHAVTLSAAVLLTGGIPTVSWWVLFLFAVTG
                     TLQPIIRLFTYAGIHYIGASRGTTVRGAHPLFSTALAIIFRGEQATLPVIGGTVLIVG
                     GITLISWQTEAQRAGFRWWYIGYPLGAAFLAGISHPLRRYALSLANEPLYLAAVIGVV
                     ALPWLASFVVLPRKGAEQPVWNRESIGAFLFAGVFETLGIVLVIAALSVGAVVIVSPI
                     VATSPLWILLGTWLFLRGIERLTVRTIIGALCVVAGTIAISLVR"
     gene            complement(1031..2185)
                     /locus_tag="VFY96_16690"
     CDS             complement(1031..2185)
                     /locus_tag="VFY96_16690"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX5608165.1"
                     /translation="MRQRSDYPIFSDREIATRHQAVYRLMEQANVDALLIYGAGRYAS
                     DVYWLSDWPSSREAYVLFQTAKEPVILLQLFNHFPMAKVMSVIKDVRWAGANTTSSVL
                     DLIRERGLDSKKIGIVGSLPYQVYLRIAEKFPGAALSDLSGKLRMMRTIRSAEEIERI
                     RFASKLTDESIEALAGGLKAGMREDEIAALIEPAYLKRGGYAGIHFMSSMPMRDPDFP
                     VPAQFQSSRKLRKGDCLITEISGAYSGYSGQIHRTFSVGEGPTPEWQQMHAAAVEAFE
                     ILSEVIKDGATTTEAEEAAEIIHRRGYSTYDDLVHGVNQYPPIFQTKTRKRHDSKEMV
                     FRENMVIVIQPNLITEDEKMGLQFGETLLVTKKGCETLNAYPREWVICQG"
BASE COUNT          422 a          639 c          610 g          561 t
ORIGIN      
        1 gctactctac cccgatcacc gtcacactgg tctcggtcac gattcatgcc gtgactttgt
       61 ccgccgccgt gttgctgacc ggcgggattc cgacggtatc ctggtgggtg ctgttccttt
      121 tcgctgtgac cggcacgctt cagccgatta tccggctgtt cacctacgcc ggtatccatt
      181 acatcggcgc gtcccggggc acgaccgtgc gcggcgcgca tccactgttt agcacagctc
      241 tggcaattat ttttcgcggc gagcaagcga ctctgccggt aatcggcggc acggtcctga
      301 tcgtcggcgg gatcacattg atctcatggc aaaccgaagc gcaacgcgcc ggttttcgct
      361 ggtggtatat tggttatcct ctcggcgccg ctttcctcgc cggaatcagc catccgctgc
      421 gccgttatgc gttgagtctg gccaacgaac ccttgtatct cgccgcggtt atcggcgtgg
      481 ttgcgctccc ctggctggct agctttgtag ttttgcccag aaaaggggca gagcaaccgg
      541 tctggaaccg cgaatcgatc ggcgcgtttt tgtttgccgg cgtgttcgaa acgttgggaa
      601 tcgttttagt gattgccgct ctgagcgtcg gcgcggtggt catcgtctcg ccgatcgtcg
      661 ccaccagccc cttgtggatt ctattgggca cctggctgtt cctgcgcggc atcgagcgtt
      721 tgaccgtgcg aacgattatt ggcgcgttgt gcgtcgttgc cggaacaatt gcgatatcgc
      781 tggtgcggta gccggagtat tcgtgtttta ttgctgcgga gaggtgaggc ttccaacgcg
      841 ccggcgatag ctttgcggct tgaggtgttc agcgcgccgc gccggtacaa cggccgcccg
      901 agaggatttt caaaccctcg tcaagcactc aaaaaagtca atggtccttc acgtcgggct
      961 gcggtcgccc cttccagcct cgcctttctt cctcgacagt ctccgtctag tatccagaga
     1021 attatttgta ctatccctgg cagataaccc attccctcgg ataggcattc aaagtctcgc
     1081 atcctttttt tgtgaccagc aaagtttcgc cgaattgcag ccccattttt tcatcctcgg
     1141 taataaggtt gggctggatg acaatgacca tgttttcgcg aaacaccatt tccttggaat
     1201 catgacgctt gcgggttttg gtttgaaaga tcgggggata ctgatttacg ccatggacca
     1261 gatcatcata ggtgctgtag ccgcgccgat gaatgatctc ggcagcctct tctgcttcgg
     1321 tggtggtggc gccatccttg atcacctccg acaggatttc gaacgcctca actgcggcgg
     1381 catgcatttg ttgccattcc ggcgtcggtc cttcgccgac ggaaaaggtc cggtgaatct
     1441 gtccgctgta acccgaatat gcgccgctga tctcagtgat caggcaatcg ccttttcgca
     1501 gtttgcggct cgattggaac tgcgccggca ccgggaaatc cgggtcgcgc attggcatgc
     1561 tgctcataaa atgaattccc gcgtaaccac ctctttttag ataagcaggc tcaatcagcg
     1621 cggcaatctc gtcctcgcgc atgcccgcct tgaggccgcc ggcgagcgct tcaatcgatt
     1681 catcggtgag cttggaagca aagcggatgc gctcgatttc ctccgcgctc ctgatggtcc
     1741 gcatcatccg caatttgccg ctaagatcgc tcaatgcggc gccgggaaat ttctcggcga
     1801 ttctaagata aacctgatag gggagtgaac caacgatgcc gatcttcttg gaatccaaac
     1861 cgcgctcgcg aatcagatcg aggacgctac tggtagtgtt ggcgccggcc cagcgcacgt
     1921 ctttaatcac ggacatcact ttcgccattg gaaagtgatt aaacaattgc agcagaataa
     1981 ccggttcttt agccgtttga aaaagcacgt aagcttcgcg gctgctcggc caatcactca
     2041 accaataaac gtccgaggcg tagcgcccag cgccgtagat gagaagcgca tcgacattcg
     2101 cctgttccat gagtcgataa accgcctgat gtcttgtggc gatctcccgg tcggaaaaaa
     2161 ttgggtaatc ggatcgttgc cgcatttgaa aatcctccta tcagtcgctc tgtctctgaa
     2221 tggatgactc gt
//
LOCUS       DASULZ010000813         3650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1514959, whole genome shotgun sequence.
ACCESSION   DASULZ010000813 DASULZ010000000
VERSION     DASULZ010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514959"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VFY96_16695"
     CDS             <1..559
                     /locus_tag="VFY96_16695"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX5608166.1"
                     /translation="TIDQYVCGKEKDPEDLLISANNGIKNAVVSLQNIAPDPKRDWNL
                     PPAKMDQKQCSFSPRVVVVPIGGTVEFLNSDRLLHNVRSAGKENPPFNRAQPHARSIS
                     FSFKQPELLRIDCDLHSWMRGWVVVAEHPFYAVTDEQGEFSLENVPRGKYTLQVWQET
                     LGTVTQQVVVGDKGITTASVTMGKK"
     gene            complement(569..1018)
                     /locus_tag="VFY96_16700"
     CDS             complement(569..1018)
                     /locus_tag="VFY96_16700"
                     /inference="COORDINATES: protein motif:HMM:NF013996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HEX5608167.1"
                     /translation="MAAVYTRRISLVLAIGLGFGLLISTETGRSWAGQEEQFTPEFRE
                     LRSSFDPKHTPKITAPESVKRGQWFDVTVDIGAGGEHPSLSEHAIRYIALYINTAEIA
                     RVYLHPVYSFPKVTFTIALDEGGSLRAIAEPNHSAGWEAAKKITVAP"
     gene            complement(1052..2104)
                     /locus_tag="VFY96_16705"
     CDS             complement(1052..2104)
                     /locus_tag="VFY96_16705"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5608168.1"
                     /translation="MSKFPRILAVDDNKQNLSLLEKALKTAGYEVLTAADGHAALELV
                     RSAGPDLVLLDVMMPGMSGYEVCQQIRANEATSLLPVVMLTALTDVTDRIRGIESGAD
                     DFLSKPVNREELLTRVRSLLRIKALHDEVETKNHLLRNLFGRYVSADVAAEIVADPRR
                     HLRLGGDKREVTVFFGDLRGFTPLAEQLDPQDVVDILNSYLKLVVDAVFEFRGTLDKF
                     RGDGFMAFFGAPIARDDDPLNAVRCALAIQERLARIAFVKFPEVRLHMGVGINTGIVI
                     AGNIGSERRTDYTVIGNEVNTAQRFESNAGPGQILITGNTYERVKHAVQVRELGRLRV
                     AGKQEGVEAYDVLSGV"
     gene            complement(2124..2498)
                     /locus_tag="VFY96_16710"
     CDS             complement(2124..2498)
                     /locus_tag="VFY96_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008382584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5608169.1"
                     /translation="MSGKTVLYVEDNEFNLKMVRQLLARTAYHLIEATDGELGVATAL
                     REIPDLILMDIQLPKLSGLDATRRLRADPKTAAIPIIVITSFALSGDDQKAKDAGATA
                     YMAKPYSPRELLQMIRKLLPEQ"
     gene            complement(2495..>3650)
                     /locus_tag="VFY96_16715"
     CDS             complement(2495..>3650)
                     /locus_tag="VFY96_16715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5608170.1"
                     /translation="NLIRDGKVADAMRLHQEKQYPLYQRIEEFVTQLVLRERNKMDQL
                     RASVAAANRSTLILMAISAVALMLVALLLGFVISWSFILPVQKAYGFVSEVAKGNFAA
                     TINVPNRDEFGALAAHMNHMSQELHRLSEVQHRASQELQRLNERLQQASKAKSNFLAN
                     MSHELRTPMNAILGFVEMMLDEIYGEVPPQLREPLTDVQTNGKHLLRLINDVLDLSKI
                     EAGRMELVLADYSVQDVVETIRTSLQSLALEKGLEFVAAAGDDIPLAFGDGKRITQCL
                     MNLVGNALKFTKHGRVAVAVEQQGKDLLYRVSDTGIGIPKEELENIFGEFQQVDTAIT
                     REFSGTGLGLNISKKFVEMHGGRIWVESELGAGSTFFFTVPLRVETSEVT"
BASE COUNT          791 a         1031 c         1025 g          803 t
ORIGIN      
        1 gactatcgat cagtacgtct gcggcaagga aaaagatccg gaagacctgc tgatttccgc
       61 caacaatggg atcaaaaacg cggtcgtatc gctgcaaaac attgcgccgg acccaaagcg
      121 cgattggaat ctgccgcccg cgaaaatgga tcagaagcaa tgctcgttca gtccacgcgt
      181 ggtggtcgtg ccgataggcg gaacggtgga atttctcaac agcgaccgct tgctgcacaa
      241 cgtcaggagc gccgggaaag aaaatccgcc gttcaatcgg gcacagcccc atgcgcgaag
      301 catttcattt tcgttcaaac aacccgagct attgcgtatt gattgcgatc ttcattcctg
      361 gatgcgtggc tgggtggtcg ttgccgagca tccgttctat gccgtgaccg atgaacaggg
      421 cgagtttagc ctggaaaatg taccgcgagg aaaatacacg ctgcaggtct ggcaggaaac
      481 tttgggaact gtgacccagc aggtggtggt gggagacaaa gggattacca cggcatcggt
      541 caccatgggc aaaaaataga cctgctggtt acggcgcgac ggtgattttt tttgccgctt
      601 cccaaccggc cgaatggttg ggttcagcga tcgccctaag cgagcctcct tcgtcgagag
      661 cgatggtaaa cgtgactttc gggaacgaat agaccggatg gagataaacg cgcgcgatct
      721 cggcggtgtt gatatagagc gcgatgtacc ggatggcgtg ctcgctcagg gatggatgtt
      781 ctccgccggc gccgatatcg accgtgacat caaaccattg gcctcgtttc acggattccg
      841 gggcggtgat tttcggcgta tgcttgggat cgaaggagga gcgcaattcc ctgaactccg
      901 gggtgaactg ctcttcttgc ccagcccacg agcggccggt ctcagtggag atcaacagac
      961 cgaaaccaag tccgatcgcc aggacaagcg agattcgcct cgtataaaca gctgccattc
     1021 ttaattcctc cgtgatgaat ttcggggcta gctatacacc gctgagcacg tcgtaagctt
     1081 caacgccttc ctgtttaccc gctactcgca atcgcccaag ctcgcgcacc tgcacggcgt
     1141 gtttcacccg ctcgtaggtg ttccccgtga tcaaaatctg cccgggcccg gcattggatt
     1201 caaaccgctg ggcggtgttc acctcgttgc cgattaccgt gtagtcggtt cttcgttcgg
     1261 aaccgatgtt tcccgcgatc acaattcccg tgttgatgcc gacccccatg tgcaggcgta
     1321 cctcgggaaa ttttacgaaa gcgatgcgcg ccaatctttc ctgaatcgcc agcgcgcagc
     1381 gcaccgcatt taaaggatca tcgtcgcgcg cgatgggcgc gccgaaaaag gccatgaatc
     1441 catcgccacg aaatttgtcg agggttcccc tgaactcaaa gacggcatcg acgaccagct
     1501 tcaaatagct gttgaggata tccaccacat cttggggatc gagttgttcg gcgagcggcg
     1561 tgaatccgcg aagatcgccg aaaaaaaccg tgacttcgcg cttgtcaccg cccaatctca
     1621 gatgccgccg gggatcggcg acgatttcgg cggcgacgtc cgcagacacg taccggccga
     1681 aaagattacg gagcagatga ttttttgtct cgacctcgtc gtggagcgct ttaatcctga
     1741 gaagcgatct gactcgcgtc aacagctctt cgcggtttac cggtttactg agaaaatcat
     1801 cggcccctga ttcgatgccg cgtattcgat cggtcacgtc ggtaagcgca gtcaacatca
     1861 ccacgggaag gagggaggta gcttcattgg cgcggatctg ctgacaaact tcgtatcccg
     1921 acatacccgg catcatgaca tcgaggagga cgagatctgg ccccgcgctg cggacgagct
     1981 cgagcgcggc gtggccgtct gccgccgtaa gtacctcgta accggccgtt ttgagagctt
     2041 tctcgagcaa agaaagattt tgcttgttgt catcgacggc cagaatgcga ggaaatttgc
     2101 tcacgatgaa ccagctcgat cgcttattgc tcgggaagca gcttgcggat catctgtaac
     2161 agctcgcgcg gactgtaggg ctttgccata taggcggtcg cccccgcatc cttagctttc
     2221 tgatcatcgc cgctgagcgc aaaggaggta atcacgataa ttggaatagc ggcggtcttg
     2281 ggatccgcac gaagccggcg ggtggcgtca aggccggata attttggcag ttggatgtcc
     2341 atcaggatca ggtcaggaat ttccctcagc gctgtcgcca caccgagctc accatccgtc
     2401 gcttcgatca gatgataagc cgtgcgcgcg agaagctgcc ggaccatttt cagattgaac
     2461 tcgttatctt cgacatacag aacggtcttg ccgctcatgt gacctcgcta gtttccacgc
     2521 gcaaagggac ggtaaagaaa aaagtcgatc ctgcacccag ctcgctttcg acccagatcc
     2581 ggccaccgtg catttcgacg aattttttgc tgatgttcag cccgagtccg gtgccgctga
     2641 actcccgggt gatcgcggta tcgacttgtt ggaattcccc gaagatgttt tccagctctt
     2701 ctttgggaat gccaatgccg gtatccgata cccgatagag caggtccttt ccctgctgct
     2761 cgacggccac cgccacccgg ccgtgcttgg tgaatttcag cgcgttgccg acgaggttca
     2821 tcagacactg ggtgatccgc ttaccgtcgc caaacgcaag cggaatatcg tccccggcgg
     2881 cggcgacgaa ttccagacct ttttccaaag cgagcgattg cagcgacgtg cgaatggttt
     2941 cgacgacgtc ctgaaccgaa taatccgcca aaaccagctc catgcggccc gcctcgatct
     3001 tcgagagatc gagtacgtcg ttgatcaggc gcagcagatg cttgccgttg gtctggacat
     3061 cggttagcgg ctcgcgcaac tgcggtggaa cctcgccgta aatctcatcg agcatcatct
     3121 cgacaaaacc gagtatcgca ttcatcggcg tacgcagctc gtgtgacatg ttggctagaa
     3181 agttcgattt agccttgctc gcctgctgaa gccgctcgtt cagcctctgg agctcctgcg
     3241 acgcccgatg ttgcacttcg gagagacggt gcagctcctg gctcatgtga ttcatatgcg
     3301 ccgcgagggc gccgaactcg tcgcggttag ggacgttgat cgtggccgca aaatttcctt
     3361 tcgccacctc gctgacgaaa ccataggcct tctgaaccgg tagaatgaac gaccatgaga
     3421 tgacgaaccc caatagtaat gccacgagca tcaaagccac cgcgctgatc gccataagaa
     3481 tcagtgtgct tcggttggcg gctgcgacac tggcgcgaag ctggtccatt ttgttgcgct
     3541 cgcgcagcac caattgcgtg acgaactctt caatgcgctg gtagagcgga tactgcttct
     3601 cctggtgcag tcgcatggca tcggcgactt tgccgtcgcg aatcaggttt
//
LOCUS       DASULZ010000814         4984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_195271, whole genome shotgun sequence.
ACCESSION   DASULZ010000814 DASULZ010000000
VERSION     DASULZ010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195271"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..1032
                     /locus_tag="VFY96_16720"
     CDS             217..1032
                     /locus_tag="VFY96_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5608171.1"
                     /translation="MDFIRPSDVDAHLRRLDRHKIPHSDPIRTAADGDEGTLIFFADP
                     DGNQYEFWAPENMPEGAMEIATECHVGRISHAVLGARDLLRTADFFENYCGVKAEQNS
                     KTVEGTLVLRMRAGGRLIYKLVAKVDERVSGHGNWWDMHTALTVRDEEFLPSYRRMWA
                     GIPEEKGYKADLNLSIEQQEALPARTGLHRSPVGIRWKEICDRGDEFYDWDCHSFHFI
                     GGRPLKPDGSMALYEAIEQEHYLQDLAASLNKGSEQGLLEGLGRENPFKSRDG"
     gene            complement(1077..2714)
                     /locus_tag="VFY96_16725"
     CDS             complement(1077..2714)
                     /locus_tag="VFY96_16725"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5608172.1"
                     /translation="MAEKIVVVQGGKLIDGTGRPPVEDSIIVIAAGRIQTVGAAGEVA
                     IPEGADIVDVKGRTVLPGFIDGHGHLEDFHGELYLHLGITTCANIELYQDGPWTRAQK
                     EGTALGKIRGPRIWMSGRAIGGVSTGHDAFGSRTARDNIIVTTPDEVRRAVQRKRELG
                     CEVLKVNEFLSLDLVKVACDEAHRLGMPVAAHSWDAVGSSKAGVDAIEHIWSVGYSSI
                     PYPPARRKLAEDRLGGVIDQELAGAYYQTENYGAVIDAMAEHQVAWTPTIAKWLRPLS
                     PSAERFRQRESQILNDRNAELPAAVRAVTENSYEKLWKRYSPAQQEQARVGYDKANEF
                     IRRFVEAGGILKEGSDPPRGMAALLMHQALVMDVEAGVPPMKAIQAATLNVARTFKKD
                     KDYGSVEPGKVADLSIIEGDPLADIWMTQNVKMVIMDGKVIDSGFSKYKNPIPSFYSY
                     QSLPLDLHISPQFLIQGSGPTTLRVRGEGGMWPFHQVVLNGNPLPTSFVSKNELKATI
                     APEVISAAGTYLVTLKCEGESFPESHRAHLVVGFSDR"
     gene            3009..3647
                     /locus_tag="VFY96_16730"
     CDS             3009..3647
                     /locus_tag="VFY96_16730"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX5608173.1"
                     /translation="MKQNRLLIAGSVLAAAWMTTGTAMAQSSGSGDTGRSSSGSAQST
                     KEVHPDVNTSKGQSKSERADETGTPLPKGSPYAGTVEKGKSSSADSGMDTSTSPSARG
                     SSSASRMARSGSAANIKEAQQALKDKGYDPGPVDGVMGSKTKEAIKSFQSASNLQATG
                     TLDAQTADKLGVQSGSSSSRSSSSSSRDNMKSSNTTVGKDTDQPNQTPSKNR"
     gene            complement(3799..>4984)
                     /locus_tag="VFY96_16735"
     CDS             complement(3799..>4984)
                     /locus_tag="VFY96_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX5608174.1"
                     /translation="ALIDTFIRESTEYRNAVEAGSSPETVLRDLRNNPKFMAKLRSDK
                     TRLQAGFVAIDPLTGYVKAWVGSRDFKTDQFDHVIQARRQPGSTFKPFVYGAAMEQGM
                     SPDKRFIDRAVEIALPDGTMWRPSDLSQPTGEPMTAREGLVLSKNTITVQVMQEVGPK
                     RTADLARRMGVNQSNLDEVPSLALGTSPVTPFEMVSAYSTLAARGEYRTPIFITRITD
                     KNGKVLEHFENQSRRVLSDKTVGNLINMMRDAVDRGTGQAVRTQFGVRADVAGKTGTT
                     QKNSDGWFILMHPRLVAGAWVGFNDSRITMRSNYWGEGGHNAILLVGDFFRHILSAQF
                     IDPSAQFPYARPREFFWDPFLDRLKDWWFGESAAPAPVRPSRRAEGDLSDTSSARINA
                     EA"
BASE COUNT         1063 a         1453 c         1382 g         1086 t
ORIGIN      
        1 tcggcaccaa tgacatggtt gcctgggtag attgggcggt aaacgccacc ggaattacac
       61 gccgtccgat catcggactc acgactgccg cgcgaaaacg caacatcaag atcacgagct
      121 tcctgtggtg ggagggtggt tcctgtagga tcggcgcttt tctccagcct gaaatctacc
      181 cgcctgcgaa gcctttagga gaggatctgc cgcgctgtgg attttatccg gccgagcgat
      241 gtagatgcgc atctgcggcg gctcgatcgg cacaagattc cgcatagtga tccgattcgt
      301 acggctgccg acggcgacga gggcacgttg attttctttg ccgatcccga cggcaatcag
      361 tacgaatttt gggcccccga gaacatgccc gagggcgcca tggaaattgc gacagaatgt
      421 cacgtcggcc ggatcagtca cgcggtgctc ggcgcgcgcg atctccttcg aaccgccgat
      481 ttcttcgaaa attactgcgg tgtcaaagcc gagcagaatt ccaaaaccgt tgagggcacc
      541 ctcgtgctgc ggatgcgtgc aggcgggcgc ctgatctata aactggtggc caaagtcgac
      601 gagcgtgtct caggccacgg caactggtgg gatatgcaca ccgcgctcac ggttcgtgac
      661 gaagaatttc tgccttccta tcgtcgcatg tgggcgggga ttcccgaaga aaaaggctac
      721 aaagccgatc tgaatttatc catcgagcaa caagaggctt tgcccgcgcg cacggggttg
      781 catcgcagtc ctgtcggcat caggtggaaa gagatttgtg atcgcggcga tgaattttac
      841 gactgggact gccactcctt tcattttatc ggcggcaggc cgctcaagcc ggacggctcg
      901 atggcgcttt acgaggcgat tgagcaagaa cattatctgc aggacctcgc ggcttcactg
      961 aacaagggga gcgagcaggg tctcctggaa ggattgggcc gcgaaaaccc gtttaagtcc
     1021 cgcgatgggt aacccgtggc acgtgcttcg tcccgcttct tgcgttccat cgggactcat
     1081 cgatcggaaa atccgacgac caagtgggcg cggtgcgatt cgggaaacga ctctccctca
     1141 cacttgagcg tgaccaggta cgttcccgcg gcggaaatca cctccggtgc aatggtcgct
     1201 ttcagttcgt ttttggaaac gaagctggtc ggcaatggat tgccattcag cacgacttga
     1261 tgaaagggcc acattccacc ctcacctcgt acgcgcaagg tcgtcggccc ggagccttgg
     1321 attaggaact gcggcgaaat atgcaggtca aggggtaagc tttgatacga gtagaatgaa
     1381 ggaattggat tcttgtactt gctaaatccg ctgtcgatga ccttgccgtc catgatcacc
     1441 atcttcacgt tttgagtcat ccagatgtct gccagcggat cgccttcgat aatcgataaa
     1501 tccgccacct tgcccggctc gacgctgccg tagtctttgt ccttcttgaa ggtccgcgcg
     1561 acattgagcg ttgccgcttg aatcgctttc atcggcggca cacccgcctc gacgtccatc
     1621 acaagggcct gatgcatcag caaggccgcc atcccgcgcg gcggatcgga gccttccttc
     1681 agaatgccgc ccgcctcaac gaaccggcga ataaattcgt tggccttgtc ataaccgacc
     1741 cgggcctgct cctgttgtgc cggggaatag cgcttccata atttctcgta cgagttctcg
     1801 gtcaccgcgc gcacggccgc gggcagctcg gcattgcggt cgttcagaat ttgactttcc
     1861 ctttgtctga atcgctcggc gctcggcgat agcgggcgca gccacttggc gatagtcggc
     1921 gtccacgcga cctggtgttc cgccatggcg tcgataaccg cgccgtagtt ctccgtttgg
     1981 taataggccc ccgcgagctc ttgatcgatt actccgccga gcctgtcttc cgcgagtttg
     2041 cgccgcgcag gcggataagg gatcgagctg tatccgaccg accagatatg ctcgatggcg
     2101 tcgacgcccg ctttcgaaga gcccaccgcg tcccagctgt gcgcagccac cggcattcct
     2161 agacggtgcg cttcatcaca agcgactttg accagatcca gggaaagaaa ttcattgact
     2221 ttcagtacct cacaaccaag ctctcgcttt cgctgcacgg cacgacgtac ctcgtcaggc
     2281 gttgtgacaa tgatgttgtc gcgcgcagtt ctggagccga aagcatcgtg accggtgctg
     2341 actccgccaa tcgcccgacc tgacatccaa attcgcgggc cacgaatctt ccccagtgca
     2401 gttccctctt tctgcgcccg ggtccagggc ccatcctgat agagctcaat attcgcacag
     2461 gtggttattc ctaggtgtaa atagagctcg ccgtgaaagt cctccaggtg gccatggcca
     2521 tcgatgaatc ccggcagaac ggttctgcct ttgacatcga cgatgtccgc gccttcggga
     2581 attgcgacct cgccggcggc tccaaccgtt tgaattcgcc ctgctgcgat gacgattatg
     2641 gaatcctcca cgggcggtct gcctgtgccg tcgatcaatt ttccgccctg cacgaccacg
     2701 attttttccg ccatgttctc ctcggcgctc acctgaactg aattatttat cttctttctc
     2761 ttggcgctgc gcaatccgac ttggtgctga ttgctgattg gccatgttta ttgatgaaga
     2821 cgttttctcc gacagggaga ggaaaaatcc tcccgcccaa tcgaaacctt acgccatttt
     2881 ttcataaaaa ctgcgctgct cgcagcaatt attctcagct ccgggacaaa acacaccgtc
     2941 atcgtctaaa tcctctgggc atgacgattg cgctactatt ggagcagtac atactaagga
     3001 ggaggcttat gaaacaaaat cgattgttga tagcaggctc tgtgctcgct gcggcctgga
     3061 tgacgactgg gacagctatg gcacaatcgt ccggctcagg cgatacgggg cgcagcagta
     3121 gcggttcggc gcaatccacc aaagaagtgc atccggacgt aaatacttcg aagggccaat
     3181 cgaagtcgga gcgagctgac gaaaccggga cgccgttacc gaagggcagc ccctatgctg
     3241 ggactgtcga aaaagggaag agcagtagcg cggattccgg catggacacc agcacaagcc
     3301 caagtgcccg tgggagttcc agcgccagcc ggatggctcg cagtggttcc gcggccaata
     3361 tcaaagaagc tcaacaagct ttgaaggata aaggttacga tccggggccg gtagacggag
     3421 tgatgggtag taagacaaaa gaagcgatca aatcgtttca gagcgcaagc aatctacaag
     3481 cgactggaac cctcgatgca cagactgccg acaagctcgg cgttcagtcg ggtagctcca
     3541 gcagccgatc cagctcttcg tcttcgcgcg acaacatgaa atccagcaat acaaccgtcg
     3601 gaaaagatac cgatcagccg aaccagaccc cttcgaaaaa ccgctaagct cacctcagcc
     3661 ggttagggcg cctctgtgtc gttagacgca cggcggccct aaccgcgcgc cattgccggc
     3721 cttctcggcg agaaagatgc ctgaatctgg aagctaaaat cttgaggcca tactgcctcc
     3781 aggacttgtt cgcagccgct aggcttccgc gttaattcgc gccgacgacg tatctgatag
     3841 atccccttca gcacgccgcg acggccgaac tggtgctgga gccgcgcttt cgccaaacca
     3901 ccagtctttt aaccggtcca ggaacgggtc ccaaaaaaat tcgcgcggcc tcgcgtacgg
     3961 aaactgagcg ctgggatcga taaattgcgc actgagaatg tgccgaaaga aatcgccgac
     4021 tagcaaaata gcgttgtgtc cgccttcgcc ccaatagttg ctgcgcatcg taatccgaga
     4081 gtcgttgaag ccgacccatg ctcctgcgac caagcgtgga tgcatcagga taaaccatcc
     4141 atcgctgttc ttttgggtcg tcccggtctt gccggccaca tcggcgcgca cgccgaattg
     4201 tgttcgcacc gcctgccctg tgccgcgatc gacggcatcg cgcatcatgt tgatcaaatt
     4261 tccgacggtt ttgtccgaca gaacacgcct gctttggttt tcaaaatgct cgagcacctt
     4321 gccgttttta tcggtaatgc gggtgataaa gatcggcgtg cgatattcgc cgcgcgcggc
     4381 gagcgtgctg taagctgata ccatttcaaa tggtgtaacc gggctggtgc ccaaagccaa
     4441 ggaaggcacc tcgtcgagat tgctttggtt cacacccatt cggcgcgcca agtccgcggt
     4501 tcttttcggg ccgacttcct gcatcacctg cactgtaatg gtattcttcg agagaactag
     4561 tccctcgcgc gcggtcattg gctcgccggt aggctgggac aaatcggaag gacgccacat
     4621 tgtgccatcg ggaagcgcaa tctccaccgc gcgatcgata aaccgcttgt ccggtgacat
     4681 gccctgctcc atggcggcac catagacaaa cggtttgaag gtcgagccgg gttgccggcg
     4741 cgcctggatc acgtgatcga attgatcggt cttgaaatca cggctcccga cccaggcttt
     4801 gacgtaaccg gtgagcggat caatcgccac aaagccggcc tgtaaacggg ttttgtcgct
     4861 gcgcagcttg gccatgaatt ttggattgtt tcgcaaatcc cgcagtacag tttcgggact
     4921 cgagcccgcc tcgaccgcgt ttcggtactc cgttgattcg cgaataaacg tgtcgatcaa
     4981 agcg
//
LOCUS       DASULZ010000815         3374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_804531, whole genome shotgun sequence.
ACCESSION   DASULZ010000815 DASULZ010000000
VERSION     DASULZ010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804531"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..1058)
                     /locus_tag="VFY96_16740"
     CDS             complement(198..1058)
                     /locus_tag="VFY96_16740"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5608175.1"
                     /translation="MNEAGYKGFRQDELEYQYNPRVCVPEYPELAKVRAAQARKVRES
                     AKSWLNIAYGASPRERLDIYAANKPAGPVLIYIHGGYWRSGSKEDNCNFVPTFTERGA
                     TVVLVEYDLCPNVTVTDIVRQTRSAIAWTYKNILRYGGDPAKLFVFGHSAGGHLTAMA
                     LAHDWRQDGLPENLIKGAVATSGVYDLDMVMKISVQEEVRMTAEVAARNNPLWHPPRV
                     KCPLIVAVGANEPEGWQQMSKDYFDFCQRTGVPVDYLVEPNANHYTMTEHLLDANRPL
                     TQAMIKQLGI"
     gene            complement(1224..1499)
                     /locus_tag="VFY96_16745"
     CDS             complement(1224..1499)
                     /locus_tag="VFY96_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HEX5608176.1"
                     /translation="MAAAAKKKRKPNAAFMKPVQPDTALSAVVGNKAIPRTEVTKKLW
                     AYIKKNGLQDKKKRTNINADDKLKAVFGGKKTVTMFEMTKLVSKHVK"
     gene            complement(1575..>3374)
                     /locus_tag="VFY96_16750"
     CDS             complement(1575..>3374)
                     /locus_tag="VFY96_16750"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX5608177.1"
                     /translation="PIRPSYCSEFPLRLTSDASRLLMTVDPITLQVIQARLAGIVQEM
                     QNSLFRTGYSTIIRESQDASCAILNTAGEVVAQHVVLPLHMGAFPACAAAVLKSFVSD
                     DIYEGDAFIINHPYLGGSPHAPDMGVLTPIFYRGEWVGFAANMAHKSDIGGTVPGSGS
                     GTAREIYQEGLHLPAVKFMSRRQPVKEIDAVITANSRTPDVVIGDLRGQVGAARLGER
                     RIVELMDRYGKATLLESTELLSDYTENRVRQTIASWPDGESAGESFVDHDGIDLTRPI
                     RIQVKISKHGDKLHFDFSGSSDQTQGPANIRPPLVRAACAYCLIALVDRFLPINQGLA
                     RIIDATFRPGSVVDPRFPAAVNTYMPTALTVVEAVLNALARFVPDKQIAGGSGSAALV
                     LGGRTESSERAFVHYEIFSGGTGARSGKDGVSATAFHLSNCRTAPIEIIESEFPARVE
                     RFEIIADSGGAGEWRGGLGFARDYRILADEVRFSMRTDKHAIEPFGSASGLPGRKGAC
                     VVNPESKDEKRLPSRFGDYRLRRHDIVRLERPGGGGLGDPLRRPMEKVLEDVRQGYVS
                     VERARSDYAVAVRWTDGQPELDSAETAFLRGKK"
BASE COUNT          707 a          966 c          942 g          759 t
ORIGIN      
        1 ttctcgcttc ctcgaaatga caggaggcaa ctggcgaaac acgggatcta ttcggtgtgc
       61 tccgaatttc cgagtagcgc gaagcgcgta ccgttcggta cggtagcgct gctcgctgcc
      121 tactcaggac gtttggatac atcccgttac tttaatagtg cgaagtgagt ccaggaaccg
      181 cgataacgat ttcgtcttta gatccccaat tgcttgatca tcgcctgggt gagcgggcga
      241 ttggcgtcga gtaaatgctc ggtcatcgtg taatggttcg cgttcggttc cacgaggtaa
      301 tcgaccggca cgccggttcg ttggcagaaa tcgaaatagt cctttgacat ctgctgccaa
      361 ccttcaggct cgtttgcgcc gaccgcgacg atcagcgggc atttgactcg aggcggatgc
      421 cataacggat tatttcttgc agcaacctcg gcggtcatgc gcacttcttc ctggacgctg
      481 atcttcatga ccatatctag atcgtaaact cctgaagtgg cgactgcgcc tttgatgaga
      541 ttctcgggca gtccgtcctg tcgccaatcg tgcgcgagcg ccatcgcggt gagatggcca
      601 ccggcggaat gaccaaagac gaaaagtttc gcgggatcgc ctccgtatcg aagaatgttc
      661 ttgtaggtcc aagcaatcgc tgaacgggtc tgacggacga tatccgtgac ggtcacattt
      721 ggacagagat catactcgac caggaccacc gtggcgccgc gctcggtgaa ggtcggcaca
      781 aaattacaat tgtcttcctt gctgccgctg cgccagtagc ccccatgaat gtagatgagc
      841 accggtccag ccggtttgtt agcagcgtaa atatccaatc gctcccgcgg cgatgcgccg
      901 taagcgatgt tcagccacga tttggcgctc tctcgtactt tacgggcttg tgcggcacga
      961 accttcgcga gctcgggata ttcaggcacg cagacacgcg ggttgtactg atattccaac
     1021 tcgtcctggc gaaaaccttt gtaaccagct tcgttcatgt gaatgagcct cagtagatta
     1081 cttgcacggg ccccacacta gtggaagctg cgcttgcgtg tcaatgacgg gttgaaagga
     1141 cgagcggttt agaaaaacga aaaggccaag agcgcccgcg ccgcaggacc cttggccttg
     1201 cctatgaact gtaacggaga tgcttacttc acgtgtttgc tgacaagctt cgtcatctcg
     1261 aacatcgtga cggtcttttt gccgccgaat acagctttca atttatcatc ggcgttgatg
     1321 ttggtccttt tctttttgtc ttgcaggccg ttcttcttga tgtacgccca caatttcttc
     1381 gtgacctcag tgcgcggaat cgccttgttc ccgaccacgg cgctcagagc ggtgtccggt
     1441 tgcaccggtt tcatgaatgc cgcattcggt ttgcgtttct tttttgctgc tgctgccatt
     1501 cggtttcctc cttcagaaaa gaaaatattt aggtacgtgt atagcagacg cccgtggcta
     1561 ttgccaatgt ttttttactt ttttcctcgg agaaacgcgg tttccgcact gtccagctcc
     1621 ggttgcccgt ccgtccagcg gacggcgacc gcataatcgc tgcgcgcccg ttcgactgac
     1681 acatacccct ggcgcacatc ttccaagacc ttctccatag ggcgtcgcag cgggtcaccc
     1741 aggccgccgc cgccgggacg ttccagtcga acgatgtcat ggcggcgaag ccggtaatca
     1801 ccgaaacgcg aggggagtcg tttttcatct ttgctctcgg gatttacgac gcaagcgcct
     1861 ttgcgcccag gaaggccgct agcactgcca aagggctcaa tcgcgtgctt atcagtgcgc
     1921 atggaaaagc gcacctcatc ggcgagaatg cggtagtccc gcgcgaatcc aagcccgccg
     1981 cgccattcgc cggcgccgcc cgagtcggcg attatctcga aacgctccac ccgcgccgga
     2041 aattccgatt cgataatttc aatcggcgct gtcctacaat tactcaaatg aaacgccgtg
     2101 gccgagactc cgtctttacc cgagcgcgcc cctgtgccgc cgctaaaaat ttcgtaatgc
     2161 acaaacgccc gttcgctcga ttccgtgcgg ccgccgagca ccaacgccgc gctaccggag
     2221 ccgccggcga tctgtttgtc cggaacaaaa cgggcgagag cgttcaacac cgcctccacg
     2281 acggtgagcg cggtcggcat ataggtattt acggcggcag gaaagcgtgg atcgacaacg
     2341 cttcccggac ggaaagtagc atcgattatc cgagcgagtc cctgattgat ggggagaaaa
     2401 cggtcgacca gtgcgatcag gcagtacgcg caagcggcgc gcaccagcgg cggacgaata
     2461 tttgccgggc cctgagtttg atcgctgctg ccgctaaaat cgaagtggag cttgtcaccg
     2521 tgtttactga tttttacctg aatccgaatc gggcgagtaa ggtcgatgcc atcatgatcg
     2581 acgaagctct ctcccgcgga ttcgccgtcc ggccatgatg cgatcgtctg gcgcacgcga
     2641 ttttcagtgt agtcggataa tagctcagta gactcgagaa gggtcgcctt gccgtagcga
     2701 tccatcaatt cgacgatccg tcgctcaccc aaccgtgcgg cgcctacctg accgcgcaga
     2761 tcgccaatca caacatcagg ggtacggctg ttcgccgtga tcaccgcatc gatttctttg
     2821 accggctggc gccgggacat gaatttgacc gccggtagat ggagtccttc ctgataaatt
     2881 tcgcgagcag tgcccgatcc gctgcctgga acggtgccgc cgatatcgct cttgtgcgcc
     2941 atgttcgcgg caaaaccgac ccattcgcct cggtaaaaaa tcggtgtcag cacccccata
     3001 tccggcgcat gcggactgcc gccgagatac gggtgattga tgatgaacgc gtcaccctcg
     3061 taaatgtcgt cggacacaaa gctcttcagc actgcggcgg cgcaagcggg aaaggcgccc
     3121 atatgcaggg gcaacacgac gtgctgtgcg accacctcac cggcggtatt caaaattgcg
     3181 cagctggcat cctgggattc ccgaatgatg gtcgaatagc cggtgcggaa aagcgaattc
     3241 tgcatttcct gcacaatacc ggcgaggcgc gcctggatga cctggagtgt gatgggatcg
     3301 acagtcatga gaaggcgtga ggcgtcagat gtaagtcgta aggggaattc ggaacaataa
     3361 gacggtctaa tcgg
//
LOCUS       DASULZ010000816         6808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1007371, whole genome shotgun sequence.
ACCESSION   DASULZ010000816 DASULZ010000000
VERSION     DASULZ010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6808
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007371"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VFY96_16755"
     CDS             <1..924
                     /locus_tag="VFY96_16755"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HEX5608178.1"
                     /translation="TQASLIKELDERGIGRPSTYAAIISNILDREYVIQNEGRSLAPT
                     DLGFLVTDLLVESFPDILNVEFTAGMEDELDKIEDGKEKWTKAMKRFYTPFSRDLKKA
                     EKQMRDVKRQEVPTDINCDKCGAKMVIKWGRNGEFLACPQYPECKNTKNFKREDNGGI
                     EIVADEEVNEACELCGRPMLLRWGKFGKFLGCSGYPECKNIQPLEKPVDLGIKCPECK
                     EGNIKERKSRWGKIFYGCDRYPECKFASWDKPIAIACPDCGSPILLEKLTKRAGRVQR
                     CYNKECGYSVQVEAPLSTKKPPADHSSAASA"
     gene            986..2329
                     /gene="trmFO"
                     /locus_tag="VFY96_16760"
     CDS             986..2329
                     /gene="trmFO"
                     /locus_tag="VFY96_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017427921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEX5608179.1"
                     /translation="MSDPVCIIGGGLAGAEAAWQLARRGVAVELYEMRPQRSTDAHAG
                     SDLGELVCSNSLRSDLLSAPAGLLKAEMRRLDSIIVRCAEQHRVPAGSALAVDRDAFS
                     AGLTEIVQELPGVRIIREEVREIPDEGVVIVATGPLTSPALSGQLAAALGEKHLYFYD
                     AISPIVTADSIDMELAFRAARYGKGGDDYLNLPLTREEYYRFVDGLLAAERVPTHAFE
                     RFVAFAGCMPIEEMADRGKDTLSFGPMRAVGLVDPRSGKRPHAVVQLRQENRARTLYN
                     LVGFQTKMTYSEQRRVFALIPGLGKAEFVRLGSMHRNTFINAPLHLEPTLQWRRRPTL
                     FFAGQMTGVEGYIESGATGMLAGINAARLLAGDAPVVPPITTALGSLLAYITDCERKQ
                     FQPMNVNFGLMPPLAKRLRGKAKKEMMSRRALADLTAWAGETGLIPSSTETVAVS"
     gene            2326..3270
                     /locus_tag="VFY96_16765"
     CDS             2326..3270
                     /locus_tag="VFY96_16765"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX5608180.1"
                     /translation="MSPRRSAFFDIGTNTILCLIAEIRDTGRFRVLDDLAEIVGLGQG
                     VDQTRLISAEAARRASNVLQRFVHQCRSLGVEEMTAVGTSALRDANNSEQVRSRFRDE
                     IGLDVRVLSGAEEAAYSFLAVQRGLPLANRELLVIDIGGGSTEFIRGNDSGVLRAISI
                     DMGSMRLTERFLHSDPVSPAEAETMITAIDRELAALPSRGIDASSAVALVGIAATFTT
                     LAAMEKRLVTYSHPEIHGSRITAGQVRAQVSSLQMKTIAQRKGLIGLDPKRAEVIFAG
                     ACLIDRIMSFYQQDEVVVSDQGVRYGLLHEAAEAMPKG"
     gene            3370..3639
                     /locus_tag="VFY96_16770"
     CDS             3370..3639
                     /locus_tag="VFY96_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX5608181.1"
                     /translation="MTKAELVEAVAKSAKISKVTATEAVDATFDHIGRAIRKSKRFQV
                     PGFGTFTVRSRKARKGRNPQTGAVINIKASRSVGFKPAPVLKNGL"
     gene            3749..4666
                     /locus_tag="VFY96_16775"
     CDS             3749..4666
                     /locus_tag="VFY96_16775"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX5608182.1"
                     /translation="MSLKTESIWMDGELVRWEDAKVHVLTHALHYGTAYFEGIRCYLL
                     TDGRSAVFRLAEHTRRLVDSGKILGFPIPYTEEQLNRATLDVIRANKLKECYIRPLAF
                     VGLGELGLFAPKNPVSVCIAVFPWGAYLGDEGLRNGIRAKVSSFTRHHVNVMMTKSKT
                     AGNYINSVLAKHEVKKSGYDEAIMLDAEGYVSEASGENIFIVRDRKIKTTPLTSILPG
                     ITRDSIIALARDKGYELVEERFTRDELYIADEAFFTGTAAELTPIREVDDRQIGSGRR
                     GPVTEDLQSTFFDIIKGKNSRYEKWLAYV"
     gene            4728..5096
                     /locus_tag="VFY96_16780"
     CDS             4728..5096
                     /locus_tag="VFY96_16780"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX5608183.1"
                     /translation="MSDSPDPTLRVSMLPRDTNARGTIFGGVILSHLDLAGGIAASRV
                     AARNFVTRAMRGVEFIAPVYVGDVVSFYTSIVRRGTTSITVKVSVEAERGRDPRQRVK
                     VTEAEVVYVSVDEAGKPLSL"
     gene            5112..5957
                     /locus_tag="VFY96_16785"
     CDS             5112..5957
                     /locus_tag="VFY96_16785"
                     /inference="COORDINATES: protein motif:HMM:NF045274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5608184.1"
                     /translation="MGLVQKTASVIFLLVLFGGFMATARAADGPGGHSADCCQHENAQ
                     IARWQQNADRLYARFKPREAAAELQKVLEIDADNFAALIKLARAYIDIGDHIAENGSD
                     SKERKMKEYARAEDYARKAVKVDPSSTWGHFWVAASLGNIAMVSPVEKQLDLAGEIRD
                     AVEESIARDPQNGLAYHIYGVWHRKLAELGQGKRLLAGVFYGRSPPSGSLQKSIEYLK
                     KAVALNPTVILCRLELARSYVAVQEFADARAMLQSVPALPIQFSDDAKHKQESAQLLQ
                     QIKDS"
     gene            6002..>6808
                     /locus_tag="VFY96_16790"
     CDS             6002..>6808
                     /locus_tag="VFY96_16790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5608185.1"
                     /translation="MHHQSTFSINVAMRPVWIAVGIGLSLAVFTLACGNNRSNTKALR
                     VGFVPAEDAQQVMQNAQPLVDILRKELGMEIEPFVATDYTGIVEALRVHKLDVAFLAP
                     ASYVLAKNEANIKVILKSERKGIPFYYAAIITRADSGIKTLDDLRGKSFAFGDTLSTT
                     GHVFPRKMLKERGIDPTRDLKQILYSGGHDATVLAVLNGKVDAGATYANSPDSNDTAW
                     MRYLKDPADIKRIRAIAFSEPIPADNLVVSGALDPKIVKRLEEVFIELSRD"
BASE COUNT         1523 a         1876 c         2000 g         1409 t
ORIGIN      
        1 acccaggctt cactcattaa agagctggat gagcgcggca tcggtcgtcc ctccacttat
       61 gccgcgatca tctcgaatat tctcgatcgc gagtacgtta tccagaacga agggcgttcg
      121 ctcgcgccta ccgatttggg ttttttggtg accgatctgc tggtcgaaag ctttccggat
      181 attttgaatg tcgagttcac tgccggcatg gaagatgagc tcgacaaaat cgaggacggc
      241 aaagagaagt ggaccaaggc gatgaagcgt ttttatacgc cgttctcgcg ggatctgaag
      301 aaggccgaaa aacaaatgcg cgacgtcaag cggcaggagg tacccaccga tatcaattgc
      361 gataagtgcg gcgcaaagat ggtgatcaag tggggccgca acggcgaatt cctcgcttgt
      421 ccgcaatatc cagaatgcaa gaacaccaaa aacttcaaac gcgaagacaa cggcggcatt
      481 gaaatcgttg ccgacgaaga agtcaacgaa gcctgcgagc tctgcggccg acccatgctg
      541 ctccgctggg ggaagttcgg caaatttctc ggctgcagcg ggtatcccga atgcaaaaat
      601 atccagccgc tggagaagcc ggtggatttg ggtatcaagt gtccggaatg caaggaaggc
      661 aacatcaagg agcgaaaatc ccgttggggc aagatttttt acggctgcga ccgttatcct
      721 gagtgcaaat tcgcttcgtg ggataagccc atcgcgatcg cctgtcccga ttgcggcagt
      781 ccgatcctcc tggaaaagct gaccaagcga gcgggacgag tgcagcggtg ctacaataaa
      841 gaatgcggct attcagttca ggtggaggca ccgcttagca ccaaaaagcc gccggcggat
      901 cattcttcgg cggcctccgc ataaaattcc atccggtaac ggtcgtgact tccatcccgc
      961 gcgtttctct gtaattgtta attccgtgtc agatcctgtg tgcattatcg gcggcggctt
     1021 agctggcgcc gaagcggcgt ggcaactcgc tcggcgaggt gtggcggtgg agctctatga
     1081 aatgcggccg cagcgctcga ccgatgcgca cgccggaagc gatctgggtg aactggtgtg
     1141 cagcaattcg ctacgctccg atctgctgtc ggcaccggcg gggctactca aagccgagat
     1201 gcgccggctc gactccatca ttgttcgttg tgccgagcag caccgcgtcc ccgcgggttc
     1261 agcattggca gtggaccggg atgcttttag cgctggcttg actgaaatcg ttcaagaatt
     1321 gccgggagtt cggatcattc gcgaagaagt tcgagagatt ccggacgagg gcgtggtcat
     1381 tgttgccacc gggccgctca cgtctccggc gctatcgggt caattggctg cggcgctagg
     1441 cgagaagcac ctgtattttt acgacgccat ttcgcccatc gtgactgccg attcgatcga
     1501 tatggagctt gcattccgag ccgcgcggta cggcaaaggc ggtgacgatt atctgaatct
     1561 gccgctcacg cgcgaggaat attaccgctt cgtcgatggc ttgctggcgg ccgagcgcgt
     1621 gccgacccac gccttcgagc gcttcgtcgc tttcgcgggc tgcatgccga tagaggaaat
     1681 ggccgaccgc ggcaaagata cgctctcctt tggtccgatg cgggcggttg gactggtgga
     1741 tccacgcagc ggcaaacggc cgcatgccgt agtgcagctg cgtcaagaga accgagctcg
     1801 cacgctttac aacctggtgg ggtttcaaac caaaatgacc tattcggagc agcgccgcgt
     1861 ttttgcgctg atcccaggat taggtaaagc agaattcgtc cgtctcggga gcatgcaccg
     1921 taatactttt attaatgctc ctctgcacct cgagccgacc ttgcaatggc gccgccgtcc
     1981 caccttgttt tttgccggcc aaatgaccgg cgtcgaaggc tatatcgaat ccggcgcaac
     2041 tggaatgctc gccggaatca atgccgccag gctgctggct ggcgacgcac cggtcgtgcc
     2101 cccgatcacc acggcgctgg gctcactgct cgcctacatt acggactgcg aacgcaagca
     2161 atttcagccg atgaatgtga atttcggctt gatgccgccg ctggcgaaac ggctgcgtgg
     2221 caaagcaaaa aaggaaatga tgtcccgccg cgcgcttgcc gatctgaccg cttgggcagg
     2281 agaaaccggc cttattccct cgagcacaga aaccgttgcg gtttcatgag cccgcgacgc
     2341 agcgcatttt tcgatatcgg cacgaacacg attctctgct tgatcgcgga aatcagagat
     2401 accggcagat tcagagtctt ggacgatctg gcggaaatcg ttggactcgg ccaaggcgtg
     2461 gatcaaacgc gcttgatttc agcggaagcg gcacgacgcg cctcgaacgt gctgcagcga
     2521 ttcgttcacc aatgtcggtc cttgggggtc gaggagatga cagcagtggg gacgagcgcg
     2581 ctccgcgatg ccaacaacag cgagcaggta cgcagcaggt ttcgcgatga gatcggactg
     2641 gatgtacgcg ttctgtccgg cgccgaggag gcagcgtatt cgtttcttgc cgtccagcgc
     2701 ggtctgccgc tggccaatcg cgagttgctg gtgatcgata tcggcggcgg cagcaccgaa
     2761 tttattcgcg gtaacgactc aggggtattg cgcgcaatca gcatcgatat ggggtcgatg
     2821 cgcctaacgg agcgatttct ccattccgat ccggtctcgc ccgccgaagc ggaaaccatg
     2881 atcactgcca tagaccgaga actcgcggcg ttaccgagtc gtggcatcga cgcaagctcc
     2941 gcggttgcgc tcgtcggcat tgcagcgaca ttcactactt tggcggcgat ggaaaagcgg
     3001 ctcgtaactt actcgcatcc ggagatccac ggcagtcgga tcacagccgg acaggtgcgc
     3061 gcccaagtgt cttcgcttca gatgaagacg atcgctcaac gaaagggact gatcggcctc
     3121 gatccgaagc gcgcggaggt gatctttgca ggcgcatgtc tgatcgacag gatcatgagt
     3181 ttctatcagc aagacgaggt ggtggtcagt gatcagggcg tgcgctacgg attattgcac
     3241 gaagccgcgg aagcaatgcc aaagggttga catttttggg cggattggaa atgatcgatc
     3301 tttcgtttta tttgcgaaca gtaaccgcca acgaagcacc tacgatagta ctagacggag
     3361 gctcgagcca tgacgaaagc agagttagtc gaggccgttg cgaagagcgc gaaaatcagc
     3421 aaagtaaccg ccacagaggc tgtggatgcg acattcgatc acatcggcag agcgatcaga
     3481 aaaagtaaac ggttccaagt cccggggttc ggaaccttca ccgtccgttc gcgcaaggcg
     3541 cgcaagggcc gtaacccgca aaccggcgcg gtcataaaca tcaaggcgag ccgctccgtc
     3601 ggattcaagc ctgctcctgt attgaagaac ggcttgtagc ctgtgacgga tcgcgcgcaa
     3661 gccaccgcgg attcccggtg gctttttttt gttcttattg atgagcaggc taacggtgcg
     3721 tagaatgcct gaagccgaga aagtgaatat gtcgcttaaa actgaatcga tctggatgga
     3781 tggggagttg gttcgctggg aagacgcgaa agtccacgtc ctcacccacg cattgcatta
     3841 cggaaccgca tatttcgagg gcatccgctg ttatctcttg accgacggcc gttcagcagt
     3901 attccggctg gccgagcaca cgcgccggct tgtggactcc ggaaagattc ttggatttcc
     3961 catcccctac accgaagagc agctcaatcg ggctacattg gacgtgattc gagccaacaa
     4021 gctcaaggaa tgttacattc gaccgttggc attcgtcggg cttggcgagt tgggtctttt
     4081 cgcgccaaaa aatcccgtca gtgtgtgtat cgcagttttt ccgtggggtg catacctggg
     4141 cgatgaaggg ctgagaaatg ggattcgggc gaaggtctcg tcgtttaccc gccatcatgt
     4201 caacgtcatg atgaccaaga gcaagacggc aggaaactat atcaactccg tgctggccaa
     4261 gcatgaggtg aaaaaatccg gctacgacga agccatcatg ctcgacgccg aaggctatgt
     4321 gtcagaagcg agcggcgaga acattttcat cgtccgtgat cggaaaatca aaaccacacc
     4381 gctgacctcc attctgcccg ggatcactcg cgattcgatt atcgcgctgg cgcgcgacaa
     4441 aggctatgaa ttagtcgagg agcgatttac ccgggatgag ctctatatcg ccgacgaggc
     4501 ttttttcacc ggcacggcgg ccgagctgac gccgattcgg gaagtggacg atcgtcaaat
     4561 cggcagcggc cggcgcggtc ctgtcaccga ggatctacag agcacgtttt tcgatatcat
     4621 caaaggaaag aacagccgtt acgaaaaatg gctggcctat gtttaggcga taacaccgac
     4681 cttacctcgt cagcgtcgcg ctccaaccgc aggcttcgat tacaaggatg agcgactctc
     4741 ccgatcccac actgcgcgtg agcatgttac cgcgcgatac caatgcccgc ggtacgattt
     4801 tcggcggcgt aattctgagc catctcgact tggcgggagg catcgccgcc tcgcgcgtcg
     4861 cggcacgaaa cttcgtcacg cgggctatgc gcggcgtcga gttcatcgcg ccggtgtatg
     4921 tgggcgacgt ggtcagcttc tatacttcca ttgtgcgccg gggaaccact tcgatcacag
     4981 tcaaagtcag cgtcgaagcc gagcgcggac gcgatccacg tcagcgggtc aaggtcaccg
     5041 aggcggaagt tgtctacgtg tctgtcgatg aggcgggaaa accgctatcg ttataatagt
     5101 gggttaaagc catggggctg gtgcaaaaaa ccgcaagcgt aattttcctg ctggtcctgt
     5161 tcggcggctt tatggcgacg gcgcgcgctg cggatggccc aggaggccac agtgcagact
     5221 gttgccagca cgagaacgcg caaattgccc gttggcagca gaacgccgac cgtctctacg
     5281 cgcgcttcaa accgcgtgag gccgccgccg aattacagaa ggtcctcgaa atcgatgccg
     5341 acaattttgc ggcactgatt aaactggcgc gcgcctacat cgatatcggc gatcacattg
     5401 cggaaaacgg gagcgactcg aaagagcgca agatgaagga gtatgccaga gccgaagatt
     5461 acgcccgcaa agcggttaag gtggatccca gctcgacctg ggggcatttc tgggtggcgg
     5521 cctccctcgg caacattgca atggtatcgc cggttgaaaa gcagctggat ctggccggcg
     5581 agatccgcga tgcggtcgag gagtcgatcg cccgcgatcc acagaatggc ctcgcttacc
     5641 atatttatgg ggtttggcat cgcaaattgg ctgagctggg ccagggcaaa cgcctccttg
     5701 ccggcgtgtt ttacggccgc tctccaccca gcggcagtct gcaaaaatcc atcgagtact
     5761 tgaaaaaagc cgtcgcgctc aatccgacgg tgatcctgtg ccgactcgaa ctggcgcgga
     5821 gttacgtcgc cgttcaagaa ttcgccgatg cccgcgccat gctccagtcc gttccggcac
     5881 tgccgattca attttccgac gatgccaagc acaagcaaga atcggcgcag cttctgcaac
     5941 agatcaaaga cagctgacgc gctcgagcct attggcgcgg atcagctagc accgatagcg
     6001 attgcaccat caaagcacat tcagtatcaa tgtggcgatg cgccccgtgt ggatcgccgt
     6061 cggaatcgga ctctctttag cggtgtttac tctcgcctgc ggcaacaatc gcagcaatac
     6121 aaaagcgctc cgtgtcggct ttgttccggc tgaagacgcc cagcaggtca tgcaaaatgc
     6181 tcaaccgctg gtggacatcc tgcgcaaaga gctgggcatg gaaattgagc cgtttgtggc
     6241 caccgattac accggcatcg tcgaagctct gcgggtgcac aagctcgacg ttgcttttct
     6301 ggcgccagcc tcctatgtac tcgcgaaaaa tgaagcgaat atcaaagtca ttctgaaatc
     6361 tgagcgtaaa gggatcccct tttattatgc cgccattatc acccgagcgg acagcggtat
     6421 taaaactttg gatgatttgc gcggcaagag cttcgcattc ggcgacacgc tgtcgaccac
     6481 cggccacgtt ttcccgcgca agatgttgaa ggagaggggc atcgatccga ctcgcgattt
     6541 gaagcagatt ctttattccg gcggacatga cgcgaccgtg ctcgcggtcc taaacggcaa
     6601 agtcgacgcc ggggcgacct atgccaactc accggacagc aacgacacgg cttggatgcg
     6661 ctatctcaaa gacccggcag acataaaacg cattcgcgcg atcgctttct cggagcccat
     6721 tccggcggac aatttagtcg tgagtggcgc tctcgatccg aagattgtca aaaggcttga
     6781 agaggtgttt atcgagctca gccgcgat
//
LOCUS       DASULZ010000817         4561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_804533, whole genome shotgun sequence.
ACCESSION   DASULZ010000817 DASULZ010000000
VERSION     DASULZ010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804533"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1583
                     /locus_tag="VFY96_16795"
     CDS             <1..1583
                     /locus_tag="VFY96_16795"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX5608186.1"
                     /translation="GAPQAQPRPEDQMRIVADPATNSLVVFGTAQEFQNIKNILRELD
                     AVPRQVLLDVFIAEVTLKEHESLGVDYQILTKDRVTIFGQTFGSRGALRTLGQLFPNT
                     DFTNTAGGGLTGIVGGNTVQALINALKTDSRVKILSSPSVLATDNRPARIQVGSEEPI
                     ATGQLIAATGATTPSSSTSIQYRNTGRIVTIIPQVNSQGLVNLQILAEVSQRGVDVKV
                     GQDTFPAFDTRQAETTAVVQDGDSLVIGGIIADNRARSRTGIPYMMDIPVIGRFFATN
                     SDEILRTELIMLITPHVIYNRDQGQQVTEDFKKSLATVRNELDRIARERQKLQQQPLE
                     QPQTPSLPGPSGDVVPPAPAPSAAPAAPQNPVTPLRQINSLSPVLPGLPQPPGGSVTA
                     EVSRESEKIQSAAPAYALSLTRPVEPARPARPTAPVRPATPPLAAPAPPPFAAPRSTR
                     EWAVQVAALATDKDAQTMAEFLRKSGYDSYVMVFENQGKTWHRVRVGKFADVRSAMQL
                     KNSLTDLAQFKQAYVAGN"
     gene            1865..2785
                     /locus_tag="VFY96_16800"
     CDS             1865..2785
                     /locus_tag="VFY96_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5608187.1"
                     /translation="MNPNQLTIEGVQEILLKEKYVCDRALATVVYLSLVMGKPLLLEG
                     EAGVGKTEIAKVLAKVLDTKLIRLQCYEGLDANTALYEWNYPKQMLRIKLEEVEHGDK
                     QKVETEIFSQDYLIKRPLLEAIQSNGANAPVLLIDEIDRADMEFEAFLLEVLSDFQIT
                     IPELGTIAARHRPFVFLTSNRTREIHDALKRRCLYHWIEYPTFEKEYEIITTKFPEVE
                     TKMAQQICAFMQKVREMNFYKRPGVAETLDWASALIALNRKNLDDKTVVETMGCVFKY
                     REDLHHLRDQVENRQLNLDALLKVSPEMVS"
     gene            2792..4006
                     /locus_tag="VFY96_16805"
     CDS             2792..4006
                     /locus_tag="VFY96_16805"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5608188.1"
                     /translation="MEVTPETVAAAVSSVSSARGKGTLPNVMAFARALKELGVKVSLS
                     QVLDASRSVEHVELAEKFDFRTLLRANMISQKEDFPIFDMLFDCFWRELSYERMPMET
                     MQIEGTPSESQAPEGGDEEGGLEEAAAESLAKENLPLENLEEFSVPTYSPQELMNRKD
                     FSEMGVEESRAIARAILLIATKIATQISRRKKLGRKGNTVDPRWTMRRSMKYGGEIID
                     LVHRKRRIKKTKVVLLCDVSGSMDCYSRFLIQFMYGLQNELWGVETFVFSTSLSRITH
                     LIRTKDIVNALEKISGSILGWSGGTNIGRSLHTFNRNFAPSMVTHRTVVVIISDGWDR
                     GDVSVLEREMQDLKRRCKKIIWLNPLLASENYEPLCKGMQAALPYLDLFLSVHNVNSL
                     VSLGRTLQKMVA"
     gene            4129..4524
                     /locus_tag="VFY96_16810"
     CDS             4129..4524
                     /locus_tag="VFY96_16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX5608189.1"
                     /translation="MAGPGACYTDADSESKKKDSIYHQVKEFLDKGETLAVATIVSTK
                     GSTPREVGAKMVVTAWGEILGTIGGGCGEADVKREAIEVIRTRKPRTVRVDLLDDISS
                     DSPAVCGGIMNVFIDPWWEESDRKAAVEK"
BASE COUNT         1093 a         1186 c         1285 g          997 t
ORIGIN      
        1 tgggcgcgcc ccaagcgcag cccaggcccg aggatcaaat gcgcatcgtt gccgatcccg
       61 cgaccaattc tttggtcgtt ttcggcacgg ctcaagagtt ccaaaatatc aagaacattc
      121 tcagagaatt ggatgccgtt ccgcgccagg tcctgctcga tgtcttcatc gcggaagtca
      181 ccctcaagga acacgaatcg ttaggtgttg attatcaaat tttgaccaaa gacagggtga
      241 cgatattcgg acagacattc ggttccaggg gagctctgcg aactctcggt cagctatttc
      301 ctaatacaga ctttaccaac acggctggtg gcggtctcac cgggattgtg ggcggcaata
      361 cagttcaagc gttgatcaac gcgctaaaaa cggattcacg tgtgaagatt ctctcttcgc
      421 cgagtgtttt ggcaacggac aaccgccctg cccgaatcca ggttggcagt gaggagccga
      481 tagcgacagg tcagctcatt gccgctacag gcgcgacgac accgtcatcc agtacatcga
      541 tccaatatcg gaataccggg cgcattgtaa ctataatccc tcaagtcaat tcacagggac
      601 tggtgaacct gcagattctc gctgaagtta gccagcgagg ggtggatgtc aaagtgggac
      661 aggatacctt tcccgctttc gacacaagac aggccgagac aaccgcggtt gtacaagatg
      721 gcgatagcct tgtcattggc ggaatcatcg ccgataaccg tgctcgttcg aggaccggga
      781 ttccctatat gatggacata ccggtgatcg gccgtttttt cgctacgaac agtgatgaaa
      841 tcctccggac cgagctgatt atgctgatta ctccccacgt aatttataat cgtgatcagg
      901 gtcagcaagt cactgaagac tttaaaaagt cgctcgccac ggtgcgcaat gagcttgatc
      961 gaatcgcccg ggaaagacaa aagctccagc agcaaccatt ggagcagcca caaacaccgt
     1021 ctctgccggg gccgtctggc gatgtcgttc ccccggcgcc tgctccgagc gctgctccag
     1081 cagcaccaca gaacccagtc acgccgttgc gccagatcaa ttcactgtca ccggttcttc
     1141 ctggattgcc ccaacctcca ggagggtcgg tgactgcgga ggtatcacgc gaatccgaga
     1201 aaatacagtc agccgcgccg gcttacgcct tgtcattgac acgcccggtc gaacccgcac
     1261 gacccgcacg cccaaccgcg ccggttcggc cagcgactcc ccccttggcg gctccagccc
     1321 ctccaccgtt cgctgctcct aggtcgacgc gcgagtgggc ggttcaagtc gctgccttgg
     1381 ccaccgacaa ggatgcccaa acgatggctg agtttttgcg caagtctggc tacgactcct
     1441 acgtgatggt ctttgaaaat cagggcaaga cgtggcaccg tgtgcgagtt ggaaaattcg
     1501 cagatgttcg atcggctatg cagttgaaga actcccttac cgacctcgct caattcaagc
     1561 aggcgtacgt cgcgggtaat taggcgctcc gctcccgaaa atagcgcaga aactttgaac
     1621 cggtgatctg gtatccactc ggatcaccgg ttcttcctct ttttggcgat tttcctattc
     1681 aaccttggtt tgttatgatg aattggctgc tgagcggcca ttcattcagc cggtcggacg
     1741 ttgatcgtcg gtaccaaacg gcgtatcgtg attgggtttg ctgttaactc gacgtgtgcg
     1801 gctttgacgt tcaagcaggt ttccgggccc gaagttcaag caattatcat ggggtgaggc
     1861 aagcgtgaat ccaaatcaac tgactattga aggcgtccag gagattcttt taaaagaaaa
     1921 atatgtttgc gatcgcgcat tggcgacggt tgtctatctt tctttggtca tgggcaaacc
     1981 gctgctgctc gaaggagaag ctggtgtcgg caaaaccgaa atcgcgaaag ttctggcgaa
     2041 agttttggac acgaagctga ttcgtctcca atgctacgaa gggctcgacg ccaacacggc
     2101 tctttatgag tggaattatc ccaagcagat gctgcgcatc aagcttgaag aagtcgaaca
     2161 tggcgacaag caaaaagtgg agacggaaat cttcagccaa gactatttga tcaaacgtcc
     2221 gctgctcgag gcgattcaat ccaatggcgc gaacgctccg gtgctcctga tcgatgagat
     2281 cgatcgcgct gatatggagt tcgaagcttt tttgcttgag gtgctgtctg actttcagat
     2341 cacgatcccg gagcttggta cgatcgcggc aaggcatcgt ccgttcgtat ttttgacctc
     2401 caatcgcacg cgggaaattc atgacgcgct caagcggcgc tgtctctacc attggatcga
     2461 atatccgact tttgagaaag aatacgagat catcaccacg aagtttcccg aggtggaaac
     2521 caaaatggcc cagcagattt gcgcattcat gcagaaagtg cgcgagatga atttttacaa
     2581 gcggcccggg gtcgcggaaa ccctcgattg ggcctccgcg ttgatcgcgc tcaatcgcaa
     2641 gaacctggat gataagacag tggtcgaaac catgggatgt gtgttcaaat accgcgagga
     2701 cttgcaccat ttgcgcgacc aggtcgagaa ccgacagctg aacctagacg cgctgctcaa
     2761 ggtatcgccg gaaatggtga gctgattgac catggaagtg acccccgaga ctgttgctgc
     2821 cgcagttagt agcgtctcgt cggcgcgtgg caaaggaacg ttgccgaatg ttatggcgtt
     2881 cgcacgggcg ttgaaggaat taggcgtcaa ggtgagcctg agtcaggtgc tggatgcatc
     2941 gcgctcggtg gaacacgtcg agctcgcgga aaaatttgat tttcgcacgc tgctgcgcgc
     3001 taatatgatc tcgcagaaag aagatttccc gattttcgat atgttgttcg attgtttttg
     3061 gcgcgagctc agctatgagc gcatgccgat ggagacgatg cagatcgaag gcaccccgag
     3121 cgaatcccaa gccccggagg gcggtgacga agaaggcggg ctcgaagaag ctgctgcgga
     3181 gtcgctggcc aaggaaaatc tgccgctgga gaacttggag gaattttccg tcccaaccta
     3241 cagtccgcag gagctgatga atcgcaaaga ctttagcgaa atgggcgtgg aagaaagccg
     3301 cgccatcgcg cgggcgatcc tgctcatcgc caccaaaatt gcgacccaga tcagccggcg
     3361 aaaaaagctc ggacgcaaag gaaacaccgt cgatccgcgc tggaccatgc gccggagcat
     3421 gaaatatggc ggcgaaatca tagacctggt gcatcggaaa aggcgcatta aaaaaaccaa
     3481 ggtcgtgctg ctttgcgatg tcagcggctc gatggactgt tacagccggt ttctgattca
     3541 gttcatgtat gggctgcaaa atgaactatg gggcgtcgag acgtttgttt tcagcacctc
     3601 gctcagccgc attacgcact tgattcggac caaagatatc gtgaacgctt tggagaagat
     3661 ttccgggagc attctgggct ggtcgggggg gaccaatatt ggccgttccc tgcatacgtt
     3721 taaccgcaat ttcgcgccta gcatggtaac ccatcgcacg gtcgtggtga ttatcagcga
     3781 tggctgggac cgcggcgacg tcagcgtgct cgagcgcgag atgcaagacc tcaagcggcg
     3841 ctgcaaaaaa atcatctggc tcaatccttt actggcgagc gaaaactacg aaccgctctg
     3901 caaaggcatg caagcggcgc tgccgtatct ggatttgttt ctctcggtgc acaacgtgaa
     3961 cagtctggtg tccctgggcc ggacattgca gaaaatggtg gcgtgacgaa gaacggatcg
     4021 agaatcgagg attgaaatgg cgagggattt ccctcctgga gtttagcgaa tctcgccgca
     4081 atcttcgatc gtctatgaga aagttgacta cgattcggag gagcgaacat ggctggacct
     4141 ggtgcttgtt acacggatgc agattctgaa agtaaaaaga aggattctat ctatcaccaa
     4201 gtcaaagaat tcctcgacaa gggcgaaacc ttggctgtcg ccacgatcgt gtcgacgaag
     4261 ggatcgacac cgcgcgaagt cggcgccaaa atggtggtca ccgcctgggg cgaaattctc
     4321 ggcaccatcg gcggtggctg tggcgaagcg gatgtgaagc gcgaagcgat tgaagtgatc
     4381 cgaactcgca agccaagaac cgttcgcgtc gatctgttgg acgatatttc ttccgacagc
     4441 ccggcggtct gcggcgggat catgaacgtg tttatcgatc cgtggtggga agagtcggac
     4501 cggaaagctg ccgtcgaaaa atgacctcgt ttgcggaaca attggtcgaa atcaacaacc
     4561 a
//
LOCUS       DASULZ010000818         2948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_397929, whole genome shotgun sequence.
ACCESSION   DASULZ010000818 DASULZ010000000
VERSION     DASULZ010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397929"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..940)
                     /locus_tag="VFY96_16815"
     CDS             complement(236..940)
                     /locus_tag="VFY96_16815"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX5608190.1"
                     /translation="MAESFHDRLVATRDKNHSKDHPFFNLWAAGKLTKEQTAFYCTQH
                     FHYVTEYLNWLAYEASQIAHRDVKAYLLENLGDEEDPEDRHLDMLKDYVAASGAPRDS
                     VERSIVLAGTDQLQNWGWRMVYQSPWQGVVAGLFIGLESQFLDICKKIVPALHRHYGY
                     TPHAREIRFFEEHIHADEVHGAKGFAIVEKYCDTPELQDLAVKQVEEATVRRWRYMNS
                     IYWYALHGKIDDTPPL"
     gene            complement(1033..2769)
                     /locus_tag="VFY96_16820"
     CDS             complement(1033..2769)
                     /locus_tag="VFY96_16820"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5608191.1"
                     /translation="MIETKGLTGAQSMLRVLAKMGVEKIFASPGSEWSPVWEALAEPG
                     VEGMPQYYSTRHEEVAVGMASGYAKSTGKLPAVMIHTTVGSLHATMGMRAAVREQVPM
                     VVLTGESVGFGEDEGPDPGGQWLGHLSNIGGPAKLVESTVKWSFGINNKAILPATIQR
                     ACQLAMAAPKGPTFVSLPMEYLFAKMIKDVPGEHGLAPGATADPKGIEELAKLLAGAK
                     NPVIVAEDAGRTEKSVHCLVEIAELLGCAICETRSTGAVNFPRTHPLHSGYDAKKACS
                     GADVILLLAVIAPWHPASITPAPGATVALLDENPFRHDMPYYGYQADLCLTGEIESSL
                     ESLLAALKKKVSASDPSRKQRAEELGSKNNAQRKAWRDEAIALANQKPMDTRWVAHEL
                     SQVLPSDAMIVDETITHRLALHRYLDGVRAGSWFAGCIGGLGTGLSTALGVKAANPKR
                     PVVCVIGDGSFNYDPVPGVYGAAQEHNLPFLTVMFNNQGYLSQKSGIPRYYPDGWAVK
                     SKNFSGLHIAPCPEYSQIMKAFDGYGEKVEEPGEVRKAIERGLRAVAAGQAALVDIRL
                     KPVEQIVERGEG"
BASE COUNT          623 a          854 c          790 g          681 t
ORIGIN      
        1 gaatctcttc gggttcattc cccgccgcat gctgcgtcga aaggaatcgt caatccgaaa
       61 tccgtcattc ccgcatgttt taagcgggaa tccaggcgag tccgaatctg gacccccgat
      121 agaaacattc gggggtgacg agaagcgaac cgttatatgg gtttggattc cttacctact
      181 gcctaatcgc tttcgtcttt tgtagaagca cgccagaaaa aataatctaa cggcattata
      241 acggcggtgt gtcgtcgatc ttgccgtgca atgcatacca gtaaatgctg ttcatgtagc
      301 gccagcggcg cacggtcgct tcctcgactt gcttgacggc gagatcctgc agctcaggtg
      361 tgtcgcaata tttttcgacg atcgcaaacc cttttgcacc gtgaacttca tccgcgtgga
      421 tatgttcttc aaagaagcga atctcgcgag catgcggtgt gtagccatag tgccgatgca
      481 gcgcgggaac gatttttttg cagatgtcga gaaactgcga ttccaacccg atgaacaatc
      541 cggcgacgac tccctgccat ggcgattggt aaaccatgcg ccagccccaa ttctgtaact
      601 gatcggtgcc ggctaacaca atggatcgct ccaccgagtc gcgcggcgca ccgctcgcgg
      661 cgacataatc tttgagcata tcgagatgac gatcttcggg atcttcttcg tcacctagat
      721 tttccagcag ataggctttg acgtcgcgat gagctatctg cgaggcctcg taggcaagcc
      781 agttcaaata ttccgtgacg taatggaaat gttgcgtgca gtaaaaagca gtctgttctt
      841 tggtcagctt tcccgctgcc cataaattaa agaacgggtg atccttggag tgatttttgt
      901 cgcgcgtcgc gaccaagcgg tcatgaaaac tctcggccat ctaagctctc gcctctatca
      961 tcaaaatagc aacgtcggcg gcgtgtgact acgcgcaccg acgttacaga gttaaacagc
     1021 tcgggagatc tgctagcctt cgcctcgctc aacaatctgc tccaccggct tgagtcgaat
     1081 atcgaccagc gccgcctggc ctgcggccac ggcgcgaaga ccgcgttcga tggccttgcg
     1141 cacctcgccc ggctcttcga ctttttcccc gtaaccgtcg aacgctttca tgatctgtga
     1201 atactccgga caaggtgcga tatgcagacc ggaaaagttc ttcgatttaa ccgcccagcc
     1261 atcgggataa taacgcggaa tgcccgactt ttgagataga tagccctgat tattaaacat
     1321 tacggtgagg aatggcagat tgtgctcctg ggccgcgccg tacacccccg gcaccggatc
     1381 gtagttgaag gagccgtcgc caatcacgca aaccaccggc cgtttaggat tcgcagcctt
     1441 tacacctaag gcggtcgaaa gcccggtgcc caaaccgccg atgcagcccg cgaaccacga
     1501 gcctgccctg actccatcga gataacgatg aagcgccaac cggtgagtta tggtctcgtc
     1561 cacgatcatg gcatccgaag gcaaaacttg actgagttcg tgagcgaccc agcgggtgtc
     1621 catcggcttt tggttggcga gcgcgatcgc ctcgtcgcgc caggcctttc tctgcgcgtt
     1681 gttcttgctg ccgagttcct cagcgcgctg cttgcgcgac ggatcgctcg ccgagacttt
     1741 cttcttgagg gcggccaaca agctctccag ggagctttcg atttcacccg tcaagcaaag
     1801 atccgcctga tagccgtagt acggcatatc atgacggaag ggattttcat cgagaagcgc
     1861 gaccgtggct ccgggtgccg gagtaatcga agccggatgc catggagcga tcacggctag
     1921 caacagaatc acgtcggcgc cggaacaggc tttcttcgcg tcgtaaccgc tgtgcagcgg
     1981 atgtgtgcgc ggaaaattaa ccgcaccggt gctgcgggtt tcacaaattg cgcagcccaa
     2041 gagttcggcg atttcgacca aacaatggac tgatttttct gtgcgtcccg cgtcttcggc
     2101 gacgatcacg ggattcttcg cgcccgcaag cagcttggcg agctcctcga tacctttggg
     2161 atcggccgta gcgccgggcg ccaaaccatg ctctcctgga acatctttga tcattttcgc
     2221 gaacagatat tccataggca gcgaaacaaa ggttggaccc ttgggcgcag ccatggcgag
     2281 ttgacaggcg cgctgtatcg tcgccggcaa gatggctttg ttgttgatgc cgaagctcca
     2341 tttcaccgtc gactcgacca acttggcggg accgccgatg ttcgaaagat ggccgagcca
     2401 ttgcccgccc ggatcgggcc cttcgtcttc accgaacccc acagattcac cggtgagaac
     2461 aaccatcggc acttgctcgc gcaccgcggc gcgcatcccc atcgtggcat ggagcgagcc
     2521 gacggtggtg tgaatcatta cggccggtag cttacccgtt gatttggcgt agccgctcgc
     2581 cattcccacc gctacttctt cgtggcgcgt gctgtaatat tgcggcatac cttccacgcc
     2641 gggctcggcg agcgcttccc agaccggtga ccattcggat cccggcgatg caaagatttt
     2701 ttccacgccc attttggcga ggacgcgcag catcgattgc gcgccggtca atcctttagt
     2761 ctcgatcatg tgtcaacctt tctgttactg agctacaact ccgattgcat agtgttcgat
     2821 cagcggattg cttgtcgtcc aattcgctac gataaattat aaaaccgttt ggcgttgttc
     2881 agcagaatgt ctttttttgc gtcgggcgag agatcctctc gcgcccaaag aatgctgccc
     2941 gattccgg
//
LOCUS       DASULZ010000819         3185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_703902, whole genome shotgun sequence.
ACCESSION   DASULZ010000819 DASULZ010000000
VERSION     DASULZ010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703902"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="VFY96_16825"
     CDS             complement(<1..937)
                     /locus_tag="VFY96_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017301398.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX5608192.1"
                     /translation="MPNADLDLLVIHPGNRTQIYQSLGSSLSAIEPPVWAGLMATFVR
                     KRGFSVQVLDAEADELTPEQTAIRVAEMNPLLTAVVVYGHQPSASTQNMTAAGAICTA
                     IKQANPERKVLLVGGHAAALPERTLHEEDADFVCYGEGPYTIVDLLQALKFSGHADYA
                     GVRGLCFWADGVLSRTAPAPLVRALDEEMPEMAWDLLPMKKYRAHNWHCFGGLEREPY
                     GSFYTTFGCPYRCSFCCIQAPFKSGEKEAGWKESVNSYRFWSAAAVVAQIDKLVTNYG
                     VRNIKIADEMFVLNARLVQGICDAIIDRGYDLNIWA"
     gene            complement(930..2105)
                     /locus_tag="VFY96_16830"
     CDS             complement(930..2105)
                     /locus_tag="VFY96_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5608193.1"
                     /translation="MRNNIQQTDVDALINFLKPEVPILTQASQVEAFEQEWSRWLGVE
                     HSVFVNSGSSANLLTITALKETYGTGEIIVPTLTWVSDIASVIQCGFKPVFVDIDRRT
                     LGMDTVQVLKKITPRTKAVFLTHVLGYNALDANLLVELKNRGVALIEDVCESYGATFQ
                     GQKLGTFGLMSNFSFYYAHHMSTIEGGMICTNEPQFYETVRMLRSHGMVREAKSGDLR
                     NSYSSRHADLNPDFIFAFPGYNVRSTEINAVIGRSQLKRLDHNNQLRTQNLRLFLSHL
                     DSDKYQTDFALEGSCNYAFTLVLKKANPVFCERVMSRLRELRVEFRRGTSGGGNQLRQ
                     PYAKKLLGATEFEKYPAVNHVHFYGFYIGNYPGLDREKILKLCSALNALPDREGRHA"
     gene            complement(2144..>3185)
                     /locus_tag="VFY96_16835"
     CDS             complement(2144..>3185)
                     /locus_tag="VFY96_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5608194.1"
                     /translation="RELSYVDAVREATDQEMARDSSVILFGLDVDDPKAIQGTTRGLL
                     EKFGADRVFGTPLSEDAMTGVAIGAALAGLRPIHVHIRMDFLMLAMNQLVNIAAKSRY
                     MYGGQVQVPLVVRSMIGKSWGQGAQHSQGLYSFFMHVPGLKVVAPTTPYDAKGCLVHA
                     IRDNDPVMYVEHRILHFQRGPVPESSYTVTPGKARITATGSDVTLVGISYMQIECLHA
                     RKYLEAVGIDAEVIDPIWLSPLDIDTIVESVRKTGRLCVVDNGWVTCGASAEIVALVI
                     EGLQQIRPVVVRRLGFAPVTCPPTPILENLYYPNATTIASAVYDLVRGGDNGWMPEQR
                     LDLQEIQFRGPF"
BASE COUNT          744 a          914 c          823 g          704 t
ORIGIN      
        1 aggcccaaat gtttagatcg taaccgcggt caataatcgc atcgcagatg ccctgcacga
       61 gccgcgcatt caaaacgaac atctcgtcgg cgatcttgat gtttctgacg ccataattcg
      121 ttaccagttt gtcgatctgt gcaacgacgg cagcggcact ccagaatcga taactgttga
      181 cgctctcctt ccagcccgcc tctttttcac cgcttttaaa gggcgcttgg atacagcaaa
      241 aactgcaacg atagggacaa ccaaaggtcg tgtagaatga tccgtacggt tcgcgctcga
      301 gaccgccgaa gcagtgccaa ttatgcgccc gatatttttt catcggcagc aggtcccagg
      361 ccatttccgg catttcctca tcgagcgctc gcactaacgg tgctggcgcg gtcctggaga
      421 gcacaccatc agcccaaaag cagagacccc gcacgcctgc gtaatccgcg tggccggaaa
      481 atttcaaagc ctgcaacagg tcgacgattg tataaggacc ctcgccgtag cagacaaaat
      541 ccgcatcttc ctcatgcaat gtgcgctccg gcagcgcggc cgcatgaccg ccgaccaata
      601 acaccttgcg ttcgggattc gcctgtttaa tggctgtaca aatcgcaccg gcagcggtca
      661 tattttgcgt cgatgctgaa ggctggtggc cgtagaccac cacggctgtc agtaatggat
      721 tcatctccgc gacccgtatg gcggtctgtt ccggcgtgag ctcgtccgct tcggcgtcga
      781 gcacctgcac ggaaaacccg cgtttgcgga caaacgtcgc catcagaccc gcccaaaccg
      841 ggggctcgat cgctgataag gatgagccga gggattgata gatctgcgtt cgatttcccg
      901 gatggatgac caacaggtcc aggtcagcgt taggcatggc gcccttccct gtccgggagc
      961 gcattcaacg cactgcacag cttcaagatt ttttctctat ccaggcccgg ataattgcca
     1021 atatagaagc cgtaaaaatg cacatggttg actgccggat atttttcgaa ttccgtagcg
     1081 cccaagagtt tcttcgcata gggttggcgg agctgattgc cgccgccgga tgtcccgcgg
     1141 cgaaactcga cacgaagctc gcgcagcctg gacatgaccc gttcacaaaa aactggattg
     1201 gcttttttga gcactaaggt aaacgcataa ttacagcttc cttcgagcgc gaaatcagtc
     1261 tgatatttgt ctgaatcgag gtggctgaga aacaagcgca ggttttgcgt cctgagctga
     1321 ttgttgtgat caagccgctt taactgcgaa cggccgatca cggcattgat ctcggtgctt
     1381 cgaacgttat agccgggaaa tgcaaagata aaatccggat tcaaatccgc atgtcggctg
     1441 gaataagagt ttctcagatc ccctgacttc gcctcgcgca ccatcccgtg cgatcgaagc
     1501 atgcgcacag tttcatagaa ctggggttcg ttggtgcaga tcatgccgcc ttcaatagtg
     1561 ctcatgtggt gcgcatagta aaaggaaaag ttcgacataa ggccgaacgt ccctaatttt
     1621 tgcccttgaa acgtagcgcc gtaagactcg cagacatcct cgatcagggc tacgccgcga
     1681 tttttcagct ccacgagaag attagcatcg agagcgttgt agccgagcac atgggtcaaa
     1741 aaaaccgcct tggttcgcgg cgtgattttc ttcagcacct gcactgtgtc catccctagc
     1801 gtgcggcgat cgatgtccac aaagaccggc ttgaatccgc attgaatcac cgatgcgata
     1861 tccgacaccc acgtgagcgt cgggacaatg atttcccccg ttccgtaggt ttctttgagt
     1921 gccgtaatcg tcaaaaggtt tgccgaggag ccggaattga caaaaacgct gtgttcgacg
     1981 ccgagccagc gcgaccactc ttgctcgaag gcttccactt ggcttgcttg agtaaggatc
     2041 ggcacctccg gttttaaaaa gttgatcagc gcatcgacat ccgtttgctg gatgttgttt
     2101 cgcatcagcg gccaatttaa ctcttctcgg cagctcatta gcgctagaaa ggtccgcgaa
     2161 actggatctc ctgaagatcg aggcgctgtt cgggcatcca gccgttatcg cctcctcgga
     2221 cgagatcata gaccgcggaa gcaatggtgg tcgcgtttgg ataatagaga ttctccagga
     2281 taggagtcgg tggacaagtc accggagcga accccaaccg acgcaccacc accggccgga
     2341 tctgttgtaa accttcgata accagcgcaa cgatttcagc gctcgctccg caggtgaccc
     2401 agccattatc gacaacacac agccggcctg ttttgcgcac cgattcgaca atcgtgtcga
     2461 tatccaaggg actcaaccag atcggatcga tgacctcggc atcgataccg accgcttcca
     2521 gatacttgcg cgcatgcaga cactcgatct gcatgtagga aattccgacg agtgtcacgt
     2581 cggagccagt tgcggtaatg cgtgccttgc caggggtcac tgtatacgag gattccggca
     2641 ccggaccgcg ttgaaaatgc aaaatgcgat gctccacata catcaccgga tcgttgtcac
     2701 ggatcgcgtg aaccaaacag cctttcgcgt catacggcgt ggtcggcgcc accactttta
     2761 atcctggcac gtgcatgaaa aacgaataca atccctgcga gtgctgcgcc ccctgtcccc
     2821 agctcttgcc gatcatggac cgcaccacaa ggggcacctg cacttgcccg ccatacatat
     2881 agcgactctt ggctgcgatg ttgacgagtt ggttcatcgc cagcatcaga aaatccatgc
     2941 gaatatggac gtgaatcggg cgcaagccgg ccagagccgc gccaatggcc actccggtca
     3001 tagcatcttc tgaaagcggc gttccgaaaa ctcggtcggc gccaaatttt tccaacaatc
     3061 cgcgtgtggt accctgaatc gctttcggat catcgacgtc caaaccaaac agaatgaccg
     3121 atgaatcccg cgccatttcc tgatcggttg cttcgcggac agcatcgacg tacgacagct
     3181 cgcgt
//
LOCUS       DASULZ010000820         3557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_398056, whole genome shotgun sequence.
ACCESSION   DASULZ010000820 DASULZ010000000
VERSION     DASULZ010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398056"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1857
                     /locus_tag="VFY96_16840"
     CDS             <1..1857
                     /locus_tag="VFY96_16840"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HEX5608195.1"
                     /translation="IMPKGLSLESELRDLSAKLPVDHGLALRTVTGQIRLANRVLAFQ
                     SFKGVYGDSRFTGLDGTYDLSPASGGKLEVYTRADVNLGELKNQLTAQELSPELGRFL
                     ASLEELGGRSRLELSLKRASAQPLQFEGKMSLDNVRARYSEYALNDVQGDLTFNSKEL
                     STEKIRAQLNGSPIQAQIALTDYNTDGASFDLNVESNGMRAGVLTSLLLDTGSPRDTG
                     LVRVALRYTGSFAKNAQRRLTGTLDLLNVQMQLKPLLQPLRALNGRISIDESGIDFQK
                     LNASLVGFPVSATGRWRYRQNPQLVFDFAAPNLDITTLISQIDPELSDFYATLVADGK
                     IALNKGRIKNFEFSDLTTRAAIDHRVWRLSNLRARSGGGEIQGVTTIFDKPDTLAVVA
                     DPKIDGVPIQSFLNWFNVSTTEMTGKVSLNGKLETVGKNDTERKRNLNGAFNLRIEDG
                     TINRMRILVQLLNLLDLSRWFTFQMPDLTKQGIRFRAITGDFKVSNGIYFTDNLIVDS
                     SDLRMTGAGKIDVPKDELDFVVAVRPFAGIDTAMSYIPLLGRSIAAIKNSFLVASFNI
                     TGRIDEPTITPAPLGTLSEWFWGVLGIPKNIIGFGENEKKEPTQPAPLPPLQ"
     gene            1857..2138
                     /locus_tag="VFY96_16845"
     CDS             1857..2138
                     /locus_tag="VFY96_16845"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX5608196.1"
                     /translation="MPKLTLYSRQDCCLCAEMKAVIDQVAGKIPVEIDEIDIDQSPEL
                     RTKFAQEIPLLCIDGRKAFKFRVTPKQLETQLRRHSRRRGYLRWFTERS"
     gene            2209..2571
                     /locus_tag="VFY96_16850"
     CDS             2209..2571
                     /locus_tag="VFY96_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5608197.1"
                     /translation="MANQVVWCDIPVRDLERAIRFYSAVLGLDVKRHDFSGVTVGILP
                     HDEGEVGGCLLTNQEEQPGKDGVMIYLNASGRLDEAVAAVPVNGGSVIKPKHAIGPFG
                     FRAIVIDSEGNRVALHSE"
     gene            2589..2987
                     /locus_tag="VFY96_16855"
     CDS             2589..2987
                     /locus_tag="VFY96_16855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608198.1"
                     /translation="MRYPISQDGFRLIMMLMLIGTPVFLEAAQAGKSKPQKAPAVKED
                     PGPWLLAGREGECVPLSILSKKWPEYTDIQSPHQLAEKLRAAGYDAEIKEFNAGTRPA
                     VEVRAPSAGLDVMFIKKDLCDKPPPTAENR"
     gene            complement(2991..>3557)
                     /locus_tag="VFY96_16860"
     CDS             complement(2991..>3557)
                     /locus_tag="VFY96_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX5608199.1"
                     /translation="LAVRKGKMTKMVHAGSSRVRLEFNVPSRGLIGFRSRFLTDTRGT
                     GIMNALFDGWAPWHGAIQSRSNGAMVSDREGQATPYAVFHLQERGVIFIPPGSRVYEG
                     MVVGEYSRDVDLNVNICREKKLTNIRAAGRDENIIITPHREMGLEEGIEWIADDELVE
                     VTPHSVRLRKKLLRQADRPRRRQEELEN"
BASE COUNT          825 a          983 c         1011 g          738 t
ORIGIN      
        1 atcatgccaa agggactttc gctcgagagc gaactgcgcg acctttcggc caaactaccg
       61 gtcgatcatg gcctcgcgtt gcgcacggtg acgggacaga tccgtcttgc caaccgcgta
      121 ctcgctttcc agagcttcaa aggtgtttac ggcgacagcc ggttcactgg cttggacgga
      181 acttatgatc tatcgcccgc cagcggaggc aagctggagg tttatacgcg cgccgacgtc
      241 aatctcggcg aattgaaaaa tcagctaacc gcgcaggaac tctccccaga attgggccgg
      301 tttttagcat ctttggaaga gcttggcggc cgatcgagac tcgagctcag tctcaaacgg
      361 gcaagcgctc agcctttgca gttcgaaggg aaaatgagcc ttgacaatgt ccgcgcgcgc
      421 tacagcgagt atgccttgaa cgatgtgcaa ggggatctca ctttcaactc gaaagagctc
      481 agtacggaaa aaattcgcgc gcaactcaac ggctcgccga tccaagctca aatcgcactc
      541 acggattaca acactgatgg cgctagcttt gatctcaatg tcgaatcgaa cggcatgcgc
      601 gccggagtgc tgacgagcct cttgctcgat acgggcagcc cgcgggatac cggacttgtg
      661 cgcgtcgcgc tacgctacac gggctcgttc gcaaaaaacg cgcagcgaag attgaccgga
      721 actctggatc tgctcaacgt gcagatgcaa ctcaagcctc tgcttcagcc cctgcgggca
      781 ctgaacggca ggatcagcat agacgagagc ggcatcgatt ttcaaaaact gaacgcctct
      841 ctggtgggtt ttccggtgag cgccaccggg cgctggcgct accggcaaaa cccgcagcta
      901 gtgtttgatt tcgcggcgcc gaatctcgac atcaccaccc tgatttcgca gatcgatccc
      961 gagttgagcg atttttacgc gacccttgtg gcggacggga aaatcgcgct caacaaaggc
     1021 cggatcaaga attttgagtt cagcgatctg accacgagag cagcgatcga tcatcgagtc
     1081 tggcggctca gcaatctcag ggcacgctcc ggaggaggtg aaattcaggg ggtgaccacc
     1141 attttcgaca agcccgatac gctcgcggtg gttgccgatc cgaagatcga cggtgtgccg
     1201 atccagtcgt ttcttaactg gttcaatgtc agcaccactg aaatgaccgg taaagtgagc
     1261 ctcaacggga agctggaaac cgttggaaag aacgacaccg aacgcaaacg aaatttaaat
     1321 ggcgcgttca acctgagaat cgaagatgga acgatcaacc ggatgcgcat tctggtgcaa
     1381 ttgctgaatc ttctcgatct ttcccgctgg ttcacgttcc agatgccgga cctcaccaaa
     1441 caagggatcc ggtttcgcgc gattaccggc gacttcaagg tcagcaacgg catctatttc
     1501 acggataacc tgattgtcga tagcagcgac ctgcggatga ccggcgccgg caaaatcgat
     1561 gtgcccaagg acgagctcga tttcgttgtc gcggtacgcc cattcgcggg cattgatacg
     1621 gcgatgagtt acattccgct gctcgggcgt agcatcgctg cgatcaagaa ctcgttcctg
     1681 gtggcgagtt tcaatattac cggtcgtatc gacgaaccga cgattacgcc tgcacccctg
     1741 ggaacattgt cggaatggtt ctggggggtg ttgggaattc cgaaaaatat tatcggcttt
     1801 ggcgaaaacg aaaaaaaaga accgacccaa ccggctccat taccaccgct tcaatgatgc
     1861 ccaaattgac gctgtattcg cgccaggact gttgcctgtg cgcggagatg aaggcggtga
     1921 ttgatcaagt tgccggaaag attcccgtcg aaattgacga aatcgatatt gaccaatcgc
     1981 cggagctgcg cacaaaattc gcccaagaga tcccgctgct atgcatcgac ggccggaaag
     2041 cattcaagtt tcgtgtaacc ccgaaacagc tggagacgca actgcgccgc cattcgcggc
     2101 gccgcggtta tctacgttgg tttacggaac ggtcgtgaag cggataggta gcggccgacc
     2161 ttgagtgtat ggtttttttt aattatcgtt ttaaggaagg agagcaagat ggcgaatcag
     2221 gtggtgtggt gcgacattcc ggtgcgcgat ttggagcgcg cgataagatt ttattccgcc
     2281 gtcctcggtc tcgacgttaa acgacatgat ttttccggcg tgaccgtcgg tatcttgccg
     2341 cacgacgaag gcgaagtcgg gggctgccta ttgacgaacc aggaagaaca acccggcaaa
     2401 gacggagtga tgatttattt gaacgccagc ggccgcctcg acgaagccgt cgccgcagtg
     2461 ccagtgaatg gtggcagcgt catcaaaccg aagcacgcca tcggcccgtt cgggtttcgc
     2521 gcgatcgtca tcgacagcga aggcaatcgc gtcgcgctcc attcggaatg agtcaggcac
     2581 gaataaacat gcgctatccg atcagtcaag atggtttccg gctaatcatg atgctgatgc
     2641 tgatcggcac tcctgtcttc ctcgaggccg cacaagcggg aaagagcaag ccgcagaaag
     2701 cacccgcggt caaggaagac ccgggtccgt ggttgcttgc tggcagagaa ggcgaatgtg
     2761 tgccgctatc gatacttagc aagaagtggc ccgaatacac cgatattcag agcccgcatc
     2821 agttggcgga gaagctacgc gccgcgggat acgacgcgga aataaaggag ttcaacgcag
     2881 gcactcgccc ggcagtggag gtgcgcgcgc cgtcggctgg gctcgacgtg atgtttatca
     2941 aaaaggatct ctgcgacaaa ccaccgccta cggccgaaaa cagataatcg tcaattctcc
     3001 agctcttctt gccggcgtct cggccggtcg gcttgccgaa gaagtttttt tctaagtcgg
     3061 acggagtgcg gtgtgacttc caccaattcg tcatcggcga tccattcgat cccttcttcg
     3121 agccccatct cacggtgcgg tgttataatg atgttctcat cgcggcccgc ggcgcgaata
     3181 ttggtcaact tcttctcacg gcagatgttt acgttcaaat cgacgtcccg ggaatactcg
     3241 cccaccacca tgccttcata gacccgcgag ccgggcggta taaaaatgac gccgcgctct
     3301 tgcaggtgaa aaaccgcgta cggagtcgct tgaccttctc gatccgatac catggcgccg
     3361 ttgctgcggc tttgaatcgc gccgtgccac ggcgcccaac cgtcgaaaag cgcgttcatg
     3421 atgccggtgc cgcgcgtgtc ggtgagaaag cgcgagcgaa aaccgatgag accgcgcgag
     3481 ggaacattga attccaggcg cacgcggctc gagccggcgt gaaccatctt ggtcattttg
     3541 cccttgcgca cggccag
//
LOCUS       DASULZ010000821         6462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_907695, whole genome shotgun sequence.
ACCESSION   DASULZ010000821 DASULZ010000000
VERSION     DASULZ010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6462
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907695"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="VFY96_16865"
     CDS             <1..821
                     /locus_tag="VFY96_16865"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5608200.1"
                     /translation="EEGIDLNVVVMASTPGLQAVLAGEMDFTGSGSSALVAVAQSNAP
                     LKTVLAVNDQVLQWLMVRPQYSSFKDLKNKKVAVTGVAAVATFMLKKVAAKYGLDPNK
                     DLTFLALPPGQRLAGLQSGVVDAGLLSSEERFAALDQGMKEILYLGKEVKNSWGTIAT
                     NDQFIKNKPKLMHGFMRGLLKALRLVKQNREVAMGAMMKFSELDRELAARTYDGMIGT
                     FTSNGIVDEETQKNDLDIVREVLKITKDVPIERAYDFSFAKKADAELTQAGWKP"
     gene            981..1796
                     /locus_tag="VFY96_16870"
     CDS             981..1796
                     /locus_tag="VFY96_16870"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5608201.1"
                     /translation="MAQHVDGPLYYERMGRTGPVIAFIHPNPMDQSCWIFQMAHFSTW
                     YRCIAIDIPGYGRSPKAEAGLTMNDMAEACWEAIDDALPREEAILVGCSVGSSLAPHM
                     YHLRPDKTAALVLCGTGYNPSKEFTKGRIANYTANGIDYRWPYTFEDLSAAFRTTPLA
                     HFFANLFTERNGHADVQTIIHQFKALSQPYPDGLFERIACPTIILTGSEDNSHQRAFA
                     LQERIPNCELKTLPGAGHACQIEQPWLFDRFMIEFLTRHGLFPEGSKATRTII"
     gene            1951..3483
                     /locus_tag="VFY96_16875"
     CDS             1951..3483
                     /locus_tag="VFY96_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEX5608202.1"
                     /translation="MEIEETRDGGFETSNGHVSDRPAEFAFDNTYARDLEGCYVNWKA
                     ARVRSPRLVKFNRELAEELGVDGDALASDRGARIFAGNETPEGAMPLAQAYAGHQFGG
                     FVPQLGDGRALLLGEIIDRHGQRRDIQLKGSGPTPFSRAGDGRAALGPVLREYLIGEA
                     MHSLGIPTTRALAAVVTGEPVFRESVLPGAVLTRVAASHIRVGTFQFFAARGEQDKVR
                     RLADYVIDRHYSEIKGREDPYLELIDRVAERQAALVARWMLVGFIHGVMNTDNMAISG
                     ETIDYGPCAFMDHYDPATVFSSIDSHGRYAYANQPRIAQWNLARLADALLPLIDADRT
                     RAIARATEVVNRFPEKYQQHWLTGMRAKLGLLSEEEADVHLAAGLLTAMEGNKVDYTL
                     AFRFLAEAVIGREERIRVLFADPSIYDLWSEQWRARLSRETTAPQERAAKMRLANPAF
                     IPRNHRVEEALSAAVEQGDYGPFDTLLEILSRPFEDQSEFEAFAQPAPAGQGCYRTFC
                     GT"
     gene            3665..4399
                     /locus_tag="VFY96_16880"
     CDS             3665..4399
                     /locus_tag="VFY96_16880"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009850988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HEX5608203.1"
                     /translation="MPQKFYVVWSGRENGVFSDWATVQRAVDKYPGARFKSFASRAEA
                     ERAFAQGNLEEKSAIPRRSDRRVSHTAHRFDVSIYCDGACDPNPGNAGSGIVVYRGGK
                     LTELWYGLYNPAGTNNTAELNALYHALCMAEAEIKIGKSVEVCSDSAYAINCVRTWAP
                     LWEKNGWKKPGGEIKNLEIIQDCYAIYRRIKDALTLTHVAGHVGTEGNELADRMAMYA
                     AQTREKKLRRYQETMDIPTLLKMRAG"
     gene            4451..4996
                     /locus_tag="VFY96_16885"
     CDS             4451..4996
                     /locus_tag="VFY96_16885"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5608204.1"
                     /translation="MMESAGLVLDALGRVRDMVRDALTDLSPEQLLAPPKPHIAWLVW
                     HIARVQDANFSNLMEHPQLWIADGWHDRFNMAPDPRDYGSGHRHTPAQVDAFAVTETQ
                     LLMDYLDAVFARTKGYLANLTNEDLNRVLNEPQYQPLPTLSIRLASVINCNTRHAGQI
                     EYLRGLAKYGGWFPGAEKMPA"
     gene            5591..6208
                     /locus_tag="VFY96_16890"
     CDS             5591..6208
                     /locus_tag="VFY96_16890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608205.1"
                     /translation="MAVPAVEVVLESAEVPAAELAAALPVQTAVVRGLRTAVLGEPSV
                     VALAAGPVLLRLAALQVPPVVVDQALQVDREVFPVVRVLAPARDPAVIQLGGLPVAPV
                     VVQASPADRQEAPEVPVSAPPAALRVVSPAARAGARLEVRMAAVGLQRVRPAARAMVE
                     AVAARVTGQGAADPVVDRAAGRAIAARLVARPAAAPGLAATDRLV"
     gene            6274..>6462
                     /locus_tag="VFY96_16895"
     CDS             6274..>6462
                     /locus_tag="VFY96_16895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608206.1"
                     /translation="MESSTAGASGTDDKQTGTGSGAADGSASGTIYTFGFWLALLFAA
                     LSSLLVLIWVVVKVVGKNE"
BASE COUNT         1391 a         1797 c         1919 g         1355 t
ORIGIN      
        1 aggaagaagg gatcgatctc aacgttgttg ttatggcttc gacacctggt ctgcaggcgg
       61 tgctcgccgg agaaatggat tttaccggct ccggaagcag cgccctcgtg gccgtcgcgc
      121 aaagcaatgc gccgctcaag acggttctcg ccgtcaacga tcaggtcctc caatggctca
      181 tggtccggcc acagtacagc tcgttcaaag atttaaagaa caaaaaagtt gccgtcaccg
      241 gcgtcgctgc ggtggcgact tttatgttga aaaaagtcgc cgccaagtac ggcctcgacc
      301 ccaacaagga tctgacattt ctcgctctgc cgccgggcca acgcctggcc gggctgcaat
      361 ccggcgtcgt agatgccggt ctgttgagct ccgaggagcg ctttgccgca ctggaccaag
      421 gcatgaaaga gatcttgtat ctcggcaagg aagtgaaaaa ctcctggggc acgatcgcca
      481 ccaacgatca attcatcaaa aacaagccga aactgatgca tggatttatg cgcgggttgc
      541 tgaaggcgct ccgtctcgtc aaacaaaacc gcgaggtcgc catgggcgcc atgatgaaat
      601 tttccgagct cgacagagaa ttggcggcgc gaacctacga cggcatgatc ggcactttca
      661 cgagcaatgg cattgtcgac gaagagacgc agaaaaacga tctggacatc gtccgcgagg
      721 ttctcaaaat caccaaagac gttcccatcg agcgcgctta tgatttcagt tttgcaaaaa
      781 aagcggatgc tgaactgacg caagccgggt ggaagcccta atgtttttcc actcccgggg
      841 aacgtgagca gagtctagtg tgctgtgatt tcgagcgcag tgagaaattt ttcttgatga
      901 tgagcgatca gcgaagaagt ttgctctcac gctaagatcc tcgcttagca tcgcacaact
      961 aaatcaaacg gaggaaaaaa atggcgcagc acgttgacgg acctctttat tacgaacgca
     1021 tgggacgcac ggggccggtg attgctttta ttcatccgaa tcccatggac caatcctgct
     1081 ggatttttca aatggcccat ttctcgacat ggtaccgctg tattgccatc gacatacctg
     1141 gatacgggcg ttcgcccaaa gccgaagctg gactcacgat gaacgatatg gccgaagcgt
     1201 gctgggaagc gatcgacgat gcactgccac gtgaggaagc gattctggtc ggctgttcgg
     1261 tcggctcgtc actcgctcct cacatgtacc atcttcgccc cgataaaacc gcggcgctgg
     1321 ttttatgcgg aactggatac aatccgagca aggaattcac caaaggaaga attgccaact
     1381 acactgccaa tggcattgat taccgctggc cttacacctt cgaagacctg agcgcggcct
     1441 ttcgcacgac tccgctggcg cactttttcg cgaacctttt caccgaacgc aatggacacg
     1501 ccgacgtcca aaccatcatc catcaattca aagcgctgtc acagccctac ccggatgggc
     1561 ttttcgagcg catcgcctgt ccgacgatta ttttgaccgg cagcgaggat aatagccatc
     1621 aacgcgcctt cgcgctccaa gagcgcatac cgaactgcga attgaaaaca ttgccgggtg
     1681 cgggccacgc ttgccaaatc gagcaaccct ggctgttcga tcgctttatg atcgaatttc
     1741 tgaccaggca tggcctcttt cccgaaggtt caaaggcgac gcgaaccatc atttagatgc
     1801 tgctccgaga ttgccggctc tgctgagcag gtcggaagcg attgtccggc gtaggaactg
     1861 ttccgcacgc tgccctgcat tccgttatcg cgaattgctg atctggcagc gcgactttct
     1921 gagtttgttc cggacaggta tagtggtgac atggagattg aggaaactag agacggtggc
     1981 ttcgagacca gcaacgggca cgtcagcgac cgacctgccg agtttgcatt cgacaacacc
     2041 tacgcgcgcg atctcgaggg ttgttacgtc aactggaaag cggcgcgagt ccgaagcccc
     2101 aggttggtga agttcaatcg cgagctggcc gaagaattgg gggtcgatgg cgatgcgcta
     2161 gcttcggatc ggggcgcgag aatctttgct gggaatgaga cgcccgaggg agcgatgccc
     2221 ctggcacagg cgtacgccgg gcaccaattc ggcgggtttg taccgcaact cggcgatgga
     2281 cgagcgctcc tcttaggcga gattattgat cgtcacggac aacgtcgcga cattcagctc
     2341 aaaggctccg gccccacgcc gttttcacgc gccggcgatg gacgggccgc actcggtcca
     2401 gtcctacgtg aatatctgat cggtgaagcg atgcacagcc tcggtattcc gacaacccgg
     2461 gcccttgccg cagtggtgac cggggagccg gtttttcgcg agagcgtctt accgggcgcg
     2521 gtcttaaccc gcgtcgccgc cagtcatatt cgtgtcggca ctttccagtt ttttgccgcc
     2581 cgcggtgagc aggacaaagt ccgccgcctg gccgattacg tcatcgatcg acattattcg
     2641 gaaataaaag gtcgtgagga tccctacctg gaactgattg atcgtgtagc cgagcgacaa
     2701 gccgcgcttg tcgcccgctg gatgctcgtc ggcttcattc atggcgtgat gaacaccgac
     2761 aatatggcga tctccggcga gaccattgat tatggtccat gtgcgttcat ggaccattac
     2821 gatcccgcca ctgtattcag ctccatcgac agccatgggc gctatgccta tgccaatcag
     2881 ccgcgtatcg cgcagtggaa tctcgcgcgc cttgcggacg ccttgctgcc gctgatcgac
     2941 gcggatagga cacgcgccat cgcgcgcgcc accgaggtcg tcaatcgttt tccagaaaag
     3001 tatcaacagc attggctaac gggaatgcgc gccaagctcg ggctcctgag cgaagaggag
     3061 gccgatgtcc atctcgccgc gggattgctc accgcaatgg aaggcaacaa agttgattac
     3121 acactggcct ttcgcttcct cgccgaggct gtgattggtc gggaagaaag aattcgcgtg
     3181 ttatttgccg atccgtccat ctacgatctc tggagcgaac agtggcgagc gcggctttct
     3241 cgagagacca cagcaccgca ggagcgcgcg gcaaaaatgc ggctggccaa ccccgctttt
     3301 attccgcgta accatcgtgt cgaggaagct ttgagtgccg cagtggaaca aggcgattac
     3361 gggcccttcg atactctgct ggagatcctg tcgcgcccgt tcgaggatca atcggagttt
     3421 gaggccttcg cgcaaccggc accggccgga caaggctgct accgcacgtt ttgcggcacc
     3481 tgagtgcggt gcgccttagg gaagaaccgg gagttcgcat tcctctatgc tcttggcggg
     3541 tgcgctctgc gcacctttta ccgcggcaac agcgaaaaca ataaacgacg tttgtatcag
     3601 taccgcggtg gcacaaatat aaaccttcgg tttgcgagct ccgttcgcct gcggtgaatt
     3661 acatatgccc cagaagttct acgtagtgtg gtcaggccgg gaaaatggag tatttagcga
     3721 ttgggcgacg gttcagcggg ccgtcgacaa atatcccggc gcacgtttca agtccttcgc
     3781 atcccgcgcg gaagcggagc gagcgttcgc ccagggcaat ctcgaagaga aaagtgcgat
     3841 cccacggcgc agcgaccgcc gcgtctccca taccgcgcat cgcttcgacg tcagcatata
     3901 ctgcgatggc gcgtgtgacc ccaatcccgg caatgccggt tccggcattg tcgtctatcg
     3961 cggcggcaag ctcacagaac tgtggtacgg cctgtacaat cctgccggga ccaacaacac
     4021 cgccgagctg aacgcgcttt atcatgcatt gtgcatggct gaagcggaaa tcaaaattgg
     4081 caagagcgtc gaagtgtgca gcgactcggc ctacgccatc aactgcgttc gcacctgggc
     4141 gccgctctgg gaaaagaatg gttggaaaaa accgggcggc gagatcaaga acttggaaat
     4201 tatccaggac tgttacgcga tttaccggcg catcaaagac gcgcttaccc tgacccacgt
     4261 tgcgggacac gtcggcaccg agggcaatga gcttgctgat cgcatggcca tgtacgccgc
     4321 gcaaaccaga gaaaaaaaac tgcgtcgata tcaagaaacc atggacattc cgacgctgct
     4381 caaaatgcgg gcgggttaag ccgccgcttc tttcgcgcgc acgccaacaa gaaactgcga
     4441 gggagtattt atgatggaga gcgctggttt ggtgctggat gcattggggc gcgtgcggga
     4501 tatggtgcgc gacgcactca ccgatttgtc ccctgagcaa ctgcttgcgc cgcctaaacc
     4561 tcacatcgcg tggttggtct ggcacatcgc gcgggttcag gacgccaatt tctcgaactt
     4621 gatggaacat ccgcaacttt ggatcgccga tggatggcac gatcgcttca acatggcgcc
     4681 ggatccgcga gattatggct ccggccatag gcacacgccg gcacaagtcg atgcctttgc
     4741 tgtgaccgaa acacaactgc tgatggacta tttggatgcc gtattcgcca ggaccaaagg
     4801 ctatcttgcc aatctgacaa atgaagatct aaaccgcgtt ctgaacgagc cacagtatca
     4861 gccgcttccg accctcagta tccggctcgc cagcgtgatc aactgcaaca cgcgccatgc
     4921 tgggcaaatc gaatatctcc gaggattggc caaatacggc gggtggtttc ctggcgctga
     4981 aaaaatgcct gcttagttga aaccgcagta gatcctactc gatgattggt gcgtcccatc
     5041 ggcggccgat ttctgaacag gacgaaccag cgatttaccc agtattagcc aagctgcgcg
     5101 atctcctgcc cccggacttg agctaatctg tagtccccgc acgggcgttg tttccgcaaa
     5161 aagtcgattt tttcgcctgt cattcctgtc acggagcctg gaactgtcag aatcaggtgt
     5221 cactattcct gagatttcga gatatctgct gacagcttga gtgcttccta tccctccaag
     5281 gagctaatcg tgcaggcctg gagcttgctc gaacgagatc gaatggagat ctcatctatg
     5341 cacacccaac gattccgcac caagaaaccg ctggctttga actgcaccgg acagggcatt
     5401 gtcgaataca cgctcatagt acttctagtt gcgatcgttt tttggctggg tatccgtgac
     5461 acacagttcg gattagccct gcaaacagca tggaatgagg tgggaagctc tatgtcgtcg
     5521 atgaacggac atccgggcag cgacgctgga ggaagcggct caacgggcgg ctcggatgcg
     5581 ggctcctcca atggcggttc cagcagtgga ggtggtgctg gagtcggcgg aagttccggc
     5641 agcggaactg gcagcggcac ttccggttca aacggcagtg gttcgggggc ttcggacggc
     5701 agttctgggg gaaccgtcgg tggtggctct ggcagcgggt ccggtgctgc ttcggctggc
     5761 ggctctgcag gtgcctccgg tggtggtgga tcaggctctt caggtggatc gggaggtttt
     5821 tccggtagta cgggtactgg ctccggcacg ggatccggcg gtaattcagc tggggggtct
     5881 tccggtggct ccagtggtgg ttcaggcttc tccggcggat cggcaggaag ctccggaagt
     5941 tccggtgtcg gctcccccag cggctctgcg ggtggtttcg ccggcagctc gggcgggggc
     6001 acggctggag gtccggatgg cggcggtagg tctgcagcga gttcgaccgg cggctcgagc
     6061 aatggtggag gcggtcgcgg ctcgggtgac gggtcaggga gcggcggatc cggtggtgga
     6121 tcgggcggcg ggtcgggcga tagcggctcg gctggtggct cggccagcgg cggctccggg
     6181 tctggcagca acggacaggt tagtttgatc gccgcggcga gcacgacgac aaccgctgaa
     6241 tccttaacag atcgttcacg aagcgcagca gcgatggaat cttcaactgc gggcgcgagc
     6301 ggcacagacg ataaacaaac agggaccggg agtggcgctg ctgacggatc tgcttcaggg
     6361 acgatttata cgttcggatt ctggctcgca cttctgtttg ctgcgctgag ttcactcttg
     6421 gttctgatct gggtcgtcgt caaagtcgtg ggcaaaaacg aa
//
LOCUS       DASULZ010000822         3088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_602775, whole genome shotgun sequence.
ACCESSION   DASULZ010000822 DASULZ010000000
VERSION     DASULZ010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602775"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1069)
                     /locus_tag="VFY96_16900"
     CDS             complement(86..1069)
                     /locus_tag="VFY96_16900"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5608207.1"
                     /translation="MPVATKAGWLFALILFSIVCPACRSEAIDDIRIAYPSTSFTTMP
                     ILAASKYGLYQHEGLRPELIFMRPNISVTAVVTGQVDFATAHGSIVRAAAQGMPVKSL
                     MVVADRPAYYLVARPSINSVAALRGRSVGIASLGGSVHLMTKEFLAQNNLDGDKNVVL
                     VVTGDHSTSIQALQNGHVDAAVLSVPWQTVAEKAGFRKLAYFGDVMRLPMAGLGASDE
                     NIAKKPDLLRRSVRATLRGIEFLRDVKNKKEVVGIMVGWFKVSTEQAQEAYGQMVEAY
                     PPNGMVANEVIEKDLEIARQTGAIKTRVPLSRVIDFQFVKAAGGELPVKKP"
     gene            complement(1072..1572)
                     /locus_tag="VFY96_16905"
     CDS             complement(1072..1572)
                     /locus_tag="VFY96_16905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608208.1"
                     /translation="MNADAANRAHEALIKSGVNFVACLPDSAFQELYLPLSADPKITY
                     VQVASENDGVGVCMGAWLGGMKPALLVENTGFTLGTYALLRGPMAFGIPMLLLISHRG
                     ELGDERWFSVPFGWGTKPLLDSMRITHRSVEKAEDVSAAIANAVKSMNSMQAPVAILF
                     AGEILF"
     gene            complement(1769..2386)
                     /locus_tag="VFY96_16910"
     CDS             complement(1769..2386)
                     /locus_tag="VFY96_16910"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5608209.1"
                     /translation="MSNDSSKPQRQEILAAIKPALKETDLIVSALAGTTADTYQAIDR
                     PGNLYLVGMGMVSQVALGLATALPKCRVFSFDTDGSMLLAPSILPVIGTRQPKNLYIV
                     VFDNEQLFGSRGGPRSQTATGTNIAGIAKASGVESVRTLRDKALLRSEMDAFLASEGP
                     SMLVAKIQALGRGAGPKMDGQENKYKFVRHVERIAQIEILGAPKA"
     gene            complement(2405..>3088)
                     /locus_tag="VFY96_16915"
     CDS             complement(2405..>3088)
                     /locus_tag="VFY96_16915"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5608210.1"
                     /translation="AGVSGVPLVTVAGIYNRTFFELIARRDIKSLTELKGKTVGISRY
                     GASTEYALRYGLKANGIDPDKQIKMLAVGEDAARIAGLQNGTLAAIVSQVPANFFAHK
                     VGGHTLLPLGDYLETLLAGVGVGQKKLEQNRDEVKRVVRALLHSLDFMYAQPAEVKGL
                     IRKRLRVSETEIVDHIYALVTKYATRNGIPSQRAIENTLLGTPFEGKVTNFDKLTDFS
                     MARELAQEN"
BASE COUNT          629 a          900 c          860 g          699 t
ORIGIN      
        1 ttcttttgcc gtcgtaagga taggccatca tagtcttcta cctccattga agcggacttg
       61 tgcgattcca gcccggcgtc gttacttaag gcttctttac gggtaactcg cctcccgctg
      121 ctttgacaaa ctgaaaatcg atcacccgag acaagggaac gcgagttttg attgcgccgg
      181 tctggcgcgc aatctcgaga tccttctcga tcacttcatt agccaccatg ccgttcggcg
      241 gataggcctc aaccatttgc ccgtaggctt cctgcgcctg ttcggtgcta actttaaacc
      301 atcccaccat gatgccgacg acttcctttt tattttttac gtctctgaga aactcgatgc
      361 ctcgaagcgt tgcgcggacg gagcggcgca ggagatcggg tttcttggcg atattttcgt
      421 ccgaagcgcc gagcccggcc atgggcaagc gcatcacgtc gccgaaatac gcgagttttc
      481 gaaagccggc tttttcagcc acagtttgcc atggaaccga gagcaccgct gcgtcgacgt
      541 ggccattttg caacgcctga atgctcgtac tgtgatctcc ggttacaacc aagacgacgt
      601 ttttatcgcc gtccaaatta ttctgcgcca ggaattcctt ggtcatgaga tgaaccgagc
      661 cgccgagcga agcgatcccc actgatctgc cgcgcagtgc ggcaacgcta tttatactgg
      721 gtctcgcaac caggtaatag gccggccgat cggcgacaac catgaggctc ttcaccggca
      781 tgccctgcgc cgcggcgcgg acgatggaac cgtgtgccgt agcgaagtcg acctgaccgg
      841 tgaccacggc tgtaacgctg atgttcggcc gcatgaatat caactcgggg cgcaggcctt
      901 cgtgttggta caggccgtat ttgctcgcgg cgagaatcgg catggtggtg aagctcgtcg
      961 atggatacgc gattctgatg tcatcgatcg cttccgagcg acaggccggg caaacgatgc
     1021 tgaataggat cagggcaaac agccatcctg cttttgttgc gacgggcaat gtcaaaacaa
     1081 aatctccccg gcaaaaagaa tggcgacggg cgcctgcatg gaattcatcg atttgacggc
     1141 gttagcgata gccgcggaaa catcttcagc tttttctacc gagcggtgcg tgatgcgcat
     1201 gctgtcgagc aacggcttgg tgccccagcc gaacggcacc gaaaaccagc gctcatcacc
     1261 caactcgccg cgatggctga tcaacagcag catgggaatg ccgaaggcca ttggaccgcg
     1321 caagagcgca taggtgccga gagtgaaacc ggtgttctcc accagcagag cgggtttcat
     1381 tccgcccaac caagctccca tgcagacgcc gacgccgtcg ttctcgctcg ccacttgaac
     1441 gtaggtgatc ttcggatccg ccgacagcgg cagatacaat tcctgaaacg ccgagtcggg
     1501 caggcaggcg acgaaattga cgccggattt gatcaatgct tcatgcgctc ggttggcggc
     1561 gtccgcattc attaattttt tcaccgttgt tgactgtaac agttgatgcg cgtctaagtt
     1621 cgaagaactc ttcgagaagc tcaggctgag cggtcaagtc ataagccgtt cgtggtgagg
     1681 ttctcaaacc atgaacggcg aacgacatgt aaacatgaca gttcactcgc tgtgacctac
     1741 agtgattgtg ctttcaatcg cgcgcagctc atgctttggg cgcaccgagg atctcgatct
     1801 gcgcgatgcg ctcaacgtgc cggacgaatt tgtacttgtt ttcctggcca tccatttttg
     1861 gccccgcgcc tcggccgagc gcctggatct tcgcgacgag catcgacggt ccctcgctgg
     1921 ccagaaaagc gtccatttcc gagcgcagca gagctttgtc ccgcagcgtg cgcacgctct
     1981 caacgccgga tgctttggcg atcccggcga tgttcgtacc ggtggcggtt tgacttctcg
     2041 gaccgccgcg gctgccgaaa agctgctcat tatcgaaaac cacgatatag agatttttcg
     2101 gctggcgcgt gccgatcacc ggcaagatgc tcggcgcgag caacatgctg ccgtcggtat
     2161 cgaaggagaa gacccggcat ttaggcaaag ctgtcgccaa accgagagcg acctggctga
     2221 ccattcccat gccgaccaga tacaaattcc ccggccggtc gatcgcctgg taggtgtccg
     2281 ccgtggtacc ggcaagcgcg ctgacgatca gatcggtttc tttcagagcg ggttttatgg
     2341 ccgccaagat ctcttgtctt tgcggctttg aactatcgtt gctcatggac accgccttct
     2401 cgttttagtt ttcctgcgcg agctcgcgcg ccatggaaaa atcggtcagc ttatcaaagt
     2461 tggttacctt accttcgaac ggggttccca acagcgtatt ctcgatcgcc ctttgcgaag
     2521 gaatgccgtt gcgcgtcgcg tatttcgtca ccagtgcgta aatgtgatcg acgatctcgg
     2581 tttcggatac ccgaagtcgc ttgcggatca gccctttcac ttccgccggc tgagcataca
     2641 taaaatccag cgaatgaagc agcgcgcgaa ccacgcgttt cacctcgtcg cggttctgct
     2701 cgagcttttt ttggccgacg ccgacgccgg ccaacagcgt ctccaaatag tcgcccaaag
     2761 gcaacagcgt atggccgccg actttgtgcg cgaagaagtt tgccggcacc tgcgagacga
     2821 tcgcggcgag cgtcccattt tgcaatccgg cgattctcgc ggcgtcttcg cccaccgcga
     2881 gcatcttgat ctgcttgtcg ggatcgatgc cgttggcctt gaggccgtat cggagcgcgt
     2941 actccgtcga agcgccgtag cggctgatcc cgacagtttt gcctttcagt tcagtgagag
     3001 atttgatgtc gcggcgggcg atcaattcga agaacgtgcg attataaatt ccggcgacgg
     3061 tgaccaacgg gactccgctg acgccggc
//
LOCUS       DASULZ010000823         4062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_297632, whole genome shotgun sequence.
ACCESSION   DASULZ010000823 DASULZ010000000
VERSION     DASULZ010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297632"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1102
                     /locus_tag="VFY96_16920"
     CDS             191..1102
                     /locus_tag="VFY96_16920"
                     /inference="COORDINATES: protein motif:HMM:NF017809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HEX5608211.1"
                     /translation="MNRRANANVLGQNYADFDLSYRRPYDWQSILRFYQSHSITGIER
                     VSEHYFERLFRIKQRLGLVRVQAGKNCLSVRVAPEAECIVTEVKTRVRKMFDLDCDPR
                     LIVASFGRTPLLKKLCKRFPGLRLPRGWDPFETAICTILGQLVSAGNRAALIGQLVQN
                     YGEEIVNPFSGENVRLFPTPQVLAEAELNAVKTTRARRVAIRDFSRRILKGALSLTDY
                     QEPAALRKALLDTKGIGPWSAEYISMRAVGDTDAFPRTDLILNRVLALYPALDLTAVK
                     PWRSYAAMYLWKAFAQRLSKQRKEPQP"
     gene            1099..1599
                     /locus_tag="VFY96_16925"
     CDS             1099..1599
                     /locus_tag="VFY96_16925"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX5608212.1"
                     /translation="MSLFYKEIKSPVGKLKLIASAHALVAVLWDLEGPDRVKLDAPTF
                     APEHPILIETERQLNEYFTGARAEFDLPIDPHGSAFQKKVWRALRKIPFGETRSYLDL
                     AKALGSAAASRAVGLANRKNPLSIIVPCHRVIGSDGSLTGFAGGLETKAALLALEAKA
                     AAATRD"
     gene            1602..2312
                     /locus_tag="VFY96_16930"
     CDS             1602..2312
                     /locus_tag="VFY96_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002635770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX5608213.1"
                     /translation="MRLSASDREGVLWEAACGELDKRGFALIPALLSPESCDEVAGYY
                     ADDRRFRSRVEMARYAFGRGEYKYFAYPLPKIVQQLRNSIYPELAPLANQWAERLGTH
                     VRYPKRLSDFLEQCHRAGQKRPTPLVLKYGTGDFNCLHQDLYGEIVFPFQLTVFLSQR
                     GKDFDGGQFILCEQRPRQQSRVEALDPYRGDAIVFSVRHRPVRGLRGYYRATVRHGVS
                     TVHSGERYTLGIIFHDAK"
     gene            2443..3627
                     /locus_tag="VFY96_16935"
     CDS             2443..3627
                     /locus_tag="VFY96_16935"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX5608214.1"
                     /translation="MLTQQENSFLTRIGPGTPAGQLLRRYWHPIAAAVELTEEKPKKR
                     VRVLGEDLLLYRNQNGGYGLVRERCSHRGASLYYGFVEADGIRCAYHGWKYDAGGKCV
                     EQPFENPETGFRDKIRHTAYPVEKLAGLLFTYMGPPEKQPLLPKWDLLVRPDGLKKID
                     VCEVLRCNWLQAMENSVDPTHTYYLHSHTLKLKGSKDYVPFHYQQLNKIEFELVIQPN
                     WAGIQKQRIFAGDNVPLEAPHPLIFPNILFVPVRTGYAMHFRTPIDDANTQVFQFRFN
                     PTNDGSIPEQPDDSPMEFIATKDDTGEFHLSHFASQDHMAWETQGPIADRTDEHLGEA
                     DRGVIMFRKLLHEQISAVQNGQDPIGVNFDPVKDQTIELIHEGYDAYSFAQKRQSADE
                     RA"
     gene            3758..>4062
                     /locus_tag="VFY96_16940"
     CDS             3758..>4062
                     /locus_tag="VFY96_16940"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5608215.1"
                     /translation="MEEELSRSIDAPAIELQSQPARFHIFSSLRHLDYRYLWSGTLMM
                     SAGQWVQQVTLGWLLYDMTGNSMLLGALNGLRAVPFLVTGPMAGVAADRMDRRKLLL"
BASE COUNT          893 a         1155 c         1093 g          921 t
ORIGIN      
        1 cctgccacac cactgcgcga gtggaccgga cgaagatcat gtttttcgcc gatcctcgag
       61 aagcgaaaca ggccggcttg cggccgtgca aaatttgccg gccggcgtag agtacccaca
      121 gatcgaaact ttgcgggcgt atagtgtaga ccttttctgg actggaaatt tacgggagaa
      181 gtgagcgctc atgaatcgac gagcgaacgc aaacgtcctc gggcaaaact acgccgattt
      241 cgatctgtcc taccggcggc cttatgactg gcagagcatc ctgcgctttt atcagtcgca
      301 ctcgattacc gggattgagc gtgtaagcga acattatttc gagaggctat ttcgtatcaa
      361 acagaggttg gggcttgtcc gagttcaagc cggaaaaaat tgtctgagcg ttcgggtcgc
      421 tcccgaggcc gagtgcatcg tgacagaagt aaaaacccgt gtacggaaga tgttcgatct
      481 cgactgcgat cccaggctga ttgtggcaag ctttggccgc actccgctat tgaaaaaact
      541 ctgcaagcgt tttccgggat tgagattgcc gcgcggctgg gacccttttg agaccgcgat
      601 ctgcacgata ctgggccaat tggtctcagc cggaaaccgc gccgccttga tcggccagct
      661 cgtgcagaat tacggcgagg agatcgtcaa tcctttctcg ggagaaaacg tccgtctgtt
      721 tccgacgccc caggttcttg ctgaagccga gctaaacgcg gtcaagacca cccgggcccg
      781 ccgcgtggca atccgagatt tcagccggcg catactaaaa ggcgctcttt ctctcactga
      841 ttatcaagag cccgccgcat tgcgtaaagc gctgctcgat acgaaaggga tcgggccatg
      901 gtcggctgag tacatcagca tgcgcgccgt tggcgatacc gacgcgtttc ctcgaacgga
      961 tttgattctg aaccgcgtac tcgcgcttta tcccgccctg gatttgaccg cggtcaaacc
     1021 ttggcggtct tatgccgcga tgtatctctg gaaagcattc gcgcaaaggc tttccaagca
     1081 aagaaaggag ccgcagccat gagcttattt tacaaagaaa taaaatcgcc ggtgggcaaa
     1141 ctcaaactga ttgctagcgc gcacgcactc gtcgcggtcc tctgggacct agaagggcct
     1201 gatcgcgtca aactcgatgc gccgaccttc gcgcctgagc atccgatcct gatcgagact
     1261 gagcgccaac tgaacgaata tttcactggc gcgcgagccg agttcgacct tcccatcgat
     1321 ccgcacggca gtgcgttcca aaagaaagtc tggcgagctt tgagaaaaat acctttcggt
     1381 gaaacccgga gctacctcga tctcgcgaag gcgctcggct cagccgctgc ctctcgtgcc
     1441 gtaggcctgg ccaaccgcaa aaatccgctg tcgattatcg tgccatgtca tcgggtaatc
     1501 ggctcggacg ggtcgctcac cgggtttgcc ggcggacttg aaaccaaagc cgcgttgttg
     1561 gcgctcgaag cgaaagcagc ggcagcgacg cgggattagc gatgagactt tcagcgagcg
     1621 atcgcgaggg cgttctctgg gaggccgcgt gcggcgagct cgataagcgt ggcttcgcat
     1681 tgatcccggc cctgctttcg ccggaaagtt gcgacgaggt cgccggctac tacgccgacg
     1741 atcgccgctt tcgctcgcgc gttgaaatgg cgcgctatgc tttcggccga ggtgaatata
     1801 aatatttcgc ctatccactt cccaagatcg ttcagcagct caggaattcg atctatcccg
     1861 agctggcgcc gctggccaat cagtgggccg aacggctcgg gacccatgtt cgttacccga
     1921 aacggctttc ggacttcctc gagcagtgcc atcgcgctgg tcaaaaaagg ccaacgccgc
     1981 tggttttgaa atatggcacc ggcgatttca actgtttaca tcaagatctc tatggcgaaa
     2041 ttgtttttcc atttcagctt accgtttttc taagtcagcg cggcaaagat tttgacggcg
     2101 gccagtttat cctttgcgag caacgaccgc ggcagcagtc ccgcgttgaa gcgctcgacc
     2161 cctatcgcgg tgacgcgatc gtcttttccg tccgccatcg cccggtccga ggattacgcg
     2221 gctactaccg cgccaccgtc cgccacggcg tcagcacggt tcattcaggc gagcgctaca
     2281 cgttgggaat cattttccac gatgcgaaat agcttgactc cattcttttt cgcgccgagc
     2341 ttttgcgaaa ggcgctttcg ttgacacttc cttgggagtt cgattactgt caatttattt
     2401 gactcggcga actccttgat catttgtgta aggagccaac ccatgctcac tcagcaagaa
     2461 aatagttttt tgacccgaat cgggccagga acaccggccg gccaactgct caggcgctat
     2521 tggcatccga tcgctgcggc ggtggagctc acggaggaaa aaccaaaaaa acgagtgcgc
     2581 gtacttggcg aagacctgtt gctctatcgt aaccaaaacg gcggctatgg tctggttcgc
     2641 gagcgctgtt cccatcgcgg cgcctcgctt tactacggct ttgtcgaagc tgacggcatt
     2701 cgctgcgcgt atcacggttg gaaatacgac gctggcggca aatgcgtcga acaacctttt
     2761 gagaatcctg aaaccggttt tcgagacaaa atccgccaca ccgcttatcc ggtcgaaaag
     2821 ctcgccggtc tattgttcac gtacatgggg ccgcccgaga aacaaccgct cctgcccaaa
     2881 tgggacttgt tagtgcggcc cgacggtctc aaaaagatcg atgtctgcga agtactacgg
     2941 tgcaattggc ttcaggccat ggagaactcg gtcgatccga cacatacgta ctatctccac
     3001 tcgcacacgc tcaaactcaa gggcagcaag gattatgtgc cgtttcacta tcagcagctg
     3061 aacaaaatcg aatttgagct cgtcatccag cccaattggg caggaatcca gaaacaacga
     3121 atttttgccg gcgataacgt acccctcgag gcgccgcacc cgctcatctt cccgaacatt
     3181 ctcttcgttc cggtgcgcac cggatacgcc atgcattttc gcacgcccat cgatgatgcc
     3241 aacacgcagg tttttcagtt tcgtttcaat ccaaccaacg acggcagcat tccggaacag
     3301 ccggacgatt cgccgatgga attcattgcc accaaagacg acaccggcga atttcatttg
     3361 agtcattttg ctagccaaga tcacatggct tgggagaccc aaggcccgat tgctgatcgt
     3421 accgacgaac acctaggcga agccgaccgc ggcgtcatta tgtttcgtaa gcttctgcac
     3481 gagcaaatca gcgccgtcca gaatggccag gatccgatcg gcgtaaattt cgatccggtg
     3541 aaggatcaga ccatcgaact gattcatgaa gggtatgatg cttattcctt tgctcaaaag
     3601 agacagagcg cggatgagcg tgcttaaccg gcgcgtgtcc aggcggttct ttggacgtct
     3661 gtttgttcgg caaacacaat atcattcgtg gtaagcgtgt ttaaatcttg ctaaaattgt
     3721 cttaacctcg cgaccgtact cacaggaact taagcttttg gaagaagaac tctctcgctc
     3781 gatcgacgca ccggcgatcg agcttcagtc ccagccggct cgttttcata ttttttcttc
     3841 tcttcgccac ctggattatc gttatctctg gtcgggcact ctgatgatga gcgctggtca
     3901 atgggttcag caggtaaccc tcggttggct gctctacgac atgaccggca attcgatgct
     3961 gctgggcgcg ctcaacggct tgcgcgccgt gccgttcttg gtcaccggac cgatggccgg
     4021 ggtggcggcc gatcgcatgg atcgccgcaa gctattactc ag
//
LOCUS       DASULZ010000824         2545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_805219, whole genome shotgun sequence.
ACCESSION   DASULZ010000824 DASULZ010000000
VERSION     DASULZ010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805219"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="VFY96_16945"
     CDS             complement(<1..1109)
                     /locus_tag="VFY96_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016858522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5608216.1"
                     /translation="MMTTEVSKLKEKLEGCIKGSVLVPNDPAYEEARTIWNAMIDRRP
                     TMIVQCADADDVAPAIDFARRHRLELSIRGGGHNIAGSSLCDHGLTIDFSKMKSVRVD
                     AERKRAYVEPGATLADLDEATQAHRLATPVGINSTTGIAGLTLGGGFGWLTRKYGMTI
                     DNLVAVDMITVDGKKRRASKSENEDLFWAIRGGGGNFGVVTQFEFELFPVGPEIFAGL
                     MVFPFSQAKQILRKYREFVNSAPEELNVWVVLRKAPPLPFLPENVHGKEVVVLPIFYS
                     GPISAAEKLIAPLRDFGQLHGEHVGPQPYIQWQKAFDPLLTPGARNYWKSHNFTELAD
                     GVLDSIIEFAGKLPSPQCEIFIGLIAGAPNRIGPDA"
     gene            complement(1153..1527)
                     /locus_tag="VFY96_16950"
     CDS             complement(1153..1527)
                     /locus_tag="VFY96_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5608217.1"
                     /translation="MSVAETTNSKRVKDVLDHHLKCFGEGDLKGILSDYAPSAVLFTS
                     DGPLKGTDAIRPLFQAMIGEFGKPGAAFRMKRQFVEGEYAYILWSAETADNMYEIGTD
                     TFVVRDGKIVAQSFTGKITPKG"
     gene            complement(1557..2468)
                     /locus_tag="VFY96_16955"
     CDS             complement(1557..2468)
                     /locus_tag="VFY96_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017326920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608218.1"
                     /translation="MKNNYSYQYALEVSERIGWRVEDIIGGEKRLDFNRPFMPESLAQ
                     VKQLSFLTPDEQRILNQIRGYEYLAMFGLVEEFILPYVVDYARLQLSGDDYRVRALLQ
                     FAGEEAKHIHLFKRFRQEFEAGFGVKCDFIGPAEDVKRFVLSHSPLGVGLAILHIEWM
                     TLRHYIEGVKDNQDLDPQFKSLLKHHWLEESQHTKLDTLIVEALAEMAVEKEIEQAFR
                     EYADIGGFLDNGIKQQTEFDLESFVQATGRNLSQSEREAMTATVLKGMRWTYLGTGMT
                     HPNFLGTVEQISPEGKKQIEAMAPMFC"
BASE COUNT          560 a          735 c          626 g          624 t
ORIGIN      
        1 gcatccggtc cgattcgatt gggcgcgccg gcaatcaggc cgataaaaat ttcgcattgt
       61 ggagacggta acttgccggc aaactcgatg atggaatcca gcacgccgtc agcgagctcg
      121 gtaaaattgt gagacttcca ataatttcgc gcaccgggcg taagcaatgg atcgaaagcc
      181 ttttgccact gaatatatgg ctgaggaccc acatgctcgc cgtggagttg gccaaaatca
      241 cgcaaaggag caatgagctt ctcggctgcg gagattggac cggagtaaaa aatcggcagc
      301 acgacgactt ccttgccgtg aacgttctcg ggaagaaagg gcaacggcgg agccttgcgc
      361 aacaccaccc agacgttgag ctcttcaggc gcagagttta cgaattcacg gtactttctc
      421 aagatctgtt tcgcctggct gaacggaaaa accatcaagc cggcaaatat ctcaggaccc
      481 accggaaaaa gctcgaactc aaactgcgtt accacaccaa aattgccgcc tccgccgcgg
      541 atcgcccaga agaggtcctc gttctcactt ttgctcgctc gtcttttttt gccgtccacg
      601 gtgatcatgt cgaccgcgac gagattgtcg atcgtcatgc cgtatttgcg ggttagccag
      661 ccaaaaccac caccgagcgt gagtccagcg ataccggtgg ttgaatttat tcccacgggt
      721 gtggcgagac ggtgcgcctg ggtcgcctcg tccaagtccg cgagcgtcgc tcccggctct
      781 acataggcac gctttctttc tgcatcgacg cggacgctct tcatttttga aaaatcgatt
      841 gtgagaccat gatcgcagag cgagctgccg gcgatgttat gtcccccgcc gcgaattgag
      901 agttcgagtc tgtgacgccg cgcgaaatcg attgccggcg ccacgtcatc agcatcggcg
      961 cactgaacga tcatcgttgg ccggcggtcg atcatcgcgt tccagatcgt gcgggcttcc
     1021 tcgtaagctg ggtcgttggg aactaacacg ctccctttaa tgcatccttc aagcttttcc
     1081 ttcaacttcg acacttctgt cgtcatcata ttcgtctcct cgttaaattt tgtcaccgga
     1141 cgaaggcgtg tttcagcctt tcggtgtaat cttgccggtg aatgactgtg ccacgatctt
     1201 gccgtcccgt acgacgaatg tgtccgtccc tatctcgtac atgttgtcgg cagtctctgc
     1261 gctccacaga atgtaagcat actcgccttc gacgaactgc cgtttcatcc taaaagctgc
     1321 accaggtttt ccaaactccc caatcatcgc ctggaacaac ggcctgatcg cgtcggttcc
     1381 cttaagcggt ccgtccgacg tgaacaggac ggcactcggt gcatagtcgg atagaatgcc
     1441 cttgaggtcg ccctcgccaa aacactttaa gtggtggtct agaacgtctt tgactctctt
     1501 gctgttcgtt gtttctgcga ctgacatagc tcctccagaa cttttttgcg ccttactcaa
     1561 caaaacatcg gtgccattgc ctcaatctgt tttttcccct cgggcgagat ctgctcgacg
     1621 gtgcccagaa agttcggatg cgtcatgccg gtgcccagat aagtccagcg cattcctttg
     1681 agcaccgtcg cggtcatcgc ttcacgctcg ctttgcgata gatttctacc tgtggcttgc
     1741 acaaagcttt ccaggtcgaa ctccgtttgc tgcttaattc cgttgtcgag aaacccgccg
     1801 atgtcggcat attctcgaaa ggcctgttcg atttccttct cgacagccat ctccgccagc
     1861 gcctccacga ttaacgtgtc gagcttggtg tgctgtgatt cttccagcca atgatgcttt
     1921 aaaaggctct tgaactgtgg gtccagatcc tgattatcct tcaccccttc gatgtagtgc
     1981 ctgagcgtca tccattcgat gtgcagaatg gcaaggccaa ctcccaacgg actgtgcgac
     2041 aaaacaaacc gcttcacatc ctctgcggga cctatgaagt cacacttaac gccgaaacca
     2101 gcttcaaatt cctggcggaa gcgcttgaaa agatgaatgt gctttgcctc ctcgccggca
     2161 aactggagta gagcgcggac gcgatagtcg tctcccgaga gctgcaatcg cgcatagtca
     2221 accacgtaag gaaggatgaa ctcttcgacc aggccaaaca tggccaagta ctcgtacccc
     2281 cgaatttgat tcaaaattcg ctgctcgtcg ggcgtcagaa aagaaagctg ctttacctga
     2341 gccagcgact ccggcatgaa gggcctatta aaatcgagcc gcttctcgcc accgatgata
     2401 tcttccactc tccagccgat ccgctcggat acctcaagcg cgtattgata gctataattg
     2461 tttttcatga tcgatcctcc tgaaatttgt ttgctgttag tcgtaatctg tcccgcgcat
     2521 agtgctgaaa ccatgccagc gagac
//
LOCUS       DASULZ010000825         4214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_704528, whole genome shotgun sequence.
ACCESSION   DASULZ010000825 DASULZ010000000
VERSION     DASULZ010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704528"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="VFY96_16960"
     CDS             <1..1080
                     /locus_tag="VFY96_16960"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX5608219.1"
                     /translation="FNFRYVTNMMGKAEMANYCFFPKEGDPAVVTRPGHSLAGAMLAR
                     TAVRNVIVGKPSVLDAIVAEIRERRYDQGRIGIVEYDPYTSIPKNHWDHFTTNLAQAE
                     LVPVTKEFIAMRLLKSPEEILFLDRSAELGDIGIRAMQKRVKPGMSEGEVFGLVHDAV
                     LKAGGEMGMIQLGSCSMLDPDINDQRPRPVERIIGERDIINNELGIFYNGYEAQCGRP
                     VTTGRPTSEYREMFDLAIEGYRRLVPTLHVGKSSEDSIEAMQFIRDTEYEFYGGFLQG
                     MLGANPRHEPQIGFDRVQSAEDRYLFDSGGRFIYQVGHVFTLQMHIVDKSHTRGLFVG
                     DFFVIEENGPRCLNKLPLEMVRTQI"
     gene            complement(1262..1654)
                     /locus_tag="VFY96_16965"
     CDS             complement(1262..1654)
                     /locus_tag="VFY96_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608220.1"
                     /translation="MIRNVVFVAIIALFWGIASAQLLEDRVRELERRVEQLEKQQSDR
                     QQPPFGAPRKATPAAPDGWRSMANWRTLKRGMTENEVRSVLGEPQKIDVGPLLTVWYY
                     DYPRGGTVRLSSSDRRVEGWTEPRDTDK"
     gene            2784..3491
                     /locus_tag="VFY96_16970"
     CDS             2784..3491
                     /locus_tag="VFY96_16970"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HEX5608221.1"
                     /translation="MQRFCLNRWTAGRWIALGAAALFGLVAAASADRMVIIEDWTNNR
                     VSQRGIPSGWTGEAFGRQADYDFSIEQAGEARVLHVKSNNEHSTIVKDITGMVRLKET
                     PILEWTWKVSILPMGGDVRRKESTDIASQLYIIWPRFPALLRSRIIGYIWDATTPAAT
                     IVKSQKTGTVTFIVIRSGTKDLGKWLTERRNVAEDYRALFGEFPEDPTGITISIDSND
                     TRSSAESFMGPIVFREK"
     gene            3631..>4214
                     /locus_tag="VFY96_16975"
     CDS             3631..>4214
                     /locus_tag="VFY96_16975"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5608222.1"
                     /translation="MQNQLRRSLVNLLVLGIVNCFSLAGPARGDESIKFALPGNSMGY
                     LPLFVAVHRGFFKDEGLEIELPRFIPAIAQNALLAGEVQYHGLADSGLRLAARGLPIK
                     AIFYGADRPMYYLVAQKEIRSVPELRGKRIGVSQFGGTSDLAARLALKHFGVEPEKDA
                     LLIQIGAEGTRMAALRAGSVSAIIVPVPAVAILRR"
BASE COUNT          964 a         1090 c         1207 g          953 t
ORIGIN      
        1 ttcaatttcc gctacgtcac caacatgatg ggcaaggcgg agatggcgaa ctactgcttt
       61 ttcccgaaag agggtgatcc ggccgttgtt actcgccccg gccattcgct ggcaggcgcc
      121 atgctggcac gcacggcagt acgcaatgtg atcgtcggca agccgagcgt cctcgatgcg
      181 atcgtggcgg aaatccggga gcgccgttat gaccaaggtc gcatcggcat cgtagaatac
      241 gatccgtata cgtcgatacc gaaaaatcac tgggatcatt tcaccacgaa tttggctcag
      301 gccgaattgg tcccggtcac caaagaattc atcgccatgc ggctgctaaa gagcccggag
      361 gaaattctgt ttctcgatcg ttccgcagag ctcggcgaca tcggcattcg tgcgatgcag
      421 aagcgtgtga agcccggcat gagcgaaggc gaagtcttcg gtctcgtgca cgacgcggtc
      481 ctgaaagcgg gcggcgagat gggcatgatt caactcgggt cgtgctcgat gctcgacccc
      541 gacatcaacg atcagaggcc gcggccggtg gagcggatca tcggcgagcg cgatatcatt
      601 aacaacgagc taggcatctt ttacaacggc tacgaggcgc agtgcggcag gccggtgacc
      661 accgggaggc caacctccga ataccgagag atgtttgatc tcgcgatcga aggctatcgg
      721 cggctcgtgc cgacgcttca cgtcgggaag agcagcgaag attccattga ggcgatgcag
      781 ttcatccgcg acacggagta tgaattttac ggcggctttc tccagggaat gctcggcgcg
      841 aacccccgcc acgaaccgca gatcggcttc gatcgggtgc agagcgccga agatcgttat
      901 ctttttgact ccggcgggcg attcatttac caagtgggtc acgtttttac tctgcagatg
      961 catattgtcg ataagagcca cacgcgcggt cttttcgtcg gcgatttctt tgtcatcgaa
     1021 gaaaatggac cacgctgttt gaacaagttg ccgctggaga tggtccgaac acaaatttag
     1081 ggcgcatcgt gccgggttca cttcgtgcgt aatttatcgt actcggcctg cccgcgaatc
     1141 tcagaacgac caggggggaa acggatgtcg aactggctct tctcgtttgg cagagaagct
     1201 gcagcggcga gccaatgcat gagcaagaag cagcagcgtg catggctgga gtgaagtaaa
     1261 cctacttgtc agtgtcgcgg ggctcagtcc agccctccac tcggcgatcg ctgctgctaa
     1321 gccgtacggt tccaccgcgc gggtagtcgt agtaccacac agtgagaagt ggaccaacgt
     1381 cgatcttttg cggctcaccc aaaacggaac gaacttcatt ctctgtcata ccgcgtttaa
     1441 gcgtgcgcca gttggccatg gagcgccagc catcgggagc tgcaggagtt gccttgcgtg
     1501 gtgcaccgaa agggggctgt tgccggtccg attgttgttt ttcgagttgt tctacgcgcc
     1561 gttcgagctc acgaacacga tcttctaaca actgcgcaga tgcgataccc cagaacaacg
     1621 caatgatcgc gacgaaaact acgtttctga tcattaacgg ctctcatcat tgttcagtcg
     1681 gaataagtct acccgtcagc gttggggttg ccttatcgta cggaccatgg ttctggcctg
     1741 gtgcgcagcc gtttggaaaa gaccaaggtt tcctcgtgga agagcagctt tgtttgaata
     1801 ctcacattcc ggaaaatgat cgtcgaggta tctcgattct ttttccgcga gccgctgcac
     1861 ttcattcaga aatgagaggt ctctaagcct ctgcccgcat cgcagagcga ccaagagtac
     1921 ctgataagct attccgtact cgccgtcagc aaaggtggcg cggtttattt ccaaagattg
     1981 tcgataaatc tctcgaatgt ggccgtcgtt catagcagcg tctcccggtg tccggtgtac
     2041 tgaacagatg gatgcgaaag tctacgtttg ctgacatgac gatattccat cgtcgagcgg
     2101 ctgaagcaga actggagccg ggatacgggg tagtgtaacg ggggagcgtg atctgcgaag
     2161 gaggacttta tccgtatacc ggctccatcc aacagaaaaa gcgcttagat acaacgcaat
     2221 tttaggaccc tacccaaact ctaagttttt tgctgtcggt ccatttctcc tgtctgctaa
     2281 ataccaccag tgactgatca tcttgctagt gaatcgctga caatgattat ttaactaagc
     2341 gctcgggcaa tgtgcggcaa accgcatgac caacttgccc tgataccgcc aagccaatca
     2401 gtaaagcgag gttttgctgg tattgcaatt ataatcgtaa agcctgactg accctttctc
     2461 cctcccgtga cggttcgaca cgctaagatg cttcaagatt tgcgtcgact atggaatgcg
     2521 cgaaagatcg cgtcaaagaa tctaaattgg agccgtccag atcgagaata ttgaaagcga
     2581 tgaaggcaaa tacaaagggc atgcacgcgg tgcgccgaag gtttgaggta aatccgaatc
     2641 ccagtagctt caccgaagtc gtcgaacctt tcagtgatcg cctctttagg ctgcccgtct
     2701 ataatattga aacgagcatt ctgtcaatgg gtgtgactcc ataataaggc tagaatcagt
     2761 ggaacgatcg aggccgatta gctatgcaac gtttctgtct taatcgttgg acggcgggaa
     2821 gatggatagc tctcggagct gcggcgctct tcggccttgt cgcggcagcc agtgctgatc
     2881 gcatggttat catcgaggac tggacaaata atcgggtaag ccaacgagga attccctctg
     2941 gctggaccgg cgaggctttc ggacggcaag ccgattatga ctttagcatc gagcaagcgg
     3001 gcgaggctcg tgttctccat gtcaagagca ataacgagca ttcgacaatt gtaaaagata
     3061 ttaccggcat ggtgcgcctg aaagaaaccc caattcttga gtggacgtgg aaagtgagca
     3121 ttctgccgat gggtggcgac gtgcggcgca aagagtcaac ggatattgcg tcgcagcttt
     3181 atattatatg gccgcgcttc ccggcactgc tgcgctcgcg gattatcggc tatatctggg
     3241 atgcgacgac tccggcggct acgatcgtga aaagccagaa gaccgggacg gtgacattta
     3301 tcgtgatccg gtccggaaca aaagatctcg ggaagtggct gactgaacgt cgcaatgtcg
     3361 ctgaggacta cagagcacta ttcggcgaat tccccgaaga tccaacaggg atcacgattt
     3421 cgattgactc gaacgacacg cgctcaagcg ccgaatcgtt tatggggccg attgttttcc
     3481 gagaaaaata aaagcctctc agttcattcc tgctgattgt cttgtgcgtg gcgtagcctc
     3541 tccagaattt ggatctaacg aaagattaag ctaaaagggg cgcagtaaga atatcggcct
     3601 gattacctca cgattggaga aagtagtcgt atgcagaatc agcttcgtcg ctcgctggtt
     3661 aatttgctgg tccttggaat agtaaactgt ttctcgcttg cgggacccgc gcgcggcgac
     3721 gagtcgatca agttcgcgct gccggggaat tcgatggggt atctgccgtt gtttgtcgcg
     3781 gtgcaccgag gattcttcaa ggatgagggg ctcgagatcg agctaccgcg cttcattccc
     3841 gcgatcgcgc agaacgcatt gctcgccggc gaggtgcagt atcacgggct cgccgattcg
     3901 gggttgcggc tcgccgcccg cggactaccg atcaaggcga ttttttatgg tgccgaccgg
     3961 ccgatgtatt acctggttgc acaaaaggag attcgctcag ttcccgagct gcgcggcaag
     4021 agaatcggtg tcagccagtt cggcggcacg tcggatctgg cggcgcgctt ggcgctgaag
     4081 cattttggcg tcgaaccgga aaaagatgcc ctgctgatac aaatcggcgc ggaaggtacg
     4141 cggatggccg cgctgcgcgc cggctccgtc agcgcgatca tcgtgccggt gccagccgta
     4201 gcgatcctca gacg
//
LOCUS       DASULZ010000826         2631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_805482, whole genome shotgun sequence.
ACCESSION   DASULZ010000826 DASULZ010000000
VERSION     DASULZ010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805482"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..606)
                     /locus_tag="VFY96_16980"
     CDS             complement(25..606)
                     /locus_tag="VFY96_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608223.1"
                     /translation="MNKKQILEEDAVKTYNQFKVFEGKKYTGMKVGGRHKWYYDKGEW
                     KERKVAPDRWEFSYAVPKRRAGHAPVGSGVPVGTEYHWYILAHQNVRKLDANTYTTAL
                     TGLKYKLAHKRAGKDTWSASEEAQRRQLIKILQEMIDDLSSAKPERSAAGAKSEEQEA
                     DSQQRKRSHRRHAARESAPTPRRLVAAGGRSRR"
     gene            complement(921..2033)
                     /locus_tag="VFY96_16985"
     CDS             complement(921..2033)
                     /locus_tag="VFY96_16985"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HEX5608224.1"
                     /translation="MTTSTVVRLAGVLAIVVCIDAQGTRVKAQQRGGTAETDKVPPVN
                     SGPNPYRAVRDWAQLTLEKRPWGGSNGVAIDRDGKSVWATDRCSPGIAPGCLGTRANP
                     VHLFDETGKEIRSFGGGTFVWPHGIHVDREGNVWVTDARIPTAEERTQYPGEEKKGSV
                     VMKFSPDGKVLMTLGKPGVKGDPPDALTEPNDVVTDPNNGDIYIAESHTNVDDPKLVG
                     RISVFDRDGKFLRMIGKTGTGPGEFRTPHAIEFDSQGRLIVADRHNHRVQILTKEGKF
                     LAEWREFSRVSGLAIDPNDTIYAADSESDLKRHPDWLKGIRIGSLKDGKVIIFVPPHK
                     TETPDGAMGEGIAVDSAGNLFTAEATIRGITKYVRE"
     gene            complement(2145..>2631)
                     /locus_tag="VFY96_16990"
     CDS             complement(2145..>2631)
                     /locus_tag="VFY96_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEX5608225.1"
                     /translation="GMRGILSLAAAMALPAEFPYRDLIVLTAFSVVLGTLVVQGLTLK
                     PLLRALDLNDDDPVGRELNTARERALRAGLGSFAEDRSPVAELVRREFTARLANERAE
                     ADARDITRSNHSDIYRGALQAARQAVLEMRANDEIGDDAFHLMEEELDWLEMAGAREE
                     D"
BASE COUNT          482 a          812 c          712 g          625 t
ORIGIN      
        1 ttattaagca tcggacaccg ggggttagcg acggctccgc ccgccggctg caaccaggcg
       61 ccttggagtt ggcgcgctct cgcgtgcggc atgtctgcgg tgtgagcgct tccgctgctg
      121 cgagtcagct tcctgttcct cggacttggc gccagcggca ctgcgctcgg gcttcgccga
      181 gctcaagtcg tcaatcatct cctgtaagat tttgatgagc tggcggcgct gtgcttcctc
      241 gctggcgctc caggtatctt tgccagcgcg tttgtgtgcg agtttatact tgagtccggt
      301 aagggccgtg gtgtaggtgt tcgcgtcgag cttccgcacg ttctgatgcg cgagaatata
      361 ccagtggtat tcggtcccga cgggcacccc ggagcccaca ggagcgtggc ccgcgcgtcg
      421 cttcggcaca gcatagctga attcccatcg atccggcgcg acctttcgct ccttccactc
      481 gcccttgtcg tagtaccact tgtggcggcc gccgactttc atgccggtgt atttttttcc
      541 ttcgaagact ttgaactggt tgtaggtctt caccgcatct tcttctaaga tttgtttctt
      601 gttcatggtg ccccgctcga atatagccga ccacgcggaa tgacgttatt gcgcaatctt
      661 ttactcgtag gaattcagca aaacaaatgc cacgcaaatg ccgtcttaaa attcgaactc
      721 tccggcgaga cctgtctcat tctcgcgaaa gttacggcgt ctgagtggtc attcgatcag
      781 acactcccgc tagatcaact caggcagaag aaatcagttg tgttctgatt ggcaccggcg
      841 tgtgaacaat ccaaacgact tcatcttact acagtacaga atcttctcgc gaaaagcggc
      901 agcgattctc ttcgcgtcgt ctactccctg acgtacttcg ttatgcccct gattgtggct
      961 tctgccgtaa agagattgcc ggcactatcg acggcaatcc cctctcccat cgctccgtcg
     1021 ggcgtttcgg ttttgtgcgg cggcacaaaa attatcacct tgccgtcctt caagctccca
     1081 atacgaatgc ccttgagcca gtctggatgc ctctttaaat ccgattcaga atctgccgca
     1141 taaattgtgt cgttcgggtc tatcgccagc ccgctcacgc ggctgaattc gcgccattca
     1201 gcaagaaact tcccctcctt ggtcaggatc tgaactcgat ggttgtggcg gtcagcgaca
     1261 atcagccgtc cttgcgagtc gaactcgatc gcgtgcggag ttcggaactc gcctggtccc
     1321 gttccggtct taccgatcat gcgcaggaac ttgccgtcac gatcgaaaac cgaaatacgg
     1381 ccgacaagct tcgggtcgtc gacgttcgta tggctctccg cgatatagat atcgccgttg
     1441 ttggggtcgg tgacgacatc gttcggctca gtgagcgcgt ccggcggatc gcccttcacg
     1501 ccgggtttgc ccaaggtcat cagtaccttg ccatccggac tgaatttcat aaccacactg
     1561 cctttctttt cctcacccgg atattgggtg cgttcttccg ccgtcggaat tcgagcatcg
     1621 gttacccaga cgtttccctc gcgatcgacg tgaattccat gcggccatac gaacgtgccg
     1681 ccgccaaaac ttctgatctc ctttcctgtc tcatcgaaga ggtggaccgg attcgccctc
     1741 gtgccgaggc aacccggcgc aattcccggc gagcaacggt cggtcgccca gacggacttg
     1801 ccgtctcgat cgatggcgac accgttggaa ccgccccacg gccttttctc caacgtgagc
     1861 tgcgcccaat cgcggacggc gcgataagga tttggtccgc tgttgacggg cggaactttg
     1921 tcggtctcgg cagtcccgcc gcgctgttgc gccttgacgc gggtgccttg cgcatcgata
     1981 cagacgacga tcgccagcac gcccgcgagt ctcacgacag ttgaggtggt catcttggtg
     2041 ctccctgctg aatgaatgca aatttatcgg cgcttttttc tgaacgagcc gatatcgcga
     2101 ttgcggcgaa ccgtcaatcg tgtaagccgc ctcaaatcgc gttctcaatc ttcttcccta
     2161 gcacctgcca tttcgagcca gtcgagttcc tcctccatta gatgaaacgc atcgtcgccg
     2221 atctcgtcgt tggcacgcat ctcgagaaca gcttggcgcg ccgcctggag cgctccacga
     2281 tagatgtcgc tatggttcga gcgcgtgata tcccgagcgt cagcctccgc tcgttcattc
     2341 gctagacgtg cggtgaactc ccgccggacg agttcggcga ccggcgatcg gtcctccgcg
     2401 aaactcccga gtcccgcacg caatgcgcgt tcacgcgccg tgttcagttc gcggccgaca
     2461 ggatcgtcgt cattgagatc gagcgcgcgc aagagcggct tgagcgtgag tccctgcact
     2521 accagcgtac cgaggaccac agagaacgca gtcagcacga tcaggtcgcg gtagggaaat
     2581 tccgccggaa gcgccatcgc tgcggccaga gatagaatcc cgcgcatccc g
//
LOCUS       DASULZ010000827         2597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1108394, whole genome shotgun sequence.
ACCESSION   DASULZ010000827 DASULZ010000000
VERSION     DASULZ010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108394"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1694
                     /locus_tag="VFY96_16995"
     CDS             <1..1694
                     /locus_tag="VFY96_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX5608226.1"
                     /translation="VASKLLGPKSGLVAGSALVVDYMLTITISIASSADALFSFLPME
                     LQPLKIFTQIVLIFALILLNLRGVKESVLFLLPIFLLFIVMHALGIVLGIGQHVANLP
                     GVVTGSYTELTRDFRSLGLVATLGILLHAYSLGGGTYTGIEAVSNGLQILREPRVDTG
                     KRTMLYMATSLAFTASGILLCYLLNQVKQEPARTLNASLFSGIFGDLFGAHSGITASL
                     VIVILLTEAALLVVAAQAGFLDGPRVLSNMSLDSWTPHRLSHLSDQLVTRNGIWFMGL
                     ASLGFLVYSHGDVRLLVVMYSINVFLTFTLSQLGMCRHWWQVRRSERAWARRFLINGV
                     GLLLTGIILFVTTTTKFTEGGWVTLLVTLLFVLGCQYIRSHYDRVNTALKSLDDTLLT
                     LPFQPDLSKPVPGQNPEGPTAALIVRDFDGISVHTLLNIQRLFPNYFKNIVFISVGVI
                     DTGQFKGHTEVEGLKKKTEEALKSFVEYANCLGWYAEYRYALGVDVITELEELCDSVA
                     KEFARVVFFSGQLVFEKENVFTRVLHNHTPYTLQQRLQFAGRDMMILPIRVFAQA"
     gene            1849..2043
                     /locus_tag="VFY96_17000"
     CDS             1849..2043
                     /locus_tag="VFY96_17000"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5608227.1"
                     /translation="MFDKEDELLNSKQVATILDMSPDTVNEFARKNILPAFKKGKQWR
                     FRRRDVSVFYSKQPKNIRDA"
     gene            2068..>2597
                     /locus_tag="VFY96_17005"
     CDS             2068..>2597
                     /locus_tag="VFY96_17005"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5608228.1"
                     /translation="MADIEAHESKLYSELAPVYDKVFGKIFYNRLERVIEDLDIPAGA
                     KVLEVGAGTGTSFPAYPTHCSITGVDLAPDMLARAQRKIEENGWSHFKLMQMDALDLK
                     FPDNTFDYVMAFHVVTVVPDPVRMIAEAKRVCKPGGRIVVVNHFTSEVPFLGAVTEAL
                     DPVTRWLGWRTDLKLKP"
BASE COUNT          542 a          707 c          708 g          640 t
ORIGIN      
        1 tggtcgcctc gaagttgctc ggcccaaaat ccggcttggt cgccgggtcc gccctggtgg
       61 tagattacat gctgaccatc acgatctcca tcgcgagcag cgccgacgcg ctgttcagct
      121 ttttgcccat ggaattgcag ccgctcaaaa tcttcaccca gatcgtgctg atcttcgcgc
      181 tgatcctttt aaaccttcgc ggcgttaagg aatcggtttt atttctgctg ccgatttttc
      241 tgttgttcat cgtcatgcac gcattgggca ttgtgctcgg catcgggcaa cacgtcgcca
      301 atttacccgg cgtggtcacc ggctcttaca ccgaattaac gcgcgacttt cgcagcttgg
      361 gccttgtcgc gaccttgggt attttgctac atgcctacag cctcggcggc ggcacctata
      421 cagggatcga agccgtcagc aatggcttac agattctgcg cgaaccgcgc gtcgacaccg
      481 gcaaacgcac gatgctttac atggcgactt ccctcgcctt taccgccagc ggcatcttgc
      541 tttgctatct gttgaaccag gtgaagcagg agcccgcccg gacattgaac gcgagcttgt
      601 tctcggggat ttttggcgat ctgttcggcg ctcattccgg gatcacggct tcattggtca
      661 ttgtcatcct cctgaccgaa gccgcattgc tggtcgtcgc cgcgcaagcc gggtttctcg
      721 atggaccgcg cgtgctctcg aacatgtcac tggattcctg gacacctcat cgactgtccc
      781 atttgagcga tcagctggtg acccgcaacg gcatttggtt catgggcttg gcttccctcg
      841 gatttctggt ctacagccat ggtgacgtgc gacttctagt cgtgatgtac agcatcaacg
      901 tctttctgac gttcacgctc tcacagctcg gcatgtgccg gcactggtgg caagtgcgcc
      961 gctctgagcg cgcctgggcg cgcaggtttt tgatcaatgg cgtcggtctt ttgctgacgg
     1021 gaatcattct ttttgtcacc accacgacga aattcactga aggcggctgg gtcactttgc
     1081 tggtcacgtt gttgtttgtc ctgggctgcc aatatatccg atcgcactac gatcgcgtga
     1141 atacggcgct caagagcctc gacgacacgc tgctcacgct gccgtttcag cccgacttga
     1201 gtaaaccggt gccgggacaa aatcccgagg ggccgacggc ggcgctgatc gtgcgcgact
     1261 tcgatgggat ctcggtgcac accctgctca atattcagcg gctcttcccg aactatttca
     1321 aaaatatcgt cttcatttcc gtgggcgtca ttgacactgg ccaattcaaa ggtcacactg
     1381 aagtagaagg actcaaaaag aaaactgagg aggcgctcaa gagttttgtc gagtacgcaa
     1441 actgcttggg gtggtatgca gaatatcgct atgccttggg agtggatgtc atcaccgaac
     1501 tggaagagct ttgtgattca gtggctaagg agtttgcgcg ggttgtgttt tttagcggcc
     1561 agctggtttt tgaaaaggaa aatgtcttca cccgcgtgct ccacaatcac accccttaca
     1621 cgctgcagca gcggctgcaa tttgccggac gcgacatgat gatattgccg atccgcgttt
     1681 tcgcccaagc ataacgctgc gctcgttcga ttgcgccgcg tattctgcac gtttgttttc
     1741 ggtctctcgc tcgacgcgca caaggcgttt cttgccttga cagcctaggg tgaagttgtt
     1801 agatagaacg aaattttcac ctggaaatgg cacaaaagcg ggggaattat gttcgacaaa
     1861 gaagatgagt tactcaacag caaacaggtc gcgacgatct tggacatgag cccggacacc
     1921 gtcaatgaat tcgcccggaa gaatatcttg cctgcattca aaaagggaaa acagtggcgt
     1981 tttcggcgac gagacgtttc ggttttctac agtaagcagc caaaaaatat tcgtgatgct
     2041 taatctcagg aggccttagt gggaaaaatg gcagatatcg aggcacacga gagcaagctc
     2101 tactcggagc tggccccggt ttacgacaaa gtcttcggca aaatctttta caatcggctg
     2161 gaacgcgtca tcgaggacct cgatataccc gcgggggcga aggttctcga agttggcgcg
     2221 ggaacgggaa cctcttttcc cgcctacccg acccactgta gtatcacggg cgtcgatctc
     2281 gcaccggata tgctggcgcg agcgcagcga aaaattgagg aaaacggctg gtcccacttc
     2341 aagctgatgc agatggacgc tctcgattta aaatttccgg ataacacttt cgattatgta
     2401 atggcgtttc atgtcgtcac ggtggtgccg gatcctgtgc gcatgatcgc cgaggcgaaa
     2461 cgggtatgta aacccggcgg cagaatcgtc gtcgtcaacc acttcaccag cgaggtccct
     2521 tttttaggcg cggtgactga ggccctcgat cccgtgactc gctggttggg atggcgcacc
     2581 gatttgaagc tcaagcc
//
LOCUS       DASULZ010000828         2396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_806111, whole genome shotgun sequence.
ACCESSION   DASULZ010000828 DASULZ010000000
VERSION     DASULZ010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806111"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VFY96_17010"
     CDS             <1..660
                     /locus_tag="VFY96_17010"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5608229.1"
                     /translation="VRPEIKSAEDFKGKRIAISRFGSSSEVNLRIALEKIGVKADQVQ
                     IIQLGGVSPRQAALLSGQVQATVLSAPQATSAIKSGMNLLVDLSDKKWGLPRYCHNCF
                     MTTRRFLQTHRGQATSFIKAVIEGLYMMKRDKNLAVRLIKKYLRLDDESASIGYDFYI
                     AQHGEGIMSLPEPRGLDFVIAEVAKQNSKAAGATPDSLRLLDPTIVDEIRKSGFIERV
                     K"
     gene            715..1809
                     /locus_tag="VFY96_17015"
     CDS             715..1809
                     /locus_tag="VFY96_17015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5608230.1"
                     /translation="MCSRLSMNRASQFEVIVASLKRRHTGVTSTVAALIPEQAKRLDI
                     TALGPKVPPQWPRIRWSDLFRHGFNPPTGKRFRIWHARRNLEALTGLILRSVLRMPLK
                     LVLTSAAQRRHSRWTHWLMRQMDVVVATSAETANYIPVPAKIIFHGVDTARYFPAEDR
                     SREWGESGLPGRFGIAIFGRVRHQKGTDLFVDAMCRLLPKFPDFTAVIIGAVTPDQIP
                     FANNLKRIIAAAGLSERIRFLGERPATEIPLWFRRMSIVVSPQRWEGFGLVPLEAMAS
                     GCAVVATRVGAAHHVILDEHTGYLVSPQDLDALVFRLENLMRDPQLVLKMGQAGRVHA
                     VNHFSIEREAAELERVYESIWRGSGTRQDG"
     gene            1974..>2396
                     /locus_tag="VFY96_17020"
     CDS             1974..>2396
                     /locus_tag="VFY96_17020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HEX5608231.1"
                     /translation="MSRNLQRGLALLYEDKDILVVNKPAGLLTIASDSEKTRTAYFIL
                     TDYVRKRHSKARNRIFIVHRLDRETSGILVFAKTIEAKLHLQQHWDTTEKKYLAVVHG
                     RMEKETEIISSYLAENNAHLVYPTSDRSKGRLARTAYRV"
BASE COUNT          550 a          651 c          665 g          530 t
ORIGIN      
        1 gttcgacctg aaatcaaaag cgccgaggat ttcaaaggca aacgaatcgc catcagccgg
       61 ttcggctcct cgtccgaggt caatctcaga atcgccttgg aaaagatcgg cgtaaaagcc
      121 gaccaggttc agattattca gttgggcggc gtatccccgc gtcaagccgc tttgctctcc
      181 gggcaagtcc aagcgacggt gctgagcgct ccgcaggcga cctccgcgat taaatcaggc
      241 atgaatcttc tggtcgattt gtctgacaaa aaatggggac tgccccgcta ctgccataat
      301 tgtttcatga cgactaggag atttctacaa acgcaccgtg gccaagcgac tagcttcatc
      361 aaagcagtta tcgaaggcct ctacatgatg aagcgggata agaatctcgc agtccgactg
      421 atcaaaaaat atttaaggtt agacgacgaa agcgcatcca tagggtatga tttctacatc
      481 gcccagcacg gcgagggaat aatgagctta ccggaaccaa gaggtctcga ctttgtcatc
      541 gccgaggttg ccaaacaaaa ttctaaagct gcgggagcga cgccggacag cctgcggctg
      601 ctcgatccga ccattgtcga tgagattaga aagagcggat ttatcgaacg ggtgaaataa
      661 ggcttcaatc tagtgaactg atcgaacaag cgatctttct tctgagttct ccttatgtgc
      721 tcacgtttat cgatgaatcg cgcctcccaa ttcgaagtca tcgtcgcgag cctaaagcgg
      781 cggcacaccg gggtgacctc gaccgtcgcg gctttgatac cagaacaggc caaacgattg
      841 gatatcaccg ctctggggcc caaggtcccg ccgcagtggc cgcgcatccg ctggagcgat
      901 ctcttcaggc acggttttaa tccgccaacg gggaaaagat tccgcatttg gcatgcgcgc
      961 cgcaatcttg aggcgctcac aggcctgatt ctccgttcgg tgctgagaat gccgctcaaa
     1021 ctcgtgctaa cgtccgcggc gcagcgccgt catagtcgtt ggacgcattg gctgatgcgg
     1081 caaatggacg ttgttgtcgc gacctcagcg gagacggcga attatattcc ggtccctgcc
     1141 aagatcatct tccacggtgt cgacacggcg cgctactttc ctgccgaaga ccgcagccgg
     1201 gaatggggcg agagcggact tcccggccgt ttcggtatcg ctattttcgg acgggtgcgt
     1261 catcaaaaag gaaccgacct gttcgttgat gcgatgtgcc gcttgctacc aaaatttccc
     1321 gacttcaccg ccgtgatcat aggcgccgtg acaccggacc aaattccatt tgcaaacaat
     1381 ttaaagcgta taatcgcggc tgccggtctt tcagagcgca tccggtttct cggtgaaagg
     1441 cccgcgactg agatccctct ctggtttagg cgcatgagca tcgtcgtctc accgcagcgc
     1501 tgggagggtt tcgggctggt gccgttggaa gcgatggcaa gcggctgtgc ggtggtggcg
     1561 actcgggtcg gcgccgctca ccacgttatc ctcgacgagc ataccggcta tctcgtgtct
     1621 cctcaggacc tcgatgcctt ggtctttcgc ctggaaaatc tgatgcgcga ccctcagctt
     1681 gtgcttaaaa tggggcaagc cggacgcgtt catgcagtca atcacttttc gatcgaacgc
     1741 gaggcggccg agttggaacg cgtttacgaa agcatttggc gcggctcggg cacccggcag
     1801 gatggttagt ttcagggtct cgatttaatt aaagaagtaa gtccatcttc ttacataatg
     1861 acagcgttgc gcggcgttat tggttaactc gaagccgggc ggtaggcgac ctttgccggt
     1921 ggcagacagg cttactcaaa ccgatggttc ctgcgatttc ccctgatcct tctgtgtcac
     1981 gaaatcttca gcgcggcctg gcgcttcttt atgaagacaa ggacattctg gtggtgaaca
     2041 aaccggccgg cttgttaacg atagcgagcg attcggagaa aacgcggacg gcttatttca
     2101 tcctgaccga ctacgtgcgc aagcgccaca gcaaagcgcg caatcgcatc ttcatcgtgc
     2161 accggctcga tcgtgaaaca tcgggaatcc tcgtctttgc caagacgatc gaggcgaagt
     2221 tgcatctgca gcagcattgg gacaccacag agaagaaata cctggcggtg gttcacggcc
     2281 gtatggaaaa agagacggag attatttcta gctatctggc agaaaacaat gcgcacctgg
     2341 tttacccgac ctctgacagg agcaaaggca gattggcccg caccgcctat cgagtg
//
LOCUS       DASULZ010000829         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_604024, whole genome shotgun sequence.
ACCESSION   DASULZ010000829 DASULZ010000000
VERSION     DASULZ010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604024"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..1097)
                     /locus_tag="VFY96_17025"
     CDS             complement(162..1097)
                     /locus_tag="VFY96_17025"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX5608232.1"
                     /translation="MSIVLNPARDIKIGTSGYSYSSAPPKGWYGAFYPQKKPKEFDEL
                     RYYAQIFNTCEINNTFYRPPAPAVTKAWSEKTPDDFSFAVKIWQKFTHPIKISRKKSD
                     ERWEPPSEAEFDEFRAGILPLAEAGKLGVLLFQYPAGFHYLPENVEKLHAALRRFYDY
                     PKVVELRHSSWNENAGAMKDLLLENRASGVLIDEPKFAGSIRQAPEAIGEIFYFRAHG
                     RNAKAWWHAKESWERYDYLYSRDEIKKHAERITTAAATPGVKKAFAFYNNHARANAAA
                     NAIMLSQELGVRLKSMPPEEMVAKFPELVQHSTTK"
     gene            complement(1194..1868)
                     /locus_tag="VFY96_17030"
     CDS             complement(1194..1868)
                     /locus_tag="VFY96_17030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5608233.1"
                     /translation="MKRTKKKSQSASTDPDSVSRLDEYNRKRRFDVTPEPAGKPRRTR
                     KKALQFVIQKHRATSLHYDFRLEHDGVMLSWAVPKGPSLDPATKRFAMQTEDHPIEYN
                     RFEGVIPEGEYGGGTVMIWDHGTWEPEVADVDAALAKGDVKFTLYGKKLRGSWVLVRM
                     RQRQWLLIKHRDQAASSLDITAVEFRSAVSDRTLAEIARRAGASARQLKQAEEADRTA
                     ASTGAA"
     gene            complement(1947..>2542)
                     /locus_tag="VFY96_17035"
     CDS             complement(1947..>2542)
                     /locus_tag="VFY96_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX5608234.1"
                     /translation="YSGVGLHIRREAFAEEPVFNHPSFDFETRVVTAQLADFLFASVY
                     VPNGGKDFTAKMQFLRQMEEFVAAARADNMRLVVCGDLNIARTERDVHPKERNPIAVG
                     QRPEEREIFERILAQGLIDVGRALDPDNDNLFTWWAPWRNMRARNIGWRLDYILASEA
                     LAANAIRCATFREIGTSDHAPVIAEFDVSQAVPANGK"
BASE COUNT          533 a          687 c          709 g          613 t
ORIGIN      
        1 agggaatgtt ttcggcaacg agcaccagat gtgaccctcg ccgcttgcgg gagagggaag
       61 ggcgagggtc cgcagagtac acgaaatttg ctctggtgtg ctccgctcac cgagtggtag
      121 cccgaacgca acgtggcgaa atcgtgtttc aaccccatcg attattttgt cgtgctgtgc
      181 tgcaccagct caggaaattt cgcaaccatt tcttccggcg gcatcgattt aagccttacg
      241 cctaattctt gagacagcat gatcgcgttt gccgcggcgt ttgcgcgcgc atggttgttg
      301 tagaaggcga aggccttttt gacgcctggc gtcgctgcgg cggttgtgat gcgctcggca
      361 tgctttttga tttcatcgcg cgagtacaaa taatcatagc gctcccagga ttctttcgcg
      421 tgccaccacg ctttggcgtt tcggccgtgc gcgcgaaaat aaaaaatctc gccgatcgct
      481 tcgggtgctt gccgtatcga gccggcaaac ttcggctcat cgatcaggac tccgctggcg
      541 cggttctcca atagtaggtc tttcattgca ccggcgtttt cattccagct actatgacga
      601 agctcgacaa ctttcgggta atcataaaac cgcctgagcg cggcgtgcag cttttctaca
      661 ttttcgggta agtaatgaaa tccagctggg tactggaaca acaaaacacc gagcttgccc
      721 gcctcggcca atggcagaat gccggcgcga aattcgtcga actcggcttc gctcgggggt
      781 tcccatcgct catccgattt cttccgagaa attttaatcg ggtgcgtaaa tttttgccat
      841 atttttacgg cgaacgaaaa gtcatccggc gtcttctctg accaagcttt cgtcacggcc
      901 ggcgcaggtg gtctgtaaaa cgtgttattg atctcgcacg tattgaagat ttgcgcgtaa
      961 tagcgaagct cgtcgaactc tttgggcttt ttctgcgggt aaaaagcccc ataccagcct
     1021 ttgggcggcg cgctcgagta ggaatagccc gaagttccaa tcttgatgtc tctggcggga
     1081 ttaagcacga tgctcatcgt cagtcattgc gggcagaagc tgtaacgagc ttgcaaacgg
     1141 tatgaagcag acgcgctgag acttttgatt ccggagcaaa tatgcggaag ctgtcaagca
     1201 gcgccggtcg atgccgcggt acgatccgcc tcctcggctt gtttgagctg gcgtgcgctc
     1261 gcgccggcgc gccgcgcaat ctcagccaag gtgcggtccg atacagcgga gcgaaactcg
     1321 acggcagtga tgtccaagct cgaagcggct tgatcgcggt gtttgatgag aagccactgg
     1381 cgctggcgca ttcgtaccag cacccaggaa ccgcggagtt tcttgccgta cagtgtgaat
     1441 ttgacatctc ctttagccag cgcagcgtcg acatccgcaa cttctggctc ccacgtgccg
     1501 tgatcccaaa tcatcacggt tccaccaccg tactctcctt cgggaattac gccttcaaaa
     1561 cgattgtatt cgatcgggtg atcttcggtt tgcatggcaa agcgtttggt ggcggggtcg
     1621 agggacggtc ctttgggaac cgcccaagag agcatgacgc cgtcatgctc aagccgaaag
     1681 tcataatgta agctggtcgc gcgatgtttc tgaatcacga attgaagggc tttttttctc
     1741 gtgcgccgag gctttccggc gggttccggc gtcacatcga agcggcgttt gcgattgtat
     1801 tcgtccagac gactgacaga gtcaggatcg gtgctcgccg actgggattt ttttttcgtc
     1861 cgtttcattt cattggagaa cagcagtcga ttgcttaatc gggttccgaa actcttttga
     1921 aaaattgcct attgaagctc aggcactcac tttccatttg ccggaacggc ttgactcaca
     1981 tcgaactctg caatgacggg ggcatggtcg ctcgtgccta tctcccggaa tgtcgcacag
     2041 cgaattgcgt tggcagccag cgcttcgctc gccagaatgt agtcgagccg ccagccaatg
     2101 ttgcgcgcgc gcatgttgcg ccagggcgcc caccaagtga atagattgtc gttgtcggga
     2161 tcgagcgcgc gccctacgtc aataagacct tgcgctagaa tacgctcaaa gatttctcgt
     2221 tcctcgggcc gttgtccgac cgcaatcgga ttgcgctcct tagggtggac gtcgcgttcg
     2281 gtgcgggcga tattgagatc gccgcaaacg acaagacgca tgttatcggc ccgagctgcc
     2341 gcgacgaact cttccatctg ccgcaaaaat tgcatcttgg cggtgaaatc cttaccgccg
     2401 ttcggtacat agaccgatgc gaacaaaaaa tcagcgagtt gcgccgtcac gacccgagtt
     2461 tcgaaatcga aactgggatg attaaaaacc ggctcttcgg caaaagcttc gcggcgaata
     2521 tgaaggccaa cgccggagta gg
//
LOCUS       DASULZ010000830         2944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_909048, whole genome shotgun sequence.
ACCESSION   DASULZ010000830 DASULZ010000000
VERSION     DASULZ010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909048"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..2583)
                     /locus_tag="VFY96_17040"
     CDS             complement(139..2583)
                     /locus_tag="VFY96_17040"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX5608235.1"
                     /translation="MDLPTGGFFSKFFSALLRSSIHFLDRGALPVRKGELHLAGLTRP
                     VEVLWDHHAVPHVSAFDEHDLFFTQGYLHAQERLWQMEMNRRFLRGRMAEIFGDFALP
                     WRELSSHFRGRTSVDFDYFVRLIGIRAAASASLAIIKEDDALRLRSYCDGVNRYIENC
                     GKRLPWEFRLLRYEPEPWQPEDTLTIGKGFAFLLSTALYTRLNFIAVAAKLADEPAKL
                     RSLFPSYPDDAPVSARSAWDAARGLWQFCSGVLAVTDWHPAGSGSNCWAIAPQRAESA
                     AAILCNDPHLRLTLPSVWYLALLRVEPDVPQSNPYEAWGASIPGVPFIQVGHNRHVAW
                     GITAALCDDAEIYREKVHPTEPNLYLAGERWQTFQTRKERIAIHGKRSVEKIIRFTRH
                     GPTISDFAELPGRGEVLTARWTAQEPTQELHSMYGINCAQTWREFLGSLRDHSAPSLN
                     FIYADREGNIGYTLAGKIPRRRETASLLPVSGWDETNDWLGYIPFEELPRVYNPPEGV
                     VANANNRITDRAYPYYLSQFFEPPHRVRRIEQLLDSRSKFNFEQMAQVQFDCLSLHGV
                     ELIARLSGELSQVPDDDPSLKAAANRLLAWDGRCDANSVAAAIFHLFHYRLLQNLLIP
                     TLGEEVCSAYTEILNQCIVPTDRILADPSSLWFSRQSRYALVVSALRETCGELKEKFG
                     SNLEAWHWGKIHQLHMTHAFGRTRVFKHPLGIGPLPTPGDGMTINLGFYRHSNPFTHT
                     VGSSLRFVAELGESIRSQIVLPSGQSGHRSSAHYSDQAGLWIKNERISLTGIPPDHRL
                     LLKPVSANIVATSSPG"
     gene            complement(2725..>2944)
                     /locus_tag="VFY96_17045"
     CDS             complement(2725..>2944)
                     /locus_tag="VFY96_17045"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEX5608236.1"
                     /translation="TAKAELKKLLQRLEDEIGEQQYLLGDFSLVDADLLPRFTRLEGF
                     GVLPDASLPRLGNYLERVKARPSVRAVL"
BASE COUNT          713 a          776 c          837 g          618 t
ORIGIN      
        1 catcggcgac cactcgaata tctgtccccg tttcgaggag aattttcagg ctctggcgga
       61 agagccgttc gtcatcggca atgagtagct ttatggacat aaatactcgt acgagtgctt
      121 ttgatttggc agtgcgtttt accccggact ggatgtcgcc acaatattgg ctgaaacagg
      181 tttgaggagc aagcgatggt ccggcggtat accggtcagc gaaatccgtt catttttgat
      241 ccatagacct gcttgatcgc tatagtgagc ggatgaccgg tgaccggact ggcctgaggg
      301 caagacgatt tgggatctga tcgattcacc cagctctgca acgaatcgca acgacgagcc
      361 gactgtatgg gtgaaggggt tcgagtgccg atagaaaccg agattgatcg tcataccatc
      421 accgggtgtc ggtaacggtc cgattcccag tgggtgcttg aaaacgcgtg tgcgaccaaa
      481 cgcgtgcgtc atatggagct gatgaatctt gccccagtgc catgcttcga ggttgctgcc
      541 gaatttttcc ttaagctcac cacaggtttc gcgtagcgca gagacgacga gtgcatagcg
      601 cgactggcgc gaaaaccaga gggagctcgg gtcggctaaa attcgatccg tgggaacgat
      661 gcactggttt aaaatttcag tataggcaga gcaaacttcc tcgccgaggg tcggtatcag
      721 aagattttgc aataggcggt aatgaaacag atgaaaaatc gcagcagcga cgctattggc
      781 atcacaacgg ccgtcccagg cgagcagtcg gttggctgcg gctttaaggc tcgggtcatc
      841 atctggaacc tgcgatagct cgccgctcaa ccgagcgatc aattcaaccc cgtgaagcga
      901 caaacagtca aactgcacct gcgccatctg ttcaaaatta aactttgagc gcgaatccag
      961 cagctgctcg atccgacgaa cccggtgagg cggttcgaaa aattgcgaaa gatagtaggg
     1021 gtaagcgcga tcagtgatgc ggttgttggc attcgcgacc accccttccg gtggattata
     1081 gacgcgcggc agttcttcga aaggaatata acccagccaa tcgttcgttt cgtcccagcc
     1141 cgagacgggg agcaacgaag ctgtttcacg tctgcgcgga attttacctg cgagggtata
     1201 accgatattc ccttcgcgat cggcatagat gaaatttaga ctgggcgctg agtgatcacg
     1261 taagctgccg agaaactcgc gccaggtttg tgcgcaattg atgccgtaca tactgtgcag
     1321 ttcctgagtc ggctcttgag cggtccatcg cgcggtcaga acttctccac ggcccggcag
     1381 ctcggcaaaa tcactgatcg tgggtccatg gcgtgtaaag cggataatct tttcaactga
     1441 gcgcttgccg tggatagcga tgcgctcctt gcgagtttga aatgtttgcc agcgctctcc
     1501 ggcaaggtaa agatttggct cggtcgggtg caccttctcg cgatatattt cagcatcatc
     1561 gcagagcgcc gctgtgattc cccacgccac atgccgatta tggccgacct gtataaatgg
     1621 aacgccggga atcgaggcac cccaagcttc gtacgggttt gactgcggga cgtcgggttc
     1681 gacccttaaa agcgccaagt accagaccga gggaagtgtc aaccgcaggt gcgggtcatt
     1741 gcatagaatg gcggccgcgc tctcagcccg ctgcggcgca attgcccaac aattactacc
     1801 gctgcctgcg gggtgccaat cagtgactgc taaaactccg ctgcaaaatt gccacaaacc
     1861 gcgagccgca tcccacgcgc tccgggcgct caccggagca tcatcgggat agcttgggaa
     1921 caagctccga agcttagcgg gttcatcggc cagttttgcc gcgacggcaa tgaaattgag
     1981 gcgggtgtag agcgcggtgg agagcagaaa cgcgaaaccc ttgccgatgg tcagtgtatc
     2041 ttccggctgc cagggttccg gctcatatcg gagcaagcga aattcccagg gcaagcgctt
     2101 accacagttc tcgatatacc ggttgacgcc atcgcagtag gaacgaaggc gaagcgcatc
     2161 gtcttctttg atgatagcga gcgaagccga ggctgcagcg cgaataccga tgagccgcac
     2221 gaaatagtca aaatcgacag aggtccgacc gcgaaaatga ctcgagagct cacgccacgg
     2281 taaggcaaag tcaccaaaaa tttctgccat gcgacccctc aaaaagcgcc gattcatttc
     2341 catttgccaa agccgctcct gcgcgtgcag gtatccctgc gtaaaaaaca ggtcgtgctc
     2401 atcgaaggcg gagacgtggg gaacggcgtg atgatcccag agaacttcga ccggccgggt
     2461 caaaccagcg aggtgcaatt cccctttacg gacaggcaac gcgccgcggt cgagaaaatg
     2521 gatggatgaa cgaagcagcg ccgaaaagaa tttggagaaa aatcctcccg tcggcaaatc
     2581 catcgaagct atggaaatga tgttgcgata tcgtgttgcg gcgaaggcga atcgatacct
     2641 cgaggattct tagttggact cagcatgagc gtcgcgaaat tttgatgcag ttttgattcg
     2701 aaatccgagc gaaagcccgt ggcgttacag aaccgcccga accgatggcc gtgcttttac
     2761 tctttcgagg taatttccca accgaggcaa cgatgcatcc ggcaacacgc cgaagccttc
     2821 caggcgcgta aagcgcggca agagatcagc gtcgaccaga gagaagtcgc ccaacaagta
     2881 ctgttgctcg ccaatctcat cctcgaggcg ctgcaagagt tttttcaatt cggctttggc
     2941 ggtc
//
LOCUS       DASULZ010000831         5867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1516865, whole genome shotgun sequence.
ACCESSION   DASULZ010000831 DASULZ010000000
VERSION     DASULZ010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5867
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516865"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="VFY96_17050"
     CDS             <1..1101
                     /locus_tag="VFY96_17050"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX5608237.1"
                     /translation="IRFSARGQMNGTRLYLSLDSGGHRSSRELKLRQAPYLLAALKPY
                     VVTQQLETGKKFLFPTFDPSTLSEQVTTVVIEGREQLRIADRTEPAIRLRQSFRGISV
                     VAWIDGQGRTLKEESPAGVSLVRQEMSAARNLPARAVSLDLIAQTAVPVATQIADPQT
                     QHALRLKLGGVNLANFRLNGGRQTLLQDRLEIRLEDLKQLSLPKIPLRDPRLASYLQP
                     TPFLQTDHPDIQGLAAQILNGERDAYRAAIKIKDWVYREIAKEPTVSIPNALEVLQTR
                     RGDCNEHTVLFNTLARAAGIPAKTVVGVVYLRGAFYYHAWSEVWLGDWVSLDSVLNQF
                     PADVTHVKFLEGEIDRQIEILQLIGNLKIEVL"
     gene            1344..2087
                     /locus_tag="VFY96_17055"
     CDS             1344..2087
                     /locus_tag="VFY96_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5608238.1"
                     /translation="MIQLIDLIKRYGELAAVNGLNLEVPAGQIFGFLGPNGAGKTTTI
                     RVMMGILKATSGRVLLDGHDVVSEAQAAKAIAGFIPDRPFIYEKLTGGEFLRFVGKLH
                     GLDRSRLEQRITELLDHLELSSWKHELVEGYSHGMKQRLVVCAALIHQPRILIVDEPM
                     VGMDPRGARTLKDLFRALAQDGVTIFLSTHSIDTAEEICHRIGIIRQGKLIACGSMAE
                     LQGQAEGNHSNLESVFLELTREQIAAPNL"
     gene            2084..3775
                     /locus_tag="VFY96_17060"
     CDS             2084..3775
                     /locus_tag="VFY96_17060"
                     /inference="COORDINATES: protein motif:HMM:NF028259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608239.1"
                     /translation="MSTVVLLLSPFWLSWKNSLSTRSSSWSRRLVLAALAACFWLSTY
                     WVIRRVLRYFETVYELGPALAYQLLLIILLTFLSMLLFSNLVASLSTFFLARDLELLH
                     AAPVSREGFFFARLIQTTVNSSWMILFFSVPIFAAYGSVFGGSWILYVWIGLMMPLFL
                     VIPAALGILITHGLVYFLPAKRIRDVLFFIGLIGFVVVYFLFRFSQPERLVEPESFGN
                     FMQFLSAMETPSSPFLPSSWSAEILAGSLFNRETAQGFSFALLASYALFLPVAASWIS
                     GVIYFDGWSKAQETRQGRRNFLWFHRFLNGLTGLFPTEIGAIMLKDIKTFMRDTTQWS
                     QLFLLLALIVVYLYNFKVLPLDRSPMPAGTLRIVVSFANLALAGFVLSAVAVRFAFPA
                     VSLEGRALWILQSAPVSLRSLVWSKFWLVFVPLLFLGELLIFLSNLLLHVPSWMMVTS
                     LLTVFLMTFGITAIGIGLGALYPNFNYENAAEIPTSFGGAIAMILSVAFISVAVTIEA
                     WPVYQLSIASLRYARVATPSASVLAPAMVAVGVLTAAVVIISLRIGIKRLEQLRE"
     gene            3777..4442
                     /locus_tag="VFY96_17065"
     CDS             3777..4442
                     /locus_tag="VFY96_17065"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX5608240.1"
                     /translation="MELIHTVYQPSGRGPFPTLLTLHGRGANAFDLLGLAPHLCGERF
                     MVICPQAPLETPIGPESVGYAWYPMSMGGPPDVDAILASRQRLEVFVDECAQSYPIDP
                     QKFAIVGFSQGGVMAYSLALANPHRFTALAVLSSWLPKELLPRIEISTAVQSLPTIVQ
                     HGSEDSTIPVDRARSSVEILRQLKVPLTYREYVMGHEIGAKSLADLSAWLEEKILSGD
                     KTT"
     gene            complement(4495..5373)
                     /gene="htpX"
                     /locus_tag="VFY96_17070"
     CDS             complement(4495..5373)
                     /gene="htpX"
                     /locus_tag="VFY96_17070"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEX5608241.1"
                     /translation="MNRLRTTILLAVLTALVVWIGQMFGGPNGAVIALVLAGVMNFFS
                     YWFSDKIVLRMYGAQEITPNDDPELYAIVRELTVKAGLPMPRVYIIPEETPNAFATGR
                     NPEHAAVAVTHGIRRILDRRELTAVLGHELSHVKHRDILVSSIAATLAGAISYLAHMA
                     QWAAIFGGGGRDREEGGGNIFGLLFMMIVAPLAAMLIQMAVSRSREYMADEGGAKVTS
                     DPLALASALRKLQMGAQNIPMQVSDATATSTAHMFIVNPLSGGGIANLFSTHPPMEER
                     IARLEAMARDMSYMRR"
     gene            5539..>5867
                     /locus_tag="VFY96_17075"
     CDS             5539..>5867
                     /locus_tag="VFY96_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5608242.1"
                     /translation="MRLGVVFPQTEIGSDPVVIKAYAQTAEALGYTHILAYDHVIGAN
                     LASRPGWQPPYSHLDTFQEPLVLYSYLAGLTKTIELTTGIIILPQRQTVLVAKQAATV
                     DVLSGGRL"
BASE COUNT         1173 a         1672 c         1625 g         1397 t
ORIGIN      
        1 atccgtttca gcgcgcgcgg tcagatgaac ggcactaggc tctacttgag cctcgactcg
       61 ggtggccatc gatcaagccg tgagctgaag ctgcgccaag ctccctatct gctcgctgcg
      121 ctaaaaccct acgtcgtgac acagcagctc gagaccggca agaaatttct tttccctact
      181 ttcgatccgt ccacgttgtc cgaacaagtc accaccgtcg tcatcgaggg ccgggagcag
      241 ctgcgcatcg ctgatcgtac cgagccggcg atccgcttgc gccagagctt tcgcggcatc
      301 tcggtagttg catggatcga tggacaaggg cggacgctga aagaagagtc acccgccgga
      361 gtttcgcttg tgcgtcaaga gatgagcgca gcaaggaatc ttccggcgcg cgcggtttca
      421 ctcgatctca tcgcccaaac ggcggttccg gtagcgacgc agatcgcaga tccgcagacc
      481 caacacgcgc tccggctaaa gctcggtggc gtcaatttgg ccaacttccg gctcaatggc
      541 gggcggcaga ctcttttgca ggatcgactg gagattcgtc tcgaagattt aaagcagctg
      601 agtctcccga aaattccgct gcgcgatcct cgcttagctt cttacttgca gcccacgccg
      661 tttctccaga ccgatcatcc ggatattcag ggcctcgctg cacagatttt aaatggcgag
      721 agggatgctt accgcgcggc aattaaaatc aaggactggg tttataggga aatcgccaaa
      781 gagcccacgg tcagtattcc caatgcgctg gaagttctcc aaactcgaag gggcgattgc
      841 aacgagcaca cggtgctctt caataccctg gcgcgcgcgg cgggaatacc ggcgaaaacc
      901 gtggtcggcg ttgtctacct gcgcggcgct ttttactatc atgcgtggtc ggaggtgtgg
      961 ctcggcgatt gggtctcgct cgattcggtg ctcaatcaat ttcccgccga cgtaacccac
     1021 gtgaagtttc tcgaaggcga gatcgatcgc cagatcgaga ttctccagct catcggcaat
     1081 ctgaaaatag aagtcttata attttagatt ttggatttta gattttcgat tatcggaaag
     1141 aactcgctag atcatccggt tttttgccgt catgtggatg gaagccggta tccagatgta
     1201 aattgaattt cctggattcc gggtcgcgct agctatcgcc agcttgcccg gaatgacaaa
     1261 tgcatttttg ttgcgtattt tgtttccaat agaaaatgga aaatcgaaat cggcaatcgg
     1321 ataatcggaa atccaaaatt gtcatgattc agctcatcga cctcattaag cgctacggcg
     1381 aattggccgc cgtgaacggg ctcaaccttg aagttcccgc gggacagata ttcggctttc
     1441 tcggtcccaa tggcgccggc aaaaccacca cgatccgggt gatgatgggc atcttgaaag
     1501 cgacttcggg acgagtgctc ctcgacggac acgacgtggt tagcgaagcg caagccgcta
     1561 aggcgatcgc cggttttatt cccgatcggc cgtttattta cgagaagctc actggcggag
     1621 aatttttgcg tttcgtcggg aagctccatg gcctagaccg aagcagactc gaacaacgta
     1681 tcaccgaact gctcgatcat ctggagctca gctcctggaa gcatgagctc gtcgagggct
     1741 actcccatgg tatgaaacag cggctggtgg tctgcgcggc attgattcac caaccgcgga
     1801 tactgatcgt cgacgaaccc atggtcggca tggacccgcg cggcgcgcgc acgctcaaag
     1861 atttgtttcg tgcgctggcg caagacggcg tgacaatttt tctttcgacc cacagcatcg
     1921 acaccgccga ggagatctgc caccggatcg gcatcatccg ccaaggcaaa ttgatcgctt
     1981 gcggcagtat ggctgagctg caaggtcaag ccgagggaaa tcacagtaac ctcgaaagcg
     2041 tttttctgga gctcacccgc gagcagattg ccgcgccgaa cttatgagca cagtggtctt
     2101 gctgctttcg ccgttttggc tctcctggaa aaattcactt tccactcgca gcagttcgtg
     2161 gtcgcgtcgg ctcgtgctcg cggcgctcgc cgcatgcttc tggctcagca cctactgggt
     2221 gattcgccgc gtgcttcgct acttcgaaac cgtctacgag ctcggccccg cactggctta
     2281 tcagctgctg ctgattattc tgttgacctt tttgtcgatg ctcttgttca gcaatctggt
     2341 ggcgtccctc tcgacttttt tcctcgcccg cgatctggag ctgctccatg cggcgccggt
     2401 gtcgcgcgag ggcttttttt tcgcccgttt gatccagacg accgtcaact catcatggat
     2461 gatcctgttt ttcagtgtgc cgatcttcgc ggcctacggc tctgttttcg gcggcagctg
     2521 gattctttat gtctggattg gcctgatgat gccgctgttt ctcgtcattc cagccgccct
     2581 gggcattctg atcacccatg gcctggtcta tttcctgccc gcaaaaagaa ttcgcgacgt
     2641 gctgtttttc atcggcttga tcggctttgt cgtggtgtat ttcctgtttc gcttctccca
     2701 acccgaacgc ctggtcgaac cggaatcctt cggcaatttc atgcagtttt taagcgcgat
     2761 ggaaacgccg tcttcgccgt ttctgccgag cagttggtcg gcggaaatcc tcgcggggag
     2821 tttgttcaac cgcgaaacgg cccagggctt ttcgtttgcg cttctggcga gctatgcgct
     2881 gttccttccg gtagcggctt cctggatttc cggcgtgatc tatttcgacg gctggtccaa
     2941 ggcgcaggaa acgagacagg gtcggcggaa ttttctctgg tttcatcgat ttctgaacgg
     3001 gctcacaggc ctgtttccga ccgagattgg cgcgatcatg ttgaaggaca tcaagacgtt
     3061 catgcgcgac acgacgcagt ggtcgcagct ttttctcctg ctcgctctga ttgttgtcta
     3121 cttgtacaac ttcaaagtct tgcccctgga ccgttcgccg atgcccgccg gaactctgcg
     3181 catcgtcgtt tcctttgcca atctcgcgct cgccgggttt gtgctgagcg ctgtcgcagt
     3241 ccgttttgcg tttcccgccg tcagcctgga aggcagagcc ttgtggattt tgcagagcgc
     3301 gccggtatcg ctacgatctc tagtctggag caagttttgg ctggtttttg tgccgctgct
     3361 tttcctcggg gaattactca tcttcttgag caatcttctg ctgcatgtgc cgtcatggat
     3421 gatggtgacc tctttgctga cggtcttttt aatgaccttc ggcatcactg ccattggcat
     3481 cggccttggc gcgctgtacc ctaacttcaa ttacgagaac gccgccgaaa tcccgacgag
     3541 cttcggcggc gctatcgcga tgattctaag cgtcgccttt atctcggttg cggtgaccat
     3601 cgaagcctgg ccggtttacc aactgagcat cgcttcgttg cgctatgcga gggttgccac
     3661 acccagcgcc agcgtgctgg cgcctgcaat ggttgctgtc ggcgtgctga ctgcggcggt
     3721 cgtaatcatt tcgcttcgga ttggtattaa acgtctagag cagttaagag agtaaaatgg
     3781 agctcattca tacggtttat cagcccagcg gtcgggggcc ctttcccact ctgctgacgc
     3841 tgcacggacg cggcgctaac gctttcgatc tcttggggct cgcgccccat ctctgcggcg
     3901 aaagattcat ggtcatttgc ccacaggcgc cgctcgaaac cccgattggg ccggagagcg
     3961 tcggctatgc ctggtatccg atgagcatgg gcgggccgcc cgatgtcgat gcgatcttgg
     4021 cgtcgcggca aaggctcgaa gtgtttgtcg atgaatgtgc gcaatcctat cccatcgatc
     4081 cgcagaaatt cgccatcgtc ggtttcagcc agggtggtgt aatggcttac agtctggcgc
     4141 tggcaaatcc ccacaggttt acggcgctcg cggtattgag cagctggctg ccaaaagagc
     4201 tgttgccgcg gattgagata agcaccgccg tgcaatcgct gccgaccatc gtgcagcacg
     4261 gcagcgaaga ttcgacgatt ccggtcgatc gcgcgagaag ctcggtggaa atactgcgtc
     4321 aattgaaggt gccgctgact taccgtgaat acgtcatggg tcacgaaatt ggcgcgaaaa
     4381 gcctagccga tttgtcagcc tggctggaag aaaaaattct atcgggtgac aaaaccacgt
     4441 aggggcgacc ggccggtcgc ccctatacga gaaaaacagc gcctgacgta gcgtctagcg
     4501 ccgcatgtag ctcatgtctc tggccatcgc ctcgagccgc gcgatgcgct cttccattgg
     4561 cggatgcgtg ctgaaaagat tggcgatccc gccgccgctc agtggattga caatgaacat
     4621 gtgggcggtc gaggtcgcgg tggcatcgct cacctgcatc ggaatattct gcgcacccat
     4681 ttggagcttg cgtagcgcac tcgccaaggc gagcgggtcg ctcgttacct tagcgccgcc
     4741 ttcatcagcc atatattcgc gcgagcgaga caccgccatc tgaatcagca tagccgcgag
     4801 cggtgcgacg atcatcatga acaagagtcc aaagatattg ccgccgccct cctcgcggtc
     4861 gcggccgccg cccccgaaga tcgcggccca ctgcgccatg tgcgcgagat aactgatggc
     4921 gccggcgagg gtagcggcga tactgctgac gagaatatcg cgatgcttga cgtggctgag
     4981 ctcatgaccc aaaactgcgg tgagctcgcg cctgtcgaga atccgccgaa tcccatgagt
     5041 caccgcaacc gcggcatgtt ccggattacg tccggtggca aacgcattcg gcgtttcctc
     5101 tggaatgata tagacccgcg gcatcggcaa accggctttg accgtgagct cgcgcacgat
     5161 cgcgtagagc tcaggatcat cgttgggcgt aatctcttgg gcgccataca tcctgagcac
     5221 gatcttgtcg ctaaaccagt agctgaagaa gttcatcacg ccggcgagaa ccagcgcgat
     5281 cacggcgccg tttggcccgc cgaacatctg tccgatccag accacgagcg cggttaacac
     5341 cgccagaaga attgttgtac gtaaacggtt cattatcgcc tccgttaccc ggccatccta
     5401 ggtagcataa agtttaagtc aagctttctt cgccgtcgta taggtatata gtttgaaatg
     5461 tgcgtacgat ctgggcgaaa agcaagtgtc cgataatcaa ttgaaaacgt tgagagttat
     5521 ctctaaaagg aggcttaaat gcgcctaggc gtagtattcc cacaaaccga aatcggcagt
     5581 gaccccgttg tcatcaaggc ttatgcccag acggcagaag ccctcggcta tacccacatt
     5641 cttgcctacg atcacgtaat tggcgccaat ctcgccagcc gtccgggctg gcagccgccc
     5701 tacagtcact tagacacctt tcaagagccc ttggttctgt atagctatct cgccggactc
     5761 actaagacca tcgagctcac caccgggatt atcattctgc cgcagcggca aacagttctg
     5821 gttgcaaagc aggcggcgac cgtcgacgtt cttagcggcg gtcggct
//
LOCUS       DASULZ010000832         8739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_399615, whole genome shotgun sequence.
ACCESSION   DASULZ010000832 DASULZ010000000
VERSION     DASULZ010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8739
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399615"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1408)
                     /locus_tag="VFY96_17080"
     CDS             complement(<1..1408)
                     /locus_tag="VFY96_17080"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX5608243.1"
                     /translation="MRVAVDIGGTFTDVVVFDEANGSLSLAKTLSTPMELARGVLEGL
                     HKARVRLADVATLIHGSTVVVNAIIERNGAKTALVTTKGFRDVYEIGRINRPESFNPR
                     FRKHRPLVPREDIFEVTERRLADGSVRTPFDESEAREVARIIKDEGFEAVAVLFLHSY
                     RAPEHEIRMCEILRDTEPKLFLSASHELSREYREYERTSTVAANAYVGPKVSQYLGDL
                     ERRLHDQKFSGNLMIMQSNGGLSEIEMARRQCIQMMESGPAGGVVGTMALCELLDLDA
                     AIAFDMGGTTAKACVIRRGEPSLSPDYFIGGYNEGLAIRIPVLDIVEVGTGGGSIAWL
                     DEGEGLHVGPRSAGAEPGPASYGRGGTEPTVTDANVILGRLSPERFLGGEMRLDQAAA
                     ETVLHERLARPLNVSQQRAAEGMLQVATSAMANAVRHVTLERGLDPRDFTLVAYGGGG
                     PLHAASVAKELSIGKIMIP"
     gene            complement(1546..1902)
                     /locus_tag="VFY96_17085"
     CDS             complement(1546..1902)
                     /locus_tag="VFY96_17085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608244.1"
                     /translation="MSREPIIDQAKNARQTILEILRSRNHRARFMSEVYAALGRAQIK
                     REQADGALRQLEAEGAVMIREHFCADPHLAGVDLRVAALVDARGESDPQLSTIRAIDE
                     AWNQWLSEYLANHRCG"
     gene            2020..3438
                     /gene="selA"
                     /locus_tag="VFY96_17090"
     CDS             2020..3438
                     /gene="selA"
                     /locus_tag="VFY96_17090"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEX5608245.1"
                     /translation="MADQTATLLRELPSVDRLLKHSGCERLLTRYHREYITAQCRTVL
                     DKIRNEVRRGAAVDCSEAAVIARVENTIFIDSEPGHKPVINATGTILHTNLGRALLPR
                     AAIDAILAVADRPINLEYDLAAGKRGKREETLQNLLVELTGAEAATVVNNNAAAVLLG
                     LNTIAEGKEVIVSRGELIEIGGAFRIPEIMAKSGAVLREVGATNRTHPADYENAINEK
                     TALLLKVHTSNYKVVGFTAEVSLEQLVALGKKHRLPVMEDLGSGAFVDLSNYGLPKEP
                     VVAERIQTGADVVTFSGDKILGGPQAGLIVGKRDLIARINRNHLQRALRCGKLTLAAL
                     EATLRRYRQSPNVVEEIPTLRAFTRSLEEIRKMSEVLLPELQKALGAEFEIRVEDSTA
                     QIGSGALPTEELPTLVVSIEHQKRSANSIAQKFRAANPPIIGRINNDRFLLDLRTIFD
                     ANDLIPHFRANDPTSENPKSQI"
     gene            3447..4583
                     /gene="selB"
                     /locus_tag="VFY96_17095"
     CDS             3447..4583
                     /gene="selB"
                     /locus_tag="VFY96_17095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEX5608246.1"
                     /translation="MPYIIGTAGHIDHGKTSLIKALTGTDTDRLKEEKERGISIDLGF
                     AHLDLPDGTSAGIVDVPGHERFIKNMLAGAHGIDLVLFTVAADDGVMPQTEEHFDIVH
                     LLGVKMAIFVITKADLVPPSRIGEVEEEIDILTVGTDLENSPKLAVSSVTGQGLEELK
                     QLISKTLKASTQTAPTGYFRLPVDRAFVLQGHGVVVTGTALSGEVAVGAQVRCLPGNH
                     LFRVRSLQVHGKSVDRGGWGQRVAMNLTGPERASIERGQVICHEKLSLTSDRFDAFLE
                     VRPAAAKGIKNHQRLRIHMGAAERLAKIILLGGKEKLDAKASAYCQITLNEPLLVMRG
                     DHFVARDETAQRTLGGGMVIHPHANRHRKNEPELLSRLEILHKG"
     gene            4596..5381
                     /locus_tag="VFY96_17100"
     CDS             4596..5381
                     /locus_tag="VFY96_17100"
                     /inference="COORDINATES: protein motif:HMM:NF020671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HEX5608247.1"
                     /translation="MENFLAEDTAVALPIESIYQFINLREEEARARIAGLNSLRTLNA
                     EGETLHTTEGKWNQLRERILKLLKAFHSSHPLIPGMDMEELRGKLPDELAPKVFRAVI
                     ELFLGEKSIVREENLLRLATHRVQLGGQEKVLMDKIKQILGAQPLAPPDLKELEKQAG
                     VSRQRLTEVIRLLERDGSVVRVTTDMYFLASSIEQLRATLKKYLTEKGEMTAASFRDL
                     INSSRKYTIPLLEYFDRDGLTIRIGDIRRLKSSPAHDGARTTR"
     gene            5416..6471
                     /gene="selD"
                     /locus_tag="VFY96_17105"
     CDS             5416..6471
                     /gene="selD"
                     /locus_tag="VFY96_17105"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:5473..5475,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEX5608248.1"
                     /translation="MLLKNKKPVRLTQEVKAAGUAAKLGPADLAQVLHELPKFRDPDL
                     LVGTETADDAGVYRLRPDLAIVNTVDFFTPIVDDPYIFGQISATNSLSDVYAMGGEPK
                     TCMNIVCFPTGKMDIEILGEILKGGAEKVKESGAVVVGGHSIIDEEIKFGMAVTGVIH
                     PDKILRNVGVQEGDVLILTKPLGTGIITTALKKGKASEESVNEAVQSMTTLNAAAAQV
                     ARQHPVHACSDITGFGILGHALEMASGSAVTLVIESAKMPLLRGTPRLAEKGYITGGC
                     KRNKDYLNDKMLIDKNIREGLVEAALDPQTSGGLLFAIARRHAAKLLEELHAAGVSHA
                     TEVGYATSLQKSWVRLV"
     gene            6551..8647
                     /gene="malQ"
                     /locus_tag="VFY96_17110"
     CDS             6551..8647
                     /gene="malQ"
                     /locus_tag="VFY96_17110"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX5608249.1"
                     /translation="MPVGNPLQKLARLYNLQTVYRDGFAQLRAAPAEAVLAVLKSLDA
                     PISSYEDVASALRQRREELWQRRIEPVLVAWECAPLHFKLRMPVHLAEVRRKYQIALE
                     TGATVEGECSDGQRVSTREVEGIQYVAQTFTIPPLPVGYHRLELRLGDSSVESLVISA
                     PAQAYGFSSKKSWGVFCPLYALNSDRSWGAGDYTDLTAFADFVGDLQGTAVATLPMLA
                     SFLDEPFNPSPYAPVSRLFWNEFYLDVERISEFEQCASAQALVNSAAFRGELSGVQRE
                     SLVDYRRCMALKRQVIEKLLGCLQSKDSRRRRSFKRYATTHATARDYAAFRAVVERYR
                     TVWFQWPDTARAGTLSPRDYDEQAYQYHLYVQWQCEEQNRRLRASAKKRGVFLYLDFP
                     LGVNRDSYDVWRERNLFALNASGGAPPDALFLKGQNWGFPPFNPDAMRHQHYRYYIDC
                     LRHHMNVAGMLRIDHIMGLHRAFWVPAGFSAAEGLYVHHPGDEYYAILSLESHRHHVQ
                     IVGENLGTVPVYVNEAMARRNIFGMHVGVFGVNTSGERALDNVAANTVASLNTHDTTP
                     FMGFWTGADIEDRFQLGLLTETQAEQEQQFRAAQRQALLEFLENRGFLPKANGTPDAV
                     LKAWLAFLAQNEAAFLLINLEDLWLEPAPQNTPGTWQERPNWQRKARLSLEAIRHSES
                     ILQLLQTISDIRRRVG"
BASE COUNT         1938 a         2585 c         2421 g         1795 t
ORIGIN      
        1 tcggaatcat gatcttcccg atcgataatt cctttgccac ggacgcggcg tgaagaggac
       61 cgcctccgcc gtaagccaca agagtaaaat cgcgcggatc gagaccgcgc tcgagcgtaa
      121 catgcctgac cgcattggcc attgccgatg tggcgacctg gagcattcct tcggccgcgc
      181 gttgctggct tacgttcaga ggtcgcgcca aacgctcgtg caaaacggtt tccgcagccg
      241 cctgatcgag ccgcatttca ccaccgagaa agcgctcagg agaaagccgg ccgagaatca
      301 cattcgcgtc agtgaccgtc ggctcggtgc cgccgcggcc gtagctcgct ggaccaggtt
      361 ccgctccggc gctccgcggt cccacgtgaa gcccctcgcc ctcatcaagc cacgcgatgc
      421 tgccgccgcc ggtcccgacc tcgacgatgt cgagcacggg aatgcgaatc gcgagacctt
      481 cattgtagcc gccgataaag tagtccggcg acaagctcgg ctcgccgcgg cgaatcacgc
      541 aggctttggc ggtggtgcca cccatgtcga acgcgatcgc cgcatcgaga tcgagtagtt
      601 cgcaaagcgc catggtaccg acgacaccgc cggcgggccc cgactccatc atctgaatgc
      661 attggcgacg cgccatttcg atttcggaca aaccgccatt cgactgcatg atcatcagat
      721 tgccgctaaa tttctgatcg tgaagccgcc gctccagatc gccgagatat tggctcactt
      781 tcggaccgac gtaggcattc gcggccaccg tggacgtgcg ctcatactcg cgatactcgc
      841 gcgaaagctc atgagatgcg ctgaggaaaa gcttaggctc ggtgtcgcgg agaatttcac
      901 acatgcggat ttcatgctcg ggcgcgcggt aggaatggag aaacagcacg gccactgctt
      961 caaatccttc gtctttgatg atccgcgcta cttcgcgggc ttcgctctcg tcgaagggcg
     1021 tgcgcaccga gccatccgcg agcctgcgtt cggtcacctc gaaaatatct tctcgcggca
     1081 ccagcggccg gtgcttgcgg aagcggggat tgaaagattc cggccgattg atgcggccaa
     1141 tttcgtagac atcgcgaaat cctttggtgg tcaccagggc ggtcttggca ccgttccgtt
     1201 cgataatcgc gttgaccacc accgtcgagc catgaatcaa tgtcgcaacg tcggcgagcc
     1261 gcacccgagc tttgtgcagc ccttccaaaa cgccacgagc caactccatc ggcgtcgaca
     1321 gcgtttttgc cagcgacagt gagccgttcg cctcatcgaa cacaaccacg tcagtgaaag
     1381 taccgccaat gtcgaccgct acgcgcaaat tcgtttcctt cccttgtaag aacgtccacc
     1441 aatgcttggt tcaaaacgtt acaatcgttc aacccgttca aaacgtcaga tccgaaccgc
     1501 taaaacgatt tgaactgctt gaacggtttg aactgatccc gatcgctagc cacatcgatg
     1561 attcgccaga tactcgctca gccattgatt ccaggcttca tcgatcgcgc gaatcgtgct
     1621 caattgcgga tccgactcac cgcgagcgtc caccagcgcc gccactcgca aatcgacgcc
     1681 ggccaggtgg ggatcagcgc agaaatgttc ccgaatcatc actgcgccct ctgcctccag
     1741 ttgccgaagc gcgccgtcag cttgttctcg tttgatctgc gcgcgcccta acgcggcata
     1801 aacttcgctc atgaagcgcg ccctgtgatt gcggctgcgc agtatttcga ggatcgtctg
     1861 ccgcgcgttt ttggcttgat cgatgattgg ctcgcgggac attgctggtg tgtgacggaa
     1921 actctatgga agctccgcgt ccttgtcaaa tcttcgtcgg cgtttgcgga taaacctgtg
     1981 ttgaaatcgc ctgatcgctg tgtaaatctg agtttcccga tggccgacca gaccgccacg
     2041 ctcctccgag aacttccttc cgtcgatcgt ctgcttaaac attccggctg cgagcgactg
     2101 ctgacccgct accatcgcga atacatcact gcgcagtgcc ggacggtgct cgacaaaatt
     2161 cgtaatgagg tcaggcgcgg cgccgctgtc gattgcagcg aagctgcggt catcgcacgc
     2221 gtcgaaaaca cgattttcat cgatagcgag cccggccaca agccggtcat caacgcgacg
     2281 ggaaccattc tgcataccaa cttggggcgc gcgttgcttc ctcgcgcggc gattgatgca
     2341 atcctagccg tcgccgatcg tccgatcaat ctggagtacg acctcgccgc cggcaaacgc
     2401 ggcaagcgcg aagagactct gcaaaacctg cttgtcgagc ttaccggtgc tgaagctgcc
     2461 acggtggtga ataacaacgc cgctgccgtg ctgctggggt tgaataccat cgcggaaggc
     2521 aaagaagtga tcgtatcgcg cggcgagctg atcgaaatcg gcggcgcctt tcgaattccg
     2581 gaaatcatgg cgaagagcgg cgcggtgttg cgcgaggtcg gcgcgaccaa tcgtacccat
     2641 ccagccgact acgaaaacgc catcaatgag aaaaccgcgc tgttactgaa agtccatacg
     2701 agcaactaca aagttgtcgg ctttaccgcc gaagtcagcc tagagcagct cgtcgccctc
     2761 ggcaagaaac accgtctgcc ggtcatggag gatctcggca gcggcgcctt tgtcgatctc
     2821 agcaactatg gtctgcccaa ggaacccgtc gtagcggaaa gaatccagac gggtgccgac
     2881 gtcgtgacat ttagcggcga taaaatcctc ggcggtccgc aagcgggatt gatcgtcggc
     2941 aaaagagatt tgatcgccag aatcaatcgc aatcatctgc aacgcgcgct ccgttgcggc
     3001 aagctgacgt tggcggcgct cgaagcgacg ctgcgtcgtt atcggcagtc gcccaacgtc
     3061 gttgaagaga ttccgacgct cagggctttc acccgatctt tggaagagat tcgcaagatg
     3121 agcgaagtgt tattgcctga gcttcaaaaa gccttgggtg ctgaattcga aattcgggtc
     3181 gaagactcga ccgcacaaat cggcagcggc gcccttccca ccgaagaact gccgaccttg
     3241 gtcgtcagca tcgaacacca aaaaaggagc gccaactcga ttgcgcaaaa attccgcgcc
     3301 gccaatccgc cgattatcgg ccggattaac aacgaccgat ttctgcttga cctacgaaca
     3361 atctttgacg cgaacgatct catcccgcac tttagggcga acgatccgac aagcgaaaat
     3421 ccaaaatccc aaatctaaaa tcactcatgc catacatcat cggcaccgcc ggccacatcg
     3481 atcacggcaa gacgagcctg atcaaggcgc tcaccggcac cgacactgat cgactgaagg
     3541 aagagaagga gcgcggcatc tccatcgatt tgggatttgc ccacctcgac cttccggacg
     3601 gcacttccgc gggcatcgtt gacgttccgg gacacgagcg tttcatcaag aacatgctcg
     3661 ccggtgccca cggcatcgat ctcgtccttt tcacggtggc cgccgatgac ggggtcatgc
     3721 cgcaaaccga ggaacacttt gatatcgtcc atttactcgg cgtcaaaatg gcgattttcg
     3781 tgatcaccaa agcggatctg gtgccgccgt cgcgcatcgg cgaggtggaa gaagaaatcg
     3841 acatactgac ggtcggcaca gatctggaaa attcgccgaa gcttgcggtg tcctccgtca
     3901 ccggccaggg tctcgaagag ctgaagcagt taatctcgaa aacattgaag gcctcgaccc
     3961 aaaccgctcc caccggctac tttcgtttgc ccgtggaccg cgcttttgtc ctgcagggcc
     4021 atggcgtagt cgtcaccggc accgcgctga gcggtgaggt tgctgtcggc gcgcaagtgc
     4081 gctgtttacc aggcaatcat ctcttccgtg tgcgcagcct tcaggttcac ggaaaatccg
     4141 tcgacagagg cggttggggt cagcgggttg cgatgaatct cacgggcccc gagcgcgctt
     4201 cgattgagcg cggccaggtg atctgccatg aaaagctgtc actgacgtcg gatcgcttcg
     4261 atgcgtttct cgaggtccgg ccggctgccg ccaaaggcat caagaatcac cagcggctgc
     4321 gcattcacat gggcgccgct gaacgtttag ccaaaatcat cttgctcggc ggcaaagaaa
     4381 agctcgacgc gaaagcatct gcctactgcc agattacctt gaacgaaccg ctgctcgtaa
     4441 tgcgcggcga tcattttgtc gcccgcgacg aaactgcgca acggaccctc gggggcggca
     4501 tggtcattca cccgcacgcc aatcgtcata ggaagaacga gccagagttg ctgagccgac
     4561 tcgagattct gcacaaaggc tagctcgctc aggttgtgga aaattttctc gctgaagata
     4621 cggcagtcgc cttacccatc gaatcgattt atcaattcat caatctccgt gaggaagaag
     4681 ctcgcgccag gatcgcgggc cttaacagcc tgcgcaccct caatgccgaa ggcgagacgc
     4741 ttcataccac cgagggaaaa tggaatcagc tgagggaacg gatactgaaa ttgctaaaag
     4801 ctttccactc gagtcatccg cttattcctg ggatggacat ggaagagctc cgtggcaaac
     4861 ttccggacga gcttgcgccg aaggtctttc gcgccgtcat tgaactcttc ctgggagaga
     4921 agagcattgt tcgggaagaa aatttgttac gcttggcaac gcatcgggtt cagcttggcg
     4981 gccaggaaaa ggttctgatg gacaagatca agcaaatcct cggcgcccag ccactggcac
     5041 cgccggatct aaaagagctt gaaaaacagg ctggagtgtc gcggcagcgc ttgaccgaag
     5101 tcattcgcct tttggaacgc gacggctccg tggtccgggt gaccaccgat atgtactttc
     5161 tcgcgagcag catcgagcaa ttgcgtgcga ccctaaaaaa gtatttgacc gaaaagggcg
     5221 aaatgaccgc cgcttcgttt cgtgatctga tcaattccag ccgtaaatac acgattccgc
     5281 ttttggagta cttcgatcgc gatggcctta ccatccggat cggcgacatt cgccgcctga
     5341 aatcttcgcc ggcgcacgac ggagcacgca caacacgttg aaaaacgtcg gctatcgatc
     5401 tacagttggc gcgttatgct gctcaagaac aaaaagccag tgcgtctgac gcaagaggtc
     5461 aaggccgcgg gttgagccgc taagctcggc cccgccgacc tcgcgcaagt tttgcacgag
     5521 ctacccaagt tcagggaccc cgatctgctt gtcggcaccg agaccgccga cgacgcgggt
     5581 gtgtatcgac tccgacccga tctggcgatc gtcaacaccg tagatttttt taccccgatc
     5641 gtcgatgatc cctatatctt tggccaaatt tccgcgacca attccttgag cgatgtctac
     5701 gccatgggcg gtgagcccaa gacctgcatg aacatcgttt gctttcctac aggcaagatg
     5761 gacattgaaa ttttgggcga gattctcaag ggcggcgcgg aaaaggtgaa agagagcggc
     5821 gcggtcgtgg tcggcggcca cagcatcatt gatgaagaaa tcaaatttgg gatggccgtt
     5881 accggcgtga ttcaccctga caagatcctg cgcaatgtcg gtgttcaaga gggcgacgtg
     5941 ctcattctca cgaaaccgct cggcacggga attatcacga cagcgcttaa aaagggcaag
     6001 gcatcggagg aaagcgtcaa cgaagcggtt caatcgatga cgacgctcaa cgccgcggcc
     6061 gcgcaagtcg cgcgtcaaca ccctgttcac gcctgctccg acatcaccgg ctttggcatt
     6121 ctcggccatg cgctggaaat ggcgagcggc agcgctgtca ccctggtgat cgagtcggcc
     6181 aaaatgccgt tgctgcgcgg aacgccgcgg ctggccgaaa aaggctacat caccggtggc
     6241 tgcaagcgga ataaagacta tttgaacgac aaaatgctca tcgataaaaa tatccgcgaa
     6301 ggcctcgtcg aagcggcgct cgacccgcag acatcgggtg gattgctctt cgccatcgcc
     6361 agacgccacg cggcgaaact gctcgaggag ctccatgccg caggcgtttc tcacgccacc
     6421 gaagtcggtt acgccacttc tttgcaaaaa tcctgggtac ggttggtata gctatcgcgc
     6481 cgacccggcg cggtagaatt ttttcttcgg gcgagtaatc ggcgcaagcc tttgttaagc
     6541 tgtcaaaaaa atgccggttg gcaatccgct tcaaaaactc gctcgacttt acaatttgca
     6601 aaccgtttac cgcgacgggt tcgcacaatt gcgcgccgct cccgcggaag cggtgcttgc
     6661 ggtccttaaa tccctcgatg ctccgatcag ctcatacgag gacgttgcga gcgcgcttcg
     6721 gcagcgacgc gaggaactct ggcagcgccg aatcgagccg gtgctcgtcg cctgggaatg
     6781 cgcgccgcta catttcaaat tacgcatgcc agtacacctg gccgaggtta gaagaaaata
     6841 tcaaatcgct cttgaaaccg gtgcgaccgt tgaaggcgag tgtagcgatg gtcagcgcgt
     6901 ttccacgcgc gaggtcgaag gaattcaata tgtcgcgcag actttcacga ttccaccgct
     6961 tcctgtggga tatcaccgcc tcgagcttcg actcggcgac tcgagtgtgg agtccctcgt
     7021 gatttcagcc cccgctcaag cttacggttt ctcaagcaaa aaaagttggg gcgtcttctg
     7081 tccgctttac gccttgaatt cggaccggag ctggggcgcc ggcgattata cagatctcac
     7141 ggcattcgcg gacttcgtcg gcgatttgca gggaactgcg gtcgccactc tgccaatgct
     7201 cgcaagcttt ctggacgagc ctttcaatcc gagtccctat gcgcccgtga gccggctctt
     7261 ttggaacgag ttttatctcg acgtcgagcg catctccgag ttcgagcagt gcgcttccgc
     7321 ccaagcgcta gtcaactccg ccgcgtttcg cggcgaattg agcggggtcc agcgcgaatc
     7381 cttagtcgat tatcgtcggt gcatggcgct caagcgccag gtcatcgaga agctgctagg
     7441 ctgccttcaa tcgaaagatt cccgacgccg aagaagtttt aagcgttacg caacgactca
     7501 tgcgacggcg cgagattacg ccgcctttcg cgccgtggtc gagcgctacc gtacggtctg
     7561 gtttcaatgg ccggataccg cgcgcgctgg aaccttgagc ccccgcgact acgacgaaca
     7621 agcctatcag taccatctct atgttcagtg gcagtgcgag gagcaaaatc gccggctacg
     7681 cgccagcgca aaaaaacgcg gcgtgtttct ctatctcgac tttccgctgg gcgtcaatcg
     7741 cgacagctat gacgtatggc gcgagcgaaa cctattcgca ttgaatgcca gcggcggcgc
     7801 gccgccggac gcgttgtttc tcaaggggca aaactggggt ttcccaccgt tcaatccaga
     7861 tgccatgcgc caccagcact atcgttacta tatcgattgc ctgcgccatc acatgaatgt
     7921 ggcgggaatg ctgcgcatcg atcacatcat gggtttgcat cgcgcgttct gggtgcctgc
     7981 cggattcagt gctgccgaag gtctatacgt gcatcacccg ggcgatgaat actacgcgat
     8041 cttgagcctc gaatcgcacc gccatcacgt ccaaatcgtc ggcgaaaatc tcggcaccgt
     8101 gcccgtgtat gtgaacgagg caatggcgcg acggaatatc ttcggcatgc acgtgggtgt
     8161 tttcggcgtg aatacctccg gcgagcgggc gctggacaat gttgcggcga ataccgtagc
     8221 gagtcttaat acgcacgaca ccacgccatt catgggcttt tggactggag cagatattga
     8281 agatcgattt cagctgggtt tactgacaga aacccaagcc gagcaggagc agcaatttcg
     8341 ggcggcccag cgccaagcac tgcttgaatt tctcgaaaac cgcggcttcc tgcctaaggc
     8401 aaatgggact cctgatgccg tgcttaaggc atggctagca tttctcgctc aaaatgaagc
     8461 agcctttttg ttgattaacc tggaagatct ttggcttgag ccggcgccgc aaaatacccc
     8521 cgggacgtgg caagagcgac ccaactggca gcgcaaagcg cgtttatccc tcgaggcgat
     8581 ccgccactcg gagagtatct tgcaactgct gcaaaccatc agcgacattc gccgccgagt
     8641 cggctaaaat tgctaaaagc aatgggcttc aatcaagcgg cgctgattgt ccccaaccaa
     8701 ggcggtccgg gaactgacat tgcacaatcg tgctgtttt
//
LOCUS       DASULZ010000833         3707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_97288, whole genome shotgun sequence.
ACCESSION   DASULZ010000833 DASULZ010000000
VERSION     DASULZ010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97288"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VFY96_17115"
     CDS             complement(<1..570)
                     /locus_tag="VFY96_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HEX5608250.1"
                     /translation="MLTSAALVLMMTIPGLFLFYGGLVRRKNVLGTIMHSFIIVAVIS
                     LQWVLWGYSLAFGPDIGGLIGGSDWFGLNSVGPNPNPDYAATIPHAAFMVYQMMFAVI
                     TPALITGAFAERVKFSAFLVFIVLWATFIYDPLAHWVWGVKGWMREMGALDFAGGTVV
                     HISSGASALVAAFLFGKRIGYGVEAMPPHN"
     gene            complement(1033..2394)
                     /locus_tag="VFY96_17120"
     CDS             complement(1033..2394)
                     /locus_tag="VFY96_17120"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX5608251.1"
                     /translation="MNPTRRLARFALELKYQDIPAEVLDRAKACVLDTLAVCLYGSTK
                     PWSHTVIGFVHDSGLRGRSTVLGTKFSRQPAQATLANGVMAHAFELDNVRQPGAGVHP
                     GATAFVPALAMAENKRADGKSLLSAFVAGCEVMSRIGIAAGNSLERRGFHAPGLTGTF
                     GAAVAAGWLLRLDEKKMANALGIAASYSGGLIEFSRCREGAMVKRLHLGKAAEGGVTA
                     ALLASKGFAGPESVLEGKFGFCQTYSDSPQLDSLTYRLGREFESMNICIKRCACHINA
                     HAPIEALQQLREQFNFRPGDIREILVGGIEKLVTHHAIYRPTDLMMAQYSIPFCIALS
                     LYSDPTDPQSFNETQLKNKNILRIMRKVHLQVDQEIEQRGWDRAARVTVVLNNKERHS
                     ALVIHFKGTPDNPLSRSEVENKARKLTTALLSPRRLARLVETVDHLDKLKDVSQLGAL
                     LKI"
     gene            complement(2582..2989)
                     /locus_tag="VFY96_17125"
     CDS             complement(2582..2989)
                     /locus_tag="VFY96_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5608252.1"
                     /translation="MPRIVHIALKVDDLEKATEFYEKGFGFVEMETGQVRDHTSRHLT
                     DGTIDLALIKYDSEDSAEARASGLGPCIHHFAIEVDDMVKSAADLEKFGCEIISDPGV
                     VPIKFRAPGGTVAEIVPTARYKSPRPKPSRYKR"
     gene            complement(3041..>3707)
                     /gene="ispH"
                     /locus_tag="VFY96_17130"
     CDS             complement(3041..>3707)
                     /gene="ispH"
                     /locus_tag="VFY96_17130"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX5608253.1"
                     /translation="LAIIVDATCPLVEKVHREVRKFAAMGYSIILVGHENHDEIVGTR
                     GEAPENIQIVCSAAEAETIRVAHPDRVVVLTQTTLSVDDTREIVEILRRRFPRLITAA
                     KEDICYATQNRQDAVKQLAQRVDLVLVIGSRNSENSNQLCNVVRDQGKPAYLIDDHHA
                     IDPAWLEGVMRVGITSGASVPEYLVQQAAEYFRQQGAQVTQNGFIEENIHFALPVEIA
                     GAA"
BASE COUNT          849 a         1048 c         1008 g          802 t
ORIGIN      
        1 attatgcggc ggcatcgctt cgacaccgta gccgattcgc ttgccgaaca agaatgctgc
       61 caccaaggcc gaggctcccg agctaatatg caccaccgtg ccgccggcaa aatccagcgc
      121 gcccatttcg cgcatccatc ccttgacgcc ccaaacccag tgggcgagag gatcataaat
      181 gaacgtggcc cagagcacga tgaaaacgag aaacgcgctg aactttaccc gctctgcgaa
      241 cgcgccggtg atcaacgccg gagtgatcac agcgaacatc atttggtaaa ccatgaaggc
      301 cgcatgcggt atggtggcag cgtagtccgg gttggggttt ggtccgacgc tgttgagacc
      361 gaaccagtcg ctgccgccga tcaagccccc gatgtccgga ccgaaagcca agctgtatcc
      421 ccagagcacc cactgaagac tgatgacggc aacaatgatg aaactatgca tgatggtgcc
      481 aagaacattt ttgcgccgca ccaagccgcc gtagaagaga aataagccgg gaatcgtcat
      541 catcagcacg agagccgcgg aagtgagcat ccatgccgta tcgcctttgt cgattttcgg
      601 tagaggcgca gaagcagcgg gaacgggtac cgcaggagcg ccaggtgtcg tcactaccgg
      661 ccctcccggc gctgtttgcg tgacgtcttg agcacgcgtt aacgatgctg atacgcagag
      721 cgccaatact acaaagagta tcgctaccaa gttcttgcca tggtacacca cgactattcc
      781 ccctttttat ttgcgcaaga attgagcaaa caagctgact ggaatcgatt tgcgggtgct
      841 taaccgcaac cactatgcca gcaacggcac ggacagatgc gtcggcgatt cactgagtta
      901 taagatcgag cgagatattt tccggaagag cacgcttaaa aactaggcat atgcttattt
      961 ctacagcaga agtctctggc agttgtatag tcggtagagg cgcgaaaagc gtcccttgcg
     1021 gcaatcttca tctcagattt tgagcaaagc gccgagttgt gaaacgtcct tgagcttgtc
     1081 aagatggtca acggtctcaa ccaagcgggc aagccgacgt ggagataaaa gcgcggtggt
     1141 cagtttgcgc gctttgtttt ccacctctga gcggctcaag ggattatccg gggtcccctt
     1201 aaaatgaatc accaacgccg aatggcgttc cttattattc agcacaacgg tcacgcgcgc
     1261 tgcgcggtcc caaccgcgct gttcgatttc ctgatccact tgcaagtgaa ccttgcgcat
     1321 gatgcggagg atgtttttgt tcttcagctg tgtttcattg aacgactggg gatcggtggg
     1381 atccgaataa agactcagcg cgatgcagaa cggaatactg tactgcgcca tcatcagatc
     1441 cgttggtcga taaatcgcat ggtgggtcac aagcttttca atgccaccga caaggatttc
     1501 ccgaatatct cccggccgaa aattgaattg ctcacgcagc tgctgcagcg cttcaatcgg
     1561 cgcatgggcg ttgatatggc aagcgcagcg cttgatgcag atgttcatgc tttcgaactc
     1621 gcgtcccaag cgataggtga gagaatcgag ctgtggggaa tccgaatagg tttggcaaaa
     1681 accgaacttt ccttcgagca cgctctcggg cccggcaaaa cctttgctcg ccagcagggc
     1741 ggcagttacg ccgccctcgg cggctttgcc gagatgcagt cgcttgacca tagcgccctc
     1801 gcgacagcgg gaaaattcga tcagtccgcc ggagtaggaa gcagcgattc ccagcgcgtt
     1861 ggccatcttt ttctcgtcca gacgcagcag ccacccggcg gcaacggccg cgccgaaggt
     1921 tccggtcagc cctggtgcgt gaaagcctct tctctcgagg gagttgcctg cggcgatgcc
     1981 gattcgcgac atgacctcgc atccggcaac aaacgcgctg agcaatgatt tgccatcggc
     2041 tctcttattt tccgccatcg ccagggccgg aacaaaggct gtagcgccgg gatgaacacc
     2101 ggcgccgggc tggcgcacgt tgtcgagttc gaaagcatgt gccatcacgc cattggccaa
     2161 cgtcgcctga gctggctgcc gggaaaactt cgtaccgagg acagtcgacc gtccgcgcaa
     2221 accggaatca tgaacgaatc cgataaccgt gtggctccac ggcttcgtcg aaccatacaa
     2281 acagacggcg agagtatcta gaacacatgc cttggctcga tcgagcactt cggccggaat
     2341 atcttggtac tttagctcga gcgcaaagcg cgccaagcgt cgggttggat tcatcaacac
     2401 gcccctttgc ggcctccggt ttcagcaact cagctattga tccggcgaac agcgtcactc
     2461 tggatcactc acgcgagctt tgccggcaat tccgctcaat gaatttgttt gccgaaatac
     2521 gacgagaaaa cagttgtaag tgaagtgatg gagcacgtcg gaatcattag ccagtagtcc
     2581 gtcaacgttt gtatctgcta ggctttggcc tcgggctttt gtatcgcgcc gtcggaacga
     2641 tctctgccac ggtaccgccg ggcgcgcgaa acttgatcgg aaccactccc ggatcactga
     2701 tgatttcgca gccgaatttt tccaaatcag cggcggattt gaccatatcg tccacttcaa
     2761 tggcgaagtg atggatgcac ggtcccaacc cggacgcgcg ggcttccgcc gaatcctcgc
     2821 tgtcgtactt gatcaaagca agatcaatag tgccgtcagt gagatggcgc gaggtatgat
     2881 ctctgacctg acccgtttcc atttccacaa agccgaagcc tttttcgtaa aattccgtcg
     2941 ccttctccag gtcatcgact ttgagcgcaa tatgaacgat gcgaggcata agccctcctt
     3001 tatacggctg gcgccgtgta acacagtgac gaagagcaaa tcaagcggcg ccggcgattt
     3061 ccaccggcag cgcaaaatgg atattctcct cgatgaaacc gttctgagtc acctgcgcgc
     3121 cctgctggcg aaagtactca gcggcttgtt gcaccaaata ttccggtacc gatgcgccgg
     3181 aagtaatccc cactcgcata accccttcca gccacgcggg atcgatggcg tgatgatcgt
     3241 cgatcaggta ggcaggtttt ccttgatctc ggacgacgtt gcagagctga ttggagtttt
     3301 cactgttacg tgaaccgatg accagaacca ggtccacccg ttgggcaagc tgttttaccg
     3361 cgtcctgacg gttttgcgtg gcatagcaaa tgtcctcttt ggctgcggta atcaaccggg
     3421 gaaagcgtct cctcaaaatt tcgacaattt cccgcgtatc gtcaacgctg agcgtcgtct
     3481 gggtaagaac gacgactctg tcggggtgcg cgaccctgat ggtctcggct tcggcggcgc
     3541 tacaaacgat ttggatgttt tccggagctt ctccgcgggt accgacaatc tcgtcgtgat
     3601 tttcgtgacc gacgagaata atcgaatacc ccatagcggc gaacttgcgt acctcccggt
     3661 gcactttctc caccagtgga caggtcgcat cgacaatgat cgcgagg
//
LOCUS       DASULZ010000834         3898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1416910, whole genome shotgun sequence.
ACCESSION   DASULZ010000834 DASULZ010000000
VERSION     DASULZ010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416910"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VFY96_17135"
     CDS             <1..823
                     /locus_tag="VFY96_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608254.1"
                     /translation="GRNAPIAFLVREDLDAFLEPAAERLEAIPTLSAMALEVAHYLEK
                     HGASFLDDIARGTGLLKVKVEEGLWQLVAHGLATGDGIAGLRVLLTPEHKRLDRRRSL
                     RIISGGRSSERAMPVGRWSLWRNRSSIGALSGEAVLEQRALQLLRRYGIVFRDLLARE
                     TNLASWRALLGIYRRLEARGEIRGGRFVEGFVGEQFALPEAIERLRTIRRDSSHQQAV
                     IVSAADPLNLIGVITPGARLSSYSNQFVAYEDGVAVGVGLLGELLSQLQHAATRS"
     gene            complement(925..1212)
                     /locus_tag="VFY96_17140"
     CDS             complement(925..1212)
                     /locus_tag="VFY96_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608255.1"
                     /translation="MDDVKSQMEGVTRVYNELDNKISDKGSVQNLFGMNRKIRQALES
                     ISIGELDSMLAEIPRAKEGLTRLQEDVVEIRVLKEVLSSPASKQVNGQAQL"
     gene            1392..3614
                     /locus_tag="VFY96_17145"
     CDS             1392..3614
                     /locus_tag="VFY96_17145"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5608256.1"
                     /translation="MNEIRTIGIASPRVEGEEKVSGSAIYAEDIALPEMLWAKVLRSP
                     VAHARIKKIDVTEAAKLPGVKAVITGADLAGARIGKKIVDMPIVAETVVRYIGEKVAA
                     LAAETEAIAEAALDLIDVEYEDLPVLTDPLRAAQPDAPLLHPEVAQYKGLLHKIDVPS
                     NVFVHLKWKKGEVEEGFRQSDEIIENIFTVPAVHQAYIEPHASLVRLRSDGGADIWSS
                     NKSPFALREQLGNALQIPAASLIIHPCYVGGDFGGKGDGNDVAVCYALAKASGRPVKL
                     VIDYTEELTAGNPRHGAVIKVKTGVKKNGILIAQHVEFIFDSGAYGAYRPQGFLVGAH
                     DAPGPYRVPNCLVEEKYVYTNKMPCGYMRAPGHLQAMFACESQADLVARHLRMDPVQF
                     RRMNFMHDGDTAPLGEVIAHVKATETLMKAVDASGYARPKQKNRGRGCAIADWVSKGG
                     ESYAFIKIDSGGVVTLSSAVTDTGPGVFTMMRQIIAEELKVSLDSIRVQMLDSCEVVK
                     DTGVRGSSSTRVHGSSALDAARRARAEILRIAGQVMNAGLDELILYDGGVTHGRAERR
                     MSYAEIVRANGGPLVAEGHYVNMADGPEASLVAQVAEVEVDSETGEVRVRELTTAHNT
                     GRILNPLTHQGQIDGAVVMGLGYGVIENLAYDESGKVLAANLGEYKIPNIKDIPPLRT
                     TILESDFGSGPYNSMSIGETALIPTAAAIANAVEDAVGVRVKSLPITAEKILSALKAR
                     "
BASE COUNT          849 a         1032 c         1182 g          835 t
ORIGIN      
        1 ggggcgcaat gcgccgattg cttttctcgt gcgcgaggat cttgatgcgt ttctcgagcc
       61 cgcggcagaa agacttgaag cgattccgac gctctcggcc atggcgttgg aggtggcgca
      121 ttatcttgag aagcacggcg cctcttttct cgatgatatt gcgcgtggca cgggcttatt
      181 gaaagtgaaa gttgaagaag gcctgtggca actggtagct catggtctgg caacgggcga
      241 tggaattgca ggactgaggg tgctcctaac accggagcac aaaaggctcg atcggcggcg
      301 cagcctgagg attatctcgg gtggcagaag ctccgagcgc gcgatgccgg tgggtcgctg
      361 gtcgctctgg cgaaatcggt cgtcaatcgg agcgttgagc ggcgaggcgg tgctggaaca
      421 gcgggcgctg cagctcctgc gccgttatgg catcgtattt cgcgatcttc tcgcgcgcga
      481 aaccaatctg gcgtcgtggc gcgctctgct cgggatctac cgcaggttag aggcgcgcgg
      541 cgaaatccgc ggcgggcgct ttgtcgaggg ttttgtcggc gagcagtttg ccttgcccga
      601 agcgatcgaa aggctgcgca cgatcagacg cgactcctcc caccagcaag cggtgattgt
      661 ttcggcggca gatccgctga atctgatagg agtgataact cccggtgccc ggctatcatc
      721 atactcgaat cagttcgttg cctacgaaga cggcgttgcc gtcggagtgg gtttgttggg
      781 agaactgctc agccaactgc agcacgcagc gacgcggagc taaccccctt ccgattctaa
      841 gtagacccac ccttgccgat cgcgaagatc gtctgatgca aactgctgcg caatcgttat
      901 gcgcgaacgg gcgtagctag cgggttaaag ttgtgcttga ccgttgacct gcttcgatgc
      961 cggggagctc aggacctctt tcagaactcg aatctcaacg acgtcttcct gcaggcgagt
     1021 cagcccttcc ttggcgcgag gtatttccgc aagcatcgaa tcaagctcgc cgatgctgat
     1081 cgattccaat gcctggcgaa ttttgcggtt catgccaaat agattctgca cgctgccttt
     1141 atctgagatc ttgttgtcca gctcgttgta tacgcgagtc acaccttcca tttggctctt
     1201 gacatcgtcc acgtgattcc tccttaagtt gcttgcgact aacaacttgt tgttgtaact
     1261 accacaaatc atagatgggg gttggaacac aagcaaaagt gcatttgcaa tatggaagaa
     1321 gaaatgaaag ttggcgaaaa atcccagcag tttttttgat tgtccggcaa tcaaccatga
     1381 ggtgaactaa cgtgaacgag attcggacca tcggtattgc cagcccacgc gtggagggcg
     1441 aagaaaaagt tagcggctca gcgatctatg ccgaggacat cgcgctgccg gagatgcttt
     1501 gggcgaaagt tctgcgcagt cccgtggcac atgcgcgcat caaaaaaatc gatgtcactg
     1561 aagccgcgaa attgcccggt gtcaaagcag tcatcactgg cgccgatctc gccggcgcca
     1621 gaatcggcaa aaagatcgtc gatatgccga tcgttgccga gacggtggtt cgctacatcg
     1681 gcgaaaaagt agctgccctg gctgccgaga ccgaagcgat agccgaagcg gcattggatc
     1741 tgatcgatgt cgagtacgaa gatttaccgg tgttgacgga tccgttgcgg gccgctcagc
     1801 cggacgcgcc gctgctccat cccgaggtcg ctcaatacaa gggcttactc cacaagatcg
     1861 acgtcccgag taatgtcttc gttcatctga aatggaaaaa aggggaagtc gaggaaggtt
     1921 tccggcaatc cgacgagatc atcgagaaca tttttacggt cccagccgtg catcaggcgt
     1981 acatcgaacc gcatgcgtcg ctggtacgat tgagatctga cggtggcgcg gacatttggt
     2041 cttccaacaa aagcccattt gccctgcgcg agcagctcgg gaacgcgctg caaatcccag
     2101 cggccagtct gatcattcat ccgtgttatg tcggtggaga tttcggtggc aaaggcgacg
     2161 gcaacgatgt cgctgtctgc tatgcgctcg caaaggcgag cggccgaccg gtcaagctgg
     2221 tgatcgatta tacggaagag ttgaccgcgg gaaatccgcg tcacggcgcc gtgatcaaag
     2281 taaaaaccgg cgtgaagaaa aacggtatcc taatcgcgca gcacgtcgaa ttcatcttcg
     2341 acagcggcgc ttacggcgct taccggccgc agggattttt agtcggcgcg cacgacgcgc
     2401 ccggtcccta ccgggttccc aactgcctgg tggaagaaaa gtacgtttat acgaacaaaa
     2461 tgccgtgcgg ctatatgcgg gcgccgggcc acctgcaggc gatgtttgcc tgcgaaagcc
     2521 aagccgatct cgtcgcgcgt catttgcgca tggatcccgt gcagtttcgc cgcatgaatt
     2581 ttatgcacga cggcgatacg gccccgctcg gcgaagtgat cgctcatgtc aaagcaactg
     2641 aaacattgat gaaggcggtg gatgcttcgg gctacgcccg gcccaaacaa aagaatcgcg
     2701 gccgcggctg cgcgatcgcg gattgggttt ccaaaggcgg tgagtcctat gcatttatca
     2761 aaattgacag cggtggcgtg gtgacgctat cgtccgcggt gaccgacaca ggccccggtg
     2821 tcttcacgat gatgcggcag atcattgccg aagaactcaa agtttcgctc gactcgatcc
     2881 gagtccagat gctcgattca tgcgaagtcg tcaaagatac cggggtgcgc ggcagcagca
     2941 gcacgcgggt gcacggcagc tctgctctcg atgccgctcg aagggcgcgg gcagaaattt
     3001 tgcgcatcgc gggccaagtc atgaacgccg gcctcgacga actgatcctg tatgacggtg
     3061 gtgtaactca cggacgagcc gaacggcgca tgagttatgc cgagatcgtc cgcgccaatg
     3121 gcggcccgct ggttgccgaa gggcactacg tcaatatggc ggacggtccg gaggcttcgc
     3181 tcgtggctca ggttgccgag gtggaagtcg acagcgagac cggcgaagtg cgcgtcaggg
     3241 agctcaccac tgcgcacaac acgggcagaa tcttgaatcc gttgacccat cagggacaga
     3301 tcgacggcgc agtggtgatg ggattgggtt acggggtgat cgaaaacctc gcttacgacg
     3361 aaagcggtaa ggttctggcg gcgaatttgg gcgaatataa gattccgaac atcaaggata
     3421 ttccgccgct cagaacgacg atcttagaat cggatttcgg tagcggtccg tataacagca
     3481 tgagcatcgg cgaaacggca ttgatcccaa cggcggcggc catcgccaat gcggttgagg
     3541 acgcggtggg agtgcgggta aaatcgctac cgatcacggc ggaaaaaatc ttgagcgcgc
     3601 tcaaagcacg gtagcgaaca tcatattttt ccgcccctca gagcgggcac ctggagtctt
     3661 tttatcttca tccatgcgca gcggcattcc cggaccgatt acgcgagtga tcaatgcgac
     3721 gcggtattca ttaaatggcc tccgagacgc gtggcacagg caagcgcctt ttcgctatgc
     3781 atgttatgta ttggtcttgg tgatcccgct agggtggcgc tgcggagaaa ccggatttgg
     3841 gctccactgc cgttttctgt tccattgttt tgtttgcgat ggtttgggtg atcatttg
//
LOCUS       DASULZ010000835         3246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1517193, whole genome shotgun sequence.
ACCESSION   DASULZ010000835 DASULZ010000000
VERSION     DASULZ010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517193"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VFY96_17150"
     CDS             <1..813
                     /locus_tag="VFY96_17150"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5608257.1"
                     /translation="REYRSSYRAELSSTERIIAGVWHGKVAADTEPVPVSVEKGIAEN
                     LKVGLGDALEFEIQGVPIKTRVASIREVDWQRVQPNFFVVFPEGPLENAPQFYAVVAR
                     AESAEAAARLQRAVVERFGNVSMIDLSLILNTLNAILGKVSAAVRFVALFTIVTGFAV
                     LASAVIGSRAERVRESILLRTLGAPRAQILGVIAAEYLFLGGIAGAAGTLLGVTATWG
                     LSLYFFAVPALIAPIPMVVIFLIVTAATVITGALGSRGIFRSSPLEALRAEA"
     gene            869..3246
                     /locus_tag="VFY96_17155"
                     /pseudo
     CDS             869..3246
                     /locus_tag="VFY96_17155"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
BASE COUNT          692 a          852 c          978 g          724 t
ORIGIN      
        1 cgagagtatc gctcgagtta tcgcgccgag ttgagcagca cggaaagaat tattgcgggc
       61 gtgtggcacg gcaaagtcgc ggccgatacg gagccggtgc cggtttccgt cgagaagggc
      121 attgcggaaa atctcaaggt cggattaggg gatgcgcttg agttcgaaat ccagggagtg
      181 ccgatcaaga cgagagtggc gagcattcga gaagttgatt ggcagcgcgt gcaacctaat
      241 ttttttgtgg tttttcccga agggccgctc gagaatgcgc cgcaattcta tgctgtcgtc
      301 gcgcgtgccg agtccgctga agcggcggca cgactgcaac gagcggtggt cgagcggttt
      361 ggcaatgttt cgatgatcga tctcagtctg atcctgaaca cgttaaacgc cattctcggc
      421 aaagtctcgg ccgccgtgcg atttgtcgct ctgttcacga tcgtaaccgg ctttgccgtc
      481 ctggcgagcg cggtgattgg cagccgagcg gagcgggtcc gagaaagtat tctgctgaga
      541 actttgggcg cgccgcgggc gcaaattctc ggtgtgatcg cggcggaata cctgttcctc
      601 ggcggcatag ccggcgcagc cggcacacta ctcggcgtca ccgcaacctg gggcttgagc
      661 ctttattttt ttgctgtgcc ggctttgatc gcaccgatcc cgatggtcgt catttttctc
      721 atcgtcaccg ccgcaactgt tatcaccggc gcgctcggca gccgggggat ttttcgaagt
      781 tcgccgctcg aggcgctgcg cgcagaagct tgattcgatc tgcatagccg cacaaacgca
      841 cttagaagat cattcataag gagtgagcat gcctgaagct aaatcgtatc gcttgcccac
      901 atcggtaatc ccggaacgct atgagctgaa attgactccg gacctgaccc gttggacgtt
      961 cacaggtcag gagaaaatct cggtgcaaat gaccgaggcg gtgcgcgata ttgtcttgaa
     1021 cgccgcagaa ttggagctcc atcgagtgtc gctgaagcgt gctgatggga gagtggtcga
     1081 aggccgggcc tccttggatc ccgagaatga acgagcaaca ttgagttttc ctgagattct
     1141 gccggccggc agcgctgaat tgctgatcga gttttccggg atcctgaatg acaaactgca
     1201 cggtttttac cgcagcacct ataagggagc ggatggccaa gacaagccgc tggcatcaac
     1261 gcagttcgaa tccaccgacg cgcgccgcgc atttccttgt tgggacgagc cggcgctcaa
     1321 agctgtgttt caggtgacac tcgtggtcga tgaaaagctc acagcgatct ccaatgcccg
     1381 ggtgatgcgc gaaaaacgtc tcgagagcac gggaaggaaa gaggtggtct ttgccgattc
     1441 gataaaaatg tccacttacc tggtggcatt tgtcgtcggt gaattcgaag cgactgaggc
     1501 cgtcacggca gccggagctc cgctccgagt atgtgcggtt ccggggaaaa aattgttgac
     1561 tcgtttcgcc ctcgatatcg ggaaagcgtc gctggattat ttcagccgtt attacgatat
     1621 tccctaccca ggagacaaac tcgatctgat tgcaattccg gattttgctt ccggcgcgat
     1681 ggagaatctc ggcgcgatta cctttcgcga aactgcattg ctcgtcgatg cggaaaaggc
     1741 aacgcgcgcg gagcttgaac gggttgctga cgtggtatcg catgaaaatg cccatatgtg
     1801 gtttggcgat ctagtgacga tgaaatggtg gaacggactt tggcttaatg aagccttcgc
     1861 cactttcatg gaaatgctcg cggtggatgc ttggaagccc gagtggcgcc gctgggataa
     1921 ttttaccata tcacgtgcgg cggccatgca ggtcgacggt ctgaagagca cacggtctat
     1981 cgaatttccg gtggagcggc ccgaggaagc cgcgggaatg ttcgatgtgt tgacctatga
     2041 gaaaggcgcg tcagtgctgc gtatgctcga gcaatatctc ggcgcggagg cgtttcgcga
     2101 tgggatccgc gtctatctgc aacgtcacca gtacgccaac gcagagacga ccgatctttg
     2161 ggatgcgctc gagaagtcaa ccgagcagcc ggtgcgcact cttatggaca gctggatttt
     2221 tcagcccggc tacccgctca tcactgtgga aaagcacggc cgtggtctac gtttgactca
     2281 acagattttt cgctatcttc aggacggctc gacctccggt caacgctggt atgtgccgat
     2341 ttttcttcgc gccggcagta aatcgggcat ttttcggcag acgcttctct tgactgaaaa
     2401 cgagcagacg ctggagttcg agaactcgcc cgactgggtc gtcgtgaacg ccggcggtca
     2461 tgggttctac cgcgtaggtt acagccagga gctgctcgat gcgctgaagg gttcacttaa
     2521 tgaccagctt tcttcggtcg agaagttcgg cttagtcaac gatagttggg cggcaaccct
     2581 cgctggccta gttgcgcctt ctgactacct ggcgcttatc gagttaatgc gccacgagag
     2641 ggacctcaat atctgggcga cgatcatcgg ctcgtgccat aatctggagc gtatcctcga
     2701 cgaggatcaa tgcgacaaac tggcggaacg ggtccgaacg ctggtcgggc cggcggtgga
     2761 acggttcggc tggtcgacac gagaagggga aaacgagctc gagagccagc tgcgcggcga
     2821 tttgattgcc gcccttggca ccctcggcca agacaaaacg tgacaacaac gtgcgcgcga
     2881 gatcttcgcc ggctacgaga aggcgccgga tgcggtcgat cgcaatatta taccagcgct
     2941 ggtcgcgatt gtggcgcata ccggcacgat ttccgattac gagaatttct accgaagatt
     3001 caaggacgcc gcgacgccac aagaagaaac ccgcttcctg tttgctctgg caaattttcg
     3061 tgcgccggag ttgatcgatc gcactcttga tctcactatt agcggcgaag tgcgtacgca
     3121 aaacgcgcct tatttattac gaggcattct tctcaaccgt aaagcgcgcg ccaaagcctg
     3181 gtcattcatg aaggcacact gggaggaaat gctgcgccag tacccggaca attcgattcc
     3241 gcggat
//
LOCUS       DASULZ010000836         3201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_197691, whole genome shotgun sequence.
ACCESSION   DASULZ010000836 DASULZ010000000
VERSION     DASULZ010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197691"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="VFY96_17160"
     CDS             complement(<1..1259)
                     /locus_tag="VFY96_17160"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5608258.1"
                     /translation="MSYQVIGQPVARIDNAGKVNGEARYTADVLLPGTLWAKTLRSPY
                     AHARIVRIDTSRAEKAEGVRAVLTGNDVRGILYGRRYRDISILAQERARFMGERVAAI
                     AADTLEQAEQALDLIEVHYEELPAVFDPEAALQEGAPIIHPDVATYKGLPKALEAPSN
                     RFAYDVFNRGDVDTGFARAEVIVENAFSVARVHQAFLEPHCCLVWIDEQERVQMWSPN
                     KAPQGLKESMCNAFKIAPEKVCIHPVAIGGDFGGKGAPIDEPICYLLALRTGRPVKMI
                     MEYREEFIAGAPRHAAVMKLKTGVKRDGTIVAHQMDAFLDAGAYGGFRPGAVVGGIAH
                     AGGCYRAEHSRISVSRIYTNNLPGGQMRAPGEPQGFFAAESHMDCVARKLGMDPVEFR
                     LKNLIEEGDETLTGGHYRGIKAKETLRA"
     gene            complement(1367..2347)
                     /locus_tag="VFY96_17165"
     CDS             complement(1367..2347)
                     /locus_tag="VFY96_17165"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX5608259.1"
                     /translation="MMTDLAPPQIRAITGLELAAGVFNKCSERLATQLSISLTSGRRD
                     QGPTEFRIGINGDIYGGMHAPIEVGTRRVDIAYVNPAALVTMAYRGKGYYKRKMPLRV
                     LGCFPSWDRIALVVSKDLGVKSLYDVAERKLPLHVSTRMAGVNNGTYYTISTILSFYG
                     LSFDKIKRWGGKVQECGRPFAPERLKAIAKRSINAVFDEGVSTPGGWLDQALDNGYEV
                     IPLEPAIVKKLEQIGYTRTALPKSRYPQLNDDALTIDYSGWALVTHRWLPDDIAYATI
                     QTIDEKQKVIPIDDDRPLEMRNLCRGTEKCPLQIPLHPGALKYYREKGYL"
     gene            2551..>3201
                     /locus_tag="VFY96_17170"
     CDS             2551..>3201
                     /locus_tag="VFY96_17170"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5608260.1"
                     /translation="MKTESMFKVRKEDLHNFTREENEALTRVGPATLMGNLMRQYWIP
                     IVPTAHLKEPGGKPLRVRLLGEDLVLFRTLASQVGLIGAFCPHRLAPLYFGRVEQDGL
                     RCAYHAWKYAPNGKCIEMPNIPMDQQFKEDVQHPGYPCIERGGIIWTYMGLAKDLPEL
                     PDFEFLRVPDEHRIYRLFYQEGNYLQVLEGGIDPTHVMWLHSPYDLSDEEMNAQQPA"
BASE COUNT          687 a          962 c          868 g          684 t
ORIGIN      
        1 gctcgaagcg tttctttcgc tttgatgccg cggtaatgac caccggtcag cgtctcatcg
       61 ccttcttcga tgagattctt cagacgaaac tcgacaggat ccatgccgag ctttcgtgcc
      121 acgcagtcca tgtgcgactc agcggcaaaa aatccttgcg gctcgccggg cgcacgcatc
      181 tggccaccgg gcaaattgtt ggtgtaaata cgcgacaccg agatgcgcga gtgctccgcg
      241 cggtagcagc cgcccgcgtg cgcaatgccg ccgaccacgg ctccgggccg gaatccgccg
      301 taggccccgg cgtcgagaaa cgcgtccatc tgatgcgcga caattgtgcc gtcgcgtttg
      361 acgcccgttt tcaacttcat caccgctgca tgccgcggcg cgccagcgat aaactcttcg
      421 cggtattcca tgatcatctt gaccggccgg ccggtacgaa gcgcaagcaa gtagcaaatc
      481 ggctcatcga tgggcgcgcc cttgccgcca aaatcgccgc cgatcgcgac aggatgaatg
      541 cagactttct ccggggcgat tttaaacgca ttgcacatgc tctctttcaa tccttgcggc
      601 gctttgtttg gcgaccacat ttgcacgcgc tcctgctcgt cgatccaaac caggcagcaa
      661 tgcggctcca gaaatgcctg gtggacgcgc gccacactga aagcattctc gacaatcact
      721 tccgctcggg cgaaaccggt atcgacatcg ccgcggttaa atacatcgta ggcaaaacgg
      781 ttgctcggag cttcgagcgc cttcggaagt cctttataag tggcgacatc tggatgaatg
      841 atcggcgcgc cttcctgtag cgccgcctcc ggatcgaata ccgccggaag ctcttcgtaa
      901 tgcacctcga tcagatcaag agcctgctca gcctgttcga gcgtgtccgc cgcgatcgcg
      961 gcgacgcgct cgcccatgaa gcgcgcgcgc tcctgggcca aaatggaaat gtcccgatag
     1021 cgccggccgt aaagaatgcc gcgaacgtca ttgccggtca ggacggcgcg tacaccctca
     1081 gctttttcgg ctcgcgatgt atcaatgcgc acgattcgcg cgtgcgcgta cgggctgcgc
     1141 aaggtcttcg cccatagcgt tcccggcaac agcacatcgg cggtgtagcg cgcctcgcca
     1201 ttgactttgc ccgcgttgtc aatccgcgct accggttgtc cgatcacttg ataactcaca
     1261 aaatcctcct accgaacaat aatggagtgc tggagtactg gagtactgga gtattgcgcg
     1321 tccgaaaacc catcactcca ttactccagc acggcatcag cccaatctaa aggtatccct
     1381 tctcccggta gtacttcagg gcaccgggat gaagcggaat ctgcagcgga cacttttcgg
     1441 tgccgcggca aagatttctc atttccaacg gccgatcatc gtcgatcggg atgactttct
     1501 gtttctcgtc gatcgtctga atcgtcgcat acgcaatatc gtccgggagc cagcgatgcg
     1561 ttaccagcgc ccagccgctg taatcgattg tcagcgcgtc gtcattgagt tgcggatagc
     1621 ggcttttcgg caatgcggtt ctcgtataac cgatttgctc gagctttttg acgattgccg
     1681 gttcgagggg aatgacttcg tagccgttgt cgagcgcctg atccaaccag ccgcccggag
     1741 tgctcactcc ttcatcgaat accgcgttga tcgagcgctt ggcaatcgcc ttcaatcgct
     1801 ccggcgcaaa cggtctgccg cactcttgaa cttttccacc ccagcgtttg atcttgtcga
     1861 acgataatcc gtaaaaggaa agtattgtcg agatcgtgta gtaggttccg ttgttgacgc
     1921 cggccatgcg cgtcgatacg tgcagcggaa gctttcgttc ggcaacgtcg tagagcgact
     1981 tcacgccgag atccttcgag accacgagtg cgatgcggtc ccaagaaggg aaacagccga
     2041 gcacgcgcaa tggcattttc cgtttgtaat aacctttgcc ccggtaagcc atagtcacca
     2101 gcgccgccgg attgacatac gcgatatcga cccgccgcgt gccgacctcg atcggcgcat
     2161 gcatgccgcc gtaaatgtcg ccgttgatac cgatacgaaa ctccgtcggc ccctgatcgc
     2221 gccggccgga ggtcaatgaa atcgagagct gggtagccaa tcgctccgag cacttgttga
     2281 acacgcccgc cgccaattcc aatccggtga tcgcgcgaat ttgcggcggc gcaagatcag
     2341 tcatcatagt cgttggtatc tcccgaataa ttgatcgctt gctgcgcaat atcaaaaacc
     2401 gggttgttat ggtagaagca aaaccgcgca aactggcgca caccgtctag ccaaacggga
     2461 gatgtaacgg gcgaccggcg atcgcccgtt ggatgatcac ccaacaatct gcgacgtaaa
     2521 accctcatga cagtcgcata ggaaacaggc atgaaaaccg aaagcatgtt caaagttcgc
     2581 aaagaagatc tccacaactt tacccgcgag gagaacgaag cactcactcg cgttggcccg
     2641 gcgactctga tgggaaatct gatgcgtcag tactggatcc caatagttcc cacggcgcat
     2701 ttgaaagaac cgggcggaaa accgctgcgc gttcggcttc tcggcgaaga tctagtgctc
     2761 tttcgcacgt tggccagcca agtcgggctc atcggcgcct tttgtccgca tcgacttgcg
     2821 ccactctatt tcggccgcgt cgaacaagac ggcctccgct gcgcttatca tgcgtggaaa
     2881 tacgcgccga acggtaaatg catcgagatg ccgaatattc ccatggatca acagttcaag
     2941 gaggatgtgc agcacccggg ctacccgtgc atcgagcgcg gcggcatcat ttggacttac
     3001 atgggtcttg cgaaggattt gccagagctg ccggatttcg agtttctccg cgtaccggac
     3061 gagcaccgaa tttatcggct gttctaccag gagggaaatt atctccaggt gctcgaaggc
     3121 ggtatcgatc cgacccatgt gatgtggctg cactcgccct atgatttatc cgacgaagag
     3181 atgaatgctc aacagcccgc g
//
LOCUS       DASULZ010000837         3467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_706179, whole genome shotgun sequence.
ACCESSION   DASULZ010000837 DASULZ010000000
VERSION     DASULZ010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706179"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="VFY96_17175"
     CDS             complement(<1..1117)
                     /locus_tag="VFY96_17175"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX5608261.1"
                     /translation="MKIAEIEIFELQNIPITPPLFKAPVRAAVRVLEIKTDNGITGIS
                     QIGGFMHSASIAFIRNELAPFLNGKDPLETERLMHQMLWKFNTRAHGGVWNFAVSAID
                     VALWDIKGKFYNVPVWRLLGAAQQSVPAYITFGLRAYSKDALAEAARYWVAKGQTRLK
                     IQVGRLNARGEMDPSGAGAEHREDNPAEDEGRVRAVRDAVGDTVELMVDANCLMKYDA
                     ALRWCKRFEPYNLIWFEEPIVHNDRKLLAELRKQTSIPIAAGQWDSFFALSDLVKERA
                     VDFLNIHVLSVGGFTMGMKAAGVAHAFNLPLGNGDHFDLHLQAAVPNGWRAEFHVNNW
                     LTWNVLYKEVPEPVDGWVTLSERPGLGLELNADAVKEY"
     gene            1196..1510
                     /locus_tag="VFY96_17180"
     CDS             1196..1510
                     /locus_tag="VFY96_17180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608262.1"
                     /translation="MGKPLNTDPWFIWVPALSGNDITQIRLPDRVVLADLKNERARVV
                     ILVCRVLRLGIVDNELISGRTLKVMQRKGPYELALSGLNTIEITTITARPNMEVSITL
                     LA"
     gene            1720..2124
                     /locus_tag="VFY96_17185"
     CDS             1720..2124
                     /locus_tag="VFY96_17185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608263.1"
                     /translation="MLEKIKRSWQSFKADPPGKRFQQQFKRRHEVARTPLQKALVVGG
                     GVLLTGVGILLFFLPGPGVLFVLLGAVLIAQQSLTVARALDWTEIRLRRLLALSFGAW
                     RHFSAALKIFLVGLAVGAAAAVGFGVFKFLTA"
     gene            complement(2150..2326)
                     /locus_tag="VFY96_17190"
     CDS             complement(2150..2326)
                     /locus_tag="VFY96_17190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608264.1"
                     /translation="MEKVGGRRALLVISPSVAKMFLLERIKSGAKCVGVFDQVNIRLM
                     ASRVLYFVIFDPNT"
     gene            complement(2378..>3467)
                     /locus_tag="VFY96_17195"
     CDS             complement(2378..>3467)
                     /locus_tag="VFY96_17195"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5608265.1"
                     /translation="SDRVARDLPMTSRWSAAFTILLIALGASAQLGHSAAPFYEGKTI
                     RIIVGTSPGGGYDTYTRLIARHFGKYIPGKPTIIVDNMPGAGGLVSANHLFKVAKPDG
                     LTVGHFVGGQFLQQLLGKPGIEFDALKFEYVGVPAQDDFVFRVAKVTGITDIEQWLAS
                     KSEVKFGAIAAGDGTYDTPKVAAVALGLPIQVVSGYKGTAPIRLAFDSGEVGGLSNSW
                     QSFRATWRKELDTGDLRIVLQLSAKPHPDLMKVPLAANFAKTDEAQKLIQAVAQAHGA
                     AVRPYVLPPGTPKDRVETLRRSFMAAMKDPELLNEAAKANLEMNPGSGEDLERNVKEL
                     LRLDPALVNRLKEILKERIDGSNLVSNP"
BASE COUNT          723 a          991 c          929 g          824 t
ORIGIN      
        1 ggtactcttt caccgcatcg gcattgagct ccaaccctag ccctggccgt tcactcaatg
       61 ttacccatcc gtcgaccggc tcagggactt ctttgtagag tacgttccat gtcagccaat
      121 tgttgacatg aaattcggcg cgccacccgt tgggaacagc ggcctggaga tgaagatcga
      181 aatggtcgcc gttgccgagg ggaagattga aagcgtgggc cactccagcg gctttcattc
      241 ccatggtgaa gccaccgact gaaagaacgt gaatgtttaa aaaatcgact gccctttctt
      301 tgaccaggtc ggagagcgca aaaaaactgt cccactgtcc tgctgcgatc ggaatgctcg
      361 tctgtttgcg caactccgcc agcaacttgc gatcgttgtg gacaatgggc tcttcaaacc
      421 agattaaatt gtacggctcg aaccgtttgc accagcgcag cgccgcgtcg tatttcatca
      481 gacagttggc atcgaccatc agctcgacgg tgtcgccgac cgcgtcccgg actgctctca
      541 cccggccttc atcctccgcg ggattatcct cgcgatgttc cgcacccgct cccgagggat
      601 ccatctcacc gcgagcgttc aatcggccaa cttgaatttt taaccgcgtt tgacctttgg
      661 ccacccaata cctggccgct tccgcaagcg catcctttga gtaggctctg agtccgaacg
      721 taatgtaggc tggaaccgat tgttgcgccg cgccgaggag ccgccagacc ggcacgttat
      781 aaaatttgcc tttgatgtcc cagagcgcga catcgatcgc gctcaccgca aagttccaga
      841 ctccgccgtg cgctcgagtg ttgaacttcc acagcatctg gtgcatcaac cgctcggtct
      901 ccagagggtc ctttccgttc aaaaatggcg cgagctcgtt gcggatgaaa gcgatgctcg
      961 cggaatgcat gaacccgccg atttgactga ttccagtgat gccgttgtcg gtctttatct
     1021 ccagcacgcg tacggccgcg cgcaccggcg ctttgaatag cggcggcgtg atcgggatat
     1081 tttgcaattc aaagatctcg atttctgcaa ttttcattga cggccccctt tccgcacttg
     1141 tttcttacgg caaaccgagc gcgagcgtca atcgaaacaa cggaaagtga acccgatggg
     1201 gaagccgctc aataccgatc cgtggttcat ttgggttccg gcgttgtccg ggaacgacat
     1261 aactcagatt cgcctgccgg atcgtgttgt ccttgccgat ctaaagaacg aacgcgctcg
     1321 agtcgtgatc ttggtgtgcc gggttctgag gcttggcatc gtcgacaacg agctcataag
     1381 tggccgtact ctgaaagtta tgcaacgtaa aggaccatac gaactcgcat tgagtggcct
     1441 caacacaatt gagatcacaa cgatcactgc ccgccccaat atggaagtca gcatcactct
     1501 tctcgcataa acttgttgtt tttctcgctc ggcgatttaa atagctgccg tatcagcacc
     1561 gcaacattac actcgatttc tcgtctgcgt ctcggcgccg cggatctttg ggtgctcacg
     1621 tctatagcca tagttttttc tatgctagct tttatgggat gaatgaagat tgtcttgccc
     1681 gacccggaac gtgagacgga agtgaagcgg tgaccgccga tgctcgaaaa gatcaaacgt
     1741 agctggcaaa gttttaaagc ggaccctcct gggaaacgtt ttcagcagca gttcaagagg
     1801 cgtcatgagg tcgcacgcac tcctctgcaa aaggcgctcg ttgtcggcgg tggcgttttg
     1861 ctcaccggag ttggaatcct tttgtttttt cttcccggac ctggcgtgct ttttgtgctg
     1921 ttaggggctg tactgatcgc gcagcaatcc ttaaccgtcg ctcgtgcact ggactggacc
     1981 gaaattcgcc tgcgaaggct gctggctctg agcttcggcg cgtggcggca cttctctgcg
     2041 gcgctgaaaa ttttcctggt cggtttggct gtcggcgccg cggcagccgt cgggtttggc
     2101 gtgtttaagt ttttgactgc ttgaggcggt tgaagcggaa tgaccgctgc taggtgttcg
     2161 ggtcaaaaat cacgaaatac aaaactcttg acgccatgag tcgaatgttg acctgatcga
     2221 aaacacccac gcacttcgct ccggatttga tccgctccag caaaaacatt ttggccaccg
     2281 acggcgagat caccagcaat gcgcgtcttc cgccaacctt ctccatttct ttgggtaatt
     2341 tctcgatcga cgccagccgg aaaacgatcc gctcgactca cgggtttgaa acaagatttg
     2401 agccgtctat ccgctctttc aaaatttctt tgagtcgatt aaccagagcg ggatcgaggc
     2461 gcagaagctc tttgacgttg cgctcgaggt cttcgcccga tcctggattc atttccagat
     2521 tggctttggc ggcctcgttg agcagttccg gatctttcat cgccgccata aaagaacggc
     2581 gcagagtctc gacccgatcc ttcggcgtgc cgggcggcag gacgtacgga cgcaccgccg
     2641 cgccgtgagc ttgcgccacg gcctggatga gtttttgcgc ctcgtctgtt ttggcgaaat
     2701 tcgcggccaa aggcaccttc atgagatccg ggtgcggctt cgcgctaagc tgaagcacaa
     2761 tcctaaggtc gccggtgtca agctcctttc tccatgtcgc gcgaaacgat tgccacgaat
     2821 tgctcaggcc gccgacctcg ccactgtcga atgccaagcg tatgggcgcc gtccctttat
     2881 accccgagac gacctggatc ggcaggccaa gcgcgaccgc cgcgaccttc ggagtgtcat
     2941 aggttccatc gccggctgcg atggcgccga acttgacctc tgatttcgat gccaaccatt
     3001 gctcgatgtc tgtgatgccc gttaccttgg ccaccctaaa gacaaaatcg tcctgggcag
     3061 gtacgccgac gtattcaaac ttgagcgcat cgaattcaat gcccggcttg cccaggagct
     3121 gttgtaggaa ttgtccgccg acgaaatggc cgacggtgag accgtcgggt ttcgctactt
     3181 tgaagagatg gtttgccgaa accaatccgc ccgcgcccgg catgttgtcc acgatgatag
     3241 taggtttccc gggaatatat ttgccgaagt ggcgggcaat caggcgagtg taggtatcgt
     3301 atccacctcc aggcgaggtt ccaacgataa tgcggattgt cttgccttca taaaaaggcg
     3361 cggcgctatg accgagctgc gcgcttgcac cgagcgcaat cagcaaaata gtgaaagcag
     3421 cgctccaacg cgacgtcata ggcaagtctc tcgcaactcg gtccgac
//
LOCUS       DASULZ010000838         4431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_97650, whole genome shotgun sequence.
ACCESSION   DASULZ010000838 DASULZ010000000
VERSION     DASULZ010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97650"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFY96_17200"
     CDS             complement(<1..227)
                     /locus_tag="VFY96_17200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX5608266.1"
                     /translation="MLLINNEVVAQILNIKGCMEALETGYRDLIDERAAYRGRYDFFV
                     PNDDPKLMYRWGTMEGASRSFETFAIRMKSD"
     gene            complement(338..778)
                     /locus_tag="VFY96_17205"
     CDS             complement(338..778)
                     /locus_tag="VFY96_17205"
                     /inference="COORDINATES: protein motif:HMM:NF018391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEX5608267.1"
                     /translation="MVIYNLLCKKKHRFEGWFPSFEDFQKQRDKKLISCPSCGTTKVE
                     KVPHACAVHLKKEEPLPAKKPRQEPPAPVSPTEFKEMLLKVHHYVKENFEDVGSRFAE
                     EARQIHKGEAEERPIHGTATIQETKELAEEGVPFLPLPKPELDS"
     gene            971..2119
                     /locus_tag="VFY96_17210"
     CDS             971..2119
                     /locus_tag="VFY96_17210"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5608268.1"
                     /translation="MTVKVVQKIAQVPRERWDALVENATPFLKWDWLDSLEQSNCVTE
                     RTGWLPHHIIVEQGATLVGACPMYLKLHSMGEFVFDYEWAEAAHHAGIQYYPKMLVGI
                     PFTPVTGHRFLTAAGEERAPLVRFMGQALAKIAADNKISSVHVNFCLAEERQALEQVG
                     FFPRAGIQFHWQNRGFNSFDDYLASFRSDRRNKVKRERREVAVRGITIRAFEGEELTL
                     QRMRTMFRLYKGHVDRLYYGRQYLTREFFDDLHRRFAPHLCLMLAEREGKIIAGTFNV
                     RDDTAMYGRYWGCFEEHPFLHFNVCYYSAIEHSIRIGLERFEAGAGGSFKQLRGLDPE
                     HTTSMHYIVDGRLRRAVERFLAQEREMIQRKREMLLDHSQLKHEPQEP"
     gene            2360..3136
                     /locus_tag="VFY96_17215"
     CDS             2360..3136
                     /locus_tag="VFY96_17215"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5608269.1"
                     /translation="MKFSWKNIAEPVLFFAVLLLLWEILVEVIKVPTFILPPPRDLWI
                     AFWKKLPILGHHSFITFIEAFGGFVLSLVLGVGFAIAVVYSRHLQNTIYPLIVILYAM
                     PKSAFAPLMVIWVGYGLFSKIAIAFLVAFFPIVVNTVVGLKEIEPELLELARINRASE
                     FDVFQKIRLPNSIPYLFAGIKVAIVLSVTGAIVAEFVAANEGLGYLVLQANYNLDTAF
                     ALVILLILAVLSLGLFWLAEVIQRKMAPWSAGVREVEGSF"
     gene            3162..4172
                     /locus_tag="VFY96_17220"
     CDS             3162..4172
                     /locus_tag="VFY96_17220"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5608270.1"
                     /translation="MSLITGRGSSIIFAITVALLLFSQAPLQAAELTPATIRLDFIIG
                     GKHAPWYVALEKGFYAKRGLNATIQSSTGSADTVRTIGSGGADFGFADIGTTIVAKSR
                     GTPVQVVGQFGYVPATILWRDEGVIKGIQDLAGKSWAISPGQAQWYLMPAYCRINKID
                     FKSIKIQETAAPIQPAALATKKADFIVMYRASNDEIAEQAATKVGVKLSRIFMKDTGL
                     DIYGTGLIVKDDDVKKRPDFVRAYVEATLEGLRYARDNQEESLKILLKHKPELEPTLT
                     SLQLKNALTEVFLPFESAQVGLGYMKPEIIEKTVKVTNEYFDVGRKVAAKEVFSNQFI
                     RR"
     gene            4198..>4431
                     /locus_tag="VFY96_17225"
     CDS             4198..>4431
                     /locus_tag="VFY96_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5608271.1"
                     /translation="MPPLIALSDVSRVFTSGGTTVTALNRVGFDVQAGNFVSIVGPSG
                     CGKSTLLKIVAGLLPSSSGTVTVHGEPVRGPLAN"
BASE COUNT          947 a         1159 c         1255 g         1070 t
ORIGIN      
        1 atgtcggatt tcatccggat cgcaaatgtt tcgaagctcc gcgatgctcc ttccattgtg
       61 ccccagcgat acatgagctt ggggtcatcg ttgggcacga agaaatcgta gcggccacga
      121 taagccgcgc gttcatcgat caaatcgcgg taacccgttt caagggcttc catgcagcct
      181 ttaatattca gaatttgcgc taccacttca ttgttgatga gcagcatgaa cactccggcg
      241 aggaagacgc atctatttcg cagtcacgga gaatttgcaa gggttaaaaa aacgccggca
      301 agtgccggcg tgacctagtc ggtgagaaat tctctgctta gctgtcgagc tcgggtttgg
      361 gcaaggggag aaagggaacg ccctcttcgg cgagctcttt ggtttcttga atcgtagcgg
      421 tcccgtgaat cgggcgttct tcggcttcgc ccttgtgaat ctggcgcgcc tcttcagcga
      481 agcgggaccc gacatcttcg aagttctctt tcacgtaatg atgcacctta aggagcatct
      541 ccttgaattc ggtgggggag accggtgcgg gcggctcttg ccggggcttt tttgccggca
      601 gcggctcttc tttctttagg tggaccgcgc aggcatgggg aactttttcg acttttgtgg
      661 tgccgcaact cggacaagaa ataagctttt tgtcccgttg cttttgaaag tcttcaaaac
      721 tcggaaacca gccttcaaag cgatgtttct ttttgcagag aagattatag atgaccataa
      781 cgactcggat agtatcaata agggtccggc gccgagttgt caacccctcg atacgcgctg
      841 cgcgctactc ggggaattgg gttctccgac ggtcctgagt aggcagcgaa tagcgctgcc
      901 gtatcgaagg atgcacaaaa gtttgatcga aaaggtgccc gtgctaagat cgctgttgat
      961 gtccgaaaca atgacagtca aggtcgtgca aaaaatcgcc caagtgccgc gcgagcgctg
     1021 ggacgcactg gtcgagaacg ccacgccctt cttaaaatgg gattggctcg atagtcttga
     1081 gcagtcgaat tgcgtgaccg agcgaaccgg ttggttgccc catcatatca ttgtcgagca
     1141 gggcgcgacg ctggtcggcg cttgcccgat gtacttgaag cttcacagta tgggcgagtt
     1201 cgtcttcgat tacgaatggg cagaagcggc acaccatgcg ggtattcagt actatcccaa
     1261 gatgctcgtg gggattccat tcactccggt gactgggcac cggtttctca cagcggccgg
     1321 tgaagagcgt gcgccgctgg tccgttttat gggtcaggcg ctggcgaaga tcgccgccga
     1381 taacaaaata tcttccgtac acgtgaattt ttgcctggcg gaggagcggc aggcgttgga
     1441 gcaagtcggg ttttttccgc gcgcgggtat tcagttccac tggcagaacc gaggctttaa
     1501 ctcgttcgac gattatctcg ccagctttcg cagtgaccgt cgcaacaaag tcaaacgcga
     1561 gcggcgcgag gtcgccgtgc gcggcatcac gattcgggct ttcgaaggcg aagaactgac
     1621 gcttcagcgc atgcggacga tgtttcgctt gtacaaaggg catgtggaca gactttatta
     1681 tggccggcaa tatttgacac gagagttttt tgatgacctg catcgccgtt tcgcgccgca
     1741 cctctgcctc atgcttgccg agcgcgaggg caaaattatt gcgggaacat tcaacgtacg
     1801 cgatgacacc gcgatgtacg gccggtattg gggctgtttc gaggaacatc cctttttgca
     1861 tttcaatgtg tgttactact ccgccatcga gcactcgatt cgcatcggat tggaacggtt
     1921 cgaggcgggc gctggaggaa gctttaaaca gcttcgtggt ttggaccctg agcataccac
     1981 cagcatgcac tacattgtcg acggcagatt gcgccgtgct gtggagcgct ttcttgccca
     2041 agagcgcgag atgatccaac gtaagcgtga gatgctgctc gaccacagcc aactgaaaca
     2101 cgagccgcag gagccgtaaa atcagtcggc gcatccgctg cttgcattcg cgcgaatgac
     2161 ccaattgtca tctcgaccgc gatagatctt gtattttcag ttgaatacga agaaaaattt
     2221 ctcgcgtttg gctcgagatg acagattacg cgatcgtctc gaaaccggca ggtcgggccc
     2281 gggaaatccc cacaaacact tccaaccgtt ttcttgacag catcgatagt ttcctctaac
     2341 gtcggcgcgg agtgcgcctg tgaaattttc gtggaagaat attgcggagc ccgtgctctt
     2401 tttcgcggtg cttttgctgc tctgggagat cctagttgag gtcattaaag ttccgacttt
     2461 tattctcccg ccgccgcgtg atctctggat cgcattttgg aaaaagctgc cgattctcgg
     2521 ccatcattca ttcattactt tcatcgaagc gttcggcggg ttcgtactct cgctcgttct
     2581 cggcgtcggg ttcgcgatcg cggtcgtgta ctcgcgccat ctgcagaata cgatttatcc
     2641 gctgatcgtg atcctttacg ccatgcccaa aagcgccttt gcgccattga tggtgatctg
     2701 ggtggggtac ggattgttct cgaagatcgc tatcgccttt ctcgtggctt ttttccctat
     2761 cgtcgtcaac accgtggtcg gtctgaagga aatcgagccg gagctgttgg agctggcgcg
     2821 aatcaatcgc gcctcggagt tcgacgtctt tcaaaagata cggctgccca actccattcc
     2881 ttatctgttt gccgggatca aagtggcgat cgtgttgtcg gtgaccggcg caattgtcgc
     2941 cgagttcgtg gctgctaacg aggggcttgg ctatttggtg cttcaggcga actacaatct
     3001 ggacacggcg tttgctctgg tgattttgct gattttggcg gtactgtccc ttggcctgtt
     3061 ctggctagcg gaagtcattc aacgcaaaat ggcgccgtgg agcgccggag tgcgcgaagt
     3121 cgaaggatct ttttaaacta ccggggaagg cggaagaatt catgtcactt attactggac
     3181 ggggaagcag catcattttc gctatcaccg ttgcccttct gctttttagt caagcgccac
     3241 tgcaagcggc cgaactgacg ccagccacga ttcggttgga ttttattatc ggcggaaagc
     3301 atgcgccgtg gtatgtcgcc ctggaaaaag gcttctacgc caagcgcggc ttgaatgcga
     3361 cgatacaatc gagcaccggc tcggccgaca cggtgcgaac gatcggctcc ggcggcgcag
     3421 attttggttt tgccgacatc gggacgacga tcgtcgccaa atcgcgcggc acgcctgtac
     3481 aagtcgtagg acagttcggc tatgtgcccg ccacgattct ctggcgtgat gaaggggtga
     3541 tcaagggcat tcaagatctc gccggaaaat cctgggcgat cagccccggc caagcccaat
     3601 ggtatctcat gccggcctac tgccgcatca acaagatcga ttttaaatcg atcaagatcc
     3661 aagaaaccgc agcgccgatt cagccggcag ctctcgcgac caagaaagcg gacttcattg
     3721 tgatgtatcg cgcctccaac gatgagatcg ctgaacaagc ggcgacgaag gtcggcgtca
     3781 agctctcgcg gattttcatg aaggataccg gcctagatat ttacggcaca ggtctgatcg
     3841 tgaaggatga cgacgtcaag aagcgtccgg atttcgtccg tgcgtacgtc gaagcgaccc
     3901 tggaaggtct tcgctatgcg cgcgataatc aggaagagtc gctcaaaatt ttgcttaaac
     3961 acaagcccga gctggagcca accttgacca gcttgcagtt aaaaaatgcc ttgaccgaag
     4021 tgtttttgcc gttcgagtcg gcgcaggtcg gtttaggtta catgaaaccg gaaatcatcg
     4081 agaaaaccgt caaggtgacc aacgagtatt tcgatgtggg ccgcaaagtt gccgccaaag
     4141 aagtcttcag caaccaattt atccgtcgat agcggatagt gcttccaagg tctatcagtg
     4201 ccgccgctga tcgccctcag tgacgtttcc cgcgttttta cgagcggcgg aacaaccgtg
     4261 acggcgctca atcgcgtggg cttcgacgtt caagcgggaa atttcgtttc catcgtcgga
     4321 ccgagcggtt gtggcaaaag cacgctgctc aaaatcgttg cgggtttgct gccctcgtcg
     4381 tcgggaaccg tcaccgtcca tggcgagcct gtgcgcggcc cgctggcaaa c
//
LOCUS       DASULZ010000839         5193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_97844, whole genome shotgun sequence.
ACCESSION   DASULZ010000839 DASULZ010000000
VERSION     DASULZ010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97844"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2416
                     /gene="clpB"
                     /locus_tag="VFY96_17230"
     CDS             <1..2416
                     /gene="clpB"
                     /locus_tag="VFY96_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEX5608272.1"
                     /translation="AKGGAQPGLSSRLASVAAAAEEEAERLKDEYTSTEHLFVAIVNE
                     GGRSASTRLLQQHKVSKVAVLQALTQIRGSHRVTDQNPEGKYQALERYGRDLTDAARK
                     GKLDPVIGRDDEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIVEGLALRMVNGDVPEGL
                     KDKRLIALDLGALVAGAKFRGEFEDRLKAVLKEVTEASGQIVLFIDELHTLVGAGAAE
                     GAMDASNMLKPALARGELRCVGATTLDEYRKRVEKDPALERRFQQVYVGEPSVEDTIA
                     ILRGLKERYEVHHGVRITDGAIISAATLSHRYISDRFLPDKAIDLIDEAGSRLRIEID
                     SMPIEIDEVERKILQLEIERQALKREEDKASRDRLAQLEREIENLRETSSALKAHWQN
                     EKKSIQQIRSLKEKIESTKVEEQQAERRGDLNRAAELRYGTLTQLQKQLEEANQKLAE
                     LQKNQKMLKEEVDAEDVAEVVAKWTGIPISKMLEGELQKLLKMEDRLKLRVIGQDSAI
                     HAVANAVRRARAGLQDQNRPIGSFIFLGPTGVGKTELCRALAEFLFDDEQAMVRLDMS
                     EFMEKHSVSRLIGAPPGYVGYEEGGYLTEAVRRRPYSVVLFDEIEKAHPDVFNALLQI
                     LEDGRMTDGQGRTVDFKNTVIIMTSNLGSQYILDLGANNRREMEQRVNAALREAFKPE
                     FLNRVDEIIVFNNLGREQIKSIVDIQLNRLRQNLASRKMFLEISERAKALLADKGYDP
                     VYGARPLKRTIQRLIQDPLAVKILAGDFKEGDRVHVDADAEEFTFERGDGAAADYGYE
                     QQPTLH"
     gene            2596..4038
                     /locus_tag="VFY96_17235"
     CDS             2596..4038
                     /locus_tag="VFY96_17235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5608273.1"
                     /translation="MMETAKITSSSQRRLKLILLNVCVGQFIVGLDQRALLVALPTLT
                     ETFNTSLTTIQWVLLIYDLLLIGTVITVGRLGDLFGRRRLYAGGFLIFVLSSALCGAS
                     QNTWQIILFRGLQAVGGAMISANGRAIASIAFPSQQRGQAMGFASMAFHVGFLTGPTL
                     GGFLIDTVGWRWIFYLNLPVGFWGAYLAWKLLEESRAESKEKISVDFRGAVLLMLTCS
                     LFIYAMNQLPHLGWAHPQVTTCLALAALALGLFIIVELKAPMPILSFSLFRIRLFTAS
                     MFSLFFITSTQSAISFLMPFYLQNVLHFSPTHMGWIIIANSAVIVMVAPVAGWLSDRM
                     GSRLLCTIGSSVIVIGQFFIASLAVDSSVPRILFPLLLIGLGWALFNSPNQSAILGSV
                     PHDKVGTASGMNTTTARTGGAMGVALSATLFTYGLAAAGLSRAEVESPQTWGNAPEVF
                     VHSFNHTIHIVNFFTILSVFFSAVRGSRQE"
     gene            4167..>5193
                     /locus_tag="VFY96_17240"
     CDS             4167..>5193
                     /locus_tag="VFY96_17240"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5608274.1"
                     /translation="MNFTKRELPIFLFAFLLAGVSATRTFSAVVPAAEEIWRSVEKLS
                     PDERNKKLIEGAKKEGEMLWYTNSGLENATRYIQAFKKNFPFINAQVWRAKTRQVTQR
                     VISEANAGRYLVDVVKPSTDLLPPLLDKNLLGKYETPIQSVYPAHAKSPYYTSMNYAF
                     RIFSFNPRKVNHKAAPKTWEELLQPRWRGEILFDESSLEEVMALLAAWGKEKTISYAN
                     RLSQQQLLIRVGRDTTMQMMMAGEAPLAVTTYAYNNEALRAKNAPVDWLAEDLIPTLL
                     YPLTMARNAPHPYAAALFYDFMLSEEGQRLIAKEGRVVGHPKVEPIYPRMKELQNLLG
                     TPRVQLNT"
BASE COUNT         1149 a         1481 c         1442 g         1121 t
ORIGIN      
        1 cgccaaaggc ggcgcacaac cgggactgtc gtcccgactg gccagcgtcg cggcggcggc
       61 ggaggaagaa gccgaacgcc tcaaggacga atacaccagc acggagcatc tcttcgtcgc
      121 catcgtcaac gaaggcgggc gttcagcgtc cacgcgcctg cttcaacagc acaaagtctc
      181 gaaggtcgcg gtcctgcagg cgctcactca aatcagaggt tcacatcgcg tgaccgacca
      241 gaatccagaa ggcaaatatc aggcgctcga acgctacggc cgggacctta ccgacgctgc
      301 gcgcaagggc aaactcgatc cggtgatcgg ccgcgatgac gaaattcgcc gggtcattca
      361 ggttttatcg cgccgcacca aaaacaatcc agtgctgatc ggcgagcccg gagtcggcaa
      421 aaccgccatc gtcgaaggat tggctttgcg gatggtcaat ggcgacgtgc cggaaggctt
      481 gaaggataag cgcctgatcg ctctcgattt aggagcccta gtcgccggcg ccaagttccg
      541 cggtgagttc gaagatcggt taaaagcggt tctgaaggaa gtcaccgagg ccagcggcca
      601 gatcgtgcta ttcatcgacg agctgcatac tttggtcggc gccggcgccg cggagggtgc
      661 catggacgcc tccaacatgc tgaagccggc gctcgcgcgc ggtgagctcc gttgtgtcgg
      721 cgcaacgacc ttggatgagt accgtaagcg agtcgaaaag gatccggcgc tcgaacggcg
      781 attccagcaa gtttacgtcg gcgagccgtc ggtggaggac accatcgcca ttctgcgcgg
      841 tttgaaggag cgttacgagg tgcatcatgg cgtccgcatt acggatggcg cgattatcag
      901 cgccgcgacg ttgtcacacc gctatatcag cgatcgcttc ttgccggaca aagccatcga
      961 tttgatcgac gaggcaggat cgcgtttgcg aatcgaaatc gacagcatgc cgatcgagat
     1021 cgatgaagtc gagcgaaaaa tccttcagct cgaaatcgag cgccaggcgc tcaaacgcga
     1081 ggaagacaag gcgtcgcgcg accgtctcgc gcaactggag cgcgagatcg agaacctgcg
     1141 cgagacctca agcgctctca aagcgcactg gcaaaacgag aaaaagtcga ttcaacaaat
     1201 ccgctcgctc aaggaaaaga tcgagtcgac caaggtggaa gagcagcagg cagagcggcg
     1261 cggcgattta aaccgcgcgg cggagctccg ctatggcact ctgacccaac tgcagaagca
     1321 actggaagag gcgaatcaaa aactcgccga gctgcagaaa aaccagaaga tgctgaagga
     1381 agaggtcgat gccgaagacg tggcggaagt cgtggcgaaa tggacgggaa ttccgatctc
     1441 gaagatgctc gagggtgagc tccagaagct gctgaaaatg gaagatcgcc tgaaactccg
     1501 cgtcatcggc caggatagcg ccattcatgc ggtggcgaac gcggtgcgcc gggcgcgcgc
     1561 tgggttgcag gaccagaacc ggcccatcgg ctcgtttatc tttctcggtc ctaccggagt
     1621 cggaaaaacc gagctctgcc gcgccttggc ggaattcttg ttcgacgatg aacaggccat
     1681 ggttcgcttg gacatgtccg agttcatgga aaagcactcc gtgtcgcgtt tgatcggcgc
     1741 tcctcccggt tacgtcggtt acgaggaagg cgggtatctg accgaagcgg tgcgccgccg
     1801 gccatactcg gtggtgctct ttgatgagat cgaaaaggcg catcccgacg ttttcaatgc
     1861 gctgttgcag attctcgagg atggccgcat gaccgatggc cagggccgca cggtcgattt
     1921 caagaacaca gtcatcatta tgacttcaaa cctcgggagc cagtacattc tcgatctcgg
     1981 cgccaataat cgccgagaaa tggaacaaag ggtcaatgca gcgctgcgcg aagccttcaa
     2041 accggaattc ttgaaccgtg tagatgaaat catcgtgttc aacaatctcg gccgcgagca
     2101 aataaaatcg attgtcgaca ttcaactcaa tcgcttgcgt caaaacctcg caagtcgaaa
     2161 aatgttcttg gagattagcg agcgcgccaa agcgttgttg gccgacaaag gctacgatcc
     2221 ggtttacggc gctcgccctc tcaagcgcac gattcagcga ttgatccagg atccactggc
     2281 ggttaagatt ctcgctggtg atttcaaaga aggcgatcgg gtgcatgtcg atgcagacgc
     2341 cgaggagttt acgttcgagc gtggcgatgg tgcagcggcc gattacggct acgagcaaca
     2401 accgacgctg cattgatcgc ggcgcagaca ggtgagcctc accgctaaaa atttctaagc
     2461 agcttagtcc taagtaatga gggggcagtc cacttcgatg ccccctcttt ttttgcattc
     2521 taaacggtcc ggcttcatcg attcatttcg ccggtctaat cgccagcaca caagtcacga
     2581 tgggtgcgcc ttcaaatgat ggaaacagcg aagataactt cctccagtca aagacggctc
     2641 aagctgattt tactcaatgt ttgcgtcggt caattcatcg tcggtctcga ccagcgggcg
     2701 ctccttgtcg ccttacccac actaaccgaa acctttaata ccagcctcac gaccattcaa
     2761 tgggtcctgt tgatctatga cttactcttg atcggcaccg tgatcacggt cggccgtttg
     2821 ggtgacctct tcggccggcg ccgattgtat gccgggggtt ttttgatctt cgtcttgagc
     2881 tccgcgctgt gcggcgcctc tcaaaacacg tggcagatta ttttgtttcg cggtttgcag
     2941 gcggtcggcg gtgcgatgat ttccgccaat ggccgagcga ttgcctcgat cgcgtttccc
     3001 tcccaacagc gtgggcaagc gatgggattt gcgtccatgg cgtttcacgt cggctttctc
     3061 acgggtccga ctcttggcgg ctttttgatc gatacggtcg gctggcgctg gatattttat
     3121 ctcaatttgc cggtaggctt ctggggcgcc tacctcgcat ggaaactgct ggaggaaagc
     3181 cgggcggagt ctaaggaaaa aatatcggtc gatttccggg gagcggtcct gctcatgctg
     3241 acctgcagcc tatttatcta tgcgatgaat caactgcctc atttaggttg ggcccatcct
     3301 caggtgacca catgccttgc cttggcggcc ctggcactcg gactgtttat cattgtcgag
     3361 ctgaaagcgc cgatgccgat cttgagcttt tcgttgtttc gcattcgcct cttcacggca
     3421 tcgatgttca gtttgttttt catcacctct acacagtcgg cgatcagctt cctgatgcca
     3481 ttctatctgc agaatgtcct ccatttctcg cccactcata tgggctggat tatcatcgct
     3541 aattctgccg tgatcgtcat ggtcgcgccg gttgcgggtt ggctctccga ccggatgggt
     3601 tcccgtctgc tctgcaccat cggctccagc gtgatcgtca ttggtcagtt tttcattgcg
     3661 tcgctcgccg ttgactcgtc ggtgccacga attttattcc cgctgctttt aatcggcctc
     3721 ggctgggcgc ttttcaactc gcccaatcag agcgccatcc tcggctcggt tccccacgac
     3781 aaggtcggca ccgcatccgg catgaacacg accaccgccc ggaccggcgg ggctatgggg
     3841 gtcgcacttt ctgcgacttt gttcacctac ggactcgccg cggcgggcct gtcccgcgca
     3901 gaagtcgaat cgccgcaaac ctggggcaat gcgccggaag tttttgtcca ctccttcaat
     3961 cacacgattc acatcgtcaa tttttttacg atactttcgg tgttcttttc ggccgtgaga
     4021 ggatcgcgcc aagaatgatt gcgaggtctg ctgcttggac cgacttcggc cggttaagcc
     4081 gcagcgccac tccagcttct cacgtttgac agcaaactca gccgtgccat agagtctgag
     4141 aagttgattt gagtctttgc cagctgatga attttaccaa gcgtgaactg ccgattttcc
     4201 tattcgcttt tttgctcgca ggcgtctccg cgacgagaac tttttccgcc gttgttccag
     4261 ccgccgaaga gatctggcgc tccgttgaaa aactcagccc agacgaacgc aacaagaagc
     4321 ttattgaagg cgcaaaaaaa gaaggcgaga tgctctggta caccaactcg gggctcgaga
     4381 acgcgactcg ctatattcag gcattcaaga aaaattttcc cttcatcaac gcgcaggtct
     4441 ggcgcgcaaa aacccggcag gttacgcagc gcgtgatttc ggaggcgaac gccggccgat
     4501 acctagtgga tgtcgtcaag ccatccaccg accttctgcc gccgctgctc gacaaaaacc
     4561 tgctcggcaa atatgaaacg cccatacaat cagtctatcc agcgcacgcg aaatccccgt
     4621 attacaccag catgaactat gcgtttcgca ttttcagttt taatccgcgc aaggtcaatc
     4681 acaaagccgc gccgaaaacc tgggaagaat tgctgcaacc gcgctggaga ggtgaaattc
     4741 tcttcgacga atcttctttg gaagaagtca tggctttgct cgccgcttgg ggcaaagaaa
     4801 aaaccataag ctacgcgaac cggctcagtc agcagcagct cttgatccgc gtcgggcgcg
     4861 atacgacgat gcagatgatg atggcggggg aagcaccgtt ggccgtaacc acttatgcct
     4921 ataacaacga agctttgcgc gcgaaaaacg cgcccgtcga ttggctcgcc gaagacctca
     4981 tccctaccct gctctatccg ttgacgatgg cacgcaatgc gccccacccc tacgctgccg
     5041 ccctattcta cgatttcatg ctctccgaag aaggccagcg actgattgcc aaagaaggtc
     5101 gggtcgtcgg ccaccccaag gtcgagccga tctatccacg tatgaaagag ctacagaatc
     5161 tgctgggcac gccgcgcgtc cagctcaaca ctc
//
LOCUS       DASULZ010000840         3332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1315230, whole genome shotgun sequence.
ACCESSION   DASULZ010000840 DASULZ010000000
VERSION     DASULZ010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315230"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="VFY96_17245"
     CDS             <1..962
                     /locus_tag="VFY96_17245"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5608275.1"
                     /translation="IESDVFEYLPEHYKKRREAVLYFPLLPHHGWHRQAGHGGTGSSF
                     LIPTLHDWQLALDQGNIEAAVVYPTRFMHIGQVGMIEFAVDLSRAYNDYLYDRFLRHE
                     SRLKGIAILPLQDVPAAMSELRRAVEEYGMVGAVLPADGLPRPLGHPEFHPVYEEANR
                     LGCMLAVHSQNSLRNNDLFMWRGEAATLAHVWPQMRQFTNFMFSGVLGKFPDLKIAFL
                     EAGCGWVPYLMSKMEARMGELPRPSQLLERRQIYFQCGEEMTTRRDLELLGDECLFWA
                     SDFPHEGIVDMKKAVDEFLSREDIPEASKRKISYDNPKRLYGL"
     gene            complement(1056..1436)
                     /locus_tag="VFY96_17250"
     CDS             complement(1056..1436)
                     /locus_tag="VFY96_17250"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5608276.1"
                     /translation="MGIEIVDLVAMAHKDKRRKNIFNTPRFHAWMHYYKPGQKDAMHC
                     HNADQTFVVLDGECTMSFPDGGKITLQPGQAALITGGSFYTLENTGEGPMIMMGNRSG
                     PAEKIQIIDYVTRKDIRQQAQPAD"
     gene            complement(1466..1846)
                     /locus_tag="VFY96_17255"
     CDS             complement(1466..1846)
                     /locus_tag="VFY96_17255"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5608277.1"
                     /translation="MAKRKSIHIKGMEHGAPIPNGVVIGNMVFSSAISGKDAKTGVLS
                     SDPDEQAEAMFRNLRLFMETAGGTPENIAYMKVYLKEEKLRDSVNKAWLKMFPDEHDR
                     PARHAIKTELRGDNLFQIEVIAVL"
     gene            complement(1906..2676)
                     /locus_tag="VFY96_17260"
     CDS             complement(1906..2676)
                     /locus_tag="VFY96_17260"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5608278.1"
                     /translation="MRLHYEVQGDGHPLVILHGFLGSSENWRAMRKLFATTYKVFSVD
                     QRNHGSSPHSSTMNYGVMTEDLRGFLSEQGLSKAFLLGHSMGGKVAMQFASQSPQAIE
                     KLVIVDVAPKAYPPAHRLLLQAMQNLELRGLKTYGEAEAALGATISDARLRKFVVKNL
                     TRDRNGEFQWRIGLDSLAANYDQLLKAPAMLNSFEKPTCFIRGGLSRFIDDQDFAAIR
                     KHFALAEFHTVQDAGHWVHIDAPEEFYRIVDEYLRLVG"
     gene            complement(2999..>3332)
                     /locus_tag="VFY96_17265"
     CDS             complement(2999..>3332)
                     /locus_tag="VFY96_17265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608279.1"
                     /translation="ELEKKLSASEQSVSELDQLRARSERDEREQQELREQIRQREHEL
                     VQWQSRVNEAEQHRERLAALQTPYHALISKQAALAEKQRELQADLEAFAKLMNHADSS
                     GTTSGAHR"
BASE COUNT          730 a          891 c          910 g          801 t
ORIGIN      
        1 aaattgaaag cgatgttttc gagtatctgc ccgagcacta caagaaacgg cgcgaagcag
       61 tgctgtactt tccgttgctg ccgcaccatg gatggcaccg gcaagccggg catggcggca
      121 cgggttcgtc gtttctgatt cccaccctgc acgattggca gctcgcgctc gaccaaggca
      181 atatcgaagc ggcggtggtc tatccgaccc gtttcatgca cattggccaa gtcggcatga
      241 tcgaatttgc cgtcgatctg agccgcgcct acaacgatta tctctacgat cgctttttgc
      301 ggcacgagtc gagactcaaa ggaatcgcga ttctcccttt acaagacgtg ccggcggcga
      361 tgagcgaatt gcgccgcgcc gtggaagaat acggtatggt cggcgccgtc ctgcctgccg
      421 acgggttgcc ccggccgttg ggacatcccg agtttcaccc ggtttatgaa gaagccaatc
      481 gactgggttg catgctcgcc gtgcattcac agaactcgtt gcgcaataac gatctcttca
      541 tgtggcgtgg cgaagccgcg accctcgcac atgtgtggcc gcaaatgcgc cagttcacga
      601 atttcatgtt ttccggcgtg ttgggcaaat ttccggacct caagatcgct ttcttggaag
      661 ccggctgcgg ttgggtcccc tacctgatga gcaaaatgga agcgcgcatg ggcgagttgc
      721 cgcgaccgtc tcaattgctc gagcgcaggc agatttattt tcagtgcggc gaggagatga
      781 ccacccgccg agacttggag ttgttaggcg acgaatgcct gttttgggct tcggactttc
      841 cccacgaggg gatcgtcgat atgaagaaag ccgtcgatga attcttaagc cgcgaagata
      901 ttcctgaagc gtcgaaacga aaaataagct acgacaatcc gaagcggcta tatgggttgt
      961 agaacgaccc gttcaagccg gcaaaacgtg ccaaccatat tgtcttcgat ttcaaccagt
     1021 tgaaccagtt tgaaccgttt gaacggatta aactttcaat cagccggctg cgcttgctgc
     1081 cgaatatctt tgcgcgtcac atagtcgatg atttgaattt tctccgccgg tccggagcgg
     1141 ttgcccatca tgatcatggg tccttcgccg gtgttctcga gggtgtaaaa cgatccgccg
     1201 gtgatcagcg ccgcctgccc cggctgcaaa gtgattttcc cgccatcggg gaaactcatc
     1261 gtacattcgc catccaaaac gacgaaggtc tgatcggcat tatggcagtg catggcgtct
     1321 ttctgccccg gtttgtagta atgcatccag gcatggaagc gcggcgtatt gaaaatattc
     1381 ttgcggcgtt tgtctttatg cgccatagcg acgagatcga cgatttcaat gcccattgaa
     1441 aaaacctcct cgttgtgagt tttcttcata aaaccgcaat cacttcaatt tgaaataaat
     1501 tatcgccgcg gagctccgtt ttgatggcgt gccgcgccgg gcgatcatgc tcgtcgggaa
     1561 acattttcag ccaggcttta ttgacggagt cacgcagctt ttcttctttt aaatagactt
     1621 tcatataggc gatattctcc ggtgtgccac cggcggtttc catgaacagc cgaaggttgc
     1681 gaaacatcgc ttcggcttgt tcgtccggat cggacgataa cacgccggtc ttggcgtcct
     1741 tgccggagat cgccgaagaa aaaaccatat tgccaataac aacaccgttg ggaatcggcg
     1801 cgccgtgttc catgcctttg atatggatac tcttgcgctt tgccattgct cgcctcccgc
     1861 ggattcaaac tttaggaatt catagcatga ggggcgctgg aaggtctacc ctacgagacg
     1921 gaggtattca tcgacgatgc gataaaattc ttcgggcgca tcgatgtgca cccaatggcc
     1981 ggcatcttga accgtatgga attccgcgag cgcaaagtgc ttgcgaatgg cggcaaaatc
     2041 ctgatcgtca ataaagcgcg aaaggccgcc gcgaatgaaa caggtcggtt tttcaaaact
     2101 gttcagcata gcgggcgctt taagtaactg atcgtaattc gcagctagcg aatccaggcc
     2161 gatgcgccac tgaaactcac cgttgcggtc tcgcgttaga ttcttgacca caaacttccg
     2221 gagccgcgca tcggaaatcg tcgcacccag cgcggcttca gcttcgccgt aggtttttag
     2281 cccgcgcaat tccaggttct gcatggcttg cagcagtagg cggtgcgcag gcgggtaagc
     2341 tttcggagca acatcgacaa tcacgagctt ctcaatcgct tgcggcgatt gggaggcgaa
     2401 ctgcatcgcc actttcccgc ccattgaatg gccgagcagg aaagcctttg aaagaccctg
     2461 ctcggataaa aatccgcgca ggtcctctgt catgacccca taattcatcg tgctgctgtg
     2521 gggcgaactc ccatggtttc gctgatcgac ggaaaagact ttatacgtag ttgcaaaaag
     2581 ctttcgcatc gcgcgccaat tctcggacga gccgagaaag ccgtgcagaa tgaccagtgg
     2641 gtgcccatcg ccctggactt cgtaatggag acgcatctgc aacccccggc atcagtatag
     2701 gaaatcaata gcgaaccgca taggagcgag cggctggcgc cgctctcgac gcttacgatt
     2761 gcagttcagt gccgcggatc agcgacgtgg tctagaacac ggtgctcgtg ggatcgccga
     2821 tcgggaaaga ctgtgaacgc agtttttttg aagcttgtag aatcgattgc ggcgggagat
     2881 tgtcggcgtt gtggacgtag aaacaaagca cacggaatgg agtgtgcctt gttcaagacc
     2941 aagggctgct aaagaatatt aaagtcggta ttttacctga ctccggtgtc gccttgcatt
     3001 atcgatgagc tccgctcgtc gtaccggagg aatcggcatg attcattaat ttcgcgaatg
     3061 cctcgagatc ggcttgcagc tcacgctgct tttctgccaa cgccgcctgc ttcgaaataa
     3121 gagcatgata cggtgtctgc agggcagcga gccgttcgcg atgttgttca gcttcgttaa
     3181 ctcgcgattg ccattgaacc aattcgtgct cgcgctgccg gatctgctcg cgcagttcct
     3241 gttgttctcg ctcgtctcgc tctgatcgag cgcggagttg gtccagctca gaaacgctct
     3301 gttcgcttgc gctgagtttt ttctcgagct cc
//
LOCUS       DASULZ010000841         8172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1517830, whole genome shotgun sequence.
ACCESSION   DASULZ010000841 DASULZ010000000
VERSION     DASULZ010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8172
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517830"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VFY96_17270"
     CDS             <1..766
                     /locus_tag="VFY96_17270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608280.1"
                     /translation="KCARTGCIVSKGSNARPAATVILLRPSAPTGFQVFLTRRPEGMR
                     FLGGMYCFPGGSVNQEDSSPRMMARCSGVSPQQARKIIGAQFRPQAALGFWIAAIREL
                     FEETGIFLAVRDSGTPGSIEAMQSHRLVEKHAVLIAKSLSFCELLESENLYCNASALA
                     YFSHWQTPAQNALRFDTRFFLTVLPQAQTPLPSSYEVIHSLWLTPDRAIQLFERGELP
                     LIFPTFASLRTLADFDTLESLFEELSHGSAVASGAR"
     gene            903..2147
                     /locus_tag="VFY96_17275"
     CDS             903..2147
                     /locus_tag="VFY96_17275"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5608281.1"
                     /translation="MADLYYGWRMIAVGSMLRILGGGLYFYGFSVFFLPLSQDLSLSR
                     ASTSLVFSLARAQGAFEGPIAGYFMDRHGPRPLMIMALMMTGIGHMLLSGVHSYVTLL
                     IVYMGVVSLSFHAGFMDAPMLIANTWFIRKRTMAMALISGSIGIGGFIFTPVLSAVVH
                     AFGWRQAAFACGIVFIVVGMPLALLVRRSPESIGLLPDGDRVLPGDTGQTQDGRATEE
                     INFTLAQALRTSSFWFIVSATAIRVIILSAINVHYVALMVWKGVSEQRAAYFLAAQAF
                     MSVPSHLLFGWIGDLLPKPKLMATCMLLALVSMCVLARVHSEWAILIFIALFSVVEST
                     FPVNWSTVGEYFGRKNFAKIRGSMSFVSTWGSVIGPVIAGAIYDHTRSYELVIWSSAA
                     LLLVASLLYASVKKPLPPTEAS"
     gene            complement(2191..3141)
                     /locus_tag="VFY96_17280"
     CDS             complement(2191..3141)
                     /locus_tag="VFY96_17280"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5608282.1"
                     /translation="MKFRVAAVDLISNTCFPALAADELGYFKAEGLEASIELFPMLNA
                     TKALHDGNADAMIAGSVHDVLTEFPNWDGVKIIVALSQGTPWLLTVRSDLPCKRGDLN
                     SVRGLRLTAAQGPDLALKRMLQIAGIDPARDVEIVELPNAKSRNVSFGVFAAQALQSG
                     QIDGFWANAMGAETAVNHGVGKVLIDVRRGDDPDGVRFFTFAGVATTDDFIQREPKTI
                     AAAVRAIVKAQTALRAEPSLAREVGARKFPADAAQLIANVVARDVEFYDPVITEGAIA
                     KMNAFAQSVGHLSQPVTYERVVAVQYRSLWQDPTSGQSGI"
     gene            complement(3226..5142)
                     /locus_tag="VFY96_17285"
     CDS             complement(3226..5142)
                     /locus_tag="VFY96_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5608283.1"
                     /translation="MPILRLENLSLAYGHLPLLSQVEFQVELGERVCVVGRNGAGKTT
                     LFRVIMGTASADEGEIWRADTLRVAHLEQEVPPDTEQTIFEVVAAGLGELGSVLSQYH
                     RLSHGAGIEKRDSLDQLAHLQSRIESLGGWNVNQKVESVLTRLSLPDSVRLSDCSGGT
                     RRQVMLARALVRDPDLLLLDEPTNHLDINAITWLEKYLQNYRGALMFITHDRTFVRRL
                     ATRIVELDRGKLTSFPGDFDAYLRKKDELLDIEERANARFDKQLAEEEAWIRRGIKAR
                     RTRNEGRVRALQVMRREKAERLEAQEKARFGIDSGALSGRLVVDVRSVSFGFGENVII
                     RDLSTRILRGDRVGIIGPNGSGKSTLLRLVLGELKPDRGEVVLGTQLQVAYFDQHRRT
                     LEPNKTVRENISDSDYVSVRGRSRHVIGYLKDFLFPPARIDSPVKALSGGERNRLLLA
                     KIFTQSANMMVLDEPTNDLDVDTLELLEELLAAYEGTLLLVSHDRTFLDNVVTSTLVF
                     EGEGKFLEYAGGYEDWERYQRQIPAANSRAQRSATPTAVAVPVKSRSAEKRRRLSYKE
                     QRELDQLPAKIEALEAEQVELHACMSTGDFYRQSSDKIAAAVERLEALRRELEEHYQR
                     WQTLESITGEAAVD"
     gene            complement(5199..5492)
                     /locus_tag="VFY96_17290"
     CDS             complement(5199..5492)
                     /locus_tag="VFY96_17290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608284.1"
                     /translation="MKDLIQKLGPILVGSATVVANLRKLLAESRPNGTDARIDAVENA
                     LQIQSTLNESLDVQVRLIHELLKKTQKRLQIVIVALIATGTVAALALAVAAMK"
     gene            complement(5685..6824)
                     /locus_tag="VFY96_17295"
     CDS             complement(5685..6824)
                     /locus_tag="VFY96_17295"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5608285.1"
                     /translation="MKNRAFLFLCITLLNGLGCSLAPNNFGSDSGSARLSRRSRGPDF
                     VTLVKQLQPAVVNVSVTMLPVPPSTHGVQPQGSFNGNFGEFFGAPPTVPPSPQRQQGS
                     GFIIGTEGIILTNAHVVTGAKKIIVKLGDKREFEARVIGADAPTDVAMLKIDVKDSLP
                     TIRLGDSDNLEVGEWVMAVGTPFGLDNSVSSGIVSGKGRHIGESYDRLIQTDATLNPG
                     SSGGPLINLNGQVVGINKAIVSETGGSIGISFATPINLIKEILPQLQSKGKVTRGWAG
                     ISIQELNSALAETLGLEKPTGALVAGLAKDGPADRGGIKLGDIILEYDGMPVTDALDL
                     PLWIARTTIAKRVGVKIQRDGEEMSLDLTVAELPETQLRVGNQIG"
     gene            7110..7403
                     /locus_tag="VFY96_17300"
     CDS             7110..7403
                     /locus_tag="VFY96_17300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608286.1"
                     /translation="MKSAKNIIAGLALITGLIGLAAPASAAWSHDSDFSRDRRELATA
                     RQELRHDLRRGAGHREIARDRAAIARERREIWADRRDWRNDRGRRDYRPWFRW"
     gene            7645..>8172
                     /locus_tag="VFY96_17305"
     CDS             7645..>8172
                     /locus_tag="VFY96_17305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608287.1"
                     /translation="MAFPSFDTATNSWAPQADISWPIGPERQSAFVRFPLRVMTESEA
                     VACALRRSQSWIDNRLKARSAENAATLVIDRINPLPNSFRRTNLKPPAKPKPTIRNDS
                     TESFTFAQFKSALMRPGTKVADSVLRKSYSGLMQLQKNKNWSTAEIRAMVNRVQELLL
                     FQSKRRGQRCRLPMTE"
BASE COUNT         1719 a         2322 c         2201 g         1930 t
ORIGIN      
        1 gaaatgcgct cgcactggat gcatcgtgag taaaggatcc aacgcacggc cggctgccac
       61 agttattctt ctgcgcccgt cggcgccaac gggcttccaa gtctttctga cgcgccgccc
      121 cgagggcatg cgttttttgg gcggcatgta ttgttttccc ggcggtagcg taaaccaaga
      181 agattcttcg cctcggatga tggcgcggtg ctcgggtgtg agtccgcagc aagcgcgcaa
      241 aatcattggc gcgcaatttc gaccgcaagc cgctctcgga ttctggatcg ctgccattcg
      301 cgagctattt gaagagacgg ggattttttt ggccgtgcgg gattcgggaa cacccggatc
      361 gatcgaggcg atgcaaagcc atcggctcgt ggaaaagcat gccgtcctca ttgcgaaatc
      421 actttctttc tgtgagctgc ttgagagcga aaatctttac tgcaatgcgt cggctcttgc
      481 ctacttttcc cattggcaaa cgccagcgca aaacgccctc cgttttgata cgcgtttttt
      541 tctcacggta ttaccacagg cgcaaacgcc cttgccaagc tcctatgagg tcatccatag
      601 cctttggctc acccccgacc gggcaattca actgttcgag cgcggcgagt tgccgctgat
      661 ttttccgact tttgcgtcgc ttcgcaccct tgctgatttt gataccttgg aaagcctgtt
      721 cgaagagttg agtcacggaa gcgctgtcgc atcaggcgca cgttgactaa acttctgcct
      781 cgcgttgctt tagcgcggtc acttcgttaa ttttaacatt ccatccgcag gatcccgccg
      841 cgggactccg gttgcgacaa acgcgaatag gcagctacgt gcttcactcc ttacgaggcc
      901 gattggccga tctttactat ggctggcgca tgatcgccgt cggctcgatg ctacggattc
      961 tcggcggcgg tctctacttt tacggcttct cggttttttt tctgccgctg agtcaggatt
     1021 tgagtctcag tcgtgcatcg acgtcactgg tcttttcttt ggcgcgcgca caaggcgcgt
     1081 tcgaaggccc gattgccggc tattttatgg atcgacatgg gccgcgcccg ctgatgatta
     1141 tggctctgat gatgaccggt atcggccaca tgcttctatc aggagtgcac agttacgtca
     1201 cgctgctgat cgtttatatg ggcgtggtat cgctttcgtt tcacgccggc ttcatggacg
     1261 caccgatgct tatcgccaac acgtggttca tccgcaagcg gaccatggcg atggccttaa
     1321 tcagcggctc catcggcatc ggtggcttta tttttacacc cgttttgtcc gcggtcgttc
     1381 atgcctttgg ttggcgtcag gccgcattcg cctgcggcat tgtctttatc gtggtgggaa
     1441 tgccattagc gctcctggtt cgccgttcac cagaaagcat cggcctactt ccagacggtg
     1501 atcgcgtcct acccggtgat accggccaga cgcaggatgg gcgcgccact gaagagatca
     1561 actttacgct cgcacaggcg ctgcgcacct cttcgttttg gtttatcgtc agtgcgaccg
     1621 cgatacgggt aattattttg agcgcgatca acgtccacta cgtcgcgctg atggtttgga
     1681 aaggtgtgag cgagcaacgt gccgcgtatt ttctcgcggc tcaggcgttc atgtcagttc
     1741 cctctcacct gctcttcggc tggatcggcg atcttcttcc caaacccaaa ttgatggcga
     1801 cttgcatgct tctggcctta gtatcgatgt gtgtccttgc gcgcgtacat tccgaatggg
     1861 cgattttgat ttttatcgcg cttttcagcg tcgtcgagtc gacattcccg gtcaattggt
     1921 cgaccgtcgg cgaatatttc gggcgaaaaa acttcgccaa gatacgcggc tcgatgagtt
     1981 tcgtctccac ctggggaagc gtcatcggtc cggtgatcgc cggagcaatt tacgatcaca
     2041 cacgaagcta cgagcttgtg atctggtctt ccgccgctct gttgctggtg gcaagcctgc
     2101 tctatgcgtc cgtcaaaaaa ccgttaccgc ccaccgaggc gagctagttt cgggctctag
     2161 taaataaact cgaacagttg gcacaagcct tcatatcccg gattgaccgc tggtcggatc
     2221 ttgccacaag ctccggtatt gaacggcaac aacccgttcg taggtcacgg gttgcgatag
     2281 atggccgacg gattgggcga acgcgttcat tttcgcaata gcgccttcgg tgatcaccgg
     2341 atcgtaaaat tcaacatctc gcgccacgac gttggcaatc aactgagcgg cgtcggccgg
     2401 gaacttccgc gcgccgactt cacgagcaag cgaaggctcg gcacgcaacg ctgtttgcgc
     2461 tttgacgatc gcgcgcacgg ctgccgcaat agtttttggc tcccgttgga tgaagtcatc
     2521 ggttgtggcc actccggcaa aggtgaaaaa gcgcaccccg tcgggatcgt cgccgcgccg
     2581 gacatcgatc agaacctttc ccactccatg atttactgcg gtctccgctc ccatggcgtt
     2641 ggcccaaaat ccgtcaattt gtccggattg caatgcttgc gcggcgaata cgccgaaaga
     2701 gacattccgg gatttagcgt tgggcagctc gacaatttcg acgtcgcgcg cgggatcgat
     2761 accggcaatc tgcagcatgc gcttgagcgc aaggtccggc ccctgggcag cagtcaagcg
     2821 tagcccgcga accgagttca aatctccgcg cttgcacggc agatctgatc tcacagtcaa
     2881 aagccatggc gttccttgcg acaaagcgac aatgatcttc acaccgtccc aattaggaaa
     2941 ctcggtcaaa acgtcgtgca ccgaaccggc gatcatggcg tcggcattgc catcatgcaa
     3001 cgctttcgtt gcattcagca tcggaaaaag ctcgatgctc gcctccaagc cttccgcctt
     3061 gaaataaccc agctcgtccg ccgccagagc cggaaaacag gtattgctaa tcaggtcgac
     3121 cgctgcaacg cgaaacttca cctagtcctc cgttcagttg gaattactgg gctattgcga
     3181 gctagtacgc tagcgcctgt tggcgaccta ctcgaatcta tgatttcagt cgaccgccgc
     3241 ttctccagta atcgattcca gggtctgcca acgctgataa tgctcttcca gctctcgtct
     3301 aagcgcttcg aggcgttcta cggcggcggc aattttgtcg ctcgattgcc gataaaaatc
     3361 gccggtgctc atgcacgcat gcagctcaac ctgctcagcc tccaaagctt cgattttcgc
     3421 gggtaactgg tcgagttcgc gctgttcctt gtagctgagc ctacggcgct tctcggcact
     3481 cctggatttg actggtaccg cgacggcggt gggtgtggca ctgcgctgag cccttgagtt
     3541 cgctgccggg atttgccgct gataacgctc ccagtcttca tagccgcccg catattcaag
     3601 aaacttgccc tcgccttcaa agaccaacgt gctcgtaacg acattatcga gaaacgtgcg
     3661 atcatggctc accagcaaaa gcgtaccctc ataggcggcc agcagctctt ccaagagctc
     3721 cagagtgtcg acatccaaat cattggtcgg ctcatccagc accatcatat tcgccgattg
     3781 ggtaaagatt ttggccagga gtaaccgatt gcgctcaccg ccagaaagcg ccttgacggg
     3841 cgaatcgatt cgcgctggag gaaagagaaa atccttcagg tagccgatca catggcgtga
     3901 gcgacctcta acgctcacat agtctgagtc gctgatgttt tcccgcacgg ttttattggg
     3961 ctccagagtt cgtcggtgct gatcgaagta agcgacctgc agctgggtcc cgagcacgac
     4021 ttcaccccgg tcgggcttca gttcgccgag cacgagccgc agaagcgtgc tcttgccgga
     4081 gccattgggc ccgataattc cgacccgatc tccgcgcaaa atacgagtcg aaagatcgcg
     4141 aataatgacg ttttccccaa acccaaaact cacgctccgg acgtcgacga caagccttcc
     4201 ggaaagggcg cccgaatcaa tcccgaagcg cgctttttcc tgagcttcga gccgctccgc
     4261 cttttcacgc cgcatgactt gcagtgctct gacccgtcct tcgttgcgag ttcgccgtgc
     4321 tttgataccg cgccgaatcc aggcttcttc ctcggcgagc tgtttatcga atcttgcatt
     4381 agcgcgttct tcgatatcca gcagctcgtc tttcttgcgc aggtaggcat caaaatcccc
     4441 cggaaatgaa gtcagtttgc cgcggtccag ctcgacgatg cgcgtcgcca ggcggcgcac
     4501 aaatgtgcgg tcatgtgtga tgaacatcag ggcgccgcga tagttctgca gatatttctc
     4561 gagccaggtg atggcgttga tgtccagatg attggttggc tcatcgagca agaggaggtc
     4621 gggatctctg accaacgcgc gggcgagcat cacctggcgg cgggttccac cggagcaatc
     4681 tgaaagtcta acagaatcgg gcaggctcaa gcgggtgaga acgctttcga ctttttggtt
     4741 tacgttccag ccgccaaggc tttcgatccg actctgcagg tgggccagtt ggtcaagcga
     4801 gtcgcgcttt tcaataccag cgccgtgact gagacggtgg tattggctga ggaccgagcc
     4861 caactctccg aggcccgctg ccactacctc gaaaatcgtc tgttccgtgt ccggcggcac
     4921 ttcctgttcc aggtgagcaa cgcgaagggt gtcagcgcgc cagatctccc cttcatccgc
     4981 gctggcggtt cccataatga cacgaaaaag agtagttttt ccggcgccgt ttcgaccgac
     5041 gacgcaaacg cgttcgccaa gctcgacttg aaactcgacc tgagacagga gcggcaagtg
     5101 cccataagcc agggaaagat tttctaagcg aagaatcggc atgatgaggt cagaacagga
     5161 tcgagatcgg cttggaaagc aatttcgcgg tggggaggct acttcatcgc tgcgacggcg
     5221 agcgcaagag ctgcgacagt tcccgttgcg atcagcgcca caataacgat ttgcagccgc
     5281 ttctgggttt ttttcagcag ctcatgaatc aggcgcacct gaacatccag gctttcgttg
     5341 agggtggatt gtatttgcag cgcgttttca accgcgtcaa tacgcgcatc tgtgccgttc
     5401 ggacgagatt cggcgagcaa cttgcggaga ttggcaacta cagttgcgct tccaacaagg
     5461 attggaccca acttctggat gaggtctttc ataaaagagg acaagccaat tacgcctaca
     5521 tcataccgaa gtctggcgct gcgacaagac cgctgtgggc gagcaaatgg tgcgcagcga
     5581 cgaatgggtg taccgctaag ccagtccgat ggtttacgat ggtaatacgc aaacgctaag
     5641 ctcgcccgct ttcctcgccg gcgtttcgcg atgaaaagac ataattaacc gatttgattt
     5701 ccgactctga gttgagtctc aggcaattca gcaaccgtca ggtccagact catttcttct
     5761 ccatcgcgct gaattttgac tccgacccgt ttcgcgattg tcgtgcgcgc gatccacagc
     5821 ggcagatcca aagcatcagt caccggcatt ccgtcgtatt ctaaaataat atcgccgagt
     5881 ttgatcccgc cacggtctgc tggcccgtct ttggccagac cggcaacgag ggcgccggtg
     5941 ggtttttcca gaccgagcgt ctctgcgagg gccgagttca attcctggat cgaaattcct
     6001 gcccagccgc gcgtaacttt tcctttgctc tgaagctgcg gtaaaatctc cttgatcaaa
     6061 ttgattgggg ttgcgaaact gatcccaatg ctgccgccgg tttcgctgac gattgcttta
     6121 ttgataccga ccacttggcc gttgaggttg attaacggtc cacctgagct tccaggattg
     6181 agagttgcat ccgtttgaat gaggcgatcg taggattcgc cgatgtggcg acctttgcca
     6241 ctgacgattc cagaactgac gctgttatct agcccaaagg gagttcctac agccatgacc
     6301 cattcgccga cttcgagatt atccgaatcg cctaatcgaa tcgtcggcaa actgtctttg
     6361 acgtcgatct taagcatcgc gacatcagtc ggcgcgtctg cgccaatcac acgggcctcg
     6421 aattcccgtt tgtccccaag cttaacgata atttttttcg ctccggtgac cacgtgggcg
     6481 ttggtgagaa ttataccctc ggtgccaatg atgaaccccg acccttgctg tcgctgcggc
     6541 gaaggcggca ccgtcggcgg cgcgccaaag aattcgccaa agttgccgtt aaaacttccc
     6601 tggggctgaa caccgtgcgt actcggcggt acaggcaaca tcgtcaccga cacgttcact
     6661 accgcaggct gtaattgctt caccagcgtc acaaaatccg ggcctctgct gcggcgggaa
     6721 agtcgcgccg aaccgctgtc gctgccgaaa ttgtttggtg cgagactgca accgagcccg
     6781 ttcaataagg taatgcacag aaacaaaaaa gcacgattct tcatcgattc ctccgcaact
     6841 tagaagcggg tcgagtttga gcctcgattc gctcagggcg tgcagtattt cccgcaccct
     6901 tcgcgcaacg tctatgccac aacaacgaag atgagatccg cgtcataact cgatatttcg
     6961 aacatcaacg agctgcgcgc ggttcaatgc atgattttca cggactggct tgtcggaatt
     7021 cgccgagaat tcgcataaaa gtggagccgc attggcaggt gcaacctttc caaggccaaa
     7081 acgtcaaagg gttagaaacg gaggtaacaa tgaaatctgc taaaaacatt atcgctggac
     7141 tcgcgttgat cacgggtctg attggactcg ccgcgccagc atcggcggca tggtctcacg
     7201 acagcgactt ttcacgggat cgtagagaac tagctacggc gaggcaggag ctgcgtcacg
     7261 atctgcgtcg cggcgccggg catcgcgaga tcgctcgaga tcgcgcggcc atcgctcgtg
     7321 aacggcgcga aatctgggcg gacaggagag attggcgaaa tgaccgcggc cgccgcgatt
     7381 atcggccctg gtttcgctgg taaccccgct caagtaactt aacgaagctc tttcggtccg
     7441 tctcattcgg tgggacgggc ctttttaccc gttttcaggc gtcgagatat ttttcttgat
     7501 gccttgtttt tttctgtcga cctgccaaaa ttgggcggaa ggacacagct ccttcggggt
     7561 aggcgctagc agctcaggca actcttcata ccaccaggag aggagttaat catgtggagt
     7621 cgttcctacc ggagacatgt catcatggcg ttcccgagtt tcgatacggc gacaaactcc
     7681 tgggcacccc aagcagatat tagctggcca atcgggccgg agcggcaatc ggcgttcgtg
     7741 cgctttccac ttcgggtgat gactgaaagt gaagccgttg cttgcgctct acgaagaagc
     7801 caatcatgga ttgataatcg tttgaaggcg cgcagcgctg aaaatgcagc gaccttggtg
     7861 atcgaccgca ttaatccttt gcctaattca ttccggagaa ctaatctcaa gccgcctgcc
     7921 aaacctaagc cgacgatccg gaatgattcg actgaatctt ttactttcgc acaattcaaa
     7981 tccgcgctca tgaggcccgg caccaaagtt gccgatagtg tattacgcaa aagctacagt
     8041 ggactcatgc agttgcagaa aaataagaat tggtcgacgg cggaaattcg ggcgatggtc
     8101 aaccgtgttc aggagctctt gttattccaa tccaaacgcc gtgggcaacg ctgtcgctta
     8161 cccatgacgg aa
//
LOCUS       DASULZ010000842         2695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_706513, whole genome shotgun sequence.
ACCESSION   DASULZ010000842 DASULZ010000000
VERSION     DASULZ010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706513"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VFY96_17310"
     CDS             <1..474
                     /locus_tag="VFY96_17310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5608288.1"
                     /translation="LLDGELEAALYPEILPSVRGRDPRVALLFRNPKQAEIEYYKNGG
                     FFPIMHTVVIKNAVLEKNPWTAISLVRAFQRAKEICYARNSDPRSFALVWVQDLLREQ
                     REIFGPDPWPYNLDDNRKALEAVIRYESEQGMIRKRPQPEELFFPPSLQTIQQYV"
     gene            complement(507..1304)
                     /locus_tag="VFY96_17315"
     CDS             complement(507..1304)
                     /locus_tag="VFY96_17315"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX5608289.1"
                     /translation="MGTQLFFYDFRRPSLPPTSLAQPSIGALAGNGLVSTPLEQPDGT
                     ADFRRTDPEVAVFDTSASFAWLNIVNRKLDLLWVAISIGLALFTWHYAVREAYAPIWL
                     YVALNIQCAVLFALRHPAQLTTRDPLEVGVTLVSLNYIFAFQPLPIASSAWAPLGGVI
                     CTAGAFLTLISVHSLGRSFAVLPSLRPIQTSGTYRFVRHPIYFSYLVTALGIIIRHPS
                     IYNGGVALVGVMLVLWRIKFEERLLAQEPSYRDYVARVRYQLIPGLY"
     gene            complement(1343..1603)
                     /locus_tag="VFY96_17320"
     CDS             complement(1343..1603)
                     /locus_tag="VFY96_17320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608290.1"
                     /translation="MEISLKVGSREYDQLRTHVPDGSTAREAVERATRIDYSVGGVLF
                     EGYTIVCDEDAARGLQETAAHYCPEIVAKIQEAVRLARSGQT"
     gene            1764..2051
                     /locus_tag="VFY96_17325"
     CDS             1764..2051
                     /locus_tag="VFY96_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608291.1"
                     /translation="MIRRSTVMLVALFAIGGCTSGSYSVANRPQEDLGGGYCHKQLSP
                     VGPSDLARSTQPNGDYIDYYGSCDGPSTAEQVQKQKRFEQFRFGKEYMDEG"
     gene            complement(2227..>2695)
                     /locus_tag="VFY96_17330"
     CDS             complement(2227..>2695)
                     /locus_tag="VFY96_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018110510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEX5608292.1"
                     /translation="FGEIQRQIVANVARPLRVVLYRRMMLRIGEAIARKEKAKAVVTG
                     ESLGQVASQTLENMAVIQQAARLPILRPLVGMDKQEIIDQARRIGTFEISSIPDQDCC
                     QLFVPAHPATKAHFAAVEEDETKLAIPELVRYGLENAIEEEFNFPESCPRSAD"
BASE COUNT          653 a          719 c          774 g          549 t
ORIGIN      
        1 ttgctcgatg gcgaactgga agcggcgctt tacccggaga tcctgccatc ggttcgcggc
       61 cgcgacccca gagtagcttt gctgtttcgg aatcccaagc aggcggagat cgagtattac
      121 aaaaacggcg gtttctttcc gattatgcac accgtggtca tcaagaacgc ggttctggag
      181 aaaaatccgt ggacggcgat tagcttggta cgagcgtttc agagggcaaa agagatttgc
      241 tacgcgcgca acagcgaccc gcgaagtttc gctttggtgt gggtgcagga cttactgcgc
      301 gagcaacgcg agattttcgg cccggatcca tggccctaca atctcgacga caatcgcaag
      361 gctttggaag cagtgatccg ctatgagtcc gagcagggca tgatcagaaa aagaccgcaa
      421 ccggaggagt tattctttcc gccgagcctg caaaccattc agcagtacgt ttgagcgcgg
      481 cagcgaatct gtaacgtcgg cgttattcaa tacaatcccg ggatcagctg gtagcgtacc
      541 cgcgcgacat agtcgcgata actcggctcc tgtgcgagca ggcgctcttc gaatttgatc
      601 cgccagagaa ccagcatcac acccacaagc gcgacgccgc cgttgtaaat actcgggtgg
      661 cgaataatta ttcccagcgc ggtgacaaga tatgagaaat agattggatg gcgcacaaag
      721 cggtacgttc cagaggtctg aatcgggcgc agcgacggca gaacggcaaa gctacggccc
      781 aacgagtgga ccgagatcaa tgtcagaaac gctcccgcgg tgcatattac gccgccaagc
      841 ggagcccatg ccgaggaagc aatcggtagg ggctgaaacg caaagatata gttcagcgag
      901 acgagagtta caccgacttc taagggatcg cgggttgtaa gctgcgccgg gtggcgtagc
      961 gcaaacagaa cggcacactg aatattgagc gcaacataga gccagatcgg cgcgtaagcc
     1021 tcacgcactg cgtaatgcca cgtgaagagc gcaagtccga tggagatcgc tacccaaagc
     1081 agatccaatt ttcggtttac gatgttgagc caggcgaatg atgcggaagt gtcaaaaacg
     1141 gcgacttcgg gatcggttcg gcggaaatca gcggtaccgt cgggttgctc taaaggcgtt
     1201 gaaaccagac cgttgccggc aagggctccg atcgacggtt gtgcgagaga cgtgggcggg
     1261 agggaaggtc gcctgaagtc gtaaaaaaag agttgagttc ccatcggctt tcccaagcag
     1321 gtctcacggc cgggcaaata acttaggtct ggcccgacct tgcaagacgg actgcttctt
     1381 ggatcttcgc tacaatttcc gggcaataat gtgccgcggt ttcctgcaaa ccgcgagctg
     1441 catcctcatc acaaacgatc gtgtaacctt cgaacaaaac accgcctacc gaatagtcga
     1501 ttctcgttgc cctttcaacc gcttcgcggg cggtcgagcc atcgggaaca tgggtgcgca
     1561 gctggtcgta ctctcgggaa ccgactttaa gcgatatctc cacaagccct cctcggcaag
     1621 gtaacgcaat cagaaagcgc cggcagatgc aagcaggcag ggttggagcc attttaattg
     1681 ccaggataac aggtacaatg attctccggc tcagggctcg gggtatcggt tcacaaacct
     1741 cggcaaaaag aaagggatta cctatgattc gtcgttcaac agtgatgctg gtggctttat
     1801 ttgcgatcgg cggttgtacg agcggctcgt acagcgtggc aaaccgccct caggaagacc
     1861 tcggcggtgg ctattgtcat aagcaattgt cacctgtcgg tccttcggat ctggcgcgct
     1921 cgacgcaacc caacggagat tacatcgact actacggttc atgcgacggt ccgtcgaccg
     1981 ctgagcaggt acaaaagcaa aaacgcttcg agcaattccg gttcggcaaa gaatatatgg
     2041 acgaaggctg agctgaacag gccgggcagt ccattcaaaa catggaagca ccgcagagac
     2101 acagagtaca cgaactttga ttctgtaacg gacttagcga aactcgggtc gtctcgagcc
     2161 tgtggtgaac tcctcccgag ccgcacgcaa gccgcggtgg ttcacctggc cgcgcggctg
     2221 cgcggttcaa tccgctgaac gtgggcagct ttccggaaaa ttaaattcct cctcgatagc
     2281 attttcgaga ccgtaacgga ccaattccgg gattgcgagt ttagtttcgt cctcttcgac
     2341 tgcagcaaag tgtgccttgg tggctggatg cgcgggaaca aaaagctggc agcaatcctg
     2401 gtccggaatc gaggaaatct cgaatgtgcc gatacgtctg gcctgatcga taatctcttg
     2461 tttgtccatg ccaaccaagg gtcgcaggat cggcaggcgc gccgcctgct gaatgaccgc
     2521 catattctcc aaagtttgtg aagccacctg accaagactc tcgccggtca cgacggcttt
     2581 ggctttttct tttcgtgcga tcgcttcacc gatgcgcagc atcatacggc ggtaaagcac
     2641 cacccgtaag ggtcgcgcga cgttggcaac aatctggcgt tggatctcgc cgaag
//
LOCUS       DASULZ010000843         2435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_198150, whole genome shotgun sequence.
ACCESSION   DASULZ010000843 DASULZ010000000
VERSION     DASULZ010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198150"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..123
                     /locus_tag="VFY96_17335"
     CDS             <1..123
                     /locus_tag="VFY96_17335"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF014716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX5608293.1"
                     /translation="KTSLNPLREDRVTNRPDTESLLANAPDRDGTFFKVPKIIE"
     gene            120..1589
                     /gene="gatA"
                     /locus_tag="VFY96_17340"
     CDS             120..1589
                     /gene="gatA"
                     /locus_tag="VFY96_17340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX5608294.1"
                     /translation="MSFAELTLHDAADKLRKREFTAVELTEAVYERIAETEPRVCAYL
                     TLAHDSALEDAARADEKRKNTASGAPLLGIPLAIKDNFLTKGVRTTCASKILGDFLSP
                     YDATVISKIRSAGAVIIGKTNLDEFAMGSSAENSAFFPTHNPWNLERIPGGSSGGSAA
                     AVAADECTAALGTDTGGSIRQPAACCGVVGLKPTYGRVSRFGIIAFASSMDQVGPMTK
                     DVRDSALLLEAIAGYDPADSTSVNSTVPRYSEMLAGDIKRIRVGVPKEYFASGVAAEV
                     KDAVRKAIGELEKQGAVIEEISLPSTEYAIAVYYIVATAEASSNLARYDGMRYGHRAN
                     AGDLLDTYTLSRAAGFGAEVKRRIMLGTYVLSAGYYDAYYLKAQKVRTLIKQDFDVAF
                     KRCDLIATPTAPTTAFRLGEKIKDPLQMYLSDVCTISVNLAGLPAVSLPCGFDQEGMP
                     IGMQLIGKHFDEATILRVAHHYEQSTPWHKRKPKLQDRP"
     gene            1639..>2435
                     /gene="gatB"
                     /locus_tag="VFY96_17345"
     CDS             1639..>2435
                     /gene="gatB"
                     /locus_tag="VFY96_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX5608295.1"
                     /translation="MKYEPIIGLEVHAQLLTQSKIFCGCSTQFGCEPNQNTCPVCAGF
                     PGVLPVLNKNVVEFAIKAGLATHCTIAPSSIFARKNYFYPDLPKGYQISQYELPICTD
                     GYIEVEVGGSLKRVRLTRIHIEEDAGKNIHDAHGAASLVDLNRAGVPLLEIVSEPDMR
                     SPQEAGAYLRTLRQILQYLGICDGNMEEGSFRCDANVSVRPQGSEALGVKVEIKNLNS
                     FKAVEKSIEFEIERHQEKLSEGGKLIQETRLWDDHREETRSMRSKESA"
BASE COUNT          585 a          653 c          649 g          548 t
ORIGIN      
        1 aagactagtc tcaacccgct ccgtgaggat cgagtcacca atcggccgga taccgagtct
       61 ctattggcta acgcaccaga ccgagatggg acattcttca aagttcccaa aatcatcgaa
      121 tgagctttgc cgaactgacg cttcacgacg ccgcggacaa attacgcaag cgcgaattca
      181 ccgcggtgga attgacggaa gccgtctatg agcggatcgc cgaaaccgag cctcgtgtct
      241 gtgcttactt gacgctggca cacgacagcg ccttagaaga cgcggcgcgt gcggatgaaa
      301 aacgaaaaaa tactgctagc ggtgcgccgc tgttgggcat cccgcttgcg atcaaagata
      361 actttctgac caaaggtgtg cgcacgacct gcgcgtccaa aattctcggt gatttccttt
      421 cgccttatga cgccaccgtt attagcaaaa ttcgttcggc cggcgcggtg atcatcggta
      481 agaccaattt ggatgagttc gccatgggct cttcagcgga aaactcagct ttttttccga
      541 cccataatcc atggaacctt gaacgcattc ctggcggttc ctcgggtggt tctgcggcag
      601 cggtagccgc ggacgagtgc actgcggctc tggggacaga cacgggcggc tcgatccggc
      661 agccggcggc ctgttgtggc gttgtcggtc tcaagccgac ctatggtcgg gtcagccggt
      721 tcggaatcat cgccttcgcc tcttcgatgg atcaggtcgg tccaatgacc aaagacgtgc
      781 gcgacagcgc gttattgcta gaagccatcg ccggttacga cccggcagat tcgacttcgg
      841 tgaattcgac cgtgccgcgc tattcggaga tgctcgccgg tgatattaaa cgaattcgcg
      901 tcggcgttcc caaggagtat tttgcttccg gtgtagcagc tgaagtaaaa gatgctgtgc
      961 ggaaggcgat cggtgagttg gagaaacaag gcgcagttat cgaagagatc tccttgccgt
     1021 ctaccgaata cgctattgct gtttactata tcgtggccac ggcagaagcg agctcgaatt
     1081 tggcccgcta tgacggaatg cgctacggtc atcgcgccaa cgccggagat ctactggata
     1141 cctatacgct cagccgtgca gcaggattcg gcgcagaagt aaaacggcgc attatgctcg
     1201 gcacatacgt tctatcagcg ggttattacg acgcctatta tctaaaagct cagaaagtcc
     1261 gtactttgat caagcaagac ttcgacgtgg cgtttaagcg ctgcgacctg atcgctactc
     1321 ccactgcgcc gactaccgcg tttcgactgg gtgagaaaat taaagatcct ttgcaaatgt
     1381 atctttcaga tgtttgcaca atctcagtca atctggctgg cttaccggca gtttcgctcc
     1441 cctgcggctt cgaccaggaa ggcatgccga tcggcatgca gctcattggc aaacacttcg
     1501 acgaagcgac aatcctccgc gtcgcgcatc attatgaaca gtccacgcca tggcacaagc
     1561 gaaaaccaaa gctccaggat cgaccatgaa aattcaccag cagtgttttc aattttctgt
     1621 tttcggctct ctcgggctat gaaatacgag ccgatcattg gactcgaggt ccacgcccaa
     1681 ctactcacac agtccaagat cttctgcggt tgttcgaccc aattcggttg cgaaccgaat
     1741 cagaacacct gtccggtatg cgccggcttt ccgggagttt tgccggtgct caacaaaaac
     1801 gtcgtcgagt ttgcgatcaa agcaggcctg gctacccatt gcacgatcgc gccttcgagc
     1861 atttttgctc gtaaaaatta tttctatccc gatttgccca agggctatca aattagtcag
     1921 tacgaactac ctatatgcac tgatggttat atcgaggtcg aagtcggtgg atcactaaag
     1981 cgagttcgct tgacgcgcat tcacatcgaa gaagacgccg gcaagaacat tcacgatgca
     2041 cacggcgccg ccagcttggt cgacttgaat cgcgccggcg tgccattgct cgagatcgtc
     2101 agcgaacctg atatgcgaag tccacaggaa gccggagcct acctcagaac cctcagacaa
     2161 attctacaat atctcggcat ctgtgatggc aacatggagg aagggagctt tcgctgtgac
     2221 gcgaatgtct cggtccggcc gcaaggaagt gaagcgctgg gtgtcaaggt cgaaatcaag
     2281 aatctcaact cgttcaaggc tgtcgaaaaa tccatcgagt ttgagattga aaggcaccaa
     2341 gagaagcttt cagaaggcgg taagctgatt caggaaaccc gtttatggga tgatcatcgg
     2401 gaagaaacgc gttccatgcg gtcgaaggaa tcggc
//
LOCUS       DASULZ010000844         4631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_400641, whole genome shotgun sequence.
ACCESSION   DASULZ010000844 DASULZ010000000
VERSION     DASULZ010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400641"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VFY96_17350"
     CDS             <1..437
                     /locus_tag="VFY96_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HEX5608296.1"
                     /translation="PPYWINMGAVAISTLSGATLILDAQRSPLLESMLPFLRGFTLFF
                     WATGTWWIPMLVILGIWRHLYKRFRLTYDPLYWGIVFPLGMYTACTFQLAKAMKLEFL
                     FWIPRVFVYIALFTWAATFTGLLRSMGTEFKTIRALQARLGS"
     gene            complement(416..2245)
                     /locus_tag="VFY96_17355"
     CDS             complement(416..2245)
                     /locus_tag="VFY96_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX5608297.1"
                     /translation="MSDLVETLEDRLADFTRDRRVLLLSGMALIIGCLSAVVAWILVW
                     LIATITNVAFYGQLSSKLQSPEHHHLGYYMILVPIIGGLIIGVIARYGSDKIRGHGIP
                     EALEAILFGRSQMEPKVAVLKPLSSAISIGTGGPFGAEGPIIMTGGAVGSIFAQFFQL
                     SAAERKTLLVAGASGGMAAIFASPVSAALLAVELLLFEWRPRSFIPVAIAAVSAAALR
                     VPLLGAGPIFPVQPHAMLNSEGLVFAFLVGIAAGLGSGLLTTLVYAFEDLFNKLPIHW
                     MWWPAIGGLFIGIGGLIEPRVLGVGYETIHELLVGHIVGAALLGILIGKSLVWSLALG
                     SGTSGGVLAPLLMMGGALGAGLAKFIPVGDTGVWTLIGMAGMMGGTMRSPLTAMIFAL
                     ELTQDLNLLPGLLAGCVAAHGVTVLLLRRSILTEKVARRGYHVMREYGVDPLTMVRVG
                     EVMDKNPPLIPAKMSLAELSDRIARGEPEVSRHEGLLLVDDKQQLAGMITRGDVIRAL
                     AENSDNDITVSSAGSHNVIVTYADELLYEAVSKMVHNNIGRLPVVSRRDPRRIVGYIG
                     RSNLMAGRLRHFEDEYVRERRYLRRRQAQAGALAPPNQEPSRA"
     gene            complement(2410..3258)
                     /locus_tag="VFY96_17360"
     CDS             complement(2410..3258)
                     /locus_tag="VFY96_17360"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5608298.1"
                     /translation="MAVRDDTQELLLDVRVAEASRIYKIYLANEKKILGSIAVALFLI
                     AWELVGNVFQWINPMFMSAPSLIFKAGYEMFRSGEIYHDLYVSGIEFLGGYFLAAAVG
                     IPFGIMTGWYKRMSYIFDPFVNALNATPRVALLPLVIIWLGIGILSKVGIIFLGAVFS
                     ILINTRDGVKTTPANLLTAARSFGASEWMVFKTVVLPSTIPFILTGLRLAVGRALVGV
                     LVGELYAATAGIGFMITVAGATFQTDKVFVGVGIFALSGMIGMELLTKVERRFDKWRP
                     QVGSEQ"
     gene            complement(3442..>4631)
                     /locus_tag="VFY96_17365"
     CDS             complement(3442..>4631)
                     /locus_tag="VFY96_17365"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5608299.1"
                     /translation="QSHRRKLRYADLVSTGPGTLAGRFMRRFWHPVYRAEELLAAQAK
                     PIEIMGERFTLYRGEDGNPHVTEFRCPHRGTQLSAGWVEGDSIRCMYHGWLFNSAGQC
                     IEQPAEAKSFAAKIRIKSFPTREYLGLIFTYLGEREAPPFPRFSHMEEDGVLEVLSTE
                     VWPCNFFQRIDNNGDTYHVPFVHRGAYSASTVNNRSGLPEISKVESPWGTTGYASFKE
                     GWRNVFQFFMPNVYAFRNPSPEPECAWEDRMQWDVPLDDAHSLEFRLRRLPLRGSAAD
                     QYRARRDITAAQPKLSVAQMGDAVLSGKISFQDLDHFFKDKISLVHTQDYVAQVGQGS
                     RADRTREHLGLSDTGVILFRKIWERELRAFADRSPLKSWVLPQDAEITFQEAPERLLQ
                     KAR"
BASE COUNT         1114 a         1324 c         1278 g          915 t
ORIGIN      
        1 cgccacccta ttggatcaat atgggcgcag tagcgatttc gacactatcc ggcgcgacgt
       61 tgattctcga cgcacagcga tcgccgctgc tggaaagtat gctgcctttc ctgcgagggt
      121 tcacgctatt cttctgggcc acgggcacct ggtggattcc gatgctggtg atattgggga
      181 tttggcgtca cctatataag cgcttcaggc tcacttacga tccactctat tggggcattg
      241 tgttcccgct gggcatgtat accgcctgca cgtttcagct ggccaaggcg atgaagttgg
      301 aatttttgtt ttggattccg cgggtatttg tctatattgc gctgtttaca tgggccgcaa
      361 cattcactgg cttgctgcgc tcgatgggga cggaatttaa aacaattcgc gcgcttcaag
      421 cgcggctagg ttcctgattc ggtggagcga gggcgcccgc ttgcgcttgt cgtcgccgta
      481 agtatctccg ctcgcgaacg tattcgtctt cgaaatgacg cagccgaccg gccattagat
      541 tactcctgcc aatgtagccg acaatgcggc gcggatctcg ccggcttact acaggtaagc
      601 ggccaatatt gttgtgcacc atcttactga ccgcttcgta taaaagctcg tcggcgtagg
      661 tgacaatcac attgtgactg cccgcgctag aaacggttat gtcattgtca ctattttccg
      721 ccagagcacg aatgacatcg ccgcgggtaa tcatgccggc gagctgctgt ttgtcgtcga
      781 ccagcaacag cccttcatgc cggctcactt cgggctcacc acgcgcgatc cggtcggaca
      841 attccgcgag agacattttg gccggaatga gcggcgggtt tttgtccatc acttcgccga
      901 cgcgcaccat ggtgagcgga tcgacaccgt actcacgcat gacgtgatag ccacgacggg
      961 cgactttttc ggtgagaatc gatcggcgca atagtaatac ggtcacgcca tgggctgcga
     1021 cgcagcccgc gagcaagccc ggcaagagat tgagatcctg cgtcagttcc aatgcgaaaa
     1081 tcatcgccgt gagcggcgag cgcatcgtgc cgcccatcat gcctgccatg ccgatcaacg
     1141 tccatactcc ggtatcgccc acgggaataa acttggccaa accggctccc aacgcgccgc
     1201 ccatcatcaa caatggcgca agcacgccgc cagacgttcc ggagccgagc gccagcgacc
     1261 acaccaaaga cttgccgatc agaatgccca gcagagccgc gccgacaata tgaccaacca
     1321 acaattcatg gatggtttcg taccccacgc cgagcacgcg cggttcgatc aatccaccga
     1381 tgccgatgaa taaccccccg atcgcaggcc accacatcca gtgaatcggt aacttgttaa
     1441 ataaatcttc gaaggcataa accagagtgg tgagaagtcc ggagccgagt ccggccgcga
     1501 taccaaccaa aaaagcaaac accagacctt ccgaattcaa catggcatgg ggttgaactg
     1561 gaaaaatcgg accggcgccg aggaggggaa cgcgaagagc ggcagctgaa acggcggcga
     1621 tcgcaaccgg aataaatgag cgcgggcgcc actcgaacag caggagttcg accgcgagca
     1681 gcgccgcgga aacgggcgag gcaaagattg ccgccatgcc gcccgaagcg ccggcgacga
     1741 gcagtgtttt tcgttcggcg gctgagagct gaaaaaattg cgcgaagatc gaacccacgg
     1801 cgccgcccgt cataatgatc ggcccctcag cgccgaacgg gccaccggta ccgatcgaaa
     1861 tggcagaaga aagcggctta agcacggcga ccttgggctc catttgactg cgaccgaaaa
     1921 gaatcgcttc aagcgcctcg ggaatcccgt gaccgcgaat cttgtctgag ccgtaacgcg
     1981 ctatcacgcc aatgatcagg ccgccaataa tgggcacgag aatcatgtaa tagccgagat
     2041 ggtgatgctc gggagattga agcttcgagg aaagctgccc gtaaaaagca acattggtga
     2101 tcgttgcgat caaccagacg aggatccagg cgacgacagc gctgaggcag ccgatgatta
     2161 aggccattcc cgaaagcagg aggacccgtc tgtctcgagt aaaatccgca agccggtctt
     2221 ccagggtttc gaccaaatca ctcatgagca tcacacctct gcatgattca atgttgtcaa
     2281 gcgaggagcg caggaaggca aagatattcg gcgcgatcct cgaaggcgga tcaatcggta
     2341 ccgaagcttt cacaagctgc ggcgaacgct atcgccgcta tctaaaaagg ggtctggcga
     2401 gagaacgatc tattgttcgg atcccacctg cggccgccac ttgtcaaacc gtcgttccac
     2461 cttggtcaac aactccatgc caatcatgcc gctcaaggcg aaaatgccga cgccgacaaa
     2521 aactttgtcg gtctgaaatg tcgcaccggc aacggtgatc atgaagccaa ttccggctgt
     2581 cgccgcgtaa agctcgccca ccaacacgcc cacgagagcg cggccgactg caaggcggag
     2641 cccggtaaga ataaaaggaa tcgtcgatgg aaggacgacc gttttaaaga ccatccattc
     2701 ggaggcaccg aagctccggg cggcagtcaa gagattcgcc ggcgtggttt ttacgccatc
     2761 gcgagtgttg ataagaatcg agaacaccgc gccaagaaaa ataatgccga ccttcgacag
     2821 gatgccgata ccgagccaga taatgactag cggaagcaga gcgacacgcg gcgtggcatt
     2881 gagcgcattg acgaaaggat cgaagatata gctcatccgt ttgtaccacc cggtcatgat
     2941 gccgaatgga attcctacgg cagcagctag aaaataaccg ccgagaaatt cgatgccgct
     3001 aacgtagaga tcatgataga tctctccgga gcgaaacatc tcgtagccgg ctttaaatat
     3061 cagcgacggt gcgctcatga acatcgggtt gatccattga aagacgttgc cgacaagctc
     3121 ccaggcgatc aggaacagag cgacggcgat gctgccaaga attttctttt cattagccaa
     3181 atagatctta tagatccggg aagcctccgc aacccgaacg tcgaggagca actcttgtgt
     3241 atcgtcacga actgccattt ttgtatccct ccatcgccaa ttgagcgccc gacggtcaca
     3301 atgtatcgca atacgatatt atcggtccaa agctctcctg tcaaccggcc gatatcgtca
     3361 cgctgagcgg cggattgaat aacccagagg cgaccatcac gggcgctgct cgggagcacg
     3421 tgttcacttc gggatccatg gtcaccgtgc tttctgaaga agcctttccg gtgcctcctg
     3481 gaacgtgatt tccgcgtctt gcggcaaaac ccagctcttc agcgggcttc ggtcggcgaa
     3541 tgcgcgcagc tcgcgttccc aaattttccg aaacaggatc acgcccgtgt ccgaaagacc
     3601 gaggtgctcg cgtgttcggt ctgcgcggga tccctgaccc acctgcgcaa cgtaatcttg
     3661 cgtgtgcacg agagagattt tgtctttaaa aaaatgatcc aagtcctgaa agctgatttt
     3721 gcccgacaga accgcgtcac ccatttgggc caccgacagc ttcggctgtg cggccgtgat
     3781 atcacggcgg gcgcgatact gatcggcggc ggaacccctc agtggtaaac ggcgcaagcg
     3841 aaattccaat gaatgcgcgt cgtcgagggg cacatcccac tgcatgcgat cttcccaggc
     3901 gcactccggc tccggcgagg ggttacgaaa cgcgtagacg ttgggcatga aaaactggaa
     3961 aacattccgc caaccttctt taaagctcgc gtagccggtg gtgccccagg ggctttccac
     4021 ctttgagatc tccggtagac cgctgcgatt attgaccgtc gaggccgaat aggcaccgcg
     4081 gtgaacgaaa ggcacatgat aggtatcacc gttgttatcg atgcgctgaa aaaaattgca
     4141 cggccacact tcggttgaca gaacctcgag aacgccgtcc tcttccatgt gtgaaaagcg
     4201 cgggaacggc ggcgcttcgc gctcgccaag gtatgtaaaa atcaaaccga gatactcgcg
     4261 tgtaggaaaa cttttgatgc gaatctttgc cgcaaaggac tttgcttccg ccggttgttc
     4321 gatgcattga ccagcgctgt taaacaacca gccgtgatac atgcagcgga tcgaatcgcc
     4381 ttcgacccac ccagctgata actgcgtacc ccgatgcggg caacgaaatt cggtgacgtg
     4441 cgggttacca tcttcgccgc ggtaaagcgt aaagcgctcg cccatgatct cgatcggctt
     4501 ggcctgagcg gcgagaagct cctcggcgcg gtaaaccgga tgccaaaacc tgcgcatgaa
     4561 tcttcccgcg agagtgcctg gccccgtgga gacaaggtca gcgtaacgca gcttccgacg
     4621 atggctttgg g
//
LOCUS       DASULZ010000845         5215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1315591, whole genome shotgun sequence.
ACCESSION   DASULZ010000845 DASULZ010000000
VERSION     DASULZ010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315591"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VFY96_17370"
     CDS             <1..392
                     /locus_tag="VFY96_17370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608300.1"
                     /translation="ILRSKGYTLMWPPEADIRPYQSGHAEQVVKVDWREGSVFSPPSG
                     WFHQHFNTGNEKARQLAFRYSGQSGKYLLGAWRAINKEGVRTSVREGGTMIEYEDEDP
                     QIRADFEMAIKRIGVPMEMPEFTYAEL"
     gene            397..1515
                     /locus_tag="VFY96_17375"
     CDS             397..1515
                     /locus_tag="VFY96_17375"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5608301.1"
                     /translation="MKMAFRSVALFLDLLIMGSIVHAQTGKPGWQTDWEKTIELAKKE
                     GKVVVSIPASTELRAEIEKNFEKRYGIDVEPVVGRAANVVRKIVDEAKAGVRYVDLHM
                     GGSESIVTGLLPEGVLDSVEPSMILPEVKDPKQWWGGHVWVDNAKRFIYSTLAYQTES
                     LWFNSQLMKAADIRSFDDLLDERWKGKIGYLDPRTPGSGASMWSYLREIKGEEYLKKF
                     AAQKPLLSRDQRVLAEILAKGKVALVIGLTYYSYAPFVKAGLPVQSLPTPQEGVYVSG
                     GSGHLIVLKNSAHPNATKLFINWFLSREGQEVFTRSMHQASRRLDVDGKWLREFGVIP
                     AKDSLTLEQYYKLENQSEAKINRLREPAAALARKLFGS"
     gene            1559..2641
                     /locus_tag="VFY96_17380"
     CDS             1559..2641
                     /locus_tag="VFY96_17380"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5608302.1"
                     /translation="MIVFQSARVHAQPELNKGSSDWEKTLEAAKKEGRVVAAIPTSAE
                     LRKELESRFQAKFPGIELELSVSRGASNTNKIVEEQNAGVRGTDLHIGGTTSIVTALL
                     ARNLLEPVLPAMVLPEVREPKNWWGEHIWADNAKRFIYSFTAYVTETLWYNTTLAKAD
                     EITSYDQLLDPKWKGKIAILDPRTPGSGESTWGFLWKVKGEEFLKKLAAQEMMVGRNQ
                     RQLAEALARGKSAISIGVSYYTLLPFIKSGLPVKPVPYLKEGFYAGTGSGNLALLKNS
                     AHPNATKVFINWFLSKEGQSAFLRALGQPTRRLDVDTQASKEFGYTAAKEGLTPEKYY
                     AIENSSEDAVIKVRVPAMRLAEKLFQ"
     gene            2670..3770
                     /locus_tag="VFY96_17385"
     CDS             2670..3770
                     /locus_tag="VFY96_17385"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5608303.1"
                     /translation="MLKQSLGVWAVLMMSVASSLAQSKPSEWERIVEAAKKEGKVVVS
                     IPPSPELRKTMELAFTRRYGIATEFVPARGGAIIQRMVSEAKSGAQYFDLHIGGTESV
                     VTGLLPENILEPVEPFFLQPDVKDPKQWWGGHIYIDNARRFVYNFVAYQTVSLWSNPN
                     EYKPAEFKSFDDLLKQKLYGRIGISDPRTPGSGSSMWSYMHYIKGEDYLRRLVAQKLF
                     ITRDLRLLAENLAKGKVAVTSGIGYSEYYPFIKANLPVVALPVPKEGLYVSGGYGHLV
                     VFKNQPHPNATKVFINWLLSHDGQEVFARSMGVGTRRLDVETKWLKEYGVISAKDFLT
                     LDQFYKLENQSEEKINKIREPAAVVARKLLGS"
     gene            3840..>5215
                     /locus_tag="VFY96_17390"
     CDS             3840..>5215
                     /locus_tag="VFY96_17390"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX5608304.1"
                     /translation="MAFADLREWIDDLEERKDLKRIGVEVGWDEEIGAITREVSSQFG
                     PALLFENIRDHKSSFCRRLFTNGTGTRERVCRFIGVPESTSYRDMVPIFKERFSHPVK
                     PIRVARGPVKENILRGPSIDLTQFPVPKWNPLDGGRYIMTSATVVTRDPDSGILNGGT
                     YRGMITGRNTIGVLLAMTQGWGKHFSKYKNRGQEMPVAVVIGWDQSLFVAASTPVNLP
                     EYEMAGSLRGAPVELVKCELSELLVPASAEIVLEGFVSADPTTYTVEGPFSEYPGYYG
                     GSKSPKHTIRVECITHRNDPIFHGCLTGASPGRTNEGTTWTPATFSAMAWHYLEQAGV
                     PNVTAVWRGKWPETMRVQIRKSHRGHAQQVATALWGCHLGNYAAKHVIVVDDDIDIHD
                     WEAIEWALCYRVNAGLGDISFFAGTSGSMLDPSVPLEERNSVKYGHGTWTRVLIDATI
                     NWRLEPQE"
BASE COUNT         1242 a         1330 c         1542 g         1101 t
ORIGIN      
        1 tgatcctacg ctcaaagggc tatacgctta tgtggccgcc cgaggcggac attcgccctt
       61 atcaatcagg tcacgccgag caagtggtca aagtcgactg gcgcgaaggc agcgtgttca
      121 gcccgccgag cggctggttt catcagcatt tcaataccgg caacgaaaaa gcgcgccagc
      181 tcgcgtttcg ttatagcggt caatcgggaa agtatctgct cggcgcttgg cgcgcgatca
      241 ataaagaggg ggttcgcacc agtgtacgag agggcggcac gatgatcgag tatgaagacg
      301 aagacccgca gattcgcgcc gattttgaga tggctatcaa acgaattggc gtgccgatgg
      361 aaatgcctga gtttacctac gccgaattgt aatttcatga aaatggcttt ccgcagtgtg
      421 gcgttgttct tagatctgct aatcatgggc agcatagtgc atgcgcagac tggcaaaccg
      481 ggatggcaga ccgattggga aaaaaccatc gagctcgcca agaaagaggg caaggtcgtg
      541 gtttccattc cggcgagcac agaactccgc gccgaaatcg agaaaaattt cgaaaaacgc
      601 tatggcatcg acgtcgagcc tgtggtgggg cgcgctgcca acgtcgtgcg caaaatcgtc
      661 gatgaagcga aggccggcgt gcgctacgtc gatttgcaca tgggagggag cgagtcgatc
      721 gtcaccgggc tcttgccgga aggtgtcctg gattcagtcg agccgtcgat gatccttcct
      781 gaagtgaagg atccgaagca atggtggggc ggccatgtct gggtggacaa tgcgaaaaga
      841 tttatttatt cgacgttggc ctatcagacc gagagcttgt ggttcaattc gcagttgatg
      901 aaagccgcag acatacgttc gttcgacgat ctcctggacg aacgctggaa aggaaaaatc
      961 ggttatctcg atccgcgcac tccgggctcc ggcgcatcga tgtggtctta tctacgtgaa
     1021 ataaagggcg aggagtatct caaaaaattt gcggcgcaaa agccgttatt gagccgggac
     1081 cagcgcgtgc tcgcggaaat cctcgcgaaa ggaaaagtcg cgctcgtgat tggcttgacc
     1141 tattactctt atgcgccttt cgtcaaggcg ggcttgccgg tgcagtcttt gccgacaccc
     1201 caagaaggcg tctatgtcag cggcggaagc gggcacctga tcgttttgaa aaattcggcg
     1261 cacccgaatg ctactaaact cttcatcaac tggtttctta gccgagaagg gcaggaagtt
     1321 tttacgcggt caatgcatca ggcgtcacgc cgattggatg tcgatggcaa gtggctgcgg
     1381 gagttcggcg tcatacctgc aaaggacagt ttgaccctcg agcaatacta caaactggaa
     1441 aatcaatcgg aagcaaagat caaccgtctg cgcgaaccgg cagcggcgtt ggcgcgaaaa
     1501 ctgttcggct cataggcttc tctgtatgtc gcgctcggca atattcctga gcttattagt
     1561 gatcgtattc caaagcgctc gcgtccatgc acagccggag ctaaataagg ggagcagcga
     1621 ttgggaaaaa actctcgagg cggcaaaaaa agaaggtcgg gtggttgcgg cgattcctac
     1681 cagtgctgaa ctgcgcaaag aactcgagag tcgttttcaa gcgaaatttc ccggcatcga
     1741 gctcgagctc agtgtttcac gcggcgcgag caacaccaac aaaattgtcg aagagcaaaa
     1801 cgccggtgtg cgcggcaccg atttgcacat tggcggcaca acttcgattg tcaccgcact
     1861 gctcgcgcgc aacctgctcg aaccggtatt gcccgcaatg gtgctgcccg aagtgcgcga
     1921 gccgaagaat tggtggggtg agcatatatg ggcggataac gcgaagcgat ttatctacag
     1981 tttcaccgcc tatgtcaccg aaaccctttg gtataatacg actctggcca aggccgacga
     2041 aatcacctcc tacgatcaac tgctcgatcc taaatggaaa ggtaagatcg ccattctcga
     2101 cccgcgcaca ccgggttcgg gtgaatcgac ctggggcttt ctgtggaagg tgaaaggcga
     2161 ggaattctta aaaaaactcg cggcgcaaga aatgatggtc gggcgcaatc agcgccaatt
     2221 agcggaagcg ctggcgcgcg gcaagtcggc gatctccatc ggcgtatcct attatactct
     2281 tctccccttc atcaaatcgg gcctgccggt aaaaccggtg ccgtacctca aagaaggctt
     2341 ttatgccggc acgggcagcg ggaatctcgc tctgctgaag aattcagcgc atccgaacgc
     2401 gaccaaagtt tttataaatt ggtttctgtc caaagaggga cagagcgcgt ttttgcgggc
     2461 attgggacaa ccgacccggc gtttggatgt cgacactcaa gcgagcaaag agttcggcta
     2521 cacagcggca aaagaagggc tcacgccgga gaagtactac gcgatcgaga acagctccga
     2581 ggacgcagtg ataaaagtgc gcgtgccagc gatgcggctc gccgagaaac tctttcagtg
     2641 accaagtcat ggaacaaagc ataggagcta tgctgaagca gagcttaggc gtatgggcgg
     2701 tgctgatgat gagcgtggca tcgagcttgg cgcaatccaa accgagcgaa tgggagcgga
     2761 tcgtcgaggc ggcaaagaaa gagggaaagg tggtcgtgtc aattccgccg agccccgagt
     2821 tacgcaaaac catggaactc gcatttacgc gcaggtatgg gattgccact gagtttgtgc
     2881 cggcgcgcgg cggcgccatt attcagcgca tggtcagcga agcgaaaagc ggcgcgcaat
     2941 attttgattt gcacattggc ggcaccgagt cggtggttac gggactgttg ccggaaaaca
     3001 tcctcgagcc ggttgagccc tttttccttc agcccgacgt taaagatccc aagcaatggt
     3061 ggggtggcca tatctatatc gataacgcta ggcgctttgt ctacaacttt gtcgcttacc
     3121 agacggttag tctttggagc aacccgaacg aatacaagcc ggcggagttc aagtccttcg
     3181 acgatttgtt gaagcaaaaa ctttacggca ggatcggcat cagcgacccg cgtacgccgg
     3241 gctctggatc atcgatgtgg tcttatatgc actatatcaa aggcgaggat tatttgaggc
     3301 ggcttgtggc ccaaaagctt ttcatcacgc gagaccttcg cctgttggcc gaaaatctcg
     3361 cgaagggtaa agtcgccgtc acctccggca tcggctactc cgaatattat ccgtttatca
     3421 aagccaatct tccggtggtg gccctacctg tgcccaaaga aggactctat gtaagcggcg
     3481 gctatggcca tctcgtcgtc ttcaagaatc agccgcaccc caacgcgacc aaagttttta
     3541 tcaactggct cttaagccat gacggtcaag aggttttcgc tcgtagcatg ggtgttggca
     3601 cccgacgcct ggatgtcgag accaagtggc tgaaagaata cggcgtgatc tcggccaagg
     3661 attttttgac tctcgatcag ttctataagc tggaaaatca gtccgaagaa aagatcaaca
     3721 aaatcagaga gccggcggcg gtggtggcgc gtaaattgtt gggatcgtag cggggacgac
     3781 gcctgggtac ccagcggaac gcgtcgagac atcgacaatt cgcatgagga aagaaaattg
     3841 tggcgtttgc tgatttgcgc gagtggattg acgatctcga ggaacgaaag gatttgaagc
     3901 ggatcggcgt cgaagtcggt tgggatgaag agatcggcgc gatcacccga gaagtctcca
     3961 gtcagttcgg accggccttg ctgttcgaga acatcagaga tcacaagagc agcttttgtc
     4021 ggcgtctctt caccaacggc acgggcacac gagagcgagt ttgccggttc atcggagttc
     4081 ccgaatcaac atcctatcgg gatatggtgc cgatctttaa agagcggttt tcccatcctg
     4141 tgaagccgat ccgcgttgcg cgcgggcccg tgaaggaaaa catcttgcgt ggaccgtcca
     4201 tcgatcttac tcagttcccg gtgccgaaat ggaatccgct cgacggcggg cgctacatca
     4261 tgacttcggc gaccgtggtg acgcgggatc cagatagcgg cattctcaac ggcggcacct
     4321 atcgcggcat gatcaccggg agaaacacca tcggtgtttt gctggcgatg acccaagggt
     4381 ggggcaagca ctttagcaag tacaaaaatc gcggacagga gatgccggtc gcggtggtga
     4441 tcggctggga tcagagcttg tttgtcgccg cgtcgactcc ggtcaatctt cctgagtatg
     4501 agatggcggg atcgctccgg ggcgctccgg tggaactggt gaaatgcgaa ttaagtgagc
     4561 ttttggttcc agccagcgcc gaaatcgtgc tcgagggctt tgtctcggcc gacccgacga
     4621 cttacaccgt cgaaggaccg ttcagtgaat acccagggta ctacggcgga agcaaatcgc
     4681 cgaaacatac cattcgtgtc gagtgcatca cgcatcgcaa tgatccgatt tttcacggtt
     4741 gtttgacggg tgcgagtccg ggaagaacca acgaaggcac aacctggacg ccggcgacct
     4801 tctcggcgat ggcgtggcac tatctcgagc aagccggggt gcccaatgtc acggctgtct
     4861 ggcgcggcaa atggccggaa acgatgcgcg tgcagatccg caaatcgcac cggggtcatg
     4921 cgcaacaggt cgccacagct ctctggggct gccacctcgg gaattatgcg gccaagcatg
     4981 tgatcgttgt ggacgatgat atcgatattc acgattggga agcgatcgag tgggcgcttt
     5041 gttatcgggt caacgccggt cttggcgaca tttccttttt cgccggtacc agtggctcga
     5101 tgctcgatcc cagcgtgccg ctcgaggagc gcaattcggt taaatacggc cacggcactt
     5161 ggacgcgtgt attgattgac gcaaccatca actggcggct cgagcctcaa gagca
//
LOCUS       DASULZ010000846         2859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1315649, whole genome shotgun sequence.
ACCESSION   DASULZ010000846 DASULZ010000000
VERSION     DASULZ010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315649"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2092)
                     /gene="glnD"
                     /locus_tag="VFY96_17395"
     CDS             complement(<1..2092)
                     /gene="glnD"
                     /locus_tag="VFY96_17395"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEX5608305.1"
                     /translation="MDAVAISLALLEDVHAETDLKRLARRYIEAGRRELFERHRDGAG
                     GLDVVSAWSTVMDHLIRHLFMLISSECARRFPTPNPRFALLAQGGYGRGELNPLSDID
                     LLFLYSWKVSPFIEATTEKLLYTLWDAGLEVGHATRSIAECMRLAEIDMKVRTSLLDT
                     RFLCGDYALYQDFEKRIESRLVKKGVKRFIREKLEENRARHEQFGGSVYLLEPDVKEG
                     EGGLRDIQTARWIARAKLKIKDLDGLAIAGMVDARDIAQLKESQDFLLRVRNELHFST
                     GKHQDQLTFEHQEKVSAALRFEGEGTLRAVEVFMRCYYLHAAHINRLSSLIIHRVTDS
                     DKPGFSASYMFGRTLREGIRLSKNHITVIKPEIIRKQPENLIRIFDDAQKYNCALTHE
                     TRELLRQNLDLIDDEFRRSAEANLPFFSILKWKDSVYETVLEMHRAGVLGAFIPEFGR
                     LLCMVLHDAYHIYTVDQHSLRLIKEIERLKAGEYAESSPLLTQLAREVEKIELLYLGL
                     MFHDIGKGFGGGHSEIGAHMVHAIARRMRLNADDGALVEFLVRHHLLMTHIAFRRDLE
                     DEKTILDFARTMGTVTNLKLIYLLTFADVKAVGPEVWNPWKASLLGELYVKALNVLEE
                     AEKGEFQRQDVRAVIRRVQTRIKRQLSKDYGEEEIDRFLEIMPDRYFSSTPEDDIVEH
                     FEIMQRFDGEQAEVA"
     gene            complement(2103..>2859)
                     /locus_tag="VFY96_17400"
     CDS             complement(2103..>2859)
                     /locus_tag="VFY96_17400"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX5608306.1"
                     /translation="NKEASARRPQPTNKIGASATVSNGLRKVVLDPGHGGKDPGALAP
                     GGIAEKEIVLSIAKKLAVKLKNEMGTDVVLTRKDDRFVTLEDRTALANREDADLFISL
                     HVNASPNIDAKGIETYYLDNTTDEAAIRLAARENATSRKNISDLQFILSDMMQNMKLE
                     DSITLAHRLQTSLVSGMSKFMGDVKDLGVKKALFHVLVGARMPSVLVEMFFITNKTES
                     RVMADASYQDAMVNALFEGIQKYAQSNLIAKTL"
BASE COUNT          618 a          811 c          745 g          685 t
ORIGIN      
        1 tcgccacctc cgcctgctcg ccgtcgaatc gttgcatgat ctcgaaatgc tcaacaatgt
       61 cgtcttccgg cgtagatgaa aaatagcgat ccggcatgat ctcgaggaat ctgtcgatct
      121 cctcctcgcc ataatctttc gagagctggc gcttgattcg ggtttgcacg cgacgaatca
      181 ccgctcgaac gtcctggcgc tggaattctc ccttttccgc ttcttcaagg acattgagag
      241 ctttgacgta gagttcaccg agcagcgacg ctttccaggg attccatact tccgggccga
      301 cggctttgac atcagcgaag gtcaacaagt agatcagttt cagattcgtg accgttccca
      361 tggtccgggc gaaatcaaga atcgtttttt cgtcctccaa atcgcggcga aacgcgatgt
      421 gcgtcatgag cagatgatgc cggacgagaa attcgaccag cgcaccgtcg tcggcattca
      481 ggcgcatgcg ccgcgcgatc gcatgaacca tatgcgcgcc gatttcggaa tggccgccgc
      541 caaagccctt accgatgtcg tgaaacatca ggccgagata caaaagctcg attttttcca
      601 cttcgcgcgc aagctgcgtc agcagcggcg agctttcggc gtattcgccg gccttcagcc
      661 gttcgatttc cttgatcaag cgcaaagaat gctgatcgac ggtgtaaatg tgataggcat
      721 cgtgcagcac catacagagc agcctgccaa attccggaat gaacgcgccg agaactccag
      781 cccgatgcat ctcgagcacc gtctcgtaga cagaatcctt ccatttgaga atgctgaaaa
      841 acggcagatt ggcctcagcc gagcggcgga attcatcatc gatcagatcg agattctggc
      901 gcagcagctc gcgcgtttca tgcgtcagag cgcaattata cttctgtgca tcgtcaaaaa
      961 tgcggatcaa gttctcgggc tgcttcctga tgatctccgg tttgatcaca gtgatatgat
     1021 ttttcgatag gcgaatacct tcacgaagcg ttcggccgaa catataactt gcgctgaagc
     1081 cgggcttatc ggaatcggtg acccgatgga taatcaggga tgacagccga ttgatatgag
     1141 ccgcgtgcag ataataacag cgcatgaata cttcgacagc gcgcagggtt ccctcacctt
     1201 caaaccgcag cgccgcactg actttttcct ggtgctcgaa agtcaattgg tcctggtgct
     1261 tgccggttga aaaatgcaat tcattgcgca cccggagcaa aaaatcctga gactctttga
     1321 gttgcgcgat atcacgcgcg tcgaccatgc ccgcgatcgc cagcccatcg agatctttta
     1381 ttttgagctt agcacgcgca atccaacggg ccgtttgaat atcccgcaga ccgccctcac
     1441 cttccttcac gtcgggctcg agcaaataaa ccgagccgcc gaattgttca tgccgagctc
     1501 ggttctcttc gagcttttcg cggataaatc gtttgacccc cttttttacc agccgcgact
     1561 cgattctttt ttcgaaatcc tgatagagcg cgtaatcgcc gcatagaaat cgggtgtcaa
     1621 gaagcgacgt gcgcactttc atgtcgatct ccgcaaggcg catgcattcg gcgatgcttc
     1681 gcgtcgcgtg cccgacttcc agtccggcgt cccaaagggt atagagcagc ttttccgtgg
     1741 tcgcctcgat aaacggcgaa accttccagg aatagagaaa caagagatcg atatcggaga
     1801 gtggattcag ctcgcctcga ccgtaaccgc cttgagccag gagcgcgaag cgcggattgg
     1861 gcgtggggaa ccggcgggcg cactcggagc ttatcagcat gaaaaggtgc cggatcaggt
     1921 gatccatgac ggtggaccat gcggagacca cgtccaggcc gcccgcaccg tctcgatggc
     1981 gctcgaacag ttcgcgccgg cccgcctcga tatagcgccg cgccaaccgc tttaaatcgg
     2041 tttcagcgtg cacgtcctcg agcaatgcca atgaaattgc tacggcgtcc acggtttctg
     2101 ccctacagcg ttttggcgat caaattcgat tgcgcgtact tttgaatccc ctcgaacaaa
     2161 gcattgacca tcgcgtcttg gtagcttgca tcagccatga cgcggctttc ggttttatta
     2221 gtgatgaaaa acatttccac gagaacgctg ggcatgcgcg ctccgacgag tacgtgaaat
     2281 agggccttct tcacgccgag gtccttcacg tctcccatga atttgctcat cccgctcaca
     2341 agcgacgtct gtagccggtg ggcgagcgta atcgaatcct cgagcttcat gttttgcatc
     2401 atgtcgctca ggatgaattg caaatcggag atgttttttc gcgacgtggc gttttctctt
     2461 gccgcaagcc ggatcgccgc ttcgtcagtg gtgttgtcca ggtaatacgt ttctattcct
     2521 ttggcatcga tattcgggct ggcattgaca tgcagtgaaa tgaacagatc ggcgtcttcg
     2581 cgatttgcca gcgctgtgcg atcttccaac gtgacgaagc gatcatcctt gcgcgtgagc
     2641 accacgtccg tgcccatttc attcttgagc ttcactgcga gtttctttgc gatgctgagc
     2701 acaatctctt tttcggcaat accgccgggc gctaaggcgc ccggatcttt tccgccgtgt
     2761 cccggatcca agacgacttt acgcagccca ttggaaacag tcgctgatgc accgatcttg
     2821 tttgtcggct gtggcctgcg cgcagaagct tccttgttt
//
LOCUS       DASULZ010000847         2676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1418041, whole genome shotgun sequence.
ACCESSION   DASULZ010000847 DASULZ010000000
VERSION     DASULZ010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1418041"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VFY96_17405"
     CDS             complement(<1..669)
                     /locus_tag="VFY96_17405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5608307.1"
                     /translation="MRFGTFSYNQARPWVAERQAFDELLEQILLTEQLGFDEAWFAEH
                     HHSDYGMLASPNLIIANLAPRTKRLRMGNLVSVLPLYDPMRFAEECGMLDILTNGRLN
                     VGLGRGVPKDDMKHRLDRDTAQARFEEGVEILMRAWTGETFSYSGKAWGYEEISCRPL
                     PLQKPHPPIYYGATSPDSPAMVARRGWNLALSRQPLSNCARAIQAYRAERAKHPDLAG
                     SGNAI"
     gene            complement(841..1317)
                     /locus_tag="VFY96_17410"
     CDS             complement(841..1317)
                     /locus_tag="VFY96_17410"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX5608308.1"
                     /translation="MHKGSLFFCIFLIAIAAYATFAATGWSFKTKLFPLAVSIPLLTL
                     LVIQLLLMIGGKERTGESAAMDIDFSIEVPPEVARRRVFGIFGWIVGFIALVYLLGFP
                     LTVPLFIFIYLKFQSNIGWLSTIAITVVTWGCFYLLFQSLVHIQFEAGAIPTWLGM"
     gene            complement(1310..>2676)
                     /locus_tag="VFY96_17415"
     CDS             complement(1310..>2676)
                     /locus_tag="VFY96_17415"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX5608309.1"
                     /translation="IFKMSPAEAFAFLLGMHSVAATTGDITSVLFGVPGEAISAATVV
                     DGYPMAKKGEAGRALGAALMSSLLGALIGAVALALAVPIVRPLVLTFGSPELFMLSLI
                     GISCITSLSGTSTRGQIKGFAMGLFGLLISTIGQERQSGSLRFDLGMMYLWDGLDFVP
                     VLVGVFAIPEIIDLAVRGTSIAGDRPPENLSTGVMEGVKDTFRHFWLVFRCSIIGVFV
                     GIMPGAGGGVAQWMAYAHAVQSATDSKERGGFGKGDIRGVLGPGAANNSKEGGELIPT
                     VAFGVPGSGAMAILLSGFLIMGIMPGPEMLTKHLSLTFSMVWTLAIANVITVILSLAI
                     LDQLAKLTYVRGGLIIPFILLLVFVGSYTANGQIADLIVTLIFGVLSYFMVLFGWPRP
                     PFVLGFVLGKVIETYLFISMTRYGFGWLTHPIVVALMILMVVVIAYPYIQQRRQRLRE
                     PAHA"
BASE COUNT          698 a          754 c          729 g          495 t
ORIGIN      
        1 gatcgcattg ccgctgcccg ccagatcggg gtgcttggcg cgttctgctc gatacgcctg
       61 aattgcgcgc gcgcaattgc tgagcggctg gcgggacagc gcaagattcc aaccgcgacg
      121 cgcgaccatg gccggactgt ccggagaagt cgcgccgtag tagattggcg gatgaggctt
      181 ttgtagcggt aacggccggc aggagatttc ttcgtacccc cacgctttac ccgagtaact
      241 gaacgtctcg ccggtccagg cacgcatcaa gatttcgacg ccttcctcga aacgagcctg
      301 agcggtatcg cggtcgagtc ggtgcttcat gtcgtccttc ggtacgccgc gaccaaggcc
      361 aacgtttaaa cgcccgttcg tcaaaatgtc tagcatgccg cattcttcgg caaagcgcat
      421 gggatcgtag agaggcaaaa cgctgacgag attgcccatc cgcaaacgtt tcgtgcgcgg
      481 cgcgagattg gcaatgatca aattggggga agcgagcata ccgtagtcgg agtgatggtg
      541 ttcggcaaac caagcttcgt cgaaccccag ctgctctgtg agcaagatct gctcgagcaa
      601 ttcgtcgaag gcttgtcgtt ccgccaccca tggccgtgct tggttgtagc taaaagttcc
      661 gaagcgcatg aatcattttc ctcccgcccg ttacgacaat ctcgactata ccatggttca
      721 tgttctgact tctttggggc agattcaatg tacgctgtag gatggtgcag tacgacgtca
      781 attcgatcct acaatcgcct acagaaaaag aacaggctac ctcccgtgtg tcctgacgat
      841 ctacatgccg agccaggtcg gaatggcgcc ggcttcaaac tggatgtgaa cgagagattg
      901 aaatagcagg tagaaacagc cccaagtcac gaccgtgatt gcgatcgtgg aaagccagcc
      961 gatgttactt tgaaatttta aataaataaa aataaacagc ggcacagtta acggaaaccc
     1021 gagcagataa acaagcgcaa taaagccaac gatccagccg aagatcccaa acacgcgccg
     1081 gcgcgccact tcgggcggaa cctcgatgga aaaatcgatg tccatcgccg cgctctcgcc
     1141 cgttcgctct tttccaccaa tcattaacag caattgaatc acgagcaaag taagcagagg
     1201 aatgctgacg gccagtggaa acagcttggt tttgaacgac caacccgttg ccgcaaaagt
     1261 cgcgtaagcg gctatggcga ttagaaaaat gcaaaaaaag agactgccct tatgcatgag
     1321 ctggttcccg caaacgttgc cgacgctgct gaatgtaggg ataagcgatg acgacgacca
     1381 tcagtatcat taaagctacg acgatcggat gcgtgagcca gccgaagccg taacgggtca
     1441 tggatatgaa cagataagtt tcaatcactt tgccgagaac aaaacccagg acaaagggcg
     1501 gccgcggcca accgaaaagc accataaagt aactgagcac gccgaaaatc aaagtgacaa
     1561 tcagatcggc aatttgcccg ttcgctgtat agctgccgac gaaaaccagc aacaaaataa
     1621 acggaataat cagtcccccg cgcacgtacg tcagtttcgc gagctgatcg agtatcgcta
     1681 gcgaaagaat gacggtgatc acgttggcaa tcgcaagggt ccaaaccatg gagaaggtga
     1741 gggaaaggtg cttagtcaac atttccggcc ccggcatgat gcccataatg agaaacccac
     1801 tgagcaggat tgccatggca ccgctccccg gcacaccgaa agcaaccgta ggaatcaact
     1861 cgccgccctc tttggaattg ttggcggcgc cgggacccaa gacaccgcga atatctcctt
     1921 tgccgaatcc gccgcgctcc ttcgaatccg tcgcgctctg aaccgcatgt gcatacgcca
     1981 tccattgcgc cacgccacca ccggcgccgg gcatgatgcc gacgaaaacg ccgataatcg
     2041 agcaacggaa aacaagccaa aaatgacgga aggtgtcttt gacgccttcc atcacgccgg
     2101 tgcttaaatt ttccggcggg cggtcgcccg caatggaagt gccgcggacc gccaaatcga
     2161 tgatttcagg aatcgcgaag acaccgacga gaacggggac aaagtcgagc ccgtcccaga
     2221 gatacatcat gccaagatcg aatcgaagcg agccggattg ccgttcctga ccgatggttg
     2281 agataagcag gccgaagagt cccatggcaa atcctttgat ctgcccgcgt gtgctggttc
     2341 cgctcaggga agtgatgcat gaaatcccaa tgagcgacag cataaaaagc tccggcgaac
     2401 cgaaggtgag caccagcggt cgaacgatcg gaacggcgag ggcgagcgca accgcgccaa
     2461 tcagggcacc caatagtgag ctcatcagcg cagcgccaag cgcgcggccg gcttcgcctt
     2521 tcttggccat cgggtaacca tcgacgactg tcgccgccga gatcgcctct ccgggcacgc
     2581 cgaagagcac cgacgtgata tcgcccgttg tcgcggcgac cgaatgcatc cctagcaaga
     2641 aagcgaacgc ctcggcggga ctcatcttga agatga
//
LOCUS       DASULZ010000848         3451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_910570, whole genome shotgun sequence.
ACCESSION   DASULZ010000848 DASULZ010000000
VERSION     DASULZ010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910570"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..1814
                     /locus_tag="VFY96_17420"
     CDS             18..1814
                     /locus_tag="VFY96_17420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX5608310.1"
                     /translation="MSSSGCSSISMPSMPSFSWRSGPKADATEEALFDEGMRSFNEKR
                     YVRAIDNFSKLRTDYPFSPQITQVELKIADAYYLNQQYPEAINAFKEYQSMHPTNENI
                     PFVLLRLGQSHFDQFTSTDRDQKNTEIAKGYFENVINSYPKSPQAVEAKQKLAKCLEY
                     LAEHEFNVAFFYFKQEKYPAARDRFEEIVRKYKDTPTAVKSLFYLGESYRNEKNVIRA
                     SLAYEALIEHYPETKFAADAKTQLASLDKEKRDPLALLLMRDHRPTAGPTPEVKEDPA
                     LAKLKDINLVAKTEVVHEEPGQEKGMMRRVADKLNPFSSSSSTKKEEKPSETVMETLA
                     KRGQAQKEAQKKDGKANDVVGQIDDALKQKGIDSASRETNLKPPAPDLPNTDDLAQAP
                     KPTDTAALLSSVDSSLKRSGKDGTELPPAPEPAEGFKNAAAAQANIAKTTQTQETPKD
                     VQSSGILSSIDQKLAARGVQPEQFEKPPTAEEVKTATIEKSRANNKFELEPKLPIEKG
                     PLFLNPGNVELGDKSAAMPETKEAETVTATPESPSRVLVKGPIRTDGNSIASETGTKR
                     PASSTDDEAKGVFDQLRQDVENASRALNPFRW"
     gene            complement(1783..2745)
                     /locus_tag="VFY96_17425"
     CDS             complement(1783..2745)
                     /locus_tag="VFY96_17425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5608311.1"
                     /translation="MKRDYWVLATVFGVLGAATLFARWWAAPDVPARSRLAKANPVTQ
                     APAQARPITVRLTGELSPLNAVDVKSTLAGRISAIRFKTGERVSANAIVAVIEPANRM
                     QSTGALEAALQSARDDRTKKQEQLAVSERELARAEEWVRENLIARRDLEQANAQADVA
                     RAQLRLAQARVAQQEAMLAQGRALERRARILAPIAGVVVHRWLDVGATVTEATAILRI
                     ADVNRLKLISRISARDVAEVRAGMTARLTAAGAPGKTFSGKVARVRRASDAPEETEIE
                     IEVTSDGALRPGMAVEATIDQTKLKKAGLKPAAATTSGKDSELC"
     gene            complement(2855..>3451)
                     /locus_tag="VFY96_17430"
     CDS             complement(2855..>3451)
                     /locus_tag="VFY96_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5608312.1"
                     /translation="WVLLALAVFVVYLVLGILYESFIHPLTILSGLPSAGLGALIALY
                     LFNMDLSIYAFVGLIMLIGIVKKNAIMMIDFALEGQRNDGKPPAQAIYEGALVRFRPI
                     MMTTMAAIFGALPIAIGWGAGAEARQPLGVAVVGGLVISQVLTLYITPVVYLYLEKCQ
                     EYFVTLPARLRASRGSTRRETAPARPPMGIESASEKLR"
BASE COUNT          860 a         1021 c          918 g          652 t
ORIGIN      
        1 gcctgttttg ccttgccatt tcgtcctccg gctgttcgtc gatttcgatg ccttccatgc
       61 cgtcgttttc gtggagaagc ggaccaaaag ctgacgctac tgaagaggct ttatttgatg
      121 agggcatgcg cagcttcaat gagaagcggt acgtgcgggc catcgacaac ttttccaaac
      181 tcagaaccga ttatcctttc agcccgcaaa tcacgcaggt cgaattgaaa attgccgacg
      241 cctactactt gaatcagcaa tatcccgaag ccatcaatgc attcaaagaa taccaatcga
      301 tgcatccgac gaacgaaaat attccgtttg tcttgctgcg cttggggcaa tcccacttcg
      361 accaattcac cagcacggac cgcgatcaga aaaacaccga aatcgccaag ggctatttcg
      421 aaaacgtcat taacagctac ccaaagtcac cccaggccgt cgaagcgaaa caaaaactgg
      481 caaagtgcct cgagtatttg gctgaacacg aattcaacgt cgcgtttttt tatttcaagc
      541 aagagaaata tcccgcggcg cgcgatcgct tcgaagaaat tgtgcgcaag tacaaagaca
      601 cccccacggc ggtcaagagt ctgttctacc tcggtgagag ctatcgaaac gagaaaaacg
      661 tgatccgcgc aagcctcgca tacgaagcgc tgatcgagca ctatccagag accaaattcg
      721 ccgcggatgc caagacgcaa ttggcttcac tcgataagga aaaacgcgat cctttagcgc
      781 tgctgctcat gcgcgaccat cgtccgacag cggggccaac gccggaggtg aaggaagatc
      841 ccgcgctcgc caagctaaag gacataaacc tggtagcgaa gacggaggtc gttcacgagg
      901 agcctggaca agagaaaggt atgatgcgac gcgtggccga caagctcaat cctttttcct
      961 ccagtagcag tacgaagaaa gaagagaaac caagcgaaac cgttatggag acgctcgcca
     1021 agcgtgggca agcgcaaaaa gaagcgcaaa agaaggacgg caaagccaat gatgtggtgg
     1081 gacagattga cgacgctttg aagcaaaagg gcatcgactc ggcaagtcgt gagaccaatc
     1141 tgaaaccgcc ggcgccggac ctgccgaaca ctgatgactt ggcccaagcg ccgaaaccga
     1201 cagatacggc cgcgctgctc agttcagtag attcgtccct caagcggagc ggaaaagatg
     1261 gcacagagct gccacccgca cctgagcctg cggagggatt caaaaacgct gctgcagccc
     1321 aagccaatat cgccaaaaca acacaaactc aggaaacgcc gaaagatgtg cagagcagtg
     1381 ggattctcag cagcatcgat cagaagctcg cagccagggg cgttcagccg gaacagtttg
     1441 agaagccgcc gacagcggaa gaggtcaaga cagcaactat cgagaaatcc cgggcgaata
     1501 ataaattcga actcgaaccc aagctcccca tcgagaaggg cccacttttt ttgaatcctg
     1561 ggaacgtcga gcttggcgac aaatccgctg ctatgcccga aaccaaggag gcagagacag
     1621 taactgctac acccgagtca ccgtcgcggg tgctcgtcaa gggcccgatc cgaacagacg
     1681 gtaattcaat cgcgagcgag acaggcacga aacggccggc ctcgagcacg gatgacgaag
     1741 cgaagggggt ttttgatcag ctccggcagg acgtcgagaa cgctagcaga gctctgaatc
     1801 ctttccgctg gtagtcgcag ctgcgggttt aagcccggct ttcttcagct ttgtttgatc
     1861 gatagtggcc tcgaccgcca tcccaggacg cagtgcgccg tcagaagtaa cttcgatttc
     1921 gatttcggtt tcttccggcg catccgacgc gcgtctaacg cgtgcgacct ttccggaaaa
     1981 cgtttttccc ggcgcgcccg cagcggtgag acgcgccgtc attcccgcgc gaacctcggc
     2041 gacatcccgt gcagatattc tggagattaa ctttaatcga ttgacgtcgg cgatcctcag
     2101 aatcgccgta gcttcagtaa ccgttgcacc gacatcaagc cagcggtgaa ccaccacccc
     2161 ggctatcgga gcaagtatcc gcgcccgacg ctcgagcgcg cgaccctggg ccagcatcgc
     2221 ttcttgctgg gcaactcgcg cttgcgctaa tcgaagttgt gcgcgcgcca catccgcctg
     2281 cgcattcgct tgctccaaat cacgtctggc gatcaagttt tcgcgtaccc attcctcggc
     2341 acgcgccagt tcccgttcac tgacggcgag ttgttcttgc tttttcgtcc gatcgtctcg
     2401 agcgctctgc agcgccgctt cgagtgcccc agtgctttgc atccgattgg cgggctcaat
     2461 aaccgccacg atcgcgttcg cgctcacgcg ctcgccggtc ttaaagcgta ttgcgcttat
     2521 cctgccggcg agagtggact tcacatcgac cgcgttcagg ggtgaaagct cgccagtcag
     2581 tcgtactgtg atcggtctgg cctgggccgg ggcttgcgtc actggattgg cctttgccaa
     2641 cctgctgcgc gccggaacat caggcgcggc ccaccagcgt gcaaaaaggg ttgcagcgcc
     2701 caaaacaccg aaaaccgttg cgagcaccca ataatcacgc ttcatatgcg cgaccgagct
     2761 atcatcttgc gatccctcgg agctgaaaaa gaaatcgggc taccggctgt tggcccggta
     2821 gcccgactac tgacaatacc tgctggccaa ccgcttaacg cagtttctcc gaagcggact
     2881 cgatccccat tggcggcctc gccggagctg tttctcttcg tgtactgccc ctggaagcac
     2941 gtagccttgc aggaagcgta acgaagtact cctggcactt ctcgagatag aggtagacca
     3001 cgggcgtaat gtagagggtc agaacctgcg agatgaccaa accgcccacg actgcgacgc
     3061 cgagcggttg gcgcgcttcg gcgcccgcgc cccacccgat ggcgatcggc aaggcgccaa
     3121 aaatcgccgc catggtcgtc atcatgatag ggcggaagcg cactaatgcg ccctcgtata
     3181 tcgcctgcgc cggcggtttc ccgtcgttac gttggccttc caatgcgaag tcgatcatca
     3241 tgatcgcgtt cttcttgacg atgccaatca gcatgatcaa accgacaaac gcgtaaatgc
     3301 tgaggtccat attgaaaaga tacagagcga tcagcgcccc cagacccgcc gacggcaagc
     3361 ccgaaagaat cgtcagagga tgaataaaac tctcatacag aattcccaag accagataaa
     3421 cgacaaaaac cgcgagcgcc aatagcaccc a
//
LOCUS       DASULZ010000849         4869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_707015, whole genome shotgun sequence.
ACCESSION   DASULZ010000849 DASULZ010000000
VERSION     DASULZ010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707015"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="VFY96_17435"
     CDS             complement(<1..1031)
                     /locus_tag="VFY96_17435"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX5608313.1"
                     /translation="MLLINNQEVEKLFTMQDCLAALEDGYDDLLKGDAVFRPRLDLWM
                     PCDRPDGYYRWGTMEGASRKIGVFAIRMKSDVVYWPNEQTEEKYCVQPGTWCGLVMVF
                     SVRNGEPLAIINDGLIQHMRVGACAGLGAKYLAREDACDVGIVGSGGMARSYLEAFYE
                     VRKLKHVSVYSPTKANRDKFAREMTGKLDVEVVAVDTPEKVVREADIIATCTDSTQTV
                     FDDPEWLKEGAHITSVRACEIGPRVIKRCDLSVKLGRNTIEAMDEGMIRLHGNVGYIA
                     GQPEERARIPNPKVDNYKGDYFKYFMDVRAGKVPGRISDKQVTFFINVGTQGLQFAAC
                     AGKIIELAK"
     gene            1238..2149
                     /locus_tag="VFY96_17440"
     CDS             1238..2149
                     /locus_tag="VFY96_17440"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX5608314.1"
                     /translation="MKILRFNDDRIGVIKDGNRVVDVSDSVSARKAKGPQRVMEEIIE
                     GWRKYRRRFEKILAEQSGVPVDSVEIHSPLARPGKFLAAFSNYIDRPERTADNFPNEY
                     FYKSPDLVGPGESVELRDIPACVVYQPEAEFAFVIGKTAENVPQNKALDYVFGYVPVF
                     DISTRGLVRRTQFIGKGQGSHGCCGPWIITKDEIADPHNVVVKSWTNGEPRQNYNTSA
                     MAHKLPNQIAWLTKFVRLCPGDIVATGTFHEGLKPVNSGDTIEIEFENMGRAKFNVKG
                     NSPRKDVEWLPGKNQPTPPPGGGIHLV"
     gene            complement(2210..2977)
                     /locus_tag="VFY96_17445"
     CDS             complement(2210..2977)
                     /locus_tag="VFY96_17445"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5608315.1"
                     /translation="MRWNKVRGILFLIALIAIWEIASRSKLLNPLIMPAPARIFGVFS
                     DLIFSGQIPWQILVSMKRAAAGYLLAAAVFIPLGIVMGLSQHIYCLFEVAVEMLRPVP
                     PPVVIPVAMLFFGLEDEMKIFVIFFSCAWPILLNTLDGVRSVDWVLLNTARTFGLSRL
                     KTIRQVVLPAASPQIMTGLRVSLPITLILVVISEMVGSTDGIGYFILDSQRRFKVAQM
                     YAGMLSLAILGYTLNQLFNLLHRGLMSWHWGMMKRKE"
     gene            complement(2979..3758)
                     /locus_tag="VFY96_17450"
     CDS             complement(2979..3758)
                     /locus_tag="VFY96_17450"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5608316.1"
                     /translation="MNALKQFNWSGWLFFGLLLLAWELGARGVPKLQLYFPPLSRVLV
                     ALIASVESGALVSQFVITLGRFLEGYLLSAAIGVVLGVVLGYFRLAHELFETLIEFLR
                     PMPSVAIIPVAILMLGISDSMIVAVTVYATVWPILINTIDGVRHIEQVLIQTGRTFGL
                     SKAGILWQVILPAASPYIVTGLRIGLSIALILVTTAEMVAGSKGLGFFILDEERSMNS
                     GNMYAGIIVVAALGYALNRLFLALEAKVLKWRHGMRARQAG"
     gene            complement(3911..4612)
                     /locus_tag="VFY96_17455"
     CDS             complement(3911..4612)
                     /locus_tag="VFY96_17455"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014689317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5608317.1"
                     /translation="MAIGDITCAVEAGEFVSFVGPSGCGKTTLLMSIAGLIAPSAGRV
                     IVKGKEVAGPPSNLVLLFQEYNKSLFAWRTVLGNVRFGLEARGNHSADAIKKAQALLE
                     LVGLGGFEKHYPWELSGGMQQRVAIARALAYEPEVLLMDEPFGSLDALTRLELEDSLL
                     ALWAELKTTIIFITHDIEEAIYLSSRIWVLSRRPSKIIDEMAIDFPRPRNQVNTRAEP
                     RFMEIRNDIYRNIRN"
BASE COUNT         1111 a         1454 c         1321 g          983 t
ORIGIN      
        1 tgcttcgcca gttcgatgat tttgcctgcg caggcagcga attgcaggcc ctgcgtcccc
       61 acattgatga aaaacgtaac ctgtttgtcg ctgattcggc cgggaacctt gccggccctg
      121 acgtccatga agtatttgaa atagtcgcct ttgtaattat cgaccttggg gttgggaatg
      181 cgtgcccgtt cctccggttg cccggcgata tagccgacat tgccgtgcaa acgaatcatg
      241 ccctcgtcca tcgcttcgat ggtatttctg ccaagcttga ccgagagatc gcagcgcttg
      301 attacccgcg ggccgatctc acaggcgcgc accgacgtga tgtgcgcccc ttccttcaac
      361 cactcaggat cgtcgaacac cgtctgggtc gaatccgtgc aagtggcaat gatatcggct
      421 tcgcgcacga ctttttccgg cgtgtccacc gcaacgactt cgacgtcgag cttgccggtc
      481 atttctctgg cgaatttgtc gcgattggcc tttgtcgggc tgtagacgct cacatgtttg
      541 agcttgcgca cttcgtagaa cgcttccaag tagctccgcg ccatcccgcc ggaaccgaca
      601 attccgacgt cgcaagcgtc ctcccgcgcc aggtacttcg ccccgaggcc cgcgcaggca
      661 cccacccgca tgtgctggat cagcccatcg ttgatgatcg ccagcggctc gccgttgcgc
      721 acgctgaaga ccatcaccaa cccgcaccac gtgcccggct gaacgcaata tttttcttcg
      781 gtttgttcat tgggccagta aaccacgtcg gatttcatcc ggatcgcgaa cacgccgatt
      841 ttgcggctcg ccccttccat ggtgccccat cggtagtagc catcgggccg gtcgcagggc
      901 atccatagat cgagccgcgg ccgaaaaacc gcgtcgccct tgagcaaatc gtcatagccg
      961 tcttcgagcg ccgcgagaca atcttgcatc gtaaaaagct tttcgacttc ctggttgttg
     1021 atcagcagca tgagttcctc ctcggattga attacaacaa cggtggtcgt tttaaaagct
     1081 gccgaggggg taaggcccga taatctgacc actcgatcgg gccagaggag tcaagcgctt
     1141 cggcatcgcg aactctttgc aagatgcacg agcgatttga catcatcctg gggttatcgc
     1201 aaagataggc ggaatttcgc cccgagagga gagaacattg aaaattctgc gattcaacga
     1261 tgacaggatc ggcgtcatca aagatggcaa tagggtcgtc gatgtgagcg acagcgtgag
     1321 cgcgcgcaaa gccaaaggac cacagcgcgt catggaagag atcatcgaag gttggcgcaa
     1381 atatcgccgc cgctttgaaa aaatcctcgc cgagcagagc ggtgttcccg tggacagcgt
     1441 cgaaatccac tcaccgctgg cgagacccgg caagtttctc gccgctttca gcaactacat
     1501 cgatcgtccg gagcgcaccg ccgacaattt tcccaatgaa tatttttaca agtcgcccga
     1561 tctcgtcggc ccgggcgaat cggtcgagct gcgcgacatt cccgcctgcg tggtctatca
     1621 gcccgaagcc gagttcgcct ttgtcatcgg caaaaccgcg gaaaacgtgc cgcaaaataa
     1681 agcgctcgat tatgtcttcg gctatgtgcc ggtgttcgat atctccactc ggggcttggt
     1741 gcgccgcact cagtttatcg gcaaaggcca agggagccac ggctgctgtg ggccgtggat
     1801 catcaccaaa gacgagatcg ccgatccaca caatgtggtg gtgaaatcat ggaccaacgg
     1861 cgagccgcgg cagaactaca acaccagtgc catggcgcat aagctcccga accagatcgc
     1921 atggctcact aagttcgtcc gcctctgtcc gggcgatatc gtcgccacag gaacttttca
     1981 cgaagggctc aagccggtga actccggcga taccatcgaa atcgaatttg aaaacatggg
     2041 gcgcgccaag ttcaacgtca aaggcaacag cccgcgcaaa gacgtggaat ggctccctgg
     2101 caagaatcaa cccacaccgc ctcccggcgg cggcatccat ctggtataaa gaatggagta
     2161 cggagtgatg ggtactgggg tcttatcact gcaccattcc attattccat cactcctttc
     2221 ttttcatcat cccccagtgc catgacatca gcccgcgatg tagtaaattg aagagctgat
     2281 tgagcgtgta gccgagaatc gcgagcgaca acatccccgc atacatctgc gcgactttaa
     2341 atcggcgctg ggagtcgaga atgaaataac cgatgccgtc ggtgctgccg accatttccg
     2401 agatcacgac gagaatcaaa gtgatcggca ggctcacccg caatccggtc atgatctgcg
     2461 gcgaagccgc cgggagaacc acctggcgaa tagtttttag tcgtgacaag ccgaaggtcc
     2521 gcgccgtgtt gagcagcacc cagtccacgc tccggacgcc gtccagcgta ttcagcagaa
     2581 tcggccaggc gcaggaaaag aaaatcacga agatcttcat ttcgtcttcc aggccgaaaa
     2641 aaagcatcgc caccgggatc accaccgggg gcggcaccgg ccgaagcatt tccacggcga
     2701 cttcaaacag gcaatagata tgctgcgaca gtcccatcac aatgccgagc gggatgaaca
     2761 ctgcggcagc gagcaggtaa cccgccgcgg cgcgcttcat gctgacgaga atttgccagg
     2821 gaatttgccc ggagaaaatg agatcggaga agacgccgaa aatccgcgcc ggcgccggca
     2881 tgatcagcgg attgagcagc ttgcttcggg aagcgatctc ccaaatcgcg atcagcgcga
     2941 tgagaaagag aatgccgcga actttgttcc aacgcatttt atcccgcctg gcgcgcccgc
     3001 atgccatgac gccacttgag cacttttgct tcgagcgcca aaaacaaccg attgagcgca
     3061 tagccaagcg cggccaccac gataatgcct gcgtacatgt tgccgctgtt catcgagcgc
     3121 tcttcatcca gaataaaaaa ccccaatcct ttgctgccgg ccaccatctc cgccgtcgtc
     3181 accaggatga gcgcgatgga aagaccgata cgcaggccgg tcacgatgta cggcgacgcc
     3241 gcgggcaaaa tcacctgcca caggattccg gctttgctga gaccaaaggt ccgcccggtc
     3301 tgaatcagca cctgctcgat atggcgcacg ccgtcaatgg tattgatcaa gatcggccag
     3361 acggtggcat agacggtgac ggcaacgatc atggaatcgc tgatgccgag catcaatatc
     3421 gcgaccggaa tgatcgccac cgacggcatc ggccggagaa attcaattaa agtctcaaag
     3481 agctcatgag ccaggcgaaa atagcccagc acaaccccga gcaccacgcc gatggccgct
     3541 gataagagat agccttcgag gaagcgaccg agggtgatca caaactggct aacgagcgcg
     3601 cccgactcta ccgatgctat cagcgcaacg agcacccggc tcaggggcgg gaagtacaac
     3661 tgtagtttgg ggacaccccg cgcccccagt tcccaggcga gcaataagag cccgaagaac
     3721 agccaacccg accagttgaa ttgcttgagc gcgttcacgg tctacttgcg ccgcgttcga
     3781 tcccgcagca tccccgcatt cccgcatgtc ttaggcggga agcaagatcg gatcctggtt
     3841 cccgataaga gcattccggg gtgaccttcc caaatgggtt cattcccact ggcgctattc
     3901 cgcaccgctt tcaattacgg atattgcggt aaatatcgtt tcttatttcc atgaagcgcg
     3961 gctcggctcg cgtgttcact tgattgcgcg ggcggggaaa atctatcgcc atttcgtcga
     4021 taatctttga cggccggcgg ctcaacaccc agatacggct ggaaagatag atcgcctcct
     4081 cgatgtcgtg cgtgatgaaa atgattgtcg ttttgagctc cgcccagagc gccagcaacg
     4141 aatcttccag ctccaaacgg gtcaaggcgt cgagcgagcc gaagggctcg tccatcaata
     4201 gcacctccgg ctcgtaggcg agcgctcgcg cgatggcgac ccgctgctgc atacccccgg
     4261 agagctccca cggataatgc ttttcgaatc cgccgagtcc caccaactcg agcagcgcct
     4321 gcgctttttt aatcgcatct gcggaatgat tgccgcgtgc ttccaatccg aagcggacgt
     4381 tcccgagcac cgtgcgccag gcgaacaggg atttgttgta ctcctggaac agcaggacca
     4441 gattggatgg cgggcccgcc acttctttgc ctttgacgat tacgcggccg gcactaggtg
     4501 cgatcagacc ggcgatcgac atcaataaag tcgttttgcc gcagcccgag ggaccgacga
     4561 acgaaacgaa ttcgccggct tcgactgcac aggtaatgtc gccaatggcc atcgtgcttt
     4621 caccgctcgc ctggcggtaa gttttcgata gcccttggac ctcaaggatt ggcacgggag
     4681 aaacaatggg agcggagtgc tggagtgatg gagtaataaa gtgttgcacg aactaatgac
     4741 ccagcactcc agtactctat catcccagcg cctttttcac gccttcggcg ctaactcgct
     4801 gaccacgtcc tgcgctttga tgcctttggc gatcagcttg tacttttgcg tgagatcgat
     4861 ggtcgcctg
//
LOCUS       DASULZ010000850         2519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_99134, whole genome shotgun sequence.
ACCESSION   DASULZ010000850 DASULZ010000000
VERSION     DASULZ010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99134"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="VFY96_17460"
     CDS             complement(<1..1000)
                     /locus_tag="VFY96_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX5608318.1"
                     /translation="MDRAKKVFKNLSGIEIRELYTPRDLNEFDHEGDLNRPGEFPFTR
                     GVYPNMYRGKLWTMRQFAGFASATETNRRYRYLLDQGTTGLSVAFDMPTLMGYDSDHP
                     RGKGEVGRCGVAIDSVADMETLFHNIPLDQVSVSMTINGPAIVLFAMYLVVAERQGVS
                     FTKLRGTLQNDILKEYIAQKEWISPPAPSLKLINDIVRFALESTPLYHPISISGYHIR
                     EAGSSAVQELAFTLYDGLTYVKSAIESGLRVDDFAPRLSFFFNAHNDFFEEIAKYRAA
                     RRLWAREMKNRFAVKDPASLALRFHAQTAGCALTAQQPYNNVVRVALQALAAILGGA"
     gene            complement(975..2153)
                     /locus_tag="VFY96_17465"
     CDS             complement(975..2153)
                     /locus_tag="VFY96_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5608319.1"
                     /translation="MDFELTEEHRMVQEMTRSFAERKLKAVASRMDHDGTYPAELVKE
                     LGQLGLMGVFAPQEYGGAGMEFVSYIIALEEISKVWASVGTIMSVNNSLVCGPLLRYG
                     SEGQIKRYLPLIASGEWTGCYALTEPMSGSDAGSIQTQAKRVSDGYLLNGTKVFTTNG
                     SRAELAIVYAVTDPSRGKQGISAFLVERPSPGFTVGRIEDKLGLRASETAQLVFENCH
                     ISAENLLGREGEGFKIALETLDGGRIGIAAQALGIAQGCLDQSLDYSRERRQFGQRIA
                     DFQAIQGMLADMATEIDCARLLTHRAAWLYQQRKKVTAAAAMAKLFASEACNRAAYKA
                     LQIHGGYGFIKDFAVERFFRDARVTTLYEGTSEIQRLVIARQLIQEGAPSDGSRQESF
                     "
     gene            complement(2294..>2519)
                     /locus_tag="VFY96_17470"
     CDS             complement(2294..>2519)
                     /locus_tag="VFY96_17470"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397003.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX5608320.1"
                     /translation="FAVVSIAVNRELGLDPAKVNVRGGAVALGHPIGASGARILTTLI
                     YALKDLDLKRGVASLCIGGGEAVALMVERE"
BASE COUNT          522 a          753 c          722 g          522 t
ORIGIN      
        1 gcgctccgcc gagaatcgcg gcgagcgcct ggagcgcgac gcgcacgacg ttgttatagg
       61 gctgttgagc ggtgagcgcg cagccggcgg tctgtgcgtg aaagcggagc gccagcgagg
      121 cgggatcttt caccgcgaag cgatttttca tctcccgcgc ccacaggcgg cgcgccgcgc
      181 gatattttgc gatctcttcg aaaaaatcgt tgtgcgcgtt aaagaaaaaa gagaggcgcg
      241 gcgcgaagtc gtccacgcgc aaccccgact cgatcgcgga tttgacgtag gtcagaccat
      301 cgtaaagcgt gaacgcgagc tcctgcaccg cgctcgagcc ggcttcccgg atatggtagc
      361 cgctgatgct gatcggatga tacagcggcg tcgactccag cgcgaagcgc acgatgtcat
      421 tgatgagctt aagggacggc gccggcggcg agatccattc cttttgcgcg atgtattcct
      481 tcaaaatatc gttctgcagc gttccgcgca gtttcgtaaa gctcactccc tgccgctcgg
      541 caacaacgag atacatcgca aataatacaa tcgccgggcc attgatggtc atcgacacgc
      601 ttacctgatc caagggaatg ttgtgaaaga gcgtttccat gtcggcgacc gagtcgatgg
      661 cgacgccgca tctgcccact tctcctttgc cgcgcgggtg gtccgagtcg tagcccatga
      721 gcgtaggcat atcgaaggca acggacaaac cggtcgtccc ttggtcgagc agataacggt
      781 aacgccggtt agtttctgtg gcgctggcaa atccggcgaa ctggcgcatc gtccaaagtt
      841 tgccacggta catgttcgga taaacgccgc gggtgaaggg aaactcaccg gggcgattta
      901 ggtcgccctc gtgatcaaat tcgttgagat cacggggcgt gtagagctct cggatttcga
      961 tgcccgagag attcttaaaa actttcttgg cgcgatccat ccgaaggcgc tccttcctgg
     1021 atcagttggc gcgcgatgac caggcgctgg atctccgacg tcccttcata gagcgtggtg
     1081 acgcgcgcgt cgcgaaaaaa ccgctccacg gcaaagtcct tgataaagcc gtagccgccg
     1141 tggatctgca gcgccttata ggcggcgcgg ttgcacgctt ccgaagcaaa caacttggcc
     1201 atggccgctg ccgcggtcac ttttttccgc tgttggtaga gccaggcggc gcgatgagtg
     1261 agcagccgcg cgcaatcgat ctccgttgcc atatcggcga gcatgccctg gatcgcttga
     1321 aaatcggcga ttcgctgacc gaactgccgg cgctcgcggc tatagtcgag cgactgatcc
     1381 agacaccctt gggcgatgcc aagagcctgc gctgcgatcc cgatccggcc gccgtcaagg
     1441 gtttcgagcg cgatcttgaa cccctcgccc tcgcgtccga gtaaattttc cgcggaaatg
     1501 tgacaattct caaagacgag ctgtgcagtt tccgaagcgc gcagaccgag cttgtcttca
     1561 atcctaccga cggtaaatcc gggcgatggc cgctcgacca gaaaagccga tatgccttgc
     1621 ttgccgcggc tcggatcggt gaccgcgtaa acgatcgcga gctcggcgcg gctgccgttg
     1681 gtggtgaaaa ctttggtgcc gttcagaaga taaccgtctg acacacgctt ggcctgggtt
     1741 tggatcgaac cggcgtctga accggacatg ggttcggtca atgcgtaaca gccggtccac
     1801 tctccactgg caatcaatgg caaatatcgc ttgatctgcc cttcgctgcc gtagcgcagg
     1861 agcggaccgc ataccagcga attgttcacg ctcatgatgg tgccaaccga ggcccacacc
     1921 ttagagattt cttccaatgc gatgatgtag gagacaaact ccattcccgc gccaccgtac
     1981 tcctgcggcg caaaaacgcc catcaacccg agctgaccga gctctttcac caactccgct
     2041 ggataagtac cgtcgtgatc catgcgtgag gcgacggcct tgagctttcg ctccgcgaag
     2101 ctgcgcgtca tctcttgaac cattcgatgt tcttcggtca gctcaaaatc catgttgatt
     2161 tttgatccgt tcaaaatgtt tcactgctgc gctccgttca accgctttgc tacgttccaa
     2221 ccgtcgggac ctccgaaccg gcgctgaacg atttgaactg cttgaacggc tggagccccg
     2281 ggtaaagcgc gagtcattcc cgctccacca tcagcgctac cgcctctccg ccgccgatgc
     2341 acaagcttgc gactccgcgc ttcagatcca aatctttgag cgcataaatc agtgtcgtga
     2401 gaatccgcgc gccgctggcg ccgatgggat ggcccaaagc gacggcgccg cctcggacat
     2461 tgactttagc cggatcgagg ccgagttctc gattgacagc aatggaaacg actgcaaaa
//
LOCUS       DASULZ010000851         2935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1111551, whole genome shotgun sequence.
ACCESSION   DASULZ010000851 DASULZ010000000
VERSION     DASULZ010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111551"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1320)
                     /gene="dnaA"
                     /locus_tag="VFY96_17475"
     CDS             complement(<1..1320)
                     /gene="dnaA"
                     /locus_tag="VFY96_17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX5608321.1"
                     /translation="MPELWTQAVAEIKERIGRQNYETWIKPIGFVSRNKNEICLDVPN
                     KFFRDWLTEHYLSQIQDILSTLAKHDVKVVFEINEKTNRQANAEKGAKREERERVQKS
                     NTNLVPKYTFQNFVVGASNQFAHAACVAVANQPGEHYNPLFIYGGVGLGKTHLVNAIG
                     HQSAQRSGLKVVYLSSESFMNELIGSLRRDKMDEFKKKFRNVDILILDDVQFIAGKER
                     TQEEFFHTFNSLYESHKQIVITSDKFPKEIPGIEDRLRNRFEWGLIADIQPPDMETRV
                     AILQKKAENEGVQLPHDVAFFLASNIDSNVRELEGSLTRLGAFSSLTKATITVDLAKD
                     VLRNTLKNAQQEITVENIQKTICDYFNVKIGDLKAKRRTQNIAVPRQVAMYLCRKYTE
                     TSFPAIGDKFGGRDHSTVIHASKTIERKIKEDPHMQTTIEKLERNLNI"
     gene            1699..>2935
                     /gene="hemL"
                     /locus_tag="VFY96_17480"
     CDS             1699..>2935
                     /gene="hemL"
                     /locus_tag="VFY96_17480"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX5608322.1"
                     /translation="MTATAKIFLRATRRIPGGVNSPVRAWKSVGGVPLFITRGRGSYV
                     FDASGKRYLDFVGSWGPLILGHAHPKVVQVLRRRALQGFTFGAPTILEVELAEAVAAA
                     VPSIEKLRLVSSGTEATMSAIRLARAFTGRSKIIKFDGCYHGHSDGLLVKAGSGIATL
                     SLPESAGVPRSFARETLTAKYNDSQYLQKIFARYKNEVAAVIVEPICGNMGVIKPEPD
                     FLPSLRQITRENGALLIFDEVITGFRVAYGGAQALYDVRPDLTCLGKILGGGLPLAGF
                     GGRSEIMDLLAPLGPVYQAGTLSGNPLAVAAGLATIKLLSRSGTYDKLEAQGGYIEEG
                     MREIIANSRLEATINRVGSMMTIFFGIHRVSHPDEARNCDRHKFVEFFHAMLDRRIYL
                     PPAPFEAMFISLAHSKNDLV"
BASE COUNT          673 a          722 c          772 g          768 t
ORIGIN      
        1 gatgttcaga ttgcgttcaa gcttttcgat cgtggtttgc atgtgtggat cctctttgat
       61 ttttctctcg atcgtcttag aagcgtgaat aacagtggag tgatcgcgcc cgccaaattt
      121 atcgccgatg gcgggaaagg aagtttcggt gtacttccgg cagagataca tggcgacttg
      181 acgcggcacg gcgatatttt gtgtgcgccg tttggctttg agatcgccga tcttaacatt
      241 gaaatagtcg cagatggttt tctgaatatt ttcgaccgtg atctcctgct gggcgttttt
      301 cagtgtgttt cgcagaacgt ctttcgcgag gtccactgtg atcgtcgctt tggtcaacga
      361 agaaaacgcg ccaaggcggg tgagagatcc ttcgagctca cggacattcg agtcgatgtt
      421 cgacgcgagg aaaaaggcca cgtcatgggg cagttgaaca ccttcgtttt cggctttctt
      481 ctgcaaaatc gcgacgcggg tctccatatc tgggggctgg atgtcggcga tcaaacccca
      541 ctcgaagcga tttcttaaac ggtcttcgat gcccgggatt tcctttggaa atttatctga
      601 agtaatgacg atttgtttat gcgactcgta aagggaattg aaggtgtgaa agaactcctc
      661 ctgagtacgc tccttgccgg cgataaattg gacgtcgtcg agaataagaa tatcgacgtt
      721 tctgaatttt ttcttgaact cgtccatctt gtcgcgccga agggatccga tcagctcgtt
      781 cataaatgat tcagagctta gatagacaac ttttagaccc gaacgctgtg ctgactggtg
      841 accgattgcg ttgacgagat gggtcttacc caggccgaca ccaccataaa tgaacagagg
      901 gttataatgc tcgcccggtt ggttcgcgac cgcgacacat gccgcgtgag cgaattgatt
      961 actcgcccct acaacgaagt tttggaaggt atacttcgga acgagattgg tgttactttt
     1021 ctgtactcgc tcgcgctctt ctcgcttcgc gcccttttca gcgttcgcct gccgattagt
     1081 cttttcgtta atttcaaaaa caactttgac gtcgtgcttt gcgagcgtgc tcaggatatc
     1141 ttggatttgg gacaaataat gttccgtcaa ccagtcgcga aaaaacttgt ttggcacatc
     1201 gagacaaatt tcatttttgt ttcgtgagac aaagccgatc ggtttgatcc aggtctcgta
     1261 attctgcctg ccgatccgtt ctttgatctc cgcaaccgcc tgcgtccaca gctctggcat
     1321 aaaattacac taaggccttg aaaccctgac aagaaatgac gaaatagtta caaacaaagt
     1381 tatccacagc tgttgataac tctggaagcg ccgcagggga gtgaaaccaa aagggcagcg
     1441 ccctccagga tcgagaaaag gagaattttt tattaacacg agttgaaaac agttctgccg
     1501 ccaggcacta gcgtagctca ttttcctcgt gctttctcgc aaaccggtaa aaaattttcg
     1561 cgctggagaa tcaggaaaaa tttttaatag tccttttcaa atttctttgc aacaaaaaaa
     1621 gtgcggcgaa attttttttc tgccgcgatt tttttcttga cagcttgcgc gacgaatgtt
     1681 agcgacgcaa cccaaaccat gacggcaacg gcaaagattt ttctccgcgc cactcgtaga
     1741 attcccggcg gtgtcaatag tccggtgcga gcatggaagt cggtcggcgg tgtgccactc
     1801 tttattacgc gcggcagagg cagctatgtt tttgatgcca gcggaaagcg ctacctcgat
     1861 ttcgttggct catggggacc gctgatcctg ggtcacgctc acccgaaggt cgttcaagtc
     1921 cttcgacggc gagcgctgca aggctttacg ttcggcgcac ctacaatact ggaggtcgaa
     1981 ctcgcggaag ctgtcgcggc cgccgtgccc tccatcgaaa aactccgtct cgtaagttca
     2041 ggcacagaag caacgatgag cgccatccgg ctcgcgcggg ccttcacggg ccgaagcaag
     2101 ataattaagt tcgacggctg ctatcatgga cactccgatg gcttgctggt caaagccggt
     2161 tccggcatag caaccctcag tttgccggag agtgccggcg ttccgcgatc cttcgctcgc
     2221 gaaacactga cagctaaata caatgacagt cagtacttac aaaagatttt cgctcgctac
     2281 aaaaatgagg tggccgcggt tatcgttgag cctatttgcg gcaatatggg tgtgattaag
     2341 ccagagcccg atttcttacc gagtttgcgc cagattacgc gtgagaatgg tgcattgctg
     2401 atttttgatg aggtcatcac gggatttcgc gtcgcatacg gcggcgcgca agcgctttac
     2461 gacgttcgtc cggatttgac gtgtttggga aaaattctcg gcggcggttt gccgctggca
     2521 ggcttcggcg gccgcagtga gataatggac ctgcttgctc ccttgggacc ggtctatcaa
     2581 gcgggaacgc tttcgggaaa tcctttggca gtggcggcag gattagccac aatcaagttg
     2641 ctcagtcgct ccggtacgta tgataagctc gaagcgcagg gcgggtacat agaagaagga
     2701 atgcgtgaga ttattgccaa ctcgcggctt gaggcgacga tcaatcgggt cggatcgatg
     2761 atgaccatat tcttcggcat ccaccgggtg agccatccgg atgaagctcg caactgcgat
     2821 cgccataagt tcgtcgaatt tttccacgcc atgctcgacc gccgcattta tcttccgccg
     2881 gcgccatttg aagctatgtt catttcgctc gcccattcga agaatgattt ggtaa
//
LOCUS       DASULZ010000852         4278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_504667, whole genome shotgun sequence.
ACCESSION   DASULZ010000852 DASULZ010000000
VERSION     DASULZ010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504667"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..650
                     /locus_tag="VFY96_17485"
     CDS             255..650
                     /locus_tag="VFY96_17485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608323.1"
                     /translation="MTKLLSGTKSLFPEFPIDFDPDLISHTLEYRGCGIRAASYELRA
                     QSWLPEACVWLHTEDGHRKLWVHSFAHCFAAEELTFQNKVEADHWALEAAKSIIDRAF
                     EIAPLAVTARWANGRLRSITALARRAIPS"
     gene            798..1787
                     /locus_tag="VFY96_17490"
     CDS             798..1787
                     /locus_tag="VFY96_17490"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5608324.1"
                     /translation="MDLQELSQWMKDRNLKGHWEHSEWAQTVKPFLWKGDEIMQALHW
                     AGELITTGEAGRRTIQMRNPGLVAGMTNTVHISVQLVRPGEIAAAHRHTAAAIRFIIK
                     GTPDAYTIVEGERFPMFEGDFITTPNWTWHDHFNNSTEPVMWLDGLDVRLVTHFGAMI
                     QENFSKQQQPIEKPENFSSKIFSHARPTWIKNSFQAPPYRYSWEETHASLEALKESAG
                     DPYDGVILEYANPVDGGHTLPTCSCSIQLLRPNERTKRHRHTSTTVYYAFRGQGMTKV
                     SANELNWSKGDIFVVPSWQWHSHENATHADAILFSINDQPSTEALGLYREEAE"
     gene            complement(1995..3062)
                     /locus_tag="VFY96_17495"
     CDS             complement(1995..3062)
                     /locus_tag="VFY96_17495"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5608325.1"
                     /translation="MAQFVIDADGHIMEDHKDIFAHIKGNFSEMDWHSTWPMFDADGW
                     QRGLARKGKREDPNAEAWVRFQDEHGIDCAVLYPTSALALGMIQLPAWASALAQGYND
                     WLYDRFISQSPRLKGVALLAPQDPEAAAAELRRCVKDLGFCAGLLPSVTNNRTPLYGS
                     PVFHEIYDEAQRLDVPLTIHGGVSQNLGLDRLCSFLEAHMLEHPVGLFLQITNMMFQG
                     VFQEFPKLRVAYLEAGAGWVPFMMDRMEEDYEKFAARLAPHLKDPPSKFFKSGNIFVT
                     MEVEERTAPYLPDLIRDDVIMWASDFPHERERDQFGGDLPHLYARKDVSEEFKRKMLY
                     DNPVRFYRFSEGDVGAKRKKL"
     gene            complement(3175..4182)
                     /locus_tag="VFY96_17500"
     CDS             complement(3175..4182)
                     /locus_tag="VFY96_17500"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5608326.1"
                     /translation="MRKMSALALVWLLLFSVRFGFAQSAQTVAAAKKEGGKIILYTSM
                     ETFTADALKKAFEAKTGLQMEYWRGGSTEILDRVLSEHRVGKPQFDVVATTGDHMRLM
                     AKGGAFGKYQSPSFKGFAKDAIDPELGARYRNVLYGVIYDKNQIKPAEAPKTLEDVVK
                     PEYRGKLVMPHPTGHTLTTQWLASLDKIMAKPRAEKFIRDLAAAKPIFAESIVPAADR
                     VGTGETPIGITFVRFVLTYNKQGANLDYVRDYTMLGDGQFITIGAKPAHPTAGKAFID
                     FFLDEESMKIQADTGEFVNRKGVYPPLADADKIKFVQMYSFSKEDFEAKKKEYQKIFF
                     Q"
BASE COUNT          983 a         1115 c         1189 g          991 t
ORIGIN      
        1 agcatcttgg gcttcgaaga agattgccgg tcggctatct gcttgccaac atacgccgtt
       61 gaacaacagt tgcgctcatt gggggtcaag acggtcgtgt gcatgtgaaa cgatgaaaga
      121 cagcgaagaa agaaatttgt accgcgcata aagcgagcta tccagttttc gcagcgcaat
      181 cggcgagttc ttccgtcgga gtgcgtgaat ttacccaacc cgtctggtat atggtttgcg
      241 cagtctgccc ctcgatgacg aaattgctca gcggcacgaa atcattattt ccagagttcc
      301 cgatagactt cgatcccgac ctgatcagtc atacgctgga atatcgcggc tgcggtatca
      361 gagccgcttc atacgaactc cgggcacagt cctggctgcc ggaggcgtgc gtctggctgc
      421 atacggaaga tggccaccga aaactgtggg ttcatagctt tgcccattgc tttgctgccg
      481 aggaacttac ttttcagaac aaagttgagg cggatcattg ggctctggag gcggcaaaat
      541 cgatcatcga tcgcgcgttc gagatagcgc ccttggcagt aactgcccgt tgggccaacg
      601 ggcgactgcg ctcgatcacc gctttggcac ggcgcgctat tccgtcttga accgaagtcg
      661 ctcgacggca tttcgatcag accacggacg gatttacctc taagttccct actctcaaaa
      721 aaacctccag cttgacagcg ttctcaccgg tttgtattga gaagtcacgg ctttgcgcct
      781 tcacgcccgg aggaactatg gatttacaag agctcagtca gtggatgaaa gaccgcaatc
      841 taaaaggcca ttgggaacac tcggaatggg cccaaacggt gaagcctttt ttgtggaaag
      901 gcgacgagat catgcaggcg ctgcattggg cgggtgaatt gattaccact ggggaggcgg
      961 gccgccgaac gatccagatg cgtaaccccg gtctggtcgc gggcatgacc aacacggtgc
     1021 atatatctgt gcaactggtg cgacctggag agattgccgc ggcgcaccga cacacagccg
     1081 cagcgattcg ctttatcatc aaaggcactc cagacgccta cacgatcgtc gagggcgaac
     1141 gctttccgat gttcgaaggg gatttcatta cgacacccaa ctggacctgg cacgaccact
     1201 tcaacaattc cacggaacca gtgatgtggt tggatggctt ggacgttcgg ttggtgacgc
     1261 acttcggtgc gatgatccag gagaatttca gcaaacagca gcagccgatc gagaagccgg
     1321 aaaatttttc gtcaaaaatt ttctctcatg cgcgcccgac ctggatcaaa aattcttttc
     1381 aagcgccacc gtaccgatat tcgtgggaag aaacccatgc gagcctggag gcgctcaagg
     1441 aaagcgcggg agatccgtac gacggtgtta tcttggaata tgccaacccc gtggatggcg
     1501 gtcatacgct cccaacctgt tcctgttcca tccagctact gcggcccaac gaaagaacca
     1561 agaggcaccg tcacacgagc acgacggttt attatgcatt tcgtggtcaa ggcatgacca
     1621 aggtctctgc gaacgagctc aattggagca aaggcgacat ttttgtcgtg ccttcctggc
     1681 aatggcattc gcacgagaac gcgactcatg cagacgcgat tctcttttcg atcaatgatc
     1741 agccgtcgac ggaagcgctg ggactgtatc gagaagaagc ggaatgatga agaaagatcg
     1801 aggcatggat gatagaggat cgaattttgc gcattcgcga cagagttcag cgcgaacaga
     1861 cggagggaag gctccaatcg atggaacggc cactggactt atccgtttca gctgagccgg
     1921 tcgagcattg ctaattttta tccagtggtg ctcgccgcgc gcgaggcgag ttagctcctg
     1981 gcgatctcct cgcattaaag cttctttctt tttgcgccga catcgccttc gctgaagcga
     2041 tagaatctca ccgggttatc gtagagcatc tttctcttga attcttctga tacgtcttta
     2101 cgggcataga ggtgcggcag gtcgccgccg aattgatcgc gttcgcgctc gtgtggaaaa
     2161 tcggaagccc acatgatcac gtcgtcgcgg atcaaatctg gaagatatgg agcggtgcgc
     2221 tcttcgactt ccatggtgac aaaaatattt ccggatttaa aaaatttgct cggcggatcc
     2281 ttcaagtggg gggccagccg cgctgcaaat ttttcgtagt cttcttccat gcgatccatc
     2341 atgaacggaa cccaaccggc gccggcttcg agataggcga cccggagttt gggaaattcc
     2401 tgaaaaacgc cttgaaacat catattggta atttgcaaaa acagaccaac cgggtgctcg
     2461 agcatgtggg cttccaaaaa actgcaaagg cggtcgaggc cgagattctg gctcacgccg
     2521 ccatgaatcg tgagcggcac gtccaaccgc tgagcttcgt cgtagatttc atgaaacacc
     2581 ggcgagccgt aaaggggcgt gcgattgttc gttacggaag gtagaagccc agcgcaaaac
     2641 ccgagatctt tgacacagcg gcgcaactct gcggcagcgg cttcgggatc ctgaggcgcc
     2701 aataatgcaa cccccttcaa gcggggtgat tgactgataa accgatcata aagccagtcg
     2761 ttgtagccct gcgcaagcgc cgaggcccag gcgggcagct gaatcatccc gagtgccagc
     2821 gcggaggtcg gataaagaac cgcacaatcg atgccgtgtt cgtcctgaaa acgcacccat
     2881 gcttcggcat tgggatcttc gcgcttgcct ttgcgcgcca agccgcgctg ccaaccatcg
     2941 gcatcgaaca tcggccaggt ggaatgccaa tccatttcac tgaagttgcc tttgatatgc
     3001 gcaaagatgt ctttgtgatc ttccatgatg tggccgtctg cgtcgataac gaattgtgcc
     3061 atgcttatta ctcctgataa cgttgtcgct agcattctga ggcgccgctg aagaatctcg
     3121 ccaagatcct tcacttcgtt caaaatgaca gatacgcaat ttcaatgcac gtggttattg
     3181 gaaaaagatt ttctgatatt cctttttttt cgcttcgaaa tcttccttgc tgaagctgta
     3241 catctgcacg aatttgatct tatcggcatc ggcgaggggc gggtagacgc ccttgcggtt
     3301 gacgaattct ccggtgtcgg cctgaatctt catgctttct tcgtcgagga agaagtcgat
     3361 aaaagccttg cccgcggtag ggtgggccgg cttggcgccg atggtgatga actggccatc
     3421 gccgagcatc gtgtagtcac gcacataatc gaggttggcg ccttgtttgt tgtaggtcaa
     3481 gacgaagcgc acgaaggtga tgccgatggg ggtttcgccg gtgccgacgc ggtctgcagc
     3541 ggggacgatc gattcggcga aaatcggttt ggcggcggcc agatcacgga taaacttttc
     3601 cgcacgcggc ttagccatga tcttgtccaa gctggcgagc cactgggtgg tcaaggtatg
     3661 gccggtggga tgcggcatca cgagtttgcc tctgtattca ggcttgacca catcctcgag
     3721 cgtcttggga gcttcggcag gcttgatctg attcttgtcg taaatcacac cgtaaagaac
     3781 gttgcggtaa cgagctccca gctccggatc gatggcgtct ttagcaaagc ctttgaacga
     3841 cggcgattga tacttgccaa aggcacctcc tttagccatc agccgcatat gatcgccggt
     3901 ggtcgcgaca acgtcgaatt gaggtttgcc gactcggtgc tcactcagaa ctcggtcgag
     3961 aatttccgtc gagccgccgc gccaatattc catttgcagc ccggttttgg cttcgaaggc
     4021 ttttttcaat gcgtcggctg tgaaggtttc catcgaagta taaaggatga ttttgccacc
     4081 ctcttttttt gcagcggcga cggtttgcgc cgattgggcg aagccgaacc gtacactgaa
     4141 aagcagcaac cagaccaatg ccagagccga cattttgcgc attgagctcg atcctttcat
     4201 tgaagggcgt gcagtttttt gacttatagt gtacgcccca agtgagagtc aaggcggatt
     4261 ggccgagacc agggcgca
//
LOCUS       DASULZ010000853         3299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1111653, whole genome shotgun sequence.
ACCESSION   DASULZ010000853 DASULZ010000000
VERSION     DASULZ010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111653"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VFY96_17505"
     CDS             complement(<1..700)
                     /locus_tag="VFY96_17505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608327.1"
                     /translation="MKNLFCTLLHSLMLFFVVNSYADFTGKGHVHTVAESTETFLNAG
                     CRINDTCDLKRFALTTLAHEVWFSDDPKNPTHANGVIMEYETESVDAIEKYAIVQFVK
                     GCVFSTTKNRDGKIDKQINYGVSSFGESIPLCFPRWVIDSQDADPVYNSDPEFGRFYL
                     LRWNAPGSYADRTQKYYGAQKPNLPVVYMTDYPAGAFVAGSGVKNTSLQFKTCIYKAS
                     EVPAQTVRDNVNFAN"
     gene            823..1677
                     /locus_tag="VFY96_17510"
     CDS             823..1677
                     /locus_tag="VFY96_17510"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5608328.1"
                     /translation="MASIAFALCAAALYGSADFLGGLAARRSAVMPVMIFSQLAGSCM
                     LIAVLPWLPTGDATIADWLWGTAAGIALAIGLTLLYQALAVGKMSLVAPVTAVLAVIF
                     AGFVGLLGGDHLSVAGLAGIALALAAIILISQDGAPQSPPHDHETRSSRILPTALSAG
                     LLIGVFYAALKQSSASSGLLPLLSARLTALIVLAVVALSCRSSLCVGRDAIWLILLGG
                     TLDIMANVLYVFAARHGMLTIAATLTSLYPASTIILARFVLGERLRAIQIAGLSCAGV
                     GVVLIGAA"
     gene            complement(1786..2436)
                     /locus_tag="VFY96_17515"
     CDS             complement(1786..2436)
                     /locus_tag="VFY96_17515"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEX5608329.1"
                     /translation="MKNVFAVRLHNLLAVGVLLCAGVFSVSFVEAADSPRVNDAYALR
                     TDMNEFGVWAGGSPGNSQFIGNIEDRQLLLFALRYGRVLGTWESVALEYTFDIFPAAV
                     YFEPSNVRRGSSTIYGGGLSPIGFKLNFAPQSWIQPFVAASVGFLYFTEDVPVPDSSR
                     FNFTPEFGVGLQFFFAPKRALMLGYKLHHISNANTGDSNPGMDSHVIYAGMSFFIP"
     gene            complement(2532..>3299)
                     /locus_tag="VFY96_17520"
     CDS             complement(2532..>3299)
                     /locus_tag="VFY96_17520"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX5608330.1"
                     /translation="QKIFDDNKADGIVITHGTNTIEETAYFLNLTLKTDKPVVVVGSM
                     RPASAISADGYLNLVNAIKVAADPNSCGRGCLLVMNDTIFNGRDVTKNATYRVEAFQS
                     RDLGPLGYADGDGKIVYYHQPVRKHTTATEFDVRNLQSLPRVDMVLSYVGADGTMIEA
                     AANAGAKGIVSAGTGAGRPTPAEDAAFDKCFKEKGMIMCLCSRVASGRVVRSPGLQKR
                     GFVAGDNLQPWKARLLLSLALTKTTNADDIQRMFDTY"
BASE COUNT          680 a          901 c          924 g          794 t
ORIGIN      
        1 gattcgcgaa gttcacgttg tctcttacgg tttgcgctgg cacttcgctg gccttgtaaa
       61 tacaagtttt gaactgcagt gaggtgttct tgaccccgct cccggctacg aacgctccgg
      121 cgggataatc cgtcatgtaa accaccggaa gattcggctt ttgcgctccg tagtattttt
      181 gcgtgcgatc ggcgtatgag cccggtgcat tccagcgcaa caagtagaat ctgccgaatt
      241 caggatcgct gttgtatacc ggatccgcat cttgtgagtc gatcacccag cgtggaaagc
      301 agagtggaat actttctccg aagcttgaaa cgccgtaatt gatctgcttg tcgattttgc
      361 cgtcgcggtt tttcgtcgtg gagaaaacgc agcctttgac gaattgcaca atcgcatatt
      421 tttcgatcgc atcgacggat tccgtctcgt actccataat aacgccattt gcatgggtcg
      481 ggtttttggg atcatcgctg aaccagacct cgtgagccaa agtggtcaga gcgaagcgtt
      541 tcagatcgca agtgtcgtta atgcggcagc cggcattcaa gaaagtttcc gtcgattctg
      601 cgacagtatg gacgtgtccc ttgccggtga aatcggcgta ggaatttacc acgaagaaga
      661 gcatgagcga gtggagtaga gtgcaaaaaa gatttttcat ggagaactcg gcgctccaat
      721 cgaatgacat gaaggcgccg tcatcaacca gagatcgttt tccctcgcca cacttccgct
      781 tgctgtttac ttacacgaag cgggagttaa tttaaatccc acatggcgtc aattgcgttc
      841 gcactatgcg cggcggcgct ctacggcagc gccgattttc tcggcggttt ggctgcacgg
      901 cgttccgcgg tgatgccggt gatgattttt tctcagctcg ccggttcctg tatgctcatt
      961 gccgtattgc cgtggctgcc gacgggtgac gccacgatcg ctgattggct ttggggcaca
     1021 gcggcgggca tagccctagc gattggattg acactgcttt accaagcttt ggcggtcggc
     1081 aaaatgagtc ttgtggcgcc cgtgaccgct gtcttggccg tgattttcgc cggattcgtg
     1141 ggactgttgg gcggcgacca tttgtcagtt gccggattgg ccgggatagc gcttgcgctg
     1201 gccgcgatta tattgatcag ccaagatggc gcgccgcaaa gccctcccca cgaccacgag
     1261 actcgctctt cgcgtatcct gcccactgcg ctttccgccg ggctcctgat cggagttttt
     1321 tatgcggccc tcaaacagag cagcgcgtct tcgggtctgt taccgctgct gtcagcgcgc
     1381 ctcacggcgc tgatcgtgct cgcggtcgtt gcgttgtcgt gccgatcatc gctatgcgtc
     1441 gggcgcgacg cgatttggct gattctgctc ggtggcacgc tcgacataat ggccaacgtg
     1501 ctttacgttt ttgccgcgcg ccacggtatg ctgacgatcg ccgcgacatt gacgtcgctc
     1561 tatccggcaa gcaccatcat tctcgcccgg tttgttctgg gcgagcgtct ccgggccatc
     1621 cagatcgccg ggctctcgtg cgccggagtt ggagtcgtgc tgattggagc ggcttaacgc
     1681 cgccactttt tttttgacct aagctcggtt ttgtcccaaa tcgccaccta gccgacgatg
     1741 gccgtcggcg gcattgaaag tgagatgtcg cgcaatccgc gagctctatg gaataaaaaa
     1801 agacattccc gcgtagataa cgtgtgagtc catgccagga ttgctatctc cggtattcgc
     1861 attggagatg tggtgcagct tgtaacccaa catcaatgct cgtttcggcg caaagaaaaa
     1921 ctgcaggccg acaccgaact caggggtgaa gttgaaccgt gaggagtcgg gaacgggtac
     1981 atcttccgtg aagtagagaa atccgacact ggcagcaacg aacggttgta tccaactctg
     2041 tggggcgaag tttagcttga atccgatcgg cgagagaccc cctccataaa tggtcgagct
     2101 tcctcttcgc acattgcttg gttcgaaata aacggccgcg ggaaaaatat cgaacgtgta
     2161 ttcaagagcg acggattccc atgtgcccaa aacccgtcca taccggagcg cgaacaagag
     2221 cagttgtcgg tcctcgatgt tgccaataaa ctgcgaattg ccgggcgacc cgcccgccca
     2281 gacgccgaac tcattcatat cggttctaag cgcgtatgcg tcgtttactc tcggggagtc
     2341 tgccgcctcg acaaacgaga cgctaaaaac accagcgcaa agtaaaactc cgaccgccaa
     2401 caaattgtgc agccgaacag caaagacgtt cttcatattt aactccctgg gcattcggtc
     2461 tacacgaatc ataagccggc cgtgttgctt tgaacccgaa ggtcgacgcg agtgatctgc
     2521 gcatgattat ttcagtaggt gtcaaacatc cgttggatgt catcggcgtt ggtcgtctta
     2581 gtcagcgcca acgagagtag cagccgtgct ttccagggtt gcaaattgtc accggccacg
     2641 aaaccgcgtt tctgcagacc aggacttcta acgacccggc cggaagcgac gcggctgcac
     2701 agacacataa tcatgccttt ttccttgaag catttgtcga acgcagcgtc ttccgcagga
     2761 gttggccggc cggcgcctgt accggcgctg acgatcccct tcgcgccagc attcgctgcg
     2821 gcttcgatca tcgtgccgtc ggcacccaca taggagagca ccatgtcgac gcgcggcagc
     2881 gactgcaaat tgcgcacgtc gaattcggtt gcggtcgtgt gtttgcgcac aggctgatgg
     2941 tagtagacga tcttgccgtc gccgtcggcg taaccgagcg gtccgagatc gcgagactga
     3001 aatgcttcca cgcggtaggt agcatttttg gtcacatcgc gaccattgaa gatggtgtcg
     3061 ttcattacca gcaagcagcc ccgtccgcat gaattgggat cggcggcgac tttgatcgcg
     3121 ttcaccaggt tcaaataacc atcggcgctg atcgccgacg cgggacgcat ggagccgacg
     3181 accacgacgg gtttatcggt cttgagtgtt agattgagaa agtaagccgt ttcttcaatc
     3241 gtgtttgtgc cgtgcgtaat gacgatgcca tccgctttgt tgtcgtcgaa aatcttctg
//
LOCUS       DASULZ010000854        10662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1419097, whole genome shotgun sequence.
ACCESSION   DASULZ010000854 DASULZ010000000
VERSION     DASULZ010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 10662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10662
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419097"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1506)
                     /locus_tag="VFY96_17525"
     CDS             complement(<1..1506)
                     /locus_tag="VFY96_17525"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5608331.1"
                     /translation="MAEPINISSLPYVEELYRKFLSEPSSLPDEWRHFFTQPGPDNGT
                     AAPRAKPVNLQSHEQLVNGPSAAPSAPVLSSQLNELIYSYRAYGHLRAQIDPLKRLNL
                     PNVAPVNLRSEVFGFTDAHMDKLFASESIQLSGPLTLRELIGRLERIYCGPIGFEFMH
                     LGDRLEREWLQQRIEQIENRSVFDPDEQKQILSRLIEAQAFEQFIRRKFLGAKSFSLE
                     GAESLIPLLEVAIGKAAEQGILEIVMAMAHRGRLNVLANIVRKPARDIFREFIDPSVA
                     HADGSGDVKYHLGHSHDYVTATGQRVHLSLCFNPSHLDFVNPVAMGRVRAKQDRVGDL
                     ERQQALALLVHGDAAFAGEGITQETLNLSRLPGYEIGGTLHVVVNNQIGFTTPPRVAR
                     SSVYATDVAKILQLPIFHVNGDDPEAVAYVVRLASDFRRTFRQDAVIDLYCYRRLGHN
                     EGDEPSFTQPRMYQAITKHPTVREIYSKRLLSLGRVTREETAAAEKNYLTHL"
     gene            1859..3193
                     /locus_tag="VFY96_17530"
     CDS             1859..3193
                     /locus_tag="VFY96_17530"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5608332.1"
                     /translation="MDDEPLPRKNIARVLEGEDFHVYEAESGDQAIDAIGERDFDLIL
                     SDLKIPGTDGLGVLRHVRSVSPQTIFILMTGYASVDSAIEAFRGGAHDYILKPVVLED
                     VLQKVKRLLEAKSLAWEVELLRRQVNCNSESTGLIGESKVIRDILSMISKIAPTSSNV
                     LITGESGVGKEIVARAIHLQSPRKDKVFLPVNCSAIPETLLESQLFGYMKGAFTGAAS
                     SQEGLFQRAQGGTIFLDEIGEMPLSLQPKILRVIEDKEILPIGSTHPAKLNVRIIAAT
                     NRELQSEVAEGKFREDLFYRFNVINIRVPPLRERREDIPLLAEHLVRRHNAEMKKAYK
                     GVANSALKLLMSLPWKGNVRELDNVLELAMILGNGEWITPAELPQCTTPLEEDGTEPM
                     NNLRIAVQSYEKSHIENVLKETAGDKTRAAELLGVSRSSLYRKMESLGIRNE"
     gene            3311..3961
                     /locus_tag="VFY96_17535"
     CDS             3311..3961
                     /locus_tag="VFY96_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX5608333.1"
                     /translation="MAQIFHPSTNTISRVTIFGGLILLIAVVTGLTTINESPYITEVG
                     VARTQPVPFSHKHHVGDDGIDCRYCHTSVEESSFAGIPPTKTCMNCHTQIWPDSPILE
                     PVRESFRTGKSIQWTRVHNLPGFVYFNHSIHVHKGIGCVTCHGRVDRMPLMWRENTLY
                     MDWCLDCHRAPERFVRPREYVFSMEYQAPADQLALGQKLVKQYRIQKLTSCSTCHR"
     gene            3972..7031
                     /locus_tag="VFY96_17540"
     CDS             3972..7031
                     /locus_tag="VFY96_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HEX5608334.1"
                     /translation="MSNGMDPAGLLGIGQQSAGGREYWRSLEELAGTEAFKDLIGNHM
                     PRHAAAFTSAMDRREFLKLMGASLALAGISGCLSPAPAPPDEKIVPYVNQPEALVPGK
                     PLYFATAIPADGYGRGVLVESHMGRPTKVEGNPQHPASLGAADAVTQASVLGLYDPDR
                     SRVISNAGRVATWDAFFSALNAELESQRVTQGAGLRILTETVTSPTLAAQLQEILRTF
                     PKAKWHQFDGGGGDYAHQGARMAFGQTFETLYRLEEADVIVSLDADFLLRSPASLRYA
                     REFINRRRVPRASDEANRLYMIESTFSLTGSNADHRLAIRPSAFPQLARTLAARVGVQ
                     IAAAQPAISPAQEKWLGALVKDLQAHRGRSAIMPGEYQPAIVHALAHSMNDALGNIGK
                     TVFYTDAVAVNPTNHAESLRELVRDLEDNAAQVLIILGGNPAYTAPAELDVAGHLVTS
                     NFNVHLSLYEDETSAVCHWHIPQAHYLESWSDVRAYDGTVTIMQPLIAPLYGGRTAHE
                     ILAAVAGQAERSSYEIVHDYWRAQSKTKNFDAAWRTALHDGFIAGSAFSPRNVRPKPA
                     ASFDTSEPQRPSTAAAAKTPNAPSLEVIYRPDPSIIDGRFANNGWLQELPKPLTKLTW
                     DNAVLMSSATAGKLGLTYQVGSTGGEHGTIYADVVELEYDGRRTRGPAWIVPGHADDC
                     VTIHLGYGRRRAGRIGTNVGFNANALRTLETLWHNASIAIRKTDGQYALACTQFHHLM
                     EGRDLVRAGTIEQYRKNPKFLIERESEKEAPATLYPGYKYDGYAWGMSIDVNACIGCN
                     ACVAACHAENNGPVVGKQEVLRGREMHWIRVDRYHRGEPSNPETYFQPVPCMHCENAP
                     CELVCPVGATTHSQDGLNDMVYNRCVGTRYCSNNCPYKVRRFNFFLYSDFKTESLKPL
                     RNPDVTVRSRGVMEKCTYCVQRINQAKIAAEKEERTVRDGEIEPACQQACPTRAIIFG
                     NINDPQSRVSQLKAQPLSYGLLTELNTRPRTTYQAKLRNPNPEIENG"
     gene            7033..8406
                     /gene="nrfD"
                     /locus_tag="VFY96_17545"
     CDS             7033..8406
                     /gene="nrfD"
                     /locus_tag="VFY96_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX5608335.1"
                     /translation="MASKPSDVSGDSLPVLEPGHTFASITDKISSIVLTGRTPLFWYI
                     SFGIGFLLVLLLLFCITALVSVGIGLFGVMIPVAWGWAIVNFVWWIGIGHAGTLISAI
                     LLLLRQKWRQSINRFAEAMTLFAVACAGIFPLIHLGRPWFAYWLFPYPSTMGIQPQFR
                     SPLVWDVFAVSTYFTVSLLFWFLGLIPDLATLRDRSTRRVPRFIYGMLAMGWRGSAVH
                     WHRYESAYLLLAGLATPLVVSVHTVVSFDFAVALVPGWHSTIFPPYFVAGAIYSGFAM
                     VLTIAIPLRKLYGLEDFITLRHLKNMGIVMLATGMIVAYGYLMETFMAWYSGDLFEKY
                     MMMNRMFGPYGWLYWLLILFNILIPQALWVRSLQTNPWVLFFIAIAVNIGMWLERYVI
                     VVVSLHRDFMPSAWGMYSGTIFDYGVFVGTIGLFVALLFLFIRLLPMISIFEMRELVH
                     EEEGRGE"
     gene            8478..8987
                     /locus_tag="VFY96_17550"
     CDS             8478..8987
                     /locus_tag="VFY96_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEX5608336.1"
                     /translation="MAEFDSPEALLEASRRAFAEGFRRMDAYSPFPVDGLAEAIGFPR
                     TRVPLIVLIGGIVGCLGGFWLQYWVSVIDYPINIGGRPLNSWPSFIPVTFELTILLAA
                     LAAFFGVLGLNRLPMPYHPVFNVDRFELASRNRFFLCIEAADNKFELDRTRRFLDEIG
                     SLGTYEVEP"
     gene            9076..9585
                     /locus_tag="VFY96_17555"
     CDS             9076..9585
                     /locus_tag="VFY96_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5608337.1"
                     /translation="MADQPRYEPLQKSDLFDDQRASRPLVEGTVPRGQLNADAEFYTG
                     KVNDEPAKTFPFTVDRELLLRGQERFTIFCSPCHSRLGDGQGMIVRRGFRPPPSFHIE
                     RLREAPPGHFFDVISHGFGAMPDYAEQIPPHDRWAIAAYIRALQLSQSAALTDVPETE
                     RRALLEEKK"
     gene            9582..>10662
                     /locus_tag="VFY96_17560"
     CDS             9582..>10662
                     /locus_tag="VFY96_17560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608338.1"
                     /translation="MNGLDVTLSTELDPLRTKSLIAGGLALVLCFIGWFFNRGEFFRA
                     YLIAYVFWIGIPLGCLGILMIHHLVGGTWGFVIQRCLEAAVRTFPIMFLLFLPLLLGL
                     PDLFVWARRDVISADPVLQEKAAYLNMPFFFVRAVVYFAVWFAIGFFLTKWSQAQERS
                     ADPAFTQRLQTLSGPGLVLYGLTVTFSAIDWLMSLEPHWYSTIFGMIFMVSHGLIALT
                     FVIGAAYFLSRREALRQIVAPWVLQDLGNLLLAFVMLWAYLSFSQFLLIWVENLQHEI
                     PWYLHRLTGGWAVIGIALIVVQFALPFVLLLSRAVKRNPAALAAVAGGVAAMHFIELF
                     WFVAPTFHPDGFSLHWTTIMAPVGIG"
BASE COUNT         2163 a         2963 c         3097 g         2439 t
ORIGIN      
        1 gagatgcgtc aaataatttt tttccgccgc ggcagtttct tccctggtga cccggcctag
       61 tgaaagcagc cgtttgctgt aaatctcgcg cacggtcggg tgtttcgtga tggcttggta
      121 catgcgcggc tgcgtaaagc tcggctcatc tccttcgttg tgaccgagcc tgcgataaca
      181 gtagagatcg atcactgcgt cctggcggaa tgtcctgcgg aaatccgacg ccagccgcac
      241 cacgtaggcc accgcttcgg ggtcgtcgcc gttgacgtga aaaatcggta actgcaatat
      301 ttttgcgaca tcggtagcat aaaccgaaga gcgcgccacc cggggcggtg tggtgaaccc
      361 gatttgatta ttgaccacga cgtgcagagt gccgccaatt tcataaccag gcaggcggct
      421 caaattcaaa gtttcctggg tgattccctc gccggcgaag gcggcatcgc cgtgaaccaa
      481 taatgccaag gcctgttgcc gttccaggtc gccgacgcgg tcctgtttgg ctcgaactcg
      541 ccccatcgcc actggattga cgaagtccag atgactcgga ttgaagcata aggagagatg
      601 gacacgctgc cctgtcgccg tgacataatc atggctgtgc ccaagatgat atttgacgtc
      661 gccgctgccg tcggcgtgag ccaccgatgg atcgatgaat tcacggaaga tgtcacgggc
      721 cggtttgcgc acaatgttcg caagcacgtt cagccggccc cggtgcgcca tggccatgac
      781 gatttccaag ataccttgtt ccgccgcttt accgatcgcg acctccagca gcggaatgag
      841 cgactcggcg ccttcaagtg agaagctctt cgcgccgaga aatttcctgc ggataaattg
      901 ctcaaaggct tgcgcttcaa tgagccggct gagaatctgt ttttgctcgt caggatcgaa
      961 aaccgagcgg ttttcgatct gctcgatgcg ctgctgcaac cattcgcgct caaggcgatc
     1021 gccgaggtgc atgaattcaa agccgatcgg tccgcagtaa atccgttcaa gacggccgat
     1081 cagttcgcgc agtgttaacg ggccgctcag ctggatggat tccgaagcaa acagtttatc
     1141 catgtgagcg tcggtgaagc caaaaacctc agatctcagg ttcacaggtg cgacattcgg
     1201 aaggttcaga cgcttcaatg gatcgatctg cgcgcgtaaa tggccgtatg cacgatagct
     1261 gtaaatcaac tcattgagtt ggctcgagag aaccggagct gaaggcgctg cggacggtcc
     1321 gtttaccagc tgttcatgcg attgcaagtt gacgggcttc gctcgcggtg cagcggtacc
     1381 attatcgggc cccggttgcg tgaaaaaatg ccgccattcg tcgggcagcg aagagggctc
     1441 cgacaaaaac ttccgataca gctcttcgac atacggcaag ctggaaatat tgataggctc
     1501 ggccatcgga tttcgtttgt catcgttcgc tgtacaaagt tcgtgccgat aactctcgcg
     1561 gagtagtttt ttcttacatc gtccggatcg attcttacgc ccagtaactc agtgtcaggt
     1621 tcgcgggttg atattgaaac tagtcctgac gtccgaaaac taggacacaa aatcgctagc
     1681 tgtcctgttt ctgcgacggc gccgtgctgg gcaaggagct ttatctttcg tgagccaacg
     1741 gcacacttcc ctggcacaat gcttgccctg ccaacttgct ggtttgctgc atacgccagt
     1801 cgtggtctca acgtgctgga cattcattcg gggaacagtg agttcaagta ttctactagt
     1861 ggatgatgag ccgttgccgc gcaaaaatat tgcgcgcgtg ctcgagggcg aagactttca
     1921 cgtctatgaa gcggaaagcg gcgaccaggc gatcgacgcg attggcgagc gcgatttcga
     1981 tctgatcttg agcgatctaa aaatccccgg caccgacgga ctcggcgtct tgcggcatgt
     2041 gcgcagcgtt tcgccccaga ccatcttcat tctgatgacg ggttacgcat ccgtcgactc
     2101 ggccatcgag gcttttcgcg ggggcgcgca cgactatatc ctcaagccag tggttctcga
     2161 agatgtgctg cagaaggtca agcggttgtt ggaggcgaag agcctcgcct gggaggtgga
     2221 gttgttgcgc cggcaagtga actgcaactc cgaatccacc ggactgatcg gcgaaagcaa
     2281 ggtgatccga gacattcttt caatgatctc caagatcgcg ccgaccagtt ccaacgtgtt
     2341 gatcacaggc gaaagcggag tggggaaaga gattgtcgcg cgcgcgattc atctgcaaag
     2401 tccgcgcaag gacaaagttt tcttgccggt caattgcagc gcgatcccgg aaacgctgct
     2461 cgagagtcag ctttttggct atatgaaagg cgcgttcacc ggcgcggcga gctcgcagga
     2521 aggcttgttc caacgcgctc agggcgggac gatttttctc gacgagatcg gtgaaatgcc
     2581 gctcagcctg cagccgaaaa tattacgggt gatcgaagac aaagaaattc tgccgattgg
     2641 ctcgactcat ccagcaaaac tgaacgtgcg cataatcgcg gcgactaatc gcgagctgca
     2701 atccgaggtc gcggaaggaa aatttcgcga ggacttgttt taccgattta atgtcataaa
     2761 catacgcgtg ccgccgctgc gtgagcggcg cgaagatatt cctttgttgg ctgagcatct
     2821 ggtgcggcgc cataacgcgg agatgaaaaa ggcctacaag ggcgtggcca attcggcgct
     2881 aaaattgctg atgtcattgc cctggaaagg caatgtgcgc gaattggata acgtgctcga
     2941 gctggcgatg atcctcggga acggcgagtg gattaccccg gccgaactgc cgcagtgcac
     3001 gactccgttg gaagaagacg gcacggagcc gatgaacaac cttcggattg ctgtccagtc
     3061 ctacgaaaag agccatattg aaaacgttct aaaagaaacg gctggcgaca aaactcgcgc
     3121 tgccgagctt ttgggcgtca gccgttcctc tctctatcga aaaatggaga gcttggggat
     3181 tcgtaacgag tagcgcgatc cgcgctgcac cgctgaggat tacttcctct ctgggcgccg
     3241 acgacgcgac gagaactggt ctcctgcgct cgttgtgtgt ggtgtcgctg aagcagcttt
     3301 gtaaacgccg atggcgcaaa tttttcaccc cagcaccaac acgatctcgc gggtcacgat
     3361 tttcggcggt cttattctcc tgatcgcagt ggtgaccgga ctcacgacta tcaatgaatc
     3421 gccctacatc accgaagtgg gagtggcgcg cacgcagcct gtgcccttca gccacaaaca
     3481 tcacgtcggc gacgatggaa tcgattgccg ctattgtcac acttcggtcg aggaaagctc
     3541 gttcgccggt attccgccga cgaaaacctg catgaactgc catacccaga tttggccaga
     3601 cagtccaatt ctggaacccg tgcgcgaaag ctttcgtacc gggaaatcaa ttcagtggac
     3661 gcgcgtgcac aatctgccgg gatttgtcta tttcaaccac agcattcacg tgcacaaagg
     3721 catcggttgt gtgacctgcc acgggcgagt ggatcggatg ccgctcatgt ggcgtgagaa
     3781 cacgctatat atggattggt gtctcgactg tcatcgcgcg ccggaacggt tcgtacgccc
     3841 gcgcgagtac gtgttcagca tggaatacca agcgccggcc gatcagctcg ccttgggtca
     3901 gaagctggtc aaacaatatc gcatccaaaa gctgacgagt tgttcgacgt gtcatcgcta
     3961 ggagggagcc aatgagcaac gggatggatc cggcagggct gcttggaatc gggcagcaaa
     4021 gtgctggggg acgagagtac tggcgctccc tcgaagagct cgctggaacc gaagcgttca
     4081 aagatctcat cggcaatcac atgccgcgcc acgctgctgc cttcacgagc gctatggatc
     4141 ggcgcgaatt tctcaagctc atgggcgcgt cgctcgccct agcgggaatc agcggctgct
     4201 tgagtccggc acccgcgccg ccggatgaaa aaatcgttcc ctacgtcaat cagccggaag
     4261 cgctcgtgcc gggtaaaccg ctttatttcg cgaccgccat accggccgac ggctacggcc
     4321 gcggcgttct ggtcgaaagc cacatgggac ggccgacaaa agtcgaaggc aacccgcagc
     4381 atccggcaag cctcggcgcc gccgacgctg tgacgcaggc ttcggtgctc gggctttacg
     4441 atcccgaccg ctcgcgcgtg atcagcaatg cgggtcgggt ggcgacctgg gatgcgttct
     4501 tttcggccct caacgcggag ctcgaatcgc agcgggttac tcaaggcgcg ggtctgcgga
     4561 ttttgacgga aacggtgacc tcgccgacat tggcggcgca gcttcaagaa attcttcgca
     4621 cctttccgaa agcgaaatgg catcagtttg acggcggggg cggagattac gcgcatcaag
     4681 gggcgcgcat ggccttcggt caaactttcg aaacgcttta ccggttggaa gaggctgacg
     4741 tcatcgtctc gcttgatgcc gatttcttgc tgcgcagtcc ggcaagtctg cgttatgcgc
     4801 gggagttcat caaccgccgg cgggtccccc gggcatccga cgaggcgaat cgactctata
     4861 tgatcgaaag cacattttcc ttgacgggct ccaacgccga tcaccggctc gcgatcagac
     4921 caagcgcttt tccacaactg gccagaacgc tcgctgcgcg cgtgggcgta cagatcgcgg
     4981 cggcgcagcc cgcgatttca ccggctcaag agaaatggct gggtgcattg gtcaaggatc
     5041 tacaagcgca tcgcggccga agcgcaatca tgcccggcga gtaccagcct gctatcgtac
     5101 acgcgctggc acattcgatg aatgacgcac tcgggaatat cggcaagacg gttttttaca
     5161 ccgacgcagt ggccgtcaac ccaacgaatc atgcggagtc tctccgcgag ctggtgcggg
     5221 acctggagga taatgcggcg caagtgctga tcatactagg tggaaatccc gcctatacgg
     5281 cgcccgccga gctcgatgtc gccggccatt tggtcacgtc gaacttcaac gtccatctct
     5341 cgctttacga ggacgaaaca tccgccgtgt gtcattggca catcccgcag gcacattacc
     5401 ttgaatcgtg gagcgacgtg cgcgcgtacg atgggaccgt gacgatcatg cagcccctga
     5461 ttgcgccatt gtatggtgga cgcacggcgc acgagattct tgcagccgtg gccggtcagg
     5521 cggagcgctc gagctatgaa atcgtccacg attactggcg cgctcaaagc aagacgaaga
     5581 atttcgatgc ggcgtggcgc accgcgttgc acgatgggtt cattgcagga agcgcctttt
     5641 ctccgcgcaa cgtaagacca aagccggcgg cgagctttga tacttccgaa ccacagcgcc
     5701 cgagcaccgc cgccgcggct aaaacgccaa atgccccatc gctggaagtt atctatcggc
     5761 cagatccttc gattatcgac ggccgctttg cgaacaacgg gtggcttcaa gagctgccca
     5821 aaccgctaac caaattgacc tgggacaacg ccgttctgat gagctccgcg acggcgggga
     5881 aactcggtct cacctaccag gtgggaagca caggcggtga acacggaacc atatatgccg
     5941 atgtcgtgga attggaatac gacggccgac gaacccgcgg ccccgcctgg attgtcccgg
     6001 gccatgccga tgattgcgtt acgattcacc tgggctatgg ccgcagacgc gcaggacgga
     6061 tcggcacgaa tgtcgggttc aacgctaacg cgctccgaac gctagagaca ctctggcaca
     6121 acgcgtccat cgccattcgc aaaaccgatg gtcaatatgc tctcgcctgc acgcagtttc
     6181 atcacctgat ggaagggcgg gatctggtga gagcgggaac gatcgaacaa taccggaaaa
     6241 atcccaagtt tctgatcgaa cgggagagcg aaaaggaagc acccgccact ctttaccccg
     6301 gctacaaata cgacggctat gcctggggca tgagcatcga cgtcaatgca tgcatcggct
     6361 gcaacgcctg tgtggcggcg tgccacgcgg aaaataacgg acccgtggtg ggaaagcagg
     6421 aagtgttgcg cggccgcgaa atgcattgga tccgggttga tcgctatcat cgaggagaac
     6481 cgagcaatcc cgagacttac tttcagcccg tgccctgcat gcactgcgag aacgcgccct
     6541 gcgagctggt ttgtccggtg ggcgccacga cccacagtca ggacggactg aatgacatgg
     6601 tttacaaccg ttgtgtcggc acgcggtact gttcgaataa ctgtccctac aaagtgcggc
     6661 gctttaattt tttcctgtac agcgatttca aaaccgagag cctgaagccg ttacgcaatc
     6721 cggacgtgac ggtgcgttcg cggggcgtca tggaaaaatg cacctactgc gtgcagcgca
     6781 tcaaccaggc gaaaatcgcg gcggaaaaag aggagcgcac ggtgcgcgac ggcgagattg
     6841 agcccgcatg ccagcaggcc tgtccgacac gggcaatcat ttttggcaac atcaacgacc
     6901 cgcagagccg tgtcagccaa ctgaaggcac aaccgctgag ctatgggtta ctgaccgagt
     6961 tgaacacgcg gccccgtacc acgtatcagg cgaagctcag gaatccgaac ccggaaatcg
     7021 agaacggctg agatggcatc gaaaccaagc gacgtttccg gcgactctct gccggttctc
     7081 gagcccggtc acaccttcgc ctcgattacc gacaagatca gctcgattgt cttgaccggt
     7141 cgcacaccgc tgttttggta catcagcttt ggtatcggtt ttcttttggt tctgctgcta
     7201 cttttttgca tcacggcgct cgtctccgtc ggcatcgggc ttttcggcgt gatgattccc
     7261 gtcgcgtggg gctgggcgat tgtgaacttc gtctggtgga tcggcatcgg ccacgccggc
     7321 acgttaattt ctgcgatcct tttattgctg cgccagaaat ggcggcagtc gatcaaccga
     7381 ttcgccgaag cgatgacatt gttcgccgtc gcctgcgcgg gaatttttcc cctgattcat
     7441 ttgggacggc catggtttgc ttactggttg tttccttacc cgagcaccat gggcatccag
     7501 ccgcagtttc gtagtccact agtttgggat gtgttcgccg tctcaaccta tttcacggtt
     7561 tctttgctgt tttggtttct tggcttgatt ccggatctgg caaccctgcg cgatcgttcc
     7621 acccgtcgcg tgccgcgatt catttatgga atgctcgcga tggggtggcg cggatcggcg
     7681 gtccattggc accgctacga gagcgcctat ttgttgctcg cgggattggc tactccgttg
     7741 gtggtctcgg ttcacaccgt ggtgagcttt gatttcgcgg tggctctcgt gccgggatgg
     7801 cattcgacca ttttcccacc gtatttcgtt gcgggggcaa tctattcggg ttttgcaatg
     7861 gtgctgacaa tcgccatacc gctgcgcaag ctctatggac tcgaggattt catcacgctg
     7921 cgccatctga agaacatggg gatcgttatg ctcgccaccg gcatgatcgt cgcctacggc
     7981 tacctgatgg aaactttcat ggcctggtac agcggcgatc ttttcgaaaa atatatgatg
     8041 atgaaccgca tgttcggtcc ttacgggtgg ctctattggc tcttgatcct gttcaatatt
     8101 ctcattccac aggcgctttg ggtgaggagc ctgcagacca atccgtgggt gctgttcttc
     8161 attgccatcg cggtgaacat cggcatgtgg ctggagcgct acgttatcgt ggtagtgagc
     8221 ttgcaccgtg atttcatgcc ttcggcttgg ggaatgtact cgggcacgat cttcgattac
     8281 ggcgtgttcg tcggcaccat cgggttgttc gttgccttac tgtttctgtt catccgttta
     8341 ctgccgatga tttcgatctt cgaaatgcgc gagttggttc acgaagaaga ggggcgcggt
     8401 gaatgaaaca ttcgtgacaa tttcagccgc gcatgaggtc tcggttattc gtggctaggc
     8461 tacctatata tggcctgatg gctgagttcg acagcccgga ggcgctgctc gaggcgagcc
     8521 ggcgggcttt tgccgaaggg tttcgcagaa tggatgccta ttcgccattc ccggtcgacg
     8581 gactggctga agcgatcggc tttcctcgca cgcgggtgcc gctgattgta ctgattggcg
     8641 ggattgtcgg ctgcctcggc ggattttggc tgcagtattg ggtttccgtg atcgactatc
     8701 cgatcaatat aggcgggcgc ccactcaaca gctggccgtc ctttataccg gtcaccttcg
     8761 agttgaccat tctgctcgcc gctttggcgg ctttctttgg cgtattggga ctgaatcgcc
     8821 tgcccatgcc ttaccacccg gtatttaacg tcgaccgctt cgaactcgcg agccgcaacc
     8881 gtttttttct ctgcatcgaa gcggcggaca acaaattcga gcttgaccgc acgcggcgct
     8941 tcctcgatga gatcggctcg ttgggaacgt atgaagtgga accctaagca catgtggcca
     9001 attcaccgcg caaaaaaaac actctgctgt ttgatcctga caagtggcct ctggttcatg
     9061 gcttgccagc agaaaatggc ggatcagccg cgttacgagc cgttgcagaa gagtgatctt
     9121 ttcgacgatc agcgcgcgtc gcggccgctc gtcgaaggca cagtgccacg cggtcagctg
     9181 aatgcagatg cggaattcta taccgggaaa gtgaacgacg aaccggctaa gacatttcca
     9241 ttcaccgtcg atcgggagct gctcctccgc ggtcaggaac ggtttacgat tttttgttcg
     9301 ccttgccaca gtcgattggg cgacgggcag gggatgattg tccgacgtgg atttcggccg
     9361 ccgccctcgt ttcatatcga gcgcttgcgc gaagctccgc ccgggcactt ttttgatgtc
     9421 atatctcacg gctttggcgc gatgccggat tacgccgaac aaatcccacc gcacgatcgc
     9481 tgggcgatcg ccgcttatat tcgcgctctg caattgagcc agagcgccgc gctcacggat
     9541 gtaccggaaa cggagcggcg ggcgcttctc gaggaaaaaa aatgaacggg ctcgacgtga
     9601 cccttagcac cgaactcgat cccttgcgga cgaaatcctt gatcgcgggc ggtttggcgc
     9661 tcgtgctctg ttttatagga tggtttttta atcgcgggga attttttcgc gcctatctca
     9721 tcgcatacgt tttctggatc ggtattccgc tcggttgcct cggtattttg atgatccatc
     9781 atctggttgg tggaacgtgg ggttttgtca tccagcgctg cttggaagcc gcggtgcgca
     9841 ccttcccgat catgttcctg ctgttcctgc cgctgttgct cggcttgccg gatctttttg
     9901 tttgggcacg gcgcgacgtc atatcggccg accctgtgct tcaggaaaag gcagcgtacc
     9961 ttaatatgcc gttctttttc gttcgggcgg tcgtgtattt tgccgtctgg tttgccatcg
    10021 gttttttcct gaccaagtgg tcgcaagcgc aagagcgctc ggcggatcca gcgttcacgc
    10081 aacgcctcca gaccctgagc ggtcccggtt tggtcctcta tggtttaacg gtgacttttt
    10141 ccgcgatcga ttggctgatg tcgctggaac cgcactggta ctcgaccatt ttcggtatga
    10201 tctttatggt gagccacggc ttgatcgcat tgacgtttgt catcggcgct gcgtattttt
    10261 tgtcccgccg cgaagcactc cggcagatcg tcgctccctg ggtgctgcag gatctgggca
    10321 atttgctcct ggcttttgtg atgctctggg cttatctgtc tttttcccag ttcctcctca
    10381 tctgggttga aaacctgcaa cacgagatcc cctggtactt acacaggttg accggcggct
    10441 gggctgtgat cggcatcgca ttgatcgtgg tgcaatttgc cttgcctttt gtgctgttgc
    10501 tgtcgcgcgc cgtcaagcgc aacccggcgg ctctcgcagc cgtggccgga ggggtcgccg
    10561 ccatgcattt tatcgagctg ttctggtttg tggcgccgac gtttcacccg gacggtttta
    10621 gcctgcactg gacgacgatc atggcgccgg taggtatcgg cg
//
LOCUS       DASULZ010000855         4672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_708085, whole genome shotgun sequence.
ACCESSION   DASULZ010000855 DASULZ010000000
VERSION     DASULZ010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708085"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1074)
                     /locus_tag="VFY96_17565"
     CDS             complement(91..1074)
                     /locus_tag="VFY96_17565"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5608339.1"
                     /translation="MTNSRAKSLTLLLIAIVGFTWTSAAEAQKKVRFAYPSSADMGDI
                     PSLLAWEQLKKQGIEVVPTFFPKTELAVQAVAAGEADIGSAAGIAVVKAVESGMNVRI
                     IGEQVRNEWQLVTPVSFKDPKQLDGKRVGYHAPITVTEALVKWMAQHYKISPNWMIIP
                     GSEVRAEALMRGQLDATPAEIGDVLNILSAKPGQFHVLISYAKTFPQLIGSMYFARGD
                     YVQKNASTVESVLEAILRAHRDAEARPASVKENALRLMPETKPELVDAIANTYKELRI
                     WDVNGGADRQRGEASIKFFEESGLLKKDAVSFNQAFDTGPLDKVLKKIGRK"
     gene            complement(1106..1861)
                     /locus_tag="VFY96_17570"
     CDS             complement(1106..1861)
                     /locus_tag="VFY96_17570"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5608340.1"
                     /translation="MNTRFGSNVLSMLSLAILWEVAGRAMNSSLIPPLSQIATAWWKL
                     LSSGKLLANLSMSLTTLAIGFLLAVFVGVVLGLLMGRFRAVEHFLDLYVNALMSAPTT
                     AFVPVLILWFGLGMESRIAVVFLFAVFVIIINTMTGVKQVDSVLVEMARSFGAKEKEI
                     FFKIILPAALPAIMAGLRLGIGRAVKGMVTAEMLLTLTGIGAMIMQYGSAFATDSLFA
                     VILTVLLVALVTMKAVQSLDRRLTGWKAEIAVE"
     gene            complement(1858..2745)
                     /locus_tag="VFY96_17575"
     CDS             complement(1858..2745)
                     /locus_tag="VFY96_17575"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5608341.1"
                     /translation="MSVQDVQAVQAVQTVRESLGSSNSLNGLNGLNASKSLVDIQGVS
                     KSFHKTIKDNTTEIKALSDVSLSIRENEFVSIIGPSGCGKTTLLKMIDGLIPYDSGKI
                     VINGKPVTAPGPDRAVVFQSFALLPWRTVLANVEFSLELRQLSKEQRAKTARDYLKRV
                     GLADFENHYPHELSGGMQQRAGLARALAVNPAILLMDEPFGAVDAQTRQLLQEELLEL
                     WQRERKTVIFVTHSMDEAVYLSDRVVVMTPRPGKVAEILDVPLPRPRVSEQVRRHVKF
                     VDLTNYIWESLKRAMENHH"
     gene            complement(2747..4180)
                     /locus_tag="VFY96_17580"
     CDS             complement(2747..4180)
                     /locus_tag="VFY96_17580"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX5608342.1"
                     /translation="MAKDLRSYLDELLEKRPKDVVVVDREVDPRYEASAIVEKFERKN
                     EYPLVFFKNIKGSKIPLVINLGATYERLALSLGSPSVPQMVKDLAFREHHHMPVKEIP
                     AKDAPCKEVILKGDDADLDLLPVLTLNEGDAGSYINAAALICKERGTNAVNVGIYRHQ
                     KQGKRQLGLMINPANHGNYIRAEYEDHNEPMEVALAIGHHPALNMAAVSKQAGVGSEM
                     EVAGAFLGEPLEVVKAETVSLMVPARCEIVIEGVVPPHKRHYEGPFGEWPHYYYKEGD
                     QPYIEVTAITMRKNPIYQCIHSAHFEHNIIGAAPRLGSLLKRIQEVVPSTTGVNMPIS
                     GAARSHCYISVKKRAEGDPKQAALAAIVTDPNIKHVIVVDDDVDVFNETQVLWALAMR
                     FQADRDLVIIPNIIGSHLNPTAYGYNRLEKGPMETKLIFDATKPLPPYHFPKEAQAPD
                     EVLNKIDLARDTRPYQPGKDDEAITGK"
     gene            4315..>4672
                     /locus_tag="VFY96_17585"
     CDS             4315..>4672
                     /locus_tag="VFY96_17585"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5608343.1"
                     /translation="MARNLPVRWGLSLANRGALFGLTDVDELIESAIFAEQSAVFDSV
                     WVGDSLIHKPRLEAITLLSALATRTEKLRLGTICMASFPVRHPVLLAIQWASLDQISK
                     GRTILGVCIGGGHEGEL"
BASE COUNT          978 a         1314 c         1295 g         1085 t
ORIGIN      
        1 gcgttgccgt ggcggccggc gaattcccag attgccgcga gtgtgagcag cgaaatgact
       61 ctaagcgtcc ccggtttcat tactctagcg ctatttgcgt ccgatctttt tgagtacttt
      121 gtccagcgga cccgtatcaa atgcctggtt aaagcttacg gcgtctttct tgagcaggcc
      181 ggattcctca aagaatttaa tgctcgcctc gcctcgttgt cggtcggcgc cgccattaac
      241 gtcccaaatt cggagctcct tgtaagtgtt agcgatcgca tccactagtt ccggcttggt
      301 ctcaggcatg agtcgcaggg cattttcctt gaccgatgcc gggcgagctt cggcatcgcg
      361 atgggctctg agaatcgcct cgagcacgct ttcaacggtc gaggcattct tttgcacgta
      421 atccccgcgc gcaaagtaca tcgacccaat gagctgcgga aaagtcttgg cataggagat
      481 caaaacatga aactgcccgg gcttggcgct tagaatattc agcacatcgc cgatctctgc
      541 cggagttgca tccagctggc cgcgcatcag cgcttcggcg cgaacctccg agcccggtat
      601 gatcatccaa ttgggcgaaa tcttataatg ttgcgccatc catttgacca gcgcttcggt
      661 gaccgtaatc ggcgcatgat agccgacccg tttgccgtcc aactgtttcg gatctttgaa
      721 cgataccgga gtcacaagct gccactcgtt acgcacctgt tcgccgatga tgcgaacatt
      781 catgccgctt tcgacggctt tgaccacagc gatgccggcg gcagaaccga tgtccgcctc
      841 tccggcggcg actgcctgca cggccaattc ggttttcggg aagaaggtcg gcacgacttc
      901 gatcccctgt tttttgagct gctcccaagc gagcagcgaa gggatatcgc ccatgtccgc
      961 cgatgatgga taggcgaagc gcactttctt ttgcgcctcg gcagcgctgg tccaggtgaa
     1021 acccacgatg gcgatcaaga gcagtgtcag cgacttcgcg cgtgaattag tcatggccgt
     1081 ttctcctcct gctatcttac gcccgctatt cgacagcgat ctctgccttc catccggtta
     1141 gtctgcggtc gagcgattgg accgctttca tcgtcactaa cgccacgagc aagaccgtaa
     1201 gaatcacggc gaagagcgag tctgtggcga atgctgagcc gtattgcatg atcatggcgc
     1261 cgatgccggt gagcgtaagc agcatttccg ccgtcaccat gcctttgaca gcccggccga
     1321 taccgaggcg cagaccggcc atgatcgccg gcagggctgc gggcagaata atcttgaaga
     1381 aaatttcttt ttctttcgcg ccgaaggaac gcgccatttc aaccagaacg ctgtcgacct
     1441 gcttcacgcc ggtcattgtg ttgatgatga tgacgaatac ggcaaaaagg aaaactacgg
     1501 caatacgcga ctccataccg agcccgaacc agaggatcag taccggcaca aacgccgtgg
     1561 tcggtgccga catgagcgca ttcacataca agtccagaaa atgctcgacc gcgcgaaaac
     1621 gtcccatcaa aagaccgaga acaacaccga caaaaaccgc cagcagaaaa ccgatggcaa
     1681 gtgtcgttaa actcatgctc aagttagcaa gcaatttgcc gctgctcaaa agcttccacc
     1741 aggctgtggc gatttgggac agtgggggaa tcaagctgga attcatggcg cgcccggcga
     1801 cctcccaaag aatcgccagc gaaagcatcg agaggacatt gctgccgaag cgggtattca
     1861 atggtggttc tccatcgcgc gcttgagact ttcccagatg taattagtca agtcgacgaa
     1921 tttaacatgg cggcggacct gttccgagac gcgcggccgc ggcagcggca cgtcgagaat
     1981 ctccgccact tttcccggcc gtggagtcat gacgaccaca cgatccgaga gatacactgc
     2041 ttcgtccatg ctgtgggtaa caaagatcac cgtcttgcgt tcacgttgcc acagctccag
     2101 gagctcttcc tgtagcagtt gacgggtttg cgcgtccacc gcgccgaagg gttcgtccat
     2161 gagtaagatc gcaggattga cggcgagtgc ccgcgccaac ccggcgcgtt gctgcatgcc
     2221 gccggagagc tcgtgcggat agtgattttc gaagtcggcc agaccgacgc gcttcaaata
     2281 atcgcgtgcg gtttttgccc gctgttcttt ggacagttgg cgcagctcca agctgaactc
     2341 gacattcgcg agcacagtgc gccaaggcaa caaggcaaac gattgaaata cgactgcacg
     2401 gtcgggaccc ggcgcggtaa ccggcttgcc attgattacg atcttgccgc tatcgtacgg
     2461 aatcaagccg tcgatcattt tcagcaaggt agttttgccg caaccgctcg ggccgatgat
     2521 gctgacaaac tcgttctcgc gaatcgaaag actcacatcc gacaaggcct tgatctccgt
     2581 cgtgttgtct ttgatggttt tatgaaagct cttcgagacc ccctggatgt caaccagaga
     2641 cttcgacgcg ttcaaaccgt tcaatccgtt caaactgttt gaagagccga gcgattctcg
     2701 gacggtttga accgcttgaa ctgcttgaac atcttgaacg gacaattcat tttccggtga
     2761 ttgcctcatc gtccttgccc ggctggtaag gccgtgtatc gcgcgcgaga tctattttgt
     2821 tcagcacctc atccggcgcc tgcgcttctt taggaaagtg atagggcggc aacggtttgg
     2881 tcgcgtcgaa aattaacttc gtttccatcg gccctttctc gagacgattg tagccatagg
     2941 cagtgggatt caaatgggag ccgataatgt tggggataat caccagatcg cgatcggctt
     3001 gaaagcgcat cgccagcgcc cagagcactt gcgtttcatt gaagacatcg acgtcgtcgt
     3061 cgaccacaat cacatgtttg atattcgggt cggtaacgat ggccgcgagc gccgcctgct
     3121 tgggatctcc ctcggcgcgc tttttgaccg agatgtaaca atgggagcgg gcagcaccgg
     3181 agatcggcat gttgacgccg gtcgtgctgg gcaccacttc ctgaatgcgc ttcaaaaggc
     3241 tgcccaaccg gggcgccgcg ccgatgatgt tgtgctcgaa atgagcgctg tggatgcact
     3301 ggtagatcgg gtttttgcgc atggtgatag cggtgacttc gatgtagggt tgatcgcctt
     3361 ccttataata gtagtgcggc cattcgccga acgggccttc gtagtggcgt ttatgcggcg
     3421 gtacgactcc ctcgataacg atctcgcaac gtgccggcac catgagactg acggtttccg
     3481 ctttgaccac ttccaaaggc tcgcccaaga acgcaccggc gacttccatc tccgatccga
     3541 caccggcttg tttgctgacc gcagccatat tcaacgctgg atgatggccg atggcgaggg
     3601 cgacttccat cggctcgttg tgatcctcgt attctgcccg gatgtaatta ccgtgattgg
     3661 cgggattgat catcaagccg agctgtcgtt taccttgctt ctgatggcgg tagataccaa
     3721 cattaacggc gttggtgccg cgttctttac agatcagagc ggcggcattg atgtaggaac
     3781 cggcatcgcc ctcgttcaat gtaaggacgg gcaggagatc gagatcggca tcgtcgcctt
     3841 tcaagatcac ctctttgcac ggggcatctt tggcaggaat ctctttcacc ggcatgtgat
     3901 gatgttcgcg gaatgccaag tctttgacca tctgcggaac cgagggcgac cctaatgaca
     3961 gcgctagacg ctcgtaggtt gcgccaagat tgatgaccag cggaattttg gagcctttga
     4021 tgtttttgaa gaacaccagc gggtattcat ttttgcgttc gaacttttcg acaatcgcgc
     4081 tggcctcgta gcgcgggtcg acttcccgat cgacaacgac gacgtccttc ggccgtttct
     4141 cgagcagttc atccagatag ctgcgcagat ctttcgccat attcgcctcc ttgcatgctt
     4201 ggggcagtcc cggtctgctc cgcgcgtgag ttctaaatac acccagtcca atcagtctgg
     4261 caacccgcag ctttgcttga cagaccgtac cccttcttct aactgtgggc ggtcatggcg
     4321 cgaaacctac cggtgagatg gggcttgagc ctcgccaatc gcggcgcgct cttcgggctg
     4381 accgatgttg acgaattaat cgaaagcgcc atttttgcag agcagtcggc tgtattcgac
     4441 tccgtttggg ttggcgatag cctaattcac aagccgcggc tggaagcgat tacgctgctt
     4501 tcagcgctgg caactcgtac cgagaagctt cggcttggga ccatctgcat ggcttcgttt
     4561 ccggtgcgcc atccggtttt gctcgcgatc cagtgggctt ccctcgacca gatatcgaaa
     4621 gggcggacga ttttaggcgt ctgtatcggt gggggccatg agggcgagct cc
//
LOCUS       DASULZ010000856         3149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1317258, whole genome shotgun sequence.
ACCESSION   DASULZ010000856 DASULZ010000000
VERSION     DASULZ010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317258"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="VFY96_17590"
     CDS             <1..800
                     /locus_tag="VFY96_17590"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5608344.1"
                     /translation="IELEPLLISGGALNMQALLAGSVQMSQNSASSAIQAALRGAPVV
                     LAAALENRMPLQIIARPEIKTPQQLIGKKIGILRYGGSNDTGVQWALRRWKIEPRQVA
                     ILQSGATNARMTALTLGQIDATILSYPEIHLARKLGMNVLADIGDFNAYPNTSLLFTR
                     SFIEKQRPLVKNLLRSQVEAIHYVRTNRDGSIKVLKKYLRVDDAEAIAATYDFFAPRT
                     ELPPRPNLDGIKSILTEMGAPQRAPEEFVDLSLLDEIEREGFLQRLR"
     gene            894..1367
                     /locus_tag="VFY96_17595"
     CDS             894..1367
                     /locus_tag="VFY96_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013172856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX5608345.1"
                     /translation="MDAIWIKRAHRGPMDPVSSAFLSARRGLVGNADQGGSRQVTLIE
                     REVWQEVMIAMHGSLQPAVRRANLLISGVPLAGSRGQVLRIGEARLKIVGETKPCERM
                     DEALPGLQVALYPEWRGGAFASVLSDGEIKTGDAVLWEEQAQASMTFMHENEKRN"
     gene            1372..1734
                     /locus_tag="VFY96_17600"
     CDS             1372..1734
                     /locus_tag="VFY96_17600"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX5608346.1"
                     /translation="MDDVEGSGEPAKKGDRVIYNVRIHLNKGEEVPLNERQAENIPPN
                     MLRTENGRRLVDHELILGKRRAIAGIEYALIGMRPGGYRKVRLSPHLAYRDEGLADLI
                     PPNAVLNVELWLREVITM"
     gene            1848..2840
                     /locus_tag="VFY96_17605"
     CDS             1848..2840
                     /locus_tag="VFY96_17605"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX5608347.1"
                     /translation="MADIFISYASKDRARAGMLAQALGARGWSIWWDREIPLGRSFDE
                     VIEKALADAQCVVVLWSAVSIASEWVRNEASEAKRRGILIPVFIEPAEAPLAFRLLNG
                     ADLRDWRGDPEDGEFQRLVERVEELLAERTAPGAGRQIRVESPPVYRERQKRSPRTML
                     LAVGAIGIVGAAIAGYLWRGDQSEPRRPDPAISIPRTAEPELGSTDMEKRIRDLVGSL
                     GTAVPATSLAKGFHVPDLGLRIAYLTPEQSASTLGAMPAGAVVMEVESGRPIAKAGIH
                     VGDVVLSIGSRKIASEDDLRQAIFKIGPGKTEYSYRRGSDTHRIAVDCPACKAE"
     gene            2868..>3149
                     /locus_tag="VFY96_17610"
     CDS             2868..>3149
                     /locus_tag="VFY96_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003270148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEX5608348.1"
                     /translation="MKSGKQRRKEILRKRRTRPSNAGRDDTRVIDPRPDNLVPVNAEL
                     LAPNNSYGAPDFVTRGYYVDRNFCCIDCGKEEVWTGTQQKWWYEVAKGFA"
BASE COUNT          705 a          837 c          945 g          662 t
ORIGIN      
        1 aaatcgagct cgagccgctg ttgatcagcg ggggcgcgct aaatatgcaa gcactcctcg
       61 ccggaagcgt tcagatgagc cagaactcgg catcgtcggc catccaggcg gcgttgcgcg
      121 gcgcaccggt ggttctggct gcggcgctgg aaaaccgcat gcccttgcag atcattgccc
      181 gtcctgaaat taaaacgccg caacaactga tcggaaaaaa gatcggcatc cttcgttatg
      241 gcggctcgaa cgacactggg gttcaatggg cgctacggag gtggaaaatc gagccgcgcc
      301 aggtcgcgat tctgcagtcg ggcgccacca atgcgcgtat gactgcgtta accttaggcc
      361 aaatcgatgc gacgattttg tcctatccgg aaattcacct tgcgcgcaag ctcggtatga
      421 acgtcctcgc ggacatcggt gactttaacg cgtatccgaa tacctcttta ttgttcactc
      481 gctccttcat cgaaaaacaa aggcccttgg taaaaaacct gctccgttcg caagtcgaag
      541 cgattcatta tgtcaggacc aatcgcgatg gaagcatcaa ggttctaaaa aaatatttgc
      601 gcgttgacga cgcggaggcg atcgccgcga cttatgattt cttcgcgccg cgaaccgagc
      661 tgccgccgcg ccccaacttg gacgggatca aatcgattct gacggaaatg ggcgccccgc
      721 aacgcgctcc cgaagagttc gtcgatttga gtttgctcga tgagatcgaa agggaaggct
      781 tcctgcagag attacgttag tcaaattgac tattcgcgat cactttaact tatcggaagt
      841 aatactttat cttgggtttt ttccttatga ggtcagcgac gagtgtcggt cgattggatg
      901 cgatatggat caagcgtgct catcgcggac cgatggatcc cgtgtcttcg gcttttttat
      961 cagcgcgccg gggactggtg ggtaatgccg atcaaggcgg atcacgccag gtaacgctca
     1021 tcgagaggga ggtttggcag gaggtgatga tcgcaatgca tggttcacta caacctgccg
     1081 tccggcgcgc caatttgctg ataagcggcg tgccgctcgc gggctcgcgc ggacaggttt
     1141 tgcgcatcgg cgaagctcgg ctgaaaattg tcggcgagac gaaaccgtgc gaacggatgg
     1201 acgaggcttt gccagggcta caagtggcgc tctatcccga atggcgcggc ggcgcattcg
     1261 ctagcgttct aagcgacggc gagatcaaaa ccggtgatgc tgtgctgtgg gaagagcagg
     1321 cgcaggcctc gatgactttc atgcatgaaa acgaaaagcg gaattagact tttggacgat
     1381 gttgaaggct ccggcgagcc ggctaagaag ggcgacagag tcatttacaa cgttcgcatc
     1441 catctcaaca aaggcgagga agtgccgctc aacgaacgcc aggccgagaa tataccgcca
     1501 aacatgctgc gcactgaaaa tggccgtcgt ttggtcgatc acgaattgat cctaggcaaa
     1561 agaagagcaa tcgccgggat cgaatatgcg ctgatcggca tgagaccagg cggctatcgt
     1621 aaggtgcgcc tcagccctca tcttgcttat cgcgacgaag gcctcgccga tctgatcccg
     1681 ccgaatgcgg tattgaatgt tgagctctgg ttgcgcgaag ttatcacgat gtaaatcgtt
     1741 cagcggcgac cgagcgatct ccgctcaatc actgattcgt cgtcgttcga tttcggctgg
     1801 tctgttcgcc ggcagtctta taaaatagcg cgatctatct taattccatg gccgatattt
     1861 tcatcagcta cgcttcgaaa gaccgtgctc gcgctggaat gctggcgcaa gcactcggtg
     1921 cgcgcggctg gtcgatttgg tgggaccgcg aaattcctct cggcaggtcc ttcgatgaag
     1981 tcatcgaaaa agcgctggcg gatgcccaat gcgtcgtcgt cttgtggtcg gcggtttcga
     2041 tcgcttccga atgggtccgt aacgaagctt cggaggcgaa gcggcgcggg atcttgattc
     2101 cagtatttat cgaaccggca gaggcgccgc tagcctttcg acttttgaac ggagccgatt
     2161 tgcgggattg gcgcggtgat ccggaggacg gcgaattcca gcgcctggtc gaacgagtag
     2221 aggaattgct cgcagagaga acggcgccgg gtgcgggacg acaaatccgg gtggagtccc
     2281 caccggttta ccgcgagcgt cagaagcgct caccacggac aatgttgctc gcggtcggcg
     2341 cgattgggat tgtcggcgcc gcaatcgccg gttatttgtg gcgaggagac caatccgaac
     2401 ctcgccgtcc agatccagcg atttcaatcc ccagaacggc ggaacccgaa ctgggcagca
     2461 ccgatatgga aaagcgaatc cgcgatctcg tgggctccct cggcacggcc gtgccggcca
     2521 cttctctggc taaaggattt catgtgccgg acctcggctt gcgcatcgct tacctcaccc
     2581 cggagcaaag cgcatcgacg ctcggggcca tgcctgcggg agcggtagtg atggaagtcg
     2641 aaagcggtcg gccgatcgcc aaagcgggga ttcacgtcgg cgacgtggtg ctctcgatcg
     2701 gcagcagaaa aatcgctagc gaagacgatc tccgccaggc gatctttaaa atcggtcccg
     2761 gcaagactga atactcgtat cgacgcggga gcgataccca taggattgcc gtcgattgcc
     2821 cagcttgcaa agcggagtga ccgctgctcg gttattaaaa tttttcgatg aaaagcggca
     2881 aacagcggcg caaggagatt ctccgtaagc gccgaactcg gccgtcaaac gctgggcgcg
     2941 atgacacgcg ggtgattgac ccgaggccag acaatttggt cccggtcaac gcggaactgc
     3001 tcgcgcctaa taacagctat ggcgctcccg atttcgttac aaggggctac tacgttgacc
     3061 gaaacttttg ctgtattgat tgtggcaaag aagaggtctg gacgggaacc cagcagaagt
     3121 ggtggtatga agtcgcgaaa gggttcgcc
//
LOCUS       DASULZ010000857         7059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1419748, whole genome shotgun sequence.
ACCESSION   DASULZ010000857 DASULZ010000000
VERSION     DASULZ010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 7059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7059
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419748"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1743
                     /gene="lon"
                     /locus_tag="VFY96_17615"
     CDS             <1..1743
                     /gene="lon"
                     /locus_tag="VFY96_17615"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX5608349.1"
                     /translation="QMRAIQKELGEKDEFKNEIQEIEEKLKQKKLSAEAKDKVEKELK
                     KLKMMSPMSAEATVVRNYIDWIISLPWNDFTDDKLDINEAERVLEDDHYGLEKVKERI
                     LEYLAVQSLVGKIKGPILCLVGPPGVGKTSLGRSIARATGRKFVRVSLGGVRDEAEIR
                     GHRRTYIGALPGKIIQSMKKAGSSNPVFLMDEIDKMSTDFRGDPSSALLEVLDPEQNT
                     AFNDHYLDLDYDLSKVMFITTANTLDRIPRPLQDRMEIIRIAGYTELEKLSIAKKYLL
                     DKQKEANGLTADNLVFTDNAILGVVRHYTKEAGVRSLEREIAAICRKVAVEVVRRDRN
                     AHIQIGSKSLHKYLGPVKFRYGKAETEVKIGVTTGLAWTELGGELLATEVTIMPGKGQ
                     LIITGKLGDVMQESAQAAMSYVRSRATELGLAKDFYQKLDVHIHVPEGAIPKDGPSAG
                     ITMATSLVSALMKLPVHNDLAMTGEITLRGTVLPIGGLKEKILAAHRAGIKKVLIPAE
                     NEKDIEEIPATVLKTVELELVSHMDQVLKKALVLDDPESLFRREPLENQAKDEPPPFA
                     EKVDDVPASEILPQ"
     gene            1973..2047
                     /locus_tag="VFY96_17620"
     tRNA            1973..2047
                     /locus_tag="VFY96_17620"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2005..2007,aa:Val,seq:tac)
     gene            2073..2149
                     /locus_tag="VFY96_17625"
     tRNA            2073..2149
                     /locus_tag="VFY96_17625"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2107..2109,aa:Asp,seq:gtc)
     gene            2197..2622
                     /gene="rplM"
                     /locus_tag="VFY96_17630"
     CDS             2197..2622
                     /gene="rplM"
                     /locus_tag="VFY96_17630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX5608350.1"
                     /translation="MKKQEALSQRRWYVIDGQGKILGRMATEIARVLRGKHKPTFTPN
                     QDTGDFVIVINARGVKLTGAKLQDKIYYRHTEFPGGIRERTAAEMLQIKPEELVKLAV
                     RGMLPKNRLSRKLVTKLKVYAGPEHPHTAQKPEPLTIAS"
     gene            2632..3024
                     /gene="rpsI"
                     /locus_tag="VFY96_17635"
     CDS             2632..3024
                     /gene="rpsI"
                     /locus_tag="VFY96_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX5608351.1"
                     /translation="MAEKVFTATGRRKTSVARVFLKPGKGRITINAKSLQEYFGRETA
                     RMIVLQPFDVTQTSGNFDIDVNVVGGGNSGQAGAIRHGITRALMQINPELRSPLKKAG
                     FVTRDPRAVERKKYGRHKARKRPQYSKR"
     gene            complement(3163..4269)
                     /gene="lptG"
                     /locus_tag="VFY96_17640"
     CDS             complement(3163..4269)
                     /gene="lptG"
                     /locus_tag="VFY96_17640"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEX5608352.1"
                     /translation="MGRLAFKFLPVGAILDRYVASNFLRIFFISLLVITLVYTTVDFF
                     ERIGALLDAGAPLFTILRYFFYKAPLLISRVIGFAVLFSTLFCLGALTRTHEITAMRA
                     SGITVQRIALPLLLLSLIVCALNFLWNEALVPVFAHNAQTIYKTEIKNKQQQSLLGTH
                     DIWIRAENSFINIDNFDPRTNVLENVTVFLLNRDFSLRGLLEIPRAQYTQNGWNATGA
                     TEWTLLPDGKMVQRETTTTLPLSETPKDLQLLARDAEEFTFFDLQKQIADMKAKGVDP
                     TYYEVDLQTKLALPFISPLMVLLAIPFAIKRKMTGTLSLSFGIAMVIAFGYWVLAAFC
                     LSLGHSGAMPSLISAWAPNAIFAMIGLFFFTAEE"
     gene            complement(4269..5549)
                     /gene="lptF"
                     /locus_tag="VFY96_17645"
     CDS             complement(4269..5549)
                     /gene="lptF"
                     /locus_tag="VFY96_17645"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEX5608353.1"
                     /translation="MASYLAISRSRILQTVSHSLTCLDVKRRILFRYLISEIIPPFLL
                     GLLAFTSVLLIGRILRLIELVVTRGVPILQILKLFSLILPTFLEMTVPMAFLLAILLG
                     LGRMSNDQELLAMKASGISPAQILWPVVALASVIALITFFLTLFARPVANFALKKELY
                     NIAKNRVGTALKEKIFNDDFPKILIYVEEIIPPGNTAQGVLIVDKRDESREDIILGKV
                     ARISTEEETNTLGLRLFDGSIYEREKNRPGFSQTRFNIYDFKLDLDDLIGPIQQKESG
                     PKEMTVSELLSSIEKKQRAGAKTIAERMELHQRISFGFVPLLFCLLGVALTLVPRSSR
                     ANRSWGFMLCLFWLVAYYALLSLGKALGDKNILNPIPALWLPNLVVGAIGAHFFRHAL
                     RDSPLSFPRVLDRAAESIARDWVELRRSLHRKHN"
     gene            5548..6309
                     /gene="rlmB"
                     /locus_tag="VFY96_17650"
     CDS             5548..6309
                     /gene="rlmB"
                     /locus_tag="VFY96_17650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX5608354.1"
                     /translation="MSSRTEIIFGINPVQEKVRASPKDVLEILFAEAGDRAALRSIER
                     EAVRHGVRVSRVPVHLLDRLASGQRHQGVIAKIECFRYLSLDQFLERISAAVNSEWIL
                     ILDGVTDPRNFGALLRTAEAVGIGHVVIPKHRSAEVSPLVVKTSSGATFYLNIVKATN
                     LRRALWELKKRGYWIVGLDAKATKTIYENNYPERLVIVLGSEGKGIRQINLRECDFVV
                     SIPMLGKVASLNVAVAGAVFLYEVLRQRQAGVVEH"
     gene            6531..6605
                     /locus_tag="VFY96_17655"
     tRNA            6531..6605
                     /locus_tag="VFY96_17655"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6563..6565,aa:Thr,seq:tgt)
     gene            6688..6772
                     /locus_tag="VFY96_17660"
     tRNA            6688..6772
                     /locus_tag="VFY96_17660"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6722..6724,aa:Tyr,seq:gta)
     gene            6781..6856
                     /locus_tag="VFY96_17665"
     tRNA            6781..6856
                     /locus_tag="VFY96_17665"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6814..6816,aa:Gly,seq:tcc)
     gene            6920..6992
                     /locus_tag="VFY96_17670"
     tRNA            6920..6992
                     /locus_tag="VFY96_17670"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6953..6955,aa:Thr,seq:ggt)
BASE COUNT         1812 a         1713 c         1966 g         1568 t
ORIGIN      
        1 cagatgcgcg cgattcagaa ggaactcggc gaaaaggacg agttcaagaa cgaaattcaa
       61 gaaatcgaag aaaaattgaa gcaaaagaaa ctctccgccg aggccaaaga caaggtagaa
      121 aaggagctga agaagctcaa aatgatgtct cccatgtccg ccgaggcgac ggtggtgcgt
      181 aactatatcg attggatcat cagcctgccc tggaacgact ttacagatga caagctcgac
      241 atcaacgaag cggagcgagt cctcgaggac gaccactacg ggttggaaaa ggtgaaagag
      301 cgtatccttg agtaccttgc cgttcagagc ttggttggca agataaaagg tccgattctc
      361 tgtctggtag gccctccagg cgtcggcaaa acttcattgg gccgatcgat cgcccgggcg
      421 acgggaagaa aattcgtcag ggtctcactc ggcggcgtcc gggatgaggc tgagattcgc
      481 ggtcatcgcc gaacgtacat tggcgctctt ccaggaaaga ttatccagtc gatgaagaaa
      541 gccggttcga gcaatccggt ttttctcatg gacgagatcg acaagatgtc cacggatttt
      601 cgcggcgatc catcgtcagc cctgctcgag gtcctcgacc cggagcaaaa taccgcgttc
      661 aacgatcact acctcgattt ggattacgat ctttccaagg tcatgttcat taccacggcg
      721 aataccttgg atcgaattcc gcgccctttg caagatcgaa tggagatcat tcgtattgcc
      781 gggtacacgg aactggaaaa attgagcatc gccaaaaagt accttctgga taaacaaaaa
      841 gaggcgaacg gactcaccgc agacaatttg gttttcacgg ataatgcaat cttaggtgtg
      901 gtgcgccact atacgaagga ggcaggagtt cggagcttgg agcgcgagat tgctgcaatc
      961 tgccgaaaag tggcggtcga agtggtgcgc cgcgaccgta acgctcatat ccaaattggt
     1021 tcgaagagtt tacataaata tcttggtccg gtgaaattcc gatacgggaa agccgagacc
     1081 gaggtaaaaa ttggggttac taccggcttg gcctggaccg agttgggtgg tgagctgttg
     1141 gcaaccgagg tgacgatcat gccaggcaaa ggccagctga ttatcaccgg caagttgggt
     1201 gacgtgatgc aagaatccgc acaagccgcc atgagctatg tgcgctctcg cgccaccgaa
     1261 cttggccttg caaaggattt ctaccaaaaa ctcgacgtgc atatccatgt gcccgaaggc
     1321 gcaatcccga aggacggtcc ttccgccgga atcacgatgg cgacatcgct ggtttcggct
     1381 ttgatgaaac ttccggtgca caatgatttg gcgatgactg gagaaattac cttgcgagga
     1441 actgttttgc ccatcggtgg gcttaaagaa aagatcctcg ctgcgcatcg cgctggcatt
     1501 aaaaaagtgc tcatccctgc agagaacgaa aaggatatag aggaaatccc agcaacggtg
     1561 ctcaaaacgg tcgagctcga attggtgtct cacatggatc aggttctgaa aaaggcgtta
     1621 gtgttggacg atccagagag cctcttcaga cgcgagccgt tggaaaatca agccaaagac
     1681 gaacctcctc cctttgccga aaaggtcgac gacgtacccg caagcgagat tctgccgcaa
     1741 taattcgttc gccgaatcga aaccggtgcc gggtggtttg acacccggca tttcggctgt
     1801 tataaaacca catcgcgctg tgacccgaat aattgcgggg atcccagcgc gctttttcat
     1861 gatgggcttg ccatctgaaa tcacgctgct caataaatcg ccgatgagct cgcggtccat
     1921 agcaattgag gggaagacct cagtgatcgt gaaagccggt gttagtagcc aagggcggtt
     1981 agctcagcgg gagagcatcg gccttacaag ccgggggtca caggttcaaa tcctgtaccg
     2041 cccaccatgg ggtaaagcgc ttggtttatg tcggggtcgt agttcagctg gttagaacgc
     2101 cggcctgtca cgccggaggt cgcgagttcg agtctcgtcg gccccgccat tactcgaatc
     2161 taacgggcta ttttccggtc ctggagtctg caggcgatga agaaacaaga agcgctgtcc
     2221 caaagacgct ggtatgtgat tgacggtcaa ggcaaaattc tcggtcgcat ggccactgaa
     2281 atcgcccggg tactgcgcgg aaaacacaaa cccacattta ctcccaatca agacaccgga
     2341 gactttgtca ttgtcatcaa cgctcgcggg gtgaaactga ccggcgctaa gctacaagac
     2401 aaaatctatt atcggcacac cgaatttccc ggcggtattc gcgagcgaac cgcggcggag
     2461 atgctgcaaa tcaagccgga ggagttggtg aagttggctg tccggggaat gctcccgaag
     2521 aatcgtttaa gtcgtaagct ggtgaccaag ctcaaggttt atgccggccc agagcatccg
     2581 cacacggcgc aaaaaccgga acctttgacg attgcgagct aggagaatct gatggcagaa
     2641 aaggtgttta cggcaacggg tcggaggaaa acttcggttg cgcgcgtttt cctgaagcct
     2701 ggcaaaggca gaattacgat caatgcgaaa tcgttgcagg agtatttcgg ccgggagact
     2761 gctcgcatga ttgttctgca gccatttgat gtgacgcaaa cgtccggaaa ttttgatatt
     2821 gatgtgaatg tcgtcggcgg cggaaattcg ggtcaggcgg gtgccattcg ccacgggatc
     2881 acgcgggctc tgatgcaaat caatcccgag ctccggtcac ctctgaaaaa ggccgggttt
     2941 gtcacgcgtg acccacgcgc agtcgagcgg aaaaagtacg gacgccacaa ggcgagaaaa
     3001 cgcccccaat actcaaagcg ctaagaccat tcccgccgca aagctttgcg ggtctgctat
     3061 gtctcgaagt atcctgagat ttagtagaag gcgcgcggaa gctcctaaat tagtcctaac
     3121 accctaagtg ctcgaagttg tttcaacggt atttgctgtt ggtcactcct ctgccgtgaa
     3181 gaagaatagc ccaatcatcg cgaagattgc gtttggcgcc caggcggata ttaacgaagg
     3241 catggcgcca ctgtggccga gcgagagaca aaacgcagct aaaacccaat agccaaacgc
     3301 gatcaccatc gcgatcccaa agctcagcga gagagtcccg gtcatctttc tcttgatcgc
     3361 gaatggaatc gccagcaaaa ccattagcgg cgagatgaac ggcagggcga gtttcgtctg
     3421 aagatcgact tcgtaatagg taggatcgac gcctttggct ttcatatcgg cgatctgttt
     3481 ttgtaaatcg aaaaacgtga attcctcggc atcccgcgcc agcagctgaa gatctttcgg
     3541 agtttcggag agcggcagcg tcgttgtggt ttcccgctga accatctttc cgtccgggag
     3601 cagagtccat tcggtggcgc cggtggcatt ccaaccgttc tgggtgtatt gggcgcgtgg
     3661 aatttcgagc agtccacgca agctaaagtc acgattgagg agaaacaccg tcacattctc
     3721 gaggacattc gtgcgggggt caaaattatc aatattgata aagctatttt cggcacgtat
     3781 ccagatatcg tgagttccta gcagactttg ctgctgtttg tttttgatct ctgtcttgta
     3841 aatggtttgg gcgttatgag caaagacagg gaccaaggct tcgttccaca gaaagtttag
     3901 cgcacaaacg attaacgaca gaagcagcaa gggtagagca attcgctgaa ccgtgattcc
     3961 acttgctcgc attgcggtga tctcgtgggt gcgggtcaac gcgccgagac agaaaagtgt
     4021 cgaaaaaaga acagcaaagc cgatcacgcg agagatcaat aacggcgcct tgtaaaaaaa
     4081 ataccgtaaa atagtgaaaa gtggcgcgcc ggcatcgagc agagcgccga ttcgttcaaa
     4141 gaagtccacg gttgtataaa ccaacgtgat cactagcaaa ctgatgaaga aaatccgtaa
     4201 aaagttgctg gcgacgtagc ggtccaaaat tgcgccgacg ggaaggaatt tgaaagcgag
     4261 ccgtcccatc agttgtgctt tctatggaga cttctcctga gctcaaccca atcacgtgcg
     4321 attgattcgg cagcacggtc aagcactcgg ggaaaactca gcggtgaatc gcgcaatgca
     4381 tgtcggaaga aatgcgctcc aatagcaccg actacaaggt tcggcagcca cagagcaggg
     4441 atgggattaa gaatattttt gtcgcctaga gcctttccga gtgaaagcag ggcatagtag
     4501 gccaccagcc aaaagagaca caacataaat ccccacgacc ggttggcgcg agaggagcgc
     4561 gggaccagag tcagcgcgac tcccagaagg caaaatagta aaggaacaaa tccgaaagaa
     4621 atacgctggt gcagttccat tcgctccgcg atggttttgg cacccgctcg ttgttttttt
     4681 tcgatgctcg acagcaactc cgagacggtc atttctttcg gacccgattc cttctgctga
     4741 atcggaccga taagatcgtc gaggtcgagc ttgaaatcat aaatgttgaa acgcgtttgg
     4801 ctgaaaccgg gtcgattttt ctctcgttca tagatactgc cgtcaaacag tcgcagaccg
     4861 agggtgttgg tctcctcttc cgtggagata cgcgcaacct ttcccaagat gatatcctcc
     4921 cggctctcgt ctcgcttatc gacaataaga accccttggg cggtattgcc gggtggaatg
     4981 atctcctcga cgtagattaa aattttggga aaatcgtcgt taaatatctt ttccttgagg
     5041 gccgtgccaa cccgattctt ggcgatattg taaagctctt tttttaatgc aaagttcgcc
     5101 acggggcgcg cgaacagggt gagaaagaaa gtgatgagcg cgatcacaga tgccaaagcg
     5161 acgacaggcc aaagaatttg cgccgggctg atacccgatg ctttcatcgc caggagttct
     5221 tggtcattgg acattcggcc cagtcctaag agaatggcga gtagaaaggc catgggcacc
     5281 gtcatttcca aaaaggtggg aagaatcaaa gagaacaact tgaggatctg taaaattggc
     5341 acaccacgtg tcacgaccaa ttcgataagc ctcaagatac gccctatcaa cagcaccgaa
     5401 gtgaacgcga gcaagcccaa taaaaacggc ggaatgattt cagagatcaa gtagcggaac
     5461 agaattcttc gcttcacgtc gaggcatgtt aacgagtgcg atacggtttg taaaatcctc
     5521 gagcgcgaaa ttgcaagata gctagccatg tcgtcacgca ccgaaataat ttttggtatc
     5581 aatccagtgc aggaaaaggt tcgtgcctcg ccgaaagacg tcttagaaat tttgtttgcg
     5641 gaagcgggtg atcgcgccgc tttgcgctcg atcgagcgcg aagctgtccg ccatggagtg
     5701 cgcgtctcgc gtgtgccggt tcatcttctg gatcgattgg cctcaggtca gcgacatcag
     5761 ggtgtcatcg caaaaatcga gtgctttcgt tacctgtcgc ttgatcaatt cctggagcgg
     5821 atctcagcgg cagtcaactc agaatggatt ttaattctcg atggtgtgac cgatccgcgt
     5881 aattttggcg cattgctacg aacggcggaa gcggtcggaa ttgggcacgt cgttatacca
     5941 aagcatcgct cagccgaagt gagcccgctg gtcgtgaaga catcatcggg agctacgttt
     6001 tatctgaata ttgttaaagc gacgaatctg cgaagggcgc tctgggagct aaaaaagcgt
     6061 ggttactgga tcgtggggct ggacgcgaaa gcgaccaaga ctatctacga aaacaactac
     6121 ccggaacggc tggtcatcgt cctgggaagc gaaggcaagg gcattcgcca gattaatttg
     6181 cgggaatgtg attttgtcgt ttcgattccc atgctcggca aagttgcctc cctgaacgtc
     6241 gcagttgcgg gagccgtttt tttgtacgaa gttttgcgac aaaggcaggc gggcgtggtg
     6301 gagcactagc ggatgcgctc tggatggttg ttgatacgac ctacctagct attgacatga
     6361 ttacttgaag gtgctttaaa taaccgttta cataaaagag tacgttggga tgcctcggcc
     6421 tgcgccttag agatattgct ggattttggc ggagaattgc atcgactccc cgccaggttc
     6481 tgatcgcgga ggtgggcttt ttgcgcaagc gccgggctgg cgcaagctaa gctggcgtag
     6541 ctcaacggca gagcagctga tttgtaatca gcaggttgta ggttcaaatc ctatcgccag
     6601 ctccagtcta aacggagccc gattcaggtt ctacatttgg tccgttatat caggagtatt
     6661 agtaacttaa ccgaggttaa ggggatagga gaggttcccg agtggccaaa gggagcagac
     6721 tgtaaatctg ccggcgatgc cttcgaaggt tcgaatcctt ccctctccac cagacttcac
     6781 gcgggaatag ctcagttggt agagcgcaag ccttccaagc ttggggtcgc gggttcgagt
     6841 cccgtttccc gctccatctt gcgtttgtcg atggtgtacg gatcgacaac gcgggcaggt
     6901 acaatcgtcg gtgctatttg cccaggtagc tcagtcggta gagcacgtcc ttggtaagga
     6961 cgaggtcggc ggttcaatcc cgctcctggg cttgcttcga agatacggca ccggattttg
     7021 gcatcgttaa aacgattgat cgtaaatcaa taggaggaa
//
LOCUS       DASULZ010000858         5396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_301917, whole genome shotgun sequence.
ACCESSION   DASULZ010000858 DASULZ010000000
VERSION     DASULZ010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5396
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301917"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="VFY96_17675"
     CDS             <1..1023
                     /locus_tag="VFY96_17675"
                     /inference="COORDINATES: protein motif:HMM:NF023367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HEX5608355.1"
                     /translation="EQLVDAVKREAARLDRQVYLIAESADNNARLVRERERGGYGLDG
                     QWNDDFHHCVRTLLTGDLEGYYRDYGDFCQLAKAYGEGFIYSGEYSGFRRRSHGSSSY
                     DIPPRSFVVFAQNHDQIGNRRLGDRLSGAVTLEDVKLAAGLTLLSPFIPLLFMGEEYA
                     EDAPFLYFIDHSDPDLIEAVRRGRREEFGAFAWQGEVPDPQAETSFLRSKLRPELRAK
                     DQHKVLLEFYRTVIKLRKTLPALRRLDKTSMKVVAHENEKILAIERWDGADRILLIAN
                     LNTTAQTAPINAAGGAWRKLLDSAEEKWRGPGTLLDNELEGRECGRMKLAPRSFGLFH
                     CQRTSL"
     gene            1020..3230
                     /gene="glgX"
                     /locus_tag="VFY96_17680"
     CDS             1020..3230
                     /gene="glgX"
                     /locus_tag="VFY96_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEX5608356.1"
                     /translation="MKLWPGKPYPLGATWDGAGVNFSLFSENATKVELCLFDRADERE
                     TRIPMTEQTHQVWHVYLPEVRSGQFYGYRVHGPYEPQQGHRFNPAKLLLDPYAKAVTR
                     TVRWSDALFGYRIGDPEQDLSCDDRDSASYMPKCFVVDPAFSWGNDMPPAIPWHKTII
                     YELHVKGFTMRHPAVPPELRGTYAALTCPEVIDYLKALGITAVELMPVHQFIADKHLK
                     DRGLTNYWGYNSIGFFAPDARYASSGVTGEQVAEFKSMVRALHREGIEVILDVVYNHT
                     GEGNHLGPTLCFRGVDNASYYRLVPEDRRYYMDYTGCGNTLDMTHPRVLQLITDSLRY
                     WVREMHVDGFRFDLASTLARELHDVDRLGAFFDIIQQDPVISQVKLIAEPWDLGPGGY
                     QVGNFPALWAEWNAAYRDTVRRFWKGDSGQVGGLGYRLTGSSDLYGHSGRLPYASINF
                     ITAHDGFTLHDLVSYNEKHNEANGEDNRDGHNENLSWNCGAEGETDNSEVVALRGRQK
                     RNFLATLFLSQGVPMLLSGDEIGRTQKGNNNAYCQDNEISWVDWKLDKPRRELLEFTR
                     CVIRLMDQHPVLRRRHFFQGRRIRGSEVKDLAWFRPDGKEMNDEDWNNPETRCFGLRL
                     AGDAIEELDTRGNRIVDDTLLILLNAYHEAIPFTLPAHRRKVRWEVVMDTNDSQYGKT
                     AHRAMRGGDTYELKARSLALLRLPKPLEGENGAESRVVSRRARRNGAALSEAPL"
     gene            3227..>5396
                     /gene="treY"
                     /locus_tag="VFY96_17685"
     CDS             3227..>5396
                     /gene="treY"
                     /locus_tag="VFY96_17685"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEX5608357.1"
                     /translation="MRIPAATYRLQFTPDFGFREAEAVLPYLHELGITDIYASPIFYA
                     RPGSQHGYDVVDPNQLNPELGTDQQFQSLSDSAQRIGFGWLQDIVPNHMAYDGRNRML
                     MDVLENGQSSPFFDFFDIDWQHPYESMHAKVLAPFLGGFYGECLENGEIQLYYDERGF
                     GIRYFSLELPLNVESYSDLLTYNLYSLRKRLGAQDIDFIKFLGVLYALKNIPATQEND
                     DRADQILFVKRMLWELYTGSPEIKKHFDVNLATFNGVAGNPESFNLLDRLLSQQHYRL
                     SFWKVAAEELDYRRFFNINELISLKIQNEKVFQYTHALILKFVREGKFSGLRVDHVDG
                     LYDPLGYLSRLRRETDDIYLTVEKILGADENLPISWPVEGTTGYDYLNALSGIFCARE
                     NQQKLSHIYERFTGLHTSGWSVITEKKRLIIGKYMAGDIENLAYLLKRVSSRDRHAVD
                     VTLYGLKRALVEVLAFFPVYRSYVTSASFSTQDREQLTRAVREAREANAGLRLELNYI
                     ERFLLLDFTEHSTAQERECWTEFVMRFQQLTGPLMAKGAEDTAFYVYNRLLSLNEVGG
                     EPDRFGLSIKEFHNFNERRGAHWPHAMNASATHDTKRGEDARIRINVLSEVPAEWEKN
                     LRSWSRINRGKKTTVRGAEAPDRNDEYFLYQTLIGSYPADKPHDSQFEERLAAYLIKA
                     VREAKVHTEWLQPDTAYEKAFIDFARQILARGESPFMNEFT"
BASE COUNT         1214 a         1499 c         1521 g         1162 t
ORIGIN      
        1 gaacagctgg tcgatgcggt aaaacgggaa gcggctcggc tcgaccggca ggtttatctg
       61 atcgccgaga gcgccgacaa taatgcgcgt ctggtacgcg agcgcgagcg cggtggatat
      121 ggtctcgatg gtcagtggaa cgacgacttt catcactgcg tgcgcacgtt gctaaccgga
      181 gatctcgagg gctactaccg agattacggc gatttctgtc aactggcaaa agcctacggc
      241 gaagggttca tctattccgg tgagtattcc ggatttcgcc ggcgcagcca cggcagcagc
      301 tcgtatgata ttcctccccg gagcttcgtt gtgttcgccc aaaaccatga tcaaatcggc
      361 aatcgccggc ttggcgaccg tctgagcggg gcagtgacgc tggaagacgt caagctcgcc
      421 gccggcttga ccctgctctc gccgtttatt ccgctgctgt tcatgggtga agaatacgcc
      481 gaagatgccc catttctcta tttcatcgac cattccgatc ccgacttgat cgaagcggtc
      541 cggcgcggcc gccgcgagga gttcggcgcc ttcgcatggc agggggaagt tcccgatccc
      601 caggcggaaa cgagttttct tcgttccaag cttcgcccgg agctccgcgc taaagatcag
      661 cacaaagtct tgctcgagtt ttaccgaaca gtaattaagc ttagaaaaac cctgccggca
      721 ctgcggcgcc tcgacaagac gagcatgaaa gtcgtggcgc acgagaacga aaaaattttg
      781 gcaattgaac gctgggacgg cgcggaccga attctgctta tcgccaatct caacacgacg
      841 gcgcagaccg cgccgattaa tgctgccggc ggcgcttggc gcaagctcct cgattccgcc
      901 gaggaaaaat ggcgcgggcc gggcaccttg ctggataacg agctcgaagg tcgagaatgc
      961 ggccgtatga aacttgcgcc gcggtcgttc ggattatttc actgccaaag gacatcatta
     1021 tgaagctatg gccaggtaaa ccgtaccctt tgggcgcgac gtgggacggc gcaggagtca
     1081 atttttcgct tttttccgag aacgcgacca aggtcgaact gtgcttgttc gatcgcgccg
     1141 acgaacgaga aacccggatt cccatgacgg agcagaccca tcaggtctgg catgtttacc
     1201 ttccggaggt gcgctccggg cagttttacg gctaccgcgt gcacggcccg tacgagccgc
     1261 aacaaggtca ccgcttcaat ccggcaaagc tcctcctcga tccctatgcc aaagcggtta
     1321 cgcggacggt gcgctggagc gacgctttat tcggttaccg gatcggcgac ccggaacagg
     1381 atctttcctg tgacgatcgc gacagcgcct cgtacatgcc gaaatgtttc gtcgtggatc
     1441 ccgcgttcag ttggggtaat gacatgccgc cggcgattcc atggcataag acgattatct
     1501 acgagcttca tgtgaagggc tttaccatgc gtcatcccgc tgtgccgccg gagttgcggg
     1561 gtacttatgc ggcgcttacg tgtccggagg tgattgatta tctgaaggcg ctcggcatca
     1621 ccgcggtcga gttgatgccc gtccatcaat tcattgccga caaacatctg aaagaccgcg
     1681 gcctcaccaa ctactggggt tacaattcca tcggcttctt tgccccggat gcgcgctacg
     1741 ccagcagcgg cgttaccggc gaacaggtgg cagagttcaa gagcatggtg cgagcgctgc
     1801 accgggaagg tatcgaggtg atcctcgacg tggtttacaa ccacaccggc gagggcaacc
     1861 acttgggacc gacgctttgc ttccgcggcg ttgataacgc ctcatactat cggctcgtgc
     1921 ccgaggaccg gcgctactac atggactaca cgggctgcgg caatactctg gacatgaccc
     1981 atccgcgcgt gctccagtta atcaccgata gcctgcgcta ctgggtcaga gaaatgcacg
     2041 tcgacgggtt tcgtttcgat ctggcctcga cattggcgcg cgagctccac gacgtcgatc
     2101 gcttgggcgc atttttcgat atcattcaac aggatcccgt catttctcaa gtcaagctga
     2161 ttgccgagcc ttgggatctt ggtccgggag ggtaccaagt cggcaacttt ccggctcttt
     2221 gggccgaatg gaatgcagcc tatcgggata cggtgcggcg gttttggaaa ggcgatagcg
     2281 gccaagttgg cggtctggga tatcgactga ccgggtcgag cgatctttac ggccacagtg
     2341 gtcggttacc gtacgcgagc atcaacttca tcaccgcgca tgatggtttt accctgcacg
     2401 atttagtcag ttacaacgaa aagcataacg aggccaatgg cgaagataac cgcgacggcc
     2461 acaacgaaaa cctgagctgg aactgcggcg ccgaaggaga aaccgacaac tcagaagttg
     2521 tcgcgctgcg cgggcggcaa aaaaggaatt ttttggcgac cttgtttctg tcccaaggcg
     2581 tgccgatgct attgtcgggc gacgaaatcg gccgcactca gaagggcaac aacaacgctt
     2641 attgtcagga caatgaaatc tcctgggtgg attggaagct cgacaagccg cgccgcgagc
     2701 tcctggagtt tactcgctgc gtgattcggt tgatggacca gcacccggtg ctgcgccggc
     2761 gccatttttt tcaaggccgg cggattcgcg gctcggaagt caaagacctt gcctggtttc
     2821 gccccgacgg caaggagatg aatgatgaag attggaacaa tcctgaaacc cgctgtttcg
     2881 gtctgcgtct cgccggcgat gctatcgagg agctggatac gcggggcaac cgtattgtcg
     2941 atgatacact gctcattttg ttgaacgcat accacgaggc gatcccgttt actcttccgg
     3001 cacaccgccg gaaagttcgt tgggaagtgg tcatggatac caatgactct cagtacggta
     3061 aaaccgcgca tcgcgcgatg cgcggcggcg atacctacga gctaaaagcg cgatcgctgg
     3121 ctttgttgcg gctgccaaaa cccttggaag gagaaaacgg cgccgaatct cgcgtcgtgt
     3181 cccgccgcgc ccggcgcaac ggcgcggcac tgagcgaagc gcccctatga gaatccccgc
     3241 ggcaacttac cggctccaat ttacgccaga cttcggtttt cgtgaagccg aagcggtgtt
     3301 gccctatttg catgagctcg gcatcaccga catttatgca tcgccgattt tttatgcccg
     3361 gcccggaagc cagcacggct atgacgtcgt cgatcccaat cagctcaacc cggagcttgg
     3421 caccgaccag cagtttcaat ccctgagtga ttccgcccaa cggattggct tcggttggct
     3481 tcaagacatc gtgcccaatc acatggccta tgacggccgt aatcgcatgc tgatggatgt
     3541 tctggaaaac ggccagtcat caccgttttt cgatttcttt gatatcgatt ggcagcaccc
     3601 ctacgaaagc atgcacgcca aagtgctggc gccgtttttg ggcggcttct acggagaatg
     3661 cctggagaac ggagagatcc agctctacta tgacgagcgc ggctttggca ttcgctattt
     3721 ttctttagaa ctgccgctta acgtcgaatc ctacagcgat ctgttgacgt acaatctata
     3781 ttcgctcaga aaacgtctgg gcgcccagga tattgatttc atcaaattcc tcggcgttct
     3841 ttacgcgctc aaaaatattc cagcgacaca ggaaaacgac gaccgggctg atcaaatctt
     3901 gttcgtcaag cgcatgctct gggagctcta taccggatct cccgaaatca aaaaacactt
     3961 cgacgtcaat ctggcgacgt tcaatggagt agcgggcaac cccgaaagct tcaatcttct
     4021 cgaccgcttg ttgtcgcagc aacactatcg tctttcgttc tggaaagttg cggcggagga
     4081 gctcgattat cgaaggttct tcaatatcaa cgagttgatc tcgctcaaga tccaaaacga
     4141 aaaggtcttt cagtacaccc acgcgctcat cttaaaattt gtccgcgaag gcaagtttag
     4201 cgggctgcgc gttgaccacg tggacgggct ttacgatccg ctcggctatc tcagccgact
     4261 ccgacgagaa accgacgata tctatctgac cgtagaaaag atccttggtg cagacgaaaa
     4321 tctcccgata tcctggccgg tcgaaggcac aacgggttac gactacctca acgcgttgag
     4381 cgggattttt tgcgcgcgcg agaatcaaca gaaattaagc cacatatatg agcgcttcac
     4441 cggattgcac acgtcgggat ggtccgtcat cacggaaaaa aaacggttga tcatcggcaa
     4501 gtacatggcg ggagacattg agaatctcgc ctacttgttg aaacgggtat cgagccgcga
     4561 ccggcacgcc gtcgacgtga cactttacgg cctcaagcgg gccttggtcg aagtgctggc
     4621 gttcttcccg gtttatcgaa gttatgtgac atcagcctca ttcagcacgc aagaccggga
     4681 gcagctgacc cgggcagtca gggaagcccg cgaagccaat gccggcctgc gtctggagct
     4741 gaattatatc gaacgctttc tgcttctcga tttcaccgag cactcgaccg cacaagagag
     4801 ggagtgctgg acggaattcg ttatgcgctt ccagcaactg accggacctc tgatggcgaa
     4861 gggcgcggaa gatactgcat tttatgttta taaccgtttg ctctcgctga acgaggtggg
     4921 tggtgagccc gatcggtttg gcttgtccat caaggaattt cataacttta atgagcgccg
     4981 cggcgcgcac tggccgcacg cgatgaacgc cagcgcaacc cacgacacca aacgcggtga
     5041 agacgcgcgg attagaatca acgtactctc ggaagtcccc gctgaatggg aaaaaaatct
     5101 gcgcagctgg agcaggataa accgcggcaa gaaaaccacg gtgcgaggcg ccgaagcgcc
     5161 ggaccggaac gatgaatact tcctttatca aacattgatc ggttcttacc cggcggacaa
     5221 accgcacgat tcgcagtttg aagagcggct ggcggcttac ttgatcaaag cggtcagaga
     5281 ggcgaaggtt catacggaat ggttgcagcc ggacaccgca tatgagaagg cttttatcga
     5341 tttcgctcgg caaatccttg cgcgcggcga aagcccgttc atgaacgaat ttactc
//
LOCUS       DASULZ010000859         2925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1112498, whole genome shotgun sequence.
ACCESSION   DASULZ010000859 DASULZ010000000
VERSION     DASULZ010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112498"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VFY96_17690"
     CDS             complement(<1..553)
                     /locus_tag="VFY96_17690"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5608358.1"
                     /translation="MGIAATSVGFLPIYAAYHRGFFKDEGIDLEIILMSLAAANNAFF
                     KGEIDYSAGLTGLALAAVRNYPAKILIFTVAKPLQSFMSRREIKEPRDLRGKRVAGSS
                     PGGSATILAYQALKHFGLEPGKDVQVLPMGGSGAGRLAVLEQGVVDASLLSVPENILA
                     LHKGYNELIFLGDVLSFPQNGFGT"
     gene            complement(849..2024)
                     /locus_tag="VFY96_17695"
     CDS             complement(849..2024)
                     /locus_tag="VFY96_17695"
                     /inference="COORDINATES: protein motif:HMM:NF023431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HEX5608359.1"
                     /translation="MTSDPRTRTLSQTCAERGPNRPLHTKGGSPNYLEKSLAAAAAPF
                     KGITTNGQVIPGLFGIQKTGVSTQTMREAADAFLDSLSAEQRAKTLFPIDTDQWRKWS
                     NIHPTLMRHGTPLFELNDHQRDCAFALMRESLSQNGFEEALNVMHLNETVLEMTGRLS
                     EYGEDLYWLSIMGKPSSTEPWGWQWDGHHLILNYFVLGDQVVVTPSFLGAEPVHAKTG
                     KYAGLRIFQNDEERGLTFARSLSEAQRGQAVIAPETSGEDLTTAFRDNLVLDYAGIRY
                     EALSGGQHDLLLDLIGYHVGRMRSGHADIKMKEVKDHLRDTHFCWMGGMADDSTFYYR
                     VQSPVIIIEFDHQRGIAFREHTKPYKDHIHVIIRTPNGNDYGKDLLRQHYQFGHHAK"
     gene            complement(2103..2306)
                     /locus_tag="VFY96_17700"
     CDS             complement(2103..2306)
                     /locus_tag="VFY96_17700"
                     /inference="COORDINATES: protein motif:HMM:NF026048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX5608360.1"
                     /translation="MNSPMKVRIVESRCIACGLCREVCPEHAVHPKMQDIHHMYEIVE
                     AECTGCGDCLPYCPEPGALEQYG"
     gene            complement(2303..>2925)
                     /locus_tag="VFY96_17705"
     CDS             complement(2303..>2925)
                     /locus_tag="VFY96_17705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608361.1"
                     /translation="VTQAIQAAEKDLNSGSDEYKAVRARAGYAAEGPFMSLKQMSTQI
                     LSVEIDEQPKYIDLQRMRARQIWDEVKHGQLHADVLLRGGFISREEELMDDPRANTQP
                     KLSYFGMTAMFPHIHPLARAGQHYYNESMACLGISATLSIIDDDLVRHQLYSQKAEEL
                     MHFMEGKYQIDAYALTPQDQKPIEEVFDFLLRPWAPEPSRALGGSQ"
BASE COUNT          622 a          803 c          820 g          680 t
ORIGIN      
        1 atgtgccgaa tccgttttgc ggaaaagaaa ggacgtcgcc caaaaagatc agctcgttat
       61 agcctttatg cagcgcgagg atattctccg gaaccgaaag cagcgaagca tcgaccacgc
      121 cctgttcgag caccgccagc cgcccagctc ccgagccgcc catcggcagc acttgcacgt
      181 ccttgccggg ctcgaggccg aaatgcttga gagcttgata agcgaggatg gtcgcggagc
      241 cgcctggcga gctgcccgcg actctctttc ctctgagatc gcgcggctct ttgatctccc
      301 tgcggctcat aaacgattga agcggctttg ccaccgtgaa gatcaagatt ttcgctggat
      361 aattgcgcac cgctgcgagc gcgagtcccg tgagtcccgc gctgtagtcg atctcacctt
      421 tgaagaatgc gttgtttgcg gcggcgaggg acatgagaat gatttcgaga tcgatgcctt
      481 cgtctttgaa aaagccgcgg tggtaagctg cgtagatcgg taaaaacccc acgctcgtcg
      541 cggcgatgcc caagcggatc tttctgagat tcgattgagc tctggcattt gacggcgcga
      601 tcgaagccgc gcacaagact gtgaagaaac aaagcaacgc gagggcgagc cgtttgcgag
      661 gtgacatcct gtccggacct acatcagttt taaatggatg ttgcactatg atttcgaact
      721 gcgctctttg ccgtcagctc gcgacagaag caaaagatgc ttcgctttgc tcagcgtgac
      781 acgtggaatc gttcctcatg cacgggcaag aagtgaaggc agcgcgtctg atacaattga
      841 tgacgggctc attttgcatg gtgcccgaat tgataatgct ggcgcaacag atctttgccg
      901 taatcgttcc cattgggcgt gcggataatg acgtgaatgt ggtctttgta cggcttggtg
      961 tgttcgcgaa aggcgatgcc gcgctgatgg tcgaactcga tgataatgac ggggctttgg
     1021 acacggtagt aaaaagtgct gtcgtccgcc atcccgccca tccagcaaaa gtgggtatcg
     1081 cgtaaatgat ccttcacttc cttcattttg atatcagcat ggccgctgcg catgcgtccg
     1141 acgtgatagc cgatgagatc gagcaacaga tcgtgctgcc cgccggataa tgcttcgtag
     1201 cgaatccccg cataatcgag caccaggttg tcgcgaaaag ccgtcgtcaa gtcctcgcct
     1261 gaggtttccg gcgcaattac tgcctggccg cgttgcgctt ccgacaaact gcgcgcgaag
     1321 gttagtccac gctcttcgtc attctggaaa attctcaggc cggcatattt gccggttttc
     1381 gcgtgcacag gctctgctcc aagaaagctc ggagtcacta cgacctgatc accgagaacg
     1441 aaataattga ggatcaggtg atgaccgtcc cactgccaac cccatggctc ggttgaggat
     1501 ggcttgccca tgatactcag ccagtaaagg tcttcgccgt attcgctgag gcgcccggtc
     1561 atctcgagca ccgtctcatt gaggtgcatg acatttaacg cctcttcaaa gccattttga
     1621 ctcaagcttt cgcgcatcag cgcgaaagca caatcccgct gatggtcgtt caactcgaac
     1681 agaggcgtgc catgccgcat gagcgtcgga tgaatattac tccacttgcg ccactgatcg
     1741 gtgtcgatgg gaaaaagggt tttggcgcgc tgttcggcgc taagcgaatc gagaaaagca
     1801 tcggcagctt cgcgcatcgt ctgggtcgaa acacccgtct tctgaatccc aaacaaccct
     1861 ggaataacct gcccgtttgt ggtgatcccc ttgaaaggcg ccgccgccgc ggcgagcgat
     1921 ttttctaaat agttcggtga tccgcccttg gtatgaagcg gccgattggg cccgcgttcg
     1981 gcgcaggtct gagacagtgt gcgggtccgt ggatcgctag tcattaaagg cctcctttga
     2041 cggcgctgcc cggcttcaag ggatttctcg acctcgaagc aaatttaacg tacgacggct
     2101 ggctacccgt actgctctaa agcgccgggt tccggacagt agggaagaca atcgccgcag
     2161 ccggtgcatt cagcctcgac gatctcatac atgtggtgaa tgtcctgcat cttcggatgt
     2221 acggcgtgct ctggacagac ttcgcggcac agaccgcagg cgatacagcg gctttcgacg
     2281 attctgacct tcatgggcga gttcattgtg atccgccgag ggctcgactt ggttccggcg
     2341 cccacggccg gaggagaaaa tcaaaaactt cctcaatcgg cttttgatcc tgcggcgtga
     2401 gggcgtaggc gtcgatctga tattttcctt ccatgaaatg catgagctct tcggcttttt
     2461 ggctgtatag ctggtgacgc accagatcat cgtcaataat gctcagggtc gcgctgatgc
     2521 cgaggcaggc catcgattcg ttgtagtaat gttgcccggc gcgcgcgagc ggatggatgt
     2581 gcggaaacat cgcggtcatg ccgaaatacg aaagtttggg ttgcgtattg gcgcgcggat
     2641 cgtccatcaa ttcttcttct cggctaatga atcctccgcg caacaacacg tcggcatgaa
     2701 gctggccatg cttcacttca tcccagattt gccgcgcgcg catacgctgc aagtcgatgt
     2761 attttggctg ctcgtctatt tccacggaaa gaatttgcgt cgacatctgc ttcaaagaca
     2821 tgaaagggcc ttccgcagca tagccagcgc gagcgcgcac ggctttgtat tcgtcactac
     2881 ccgagtttaa gtctttctcg gcggcctgta tcgcctgcgt gacga
//
LOCUS       DASULZ010000860         4182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_100348, whole genome shotgun sequence.
ACCESSION   DASULZ010000860 DASULZ010000000
VERSION     DASULZ010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100348"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VFY96_17710"
     CDS             <1..229
                     /locus_tag="VFY96_17710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX5608362.1"
                     /translation="FARGDQDRVAQESQGPIYDRTTEHLAYTDRGVILLRRLYKDSIE
                     AVNQGRDPLGVIRDPERNQLIRLMPHEDLLD"
     gene            362..1495
                     /locus_tag="VFY96_17715"
     CDS             362..1495
                     /locus_tag="VFY96_17715"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX5608363.1"
                     /translation="MLSREENERLTRVGAGTPAGEMLRRYWWPIAFGQDAKPKGAPVK
                     IQLLGEEFVLFRDGTERLGLMTLHCSHRGTSLEFGRVEEHGIRCCYHGWLYDTRGNCL
                     EQPAEPAGSTFKDRVKHPAYHAQDAGGLIFAYIGPEPAPLLPSYDLLVRDDGCRVVGG
                     DVEHCNWLQRAENSADGAHSIALHAAGYPNMALKRPKITWEPTRFGIKETTWIEDVPK
                     PRVSHYVFPSHVRHSAARVGESPRQVIRFRVPTDDITTTTFWIDFYPDKQGQPTQPVP
                     LSVKGFRKNQPGVYERVSDGWWNLPNREQDRVAQETQGMIADRTKEHLATSDQGILML
                     RKMISDSIDAVQQGRDPMGVTRDSSLNGPITFDSSRDAVEALV"
     gene            1595..2662
                     /locus_tag="VFY96_17720"
     CDS             1595..2662
                     /locus_tag="VFY96_17720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608364.1"
                     /translation="MRTQVLIGLAASLQLLALTIRAPARAQTLEQIVTEAKKEGEVTL
                     VASASTFGGKKGFAELENLFAKRYGFKAKVSLTGGPSFPQVAARIIAEQKAGAKSSTD
                     LYLGSDGTYVTMDQEKALEKVNWSGIFPWITKPMEIFPQQSVLIYASFHGIIYNSNSI
                     PKNKAPKNYEDLVDPALSPAWAGKMAIPSYIHWLIRVSPIWGKEKVLSYARKLATLVG
                     GRLRQGEEERIVSGEFPIMASTGGALEAMWKWQAKGAPLVGLPGSNPATSSYYQLSVP
                     KNSAHPNMAKLLVALMVTREGQSVLEKNDMRSSYLIEGTLMAKYVKSNNVKLQDPREL
                     NEVFQKQDTSLEEELTRILLK"
     gene            2958..>4182
                     /locus_tag="VFY96_17725"
     CDS             2958..>4182
                     /locus_tag="VFY96_17725"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5608365.1"
                     /translation="MSTQVLTVESPRARINWSRLSLISIAGFAALAILVLLAMIIWMS
                     LRTGVPGQVSAYTLQNYAALLSDSYTYRVMRTTLIFAAITIAVSVPLGFIFAWFIERT
                     DIPYKTVAMSLLSIGILFPTFLKAMGWVFLLHPRIGVINIFLMQLFGFSRAPLNIATV
                     AGIGFVQGMTLTPLAYVMISAALRSMNPALVEAAGVHGVSQWRTLLRIELPLVWPALF
                     SAIIWMLTVAIAAFDVPGVIGMANNIFTFSTAIYFMINPNEGLPRYGLSGAYGSIMVG
                     LSLILMIPYFRALKQSHRYQIISGKSYQSRPVLLGRWWLVSWALMGGYFLLAFVLPLL
                     AIFWVSLLPYVQVPSWQALASASLDRYSQIAFDRALLEAAGNTALLMVTVPTVIVIVC
                     SAISWIVTRSRMRWRV"
BASE COUNT          928 a         1088 c         1193 g          973 t
ORIGIN      
        1 gtttgcacga ggggatcaag atcgcgtcgc gcaagaaagc caggggccga tttacgaccg
       61 aaccaccgaa catctggcct acaccgatcg tggtgtcatt ctcttacgcc gcttgtataa
      121 ggattcgatc gaggcagtga accaaggacg cgatccgctc ggagtaattc gcgatccgga
      181 aaggaaccag ctgattcgct tgatgccgca tgaggatttg ctggattgaa gaggggttca
      241 cgccggtcaa aaaagtttga tcgttcaaaa ctgggcaggt ccgtaagatt aaagctccac
      301 ctgacgcacg cgtgggcgta gctttgatat tgacctccaa tgtgcttgcg agaggaagag
      361 aatgttgtcg cgtgaggaaa atgaacgttt aaccagagtg ggagccggca cgccggccgg
      421 tgaaatgttg cgccgttact ggtggccgat tgcgttcggc caggatgcga agcccaaggg
      481 tgctccagta aagattcaat tgctgggtga ggaattcgta ctgttccgcg atggcacgga
      541 acgtctcggg ctcatgacgc ttcattgttc gcatcgcggc acttccctcg aattcgggcg
      601 cgtcgaggaa cacggcatcc gctgctgcta tcacggttgg ctctatgaca ctcgcggcaa
      661 ttgtctggaa caacccgctg agcccgccgg tagtacattc aaagatcggg taaagcatcc
      721 cgcgtatcac gcgcaagatg ccggcgggct gatctttgcc tacatcggtc ccgaaccggc
      781 gccgctgcta ccgagctatg atctcctggt aagggatgat ggttgtcggg tggtaggcgg
      841 agatgttgaa cattgtaatt ggctgcagcg tgccgagaat tccgctgacg gcgcacactc
      901 catcgcgctt cacgccgcag gttatcccaa catggcgttg aaacggccga agatcacatg
      961 ggagccgacg cgcttcggca tcaaagagac aacgtggatc gaagatgtgc cgaagccccg
     1021 cgtttcccat tatgtctttc cctcccacgt acgtcattcc gccgcacgag tcggtgaatc
     1081 accgcgccaa gtgattcgct ttcgcgttcc gaccgacgat atcacgacga cgaccttttg
     1141 gatcgatttt tatcccgata agcagggtca gccgactcaa ccggttccat tatccgttaa
     1201 aggattccgc aaaaaccaac ccggagttta cgaacgcgtt tcagacggat ggtggaatct
     1261 tcccaaccgg gagcaagatc gggtcgcaca ggaaacccaa ggaatgattg cagatcgcac
     1321 caaggagcat ctggcaacgt cggatcaggg cattttgatg ctgcgcaaaa tgatcagcga
     1381 ctccatcgat gccgtgcagc aaggcagaga cccaatgggt gtgactaggg attccagcct
     1441 aaacggaccg attacgtttg attcgagccg tgacgcagtc gaggcgctgg tttgaaatga
     1501 gagctcgcgg atcgaagatg gagaatcgaa aatcgcgatc cgcgacactc agaagtccat
     1561 tctcggctcc ttgtaagaag gcgaactctt ttagatgcgt actcaagttt taatcggttt
     1621 ggctgcttcg ctgcagttac tggcgctgac gattcgcgcg ccggcgcggg cgcagacact
     1681 cgagcaaatt gtcacggaag cgaaaaagga aggagaggtg acgctggttg cgagcgcctc
     1741 gactttcggc ggcaagaagg gctttgccga gctggaaaac ttatttgcga aacgatacgg
     1801 cttcaaagcc aaagtgtctc tgacgggtgg gccgagtttt cctcaagtgg cggcccgcat
     1861 catcgccgaa caaaaagccg gtgcgaaatc gtcgacggat ctatacctcg gctctgatgg
     1921 aacctacgtc accatggatc aagagaaggc gctcgagaaa gtaaactggt cgggaatttt
     1981 tccgtggatc accaaaccga tggaaatctt tccgcagcaa tcggtgctga tctatgcttc
     2041 ttttcacggc attatctaca attcgaattc cattcctaaa aacaaggcgc cgaagaatta
     2101 cgaagatctt gtcgatccgg cactttctcc ggcttgggcc ggtaaaatgg cgattccttc
     2161 gtacatccac tggctgatcc gagtttcgcc gatctggggt aaagaaaagg ttctgagcta
     2221 cgcgcgaaag ctcgccactt tggtgggcgg gcgattgcgg caaggggaag aagagcggat
     2281 tgtgagcggc gaattcccga ttatggcgag caccggcggc gcactggaag cgatgtggaa
     2341 gtggcaagcg aagggggcac cgctcgttgg cttgcccggg tctaacccgg cgacaagctc
     2401 gtactatcaa ttatcggtgc ctaaaaattc ggcccatccg aacatggcaa aattgctcgt
     2461 cgcattgatg gtgacgcgag aaggccaatc cgtgctcgag aaaaatgata tgcggagctc
     2521 ctatctcatc gagggcacat tgatggcgaa atatgtcaag agcaataatg tgaaactgca
     2581 agatccgcgc gagctcaacg aagtctttca gaagcaagat accagtctcg aagaggagtt
     2641 aacgagaatt ttgctgaagt agaatagttc ggtaactcgc ttgggcggct aggtcatgag
     2701 ggcgaatcat cagcttgcgc tcgcccacaa agatgaaatc tgcgctcgtt tccagctcgt
     2761 cacccccgaa tttttaatcg ggggtctgga ttcccgctaa aagcatgcgg gaatgacgaa
     2821 actgaaacgg cagcgttaca caatctttct tcggaggagg acacgaaggc tacgcagtaa
     2881 agtcatcgct tcgtgatctt cgtttgatac cagcattcgt ctgcatgttg gatcatcggc
     2941 gagccaatcc tcaaatcatg tcaacgcaag ttctcaccgt agagtcgccg agagcgcgca
     3001 tcaattggtc gcggctttcg ctgatttcga ttgctggatt cgccgcgctg gccattcttg
     3061 tgctcctcgc aatgatcatt tggatgagcc tacgcacggg cgtgcctggg caggtttccg
     3121 cttacacttt gcaaaattac gccgcgctcc tgagcgactc atatacctat cgggtaatga
     3181 ggacgacctt gatctttgcg gcgatcacga tcgctgtgtc agtgcccttg gggtttatct
     3241 ttgcctggtt tatcgagcgc accgatattc cgtacaagac cgtggcgatg agcctgctca
     3301 gtattgggat cctatttccg acgttcctca aagcgatggg ctgggttttt ctgctgcacc
     3361 cacgcatcgg cgtcatcaat atttttttga tgcagctttt cggtttcagc cgagcgccgc
     3421 tgaatatcgc gaccgtggcg ggtatcggat ttgttcaggg catgacattg acgccgctgg
     3481 cctacgtgat gatttcggcg gcgttgcgca gcatgaatcc ggccttggtt gaggccgccg
     3541 gtgttcacgg cgtgagccaa tggcgcacgc ttctgcgcat tgagttgccg ctcgtttggc
     3601 cggcgctgtt ctcggcgatc atctggatgc tgacggtggc gatcgcggct tttgacgtgc
     3661 ccggcgtcat cggtatggcc aataatattt tcaccttcag cacggcgatc tatttcatga
     3721 tcaatccgaa tgaaggcttg ccgcgttacg ggctcagcgg cgcttacggc agcatcatgg
     3781 tcgggctgtc cttgattctc atgattccat acttccgcgc tctcaaacaa agtcaccgct
     3841 atcaaatcat atccggaaag tcctatcagt ctcggcctgt gctgctcggc cgttggtggc
     3901 tcgtctcatg ggccttgatg gggggatatt ttctgctggc attcgtcttg ccactgctgg
     3961 cgattttctg ggtatcgttg ctgccctacg tgcaagtgcc ttcatggcaa gcgctcgcaa
     4021 gcgcatcctt ggaccgttac agccaaattg cattcgatcg agcgctgctg gaagcggcag
     4081 gaaacacagc gcttttgatg gtgaccgtac cgacggtgat cgtcatcgtc tgctcggcaa
     4141 tttcctggat cgtcacccgg tcgcgcatgc gttggcgggt cg
//
LOCUS       DASULZ010000861         2500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1112666, whole genome shotgun sequence.
ACCESSION   DASULZ010000861 DASULZ010000000
VERSION     DASULZ010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112666"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..566)
                     /locus_tag="VFY96_17730"
     CDS             complement(30..566)
                     /locus_tag="VFY96_17730"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX5608366.1"
                     /translation="MAQLKCPNCGREFVRRVSRSGFLEILLSYFYIYPFKCQLCGERF
                     RHCQRGVRYVRIEKDRREYDRMEISFPVTFFGQDISGEGTVINVSMGGCTLRTHTKLE
                     TGAILNLSLQISKDVPAVVVEAGVVRGMRAEIAGIEFLLWQQSERERLQLFVRGLLIG
                     RGIDLDPLVNRSEPVVPG"
     gene            complement(630..1619)
                     /locus_tag="VFY96_17735"
     CDS             complement(630..1619)
                     /locus_tag="VFY96_17735"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5608367.1"
                     /translation="MSALAYGQTPFFQGKTITIVVGYQAGDGYDIWARLLAAHMGKHI
                     AGNPGLIAQNMPGAGSMIAANHIFNVAKADGLTMGAIGPSLYLDQLVGKKEVHFDWGK
                     FGWVGSTESTPWLLYMSSNTPYRTIDDVRKAKEPPKCSATGTGTSGHFIPKLLEDALG
                     AKFNVVMGYQGGAEQDLAFERGEVVCRSLSIPTFFAREPFLTWRKKNMVRILLQTGRK
                     RDVKMPDVPTIHELMNDYKSSPTSRSLVTAVLASGDLGRPFITPPAVAPERLKILREA
                     FRKTMSDPAFLADAKARKLEPDPDFGEDLETIAKDSVSQPKEIVERMKKLLSE"
     gene            complement(1735..2316)
                     /locus_tag="VFY96_17740"
     CDS             complement(1735..2316)
                     /locus_tag="VFY96_17740"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5608368.1"
                     /translation="MTLISFTDRGKHKVVAYAIEKVESSYGHPVVGDIRVLTYYGPYR
                     DFAGVKFPTKIIQYQDGLATLDLTVTAVRANPAADIGIPDHVRNNPVPVQSEKVADGV
                     WYITGVDNNSVLIEMKDYLIVVEAPHGERRSLAVLEEIKKLVPDKPIKYLVNTHHRFD
                     HASGLRTYAAEGVTERCSANRSCLLFRSWSDLI"
BASE COUNT          559 a          705 c          652 g          584 t
ORIGIN      
        1 tagaaatcga acggcttaaa tcgcttagct tacccgggaa cgaccggctc ggagcgattg
       61 accagaggat cgaggtcgat gcctcgaccg atcagcaagc ctctgacgaa aagctgcagc
      121 cgttcacgtt cgctttgctg ccacaggaga aactcgatgc ccgcgatttc agctctcata
      181 ccgcgaacca cacccgcctc aacgacgacg gccggcacgt ctttggaaat ctgcaatgaa
      241 aggttgagaa tcgctccggt ctcgagtttg gtgtgggttc gcagggtgca acctcccatc
      301 gagacattaa tgacggttcc ttcaccggag atatcctgtc caaaaaaagt taccgggaaa
      361 cttatttcca tgcgatcgta ctcgcgacgg tctttctcga tcctgacgta acgtacaccc
      421 cgttgacaat gtcggaaacg ctcgccgcag agctggcact tgaaagggta aatatagaaa
      481 tagctcagca aaatttcgag aaaaccggag cgcgaaacgc gccggacaaa ctcccggccg
      541 cagttaggac atttcaattg agccattcca tgcctctact ccaatgtcca taggtcgccg
      601 gctttgatag gatttgcgta tgcgatactt cattcgctga gtaacttttt catccgctcg
      661 acgatctcct tcggttggga aaccgaatcc ttggcgatgg tctcgagatc ctcaccaaaa
      721 tcgggatctg gttcgagctt tctcgctttc gcgtccgcga ggaacgccgg atcactcatg
      781 gttttgcgaa aagcttcgcg aagtattttc aggcgctcag gagcgacggc gggtggtgtt
      841 atgaacggcc tgccgagatc gccggacgct aatacggctg tgactaagga gcggctggta
      901 ggggagcttt tatagtcatt catcagctca tggatggtcg gcacgtccgg cattttgacg
      961 tcgcgtttac ggccggtctg caaaagaatg cgcaccatgt ttttctttcg ccaagtaaga
     1021 aacggctcgc gcgcgaaaaa ggtgggaatg cttaacgaac ggcaaaccac ttcgcctctc
     1081 tcgaacgcga gatcctgctc ggcgccgccc tggtatccca tcacgacgtt gaacttcgcg
     1141 cccagtgcgt cctcgagcaa tttaggaatg aagtgaccgc tggtgccggt gccggtcgcc
     1201 gaacattttg gcggctcctt ggccttgcga acgtcgtcga tggttctgta gggagtattg
     1261 ctgctcatat acagcagcca cggcgtgctt tcagtcgagc ccacccaacc gaatttgccc
     1321 caatcgaagt gaacttcttt tttgcctact aattgatcga ggtagagcga tggaccaatc
     1381 gctcccatcg tcaaaccatc cgccttggcc acattgaaaa tgtgattggc ggcgatcata
     1441 gagccggcgc ctggcatatt ctgcgcaatc aagccgggat ttccggcaat gtgtttgccc
     1501 atatgtgcgg ccagcaatct ggcccaaatg tcataaccgt cgcctgcctg atatccgacg
     1561 acaattgtga tcgtcttccc ctggaagaat ggcgtctgcc catatgccaa cgcggacatc
     1621 aagcaggtcg ctagaagggt tgcacctagc gttcggacaa tcattaggtt cactttcacg
     1681 acttgtttaa atactgatgg ctccactttg agcttgcacg cacgtagcac aaggtcatat
     1741 taaatcggac cagcttcgaa acaacaagca cgatcggttg gccgagcacc gctccgtaac
     1801 gccctcggca gcataggttc gaagaccgga agcgtggtcg aaacgatggt gcgtattcac
     1861 caaatacttg atcggcttat ccggcacgag cttctttatc tcttccagta cggctaacga
     1921 acgccgctcc ccgtgaggcg cctcgaccac gatgagatag tctttcatct cgatcagcac
     1981 gctgttgttg tcgacgcctg tgatgtacca aacgccgtcc gcgacttttt ccgattgcac
     2041 cgggacgggg ttgtttcgaa catggtcggg tatcccgata tcggctgccg gattggctct
     2101 gacggcggtc accgtcaaat cgagcgtcgc caagccatcc tggtattgga tgatctttgt
     2161 tggaaacttg acgccagcga aatcgcggta cggaccgtag taggtcaaca ccctgatatc
     2221 tccgacaaca gggtgaccat acgaagattc cactttctcg attgcgtagg cgacgacctt
     2281 gtgcttgcct cgatcggtaa acgaaatcag ggtcatcggc cgcccctcga ttgtctttct
     2341 cgtaacggtc ggattgctag caaatgccgc gcagcaaacc atgaggcgag atcacgatct
     2401 catgcgcgcg ctgcggcgcc tcaaaagtct cggtgccgta tctttgccct ccccattcca
     2461 cgagtgatcg ccgctcaccg atgtcacgac tcgttgttcc
//
LOCUS       DASULZ010000862         2455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_302253, whole genome shotgun sequence.
ACCESSION   DASULZ010000862 DASULZ010000000
VERSION     DASULZ010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302253"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1210)
                     /locus_tag="VFY96_17745"
     CDS             complement(<1..1210)
                     /locus_tag="VFY96_17745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX5608369.1"
                     /translation="MDIFANVALGFSVALTPTNLLFCFLGSVVGTLVGVLPGLGPVGA
                     VAFLLSLTFKMAPATAVIMLAGIYYGAMYGGSTTSILVNIPGETASVVTCLDGYRMAR
                     QGRAGAALGIAAFGSFIAGTFGVVGLMFLAPMLGRAALRFGPAEYFALTLTGLTLVAY
                     LTRGSMIKALIMAVLGLLAGSVGMDPISGEERFTYGSLTLRDGLGLVPVAMGLFGVAE
                     VLENLAAIGTQSIYETRIKGLLPNRKDWQASGWPIARGSVIGFLLGILPGPGPVIASF
                     VSYAMEKRLSRYPEKFGTGAIEGVAGPEAANNSATAGSFIPLLSLGIPTSAIMALFMG
                     ALMIHGIQPGPLFINKYPELFWGFIASMYIGNAMLLILNLPLIGIWVRVLKTPYAILF
                     PMILLFCLIGV"
     gene            complement(1212..1667)
                     /locus_tag="VFY96_17750"
     CDS             complement(1212..1667)
                     /locus_tag="VFY96_17750"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX5608370.1"
                     /translation="MKRSQLAVSTVLAGLAGFVLLESRHLSFGTMRVPQTGFFPWILA
                     TLLLILCVILFAQGFFGAESDRRPDNILPEGWSRIAFTLAAMIGFALVLERLGFLLTT
                     FFLMILLLRAVESQRWSKVFFVAVLTAIASYAIFGWILGIPLPGGILGI"
     gene            complement(1695..>2455)
                     /locus_tag="VFY96_17755"
     CDS             complement(1695..>2455)
                     /locus_tag="VFY96_17755"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5608371.1"
                     /translation="GGGGAVGIQAARDAAPDGYTILITPPPILLIPLVNKNSGFTMKD
                     FTPLVLATSSPNTTVVKADAPWKTLEDIIAEAKKNPGALTYGSAGPGTTPHFIGELVK
                     LKTKIDLTHVPLGSESAAATALLGGHVNIAFLTLGTTRSHIEAGTMRALAVASNRRLK
                     DFPNVPTTVEKGFPELNLKIWIGFFAPAKTSGAIVKRLAAALSDTFRDPEIIGQVEKA
                     QALVENLGPQEAAKFYLEEERKWSEVASVAKIGS"
BASE COUNT          626 a          717 c          652 g          460 t
ORIGIN      
        1 agacgccgat cagacaaaat aacaaaatca tcggaaacaa aatggcatag ggcgtcttga
       61 gaactcgcac ccaaatcccg atcagcggca ggttcagtat cagcagcata gcattgccaa
      121 tgtacatgct cgcaataaaa ccccagaaaa gctccggata tttgttgatg aacagcggac
      181 ccggttgaat accgtgaatc atcagggcgc ccataaacaa tgccatgatg gcgctcgtcg
      241 gtatgccgag cgaaagcaaa ggaatgaagg agcccgccgt cgcggaattg tttgccgcct
      301 ccggcccggc aacgccctcg atggcgcctg tgccaaactt ttccggataa cgtgagagcc
      361 gcttctccat cgcataagaa acgaacgatg caatcaccgg ccccggccca ggcaagatcc
      421 ccagcagaaa gccaatgacc gagccgcgcg caatcggcca gccgctcgct tgccaatctt
      481 ttctgttcgg tagcagcccc ttgatacggg tttcgtaaat cgattgagta ccgatcgcgg
      541 ccagattttc caaaacttca gcgaccccga aaagtcccat cgccacgggc accagaccca
      601 gcccgtcgcg caaagtgagg ctcccgtatg tgaatcgttc ttcgcccgaa atcggatcca
      661 taccgacact tccggcaagt agtccaagca ctgccatgat gagcgccttg atcatggacc
      721 ctcgtgttaa ataggcgacc agggtgagtc ccgtcaacgt aagcgcgaag tattccgccg
      781 ggccgaatcg gagggcggct ctaccgagca tgggcgcgag aaacatcaga ccgaccacgc
      841 cgaatgtccc cgcaatgaac gaaccaaatg cggcaattcc caatgcggca cccgcccggc
      901 cttgacgcgc catacgataa ccgtcgagac acgtcaccac cgaagcggtt tcacctggaa
      961 tattgacaag aatagaggtg gtggacccac cgtacatggc tccgtaatag atcccggcaa
     1021 gcatgatgac tgcagttgcc ggcgccatct tgaacgtcaa cgacagcaaa aatgcgacag
     1081 cgcctacagg acccaatccc ggcaacacgc cgaccagagt accgaccacg gagccgagaa
     1141 aacaaaagag caaatttgtc ggtgtcagcg cgactgagaa gcccagcgcg acattggcga
     1201 aaatatccac attaaatacc caggattccg ccgggaagag gaatgcccag aatccaaccg
     1261 aagattgcgt aagaagcgat ggcggtcaaa accgcgacga aaaaaacttt ggaccagcgc
     1321 tgagattcga ccgctcgaag caggagaatc atcaggaaga acgtcgtgag taaaaacccc
     1381 aaccgctcga gcaccagggc aaagccgatc attgctgcca aggtgaatgc gatcctggac
     1441 caaccttccg gcaaaatatt atccggccga cggtccgact ccgcgccgaa gaatccctgt
     1501 gcgaacaaga ttacgcaaag aatcaaaagc agtgttgcga gtatccatgg gaaaaatccc
     1561 gtctgcggta ctcgcatagt gccaaacgaa agatgccggc tttctaataa gacgaacccc
     1621 gccaatccgg cgagaacagt cgagacagcg agttgggagc gcttcatcgc gccaaagcag
     1681 tctccgtgaa tcgctcaact gccgattttt gcaacgctcg cgacctccga ccacttgcgc
     1741 tcttcttcga ggtaaaactt cgctgcttct tgggggccga gattttcgac gagcgcttgt
     1801 gctttttcca cttgcccaat gatctccgga tctctgaacg tgtcgctgag cgcggcggcg
     1861 agtcgcttga cgatggcgcc ggaggtcttc gcgggcgcaa agaagccgat ccaaattttc
     1921 aagttcaact cgggaaatcc tttttcgacc gtggtcggca cattgggaaa atctttaagc
     1981 cggcgattcg aagcaacggc aagagcacgc atcgttcccg cttcaatgtg gcttcgagtt
     2041 gttccgagcg tcagaaaagc gatgttcaca tggccgccaa gcagggccgt tgcggcagcc
     2101 gattcagaac cgagcggcac atgcgtcaaa tcgatcttgg tttttagctt gaccaactcg
     2161 ccgataaagt gcggcgtcgt gccgggaccg gcggagccat aggtcagcgc gcccggattt
     2221 tttttcgcct cggctatgat atcttccagg gttttccacg gcgcatcggc tttgaccacc
     2281 gtggtgttag ggctgctggt cgccaaaacc agcggcgtga aatccttcat ggtgaaacct
     2341 gaattcttgt tcaccagcgg aatcagtagg attggcggtg gcgtgatcag aatcgtgtaa
     2401 ccatcgggcg cggcgtctct cgccgcttga attcccactg cgccaccgcc gccgg
//
LOCUS       DASULZ010000863         2382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1520320, whole genome shotgun sequence.
ACCESSION   DASULZ010000863 DASULZ010000000
VERSION     DASULZ010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520320"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VFY96_17760"
     CDS             <1..541
                     /locus_tag="VFY96_17760"
                     /inference="COORDINATES: protein motif:HMM:NF044544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608372.1"
                     /translation="KLLLFVGDRSMISVQDVENVMIDHGEAWIFDLTRALGDRDATAA
                     LSHLARLLAQGEAALKILATLTSETRRLLRARQLLATELAKLWRRGMSYQQFQNVVLK
                     DHGPLLTRNPYADYMCFQRAERFSLTELESYMEALFAADYRLKSGGTQPALVLERLVL
                     GLCMRRKQAAGLAARPVYR"
     gene            538..1080
                     /locus_tag="VFY96_17765"
     CDS             538..1080
                     /locus_tag="VFY96_17765"
                     /inference="COORDINATES: protein motif:HMM:NF019509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEX5608373.1"
                     /translation="MRKSIFSACLWVFYAVLTPSAASAEMIVQACFSPNGKCAAHVLR
                     EIGSAKKELLVAVYAFTSDDLANALVQAKKRGVSVQIVIDREFDLGNPSSKGKFLDAQ
                     KIPVRRISGAKPIGAEKDGGLMHQKFAIIDRSVVLAGSYNWTYAAENSNDENLLLFRE
                     AAPLAEEYRRAFLRLWERRP"
     gene            1077..1607
                     /locus_tag="VFY96_17770"
     CDS             1077..1607
                     /locus_tag="VFY96_17770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608374.1"
                     /translation="MTRFSSAVTSALLLSFLGGLFTHVLYQRWNPAAPEPFAVVSEQP
                     SVPGNAEPALHRVSFIPLAQPPEQKTVELPVLAARDVEKIRALADRQARVRGRIFRVG
                     HATKSNTYFLSFGPSREALTAVIFNSAAGLFEKSEQPPKNFENREVEISGFIKDHPQY
                     GLEIILEDPKQIKILN"
     gene            1619..1798
                     /locus_tag="VFY96_17775"
     CDS             1619..1798
                     /locus_tag="VFY96_17775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608375.1"
                     /translation="MSQSVKKCMVCAKPAENLRGGICEVCQDKIRREAMGEQARNSES
                     AERELSRHGVTPDKK"
     gene            2168..>2382
                     /locus_tag="VFY96_17780"
     CDS             2168..>2382
                     /locus_tag="VFY96_17780"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEX5608376.1"
                     /translation="MDAATGKVIYQKEADLRLPPASTTKIMTAILTLESGRSLLEKFT
                     VSKAATRVPASKLYLRPGQSLTIEELL"
BASE COUNT          584 a          628 c          656 g          514 t
ORIGIN      
        1 aaaattactt ctgttcgtag gtgatcgctc gatgatttca gtacaagacg tggaaaacgt
       61 catgatcgat cacggcgaag cctggatctt tgatctgacg cgtgcgcttg gcgatcgcga
      121 tgcgacggca gcgctttcgc acttggcacg tttgctcgct cagggagaag ccgctctcaa
      181 aatcctggcc acccttacga gtgagacacg gcgtttgctt cgagcgcgcc agctcttggc
      241 gacggaactc gccaagctgt ggagacgcgg catgagctat cagcagtttc aaaacgtagt
      301 cttaaaggac cacgggccgc tgcttacacg caacccatac gccgactaca tgtgctttca
      361 acgcgcggag cggttttccc tcaccgaact ggaatcttac atggaggcgc tcttcgccgc
      421 tgactaccgt ctgaaatcgg gcgggactca gcccgctctg gtgctcgagc ggctggtttt
      481 agggctctgc atgcgcagga agcaagcagc agggcttgcc gctcgtccgg tgtaccgatg
      541 agaaagtcga ttttttcagc ctgcctgtgg gttttctatg cggtactgac gccgagtgcg
      601 gcgagcgccg aaatgatcgt ccaagcttgc tttagtccca acgggaaatg cgccgcacac
      661 gttctgcgcg agatcggctc cgccaaaaag gaattgctcg tcgccgtgta tgcatttacg
      721 agtgatgatt tggccaatgc actggtgcag gcgaaaaaaa ggggagtttc ggttcagatc
      781 gtcatcgacc gcgagtttga tcttggcaat ccaagctcca aaggaaaatt cttggacgcg
      841 cagaagattc cggtacgccg gatttcggga gccaagccca tcggcgctga aaaagatggc
      901 ggtttaatgc atcaaaagtt tgcaatcatc gatcggagcg tagttcttgc cggctcctat
      961 aattggacgt atgcggcgga aaactccaac gacgaaaacc tgttgctttt tcgcgaagcg
     1021 gctcccctgg ctgaagaata tcggcgcgcc tttttgcggc tttgggagcg aaggccatga
     1081 cgcgattctc aagcgcagta acgagcgcat tgttgctgtc attcctgggc gggcttttca
     1141 cccatgtgct ctaccagaga tggaacccgg cggcgccgga gccatttgcc gtagtcagtg
     1201 aacagccttc ggtgccgggc aacgccgaac cggcgctgca ccgcgtgagt tttataccgc
     1261 tggcgcagcc gccggagcaa aaaaccgttg aactacccgt gctcgcggca cgcgacgtgg
     1321 aaaagattcg cgcactcgcc gatcgccaag cacgcgtccg cggccggatt tttcgtgttg
     1381 gccatgcgac caagagcaat acctattttt taagcttcgg gccatcacgc gaagcattga
     1441 ccgcggtgat cttcaattcc gcagcgggac tgtttgaaaa aagcgaacaa ccgccaaaaa
     1501 actttgaaaa tcgagaggtg gaaatcagcg gattcatcaa agatcatccg cagtacgggc
     1561 tggaaatcat tctcgaggat cccaagcaga taaaaatttt aaactgacag gaaacgccat
     1621 gagtcaatcg gtaaaaaaat gtatggtctg tgccaagccg gcggagaatt tgcgcggcgg
     1681 aatctgtgaa gtttgtcagg acaagatccg ccgcgaagcg atgggcgagc aggcgcgaaa
     1741 cagcgaaagc gccgagcgcg aacttagccg gcacggcgtg accccggaca aaaaataacc
     1801 gcccgtagca gaaaacattg tgattacatc gggaattcgc ctcccggttt attacatttg
     1861 cgacctgaac ggagtcgaac cgagatacct tcagcaacag gcaaatatgg ctgaccagag
     1921 tagaagtcaa catcgaggga tgctcggaaa aaaagctgat tttatttaaa actcgtggta
     1981 aaatccggtt ttactcagta agaaaaccgc atccaactaa gtctacaggc ggaggtcagc
     2041 aacactgcgc gttcaagatt tcatcggtag gatttttgtt ctcgccattt tcttcattgc
     2101 cgtgcccgca aactcaagct gggcattttc cccggatgaa gacctaacgg cacgggccgc
     2161 agttttgatg gacgcggcga cgggaaaagt aatctaccaa aaagaagccg atctccgtct
     2221 cccgccggca agcacgacca agattatgac ggctattctg acgctggaaa gcggccgcag
     2281 ccttctagaa aagtttactg tgtcaaaggc agccactcgg gttcctgctt ccaagctcta
     2341 cctccgtccg ggtcaatcat tgacgatcga agagttgctt ta
//
LOCUS       DASULZ010000864         4170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_810017, whole genome shotgun sequence.
ACCESSION   DASULZ010000864 DASULZ010000000
VERSION     DASULZ010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810017"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1174
                     /locus_tag="VFY96_17785"
     CDS             185..1174
                     /locus_tag="VFY96_17785"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5608377.1"
                     /translation="MARLLFLSVIFVLFAARATAASLEDAAKNEGEVVLYSSLNNEQI
                     VTLVEAFKKKHPFIKPSFYRGTSERVLQRASTEAKAGRFAVDVATAAGFQLQLMKESG
                     LTQKYVPPEAAFYNDGFKDPDGHWVSVHSLLNSMAYNTQLLKPNEAPKKYDDLLAPRW
                     KGRLGVNLQDPEWYVSLQRRWGKEKARNFLKALAAQQPGLRDGHNITAQLLAAGEFFA
                     VTNTYAHIAARIKNQGGPVQYVFDEPVITYVHPIALMKSAPHPNAAKLLIGFILSTEG
                     QRMLREQGRIPSHRDIDPLVFSLRNVKLFASDPKFAKEYAPAGEEMRAIFGIR"
     gene            1238..3127
                     /locus_tag="VFY96_17790"
     CDS             1238..3127
                     /locus_tag="VFY96_17790"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5608378.1"
                     /translation="MTELFPKTRTTALPGGYMGKILRVDLTRGKLKDENLPEEPILKK
                     FIGGQSLALYILLGELPLDATPFGPENSVVMMTGPLTGNGFTPGGTKMTAVYLSPLTV
                     NTVGRGATSGYWGTYLKAAGYDGIILNGAAPNPVYLYIKDGRAELRDASHVWGLGTRD
                     TEDRVRELVRDRDARVLCIGPAGENLNRAAMLANDYNHFAAHSGGAVLGAKKLKAIVV
                     SGTLRPSYLDKKRLVDAGARWRAVLQQHDINKKKTKYGHGDAWGALNNLNWRSTDLQP
                     AHIKGFDQNEVVLRPCFQCARMCPWDAKLGEGEFKGTVVHFNAGGEWLDTFFNLGIKG
                     NAVLYLAEKINNLGIECSHFSCGAGVAFEAWEKGLLDPARTNGLQLEWGNVEAVDKLL
                     DMAAHREGWLGNLLAEGPKQLAEALGGDAPKWAVHTKGGTPAMHEWRPLLGQMLRELV
                     ASGGMKPQGGGSKNPPPDLRDTEKWGPLEMDNPEGWVWSHVLSEQYRQFTGIFGGCWF
                     AQESHKKDGLNSIVDAFNAITGWNFSMDDALRAGHRSMILQSLFATQRGWIADFDWQD
                     VGPRFLEPIPDGKYKGFTIAKWLPDMIWEYYKLTGRHERTGRPFKDTLEKLGLEEFSQ
                     WAQLD"
     gene            complement(3323..4051)
                     /locus_tag="VFY96_17795"
     CDS             complement(3323..4051)
                     /locus_tag="VFY96_17795"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608379.1"
                     /translation="MSSGTWRGTVGVVKPTYGSGSLVEFIRLLPEGIGCIPMYAGIKE
                     HSERGYLGALETYHAKVAELADIGVDLIHPEGGPPFMVRGHKAEQEIVAGWEQTYNIP
                     IFTSGISQAEALRALGIKRFVGCTYFNDKKLNDLFARYFSDAGFEVLGMEGMNTTPYE
                     ADKISTDTIYQHLKTSFNKFPNAQGIYLLGSGAWQVKDIVAVENDLGVPVVHPVAARV
                     WLVQKKLQVRNPIRGASRLLEELP"
BASE COUNT          913 a         1196 c         1174 g          887 t
ORIGIN      
        1 acgaaatcgc tgggtacgcg agcgagcgcg aagttctcgg cgaggatcct tacgcctatg
       61 tcatgggcga aaacgaaaag cgcactctgg ctgcgatgaa ccgatatcag atcgagcagg
      121 gcttgatgaa aacgaccttg ccgatggaag atctcttcgt cggagatatg cgaggttgag
      181 ccaagtggcc cgactgttat ttctctccgt gatcttcgtg ttattcgccg cgcgcgcgac
      241 ggctgcctct ctggaagacg ccgccaaaaa cgagggcgaa gtggtgcttt acagctcgct
      301 caataacgag cagatcgtta cgctggtcga ggcattcaag aaaaaacatc cgtttatcaa
      361 gccatcgttc tatcgtggca caagcgagcg cgttctgcag cgagcctcga ccgaagccaa
      421 agccggccgg ttcgccgtcg acgtcgcgac cgcggcggga tttcagctcc agctcatgaa
      481 agaatctggg ctgacccaga aatacgtgcc gccggaggcc gctttctaca acgacggctt
      541 caaggatccg gacgggcatt gggtgagcgt ccactcgttg ctcaattcga tggcctacaa
      601 cacccaactc ttaaaaccca acgaagcgcc caaaaagtac gatgacctgc tcgccccccg
      661 ctggaaaggc cggctcggtg tcaatctgca ggatcccgaa tggtacgtga gcctgcaacg
      721 tcggtggggc aaagagaagg cgcgtaattt tctcaaagct ctggcagccc agcagcccgg
      781 tctgcgcgat ggccacaaca tcaccgcgca attacttgcc gccggcgaat ttttcgcagt
      841 caccaatacc tacgcgcaca ttgccgcgcg catcaagaat cagggcgggc cggtgcaata
      901 tgttttcgac gagccggtga tcacctatgt tcatccaatt gcgttgatga aatccgcgcc
      961 gcatccgaac gccgcgaagc tattgatcgg ctttattctt tccaccgaag gtcaacgcat
     1021 gctgcgcgag cagggcagaa ttcccagtca ccgcgacatc gacccgctgg tattttcctt
     1081 gcgcaacgtg aaactctttg ccagcgatcc taaattcgcc aaggaatacg ctccggcggg
     1141 cgaagagatg cgcgccatct ttggcattcg ttgattttgg attggctttc agcgatttga
     1201 acggcttgaa cgagctcgat gttgagcagc cgccacgatg actgaattat tcccgaaaac
     1261 ccgcaccacc gccctccccg gcggctacat gggcaagatt ctgcgcgtcg atttgacccg
     1321 cggaaaattg aaggacgaga accttcctga agaaccgatt ttgaagaagt tcatcggcgg
     1381 tcaatcgctg gcgctttata ttttattggg cgagttgccg ctcgatgcga cgccgttcgg
     1441 gccggagaac agcgtcgtca tgatgaccgg gccgctcacc ggcaacggat ttactcccgg
     1501 cggcaccaaa atgaccgccg tttatttgag cccgctcacc gtcaacacgg tgggtcgcgg
     1561 cgcgaccagc ggctattggg gaacttacct caaagcggct ggttacgatg gcatcatcct
     1621 caacggcgct gcgccaaatc ccgtttatct ttacatcaaa gatggccgcg ccgagttgcg
     1681 cgacgcttcg cacgtctggg ggctcggcac tcgcgacacg gaagatcgtg tgcgcgagct
     1741 cgtccgcgat cgcgacgccc gggtattgtg tatcggcccc gcgggggaaa atctcaaccg
     1801 cgcggcaatg ctggcaaacg actacaatca ttttgccgcc cacagcggcg gtgcggtgct
     1861 cggcgcgaaa aagctcaaag cgatcgtcgt ttcgggtacg ctgcgaccga gttacctgga
     1921 caaaaagcga ttggtcgatg ccggcgcgcg ctggcgcgcg gtgttgcagc aacacgacat
     1981 caacaaaaag aaaaccaaat acggccatgg cgacgcctgg ggcgcactaa ataatctcaa
     2041 ttggcgcagc accgatctac aaccggcgca tatcaaaggc ttcgatcaaa acgaggtggt
     2101 gctccgcccc tgttttcagt gcgcccggat gtgcccatgg gacgccaagc tcggcgaagg
     2161 tgagttcaaa ggcacggtgg ttcatttcaa cgccggcggt gaatggctcg ataccttttt
     2221 caatctcgga atcaaaggca acgcggtgct ctacttggcg gagaagatca ataatttggg
     2281 cattgagtgc agccattttt cctgcggcgc cggagtcgcg ttcgaagctt gggagaaggg
     2341 cctgctcgac cccgctcgca ccaatggctt acagctcgaa tggggcaacg tcgaggctgt
     2401 cgacaagctc ttggacatgg cggcccatcg cgaaggctgg ctcggcaact tgctcgccga
     2461 aggaccgaag caactcgccg aagcgctcgg cggtgatgcg ccgaaatggg cggtgcatac
     2521 gaaaggcggc acaccggcga tgcatgagtg gcgcccgctg ctcgggcaga tgctgcgcga
     2581 gctggttgcg agcggcggta tgaagccgca gggcggcggc tcgaagaacc cgccgccgga
     2641 tctccgcgac acggaaaagt ggggcccgtt agaaatggac aatccggaag gctgggtttg
     2701 gtctcacgtg ctttcggaac agtatcggca attcaccggc atcttcggcg gctgctggtt
     2761 tgcgcaggaa agccacaaaa aagacggact caacagcatc gtcgatgcct tcaatgcgat
     2821 taccggctgg aatttttcta tggacgacgc gcttcgagcc ggccatcgga gcatgattct
     2881 ccaaagtctc ttcgctactc aacgcggctg gatcgccgac ttcgattggc aagacgtcgg
     2941 tccacgattt ttggaaccga tcccagatgg caaatacaaa ggcttcacga tcgccaaatg
     3001 gttgccggat atgatctggg agtattacaa gttaaccggc cggcacgagc gcaccggaag
     3061 gccattcaaa gatactctgg aaaaactcgg cctggaagaa ttctcgcagt gggcgcaact
     3121 cgattgagcc cagagtaggg tgtcatgtgt gatactgctc gttcctcagg tgtcattctg
     3181 agtgtcagcg aagaatctcg ctttcctgct cctaacggtg cgagatcctt cggctgagtt
     3241 tatcctgagt aaaatcgaag ggcctcaggg tgacactgca gaacaatgcc gtcgaggcag
     3301 ctgatgctat cattccttga tctcacggca gctcttccag cagcctactt gcgcccctga
     3361 tcgggttcct gacctgcaat ttcttctgca ctagccagac ccgcgcggcc acgggatgca
     3421 cgacgggaac tccgaggtcg ttctccaccg cgacgatgtc cttcacttgc caagcgcccg
     3481 agccgagcaa atagattcct tgtgcattag gaaacttatt gaacgaggtt ttcaggtgct
     3541 gatagatcgt gtccgtggaa attttatccg cctcgtacgg agtcgtgttc atgccctcca
     3601 tgccgagcac ttcaaaaccc gcatcgctga aatagcgcgc gaacaaatcg ttgagttttt
     3661 tgtcgttgaa gtaagtgcag cccacgaatc ttttgatgcc gagcgcgcgc agcgcttcgg
     3721 cctgagaaat tccggaagtg aagatcggta tgttgtatgt ctgttcccaa ccggcgacga
     3781 tctcctgctc ggctttatgg ccgcgcacca tgaacggcgg cccgccttcg ggatggatca
     3841 gatcgacgcc gatgtcggca agctccgcga ctttggcgtg ataggtttct agcgcgccga
     3901 gataaccccg ttcggaatgt tctttgatgc ccgcgtacat cggaatgcag ccgatgccct
     3961 ccggcagaag ccgaatgaat tccaccagtg accctgaacc ataagtgggt tttactaccc
     4021 caaccgttcc gcgccaggtt cctgaagaca tgatcgactc ctcttacttg ttgtcttcgc
     4081 ccttctctgc cttgtccctg agccctatac cggctgaagg attactttga tcgctgttca
     4141 ccgttcattc tttcttgtta tcctcccact
//
LOCUS       DASULZ010000865         2660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1520607, whole genome shotgun sequence.
ACCESSION   DASULZ010000865 DASULZ010000000
VERSION     DASULZ010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520607"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VFY96_17800"
     CDS             <1..578
                     /locus_tag="VFY96_17800"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX5608380.1"
                     /translation="KTAEALSAHKVGAIGAETAKRLEAARIRASLVPARYQAEGILDA
                     ISPEEIGGKRVLIPRAAEAREILPATLRKWGALVDVIVAYRTELPRLDVRPLADLLVE
                     RKVDVITFTSSSTVRNFVRLFGGRKLGEIVSGSTIACIGPITAATVEELGARADIVAD
                     QFTVTDMLRAIVEHFQANSKANRRRGSEVIE"
     gene            714..1688
                     /gene="hemB"
                     /locus_tag="VFY96_17805"
     CDS             714..1688
                     /gene="hemB"
                     /locus_tag="VFY96_17805"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX5608381.1"
                     /translation="MNFPIYRPRRLRRNERFRNLIRETTLQPKNLIYPLFIGPGRDKA
                     EPVSSMPGIAQLSVDRAIREAEEVNSLGISAVILFGIPKQKDAFGSEAYAADGVVQQA
                     IYAIKEKIPELLVITDVCLCEYTDHGHCGVVKNGEIDNDSTLELLAKEALSHAEAGAD
                     IVAPSDMMDGRVAAIRKILDDHNFQQTPIMAYAAKYASGFYGPFREAAESTPQFGDRR
                     SYQMDPANAEEALREVDLDIREGADIVMVKPALAYLDIIYRVKEKFGYPLAAYNVSGE
                     YSMIKAAGQNGWIEESRIMMEVLFAIRRAGADMILSYFAKDAARLLDN"
     gene            complement(1702..1962)
                     /locus_tag="VFY96_17810"
     CDS             complement(1702..1962)
                     /locus_tag="VFY96_17810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608382.1"
                     /translation="MLSSQRGDAAAADIQRWASAIAKRIDSEIGITVEFDADSKTFIV
                     RLIKSSRVLIFRFSESQVHTDGREVECERTLNRKIKDLWNLL"
     gene            complement(1986..>2660)
                     /locus_tag="VFY96_17815"
     CDS             complement(1986..>2660)
                     /locus_tag="VFY96_17815"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5608383.1"
                     /translation="YVLMGKPGIKSAEQLKGGTVAISRFGSATDTIARFALRKIGLNP
                     GKDVTLVQVGSGPERLSAALTGRVTASVINPPSSFIAEKKGLAIIADVAKMGLVFQHT
                     GAATTRKYIKEHQETVRRYVRAHVEAVHKMWTDKEATIKALSHYMGSGLDRETLEKSY
                     DNVMTEAMYPKKQYPSLEGLKTVLDDLAERDPRAKNAKPEQFVDMTFIRELDQSGFID
                     ALYKKK"
BASE COUNT          593 a          711 c          753 g          603 t
ORIGIN      
        1 gaaaaaccgc tgaagcgctg agcgcgcaca aagtcggcgc gatcggcgcg gaaacggcaa
       61 aacgactgga agctgcacgg attcgcgcat ctctcgtccc ggcgcgctac caggccgaag
      121 ggattttgga tgcgatctca cctgaagaaa tcggcggcaa acgagtgctg atcccccgag
      181 cggccgaagc acgggaaatt ctgccggcta ctttgcgaaa atggggcgcg ctggtggatg
      241 tgatcgtggc ctatcgcacc gagctgccgc gccttgatgt ccgcccactt gcggacttgc
      301 tcgtcgagcg caaagtcgat gtcattacct tcacaagctc gagcacggtg cggaattttg
      361 tccggctttt cggcggtagg aagctcggcg aaattgtcag cggaagcacg atcgcctgta
      421 tcggcccgat tacggcggca accgtcgagg agctaggcgc ccgcgccgat atcgttgcgg
      481 atcagtttac ggtcacagac atgttgcgcg ccatcgttga gcattttcag gccaattcga
      541 aagcgaaccg tcgaaggggt tctgaagtta ttgagtgaac attccgtcat tacggtcttc
      601 ggcaatgctc agcataaact ccagccggaa gccaggattt tgaacttgat cgataaccaa
      661 tctgtaggcg ccggcatcac aacattctaa cttcacttgg cttggagcgg atcatgaatt
      721 tcccaatcta ccggccgaga aggctacggc gtaacgaaag attcagaaat ttgatcagag
      781 aaacgacgct gcagcccaaa aatctgatct atccactgtt cattgggccg ggcagagata
      841 aagccgagcc ggtatcgtcc atgccgggca ttgcacagtt gagcgtcgat cgagcgattc
      901 gcgaagcaga ggaggtcaac tcgctgggca tctccgcggt gattctattc ggtatcccaa
      961 aacagaagga tgccttcggc agtgaagcct acgctgcgga cggagtcgtc cagcaggcaa
     1021 tttacgcgat caaagaaaaa attccggagc tgctcgtaat tactgacgtt tgcctttgcg
     1081 agtataccga ccatggtcat tgcggcgtgg tgaaaaacgg cgaaatcgat aacgacagca
     1141 cgttggagtt gctggccaag gaggcattat cccatgcaga agcgggcgct gatattgttg
     1201 cgccatcgga catgatggac ggacgggtcg cggcgattcg taaaatactc gacgatcaca
     1261 attttcagca gaccccgatc atggcgtacg cggccaaata tgcttcgggc ttttatggtc
     1321 ccttcaggga ggccgcggaa tcaacgccgc aattcggcga tcgtcgctct tatcagatgg
     1381 acccggccaa cgccgaggag gcattgcgcg aagtcgatct cgacattcgc gagggcgcgg
     1441 atattgtcat ggtcaagccg gcgttggctt accttgacat catctaccgg gtcaaagaga
     1501 agttcggtta tccgctggcg gcctacaatg tgagtggtga atattcaatg atcaaggcgg
     1561 cgggccagaa cggctggatt gaagagagcc ggatcatgat ggaagtcttg tttgcgatcc
     1621 gccgggcggg cgccgacatg atcctttcct acttcgccaa ggatgcggcg cgactgctgg
     1681 acaactaacc attcccggtt tctaaagcaa attccacaaa tctttaatct tccggttcag
     1741 cgtgcgctcg cattccacct cccggccgtc ggtgtgcacc tgtgattcgg aaaaccgaaa
     1801 aatcagaacc ctggaggatt tgatcagccg aacgatgaag gtcttggaat cggcatcgaa
     1861 ctcgaccgta atgccgatct cagaatcgat gcgcttggcg attgcgctgg cccagcgctg
     1921 gatgtcggcg gcagccgcat cgcctcgttg cgagctcaac atgaagcgag gggacgagcg
     1981 aagggttatt tctttttgta aagtgcgtcg ataaaaccgc tttgatcgag ctcccgaatg
     2041 aaggtcatat cgacgaattg ctcaggtttg gcgttcttgg ctctgggatc tcgctcggcg
     2101 agatcatcga gaacggtttt gagtccttcc aggctgggat actgcttttt cgggtacatg
     2161 gcttccgtca tcacgttatc gtagcttttc tccaaggttt cccgatcgag accgctcccc
     2221 atatagtggc ttagcgcctt gatcgtagcc tctttgtcgg tccacatctt gtgcaccgcc
     2281 tccacgtgcg ctcggacata gcggcgcacc gtctcttgat gttctttgat atatttccga
     2341 gtggtggctg caccggtgtg ttgaaagacc aagcccattt ttgctacgtc ggctatgatc
     2401 gccaaacctt tcttttcggc aatgaagctg gagggcggat tgatgaccga tgcggtgact
     2461 cgacccgtca aagccgcgct cagccgctca ggaccgcttc ccacttgcac gagagtgaca
     2521 tcctttccgg gattcaagcc gattttccgc agcgcaaatc gagcaatggt atcagtggca
     2581 gagccaaatc ggctgatcgc taccgtaccc cctttgagtt gctcagcgct tttaattccg
     2641 ggcttcccca tgaggacgta
//
LOCUS       DASULZ010000866         6057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_403344, whole genome shotgun sequence.
ACCESSION   DASULZ010000866 DASULZ010000000
VERSION     DASULZ010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6057
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403344"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="VFY96_17820"
     CDS             complement(<1..1010)
                     /locus_tag="VFY96_17820"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5608384.1"
                     /translation="MQPRGGHVHLSLFSRLTIGYLVIFLVVATASASAVLQLRYFRDL
                     TESILNIDNRMLDHEKILAELLLAQSRAEQKFIITRDETWYQQFVRLKSEFDSRLQSA
                     LALNDPAAASILKAIQRDSERYQQLVQDEGHLVRANKRYPQAQYKSDKDVLVDALLSA
                     CEKLRQNQQRVTHAKVADLAAAADQAGETAVIIAASCLLAIITMSLLITKSITRPIEL
                     LKTRTRDIAEGNFAGSVPVRSPPEIAELAVAIDRMSEKLHELDRLKADFFASMSHELR
                     TPLTSIKEGTGLLLDGVGGETSEKQRKLLSILAEESNRLISVVNSLLDLSKLEAGMMA
                     YD"
     gene            complement(1011..2141)
                     /locus_tag="VFY96_17825"
     CDS             complement(1011..2141)
                     /locus_tag="VFY96_17825"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX5608385.1"
                     /translation="MELSTFLLFFLLVIFALTFLGWLIISRLKNLFEQSRDQQSMLLM
                     QQQIGELRAQLSNALDHGAQSIQQQLGQMIGHVNERLRENAEVLNRAQQSLGERLDNA
                     ARVVGHVQRSLGGLEEANRKIYEVGKDIASLHEILRAPKLRGGLGEFFLEDLLGQILP
                     PDHFATQHCFRTGDKVDAVVKLGASLVPVDAKFPLENFKRMMEAASDEEKTRAKKQFI
                     ADVKKHIDAIASKYILPDEGTYDFALMYIAAENVYYETIIRDEAPAEKSLCHYAMAQR
                     VIPVSPNSFYAYLQAIVLGLKGMKIEDRAKEIIQYLGRLQGDFTRFRDEFAVLGKHIG
                     HAQASYLAADRRLEQFGQKFIVADGNQPELFDSDGLPERNKS"
     gene            complement(2159..2371)
                     /locus_tag="VFY96_17830"
     CDS             complement(2159..2371)
                     /locus_tag="VFY96_17830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608386.1"
                     /translation="MGRVIQLNELHQARRRRSEKVSMEQCVELLEWNLKKSVDDYFAA
                     PREERSMRATQIRKLSEILEYAVRLL"
     gene            complement(2454..3536)
                     /locus_tag="VFY96_17835"
     CDS             complement(2454..3536)
                     /locus_tag="VFY96_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEX5608387.1"
                     /translation="MSGQSSTVLDDKRATPRSLGYSMPAEWTAHSATWLSWPHNLETW
                     PTQLESVREVWIQMISLLAPHERVYVLVNDQRAENEVYARLESARAATENVRLLKIPT
                     IDVWMRDYGPTFVTRSAPENPLAFNDWLFNGWGGKYPSYELDDRVARELALFLKVPVF
                     KHEIVLEGGSIELNGAGTCLTTEQCLLNQNRNPQLSRAEIDGFLKDSLGVSHMIWLGD
                     GIVGDDTDGHIDDIARFIDAKTVACIVEENSRDENYRFLQENYERLQNAVDQNGARFS
                     VVRLPCPEPVYDRGVRLPASYANFYIANEVVLVPIFNDPRDVGALGLLQELFPNRKVH
                     GLRCNDVVAGLGAIHCVTQQQPWTPL"
     gene            complement(3533..4429)
                     /locus_tag="VFY96_17840"
     CDS             complement(3533..4429)
                     /locus_tag="VFY96_17840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HEX5608388.1"
                     /translation="MARRAKKEVVRLGLIQMSAGENPEDNLAKAMERIAAAANQGAQI
                     VCLQELFRSRYFCQSANQRNFKFAEPIPGPTTDALSALAAARQIVVVASVFERRSAGI
                     YHNTAVIIDADGSIAGIYRKMHIPDDPLYYEKFYFTPGDLGFPSFQTRYAKVGALVCW
                     DQWFPEGARSAALSGAQILIYPTAIGWIPNEPKLIAGQQRDAWELIQRSHAVANGVYV
                     ASVNRVGREGRIKFWGHSFVAGPFGEIIAHAGGEREEILLGNCDLAKTEEIRQSWPFL
                     RDRRIDAYGPLLSRSLEDEKRR"
     gene            complement(4514..5233)
                     /locus_tag="VFY96_17845"
     CDS             complement(4514..5233)
                     /locus_tag="VFY96_17845"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5608389.1"
                     /translation="MDVTSSTVQLNTQDGKMDAYVAQPKESGNYPGIVVIQEAFGVNS
                     HIKNVTERIAAEGYVAIAPDIFHRESERLIPYSDMPKAIATLQRVVDSKAMEDVGAAI
                     AHLKSQSNVKSDAIGVIGFCMGGRLTYLAAAHHANDVKAAAPYYGGGITMGNPSPLSR
                     TGGIKCPMYLFFGAKDQLIPMDQVGQINTELTTKKVPFQMKIYPEAGHGFFCDERGSY
                     HEASAKDAWEKSKSFFAQHLK"
BASE COUNT         1343 a         1685 c         1651 g         1378 t
ORIGIN      
        1 aaatcataag ccatcatacc cgcttcaagc ttagaaagat ccagcagcga attgaccacg
       61 ctaataagac gattgctttc ctcggccagg atgctgagca gcttgcgttg tttttccgaa
      121 gtctcgccgc cgaccccgtc gagtaataat ccggtgccct ctttgatcga cgtcaacggc
      181 gttcgtagct cgtgcgacat cgaagcaaag aagtccgcct tcaggcgatc gagctcatgc
      241 agcttctcgg acatccggtc gatcgccaca gccagctcag cgatctccgg cggtgaacgg
      301 accggcacgc taccggcgaa attgccctcc gcgatgtcgc gcgtcctcgt cttcagcagc
      361 tcgatcggcc gggtgatgct tttggtgatg agcaacgaca tcgtgatgat tgccagcaag
      421 cagctcgccg caatgatcac cgctgtttca ccggcttggt cggcagcggc ggcgagatcg
      481 gccactttcg cgtgcgtcac tcgctgctga ttctggcgca gcttttcaca cgccgagaga
      541 agcgcgtcga cgagaacatc tttatccgat ttgtattgtg cctgcggata acgcttgttg
      601 gctcgcacca gatgcccttc gtcctgcacc aactgctgat atcgctcaga gtcgcgctga
      661 atggctttga ggatcgaagc ggcagccgga tcgttcaaag ccagggcgct ttgcaagcgc
      721 gagtcgaatt cagatttgag ccggacaaat tgctggtacc aggtctcatc gcgggtgatg
      781 ataaatttct gttcggcccg actttgcgcc aataaaagct ccgcgaggat tttctcgtgg
      841 tcaagcatgc ggttatcgat attgagaatc gattccgtga gatcgcggaa atatctgagc
      901 tggagaaccg ccgacgcgct cgccgtcgcg accaccaaga agatcacgag atagccgatc
      961 gtcaggcggg aaaacagact caggtgcacg tgaccgcccc tcggctgcac tcaactcttg
     1021 ttgcgttcag gtaggccatc ggaatcgaag agctctggtt gattgccgtc ggcgacgatg
     1081 aacttctgac cgaattgctc caaacgtcgg tccgccgcga gatagctcgc ctgagcgtgt
     1141 ccgatatgtt taccgagaac ggcaaactca tcacgaaatc tcgtgaagtc accttggagc
     1201 cggccgagat actggatgat ttccttcgcg cggtcttcga ttttcatgcc ctttaagcca
     1261 aggacgatcg cctgaagata ggcgtaaaaa ctattgggcg acacgggaat gacgcgctgc
     1321 gccatggcat agtggcagag gcttttttcc gccggcgcct cgtcgcggat aatcgtctcg
     1381 tagtagacgt tttccgcggc tatatacatc aacgcgaaat cgtaggtgcc ttcgtcgggc
     1441 aagatgtact tggacgcaat cgcatcgatg tgtttcttga catcggcgat gaactgtttt
     1501 tttgcccggg ttttttcttc atcgctcgcg gcttccatca tccgtttgaa gttttccaga
     1561 gggaacttgg catccaccgg gacgagcgat gcgccgagct taaccaccgc atcgacttta
     1621 tcgccggtac gaaaacagtg ttgtgtcgca aaatggtcgg gcggcagaat ctgtccgagt
     1681 aggtcctcga gaaaaaactc accgagcccg ccgcgaagct ttggcgctcg tagaatttcg
     1741 tggagtgacg cgatatcctt gccgacttcg tagatttttc ggttcgcttc ctcgaggccg
     1801 cccagacttc gttgcacatg gccgacgacg cgcgcggcat tgtccagcct ttcgcccaaa
     1861 ctttgttgcg ctctgttcaa aacttccgcg ttttcccgca aacgttcgtt cacgtgcccg
     1921 atcatttgcc ccaactgttg ctggatcgac tgagcgccat gatcaagggc attactcagc
     1981 tgcgcgcgca gctcgccaat ttgctgctgc atcagcagca tcgattgttg atcgcgactc
     2041 tgctcaaaga gatttttgag ccggctaata atcagccagc caagaaaagt cagcgcgaaa
     2101 ataaccagga gaaaaaacag caggaaggtg gacagttcca caatgaaccg tcgctcaatc
     2161 agaggagcct tacagcgtat tcgaggattt cactgagctt acgaatttgc gtcgcgcgca
     2221 tggagcgctc ttcacggggt gccgcaaaat agtcatcgac gctctttttc aaattccact
     2281 ccagcaattc cacacattgt tccatgctca ccttttccga acggcgccgg cgcgcctgat
     2341 gcagttcgtt gagctggatg acccgaccca taacacggct tagaatagga gagtgcgaag
     2401 aattgtcaag ggcttcaagc aagacctcgg cgaatgatgc ggaaccaaga tcatcacagc
     2461 ggcgtccacg gttgctgctg cgtaacgcaa tggatcgcgc ctaatccggc gacgacgtca
     2521 ttgcagcgca gtccgtgcac ttttctgttc gggaatagtt cctgtaaaag cccgagggcg
     2581 ccgacgtcgc gcgggtcatt gaaaatcggc accagcacga cctcgttggc gatatagaag
     2641 ttggcataac tcgcgggtaa gcgcacgccg cgatcataca cgggctcggg gcacggcaat
     2701 cttaccacgg agaatctggc gccgttttgg tccacagcat tttgcaggcg ctcgtaattt
     2761 tcttgcagaa agcgataatt ctcgtcccgc gagttctcct ccacgataca cgccacggtt
     2821 ttcgcgtcga tgaaacgagc gatgtcgtcg atgtggccgt ccgtgtcgtc gccgacgatt
     2881 ccgtcgccaa gccaaatcat gtgactgact cccagcgagt ctttgagaaa gccatcgatc
     2941 tctgcgcgac tcaattgcgg attgcggttc tggttcaaaa gacattgctc cgtcgtcaag
     3001 caggtgccgg caccgttgag ctcgatggag ccgccttcga gaacaatctc gtgtttaaaa
     3061 accggcactt tcagaaataa tgcaagttcg cgagcgacac gatcgtcgag ctcataggaa
     3121 gggtacttgc ctccccagcc gttaaacagc caatcattga aggcgagcgg attttccggc
     3181 gcgctgcgtg tcacgaacgt cggtccgtag tcgcgcatcc agacatcgat cgttggaatt
     3241 ttgaggagcc tcacattttc cgtggcagcg cgtgcgctct ccaggcgcgc ataaacctca
     3301 ttctccgccc gttggtcgtt gacgagaaca taaacgcgct cgtgcggcgc caggagtgag
     3361 atcatttgga tccagacctc gcgcaccgac tccagctgtg tcggccaggt ttccagattg
     3421 tgcggccagg agagccaggt ggcgctgtgc gccgtccact ccgccggcat cgaatagccg
     3481 agcgagcgcg gcgtcgcccg tttgtcatct aagacggtag aagattgacc gctcaacgac
     3541 gtttctcatc ctcgagggaa cgagacagca atggtccgta ggcatcgatg cggcggtccc
     3601 gcaggaatgg ccagctctgg cgaatttctt cggttttcgc taggtcgcag tttcccaaca
     3661 aaatttcttc ccgctcaccg ccggcatgcg caatgatctc accaaacggc cccgcgacaa
     3721 acgaatggcc ccaaaattta atccggcctt cacggccgac gcgattcacc gacgccacgt
     3781 agacgccgtt tgccaccgcg tgagagcgtt gaatcagctc ccatgcatca cgctgctgac
     3841 cggcaatcaa tttcggctca ttgggaatcc agccgatcgc cgtggggtag atgagaattt
     3901 gcgcccccga taacgccgcc gatcgagcgc cttcggggaa ccattggtcc caacagacga
     3961 gcgcgccgac tttggcgtaa cgcgtctgaa agctcggaaa cccgagatcg cccggggtga
     4021 agtaaaactt ttcatagtag agcggatcgt ccgggatgtg catcttgcga tagatgccgg
     4081 cgattgagcc atcggcgtcg atgatcactg cggtgttgtg atagatcccc gccgatcgtc
     4141 tttcaaacac cgaagccacg acgacgatct gtcgcgccgc tgcaagcgcg ctcaaagcat
     4201 cggtggtcgg tccagggatt ggctcggcga atttgaagtt gcgctggttt gcgctctggc
     4261 agaagtagcg cgaccggaaa agctcttgaa gacatacgat ctgcgcgcct tggttggcag
     4321 ccgcggcgat cctttccatc gctttggcga gattatcttc gggattctca cctgcgctca
     4381 tctggatgag ccccaagcga acgacttctt ttttagcccg acgcgccatg aacagagtat
     4441 ttacgctttt aaaataaaaa gggagaggaa agcgattttc ttccctcccc ctaaatggtt
     4501 cctcagtgcc ctgctatttc aagtgctgag caaaaaacga cttgctcttt tcccacgcgt
     4561 ccttggccga ggcttcgtgg tagctgccgc gctcatcgca aaaaaagccg tgcccggctt
     4621 cggggtaaat tttcatctga aagggaactt tcttggtggt taattcagtg ttgatttgac
     4681 cgacctgatc catgggaatg agctgatcct tcgcgccgaa aaacaaatac atcggacatt
     4741 tgattccgcc ggtccgggag agtgggctgg gattgcccat agtaatgcca ccgccgtaat
     4801 aaggcgcggc ggctttgaca tcgttggcgt gatgtgcggc cgcgagataa gtgagccgcc
     4861 cgcccatgca gaagccaatc acgccgatgg cgtcggattt gacgttgctt tgcgatttca
     4921 gatgggcgat cgccgcgccg acatcttcca tcgccttgct atcgacgacc cgctgcaatg
     4981 tggcgatcgc cttcggcata tcggaataag ggatcagccg ctccgattcg cggtgaaaga
     5041 tatcgggtgc aatggccacg taaccttcgg cggcaatgcg ctcggtaacg tttttgatgt
     5101 gactgttgac gccgaacgct tcctgaatga cgacaatccc tgggtagttc ccgctctcct
     5161 tcggctgcgc cacatacgca tccattttgc catcctgagt attgagttga accgtcgaac
     5221 tcgtcacgtc cataaacggc ctcctagagc tgatttttca tctaacgatg gaagaattca
     5281 ctatcagatt tctcattatc ggtccacagc cgcgatcgaa actgacgcac tgaatgatgt
     5341 gctgcgaact gcgacgaagt taactgctat tgtgcgcaac ggcaaaacgt ccgtgggatt
     5401 atgtttcgac agcagttcga gctcgaaggt tgccgcggca ggaaagagtc ggattgatgc
     5461 ggtacagatc tgactgaaga accgattcat cggctggtcg atcacggtca ggtttcaaga
     5521 acgctctcga aatcggcacg actggttatt ctgcggtcgc ccggcggcgt tgatctccac
     5581 agctgcctag gaaacggaaa aaaccgacgg cagatcgtgt ttgtgtgggt taaaccggca
     5641 gggacggtgt ctccttggga ttttaaatca aaaggtccac gtatttgttg agattccact
     5701 tttgttctac gcggcatggc aattgcgcct cttaagagca gcaaccagaa tcctcaacgc
     5761 ggaacatcac ctcaccgagt ttgttgtaac cgaccgctat cttcaacgac gtcttgccgc
     5821 acgacccgag gagcaatcag gcccggcaat tagcagcagt gatcaggtaa tttcgataag
     5881 aaaggaggtt cgtgaaaact gttcagctcg tatctgaatg tctcaagaac gatcggacca
     5941 ggaatgatca atcaaaccgt ttcggagcgg cggatagtgg cggcggaacg tccggatcgt
     6001 aacgacgcga cgagaacagg tctctcgacg aatggcaaac gtaagttcat acgtcga
//
LOCUS       DASULZ010000867         3163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1520732, whole genome shotgun sequence.
ACCESSION   DASULZ010000867 DASULZ010000000
VERSION     DASULZ010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520732"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VFY96_17850"
     CDS             complement(<1..302)
                     /locus_tag="VFY96_17850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608390.1"
                     /translation="MRKHRIIAAVFLTLGVAFFGARFSANAQDVKAKAEAEGKLMMYA
                     TFTAADSKTLVDAFKQVYPKIDAAYYRASDAALVERFVNESRAGQNICDVMLTTSF"
     gene            complement(329..1051)
                     /locus_tag="VFY96_17855"
     CDS             complement(329..1051)
                     /locus_tag="VFY96_17855"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608391.1"
                     /translation="MYYGARGIVGLVKPTYRPGSMESFIKLMPEGVGFIPAHAGIRAG
                     NEKELQEALTVAERKVARLAELGADVVMIMGAPPTMVRGHGADREIADSLSRKYKIPV
                     TVATIAQVDAFRALGMKRLVGITYFQDDMNRRFAKFLQDDGFDVKAMNGIEVPFKDAG
                     KLAPEAIYNFAKKTFLEHGPADGIYMLGAGWHNLPVIDMLEKDLKTTVLSSIPAQVWA
                     TKKILHLNEPVIGYGRLLAEMP"
     gene            1176..1355
                     /locus_tag="VFY96_17860"
     CDS             1176..1355
                     /locus_tag="VFY96_17860"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HEX5608392.1"
                     /translation="MAKILECAKVDPSSGCNQVIRGETTEEVMQKAAEHAKEHGIRQV
                     TPELIEKVKANIRDA"
     gene            complement(1619..3013)
                     /locus_tag="VFY96_17865"
     CDS             complement(1619..3013)
                     /locus_tag="VFY96_17865"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX5608393.1"
                     /translation="MGATEKIAQFIVETSFESIPRNAIEKTKRTTLDCLGAALAGVSE
                     PVSQAVTGYATKLGGPAQASVFGAGVKMSVADAALANGTIAHALDYDDCGVKIGHPSV
                     LVLPAVLSLGEHLGASGKDILAAYIVGLEVEGKLALHADFKLMQARLNHQTWYGSTGA
                     AAASAKLLKLNVEKTRMALGIAANYACGLSVNHGSMAGAMAAGNACRNGLIAALMAEA
                     GITANPNVIEAKNGFYDTLVGPDHYDAERMAESLGKPFYIEIPGIGLKKYPSCYHTHR
                     ALDGVFQLLDEHRLRDQDIAEVDVGTSERAMRVLAFTEPATPYQAKFSMPYCIAAAVV
                     DQQVTLETFTPSKFADRNIVETTKKVHLSFPDVPIWPGLADVGPDTEFVGNPVTIRTA
                     DGRSYSARVDIPRGDPALPLSDDELRSKYRDCARSQLRQEDIARSIDSVLGLEMIGDI
                     RALMATLGSSAPQG"
BASE COUNT          665 a          920 c          873 g          705 t
ORIGIN      
        1 taaaagcttg tcgtcaacat gacatcgcag atgttctgcc cggcgcggct ctcgttaacg
       61 aagcgttcca ccagcgcagc atccgaagcg cgataatagg cagcgtcgat ttttggatag
      121 acctgtttga aagcatcgac cagggtcttg gaatccgctg ctgtgaaggt cgcatacatc
      181 atcagcttgc cttcagcctc cgccttcgcc ttcacatcct gagcattggc cgaaaatcgg
      241 gccccaaaaa aagcaacgcc gagggtcaaa aagactgccg cgatgattcg atgttttcgc
      301 atcactccaa cactccaata ctccaattct atggcatttc ggcgagcagc cgcccatagc
      361 cgatgaccgg ttcgttgaga tgaagaattt tcttggtcgc ccaaacctgt gccggaatgc
      421 tcgacagcac cgtcgtcttc agatcttttt cgagcatgtc gattacaggc aggttgtgcc
      481 agcccgcccc gagcatgtag atgccatccg cgggcccgtg ttcaagaaaa gttttcttgg
      541 cgaagttata gatcgcttcg ggcgcgagct tgccggcatc cttgaacggt acttcgatcc
      601 cattcatcgc cttcacatcg aaaccatcgt cctgcagaaa cttcgcgaag cgtcggttca
      661 tgtcgtcctg gaaataggtg atgccgacga gtcttttcat gcccaatgcg cggaacgcgt
      721 ccacttgagc gatcgtcgcc acggtcactg gaattttgta tttcctgctg agcgagtccg
      781 cgatctcgcg atctgcgccg tggccgcgca ccattgtcgg tggcgcgccc atgatcatca
      841 ccacgtccgc gcctagctcg gcaagccggg caacttttct ttccgccacc gtcagcgctt
      901 cttgcaattc tttttcattt cccgcgcgaa tgcccgcatg cgccggaatg aagccgacgc
      961 cctccggcat cagcttgata aaactttcca tcgatcccgg ccgatacgtc ggcttcacca
     1021 atccaacaat tccccgagca ccgtagtaca tgtgttcgct ccgctcgctt tgcaatttac
     1081 taatcgctcg atatcccagt cgcagcctct tatcaagcac cgattcttgc catagaactt
     1141 agggtcagca gaaaagtagt gaaggaggca atcccgtggc aaagatcctg gaatgcgcca
     1201 aggtcgatcc gtcgagtggc tgcaatcaag tgatacgcgg tgagacgact gaggaagtca
     1261 tgcagaaagc ggctgagcac gccaaggaac acggcattcg tcaagtgacg ccggaattga
     1321 tcgaaaaagt caaagccaac atccgcgacg cctagctaac cgttagctat agatcgacgc
     1381 gcggagccgc cttgaaggct cgcgcctcga tttttgggcg ggtctcgcgc gaaggaagaa
     1441 catcgcaacc tccaagcttc gcgtggcgtg gctgccacag tgagacagta tactttcggg
     1501 aaggtcttcg ttgccggagt attggagatt gttcgccgcg gcgcctacca gacataaacg
     1561 tggattgctc aataacaaag acgtaagaaa catctgcgcg tttatgggcg cgtgaatctc
     1621 aaccctgtgg cgccgatgac cccagagtcg ccatcaacgc ccggatatcc ccaatcatct
     1681 ctagccccag aaccgagtct atactacggg cgatgtcctc ctggcgtagc tgactgcgcg
     1741 cgcagtcgcg gtacttcgat cgcagttcgt cgtcgctcag cggcagagcg gggtcgcccc
     1801 gtggaatgtc tacgcgcgcg ctgtagcttc gtccatcggc cgttcggatc gtcaccggat
     1861 tgccgacgaa ctctgtgtcg gggccgacgt ccgcaaggcc aggccagatg ggcacatcgg
     1921 gaaaagaaag atgaactttc tttgtcgtct cgacgatatt gcgatctgcg aatttggacg
     1981 gcgtgaaagt ttcgagcgtc acctgctgat ccaccactgc ggcggcaata cagtacggca
     2041 tgctgaactt ggcttgatac ggagtcgccg gctcggtgaa ggcaagcacg cgcatggcgc
     2101 gctcggaggt gccgacatcg acttcggcaa tgtcttggtc tctgaggcgg tgctcatcca
     2161 ataactgaaa gacgccatcc agcgcccggt gcgtgtgata gcagctcggg tattttttca
     2221 atccgatgcc gggaatttcg atgtagaagg gcttgccgag actttctgcc atgcgctccg
     2281 cgtcgtaatg gtctgggcct accagcgtgt cgtaaaaacc gttcttcgcc tcgatcacgt
     2341 tggggttcgc ggtgattccc gcttcggcca tgagcgcggc gatcaaaccg ttgcgacagg
     2401 catttcccgc tgccattgcg ccggccatgc tgccgtgatt taccgagagg ccgcaggcgt
     2461 aattggcggc gatgccgagt gccattctcg ttttctcgac attcagcttg agcagtttag
     2521 cggatgcggc agccgcgccg gtggaaccgt accaggtctg atgattcagc cgagcttgca
     2581 tcaacttgaa atcggcatgg agcgcaagct ttccttctac ttcgagtccg acgatgtaag
     2641 cggcgagtat atctttgccc gatgcgccga gatgctcgcc gaggctcagc acggccggca
     2701 gaaccaagac gctcggatgg ccgatcttca cgccgcaatc gtcgtagtcc aaagcatggg
     2761 caatggtgcc gttcgctagc gcagcgtctg ctacggacat tttcacgccc gcgccgaaaa
     2821 cggatgcctg cgccgggcca ccgagcttcg ttgcgtaacc tgtgacggct tgactgaccg
     2881 gctcagaaac accggcaagc gccgcaccga ggcaatcgag cgtcgtgcgt ttggttttct
     2941 cgatggcgtt ccgtggtatg ctctcgaagc ttgtctcgac gatgaactgc gcaatttttt
     3001 ccgtggcacc catgcttcac ctggagacga gatcaggagg gagaaaaacc gcagaggcgc
     3061 ggagtacgct gagatcggag tctgatcatc acaaccgttt taatccgcac tctgcggtct
     3121 cagcgtctcg gcggtgaatc tctgaatgta cgttatcgct tta
//
LOCUS       DASULZ010000868         4291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_913039, whole genome shotgun sequence.
ACCESSION   DASULZ010000868 DASULZ010000000
VERSION     DASULZ010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913039"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..838)
                     /locus_tag="VFY96_17870"
     CDS             complement(170..838)
                     /locus_tag="VFY96_17870"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX5608394.1"
                     /translation="MDGKALVDAVKKEIIEPGIQRLLDLPYFTELRQGKLSTKRMQGW
                     AIQHYLHNKAILKSFALGMVKNAHEQTMFDYFTEQFTEEQTHPDLAKRFGLALGLKEE
                     DFAEATQIFECQIHSARTVYNTLMGYAPQNRASALTSESMVCRYSEEFLNAGRKHYGL
                     SDHDLTFFSVHMVADQEHTARSAELIARMVKTAREERLVRESAQYMVRIKLGKFDGLY
                     REYA"
     gene            complement(907..1851)
                     /locus_tag="VFY96_17875"
     CDS             complement(907..1851)
                     /locus_tag="VFY96_17875"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5608395.1"
                     /translation="MLFYLALSILLQNSATALGQDTRKVRMAYSAFSISFLNIFAARD
                     AGLFKRHGLDVELIQMAGPLPVAALASGEIDYLTGYTTGIVATGQGAPLKGIMISLRK
                     PPFFLIADQTIQRPNDLAGKRIGVDRIGSLQHLVARLMLKQKGVDPEKVIFTQTGSVS
                     NTVSSLAQGVLSAAILSGPHNVIMAQKGFRQIAAADELPMHFPTSGLVVNETRLKTDA
                     PRIKSVIRVMLDATAFCRKERSWVINYVKDRWKVDVKTAETVYDQWLSILTPDGKISV
                     KDMQEYFDLAYSQKQIPAPINVAAVTDYSLLEQVLAGK"
     gene            complement(2007..3224)
                     /locus_tag="VFY96_17880"
     CDS             complement(2007..3224)
                     /locus_tag="VFY96_17880"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX5608396.1"
                     /translation="MQRKANPLLWIVLCLLTMLPTSLPAQQISSNPTPFVVPAGLEPA
                     VEFWKRVFSEFSRSQLIFFDPLDMSKIYEVIEVGEDNRTNAYIDSERERIAAANGVDI
                     ERVRAQRGIKERTAEGIKRSGRYLHQIQQIFRERGLPIELTYLPIVESSYDIGARSSV
                     GALGIWQFMPRTGREYMRVNSLVDERRDPIESSRAAAAYLKQAYDSLGSWPLAVTSYN
                     YGQGGMARAVAQLESDNLVELIEKYEHPYWGFAPKHFYAEFLAAVEIGSNVSQYFPGL
                     VLDSAVAMKEIEVKGKASLGSLISSSGLSRTEFLAWNPALAPTARIVPAGYRVKLPID
                     RATPAIVTVAQVPPSARPAQAPVVHHRVRRGETIYEIARRYGASVENILRINGLRKAH
                     LLKVGSTLRIPKI"
     gene            3493..3996
                     /locus_tag="VFY96_17885"
     CDS             3493..3996
                     /locus_tag="VFY96_17885"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX5608397.1"
                     /translation="METITLVAMSEAALRVILGWRMLVSGISNVRRWPNPVNTASILF
                     PQGAAFFGFVATLLMVVGGSGLAAGIQTPLCAVMIVIFLIPTFALHWHWLKVLPTMSP
                     AVKAAITDDKAQNYFRSFDRQAFHAHEVGIRDNLVFLVAAIYFAVRGSAAFGVDNWLP
                     DWVIWLF"
     gene            complement(4013..>4291)
                     /locus_tag="VFY96_17890"
     CDS             complement(4013..>4291)
                     /locus_tag="VFY96_17890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5608398.1"
                     /translation="RWENVAVIADATAGTAWVAAAPGVTAGQRFLELASMARDRRGRA
                     IIFSAPAELKRGVEVWGESPPTISLMRGVKHQFDPKELLNPGRFLGGI"
BASE COUNT          961 a         1150 c         1162 g         1018 t
ORIGIN      
        1 ataccacacc acctgttttt cctgtttggc atttcccgct aacgaggaga gatccgtttg
       61 ggcgtgagcg ctcgaaaaac ccgcaaccag cgcagccagc atcaaataac gcgtcatgag
      121 tttctccttt ttaccgtctc ggcgtccatg gatcgatttt gccgttccat cacgcgtatt
      181 cgcgataaag cccgtcgaat ttgccgagct tgatgcgcac catatactgt gcgctttcgc
      241 gcaccaagcg ctcttcgcgg gccgttttaa ccatgcgcgc aatcagttcc gccgagcgtg
      301 cggtgtgctc ctgatccgcc accatatgaa cggaaaaaaa cgtcaggtca tgatcgctga
      361 ggccgtagtg cttccgtcca gcgttgagaa actcctccga ataacgacaa accatgctct
      421 cgctggtcag cgcggaggcg cgattttgcg gcgcatagcc catcaaggtg ttataaacgg
      481 tgcgcgccga atggatttga cactcgaaga tctgcgtcgc ctcggcaaaa tcttcttctt
      541 taagacccaa cgccaggcca aagcgtttcg ccagatcggg gtgcgtctgc tcttcagtaa
      601 actgctcggt gaagtaatcg aacatcgtct gctcatgggc atttttcacc atacccagcg
      661 caaaggattt gaggatcgct ttgttatgga gatagtgctg aatggcccag ccctgcatcc
      721 gcttcgtcga aagttttccc tgacgtaatt cggtgaaata aggcaaatcg agcaggcgct
      781 gaatgccggg ttcgatgatt tcttttttga ccgcatccac aagcgctttg ccgtccattg
      841 ctttgctcct tcattcgacg tttttagttt ttctgggttc tagtcaggca agccggaaaa
      901 ctatttttac tttcccgcca aaacttgttc gagtaacgag taatccgtca ccgcggcaac
      961 attgatcggc gctggaatct gtttctggct gtaggcgaga tcgaaatatt cctgcatatc
     1021 tttgacgctg attttaccat ccggcgtgag aatcgacagc cactgatcgt aaacagtctc
     1081 ggcggttttt acgtcgactt tccagcgatc tttaacgtag ttgatgaccc atgatcgttc
     1141 cttgcggcaa aatgcggtgg catcgagcat tacgcggata acactcttga tccgcggcgc
     1201 atctgttttc agccgagttt cgtttaccac gagtccgctg gtgggaaaat gcatcggcag
     1261 ttcatcggcg gcggcgattt gccgaaagcc tttttgcgcc ataatgacgt tgtgaggacc
     1321 cgacaaaatc gcggcggata agacaccctg agcgaggctc gagacggtgt tggagacgga
     1381 ccccgtctga gtgaagatca ctttttccgg atcgacgcct ttttgcttga gcatcaaacg
     1441 ggcgaccaaa tgttgcaggc tgccgatgcg atcgacgccg attctcttgc cggcaagatc
     1501 attgggacgt tgaatcgttt gatcggcgat caggaagaag ggtggcttgc gcaaagaaat
     1561 catgatcccc ttgagaggcg ccccctggcc ggttgcgaca attcccgtgg tgtatccggt
     1621 caaatagtcg atctcaccgc tagcgagcgc agccacagga agcggacccg ccatttggat
     1681 cagttccacg tccaagccgt gccttttaaa cagacccgcg tcacgcgccg caaaaatatt
     1741 aaggaaggag atgctgaagg cggaataagc catgcgcact ttgcgggtgt cttggccgag
     1801 cgcggtcgcg ctattttgca gcaggataga gagcgcgaga tagaaaagca ctctgaaagc
     1861 aaaagaatac ggctgacaaa ttaagcactt gaatctcacg ggccgactat atcgaggtcg
     1921 cggaacttgt caatgtcacg cgcgcgaaca ctcgagtgcg cgcccgatgc actgcaacaa
     1981 actcagttcc ggtggataga attgctttag atctttggga tgcgaagggt gctgccgacc
     2041 ttcaagagat gggctttgcg gagaccgttg atccggagga tgttctcgac agatgcgccg
     2101 taacgccgcg cgatctcgta aatggtctca ccgcgacgaa cccggtgatg aaccaccggt
     2161 gcttgagccg gacgtgcgga gggtggcacc tgagcgacgg taacaatcgc gggcgtggct
     2221 cgatcaatgg gtaacttaac gcgataacct gcggggacga tacgcgctgt gggggcgagc
     2281 gcgggattcc aagctagaaa ttccgtacga cttaaacccg acgagctgat cagtgagccc
     2341 agggaggctt tacctttgac ctcgatttct ttcattgcca ctgcggaatc gagcaccaat
     2401 ccggggaaat attgggacac gttgctgccg atttcgaccg cggccagaaa ctctgcataa
     2461 aaatgcttgg gcgcgaaccc ccagtaggga tgctcgtatt tttcaatcag ctcaacaaga
     2521 ttgtctgatt ctagttgggc gacagcccga gccatcccgc cttggccgta gttataagag
     2581 gtgactgcca gaggccaact tccgagtgag tcataggctt gtttgagata ggctgccgcg
     2641 gcacgcgaag attcgatcgg gtcgcgcctt tcgtcgacca gcgaattcac gcgcatgtat
     2701 tcccggccgg tgcggggcat gaactgccat atgcccaagg ctccgacaga tgatcttgcg
     2761 ccgatatcat aggacgattc cacgatggga agatacgtaa gttcaatggg taaaccgcgc
     2821 tccctgaaaa tttgctggat ctgatgaagg tatcgacccg agcgcttgat cccttcggct
     2881 gttctttcct tgataccgcg ctgagcgcga acacgttcta tatcgacgcc atttgccgca
     2941 gcgatgcgct cccgttcgct gtcaatatat gcattcgttc gattgtcctc gccgacctcg
     3001 atcacttcgt aaattttgct catatcaagg ggatcgaaaa agatcagctg agaacgacta
     3061 aactcgctaa aaactctttt ccagaattcc actgcaggct ccaagccggc gggaacaaca
     3121 aacggtgtgg gattcgatga gatctgttgg gccggcaagc ttgtcggcaa catcgtcagc
     3181 agacacagaa ctatccagag aagagggttc gccttacgct gcatcgctac tccttcgccg
     3241 ggcccgaatc agagcaaccg ccatgccaca gacgacggtg gctaacgcgc cgcataatac
     3301 gcgagttttt cttgttaaac aatgatccct caacgtttga ctggacattt ttgcaaccgt
     3361 tgcgcagtaa cgaaagcaaa atagtatgcg cttcggtgcg gcgcctcgga ctagcctcat
     3421 gaaattgacc gctgtgacgg cgccaattat attgcagcaa atttttgaac cgcgacgcaa
     3481 aggagcattt tgttggaaac aattactcta gtggcgatga gcgaggcagc cctccgggtg
     3541 attctcggtt ggcgcatgtt ggtgagcggt atcagcaatg ttcgccgctg gccgaatccg
     3601 gtaaatactg cgagtatttt gtttccgcaa ggcgctgcgt tcttcggctt tgtcgccact
     3661 cttctgatgg tggtcggagg gtccggtctt gccgcaggaa ttcaaacacc cctatgcgcc
     3721 gtgatgattg tgatatttct tataccgacc tttgccttac attggcattg gctcaaggta
     3781 ttgccgacca tgtcaccggc ggtaaaggcg gcaattacag atgacaaagc gcagaactat
     3841 tttcgttcgt ttgatcgtca agcgttccac gcccatgaag tcgggatccg tgacaacctg
     3901 gtttttctcg tggcggcaat ttattttgct gtccgcggaa gcgcggcatt cggcgtcgat
     3961 aattggctac cggattgggt gatatggttg ttctgaaaaa ctcaaggagc ctctagatcc
     4021 cgcctaagaa tcgcccaggg ttgagcaatt ccttcgggtc gaactggtgc ttcacgccgc
     4081 gcatgagcga aatcgtcggc ggcgattctc cccagacttc cacgccgcgc ttgagttcag
     4141 cgggcgcgga aaagatgatc gcgcgcccgc gacgatcacg cgccattgat gcgagctcca
     4201 aaaatcgctg ccctgctgta acacctggcg cggccgcgac ccacgctgtg ccggcggtcg
     4261 cgtcggcaat gactgctacg ttttcccacc g
//
LOCUS       DASULZ010000869         3955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_403500, whole genome shotgun sequence.
ACCESSION   DASULZ010000869 DASULZ010000000
VERSION     DASULZ010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403500"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VFY96_17895"
     CDS             complement(<1..441)
                     /locus_tag="VFY96_17895"
                     /inference="COORDINATES: protein motif:HMM:NF017517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEX5608399.1"
                     /translation="MRPIDIFRRVELPAPISGRLLLHSMPGRFESLDNVWQHLERESV
                     GAIVCLNEPHEIRLKSSRYADALESGSVPCPVLSFEIREGGVPEDRSAFWALANDLAK
                     RLQAGEAVLIHCAGGMGRTAMLAVAVLLALGEPRDAAEQRVSRAG"
     gene            complement(616..1632)
                     /locus_tag="VFY96_17900"
     CDS             complement(616..1632)
                     /locus_tag="VFY96_17900"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5608400.1"
                     /translation="MRFDRKSLTVPALGFAILLYGALTSFSQERPTILAGHGTLSGSI
                     LPLWVGADAKLFEKYGLQVKPIYLPRAAGRPALLSGDIHVYFSAGPPLVQMRLGGADV
                     AVTSCVAHKLTSKIMVSPTIQKIADLRGKVLAVANPGSASDFAAKLFLAREGMKAGQD
                     VALIYSGSTSAAFAALVNGRVQAIFATSPNDLQATQAGFKALLELGDLNIPYAGNCTA
                     AMRPYIAKQQARMRSFVAGITEAIAYTRSHPSEAKAILQKYTRVSDPTVLQHAYESDT
                     RYMEPVPRPTAEGTKTILENLGVSGRAAEQFVAEFNDNSFIRQIVDEGFLKRLYPGGI
                     PALR"
     gene            2044..2577
                     /gene="def"
                     /locus_tag="VFY96_17905"
     CDS             2044..2577
                     /gene="def"
                     /locus_tag="VFY96_17905"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX5608401.1"
                     /translation="MSILKVSRLGHPVLRKVTANVPPEVLQSASMQKLIDDMIDTMKE
                     YDGVGLAADQVHESLQLAVLEVAENPRYPDKPRVPLTVLVNPRITPLVEETEDDWEGC
                     LSIPDLRGVVPRYKSVRVQAYDRDGQPLDFVAHDFHARVIQHEFDHLNGKVYLDRMRD
                     LTTLTFLQEFTRYWLRS"
     gene            2701..>3955
                     /gene="cofH"
                     /locus_tag="VFY96_17910"
     CDS             2701..>3955
                     /gene="cofH"
                     /locus_tag="VFY96_17910"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HEX5608402.1"
                     /translation="MNSSQLESSLERLLRTVSPQTAEILDHALSGKDITIDEATRLFD
                     ADGSDLLALIAAADDLRSRTVGDVVTYVINRNINFTNVCVKACGFCAFSRGHLAEEGY
                     YLPIEEIIRRAQEARELGATEVCVQAGLAPGMDGWHYVNLCRALKEALPDLHIHGFSP
                     EEVLYGATLSGVTVRDYLIALREAGVGSLPGTSAEILVDAIRQRVSPGRIATAQWIEL
                     IQTAHEVGIPTTSTMMYGFVESSRDRSVHLDILRQIQRRTGGITEFVPLSFVYEEAPM
                     FHRKPPAGLRRGASGAEVMKMYAVSRLMLNNTIPNLQVSWVKEGPKLSQIALLAGAND
                     FGGTLINESISTAAGASYGQLMKPSQFRGMIREMSRIPAERSTTYEKIRVFESEAQHR
                     DLLDEVNDPQQRFGSYQNLIQLKQYR"
BASE COUNT          862 a         1163 c         1078 g          852 t
ORIGIN      
        1 ccccgcacgt gaaactcttt gttccgcggc atctctgggc tcgcccaaag caagcagcac
       61 cgcaaccgca agcatagcag tacggcccat accgcccgca caatggatca acaccgcttc
      121 ccccgcttgc aaccgtttgg ctaggtcgtt ggccaaagcc caaaacgcgc tccggtcttc
      181 cggtaccccg ccttcacgaa tctcgaatga cagcactgga caaggaacgc ttccggattc
      241 gagggcgtcc gcatagcgcg atgatttcag gcggatctca tggggttcgt tcaagcaaac
      301 gattgctccc acggattcgc gctcgagatg ttgccagacg ttatcgagac tttcgaagcg
      361 gcctggcatg ctatgcagca gcaagcgccc ggagattggc gcgggaagtt cgacacgacg
      421 aaatatgtct atcggcctca taaacgctcg ttgttcgtca aaaagtactt ttctgctcgt
      481 gatccgttgc gcagcgattc cagactgtct ctggacattt gatgagtcat cggctaccag
      541 ctcccatgcc gccgcaatgg aacagtgatg cttcggagcc acttcacggc tggggcaaga
      601 aaaacctcgt acgcgtcacc gcaacgccgg gataccaccg gggtaaagcc gcttcaaaaa
      661 gccctcatcg acgatctgcc tgatgaacga attgtcgttg aattcggcga caaactgctc
      721 ggccgcacgg cccgaaacgc cgagattttc caggatcgtc ttggtgcctt ccgccgtcgg
      781 acgcggcacc ggttccatgt agcgggtgtc ggattcgtag gcgtgctgca gaacagtcgg
      841 atcggaaact cgcgtatact tctgcaagat cgccttggcc tcgctgggat ggcttcgagt
      901 atacgcgatc gcttcggtga tgccggcaac aaagctgcgc atccgcgcct gctgcttggc
      961 gatgtaaggg cgcatcgccg ccgtgcagtt gccggcataa ggaatattca agtcgcccaa
     1021 ttccagcagc gccttgaatc ccgcttgcgt cgcctgcaaa tcgtttggcg aggtcgcgaa
     1081 aatagcctgc actctgccgt taaccagtgc cgcaaacgcc gcgctcgtag agccgctata
     1141 gatgagcgca acatcctgac cggctttcat gccctcgcgc gcgagaaaca gtttcgcggc
     1201 aaaatcactg gccgacccgg gattcgccac ggccaaaact ttgccgcgta aatcggcgat
     1261 cttttggatc gttggactca ccatgatctt cgaggtgagt ttgtgtgcga cgcaggaagt
     1321 gaccgccaca tcggcgccgc ctagacgcat ctgcaccaga gggggaccgg cagaaaaata
     1381 aacgtggata tcgccgctca gaagcgccgg tcgacccgcc gcgcgcggca agtagatggg
     1441 cttgacttgc aagccgtatt tctcgaacag cttcgcgtcc gcgccgaccc aaagcggcag
     1501 gatcgatccc gataatgtcc catggcctgc aagaatggtc ggccgttcct gactgaaaga
     1561 agttaacgcg ccgtagagca gaatagcgaa gccgagcgcc ggcacggtca gggatttacg
     1621 atcgaatctc atcctcgaag cagcaaaacc aaagccgcga ttctgtttgt acagtctctg
     1681 tattctgaaa tccagattgg gcgtgttcac ataaatcctc cggctcggcg ggatgattac
     1741 gcgacggctt cattcaatcc aaaggtcgcc gttatcgcgg atcgcttgca ggacctctcg
     1801 cgtgcttgac agcgttgcca cagctcacga aggttactac gagcgcgttc gggccatggt
     1861 aatcagaaaa taatggtttc gatccatgtc atgtgcgtcg gttcggtcca tctgaaatga
     1921 catcatcgct ggcgaacttc aatagcagca cactttaatc tcacttattc cgaggcccca
     1981 cggcggcgtt cgcgatcgac agcggctctt tttccacatc ttcgcctgtg ctagagcaat
     2041 tccatgtcga ttctcaaagt cagccggctc ggccatcccg tattgcgtaa agtcaccgca
     2101 aatgtgccgc cggaagtcct gcagtccgcg agcatgcaaa aattgatcga cgatatgatc
     2161 gacacgatga aagaatacga cggtgtcggc ttggccgctg atcaagtgca tgaatccctg
     2221 caactcgcag ttttggaggt cgccgaaaac ccgcgttatc cggacaagcc gagagtgccg
     2281 cttacggttt tagtcaatcc gcgcatcact ccgctcgtcg aagaaacgga agacgactgg
     2341 gaaggttgtc tcagcattcc ggatctgcgc ggggtggtgc cgcgttataa gagcgtccgg
     2401 gttcaagcct acgaccgcga tggccaaccc ctcgactttg tcgcacacga ttttcacgcc
     2461 cgagtcatcc agcatgagtt cgatcaccta aacggcaaag tctacctcga tcgcatgcgc
     2521 gatctgacga cgctcacttt tctccaagaa tttacccgtt actggctgcg ctcctagcaa
     2581 gccgctggaa atgatcctta ggctgcattg cgctccaagc cttgcgcgcg ttgattttgt
     2641 cgcgcgtgca gtcctgccag tatcgttgag tttgcttgac cgaaacgtta gagtcgccgc
     2701 atgaattctt ctcagctcga atcttcttta gaacgactgc tgcggacggt ctcgccccaa
     2761 accgcggaaa ttttggatca cgcactttcc ggtaaagata tcacgataga cgaagctacg
     2821 cggctcttcg atgccgacgg ttccgatctc ttggcactga ttgccgccgc cgacgatcta
     2881 cgctcgcgca ccgtcggcga cgtcgtgact tacgtcatca atcggaacat caacttcacc
     2941 aacgtttgcg tcaaagcttg tggcttttgc gcgttcagcc ggggtcattt ggctgaggag
     3001 ggatactatt tacccatcga agagattatt cggcgcgcac aagaagcgcg cgaattgggc
     3061 gccaccgagg tttgcgtgca agcaggtctg gcgcccggga tggatggctg gcattacgtc
     3121 aatctgtgcc gcgcgttgaa agaagcgctg ccggatctgc acatacatgg gttttcgccg
     3181 gaagaagtgc tttacggcgc caccttgagc ggcgtcactg tccgcgacta cctgatcgcg
     3241 ctcagggaag ccggcgttgg cagcttgccc ggaacctccg cggaaattct cgtcgatgcg
     3301 attcgccagc gggtctctcc gggccgcatc gccacggcac agtggatcga gctcatccaa
     3361 accgctcatg aagtcggcat ccctaccacc tcgacgatga tgtatggttt tgtcgaaagc
     3421 tcacgcgaca ggtcggttca cttggacatc ttgcggcaaa ttcaaagacg caccggcggc
     3481 atcaccgagt tcgtgccgct gagctttgtt tacgaagaag cgcccatgtt tcatcgcaag
     3541 cccccggctg ggctgcggcg tggcgcaagc ggcgcggagg tcatgaaaat gtatgcggtg
     3601 tcgcggctga tgctgaacaa cactattccc aatctgcagg tctcgtgggt caaggaagga
     3661 ccgaagcttt cgcagatcgc gctccttgcc ggagccaatg acttcggcgg cacactgatc
     3721 aacgagagca tctcgactgc agccggtgct tcttacggac aactgatgaa accgtcccag
     3781 tttcgcggca tgattcgtga aatgagccgt attccggccg agcgctccac cacctacgaa
     3841 aaaatccgtg tattcgagtc tgaagcgcag caccgcgatc tgctcgacga ggtgaatgat
     3901 cctcagcagc gcttcggatc ctatcaaaat cttatccaac tgaagcaata tcgtt
//
LOCUS       DASULZ010000870         8105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_506468, whole genome shotgun sequence.
ACCESSION   DASULZ010000870 DASULZ010000000
VERSION     DASULZ010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 8105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8105
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506468"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VFY96_17915"
     CDS             complement(<1..401)
                     /locus_tag="VFY96_17915"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5608403.1"
                     /translation="MIEGLTDKVVIVTGGAHGIGKAYCLAFAKAGAQVVIADIDKAGA
                     AIAASEISKANSGQGQPKSLVSPLALYVDVANEAATKEMAARTVDRFGRVDVLINNAA
                     VFSVVPMNRGRIETIDPEEWDRLMAVNLRGP"
     gene            complement(494..1588)
                     /locus_tag="VFY96_17920"
     CDS             complement(494..1588)
                     /locus_tag="VFY96_17920"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX5608404.1"
                     /translation="MATARGATLLGPDKLEIREYPIPDIPPDGGLLKVEMGGVCGTDV
                     KYLHGKLQVPYPIILGHEILARVEKLGRNAAQIHKVKEGDRVILKGALGCGRCADCRR
                     NNQRFCRQRTNYGGRTKCDQPPHLFGGFADYVYLAPDVLATKVSDALPAEAAALIGSV
                     MANGFQWAIRRGGVKMGDYVLIQGPGQQGLSCTFASKHAGAAKIFVTGIGRDRPRLEL
                     AEKFGATRTINVEEEDVVEVVRRETNGEMCDVVVDVSGNPNAILKSVDCLHRQSTMVL
                     GGLTGDTTVTPMLMDKLVWNEIKLQGCFTADNDATEAALRIIEETKFPVEQMVSHVFP
                     LAQIENCIRAIGGEVPGTFPTKALINPAIG"
     gene            complement(1717..2697)
                     /locus_tag="VFY96_17925"
     CDS             complement(1717..2697)
                     /locus_tag="VFY96_17925"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5608405.1"
                     /translation="MKNFLSVLAFTAILIFSTVAPSRSAEINVVWTSEVGQFAPLWIT
                     KEARLFEKYGNSVQLIFIQGASSAAAALSSGDAQVGMFSPQVVISTPALDLVMFGRLG
                     NSMDNRIFARKGIKSLKEVKRVAISRFGSNADFAARYLLQREGLKPDIDVALLQVGNQ
                     SNRIVAVETNNADAAMLTPPMTLQARKLGLTLLVDASKLNIPYSSLMFVARRPYLTKN
                     RADLVNFTKAMIEGVAYYKANKEFSLKVLSKYMKVQDREVLEENFREYDFPLKPYPAR
                     EYFELPIQEVGKKDPKVLKENPERYADMSLVKEIESSGFIDKIMQEYRVK"
     gene            2795..3772
                     /locus_tag="VFY96_17930"
     CDS             2795..3772
                     /locus_tag="VFY96_17930"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5608406.1"
                     /translation="MRPIIALIIVLLLHEAAFGQKATRVMSGYAATSGPHAVLWIARD
                     AGLFEKNGLRTEVAYIRSGSTMSQALVAGEIQMAQMGGPAMLAAGVAGMDVTFVAVAL
                     NTTPIVIMGTVSRIEELKGKAVGITRFGSNTDISARFAIRKAGLQPEKDVALVQLEDY
                     PGIMGGIASGRIAAGALADPFTDAAKKMGYKEIADIAAMGLEFPFVGIATKKSYIKDN
                     TDTVQRFVRAYTEAIALYKNNRDLAMKVTSKYTGIKDSATLASTVDFYAPKLQRVPQA
                     TVGGLKFVLEQVGVRDARAKNYSPESFMDNRFVKQLEDNGFIKALYPKG"
     gene            complement(3859..4317)
                     /locus_tag="VFY96_17935"
     CDS             complement(3859..4317)
                     /locus_tag="VFY96_17935"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX5608407.1"
                     /translation="MAVALWVGAMVFFLVVLGPSANALEPRIAIQTLNRGRIGFELLS
                     WIAIALLLLTGVFNLLQRLSISTLATREFYSVILTIKLFVFGAMLAHHLLQTFRYAPA
                     IARLTSELPELPNHWPEGLLGQWRRWFLLLKINAALGPIAILLGLNLVKV"
     gene            4511..5176
                     /gene="coxB"
                     /locus_tag="VFY96_17940"
     CDS             4511..5176
                     /gene="coxB"
                     /locus_tag="VFY96_17940"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX5608408.1"
                     /translation="MLQWLPEDVSTFGQEIDSLFYLIYYITAVTFVLVTVLMVVFLIK
                     YRAQSGRRATYTHGNTTLEIIWTIIPAAILIVLAFMSASTWAKVKRHVPETDFEVGVT
                     AKQFNWEVSYPGPDGKLGTDDDVKFDNDLHVPVHKPIRLVIASRDVIHSFFIPNMRFK
                     QDAVPGRQIINWFEGTKAGKYELPCAELCGFGHSGMKGWLYVHTPEEYKKWADENLAA
                     KSQ"
     gene            5230..6990
                     /locus_tag="VFY96_17945"
     CDS             5230..6990
                     /locus_tag="VFY96_17945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX5608409.1"
                     /translation="MTETAAAHASHEHHQELGFIRTYIFSLDHKMIGRQFLFMGLLMI
                     IVGGLLAMLVRWELAWPETPVPGLGIIPEPYIYPGEHGGGIIPPQTYNALFTMHATIM
                     IFFVVMPIMVGTFGNFLIPLMIGTRDMAFPKLNMLSFWVGAVATLVMLSSFFVPGGPA
                     AGGWTSYATLSARAQYTGVEWGQHLWIISLLILGVSSLMGSINYITTIINMRAPGMTY
                     FRMPLTVWSLFITAILLLLALPVLTAALAMLLFDRMAGTSFFLPEGGGQPLLWQHMFW
                     FFGHPEVYILVLPAMGVTSDILSTFSRKPIFGYHAMAFSMIAIAFLSWIVWGHHMFVS
                     GMNPLLGTAFMMTTMVIAVPSAIKTFNWLGTLWGGNIRFASPMLFALGFVSNFIIGGL
                     SGVFMASTPVDIFIHDTYYIVAHFHYVVAGIVFALFAAVYYWFPKMFGRKMNEGLAKI
                     HFVLTYIFFNGAFFPMHFLGVAGHMRRIYNPTQYEFLLPMQHWNVFITVSALLLGASQ
                     FIFLYNFIFSLFAGKKAERNPWQANTLEWSAPTPPPHGNFEVQPVVYRGPYEYSSPEV
                     EEDWLPQDRNLGTAAAARAH"
     gene            6999..7970
                     /locus_tag="VFY96_17950"
     CDS             6999..7970
                     /locus_tag="VFY96_17950"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX5608410.1"
                     /translation="MKSDPRRMGTPASPRPSLISIWPHRLAVVLVCATFPLLFIGGLV
                     TSKGAGLAVPDWPTTFGHNMFFYPWSKMVGNIFYEHSHRLVAACVGLLTIALTVTVWL
                     RESRAWLRWLAVAALVLVIVQGILGGLRVVLLEQTLAIVHAATAQAFFALTIALAVFT
                     SKEWLSLPEPTKSVNDGGRLRRLCLATTALIYMQVIIGAVLRHTGERVDVHLALAALV
                     ALHTMLILIRISRNHASVYELQRPALALFALLLLQLLLGTLSYLAKFTTGLRLPIDAT
                     VILTTTHLACGALMLGTAVALTLKCYRLSTARTQSAGREILTEQFST"
BASE COUNT         1723 a         2191 c         2251 g         1940 t
ORIGIN      
        1 aaggggccgc gcaggttgac cgccatgagc cgatcccatt cttccgggtc gatcgtttcg
       61 attcggccac ggttcatcgg cacgacggaa aaaaccgcgg cgttgttgat cagtacgtcg
      121 acccggccga agcgatcgac agtgcgggcg gccatttctt ttgttgcggc ttcgttcgcg
      181 acgtcgacgt aaagcgcgag tggagagaca aggctcttcg gctgtccctg gccgctgttt
      241 gccttgctta tttcactggc cgcaatagct gcgccggctt tgtcgatatc ggcgatcaca
      301 acctgggcgc cggctttcgc aaaagctaaa cagtaagctt taccgatgcc gtgggcaccg
      361 ccggtgacga tcacgacttt gtccgtgagt ccttcgatca ttcaactcta atcttgaagc
      421 aatggagtgt tgggtaatgg agtaatcgga gggaggccaa acaacactcc agtactccat
      481 cactccagtt gtgttatccg atcgccggat tgattaatgc cttagtcggg aaggttcccg
      541 gcacttcgcc gccgatggcg cgaatgcaat tctcgatctg agcaagcgga aatacgtgac
      601 tcaccatttg ctcgacgggg aatttggttt cttcaatgat ccgcagtgcc gcttcggtgg
      661 cgtcattgtc cgcagtgaaa cagccttgta atttgatttc gttccaaacg agtttatcca
      721 tcaacatcgg cgtcacggtg gtatcgccgg tcaatccgcc cagcaccatc gtcgattgac
      781 ggtgcaggca gtcgacggat ttcaaaatcg cgttgggatt gccggagaca tcgactacca
      841 catcgcacat ctcgccgttg gtctctctgc gcaccacctc gaccacgtct tcttcttcaa
      901 cattgatggt ccgcgtcgcg ccaaatttct cggcgagctc gagccggggg cgatcgcgac
      961 cgatgccggt gacgaatatt ttggccgcgc ccgcgtgctt cgaagcaaat gtacacgaga
     1021 ggccctgctg tccgggaccc tgaatcaaaa cgtaatcgcc catttttacg ccaccgcggc
     1081 ggatcgccca ttgaaagccg ttggccatca ctgaaccgat gagagctgcc gcttcggcgg
     1141 gaagcgcgtc gctcaccttg gtcgcgagca cgtccggagc aagataaaca tagtctgcga
     1201 atccgccgaa gagatgcggc ggctggtcgc atttggtgcg cccgccgtag ttggttcgct
     1261 gccgacagaa gcgctggtta ttgcgccggc agtcggcgca gcgcccgcaa cccagcgcgc
     1321 ctttgagaat gacccggtcg ccttctttaa ctttgtgaat ctgagcagca ttgcgcccaa
     1381 gcttttcaac ccgcgccaaa atttcgtgac cgagaatgat cggataggga acctgtaatt
     1441 tgccatgcag gtatttgacg tccgtgccgc acacgccgcc catttcgacc tttagtaatc
     1501 cgccatcggg tggaatgtcg gggatcgggt attcacgaat ctctagtttg tccggaccga
     1561 gcaaagtcgc tccgcgcgcg gtggccatgg ttgttctcct ggctaatcgg tttgagttag
     1621 ctgatccctc gactcgttcc taaagatact tggcgtactc aggcatcaga tccttcgctt
     1681 tgctcaggat gaaatgatct ccgctcttga cggagatcat ttcactctat attcctgcat
     1741 gatcttatcg ataaagccgc tgctctcgat ctctttcacc aggctcatgt cggcgtagcg
     1801 ctcgggattt tccttgagca ctttggggtc ttttttaccg acttcctgga tcggtaattc
     1861 aaaatattcg cgcgccggat aaggcttcag cgggaaatcg tactcgcgaa aattttcctc
     1921 caacacttcc cggtcctgaa ctttcatgta cttggacaaa actttgaggc tgaattcctt
     1981 attcgctttg taataggcca caccttcgat catcgctttg gtaaagttga ctagatcggc
     2041 gcgatttttc gtcaagtacg gcctgcgtgc gacgaacatt aggctggaat agggaatgtt
     2101 cagctttgaa gcgtcgacga gcagtgtcaa gccgagcttg cgcgcctgta atgtcattgg
     2161 cggtgtaagc atcgcagcgt ccgcgttgtt ggtttccaca gcgacaatgc ggttcgattg
     2221 attgccgact tgaagtagcg cgacgtcgat gtcgggtttc aatccctcgc gttggagcag
     2281 atagcgggcg gcaaaatcgg cattgctgcc gaagcgactg atcgccacac gcttgacttc
     2341 cttgaggctc ttgatgcctt ttctcgcgaa aatgcggtta tccatcgagt tgccgaggcg
     2401 gccgaacatg accaaatcaa gggcaggcgt cgagatcacg acctgcggcg aaaacatacc
     2461 gacctgcgca tcgccggaag aaagcgcagc ggcagcggaa gaagcgcctt ggataaaaat
     2521 caactgaaca ctattgccgt atttttcgaa gagccttgct tccttggtaa tccagagtgg
     2581 cgcgaactgt ccgacttcac tggtccagac gacattaatt tcggcagatc gagatggagc
     2641 aaccgtactg aaaatcagaa tcgccgtaaa cgcgagaacc gacaggaaat ttttcattcg
     2701 tgacctttcg aatcgctgcg gactgcggtt gatctatgtt ccagcatacc cctatactgc
     2761 cgaacaatcg ttgaatcaag ttgaggagga ctgcatgcgg ccaatcattg cgttgatcat
     2821 tgttctgttg ctgcacgaag cggcgttcgg ccaaaaagcg actcgcgtga tgtccggcta
     2881 cgctgcgaca tcaggaccgc atgctgtgct ctggattgcc cgcgatgcgg gcctgttcga
     2941 aaaaaacggc ttgcgcaccg aagttgctta cattcggagc ggctcgacca tgagtcaagc
     3001 gttagtcgca ggagaaattc agatggcgca gatgggtggg cccgcgatgt tggcggcggg
     3061 ggtcgccgga atggatgtca cgttcgtcgc ggtggcattg aacaccacgc cgattgtgat
     3121 catgggcacg gtctcaagga tcgaagagtt aaaaggcaag gccgtgggca ttacccgctt
     3181 cggctccaat acggatattt ctgcgcgctt cgccattcgc aaggctggat tacaacctga
     3241 aaaggacgtg gcgctggtcc aattggaaga ctatcccggt atcatgggag gcatcgcgtc
     3301 cgggcgcatc gcggcggggg cgctcgcgga tccgttcacc gacgccgcca aaaaaatggg
     3361 ctacaaagaa attgctgaca ttgcggcgat gggcctagag tttccattcg tcggtatcgc
     3421 gacaaaaaaa tcgtacatta aggacaatac cgataccgta caacgtttcg tacgggcgta
     3481 caccgaggcc atcgcgctgt acaaaaataa ccgggacttg gcaatgaagg tgaccagcaa
     3541 atacacaggg attaaagact cggcaacgtt agcctcgacg gtggattttt acgcgcccaa
     3601 gttgcagcgc gtgccgcagg cgaccgtggg cggactcaaa tttgtgctcg agcaggtcgg
     3661 tgtgcgcgat gcacgggcga aaaactactc accggaaagt ttcatggaca atcgttttgt
     3721 gaaacagctc gaagataacg gcttcatcaa agcgctttat ccgaagggtt aacggagtca
     3781 cagccagcaa gtttcatgat ggctgccgac cttgtcgggc cttagtgtcg gcgatgtcgc
     3841 tctgcccgtc atcaattttc aaacttttac cagatttagc cctagcaaga ttgcgatcgg
     3901 gccgagcgcg gcgttgatct ttagcagaag aaaccaacgg cgccattggc caagcaggcc
     3961 ttcgggccag tgatttggca attccggcaa ttcggaagtc agccgggcga tcgccggtgc
     4021 gtatctgaac gtctggagta agtgatgggc aagcatcgcg ccgaagacaa aaagcttgat
     4081 ggttaaaatc acggagtaga attcccgggt agcaagcgtg gatatcgaca agcgctggag
     4141 caggttgaac acaccggtta agagcaaaag cgcgattgca atccaggaca gcagttcgaa
     4201 accgatgcgg ccgcgattga gcgtctggat ggcgattctc ggctccaagg cgttcgcaga
     4261 cggcccgagc acgacgagaa aaaaaaccat tgcgcccacc cagagggcaa cagccatgat
     4321 gtgaatcagc gtggagatta attgcaatgg gagcatggga tcgatttcgc tgcgttctca
     4381 gcgcgcgtag actgcggcgc aaatccgcgc cgaaacgagc gcaagcgcgc ttgaaaagga
     4441 caggtgcgtg tgttagctaa ggaaaagttt tcactcccgt ttcttaacaa aattaaggat
     4501 ggttcactag atgctccagt ggttgccgga agatgtgtcg acgttcgggc aagaaatcga
     4561 ctccttattt tatctcatct attatattac cgccgttact ttcgtgctcg tgaccgttct
     4621 gatggtggtg tttttaatca agtatcgcgc gcaaagcgga agacgggcca cctacactca
     4681 cggcaacacc actctggaga tcatctggac cattattccc gccgcaattc tcattgtgct
     4741 ggcattcatg agcgcgtcga catgggccaa agtcaaacgc catgtcccag agacggattt
     4801 cgaagtgggc gtgaccgcga aacagttcaa ctgggaagtc agttacccgg gtccggacgg
     4861 aaagcttgga accgatgacg acgttaaatt cgataatgat ctgcacgtcc cggtccataa
     4921 acccatacgt ttagtcatcg cctcgcgcga tgtgattcac agctttttca ttcccaacat
     4981 gcgcttcaag caagacgcgg tgccgggccg gcaaatcatt aactggttcg aaggaacgaa
     5041 ggcgggaaaa tatgagctgc cctgtgcaga gctctgtggc ttcggccatt ccggcatgaa
     5101 gggttggctc tatgtgcaca cgcccgaaga atacaaaaaa tgggcggacg agaatctcgc
     5161 ggctaaatcg cagtaagcac ttctagcact tgccatcgat ccgaacgcat taacgaagga
     5221 gttgtagcga tgaccgaaac agcggcggcg cacgcgtcgc atgagcacca tcaagagttg
     5281 ggttttattc ggacctatat tttttccctc gatcacaaaa tgatcgggcg acaattcttg
     5341 ttcatgggtt tgctcatgat catagtcggt ggattgctcg ctatgttagt tcgctgggag
     5401 ctagcttggc cggaaacacc ggtgccggga ctcggcatta tccccgaacc gtatatctat
     5461 ccgggcgagc atggcggcgg aatcattccg ccgcagacct acaatgcgct tttcaccatg
     5521 cacgccacca tcatgatctt ttttgtggtg atgccgatca tggtcggcac cttcggtaat
     5581 ttcctgattc cactgatgat cggcacgcgc gatatggcgt ttccgaaatt gaacatgctc
     5641 tcgttctggg tcggcgccgt cgcaacccta gtcatgcttt cgagcttctt cgttcccggc
     5701 ggaccggcgg cgggcggttg gaccagttac gcaacattat cggcacgagc ccaatatacc
     5761 ggcgtggaat gggggcaaca cctctggatc atcagcttgt tgatcctcgg tgtctcctca
     5821 ttgatgggat ccatcaacta catcacgacc atcatcaata tgcgcgcacc gggcatgacc
     5881 tactttcgta tgccgctgac ggtctggtcg ttgtttatca cggcgatttt gctgctgctc
     5941 gcgctgccgg tccttacggc cgccttggcc atgttgctgt ttgaccgcat ggcgggtacg
     6001 agcttctttc tgcccgaagg cggcggacag ccgttgctat ggcagcacat gttttggttt
     6061 ttcggccatc cggaagttta cattctggtt ctgcccgcta tgggagttac gtctgacatc
     6121 ctctcgactt tttcacgaaa gccgatcttc ggctatcacg ccatggcgtt ctcgatgatc
     6181 gcgatcgctt ttctctcgtg gattgtctgg ggccaccata tgttcgtgag cggtatgaat
     6241 ccactgctcg gcacggcatt catgatgacg acaatggtca ttgcggtgcc gtcggcaatc
     6301 aaaactttca actggctggg gacgctctgg ggcggcaata ttcgttttgc atcgccgatg
     6361 ttgttcgcgc tgggcttcgt ctcgaacttt atcatcggcg gtttgagcgg tgttttcatg
     6421 gcatcgacgc cggtcgatat tttcattcac gatacctatt acatcgtggc gcattttcac
     6481 tatgtggtcg ccgggatcgt tttcgcgttg tttgccgcgg tttactactg gtttccaaag
     6541 atgtttggcc gcaagatgaa cgaaggattg gccaagatcc atttcgtgct gacctacatt
     6601 tttttcaacg gggcgttctt cccgatgcat ttcctcggcg tggcgggcca tatgcggcgc
     6661 atctacaatc ccacgcagta cgagtttctc ttgccgatgc agcactggaa cgtgttcatc
     6721 accgtgagcg cgctcctgct cggcgcctcg caattcattt ttctgtataa ttttatcttc
     6781 agcctgttcg cgggtaagaa agccgaacga aacccctggc aggcaaacac gctcgaatgg
     6841 tccgcgccaa caccgccgcc gcacggcaac ttcgaagtcc agccggtggt ctatcgcggg
     6901 ccttacgaat acagctcgcc ggaagtcgaa gaggattggc tgccgcaaga tagaaatctc
     6961 ggtactgcgg cggcggcccg ggcgcactaa gcggtaacat gaaatccgat ccgcgaagga
     7021 tggggacacc tgcgagtccc cgtccttcac tcatttcaat ttggccgcac cgccttgcgg
     7081 tcgtgcttgt ctgcgcgaca tttccactgc tcttcatcgg cggcttggtt accagcaaag
     7141 gtgcgggact cgccgtaccg gattggccga cgaccttcgg ccacaatatg tttttctatc
     7201 cctggtcgaa aatggtgggc aatatttttt acgaacacag ccatcggctc gtggctgcat
     7261 gcgtcggctt gctgacgatc gcattaacgg tcacggtctg gctgcgcgag tcgcgcgcgt
     7321 ggttgcgctg gctcgctgtt gcggcactgg tgctggtgat tgtgcagggg attttgggcg
     7381 gtttgcgagt cgttttgctc gagcaaacgt tggcgattgt gcacgccgcg accgcacagg
     7441 cttttttcgc gctgaccatc gccctggcgg tatttacttc gaaagagtgg ctcagtttgc
     7501 ctgaacctac gaagtcggtg aacgatggcg gccggctccg ccggttgtgc ctggcgacaa
     7561 ctgcgctaat ctatatgcag gtgattatcg gagcggtgct ccgccataca ggcgagcgcg
     7621 tggatgttca tctggcgttg gcggcgttgg tggcattaca tacgatgcta attctcatcc
     7681 gcatttctag aaatcacgcc agtgtctacg agttgcagcg accggccctg gcactttttg
     7741 cgcttttact gctgcagttg ctgttgggaa ccctgtcgta cctcgccaaa ttcaccactg
     7801 gattgcgcct gcccatcgac gccaccgtga tcctcacgac cacgcatctt gcctgcggcg
     7861 cgctcatgct cggcaccgcc gtcgcgctta cgttgaagtg ctatcgactg tcgacggcgc
     7921 gaactcagag tgctggtaga gaaattctca ccgagcaatt ttccacatga gcatcacgac
     7981 cgtaacggta cagatcgcgc acgccgcgac tgcccgaagg gcggccgact tccttgaact
     8041 caccaagccg cgcattgttt tcatggtgct gattacagcg ctagtgggct actatgcagg
     8101 gtccg
//
LOCUS       DASULZ010000871         5430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_506620, whole genome shotgun sequence.
ACCESSION   DASULZ010000871 DASULZ010000000
VERSION     DASULZ010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506620"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VFY96_17955"
     CDS             <1..272
                     /locus_tag="VFY96_17955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608411.1"
                     /translation="ELIVDADEFVVRCGDPNPPYTGILTLHYSPQRITILDREGQARR
                     RQMKPYFRFDSLPEILRAAGRYVDSRRGGDPYGHKLFCIGSGHTA"
     gene            complement(452..607)
                     /locus_tag="VFY96_17960"
     CDS             complement(452..607)
                     /locus_tag="VFY96_17960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608412.1"
                     /translation="MLDEIALTIGLPFCTVGIGLAVIGMVNLFKSRRRRRAIERGVKD
                     FLNQVRS"
     gene            complement(693..1172)
                     /locus_tag="VFY96_17965"
     CDS             complement(693..1172)
                     /locus_tag="VFY96_17965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608413.1"
                     /translation="MDLTKVLPSFDRIVGSLARVELYTYGSSVPEASLIEVACWMTDN
                     GLAAFFGLAGSNEGLDPLMNEVKPEDWDAEDERLIVSFADALRVCWIYQCSFMLRRGN
                     EYKTIWDSRAFVQHFDALMHLRRGWACAGENELSVYQNSLELSLVTACIAFKRDTSK"
     gene            complement(2279..3337)
                     /locus_tag="VFY96_17970"
     CDS             complement(2279..3337)
                     /locus_tag="VFY96_17970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608414.1"
                     /translation="MTDEIEKLERVIAHRLGRLKEAVTAGESIVVEESHRAAQHIDNL
                     EAEITILRDKLKEAEEVIDRKNLSQQQTEENLSAMIKNLQSDVKSREEVLANRENEII
                     NYKSTLEQNTKKMSELEVAHMKAQQELADQTRRAEELRKNSQNKIAALEAQLIQTEDL
                     ARQKTLATKQLQQELAAKPQEFEATLKSKDESLAQRDSEIADLKAQLKRLTKGISDVS
                     LLFKEAEAMTGVETHKTVASGNEPPPEIADPAISSRPQNDEKITTILPDALFETVSAE
                     TFQRIISELGQATNVIGPLASLMVHQQAKALGESVEKFPRARLPELLEALAKEISDEN
                     LQMYFLQHFTQNERMTLN"
     gene            3497..3934
                     /locus_tag="VFY96_17975"
     CDS             3497..3934
                     /locus_tag="VFY96_17975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608415.1"
                     /translation="MDDQTLVVTGKAVYKARRKHRAFTYRVMQIGLRDGSDRKGYIRI
                     EKGCQVRNWSFLERWKPDLNVTSVDYWALPLNKSPVFGDSHDVRVPSVYGGCRLSLGS
                     ATGNKRVDYAKGAADCNRLASATLSKLSVSCGAGDMGGFALTA"
     gene            complement(4441..4884)
                     /locus_tag="VFY96_17980"
     CDS             complement(4441..4884)
                     /locus_tag="VFY96_17980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608416.1"
                     /translation="MKISTKLALLSAVCLIFFSREAAPQEQQWLFYAPALSRLQGKLS
                     KVMKYGRPTYGEHPEQDEKIDVPILILQLPVRVKSPTSSSVNTQTTNVSFVQLIFPTE
                     SGSYSKHVNVNIVVEGTLAQGIKGEHFTDIVMTVKTVNPAKPPVY"
BASE COUNT         1241 a         1368 c         1377 g         1444 t
ORIGIN      
        1 ttgaattgat cgtcgacgct gatgagtttg tcgttcgatg cggcgatccg aatccgccgt
       61 acacgggcat tctcaccctg cattattcac cccagaggat cacgattctc gatcgtgaag
      121 gtcaggcgcg gcgtcgacag atgaagccgt actttagatt cgacagtttg cctgaaattc
      181 tccgagccgc aggcaggtac gttgacagca gacgcggggg agatccgtat ggtcacaaac
      241 tgttctgcat cggaagcggg catacagctt gaatatcaaa ccagagtggg aataaaatcc
      301 gagacgctat ccacggctgc gatccgcgaa atcgccgttc acatgttcaa acggcgaact
      361 cgaatctcaa acccgattga tatgctaacc cgccgaaggg agaggccgcc ttcttgatgg
      421 ctgctgacaa ggccgtttca aatccgcaat ctcaactgcg tacctggttt agaaaatctt
      481 tgacgccacg ctcaatcgct cgtcgtctcc gtcgcgactt gaacaggtta accatgccga
      541 tcactgctaa accaatgccg accgtgcaaa agggaagacc tatggtaagt gcgatttcgt
      601 cgagcattgt ctctcctgaa ccgatcgcaa aacaatcaag agataagcta tcttcttaga
      661 tcagctctgt gcttgcaatc atcccggcaa atctatttag aagtgtcgcg cttgaacgcg
      721 atacatgcag tgacgagact cagctcgagt gaattttggt aaacagataa ttcattttcg
      781 ccggcgcatg cccatccccg acgcaggtgc ataagtgcgt cgaaatgctg cacgaatgca
      841 cgcgagtccc agatggtttt gtactcatta ccgcgacgaa gcatgaacga acactggtat
      901 atccagcaaa cccggagcgc atctgcaaaa gatacaatga ggcgctcgtc ttcggcgtcc
      961 cagtcctctg gttttacttc attcatgagg gggtccagcc cttcattgga tccggcaagg
     1021 ccaaaaaacg ccgctaagcc gttatcggtc atccagcaag caacttcgat aagagaagcc
     1081 tcgggaacag aactcccgta ggtatatagc tcgaccctgg cgagtgaacc gacgatgcgg
     1141 tcaaacgaag gaagcacttt cgtaagatcc atatcggaat cgtgcgggtg tctcagagac
     1201 tgatacatat ctgaagctga tcacatttat tcgttggatg aaagtggcgc gcattgcaaa
     1261 tgttggacta ctttcatttc ttcgtcgaga gcccgcgtcc atccgacgct gccaatagta
     1321 gcgaatcgaa accgtctgcc atcacgttct cccgcccatt tttctgcttc ttgctgcgtt
     1381 tgaaacactc gcagttgaag tatgcgcgtt tgtccaatct gctcgccaat cagctggcgt
     1441 ctggcgccac cttttgtgtg agagggtcgt ctatcgaatc tgcgccgtgt gcttggcgat
     1501 ttcttcgttg ctttcacatc gttcgaagcg gttgcaatag gttgcgcttt tgatcgggtc
     1561 tccaacggct cgatgccgtg cgcctcgtta cgaatctgca gcaccggtag cacggctctc
     1621 aagcgatgtc gctcataccc gagtatccag tggaataacc aattcatctc cgtttggtcc
     1681 tcggtgaata cgattggttc gccatgttta ttaaccacaa aagccatgga taaggttttt
     1741 acgtagacga atcccggtgt ctcgtttaac ggtttgtcca taattgcgcc ccgctcaaat
     1801 ggtcagccag ctgaagcaat tggggtgcct gcacgcattt gaacactaaa tcccgatttt
     1861 ctgcagaatt gaggaaacag aaatgcggtt ttcggccaat tctggcaagc cagtgacatt
     1921 ttgacatgtc acgaatagag atgagtgata aaagcttttt ttggcatagc cgcgagagta
     1981 ggattagata acaaacataa tccacagctc gcgccagccg cgctacacgt ctctaccgcg
     2041 gagttctagc catcagcccg gactctatgg cgcagcggac gcctggcctg gttctgctcg
     2101 ggcttttaca ctggcgagca cagtcggtcg ctgtttaaac gtaggattac ggcgaaagtg
     2161 gctatgcaga ggcgagaccg tgcgagaagg acccgaacag ctctttcgct tgcggctatt
     2221 ccgaagaaag atgcaaggac tggacgcacg atcttcagat gtggcgagcc gctgtggttt
     2281 agttcagagt cattcgctcg ttctgagtga agtgctggag aaaatacatc tgcaggtttt
     2341 cgtctgagat ctccttagca agagcctcaa gaagttccgg cagtcgagcc cgaggaaact
     2401 tctcaaccga ttccccgaga gccttcgctt gttggtgaac cattaatgac gcaaggggac
     2461 caatgacatt agttgcctga ccaagctcgc tgattattct ctgaaacgtt tcggcagaga
     2521 ccgtctcaaa cagtgcatcg ggtaaaatcg tcgtgatttt ttcatcgttc tggggtcggc
     2581 ttgaaatcgc tggatccgct atttcgggcg ggggctcgtt cccactagct acggtcttat
     2641 gtgtttcgac tcccgtcatt gcttcagctt ctttgaacag caaagaaaca tcactgatgc
     2701 ccttcgtgag ccgttttaac tgtgctttga gatcagctat ctcggaatct cgctgtgcta
     2761 aactctcgtc tttgctcttc agtgtggctt cgaactcctg cggcttggcc gccaattcct
     2821 gttgcagctg tttggtagcc aatgttttct gacgcgcaag atcctccgtc tgtatcagct
     2881 gcgcttcgag tgccgctatc ttattttggg aattcttccg aagttcctcg gcgcgtctgg
     2941 tctggtcagc aagctcctgt tgtgctttca tatgagctac ttccaactcg ctcatttttt
     3001 tggtgttctg ctccaacgtc gatttgtagt taattatttc attctcgcgg ttcgccagaa
     3061 cttcctctct gctttttaca tcgctttgca agtttttaat cattgcagaa agattttctt
     3121 cggtttgttg ttgggaaaga ttttttcggt cgatgacttc ttccgcctct ttaagcttgt
     3181 ctctgaggat cgtgatctct gcttccagat tgtcgatatg ctgcgcggcc cgatgtgatt
     3241 cctcgacgac aatactttcg cctgctgtca ctgcttcctt tagcctaccg agtcgatggg
     3301 caatgactct ttcgagcttt tcgatttcgt ccgtcaaacc cgcagcattg ccgtttttta
     3361 gccgaaccgc cggcatggtc tcgttttttc tccagggcaa agtctcctgc atttttaact
     3421 ttaaagagtt gtagaaatcg ctcatgacat cctccgattg tctaacgact tgtcattgcc
     3481 atggagaata cacgcggtgg atgaccagac cctcgtggtg acagggaaag cagtatacaa
     3541 agcccggaga aagcatcgcg ctttcaccta tcgagtgatg cagatcggcc taagggatgg
     3601 cagtgatcga aaggggtata ttcggattga aaaaggatgt caagtcagaa attggagttt
     3661 tttggagcgc tggaagccag atctcaatgt gacgtcggtc gactattggg cgctgcccct
     3721 aaataaaagc cccgtgtttg gtgatagtca tgatgttcgg gtcccgagtg tttatggcgg
     3781 ctgccggttg tctttgggtt cggcgactgg aaacaaaaga gttgattacg cgaaaggcgc
     3841 agcagactgt aatcgtttgg cgtctgcgac tttgtcaaaa ctcagcgtaa gctgtggtgc
     3901 tggggacatg ggaggttttg ccttgacagc gtaagttgtt gagtttacgt aggcatcgat
     3961 gtcccttcga gccgcgttct cgagaaatcg cttgttcctg tcttccgcag cgcttatttg
     4021 gtgttcgacc tggcgctttt gtgcttccgg atcgaaggca tcttttacaa gcgcatattg
     4081 gattcccgtg tcataccgta cccgcatatg gttcctccgg tcgaataatg gccgatgaaa
     4141 cgcaataaac aatccagctg taaattgacg aaatcccgag gaacttagtg tgacaggtgc
     4201 ccgtcaattt gtccatgttg agacataact gacattaaat ttggttgacc gaaaatatcg
     4261 cagccaactg gccgggcgct tggaggcttg actgcgcttg cggcaccacc gagcggcaac
     4321 gagttgccgg gcacgatccc attaaagatt taagatggaa aggaaaacaa gttcgctcgc
     4381 agctaaaatc tctcatccat cagtgtcttc cgcatggact aatttcagca ggatcctgga
     4441 ctaatacacc ggaggttttg ccgggttgac cgtttttaca gtcatcacga tgtcggtaaa
     4501 atgctctcct ttgatacctt gggccagcgt gccctcgacg acaatattca cgttaacgtg
     4561 ctttgagtac gaaccgctct ctgtggggaa gatcagttgt acaaatgaca cgttggtggt
     4621 ctgcgtgttc acgctgctcg atgtggggga tttaacccgg accggcaatt ggagaatcaa
     4681 aatggggacg tctatcttct cgtcctgttc tgggtgctca ccgtaggtcg gcctgccata
     4741 tttcatgact ttgctaagtt tgccctggag cctcgacagc gcaggagcgt agaataacca
     4801 ttgctgctct tgaggcgccg cttcgcgaga gaaaaaaatc aggcaaactg ccgagagcag
     4861 cgccagctta gttgaaattt tcataacggt cgacgaccgg cgctcgccgt cagaaatcgt
     4921 tcttgctaca atgagtgcgg ggccgagtca cattgtgact tctgcgtgct cacgaagctt
     4981 tcctcggaca tcgtagtgtc ggcattctac ttcacggttt cggtctactc gcagagcaat
     5041 ccgcgcacaa acgatcagaa ttaggcaaat gctctaattt tagcttcggc acttgaattg
     5101 cggcgctcgg gtcgtcacat gcccagcgac gatagcgaag tcgctttggg ccttcatcgt
     5161 caataaaact cggttggtca gtcataatga gaaaaaatct taggcctcaa atctgtccac
     5221 ggtgcgacgg acgaaacatt cgccggtcgc accgaaaaaa tctaacggaa aaggcgcgca
     5281 gcctattttt cgttaggccc taccgatgcg tggattgtca ttatcgtttc tggggatatt
     5341 gggtgccgtg gcggctttct tttttgacaa tgccacttcg ccagcacttc gccaatccga
     5401 acaagataag tagttcccgc gataactaag
//
LOCUS       DASULZ010000872         3654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_303265, whole genome shotgun sequence.
ACCESSION   DASULZ010000872 DASULZ010000000
VERSION     DASULZ010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303265"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VFY96_17985"
     CDS             complement(<1..453)
                     /locus_tag="VFY96_17985"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5608417.1"
                     /translation="MYRKVLVPLDESDLSASVLPYVRWLSSALKTPVELLHVEDVNRL
                     APYSPPLQIGYLETIAESFSQGSGVKCTIEHGSPAELIIHKASAEPGVLVAMATHGYT
                     GAARWLLGSVTQKALRGLHSDLLTVRPGGESDTGHAELKTMIVPLDLSP"
     gene            679..1557
                     /locus_tag="VFY96_17990"
     CDS             679..1557
                     /locus_tag="VFY96_17990"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX5608418.1"
                     /translation="MTLAVNEYQLRDIYAQSLQNLQEDAMEVAISSLLFKSHRLDEVI
                     DLSREHGVGWLEVWSEHLWRDDKGDLVKRLRNSGLSLSVHGPISDLNITSSNPGIRQE
                     SMRQVLQGIEESAAMGARVITIHPGYLTGRQDGPEGIWDMQISAFTRFASRGKELGLA
                     IAMETMEKRNKEVVIHPEVVNQIIDAVNMDNFGVTFDISHAHTVMDVVEFIRALRKIS
                     HIHISDTKQGKVHVLMGEGEIAFGPVLRALKEKYDGALVIEGWNPEDELGMVQRSTAF
                     LTTQLGKLTTNLNNEV"
     gene            1559..2563
                     /locus_tag="VFY96_17995"
     CDS             1559..2563
                     /locus_tag="VFY96_17995"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5608419.1"
                     /translation="MDKLFRRSLWLGIAANALVLLNVNLIFGQSKSSEVLLYTSVPQA
                     LATQFADAFMKKRPDIKVEIYRAGSTEVGAKLAAEREVGGIRADLLWLADAPVYYDLR
                     KRGELLGYISPEAKAIPAELKDPKGFFTAGRLINMIIAVNTQVTPHKDAPKSWKDFPD
                     FGKKAAMGNPLYSGSNFVTVAAFVKKDGNWSWFERARAKGVAIVRGNSEAADALAGKE
                     FGIAMTLDYIIAGLAKKGAPLATIWPTDGAISVPSPIAIIKNSKNPTGSKAFVDYVLS
                     KEGQEFLVKQEVIPVRSDVAAPKGQPSASQIKFMPIPYEWAAENAAEIRERFEKIMLH
                     "
     gene            2718..>3654
                     /locus_tag="VFY96_18000"
     CDS             2718..>3654
                     /locus_tag="VFY96_18000"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX5608420.1"
                     /translation="MSARRFDRRYLLMIAIGLFIFLAVIYPLWQVFLRSFISAGLFSL
                     KNYYKVFSFPSYYHALFNSLWVSAVTGVLCMLLGTVLAFLVIRTDLPFKKSLRTLLVL
                     PYALPSFFAAIAWIQLLGPQGYLARIFFRLSGSDTAPWNIYSAAGIVFVLTVHYFILV
                     FITVAGALERMDASLEEAARASGAKTYQIMRQITLPLVLPAILAGGLLAFIGALANFG
                     IPALLGMRARFFVLTTSIYYALAIPDFGLATALSSMLVLVALISMSFQFGIQKGGSRY
                     TVISGKAVHPSELKLRTMRSPLFFATAALVFFISII"
BASE COUNT          836 a          974 c          986 g          858 t
ORIGIN      
        1 gggcgagaga tccaacggca cgatcatcgt tttcagctcc gcgtgcccgg tatcactctc
       61 tcctcccggc cgcaccgtca acaagtcgct gtgaagccca cgcaaagctt tctgggttac
      121 gctgccgaga agccagcgcg cggctccggt gtagccatgg gtcgccatcg caaccagcac
      181 tcccggctca gcacttgcct tgtgaatgat caattctgcc ggactaccgt gctcgattgt
      241 gcatttcacg cccgagccct gagagaacga ttcggcaata gtttccaagt agccaatttg
      301 cagtggcggc gaatagggtg ccagacggtt cacgtcttca acgtgcagca actcgaccgg
      361 cgttttcagg gcagaggaca gccagcggac gtatggtagg acgctggcgg atagatctga
      421 ttcgtccaga ggtactagta cttttctata catggctctc ccaccttata gtttcttttg
      481 tgacacgcga aaatagccag aaccatgcct gaaacggttc ccgataatct cgttgagtaa
      541 cgtctattac cgccggtaga aatctcaaga actctaaact agccgacgaa aaactcgcaa
      601 ctctgcgaaa acaggatcat accgatcgca attttgaaaa cagctaacca gccgctgaaa
      661 tctcacggag atgctgtctt gactttggcc gtgaacgaat atcagcttcg tgatatttac
      721 gctcagtcgc ttcaaaacct ccaggaggat gccatggaag ttgcgatttc cagcctgctt
      781 ttcaagtccc accgactcga tgaggtaatc gacttgtcgc gcgagcacgg cgtgggctgg
      841 ctggaagtct ggtccgagca tctgtggcgg gatgacaaag gggatctggt gaaacggctg
      901 cgtaacagcg gtctcagctt gtctgtgcat gggccgatca gcgacctcaa tatcacttcc
      961 agcaacccgg gaatacgaca ggaatcgatg cgtcaggttc ttcagggaat tgaagagagc
     1021 gctgcaatgg gcgcccgggt gatcaccatc catcctggct atcttacggg ccggcaggac
     1081 ggccctgaag gaatctggga catgcaaatc agcgcgttca cgcgctttgc gagcagagga
     1141 aaagaactgg gcctcgctat tgctatggag acgatggaaa agcgcaacaa agaagtggta
     1201 attcatccgg aagtcgtcaa tcagatcatc gatgccgtga acatggacaa cttcggtgtg
     1261 actttcgaca tctctcacgc ccataccgtc atggacgtgg tcgaatttat tcgggcgctc
     1321 cgcaagatca gccatattca tatcagcgat accaaacagg gtaaggttca cgtgcttatg
     1381 ggagaaggag aaattgcatt cggtcccgtg cttcgagcac tgaaagaaaa atacgatggc
     1441 gcgctggtga tcgaaggatg gaaccccgag gacgaattgg gaatggtgca aagatcgacg
     1501 gctttcctaa ctacgcaact aggaaaactg acaacaaatt taaacaacga ggtgtgatat
     1561 ggacaagctt tttcgtagat cgctatggct agggattgct gccaatgcgt tagtgctcct
     1621 aaacgtcaac ctcatcttcg gccagagtaa gtcgagcgaa gtattgctct atacttcagt
     1681 gccccaggcg ctcgccacgc aattcgccga tgctttcatg aagaaacggc cggacatcaa
     1741 agtggagatc tatcgcgccg gcagtactga agtcggtgcg aagctcgcgg ccgagcgcga
     1801 agtcggcggt attcgagcgg atttgctctg gctcgccgac gcgccggtct attatgacct
     1861 gaggaagcgg ggcgagttgc tcggctacat ttcacccgag gcgaaggcga ttcccgccga
     1921 gctcaaagat cccaagggat ttttcaccgc cgggcgtctg atcaacatga ttatcgcggt
     1981 gaatacgcaa gtgacgcccc acaaagatgc gcccaaatcg tggaaagatt ttcccgattt
     2041 cggcaagaaa gccgccatgg gcaacccgct ctattccgga tcgaactttg tgaccgtcgc
     2101 cgcctttgta aaaaaagacg gtaactggtc gtggttcgaa cgcgcgcgag ccaaaggcgt
     2161 ggccatcgtg cgaggaaact ccgaagccgc ggatgcattg gcgggcaaag agttcggcat
     2221 cgccatgacc cttgactata tcatcgctgg attggcgaaa aagggcgcac cgctggcaac
     2281 catttggccg acggatgggg ccatttcggt gccgagtccc attgcgatta tcaagaacag
     2341 caaaaatccg acgggtagca aagccttcgt cgactacgtg ctctcgaaag aggggcagga
     2401 gtttttagtc aagcaggaag tcataccggt ccgcagcgac gtcgcggcgc cgaaaggaca
     2461 accttccgcg agccagatca agttcatgcc gatcccctac gagtgggccg cggaaaatgc
     2521 cgctgagatt cgcgagaggt ttgagaagat catgcttcac tagggcgtta cttacagcga
     2581 gttgtgtcac aacgacaaac ttcaggcgat tgtctttctg cgcgcgagcg aagaatcttg
     2641 tgtggtaaaa aagatccttc ggctaatgcc taggatgaca acacgaagca attaaagcga
     2701 atctcaagat catgcggatg agcgcccggc gattcgaccg gcgctatctc ctaatgatag
     2761 cgatcggtct atttattttc ctcgccgtta tttatccgct ttggcaggtt tttctgagga
     2821 gtttcatcag tgccggtctc ttctccctga aaaactacta caaagttttt tccttccctt
     2881 cgtattatca cgcgcttttc aattccttgt gggtgtcggc agtcaccggc gtgctctgca
     2941 tgctattggg aacagtattg gcctttttgg tgatccgcac cgacctgccg ttcaaaaaaa
     3001 gtctccgaac gctgttggtt ttgccttatg cgctgccgtc attttttgcc gccatcgcct
     3061 ggattcaact tctcggacct cagggctact tggcaaggat tttttttcgc ctttccggtt
     3121 ccgatacggc accgtggaat atctacagcg ccgctgggat tgtctttgtg ctgacggttc
     3181 actatttcat tctggtcttc attaccgtag cgggagctct ggagcgcatg gatgcgagcc
     3241 tcgaagaagc ggcgcgcgct tcgggagcga agacttacca gatcatgaga caaatcacgt
     3301 tgcccttggt tctgcctgca atcttggcgg gaggactgct cgcgtttatc ggcgcgctgg
     3361 cgaatttcgg cattcccgcg ctgctcggca tgcgcgcgcg cttctttgtc cttaccacga
     3421 gtatttatta cgccttggcg attccggatt tcggtctcgc gaccgccctt tcgagcatgc
     3481 tcgtgctggt cgcgctgatc tcgatgtcct tccagttcgg gatacagaaa ggcggcagca
     3541 gatacactgt catttccggt aaagcagtcc atccctcgga actaaagctg cgcacgatgc
     3601 gctccccgct tttttttgcc accgcggctt tggttttttt catctcgatc atcc
//
LOCUS       DASULZ010000873         4499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1217416, whole genome shotgun sequence.
ACCESSION   DASULZ010000873 DASULZ010000000
VERSION     DASULZ010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217416"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..558)
                     /locus_tag="VFY96_18005"
     CDS             complement(169..558)
                     /locus_tag="VFY96_18005"
                     /inference="COORDINATES: protein motif:HMM:NF042761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HEX5608421.1"
                     /translation="MKTIVISFLTGIIFALGLGIGGMTQPAKVVGFLDFGGNWDPSLA
                     FVMIGAIGIYSFSYWVLRKGFSFASTSSLMAPTKIDARLIGGSALFGVGWGIAGICPG
                     PAITALASQNRSFWIFFYSHDRGDRGF"
     gene            complement(555..986)
                     /locus_tag="VFY96_18010"
     CDS             complement(555..986)
                     /locus_tag="VFY96_18010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEX5608422.1"
                     /translation="MENFTPISSLIGGILIGLSGSALLLFNGRIAGISGILGGVVSAK
                     KHDALWRAAFVAGLLTGGLILRIVTPGAFQFGIDRSAAALVIAGFIVGLGSRIANGCT
                     SGHGVCGISRFSPRSIVATTIFIIVGAAAVYVINHFLGGAV"
     gene            complement(1112..1429)
                     /locus_tag="VFY96_18015"
     CDS             complement(1112..1429)
                     /locus_tag="VFY96_18015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX5608423.1"
                     /translation="MHITSWLSAFDGLSRAGALLVQDRFYEWHWEMHPMWWWGWGIGM
                     MAMMFLFWVVFIIGLIVAIRWLLGKGRERPPDSALEILRQRYARGEINKEEFEARRED
                     LAG"
     gene            complement(1448..1867)
                     /locus_tag="VFY96_18020"
     CDS             complement(1448..1867)
                     /locus_tag="VFY96_18020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608424.1"
                     /translation="MKTFITAVFLVGAIGLGPLLAQEKKDMPVKEGMPMKGEKMQGGM
                     IMGKMKEMQEKMAEMHKGMDGMMEGQGMMKSDEMKNMGGMMGNMSGMMGDMSQIMGEG
                     KMTPEEMQNMSKMMGDMSSTMKQMSERMGRGSTKKTK"
     gene            complement(1894..2421)
                     /locus_tag="VFY96_18025"
     CDS             complement(1894..2421)
                     /locus_tag="VFY96_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003164953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5608425.1"
                     /translation="MLSESIYSDIGSTYLHHYEASAIVCALPEEVFDHLDDHARLAAH
                     MSESSWMMGGGAMQIDVDAAKGQRTGSCIRLSGKIFGLTLFVEEIVTERTPPRRKVWE
                     TIGTPKLLVIGRYRMGFEVVQHEAGSRLTVFIDYALPHGVVTSWLGYLLGGYYVRWCA
                     QRMVGDAVRQFGSKP"
     gene            complement(2424..2684)
                     /locus_tag="VFY96_18030"
     CDS             complement(2424..2684)
                     /locus_tag="VFY96_18030"
                     /inference="COORDINATES: protein motif:HMM:NF039498.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5676 family membrane protein"
                     /protein_id="HEX5608426.1"
                     /translation="MKLDVRGFSIAVGSVIAAAFSVCAFFVAVAPEATAAIIGYLLHI
                     NLSGLTRTLTWPSYVAGMLAIGIWTGLWAAVVAKLYNMLTLR"
     gene            complement(2910..3314)
                     /locus_tag="VFY96_18035"
     CDS             complement(2910..3314)
                     /locus_tag="VFY96_18035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608427.1"
                     /translation="MKSRIVVMGLIFSGLLTIGQSFGAASTDSSQTVSSDGVTVKVTY
                     LARTEHETRFSVVMDTHSGNLDVYDLKANSILRDNTGLAMQPTGMENKGSGHHRQTIL
                     TFPRPSTRAKWVELVINNLAGEKERVFRWDVK"
     gene            complement(3999..>4499)
                     /locus_tag="VFY96_18040"
     CDS             complement(3999..>4499)
                     /locus_tag="VFY96_18040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608428.1"
                     /translation="YGWLSVATFFVQILLTSQTLKKLGLAASLYVTPAALLTGSIVII
                     TWPGLPAATFTRMADAILRNSVHRSGMEVIYMAVPVTVMKAIKTFLDVVVERIGDASA
                     GFIILLFSLFATEKYIAYVHFICLGLIFTWFGFNRFLQKECPQAVRKRMPFEETSPRT
                     GWLGCD"
BASE COUNT         1150 a         1332 c          986 g         1031 t
ORIGIN      
        1 gccgctacgc aattttatcc agccgataaa ccctgtttcc tttgtcacca ttttcgtctc
       61 cagctacggc caatcgccaa gctaaaacaa accatctaca tatcctgaac cacttcacgc
      121 ggcgaattcg agcacgccgc ccgatgcagc aataaggcat ttttcaaatc aaaagccacg
      181 atccccgcga tcatggctat aaaaaaaaat ccagaatgag cggttttgcg aagcaagcgc
      241 cgtaatcgcc ggaccggggc agatgcctgc tatcccccag ccaactccga agagcgcaga
      301 tcctccgatc aggcgcgcgt cgattttcgt cggagccatc aaagaactgg ttgatgcaaa
      361 cgaaaaaccc ttccgcaaca cccaatacga gaaagagtaa attccgattg cgccgatcat
      421 tacgaatgcc agactcggat cccagttacc cccaaagtcc aaaaagccga cgaccttcgc
      481 cggctgcgtc atgcccccga taccgaggcc caaggcaaat atgattcccg taagaaacga
      541 aataacaatt gtcttcatac ggcgcctccg agaaaatgat taatgacgta gactgccgcc
      601 gcaccgacga tgataaaaat cgtcgtcgca acgatcgacc ggggcgagaa tcggctaata
      661 ccgcagacac catgaccgct ggtgcagcca ttggcgatgc gcgaaccaag gccgactatg
      721 aagcctgcaa tcacgagggc cgccgccgaa cgatcaattc caaattgaaa agcgccagga
      781 gttacaatcc ggaggatcaa cccacccgta aggagcccgg cgacaaacgc agcgcgccac
      841 aaggcatcat gcttttttgc gcttactacc ccacccagta tcccgctgat tcctgcaatc
      901 ctgccgttga acagcagcaa ggctgatccg ctcaagccga taaggattcc gccgatcagg
      961 gacgaaattg gtgtgaaatt ttccattcaa tctccaactt tattctgcgt actgtgccgg
     1021 agcctttttt gcagcgcatc aaagccgcca accaacagca tcgccgagac aattgccgta
     1081 gtaaaacttc ttcatcgcac cactgctttc ttcatccggc gagatcttct ctcctggcct
     1141 cgaactcctc tttgttaatt tctccgcgag catacctttg acgcagaatt tcgagtgccg
     1201 aatccggggg tcgttcccgg cctttgccga gaagccaccg gatggcaacg atgagaccaa
     1261 tgatgaaaac cacccagaag agaaacatca tcgccatcat cccgataccc cacccccacc
     1321 accacattgg atgcatttcc cagtgccact cgtaaaaccg atcctggacg agtagcgcac
     1381 ctgctcttga gagaccatcg aacgcactca accaacttgt aatgtgcatg attgcctccg
     1441 taattcgcta tttggtcttc ttcgtacttc cgcgccccat ccgctcggac atctgcttca
     1501 tcgtggacga catgtctccc atcatcttcg acatattttg catttcctcc ggcgtcattt
     1561 taccctctcc cattatttgg ctcatgtcgc ccatcattcc ggacatattg cccatcattc
     1621 cacccatgtt cttcatctca tcgcttttca tcattccctg gccctccatc atgccatcca
     1681 ttcccttatg catctccgcc attttttctt gcatctcttt catcttgccc attatcattc
     1741 cgccctgcat tttctcaccc ttcatgggca tgccctcttt caccggcatg tctttcttct
     1801 cctgagccag cagcggaccc aacccgatag cccctaccag aaatacggcc gtaataaacg
     1861 ttttcatgac gacctcctat tttccacgac aacctatggc ttcgatccga attgccttac
     1921 cgcatcgcct accatccgtt gcgcacacca ccggacgtag taaccaccaa gcaaataccc
     1981 aagccaactg gtcaccacgc cgtgcggcaa ggcgtaatcg ataaaaacgg ttagacgcga
     2041 accagcttca tgctggacga cttcgaaccc cattcggtag cgcccaatca ctagaagctt
     2101 aggtgttccg atggtctccc agacttttcg ccgtggcggc gttcgctcag ttacaatctc
     2161 ctcgacgaaa agcgtgaggc caaaaatctt gccgcttagc cggatgcacg agccggtgcg
     2221 ttgtccttta gcagcatcga catctatctg catcgcaccg ccgcccatca tccaggatga
     2281 ctcgctcatg tgagcagcta gccgggcgtg atcgtccaaa tggtcgaaaa cttcctccgg
     2341 taacgcgcac acaattgcgc tcgcctcata gtggtgtagg taggtcgagc cgatgtcgga
     2401 atagatgctt tcgctcagca cggtcacctc aatgtcaaca tgttgtagag ctttgctacg
     2461 acagcagccc atagacctgt ccatattccg atcgccaaca taccagcgac gtaacttggc
     2521 caagtaagag ttcgcgttaa ccccgacaga ttaatatgca agagatagcc aatgattgca
     2581 gcagtggctt ccggagcaac agcgacgaag aaagcacaaa cactgaaagc cgccgcgatc
     2641 acgctcccta ccgcaatgga aaatccacga acatcaagct tcatgacgta cctcctccag
     2701 caaaaatgct gacccgatgt tgatcgttca gctaatgctg atggctgtca tccgtgcgtt
     2761 tattatgccg atgagttctc gagccgcagc accctccacc ggagagcatc atccaaacaa
     2821 acagcccaac gaagacgacc cagatccagc ttccttgtaa gaattccatg ggctttctcc
     2881 attcggtcgg ggctctggtc agtacttgtt tacttaacat cccaacggaa gactctttct
     2941 ttctcccctg cgagattgtt gatcacaagc tccacccact tggcccgggt ggacggcctg
     3001 ggaaaagtga gaattgtttg ccggtgatga ccgcttccct tgttttccat tcccgtcggc
     3061 tgcatcgcca agccggtgtt atcacgcagt atgctgttgg ccttcagatc gtaaacgtcc
     3121 agattgcctg aatgggtatc catgaccact gaaaaccgcg tctcatgttc cgtccgtgct
     3181 aagtaagtca cctttacggt gacaccatcg ctggaaactg tttgcgatga atcagtgctc
     3241 gccgcgccga agctctggcc gatcgttagg aggccactga aaatcaatcc catgaccact
     3301 attctcgatt tcatgtttcg ttcccctcgc catcaaccta agaaacaccc atacgcgtag
     3361 ccaaaaagta cggctaccga gacaaagcca aaaggagttg agcaatcaga tgcgaagaac
     3421 ggaaagagag agataacgcg gcgaggaaat tggaaaagcc agggtggaga ctcgtctaaa
     3481 taacgcgaaa acccaaaggt tcacacttgc catgtccacg tcatcgtgca ggaccggtaa
     3541 gccgttcacg tgcgaatcga ttttgcctgt tatgctggct ggctgatttg gagctgatgc
     3601 tgaggctaga ggttcaatgc gatattcaga gccgatacat tggatgtttg ctttgagatg
     3661 atttagcaga gtagaagcga ggtaagcctc ggcgatcgag acggcggtct ccgtcccatt
     3721 ttcgtccgtg tgaaaacact ccaacaccgc ccatgcgaca ctatagtgca cgagcacacc
     3781 cgtgattagc gcaatgataa aaatccttag tcgtcgggaa cgcatcctgt caaatctgga
     3841 gagcacgaat tatgccgtaa gcattgagct catagcccgg cgatttggct gcaaactcat
     3901 tctccttcat gcaaattacc gcaccaggtt ttcgcattta tgcgaagatc ggacccttct
     3961 caaatccaag ctctctcgat gacaagatcc ataaattttc aatcacaccc cagccagcct
     4021 gttctgggcg aggtctcctc gaagggcatt ctcttacgta cagcttgcgg gcactctttt
     4081 tgtaaaaatc ggttgaatcc aaaccaggta aagatcaatc ccagacaaat aaaatggacg
     4141 taggcaatat atttttccgt tgcgaaaaga ctgaacaata gaataataaa tccggcactc
     4201 gcatcgccga tcctttcgac gacgacatct agaaaagtct tgatcgcctt catgaccgta
     4261 acgggtacag ccatgtagat cacttccata ccgcttcgat ggacgctgtt tcgtaagatt
     4321 gcgtctgcca tgcgtgtgaa tgtcgccgct ggaagaccag gccaagtgat gatcacaatg
     4381 gaaccggtaa gcagcgccgc gggggtcacg taaagacttg ctgccagccc tagctttttt
     4441 agggtctgac ttgtcagcaa gatttgaaca aaaaaggttg ccacactcaa ccagccata
//
LOCUS       DASULZ010000874         3093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_1217518, whole genome shotgun sequence.
ACCESSION   DASULZ010000874 DASULZ010000000
VERSION     DASULZ010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217518"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(350..1426)
                     /locus_tag="VFY96_18045"
     CDS             complement(350..1426)
                     /locus_tag="VFY96_18045"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5608429.1"
                     /translation="MAIFTGELGLQFVTHLANHYTVPDLVRLAQFAADKGFKQIWAND
                     NIRYRGQLVVLTAIAARVPIQLGTAIMVPYFHNPLDVADCLGALAELCEGREIGVGIA
                     RGDLGQSPQHVQLLKPLAMVSQTARFLRSALGGEEIVYSDYPFLQEFYRLNPSGKFRL
                     AFKPKAAFKFYGGGNGPQSLRMCGRVMDGLISSGTYIPMLKAGRLPGMLATADQAAKE
                     SDPNKRLRKVCELNVSIARDREKAIEFPKRQVAHSILQWEALGFTPEEYARMGVEREQ
                     VLRLKEAFASGALVEEAAKLVTEHMLKTYYAAGTPEEVRDQIIELVGAAGRFGYDHVA
                     FAKLGPDYEEAINLLADQVVPALR"
     gene            complement(1745..2626)
                     /locus_tag="VFY96_18050"
     CDS             complement(1745..2626)
                     /locus_tag="VFY96_18050"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX5608430.1"
                     /translation="MKEVKEFFENVSPETTTGHTISVRRTLRRGLLLGLLLSSLMLRS
                     ALAGGPTGWGIGSCREITAFPANLVDSGVYCLSYKYIDFPLMSGVLIKISANNVVLDL
                     NGATLDGTPKRNGAFSWGIQSHGHSNITVRNGTIRGFNYGISLTAPLGYGTPVRPSHS
                     FLVENVRLIENRTAGMEVIGYDSVIRGNYVAHTGGSPDTGLSGHAWGIIAAGDGLRLI
                     DNDVTHTHVNTAHSGGKTTAIFLSGGGNAAVVKNRMSEADFGIYSGGLNWGKYRGNIT
                     VNVTTPYTGGTDAGDNN"
BASE COUNT          652 a          848 c          860 g          733 t
ORIGIN      
        1 gggctcctta gaagaggacc actttctgcg gctgttccgc atcgtcgaac cgcgtcgatg
       61 aaactctccg ggtgctcata gagggccgta acgcttcttt gcttcaaata gtcaatacaa
      121 taccggcgac gatcgcgcga aaactgaaag tctttgatcg aagcgtttgg tttgacttta
      181 tgctacctta gatcgcgaaa ttatgcagtt gcgccgctcc gacccggagt tatgcttcta
      241 acgtagagaa gtaagcttca ctcttgtccc accattttgc ctgttttctt gaatgcaagc
      301 gcgtttcgtc gggagagcgc gcattcattt ttaaaaaaac ttgagaacgt tatcgcagcg
      361 ccggcacgac ttgatcggcg agcagattga tcgcttcctc gtaatcgggg ccgagcttgg
      421 cgaaggcaac gtgatcgtag ccgaagcggc ccgccgctcc gacgagttcg ataatctgat
      481 ctctgacttc ttccggtgtc cccgcggcat agtaagtttt gagcatatgt tcggttacga
      541 gtttggctgc ctcttcgact aacgctccgg atgcgaacgc ttctttgagc ctcaacactt
      601 gctcccgttc aactcccatc ctcgcgtact cttccggcgt aaagcccagc gcttcccatt
      661 gcaagatcga gtgagcgacc tggcgcttgg gaaattcgat cgctttttca cggtcgcgtg
      721 caatcgaaac attcagttca cagacttttc gcagacgctt gttggggtcc gactctttgg
      781 ccgcttgatc cgcagtcgcc aacattcccg gcagccgacc cgctttcagc atcggaatat
      841 aggtcccgga gctgatcaac ccatccatca cgcggccgca catgcgcaga gactgcgggc
      901 cgttgccgcc gccgtagaac ttgaatgctg ctttgggctt gaacgcgagg cggaattttc
      961 cgctcggatt gagacgataa aactcctgca ggaacgggta atcgctataa acaatttctt
     1021 ctccgccaag cgcgcttcgc aggaagcgcg ccgtttggct gaccatcgcg agcggcttca
     1081 gcaattgaac atgctggggc gattggccaa gatcgccgcg tgcgatgccc acaccaatct
     1141 cacggccttc acatagttcg gcaagtgcgc caaggcagtc cgcgacgtcc agcggattgt
     1201 gaaagtaagg caccatgatc gcggtgccga gctgaatcgg gacgcgagcc gcgatcgcgg
     1261 taagcacgac cagttgtccg cggtagcgga tgttgtcgtt ggcccagatt tgtttgaagc
     1321 ctttgtcggc cgcgaattgg gccaatcgga ccagatcagg caccgtgtaa tggtttgcca
     1381 ggtgtgtgac gaattgaagt ccaagttcgc cggtgaaaat cgccattggg gtccctttag
     1441 atttccgtaa tttgttttgt cttgagtttc ttcgttatcc gttccggtat caggcttacg
     1501 cggtattctg ccgggctcaa gatactgttt cgatccgcat cgctttggaa gatcagttgg
     1561 cgctgattgc ttcgacgatc acgtggtaaa acaccccagc atattccgcc ccggtgttcg
     1621 cgccatgtcc gcccgatact cgagcttagg tcgttaagtc aataggaagc ctgacactaa
     1681 gtcccctctt gccggaaaga ttgccccggc gaatgtgtac aaccagaagg gagtgctttc
     1741 ccgctcagtt gttgtcgccg gcgtcggtgc cgccggtgta aggcgtagtg acgttgactg
     1801 tgatattgcc gcggtacttg ccccaattga gccctccgct gtagattccg aagtcggctt
     1861 cgctcatgcg attcttgacg actgcggcgt ttccaccccc ggatagaaaa atggccgtgg
     1921 ttttgccgcc gctgtgggcg gtgttgacgt gcgtgtgcgt cacgtcatta tcaatgagcc
     1981 ggagcccgtc gccagctgcg atgattcccc aggcgtggcc tgagagccca gtgtccggag
     2041 agccgcccgt atgagcgaca taattgcctc gaatcacgct atcgtagcca ataacctcca
     2101 ttcccgcggt ccggttctcg atgagccgga cattctccac caaaaaactg tggctcggcc
     2161 gcacgggcgt gccgtatccc agcggagcgg tgagcgagat gccgtagttg aatcctctga
     2221 tggtgccgtt gcgcacagtg atgttggagt ggccgtgcga ctgaatgccc caggagaacg
     2281 ccccgttgcg cttcggagtc ccatcgagag tggcaccgtt caggtcgaga acgacgttgt
     2341 tcgccgagat tttaatgaga acgcctgaca taagaggaaa atcgatatat ttgtagctca
     2401 ggcagtatac accggaatcc acgagattgg ccggaaaggc ggtaatttct cggcaagaac
     2461 cgattcccca gccagtggga ccgccagcca gcgcgctgcg cagcatcaag cttgataaca
     2521 gcaatccgag tagtaaacca cggcgcagag tgcgccggac gctgatcgtg tgtccggtcg
     2581 ttgtctccgg cgaaacgttt tcgaaaaatt cctttacttc tttcatgttt cctcctccgc
     2641 atattaagtg ctgaacacgc ggggcgcttc agaccgacga aactatatga gacgtgacag
     2701 agttaaggat taatggtgag ctttgggtgt ggcacaaatt ggttttgtac cataccttca
     2761 ggaaagatac atgtgatcag agcagtagct ggtcaattca gactctcgtg aactgtaaca
     2821 gcatttgagc gttcgccgtt cgtacttcga aaccctatca gcacgaacgg cttttccttg
     2881 tccgctcgcc ctgagcttgt cgaaggacgc ctctaggaga gagaaggggt gagggcgagc
     2941 tgcgcatcgt catcgactgt ccgattcccc cgagcaagcg cgaagcgcgt gtcgacagga
     3001 ttgaactttg aagtcgaaat gcggaacgtt gaaccgtccg aggcgctgaa ttagagtggg
     3061 gcgcatcatg cgtaccgcaa cacccccaga tcg
//
LOCUS       DASULZ010000875         4462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_303620, whole genome shotgun sequence.
ACCESSION   DASULZ010000875 DASULZ010000000
VERSION     DASULZ010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303620"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VFY96_18055"
     CDS             complement(<1..674)
                     /locus_tag="VFY96_18055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX5608431.1"
                     /translation="MLDQMKSWGARSTRAGILGVLTFFFAFPFVWMLITAFKTTSDLY
                     NLKHNPFLFYEWPTLEHVERLFFETLYGEWLINTAFVGILVVLITLALAVPAGYALAR
                     LSGSWGAKVGIGIFLTYLVPPTLLFIPLSRVVVNLHLDDSLWSLVLVYPSFTVPFSAW
                     LMMGFFKAIPRDVEEAAMVDGYSRLGAFFRVVVPISFSGILTVAIFSFTLVMQEFVYA
                     VTFISSV"
     gene            complement(678..1625)
                     /locus_tag="VFY96_18060"
     CDS             complement(678..1625)
                     /locus_tag="VFY96_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX5608432.1"
                     /translation="MNSSDSIATSAQSFSPRHRRGSAFLDNERWLGPLLIGPAVLYIV
                     LLVGVPFLMALYYSVSNTTTGGGTMGFVGLRNFTAVIGTPKFQTALKNTLTFGLISQA
                     LIIVLANALALALQTEFPGKRFIRFLILLPWVAPISIGTIAWLWIFDSTYSVLNWMLR
                     LAGLIHGTGYMWLGLPHLAMGSIIAVHIWRQLPLATVILLAGYGSIPQDIHDAAAVDG
                     AGFWRHFFQVTLPLILPITLVALLFGLIFTFTDMVIIYNLTRGGPFDTTQVLASLAFY
                     TGIAGGDLAEGAAIALFLFPLLLAVALGFLMIAHRTEVT"
     gene            complement(1767..2798)
                     /locus_tag="VFY96_18065"
     CDS             complement(1767..2798)
                     /locus_tag="VFY96_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5608433.1"
                     /translation="MATVETRDIKKIFGDIVAVNGVNLLTRDGEFLVLLGPSGCGKST
                     LLRMIAGFEKPTEGELLIGGAVMNELPPRARKIAMVFQSYALYPHMTVYKNIAFPLKA
                     QGMRKALIPQKVEWAASLFGIGHLLERKPRALSGGERQRVALARALVREPSVFLLDEP
                     LSNLDAQLRASARDELQQFQRRIGITTIYVTHDQVEAMGLGDRIAVMKAGSVRQIGTP
                     QEIYDEPADAFVAGFVGSPPMNLVRHQKYILGFRPEDFHPKGIHNGGARLATFKLNVT
                     RLEYLGADRLIYGTLVGDYSDQKVIARLPVTVPVKVEAANSYDFTVPEDELRFFDSST
                     ELRIPAQPI"
     gene            complement(2927..4270)
                     /locus_tag="VFY96_18070"
     CDS             complement(2927..4270)
                     /locus_tag="VFY96_18070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX5608434.1"
                     /translation="MAKRGLTRRDFIKTAAAGALTTGLAGPLILPGRAHGAAAKKTLK
                     ILQWVHFVPAFDKWFNETYIKQWGEKNNTEVTVDNIGLAGLSARAAAEVSAQKGHDLF
                     LFEWPPPFYEDQVIDMKDVYAECEKKYGKPIDLAIKSTYNPKTKKYFGFSPSFVPDPI
                     NWRKDLWDDVGVVPDSWEKILDGGRKIKQKHNIPVGLGLAQELDTSMALRTIMYSHGA
                     HEQDANGNLTLNSKNTVEAVKFVKALFQETMTPEVFTWDASSNNRAMLAGTISLALNA
                     ISITRTGEKDNPEFTEKIHLAKAAAGPVRRIGLEHVMGVYVIWKFAENIDGAKKFLID
                     YVANFRQAFVNGEFYDFPCFQRTVPDLKQLLAKDTKGKPAGKYSILEDVLNWATNIGY
                     PGYANAAIDEIYQKWIVPVMFAKAAQGVSSPEDAVKEAADESKRIWDKWRERKLI"
BASE COUNT         1038 a         1252 c         1247 g          925 t
ORIGIN      
        1 accgaggaga tgaaggtcac agcgtagaca aactcctgca tcaccaaagt gaagctgaaa
       61 atcgctaccg tcaggatacc cgagaaggaa atcggaacga cgactctaaa gaaagcgcca
      121 agccggctgt acccatccac catcgcagct tcttccacgt ccctgggaat tgccttgaaa
      181 aaacccatca tcagccaggc ggagaatggc acggtgaaag acggatacac caacaccagc
      241 gaccagagcg aatcgtcgag gtgaagattg accacgactc gtgagagtgg aatgaagagc
      301 aaggtcggtg gaaccaaata ggtgagaaaa ataccaatgc cgaccttggc gccccaactt
      361 cccgagagac gcgctaacgc gtagccggcg ggaaccgcaa gcgcgagggt gatcagcacg
      421 accaagatac cgacgaaggc tgtattgatc agccactcgc cgtacaaagt ttcgaaaaac
      481 agccgctcca catgttcgag agtcggccat tcatagaaaa gaaacgggtt gtgcttgagg
      541 ttatatagat cggacgtggt cttgaacgcg gtaataagca tccatacgaa tggaaacgcg
      601 aaaaagaacg tgagcacgcc aagaatgccc gcgcgagtcg atcgcgcgcc ccagcttttc
      661 atttgatcca gcatcgctta cgtgacctcc gtgcggtgcg caatcatgag aaagcccaac
      721 gccacggcga gcagcaaagg aaacagaaac aatgcgatgg ctgcgccttc tgccaaatcg
      781 ccgccggcga tgccggtata gaatgccaaa ctcgcgagca cctgagtcgt atcgaagggt
      841 ccgccgcgag tcaaattata gatgatgacc atatcggtaa atgtgaagat caagccaaac
      901 agcagcgcaa ccagggtgat cggtaagatc agcggcaaag tgacttgaaa aaaatgccgc
      961 cagaaacccg ctccatcgac ggcggcagcg tcgtgaatat cctgcggaat cgagccgtaa
     1021 ccagcgagca gaatgaccgt ggcgagcgga agctgccgcc aaatatgcac cgcgataatg
     1081 gagcccatcg ccaagtgtgg taagcccaac cacatgtaac ccgtgccatg aatcaacccg
     1141 gcgagcctga gcatccagtt gagcacgctg taagtcgagt cgaagatcca cagccacgca
     1201 atcgtcccaa tggagatcgg cgccacccac ggcaaaagga tcagaaagcg aatgaaacgc
     1261 ttgccgggaa attccgtctg cagcgcgagc gccagcgcgt tggcgagaac gatgatcaac
     1321 gcttgggaaa tcagcccgaa agtgagcgta tttttgagcg cggtttgaaa ttttggcgtg
     1381 ccaatgaccg cagtaaaatt tcgaaggcca acaaaaccca tggtgccgcc acccgtggtc
     1441 gtgttgctga cgctgtagta gagcgccatt agaaagggca cgcctacgag gagcacgatg
     1501 taaagcaccg ccggcccgat caacagcggc ccgagccagc gctcgttgtc gaggaaagca
     1561 gagccgcgcc gatggcgcgg cgaaaagctt tgcgccgaag ttgcgatcga atctgaagag
     1621 ttcatcagcg gttttcccgt ctccctatca atggactggc tcgcactggc gttcgtggtg
     1681 tttcgagcat cactgcgcta gtgcggggga ttgcttcggc tgcaccggtt aattcgtgca
     1741 ccatggctca cggccctagc gctggtctag atcggctgcg ccgggatgcg cagttccgtc
     1801 gagctatcga aaaaccgcag ctcatcttcc ggtaccgtga aatcgtagct attggccgcc
     1861 tccaccttaa ccgggaccgt aaccggcagc cgcgcgatca ctttctgatc ggagtaatcg
     1921 ccaaccaaag ttccataaat caaccgatca gcgccgaggt attcgagccg ggtcacattt
     1981 agcttgaagg tggcaagccg tgcaccgccg ttgtgaatcc ctttgggatg aaaatcctcc
     2041 ggccgaaaac caagaatata cttctgatgg cgcactaaat tcatgggcgg cgagccgaca
     2101 aagccagcga cgaacgcatc ggcaggctcg tcatagattt cctgcggggt gccgatctgg
     2161 cgcaccgagc cggctttcat caccgcgatg cgatcgccca atcccatcgc ttccacttga
     2221 tcgtgggtca cgtagatggt cgtgatgccg atgcggcgct gaaactgctg aagctcgtcc
     2281 cttgccgagg cgcgaagttg cgcgtcgaga ttggaaagcg gctcgtccag tagaaaaacc
     2341 gagggttccc gcaccagcgc ccgggcgagg gcgacgcgct ggcgttcgcc accggacagc
     2401 gcgcgcggct tgcgctcgag taaatgacca atgccaaaca gcgaagccgc ccattctact
     2461 ttctgcggaa tcagcgcttt gcgcattccc tgagccttca acggaaaggc gatgttcttg
     2521 tacaccgtca tgtgcgggta gagggcgtag ctctgaaaca ccatggcaat tttccgcgcg
     2581 cggggcggca gttcgttcat caccgcgccg ccgatcaaaa gctcgccctc ggtgggcttc
     2641 tcaaagcccg cgatcatgcg cagtaacgtg ctcttgccgc agcccgacgg accgagcagc
     2701 acgaggaatt ctccgtcgcg ggtaagtagg ttcacgccat ttaccgcgac gatgtcaccg
     2761 aaaatttttt tgatgtcgcg ggtttccact gtggccatag gtttatcgat tcacttatta
     2821 ccaagcctcg acaacaagcg ccactcaaag aaaaggagac gccgaactcg ggtctccttt
     2881 tctgctggcg gcaaatcgcc gcatgcctta tgggcgcggg cagagtttaa atcagcttgc
     2941 gctcccgcca cttatcccag atacgcttgg attcatcggc agcttccttg acggcatcct
     3001 cgggacttga cacgccttgc gctgccttgg cgaacatcac cgggacgatc cacttctgat
     3061 agatctcgtc gatggccgcg ttggcgtatc ccgggtaccc aatgttggtc gcccaattca
     3121 acacatcttc gaggatgctg tacttgccgg ccggtttgcc tttggtgtcc ttggccagca
     3181 actgcttcaa atccggcacg gtgcgttgga aacaggggaa gtcgtagaat tcgccgttga
     3241 cgaacgcctg gcggaaattg gccacgtagt cgatgagaaa tttttttgcg ccatcgatgt
     3301 tttcggcgaa cttccagatc acatagaccc ccatgacgtg ctcgaggccg atgcggcgca
     3361 ccggaccggc ggcagctttg gccaagtgaa ttttctcggt gaactccgga ttgtcttttt
     3421 ctcccgtgcg cgtgatcgag atcgcgttga gtgccaaaga gatggtgcca gcgagcatcg
     3481 ctcgattgtt cgaagacgca tcccaggtga aaacttccgg cgtcatggtc tcctgaaaga
     3541 gcgcctttac gaacttcacc gcttccaccg tgttctttga attgagcgtt agattgccat
     3601 tggcatcctg ctcatgggcg ccatgggagt acatgattgt gcgaagcgcc atcgaggtat
     3661 cgagctcctg cgctaagccg agccccacag gaatgttgtg tttttgcttg atcttgcgac
     3721 cgccgtcgag gatcttctcc cagctatcgg gcaccacgcc gacatcgtcc cataaatctt
     3781 tgcgccagtt gatagggtcg ggcacaaatg aaggggagaa gccgaaatat tttttggttt
     3841 tcggattgta agtgcttttg atcgcaagat cgatgggttt gccgtacttc ttttcacact
     3901 ccgcgtaaac gtctttcatg tcgatgacct gatcctcata gaagggcggc ggccattcga
     3961 agaggaagag atcgtgccct ttttgcgcgg atacttcggc tgcggctcgc gcgctcaaac
     4021 ccgccaaacc gatattgtcg acggttactt cggtgttgtt tttctcaccc cactgcttga
     4081 tataagtttc gttgaaccac ttatcgaacg cgggaacaaa gtgcacccac tgcagaattt
     4141 tcaaggtctt ttttgccgca gcgccgtgcg cgcgtccagg aagaatcaga ggtcctgcga
     4201 gcccggttgt aagcgctccc gctgccgcgg ttttgatgaa gtcccgtcga gtcaggcctc
     4261 gctttgccat gtctgcctcc ttgtgctcgg ttcgttttcg gcggtcggtg ccttgcgcgt
     4321 gagcttatcc agaattcagc tcatcaaatc ttgcacgggg aacataacgg agatggcggt
     4381 gccaagtcat ttggaagacg gggttccgca ggtaggcgac cgacttatgc gacgtgactc
     4441 catgcgtggt acgagctccg ca
//
LOCUS       DASULZ010000876         6607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_507241, whole genome shotgun sequence.
ACCESSION   DASULZ010000876 DASULZ010000000
VERSION     DASULZ010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 6607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6607
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507241"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1253
                     /gene="gcvPB"
                     /locus_tag="VFY96_18075"
     CDS             <1..1253
                     /gene="gcvPB"
                     /locus_tag="VFY96_18075"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX5608435.1"
                     /translation="GSCTMKYNPKINEVVARLPGFAEAHPLAPSELLQGALALLHELE
                     LILAEISGMEYVSLQPAAGAQGELTGLMLIRACLAERGDPRKKVIVPDTAHGTNPASS
                     ALCGYDVVQISSNERGVIDAVTVEKAMDDNVAAVMITNPNTLGLFETNIEAIAEIVHA
                     KGGMIYLDGANLNALMGIAKPGHMGVDVLHLNLHKTFSTPHGGGGPGAGPVAVTQALS
                     EFLPVPRIIKRGHRFELQEDCPKSVGRVRSFFGNFGILVRAYSYITALGADGLEDASR
                     MALLNANYIRKKLARNYQLAYNEPCMHECIFTDRLQQRNGVSTLDIAKRLLDYGHHPP
                     TIYFPLVVSGALMIEPTETETPETLDDFIEAMLAIAAEAKEDPELIKDAPYSTPVRRL
                     DEAKAARKPVLRWEPSSDADKTED"
     gene            complement(1303..1821)
                     /locus_tag="VFY96_18080"
     CDS             complement(1303..1821)
                     /locus_tag="VFY96_18080"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX5608436.1"
                     /translation="MPAAPELPPVTVDKPPENPRPAAPREQPLEETAIAERQLPTLKE
                     LLPSATWSSSSARADMPISLNTNDPVYVTYFTKIKQLIETQWQYPEIALRYGLQGRLA
                     LQFMIGANGQLEQLRLVRSSGSQVLDEEAVRAIRAAAPFPPIPPWIKPNPLLISASME
                     YHDNRLNYRFAR"
     gene            2176..2355
                     /locus_tag="VFY96_18085"
     CDS             2176..2355
                     /locus_tag="VFY96_18085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX5608437.1"
                     /translation="MFGFGLGELLLLLVIVMVVFNRRLPDLGEGLGKSIKKFRRAMNE
                     PDEIDITPKKDSGKS"
     gene            complement(2369..3043)
                     /locus_tag="VFY96_18090"
     CDS             complement(2369..3043)
                     /locus_tag="VFY96_18090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608438.1"
                     /translation="MAEPKDPEVEFVPGREVKNDDPFFHKLEFDDPEQNATNITAISP
                     YLDHRASIKPFLTLFTFLVIGFALLAAIGYARPEAPESVLRKIPVIGAAVLKNNHLKQ
                     GVLIKSLSTSYQTIQGNREVFLVSGIALNQNPVVVREIQLTGTVYNEAGKEIERQTIW
                     AGNTLSPKIIRGMTVEDIPQLQELKPLKSFQLPPGDSIPFTIVFLKSSKNAKEFTCEV
                     STAGEA"
     gene            3560..4114
                     /locus_tag="VFY96_18095"
     CDS             3560..4114
                     /locus_tag="VFY96_18095"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5608439.1"
                     /translation="MELSDWDLVRKCQAGEMASFQELVSRYHQKVYMVVLGLLHNRDD
                     ALEVAQETFFRAYRKIGSFAGGSSFYTWIYRIAVNLAIDTQRRQKRTPLDFRETMDGV
                     LEAQEETARDPFADVHDRELRENLIRAINELTPEHKAVIVLRTIEGLSYKDIGEILGC
                     SEGTVMSRLHYARKKLQDKLSAFL"
     gene            4111..4647
                     /locus_tag="VFY96_18100"
     CDS             4111..4647
                     /locus_tag="VFY96_18100"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX5608440.1"
                     /translation="MRDCETIRESIGRWLDRELDAAESESVRTHLASCEECRKTQQQL
                     ESIQQILEKALLVEAQQIEFLPFWRGVQLRIAQKTSWHERALTWARATFTLSRAAWLV
                     PASIAVILAILSLESYIPGWREGRNSFASVESIDAYGRNVALLRENESKTTVIWLYED
                     QEAENETADESAKSAPAF"
     gene            4604..5071
                     /locus_tag="VFY96_18105"
     CDS             4604..5071
                     /locus_tag="VFY96_18105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608441.1"
                     /translation="MRLLTNPRNQLRLFSLVLILAAGWVGAAEKNPQSVQVTIRTILA
                     ENKSDDYDPKLKGMEQQLKPLKYRSYRLLKAEDQNVLAQGTGSFEIPGGRSLTVAPQE
                     FQNNRIALKVRLTERDKPVIDTTVKIPNKGNFILGGPPHEGGVLVLSISATAQ"
     gene            complement(5086..5340)
                     /locus_tag="VFY96_18110"
     CDS             complement(5086..5340)
                     /locus_tag="VFY96_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018283689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HEX5608442.1"
                     /translation="MASRTKQEIIRERIYLTFPQTLIKEPVLSMLSKQFDIVFNIRGS
                     TVTSDMGLVALEIDGKAAEVAKAIGWLKGKGVTVEPIERT"
     gene            complement(5345..6343)
                     /locus_tag="VFY96_18115"
     CDS             complement(5345..6343)
                     /locus_tag="VFY96_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5608443.1"
                     /translation="MALPSPHLRLVSELSESRKVESVLDLIGNTPLLEINRITEGIPS
                     GVKIYAKLEGFNPGGSVKDRPALRMVQEGLRTQKLYPGKTILDSTSGNTGIALALIGS
                     VLRYPVELVVPGNVSTERKHIIAAYGAKVTYSDPLEGSDGAIRLCREILDADPEKYFK
                     PDQYFNPMNPQAHYENTGPEIYRQTRGTITHFVAGIGTGGTVMGTGGYLKEVNPKIQV
                     IAVEPDDALHGLEGLKHMASSIVPGIYHEEKLDGKIAVSTEDAYSLVYRLSQEEGILV
                     GQSSGAAMFAAIKIARTLRKGTIVTIFPDFGDKYLTTNLWVGWRDRMSVGNLQFSI"
     gene            complement(6358..>6607)
                     /locus_tag="VFY96_18120"
     CDS             complement(6358..>6607)
                     /locus_tag="VFY96_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018285917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoaD family protein"
                     /protein_id="HEX5608444.1"
                     /translation="LRKFTNGADEVDAQGTNMQAIVDDLENHYPGIKERICDESGKVR
                     RFVNVYVNGDDIRFLQNLETVLKEGDNISIVPAIAGGD"
BASE COUNT         1505 a         1695 c         1890 g         1517 t
ORIGIN      
        1 tggggtcgtg cacgatgaaa tacaacccca agatcaacga agttgtcgcc cgcttgccgg
       61 gattcgcaga ggcgcatcca ttagcgccgt ccgagctttt gcaaggggcc ttggccttgc
      121 tgcatgagct tgagctgata cttgcggaga tcagcggcat ggagtacgtc agcttgcaac
      181 ccgctgcggg agctcaaggc gaactgaccg gattaatgtt gattcgagcc tgcctggcgg
      241 agcgtggtga cccgcggaaa aaagttatcg tacccgacac cgcgcatgga accaatccgg
      301 cgagctccgc gttgtgcggt tatgatgtgg tgcagatctc ctccaatgag cgcggtgtca
      361 tcgacgcggt gacagtcgaa aaagcgatgg acgacaatgt tgccgccgtg atgattacga
      421 atccgaatac gctcggtttg ttcgagacta atatcgaggc aatcgccgaa atcgttcacg
      481 cgaagggtgg gatgatttat ctcgacggtg ccaatcttaa tgcgctcatg ggtattgcta
      541 agcccggcca tatgggtgtc gatgtgctgc acctcaatct gcacaagacg ttttccacgc
      601 cgcacggcgg cgggggtccg ggagcgggtc cagtggcggt gacgcaggct ctcagcgaat
      661 ttctgccggt gccgcgtatt atcaagcgcg gccatcgatt tgagctgcag gaggattgtc
      721 ccaagtcggt cggcagggtg cgttcatttt ttggtaattt tggcattctg gtgcgcgcgt
      781 acagttacat tacggccctc ggcgccgatg ggcttgagga tgcaagccgg atggcgctct
      841 tgaatgccaa ctacattcgg aaaaaattgg caaggaatta tcaactcgcc tacaacgagc
      901 catgcatgca cgagtgcatt tttaccgacc gcttgcagca gagaaacggc gtcagcaccc
      961 tcgacatcgc caagcgacta ttagactatg gccatcatcc accgacaatt tattttccac
     1021 tcgtcgtttc cggcgcgctg atgatcgaac cgacggaaac tgagacgccg gaaaccctcg
     1081 atgattttat cgaggcaatg ctcgcgatcg ccgccgaagc caaagaggat ccggagctaa
     1141 ttaaagacgc gccgtattca acgcccgttc gccggctcga tgaggcaaaa gcagcgcgca
     1201 aaccggtgct tcgatgggaa ccctcgtcgg acgccgacaa aaccgaggat tagcagcccg
     1261 gcgtctcctc tgacgaccgc gagcgagtgc ttcagacaac tttcaacggg caaaacgata
     1321 gttgagccgg ttgtcgtggt actccattga agcggagatg agcagcggat tcggcttgat
     1381 ccagggcgga atcggcggga acggcgccgc agctctaatc gcccgcacgg cttcctcatc
     1441 gagcacttgc gatcccgaag accgaaccaa ccgaagctgt tccagttggc cattcgctcc
     1501 gatcatgaat tgcagcgcga ggcgtccctg gagcccgtag cggagagcga tctccggata
     1561 ttgccactgg gtttcgatca attgtttgat tttggtgaag taggtaacat acacgggatc
     1621 gttggtattg aggcttatgg gcatatcggc gcgggcgctt gaggatgacc aggtggcgct
     1681 gggcaacagc tctttcaggg ttggcagctg acgctctgcg atcgcggtct cctcaagggg
     1741 ttgctcacgc ggtgctgctg gccgcgggtt ctcaggaggc ttgtcaacgg ttaccggagg
     1801 cagctccgga gcagccggca ctctggcgcg ggaagaggtt gggttgttgg gggagagacg
     1861 agaatctttt ttggcgacaa ctgagggggc tctcgacatg cgggtcggcg gcgttcgcgg
     1921 cgtccggatc ggcgcgggct ccgctgtttc tgacggcggc aaaagcgaga cggaaattgt
     1981 ctccggctga gctcgaattg ctccagctcg caacggatag atcatgagca ctatcacggc
     2041 atgggtcgcg agcgacagag cgaaaaaccg ccatactgtg gtggagtccg ccatcatcgt
     2101 atattatcga gtcacagtgc gcgaggaaag ccgcgcaaac tttcgcgcgc agcgcgttcc
     2161 cgggaggtga tcttcatgtt cggttttgga ctcggcgaat tattactgct tctggtgatt
     2221 gtgatggtcg tgttcaatcg acgtctgccg gacttgggcg aggggctcgg caaaagcatc
     2281 aagaaatttc gccgagccat gaatgagccg gacgaaatcg atattacgcc caagaaggac
     2341 tcaggcaagt cttagatctg cgccgcactt aagcctcacc cgcagtgctt acttcgcacg
     2401 tgaattcctt tgcgtttttc gatgacttga gaaaaacaat tgtaaacggt atcgagtctc
     2461 ccggcggcag ctgaaaactt tttaaaggtt tgagctcctg caattgtggg atatcctcca
     2521 cggtcatgcc gcggatgatc ttcggcgaaa gggtgttgcc agcccaaatc gtctgccgtt
     2581 caatttcctt gcccgcttca ttgtaaacgg tacccgtgag ctgaatctcg cgcacgacga
     2641 caggattctg gttcaaggcg ataccgctca ccaaaaaaac ctcgcggttg ccctgaatgg
     2701 tttggtaact cgtgctcagt gatttgatca gaacgccttg tttgagatga ttattcttaa
     2761 gcacggccgc gccgatcacg ggaatctttc gtaaaaccga ctccggggct tcaggccgtg
     2821 cgtagccgat ggctgcgagc agcgcaaacc cgatcacgag aaaagtgaag agcgttaaga
     2881 aaggctttat cgacgcgcga tgatcgaggt aaggagagat cgccgtgatg ttcgttgcgt
     2941 tctgttcagg atcatcgaac tccaatttgt gaaaaaacgg atcatcgttt tttacttccc
     3001 tccccggcac gaattcgacc tcgggatcct tgggctcagc caagacaaac ggtttctcct
     3061 gcggttgtgc cgtgtggcct atgccccact ggtggtgcgc tgcgcttgaa ctcgcaataa
     3121 cgtttgttcg cctttccggt gaaagatgtt cctgttcgga ttcgctagag tctgcgctgc
     3181 ttctcggttt tacaccaaaa ggcaggctca gctcatcggc ggccgacggc ggcacgattg
     3241 tcgcgtcagc gggttgaaag gtttcagagc ttggctcacg atattgatcg ctttcgaact
     3301 cgaaggtgtg tttgcaacgt gagcagcgaa acgccgcagc ggctcccctc aacacgtcat
     3361 cggacacctt gtaagtggtt tgacattttg ggcattgaac tagcatcgga gtcccttttg
     3421 gtcgatgaac ggcaattggg aaaaggcaag taaaaacaaa gtgattctag attaattcgc
     3481 gaaaacgtca aggttttatg ttattaactc agcgcggtga ataaaaattt tctaagttcg
     3541 tctaaaaggt acgatcgttg tggagttgag tgactgggat cttgttcgaa aatgccaagc
     3601 gggagaaatg gcttcgtttc aggagcttgt ttcccgttac catcagaagg tttatatggt
     3661 ggtcctgggg ctcctacaca atcgggacga cgcgttggag gtcgcgcaag aaaccttttt
     3721 tcgagcctat cgcaagatag ggagttttgc agggggttca tcgttctaca cctggattta
     3781 tcgaatcgca gtcaatttgg cgatcgatac gcagcggcgg caaaaaagga ccccactcga
     3841 ttttcgtgaa acgatggatg gcgtattgga agctcaagag gaaaccgcga gagatccctt
     3901 tgccgatgtt catgatcgag agctacggga aaatctgatt cgagcgatca acgaattgac
     3961 gcccgaacac aaggcggtca tcgtgctcag gaccatcgag ggtctttcat acaaggacat
     4021 cggagaaatt ctgggctgtt ccgaaggcac ggtgatgagt cgcttgcatt atgcgagaaa
     4081 aaagctgcag gacaagttga gcgcgtttct atgagagatt gcgagactat acgagagtcg
     4141 atcggacgct ggctcgaccg cgagctcgat gctgcggaga gtgaatcggt ccgcacccat
     4201 ctggcaagct gcgaagaatg tagaaaaacg caacagcaac tcgaatccat tcagcaaatc
     4261 ctggaaaaag ccctgctggt cgaagcgcag caaatcgagt ttttgccgtt ctggcgcggc
     4321 gtgcagcttc gcatcgcgca aaaaacttcg tggcatgagc gggcgctgac gtgggcacgc
     4381 gcgacgttta cgttatctcg agcggcatgg ctggtgcccg cgagtatcgc cgtaatacta
     4441 gccatcctat cgctggaatc ctacattccc ggctggcggg aggggcggaa cagttttgcg
     4501 agcgtcgaat ctatcgatgc ttacggccgc aatgtcgctt tgctgcggga aaatgagagc
     4561 aaaaccactg tgatttggtt gtatgaggat caagaggcgg aaaatgagac tgctgacgaa
     4621 tccgcgaaat cagctccggc tttttagtct cgttctgatt ttagcagcgg gatgggtcgg
     4681 cgcggcggaa aagaaccctc agtccgtaca ggtgacgatt agaaccatac tcgccgaaaa
     4741 caaaagcgac gactacgacc ccaagctgaa gggaatggag cagcaactca aaccgctcaa
     4801 gtaccgctcc taccgtttgc tgaaggctga ggaccagaac gtgctcgcac aaggaacagg
     4861 atcctttgaa attccggggg gccggtcttt aacggtggcc ccgcaggaat ttcaaaacaa
     4921 ccgtatcgct ctcaaagttc ggttgaccga gcgcgacaag ccggtcatcg ataccacggt
     4981 gaaaattccc aataaaggga actttatttt gggcggcccg ccgcatgagg gcggcgtgct
     5041 ggtgctgtcg atttccgcta cggcgcagta agctcgtcta ttcgatcacg ttctttcgat
     5101 tggctcgacc gtaacacctt ttcccttcag ccagccaatc gccttggcca cttcggctgc
     5161 tttgccgtcg atttcgagag cgacgagacc catgtctgaa gtgacagtgg agccgcgaat
     5221 attgaagacg atgtcaaatt gcttcgataa catgctcaac accggttctt tgatgagagt
     5281 ctgaggaaac gtcagataga ttcgttcccg aatgatttct tgtttggttc ggctcgccat
     5341 ggcttcagat cgaaaattgc agattgccga ccgacatccg gtcgcgccag cccacccata
     5401 aattcgtggt caggtattta tcgccaaagt ccggaaaaat ggtcacgatg gtccccttgc
     5461 ggagagtgcg cgctattttg atggcggcaa acatcgcggc tccggaagat tgtccgacaa
     5521 gaatcccttc ttcctggctt agccgataca cgagggaata agcgtcctcg gtggagacgg
     5581 caattttgcc gtccaatttt tcttcgtgat aaattcccgg cacaatggag ctcgccatgt
     5641 gcttcaaccc ctcgagcccg tggagagcgt catccggttc aaccgcaatc acctgaatct
     5701 tcggattgac ttccttcaag taaccgccgg tgcccatcac ggttccgcca gtgccgatcc
     5761 cggcaacgaa atgggtgatg gtgccgcgcg tctggcgata aatttctggg ccggtgtttt
     5821 cataatgggc ctgcggattc atcgggttga aatactgatc cggcttgaaa tatttctctg
     5881 gatcggcgtc gagaatttcg cggcacaaac gaatcgcacc atcggagcct tccaagggat
     5941 cgctgtaagt aaccttggca ccatacgcag cgatgatgtg tttacgttca gtgctgacgt
     6001 tgccgggaac gaccagttcg accggataac gcaaaacgct accaattaac gccagtgcaa
     6061 tgccggtgtt gccggaggta gagtcgagga tggttttacc aggataaagt ttttgcgtgc
     6121 gcagaccttc ctgaaccatg cgcaacgctg gccggtcctt gacggagccg ccgggattga
     6181 acccctcgag ttttgcgtaa atttttacgc cggatggaat tccctcggtg attcgattga
     6241 tctcgagtaa aggcgtattg ccgatgagat cgagaaccga ttcgacctta cgcgactcgc
     6301 taagctctga aaccagacgc aaatgcggcg agggtagcgc cataaccggc cgtggcttca
     6361 gtcgccgccc gcaatggccg ggacgatcga gatattgtcg ccctctttga ggacggtctc
     6421 gagattctgc aagaaacgaa tatcgtcgcc attgacatag acgttgacaa aacgacgcac
     6481 tttcccgctc tcgtcgcaaa tccgctcttt aataccggga taatggtttt ctagatcatc
     6541 gacgatcgct tgcatgttag tgccctgcgc gtcgacctca tcggcgccgt tggtaaattt
     6601 tctgagc
//
LOCUS       DASULZ010000877         2988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_404948, whole genome shotgun sequence.
ACCESSION   DASULZ010000877 DASULZ010000000
VERSION     DASULZ010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404948"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(535..1518)
                     /locus_tag="VFY96_18125"
     CDS             complement(535..1518)
                     /locus_tag="VFY96_18125"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5608445.1"
                     /translation="MKLKEFFYPALILLILARAVGLVAAEPFRVGFPSLATGFAPSWV
                     AADKGIWKKHGLDVELIFLRGGSRTVSALLGGSVDFIIGSDLGVTTAILQGAALTRVG
                     TTTNTLGYSMVTQPGIKTVRDLKGKVIGITPGRDAAYARVVKLLRDNGMDASKDVTFL
                     SVGDGGPAARVAALSSGVIHATMFTPPSDMISEKAGMRILTKIDVANIGGGLNTSTAA
                     VQKNRAQLLRFLRGYMEAIQYLKSHKDESLKIFSKYVRNPDLNIMAYLYDEISMRAEP
                     SLRPQADAVRALLDLAALDFPQAKRLSEKDNWDLSLLDEIQKSGFFEQLYR"
     gene            1647..2894
                     /locus_tag="VFY96_18130"
     CDS             1647..2894
                     /locus_tag="VFY96_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HEX5608446.1"
                     /translation="MPRSVVHISGHIIDSLILPKILDIIISLGAEFQILDIHIGQRRA
                     DRSSARVQIDAPTEESLQQILAKVKEHGALPLDTEDEVVRFEAAPTDGIFPGDFYATT
                     NLPTLIRLDDQWVEVEHPEMDCGIRVLPQSRRAETVPMHRVKKGDFIVIGHGGVKIVP
                     MERHVPREPFEFMSSSVSTEQPKGLMIREIADSMIRARAAQGRILVVAGPGLVHTGAA
                     RHLIELIENDFVQVLFAGNGLAVHDIESALYGTSLGVYLDKNIRASEGHEHHLHAINA
                     IRKVGGIRQAVKQGLVQRGIFYSCVKKGVDFVLAGSIRDDGPLPDVITDTLQAQEAMR
                     EKVRTVSFALMMATTLHAIATGNLLPATVKTVCVDINPAVVTKLCDRGTFQAVGLVTD
                     VEPFLRELSQYLRQGDNPADAHQ"
BASE COUNT          675 a          826 c          842 g          645 t
ORIGIN      
        1 attgcggaac cagcgccagc aaacatgcta attgcactcg gcatgttcaa cattcttgaa
       61 ggagggtgaa tatgtcagtt ttcaaaaaaa tttgcagcgc gttcttgctg ttttcgctcc
      121 tggcgcttgg cggatgcccg gagacggctc agcagggcgg atcgtcatca ggccagtcca
      181 gtggtggaag cggcggaggc gcaggagact aatagcctaa cctcgcacag cgcttcggcg
      241 aaagcgcagc gaagccggag tgctgtgcct ggtttttcgc agtaagcttg tcagcgaatt
      301 tgtgtttgca ccgtcctgcg gcgtagctgt gggtggtcgc cgtcggcggc gcgttacctc
      361 ttgtaaatcc cgtcaataaa tccgctctgc tcgaacggtc tcaggaaaga tggatctata
      421 attagattag catcggtttg tttggcttga ggatattcga gggataccag tccgcttttg
      481 atgaccaggc gaaggcaaga gcagctgctc cctcggcatc gatcgtcaac cgaactagcg
      541 gtacaactgc tcgaagaaac ctgatttctg gatctcatca agcaggctca gatcccagtt
      601 gtctttctcg cttaaacgct tggcttgggg aaaatccaac gccgccaaat ccagtagagc
      661 gcgcaccgcg tccgcctgcg ggcgcaagct cggttcggcg cgcatcgaga tctcgtcgta
      721 taaataggcc atgatgttaa gatccggatt acggacatac ttggaaaaaa tcttcaggct
      781 ttcatccttg tgcgatttca aatactgaat cgcctccata tagccacgca agaaacgtaa
      841 gagttgagcc cgattcttct gcaccgctgc ggtcgaggtg ttgagtccgc caccgatgtt
      901 ggccacatcg attttcgtga gaatgcgcat acccgccttc tccgaaatca tatcggaggg
      961 cggcgtgaac atggttgcat gaataacgcc gctcgagagc gccgccactc gagcggccgg
     1021 tccgccatcc ccaaccgaca ggaacgtcac gtctttgctc gcatccatgc cgttgtcgcg
     1081 cagtagctta accacccgcg cataggcggc gtcgcggccg ggtgtgattc cgatgacttt
     1141 gcccttcaga tcgcgcaccg ttttgatacc gggttgcgtg accatcgaat aacccagagt
     1201 attggtggtc gtcccgactc gcgtcagcgc cgctccctgc agaatcgccg tggtgacacc
     1261 gagatccgag ccgatgatga aatcaacgct gccgccgagc aaagctgata cggtccgcga
     1321 cccaccacgc agaaaaatca gttcgacatc caagccgtgc ttcttccata ttcctttatc
     1381 cgccgccacc caggaaggag caaaaccagt cgcgagtgat gggaagccga cgcgaaacgg
     1441 ttcagcggcg acgagtccta ccgcgcgcgc gagtatcaat agaataaggg cgggatagaa
     1501 aaattcctta agtttcattc cgtgacctga atcaaggcga cgggcagagt gaatataaca
     1561 caaccgcgtc caccaaatct gcatgcgagt cttgtgttgt cgttgcctcg gcaggctagt
     1621 aaaatatcac ctctcgggag gttcgtatgc cgcggagcgt cgtgcatatt tccggtcata
     1681 ttatcgactc cctgattctc cctaaaatcc tcgacatcat catcagcctc ggcgccgagt
     1741 tccaaatcct cgatattcat atcggacagc gacgcgccga tcgcagctcg gcgcgggttc
     1801 agatcgacgc acctacggaa gaatcactcc aacaaatatt ggccaaggta aaagagcacg
     1861 gcgcgttgcc gttggacacc gaagatgagg tcgttcgatt cgaagccgcg cctacggacg
     1921 gaatttttcc cggtgatttt tacgcgacga caaatctgcc gaccttgata cgcttagacg
     1981 atcagtgggt cgaggtggaa cacccggaga tggactgcgg gattcgagtg ctcccgcaaa
     2041 gccgacgtgc ggaaactgtt cccatgcacc gggtcaaaaa aggcgacttt atcgtgatcg
     2101 gccatggagg cgtgaagatc gtacctatgg agcggcacgt gccgcgggaa ccatttgagt
     2161 tcatgtcgag ctcggtttcg acggaacagc caaagggatt gatgatccga gaaatcgccg
     2221 actctatgat tcgggcgcga gcggcgcaag gtcgcattct ggtcgtggcc ggtcccgggt
     2281 tggtgcacac gggcgcggcg cgccatctca tcgagctgat tgagaatgat tttgtccagg
     2341 ttctgttcgc cggcaatggg ttggccgtgc atgatatcga aagcgcgctc tacggcacat
     2401 ctttgggcgt ctatttggat aaaaacattc gggccagcga aggccatgag catcacctgc
     2461 acgcgatcaa cgcgataaga aaagtgggcg gaatccgcca agcggtcaaa caaggcttgg
     2521 ttcagcgtgg catattttat agctgcgtca aaaagggcgt ggattttgtg ctggcgggct
     2581 ccatccgcga cgatggaccg ctgcccgacg tcatcaccga cacgctccaa gcgcaggagg
     2641 cgatgcgcga aaaggtgcgc acagtgagct ttgcgttaat gatggcgaca acgctacatg
     2701 cgatcgccac cggtaatctc cttccggcaa ccgtcaagac agtgtgcgtc gacatcaatc
     2761 ccgctgtcgt caccaagctt tgcgatcgcg gcacttttca ggcggttggc cttgtgaccg
     2821 acgtcgagcc tttcttgcgc gagctttccc agtacctcag gcagggcgac aaccccgcgg
     2881 acgcacatca atgatgctct gcagtactga gcggaggata gaatgcgcct gttgatgtgc
     2941 ccgccggctc actacggcat cgaatatgag atcaatccct ggatgagc
//
LOCUS       DASULZ010000878         4909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_304320, whole genome shotgun sequence.
ACCESSION   DASULZ010000878 DASULZ010000000
VERSION     DASULZ010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4909
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304320"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VFY96_18135"
     CDS             <1..192
                     /locus_tag="VFY96_18135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5608447.1"
                     /translation="DMQGTFFRIVRTLRTYPLAAAGVSSFVISGFGKNLFRSARLVFK
                     LRQLVLPLWLWWVRRRHDS"
     gene            complement(202..1875)
                     /gene="ggt"
                     /locus_tag="VFY96_18140"
     CDS             complement(202..1875)
                     /gene="ggt"
                     /locus_tag="VFY96_18140"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5608448.1"
                     /translation="MRFRIFFSSVLLCTFLAAGAGARTLQSSAGLVVADSELASEAGM
                     EILRRGGNAVDAAIATAFALSVVDQASSGLGGGGFMVIYRAKDQQAFTLDFRETAPQA
                     ARRELYTKDGKPVAALSLTGGLAVAVPAEVAGLVEARKRFGTLSLATLVAPAIKLANE
                     GFALDPTLRVAIERQQSAMKRFTELGRVFLPNGMVPREGDVIRQPDLGATLKTIAEDG
                     GENFYHGRIAAAIVNTVQSSGGVISRDDLKHYKPVWREPLTGSYRDRRVITMPPPSSG
                     GVAILQMLNILEGYNLGQMEHDSSPYLHLVTEAMKHAFADRAQFLGDPDFVHVPVTKL
                     TAKSYAEWVRHRISPEKTQPTSFYGYYHYNAEKGGTTHFSVVDRFGNAVSCTQTVNTR
                     FGSKLLAAGTGIVLNNEMDDFAIHGAVGNVYGLIGNEANALQPKKRPLSSMAPTIVLR
                     QGHPELVVGAAGGPRIISATLQTILNVLDFHMPISSAVEAPRIHHQWIPDRLNFEAGI
                     SPQTRKGLEERGHTLREQSSLGVAQGIARQGAQLSGAADSRKIDRARTE"
     gene            2051..>4909
                     /locus_tag="VFY96_18145"
     CDS             2051..>4909
                     /locus_tag="VFY96_18145"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX5608449.1"
                     /translation="MQTILGPFHPDLETAFVDEILTCKATDLLSPLLVLTPSDSLRRR
                     LKFLLARERGLSLLNLQLLTFHQLALRLHEESNGPPFDLQRDLFFEETLRQLIRSRCP
                     GAEPFLGLEERAGGCAALWQTLRDLRDGLVDPTVALDALAEGHFGPTAERTSALLHLL
                     QSFQQFCGAQNITSESDLNRSVTEQVPSSKYLKQFSRVFYYGFYDLTQIQLDFFHAVA
                     RHFPTTLFFPLLAANPSHDAWRFAERFYERYIEGHTSSSAQKLSSASFLPAHARLFDT
                     MRNRSFAAFPNDWRCQITSAFGIHDEVATVAKQILAQLDLGKIPFHEIGVVARSLESY
                     APIIKTIFHQHCIPVSGRLEEPLAKEPLTKAVILLLNLPAKDFPRSQVIDLLSSPYFQ
                     LQNIHPDPASVRPDLWDLATRELAICKGIGEWRRLRRYRHRDLELRQVSDDEEPRRIR
                     IRSTELTSLADTVETLIADLLRLPAEASWRQYAAAWKLFLNKYLGLAEYTETRPVTVS
                     KPAETILGILNELGGLDRAQDRVILGEFVHTFQHWLERSSIIDDRRNIDGVTVFNATA
                     ARGLSFRVLFVLGTNEGVFPRAIREDAFLRDREREVLERDLGYKVSQKLTAFDEEKLL
                     FTLLVGAAREQLYCSFQRADENGRALAPSWYLDELKRALEESHRPCETVDIPRSVTEK
                     AATAPYQREDLLLPSEWAIRLSLQGRDPTPLIEAFAPLPAIYRHGKKVVGEIDRSGDR
                     LLAYDGALTDFQNYWERYSERGVSPTALETYARCPFQFFARHVLGLAPLDRPEEVLGP
                     SPAEFGELGHGILHSFYTALIEARYFADPRASVDVDHELEIAANQVFAEYQENHPIGY
                     PLAWEILKTDILALLRKVVAQDLSELKTSGFAPMSLENNLLTHLPDDWPEPLNHLLIR
                     GRLDRIDCKDDAVRVIDYKFKLGTSAATPDKNLVRAALR"
BASE COUNT          983 a         1490 c         1315 g         1121 t
ORIGIN      
        1 gatatgcaag gaacgttttt tcgaatcgtt cggacgctca ggacttatcc gttggcagcg
       61 gcaggggtat cgagttttgt catcagcggc tttggcaaaa atctgtttcg ctccgcccgt
      121 ctagttttca aactccgcca actagtgttg ccgctctggc tttggtgggt aaggcgccgc
      181 cacgactctt gatgctgcga gctattccgt ccgtgccctg tcgatcttcc tcgaatcggc
      241 cgcaccactg agttgagcgc cttgtcgcgc gatcccttgc gctacaccca aggagctctg
      301 ttctctcaac gtgtggccgc gctcctcgag cccttttctg gtttgaggac tgattccggc
      361 ttcgaagttc agccgatcgg gaatccattg atgatggatc cgtggcgctt cgactgccga
      421 gctaatcggc atgtgaaaat ccagcacgtt gagaattgtc tgcagagtcg cgctgatgat
      481 ccgtggtccg cctgcagctc ccaccaccag ctccggatgc ccctgacgga gcacgatggt
      541 tggcgccatg ctgctgagcg gccgtttctt cggctgaagc gcattagcct cattgccgat
      601 gagcccgtag acgttcccga ctgccccatg aatggcaaaa tcatccattt cattgttgag
      661 cacgattccc gttcccgccg cgagcagttt ggaaccgaaa cgtgtgttga ccgtttgtgt
      721 gcatgacacg gcgttgccga accgatccac aacgctaaag tgagtcgtgc cgcctttttc
      781 ggcattgtag tgatagtagc cgtagaaact cgtcggctgc gtcttttccg gggaaattct
      841 gtggcgcacc cactcggcgt aacttttggc ggtcagtttg gtcactggaa cgtggacgaa
      901 atcgggatcg ccgagaaact gcgcgcggtc ggcaaatgca tgtttcatcg cctcggtcac
      961 cagatgcaaa tacggcgagg agtcgtgctc catctgaccc agattgtatc cttcgagaat
     1021 gttgagcatc tgcaagattg ccactccgcc tgaactgggc ggcggcatcg tgatgacgcg
     1081 ccggtctcgg tagcttccgg tgagcggttc gcgccaaacg ggtttatagt gcttgaggtc
     1141 atcgcggctg atcacgccgc cactggactg caccgtattg acaatcgcgg cagcgatacg
     1201 tccgtgataa aagttctcac cgccgtcctc ggcaatcgtt ttgagagtcg cgcccaaatc
     1261 cggttggcga atgacgtcgc cctctctggg aaccatcccg ttcggcagaa aaacccgccc
     1321 gagctccgta aagcgcttca tcgcagactg ttgtcgctcg atcgcgactc gcagcgtcgg
     1381 gtccaacgcg aaaccttcat tcgcaagttt gatggcgggc gcaaccaggg tagccaatga
     1441 aagcgtgccg aagcgcttgc gcgcctcgac caatcccgcc acttcggccg gcacagcgac
     1501 ggccagtcca cctgtcaggc tcagcgccgc cacgggtttg ccgtccttcg tatagagctc
     1561 gcgccgtgcc gcctgcggag cggtctcgcg aaaatccagc gtaaacgcct gttggtcttt
     1621 ggcccgatag atcaccatga atccgccgcc gcccagaccc gacgacgctt gatcgaccac
     1681 cgaaagcgcg aaagcagtgg caatcgccgc atccacggcg ttccccccgc gccgcaagat
     1741 ttccattccg gcttcactcg ccaattcgct atcggcaacg acgagcccgg cgctcgactg
     1801 cagagtccgc gcgccggctc cggcggcgag aaaagtgcag agtaaaaccg acgagaagaa
     1861 gattcgaaat cgcatagcag actcaataag acgactttga tttaaagctt tgtctgctga
     1921 tttgcaactt agcacgcacg ccattgatgg ctgaacgacg caccgccacg attttcactt
     1981 gcgcttgtcg ggagcttatc tcatattgat tatcgattat gctctccgtg caccgactga
     2041 tatcctaacc atgcaaacca ttctgggacc gtttcatccg gatctcgaaa ccgcctttgt
     2101 cgatgaaatc ctgacatgca aagcgacgga tttgctttca ccgctcttgg ttcttacgcc
     2161 ctccgattcg cttcggcgtc ggctaaaatt tctcctggct cgtgagcgtg gactctcgct
     2221 gctcaatctc cagctcctga ctttccatca attggcctta cggttgcacg aagagagcaa
     2281 tggaccgccg tttgacctcc aacgggatct gtttttcgaa gaaacactcc gacaactgat
     2341 tcggagccgt tgcccgggcg cagaaccgtt tctcggtctc gaagaacgcg ccggcggctg
     2401 cgccgcgcta tggcagacct tgcgcgatct ccgcgacggt ttggttgacc ccacggtcgc
     2461 cctggatgcc ctcgctgaag ggcattttgg tcctacggcg gaacggacat cggcgctgct
     2521 gcatctcttg cagagctttc aacaattttg cggcgcgcag aacatcacca gtgaatcgga
     2581 cctgaaccgc tctgttaccg agcaagttcc ctcttcgaaa tatctgaagc aatttagccg
     2641 cgtgttttat tacggatttt atgacttgac ccaaatccag ctggattttt ttcacgccgt
     2701 ggcgcgccac tttccgacca ccctgttttt tcctttgctc gccgccaacc cgagtcatga
     2761 cgcctggcgt ttcgcggagc ggttctatga gcgctatatc gaaggtcata ccagcagctc
     2821 ggctcaaaag ctgagttccg caagcttttt gccagcgcat gcccggctct tcgacactat
     2881 gcggaacagg agtttcgcgg cgtttccaaa tgactggcgc tgccaaatca cgagcgcttt
     2941 cggtatccac gacgaggtag cgacggtggc gaaacagatc ctcgctcagt tggatcttgg
     3001 caagatacct tttcacgaga ttggcgtcgt tgctcgatct cttgagtcct atgcgccgat
     3061 tatcaagact attttccacc agcattgcat tcctgtttcc ggccggctgg aggaaccctt
     3121 agcgaaggag cctttgacca aagcggtcat cctgctgctc aatcttccgg ccaaagactt
     3181 cccgcgcagc caggtcattg acctgctctc atcgccttat tttcaactcc aaaacatcca
     3241 tcccgatcca gcttcggttc gacccgacct ttgggatctt gccacccgtg agcttgccat
     3301 ctgcaaaggg atcggcgagt ggcgccggct ccgccgttat cgccatcggg atttggagtt
     3361 gcggcaggtc tccgatgatg aagagccgcg caggattcgt atccgctcca cggagctcac
     3421 atctctcgcc gatacggttg agaccttgat cgcggatctg ctgcgcttgc ccgccgaagc
     3481 ttcatggcga caatacgccg ccgcctggaa gttatttctc aataaatacc tcggcctcgc
     3541 cgaatacaca gagacgcgcc ccgtgacggt gtcgaaaccc gctgaaacca ttctcggaat
     3601 tttgaacgag ctcggcggtc tggaccgggc gcaagatcgg gtcatcctcg gcgagtttgt
     3661 tcatacgttt cagcattggc tcgagcgctc gagcatcatc gacgaccggc gcaacatcga
     3721 cggcgtcacg gtgttcaatg ccactgcggc gcgcggctta tcattccgcg tactttttgt
     3781 gctcggcacg aacgaaggag ttttcccgcg cgcgatccgg gaagacgctt ttctgcgcga
     3841 tcgtgagcgg gaagtgctcg aacgcgacct aggatacaag gtcagccaaa aactcaccgc
     3901 tttcgatgag gaaaaattgc tcttcacgct gctggtcggc gccgcgcgcg aacaactcta
     3961 ttgctccttt caacgcgcgg acgagaacgg cagggcattg gcgccgtctt ggtacctcga
     4021 tgaactcaag cgcgctctcg aggagagtca ccggccctgc gaaactgtcg acattccccg
     4081 cagtgtgacc gagaaagctg caaccgcacc ataccagcga gaagatctgc tgctcccatc
     4141 cgagtgggca attcgcctga gccttcaagg tcgcgatccg actccgctga ttgaagcctt
     4201 cgcaccgctg ccggcgattt accgccatgg aaaaaaggtc gtcggcgaaa tcgatcgcag
     4261 cggcgatcgt ttgctggcct acgacggggc gctcactgac tttcaaaatt actgggagcg
     4321 ttattccgaa cgcggggtct cgccgactgc gctggaaacc tatgcccgct gtccgtttca
     4381 gttttttgcg cgccacgtac tcggcttggc acctctcgac cggcccgaag aagtcctcgg
     4441 accgagtccc gctgagttcg gtgagctcgg ccatggaatt ctccattcgt tttacactgc
     4501 cctgatcgaa gcgcgctatt ttgccgaccc tcgggcatcc gtggacgtcg atcacgaatt
     4561 ggaaatcgcc gcaaaccagg tcttcgccga gtatcaggaa aatcatccga tcggttatcc
     4621 gctcgcctgg gaaatcctga aaacagatat tctcgcactg ctgcgcaaag tcgtcgccca
     4681 ggatcttagc gagttgaaga cgtccggatt tgctcccatg agcttggaaa ataatttgct
     4741 cacgcacctg ccggacgact ggccggaacc gttaaatcat ctcctgatcc gcggtcgctt
     4801 ggatcgcatc gactgcaaag atgacgctgt gcgggtgatc gattacaagt tcaagctcgg
     4861 cacaagcgcg gcaaccccag acaaaaatct tgtccgcgcc gcgctccgc
//
LOCUS       DASULZ010000879         2712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Binatia bacterium isolate SMAG_U1558
            k141_405832, whole genome shotgun sequence.
ACCESSION   DASULZ010000879 DASULZ010000000
VERSION     DASULZ010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961700
            Sequence Read Archive: SRR25158530
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Binatia bacterium (soil metagenome)
  ORGANISM  Candidatus Binatia bacterium
            Bacteria; Candidatus Binatota; Candidatus Binatia.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 09:42:12
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,633
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,593
            CDSs (with protein)               :: 3,593
            Genes (RNA)                       :: 18
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 6 of 22
            Pseudo Genes (incomplete)         :: 16 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 2 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Candidatus Binatia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405832"
                     /isolate="SMAG_U1558"
                     /isolation_source="soil"
                     /db_xref="taxon:2838779"
                     /environmental_sample
                     /country="China"
                     /lat_lon="37.52472222 N 121.3841667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..535)
                     /locus_tag="VFY96_18150"
     CDS             complement(137..535)
                     /locus_tag="VFY96_18150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007981659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX5608450.1"
                     /translation="MAATIPENFKDLFSKVAFAQLATLMPDGSPQVTPVWVDYDGTHV
                     RVNSAKGRLKDKNMRRDKRVALSIQDPENAYRYIAVRGDVVEITEQGADAHIDALAKK
                     YLGKDRYPFRSVGEVRVIYKIRPDKVSTYG"
     gene            complement(559..1206)
                     /locus_tag="VFY96_18155"
     CDS             complement(559..1206)
                     /locus_tag="VFY96_18155"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX5608451.1"
                     /translation="MKRSVDLVMFDLDGTLADTGRDLAESVNYTRSQFDLASLAAHSV
                     YRHIGRGVDHLLKHAIPELGRDHFEEVRHLFLRHYEQHLLDQTALYPGILKVLDDFSS
                     KRRAVVSNKFYSLAVKLLKGLGVENCFDAIFGGDSAADKKPDPALLHAALTQFRLPAA
                     RAVMVGDGDIDIEAGKRAGVMTCGVTYGLGDKNDLMAARPDVIINQPIELGNYFV"
     gene            1325..2317
                     /locus_tag="VFY96_18160"
     CDS             1325..2317
                     /locus_tag="VFY96_18160"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5608452.1"
                     /translation="MELKSPVFALILLVLSICADSAWAADKLNTAYISTTPGSSSVLW
                     VAKDAKIFDKYGIDATIIFISGSIRGIQSILAGEIPIGEGGGPGLASARLAGGDVVAI
                     AGNVNVLPYYLVAQTNIKKPEDLRGKIGGNHIAGTTAEFALKVGLKKIGIDPFKDVNL
                     RVIGGSIERMVALQKRIVDFTVVTEAGKAMAEKLGYPTVVDMVALKIPFPQNGIFTST
                     KLIRDNPDFVRRYMRAYVEAIHFYKTHKEETIKTMRKYSRVDDRKFLDEAWDWHVKFM
                     PDAPYPPAEGYQLVLQDMAEKNPKAAQAEIKDYIDARFVKELEDSGFIKGLSRK"
     gene            complement(2436..>2712)
                     /locus_tag="VFY96_18165"
     CDS             complement(2436..>2712)
                     /locus_tag="VFY96_18165"
                     /inference="COORDINATES: protein motif:HMM:NF016601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HEX5608453.1"
                     /translation="DQYGPENLTVILGNGEANGVEVFAETVTEGDPSYAGPLAGVALK
                     IPVYHILEDEVMSQVPESLREEKLQLSALVVDVGPMHQVLEASRQKI"
BASE COUNT          631 a          753 c          693 g          635 t
ORIGIN      
        1 aaggtcgcgg ctttggcggc actcaataag ttctccaaca gcagtaccgt ttgcgccatt
       61 gaatagctgc tgtcgtgagt gttccgcggt tgccgcgctt cctagccggt cactcagagt
      121 tcatacgaag tatgactcag ccatatgtcg aaaccttgtc aggccggatt ttatagatca
      181 ctcgcacctc tccgacgctg cgaaaaggat agcgatcttt gcctaaatac tttttggcca
      241 gggcatcgat gtgcgcgtcg gcaccctgtt cggtgatctc aacgacgtcg cccctgactg
      301 caatatagcg ataggcattt tccggatcct gaatcgacag cgcgacccgc ttgtcgcggc
      361 gcatgttctt gtccttcagg cgtcctttcg cggaattcac tcgaacatga gtaccatcat
      421 agtcgaccca aaccggggtt acctgcggac tgccatccgg catcagcgtc gctaactggg
      481 caaatgccac tttgctaaaa agatccttga aattctcggg aattgtcgca gccatgattc
      541 acctcctgtt atttcgattt aaacgaaata attgcccaat tcgatcggct ggttgatgat
      601 cacatcgggg cgtgcggcca ttaagtcgtt cttatcaccg agtccatagg taaccccgca
      661 ggtcataacg cccgcgcgtt ttccggcttc aatgtcaatg tcaccgtcgc ccaccatcac
      721 cgcgcgcgcg gcgggcaagc gaaactgtgt caacgcggca tgcaaaagcg ctggatctgg
      781 tttcttgtcg gctgcgctgt cgccgccgaa gattgcgtca aaacaatttt ccacgcccaa
      841 gcctttcagc agcttgacag cgaggctata gaatttattg ctcaccactg cacgccgttt
      901 gctgctgaag tcatcgagca ctttaagaat ccccggatag agcgccgttt ggtcgagcaa
      961 gtgttgctcg taatgtctga ggaaaaggtg ccggacctct tcgaaatgat ctcgccccag
     1021 ttccggtatt gcatgcttga gcaagtgatc gactccgcgc ccgatatggc gatagacaga
     1081 atgtgccgcc aatgaggcaa gatcgaattg gctgcgggta taatttaccg actcggcgag
     1141 gtctcgtccc gtatcggcta atgttccgtc caggtcaaac atcaccaaat cgactgatct
     1201 cttcaccgcg ttcgattcaa gttgaaaaca gtctcgaagt caacgcgctg ccgaaagccg
     1261 ctttgctctc ccgccgtcaa atggtgtagt ttggctctca gctagaacgc gtctggagga
     1321 acctatggaa ctcaaatcgc cggttttcgc tcttatactc ctggtactga gcatctgcgc
     1381 agactcggcc tgggccgcag acaaactaaa cacggcttac atctcgacta cgccaggttc
     1441 ttcatccgtg ctgtgggtcg ccaaggacgc gaagattttt gacaagtacg gcattgacgc
     1501 gacgattatc tttatttccg gcagcatccg cggcattcaa tcaatccttg ccggcgagat
     1561 accgatcggg gaaggcggcg gcccgggact ggccagcgca cggcttgctg gcggtgacgt
     1621 ggtcgcgatt gccgggaacg tcaacgttct gccctactat ttagtcgcgc agaccaatat
     1681 caaaaagccc gaggacctgc gcggtaaaat tggcggcaat cacatcgccg gtaccaccgc
     1741 cgaattcgcg ctcaaagtcg ggcttaagaa gatcggtatc gacccgttca aggacgtcaa
     1801 tctgcgcgtg atcggcgggt cgattgaacg tatggtggcg ctgcagaagc ggattgtcga
     1861 tttcaccgtc gtcacggaag ccggcaaagc catggccgaa aagctcggct atccgaccgt
     1921 cgtcgacatg gtggcgctga agatcccgtt tccccagaat ggtattttta cgtcaaccaa
     1981 actgatccgt gataacccgg atttcgtccg tcgctacatg cgcgcctacg tcgaggcgat
     2041 ccacttttac aaaacccaca aagaagagac gattaaaacc atgcgcaagt attcgcgtgt
     2101 tgacgaccga aaatttctcg acgaggcgtg ggactggcac gtaaagttta tgccggatgc
     2161 gccgtatcct ccggccgagg gataccaact cgtcctgcag gatatggccg aaaaaaatcc
     2221 caaagcggcc caggccgaga tcaaggacta catcgatgcg cgatttgtga aggaactcga
     2281 agactcgggg tttatcaaag gattatctcg aaaatagttg atgcgtgacg taagcgtcgt
     2341 tcaaatagtt caaaacgttc aagccgttca agtcgtcaga caaaaacata gaatagaccc
     2401 gtttaaacga tttgaactgt tgaacctcga acgatttata ttttttgccg cgaagcttcg
     2461 agcacctgat gcatcggacc aacatctacc accagcgccg aaagctggag tttctcttcg
     2521 cgtaagctct cgggcacttg gctcatcact tcatcctcga gaatgtgata gacgggtatc
     2581 ttaagggcaa cgccggcaag cggtcccgcg taactcggat ctccttccgt tacggtttcc
     2641 gcgaaaactt ccacgccgtt ggcctcacca tttcctagga ttaccgtgag attttccgga
     2701 ccatactggt cc
//
